doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text	PSource	NProtein	NID	SiteSource	SiteName
29619832	6	0	gly	glycosylation	1113:1125	arg1	protein					1145:1151	this important protein	1130:1151	this important protein	1130:1151	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	8	1	contain	have	1326:1329	arg1	differences					1307:1317	The glycosylation differences	1289:1317	The glycosylation differences	1289:1317	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	8	1	contain	have	1326:1329	arg2	consequences					1357:1368	structural and functional consequences	1331:1368	structural and functional consequences	1331:1368	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	4	2	theme	kidney	820:825	arg1	cells					833:837	human embryonic kidney (HEK) cells	804:837	human embryonic kidney (HEK) cells	804:837	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	1	3	theme	Human	161:165	arg1	lysyl					167:171	Human lysyl oxidase-like 2	161:186	Human lysyl oxidase-like 2 (hLOXL2)	161:195	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	3	theme	Human	161:165	arg1	glycoprotein					200:211	a glycoprotein	198:211	a glycoprotein implicated in tumor progression and organ fibrosis	198:262	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	3	theme	Human	161:165	arg1	target					280:285	a molecular target	268:285	a molecular target for anticancer and antifibrosis treatment	268:327	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	3	theme	Human	161:165	arg1	hLOXL2					189:194	hLOXL2	189:194	hLOXL2	189:194	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	4	4	theme	glycopeptide	904:915	arg1	workflow					926:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	7	5	contain	contains	1205:1212	arg2	glycoforms					1228:1237	large, acidic glycoforms	1214:1237	large, acidic glycoforms	1214:1237	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	7	5	contain	contains	1205:1212	arg1	version					1172:1178	The more-relevant version	1154:1178	The more-relevant version	1154:1178	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	1	6	gly	glycoprotein	200:211	arg1	glycoprotein					200:211	a glycoprotein	198:211	a glycoprotein implicated in tumor progression and organ fibrosis	198:262	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	6	gly	glycoprotein	200:211	arg1	lysyl					167:171	Human lysyl oxidase-like 2	161:186	Human lysyl oxidase-like 2 (hLOXL2)	161:195	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	6	7	theme	protein	1145:1151	arg1	glycosylation					1113:1125	the glycosylation	1109:1125	the glycosylation of this important protein	1109:1151	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	8	8	theme	structural	1331:1340	arg1	consequences					1357:1368	structural and functional consequences	1331:1368	structural and functional consequences	1331:1368	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	1	9	theme	organ	249:253	arg1	fibrosis					255:262	organ fibrosis	249:262	organ fibrosis	249:262	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	0	10	theme	Complex	91:97	arg1	Glycans					110:116	Complex and Acidic Glycans	91:116	Complex and Acidic Glycans	91:116	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	6	11	theme	important	1135:1143	arg1	protein					1145:1151	this important protein	1130:1151	this important protein	1130:1151	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	2	12	contain	contains	348:355	arg1	glycoprotein					335:346	This glycoprotein	330:346	This glycoprotein	330:346	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	2	12	contain	contains	348:355	arg2	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	8	13	theme	functional	1435:1444	arg1	studies					1446:1452	functional studies	1435:1452	functional studies	1435:1452	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	7	14	theme	insect	1275:1280	arg1	cells					1282:1286	insect cells	1275:1286	insect cells	1275:1286	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	0	15	theme	Acidic	103:108	arg1	Glycans					110:116	Complex and Acidic Glycans	91:116	Complex and Acidic Glycans	91:116	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	0	16	theme	Recombinant	0:10	arg1	Lysyl					18:22	Recombinant Human Lysyl Oxidase-like 2	0:37	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells	0:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	1	17	theme	oxidase-like	173:184	arg1	lysyl					167:171	Human lysyl oxidase-like 2	161:186	Human lysyl oxidase-like 2 (hLOXL2)	161:195	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	17	theme	oxidase-like	173:184	arg1	glycoprotein					200:211	a glycoprotein	198:211	a glycoprotein implicated in tumor progression and organ fibrosis	198:262	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	17	theme	oxidase-like	173:184	arg1	target					280:285	a molecular target	268:285	a molecular target for anticancer and antifibrosis treatment	268:327	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	17	theme	oxidase-like	173:184	arg1	hLOXL2					189:194	hLOXL2	189:194	hLOXL2	189:194	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	0	18	theme	Human	12:16	arg1	Lysyl					18:22	Recombinant Human Lysyl Oxidase-like 2	0:37	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells	0:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	4	19	gly	glycosylation	755:767	arg2	sites					769:773	All three glycosylation sites	745:773	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells,	745:838	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	4	19	gly	glycosylation	755:767	arg2	three					749:753	three	749:753	three	749:753	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	7	20	theme	large	1214:1218	arg1	glycoforms					1228:1237	large, acidic glycoforms	1214:1237	large, acidic glycoforms	1214:1237	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	2	21	theme	glycosylation	382:394	arg1	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	2	22	gly	glycoprotein	335:346	arg1	glycoprotein					335:346	This glycoprotein	330:346	This glycoprotein	330:346	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	2	23	gly	glycosylation	382:394	arg2	three					357:361	three	357:361	three	357:361	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	2	23	gly	glycosylation	382:394	arg2	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	4	24	gly	glycopeptide	904:915	arg2	glycopeptide					904:915	a mass spectrometry-based glycopeptide analysis workflow	878:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	2	25	theme	N-linked	373:380	arg1	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	0	26	theme	Oxidase-like	24:35	arg1	Lysyl					18:22	Recombinant Human Lysyl Oxidase-like 2	0:37	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells	0:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	6	27	from	influence	1096:1104	arg1	glycosylation					1113:1125	the glycosylation	1109:1125	the glycosylation of this important protein	1109:1151	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	4	28	theme	embryonic	810:818	arg1	HEK					828:830	HEK	828:830	HEK	828:830	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	4	28	theme	embryonic	810:818	arg1	kidney					820:825	human embryonic kidney	804:825	human embryonic kidney (HEK) cells	804:837	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	2	29	theme	active	429:434	arg1	site					436:439	the protein's active site	415:439	the protein's active site	415:439	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	7	30	dep	large	1214:1218	arg1	acidic					1221:1226	acidic	1221:1226	acidic	1221:1226	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	5	31	gly	glycosylation	968:980	arg1	protein					1002:1008	the same protein	993:1008	the same protein expressed in insect cells	993:1034	These data were compared to the glycosylation profile of the same protein expressed in insect cells.					
29619832	6	32	theme	cell	1064:1067	arg1	type					1069:1072	the producer cell type	1051:1072	the producer cell type	1051:1072	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	1	33	theme	molecular	270:278	arg1	lysyl					167:171	Human lysyl oxidase-like 2	161:186	Human lysyl oxidase-like 2 (hLOXL2)	161:195	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	1	33	theme	molecular	270:278	arg1	target					280:285	a molecular target	268:285	a molecular target for anticancer and antifibrosis treatment	268:327	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	0	34	theme	N-Linked	131:138	arg1	Sites					154:158	All Three N-Linked Glycosylation Sites	121:158	All Three N-Linked Glycosylation Sites	121:158	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	2	35	link	N-linked	373:380	arg1	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	5	36	theme	glycosylation	968:980	arg1	profile					982:988	the glycosylation profile	964:988	the glycosylation profile of the same protein expressed in insect cells	964:1034	These data were compared to the glycosylation profile of the same protein expressed in insect cells.					
29619832	8	37	theme	functional	1346:1355	arg1	consequences					1357:1368	structural and functional consequences	1331:1368	structural and functional consequences	1331:1368	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	4	38	theme	analysis	917:924	arg1	workflow					926:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	0	39	theme	Human	53:57	arg1	Cells					76:80	Human Embryonic Kidney Cells	53:80	Human Embryonic Kidney Cells	53:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	7	40	theme	more-relevant	1158:1170	arg1	version					1172:1178	The more-relevant version	1154:1178	The more-relevant version	1154:1178	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	3	41	theme	native	599:604	arg1	profile					631:637	the native, mammalian glycosylation profile	595:637	the native, mammalian glycosylation profile	595:637	Because the glycosylation impacts the protein's biology, we sought to characterize the native, mammalian glycosylation profile and to determine how closely this profile is recapitulated when the protein is expressed in insect cells.					
29619832	4	42	theme	human	804:808	arg1	HEK					828:830	HEK	828:830	HEK	828:830	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	4	42	theme	human	804:808	arg1	kidney					820:825	human embryonic kidney	804:825	human embryonic kidney (HEK) cells	804:837	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	6	43	theme	substantial	1084:1094	arg1	influence					1096:1104	a substantial influence	1082:1104	a substantial influence on the glycosylation of this important protein	1082:1151	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	8	44	theme	screening	1477:1485	arg1	campaigns					1487:1495	high-throughput screening campaigns	1461:1495	high-throughput screening campaigns	1461:1495	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	1	45	theme	anticancer	291:300	arg1	treatment					319:327	anticancer and antifibrosis treatment	291:327	treatment	319:327	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	0	46	theme	Glycosylation	140:152	arg1	Sites					154:158	All Three N-Linked Glycosylation Sites	121:158	All Three N-Linked Glycosylation Sites	121:158	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	5	47	theme	protein	1002:1008	arg1	profile					982:988	the glycosylation profile	964:988	the glycosylation profile of the same protein expressed in insect cells	964:1034	These data were compared to the glycosylation profile of the same protein expressed in insect cells.					
29619832	5	48	theme	same	997:1000	arg1	protein					1002:1008	the same protein	993:1008	the same protein expressed in insect cells	993:1034	These data were compared to the glycosylation profile of the same protein expressed in insect cells.					
29619832	3	49	theme	insect	731:736	arg1	cells					738:742	insect cells	731:742	insect cells	731:742	Because the glycosylation impacts the protein's biology, we sought to characterize the native, mammalian glycosylation profile and to determine how closely this profile is recapitulated when the protein is expressed in insect cells.					
29619832	8	50	theme	glycosylation	1293:1305	arg1	differences					1307:1317	The glycosylation differences	1289:1317	The glycosylation differences	1289:1317	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	3	51	theme	glycosylation	617:629	arg1	profile					631:637	the native, mammalian glycosylation profile	595:637	the native, mammalian glycosylation profile	595:637	Because the glycosylation impacts the protein's biology, we sought to characterize the native, mammalian glycosylation profile and to determine how closely this profile is recapitulated when the protein is expressed in insect cells.					
29619832	4	52	from	sites	769:773	arg1	protein					782:788	the protein	778:788	the protein	778:788	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	4	53	theme	spectrometry-based	885:902	arg1	workflow					926:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	a mass spectrometry-based glycopeptide analysis workflow	878:933	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	3	54	dep	native	599:604	arg1	mammalian					607:615	mammalian	607:615	mammalian	607:615	Because the glycosylation impacts the protein's biology, we sought to characterize the native, mammalian glycosylation profile and to determine how closely this profile is recapitulated when the protein is expressed in insect cells.					
29619832	0	55	theme	Kidney	69:74	arg1	Cells					76:80	Human Embryonic Kidney Cells	53:80	Human Embryonic Kidney Cells	53:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	5	56	theme	insect	1023:1028	arg1	cells					1030:1034	insect cells	1023:1034	insect cells	1023:1034	These data were compared to the glycosylation profile of the same protein expressed in insect cells.					
29619832	8	57	theme	high-throughput	1461:1475	arg1	campaigns					1487:1495	high-throughput screening campaigns	1461:1495	high-throughput screening campaigns	1461:1495	The glycosylation differences likely have structural and functional consequences, and these data should be considered when generating protein for functional studies or for high-throughput screening campaigns.					
29619832	2	58	theme	predicted	363:371	arg1	sites					396:400	three predicted N-linked glycosylation sites	357:400	three predicted N-linked glycosylation sites	357:400	This glycoprotein contains three predicted N-linked glycosylation sites; one is near the protein's active site, and at least one more is known to facilitate the protein's secretion.					
29619832	7	59	theme	HEK	1194:1196	arg1	cells					1198:1202	HEK cells	1194:1202	HEK cells	1194:1202	The more-relevant version, expressed in HEK cells, contains large, acidic glycoforms; these glycans are not generated in insect cells.					
29619832	0	60	theme	Embryonic	59:67	arg1	Cells					76:80	Human Embryonic Kidney Cells	53:80	Human Embryonic Kidney Cells	53:80	Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.					
29619832	1	61	theme	antifibrosis	306:317	arg1	treatment					319:327	anticancer and antifibrosis treatment	291:327	treatment	319:327	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
29619832	4	62	theme	glycosylation	755:767	arg1	sites					769:773	All three glycosylation sites	745:773	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells,	745:838	All three glycosylation sites on the protein, expressed in human embryonic kidney (HEK) cells, were characterized individually using a mass spectrometry-based glycopeptide analysis workflow.					
29619832	6	63	theme	producer	1055:1062	arg1	type					1069:1072	the producer cell type	1051:1072	the producer cell type	1051:1072	We found that the producer cell type imparts a substantial influence on the glycosylation of this important protein.					
29619832	1	64	theme	tumor	227:231	arg1	progression					233:243	tumor progression	227:243	tumor progression	227:243	Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment.					
30063822	0	0	theme	Heparan	85:91	arg1	Binding					101:107	Heparan Sulfate Binding	85:107	Heparan Sulfate Binding to Robo1	85:116	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	5	1	gly	N-glycosylation	953:967	arg2	site					969:972	a single N-glycosylation site	944:972	a single N-glycosylation site in its N-terminal domains	944:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	4	2	theme	GalNAz	705:710	arg1	residue					712:718	a GalNAz residue	703:718	a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	703:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	4	2	theme	GalNAz	705:710	arg1	subject					728:734	subject	728:734	subject	728:734	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	2	3	attach	present	344:350	arg2	sites					329:333	single N-glycosylation sites	306:333	single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	306:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	2	3	attach	present	344:350	arg1	proteins					364:371	proteins	364:371	proteins	364:371	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	3	4	theme	GnT1-	530:534	arg1	cells					537:541	HEK293S (GnT1-) cells	521:541	HEK293S (GnT1-) cells	521:541	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	2	5	gly	N-glycosylation	313:327	arg2	sites					329:333	single N-glycosylation sites	306:333	single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	306:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	0	6	from	Tag	13:15	arg1	Glycoproteins					44:56	Glycoproteins	44:56	Glycoproteins	44:56	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	2	7	from	present	344:350	arg1	proteins					364:371	proteins	364:371	proteins	364:371	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	8	8	theme	docked	1452:1457	arg1	complex					1459:1465	the docked complex	1448:1465	the docked complex	1448:1465	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	7	9	theme	new	1299:1301	arg1	sulfate					1325:1331	a new high affinity heparan sulfate	1297:1331	a new high affinity heparan sulfate ligand	1297:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	7	10	theme	high	1303:1306	arg1	sulfate					1325:1331	a new high affinity heparan sulfate	1297:1331	a new high affinity heparan sulfate ligand	1297:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	4	11	theme	TEMPO	772:776	arg1	moiety					778:783	an alkyne-carrying TEMPO moiety	753:783	an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	753:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	3	12	gly	glycoproteins	504:516	arg1	glycoproteins					504:516	Recombinantly expressing glycoproteins	479:516	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells	479:541	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	0	13	theme	Sulfate	93:99	arg1	Binding					101:107	Heparan Sulfate Binding	85:107	Heparan Sulfate Binding to Robo1	85:116	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	4	14	theme	alkyne-carrying	756:770	arg1	moiety					778:783	an alkyne-carrying TEMPO moiety	753:783	an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	753:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	3	15	theme	high-mannose	569:580	arg1	structures					582:591	high-mannose structures	569:591	high-mannose structures that can be processed to leave a single GlcNAc residue	569:646	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	5	16	theme	two-domain	890:899	arg1	construct					901:909	a two-domain construct	888:909	a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains	888:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	7	17	theme	sulfate	1325:1331	arg1	ligand					1333:1338	a new high affinity heparan sulfate ligand	1297:1338	a new high affinity heparan sulfate ligand	1297:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	5	18	theme	single	946:951	arg1	site					969:972	a single N-glycosylation site	944:972	a single N-glycosylation site in its N-terminal domains	944:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	4	19	theme	enzymatic	681:689	arg1	addition					691:698	enzymatic addition	681:698	enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	681:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	6	20	theme	amide	1049:1053	arg1	nitrogens					1055:1063	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	8	21	theme	level	1432:1436	arg1	model					1438:1442	an atomic level model	1422:1442	an atomic level model for the docked complex	1422:1465	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	2	22	gly	glycoproteins	404:416	arg1	glycoproteins					404:416	glycoproteins	404:416	glycoproteins	404:416	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	3	23	from	glycoproteins	504:516	arg1	cells					537:541	HEK293S (GnT1-) cells	521:541	HEK293S (GnT1-) cells	521:541	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	5	24	theme	N-glycosylation	953:967	arg1	site					969:972	a single N-glycosylation site	944:972	a single N-glycosylation site in its N-terminal domains	944:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	5	25	from	site	969:972	arg1	domains					992:998	its N-terminal domains	977:998	its N-terminal domains	977:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	1	26	theme	site-specific	179:191	arg1	labeling					208:215	the site-specific nitroxide spin labeling	175:215	the site-specific nitroxide spin labeling of glycoproteins	175:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	2	27	theme	glycosylation	459:471	arg1	site					473:476	one glycosylation site	455:476	one glycosylation site	455:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	2	28	located	present	344:350	arg2	sites					329:333	single N-glycosylation sites	306:333	single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	306:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	2	28	located	present	344:350	arg1	proteins					364:371	proteins	364:371	proteins	364:371	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	6	29	theme	TEMPO	1200:1204	arg1	group					1206:1210	the TEMPO group	1196:1210	the TEMPO group	1196:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	2	30	from	proteins	364:371	arg1	present					344:350	present	344:350	present	344:350	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	5	31	contain	carries	936:942	arg1	protein					923:929	a protein	921:929	a protein that carries a single N-glycosylation site in its N-terminal domains	921:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	5	31	contain	carries	936:942	arg2	site					969:972	a single N-glycosylation site	944:972	a single N-glycosylation site in its N-terminal domains	944:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	5	31	contain	carries	936:942	arg1	Robo1					914:918	Robo1	914:918	Robo1	914:918	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	1	32	theme	nitroxide	193:201	arg1	labeling					208:215	the site-specific nitroxide spin labeling	175:215	the site-specific nitroxide spin labeling of glycoproteins	175:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	0	33	theme	Paramagnetic	0:11	arg1	Tag					13:15	Paramagnetic Tag	0:15	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.	0:117	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	1	34	gly	glycoproteins	220:232	arg1	glycoproteins					220:232	glycoproteins	220:232	glycoproteins	220:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	2	35	theme	sites	329:333	arg1	presence					294:301	the presence	290:301	the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	290:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	6	36	used	used	1119:1122	arg2	sites					1104:1108	sites	1104:1108	sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group	1104:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	1	37	theme	spin	203:206	arg1	labeling					208:215	the site-specific nitroxide spin labeling	175:215	the site-specific nitroxide spin labeling of glycoproteins	175:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	0	38	theme	Glycosylation	21:33	arg1	Sites					35:39	Glycosylation Sites	21:39	Glycosylation Sites	21:39	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	3	39	theme	single	626:631	arg1	residue					640:646	a single GlcNAc residue	624:646	a single GlcNAc residue	624:646	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	6	40	theme	paramagnetic	1163:1174	arg1	moiety					1186:1191	the paramagnetic nitroxide moiety	1159:1191	the paramagnetic nitroxide moiety of the TEMPO group	1159:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	2	41	theme	N-glycosylation	313:327	arg1	sites					329:333	single N-glycosylation sites	306:333	single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	306:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	7	42	theme	heparan	1317:1323	arg1	sulfate					1325:1331	a new high affinity heparan sulfate	1297:1331	a new high affinity heparan sulfate ligand	1297:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	2	43	theme	all	447:449	arg1	elimination					432:442	mutational elimination	421:442	mutational elimination of all but one glycosylation site	421:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	6	44	theme	effective	1137:1145	arg1	location					1147:1154	an effective location	1134:1154	an effective location of the paramagnetic nitroxide moiety of the TEMPO group	1134:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	2	45	theme	single	306:311	arg1	sites					329:333	single N-glycosylation sites	306:333	single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site	306:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	3	46	theme	expressing	493:502	arg1	glycoproteins					504:516	Recombinantly expressing glycoproteins	479:516	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells	479:541	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	2	47	theme	mutational	421:430	arg1	elimination					432:442	mutational elimination	421:442	mutational elimination of all but one glycosylation site	421:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	4	48	theme	click	811:815	arg1	chemistry					817:825	copper(I)-catalyzed click chemistry	791:825	copper(I)-catalyzed click chemistry	791:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	4	49	mod	modified	669:676	arg3	addition					691:698	enzymatic addition	681:698	enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	681:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	4	49	mod	modified	669:676	arg1	This					649:652	This	649:652	This	649:652	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	1	50	theme	glycoproteins	220:232	arg1	labeling					208:215	the site-specific nitroxide spin labeling	175:215	the site-specific nitroxide spin labeling of glycoproteins	175:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	3	51	with	N-glycans	554:562	arg1	structures					582:591	high-mannose structures	569:591	high-mannose structures that can be processed to leave a single GlcNAc residue	569:646	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	7	52	theme	ligand	1333:1338	arg1	spectra					1286:1292	the spectra	1282:1292	the spectra of a new high affinity heparan sulfate ligand	1282:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	0	53	from	Constraints	70:80	arg1	Binding					101:107	Heparan Sulfate Binding	85:107	Heparan Sulfate Binding to Robo1	85:116	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	4	54	theme	-catalyzed	800:809	arg1	chemistry					817:825	copper(I)-catalyzed click chemistry	791:825	copper(I)-catalyzed click chemistry	791:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	7	55	theme	affinity	1308:1315	arg1	sulfate					1325:1331	a new high affinity heparan sulfate	1297:1331	a new high affinity heparan sulfate ligand	1297:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	6	56	theme	15N	1042:1044	arg1	nitrogens					1055:1063	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	6	57	theme	nitroxide	1176:1184	arg1	moiety					1186:1191	the paramagnetic nitroxide moiety	1159:1191	the paramagnetic nitroxide moiety of the TEMPO group	1159:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	6	58	theme	residues	1075:1082	arg1	nitrogens					1055:1063	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	3	59	theme	HEK293S	521:527	arg1	cells					537:541	HEK293S (GnT1-) cells	521:541	HEK293S (GnT1-) cells	521:541	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	2	60	theme	site	473:476	arg1	elimination					432:442	mutational elimination	421:442	mutational elimination of all but one glycosylation site	421:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	4	61	with	reaction	739:746	arg1	moiety					778:783	an alkyne-carrying TEMPO moiety	753:783	an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	753:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	7	62	theme	perturbations	1265:1277	arg1	measurements					1236:1247	measurements	1236:1247	measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand	1236:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	8	63	theme	atomic	1425:1430	arg1	model					1438:1442	an atomic level model	1422:1442	an atomic level model for the docked complex	1422:1465	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	6	64	theme	group	1206:1210	arg1	moiety					1186:1191	the paramagnetic nitroxide moiety	1159:1191	the paramagnetic nitroxide moiety of the TEMPO group	1159:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	4	65	theme	residue	712:718	arg1	addition					691:698	enzymatic addition	681:698	enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry	681:825	This can in turn be modified by enzymatic addition of a GalNAz residue that is subject to reaction with an alkyne-carrying TEMPO moiety using copper(I)-catalyzed click chemistry.					
30063822	5	66	theme	N-terminal	981:990	arg1	domains					992:998	its N-terminal domains	977:998	its N-terminal domains	977:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	8	67	theme	constraints	1365:1375	arg1	Calculation					1341:1351	Calculation	1341:1351	Calculation of distance constraints from these data	1341:1391	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	6	68	theme	moiety	1186:1191	arg1	location					1147:1154	an effective location	1134:1154	an effective location of the paramagnetic nitroxide moiety of the TEMPO group	1134:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	1	69	theme	enzyme-	122:128	arg1	methodology					159:169	An enzyme- and click chemistry-mediated methodology	119:169	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins	119:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	6	70	theme	sites	1104:1108	arg1	sites					1104:1108	sites	1104:1108	sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group	1104:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	6	70	theme	sites	1104:1108	arg1	set					1097:1099	a set	1095:1099	a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group	1095:1210	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	8	71	theme	model	1438:1442	arg1	determination					1405:1417	determination	1405:1417	determination of an atomic level model for the docked complex	1405:1465	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	2	72	gly	glycosylation	459:471	arg2	site					473:476	one glycosylation site	455:476	one glycosylation site	455:476	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	2	72	gly	glycosylation	459:471	arg2	one					455:457	one	455:457	one	455:457	The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins by mutational elimination of all but one glycosylation site.					
30063822	5	73	theme	Robo1	914:918	arg1	construct					901:909	a two-domain construct	888:909	a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains	888:998	To illustrate the procedure, we have made an application to a two-domain construct of Robo1, a protein that carries a single N-glycosylation site in its N-terminal domains.					
30063822	7	74	theme	paramagnetic	1252:1263	arg1	perturbations					1265:1277	paramagnetic perturbations	1252:1277	paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand	1252:1338	This, in turn, allowed measurements of paramagnetic perturbations to the spectra of a new high affinity heparan sulfate ligand.					
30063822	8	75	theme	distance	1356:1363	arg1	constraints					1365:1375	distance constraints	1356:1375	distance constraints	1356:1375	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	6	76	theme	lysine	1068:1073	arg1	residues					1075:1082	lysine residues	1068:1082	lysine residues	1068:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	0	77	dep	Tag	13:15	arg1	Constraints					70:80	Structural Constraints	59:80	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.	0:117	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	6	78	from	nitrogens	1055:1063	arg1	15N					1042:1044	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	6	78	from	nitrogens	1055:1063	arg1	at					1046:1047	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	6	78	from	nitrogens	1055:1063	arg1	amide					1049:1053	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	1	79	theme	click	134:138	arg1	methodology					159:169	An enzyme- and click chemistry-mediated methodology	119:169	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins	119:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
30063822	6	80	theme	at	1046:1047	arg1	nitrogens					1055:1063	15N at amide nitrogens	1042:1063	15N at amide nitrogens of lysine residues	1042:1082	The construct has also been labeled with 15N at amide nitrogens of lysine residues to provide a set of sites that are used to derive an effective location of the paramagnetic nitroxide moiety of the TEMPO group.					
30063822	8	81	from	data	1388:1391	arg1	Calculation					1341:1351	Calculation	1341:1351	Calculation of distance constraints from these data	1341:1391	Calculation of distance constraints from these data facilitated determination of an atomic level model for the docked complex.					
30063822	0	82	theme	Structural	59:68	arg1	Constraints					70:80	Structural Constraints	59:80	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.	0:117	Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1.					
30063822	3	83	theme	GlcNAc	633:638	arg1	residue					640:646	a single GlcNAc residue	624:646	a single GlcNAc residue	624:646	Recombinantly expressing glycoproteins in HEK293S (GnT1-) cells results in N-glycans with high-mannose structures that can be processed to leave a single GlcNAc residue.					
30063822	1	84	theme	chemistry-mediated	140:157	arg1	methodology					159:169	An enzyme- and click chemistry-mediated methodology	119:169	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins	119:232	An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied.					
29642453	8	0	theme	influenza	1485:1493	arg1	surveillance					1495:1506	influenza surveillance	1485:1506	influenza surveillance	1485:1506	The results provided the rationale for establishing the ecological niche of influenza viruses among the reservoir and could be implemented for influenza surveillance and improving pandemic preparedness.					
29642453	6	1	theme	recent	956:961	arg1	isolates					963:970	recent isolates	956:970	recent isolates	956:970	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	3	2	theme	HA	480:481	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	2	theme	HA	480:481	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	2	theme	HA	480:481	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	1	3	theme	crucial	240:246	arg1	means					248:252	crucial means	240:252	crucial means for immune evasion and viral fitness	240:289	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	5	4	theme	transmission	804:815	arg1	Co-segregation					708:721	Co-segregation	708:721	Co-segregation of particular glycosylation sites	708:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	4	theme	transmission	804:815	arg1	characteristic					777:790	a characteristic	775:790	a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations	775:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	5	from	reservoirs	829:838	arg1	Co-segregation					708:721	Co-segregation	708:721	Co-segregation of particular glycosylation sites	708:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	5	from	reservoirs	829:838	arg1	characteristic					777:790	a characteristic	775:790	a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations	775:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	5	from	reservoirs	829:838	arg1	transmission					804:815	zoonotic transmission	795:815	zoonotic transmission from animal reservoirs	795:838	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	1	6	theme	hemagglutinin	172:184	arg1	Glycosylation					151:163	Glycosylation	151:163	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza	151:229	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	8	7	theme	viruses	1428:1434	arg1	niche					1409:1413	the ecological niche	1394:1413	the ecological niche of influenza viruses among the reservoir	1394:1454	The results provided the rationale for establishing the ecological niche of influenza viruses among the reservoir and could be implemented for influenza surveillance and improving pandemic preparedness.					
29642453	7	8	theme	immune	1256:1261	arg1	evasion					1263:1269	immune evasion	1256:1269	immune evasion	1256:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	4	9	gly	glycosylation	603:615	arg2	sites					617:621	glycosylation sites	603:621	glycosylation sites	603:621	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	7	10	theme	HA	1240:1241	arg1	epitopes					1243:1250	multiple HA epitopes	1231:1250	multiple HA epitopes for immune evasion	1231:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	5	11	theme	"	880:880	arg1	Co-segregation					708:721	Co-segregation	708:721	Co-segregation of particular glycosylation sites	708:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	11	theme	"	880:880	arg1	characteristic					777:790	a characteristic	775:790	a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations	775:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	0	12	theme	Ecological	87:96	arg1	Spillover					98:106	Ecological Spillover	87:106	Ecological Spillover	87:106	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	8	13	theme	pandemic	1522:1529	arg1	preparedness					1531:1542	pandemic preparedness	1522:1542	pandemic preparedness	1522:1542	The results provided the rationale for establishing the ecological niche of influenza viruses among the reservoir and could be implemented for influenza surveillance and improving pandemic preparedness.					
29642453	5	14	theme	human	885:889	arg1	viruses					891:897	human viruses	885:897	human viruses	885:897	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	1	15	theme	immune	258:263	arg1	evasion					265:271	immune evasion	258:271	immune evasion	258:271	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	6	16	theme	canonical	997:1005	arg1	sites					1007:1011	canonical sites	997:1011	canonical sites in HA	997:1017	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	3	17	theme	H1N1	493:496	arg1	subtype					498:504	H1N1 subtype	493:504	H1N1 subtype isolated from human, avian, and swine species over the past century	493:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	5	18	theme	zoonotic	795:802	arg1	transmission					804:815	zoonotic transmission	795:815	zoonotic transmission from animal reservoirs	795:838	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	3	19	theme	potential	420:428	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	19	theme	potential	420:428	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	19	theme	potential	420:428	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	8	20	theme	ecological	1398:1407	arg1	niche					1409:1413	the ecological niche	1394:1413	the ecological niche of influenza viruses among the reservoir	1394:1454	The results provided the rationale for establishing the ecological niche of influenza viruses among the reservoir and could be implemented for influenza surveillance and improving pandemic preparedness.					
29642453	6	21	gly	glycosylation	1072:1084	arg1	NA					1089:1090	NA	1089:1090	NA	1089:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	3	22	theme	subtype	498:504	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	22	theme	subtype	498:504	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	23	theme	N-linked	430:437	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	23	theme	N-linked	430:437	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	23	theme	N-linked	430:437	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	6	24	from	sites	1007:1011	arg1	HA					1016:1017	HA	1016:1017	HA	1016:1017	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	25	theme	novel	1066:1070	arg1	signature					1110:1118	an independent signature	1095:1118	an independent signature for adaptation among humans	1095:1146	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	25	theme	novel	1066:1070	arg1	glycosylation					1072:1084	a novel glycosylation	1064:1084	a novel glycosylation in NA	1064:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	25	theme	novel	1066:1070	arg1	adaptation					1048:1057	gradual seasonal adaptation	1031:1057	gradual seasonal adaptation	1031:1057	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	2	26	gly	glycosylation	358:370	arg2	sites					372:376	each glycosylation sites	353:376	each glycosylation sites	353:376	However, the time-dependent dynamics of each glycosylation sites have not been addressed.					
29642453	3	27	theme	avian	527:531	arg1	species					544:550	human, avian, and swine species	520:550	human, avian, and swine species	520:550	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	4	28	from	shift	594:598	arg1	sites					617:621	glycosylation sites	603:621	glycosylation sites	603:621	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	2	29	theme	sites	372:376	arg1	dynamics					341:348	the time-dependent dynamics	322:348	the time-dependent dynamics of each glycosylation sites	322:376	However, the time-dependent dynamics of each glycosylation sites have not been addressed.					
29642453	5	30	theme	particular	726:735	arg1	sites					751:755	particular glycosylation sites	726:755	particular glycosylation sites	726:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	3	31	link	N-linked	430:437	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	31	link	N-linked	430:437	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	31	link	N-linked	430:437	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	7	32	from	influence	1207:1215	arg1	epitopes					1243:1250	multiple HA epitopes	1231:1250	multiple HA epitopes for immune evasion	1231:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	5	33	theme	reverse	864:870	arg1	"					880:880	"reverse zoonosis"	863:880	"reverse zoonosis" of human viruses into swine populations	863:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	2	34	theme	glycosylation	358:370	arg1	sites					372:376	each glycosylation sites	353:376	each glycosylation sites	353:376	However, the time-dependent dynamics of each glycosylation sites have not been addressed.					
29642453	5	35	theme	glycosylation	737:749	arg1	sites					751:755	particular glycosylation sites	726:755	particular glycosylation sites	726:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	0	36	theme	Influenza	129:137	arg1	Reservoirs					139:148	Influenza Reservoirs	129:148	Influenza Reservoirs	129:148	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	3	37	theme	swine	538:542	arg1	species					544:550	human, avian, and swine species	520:550	human, avian, and swine species	520:550	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	38	theme	human	520:524	arg1	species					544:550	human, avian, and swine species	520:550	human, avian, and swine species	520:550	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	39	attach	isolated	506:513	arg1	species					544:550	human, avian, and swine species	520:550	human, avian, and swine species	520:550	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	39	attach	isolated	506:513	arg2	subtype					498:504	H1N1 subtype	493:504	H1N1 subtype isolated from human, avian, and swine species over the past century	493:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	1	40	theme	neuraminidase	195:207	arg1	Glycosylation					151:163	Glycosylation	151:163	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza	151:229	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	0	41	theme	Hemagglutinin	17:29	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.	0:149	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	1	42	theme	viral	277:281	arg1	fitness					283:289	viral fitness	277:289	viral fitness	277:289	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	4	43	theme	abortive	688:695	arg1	"					705:705	the 1976 "abortive pandemic"	678:705	the 1976 "abortive pandemic"	678:705	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	2	44	theme	time-dependent	326:339	arg1	dynamics					341:348	the time-dependent dynamics	322:348	the time-dependent dynamics of each glycosylation sites	322:376	However, the time-dependent dynamics of each glycosylation sites have not been addressed.					
29642453	3	45	theme	NA	487:488	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	45	theme	NA	487:488	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	45	theme	NA	487:488	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	6	46	from	glycosylation	1072:1084	arg1	NA					1089:1090	NA	1089:1090	NA	1089:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	7	47	theme	glycans	1220:1226	arg1	influence					1207:1215	a remarkably pleiotropic influence	1182:1215	a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion	1182:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	0	48	gly	Glycosylation	0:12	arg1	Hemagglutinin					17:29	Hemagglutinin	17:29	Hemagglutinin	17:29	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	0	48	gly	Glycosylation	0:12	arg1	Neuraminidase					35:47	Neuraminidase	35:47	Neuraminidase	35:47	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	4	49	theme	glycosylation	603:615	arg1	sites					617:621	glycosylation sites	603:621	glycosylation sites	603:621	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	3	50	theme	glycosylation	439:451	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	50	theme	glycosylation	439:451	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	50	theme	glycosylation	439:451	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	0	51	theme	Neuraminidase	35:47	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.	0:149	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	7	52	theme	HA	1316:1317	arg1	activity					1326:1333	the activity	1322:1333	the activity of NA	1322:1339	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	7	52	theme	HA	1316:1317	arg1	binding					1305:1311	the receptor binding	1292:1311	the receptor binding of HA	1292:1317	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	0	53	theme	A	62:62	arg1	Virus					64:68	Influenza A Virus	52:68	Influenza A Virus	52:68	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	5	54	theme	sites	751:755	arg1	Co-segregation					708:721	Co-segregation	708:721	Co-segregation of particular glycosylation sites	708:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	54	theme	sites	751:755	arg1	well					925:928	well	925:928	well	925:928	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	54	theme	sites	751:755	arg1	characteristic					777:790	a characteristic	775:790	a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations	775:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	7	55	theme	receptor	1296:1303	arg1	binding					1305:1311	the receptor binding	1292:1311	the receptor binding of HA	1292:1317	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	0	56	theme	Influenza	52:60	arg1	Virus					64:68	Influenza A Virus	52:68	Influenza A Virus	52:68	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	1	57	theme	host	296:299	arg1	population					301:310	a host population	294:310	a host population	294:310	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	1	58	gly	Glycosylation	151:163	arg1	influenza					221:229	the influenza	217:229	the influenza	217:229	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	1	58	gly	Glycosylation	151:163	arg1	NA					210:211	NA	210:211	NA	210:211	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	1	58	gly	Glycosylation	151:163	arg1	HA					187:188	HA	187:188	HA	187:188	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	1	58	gly	Glycosylation	151:163	arg1	neuraminidase					195:207	neuraminidase	195:207	neuraminidase (NA)	195:212	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	1	58	gly	Glycosylation	151:163	arg1	hemagglutinin					172:184	the hemagglutinin	168:184	the hemagglutinin (HA)	168:189	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	3	59	gly	glycosylation	439:451	arg2	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	59	gly	glycosylation	439:451	arg2	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	59	gly	glycosylation	439:451	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	59	gly	glycosylation	439:451	arg2	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	59	gly	glycosylation	439:451	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	6	60	from	adaptation	1048:1057	arg1	NA					1089:1090	NA	1089:1090	NA	1089:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	61	theme	seasonal	1039:1046	arg1	signature					1110:1118	an independent signature	1095:1118	an independent signature for adaptation among humans	1095:1146	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	61	theme	seasonal	1039:1046	arg1	glycosylation					1072:1084	a novel glycosylation	1064:1084	a novel glycosylation in NA	1064:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	61	theme	seasonal	1039:1046	arg1	adaptation					1048:1057	gradual seasonal adaptation	1031:1057	gradual seasonal adaptation	1031:1057	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	5	62	theme	swine	904:908	arg1	populations					910:920	swine populations	904:920	swine populations	904:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	63	gly	glycosylation	737:749	arg2	sites					751:755	particular glycosylation sites	726:755	particular glycosylation sites	726:755	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	7	64	theme	NA	1338:1339	arg1	activity					1326:1333	the activity	1322:1333	the activity of NA	1322:1339	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	7	64	theme	NA	1338:1339	arg1	binding					1305:1311	the receptor binding	1292:1311	the receptor binding of HA	1292:1317	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	4	65	theme	pandemics	654:662	arg1	hallmark					628:635	a hallmark	626:635	a hallmark of 1918 and 2009 pandemics	626:662	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	4	65	theme	pandemics	654:662	arg1	shift					594:598	a shift	592:598	a shift in glycosylation sites	592:621	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	5	66	theme	zoonosis	872:879	arg1	"					880:880	"reverse zoonosis"	863:880	"reverse zoonosis" of human viruses into swine populations	863:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	6	67	gly	glycosylation	980:992	arg1	sites					1007:1011	canonical sites	997:1011	canonical sites in HA	997:1017	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	4	68	theme	pandemic	697:704	arg1	"					705:705	the 1976 "abortive pandemic"	678:705	the 1976 "abortive pandemic"	678:705	The results show a shift in glycosylation sites as a hallmark of 1918 and 2009 pandemics, and also for the 1976 "abortive pandemic".					
29642453	7	69	theme	pleiotropic	1195:1205	arg1	influence					1207:1215	a remarkably pleiotropic influence	1182:1215	a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion	1182:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	6	70	theme	gradual	1031:1037	arg1	signature					1110:1118	an independent signature	1095:1118	an independent signature for adaptation among humans	1095:1146	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	70	theme	gradual	1031:1037	arg1	glycosylation					1072:1084	a novel glycosylation	1064:1084	a novel glycosylation in NA	1064:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	70	theme	gradual	1031:1037	arg1	adaptation					1048:1057	gradual seasonal adaptation	1031:1057	gradual seasonal adaptation	1031:1057	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	5	71	theme	viruses	891:897	arg1	"					880:880	"reverse zoonosis"	863:880	"reverse zoonosis" of human viruses into swine populations	863:920	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	6	72	theme	independent	1098:1108	arg1	signature					1110:1118	an independent signature	1095:1118	an independent signature for adaptation among humans	1095:1146	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	72	theme	independent	1098:1108	arg1	glycosylation					1072:1084	a novel glycosylation	1064:1084	a novel glycosylation in NA	1064:1090	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	6	72	theme	independent	1098:1108	arg1	adaptation					1048:1057	gradual seasonal adaptation	1031:1057	gradual seasonal adaptation	1031:1057	After the 2009 pandemic, recent isolates accrued glycosylation at canonical sites in HA, reflecting gradual seasonal adaptation, and a novel glycosylation in NA as an independent signature for adaptation among humans.					
29642453	3	73	theme	NLG	454:456	arg1	sites					459:463	the potential N-linked glycosylation (NLG) sites	416:463	the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century	416:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	73	theme	NLG	454:456	arg1	NA					487:488	NA	487:488	NA	487:488	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	3	73	theme	NLG	454:456	arg1	HA					480:481	over 10,000 HA	468:481	over 10,000 HA	468:481	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	2	74	theme	each	353:356	arg1	sites					372:376	each glycosylation sites	353:376	each glycosylation sites	353:376	However, the time-dependent dynamics of each glycosylation sites have not been addressed.					
29642453	7	75	theme	multiple	1231:1238	arg1	epitopes					1243:1250	multiple HA epitopes	1231:1250	multiple HA epitopes for immune evasion	1231:1269	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29642453	1	76	theme	influenza	221:229	arg1	Glycosylation					151:163	Glycosylation	151:163	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza	151:229	Glycosylation of the hemagglutinin (HA) and neuraminidase (NA) of the influenza provides crucial means for immune evasion and viral fitness in a host population.					
29642453	0	77	theme	Virus	64:68	arg1	Hemagglutinin					17:29	Hemagglutinin	17:29	Hemagglutinin	17:29	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	0	77	theme	Virus	64:68	arg1	Neuraminidase					35:47	Neuraminidase	35:47	Neuraminidase	35:47	Glycosylation of Hemagglutinin and Neuraminidase of Influenza A Virus as Signature for Ecological Spillover and Adaptation among Influenza Reservoirs.					
29642453	8	78	theme	influenza	1418:1426	arg1	viruses					1428:1434	influenza viruses	1418:1434	influenza viruses	1418:1434	The results provided the rationale for establishing the ecological niche of influenza viruses among the reservoir and could be implemented for influenza surveillance and improving pandemic preparedness.					
29642453	3	79	theme	past	561:564	arg1	century					566:572	the past century	557:572	the past century	557:572	We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century.					
29642453	5	80	from	characteristic	777:790	arg1	reservoirs					829:838	animal reservoirs	822:838	animal reservoirs	822:838	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	5	81	theme	animal	822:827	arg1	reservoirs					829:838	animal reservoirs	822:838	animal reservoirs	822:838	Co-segregation of particular glycosylation sites was identified as a characteristic of zoonotic transmission from animal reservoirs, and interestingly, of "reverse zoonosis" of human viruses into swine populations as well.					
29642453	7	82	theme	Structural	1149:1158	arg1	predictions					1160:1170	Structural predictions	1149:1170	Structural predictions	1149:1170	Structural predictions indicated a remarkably pleiotropic influence of glycans on multiple HA epitopes for immune evasion, without sacrificing the receptor binding of HA or the activity of NA.					
29405331	8	0	theme	three-step	1070:1079	arg1	strategy					1113:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	6	1	theme	glycopeptides	911:923	arg1	quantification					893:906	quantification	893:906	quantification	893:906	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	6	1	theme	glycopeptides	911:923	arg1	identification					874:887	identification	874:887	identification	874:887	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	8	2	theme	spectrometry	1086:1097	arg1	strategy					1113:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	10	3	theme	N-glycan	1509:1516	arg1	structures					1518:1527	complex N-glycan structures	1501:1527	complex N-glycan structures	1501:1527	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	1	4	gly	antennary-fucosylated	196:216	arg1	glycosylation					218:230	antennary-fucosylated glycosylation	196:230	antennary-fucosylated glycosylation	196:230	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	4	5	theme	similar	710:716	arg1	structures					725:734	similar glycan structures	710:734	similar glycan structures	710:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	9	6	theme	ions	1350:1353	arg1	detection					1329:1337	improved detection	1320:1337	improved detection of oxonium ions	1320:1353	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	9	6	theme	ions	1350:1353	arg1	selection					1366:1374	better selection	1359:1374	better selection of Y1 ion (peptide+GlcNAc)	1359:1401	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	2	7	theme	possible	308:315	arg1	occupancy					324:332	multiple possible glycan occupancy	299:332	multiple possible glycan occupancy at each site (microheterogeneity)	299:366	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	7	theme	possible	308:315	arg1	microheterogeneity					348:365	microheterogeneity	348:365	microheterogeneity	348:365	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	10	8	contain	contains	1604:1611	arg2	glycans					1643:1649	tetra-antennary glycans	1627:1649	tetra-antennary glycans	1627:1649	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	8	contain	contains	1604:1611	arg2	bi-					1613:1615	bi-	1613:1615	bi-	1613:1615	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	8	contain	contains	1604:1611	arg2	tri-					1618:1621	tri-	1618:1621	tri-	1618:1621	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	8	contain	contains	1604:1611	arg1	site					1592:1595	site Asn107	1592:1602	site Asn107	1592:1602	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	9	with	glycans	1738:1744	arg1	antennary-fucosylation					1766:1787	antennary-fucosylation	1766:1787	antennary-fucosylation	1766:1787	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	9	with	glycans	1738:1744	arg1	core-					1756:1760	core-	1756:1760	core-	1756:1760	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	4	10	gly	glycopeptides	691:703	arg2	glycopeptides					691:703	glycopeptides	691:703	glycopeptides with similar glycan structures	691:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	9	11	theme	better	1359:1364	arg1	selection					1366:1374	better selection	1359:1374	better selection of Y1 ion (peptide+GlcNAc)	1359:1401	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	8	12	theme	glycopeptide	1135:1146	arg1	identification					1148:1161	glycopeptide identification	1135:1161	glycopeptide identification	1135:1161	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	9	13	theme	improved	1320:1327	arg1	detection					1329:1337	improved detection	1320:1337	improved detection of oxonium ions	1320:1353	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	11	14	theme	other	1973:1977	arg1	sites					1983:1987	the other two sites	1969:1987	the other two sites	1969:1987	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	1	15	theme	glycopeptides	235:247	arg1	core-					186:190	core-	186:190	core-	186:190	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	1	15	theme	glycopeptides	235:247	arg1	glycosylation					218:230	antennary-fucosylated glycosylation	196:230	antennary-fucosylated glycosylation	196:230	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	4	16	theme	glycopeptides	691:703	arg1	signal					681:686	the MS signal	674:686	the MS signal of glycopeptides with similar glycan structures	674:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	9	17	theme	ion	1382:1384	arg1	detection					1329:1337	improved detection	1320:1337	improved detection of oxonium ions	1320:1353	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	9	17	theme	ion	1382:1384	arg1	selection					1366:1374	better selection	1359:1374	better selection of Y1 ion (peptide+GlcNAc)	1359:1401	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	6	18	gly	glycopeptides	911:923	arg2	glycopeptides					911:923	glycopeptides	911:923	glycopeptides with low abundance glycan modification	911:962	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	2	19	gly	glycosylation	259:271	arg2	site					342:345	each site	337:345	each site	337:345	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	19	gly	glycosylation	259:271	arg2	heterogeneity					280:292	heterogeneity	280:292	heterogeneity	280:292	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	19	gly	glycosylation	259:271	arg2	sites					273:277	the glycosylation sites	255:277	the glycosylation sites (heterogeneity)	255:293	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	5	20	theme	β1-3,4	737:742	arg1	galactosidase					744:756	β1-3,4 galactosidase	737:756	β1-3,4 galactosidase	737:756	β1-3,4 galactosidase was used to differentiate core- and antennary-fucosylation.					
29405331	1	21	theme	-based	131:136	arg1	methodology					138:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	11	22	theme	Asn107	1942:1947	arg1	larger					1957:1962	larger	1957:1962	larger	1957:1962	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	22	theme	Asn107	1942:1947	arg1	level					1933:1937	the glycosylation level	1915:1937	the glycosylation level of Asn107	1915:1947	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	2	23	from	site	342:345	arg1	sites					273:277	the glycosylation sites	255:277	the glycosylation sites (heterogeneity)	255:293	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	23	from	site	342:345	arg1	occupancy					324:332	multiple possible glycan occupancy	299:332	multiple possible glycan occupancy at each site (microheterogeneity)	299:366	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	23	from	site	342:345	arg1	heterogeneity					280:292	heterogeneity	280:292	heterogeneity	280:292	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	23	from	site	342:345	arg1	microheterogeneity					348:365	microheterogeneity	348:365	microheterogeneity	348:365	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	8	24	theme	software	1186:1193	arg1	annotation					1195:1204	pGlyco software annotation	1179:1204	pGlyco software annotation	1179:1204	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	7	25	theme	in-source	1044:1052	arg1	dissociation					1054:1065	the in-source dissociation	1040:1065	the in-source dissociation	1040:1065	The settings of the mass spectrometer were therefore optimized to minimize the in-source dissociation.					
29405331	1	26	theme	chromatography-tandem	81:101	arg1	spectrometry					108:119	liquid chromatography-tandem mass spectrometry	74:119	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	1	26	theme	chromatography-tandem	81:101	arg1	LC-MS/MS					122:129	LC-MS/MS	122:129	LC-MS/MS	122:129	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	3	27	theme	serum	422:426	arg1	glycoprotein					428:439	The serum glycoprotein alpha-1-antitrypsin	418:459	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites	418:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	27	theme	serum	422:426	arg1	A1AT					462:465	A1AT	462:465	A1AT	462:465	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	9	28	theme	collision	1231:1239	arg1	crucial					1308:1314	crucial	1308:1314	crucial	1308:1314	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	9	28	theme	collision	1231:1239	arg1	energy					1241:1246	The collision energy	1227:1246	The collision energy used for initial glycopeptide fragmentation	1227:1290	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	4	29	used	used	569:572	arg2	Sialidase					555:563	Sialidase	555:563	Sialidase	555:563	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	1	30	theme	spectrometry	108:119	arg1	methodology					138:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	2	31	gly	glycopeptide	395:406	arg2	glycopeptide					395:406	intact glycopeptide analysis	388:415	intact glycopeptide analysis	388:415	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	10	32	gly	antennary-fucosylation	1766:1787	arg1	bi-					1716:1718	bi-	1716:1718	bi-	1716:1718	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	32	gly	antennary-fucosylation	1766:1787	arg1	glycans					1738:1744	tri-antennary glycans	1724:1744	tri-antennary glycans with both core- and antennary-fucosylation	1724:1787	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	33	contain	contain	1493:1499	arg2	structures					1518:1527	complex N-glycan structures	1501:1527	complex N-glycan structures	1501:1527	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	33	contain	contain	1493:1499	arg1	sites					1465:1469	all three glycosylation sites	1441:1469	all three glycosylation sites of A1AT glycopeptides	1441:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	33	contain	contain	1493:1499	arg1	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	34	with	glycans	1643:1649	arg1	core-					1661:1665	core-	1661:1665	core-	1661:1665	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	34	with	glycans	1643:1649	arg1	antennary-fucosylation					1671:1692	antennary-fucosylation	1671:1692	antennary-fucosylation	1671:1692	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	9	35	theme	initial	1257:1263	arg1	fragmentation					1278:1290	initial glycopeptide fragmentation	1257:1290	initial glycopeptide fragmentation	1257:1290	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	0	36	theme	glycosylation	36:48	arg1	analysis					4:11	The analysis	0:11	The analysis of alpha-1-antitrypsin glycosylation with direct LC-MS/MS	0:69	The analysis of alpha-1-antitrypsin glycosylation with direct LC-MS/MS.					
29405331	3	37	theme	alpha-1-antitrypsin	441:459	arg1	glycoprotein					428:439	The serum glycoprotein alpha-1-antitrypsin	418:459	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites	418:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	37	theme	alpha-1-antitrypsin	441:459	arg1	A1AT					462:465	A1AT	462:465	A1AT	462:465	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	38	gly	antennary-fucosylated	498:518	arg1	glycosites					520:529	antennary-fucosylated glycosites	498:529	antennary-fucosylated glycosites	498:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	0	39	theme	direct	55:60	arg1	LC-MS/MS					62:69	direct LC-MS/MS	55:69	direct LC-MS/MS	55:69	The analysis of alpha-1-antitrypsin glycosylation with direct LC-MS/MS.					
29405331	6	40	theme	glycan	944:949	arg1	modification					951:962	low abundance glycan modification	930:962	low abundance glycan modification	930:962	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	11	41	theme	glycosylation	1919:1931	arg1	larger					1957:1962	larger	1957:1962	larger	1957:1962	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	41	theme	glycosylation	1919:1931	arg1	level					1933:1937	the glycosylation level	1915:1937	the glycosylation level of Asn107	1915:1947	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	10	42	theme	A1AT	1474:1477	arg1	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	6	43	theme	low	930:932	arg1	modification					951:962	low abundance glycan modification	930:962	low abundance glycan modification	930:962	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	10	44	theme	complex	1501:1507	arg1	structures					1518:1527	complex N-glycan structures	1501:1527	complex N-glycan structures	1501:1527	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	6	45	dep	identification	874:887	arg1	the					870:872	the	870:872	the	870:872	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	10	46	theme	tri-antennary	1724:1736	arg1	glycans					1738:1744	tri-antennary glycans	1724:1744	tri-antennary glycans with both core- and antennary-fucosylation	1724:1787	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	11	47	theme	core-	1816:1820	arg1	intensity					1803:1811	The relative intensity	1790:1811	The relative intensity of core- and antennary-fucosylation on Asn107	1790:1857	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	47	theme	core-	1816:1820	arg1	similar					1863:1869	similar	1863:1869	similar	1863:1869	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	48	from	intensity	1803:1811	arg1	Asn107					1852:1857	Asn107	1852:1857	Asn107	1852:1857	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	9	49	gly	glycopeptide	1265:1276	arg2	glycopeptide					1265:1276	initial glycopeptide fragmentation	1257:1290	initial glycopeptide fragmentation	1257:1290	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	8	50	theme	mass	1081:1084	arg1	strategy					1113:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	0	51	with	glycosylation	36:48	arg1	LC-MS/MS					62:69	direct LC-MS/MS	55:69	direct LC-MS/MS	55:69	The analysis of alpha-1-antitrypsin glycosylation with direct LC-MS/MS.					
29405331	2	52	theme	intact	388:393	arg1	analysis					408:415	intact glycopeptide analysis	388:415	intact glycopeptide analysis	388:415	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	7	53	theme	spectrometer	990:1001	arg1	settings					969:976	The settings	965:976	The settings of the mass spectrometer	965:1001	The settings of the mass spectrometer were therefore optimized to minimize the in-source dissociation.					
29405331	8	54	theme	fragmentation	1099:1111	arg1	strategy					1113:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	11	55	theme	antennary-fucosylation	1826:1847	arg1	intensity					1803:1811	The relative intensity	1790:1811	The relative intensity of core- and antennary-fucosylation on Asn107	1790:1857	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	55	theme	antennary-fucosylation	1826:1847	arg1	similar					1863:1869	similar	1863:1869	similar	1863:1869	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	1	56	gly	glycosylation	218:230	arg1	glycopeptides					235:247	glycopeptides	235:247	glycopeptides	235:247	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	10	57	theme	biantennary	1550:1560	arg1	glycans					1562:1568	biantennary glycans	1550:1568	biantennary glycans	1550:1568	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	4	58	theme	glycosylation	626:638	arg1	microheterogeneity					640:657	the glycosylation microheterogeneity	622:657	the glycosylation microheterogeneity	622:657	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	1	59	gly	glycopeptides	235:247	arg2	glycopeptides					235:247	glycopeptides	235:247	glycopeptides	235:247	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	9	60	theme	oxonium	1342:1348	arg1	ions					1350:1353	oxonium ions	1342:1353	oxonium ions	1342:1353	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	2	61	theme	glycan	317:322	arg1	occupancy					324:332	multiple possible glycan occupancy	299:332	multiple possible glycan occupancy at each site (microheterogeneity)	299:366	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	61	theme	glycan	317:322	arg1	microheterogeneity					348:365	microheterogeneity	348:365	microheterogeneity	348:365	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	10	62	gly	antennary-fucosylation	1671:1692	arg1	bi-					1613:1615	bi-	1613:1615	bi-	1613:1615	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	62	gly	antennary-fucosylation	1671:1692	arg1	tri-					1618:1621	tri-	1618:1621	tri-	1618:1621	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	62	gly	antennary-fucosylation	1671:1692	arg1	glycans					1643:1649	tetra-antennary glycans	1627:1649	tetra-antennary glycans	1627:1649	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	1	63	theme	antennary-fucosylated	196:216	arg1	glycosylation					218:230	antennary-fucosylated glycosylation	196:230	antennary-fucosylated glycosylation	196:230	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	4	64	theme	glycan	718:723	arg1	structures					725:734	similar glycan structures	710:734	similar glycan structures	710:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	6	65	with	glycopeptides	911:923	arg1	modification					951:962	low abundance glycan modification	930:962	low abundance glycan modification	930:962	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	8	66	used	used	1126:1129	arg2	strategy					1113:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy	1068:1120	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	4	67	theme	MS	678:679	arg1	signal					681:686	the MS signal	674:686	the MS signal of glycopeptides with similar glycan structures	674:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	9	68	theme	Y1	1379:1380	arg1	ion					1382:1384	Y1 ion	1379:1384	Y1 ion (peptide+GlcNAc)	1379:1401	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	9	68	theme	Y1	1379:1380	arg1	peptide+GlcNAc					1387:1400	peptide+GlcNAc	1387:1400	peptide+GlcNAc	1387:1400	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	10	69	gly	glycosylation	1451:1463	arg2	sites					1465:1469	all three glycosylation sites	1441:1469	all three glycosylation sites of A1AT glycopeptides	1441:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	69	gly	glycosylation	1451:1463	arg1	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	69	gly	glycosylation	1451:1463	arg2	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	69	gly	glycosylation	1451:1463	arg2	three					1445:1449	three	1445:1449	three	1445:1449	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	8	70	gly	glycopeptide	1135:1146	arg2	glycopeptide					1135:1146	glycopeptide identification	1135:1161	glycopeptide identification	1135:1161	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	2	71	theme	glycosylation	259:271	arg1	sites					273:277	the glycosylation sites	255:277	the glycosylation sites (heterogeneity)	255:293	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	71	theme	glycosylation	259:271	arg1	heterogeneity					280:292	heterogeneity	280:292	heterogeneity	280:292	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	5	72	used	used	762:765	arg2	galactosidase					744:756	β1-3,4 galactosidase	737:756	β1-3,4 galactosidase	737:756	β1-3,4 galactosidase was used to differentiate core- and antennary-fucosylation.					
29405331	3	73	contain	contains	474:481	arg1	A1AT					462:465	A1AT	462:465	A1AT	462:465	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	73	contain	contains	474:481	arg1	glycoprotein					428:439	The serum glycoprotein alpha-1-antitrypsin	418:459	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites	418:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	73	contain	contains	474:481	arg2	core-					488:492	core-	488:492	core-	488:492	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	73	contain	contains	474:481	arg2	glycosites					520:529	antennary-fucosylated glycosites	498:529	antennary-fucosylated glycosites	498:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	2	74	theme	multiple	299:306	arg1	occupancy					324:332	multiple possible glycan occupancy	299:332	multiple possible glycan occupancy at each site (microheterogeneity)	299:366	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	2	74	theme	multiple	299:306	arg1	microheterogeneity					348:365	microheterogeneity	348:365	microheterogeneity	348:365	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	10	75	theme	Structural	1404:1413	arg1	assignments					1415:1425	Structural assignments	1404:1425	Structural assignments	1404:1425	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	8	76	theme	pGlyco	1179:1184	arg1	annotation					1195:1204	pGlyco software annotation	1179:1204	pGlyco software annotation	1179:1204	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	10	77	contain	contains	1707:1714	arg1	site					1695:1698	site Asn271	1695:1705	site Asn271	1695:1705	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	77	contain	contains	1707:1714	arg2	glycans					1738:1744	tri-antennary glycans	1724:1744	tri-antennary glycans with both core- and antennary-fucosylation	1724:1787	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	77	contain	contains	1707:1714	arg2	bi-					1716:1718	bi-	1716:1718	bi-	1716:1718	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	3	78	gly	glycoprotein	428:439	arg1	glycoprotein					428:439	The serum glycoprotein alpha-1-antitrypsin	418:459	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites	418:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	78	gly	glycoprotein	428:439	arg1	A1AT					462:465	A1AT	462:465	A1AT	462:465	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	1	79	theme	liquid	74:79	arg1	spectrometry					108:119	liquid chromatography-tandem mass spectrometry	74:119	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	1	79	theme	liquid	74:79	arg1	LC-MS/MS					122:129	LC-MS/MS	122:129	LC-MS/MS	122:129	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	10	80	gly	glycopeptides	1479:1491	arg2	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	81	theme	tetra-antennary	1627:1641	arg1	glycans					1643:1649	tetra-antennary glycans	1627:1649	tetra-antennary glycans	1627:1649	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	8	82	theme	manual	1210:1215	arg1	checking					1217:1224	manual checking	1210:1224	manual checking	1210:1224	A three-step mass spectrometry fragmentation strategy was used for glycopeptide identification, facilitated by pGlyco software annotation and manual checking.					
29405331	1	83	theme	mass	103:106	arg1	spectrometry					108:119	liquid chromatography-tandem mass spectrometry	74:119	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology	72:148	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	1	83	theme	mass	103:106	arg1	LC-MS/MS					122:129	LC-MS/MS	122:129	LC-MS/MS	122:129	A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methodology has been developed to differentiate core- and antennary-fucosylated glycosylation of glycopeptides.					
29405331	10	84	with	bi-	1613:1615	arg1	core-					1661:1665	core-	1661:1665	core-	1661:1665	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	84	with	bi-	1613:1615	arg1	antennary-fucosylation					1671:1692	antennary-fucosylation	1671:1692	antennary-fucosylation	1671:1692	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	0	85	theme	alpha-1-antitrypsin	16:34	arg1	glycosylation					36:48	alpha-1-antitrypsin glycosylation	16:48	alpha-1-antitrypsin glycosylation with direct LC-MS/MS	16:69	The analysis of alpha-1-antitrypsin glycosylation with direct LC-MS/MS.					
29405331	10	86	with	tri-	1618:1621	arg1	core-					1661:1665	core-	1661:1665	core-	1661:1665	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	86	with	tri-	1618:1621	arg1	antennary-fucosylation					1671:1692	antennary-fucosylation	1671:1692	antennary-fucosylation	1671:1692	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	11	87	theme	A1AT	1886:1889	arg1	protein					1891:1897	the A1AT protein	1882:1897	the A1AT protein	1882:1897	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	10	88	theme	glycosylation	1451:1463	arg1	sites					1465:1469	all three glycosylation sites	1441:1469	all three glycosylation sites of A1AT glycopeptides	1441:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	88	theme	glycosylation	1451:1463	arg1	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	3	89	used	used	535:538	arg2	A1AT					462:465	A1AT	462:465	A1AT	462:465	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	3	89	used	used	535:538	arg2	glycoprotein					428:439	The serum glycoprotein alpha-1-antitrypsin	418:459	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites	418:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	9	90	theme	glycopeptide	1265:1276	arg1	fragmentation					1278:1290	initial glycopeptide fragmentation	1257:1290	initial glycopeptide fragmentation	1257:1290	The collision energy used for initial glycopeptide fragmentation was found to be crucial for improved detection of oxonium ions and better selection of Y1 ion (peptide+GlcNAc).					
29405331	3	91	theme	antennary-fucosylated	498:518	arg1	glycosites					520:529	antennary-fucosylated glycosites	498:529	antennary-fucosylated glycosites	498:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	10	92	with	bi-	1716:1718	arg1	antennary-fucosylation					1766:1787	antennary-fucosylation	1766:1787	antennary-fucosylation	1766:1787	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	92	with	bi-	1716:1718	arg1	core-					1756:1760	core-	1756:1760	core-	1756:1760	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	4	93	theme	sialic	588:593	arg1	acids					595:599	the sialic acids	584:599	the sialic acids	584:599	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	4	94	with	glycopeptides	691:703	arg1	structures					725:734	similar glycan structures	710:734	similar glycan structures	710:734	Sialidase was used to remove the sialic acids in order to simplify the glycosylation microheterogeneity and to enhance the MS signal of glycopeptides with similar glycan structures.					
29405331	10	95	theme	glycopeptides	1479:1491	arg1	sites					1465:1469	all three glycosylation sites	1441:1469	all three glycosylation sites of A1AT glycopeptides	1441:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	95	theme	glycopeptides	1479:1491	arg1	glycopeptides					1479:1491	A1AT glycopeptides	1474:1491	A1AT glycopeptides	1474:1491	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	6	96	theme	abundance	934:942	arg1	modification					951:962	low abundance glycan modification	930:962	low abundance glycan modification	930:962	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	6	97	theme	In-source	818:826	arg1	dissociation					828:839	In-source dissociation	818:839	In-source dissociation	818:839	In-source dissociation was found to severely affect the identification and quantification of glycopeptides with low abundance glycan modification.					
29405331	10	98	contain	contains	1541:1548	arg2	glycans					1562:1568	biantennary glycans	1550:1568	biantennary glycans	1550:1568	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	10	98	contain	contains	1541:1548	arg1	site					1530:1533	site Asn70	1530:1539	site Asn70	1530:1539	Structural assignments revealed that all three glycosylation sites of A1AT glycopeptides contain complex N-glycan structures: site Asn70 contains biantennary glycans without fucosylation; site Asn107 contains bi-, tri- and tetra-antennary glycans with both core- and antennary-fucosylation; site Asn271 contains bi- and tri-antennary glycans with both core- and antennary-fucosylation.					
29405331	3	99	gly	glycosites	520:529	arg2	glycosites					520:529	antennary-fucosylated glycosites	498:529	antennary-fucosylated glycosites	498:529	The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study.					
29405331	11	100	theme	relative	1794:1801	arg1	intensity					1803:1811	The relative intensity	1790:1811	The relative intensity of core- and antennary-fucosylation on Asn107	1790:1857	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	11	100	theme	relative	1794:1801	arg1	similar					1863:1869	similar	1863:1869	similar	1863:1869	The relative intensity of core- and antennary-fucosylation on Asn107 was similar to that of the A1AT protein indicating that the glycosylation level of Asn107 is much larger than the other two sites.					
29405331	2	101	theme	glycopeptide	395:406	arg1	analysis					408:415	intact glycopeptide analysis	388:415	intact glycopeptide analysis	388:415	Both the glycosylation sites (heterogeneity) and multiple possible glycan occupancy at each site (microheterogeneity) can be resolved via intact glycopeptide analysis.					
29405331	7	102	theme	mass	985:988	arg1	spectrometer					990:1001	the mass spectrometer	981:1001	the mass spectrometer	981:1001	The settings of the mass spectrometer were therefore optimized to minimize the in-source dissociation.					
30134158	0	0	theme	Trimeric	81:88	arg1	Immunogen					90:98	a Soluble Trimeric Immunogen	71:98	a Soluble Trimeric Immunogen	71:98	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	7	1	theme	manufacturing	1101:1113	arg1	programs					1115:1122	manufacturing programs	1101:1122	manufacturing programs	1101:1122	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	6	2	theme	key	845:847	arg1	epitopes					854:861	key bnAb epitopes	845:861	key bnAb epitopes	845:861	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	4	3	theme	lymphoid	650:657	arg1	line					664:667	a lymphoid cell line	648:667	a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	648:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	0	4	theme	Soluble	73:79	arg1	Immunogen					90:98	a Soluble Trimeric Immunogen	71:98	a Soluble Trimeric Immunogen	71:98	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	4	5	with	line	664:667	arg1	those					674:678	those	674:678	those	674:678	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	6	6	contain	contain	966:972	arg1	latter					956:961	latter	956:961	latter	956:961	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	6	6	contain	contain	966:972	arg2	hotspots					974:981	hotspots	974:981	hotspots of elevated glycan processing	974:1011	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	4	7	from	glycosylation	566:578	arg1	virions					628:634	BG505 (clade A) virions	612:634	BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	612:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	8	theme	BG505	697:701	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	8	theme	BG505	697:701	arg1	trimers					709:715	recombinant BG505 SOSIP trimers	685:715	recombinant BG505 SOSIP trimers	685:715	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	3	9	from	ones	496:499	arg1	viruses					515:521	infectious viruses	504:521	infectious viruses	504:521	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	4	10	from	virions	628:634	arg1	subunits					598:605	gp120 and gp41 subunits	583:605	gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	583:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	10	from	virions	628:634	arg1	glycosylation					566:578	the overall glycosylation	554:578	the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	554:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	11	theme	overall	558:564	arg1	glycosylation					566:578	the overall glycosylation	554:578	the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	554:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	12	link	CHO-derived	728:738	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	1	13	gly	glycoprotein	243:254	arg1	glycoprotein					243:254	envelope glycoprotein	234:254	native, virion-associated envelope glycoprotein (Env) spikes	208:267	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	13	gly	glycoprotein	243:254	arg1	Env					257:259	Env	257:259	Env	257:259	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	2	14	theme	same	274:277	arg1	bnAbs					279:283	The same bnAbs	270:283	The same bnAbs	270:283	The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design.					
30134158	6	15	theme	bnAb	849:852	arg1	epitopes					854:861	key bnAb epitopes	845:861	key bnAb epitopes	845:861	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	3	16	theme	structural	396:405	arg1	trimers					378:384	SOSIP trimers	372:384	SOSIP trimers	372:384	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	16	theme	structural	396:405	arg1	mimics					421:426	close structural and antigenic mimics	390:426	close structural and antigenic mimics of virion Env	390:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	7	17	theme	Env	1053:1055	arg1	glycosylation					1057:1069	native versus recombinant Env glycosylation	1027:1069	native versus recombinant Env glycosylation	1027:1069	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	6	18	theme	elevated	986:993	arg1	processing					1002:1011	elevated glycan processing	986:1011	elevated glycan processing	986:1011	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	3	19	theme	virion	431:436	arg1	Env					438:440	virion Env	431:440	virion Env	431:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	20	theme	SOSIP	372:376	arg1	trimers					378:384	SOSIP trimers	372:384	SOSIP trimers	372:384	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	20	theme	SOSIP	372:376	arg1	mimics					421:426	close structural and antigenic mimics	390:426	close structural and antigenic mimics of virion Env	390:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	6	21	theme	Env	929:931	arg1	proteins					933:940	virion-derived Env proteins	914:940	virion-derived Env proteins	914:940	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	7	22	theme	glycosylation	1057:1069	arg1	Knowledge					1014:1022	Knowledge	1014:1022	Knowledge of native versus recombinant Env glycosylation	1014:1069	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	4	23	theme	recombinant	685:695	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	23	theme	recombinant	685:695	arg1	trimers					709:715	recombinant BG505 SOSIP trimers	685:715	recombinant BG505 SOSIP trimers	685:715	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	6	24	theme	glycan	995:1000	arg1	processing					1002:1011	elevated glycan processing	986:1011	elevated glycan processing	986:1011	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	3	25	theme	antigenic	411:419	arg1	trimers					378:384	SOSIP trimers	372:384	SOSIP trimers	372:384	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	25	theme	antigenic	411:419	arg1	mimics					421:426	close structural and antigenic mimics	390:426	close structural and antigenic mimics of virion Env	390:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	0	26	theme	Site-Specific	0:12	arg1	Glycosylation					14:26	Site-Specific Glycosylation	0:26	Site-Specific Glycosylation of Virion-Derived HIV-1 Env	0:54	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	2	27	theme	recombinant	298:308	arg1	immunogens					328:337	recombinant, soluble trimeric immunogens	298:337	recombinant, soluble trimeric immunogens based on the SOSIP design	298:363	The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design.					
30134158	6	28	theme	relevant	833:840	arg1	Glycans					825:831	Glycans	825:831	Glycans relevant to key bnAb epitopes	825:861	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	0	29	theme	Virion-Derived	31:44	arg1	Env					52:54	Virion-Derived HIV-1 Env	31:54	Virion-Derived HIV-1 Env	31:54	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	7	30	theme	recombinant	1041:1051	arg1	glycosylation					1057:1069	native versus recombinant Env glycosylation	1027:1069	native versus recombinant Env glycosylation	1027:1069	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	4	31	gly	glycosylation	566:578	arg1	subunits					598:605	gp120 and gp41 subunits	583:605	gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	583:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	1	32	theme	Many	101:104	arg1	bnAbs					139:143	bnAbs	139:143	bnAbs	139:143	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	32	theme	Many	101:104	arg1	antibodies					127:136	Many broadly neutralizing antibodies	101:136	Many broadly neutralizing antibodies (bnAbs) against HIV-1	101:158	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	5	33	theme	site-specific	792:804	arg1	analyses					806:813	detailed site-specific analyses	783:813	detailed site-specific analyses of gp120	783:822	We also performed detailed site-specific analyses of gp120.					
30134158	5	34	theme	detailed	783:790	arg1	analyses					806:813	detailed site-specific analyses	783:813	detailed site-specific analyses of gp120	783:822	We also performed detailed site-specific analyses of gp120.					
30134158	7	35	theme	native	1027:1032	arg1	glycosylation					1057:1069	native versus recombinant Env glycosylation	1027:1069	native versus recombinant Env glycosylation	1027:1069	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	3	36	theme	infectious	504:513	arg1	viruses					515:521	infectious viruses	504:521	infectious viruses	504:521	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	1	37	theme	broadly	106:112	arg1	bnAbs					139:143	bnAbs	139:143	bnAbs	139:143	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	37	theme	broadly	106:112	arg1	antibodies					127:136	Many broadly neutralizing antibodies	101:136	Many broadly neutralizing antibodies (bnAbs) against HIV-1	101:158	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	4	38	theme	subunits	598:605	arg1	glycosylation					566:578	the overall glycosylation	554:578	the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	554:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	0	39	theme	Env	52:54	arg1	Glycosylation					14:26	Site-Specific Glycosylation	0:26	Site-Specific Glycosylation of Virion-Derived HIV-1 Env	0:54	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	4	40	theme	cell	659:662	arg1	line					664:667	a lymphoid cell line	648:667	a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	648:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	41	theme	gp41	593:596	arg1	subunits					598:605	gp120 and gp41 subunits	583:605	gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	583:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	3	42	theme	Env	438:440	arg1	trimers					378:384	SOSIP trimers	372:384	SOSIP trimers	372:384	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	42	theme	Env	438:440	arg1	mimics					421:426	close structural and antigenic mimics	390:426	close structural and antigenic mimics of virion Env	390:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	1	43	theme	native	208:213	arg1	spikes					262:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	0	44	theme	HIV-1	46:50	arg1	Env					52:54	Virion-Derived HIV-1 Env	31:54	Virion-Derived HIV-1 Env	31:54	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	6	45	theme	virion-derived	914:927	arg1	proteins					933:940	virion-derived Env proteins	914:940	virion-derived Env proteins	914:940	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	0	46	gly	Glycosylation	14:26	arg1	Env					52:54	Virion-Derived HIV-1 Env	31:54	Virion-Derived HIV-1 Env	31:54	Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen.					
30134158	4	47	theme	BG505	612:616	arg1	virions					628:634	BG505 (clade A) virions	612:634	BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	612:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	6	48	theme	processing	1002:1011	arg1	hotspots					974:981	hotspots	974:981	hotspots of elevated glycan processing	974:1011	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	1	49	theme	neutralizing	114:125	arg1	bnAbs					139:143	bnAbs	139:143	bnAbs	139:143	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	49	theme	neutralizing	114:125	arg1	antibodies					127:136	Many broadly neutralizing antibodies	101:136	Many broadly neutralizing antibodies (bnAbs) against HIV-1	101:158	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	3	50	theme	close	390:394	arg1	trimers					378:384	SOSIP trimers	372:384	SOSIP trimers	372:384	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	3	50	theme	close	390:394	arg1	mimics					421:426	close structural and antigenic mimics	390:426	close structural and antigenic mimics of virion Env	390:440	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	7	51	theme	vaccine	1082:1088	arg1	design					1090:1095	vaccine design	1082:1095	vaccine design	1082:1095	Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.					
30134158	3	52	theme	glycan	469:474	arg1	structures					476:485	their glycan structures	463:485	their glycan structures	463:485	While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined.					
30134158	6	53	theme	recombinant	892:902	arg1	SOSIP					904:908	the recombinant SOSIP	888:908	the recombinant SOSIP	888:908	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	4	54	theme	grade	749:753	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	1	55	dep	native	208:213	arg1	virion-associated					216:232	virion-associated	216:232	virion-associated	216:232	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	56	dep	glycan	191:196	arg1	shield					198:203	shield	198:203	shield	198:203	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	2	57	theme	trimeric	319:326	arg1	immunogens					328:337	recombinant, soluble trimeric immunogens	298:337	recombinant, soluble trimeric immunogens based on the SOSIP design	298:363	The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design.					
30134158	4	58	theme	SOSIP	703:707	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	58	theme	SOSIP	703:707	arg1	trimers					709:715	recombinant BG505 SOSIP trimers	685:715	recombinant BG505 SOSIP trimers	685:715	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	59	theme	gp120	583:587	arg1	subunits					598:605	gp120 and gp41 subunits	583:605	gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	583:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	60	theme	CHO-derived	728:738	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	61	theme	clinical	740:747	arg1	material					755:762	CHO-derived clinical grade material	728:762	CHO-derived clinical grade material	728:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	2	62	dep	recombinant	298:308	arg1	soluble					311:317	soluble	311:317	soluble	311:317	The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design.					
30134158	1	63	theme	envelope	234:241	arg1	glycoprotein					243:254	envelope glycoprotein	234:254	native, virion-associated envelope glycoprotein (Env) spikes	208:267	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	1	63	theme	envelope	234:241	arg1	Env					257:259	Env	257:259	Env	257:259	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	5	64	theme	gp120	818:822	arg1	analyses					806:813	detailed site-specific analyses	783:813	detailed site-specific analyses of gp120	783:822	We also performed detailed site-specific analyses of gp120.					
30134158	6	65	link	virion-derived	914:927	arg1	proteins					933:940	virion-derived Env proteins	914:940	virion-derived Env proteins	914:940	Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing.					
30134158	1	66	from	glycan	191:196	arg1	spikes					262:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	4	67	theme	clade	619:623	arg1	BG505					612:616	BG505	612:616	BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material	612:762	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	4	67	theme	clade	619:623	arg1	A					625:625	clade A	619:625	clade A	619:625	Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material.					
30134158	1	68	theme	glycoprotein	243:254	arg1	spikes					262:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	native, virion-associated envelope glycoprotein (Env) spikes	208:267	Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes.					
30134158	2	69	theme	SOSIP	352:356	arg1	design					358:363	the SOSIP design	348:363	the SOSIP design	348:363	The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design.					
29420040	0	0	theme	Key	94:96	arg1	Lineages					105:112	Key B-Cell Lineages	94:112	Key B-Cell Lineages	94:112	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	1	1	theme	germline	323:330	arg1	precursors					332:341	the predicted human germline precursors	303:341	the predicted human germline precursors of bNAbs (gl-bNAbs)	303:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	4	2	theme	bNAb	850:853	arg1	epitopes					855:862	multiple bNAb epitopes	841:862	multiple bNAb epitopes	841:862	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	5	3	theme	N-glycan	1062:1069	arg1	analysis					1089:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	6	4	from	increases	1208:1216	arg1	extent					1225:1230	the extent	1221:1230	the extent of processing	1221:1244	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	5	5	theme	potential	920:928	arg1	sites					939:943	15 potential N-glycan sites	917:943	15 potential N-glycan sites (5 per protomer)	917:960	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	4	6	theme	multiple	841:848	arg1	epitopes					855:862	multiple bNAb epitopes	841:862	multiple bNAb epitopes	841:862	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	2	7	theme	shielding	497:505	arg1	glycans					507:513	these shielding glycans	491:513	these shielding glycans	491:513	The protein components of gl-bNAb epitopes are often masked by glycans, while mature bNAbs can evolve to accommodate or bypass these shielding glycans.					
29420040	7	8	theme	glycan	1433:1438	arg1	integrity					1416:1424	the overall integrity	1404:1424	the overall integrity of the glycan shield	1404:1445	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	1	9	gly	glycoprotein	195:206	arg1	glycoprotein					195:206	the trimeric HIV-1 envelope glycoprotein spike	167:212	the trimeric HIV-1 envelope glycoprotein spike (Env)	167:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	9	gly	glycoprotein	195:206	arg1	Env					215:217	Env	215:217	Env	215:217	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	5	10	theme	N-glycan	930:937	arg1	sites					939:943	15 potential N-glycan sites	917:943	15 potential N-glycan sites (5 per protomer)	917:960	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	3	11	theme	germline-targeting	530:547	arg1	immunogens					553:562	germline-targeting Env immunogens	530:562	germline-targeting Env immunogens	530:562	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	0	12	theme	B-Cell	98:103	arg1	Lineages					105:112	Key B-Cell Lineages	94:112	Key B-Cell Lineages	94:112	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	1	13	theme	bNAbs	346:350	arg1	precursors					332:341	the predicted human germline precursors	303:341	the predicted human germline precursors of bNAbs (gl-bNAbs)	303:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	5	14	theme	sites	939:943	arg1	removal					906:912	the removal	902:912	the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1,	902:1026	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	15	theme	mass	1071:1074	arg1	analysis					1089:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	7	16	theme	BG505	1365:1369	arg1	trimers					1377:1383	BG505 SOSIP trimers	1365:1383	BG505 SOSIP trimers	1365:1383	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	7	17	theme	SOSIP	1371:1375	arg1	trimers					1377:1383	BG505 SOSIP trimers	1365:1383	BG505 SOSIP trimers	1365:1383	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	4	18	theme	glycans	658:664	arg1	processing					644:653	the processing	640:653	the processing of glycans on Env trimers	640:679	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	1	19	theme	trimeric	171:178	arg1	glycoprotein					195:206	the trimeric HIV-1 envelope glycoprotein spike	167:212	the trimeric HIV-1 envelope glycoprotein spike (Env)	167:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	19	theme	trimeric	171:178	arg1	Env					215:217	Env	215:217	Env	215:217	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	5	20	theme	spectrometry	1076:1087	arg1	analysis					1089:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	4	21	theme	under-processed	809:823	arg1	glycans					825:831	under-processed glycans	809:831	under-processed glycans	809:831	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	5	22	theme	removal	906:912	arg1	impact					892:897	the impact	888:897	the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1,	888:1026	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	4	23	from	processing	644:653	arg1	trimers					673:679	Env trimers	669:679	Env trimers	669:679	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	1	24	theme	HIV-1	180:184	arg1	glycoprotein					195:206	the trimeric HIV-1 envelope glycoprotein spike	167:212	the trimeric HIV-1 envelope glycoprotein spike (Env)	167:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	24	theme	HIV-1	180:184	arg1	Env					215:217	Env	215:217	Env	215:217	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	7	25	theme	overall	1408:1414	arg1	integrity					1416:1424	the overall integrity	1404:1424	the overall integrity of the glycan shield	1404:1445	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	3	26	theme	Env	549:551	arg1	immunogens					553:562	germline-targeting Env immunogens	530:562	germline-targeting Env immunogens	530:562	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	1	27	theme	envelope	186:193	arg1	glycoprotein					195:206	the trimeric HIV-1 envelope glycoprotein spike	167:212	the trimeric HIV-1 envelope glycoprotein spike (Env)	167:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	27	theme	envelope	186:193	arg1	Env					215:217	Env	215:217	Env	215:217	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	28	theme	recombinant	259:269	arg1	proteins					275:282	recombinant Env proteins	259:282	recombinant Env proteins	259:282	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	6	29	theme	subtle	1201:1206	arg1	increases					1208:1216	subtle increases	1201:1216	little overall change in the glycan profile but only subtle increases in the extent of processing	1148:1244	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	3	30	theme	immunogens	553:562	arg1	design					520:525	The design	516:525	The design of germline-targeting Env immunogens	516:562	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	6	31	theme	processing	1235:1244	arg1	extent					1225:1230	the extent	1221:1230	the extent of processing	1221:1244	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	0	32	theme	Processing	27:36	arg1	Integrity					0:8	Integrity	0:8	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen	0:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	1	33	theme	Env	271:273	arg1	proteins					275:282	recombinant Env proteins	259:282	recombinant Env proteins	259:282	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	5	34	from	impact	892:897	arg1	SOSIP.v4.1-GT1					1012:1025	BG505 SOSIP.v4.1-GT1	1006:1025	BG505 SOSIP.v4.1-GT1	1006:1025	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	34	from	impact	892:897	arg1	trimer					998:1003	the germline-targeting soluble trimer	967:1003	the germline-targeting soluble trimer	967:1003	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	35	from	trimer	998:1003	arg1	impact					892:897	the impact	888:897	the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1,	888:1026	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	35	from	trimer	998:1003	arg1	removal					906:912	the removal	902:912	the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1,	902:1026	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	1	36	theme	spike	208:212	arg1	glycoprotein					195:206	the trimeric HIV-1 envelope glycoprotein spike	167:212	the trimeric HIV-1 envelope glycoprotein spike (Env)	167:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	36	theme	spike	208:212	arg1	Env					215:217	Env	215:217	Env	215:217	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	0	37	theme	Glycosylation	13:25	arg1	Processing					27:36	Glycosylation Processing	13:36	Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen	13:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	1	38	theme	proteins	275:282	arg1	design					249:254	the design	245:254	the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs)	245:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	6	39	theme	little	1148:1153	arg1	change					1163:1168	little overall change	1148:1168	little overall change in the glycan profile but only subtle increases in the extent of processing	1148:1244	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	1	40	theme	Broadly	115:121	arg1	bNAbs					148:152	bNAbs	148:152	bNAbs	148:152	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	40	theme	Broadly	115:121	arg1	antibodies					136:145	Broadly neutralizing antibodies	115:145	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env)	115:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	40	theme	Broadly	115:121	arg1	tools					224:228	tools	224:228	tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs)	224:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	2	41	theme	epitopes	398:405	arg1	components					376:385	The protein components	364:385	The protein components of gl-bNAb epitopes	364:405	The protein components of gl-bNAb epitopes are often masked by glycans, while mature bNAbs can evolve to accommodate or bypass these shielding glycans.					
29420040	5	42	theme	quantitative	1034:1045	arg1	analysis					1089:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	quantitative, site-specific N-glycan mass spectrometry analysis	1034:1096	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	6	43	theme	overall	1155:1161	arg1	change					1163:1168	little overall change	1148:1168	little overall change in the glycan profile but only subtle increases in the extent of processing	1148:1244	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	2	44	theme	gl-bNAb	390:396	arg1	epitopes					398:405	gl-bNAb epitopes	390:405	gl-bNAb epitopes	390:405	The protein components of gl-bNAb epitopes are often masked by glycans, while mature bNAbs can evolve to accommodate or bypass these shielding glycans.					
29420040	2	45	theme	mature	442:447	arg1	bNAbs					449:453	mature bNAbs	442:453	mature bNAbs	442:453	The protein components of gl-bNAb epitopes are often masked by glycans, while mature bNAbs can evolve to accommodate or bypass these shielding glycans.					
29420040	3	46	theme	sites	624:628	arg1	deletion					596:603	the targeted deletion	583:603	the targeted deletion of specific glycan sites	583:628	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	3	47	theme	targeted	587:594	arg1	deletion					596:603	the targeted deletion	583:603	the targeted deletion of specific glycan sites	583:628	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	7	48	theme	multiple	1328:1335	arg1	glycans					1337:1343	multiple glycans	1328:1343	multiple glycans	1328:1343	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	0	49	theme	Trimeric	59:66	arg1	Immunogen					74:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	7	50	dep	glycan	1433:1438	arg1	shield					1440:1445	shield	1440:1445	shield	1440:1445	We conclude that multiple glycans can be deleted from BG505 SOSIP trimers without perturbing the overall integrity of the glycan shield.					
29420040	5	51	theme	soluble	990:996	arg1	SOSIP.v4.1-GT1					1012:1025	BG505 SOSIP.v4.1-GT1	1006:1025	BG505 SOSIP.v4.1-GT1	1006:1025	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	51	theme	soluble	990:996	arg1	trimer					998:1003	the germline-targeting soluble trimer	967:1003	the germline-targeting soluble trimer	967:1003	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	1	52	theme	neutralizing	123:134	arg1	bNAbs					148:152	bNAbs	148:152	bNAbs	148:152	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	52	theme	neutralizing	123:134	arg1	antibodies					136:145	Broadly neutralizing antibodies	115:145	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env)	115:218	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	1	52	theme	neutralizing	123:134	arg1	tools					224:228	tools	224:228	tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs)	224:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	0	53	theme	Glycan-Depleted	43:57	arg1	Immunogen					74:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	6	54	theme	adjacent	1267:1274	arg1	sites					1249:1253	sites	1249:1253	sites immediately adjacent to	1249:1277	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	4	55	theme	essential	785:793	arg1	contributions					795:807	the essential contributions	781:807	the essential contributions under-processed glycans make to multiple bNAb epitopes	781:862	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	6	56	theme	glycan	1177:1182	arg1	profile					1184:1190	the glycan profile	1173:1190	the glycan profile	1173:1190	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	1	57	theme	predicted	307:315	arg1	precursors					332:341	the predicted human germline precursors	303:341	the predicted human germline precursors of bNAbs (gl-bNAbs)	303:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	3	58	theme	specific	608:615	arg1	sites					624:628	specific glycan sites	608:628	specific glycan sites	608:628	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	5	59	theme	germline-targeting	971:988	arg1	SOSIP.v4.1-GT1					1012:1025	BG505 SOSIP.v4.1-GT1	1006:1025	BG505 SOSIP.v4.1-GT1	1006:1025	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	5	59	theme	germline-targeting	971:988	arg1	trimer					998:1003	the germline-targeting soluble trimer	967:1003	the germline-targeting soluble trimer	967:1003	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	3	60	theme	glycan	617:622	arg1	sites					624:628	specific glycan sites	608:628	specific glycan sites	608:628	The design of germline-targeting Env immunogens therefore includes the targeted deletion of specific glycan sites.					
29420040	5	61	dep	sites	939:943	arg1	5					946:946	5	946:946	5	946:946	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
29420040	0	62	theme	Immunogen	74:82	arg1	Processing					27:36	Glycosylation Processing	13:36	Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen	13:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	4	63	dep	contributions	795:807	arg1	make					833:836	make	833:836	make to multiple bNAb epitopes	833:862	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	4	64	theme	Env	669:671	arg1	trimers					673:679	Env trimers	669:679	Env trimers	669:679	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	2	65	theme	protein	368:374	arg1	components					376:385	The protein components	364:385	The protein components of gl-bNAb epitopes	364:405	The protein components of gl-bNAb epitopes are often masked by glycans, while mature bNAbs can evolve to accommodate or bypass these shielding glycans.					
29420040	0	66	theme	HIV-1	68:72	arg1	Immunogen					74:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	a Glycan-Depleted Trimeric HIV-1 Immunogen	41:82	Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.					
29420040	1	67	theme	human	317:321	arg1	precursors					332:341	the predicted human germline precursors	303:341	the predicted human germline precursors of bNAbs (gl-bNAbs)	303:361	Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs).					
29420040	4	68	theme	relevant	760:767	arg1	density					706:712	the density	702:712	the density with which they are packed together	702:748	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	4	68	theme	relevant	760:767	arg1	point					769:773	a highly relevant point	751:773	a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes	751:862	However, the processing of glycans on Env trimers can be influenced by the density with which they are packed together, a highly relevant point given the essential contributions under-processed glycans make to multiple bNAb epitopes.					
29420040	6	69	from	change	1163:1168	arg1	profile					1184:1190	the glycan profile	1173:1190	the glycan profile	1173:1190	We find that, compared with SOSIP.664, there was little overall change in the glycan profile but only subtle increases in the extent of processing at sites immediately adjacent to where glycans had been deleted.					
29420040	5	70	dep	quantitative	1034:1045	arg1	site-specific					1048:1060	site-specific	1048:1060	site-specific	1048:1060	We sought to determine the impact of the removal of 15 potential N-glycan sites (5 per protomer) from the germline-targeting soluble trimer, BG505 SOSIP.v4.1-GT1, using quantitative, site-specific N-glycan mass spectrometry analysis.					
30084397	4	0	theme	crystal	923:929	arg1	packing					931:937	the corresponding crystal packing	905:937	the corresponding crystal packing	905:937	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	6	1	contain	contains	1178:1185	arg1	structure					1168:1176	The final HLE structure	1154:1176	The final HLE structure	1154:1176	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	6	1	contain	contains	1178:1185	arg2	trees					1233:1237	the largest structurally defined carbohydrate trees	1187:1237	the largest structurally defined carbohydrate trees among currently available HLE structures	1187:1278	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	6	2	theme	available	1255:1263	arg1	structures					1269:1278	currently available HLE structures	1245:1278	currently available HLE structures	1245:1278	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	2	3	theme	scissile	672:679	arg1	bond					689:692	the scissile peptide bond	668:692	the scissile peptide bond in a substrate protein	668:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	4	theme	HLE	361:363	arg1	structures					365:374	previously described HLE structures	340:374	previously described HLE structures	340:374	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	5	theme	substrate-recognition	471:491	arg1	sites					493:497	the S1 and S2 substrate-recognition sites	457:497	the S1 and S2 substrate-recognition sites	457:497	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	0	6	from	structure	8:16	arg1	complex					69:75	complex	69:75	complex with an S2' site binding inhibitor	69:110	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	2	7	theme	S2	580:581	arg1	site					584:587	the S2' site	576:587	the S2' site	576:587	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	8	theme	second	624:629	arg1	chain					636:640	the second side chain	620:640	the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	620:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	9	theme	described	351:359	arg1	structures					365:374	previously described HLE structures	340:374	previously described HLE structures	340:374	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	5	10	theme	crystalline	1005:1015	arg1	environment					1017:1027	This novel crystalline environment	994:1027	This novel crystalline environment	994:1027	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	4	11	theme	such	885:888	arg1	dimers					894:899	such HLE dimers	885:899	such HLE dimers	885:899	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	2	12	theme	S2	468:469	arg1	sites					493:497	the S1 and S2 substrate-recognition sites	457:497	the S1 and S2 substrate-recognition sites	457:497	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	3	13	theme	HLE	774:776	arg1	dimers					778:783	crystalline HLE dimers	762:783	crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution	762:874	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	0	14	theme	S2	85:86	arg1	inhibitor					102:110	an S2' site binding inhibitor	82:110	an S2' site binding inhibitor	82:110	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	0	15	with	complex	69:75	arg1	inhibitor					102:110	an S2' site binding inhibitor	82:110	an S2' site binding inhibitor	82:110	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	3	16	theme	dimers	778:783	arg1	formation					749:757	the formation	745:757	the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution	745:874	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	0	17	theme	binding	94:100	arg1	inhibitor					102:110	an S2' site binding inhibitor	82:110	an S2' site binding inhibitor	82:110	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	6	18	theme	carbohydrate	1220:1231	arg1	trees					1233:1237	the largest structurally defined carbohydrate trees	1187:1237	the largest structurally defined carbohydrate trees among currently available HLE structures	1187:1278	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	2	19	from	side	660:663	arg1	protein					709:715	a substrate protein	697:715	a substrate protein	697:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	19	from	side	660:663	arg1	chain					636:640	the second side chain	620:640	the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	620:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	0	20	theme	site	89:92	arg1	inhibitor					102:110	an S2' site binding inhibitor	82:110	an S2' site binding inhibitor	82:110	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	1	21	from	inhibitor	291:299	arg1	studies					316:322	preliminary studies	304:322	preliminary studies	304:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	2	22	theme	substrate	699:707	arg1	protein					709:715	a substrate protein	697:715	a substrate protein	697:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	4	23	theme	previous	961:968	arg1	structures					982:991	previous HLE crystal structures	961:991	previous HLE crystal structures	961:991	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	4	24	theme	corresponding	909:921	arg1	packing					931:937	the corresponding crystal packing	905:937	the corresponding crystal packing	905:937	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	5	25	theme	HLE	1117:1119	arg1	chains					1107:1112	the N-glycan chains	1094:1112	the N-glycan chains of HLE	1094:1119	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	0	26	theme	Crystal	0:6	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.	0:111	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	6	27	theme	largest	1191:1197	arg1	trees					1233:1237	the largest structurally defined carbohydrate trees	1187:1237	the largest structurally defined carbohydrate trees among currently available HLE structures	1187:1278	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	2	28	theme	small-molecule	381:394	arg1	inhibitors					396:405	small-molecule inhibitors	381:405	small-molecule inhibitors	381:405	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	4	29	theme	crystal	974:980	arg1	structures					982:991	previous HLE crystal structures	961:991	previous HLE crystal structures	961:991	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	2	30	theme	side	631:634	arg1	chain					636:640	the second side chain	620:640	the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	620:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	4	31	theme	HLE	970:972	arg1	structures					982:991	previous HLE crystal structures	961:991	previous HLE crystal structures	961:991	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	1	32	theme	HLE	287:289	arg1	inhibitor					291:299	an HLE inhibitor	284:299	an HLE inhibitor in preliminary studies	284:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	32	theme	HLE	287:289	arg1	derivative					245:254	a 1,3-thiazolidine-2,4-dione derivative	216:254	a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies	216:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	33	with	complex	203:209	arg1	inhibitor					291:299	an HLE inhibitor	284:299	an HLE inhibitor in preliminary studies	284:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	33	with	complex	203:209	arg1	derivative					245:254	a 1,3-thiazolidine-2,4-dione derivative	216:254	a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies	216:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	2	34	with	contrast	328:335	arg1	inhibitors					396:405	small-molecule inhibitors	381:405	small-molecule inhibitors	381:405	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	0	35	theme	human	41:45	arg1	elastase					57:64	highly glycosylated human leukocyte elastase	21:64	highly glycosylated human leukocyte elastase	21:64	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	5	36	theme	electron	1136:1143	arg1	density					1145:1151	electron density	1136:1151	electron density	1136:1151	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	2	37	theme	HLE	526:528	arg1	structure					530:538	the first HLE structure	516:538	the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	516:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	37	theme	HLE	526:528	arg1	this					508:511	this	508:511	this	508:511	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	3	38	from	stable	857:862	arg1	solution					867:874	solution	867:874	solution	867:874	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	0	39	theme	glycosylated	28:39	arg1	elastase					57:64	highly glycosylated human leukocyte elastase	21:64	highly glycosylated human leukocyte elastase	21:64	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	5	40	theme	N-glycan	1098:1105	arg1	chains					1107:1112	the N-glycan chains	1094:1112	the N-glycan chains of HLE	1094:1119	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	2	41	theme	first	520:524	arg1	structure					530:538	the first HLE structure	516:538	the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	516:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	41	theme	first	520:524	arg1	this					508:511	this	508:511	this	508:511	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	2	42	from	bond	689:692	arg1	protein					709:715	a substrate protein	697:715	a substrate protein	697:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	0	43	theme	elastase	57:64	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.	0:111	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	1	44	theme	preliminary	304:314	arg1	studies					316:322	preliminary studies	304:322	preliminary studies	304:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	6	45	dep	largest	1191:1197	arg1	defined					1212:1218	defined	1212:1218	defined	1212:1218	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	3	46	from	solution	867:874	arg1	stable					857:862	stable	857:862	stable	857:862	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	0	47	theme	leukocyte	47:55	arg1	elastase					57:64	highly glycosylated human leukocyte elastase	21:64	highly glycosylated human leukocyte elastase	21:64	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	2	48	from	protein	709:715	arg1	side					660:663	the C-terminal side	645:663	the C-terminal side of the scissile peptide bond in a substrate protein	645:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	6	49	theme	HLE	1164:1166	arg1	structure					1168:1176	The final HLE structure	1154:1176	The final HLE structure	1154:1176	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	4	50	located	observed	949:956	arg2	packing					931:937	the corresponding crystal packing	905:937	the corresponding crystal packing	905:937	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	4	50	located	observed	949:956	arg1	structures					982:991	previous HLE crystal structures	961:991	previous HLE crystal structures	961:991	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	4	50	located	observed	949:956	arg2	dimers					894:899	such HLE dimers	885:899	such HLE dimers	885:899	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	2	51	theme	synthetic	547:555	arg1	inhibitor					557:565	a synthetic inhibitor	545:565	a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	545:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	6	52	theme	final	1158:1162	arg1	structure					1168:1176	The final HLE structure	1154:1176	The final HLE structure	1154:1176	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	0	53	gly	glycosylated	28:39	arg1	elastase					57:64	highly glycosylated human leukocyte elastase	21:64	highly glycosylated human leukocyte elastase	21:64	Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.					
30084397	2	54	theme	C-terminal	649:658	arg1	side					660:663	the C-terminal side	645:663	the C-terminal side of the scissile peptide bond in a substrate protein	645:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	1	55	theme	Glycosylated	113:124	arg1	elastase					142:149	human leukocyte elastase	126:149	Glycosylated human leukocyte elastase (HLE)	113:155	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	55	theme	Glycosylated	113:124	arg1	HLE					152:154	HLE	152:154	HLE	152:154	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	3	56	theme	active	810:815	arg1	sites					817:821	the active sites	806:821	the active sites	806:821	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	6	57	theme	HLE	1265:1267	arg1	structures					1269:1278	currently available HLE structures	1245:1278	currently available HLE structures	1245:1278	The final HLE structure contains the largest structurally defined carbohydrate trees among currently available HLE structures.					
30084397	1	58	theme	human	126:130	arg1	elastase					142:149	human leukocyte elastase	126:149	Glycosylated human leukocyte elastase (HLE)	113:155	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	58	theme	human	126:130	arg1	HLE					152:154	HLE	152:154	HLE	152:154	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	3	59	theme	crystalline	762:772	arg1	dimers					778:783	crystalline HLE dimers	762:783	crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution	762:874	The inhibitor also induces the formation of crystalline HLE dimers that block access to the active sites and that are also predicted to be stable in solution.					
30084397	5	60	theme	novel	999:1003	arg1	environment					1017:1027	This novel crystalline environment	994:1027	This novel crystalline environment	994:1027	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	2	61	theme	bond	689:692	arg1	side					660:663	the C-terminal side	645:663	the C-terminal side of the scissile peptide bond in a substrate protein	645:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	1	62	theme	leukocyte	132:140	arg1	elastase					142:149	human leukocyte elastase	126:149	Glycosylated human leukocyte elastase (HLE)	113:155	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	62	theme	leukocyte	132:140	arg1	HLE					152:154	HLE	152:154	HLE	152:154	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	2	63	with	structure	530:538	arg1	inhibitor					557:565	a synthetic inhibitor	545:565	a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein	545:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	1	64	theme	1,3-thiazolidine-2,4-dione	218:243	arg1	inhibitor					291:299	an HLE inhibitor	284:299	an HLE inhibitor in preliminary studies	284:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	1	64	theme	1,3-thiazolidine-2,4-dione	218:243	arg1	derivative					245:254	a 1,3-thiazolidine-2,4-dione derivative	216:254	a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies	216:322	Glycosylated human leukocyte elastase (HLE) was crystallized and structurally analysed in complex with a 1,3-thiazolidine-2,4-dione derivative that had been identified as an HLE inhibitor in preliminary studies.					
30084397	2	65	theme	S1	461:462	arg1	sites					493:497	the S1 and S2 substrate-recognition sites	457:497	the S1 and S2 substrate-recognition sites	457:497	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	5	66	theme	large	1079:1083	arg1	parts					1085:1089	comparatively large parts	1065:1089	comparatively large parts of the N-glycan chains of HLE	1065:1119	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30084397	2	67	theme	peptide	681:687	arg1	bond					689:692	the scissile peptide bond	668:692	the scissile peptide bond in a substrate protein	668:715	In contrast to previously described HLE structures with small-molecule inhibitors, in this structure the inhibitor does not bind to the S1 and S2 substrate-recognition sites; rather, this is the first HLE structure with a synthetic inhibitor in which the S2' site is blocked that normally binds the second side chain at the C-terminal side of the scissile peptide bond in a substrate protein.					
30084397	4	68	theme	HLE	890:892	arg1	dimers					894:899	such HLE dimers	885:899	such HLE dimers	885:899	Neither such HLE dimers nor the corresponding crystal packing have been observed in previous HLE crystal structures.					
30084397	5	69	theme	chains	1107:1112	arg1	parts					1085:1089	comparatively large parts	1065:1089	comparatively large parts of the N-glycan chains of HLE	1065:1119	This novel crystalline environment contributes to the observation that comparatively large parts of the N-glycan chains of HLE are defined by electron density.					
30115684	4	0	with	technology	696:705	arg1	expression					730:739	recombinant gp120 expression	712:739	recombinant gp120 expression	712:739	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	7	1	theme	proteins	1503:1510	arg1	characterization					1430:1445	functional characterization	1419:1445	functional characterization of the gp120 glycans and potentially other glycosylated proteins	1419:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	5	2	theme	N-azidoacetylgalactosamine	897:922	arg1	incorporation					933:945	N-azidoacetylgalactosamine (GalNAz) incorporation	897:945	N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield	897:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	2	3	from	site	443:446	arg1	composition/structure					413:433	glycan composition/structure	406:433	glycan composition/structure	406:433	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	2	3	from	site	443:446	arg1	gp120					451:455	gp120	451:455	gp120	451:455	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	2	3	from	site	443:446	arg1	sites					396:400	Glycosylation sites	382:400	Glycosylation sites	382:400	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	1	4	theme	immense	225:231	arg1	attention					233:241	immense attention	225:241	immense attention	225:241	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	4	theme	immense	225:231	arg1	site					259:262	a vulnerable site	246:262	a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1	246:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	6	5	with	interaction	1136:1146	arg1	cells					1158:1162	host cells	1153:1162	host cells	1153:1162	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	1	6	gly	glycoprotein	154:165	arg1	glycoprotein					154:165	the envelope glycoprotein gp120	141:171	the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1)	141:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	7	theme	envelope	145:152	arg1	gp120					167:171	the envelope glycoprotein gp120	141:171	the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1)	141:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	0	8	theme	glycosylation	87:99	arg1	role					73:76	the role	69:76	the role of gp120 glycosylation on antigen uptake	69:117	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	3	9	theme	gp120	606:610	arg1	glycans					612:618	tracking gp120 glycans	597:618	directly and selectively tracking gp120 glycans	572:618	However, a method for directly and selectively tracking gp120 glycans has been lacking.					
30115684	6	10	theme	oligomannose	1187:1198	arg1	enrichment					1200:1209	oligomannose enrichment	1187:1209	oligomannose enrichment	1187:1209	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	6	11	theme	sialic	1215:1220	arg1	deficiency					1227:1236	sialic acid deficiency	1215:1236	sialic acid deficiency	1215:1236	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	0	12	theme	gp120	81:85	arg1	glycosylation					87:99	gp120 glycosylation	81:99	gp120 glycosylation	81:99	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	1	13	theme	significant	320:330	arg1	impact					332:337	its significant impact	316:337	its significant impact on host adaptive immune response to HIV-1	316:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	7	14	theme	other	1484:1488	arg1	proteins					1503:1510	potentially other glycosylated proteins	1472:1510	potentially other glycosylated proteins	1472:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	2	15	with	interactions	472:483	arg1	bNAbs					513:517	bNAbs	513:517	bNAbs	513:517	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	1	16	dep	glycan	124:129	arg1	shield					131:136	shield	131:136	shield	131:136	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	0	17	theme	antigen	104:110	arg1	uptake					112:117	antigen uptake	104:117	antigen uptake	104:117	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	4	18	with	labeling	667:674	arg1	expression					730:739	recombinant gp120 expression	712:739	recombinant gp120 expression	712:739	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	6	19	theme	glycosylation	1115:1127	arg1	impact					1099:1104	the impact	1095:1104	the impact of gp120 glycosylation on its interaction with host cells	1095:1162	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	2	20	theme	Glycosylation	382:394	arg1	sites					396:400	Glycosylation sites	382:400	Glycosylation sites	382:400	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	7	21	theme	glycans	1460:1466	arg1	characterization					1430:1445	functional characterization	1419:1445	functional characterization of the gp120 glycans and potentially other glycosylated proteins	1419:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	1	22	theme	glycoprotein	154:165	arg1	gp120					167:171	the envelope glycoprotein gp120	141:171	the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1)	141:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	7	23	theme	glycosylated	1490:1501	arg1	proteins					1503:1510	potentially other glycosylated proteins	1472:1510	potentially other glycosylated proteins	1472:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	5	24	dep	glycan	962:967	arg1	shield					969:974	shield	969:974	shield	969:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	4	25	theme	gp120	761:765	arg1	glycans					767:773	gp120 glycans	761:773	gp120 glycans	761:773	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	2	26	theme	gp120	488:492	arg1	interactions					472:483	the interactions	468:483	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs	382:517	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	1	27	theme	host	342:345	arg1	response					363:370	host adaptive immune response	342:370	host adaptive immune response to HIV-1	342:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	6	28	theme	dendritic	1295:1303	arg1	cells					1305:1309	bone marrow-derived dendritic cells	1275:1309	bone marrow-derived dendritic cells	1275:1309	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	1	29	from	glycan	124:129	arg1	gp120					167:171	the envelope glycoprotein gp120	141:171	the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1)	141:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	4	30	theme	gp120	724:728	arg1	expression					730:739	recombinant gp120 expression	712:739	recombinant gp120 expression	712:739	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	7	31	gly	glycosylated	1490:1501	arg1	proteins					1503:1510	potentially other glycosylated proteins	1472:1510	potentially other glycosylated proteins	1472:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	0	32	theme	Metabolic	0:8	arg1	labeling					10:17	Metabolic labeling	0:17	Metabolic labeling of HIV-1 envelope glycoprotein gp120	0:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	1	33	theme	vulnerable	248:257	arg1	attention					233:241	immense attention	225:241	immense attention	225:241	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	33	theme	vulnerable	248:257	arg1	site					259:262	a vulnerable site	246:262	a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1	246:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	6	34	theme	marrow-derived	1280:1293	arg1	cells					1305:1309	bone marrow-derived dendritic cells	1275:1309	bone marrow-derived dendritic cells	1275:1309	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	4	35	theme	recombinant	712:722	arg1	expression					730:739	recombinant gp120 expression	712:739	recombinant gp120 expression	712:739	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	1	36	theme	immune	356:361	arg1	response					363:370	host adaptive immune response	342:370	host adaptive immune response to HIV-1	342:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	0	37	theme	HIV-1	22:26	arg1	gp120					50:54	HIV-1 envelope glycoprotein gp120	22:54	HIV-1 envelope glycoprotein gp120	22:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	7	38	theme	gp120	1454:1458	arg1	glycans					1460:1466	the gp120 glycans	1450:1466	the gp120 glycans	1450:1466	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	6	39	from	impact	1099:1104	arg1	interaction					1136:1146	its interaction	1132:1146	its interaction with host cells	1132:1162	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	6	40	link	marrow-derived	1280:1293	arg1	cells					1305:1309	bone marrow-derived dendritic cells	1275:1309	bone marrow-derived dendritic cells	1275:1309	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	1	41	theme	immunodeficiency	182:197	arg1	virus					199:203	human immunodeficiency virus 1	176:205	human immunodeficiency virus 1 (HIV-1)	176:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	41	theme	immunodeficiency	182:197	arg1	HIV-1					208:212	HIV-1	208:212	HIV-1	208:212	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	42	from	impact	332:337	arg1	response					363:370	host adaptive immune response	342:370	host adaptive immune response to HIV-1	342:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	5	43	theme	gp120	956:960	arg1	glycan					962:967	the gp120 glycan shield	952:974	the gp120 glycan shield	952:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	1	44	theme	adaptive	347:354	arg1	response					363:370	host adaptive immune response	342:370	host adaptive immune response to HIV-1	342:379	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	6	45	theme	gp120	1109:1113	arg1	glycosylation					1115:1127	gp120 glycosylation	1109:1127	gp120 glycosylation	1109:1127	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	1	46	theme	virus	199:203	arg1	gp120					167:171	the envelope glycoprotein gp120	141:171	the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1)	141:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	0	47	theme	glycoprotein	37:48	arg1	gp120					50:54	HIV-1 envelope glycoprotein gp120	22:54	HIV-1 envelope glycoprotein gp120	22:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	6	48	theme	host	1153:1156	arg1	cells					1158:1162	host cells	1153:1162	host cells	1153:1162	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	0	49	from	role	73:76	arg1	uptake					112:117	antigen uptake	104:117	antigen uptake	104:117	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	6	50	theme	bone	1275:1278	arg1	cells					1305:1309	bone marrow-derived dendritic cells	1275:1309	bone marrow-derived dendritic cells	1275:1309	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	1	51	theme	human	176:180	arg1	virus					199:203	human immunodeficiency virus 1	176:205	human immunodeficiency virus 1 (HIV-1)	176:213	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	51	theme	human	176:180	arg1	HIV-1					208:212	HIV-1	208:212	HIV-1	208:212	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	0	52	theme	envelope	28:35	arg1	gp120					50:54	HIV-1 envelope glycoprotein gp120	22:54	HIV-1 envelope glycoprotein gp120	22:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	1	53	theme	neutralizing	276:287	arg1	antibodies					289:298	broadly neutralizing antibodies	268:298	broadly neutralizing antibodies (bNAbs)	268:306	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	1	53	theme	neutralizing	276:287	arg1	bNAbs					301:305	bNAbs	301:305	bNAbs	301:305	The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1.					
30115684	2	54	theme	glycan	406:411	arg1	composition/structure					413:433	glycan composition/structure	406:433	glycan composition/structure	406:433	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	5	55	theme	gp120	850:854	arg1	labeling					838:845	Selective labeling	828:845	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield	828:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	5	56	gly	glycopeptides	1026:1038	arg2	glycopeptides					1026:1038	tryptic glycopeptides	1018:1038	tryptic glycopeptides	1018:1038	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	4	57	theme	chemistry	686:694	arg1	technology					696:705	click chemistry technology	680:705	click chemistry technology	680:705	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	0	58	theme	gp120	50:54	arg1	labeling					10:17	Metabolic labeling	0:17	Metabolic labeling of HIV-1 envelope glycoprotein gp120	0:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	2	59	dep	along	457:461	arg1	with					463:466	with	463:466	with	463:466	Glycosylation sites and glycan composition/structure at each site on gp120 along with the interactions of gp120 glycan-shield with bNAbs have been extensively studied.					
30115684	6	60	theme	gp120	1259:1263	arg1	uptake					1265:1270	gp120 uptake	1259:1270	gp120 uptake	1259:1270	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	4	61	theme	click	680:684	arg1	technology					696:705	click chemistry technology	680:705	click chemistry technology	680:705	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	6	62	theme	acid	1222:1225	arg1	deficiency					1227:1236	sialic acid deficiency	1215:1236	sialic acid deficiency	1215:1236	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	3	63	theme	tracking	597:604	arg1	glycans					612:618	tracking gp120 glycans	597:618	directly and selectively tracking gp120 glycans	572:618	However, a method for directly and selectively tracking gp120 glycans has been lacking.					
30115684	7	64	theme	functional	1419:1428	arg1	characterization					1430:1445	functional characterization	1419:1445	functional characterization of the gp120 glycans and potentially other glycosylated proteins	1419:1510	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	5	65	theme	mass	997:1000	arg1	spectrometry					1002:1013	mass spectrometry	997:1013	mass spectrometry of tryptic glycopeptides	997:1038	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	5	66	theme	GalNAz	925:930	arg1	incorporation					933:945	N-azidoacetylgalactosamine (GalNAz) incorporation	897:945	N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield	897:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	0	67	gly	glycoprotein	37:48	arg1	glycoprotein					37:48	HIV-1 envelope glycoprotein gp120	22:54	HIV-1 envelope glycoprotein gp120	22:54	Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the role of gp120 glycosylation on antigen uptake.					
30115684	7	68	theme	detection	1369:1377	arg1	method					1379:1384	an effective labeling and detection method	1343:1384	method	1379:1384	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	6	69	theme	labeled	1064:1070	arg1	gp120					1072:1076	metabolically labeled gp120	1050:1076	metabolically labeled gp120	1050:1076	By using metabolically labeled gp120, we investigated the impact of gp120 glycosylation on its interaction with host cells, and demonstrated that oligomannose enrichment and sialic acid deficiency drastically enhanced gp120 uptake by bone marrow-derived dendritic cells.					
30115684	5	70	theme	tryptic	1018:1024	arg1	glycopeptides					1026:1038	tryptic glycopeptides	1018:1038	tryptic glycopeptides	1018:1038	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	5	71	theme	Selective	828:836	arg1	labeling					838:845	Selective labeling	828:845	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield	828:974	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
30115684	4	72	theme	metabolic	657:665	arg1	labeling					667:674	metabolic labeling	657:674	metabolic labeling	657:674	Here, we integrate metabolic labeling and click chemistry technology with recombinant gp120 expression to demonstrate that gp120 glycans could be specifically labeled and directly detected.					
30115684	7	73	theme	effective	1346:1354	arg1	labeling					1356:1363	an effective labeling and detection method	1343:1384	labeling	1356:1363	Collectively, our data reveals an effective labeling and detection method for gp120, serving as a tool for functional characterization of the gp120 glycans and potentially other glycosylated proteins.					
30115684	5	74	theme	glycopeptides	1026:1038	arg1	spectrometry					1002:1013	mass spectrometry	997:1013	mass spectrometry of tryptic glycopeptides	997:1038	Selective labeling of gp120 by N-azidoacetylmannosamine (ManNAz) and N-azidoacetylgalactosamine (GalNAz) incorporation into the gp120 glycan shield was characterized by mass spectrometry of tryptic glycopeptides.					
29617676	3	0	theme	N-linked	664:671	arg1	glycans					673:679	the complex N-linked glycans	652:679	the complex N-linked glycans	652:679	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	0	1	theme	Sialic-Acid-Binding	67:85	arg1	Viruses					87:93	Other Sialic-Acid-Binding Viruses	61:93	Other Sialic-Acid-Binding Viruses	61:93	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	5	2	theme	C-terminal	846:855	arg1	tail					857:860	the C-terminal tail	842:860	the C-terminal tail of IgA subtypes	842:876	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	1	3	theme	commensal	234:242	arg1	flora					254:258	the commensal bacterial flora	230:258	the commensal bacterial flora	230:258	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	2	4	theme	sialic	565:570	arg1	acid					572:575	sialic acid	565:575	sialic acid	565:575	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	2	4	theme	sialic	565:570	arg1	receptor					582:589	a receptor	580:589	a receptor	580:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	1	5	dep	plays	119:123	arg1	maintaining					203:213	maintaining	203:213	maintaining a balance with the commensal bacterial flora	203:258	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	1	5	dep	plays	119:123	arg1	extending					268:276	extending	268:276	extending maternal immunity via breast feeding	268:313	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	1	5	dep	plays	119:123	arg1	protecting					146:155	protecting	146:155	protecting our mucosal surfaces from viral infection	146:197	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	1	6	theme	bacterial	244:252	arg1	flora					254:258	the commensal bacterial flora	230:258	the commensal bacterial flora	230:258	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	2	7	theme	cell-surface	489:500	arg1	attachment					502:511	cell-surface attachment	489:511	cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	489:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	4	8	theme	binding	796:802	arg1	sites					804:808	the antigen binding sites	784:808	the antigen binding sites	784:808	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	1	9	theme	mucosal	161:167	arg1	surfaces					169:176	our mucosal surfaces	157:176	our mucosal surfaces	157:176	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	3	10	located	found	643:647	arg2	acid					638:641	sialic acid	631:641	sialic acid found in the complex N-linked glycans at position 459	631:695	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	3	10	located	found	643:647	arg1	position					684:691	position 459	684:695	position 459	684:695	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	3	10	located	found	643:647	arg1	glycans					673:679	the complex N-linked glycans	652:679	the complex N-linked glycans	652:679	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	2	11	theme	unique	449:454	arg1	tail					444:447	the C-terminal tail	429:447	the C-terminal tail unique to IgA molecules	429:471	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	4	12	theme	Antiviral	698:706	arg1	activity					708:715	Antiviral activity	698:715	Antiviral activity	698:715	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	2	13	theme	immune	353:358	arg1	function					369:376	an additional innate immune effector function	332:376	an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	332:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	5	14	theme	neuraminidases	980:993	arg1	defense					905:911	an innate line of defense	887:911	an innate line of defense against viruses that use sialic acid as a receptor	887:962	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	5	14	theme	neuraminidases	980:993	arg1	role					972:975	the role	968:975	the role of neuraminidases present on these virions	968:1018	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	1	15	theme	Immunoglobulin	96:109	arg1	IgA					114:116	IgA	114:116	IgA	114:116	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	1	15	theme	Immunoglobulin	96:109	arg1	A					111:111	Immunoglobulin A	96:111	Immunoglobulin A (IgA)	96:117	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	2	16	theme	innate	346:351	arg1	function					369:376	an additional innate immune effector function	332:376	an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	332:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	0	17	theme	IgA	23:25	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of Human IgA	0:25	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	5	18	theme	sialic	938:943	arg1	acid					945:948	sialic acid	938:948	sialic acid	938:948	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	5	18	theme	sialic	938:943	arg1	receptor					955:962	a receptor	953:962	a receptor	953:962	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	5	19	theme	innate	890:895	arg1	defense					905:911	an innate line of defense	887:911	an innate line of defense against viruses that use sialic acid as a receptor	887:962	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	2	20	theme	molecules	391:399	arg1	function					369:376	an additional innate immune effector function	332:376	an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	332:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	0	21	theme	Human	17:21	arg1	IgA					23:25	Human IgA	17:25	Human IgA	17:25	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	2	22	theme	A	526:526	arg1	attachment					502:511	cell-surface attachment	489:511	cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	489:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	5	23	theme	present	995:1001	arg1	neuraminidases					980:993	neuraminidases	980:993	neuraminidases present on these virions	980:1018	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	5	24	theme	line	897:900	arg1	defense					905:911	an innate line of defense	887:911	an innate line of defense against viruses that use sialic acid as a receptor	887:962	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	2	25	theme	IgA	387:389	arg1	molecules					391:399	human IgA molecules	381:399	human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	381:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	2	26	theme	additional	335:344	arg1	function					369:376	an additional innate immune effector function	332:376	an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	332:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	2	27	theme	influenza	516:524	arg1	A					526:526	influenza A	516:526	influenza A	516:526	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	5	28	attach	present	995:1001	arg2	neuraminidases					980:993	neuraminidases	980:993	neuraminidases present on these virions	980:1018	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	5	28	attach	present	995:1001	arg1	virions					1012:1018	these virions	1006:1018	these virions	1006:1018	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	0	29	gly	Glycosylation	0:12	arg1	IgA					23:25	Human IgA	17:25	Human IgA	17:25	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	3	30	link	N-linked	664:671	arg1	glycans					673:679	the complex N-linked glycans	652:679	the complex N-linked glycans	652:679	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	2	31	theme	human	381:385	arg1	molecules					391:399	human IgA molecules	381:399	human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	381:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	2	32	theme	C-terminal	433:442	arg1	tail					444:447	the C-terminal tail	429:447	the C-terminal tail unique to IgA molecules	429:471	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	1	33	theme	maternal	278:285	arg1	immunity					287:294	maternal immunity	278:294	maternal immunity	278:294	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	3	34	theme	antiviral	597:605	arg1	activity					607:614	This antiviral activity	592:614	This antiviral activity	592:614	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	4	35	dep	binding	772:778	arg1	absence					742:748	absence	742:748	absence	742:748	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	4	35	dep	binding	772:778	arg1	the					738:740	the	738:740	the	738:740	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	4	36	theme	antigen	788:794	arg1	sites					804:808	the antigen binding sites	784:808	the antigen binding sites	784:808	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	0	37	theme	Influenza	45:53	arg1	A					55:55	Influenza A	45:55	Influenza A	45:55	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	2	38	theme	viruses	548:554	arg1	attachment					502:511	cell-surface attachment	489:511	cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	489:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	1	39	theme	breast	300:305	arg1	feeding					307:313	breast feeding	300:313	breast feeding	300:313	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	2	40	theme	enveloped	538:546	arg1	viruses					548:554	other enveloped viruses	532:554	other enveloped viruses that use sialic acid as a receptor	532:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	5	41	theme	IgA	865:867	arg1	subtypes					869:876	IgA subtypes	865:876	IgA subtypes	865:876	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	2	42	theme	other	532:536	arg1	viruses					548:554	other enveloped viruses	532:554	other enveloped viruses that use sialic acid as a receptor	532:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	5	43	theme	of	902:903	arg1	defense					905:911	an innate line of defense	887:911	an innate line of defense against viruses that use sialic acid as a receptor	887:962	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	1	44	with	balance	217:223	arg1	flora					254:258	the commensal bacterial flora	230:258	the commensal bacterial flora	230:258	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	5	45	theme	subtypes	869:876	arg1	tail					857:860	the C-terminal tail	842:860	the C-terminal tail of IgA subtypes	842:876	Our data, therefore, show that the C-terminal tail of IgA subtypes provides an innate line of defense against viruses that use sialic acid as a receptor and the role of neuraminidases present on these virions.					
29617676	0	46	theme	Other	61:65	arg1	Viruses					87:93	Other Sialic-Acid-Binding Viruses	61:93	Other Sialic-Acid-Binding Viruses	61:93	Glycosylation of Human IgA Directly Inhibits Influenza A and Other Sialic-Acid-Binding Viruses.					
29617676	3	47	theme	sialic	631:636	arg1	acid					638:641	sialic acid	631:641	sialic acid found in the complex N-linked glycans at position 459	631:695	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	4	48	theme	antibody	763:770	arg1	binding					772:778	classical antibody binding	753:778	classical antibody binding	753:778	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29617676	1	49	theme	viral	183:187	arg1	infection					189:197	viral infection	183:197	viral infection	183:197	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	1	50	theme	important	128:136	arg1	role					138:141	an important role	125:141	an important role	125:141	Immunoglobulin A (IgA) plays an important role in protecting our mucosal surfaces from viral infection, in maintaining a balance with the commensal bacterial flora, and in extending maternal immunity via breast feeding.					
29617676	2	51	theme	effector	360:367	arg1	function					369:376	an additional innate immune effector function	332:376	an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor	332:589	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	2	52	theme	IgA	459:461	arg1	molecules					463:471	IgA molecules	459:471	IgA molecules	459:471	Here, we report an additional innate immune effector function of human IgA molecules in that we demonstrate that the C-terminal tail unique to IgA molecules interferes with cell-surface attachment of influenza A and other enveloped viruses that use sialic acid as a receptor.					
29617676	3	53	theme	complex	656:662	arg1	glycans					673:679	the complex N-linked glycans	652:679	the complex N-linked glycans	652:679	This antiviral activity is mediated by sialic acid found in the complex N-linked glycans at position 459.					
29617676	4	54	theme	classical	753:761	arg1	binding					772:778	classical antibody binding	753:778	classical antibody binding	753:778	Antiviral activity was observed even in the absence of classical antibody binding via the antigen binding sites.					
29346724	10	0	theme	secondary	1344:1352	arg1	structure					1354:1362	the secondary structure	1340:1362	the secondary structure	1340:1362	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	8	1	theme	proper	1072:1077	arg1	folding					1087:1093	proper protein folding	1072:1093	proper protein folding	1072:1093	Our data show that EXTL3ΔN has N-glycans at least at two positions, Asn290 and Asn592, which seem to be critical for proper protein folding and/or release.					
29346724	2	2	theme	cellular	353:360	arg1	communication					362:374	cellular communication	353:374	cellular communication	353:374	HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling.					
29346724	12	3	theme	bioinformatics	1595:1608	arg1	analyses					1610:1617	bioinformatics analyses	1595:1617	bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain	1595:1711	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	11	4	theme	broad	1550:1554	arg1	regions					1530:1536	two distinct regions	1517:1536	two distinct regions	1517:1536	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	11	5	theme	light	1415:1419	arg1	scattering					1421:1430	dynamic light scattering	1407:1430	dynamic light scattering	1407:1430	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	4	6	theme	EXTL3	506:510	arg1	enzyme					512:517	The EXTL3 enzyme	502:517	The EXTL3 enzyme	502:517	The EXTL3 enzyme mainly works as an initiator in HS biosynthesis.					
29346724	5	7	theme	human	582:586	arg1	EXTL3ΔN					618:624	EXTL3ΔN	618:624	EXTL3ΔN	618:624	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	5	7	theme	human	582:586	arg1	EXTL3					611:615	human lumenal N-glycosylated EXTL3	582:615	human lumenal N-glycosylated EXTL3 (EXTL3ΔN)	582:625	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	1	8	theme	cell	252:255	arg1	surfaces					257:264	cell surfaces	252:264	cell surfaces	252:264	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	10	9	theme	sheets	1281:1286	arg1	fraction					1267:1274	a substantial fraction	1253:1274	a substantial fraction of β sheets (25%)	1253:1292	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	5	10	theme	lumenal	588:594	arg1	EXTL3ΔN					618:624	EXTL3ΔN	618:624	EXTL3ΔN	618:624	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	5	10	theme	lumenal	588:594	arg1	EXTL3					611:615	human lumenal N-glycosylated EXTL3	582:615	human lumenal N-glycosylated EXTL3 (EXTL3ΔN)	582:625	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	7	11	from	stability	864:872	arg1	solution					926:933	solution	926:933	solution	926:933	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	10	12	theme	spectrum	1235:1242	arg1	Deconvolution					1196:1208	Deconvolution	1196:1208	Deconvolution of the EXTL3ΔN far-UV CD spectrum	1196:1242	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	10	13	theme	EXTL3ΔN	1217:1223	arg1	spectrum					1235:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	5	14	theme	N-glycosylated	596:609	arg1	EXTL3ΔN					618:624	EXTL3ΔN	618:624	EXTL3ΔN	618:624	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	5	14	theme	N-glycosylated	596:609	arg1	EXTL3					611:615	human lumenal N-glycosylated EXTL3	582:615	human lumenal N-glycosylated EXTL3 (EXTL3ΔN)	582:625	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	2	15	theme	HS	267:268	arg1	chains					270:275	HS chains	267:275	HS chains	267:275	HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling.					
29346724	10	16	theme	CD	1232:1233	arg1	spectrum					1235:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	0	17	theme	Solution	100:107	arg1	Structure					109:117	Solution Structure	100:117	Solution Structure	100:117	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	11	18	theme	arrangement	1487:1497	arg1	suggestive					1463:1472	suggestive	1463:1472	suggestive	1463:1472	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	0	19	theme	Structural	0:9	arg1	Characterization					27:42	Structural and Biophysical Characterization	0:42	Structural and Biophysical Characterization of Human EXTL3	0:57	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	10	20	theme	far-UV	1225:1230	arg1	spectrum					1235:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	the EXTL3ΔN far-UV CD spectrum	1213:1242	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	4	21	theme	HS	551:552	arg1	biosynthesis					554:565	HS biosynthesis	551:565	HS biosynthesis	551:565	The EXTL3 enzyme mainly works as an initiator in HS biosynthesis.					
29346724	7	22	dep	stability	864:872	arg1	the					860:862	the	860:862	the	860:862	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	2	23	theme	many	303:306	arg1	molecules					331:339	many biologically important molecules	303:339	many biologically important molecules involved in cellular communication and signaling	303:388	HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling.					
29346724	5	24	theme	human	652:656	arg1	cells					675:679	human embryonic kidney cells	652:679	human embryonic kidney cells	652:679	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	3	25	theme	exostosin	395:403	arg1	glycosyltransferases					424:443	glycosyltransferases	424:443	glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs	424:499	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	3	25	theme	exostosin	395:403	arg1	proteins					411:418	The exostosin (EXT) proteins	391:418	The exostosin (EXT) proteins	391:418	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	12	26	with	consistent	1579:1588	arg1	analyses					1610:1617	bioinformatics analyses	1595:1617	bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain	1595:1711	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	7	27	theme	protein	915:921	arg1	conformation					878:889	conformation	878:889	conformation	878:889	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	7	27	theme	protein	915:921	arg1	stability					864:872	stability	864:872	stability	864:872	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	7	28	from	conformation	878:889	arg1	solution					926:933	solution	926:933	solution	926:933	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	0	29	theme	EXTL3	53:57	arg1	Characterization					27:42	Structural and Biophysical Characterization	0:42	Structural and Biophysical Characterization of Human EXTL3	0:57	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	1	30	theme	heparan	192:198	arg1	HS					209:210	HS	209:210	HS	209:210	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	30	theme	heparan	192:198	arg1	sulfate					200:206	one or more heparan sulfate	180:206	one or more heparan sulfate (HS) polysaccharides	180:227	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	12	31	theme	coiled-coil	1694:1704	arg1	domain					1706:1711	a coiled-coil domain	1692:1711	a coiled-coil domain	1692:1711	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	6	32	theme	N-glycosylation	779:793	arg1	sites					795:799	the N-glycosylation sites	775:799	the N-glycosylation sites	775:799	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	6	32	theme	N-glycosylation	779:793	arg1	N-glycans					836:844	their attached N-glycans	821:844	their attached N-glycans	821:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	0	33	dep	Organization	67:78	arg1	Characterization					27:42	Structural and Biophysical Characterization	0:42	Structural and Biophysical Characterization of Human EXTL3	0:57	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	10	34	theme	substantial	1255:1265	arg1	fraction					1267:1274	a substantial fraction	1253:1274	a substantial fraction of β sheets (25%)	1253:1292	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	1	35	theme	sulfate	200:206	arg1	polysaccharides					213:227	one or more heparan sulfate (HS) polysaccharides	180:227	one or more heparan sulfate (HS) polysaccharides	180:227	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	0	36	theme	Biophysical	15:25	arg1	Characterization					27:42	Structural and Biophysical Characterization	0:42	Structural and Biophysical Characterization of Human EXTL3	0:57	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	12	37	with	structure	1641:1649	arg1	domain					1706:1711	a coiled-coil domain	1692:1711	a coiled-coil domain	1692:1711	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	12	37	with	structure	1641:1649	arg1	domains					1680:1686	two glycosyltransferase domains	1656:1686	two glycosyltransferase domains	1656:1686	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	11	38	theme	Solution	1365:1372	arg1	scattering					1392:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	0	39	theme	Human	47:51	arg1	EXTL3					53:57	Human EXTL3	47:57	Human EXTL3	47:57	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	2	40	theme	molecules	331:339	arg1	activity					291:298	the activity	287:298	the activity of many biologically important molecules involved in cellular communication and signaling	287:388	HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling.					
29346724	3	41	theme	Golgi	452:456	arg1	apparatus					458:466	the Golgi apparatus	448:466	the Golgi apparatus	448:466	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	8	42	contain	has	982:984	arg2	N-glycans					986:994	N-glycans	986:994	N-glycans	986:994	Our data show that EXTL3ΔN has N-glycans at least at two positions, Asn290 and Asn592, which seem to be critical for proper protein folding and/or release.					
29346724	8	42	contain	has	982:984	arg1	EXTL3ΔN					974:980	EXTL3ΔN	974:980	EXTL3ΔN	974:980	Our data show that EXTL3ΔN has N-glycans at least at two positions, Asn290 and Asn592, which seem to be critical for proper protein folding and/or release.					
29346724	6	43	theme	biophysical	704:714	arg1	approaches					732:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	9	44	theme	high	1139:1142	arg1	temperature					1144:1154	high temperature	1139:1154	high temperature (∼59 °C)	1139:1163	EXTL3ΔN is quite stable, as high temperature (∼59 °C) was required for denaturation.					
29346724	9	44	theme	high	1139:1142	arg1	°C					1161:1162	∼59 °C	1157:1162	∼59 °C	1157:1162	EXTL3ΔN is quite stable, as high temperature (∼59 °C) was required for denaturation.					
29346724	6	45	theme	N-glycans	836:844	arg1	sites					795:799	the N-glycosylation sites	775:799	the N-glycosylation sites	775:799	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	6	45	theme	N-glycans	836:844	arg1	function					809:816	the function	805:816	the function of their attached N-glycans	805:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	6	45	theme	N-glycans	836:844	arg1	N-glycans					836:844	their attached N-glycans	821:844	their attached N-glycans	821:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	8	46	theme	protein	1079:1085	arg1	folding					1087:1093	proper protein folding	1072:1093	proper protein folding	1072:1093	Our data show that EXTL3ΔN has N-glycans at least at two positions, Asn290 and Asn592, which seem to be critical for proper protein folding and/or release.					
29346724	3	47	theme	EXT	406:408	arg1	glycosyltransferases					424:443	glycosyltransferases	424:443	glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs	424:499	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	3	47	theme	EXT	406:408	arg1	proteins					411:418	The exostosin (EXT) proteins	391:418	The exostosin (EXT) proteins	391:418	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	6	48	gly	N-glycosylation	779:793	arg2	sites					795:799	the N-glycosylation sites	775:799	the N-glycosylation sites	775:799	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	6	48	gly	N-glycosylation	779:793	arg2	N-glycans					836:844	their attached N-glycans	821:844	their attached N-glycans	821:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	6	48	gly	N-glycosylation	779:793	arg1	N-glycans					836:844	their attached N-glycans	821:844	their attached N-glycans	821:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	3	49	theme	HS	482:483	arg1	chains					485:490	HS chains	482:490	HS chains	482:490	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	6	50	theme	attached	827:834	arg1	N-glycans					836:844	their attached N-glycans	821:844	their attached N-glycans	821:844	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	1	51	located	found	230:234	arg2	proteins					154:161	proteins	154:161	proteins	154:161	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	51	located	found	230:234	arg2	proteoglycans					136:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	51	located	found	230:234	arg1	abundance					239:247	abundance	239:247	abundance	239:247	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	51	located	found	230:234	arg1	surfaces					257:264	cell surfaces	252:264	cell surfaces	252:264	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	10	52	theme	β	1279:1279	arg1	sheets					1281:1286	β sheets	1279:1286	β sheets (25%)	1279:1292	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	10	52	theme	β	1279:1279	arg1	%					1291:1291	25%	1289:1291	25%	1289:1291	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	1	53	theme	Heparan	120:126	arg1	proteins					154:161	proteins	154:161	proteins	154:161	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	53	theme	Heparan	120:126	arg1	proteoglycans					136:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	7	54	theme	purified	898:905	arg1	protein					915:921	the purified EXTL3ΔN protein	894:921	the purified EXTL3ΔN protein	894:921	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	10	55	theme	α-helices	1321:1329	arg1	proportion					1307:1316	a minor proportion	1299:1316	a minor proportion of α-helices (14%)	1299:1335	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	5	56	theme	embryonic	658:666	arg1	cells					675:679	human embryonic kidney cells	652:679	human embryonic kidney cells	652:679	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	11	57	theme	extended	1444:1451	arg1	structure					1453:1461	an extended structure	1441:1461	an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively	1441:1568	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	6	58	theme	biochemical	720:730	arg1	approaches					732:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	1	59	theme	sulfate	128:134	arg1	proteins					154:161	proteins	154:161	proteins	154:161	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	1	59	theme	sulfate	128:134	arg1	proteoglycans					136:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans	120:148	Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces.					
29346724	7	60	theme	EXTL3ΔN	907:913	arg1	protein					915:921	the purified EXTL3ΔN protein	894:921	the purified EXTL3ΔN protein	894:921	Furthermore, the stability and conformation of the purified EXTL3ΔN protein in solution have been analyzed.					
29346724	5	61	theme	kidney	668:673	arg1	cells					675:679	human embryonic kidney cells	652:679	human embryonic kidney cells	652:679	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	11	62	theme	narrow	1539:1544	arg1	regions					1530:1536	two distinct regions	1517:1536	two distinct regions	1517:1536	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	11	63	theme	small-angle	1374:1384	arg1	scattering					1392:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	11	64	theme	X-ray	1386:1390	arg1	scattering					1392:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering	1365:1401	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	2	65	theme	important	321:329	arg1	molecules					331:339	many biologically important molecules	303:339	many biologically important molecules involved in cellular communication and signaling	303:388	HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling.					
29346724	11	66	dep	structure	1453:1461	arg1	suggestive					1463:1472	suggestive	1463:1472	suggestive	1463:1472	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	11	66	dep	structure	1453:1461	arg1	consisting					1503:1512	consisting	1503:1512	consisting	1503:1512	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	11	67	theme	distinct	1521:1528	arg1	regions					1530:1536	two distinct regions	1517:1536	two distinct regions	1517:1536	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	3	68	from	glycosyltransferases	424:443	arg1	apparatus					458:466	the Golgi apparatus	448:466	the Golgi apparatus	448:466	The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs.					
29346724	10	69	theme	minor	1301:1305	arg1	proportion					1307:1316	a minor proportion	1299:1316	a minor proportion of α-helices (14%)	1299:1335	Deconvolution of the EXTL3ΔN far-UV CD spectrum revealed a substantial fraction of β sheets (25%) with a minor proportion of α-helices (14%) in the secondary structure.					
29346724	11	70	theme	dynamic	1407:1413	arg1	scattering					1421:1430	dynamic light scattering	1407:1430	dynamic light scattering	1407:1430	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	5	71	gly	N-glycosylated	596:609	arg1	EXTL3ΔN					618:624	EXTL3ΔN	618:624	EXTL3ΔN	618:624	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	5	71	gly	N-glycosylated	596:609	arg1	EXTL3					611:615	human lumenal N-glycosylated EXTL3	582:615	human lumenal N-glycosylated EXTL3 (EXTL3ΔN)	582:625	In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells, and purified.					
29346724	0	72	theme	Domain	60:65	arg1	Organization					67:78	Domain Organization	60:78	Domain Organization	60:78	Structural and Biophysical Characterization of Human EXTL3: Domain Organization, Glycosylation, and Solution Structure.					
29346724	11	73	theme	dimeric	1479:1485	arg1	arrangement					1487:1497	a dimeric arrangement	1477:1497	a dimeric arrangement	1477:1497	Solution small-angle X-ray scattering and dynamic light scattering revealed an extended structure suggestive of a dimeric arrangement and consisting of two distinct regions, narrow and broad, respectively.					
29346724	4	74	from	initiator	538:546	arg1	biosynthesis					554:565	HS biosynthesis	551:565	HS biosynthesis	551:565	The EXTL3 enzyme mainly works as an initiator in HS biosynthesis.					
29346724	6	75	theme	Various	696:702	arg1	approaches					732:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches	696:741	Various biophysical and biochemical approaches were then employed to elucidate the N-glycosylation sites and the function of their attached N-glycans.					
29346724	12	76	theme	glycosyltransferase	1660:1678	arg1	domains					1680:1686	two glycosyltransferase domains	1656:1686	two glycosyltransferase domains	1656:1686	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29346724	12	77	theme	3-domain	1632:1639	arg1	structure					1641:1649	a 3-domain structure	1630:1649	a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain	1630:1711	This is consistent with bioinformatics analyses suggesting a 3-domain structure with two glycosyltransferase domains and a coiled-coil domain.					
29888865	6	0	theme	physical	920:927	arg1	properties					955:964	its physical, functional and antigenic properties	916:964	its physical, functional and antigenic properties	916:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	13	1	theme	distinct	2197:2204	arg1	LacdiNAc					2281:2288	fucosylated LacdiNAc	2269:2288	fucosylated LacdiNAc (FucLDN)	2269:2297	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	1	theme	distinct	2197:2204	arg1	LewisX/Y					2307:2314	LewisX/Y	2307:2314	LewisX/Y	2307:2314	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	1	theme	distinct	2197:2204	arg1	epitopes					2235:2242	distinct and immunologically relevant epitopes	2197:2242	distinct and immunologically relevant epitopes	2197:2242	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	1	theme	distinct	2197:2204	arg1	NeuGc					2300:2304	NeuGc	2300:2304	NeuGc	2300:2304	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	1	theme	distinct	2197:2204	arg1	epitopes					2332:2339	Galα1,3 Gal epitopes	2320:2339	Galα1,3 Gal epitopes	2320:2339	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	1	theme	distinct	2197:2204	arg1	LacdiNAc					2253:2260	LacdiNAc	2253:2260	LacdiNAc (LDN)	2253:2266	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	5	2	theme	plasma	583:588	arg1	glycoprotein					590:601	a plasma glycoprotein	581:601	a plasma glycoprotein	581:601	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	2	theme	plasma	583:588	arg1	VIII					565:568	Background/Objective Human factor VIII	531:568	Background/Objective Human factor VIII (FVIII)	531:576	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	2	theme	plasma	583:588	arg1	SUMMARY					522:528	SUMMARY	522:528	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.	522:810	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	2	theme	plasma	583:588	arg1	defects					604:610	defects	604:610	defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion	604:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	4	3	theme	relevant	503:510	arg1	epitopes					512:519	distinct and immunologically relevant epitopes	474:519	distinct and immunologically relevant epitopes	474:519	Recombinant FVIIIs expressed distinct and immunologically relevant epitopes.					
29888865	11	4	theme	acid	1797:1800	arg1	evidence					1764:1771	evidence	1764:1771	evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII	1764:1825	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	6	5	theme	substantial	891:901	arg1	influence					903:911	a substantial influence	889:911	a substantial influence on its physical, functional and antigenic properties	889:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	1	6	theme	proteins	206:213	arg1	heterogeneity					177:189	Essentials Glycosylation heterogeneity	152:189	Essentials Glycosylation heterogeneity of recombinant proteins	152:213	Essentials Glycosylation heterogeneity of recombinant proteins affects pharmacokinetics and immunogenicity.					
29888865	4	7	theme	Recombinant	445:455	arg1	FVIIIs					457:462	Recombinant FVIIIs	445:462	Recombinant FVIIIs	445:462	Recombinant FVIIIs expressed distinct and immunologically relevant epitopes.					
29888865	5	8	theme	cell	737:740	arg1	lines					742:746	various cell lines	729:746	various cell lines (recombinant FVIII)	729:766	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	8	theme	cell	737:740	arg1	FVIII					761:765	recombinant FVIII	749:765	recombinant FVIII	749:765	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	11	9	theme	pdFVIII	1697:1703	arg1	repertoire					1683:1692	the N-glycan repertoire	1670:1692	the N-glycan repertoire of pdFVIII to an unprecedented level	1670:1729	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	9	theme	pdFVIII	1697:1703	arg1	pdFVIII					1697:1703	pdFVIII	1697:1703	pdFVIII	1697:1703	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	5	10	theme	FVIII	679:683	arg1	products					685:692	FVIII products	679:692	FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion	679:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	11	theme	B-domain	793:800	arg1	deletion					802:809	B-domain deletion	793:809	B-domain deletion	793:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	13	12	theme	fucosylated	2269:2279	arg1	LacdiNAc					2281:2288	fucosylated LacdiNAc	2269:2288	fucosylated LacdiNAc (FucLDN)	2269:2297	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	12	theme	fucosylated	2269:2279	arg1	FucLDN					2291:2296	FucLDN	2291:2296	FucLDN	2291:2296	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	3	13	link	plasma-derived	409:422	arg1	FVIII					424:428	plasma-derived FVIII	409:428	plasma-derived FVIII	409:428	Depending on cell line, significant differences to plasma-derived FVIII were observed.					
29888865	2	14	theme	Factor	306:311	arg1	VIII					313:316	plasma-derived Factor VIII	291:316	plasma-derived Factor VIII	291:316	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	9	15	theme	rFVIII	1376:1381	arg1	products					1383:1390	six rFVIII products	1372:1390	six rFVIII products expressed in CHO, BHK or HEK cell lines	1372:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	5	16	theme	Current	645:651	arg1	therapy					666:672	A. Current substitution therapy	642:672	A. Current substitution therapy	642:672	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	12	17	theme	site-specific	1837:1849	arg1	glycosylation					1851:1863	site-specific glycosylation	1837:1863	site-specific glycosylation of rFVIII	1837:1873	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	13	18	theme	common	2079:2084	arg1	epitopes					2070:2077	biologically important epitopes	2047:2077	biologically important epitopes common to all samples (i.e. sialylation and high-mannose)	2047:2135	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	2	19	link	plasma-derived	291:304	arg1	VIII					313:316	plasma-derived Factor VIII	291:316	plasma-derived Factor VIII	291:316	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	1	20	gly	heterogeneity	177:189	arg1	proteins					206:213	recombinant proteins	194:213	recombinant proteins	194:213	Essentials Glycosylation heterogeneity of recombinant proteins affects pharmacokinetics and immunogenicity.					
29888865	2	21	theme	recombinant	324:334	arg1	FVIIIs					336:341	6 recombinant FVIIIs	322:341	6 recombinant FVIIIs	322:341	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	0	22	theme	VIII	70:73	arg1	N-glycosylation					23:37	N-glycosylation	23:37	N-glycosylation of human plasma-derived factor VIII and different recombinant products	23:108	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	9	23	theme	CHO	1405:1407	arg1	lines					1426:1430	CHO, BHK or HEK cell lines	1405:1430	lines	1426:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	1	24	theme	Glycosylation	163:175	arg1	heterogeneity					177:189	Essentials Glycosylation heterogeneity	152:189	Essentials Glycosylation heterogeneity of recombinant proteins	152:213	Essentials Glycosylation heterogeneity of recombinant proteins affects pharmacokinetics and immunogenicity.					
29888865	3	25	theme	significant	382:392	arg1	differences					394:404	significant differences	382:404	significant differences to plasma-derived FVIII	382:428	Depending on cell line, significant differences to plasma-derived FVIII were observed.					
29888865	0	26	theme	different	79:87	arg1	products					101:108	different recombinant products	79:108	different recombinant products	79:108	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	9	27	theme	BHK	1410:1412	arg1	lines					1426:1430	CHO, BHK or HEK cell lines	1405:1430	lines	1426:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	8	28	theme	FVIII	1139:1143	arg1	glycosylation					1145:1157	FVIII glycosylation	1139:1157	FVIII glycosylation	1139:1157	However, the literature on FVIII glycosylation is inconsistent, preventing assembly into a coherent model.					
29888865	12	29	gly	glycosylation	1851:1863	arg1	rFVIII					1868:1873	rFVIII	1868:1873	rFVIII	1868:1873	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	13	30	theme	important	2060:2068	arg1	epitopes					2070:2077	biologically important epitopes	2047:2077	biologically important epitopes common to all samples (i.e. sialylation and high-mannose)	2047:2135	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	0	31	theme	products	101:108	arg1	N-glycosylation					23:37	N-glycosylation	23:37	N-glycosylation of human plasma-derived factor VIII and different recombinant products	23:108	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	9	32	theme	HEK	1417:1419	arg1	lines					1426:1430	CHO, BHK or HEK cell lines	1405:1430	lines	1426:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	5	33	theme	human	708:712	arg1	plasma					714:719	human plasma	708:719	human plasma	708:719	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	13	34	theme	relevant	2226:2233	arg1	LacdiNAc					2281:2288	fucosylated LacdiNAc	2269:2288	fucosylated LacdiNAc (FucLDN)	2269:2297	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	34	theme	relevant	2226:2233	arg1	LewisX/Y					2307:2314	LewisX/Y	2307:2314	LewisX/Y	2307:2314	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	34	theme	relevant	2226:2233	arg1	epitopes					2235:2242	distinct and immunologically relevant epitopes	2197:2242	distinct and immunologically relevant epitopes	2197:2242	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	34	theme	relevant	2226:2233	arg1	NeuGc					2300:2304	NeuGc	2300:2304	NeuGc	2300:2304	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	34	theme	relevant	2226:2233	arg1	epitopes					2332:2339	Galα1,3 Gal epitopes	2320:2339	Galα1,3 Gal epitopes	2320:2339	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	34	theme	relevant	2226:2233	arg1	LacdiNAc					2253:2260	LacdiNAc	2253:2260	LacdiNAc (LDN)	2253:2266	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	6	35	from	influence	903:911	arg1	properties					955:964	its physical, functional and antigenic properties	916:964	its physical, functional and antigenic properties	916:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	0	36	theme	In-depth	0:7	arg1	comparison					9:18	In-depth comparison	0:18	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.	0:150	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	7	37	theme	FVIII	1026:1030	arg1	products					1032:1039	FVIII products	1026:1039	FVIII products	1026:1039	Variation in glycosylation is likely to be the reason that FVIII products differ in their pharmacokinetics, pharmacodynamics and immunogenicity.					
29888865	11	38	theme	unprecedented	1711:1723	arg1	level					1725:1729	an unprecedented level	1708:1729	an unprecedented level	1708:1729	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	14	39	theme	glycomic	2392:2399	arg1	differences					2401:2411	the greatest glycomic differences	2379:2411	the greatest glycomic differences	2379:2411	rFVIII expressed in HEK cells showed the greatest glycomic differences to human pdFVIII.					
29888865	11	40	located	found	1810:1814	arg1	pdFVIII					1819:1825	pdFVIII	1819:1825	pdFVIII	1819:1825	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	40	located	found	1810:1814	arg2	NeuGc					1803:1807	NeuGc	1803:1807	NeuGc	1803:1807	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	40	located	found	1810:1814	arg2	acid					1797:1800	N-glycolylneuraminic acid	1776:1800	N-glycolylneuraminic acid (NeuGc) found on pdFVIII	1776:1825	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	12	41	theme	N-glycan	1954:1961	arg1	content					1963:1969	the entire N-glycan content	1943:1969	the entire N-glycan content of each sample	1943:1984	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	9	42	theme	glycosylation	1252:1264	arg1	mechanisms					1266:1275	the glycosylation mechanisms	1248:1275	the glycosylation mechanisms underlying FVIII biology	1248:1300	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	0	43	theme	plasma-derived	48:61	arg1	VIII					70:73	human plasma-derived factor VIII	42:73	human plasma-derived factor VIII	42:73	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	12	44	theme	rFVIII	1868:1873	arg1	glycosylation					1851:1863	site-specific glycosylation	1837:1863	site-specific glycosylation of rFVIII	1837:1873	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	6	45	theme	antigenic	945:953	arg1	properties					955:964	its physical, functional and antigenic properties	916:964	its physical, functional and antigenic properties	916:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	0	46	gly	N-glycosylation	23:37	arg1	VIII					70:73	human plasma-derived factor VIII	42:73	human plasma-derived factor VIII	42:73	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	0	46	gly	N-glycosylation	23:37	arg1	products					101:108	different recombinant products	79:108	different recombinant products	79:108	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	6	47	theme	protein	850:856	arg1	modification					858:869	a post-translational protein modification	829:869	a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties	829:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	6	47	theme	protein	850:856	arg1	Glycosylation					812:824	Glycosylation	812:824	Glycosylation	812:824	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	5	48	theme	factor	558:563	arg1	glycoprotein					590:601	a plasma glycoprotein	581:601	a plasma glycoprotein	581:601	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	48	theme	factor	558:563	arg1	FVIII					571:575	FVIII	571:575	FVIII	571:575	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	48	theme	factor	558:563	arg1	VIII					565:568	Background/Objective Human factor VIII	531:568	Background/Objective Human factor VIII (FVIII)	531:576	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	48	theme	factor	558:563	arg1	SUMMARY					522:528	SUMMARY	522:528	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.	522:810	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	6	49	theme	functional	930:939	arg1	properties					955:964	its physical, functional and antigenic properties	916:964	its physical, functional and antigenic properties	916:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	12	50	with	consistent	1882:1891	arg1	pdFVIII					1898:1904	pdFVIII	1898:1904	pdFVIII	1898:1904	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	7	51	from	Variation	967:975	arg1	glycosylation					980:992	glycosylation	980:992	glycosylation	980:992	Variation in glycosylation is likely to be the reason that FVIII products differ in their pharmacokinetics, pharmacodynamics and immunogenicity.					
29888865	10	52	theme	head-to-head	1473:1484	arg1	glycomic					1495:1502	head-to-head detailed glycomic	1473:1502	head-to-head detailed glycomic	1473:1502	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	13	53	theme	Galα1,3	2320:2326	arg1	epitopes					2332:2339	Galα1,3 Gal epitopes	2320:2339	Galα1,3 Gal epitopes	2320:2339	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	8	54	from	literature	1125:1134	arg1	glycosylation					1145:1157	FVIII glycosylation	1139:1157	FVIII glycosylation	1139:1157	However, the literature on FVIII glycosylation is inconsistent, preventing assembly into a coherent model.					
29888865	9	55	link	plasma-derived	1343:1356	arg1	FVIII					1362:1366	human plasma-derived (pd)FVIII	1337:1366	human plasma-derived (pd)FVIII	1337:1366	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	14	56	theme	HEK	2362:2364	arg1	cells					2366:2370	HEK cells	2362:2370	HEK cells	2362:2370	rFVIII expressed in HEK cells showed the greatest glycomic differences to human pdFVIII.					
29888865	11	57	theme	N-glycolylneuraminic	1776:1795	arg1	NeuGc					1803:1807	NeuGc	1803:1807	NeuGc	1803:1807	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	57	theme	N-glycolylneuraminic	1776:1795	arg1	acid					1797:1800	N-glycolylneuraminic acid	1776:1800	N-glycolylneuraminic acid (NeuGc) found on pdFVIII	1776:1825	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	58	theme	first	1744:1748	arg1	time					1750:1753	the first time	1740:1753	the first time	1740:1753	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	0	59	link	plasma-derived	48:61	arg1	VIII					70:73	human plasma-derived factor VIII	42:73	human plasma-derived factor VIII	42:73	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	5	60	theme	recombinant	749:759	arg1	lines					742:746	various cell lines	729:746	various cell lines (recombinant FVIII)	729:766	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	60	theme	recombinant	749:759	arg1	FVIII					761:765	recombinant FVIII	749:765	recombinant FVIII	749:765	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	10	61	theme	glycoproteomic	1508:1521	arg1	characterization					1523:1538	glycoproteomic characterization	1508:1538	glycoproteomic characterization	1508:1538	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	5	62	theme	Background/Objective	531:550	arg1	glycoprotein					590:601	a plasma glycoprotein	581:601	a plasma glycoprotein	581:601	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	62	theme	Background/Objective	531:550	arg1	FVIII					571:575	FVIII	571:575	FVIII	571:575	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	62	theme	Background/Objective	531:550	arg1	VIII					565:568	Background/Objective Human factor VIII	531:568	Background/Objective Human factor VIII (FVIII)	531:576	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	62	theme	Background/Objective	531:550	arg1	SUMMARY					522:528	SUMMARY	522:528	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.	522:810	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	11	63	theme	N-glycan	1674:1681	arg1	repertoire					1683:1692	the N-glycan repertoire	1670:1692	the N-glycan repertoire of pdFVIII to an unprecedented level	1670:1729	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	11	63	theme	N-glycan	1674:1681	arg1	pdFVIII					1697:1703	pdFVIII	1697:1703	pdFVIII	1697:1703	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	1	64	theme	recombinant	194:204	arg1	proteins					206:213	recombinant proteins	194:213	recombinant proteins	194:213	Essentials Glycosylation heterogeneity of recombinant proteins affects pharmacokinetics and immunogenicity.					
29888865	13	65	gly	fucosylated	2269:2279	arg1	LacdiNAc					2281:2288	fucosylated LacdiNAc	2269:2288	fucosylated LacdiNAc (FucLDN)	2269:2297	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	13	65	gly	fucosylated	2269:2279	arg1	FucLDN					2291:2296	FucLDN	2291:2296	FucLDN	2291:2296	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	5	66	theme	various	729:735	arg1	lines					742:746	various cell lines	729:746	various cell lines (recombinant FVIII)	729:766	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	66	theme	various	729:735	arg1	FVIII					761:765	recombinant FVIII	749:765	recombinant FVIII	749:765	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	9	67	theme	plasma-derived	1343:1356	arg1	FVIII					1362:1366	human plasma-derived (pd)FVIII	1337:1366	human plasma-derived (pd)FVIII	1337:1366	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	4	68	theme	distinct	474:481	arg1	epitopes					512:519	distinct and immunologically relevant epitopes	474:519	distinct and immunologically relevant epitopes	474:519	Recombinant FVIIIs expressed distinct and immunologically relevant epitopes.					
29888865	0	69	theme	clinical	129:136	arg1	implications					138:149	clinical implications	129:149	clinical implications	129:149	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	9	70	gly	N-glycosylation	1318:1332	arg1	products					1383:1390	six rFVIII products	1372:1390	six rFVIII products expressed in CHO, BHK or HEK cell lines	1372:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	9	70	gly	N-glycosylation	1318:1332	arg1	FVIII					1362:1366	human plasma-derived (pd)FVIII	1337:1366	human plasma-derived (pd)FVIII	1337:1366	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	5	71	theme	deletion	802:809	arg1	levels					783:788	different levels	773:788	different levels of B-domain deletion	773:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	9	72	theme	FVIII	1362:1366	arg1	N-glycosylation					1318:1332	the N-glycosylation	1314:1332	the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines	1314:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	2	73	theme	VIII	313:316	arg1	FVIIIs					336:341	6 recombinant FVIIIs	322:341	6 recombinant FVIIIs	322:341	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	2	73	theme	VIII	313:316	arg1	N-glycomics/glycoproteomics					260:286	N-glycomics/glycoproteomics	260:286	N-glycomics/glycoproteomics of plasma-derived Factor VIII	260:316	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	2	74	theme	plasma-derived	291:304	arg1	VIII					313:316	plasma-derived Factor VIII	291:316	plasma-derived Factor VIII	291:316	N-glycomics/glycoproteomics of plasma-derived Factor VIII and 6 recombinant FVIIIs were compared.					
29888865	5	75	theme	A.	642:643	arg1	therapy					666:672	A. Current substitution therapy	642:672	A. Current substitution therapy	642:672	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	0	76	from	structure	116:124	arg1	comparison					9:18	In-depth comparison	0:18	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.	0:150	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	13	77	theme	epitopes	2070:2077	arg1	proportion					2033:2042	the proportion	2029:2042	the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose)	2029:2135	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	5	78	theme	different	773:781	arg1	levels					783:788	different levels	773:788	different levels of B-domain deletion	773:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	9	79	theme	products	1383:1390	arg1	N-glycosylation					1318:1332	the N-glycosylation	1314:1332	the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines	1314:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	5	80	theme	substitution	653:664	arg1	therapy					666:672	A. Current substitution therapy	642:672	A. Current substitution therapy	642:672	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	3	81	theme	cell	371:374	arg1	line					376:379	cell line	371:379	cell line	371:379	Depending on cell line, significant differences to plasma-derived FVIII were observed.					
29888865	1	82	theme	Essentials	152:161	arg1	heterogeneity					177:189	Essentials Glycosylation heterogeneity	152:189	Essentials Glycosylation heterogeneity of recombinant proteins	152:213	Essentials Glycosylation heterogeneity of recombinant proteins affects pharmacokinetics and immunogenicity.					
29888865	3	83	theme	plasma-derived	409:422	arg1	FVIII					424:428	plasma-derived FVIII	409:428	plasma-derived FVIII	409:428	Depending on cell line, significant differences to plasma-derived FVIII were observed.					
29888865	5	84	gly	glycoprotein	590:601	arg1	glycoprotein					590:601	a plasma glycoprotein	581:601	a plasma glycoprotein	581:601	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	84	gly	glycoprotein	590:601	arg1	VIII					565:568	Background/Objective Human factor VIII	531:568	Background/Objective Human factor VIII (FVIII)	531:576	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	84	gly	glycoprotein	590:601	arg1	SUMMARY					522:528	SUMMARY	522:528	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.	522:810	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	84	gly	glycoprotein	590:601	arg1	defects					604:610	defects	604:610	defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion	604:809	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	11	85	theme	study	1657:1661	arg1	results					1642:1648	The results	1638:1648	The results	1638:1648	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	6	86	contain	has	885:887	arg1	modification					858:869	a post-translational protein modification	829:869	a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties	829:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	6	86	contain	has	885:887	arg1	Glycosylation					812:824	Glycosylation	812:824	Glycosylation	812:824	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	6	86	contain	has	885:887	arg2	influence					903:911	a substantial influence	889:911	a substantial influence on its physical, functional and antigenic properties	889:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	0	87	theme	recombinant	89:99	arg1	products					101:108	different recombinant products	79:108	different recombinant products	79:108	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	13	88	dep	sialylation	2107:2117	arg1	i.e.					2102:2105	i.e.	2102:2105	i.e.	2102:2105	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	10	89	theme	MS/MS	1576:1580	arg1	combination					1548:1558	a combination	1546:1558	a combination of MALDI-MS and MS/MS	1546:1580	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	10	89	theme	MS/MS	1576:1580	arg1	GC-MS					1583:1587	GC-MS	1583:1587	GC-MS	1583:1587	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	10	89	theme	MS/MS	1576:1580	arg1	technologies					1605:1616	UPLC-UV-MSE technologies	1593:1616	UPLC-UV-MSE technologies	1593:1616	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	9	90	theme	FVIII	1288:1292	arg1	biology					1294:1300	FVIII biology	1288:1300	FVIII biology	1288:1300	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	10	91	theme	MALDI-MS	1563:1570	arg1	combination					1548:1558	a combination	1546:1558	a combination of MALDI-MS and MS/MS	1546:1580	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	10	91	theme	MALDI-MS	1563:1570	arg1	GC-MS					1583:1587	GC-MS	1583:1587	GC-MS	1583:1587	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	10	91	theme	MALDI-MS	1563:1570	arg1	technologies					1605:1616	UPLC-UV-MSE technologies	1593:1616	UPLC-UV-MSE technologies	1593:1616	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	9	92	theme	cell	1421:1424	arg1	lines					1426:1430	CHO, BHK or HEK cell lines	1405:1430	lines	1426:1430	Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines.					
29888865	10	93	theme	UPLC-UV-MSE	1593:1603	arg1	technologies					1605:1616	UPLC-UV-MSE technologies	1593:1616	UPLC-UV-MSE technologies	1593:1616	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	14	94	theme	human	2416:2420	arg1	pdFVIII					2422:2428	human pdFVIII	2416:2428	human pdFVIII	2416:2428	rFVIII expressed in HEK cells showed the greatest glycomic differences to human pdFVIII.					
29888865	11	95	dep	study	1657:1661	arg1	detail					1663:1668	detail	1663:1668	detail	1663:1668	Results/Conclusion The results of our study detail the N-glycan repertoire of pdFVIII to an unprecedented level, and for the first time, provide evidence of N-glycolylneuraminic acid (NeuGc) found on pdFVIII.					
29888865	0	96	theme	N-glycosylation	23:37	arg1	comparison					9:18	In-depth comparison	0:18	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.	0:150	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	6	97	theme	post-translational	831:848	arg1	modification					858:869	a post-translational protein modification	829:869	a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties	829:964	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	6	97	theme	post-translational	831:848	arg1	Glycosylation					812:824	Glycosylation	812:824	Glycosylation	812:824	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	8	98	theme	coherent	1203:1210	arg1	model					1212:1216	a coherent model	1201:1216	a coherent model	1201:1216	However, the literature on FVIII glycosylation is inconsistent, preventing assembly into a coherent model.					
29888865	10	99	theme	Methods	1433:1439	arg1	samples					1447:1453	Methods FVIII samples	1433:1453	Methods FVIII samples	1433:1453	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	14	100	theme	greatest	2383:2390	arg1	differences					2401:2411	the greatest glycomic differences	2379:2411	the greatest glycomic differences	2379:2411	rFVIII expressed in HEK cells showed the greatest glycomic differences to human pdFVIII.					
29888865	0	101	theme	human	42:46	arg1	VIII					70:73	human plasma-derived factor VIII	42:73	human plasma-derived factor VIII	42:73	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	13	102	theme	recombinant	2166:2176	arg1	products					2178:2185	some recombinant products	2161:2185	some recombinant products	2161:2185	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29888865	0	103	theme	factor	63:68	arg1	VIII					70:73	human plasma-derived factor VIII	42:73	human plasma-derived factor VIII	42:73	In-depth comparison of N-glycosylation of human plasma-derived factor VIII and different recombinant products: from structure to clinical implications.					
29888865	12	104	theme	sample	1979:1984	arg1	content					1963:1969	the entire N-glycan content	1943:1969	the entire N-glycan content of each sample	1943:1984	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	12	105	theme	entire	1947:1952	arg1	content					1963:1969	the entire N-glycan content	1943:1969	the entire N-glycan content of each sample	1943:1984	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	6	106	from	modification	858:869	arg1	FVIII					874:878	FVIII	874:878	FVIII	874:878	Glycosylation is a post-translational protein modification in FVIII that has a substantial influence on its physical, functional and antigenic properties.					
29888865	10	107	theme	FVIII	1441:1445	arg1	samples					1447:1453	Methods FVIII samples	1433:1453	Methods FVIII samples	1433:1453	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	5	108	theme	Human	552:556	arg1	glycoprotein					590:601	a plasma glycoprotein	581:601	a plasma glycoprotein	581:601	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	108	theme	Human	552:556	arg1	FVIII					571:575	FVIII	571:575	FVIII	571:575	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	108	theme	Human	552:556	arg1	VIII					565:568	Background/Objective Human factor VIII	531:568	Background/Objective Human factor VIII (FVIII)	531:576	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	5	108	theme	Human	552:556	arg1	SUMMARY					522:528	SUMMARY	522:528	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.	522:810	SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion.					
29888865	10	109	theme	detailed	1486:1493	arg1	glycomic					1495:1502	head-to-head detailed glycomic	1473:1502	head-to-head detailed glycomic	1473:1502	Methods FVIII samples were subjected to head-to-head detailed glycomic and glycoproteomic characterization using a combination of MALDI-MS and MS/MS, GC-MS and UPLC-UV-MSE technologies.					
29888865	12	110	theme	expression	1924:1933	arg1	system					1935:1940	the expression system	1920:1940	the expression system	1920:1940	Although site-specific glycosylation of rFVIII proved consistent with pdFVIII regardless of the expression system, the entire N-glycan content of each sample appeared significantly different.					
29888865	13	111	theme	Gal	2328:2330	arg1	epitopes					2332:2339	Galα1,3 Gal epitopes	2320:2339	Galα1,3 Gal epitopes	2320:2339	Although the proportion of biologically important epitopes common to all samples (i.e. sialylation and high-mannose) varied between samples, some recombinant products expressed distinct and immunologically relevant epitopes, such as LacdiNAc (LDN), fucosylated LacdiNAc (FucLDN), NeuGc, LewisX/Y and Galα1,3 Gal epitopes.					
29773674	8	0	gly	glycosylated	1401:1412	arg1	regions					1425:1431	densely glycosylated mucin-like regions	1393:1431	densely glycosylated mucin-like regions	1393:1431	For neurocan and brevican, we identified densely glycosylated mucin-like regions in the extended domains.					
29773674	5	1	from	coverage	914:921	arg1	proteoglycans					970:982	proteoglycans	970:982	proteoglycans	970:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	2	2	theme	surface	380:386	arg1	receptors					388:396	cell surface receptors	375:396	cell surface receptors	375:396	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	9	3	from	O-glycosites	1522:1533	arg1	region					1551:1556	the extended region	1538:1556	the extended region	1538:1556	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	0	4	theme	High	63:66	arg1	Coverage					68:75	High Coverage	63:75	High Coverage	63:75	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	9	5	from	linker-glycosites	1489:1505	arg1	region					1551:1556	the extended region	1538:1556	the extended region	1538:1556	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	5	6	theme	peptides	958:965	arg1	coverage					914:921	sequence coverage	905:921	sequence coverage	905:921	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	5	6	theme	peptides	958:965	arg1	identification					927:940	identification	927:940	identification of glycosylated peptides in proteoglycans	927:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	2	7	theme	cell	375:378	arg1	receptors					388:396	cell surface receptors	375:396	cell surface receptors	375:396	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	6	8	theme	hyalectan	1067:1075	arg1	proteoglycans					1077:1089	the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans	1013:1089	the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans	1013:1089	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	0	9	theme	Site-specific	81:93	arg1	Identification					95:108	Site-specific Identification	81:108	Site-specific Identification	81:108	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	3	10	theme	mucin-type	558:567	arg1	classes					578:584	mucin-type O-glycan classes	558:584	mucin-type O-glycan classes	558:584	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	10	11	from	samples	1813:1819	arg1	analysis					1758:1765	routine analysis	1750:1765	routine analysis of proteoglycan glycosylation from biological samples	1750:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	10	11	from	samples	1813:1819	arg1	glycosylation					1783:1795	proteoglycan glycosylation	1770:1795	proteoglycan glycosylation from biological samples	1770:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	6	12	theme	proteoglycan	1036:1047	arg1	decorin					1049:1055	small leucine-rich proteoglycan decorin	1017:1055	small leucine-rich proteoglycan decorin	1017:1055	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	3	13	theme	O-glycan	569:576	arg1	classes					578:584	mucin-type O-glycan classes	558:584	mucin-type O-glycan classes	558:584	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	9	14	theme	domains	1591:1597	arg1	many					1600:1603	many	1600:1603	many	1600:1603	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	9	14	theme	domains	1591:1597	arg1	domains					1591:1597	the globular domains	1578:1597	the globular domains	1578:1597	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	5	15	theme	sequence	905:912	arg1	coverage					914:921	sequence coverage	905:921	sequence coverage	905:921	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	8	16	theme	glycosylated	1401:1412	arg1	regions					1425:1431	densely glycosylated mucin-like regions	1393:1431	densely glycosylated mucin-like regions	1393:1431	For neurocan and brevican, we identified densely glycosylated mucin-like regions in the extended domains.					
29773674	6	17	theme	LC-MS	1185:1189	arg1	methods					1191:1197	LC-MS methods	1185:1197	LC-MS methods easily implemented on instruments widely used in proteomics laboratories	1185:1270	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	2	18	theme	regulated	294:302	arg1	manner					304:309	a spatially and temporally regulated manner	267:309	a spatially and temporally regulated manner	267:309	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	9	19	gly	linker-glycosites	1489:1505	arg2	linker-glycosites					1489:1505	50 linker-glycosites	1486:1505	50 linker-glycosites	1486:1505	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	1	20	theme	animal	185:190	arg1	tissues					192:198	all animal tissues	181:198	all animal tissues	181:198	Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles.					
29773674	3	21	theme	high	488:491	arg1	degree					493:498	the high degree	484:498	the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes	484:584	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	10	22	theme	bioinformatics	1709:1722	arg1	approach					1724:1731	an LC-MS and bioinformatics approach	1696:1731	approach	1724:1731	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	10	23	theme	proteoglycan	1770:1781	arg1	glycosylation					1783:1795	proteoglycan glycosylation	1770:1795	proteoglycan glycosylation from biological samples	1770:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	6	24	theme	proteoglycans	1165:1177	arg1	glycosylation					1142:1154	glycosylation	1142:1154	glycosylation of these proteoglycans	1142:1177	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	0	25	link	Glycosaminoglycan-linked	113:136	arg1	Peptides					138:145	Glycosaminoglycan-linked Peptides	113:145	Glycosaminoglycan-linked Peptides	113:145	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	2	26	theme	key	407:409	arg1	roles					411:415	key roles	407:415	key roles	407:415	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	0	27	theme	Deep	0:3	arg1	Sequencing					5:14	Deep Sequencing	0:14	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.	0:146	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	6	28	theme	decorin	1049:1055	arg1	proteoglycans					1077:1089	the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans	1013:1089	the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans	1013:1089	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	5	29	from	proteoglycans	970:982	arg1	coverage					914:921	sequence coverage	905:921	sequence coverage	905:921	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	5	29	from	proteoglycans	970:982	arg1	identification					927:940	identification	927:940	identification of glycosylated peptides in proteoglycans	927:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	3	30	theme	peptide	591:597	arg1	coverage					608:615	the peptide sequence coverage	587:615	the peptide sequence coverage of complex proteoglycans	587:640	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	5	31	from	identification	927:940	arg1	proteoglycans					970:982	proteoglycans	970:982	proteoglycans	970:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	0	32	theme	Complex	19:25	arg1	Proteoglycans					27:39	Complex Proteoglycans	19:39	Complex Proteoglycans	19:39	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	0	33	dep	Sequencing	5:14	arg1	Strategy					50:57	A Novel Strategy	42:57	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.	0:146	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	0	34	theme	Peptides	138:145	arg1	Coverage					68:75	High Coverage	63:75	High Coverage	63:75	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	0	34	theme	Peptides	138:145	arg1	Identification					95:108	Site-specific Identification	81:108	Site-specific Identification	81:108	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	10	35	from	role	1837:1840	arg1	pathophysiology					1845:1859	pathophysiology	1845:1859	pathophysiology	1845:1859	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	4	36	theme	specific	791:798	arg1	glycosylation					800:812	proteoglycan site specific glycosylation	773:812	proteoglycan site specific glycosylation	773:812	As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes.					
29773674	5	37	from	peptides	958:965	arg1	proteoglycans					970:982	proteoglycans	970:982	proteoglycans	970:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	2	38	theme	other	443:447	arg1	matrices					463:470	other extracellular matrices	443:470	other extracellular matrices	443:470	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	2	39	theme	extracellular	449:461	arg1	matrices					463:470	other extracellular matrices	443:470	other extracellular matrices	443:470	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	6	40	theme	leucine-rich	1023:1034	arg1	decorin					1049:1055	small leucine-rich proteoglycan decorin	1017:1055	small leucine-rich proteoglycan decorin	1017:1055	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	0	41	theme	Glycosaminoglycan-linked	113:136	arg1	Peptides					138:145	Glycosaminoglycan-linked Peptides	113:145	Glycosaminoglycan-linked Peptides	113:145	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	3	42	theme	mass	673:676	arg1	methods					708:714	standard mass spectrometry-based proteomics methods	664:714	standard mass spectrometry-based proteomics methods	664:714	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	8	43	theme	extended	1440:1447	arg1	domains					1449:1455	the extended domains	1436:1455	the extended domains	1436:1455	For neurocan and brevican, we identified densely glycosylated mucin-like regions in the extended domains.					
29773674	6	44	theme	proteomics	1248:1257	arg1	laboratories					1259:1270	proteomics laboratories	1248:1270	proteomics laboratories	1248:1270	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	10	45	theme	routine	1750:1756	arg1	analysis					1758:1765	routine analysis	1750:1765	routine analysis of proteoglycan glycosylation from biological samples	1750:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	6	46	theme	small	1017:1021	arg1	decorin					1049:1055	small leucine-rich proteoglycan decorin	1017:1055	small leucine-rich proteoglycan decorin	1017:1055	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	3	47	theme	spectrometry-based	678:695	arg1	methods					708:714	standard mass spectrometry-based proteomics methods	664:714	standard mass spectrometry-based proteomics methods	664:714	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	9	48	theme	globular	1582:1589	arg1	domains					1591:1597	the globular domains	1578:1597	the globular domains	1578:1597	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	10	49	theme	biological	1802:1811	arg1	samples					1813:1819	biological samples	1802:1819	biological samples	1802:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	0	50	theme	Proteoglycans	27:39	arg1	Sequencing					5:14	Deep Sequencing	0:14	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.	0:146	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	9	51	theme	extended	1542:1549	arg1	region					1551:1556	the extended region	1538:1556	the extended region	1538:1556	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	3	52	theme	complex	620:626	arg1	proteoglycans					628:640	complex proteoglycans	620:640	complex proteoglycans	620:640	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	2	53	theme	basement	420:427	arg1	membranes					429:437	basement membranes	420:437	basement membranes	420:437	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	0	54	theme	Novel	44:48	arg1	Strategy					50:57	A Novel Strategy	42:57	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.	0:146	Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.					
29773674	9	55	gly	N-glycosites	1562:1573	arg2	N-glycosites					1562:1573	N-glycosites	1562:1573	N-glycosites	1562:1573	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	5	56	gly	glycosylated	945:956	arg1	peptides					958:965	glycosylated peptides	945:965	glycosylated peptides in proteoglycans	945:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29773674	3	57	theme	standard	664:671	arg1	methods					708:714	standard mass spectrometry-based proteomics methods	664:714	standard mass spectrometry-based proteomics methods	664:714	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	3	58	theme	sequence	599:606	arg1	coverage					608:615	the peptide sequence coverage	587:615	the peptide sequence coverage of complex proteoglycans	587:640	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	10	59	from	analysis	1758:1765	arg1	samples					1813:1819	biological samples	1802:1819	biological samples	1802:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	4	60	theme	little	739:744	arg1	information					746:756	little information	739:756	little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes	739:861	As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes.					
29773674	9	61	gly	O-glycosites	1522:1533	arg2	O-glycosites					1522:1533	mucin-type O-glycosites	1511:1533	mucin-type O-glycosites	1511:1533	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	6	62	gly	glycosylation	1142:1154	arg1	proteoglycans					1165:1177	these proteoglycans	1159:1177	these proteoglycans	1159:1177	We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories.					
29773674	4	63	theme	pathological	840:851	arg1	processes					853:861	normal and pathological processes	829:861	normal and pathological processes	829:861	As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes.					
29773674	2	64	theme	growth	356:361	arg1	factors					363:369	growth factors	356:369	growth factors	356:369	Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.					
29773674	3	65	theme	proteoglycans	628:640	arg1	coverage					608:615	the peptide sequence coverage	587:615	the peptide sequence coverage of complex proteoglycans	587:640	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	4	66	theme	normal	829:834	arg1	processes					853:861	normal and pathological processes	829:861	normal and pathological processes	829:861	As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes.					
29773674	3	67	theme	proteomics	697:706	arg1	methods					708:714	standard mass spectrometry-based proteomics methods	664:714	standard mass spectrometry-based proteomics methods	664:714	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	7	68	gly	linker-glycosite	1302:1317	arg2	linker-glycosite					1302:1317	the linker-glycosite and three N-glycosylation sites	1298:1349	linker-glycosite	1302:1317	For decorin, we assigned the linker-glycosite and three N-glycosylation sites.					
29773674	3	69	theme	glycosylation	503:515	arg1	degree					493:498	the high degree	484:498	the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes	484:584	Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods.					
29773674	9	70	theme	mucin-type	1511:1520	arg1	O-glycosites					1522:1533	mucin-type O-glycosites	1511:1533	mucin-type O-glycosites	1511:1533	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	7	71	theme	N-glycosylation	1329:1343	arg1	sites					1345:1349	the linker-glycosite and three N-glycosylation sites	1298:1349	sites	1345:1349	For decorin, we assigned the linker-glycosite and three N-glycosylation sites.					
29773674	1	72	dep	critical	209:216	arg1	physiological					233:245	physiological	233:245	physiological	233:245	Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles.					
29773674	1	72	dep	critical	209:216	arg1	multifaceted					219:230	multifaceted	219:230	multifaceted	219:230	Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles.					
29773674	1	73	theme	critical	209:216	arg1	roles					247:251	critical, multifaceted, physiological roles	209:251	critical, multifaceted, physiological roles	209:251	Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles.					
29773674	8	74	theme	mucin-like	1414:1423	arg1	regions					1425:1431	densely glycosylated mucin-like regions	1393:1431	densely glycosylated mucin-like regions	1393:1431	For neurocan and brevican, we identified densely glycosylated mucin-like regions in the extended domains.					
29773674	10	75	theme	glycosylation	1783:1795	arg1	analysis					1758:1765	routine analysis	1750:1765	routine analysis of proteoglycan glycosylation from biological samples	1750:1819	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	7	76	gly	N-glycosylation	1329:1343	arg2	sites					1345:1349	the linker-glycosite and three N-glycosylation sites	1298:1349	sites	1345:1349	For decorin, we assigned the linker-glycosite and three N-glycosylation sites.					
29773674	7	76	gly	N-glycosylation	1329:1343	arg2	three					1323:1327	three	1323:1327	three	1323:1327	For decorin, we assigned the linker-glycosite and three N-glycosylation sites.					
29773674	10	77	theme	LC-MS	1699:1703	arg1	approach					1724:1731	an LC-MS and bioinformatics approach	1696:1731	approach	1724:1731	Most importantly, we demonstrate an LC-MS and bioinformatics approach that will enable routine analysis of proteoglycan glycosylation from biological samples to assess their role in pathophysiology.					
29773674	9	78	from	N-glycosites	1562:1573	arg1	region					1551:1556	the extended region	1538:1556	the extended region	1538:1556	For aggrecan, we identified 50 linker-glycosites and mucin-type O-glycosites in the extended region and N-glycosites in the globular domains, many of which are novel and have not been observed previously.					
29773674	5	79	theme	glycosylated	945:956	arg1	peptides					958:965	glycosylated peptides	945:965	glycosylated peptides in proteoglycans	945:982	Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans.					
29878199	2	0	with	combination	249:259	arg1	technologies					299:310	high-throughput functional-omics technologies	266:310	high-throughput functional-omics technologies	266:310	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	4	1	theme	known	671:675	arg1	defect					685:690	a known genetic defect	669:690	a known genetic defect in 15 individuals	669:708	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	2	2	theme	inheritance	437:447	arg1	families					415:422	singleton families	405:422	singleton families of recessive inheritance	405:447	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	8	3	theme	complete	1216:1223	arg1	loss					1225:1228	a complete loss	1214:1228	a complete loss of SLC10A7 mRNA	1214:1244	The two other individuals showed a complete loss of SLC10A7 mRNA.					
29878199	2	4	theme	causative	363:371	arg1	variants					381:388	causative genetic variants	363:388	causative genetic variants	363:388	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	6	5	located	found	979:983	arg2	mutations					964:972	compound heterozygous mutations	942:972	compound heterozygous mutations	942:972	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	6	5	located	found	979:983	arg1	SLC10A7					988:994	SLC10A7	988:994	SLC10A7	988:994	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	6	5	located	found	979:983	arg1	siblings					932:939	two siblings	928:939	two siblings	928:939	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	6	5	located	found	979:983	arg1	gene					999:1002	a gene	997:1002	a gene of unknown function in human	997:1031	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	11	6	theme	alizarin	1476:1483	arg1	staining					1489:1496	alizarin red staining	1476:1496	alizarin red staining of calcium deposits in zebrafish morphants	1476:1539	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	8	7	theme	mRNA	1241:1244	arg1	loss					1225:1228	a complete loss	1214:1228	a complete loss of SLC10A7 mRNA	1214:1244	The two other individuals showed a complete loss of SLC10A7 mRNA.					
29878199	5	8	theme	genetic	734:740	arg1	factors					742:748	additional genetic factors	723:748	additional genetic factors	723:748	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	1	9	theme	Mendelian	224:232	arg1	disorders					234:242	Mendelian disorders	224:242	Mendelian disorders	224:242	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	4	10	with	Combination	608:618	arg1	sequencing					637:646	whole-exome sequencing	625:646	whole-exome sequencing in 31 cases	625:658	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	14	11	theme	extracellular	1959:1971	arg1	matrix					1973:1978	the extracellular matrix	1955:1978	the extracellular matrix	1955:1978	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	7	12	from	domain	1098:1103	arg1	site					1143:1146	a splice acceptor site	1125:1146	a splice acceptor site resulting in skipping of exon 9	1125:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	7	13	theme	splice	1127:1132	arg1	site					1143:1146	a splice acceptor site	1125:1146	a splice acceptor site resulting in skipping of exon 9	1125:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	2	14	theme	functional-omics	282:297	arg1	technologies					299:310	high-throughput functional-omics technologies	266:310	high-throughput functional-omics technologies	266:310	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	6	15	theme	function	1015:1022	arg1	SLC10A7					988:994	SLC10A7	988:994	SLC10A7	988:994	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	6	15	theme	function	1015:1022	arg1	gene					999:1002	a gene	997:1002	a gene of unknown function in human	997:1031	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	9	16	theme	skeletal	1309:1316	arg1	dysplasia					1318:1326	skeletal dysplasia	1309:1326	skeletal dysplasia	1309:1326	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	7	17	theme	exon	1173:1176	arg1	skipping					1161:1168	skipping	1161:1168	skipping of exon 9	1161:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	12	18	theme	glycoproteins	1691:1703	arg1	defect					1711:1716	a defect	1709:1716	a defect in post-Golgi transport of glycoproteins to the cell membrane	1709:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	12	18	theme	glycoproteins	1691:1703	arg1	mislocalization					1672:1686	intracellular mislocalization	1658:1686	intracellular mislocalization of glycoproteins	1658:1703	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	11	19	theme	bone	1570:1573	arg1	mineralization					1575:1588	bone mineralization	1570:1588	bone mineralization	1570:1588	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	5	20	theme	glycomics	789:797	arg1	data					799:802	the plasma glycomics data	778:802	the plasma glycomics data	778:802	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	12	21	theme	affected	1630:1637	arg1	individuals					1639:1649	affected individuals	1630:1649	affected individuals	1630:1649	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	14	22	gly	glycoproteins	1938:1950	arg1	glycoproteins					1938:1950	glycoproteins	1938:1950	glycoproteins	1938:1950	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	9	23	theme	decreased	1333:1341	arg1	density					1356:1362	decreased bone mineral density	1333:1362	decreased bone mineral density compatible with osteoporosis	1333:1391	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	0	24	theme	bone	76:79	arg1	mineralization					81:94	bone mineralization	76:94	bone mineralization	76:94	Integrating glycomics and genomics uncovers SLC10A7 as essential factor for bone mineralization by regulating post-Golgi protein transport and glycosylation.					
29878199	9	25	theme	mineral	1348:1354	arg1	density					1356:1362	decreased bone mineral density	1333:1362	decreased bone mineral density compatible with osteoporosis	1333:1391	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	9	26	theme	compatible	1364:1373	arg1	density					1356:1362	decreased bone mineral density	1333:1362	decreased bone mineral density compatible with osteoporosis	1333:1391	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	12	27	theme	cell	1766:1769	arg1	membrane					1771:1778	the cell membrane	1762:1778	the cell membrane	1762:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	9	28	with	compatible	1364:1373	arg1	osteoporosis					1380:1391	osteoporosis	1380:1391	osteoporosis	1380:1391	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	0	29	theme	post-Golgi	110:119	arg1	transport					129:137	post-Golgi protein transport	110:137	post-Golgi protein transport	110:137	Integrating glycomics and genomics uncovers SLC10A7 as essential factor for bone mineralization by regulating post-Golgi protein transport and glycosylation.					
29878199	11	30	theme	calcium	1501:1507	arg1	deposits					1509:1516	calcium deposits	1501:1516	calcium deposits	1501:1516	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	12	31	from	studies	1604:1610	arg1	fibroblasts					1615:1625	fibroblasts	1615:1625	fibroblasts of affected individuals	1615:1649	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	11	32	from	reduction	1557:1565	arg1	mineralization					1575:1588	bone mineralization	1570:1588	bone mineralization	1570:1588	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	5	33	theme	type	909:912	arg1	N-glycans					914:922	hybrid type N-glycans	902:922	hybrid type N-glycans	902:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	6	34	theme	heterozygous	951:962	arg1	mutations					964:972	compound heterozygous mutations	942:972	compound heterozygous mutations	942:972	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	14	35	theme	glycoproteins	1938:1950	arg1	role					1881:1884	an important role	1868:1884	an important role for SLC10A7 in bone mineralization	1868:1919	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	14	35	theme	glycoproteins	1938:1950	arg1	transport					1925:1933	transport	1925:1933	transport of glycoproteins to the extracellular matrix	1925:1978	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	3	36	theme	Golgi	494:498	arg1	glycosylation					500:512	abnormal Golgi glycosylation	485:512	abnormal Golgi glycosylation	485:512	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	12	37	theme	biology	1596:1602	arg1	studies					1604:1610	Cell biology studies	1591:1610	Cell biology studies in fibroblasts of affected individuals	1591:1649	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	2	38	theme	recessive	427:435	arg1	inheritance					437:447	recessive inheritance	427:447	recessive inheritance	427:447	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	5	39	theme	glycomics	879:887	arg1	signature					889:897	a unique glycomics signature	870:897	a unique glycomics signature of hybrid type N-glycans	870:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	14	40	theme	important	1871:1879	arg1	role					1881:1884	an important role	1868:1884	an important role for SLC10A7 in bone mineralization	1868:1919	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	4	41	theme	whole-exome	625:635	arg1	sequencing					637:646	whole-exome sequencing	625:646	whole-exome sequencing in 31 cases	625:658	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	12	42	gly	glycoproteins	1745:1757	arg1	glycoproteins					1745:1757	glycoproteins	1745:1757	glycoproteins	1745:1757	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	12	43	from	mislocalization	1672:1686	arg1	transport					1732:1740	post-Golgi transport	1721:1740	post-Golgi transport of glycoproteins to the cell membrane	1721:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	14	44	theme	combined	1845:1852	arg1	data					1854:1857	Our combined data	1841:1857	Our combined data	1841:1857	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	5	45	theme	unique	872:877	arg1	signature					889:897	a unique glycomics signature	870:897	a unique glycomics signature of hybrid type N-glycans	870:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	6	46	from	gene	999:1002	arg1	human					1027:1031	human	1027:1031	human	1027:1031	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	3	47	theme	total	580:584	arg1	glycoproteins					593:605	total plasma glycoproteins	580:605	total plasma glycoproteins	580:605	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	11	48	theme	red	1485:1487	arg1	staining					1489:1496	alizarin red staining	1476:1496	alizarin red staining of calcium deposits in zebrafish morphants	1476:1539	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	5	49	theme	hierarchical	751:762	arg1	clustering					764:773	hierarchical clustering	751:773	hierarchical clustering of the plasma glycomics data	751:802	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	2	50	theme	genetic	373:379	arg1	variants					381:388	causative genetic variants	363:388	causative genetic variants	363:388	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	4	51	theme	genetic	677:683	arg1	defect					685:690	a known genetic defect	669:690	a known genetic defect in 15 individuals	669:708	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	1	52	contain	have	181:184	arg1	methodologies					167:179	Genomics methodologies	158:179	Genomics methodologies	158:179	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	1	52	contain	have	181:184	arg2	elucidation					209:219	significantly improved elucidation	186:219	significantly improved elucidation of Mendelian disorders	186:242	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	10	53	theme	deficient	1442:1450	arg1	zebrafish					1452:1460	SLC10A7 deficient zebrafish	1434:1460	SLC10A7 deficient zebrafish	1434:1460	The patients' phenotype was mirrored in SLC10A7 deficient zebrafish.					
29878199	1	54	theme	improved	200:207	arg1	elucidation					209:219	significantly improved elucidation	186:219	significantly improved elucidation of Mendelian disorders	186:242	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	14	55	from	role	1881:1884	arg1	mineralization					1906:1919	bone mineralization	1901:1919	bone mineralization	1901:1919	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	5	56	theme	additional	723:732	arg1	factors					742:748	additional genetic factors	723:748	additional genetic factors	723:748	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	8	57	theme	SLC10A7	1233:1239	arg1	mRNA					1241:1244	SLC10A7 mRNA	1233:1244	SLC10A7 mRNA	1233:1244	The two other individuals showed a complete loss of SLC10A7 mRNA.					
29878199	2	58	dep	identification	328:341	arg1	the					324:326	the	324:326	the	324:326	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	5	59	theme	data	799:802	arg1	clustering					764:773	hierarchical clustering	751:773	hierarchical clustering of the plasma glycomics data	751:802	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	12	60	gly	glycoproteins	1691:1703	arg1	glycoproteins					1691:1703	glycoproteins	1691:1703	glycoproteins	1691:1703	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	1	61	theme	disorders	234:242	arg1	elucidation					209:219	significantly improved elucidation	186:219	significantly improved elucidation of Mendelian disorders	186:242	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	12	62	theme	post-Golgi	1721:1730	arg1	transport					1732:1740	post-Golgi transport	1721:1740	post-Golgi transport of glycoproteins to the cell membrane	1721:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	3	63	with	individuals	468:478	arg1	glycosylation					500:512	abnormal Golgi glycosylation	485:512	abnormal Golgi glycosylation	485:512	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	9	64	theme	amelogenesis	1284:1295	arg1	imperfecta					1297:1306	amelogenesis imperfecta	1284:1306	amelogenesis imperfecta	1284:1306	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	13	65	dep	yeast	1796:1800	arg1	contrast					1784:1791	contrast	1784:1791	contrast	1784:1791	In contrast to yeast, human SLC10A7 localized to the Golgi.					
29878199	11	66	from	staining	1489:1496	arg1	morphants					1531:1539	zebrafish morphants	1521:1539	zebrafish morphants	1521:1539	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	2	67	theme	high-throughput	266:280	arg1	technologies					299:310	high-throughput functional-omics technologies	266:310	high-throughput functional-omics technologies	266:310	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	6	68	theme	unknown	1007:1013	arg1	function					1015:1022	unknown function	1007:1022	unknown function	1007:1022	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	7	69	theme	acceptor	1134:1141	arg1	site					1143:1146	a splice acceptor site	1125:1146	a splice acceptor site resulting in skipping of exon 9	1125:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	5	70	theme	plasma	782:787	arg1	data					799:802	the plasma glycomics data	778:802	the plasma glycomics data	778:802	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	7	71	theme	transmembrane	1084:1096	arg1	domain					1098:1103	transmembrane domain 4	1084:1105	transmembrane domain 4	1084:1105	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	12	72	theme	individuals	1639:1649	arg1	fibroblasts					1615:1625	fibroblasts	1615:1625	fibroblasts of affected individuals	1615:1649	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	11	73	theme	strong	1550:1555	arg1	reduction					1557:1565	a strong reduction	1548:1565	a strong reduction in bone mineralization	1548:1588	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	5	74	theme	N-glycans	914:922	arg1	signature					889:897	a unique glycomics signature	870:897	a unique glycomics signature of hybrid type N-glycans	870:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	1	75	theme	Genomics	158:165	arg1	methodologies					167:179	Genomics methodologies	158:179	Genomics methodologies	158:179	Genomics methodologies have significantly improved elucidation of Mendelian disorders.					
29878199	12	76	theme	intracellular	1658:1670	arg1	mislocalization					1672:1686	intracellular mislocalization	1658:1686	intracellular mislocalization of glycoproteins	1658:1703	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	5	77	theme	patients	849:856	arg1	subgroup					832:839	a subgroup	830:839	a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans	830:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	9	78	theme	bone	1343:1346	arg1	density					1356:1362	decreased bone mineral density	1333:1362	decreased bone mineral density compatible with osteoporosis	1333:1391	The patients' phenotype consisted of amelogenesis imperfecta, skeletal dysplasia, and decreased bone mineral density compatible with osteoporosis.					
29878199	3	79	gly	glycosylation	500:512	arg1	individuals					468:478	99 individuals	465:478	99 individuals	465:478	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	7	80	theme	missense	1051:1058	arg1	mutation					1060:1067	a missense mutation	1049:1067	a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9	1049:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	13	81	theme	human	1803:1807	arg1	SLC10A7					1809:1815	human SLC10A7	1803:1815	human SLC10A7	1803:1815	In contrast to yeast, human SLC10A7 localized to the Golgi.					
29878199	4	82	from	defect	685:690	arg1	individuals					698:708	15 individuals	695:708	15 individuals	695:708	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	2	83	theme	variants	381:388	arg1	confirmation					347:358	confirmation	347:358	confirmation	347:358	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	2	83	theme	variants	381:388	arg1	identification					328:341	identification	328:341	identification	328:341	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	3	84	gly	glycoproteins	593:605	arg1	glycoproteins					593:605	total plasma glycoproteins	580:605	total plasma glycoproteins	580:605	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	11	85	theme	deposits	1509:1516	arg1	staining					1489:1496	alizarin red staining	1476:1496	alizarin red staining of calcium deposits in zebrafish morphants	1476:1539	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	0	86	theme	protein	121:127	arg1	transport					129:137	post-Golgi protein transport	110:137	post-Golgi protein transport	110:137	Integrating glycomics and genomics uncovers SLC10A7 as essential factor for bone mineralization by regulating post-Golgi protein transport and glycosylation.					
29878199	3	87	theme	individuals	468:478	arg1	cohort					455:460	a cohort	453:460	a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved	453:540	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	3	87	theme	individuals	468:478	arg1	individuals					468:478	99 individuals	465:478	99 individuals	465:478	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	3	87	theme	individuals	468:478	arg1	47					515:516	47	515:516	47	515:516	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	5	88	theme	hybrid	902:907	arg1	N-glycans					914:922	hybrid type N-glycans	902:922	hybrid type N-glycans	902:922	To identify additional genetic factors, hierarchical clustering of the plasma glycomics data was done, which indicated a subgroup of four patients that shared a unique glycomics signature of hybrid type N-glycans.					
29878199	14	89	theme	bone	1901:1904	arg1	mineralization					1906:1919	bone mineralization	1901:1919	bone mineralization	1901:1919	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29878199	11	90	theme	zebrafish	1521:1529	arg1	morphants					1531:1539	zebrafish morphants	1521:1539	zebrafish morphants	1521:1539	Furthermore, alizarin red staining of calcium deposits in zebrafish morphants showed a strong reduction in bone mineralization.					
29878199	3	91	theme	glycomics	543:551	arg1	profiling					553:561	glycomics profiling	543:561	glycomics profiling	543:561	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	4	92	from	sequencing	637:646	arg1	cases					654:658	31 cases	651:658	31 cases	651:658	Combination with whole-exome sequencing in 31 cases revealed a known genetic defect in 15 individuals.					
29878199	12	93	from	defect	1711:1716	arg1	transport					1732:1740	post-Golgi transport	1721:1740	post-Golgi transport of glycoproteins to the cell membrane	1721:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	6	94	theme	compound	942:949	arg1	mutations					964:972	compound heterozygous mutations	942:972	compound heterozygous mutations	942:972	In two siblings, compound heterozygous mutations were found in SLC10A7, a gene of unknown function in human.					
29878199	12	95	theme	glycoproteins	1745:1757	arg1	transport					1732:1740	post-Golgi transport	1721:1740	post-Golgi transport of glycoproteins to the cell membrane	1721:1778	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	0	96	theme	essential	55:63	arg1	factor					65:70	essential factor	55:70	essential factor for bone mineralization	55:94	Integrating glycomics and genomics uncovers SLC10A7 as essential factor for bone mineralization by regulating post-Golgi protein transport and glycosylation.					
29878199	12	97	theme	Cell	1591:1594	arg1	studies					1604:1610	Cell biology studies	1591:1610	Cell biology studies in fibroblasts of affected individuals	1591:1649	Cell biology studies in fibroblasts of affected individuals showed intracellular mislocalization of glycoproteins and a defect in post-Golgi transport of glycoproteins to the cell membrane.					
29878199	3	98	theme	abnormal	485:492	arg1	glycosylation					500:512	abnormal Golgi glycosylation	485:512	abnormal Golgi glycosylation	485:512	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	3	99	theme	plasma	586:591	arg1	glycoproteins					593:605	total plasma glycoproteins	580:605	total plasma glycoproteins	580:605	In a cohort of 99 individuals with abnormal Golgi glycosylation, 47 of which being unsolved, glycomics profiling was performed of total plasma glycoproteins.					
29878199	10	100	theme	SLC10A7	1434:1440	arg1	zebrafish					1452:1460	SLC10A7 deficient zebrafish	1434:1460	SLC10A7 deficient zebrafish	1434:1460	The patients' phenotype was mirrored in SLC10A7 deficient zebrafish.					
29878199	2	101	theme	singleton	405:413	arg1	families					415:422	singleton families	405:422	singleton families of recessive inheritance	405:447	The combination with high-throughput functional-omics technologies potentiates the identification and confirmation of causative genetic variants, especially in singleton families of recessive inheritance.					
29878199	8	102	theme	other	1189:1193	arg1	individuals					1195:1205	The two other individuals	1181:1205	The two other individuals	1181:1205	The two other individuals showed a complete loss of SLC10A7 mRNA.					
29878199	7	103	from	mutation	1113:1120	arg1	site					1143:1146	a splice acceptor site	1125:1146	a splice acceptor site resulting in skipping of exon 9	1125:1178	These included a missense mutation that disrupted transmembrane domain 4 and a mutation in a splice acceptor site resulting in skipping of exon 9.					
29878199	14	104	from	transport	1925:1933	arg1	mineralization					1906:1919	bone mineralization	1901:1919	bone mineralization	1901:1919	Our combined data indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.					
29632412	3	0	theme	several	444:450	arg1	observations					462:473	several important observations	444:473	several important observations	444:473	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	1	theme	glycosylation	739:751	arg1	motifs					758:763	traditional glycosylation site motifs	727:763	traditional glycosylation site motifs	727:763	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	2	dep	strains	1017:1023	arg1	v					990:990	v	990:990	v	990:990	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	3	theme	immune	140:145	arg1	system					147:152	the immune system	136:152	the immune system	136:152	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	3	4	theme	site	753:756	arg1	motifs					758:763	traditional glycosylation site motifs	727:763	traditional glycosylation site motifs	727:763	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	5	theme	glycans	929:935	arg1	absence					918:924	the absence	914:924	the absence of glycans containing the bisecting GlcNAc on murine IgGs	914:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	6	theme	Collaborative	270:282	arg1	resource					303:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	7	theme	mouse	506:510	arg1	strains					512:518	mouse strains	506:518	mouse strains	506:518	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	8	theme	murine	972:977	arg1	IgGs					979:982	murine IgGs	972:982	murine IgGs	972:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	9	theme	glycome	480:486	arg1	variation					488:496	(i) glycome variation	476:496	(i) glycome variation between mouse strains	476:518	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	10	theme	traditional	727:737	arg1	motifs					758:763	traditional glycosylation site motifs	727:763	traditional glycosylation site motifs	727:763	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	11	theme	system	147:152	arg1	function					124:131	function	124:131	function of the immune system	124:152	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	3	12	theme	glycosylation	1079:1091	arg1	effects					1068:1074	effects	1068:1074	effects of glycosylation on IgG function	1068:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	13	theme	genetic	632:638	arg1	loci					640:643	five genetic loci	627:643	five genetic loci	627:643	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	14	theme	bisecting	952:960	arg1	GlcNAc					962:967	the bisecting GlcNAc	948:967	the bisecting GlcNAc on murine IgGs	948:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	15	theme	Immunoglobulin	70:83	arg1	IgG					88:90	IgG	88:90	IgG	88:90	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	1	15	theme	Immunoglobulin	70:83	arg1	G					85:85	Immunoglobulin G	70:85	Immunoglobulin G (IgG) glycosylation	70:105	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	3	16	theme	bisecting	798:806	arg1	GlcNAc					829:834	GlcNAc	829:834	GlcNAc	829:834	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	16	theme	bisecting	798:806	arg1	N-acetylglucosamine					808:826	(iv) bisecting N-acetylglucosamine	793:826	(iv) bisecting N-acetylglucosamine (GlcNAc)	793:835	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	17	theme	variation	353:361	arg1	variation					353:361	the common genetic variation	334:361	the common genetic variation of the mouse	334:374	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	2	17	theme	variation	353:361	arg1	%					329:329	90%	327:329	90% of the common genetic variation of the mouse	327:374	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	1	18	theme	G	85:85	arg1	glycosylation					93:105	Immunoglobulin G (IgG) glycosylation	70:105	Immunoglobulin G (IgG) glycosylation	70:105	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	3	19	from	GlcNAc	962:967	arg1	IgGs					979:982	murine IgGs	972:982	murine IgGs	972:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	20	theme	genetic	345:351	arg1	variation					353:361	the common genetic variation	334:361	the common genetic variation of the mouse	334:374	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	21	contain	containing	937:946	arg2	GlcNAc					962:967	the bisecting GlcNAc	948:967	the bisecting GlcNAc on murine IgGs	948:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	21	contain	containing	937:946	arg1	glycans					929:935	glycans	929:935	glycans containing the bisecting GlcNAc on murine IgGs	929:982	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	22	from	effects	1068:1074	arg1	function					1100:1107	IgG function	1096:1107	IgG function	1096:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	23	theme	CC	291:292	arg1	resource					303:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	24	gly	glycosylation	739:751	arg2	site					753:756	traditional glycosylation site motifs	727:763	traditional glycosylation site motifs	727:763	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	24	gly	glycosylation	739:751	arg2	motifs					758:763	traditional glycosylation site motifs	727:763	traditional glycosylation site motifs	727:763	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	25	theme	common	338:343	arg1	variation					353:361	the common genetic variation	334:361	the common genetic variation of the mouse	334:374	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	26	theme	mouse	1011:1015	arg1	models					1048:1053	optimal animal models	1033:1053	optimal animal models for studying effects of glycosylation on IgG function	1033:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	26	theme	mouse	1011:1015	arg1	strains					1017:1023	(v) common laboratory mouse strains	989:1023	(v) common laboratory mouse strains	989:1023	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	27	theme	several	853:859	arg1	strains					861:867	several strains	853:867	several strains	853:867	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	28	theme	genetic	163:169	arg1	factors					189:195	the genetic and environmental factors	159:195	the genetic and environmental factors that underlie its inter-individual variability	159:242	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	0	29	theme	genetic	14:20	arg1	control					22:28	genetic control	14:28	genetic control	14:28	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	1	30	gly	glycosylation	93:105	arg1	system					147:152	the immune system	136:152	the immune system	136:152	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	3	31	dep	variants	710:717	arg1	made					439:442	made	439:442	made several important observations	439:473	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	32	theme	Cross	284:288	arg1	resource					303:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	33	theme	IgG	1096:1098	arg1	function					1100:1107	IgG function	1096:1107	IgG function	1096:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	34	theme	CC	424:425	arg1	strains					427:433	95 CC strains	421:433	95 CC strains	421:433	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	35	gly	glycosylation	1079:1091	arg1	IgG					1096:1098	IgG function	1096:1107	IgG function	1096:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	36	theme	environmental	175:187	arg1	factors					189:195	the genetic and environmental factors	159:195	the genetic and environmental factors that underlie its inter-individual variability	159:242	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29632412	2	37	theme	mouse	370:374	arg1	variation					353:361	the common genetic variation	334:361	the common genetic variation of the mouse	334:374	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	38	theme	IgG	394:396	arg1	composition					406:416	the IgG glycome composition	390:416	the IgG glycome composition of 95 CC strains	390:433	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	39	dep	N-acetylglucosamine	808:826	arg1	iv					794:795	iv	794:795	iv	794:795	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	40	theme	laboratory	1000:1009	arg1	models					1048:1053	optimal animal models	1033:1053	optimal animal models for studying effects of glycosylation on IgG function	1033:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	40	theme	laboratory	1000:1009	arg1	strains					1017:1023	(v) common laboratory mouse strains	989:1023	(v) common laboratory mouse strains	989:1023	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	41	contain	having	580:585	arg2	influences					610:619	the same environmental influences	587:619	the same environmental influences	587:619	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	41	contain	having	580:585	arg1	mice					575:578	all mice	571:578	all mice having the same environmental influences	571:619	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	0	42	theme	immunoglobulin	44:57	arg1	glycome					61:67	the murine immunoglobulin G glycome	33:67	the murine immunoglobulin G glycome	33:67	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	3	43	dep	higher	524:529	arg1	iii					705:707	iii	705:707	iii	705:707	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	44	theme	optimal	1033:1039	arg1	models					1048:1053	optimal animal models	1033:1053	optimal animal models for studying effects of glycosylation on IgG function	1033:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	44	theme	optimal	1033:1039	arg1	strains					1017:1023	(v) common laboratory mouse strains	989:1023	(v) common laboratory mouse strains	989:1023	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	45	theme	common	993:998	arg1	models					1048:1053	optimal animal models	1033:1053	optimal animal models for studying effects of glycosylation on IgG function	1033:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	45	theme	common	993:998	arg1	strains					1017:1023	(v) common laboratory mouse strains	989:1023	(v) common laboratory mouse strains	989:1023	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	46	theme	most	878:881	arg1	studies					892:898	most previous studies	878:898	most previous studies	878:898	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	0	47	theme	murine	37:42	arg1	glycome					61:67	the murine immunoglobulin G glycome	33:67	the murine immunoglobulin G glycome	33:67	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	3	48	theme	murine	678:683	arg1	glycosylation					689:701	murine IgG glycosylation	678:701	murine IgG glycosylation	678:701	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	49	theme	animal	1041:1046	arg1	models					1048:1053	optimal animal models	1033:1053	optimal animal models for studying effects of glycosylation on IgG function	1033:1107	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	49	theme	animal	1041:1046	arg1	strains					1017:1023	(v) common laboratory mouse strains	989:1023	(v) common laboratory mouse strains	989:1023	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	50	theme	important	452:460	arg1	observations					462:473	several important observations	444:473	several important observations	444:473	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	51	theme	glycome	774:780	arg1	variation					782:790	glycome variation	774:790	glycome variation	774:790	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	52	theme	IgG	685:687	arg1	glycosylation					689:701	murine IgG glycosylation	678:701	murine IgG glycosylation	678:701	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	53	theme	strains	427:433	arg1	composition					406:416	the IgG glycome composition	390:416	the IgG glycome composition of 95 CC strains	390:433	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	54	dep	made	439:442	arg1	higher					524:529	higher	524:529	higher	524:529	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	55	theme	same	591:594	arg1	influences					610:619	the same environmental influences	587:619	the same environmental influences	587:619	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	2	56	theme	genetic	295:301	arg1	resource					303:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource	266:310	The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse.					
29632412	3	57	dep	found	650:654	arg1	ii					623:624	ii	623:624	ii	623:624	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	0	58	theme	glycome	61:67	arg1	Profiling					0:8	Profiling	0:8	Profiling	0:8	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	0	58	theme	glycome	61:67	arg1	control					22:28	genetic control	14:28	genetic control	14:28	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	3	59	theme	environmental	596:608	arg1	influences					610:619	the same environmental influences	587:619	the same environmental influences	587:619	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	60	theme	individual	544:553	arg1	humans					555:560	individual humans	544:560	individual humans	544:560	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	61	theme	95	421:422	arg1	CC					424:425	CC	424:425	CC	424:425	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	62	dep	variation	488:496	arg1	i					477:477	i	477:477	i	477:477	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	3	63	theme	glycome	398:404	arg1	composition					406:416	the IgG glycome composition	390:416	the IgG glycome composition of 95 CC strains	390:433	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	0	64	theme	G	59:59	arg1	glycome					61:67	the murine immunoglobulin G glycome	33:67	the murine immunoglobulin G glycome	33:67	Profiling and genetic control of the murine immunoglobulin G glycome.					
29632412	3	65	theme	previous	883:890	arg1	studies					892:898	most previous studies	878:898	most previous studies	878:898	By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.					
29632412	1	66	theme	inter-individual	215:230	arg1	variability					232:242	its inter-individual variability	211:242	its inter-individual variability	211:242	Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined.					
29441788	6	0	theme	hypo/nonglycosylated	717:736	arg1	rhAFM					738:742	hypo/nonglycosylated rhAFM	717:742	hypo/nonglycosylated rhAFM	717:742	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	6	1	theme	culture	768:774	arg1	supernatants					776:787	cell culture supernatants	763:787	cell culture supernatants	763:787	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	9	2	theme	forms	1450:1454	arg1	expression					1399:1408	sufficient expression	1388:1408	sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells	1388:1488	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	3	3	theme	afamin	347:352	arg1	structure					334:342	the structure	330:342	the structure of afamin	330:352	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	7	4	theme	Glycan	858:863	arg1	analysis					865:872	Glycan analysis	858:872	Glycan analysis of purified proteins	858:893	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	7	5	theme	glycosylation	957:969	arg1	macro-heterogeneity					934:952	macro-heterogeneity	934:952	macro-heterogeneity	934:952	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	7	5	theme	glycosylation	957:969	arg1	micro-					923:928	micro-	923:928	micro-	923:928	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	6	6	gly	hypo/nonglycosylated	717:736	arg1	rhAFM					738:742	hypo/nonglycosylated rhAFM	717:742	hypo/nonglycosylated rhAFM	717:742	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	8	7	theme	Fab	1249:1251	arg1	fragment					1253:1260	a Fab fragment	1247:1260	a Fab fragment of the monoclonal anti-afamin antibody N14	1247:1303	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	4	8	theme	CHO	543:545	arg1	cells					570:574	CHO, CHO-Lec1, and HEK293T cells	543:574	CHO, CHO-Lec1, and HEK293T cells	543:574	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	5	9	theme	afamin	695:700	arg1	afamin					695:700	afamin	695:700	afamin	695:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	5	9	theme	afamin	695:700	arg1	sites					686:690	all N-glycosylation sites	666:690	all N-glycosylation sites of afamin	666:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	7	10	theme	plasma	1049:1054	arg1	afamin					1056:1061	native plasma afamin	1042:1061	native plasma afamin	1042:1061	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	3	11	theme	expressed	386:394	arg1	rhAFM					410:414	rhAFM	410:414	rhAFM	410:414	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	3	11	theme	expressed	386:394	arg1	afamin					402:407	recombinantly expressed human afamin	372:407	recombinantly expressed human afamin (rhAFM) with different glycosylation patterns	372:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	4	12	theme	hypoglycosylated	502:517	arg1	forms					519:523	various hypoglycosylated forms	494:523	various hypoglycosylated forms	494:523	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	9	13	theme	CHO	1480:1482	arg1	cells					1484:1488	HEK293T and CHO cells	1468:1488	cells	1484:1488	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	5	14	theme	point-mutated	637:649	arg1	cDNA					651:654	point-mutated cDNA	637:654	point-mutated cDNA	637:654	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	6	15	theme	cell	763:766	arg1	supernatants					776:787	cell culture supernatants	763:787	cell culture supernatants	763:787	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	8	16	theme	antibody	1292:1299	arg1	fragment					1253:1260	a Fab fragment	1247:1260	a Fab fragment of the monoclonal anti-afamin antibody N14	1247:1303	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	4	17	theme	various	494:500	arg1	forms					519:523	various hypoglycosylated forms	494:523	various hypoglycosylated forms	494:523	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	5	18	theme	cDNA	651:654	arg1	transfection					621:632	transfection	621:632	transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin	621:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	8	19	theme	anti-afamin	1280:1290	arg1	antibody					1292:1299	the monoclonal anti-afamin antibody N14	1265:1303	the monoclonal anti-afamin antibody N14	1265:1303	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	9	20	theme	rhAFM	1459:1463	arg1	forms					1450:1454	various partially or nonglycosylated forms	1413:1454	various partially or nonglycosylated forms of rhAFM	1413:1463	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	3	21	theme	different	422:430	arg1	patterns					446:453	different glycosylation patterns	422:453	different glycosylation patterns	422:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	8	22	theme	artificial	1103:1112	arg1	chaperones					1114:1123	artificial chaperones	1103:1123	artificial chaperones	1103:1123	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	8	22	theme	artificial	1103:1112	arg1	chance					1195:1200	the chance	1191:1200	the chance for successful crystallization	1191:1231	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	1	23	with	glycoprotein	96:107	arg1	sites					145:149	five predicted N-glycosylation sites	114:149	five predicted N-glycosylation sites	114:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	7	24	theme	native	1042:1047	arg1	afamin					1056:1061	native plasma afamin	1042:1061	native plasma afamin	1042:1061	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	7	25	gly	macro-heterogeneity	934:952	arg1	glycosylation					957:969	glycosylation	957:969	glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin	957:1061	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	9	26	theme	HEK293T	1468:1474	arg1	cells					1484:1488	HEK293T and CHO cells	1468:1488	cells	1484:1488	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	5	27	theme	N-glycosylation	670:684	arg1	afamin					695:700	afamin	695:700	afamin	695:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	5	27	theme	N-glycosylation	670:684	arg1	sites					686:690	all N-glycosylation sites	666:690	all N-glycosylation sites of afamin	666:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	6	28	theme	exclusion	832:840	arg1	chromatography					842:855	immobilized metal ion affinity and size exclusion chromatography	792:855	chromatography	842:855	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	3	29	with	afamin	402:407	arg1	patterns					446:453	different glycosylation patterns	422:453	different glycosylation patterns	422:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	3	30	theme	human	396:400	arg1	rhAFM					410:414	rhAFM	410:414	rhAFM	410:414	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	3	30	theme	human	396:400	arg1	afamin					402:407	recombinantly expressed human afamin	372:407	recombinantly expressed human afamin (rhAFM) with different glycosylation patterns	372:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	7	31	theme	purified	877:884	arg1	proteins					886:893	purified proteins	877:893	purified proteins	877:893	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	6	32	theme	size	827:830	arg1	chromatography					842:855	immobilized metal ion affinity and size exclusion chromatography	792:855	chromatography	842:855	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	1	33	theme	87	89:90	arg1	kDa					92:94	kDa	92:94	kDa	92:94	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	4	34	gly	hypoglycosylated	502:517	arg1	forms					519:523	various hypoglycosylated forms	494:523	various hypoglycosylated forms	494:523	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	3	35	theme	afamin	402:407	arg1	forms					363:367	various forms	355:367	various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns	355:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	5	36	gly	nonglycosylated	583:597	arg1	rhAFM					599:603	Fully nonglycosylated rhAFM	577:603	Fully nonglycosylated rhAFM	577:603	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	1	37	gly	glycoprotein	96:107	arg1	glycoprotein					96:107	an 87 kDa glycoprotein	86:107	an 87 kDa glycoprotein with five predicted N-glycosylation sites	86:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	1	37	gly	glycoprotein	96:107	arg1	Afamin					76:81	Afamin	76:81	Afamin	76:81	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	4	38	theme	Wild-type	474:482	arg1	rhAFM					484:488	Wild-type rhAFM	474:488	Wild-type rhAFM	474:488	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	1	39	theme	kDa	92:94	arg1	glycoprotein					96:107	an 87 kDa glycoprotein	86:107	an 87 kDa glycoprotein with five predicted N-glycosylation sites	86:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	1	39	theme	kDa	92:94	arg1	Afamin					76:81	Afamin	76:81	Afamin	76:81	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	7	40	theme	proteins	886:893	arg1	analysis					865:872	Glycan analysis	858:872	Glycan analysis of purified proteins	858:893	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	0	41	theme	Biochemical	30:40	arg1	Characterization					42:57	Biochemical Characterization	30:57	Biochemical Characterization of Human Afamin	30:73	Expression, Purification, and Biochemical Characterization of Human Afamin.					
29441788	8	42	theme	successful	1206:1215	arg1	crystallization					1217:1231	successful crystallization	1206:1231	successful crystallization	1206:1231	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	5	43	gly	N-glycosylation	670:684	arg2	afamin					695:700	afamin	695:700	afamin	695:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	5	43	gly	N-glycosylation	670:684	arg1	afamin					695:700	afamin	695:700	afamin	695:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	5	43	gly	N-glycosylation	670:684	arg2	sites					686:690	all N-glycosylation sites	666:690	all N-glycosylation sites of afamin	666:700	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	3	44	theme	glycosylation	432:444	arg1	patterns					446:453	different glycosylation patterns	422:453	different glycosylation patterns	422:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	2	45	theme	glycan	161:166	arg1	abundance					168:176	Afamin's glycan abundance	152:176	Afamin's glycan abundance	152:176	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	2	46	theme	great	246:250	arg1	challenges					252:261	great challenges	246:261	great challenges for molecular structure determination	246:299	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	8	47	theme	stoichiometric	1338:1351	arg1	complex					1353:1359	a stoichiometric complex	1336:1359	a stoichiometric complex	1336:1359	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	4	48	theme	CHO-Lec1	548:555	arg1	cells					570:574	CHO, CHO-Lec1, and HEK293T cells	543:574	CHO, CHO-Lec1, and HEK293T cells	543:574	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	8	49	theme	antibody	1072:1079	arg1	fragments					1081:1089	antibody fragments	1072:1089	antibody fragments	1072:1089	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	0	50	theme	Human	62:66	arg1	Afamin					68:73	Human Afamin	62:73	Human Afamin	62:73	Expression, Purification, and Biochemical Characterization of Human Afamin.					
29441788	7	51	from	differences	908:918	arg1	macro-heterogeneity					934:952	macro-heterogeneity	934:952	macro-heterogeneity	934:952	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	7	51	from	differences	908:918	arg1	micro-					923:928	micro-	923:928	micro-	923:928	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	4	52	theme	HEK293T	562:568	arg1	cells					570:574	CHO, CHO-Lec1, and HEK293T cells	543:574	CHO, CHO-Lec1, and HEK293T cells	543:574	Wild-type rhAFM and various hypoglycosylated forms were expressed in CHO, CHO-Lec1, and HEK293T cells.					
29441788	1	53	gly	N-glycosylation	129:143	arg2	sites					145:149	five predicted N-glycosylation sites	114:149	five predicted N-glycosylation sites	114:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	1	53	gly	N-glycosylation	129:143	arg2	five					114:117	five	114:117	five	114:117	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	2	54	theme	chemical	212:219	arg1	inhomogeneity					221:233	conformational and chemical inhomogeneity	193:233	conformational and chemical inhomogeneity presenting great challenges for molecular structure determination	193:299	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	8	55	theme	proteins	1157:1164	arg1	structure					1144:1152	the structure	1140:1152	the structure of proteins	1140:1164	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	6	56	theme	ion	810:812	arg1	affinity					814:821	immobilized metal ion affinity and size exclusion chromatography	792:855	affinity	814:821	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	9	57	theme	Subsequent	1362:1371	arg1	results					1373:1379	Subsequent results	1362:1379	Subsequent results	1362:1379	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	9	58	theme	sufficient	1388:1397	arg1	expression					1399:1408	sufficient expression	1388:1408	sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells	1388:1488	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	5	59	theme	nonglycosylated	583:597	arg1	rhAFM					599:603	Fully nonglycosylated rhAFM	577:603	Fully nonglycosylated rhAFM	577:603	Fully nonglycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin.					
29441788	7	60	theme	wild-type	1021:1029	arg1	rhAFM					1031:1035	wild-type rhAFM	1021:1035	wild-type rhAFM	1021:1035	Glycan analysis of purified proteins demonstrated differences in micro- and macro-heterogeneity of glycosylation enabling the comparison between hypoglycosylated, wild-type rhAFM, and native plasma afamin.					
29441788	8	61	theme	monoclonal	1269:1278	arg1	antibody					1292:1299	the monoclonal anti-afamin antibody N14	1265:1303	the monoclonal anti-afamin antibody N14	1265:1303	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29441788	6	62	theme	metal	804:808	arg1	affinity					814:821	immobilized metal ion affinity and size exclusion chromatography	792:855	affinity	814:821	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	2	63	theme	conformational	193:206	arg1	inhomogeneity					221:233	conformational and chemical inhomogeneity	193:233	conformational and chemical inhomogeneity presenting great challenges for molecular structure determination	193:299	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	2	64	theme	structure	277:285	arg1	determination					287:299	molecular structure determination	267:299	molecular structure determination	267:299	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	6	65	theme	immobilized	792:802	arg1	affinity					814:821	immobilized metal ion affinity and size exclusion chromatography	792:855	affinity	814:821	Wild-type and hypo/nonglycosylated rhAFM were purified from cell culture supernatants by immobilized metal ion affinity and size exclusion chromatography.					
29441788	1	66	theme	predicted	119:127	arg1	sites					145:149	five predicted N-glycosylation sites	114:149	five predicted N-glycosylation sites	114:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	9	67	from	expression	1399:1408	arg1	cells					1484:1488	HEK293T and CHO cells	1468:1488	cells	1484:1488	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	0	68	theme	Afamin	68:73	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Expression, Purification, and Biochemical Characterization of Human Afamin.					
29441788	0	68	theme	Afamin	68:73	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression, Purification, and Biochemical Characterization of Human Afamin.					
29441788	0	68	theme	Afamin	68:73	arg1	Characterization					42:57	Biochemical Characterization	30:57	Biochemical Characterization of Human Afamin	30:73	Expression, Purification, and Biochemical Characterization of Human Afamin.					
29441788	2	69	theme	molecular	267:275	arg1	determination					287:299	molecular structure determination	267:299	molecular structure determination	267:299	Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination.					
29441788	1	70	theme	N-glycosylation	129:143	arg1	sites					145:149	five predicted N-glycosylation sites	114:149	five predicted N-glycosylation sites	114:149	Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites.					
29441788	3	71	theme	various	355:361	arg1	forms					363:367	various forms	355:367	various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns	355:453	For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created.					
29441788	9	72	theme	various	1413:1419	arg1	forms					1450:1454	various partially or nonglycosylated forms	1413:1454	various partially or nonglycosylated forms of rhAFM	1413:1463	Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion.					
29441788	8	73	theme	human	1310:1314	arg1	afamin					1316:1321	human afamin	1310:1321	human afamin	1310:1321	Because antibody fragments can work as artificial chaperones by stabilizing the structure of proteins and consequently enhance the chance for successful crystallization, we incubated a Fab fragment of the monoclonal anti-afamin antibody N14 with human afamin and obtained a stoichiometric complex.					
29408873	8	0	theme	mutant	1550:1555	arg1	proteins					1560:1567	the mutant Fc proteins	1546:1567	the mutant Fc proteins	1546:1567	Changes in NMR spectra of the mutant Fc proteins mirrored these changes in affinity.					
29408873	8	1	theme	proteins	1560:1567	arg1	spectra					1535:1541	NMR spectra	1531:1541	NMR spectra of the mutant Fc proteins	1531:1567	Changes in NMR spectra of the mutant Fc proteins mirrored these changes in affinity.					
29408873	3	2	dep	Å	679:679	arg1	to					671:672	to	671:672	to	671:672	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	5	3	from	strands	1133:1139	arg1	glycoform					1168:1176	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	6	4	theme	receptor	1362:1369	arg1	affinity					1379:1386	receptor binding affinity	1362:1386	receptor binding affinity	1362:1386	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	3	5	theme	N	726:726	arg1	-glycan					728:734	GlcNAc asparagine-linked (N)-glycan	700:734	a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	685:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	2	6	theme	mouse	550:554	arg1	IgG2b					556:560	mouse IgG2b	550:560	mouse IgG2b	550:560	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	4	7	theme	complex-type	1017:1028	arg1	glycoform					1030:1038	the complex-type glycoform	1013:1038	the complex-type glycoform	1013:1038	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	3	8	theme	asparagine-linked	707:723	arg1	-glycan					728:734	GlcNAc asparagine-linked (N)-glycan	700:734	a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	685:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	7	9	from	differences	1409:1419	arg1	loops					1440:1444	FG loops	1437:1444	FG loops	1437:1444	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	7	9	from	differences	1409:1419	arg1	DE					1430:1431	mIgG2 DE	1424:1431	mIgG2 DE	1424:1431	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	4	10	theme	different	860:868	arg1	interactions					881:892	different non-bonded interactions	860:892	different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b	860:957	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	6	11	theme	N-linked	1267:1274	arg1	residues					1286:1293	the N-linked (1)GlcNAc residues	1263:1293	the N-linked (1)GlcNAc residues	1263:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	1	12	theme	monoclonal	407:416	arg1	antibody					418:425	therapeutic monoclonal antibody	395:425	therapeutic monoclonal antibody	395:425	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	3	13	theme	Fc	599:600	arg1	Models					582:587	Models	582:587	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	582:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	1	14	theme	alternative	212:222	arg1	motifs					235:240	alternative structural motifs	212:240	alternative structural motifs	212:240	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	5	15	theme	longer	1121:1126	arg1	strands					1133:1139	longer beta strands	1121:1139	longer beta strands in the truncated mIgG2c Fc glycoform	1121:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	3	16	theme	mouse	800:804	arg1	receptor					811:818	mouse Fc γ receptor IV	800:821	mouse Fc γ receptor IV (mFcγRIV) binding	800:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	16	theme	mouse	800:804	arg1	mFcγRIV					824:830	mFcγRIV	824:830	mFcγRIV	824:830	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	6	17	theme	NMR	1243:1245	arg1	spectroscopy					1247:1258	Solution NMR spectroscopy	1234:1258	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues	1234:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	1	18	theme	non-human	121:129	arg1	antibodies					131:140	non-human antibodies	121:140	non-human antibodies	121:140	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	3	19	theme	related	789:795	arg1	regions					781:787	key regions	777:787	key regions related to mouse Fc γ receptor IV (mFcγRIV) binding	777:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	20	theme	2.04	749:752	arg1	Å					754:754	2.04 Å	749:754	2.04 Å	749:754	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	5	21	theme	structural	1051:1060	arg1	elements					1062:1069	Secondary structural elements	1041:1069	Secondary structural elements surrounding the Asn297 site of glycosylation	1041:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	9	22	theme	structural	1628:1637	arg1	differences					1654:1664	structural and functional differences	1628:1664	structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison	1628:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	7	23	theme	mIgG2b	1512:1517	arg1	affinity					1500:1507	affinity	1500:1507	affinity of mIgG2b	1500:1517	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	2	24	theme	crystallizable	507:520	arg1	fragment					522:529	the mouse IgG2c crystallizable fragment	491:529	the mouse IgG2c crystallizable fragment (Fc)	491:534	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	2	24	theme	crystallizable	507:520	arg1	Fc					532:533	Fc	532:533	Fc	532:533	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	9	25	theme	conserved	1676:1684	arg1	molecules					1686:1694	highly conserved molecules	1669:1694	highly conserved molecules that were not predicted from primary sequence comparison	1669:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	3	26	theme	key	777:779	arg1	regions					781:787	key regions	777:787	key regions related to mouse Fc γ receptor IV (mFcγRIV) binding	777:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	0	27	theme	mouse	76:80	arg1	IgG2b					82:86	mouse IgG2b	76:86	mouse IgG2b	76:86	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	3	28	theme	γ	809:809	arg1	receptor					811:818	mouse Fc γ receptor IV	800:821	mouse Fc γ receptor IV (mFcγRIV) binding	800:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	28	theme	γ	809:809	arg1	mFcγRIV					824:830	mFcγRIV	824:830	mFcγRIV	824:830	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	29	dep	-glycan	728:734	arg1	1					698:698	1	698:698	1	698:698	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	9	30	theme	primary	1725:1731	arg1	comparison					1742:1751	primary sequence comparison	1725:1751	primary sequence comparison	1725:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	5	31	theme	mIgG2c	1158:1163	arg1	glycoform					1168:1176	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	2	32	theme	receptor-binding	460:475	arg1	properties					477:486	receptor-binding properties	460:486	receptor-binding properties	460:486	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	0	33	theme	Mouse	0:4	arg1	residues					20:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	3	34	from	differences	762:772	arg1	regions					781:787	key regions	777:787	key regions related to mouse Fc γ receptor IV (mFcγRIV) binding	777:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	0	35	theme	Fc	12:13	arg1	residues					20:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	5	36	theme	beta	1128:1131	arg1	strands					1133:1139	longer beta strands	1121:1139	longer beta strands in the truncated mIgG2c Fc glycoform	1121:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	7	37	theme	mIgG2c	1468:1473	arg1	affinity					1456:1463	affinity	1456:1463	affinity of mIgG2c for FcγRIV	1456:1484	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	2	38	theme	mouse	495:499	arg1	fragment					522:529	the mouse IgG2c crystallizable fragment	491:529	the mouse IgG2c crystallizable fragment (Fc)	491:534	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	2	38	theme	mouse	495:499	arg1	Fc					532:533	Fc	532:533	Fc	532:533	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	1	39	theme	protein	309:315	arg1	understanding					292:304	a basic understanding	284:304	a basic understanding of protein and immune system evolution	284:343	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	5	40	theme	Asn297	1087:1092	arg1	glycosylation					1102:1114	glycosylation	1102:1114	glycosylation	1102:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	5	40	theme	Asn297	1087:1092	arg1	site					1094:1097	the Asn297 site	1083:1097	the Asn297 site of glycosylation	1083:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	0	41	theme	greater	37:43	arg1	affinity					62:69	greater receptor-binding affinity	37:69	greater receptor-binding affinity than mouse IgG2b or human IgG1	37:100	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	1	42	theme	immune	321:326	arg1	system					328:333	immune system	321:333	immune system	321:333	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	5	43	theme	Fc	1165:1166	arg1	glycoform					1168:1176	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	0	44	theme	receptor-binding	45:60	arg1	affinity					62:69	greater receptor-binding affinity	37:69	greater receptor-binding affinity than mouse IgG2b or human IgG1	37:100	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	7	45	theme	mIgG2	1424:1428	arg1	DE					1430:1431	mIgG2 DE	1424:1431	mIgG2 DE	1424:1431	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	3	46	theme	truncated	687:695	arg1	-glycan					728:734	GlcNAc asparagine-linked (N)-glycan	700:734	a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	685:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	6	47	theme	Solution	1234:1241	arg1	spectroscopy					1247:1258	Solution NMR spectroscopy	1234:1258	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues	1234:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	1	48	theme	properties	255:264	arg1	identification					194:207	the identification	190:207	the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs	190:382	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	5	49	theme	truncated	1148:1156	arg1	glycoform					1168:1176	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	8	50	theme	Fc	1557:1558	arg1	proteins					1560:1567	the mutant Fc proteins	1546:1567	the mutant Fc proteins	1546:1567	Changes in NMR spectra of the mutant Fc proteins mirrored these changes in affinity.					
29408873	3	51	theme	GlcNAc	700:705	arg1	-glycan					728:734	GlcNAc asparagine-linked (N)-glycan	700:734	a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	685:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	8	52	from	Changes	1520:1526	arg1	spectra					1535:1541	NMR spectra	1531:1541	NMR spectra of the mutant Fc proteins	1531:1567	Changes in NMR spectra of the mutant Fc proteins mirrored these changes in affinity.					
29408873	2	53	dep	IgG2b	556:560	arg1	Fcs					577:579	Fcs	577:579	Fcs	577:579	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	4	54	theme	Mouse	842:846	arg1	IgG2c					848:852	Mouse IgG2c	842:852	Mouse IgG2c	842:852	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	1	55	theme	therapeutic	395:405	arg1	antibody					418:425	therapeutic monoclonal antibody	395:425	therapeutic monoclonal antibody	395:425	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	3	56	theme	mIgG2c	592:597	arg1	Fc					599:600	mIgG2c Fc	592:600	mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	592:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	9	57	theme	functional	1643:1652	arg1	differences					1654:1664	structural and functional differences	1628:1664	structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison	1628:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	1	58	theme	system	328:333	arg1	understanding					292:304	a basic understanding	284:304	a basic understanding of protein and immune system evolution	284:343	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	5	59	theme	complex-type	1211:1222	arg1	N-glycan					1224:1231	the complex-type N-glycan	1207:1231	the complex-type N-glycan	1207:1231	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	3	60	theme	x-ray	616:620	arg1	crystallography					622:636	x-ray crystallography	616:636	x-ray crystallography	616:636	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	4	61	from	affinity	1001:1008	arg1	glycoform					1030:1038	the complex-type glycoform	1013:1038	the complex-type glycoform	1013:1038	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	3	62	theme	Fc	806:807	arg1	receptor					811:818	mouse Fc γ receptor IV	800:821	mouse Fc γ receptor IV (mFcγRIV) binding	800:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	62	theme	Fc	806:807	arg1	mFcγRIV					824:830	mFcγRIV	824:830	mFcγRIV	824:830	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	2	63	theme	human	566:570	arg1	IgG1					572:575	human IgG1	566:575	human IgG1	566:575	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	3	64	link	asparagine-linked	707:723	arg1	-glycan					728:734	GlcNAc asparagine-linked (N)-glycan	700:734	a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å)	685:755	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	1	65	theme	antibodies	131:140	arg1	structures					107:116	The structures	103:116	The structures of non-human antibodies	103:140	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	1	65	theme	antibodies	131:140	arg1	unstudied					154:162	unstudied	154:162	unstudied	154:162	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	4	66	theme	greater	993:999	arg1	affinity					1001:1008	4.7-fold greater affinity	984:1008	4.7-fold greater affinity in the complex-type glycoform	984:1038	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	9	67	from	differences	1654:1664	arg1	molecules					1686:1694	highly conserved molecules	1669:1694	highly conserved molecules that were not predicted from primary sequence comparison	1669:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	2	68	theme	fragment	522:529	arg1	structure					446:454	structure	446:454	structure	446:454	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	2	68	theme	fragment	522:529	arg1	properties					477:486	receptor-binding properties	460:486	receptor-binding properties	460:486	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	5	69	gly	glycosylation	1102:1114	arg2	glycosylation					1102:1114	glycosylation	1102:1114	glycosylation	1102:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	5	69	gly	glycosylation	1102:1114	arg2	site					1094:1097	the Asn297 site	1083:1097	the Asn297 site of glycosylation	1083:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	1	70	theme	structural	224:233	arg1	motifs					235:240	alternative structural motifs	212:240	alternative structural motifs	212:240	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	2	71	theme	IgG2c	501:505	arg1	fragment					522:529	the mouse IgG2c crystallizable fragment	491:529	the mouse IgG2c crystallizable fragment (Fc)	491:534	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	2	71	theme	IgG2c	501:505	arg1	Fc					532:533	Fc	532:533	Fc	532:533	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	5	72	gly	glycoform	1168:1176	arg1	mIgG2c					1158:1163	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	5	72	gly	glycoform	1168:1176	arg1	Fc					1165:1166	the truncated mIgG2c Fc glycoform	1144:1176	the truncated mIgG2c Fc glycoform	1144:1176	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	0	73	theme	human	91:95	arg1	IgG1					97:100	human IgG1	91:100	human IgG1	91:100	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	3	74	theme	receptor	811:818	arg1	binding					833:839	mouse Fc γ receptor IV (mFcγRIV) binding	800:839	mouse Fc γ receptor IV (mFcγRIV) binding	800:839	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	4	75	theme	non-bonded	870:879	arg1	interactions					881:892	different non-bonded interactions	860:892	different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b	860:957	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	6	76	theme	residues	1286:1293	arg1	spectroscopy					1247:1258	Solution NMR spectroscopy	1234:1258	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues	1234:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	1	77	theme	unexplored	366:375	arg1	motifs					377:382	unexplored motifs	366:382	unexplored motifs	366:382	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	2	78	dep	structure	446:454	arg1	the					442:444	the	442:444	the	442:444	Here we probe the structure and receptor-binding properties of the mouse IgG2c crystallizable fragment (Fc) to compare to mouse IgG2b and human IgG1 Fcs.					
29408873	9	79	theme	sequence	1733:1740	arg1	comparison					1742:1751	primary sequence comparison	1725:1751	primary sequence comparison	1725:1751	Our studies identified structural and functional differences in highly conserved molecules that were not predicted from primary sequence comparison.					
29408873	1	80	dep	protein	309:315	arg1	evolution					335:343	evolution	335:343	evolution	335:343	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	0	81	theme	IgG2c	6:10	arg1	residues					20:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	5	82	theme	Secondary	1041:1049	arg1	elements					1062:1069	Secondary structural elements	1041:1069	Secondary structural elements surrounding the Asn297 site of glycosylation	1041:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	4	83	theme	highly-conserved	935:950	arg1	mIgG2b					952:957	the highly-conserved mIgG2b	931:957	the highly-conserved mIgG2b	931:957	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	0	84	theme	loop	15:18	arg1	residues					20:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues	0:27	Mouse IgG2c Fc loop residues promote greater receptor-binding affinity than mouse IgG2b or human IgG1.					
29408873	1	85	theme	basic	286:290	arg1	understanding					292:304	a basic understanding	284:304	a basic understanding of protein and immune system evolution	284:343	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	3	86	theme	complex-type	645:656	arg1	biantennary					658:668	a complex-type biantennary	643:668	a complex-type biantennary (to 2.05 Å)	643:680	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	3	86	theme	complex-type	645:656	arg1	Å					679:679	to 2.05 Å	671:679	to 2.05 Å	671:679	Models of mIgG2c Fc determined by x-ray crystallography with a complex-type biantennary (to 2.05 Å) or a truncated (1)GlcNAc asparagine-linked (N)-glycan attached (to 2.04 Å) show differences in key regions related to mouse Fc γ receptor IV (mFcγRIV) binding.					
29408873	4	87	theme	FG	917:918	arg1	loops					920:924	FG loops	917:924	FG loops	917:924	Mouse IgG2c forms different non-bonded interactions between the BC, DE and FG loops than the highly-conserved mIgG2b and binds to FcγRIV with 4.7-fold greater affinity in the complex-type glycoform.					
29408873	6	88	theme	GlcNAc	1279:1284	arg1	residues					1286:1293	the N-linked (1)GlcNAc residues	1263:1293	the N-linked (1)GlcNAc residues	1263:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	6	89	theme	hIgG1	1335:1339	arg1	Fc					1341:1342	hIgG1 Fc	1335:1342	hIgG1 Fc	1335:1342	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	5	90	theme	glycosylation	1102:1114	arg1	glycosylation					1102:1114	glycosylation	1102:1114	glycosylation	1102:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	5	90	theme	glycosylation	1102:1114	arg1	site					1094:1097	the Asn297 site	1083:1097	the Asn297 site of glycosylation	1083:1114	Secondary structural elements surrounding the Asn297 site of glycosylation form longer beta strands in the truncated mIgG2c Fc glycoform when compared to mIgG2c with the complex-type N-glycan.					
29408873	6	91	link	N-linked	1267:1274	arg1	residues					1286:1293	the N-linked (1)GlcNAc residues	1263:1293	the N-linked (1)GlcNAc residues	1263:1293	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	8	92	theme	NMR	1531:1533	arg1	spectra					1535:1541	NMR spectra	1531:1541	NMR spectra of the mutant Fc proteins	1531:1567	Changes in NMR spectra of the mutant Fc proteins mirrored these changes in affinity.					
29408873	1	93	theme	motifs	235:240	arg1	identification					194:207	the identification	190:207	the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs	190:382	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29408873	7	94	theme	FG	1437:1438	arg1	loops					1440:1444	FG loops	1437:1444	FG loops	1437:1444	Mutations targeting differences in mIgG2 DE and FG loops decreased affinity of mIgG2c for FcγRIV and increased affinity of mIgG2b.					
29408873	6	95	theme	binding	1371:1377	arg1	affinity					1379:1386	receptor binding affinity	1362:1386	receptor binding affinity	1362:1386	Solution NMR spectroscopy of the N-linked (1)GlcNAc residues show differences between mIgG2b, 2c and hIgG1 Fc that correlate to receptor binding affinity.					
29408873	1	96	theme	physical	246:253	arg1	properties					255:264	physical properties	246:264	physical properties	246:264	The structures of non-human antibodies are largely unstudied despite the potential for the identification of alternative structural motifs and physical properties that will benefit a basic understanding of protein and immune system evolution as well as highlight unexplored motifs to improve therapeutic monoclonal antibody.					
29596458	8	0	theme	positive	1056:1063	arg1	culture					1071:1077	confirmed positive blood culture	1046:1077	confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1046:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	11	1	theme	structures-discriminant	1562:1584	arg1	S-Plot					1596:1601	S-Plot	1596:1601	S-Plot	1596:1601	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	11	1	theme	structures-discriminant	1562:1584	arg1	analysis					1586:1593	latent structures-discriminant analysis	1555:1593	latent structures-discriminant analysis (S-Plot)	1555:1602	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	6	2	theme	potential	715:723	arg1	biomarkers					704:713	biomarkers	704:713	biomarkers potential of molecular profiling of plasma	704:756	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	6	3	theme	bloodstream	819:829	arg1	infections					831:840	bloodstream infections	819:840	bloodstream infections	819:840	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	12	4	theme	patient	1757:1763	arg1	classes					1765:1771	the two patient classes	1749:1771	the two patient classes	1749:1771	These methods gave us clear separation of the two patient classes.					
29596458	10	5	theme	high	1359:1362	arg1	confidence					1364:1373	high confidence	1359:1373	high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures	1359:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	10	6	gly	N-glycopeptides	1322:1336	arg2	N-glycopeptides					1322:1336	Twenty four N-glycopeptides	1310:1336	Twenty four N-glycopeptides	1310:1336	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	4	7	theme	blood	470:474	arg1	sampling					476:483	blood sampling	470:483	blood sampling	470:483	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	2	8	theme	antibiotic	276:285	arg1	therapy					287:293	antibiotic therapy	276:293	antibiotic therapy	276:293	Appropriate and timely onset of antibiotic therapy influences the prognosis of these patients.					
29596458	3	9	theme	diagnostic	355:364	arg1	accuracy					366:373	the diagnostic accuracy	351:373	the diagnostic accuracy which is not afforded by current gold standards such as blood culture	351:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	11	10	theme	other	1646:1650	arg1	methods					1664:1670	other statistical methods	1646:1670	other statistical methods	1646:1670	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	0	11	theme	plasma	79:84	arg1	proteins					86:93	various plasma proteins	71:93	various plasma proteins in bloodstream infected patients	71:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	12	12	theme	us	1726:1727	arg1	separation					1735:1744	us clear separation	1726:1744	us clear separation of the two patient classes	1726:1771	These methods gave us clear separation of the two patient classes.					
29596458	1	13	with	mortality	191:199	arg1	rates					206:210	rates	206:210	rates varying from 10-25% and higher	206:241	Bloodstream infections are associated with high morbidity and mortality with rates varying from 10-25% and higher.					
29596458	10	14	dep	together	1375:1382	arg1	with					1384:1387	with	1384:1387	with	1384:1387	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	4	15	theme	key	515:517	arg1	time					460:463	the time	456:463	the time from blood sampling to blood culture results	456:508	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	4	15	theme	key	515:517	arg1	determinant					519:529	a key determinant	513:529	a key determinant of reducing mortality	513:551	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	13	16	theme	culture	1888:1894	arg1	patients					1913:1920	blood culture negative febrile patients	1882:1920	blood culture negative febrile patients	1882:1920	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	0	17	theme	infected	110:117	arg1	patients					119:126	bloodstream infected patients	98:126	bloodstream infected patients	98:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	1	18	with	morbidity	177:185	arg1	rates					206:210	rates	206:210	rates varying from 10-25% and higher	206:241	Bloodstream infections are associated with high morbidity and mortality with rates varying from 10-25% and higher.					
29596458	5	19	theme	other	638:642	arg1	conditions					666:675	other systemic inflammatory conditions	638:675	other systemic inflammatory conditions	638:675	No established biomarkers exist which can differentiate bloodstream infections from other systemic inflammatory conditions.					
29596458	9	20	theme	potential	1197:1205	arg1	N-glycopeptides					1207:1221	Three hundred and sixty eight potential N-glycopeptides	1167:1221	Three hundred and sixty eight potential N-glycopeptides	1167:1221	Three hundred and sixty eight potential N-glycopeptides were quantified by mass spectrometry and 149 were further selected for identification.					
29596458	6	21	theme	plasma	751:756	arg1	profiling					738:746	molecular profiling	728:746	molecular profiling of plasma	728:756	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	8	22	theme	negative	1135:1142	arg1	reports					1158:1164	negative blood culture reports	1135:1164	negative blood culture reports	1135:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	3	23	theme	gold	408:411	arg1	standards					413:421	current gold standards	400:421	current gold standards such as blood culture	400:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	3	23	theme	gold	408:411	arg1	culture					437:443	blood culture	431:443	blood culture	431:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	4	24	theme	culture	494:500	arg1	results					502:508	blood culture results	488:508	blood culture results	488:508	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	13	25	theme	bloodstream	1854:1864	arg1	infections					1866:1875	the bloodstream infections	1850:1875	the bloodstream infections	1850:1875	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	6	26	theme	profiling	738:746	arg1	potential					715:723	potential	715:723	potential	715:723	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	0	27	theme	Quantitative	0:11	arg1	N-glycoproteomics					13:29	Quantitative N-glycoproteomics	0:29	Quantitative N-glycoproteomics	0:29	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	8	28	theme	culture	1150:1156	arg1	reports					1158:1164	negative blood culture reports	1135:1164	negative blood culture reports	1135:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	10	29	theme	site	1442:1445	arg1	elucidation					1389:1399	elucidation	1389:1399	high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures	1359:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	1	30	theme	Bloodstream	129:139	arg1	infections					141:150	Bloodstream infections	129:150	Bloodstream infections	129:150	Bloodstream infections are associated with high morbidity and mortality with rates varying from 10-25% and higher.					
29596458	8	31	theme	quantitative	978:989	arg1	N-glycoproteomics					991:1007	targeted quantitative N-glycoproteomics	969:1007	targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	969:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	3	32	theme	blood	431:435	arg1	culture					437:443	blood culture	431:443	blood culture	431:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	11	33	theme	self-organizing	1608:1622	arg1	maps					1624:1627	self-organizing maps	1608:1627	self-organizing maps clustering among other statistical methods	1608:1670	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	0	34	theme	glycosylation	47:59	arg1	levels					61:66	altered glycosylation levels	39:66	altered glycosylation levels of various plasma proteins in bloodstream infected patients	39:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	8	35	with	patients	1032:1039	arg1	culture					1071:1077	confirmed positive blood culture	1046:1077	confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1046:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	6	36	theme	molecular	788:796	arg1	changes					798:804	the molecular changes	784:804	the molecular changes accompanying bloodstream infections	784:840	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	4	37	theme	mortality	543:551	arg1	time					460:463	the time	456:463	the time from blood sampling to blood culture results	456:508	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	4	37	theme	mortality	543:551	arg1	determinant					519:529	a key determinant	513:529	a key determinant of reducing mortality	513:551	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	9	38	theme	mass	1242:1245	arg1	spectrometry					1247:1258	mass spectrometry	1242:1258	mass spectrometry	1242:1258	Three hundred and sixty eight potential N-glycopeptides were quantified by mass spectrometry and 149 were further selected for identification.					
29596458	8	39	theme	patients	1032:1039	arg1	samples					1021:1027	plasma samples	1014:1027	plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1014:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	7	40	from	changes	923:929	arg1	physiology					934:943	physiology	934:943	physiology	934:943	N-glycosylation is a post-translational modification which is very sensitive to changes in physiology.					
29596458	8	41	theme	confirmed	1046:1054	arg1	culture					1071:1077	confirmed positive blood culture	1046:1077	confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1046:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	13	42	gly	N-glycopeptides	1801:1815	arg2	N-glycopeptides					1801:1815	high-confidence N-glycopeptides	1785:1815	high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia	1785:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	11	43	theme	component	1509:1517	arg1	analysis					1519:1526	Principal component analysis	1499:1526	Principal component analysis	1499:1526	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	10	44	theme	glycan	1480:1485	arg1	structures					1487:1496	proposed glycan structures	1471:1496	proposed glycan structures	1471:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	8	45	theme	blood	1065:1069	arg1	culture					1071:1077	confirmed positive blood culture	1046:1077	confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1046:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	11	46	theme	latent	1555:1560	arg1	S-Plot					1596:1601	S-Plot	1596:1601	S-Plot	1596:1601	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	11	46	theme	latent	1555:1560	arg1	analysis					1586:1593	latent structures-discriminant analysis	1555:1593	latent structures-discriminant analysis (S-Plot)	1555:1602	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	5	47	theme	inflammatory	653:664	arg1	conditions					666:675	other systemic inflammatory conditions	638:675	other systemic inflammatory conditions	638:675	No established biomarkers exist which can differentiate bloodstream infections from other systemic inflammatory conditions.					
29596458	14	48	with	ProteomeXchange	1996:2010	arg1	identifier					2017:2026	identifier PXD009048	2017:2036	identifier PXD009048	2017:2036	Data are available via ProteomeXchange with identifier PXD009048.					
29596458	12	49	theme	classes	1765:1771	arg1	separation					1735:1744	us clear separation	1726:1744	us clear separation of the two patient classes	1726:1771	These methods gave us clear separation of the two patient classes.					
29596458	8	50	theme	matched	1105:1111	arg1	controls					1121:1128	matched febrile controls	1105:1128	matched febrile controls	1105:1128	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	13	51	theme	host	1940:1943	arg1	response					1945:1952	host response	1940:1952	host response during bacteremia	1940:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	4	52	from	sampling	476:483	arg1	time					460:463	the time	456:463	the time from blood sampling to blood culture results	456:508	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	4	52	from	sampling	476:483	arg1	determinant					519:529	a key determinant	513:529	a key determinant of reducing mortality	513:551	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	2	53	theme	patients	329:336	arg1	prognosis					310:318	the prognosis	306:318	the prognosis of these patients	306:336	Appropriate and timely onset of antibiotic therapy influences the prognosis of these patients.					
29596458	13	54	theme	febrile	1905:1911	arg1	patients					1913:1920	blood culture negative febrile patients	1882:1920	blood culture negative febrile patients	1882:1920	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	2	55	theme	timely	260:265	arg1	onset					267:271	Appropriate and timely onset	244:271	Appropriate and timely onset of antibiotic therapy	244:293	Appropriate and timely onset of antibiotic therapy influences the prognosis of these patients.					
29596458	13	56	contain	have	1823:1826	arg1	N-glycopeptides					1801:1815	high-confidence N-glycopeptides	1785:1815	high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia	1785:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	13	56	contain	have	1823:1826	arg2	power					1832:1836	the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia	1828:1970	the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia	1828:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	10	57	gly	N-glycosylation	1426:1440	arg2	site					1442:1445	N-glycosylation site	1426:1445	N-glycosylation site	1426:1445	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	0	58	from	proteins	86:93	arg1	patients					119:126	bloodstream infected patients	98:126	bloodstream infected patients	98:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	2	59	theme	Appropriate	244:254	arg1	onset					267:271	Appropriate and timely onset	244:271	Appropriate and timely onset of antibiotic therapy	244:293	Appropriate and timely onset of antibiotic therapy influences the prognosis of these patients.					
29596458	5	60	theme	established	557:567	arg1	biomarkers					569:578	No established biomarkers	554:578	No established biomarkers	554:578	No established biomarkers exist which can differentiate bloodstream infections from other systemic inflammatory conditions.					
29596458	6	61	from	studies	693:699	arg1	biomarkers					704:713	biomarkers	704:713	biomarkers potential of molecular profiling of plasma	704:756	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	11	62	theme	statistical	1652:1662	arg1	methods					1664:1670	other statistical methods	1646:1670	other statistical methods	1646:1670	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	2	63	theme	therapy	287:293	arg1	onset					267:271	Appropriate and timely onset	244:271	Appropriate and timely onset of antibiotic therapy	244:293	Appropriate and timely onset of antibiotic therapy influences the prognosis of these patients.					
29596458	10	64	theme	glycan	1448:1453	arg1	composition					1455:1465	glycan composition	1448:1465	glycan composition	1448:1465	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	0	65	from	patients	119:126	arg1	levels					61:66	altered glycosylation levels	39:66	altered glycosylation levels of various plasma proteins in bloodstream infected patients	39:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	0	66	theme	proteins	86:93	arg1	levels					61:66	altered glycosylation levels	39:66	altered glycosylation levels of various plasma proteins in bloodstream infected patients	39:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	1	67	theme	high	172:175	arg1	morbidity					177:185	morbidity	177:185	morbidity	177:185	Bloodstream infections are associated with high morbidity and mortality with rates varying from 10-25% and higher.					
29596458	12	68	theme	clear	1729:1733	arg1	separation					1735:1744	us clear separation	1726:1744	us clear separation of the two patient classes	1726:1771	These methods gave us clear separation of the two patient classes.					
29596458	8	69	with	culture	1071:1077	arg1	reports					1158:1164	negative blood culture reports	1135:1164	negative blood culture reports	1135:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	0	70	theme	bloodstream	98:108	arg1	patients					119:126	bloodstream infected patients	98:126	bloodstream infected patients	98:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	8	71	theme	febrile	1113:1119	arg1	controls					1121:1128	matched febrile controls	1105:1128	matched febrile controls	1105:1128	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	13	72	theme	blood	1882:1886	arg1	patients					1913:1920	blood culture negative febrile patients	1882:1920	blood culture negative febrile patients	1882:1920	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	13	73	from	patients	1913:1920	arg1	separate					1841:1848	separate	1841:1848	separate	1841:1848	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	4	74	theme	blood	488:492	arg1	results					502:508	blood culture results	488:508	blood culture results	488:508	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	13	75	theme	negative	1896:1903	arg1	patients					1913:1920	blood culture negative febrile patients	1882:1920	blood culture negative febrile patients	1882:1920	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	10	76	theme	sequence	1416:1423	arg1	elucidation					1389:1399	elucidation	1389:1399	high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures	1359:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	8	77	theme	blood	1144:1148	arg1	reports					1158:1164	negative blood culture reports	1135:1164	negative blood culture reports	1135:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	5	78	theme	systemic	644:651	arg1	conditions					666:675	other systemic inflammatory conditions	638:675	other systemic inflammatory conditions	638:675	No established biomarkers exist which can differentiate bloodstream infections from other systemic inflammatory conditions.					
29596458	8	79	from	samples	1021:1027	arg1	N-glycoproteomics					991:1007	targeted quantitative N-glycoproteomics	969:1007	targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	969:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	0	80	from	levels	61:66	arg1	patients					119:126	bloodstream infected patients	98:126	bloodstream infected patients	98:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	8	81	dep	age	1093:1095	arg1	controls					1121:1128	matched febrile controls	1105:1128	matched febrile controls	1105:1128	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	6	82	theme	molecular	728:736	arg1	profiling					738:746	molecular profiling	728:746	molecular profiling of plasma	728:756	This calls for studies on biomarkers potential of molecular profiling of plasma as it is affected most by the molecular changes accompanying bloodstream infections.					
29596458	0	83	theme	altered	39:45	arg1	levels					61:66	altered glycosylation levels	39:66	altered glycosylation levels of various plasma proteins in bloodstream infected patients	39:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	7	84	theme	post-translational	864:881	arg1	modification					883:894	a post-translational modification	862:894	a post-translational modification which is very sensitive to changes in physiology	862:943	N-glycosylation is a post-translational modification which is very sensitive to changes in physiology.					
29596458	7	84	theme	post-translational	864:881	arg1	sensitive					910:918	sensitive	910:918	sensitive	910:918	N-glycosylation is a post-translational modification which is very sensitive to changes in physiology.					
29596458	7	84	theme	post-translational	864:881	arg1	N-glycosylation					843:857	N-glycosylation	843:857	N-glycosylation	843:857	N-glycosylation is a post-translational modification which is very sensitive to changes in physiology.					
29596458	5	85	theme	bloodstream	610:620	arg1	infections					622:631	bloodstream infections	610:631	bloodstream infections	610:631	No established biomarkers exist which can differentiate bloodstream infections from other systemic inflammatory conditions.					
29596458	3	86	theme	current	400:406	arg1	standards					413:421	current gold standards	400:421	current gold standards such as blood culture	400:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	3	86	theme	current	400:406	arg1	culture					437:443	blood culture	431:443	blood culture	431:443	It requires the diagnostic accuracy which is not afforded by current gold standards such as blood culture.					
29596458	10	87	theme	N-glycosylation	1426:1440	arg1	site					1442:1445	N-glycosylation site	1426:1445	N-glycosylation site	1426:1445	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	10	88	theme	peptide	1408:1414	arg1	sequence					1416:1423	the peptide sequence	1404:1423	the peptide sequence	1404:1423	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	8	89	theme	targeted	969:976	arg1	N-glycoproteomics					991:1007	targeted quantitative N-glycoproteomics	969:1007	targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	969:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	4	90	theme	reducing	534:541	arg1	mortality					543:551	reducing mortality	534:551	reducing mortality	534:551	Moreover, the time from blood sampling to blood culture results is a key determinant of reducing mortality.					
29596458	11	91	theme	Principal	1499:1507	arg1	analysis					1519:1526	Principal component analysis	1499:1526	Principal component analysis	1499:1526	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	10	92	theme	proposed	1471:1478	arg1	structures					1487:1496	proposed glycan structures	1471:1496	proposed glycan structures	1471:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	0	93	theme	various	71:77	arg1	proteins					86:93	various plasma proteins	71:93	various plasma proteins in bloodstream infected patients	71:126	Quantitative N-glycoproteomics reveals altered glycosylation levels of various plasma proteins in bloodstream infected patients.					
29596458	13	94	theme	high-confidence	1785:1799	arg1	N-glycopeptides					1801:1815	high-confidence N-glycopeptides	1785:1815	high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia	1785:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	10	95	theme	composition	1455:1465	arg1	elucidation					1389:1399	elucidation	1389:1399	high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures	1359:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29596458	9	96	gly	N-glycopeptides	1207:1221	arg2	N-glycopeptides					1207:1221	Three hundred and sixty eight potential N-glycopeptides	1167:1221	Three hundred and sixty eight potential N-glycopeptides	1167:1221	Three hundred and sixty eight potential N-glycopeptides were quantified by mass spectrometry and 149 were further selected for identification.					
29596458	8	97	theme	plasma	1014:1019	arg1	samples					1021:1027	plasma samples	1014:1027	plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports	1014:1164	Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports.					
29596458	13	98	dep	power	1832:1836	arg1	separate					1841:1848	separate	1841:1848	separate	1841:1848	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	13	98	dep	power	1832:1836	arg1	shed					1926:1929	shed	1926:1929	shed light on host response during bacteremia	1926:1970	We propose high-confidence N-glycopeptides which have the power to separate the bloodstream infections from blood culture negative febrile patients and shed light on host response during bacteremia.					
29596458	11	99	theme	orthogonal	1529:1538	arg1	projections					1540:1550	orthogonal projections	1529:1550	orthogonal projections to latent structures-discriminant analysis (S-Plot)	1529:1602	Principal component analysis, orthogonal projections to latent structures-discriminant analysis (S-Plot) and self-organizing maps clustering among other statistical methods were employed to analyze the data.					
29596458	10	100	theme	structures	1487:1496	arg1	elucidation					1389:1399	elucidation	1389:1399	high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures	1359:1496	Twenty four N-glycopeptides were identified with high confidence together with elucidation of the peptide sequence, N-glycosylation site, glycan composition and proposed glycan structures.					
29532326	8	0	theme	detailed	1003:1010	arg1	specification					1012:1024	a detailed specification	1001:1024	a detailed specification of co-occurring C8 proteoforms	1001:1055	Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation.					
29532326	4	1	theme	MS	590:591	arg1	signals					593:599	at least ~ 20 co-occurring MS signals	563:599	at least ~ 20 co-occurring MS signals	563:599	The intact C8 proteoform profile revealed at least ~ 20 co-occurring MS signals.					
29532326	5	2	theme	purified	668:675	arg1	C8					677:678	purified C8	668:678	purified C8	668:678	Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions.					
29532326	13	3	from	embedded	1632:1639	arg1	MAC					1648:1650	the MAC	1644:1650	the MAC	1644:1650	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	2	4	theme	membrane	273:280	arg1	penetration					282:292	membrane penetration	273:292	membrane penetration	273:292	C8 initiates membrane penetration and coordinates MAC pore formation.					
29532326	7	5	theme	site-specific	892:904	arg1	profiles					919:926	site-specific quantitative profiles	892:926	site-specific quantitative profiles of different types of C8 glycosylation	892:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	8	6	theme	C8	1042:1043	arg1	proteoforms					1045:1055	co-occurring C8 proteoforms	1029:1055	co-occurring C8 proteoforms	1029:1055	Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation.					
29532326	5	7	theme	exchange	632:639	arg1	chromatography					641:654	ion exchange chromatography	628:654	ion exchange chromatography	628:654	Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions.					
29532326	2	8	theme	MAC	310:312	arg1	formation					319:327	MAC pore formation	310:327	MAC pore formation	310:327	C8 initiates membrane penetration and coordinates MAC pore formation.					
29532326	8	9	theme	experimental	1068:1079	arg1	evidence					1081:1088	experimental evidence	1068:1088	experimental evidence	1068:1088	Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation.					
29532326	1	10	theme	important	202:210	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	10	theme	important	202:210	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	11	11	theme	experimental	1419:1430	arg1	evidence					1432:1439	the first experimental evidence	1409:1439	the first experimental evidence of O-linked glycans located on C8γ	1409:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	9	12	located	detected	1232:1239	arg2	sites					1221:1225	two more N-glycosylation sites	1196:1225	two more N-glycosylation sites	1196:1225	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	9	12	located	detected	1232:1239	arg1	C8					1244:1245	C8	1244:1245	C8	1244:1245	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	13	13	theme	MAC	1745:1747	arg1	formation					1749:1757	the MAC formation	1741:1757	the MAC formation	1741:1757	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	5	14	theme	distinct	690:697	arg1	fractions					699:707	four distinct fractions	685:707	four distinct fractions	685:707	Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions.					
29532326	1	15	theme	hetero-trimeric	140:154	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	15	theme	hetero-trimeric	140:154	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	10	16	theme	sites	1317:1321	arg1	stoichiometry					1280:1292	the stoichiometry	1276:1292	the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β	1276:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	17	from	stoichiometry	1280:1292	arg1	domains					1360:1366	all the thrombospondin-like (TSP) domains	1326:1366	all the thrombospondin-like (TSP) domains of C8α and C8β	1326:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	17	from	stoichiometry	1280:1292	arg1	C8β					1379:1381	C8β	1379:1381	C8β	1379:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	17	from	stoichiometry	1280:1292	arg1	C8α					1371:1373	C8α	1371:1373	C8α	1371:1373	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	9	18	theme	N-glycosylation	1173:1187	arg1	sites					1189:1193	the known N-glycosylation sites	1163:1193	the known N-glycosylation sites	1163:1193	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	13	19	theme	observed	1579:1586	arg1	PTMs					1588:1591	the observed PTMs	1575:1591	the observed PTMs in structural models of free C8 and C8 embedded in the MAC	1575:1650	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	10	20	theme	thrombospondin-like	1334:1352	arg1	domains					1360:1366	all the thrombospondin-like (TSP) domains	1326:1366	all the thrombospondin-like (TSP) domains of C8α and C8β	1326:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	20	theme	thrombospondin-like	1334:1352	arg1	C8β					1379:1381	C8β	1379:1381	C8β	1379:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	20	theme	thrombospondin-like	1334:1352	arg1	C8α					1371:1373	C8α	1371:1373	C8α	1371:1373	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	0	21	theme	Mass	89:92	arg1	Approaches					107:116	Hybrid Mass Spectrometry Approaches	82:116	Hybrid Mass Spectrometry Approaches	82:116	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	13	22	theme	embedded	1632:1639	arg1	PTMs					1588:1591	the observed PTMs	1575:1591	the observed PTMs in structural models of free C8 and C8 embedded in the MAC	1575:1650	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	6	23	theme	MS	723:724	arg1	analysis					726:733	Their native MS analysis	710:733	Their native MS analysis	710:733	Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8.					
29532326	3	24	theme	human	417:421	arg1	plasma					423:428	human plasma	417:428	human plasma	417:428	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	0	25	theme	Comprehensive	0:12	arg1	Characterization					25:40	Comprehensive Proteoform Characterization	0:40	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.	0:117	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	7	26	theme	proteolytic	841:851	arg1	digestion					853:861	proteolytic digestion	841:861	proteolytic digestion of C8 and LC-MS/MS	841:880	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	10	27	theme	C8β	1379:1381	arg1	domains					1360:1366	all the thrombospondin-like (TSP) domains	1326:1366	all the thrombospondin-like (TSP) domains of C8α and C8β	1326:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	27	theme	C8β	1379:1381	arg1	C8β					1379:1381	C8β	1379:1381	C8β	1379:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	27	theme	C8β	1379:1381	arg1	C8α					1371:1373	C8α	1371:1373	C8α	1371:1373	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	3	28	theme	mass	464:467	arg1	spectrometry					469:480	high-resolution native mass spectrometry	441:480	high-resolution native mass spectrometry	441:480	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	1	29	theme	human	123:127	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	29	theme	human	123:127	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	10	30	theme	C8α	1371:1373	arg1	domains					1360:1366	all the thrombospondin-like (TSP) domains	1326:1366	all the thrombospondin-like (TSP) domains of C8α and C8β	1326:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	30	theme	C8α	1371:1373	arg1	C8β					1379:1381	C8β	1379:1381	C8β	1379:1381	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	10	30	theme	C8α	1371:1373	arg1	C8α					1371:1373	C8α	1371:1373	C8α	1371:1373	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	3	31	theme	high-resolution	441:455	arg1	spectrometry					469:480	high-resolution native mass spectrometry	441:480	high-resolution native mass spectrometry	441:480	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	0	32	theme	Plasma	45:50	arg1	C8αβγ					73:77	Plasma Complement Component C8αβγ	45:77	Plasma Complement Component C8αβγ	45:77	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	7	33	theme	peptide-centric	812:826	arg1	analysis					828:835	Subsequent peptide-centric analysis	801:835	Subsequent peptide-centric analysis	801:835	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	4	34	theme	intact	525:530	arg1	profile					546:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile revealed at least ~ 20 co-occurring MS signals.					
29532326	3	35	theme	glyco	487:491	arg1	proteomics					509:518	(glyco)peptide-centric proteomics	486:518	(glyco)peptide-centric proteomics	486:518	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	0	36	theme	Component	63:71	arg1	C8αβγ					73:77	Plasma Complement Component C8αβγ	45:77	Plasma Complement Component C8αβγ	45:77	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	3	37	dep	combining	431:439	arg1	charted					339:345	charted	339:345	charted in detail	339:355	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	4	38	theme	proteoform	535:544	arg1	profile					546:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile revealed at least ~ 20 co-occurring MS signals.					
29532326	6	39	dep	detailed	754:761	arg1	structural					763:772	structural	763:772	structural	763:772	Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8.					
29532326	11	40	theme	O-linked	1444:1451	arg1	glycans					1453:1459	O-linked glycans	1444:1459	O-linked glycans located on C8γ	1444:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	7	41	theme	C8	950:951	arg1	glycosylation					953:965	C8 glycosylation	950:965	C8 glycosylation	950:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	1	42	theme	attack	238:243	arg1	MAC					254:256	MAC	254:256	MAC	254:256	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	42	theme	attack	238:243	arg1	complex					245:251	membrane attack complex	229:251	the membrane attack complex (MAC)	225:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	3	43	theme	peptide-centric	493:507	arg1	proteomics					509:518	(glyco)peptide-centric proteomics	486:518	(glyco)peptide-centric proteomics	486:518	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	7	44	theme	types	941:945	arg1	profiles					919:926	site-specific quantitative profiles	892:926	site-specific quantitative profiles of different types of C8 glycosylation	892:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	9	45	gly	N-glycosylation	1205:1219	arg2	two					1196:1198	two	1196:1198	two	1196:1198	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	9	45	gly	N-glycosylation	1205:1219	arg2	sites					1221:1225	two more N-glycosylation sites	1196:1225	two more N-glycosylation sites	1196:1225	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	8	46	theme	co-occurring	1029:1040	arg1	proteoforms					1045:1055	co-occurring C8 proteoforms	1029:1055	co-occurring C8 proteoforms	1029:1055	Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation.					
29532326	4	47	theme	co-occurring	577:588	arg1	signals					593:599	at least ~ 20 co-occurring MS signals	563:599	at least ~ 20 co-occurring MS signals	563:599	The intact C8 proteoform profile revealed at least ~ 20 co-occurring MS signals.					
29532326	13	48	theme	C8	1629:1630	arg1	models					1607:1612	structural models	1596:1612	structural models of free C8 and C8	1596:1630	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	12	49	located	detected	1538:1545	arg2	O-glycans					1504:1512	these O-glycans	1498:1512	these O-glycans	1498:1512	Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit.					
29532326	12	49	located	detected	1538:1545	arg1	subunit					1555:1561	this subunit	1550:1561	this subunit	1550:1561	Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit.					
29532326	12	49	located	detected	1538:1545	arg2	PTMs					1528:1531	the first PTMs	1518:1531	the first PTMs ever detected on this subunit	1518:1561	Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit.					
29532326	8	50	theme	proteoforms	1045:1055	arg1	specification					1012:1024	a detailed specification	1001:1024	a detailed specification of co-occurring C8 proteoforms	1001:1055	Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation.					
29532326	11	51	theme	located	1461:1467	arg1	glycans					1453:1459	O-linked glycans	1444:1459	O-linked glycans located on C8γ	1444:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	5	52	theme	ion	628:630	arg1	chromatography					641:654	ion exchange chromatography	628:654	ion exchange chromatography	628:654	Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions.					
29532326	7	53	theme	quantitative	906:917	arg1	profiles					919:926	site-specific quantitative profiles	892:926	site-specific quantitative profiles of different types of C8 glycosylation	892:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	2	54	theme	pore	314:317	arg1	formation					319:327	MAC pore formation	310:327	MAC pore formation	310:327	C8 initiates membrane penetration and coordinates MAC pore formation.					
29532326	10	55	gly	C-mannosylation	1301:1315	arg2	sites					1317:1321	all C-mannosylation sites	1297:1321	all C-mannosylation sites	1297:1321	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	13	56	theme	C8	1622:1623	arg1	models					1607:1612	structural models	1596:1612	structural models of free C8 and C8	1596:1630	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	7	57	theme	LC-MS/MS	873:880	arg1	digestion					853:861	proteolytic digestion	841:861	proteolytic digestion of C8 and LC-MS/MS	841:880	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	13	58	from	PTMs	1588:1591	arg1	models					1607:1612	structural models	1596:1612	structural models of free C8 and C8	1596:1630	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	11	59	theme	first	1413:1417	arg1	evidence					1432:1439	the first experimental evidence	1409:1439	the first experimental evidence of O-linked glycans located on C8γ	1409:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	7	60	theme	C8	866:867	arg1	digestion					853:861	proteolytic digestion	841:861	proteolytic digestion of C8 and LC-MS/MS	841:880	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	11	61	contain	contain	1401:1407	arg2	evidence					1432:1439	the first experimental evidence	1409:1439	the first experimental evidence of O-linked glycans located on C8γ	1409:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	11	61	contain	contain	1401:1407	arg1	data					1396:1399	our data	1392:1399	our data	1392:1399	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	13	62	theme	structural	1596:1605	arg1	models					1607:1612	structural models	1596:1612	structural models of free C8 and C8	1596:1630	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	1	63	theme	complement	129:138	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	63	theme	complement	129:138	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	64	theme	C8αβγ	156:160	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	64	theme	C8αβγ	156:160	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	10	65	theme	C-mannosylation	1301:1315	arg1	sites					1317:1321	all C-mannosylation sites	1297:1321	all C-mannosylation sites	1297:1321	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	0	66	theme	Hybrid	82:87	arg1	Approaches					107:116	Hybrid Mass Spectrometry Approaches	82:116	Hybrid Mass Spectrometry Approaches	82:116	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	1	67	theme	C8	163:164	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	67	theme	C8	163:164	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	0	68	theme	Spectrometry	94:105	arg1	Approaches					107:116	Hybrid Mass Spectrometry Approaches	82:116	Hybrid Mass Spectrometry Approaches	82:116	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	1	69	theme	protein	167:173	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	69	theme	protein	167:173	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	10	70	dep	thrombospondin-like	1334:1352	arg1	TSP					1355:1357	TSP	1355:1357	TSP	1355:1357	Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β.					
29532326	14	71	theme	Graphical	1760:1768	arg1	Abstract					1770:1777	Graphical Abstract	1760:1777	Graphical Abstract	1760:1777	Graphical Abstract ᅟ.					
29532326	6	72	theme	native	716:721	arg1	analysis					726:733	Their native MS analysis	710:733	Their native MS analysis	710:733	Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8.					
29532326	0	73	theme	Proteoform	14:23	arg1	Characterization					25:40	Comprehensive Proteoform Characterization	0:40	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.	0:117	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	13	74	theme	identified	1697:1706	arg1	modifications					1708:1720	the newly identified modifications	1687:1720	the newly identified modifications	1687:1720	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	9	75	theme	known	1167:1171	arg1	sites					1189:1193	the known N-glycosylation sites	1163:1193	the known N-glycosylation sites	1163:1193	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	11	76	link	O-linked	1444:1451	arg1	glycans					1453:1459	O-linked glycans	1444:1459	O-linked glycans located on C8γ	1444:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	3	77	theme	structural	361:370	arg1	micro-heterogeneity					372:390	the structural micro-heterogeneity	357:390	the structural micro-heterogeneity	357:390	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	0	78	theme	Complement	52:61	arg1	C8αβγ					73:77	Plasma Complement Component C8αβγ	45:77	Plasma Complement Component C8αβγ	45:77	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	4	79	theme	C8	532:533	arg1	profile					546:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile	521:552	The intact C8 proteoform profile revealed at least ~ 20 co-occurring MS signals.					
29532326	13	80	from	MAC	1648:1650	arg1	embedded					1632:1639	embedded	1632:1639	embedded	1632:1639	By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.					
29532326	3	81	theme	native	457:462	arg1	spectrometry					469:480	high-resolution native mass spectrometry	441:480	high-resolution native mass spectrometry	441:480	Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics.					
29532326	0	82	theme	C8αβγ	73:77	arg1	Characterization					25:40	Comprehensive Proteoform Characterization	0:40	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.	0:117	Comprehensive Proteoform Characterization of Plasma Complement Component C8αβγ by Hybrid Mass Spectrometry Approaches.					
29532326	9	83	gly	N-glycosylation	1173:1187	arg2	sites					1189:1193	the known N-glycosylation sites	1163:1193	the known N-glycosylation sites	1163:1193	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	6	84	from	micro-heterogeneity	774:792	arg1	C8					797:798	C8	797:798	C8	797:798	Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8.					
29532326	9	85	theme	N-glycosylation	1205:1219	arg1	sites					1221:1225	two more N-glycosylation sites	1196:1225	two more N-glycosylation sites	1196:1225	In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8.					
29532326	7	86	theme	glycosylation	953:965	arg1	types					941:945	different types	931:945	different types of C8 glycosylation	931:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	7	87	theme	Subsequent	801:810	arg1	analysis					828:835	Subsequent peptide-centric analysis	801:835	Subsequent peptide-centric analysis	801:835	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	12	88	theme	first	1522:1526	arg1	O-glycans					1504:1512	these O-glycans	1498:1512	these O-glycans	1498:1512	Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit.					
29532326	12	88	theme	first	1522:1526	arg1	PTMs					1528:1531	the first PTMs	1518:1531	the first PTMs ever detected on this subunit	1518:1561	Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit.					
29532326	6	89	theme	detailed	754:761	arg1	micro-heterogeneity					774:792	even more detailed structural micro-heterogeneity	744:792	even more detailed structural micro-heterogeneity on C8	744:798	Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8.					
29532326	1	90	theme	membrane	229:236	arg1	MAC					254:256	MAC	254:256	MAC	254:256	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	90	theme	membrane	229:236	arg1	complex					245:251	membrane attack complex	229:251	the membrane attack complex (MAC)	225:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	11	91	theme	glycans	1453:1459	arg1	evidence					1432:1439	the first experimental evidence	1409:1439	the first experimental evidence of O-linked glycans located on C8γ	1409:1474	Lastly, our data contain the first experimental evidence of O-linked glycans located on C8γ.					
29532326	7	92	theme	different	931:939	arg1	types					941:945	different types	931:945	different types of C8 glycosylation	931:965	Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation.					
29532326	1	93	theme	complex	245:251	arg1	component					212:220	an important component	199:220	an important component of the membrane attack complex (MAC)	199:257	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29532326	1	93	theme	complex	245:251	arg1	assembly					175:182	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa)	119:194	The human complement hetero-trimeric C8αβγ (C8) protein assembly (~ 150 kDa) is an important component of the membrane attack complex (MAC).					
29408166	4	0	theme	consecutive	921:931	arg1	deglycosylation					958:972	consecutive two-step on-bead partial deglycosylation	921:972	consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	921:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	3	1	from	serum	722:726	arg1	analysis					642:649	the simultaneous analysis	625:649	the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range	625:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	10	2	from	markers	2039:2045	arg1	research					2079:2086	cancer research	2072:2086	cancer research	2072:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	8	3	from	method	1524:1529	arg1	range					1605:1609	the low ng/ml concentration range	1577:1609	the low ng/ml concentration range in human serum	1577:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	4	4	theme	on-bead	942:948	arg1	deglycosylation					958:972	consecutive two-step on-bead partial deglycosylation	921:972	consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	921:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	3	5	from	antigen	699:705	arg1	range					759:763	the low ng/ml concentration range	731:763	the low ng/ml concentration range	731:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	5	from	antigen	699:705	arg1	serum					722:726	human serum	716:726	human serum	716:726	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	10	6	theme	glycoproteins	1989:2001	arg1	core-fucosylation					1945:1961	site-specific core-fucosylation	1931:1961	site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research	1931:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	4	7	theme	magnetic	894:901	arg1	beads					903:907	anti-PSA antibody coated magnetic beads	869:907	anti-PSA antibody coated magnetic beads	869:907	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	7	8	theme	human	1462:1466	arg1	specimens					1468:1476	three human specimens	1456:1476	three human specimens	1456:1476	The feasibility of the method in native sera was shown using three human specimens.					
29408166	3	9	theme	concentration	745:757	arg1	range					759:763	the low ng/ml concentration range	731:763	the low ng/ml concentration range	731:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	6	10	theme	core-fucosylated	1317:1332	arg1	PSA					1334:1336	core-fucosylated PSA	1317:1336	core-fucosylated PSA	1317:1336	The imprecision of the method over two days ranged from 9.7-23.2% for core-fucosylated PSA and 10.3-18.3% for total PSA depending on the PSA level.					
29408166	10	11	used	used	1894:1897	arg2	strategy					1876:1883	the described strategy	1862:1883	the described strategy	1862:1883	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	9	12	theme	prostate	1759:1766	arg1	cancer					1768:1773	prostate cancer	1759:1773	prostate cancer	1759:1773	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	3	13	gly	core-fucosylated	654:669	arg1	PSA					708:710	PSA	708:710	PSA	708:710	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	13	gly	core-fucosylated	654:669	arg1	antigen					699:705	core-fucosylated and total prostate-specific antigen	654:705	antigen	699:705	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	14	from	range	759:763	arg1	analysis					642:649	the simultaneous analysis	625:649	the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range	625:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	1	15	gly	glycoproteins	243:255	arg1	glycoproteins					243:255	glycoproteins	243:255	glycoproteins	243:255	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	1	16	theme	site-specific	213:225	arg1	fucosylation					227:238	site-specific fucosylation	213:238	site-specific fucosylation of glycoproteins	213:255	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	8	17	theme	core-fucosylated	1553:1568	arg1	PSA					1570:1572	core-fucosylated PSA	1553:1572	core-fucosylated PSA	1553:1572	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	10	18	gly	glycoproteins	1989:2001	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	18	gly	glycoproteins	1989:2001	arg1	glycoproteins					1989:2001	other low-concentrated glycoproteins	1966:2001	other low-concentrated glycoproteins	1966:2001	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	7	19	theme	native	1428:1433	arg1	sera					1435:1438	native sera	1428:1438	native sera	1428:1438	The feasibility of the method in native sera was shown using three human specimens.					
29408166	5	20	theme	simultaneous	1143:1154	arg1	quantification					1156:1169	the simultaneous quantification	1139:1169	the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml	1139:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	7	21	theme	method	1418:1423	arg1	feasibility					1399:1409	The feasibility	1395:1409	The feasibility of the method in native sera	1395:1438	The feasibility of the method in native sera was shown using three human specimens.					
29408166	9	22	theme	cancer	1768:1773	arg1	differentiation					1740:1754	the differentiation	1736:1754	the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH)	1736:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	5	23	theme	total	1103:1107	arg1	concentrations					1113:1126	0.5 to 60 ng/ml total PSA concentrations	1087:1126	0.5 to 60 ng/ml total PSA concentrations	1087:1126	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	9	24	theme	core-fucosylated	1681:1696	arg1	biomarker					1722:1730	a diagnostic biomarker	1709:1730	a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH)	1709:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	24	theme	core-fucosylated	1681:1696	arg1	PSA					1698:1700	core-fucosylated PSA	1681:1700	core-fucosylated PSA	1681:1700	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	4	25	theme	endoglycosidase	979:993	arg1	F3					995:996	endoglycosidase F3	979:996	endoglycosidase F3	979:996	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	4	26	theme	Sample	766:771	arg1	preparation					773:783	Sample preparation	766:783	Sample preparation	766:783	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	10	27	theme	refinement	2056:2065	arg1	"					2066:2066	"marker refinement"	2048:2066	"marker refinement"	2048:2066	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	27	theme	refinement	2056:2065	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	6	28	theme	total	1357:1361	arg1	PSA					1363:1365	total PSA	1357:1365	total PSA	1357:1365	The imprecision of the method over two days ranged from 9.7-23.2% for core-fucosylated PSA and 10.3-18.3% for total PSA depending on the PSA level.					
29408166	3	29	theme	total	675:679	arg1	PSA					708:710	PSA	708:710	PSA	708:710	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	29	theme	total	675:679	arg1	antigen					699:705	core-fucosylated and total prostate-specific antigen	654:705	antigen	699:705	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	9	30	theme	other	1779:1783	arg1	diseases					1795:1802	other prostatic diseases	1779:1802	other prostatic diseases	1779:1802	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	30	theme	other	1779:1783	arg1	hyperplasia					1830:1840	benign prostatic hyperplasia	1813:1840	benign prostatic hyperplasia (BPH)	1813:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	8	31	theme	first	1509:1513	arg1	method					1524:1529	the first MS-based method	1505:1529	the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum	1505:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	8	31	theme	first	1509:1513	arg1	this					1497:1500	this	1497:1500	this	1497:1500	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	6	32	theme	method	1270:1275	arg1	imprecision					1251:1261	The imprecision	1247:1261	The imprecision of the method over two days	1247:1289	The imprecision of the method over two days ranged from 9.7-23.2% for core-fucosylated PSA and 10.3-18.3% for total PSA depending on the PSA level.					
29408166	0	33	theme	site-specific	72:84	arg1	core-fucosylation					86:102	site-specific core-fucosylation	72:102	site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example	72:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	3	34	theme	core-fucosylated	654:669	arg1	PSA					708:710	PSA	708:710	PSA	708:710	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	34	theme	core-fucosylated	654:669	arg1	antigen					699:705	core-fucosylated and total prostate-specific antigen	654:705	antigen	699:705	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	10	35	gly	core-fucosylation	1945:1961	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	35	gly	core-fucosylation	1945:1961	arg1	glycoproteins					1989:2001	other low-concentrated glycoproteins	1966:2001	other low-concentrated glycoproteins	1966:2001	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	9	36	theme	diseases	1795:1802	arg1	differentiation					1740:1754	the differentiation	1736:1754	the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH)	1736:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	2	37	from	low-concentrated	459:474	arg1	matrices					493:500	complex serum matrices	479:500	complex serum matrices	479:500	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	10	38	theme	cancer	2072:2077	arg1	research					2079:2086	cancer research	2072:2086	cancer research	2072:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	0	39	theme	glycoproteins	124:136	arg1	core-fucosylation					86:102	site-specific core-fucosylation	72:102	site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example	72:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	0	40	gly	core-fucosylation	86:102	arg1	glycoproteins					124:136	low-concentrated glycoproteins	107:136	low-concentrated glycoproteins	107:136	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	0	40	gly	core-fucosylation	86:102	arg1	serum					147:151	human serum	141:151	human serum using prostate-specific antigen (PSA) as example	141:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	9	41	theme	benign	1813:1818	arg1	BPH					1843:1845	BPH	1843:1845	BPH	1843:1845	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	41	theme	benign	1813:1818	arg1	hyperplasia					1830:1840	benign prostatic hyperplasia	1813:1840	benign prostatic hyperplasia (BPH)	1813:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	0	42	from	analysis	60:67	arg1	serum					147:151	human serum	141:151	human serum using prostate-specific antigen (PSA) as example	141:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	0	43	theme	human	141:145	arg1	serum					147:151	human serum	141:151	human serum using prostate-specific antigen (PSA) as example	141:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	5	44	theme	PSA	1191:1193	arg1	quantification					1156:1169	the simultaneous quantification	1139:1169	the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml	1139:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	2	45	theme	serum	487:491	arg1	matrices					493:500	complex serum matrices	479:500	complex serum matrices	479:500	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	0	46	theme	core-fucosylation	86:102	arg1	analysis					60:67	the analysis	56:67	the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example	56:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	2	47	theme	potential	418:426	arg1	markers					435:441	potential cancer markers	418:441	potential cancer markers	418:441	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	2	47	theme	potential	418:426	arg1	glycoproteins					382:394	individual glycoproteins	371:394	individual glycoproteins	371:394	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	1	48	theme	several	310:316	arg1	types					318:322	several types	310:322	several types of cancers including prostate cancer	310:359	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	8	49	theme	low	1581:1583	arg1	range					1605:1609	the low ng/ml concentration range	1577:1609	the low ng/ml concentration range in human serum	1577:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	4	50	theme	immunoaffinity	798:811	arg1	step					821:824	an immunoaffinity capture step	795:824	an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	795:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	0	51	theme	LC-MS/MS-based	28:41	arg1	strategy					43:50	An endoglycosidase-assisted LC-MS/MS-based strategy	0:50	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.	0:201	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	4	52	with	deglycosylation	958:972	arg1	F3					995:996	endoglycosidase F3	979:996	endoglycosidase F3	979:996	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	4	52	with	deglycosylation	958:972	arg1	digestion					1010:1018	tryptic digestion	1002:1018	tryptic digestion	1002:1018	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	2	53	theme	glycan	515:520	arg1	structures					522:531	distinct glycan structures	506:531	distinct glycan structures	506:531	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	8	54	theme	concentration	1591:1603	arg1	range					1605:1609	the low ng/ml concentration range	1577:1609	the low ng/ml concentration range in human serum	1577:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	3	55	from	analysis	642:649	arg1	range					759:763	the low ng/ml concentration range	731:763	the low ng/ml concentration range	731:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	55	from	analysis	642:649	arg1	serum					722:726	human serum	716:726	human serum	716:726	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	56	theme	antigen	699:705	arg1	analysis					642:649	the simultaneous analysis	625:649	the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range	625:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	4	57	theme	antibody	878:885	arg1	beads					903:907	anti-PSA antibody coated magnetic beads	869:907	anti-PSA antibody coated magnetic beads	869:907	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	10	58	theme	other	1966:1970	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	58	theme	other	1966:1970	arg1	glycoproteins					1989:2001	other low-concentrated glycoproteins	1966:2001	other low-concentrated glycoproteins	1966:2001	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	1	59	gly	fucosylation	227:238	arg1	glycoproteins					243:255	glycoproteins	243:255	glycoproteins	243:255	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	4	60	theme	human	851:855	arg1	serum					857:861	human serum	851:861	human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	851:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	9	61	gly	core-fucosylated	1681:1696	arg1	biomarker					1722:1730	a diagnostic biomarker	1709:1730	a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH)	1709:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	61	gly	core-fucosylated	1681:1696	arg1	PSA					1698:1700	core-fucosylated PSA	1681:1700	core-fucosylated PSA	1681:1700	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	62	theme	patient	1662:1668	arg1	cohorts					1670:1676	large patient cohorts	1656:1676	large patient cohorts	1656:1676	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	5	63	theme	lower	1225:1229	arg1	PSA					1221:1223	total PSA	1215:1223	total PSA lower than 0.5 ng/ml	1215:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	3	64	theme	ng/ml	739:743	arg1	range					759:763	the low ng/ml concentration range	731:763	the low ng/ml concentration range	731:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	8	65	from	range	1605:1609	arg1	serum					1620:1624	human serum	1614:1624	human serum	1614:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	10	66	theme	described	1866:1874	arg1	strategy					1876:1883	the described strategy	1862:1883	the described strategy	1862:1883	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	4	67	theme	two-step	933:940	arg1	deglycosylation					958:972	consecutive two-step on-bead partial deglycosylation	921:972	consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	921:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	10	68	theme	low-concentrated	1972:1987	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	68	theme	low-concentrated	1972:1987	arg1	glycoproteins					1989:2001	other low-concentrated glycoproteins	1966:2001	other low-concentrated glycoproteins	1966:2001	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	4	69	theme	coated	887:892	arg1	beads					903:907	anti-PSA antibody coated magnetic beads	869:907	anti-PSA antibody coated magnetic beads	869:907	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	5	70	dep	60 ng/ml	1094:1101	arg1	to					1091:1092	to	1091:1092	to	1091:1092	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	3	71	theme	spectrometry-based	593:610	arg1	strategy					612:619	a mass spectrometry-based strategy	586:619	a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range	586:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	10	72	from	changes	1920:1926	arg1	core-fucosylation					1945:1961	site-specific core-fucosylation	1931:1961	site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research	1931:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	9	73	theme	diagnostic	1711:1720	arg1	biomarker					1722:1730	a diagnostic biomarker	1709:1730	a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH)	1709:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	73	theme	diagnostic	1711:1720	arg1	PSA					1698:1700	core-fucosylated PSA	1681:1700	core-fucosylated PSA	1681:1700	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	10	74	theme	potential	1910:1918	arg1	changes					1920:1926	potential changes	1910:1926	potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research	1910:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	5	75	gly	core-fucosylated	1174:1189	arg1	PSA					1191:1193	core-fucosylated PSA	1174:1193	core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml	1174:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	0	76	theme	prostate-specific	159:175	arg1	antigen					177:183	prostate-specific antigen	159:183	prostate-specific antigen (PSA)	159:189	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	0	76	theme	prostate-specific	159:175	arg1	PSA					186:188	PSA	186:188	PSA	186:188	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	6	77	theme	PSA	1384:1386	arg1	level					1388:1392	the PSA level	1380:1392	the PSA level	1380:1392	The imprecision of the method over two days ranged from 9.7-23.2% for core-fucosylated PSA and 10.3-18.3% for total PSA depending on the PSA level.					
29408166	3	78	theme	simultaneous	629:640	arg1	analysis					642:649	the simultaneous analysis	625:649	the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range	625:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	4	79	dep	using	863:867	arg1	followed					909:916	followed	909:916	followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	909:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	4	80	theme	tryptic	1002:1008	arg1	digestion					1010:1018	tryptic digestion	1002:1018	tryptic digestion	1002:1018	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	0	81	from	core-fucosylation	86:102	arg1	serum					147:151	human serum	141:151	human serum using prostate-specific antigen (PSA) as example	141:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	10	82	theme	specific	2030:2037	arg1	glycoproteins					1989:2001	other low-concentrated glycoproteins	1966:2001	other low-concentrated glycoproteins	1966:2001	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	82	theme	specific	2030:2037	arg1	"					2066:2066	"marker refinement"	2048:2066	"marker refinement"	2048:2066	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	82	theme	specific	2030:2037	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	6	83	gly	core-fucosylated	1317:1332	arg1	PSA					1334:1336	core-fucosylated PSA	1317:1336	core-fucosylated PSA	1317:1336	The imprecision of the method over two days ranged from 9.7-23.2% for core-fucosylated PSA and 10.3-18.3% for total PSA depending on the PSA level.					
29408166	4	84	theme	partial	950:956	arg1	deglycosylation					958:972	consecutive two-step on-bead partial deglycosylation	921:972	consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	921:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	0	85	from	serum	147:151	arg1	analysis					60:67	the analysis	56:67	the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example	56:200	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	5	86	theme	PSA	1109:1111	arg1	concentrations					1113:1126	0.5 to 60 ng/ml total PSA concentrations	1087:1126	0.5 to 60 ng/ml total PSA concentrations	1087:1126	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	4	87	from	serum	857:861	arg1	PSA					842:844	total PSA	836:844	total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	836:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	8	88	theme	MS-based	1515:1522	arg1	method					1524:1529	the first MS-based method	1505:1529	the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum	1505:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	8	88	theme	MS-based	1515:1522	arg1	this					1497:1500	this	1497:1500	this	1497:1500	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	10	89	theme	marker	2049:2054	arg1	"					2066:2066	"marker refinement"	2048:2066	"marker refinement"	2048:2066	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	10	89	theme	marker	2049:2054	arg1	markers					2039:2045	more specific markers	2025:2045	more specific markers ("marker refinement") in cancer research	2025:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	5	90	from	concentrations	1113:1126	arg1	linear					1075:1080	linear	1075:1080	linear	1075:1080	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	8	91	gly	core-fucosylated	1553:1568	arg1	PSA					1570:1572	core-fucosylated PSA	1553:1572	core-fucosylated PSA	1553:1572	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	9	92	theme	prostatic	1785:1793	arg1	diseases					1795:1802	other prostatic diseases	1779:1802	other prostatic diseases	1779:1802	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	92	theme	prostatic	1785:1793	arg1	hyperplasia					1830:1840	benign prostatic hyperplasia	1813:1840	benign prostatic hyperplasia (BPH)	1813:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	0	93	theme	low-concentrated	107:122	arg1	glycoproteins					124:136	low-concentrated glycoproteins	107:136	low-concentrated glycoproteins	107:136	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	5	94	theme	total	1215:1219	arg1	PSA					1221:1223	total PSA	1215:1223	total PSA lower than 0.5 ng/ml	1215:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	4	95	theme	prior	1020:1024	arg1	F3					995:996	endoglycosidase F3	979:996	endoglycosidase F3	979:996	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	8	96	theme	PSA	1570:1572	arg1	quantification					1535:1548	quantification	1535:1548	quantification of core-fucosylated PSA	1535:1572	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	4	97	theme	capture	813:819	arg1	step					821:824	an immunoaffinity capture step	795:824	an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	795:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	5	98	theme	core-fucosylated	1174:1189	arg1	PSA					1191:1193	core-fucosylated PSA	1174:1193	core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml	1174:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	7	99	from	feasibility	1399:1409	arg1	sera					1435:1438	native sera	1428:1438	native sera	1428:1438	The feasibility of the method in native sera was shown using three human specimens.					
29408166	4	100	theme	LC-MS/MS	1029:1036	arg1	analysis					1038:1045	LC-MS/MS analysis	1029:1045	LC-MS/MS analysis	1029:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	2	101	theme	cancer	428:433	arg1	markers					435:441	potential cancer markers	418:441	potential cancer markers	418:441	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	2	101	theme	cancer	428:433	arg1	glycoproteins					382:394	individual glycoproteins	371:394	individual glycoproteins	371:394	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	0	102	theme	endoglycosidase-assisted	3:26	arg1	strategy					43:50	An endoglycosidase-assisted LC-MS/MS-based strategy	0:50	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.	0:201	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	5	103	theme	down	1195:1198	arg1	PSA					1191:1193	core-fucosylated PSA	1174:1193	core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml	1174:1244	The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml.					
29408166	10	104	theme	site-specific	1931:1943	arg1	core-fucosylation					1945:1961	site-specific core-fucosylation	1931:1961	site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research	1931:2086	Furthermore, the described strategy could be used to monitor potential changes in site-specific core-fucosylation of other low-concentrated glycoproteins, which could serve as more specific markers ("marker refinement") in cancer research.					
29408166	0	105	gly	glycoproteins	124:136	arg1	glycoproteins					124:136	low-concentrated glycoproteins	107:136	low-concentrated glycoproteins	107:136	An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example.					
29408166	2	106	from	matrices	493:500	arg1	low-concentrated					459:474	low-concentrated	459:474	low-concentrated	459:474	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	2	107	theme	complex	479:485	arg1	matrices					493:500	complex serum matrices	479:500	complex serum matrices	479:500	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	8	108	theme	ng/ml	1585:1589	arg1	range					1605:1609	the low ng/ml concentration range	1577:1609	the low ng/ml concentration range in human serum	1577:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	1	109	theme	cancers	327:333	arg1	types					318:322	several types	310:322	several types of cancers including prostate cancer	310:359	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	1	110	theme	glycoproteins	243:255	arg1	fucosylation					227:238	site-specific fucosylation	213:238	site-specific fucosylation of glycoproteins	213:255	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	2	111	theme	distinct	506:513	arg1	structures					522:531	distinct glycan structures	506:531	distinct glycan structures	506:531	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	8	112	theme	human	1614:1618	arg1	serum					1620:1624	human serum	1614:1624	human serum	1614:1624	To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum.					
29408166	3	113	theme	prostate-specific	681:697	arg1	PSA					708:710	PSA	708:710	PSA	708:710	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	3	113	theme	prostate-specific	681:697	arg1	antigen					699:705	core-fucosylated and total prostate-specific antigen	654:705	antigen	699:705	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	9	114	theme	prostatic	1820:1828	arg1	BPH					1843:1845	BPH	1843:1845	BPH	1843:1845	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	9	114	theme	prostatic	1820:1828	arg1	hyperplasia					1830:1840	benign prostatic hyperplasia	1813:1840	benign prostatic hyperplasia (BPH)	1813:1846	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	1	115	theme	prostate	345:352	arg1	cancer					354:359	prostate cancer	345:359	prostate cancer	345:359	Recently, site-specific fucosylation of glycoproteins has attracted attention as it can be associated with several types of cancers including prostate cancer.					
29408166	2	116	gly	glycoproteins	382:394	arg1	glycoproteins					382:394	individual glycoproteins	371:394	individual glycoproteins	371:394	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	2	116	gly	glycoproteins	382:394	arg1	markers					435:441	potential cancer markers	418:441	potential cancer markers	418:441	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	3	117	theme	human	716:720	arg1	serum					722:726	human serum	716:726	human serum	716:726	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29408166	4	118	theme	anti-PSA	869:876	arg1	beads					903:907	anti-PSA antibody coated magnetic beads	869:907	anti-PSA antibody coated magnetic beads	869:907	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	2	119	theme	individual	371:380	arg1	glycoproteins					382:394	individual glycoproteins	371:394	individual glycoproteins	371:394	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	2	119	theme	individual	371:380	arg1	markers					435:441	potential cancer markers	418:441	potential cancer markers	418:441	However, individual glycoproteins, which might serve as potential cancer markers, often are very low-concentrated in complex serum matrices and distinct glycan structures are hard to detect by immunoassays.					
29408166	9	120	used	used	1648:1651	arg2	method					1632:1637	This method	1627:1637	This method	1627:1637	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	4	121	theme	total	836:840	arg1	PSA					842:844	total PSA	836:844	total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis	836:1045	Sample preparation comprised an immunoaffinity capture step to enrich total PSA from human serum using anti-PSA antibody coated magnetic beads followed by consecutive two-step on-bead partial deglycosylation with endoglycosidase F3 and tryptic digestion prior to LC-MS/MS analysis.					
29408166	9	122	theme	large	1656:1660	arg1	cohorts					1670:1676	large patient cohorts	1656:1676	large patient cohorts	1656:1676	This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH).					
29408166	3	123	theme	low	735:737	arg1	range					759:763	the low ng/ml concentration range	731:763	the low ng/ml concentration range	731:763	Here, we present a mass spectrometry-based strategy for the simultaneous analysis of core-fucosylated and total prostate-specific antigen (PSA) in human serum in the low ng/ml concentration range.					
29587225	0	0	theme	erythropoietin	85:98	arg1	dynamics					55:62	protein dynamics	47:62	protein dynamics of recombinant human erythropoietin	47:98	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	3	1	from	understanding	407:419	arg1	conformation					458:469	EPO conformation	454:469	EPO conformation	454:469	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	3	1	from	understanding	407:419	arg1	dynamics					475:482	dynamics	475:482	dynamics	475:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	2	2	contain	carry	244:248	arg1	glycans					236:242	EPO glycans	232:242	EPO glycans	232:242	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	2	2	contain	carry	244:248	arg2	sialylation					260:270	extensive sialylation	250:270	extensive sialylation	250:270	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	0	3	theme	human	79:83	arg1	erythropoietin					85:98	recombinant human erythropoietin	67:98	recombinant human erythropoietin	67:98	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	2	4	theme	protein	343:349	arg1	stability					351:359	protein stability	343:359	protein stability	343:359	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	4	5	theme	terminal	582:589	arg1	acid					598:601	terminal sialic acid	582:601	terminal sialic acid	582:601	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	5	6	theme	glycosylation	749:761	arg1	sites					763:767	the glycosylation sites	745:767	the glycosylation sites	745:767	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	1	7	gly	glycoprotein	186:197	arg1	glycoprotein					186:197	a therapeutic glycoprotein	172:197	a therapeutic glycoprotein widely used for treating anemia	172:229	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	7	gly	glycoprotein	186:197	arg1	erythropoietin					148:161	Recombinant human erythropoietin	130:161	Recombinant human erythropoietin (EPO)	130:167	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	7	8	theme	glycosylation	1160:1172	arg1	type					1152:1155	specific type	1143:1155	specific type of glycosylation	1143:1172	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	4	9	theme	acid	598:601	arg1	trimming					570:577	enzymatic trimming	560:577	enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	560:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	7	10	from	role	1215:1218	arg1	therapeutics					1246:1257	protein therapeutics	1238:1257	protein therapeutics	1238:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	7	11	theme	specific	1143:1150	arg1	type					1152:1155	specific type	1143:1155	specific type of glycosylation	1143:1172	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	0	12	dep	N-	105:106	arg1	desialylation					115:127	desialylation	115:127	desialylation	115:127	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	1	13	theme	therapeutic	174:184	arg1	glycoprotein					186:197	a therapeutic glycoprotein	172:197	a therapeutic glycoprotein widely used for treating anemia	172:229	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	13	theme	therapeutic	174:184	arg1	erythropoietin					148:161	Recombinant human erythropoietin	130:161	Recombinant human erythropoietin (EPO)	130:167	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	3	14	theme	sialylation	439:449	arg1	effects					428:434	the effects	424:434	the effects of sialylation on EPO conformation and dynamics	424:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	5	15	theme	relative	832:839	arg1	site					827:830	the O-glycosylation site	807:830	the O-glycosylation site relative to the N-glycosylation sites	807:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	6	16	theme	helix	933:937	arg1	bundle					939:944	the helix bundle	929:944	the helix bundle	929:944	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	4	17	theme	sialic	591:596	arg1	acid					598:601	terminal sialic acid	582:601	terminal sialic acid	582:601	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	18	theme	amide	606:610	arg1	HDX-MS					659:664	HDX-MS	659:664	HDX-MS	659:664	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	18	theme	amide	606:610	arg1	spectrometry					645:656	amide hydrogen deuterium exchange mass spectrometry	606:656	amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	606:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	0	19	theme	deuterium	9:17	arg1	exchange					19:26	Hydrogen deuterium exchange	0:26	Hydrogen deuterium exchange	0:26	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	5	20	theme	greater	775:781	arg1	effects					783:789	greater effects	775:789	greater effects observed around the O-glycosylation site relative to the N-glycosylation sites	775:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	3	21	theme	effects	428:434	arg1	understanding					407:419	a detailed understanding	396:419	a detailed understanding of the effects of sialylation on EPO conformation and dynamics	396:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	0	22	theme	Hydrogen	0:7	arg1	exchange					19:26	Hydrogen deuterium exchange	0:26	Hydrogen deuterium exchange	0:26	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	4	23	theme	enzymatic	560:568	arg1	trimming					570:577	enzymatic trimming	560:577	enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	560:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	5	24	gly	glycosylation	749:761	arg2	sites					763:767	the glycosylation sites	745:767	the glycosylation sites	745:767	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	5	25	theme	N-glycosylation	848:862	arg1	sites					864:868	the N-glycosylation sites	844:868	the N-glycosylation sites	844:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	6	26	theme	molecule	1004:1011	arg1	thermostability					981:995	the thermostability	977:995	the thermostability of the molecule	977:1011	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	5	27	theme	structural	717:726	arg1	flexibility					728:738	structural flexibility	717:738	structural flexibility	717:738	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	7	28	theme	type	1152:1155	arg1	function					1131:1138	the function	1127:1138	the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics	1127:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	2	29	theme	modification	293:304	arg1	level					280:284	the level	276:284	the level of the modification	276:304	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	4	30	theme	mass	640:643	arg1	HDX-MS					659:664	HDX-MS	659:664	HDX-MS	659:664	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	30	theme	mass	640:643	arg1	spectrometry					645:656	amide hydrogen deuterium exchange mass spectrometry	606:656	amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	606:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	7	31	theme	technique	1102:1110	arg1	feasibility					1080:1090	the feasibility	1076:1090	the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics	1076:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	7	32	theme	sialylation	1223:1233	arg1	role					1215:1218	the role	1211:1218	the role of sialylation on protein therapeutics	1211:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	3	33	theme	EPO	454:456	arg1	conformation					458:469	EPO conformation	454:469	EPO conformation	454:469	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	5	34	theme	O-glycosylation	811:825	arg1	site					827:830	the O-glycosylation site	807:830	the O-glycosylation site relative to the N-glycosylation sites	807:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	6	35	theme	melting	1030:1036	arg1	measurement					1038:1048	melting measurement	1030:1048	melting measurement	1030:1048	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	6	36	theme	surface-exposed	896:910	arg1	regions					884:890	The affected regions	871:890	The affected regions	871:890	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	6	36	theme	surface-exposed	896:910	arg1	loops					912:916	surface-exposed loops	896:916	surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement	896:1048	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	2	37	theme	EPO	232:234	arg1	glycans					236:242	EPO glycans	232:242	EPO glycans	232:242	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	7	38	theme	protein	1238:1244	arg1	therapeutics					1246:1257	protein therapeutics	1238:1257	protein therapeutics	1238:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	3	39	theme	detailed	398:405	arg1	understanding					407:419	a detailed understanding	396:419	a detailed understanding of the effects of sialylation on EPO conformation and dynamics	396:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	3	40	from	conformation	458:469	arg1	understanding					407:419	a detailed understanding	396:419	a detailed understanding of the effects of sialylation on EPO conformation and dynamics	396:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	0	41	theme	protein	47:53	arg1	dynamics					55:62	protein dynamics	47:62	protein dynamics of recombinant human erythropoietin	47:98	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	7	42	theme	HDX-MS	1095:1100	arg1	technique					1102:1110	HDX-MS technique	1095:1110	HDX-MS technique	1095:1110	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	5	43	gly	N-glycosylation	848:862	arg2	sites					864:868	the N-glycosylation sites	844:868	the N-glycosylation sites	844:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	4	44	theme	EPO	537:539	arg1	dynamics					541:548	EPO dynamics	537:548	EPO dynamics	537:548	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	7	45	theme	novel	1191:1195	arg1	insights					1197:1204	novel insights	1191:1204	novel insights into the role of sialylation on protein therapeutics	1191:1257	Our findings demonstrate the feasibility of HDX-MS technique in deciphering the function of specific type of glycosylation that can provide novel insights into the role of sialylation on protein therapeutics.					
29587225	6	46	theme	affected	875:882	arg1	regions					884:890	The affected regions	871:890	The affected regions	871:890	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	6	46	theme	affected	875:882	arg1	loops					912:916	surface-exposed loops	896:916	surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement	896:1048	The affected regions are surface-exposed loops connecting the helix bundle, which do not appear to reduce the thermostability of the molecule as revealed from melting measurement.					
29587225	5	47	gly	O-glycosylation	811:825	arg2	site					827:830	the O-glycosylation site	807:830	the O-glycosylation site relative to the N-glycosylation sites	807:868	The results revealed that desialylation enhances structural flexibility near the glycosylation sites, with greater effects observed around the O-glycosylation site relative to the N-glycosylation sites.					
29587225	3	48	from	dynamics	475:482	arg1	understanding					407:419	a detailed understanding	396:419	a detailed understanding of the effects of sialylation on EPO conformation and dynamics	396:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	1	49	theme	Recombinant	130:140	arg1	glycoprotein					186:197	a therapeutic glycoprotein	172:197	a therapeutic glycoprotein widely used for treating anemia	172:229	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	49	theme	Recombinant	130:140	arg1	EPO					164:166	EPO	164:166	EPO	164:166	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	49	theme	Recombinant	130:140	arg1	erythropoietin					148:161	Recombinant human erythropoietin	130:161	Recombinant human erythropoietin (EPO)	130:167	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	4	50	theme	hydrogen	612:619	arg1	HDX-MS					659:664	HDX-MS	659:664	HDX-MS	659:664	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	50	theme	hydrogen	612:619	arg1	spectrometry					645:656	amide hydrogen deuterium exchange mass spectrometry	606:656	amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	606:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	3	51	gly	sialylation	439:449	arg1	EPO					454:456	EPO conformation	454:469	EPO conformation	454:469	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	3	52	from	effects	428:434	arg1	conformation					458:469	EPO conformation	454:469	EPO conformation	454:469	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	3	52	from	effects	428:434	arg1	dynamics					475:482	dynamics	475:482	dynamics	475:482	Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking.					
29587225	1	53	theme	human	142:146	arg1	glycoprotein					186:197	a therapeutic glycoprotein	172:197	a therapeutic glycoprotein widely used for treating anemia	172:229	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	53	theme	human	142:146	arg1	EPO					164:166	EPO	164:166	EPO	164:166	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	1	53	theme	human	142:146	arg1	erythropoietin					148:161	Recombinant human erythropoietin	130:161	Recombinant human erythropoietin (EPO)	130:167	Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia.					
29587225	2	54	theme	receptor	325:332	arg1	binding					334:340	receptor binding	325:340	receptor binding	325:340	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	0	55	theme	recombinant	67:77	arg1	erythropoietin					85:98	recombinant human erythropoietin	67:98	recombinant human erythropoietin	67:98	Hydrogen deuterium exchange reveals changes to protein dynamics of recombinant human erythropoietin upon N- and O- desialylation.					
29587225	2	56	theme	extensive	250:258	arg1	sialylation					260:270	extensive sialylation	250:270	extensive sialylation	250:270	EPO glycans carry extensive sialylation and the level of the modification is known to affect receptor binding, protein stability and pharmacokinetics.					
29587225	4	57	theme	exchange	631:638	arg1	HDX-MS					659:664	HDX-MS	659:664	HDX-MS	659:664	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	57	theme	exchange	631:638	arg1	spectrometry					645:656	amide hydrogen deuterium exchange mass spectrometry	606:656	amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	606:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	58	theme	deuterium	621:629	arg1	HDX-MS					659:664	HDX-MS	659:664	HDX-MS	659:664	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29587225	4	58	theme	deuterium	621:629	arg1	spectrometry					645:656	amide hydrogen deuterium exchange mass spectrometry	606:656	amide hydrogen deuterium exchange mass spectrometry (HDX-MS)	606:665	Here we investigate the changes to EPO dynamics following enzymatic trimming of terminal sialic acid by amide hydrogen deuterium exchange mass spectrometry (HDX-MS).					
29231704	11	0	theme	global	1631:1636	arg1	analysis					1667:1674	global, site-specific glycosylation analysis	1631:1674	global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples	1631:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	5	1	theme	high-performance	805:820	arg1	UHPLC					845:849	UHPLC	845:849	UHPLC	845:849	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	5	1	theme	high-performance	805:820	arg1	chromatography					829:842	reversed-phase ultra high-performance liquid chromatography	784:842	reversed-phase ultra high-performance liquid chromatography (UHPLC)	784:850	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	8	2	theme	N-glycopeptides	1161:1175	arg1	total					1134:1138	A total	1132:1138	A total of 268 site-specific N-glycopeptides	1132:1175	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	4	3	theme	solid-phase	721:731	arg1	extraction					733:742	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	2	4	theme	capillary	384:392	arg1	electrophoresis					399:413	capillary zone electrophoresis	384:413	capillary zone electrophoresis separation of glycopeptides	384:441	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	10	5	gly	N-glycopeptides	1459:1473	arg2	N-glycopeptides					1459:1473	∼35% more N-glycopeptides	1449:1473	∼35% more N-glycopeptides	1449:1473	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	2	6	from	sensitive	459:467	arg1	contrast					374:381	contrast	374:381	contrast	374:381	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	1	7	theme	Reversed-phase	262:275	arg1	separation					293:302	Reversed-phase chromatographic separation	262:302	Reversed-phase chromatographic separation of glycopeptides	262:319	Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition.					
29231704	5	8	theme	reversed-phase	784:797	arg1	UHPLC					845:849	UHPLC	845:849	UHPLC	845:849	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	5	8	theme	reversed-phase	784:797	arg1	chromatography					829:842	reversed-phase ultra high-performance liquid chromatography	784:842	reversed-phase ultra high-performance liquid chromatography (UHPLC)	784:850	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	6	9	theme	mass	987:990	arg1	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	9	theme	mass	987:990	arg1	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	8	10	theme	different	1211:1219	arg1	sites					1235:1239	eight different glycosylation sites	1205:1239	eight different glycosylation sites	1205:1239	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	2	11	theme	electrophoresis	399:413	arg1	separation					415:424	capillary zone electrophoresis separation	384:424	capillary zone electrophoresis separation of glycopeptides	384:441	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	1	12	theme	glycopeptides	307:319	arg1	separation					293:302	Reversed-phase chromatographic separation	262:302	Reversed-phase chromatographic separation of glycopeptides	262:319	Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition.					
29231704	10	13	gly	N-glycopeptides	1541:1555	arg2	N-glycopeptides					1541:1555	∼70% more N-glycopeptides	1531:1555	∼70% more N-glycopeptides	1531:1555	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	0	14	theme	Capillary	181:189	arg1	Spectrometry					248:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	6	15	used	used	1025:1028	arg2	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	15	used	used	1025:1028	arg2	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	8	16	gly	glycosylation	1221:1233	arg2	sites					1235:1239	eight different glycosylation sites	1205:1239	eight different glycosylation sites	1205:1239	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	8	16	gly	glycosylation	1221:1233	arg2	eight					1205:1209	eight	1205:1209	eight	1205:1209	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	0	17	theme	Electrophoresis-Electrospray	196:223	arg1	Spectrometry					248:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	9	18	with	acids	1296:1300	arg1	repeats					1342:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	11	19	with	glycoproteins	1700:1712	arg1	samples					1732:1738	mass-limited samples	1719:1738	mass-limited samples	1719:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	7	20	gly	glycoprotein	1112:1123	arg1	AGP					1126:1128	AGP	1126:1128	AGP	1126:1128	We applied this method for the analysis of alpha-1-acid glycoprotein (AGP).					
29231704	7	20	gly	glycoprotein	1112:1123	arg1	glycoprotein					1112:1123	alpha-1-acid glycoprotein	1099:1123	alpha-1-acid glycoprotein (AGP)	1099:1129	We applied this method for the analysis of alpha-1-acid glycoprotein (AGP).					
29231704	0	21	theme	Mass	243:246	arg1	Spectrometry					248:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	10	22	theme	direct	1562:1567	arg1	analysis					1583:1590	direct CZE-ESI-MS/MS analysis	1562:1590	direct CZE-ESI-MS/MS analysis	1562:1590	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	0	23	theme	Chromatography	86:99	arg1	Extraction					113:122	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	10	24	theme	UHPLC-ESI-MS/MS	1502:1516	arg1	analysis					1518:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	10	25	theme	direct	1480:1485	arg1	analysis					1518:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	0	26	theme	Reversed-Phase	125:138	arg1	Chromatography					147:160	Reversed-Phase Liquid Chromatography	125:160	Reversed-Phase Liquid Chromatography Fractionation	125:174	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	9	27	theme	tetra-sialic	1283:1294	arg1	acids					1296:1300	tetra-sialic acids	1283:1300	tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats	1283:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	0	28	theme	Chromatography	147:160	arg1	Fractionation					162:174	Reversed-Phase Liquid Chromatography Fractionation	125:174	Reversed-Phase Liquid Chromatography Fractionation	125:174	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	11	29	theme	glycoproteins	1700:1712	arg1	analysis					1667:1674	global, site-specific glycosylation analysis	1631:1674	global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples	1631:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	0	30	theme	Site-Specific	0:12	arg1	Characterization					35:50	Site-Specific Glycan Heterogeneity Characterization	0:50	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.	0:260	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	4	31	theme	hydrophilic	667:677	arg1	HILIC					714:718	HILIC	714:718	HILIC	714:718	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	4	31	theme	hydrophilic	667:677	arg1	chromatography					698:711	hydrophilic interaction liquid chromatography	667:711	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	9	32	theme	N-acetyllactosamine	1313:1331	arg1	repeats					1342:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	0	33	theme	Heterogeneity	21:33	arg1	Characterization					35:50	Site-Specific Glycan Heterogeneity Characterization	0:50	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.	0:260	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	4	34	theme	liquid	691:696	arg1	HILIC					714:718	HILIC	714:718	HILIC	714:718	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	4	34	theme	liquid	691:696	arg1	chromatography					698:711	hydrophilic interaction liquid chromatography	667:711	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	9	35	theme	LacNAc	1334:1339	arg1	repeats					1342:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	6	36	theme	zone	935:938	arg1	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	36	theme	zone	935:938	arg1	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	0	37	theme	Interaction	67:77	arg1	Chromatography					86:99	Hydrophilic Interaction Liquid Chromatography	55:99	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	9	38	theme	unusual	1354:1360	arg1	acids					1394:1398	unusual pentasialylated terminal sialic acids	1354:1398	unusual pentasialylated terminal sialic acids	1354:1398	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	3	39	theme	N-glycopeptide	580:593	arg1	analysis					595:602	superior N-glycopeptide analysis	571:602	superior N-glycopeptide analysis	571:602	In this paper, we combine the two techniques to achieve superior N-glycopeptide analysis.					
29231704	9	40	theme	terminal	1378:1385	arg1	acids					1394:1398	unusual pentasialylated terminal sialic acids	1354:1398	unusual pentasialylated terminal sialic acids	1354:1398	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	7	41	theme	alpha-1-acid	1099:1110	arg1	AGP					1126:1128	AGP	1126:1128	AGP	1126:1128	We applied this method for the analysis of alpha-1-acid glycoprotein (AGP).					
29231704	7	41	theme	alpha-1-acid	1099:1110	arg1	glycoprotein					1112:1123	alpha-1-acid glycoprotein	1099:1123	alpha-1-acid glycoprotein (AGP)	1099:1129	We applied this method for the analysis of alpha-1-acid glycoprotein (AGP).					
29231704	10	42	theme	Reversed-phase	1401:1414	arg1	UHPLC					1416:1420	Reversed-phase UHPLC	1401:1420	Reversed-phase UHPLC coupled with CZE	1401:1437	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	8	43	theme	site-specific	1147:1159	arg1	N-glycopeptides					1161:1175	268 site-specific N-glycopeptides	1143:1175	268 site-specific N-glycopeptides	1143:1175	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	5	44	theme	ultra	799:803	arg1	UHPLC					845:849	UHPLC	845:849	UHPLC	845:849	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	5	44	theme	ultra	799:803	arg1	chromatography					829:842	reversed-phase ultra high-performance liquid chromatography	784:842	reversed-phase ultra high-performance liquid chromatography (UHPLC)	784:850	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	2	45	theme	sialic	476:481	arg1	composition					488:498	the sialic acid composition	472:498	the sialic acid composition of the glycan	472:512	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	1	46	theme	peptide	350:356	arg1	composition					358:368	the peptide composition	346:368	the peptide composition	346:368	Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition.					
29231704	5	47	theme	liquid	822:827	arg1	UHPLC					845:849	UHPLC	845:849	UHPLC	845:849	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	5	47	theme	liquid	822:827	arg1	chromatography					829:842	reversed-phase ultra high-performance liquid chromatography	784:842	reversed-phase ultra high-performance liquid chromatography (UHPLC)	784:850	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	2	48	theme	zone	394:397	arg1	electrophoresis					399:413	capillary zone electrophoresis	384:413	capillary zone electrophoresis separation of glycopeptides	384:441	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	9	49	gly	pentasialylated	1362:1376	arg1	acids					1394:1398	unusual pentasialylated terminal sialic acids	1354:1398	unusual pentasialylated terminal sialic acids	1354:1398	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	11	50	theme	glycosylation	1653:1665	arg1	analysis					1667:1674	global, site-specific glycosylation analysis	1631:1674	global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples	1631:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	1	51	gly	glycopeptides	307:319	arg2	glycopeptides					307:319	glycopeptides	307:319	glycopeptides	307:319	Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition.					
29231704	8	52	gly	N-glycopeptides	1161:1175	arg2	N-glycopeptides					1161:1175	268 site-specific N-glycopeptides	1143:1175	268 site-specific N-glycopeptides	1143:1175	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	11	53	gly	glycoproteins	1700:1712	arg1	glycoproteins					1700:1712	highly heterogeneous glycoproteins	1679:1712	highly heterogeneous glycoproteins with mass-limited samples	1679:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	2	54	theme	glycopeptides	429:441	arg1	separation					415:424	capillary zone electrophoresis separation	384:424	capillary zone electrophoresis separation of glycopeptides	384:441	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	1	55	theme	chromatographic	277:291	arg1	separation					293:302	Reversed-phase chromatographic separation	262:302	Reversed-phase chromatographic separation of glycopeptides	262:319	Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition.					
29231704	8	56	theme	glycosylation	1221:1233	arg1	sites					1235:1239	eight different glycosylation sites	1205:1239	eight different glycosylation sites	1205:1239	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	6	57	theme	ionization-tandem	969:985	arg1	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	57	theme	ionization-tandem	969:985	arg1	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	11	58	dep	global	1631:1636	arg1	site-specific					1639:1651	site-specific	1639:1651	site-specific	1639:1651	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	11	59	theme	promising	1612:1620	arg1	approach					1598:1605	This approach	1593:1605	This approach	1593:1605	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	11	59	theme	promising	1612:1620	arg1	tool					1622:1625	a promising tool	1610:1625	a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples	1610:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	0	60	theme	Zone	191:194	arg1	Spectrometry					248:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	0	61	theme	Ionization-Tandem	225:241	arg1	Spectrometry					248:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry	181:259	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	2	62	from	contrast	374:381	arg1	sensitive					459:467	sensitive	459:467	sensitive	459:467	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	10	63	theme	more	1454:1457	arg1	N-glycopeptides					1459:1473	∼35% more N-glycopeptides	1449:1473	∼35% more N-glycopeptides	1449:1473	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	4	64	attach	isolated	630:637	arg1	digest					654:659	a tryptic digest	644:659	a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	644:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	4	64	attach	isolated	630:637	arg2	Glycopeptides					605:617	Glycopeptides	605:617	Glycopeptides	605:617	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	0	65	theme	Solid-Phase	101:111	arg1	Extraction					113:122	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	5	66	theme	different	896:904	arg1	backbones					914:922	different peptide backbones	896:922	different peptide backbones	896:922	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	10	67	theme	reversed-phase	1487:1500	arg1	analysis					1518:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	direct reversed-phase UHPLC-ESI-MS/MS analysis	1480:1525	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	0	68	theme	Liquid	140:145	arg1	Chromatography					147:160	Reversed-Phase Liquid Chromatography	125:160	Reversed-Phase Liquid Chromatography Fractionation	125:174	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	10	69	theme	more	1536:1539	arg1	N-glycopeptides					1541:1555	∼70% more N-glycopeptides	1531:1555	∼70% more N-glycopeptides	1531:1555	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	5	70	theme	peptide	906:912	arg1	backbones					914:922	different peptide backbones	896:922	different peptide backbones	896:922	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	8	71	theme	AGP	1261:1263	arg1	isomers					1250:1256	two isomers	1246:1256	two isomers of AGP	1246:1263	A total of 268 site-specific N-glycopeptides were detected, representing eight different glycosylation sites from two isomers of AGP.					
29231704	11	72	gly	glycosylation	1653:1665	arg1	glycoproteins					1700:1712	highly heterogeneous glycoproteins	1679:1712	highly heterogeneous glycoproteins with mass-limited samples	1679:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	11	73	theme	heterogeneous	1686:1698	arg1	glycoproteins					1700:1712	highly heterogeneous glycoproteins	1679:1712	highly heterogeneous glycoproteins with mass-limited samples	1679:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	2	74	theme	glycan	507:512	arg1	composition					488:498	the sialic acid composition	472:498	the sialic acid composition of the glycan	472:512	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	0	75	theme	Glycan	14:19	arg1	Characterization					35:50	Site-Specific Glycan Heterogeneity Characterization	0:50	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.	0:260	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	4	76	theme	interaction	679:689	arg1	HILIC					714:718	HILIC	714:718	HILIC	714:718	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	4	76	theme	interaction	679:689	arg1	chromatography					698:711	hydrophilic interaction liquid chromatography	667:711	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	10	77	theme	CZE-ESI-MS/MS	1569:1581	arg1	analysis					1583:1590	direct CZE-ESI-MS/MS analysis	1562:1590	direct CZE-ESI-MS/MS analysis	1562:1590	Reversed-phase UHPLC coupled with CZE generated ∼35% more N-glycopeptides than direct reversed-phase UHPLC-ESI-MS/MS analysis and ∼70% more N-glycopeptides than direct CZE-ESI-MS/MS analysis.					
29231704	4	78	theme	chromatography	698:711	arg1	extraction					733:742	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	667:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	3	79	gly	N-glycopeptide	580:593	arg2	N-glycopeptide					580:593	superior N-glycopeptide analysis	571:602	superior N-glycopeptide analysis	571:602	In this paper, we combine the two techniques to achieve superior N-glycopeptide analysis.					
29231704	3	80	theme	superior	571:578	arg1	analysis					595:602	superior N-glycopeptide analysis	571:602	superior N-glycopeptide analysis	571:602	In this paper, we combine the two techniques to achieve superior N-glycopeptide analysis.					
29231704	0	81	theme	Hydrophilic	55:65	arg1	Chromatography					86:99	Hydrophilic Interaction Liquid Chromatography	55:99	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	11	82	theme	mass-limited	1719:1730	arg1	samples					1732:1738	mass-limited samples	1719:1738	mass-limited samples	1719:1738	This approach is a promising tool for global, site-specific glycosylation analysis of highly heterogeneous glycoproteins with mass-limited samples.					
29231704	4	83	theme	tryptic	646:652	arg1	digest					654:659	a tryptic digest	644:659	a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction	644:742	Glycopeptides were first isolated from a tryptic digest using hydrophilic interaction liquid chromatography (HILIC) solid-phase extraction.					
29231704	9	84	theme	multi	1307:1311	arg1	repeats					1342:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	6	85	theme	electrophoresis-electrospray	940:967	arg1	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	85	theme	electrophoresis-electrospray	940:967	arg1	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	0	86	theme	Liquid	79:84	arg1	Chromatography					86:99	Hydrophilic Interaction Liquid Chromatography	55:99	Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction	55:122	Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry.					
29231704	6	87	theme	Capillary	925:933	arg1	spectrometry					992:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry	925:1003	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS)	925:1019	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	6	87	theme	Capillary	925:933	arg1	CZE-ESI-MS/MS					1006:1018	CZE-ESI-MS/MS	1006:1018	CZE-ESI-MS/MS	1006:1018	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) was used to analyze the fractions.					
29231704	7	88	theme	glycoprotein	1112:1123	arg1	analysis					1087:1094	the analysis	1083:1094	the analysis of alpha-1-acid glycoprotein (AGP)	1083:1129	We applied this method for the analysis of alpha-1-acid glycoprotein (AGP).					
29231704	9	89	theme	pentasialylated	1362:1376	arg1	acids					1394:1398	unusual pentasialylated terminal sialic acids	1354:1398	unusual pentasialylated terminal sialic acids	1354:1398	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	9	90	with	acids	1394:1398	arg1	repeats					1342:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	multi N-acetyllactosamine (LacNAc) repeats	1307:1348	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	5	91	gly	glycopeptides	749:761	arg2	glycopeptides					749:761	The glycopeptides	745:761	The glycopeptides	745:761	The glycopeptides were separated using reversed-phase ultra high-performance liquid chromatography (UHPLC) to generate four fractions corresponding to different peptide backbones.					
29231704	2	92	gly	glycopeptides	429:441	arg2	glycopeptides					429:441	glycopeptides	429:441	glycopeptides	429:441	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29231704	9	93	theme	sialic	1387:1392	arg1	acids					1394:1398	unusual pentasialylated terminal sialic acids	1354:1398	unusual pentasialylated terminal sialic acids	1354:1398	Glycans included tetra-sialic acids with multi N-acetyllactosamine (LacNAc) repeats and unusual pentasialylated terminal sialic acids.					
29231704	2	94	theme	acid	483:486	arg1	composition					488:498	the sialic acid composition	472:498	the sialic acid composition of the glycan	472:512	In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan.					
29580922	8	0	theme	haptoglobin	1375:1385	arg1	N-glycans					1362:1370	tri-antennary and sialylated N-glycans	1333:1370	tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites	1333:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	6	1	theme	clinical	1000:1007	arg1	biomarkers					1009:1018	clinical biomarkers	1000:1018	clinical biomarkers	1000:1018	Furthermore, plasma levels of clinical biomarkers were measured.					
29580922	7	2	theme	protein	1135:1141	arg1	binding					1143:1149	protein binding	1135:1149	protein binding	1135:1149	RESULTS: We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs.					
29580922	9	3	theme	Sialylated	1442:1451	arg1	glycans					1453:1459	Sialylated glycans	1442:1459	Sialylated glycans of haptoglobin	1442:1474	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	3	theme	Sialylated	1442:1451	arg1	biomarker					1492:1500	a potential biomarker	1480:1500	a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis	1480:1590	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	1	4	theme	physiological	178:190	arg1	conditions					192:201	the physiological conditions	174:201	the physiological conditions	174:201	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.					
29580922	9	5	theme	liver	1557:1561	arg1	cirrhosis					1563:1571	liver cirrhosis	1557:1571	liver cirrhosis	1557:1571	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	6	theme	diseases	1513:1520	arg1	glycans					1453:1459	Sialylated glycans	1442:1459	Sialylated glycans of haptoglobin	1442:1474	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	6	theme	diseases	1513:1520	arg1	biomarker					1492:1500	a potential biomarker	1480:1500	a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis	1480:1590	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	1	7	theme	conditions	192:201	arg1	changes					163:169	changes	163:169	changes of the physiological conditions	163:201	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.					
29580922	11	8	theme	human	1811:1815	arg1	longevity					1817:1825	extreme human longevity	1803:1825	extreme human longevity	1803:1825	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	8	9	gly	sialylated	1351:1360	arg1	N-glycans					1362:1370	tri-antennary and sialylated N-glycans	1333:1370	tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites	1333:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	11	10	from	sites	1753:1757	arg1	haptoglobin					1716:1726	haptoglobin	1716:1726	haptoglobin at the Asn207 and Asn211 sites	1716:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	5	11	dep	structures	926:935	arg1	OPLS-DA					960:966	OPLS-DA	960:966	OPLS-DA	960:966	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	5	11	dep	structures	926:935	arg1	analysis					950:957	discriminant analysis	937:957	latent structures discriminant analysis (OPLS-DA)	919:967	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	11	12	theme	longevity	1817:1825	arg1	CONCLUSIONS					1661:1671	CONCLUSIONS	1661:1671	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.	1661:1826	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	11	12	theme	longevity	1817:1825	arg1	characteristic					1785:1798	characteristic	1785:1798	characteristic	1785:1798	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	6	13	theme	biomarkers	1009:1018	arg1	levels					990:995	plasma levels	983:995	plasma levels of clinical biomarkers	983:1018	Furthermore, plasma levels of clinical biomarkers were measured.					
29580922	8	14	theme	Asn207	1390:1395	arg1	sites					1408:1412	Asn207 and Asn211 sites	1390:1412	Asn207 and Asn211 sites	1390:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	5	15	theme	discriminant	937:948	arg1	OPLS-DA					960:966	OPLS-DA	960:966	OPLS-DA	960:966	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	5	15	theme	discriminant	937:948	arg1	analysis					950:957	discriminant analysis	937:957	latent structures discriminant analysis (OPLS-DA)	919:967	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	4	16	from	controls	685:692	arg1	proteins					577:584	Plasma proteins	570:584	Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years)	570:706	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	9	17	theme	potential	1482:1490	arg1	glycans					1453:1459	Sialylated glycans	1442:1459	Sialylated glycans of haptoglobin	1442:1474	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	17	theme	potential	1482:1490	arg1	biomarker					1492:1500	a potential biomarker	1480:1500	a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis	1480:1590	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	8	18	theme	area	1199:1202	arg1	ratios					1204:1209	Peak area ratios	1194:1209	Peak area ratios of ECA-enriched glycopeptides	1194:1239	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	11	19	theme	Asn207	1735:1740	arg1	sites					1753:1757	the Asn207 and Asn211 sites	1731:1757	the Asn207 and Asn211 sites	1731:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	8	20	gly	glycopeptides	1227:1239	arg2	glycopeptides					1227:1239	ECA-enriched glycopeptides	1214:1239	ECA-enriched glycopeptides	1214:1239	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	3	21	theme	longevity	550:558	arg1	characteristic					518:531	characteristic	518:531	characteristic	518:531	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	8	22	theme	ECA-enriched	1214:1225	arg1	glycopeptides					1227:1239	ECA-enriched glycopeptides	1214:1239	ECA-enriched glycopeptides	1214:1239	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	3	23	theme	SSCs	454:457	arg1	samples					443:449	plasma samples	436:449	plasma samples of SSCs	436:457	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	4	24	from	semi-supercentenarians	600:621	arg1	proteins					577:584	Plasma proteins	570:584	Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years)	570:706	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	9	25	theme	haptoglobin	1464:1474	arg1	glycans					1453:1459	Sialylated glycans	1442:1459	Sialylated glycans of haptoglobin	1442:1474	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	25	theme	haptoglobin	1464:1474	arg1	biomarker					1492:1500	a potential biomarker	1480:1500	a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis	1480:1590	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	5	26	theme	glycopeptides	823:835	arg1	ratios					813:818	Peak area ratios	803:818	Peak area ratios of glycopeptides to corresponding normalising peptides	803:873	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	12	27	theme	GENERAL	1828:1834	arg1	SIGNIFICANCE					1836:1847	GENERAL SIGNIFICANCE	1828:1847	GENERAL SIGNIFICANCE: We found abundant glycans in SSCs, which may be associated with human longevity.	1828:1929	GENERAL SIGNIFICANCE: We found abundant glycans in SSCs, which may be associated with human longevity.					
29580922	5	28	theme	orthogonal	893:902	arg1	projections					904:914	orthogonal projections	893:914	orthogonal projections to latent structures discriminant analysis (OPLS-DA)	893:967	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	8	29	theme	sialylated	1351:1360	arg1	N-glycans					1362:1370	tri-antennary and sialylated N-glycans	1333:1370	tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites	1333:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	11	30	from	abundant	1764:1771	arg1	SSCs					1776:1779	SSCs	1776:1779	SSCs	1776:1779	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	9	31	theme	several	1505:1511	arg1	cirrhosis					1563:1571	liver cirrhosis	1557:1571	liver cirrhosis	1557:1571	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	31	theme	several	1505:1511	arg1	carcinoma					1546:1554	hepatocellular carcinoma	1531:1554	hepatocellular carcinoma	1531:1554	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	31	theme	several	1505:1511	arg1	diseases					1513:1520	several diseases	1505:1520	several diseases	1505:1520	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	9	31	theme	several	1505:1511	arg1	IgA-nephritis					1578:1590	IgA-nephritis	1578:1590	IgA-nephritis	1578:1590	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	0	32	theme	Characteristic	0:13	arg1	glycopeptides					15:27	Characteristic glycopeptides	0:27	Characteristic glycopeptides associated with extreme human longevity	0:67	Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.					
29580922	12	33	dep	SIGNIFICANCE	1836:1847	arg1	found					1853:1857	found	1853:1857	found abundant glycans in SSCs, which may be associated with human longevity	1853:1928	GENERAL SIGNIFICANCE: We found abundant glycans in SSCs, which may be associated with human longevity.					
29580922	4	34	theme	young	679:683	arg1	20-38 years					695:705	20-38 years	695:705	20-38 years	695:705	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	4	34	theme	young	679:683	arg1	controls					685:692	young controls	679:692	young controls (20-38 years)	679:706	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	8	35	theme	glycopeptides	1227:1239	arg1	ratios					1204:1209	Peak area ratios	1194:1209	Peak area ratios of ECA-enriched glycopeptides	1194:1239	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	4	36	theme	Plasma	570:575	arg1	proteins					577:584	Plasma proteins	570:584	Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years)	570:706	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	7	37	theme	Erythrina	1097:1105	arg1	ECA					1120:1122	ECA	1120:1122	ECA	1120:1122	RESULTS: We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs.					
29580922	7	37	theme	Erythrina	1097:1105	arg1	cristagalli					1107:1117	Erythrina cristagalli	1097:1117	Erythrina cristagalli (ECA)	1097:1123	RESULTS: We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs.					
29580922	8	38	from	N-glycans	1362:1370	arg1	sites					1408:1412	Asn207 and Asn211 sites	1390:1412	Asn207 and Asn211 sites	1390:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	4	39	theme	chromatography/mass	761:779	arg1	LC/MS					795:799	LC/MS	795:799	LC/MS	795:799	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	4	39	theme	chromatography/mass	761:779	arg1	spectrometry					781:792	liquid chromatography/mass spectrometry	754:792	liquid chromatography/mass spectrometry (LC/MS)	754:800	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	5	40	theme	normalising	854:864	arg1	peptides					866:873	corresponding normalising peptides	840:873	corresponding normalising peptides	840:873	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	5	41	theme	corresponding	840:852	arg1	peptides					866:873	corresponding normalising peptides	840:873	corresponding normalising peptides	840:873	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	4	42	theme	Japanese	591:598	arg1	semi-supercentenarians					600:621	Japanese semi-supercentenarians	591:621	Japanese semi-supercentenarians (SSCs, 106-109 years)	591:643	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	2	43	theme	longevity	384:392	arg1	model					369:373	a model	367:373	a model of human longevity	367:392	Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity.					
29580922	2	43	theme	longevity	384:392	arg1	Semi-supercentenarians					297:318	Semi-supercentenarians	297:318	Semi-supercentenarians (SSCs; older than 105 years)	297:347	Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity.					
29580922	4	44	theme	liquid	754:759	arg1	LC/MS					795:799	LC/MS	795:799	LC/MS	795:799	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	4	44	theme	liquid	754:759	arg1	spectrometry					781:792	liquid chromatography/mass spectrometry	754:792	liquid chromatography/mass spectrometry (LC/MS)	754:800	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	3	45	theme	extreme	536:542	arg1	longevity					550:558	extreme human longevity	536:558	extreme human longevity	536:558	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	0	46	theme	human	53:57	arg1	longevity					59:67	extreme human longevity	45:67	extreme human longevity	45:67	Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.					
29580922	11	47	gly	sialylated	1692:1701	arg1	N-glycans					1703:1711	Tri-antennary and sialylated N-glycans	1674:1711	Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites	1674:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	5	48	gly	glycopeptides	823:835	arg2	glycopeptides					823:835	glycopeptides	823:835	glycopeptides	823:835	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	4	49	from	controls	651:658	arg1	proteins					577:584	Plasma proteins	570:584	Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years)	570:706	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	1	50	theme	potential	226:234	arg1	BACKGROUND					112:121	BACKGROUND	112:121	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.	112:295	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.					
29580922	1	50	theme	potential	226:234	arg1	biomarker					236:244	a potential biomarker	224:244	a potential biomarker associated with several diseases and/or longevity	224:294	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.					
29580922	0	51	theme	extreme	45:51	arg1	longevity					59:67	extreme human longevity	45:67	extreme human longevity	45:67	Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.					
29580922	3	52	theme	plasma	436:441	arg1	samples					443:449	plasma samples	436:449	plasma samples of SSCs	436:457	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	11	53	from	SSCs	1776:1779	arg1	CONCLUSIONS					1661:1671	CONCLUSIONS	1661:1671	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.	1661:1826	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	11	53	from	SSCs	1776:1779	arg1	abundant					1764:1771	abundant	1764:1771	abundant	1764:1771	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	0	54	gly	glycopeptides	15:27	arg2	glycopeptides					15:27	Characteristic glycopeptides	0:27	Characteristic glycopeptides associated with extreme human longevity	0:67	Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.					
29580922	8	55	theme	Peak	1194:1197	arg1	ratios					1204:1209	Peak area ratios	1194:1209	Peak area ratios of ECA-enriched glycopeptides	1194:1239	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	5	56	theme	area	808:811	arg1	ratios					813:818	Peak area ratios	803:818	Peak area ratios of glycopeptides to corresponding normalising peptides	803:873	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	5	57	theme	latent	919:924	arg1	structures					926:935	latent structures	919:935	latent structures discriminant analysis (OPLS-DA)	919:967	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	7	58	theme	Phaseolus	1073:1081	arg1	vulgaris					1083:1090	Phaseolus vulgaris	1073:1090	Phaseolus vulgaris	1073:1090	RESULTS: We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs.					
29580922	4	59	dep	semi-supercentenarians	600:621	arg1	SSCs					624:627	SSCs	624:627	SSCs	624:627	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	4	59	dep	semi-supercentenarians	600:621	arg1	106-109 years					630:642	106-109 years	630:642	106-109 years	630:642	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	8	60	theme	tri-antennary	1333:1345	arg1	N-glycans					1362:1370	tri-antennary and sialylated N-glycans	1333:1370	tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites	1333:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	11	61	theme	sialylated	1692:1701	arg1	N-glycans					1703:1711	Tri-antennary and sialylated N-glycans	1674:1711	Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites	1674:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	12	62	theme	human	1914:1918	arg1	longevity					1920:1928	human longevity	1914:1928	human longevity	1914:1928	GENERAL SIGNIFICANCE: We found abundant glycans in SSCs, which may be associated with human longevity.					
29580922	8	63	from	sites	1408:1412	arg1	haptoglobin					1375:1385	haptoglobin	1375:1385	haptoglobin at Asn207 and Asn211 sites	1375:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	8	63	from	sites	1408:1412	arg1	N-glycans					1362:1370	tri-antennary and sialylated N-glycans	1333:1370	tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites	1333:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	11	64	theme	Tri-antennary	1674:1686	arg1	N-glycans					1703:1711	Tri-antennary and sialylated N-glycans	1674:1711	Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites	1674:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	3	65	theme	conjugated	488:497	arg1	N-glycans					499:507	conjugated N-glycans	488:507	conjugated N-glycans	488:507	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	11	66	theme	extreme	1803:1809	arg1	longevity					1817:1825	extreme human longevity	1803:1825	extreme human longevity	1803:1825	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	8	67	theme	Asn211	1401:1406	arg1	sites					1408:1412	Asn207 and Asn211 sites	1390:1412	Asn207 and Asn211 sites	1390:1412	Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs.					
29580922	3	68	theme	human	544:548	arg1	longevity					550:558	extreme human longevity	536:558	extreme human longevity	536:558	Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.					
29580922	4	69	theme	lectin	731:736	arg1	microarrays					738:748	lectin microarrays	731:748	lectin microarrays	731:748	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	0	70	theme	plasma	88:93	arg1	glycoproteomics					95:109	plasma glycoproteomics	88:109	plasma glycoproteomics	88:109	Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.					
29580922	9	71	theme	hepatocellular	1531:1544	arg1	carcinoma					1546:1554	hepatocellular carcinoma	1531:1554	hepatocellular carcinoma	1531:1554	Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis.					
29580922	4	72	theme	aged	646:649	arg1	70-88 years					661:671	70-88 years	661:671	70-88 years	661:671	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	4	72	theme	aged	646:649	arg1	controls					651:658	aged controls	646:658	aged controls (70-88 years)	646:672	METHODS: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS).					
29580922	2	73	dep	SSCs	321:324	arg1	older					327:331	older	327:331	older	327:331	Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity.					
29580922	1	74	theme	several	262:268	arg1	diseases					270:277	several diseases	262:277	several diseases	262:277	BACKGROUND: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity.					
29580922	11	75	from	N-glycans	1703:1711	arg1	haptoglobin					1716:1726	haptoglobin	1716:1726	haptoglobin at the Asn207 and Asn211 sites	1716:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	5	76	theme	Peak	803:806	arg1	ratios					813:818	Peak area ratios	803:818	Peak area ratios of glycopeptides to corresponding normalising peptides	803:873	Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA).					
29580922	12	77	theme	abundant	1859:1866	arg1	glycans					1868:1874	abundant glycans	1859:1874	abundant glycans	1859:1874	GENERAL SIGNIFICANCE: We found abundant glycans in SSCs, which may be associated with human longevity.					
29580922	2	78	theme	human	378:382	arg1	longevity					384:392	human longevity	378:392	human longevity	378:392	Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity.					
29580922	11	79	theme	Asn211	1746:1751	arg1	sites					1753:1757	the Asn207 and Asn211 sites	1731:1757	the Asn207 and Asn211 sites	1731:1757	CONCLUSIONS: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.					
29580922	6	80	theme	plasma	983:988	arg1	levels					990:995	plasma levels	983:995	plasma levels of clinical biomarkers	983:1018	Furthermore, plasma levels of clinical biomarkers were measured.					
29703890	0	0	theme	large-scale	83:93	arg1	analysis					95:102	large-scale analysis	83:102	large-scale analysis of glycoproteins	83:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	5	1	theme	several	693:699	arg1	derivatives					714:724	several boronic acid derivatives	693:724	several boronic acid derivatives	693:724	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	7	2	gly	glycopeptides	1086:1098	arg2	glycopeptides					1086:1098	low-abundance glycopeptides	1072:1098	low-abundance glycopeptides	1072:1098	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	5	3	theme	boronic	701:707	arg1	derivatives					714:724	several boronic acid derivatives	693:724	several boronic acid derivatives	693:724	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	1	4	from	systems	172:178	arg1	ubiquitous					147:156	ubiquitous	147:156	ubiquitous	147:156	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	3	5	theme	low-abundance	534:546	arg1	species					548:554	low-abundance species	534:554	low-abundance species	534:554	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	7	6	theme	robust	966:971	arg1	method					984:989	This robust and simple method	961:989	This robust and simple method	961:989	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	8	7	theme	enriched	1118:1125	arg1	glycopeptides					1127:1139	the enriched glycopeptides	1114:1139	the enriched glycopeptides	1114:1139	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	5	8	theme	acid	709:712	arg1	derivatives					714:724	several boronic acid derivatives	693:724	several boronic acid derivatives	693:724	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	3	9	theme	great	426:430	arg1	potential					432:440	great potential	426:440	great potential	426:440	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	3	10	theme	boronic	396:402	arg1	acid					404:407	boronic acid	396:407	boronic acid	396:407	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	5	11	theme	derivatives	714:724	arg1	performance					678:688	the performance	674:688	the performance of several boronic acid derivatives	674:724	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	3	12	theme	covalent	366:373	arg1	interactions					375:386	reversible covalent interactions	355:386	reversible covalent interactions between boronic acid and glycans	355:419	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	2	13	theme	glycoproteins	281:293	arg1	heterogeneity					226:238	the heterogeneity	222:238	the heterogeneity of glycans	222:249	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	2	13	theme	glycoproteins	281:293	arg1	abundance					263:271	the low abundance	255:271	the low abundance of many glycoproteins	255:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	0	14	theme	glycoproteins	107:119	arg1	analysis					95:102	large-scale analysis	83:102	large-scale analysis of glycoproteins	83:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	6	15	theme	benzoboroxole-glycan	926:945	arg1	interactions					947:958	synergistic benzoboroxole-glycan interactions	914:958	synergistic benzoboroxole-glycan interactions	914:958	The enrichment is further improved by conjugating benzoboroxole to a dendrimer, which enables synergistic benzoboroxole-glycan interactions.					
29703890	2	16	theme	many	276:279	arg1	glycoproteins					281:293	many glycoproteins	276:293	many glycoproteins	276:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	5	17	theme	cell	806:809	arg1	lysates					811:817	human cell lysates	800:817	human cell lysates	800:817	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	0	18	theme	enrichment	3:12	arg1	method					14:19	An enrichment method	0:19	An enrichment method	0:19	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	8	19	theme	mass-spectrometry-based	1198:1220	arg1	approaches					1222:1231	mass-spectrometry-based approaches	1198:1231	mass-spectrometry-based approaches	1198:1231	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	1	20	from	ubiquitous	147:156	arg1	systems					172:178	biological systems	161:178	biological systems	161:178	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	8	21	gly	glycosylation	1245:1257	arg2	sites					1259:1263	glycosylation sites	1245:1263	glycosylation sites	1245:1263	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	1	22	theme	cell	198:201	arg1	survival					203:210	cell survival	198:210	cell survival	198:210	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	8	23	theme	glycosylation	1245:1257	arg1	sites					1259:1263	glycosylation sites	1245:1263	glycosylation sites	1245:1263	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	3	24	contain	have	421:424	arg1	methods					338:344	Chemical methods	329:344	Chemical methods based on reversible covalent interactions between boronic acid and glycans	329:419	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	3	24	contain	have	421:424	arg2	potential					432:440	great potential	426:440	great potential	426:440	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	7	25	theme	sensitive	1015:1023	arg1	analysis					1041:1048	sensitive glycoproteomics analysis	1015:1048	sensitive glycoproteomics analysis	1015:1048	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	1	26	gly	glycosylation	130:142	arg1	cell					198:201	cell survival	198:210	cell survival	198:210	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	7	27	theme	glycoproteomics	1025:1039	arg1	analysis					1041:1048	sensitive glycoproteomics analysis	1015:1048	sensitive glycoproteomics analysis	1015:1048	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	0	28	gly	glycoproteins	107:119	arg1	glycoproteins					107:119	glycoproteins	107:119	glycoproteins	107:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	4	29	gly	glycopeptide	641:652	arg2	glycopeptide					641:652	the glycopeptide enrichment	637:663	the glycopeptide enrichment	637:663	Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment.					
29703890	3	30	theme	reversible	355:364	arg1	interactions					375:386	reversible covalent interactions	355:386	reversible covalent interactions between boronic acid and glycans	355:419	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	4	31	theme	dendrimer-conjugated	591:610	arg1	benzoboroxole					612:624	dendrimer-conjugated benzoboroxole	591:624	dendrimer-conjugated benzoboroxole	591:624	Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment.					
29703890	2	32	theme	global	312:317	arg1	analysis					319:326	their global analysis	306:326	their global analysis	306:326	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	8	33	with	compatible	1182:1191	arg1	approaches					1222:1231	mass-spectrometry-based approaches	1198:1231	mass-spectrometry-based approaches	1198:1231	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	0	34	theme	synergistic	30:40	arg1	interactions					66:77	synergistic and reversible covalent interactions	30:77	synergistic and reversible covalent interactions for large-scale analysis of glycoproteins	30:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	5	35	theme	glycopeptide	773:784	arg1	coverage					786:793	glycopeptide coverage	773:793	glycopeptide coverage from human cell lysates	773:817	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	5	36	from	lysates	811:817	arg1	coverage					786:793	glycopeptide coverage	773:793	glycopeptide coverage from human cell lysates	773:817	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	3	37	theme	binding	475:481	arg1	affinity					483:490	the binding affinity	471:490	the binding affinity	471:490	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	3	37	theme	binding	475:481	arg1	strong					509:514	strong	509:514	strong	509:514	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	8	38	gly	glycopeptides	1127:1139	arg2	glycopeptides					1127:1139	the enriched glycopeptides	1114:1139	the enriched glycopeptides	1114:1139	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	8	39	dep	identify	1236:1243	arg1	compatible					1182:1191	compatible	1182:1191	compatible	1182:1191	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	2	40	theme	glycans	243:249	arg1	heterogeneity					226:238	the heterogeneity	222:238	the heterogeneity of glycans	222:249	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	2	40	theme	glycans	243:249	arg1	abundance					263:271	the low abundance	255:271	the low abundance of many glycoproteins	255:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	2	41	gly	glycoproteins	281:293	arg1	glycoproteins					281:293	many glycoproteins	276:293	many glycoproteins	276:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	0	42	theme	reversible	46:55	arg1	interactions					66:77	synergistic and reversible covalent interactions	30:77	synergistic and reversible covalent interactions for large-scale analysis of glycoproteins	30:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	0	43	theme	covalent	57:64	arg1	interactions					66:77	synergistic and reversible covalent interactions	30:77	synergistic and reversible covalent interactions for large-scale analysis of glycoproteins	30:119	An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.					
29703890	1	44	theme	Protein	122:128	arg1	glycosylation					130:142	Protein glycosylation	122:142	Protein glycosylation	122:142	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	3	45	theme	Chemical	329:336	arg1	methods					338:344	Chemical methods	329:344	Chemical methods based on reversible covalent interactions between boronic acid and glycans	329:419	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	6	46	theme	synergistic	914:924	arg1	interactions					947:958	synergistic benzoboroxole-glycan interactions	914:958	synergistic benzoboroxole-glycan interactions	914:958	The enrichment is further improved by conjugating benzoboroxole to a dendrimer, which enables synergistic benzoboroxole-glycan interactions.					
29703890	8	47	theme	current	1167:1173	arg1	method					1175:1180	the current method	1163:1180	the current method	1163:1180	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	8	48	theme	glycan	1269:1274	arg1	structures					1276:1285	glycan structures	1269:1285	glycan structures	1269:1285	Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.					
29703890	5	49	gly	glycopeptide	773:784	arg2	glycopeptide					773:784	glycopeptide coverage	773:793	glycopeptide coverage from human cell lysates	773:817	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29703890	4	50	theme	glycopeptide	641:652	arg1	enrichment					654:663	the glycopeptide enrichment	637:663	the glycopeptide enrichment	637:663	Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment.					
29703890	2	51	theme	low	259:261	arg1	abundance					263:271	the low abundance	255:271	the low abundance of many glycoproteins	255:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	7	52	theme	low-abundance	1072:1084	arg1	glycopeptides					1086:1098	low-abundance glycopeptides	1072:1098	low-abundance glycopeptides	1072:1098	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	2	53	gly	heterogeneity	226:238	arg1	glycans					243:249	glycans	243:249	glycans	243:249	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	2	53	gly	heterogeneity	226:238	arg1	glycoproteins					281:293	many glycoproteins	276:293	many glycoproteins	276:293	However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis.					
29703890	3	54	gly	glycopeptides	452:464	arg2	glycopeptides					452:464	glycopeptides	452:464	glycopeptides	452:464	Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species.					
29703890	1	55	theme	biological	161:170	arg1	systems					172:178	biological systems	161:178	biological systems	161:178	Protein glycosylation is ubiquitous in biological systems and essential for cell survival.					
29703890	7	56	theme	simple	977:982	arg1	method					984:989	This robust and simple method	961:989	This robust and simple method	961:989	This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides.					
29703890	5	57	theme	human	800:804	arg1	lysates					811:817	human cell lysates	800:817	human cell lysates	800:817	We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates.					
29675740	3	0	theme	-based	739:744	arg1	method					768:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	2	1	theme	analyses	651:658	arg1	throughput					633:642	throughput	633:642	throughput	633:642	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	2	1	theme	analyses	651:658	arg1	sensitivity					617:627	sensitivity	617:627	sensitivity	617:627	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	5	2	gly	glycopeptides	1129:1141	arg2	glycopeptides					1129:1141	sample glycopeptides	1122:1141	sample glycopeptides	1122:1141	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	1	3	theme	glycosylation	305:317	arg1	sites					319:323	glycosylation sites	305:323	glycosylation sites	305:323	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	1	4	theme	protein	214:220	arg1	function					222:229	the protein function	210:229	the protein function	210:229	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	4	5	theme	accurate	940:947	arg1	masses					949:954	accurate masses	940:954	accurate masses	940:954	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	3	6	theme	Glycan	812:817	arg1	IDentification					850:863	Glycan heterogeneity-based Relational IDentification	812:863	Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile	812:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	6	theme	Glycan	812:817	arg1	Glyco-RIDGE					909:919	Glyco-RIDGE	909:919	Glyco-RIDGE	909:919	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	10	7	theme	glycoproteome	1881:1893	arg1	analysis					1865:1872	the analysis	1861:1872	the analysis of the glycoproteome using our novel Glyco-RIDGE method	1861:1928	Thus, these findings provided important insights into the analysis of the glycoproteome using our novel Glyco-RIDGE method.					
29675740	1	8	gly	glycoprotein	387:398	arg1	glycoprotein					387:398	the glycoprotein	383:398	the glycoprotein	383:398	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	6	9	gly	glycopeptides	1217:1229	arg2	glycopeptides					1217:1229	glycopeptides	1217:1229	glycopeptides	1217:1229	Therefore, in this study, glycopeptides were fractionated into four fractions by hydrophilic interaction chromatography, and each fraction was analyzed using the Glyco-RIDGE method.					
29675740	5	10	theme	sample	1160:1165	arg1	complexity					1167:1176	sample complexity	1160:1176	sample complexity	1160:1176	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	10	11	theme	Glyco-RIDGE	1911:1921	arg1	method					1923:1928	our novel Glyco-RIDGE method	1901:1928	our novel Glyco-RIDGE method	1901:1928	Thus, these findings provided important insights into the analysis of the glycoproteome using our novel Glyco-RIDGE method.					
29675740	4	12	theme	glycopeptides	979:991	arg1	masses					949:954	accurate masses	940:954	accurate masses	940:954	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	4	12	theme	glycopeptides	979:991	arg1	times					970:974	retention times	960:974	retention times	960:974	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	7	13	theme	hydrophilic	1454:1464	arg1	fraction					1466:1473	the highest hydrophilic fraction	1442:1473	the highest hydrophilic fraction	1442:1473	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	3	14	theme	Relational	839:848	arg1	IDentification					850:863	Glycan heterogeneity-based Relational IDentification	812:863	Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile	812:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	14	theme	Relational	839:848	arg1	Glyco-RIDGE					909:919	Glyco-RIDGE	909:919	Glyco-RIDGE	909:919	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	5	15	theme	sample	1122:1127	arg1	glycopeptides					1129:1141	sample glycopeptides	1122:1141	sample glycopeptides	1122:1141	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	3	16	theme	Elution	892:898	arg1	profile					900:906	Elution profile	892:906	Elution profile	892:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	5	17	theme	glycopeptides	1129:1141	arg1	fractionation					1105:1117	fractionation	1105:1117	fractionation of sample glycopeptides for reduction of sample complexity	1105:1176	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	2	18	theme	spectra	553:559	arg1	analyses					533:540	complicated analyses	521:540	complicated analyses of complex spectra obtained by multistage fragmentation	521:596	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	6	19	theme	interaction	1284:1294	arg1	chromatography					1296:1309	hydrophilic interaction chromatography	1272:1309	hydrophilic interaction chromatography	1272:1309	Therefore, in this study, glycopeptides were fractionated into four fractions by hydrophilic interaction chromatography, and each fraction was analyzed using the Glyco-RIDGE method.					
29675740	3	20	theme	signals	881:887	arg1	IDentification					850:863	Glycan heterogeneity-based Relational IDentification	812:863	Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile	812:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	20	theme	signals	881:887	arg1	Glyco-RIDGE					909:919	Glyco-RIDGE	909:919	Glyco-RIDGE	909:919	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	0	21	theme	Leukemia	97:104	arg1	Cells					106:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	3	22	dep	glycome	803:809	arg1	IDentification					850:863	Glycan heterogeneity-based Relational IDentification	812:863	Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile	812:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	22	dep	glycome	803:809	arg1	Glyco-RIDGE					909:919	Glyco-RIDGE	909:919	Glyco-RIDGE	909:919	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	4	23	gly	glycopeptides	979:991	arg2	glycopeptides					979:991	glycopeptides	979:991	glycopeptides	979:991	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	0	24	from	Identification	0:13	arg1	Cells					106:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	0	25	theme	Site-Specific	120:132	arg1	Method					151:156	a Site-Specific Glycome Analysis Method	118:156	a Site-Specific Glycome Analysis Method	118:156	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	8	26	theme	pLN-carrier	1607:1617	arg1	proteins					1619:1626	31 pLN-carrier proteins	1604:1626	31 pLN-carrier proteins	1604:1626	Based on the monosaccharide composition, these glycans were thought to be poly-N-acetyllactosamine (polylactosamine [pLN]), and 31 pLN-carrier proteins were identified in HL-60 cells.					
29675740	2	27	theme	structural	437:446	arg1	technology					457:466	Glycopeptide-based structural analysis technology	418:466	Glycopeptide-based structural analysis technology using mass spectrometry	418:490	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	0	28	theme	Analysis	142:149	arg1	Method					151:156	a Site-Specific Glycome Analysis Method	118:156	a Site-Specific Glycome Analysis Method	118:156	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	1	29	gly	glycosylation	305:317	arg2	sites					319:323	glycosylation sites	305:323	glycosylation sites	305:323	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	0	30	dep	Using	112:116	arg1	Glyco-RIDGE					159:169	Glyco-RIDGE	159:169	Glyco-RIDGE	159:169	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	7	31	theme	long	1412:1415	arg1	glycans					1417:1423	long glycans	1412:1423	long glycans	1412:1423	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	2	32	theme	mass	474:477	arg1	spectrometry					479:490	mass spectrometry	474:490	mass spectrometry	474:490	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	0	33	theme	Poly-N-Acetyllactosamine-Carrying	18:50	arg1	Glycoproteins					52:64	Poly-N-Acetyllactosamine-Carrying Glycoproteins	18:64	Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells	18:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	1	34	theme	glycan	326:331	arg1	variation					333:341	glycan variation	326:341	glycan variation	326:341	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	2	35	theme	complicated	521:531	arg1	analyses					533:540	complicated analyses	521:540	complicated analyses of complex spectra obtained by multistage fragmentation	521:596	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	3	36	theme	chromatography/mass	702:720	arg1	MS					736:737	MS	736:737	MS	736:737	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	36	theme	chromatography/mass	702:720	arg1	spectrometry					722:733	a liquid chromatography/mass spectrometry	693:733	a liquid chromatography/mass spectrometry (MS)	693:738	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	4	37	used	used	935:938	arg2	method					928:933	This method	923:933	This method	923:933	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	5	38	theme	peptide	1096:1102	arg1	number					1075:1080	the number	1071:1080	the number of identified peptide	1071:1102	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	4	39	theme	complex	1041:1047	arg1	mixtures					1049:1056	complex mixtures	1041:1056	complex mixtures	1041:1056	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	0	40	theme	Human	77:81	arg1	Cells					106:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	1	41	theme	glycan	348:353	arg1	proportions					355:365	glycan proportions	348:365	glycan proportions	348:365	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	9	42	theme	ontology	1665:1672	arg1	analysis					1685:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	9	43	theme	many	1730:1733	arg1	molecules					1735:1743	many molecules	1730:1743	many molecules related to signal transduction, receptors, and cell adhesion	1730:1804	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	10	44	theme	important	1837:1845	arg1	insights					1847:1854	important insights	1837:1854	important insights into the analysis of the glycoproteome using our novel Glyco-RIDGE method	1837:1928	Thus, these findings provided important insights into the analysis of the glycoproteome using our novel Glyco-RIDGE method.					
29675740	9	45	theme	related	1745:1751	arg1	molecules					1735:1743	many molecules	1730:1743	many molecules related to signal transduction, receptors, and cell adhesion	1730:1804	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	9	46	theme	signal	1756:1761	arg1	transduction					1763:1774	signal transduction	1756:1774	signal transduction	1756:1774	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	3	47	theme	glycopeptide	746:757	arg1	method					768:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	4	48	theme	retention	960:968	arg1	times					970:974	retention times	960:974	retention times	960:974	This method used accurate masses and retention times of glycopeptides, without requiring MS2, and could be applied to complex mixtures.					
29675740	3	49	theme	site-specific	789:801	arg1	glycome					803:809	the site-specific glycome	785:809	the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE)	785:920	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	6	50	theme	Glyco-RIDGE	1353:1363	arg1	method					1365:1370	the Glyco-RIDGE method	1349:1370	the Glyco-RIDGE method	1349:1370	Therefore, in this study, glycopeptides were fractionated into four fractions by hydrophilic interaction chromatography, and each fraction was analyzed using the Glyco-RIDGE method.					
29675740	1	51	gly	heterogeneity	253:265	arg1	glycans					270:276	glycans	270:276	glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein	270:398	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	9	52	theme	cell	1792:1795	arg1	adhesion					1797:1804	cell adhesion	1792:1804	cell adhesion	1792:1804	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	3	53	theme	heterogeneity-based	819:837	arg1	IDentification					850:863	Glycan heterogeneity-based Relational IDentification	812:863	Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile	812:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	53	theme	heterogeneity-based	819:837	arg1	Glyco-RIDGE					909:919	Glyco-RIDGE	909:919	Glyco-RIDGE	909:919	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	0	54	from	Cells	106:110	arg1	Identification					0:13	Identification	0:13	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells	0:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	0	54	from	Cells	106:110	arg1	Glycoproteins					52:64	Poly-N-Acetyllactosamine-Carrying Glycoproteins	18:64	Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells	18:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	3	55	theme	Glycopeptide	868:879	arg1	signals					881:887	Glycopeptide signals	868:887	Glycopeptide signals	868:887	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	5	56	theme	complexity	1167:1176	arg1	reduction					1147:1155	reduction	1147:1155	reduction of sample complexity	1147:1176	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	10	57	theme	novel	1905:1909	arg1	method					1923:1928	our novel Glyco-RIDGE method	1901:1928	our novel Glyco-RIDGE method	1901:1928	Thus, these findings provided important insights into the analysis of the glycoproteome using our novel Glyco-RIDGE method.					
29675740	8	58	theme	polylactosamine	1576:1590	arg1	poly-N-acetyllactosamine					1550:1573	poly-N-acetyllactosamine	1550:1573	poly-N-acetyllactosamine (polylactosamine [pLN])	1550:1597	Based on the monosaccharide composition, these glycans were thought to be poly-N-acetyllactosamine (polylactosamine [pLN]), and 31 pLN-carrier proteins were identified in HL-60 cells.					
29675740	8	58	theme	polylactosamine	1576:1590	arg1	[pLN					1592:1595	polylactosamine [pLN	1576:1595	polylactosamine [pLN	1576:1595	Based on the monosaccharide composition, these glycans were thought to be poly-N-acetyllactosamine (polylactosamine [pLN]), and 31 pLN-carrier proteins were identified in HL-60 cells.					
29675740	11	59	theme	Graphical	1931:1939	arg1	Abstract					1941:1948	Graphical Abstract	1931:1948	Graphical Abstract	1931:1948	Graphical Abstract ᅟ.					
29675740	7	60	contain	having	1405:1410	arg2	glycans					1417:1423	long glycans	1412:1423	long glycans	1412:1423	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	7	60	contain	having	1405:1410	arg1	result					1378:1383	a result	1376:1383	a result	1376:1383	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	7	60	contain	having	1405:1410	arg1	glycopeptides					1391:1403	many glycopeptides	1386:1403	many glycopeptides having long glycans	1386:1423	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	7	61	theme	highest	1446:1452	arg1	fraction					1466:1473	the highest hydrophilic fraction	1442:1473	the highest hydrophilic fraction	1442:1473	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	2	62	theme	complex	545:551	arg1	spectra					553:559	complex spectra	545:559	complex spectra obtained by multistage fragmentation	545:596	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	6	63	theme	hydrophilic	1272:1282	arg1	chromatography					1296:1309	hydrophilic interaction chromatography	1272:1309	hydrophilic interaction chromatography	1272:1309	Therefore, in this study, glycopeptides were fractionated into four fractions by hydrophilic interaction chromatography, and each fraction was analyzed using the Glyco-RIDGE method.					
29675740	7	64	gly	glycopeptides	1391:1403	arg2	result					1378:1383	a result	1376:1383	a result	1376:1383	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	7	64	gly	glycopeptides	1391:1403	arg2	glycopeptides					1391:1403	many glycopeptides	1386:1403	many glycopeptides having long glycans	1386:1423	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	3	65	from	IDentification	850:863	arg1	profile					900:906	Elution profile	892:906	Elution profile	892:906	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	0	66	theme	Glycome	134:140	arg1	Method					151:156	a Site-Specific Glycome Analysis Method	118:156	a Site-Specific Glycome Analysis Method	118:156	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	2	67	theme	multistage	573:582	arg1	fragmentation					584:596	multistage fragmentation	573:596	multistage fragmentation	573:596	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	1	68	theme	glycoprotein	387:398	arg1	site					375:378	each site	370:378	each site of the glycoprotein	370:398	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	1	68	theme	glycoprotein	387:398	arg1	glycoprotein					387:398	the glycoprotein	383:398	the glycoprotein	383:398	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	2	69	theme	Glycopeptide-based	418:435	arg1	technology					457:466	Glycopeptide-based structural analysis technology	418:466	Glycopeptide-based structural analysis technology using mass spectrometry	418:490	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	9	70	theme	Gene	1660:1663	arg1	analysis					1685:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	8	71	theme	HL-60	1647:1651	arg1	cells					1653:1657	HL-60 cells	1647:1657	HL-60 cells	1647:1657	Based on the monosaccharide composition, these glycans were thought to be poly-N-acetyllactosamine (polylactosamine [pLN]), and 31 pLN-carrier proteins were identified in HL-60 cells.					
29675740	0	72	theme	Glycoproteins	52:64	arg1	Identification					0:13	Identification	0:13	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells	0:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	9	73	theme	enrichment	1674:1683	arg1	analysis					1685:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis	1660:1692	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	7	74	theme	many	1386:1389	arg1	result					1378:1383	a result	1376:1383	a result	1376:1383	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	7	74	theme	many	1386:1389	arg1	glycopeptides					1391:1403	many glycopeptides	1386:1403	many glycopeptides having long glycans	1386:1423	As a result, many glycopeptides having long glycans were enriched in the highest hydrophilic fraction.					
29675740	3	75	theme	liquid	695:700	arg1	MS					736:737	MS	736:737	MS	736:737	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	3	75	theme	liquid	695:700	arg1	spectrometry					722:733	a liquid chromatography/mass spectrometry	693:733	a liquid chromatography/mass spectrometry (MS)	693:738	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	0	76	theme	HL-60	71:75	arg1	Cells					106:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	5	77	theme	identified	1085:1094	arg1	peptide					1096:1102	identified peptide	1085:1102	identified peptide	1085:1102	To increase the number of identified peptide, fractionation of sample glycopeptides for reduction of sample complexity is required.					
29675740	2	78	theme	analysis	448:455	arg1	technology					457:466	Glycopeptide-based structural analysis technology	418:466	Glycopeptide-based structural analysis technology using mass spectrometry	418:490	Glycopeptide-based structural analysis technology using mass spectrometry has been developed; however, complicated analyses of complex spectra obtained by multistage fragmentation are necessary, and sensitivity and throughput of the analyses are low.					
29675740	0	79	theme	Promyelocytic	83:95	arg1	Cells					106:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	HL-60 Human Promyelocytic Leukemia Cells	71:110	Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE.					
29675740	9	80	theme	pLN	1708:1710	arg1	carriers					1712:1719	pLN carriers	1708:1719	pLN carriers	1708:1719	Gene ontology enrichment analysis revealed that pLN carriers included many molecules related to signal transduction, receptors, and cell adhesion.					
29675740	3	81	gly	glycopeptide	746:757	arg2	glycopeptide					746:757	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	8	82	theme	monosaccharide	1489:1502	arg1	composition					1504:1514	the monosaccharide composition	1485:1514	the monosaccharide composition	1485:1514	Based on the monosaccharide composition, these glycans were thought to be poly-N-acetyllactosamine (polylactosamine [pLN]), and 31 pLN-carrier proteins were identified in HL-60 cells.					
29675740	1	83	theme	glycans	270:276	arg1	diversity					239:247	diversity	239:247	diversity	239:247	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	1	83	theme	glycans	270:276	arg1	heterogeneity					253:265	heterogeneity	253:265	heterogeneity	253:265	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	1	84	dep	analyzed	408:415	arg1	elucidate					175:183	elucidate	175:183	To elucidate the relationship between the protein function	172:229	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29675740	3	85	theme	analysis	759:766	arg1	method					768:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method	693:773	Therefore, we developed a liquid chromatography/mass spectrometry (MS)-based glycopeptide analysis method to reveal the site-specific glycome (Glycan heterogeneity-based Relational IDentification of Glycopeptide signals on Elution profile, Glyco-RIDGE).					
29675740	1	86	dep	diversity	239:247	arg1	the					235:237	the	235:237	the	235:237	To elucidate the relationship between the protein function and the diversity and heterogeneity of glycans conjugated to the protein, glycosylation sites, glycan variation, and glycan proportions at each site of the glycoprotein must be analyzed.					
29769321	6	0	theme	hemagglutination	1037:1052	arg1	inhibition					1054:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	7	1	theme	branched	1432:1439	arg1	oligosaccharides					1477:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	4	2	theme	HEK293	819:824	arg1	cells					826:830	HEK293 cells	819:830	HEK293 cells	819:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	10	3	from	presence	1681:1688	arg1	CRD					1713:1715	the CRD	1709:1715	the CRD	1709:1715	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	6	4	theme	pandemic	1227:1234	arg1	A/Aichi/68					1236:1245	pandemic A/Aichi/68	1227:1245	pandemic A/Aichi/68 (H3N2)	1227:1252	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	4	theme	pandemic	1227:1234	arg1	H3N2					1248:1251	H3N2	1248:1251	H3N2	1248:1251	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	4	5	theme	porcine	794:800	arg1	SP-D					802:805	porcine SP-D	794:805	porcine SP-D (RpNCRD) in HEK293 cells	794:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	4	5	theme	porcine	794:800	arg1	RpNCRD					808:813	RpNCRD	808:813	RpNCRD	808:813	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	7	6	theme	%	1374:1374	arg1	site-occupancy					1353:1366	an N-glycan site-occupancy	1341:1366	an N-glycan site-occupancy of >98%	1341:1374	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	3	7	theme	N-linked	473:480	arg1	glycosylation					486:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	1	8	theme	A	185:185	arg1	virus					187:191	influenza A virus	175:191	influenza A virus (IAV) infection	175:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	1	8	theme	A	185:185	arg1	IAV					194:196	IAV	194:196	IAV	194:196	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	6	9	theme	infection	1109:1117	arg1	studies					1119:1125	IAV infection studies	1105:1125	IAV infection studies	1105:1125	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	10	theme	potent	1190:1195	arg1	activity					1210:1217	potent neutralizing activity	1190:1217	potent neutralizing activity against pandemic A/Aichi/68 (H3N2)	1190:1252	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	7	11	theme	N-glycan	1344:1351	arg1	site-occupancy					1353:1366	an N-glycan site-occupancy	1341:1366	an N-glycan site-occupancy of >98%	1341:1374	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	6	12	theme	structural	1288:1297	arg1	features					1299:1306	both porcine-specific structural features	1266:1306	both porcine-specific structural features of its CRD	1266:1317	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	10	13	theme	RpNCRD	1761:1766	arg1	specificity					1746:1756	the glycan-binding specificity	1727:1756	the glycan-binding specificity of RpNCRD	1727:1766	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	11	14	theme	SP-D-based	1908:1917	arg1	drugs					1929:1933	recombinant SP-D-based antiviral drugs	1896:1933	recombinant SP-D-based antiviral drugs	1896:1933	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	11	15	theme	defense	1838:1844	arg1	understanding					1797:1809	our understanding	1793:1809	our understanding of porcine-specific innate defense against pandemic IAV	1793:1865	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	4	16	from	fragment	782:789	arg1	cells					826:830	HEK293 cells	819:830	HEK293 cells	819:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	1	17	theme	crucial	250:256	arg1	role					258:261	a crucial role	248:261	a crucial role	248:261	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	7	18	theme	MS	1320:1321	arg1	analysis					1323:1330	MS analysis	1320:1330	MS analysis	1320:1330	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	11	19	theme	porcine-specific	1814:1829	arg1	defense					1838:1844	porcine-specific innate defense	1814:1844	porcine-specific innate defense against pandemic IAV	1814:1865	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	3	20	theme	acid-binding	538:549	arg1	site					551:554	the sialic acid-binding site	527:554	the sialic acid-binding site of IAV	527:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	20	theme	acid-binding	538:549	arg1	IAV					559:561	IAV	559:561	IAV	559:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	0	21	theme	Lectin-mediated	0:14	arg1	binding					16:22	Lectin-mediated binding	0:22	Lectin-mediated binding	0:22	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	6	22	theme	human	1158:1162	arg1	RhNCRD					1173:1178	the human analogue RhNCRD	1154:1178	the human analogue RhNCRD	1154:1178	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	5	23	theme	second	990:995	arg1	site					1015:1018	a potential second nonlectin-binding site	978:1018	a potential second nonlectin-binding site for glycans	978:1030	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	0	24	theme	D	71:71	arg1	sialoglycans					28:39	sialoglycans	28:39	sialoglycans	28:39	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	0	24	theme	D	71:71	arg1	binding					16:22	Lectin-mediated binding	0:22	Lectin-mediated binding	0:22	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	1	25	theme	early	154:158	arg1	containment					160:170	the early containment	150:170	the early containment of influenza A virus (IAV) infection	150:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	1	26	from	containment	160:170	arg1	critical					138:145	critical	138:145	critical	138:145	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	4	27	theme	neutralization	709:722	arg1	mechanisms					691:700	both mechanisms	686:700	both mechanisms of IAV neutralization	686:722	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	0	28	theme	A	110:110	arg1	virus					112:116	influenza A virus	100:116	influenza A virus	100:116	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	5	29	theme	backbone	905:912	arg1	structure					914:922	the CRD backbone structure	897:922	the CRD backbone structure	897:922	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	4	30	theme	greater	727:733	arg1	detail					735:740	greater detail	727:740	greater detail	727:740	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	11	31	theme	antiviral	1919:1927	arg1	drugs					1929:1933	recombinant SP-D-based antiviral drugs	1896:1933	recombinant SP-D-based antiviral drugs	1896:1933	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	1	32	from	critical	138:145	arg1	containment					160:170	the early containment	150:170	the early containment of influenza A virus (IAV) infection	150:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	11	33	theme	recombinant	1896:1906	arg1	drugs					1929:1933	recombinant SP-D-based antiviral drugs	1896:1933	recombinant SP-D-based antiviral drugs	1896:1933	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	4	34	gly	N-glycosylated	758:771	arg1	fragment					782:789	an N-glycosylated neck-CRD fragment	755:789	an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells	755:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	4	35	from	cells	826:830	arg1	fragment					782:789	an N-glycosylated neck-CRD fragment	755:789	an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells	755:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	9	36	theme	polylactosamine-containing	1641:1666	arg1	glycans					1668:1674	polylactosamine-containing glycans	1641:1674	polylactosamine-containing glycans	1641:1674	RpNCRD also bound LewisY structures, whereas RhNCRD bound polylactosamine-containing glycans.					
29769321	8	37	theme	array	1510:1514	arg1	data					1516:1519	Glycan-binding array data	1495:1519	Glycan-binding array data	1495:1519	Glycan-binding array data characterized both RpNCRD and RhNCRD as mannose-type lectins.					
29769321	2	38	theme	IAVs	383:386	arg1	generation					360:369	the generation	356:369	the generation of pandemic IAVs	356:386	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	1	39	theme	Innate	119:124	arg1	immunity					126:133	Innate immunity	119:133	Innate immunity	119:133	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	3	40	with	interactions	509:520	arg1	site					551:554	the sialic acid-binding site	527:554	the sialic acid-binding site of IAV	527:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	40	with	interactions	509:520	arg1	IAV					559:561	IAV	559:561	IAV	559:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	4	41	theme	N-glycosylated	758:771	arg1	fragment					782:789	an N-glycosylated neck-CRD fragment	755:789	an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells	755:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	7	42	theme	-sialylated	1465:1475	arg1	oligosaccharides					1477:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	2	43	theme	carbohydrate	410:421	arg1	CRD					443:445	CRD	443:445	CRD	443:445	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	2	43	theme	carbohydrate	410:421	arg1	domain					435:440	its unique carbohydrate recognition domain	399:440	its unique carbohydrate recognition domain (CRD)	399:446	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	10	44	theme	N-glycan	1697:1704	arg1	presence					1681:1688	The presence	1677:1688	The presence of the N-glycan in the CRD	1677:1715	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	0	45	theme	surfactant	52:61	arg1	D					71:71	porcine surfactant protein D	44:71	porcine surfactant protein D	44:71	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	10	46	attach	presence	1681:1688	arg1	CRD					1713:1715	the CRD	1709:1715	the CRD	1709:1715	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	10	46	attach	presence	1681:1688	arg2	N-glycan					1697:1704	the N-glycan	1693:1704	the N-glycan	1693:1704	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	3	47	theme	enhanced	625:632	arg1	interactions					634:645	enhanced interactions	625:645	enhanced interactions with IAV glycans	625:662	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	11	48	theme	pandemic	1854:1861	arg1	IAV					1863:1865	pandemic IAV	1854:1865	pandemic IAV	1854:1865	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	6	49	theme	IAV	1066:1068	arg1	aggregation					1070:1080	IAV aggregation	1066:1080	IAV aggregation	1066:1080	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	8	50	theme	mannose-type	1561:1572	arg1	RhNCRD					1551:1556	RhNCRD	1551:1556	RhNCRD	1551:1556	Glycan-binding array data characterized both RpNCRD and RhNCRD as mannose-type lectins.					
29769321	8	50	theme	mannose-type	1561:1572	arg1	lectins					1574:1580	mannose-type lectins	1561:1580	mannose-type lectins	1561:1580	Glycan-binding array data characterized both RpNCRD and RhNCRD as mannose-type lectins.					
29769321	8	50	theme	mannose-type	1561:1572	arg1	RpNCRD					1540:1545	RpNCRD	1540:1545	RpNCRD	1540:1545	Glycan-binding array data characterized both RpNCRD and RhNCRD as mannose-type lectins.					
29769321	2	51	theme	important	320:328	arg1	pigs					304:307	pigs	304:307	pigs	304:307	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	2	51	theme	important	320:328	arg1	hosts					343:347	important intermediate hosts	320:347	important intermediate hosts	320:347	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	1	52	theme	protein	225:231	arg1	SP-D					236:239	SP-D	236:239	SP-D	236:239	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	1	52	theme	protein	225:231	arg1	D					233:233	surfactant protein D	214:233	surfactant protein D (SP-D)	214:240	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	0	53	theme	protein	63:69	arg1	D					71:71	porcine surfactant protein D	44:71	porcine surfactant protein D	44:71	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	5	54	theme	site	946:949	arg1	conformation					951:962	the lectin site conformation	935:962	the lectin site conformation	935:962	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	6	55	theme	IAV	1033:1035	arg1	inhibition					1054:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	3	56	theme	CRD	482:484	arg1	glycosylation					486:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	7	57	theme	complex-type	1402:1413	arg1	oligosaccharides					1477:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	10	58	theme	glycan-binding	1731:1744	arg1	specificity					1746:1756	the glycan-binding specificity	1727:1756	the glycan-binding specificity of RpNCRD	1727:1766	The presence of the N-glycan in the CRD increases the glycan-binding specificity of RpNCRD.					
29769321	1	59	theme	influenza	175:183	arg1	virus					187:191	influenza A virus	175:191	influenza A virus (IAV) infection	175:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	1	59	theme	influenza	175:183	arg1	IAV					194:196	IAV	194:196	IAV	194:196	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	7	60	theme	RpNCRD	1390:1395	arg1	Asn-303					1379:1385	Asn-303	1379:1385	Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1379:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	6	61	theme	studies	1119:1125	arg1	inhibition					1054:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition	1033:1063	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	61	theme	studies	1119:1125	arg1	aggregation					1070:1080	IAV aggregation	1066:1080	IAV aggregation	1066:1080	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	61	theme	studies	1119:1125	arg1	neutralization					1087:1100	neutralization	1087:1100	neutralization of IAV infection studies	1087:1125	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	62	theme	neutralizing	1197:1208	arg1	activity					1210:1217	potent neutralizing activity	1190:1217	potent neutralizing activity against pandemic A/Aichi/68 (H3N2)	1190:1252	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	1	63	theme	virus	187:191	arg1	infection					199:207	influenza A virus (IAV) infection	175:207	influenza A virus (IAV) infection	175:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	4	64	theme	SP-D	802:805	arg1	fragment					782:789	an N-glycosylated neck-CRD fragment	755:789	an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells	755:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	6	65	theme	IAV	1105:1107	arg1	studies					1119:1125	IAV infection studies	1105:1125	IAV infection studies	1105:1125	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	6	66	theme	porcine-specific	1271:1286	arg1	features					1299:1306	both porcine-specific structural features	1266:1306	both porcine-specific structural features of its CRD	1266:1317	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	1	67	theme	infection	199:207	arg1	containment					160:170	the early containment	150:170	the early containment of influenza A virus (IAV) infection	150:207	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	3	68	theme	sialic	531:536	arg1	site					551:554	the sialic acid-binding site	527:554	the sialic acid-binding site of IAV	527:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	68	theme	sialic	531:536	arg1	IAV					559:561	IAV	559:561	IAV	559:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	6	69	theme	analogue	1164:1171	arg1	RhNCRD					1173:1178	the human analogue RhNCRD	1154:1178	the human analogue RhNCRD	1154:1178	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	11	70	theme	innate	1831:1836	arg1	defense					1838:1844	porcine-specific innate defense	1814:1844	porcine-specific innate defense against pandemic IAV	1814:1865	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	5	71	theme	potential	980:988	arg1	site					1015:1018	a potential second nonlectin-binding site	978:1018	a potential second nonlectin-binding site for glycans	978:1030	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	5	72	theme	nonlectin-binding	997:1013	arg1	site					1015:1018	a potential second nonlectin-binding site	978:1018	a potential second nonlectin-binding site for glycans	978:1030	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	3	73	theme	IAV	559:561	arg1	site					551:554	the sialic acid-binding site	527:554	the sialic acid-binding site of IAV	527:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	73	theme	IAV	559:561	arg1	IAV					559:561	IAV	559:561	IAV	559:561	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	6	74	theme	CRD	1315:1317	arg1	features					1299:1306	both porcine-specific structural features	1266:1306	both porcine-specific structural features of its CRD	1266:1317	IAV hemagglutination inhibition, IAV aggregation, and neutralization of IAV infection studies showed that RpNCRD, unlike the human analogue RhNCRD, exhibits potent neutralizing activity against pandemic A/Aichi/68 (H3N2), enabled by both porcine-specific structural features of its CRD.					
29769321	0	75	theme	influenza	100:108	arg1	virus					112:116	influenza A virus	100:116	influenza A virus	100:116	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	5	76	theme	X-ray	833:837	arg1	crystallography					839:853	X-ray crystallography	833:853	X-ray crystallography	833:853	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	5	77	theme	CRD	901:903	arg1	structure					914:922	the CRD backbone structure	897:922	the CRD backbone structure	897:922	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	7	78	gly	-sialylated	1465:1475	arg1	oligosaccharides					1477:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	3	79	link	N-linked	473:480	arg1	glycosylation					486:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation	470:498	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	4	80	theme	IAV	705:707	arg1	neutralization					709:722	IAV neutralization	705:722	IAV neutralization	705:722	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	4	81	from	SP-D	802:805	arg1	cells					826:830	HEK293 cells	819:830	HEK293 cells	819:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	8	82	theme	Glycan-binding	1495:1508	arg1	data					1516:1519	Glycan-binding array data	1495:1519	Glycan-binding array data	1495:1519	Glycan-binding array data characterized both RpNCRD and RhNCRD as mannose-type lectins.					
29769321	11	83	theme	drugs	1929:1933	arg1	design					1886:1891	the design	1882:1891	the design of recombinant SP-D-based antiviral drugs	1882:1933	These insights increase our understanding of porcine-specific innate defense against pandemic IAV and may inform the design of recombinant SP-D-based antiviral drugs.					
29769321	2	84	theme	intermediate	330:341	arg1	pigs					304:307	pigs	304:307	pigs	304:307	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	2	84	theme	intermediate	330:341	arg1	hosts					343:347	important intermediate hosts	320:347	important intermediate hosts	320:347	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	9	85	theme	LewisY	1601:1606	arg1	structures					1608:1617	LewisY structures	1601:1617	LewisY structures	1601:1617	RpNCRD also bound LewisY structures, whereas RhNCRD bound polylactosamine-containing glycans.					
29769321	2	86	theme	pandemic	374:381	arg1	IAVs					383:386	pandemic IAVs	374:386	pandemic IAVs	374:386	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	0	87	theme	porcine	44:50	arg1	D					71:71	porcine surfactant protein D	44:71	porcine surfactant protein D	44:71	Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.					
29769321	2	88	theme	recognition	423:433	arg1	CRD					443:445	CRD	443:445	CRD	443:445	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	2	88	theme	recognition	423:433	arg1	domain					435:440	its unique carbohydrate recognition domain	399:440	its unique carbohydrate recognition domain (CRD)	399:446	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	3	89	theme	tripeptide	570:579	arg1	loop					581:584	a tripeptide loop	568:584	a tripeptide loop at the lectin-binding site	568:611	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	7	90	with	Asn-303	1379:1385	arg1	oligosaccharides					1477:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides	1416:1492	MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides.					
29769321	1	91	theme	pulmonary	270:278	arg1	defense					280:286	the pulmonary defense	266:286	the pulmonary defense against IAV	266:298	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	2	92	theme	unique	403:408	arg1	CRD					443:445	CRD	443:445	CRD	443:445	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	2	92	theme	unique	403:408	arg1	domain					435:440	its unique carbohydrate recognition domain	399:440	its unique carbohydrate recognition domain (CRD)	399:446	In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV.					
29769321	4	93	theme	neck-CRD	773:780	arg1	fragment					782:789	an N-glycosylated neck-CRD fragment	755:789	an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells	755:830	Here, to investigate both mechanisms of IAV neutralization in greater detail, we produced an N-glycosylated neck-CRD fragment of porcine SP-D (RpNCRD) in HEK293 cells.					
29769321	3	94	theme	lectin-binding	593:606	arg1	site					608:611	the lectin-binding site	589:611	the lectin-binding site	589:611	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	1	95	theme	surfactant	214:223	arg1	SP-D					236:239	SP-D	236:239	SP-D	236:239	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	1	95	theme	surfactant	214:223	arg1	D					233:233	surfactant protein D	214:233	surfactant protein D (SP-D)	214:240	Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV.					
29769321	5	96	theme	lectin	939:944	arg1	conformation					951:962	the lectin site conformation	935:962	the lectin site conformation	935:962	X-ray crystallography disclosed that the N-glycan did not alter the CRD backbone structure, including the lectin site conformation, but revealed a potential second nonlectin-binding site for glycans.					
29769321	3	97	from	site	608:611	arg1	loop					581:584	a tripeptide loop	568:584	a tripeptide loop at the lectin-binding site	568:611	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	98	theme	IAV	652:654	arg1	glycans					656:662	IAV glycans	652:662	IAV glycans	652:662	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29769321	3	99	with	interactions	634:645	arg1	glycans					656:662	IAV glycans	652:662	IAV glycans	652:662	An N-linked CRD glycosylation provides interactions with the sialic acid-binding site of IAV, and a tripeptide loop at the lectin-binding site facilitates enhanced interactions with IAV glycans.					
29603480	0	0	theme	Human	115:119	arg1	Sperm					121:125	Human Sperm	115:125	Human Sperm	115:125	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	3	1	theme	C-6	675:677	arg1	antenna					679:685	the C-6 antenna	671:685	the C-6 antenna	671:685	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	1	2	theme	zona	182:185	arg1	ZP					198:199	ZP	198:199	ZP	198:199	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	1	2	theme	zona	182:185	arg1	pellucida					187:195	the zona pellucida	178:195	the zona pellucida (ZP) of human oocytes	178:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	6	3	theme	Further	949:955	arg1	studies					957:963	Further studies	949:963	Further studies with SLex -Lex and SLex	949:987	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	5	4	theme	-Lex	844:847	arg1	critical					859:866	critical	859:866	critical	859:866	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	5	4	theme	-Lex	844:847	arg1	moiety					849:854	the SLex -Lex moiety	835:854	the SLex -Lex moiety	835:854	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	1	5	theme	pellucida	187:195	arg1	glycoproteins					161:173	glycoproteins	161:173	glycoproteins of the zona pellucida (ZP) of human oocytes	161:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	1	6	theme	spermatozoa	258:268	arg1	binding					247:253	the binding	243:253	the binding of spermatozoa	243:268	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	7	7	theme	inhibitory	1131:1140	arg1	activity					1142:1149	greater inhibitory activity	1123:1149	greater inhibitory activity	1123:1149	In addition, trivalent SLex -Lex and SLex were prepared which showed greater inhibitory activity compared to their monovalent counterparts.					
29603480	8	8	theme	productive	1418:1427	arg1	interactions					1429:1440	productive interactions	1418:1440	productive interactions	1418:1440	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	9	9	theme	various	1563:1569	arg1	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	9	theme	various	1563:1569	arg1	sites					1585:1589	the various glycosylation sites	1559:1589	the various glycosylation sites of ZP that accounts for high avidity binding	1559:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	2	10	theme	unusual	292:298	arg1	bi-					300:302	these unusual bi-	286:302	these unusual bi-	286:302	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	9	11	theme	multi-antennary	1490:1504	arg1	glycan					1506:1511	a multi-antennary glycan	1488:1511	a multi-antennary glycan	1488:1511	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	5	12	theme	other	905:909	arg1	moieties					916:923	the other SLex moieties	901:923	the other SLex moieties	901:923	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	5	13	theme	SLex	911:914	arg1	moieties					916:923	the other SLex moieties	901:923	the other SLex moieties	901:923	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	9	14	theme	high	1615:1618	arg1	binding					1628:1634	high avidity binding	1615:1634	high avidity binding	1615:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	3	15	theme	chemoenzymatic	490:503	arg1	synthesis					505:513	the chemoenzymatic synthesis	486:513	the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	486:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	5	16	theme	inhibitory	872:881	arg1	activity					883:890	inhibitory activity	872:890	inhibitory activity	872:890	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	3	17	theme	complex	525:531	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	9	18	gly	glycosylation	1571:1583	arg2	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	18	gly	glycosylation	1571:1583	arg1	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	18	gly	glycosylation	1571:1583	arg2	sites					1585:1589	the various glycosylation sites	1559:1589	the various glycosylation sites of ZP that accounts for high avidity binding	1559:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	7	19	theme	greater	1123:1129	arg1	activity					1142:1149	greater inhibitory activity	1123:1149	greater inhibitory activity	1123:1149	In addition, trivalent SLex -Lex and SLex were prepared which showed greater inhibitory activity compared to their monovalent counterparts.					
29603480	3	20	from	analogues	711:719	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	20	from	analogues	711:719	arg1	synthesis					505:513	the chemoenzymatic synthesis	486:513	the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	486:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	20	from	analogues	711:719	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	9	21	theme	multiple	1537:1544	arg1	-Lex					1551:1554	multiple SLex -Lex	1537:1554	multiple SLex -Lex	1537:1554	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	0	22	theme	Chemoenzymatic	0:13	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans	0:65	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	3	23	from	antenna	679:685	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	23	from	antenna	679:685	arg1	synthesis					505:513	the chemoenzymatic synthesis	486:513	the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	486:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	23	from	antenna	679:685	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	9	24	theme	ZP	1594:1595	arg1	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	24	theme	ZP	1594:1595	arg1	sites					1585:1589	the various glycosylation sites	1559:1589	the various glycosylation sites of ZP that accounts for high avidity binding	1559:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	25	theme	SLex	1480:1483	arg1	display					1469:1475	the multivalent display	1453:1475	the multivalent display of SLex on a multi-antennary glycan	1453:1511	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	25	theme	SLex	1480:1483	arg1	It					1443:1444	It	1443:1444	It	1443:1444	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	6	26	theme	extended	1005:1012	arg1	inhibitor					1043:1051	the more potent inhibitor	1027:1051	the more potent inhibitor	1027:1051	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	6	26	theme	extended	1005:1012	arg1	structure					1014:1022	the extended structure	1001:1022	the extended structure	1001:1022	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	2	27	theme	minimal	434:440	arg1	epitope					442:448	the minimal epitope	430:448	the minimal epitope for sperm binding	430:466	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	2	27	theme	minimal	434:440	arg1	tetrasaccharide					356:370	the tetrasaccharide sialyl-Lewisx	352:384	the tetrasaccharide sialyl-Lewisx (SLex )	352:392	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	8	28	theme	SLex	1399:1402	arg1	-Lex					1404:1407	SLex -Lex	1399:1407	SLex -Lex	1399:1407	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	2	29	theme	tri-	305:308	arg1	termini					275:281	The termini	271:281	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans	271:339	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	3	30	attach	derived	556:562	arg1	ZP					569:570	ZP	569:570	ZP carrying SLex moieties at the C-2 and C-2' arm	569:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	30	attach	derived	556:562	arg2	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	0	31	theme	Asymmetrical	28:39	arg1	N-Glycans					57:65	Asymmetrical Multi-Antennary N-Glycans	28:65	Asymmetrical Multi-Antennary N-Glycans	28:65	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	9	32	theme	SLex	1546:1549	arg1	-Lex					1551:1554	multiple SLex -Lex	1537:1554	multiple SLex -Lex	1537:1554	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	8	33	theme	complex	1314:1320	arg1	N-glycan					1322:1329	a complex N-glycan	1312:1329	a complex N-glycan	1312:1329	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	9	34	from	presentation	1521:1532	arg1	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	34	from	presentation	1521:1532	arg1	sites					1585:1589	the various glycosylation sites	1559:1589	the various glycosylation sites of ZP that accounts for high avidity binding	1559:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	1	35	theme	human	205:209	arg1	oocytes					211:217	human oocytes	205:217	human oocytes	205:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	3	36	theme	SLex	581:584	arg1	moieties					586:593	SLex moieties	581:593	SLex moieties	581:593	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	37	theme	related	703:709	arg1	analogues					711:719	two closely related analogues	691:719	two closely related analogues	691:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	38	theme	sialyl-Lewisx	625:637	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	38	theme	sialyl-Lewisx	625:637	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	2	39	theme	bi-	300:302	arg1	termini					275:281	The termini	271:281	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans	271:339	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	1	40	theme	oocytes	211:217	arg1	ZP					198:199	ZP	198:199	ZP	198:199	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	1	40	theme	oocytes	211:217	arg1	pellucida					187:195	the zona pellucida	178:195	the zona pellucida (ZP) of human oocytes	178:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	0	41	theme	N-Glycans	57:65	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans	0:65	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	3	42	contain	carrying	572:579	arg1	ZP					569:570	ZP	569:570	ZP carrying SLex moieties at the C-2 and C-2' arm	569:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	42	contain	carrying	572:579	arg2	moieties					586:593	SLex moieties	581:593	SLex moieties	581:593	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	1	43	gly	glycoproteins	161:173	arg1	glycoproteins					161:173	glycoproteins	161:173	glycoproteins of the zona pellucida (ZP) of human oocytes	161:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	3	44	theme	-Lewisx	639:645	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	44	theme	-Lewisx	639:645	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	8	45	theme	spermatozoa	1257:1267	arg1	binding					1246:1252	the binding	1242:1252	the binding of spermatozoa	1242:1267	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	7	46	theme	trivalent	1067:1075	arg1	-Lex					1082:1085	trivalent SLex -Lex	1067:1085	trivalent SLex -Lex	1067:1085	In addition, trivalent SLex -Lex and SLex were prepared which showed greater inhibitory activity compared to their monovalent counterparts.					
29603480	0	47	theme	Multi-Antennary	41:55	arg1	N-Glycans					57:65	Asymmetrical Multi-Antennary N-Glycans	28:65	Asymmetrical Multi-Antennary N-Glycans	28:65	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	9	48	theme	avidity	1620:1626	arg1	binding					1628:1634	high avidity binding	1615:1634	high avidity binding	1615:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	6	49	with	studies	957:963	arg1	SLex					984:987	SLex	984:987	SLex	984:987	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	6	49	with	studies	957:963	arg1	-Lex					975:978	SLex -Lex	970:978	SLex -Lex	970:978	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	2	50	theme	sperm	454:458	arg1	binding					460:466	sperm binding	454:466	sperm binding	454:466	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	7	51	theme	monovalent	1169:1178	arg1	counterparts					1180:1191	their monovalent counterparts	1163:1191	their monovalent counterparts	1163:1191	In addition, trivalent SLex -Lex and SLex were prepared which showed greater inhibitory activity compared to their monovalent counterparts.					
29603480	4	52	theme	human	798:802	arg1	sperm					804:808	human sperm	798:808	human sperm	798:808	The compounds were examined for their ability to inhibit the interaction of human sperm to ZP.					
29603480	6	53	theme	potent	1036:1041	arg1	inhibitor					1043:1051	the more potent inhibitor	1027:1051	the more potent inhibitor	1027:1051	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	6	53	theme	potent	1036:1041	arg1	structure					1014:1022	the extended structure	1001:1022	the extended structure	1001:1022	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	1	54	theme	Complex	140:146	arg1	N-glycans					148:156	Complex N-glycans	140:156	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes	140:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	7	55	theme	SLex	1077:1080	arg1	-Lex					1082:1085	trivalent SLex -Lex	1067:1085	trivalent SLex -Lex	1067:1085	In addition, trivalent SLex -Lex and SLex were prepared which showed greater inhibitory activity compared to their monovalent counterparts.					
29603480	3	56	theme	-Lex	653:656	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	56	theme	-Lex	653:656	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	4	57	theme	sperm	804:808	arg1	interaction					783:793	the interaction	779:793	the interaction of human sperm to ZP	779:814	The compounds were examined for their ability to inhibit the interaction of human sperm to ZP.					
29603480	9	58	theme	glycosylation	1571:1583	arg1	ZP					1594:1595	ZP	1594:1595	ZP	1594:1595	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	58	theme	glycosylation	1571:1583	arg1	sites					1585:1589	the various glycosylation sites	1559:1589	the various glycosylation sites of ZP that accounts for high avidity binding	1559:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	2	59	theme	N-glycans	331:339	arg1	termini					275:281	The termini	271:281	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans	271:339	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	9	60	theme	-Lex	1551:1554	arg1	presentation					1521:1532	the presentation	1517:1532	the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding	1517:1634	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	5	61	theme	minimal	933:939	arg1	effect					941:946	minimal effect	933:946	minimal effect	933:946	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	2	62	theme	tetra-antennary	315:329	arg1	N-glycans					331:339	tetra-antennary N-glycans	315:339	tetra-antennary N-glycans	315:339	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	3	63	theme	triantennary	533:544	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	64	theme	N-glycans	546:554	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	64	theme	N-glycans	546:554	arg1	synthesis					505:513	the chemoenzymatic synthesis	486:513	the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	486:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	64	theme	N-glycans	546:554	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	1	65	theme	glycoproteins	161:173	arg1	N-glycans					148:156	Complex N-glycans	140:156	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes	140:217	Complex N-glycans of glycoproteins of the zona pellucida (ZP) of human oocytes have been implicated in the binding of spermatozoa.					
29603480	8	66	from	epitope	1286:1292	arg1	context					1301:1307	the context of a complex N-glycan results in a loss of inhibitory potential	1297:1371	the context of a complex N-glycan results in a loss of inhibitory potential	1297:1371	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	6	67	theme	SLex	970:973	arg1	-Lex					975:978	SLex -Lex	970:978	SLex -Lex	970:978	Further studies with SLex -Lex and SLex showed that the extended structure is the more potent inhibitor.					
29603480	3	68	theme	SLex	648:651	arg1	N-glycans					546:554	highly complex triantennary N-glycans	518:554	highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm	518:617	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	3	68	theme	SLex	648:651	arg1	residue					660:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue	623:666	a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues	623:719	We describe here the chemoenzymatic synthesis of highly complex triantennary N-glycans derived from ZP carrying SLex moieties at the C-2 and C-2' arm and a sialyl-Lewisx -Lewisx (SLex -Lex ) residue at the C-6 antenna and two closely related analogues.					
29603480	8	69	theme	potential	1363:1371	arg1	loss					1344:1347	a loss	1342:1347	a loss of inhibitory potential	1342:1371	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	2	70	theme	sialyl-Lewisx	372:384	arg1	epitope					442:448	the minimal epitope	430:448	the minimal epitope for sperm binding	430:466	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	2	70	theme	sialyl-Lewisx	372:384	arg1	tetrasaccharide					356:370	the tetrasaccharide sialyl-Lewisx	352:384	the tetrasaccharide sialyl-Lewisx (SLex )	352:392	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	2	70	theme	sialyl-Lewisx	372:384	arg1	SLex					387:390	SLex	387:390	SLex	387:390	The termini of these unusual bi-, tri-, and tetra-antennary N-glycans consist of the tetrasaccharide sialyl-Lewisx (SLex ), which was previously identified as the minimal epitope for sperm binding.					
29603480	9	71	theme	multivalent	1457:1467	arg1	display					1469:1475	the multivalent display	1453:1475	the multivalent display of SLex on a multi-antennary glycan	1453:1511	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	9	71	theme	multivalent	1457:1467	arg1	It					1443:1444	It	1443:1444	It	1443:1444	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
29603480	0	72	theme	Glycan-Mediated	78:92	arg1	Interactions					94:105	Glycan-Mediated Interactions	78:105	Glycan-Mediated Interactions between Human Sperm and Oocytes	78:137	Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N-Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes.					
29603480	8	73	theme	inhibitory	1352:1361	arg1	potential					1363:1371	inhibitory potential	1352:1371	inhibitory potential	1352:1371	Our studies show that although SLex can inhibit the binding of spermatozoa, presenting this epitope in the context of a complex N-glycan results in a loss of inhibitory potential, and in this context only SLex -Lex can make productive interactions.					
29603480	5	74	theme	SLex	839:842	arg1	critical					859:866	critical	859:866	critical	859:866	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	5	74	theme	SLex	839:842	arg1	moiety					849:854	the SLex -Lex moiety	835:854	the SLex -Lex moiety	835:854	It was found that the SLex -Lex moiety is critical for inhibitory activity, whereas the other SLex moieties exerted minimal effect.					
29603480	9	75	from	display	1469:1475	arg1	glycan					1506:1511	a multi-antennary glycan	1488:1511	a multi-antennary glycan	1488:1511	It is not the multivalent display of SLex on a multi-antennary glycan but the presentation of multiple SLex -Lex on the various glycosylation sites of ZP that accounts for high avidity binding.					
30111774	8	0	theme	cases	1200:1204	arg1	cases					1200:1204	cases	1200:1204	cases of triple-negative breast cancers	1200:1238	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	8	0	theme	cases	1200:1204	arg1	%					1195:1195	20%	1193:1195	20% of cases of triple-negative breast cancers	1193:1238	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	8	1	from	application	1252:1262	arg1	diseases					1267:1274	diseases	1267:1274	diseases with unmet clinical needs	1267:1300	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	8	2	with	diseases	1267:1274	arg1	clinical					1287:1294	clinical	1287:1294	clinical	1287:1294	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	10	3	theme	binding	1581:1587	arg1	specificity					1589:1599	a unique binding specificity	1572:1599	a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications	1572:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	1	4	theme	STn	259:261	arg1	antigen					264:270	the sialyl-Tn (STn) antigen	244:270	the sialyl-Tn (STn) antigen	244:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	9	5	theme	glycan	1352:1357	arg1	microarrays					1359:1369	glycan microarrays	1352:1369	glycan microarrays	1352:1369	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	3	6	theme	hybridoma	518:526	arg1	technology					528:537	hybridoma technology	518:537	hybridoma technology	518:537	In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma technology.					
30111774	9	7	theme	2-6-linked	1472:1481	arg1	probes					1497:1502	2-6-linked sialyl core-1 probes	1472:1502	2-6-linked sialyl core-1 probes	1472:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	0	8	theme	sialic	106:111	arg1	acids					113:117	alpha-2-6 sialic acids	96:117	alpha-2-6 sialic acids	96:117	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	10	9	theme	diagnostic	1632:1641	arg1	development					1654:1664	cancer diagnostic and future development	1625:1664	cancer diagnostic and future development of new antibody-based therapeutic applications	1625:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	6	10	dep	bladder	987:993	arg1	tissues					1017:1023	tissues	1017:1023	tissues	1017:1023	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	0	11	theme	alpha-2-6	96:104	arg1	acids					113:117	alpha-2-6 sialic acids	96:117	alpha-2-6 sialic acids	96:117	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	6	12	theme	cancer	966:971	arg1	tissue					973:978	cancer tissue	966:978	cancer tissue	966:978	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	6	13	theme	immunohistochemistry	863:882	arg1	assays					884:889	immunohistochemistry assays	863:889	immunohistochemistry assays	863:889	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	9	14	theme	core	1423:1426	arg1	antigens					1432:1439	core STn antigens	1423:1439	core STn antigens	1423:1439	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	9	15	theme	core-1	1490:1495	arg1	probes					1497:1502	2-6-linked sialyl core-1 probes	1472:1502	2-6-linked sialyl core-1 probes	1472:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	8	16	theme	cancers	1232:1238	arg1	cases					1200:1204	cases	1200:1204	cases of triple-negative breast cancers	1200:1238	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	4	17	theme	STn-expressing	648:661	arg1	breast					663:668	STn-expressing breast	648:668	STn-expressing breast	648:668	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	4	18	theme	cell	689:692	arg1	lines					694:698	cancer cell lines	682:698	cancer cell lines	682:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	8	19	theme	breast	1225:1230	arg1	cancers					1232:1238	triple-negative breast cancers	1209:1238	triple-negative breast cancers	1209:1238	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	6	20	theme	mAb	954:956	arg1	specificity					934:944	specificity	934:944	specificity	934:944	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	6	20	theme	mAb	954:956	arg1	sensitivity					918:928	a high sensitivity	911:928	a high sensitivity	911:928	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	4	21	theme	sialylated	603:612	arg1	structures					614:623	sialylated structures	603:623	sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines	603:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	9	22	theme	additional	1445:1454	arg1	ability					1456:1462	additional ability	1445:1462	additional ability to bind 2-6-linked sialyl core-1 probes	1445:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	9	23	link	2-6-linked	1472:1481	arg1	probes					1497:1502	2-6-linked sialyl core-1 probes	1472:1502	2-6-linked sialyl core-1 probes	1472:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	2	24	theme	low	398:400	arg1	specificities					402:414	low specificities	398:414	low specificities	398:414	Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity.					
30111774	0	25	theme	monoclonal	6:15	arg1	antibody					17:24	Novel monoclonal antibody	0:24	Novel monoclonal antibody L2A5	0:29	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	6	26	theme	L2A5	949:952	arg1	mAb					954:956	L2A5 mAb	949:956	L2A5 mAb	949:956	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	7	27	theme	other	1147:1151	arg1	mAbs					1162:1165	other anti-STn mAbs	1147:1165	other anti-STn mAbs	1147:1165	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	4	28	gly	sialylated	603:612	arg1	structures					614:623	sialylated structures	603:623	sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines	603:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	4	29	theme	bladder	674:680	arg1	surface					637:643	the cell surface	628:643	the cell surface of STn-expressing breast and bladder cancer cell lines	628:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	0	30	theme	Novel	0:4	arg1	antibody					17:24	Novel monoclonal antibody	0:24	Novel monoclonal antibody L2A5	0:29	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	9	31	theme	specific	1395:1402	arg1	binding					1404:1410	a highly specific binding	1386:1410	a highly specific binding of L2A5 to core STn antigens	1386:1439	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	7	32	theme	anti-STn	1153:1160	arg1	mAbs					1162:1165	other anti-STn mAbs	1147:1165	other anti-STn mAbs	1147:1165	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	9	33	theme	sialyl	1483:1488	arg1	probes					1497:1502	2-6-linked sialyl core-1 probes	1472:1502	2-6-linked sialyl core-1 probes	1472:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	5	34	gly	O-glycosylated	778:791	arg1	MUC1					811:814	MUC1	811:814	MUC1	811:814	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	5	34	gly	O-glycosylated	778:791	arg1	proteins					793:800	tumour-associated O-glycosylated proteins	760:800	tumour-associated O-glycosylated proteins	760:800	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	5	35	theme	O-glycosylated	778:791	arg1	MUC1					811:814	MUC1	811:814	MUC1	811:814	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	5	35	theme	O-glycosylated	778:791	arg1	proteins					793:800	tumour-associated O-glycosylated proteins	760:800	tumour-associated O-glycosylated proteins	760:800	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	10	36	theme	future	1647:1652	arg1	development					1654:1664	cancer diagnostic and future development	1625:1664	cancer diagnostic and future development of new antibody-based therapeutic applications	1625:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	8	37	dep	clinical	1287:1294	arg1	needs					1296:1300	needs	1296:1300	needs	1296:1300	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	7	38	theme	L2A5	1026:1029	arg1	staining					1031:1038	L2A5 staining	1026:1038	L2A5 staining	1026:1038	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	3	39	theme	monoclonal	470:479	arg1	antibody					481:488	a novel anti-STn monoclonal antibody	453:488	a novel anti-STn monoclonal antibody named L2A5	453:499	In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma technology.					
30111774	0	40	theme	antibody	17:24	arg1	L2A5					26:29	Novel monoclonal antibody L2A5	0:29	Novel monoclonal antibody L2A5	0:29	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	10	41	theme	anti-STn	1549:1556	arg1	antibody					1558:1565	a novel anti-STn antibody	1541:1565	a novel anti-STn antibody	1541:1565	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	8	42	theme	triple-negative	1209:1223	arg1	cancers					1232:1238	triple-negative breast cancers	1209:1238	triple-negative breast cancers	1209:1238	Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs.					
30111774	1	43	theme	truncated	218:226	arg1	antigen					264:270	the sialyl-Tn (STn) antigen	244:270	the sialyl-Tn (STn) antigen	244:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	1	43	theme	truncated	218:226	arg1	glycans					228:234	truncated glycans	218:234	truncated glycans such as the sialyl-Tn (STn) antigen	218:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	10	44	theme	applications	1700:1711	arg1	development					1654:1664	cancer diagnostic and future development	1625:1664	cancer diagnostic and future development of new antibody-based therapeutic applications	1625:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	9	45	theme	L2A5	1415:1418	arg1	ability					1456:1462	additional ability	1445:1462	additional ability to bind 2-6-linked sialyl core-1 probes	1445:1502	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	9	45	theme	L2A5	1415:1418	arg1	binding					1404:1410	a highly specific binding	1386:1410	a highly specific binding of L2A5 to core STn antigens	1386:1439	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	10	46	theme	novel	1543:1547	arg1	antibody					1558:1565	a novel anti-STn antibody	1541:1565	a novel anti-STn antibody	1541:1565	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	3	47	theme	novel	455:459	arg1	antibody					481:488	a novel anti-STn monoclonal antibody	453:488	a novel anti-STn monoclonal antibody named L2A5	453:499	In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma technology.					
30111774	1	48	theme	glycans	228:234	arg1	expression					204:213	the expression	200:213	the expression of truncated glycans such as the sialyl-Tn (STn) antigen	200:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	10	49	theme	therapeutic	1688:1698	arg1	applications					1700:1711	new antibody-based therapeutic applications	1669:1711	new antibody-based therapeutic applications	1669:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	4	50	theme	cytometry	545:553	arg1	analysis					555:562	Flow cytometry analysis	540:562	Flow cytometry analysis	540:562	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	5	51	theme	tumour-associated	760:776	arg1	MUC1					811:814	MUC1	811:814	MUC1	811:814	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	5	51	theme	tumour-associated	760:776	arg1	proteins					793:800	tumour-associated O-glycosylated proteins	760:800	tumour-associated O-glycosylated proteins	760:800	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	6	52	theme	Tumour	817:822	arg1	recognition					824:834	Tumour recognition	817:834	Tumour recognition	817:834	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	3	53	theme	anti-STn	461:468	arg1	antibody					481:488	a novel anti-STn monoclonal antibody	453:488	a novel anti-STn monoclonal antibody named L2A5	453:499	In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma technology.					
30111774	10	54	theme	antibody-based	1673:1686	arg1	applications					1700:1711	new antibody-based therapeutic applications	1669:1711	new antibody-based therapeutic applications	1669:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	4	55	theme	Flow	540:543	arg1	analysis					555:562	Flow cytometry analysis	540:562	Flow cytometry analysis	540:562	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	7	56	theme	metastasis	1111:1120	arg1	sites					1122:1126	invasive and metastasis sites	1098:1126	invasive and metastasis sites	1098:1126	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	4	57	dep	breast	663:668	arg1	lines					694:698	cancer cell lines	682:698	cancer cell lines	682:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	7	58	theme	invasive	1098:1105	arg1	sites					1122:1126	invasive and metastasis sites	1098:1126	invasive and metastasis sites	1098:1126	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	1	59	theme	Incomplete	120:129	arg1	O-glycosylation					131:145	Incomplete O-glycosylation	120:145	Incomplete O-glycosylation	120:145	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	1	59	theme	Incomplete	120:129	arg1	feature					152:158	a feature	150:158	a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen	150:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	10	60	theme	new	1669:1671	arg1	applications					1700:1711	new antibody-based therapeutic applications	1669:1711	new antibody-based therapeutic applications	1669:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	6	61	theme	colorectal	999:1008	arg1	cancer					1010:1015	colorectal cancer	999:1015	colorectal cancer	999:1015	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	10	62	theme	unique	1574:1579	arg1	specificity					1589:1599	a unique binding specificity	1572:1599	a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications	1572:1711	In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.					
30111774	6	63	theme	high	913:916	arg1	sensitivity					918:928	a high sensitivity	911:928	a high sensitivity	911:928	Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues.					
30111774	7	64	from	reactivity	1084:1093	arg1	sites					1122:1126	invasive and metastasis sites	1098:1126	invasive and metastasis sites	1098:1126	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	2	65	theme	antibodies	342:351	arg1	development					305:315	the development	301:315	the development of potential anti-cancer antibodies	301:351	Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity.					
30111774	5	66	theme	immunoblotting	711:724	arg1	assays					726:731	immunoblotting assays	711:731	immunoblotting assays	711:731	Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1.					
30111774	9	67	theme	fine	1316:1319	arg1	specificity					1321:1331	the fine specificity	1312:1331	the fine specificity	1312:1331	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	7	68	theme	remarkable	1073:1082	arg1	reactivity					1084:1093	a remarkable reactivity	1071:1093	a remarkable reactivity in invasive and metastasis sites	1071:1126	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	2	69	theme	anti-cancer	330:340	arg1	antibodies					342:351	potential anti-cancer antibodies	320:351	potential anti-cancer antibodies	320:351	Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity.					
30111774	4	70	theme	cell	632:635	arg1	surface					637:643	the cell surface	628:643	the cell surface of STn-expressing breast and bladder cancer cell lines	628:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	2	71	theme	potential	320:328	arg1	antibodies					342:351	potential anti-cancer antibodies	320:351	potential anti-cancer antibodies	320:351	Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity.					
30111774	2	72	from	progress	289:296	arg1	development					305:315	the development	301:315	the development of potential anti-cancer antibodies	301:351	Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity.					
30111774	7	73	with	tumoural	1056:1063	arg1	reactivity					1084:1093	a remarkable reactivity	1071:1093	a remarkable reactivity in invasive and metastasis sites	1071:1126	L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs.					
30111774	9	74	theme	STn	1428:1430	arg1	antigens					1432:1439	core STn antigens	1423:1439	core STn antigens	1423:1439	Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2-6-linked sialyl core-1 probes.					
30111774	4	75	theme	breast	663:668	arg1	surface					637:643	the cell surface	628:643	the cell surface of STn-expressing breast and bladder cancer cell lines	628:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	4	76	theme	cancer	682:687	arg1	lines					694:698	cancer cell lines	682:698	cancer cell lines	682:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30111774	1	77	theme	sialyl-Tn	248:256	arg1	antigen					264:270	the sialyl-Tn (STn) antigen	244:270	the sialyl-Tn (STn) antigen	244:270	Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen.					
30111774	0	78	theme	short	68:72	arg1	glycans					74:80	short glycans	68:80	short glycans	68:80	Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2-6 sialic acids.					
30111774	4	79	from	structures	614:623	arg1	surface					637:643	the cell surface	628:643	the cell surface of STn-expressing breast and bladder cancer cell lines	628:698	Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines.					
30213862	6	0	theme	FcγRI	950:954	arg1	affinity					938:945	the affinity	934:945	the affinity of FcγRI / CD64, the sole "high affinity" FcγR	934:992	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	10	1	theme	400-fold	1699:1706	arg1	range					1708:1712	a 400-fold range	1697:1712	a 400-fold range in affinities	1697:1726	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	2	2	theme	particle	296:303	arg1	surface					274:280	the surface	270:280	the surface of a targeted particle	270:303	Antibodies clustered at the surface of a targeted particle trigger a protective immune response through activating FcγRs.					
30213862	3	3	theme	binding	511:517	arg1	affinity					519:526	IgG1 binding affinity	506:526	IgG1 binding affinity	506:526	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	4	4	theme	FcγRs	617:621	arg1	affinity					586:593	the affinity	582:593	the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	582:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	1	5	theme	Fc	152:153	arg1	FcγRs					168:172	FcγRs	168:172	FcγRs	168:172	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	1	5	theme	Fc	152:153	arg1	receptors					157:165	Fc γ receptors	152:165	Fc γ receptors (FcγRs)	152:173	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	10	6	theme	low	1587:1589	arg1	FcγRs					1600:1604	the low affinity FcγRs	1583:1604	the low affinity FcγRs	1583:1604	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	8	7	gly	N-glycosylation	1269:1283	arg2	sites					1285:1289	the four other N-glycosylation sites	1254:1289	the four other N-glycosylation sites	1254:1289	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	7	gly	N-glycosylation	1269:1283	arg2	four					1258:1261	four	1258:1261	four	1258:1261	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	5	8	with	CD16a	711:715	arg1	N-glycans					735:743	oligomannose N-glycans	722:743	oligomannose N-glycans	722:743	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	9	9	theme	amino	1518:1522	arg1	residues					1529:1536	only four amino acid residues	1508:1536	only four amino acid residues	1508:1536	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	4	10	theme	affinity	607:614	arg1	FcγRs					617:621	the "low affinity" FcγRs	598:621	the "low affinity" FcγRs	598:621	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	0	11	theme	crystallizable	99:112	arg1	affinity					133:140	the IgG crystallizable fragment with high affinity	91:140	the IgG crystallizable fragment with high affinity	91:140	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	6	12	theme	51-fold	820:826	arg1	increase					828:835	a 51-fold increase	818:835	a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM)	818:911	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	8	13	theme	other	1263:1267	arg1	sites					1285:1289	the four other N-glycosylation sites	1254:1289	the four other N-glycosylation sites	1254:1289	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	0	14	theme	high	128:131	arg1	affinity					133:140	the IgG crystallizable fragment with high affinity	91:140	the IgG crystallizable fragment with high affinity	91:140	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	6	15	with	CD16a	868:872	arg1	N-glycans					892:900	complex-type N-glycans	879:900	complex-type N-glycans	879:900	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	4	16	gly	N-glycoforms	651:662	arg1	Fc					648:649	six homogeneous IgG1 Fc N-glycoforms	627:662	six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	627:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	4	16	gly	N-glycoforms	651:662	arg1	IgG1					643:646	six homogeneous IgG1 Fc N-glycoforms	627:662	six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	627:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	8	17	dep	mutation	1231:1238	arg1	to					1224:1225	to	1224:1225	to	1224:1225	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	18	theme	tighter	1301:1307	arg1	binding					1309:1315	tighter binding	1301:1315	tighter binding in the Man5 glycoform	1301:1337	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	4	19	theme	IgG1	643:646	arg1	N-glycoforms					651:662	six homogeneous IgG1 Fc N-glycoforms	627:662	six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	627:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	0	20	theme	low	52:54	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	20	theme	low	52:54	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	7	21	theme	CD16a	995:999	arg1	composition					1010:1020	CD16a N-glycan composition	995:1020	CD16a N-glycan composition	995:1020	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	5	22	theme	KD=	776:778	arg1	nM					789:790	a KD= 1.0 ±0.1 nM	774:790	a KD= 1.0 ±0.1 nM	774:790	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	10	23	dep	unique	1570:1575	arg1	provides					1688:1695	provides	1688:1695	provides a 400-fold range in affinities	1688:1726	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	0	24	theme	"	64:64	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	24	theme	"	64:64	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	5	25	theme	±0.1	784:787	arg1	nM					789:790	a KD= 1.0 ±0.1 nM	774:790	a KD= 1.0 ±0.1 nM	774:790	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	8	26	theme	Man5	1324:1327	arg1	glycoform					1329:1337	the Man5 glycoform	1320:1337	the Man5 glycoform	1320:1337	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	0	27	theme	γ	69:69	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	27	theme	γ	69:69	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	9	28	theme	low	1358:1360	arg1	FcγRs					1371:1375	the other low affinity FcγRs	1348:1375	the other low affinity FcγRs	1348:1375	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	8	29	gly	glycoform	1329:1337	arg1	Man5					1324:1327	the Man5 glycoform	1320:1337	the Man5 glycoform	1320:1337	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	30	from	sites	1285:1289	arg1	mutations					1241:1249	mutations	1241:1249	mutations at the four other N-glycosylation sites	1241:1289	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	9	31	theme	FcγRs	1371:1375	arg1	None					1340:1343	None	1340:1343	None of the other low affinity FcγRs	1340:1375	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	6	32	dep	FcγRI	950:954	arg1	CD64					958:961	CD64	958:961	CD64	958:961	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	0	33	theme	oligomannose-type	11:27	arg1	N-glycans					29:37	oligomannose-type N-glycans	11:37	oligomannose-type N-glycans	11:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	1	34	theme	leukocytes	234:243	arg1	surface					223:229	the surface	219:229	the surface of leukocytes	219:243	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	10	35	theme	IgG1	1660:1663	arg1	Fc					1665:1666	the IgG1 Fc	1656:1666	the IgG1 Fc ligand	1656:1673	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	8	36	theme	Asn	1220:1222	arg1	mutation					1231:1238	an Asn to Gln mutation	1217:1238	an Asn to Gln mutation	1217:1238	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	37	gly	glycosylation	1190:1202	arg2	N162					1207:1210	N162	1207:1210	N162	1207:1210	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	37	gly	glycosylation	1190:1202	arg1	N162					1207:1210	N162	1207:1210	N162	1207:1210	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	8	38	theme	Gln	1227:1229	arg1	mutation					1231:1238	an Asn to Gln mutation	1217:1238	an Asn to Gln mutation	1217:1238	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	2	39	theme	targeted	287:294	arg1	particle					296:303	a targeted particle	285:303	a targeted particle	285:303	Antibodies clustered at the surface of a targeted particle trigger a protective immune response through activating FcγRs.					
30213862	0	40	theme	only	46:49	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	40	theme	only	46:49	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	6	41	theme	sole	968:971	arg1	FcγR					989:992	the sole "high affinity" FcγR	964:992	the sole "high affinity" FcγR	964:992	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	6	41	theme	sole	968:971	arg1	FcγRI					950:954	FcγRI	950:954	FcγRI	950:954	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	10	42	theme	receptor	1679:1686	arg1	composition					1636:1646	only the glycan composition	1620:1646	only the glycan composition of both the IgG1 Fc ligand and receptor	1620:1686	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	0	43	with	CD16a	0:4	arg1	N-glycans					29:37	oligomannose-type N-glycans	11:37	oligomannose-type N-glycans	11:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	3	44	theme	carbohydrate	445:456	arg1	N-glycans					466:474	N-glycans	466:474	N-glycans	466:474	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	3	44	theme	carbohydrate	445:456	arg1	chains					458:463	the asparagine-linked carbohydrate chains	423:463	the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a	423:495	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	1	45	theme	circulating	180:190	arg1	G					207:207	circulating immunoglobulin G	180:207	circulating immunoglobulin G (IgG1)	180:214	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	1	45	theme	circulating	180:190	arg1	IgG1					210:213	IgG1	210:213	IgG1	210:213	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	9	46	theme	receptor	1433:1440	arg1	composition					1451:1461	the receptor N-glycan composition	1429:1461	the receptor N-glycan composition	1429:1461	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	4	47	theme	homogeneous	631:641	arg1	N-glycoforms					651:662	six homogeneous IgG1 Fc N-glycoforms	627:662	six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	627:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	10	48	theme	ligand	1668:1673	arg1	composition					1636:1646	only the glycan composition	1620:1646	only the glycan composition of both the IgG1 Fc ligand and receptor	1620:1686	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	3	49	theme	FcγRIIIa	480:487	arg1	N-glycans					466:474	N-glycans	466:474	N-glycans	466:474	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	3	49	theme	FcγRIIIa	480:487	arg1	chains					458:463	the asparagine-linked carbohydrate chains	423:463	the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a	423:495	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	7	50	theme	binding	1049:1055	arg1	affinity					1057:1064	binding affinity	1049:1064	binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold)	1049:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	8	51	from	N162	1207:1210	arg1	glycosylation					1190:1202	glycosylation	1190:1202	glycosylation at N162 with an Asn to Gln mutation	1190:1238	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	7	52	theme	IgG1	1080:1083	arg1	50-fold					1113:1119	50-fold	1113:1119	50-fold	1113:1119	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	52	theme	IgG1	1080:1083	arg1	glycoforms					1088:1097	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	3	53	theme	IgG1	506:509	arg1	affinity					519:526	IgG1 binding affinity	506:526	IgG1 binding affinity	506:526	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	3	54	link	asparagine-linked	427:443	arg1	N-glycans					466:474	N-glycans	466:474	N-glycans	466:474	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	3	54	link	asparagine-linked	427:443	arg1	chains					458:463	the asparagine-linked carbohydrate chains	423:463	the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a	423:495	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	4	55	theme	N-glycan	552:559	arg1	composition					561:571	N-glycan composition	552:571	N-glycan composition	552:571	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	4	56	theme	"	615:615	arg1	FcγRs					617:621	the "low affinity" FcγRs	598:621	the "low affinity" FcγRs	598:621	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	10	57	theme	affinity	1591:1598	arg1	FcγRs					1600:1604	the low affinity FcγRs	1583:1604	the low affinity FcγRs	1583:1604	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	1	58	theme	γ	155:155	arg1	FcγRs					168:172	FcγRs	168:172	FcγRs	168:172	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	1	58	theme	γ	155:155	arg1	receptors					157:165	Fc γ receptors	152:165	Fc γ receptors (FcγRs)	152:173	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	6	59	theme	"	987:987	arg1	FcγR					989:992	the sole "high affinity" FcγR	964:992	the sole "high affinity" FcγR	964:992	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	6	59	theme	"	987:987	arg1	FcγRI					950:954	FcγRI	950:954	FcγRI	950:954	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	10	60	from	range	1708:1712	arg1	affinities					1717:1726	affinities	1717:1726	affinities	1717:1726	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	2	61	theme	protective	315:324	arg1	response					333:340	a protective immune response	313:340	a protective immune response	313:340	Antibodies clustered at the surface of a targeted particle trigger a protective immune response through activating FcγRs.					
30213862	6	62	theme	high	974:977	arg1	FcγR					989:992	the sole "high affinity" FcγR	964:992	the sole "high affinity" FcγR	964:992	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	6	62	theme	high	974:977	arg1	FcγRI					950:954	FcγRI	950:954	FcγRI	950:954	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	0	63	theme	fragment with	114:126	arg1	affinity					133:140	the IgG crystallizable fragment with high affinity	91:140	the IgG crystallizable fragment with high affinity	91:140	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	5	64	theme	IgG1	754:757	arg1	A2G2					763:766	A2G2	763:766	A2G2	763:766	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	5	64	theme	IgG1	754:757	arg1	Fc					759:760	IgG1 Fc	754:760	IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM	754:790	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	9	65	theme	acid	1524:1527	arg1	residues					1529:1536	only four amino acid residues	1508:1536	only four amino acid residues	1508:1536	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	8	66	theme	N-glycosylation	1269:1283	arg1	sites					1285:1289	the four other N-glycosylation sites	1254:1289	the four other N-glycosylation sites	1254:1289	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	4	67	theme	low	603:605	arg1	FcγRs					617:621	the "low affinity" FcγRs	598:621	the "low affinity" FcγRs	598:621	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	6	68	theme	affinity	979:986	arg1	FcγR					989:992	the sole "high affinity" FcγR	964:992	the sole "high affinity" FcγR	964:992	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	6	68	theme	affinity	979:986	arg1	FcγRI					950:954	FcγRI	950:954	FcγRI	950:954	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	9	69	theme	other	1352:1356	arg1	FcγRs					1371:1375	the other low affinity FcγRs	1348:1375	the other low affinity FcγRs	1348:1375	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	0	70	theme	affinity	56:63	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	70	theme	affinity	56:63	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	10	71	theme	glycan	1629:1634	arg1	composition					1636:1646	only the glycan composition	1620:1646	only the glycan composition of both the IgG1 Fc ligand and receptor	1620:1686	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	8	72	theme	remarkable	1128:1137	arg1	sensitivity					1139:1149	This remarkable sensitivity	1123:1149	This remarkable sensitivity	1123:1149	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	4	73	dep	N-glycoforms	651:662	arg1	G0					665:666	G0	665:666	G0	665:666	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	4	73	dep	N-glycoforms	651:662	arg1	G0F					669:671	G0F	669:671	G0F	669:671	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	4	73	dep	N-glycoforms	651:662	arg1	G2					674:675	G2	674:675	G2	674:675	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	4	73	dep	N-glycoforms	651:662	arg1	A2G2F					689:693	A2G2F	689:693	A2G2F	689:693	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	0	74	theme	Fc	66:67	arg1	receptor					71:78	the only "low affinity" Fc γ receptor	42:78	the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro	42:149	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	0	74	theme	Fc	66:67	arg1	CD16a					0:4	CD16a	0:4	CD16a with oligomannose-type N-glycans	0:37	CD16a with oligomannose-type N-glycans is the only "low affinity" Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro.					
30213862	6	75	theme	complex-type	879:890	arg1	N-glycans					892:900	complex-type N-glycans	879:900	complex-type N-glycans	879:900	This affinity represents a 51-fold increase over the affinity measured for CD16a with complex-type N-glycans (51 ±8 nM) and is comparable to the affinity of FcγRI / CD64, the sole "high affinity" FcγR.					
30213862	4	76	theme	Fc	648:649	arg1	N-glycoforms					651:662	six homogeneous IgG1 Fc N-glycoforms	627:662	six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F)	627:694	Here we determined how N-glycan composition affected the affinity of the "low affinity" FcγRs for six homogeneous IgG1 Fc N-glycoforms (G0, G0F, G2, G2F, A2G2, A2G2F).					
30213862	8	77	from	binding	1309:1315	arg1	glycoform					1329:1337	the Man5 glycoform	1320:1337	the Man5 glycoform	1320:1337	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	7	78	dep	tested	1099:1104	arg1	50-fold					1113:1119	50-fold	1113:1119	50-fold	1113:1119	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	78	dep	tested	1099:1104	arg1	glycoforms					1088:1097	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	8	79	with	glycosylation	1190:1202	arg1	mutation					1231:1238	an Asn to Gln mutation	1217:1238	an Asn to Gln mutation	1217:1238	This remarkable sensitivity could only be eliminated by preventing glycosylation at N162 with an Asn to Gln mutation; mutations at the four other N-glycosylation sites preserved tighter binding in the Man5 glycoform.					
30213862	9	80	theme	affinity	1362:1369	arg1	FcγRs					1371:1375	the other low affinity FcγRs	1348:1375	the other low affinity FcγRs	1348:1375	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	7	81	theme	N-glycan	1001:1008	arg1	composition					1010:1020	CD16a N-glycan composition	995:1020	CD16a N-glycan composition	995:1020	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	9	82	dep	more	1384:1387	arg1	increase					1405:1412	a 3.1-fold increase	1394:1412	more than a 3.1-fold increase	1384:1412	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	2	83	theme	immune	326:331	arg1	response					333:340	a protective immune response	313:340	a protective immune response	313:340	Antibodies clustered at the surface of a targeted particle trigger a protective immune response through activating FcγRs.					
30213862	5	84	theme	oligomannose	722:733	arg1	N-glycans					735:743	oligomannose N-glycans	722:743	oligomannose N-glycans	722:743	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	7	85	gly	glycoforms	1088:1097	arg1	Fc					1085:1086	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	85	gly	glycoforms	1088:1097	arg1	IgG1					1080:1083	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	5	86	with	Fc	759:760	arg1	nM					789:790	a KD= 1.0 ±0.1 nM	774:790	a KD= 1.0 ±0.1 nM	774:790	Surprisingly, CD16a with oligomannose N-glycans bound to IgG1 Fc (A2G2) with a KD= 1.0 ±0.1 nM.					
30213862	10	87	theme	Fc	1665:1666	arg1	ligand					1668:1673	the IgG1 Fc ligand	1656:1673	the IgG1 Fc ligand	1656:1673	This result indicates CD16a is unique among the low affinity FcγRs and modifying only the glycan composition of both the IgG1 Fc ligand and receptor provides a 400-fold range in affinities.					
30213862	3	88	theme	asparagine-linked	427:443	arg1	N-glycans					466:474	N-glycans	466:474	N-glycans	466:474	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	3	88	theme	asparagine-linked	427:443	arg1	chains					458:463	the asparagine-linked carbohydrate chains	423:463	the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a	423:495	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	9	89	theme	3.1-fold	1396:1403	arg1	increase					1405:1412	a 3.1-fold increase	1394:1412	more than a 3.1-fold increase	1384:1412	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	3	90	theme	recent	374:379	arg1	reports					381:387	Three recent reports	368:387	Three recent reports	368:387	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	7	91	from	increases	1036:1044	arg1	affinity					1057:1064	binding affinity	1049:1064	binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold)	1049:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	92	dep	50-fold	1113:1119	arg1	to					1110:1111	to	1110:1111	to	1110:1111	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	3	93	theme	chains	458:463	arg1	composition					408:418	the composition	404:418	the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a	404:495	Three recent reports indicated that the composition of the asparagine-linked carbohydrate chains (N-glycans) of FcγRIIIa / CD16a impacted IgG1 binding affinity.					
30213862	1	94	theme	immunoglobulin	192:205	arg1	G					207:207	circulating immunoglobulin G	180:207	circulating immunoglobulin G (IgG1)	180:214	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	1	94	theme	immunoglobulin	192:205	arg1	IgG1					210:213	IgG1	210:213	IgG1	210:213	Fc γ receptors (FcγRs) bind circulating immunoglobulin G (IgG1) at the surface of leukocytes.					
30213862	9	95	theme	N-glycan	1442:1449	arg1	composition					1451:1461	the receptor N-glycan composition	1429:1461	the receptor N-glycan composition	1429:1461	None of the other low affinity FcγRs showed more than a 3.1-fold increase upon modifying the receptor N-glycan composition, including CD16b which differs from CD16a by only four amino acid residues.					
30213862	7	96	theme	Fc	1085:1086	arg1	50-fold					1113:1119	50-fold	1113:1119	50-fold	1113:1119	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	96	theme	Fc	1085:1086	arg1	glycoforms					1088:1097	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	97	theme	other	1074:1078	arg1	50-fold					1113:1119	50-fold	1113:1119	50-fold	1113:1119	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
30213862	7	97	theme	other	1074:1078	arg1	glycoforms					1088:1097	the other IgG1 Fc glycoforms	1070:1097	the other IgG1 Fc glycoforms tested (10 to 50-fold)	1070:1120	CD16a N-glycan composition accounted for increases in binding affinity for the other IgG1 Fc glycoforms tested (10 to 50-fold).					
29562594	6	0	theme	treated	1186:1192	arg1	plants					1194:1199	kifunensine treated plants	1174:1199	kifunensine treated plants	1174:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	3	1	theme	capillary	581:589	arg1	CMG2-Fc					616:622	CMG2-Fc	616:622	CMG2-Fc	616:622	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	1	theme	capillary	581:589	arg1	protein					745:751	a model protein	737:751	a model protein	737:751	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	1	theme	capillary	581:589	arg1	protein					605:611	Fc-fused capillary morphogenesis protein 2	572:613	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain	572:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	6	2	theme	mg/kg	1279:1283	arg1	FW					1290:1291	717 mg/kg leaf FW	1275:1291	717 mg/kg leaf FW	1275:1291	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	7	3	theme	viral	1487:1491	arg1	vaccine					1493:1499	HIV-1 viral vaccine	1481:1499	HIV-1 viral vaccine	1481:1499	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	8	4	theme	small	1714:1718	arg1	inhibitors					1729:1738	other small molecule inhibitors	1708:1738	other small molecule inhibitors of glycan-processing enzymes	1708:1767	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	6	5	theme	untreated	1306:1314	arg1	plants					1316:1321	untreated plants	1306:1321	untreated plants	1306:1321	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	1	6	theme	oligomannose-type	298:314	arg1	glycans					316:322	oligomannose-type glycans	298:322	oligomannose-type glycans	298:322	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	7	7	theme	antibodies	1524:1533	arg1	production					1467:1476	the production	1463:1476	the production of HIV-1 viral vaccine and certain monoclonal antibodies	1463:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	4	8	theme	agroinfiltration	904:919	arg1	suspension					921:930	the agroinfiltration suspension	900:930	the agroinfiltration suspension	900:930	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	9	9	from	purity	1990:1995	arg1	AWF					1921:1923	AWF	1921:1923	AWF	1921:1923	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	9	from	purity	1990:1995	arg1	fluid					1914:1918	apoplast wash fluid	1900:1918	apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity	1900:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	9	from	purity	1990:1995	arg1	concentration					2001:2013	concentration	2001:2013	concentration	2001:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	7	10	theme	in	1361:1362	arg1	modification					1380:1391	in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1361:1533	in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1361:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	5	11	theme	CMG2-Fc	937:943	arg1	profile					954:960	The CMG2-Fc N-glycan profile	933:960	The CMG2-Fc N-glycan profile	933:960	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	7	12	theme	certain	1505:1511	arg1	antibodies					1524:1533	certain monoclonal antibodies	1505:1533	certain monoclonal antibodies	1505:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	2	13	from	infiltration	383:394	arg1	tissue					420:425	plant tissue	414:425	plant tissue	414:425	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	2	14	theme	first	343:347	arg1	time					349:352	the first time	339:352	the first time	339:352	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	4	15	theme	fusion	766:771	arg1	protein					773:779	The CMG2-Fc fusion protein	754:779	The CMG2-Fc fusion protein	754:779	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	9	16	theme	downstream	2064:2073	arg1	processing					2075:2084	the downstream processing	2060:2084	the downstream processing	2060:2084	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	1	17	theme	mannose	245:251	arg1	residues					253:260	trimming mannose residues	236:260	trimming mannose residues on glycoproteins	236:277	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	8	18	theme	protein	1628:1634	arg1	yield					1636:1640	protein yield	1628:1640	protein yield	1628:1640	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	7	19	theme	N-glycan	1420:1427	arg1	structures					1429:1438	more human-like N-glycan structures	1404:1438	more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1404:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	1	20	theme	potent	147:152	arg1	Kifunensine					132:142	Kifunensine	132:142	Kifunensine	132:142	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	1	20	theme	potent	147:152	arg1	inhibitor					168:176	a potent and selective inhibitor	145:176	a potent and selective inhibitor of class I α-mannosidases	145:202	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	3	21	theme	Nicotiana	691:699	arg1	plants					719:724	Nicotiana benthamiana whole plants	691:724	Nicotiana benthamiana whole plants	691:724	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	4	22	from	concentration	873:885	arg1	suspension					921:930	the agroinfiltration suspension	900:930	the agroinfiltration suspension	900:930	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	4	22	from	concentration	873:885	arg1	kifunensine					856:866	kifunensine	856:866	kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension	856:930	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	5	23	theme	dynamic	1008:1014	arg1	monitoring					1034:1043	dynamic multiple reaction monitoring	1008:1043	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	5	23	theme	dynamic	1008:1014	arg1	MRM					1046:1048	MRM	1046:1048	MRM	1046:1048	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	6	24	theme	infiltrated	1095:1105	arg1	tissue					1113:1118	the infiltrated plant tissue	1091:1118	the infiltrated plant tissue	1091:1118	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	3	25	from	site	656:659	arg1	domain					671:676	the Fc domain	664:676	the Fc domain	664:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	9	26	theme	glycoform-modified	1861:1878	arg1	protein					1887:1893	glycoform-modified target protein	1861:1893	glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1861:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	8	27	theme	glycan	1555:1560	arg1	modifications					1562:1574	glycan modifications	1555:1574	glycan modifications using a bioprocessing approach	1555:1605	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	4	28	theme	CMG2-Fc	758:764	arg1	protein					773:779	The CMG2-Fc fusion protein	754:779	The CMG2-Fc fusion protein	754:779	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	0	29	theme	Transient	97:105	arg1	Agroinfiltration					114:129	Transient Vacuum Agroinfiltration	97:129	Transient Vacuum Agroinfiltration	97:129	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	1	30	theme	I	187:187	arg1	α-mannosidases					189:202	class I α-mannosidases	181:202	class I α-mannosidases	181:202	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	6	31	theme	expression	1071:1080	arg1	weight					1226:1231	874 mg/kg leaf fresh weight	1205:1231	874 mg/kg leaf fresh weight (FW)	1205:1236	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	31	theme	expression	1071:1080	arg1	level					1082:1086	The CMG2-Fc expression level	1059:1086	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants	1059:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	9	32	theme	protein	1887:1893	arg1	collection					1847:1856	collection	1847:1856	collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1847:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	2	33	gly	glycosylation	437:449	arg1	protein					468:474	a recombinant protein	454:474	a recombinant protein transiently produced in whole-plants	454:511	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	1	34	theme	selective	158:166	arg1	Kifunensine					132:142	Kifunensine	132:142	Kifunensine	132:142	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	1	34	theme	selective	158:166	arg1	inhibitor					168:176	a potent and selective inhibitor	145:176	a potent and selective inhibitor of class I α-mannosidases	145:202	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	9	35	theme	apoplast	1900:1907	arg1	AWF					1921:1923	AWF	1921:1923	AWF	1921:1923	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	35	theme	apoplast	1900:1907	arg1	fluid					1914:1918	apoplast wash fluid	1900:1918	apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity	1900:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	8	36	theme	bioprocessing	1584:1596	arg1	approach					1598:1605	a bioprocessing approach	1582:1605	a bioprocessing approach	1582:1605	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	2	37	theme	N-linked	428:435	arg1	glycosylation					437:449	N-linked glycosylation	428:449	N-linked glycosylation of a recombinant protein transiently produced in whole-plants	428:511	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	6	38	theme	N-glycans	1160:1168	arg1	tissue					1113:1118	the infiltrated plant tissue	1091:1118	the infiltrated plant tissue	1091:1118	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	38	theme	N-glycans	1160:1168	arg1	percentage					1128:1137	the percentage	1124:1137	the percentage of oligomannose-type N-glycans	1124:1168	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	39	from	level	1082:1086	arg1	tissue					1113:1118	the infiltrated plant tissue	1091:1118	the infiltrated plant tissue	1091:1118	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	39	from	level	1082:1086	arg1	percentage					1128:1137	the percentage	1124:1137	the percentage of oligomannose-type N-glycans	1124:1168	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	5	40	theme	reaction	1025:1032	arg1	monitoring					1034:1043	dynamic multiple reaction monitoring	1008:1043	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	5	40	theme	reaction	1025:1032	arg1	MRM					1046:1048	MRM	1046:1048	MRM	1046:1048	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	3	41	contain	containing	625:634	arg2	site					656:659	one N-glycosylation site	636:659	one N-glycosylation site on the Fc domain	636:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	41	contain	containing	625:634	arg1	CMG2-Fc					616:622	CMG2-Fc	616:622	CMG2-Fc	616:622	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	41	contain	containing	625:634	arg1	protein					745:751	a model protein	737:751	a model protein	737:751	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	41	contain	containing	625:634	arg1	protein					605:611	Fc-fused capillary morphogenesis protein 2	572:613	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain	572:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	2	42	theme	recombinant	456:466	arg1	protein					468:474	a recombinant protein	454:474	a recombinant protein transiently produced in whole-plants	454:511	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	2	43	link	N-linked	428:435	arg1	glycosylation					437:449	N-linked glycosylation	428:449	N-linked glycosylation of a recombinant protein transiently produced in whole-plants	428:511	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	9	44	theme	whole-leaf	2023:2032	arg1	extract					2034:2040	whole-leaf extract	2023:2040	whole-leaf extract	2023:2040	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	0	45	theme	Recombinant	35:45	arg1	Glycoproteins					47:59	Secreted Recombinant Glycoproteins	26:59	Secreted Recombinant Glycoproteins	26:59	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	9	46	from	concentration	2001:2013	arg1	collection					1847:1856	collection	1847:1856	collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1847:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	46	from	concentration	2001:2013	arg1	protein					1887:1893	glycoform-modified target protein	1861:1893	glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1861:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	47	theme	plant-specific	1939:1952	arg1	N-glycan					1962:1969	minimal plant-specific complex N-glycan	1931:1969	minimal plant-specific complex N-glycan	1931:1969	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	3	48	theme	whole	713:717	arg1	plants					719:724	Nicotiana benthamiana whole plants	691:724	Nicotiana benthamiana whole plants	691:724	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	2	49	theme	vacuum	376:381	arg1	infiltration					383:394	one-time vacuum infiltration	367:394	one-time vacuum infiltration of kifunensine in plant tissue	367:425	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	2	50	dep	complex-type	537:548	arg1	oligomannose-type					553:569	oligomannose-type	553:569	oligomannose-type	553:569	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	2	50	dep	complex-type	537:548	arg1	to					550:551	to	550:551	to	550:551	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	6	51	theme	leaf	1215:1218	arg1	FW					1234:1235	FW	1234:1235	FW	1234:1235	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	51	theme	leaf	1215:1218	arg1	weight					1226:1231	874 mg/kg leaf fresh weight	1205:1231	874 mg/kg leaf fresh weight (FW)	1205:1236	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	51	theme	leaf	1215:1218	arg1	level					1082:1086	The CMG2-Fc expression level	1059:1086	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants	1059:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	8	52	theme	primary	1665:1671	arg1	sequence					1673:1680	the primary sequence	1661:1680	the primary sequence	1661:1680	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	3	53	theme	model	739:743	arg1	protein					745:751	a model protein	737:751	a model protein	737:751	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	53	theme	model	739:743	arg1	protein					605:611	Fc-fused capillary morphogenesis protein 2	572:613	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain	572:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	8	54	theme	molecule	1720:1727	arg1	inhibitors					1729:1738	other small molecule inhibitors	1708:1738	other small molecule inhibitors of glycan-processing enzymes	1708:1767	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	9	55	from	collection	1847:1856	arg1	AWF					1921:1923	AWF	1921:1923	AWF	1921:1923	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	55	from	collection	1847:1856	arg1	fluid					1914:1918	apoplast wash fluid	1900:1918	apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity	1900:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	55	from	collection	1847:1856	arg1	concentration					2001:2013	concentration	2001:2013	concentration	2001:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	56	theme	higher	1974:1979	arg1	purity					1990:1995	higher starting purity	1974:1995	higher starting purity	1974:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	3	57	theme	morphogenesis	591:603	arg1	CMG2-Fc					616:622	CMG2-Fc	616:622	CMG2-Fc	616:622	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	57	theme	morphogenesis	591:603	arg1	protein					745:751	a model protein	737:751	a model protein	737:751	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	57	theme	morphogenesis	591:603	arg1	protein					605:611	Fc-fused capillary morphogenesis protein 2	572:613	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain	572:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	58	theme	Fc-fused	572:579	arg1	CMG2-Fc					616:622	CMG2-Fc	616:622	CMG2-Fc	616:622	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	58	theme	Fc-fused	572:579	arg1	protein					745:751	a model protein	737:751	a model protein	737:751	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	58	theme	Fc-fused	572:579	arg1	protein					605:611	Fc-fused capillary morphogenesis protein 2	572:613	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain	572:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	8	59	theme	enzymes	1761:1767	arg1	inhibitors					1729:1738	other small molecule inhibitors	1708:1738	other small molecule inhibitors of glycan-processing enzymes	1708:1767	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	6	60	theme	kifunensine	1174:1184	arg1	plants					1194:1199	kifunensine treated plants	1174:1199	kifunensine treated plants	1174:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	7	61	theme	monoclonal	1513:1522	arg1	antibodies					1524:1533	certain monoclonal antibodies	1505:1533	certain monoclonal antibodies	1505:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	7	62	theme	vaccine	1493:1499	arg1	production					1467:1476	the production	1463:1476	the production of HIV-1 viral vaccine and certain monoclonal antibodies	1463:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	8	63	theme	other	1708:1712	arg1	inhibitors					1729:1738	other small molecule inhibitors	1708:1738	other small molecule inhibitors of glycan-processing enzymes	1708:1767	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	1	64	theme	α-mannosidases	189:202	arg1	Kifunensine					132:142	Kifunensine	132:142	Kifunensine	132:142	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	1	64	theme	α-mannosidases	189:202	arg1	inhibitor					168:176	a potent and selective inhibitor	145:176	a potent and selective inhibitor of class I α-mannosidases	145:202	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	9	65	from	fluid	1914:1918	arg1	collection					1847:1856	collection	1847:1856	collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1847:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	65	from	fluid	1914:1918	arg1	protein					1887:1893	glycoform-modified target protein	1861:1893	glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1861:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	7	66	theme	HIV-1	1481:1485	arg1	vaccine					1493:1499	HIV-1 viral vaccine	1481:1499	HIV-1 viral vaccine	1481:1499	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	3	67	theme	N-glycosylation	640:654	arg1	site					656:659	one N-glycosylation site	636:659	one N-glycosylation site on the Fc domain	636:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	5	68	theme	N-glycan	945:952	arg1	profile					954:960	The CMG2-Fc N-glycan profile	933:960	The CMG2-Fc N-glycan profile	933:960	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	9	69	theme	recombinant	1774:1784	arg1	protein					1786:1792	recombinant protein	1774:1792	recombinant protein targeted for secretion	1774:1815	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	70	with	fluid	1914:1918	arg1	N-glycan					1962:1969	minimal plant-specific complex N-glycan	1931:1969	minimal plant-specific complex N-glycan	1931:1969	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	7	71	theme	Oligomannose	1324:1335	arg1	glycans					1337:1343	Oligomannose glycans	1324:1343	Oligomannose glycans	1324:1343	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	3	72	theme	Fc	668:669	arg1	domain					671:676	the Fc domain	664:676	the Fc domain	664:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	1	73	theme	trimming	236:243	arg1	residues					253:260	trimming mannose residues	236:260	trimming mannose residues on glycoproteins	236:277	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	6	74	theme	leaf	1285:1288	arg1	FW					1290:1291	717 mg/kg leaf FW	1275:1291	717 mg/kg leaf FW	1275:1291	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	9	75	theme	kifunensine	1818:1828	arg1	treatment					1830:1838	kifunensine treatment	1818:1838	kifunensine treatment	1818:1838	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	6	76	theme	717	1275:1277	arg1	mg/kg					1279:1283	mg/kg	1279:1283	mg/kg	1279:1283	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	3	77	gly	N-glycosylation	640:654	arg2	site					656:659	one N-glycosylation site	636:659	one N-glycosylation site on the Fc domain	636:676	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	3	77	gly	N-glycosylation	640:654	arg2	one					636:638	one	636:638	one	636:638	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	1	78	from	residues	253:260	arg1	glycoproteins					265:277	glycoproteins	265:277	glycoproteins	265:277	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	5	79	theme	targeted	999:1006	arg1	method					1051:1056	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	6	80	theme	plant	1107:1111	arg1	tissue					1113:1118	the infiltrated plant tissue	1091:1118	the infiltrated plant tissue	1091:1118	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	7	81	theme	human-like	1409:1418	arg1	structures					1429:1438	more human-like N-glycan structures	1404:1438	more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1404:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	0	82	theme	Vacuum	107:112	arg1	Agroinfiltration					114:129	Transient Vacuum Agroinfiltration	97:129	Transient Vacuum Agroinfiltration	97:129	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	1	83	theme	class	181:185	arg1	α-mannosidases					189:202	class I α-mannosidases	181:202	class I α-mannosidases	181:202	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	9	84	theme	target	1880:1885	arg1	protein					1887:1893	glycoform-modified target protein	1861:1893	glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration	1861:2013	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	6	85	theme	CMG2-Fc	1063:1069	arg1	weight					1226:1231	874 mg/kg leaf fresh weight	1205:1231	874 mg/kg leaf fresh weight (FW)	1205:1236	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	85	theme	CMG2-Fc	1063:1069	arg1	level					1082:1086	The CMG2-Fc expression level	1059:1086	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants	1059:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	1	86	gly	glycoproteins	265:277	arg1	glycoproteins					265:277	glycoproteins	265:277	glycoproteins	265:277	Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.					
29562594	7	87	theme	enzymatic	1370:1378	arg1	modification					1380:1391	in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1361:1533	in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies	1361:1533	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	9	88	theme	wash	1909:1912	arg1	AWF					1921:1923	AWF	1921:1923	AWF	1921:1923	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	88	theme	wash	1909:1912	arg1	fluid					1914:1918	apoplast wash fluid	1900:1918	apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity	1900:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	5	89	theme	multiple	1016:1023	arg1	monitoring					1034:1043	dynamic multiple reaction monitoring	1008:1043	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	5	89	theme	multiple	1016:1023	arg1	MRM					1046:1048	MRM	1046:1048	MRM	1046:1048	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	6	90	theme	oligomannose-type	1142:1158	arg1	N-glycans					1160:1168	oligomannose-type N-glycans	1142:1168	oligomannose-type N-glycans	1142:1168	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	2	91	theme	plant	414:418	arg1	tissue					420:425	plant tissue	414:425	plant tissue	414:425	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	0	92	theme	Secreted	26:33	arg1	Glycoproteins					47:59	Secreted Recombinant Glycoproteins	26:59	Secreted Recombinant Glycoproteins	26:59	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	5	93	theme	monitoring	1034:1043	arg1	method					1051:1056	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	a targeted dynamic multiple reaction monitoring (MRM) method	997:1056	The CMG2-Fc N-glycan profile was determined using LC-MS/MS with a targeted dynamic multiple reaction monitoring (MRM) method.					
29562594	2	94	theme	protein	468:474	arg1	glycosylation					437:449	N-linked glycosylation	428:449	N-linked glycosylation of a recombinant protein transiently produced in whole-plants	428:511	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	4	95	theme	vacuum	814:819	arg1	agroinfiltration					821:836	vacuum agroinfiltration	814:836	vacuum agroinfiltration	814:836	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	0	96	theme	Glycoproteins	47:59	arg1	Modification					10:21	Modification	10:21	Modification of Secreted Recombinant Glycoproteins	10:59	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	7	97	dep	in	1361:1362	arg1	vitro					1364:1368	vitro	1364:1368	vitro	1364:1368	Oligomannose glycans are amenable to in vitro enzymatic modification to produce more human-like N-glycan structures that are preferred for the production of HIV-1 viral vaccine and certain monoclonal antibodies.					
29562594	9	98	theme	minimal	1931:1937	arg1	N-glycan					1962:1969	minimal plant-specific complex N-glycan	1931:1969	minimal plant-specific complex N-glycan	1931:1969	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	3	99	theme	benthamiana	701:711	arg1	plants					719:724	Nicotiana benthamiana whole plants	691:724	Nicotiana benthamiana whole plants	691:724	Fc-fused capillary morphogenesis protein 2 (CMG2-Fc) containing one N-glycosylation site on the Fc domain, produced in Nicotiana benthamiana whole plants, served as a model protein.					
29562594	0	100	theme	Kifunensine	69:79	arg1	Addition					81:88	Kifunensine Addition	69:88	Kifunensine Addition	69:88	Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration.					
29562594	6	101	theme	fresh	1220:1224	arg1	FW					1234:1235	FW	1234:1235	FW	1234:1235	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	101	theme	fresh	1220:1224	arg1	weight					1226:1231	874 mg/kg leaf fresh weight	1205:1231	874 mg/kg leaf fresh weight (FW)	1205:1236	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	101	theme	fresh	1220:1224	arg1	level					1082:1086	The CMG2-Fc expression level	1059:1086	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants	1059:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	9	102	theme	complex	1954:1960	arg1	N-glycan					1962:1969	minimal plant-specific complex N-glycan	1931:1969	minimal plant-specific complex N-glycan	1931:1969	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	8	103	theme	sequence	1673:1680	arg1	modification					1645:1656	modification	1645:1656	modification of the primary sequence	1645:1680	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	8	103	theme	sequence	1673:1680	arg1	yield					1636:1640	protein yield	1628:1640	protein yield	1628:1640	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	4	104	theme	µM	894:895	arg1	concentration					873:885	a concentration	871:885	a concentration of 5.4 µM in the agroinfiltration suspension	871:930	The CMG2-Fc fusion protein was produced transiently through vacuum agroinfiltration, with and without kifunensine at a concentration of 5.4 µM in the agroinfiltration suspension.					
29562594	2	105	theme	one-time	367:374	arg1	infiltration					383:394	one-time vacuum infiltration	367:394	one-time vacuum infiltration of kifunensine in plant tissue	367:425	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29562594	6	106	theme	mg/kg	1209:1213	arg1	FW					1234:1235	FW	1234:1235	FW	1234:1235	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	106	theme	mg/kg	1209:1213	arg1	weight					1226:1231	874 mg/kg leaf fresh weight	1205:1231	874 mg/kg leaf fresh weight (FW)	1205:1236	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	6	106	theme	mg/kg	1209:1213	arg1	level					1082:1086	The CMG2-Fc expression level	1059:1086	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants	1059:1199	The CMG2-Fc expression level in the infiltrated plant tissue and the percentage of oligomannose-type N-glycans for kifunensine treated plants was 874 mg/kg leaf fresh weight (FW) and 98.2%, respectively, compared to 717 mg/kg leaf FW and 2.3% for untreated plants.					
29562594	8	107	theme	glycan-processing	1743:1759	arg1	enzymes					1761:1767	glycan-processing enzymes	1743:1767	glycan-processing enzymes	1743:1767	This method allows glycan modifications using a bioprocessing approach without compromising protein yield or modification of the primary sequence, and could be expanded to other small molecule inhibitors of glycan-processing enzymes.					
29562594	9	108	theme	starting	1981:1988	arg1	purity					1990:1995	higher starting purity	1974:1995	higher starting purity	1974:1995	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	9	109	with	concentration	2001:2013	arg1	N-glycan					1962:1969	minimal plant-specific complex N-glycan	1931:1969	minimal plant-specific complex N-glycan	1931:1969	For recombinant protein targeted for secretion, kifunensine treatment allows collection of glycoform-modified target protein from apoplast wash fluid (AWF) with minimal plant-specific complex N-glycan at higher starting purity and concentration than in whole-leaf extract, thus simplifying the downstream processing.					
29562594	2	110	theme	kifunensine	399:409	arg1	infiltration					383:394	one-time vacuum infiltration	367:394	one-time vacuum infiltration of kifunensine in plant tissue	367:425	We report for the first time that through one-time vacuum infiltration of kifunensine in plant tissue, N-linked glycosylation of a recombinant protein transiently produced in whole-plants shifted completely from complex-type to oligomannose-type.					
29898396	1	0	gly	glycoprotein	185:196	arg1	Env					199:201	Env	199:201	Env	199:201	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	1	0	gly	glycoprotein	185:196	arg1	glycoprotein					185:196	the HIV envelope glycoprotein	168:196	the HIV envelope glycoprotein (Env)	168:202	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	3	1	theme	antibody	525:532	arg1	lineage					534:540	an apex-targeting antibody lineage	507:540	an apex-targeting antibody lineage	507:540	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	5	2	from	immunogenicity	1004:1017	arg1	trimers					1034:1040	soluble Env trimers	1022:1040	soluble Env trimers	1022:1040	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	1	3	theme	HIV	172:174	arg1	Env					199:201	Env	199:201	Env	199:201	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	1	3	theme	HIV	172:174	arg1	glycoprotein					185:196	the HIV envelope glycoprotein	168:196	the HIV envelope glycoprotein (Env)	168:202	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	2	4	from	points	325:330	arg1	analyses					250:257	Longitudinal analyses	237:257	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection	237:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	4	5	theme	approach	763:770	arg1	steepens					772:779	approach steepens	763:779	approach steepens	763:779	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	2	6	theme	viruses	302:308	arg1	analyses					250:257	Longitudinal analyses	237:257	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection	237:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	5	7	theme	tuning	989:994	arg1	immunogenicity					1004:1017	tuning optimal immunogenicity	989:1017	tuning optimal immunogenicity in soluble Env trimers	989:1040	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	0	8	theme	Vaccine	102:108	arg1	Design					110:115	Vaccine Design	102:115	Vaccine Design	102:115	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	4	9	theme	complexes	635:643	arg1	Comparison					614:623	Comparison	614:623	Comparison of Ab-Env complexes at early and late time points	614:673	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	5	10	theme	Env	946:948	arg1	constructs					950:959	soluble and full-length Env constructs	922:959	soluble and full-length Env constructs	922:959	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	3	11	theme	structural	476:485	arg1	characterization					487:502	the structural characterization	472:502	the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort	472:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	2	12	theme	lineages	289:296	arg1	analyses					250:257	Longitudinal analyses	237:257	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection	237:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	4	13	from	points	668:673	arg1	Comparison					614:623	Comparison	614:623	Comparison of Ab-Env complexes at early and late time points	614:673	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	1	14	theme	envelope	176:183	arg1	Env					199:201	Env	199:201	Env	199:201	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	1	14	theme	envelope	176:183	arg1	glycoprotein					185:196	the HIV envelope glycoprotein	168:196	the HIV envelope glycoprotein (Env)	168:202	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	5	15	theme	site-specific	886:898	arg1	glycosylation					900:912	site-specific glycosylation	886:912	site-specific glycosylation	886:912	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	3	16	from	characterization	487:502	arg1	donor					582:586	a donor	580:586	a donor in the Protocol C cohort	580:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	2	17	theme	antibody	280:287	arg1	lineages					289:296	both neutralizing antibody lineages	262:296	both neutralizing antibody lineages	262:296	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	4	18	theme	late	658:661	arg1	points					668:673	early and late time points	648:673	early and late time points	648:673	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	6	19	theme	Env	1164:1166	arg1	V2-apex					1168:1174	the Env V2-apex	1160:1174	the Env V2-apex	1160:1174	These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.					
29898396	5	20	theme	full-length	934:944	arg1	constructs					950:959	soluble and full-length Env constructs	922:959	soluble and full-length Env constructs	922:959	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	6	21	theme	important	1075:1083	arg1	guideposts					1085:1094	important guideposts	1075:1094	important guideposts for design of immunogens	1075:1119	These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.					
29898396	4	22	theme	glycan	829:834	arg1	changes					836:842	glycan changes	829:842	glycan changes	829:842	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	2	23	theme	time	320:323	arg1	points					325:330	serial time points	313:330	serial time points during infection	313:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	4	24	theme	bnAb	749:752	arg1	angle					754:758	the bnAb angle	745:758	the bnAb angle of approach steepens	745:779	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	2	25	theme	co-evolutionary	387:401	arg1	contest					403:409	the co-evolutionary contest	383:409	the co-evolutionary contest between HIV and the humoral immune system	383:451	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	0	26	theme	Envelope	20:27	arg1	Co-evolution					0:11	Co-evolution	0:11	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	0:76	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	4	27	theme	CDRH3	722:726	arg1	loop					728:731	the CDRH3 loop rigidifies	718:742	the CDRH3 loop rigidifies	718:742	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	5	28	from	differences	871:881	arg1	glycosylation					900:912	site-specific glycosylation	886:912	site-specific glycosylation	886:912	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	2	29	theme	serial	313:318	arg1	points					325:330	serial time points	313:330	serial time points during infection	313:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	3	30	theme	C	604:604	arg1	cohort					606:611	the Protocol C cohort	591:611	the Protocol C cohort	591:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	2	31	theme	immune	439:444	arg1	system					446:451	the humoral immune system	427:451	the humoral immune system	427:451	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	0	32	theme	HIV	16:18	arg1	Envelope					20:27	HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	16:76	Envelope	20:27	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	3	33	dep	lineage	534:540	arg1	Env					571:573	A viral Env	563:573	A viral Env	563:573	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	34	theme	clade	557:561	arg1	characterization					487:502	the structural characterization	472:502	the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort	472:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	35	theme	lineage	534:540	arg1	characterization					487:502	the structural characterization	472:502	the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort	472:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	2	36	theme	humoral	431:437	arg1	system					446:451	the humoral immune system	427:451	the humoral immune system	427:451	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	0	37	theme	Apex-Targeting	33:46	arg1	Lineage					70:76	HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	16:76	Lineage	70:76	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	4	38	theme	antibody	700:707	arg1	lineage					709:715	the antibody lineage	696:715	the antibody lineage	696:715	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	6	39	theme	mature	1136:1141	arg1	nAb					1143:1145	mature nAb	1136:1145	mature nAb	1136:1145	These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.					
29898396	2	40	theme	Longitudinal	237:248	arg1	analyses					250:257	Longitudinal analyses	237:257	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection	237:347	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	4	41	theme	time	663:666	arg1	points					668:673	early and late time points	648:673	early and late time points	648:673	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	4	42	theme	surface	786:792	arg1	charges					794:800	surface charges	786:800	surface charges	786:800	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	4	43	theme	early	648:652	arg1	points					668:673	early and late time points	648:673	early and late time points	648:673	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	0	44	theme	Antibody	61:68	arg1	Lineage					70:76	HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	16:76	Lineage	70:76	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	5	45	theme	Env	1030:1032	arg1	trimers					1034:1040	soluble Env trimers	1022:1040	soluble Env trimers	1022:1040	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	1	46	theme	neutralizing	126:137	arg1	bnAbs					151:155	bnAbs	151:155	bnAbs	151:155	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	1	46	theme	neutralizing	126:137	arg1	antibodies					139:148	Broadly neutralizing antibodies	118:148	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env)	118:202	Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop.					
29898396	0	47	theme	Neutralizing	48:59	arg1	Lineage					70:76	HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	16:76	Lineage	70:76	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	3	48	theme	Protocol	595:602	arg1	cohort					606:611	the Protocol C cohort	591:611	the Protocol C cohort	591:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	49	theme	apex-targeting	510:523	arg1	lineage					534:540	an apex-targeting antibody lineage	507:540	an apex-targeting antibody lineage	507:540	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	4	50	theme	steepens	772:779	arg1	charges					794:800	surface charges	786:800	surface charges	786:800	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	4	50	theme	steepens	772:779	arg1	angle					754:758	the bnAb angle	745:758	the bnAb angle of approach steepens	745:779	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	4	50	theme	steepens	772:779	arg1	loop					728:731	the CDRH3 loop rigidifies	718:742	the CDRH3 loop rigidifies	718:742	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	2	51	theme	neutralizing	267:278	arg1	lineages					289:296	both neutralizing antibody lineages	262:296	both neutralizing antibody lineages	262:296	Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system.					
29898396	5	52	theme	soluble	1022:1028	arg1	trimers					1034:1040	soluble Env trimers	1022:1040	soluble Env trimers	1022:1040	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	4	53	dep	loop	728:731	arg1	rigidifies					733:742	rigidifies	733:742	rigidifies	733:742	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	3	54	theme	viral	565:569	arg1	Env					571:573	A viral Env	563:573	A viral Env	563:573	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	6	55	theme	immunogens	1110:1119	arg1	design					1100:1105	design	1100:1105	design of immunogens	1100:1119	These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.					
29898396	3	56	from	donor	582:586	arg1	lineage					534:540	an apex-targeting antibody lineage	507:540	an apex-targeting antibody lineage	507:540	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	56	from	donor	582:586	arg1	characterization					487:502	the structural characterization	472:502	the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort	472:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	56	from	donor	582:586	arg1	cohort					606:611	the Protocol C cohort	591:611	the Protocol C cohort	591:611	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	3	56	from	donor	582:586	arg1	clade					557:561	autologous clade	546:561	autologous clade	546:561	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29898396	5	57	theme	soluble	922:928	arg1	constructs					950:959	soluble and full-length Env constructs	922:959	soluble and full-length Env constructs	922:959	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	5	58	theme	optimal	996:1002	arg1	immunogenicity					1004:1017	tuning optimal immunogenicity	989:1017	tuning optimal immunogenicity in soluble Env trimers	989:1040	Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers.					
29898396	4	59	theme	Ab-Env	628:633	arg1	complexes					635:643	Ab-Env complexes	628:643	Ab-Env complexes	628:643	Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes.					
29898396	0	60	theme	Lineage	70:76	arg1	Co-evolution					0:11	Co-evolution	0:11	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage	0:76	Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.					
29898396	6	61	dep	prime	1126:1130	arg1	responses					1147:1155	responses	1147:1155	responses	1147:1155	These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.					
29898396	3	62	theme	autologous	546:555	arg1	clade					557:561	autologous clade	546:561	autologous clade	546:561	Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort.					
29681470	5	0	theme	functional	733:742	arg1	experiments					744:754	functional experiments	733:754	functional experiments	733:754	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	8	1	theme	GAG	1109:1111	arg1	attachment					1113:1122	GAG attachment	1109:1122	GAG attachment	1109:1122	These findings provide a framework for understanding the selectivity of GAG attachment.					
29681470	1	2	theme	animal	153:158	arg1	matrix					174:179	the animal extracellular matrix	149:179	the animal extracellular matrix	149:179	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	1	3	theme	extracellular	160:172	arg1	matrix					174:179	the animal extracellular matrix	149:179	the animal extracellular matrix	149:179	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	1	4	theme	immune	243:248	arg1	function					250:257	immune function	243:257	immune function	243:257	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	7	5	theme	cleft	935:939	arg1	remainder					918:926	The remainder	914:926	The remainder of the cleft	914:939	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	8	6	theme	attachment	1113:1122	arg1	selectivity					1094:1104	the selectivity	1090:1104	the selectivity of GAG attachment	1090:1122	These findings provide a framework for understanding the selectivity of GAG attachment.					
29681470	3	7	theme	selected	460:467	arg1	residues					476:483	selected serine residues	460:483	selected serine residues in the core proteins	460:504	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	1	8	theme	matrix	174:179	arg1	Proteoglycans					101:113	Proteoglycans	101:113	Proteoglycans (PGs)	101:119	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	1	8	theme	matrix	174:179	arg1	components					135:144	essential components	125:144	essential components of the animal extracellular matrix	125:179	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	0	9	theme	Glycosaminoglycan	39:55	arg1	Biosynthesis					57:68	Glycosaminoglycan Biosynthesis	39:68	Glycosaminoglycan Biosynthesis	39:68	Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1.					
29681470	6	10	theme	acceptor	860:867	arg1	serine					869:874	the acceptor serine	856:874	the acceptor serine to be followed by glycine or alanine	856:911	A constriction within the peptide binding cleft requires the acceptor serine to be followed by glycine or alanine.					
29681470	2	11	theme	PG	356:357	arg1	function					359:366	PG function	356:366	PG function	356:366	PGs are composed of a core protein and long glycosaminoglycan (GAG) chains, which often specify PG function.					
29681470	4	12	theme	various	630:636	arg1	peptides					647:654	various acceptor peptides	630:654	various acceptor peptides	630:654	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	0	13	theme	Structural	0:9	arg1	Basis					11:15	Structural Basis	0:15	Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1	0:98	Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1.					
29681470	5	14	theme	XT1	794:796	arg1	specificity					779:789	the substrate specificity	765:789	the substrate specificity of XT1	765:796	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	4	15	theme	crystal	526:532	arg1	structures					534:543	crystal structures	526:543	crystal structures of human xylosyltransferase 1 (XT1)	526:579	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	7	16	theme	acidic	1020:1025	arg1	residues					1027:1034	acidic residues	1020:1034	acidic residues	1020:1034	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	3	17	from	residues	476:483	arg1	proteins					497:504	the core proteins	488:504	the core proteins	488:504	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	2	18	theme	core	282:285	arg1	protein					287:293	a core protein	280:293	a core protein	280:293	PGs are composed of a core protein and long glycosaminoglycan (GAG) chains, which often specify PG function.					
29681470	4	19	theme	sugar	601:605	arg1	donor					607:611	the sugar donor	597:611	the sugar donor	597:611	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	4	20	theme	human	548:552	arg1	xylosyltransferase					554:571	human xylosyltransferase 1	548:573	human xylosyltransferase 1 (XT1)	548:579	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	4	20	theme	human	548:552	arg1	XT1					576:578	XT1	576:578	XT1	576:578	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	1	21	theme	cell	202:205	arg1	adhesion					207:214	cell adhesion	202:214	cell adhesion	202:214	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	5	22	theme	unique	679:684	arg1	features					698:705	unique active-site features	679:705	unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1	679:796	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	5	23	theme	active-site	686:696	arg1	features					698:705	unique active-site features	679:705	unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1	679:796	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	3	24	theme	peptide	402:408	arg1	O-xylosyltransferases					410:430	peptide O-xylosyltransferases	402:430	peptide O-xylosyltransferases	402:430	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	4	25	theme	xylosyltransferase	554:571	arg1	structures					534:543	crystal structures	526:543	crystal structures of human xylosyltransferase 1 (XT1)	526:579	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	6	26	theme	binding	833:839	arg1	cleft					841:845	the peptide binding cleft	821:845	the peptide binding cleft	821:845	A constriction within the peptide binding cleft requires the acceptor serine to be followed by glycine or alanine.					
29681470	4	27	with	complex	584:590	arg1	donor					607:611	the sugar donor	597:611	the sugar donor	597:611	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	4	27	with	complex	584:590	arg1	peptides					647:654	various acceptor peptides	630:654	various acceptor peptides	630:654	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	4	27	with	complex	584:590	arg1	UDP-xylose					614:623	UDP-xylose	614:623	UDP-xylose	614:623	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29681470	7	28	theme	sequences	975:983	arg1	sequences					975:983	sequences	975:983	sequences	975:983	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	7	28	theme	sequences	975:983	arg1	variety					964:970	a wide variety	957:970	a wide variety of sequences	957:983	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	0	29	theme	Biosynthesis	57:68	arg1	Initiation					25:34	the Initiation	21:34	the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1	21:98	Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1.					
29681470	5	30	theme	substrate	769:777	arg1	specificity					779:789	the substrate specificity	765:789	the substrate specificity of XT1	765:796	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	6	31	theme	peptide	825:831	arg1	cleft					841:845	the peptide binding cleft	821:845	the peptide binding cleft	821:845	A constriction within the peptide binding cleft requires the acceptor serine to be followed by glycine or alanine.					
29681470	3	32	theme	serine	469:474	arg1	residues					476:483	selected serine residues	460:483	selected serine residues in the core proteins	460:504	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	3	33	theme	GAG	369:371	arg1	biosynthesis					373:384	GAG biosynthesis	369:384	GAG biosynthesis	369:384	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	7	34	theme	general	997:1003	arg1	preference					1005:1014	a general preference	995:1014	a general preference for acidic residues	995:1034	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	1	35	theme	essential	125:133	arg1	Proteoglycans					101:113	Proteoglycans	101:113	Proteoglycans (PGs)	101:119	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	1	35	theme	essential	125:133	arg1	components					135:144	essential components	125:144	essential components of the animal extracellular matrix	125:179	Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function.					
29681470	2	36	theme	long	299:302	arg1	chains					328:333	long glycosaminoglycan (GAG) chains	299:333	long glycosaminoglycan (GAG) chains	299:333	PGs are composed of a core protein and long glycosaminoglycan (GAG) chains, which often specify PG function.					
29681470	2	37	theme	glycosaminoglycan	304:320	arg1	chains					328:333	long glycosaminoglycan (GAG) chains	299:333	long glycosaminoglycan (GAG) chains	299:333	PGs are composed of a core protein and long glycosaminoglycan (GAG) chains, which often specify PG function.					
29681470	0	38	theme	Human	73:77	arg1	Xylosyltransferase					79:96	Human Xylosyltransferase 1	73:98	Human Xylosyltransferase 1	73:98	Structural Basis for the Initiation of Glycosaminoglycan Biosynthesis by Human Xylosyltransferase 1.					
29681470	5	39	with	conjunction	716:726	arg1	experiments					744:754	functional experiments	733:754	functional experiments	733:754	The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1.					
29681470	7	40	theme	wide	959:962	arg1	sequences					975:983	sequences	975:983	sequences	975:983	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	7	40	theme	wide	959:962	arg1	variety					964:970	a wide variety	957:970	a wide variety of sequences	957:983	The remainder of the cleft can accommodate a wide variety of sequences, but with a general preference for acidic residues.					
29681470	3	41	theme	core	492:495	arg1	proteins					497:504	the core proteins	488:504	the core proteins	488:504	GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins.					
29681470	4	42	theme	acceptor	638:645	arg1	peptides					647:654	various acceptor peptides	630:654	various acceptor peptides	630:654	We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides.					
29992770	6	0	theme	glycan	931:936	arg1	composition					938:948	glycan composition	931:948	glycan composition	931:948	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	1	1	theme	clinical	223:230	arg1	signs					232:236	clinical signs	223:236	clinical signs	223:236	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	0	2	theme	Specific	69:76	arg1	N-Glycopeptides					78:92	Specific N-Glycopeptides	69:92	Specific N-Glycopeptides Found in Serum	69:107	Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific N-Glycopeptides Found in Serum.					
29992770	7	3	theme	tryptic	989:995	arg1	N-glycopeptides					997:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	10	4	theme	potential	1486:1494	arg1	biomarkers					1502:1511	potential serum biomarkers	1486:1511	potential serum biomarkers	1486:1511	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	9	5	contain	have	1296:1299	arg1	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	5	contain	have	1296:1299	arg2	abundances					1337:1346	statistically significant different abundances	1301:1346	statistically significant different abundances between cases and controls	1301:1373	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	8	6	theme	expression	1197:1206	arg1	levels					1208:1213	the expression levels	1193:1213	the expression levels of these glycoforms	1193:1233	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	4	7	theme	Serum	504:508	arg1	samples					510:516	Serum samples	504:516	Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls	504:610	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	5	8	theme	Quantitative	627:638	arg1	N-glycoproteomics					651:667	Quantitative label-free N-glycoproteomics	627:667	Quantitative label-free N-glycoproteomics	627:667	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	6	9	theme	web-based	812:820	arg1	GlycopeptideID					832:845	GlycopeptideID	832:845	GlycopeptideID	832:845	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	6	9	theme	web-based	812:820	arg1	software					822:829	web-based software	812:829	web-based software (GlycopeptideID)	812:846	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	6	10	theme	glycosylation	911:923	arg1	site					925:928	glycosylation site	911:928	glycosylation site	911:928	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	5	11	theme	label-free	640:649	arg1	N-glycoproteomics					651:667	Quantitative label-free N-glycoproteomics	627:667	Quantitative label-free N-glycoproteomics	627:667	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	5	12	gly	N-glycopeptides	748:762	arg2	N-glycopeptides					748:762	the statistically significantly different N-glycopeptides	706:762	the statistically significantly different N-glycopeptides	706:762	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	10	13	theme	serum	1496:1500	arg1	biomarkers					1502:1511	potential serum biomarkers	1486:1511	potential serum biomarkers	1486:1511	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	3	14	theme	preclinical	455:465	arg1	screening					472:480	preclinical OSCC screening	455:480	preclinical OSCC screening	455:480	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	11	15	theme	stage	1666:1670	arg1	modalities					1635:1644	future diagnostic modalities	1617:1644	future diagnostic modalities of OSCC as early as stage I	1617:1672	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	5	16	theme	different	738:746	arg1	N-glycopeptides					748:762	the statistically significantly different N-glycopeptides	706:762	the statistically significantly different N-glycopeptides	706:762	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	4	17	theme	EXPERIMENTAL	483:494	arg1	DESIGN					496:501	EXPERIMENTAL DESIGN	483:501	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.	483:625	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	7	18	gly	N-glycopeptides	997:1011	arg2	N-glycopeptides					997:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	6	19	theme	database	790:797	arg1	search					799:804	a database search	788:804	a database search using web-based software (GlycopeptideID)	788:846	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	2	20	theme	OSCC	244:247	arg1	incidence					249:257	Most OSCC incidence	239:257	Most OSCC incidence	239:257	Most OSCC incidence is associated with significant morbidity and poor survival.					
29992770	1	21	theme	early-stage	159:169	arg1	cavity					176:181	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	0	22	theme	Cancer	7:12	arg1	Patients					14:21	Tongue Cancer Patients	0:21	Tongue Cancer Patients	0:21	Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific N-Glycopeptides Found in Serum.					
29992770	10	23	gly	N-glycopeptides	1458:1472	arg2	N-glycopeptides					1458:1472	specific N-glycopeptides	1449:1472	specific N-glycopeptides	1449:1472	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	10	24	theme	preclinical	1523:1533	arg1	OSCC					1535:1538	preclinical OSCC	1523:1538	preclinical OSCC in patients	1523:1550	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	9	25	theme	IgG1	1255:1258	arg1	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	1	26	theme	oral	171:174	arg1	cavity					176:181	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	0	27	theme	Tongue	0:5	arg1	Patients					14:21	Tongue Cancer Patients	0:21	Tongue Cancer Patients	0:21	Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific N-Glycopeptides Found in Serum.					
29992770	7	28	gly	N-glycosylation	1046:1060	arg2	sites					1062:1066	19 unique N-glycosylation sites	1036:1066	19 unique N-glycosylation sites representing 14 glycoproteins	1036:1096	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	7	28	gly	N-glycosylation	1046:1060	arg2	19					1036:1037	19	1036:1037	19	1036:1037	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	11	29	theme	diagnostic	1624:1633	arg1	modalities					1635:1644	future diagnostic modalities	1617:1644	future diagnostic modalities of OSCC as early as stage I	1617:1672	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	9	30	theme	different	1327:1335	arg1	abundances					1337:1346	statistically significant different abundances	1301:1346	statistically significant different abundances between cases and controls	1301:1373	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	31	theme	haptoglobin	1267:1277	arg1	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	3	32	theme	mass-spectrometry	344:360	arg1	technology					367:376	mass-spectrometry (MS) technology	344:376	mass-spectrometry (MS) technology	344:376	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	10	33	dep	CONCLUSIONS	1376:1386	arg1	authors					1416:1422	The authors	1412:1422	The authors	1412:1422	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	10	33	dep	CONCLUSIONS	1376:1386	arg1	first					1432:1436	first	1432:1436	first	1432:1436	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	6	34	with	Combined	774:781	arg1	search					799:804	a database search	788:804	a database search using web-based software (GlycopeptideID)	788:846	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	7	35	theme	Thirty-eight	976:987	arg1	N-glycopeptides					997:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides	976:1011	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	2	36	theme	significant	278:288	arg1	morbidity					290:298	significant morbidity	278:298	significant morbidity	278:298	Most OSCC incidence is associated with significant morbidity and poor survival.					
29992770	6	37	gly	N-glycopeptide	873:886	arg2	N-glycopeptide					873:886	detailed N-glycopeptide information	864:898	detailed N-glycopeptide information	864:898	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	1	38	theme	squamous	183:190	arg1	OSCC					208:211	OSCC	208:211	OSCC	208:211	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	1	38	theme	squamous	183:190	arg1	carcinoma					197:205	squamous cell carcinoma	183:205	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	5	39	theme	MS/MS	688:692	arg1	analysis					694:701	MS/MS analysis	688:701	MS/MS analysis of the statistically significantly different N-glycopeptides	688:762	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	6	40	theme	N-glycopeptide	873:886	arg1	information					888:898	detailed N-glycopeptide information	864:898	detailed N-glycopeptide information	864:898	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	10	41	from	OSCC	1535:1538	arg1	patients					1543:1550	patients	1543:1550	patients	1543:1550	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	1	42	theme	cell	192:195	arg1	OSCC					208:211	OSCC	208:211	OSCC	208:211	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	1	42	theme	cell	192:195	arg1	carcinoma					197:205	squamous cell carcinoma	183:205	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	11	43	theme	OSCC	1649:1652	arg1	modalities					1635:1644	future diagnostic modalities	1617:1644	future diagnostic modalities of OSCC as early as stage I	1617:1672	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	10	44	theme	specific	1449:1456	arg1	N-glycopeptides					1458:1472	specific N-glycopeptides	1449:1472	specific N-glycopeptides	1449:1472	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	6	45	theme	detailed	864:871	arg1	information					888:898	detailed N-glycopeptide information	864:898	detailed N-glycopeptide information	864:898	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	8	46	theme	stage	1124:1128	arg1	tumors					1132:1137	stage I tumors	1124:1137	stage I tumors	1124:1137	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	3	47	theme	serum	434:438	arg1	biomarkers					440:449	serum biomarkers	434:449	serum biomarkers for preclinical OSCC screening	434:480	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	3	48	theme	specific	386:393	arg1	N-glycopeptides					395:409	specific N-glycopeptides	386:409	specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening	386:480	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	4	49	from	patients	526:533	arg1	samples					510:516	Serum samples	504:516	Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls	504:610	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	1	50	dep	cavity	176:181	arg1	OSCC					208:211	OSCC	208:211	OSCC	208:211	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	1	50	dep	cavity	176:181	arg1	carcinoma					197:205	squamous cell carcinoma	183:205	early-stage oral cavity squamous cell carcinoma (OSCC)	159:212	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	7	51	theme	N-glycosylation	1046:1060	arg1	sites					1062:1066	19 unique N-glycosylation sites	1036:1066	19 unique N-glycosylation sites representing 14 glycoproteins	1036:1096	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	8	52	theme	glycoforms	1224:1233	arg1	levels					1208:1213	the expression levels	1193:1213	the expression levels of these glycoforms	1193:1233	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	0	53	theme	Healthy	49:55	arg1	Controls					57:64	Healthy Controls	49:64	Healthy Controls	49:64	Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific N-Glycopeptides Found in Serum.					
29992770	6	54	theme	proposed	955:962	arg1	structures					964:973	proposed structures	955:973	proposed structures	955:973	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	11	55	gly	N-glycopeptides	1559:1573	arg2	candidates					1588:1597	the lead candidates	1579:1597	the lead candidates for validation	1579:1612	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	11	55	gly	N-glycopeptides	1559:1573	arg2	N-glycopeptides					1559:1573	These N-glycopeptides	1553:1573	These N-glycopeptides	1553:1573	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	9	56	gly	N-glycopeptides	1236:1250	arg1	IgG1					1255:1258	IgG1	1255:1258	IgG1	1255:1258	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	56	gly	N-glycopeptides	1236:1250	arg2	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	56	gly	N-glycopeptides	1236:1250	arg1	haptoglobin					1267:1277	haptoglobin	1267:1277	haptoglobin	1267:1277	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	56	gly	N-glycopeptides	1236:1250	arg1	transferrin					1284:1294	transferrin	1284:1294	transferrin	1284:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	56	gly	N-glycopeptides	1236:1250	arg1	IgG4					1261:1264	IgG4	1261:1264	IgG4	1261:1264	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	9	57	theme	IgG4	1261:1264	arg1	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	8	58	theme	I	1130:1130	arg1	tumors					1132:1137	stage I tumors	1124:1137	stage I tumors	1124:1137	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	9	59	theme	transferrin	1284:1294	arg1	N-glycopeptides					1236:1250	N-glycopeptides	1236:1250	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin	1236:1294	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	8	60	theme	OSCC	1099:1102	arg1	tumors					1132:1137	stage I tumors	1124:1137	stage I tumors	1124:1137	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	8	60	theme	OSCC	1099:1102	arg1	patients					1104:1111	OSCC patients	1099:1111	OSCC patients	1099:1111	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	10	61	theme	CLINICAL	1392:1399	arg1	RELEVANCE					1401:1409	CLINICAL RELEVANCE	1392:1409	CLINICAL RELEVANCE	1392:1409	CONCLUSIONS AND CLINICAL RELEVANCE: The authors are the first to suggest specific N-glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients.					
29992770	7	62	gly	glycoproteins	1084:1096	arg1	glycoproteins					1084:1096	14 glycoproteins	1081:1096	14 glycoproteins	1081:1096	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	4	63	dep	DESIGN	496:501	arg1	collected					616:624	collected	616:624	are collected	612:624	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	2	64	theme	Most	239:242	arg1	incidence					249:257	Most OSCC incidence	239:257	Most OSCC incidence	239:257	Most OSCC incidence is associated with significant morbidity and poor survival.					
29992770	7	65	theme	unique	1039:1044	arg1	sites					1062:1066	19 unique N-glycosylation sites	1036:1066	19 unique N-glycosylation sites representing 14 glycoproteins	1036:1096	Thirty-eight tryptic N-glycopeptides are identified, having 19 unique N-glycosylation sites representing 14 glycoproteins.					
29992770	3	66	theme	OSCC	467:470	arg1	screening					472:480	preclinical OSCC screening	455:480	preclinical OSCC screening	455:480	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	6	67	gly	glycosylation	911:923	arg2	site					925:928	glycosylation site	911:928	glycosylation site	911:928	RESULTS: Combined with a database search using web-based software (GlycopeptideID), MS/MS provided detailed N-glycopeptide information, including glycosylation site, glycan composition, and proposed structures.					
29992770	11	68	dep	early	1657:1661	arg1	as					1663:1664	as	1663:1664	as	1663:1664	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	2	69	theme	poor	304:307	arg1	survival					309:316	poor survival	304:316	poor survival	304:316	Most OSCC incidence is associated with significant morbidity and poor survival.					
29992770	5	70	theme	N-glycopeptides	748:762	arg1	analysis					694:701	MS/MS analysis	688:701	MS/MS analysis of the statistically significantly different N-glycopeptides	688:762	Quantitative label-free N-glycoproteomics is performed, with MS/MS analysis of the statistically significantly different N-glycopeptides.					
29992770	9	71	theme	significant	1315:1325	arg1	abundances					1337:1346	statistically significant different abundances	1301:1346	statistically significant different abundances between cases and controls	1301:1373	N-glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.					
29992770	4	72	theme	sex-matched	591:601	arg1	controls					603:610	12 age- and sex-matched controls	579:610	12 age- and sex-matched controls	579:610	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	11	73	theme	future	1617:1622	arg1	modalities					1635:1644	future diagnostic modalities	1617:1644	future diagnostic modalities of OSCC as early as stage I	1617:1672	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	4	74	theme	age-	582:585	arg1	controls					603:610	12 age- and sex-matched controls	579:610	12 age- and sex-matched controls	579:610	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	8	75	theme	healthy	1167:1173	arg1	controls					1175:1182	healthy controls	1167:1182	healthy controls	1167:1182	OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms.					
29992770	4	76	dep	treated	535:541	arg1	stage					564:568	stage IV	564:571	stage IV	564:571	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	4	76	dep	treated	535:541	arg1	patients					526:533	14 patients	523:533	14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls	523:610	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	4	76	dep	treated	535:541	arg1	stage					553:557	stage I	553:559	stage I	553:559	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29992770	3	77	gly	N-glycopeptides	395:409	arg2	N-glycopeptides					395:409	specific N-glycopeptides	386:409	specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening	386:480	The authors aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.					
29992770	1	78	theme	blood	132:136	arg1	biomarkers					138:147	no blood biomarkers	129:147	no blood biomarkers	129:147	PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs.					
29992770	11	79	theme	lead	1583:1586	arg1	N-glycopeptides					1559:1573	These N-glycopeptides	1553:1573	These N-glycopeptides	1553:1573	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	11	79	theme	lead	1583:1586	arg1	candidates					1588:1597	the lead candidates	1579:1597	the lead candidates for validation	1579:1612	These N-glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.					
29992770	4	80	theme	12	579:580	arg1	age-					582:585	age-	582:585	age-	582:585	EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age- and sex-matched controls are collected.					
29976678	6	0	theme	hinge	858:862	arg1	region					864:869	the E domain I-II hinge region	840:869	the E domain I-II hinge region	840:869	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	14	1	from	insertions	2208:2217	arg1	regions					2232:2238	different regions	2222:2238	different regions of the envelope protein	2222:2262	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	5	2	theme	FLAG	639:642	arg1	insertions					644:653	FLAG insertions	639:653	FLAG insertions in prM	639:660	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	7	3	theme	sensitivity	1075:1085	arg1	pattern					1049:1055	the pattern	1045:1055	the pattern of neutralization sensitivity	1045:1085	We measured the antibody binding efficiency and found that this closely matched the pattern of neutralization sensitivity.					
29976678	3	4	theme	prM	479:481	arg1	regions					514:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	13	5	theme	envelope	2057:2064	arg1	surface					2074:2080	the envelope protein surface	2053:2080	the envelope protein surface	2053:2080	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	6	6	theme	domain	846:851	arg1	region					864:869	the E domain I-II hinge region	840:869	the E domain I-II hinge region	840:869	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	13	7	theme	surface	2074:2080	arg1	regions					2042:2048	all regions	2038:2048	all regions of the envelope protein surface or even buried epitopes	2038:2104	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	13	7	theme	surface	2074:2080	arg1	targets					2137:2143	neutralization targets	2122:2143	neutralization targets	2122:2143	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	12	8	theme	envelope	1937:1944	arg1	target					1982:1987	the dominant target	1969:1987	the dominant target of the humoral immune response	1969:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	12	8	theme	envelope	1937:1944	arg1	glycoprotein					1946:1957	the envelope glycoprotein	1933:1957	the envelope glycoprotein	1933:1957	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	4	9	theme	screen	622:627	arg1	results					565:571	the results	561:571	the results of our recent transposon insertional mutagenesis screen	561:627	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	13	10	theme	epitopes	2097:2104	arg1	regions					2042:2048	all regions	2038:2048	all regions of the envelope protein surface or even buried epitopes	2038:2104	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	13	10	theme	epitopes	2097:2104	arg1	targets					2137:2143	neutralization targets	2122:2143	neutralization targets	2122:2143	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	1	11	theme	humoral	170:176	arg1	response					185:192	the humoral immune response	166:192	the humoral immune response	166:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	8	12	theme	antibody	1138:1145	arg1	binding					1147:1153	antibody binding	1138:1153	antibody binding to a nearby epitope or its capacity to serve as a neutralization target	1138:1225	We determined that E glycosylation did not affect antibody binding to a nearby epitope or its capacity to serve as a neutralization target.					
29976678	2	13	gly	glycoproteins	244:256	arg1	glycoproteins					244:256	these glycoproteins	238:256	these glycoproteins	238:256	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	14	14	theme	envelope	2247:2254	arg1	protein					2256:2262	the envelope protein	2243:2262	the envelope protein	2243:2262	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	2	15	theme	sequences	298:306	arg1	insertion					270:278	insertion	270:278	insertion of linear epitope sequences	270:306	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	1	16	theme	response	185:192	arg1	glycoproteins					126:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	1	16	theme	response	185:192	arg1	target					156:161	the primary target	144:161	the primary target of the humoral immune response	144:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	4	17	theme	recent	580:585	arg1	screen					622:627	our recent transposon insertional mutagenesis screen	576:627	our recent transposon insertional mutagenesis screen	576:627	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	2	18	theme	linear	283:288	arg1	sequences					298:306	linear epitope sequences	283:306	linear epitope sequences	283:306	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	15	19	theme	protein	2474:2480	arg1	region					2451:2456	even a buried region	2437:2456	even a buried region of the envelope protein	2437:2480	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	8	20	theme	nearby	1160:1165	arg1	epitope					1167:1173	a nearby epitope	1158:1173	a nearby epitope	1158:1173	We determined that E glycosylation did not affect antibody binding to a nearby epitope or its capacity to serve as a neutralization target.					
29976678	6	21	theme	FLAG	895:898	arg1	antibody					900:907	FLAG antibody	895:907	FLAG antibody	895:907	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	4	22	theme	insertional	598:608	arg1	screen					622:627	our recent transposon insertional mutagenesis screen	576:627	our recent transposon insertional mutagenesis screen	576:627	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	11	23	theme	virus	1764:1768	arg1	infections					1777:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	1	24	gly	glycoproteins	126:138	arg1	glycoproteins					126:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	1	24	gly	glycoproteins	126:138	arg1	target					156:161	the primary target	144:161	the primary target of the humoral immune response	144:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	15	25	theme	major	2366:2370	arg1	strength					2316:2323	the strength	2312:2323	the strength of antibody binding to an epitope	2312:2357	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	25	theme	major	2366:2370	arg1	determinant					2372:2382	the major determinant	2362:2382	the major determinant of the neutralization potential of an antibody	2362:2429	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	12	26	theme	particle	1888:1895	arg1	structure					1859:1867	The structure	1855:1867	The structure of the mature ZIKV particle	1855:1895	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	12	27	theme	humoral	1996:2002	arg1	response					2011:2018	the humoral immune response	1992:2018	the humoral immune response	1992:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	6	28	from	epitopes	805:812	arg1	domains					819:825	E domains I and II	817:834	E domains I and II	817:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	28	from	epitopes	805:812	arg1	II					833:834	II	833:834	II	833:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	28	from	epitopes	805:812	arg1	I					827:827	I	827:827	I	827:827	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	11	29	theme	attachment.IMPORTANCE	1737:1757	arg1	infections					1777:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	7	30	theme	binding	990:996	arg1	efficiency					998:1007	the antibody binding efficiency	977:1007	the antibody binding efficiency	977:1007	We measured the antibody binding efficiency and found that this closely matched the pattern of neutralization sensitivity.					
29976678	12	31	theme	response	2011:2018	arg1	target					1982:1987	the dominant target	1969:1987	the dominant target of the humoral immune response	1969:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	12	31	theme	response	2011:2018	arg1	glycoprotein					1946:1957	the envelope glycoprotein	1933:1957	the envelope glycoprotein	1933:1957	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	9	32	with	viruses	1270:1276	arg1	insertions					1296:1305	FLAG epitope insertions	1283:1305	FLAG epitope insertions in a buried region of E protein domain III	1283:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	3	33	with	mutants	420:426	arg1	epitope					442:448	the FLAG epitope	433:448	the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions	433:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	12	34	theme	dominant	1973:1980	arg1	target					1982:1987	the dominant target	1969:1987	the dominant target of the humoral immune response	1969:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	12	34	theme	dominant	1973:1980	arg1	glycoprotein					1946:1957	the envelope glycoprotein	1933:1957	the envelope glycoprotein	1933:1957	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	11	35	theme	cell	1670:1673	arg1	entry					1675:1679	cell entry	1670:1679	cell entry	1670:1679	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	15	36	theme	binding	2337:2343	arg1	strength					2316:2323	the strength	2312:2323	the strength of antibody binding to an epitope	2312:2357	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	36	theme	binding	2337:2343	arg1	determinant					2372:2382	the major determinant	2362:2382	the major determinant of the neutralization potential of an antibody	2362:2429	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	1	37	theme	primary	148:154	arg1	glycoproteins					126:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	1	37	theme	primary	148:154	arg1	target					156:161	the primary target	144:161	the primary target of the humoral immune response	144:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	14	38	theme	epitope	2200:2206	arg1	insertions					2208:2217	epitope insertions	2200:2217	epitope insertions in different regions of the envelope protein	2200:2262	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	0	39	theme	Epitope	88:94	arg1	Insertions					96:105	Linear Epitope Insertions	81:105	Linear Epitope Insertions	81:105	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	15	40	theme	antibody	2422:2429	arg1	potential					2406:2414	the neutralization potential	2387:2414	the neutralization potential of an antibody	2387:2429	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	6	41	theme	neutralization	923:936	arg1	sensitivity					938:948	the neutralization sensitivity	919:948	the neutralization sensitivity	919:948	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	14	42	theme	mutants	2187:2193	arg1	panel					2173:2177	a panel	2171:2177	a panel of ZIKV mutants	2171:2193	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	15	43	theme	envelope	2539:2546	arg1	glycan					2548:2553	the sole potential envelope glycan	2520:2553	the sole potential envelope glycan	2520:2553	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	12	44	theme	immune	2004:2009	arg1	response					2011:2018	the humoral immune response	1992:2018	the humoral immune response	1992:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	3	45	theme	ZIKV	415:418	arg1	mutants					420:426	ZIKV mutants	415:426	ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions	415:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	15	46	theme	sole	2524:2527	arg1	glycan					2548:2553	the sole potential envelope glycan	2520:2553	the sole potential envelope glycan	2520:2553	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	9	47	theme	infectious	1259:1268	arg1	viruses					1270:1276	infectious viruses	1259:1276	infectious viruses with FLAG epitope insertions in a buried region of E protein domain III	1259:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	9	48	from	insertions	1296:1305	arg1	region					1319:1324	a buried region	1310:1324	a buried region of E protein domain III	1310:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	15	49	theme	neutralization	2391:2404	arg1	potential					2406:2414	the neutralization potential	2387:2414	the neutralization potential of an antibody	2387:2429	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	1	50	theme	Zika	108:111	arg1	ZIKV					120:123	ZIKV	120:123	ZIKV	120:123	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	1	50	theme	Zika	108:111	arg1	virus					113:117	Zika virus	108:117	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	3	51	theme	FLAG	437:440	arg1	epitope					442:448	the FLAG epitope	433:448	the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions	433:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	0	52	theme	Virus	13:17	arg1	Determinants					34:45	Zika Virus Neutralization Determinants	8:45	Zika Virus Neutralization Determinants	8:45	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	9	53	theme	epitope	1288:1294	arg1	insertions					1296:1305	FLAG epitope insertions	1283:1305	FLAG epitope insertions in a buried region of E protein domain III	1283:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	5	54	theme	protein	752:758	arg1	sites					760:764	numerous surface-exposed E protein sites	725:764	numerous surface-exposed E protein sites	725:764	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	2	55	theme	antibodies	329:338	arg1	potential					316:324	the potential	312:324	the potential of antibodies that bind these epitopes to inhibit infection	312:384	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	2	55	theme	antibodies	329:338	arg1	capacity					226:233	the capacity	222:233	the capacity of these glycoproteins to tolerate insertion of linear epitope sequences	222:306	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	0	56	theme	Glycoprotein	52:63	arg1	Mutants					65:71	Glycoprotein Mutants	52:71	Glycoprotein Mutants Bearing Linear Epitope Insertions	52:105	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	9	57	theme	buried	1312:1317	arg1	region					1319:1324	a buried region	1310:1324	a buried region of E protein domain III	1310:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	11	58	theme	ZIKV	1771:1774	arg1	infections					1777:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	15	59	theme	envelope	2465:2472	arg1	protein					2474:2480	the envelope protein	2461:2480	the envelope protein	2461:2480	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	6	60	theme	FLAG	800:803	arg1	epitopes					805:812	FLAG epitopes	800:812	FLAG epitopes in E domains I and II	800:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	11	61	theme	neutralization	1639:1652	arg1	activity					1654:1661	antibody neutralization activity	1630:1661	antibody neutralization activity	1630:1661	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	8	62	gly	glycosylation	1109:1121	arg1	antibody					1138:1145	antibody binding	1138:1153	antibody binding to a nearby epitope or its capacity to serve as a neutralization target	1138:1225	We determined that E glycosylation did not affect antibody binding to a nearby epitope or its capacity to serve as a neutralization target.					
29976678	11	63	theme	severe	1808:1813	arg1	defects					1821:1827	severe birth defects	1808:1827	severe birth defects	1808:1827	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	9	64	theme	protein	1331:1337	arg1	domain					1339:1344	E protein domain III	1329:1348	E protein domain III	1329:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	6	65	from	region	864:869	arg1	domains					819:825	E domains I and II	817:834	E domains I and II	817:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	65	from	region	864:869	arg1	II					833:834	II	833:834	II	833:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	65	from	region	864:869	arg1	I					827:827	I	827:827	I	827:827	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	7	66	theme	neutralization	1060:1073	arg1	sensitivity					1075:1085	neutralization sensitivity	1060:1085	neutralization sensitivity	1060:1085	We measured the antibody binding efficiency and found that this closely matched the pattern of neutralization sensitivity.					
29976678	15	67	theme	buried	2444:2449	arg1	region					2451:2456	even a buried region	2437:2456	even a buried region of the envelope protein	2437:2480	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	68	theme	antibody	2586:2593	arg1	binding					2595:2601	nearby epitope antibody binding	2571:2601	nearby epitope antibody binding	2571:2601	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	4	69	theme	insertion	527:535	arg1	locations					537:545	The insertion locations	523:545	The insertion locations	523:545	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	10	70	theme	V5	1501:1502	arg1	antibodies					1504:1513	V5 antibodies	1501:1513	V5 antibodies	1501:1513	Furthermore, this virus was efficiently neutralized by V5 antibodies, highlighting that even buried epitopes can function as neutralization targets.					
29976678	3	71	theme	E	498:498	arg1	regions					514:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	13	72	theme	protein	2066:2072	arg1	surface					2074:2080	the envelope protein surface	2053:2080	the envelope protein surface	2053:2080	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	8	73	theme	E	1107:1107	arg1	glycosylation					1109:1121	E glycosylation	1107:1121	E glycosylation	1107:1121	We determined that E glycosylation did not affect antibody binding to a nearby epitope or its capacity to serve as a neutralization target.					
29976678	6	74	theme	E	844:844	arg1	region					864:869	the E domain I-II hinge region	840:869	the E domain I-II hinge region	840:869	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	3	75	theme	envelope	488:495	arg1	E					498:498	envelope (E)	488:499	envelope (E)	488:499	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	1	76	theme	immune	178:183	arg1	response					185:192	the humoral immune response	166:192	the humoral immune response	166:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	15	77	theme	epitope	2578:2584	arg1	binding					2595:2601	nearby epitope antibody binding	2571:2601	nearby epitope antibody binding	2571:2601	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	4	78	theme	mutagenesis	610:620	arg1	screen					622:627	our recent transposon insertional mutagenesis screen	576:627	our recent transposon insertional mutagenesis screen	576:627	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	5	79	theme	surface-exposed	734:748	arg1	sites					760:764	numerous surface-exposed E protein sites	725:764	numerous surface-exposed E protein sites	725:764	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	14	80	theme	protein	2256:2262	arg1	regions					2232:2238	different regions	2222:2238	different regions of the envelope protein	2222:2262	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	9	81	theme	V5	1369:1370	arg1	epitope					1372:1378	the V5 epitope	1365:1378	the V5 epitope	1365:1378	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	11	82	theme	cell	1732:1735	arg1	infections					1777:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	3	83	theme	glycoprotein	501:512	arg1	regions					514:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	12	84	theme	virion	1907:1912	arg1	surface					1914:1920	a virion surface	1905:1920	a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response	1905:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	5	85	theme	viral	679:683	arg1	fitness					685:691	viral fitness	679:691	viral fitness	679:691	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	4	86	theme	transposon	587:596	arg1	screen					622:627	our recent transposon insertional mutagenesis screen	576:627	our recent transposon insertional mutagenesis screen	576:627	The insertion locations were based on the results of our recent transposon insertional mutagenesis screen.					
29976678	10	87	theme	buried	1539:1544	arg1	targets					1586:1592	neutralization targets	1571:1592	neutralization targets	1571:1592	Furthermore, this virus was efficiently neutralized by V5 antibodies, highlighting that even buried epitopes can function as neutralization targets.					
29976678	10	87	theme	buried	1539:1544	arg1	epitopes					1546:1553	even buried epitopes	1534:1553	even buried epitopes	1534:1553	Furthermore, this virus was efficiently neutralized by V5 antibodies, highlighting that even buried epitopes can function as neutralization targets.					
29976678	7	88	theme	antibody	981:988	arg1	efficiency					998:1007	the antibody binding efficiency	977:1007	the antibody binding efficiency	977:1007	We measured the antibody binding efficiency and found that this closely matched the pattern of neutralization sensitivity.					
29976678	12	89	theme	ZIKV	1883:1886	arg1	particle					1888:1895	the mature ZIKV particle	1872:1895	the mature ZIKV particle	1872:1895	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	11	90	theme	Zika	1759:1762	arg1	infections					1777:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	cell attachment.IMPORTANCE Zika virus (ZIKV) infections	1732:1786	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	2	91	theme	glycoproteins	244:256	arg1	potential					316:324	the potential	312:324	the potential of antibodies that bind these epitopes to inhibit infection	312:384	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	2	91	theme	glycoproteins	244:256	arg1	capacity					226:233	the capacity	222:233	the capacity of these glycoproteins to tolerate insertion of linear epitope sequences	222:306	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	2	92	theme	epitope	290:296	arg1	sequences					298:306	linear epitope sequences	283:306	linear epitope sequences	283:306	In this study, we explored the capacity of these glycoproteins to tolerate insertion of linear epitope sequences and the potential of antibodies that bind these epitopes to inhibit infection.					
29976678	16	93	theme	important	2655:2663	arg1	insights					2665:2672	important insights	2655:2672	important insights into our understanding of how antibodies neutralize ZIKV	2655:2729	Furthermore, this work provides important insights into our understanding of how antibodies neutralize ZIKV.					
29976678	3	94	gly	glycoprotein	501:512	arg1	glycoprotein					501:512	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	the premembrane (prM) and envelope (E) glycoprotein regions	462:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	14	95	theme	different	2222:2230	arg1	regions					2232:2238	different regions	2222:2238	different regions of the envelope protein	2222:2262	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	0	96	theme	Linear	81:86	arg1	Insertions					96:105	Linear Epitope Insertions	81:105	Linear Epitope Insertions	81:105	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	5	97	theme	numerous	725:732	arg1	sites					760:764	numerous surface-exposed E protein sites	725:764	numerous surface-exposed E protein sites	725:764	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	15	98	theme	antibody	2328:2335	arg1	binding					2337:2343	antibody binding	2328:2343	antibody binding	2328:2343	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	99	theme	potential	2529:2537	arg1	glycan					2548:2553	the sole potential envelope glycan	2520:2553	the sole potential envelope glycan	2520:2553	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	14	100	theme	ZIKV	2182:2185	arg1	mutants					2187:2193	ZIKV mutants	2182:2193	ZIKV mutants	2182:2193	To test this, we created a panel of ZIKV mutants with epitope insertions in different regions of the envelope protein.					
29976678	12	101	theme	mature	1876:1881	arg1	particle					1888:1895	the mature ZIKV particle	1872:1895	the mature ZIKV particle	1872:1895	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	10	102	theme	neutralization	1571:1584	arg1	targets					1586:1592	neutralization targets	1571:1592	neutralization targets	1571:1592	Furthermore, this virus was efficiently neutralized by V5 antibodies, highlighting that even buried epitopes can function as neutralization targets.					
29976678	10	102	theme	neutralization	1571:1584	arg1	epitopes					1546:1553	even buried epitopes	1534:1553	even buried epitopes	1534:1553	Furthermore, this virus was efficiently neutralized by V5 antibodies, highlighting that even buried epitopes can function as neutralization targets.					
29976678	0	103	theme	Zika	8:11	arg1	Determinants					34:45	Zika Virus Neutralization Determinants	8:45	Zika Virus Neutralization Determinants	8:45	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	15	104	theme	potential	2406:2414	arg1	strength					2316:2323	the strength	2312:2323	the strength of antibody binding to an epitope	2312:2357	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	104	theme	potential	2406:2414	arg1	impact					2564:2569	impact	2564:2569	impact	2564:2569	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	15	104	theme	potential	2406:2414	arg1	determinant					2372:2382	the major determinant	2362:2382	the major determinant of the neutralization potential of an antibody	2362:2429	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	0	105	theme	Neutralization	19:32	arg1	Determinants					34:45	Zika Virus Neutralization Determinants	8:45	Zika Virus Neutralization Determinants	8:45	Probing Zika Virus Neutralization Determinants with Glycoprotein Mutants Bearing Linear Epitope Insertions.					
29976678	9	106	theme	FLAG	1283:1286	arg1	insertions					1296:1305	FLAG epitope insertions	1283:1305	FLAG epitope insertions in a buried region of E protein domain III	1283:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	1	107	theme	virus	113:117	arg1	glycoproteins					126:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins	108:138	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	1	107	theme	virus	113:117	arg1	target					156:161	the primary target	144:161	the primary target of the humoral immune response	144:192	Zika virus (ZIKV) glycoproteins are the primary target of the humoral immune response.					
29976678	5	108	theme	E	750:750	arg1	sites					760:764	numerous surface-exposed E protein sites	725:764	numerous surface-exposed E protein sites	725:764	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	11	109	theme	neurological	1833:1844	arg1	disease					1846:1852	neurological disease	1833:1852	neurological disease	1833:1852	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	6	110	dep	domains	819:825	arg1	domains					819:825	E domains I and II	817:834	E domains I and II	817:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	110	dep	domains	819:825	arg1	II					833:834	II	833:834	II	833:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	110	dep	domains	819:825	arg1	I					827:827	I	827:827	I	827:827	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	13	111	theme	buried	2090:2095	arg1	epitopes					2097:2104	even buried epitopes	2085:2104	even buried epitopes	2085:2104	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	12	112	gly	glycoprotein	1946:1957	arg1	target					1982:1987	the dominant target	1969:1987	the dominant target of the humoral immune response	1969:2018	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	12	112	gly	glycoprotein	1946:1957	arg1	glycoprotein					1946:1957	the envelope glycoprotein	1933:1957	the envelope glycoprotein	1933:1957	The structure of the mature ZIKV particle reveals a virion surface covered by the envelope glycoprotein, which is the dominant target of the humoral immune response.					
29976678	15	113	theme	nearby	2571:2576	arg1	binding					2595:2601	nearby epitope antibody binding	2571:2601	nearby epitope antibody binding	2571:2601	In characterizing these viruses, we found that the strength of antibody binding to an epitope is the major determinant of the neutralization potential of an antibody, that even a buried region of the envelope protein can be efficiently targeted, and that the sole potential envelope glycan does not impact nearby epitope antibody binding and neutralization.					
29976678	6	114	theme	E	817:817	arg1	domains					819:825	E domains I and II	817:834	E domains I and II	817:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	114	theme	E	817:817	arg1	II					833:834	II	833:834	II	833:834	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	6	114	theme	E	817:817	arg1	I					827:827	I	827:827	I	827:827	We observed that mutants bearing FLAG epitopes in E domains I and II and the E domain I-II hinge region were all neutralized by FLAG antibody; however, the neutralization sensitivity varied highly.					
29976678	11	115	theme	activity	1654:1661	arg1	timing					1620:1625	the timing	1616:1625	the timing of antibody neutralization activity during cell entry	1616:1679	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	3	116	theme	premembrane	466:476	arg1	prM					479:481	premembrane (prM)	466:482	premembrane (prM)	466:482	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	13	117	theme	neutralization	2122:2135	arg1	regions					2042:2048	all regions	2038:2048	all regions of the envelope protein surface or even buried epitopes	2038:2104	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	13	117	theme	neutralization	2122:2135	arg1	targets					2137:2143	neutralization targets	2122:2143	neutralization targets	2122:2143	It is unclear if all regions of the envelope protein surface or even buried epitopes can function as neutralization targets.					
29976678	11	118	theme	antibody	1630:1637	arg1	activity					1654:1661	antibody neutralization activity	1630:1661	antibody neutralization activity	1630:1661	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	9	119	theme	E	1329:1329	arg1	domain					1339:1344	E protein domain III	1329:1348	E protein domain III	1329:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29976678	8	120	theme	neutralization	1205:1218	arg1	target					1220:1225	a neutralization target	1203:1225	a neutralization target	1203:1225	We determined that E glycosylation did not affect antibody binding to a nearby epitope or its capacity to serve as a neutralization target.					
29976678	3	121	theme	mutants	420:426	arg1	panel					406:410	a panel	404:410	a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions	404:520	We first created a panel of ZIKV mutants with the FLAG epitope inserted in the premembrane (prM) and envelope (E) glycoprotein regions.					
29976678	5	122	from	insertions	644:653	arg1	prM					658:660	prM	658:660	prM	658:660	Although FLAG insertions in prM greatly impaired viral fitness, this sequence was tolerated in numerous surface-exposed E protein sites.					
29976678	11	123	theme	birth	1815:1819	arg1	defects					1821:1827	severe birth defects	1808:1827	severe birth defects	1808:1827	Finally, we analyzed the timing of antibody neutralization activity during cell entry and found that all antibodies blocked a step after cell attachment.IMPORTANCE Zika virus (ZIKV) infections are associated with severe birth defects and neurological disease.					
29976678	9	124	theme	domain	1339:1344	arg1	region					1319:1324	a buried region	1310:1324	a buried region of E protein domain III	1310:1348	Although we could not generate infectious viruses with FLAG epitope insertions in a buried region of E protein domain III, we found that the V5 epitope could be inserted at this site without greatly impacting fitness.					
29769533	1	0	theme	enzymatic	330:338	arg1	processing					340:349	enzymatic processing	330:349	enzymatic processing of the N-glycan shield	330:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	4	1	theme	native-like	846:856	arg1	processing					865:874	native-like glycan processing	846:874	native-like glycan processing	846:874	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	2	2	theme	akin	533:536	arg1	conformation					520:531	prefusion conformation	510:531	prefusion conformation akin to furin-cleaved Env trimers	510:565	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	1	3	theme	glycoprotein	153:164	arg1	step					182:185	an essential step	169:185	an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield	169:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	3	theme	glycoprotein	153:164	arg1	cleavage					124:131	Furin cleavage	118:131	Furin cleavage of the HIV envelope glycoprotein	118:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	4	theme	native-like	241:251	arg1	trimers					266:272	well-folded, native-like glycosylated trimers	228:272	trimers	266:272	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	3	5	with	NFL.664	599:605	arg1	bNAbs					644:648	two potent bNAbs	633:648	two potent bNAbs	633:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	2	6	dep	trimer	442:447	arg1	mimic					449:453	mimic	449:453	mimic	449:453	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	4	7	from	maturation	916:925	arg1	pathway					944:950	the secretory pathway	930:950	the secretory pathway	930:950	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	5	8	theme	immunogen	1134:1142	arg1	candidates					1144:1153	vaccine immunogen candidates	1126:1153	vaccine immunogen candidates	1126:1153	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	1	9	theme	glycosylated	253:264	arg1	trimers					266:272	well-folded, native-like glycosylated trimers	228:272	trimers	266:272	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	0	10	theme	cleaved	102:108	arg1	trimer					110:115	the native cleaved trimer	91:115	the native cleaved trimer	91:115	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	5	11	theme	quaternary	1004:1013	arg1	protein					1015:1021	quaternary protein and carbohydrate structures	1004:1049	protein	1015:1021	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	0	12	gly	glycoprotein	62:73	arg1	glycoprotein					62:73	the glycoprotein architecture	58:86	the glycoprotein architecture of the native cleaved trimer	58:115	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	4	13	dep	glycan	819:824	arg1	shield					826:831	shield	826:831	shield	826:831	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	1	14	theme	trimers	266:272	arg1	formation					215:223	formation	215:223	formation of well-folded, native-like glycosylated trimers	215:272	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	0	15	theme	native	95:100	arg1	trimer					110:115	the native cleaved trimer	91:115	the native cleaved trimer	91:115	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	3	16	from	resolution	617:626	arg1	structure					580:588	The crystal structure	568:588	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs	568:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	3	16	from	resolution	617:626	arg1	NFL.664					599:605	BG505 NFL.664	593:605	BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs	593:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	4	17	theme	glycan	858:863	arg1	processing					865:874	native-like glycan processing	846:874	native-like glycan processing	846:874	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	5	18	theme	NFL	980:982	arg1	trimers					988:994	cleavage-independent NFL Env trimers	959:994	cleavage-independent NFL Env trimers	959:994	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	3	19	from	structure	580:588	arg1	resolution					617:626	3.39 Å resolution	610:626	3.39 Å resolution	610:626	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	2	20	theme	closed-form	483:493	arg1	"					494:494	a "closed-form"	480:494	a "closed-form"	480:494	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	1	21	theme	N-glycan	358:365	arg1	processing					340:349	enzymatic processing	330:349	enzymatic processing of the N-glycan shield	330:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	4	22	theme	glycan	819:824	arg1	analysis					803:810	Quantitative site-specific analysis	776:810	Quantitative site-specific analysis of the glycan shield	776:831	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	5	23	theme	Env	984:986	arg1	trimers					988:994	cleavage-independent NFL Env trimers	959:994	cleavage-independent NFL Env trimers	959:994	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	0	24	theme	trimer	110:115	arg1	architecture					75:86	the glycoprotein architecture	58:86	the glycoprotein architecture of the native cleaved trimer	58:115	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	5	25	theme	vaccine	1126:1132	arg1	candidates					1144:1153	vaccine immunogen candidates	1126:1153	vaccine immunogen candidates	1126:1153	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	1	26	gly	glycosylated	253:264	arg1	trimers					266:272	well-folded, native-like glycosylated trimers	228:272	trimers	266:272	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	27	theme	essential	172:180	arg1	step					182:185	an essential step	169:185	an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield	169:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	27	theme	essential	172:180	arg1	cleavage					124:131	Furin cleavage	118:131	Furin cleavage of the HIV envelope glycoprotein	118:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	2	28	theme	Env	438:440	arg1	NFL.664					462:468	BG505 NFL.664	456:468	BG505 NFL.664	456:468	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	2	28	theme	Env	438:440	arg1	trimer					442:447	a cleavage-independent, stabilized, soluble Env trimer mimic	394:453	trimer	442:447	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	3	29	theme	PGV19	687:691	arg1	epitopes					675:682	the full epitopes	666:682	the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively	666:773	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	3	30	theme	PGT122	697:702	arg1	epitopes					675:682	the full epitopes	666:682	the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively	666:773	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	2	31	theme	soluble	430:436	arg1	NFL.664					462:468	BG505 NFL.664	456:468	BG505 NFL.664	456:468	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	2	31	theme	soluble	430:436	arg1	trimer					442:447	a cleavage-independent, stabilized, soluble Env trimer mimic	394:453	trimer	442:447	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	1	32	from	constraints	284:294	arg1	peptide					310:316	the fusion peptide	299:316	the fusion peptide	299:316	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	0	33	theme	cleavage-independent	15:34	arg1	Env					40:42	a cleavage-independent HIV Env	13:42	a cleavage-independent HIV Env	13:42	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	3	34	theme	receptor	720:727	arg1	site					737:740	the receptor binding site	716:740	the receptor binding site	716:740	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	5	35	theme	carbohydrate	1027:1038	arg1	structures					1040:1049	quaternary protein and carbohydrate structures	1004:1049	structures	1040:1049	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	1	36	theme	cell	191:194	arg1	entry					196:200	cell entry	191:200	cell entry	191:200	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	2	37	theme	prefusion	510:518	arg1	conformation					520:531	prefusion conformation	510:531	prefusion conformation akin to furin-cleaved Env trimers	510:565	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	2	38	theme	cleavage-independent	396:415	arg1	NFL.664					462:468	BG505 NFL.664	456:468	BG505 NFL.664	456:468	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	2	38	theme	cleavage-independent	396:415	arg1	trimer					442:447	a cleavage-independent, stabilized, soluble Env trimer mimic	394:453	trimer	442:447	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	0	39	theme	Env	40:42	arg1	Structure					0:8	Structure	0:8	Structure of a cleavage-independent HIV Env	0:42	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	5	40	theme	cleavage-independent	959:978	arg1	trimers					988:994	cleavage-independent NFL Env trimers	959:994	cleavage-independent NFL Env trimers	959:994	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	4	41	theme	secretory	934:942	arg1	pathway					944:950	the secretory pathway	930:950	the secretory pathway	930:950	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	4	42	theme	site-specific	789:801	arg1	analysis					803:810	Quantitative site-specific analysis	776:810	Quantitative site-specific analysis of the glycan shield	776:831	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	0	43	theme	HIV	36:38	arg1	Env					40:42	a cleavage-independent HIV Env	13:42	a cleavage-independent HIV Env	13:42	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	1	44	theme	fusion	303:308	arg1	peptide					310:316	the fusion peptide	299:316	the fusion peptide	299:316	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	3	45	theme	crystal	572:578	arg1	structure					580:588	The crystal structure	568:588	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs	568:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	3	46	theme	N332	746:749	arg1	supersite					751:759	N332 supersite	746:759	N332 supersite	746:759	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	2	47	theme	Env	555:557	arg1	trimers					559:565	furin-cleaved Env trimers	541:565	furin-cleaved Env trimers	541:565	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	3	48	theme	3.39 Å	610:615	arg1	resolution					617:626	3.39 Å resolution	610:626	3.39 Å resolution	610:626	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	5	49	theme	similar	1051:1057	arg1	protein					1015:1021	quaternary protein and carbohydrate structures	1004:1049	protein	1015:1021	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	1	50	theme	Furin	118:122	arg1	step					182:185	an essential step	169:185	an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield	169:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	50	theme	Furin	118:122	arg1	cleavage					124:131	Furin cleavage	118:131	Furin cleavage of the HIV envelope glycoprotein	118:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	3	51	theme	binding	729:735	arg1	site					737:740	the receptor binding site	716:740	the receptor binding site	716:740	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	2	52	theme	furin-cleaved	541:553	arg1	trimers					559:565	furin-cleaved Env trimers	541:565	furin-cleaved Env trimers	541:565	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	2	53	dep	cleavage-independent	396:415	arg1	stabilized					418:427	stabilized	418:427	stabilized	418:427	Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers.					
29769533	1	54	dep	N-glycan	358:365	arg1	shield					367:372	shield	367:372	shield	367:372	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	4	55	theme	furin-independent	898:914	arg1	maturation					916:925	furin-independent maturation	898:925	furin-independent maturation in the secretory pathway	898:950	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	5	56	theme	native	1066:1071	arg1	spike					1079:1083	the native viral spike	1062:1083	the native viral spike that further validate their potential as vaccine immunogen candidates	1062:1153	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	4	57	theme	Quantitative	776:787	arg1	analysis					803:810	Quantitative site-specific analysis	776:810	Quantitative site-specific analysis of the glycan shield	776:831	Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway.					
29769533	1	58	gly	glycoprotein	153:164	arg1	glycoprotein					153:164	the HIV envelope glycoprotein	136:164	the HIV envelope glycoprotein	136:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	5	59	theme	viral	1073:1077	arg1	spike					1079:1083	the native viral spike	1062:1083	the native viral spike that further validate their potential as vaccine immunogen candidates	1062:1153	Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates.					
29769533	3	60	theme	NFL.664	599:605	arg1	structure					580:588	The crystal structure	568:588	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs	568:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	3	61	theme	potent	637:642	arg1	bNAbs					644:648	two potent bNAbs	633:648	two potent bNAbs	633:648	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	1	62	theme	HIV	140:142	arg1	glycoprotein					153:164	the HIV envelope glycoprotein	136:164	the HIV envelope glycoprotein	136:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	1	63	theme	well-folded	228:238	arg1	trimers					266:272	well-folded, native-like glycosylated trimers	228:272	trimers	266:272	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29769533	0	64	theme	glycoprotein	62:73	arg1	architecture					75:86	the glycoprotein architecture	58:86	the glycoprotein architecture of the native cleaved trimer	58:115	Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.					
29769533	3	65	theme	full	670:673	arg1	epitopes					675:682	the full epitopes	666:682	the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively	666:773	The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively.					
29769533	1	66	theme	envelope	144:151	arg1	glycoprotein					153:164	the HIV envelope glycoprotein	136:164	the HIV envelope glycoprotein	136:164	Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield.					
29757379	6	0	theme	QSOX1	909:913	arg1	secretion					915:923	QSOX1 secretion	909:923	QSOX1 secretion from fibroblasts and other cell types	909:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	9	1	from	example	1357:1363	arg1	exit					1405:1408	Golgi exit	1399:1408	Golgi exit	1399:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	1	2	from	formation	156:164	arg1	substrates					196:205	protein substrates	188:205	protein substrates	188:205	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	9	3	theme	control	1484:1490	arg1	understanding					1432:1444	an understanding	1429:1444	an understanding of late secretory sorting and quality control	1429:1490	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	8	4	theme	transmembrane	1171:1183	arg1	dispensable					1196:1206	dispensable	1196:1206	dispensable	1196:1206	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	8	4	theme	transmembrane	1171:1183	arg1	segment					1185:1191	The QSOX1 transmembrane segment	1161:1191	The QSOX1 transmembrane segment	1161:1191	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	3	5	theme	extracellular	488:500	arg1	assembly					509:516	extracellular matrix assembly	488:516	extracellular matrix assembly	488:516	QSOX1 is upregulated in quiescent fibroblast cells and secreted into the extracellular environment, where it contributes to extracellular matrix assembly.					
29757379	9	6	theme	Golgi	1399:1403	arg1	exit					1405:1408	Golgi exit	1399:1408	Golgi exit	1399:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	0	7	theme	Golgi	84:88	arg1	localization					90:101	Golgi localization	84:101	secretion but not Golgi localization	66:101	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	8	8	dep	luminal	1255:1261	arg1	variants					1281:1288	variants	1281:1288	variants	1281:1288	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	9	9	theme	key	1353:1355	arg1	example					1357:1363	a key example	1351:1363	a key example of the effect of glycosylation on Golgi exit	1351:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	9	10	theme	secretory	1454:1462	arg1	sorting					1464:1470	late secretory sorting	1449:1470	late secretory sorting	1449:1470	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	7	11	theme	reticulum	1069:1077	arg1	control					1087:1093	endoplasmic reticulum quality control	1057:1093	endoplasmic reticulum quality control	1057:1093	Notably, QSOX1 lacking a glycan at this site arrives at the Golgi, suggesting that it passes endoplasmic reticulum quality control but is not further transported to the cell surface for secretion.					
29757379	9	12	theme	late	1449:1452	arg1	sorting					1464:1470	late secretory sorting	1449:1470	late secretory sorting	1449:1470	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	8	13	theme	QSOX1	1165:1169	arg1	dispensable					1196:1206	dispensable	1196:1206	dispensable	1196:1206	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	8	13	theme	QSOX1	1165:1169	arg1	segment					1185:1191	The QSOX1 transmembrane segment	1161:1191	The QSOX1 transmembrane segment	1161:1191	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	7	14	theme	quality	1079:1085	arg1	control					1087:1093	endoplasmic reticulum quality control	1057:1093	endoplasmic reticulum quality control	1057:1093	Notably, QSOX1 lacking a glycan at this site arrives at the Golgi, suggesting that it passes endoplasmic reticulum quality control but is not further transported to the cell surface for secretion.					
29757379	5	15	theme	QSOX1	802:806	arg1	trafficking					808:818	QSOX1 trafficking	802:818	QSOX1 trafficking	802:818	To achieve a better understanding of factors that dictate QSOX1 localization and function, we aimed to determine how post-translational modifications affect QSOX1 trafficking and activity.					
29757379	8	16	theme	Golgi	1212:1216	arg1	localization					1218:1229	Golgi localization	1212:1229	Golgi localization	1212:1229	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	0	17	theme	sulfhydryl	9:18	arg1	oxidase					20:26	Quiescin sulfhydryl oxidase 1	0:28	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	0	17	theme	sulfhydryl	9:18	arg1	QSOX1					31:35	QSOX1	31:35	QSOX1	31:35	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	6	18	theme	glycosylation	871:883	arg1	site					885:888	a highly conserved N-linked glycosylation site	843:888	a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types	843:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	3	19	theme	fibroblast	398:407	arg1	cells					409:413	quiescent fibroblast cells	388:413	quiescent fibroblast cells	388:413	QSOX1 is upregulated in quiescent fibroblast cells and secreted into the extracellular environment, where it contributes to extracellular matrix assembly.					
29757379	0	20	theme	Quiescin	0:7	arg1	oxidase					20:26	Quiescin sulfhydryl oxidase 1	0:28	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	0	20	theme	Quiescin	0:7	arg1	QSOX1					31:35	QSOX1	31:35	QSOX1	31:35	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	7	21	theme	endoplasmic	1057:1067	arg1	reticulum					1069:1077	endoplasmic reticulum	1057:1077	endoplasmic reticulum quality control	1057:1093	Notably, QSOX1 lacking a glycan at this site arrives at the Golgi, suggesting that it passes endoplasmic reticulum quality control but is not further transported to the cell surface for secretion.					
29757379	2	22	theme	endoplasmic	286:296	arg1	reticulum					298:306	the endoplasmic reticulum	282:306	the endoplasmic reticulum	282:306	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	5	23	theme	QSOX1	703:707	arg1	localization					709:720	QSOX1 localization	703:720	QSOX1 localization	703:720	To achieve a better understanding of factors that dictate QSOX1 localization and function, we aimed to determine how post-translational modifications affect QSOX1 trafficking and activity.					
29757379	1	24	theme	disulfide	169:177	arg1	bonds					179:183	disulfide bonds	169:183	disulfide bonds	169:183	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	7	25	theme	cell	1133:1136	arg1	surface					1138:1144	the cell surface	1129:1144	the cell surface	1129:1144	Notably, QSOX1 lacking a glycan at this site arrives at the Golgi, suggesting that it passes endoplasmic reticulum quality control but is not further transported to the cell surface for secretion.					
29757379	6	26	from	types	957:961	arg1	secretion					915:923	QSOX1 secretion	909:923	QSOX1 secretion from fibroblasts and other cell types	909:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	3	27	theme	extracellular	437:449	arg1	environment					451:461	the extracellular environment	433:461	the extracellular environment	433:461	QSOX1 is upregulated in quiescent fibroblast cells and secreted into the extracellular environment, where it contributes to extracellular matrix assembly.					
29757379	0	28	theme	oxidase	20:26	arg1	mutation					48:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	2	29	with	enzymes	221:227	arg1	activities					242:251	related activities	234:251	related activities	234:251	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	5	30	theme	factors	682:688	arg1	understanding					665:677	a better understanding	656:677	a better understanding of factors that dictate QSOX1 localization and function	656:733	To achieve a better understanding of factors that dictate QSOX1 localization and function, we aimed to determine how post-translational modifications affect QSOX1 trafficking and activity.					
29757379	6	31	theme	N-linked	862:869	arg1	site					885:888	a highly conserved N-linked glycosylation site	843:888	a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types	843:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	8	32	theme	trafficking	1309:1319	arg1	behavior					1321:1328	the same trafficking behavior	1300:1328	the same trafficking behavior	1300:1328	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	8	33	theme	same	1304:1307	arg1	behavior					1321:1328	the same trafficking behavior	1300:1328	the same trafficking behavior	1300:1328	The QSOX1 transmembrane segment is dispensable for Golgi localization and secretion, as fully luminal and transmembrane variants displayed the same trafficking behavior.					
29757379	6	34	theme	cell	952:955	arg1	types					957:961	other cell types	946:961	other cell types	946:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	9	35	theme	quality	1476:1482	arg1	control					1484:1490	quality control	1476:1490	quality control	1476:1490	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	6	36	theme	conserved	852:860	arg1	site					885:888	a highly conserved N-linked glycosylation site	843:888	a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types	843:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	1	37	theme	protein	188:194	arg1	substrates					196:205	protein substrates	188:205	protein substrates	188:205	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	3	38	theme	quiescent	388:396	arg1	cells					409:413	quiescent fibroblast cells	388:413	quiescent fibroblast cells	388:413	QSOX1 is upregulated in quiescent fibroblast cells and secreted into the extracellular environment, where it contributes to extracellular matrix assembly.					
29757379	6	39	theme	other	946:950	arg1	types					957:961	other cell types	946:961	other cell types	946:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	2	40	theme	other	215:219	arg1	enzymes					221:227	other enzymes	215:227	other enzymes with related activities, which are commonly found in the endoplasmic reticulum	215:306	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	9	41	theme	sorting	1464:1470	arg1	understanding					1432:1444	an understanding	1429:1444	an understanding of late secretory sorting and quality control	1429:1490	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	0	42	theme	glycosite	38:46	arg1	mutation					48:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	2	43	located	found	273:277	arg2	activities					242:251	related activities	234:251	related activities	234:251	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	2	43	located	found	273:277	arg1	reticulum					298:306	the endoplasmic reticulum	282:306	the endoplasmic reticulum	282:306	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	6	44	from	fibroblasts	930:940	arg1	secretion					915:923	QSOX1 secretion	909:923	QSOX1 secretion from fibroblasts and other cell types	909:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	9	45	gly	glycosylation	1382:1394	arg1	Golgi					1399:1403	Golgi exit	1399:1408	Golgi exit	1399:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	1	46	theme	Quiescin	104:111	arg1	oxidase					124:130	Quiescin sulfhydryl oxidase 1	104:132	Quiescin sulfhydryl oxidase 1 (QSOX1)	104:140	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	1	46	theme	Quiescin	104:111	arg1	QSOX1					135:139	QSOX1	135:139	QSOX1	135:139	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	9	47	from	exit	1405:1408	arg1	example					1357:1363	a key example	1351:1363	a key example of the effect of glycosylation on Golgi exit	1351:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	1	48	theme	sulfhydryl	113:122	arg1	oxidase					124:130	Quiescin sulfhydryl oxidase 1	104:132	Quiescin sulfhydryl oxidase 1 (QSOX1)	104:140	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	1	48	theme	sulfhydryl	113:122	arg1	QSOX1					135:139	QSOX1	135:139	QSOX1	135:139	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	5	49	theme	better	658:663	arg1	understanding					665:677	a better understanding	656:677	a better understanding of factors that dictate QSOX1 localization and function	656:733	To achieve a better understanding of factors that dictate QSOX1 localization and function, we aimed to determine how post-translational modifications affect QSOX1 trafficking and activity.					
29757379	6	50	link	N-linked	862:869	arg1	site					885:888	a highly conserved N-linked glycosylation site	843:888	a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types	843:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	9	51	from	effect	1372:1377	arg1	exit					1405:1408	Golgi exit	1399:1408	Golgi exit	1399:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	6	52	gly	glycosylation	871:883	arg2	site					885:888	a highly conserved N-linked glycosylation site	843:888	a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types	843:961	We found a highly conserved N-linked glycosylation site to be required for QSOX1 secretion from fibroblasts and other cell types.					
29757379	9	53	theme	effect	1372:1377	arg1	example					1357:1363	a key example	1351:1363	a key example of the effect of glycosylation on Golgi exit	1351:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29757379	2	54	theme	related	234:240	arg1	activities					242:251	related activities	234:251	related activities	234:251	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	1	55	theme	bonds	179:183	arg1	formation					156:164	the formation	152:164	the formation of disulfide bonds in protein substrates	152:205	Quiescin sulfhydryl oxidase 1 (QSOX1) catalyzes the formation of disulfide bonds in protein substrates.					
29757379	3	56	theme	matrix	502:507	arg1	assembly					509:516	extracellular matrix assembly	488:516	extracellular matrix assembly	488:516	QSOX1 is upregulated in quiescent fibroblast cells and secreted into the extracellular environment, where it contributes to extracellular matrix assembly.					
29757379	5	57	theme	post-translational	762:779	arg1	modifications					781:793	post-translational modifications	762:793	post-translational modifications	762:793	To achieve a better understanding of factors that dictate QSOX1 localization and function, we aimed to determine how post-translational modifications affect QSOX1 trafficking and activity.					
29757379	0	58	gly	glycosite	38:46	arg2	glycosite					38:46	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation	0:55	Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization.					
29757379	2	59	theme	Golgi	335:339	arg1	apparatus					341:349	the Golgi apparatus	331:349	the Golgi apparatus	331:349	Unlike other enzymes with related activities, which are commonly found in the endoplasmic reticulum, QSOX1 is localized to the Golgi apparatus or secreted.					
29757379	9	60	theme	glycosylation	1382:1394	arg1	effect					1372:1377	the effect	1368:1377	the effect of glycosylation on Golgi exit	1368:1408	This study provides a key example of the effect of glycosylation on Golgi exit and contributes to an understanding of late secretory sorting and quality control.					
29333671	0	0	theme	hydrophilic	87:97	arg1	material					99:106	a dextran-modified hydrophilic material	68:106	a dextran-modified hydrophilic material	68:106	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	4	1	gly	glycoprotein	724:735	arg1	glycoprotein					724:735	α1 -acid glycoprotein	715:735	α1 -acid glycoprotein	715:735	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	1	2	theme	mass	178:181	arg1	approaches					202:211	mass spectrometry based approaches	178:211	mass spectrometry based approaches	178:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	0	3	theme	dextran-modified	70:85	arg1	material					99:106	a dextran-modified hydrophilic material	68:106	a dextran-modified hydrophilic material	68:106	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	0	4	link	N-linked	24:31	arg1	glycopeptides					33:45	N-linked glycopeptides	24:45	N-linked glycopeptides	24:45	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	1	5	theme	spectrometry	183:194	arg1	approaches					202:211	mass spectrometry based approaches	178:211	mass spectrometry based approaches	178:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	3	6	from	Sepharose	628:636	arg1	evaluation					656:665	glycoproteomic evaluation	641:665	glycoproteomic evaluation	641:665	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	1	7	from	sources	160:166	arg1	proteins					135:142	proteins	135:142	proteins from biological sources utilizing mass spectrometry based approaches	135:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	7	from	sources	160:166	arg1	analysis					123:130	Glycosylation analysis	109:130	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches	109:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	8	theme	structural	286:295	arg1	diversity					297:305	the structural diversity	282:305	the structural diversity of glycans	282:316	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	5	9	theme	glycoproteomics	1017:1031	arg1	fields					1007:1012	the fields	1003:1012	the fields of glycoproteomics and glycomics	1003:1045	Therefore, the dextran-bonded silica is expected to make contributions in the fields of glycoproteomics and glycomics.					
29333671	3	10	theme	commercial	617:626	arg1	Sepharose					628:636	commercial Sepharose	617:636	commercial Sepharose in glycoproteomic evaluation	617:665	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	1	11	gly	glycopeptides	267:279	arg2	glycopeptides					267:279	glycopeptides	267:279	glycopeptides	267:279	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	11	gly	glycopeptides	267:279	arg1	glycans					310:316	glycans	310:316	glycans	310:316	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	2	12	theme	customized	365:374	arg1	phase					415:419	a customized dextran-bonded silica-based stationary phase	363:419	a customized dextran-bonded silica-based stationary phase	363:419	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	1	13	theme	diversity	297:305	arg1	abundance					254:262	the relatively low abundance	235:262	the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices	235:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	5	14	from	contributions	986:998	arg1	fields					1007:1012	the fields	1003:1012	the fields of glycoproteomics and glycomics	1003:1045	Therefore, the dextran-bonded silica is expected to make contributions in the fields of glycoproteomics and glycomics.					
29333671	4	15	theme	fetuin	707:712	arg1	analysis					695:702	the glycomic analysis	682:702	the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome	682:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	3	16	theme	glycosylation	584:596	arg1	coverage					603:610	broader glycosylation site coverage	576:610	broader glycosylation site coverage	576:610	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	1	17	theme	based	196:200	arg1	approaches					202:211	mass spectrometry based approaches	178:211	mass spectrometry based approaches	178:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	2	18	theme	complex	495:501	arg1	samples					514:520	complex biological samples	495:520	complex biological samples	495:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	2	19	theme	glycans	482:488	arg1	enrichment					450:459	selective enrichment	440:459	selective enrichment of glycopeptides and glycans from complex biological samples	440:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	4	20	theme	glycomic	686:693	arg1	analysis					695:702	the glycomic analysis	682:702	the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome	682:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	4	21	theme	glycoprotein	724:735	arg1	analysis					695:702	the glycomic analysis	682:702	the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome	682:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	2	22	from	samples	514:520	arg1	enrichment					450:459	selective enrichment	440:459	selective enrichment of glycopeptides and glycans from complex biological samples	440:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	5	23	theme	glycomics	1037:1045	arg1	fields					1007:1012	the fields	1003:1012	the fields of glycoproteomics and glycomics	1003:1045	Therefore, the dextran-bonded silica is expected to make contributions in the fields of glycoproteomics and glycomics.					
29333671	4	24	theme	human	742:746	arg1	N-glycome					754:762	human serum N-glycome	742:762	human serum N-glycome	742:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	0	25	theme	Selective	0:8	arg1	enrichment					10:19	Selective enrichment	0:19	Selective enrichment of N-linked glycopeptides and glycans	0:57	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	4	26	theme	higher	794:799	arg1	sensitivity					801:811	the relatively higher sensitivity	779:811	the relatively higher sensitivity	779:811	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	3	27	theme	glycoproteomic	641:654	arg1	evaluation					656:665	glycoproteomic evaluation	641:665	glycoproteomic evaluation	641:665	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	2	28	theme	stationary	404:413	arg1	phase					415:419	a customized dextran-bonded silica-based stationary phase	363:419	a customized dextran-bonded silica-based stationary phase	363:419	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	0	29	theme	N-linked	24:31	arg1	glycopeptides					33:45	N-linked glycopeptides	24:45	N-linked glycopeptides	24:45	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	1	30	theme	glycans	310:316	arg1	glycopeptides					267:279	glycopeptides	267:279	glycopeptides	267:279	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	30	theme	glycans	310:316	arg1	matrices					338:345	the coexisting matrices	323:345	the coexisting matrices	323:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	30	theme	glycans	310:316	arg1	diversity					297:305	the structural diversity	282:305	the structural diversity of glycans	282:316	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	31	theme	Glycosylation	109:121	arg1	analysis					123:130	Glycosylation analysis	109:130	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches	109:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	2	32	theme	glycopeptides	464:476	arg1	enrichment					450:459	selective enrichment	440:459	selective enrichment of glycopeptides and glycans from complex biological samples	440:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	4	33	theme	dextran-bonded	853:866	arg1	silica					868:873	dextran-bonded silica	853:873	dextran-bonded silica	853:873	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	3	34	gly	glycosylation	584:596	arg2	site					598:601	broader glycosylation site coverage	576:610	broader glycosylation site coverage	576:610	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	3	34	gly	glycosylation	584:596	arg2	coverage					603:610	broader glycosylation site coverage	576:610	broader glycosylation site coverage	576:610	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	4	35	theme	N-glycome	754:762	arg1	analysis					695:702	the glycomic analysis	682:702	the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome	682:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	3	36	theme	superior	551:558	arg1	selectivity					560:570	superior selectivity	551:570	superior selectivity	551:570	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	4	37	theme	-acid	718:722	arg1	glycoprotein					724:735	α1 -acid glycoprotein	715:735	α1 -acid glycoprotein	715:735	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	2	38	theme	selective	440:448	arg1	enrichment					450:459	selective enrichment	440:459	selective enrichment of glycopeptides and glycans from complex biological samples	440:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	0	39	theme	glycopeptides	33:45	arg1	enrichment					10:19	Selective enrichment	0:19	Selective enrichment of N-linked glycopeptides and glycans	0:57	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	5	40	theme	dextran-bonded	944:957	arg1	silica					959:964	the dextran-bonded silica	940:964	the dextran-bonded silica	940:964	Therefore, the dextran-bonded silica is expected to make contributions in the fields of glycoproteomics and glycomics.					
29333671	4	41	theme	glycoform	831:839	arg1	coverage					841:848	glycoform coverage	831:848	glycoform coverage	831:848	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	4	42	theme	α1	715:716	arg1	glycoprotein					724:735	α1 -acid glycoprotein	715:735	α1 -acid glycoprotein	715:735	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	2	43	theme	biological	503:512	arg1	samples					514:520	complex biological samples	495:520	complex biological samples	495:520	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	1	44	theme	coexisting	327:336	arg1	matrices					338:345	the coexisting matrices	323:345	the coexisting matrices	323:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	4	45	theme	graphitized	909:919	arg1	carbon					921:926	porous graphitized carbon	902:926	porous graphitized carbon	902:926	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	0	46	theme	glycans	51:57	arg1	enrichment					10:19	Selective enrichment	0:19	Selective enrichment of N-linked glycopeptides and glycans	0:57	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	1	47	theme	matrices	338:345	arg1	abundance					254:262	the relatively low abundance	235:262	the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices	235:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	4	48	theme	porous	902:907	arg1	carbon					921:926	porous graphitized carbon	902:926	porous graphitized carbon	902:926	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	1	49	theme	proteins	135:142	arg1	analysis					123:130	Glycosylation analysis	109:130	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches	109:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	0	50	gly	glycopeptides	33:45	arg2	glycopeptides					33:45	N-linked glycopeptides	24:45	N-linked glycopeptides	24:45	Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material.					
29333671	4	51	theme	serum	748:752	arg1	N-glycome					754:762	human serum N-glycome	742:762	human serum N-glycome	742:762	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	1	52	from	analysis	123:130	arg1	sources					160:166	biological sources	149:166	biological sources utilizing mass spectrometry based approaches	149:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	1	53	theme	low	250:252	arg1	abundance					254:262	the relatively low abundance	235:262	the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices	235:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	3	54	theme	site	598:601	arg1	coverage					603:610	broader glycosylation site coverage	576:610	broader glycosylation site coverage	576:610	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	4	55	theme	silica	868:873	arg1	coverage					841:848	glycoform coverage	831:848	glycoform coverage	831:848	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	4	55	theme	silica	868:873	arg1	selectivity					814:824	selectivity	814:824	selectivity	814:824	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	4	55	theme	silica	868:873	arg1	sensitivity					801:811	the relatively higher sensitivity	779:811	the relatively higher sensitivity	779:811	Additionally, the glycomic analysis of fetuin, α1 -acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon.					
29333671	1	56	theme	biological	149:158	arg1	sources					160:166	biological sources	149:166	biological sources utilizing mass spectrometry based approaches	149:211	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
29333671	2	57	gly	glycopeptides	464:476	arg2	glycopeptides					464:476	glycopeptides	464:476	glycopeptides	464:476	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	2	58	theme	silica-based	391:402	arg1	phase					415:419	a customized dextran-bonded silica-based stationary phase	363:419	a customized dextran-bonded silica-based stationary phase	363:419	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	3	59	theme	broader	576:582	arg1	coverage					603:610	broader glycosylation site coverage	576:610	broader glycosylation site coverage	576:610	This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation.					
29333671	2	60	theme	dextran-bonded	376:389	arg1	phase					415:419	a customized dextran-bonded silica-based stationary phase	363:419	a customized dextran-bonded silica-based stationary phase	363:419	In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples.					
29333671	1	61	theme	glycopeptides	267:279	arg1	abundance					254:262	the relatively low abundance	235:262	the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices	235:345	Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices.					
30078634	6	0	theme	protein	972:978	arg1	response					980:987	the cellular unfolded protein response	950:987	the cellular unfolded protein response	950:987	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	1	theme	unfolded	963:970	arg1	response					980:987	the cellular unfolded protein response	950:987	the cellular unfolded protein response	950:987	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	3	2	theme	analogs	524:530	arg1	activity					498:505	the activity	494:505	the activity of NGI-1 and its analogs	494:530	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	4	3	theme	STT3A	581:585	arg1	function					564:571	the function	560:571	the function of both STT3A and STT3B	560:595	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	6	4	theme	cellular	954:961	arg1	response					980:987	the cellular unfolded protein response	950:987	the cellular unfolded protein response	950:987	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	4	5	theme	inhibitors	687:696	arg1	synthesis					647:655	synthesis	647:655	synthesis of catalytic subunit-specific inhibitors	647:696	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	3	6	theme	cell	411:414	arg1	models					416:421	cell models	411:421	cell models	411:421	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	6	7	theme	biological	860:869	arg1	effects					871:877	discrete biological effects	851:877	discrete biological effects on endogenous STT3B target proteins such as COX2	851:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	4	8	theme	STT3B	591:595	arg1	function					564:571	the function	560:571	the function of both STT3A and STT3B	560:595	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	1	9	theme	multisubunit	131:142	arg1	complex					151:157	a multisubunit enzyme complex	129:157	a multisubunit enzyme complex	129:157	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	1	9	theme	multisubunit	131:142	arg1	oligosaccharyltransferase					94:118	The oligosaccharyltransferase	90:118	The oligosaccharyltransferase (OST)	90:124	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	1	9	theme	multisubunit	131:142	arg1	constitutive					237:248	constitutive	237:248	constitutive	237:248	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	6	10	theme	discrete	851:858	arg1	effects					871:877	discrete biological effects	851:877	discrete biological effects on endogenous STT3B target proteins such as COX2	851:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	7	11	link	N-linked	1105:1112	arg1	glycosylation					1114:1126	N-linked glycosylation	1105:1126	N-linked glycosylation	1105:1126	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30078634	1	12	theme	secretory	195:203	arg1	pathway					205:211	the secretory pathway	191:211	the secretory pathway	191:211	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	0	13	theme	Oligosaccharyltransferase	52:76	arg1	Inhibitors					78:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.					
30078634	0	14	theme	Small-Molecule	37:50	arg1	Inhibitors					78:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.					
30078634	1	15	from	complex	151:157	arg1	pathway					205:211	the secretory pathway	191:211	the secretory pathway	191:211	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	4	16	theme	subunit-specific	670:685	arg1	inhibitors					687:696	catalytic subunit-specific inhibitors	660:696	catalytic subunit-specific inhibitors	660:696	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	0	17	theme	N-Glycan	8:15	arg1	Occupancy					22:30	N-Glycan Site Occupancy	8:30	N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors	8:87	Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.					
30078634	3	18	dep	subunit	459:465	arg1	STT3B					477:481	STT3B	477:481	STT3B	477:481	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	3	18	dep	subunit	459:465	arg1	STT3A					468:472	STT3A	468:472	STT3A	468:472	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	6	19	from	effects	871:877	arg1	proteins					906:913	endogenous STT3B target proteins	882:913	endogenous STT3B target proteins such as COX2	882:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	19	from	effects	871:877	arg1	COX2					923:926	COX2	923:926	COX2	923:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	2	20	theme	pharmacological	330:344	arg1	approach					346:353	a pharmacological approach	328:353	a pharmacological approach for regulating N-linked glycosylation	328:391	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	0	21	theme	Site	17:20	arg1	Occupancy					22:30	N-Glycan Site Occupancy	8:30	N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors	8:87	Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.					
30078634	3	22	theme	catalytic	449:457	arg1	subunit					459:465	OST catalytic subunit	445:465	each OST catalytic subunit (STT3A or STT3B)	440:482	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	7	23	gly	glycoproteins	1038:1050	arg1	glycoproteins					1038:1050	glycoproteins	1038:1050	glycoproteins	1038:1050	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30078634	5	24	theme	STT3B	749:753	arg1	selectivity					755:765	STT3B selectivity	749:765	STT3B selectivity	749:765	Using this approach, pharmacophores that increase STT3B selectivity are characterized and an STT3B-specific inhibitor is identified.					
30078634	4	25	theme	catalytic	660:668	arg1	inhibitors					687:696	catalytic subunit-specific inhibitors	660:696	catalytic subunit-specific inhibitors	660:696	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	3	26	theme	subunit	459:465	arg1	knockout					428:435	knockout	428:435	knockout	428:435	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	0	27	with	Occupancy	22:30	arg1	Inhibitors					78:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Small-Molecule Oligosaccharyltransferase Inhibitors	37:87	Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.					
30078634	6	28	contain	has	847:849	arg1	inhibitor					837:845	This inhibitor	832:845	This inhibitor	832:845	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	28	contain	has	847:849	arg2	effects					871:877	discrete biological effects	851:877	discrete biological effects on endogenous STT3B target proteins such as COX2	851:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	3	29	theme	NGI-1	510:514	arg1	activity					498:505	the activity	494:505	the activity of NGI-1 and its analogs	494:530	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	3	30	theme	OST	445:447	arg1	subunit					459:465	OST catalytic subunit	445:465	each OST catalytic subunit (STT3A or STT3B)	440:482	Herein we design cell models with knockout of each OST catalytic subunit (STT3A or STT3B) to screen the activity of NGI-1 and its analogs.					
30078634	1	31	theme	enzyme	144:149	arg1	complex					151:157	a multisubunit enzyme complex	129:157	a multisubunit enzyme complex	129:157	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	1	31	theme	enzyme	144:149	arg1	oligosaccharyltransferase					94:118	The oligosaccharyltransferase	90:118	The oligosaccharyltransferase (OST)	90:124	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	1	31	theme	enzyme	144:149	arg1	constitutive					237:248	constitutive	237:248	constitutive	237:248	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	7	32	theme	N-linked	1105:1112	arg1	glycosylation					1114:1126	N-linked glycosylation	1105:1126	N-linked glycosylation	1105:1126	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30078634	6	33	theme	target	899:904	arg1	proteins					906:913	endogenous STT3B target proteins	882:913	endogenous STT3B target proteins such as COX2	882:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	33	theme	target	899:904	arg1	COX2					923:926	COX2	923:926	COX2	923:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	7	34	theme	pharmacologic	1077:1089	arg1	inhibition					1091:1100	pharmacologic inhibition	1077:1100	pharmacologic inhibition of N-linked glycosylation	1077:1126	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30078634	2	35	theme	N-linked	370:377	arg1	glycosylation					379:391	N-linked glycosylation	370:391	N-linked glycosylation	370:391	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	4	36	theme	structure-activity	605:622	arg1	relationships					624:636	structure-activity relationships	605:636	structure-activity relationships	605:636	We show that NGI-1 targets the function of both STT3A and STT3B and use structure-activity relationships to guide synthesis of catalytic subunit-specific inhibitors.					
30078634	7	37	theme	glycosylation	1114:1126	arg1	inhibition					1091:1100	pharmacologic inhibition	1077:1100	pharmacologic inhibition of N-linked glycosylation	1077:1126	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30078634	6	38	theme	STT3B	893:897	arg1	proteins					906:913	endogenous STT3B target proteins	882:913	endogenous STT3B target proteins such as COX2	882:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	38	theme	STT3B	893:897	arg1	COX2					923:926	COX2	923:926	COX2	923:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	2	39	theme	OST	291:293	arg1	NGI-1					314:318	NGI-1	314:318	NGI-1	314:318	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	2	39	theme	OST	291:293	arg1	inhibitors					295:304	small-molecule OST inhibitors	276:304	small-molecule OST inhibitors such as NGI-1	276:318	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	5	40	theme	STT3B-specific	792:805	arg1	inhibitor					807:815	an STT3B-specific inhibitor	789:815	an STT3B-specific inhibitor	789:815	Using this approach, pharmacophores that increase STT3B selectivity are characterized and an STT3B-specific inhibitor is identified.					
30078634	6	41	theme	endogenous	882:891	arg1	proteins					906:913	endogenous STT3B target proteins	882:913	endogenous STT3B target proteins such as COX2	882:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	6	41	theme	endogenous	882:891	arg1	COX2					923:926	COX2	923:926	COX2	923:926	This inhibitor has discrete biological effects on endogenous STT3B target proteins such as COX2 but does not activate the cellular unfolded protein response.					
30078634	2	42	theme	small-molecule	276:289	arg1	NGI-1					314:318	NGI-1	314:318	NGI-1	314:318	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	2	42	theme	small-molecule	276:289	arg1	inhibitors					295:304	small-molecule OST inhibitors	276:304	small-molecule OST inhibitors such as NGI-1	276:318	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	1	43	theme	N-glycosylates	164:177	arg1	proteins					179:186	N-glycosylates proteins	164:186	N-glycosylates proteins	164:186	The oligosaccharyltransferase (OST) is a multisubunit enzyme complex that N-glycosylates proteins in the secretory pathway and is considered to be constitutive and unregulated.					
30078634	2	44	link	N-linked	370:377	arg1	glycosylation					379:391	N-linked glycosylation	370:391	N-linked glycosylation	370:391	However, small-molecule OST inhibitors such as NGI-1 provide a pharmacological approach for regulating N-linked glycosylation.					
30078634	7	45	theme	glycoproteins	1038:1050	arg1	subsets					1027:1033	subsets	1027:1033	subsets of glycoproteins	1027:1050	Together this work demonstrates that subsets of glycoproteins can be regulated through pharmacologic inhibition of N-linked glycosylation.					
30144627	7	0	theme	pregnant	1318:1325	arg1	women					1327:1331	pregnant women	1318:1331	pregnant women	1318:1331	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	2	1	theme	glycosylation	525:537	arg1	sites					539:543	8 glycosylation sites	523:543	8 glycosylation sites leading to a high number of isoforms	523:580	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	6	2	theme	retention	1132:1140	arg1	times					1142:1146	retention times	1132:1146	retention times	1132:1146	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	11	3	theme	N-glycans	1756:1764	arg1	weights					1741:1747	the molecular weights	1727:1747	the molecular weights of the N-glycans already described in the literature for hCG	1727:1808	Then, the molecular weights of the N-glycans already described in the literature for hCG were compiled in a database to identify the hCGα glycoforms by mass matching.					
30144627	1	4	theme	intact	250:255	arg1	level					257:261	the intact level	246:261	the intact level	246:261	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	7	5	dep	drugs	1232:1236	arg1	drugs					1232:1236	two hCG-based drugs	1218:1236	two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG)	1218:1339	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	5	dep	drugs	1232:1236	arg1	Pregnyl®					1281:1288	Pregnyl®	1281:1288	Pregnyl® (hCG isolated from urine of pregnant women, u-hCG)	1281:1339	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	5	dep	drugs	1232:1236	arg1	Ovitrelle®					1239:1248	Ovitrelle®	1239:1248	Ovitrelle® (a recombinant hCG, r-hCG)	1239:1275	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	9	6	theme	dramatic	1468:1475	arg1	heterogeneity					1484:1496	its dramatic higher heterogeneity	1464:1496	its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source	1464:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	7	7	theme	recombinant	1253:1263	arg1	r-hCG					1270:1274	r-hCG	1270:1274	r-hCG	1270:1274	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	7	theme	recombinant	1253:1263	arg1	hCG					1265:1267	a recombinant hCG	1251:1267	a recombinant hCG	1251:1267	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	2	8	gly	glycosylation	525:537	arg2	8					523:523	8	523:523	8	523:523	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	2	8	gly	glycosylation	525:537	arg2	sites					539:543	8 glycosylation sites	523:543	8 glycosylation sites leading to a high number of isoforms	523:580	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	6	9	theme	method	1113:1118	arg1	repeatability					1086:1098	The repeatability	1082:1098	The repeatability of the final method in terms of retention times and peak areas	1082:1161	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	9	10	gly	glycosylation	1515:1527	arg2	sites					1529:1533	6 glycosylation sites	1513:1533	6 glycosylation sites	1513:1533	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	10	gly	glycosylation	1515:1527	arg2	ionization					1549:1558	ionization	1549:1558	ionization	1549:1558	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	10	gly	glycosylation	1515:1527	arg2	6					1513:1513	6	1513:1513	6	1513:1513	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	10	gly	glycosylation	1515:1527	arg1	ionization					1549:1558	ionization	1549:1558	ionization	1549:1558	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	7	11	dep	Ovitrelle®	1239:1248	arg1	r-hCG					1270:1274	r-hCG	1270:1274	r-hCG	1270:1274	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	11	dep	Ovitrelle®	1239:1248	arg1	hCG					1265:1267	a recombinant hCG	1251:1267	a recombinant hCG	1251:1267	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	3	12	theme	isoforms	652:659	arg1	number					642:647	the largest number	630:647	the largest number of isoforms	630:659	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	0	13	theme	high-resolution	121:135	arg1	spectrometry					142:153	high-resolution mass spectrometry	121:153	high-resolution mass spectrometry at the intact level	121:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	6	14	theme	final	1107:1111	arg1	method					1113:1118	the final method	1103:1118	the final method	1103:1118	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	3	15	theme	MS	688:689	arg1	process					702:708	the MS ionization process	684:708	the MS ionization process	684:708	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	1	16	theme	Chorionic	206:214	arg1	hCG					230:232	hCG	230:232	hCG	230:232	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	16	theme	Chorionic	206:214	arg1	Gonadotropin					216:227	the human Chorionic Gonadotropin	196:227	the human Chorionic Gonadotropin (hCG) hormone at the intact level	196:261	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	4	17	theme	column	795:800	arg1	temperature					802:812	column temperature	795:812	column temperature	795:812	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	7	18	theme	hCG-based	1222:1230	arg1	drugs					1232:1236	two hCG-based drugs	1218:1236	two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG)	1218:1339	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	18	theme	hCG-based	1222:1230	arg1	Pregnyl®					1281:1288	Pregnyl®	1281:1288	Pregnyl® (hCG isolated from urine of pregnant women, u-hCG)	1281:1339	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	18	theme	hCG-based	1222:1230	arg1	Ovitrelle®					1239:1248	Ovitrelle®	1239:1248	Ovitrelle® (a recombinant hCG, r-hCG)	1239:1275	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	6	19	from	repeatability	1086:1098	arg1	terms					1123:1127	terms	1123:1127	terms of retention times and peak areas	1123:1161	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	3	20	theme	data	714:717	arg1	treatment					719:727	data treatment	714:727	data treatment	714:727	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	6	21	theme	areas	1157:1161	arg1	terms					1123:1127	terms	1123:1127	terms of retention times and peak areas	1123:1161	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	11	22	theme	molecular	1731:1739	arg1	weights					1741:1747	the molecular weights	1727:1747	the molecular weights of the N-glycans already described in the literature for hCG	1727:1808	Then, the molecular weights of the N-glycans already described in the literature for hCG were compiled in a database to identify the hCGα glycoforms by mass matching.					
30144627	13	23	theme	first	2026:2030	arg1	time					2032:2035	the first time	2022:2035	the first time	2022:2035	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	8	24	theme	analytical	1376:1385	arg1	method					1387:1392	the analytical method	1372:1392	the analytical method	1372:1392	After the deconvolution step, the analytical method did not allow to observe the isoforms of the hCGβ.					
30144627	0	25	theme	reversed	71:78	arg1	chromatography					93:106	reversed phase liquid chromatography	71:106	reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level	71:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	2	26	theme	so-called	476:484	arg1	subunit					467:473	a β subunit	463:473	a β subunit	463:473	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	2	26	theme	so-called	476:484	arg1	hCGα					486:489	so-called hCGα	476:489	so-called hCGα	476:489	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	0	27	theme	liquid	86:91	arg1	chromatography					93:106	reversed phase liquid chromatography	71:106	reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level	71:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	5	28	theme	ions	993:996	arg1	transfer					968:975	the efficient transfer	954:975	the efficient transfer of the heaviest ions	954:996	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	4	29	theme	separated	868:876	arg1	isoforms					878:885	separated isoforms	868:885	separated isoforms	868:885	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	8	30	theme	hCGβ	1439:1442	arg1	isoforms					1423:1430	the isoforms	1419:1430	the isoforms of the hCGβ	1419:1442	After the deconvolution step, the analytical method did not allow to observe the isoforms of the hCGβ.					
30144627	1	31	theme	resolution	346:355	arg1	HRMS					376:379	HRMS	376:379	HRMS	376:379	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	31	theme	resolution	346:355	arg1	spectrometry					362:373	high resolution mass spectrometry	341:373	high resolution mass spectrometry (HRMS)	341:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	2	32	theme	human	425:429	arg1	pregnancy					431:439	human pregnancy	425:439	human pregnancy	425:439	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	12	33	theme	glycoforms	1958:1967	arg1	identification					1935:1948	the identification	1931:1948	the identification of five glycoforms for both r-hCG and u-hCG	1931:1992	This strategy was successfully applied for the identification of five glycoforms for both r-hCG and u-hCG.					
30144627	2	34	contain	has	519:521	arg2	sites					539:543	8 glycosylation sites	523:543	8 glycosylation sites leading to a high number of isoforms	523:580	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	2	34	contain	has	519:521	arg1	protein					402:408	This heterodimeric protein	383:408	This heterodimeric protein	383:408	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	7	35	dep	Pregnyl®	1281:1288	arg1	hCG					1291:1293	hCG	1291:1293	hCG isolated from urine of pregnant women	1291:1331	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	35	dep	Pregnyl®	1281:1288	arg1	u-hCG					1334:1338	u-hCG	1334:1338	u-hCG	1334:1338	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	1	36	theme	reversed	284:291	arg1	RPLC					322:325	RPLC	322:325	RPLC	322:325	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	36	theme	reversed	284:291	arg1	chromatography					306:319	reversed phase liquid chromatography	284:319	reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS)	284:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	4	37	theme	mobile	742:747	arg1	composition					755:765	The initial mobile phase composition	730:765	The initial mobile phase composition	730:765	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	7	38	attach	isolated	1295:1302	arg1	urine					1309:1313	urine	1309:1313	urine of pregnant women	1309:1331	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	38	attach	isolated	1295:1302	arg2	u-hCG					1334:1338	u-hCG	1334:1338	u-hCG	1334:1338	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	7	38	attach	isolated	1295:1302	arg2	hCG					1291:1293	hCG	1291:1293	hCG isolated from urine of pregnant women	1291:1331	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	0	39	theme	Chorionic	37:45	arg1	Gonadotropin					47:58	human Chorionic Gonadotropin	31:58	the human Chorionic Gonadotropin protein	27:66	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	1	40	theme	liquid	299:304	arg1	RPLC					322:325	RPLC	322:325	RPLC	322:325	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	40	theme	liquid	299:304	arg1	chromatography					306:319	reversed phase liquid chromatography	284:319	reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS)	284:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	4	41	theme	phase	749:753	arg1	composition					755:765	The initial mobile phase composition	730:765	The initial mobile phase composition	730:765	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	1	42	theme	Gonadotropin	216:227	arg1	hormone					235:241	the human Chorionic Gonadotropin (hCG) hormone	196:241	the human Chorionic Gonadotropin (hCG) hormone at the intact level	196:261	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	7	43	theme	women	1327:1331	arg1	urine					1309:1313	urine	1309:1313	urine of pregnant women	1309:1331	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	5	44	theme	MS	902:903	arg1	parameters					915:924	the MS detection parameters	898:924	the MS detection parameters	898:924	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	13	45	gly	glycoforms	2106:2115	arg1	hCGα					2101:2104	the intact hCGα glycoforms	2090:2115	the intact hCGα glycoforms	2090:2115	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	10	46	theme	hCGα	1642:1645	arg1	isoforms					1647:1654	more than 30 hCGα isoforms	1629:1654	more than 30 hCGα isoforms	1629:1654	Nevertheless, the results revealed the presence of more than 30 hCGα isoforms, which differ by their number and their nature in the two drugs.					
30144627	13	47	theme	hCGα	2101:2104	arg1	glycoforms					2106:2115	the intact hCGα glycoforms	2090:2115	the intact hCGα glycoforms	2090:2115	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	6	48	theme	peak	1152:1155	arg1	areas					1157:1161	peak areas	1152:1161	peak areas	1152:1161	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	5	49	dep	improve	1057:1063	arg1	iii					1052:1054	iii	1052:1054	iii	1052:1054	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	11	50	theme	hCGα	1854:1857	arg1	glycoforms					1859:1868	the hCGα glycoforms	1850:1868	the hCGα glycoforms	1850:1868	Then, the molecular weights of the N-glycans already described in the literature for hCG were compiled in a database to identify the hCGα glycoforms by mass matching.					
30144627	0	51	from	level	169:173	arg1	spectrometry					142:153	high-resolution mass spectrometry	121:153	high-resolution mass spectrometry at the intact level	121:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	6	52	theme	times	1142:1146	arg1	terms					1123:1127	terms	1123:1127	terms of retention times and peak areas	1123:1161	The repeatability of the final method in terms of retention times and peak areas was assessed.					
30144627	4	53	theme	gradient	781:788	arg1	temperature					802:812	column temperature	795:812	column temperature	795:812	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	4	53	theme	gradient	781:788	arg1	slope					768:772	slope	768:772	slope of the gradient	768:788	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	4	53	theme	gradient	781:788	arg1	composition					755:765	The initial mobile phase composition	730:765	The initial mobile phase composition	730:765	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	5	54	dep	promote	946:952	arg1	i					943:943	i	943:943	i	943:943	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	13	55	theme	RPLC-HRMS	2054:2062	arg1	potential					2041:2049	the potential	2037:2049	the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms	2037:2115	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	2	56	theme	high	558:561	arg1	number					563:568	a high number	556:568	a high number of isoforms	556:580	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	3	57	theme	largest	634:640	arg1	number					642:647	the largest number	630:647	the largest number of isoforms	630:659	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	1	58	from	level	257:261	arg1	hormone					235:241	the human Chorionic Gonadotropin (hCG) hormone	196:241	the human Chorionic Gonadotropin (hCG) hormone at the intact level	196:261	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	59	theme	first	184:188	arg1	time					190:193	the first time	180:193	the first time	180:193	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	9	60	theme	higher	1477:1482	arg1	heterogeneity					1484:1496	its dramatic higher heterogeneity	1464:1496	its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source	1464:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	0	61	theme	mass	137:140	arg1	spectrometry					142:153	high-resolution mass spectrometry	121:153	high-resolution mass spectrometry at the intact level	121:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	1	62	theme	human	200:204	arg1	hCG					230:232	hCG	230:232	hCG	230:232	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	62	theme	human	200:204	arg1	Gonadotropin					216:227	the human Chorionic Gonadotropin	196:227	the human Chorionic Gonadotropin (hCG) hormone at the intact level	196:261	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	3	63	theme	ionization	691:700	arg1	process					702:708	the MS ionization process	684:708	the MS ionization process	684:708	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	0	64	theme	intact	162:167	arg1	level					169:173	the intact level	158:173	the intact level	158:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	5	65	theme	heaviest	984:991	arg1	ions					993:996	the heaviest ions	980:996	the heaviest ions	980:996	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	9	66	theme	glycosylation	1515:1527	arg1	ionization					1549:1558	ionization	1549:1558	ionization	1549:1558	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	66	theme	glycosylation	1515:1527	arg1	sites					1529:1533	6 glycosylation sites	1513:1533	6 glycosylation sites	1513:1533	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	67	from	sites	1529:1533	arg1	source					1570:1575	the MS source	1563:1575	the MS source	1563:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	5	68	theme	efficient	958:966	arg1	transfer					968:975	the efficient transfer	954:975	the efficient transfer of the heaviest ions	954:996	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	4	69	theme	isoforms	878:885	arg1	number					858:863	the number	854:863	the number of separated isoforms	854:885	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	7	70	used	used	1197:1200	arg2	method					1181:1186	The method	1177:1186	The method	1177:1186	The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG).					
30144627	0	71	theme	phase	80:84	arg1	chromatography					93:106	reversed phase liquid chromatography	71:106	reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level	71:173	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	9	72	theme	ionization	1549:1558	arg1	ionization					1549:1558	ionization	1549:1558	ionization	1549:1558	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	72	theme	ionization	1549:1558	arg1	sites					1529:1533	6 glycosylation sites	1513:1533	6 glycosylation sites	1513:1533	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	9	72	theme	ionization	1549:1558	arg1	lack					1541:1544	a lack	1539:1544	a lack of ionization in the MS source	1539:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	2	73	theme	isoforms	573:580	arg1	number					563:568	a high number	556:568	a high number of isoforms	556:580	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	1	74	theme	high	341:344	arg1	HRMS					376:379	HRMS	376:379	HRMS	376:379	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	74	theme	high	341:344	arg1	spectrometry					362:373	high resolution mass spectrometry	341:373	high resolution mass spectrometry (HRMS)	341:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	2	75	theme	β	465:465	arg1	hCGβ					495:498	hCGβ	495:498	hCGβ	495:498	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	2	75	theme	β	465:465	arg1	subunit					467:473	a β subunit	463:473	a β subunit	463:473	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	2	75	theme	β	465:465	arg1	hCGα					486:489	so-called hCGα	476:489	so-called hCGα	476:489	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	8	76	theme	deconvolution	1352:1364	arg1	step					1366:1369	the deconvolution step	1348:1369	the deconvolution step	1348:1369	After the deconvolution step, the analytical method did not allow to observe the isoforms of the hCGβ.					
30144627	9	77	theme	MS	1567:1568	arg1	source					1570:1575	the MS source	1563:1575	the MS source	1563:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	1	78	theme	mass	357:360	arg1	HRMS					376:379	HRMS	376:379	HRMS	376:379	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	78	theme	mass	357:360	arg1	spectrometry					362:373	high resolution mass spectrometry	341:373	high resolution mass spectrometry (HRMS)	341:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	13	79	theme	intact	2094:2099	arg1	glycoforms					2106:2115	the intact hCGα glycoforms	2090:2115	the intact hCGα glycoforms	2090:2115	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	5	80	dep	avoid	1003:1007	arg1	ii					999:1000	ii	999:1000	ii	999:1000	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	2	81	theme	heterodimeric	388:400	arg1	protein					402:408	This heterodimeric protein	383:408	This heterodimeric protein	383:408	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	0	82	theme	human	31:35	arg1	Gonadotropin					47:58	human Chorionic Gonadotropin	31:58	the human Chorionic Gonadotropin protein	27:66	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	13	83	theme	glycoforms	2106:2115	arg1	identification					2072:2085	the identification	2068:2085	the identification of the intact hCGα glycoforms	2068:2115	This work demonstrates for the first time the potential of RPLC-HRMS for the identification of the intact hCGα glycoforms.					
30144627	0	84	theme	Gonadotropin	47:58	arg1	protein					60:66	the human Chorionic Gonadotropin protein	27:66	the human Chorionic Gonadotropin protein	27:66	An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level.					
30144627	1	85	theme	phase	293:297	arg1	RPLC					322:325	RPLC	322:325	RPLC	322:325	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	1	85	theme	phase	293:297	arg1	chromatography					306:319	reversed phase liquid chromatography	284:319	reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS)	284:380	For the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS).					
30144627	2	86	dep	α	457:457	arg1	an					454:455	an	454:455	an	454:455	This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms.					
30144627	3	87	theme	LC	594:595	arg1	method					597:602	the LC method	590:602	the LC method	590:602	First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment.					
30144627	4	88	theme	initial	734:740	arg1	composition					755:765	The initial mobile phase composition	730:765	The initial mobile phase composition	730:765	The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms.					
30144627	5	89	theme	ions	1043:1046	arg1	fragmentation					1022:1034	the fragmentation	1018:1034	the fragmentation of the ions	1018:1046	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30144627	10	90	theme	isoforms	1647:1654	arg1	presence					1617:1624	the presence	1613:1624	the presence of more than 30 hCGα isoforms, which differ by their number and their nature in the two drugs	1613:1718	Nevertheless, the results revealed the presence of more than 30 hCGα isoforms, which differ by their number and their nature in the two drugs.					
30144627	9	91	from	lack	1541:1544	arg1	source					1570:1575	the MS source	1563:1575	the MS source	1563:1575	This may be due to its dramatic higher heterogeneity induced by its 6 glycosylation sites and a lack of ionization in the MS source.					
30144627	5	92	theme	detection	905:913	arg1	parameters					915:924	the MS detection parameters	898:924	the MS detection parameters	898:924	Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity.					
30152690	4	0	theme	molecular	711:719	arg1	modeling					721:728	molecular modeling	711:728	molecular modeling	711:728	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	3	1	theme	catalyzed	514:522	arg1	loss					524:527	nonenzyme catalyzed loss	504:527	nonenzyme catalyzed loss of a complete N-linked glycan	504:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	5	2	theme	glycan	918:923	arg1	loss					925:928	The glycan loss	914:928	The glycan loss	914:928	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	6	3	theme	similar	1107:1113	arg1	mechanism					1129:1137	a similar but different mechanism	1105:1137	a similar but different mechanism than that of conventional deamidation	1105:1175	Further study indicated that the glycan shedding followed a similar but different mechanism than that of conventional deamidation.					
30152690	7	4	contain	has	1404:1406	arg2	impact					1411:1416	an impact	1408:1416	an impact on efficacy or safety	1408:1438	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	4	contain	has	1404:1406	arg1	glycosylation					1390:1402	glycosylation	1390:1402	glycosylation	1390:1402	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	5	theme	recombinant	1233:1243	arg1	proteins					1257:1264	many recombinant therapeutic proteins	1228:1264	many recombinant therapeutic proteins	1228:1264	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	3	6	theme	protein	474:480	arg1	degradation					482:492	a unique protein degradation	465:492	a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	465:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	4	7	theme	glycosylation	899:911	arg1	site					891:894	the site	887:894	the site of glycosylation	887:911	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	4	7	theme	glycosylation	899:911	arg1	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	3	8	theme	glycan	552:557	arg1	loss					524:527	nonenzyme catalyzed loss	504:527	nonenzyme catalyzed loss of a complete N-linked glycan	504:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	9	link	N-linked	543:550	arg1	glycan					552:557	a complete N-linked glycan	532:557	a complete N-linked glycan	532:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	5	10	theme	immunogenic	985:995	arg1	Site					1005:1008	the main immunogenic epitope Site Ø	976:1010	the main immunogenic epitope Site Ø	976:1010	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	1	11	attach	linked	256:261	arg1	efficacy					286:293	efficacy	286:293	efficacy	286:293	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	1	11	attach	linked	256:261	arg1	quality					274:280	product quality	266:280	product quality	266:280	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	1	11	attach	linked	256:261	arg2	product					229:235	a protein-based therapeutic or vaccine product	190:235	a protein-based therapeutic or vaccine product	190:235	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	3	12	theme	complete	534:541	arg1	glycan					552:557	a complete N-linked glycan	532:557	a complete N-linked glycan	532:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	2	13	theme	product	389:395	arg1	understanding					368:380	thorough understanding	359:380	thorough understanding of the product	359:395	Elucidating protein degradation pathways is required to obtain thorough understanding of the product and ensure degradation products are properly monitored.					
30152690	0	14	theme	Stabilized	99:108	arg1	Form					110:113	a Stabilized Form	97:113	a Stabilized Form of RSV Prefusion F Protein	97:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	4	15	theme	glycine	849:855	arg1	residue					857:863	a glycine residue	847:863	a glycine residue immediately following the site of glycosylation	847:911	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	3	16	theme	N-linked	543:550	arg1	glycan					552:557	a complete N-linked glycan	532:557	a complete N-linked glycan	532:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	7	17	theme	degradation	1361:1371	arg1	type					1353:1356	this new type	1344:1356	this new type	1344:1356	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	18	theme	important	1204:1212	arg1	glycosylation					1184:1196	glycosylation	1184:1196	glycosylation	1184:1196	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	18	theme	important	1204:1212	arg1	attribute					1214:1222	an important attribute	1201:1222	an important attribute for many recombinant therapeutic proteins or vaccine antigens	1201:1284	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	19	theme	vaccine	1269:1275	arg1	antigens					1277:1284	vaccine antigens	1269:1284	vaccine antigens	1269:1284	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	20	theme	new	1349:1351	arg1	type					1353:1356	this new type	1344:1356	this new type	1344:1356	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	0	21	theme	Non-Enzymatic	0:12	arg1	Shedding					39:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	0	22	dep	Pathway	77:83	arg1	Shedding					39:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	1	23	theme	critical	167:174	arg1	attributes					176:185	the critical attributes	163:185	the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy	163:293	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	0	24	theme	Glycan	32:37	arg1	Shedding					39:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	0	25	theme	RSV	118:120	arg1	Protein					134:140	RSV Prefusion F Protein	118:140	RSV Prefusion F Protein	118:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	3	26	theme	stress	565:570	arg1	condition					572:580	stress condition	565:580	stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	565:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	5	27	theme	antibody	1033:1040	arg1	D25					1042:1044	the neutralizing antibody D25	1016:1044	the neutralizing antibody D25	1016:1044	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	1	28	theme	attributes	176:185	arg1	one					156:158	one	156:158	one	156:158	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	1	28	theme	attributes	176:185	arg1	attributes					176:185	the critical attributes	163:185	the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy	163:293	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	0	29	theme	Site-Specific	18:30	arg1	Shedding					39:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding	0:46	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	1	30	theme	product	266:272	arg1	quality					274:280	product quality	266:280	product quality	266:280	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	5	31	theme	epitope	997:1003	arg1	Site					1005:1008	the main immunogenic epitope Site Ø	976:1010	the main immunogenic epitope Site Ø	976:1010	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	7	32	from	study	1309:1313	arg1	finding					1291:1297	the finding	1287:1297	the finding from this study	1287:1313	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	33	from	impact	1411:1416	arg1	safety					1433:1438	safety	1433:1438	safety	1433:1438	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	7	33	from	impact	1411:1416	arg1	efficacy					1421:1428	efficacy	1421:1428	efficacy	1421:1428	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	3	34	theme	unique	467:472	arg1	degradation					482:492	a unique protein degradation	465:492	a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	465:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	5	35	theme	main	980:983	arg1	Site					1005:1008	the main immunogenic epitope Site Ø	976:1010	the main immunogenic epitope Site Ø	976:1010	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	0	36	theme	F	132:132	arg1	Protein					134:140	RSV Prefusion F Protein	118:140	RSV Prefusion F Protein	118:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	7	37	theme	many	1228:1231	arg1	proteins					1257:1264	many recombinant therapeutic proteins	1228:1264	many recombinant therapeutic proteins	1228:1264	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	3	38	theme	syncytial	613:621	arg1	RSV					630:632	RSV	630:632	RSV	630:632	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	38	theme	syncytial	613:621	arg1	virus					623:627	respiratory syncytial virus	601:627	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	4	39	gly	glycosylation	899:911	arg2	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	4	39	gly	glycosylation	899:911	arg2	site					891:894	the site	887:894	the site of glycosylation	887:911	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	6	40	theme	Further	1047:1053	arg1	study					1055:1059	Further study	1047:1059	Further study	1047:1059	Further study indicated that the glycan shedding followed a similar but different mechanism than that of conventional deamidation.					
30152690	0	41	theme	Prefusion	122:130	arg1	Protein					134:140	RSV Prefusion F Protein	118:140	RSV Prefusion F Protein	118:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	3	42	theme	F	645:645	arg1	RSVPreF3					656:663	RSVPreF3	656:663	RSVPreF3	656:663	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	42	theme	F	645:645	arg1	protein					647:653	an engineered respiratory syncytial virus (RSV) prefusion F protein	587:653	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	43	theme	virus	623:627	arg1	RSVPreF3					656:663	RSVPreF3	656:663	RSVPreF3	656:663	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	43	theme	virus	623:627	arg1	protein					647:653	an engineered respiratory syncytial virus (RSV) prefusion F protein	587:653	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	1	44	theme	protein-based	192:204	arg1	product					229:235	a protein-based therapeutic or vaccine product	190:235	a protein-based therapeutic or vaccine product	190:235	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	0	45	theme	Novel	51:55	arg1	Pathway					77:83	A Novel Protein Degradation Pathway	49:83	A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein	49:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	3	46	theme	engineered	590:599	arg1	RSVPreF3					656:663	RSVPreF3	656:663	RSVPreF3	656:663	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	46	theme	engineered	590:599	arg1	protein					647:653	an engineered respiratory syncytial virus (RSV) prefusion F protein	587:653	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	6	47	theme	conventional	1152:1163	arg1	deamidation					1165:1175	conventional deamidation	1152:1175	conventional deamidation	1152:1175	Further study indicated that the glycan shedding followed a similar but different mechanism than that of conventional deamidation.					
30152690	6	48	theme	glycan	1080:1085	arg1	shedding					1087:1094	the glycan shedding	1076:1094	the glycan shedding	1076:1094	Further study indicated that the glycan shedding followed a similar but different mechanism than that of conventional deamidation.					
30152690	0	49	theme	Protein	134:140	arg1	Form					110:113	a Stabilized Form	97:113	a Stabilized Form of RSV Prefusion F Protein	97:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	3	50	theme	respiratory	601:611	arg1	RSV					630:632	RSV	630:632	RSV	630:632	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	50	theme	respiratory	601:611	arg1	virus					623:627	respiratory syncytial virus	601:627	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	7	51	theme	therapeutic	1245:1255	arg1	proteins					1257:1264	many recombinant therapeutic proteins	1228:1264	many recombinant therapeutic proteins	1228:1264	Since glycosylation is an important attribute for many recombinant therapeutic proteins or vaccine antigens, the finding from this study suggests the need to monitor this new type of degradation, especially when glycosylation has an impact on efficacy or safety.					
30152690	3	52	theme	nonenzyme	504:512	arg1	loss					524:527	nonenzyme catalyzed loss	504:527	nonenzyme catalyzed loss of a complete N-linked glycan	504:557	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	5	53	theme	neutralizing	1020:1031	arg1	antibody					1033:1040	the neutralizing antibody	1016:1040	the neutralizing antibody D25	1016:1044	The glycan loss did not negatively affect the binding between the main immunogenic epitope Site Ø and the neutralizing antibody D25.					
30152690	2	54	theme	degradation	316:326	arg1	pathways					328:335	protein degradation pathways	308:335	protein degradation pathways	308:335	Elucidating protein degradation pathways is required to obtain thorough understanding of the product and ensure degradation products are properly monitored.					
30152690	4	55	theme	glycan	765:770	arg1	shedding					772:779	the glycan shedding	761:779	the glycan shedding	761:779	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	1	56	theme	therapeutic	206:216	arg1	product					229:235	a protein-based therapeutic or vaccine product	190:235	a protein-based therapeutic or vaccine product	190:235	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	3	57	theme	prefusion	635:643	arg1	RSVPreF3					656:663	RSVPreF3	656:663	RSVPreF3	656:663	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	3	57	theme	prefusion	635:643	arg1	protein					647:653	an engineered respiratory syncytial virus (RSV) prefusion F protein	587:653	an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	587:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	2	58	theme	protein	308:314	arg1	pathways					328:335	protein degradation pathways	308:335	protein degradation pathways	308:335	Elucidating protein degradation pathways is required to obtain thorough understanding of the product and ensure degradation products are properly monitored.					
30152690	1	59	theme	or	218:219	arg1	product					229:235	a protein-based therapeutic or vaccine product	190:235	a protein-based therapeutic or vaccine product	190:235	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	0	60	theme	Degradation	65:75	arg1	Pathway					77:83	A Novel Protein Degradation Pathway	49:83	A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein	49:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	6	61	theme	different	1119:1127	arg1	mechanism					1129:1137	a similar but different mechanism	1105:1137	a similar but different mechanism than that of conventional deamidation	1105:1175	Further study indicated that the glycan shedding followed a similar but different mechanism than that of conventional deamidation.					
30152690	2	62	theme	thorough	359:366	arg1	understanding					368:380	thorough understanding	359:380	thorough understanding of the product	359:395	Elucidating protein degradation pathways is required to obtain thorough understanding of the product and ensure degradation products are properly monitored.					
30152690	1	63	theme	vaccine	221:227	arg1	product					229:235	a protein-based therapeutic or vaccine product	190:235	a protein-based therapeutic or vaccine product	190:235	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
30152690	0	64	theme	Protein	57:63	arg1	Pathway					77:83	A Novel Protein Degradation Pathway	49:83	A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein	49:140	Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein.					
30152690	4	65	theme	structural	813:822	arg1	elements					824:831	structural elements	813:831	structural elements	813:831	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	3	66	from	protein	647:653	arg1	condition					572:580	stress condition	565:580	stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3)	565:664	We observed a unique protein degradation involving nonenzyme catalyzed loss of a complete N-linked glycan under stress condition from an engineered respiratory syncytial virus (RSV) prefusion F protein (RSVPreF3).					
30152690	4	67	theme	mass	692:695	arg1	spectrometry					697:708	mass spectrometry	692:708	mass spectrometry	692:708	Investigations involving mass spectrometry, molecular modeling, and mutagenesis revealed that the glycan shedding was site-specific, dependent on structural elements, and required a glycine residue immediately following the site of glycosylation.					
30152690	1	68	theme	product	229:235	arg1	attributes					176:185	the critical attributes	163:185	the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy	163:293	Stability is one of the critical attributes of a protein-based therapeutic or vaccine product, which is directly linked to product quality and efficacy.					
29740059	7	0	theme	glycoproteins	1428:1440	arg1	efficacy					1398:1405	the efficacy	1394:1405	the efficacy of other therapeutic glycoproteins	1394:1440	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	1	1	gly	glycoproteins	193:205	arg1	glycoproteins					193:205	recombinant therapeutic glycoproteins	169:205	recombinant therapeutic glycoproteins	169:205	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	3	2	theme	poly-N-acetyllactosamine	537:560	arg1	biosynthesis					576:587	poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	537:587	poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	537:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	4	3	theme	rhEPO	624:628	arg1	sialylation					630:640	rhEPO sialylation	624:640	rhEPO sialylation	624:640	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	3	4	gly	sialylation	445:455	arg1	erythropoietin					478:491	recombinant human erythropoietin	460:491	recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	460:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	3	4	gly	sialylation	445:455	arg1	rhEPO					494:498	rhEPO	494:498	rhEPO	494:498	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	5	5	from	increase	969:976	arg1	sialylation					987:997	rhEPO sialylation	981:997	rhEPO sialylation in metabolically engineered CHO cells	981:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	1	6	theme	recombinant	169:179	arg1	glycoproteins					193:205	recombinant therapeutic glycoproteins	169:205	recombinant therapeutic glycoproteins	169:205	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	5	7	theme	significant	957:967	arg1	increase					969:976	the most significant increase	948:976	the most significant increase in rhEPO sialylation in metabolically engineered CHO cells	948:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	4	8	theme	monophosphate-N-acetylneuraminic	708:739	arg1	CMP-Neu5Ac					747:756	CMP-Neu5Ac	747:756	CMP-Neu5Ac	747:756	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	8	theme	monophosphate-N-acetylneuraminic	708:739	arg1	acid					741:744	intracellular cytidine monophosphate-N-acetylneuraminic acid	685:744	intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac)	685:757	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	8	theme	monophosphate-N-acetylneuraminic	708:739	arg1	factor					780:785	a limiting factor	769:785	a limiting factor for sialylation	769:801	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	2	9	theme	sites	369:373	arg1	number					338:343	the number	334:343	the number of potential attachment sites for sialic acid	334:389	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	5	10	dep	approach	849:856	arg1	releasing					898:906	releasing	898:906	releasing CMP-Neu5Ac feedback inhibition	898:937	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	5	10	dep	approach	849:856	arg1	inhibiting					858:867	inhibiting	858:867	inhibiting poly-LacNAc biosynthesis	858:892	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	7	11	from	useful	1358:1363	arg1	efforts					1375:1381	future efforts	1368:1381	future efforts	1368:1381	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	5	12	theme	feedback	919:926	arg1	inhibition					928:937	CMP-Neu5Ac feedback inhibition	908:937	CMP-Neu5Ac feedback inhibition	908:937	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	2	13	gly	glycoproteins	310:322	arg1	glycoproteins					310:322	glycoproteins	310:322	glycoproteins	310:322	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	6	14	theme	structures	1239:1248	arg1	reduction					1203:1211	a reduction	1201:1211	a reduction of di-sialylated N-glycan structures	1201:1248	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	7	15	theme	new	1278:1280	arg1	approach					1282:1289	our new approach	1274:1289	our new approach for glycosylation engineering	1274:1319	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	7	16	theme	other	1410:1414	arg1	glycoproteins					1428:1440	other therapeutic glycoproteins	1410:1440	other therapeutic glycoproteins	1410:1440	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	6	17	theme	sialylated	1155:1164	arg1	structures					1175:1184	sialylated N-glycan structures	1155:1184	sialylated N-glycan structures	1155:1184	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	1	18	theme	therapeutic	181:191	arg1	glycoproteins					193:205	recombinant therapeutic glycoproteins	169:205	recombinant therapeutic glycoproteins	169:205	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	0	19	theme	recombinant	115:125	arg1	EPO					127:129	recombinant EPO	115:129	recombinant EPO produced in CHO cells	115:151	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	6	20	theme	di-sialylated	1216:1228	arg1	structures					1239:1248	di-sialylated N-glycan structures	1216:1248	di-sialylated N-glycan structures	1216:1248	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	5	21	theme	engineered	1016:1025	arg1	cells					1031:1035	metabolically engineered CHO cells	1002:1035	metabolically engineered CHO cells	1002:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	1	22	theme	glycoproteins	193:205	arg1	Sialylation					154:164	Sialylation	154:164	Sialylation of recombinant therapeutic glycoproteins	154:205	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	4	23	from	increase	612:619	arg1	sialylation					630:640	rhEPO sialylation	624:640	rhEPO sialylation	624:640	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	7	24	gly	glycoproteins	1428:1440	arg1	glycoproteins					1428:1440	other therapeutic glycoproteins	1410:1440	other therapeutic glycoproteins	1410:1440	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	7	25	from	efforts	1375:1381	arg1	useful					1358:1363	useful	1358:1363	useful	1358:1363	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	0	26	gly	sialylation	100:110	arg1	EPO					127:129	recombinant EPO	115:129	recombinant EPO produced in CHO cells	115:151	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	3	27	theme	recombinant	460:470	arg1	erythropoietin					478:491	recombinant human erythropoietin	460:491	recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	460:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	3	27	theme	recombinant	460:470	arg1	rhEPO					494:498	rhEPO	494:498	rhEPO	494:498	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	6	28	theme	detailed	1053:1060	arg1	analysis					1062:1069	a detailed analysis	1051:1069	a detailed analysis of the resulting N-glycan structures using LC/MS	1051:1118	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	6	29	gly	di-sialylated	1216:1228	arg1	structures					1239:1248	di-sialylated N-glycan structures	1216:1248	di-sialylated N-glycan structures	1216:1248	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	2	30	theme	attachment	358:367	arg1	sites					369:373	potential attachment sites	348:373	potential attachment sites for sialic acid	348:389	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	6	31	theme	resulting	1078:1086	arg1	structures					1097:1106	the resulting N-glycan structures	1074:1106	the resulting N-glycan structures using LC/MS	1074:1118	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	3	32	theme	human	472:476	arg1	erythropoietin					478:491	recombinant human erythropoietin	460:491	recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	460:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	3	32	theme	human	472:476	arg1	rhEPO					494:498	rhEPO	494:498	rhEPO	494:498	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	0	33	theme	biosynthesis	26:37	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition	0:84	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	6	34	theme	N-glycan	1166:1173	arg1	structures					1175:1184	sialylated N-glycan structures	1155:1184	sialylated N-glycan structures	1155:1184	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	5	35	gly	sialylation	987:997	arg1	cells					1031:1035	metabolically engineered CHO cells	1002:1035	metabolically engineered CHO cells	1002:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	5	36	from	sialylation	987:997	arg1	cells					1031:1035	metabolically engineered CHO cells	1002:1035	metabolically engineered CHO cells	1002:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	2	37	theme	potential	348:356	arg1	sites					369:373	potential attachment sites	348:373	potential attachment sites for sialic acid	348:389	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	1	38	theme	pharmacokinetic	223:237	arg1	properties					239:248	their pharmacokinetic properties	217:248	their pharmacokinetic properties	217:248	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	0	39	theme	poly-LacNAc	14:24	arg1	biosynthesis					26:37	poly-LacNAc biosynthesis	14:37	poly-LacNAc biosynthesis	14:37	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	5	40	theme	CHO	1027:1029	arg1	cells					1031:1035	metabolically engineered CHO cells	1002:1035	metabolically engineered CHO cells	1002:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	7	41	theme	therapeutic	1416:1426	arg1	glycoproteins					1428:1440	other therapeutic glycoproteins	1410:1440	other therapeutic glycoproteins	1410:1440	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	4	42	theme	feedback	662:669	arg1	inhibition					671:680	the feedback inhibition	658:680	the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation,	658:802	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	6	43	theme	increased	1129:1137	arg1	tri-					1139:1142	increased tri-	1129:1142	increased tri-	1129:1142	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	0	44	theme	EPO	127:129	arg1	sialylation					100:110	the sialylation	96:110	the sialylation of recombinant EPO produced in CHO cells	96:151	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	2	45	from	branching	297:305	arg1	glycoproteins					310:322	glycoproteins	310:322	glycoproteins	310:322	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	4	46	theme	intracellular	685:697	arg1	CMP-Neu5Ac					747:756	CMP-Neu5Ac	747:756	CMP-Neu5Ac	747:756	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	46	theme	intracellular	685:697	arg1	acid					741:744	intracellular cytidine monophosphate-N-acetylneuraminic acid	685:744	intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac)	685:757	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	46	theme	intracellular	685:697	arg1	factor					780:785	a limiting factor	769:785	a limiting factor for sialylation	769:801	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	3	47	theme	erythropoietin	478:491	arg1	sialylation					445:455	the sialylation	441:455	the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	441:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	0	48	theme	CHO	143:145	arg1	cells					147:151	CHO cells	143:151	CHO cells	143:151	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	5	49	theme	CMP-Neu5Ac	908:917	arg1	inhibition					928:937	CMP-Neu5Ac feedback inhibition	908:937	CMP-Neu5Ac feedback inhibition	908:937	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	0	50	theme	CMP-Neu5Ac	55:64	arg1	inhibition					75:84	CMP-Neu5Ac feedback inhibition	55:84	CMP-Neu5Ac feedback inhibition	55:84	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	1	51	dep	in	269:270	arg1	vivo					272:275	vivo	272:275	vivo	272:275	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	5	52	theme	rhEPO	981:985	arg1	sialylation					987:997	rhEPO sialylation	981:997	rhEPO sialylation in metabolically engineered CHO cells	981:1035	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	3	53	theme	CHO	513:515	arg1	cells					517:521	CHO cells	513:521	CHO cells	513:521	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	6	54	theme	N-glycan	1230:1237	arg1	structures					1239:1248	di-sialylated N-glycan structures	1216:1248	di-sialylated N-glycan structures	1216:1248	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	0	55	with	Inhibition	0:9	arg1	release					44:50	release	44:50	release of CMP-Neu5Ac feedback inhibition	44:84	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	2	56	theme	sialic	379:384	arg1	acid					386:389	sialic acid	379:389	sialic acid	379:389	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	6	57	gly	sialylated	1155:1164	arg1	structures					1175:1184	sialylated N-glycan structures	1155:1184	sialylated N-glycan structures	1155:1184	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	5	58	theme	poly-LacNAc	869:879	arg1	biosynthesis					881:892	poly-LacNAc biosynthesis	869:892	poly-LacNAc biosynthesis	869:892	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	4	59	theme	limiting	771:778	arg1	acid					741:744	intracellular cytidine monophosphate-N-acetylneuraminic acid	685:744	intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac)	685:757	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	59	theme	limiting	771:778	arg1	factor					780:785	a limiting factor	769:785	a limiting factor for sialylation	769:801	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	1	60	theme	in	269:270	arg1	half-life					277:285	their in vivo half-life	263:285	their in vivo half-life	263:285	Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life.					
29740059	0	61	theme	inhibition	75:84	arg1	release					44:50	release	44:50	release of CMP-Neu5Ac feedback inhibition	44:84	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	2	62	theme	N-glycan	288:295	arg1	branching					297:305	N-glycan branching	288:305	N-glycan branching on glycoproteins	288:322	N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid.					
29740059	6	63	dep	tri-	1139:1142	arg1	structures					1175:1184	sialylated N-glycan structures	1155:1184	sialylated N-glycan structures	1155:1184	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	4	64	theme	cytidine	699:706	arg1	CMP-Neu5Ac					747:756	CMP-Neu5Ac	747:756	CMP-Neu5Ac	747:756	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	64	theme	cytidine	699:706	arg1	acid					741:744	intracellular cytidine monophosphate-N-acetylneuraminic acid	685:744	intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac)	685:757	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	4	64	theme	cytidine	699:706	arg1	factor					780:785	a limiting factor	769:785	a limiting factor for sialylation	769:801	We did not observe an increase in rhEPO sialylation, however, until the feedback inhibition by intracellular cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), which is a limiting factor for sialylation, was released.					
29740059	6	65	theme	structures	1097:1106	arg1	analysis					1062:1069	a detailed analysis	1051:1069	a detailed analysis of the resulting N-glycan structures using LC/MS	1051:1118	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29740059	0	66	theme	feedback	66:73	arg1	inhibition					75:84	CMP-Neu5Ac feedback inhibition	55:84	CMP-Neu5Ac feedback inhibition	55:84	Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells.					
29740059	7	67	theme	glycosylation	1295:1307	arg1	engineering					1309:1319	glycosylation engineering	1295:1319	glycosylation engineering	1295:1319	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	3	68	theme	new	413:415	arg1	approach					417:424	a new approach	411:424	a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis	411:587	Here, we introduce a new approach for increasing the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells by modulating poly-N-acetyllactosamine (poly-LacNAc) biosynthesis.					
29740059	7	69	theme	future	1368:1373	arg1	efforts					1375:1381	future efforts	1368:1381	future efforts	1368:1381	These results validate our new approach for glycosylation engineering, and we expect this approach will be useful in future efforts to enhance the efficacy of other therapeutic glycoproteins.					
29740059	5	70	theme	combined	840:847	arg1	approach					849:856	a combined approach	838:856	a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition	838:937	Thus, we found that a combined approach inhibiting poly-LacNAc biosynthesis and releasing CMP-Neu5Ac feedback inhibition produces the most significant increase in rhEPO sialylation in metabolically engineered CHO cells.					
29740059	6	71	theme	N-glycan	1088:1095	arg1	structures					1097:1106	the resulting N-glycan structures	1074:1106	the resulting N-glycan structures using LC/MS	1074:1118	Furthermore, a detailed analysis of the resulting N-glycan structures using LC/MS revealed increased tri- and tetra- sialylated N-glycan structures accompanied by a reduction of di-sialylated N-glycan structures.					
29881382	4	0	theme	N-glycan	946:953	arg1	processing					955:964	proper N-glycan processing	939:964	proper N-glycan processing	939:964	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	10	1	theme	increased	1766:1774	arg1	neutralization					1776:1789	increased neutralization	1766:1789	increased neutralization of tier 1 viruses	1766:1807	In addition, increased neutralization of tier 1 viruses was detected.					
29881382	9	2	theme	CHO-M	1725:1729	arg1	cell					1731:1734	CHO-M cell	1725:1734	CHO-M cell	1725:1734	These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers.					
29881382	3	3	theme	BG505	587:591	arg1	trimers					597:603	BG505 NFL trimers	587:603	BG505 NFL trimers	587:603	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	9	4	theme	derived	1736:1742	arg1	trimers					1744:1750	CHO-M cell derived trimers	1725:1750	CHO-M cell derived trimers	1725:1750	These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers.					
29881382	4	5	theme	BG505	660:664	arg1	trimers					670:676	BG505 NFL trimers	660:676	BG505 NFL trimers purified by single-step lectin-affinity chromatography	660:731	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	1	6	dep	native-like	222:232	arg1	well-folded					235:245	well-folded	235:245	well-folded	235:245	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	4	7	theme	native-like	745:755	arg1	structure					764:772	a native-like closed structure	743:772	a native-like closed structure	743:772	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	1	8	theme	linked	128:133	arg1	Env					170:172	Env	170:172	Env	170:172	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	1	8	theme	linked	128:133	arg1	glycoprotein					156:167	flexibly linked (NFL) HIV-1 envelope glycoprotein	119:167	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	8	9	theme	BG505	1500:1504	arg1	trimers					1512:1518	BG505 SOSIP trimers	1500:1518	BG505 SOSIP trimers	1500:1518	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	10	10	theme	tier	1794:1797	arg1	viruses					1801:1807	tier 1 viruses	1794:1807	tier 1 viruses	1794:1807	In addition, increased neutralization of tier 1 viruses was detected.					
29881382	4	11	theme	lectin-affinity	702:716	arg1	chromatography					718:731	single-step lectin-affinity chromatography	690:731	single-step lectin-affinity chromatography	690:731	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	10	12	located	detected	1813:1820	arg1	addition					1756:1763	addition	1756:1763	addition	1756:1763	In addition, increased neutralization of tier 1 viruses was detected.					
29881382	10	12	located	detected	1813:1820	arg2	neutralization					1776:1789	increased neutralization	1766:1789	increased neutralization of tier 1 viruses	1766:1807	In addition, increased neutralization of tier 1 viruses was detected.					
29881382	3	13	theme	homogeneity	624:634	arg1	level					615:619	a high level	608:619	a high level of homogeneity	608:634	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	3	13	theme	homogeneity	624:634	arg1	yields					640:645	yields	640:645	yields of ~1.8 g/l	640:657	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	1	14	theme	HIV-1	141:145	arg1	Env					170:172	Env	170:172	Env	170:172	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	1	14	theme	HIV-1	141:145	arg1	glycoprotein					156:167	flexibly linked (NFL) HIV-1 envelope glycoprotein	119:167	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	0	15	theme	Tier	80:83	arg1	Antibodies					100:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	1	16	theme	glycoprotein	156:167	arg1	trimers					175:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	0	17	theme	Neutralizing	87:98	arg1	Antibodies					100:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	0	18	from	Lines	49:53	arg1	Trimers					27:33	Cleavage-Independent HIV-1 Trimers	0:33	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines	0:53	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	2	19	theme	precursor-cleaving	426:443	arg1	furin					455:459	furin	455:459	furin that is required by the cleavage-dependent SOSIP trimers	455:516	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	2	19	theme	precursor-cleaving	426:443	arg1	protease					445:452	the precursor-cleaving protease	422:452	the precursor-cleaving protease	422:452	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	0	20	theme	Autologous	69:78	arg1	Antibodies					100:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	4	21	theme	efficient	775:783	arg1	recognition					785:795	efficient recognition	775:795	efficient recognition by trimer-preferring bNAbs	775:822	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	0	22	theme	Cleavage-Independent	0:19	arg1	Trimers					27:33	Cleavage-Independent HIV-1 Trimers	0:33	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines	0:53	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	1	23	theme	Native	112:117	arg1	trimers					175:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	7	24	theme	virus	1356:1360	arg1	neutralization					1362:1375	low-to-no tier 1 virus neutralization	1339:1375	low-to-no tier 1 virus neutralization	1339:1375	The BG505 NFL trimers that did not expose V3 in vitro, elicited low-to-no tier 1 virus neutralization in vivo, indicating that they remained intact during the immunization process, not exposing V3.					
29881382	0	25	theme	Robust	62:67	arg1	Antibodies					100:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Robust Autologous Tier 2 Neutralizing Antibodies	62:109	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	0	26	theme	CHO	40:42	arg1	Lines					49:53	CHO Cell Lines	40:53	CHO Cell Lines	40:53	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	7	27	theme	tier	1349:1352	arg1	neutralization					1362:1375	low-to-no tier 1 virus neutralization	1339:1375	low-to-no tier 1 virus neutralization	1339:1375	The BG505 NFL trimers that did not expose V3 in vitro, elicited low-to-no tier 1 virus neutralization in vivo, indicating that they remained intact during the immunization process, not exposing V3.					
29881382	2	28	theme	SOSIP	504:508	arg1	trimers					510:516	the cleavage-dependent SOSIP trimers	481:516	the cleavage-dependent SOSIP trimers	481:516	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	8	29	theme	BG505	1605:1609	arg1	titers					1642:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	3	30	theme	~1.8 g/l	650:657	arg1	level					615:619	a high level	608:619	a high level of homogeneity	608:634	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	3	30	theme	~1.8 g/l	650:657	arg1	yields					640:645	yields	640:645	yields of ~1.8 g/l	640:657	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	4	31	theme	V3-directed	890:900	arg1	antibodies					902:911	non-neutralizing CD4 binding site-directed and V3-directed antibodies	843:911	antibodies	902:911	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	2	32	theme	Env	378:380	arg1	production					364:373	large-scale production	352:373	large-scale production of Env	352:380	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	3	33	theme	cell	562:565	arg1	line					567:570	a CHO-M cell line	554:570	a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l	554:657	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	8	34	theme	neutralizing	1629:1640	arg1	titers					1642:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	5	35	theme	autologous	1098:1107	arg1	antibodies					1129:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	6	36	theme	present	1197:1203	arg1	"					1185:1185	the N-glycan "hole"	1167:1185	the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289	1167:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	3	37	theme	line	567:570	arg1	development					539:549	the development	535:549	the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l	535:657	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	4	38	theme	proper	939:944	arg1	processing					955:964	proper N-glycan processing	939:964	proper N-glycan processing	939:964	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	3	39	theme	CHO-M	556:560	arg1	line					567:570	a CHO-M cell line	554:570	a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l	554:657	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	4	40	theme	NFL	666:668	arg1	trimers					670:676	BG505 NFL trimers	660:676	BG505 NFL trimers purified by single-step lectin-affinity chromatography	660:731	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	3	41	theme	NFL	593:595	arg1	trimers					597:603	BG505 NFL trimers	587:603	BG505 NFL trimers	587:603	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	4	42	theme	long-term	914:922	arg1	stability					924:932	long-term stability	914:932	long-term stability	914:932	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	2	43	theme	NFL	328:330	arg1	platform					332:339	The NFL platform	324:339	The NFL platform	324:339	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	3	44	theme	high	610:613	arg1	level					615:619	a high level	608:619	a high level of homogeneity	608:634	Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l.					
29881382	7	45	theme	immunization	1434:1445	arg1	process					1447:1453	the immunization process	1430:1453	the immunization process	1430:1453	The BG505 NFL trimers that did not expose V3 in vitro, elicited low-to-no tier 1 virus neutralization in vivo, indicating that they remained intact during the immunization process, not exposing V3.					
29881382	11	46	theme	responses	1964:1972	arg1	elicitation					1918:1928	the elicitation	1914:1928	the elicitation of tier 2 and tier 1 neutralizing responses	1914:1972	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	8	47	theme	SOSIP	1506:1510	arg1	trimers					1512:1518	BG505 SOSIP trimers	1500:1518	BG505 SOSIP trimers	1500:1518	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	1	48	gly	glycoprotein	156:167	arg1	Env					170:172	Env	170:172	Env	170:172	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	1	48	gly	glycoprotein	156:167	arg1	glycoprotein					156:167	flexibly linked (NFL) HIV-1 envelope glycoprotein	119:167	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	10	49	theme	viruses	1801:1807	arg1	neutralization					1776:1789	increased neutralization	1766:1789	increased neutralization of tier 1 viruses	1766:1807	In addition, increased neutralization of tier 1 viruses was detected.					
29881382	4	50	theme	closed	757:762	arg1	structure					764:772	a native-like closed structure	743:772	a native-like closed structure	743:772	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	9	51	dep	derived	1736:1742	arg1	cell					1731:1734	CHO-M cell	1725:1734	CHO-M cell	1725:1734	These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers.					
29881382	8	52	theme	293F	1535:1538	arg1	cells					1540:1544	293F cells	1535:1544	293F cells	1535:1544	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	4	53	theme	single-step	690:700	arg1	chromatography					718:731	single-step lectin-affinity chromatography	690:731	single-step lectin-affinity chromatography	690:731	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	1	54	theme	envelope	147:154	arg1	Env					170:172	Env	170:172	Env	170:172	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	1	54	theme	envelope	147:154	arg1	glycoprotein					156:167	flexibly linked (NFL) HIV-1 envelope glycoprotein	119:167	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	5	55	from	negative-selection	977:994	arg1	adjuvant					1023:1030	adjuvant	1023:1030	adjuvant	1023:1030	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	4	56	theme	trimer-preferring	800:816	arg1	bNAbs					818:822	trimer-preferring bNAbs	800:822	trimer-preferring bNAbs	800:822	BG505 NFL trimers purified by single-step lectin-affinity chromatography displayed a native-like closed structure, efficient recognition by trimer-preferring bNAbs, no recognition by non-neutralizing CD4 binding site-directed and V3-directed antibodies, long-term stability, and proper N-glycan processing.					
29881382	2	57	theme	large-scale	352:362	arg1	production					364:373	large-scale production	352:373	large-scale production of Env	352:380	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	6	58	theme	hole	1181:1184	arg1	"					1185:1185	the N-glycan "hole"	1167:1185	the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289	1167:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	1	59	dep	linked	128:133	arg1	NFL					136:138	NFL	136:138	NFL	136:138	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	0	60	theme	HIV-1	21:25	arg1	Trimers					27:33	Cleavage-Independent HIV-1 Trimers	0:33	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines	0:53	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	6	61	theme	N-glycan	1171:1178	arg1	"					1185:1185	the N-glycan "hole"	1167:1185	the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289	1167:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	1	62	theme	neutralizing	297:308	arg1	determinants					310:321	broadly neutralizing determinants	289:321	broadly neutralizing determinants	289:321	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	5	63	from	formulation	997:1007	arg1	adjuvant					1023:1030	adjuvant	1023:1030	adjuvant	1023:1030	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	7	64	dep	expose	1310:1315	arg1	elicited					1330:1337	elicited	1330:1337	elicited low-to-no tier 1 virus neutralization in vivo	1330:1383	The BG505 NFL trimers that did not expose V3 in vitro, elicited low-to-no tier 1 virus neutralization in vivo, indicating that they remained intact during the immunization process, not exposing V3.					
29881382	8	65	theme	equivalent	1594:1603	arg1	titers					1642:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	6	66	attach	present	1197:1203	arg2	"					1185:1185	the N-glycan "hole"	1167:1185	the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289	1167:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	6	66	attach	present	1197:1203	arg1	Env					1218:1220	the BG505 Env	1208:1220	the BG505 Env proximal to residues at positions 230, 241, and 289	1208:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	6	67	from	positions	1246:1254	arg1	residues					1234:1241	residues	1234:1241	residues at positions 230, 241, and 289	1234:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	11	68	theme	neutralizing	1951:1962	arg1	responses					1964:1972	tier 2 and tier 1 neutralizing responses	1933:1972	responses	1964:1972	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	0	69	theme	Cell	44:47	arg1	Lines					49:53	CHO Cell Lines	40:53	CHO Cell Lines	40:53	Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.					
29881382	5	70	theme	tier	1109:1112	arg1	antibodies					1129:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	7	71	theme	low-to-no	1339:1347	arg1	neutralization					1362:1375	low-to-no tier 1 virus neutralization	1339:1375	low-to-no tier 1 virus neutralization	1339:1375	The BG505 NFL trimers that did not expose V3 in vitro, elicited low-to-no tier 1 virus neutralization in vivo, indicating that they remained intact during the immunization process, not exposing V3.					
29881382	11	72	theme	tier	1933:1936	arg1	elicitation					1918:1928	the elicitation	1914:1928	the elicitation of tier 2 and tier 1 neutralizing responses	1914:1972	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	8	73	theme	tier	1611:1614	arg1	titers					1642:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	11	74	theme	tier	1944:1947	arg1	responses					1964:1972	tier 2 and tier 1 neutralizing responses	1933:1972	responses	1964:1972	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	5	75	theme	neutralizing	1116:1127	arg1	antibodies					1129:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	6	76	theme	proximal	1222:1229	arg1	Env					1218:1220	the BG505 Env	1208:1220	the BG505 Env proximal to residues at positions 230, 241, and 289	1208:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	2	77	theme	cleavage-dependent	485:502	arg1	trimers					510:516	the cleavage-dependent SOSIP trimers	481:516	the cleavage-dependent SOSIP trimers	481:516	The NFL platform simplifies large-scale production of Env by eliminating the need to co-transfect the precursor-cleaving protease, furin that is required by the cleavage-dependent SOSIP trimers.					
29881382	8	78	theme	autologous	1618:1627	arg1	titers					1642:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	equivalent BG505 tier 2 autologous neutralizing titers	1594:1647	In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers.					
29881382	11	79	theme	cell-type	1882:1890	arg1	expression					1892:1901	both adjuvant and cell-type expression	1864:1901	both adjuvant and cell-type expression	1864:1901	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	5	80	from	inoculation	1036:1046	arg1	adjuvant					1023:1030	adjuvant	1023:1030	adjuvant	1023:1030	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	6	81	theme	BG505	1212:1216	arg1	Env					1218:1220	the BG505 Env	1208:1220	the BG505 Env proximal to residues at positions 230, 241, and 289	1208:1272	These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289.					
29881382	1	82	link	linked	128:133	arg1	Env					170:172	Env	170:172	Env	170:172	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	1	82	link	linked	128:133	arg1	glycoprotein					156:167	flexibly linked (NFL) HIV-1 envelope glycoprotein	119:167	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers	112:181	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	11	83	theme	adjuvant	1869:1876	arg1	expression					1892:1901	both adjuvant and cell-type expression	1864:1901	both adjuvant and cell-type expression	1864:1901	Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo.					
29881382	1	84	theme	native-like	222:232	arg1	conformation					247:258	a native-like, well-folded conformation	220:258	a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants	220:321	Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants.					
29881382	5	85	theme	potent	1091:1096	arg1	antibodies					1129:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	potent autologous tier 2 neutralizing antibodies	1091:1138	Following negative-selection, formulation in ISCOMATRIX adjuvant and inoculation into rabbits, the trimers rapidly elicited potent autologous tier 2 neutralizing antibodies.					
29881382	9	86	link	derived	1736:1742	arg1	trimers					1744:1750	CHO-M cell derived trimers	1725:1750	CHO-M cell derived trimers	1725:1750	These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers.					
29548671	1	0	theme	HIV-1-envelope	143:156	arg1	trimer					158:163	the HIV-1-envelope trimer	139:163	the HIV-1-envelope trimer	139:163	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	3	1	from	complex	481:487	arg1	fragment					457:464	the antigen-binding fragment	437:464	the antigen-binding fragment of VRC-PG05 in complex with gp120	437:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	5	2	theme	glycan-proximal	862:876	arg1	residue					878:884	glycan-proximal residue E293	862:889	glycan-proximal residue E293	862:889	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	1	3	theme	trimer	158:163	arg1	surface					128:134	Virtually the entire surface	107:134	Virtually the entire surface of the HIV-1-envelope trimer	107:163	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	0	4	theme	HIV	93:95	arg1	Envelope					97:104	the HIV Envelope	89:104	the HIV Envelope	89:104	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope.					
29548671	1	5	from	"	280:280	arg1	subunit					295:301	the gp120 subunit	285:301	the gp120 subunit	285:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	3	6	from	fragment	457:464	arg1	complex					481:487	complex	481:487	complex with gp120	481:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	5	7	theme	N446	842:845	arg1	shifting					815:822	shifting	815:822	shifting of glycan-N448 to N446	815:845	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	5	7	theme	N446	842:845	arg1	mutation					850:857	mutation	850:857	mutation of glycan-proximal residue E293	850:889	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	5	8	dep	N446	842:845	arg1	to					839:840	to	839:840	to	839:840	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	6	9	theme	face	948:951	arg1	center					953:958	at the silent face center	934:958	at the silent face center	934:958	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	6	10	theme	trimer	1143:1148	arg1	regions					1111:1117	all major exposed regions	1093:1117	all major exposed regions of the prefusion closed trimer	1093:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	3	11	theme	antigen-binding	441:455	arg1	fragment					457:464	the antigen-binding fragment	437:464	the antigen-binding fragment of VRC-PG05 in complex with gp120	437:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	12	theme	silent	941:946	arg1	center					953:958	at the silent face center	934:958	at the silent face center	934:958	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	3	13	from	N448	581:584	arg1	glycans					552:558	N-linked glycans	543:558	N-linked glycans from N262, N295, and N448 at the silent face center	543:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	1	14	gly	glycosylated	227:238	arg1	region					240:245	a highly glycosylated region	218:245	a highly glycosylated region at the center of the "silent face" on the gp120 subunit	218:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	4	15	theme	somatic	727:733	arg1	development					735:745	somatic development	727:745	somatic development of glycan recognition	727:767	Somatic hypermutation occurred preferentially at antibody residues that interacted with these glycans, suggesting somatic development of glycan recognition.					
29548671	3	16	from	N262	565:568	arg1	glycans					552:558	N-linked glycans	543:558	N-linked glycans from N262, N295, and N448 at the silent face center	543:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	17	theme	known	1009:1013	arg1	epitopes					1015:1022	other known epitopes	1003:1022	other known epitopes	1003:1022	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	0	18	theme	Envelope	97:104	arg1	Face					81:84	the Silent Face	70:84	the Silent Face of the HIV Envelope	70:104	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope.					
29548671	3	19	link	N-linked	543:550	arg1	glycans					552:558	N-linked glycans	543:558	N-linked glycans from N262, N295, and N448 at the silent face center	543:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	20	theme	prefusion	1126:1134	arg1	trimer					1143:1148	the prefusion closed trimer	1122:1148	the prefusion closed trimer	1122:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	0	21	theme	Neutralizing	2:13	arg1	Antibody					15:22	A Neutralizing Antibody	0:22	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan	0:60	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope.					
29548671	5	22	from	Resistance	770:779	arg1	#					802:802	donor #74	796:804	donor #74	796:804	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	3	23	from	N295	571:574	arg1	glycans					552:558	N-linked glycans	543:558	N-linked glycans from N262, N295, and N448 at the silent face center	543:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	24	theme	at the	934:939	arg1	center					953:958	at the silent face center	934:958	at the silent face center	934:958	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	1	25	theme	neutralizing	182:193	arg1	antibodies					195:204	neutralizing antibodies	182:204	neutralizing antibodies	182:204	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	1	26	from	center	254:259	arg1	subunit					295:301	the gp120 subunit	285:301	the gp120 subunit	285:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	1	26	from	center	254:259	arg1	region					240:245	a highly glycosylated region	218:245	a highly glycosylated region at the center of the "silent face" on the gp120 subunit	218:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	3	27	from	VRC-PG05	469:476	arg1	complex					481:487	complex	481:487	complex with gp120	481:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	28	theme	regions	1111:1117	arg1	antibody					1081:1088	neutralizing antibody	1068:1088	neutralizing antibody of all major exposed regions of the prefusion closed trimer	1068:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	1	29	theme	silent	269:274	arg1	"					280:280	the "silent face"	264:280	the "silent face" on the gp120 subunit	264:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	6	30	theme	closed	1136:1141	arg1	trimer					1143:1148	the prefusion closed trimer	1122:1148	the prefusion closed trimer	1122:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	2	31	theme	HIV-1-infected	312:325	arg1	donor					327:331	an HIV-1-infected donor	309:331	an HIV-1-infected donor	309:331	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	2	31	theme	HIV-1-infected	312:325	arg1	#					334:334	#74	334:336	#74	334:336	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	5	32	theme	donor	796:800	arg1	#					802:802	donor #74	796:804	donor #74	796:804	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	0	33	theme	N-Linked	46:53	arg1	Glycan					55:60	Primarily N-Linked Glycan	36:60	Primarily N-Linked Glycan	36:60	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope.					
29548671	1	34	theme	face	276:279	arg1	"					280:280	the "silent face"	264:280	the "silent face" on the gp120 subunit	264:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	3	35	theme	N-linked	543:550	arg1	glycans					552:558	N-linked glycans	543:558	N-linked glycans from N262, N295, and N448 at the silent face center	543:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	36	theme	other	1003:1007	arg1	epitopes					1015:1022	other known epitopes	1003:1022	other known epitopes	1003:1022	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	3	37	with	complex	481:487	arg1	gp120					494:498	gp120	494:498	gp120	494:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	1	38	theme	"	280:280	arg1	center					254:259	the center	250:259	the center of the "silent face" on the gp120 subunit	250:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	2	39	theme	antibody	353:360	arg1	VRC-PG05					362:369	antibody VRC-PG05	353:369	antibody VRC-PG05	353:369	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	1	40	from	subunit	295:301	arg1	center					254:259	the center	250:259	the center of the "silent face" on the gp120 subunit	250:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	6	41	theme	neutralizing	1068:1079	arg1	antibody					1081:1088	neutralizing antibody	1068:1088	neutralizing antibody of all major exposed regions of the prefusion closed trimer	1068:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	6	42	theme	major	1097:1101	arg1	regions					1111:1117	all major exposed regions	1093:1117	all major exposed regions of the prefusion closed trimer	1093:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	2	43	theme	HIV-1	397:401	arg1	strains					403:409	HIV-1 strains	397:409	HIV-1 strains	397:409	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	6	44	theme	HIV-1	892:896	arg1	neutralization					898:911	HIV-1 neutralization	892:911	HIV-1 neutralization	892:911	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	3	45	theme	face	600:603	arg1	center					605:610	the silent face center	589:610	the silent face center	589:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	3	46	theme	fragment	457:464	arg1	structure					424:432	The crystal structure	412:432	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120	412:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	5	47	theme	residue	878:884	arg1	shifting					815:822	shifting	815:822	shifting of glycan-N448 to N446	815:845	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	5	47	theme	residue	878:884	arg1	mutation					850:857	mutation	850:857	mutation of glycan-proximal residue E293	850:889	Resistance to VRC-PG05 in donor #74 involved shifting of glycan-N448 to N446 or mutation of glycan-proximal residue E293.					
29548671	1	48	theme	gp120	289:293	arg1	subunit					295:301	the gp120 subunit	285:301	the gp120 subunit	285:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	1	49	theme	entire	121:126	arg1	surface					128:134	Virtually the entire surface	107:134	Virtually the entire surface of the HIV-1-envelope trimer	107:163	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	4	50	theme	antibody	662:669	arg1	residues					671:678	antibody residues	662:678	antibody residues that interacted with these glycans	662:713	Somatic hypermutation occurred preferentially at antibody residues that interacted with these glycans, suggesting somatic development of glycan recognition.					
29548671	3	51	theme	crystal	416:422	arg1	structure					424:432	The crystal structure	412:432	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120	412:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	0	52	theme	Silent	74:79	arg1	Face					81:84	the Silent Face	70:84	the Silent Face of the HIV Envelope	70:104	A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope.					
29548671	3	53	theme	silent	593:598	arg1	center					605:610	the silent face center	589:610	the silent face center	589:610	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	4	54	theme	recognition	757:767	arg1	development					735:745	somatic development	727:745	somatic development of glycan recognition	727:767	Somatic hypermutation occurred preferentially at antibody residues that interacted with these glycans, suggesting somatic development of glycan recognition.					
29548671	4	55	theme	Somatic	613:619	arg1	hypermutation					621:633	Somatic hypermutation	613:633	Somatic hypermutation	613:633	Somatic hypermutation occurred preferentially at antibody residues that interacted with these glycans, suggesting somatic development of glycan recognition.					
29548671	1	56	theme	glycosylated	227:238	arg1	region					240:245	a highly glycosylated region	218:245	a highly glycosylated region at the center of the "silent face" on the gp120 subunit	218:301	Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit.					
29548671	2	57	theme	strains	403:409	arg1	%					392:392	27%	390:392	27% of HIV-1 strains	390:409	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	2	57	theme	strains	403:409	arg1	strains					403:409	HIV-1 strains	397:409	HIV-1 strains	397:409	From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which neutralized 27% of HIV-1 strains.					
29548671	6	58	theme	exposed	1103:1109	arg1	regions					1111:1117	all major exposed regions	1093:1117	all major exposed regions of the prefusion closed trimer	1093:1148	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
29548671	4	59	theme	glycan	750:755	arg1	recognition					757:767	glycan recognition	750:767	glycan recognition	750:767	Somatic hypermutation occurred preferentially at antibody residues that interacted with these glycans, suggesting somatic development of glycan recognition.					
29548671	3	60	theme	VRC-PG05	469:476	arg1	fragment					457:464	the antigen-binding fragment	437:464	the antigen-binding fragment of VRC-PG05 in complex with gp120	437:498	The crystal structure of the antigen-binding fragment of VRC-PG05 in complex with gp120 revealed an epitope comprised primarily of N-linked glycans from N262, N295, and N448 at the silent face center.					
29548671	6	61	theme	glycan-recognizing	963:980	arg1	antibody					982:989	glycan-recognizing antibody	963:989	glycan-recognizing antibody	963:989	HIV-1 neutralization can thus be achieved at the silent face center by glycan-recognizing antibody; along with other known epitopes, the VRC-PG05 epitope completes coverage by neutralizing antibody of all major exposed regions of the prefusion closed trimer.					
30177739	5	0	theme	array	784:788	arg1	analyses					790:797	Glycan array analyses	777:797	Glycan array analyses	777:797	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	7	1	gly	non-fucosylated	1317:1331	arg1	glycans					1333:1339	not non-fucosylated glycans	1313:1339	not non-fucosylated glycans	1313:1339	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	7	2	theme	gastrointestinal	1454:1469	arg1	tract					1471:1475	the gastrointestinal tract	1450:1475	the gastrointestinal tract of mammals	1450:1486	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	3	3	theme	structural	496:505	arg1	alignment					507:515	global structural alignment	489:515	global structural alignment	489:515	We recently described a function-prediction technique called SPOT-Struc that identifies CBPs by global structural alignment and binding-affinity prediction.					
30177739	5	4	theme	high	928:931	arg1	affinity					933:940	high affinity	928:940	high affinity binding to Lewisx and lacto-N-fucopentaose III	928:987	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	7	5	theme	mammals	1480:1486	arg1	tract					1471:1475	the gastrointestinal tract	1450:1475	the gastrointestinal tract of mammals	1450:1486	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	5	6	theme	SPR	905:907	arg1	analysis					909:916	SPR analysis	905:916	SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III	905:987	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	6	7	theme	complex	1131:1137	arg1	model					1149:1153	a complex structure model	1129:1153	a complex structure model with predicted binding affinity of -4.3 kcal/mol	1129:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	2	8	from	lack	308:311	arg1	carbohydrates					337:349	carbohydrates	337:349	carbohydrates	337:349	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	6	9	theme	YesU	1019:1022	arg1	alignment					1006:1014	Structure based alignment	990:1014	Structure based alignment of YesU	990:1022	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	10	theme	Lewisx	1103:1108	arg1	docking					1092:1098	docking	1092:1098	docking of Lewisx into YesU	1092:1118	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	7	11	theme	fucosylated	1380:1390	arg1	glycans					1392:1398	fucosylated glycans	1380:1398	fucosylated glycans	1380:1398	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	6	12	dep	lectins	1063:1069	arg1	UEA-I					1071:1075	UEA-I	1071:1075	UEA-I	1071:1075	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	12	dep	lectins	1063:1069	arg1	lectins					1063:1069	the legume lectins UEA-I and GS-IV	1052:1085	the legume lectins UEA-I and GS-IV	1052:1085	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	12	dep	lectins	1063:1069	arg1	GS-IV					1081:1085	GS-IV	1081:1085	GS-IV	1081:1085	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	4	13	theme	high	750:753	arg1	glycans					768:774	high mannose-type glycans	750:774	high mannose-type glycans	750:774	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	6	14	theme	-4.3 kcal/mol	1190:1202	arg1	affinity					1178:1185	predicted binding affinity	1160:1185	predicted binding affinity of -4.3 kcal/mol	1160:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	2	15	theme	high	282:285	arg1	flexibility					287:297	high flexibility	282:297	high flexibility	282:297	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	4	16	theme	binding	617:623	arg1	affinity					625:632	binding affinity	617:632	binding affinity	617:632	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	6	17	theme	structure	1139:1147	arg1	model					1149:1153	a complex structure model	1129:1153	a complex structure model with predicted binding affinity of -4.3 kcal/mol	1129:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	7	18	theme	non-fucosylated	1317:1331	arg1	glycans					1333:1339	not non-fucosylated glycans	1313:1339	not non-fucosylated glycans	1313:1339	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	5	19	theme	-binding	882:889	arg1	lectins					891:897	fucose (Fuc)-binding lectins	870:897	fucose (Fuc)-binding lectins	870:897	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	5	20	theme	binding	823:829	arg1	patterns					831:838	glycan binding patterns	816:838	glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins	816:897	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	2	21	theme	nucleic	365:371	arg1	acids					373:377	nucleic acids	365:377	nucleic acids	365:377	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	7	22	gly	fucosylated	1380:1390	arg1	glycans					1392:1398	fucosylated glycans	1380:1398	fucosylated glycans	1380:1398	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	6	23	theme	binding	1170:1176	arg1	affinity					1178:1185	predicted binding affinity	1160:1185	predicted binding affinity of -4.3 kcal/mol	1160:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	7	24	theme	subtilis	1234:1241	arg1	adherence					1218:1226	the adherence	1214:1226	the adherence of B. subtilis to intestinal cells	1214:1261	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	1	25	theme	carbohydrate-binding	89:108	arg1	proteins					110:117	carbohydrate-binding proteins	89:117	carbohydrate-binding proteins (CBPs)	89:124	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	1	25	theme	carbohydrate-binding	89:108	arg1	CBPs					120:123	CBPs	120:123	CBPs	120:123	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	4	26	theme	uncharacterized	666:680	arg1	YesU					637:640	YesU	637:640	YesU (RCSB PDB ID: 1oq1)	637:660	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	26	theme	uncharacterized	666:680	arg1	protein					682:688	an uncharacterized protein	663:688	an uncharacterized protein from Bacillus subtilis	663:711	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	5	27	theme	similar	840:846	arg1	patterns					831:838	glycan binding patterns	816:838	glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins	816:897	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	6	28	theme	based	1000:1004	arg1	alignment					1006:1014	Structure based alignment	990:1014	Structure based alignment of YesU	990:1022	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	1	29	theme	proteins	110:117	arg1	interaction					74:84	The interaction	70:84	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands	70:164	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	6	30	theme	Structure	990:998	arg1	alignment					1006:1014	Structure based alignment	990:1014	Structure based alignment of YesU	990:1022	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	31	theme	predicted	1160:1168	arg1	affinity					1178:1185	predicted binding affinity	1160:1185	predicted binding affinity of -4.3 kcal/mol	1160:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	4	32	theme	carbohydrate	588:599	arg1	specificity					601:611	carbohydrate specificity	588:611	carbohydrate specificity	588:611	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	33	theme	mannose-type	755:766	arg1	glycans					768:774	high mannose-type glycans	750:774	high mannose-type glycans	750:774	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	34	theme	PDB	648:650	arg1	YesU					637:640	YesU	637:640	YesU (RCSB PDB ID: 1oq1)	637:660	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	34	theme	PDB	648:650	arg1	ID					652:653	RCSB PDB ID	643:653	RCSB PDB ID: 1oq1	643:659	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	1	35	with	interaction	74:84	arg1	ligands					158:164	their corresponding glycan ligands	131:164	their corresponding glycan ligands	131:164	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	3	36	theme	global	489:494	arg1	alignment					507:515	global structural alignment	489:515	global structural alignment	489:515	We recently described a function-prediction technique called SPOT-Struc that identifies CBPs by global structural alignment and binding-affinity prediction.					
30177739	6	37	theme	legume	1056:1061	arg1	UEA-I					1071:1075	UEA-I	1071:1075	UEA-I	1071:1075	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	37	theme	legume	1056:1061	arg1	lectins					1063:1069	the legume lectins UEA-I and GS-IV	1052:1085	the legume lectins UEA-I and GS-IV	1052:1085	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	6	37	theme	legume	1056:1061	arg1	GS-IV					1081:1085	GS-IV	1081:1085	GS-IV	1081:1085	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	5	38	theme	glycan	816:821	arg1	patterns					831:838	glycan binding patterns	816:838	glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins	816:897	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	6	39	with	model	1149:1153	arg1	affinity					1178:1185	predicted binding affinity	1160:1185	predicted binding affinity of -4.3 kcal/mol	1160:1202	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
30177739	4	40	dep	specificity	601:611	arg1	the					584:586	the	584:586	the	584:586	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	41	dep	ID	652:653	arg1	1oq1					656:659	1oq1	656:659	RCSB PDB ID: 1oq1	643:659	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	2	42	theme	sequence	325:332	arg1	affinity					272:279	their low binding affinity	254:279	their low binding affinity	254:279	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	2	42	theme	sequence	325:332	arg1	flexibility					287:297	high flexibility	282:297	high flexibility	282:297	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	2	42	theme	sequence	325:332	arg1	lack					308:311	the lack	304:311	the lack of a linear sequence in carbohydrates	304:349	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	0	43	theme	fucosylated	49:59	arg1	glycans					61:67	fucosylated glycans	49:67	fucosylated glycans	49:67	YesU from Bacillus subtilis preferentially binds fucosylated glycans.					
30177739	2	44	theme	linear	318:323	arg1	sequence					325:332	a linear sequence	316:332	a linear sequence	316:332	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	7	45	theme	subtilis	1438:1445	arg1	adhesion					1423:1430	the adhesion	1419:1430	the adhesion of B. subtilis to the gastrointestinal tract of mammals	1419:1486	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	7	46	theme	YesU	1372:1375	arg1	interaction					1357:1367	the interaction	1353:1367	the interaction of YesU to fucosylated glycans	1353:1398	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	2	47	theme	binding	264:270	arg1	affinity					272:279	their low binding affinity	254:279	their low binding affinity	254:279	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	2	48	from	affinity	272:279	arg1	carbohydrates					337:349	carbohydrates	337:349	carbohydrates	337:349	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	4	49	theme	YesU	637:640	arg1	specificity					601:611	carbohydrate specificity	588:611	carbohydrate specificity	588:611	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	49	theme	YesU	637:640	arg1	affinity					625:632	binding affinity	617:632	binding affinity	617:632	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	2	50	theme	low	260:262	arg1	affinity					272:279	their low binding affinity	254:279	their low binding affinity	254:279	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	1	51	theme	corresponding	137:149	arg1	ligands					158:164	their corresponding glycan ligands	131:164	their corresponding glycan ligands	131:164	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	2	52	from	flexibility	287:297	arg1	carbohydrates					337:349	carbohydrates	337:349	carbohydrates	337:349	This is in part due to their low binding affinity, high flexibility, and the lack of a linear sequence in carbohydrates, as exists in nucleic acids and proteins.					
30177739	7	53	theme	intestinal	1246:1255	arg1	cells					1257:1261	intestinal cells	1246:1261	intestinal cells	1246:1261	Moreover the adherence of B. subtilis to intestinal cells was significantly inhibited by Lex and Ley but by not non-fucosylated glycans, suggesting the interaction of YesU to fucosylated glycans may be involved in the adhesion of B. subtilis to the gastrointestinal tract of mammals.					
30177739	0	54	gly	fucosylated	49:59	arg1	glycans					61:67	fucosylated glycans	49:67	fucosylated glycans	49:67	YesU from Bacillus subtilis preferentially binds fucosylated glycans.					
30177739	1	55	theme	glycan	151:156	arg1	ligands					158:164	their corresponding glycan ligands	131:164	their corresponding glycan ligands	131:164	The interaction of carbohydrate-binding proteins (CBPs) with their corresponding glycan ligands is challenging to study both experimentally and computationally.					
30177739	3	56	theme	function-prediction	417:435	arg1	technique					437:445	a function-prediction technique	415:445	a function-prediction technique called SPOT-Struc that identifies CBPs by global structural alignment and binding-affinity prediction	415:547	We recently described a function-prediction technique called SPOT-Struc that identifies CBPs by global structural alignment and binding-affinity prediction.					
30177739	4	57	theme	RCSB	643:646	arg1	YesU					637:640	YesU	637:640	YesU (RCSB PDB ID: 1oq1)	637:660	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	57	theme	RCSB	643:646	arg1	ID					652:653	RCSB PDB ID	643:653	RCSB PDB ID: 1oq1	643:659	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	5	58	theme	Glycan	777:782	arg1	analyses					790:797	Glycan array analyses	777:797	Glycan array analyses	777:797	Glycan array analyses however revealed glycan binding patterns similar to those exhibited by fucose (Fuc)-binding lectins, with SPR analysis revealing high affinity binding to Lewisx and lacto-N-fucopentaose III.					
30177739	0	59	from	subtilis	19:26	arg1	YesU					0:3	YesU	0:3	YesU from Bacillus subtilis	0:26	YesU from Bacillus subtilis preferentially binds fucosylated glycans.					
30177739	3	60	theme	binding-affinity	521:536	arg1	prediction					538:547	binding-affinity prediction	521:547	binding-affinity prediction	521:547	We recently described a function-prediction technique called SPOT-Struc that identifies CBPs by global structural alignment and binding-affinity prediction.					
30177739	4	61	from	subtilis	704:711	arg1	YesU					637:640	YesU	637:640	YesU (RCSB PDB ID: 1oq1)	637:660	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	4	61	from	subtilis	704:711	arg1	protein					682:688	an uncharacterized protein	663:688	an uncharacterized protein from Bacillus subtilis	663:711	Here we experimentally determined the carbohydrate specificity and binding affinity of YesU (RCSB PDB ID: 1oq1), an uncharacterized protein from Bacillus subtilis that SPOT-Struc predicted would bind high mannose-type glycans.					
30177739	6	62	theme	high	1033:1036	arg1	similarity					1038:1047	high similarity	1033:1047	high similarity to the legume lectins UEA-I and GS-IV	1033:1085	Structure based alignment of YesU revealed high similarity to the legume lectins UEA-I and GS-IV, and docking of Lewisx into YesU revealed a complex structure model with predicted binding affinity of -4.3 kcal/mol.					
29451981	3	0	theme	anion	775:779	arg1	chromatography					797:810	high performance anion exchange (HPAE) chromatography	758:810	high performance anion exchange (HPAE) chromatography	758:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	9	1	theme	sialylation	1554:1564	arg1	levels					1566:1571	sialylation levels	1554:1571	sialylation levels	1554:1571	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	10	2	theme	gradient	1988:1995	arg1	beginning					1971:1979	the beginning	1967:1979	the beginning of the gradient	1967:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	3	3	theme	common	614:619	arg1	workflow					621:628	a common workflow	612:628	a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC)	612:708	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	12	4	theme	site	2285:2288	arg1	location					2299:2306	site specific location	2285:2306	site specific location of the glycans	2285:2321	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	13	5	theme	pharmaceutical	2509:2522	arg1	industry					2524:2531	the pharmaceutical industry	2505:2531	the pharmaceutical industry	2505:2531	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	12	6	theme	workflows	2262:2270	arg1	use					2231:2233	the combined use	2218:2233	the combined use of 2-AB- and native glycan workflows	2218:2270	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	10	7	from	possible	1856:1863	arg1	mode					1874:1877	HILIC mode	1868:1877	HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient	1868:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	8	theme	acetonitrile	1927:1938	arg1	concentrations					1940:1953	the high acetonitrile concentrations	1918:1953	the high acetonitrile concentrations employed at the beginning of the gradient	1918:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	12	9	theme	glycan	2255:2260	arg1	workflows					2262:2270	2-AB- and native glycan workflows	2238:2270	2-AB- and native glycan workflows	2238:2270	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	13	10	theme	regulatory	2478:2487	arg1	conformance					2489:2499	regulatory conformance	2478:2499	regulatory conformance for the pharmaceutical industry	2478:2531	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	1	11	theme	therapeutic	261:271	arg1	N-glycome					246:254	the N-glycome	242:254	the N-glycome of a therapeutic	242:271	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	8	12	theme	dehydration	1413:1423	arg1	artifacts					1425:1433	dehydration artifacts	1413:1433	dehydration artifacts	1413:1433	Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome.					
29451981	0	13	theme	Recombinant	174:184	arg1	Erythropoietin					192:205	a Recombinant Human Erythropoietin	172:205	a Recombinant Human Erythropoietin	172:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	3	14	theme	hydrophilic	663:673	arg1	HILIC					703:707	HILIC	703:707	HILIC	703:707	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	3	14	theme	hydrophilic	663:673	arg1	chromatography					687:700	hydrophilic interaction chromatography	663:700	hydrophilic interaction chromatography (HILIC)	663:708	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	13	15	theme	rhEPO	2450:2454	arg1	N-glycome					2456:2464	the rhEPO N-glycome	2446:2464	the rhEPO N-glycome	2446:2464	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	1	16	dep	modifications	324:336	arg1	acids					380:384	sialic acids	373:384	sialic acids with possible O-acetylation	373:412	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	0	17	theme	Erythropoietin	192:205	arg1	N-Glycome					159:167	the N-Glycome	155:167	the N-Glycome of a Recombinant Human Erythropoietin	155:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	11	18	theme	glycopeptide	2129:2140	arg1	analyses					2142:2149	site specific glycopeptide analyses	2115:2149	site specific glycopeptide analyses	2115:2149	After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses.					
29451981	13	19	theme	N-glycome	2456:2464	arg1	characterization					2426:2441	a more thorough and comprehensive characterization	2392:2441	a more thorough and comprehensive characterization of the rhEPO N-glycome	2392:2464	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	7	20	theme	poor	1269:1272	arg1	performance					1274:1284	poor performance	1269:1284	poor performance	1269:1284	However, it exhibited poor performance toward phosphorylated glycans and did not reveal the presence of sulfated glycans.					
29451981	13	21	theme	comprehensive	2412:2424	arg1	characterization					2426:2441	a more thorough and comprehensive characterization	2392:2441	a more thorough and comprehensive characterization of the rhEPO N-glycome	2392:2464	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	1	22	dep	phosphorylation	345:359	arg1	e.g.					339:342	e.g.	339:342	e.g.	339:342	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	12	23	theme	combined	2222:2229	arg1	use					2231:2233	the combined use	2218:2233	the combined use of 2-AB- and native glycan workflows	2218:2270	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	1	24	theme	possible	391:398	arg1	O-acetylation					400:412	possible O-acetylation	391:412	possible O-acetylation	391:412	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	9	25	theme	lower	1679:1683	arg1	throughput					1685:1694	lower throughput	1679:1694	lower throughput	1679:1694	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	1	26	with	acids	380:384	arg1	O-acetylation					400:412	possible O-acetylation	391:412	possible O-acetylation	391:412	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	4	27	theme	Orbitrap	847:854	arg1	spectrometer					861:872	an Orbitrap mass spectrometer	844:872	an Orbitrap mass spectrometer	844:872	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	0	28	theme	Mass	111:114	arg1	Characterization					135:150	Comprehensive Mass Spectrometry-Based Characterization	97:150	Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin	97:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	10	29	theme	poor	1892:1895	arg1	solubility					1897:1906	to their poor solubility	1883:1906	to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient	1883:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	6	30	theme	reductive	1078:1086	arg1	amination					1088:1096	The reductive amination	1074:1096	The reductive amination based HILIC workflow	1074:1117	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	2	31	theme	chromatographic	464:478	arg1	platforms					480:488	two chromatographic platforms	460:488	two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome	460:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	10	32	theme	based	1788:1792	arg1	possible					1856:1863	possible	1856:1863	possible	1856:1863	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	32	theme	based	1788:1792	arg1	identification					1794:1807	The MS2 based identification	1780:1807	The MS2 based identification of phosphorylated and sulfated glycans	1780:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	8	33	from	profile	1466:1472	arg1	glycome					1487:1493	the rhEPO glycome	1477:1493	the rhEPO glycome	1477:1493	Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome.					
29451981	9	34	theme	artifact	1701:1708	arg1	peaks					1710:1714	artifact peaks	1701:1714	artifact peaks	1701:1714	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	6	35	contain	had	1163:1165	arg2	resolution					1179:1188	good isomer resolution	1167:1188	good isomer resolution	1167:1188	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	6	35	contain	had	1163:1165	arg1	amination					1088:1096	The reductive amination	1074:1096	The reductive amination based HILIC workflow	1074:1117	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	0	36	theme	High	0:3	arg1	Exchange					23:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	6	37	theme	good	1167:1170	arg1	resolution					1179:1188	good isomer resolution	1167:1188	good isomer resolution	1167:1188	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	2	38	theme	platforms	480:488	arg1	comparison					446:455	a comparison	444:455	a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome	444:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	0	39	theme	Anion	17:21	arg1	Exchange					23:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	9	40	theme	epimer	1723:1728	arg1	formation					1730:1738	epimer formation	1723:1738	epimer formation	1723:1738	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	2	41	theme	human	546:550	arg1	rhEPO					568:572	rhEPO	568:572	rhEPO	568:572	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	2	41	theme	human	546:550	arg1	erythropoietin					552:565	a recombinant human erythropoietin	532:565	a recombinant human erythropoietin (rhEPO) N-glycome	532:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	3	42	theme	glycan	732:737	arg1	workflow					739:746	a native N-linked glycan workflow	714:746	a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography	714:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	3	43	theme	native	716:721	arg1	workflow					739:746	a native N-linked glycan workflow	714:746	a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography	714:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	4	44	theme	confident	920:928	arg1	elucidation					941:951	confident structural elucidation	920:951	confident structural elucidation of the glycans	920:966	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	0	45	theme	Interaction	48:58	arg1	Chromatography					67:80	Hydrophilic Interaction Liquid Chromatography	36:80	Hydrophilic Interaction Liquid Chromatography	36:80	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	6	46	theme	sialic	1233:1238	arg1	acids					1240:1244	O-acetylated sialic acids	1220:1244	O-acetylated sialic acids	1220:1244	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	3	47	theme	performance	763:773	arg1	chromatography					797:810	high performance anion exchange (HPAE) chromatography	758:810	high performance anion exchange (HPAE) chromatography	758:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	10	48	theme	glycans	1840:1846	arg1	possible					1856:1863	possible	1856:1863	possible	1856:1863	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	48	theme	glycans	1840:1846	arg1	identification					1794:1807	The MS2 based identification	1780:1807	The MS2 based identification of phosphorylated and sulfated glycans	1780:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	9	49	theme	sialic	1753:1758	arg1	O-acetylation					1765:1777	sialic acid O-acetylation	1753:1777	sialic acid O-acetylation	1753:1777	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	4	50	theme	dependent	884:892	arg1	fragmentation					898:910	data dependent HCD fragmentation	879:910	data dependent HCD fragmentation	879:910	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	2	51	theme	current	420:426	arg1	report					428:433	The current report	416:433	The current report	416:433	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	9	52	theme	charge	1531:1536	arg1	classification					1538:1551	unbiased charge classification	1522:1551	unbiased charge classification	1522:1551	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	9	53	theme	O-acetylation	1765:1777	arg1	throughput					1685:1694	lower throughput	1679:1694	lower throughput	1679:1694	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	9	53	theme	O-acetylation	1765:1777	arg1	loss					1745:1748	loss	1745:1748	loss of sialic acid O-acetylation	1745:1777	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	3	54	theme	HPAE	791:794	arg1	chromatography					797:810	high performance anion exchange (HPAE) chromatography	758:810	high performance anion exchange (HPAE) chromatography	758:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	4	55	theme	glycans	960:966	arg1	elucidation					941:951	confident structural elucidation	920:951	confident structural elucidation of the glycans	920:966	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	7	56	theme	sulfated	1351:1358	arg1	glycans					1360:1366	sulfated glycans	1351:1366	sulfated glycans	1351:1366	However, it exhibited poor performance toward phosphorylated glycans and did not reveal the presence of sulfated glycans.					
29451981	3	57	link	N-linked	723:730	arg1	workflow					739:746	a native N-linked glycan workflow	714:746	a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography	714:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	3	58	theme	exchange	781:788	arg1	chromatography					797:810	high performance anion exchange (HPAE) chromatography	758:810	high performance anion exchange (HPAE) chromatography	758:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	1	59	theme	numerous	315:322	arg1	modifications					324:336	numerous modifications	315:336	numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation)	315:413	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	10	60	theme	to their	1883:1890	arg1	solubility					1897:1906	to their poor solubility	1883:1906	to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient	1883:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	1	61	theme	Comprehensive	208:220	arg1	characterization					222:237	Comprehensive characterization	208:237	Comprehensive characterization of the N-glycome of a therapeutic	208:271	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	12	62	theme	Glycopeptide	2152:2163	arg1	analyses					2165:2172	Glycopeptide analyses	2152:2172	Glycopeptide analyses	2152:2172	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	4	63	theme	structural	930:939	arg1	elucidation					941:951	confident structural elucidation	920:951	confident structural elucidation of the glycans	920:966	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	10	64	theme	high	1922:1925	arg1	concentrations					1940:1953	the high acetonitrile concentrations	1918:1953	the high acetonitrile concentrations employed at the beginning of the gradient	1918:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	9	65	theme	isomer	1584:1589	arg1	resolution					1591:1600	isomer resolution	1584:1600	isomer resolution	1584:1600	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	10	66	from	mode	1874:1877	arg1	identification					1794:1807	The MS2 based identification	1780:1807	The MS2 based identification of phosphorylated and sulfated glycans	1780:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	66	from	mode	1874:1877	arg1	possible					1856:1863	possible	1856:1863	possible	1856:1863	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	12	67	theme	specific	2290:2297	arg1	location					2299:2306	site specific location	2285:2306	site specific location of the glycans	2285:2321	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	0	68	theme	N-Glycome	159:167	arg1	Characterization					135:150	Comprehensive Mass Spectrometry-Based Characterization	97:150	Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin	97:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	1	69	theme	N-glycome	246:254	arg1	characterization					222:237	Comprehensive characterization	208:237	Comprehensive characterization of the N-glycome of a therapeutic	208:271	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	12	70	theme	native	2248:2253	arg1	workflows					2262:2270	2-AB- and native glycan workflows	2238:2270	2-AB- and native glycan workflows	2238:2270	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	3	71	theme	interaction	675:685	arg1	HILIC					703:707	HILIC	703:707	HILIC	703:707	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	3	71	theme	interaction	675:685	arg1	chromatography					687:700	hydrophilic interaction chromatography	663:700	hydrophilic interaction chromatography (HILIC)	663:708	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	9	72	dep	provided	1513:1520	arg1	levels					1566:1571	sialylation levels	1554:1571	sialylation levels	1554:1571	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	8	73	theme	reductive	1382:1390	arg1	amination					1392:1400	reductive amination	1382:1400	reductive amination	1382:1400	Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome.					
29451981	0	74	theme	Human	186:190	arg1	Erythropoietin					192:205	a Recombinant Human Erythropoietin	172:205	a Recombinant Human Erythropoietin	172:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	11	75	theme	glycan	2084:2089	arg1	library					2091:2097	a glycan library	2082:2097	a glycan library	2082:2097	After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses.					
29451981	9	76	theme	phosphorylated	1625:1638	arg1	structures					1653:1662	multiple phosphorylated and sulfated structures	1616:1662	multiple phosphorylated and sulfated structures	1616:1662	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	11	77	theme	specific	2120:2127	arg1	analyses					2142:2149	site specific glycopeptide analyses	2115:2149	site specific glycopeptide analyses	2115:2149	After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses.					
29451981	9	78	theme	sulfated	1644:1651	arg1	structures					1653:1662	multiple phosphorylated and sulfated structures	1616:1662	multiple phosphorylated and sulfated structures	1616:1662	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	12	79	theme	2-AB-	2238:2242	arg1	workflows					2262:2270	2-AB- and native glycan workflows	2238:2270	2-AB- and native glycan workflows	2238:2270	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	0	80	theme	Comprehensive	97:109	arg1	Characterization					135:150	Comprehensive Mass Spectrometry-Based Characterization	97:150	Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin	97:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	6	81	theme	HILIC	1104:1108	arg1	workflow					1110:1117	HILIC workflow	1104:1117	HILIC workflow	1104:1117	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	2	82	theme	erythropoietin	552:565	arg1	N-glycome					575:583	a recombinant human erythropoietin (rhEPO) N-glycome	532:583	a recombinant human erythropoietin (rhEPO) N-glycome	532:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	8	83	theme	glycosylation	1452:1464	arg1	profile					1466:1472	the glycosylation profile	1448:1472	the glycosylation profile in the rhEPO glycome	1448:1493	Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome.					
29451981	0	84	theme	Spectrometry-Based	116:133	arg1	Characterization					135:150	Comprehensive Mass Spectrometry-Based Characterization	97:150	Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin	97:205	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	7	85	theme	phosphorylated	1293:1306	arg1	glycans					1308:1314	phosphorylated glycans	1293:1314	phosphorylated glycans	1293:1314	However, it exhibited poor performance toward phosphorylated glycans and did not reveal the presence of sulfated glycans.					
29451981	2	86	theme	N-glycome	575:583	arg1	characterization					512:527	the comprehensive characterization	494:527	the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome	494:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	4	87	theme	mass	856:859	arg1	spectrometer					861:872	an Orbitrap mass spectrometer	844:872	an Orbitrap mass spectrometer	844:872	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	10	88	theme	MS2	1784:1786	arg1	possible					1856:1863	possible	1856:1863	possible	1856:1863	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	88	theme	MS2	1784:1786	arg1	identification					1794:1807	The MS2 based identification	1780:1807	The MS2 based identification of phosphorylated and sulfated glycans	1780:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	0	89	theme	Performance	5:15	arg1	Exchange					23:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange	0:30	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	6	90	theme	isomer	1172:1177	arg1	resolution					1179:1188	good isomer resolution	1167:1188	good isomer resolution	1167:1188	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	2	91	theme	comprehensive	498:510	arg1	characterization					512:527	the comprehensive characterization	494:527	the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome	494:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	11	92	gly	glycopeptide	2129:2140	arg2	glycopeptide					2129:2140	site specific glycopeptide analyses	2115:2149	site specific glycopeptide analyses	2115:2149	After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses.					
29451981	3	93	theme	N-linked	723:730	arg1	workflow					739:746	a native N-linked glycan workflow	714:746	a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography	714:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	10	94	theme	phosphorylated	1812:1825	arg1	possible					1856:1863	possible	1856:1863	possible	1856:1863	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	10	94	theme	phosphorylated	1812:1825	arg1	identification					1794:1807	The MS2 based identification	1780:1807	The MS2 based identification of phosphorylated and sulfated glycans	1780:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	0	95	theme	Hydrophilic	36:46	arg1	Chromatography					67:80	Hydrophilic Interaction Liquid Chromatography	36:80	Hydrophilic Interaction Liquid Chromatography	36:80	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	13	96	theme	thorough	2399:2406	arg1	characterization					2426:2441	a more thorough and comprehensive characterization	2392:2441	a more thorough and comprehensive characterization of the rhEPO N-glycome	2392:2464	These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry.					
29451981	2	97	theme	recombinant	534:544	arg1	rhEPO					568:572	rhEPO	568:572	rhEPO	568:572	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	2	97	theme	recombinant	534:544	arg1	erythropoietin					552:565	a recombinant human erythropoietin	532:565	a recombinant human erythropoietin (rhEPO) N-glycome	532:583	The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome.					
29451981	4	98	theme	HCD	894:896	arg1	fragmentation					898:910	data dependent HCD fragmentation	879:910	data dependent HCD fragmentation	879:910	Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans.					
29451981	11	99	theme	present	2073:2079	arg1	glycans					2065:2071	the glycans	2061:2071	the glycans present	2061:2079	After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses.					
29451981	0	100	theme	Liquid	60:65	arg1	Chromatography					67:80	Hydrophilic Interaction Liquid Chromatography	36:80	Hydrophilic Interaction Liquid Chromatography	36:80	High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin.					
29451981	6	101	theme	acids	1240:1244	arg1	presence					1208:1215	the presence	1204:1215	the presence of O-acetylated sialic acids	1204:1244	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	3	102	theme	high	758:761	arg1	chromatography					797:810	high performance anion exchange (HPAE) chromatography	758:810	high performance anion exchange (HPAE) chromatography	758:810	The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography.					
29451981	1	103	theme	sialic	373:378	arg1	acids					380:384	sialic acids	373:384	sialic acids with possible O-acetylation	373:412	Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation).					
29451981	6	104	theme	O-acetylated	1220:1231	arg1	acids					1240:1244	O-acetylated sialic acids	1220:1244	O-acetylated sialic acids	1220:1244	The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids.					
29451981	8	105	theme	rhEPO	1481:1485	arg1	glycome					1487:1493	the rhEPO glycome	1477:1493	the rhEPO glycome	1477:1493	Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome.					
29451981	10	106	theme	sulfated	1831:1838	arg1	glycans					1840:1846	sulfated glycans	1831:1846	sulfated glycans	1831:1846	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
29451981	9	107	theme	unbiased	1522:1529	arg1	classification					1538:1551	unbiased charge classification	1522:1551	unbiased charge classification	1522:1551	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	9	108	theme	multiple	1616:1623	arg1	structures					1653:1662	multiple phosphorylated and sulfated structures	1616:1662	multiple phosphorylated and sulfated structures	1616:1662	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	9	109	theme	acid	1760:1763	arg1	O-acetylation					1765:1777	sialic acid O-acetylation	1753:1777	sialic acid O-acetylation	1753:1777	Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation.					
29451981	7	110	theme	glycans	1360:1366	arg1	presence					1339:1346	the presence	1335:1346	the presence of sulfated glycans	1335:1366	However, it exhibited poor performance toward phosphorylated glycans and did not reveal the presence of sulfated glycans.					
29451981	12	111	theme	glycans	2315:2321	arg1	location					2299:2306	site specific location	2285:2306	site specific location of the glycans	2285:2321	Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans.					
29451981	10	112	theme	HILIC	1868:1872	arg1	mode					1874:1877	HILIC mode	1868:1877	HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient	1868:1995	The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient.					
30080931	0	0	theme	influenza	81:89	arg1	development					99:109	H5N1 avian influenza vaccine development	70:109	H5N1 avian influenza vaccine development	70:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	3	1	theme	glycan-masking	734:747	arg1	g127+g138					749:757	the glycan-masking g127+g138	730:757	the glycan-masking g127+g138	730:757	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	2	2	theme	viruses	640:646	arg1	clades/subclades					583:598	heterologous clades/subclades	570:598	heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses	570:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	1	3	link	N-linked	286:293	arg1	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	1	4	theme	undesired	244:252	arg1	sites					264:268	the undesired antigenic sites	240:268	the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen	240:353	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	0	5	theme	avian	75:79	arg1	development					99:109	H5N1 avian influenza vaccine development	70:109	H5N1 avian influenza vaccine development	70:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	2	6	theme	H5N1	635:638	arg1	viruses					640:646	high pathogenic avian influenza H5N1 viruses	603:646	high pathogenic avian influenza H5N1 viruses	603:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	5	7	theme	glycan-masking	949:962	arg1	g127+g138					964:972	the glycan-masking g127+g138	945:972	the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens	945:1009	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	1	8	theme	antigenic	254:262	arg1	sites					264:268	the undesired antigenic sites	240:268	the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen	240:353	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	6	9	theme	glycan-masking	1303:1316	arg1	antigens					1334:1341	only the glycan-masking g127+g138 rH5HA antigens	1294:1341	only the glycan-masking g127+g138 rH5HA antigens	1294:1341	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	2	10	theme	influenza	625:633	arg1	viruses					640:646	high pathogenic avian influenza H5N1 viruses	603:646	high pathogenic avian influenza H5N1 viruses	603:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	6	11	theme	more	1362:1365	arg1	protection					1367:1376	more protection	1362:1376	more protection against live viral challenges	1362:1406	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	4	12	theme	glycan-masking	806:819	arg1	antigens					827:834	these glycan-masking rH5HA antigens	800:834	these glycan-masking rH5HA antigens produced in stable CHO cell clones	800:869	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	0	13	theme	vaccine	91:97	arg1	development					99:109	H5N1 avian influenza vaccine development	70:109	H5N1 avian influenza vaccine development	70:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	3	14	theme	g83+g127+g138	763:775	arg1	antigens					783:790	g83+g127+g138 rH5HA antigens	763:790	g83+g127+g138 rH5HA antigens	763:790	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	2	15	theme	avian	619:623	arg1	viruses					640:646	high pathogenic avian influenza H5N1 viruses	603:646	high pathogenic avian influenza H5N1 viruses	603:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	6	16	theme	live	1386:1389	arg1	challenges					1397:1406	live viral challenges	1386:1406	live viral challenges	1386:1406	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	5	17	theme	neutralizing	1032:1043	arg1	titers					1054:1059	more potent neutralizing antibody titers	1020:1059	more potent neutralizing antibody titers	1020:1059	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	2	18	theme	broader	515:521	arg1	antibodies					536:545	broader neutralizing antibodies	515:545	broader neutralizing antibodies	515:545	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	3	19	theme	rH5HA	777:781	arg1	antigens					783:790	g83+g127+g138 rH5HA antigens	763:790	g83+g127+g138 rH5HA antigens	763:790	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	6	20	theme	antibody	1166:1173	arg1	titers					1175:1180	The increased neutralizing antibody titers	1139:1180	The increased neutralizing antibody titers against these heterologous viral strains	1139:1221	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	5	21	theme	antibody	1045:1052	arg1	titers					1054:1059	more potent neutralizing antibody titers	1020:1059	more potent neutralizing antibody titers	1020:1059	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	2	22	theme	hemagglutin	414:424	arg1	antigens					434:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens	384:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	384:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	2	23	theme	rH5HA	427:431	arg1	antigens					434:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens	384:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	384:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	7	24	theme	cell	1424:1427	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	4	25	theme	rH5HA	821:825	arg1	antigens					827:834	these glycan-masking rH5HA antigens	800:834	these glycan-masking rH5HA antigens produced in stable CHO cell clones	800:869	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	6	26	theme	neutralizing	1153:1164	arg1	titers					1175:1180	The increased neutralizing antibody titers	1139:1180	The increased neutralizing antibody titers against these heterologous viral strains	1139:1221	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	0	27	theme	hemagglutinin	15:27	arg1	antigens					29:36	hemagglutinin antigens	15:36	hemagglutinin antigens	15:36	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	4	28	theme	oligomeric	895:904	arg1	structures					906:915	mostly oligomeric structures	888:915	mostly oligomeric structures	888:915	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	5	29	theme	rH5HA	996:1000	arg1	antigens					1002:1009	g83+g127+g138 rH5HA antigens	982:1009	the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens	945:1009	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	6	30	theme	increased	1143:1151	arg1	titers					1175:1180	The increased neutralizing antibody titers	1139:1180	The increased neutralizing antibody titers against these heterologous viral strains	1139:1221	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	1	31	theme	overall	177:183	arg1	fold					185:188	the overall fold	173:188	the overall fold of the antigen structure	173:213	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	1	32	theme	additional	275:284	arg1	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	5	33	theme	H5N1	1119:1122	arg1	strains					1130:1136	H5N1 viral strains	1119:1136	H5N1 viral strains	1119:1136	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	2	34	theme	H5	411:412	arg1	antigens					434:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens	384:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	384:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	1	35	theme	N-linked	286:293	arg1	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	2	36	theme	heterologous	570:581	arg1	clades/subclades					583:598	heterologous clades/subclades	570:598	heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses	570:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	7	37	theme	subunit	1495:1501	arg1	development					1511:1521	H5N1 subunit vaccine development	1490:1521	H5N1 subunit vaccine development	1490:1521	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	7	38	theme	CHO	1420:1422	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	6	39	theme	increased	1248:1256	arg1	amounts					1258:1264	the increased amounts	1244:1264	the increased amounts of stem-binding antibodies	1244:1291	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	6	39	theme	increased	1248:1256	arg1	antibodies					1282:1291	stem-binding antibodies	1269:1291	stem-binding antibodies	1269:1291	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	1	40	theme	glycosylation	295:307	arg1	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	6	41	theme	stem-binding	1269:1280	arg1	antibodies					1282:1291	stem-binding antibodies	1269:1291	stem-binding antibodies	1269:1291	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	7	42	used	used	1481:1484	arg2	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	6	43	theme	heterologous	1196:1207	arg1	strains					1215:1221	these heterologous viral strains	1190:1221	these heterologous viral strains	1190:1221	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	0	44	theme	CHO	50:52	arg1	clones					59:64	stable CHO cell clones	43:64	stable CHO cell clones for H5N1 avian influenza vaccine development	43:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	7	45	theme	stable	1413:1418	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	7	46	theme	vaccine	1503:1509	arg1	development					1511:1521	H5N1 subunit vaccine development	1490:1521	H5N1 subunit vaccine development	1490:1521	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	7	47	theme	glycan-masking	1443:1456	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	1	48	theme	antigen	197:203	arg1	structure					205:213	the antigen structure	193:213	the antigen structure	193:213	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	0	49	theme	stable	43:48	arg1	clones					59:64	stable CHO cell clones	43:64	stable CHO cell clones for H5N1 avian influenza vaccine development	43:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	7	50	theme	g127+g138	1458:1466	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	4	51	theme	cell	859:862	arg1	clones					864:869	stable CHO cell clones	848:869	stable CHO cell clones	848:869	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	1	52	with	sites	264:268	arg1	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	1	53	theme	structure	205:213	arg1	fold					185:188	the overall fold	173:188	the overall fold of the antigen structure	173:213	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	2	54	theme	pathogenic	608:617	arg1	viruses					640:646	high pathogenic avian influenza H5N1 viruses	603:646	high pathogenic avian influenza H5N1 viruses	603:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	7	55	theme	H5N1	1490:1493	arg1	development					1511:1521	H5N1 subunit vaccine development	1490:1521	H5N1 subunit vaccine development	1490:1521	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	5	56	with	immunization	927:938	arg1	antigens					1002:1009	g83+g127+g138 rH5HA antigens	982:1009	the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens	945:1009	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	5	56	with	immunization	927:938	arg1	g127+g138					964:972	the glycan-masking g127+g138	945:972	the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens	945:1009	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	0	57	theme	cell	54:57	arg1	clones					59:64	stable CHO cell clones	43:64	stable CHO cell clones for H5N1 avian influenza vaccine development	43:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	6	58	theme	antibodies	1282:1291	arg1	amounts					1258:1264	the increased amounts	1244:1264	the increased amounts of stem-binding antibodies	1244:1291	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	6	58	theme	antibodies	1282:1291	arg1	antibodies					1282:1291	stem-binding antibodies	1269:1291	stem-binding antibodies	1269:1291	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	2	59	theme	high	603:606	arg1	viruses					640:646	high pathogenic avian influenza H5N1 viruses	603:646	high pathogenic avian influenza H5N1 viruses	603:646	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	3	60	theme	cell	704:707	arg1	clones					709:714	the stably-expressing CHO cell clones	678:714	the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens	678:790	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	6	61	theme	rH5HA	1328:1332	arg1	antigens					1334:1341	only the glycan-masking g127+g138 rH5HA antigens	1294:1341	only the glycan-masking g127+g138 rH5HA antigens	1294:1341	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	1	62	theme	B	126:126	arg1	responses					133:141	B cell responses	126:141	B cell responses	126:141	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	1	63	theme	vaccine	339:345	arg1	antigen					347:353	the vaccine antigen	335:353	the vaccine antigen	335:353	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	5	64	theme	heterologous	1086:1097	arg1	clades/subclades					1099:1114	four out of five heterologous clades/subclades	1069:1114	four out of five heterologous clades/subclades of H5N1 viral strains	1069:1136	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	5	65	theme	viral	1124:1128	arg1	strains					1130:1136	H5N1 viral strains	1119:1136	H5N1 viral strains	1119:1136	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	1	66	gly	glycosylation	295:307	arg2	motifs					309:314	additional N-linked glycosylation motifs	275:314	additional N-linked glycosylation motifs	275:314	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	1	67	theme	cell	128:131	arg1	responses					133:141	B cell responses	126:141	B cell responses	126:141	Refocusing of B cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan-masking the vaccine antigen.					
30080931	3	68	theme	stably-expressing	682:698	arg1	clones					709:714	the stably-expressing CHO cell clones	678:714	the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens	678:790	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	5	69	theme	potent	1025:1030	arg1	titers					1054:1059	more potent neutralizing antibody titers	1020:1059	more potent neutralizing antibody titers	1020:1059	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	2	70	from	antigens	434:441	arg1	rH5HA					499:503	g127+g138 or g83+g127+138 rH5HA	473:503	g127+g138 or g83+g127+138 rH5HA	473:503	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	2	70	from	antigens	434:441	arg1	residues					446:453	residues 83, 127, and 138	446:470	residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	446:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	6	71	theme	viral	1209:1213	arg1	strains					1215:1221	these heterologous viral strains	1190:1221	these heterologous viral strains	1190:1221	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	7	72	theme	line-produced	1429:1441	arg1	rH5HA					1468:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA	1409:1472	The stable CHO cell line-produced glycan-masking g127+g138 rH5HA can be used for H5N1 subunit vaccine development.					
30080931	5	73	theme	strains	1130:1136	arg1	clades/subclades					1099:1114	four out of five heterologous clades/subclades	1069:1114	four out of five heterologous clades/subclades of H5N1 viral strains	1069:1136	Only the immunization with the glycan-masking g127+g138 but not g83+g127+g138 rH5HA antigens elicited more potent neutralizing antibody titers against four out of five heterologous clades/subclades of H5N1 viral strains.					
30080931	0	74	theme	H5N1	70:73	arg1	development					99:109	H5N1 avian influenza vaccine development	70:109	H5N1 avian influenza vaccine development	70:109	Glycan-masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development.					
30080931	3	75	theme	CHO	700:702	arg1	clones					709:714	the stably-expressing CHO cell clones	678:714	the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens	678:790	In this study, we engineered the stably-expressing CHO cell clones for producing the glycan-masking g127+g138 and g83+g127+g138 rH5HA antigens.					
30080931	4	76	theme	CHO	855:857	arg1	clones					864:869	stable CHO cell clones	848:869	stable CHO cell clones	848:869	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	2	77	theme	recombinant	399:409	arg1	antigens					434:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens	384:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	384:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	4	78	theme	stable	848:853	arg1	clones					864:869	stable CHO cell clones	848:869	stable CHO cell clones	848:869	All of these glycan-masking rH5HA antigens produced in stable CHO cell clones were found to be mostly oligomeric structures.					
30080931	2	79	theme	glycan-masking	384:397	arg1	antigens					434:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens	384:441	glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA)	384:504	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
30080931	6	80	theme	viral	1391:1395	arg1	challenges					1397:1406	live viral challenges	1386:1406	live viral challenges	1386:1406	The increased neutralizing antibody titers against these heterologous viral strains were correlated with the increased amounts of stem-binding antibodies, only the glycan-masking g127+g138 rH5HA antigens can translate into more protection against live viral challenges.					
30080931	2	81	theme	neutralizing	523:534	arg1	antibodies					536:545	broader neutralizing antibodies	515:545	broader neutralizing antibodies	515:545	We previously reported that glycan-masking recombinant H5 hemagglutin (rH5HA) antigens on residues 83, 127, and 138 (g127+g138 or g83+g127+138 rH5HA) elicited broader neutralizing antibodies and protection against heterologous clades/subclades of high pathogenic avian influenza H5N1 viruses.					
29343613	3	0	theme	trimeric	680:687	arg1	spike					689:693	trimeric spike	680:693	trimeric spike	680:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	5	1	with	liabilities	998:1008	arg1	CVN					1015:1017	CVN	1015:1017	CVN	1015:1017	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	6	2	theme	action	1136:1141	arg1	mechanism					1123:1131	a similar mechanism	1113:1131	a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement	1113:1226	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	1	3	theme	DAVEI	189:193	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	3	theme	DAVEI	189:193	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	6	4	theme	gp120	1204:1208	arg1	engagement					1217:1226	more selective gp120 glycan engagement	1189:1226	more selective gp120 glycan engagement	1189:1226	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	3	5	dep	multivalency	584:595	arg1	limit					597:601	limit	597:601	limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	597:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	3	6	theme	glycosylation	547:559	arg1	sites					561:565	multiple glycosylation sites	538:565	multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	538:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	1	7	theme	dual	208:211	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	7	theme	dual	208:211	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	6	8	theme	selective	1194:1202	arg1	engagement					1217:1226	more selective gp120 glycan engagement	1189:1226	more selective gp120 glycan engagement	1189:1226	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	3	9	from	spike	689:693	arg1	understanding					611:623	current understanding	603:623	current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	603:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	11	10	theme	small	1954:1958	arg1	molecules					1960:1968	gp120-binding small molecules	1940:1968	gp120-binding small molecules linked to Trp3 peptide	1940:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	1	11	theme	virus	220:224	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	11	theme	virus	220:224	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	11	12	theme	DAVEI	1915:1919	arg1	fusions					1921:1927	DAVEI fusions	1915:1927	DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide	1915:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	8	13	with	interaction	1410:1420	arg1	gp120					1427:1431	gp120	1427:1431	gp120	1427:1431	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	9	14	theme	lectin	1479:1484	arg1	domain					1486:1491	the lectin domain	1475:1491	the lectin domain in DAVEIs	1475:1501	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	3	15	gly	glycosylation	547:559	arg2	sites					561:565	multiple glycosylation sites	538:565	multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	538:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	11	16	theme	Trp3	1980:1983	arg1	peptide					1985:1991	Trp3 peptide	1980:1991	Trp3 peptide	1980:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	6	17	theme	lytic	1162:1166	arg1	inactivation					1168:1179	HIV-1 lytic inactivation	1156:1179	HIV-1 lytic inactivation	1156:1179	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	0	18	theme	protein	68:74	arg1	chimera					76:82	lectin-reengineered DAVEI protein chimera	42:82	lectin-reengineered DAVEI protein chimera	42:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	3	19	theme	current	603:609	arg1	understanding					611:623	current understanding	603:623	current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	603:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	5	20	theme	single	870:875	arg1	site					892:895	a single glycan-binding site	868:895	a single glycan-binding site in gp120	868:904	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	2	21	theme	gp41	482:485	arg1	engagement					458:467	dual engagement	453:467	dual engagement of gp120 and gp41	453:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	1	22	theme	peptide	336:342	arg1	Trp3					344:347	its derivative peptide Trp3	321:347	its derivative peptide Trp3	321:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	23	theme	first-generation	160:175	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	23	theme	first-generation	160:175	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	4	24	theme	second-generation	760:776	arg1	molecules					784:792	second-generation DAVEI molecules	760:792	second-generation DAVEI molecules	760:792	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
29343613	4	25	theme	molecules	784:792	arg1	function					748:755	the virolytic function	734:755	the virolytic function of second-generation DAVEI molecules	734:792	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
29343613	9	26	theme	virolytic	1535:1543	arg1	function					1545:1552	virolytic function	1535:1552	virolytic function	1535:1552	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	7	27	theme	protein	1309:1315	arg1	potency					1282:1288	the potency	1278:1288	the potency of MVN-DAVEI2-3Trp protein	1278:1315	By sequence redesign, we significantly increased the potency of MVN-DAVEI2-3Trp protein.					
29343613	8	28	theme	MVN-DAVEI2-3Trp	1356:1370	arg1	activity					1393:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	2	29	theme	irreversible	376:387	arg1	inactivation					389:400	potent and irreversible inactivation	365:400	potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41	365:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	0	30	theme	Restricted	0:9	arg1	engagement					28:37	Restricted HIV-1 Env glycan engagement	0:37	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera	0:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	8	31	theme	Q81K/M83R	1372:1380	arg1	activity					1393:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	2	32	theme	immunodeficiency	418:433	arg1	virus					435:439	human immunodeficiency virus	412:439	HIV-1 (human immunodeficiency virus) viruses	405:448	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	0	33	theme	Env	17:19	arg1	engagement					28:37	Restricted HIV-1 Env glycan engagement	0:37	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera	0:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	9	34	theme	restricted	1566:1575	arg1	Env					1583:1585	restricted HIV-1 Env	1566:1585	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	9	34	theme	restricted	1566:1575	arg1	glycoprotein					1597:1608	envelope glycoprotein	1588:1608	envelope glycoprotein	1588:1608	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	2	35	theme	gp120	472:476	arg1	engagement					458:467	dual engagement	453:467	dual engagement of gp120 and gp41	453:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	8	36	theme	virolytic	1383:1391	arg1	activity					1393:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	1	37	theme	proximal	293:300	arg1	region					311:316	a membrane proximal external region	282:316	a membrane proximal external region	282:316	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	9	38	theme	Env	1583:1585	arg1	engagement					1618:1627	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	5	39	with	lectin	856:861	arg1	site					892:895	a single glycan-binding site	868:895	a single glycan-binding site in gp120	868:904	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	3	40	theme	molecular	632:640	arg1	arrangement					642:652	the molecular arrangement	628:652	the molecular arrangement of the DAVEI molecules on trimeric spike	628:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	10	41	theme	multivalent	1690:1700	arg1	binding					1702:1708	DAVEI lectin multivalent binding	1677:1708	DAVEI lectin multivalent binding with gp120	1677:1719	It also shows that DAVEI lectin multivalent binding with gp120 is not required for virolysis.					
29343613	0	42	theme	lectin-reengineered	42:60	arg1	chimera					76:82	lectin-reengineered DAVEI protein chimera	42:82	lectin-reengineered DAVEI protein chimera	42:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	9	43	theme	envelope	1588:1595	arg1	Env					1583:1585	restricted HIV-1 Env	1566:1585	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	9	43	theme	envelope	1588:1595	arg1	glycoprotein					1597:1608	envelope glycoprotein	1588:1608	envelope glycoprotein	1588:1608	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	10	44	theme	DAVEI	1677:1681	arg1	binding					1702:1708	DAVEI lectin multivalent binding	1677:1708	DAVEI lectin multivalent binding with gp120	1677:1719	It also shows that DAVEI lectin multivalent binding with gp120 is not required for virolysis.					
29343613	1	45	theme	entry	226:230	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	45	theme	entry	226:230	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	7	46	theme	sequence	1232:1239	arg1	redesign					1241:1248	sequence redesign	1232:1248	sequence redesign	1232:1248	By sequence redesign, we significantly increased the potency of MVN-DAVEI2-3Trp protein.					
29343613	3	47	theme	DAVEI	661:665	arg1	molecules					667:675	the DAVEI molecules	657:675	the DAVEI molecules	657:675	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	2	48	theme	potent	365:370	arg1	inactivation					389:400	potent and irreversible inactivation	365:400	potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41	365:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	5	49	theme	monovalent	845:854	arg1	MVN					836:838	MVN	836:838	MVN	836:838	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	5	49	theme	monovalent	845:854	arg1	lectin					856:861	a monovalent lectin	843:861	a monovalent lectin	843:861	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	6	50	theme	peptide	1059:1065	arg1	CVN-DAVEI-L2-3Trp					1040:1056	CVN-DAVEI-L2-3Trp	1040:1056	CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW)	1040:1085	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	6	50	theme	peptide	1059:1065	arg1	sequence					1067:1074	peptide sequence DKWASLWNW	1059:1084	peptide sequence DKWASLWNW	1059:1084	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	9	51	gly	glycoprotein	1597:1608	arg1	Env					1583:1585	restricted HIV-1 Env	1566:1585	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	9	51	gly	glycoprotein	1597:1608	arg1	glycoprotein					1597:1608	envelope glycoprotein	1588:1608	envelope glycoprotein	1588:1608	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	3	52	from	arrangement	642:652	arg1	spike					689:693	trimeric spike	680:693	trimeric spike	680:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	3	53	theme	CVN	516:518	arg1	promiscuity					501:511	the promiscuity	497:511	the promiscuity of CVN	497:518	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	1	54	theme	recombinant	177:187	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	54	theme	recombinant	177:187	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	9	55	from	domain	1486:1491	arg1	DAVEIs					1496:1501	DAVEIs	1496:1501	DAVEIs	1496:1501	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	6	56	theme	glycan	1210:1215	arg1	engagement					1217:1226	more selective gp120 glycan engagement	1189:1226	more selective gp120 glycan engagement	1189:1226	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	6	57	theme	similar	1115:1121	arg1	mechanism					1123:1131	a similar mechanism	1113:1131	a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement	1113:1226	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	8	58	with	activity	1393:1400	arg1	gp120					1427:1431	gp120	1427:1431	gp120	1427:1431	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	0	59	theme	virus	128:132	arg1	inactivation					108:119	lytic inactivation	102:119	lytic inactivation of the virus	102:132	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	3	60	theme	multiple	538:545	arg1	sites					561:565	multiple glycosylation sites	538:565	multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	538:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	11	61	theme	Env-DAVEI	1855:1863	arg1	arrangements					1839:1850	productive molecular arrangements	1818:1850	productive molecular arrangements of Env-DAVEI enabling virolysis	1818:1882	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	11	62	theme	gp120-binding	1940:1952	arg1	molecules					1960:1968	gp120-binding small molecules	1940:1968	gp120-binding small molecules linked to Trp3 peptide	1940:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	1	63	theme	action	213:218	arg1	construct					195:203	a first-generation recombinant DAVEI construct	158:203	a first-generation recombinant DAVEI construct	158:203	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	1	63	theme	action	213:218	arg1	inhibitor					232:240	a dual action virus entry inhibitor	206:240	a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3	206:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	11	64	theme	productive	1818:1827	arg1	arrangements					1839:1850	productive molecular arrangements	1818:1850	productive molecular arrangements of Env-DAVEI enabling virolysis	1818:1882	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	11	65	theme	improved	1791:1798	arg1	tool					1800:1803	an improved tool	1788:1803	an improved tool	1788:1803	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	3	66	from	sites	561:565	arg1	gp120					570:574	gp120	570:574	gp120	570:574	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	3	66	from	sites	561:565	arg1	multivalency					584:595	its multivalency	580:595	its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	580:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	11	67	theme	molecular	1829:1837	arg1	arrangements					1839:1850	productive molecular arrangements	1818:1850	productive molecular arrangements of Env-DAVEI enabling virolysis	1818:1882	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	6	68	theme	HIV-1	1156:1160	arg1	inactivation					1168:1179	HIV-1 lytic inactivation	1156:1179	HIV-1 lytic inactivation	1156:1179	We found that, like CVN-DAVEI-L2-3Trp (peptide sequence DKWASLWNW), MVN-DAVEI2-3Trp exploits a similar mechanism of action for inducing HIV-1 lytic inactivation, but by more selective gp120 glycan engagement.					
29343613	11	69	attach	linked	1970:1975	arg1	peptide					1985:1991	Trp3 peptide	1980:1991	Trp3 peptide	1980:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	11	69	attach	linked	1970:1975	arg2	molecules					1960:1968	gp120-binding small molecules	1940:1968	gp120-binding small molecules linked to Trp3 peptide	1940:1991	MVN-DAVEI2-3Trp(Q81K/M83R) provides an improved tool to elucidate productive molecular arrangements of Env-DAVEI enabling virolysis and also opens the way to form DAVEI fusions made up of gp120-binding small molecules linked to Trp3 peptide.					
29343613	1	70	theme	derivative	325:334	arg1	Trp3					344:347	its derivative peptide Trp3	321:347	its derivative peptide Trp3	321:347	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	5	71	theme	glycan-binding	877:890	arg1	site					892:895	a single glycan-binding site	868:895	a single glycan-binding site in gp120	868:904	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	0	72	theme	lytic	102:106	arg1	inactivation					108:119	lytic inactivation	102:119	lytic inactivation of the virus	102:132	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	5	73	from	site	892:895	arg1	gp120					900:904	gp120	900:904	gp120	900:904	MVN is a monovalent lectin with a single glycan-binding site in gp120, is structurally similar to CVN and exhibits no toxicity or mitogenicity, both of which are liabilities with CVN.					
29343613	7	74	theme	MVN-DAVEI2-3Trp	1293:1307	arg1	protein					1309:1315	MVN-DAVEI2-3Trp protein	1293:1315	MVN-DAVEI2-3Trp protein	1293:1315	By sequence redesign, we significantly increased the potency of MVN-DAVEI2-3Trp protein.					
29343613	4	75	theme	DAVEI	778:782	arg1	molecules					784:792	second-generation DAVEI molecules	760:792	second-generation DAVEI molecules	760:792	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
29343613	3	76	from	understanding	611:623	arg1	spike					689:693	trimeric spike	680:693	trimeric spike	680:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	0	77	theme	HIV-1	11:15	arg1	engagement					28:37	Restricted HIV-1 Env glycan engagement	0:37	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera	0:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	9	78	theme	function	1545:1552	arg1	loss					1527:1530	loss	1527:1530	loss of virolytic function	1527:1552	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	2	79	theme	viruses	442:448	arg1	inactivation					389:400	potent and irreversible inactivation	365:400	potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41	365:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	8	80	theme	re-engineered	1342:1354	arg1	activity					1393:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity	1342:1400	Unlike CVN-DAVEI2-3Trp, re-engineered MVN-DAVEI2-3Trp(Q81K/M83R) virolytic activity and its interaction with gp120 were both competed by 2G12 antibody.					
29343613	0	81	theme	glycan	21:26	arg1	engagement					28:37	Restricted HIV-1 Env glycan engagement	0:37	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera	0:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	2	82	theme	virus	435:439	arg1	viruses					442:448	HIV-1 (human immunodeficiency virus) viruses	405:448	HIV-1 (human immunodeficiency virus) viruses	405:448	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	3	83	theme	arrangement	642:652	arg1	understanding					611:623	current understanding	603:623	current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike	603:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	4	84	theme	virolytic	738:746	arg1	function					748:755	the virolytic function	734:755	the virolytic function of second-generation DAVEI molecules	734:792	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
29343613	2	85	theme	human	412:416	arg1	virus					435:439	human immunodeficiency virus	412:439	HIV-1 (human immunodeficiency virus) viruses	405:448	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	1	86	theme	membrane	284:291	arg1	region					311:316	a membrane proximal external region	282:316	a membrane proximal external region	282:316	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	9	87	theme	HIV-1	1577:1581	arg1	Env					1583:1585	restricted HIV-1 Env	1566:1585	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	9	87	theme	HIV-1	1577:1581	arg1	glycoprotein					1597:1608	envelope glycoprotein	1588:1608	envelope glycoprotein	1588:1608	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	2	88	theme	HIV-1	405:409	arg1	viruses					442:448	HIV-1 (human immunodeficiency virus) viruses	405:448	HIV-1 (human immunodeficiency virus) viruses	405:448	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	0	89	theme	DAVEI	62:66	arg1	chimera					76:82	lectin-reengineered DAVEI protein chimera	42:82	lectin-reengineered DAVEI protein chimera	42:82	Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus.					
29343613	1	90	theme	external	302:309	arg1	region					311:316	a membrane proximal external region	282:316	a membrane proximal external region	282:316	We previously reported a first-generation recombinant DAVEI construct, a dual action virus entry inhibitor composed of cyanovirin-N (CVN) fused to a membrane proximal external region or its derivative peptide Trp3.					
29343613	2	91	theme	dual	453:456	arg1	engagement					458:467	dual engagement	453:467	dual engagement of gp120 and gp41	453:485	DAVEI exhibits potent and irreversible inactivation of HIV-1 (human immunodeficiency virus) viruses by dual engagement of gp120 and gp41.					
29343613	3	92	theme	molecules	667:675	arg1	arrangement					642:652	the molecular arrangement	628:652	the molecular arrangement of the DAVEI molecules on trimeric spike	628:693	However, the promiscuity of CVN to associate with multiple glycosylation sites in gp120 and its multivalency limit current understanding of the molecular arrangement of the DAVEI molecules on trimeric spike.					
29343613	10	93	theme	lectin	1683:1688	arg1	binding					1702:1708	DAVEI lectin multivalent binding	1677:1708	DAVEI lectin multivalent binding with gp120	1677:1719	It also shows that DAVEI lectin multivalent binding with gp120 is not required for virolysis.					
29343613	10	94	with	binding	1702:1708	arg1	gp120					1715:1719	gp120	1715:1719	gp120	1715:1719	It also shows that DAVEI lectin multivalent binding with gp120 is not required for virolysis.					
29343613	9	95	theme	glycan	1611:1616	arg1	engagement					1618:1627	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	restricted HIV-1 Env (envelope glycoprotein) glycan engagement	1566:1627	That the lectin domain in DAVEIs can utilize MVN without loss of virolytic function argues that restricted HIV-1 Env (envelope glycoprotein) glycan engagement is sufficient for virolysis.					
29343613	4	96	theme	simpler	802:808	arg1	microvirin					818:827	microvirin	818:827	microvirin (MVN)	818:833	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
29343613	4	96	theme	simpler	802:808	arg1	lectin					810:815	a simpler lectin	800:815	a simpler lectin	800:815	Here, we constructed and investigated the virolytic function of second-generation DAVEI molecules using a simpler lectin, microvirin (MVN).					
30209313	0	0	theme	recombinant	73:83	arg1	trimer					85:90	the virus and soluble recombinant trimer	51:90	trimer	85:90	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	0	1	theme	soluble	65:71	arg1	trimer					85:90	the virus and soluble recombinant trimer	51:90	trimer	85:90	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	0	2	from	processing	13:22	arg1	virus					55:59	the virus and soluble recombinant trimer	51:90	virus	55:59	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	0	2	from	processing	13:22	arg1	trimer					85:90	the virus and soluble recombinant trimer	51:90	trimer	85:90	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	3	3	gly	glycosylation	570:582	arg1	Env					587:589	Env	587:589	Env	587:589	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	4	theme	key	468:470	arg1	aspect					472:477	a key aspect	466:477	a key aspect of HIV vaccine design	466:499	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	5	5	theme	strategy	959:966	arg1	part					928:931	part	928:931	part of an overall HIV vaccine strategy	928:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	5	6	theme	soluble	905:911	arg1	trimers					917:923	soluble Env trimers	905:923	soluble Env trimers as part of an overall HIV vaccine strategy	905:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	3	7	theme	membrane-bound	665:678	arg1	trimers					680:686	corresponding recombinant membrane-bound trimers	639:686	corresponding recombinant membrane-bound trimers	639:686	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	1	8	from	bnAbs	271:275	arg1	humans					280:285	humans	280:285	humans	280:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	5	9	theme	Env	913:915	arg1	trimers					917:923	soluble Env trimers	905:923	soluble Env trimers as part of an overall HIV vaccine strategy	905:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	1	10	theme	vaccination	218:228	arg1	strategies					230:239	vaccination strategies	218:239	vaccination strategies designed to elicit protective bnAbs in humans	218:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	2	11	theme	Env	462:464	arg1	glycosylation					433:445	the glycosylation	429:445	the glycosylation of recombinant Env	429:464	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	5	12	theme	virus	856:860	arg1	glycosylation					866:878	virus Env glycosylation	856:878	virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy	856:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	5	13	theme	trimers	917:923	arg1	design					895:900	the design	891:900	the design of soluble Env trimers as part of an overall HIV vaccine strategy	891:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	4	14	dep	cleaved	756:762	arg1	SOSIP					765:769	SOSIP	765:769	SOSIP	765:769	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	2	15	theme	recombinant	450:460	arg1	Env					462:464	recombinant Env	450:464	recombinant Env	450:464	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	3	16	theme	corresponding	639:651	arg1	trimers					680:686	corresponding recombinant membrane-bound trimers	639:686	corresponding recombinant membrane-bound trimers	639:686	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	3	17	theme	recombinant	653:663	arg1	trimers					680:686	corresponding recombinant membrane-bound trimers	639:686	corresponding recombinant membrane-bound trimers	639:686	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	18	gly	glycosylation	433:445	arg1	Env					462:464	recombinant Env	450:464	recombinant Env	450:464	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	4	19	dep	soluble	747:753	arg1	cleaved					756:762	cleaved	756:762	cleaved	756:762	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	0	20	theme	Differential	0:11	arg1	processing					13:22	Differential processing	0:22	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.	0:91	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	1	21	theme	strategies	230:239	arg1	focus					209:213	the focus	205:213	the focus of vaccination strategies designed to elicit protective bnAbs in humans	205:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	21	theme	strategies	230:239	arg1	glycoprotein					176:187	the envelope glycoprotein	163:187	the envelope glycoprotein (Env) trimer	163:200	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	22	gly	glycoprotein	176:187	arg1	focus					209:213	the focus	205:213	the focus of vaccination strategies designed to elicit protective bnAbs in humans	205:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	22	gly	glycoprotein	176:187	arg1	Env					190:192	Env	190:192	Env	190:192	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	22	gly	glycoprotein	176:187	arg1	glycoprotein					176:187	the envelope glycoprotein	163:187	the envelope glycoprotein (Env) trimer	163:200	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	0	23	theme	HIV	27:29	arg1	glycans					40:46	HIV envelope glycans	27:46	HIV envelope glycans	27:46	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	2	24	theme	vaccine	486:492	arg1	design					494:499	HIV vaccine design	482:499	HIV vaccine design	482:499	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	4	25	theme	viral	698:702	arg1	Envs					704:707	viral Envs	698:707	viral Envs	698:707	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	5	26	theme	HIV	947:949	arg1	strategy					959:966	an overall HIV vaccine strategy	936:966	an overall HIV vaccine strategy	936:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	3	27	from	trimers	680:686	arg1	Envs					629:632	Envs	629:632	Envs from corresponding recombinant membrane-bound trimers	629:686	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	28	theme	HIV	482:484	arg1	design					494:499	HIV vaccine design	482:499	HIV vaccine design	482:499	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	1	29	theme	envelope	167:174	arg1	focus					209:213	the focus	205:213	the focus of vaccination strategies designed to elicit protective bnAbs in humans	205:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	29	theme	envelope	167:174	arg1	Env					190:192	Env	190:192	Env	190:192	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	29	theme	envelope	167:174	arg1	glycoprotein					176:187	the envelope glycoprotein	163:187	the envelope glycoprotein (Env) trimer	163:200	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	0	30	theme	glycans	40:46	arg1	processing					13:22	Differential processing	0:22	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.	0:91	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	5	31	theme	Env	862:864	arg1	glycosylation					866:878	virus Env glycosylation	856:878	virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy	856:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	3	32	from	virus	607:611	arg1	glycosylation					570:582	site-specific glycosylation	556:582	site-specific glycosylation of Env from infectious virus	556:611	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	3	33	theme	HIV	525:527	arg1	strains					529:535	three HIV strains	519:535	three HIV strains	519:535	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	34	theme	most	361:364	arg1	bnAbs					366:370	most bnAbs	361:370	most bnAbs	361:370	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	0	35	theme	envelope	31:38	arg1	glycans					40:46	HIV envelope glycans	27:46	HIV envelope glycans	27:46	Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.					
30209313	1	36	theme	protective	260:269	arg1	bnAbs					271:275	protective bnAbs	260:275	protective bnAbs in humans	260:285	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	3	37	theme	strains	529:535	arg1	analysis					507:514	analysis	507:514	analysis of three HIV strains	507:535	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	38	theme	binding	405:411	arg1	epitope					413:419	their binding epitope	399:419	their binding epitope	399:419	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	3	39	theme	site-specific	556:568	arg1	glycosylation					570:582	site-specific glycosylation	556:582	site-specific glycosylation of Env from infectious virus	556:611	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	4	40	theme	Env	772:774	arg1	trimers					776:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	1	41	theme	sole	100:103	arg1	target					105:110	the sole target	96:110	the sole target of broadly neutralizing antibodies (bnAbs) to HIV	96:160	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	2	42	gly	glycosylated	315:326	arg1	Env					300:302	HIV Env	296:302	HIV Env	296:302	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	3	43	theme	infectious	596:605	arg1	virus					607:611	infectious virus	596:611	infectious virus	596:611	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	2	44	theme	HIV	296:298	arg1	Env					300:302	HIV Env	296:302	HIV Env	296:302	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	4	45	theme	soluble	747:753	arg1	trimers					776:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	1	46	dep	glycoprotein	176:187	arg1	trimer					195:200	trimer	195:200	the envelope glycoprotein (Env) trimer	163:200	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	5	47	theme	vaccine	951:957	arg1	strategy					959:966	an overall HIV vaccine strategy	936:966	an overall HIV vaccine strategy	936:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	2	48	theme	design	494:499	arg1	aspect					472:477	a key aspect	466:477	a key aspect of HIV vaccine design	466:499	Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design.					
30209313	3	49	theme	Env	587:589	arg1	glycosylation					570:582	site-specific glycosylation	556:582	site-specific glycosylation of Env from infectious virus	556:611	Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers.					
30209313	4	50	theme	recombinant	735:745	arg1	trimers					776:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	recombinant soluble, cleaved (SOSIP) Env trimers	735:782	However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity.					
30209313	1	51	theme	neutralizing	123:134	arg1	antibodies					136:145	broadly neutralizing antibodies	115:145	broadly neutralizing antibodies (bnAbs) to HIV	115:160	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	1	51	theme	neutralizing	123:134	arg1	bnAbs					148:152	bnAbs	148:152	bnAbs	148:152	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30209313	5	52	theme	overall	939:945	arg1	strategy					959:966	an overall HIV vaccine strategy	936:966	an overall HIV vaccine strategy	936:966	These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.					
30209313	1	53	theme	antibodies	136:145	arg1	target					105:110	the sole target	96:110	the sole target of broadly neutralizing antibodies (bnAbs) to HIV	96:160	As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans.					
30111543	4	0	theme	increased	766:774	arg1	branching					785:793	increased antennae branching	766:793	increased antennae branching	766:793	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	0	theme	increased	766:774	arg1	differences					745:755	subtle N-glycan differences	729:755	subtle N-glycan differences	729:755	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	6	1	theme	plasma	1185:1190	arg1	proteins					1192:1199	plasma proteins	1185:1199	plasma proteins	1185:1199	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	1	2	theme	various	182:188	arg1	cancers					190:196	various cancers	182:196	various cancers	182:196	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	6	3	theme	proteins	1192:1199	arg1	targets					1225:1231	drug targets	1220:1231	drug targets	1220:1231	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	3	theme	proteins	1192:1199	arg1	many					1202:1205	many	1202:1205	many	1202:1205	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	3	theme	proteins	1192:1199	arg1	pharmacokinetics					1165:1180	the pharmacokinetics	1161:1180	the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states	1161:1297	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	3	theme	proteins	1192:1199	arg1	proteins					1192:1199	plasma proteins	1185:1199	plasma proteins	1185:1199	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	5	4	theme	similar	864:870	arg1	analysis					872:879	similar analysis	864:879	similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex	864:925	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	1	5	theme	cancers	190:196	arg1	disorders					152:160	autoimmune disorders	141:160	autoimmune disorders	141:160	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	1	5	theme	cancers	190:196	arg1	pathogenesis					166:177	pathogenesis	166:177	pathogenesis of various cancers	166:196	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	4	6	theme	dissociation	633:644	arg1	constants					646:654	the dissociation constants	629:654	the dissociation constants of the anticoagulant warfarin to different AGP glycoforms	629:712	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	6	7	theme	drug	1220:1223	arg1	targets					1225:1231	drug targets	1220:1231	drug targets	1220:1231	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	7	theme	drug	1220:1223	arg1	many					1202:1205	many	1202:1205	many	1202:1205	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	7	theme	drug	1220:1223	arg1	proteins					1192:1199	plasma proteins	1185:1199	plasma proteins	1185:1199	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	3	8	theme	spectrometry-based	447:464	arg1	approaches					466:475	mass spectrometry-based approaches	442:475	mass spectrometry-based approaches	442:475	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	1	9	theme	Altered	71:77	arg1	patterns					93:100	Altered glycosylation patterns	71:100	Altered glycosylation patterns of plasma proteins	71:119	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	6	10	theme	disease	1284:1290	arg1	states					1292:1297	various disease states	1276:1297	various disease states	1276:1297	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	4	11	theme	AGP	699:701	arg1	glycoforms					703:712	different AGP glycoforms	689:712	different AGP glycoforms	689:712	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	1	12	theme	glycosylation	79:91	arg1	patterns					93:100	Altered glycosylation patterns	71:100	Altered glycosylation patterns of plasma proteins	71:119	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	2	13	from	changes	263:269	arg1	repertoire					296:305	the glycan structural repertoire	274:305	the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions	274:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	2	14	from	challenge	400:408	arg1	glycobiology					413:424	glycobiology	413:424	glycobiology	413:424	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	5	15	theme	fucosylation	959:970	arg1	effects					948:954	the contrary effects	935:954	the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions	935:1015	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	3	16	theme	site-specific	505:517	arg1	microheterogeneity					519:536	the global and site-specific microheterogeneity	490:536	the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	490:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	5	17	theme	Hp-Hb	998:1002	arg1	interactions					1004:1015	Hp-Hb interactions	998:1015	Hp-Hb interactions	998:1015	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	4	18	gly	glycoforms	703:712	arg1	AGP					699:701	different AGP glycoforms	689:712	different AGP glycoforms	689:712	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	2	19	theme	protein-protein	321:335	arg1	interactions					364:375	protein-protein, or protein-small molecule interactions	321:375	protein-protein, or protein-small molecule interactions	321:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	3	20	theme	mass	442:445	arg1	approaches					466:475	mass spectrometry-based approaches	442:475	mass spectrometry-based approaches	442:475	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	6	21	gly	glycoprotein	1069:1080	arg1	glycoprotein					1069:1080	glycoprotein microheterogeneity	1069:1099	glycoprotein microheterogeneity	1069:1099	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	1	22	theme	plasma	105:110	arg1	proteins					112:119	plasma proteins	105:119	plasma proteins	105:119	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	3	23	theme	global	494:499	arg1	microheterogeneity					519:536	the global and site-specific microheterogeneity	490:536	the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	490:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	0	24	theme	N-glycan	0:7	arg1	microheterogeneity					9:26	N-glycan microheterogeneity	0:26	N-glycan microheterogeneity	0:26	N-glycan microheterogeneity regulates interactions of plasma proteins.					
30111543	6	25	theme	glycoprotein	1069:1080	arg1	microheterogeneity					1082:1099	glycoprotein microheterogeneity	1069:1099	glycoprotein microheterogeneity	1069:1099	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	6	26	theme	various	1276:1282	arg1	states					1292:1297	various disease states	1276:1297	various disease states	1276:1297	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	1	27	theme	proteins	112:119	arg1	patterns					93:100	Altered glycosylation patterns	71:100	Altered glycosylation patterns of plasma proteins	71:119	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	4	28	theme	N-glycan	736:743	arg1	branching					785:793	increased antennae branching	766:793	increased antennae branching	766:793	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	28	theme	N-glycan	736:743	arg1	fucosylation					808:819	terminal fucosylation	799:819	terminal fucosylation	799:819	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	28	theme	N-glycan	736:743	arg1	differences					745:755	subtle N-glycan differences	729:755	subtle N-glycan differences	729:755	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	29	theme	anticoagulant	663:675	arg1	warfarin					677:684	the anticoagulant warfarin	659:684	the anticoagulant warfarin	659:684	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	30	theme	drug-binding	829:840	arg1	affinity					842:849	drug-binding affinity	829:849	drug-binding affinity	829:849	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	3	31	theme	plasma	545:550	arg1	glycoprotein					570:581	α1-acid glycoprotein	562:581	α1-acid glycoprotein (AGP)	562:587	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	31	theme	plasma	545:550	arg1	haptoglobin					593:603	haptoglobin	593:603	haptoglobin (Hp)	593:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	31	theme	plasma	545:550	arg1	proteins					552:559	two plasma proteins	541:559	two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	541:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	32	gly	glycoprotein	570:581	arg1	glycoprotein					570:581	α1-acid glycoprotein	562:581	α1-acid glycoprotein (AGP)	562:587	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	32	gly	glycoprotein	570:581	arg1	AGP					584:586	AGP	584:586	AGP	584:586	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	32	gly	glycoprotein	570:581	arg1	proteins					552:559	two plasma proteins	541:559	two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	541:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	2	33	theme	molecule	355:362	arg1	interactions					364:375	protein-protein, or protein-small molecule interactions	321:375	protein-protein, or protein-small molecule interactions	321:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	3	34	theme	proteins	552:559	arg1	microheterogeneity					519:536	the global and site-specific microheterogeneity	490:536	the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	490:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	0	35	theme	plasma	54:59	arg1	proteins					61:68	plasma proteins	54:68	plasma proteins	54:68	N-glycan microheterogeneity regulates interactions of plasma proteins.					
30111543	4	36	theme	antennae	776:783	arg1	branching					785:793	increased antennae branching	766:793	increased antennae branching	766:793	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	36	theme	antennae	776:783	arg1	differences					745:755	subtle N-glycan differences	729:755	subtle N-glycan differences	729:755	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	2	37	theme	glycoprotein	211:222	arg1	microheterogeneity					224:241	glycoprotein microheterogeneity	211:241	glycoprotein microheterogeneity	211:241	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	2	38	theme	structural	285:294	arg1	repertoire					296:305	the glycan structural repertoire	274:305	the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions	274:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	2	39	from	changes	310:316	arg1	interactions					364:375	protein-protein, or protein-small molecule interactions	321:375	protein-protein, or protein-small molecule interactions	321:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	4	40	theme	subtle	729:734	arg1	branching					785:793	increased antennae branching	766:793	increased antennae branching	766:793	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	40	theme	subtle	729:734	arg1	fucosylation					808:819	terminal fucosylation	799:819	terminal fucosylation	799:819	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	40	theme	subtle	729:734	arg1	differences					745:755	subtle N-glycan differences	729:755	subtle N-glycan differences	729:755	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	6	41	theme	protein-drug/protein	1111:1130	arg1	interactions					1132:1143	protein-drug/protein interactions	1111:1143	protein-drug/protein interactions	1111:1143	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	2	42	theme	glycan	278:283	arg1	repertoire					296:305	the glycan structural repertoire	274:305	the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions	274:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	5	43	theme	contrary	939:946	arg1	effects					948:954	the contrary effects	935:954	the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions	935:1015	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	5	44	theme	Hp-Hb	912:916	arg1	complex					919:925	the haptoglobin-hemoglobin (Hp-Hb) complex	884:925	the haptoglobin-hemoglobin (Hp-Hb) complex	884:925	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	6	45	theme	glycosylation	1244:1256	arg1	status					1258:1263	status	1258:1263	status	1258:1263	Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states.					
30111543	0	46	theme	proteins	61:68	arg1	interactions					38:49	interactions	38:49	interactions of plasma proteins	38:68	N-glycan microheterogeneity regulates interactions of plasma proteins.					
30111543	1	47	theme	autoimmune	141:150	arg1	disorders					152:160	autoimmune disorders	141:160	autoimmune disorders	141:160	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	4	48	theme	terminal	799:806	arg1	fucosylation					808:819	terminal fucosylation	799:819	terminal fucosylation	799:819	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	4	48	theme	terminal	799:806	arg1	differences					745:755	subtle N-glycan differences	729:755	subtle N-glycan differences	729:755	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	2	49	theme	subtle	256:261	arg1	changes					263:269	subtle changes	256:269	subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions	256:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	3	50	gly	microheterogeneity	519:536	arg1	glycoprotein					570:581	α1-acid glycoprotein	562:581	α1-acid glycoprotein (AGP)	562:587	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	50	gly	microheterogeneity	519:536	arg1	haptoglobin					593:603	haptoglobin	593:603	haptoglobin (Hp)	593:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	50	gly	microheterogeneity	519:536	arg1	proteins					552:559	two plasma proteins	541:559	two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	541:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	2	51	theme	significant	388:398	arg1	challenge					400:408	a significant challenge	386:408	a significant challenge in glycobiology	386:424	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	4	52	theme	warfarin	677:684	arg1	constants					646:654	the dissociation constants	629:654	the dissociation constants of the anticoagulant warfarin to different AGP glycoforms	629:712	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	2	53	theme	protein-small	341:353	arg1	interactions					364:375	protein-protein, or protein-small molecule interactions	321:375	protein-protein, or protein-small molecule interactions	321:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	3	54	theme	α1-acid	562:568	arg1	glycoprotein					570:581	α1-acid glycoprotein	562:581	α1-acid glycoprotein (AGP)	562:587	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	54	theme	α1-acid	562:568	arg1	AGP					584:586	AGP	584:586	AGP	584:586	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	54	theme	α1-acid	562:568	arg1	proteins					552:559	two plasma proteins	541:559	two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	541:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	2	55	dep	remains	378:384	arg1	relating					247:254	relating	247:254	relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions	247:375	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	2	55	dep	remains	378:384	arg1	Elucidating					199:209	Elucidating	199:209	Elucidating glycoprotein microheterogeneity	199:241	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
30111543	1	56	gly	glycosylation	79:91	arg1	proteins					112:119	plasma proteins	105:119	plasma proteins	105:119	Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis of various cancers.					
30111543	5	57	theme	branching	985:993	arg1	effects					948:954	the contrary effects	935:954	the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions	935:1015	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	5	58	theme	complex	919:925	arg1	analysis					872:879	similar analysis	864:879	similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex	864:925	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	5	59	theme	N-glycan	976:983	arg1	branching					985:993	N-glycan branching	976:993	N-glycan branching	976:993	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	4	60	theme	different	689:697	arg1	glycoforms					703:712	different AGP glycoforms	689:712	different AGP glycoforms	689:712	We then determine the dissociation constants of the anticoagulant warfarin to different AGP glycoforms and reveal how subtle N-glycan differences, namely, increased antennae branching and terminal fucosylation, reduce drug-binding affinity.					
30111543	3	61	dep	proteins	552:559	arg1	glycoprotein					570:581	α1-acid glycoprotein	562:581	α1-acid glycoprotein (AGP)	562:587	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	61	dep	proteins	552:559	arg1	proteins					552:559	two plasma proteins	541:559	two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp)	541:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	61	dep	proteins	552:559	arg1	Hp					606:607	Hp	606:607	Hp	606:607	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	61	dep	proteins	552:559	arg1	AGP					584:586	AGP	584:586	AGP	584:586	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	3	61	dep	proteins	552:559	arg1	haptoglobin					593:603	haptoglobin	593:603	haptoglobin (Hp)	593:608	Here, we apply mass spectrometry-based approaches to elucidate the global and site-specific microheterogeneity of two plasma proteins: α1-acid glycoprotein (AGP) and haptoglobin (Hp).					
30111543	5	62	from	effects	948:954	arg1	interactions					1004:1015	Hp-Hb interactions	998:1015	Hp-Hb interactions	998:1015	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	5	63	theme	haptoglobin-hemoglobin	888:909	arg1	complex					919:925	the haptoglobin-hemoglobin (Hp-Hb) complex	884:925	the haptoglobin-hemoglobin (Hp-Hb) complex	884:925	Conversely, similar analysis of the haptoglobin-hemoglobin (Hp-Hb) complex reveals the contrary effects of fucosylation and N-glycan branching on Hp-Hb interactions.					
30111543	2	64	gly	glycoprotein	211:222	arg1	glycoprotein					211:222	glycoprotein microheterogeneity	211:241	glycoprotein microheterogeneity	211:241	Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology.					
29618644	14	0	theme	envelope	2663:2670	arg1	spike					2672:2676	a tier 3 envelope spike	2654:2676	a tier 3 envelope spike	2654:2676	These observations provide insight into the distinct molecular features of a tier 3 envelope spike.					
29618644	2	1	theme	other	326:330	arg1	viruses					339:345	other tier 3 viruses	326:345	other tier 3 viruses	326:345	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	8	2	theme	possible	1607:1614	arg1	mechanisms					1616:1625	possible mechanisms	1607:1625	possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1607:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	7	3	theme	close	1356:1360	arg1	proximity					1362:1370	close proximity	1356:1370	close proximity to the trimer axis	1356:1389	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	8	4	theme	spike	1572:1576	arg1	features					1548:1555	the molecular features	1534:1555	the molecular features of an envelope spike from a tier 3 virus	1534:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	8	5	theme	antibody-based	1813:1826	arg1	vaccine					1828:1834	an antibody-based vaccine	1810:1834	an antibody-based vaccine	1810:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	4	6	theme	steric	799:804	arg1	hindrance					817:825	steric or kinetic hindrance	799:825	hindrance	817:825	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	11	7	theme	CD4	2203:2205	arg1	binding					2207:2213	CD4 binding	2203:2213	CD4 binding	2203:2213	Interestingly, we found that these mutations affect the 253-11 envelope (Env) spike before CD4 binding, presumably by destabilizing the trimer structure.					
29618644	3	8	theme	glycosylation	496:508	arg1	sites					510:514	N-linked glycosylation sites	487:514	N-linked glycosylation sites	487:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	4	9	theme	kinetic	809:815	arg1	hindrance					817:825	steric or kinetic hindrance	799:825	hindrance	817:825	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	11	10	theme	spike	2190:2194	arg1	253-11					2168:2173	the 253-11	2164:2173	the 253-11 envelope (Env) spike	2164:2194	Interestingly, we found that these mutations affect the 253-11 envelope (Env) spike before CD4 binding, presumably by destabilizing the trimer structure.					
29618644	3	11	from	polymorphisms	454:466	arg1	sites					510:514	N-linked glycosylation sites	487:514	N-linked glycosylation sites	487:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	3	11	from	polymorphisms	454:466	arg1	loop					478:481	the V3 loop	471:481	the V3 loop	471:481	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	13	12	theme	Env	2563:2565	arg1	structures					2567:2576	most solved HIV-1 Env structures	2545:2576	most solved HIV-1 Env structures	2545:2576	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	3	13	theme	V3	475:476	arg1	loop					478:481	the V3 loop	471:481	the V3 loop	471:481	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	8	14	theme	high	1664:1667	arg1	isolates					1712:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates	1650:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1650:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	7	15	theme	heptad	1313:1318	arg1	helices					1327:1333	the heptad repeat helices	1309:1333	the heptad repeat helices in gp41	1309:1341	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	9	16	theme	elicited	1894:1901	arg1	antibodies					1916:1925	commonly elicited neutralizing antibodies	1885:1925	commonly elicited neutralizing antibodies	1885:1925	We studied 253-11, which is highly resistant to commonly elicited neutralizing antibodies.					
29618644	4	17	theme	external	635:642	arg1	MPER					652:655	MPER	652:655	MPER	652:655	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	4	17	theme	external	635:642	arg1	region					644:649	the 253-11 membrane-proximal external region	606:649	the 253-11 membrane-proximal external region (MPER)	606:656	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	6	18	theme	253-11	1250:1255	arg1	trimer					1263:1268	a recombinant 253-11 SOSIP trimer	1236:1268	a recombinant 253-11 SOSIP trimer	1236:1268	To gain insight into the structure of 253-11, we constructed and crystallized a recombinant 253-11 SOSIP trimer.					
29618644	8	19	theme	resistance.IMPORTANCE	1684:1704	arg1	isolates					1712:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates	1650:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1650:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	1	20	theme	high-coverage	201:213	arg1	vaccine					215:221	a high-coverage vaccine	199:221	a high-coverage vaccine	199:221	Understanding the mechanisms used by HIV-1 to evade antibody neutralization may contribute to the design of a high-coverage vaccine.					
29618644	3	21	theme	neutralization	554:567	arg1	resistance					569:578	the high neutralization resistance	545:578	the high neutralization resistance of 253-11	545:588	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	7	22	from	helices	1327:1333	arg1	gp41					1338:1341	gp41	1338:1341	gp41	1338:1341	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	4	23	theme	native	853:858	arg1	Env					870:872	Env	870:872	Env	870:872	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	4	23	theme	native	853:858	arg1	envelope					860:867	the native envelope	849:867	the native envelope (Env)	849:873	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	10	24	theme	CD4	2099:2101	arg1	binding					2103:2109	CD4 binding	2099:2109	CD4 binding	2099:2109	To further understand its resistance, we made mutations that are known to delay fusion and thus increase the time that the virus spends in the open conformation following CD4 binding.					
29618644	13	25	theme	crystal	2392:2398	arg1	structure					2400:2408	The crystal structure	2388:2408	The crystal structure of the SOSIP trimer	2388:2428	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	5	26	theme	Env	979:981	arg1	conformation					983:994	the prefusion Env conformation	965:994	the prefusion Env conformation	965:994	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	0	27	from	253-11	83:88	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11	0:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	14	28	theme	spike	2672:2676	arg1	features					2642:2649	the distinct molecular features	2619:2649	the distinct molecular features of a tier 3 envelope spike	2619:2676	These observations provide insight into the distinct molecular features of a tier 3 envelope spike.					
29618644	0	29	theme	Tier	63:66	arg1	253-11					83:88	Tier 3 HIV-1 Strain 253-11	63:88	Tier 3 HIV-1 Strain 253-11	63:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	5	30	dep	stable	1125:1130	arg1	closed					1133:1138	closed	1133:1138	closed	1133:1138	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	2	31	dep	subtype-matched	273:287	arg1	sera					303:306	sera	303:306	sera	303:306	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	0	32	theme	HIV-1	70:74	arg1	253-11					83:88	Tier 3 HIV-1 Strain 253-11	63:88	Tier 3 HIV-1 Strain 253-11	63:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	6	33	theme	253-11	1196:1201	arg1	structure					1183:1191	the structure	1179:1191	the structure of 253-11	1179:1201	To gain insight into the structure of 253-11, we constructed and crystallized a recombinant 253-11 SOSIP trimer.					
29618644	3	34	link	N-linked	487:494	arg1	sites					510:514	N-linked glycosylation sites	487:514	N-linked glycosylation sites	487:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	8	35	from	virus	1592:1596	arg1	features					1548:1555	the molecular features	1534:1555	the molecular features of an envelope spike from a tier 3 virus	1534:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	8	35	from	virus	1592:1596	arg1	spike					1572:1576	an envelope spike	1560:1576	an envelope spike from a tier 3 virus	1560:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	0	36	from	Resistance	49:58	arg1	253-11					83:88	Tier 3 HIV-1 Strain 253-11	63:88	Tier 3 HIV-1 Strain 253-11	63:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	0	37	theme	Neutralization	34:47	arg1	Resistance					49:58	Unusually High Neutralization Resistance	19:58	Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11	19:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	14	38	theme	tier	2656:2659	arg1	spike					2672:2676	a tier 3 envelope spike	2654:2676	a tier 3 envelope spike	2654:2676	These observations provide insight into the distinct molecular features of a tier 3 envelope spike.					
29618644	2	39	dep	even	309:312	arg1	viruses					339:345	other tier 3 viruses	326:345	other tier 3 viruses	326:345	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	0	40	theme	Molecular	0:8	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11	0:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	5	41	theme	conformation	983:994	arg1	lifetime					953:960	the lifetime	949:960	the lifetime of the prefusion Env conformation	949:994	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	2	42	theme	virus	235:239	arg1	253-11					241:246	The tier 3 virus 253-11	224:246	The tier 3 virus 253-11	224:246	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	4	43	theme	virus	719:723	arg1	context					694:700	the context	690:700	the context of the wild-type virus	690:723	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	2	44	theme	V3/glycan-targeting	381:399	arg1	MAbs					424:427	MAbs	424:427	MAbs	424:427	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	2	44	theme	V3/glycan-targeting	381:399	arg1	antibodies					412:421	V3/glycan-targeting monoclonal antibodies	381:421	V3/glycan-targeting monoclonal antibodies (MAbs)	381:428	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	14	45	theme	molecular	2632:2640	arg1	features					2642:2649	the distinct molecular features	2619:2649	the distinct molecular features of a tier 3 envelope spike	2619:2676	These observations provide insight into the distinct molecular features of a tier 3 envelope spike.					
29618644	12	46	theme	253-11	2367:2372	arg1	trimer					2380:2385	a recombinant 253-11 SOSIP trimer	2353:2385	a recombinant 253-11 SOSIP trimer	2353:2385	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	4	47	theme	HIV-2	773:777	arg1	chimera					779:785	an HIV-2 chimera	770:785	an HIV-2 chimera	770:785	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	0	48	theme	High	29:32	arg1	Resistance					49:58	Unusually High Neutralization Resistance	19:58	Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11	19:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	5	49	theme	HIV-1	1075:1079	arg1	Env					1081:1083	HIV-1 Env	1075:1083	HIV-1 Env	1075:1083	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	7	50	theme	compact	1441:1447	arg1	disposition					1449:1459	a relatively compact disposition	1428:1459	a relatively compact disposition around the trimer axis	1428:1482	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	12	51	theme	trimer	2332:2337	arg1	structure					2304:2312	the structure	2300:2312	the structure of the 253-11 Env trimer	2300:2337	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	13	52	theme	most	2545:2548	arg1	structures					2567:2576	most solved HIV-1 Env structures	2545:2576	most solved HIV-1 Env structures	2545:2576	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	0	53	theme	Resistance	49:58	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11	0:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	11	54	theme	trimer	2248:2253	arg1	structure					2255:2263	the trimer structure	2244:2263	the trimer structure	2244:2263	Interestingly, we found that these mutations affect the 253-11 envelope (Env) spike before CD4 binding, presumably by destabilizing the trimer structure.					
29618644	8	55	theme	substantial	1509:1519	arg1	insight					1521:1527	substantial insight	1509:1527	substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1509:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	1	56	theme	vaccine	215:221	arg1	design					189:194	the design	185:194	the design of a high-coverage vaccine	185:221	Understanding the mechanisms used by HIV-1 to evade antibody neutralization may contribute to the design of a high-coverage vaccine.					
29618644	8	57	theme	tier	1585:1588	arg1	virus					1592:1596	a tier 3 virus	1583:1596	a tier 3 virus	1583:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	7	58	theme	gp120	1400:1404	arg1	protomers					1406:1414	gp120 protomers	1400:1414	gp120 protomers	1400:1414	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	8	59	theme	molecular	1538:1546	arg1	features					1548:1555	the molecular features	1534:1555	the molecular features of an envelope spike from a tier 3 virus	1534:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	13	60	theme	solved	2550:2555	arg1	structures					2567:2576	most solved HIV-1 Env structures	2545:2576	most solved HIV-1 Env structures	2545:2576	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	13	61	theme	gp41	2448:2451	arg1	helices					2453:2459	the gp41 helices	2444:2459	the gp41 helices	2444:2459	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	2	62	theme	tier	332:335	arg1	viruses					339:345	other tier 3 viruses	326:345	other tier 3 viruses	326:345	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	8	63	theme	vaccine	1828:1834	arg1	efficacy					1798:1805	the efficacy	1794:1805	the efficacy of an antibody-based vaccine	1794:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	5	64	theme	trimer	1140:1145	arg1	structure					1147:1155	its otherwise stable, closed trimer structure	1111:1155	its otherwise stable, closed trimer structure	1111:1155	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	7	65	theme	trimer	1379:1384	arg1	axis					1386:1389	the trimer axis	1375:1389	the trimer axis	1375:1389	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	4	66	theme	binding	830:836	arg1	hindrance					817:825	steric or kinetic hindrance	799:825	hindrance	817:825	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	13	67	theme	gp120	2469:2473	arg1	protomers					2475:2483	the gp120 protomers	2465:2483	the gp120 protomers	2465:2483	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	3	68	gly	glycosylation	496:508	arg2	sites					510:514	N-linked glycosylation sites	487:514	N-linked glycosylation sites	487:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	8	69	theme	envelope	1563:1570	arg1	spike					1572:1576	an envelope spike	1560:1576	an envelope spike from a tier 3 virus	1560:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	13	70	theme	trimer	2423:2428	arg1	structure					2400:2408	The crystal structure	2388:2408	The crystal structure of the SOSIP trimer	2388:2428	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	3	71	theme	N-linked	487:494	arg1	sites					510:514	N-linked glycosylation sites	487:514	N-linked glycosylation sites	487:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	13	72	theme	HIV-1	2557:2561	arg1	structures					2567:2576	most solved HIV-1 Env structures	2545:2576	most solved HIV-1 Env structures	2545:2576	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	8	73	dep	antibodies	1771:1780	arg1	limit					1788:1792	limit	1788:1792	could limit the efficacy of an antibody-based vaccine	1782:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	8	74	from	features	1548:1555	arg1	virus					1592:1596	a tier 3 virus	1583:1596	a tier 3 virus	1583:1596	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	2	75	theme	subtype	293:299	arg1	C					301:301	subtype C	293:301	subtype C	293:301	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	10	76	theme	open	2071:2074	arg1	conformation					2076:2087	the open conformation	2067:2087	the open conformation	2067:2087	To further understand its resistance, we made mutations that are known to delay fusion and thus increase the time that the virus spends in the open conformation following CD4 binding.					
29618644	6	77	theme	SOSIP	1257:1261	arg1	trimer					1263:1268	a recombinant 253-11 SOSIP trimer	1236:1268	a recombinant 253-11 SOSIP trimer	1236:1268	To gain insight into the structure of 253-11, we constructed and crystallized a recombinant 253-11 SOSIP trimer.					
29618644	2	78	theme	tier	228:231	arg1	253-11					241:246	The tier 3 virus 253-11	224:246	The tier 3 virus 253-11	224:246	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	12	79	theme	further	2274:2280	arg1	information					2282:2292	further information	2274:2292	further information about the structure of the 253-11 Env trimer	2274:2337	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	7	80	theme	repeat	1320:1325	arg1	helices					1327:1333	the heptad repeat helices	1309:1333	the heptad repeat helices in gp41	1309:1341	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	9	81	theme	neutralizing	1903:1914	arg1	antibodies					1916:1925	commonly elicited neutralizing antibodies	1885:1925	commonly elicited neutralizing antibodies	1885:1925	We studied 253-11, which is highly resistant to commonly elicited neutralizing antibodies.					
29618644	4	82	theme	membrane-proximal	617:633	arg1	MPER					652:655	MPER	652:655	MPER	652:655	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	4	82	theme	membrane-proximal	617:633	arg1	region					644:649	the 253-11 membrane-proximal external region	606:649	the 253-11 membrane-proximal external region (MPER)	606:656	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	6	83	theme	recombinant	1238:1248	arg1	trimer					1263:1268	a recombinant 253-11 SOSIP trimer	1236:1268	a recombinant 253-11 SOSIP trimer	1236:1268	To gain insight into the structure of 253-11, we constructed and crystallized a recombinant 253-11 SOSIP trimer.					
29618644	3	84	theme	high	549:552	arg1	resistance					569:578	the high neutralization resistance	545:578	the high neutralization resistance of 253-11	545:588	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	8	85	theme	neutralization	1669:1682	arg1	isolates					1712:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates	1650:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1650:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	5	86	theme	prefusion	969:977	arg1	conformation					983:994	the prefusion Env conformation	965:994	the prefusion Env conformation	965:994	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	8	87	theme	HIV-1	1706:1710	arg1	isolates					1712:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates	1650:1719	its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1650:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	4	88	from	MPER	841:844	arg1	Env					870:872	Env	870:872	Env	870:872	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	4	88	from	MPER	841:844	arg1	envelope					860:867	the native envelope	849:867	the native envelope (Env)	849:873	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	3	89	theme	253-11	583:588	arg1	resistance					569:578	the high neutralization resistance	545:578	the high neutralization resistance of 253-11	545:588	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	7	90	theme	resulting	1275:1283	arg1	structure					1285:1293	The resulting structure	1271:1293	The resulting structure	1271:1293	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	0	91	theme	Strain	76:81	arg1	253-11					83:88	Tier 3 HIV-1 Strain 253-11	63:88	Tier 3 HIV-1 Strain 253-11	63:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	5	92	theme	various	1055:1061	arg1	epitopes					1063:1070	various epitopes	1055:1070	various epitopes on HIV-1 Env	1055:1083	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	1	93	theme	antibody	143:150	arg1	neutralization					152:165	antibody neutralization	143:165	antibody neutralization	143:165	Understanding the mechanisms used by HIV-1 to evade antibody neutralization may contribute to the design of a high-coverage vaccine.					
29618644	0	94	from	Basis	10:14	arg1	253-11					83:88	Tier 3 HIV-1 Strain 253-11	63:88	Tier 3 HIV-1 Strain 253-11	63:88	Molecular Basis of Unusually High Neutralization Resistance in Tier 3 HIV-1 Strain 253-11.					
29618644	5	95	theme	253-11	893:898	arg1	MPER					900:903	the 253-11 MPER	889:903	the 253-11 MPER	889:903	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	7	96	theme	trimer	1472:1477	arg1	axis					1479:1482	the trimer axis	1468:1482	the trimer axis	1468:1482	The resulting structure revealed that the heptad repeat helices in gp41 are drawn in close proximity to the trimer axis and that gp120 protomers also showed a relatively compact disposition around the trimer axis.					
29618644	13	97	theme	molecule	2524:2531	arg1	center					2510:2515	the center	2506:2515	the center of the molecule	2506:2531	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	5	98	theme	253-11	1024:1029	arg1	resistance					1010:1019	the resistance	1006:1019	the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env	1006:1083	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	8	99	theme	neutralizing	1758:1769	arg1	antibodies					1771:1780	broadly neutralizing antibodies	1750:1780	broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine	1750:1834	These observations give substantial insight into the molecular features of an envelope spike from a tier 3 virus and into possible mechanisms that may contribute to its unusually high neutralization resistance.IMPORTANCE HIV-1 isolates that are highly resistant to broadly neutralizing antibodies could limit the efficacy of an antibody-based vaccine.					
29618644	12	100	theme	recombinant	2355:2365	arg1	trimer					2380:2385	a recombinant 253-11 SOSIP trimer	2353:2385	a recombinant 253-11 SOSIP trimer	2353:2385	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	2	101	theme	monoclonal	401:410	arg1	MAbs					424:427	MAbs	424:427	MAbs	424:427	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	2	101	theme	monoclonal	401:410	arg1	antibodies					412:421	V3/glycan-targeting monoclonal antibodies	381:421	V3/glycan-targeting monoclonal antibodies (MAbs)	381:428	The tier 3 virus 253-11 is poorly neutralized by subtype-matched and subtype C sera, even compared to other tier 3 viruses, and is also recognized poorly by V3/glycan-targeting monoclonal antibodies (MAbs).					
29618644	12	102	theme	SOSIP	2374:2378	arg1	trimer					2380:2385	a recombinant 253-11 SOSIP trimer	2353:2385	a recombinant 253-11 SOSIP trimer	2353:2385	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	4	103	theme	wild-type	709:717	arg1	virus					719:723	the wild-type virus	705:723	the wild-type virus	705:723	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	5	104	from	epitopes	1063:1070	arg1	Env					1081:1083	HIV-1 Env	1075:1083	HIV-1 Env	1075:1083	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	5	105	theme	stable	1125:1130	arg1	structure					1147:1155	its otherwise stable, closed trimer structure	1111:1155	its otherwise stable, closed trimer structure	1111:1155	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	5	106	from	Mutations	876:884	arg1	MPER					900:903	the 253-11 MPER	889:903	the 253-11 MPER	889:903	Mutations in the 253-11 MPER, which were previously reported to increase the lifetime of the prefusion Env conformation, affected the resistance of 253-11 to antibodies targeting various epitopes on HIV-1 Env, presumably destabilizing its otherwise stable, closed trimer structure.					
29618644	14	107	theme	distinct	2623:2630	arg1	features					2642:2649	the distinct molecular features	2619:2649	the distinct molecular features of a tier 3 envelope spike	2619:2676	These observations provide insight into the distinct molecular features of a tier 3 envelope spike.					
29618644	13	108	theme	SOSIP	2417:2421	arg1	trimer					2423:2428	the SOSIP trimer	2413:2428	the SOSIP trimer	2413:2428	The crystal structure of the SOSIP trimer revealed that the gp41 helices and the gp120 protomers were drawn in toward the center of the molecule compared to most solved HIV-1 Env structures.					
29618644	4	109	theme	chimera	779:785	arg1	context					759:765	the context	755:765	the context of an HIV-2 chimera	755:785	Interestingly, the 253-11 membrane-proximal external region (MPER) is rarely recognized by sera in the context of the wild-type virus but is commonly recognized in the context of an HIV-2 chimera, suggesting steric or kinetic hindrance of binding to MPER in the native envelope (Env).					
29618644	3	110	theme	sequence	445:452	arg1	polymorphisms					454:466	sequence polymorphisms	445:466	sequence polymorphisms in the V3 loop and N-linked glycosylation sites	445:514	We found that sequence polymorphisms in the V3 loop and N-linked glycosylation sites contribute only minimally to the high neutralization resistance of 253-11.					
29618644	12	111	theme	Env	2328:2330	arg1	trimer					2332:2337	the 253-11 Env trimer	2317:2337	the 253-11 Env trimer	2317:2337	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29618644	12	112	theme	253-11	2321:2326	arg1	trimer					2332:2337	the 253-11 Env trimer	2317:2337	the 253-11 Env trimer	2317:2337	To gain further information about the structure of the 253-11 Env trimer, we generated a recombinant 253-11 SOSIP trimer.					
29802667	4	0	theme	chemoenzymatic	464:477	arg1	strategy					479:486	This chemoenzymatic strategy	459:486	This chemoenzymatic strategy	459:486	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	1	1	dep	30 	88:90	arg1	to					85:86	to	85:86	to	85:86	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	5	2	theme	binding	764:770	arg1	profiles					772:779	The binding profiles	760:779	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins	760:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	4	3	theme	O-mannose	688:696	arg1	glycans					698:704	58 complex O-mannose glycans	677:704	58 complex O-mannose glycans	677:704	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	4	theme	structures	565:574	arg1	synthesis					521:529	the gram-scale chemical synthesis	497:529	the gram-scale chemical synthesis of five judiciously designed core structures	497:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	4	theme	structures	565:574	arg1	modification					604:615	the diversity-oriented modification	581:615	the diversity-oriented modification of the core structures with three enzyme modules	581:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	3	5	theme	efficient	322:330	arg1	strategy					347:354	a highly efficient chemoenzymatic strategy	313:354	a highly efficient chemoenzymatic strategy	313:354	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	4	6	with	modification	604:615	arg1	modules					658:664	three enzyme modules	645:664	three enzyme modules	645:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	1	7	theme	total	96:100	arg1	O-glycans					102:110	total O-glycans	96:110	total O-glycans	96:110	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	3	8	theme	chemoenzymatic	332:345	arg1	strategy					347:354	a highly efficient chemoenzymatic strategy	313:354	a highly efficient chemoenzymatic strategy	313:354	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	4	9	theme	linear	711:716	arg1	sequence					718:725	a linear sequence	709:725	a linear sequence that does not exceed four steps	709:757	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	10	theme	core	624:627	arg1	structures					629:638	the core structures	620:638	the core structures	620:638	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	2	11	theme	causative	250:258	arg1	factor					260:265	a causative factor	248:265	a causative factor for various muscular diseases	248:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	2	11	theme	causative	250:258	arg1	α-dystroglycan					222:235	α-dystroglycan	222:235	α-dystroglycan	222:235	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	5	12	theme	antibodies	837:846	arg1	panel					819:823	a panel	817:823	a panel of lectins, antibodies, and brain proteins	817:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	2	13	theme	Previous	126:133	arg1	synthesis					135:143	Previous synthesis and functional studies	126:166	synthesis	135:143	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	3	14	theme	first	387:391	arg1	synthesis					404:412	the first collective synthesis	383:412	the first collective synthesis of 63 core M1 and core M2 O-mannose glycans	383:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	5	15	theme	glycans	804:810	arg1	profiles					772:779	The binding profiles	760:779	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins	760:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	3	16	theme	core	420:423	arg1	M1					425:426	63 core M1 and core M2 O-mannose glycans	417:456	M1	425:426	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	5	17	theme	O-mannose	794:802	arg1	glycans					804:810	synthetic O-mannose glycans	784:810	synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins	784:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	4	18	theme	enzyme	651:656	arg1	modules					658:664	three enzyme modules	645:664	three enzyme modules	645:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	5	19	theme	O-mannose	906:914	arg1	array					923:927	a printed O-mannose glycan array	896:927	a printed O-mannose glycan array	896:927	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	0	20	theme	Chemoenzymatic	0:13	arg1	Assembly					15:22	Chemoenzymatic Assembly	0:22	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans	0:53	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans.					
29802667	4	21	with	synthesis	521:529	arg1	modules					658:664	three enzyme modules	645:664	three enzyme modules	645:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	5	22	theme	brain	853:857	arg1	proteins					859:866	brain proteins	853:866	brain proteins	853:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	0	23	theme	Mammalian	27:35	arg1	Glycans					47:53	Mammalian O-Mannose Glycans	27:53	Mammalian O-Mannose Glycans	27:53	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans.					
29802667	5	24	with	glycans	804:810	arg1	panel					819:823	a panel	817:823	a panel of lectins, antibodies, and brain proteins	817:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	5	25	theme	printed	898:904	arg1	array					923:927	a printed O-mannose glycan array	896:927	a printed O-mannose glycan array	896:927	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	2	26	theme	muscular	279:286	arg1	diseases					288:295	various muscular diseases	271:295	various muscular diseases	271:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	3	27	theme	core	432:435	arg1	glycans					450:456	63 core M1 and core M2 O-mannose glycans	417:456	glycans	450:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	5	28	theme	glycan	916:921	arg1	array					923:927	a printed O-mannose glycan array	896:927	a printed O-mannose glycan array	896:927	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	4	29	theme	core	560:563	arg1	structures					565:574	five judiciously designed core structures	534:574	five judiciously designed core structures	534:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	2	30	theme	various	271:277	arg1	diseases					288:295	various muscular diseases	271:295	various muscular diseases	271:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	5	31	theme	proteins	859:866	arg1	panel					819:823	a panel	817:823	a panel of lectins, antibodies, and brain proteins	817:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	3	32	theme	M2	437:438	arg1	glycans					450:456	63 core M1 and core M2 O-mannose glycans	417:456	glycans	450:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	0	33	theme	Glycans	47:53	arg1	Assembly					15:22	Chemoenzymatic Assembly	0:22	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans	0:53	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans.					
29802667	4	34	theme	designed	551:558	arg1	structures					565:574	five judiciously designed core structures	534:574	five judiciously designed core structures	534:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	5	35	theme	lectins	828:834	arg1	panel					819:823	a panel	817:823	a panel of lectins, antibodies, and brain proteins	817:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	3	36	theme	M1	425:426	arg1	synthesis					404:412	the first collective synthesis	383:412	the first collective synthesis of 63 core M1 and core M2 O-mannose glycans	383:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	0	37	theme	O-Mannose	37:45	arg1	Glycans					47:53	Mammalian O-Mannose Glycans	27:53	Mammalian O-Mannose Glycans	27:53	Chemoenzymatic Assembly of Mammalian O-Mannose Glycans.					
29802667	4	38	theme	chemical	512:519	arg1	synthesis					521:529	the gram-scale chemical synthesis	497:529	the gram-scale chemical synthesis of five judiciously designed core structures	497:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	3	39	theme	collective	393:402	arg1	synthesis					404:412	the first collective synthesis	383:412	the first collective synthesis of 63 core M1 and core M2 O-mannose glycans	383:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	4	40	theme	gram-scale	501:510	arg1	synthesis					521:529	the gram-scale chemical synthesis	497:529	the gram-scale chemical synthesis of five judiciously designed core structures	497:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	2	41	theme	functional	149:158	arg1	studies					160:166	Previous synthesis and functional studies	126:166	studies	160:166	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	2	42	theme	O-mannose	201:209	arg1	glycans					211:217	the core M3 O-mannose glycans	189:217	the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases	189:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	1	43	theme	O-glycans	102:110	arg1	%					91:91	up to 30 %	82:91	up to 30 % of total O-glycans in the brain	82:123	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	1	43	theme	O-glycans	102:110	arg1	O-glycans					102:110	total O-glycans	96:110	total O-glycans	96:110	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	5	44	theme	synthetic	784:792	arg1	glycans					804:810	synthetic O-mannose glycans	784:810	synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins	784:866	The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.					
29802667	2	45	theme	core	193:196	arg1	glycans					211:217	the core M3 O-mannose glycans	189:217	the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases	189:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	2	46	theme	M3	198:199	arg1	glycans					211:217	the core M3 O-mannose glycans	189:217	the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases	189:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
29802667	1	47	theme	O-Mannose	56:64	arg1	glycans					66:72	O-Mannose glycans	56:72	O-Mannose glycans	56:72	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	3	48	theme	O-mannose	440:448	arg1	glycans					450:456	63 core M1 and core M2 O-mannose glycans	417:456	glycans	450:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	3	49	theme	glycans	450:456	arg1	synthesis					404:412	the first collective synthesis	383:412	the first collective synthesis of 63 core M1 and core M2 O-mannose glycans	383:456	In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans.					
29802667	4	50	theme	diversity-oriented	585:602	arg1	modification					604:615	the diversity-oriented modification	581:615	the diversity-oriented modification of the core structures with three enzyme modules	581:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	51	theme	structures	629:638	arg1	synthesis					521:529	the gram-scale chemical synthesis	497:529	the gram-scale chemical synthesis of five judiciously designed core structures	497:574	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	51	theme	structures	629:638	arg1	modification					604:615	the diversity-oriented modification	581:615	the diversity-oriented modification of the core structures with three enzyme modules	581:664	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	4	52	theme	complex	680:686	arg1	glycans					698:704	58 complex O-mannose glycans	677:704	58 complex O-mannose glycans	677:704	This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps.					
29802667	1	53	from	%	91:91	arg1	brain					119:123	the brain	115:123	the brain	115:123	O-Mannose glycans account up to 30 % of total O-glycans in the brain.					
29802667	2	54	theme	α-dystroglycan	222:235	arg1	glycans					211:217	the core M3 O-mannose glycans	189:217	the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases	189:295	Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of α-dystroglycan, which are a causative factor for various muscular diseases.					
30311906	0	0	theme	developing	80:89	arg1	cerebellum					91:100	the developing cerebellum	76:100	the developing cerebellum	76:100	High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.					
30311906	7	1	theme	Srd5a3	1153:1158	arg1	cerebellum					1167:1176	Srd5a3 mutant cerebellum	1153:1176	Srd5a3 mutant cerebellum	1153:1176	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	7	2	theme	IgSF-CAM	988:995	arg1	proteins					1006:1013	IgSF-CAM adhesion proteins	988:1013	IgSF-CAM adhesion proteins	988:1013	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	5	3	dep	defects	640:646	arg1	addition					609:616	addition	609:616	addition	609:616	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	8	4	theme	brain	1275:1279	arg1	development					1281:1291	mammalian brain development	1265:1291	mammalian brain development	1265:1291	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	5	5	theme	mild	710:713	arg1	impairment					731:740	mild N-glycosylation impairment	710:740	mild N-glycosylation impairment	710:740	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	1	6	theme	protein	171:177	arg1	N-glycosylation					179:193	protein N-glycosylation	171:193	protein N-glycosylation	171:193	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	2	7	theme	congenital	369:378	arg1	disorders					380:388	congenital disorders	369:388	congenital disorders of glycosylation	369:405	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	5	8	theme	N-glycosylation	715:729	arg1	impairment					731:740	mild N-glycosylation impairment	710:740	mild N-glycosylation impairment	710:740	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	5	9	theme	abnormal	652:659	arg1	development					674:684	abnormal granule cell development	652:684	abnormal granule cell development	652:684	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	2	10	theme	broad	327:331	arg1	symptoms					346:353	broad neurological symptoms	327:353	broad neurological symptoms identified in congenital disorders of glycosylation	327:405	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	7	11	gly	N-glycosylated	1059:1072	arg1	proteins					1006:1013	IgSF-CAM adhesion proteins	988:1013	IgSF-CAM adhesion proteins	988:1013	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	5	12	theme	granule	661:667	arg1	development					674:684	abnormal granule cell development	652:684	abnormal granule cell development	652:684	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	6	13	theme	proteomic	795:803	arg1	approaches					805:814	proteomic approaches	795:814	proteomic approaches	795:814	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	4	14	theme	knockout	525:532	arg1	mouse					534:538	a cerebellum specific knockout mouse	503:538	a cerebellum specific knockout mouse for Srd5a3, a gene involved in the initiation of N-glycosylation	503:603	We generated a cerebellum specific knockout mouse for Srd5a3, a gene involved in the initiation of N-glycosylation.					
30311906	5	15	theme	cell	669:672	arg1	development					674:684	abnormal granule cell development	652:684	abnormal granule cell development	652:684	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	2	16	theme	early	306:310	arg1	steps					312:316	early steps	306:316	early steps	306:316	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	0	17	theme	N-glycosylation	105:119	arg1	defect					121:126	N-glycosylation defect	105:126	N-glycosylation defect	105:126	High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.					
30311906	6	18	theme	protein	941:947	arg1	abundance					949:957	protein abundance	941:957	protein abundance	941:957	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	0	19	theme	N-glycan	5:12	arg1	multiplicity					14:25	High N-glycan multiplicity	0:25	High N-glycan multiplicity	0:25	High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.					
30311906	5	20	theme	Srd5a3	687:692	arg1	deletion					694:701	Srd5a3 deletion	687:701	Srd5a3 deletion	687:701	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	5	21	theme	motor	621:625	arg1	defects					640:646	motor coordination defects	621:646	motor coordination defects	621:646	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	0	22	theme	High	0:3	arg1	multiplicity					14:25	High N-glycan multiplicity	0:25	High N-glycan multiplicity	0:25	High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.					
30311906	5	23	theme	coordination	627:638	arg1	defects					640:646	motor coordination defects	621:646	motor coordination defects	621:646	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	0	24	theme	neuronal	43:50	arg1	adhesion					52:59	neuronal adhesion	43:59	neuronal adhesion	43:59	High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.					
30311906	1	25	theme	neuronal	206:213	arg1	migration					215:223	neuronal migration	206:223	neuronal migration	206:223	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	4	26	theme	N-glycosylation	589:603	arg1	initiation					575:584	the initiation	571:584	the initiation of N-glycosylation	571:603	We generated a cerebellum specific knockout mouse for Srd5a3, a gene involved in the initiation of N-glycosylation.					
30311906	8	27	theme	N-glycan	1201:1208	arg1	multiplicity					1210:1221	high N-glycan multiplicity	1196:1221	high N-glycan multiplicity	1196:1221	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	7	28	theme	neuron	1032:1037	arg1	adhesion					1039:1046	neuron adhesion	1032:1046	neuron adhesion	1032:1046	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	2	29	theme	neurological	333:344	arg1	symptoms					346:353	broad neurological symptoms	327:353	broad neurological symptoms identified in congenital disorders of glycosylation	327:405	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	8	30	theme	high	1196:1199	arg1	multiplicity					1210:1221	high N-glycan multiplicity	1196:1221	high N-glycan multiplicity	1196:1221	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	6	31	theme	N-glycosylation	962:976	arg1	level					978:982	N-glycosylation level	962:982	N-glycosylation level	962:982	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	2	32	theme	glycosylation	393:405	arg1	disorders					380:388	congenital disorders	369:388	congenital disorders of glycosylation	369:405	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	8	33	theme	neural	1237:1242	arg1	adhesion					1249:1256	fine-tuned neural cell adhesion	1226:1256	fine-tuned neural cell adhesion	1226:1256	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	1	34	theme	axon	226:229	arg1	guidance					231:238	axon guidance	226:238	axon guidance	226:238	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	4	35	theme	specific	516:523	arg1	mouse					534:538	a cerebellum specific knockout mouse	503:538	a cerebellum specific knockout mouse for Srd5a3, a gene involved in the initiation of N-glycosylation	503:603	We generated a cerebellum specific knockout mouse for Srd5a3, a gene involved in the initiation of N-glycosylation.					
30311906	7	36	theme	axon	1136:1139	arg1	guidance					1141:1148	axon guidance	1136:1148	axon guidance	1136:1148	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	8	37	attach	link	1191:1194	arg2	results					1183:1189	Our results	1179:1189	Our results	1179:1189	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	8	37	attach	link	1191:1194	arg1	adhesion					1249:1256	fine-tuned neural cell adhesion	1226:1256	fine-tuned neural cell adhesion	1226:1256	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	6	38	with	glycoproteins	868:880	arg1	multiplicity					902:913	high N-glycans multiplicity	887:913	high N-glycans multiplicity per protein	887:925	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	6	39	theme	high	887:890	arg1	multiplicity					902:913	high N-glycans multiplicity	887:913	high N-glycans multiplicity per protein	887:925	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	6	40	gly	glycoproteins	868:880	arg1	glycoproteins					868:880	glycoproteins	868:880	glycoproteins with high N-glycans multiplicity per protein	868:925	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	7	41	dep	critical	1019:1026	arg1	N-glycosylated					1059:1072	N-glycosylated	1059:1072	N-glycosylated	1059:1072	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	3	42	theme	molecular	443:451	arg1	mechanisms					453:462	the molecular mechanisms	439:462	the molecular mechanisms underlying these defects	439:487	However, little is known about the molecular mechanisms underlying these defects.					
30311906	7	43	theme	impaired	1087:1094	arg1	outgrowth					1122:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	8	44	theme	fine-tuned	1226:1235	arg1	adhesion					1249:1256	fine-tuned neural cell adhesion	1226:1256	fine-tuned neural cell adhesion	1226:1256	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	6	45	theme	Srd5a3	836:841	arg1	loss					843:846	Srd5a3 loss	836:846	Srd5a3 loss	836:846	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	1	46	theme	Proper	129:134	arg1	development					142:152	Proper brain development	129:152	Proper brain development	129:152	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	6	47	theme	glycoproteins	868:880	arg1	subset					858:863	a subset	856:863	a subset of glycoproteins with high N-glycans multiplicity per protein	856:925	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	6	48	theme	N-glycans	892:900	arg1	multiplicity					902:913	high N-glycans multiplicity	887:913	high N-glycans multiplicity per protein	887:925	Using proteomic approaches, we identified that Srd5a3 loss affects a subset of glycoproteins with high N-glycans multiplicity per protein and decreased protein abundance or N-glycosylation level.					
30311906	1	49	theme	brain	136:140	arg1	development					142:152	Proper brain development	129:152	Proper brain development	129:152	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	5	50	theme	ER	773:774	arg1	homeostasis					776:786	ER homeostasis	773:786	ER homeostasis	773:786	In addition to motor coordination defects and abnormal granule cell development, Srd5a3 deletion causes mild N-glycosylation impairment without significantly altering ER homeostasis.					
30311906	2	51	theme	N-glycosylation	279:293	arg1	pathway					295:301	the N-glycosylation pathway	275:301	the N-glycosylation pathway	275:301	Impairing the N-glycosylation pathway at early steps produces broad neurological symptoms identified in congenital disorders of glycosylation.					
30311906	7	52	theme	IgSF-CAM-mediated	1096:1112	arg1	outgrowth					1122:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	7	53	theme	adhesion	997:1004	arg1	proteins					1006:1013	IgSF-CAM adhesion proteins	988:1013	IgSF-CAM adhesion proteins	988:1013	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	1	54	theme	synaptic	244:251	arg1	physiology					253:262	synaptic physiology	244:262	synaptic physiology	244:262	Proper brain development relies highly on protein N-glycosylation to sustain neuronal migration, axon guidance and synaptic physiology.					
30311906	8	55	theme	mammalian	1265:1273	arg1	development					1281:1291	mammalian brain development	1265:1291	mammalian brain development	1265:1291	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30311906	7	56	theme	mutant	1160:1165	arg1	cerebellum					1167:1176	Srd5a3 mutant cerebellum	1153:1176	Srd5a3 mutant cerebellum	1153:1176	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	7	57	theme	neurite	1114:1120	arg1	outgrowth					1122:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	impaired IgSF-CAM-mediated neurite outgrowth	1087:1130	As IgSF-CAM adhesion proteins are critical for neuron adhesion and highly N-glycosylated, we observed impaired IgSF-CAM-mediated neurite outgrowth and axon guidance in Srd5a3 mutant cerebellum.					
30311906	8	58	theme	cell	1244:1247	arg1	adhesion					1249:1256	fine-tuned neural cell adhesion	1226:1256	fine-tuned neural cell adhesion	1226:1256	Our results link high N-glycan multiplicity to fine-tuned neural cell adhesion during mammalian brain development.					
30444974	3	0	theme	E.	395:396	arg1	ubiquitin					410:418	E. histolytica ubiquitin	395:418	E. histolytica ubiquitin	395:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	5	1	theme	mannose	948:954	arg1	structures					965:974	high mannose N-glycan structures	943:974	high mannose N-glycan structures	943:974	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	3	2	theme	histolytica	398:408	arg1	ubiquitin					410:418	E. histolytica ubiquitin	395:418	E. histolytica ubiquitin	395:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	3	from	ubiquitin	410:418	arg1	characterization					341:356	the partial characterization	329:356	the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis	329:681	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	4	theme	Gas	629:631	arg1	GC-MS					667:671	GC-MS	667:671	GC-MS	667:671	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	4	theme	Gas	629:631	arg1	Spectrometry					653:664	Gas Chromatography-Mass Spectrometry	629:664	Gas Chromatography-Mass Spectrometry (GC-MS)	629:672	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	8	5	theme	possible	1310:1317	arg1	structures					1341:1350	possible high mannose N-glycan structures	1310:1350	possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1310:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	0	6	from	Characterization	0:15	arg1	histolytica					50:60	Entamoeba histolytica	40:60	Entamoeba histolytica	40:60	Characterization of N-glycosylations in Entamoeba histolytica ubiquitin.					
30444974	8	7	theme	N-glycan	1332:1339	arg1	structures					1341:1350	possible high mannose N-glycan structures	1310:1350	possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1310:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	1	8	theme	Entamoeba	73:81	arg1	histolytica					83:93	Entamoeba histolytica	73:93	Entamoeba histolytica	73:93	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	3	9	from	characterization	341:356	arg1	ubiquitin					410:418	E. histolytica ubiquitin	395:418	E. histolytica ubiquitin	395:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	7	10	theme	ubiquitin	1081:1089	arg1	function					1091:1098	ubiquitin function	1081:1098	ubiquitin function	1081:1098	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	4	11	theme	glycan	875:880	arg1	profiles					882:889	whole parasitic glycan profiles	859:889	whole parasitic glycan profiles	859:889	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	8	12	theme	structures	1341:1350	arg1	set					1303:1305	a set	1301:1305	a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1301:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	8	12	theme	structures	1341:1350	arg1	structures					1341:1350	possible high mannose N-glycan structures	1310:1350	possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1310:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	3	13	theme	Fluorophore-Assisted	423:442	arg1	FACE					474:477	FACE	474:477	FACE	474:477	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	13	theme	Fluorophore-Assisted	423:442	arg1	Electrophoresis					457:471	Fluorophore-Assisted Carbohydrate Electrophoresis	423:471	Fluorophore-Assisted Carbohydrate Electrophoresis (FACE)	423:478	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	1	14	theme	ubiquitin-proteasome	146:165	arg1	systems					167:173	ubiquitin-proteasome systems	146:173	ubiquitin-proteasome systems important for numerous cellular processes	146:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	7	15	theme	ubiquitin	1246:1254	arg1	requirements					1267:1278	ubiquitin functional requirements	1246:1278	ubiquitin functional requirements	1246:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	5	16	theme	N-glycan	956:963	arg1	structures					965:974	high mannose N-glycan structures	943:974	high mannose N-glycan structures	943:974	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	4	17	theme	histolytica	793:803	arg1	ubiquitin					805:813	E. histolytica ubiquitin	790:813	E. histolytica ubiquitin	790:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	8	18	theme	biochemical	1394:1404	arg1	composition					1406:1416	the ubiquitin biochemical composition	1380:1416	the ubiquitin biochemical composition	1380:1416	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	3	19	theme	Carbohydrate	444:455	arg1	FACE					474:477	FACE	474:477	FACE	474:477	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	19	theme	Carbohydrate	444:455	arg1	Electrophoresis					457:471	Fluorophore-Assisted Carbohydrate Electrophoresis	423:471	Fluorophore-Assisted Carbohydrate Electrophoresis (FACE)	423:478	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	7	20	theme	functional	1256:1265	arg1	requirements					1267:1278	ubiquitin functional requirements	1246:1278	ubiquitin functional requirements	1246:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	5	21	theme	ubiquitin	929:937	arg1	profile					903:909	The glycan profile	892:909	The glycan profile of E. histolytica ubiquitin	892:937	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	6	22	link	N-linked	981:988	arg1	profile					997:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile showed fragments from Hex3HexNAc2 to Hex9HexNAc2.					
30444974	3	23	theme	Matrix-Assisted	544:558	arg1	MS					621:622	MALDI-TOF MS	611:622	MALDI-TOF MS	611:622	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	23	theme	Matrix-Assisted	544:558	arg1	Spectrometry					597:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry	544:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS)	544:623	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	5	24	theme	histolytica	917:927	arg1	ubiquitin					929:937	E. histolytica ubiquitin	914:937	E. histolytica ubiquitin	914:937	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	7	25	contain	carries	1159:1165	arg3	stages					1226:1231	different metabolic pathway stages	1198:1231	different metabolic pathway stages according to ubiquitin functional requirements	1198:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	7	25	contain	carries	1159:1165	arg1	sequon					1152:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	7	25	contain	carries	1159:1165	arg2	glycosylations					1179:1192	heterogenic glycosylations	1167:1192	heterogenic glycosylations	1167:1192	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	1	26	theme	extensive	106:114	arg1	distribution					130:141	an extensive intracellular distribution	103:141	an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes	103:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	4	27	theme	glycomics	768:776	arg1	analysis					778:785	the first structural glycomics analysis	747:785	the first structural glycomics analysis of E. histolytica ubiquitin	747:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	3	28	theme	Nanoelectrospray	481:496	arg1	NSI-MS					535:540	NSI-MS	535:540	NSI-MS	535:540	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	28	theme	Nanoelectrospray	481:496	arg1	Spectrometry					521:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry	481:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS)	481:541	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	7	29	theme	metabolic	1208:1216	arg1	stages					1226:1231	different metabolic pathway stages	1198:1231	different metabolic pathway stages according to ubiquitin functional requirements	1198:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	1	30	theme	intracellular	116:128	arg1	distribution					130:141	an extensive intracellular distribution	103:141	an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes	103:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	4	31	theme	E.	790:791	arg1	ubiquitin					805:813	E. histolytica ubiquitin	790:813	E. histolytica ubiquitin	790:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	8	32	theme	high	1319:1322	arg1	structures					1341:1350	possible high mannose N-glycan structures	1310:1350	possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1310:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	7	33	theme	pathway	1218:1224	arg1	stages					1226:1231	different metabolic pathway stages	1198:1231	different metabolic pathway stages according to ubiquitin functional requirements	1198:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	4	34	theme	parasitic	865:873	arg1	profiles					882:889	whole parasitic glycan profiles	859:889	whole parasitic glycan profiles	859:889	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	3	35	theme	Mass	592:595	arg1	MS					621:622	MALDI-TOF MS	611:622	MALDI-TOF MS	611:622	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	35	theme	Mass	592:595	arg1	Spectrometry					597:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry	544:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS)	544:623	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	0	36	theme	N-glycosylations	20:35	arg1	Characterization					0:15	Characterization	0:15	Characterization of N-glycosylations in Entamoeba histolytica	0:60	Characterization of N-glycosylations in Entamoeba histolytica ubiquitin.					
30444974	7	37	theme	heterogenic	1167:1177	arg1	glycosylations					1179:1192	heterogenic glycosylations	1167:1192	heterogenic glycosylations	1167:1192	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	7	38	theme	Asn-Asp-Ser	1140:1150	arg1	sequon					1152:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	4	39	theme	whole	859:863	arg1	profiles					882:889	whole parasitic glycan profiles	859:889	whole parasitic glycan profiles	859:889	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	5	40	theme	E.	914:915	arg1	ubiquitin					929:937	E. histolytica ubiquitin	914:937	E. histolytica ubiquitin	914:937	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	1	41	theme	numerous	189:196	arg1	processes					207:215	numerous cellular processes	189:215	numerous cellular processes	189:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	5	42	theme	glycan	896:901	arg1	profile					903:909	The glycan profile	892:909	The glycan profile of E. histolytica ubiquitin	892:937	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	7	43	theme	different	1198:1206	arg1	stages					1226:1231	different metabolic pathway stages	1198:1231	different metabolic pathway stages according to ubiquitin functional requirements	1198:1278	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	2	44	theme	glycosylation	227:239	arg1	studies					241:247	glycosylation studies	227:247	glycosylation studies of ubiquitin-proteasome components	227:282	However, glycosylation studies of ubiquitin-proteasome components have not yet been elucidated.					
30444974	3	45	theme	Laser-Desorption	560:575	arg1	MS					621:622	MALDI-TOF MS	611:622	MALDI-TOF MS	611:622	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	45	theme	Laser-Desorption	560:575	arg1	Spectrometry					597:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry	544:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS)	544:623	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	0	46	theme	Entamoeba	40:48	arg1	histolytica					50:60	Entamoeba histolytica	40:60	Entamoeba histolytica	40:60	Characterization of N-glycosylations in Entamoeba histolytica ubiquitin.					
30444974	8	47	theme	good	1441:1444	arg1	targets					1446:1452	good targets	1441:1452	good targets for anti-amoebic drugs	1441:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	3	48	theme	partial	333:339	arg1	characterization					341:356	the partial characterization	329:356	the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis	329:681	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	6	49	theme	N-linked	981:988	arg1	profile					997:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile showed fragments from Hex3HexNAc2 to Hex9HexNAc2.					
30444974	0	50	dep	ubiquitin	62:70	arg1	Characterization					0:15	Characterization	0:15	Characterization of N-glycosylations in Entamoeba histolytica	0:60	Characterization of N-glycosylations in Entamoeba histolytica ubiquitin.					
30444974	8	51	theme	anti-amoebic	1458:1469	arg1	drugs					1471:1475	anti-amoebic drugs	1458:1475	anti-amoebic drugs	1458:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	3	52	theme	time-of-flight	577:590	arg1	MS					621:622	MALDI-TOF MS	611:622	MALDI-TOF MS	611:622	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	52	theme	time-of-flight	577:590	arg1	Spectrometry					597:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry	544:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS)	544:623	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	53	theme	Ionization-Tandem	498:514	arg1	NSI-MS					535:540	NSI-MS	535:540	NSI-MS	535:540	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	53	theme	Ionization-Tandem	498:514	arg1	Spectrometry					521:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry	481:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS)	481:541	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	54	theme	profile	384:390	arg1	characterization					341:356	the partial characterization	329:356	the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis	329:681	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	55	dep	Electrophoresis	457:471	arg1	analysis					674:681	analysis	674:681	analysis	674:681	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	56	link	N-linked	361:368	arg1	glycosylation					370:382	N-linked glycosylation	361:382	N-linked glycosylation profile in E. histolytica ubiquitin	361:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	57	theme	Mass	516:519	arg1	NSI-MS					535:540	NSI-MS	535:540	NSI-MS	535:540	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	57	theme	Mass	516:519	arg1	Spectrometry					521:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry	481:532	Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS)	481:541	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	7	58	theme	ubiquitin	1130:1138	arg1	sequon					1152:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	1	59	theme	systems	167:173	arg1	distribution					130:141	an extensive intracellular distribution	103:141	an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes	103:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	7	60	theme	same	1125:1128	arg1	sequon					1152:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	the same ubiquitin Asn-Asp-Ser sequon	1121:1157	Based in our findings and ubiquitin function, we hypothesize that the same ubiquitin Asn-Asp-Ser sequon carries heterogenic glycosylations, at different metabolic pathway stages according to ubiquitin functional requirements.					
30444974	3	61	theme	N-linked	361:368	arg1	glycosylation					370:382	N-linked glycosylation	361:382	N-linked glycosylation profile in E. histolytica ubiquitin	361:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	2	62	theme	components	273:282	arg1	studies					241:247	glycosylation studies	227:247	glycosylation studies of ubiquitin-proteasome components	227:282	However, glycosylation studies of ubiquitin-proteasome components have not yet been elucidated.					
30444974	6	63	from	Hex3HexNAc2	1027:1037	arg1	fragments					1012:1020	fragments	1012:1020	fragments from Hex3HexNAc2 to Hex9HexNAc2	1012:1052	The N-linked glycan profile showed fragments from Hex3HexNAc2 to Hex9HexNAc2.					
30444974	4	64	theme	ubiquitin	805:813	arg1	analysis					778:785	the first structural glycomics analysis	747:785	the first structural glycomics analysis of E. histolytica ubiquitin	747:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	1	65	theme	important	175:183	arg1	systems					167:173	ubiquitin-proteasome systems	146:173	ubiquitin-proteasome systems important for numerous cellular processes	146:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
30444974	3	66	theme	glycosylation	370:382	arg1	profile					384:390	N-linked glycosylation profile	361:390	N-linked glycosylation profile in E. histolytica ubiquitin	361:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	2	67	theme	ubiquitin-proteasome	252:271	arg1	components					273:282	ubiquitin-proteasome components	252:282	ubiquitin-proteasome components	252:282	However, glycosylation studies of ubiquitin-proteasome components have not yet been elucidated.					
30444974	5	68	theme	high	943:946	arg1	structures					965:974	high mannose N-glycan structures	943:974	high mannose N-glycan structures	943:974	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	3	69	theme	MALDI-TOF	611:619	arg1	MS					621:622	MALDI-TOF MS	611:622	MALDI-TOF MS	611:622	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	69	theme	MALDI-TOF	611:619	arg1	Spectrometry					597:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry	544:608	Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS)	544:623	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	6	70	theme	glycan	990:995	arg1	profile					997:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile	977:1003	The N-linked glycan profile showed fragments from Hex3HexNAc2 to Hex9HexNAc2.					
30444974	4	71	theme	structural	757:766	arg1	analysis					778:785	the first structural glycomics analysis	747:785	the first structural glycomics analysis of E. histolytica ubiquitin	747:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	4	72	theme	first	751:755	arg1	analysis					778:785	the first structural glycomics analysis	747:785	the first structural glycomics analysis of E. histolytica ubiquitin	747:813	To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles.					
30444974	3	73	theme	Chromatography-Mass	633:651	arg1	GC-MS					667:671	GC-MS	667:671	GC-MS	667:671	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	3	73	theme	Chromatography-Mass	633:651	arg1	Spectrometry					653:664	Gas Chromatography-Mass Spectrometry	629:664	Gas Chromatography-Mass Spectrometry (GC-MS)	629:672	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	8	74	theme	ubiquitin	1384:1392	arg1	composition					1406:1416	the ubiquitin biochemical composition	1380:1416	the ubiquitin biochemical composition	1380:1416	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	3	75	from	profile	384:390	arg1	ubiquitin					410:418	E. histolytica ubiquitin	395:418	E. histolytica ubiquitin	395:418	Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.					
30444974	8	76	theme	mannose	1324:1330	arg1	structures					1341:1350	possible high mannose N-glycan structures	1310:1350	possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs	1310:1475	Finally, we propose a set of possible high mannose N-glycan structures that will help to elucidate the ubiquitin biochemical composition and may well represent good targets for anti-amoebic drugs.					
30444974	5	77	contain	has	939:941	arg1	profile					903:909	The glycan profile	892:909	The glycan profile of E. histolytica ubiquitin	892:937	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	5	77	contain	has	939:941	arg2	structures					965:974	high mannose N-glycan structures	943:974	high mannose N-glycan structures	943:974	The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures.					
30444974	1	78	theme	cellular	198:205	arg1	processes					207:215	numerous cellular processes	189:215	numerous cellular processes	189:215	Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes.					
31606074	3	0	theme	glycopeptide	230:241	arg1	level					243:247	the glycopeptide level	226:247	the glycopeptide level	226:247	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	5	1	theme	mass	657:660	arg1	spectrometry					662:673	mass spectrometry	657:673	mass spectrometry (LC-MS)	657:681	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	5	1	theme	mass	657:660	arg1	LC-MS					676:680	LC-MS	676:680	LC-MS	676:680	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	4	2	gly	heterogeneity	461:473	arg1	tools					511:515	enabling analytical tools	491:515	enabling analytical tools	491:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	3	theme	tools	511:515	arg1	heterogeneity					461:473	structural heterogeneity	450:473	structural heterogeneity	450:473	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	3	theme	tools	511:515	arg1	lack					483:486	the lack	479:486	the lack of enabling analytical tools	479:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	3	theme	tools	511:515	arg1	abundance					439:447	their low abundance	429:447	their low abundance	429:447	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	8	4	theme	neuropeptide	1034:1045	arg1	database					1047:1054	a complete neuropeptide database	1023:1054	a complete neuropeptide database	1023:1054	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	9	5	theme	biological	1194:1203	arg1	complexity					1205:1214	biological complexity	1194:1214	biological complexity rendered by glycosylation	1194:1240	Other strategies and future directions are discussed to provide readers with alternative approaches and further unravel biological complexity rendered by glycosylation.					
31606074	4	6	theme	analytical	500:509	arg1	tools					511:515	enabling analytical tools	491:515	enabling analytical tools	491:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	7	theme	low	435:437	arg1	abundance					439:447	their low abundance	429:447	their low abundance	429:447	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	6	8	theme	further	763:769	arg1	separation/cleanup					771:788	further separation/cleanup	763:788	further separation/cleanup	763:788	We first demonstrate how to extract neuropeptides from raw tissues and perform further separation/cleanup before MS analysis.					
31606074	4	9	theme	signaling	403:411	arg1	peptides					413:420	glycosylated endogenous signaling peptides	379:420	glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools	379:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	8	10	theme	glycosylated	975:986	arg1	neuropeptides					988:1000	glycosylated neuropeptides	975:1000	glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking	975:1071	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	9	11	theme	Other	1074:1078	arg1	strategies					1080:1089	Other strategies	1074:1089	Other strategies	1074:1089	Other strategies and future directions are discussed to provide readers with alternative approaches and further unravel biological complexity rendered by glycosylation.					
31606074	9	12	with	readers	1138:1144	arg1	approaches					1163:1172	alternative approaches	1151:1172	alternative approaches	1151:1172	Other strategies and future directions are discussed to provide readers with alternative approaches and further unravel biological complexity rendered by glycosylation.					
31606074	4	13	theme	endogenous	392:401	arg1	peptides					413:420	glycosylated endogenous signaling peptides	379:420	glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools	379:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	14	theme	due	422:424	arg1	peptides					413:420	glycosylated endogenous signaling peptides	379:420	glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools	379:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	1	15	theme	ubiquitous	95:104	arg1	PTMs					152:155	PTMs	152:155	PTMs	152:155	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	1	15	theme	ubiquitous	95:104	arg1	modifications					137:149	the most ubiquitous and complex post-translational modifications	86:149	the most ubiquitous and complex post-translational modifications (PTMs)	86:156	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	8	16	gly	glycosylated	975:986	arg1	neuropeptides					988:1000	glycosylated neuropeptides	975:1000	glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking	975:1071	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	5	17	gly	glycosylated	576:587	arg1	neuropeptides					589:601	glycosylated neuropeptides	576:601	glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS)	576:681	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	6	18	from	tissues	743:749	arg1	extract					712:718	extract	712:718	extract	712:718	We first demonstrate how to extract neuropeptides from raw tissues and perform further separation/cleanup before MS analysis.					
31606074	6	19	theme	raw	739:741	arg1	tissues					743:749	raw tissues	739:749	raw tissues	739:749	We first demonstrate how to extract neuropeptides from raw tissues and perform further separation/cleanup before MS analysis.					
31606074	9	20	theme	future	1095:1100	arg1	directions					1102:1111	future directions	1095:1111	future directions	1095:1111	Other strategies and future directions are discussed to provide readers with alternative approaches and further unravel biological complexity rendered by glycosylation.					
31606074	7	21	theme	neuropeptide	862:873	arg1	profiling					875:883	glycosylated neuropeptide profiling	849:883	glycosylated neuropeptide profiling	849:883	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	2	22	theme	various	185:191	arg1	processes					204:212	various biological processes	185:212	various biological processes	185:212	It plays pivotal roles in various biological processes.					
31606074	1	23	theme	complex	110:116	arg1	PTMs					152:155	PTMs	152:155	PTMs	152:155	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	1	23	theme	complex	110:116	arg1	modifications					137:149	the most ubiquitous and complex post-translational modifications	86:149	the most ubiquitous and complex post-translational modifications (PTMs)	86:156	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	0	24	theme	neuropeptides	47:59	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of glycosylated neuropeptides.					
31606074	0	24	theme	neuropeptides	47:59	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of glycosylated neuropeptides.					
31606074	4	25	gly	glycosylated	379:390	arg1	peptides					413:420	glycosylated endogenous signaling peptides	379:420	glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools	379:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	8	26	theme	complete	1025:1032	arg1	database					1047:1054	a complete neuropeptide database	1023:1054	a complete neuropeptide database	1023:1054	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	8	27	theme	data	949:952	arg1	analysis					954:961	data analysis	949:961	data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking	949:1071	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	0	28	theme	glycosylated	34:45	arg1	neuropeptides					47:59	glycosylated neuropeptides	34:59	glycosylated neuropeptides	34:59	Isolation and characterization of glycosylated neuropeptides.					
31606074	3	29	gly	glycopeptide	230:241	arg2	glycopeptide					230:241	the glycopeptide level	226:247	the glycopeptide level	226:247	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	4	30	theme	enabling	491:498	arg1	tools					511:515	enabling analytical tools	491:515	enabling analytical tools	491:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	5	31	theme	glycosylated	576:587	arg1	neuropeptides					589:601	glycosylated neuropeptides	576:601	glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS)	576:681	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	7	32	theme	glycosylated	849:860	arg1	profiling					875:883	glycosylated neuropeptide profiling	849:883	glycosylated neuropeptide profiling	849:883	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	3	33	theme	digested	320:327	arg1	glycoproteins					329:341	enzymatic digested glycoproteins	310:341	enzymatic digested glycoproteins	310:341	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	2	34	theme	pivotal	168:174	arg1	roles					176:180	pivotal roles	168:180	pivotal roles	168:180	It plays pivotal roles in various biological processes.					
31606074	1	35	theme	post-translational	118:135	arg1	PTMs					152:155	PTMs	152:155	PTMs	152:155	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	1	35	theme	post-translational	118:135	arg1	modifications					137:149	the most ubiquitous and complex post-translational modifications	86:149	the most ubiquitous and complex post-translational modifications (PTMs)	86:156	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	3	36	theme	downstream	279:288	arg1	work					290:293	a downstream work	277:293	a downstream work resulting from enzymatic digested glycoproteins	277:341	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	3	36	theme	downstream	279:288	arg1	Studies					215:221	Studies	215:221	Studies at the glycopeptide level	215:247	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	7	37	theme	MS	834:835	arg1	methods					837:843	hybrid MS methods	827:843	hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis	827:910	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	4	38	theme	structural	450:459	arg1	heterogeneity					461:473	structural heterogeneity	450:473	structural heterogeneity	450:473	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	4	39	theme	glycosylated	379:390	arg1	peptides					413:420	glycosylated endogenous signaling peptides	379:420	glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools	379:515	Less attention has been focused on glycosylated endogenous signaling peptides due to their low abundance, structural heterogeneity and the lack of enabling analytical tools.					
31606074	1	40	theme	modifications	137:149	arg1	PTMs					152:155	PTMs	152:155	PTMs	152:155	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	1	40	theme	modifications	137:149	arg1	one					79:81	one	79:81	one	79:81	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	1	40	theme	modifications	137:149	arg1	modifications					137:149	the most ubiquitous and complex post-translational modifications	86:149	the most ubiquitous and complex post-translational modifications (PTMs)	86:156	Glycosylation is one of the most ubiquitous and complex post-translational modifications (PTMs).					
31606074	3	41	from	level	243:247	arg1	work					290:293	a downstream work	277:293	a downstream work resulting from enzymatic digested glycoproteins	277:341	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	3	41	from	level	243:247	arg1	Studies					215:221	Studies	215:221	Studies at the glycopeptide level	215:247	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	5	42	theme	liquid	622:627	arg1	chromatography					629:642	nanoflow liquid chromatography	613:642	nanoflow liquid chromatography coupled with mass spectrometry (LC-MS)	613:681	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	0	43	gly	glycosylated	34:45	arg1	neuropeptides					47:59	glycosylated neuropeptides	34:59	glycosylated neuropeptides	34:59	Isolation and characterization of glycosylated neuropeptides.					
31606074	5	44	theme	nanoflow	613:620	arg1	chromatography					629:642	nanoflow liquid chromatography	613:642	nanoflow liquid chromatography coupled with mass spectrometry (LC-MS)	613:681	Here, protocols are presented to isolate and characterize glycosylated neuropeptides utilizing nanoflow liquid chromatography coupled with mass spectrometry (LC-MS).					
31606074	3	45	theme	enzymatic	310:318	arg1	glycoproteins					329:341	enzymatic digested glycoproteins	310:341	enzymatic digested glycoproteins	310:341	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	7	46	gly	glycosylated	849:860	arg1	profiling					875:883	glycosylated neuropeptide profiling	849:883	glycosylated neuropeptide profiling	849:883	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	7	47	theme	hybrid	827:832	arg1	methods					837:843	hybrid MS methods	827:843	hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis	827:910	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	3	48	gly	glycoproteins	329:341	arg1	glycoproteins					329:341	enzymatic digested glycoproteins	310:341	enzymatic digested glycoproteins	310:341	Studies at the glycopeptide level are typically considered as a downstream work resulting from enzymatic digested glycoproteins.					
31606074	2	49	theme	biological	193:202	arg1	processes					204:212	various biological processes	185:212	various biological processes	185:212	It plays pivotal roles in various biological processes.					
31606074	7	50	theme	site-specific	889:901	arg1	analysis					903:910	site-specific analysis	889:910	site-specific analysis	889:910	Then we describe hybrid MS methods for glycosylated neuropeptide profiling and site-specific analysis.					
31606074	9	51	theme	alternative	1151:1161	arg1	approaches					1163:1172	alternative approaches	1151:1172	alternative approaches	1151:1172	Other strategies and future directions are discussed to provide readers with alternative approaches and further unravel biological complexity rendered by glycosylation.					
31606074	8	52	from	neuropeptides	988:1000	arg1	crustaceans					1005:1015	crustaceans	1005:1015	crustaceans where a complete neuropeptide database is still lacking	1005:1071	We also include recommendations for data analysis to identify glycosylated neuropeptides in crustaceans where a complete neuropeptide database is still lacking.					
31606074	6	53	theme	MS	797:798	arg1	analysis					800:807	MS analysis	797:807	MS analysis	797:807	We first demonstrate how to extract neuropeptides from raw tissues and perform further separation/cleanup before MS analysis.					
31489526	6	0	theme	isoform	731:737	arg1	profiles					739:746	charge-related isoform profiles	716:746	charge-related isoform profiles	716:746	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	10	1	theme	glycans	1299:1305	arg1	abundance					1247:1255	an increased abundance	1234:1255	an increased abundance of more branched, higher molecular weight glycans in NUCP A2M	1234:1317	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	10	2	theme	NUCP	1310:1313	arg1	A2M					1315:1317	NUCP A2M	1310:1317	NUCP A2M	1310:1317	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	10	3	theme	branched	1265:1272	arg1	glycans					1299:1305	more branched, higher molecular weight glycans	1260:1305	more branched, higher molecular weight glycans	1260:1305	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	0	4	from	Variation	14:22	arg1	Features					41:48	Glycosylation Features	27:48	Glycosylation Features of Alpha-2-Macroglobulin	27:73	Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin.					
31489526	2	5	theme	Glycosylation	217:229	arg1	characteristics					231:245	Glycosylation characteristics	217:245	Glycosylation characteristics	217:245	Glycosylation characteristics can affect protein structure and function.					
31489526	10	6	dep	molecular	1282:1290	arg1	higher					1275:1280	higher	1275:1280	higher	1275:1280	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	3	7	theme	pooled	390:395	arg1	plasmas					397:403	adult pooled plasmas	384:403	adult pooled plasmas	384:403	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	7	8	theme	acid	1013:1016	arg1	loss					1018:1021	sialic acid loss	1006:1021	sialic acid loss in the NUCP A2M population	1006:1048	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	8	9	theme	NUCP	1101:1104	arg1	isoforms					1110:1117	NUCP A2M isoforms	1101:1117	NUCP A2M isoforms	1101:1117	2D electrophoresis indicated a lower pI range for NUCP A2M isoforms.					
31489526	7	10	theme	NUCP	1030:1033	arg1	population					1039:1048	the NUCP A2M population	1026:1048	the NUCP A2M population	1026:1048	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	5	11	used	used	583:586	arg2	affinity					535:542	affinity	535:542	affinity for Ricinus Communis Agglutinin I	535:576	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	5	11	used	used	583:586	arg2	treatment					520:528	Neuraminidase treatment	506:528	Neuraminidase treatment	506:528	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	9	12	theme	terminal	1174:1181	arg1	galactose					1183:1191	higher terminal galactose	1167:1191	higher terminal galactose quantities	1167:1202	In addition, NUCP A2M displayed a trend toward higher terminal galactose quantities than adult A2M.					
31489526	8	13	theme	2D	1051:1052	arg1	electrophoresis					1054:1068	2D electrophoresis	1051:1068	2D electrophoresis	1051:1068	2D electrophoresis indicated a lower pI range for NUCP A2M isoforms.					
31489526	7	14	theme	N-glycan	893:900	arg1	mass					902:905	total N-glycan mass	887:905	total N-glycan mass	887:905	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	1	15	theme	spectrum	128:135	arg1	Alpha-2-macroglobulin					76:96	Alpha-2-macroglobulin	76:96	Alpha-2-macroglobulin (A2M)	76:102	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	15	theme	spectrum	128:135	arg1	inhibitor					137:145	a glycosylated broad spectrum inhibitor	107:145	a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation	107:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	6	16	theme	carbohydrate	773:784	arg1	FACE					803:806	FACE	803:806	FACE	803:806	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	6	16	theme	carbohydrate	773:784	arg1	electrophoresis					786:800	fluorophore-assisted carbohydrate electrophoresis	752:800	fluorophore-assisted carbohydrate electrophoresis (FACE)	752:807	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	1	17	gly	glycosylated	109:120	arg1	Alpha-2-macroglobulin					76:96	Alpha-2-macroglobulin	76:96	Alpha-2-macroglobulin (A2M)	76:102	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	17	gly	glycosylated	109:120	arg1	inhibitor					137:145	a glycosylated broad spectrum inhibitor	107:145	a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation	107:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	7	18	from	difference	873:882	arg1	mass					902:905	total N-glycan mass	887:905	total N-glycan mass	887:905	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	4	19	theme	Peptide	406:412	arg1	F					428:428	Peptide N-Glycosidase F	406:428	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	11	20	theme	A2M	1390:1392	arg1	clearance					1409:1417	A2M receptor-based clearance	1390:1417	A2M receptor-based clearance	1390:1417	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	6	21	theme	fluorophore-assisted	752:771	arg1	FACE					803:806	FACE	803:806	FACE	803:806	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	6	21	theme	fluorophore-assisted	752:771	arg1	electrophoresis					786:800	fluorophore-assisted carbohydrate electrophoresis	752:800	fluorophore-assisted carbohydrate electrophoresis (FACE)	752:807	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	11	22	from	differences	1326:1336	arg1	branching					1348:1356	glycan branching	1341:1356	glycan branching	1341:1356	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	11	22	from	differences	1326:1336	arg1	residues					1370:1377	charged residues	1362:1377	charged residues	1362:1377	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	8	23	theme	lower	1082:1086	arg1	range					1091:1095	a lower pI range	1080:1095	a lower pI range for NUCP A2M isoforms	1080:1117	2D electrophoresis indicated a lower pI range for NUCP A2M isoforms.					
31489526	5	24	theme	acid	615:618	arg1	content					634:640	terminal sialic acid and galactose content	599:640	terminal sialic acid and galactose content	599:640	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	10	25	from	abundance	1247:1255	arg1	A2M					1315:1317	NUCP A2M	1310:1317	NUCP A2M	1310:1317	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	5	26	theme	Ricinus	548:554	arg1	Agglutinin					565:574	Ricinus Communis Agglutinin I	548:576	Ricinus Communis Agglutinin I	548:576	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	1	27	theme	blood	198:202	arg1	coagulation					204:214	blood coagulation	198:214	blood coagulation	198:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	0	28	theme	Age-Dependent	0:12	arg1	Variation					14:22	Age-Dependent Variation	0:22	Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin	0:73	Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin.					
31489526	4	29	theme	F	428:428	arg1	treatment					430:438	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	7	30	from	change	973:978	arg1	charge					983:988	charge	983:988	charge	983:988	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	8	31	theme	pI	1088:1089	arg1	range					1091:1095	a lower pI range	1080:1095	a lower pI range for NUCP A2M isoforms	1080:1117	2D electrophoresis indicated a lower pI range for NUCP A2M isoforms.					
31489526	0	32	theme	Glycosylation	27:39	arg1	Features					41:48	Glycosylation Features	27:48	Glycosylation Features of Alpha-2-Macroglobulin	27:73	Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin.					
31489526	7	33	theme	total	887:891	arg1	mass					902:905	total N-glycan mass	887:905	total N-glycan mass	887:905	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	4	34	theme	N-Glycosidase	414:426	arg1	F					428:428	Peptide N-Glycosidase F	406:428	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	1	35	theme	glycosylated	109:120	arg1	Alpha-2-macroglobulin					76:96	Alpha-2-macroglobulin	76:96	Alpha-2-macroglobulin (A2M)	76:102	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	35	theme	glycosylated	109:120	arg1	inhibitor					137:145	a glycosylated broad spectrum inhibitor	107:145	a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation	107:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	5	36	theme	Neuraminidase	506:518	arg1	treatment					520:528	Neuraminidase treatment	506:528	Neuraminidase treatment	506:528	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	1	37	theme	broad	122:126	arg1	Alpha-2-macroglobulin					76:96	Alpha-2-macroglobulin	76:96	Alpha-2-macroglobulin (A2M)	76:102	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	37	theme	broad	122:126	arg1	inhibitor					137:145	a glycosylated broad spectrum inhibitor	107:145	a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation	107:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	6	38	theme	charge-related	716:729	arg1	profiles					739:746	charge-related isoform profiles	716:746	charge-related isoform profiles	716:746	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	6	39	used	used	813:816	arg2	electrophoresis					786:800	fluorophore-assisted carbohydrate electrophoresis	752:800	fluorophore-assisted carbohydrate electrophoresis (FACE)	752:807	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	6	39	used	used	813:816	arg2	FACE					803:806	FACE	803:806	FACE	803:806	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	7	40	theme	significant	933:943	arg1	difference					945:954	a statistically significant difference	917:954	a statistically significant difference	917:954	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	3	41	theme	glycosylation	310:322	arg1	characteristics					324:338	glycosylation characteristics	310:338	glycosylation characteristics of A2M	310:345	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	4	42	theme	molecules	495:503	arg1	content					480:486	the total N-glycan content	461:486	the total N-glycan content of the molecules	461:503	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	11	43	theme	increased	1457:1465	arg1	concentration					1471:1483	the increased A2M concentration	1453:1483	the increased A2M concentration seen in NUCP, and newborns	1453:1510	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	6	44	theme	N-glycan	834:841	arg1	profiles					843:850	N-glycan profiles	834:850	N-glycan profiles	834:850	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	3	45	theme	adult	384:388	arg1	plasmas					397:403	adult pooled plasmas	384:403	adult pooled plasmas	384:403	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	11	46	theme	receptor-based	1394:1407	arg1	clearance					1409:1417	A2M receptor-based clearance	1390:1417	A2M receptor-based clearance	1390:1417	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	11	47	theme	A2M	1467:1469	arg1	concentration					1471:1483	the increased A2M concentration	1453:1483	the increased A2M concentration seen in NUCP, and newborns	1453:1510	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	3	48	theme	newborn	350:356	arg1	NUCP					374:377	NUCP	374:377	NUCP	374:377	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	3	48	theme	newborn	350:356	arg1	cord					368:371	newborn umbilical cord	350:371	newborn umbilical cord (NUCP)	350:378	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	2	49	theme	protein	258:264	arg1	structure					266:274	protein structure	258:274	protein structure	258:274	Glycosylation characteristics can affect protein structure and function.					
31489526	6	50	dep	Two-dimensional	657:671	arg1	2D					674:675	2D	674:675	2D	674:675	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	4	51	used	used	444:447	arg2	treatment					430:438	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment	406:438	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	0	52	theme	Alpha-2-Macroglobulin	53:73	arg1	Features					41:48	Glycosylation Features	27:48	Glycosylation Features of Alpha-2-Macroglobulin	27:73	Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin.					
31489526	10	53	dep	branched	1265:1272	arg1	molecular					1282:1290	molecular	1282:1290	molecular	1282:1290	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	3	54	theme	umbilical	358:366	arg1	NUCP					374:377	NUCP	374:377	NUCP	374:377	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	3	54	theme	umbilical	358:366	arg1	cord					368:371	newborn umbilical cord	350:371	newborn umbilical cord (NUCP)	350:378	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	7	55	theme	sialic	1006:1011	arg1	loss					1018:1021	sialic acid loss	1006:1021	sialic acid loss in the NUCP A2M population	1006:1048	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	9	56	theme	galactose	1183:1191	arg1	quantities					1193:1202	higher terminal galactose quantities	1167:1202	higher terminal galactose quantities	1167:1202	In addition, NUCP A2M displayed a trend toward higher terminal galactose quantities than adult A2M.					
31489526	8	57	theme	A2M	1106:1108	arg1	isoforms					1110:1117	NUCP A2M isoforms	1101:1117	NUCP A2M isoforms	1101:1117	2D electrophoresis indicated a lower pI range for NUCP A2M isoforms.					
31489526	10	58	theme	weight	1292:1297	arg1	glycans					1299:1305	more branched, higher molecular weight glycans	1260:1305	more branched, higher molecular weight glycans	1260:1305	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	5	59	theme	galactose	624:632	arg1	content					634:640	terminal sialic acid and galactose content	599:640	terminal sialic acid and galactose content	599:640	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	3	60	theme	A2M	343:345	arg1	characteristics					324:338	glycosylation characteristics	310:338	glycosylation characteristics of A2M	310:345	This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas.					
31489526	5	61	theme	sialic	608:613	arg1	acid					615:618	sialic acid	608:618	sialic acid	608:618	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	5	62	theme	terminal	599:606	arg1	content					634:640	terminal sialic acid and galactose content	599:640	terminal sialic acid and galactose content	599:640	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	10	63	theme	increased	1237:1245	arg1	abundance					1247:1255	an increased abundance	1234:1255	an increased abundance of more branched, higher molecular weight glycans in NUCP A2M	1234:1317	FACE revealed an increased abundance of more branched, higher molecular weight glycans in NUCP A2M.					
31489526	11	64	theme	glycan	1341:1346	arg1	branching					1348:1356	glycan branching	1341:1356	glycan branching	1341:1356	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	4	65	theme	N-glycan	471:478	arg1	content					480:486	the total N-glycan content	461:486	the total N-glycan content of the molecules	461:503	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	9	66	theme	higher	1167:1172	arg1	galactose					1183:1191	higher terminal galactose	1167:1191	higher terminal galactose quantities	1167:1202	In addition, NUCP A2M displayed a trend toward higher terminal galactose quantities than adult A2M.					
31489526	5	67	theme	Communis	556:563	arg1	Agglutinin					565:574	Ricinus Communis Agglutinin I	548:576	Ricinus Communis Agglutinin I	548:576	Neuraminidase treatment, and affinity for Ricinus Communis Agglutinin I were used to examine terminal sialic acid and galactose content, respectively.					
31489526	1	68	theme	numerous	150:157	arg1	proteases					159:167	numerous proteases	150:167	numerous proteases	150:167	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	68	theme	numerous	150:157	arg1	those					180:184	those	180:184	those	180:184	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	9	69	theme	NUCP	1133:1136	arg1	A2M					1138:1140	NUCP A2M	1133:1140	NUCP A2M	1133:1140	In addition, NUCP A2M displayed a trend toward higher terminal galactose quantities than adult A2M.					
31489526	4	70	theme	total	465:469	arg1	content					480:486	the total N-glycan content	461:486	the total N-glycan content of the molecules	461:503	Peptide N-Glycosidase F treatment was used to evaluate the total N-glycan content of the molecules.					
31489526	6	71	used	used	698:701	arg2	electrophoresis					678:692	Two-dimensional (2D) electrophoresis	657:692	Two-dimensional (2D) electrophoresis	657:692	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	9	72	theme	adult	1209:1213	arg1	A2M					1215:1217	adult A2M	1209:1217	adult A2M	1209:1217	In addition, NUCP A2M displayed a trend toward higher terminal galactose quantities than adult A2M.					
31489526	1	73	theme	proteases	159:167	arg1	Alpha-2-macroglobulin					76:96	Alpha-2-macroglobulin	76:96	Alpha-2-macroglobulin (A2M)	76:102	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	1	73	theme	proteases	159:167	arg1	inhibitor					137:145	a glycosylated broad spectrum inhibitor	107:145	a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation	107:214	Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation.					
31489526	11	74	theme	charged	1362:1368	arg1	residues					1370:1377	charged residues	1362:1377	charged residues	1362:1377	These differences in glycan branching and charged residues may impact A2M receptor-based clearance and thus could be responsible for the increased A2M concentration seen in NUCP, and newborns.					
31489526	6	75	theme	Two-dimensional	657:671	arg1	electrophoresis					678:692	Two-dimensional (2D) electrophoresis	657:692	Two-dimensional (2D) electrophoresis	657:692	Two-dimensional (2D) electrophoresis was used to determine charge-related isoform profiles and fluorophore-assisted carbohydrate electrophoresis (FACE) was used to characterize N-glycan profiles.					
31489526	7	76	theme	A2M	1035:1037	arg1	population					1039:1048	the NUCP A2M population	1026:1048	the NUCP A2M population	1026:1048	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31489526	7	77	from	loss	1018:1021	arg1	population					1039:1048	the NUCP A2M population	1026:1048	the NUCP A2M population	1026:1048	Results revealed no difference in total N-glycan mass, however, a statistically significant difference was shown in the change in charge associated with sialic acid loss in the NUCP A2M population.					
31838283	0	0	theme	mass	105:108	arg1	spectrometry					110:121	capillary electrophoresis mass spectrometry	79:121	capillary electrophoresis mass spectrometry	79:121	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	3	1	theme	possible	556:563	arg1	quantification					565:578	the possible quantification	552:578	the possible quantification of site occupancy	552:596	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	4	2	theme	CE-MS	649:653	arg1	profiling					655:663	CE-MS profiling	649:663	CE-MS profiling	649:663	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	0	3	theme	electrophoresis	89:103	arg1	spectrometry					110:121	capillary electrophoresis mass spectrometry	79:121	capillary electrophoresis mass spectrometry	79:121	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	5	4	from	impact	1020:1025	arg1	charge					1055:1060	the net charge	1047:1060	the net charge of the protein	1047:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	4	5	theme	terminal	748:755	arg1	α-galactose					757:767	terminal α-galactose	748:767	terminal α-galactose	748:767	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	5	6	theme	gradient	961:968	arg1	chromatography					986:999	pH gradient cation-exchange chromatography	958:999	pH gradient cation-exchange chromatography	958:999	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	1	7	gly	glycosylation	138:150	arg1	study					191:195	this study	186:195	this study	186:195	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	1	7	gly	glycosylation	138:150	arg1	Cetuximab					155:163	Cetuximab	155:163	Cetuximab	155:163	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	2	8	theme	digested	323:330	arg1	Cetuximab					332:340	IdeS digested Cetuximab	318:340	IdeS digested Cetuximab with subsequent reduction	318:366	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	1	9	theme	multiple	203:210	arg1	methods					226:232	multiple fractionation methods	203:232	multiple fractionation methods	203:232	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	5	10	theme	charge	903:908	arg1	variants					910:917	different charge variants	893:917	different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein	893:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	0	11	theme	multiple	44:51	arg1	methods					67:73	multiple fractionation methods	44:73	multiple fractionation methods	44:73	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	2	12	dep	fragments	436:444	arg1	Lc					451:452	Lc	451:452	Lc	451:452	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	2	12	dep	fragments	436:444	arg1	Fd					447:448	Fd	447:448	Fd	447:448	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	2	12	dep	fragments	436:444	arg1	fragments					436:444	3 fragments	434:444	3 fragments; Fd, Lc and Fc/2	434:461	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	2	12	dep	fragments	436:444	arg1	Fc/2					458:461	Fc/2	458:461	Fc/2	458:461	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	1	13	theme	fractionation	212:224	arg1	methods					226:232	multiple fractionation methods	203:232	multiple fractionation methods	203:232	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	5	14	theme	Glycosylation	848:860	arg1	analysis					862:869	Glycosylation analysis	848:869	Glycosylation analysis	848:869	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	4	15	theme	Fd	835:836	arg1	fragment					838:845	the Fd fragment	831:845	the Fd fragment	831:845	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	5	16	theme	cation-exchange	970:984	arg1	chromatography					986:999	pH gradient cation-exchange chromatography	958:999	pH gradient cation-exchange chromatography	958:999	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	0	17	theme	N-Glycosylation	22:36	arg1	Analysis					0:7	Analysis	0:7	Analysis of cetuximab N-Glycosylation	0:36	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	4	18	theme	glycan	613:618	arg1	structures					620:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	5	19	theme	net	1051:1053	arg1	charge					1055:1060	the net charge	1047:1060	the net charge of the protein	1047:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	1	20	theme	Site-specific	124:136	arg1	glycosylation					138:150	Site-specific glycosylation	124:150	Site-specific glycosylation of Cetuximab	124:163	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	1	21	theme	capillary	238:246	arg1	electrophoresis					248:262	capillary electrophoresis	238:262	capillary electrophoresis coupled to mass spectrometry (CE-MS)	238:299	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	0	22	theme	cetuximab	12:20	arg1	N-Glycosylation					22:36	cetuximab N-Glycosylation	12:36	cetuximab N-Glycosylation	12:36	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	3	23	theme	occupancy	588:596	arg1	quantification					565:578	the possible quantification	552:578	the possible quantification of site occupancy	552:596	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	2	24	theme	subsequent	347:356	arg1	reduction					358:366	subsequent reduction	347:366	subsequent reduction	347:366	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	3	25	theme	18O	523:525	arg1	water					536:540	18O enriched water	523:540	18O enriched water providing the possible quantification of site occupancy	523:596	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	3	26	theme	Glycan	464:469	arg1	release					471:477	Glycan release	464:477	Glycan release of the different fragments	464:504	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	3	27	theme	different	486:494	arg1	fragments					496:504	the different fragments	482:504	the different fragments	482:504	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	5	28	theme	Cetuximab	922:930	arg1	variants					910:917	different charge variants	893:917	different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein	893:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	4	29	theme	potential	722:730	arg1	structures					732:741	potential structures	722:741	potential structures	722:741	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	5	30	theme	different	893:901	arg1	variants					910:917	different charge variants	893:917	different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein	893:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	5	31	theme	glycosylation	1030:1042	arg1	impact					1020:1025	the impact	1016:1025	the impact of glycosylation on the net charge of the protein	1016:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	0	32	theme	fractionation	53:65	arg1	methods					67:73	multiple fractionation methods	44:73	multiple fractionation methods	44:73	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	4	33	theme	labelled	604:611	arg1	structures					620:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	4	34	with	combination	668:678	arg1	sequencing					700:709	exoglycosidase sequencing	685:709	exoglycosidase sequencing	685:709	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	1	35	theme	Cetuximab	155:163	arg1	glycosylation					138:150	Site-specific glycosylation	124:150	Site-specific glycosylation of Cetuximab	124:163	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	4	36	theme	2-AA	599:602	arg1	structures					620:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures	599:629	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	2	37	theme	reversed-phase	391:404	arg1	chromatography					406:419	reversed-phase chromatography	391:419	reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2	391:461	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	1	38	theme	mass	275:278	arg1	CE-MS					294:298	CE-MS	294:298	CE-MS	294:298	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	1	38	theme	mass	275:278	arg1	spectrometry					280:291	mass spectrometry	275:291	mass spectrometry (CE-MS)	275:299	Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics.					
31838283	2	39	theme	IdeS	318:321	arg1	Cetuximab					332:340	IdeS digested Cetuximab	318:340	IdeS digested Cetuximab with subsequent reduction	318:366	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
31838283	4	40	theme	N-glycolyl-neuraminic	773:793	arg1	NGNA					801:804	NGNA	801:804	NGNA	801:804	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	4	40	theme	N-glycolyl-neuraminic	773:793	arg1	acid					795:798	N-glycolyl-neuraminic acid	773:798	N-glycolyl-neuraminic acid (NGNA)	773:805	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	3	41	theme	enriched	527:534	arg1	water					536:540	18O enriched water	523:540	18O enriched water providing the possible quantification of site occupancy	523:596	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	0	42	theme	capillary	79:87	arg1	electrophoresis					89:103	capillary electrophoresis	79:103	capillary electrophoresis mass spectrometry	79:121	Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry.					
31838283	5	43	theme	pH	958:959	arg1	chromatography					986:999	pH gradient cation-exchange chromatography	958:999	pH gradient cation-exchange chromatography	958:999	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	5	44	theme	protein	1069:1075	arg1	charge					1055:1060	the net charge	1047:1060	the net charge of the protein	1047:1075	Glycosylation analysis was also performed on different charge variants of Cetuximab that were separated using pH gradient cation-exchange chromatography to investigate the impact of glycosylation on the net charge of the protein.					
31838283	3	45	theme	fragments	496:504	arg1	release					471:477	Glycan release	464:477	Glycan release of the different fragments	464:504	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	4	46	theme	exoglycosidase	685:698	arg1	sequencing					700:709	exoglycosidase sequencing	685:709	exoglycosidase sequencing	685:709	2-AA labelled glycan structures were annotated by CE-MS profiling in combination with exoglycosidase sequencing, revealing potential structures with terminal α-galactose and N-glycolyl-neuraminic acid (NGNA) mainly originating from the Fd fragment.					
31838283	3	47	theme	site	583:586	arg1	occupancy					588:596	site occupancy	583:596	site occupancy	583:596	Glycan release of the different fragments was performed in 18O enriched water providing the possible quantification of site occupancy.					
31838283	2	48	with	Cetuximab	332:340	arg1	reduction					358:366	subsequent reduction	347:366	subsequent reduction	347:366	IdeS digested Cetuximab with subsequent reduction was fractionated using reversed-phase chromatography resulting in 3 fragments; Fd, Lc and Fc/2.					
30102512	2	0	theme	monosaccharides	360:374	arg1	composition					345:355	composition	345:355	composition	345:355	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	2	0	theme	monosaccharides	360:374	arg1	structure					331:339	chemical structure	322:339	chemical structure	322:339	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	7	1	dep	galactosylation	1573:1587	arg1	i.e.					1567:1570	i.e.	1567:1570	i.e.	1567:1570	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	2	2	from	structure	331:339	arg1	glycans					282:288	The mAb glycans	274:288	The mAb glycans	274:288	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	2	2	from	structure	331:339	arg1	heterogeneous					305:317	heterogeneous	305:317	heterogeneous	305:317	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	8	3	theme	orthogonal	1753:1762	arg1	measurement					1764:1774	a facile orthogonal measurement	1744:1774	a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination	1744:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	7	4	theme	mAb	1543:1545	arg1	attributes					1555:1564	clinically important mAb quality attributes	1522:1564	clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%))	1522:1619	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	2	5	from	composition	345:355	arg1	glycans					282:288	The mAb glycans	274:288	The mAb glycans	274:288	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	2	5	from	composition	345:355	arg1	heterogeneous					305:317	heterogeneous	305:317	heterogeneous	305:317	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	6	6	theme	unambiguous	1233:1243	arg1	determination					1245:1257	unambiguous determination	1233:1257	unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide	1233:1363	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	8	7	from	content	1850:1856	arg1	determination					1878:1890	glycan structure determination	1861:1890	glycan structure determination	1861:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	8	theme	configuration	1328:1340	arg1	determination					1245:1257	unambiguous determination	1233:1257	unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide	1233:1363	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	9	theme	chemical	560:567	arg1	labeling					569:576	chemical labeling	560:576	chemical labeling of the glycans	560:591	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	9	theme	chemical	560:567	arg1	digestion					538:546	extensive digestion	528:546	extensive digestion of the mAb	528:557	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	4	10	theme	products	903:910	arg1	quality					887:893	the quality	883:893	the quality of drug products	883:910	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	3	11	theme	column	594:599	arg1	separation					601:610	column separation	594:610	column separation	594:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	11	theme	column	594:599	arg1	digestion					538:546	extensive digestion	528:546	extensive digestion of the mAb	528:557	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	8	12	theme	glycosylation	1784:1796	arg1	characterization					1798:1813	mAb glycosylation characterization	1780:1813	mAb glycosylation characterization	1780:1813	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	7	13	dep	two-dimensional	1462:1476	arg1	2D					1479:1480	2D	1479:1480	2D	1479:1480	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	7	14	theme	peaks	1453:1457	arg1	analysis					1425:1432	The analysis	1421:1432	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra	1421:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	15	theme	standard	1203:1210	arg1	glycans					1212:1218	commercial standard glycans	1192:1218	commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules	1192:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	0	16	theme	Magnetic	130:137	arg1	Resonance					139:147	Middle-Down Nuclear Magnetic Resonance	110:147	Middle-Down Nuclear Magnetic Resonance	110:147	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	3	17	theme	glycans	585:591	arg1	labeling					569:576	chemical labeling	560:576	chemical labeling of the glycans	560:591	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	17	theme	glycans	585:591	arg1	digestion					538:546	extensive digestion	528:546	extensive digestion of the mAb	528:557	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	1	18	theme	quality	210:216	arg1	Glycosylation					150:162	Glycosylation	150:162	Glycosylation of monoclonal antibodies (mAbs)	150:194	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	1	18	theme	quality	210:216	arg1	attribute					218:226	a critical quality attribute	199:226	a critical quality attribute that can impact mAb drug efficacy and safety	199:271	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	7	19	theme	glycan-mapping	1674:1687	arg1	method					1689:1694	a standard glycan-mapping method	1663:1694	a standard glycan-mapping method	1663:1694	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	8	20	theme	chemical	1829:1836	arg1	content					1850:1856	improved chemical information content	1820:1856	improved chemical information content on glycan structure determination	1820:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	21	theme	linkage	1297:1303	arg1	position					1305:1312	glycosidic linkage position	1286:1312	glycosidic linkage position	1286:1312	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	5	22	theme	glycan	1081:1086	arg1	moieties					1088:1095	the glycan moieties	1077:1095	the glycan moieties	1077:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	6	23	theme	major	1372:1376	arg1	scaffolds					1387:1395	the major N-glycan scaffolds	1368:1395	the major N-glycan scaffolds found in mAb molecules	1368:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	24	theme	glycans	648:654	arg1	identity					636:643	the chemical identity	623:643	the chemical identity of glycans	623:654	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	8	25	theme	glycan	1861:1866	arg1	determination					1878:1890	glycan structure determination	1861:1890	glycan structure determination	1861:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	26	theme	chemical	1266:1273	arg1	structure					1275:1283	the chemical structure	1262:1283	the chemical structure	1262:1283	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	7	27	with	consistent	1622:1631	arg1	results					1649:1655	literature results	1638:1655	literature results using a standard glycan-mapping method	1638:1694	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	5	28	theme	middle-down	922:932	arg1	approach					981:988	a proof-of-concept approach	962:988	a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties	962:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	5	28	theme	middle-down	922:932	arg1	method					939:944	a "middle-down" NMR method	919:944	a "middle-down" NMR method	919:944	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	2	29	from	heterogeneous	305:317	arg1	composition					345:355	composition	345:355	composition	345:355	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	2	29	from	heterogeneous	305:317	arg1	structure					331:339	chemical structure	322:339	chemical structure	322:339	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	5	30	gly	glycosylation	1021:1033	arg1	drugs					1051:1055	marketed mAb drugs	1038:1055	marketed mAb drugs	1038:1055	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	4	31	theme	chemical	736:743	arg1	similarity					755:764	chemical structure similarity	736:764	chemical structure similarity	736:764	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	0	32	theme	Therapeutic	73:83	arg1	Antibodies					96:105	Therapeutic Monoclonal Antibodies	73:105	Therapeutic Monoclonal Antibodies	73:105	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	5	33	theme	NMR	935:937	arg1	approach					981:988	a proof-of-concept approach	962:988	a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties	962:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	5	33	theme	NMR	935:937	arg1	method					939:944	a "middle-down" NMR method	919:944	a "middle-down" NMR method	919:944	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	7	34	theme	glycan	1437:1442	arg1	peaks					1453:1457	glycan anomeric peaks	1437:1457	glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra	1437:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	4	35	theme	analytical	812:821	arg1	methods					823:829	orthogonal analytical methods	801:829	orthogonal analytical methods for measuring glycan chemistry	801:860	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	7	36	theme	Fuc	1614:1616	arg1	%					1617:1617	Fuc%	1614:1617	Fuc%	1614:1617	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	7	36	theme	Fuc	1614:1616	arg1	fucosylation					1600:1611	fucosylation	1600:1611	fucosylation (Fuc%)	1600:1618	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	0	37	theme	Middle-Down	110:120	arg1	Resonance					139:147	Middle-Down Nuclear Magnetic Resonance	110:147	Middle-Down Nuclear Magnetic Resonance	110:147	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	8	38	theme	standard	1924:1931	arg1	approaches					1933:1942	standard approaches	1924:1942	standard approaches	1924:1942	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	39	theme	chemical	1121:1128	arg1	assignments					1136:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	40	theme	mass-spectrometry	409:425	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	4	41	theme	mAb	790:792	arg1	drugs					794:798	mAb drugs	790:798	mAb drugs	790:798	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	0	42	theme	Chemical	0:7	arg1	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	5	43	theme	proof-of-concept	964:979	arg1	approach					981:988	a proof-of-concept approach	962:988	a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties	962:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	5	43	theme	proof-of-concept	964:979	arg1	method					939:944	a "middle-down" NMR method	919:944	a "middle-down" NMR method	919:944	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	6	44	theme	glycan	1107:1112	arg1	assignments					1136:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	45	theme	fluorescence	393:404	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	7	46	theme	NMR	1490:1492	arg1	spectra					1494:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	3	47	theme	detection	432:440	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	0	48	attach	Linked	63:68	arg2	Composition					23:33	Composition	23:33	Composition	23:33	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	0	48	attach	Linked	63:68	arg1	Antibodies					96:105	Therapeutic Monoclonal Antibodies	73:105	Therapeutic Monoclonal Antibodies	73:105	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	0	48	attach	Linked	63:68	arg2	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	3	49	theme	MS	428:429	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	0	50	theme	Glycans	44:50	arg1	Composition					23:33	Composition	23:33	Composition	23:33	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	0	50	theme	Glycans	44:50	arg1	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	4	51	theme	glycan	845:850	arg1	chemistry					852:860	glycan chemistry	845:860	glycan chemistry	845:860	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	3	52	theme	glycan	509:514	arg1	cleavage					516:523	glycan cleavage	509:523	glycan cleavage	509:523	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	5	53	theme	marketed	1038:1045	arg1	drugs					1051:1055	marketed mAb drugs	1038:1055	marketed mAb drugs	1038:1055	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	6	54	theme	13C	1165:1167	arg1	abundance					1177:1185	13C natural abundance	1165:1185	13C natural abundance	1165:1185	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	55	theme	molecular	694:702	arg1	values					711:716	molecular weight values	694:716	molecular weight values	694:716	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	1	56	theme	mAb	244:246	arg1	efficacy					253:260	mAb drug efficacy	244:260	mAb drug efficacy	244:260	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	5	57	theme	drugs	1051:1055	arg1	glycosylation					1021:1033	the domain-specific glycosylation	1001:1033	the domain-specific glycosylation of marketed mAb drugs	1001:1055	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	8	58	with	quantification	1896:1909	arg1	content					1850:1856	improved chemical information content	1820:1856	improved chemical information content on glycan structure determination	1820:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	3	59	theme	digestion	538:546	arg1	steps					500:504	multiple steps	491:504	multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation	491:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	8	60	theme	NMR	1724:1726	arg1	method					1728:1733	the middle-down NMR method	1708:1733	the middle-down NMR method	1708:1733	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	7	61	theme	quality	1547:1553	arg1	attributes					1555:1564	clinically important mAb quality attributes	1522:1564	clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%))	1522:1619	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	62	theme	monosaccharide	1350:1363	arg1	structure					1275:1283	the chemical structure	1262:1283	the chemical structure	1262:1283	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	62	theme	monosaccharide	1350:1363	arg1	configuration					1328:1340	anomeric configuration	1319:1340	anomeric configuration	1319:1340	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	62	theme	monosaccharide	1350:1363	arg1	position					1305:1312	glycosidic linkage position	1286:1312	glycosidic linkage position	1286:1312	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	7	63	theme	important	1533:1541	arg1	attributes					1555:1564	clinically important mAb quality attributes	1522:1564	clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%))	1522:1619	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	5	64	theme	"	933:933	arg1	approach					981:988	a proof-of-concept approach	962:988	a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties	962:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	5	64	theme	"	933:933	arg1	method					939:944	a "middle-down" NMR method	919:944	a "middle-down" NMR method	919:944	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	2	65	theme	mAb	278:280	arg1	glycans					282:288	The mAb glycans	274:288	The mAb glycans	274:288	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	2	65	theme	mAb	278:280	arg1	heterogeneous					305:317	heterogeneous	305:317	heterogeneous	305:317	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	8	66	theme	facile	1746:1751	arg1	measurement					1764:1774	a facile orthogonal measurement	1744:1774	a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination	1744:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	67	theme	anomeric	1319:1326	arg1	configuration					1328:1340	anomeric configuration	1319:1340	anomeric configuration	1319:1340	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	2	68	theme	chemical	322:329	arg1	structure					331:339	chemical structure	322:339	chemical structure	322:339	The mAb glycans are inherently heterogeneous in chemical structure and composition of monosaccharides.					
30102512	4	69	theme	drug	898:901	arg1	products					903:910	drug products	898:910	drug products	898:910	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	7	70	theme	1H-13C	1483:1488	arg1	spectra					1494:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	7	71	theme	anomeric	1444:1451	arg1	peaks					1453:1457	glycan anomeric peaks	1437:1457	glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra	1437:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	8	72	theme	mAb	1780:1782	arg1	characterization					1798:1813	mAb glycosylation characterization	1780:1813	mAb glycosylation characterization	1780:1813	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	1	73	theme	critical	201:208	arg1	Glycosylation					150:162	Glycosylation	150:162	Glycosylation of monoclonal antibodies (mAbs)	150:194	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	1	73	theme	critical	201:208	arg1	attribute					218:226	a critical quality attribute	199:226	a critical quality attribute that can impact mAb drug efficacy and safety	199:271	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	7	74	theme	standard	1665:1672	arg1	method					1689:1694	a standard glycan-mapping method	1663:1694	a standard glycan-mapping method	1663:1694	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	75	theme	commercial	1192:1201	arg1	glycans					1212:1218	commercial standard glycans	1192:1218	commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules	1192:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	1	76	gly	Glycosylation	150:162	arg1	mAbs					190:193	mAbs	190:193	mAbs	190:193	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	1	76	gly	Glycosylation	150:162	arg1	antibodies					178:187	monoclonal antibodies	167:187	monoclonal antibodies (mAbs)	167:194	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	6	77	theme	position	1305:1312	arg1	determination					1245:1257	unambiguous determination	1233:1257	unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide	1233:1363	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	8	78	theme	improved	1820:1827	arg1	content					1850:1856	improved chemical information content	1820:1856	improved chemical information content on glycan structure determination	1820:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	7	79	theme	literature	1638:1647	arg1	results					1649:1655	literature results	1638:1655	literature results using a standard glycan-mapping method	1638:1694	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	80	theme	glycosidic	1286:1295	arg1	position					1305:1312	glycosidic linkage position	1286:1312	glycosidic linkage position	1286:1312	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	4	81	theme	structure	745:753	arg1	similarity					755:764	chemical structure similarity	736:764	chemical structure similarity	736:764	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	6	82	theme	N-glycan	1378:1385	arg1	scaffolds					1387:1395	the major N-glycan scaffolds	1368:1395	the major N-glycan scaffolds found in mAb molecules	1368:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	5	83	theme	moieties	1088:1095	arg1	cleavage					1065:1072	cleavage	1065:1072	cleavage of the glycan moieties	1065:1095	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	8	84	theme	information	1838:1848	arg1	content					1850:1856	improved chemical information content	1820:1856	improved chemical information content on glycan structure determination	1820:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	6	85	located	found	1397:1401	arg1	molecules					1410:1418	mAb molecules	1406:1418	mAb molecules	1406:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	85	located	found	1397:1401	arg2	scaffolds					1387:1395	the major N-glycan scaffolds	1368:1395	the major N-glycan scaffolds found in mAb molecules	1368:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	86	theme	structure	1275:1283	arg1	determination					1245:1257	unambiguous determination	1233:1257	unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide	1233:1363	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	87	theme	chemical	627:634	arg1	identity					636:643	the chemical identity	623:643	the chemical identity of glycans	623:654	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	0	88	theme	Monoclonal	85:94	arg1	Antibodies					96:105	Therapeutic Monoclonal Antibodies	73:105	Therapeutic Monoclonal Antibodies	73:105	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	8	89	theme	structure	1868:1876	arg1	determination					1878:1890	glycan structure determination	1861:1890	glycan structure determination	1861:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	1	90	theme	antibodies	178:187	arg1	Glycosylation					150:162	Glycosylation	150:162	Glycosylation of monoclonal antibodies (mAbs)	150:194	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	1	90	theme	antibodies	178:187	arg1	attribute					218:226	a critical quality attribute	199:226	a critical quality attribute that can impact mAb drug efficacy and safety	199:271	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	7	91	from	spectra	1494:1500	arg1	analysis					1425:1432	The analysis	1421:1432	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra	1421:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	8	92	with	measurement	1764:1774	arg1	content					1850:1856	improved chemical information content	1820:1856	improved chemical information content on glycan structure determination	1820:1890	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	3	93	theme	established	381:391	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	4	94	theme	orthogonal	801:810	arg1	methods					823:829	orthogonal analytical methods	801:829	orthogonal analytical methods for measuring glycan chemistry	801:860	In demonstrating chemical structure similarity and comparability among mAb drugs, orthogonal analytical methods for measuring glycan chemistry are needed to ensure the quality of drug products.					
30102512	0	95	theme	Nuclear	122:128	arg1	Resonance					139:147	Middle-Down Nuclear Magnetic Resonance	110:147	Middle-Down Nuclear Magnetic Resonance	110:147	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	5	96	theme	domain-specific	1005:1019	arg1	glycosylation					1021:1033	the domain-specific glycosylation	1001:1033	the domain-specific glycosylation of marketed mAb drugs	1001:1055	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	7	97	from	analysis	1425:1432	arg1	spectra					1494:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	98	theme	shift	1130:1134	arg1	assignments					1136:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	99	theme	1H/13C	1114:1119	arg1	assignments					1136:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	6	100	theme	mAb	1406:1408	arg1	molecules					1410:1418	mAb molecules	1406:1418	mAb molecules	1406:1418	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	101	theme	glycosylation	454:466	arg1	evaluation					468:477	glycosylation evaluation	454:477	glycosylation evaluation	454:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	7	102	from	peaks	1453:1457	arg1	spectra					1494:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	103	theme	Complete	1098:1105	arg1	assignments					1136:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments	1098:1146	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	104	theme	or	406:407	arg1	methods					442:448	The established fluorescence or mass-spectrometry (MS) detection methods	377:448	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation	377:477	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	105	theme	retention	675:683	arg1	time					685:688	retention time	675:688	retention time	675:688	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	0	106	theme	Major	38:42	arg1	Glycans					44:50	Major Glycans	38:50	Major Glycans	38:50	Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-Down Nuclear Magnetic Resonance.					
30102512	3	107	theme	multiple	491:498	arg1	steps					500:504	multiple steps	491:504	multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation	491:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	108	theme	weight	704:709	arg1	values					711:716	molecular weight values	694:716	molecular weight values	694:716	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	1	109	theme	monoclonal	167:176	arg1	mAbs					190:193	mAbs	190:193	mAbs	190:193	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	1	109	theme	monoclonal	167:176	arg1	antibodies					178:187	monoclonal antibodies	167:187	monoclonal antibodies (mAbs)	167:194	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
30102512	3	110	theme	extensive	528:536	arg1	labeling					569:576	chemical labeling	560:576	chemical labeling of the glycans	560:591	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	110	theme	extensive	528:536	arg1	separation					601:610	column separation	594:610	column separation	594:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	110	theme	extensive	528:536	arg1	digestion					538:546	extensive digestion	528:546	extensive digestion of the mAb	528:557	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	7	111	theme	Gal	1590:1592	arg1	galactosylation					1573:1587	galactosylation	1573:1587	galactosylation (Gal%)	1573:1594	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	7	111	theme	Gal	1590:1592	arg1	%					1593:1593	Gal%	1590:1593	Gal%	1590:1593	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	7	112	theme	two-dimensional	1462:1476	arg1	spectra					1494:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	two-dimensional (2D) 1H-13C NMR spectra	1462:1500	The analysis of glycan anomeric peaks in two-dimensional (2D) 1H-13C NMR spectra yielded metrics for clinically important mAb quality attributes (i.e., galactosylation (Gal%) and fucosylation (Fuc%)), consistent with literature results using a standard glycan-mapping method.					
30102512	6	113	theme	natural	1169:1175	arg1	abundance					1177:1185	13C natural abundance	1165:1185	13C natural abundance	1165:1185	Complete glycan 1H/13C chemical shift assignments were obtained at 13C natural abundance from commercial standard glycans that allowed unambiguous determination of the chemical structure, glycosidic linkage position, and anomeric configuration of each monosaccharide in the major N-glycan scaffolds found in mAb molecules.					
30102512	3	114	theme	cleavage	516:523	arg1	steps					500:504	multiple steps	491:504	multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation	491:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	8	115	theme	middle-down	1712:1722	arg1	method					1728:1733	the middle-down NMR method	1708:1733	the middle-down NMR method	1708:1733	Therefore, the middle-down NMR method provided a facile orthogonal measurement for mAb glycosylation characterization with improved chemical information content on glycan structure determination and quantification, compared to standard approaches.					
30102512	5	116	theme	mAb	1047:1049	arg1	drugs					1051:1055	marketed mAb drugs	1038:1055	marketed mAb drugs	1038:1055	Here, a "middle-down" NMR method is developed as a proof-of-concept approach to measure the domain-specific glycosylation of marketed mAb drugs without cleavage of the glycan moieties.					
30102512	3	117	theme	mAb	555:557	arg1	labeling					569:576	chemical labeling	560:576	chemical labeling of the glycans	560:591	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	117	theme	mAb	555:557	arg1	separation					601:610	column separation	594:610	column separation	594:610	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	117	theme	mAb	555:557	arg1	cleavage					516:523	glycan cleavage	509:523	glycan cleavage	509:523	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	3	117	theme	mAb	555:557	arg1	digestion					538:546	extensive digestion	528:546	extensive digestion of the mAb	528:557	The established fluorescence or mass-spectrometry (MS) detection methods for glycosylation evaluation may require multiple steps of glycan cleavage or extensive digestion of the mAb, chemical labeling of the glycans, column separation and report the chemical identity of glycans indirectly through retention time and molecular weight values.					
30102512	1	118	theme	drug	248:251	arg1	efficacy					253:260	mAb drug efficacy	244:260	mAb drug efficacy	244:260	Glycosylation of monoclonal antibodies (mAbs) is a critical quality attribute that can impact mAb drug efficacy and safety.					
29433940	4	0	theme	Sialic	368:373	arg1	acid					375:378	Sialic acid	368:378	Sialic acid on N-glycans	368:391	Sialic acid on N-glycans plays important roles in protein functions.					
29433940	8	1	theme	di-sialylated	945:957	arg1	structures					968:977	additional di-sialylated N-glycan structures	934:977	additional di-sialylated N-glycan structures	934:977	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	8	2	theme	additional	934:943	arg1	structures					968:977	additional di-sialylated N-glycan structures	934:977	additional di-sialylated N-glycan structures	934:977	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	8	3	theme	structures	968:977	arg1	formation					921:929	the formation	917:929	the formation of additional di-sialylated N-glycan structures	917:977	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	1	4	theme	silkworm-baculovirus	68:87	arg1	system					89:94	A silkworm-baculovirus system	66:94	A silkworm-baculovirus system	66:94	A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins.					
29433940	1	4	theme	silkworm-baculovirus	68:87	arg1	effective					112:120	effective	112:120	effective	112:120	A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins.					
29433940	8	5	theme	N-glycan	959:966	arg1	structures					968:977	additional di-sialylated N-glycan structures	934:977	additional di-sialylated N-glycan structures	934:977	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	9	6	theme	new	1000:1002	arg1	information					1004:1014	new information	1000:1014	new information on the control of N-glycosylation in silkworm	1000:1060	Our results provide new information on the control of N-glycosylation in silkworm.					
29433940	5	7	from	pathways	461:468	arg1	silkworm					509:516	silkworm	509:516	silkworm	509:516	Therefore, we developed pathways for galactosylation and sialylation in silkworm.					
29433940	6	8	theme	substrate	710:718	arg1	supply					678:683	an external supply	666:683	an external supply of a sialylation-related substrate	666:718	Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate.					
29433940	7	9	theme	α2,3/α2,6	721:729	arg1	Sialylation					731:741	α2,3/α2,6 Sialylation	721:741	α2,3/α2,6 Sialylation to N-glycans	721:754	α2,3/α2,6 Sialylation to N-glycans was controlled by changing the type of sialyltransferase expressed in silkworm.					
29433940	1	10	gly	glycoproteins	168:180	arg1	glycoproteins					168:180	glycoproteins	168:180	glycoproteins	168:180	A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins.					
29433940	0	11	theme	N-glycan	0:7	arg1	sialylation					9:19	N-glycan sialylation	0:19	N-glycan sialylation	0:19	N-glycan sialylation in a silkworm-baculovirus expression system.					
29433940	4	12	theme	protein	418:424	arg1	functions					426:434	protein functions	418:434	protein functions	418:434	Sialic acid on N-glycans plays important roles in protein functions.					
29433940	0	13	theme	expression	47:56	arg1	system					58:63	a silkworm-baculovirus expression system	24:63	a silkworm-baculovirus expression system	24:63	N-glycan sialylation in a silkworm-baculovirus expression system.					
29433940	9	14	from	information	1004:1014	arg1	control					1023:1029	the control	1019:1029	the control of N-glycosylation in silkworm	1019:1060	Our results provide new information on the control of N-glycosylation in silkworm.					
29433940	3	15	theme	pauci-mannose	296:308	arg1	N-glycans					315:323	pauci-mannose type N-glycans	296:323	pauci-mannose type N-glycans without sialic acid or galactose residues	296:365	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	3	15	theme	pauci-mannose	296:308	arg1	Glycoproteins					254:266	Glycoproteins	254:266	Glycoproteins in silkworm	254:278	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	0	16	theme	silkworm-baculovirus	26:45	arg1	system					58:63	a silkworm-baculovirus expression system	24:63	a silkworm-baculovirus expression system	24:63	N-glycan sialylation in a silkworm-baculovirus expression system.					
29433940	3	17	theme	type	310:313	arg1	N-glycans					315:323	pauci-mannose type N-glycans	296:323	pauci-mannose type N-glycans without sialic acid or galactose residues	296:365	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	3	17	theme	type	310:313	arg1	Glycoproteins					254:266	Glycoproteins	254:266	Glycoproteins in silkworm	254:278	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	7	18	theme	sialyltransferase	795:811	arg1	type					787:790	the type	783:790	the type of sialyltransferase expressed in silkworm	783:833	α2,3/α2,6 Sialylation to N-glycans was controlled by changing the type of sialyltransferase expressed in silkworm.					
29433940	8	19	theme	N-acetylglucosaminyltransferase	870:900	arg1	co-expression					853:865	the co-expression	849:865	the co-expression of N-acetylglucosaminyltransferase II	849:903	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	2	20	theme	N-glycan	192:199	arg1	structures					201:210	N-glycan structures	192:210	N-glycan structures in silkworm	192:222	However, N-glycan structures in silkworm differ from those in mammals.					
29433940	6	21	theme	Sialylated	519:528	arg1	N-glycans					530:538	Sialylated N-glycans	519:538	Sialylated N-glycans on proteins	519:550	Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate.					
29433940	1	22	theme	recombinant	136:146	arg1	proteins					148:155	recombinant proteins	136:155	recombinant proteins	136:155	A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins.					
29433940	1	22	theme	recombinant	136:146	arg1	glycoproteins					168:180	glycoproteins	168:180	glycoproteins	168:180	A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins.					
29433940	3	23	from	Glycoproteins	254:266	arg1	silkworm					271:278	silkworm	271:278	silkworm	271:278	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	3	24	theme	galactose	348:356	arg1	residues					358:365	galactose residues	348:365	galactose residues	348:365	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	6	25	theme	external	669:676	arg1	supply					678:683	an external supply	666:683	an external supply of a sialylation-related substrate	666:718	Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate.					
29433940	4	26	theme	important	399:407	arg1	roles					409:413	important roles	399:413	important roles	399:413	Sialic acid on N-glycans plays important roles in protein functions.					
29433940	6	27	from	N-glycans	530:538	arg1	proteins					543:550	proteins	543:550	proteins	543:550	Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate.					
29433940	3	28	theme	sialic	333:338	arg1	acid					340:343	sialic acid	333:343	sialic acid	333:343	Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues.					
29433940	4	29	from	acid	375:378	arg1	N-glycans					383:391	N-glycans	383:391	N-glycans	383:391	Sialic acid on N-glycans plays important roles in protein functions.					
29433940	9	30	theme	N-glycosylation	1034:1048	arg1	control					1023:1029	the control	1019:1029	the control of N-glycosylation in silkworm	1019:1060	Our results provide new information on the control of N-glycosylation in silkworm.					
29433940	8	31	gly	di-sialylated	945:957	arg1	structures					968:977	additional di-sialylated N-glycan structures	934:977	additional di-sialylated N-glycan structures	934:977	Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures.					
29433940	2	32	from	structures	201:210	arg1	silkworm					215:222	silkworm	215:222	silkworm	215:222	However, N-glycan structures in silkworm differ from those in mammals.					
29433940	0	33	dep	sialylation	9:19	arg1	system					58:63	a silkworm-baculovirus expression system	24:63	a silkworm-baculovirus expression system	24:63	N-glycan sialylation in a silkworm-baculovirus expression system.					
29433940	6	34	theme	sialylation-related	690:708	arg1	substrate					710:718	a sialylation-related substrate	688:718	a sialylation-related substrate	688:718	Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate.					
29433940	9	35	from	control	1023:1029	arg1	silkworm					1053:1060	silkworm	1053:1060	silkworm	1053:1060	Our results provide new information on the control of N-glycosylation in silkworm.					
31332757	4	0	theme	payload	775:781	arg1	drug					783:786	an alkyne-tagged payload drug	758:786	an alkyne-tagged payload drug to give a well-defined gsADC	758:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	2	1	theme	structural	386:395	arg1	optimization					397:408	structural optimization	386:408	structural optimization	386:408	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	6	2	dep	enable	1108:1113	arg1	both					1103:1106	both	1103:1106	both	1103:1106	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	5	3	theme	aldehyde	999:1006	arg1	handle					1008:1013	an aldehyde handle	996:1013	an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload	996:1079	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	1	4	theme	tumor-targeting	173:187	arg1	antibody					189:196	a tumor-targeting antibody	171:196	a tumor-targeting antibody	171:196	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	2	5	with	N-glycans	337:345	arg1	homogeneity					357:367	high homogeneity	352:367	high homogeneity that facilitates structural optimization and quality control for ADC drug development	352:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	6	6	theme	single	1234:1239	arg1	mass					1241:1244	a single mass	1232:1244	a single mass under mass-spectral detection	1232:1274	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	6	7	with	gsADCs	1115:1120	arg1	homogeneity					1132:1142	high homogeneity	1127:1142	high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection	1127:1274	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	2	8	theme	payload	311:317	arg1	drugs					319:323	payload drugs	311:323	payload drugs	311:323	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	4	9	theme	Fc	644:645	arg1	glycosites					647:656	Fc glycosites	644:656	Fc glycosites of a therapeutic antibody	644:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	9	theme	Fc	644:645	arg1	antibody					675:682	a therapeutic antibody	661:682	a therapeutic antibody	661:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	10	theme	alkyne-tagged	761:773	arg1	drug					783:786	an alkyne-tagged payload drug	758:786	an alkyne-tagged payload drug to give a well-defined gsADC	758:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	6	11	theme	payload	1172:1178	arg1	numbers					1180:1186	payload numbers	1172:1186	payload numbers	1172:1186	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	5	12	theme	aminooxy-assembled	1054:1071	arg1	payload					1073:1079	an aminooxy-assembled payload	1051:1079	an aminooxy-assembled payload	1051:1079	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	1	13	theme	antibody	189:196	arg1	antibody					189:196	a tumor-targeting antibody	171:196	a tumor-targeting antibody	171:196	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	13	theme	antibody	189:196	arg1	residues					159:166	Lys or Cys residues	148:166	residues	159:166	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	5	14	from	handle	1008:1013	arg1	glycans					1022:1028	the glycans	1018:1028	the glycans for conjugation with an aminooxy-assembled payload	1018:1079	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	15	theme	natural	877:883	arg1	N-glycan					896:903	a natural sialylated N-glycan	875:903	a natural sialylated N-glycan	875:903	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	16	theme	sialic	943:948	arg1	moieties					955:962	sialic acid moieties	943:962	sialic acid moieties using sodium periodate	943:985	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	17	theme	acid	950:953	arg1	moieties					955:962	sialic acid moieties	943:962	sialic acid moieties using sodium periodate	943:985	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	0	18	theme	Homogeneous	0:10	arg1	Antibody-Drug					12:24	Homogeneous Antibody-Drug	0:24	Homogeneous Antibody-Drug	0:24	Homogeneous Antibody-Drug Conjugates via Glycoengineering.					
31332757	4	19	theme	N-glycan	605:612	arg1	substrate					614:622	an azido-tagged unnatural N-glycan substrate	579:622	an azido-tagged unnatural N-glycan substrate	579:622	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	2	20	theme	Fc	334:335	arg1	N-glycans					337:345	IgG Fc N-glycans	330:345	IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development	330:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	2	21	theme	quality	414:420	arg1	control					422:428	quality control	414:428	quality control	414:428	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	5	22	theme	sialylated	885:894	arg1	N-glycan					896:903	a natural sialylated N-glycan	875:903	a natural sialylated N-glycan	875:903	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	4	23	theme	unnatural	595:603	arg1	substrate					614:622	an azido-tagged unnatural N-glycan substrate	579:622	an azido-tagged unnatural N-glycan substrate	579:622	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	24	gly	glycosites	647:656	arg2	antibody					675:682	a therapeutic antibody	661:682	a therapeutic antibody	661:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	24	gly	glycosites	647:656	arg2	glycosites					647:656	Fc glycosites	644:656	Fc glycosites of a therapeutic antibody	644:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	24	gly	glycosites	647:656	arg1	antibody					675:682	a therapeutic antibody	661:682	a therapeutic antibody	661:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	2	25	theme	IgG	330:332	arg1	N-glycans					337:345	IgG Fc N-glycans	330:345	IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development	330:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	4	26	theme	Endo-S-catalyzed	692:707	arg1	glycoremodeling					709:723	Endo-S-catalyzed glycoremodeling	692:723	Endo-S-catalyzed glycoremodeling	692:723	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	27	theme	well-defined	798:809	arg1	gsADC					811:815	a well-defined gsADC	796:815	a well-defined gsADC	796:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	5	28	with	glycoengineering	841:856	arg1	oxidation					930:938	successive selective oxidation	909:938	successive selective oxidation	909:938	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	28	with	glycoengineering	841:856	arg1	N-glycan					896:903	a natural sialylated N-glycan	875:903	a natural sialylated N-glycan	875:903	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	4	29	with	reaction	744:751	arg1	drug					783:786	an alkyne-tagged payload drug	758:786	an alkyne-tagged payload drug to give a well-defined gsADC	758:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	3	30	theme	chemoenzymatic	539:552	arg1	glycoengineering					554:569	chemoenzymatic glycoengineering	539:569	chemoenzymatic glycoengineering	539:569	In this protocol, we report two strategies for preparation of homogeneous ADCs via chemoenzymatic glycoengineering.					
31332757	5	31	theme	successive	909:918	arg1	oxidation					930:938	successive selective oxidation	909:938	successive selective oxidation	909:938	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	3	32	theme	ADCs	530:533	arg1	preparation					503:513	preparation	503:513	preparation of homogeneous ADCs via chemoenzymatic glycoengineering	503:569	In this protocol, we report two strategies for preparation of homogeneous ADCs via chemoenzymatic glycoengineering.					
31332757	5	33	theme	selective	920:928	arg1	oxidation					930:938	successive selective oxidation	909:938	successive selective oxidation	909:938	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	34	with	conjugation	1034:1044	arg1	payload					1073:1079	an aminooxy-assembled payload	1051:1079	an aminooxy-assembled payload	1051:1079	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	4	35	theme	click	738:742	arg1	reaction					744:751	click reaction	738:751	click reaction with an alkyne-tagged payload drug to give a well-defined gsADC	738:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	1	36	theme	payload	230:236	arg1	numbers					238:244	payload numbers	230:244	payload numbers	230:244	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	2	37	theme	ADC	434:436	arg1	development					443:453	ADC drug development	434:453	ADC drug development	434:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	2	38	theme	drug	438:441	arg1	development					443:453	ADC drug development	434:453	ADC drug development	434:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	5	39	theme	antibody	861:868	arg1	glycoengineering					841:856	glycoengineering	841:856	glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate	841:985	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	4	40	dep	transferred	627:637	arg1	followed					726:733	followed	726:733	followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC	726:815	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	3	41	theme	homogeneous	518:528	arg1	ADCs					530:533	homogeneous ADCs	518:533	homogeneous ADCs	518:533	In this protocol, we report two strategies for preparation of homogeneous ADCs via chemoenzymatic glycoengineering.					
31332757	4	42	theme	azido-tagged	582:593	arg1	substrate					614:622	an azido-tagged unnatural N-glycan substrate	579:622	an azido-tagged unnatural N-glycan substrate	579:622	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	5	43	gly	sialylated	885:894	arg1	N-glycan					896:903	a natural sialylated N-glycan	875:903	a natural sialylated N-glycan	875:903	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	1	44	from	heterogeneity	213:225	arg1	positions					262:270	conjugation positions	250:270	conjugation positions	250:270	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	44	from	heterogeneity	213:225	arg1	numbers					238:244	payload numbers	230:244	payload numbers	230:244	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	45	theme	Lys	148:150	arg1	antibody					189:196	a tumor-targeting antibody	171:196	a tumor-targeting antibody	171:196	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	45	theme	Lys	148:150	arg1	residues					159:166	Lys or Cys residues	148:166	residues	159:166	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	46	theme	antibody-drug	72:84	arg1	ADCs					98:101	ADCs	98:101	ADCs	98:101	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	46	theme	antibody-drug	72:84	arg1	conjugates					86:95	Conventional antibody-drug conjugates	59:95	Conventional antibody-drug conjugates (ADCs)	59:102	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	5	47	theme	moieties	955:962	arg1	oxidation					930:938	successive selective oxidation	909:938	successive selective oxidation	909:938	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	5	47	theme	moieties	955:962	arg1	N-glycan					896:903	a natural sialylated N-glycan	875:903	a natural sialylated N-glycan	875:903	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	2	48	theme	high	352:355	arg1	homogeneity					357:367	high homogeneity	352:367	high homogeneity that facilitates structural optimization and quality control for ADC drug development	352:453	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	1	49	theme	small-molecule	122:135	arg1	drugs					137:141	small-molecule drugs	122:141	small-molecule drugs	122:141	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	6	50	theme	conjugation	1153:1163	arg1	sites					1165:1169	their conjugation sites	1147:1169	their conjugation sites	1147:1169	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	1	51	theme	Cys	155:157	arg1	antibody					189:196	a tumor-targeting antibody	171:196	a tumor-targeting antibody	171:196	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	51	theme	Cys	155:157	arg1	residues					159:166	Lys or Cys residues	148:166	residues	159:166	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	6	52	from	homogeneity	1132:1142	arg1	sites					1165:1169	their conjugation sites	1147:1169	their conjugation sites	1147:1169	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	6	52	from	homogeneity	1132:1142	arg1	numbers					1180:1186	payload numbers	1172:1186	payload numbers	1172:1186	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	6	52	from	homogeneity	1132:1142	arg1	glycoforms					1193:1202	glycoforms	1193:1202	glycoforms	1193:1202	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	1	53	theme	conjugation	250:260	arg1	positions					262:270	conjugation positions	250:270	conjugation positions	250:270	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	6	54	theme	high	1127:1130	arg1	homogeneity					1132:1142	high homogeneity	1127:1142	high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection	1127:1274	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	1	55	theme	Conventional	59:70	arg1	ADCs					98:101	ADCs	98:101	ADCs	98:101	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	1	55	theme	Conventional	59:70	arg1	conjugates					86:95	Conventional antibody-drug conjugates	59:95	Conventional antibody-drug conjugates (ADCs)	59:102	Conventional antibody-drug conjugates (ADCs) randomly assemble small-molecule drugs onto Lys or Cys residues of a tumor-targeting antibody, featured with heterogeneity in payload numbers and conjugation positions.					
31332757	6	56	theme	mass-spectral	1252:1264	arg1	detection					1266:1274	mass-spectral detection	1252:1274	mass-spectral detection	1252:1274	These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-spectral detection.					
31332757	4	57	theme	antibody	675:682	arg1	glycosites					647:656	Fc glycosites	644:656	Fc glycosites of a therapeutic antibody	644:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	4	57	theme	antibody	675:682	arg1	antibody					675:682	a therapeutic antibody	661:682	a therapeutic antibody	661:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
31332757	5	58	theme	alternative	824:834	arg1	way					836:838	an alternative way	821:838	an alternative way	821:838	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	2	59	theme	Glycosite-specific	273:290	arg1	ADCs					292:295	Glycosite-specific ADCs	273:295	Glycosite-specific ADCs (gsADCs)	273:304	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	2	59	theme	Glycosite-specific	273:290	arg1	gsADCs					298:303	gsADCs	298:303	gsADCs	298:303	Glycosite-specific ADCs (gsADCs) link payload drugs onto IgG Fc N-glycans with high homogeneity that facilitates structural optimization and quality control for ADC drug development.					
31332757	5	60	theme	sodium	970:975	arg1	periodate					977:985	sodium periodate	970:985	sodium periodate	970:985	In an alternative way, glycoengineering of antibody with a natural sialylated N-glycan and successive selective oxidation of sialic acid moieties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload.					
31332757	4	61	theme	therapeutic	663:673	arg1	antibody					675:682	a therapeutic antibody	661:682	a therapeutic antibody	661:682	First, an azido-tagged unnatural N-glycan substrate is transferred onto Fc glycosites of a therapeutic antibody through Endo-S-catalyzed glycoremodeling, followed by click reaction with an alkyne-tagged payload drug to give a well-defined gsADC.					
30899004	5	0	theme	dramatic	1041:1048	arg1	differences					1050:1060	dramatic differences	1041:1060	dramatic differences in glycosites on the same protein	1041:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	6	1	gly	glycopeptides	1232:1244	arg2	glycopeptides					1232:1244	intact glycopeptides	1225:1244	intact glycopeptides	1225:1244	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	6	2	theme	glycoproteomic	1131:1144	arg1	dataset					1146:1152	this large-scale glycoproteomic dataset	1114:1152	this large-scale glycoproteomic dataset	1114:1152	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	6	3	theme	future	1249:1254	arg1	studies					1256:1262	future studies	1249:1262	future studies	1249:1262	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	4	4	theme	site-specific	586:598	arg1	microheterogeneity					600:617	N-glycoproteome site-specific microheterogeneity	570:617	N-glycoproteome site-specific microheterogeneity	570:617	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	5	gly	N-glycosites	780:791	arg2	N-glycosites					780:791	1,545 N-glycosites	774:791	1,545 N-glycosites (>5,600 unique N-glycopeptides)	774:823	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	5	gly	N-glycosites	780:791	arg2	N-glycopeptides					808:822	>5,600 unique N-glycopeptides	794:822	>5,600 unique N-glycopeptides	794:822	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	5	6	from	differences	1050:1060	arg1	glycosites					1065:1074	glycosites	1065:1074	glycosites on the same protein	1065:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	6	from	differences	1050:1060	arg1	protein					1088:1094	the same protein	1079:1094	the same protein	1079:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	1	7	theme	Protein	81:87	arg1	glycosylation					89:101	Protein glycosylation	81:101	Protein glycosylation	81:101	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	1	7	theme	Protein	81:87	arg1	modification					175:186	a highly important, yet poorly understood protein post-translational modification	106:186	a highly important, yet poorly understood protein post-translational modification	106:186	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	4	8	theme	ion	703:705	arg1	AI-ETD					739:744	AI-ETD	739:744	AI-ETD	739:744	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	8	theme	ion	703:705	arg1	dissociation					725:736	activated ion electron transfer dissociation	693:736	activated ion electron transfer dissociation (AI-ETD)	693:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	6	9	theme	large-scale	1119:1129	arg1	dataset					1146:1152	this large-scale glycoproteomic dataset	1114:1152	this large-scale glycoproteomic dataset	1114:1152	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	4	10	theme	brain	836:840	arg1	tissue					842:847	mouse brain tissue	830:847	mouse brain tissue	830:847	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	5	11	gly	N-glycosite	967:977	arg2	N-glycosite					967:977	N-glycosite heterogeneity	967:991	N-glycosite heterogeneity	967:991	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	6	12	theme	intact	1225:1230	arg1	glycopeptides					1232:1244	intact glycopeptides	1225:1244	intact glycopeptides	1225:1244	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	4	13	theme	1,545	774:778	arg1	N-glycopeptides					808:822	>5,600 unique N-glycopeptides	794:822	>5,600 unique N-glycopeptides	794:822	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	13	theme	1,545	774:778	arg1	N-glycosites					780:791	1,545 N-glycosites	774:791	1,545 N-glycosites (>5,600 unique N-glycopeptides)	774:823	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	14	theme	activated	693:701	arg1	AI-ETD					739:744	AI-ETD	739:744	AI-ETD	739:744	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	14	theme	activated	693:701	arg1	dissociation					725:736	activated ion electron transfer dissociation	693:736	activated ion electron transfer dissociation (AI-ETD)	693:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	3	15	theme	suitable	308:315	arg1	methods					328:334	suitable analytical methods	308:334	suitable analytical methods for large-scale analyses of intact glycopeptides	308:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	5	16	theme	several	1006:1012	arg1	differences					1050:1060	dramatic differences	1041:1060	dramatic differences in glycosites on the same protein	1041:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	16	theme	several	1006:1012	arg1	forms					1024:1028	several different forms	1006:1028	several different forms	1006:1028	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	0	17	theme	site-specific	10:22	arg1	heterogeneity					24:36	site-specific heterogeneity	10:36	site-specific heterogeneity	10:36	Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis.					
30899004	5	18	theme	same	1083:1086	arg1	protein					1088:1094	the same protein	1079:1094	the same protein	1079:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	19	theme	different	1014:1022	arg1	differences					1050:1060	dramatic differences	1041:1060	dramatic differences in glycosites on the same protein	1041:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	19	theme	different	1014:1022	arg1	forms					1024:1028	several different forms	1006:1028	several different forms	1006:1028	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	20	theme	structural	939:948	arg1	domains					950:956	structural domains	939:956	structural domains	939:956	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	4	21	theme	glycopeptide	651:662	arg1	methods					674:680	large-scale glycopeptide profiling methods	639:680	large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD)	639:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	1	22	theme	important	115:123	arg1	glycosylation					89:101	Protein glycosylation	81:101	Protein glycosylation	81:101	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	1	22	theme	important	115:123	arg1	modification					175:186	a highly important, yet poorly understood protein post-translational modification	106:186	a highly important, yet poorly understood protein post-translational modification	106:186	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	4	23	theme	large-scale	639:649	arg1	methods					674:680	large-scale glycopeptide profiling methods	639:680	large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD)	639:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	5	24	gly	glycosites	1065:1074	arg2	glycosites					1065:1074	glycosites	1065:1074	glycosites on the same protein	1065:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	5	25	from	glycosites	1065:1074	arg1	protein					1088:1094	the same protein	1079:1094	the same protein	1079:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	3	26	theme	complex	535:541	arg1	systems					543:549	complex systems	535:549	complex systems	535:549	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	3	27	theme	large-scale	340:350	arg1	analyses					352:359	large-scale analyses	340:359	large-scale analyses of intact glycopeptides	340:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	5	28	theme	N-glycosite	967:977	arg1	heterogeneity					979:991	N-glycosite heterogeneity	967:991	N-glycosite heterogeneity	967:991	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	0	29	theme	N-glycoproteome	55:69	arg1	analysis					71:78	large-scale N-glycoproteome analysis	43:78	large-scale N-glycoproteome analysis	43:78	Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis.					
30899004	4	30	theme	profiling	664:672	arg1	methods					674:680	large-scale glycopeptide profiling methods	639:680	large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD)	639:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	3	31	theme	analytical	317:326	arg1	methods					328:334	suitable analytical methods	308:334	suitable analytical methods for large-scale analyses of intact glycopeptides	308:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	2	32	theme	structures	218:227	arg1	Thousands					189:197	Thousands	189:197	Thousands of possible glycan structures and compositions	189:244	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	0	33	theme	large-scale	43:53	arg1	analysis					71:78	large-scale N-glycoproteome analysis	43:78	large-scale N-glycoproteome analysis	43:78	Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis.					
30899004	3	34	theme	methods	328:334	arg1	lack					300:303	A lack	298:303	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides	298:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	2	35	theme	glycan	211:216	arg1	structures					218:227	possible glycan structures	202:227	possible glycan structures	202:227	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	5	36	theme	subcellular	915:925	arg1	regions					927:933	subcellular regions	915:933	subcellular regions	915:933	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	3	37	theme	intact	364:369	arg1	glycopeptides					371:383	intact glycopeptides	364:383	intact glycopeptides	364:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	2	38	theme	possible	202:209	arg1	structures					218:227	possible glycan structures	202:227	possible glycan structures	202:227	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	2	39	theme	site	278:281	arg1	heterogeneity					283:295	tremendous site heterogeneity	267:295	tremendous site heterogeneity	267:295	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	4	40	gly	glycopeptide	651:662	arg2	glycopeptide					651:662	large-scale glycopeptide profiling methods	639:680	large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD)	639:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	41	theme	mouse	830:834	arg1	tissue					842:847	mouse brain tissue	830:847	mouse brain tissue	830:847	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	3	42	theme	glycopeptides	371:383	arg1	analyses					352:359	large-scale analyses	340:359	large-scale analyses of intact glycopeptides	340:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	2	43	theme	tremendous	267:276	arg1	heterogeneity					283:295	tremendous site heterogeneity	267:295	tremendous site heterogeneity	267:295	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	1	44	theme	understood	137:146	arg1	glycosylation					89:101	Protein glycosylation	81:101	Protein glycosylation	81:101	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	1	44	theme	understood	137:146	arg1	modification					175:186	a highly important, yet poorly understood protein post-translational modification	106:186	a highly important, yet poorly understood protein post-translational modification	106:186	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	4	45	gly	N-glycopeptides	808:822	arg2	N-glycosites					780:791	1,545 N-glycosites	774:791	1,545 N-glycosites (>5,600 unique N-glycopeptides)	774:823	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	45	gly	N-glycopeptides	808:822	arg2	N-glycopeptides					808:822	>5,600 unique N-glycopeptides	794:822	>5,600 unique N-glycopeptides	794:822	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	46	theme	>5,600	794:799	arg1	N-glycopeptides					808:822	>5,600 unique N-glycopeptides	794:822	>5,600 unique N-glycopeptides	794:822	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	46	theme	>5,600	794:799	arg1	N-glycosites					780:791	1,545 N-glycosites	774:791	1,545 N-glycosites (>5,600 unique N-glycopeptides)	774:823	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	1	47	theme	protein	148:154	arg1	glycosylation					89:101	Protein glycosylation	81:101	Protein glycosylation	81:101	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	1	47	theme	protein	148:154	arg1	modification					175:186	a highly important, yet poorly understood protein post-translational modification	106:186	a highly important, yet poorly understood protein post-translational modification	106:186	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	4	48	theme	transfer	716:723	arg1	AI-ETD					739:744	AI-ETD	739:744	AI-ETD	739:744	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	48	theme	transfer	716:723	arg1	dissociation					725:736	activated ion electron transfer dissociation	693:736	activated ion electron transfer dissociation (AI-ETD)	693:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	1	49	theme	post-translational	156:173	arg1	glycosylation					89:101	Protein glycosylation	81:101	Protein glycosylation	81:101	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	1	49	theme	post-translational	156:173	arg1	modification					175:186	a highly important, yet poorly understood protein post-translational modification	106:186	a highly important, yet poorly understood protein post-translational modification	106:186	Protein glycosylation is a highly important, yet poorly understood protein post-translational modification.					
30899004	4	50	theme	electron	707:714	arg1	AI-ETD					739:744	AI-ETD	739:744	AI-ETD	739:744	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	50	theme	electron	707:714	arg1	dissociation					725:736	activated ion electron transfer dissociation	693:736	activated ion electron transfer dissociation (AI-ETD)	693:745	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	3	51	gly	glycopeptides	371:383	arg2	glycopeptides					371:383	intact glycopeptides	364:383	intact glycopeptides	364:383	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30899004	2	52	theme	compositions	233:244	arg1	Thousands					189:197	Thousands	189:197	Thousands of possible glycan structures and compositions	189:244	Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity.					
30899004	4	53	theme	unique	801:806	arg1	N-glycopeptides					808:822	>5,600 unique N-glycopeptides	794:822	>5,600 unique N-glycopeptides	794:822	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	4	53	theme	unique	801:806	arg1	N-glycosites					780:791	1,545 N-glycosites	774:791	1,545 N-glycosites (>5,600 unique N-glycopeptides)	774:823	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	5	54	from	protein	1088:1094	arg1	differences					1050:1060	dramatic differences	1041:1060	dramatic differences in glycosites on the same protein	1041:1094	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	6	55	theme	several	1165:1171	arg1	visualizations					1173:1186	several visualizations	1165:1186	several visualizations that will prove useful for analyzing intact glycopeptides in future studies	1165:1262	Moreover, we use this large-scale glycoproteomic dataset to develop several visualizations that will prove useful for analyzing intact glycopeptides in future studies.					
30899004	5	56	theme	N-glycosylation	871:885	arg1	profiles					887:894	N-glycosylation profiles	871:894	N-glycosylation profiles	871:894	Our data reveal that N-glycosylation profiles can differ between subcellular regions and structural domains and that N-glycosite heterogeneity manifests in several different forms, including dramatic differences in glycosites on the same protein.					
30899004	4	57	theme	N-glycoproteome	570:584	arg1	microheterogeneity					600:617	N-glycoproteome site-specific microheterogeneity	570:617	N-glycoproteome site-specific microheterogeneity	570:617	Here we show that N-glycoproteome site-specific microheterogeneity can be captured via large-scale glycopeptide profiling methods enabled by activated ion electron transfer dissociation (AI-ETD), ultimately characterizing 1,545 N-glycosites (>5,600 unique N-glycopeptides) from mouse brain tissue.					
30899004	3	58	theme	heterogeneity	441:453	arg1	degree					431:436	the degree	427:436	the degree of heterogeneity across the glycoproteome	427:478	A lack of suitable analytical methods for large-scale analyses of intact glycopeptides has limited our abilities both to address the degree of heterogeneity across the glycoproteome and to understand how this contributes biologically to complex systems.					
30643095	6	0	theme	glycan	748:753	arg1	processing					755:764	glycan processing	748:764	glycan processing	748:764	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	4	1	theme	clear	389:393	arg1	relationship					395:406	A clear relationship	387:406	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies	387:497	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	4	2	theme	dystrophies	487:497	arg1	glycans					427:433	O-mannosyl glycans	416:433	O-mannosyl glycans	416:433	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	4	2	theme	dystrophies	487:497	arg1	pathomechanisms					443:457	the pathomechanisms	439:457	the pathomechanisms of some congenital muscular dystrophies	439:497	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	4	3	theme	muscular	478:485	arg1	dystrophies					487:497	some congenital muscular dystrophies	462:497	some congenital muscular dystrophies	462:497	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	6	4	theme	mechanisms	734:743	arg1	identification					699:712	the identification	695:712	the identification of highly regulated mechanisms of glycan processing	695:764	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	6	4	theme	mechanisms	734:743	arg1	discovery					656:664	The discovery	652:664	The discovery of new glycan structures	652:689	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	6	5	theme	therapeutic	831:841	arg1	strategies					843:852	therapeutic strategies	831:852	therapeutic strategies	831:852	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	1	6	theme	important	83:91	arg1	modification					111:122	an important posttranslational modification	80:122	an important posttranslational modification in mammals	80:133	Glycosylation is an important posttranslational modification in mammals.					
30643095	1	6	theme	important	83:91	arg1	Glycosylation					63:75	Glycosylation	63:75	Glycosylation	63:75	Glycosylation is an important posttranslational modification in mammals.					
30643095	2	7	gly	glycoproteins	151:163	arg1	glycoproteins					151:163	glycoproteins	151:163	glycoproteins	151:163	The glycans of glycoproteins are classified into two groups, namely, N-glycans and O-glycans, according to their glycan-peptide linkage regions.					
30643095	0	8	theme	O-mannosyl	10:19	arg1	glycans					21:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans: Biochemistry and glycopathology.					
30643095	2	9	theme	glycoproteins	151:163	arg1	glycans					140:146	The glycans	136:146	The glycans of glycoproteins	136:163	The glycans of glycoproteins are classified into two groups, namely, N-glycans and O-glycans, according to their glycan-peptide linkage regions.					
30643095	1	10	theme	posttranslational	93:109	arg1	modification					111:122	an important posttranslational modification	80:122	an important posttranslational modification in mammals	80:133	Glycosylation is an important posttranslational modification in mammals.					
30643095	1	10	theme	posttranslational	93:109	arg1	Glycosylation					63:75	Glycosylation	63:75	Glycosylation	63:75	Glycosylation is an important posttranslational modification in mammals.					
30643095	3	11	from	brain	368:372	arg1	important					344:352	important	344:352	important	344:352	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	0	12	theme	Mammalian	0:8	arg1	glycans					21:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans: Biochemistry and glycopathology.					
30643095	3	13	theme	O-mannosyl	291:300	arg1	glycan					302:307	O-mannosyl glycan	291:307	O-mannosyl glycan	291:307	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	3	13	theme	O-mannosyl	291:300	arg1	O-glycan					313:320	an O-glycan	310:320	an O-glycan	310:320	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	5	14	theme	biosynthetic	610:621	arg1	pathway					623:629	its biosynthetic pathway	606:629	its biosynthetic pathway	606:629	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	5	15	from	component	580:588	arg1	mammals					593:599	mammals	593:599	mammals	593:599	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	3	16	from	important	344:352	arg1	brain					368:372	brain	368:372	brain	368:372	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	3	16	from	important	344:352	arg1	muscle					357:362	muscle	357:362	muscle	357:362	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	4	17	theme	congenital	467:476	arg1	dystrophies					487:497	some congenital muscular dystrophies	462:497	some congenital muscular dystrophies	462:497	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	5	18	theme	identified	562:571	arg1	Ribitol-5-phosphate					531:549	Ribitol-5-phosphate	531:549	Ribitol-5-phosphate	531:549	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	5	18	theme	identified	562:571	arg1	component					580:588	a newly identified glycan component	554:588	a newly identified glycan component in mammals	554:599	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	3	19	from	muscle	357:362	arg1	important					344:352	important	344:352	important	344:352	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	5	20	theme	glycan	573:578	arg1	Ribitol-5-phosphate					531:549	Ribitol-5-phosphate	531:549	Ribitol-5-phosphate	531:549	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	5	20	theme	glycan	573:578	arg1	component					580:588	a newly identified glycan component	554:588	a newly identified glycan component in mammals	554:599	Ribitol-5-phosphate is a newly identified glycan component in mammals, and its biosynthetic pathway has been elucidated.					
30643095	0	21	dep	Biochemistry	30:41	arg1	glycans					21:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans	0:27	Mammalian O-mannosyl glycans: Biochemistry and glycopathology.					
30643095	6	22	theme	structures	680:689	arg1	identification					699:712	the identification	695:712	the identification of highly regulated mechanisms of glycan processing	695:764	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	6	22	theme	structures	680:689	arg1	discovery					656:664	The discovery	652:664	The discovery of new glycan structures	652:689	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	4	23	theme	O-mannosyl	416:425	arg1	glycans					427:433	O-mannosyl glycans	416:433	O-mannosyl glycans	416:433	A clear relationship between O-mannosyl glycans and the pathomechanisms of some congenital muscular dystrophies has been established in humans.					
30643095	6	24	theme	glycan	673:678	arg1	structures					680:689	new glycan structures	669:689	new glycan structures	669:689	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	3	25	dep	muscle	357:362	arg1	development					374:384	development	374:384	development	374:384	Recently, O-mannosyl glycan, an O-glycan, has been shown to be important in muscle and brain development.					
30643095	6	26	theme	glycan	802:807	arg1	functions					809:817	glycan functions	802:817	glycan functions	802:817	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	6	27	theme	new	669:671	arg1	structures					680:689	new glycan structures	669:689	new glycan structures	669:689	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	6	28	theme	regulated	724:732	arg1	mechanisms					734:743	highly regulated mechanisms	717:743	highly regulated mechanisms of glycan processing	717:764	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
30643095	2	29	theme	linkage	264:270	arg1	regions					272:278	their glycan-peptide linkage regions	243:278	their glycan-peptide linkage regions	243:278	The glycans of glycoproteins are classified into two groups, namely, N-glycans and O-glycans, according to their glycan-peptide linkage regions.					
30643095	1	30	from	modification	111:122	arg1	mammals					127:133	mammals	127:133	mammals	127:133	Glycosylation is an important posttranslational modification in mammals.					
30643095	2	31	theme	glycan-peptide	249:262	arg1	regions					272:278	their glycan-peptide linkage regions	243:278	their glycan-peptide linkage regions	243:278	The glycans of glycoproteins are classified into two groups, namely, N-glycans and O-glycans, according to their glycan-peptide linkage regions.					
30643095	6	32	theme	processing	755:764	arg1	mechanisms					734:743	highly regulated mechanisms	717:743	highly regulated mechanisms of glycan processing	717:764	The discovery of new glycan structures and the identification of highly regulated mechanisms of glycan processing will help researchers to understand glycan functions and develop therapeutic strategies.					
31541622	7	0	theme	high	1160:1163	arg1	confidence					1165:1174	high confidence	1160:1174	high confidence	1160:1174	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	1	1	theme	protein	150:156	arg1	structure					158:166	protein structure	150:166	protein structure	150:166	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	10	2	theme	glycan	1521:1526	arg1	occupancy					1533:1541	glycan site occupancy	1521:1541	glycan site occupancy	1521:1541	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	10	3	from	variations	1507:1516	arg1	occupancy					1533:1541	glycan site occupancy	1521:1541	glycan site occupancy	1521:1541	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	0	4	theme	susceptible	67:77	arg1	strains					79:85	viral resistant and susceptible strains	47:85	viral resistant and susceptible strains of Bombyx mori	47:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	0	5	from	mapping	10:16	arg1	strains					79:85	viral resistant and susceptible strains	47:85	viral resistant and susceptible strains of Bombyx mori	47:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	5	6	theme	importance	856:865	arg1	challenges					823:832	analytical challenges	812:832	analytical challenges	812:832	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	5	6	theme	importance	856:865	arg1	lack					848:851	perceived lack	838:851	perceived lack of importance	838:865	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	6	7	theme	Bombyx	1036:1041	arg1	nucleopolyhedrovirus					1048:1067	the baculovirus Bombyx mori nucleopolyhedrovirus	1020:1067	the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV)	1020:1075	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	7	theme	Bombyx	1036:1041	arg1	BmNPV					1070:1074	BmNPV	1070:1074	BmNPV	1070:1074	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	10	8	theme	infection	1677:1685	arg1	processes					1687:1695	viral recognition and infection processes	1655:1695	viral recognition and infection processes	1655:1695	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	0	9	theme	Bombyx	90:95	arg1	mori					97:100	Bombyx mori	90:100	Bombyx mori	90:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	5	10	from	knowledge	748:756	arg1	glycoproteome					765:777	its glycoproteome	761:777	its glycoproteome	761:777	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	8	11	theme	resistant	1305:1313	arg1	strain					1315:1320	the BmNPV susceptible or resistant strain	1280:1320	the BmNPV susceptible or resistant strain	1280:1320	We mapped some glycoproteins only to the BmNPV susceptible or resistant strain, underlining the potential relationship between glycosylation and viral susceptibility.					
31541622	8	12	theme	potential	1339:1347	arg1	relationship					1349:1360	the potential relationship	1335:1360	the potential relationship between glycosylation and viral susceptibility	1335:1407	We mapped some glycoproteins only to the BmNPV susceptible or resistant strain, underlining the potential relationship between glycosylation and viral susceptibility.					
31541622	6	13	theme	Bombyx	949:954	arg1	strains					961:967	two silkworm Bombyx mori strains	936:967	two silkworm Bombyx mori strains 306 and NB	936:978	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	5	14	theme	model	698:702	arg1	organism					704:711	a model organism	696:711	an important economic insect as well as a model organism for molecular biology	656:733	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	6	15	theme	silkworm	940:947	arg1	strains					961:967	two silkworm Bombyx mori strains	936:967	two silkworm Bombyx mori strains 306 and NB	936:978	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	2	16	theme	glycans	258:264	arg1	roles					249:253	the major roles	239:253	the major roles of glycans and glycoconjugates on the cell surface	239:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	3	17	theme	membrane	445:452	arg1	receptors					454:462	cell membrane receptors	440:462	cell membrane receptors	440:462	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	6	18	theme	mori	1043:1046	arg1	nucleopolyhedrovirus					1048:1067	the baculovirus Bombyx mori nucleopolyhedrovirus	1020:1067	the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV)	1020:1075	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	18	theme	mori	1043:1046	arg1	BmNPV					1070:1074	BmNPV	1070:1074	BmNPV	1070:1074	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	3	19	theme	cases	483:487	arg1	glycoproteins					489:501	many cases glycoproteins	478:501	many cases glycoproteins	478:501	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	3	20	theme	viral	399:403	arg1	replication					405:415	viral replication	399:415	viral replication	399:415	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	0	21	theme	mori	97:100	arg1	strains					79:85	viral resistant and susceptible strains	47:85	viral resistant and susceptible strains of Bombyx mori	47:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	5	22	theme	current	740:746	arg1	knowledge					748:756	current knowledge	740:756	current knowledge on its glycoproteome	740:777	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	2	23	theme	roles	249:253	arg1	receptor					323:330	the receptor	319:330	the receptor for outside pathogens such as viruses	319:368	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	2	23	theme	roles	249:253	arg1	roles					249:253	the major roles	239:253	the major roles of glycans and glycoconjugates on the cell surface	239:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	2	23	theme	roles	249:253	arg1	One					232:234	One	232:234	One	232:234	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	3	24	theme	replication	405:415	arg1	stage					390:394	the initial stage	378:394	the initial stage of viral replication	378:415	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	8	25	gly	glycoproteins	1258:1270	arg1	glycoproteins					1258:1270	some glycoproteins	1253:1270	some glycoproteins	1253:1270	We mapped some glycoproteins only to the BmNPV susceptible or resistant strain, underlining the potential relationship between glycosylation and viral susceptibility.					
31541622	10	26	theme	site	1528:1531	arg1	occupancy					1533:1541	glycan site occupancy	1521:1541	glycan site occupancy	1521:1541	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	6	27	theme	baculovirus	1024:1034	arg1	nucleopolyhedrovirus					1048:1067	the baculovirus Bombyx mori nucleopolyhedrovirus	1020:1067	the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV)	1020:1075	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	27	theme	baculovirus	1024:1034	arg1	BmNPV					1070:1074	BmNPV	1070:1074	BmNPV	1070:1074	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	2	28	theme	cell	293:296	arg1	surface					298:304	the cell surface	289:304	the cell surface	289:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	9	29	theme	O-glycan	1443:1450	arg1	compositions					1452:1463	O-glycan compositions	1443:1463	O-glycan compositions	1443:1463	We predicted O-glycoproteins and O-glycan compositions for the first time for this organism.					
31541622	0	30	theme	Precision	0:8	arg1	mapping					10:16	Precision mapping	0:16	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori	0:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	5	31	theme	analytical	812:821	arg1	challenges					823:832	analytical challenges	812:832	analytical challenges	812:832	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	5	32	theme	perceived	838:846	arg1	lack					848:851	perceived lack	838:851	perceived lack of importance	838:865	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	0	33	theme	N-	21:22	arg1	mapping					10:16	Precision mapping	0:16	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori	0:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	4	34	theme	such	516:519	arg1	glycoproteins					521:533	such glycoproteins	516:533	such glycoproteins	516:533	Identifying such glycoproteins is essential to understanding the mechanisms of viral infection, as well as developing antiviral strategies.					
31541622	1	35	theme	immune	183:188	arg1	recognition					190:200	immune recognition	183:200	immune recognition	183:200	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	7	36	theme	N-	1115:1116	arg1	glycoproteins					1125:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	4	37	theme	viral	583:587	arg1	infection					589:597	viral infection	583:597	viral infection	583:597	Identifying such glycoproteins is essential to understanding the mechanisms of viral infection, as well as developing antiviral strategies.					
31541622	6	38	dep	strains	961:967	arg1	NB					977:978	NB	977:978	NB	977:978	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	38	dep	strains	961:967	arg1	306					969:971	306	969:971	306	969:971	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	2	39	theme	glycoconjugates	270:284	arg1	roles					249:253	the major roles	239:253	the major roles of glycans and glycoconjugates on the cell surface	239:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	10	40	theme	viral	1655:1659	arg1	recognition					1661:1671	viral recognition	1655:1671	viral recognition	1655:1671	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	0	41	theme	O-glycoproteins	28:42	arg1	mapping					10:16	Precision mapping	0:16	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori	0:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	6	42	theme	glycoproteomic	910:923	arg1	susceptible					991:1001	susceptible	991:1001	susceptible	991:1001	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	42	theme	glycoproteomic	910:923	arg1	survey					925:930	a large-scale glycoproteomic survey	896:930	a large-scale glycoproteomic survey	896:930	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	7	43	theme	O-	1122:1123	arg1	glycoproteins					1125:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	9	44	theme	first	1473:1477	arg1	time					1479:1482	the first time	1469:1482	the first time for this organism	1469:1500	We predicted O-glycoproteins and O-glycan compositions for the first time for this organism.					
31541622	3	45	theme	initial	382:388	arg1	stage					390:394	the initial stage	378:394	the initial stage of viral replication	378:415	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	6	46	theme	large-scale	898:908	arg1	susceptible					991:1001	susceptible	991:1001	susceptible	991:1001	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	6	46	theme	large-scale	898:908	arg1	survey					925:930	a large-scale glycoproteomic survey	896:930	a large-scale glycoproteomic survey	896:930	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	10	47	theme	glycan	1555:1560	arg1	diversity					1562:1570	glycan diversity	1555:1570	glycan diversity between the two silkworm strains	1555:1603	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	10	48	gly	glycosylation	1638:1650	arg1	infection					1677:1685	infection	1677:1685	infection	1677:1685	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	10	48	gly	glycosylation	1638:1650	arg1	recognition					1661:1671	viral recognition	1655:1671	viral recognition	1655:1671	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	7	49	theme	silkworm	1106:1113	arg1	glycoproteins					1125:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	3	50	gly	glycoproteins	489:501	arg1	glycoproteins					489:501	many cases glycoproteins	478:501	many cases glycoproteins	478:501	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	10	51	theme	glycosylation	1638:1650	arg1	role					1630:1633	role	1630:1633	role of glycosylation in viral recognition and infection processes	1630:1695	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	0	52	theme	viral	47:51	arg1	strains					79:85	viral resistant and susceptible strains	47:85	viral resistant and susceptible strains of Bombyx mori	47:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	9	53	gly	O-glycoproteins	1423:1437	arg1	O-glycoproteins					1423:1437	O-glycoproteins	1423:1437	O-glycoproteins	1423:1437	We predicted O-glycoproteins and O-glycan compositions for the first time for this organism.					
31541622	8	54	theme	viral	1388:1392	arg1	susceptibility					1394:1407	viral susceptibility	1388:1407	viral susceptibility	1388:1407	We mapped some glycoproteins only to the BmNPV susceptible or resistant strain, underlining the potential relationship between glycosylation and viral susceptibility.					
31541622	4	55	gly	glycoproteins	521:533	arg1	glycoproteins					521:533	such glycoproteins	516:533	such glycoproteins	516:533	Identifying such glycoproteins is essential to understanding the mechanisms of viral infection, as well as developing antiviral strategies.					
31541622	1	56	theme	Protein	103:109	arg1	glycosylation					111:123	Protein glycosylation	103:123	Protein glycosylation	103:123	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	2	57	theme	major	243:247	arg1	roles					249:253	the major roles	239:253	the major roles of glycans and glycoconjugates on the cell surface	239:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	10	58	from	role	1630:1633	arg1	processes					1687:1695	viral recognition and infection processes	1655:1695	viral recognition and infection processes	1655:1695	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	3	59	theme	cell	440:443	arg1	receptors					454:462	cell membrane receptors	440:462	cell membrane receptors	440:462	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	2	60	from	roles	249:253	arg1	surface					298:304	the cell surface	289:304	the cell surface	289:304	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	1	61	theme	many	209:212	arg1	activities					220:229	many other activities	209:229	many other activities	209:229	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	7	62	gly	glycoproteins	1125:1137	arg1	glycoproteins					1125:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins	1092:1137	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	10	63	from	diversity	1562:1570	arg1	occupancy					1533:1541	glycan site occupancy	1521:1541	glycan site occupancy	1521:1541	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	0	64	gly	O-glycoproteins	28:42	arg1	O-glycoproteins					28:42	O-glycoproteins	28:42	O-glycoproteins	28:42	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	1	65	theme	important	131:139	arg1	roles					141:145	important roles	131:145	important roles	131:145	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	8	66	theme	susceptible	1290:1300	arg1	strain					1315:1320	the BmNPV susceptible or resistant strain	1280:1320	the BmNPV susceptible or resistant strain	1280:1320	We mapped some glycoproteins only to the BmNPV susceptible or resistant strain, underlining the potential relationship between glycosylation and viral susceptibility.					
31541622	1	67	theme	other	214:218	arg1	activities					220:229	many other activities	209:229	many other activities	209:229	Protein glycosylation plays important roles in protein structure, function, and immune recognition, among many other activities.					
31541622	5	68	theme	molecular	717:725	arg1	biology					727:733	molecular biology	717:733	molecular biology	717:733	Silkworm is an important economic insect as well as a model organism for molecular biology, yet current knowledge on its glycoproteome is far from complete due to both analytical challenges and perceived lack of importance.					
31541622	10	69	theme	recognition	1661:1671	arg1	processes					1687:1695	viral recognition and infection processes	1655:1695	viral recognition and infection processes	1655:1695	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	2	70	theme	outside	336:342	arg1	pathogens					344:352	outside pathogens	336:352	outside pathogens such as viruses	336:368	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	2	70	theme	outside	336:342	arg1	viruses					362:368	viruses	362:368	viruses	362:368	One of the major roles of glycans and glycoconjugates on the cell surface is acting as the receptor for outside pathogens such as viruses.					
31541622	4	71	theme	antiviral	622:630	arg1	strategies					632:641	antiviral strategies	622:641	antiviral strategies	622:641	Identifying such glycoproteins is essential to understanding the mechanisms of viral infection, as well as developing antiviral strategies.					
31541622	0	72	theme	resistant	53:61	arg1	strains					79:85	viral resistant and susceptible strains	47:85	viral resistant and susceptible strains of Bombyx mori	47:100	Precision mapping of N- and O-glycoproteins in viral resistant and susceptible strains of Bombyx mori.					
31541622	6	73	dep	Bombyx	949:954	arg1	mori					956:959	Bombyx mori	949:959	two silkworm Bombyx mori strains 306 and NB	936:978	In this study, we performed a large-scale glycoproteomic survey for two silkworm Bombyx mori strains 306 and NB, which are susceptible and resistant to the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), respectively.					
31541622	10	74	theme	silkworm	1588:1595	arg1	strains					1597:1603	the two silkworm strains	1580:1603	the two silkworm strains	1580:1603	The variations in glycan site occupancy, as well as glycan diversity between the two silkworm strains, provide an insight into role of glycosylation in viral recognition and infection processes.					
31541622	7	75	theme	extensive	1218:1226	arg1	glycosylation					1228:1240	extensive glycosylation	1218:1240	extensive glycosylation	1218:1240	More than 400 silkworm N- and O- glycoproteins were identified with high confidence, demonstrating that this organism employs extensive glycosylation.					
31541622	3	76	theme	many	478:481	arg1	glycoproteins					489:501	many cases glycoproteins	478:501	many cases glycoproteins	478:501	During the initial stage of viral replication, viruses interact with cell membrane receptors, which are in many cases glycoproteins.					
31541622	4	77	theme	infection	589:597	arg1	mechanisms					569:578	the mechanisms	565:578	the mechanisms of viral infection	565:597	Identifying such glycoproteins is essential to understanding the mechanisms of viral infection, as well as developing antiviral strategies.					
29043680	5	0	theme	degradation	798:808	arg1	process					810:816	a highly conserved degradation process	779:816	a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis	779:907	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	6	1	theme	N-glycosylation	951:965	arg1	status					967:972	the N-glycosylation status	947:972	the N-glycosylation status	947:972	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
29043680	4	2	attach	released	508:515	arg2	glycoproteins					490:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	4	2	attach	released	508:515	arg1	processes					528:536	these processes	522:536	these processes	522:536	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	5	3	dep	process	810:816	arg1	maintain					885:892	maintain	885:892	maintain ER homeostasis	885:907	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	5	3	dep	process	810:816	arg1	prevent					821:827	prevent	821:827	prevent the accumulation or secretion of misfolded proteins	821:879	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	4	4	theme	further	595:601	arg1	processing					603:612	processing	603:612	processing	603:612	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	3	5	theme	carbohydrate	368:379	arg1	proteins					389:396	specific carbohydrate binding proteins	359:396	specific carbohydrate binding proteins	359:396	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	0	6	from	Analysis	0:7	arg1	ER					41:42	the ER	37:42	the ER	37:42	Analysis of Protein Glycosylation in the ER.					
29043680	1	7	theme	Protein	45:51	arg1	modification					103:114	an essential posttranslational modification	72:114	an essential posttranslational modification which is initiated in the endoplasmic reticulum	72:162	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	1	7	theme	Protein	45:51	arg1	N-glycosylation					53:67	Protein N-glycosylation	45:67	Protein N-glycosylation	45:67	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	3	8	theme	binding	381:387	arg1	proteins					389:396	specific carbohydrate binding proteins	359:396	specific carbohydrate binding proteins	359:396	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	3	9	theme	native	445:450	arg1	conformation					460:471	a native protein conformation	443:471	a native protein conformation	443:471	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	3	10	theme	specific	359:366	arg1	proteins					389:396	specific carbohydrate binding proteins	359:396	specific carbohydrate binding proteins	359:396	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	5	11	dep	accumulation	833:844	arg1	the					829:831	the	829:831	the	829:831	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	3	12	theme	protein	452:458	arg1	conformation					460:471	a native protein conformation	443:471	a native protein conformation	443:471	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	6	13	theme	degradation	1013:1023	arg1	process					1025:1031	the glycan-dependent ER-associated degradation process	978:1031	the glycan-dependent ER-associated degradation process in plants	978:1041	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
29043680	1	14	theme	essential	75:83	arg1	modification					103:114	an essential posttranslational modification	72:114	an essential posttranslational modification which is initiated in the endoplasmic reticulum	72:162	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	1	14	theme	essential	75:83	arg1	N-glycosylation					53:67	Protein N-glycosylation	45:67	Protein N-glycosylation	45:67	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	5	15	attach	removed	756:762	arg2	glycoproteins					738:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	5	15	attach	removed	756:762	arg1	ER					773:774	the ER	769:774	the ER	769:774	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	4	16	theme	different	697:705	arg1	functions					707:715	different functions	697:715	different functions	697:715	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	1	17	theme	posttranslational	85:101	arg1	modification					103:114	an essential posttranslational modification	72:114	an essential posttranslational modification which is initiated in the endoplasmic reticulum	72:162	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	1	17	theme	posttranslational	85:101	arg1	N-glycosylation					53:67	Protein N-glycosylation	45:67	Protein N-glycosylation	45:67	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	4	18	gly	glycoproteins	490:502	arg1	glycoproteins					490:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	0	19	theme	Glycosylation	20:32	arg1	Analysis					0:7	Analysis	0:7	Analysis of Protein Glycosylation in the ER.	0:43	Analysis of Protein Glycosylation in the ER.					
29043680	6	20	from	process	1025:1031	arg1	plants					1036:1041	plants	1036:1041	plants	1036:1041	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
29043680	5	21	theme	proteins	872:879	arg1	secretion					849:857	secretion	849:857	secretion	849:857	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	5	21	theme	proteins	872:879	arg1	accumulation					833:844	accumulation	833:844	accumulation	833:844	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	0	22	theme	Protein	12:18	arg1	Glycosylation					20:32	Protein Glycosylation	12:32	Protein Glycosylation	12:32	Analysis of Protein Glycosylation in the ER.					
29043680	3	23	theme	glycan	278:283	arg1	processing					285:294	glycan processing and binding reactions	278:316	processing	285:294	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	5	24	gly	glycoproteins	738:750	arg1	glycoproteins					738:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	3	25	theme	conformation	460:471	arg1	adoption					431:438	the adoption	427:438	the adoption of a native protein conformation	427:471	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	4	26	theme	structures	681:690	arg1	formation					655:663	the formation	651:663	the formation of more complex structures with different functions	651:715	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	6	27	theme	ER-associated	999:1011	arg1	process					1025:1031	the glycan-dependent ER-associated degradation process	978:1031	the glycan-dependent ER-associated degradation process in plants	978:1041	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
29043680	4	28	theme	folded	483:488	arg1	glycoproteins					490:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins	474:502	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	5	29	theme	ER	894:895	arg1	homeostasis					897:907	ER homeostasis	894:907	ER homeostasis	894:907	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	6	30	theme	glycan-dependent	982:997	arg1	process					1025:1031	the glycan-dependent ER-associated degradation process	978:1031	the glycan-dependent ER-associated degradation process in plants	978:1041	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
29043680	3	31	theme	binding	300:306	arg1	reactions					308:316	glycan processing and binding reactions	278:316	reactions	308:316	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	2	32	theme	protein	214:220	arg1	folding					222:228	protein folding	214:228	protein folding	214:228	In plants, the N-glycans play a pivotal role for protein folding and quality control.					
29043680	3	33	theme	reactions	308:316	arg1	interaction					263:273	the interaction	259:273	the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins	259:396	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	4	34	with	structures	681:690	arg1	functions					707:715	different functions	697:715	different functions	697:715	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	3	35	theme	processing	285:294	arg1	interaction					263:273	the interaction	259:273	the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins	259:396	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	2	36	theme	pivotal	197:203	arg1	role					205:208	a pivotal role	195:208	a pivotal role for protein folding and quality control	195:248	In plants, the N-glycans play a pivotal role for protein folding and quality control.					
29043680	4	37	theme	N-glycans	632:640	arg1	maturation					618:627	maturation	618:627	maturation	618:627	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	4	37	theme	N-glycans	632:640	arg1	processing					603:612	processing	603:612	processing	603:612	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	5	38	theme	folded	731:736	arg1	glycoproteins					738:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins	718:750	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	5	39	theme	misfolded	862:870	arg1	proteins					872:879	misfolded proteins	862:879	misfolded proteins	862:879	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	3	40	theme	ER-resident	330:340	arg1	glycosidases					342:353	ER-resident glycosidases	330:353	ER-resident glycosidases	330:353	Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation.					
29043680	1	41	theme	endoplasmic	142:152	arg1	reticulum					154:162	the endoplasmic reticulum	138:162	the endoplasmic reticulum	138:162	Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum.					
29043680	4	42	theme	complex	673:679	arg1	structures					681:690	more complex structures	668:690	more complex structures with different functions	668:715	Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions.					
29043680	5	43	theme	conserved	788:796	arg1	process					810:816	a highly conserved degradation process	779:816	a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis	779:907	Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis.					
29043680	2	44	theme	quality	234:240	arg1	control					242:248	quality control	234:248	quality control	234:248	In plants, the N-glycans play a pivotal role for protein folding and quality control.					
29043680	6	45	from	status	967:972	arg1	plants					1036:1041	plants	1036:1041	plants	1036:1041	Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.					
31669606	5	0	theme	light-induced	806:818	arg1	products					849:856	light-induced Tyr side-chain fragmentation products	806:856	light-induced Tyr side-chain fragmentation products	806:856	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	1	1	with	properties	223:232	arg1	FcγRIIIA					325:332	FcγRIIIA	325:332	FcγRIIIA	325:332	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	1	with	properties	223:232	arg1	IIIA					319:322	an Fc γ receptor IIIA	302:322	an Fc γ receptor IIIA (FcγRIIIA)	302:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	5	2	theme	side-chain	824:833	arg1	products					849:856	light-induced Tyr side-chain fragmentation products	806:856	light-induced Tyr side-chain fragmentation products	806:856	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	7	3	theme	Man5	1019:1022	arg1	glycoforms					1024:1033	The HM and Man5 glycoforms	1008:1033	The HM and Man5 glycoforms	1008:1033	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	2	4	theme	High	336:339	arg1	mannose					341:347	High mannose	336:347	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4])	336:379	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality.					
31669606	9	5	theme	such	1307:1310	arg1	glycoforms					1312:1321	such glycoforms	1307:1321	such glycoforms	1307:1321	Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored.					
31669606	2	6	theme	good	446:449	arg1	quality					451:457	good quality	446:457	good quality	446:457	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality.					
31669606	0	7	from	Effects	0:6	arg1	Stability					35:43	Stability	35:43	Stability	35:43	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	0	7	from	Effects	0:6	arg1	Binding					58:64	Receptor Binding	49:64	Receptor Binding	49:64	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	9	8	dep	need	1327:1330	arg1	Dependent					1247:1255	Dependent	1247:1255	Dependent	1247:1255	Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored.					
31669606	2	9	dep	mannose	341:347	arg1	GlcNAc2Man					354:363	GlcNAc2Man	354:363	GlcNAc2Man	354:363	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality.					
31669606	2	9	dep	mannose	341:347	arg1	HM					350:351	HM	350:351	HM	350:351	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality.					
31669606	5	10	theme	following	893:901	arg1	order					903:907	the following order	889:907	the following order	889:907	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	0	11	theme	IgG4-Fc	72:78	arg1	Stability					35:43	Stability	35:43	Stability	35:43	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	0	11	theme	IgG4-Fc	72:78	arg1	Binding					58:64	Receptor Binding	49:64	Receptor Binding	49:64	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	3	12	theme	physical	464:471	arg1	stability					473:481	The physical stability	460:481	The physical stability of these IgG4-Fc variants	460:507	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	1	13	used	utilized	143:150	arg2	series					83:88	A series	81:88	A series of well-defined N-glycosylated IgG4-Fc variants	81:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	10	14	theme	nonglycosylated	1363:1377	arg1	IgG4-Fc					1385:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc did not present measurable affinity to FcγRIIIA.					
31669606	5	15	theme	glycans	766:772	arg1	size					758:761	The size	754:761	The size of glycans at Asn297	754:782	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	5	16	theme	fragmentation	835:847	arg1	products					849:856	light-induced Tyr side-chain fragmentation products	806:856	light-induced Tyr side-chain fragmentation products	806:856	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	7	17	theme	HM	1012:1013	arg1	glycoforms					1024:1033	The HM and Man5 glycoforms	1008:1033	The HM and Man5 glycoforms	1008:1033	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	4	18	theme	HPLC-MS/MS	698:707	arg1	analysis					709:716	HPLC-MS/MS analysis	698:716	HPLC-MS/MS analysis of specific products	698:737	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	5	19	dep	yields	796:801	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	5	20	theme	>	926:926	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	6	21	theme	glycoforms	996:1005	arg1	stability					979:987	the thermal stability	967:987	the thermal stability of the glycoforms	967:1005	These yields correlate with the thermal stability of the glycoforms.					
31669606	8	22	gly	fucosylated	1229:1239	arg1	IgG1					1241:1244	fucosylated IgG1	1229:1244	fucosylated IgG1	1229:1244	The affinities measured for the HM and Man5 IgG4-Fc (0.39-0.52 μM) are similar to those measured for fucosylated IgG1.					
31669606	3	23	theme	IgG4-Fc	492:498	arg1	variants					500:507	these IgG4-Fc variants	486:507	these IgG4-Fc variants	486:507	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	6	24	theme	thermal	971:977	arg1	stability					979:987	the thermal stability	967:987	the thermal stability of the glycoforms	967:1005	These yields correlate with the thermal stability of the glycoforms.					
31669606	0	25	theme	Structure	18:26	arg1	Effects					0:6	Effects	0:6	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc	0:78	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	4	26	theme	max =	679:683	arg1	nm					689:690	λ max = 305 nm	677:690	λ max = 305 nm	677:690	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	4	26	theme	max =	679:683	arg1	nm					673:674	295 and 340 nm	661:674	nm	673:674	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	1	27	with	interactions	284:295	arg1	FcγRIIIA					325:332	FcγRIIIA	325:332	FcγRIIIA	325:332	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	27	with	interactions	284:295	arg1	IIIA					319:322	an Fc γ receptor IIIA	302:322	an Fc γ receptor IIIA (FcγRIIIA)	302:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	5	28	theme	>	933:933	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	1	29	theme	glycan	181:186	arg1	structure					188:196	glycan structure	181:196	glycan structure	181:196	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	0	30	theme	Glycan	11:16	arg1	Structure					18:26	Glycan Structure	11:26	Glycan Structure	11:26	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	4	31	theme	295	661:663	arg1	nm					689:690	λ max = 305 nm	677:690	λ max = 305 nm	677:690	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	4	31	theme	295	661:663	arg1	nm					673:674	295 and 340 nm	661:674	nm	673:674	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	1	32	theme	structure	188:196	arg1	effect					171:176	the effect	167:176	the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA)	167:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	7	33	theme	GlcNAc1	1111:1117	arg1	IgG4-Fc					1119:1125	GlcNAc1 IgG4-Fc	1111:1125	GlcNAc1 IgG4-Fc	1111:1125	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	4	34	theme	340	669:671	arg1	nm					689:690	λ max = 305 nm	677:690	λ max = 305 nm	677:690	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	4	34	theme	340	669:671	arg1	nm					673:674	295 and 340 nm	661:674	nm	673:674	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	5	35	theme	Man5	928:931	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	8	36	dep	HM	1160:1161	arg1	IgG4-Fc					1172:1178	IgG4-Fc	1172:1178	IgG4-Fc	1172:1178	The affinities measured for the HM and Man5 IgG4-Fc (0.39-0.52 μM) are similar to those measured for fucosylated IgG1.					
31669606	8	36	dep	HM	1160:1161	arg1	the					1156:1158	the	1156:1158	the	1156:1158	The affinities measured for the HM and Man5 IgG4-Fc (0.39-0.52 μM) are similar to those measured for fucosylated IgG1.					
31669606	1	37	theme	Fc	305:306	arg1	FcγRIIIA					325:332	FcγRIIIA	325:332	FcγRIIIA	325:332	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	37	theme	Fc	305:306	arg1	IIIA					319:322	an Fc γ receptor IIIA	302:322	an Fc γ receptor IIIA (FcγRIIIA)	302:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	38	gly	N-glycosylated	106:119	arg1	variants					129:136	well-defined N-glycosylated IgG4-Fc variants	93:136	well-defined N-glycosylated IgG4-Fc variants	93:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	3	39	theme	intrinsic	565:573	arg1	spectroscopy					588:599	intrinsic fluorescence spectroscopy	565:599	intrinsic fluorescence spectroscopy	565:599	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	0	40	dep	Stability	35:43	arg1	the					31:33	the	31:33	the	31:33	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	7	41	theme	increased	1043:1051	arg1	affinity					1053:1060	increased affinity	1043:1060	increased affinity for FcγRIIIA	1043:1073	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	1	42	theme	γ	308:308	arg1	FcγRIIIA					325:332	FcγRIIIA	325:332	FcγRIIIA	325:332	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	42	theme	γ	308:308	arg1	IIIA					319:322	an Fc γ receptor IIIA	302:322	an Fc γ receptor IIIA (FcγRIIIA)	302:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	7	43	gly	glycoforms	1024:1033	arg1	Man5					1019:1022	Man5	1019:1022	Man5	1019:1022	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	7	43	gly	glycoforms	1024:1033	arg1	HM					1012:1013	HM	1012:1013	HM	1012:1013	The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc.					
31669606	1	44	dep	properties	223:232	arg1	photostability					264:277	photostability	264:277	photostability	264:277	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	44	dep	properties	223:232	arg1	properties					223:232	their physicochemical properties	201:232	their physicochemical properties (conformational stability and photostability)	201:278	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	44	dep	properties	223:232	arg1	stability					250:258	conformational stability	235:258	conformational stability	235:258	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	3	45	theme	variants	500:507	arg1	stability					473:481	The physical stability	460:481	The physical stability of these IgG4-Fc variants	460:507	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	5	46	from	Asn297	777:782	arg1	size					758:761	The size	754:761	The size of glycans at Asn297	754:782	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	5	47	theme	products	849:856	arg1	yields					796:801	the yields	792:801	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	10	48	theme	N297Q	1379:1383	arg1	IgG4-Fc					1385:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc did not present measurable affinity to FcγRIIIA.					
31669606	1	49	theme	receptor	310:317	arg1	FcγRIIIA					325:332	FcγRIIIA	325:332	FcγRIIIA	325:332	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	49	theme	receptor	310:317	arg1	IIIA					319:322	an Fc γ receptor IIIA	302:322	an Fc γ receptor IIIA (FcγRIIIA)	302:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	3	50	theme	fluorescence	575:586	arg1	spectroscopy					588:599	intrinsic fluorescence spectroscopy	565:599	intrinsic fluorescence spectroscopy	565:599	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	1	51	theme	well-defined	93:104	arg1	variants					129:136	well-defined N-glycosylated IgG4-Fc variants	93:136	well-defined N-glycosylated IgG4-Fc variants	93:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	4	52	theme	products	730:737	arg1	analysis					709:716	HPLC-MS/MS analysis	698:716	HPLC-MS/MS analysis of specific products	698:737	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	10	53	theme	measurable	1409:1418	arg1	affinity					1420:1427	measurable affinity	1409:1427	measurable affinity to FcγRIIIA	1409:1439	The nonglycosylated N297Q IgG4-Fc did not present measurable affinity to FcγRIIIA.					
31669606	5	54	theme	>	916:916	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	4	55	theme	specific	721:728	arg1	products					730:737	specific products	721:737	specific products	721:737	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	1	56	theme	N-glycosylated	106:119	arg1	variants					129:136	well-defined N-glycosylated IgG4-Fc variants	93:136	well-defined N-glycosylated IgG4-Fc variants	93:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	9	57	theme	action	1278:1283	arg1	mechanisms					1264:1273	the mechanisms	1260:1273	the mechanisms of action of IgG4 therapeutics	1260:1304	Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored.					
31669606	1	58	theme	physicochemical	207:221	arg1	photostability					264:277	photostability	264:277	photostability	264:277	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	58	theme	physicochemical	207:221	arg1	properties					223:232	their physicochemical properties	201:232	their physicochemical properties (conformational stability and photostability)	201:278	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	58	theme	physicochemical	207:221	arg1	stability					250:258	conformational stability	235:258	conformational stability	235:258	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	5	59	theme	N297Q	910:914	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	5	60	theme	GlcNAc1	918:924	arg1	HM					935:936	N297Q > GlcNAc1 > Man5 > HM	910:936	the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM	792:936	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	1	61	from	effect	171:176	arg1	photostability					264:277	photostability	264:277	photostability	264:277	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	61	from	effect	171:176	arg1	properties					223:232	their physicochemical properties	201:232	their physicochemical properties (conformational stability and photostability)	201:278	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	61	from	effect	171:176	arg1	stability					250:258	conformational stability	235:258	conformational stability	235:258	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	61	from	effect	171:176	arg1	interactions					284:295	interactions	284:295	interactions with an Fc γ receptor IIIA (FcγRIIIA)	284:333	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	62	theme	IgG4-Fc	121:127	arg1	variants					129:136	well-defined N-glycosylated IgG4-Fc variants	93:136	well-defined N-glycosylated IgG4-Fc variants	93:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	8	63	theme	fucosylated	1229:1239	arg1	IgG1					1241:1244	fucosylated IgG1	1229:1244	fucosylated IgG1	1229:1244	The affinities measured for the HM and Man5 IgG4-Fc (0.39-0.52 μM) are similar to those measured for fucosylated IgG1.					
31669606	4	64	theme	λ	677:677	arg1	nm					689:690	λ max = 305 nm	677:690	λ max = 305 nm	677:690	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	4	64	theme	λ	677:677	arg1	nm					673:674	295 and 340 nm	661:674	nm	673:674	Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed.					
31669606	5	65	theme	Tyr	820:822	arg1	products					849:856	light-induced Tyr side-chain fragmentation products	806:856	light-induced Tyr side-chain fragmentation products	806:856	The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM.					
31669606	3	66	theme	differential	527:538	arg1	calorimetry					549:559	differential scanning calorimetry	527:559	differential scanning calorimetry	527:559	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	1	67	theme	variants	129:136	arg1	series					83:88	A series	81:88	A series of well-defined N-glycosylated IgG4-Fc variants	81:136	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	10	68	gly	nonglycosylated	1363:1377	arg1	IgG4-Fc					1385:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc	1359:1391	The nonglycosylated N297Q IgG4-Fc did not present measurable affinity to FcγRIIIA.					
31669606	9	69	theme	IgG4	1288:1291	arg1	therapeutics					1293:1304	IgG4 therapeutics	1288:1304	IgG4 therapeutics	1288:1304	Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored.					
31669606	0	70	theme	Receptor	49:56	arg1	Binding					58:64	Receptor Binding	49:64	Receptor Binding	49:64	Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc.					
31669606	3	71	theme	scanning	540:547	arg1	calorimetry					549:559	differential scanning calorimetry	527:559	differential scanning calorimetry	527:559	The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy.					
31669606	2	72	theme	N297Q	415:419	arg1	IgG4-Fc					421:427	N297Q IgG4-Fc	415:427	N297Q IgG4-Fc	415:427	High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality.					
31669606	9	73	theme	therapeutics	1293:1304	arg1	action					1278:1283	action	1278:1283	action of IgG4 therapeutics	1278:1304	Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored.					
31669606	1	74	theme	conformational	235:248	arg1	properties					223:232	their physicochemical properties	201:232	their physicochemical properties (conformational stability and photostability)	201:278	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
31669606	1	74	theme	conformational	235:248	arg1	stability					250:258	conformational stability	235:258	conformational stability	235:258	A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA).					
30136207	0	0	theme	silkworm	105:112	arg1	cells					119:123	cultured silkworm BmN4 cells	96:123	cultured silkworm BmN4 cells	96:123	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	5	1	theme	peptide	1154:1160	arg1	substrates					1174:1183	both peptide and protein substrates	1149:1183	substrates	1174:1183	Furthermore, a recombinant BmPGANT2 protein was successfully purified from silkworm-BEVS and exhibited a high ability to transfer GalNAc for both peptide and protein substrates.					
30136207	4	2	from	screenings	800:809	arg1	cells					837:841	cultured silkworm BmN4 cells	814:841	cultured silkworm BmN4 cells	814:841	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	1	3	theme	polypeptide	180:190	arg1	N-acetylgalactosaminyltransferases					192:225	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	169:225	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs)	169:249	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	0	4	theme	cultured	96:103	arg1	cells					119:123	cultured silkworm BmN4 cells	96:123	cultured silkworm BmN4 cells	96:123	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	6	5	theme	future	1327:1332	arg1	studies					1334:1340	future studies	1327:1340	future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans	1327:1446	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	0	6	from	O-glycosylation	77:91	arg1	cells					119:123	cultured silkworm BmN4 cells	96:123	cultured silkworm BmN4 cells	96:123	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	2	7	from	Lepidoptera	349:359	arg1	model					338:342	the insect model	327:342	the insect model from Lepidoptera	327:359	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	2	7	from	Lepidoptera	349:359	arg1	silkworm					362:369	silkworm	362:369	silkworm (Bombyx mori)	362:383	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	4	8	theme	unique	955:960	arg1	cell/tissue					962:972	unique cell/tissue or protein substrate preferences	955:1005	cell/tissue	962:972	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	5	9	theme	protein	1166:1172	arg1	substrates					1174:1183	both peptide and protein substrates	1149:1183	substrates	1174:1183	Furthermore, a recombinant BmPGANT2 protein was successfully purified from silkworm-BEVS and exhibited a high ability to transfer GalNAc for both peptide and protein substrates.					
30136207	4	10	theme	functional	894:903	arg1	BmPGANT					905:911	the solo functional BmPGANT	885:911	the solo functional BmPGANT for PRG4	885:920	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	4	10	theme	functional	894:903	arg1	Bmpgant2					873:880	Bmpgant2	873:880	Bmpgant2	873:880	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	0	11	gly	O-glycosylation	77:91	arg1	cells					119:123	cultured silkworm BmN4 cells	96:123	cultured silkworm BmN4 cells	96:123	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	2	12	theme	insect	331:336	arg1	model					338:342	the insect model	327:342	the insect model from Lepidoptera	327:359	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	2	12	theme	insect	331:336	arg1	silkworm					362:369	silkworm	362:369	silkworm (Bombyx mori)	362:383	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	0	13	theme	BmN4	114:117	arg1	cells					119:123	cultured silkworm BmN4 cells	96:123	cultured silkworm BmN4 cells	96:123	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	3	14	theme	proteoglycan	744:755	arg1	protein					766:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	4	15	theme	solo	889:892	arg1	BmPGANT					905:911	the solo functional BmPGANT	885:911	the solo functional BmPGANT for PRG4	885:920	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	4	15	theme	solo	889:892	arg1	Bmpgant2					873:880	Bmpgant2	873:880	Bmpgant2	873:880	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	3	16	theme	protein	515:521	arg1	analysis					539:546	protein O-glycosylation analysis	515:546	protein O-glycosylation analysis	515:546	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	6	17	with	pathways	1391:1398	arg1	O-glycans					1438:1446	O-glycans	1438:1446	O-glycans	1438:1446	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	1	18	theme	protein	305:311	arg1	substrate					313:321	a protein substrate	303:321	a protein substrate	303:321	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	6	19	theme	glycoproteins	1419:1431	arg1	pathways					1391:1398	the detailed silkworm O-glycosylation pathways	1353:1398	the detailed silkworm O-glycosylation pathways	1353:1398	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	6	19	theme	glycoproteins	1419:1431	arg1	productions					1404:1414	productions	1404:1414	productions of glycoproteins with O-glycans	1404:1446	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	6	20	theme	fundamental	1302:1312	arg1	insights					1314:1321	crucial fundamental insights	1294:1321	crucial fundamental insights	1294:1321	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	4	21	theme	functional	789:798	arg1	screenings					800:809	functional screenings	789:809	functional screenings in cultured silkworm BmN4 cells for nine Bmpgants	789:859	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	3	22	theme	baculovirus-produced	717:736	arg1	protein					766:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	6	23	theme	crucial	1294:1300	arg1	insights					1314:1321	crucial fundamental insights	1294:1321	crucial fundamental insights	1294:1321	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	6	24	with	productions	1404:1414	arg1	O-glycans					1438:1446	O-glycans	1438:1446	O-glycans	1438:1446	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	0	25	theme	functional	2:11	arg1	PGANT					60:64	PGANT	60:64	PGANT	60:64	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	0	25	theme	functional	2:11	arg1	N-acetylgalactosaminyltransferase					25:57	A functional polypeptide N-acetylgalactosaminyltransferase	0:57	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT)	0:65	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	3	26	theme	human	738:742	arg1	protein					766:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	2	27	dep	silkworm	362:369	arg1	mori					379:382	Bombyx mori	372:382	silkworm (Bombyx mori)	362:383	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	2	28	theme	O-glycosylation	395:409	arg1	pathway					411:417	O-glycosylation pathway	395:417	O-glycosylation pathway	395:417	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	1	29	theme	substrate	313:321	arg1	threonine					282:290	threonine	282:290	threonine	282:290	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	1	29	theme	substrate	313:321	arg1	serine					272:277	serine	272:277	serine	272:277	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	3	30	from	report	498:503	arg1	silkworms					551:559	silkworms	551:559	silkworms	551:559	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	1	31	dep	N-acetylgalactosaminyltransferases	192:225	arg1	PGANTs					243:248	PGANTs	243:248	PGANTs	243:248	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	1	31	dep	N-acetylgalactosaminyltransferases	192:225	arg1	ppGalNAc-Ts					228:238	ppGalNAc-Ts	228:238	ppGalNAc-Ts	228:238	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	4	32	theme	silkworm	823:830	arg1	cells					837:841	cultured silkworm BmN4 cells	814:841	cultured silkworm BmN4 cells	814:841	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	6	33	gly	glycoproteins	1419:1431	arg1	glycoproteins					1419:1431	glycoproteins	1419:1431	glycoproteins	1419:1431	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	5	34	theme	BmPGANT2	1035:1042	arg1	protein					1044:1050	a recombinant BmPGANT2 protein	1021:1050	a recombinant BmPGANT2 protein	1021:1050	Furthermore, a recombinant BmPGANT2 protein was successfully purified from silkworm-BEVS and exhibited a high ability to transfer GalNAc for both peptide and protein substrates.					
30136207	0	35	theme	polypeptide	13:23	arg1	PGANT					60:64	PGANT	60:64	PGANT	60:64	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	0	35	theme	polypeptide	13:23	arg1	N-acetylgalactosaminyltransferase					25:57	A functional polypeptide N-acetylgalactosaminyltransferase	0:57	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT)	0:65	A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.					
30136207	4	36	contain	have	950:953	arg2	preferences					995:1005	unique cell/tissue or protein substrate preferences	955:1005	preferences	995:1005	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	4	36	contain	have	950:953	arg1	Bmpgants					937:944	Bmpgants	937:944	Bmpgants	937:944	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	4	36	contain	have	950:953	arg2	cell/tissue					962:972	unique cell/tissue or protein substrate preferences	955:1005	cell/tissue	962:972	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	1	37	theme	Mucin-type	126:135	arg1	O-glycosylation					137:151	Mucin-type O-glycosylation	126:151	Mucin-type O-glycosylation	126:151	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	3	38	theme	PRG4	760:763	arg1	protein					766:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	a baculovirus-produced human proteoglycan 4 (PRG4) protein	715:772	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	6	39	from	BmPGANT2	1247:1254	arg1	cells					1277:1281	cultured silkworm cells	1259:1281	cultured silkworm cells	1259:1281	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	3	40	theme	core	609:612	arg1	branch					647:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch	600:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure	600:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	6	41	theme	O-glycosylation	1375:1389	arg1	pathways					1391:1398	the detailed silkworm O-glycosylation pathways	1353:1398	the detailed silkworm O-glycosylation pathways	1353:1398	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	3	42	theme	GalNAc	627:632	arg1	branch					647:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch	600:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure	600:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	4	43	theme	BmN4	832:835	arg1	cells					837:841	cultured silkworm BmN4 cells	814:841	cultured silkworm BmN4 cells	814:841	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	6	44	theme	silkworm	1366:1373	arg1	pathways					1391:1398	the detailed silkworm O-glycosylation pathways	1353:1398	the detailed silkworm O-glycosylation pathways	1353:1398	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	1	45	dep	serine	272:277	arg1	residue					292:298	residue	292:298	residue	292:298	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	6	46	theme	cultured	1259:1266	arg1	cells					1277:1281	cultured silkworm cells	1259:1281	cultured silkworm cells	1259:1281	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	2	47	theme	Bombyx	372:377	arg1	mori					379:382	Bombyx mori	372:382	silkworm (Bombyx mori)	362:383	In the insect model from Lepidoptera, silkworm (Bombyx mori), however, O-glycosylation pathway is totally unexplored and remains largely unknown.					
30136207	6	48	theme	present	1206:1212	arg1	results					1214:1220	the present results	1202:1220	the present results	1202:1220	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	6	49	theme	detailed	1357:1364	arg1	pathways					1391:1398	the detailed silkworm O-glycosylation pathways	1353:1398	the detailed silkworm O-glycosylation pathways	1353:1398	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	3	50	theme	disaccharide	634:645	arg1	branch					647:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch	600:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure	600:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	51	theme	common	602:607	arg1	branch					647:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch	600:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure	600:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	52	theme	O-glycosylation	523:537	arg1	analysis					539:546	protein O-glycosylation analysis	515:546	protein O-glycosylation analysis	515:546	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	5	53	theme	high	1113:1116	arg1	ability					1118:1124	a high ability	1111:1124	a high ability to transfer GalNAc for both peptide and protein substrates	1111:1183	Furthermore, a recombinant BmPGANT2 protein was successfully purified from silkworm-BEVS and exhibited a high ability to transfer GalNAc for both peptide and protein substrates.					
30136207	5	54	theme	recombinant	1023:1033	arg1	protein					1044:1050	a recombinant BmPGANT2 protein	1021:1050	a recombinant BmPGANT2 protein	1021:1050	Furthermore, a recombinant BmPGANT2 protein was successfully purified from silkworm-BEVS and exhibited a high ability to transfer GalNAc for both peptide and protein substrates.					
30136207	6	55	theme	silkworm	1268:1275	arg1	cells					1277:1281	cultured silkworm cells	1259:1281	cultured silkworm cells	1259:1281	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	6	56	theme	functional	1236:1245	arg1	BmPGANT2					1247:1254	the functional BmPGANT2	1232:1254	the functional BmPGANT2 in cultured silkworm cells	1232:1281	Taken together, the present results clarified the functional BmPGANT2 in cultured silkworm cells, providing crucial fundamental insights for future studies dissecting the detailed silkworm O-glycosylation pathways and productions of glycoproteins with O-glycans.					
30136207	3	57	theme	first	492:496	arg1	report					498:503	the first report	488:503	the first report regarding protein O-glycosylation analysis in silkworms	488:559	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	57	theme	first	492:496	arg1	profile					587:593	the O-glycan profile	574:593	the O-glycan profile	574:593	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	58	theme	O-glycan	578:585	arg1	report					498:503	the first report	488:503	the first report regarding protein O-glycosylation analysis in silkworms	488:559	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	58	theme	O-glycan	578:585	arg1	profile					587:593	the O-glycan profile	574:593	the O-glycan profile	574:593	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	4	59	theme	substrate	985:993	arg1	preferences					995:1005	unique cell/tissue or protein substrate preferences	955:1005	preferences	995:1005	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	3	60	theme	sialylated	673:682	arg1	structure					684:692	terminally sialylated structure	662:692	terminally sialylated structure	662:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	3	61	theme	Gal	616:618	arg1	branch					647:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch	600:652	a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure	600:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	1	62	theme	UDP-GalNAc	169:178	arg1	N-acetylgalactosaminyltransferases					192:225	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	169:225	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs)	169:249	Mucin-type O-glycosylation is initiated by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts or PGANTs), attaching GalNAc to serine or threonine residue of a protein substrate.					
30136207	3	63	gly	sialylated	673:682	arg1	structure					684:692	terminally sialylated structure	662:692	terminally sialylated structure	662:692	In this study, as the first report regarding protein O-glycosylation analysis in silkworms, we verified the O-glycan profile that a common core 1 Gal (β1-3) GalNAc disaccharide branch without terminally sialylated structure is mainly formed for a baculovirus-produced human proteoglycan 4 (PRG4) protein.					
30136207	4	64	theme	protein	977:983	arg1	preferences					995:1005	unique cell/tissue or protein substrate preferences	955:1005	preferences	995:1005	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
30136207	4	65	theme	cultured	814:821	arg1	cells					837:841	cultured silkworm BmN4 cells	814:841	cultured silkworm BmN4 cells	814:841	Intriguingly, functional screenings in cultured silkworm BmN4 cells for nine Bmpgants reveal that Bmpgant2 is the solo functional BmPGANT for PRG4, implying that Bmpgants may have unique cell/tissue or protein substrate preferences.					
29888905	10	0	from	field	1901:1905	arg1	glioma					1910:1915	glioma	1910:1915	glioma	1910:1915	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	3	1	theme	glioma	642:647	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	1	theme	glioma	642:647	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	1	2	theme	bodily	221:226	arg1	fluids					228:233	bodily fluids	221:233	bodily fluids	221:233	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	9	3	theme	proximally	1672:1681	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	3	4	from	strategy	467:474	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	4	from	strategy	467:474	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	4	from	strategy	467:474	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	4	from	strategy	467:474	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	7	5	theme	mannose	1328:1334	arg1	glycans					1336:1342	high mannose glycans	1323:1342	high mannose glycans	1323:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	9	6	with	heterogeneous	1622:1634	arg1	tri-					1647:1650	tri-	1647:1650	tri-	1647:1650	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	6	with	heterogeneous	1622:1634	arg1	di-					1642:1644	di-	1642:1644	di-	1642:1644	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	6	with	heterogeneous	1622:1634	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	3	7	theme	quantitative	496:507	arg1	profiling					509:517	high-resolution quantitative profiling	480:517	high-resolution quantitative profiling	480:517	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	2	8	theme	Many	282:285	arg1	studies					287:293	Many studies	282:293	Many studies on EV nucleic acid, lipid, and protein composition	282:344	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	10	9	theme	glycans	1828:1834	arg1	roles					1819:1823	the roles	1815:1823	the roles of glycans in EVs biological properties	1815:1863	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	8	10	gly	fucosylated	1481:1491	arg1	N-glycan					1442:1449	the predominant N-glycan	1426:1449	the predominant N-glycan	1426:1449	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	9	11	theme	Galα3Gal	1734:1741	arg1	structure					1743:1751	peripheral Galα3Gal structure	1723:1751	peripheral Galα3Gal structure	1723:1751	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	8	12	theme	predominant	1430:1440	arg1	N-glycan					1442:1449	the predominant N-glycan	1426:1449	the predominant N-glycan	1426:1449	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	7	13	theme	mannose	1386:1392	arg1	glycans					1394:1400	high mannose glycans	1381:1400	high mannose glycans	1381:1400	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	10	14	theme	EVs	1839:1841	arg1	properties					1854:1863	EVs biological properties	1839:1863	EVs biological properties	1839:1863	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	3	15	theme	EV	563:564	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	15	theme	EV	563:564	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	15	theme	EV	563:564	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	15	theme	EV	563:564	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	5	16	theme	electron	872:879	arg1	microscopy					881:890	electron microscopy	872:890	electron microscopy	872:890	CMs and EVs have been characterized by immunoblotting using a panel of EV-specific antibodies, electron microscopy, and immunocytochemistry.					
29888905	7	17	from	enrichment	1241:1250	arg1	N-glycans					1263:1271	complex N-glycans	1255:1271	complex N-glycans	1255:1271	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	6	18	with	glycopeptides	972:984	arg1	F					1013:1013	peptide N-glycosidase F	991:1013	peptide N-glycosidase F	991:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	19	from	elucidation	533:543	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	19	from	elucidation	533:543	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	19	from	elucidation	533:543	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	19	from	elucidation	533:543	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	1	20	theme	extracellular	126:138	arg1	surroundings					108:119	the surroundings	104:119	the surroundings	104:119	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	1	20	theme	extracellular	126:138	arg1	EVs					150:152	EVs	150:152	EVs	150:152	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	1	20	theme	extracellular	126:138	arg1	vesicles					140:147	the extracellular vesicles	122:147	the extracellular vesicles (EVs)	122:153	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	7	21	theme	complex	1255:1261	arg1	N-glycans					1263:1271	complex N-glycans	1255:1271	complex N-glycans	1255:1271	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	6	22	attach	released	933:940	arg2	N-Glycans					918:926	N-Glycans	918:926	N-Glycans	918:926	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	22	attach	released	933:940	arg1	glycopeptides					972:984	membrane-derived tryptic glycopeptides	947:984	membrane-derived tryptic glycopeptides with peptide N-glycosidase F	947:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	23	theme	N-glycosidase	999:1011	arg1	F					1013:1013	peptide N-glycosidase F	991:1013	peptide N-glycosidase F	991:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	8	24	theme	N-acetylneuraminic	1510:1527	arg1	acid					1529:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	5	25	theme	antibodies	860:869	arg1	panel					839:843	a panel	837:843	a panel of EV-specific antibodies	837:869	CMs and EVs have been characterized by immunoblotting using a panel of EV-specific antibodies, electron microscopy, and immunocytochemistry.					
29888905	2	26	from	studies	287:293	arg1	lipid					315:319	lipid	315:319	lipid	315:319	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	2	26	from	studies	287:293	arg1	acid					309:312	EV nucleic acid	298:312	EV nucleic acid	298:312	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	2	26	from	studies	287:293	arg1	composition					334:344	protein composition	326:344	protein composition	326:344	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	0	27	from	N-Glycosylation	0:14	arg1	HEK-293					47:53	HEK-293	47:53	HEK-293	47:53	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	0	27	from	N-Glycosylation	0:14	arg1	Lines					71:75	Glioma Cell Lines	59:75	Glioma Cell Lines	59:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	6	28	theme	tryptic	964:970	arg1	glycopeptides					972:984	membrane-derived tryptic glycopeptides	947:984	membrane-derived tryptic glycopeptides with peptide N-glycosidase F	947:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	4	29	theme	total	745:749	arg1	CMs					771:773	CMs	771:773	CMs	771:773	EVs have been purified from cell supernatants by ultracentrifugation and compared with total cellular membranes (CMs).					
29888905	4	29	theme	total	745:749	arg1	membranes					760:768	total cellular membranes	745:768	total cellular membranes (CMs)	745:774	EVs have been purified from cell supernatants by ultracentrifugation and compared with total cellular membranes (CMs).					
29888905	9	30	theme	Mouse	1586:1590	arg1	profiles					1603:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	6	31	theme	phase-high-pressure	1069:1087	arg1	NP-HPLC					1112:1118	NP-HPLC	1112:1118	NP-HPLC	1112:1118	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	31	theme	phase-high-pressure	1069:1087	arg1	chromatography					1096:1109	normal phase-high-pressure liquid chromatography	1062:1109	normal phase-high-pressure liquid chromatography (NP-HPLC)	1062:1119	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	2	32	theme	glycosylation	407:419	arg1	characterization					379:394	detailed characterization	370:394	detailed characterization of protein glycosylation	370:419	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	8	33	dep	proximally	1470:1479	arg1	tetraantennary					1455:1468	tetraantennary	1455:1468	tetraantennary	1455:1468	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	9	34	theme	EV	1600:1601	arg1	profiles					1603:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	7	35	theme	glycans	1336:1342	arg1	glycans					1336:1342	high mannose glycans	1323:1342	high mannose glycans	1323:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	7	35	theme	glycans	1336:1342	arg1	amount					1313:1318	a small amount	1305:1318	a small amount of high mannose glycans	1305:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	8	36	theme	LacdiNAc	1566:1573	arg1	structure					1575:1583	the LacdiNAc structure	1562:1583	the LacdiNAc structure	1562:1583	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	2	37	theme	detailed	370:377	arg1	characterization					379:394	detailed characterization	370:394	detailed characterization of protein glycosylation	370:419	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	0	38	theme	Extracellular	19:31	arg1	Vesicles					33:40	Extracellular Vesicles	19:40	Extracellular Vesicles from HEK-293 and Glioma Cell Lines	19:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	1	39	theme	emerging	254:261	arg1	targets					273:279	emerging biomarker targets	254:279	emerging biomarker targets	254:279	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	7	40	theme	high	1323:1326	arg1	glycans					1336:1342	high mannose glycans	1323:1342	high mannose glycans	1323:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	3	41	theme	human	616:620	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	41	theme	human	616:620	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	0	42	theme	Cell	66:69	arg1	Lines					71:75	Glioma Cell Lines	59:75	Glioma Cell Lines	59:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	10	43	theme	biomarker	1891:1899	arg1	field					1901:1905	the biomarker field	1887:1905	the biomarker field in glioma	1887:1915	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	6	44	theme	laser	1141:1145	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	45	theme	cell	589:592	arg1	lines					594:598	three cell lines	583:598	three cell lines	583:598	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	46	theme	mouse	636:640	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	46	theme	mouse	636:640	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	6	47	gly	glycopeptides	972:984	arg2	glycopeptides					972:984	membrane-derived tryptic glycopeptides	947:984	membrane-derived tryptic glycopeptides with peptide N-glycosidase F	947:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	2	48	theme	EV	298:299	arg1	acid					309:312	EV nucleic acid	298:312	EV nucleic acid	298:312	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	10	49	theme	novel	1773:1777	arg1	perspectives					1779:1790	novel perspectives	1773:1790	novel perspectives	1773:1790	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	7	50	theme	concomitant	1290:1300	arg1	EVs					1286:1288	EVs	1286:1288	EVs concomitant to a small amount of high mannose glycans	1286:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	9	51	theme	tetraantennary	1657:1670	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	52	theme	fucosylated	1683:1693	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	8	53	contain	contained	1552:1560	arg2	structure					1575:1583	the LacdiNAc structure	1562:1583	the LacdiNAc structure	1562:1583	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	8	53	contain	contained	1552:1560	arg1	EVs					1543:1545	HEK-293 EVs	1535:1545	HEK-293 EVs	1535:1545	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	3	54	theme	high-resolution	480:494	arg1	profiling					509:517	high-resolution quantitative profiling	480:517	high-resolution quantitative profiling	480:517	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	6	55	theme	mass	1196:1199	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	56	dep	glycoproteins	566:578	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	56	dep	glycoproteins	566:578	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	56	dep	glycoproteins	566:578	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	56	dep	glycoproteins	566:578	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	6	57	theme	membrane-derived	947:962	arg1	glycopeptides					972:984	membrane-derived tryptic glycopeptides	947:984	membrane-derived tryptic glycopeptides with peptide N-glycosidase F	947:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	58	theme	MALDI-TOF	1185:1193	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	59	theme	structure	523:531	arg1	elucidation					533:543	structure elucidation	523:543	structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	523:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	60	theme	N-glycans	548:556	arg1	elucidation					533:543	structure elucidation	523:543	structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	523:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	60	theme	N-glycans	548:556	arg1	strategy					467:474	a strategy	465:474	a strategy for high-resolution quantitative profiling	465:517	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	9	61	theme	peripheral	1723:1732	arg1	structure					1743:1751	peripheral Galα3Gal structure	1723:1751	peripheral Galα3Gal structure	1723:1751	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	6	62	theme	ionization-time-of-flight	1158:1182	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	63	gly	glycoproteins	566:578	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	63	gly	glycoproteins	566:578	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	63	gly	glycoproteins	566:578	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	63	gly	glycoproteins	566:578	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	10	64	theme	biological	1843:1852	arg1	properties					1854:1863	EVs biological properties	1839:1863	EVs biological properties	1839:1863	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	1	65	theme	other	191:195	arg1	cells					197:201	other cells	191:201	other cells	191:201	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	9	66	theme	structure	1743:1751	arg1	tri-					1647:1650	tri-	1647:1650	tri-	1647:1650	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	66	theme	structure	1743:1751	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	66	theme	structure	1743:1751	arg1	di-					1642:1644	di-	1642:1644	di-	1642:1644	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	9	66	theme	structure	1743:1751	arg1	presence					1711:1718	the presence	1707:1718	the presence of peripheral Galα3Gal structure	1707:1751	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	0	67	from	Lines	71:75	arg1	N-Glycosylation					0:14	N-Glycosylation	0:14	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.	0:76	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	0	67	from	Lines	71:75	arg1	Vesicles					33:40	Extracellular Vesicles	19:40	Extracellular Vesicles from HEK-293 and Glioma Cell Lines	19:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	6	68	link	membrane-derived	947:962	arg1	glycopeptides					972:984	membrane-derived tryptic glycopeptides	947:984	membrane-derived tryptic glycopeptides with peptide N-glycosidase F	947:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	7	69	theme	high	1381:1384	arg1	glycans					1394:1400	high mannose glycans	1381:1400	high mannose glycans	1381:1400	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	3	70	theme	lines	594:598	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	70	theme	lines	594:598	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	70	theme	lines	594:598	arg1	Tu-2449					649:655	mouse glioma Tu-2449	636:655	mouse glioma Tu-2449	636:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	70	theme	lines	594:598	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	8	71	theme	α2,3-linked	1498:1508	arg1	acid					1529:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	7	72	located	found	1277:1281	arg2	enrichment					1241:1250	enrichment	1241:1250	enrichment in complex N-glycans	1241:1271	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	7	72	located	found	1277:1281	arg1	EVs					1286:1288	EVs	1286:1288	EVs concomitant to a small amount of high mannose glycans	1286:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	5	73	theme	EV-specific	848:858	arg1	antibodies					860:869	EV-specific antibodies	848:869	EV-specific antibodies	848:869	CMs and EVs have been characterized by immunoblotting using a panel of EV-specific antibodies, electron microscopy, and immunocytochemistry.					
29888905	6	74	theme	peptide	991:997	arg1	F					1013:1013	peptide N-glycosidase F	991:1013	peptide N-glycosidase F	991:1013	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	8	75	theme	HEK-293	1535:1541	arg1	EVs					1543:1545	HEK-293 EVs	1535:1545	HEK-293 EVs	1535:1545	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	7	76	theme	cell	1229:1232	arg1	lines					1234:1238	the three cell lines	1219:1238	the three cell lines	1219:1238	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	4	77	theme	cellular	751:758	arg1	CMs					771:773	CMs	771:773	CMs	771:773	EVs have been purified from cell supernatants by ultracentrifugation and compared with total cellular membranes (CMs).					
29888905	4	77	theme	cellular	751:758	arg1	membranes					760:768	total cellular membranes	745:768	total cellular membranes (CMs)	745:774	EVs have been purified from cell supernatants by ultracentrifugation and compared with total cellular membranes (CMs).					
29888905	10	78	from	roles	1819:1823	arg1	properties					1854:1863	EVs biological properties	1839:1863	EVs biological properties	1839:1863	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	6	79	theme	liquid	1089:1094	arg1	NP-HPLC					1112:1118	NP-HPLC	1112:1118	NP-HPLC	1112:1118	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	79	theme	liquid	1089:1094	arg1	chromatography					1096:1109	normal phase-high-pressure liquid chromatography	1062:1109	normal phase-high-pressure liquid chromatography (NP-HPLC)	1062:1119	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	0	80	from	HEK-293	47:53	arg1	N-Glycosylation					0:14	N-Glycosylation	0:14	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.	0:76	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	0	80	from	HEK-293	47:53	arg1	Vesicles					33:40	Extracellular Vesicles	19:40	Extracellular Vesicles from HEK-293 and Glioma Cell Lines	19:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	9	81	theme	Tu-2449	1592:1598	arg1	profiles					1603:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles	1586:1610	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
29888905	7	82	from	enriched	1369:1376	arg1	glycans					1394:1400	high mannose glycans	1381:1400	high mannose glycans	1381:1400	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	6	83	theme	normal	1062:1067	arg1	NP-HPLC					1112:1118	NP-HPLC	1112:1118	NP-HPLC	1112:1118	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	6	83	theme	normal	1062:1067	arg1	chromatography					1096:1109	normal phase-high-pressure liquid chromatography	1062:1109	normal phase-high-pressure liquid chromatography (NP-HPLC)	1062:1119	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	2	84	theme	protein	399:405	arg1	glycosylation					407:419	protein glycosylation	399:419	protein glycosylation	399:419	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	4	85	theme	cell	686:689	arg1	supernatants					691:702	cell supernatants	686:702	cell supernatants	686:702	EVs have been purified from cell supernatants by ultracentrifugation and compared with total cellular membranes (CMs).					
29888905	2	86	theme	protein	326:332	arg1	composition					334:344	protein composition	326:344	protein composition	326:344	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	0	87	theme	Vesicles	33:40	arg1	N-Glycosylation					0:14	N-Glycosylation	0:14	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.	0:76	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	7	88	dep	found	1277:1281	arg1	whereas					1345:1351	whereas	1345:1351	whereas	1345:1351	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	8	89	link	α2,3-linked	1498:1508	arg1	acid					1529:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	α2,3-linked N-acetylneuraminic acid	1498:1532	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	10	90	dep	opened	1766:1771	arg1	investigate					1803:1813	investigate	1803:1813	to further investigate the roles of glycans in EVs biological properties	1792:1863	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	10	90	dep	opened	1766:1771	arg1	contribute					1873:1882	contribute	1873:1882	may contribute to the biomarker field in glioma	1869:1915	The results opened novel perspectives to further investigate the roles of glycans in EVs biological properties and may contribute to the biomarker field in glioma.					
29888905	3	91	from	glycoproteins	566:578	arg1	elucidation					533:543	structure elucidation	523:543	structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	523:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	91	from	glycoproteins	566:578	arg1	strategy					467:474	a strategy	465:474	a strategy for high-resolution quantitative profiling	465:517	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	91	from	glycoproteins	566:578	arg1	N-glycans					548:556	N-glycans	548:556	N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	548:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	1	92	theme	biomarker	263:271	arg1	targets					273:279	emerging biomarker targets	254:279	emerging biomarker targets	254:279	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	0	93	gly	N-Glycosylation	0:14	arg1	Vesicles					33:40	Extracellular Vesicles	19:40	Extracellular Vesicles from HEK-293 and Glioma Cell Lines	19:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	7	94	from	glycans	1394:1400	arg1	enriched					1369:1376	enriched	1369:1376	enriched	1369:1376	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	7	95	theme	small	1307:1311	arg1	glycans					1336:1342	high mannose glycans	1323:1342	high mannose glycans	1323:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	7	95	theme	small	1307:1311	arg1	amount					1313:1318	a small amount	1305:1318	a small amount of high mannose glycans	1305:1342	For the three cell lines, enrichment in complex N-glycans was found in EVs concomitant to a small amount of high mannose glycans, whereas CMs were highly enriched in high mannose glycans.					
29888905	3	96	theme	human	601:605	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	96	theme	human	601:605	arg1	HEK-293					607:613	human HEK-293	601:613	human HEK-293	601:613	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	0	97	theme	Glioma	59:64	arg1	Lines					71:75	Glioma Cell Lines	59:75	Glioma Cell Lines	59:75	N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines.					
29888905	1	98	located	found	212:216	arg2	Cells					78:82	Cells	78:82	Cells	78:82	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	1	98	located	found	212:216	arg1	fluids					228:233	bodily fluids	221:233	bodily fluids	221:233	Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets.					
29888905	6	99	theme	desorption	1147:1156	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	8	100	theme	HEK-293	1406:1412	arg1	EVs					1421:1423	HEK-293 and H4 EVs	1406:1423	HEK-293 and H4 EVs	1406:1423	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	2	101	theme	nucleic	301:307	arg1	acid					309:312	EV nucleic acid	298:312	EV nucleic acid	298:312	Many studies on EV nucleic acid, lipid, and protein composition are available; however, detailed characterization of protein glycosylation has been less approached.					
29888905	6	102	theme	matrix-assisted	1125:1139	arg1	spectrometry					1201:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry	1125:1212	N-Glycans were released from membrane-derived tryptic glycopeptides with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by normal phase-high-pressure liquid chromatography (NP-HPLC) and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.					
29888905	3	103	theme	glioma	622:627	arg1	glycoproteins					566:578	EV glycoproteins	563:578	EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449	563:655	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	3	103	theme	glioma	622:627	arg1	H4					629:630	human glioma H4	616:630	human glioma H4	616:630	Here, we describe a strategy for high-resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449.					
29888905	8	104	theme	H4	1418:1419	arg1	EVs					1421:1423	HEK-293 and H4 EVs	1406:1423	HEK-293 and H4 EVs	1406:1423	In HEK-293 and H4 EVs, the predominant N-glycan was tetraantennary proximally fucosylated with α2,3-linked N-acetylneuraminic acid; HEK-293 EVs also contained the LacdiNAc structure.					
29888905	9	105	gly	fucosylated	1683:1693	arg1	glycans					1695:1701	tetraantennary proximally fucosylated glycans	1657:1701	tetraantennary proximally fucosylated glycans	1657:1701	Mouse Tu-2449 EV profiles were very heterogeneous, with di-, tri-, and tetraantennary proximally fucosylated glycans and the presence of peripheral Galα3Gal structure.					
31838284	6	0	theme	notable	1007:1013	arg1	differences					1015:1025	notable differences	1007:1025	notable differences in N- and O-glycosylation	1007:1051	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	4	1	used	used	550:553	arg2	combination					480:490	a combination	478:490	a combination of chromatography and mass spectrometry analysis	478:539	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	2	theme	epoetin	669:675	arg1	NeoRecormon®					655:666	NeoRecormon®	655:666	NeoRecormon® (epoetin beta)	655:681	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	2	theme	epoetin	669:675	arg1	beta					677:680	epoetin beta	669:680	epoetin beta	669:680	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	3	theme	chromatography	495:508	arg1	analysis					532:539	chromatography and mass spectrometry analysis	495:539	chromatography and mass spectrometry analysis	495:539	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	4	gly	glycosylation	587:599	arg1	products					622:629	five ESA products	613:629	five ESA products	613:629	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	6	5	theme	sialic	1083:1088	arg1	acid					1090:1093	sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans	1083:1199	acid	1090:1093	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	3	6	theme	quality	364:370	arg1	Glycosylation					336:348	Glycosylation	336:348	Glycosylation	336:348	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	3	6	theme	quality	364:370	arg1	attribute					372:380	a critical quality attribute	353:380	a critical quality attribute of ESA products	353:396	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	4	7	theme	darbepoetin	768:778	arg1	Aranesp®					758:765	Aranesp®	758:765	Aranesp® (darbepoetin alfa)	758:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	7	theme	darbepoetin	768:778	arg1	alfa					780:783	darbepoetin alfa	768:783	darbepoetin alfa	768:783	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	7	8	theme	glycosylation	1343:1355	arg1	monitoring					1317:1326	continued monitoring	1307:1326	continued monitoring of ESA product glycosylation	1307:1355	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	4	9	theme	epoetin	640:646	arg1	Eprex®					632:637	Eprex®	632:637	Eprex® (epoetin alfa)	632:652	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	9	theme	epoetin	640:646	arg1	alfa					648:651	epoetin alfa	640:651	epoetin alfa	640:651	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	10	theme	epoetin	695:701	arg1	biosimilar					708:717	epoetin alfa biosimilar	695:717	epoetin alfa biosimilar	695:717	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	10	theme	epoetin	695:701	arg1	Binocrit®					684:692	Binocrit®	684:692	Binocrit® (epoetin alfa biosimilar)	684:718	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	7	11	theme	ESA	1507:1509	arg1	structure-activity-relationships					1511:1542	ESA structure-activity-relationships	1507:1542	ESA structure-activity-relationships	1507:1542	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	6	12	from	differences	1015:1025	arg1	O-glycosylation					1037:1051	O-glycosylation	1037:1051	O-glycosylation	1037:1051	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	12	from	differences	1015:1025	arg1	N-					1030:1031	N-	1030:1031	N-	1030:1031	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	13	theme	extended	1142:1149	arg1	antennae					1151:1158	N-acetyllactosamine extended antennae	1122:1158	N-acetyllactosamine extended antennae	1122:1158	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	3	14	contain	has	405:407	arg1	it					402:403	it	402:403	it	402:403	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	3	14	contain	has	405:407	arg2	influence					419:427	a crucial influence	409:427	a crucial influence	409:427	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	1	15	theme	chronic	168:174	arg1	disease					183:189	chronic kidney disease	168:189	chronic kidney disease	168:189	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	3	16	theme	critical	355:362	arg1	Glycosylation					336:348	Glycosylation	336:348	Glycosylation	336:348	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	3	16	theme	critical	355:362	arg1	attribute					372:380	a critical quality attribute	353:380	a critical quality attribute of ESA products	353:396	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	5	17	theme	O-glycan	959:966	arg1	characterisation					968:983	O-glycan characterisation	959:983	O-glycan characterisation	959:983	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	1	18	theme	kidney	176:181	arg1	disease					183:189	chronic kidney disease	168:189	chronic kidney disease	168:189	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	3	19	theme	products	389:396	arg1	Glycosylation					336:348	Glycosylation	336:348	Glycosylation	336:348	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	3	19	theme	products	389:396	arg1	attribute					372:380	a critical quality attribute	353:380	a critical quality attribute of ESA products	353:396	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	2	20	theme	second-generation	307:323	arg1	medicines					325:333	second-generation medicines	307:333	second-generation medicines	307:333	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	7	21	theme	structure-activity-relationships	1511:1542	arg1	understanding					1490:1502	our understanding	1486:1502	our understanding of ESA structure-activity-relationships	1486:1542	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	3	22	theme	crucial	411:417	arg1	influence					419:427	a crucial influence	409:427	a crucial influence	409:427	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	0	23	theme	Glycan	0:5	arg1	analysis					7:14	Glycan analysis	0:14	Glycan analysis of erythropoiesis-stimulating agents	0:51	Glycan analysis of erythropoiesis-stimulating agents.					
31838284	6	24	theme	sulphated/penta-sialylated	1164:1189	arg1	N-glycans					1191:1199	sulphated/penta-sialylated N-glycans	1164:1199	sulphated/penta-sialylated N-glycans	1164:1199	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	5	25	theme	mixed-mode	816:825	arg1	chromatography					866:879	mixed-mode anion-exchange/hydrophilic interaction chromatography	816:879	mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation	816:983	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	5	25	theme	mixed-mode	816:825	arg1	AEX/HILIC-MS					882:893	AEX/HILIC-MS	882:893	AEX/HILIC-MS	882:893	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	0	26	theme	erythropoiesis-stimulating	19:44	arg1	agents					46:51	erythropoiesis-stimulating agents	19:51	erythropoiesis-stimulating agents	19:51	Glycan analysis of erythropoiesis-stimulating agents.					
31838284	7	27	theme	pharmacological	1376:1390	arg1	data					1392:1395	pharmacological data	1376:1395	pharmacological data	1376:1395	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	1	28	gly	glycoproteins	122:134	arg1	glycoproteins					122:134	therapeutic glycoproteins	110:134	therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	110:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	7	29	dep	highlights	1283:1292	arg1	allied					1366:1371	allied	1366:1371	allied	1366:1371	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	2	30	from	available	238:246	arg1	Union					264:268	the European Union	251:268	the European Union	251:268	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	4	31	theme	products	622:629	arg1	Binocrit®					684:692	Binocrit®	684:692	Binocrit® (epoetin alfa biosimilar)	684:718	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	31	theme	products	622:629	arg1	Eprex®					632:637	Eprex®	632:637	Eprex® (epoetin alfa)	632:652	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	31	theme	products	622:629	arg1	Silapo					721:726	Silapo	721:726	Silapo (epoetin alfa biosimilar)	721:752	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	31	theme	products	622:629	arg1	NeoRecormon®					655:666	NeoRecormon®	655:666	NeoRecormon® (epoetin beta)	655:681	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	31	theme	products	622:629	arg1	Aranesp®					758:765	Aranesp®	758:765	Aranesp® (darbepoetin alfa)	758:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	31	theme	products	622:629	arg1	profiles					601:608	the glycosylation profiles	583:608	the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa)	583:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	3	32	theme	ESA	385:387	arg1	products					389:396	ESA products	385:396	ESA products	385:396	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	4	33	theme	alfa	703:706	arg1	biosimilar					708:717	epoetin alfa biosimilar	695:717	epoetin alfa biosimilar	695:717	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	33	theme	alfa	703:706	arg1	Binocrit®					684:692	Binocrit®	684:692	Binocrit® (epoetin alfa biosimilar)	684:718	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	34	theme	ESA	618:620	arg1	products					622:629	five ESA products	613:629	five ESA products	613:629	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	6	35	theme	N-acetyllactosamine	1122:1140	arg1	antennae					1151:1158	N-acetyllactosamine extended antennae	1122:1158	N-acetyllactosamine extended antennae	1122:1158	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	36	theme	therapeutic	1250:1260	arg1	potencies					1262:1270	therapeutic potencies	1250:1270	therapeutic potencies	1250:1270	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	37	dep	acid	1090:1093	arg1	occupation					1095:1104	occupation	1095:1104	occupation	1095:1104	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	0	38	theme	agents	46:51	arg1	analysis					7:14	Glycan analysis	0:14	Glycan analysis of erythropoiesis-stimulating agents	0:51	Glycan analysis of erythropoiesis-stimulating agents.					
31838284	4	39	theme	mass	514:517	arg1	spectrometry					519:530	mass spectrometry	514:530	mass spectrometry	514:530	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	2	40	theme	products	225:232	arg1	products					225:232	ESA products	221:232	ESA products	221:232	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	2	40	theme	products	225:232	arg1	available					238:246	available	238:246	available	238:246	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	2	40	theme	products	225:232	arg1	variety					210:216	A variety	208:216	A variety of ESA products	208:232	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	3	41	theme	biological	442:451	arg1	activity					453:460	in vivo biological activity	434:460	in vivo biological activity	434:460	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	2	42	theme	ESA	221:223	arg1	products					225:232	ESA products	221:232	ESA products	221:232	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	1	43	theme	therapeutic	110:120	arg1	glycoproteins					122:134	therapeutic glycoproteins	110:134	therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	110:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	4	44	theme	analysis	532:539	arg1	combination					480:490	a combination	478:490	a combination of chromatography and mass spectrometry analysis	478:539	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	3	45	dep	in	434:435	arg1	vivo					437:440	vivo	437:440	vivo	437:440	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	7	46	theme	continued	1307:1315	arg1	monitoring					1317:1326	continued monitoring	1307:1326	continued monitoring of ESA product glycosylation	1307:1355	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	1	47	theme	glycoproteins	122:134	arg1	agents					81:86	Erythropoiesis stimulating agents	54:86	Erythropoiesis stimulating agents (ESAs)	54:93	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	47	theme	glycoproteins	122:134	arg1	group					101:105	a group	99:105	a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	99:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	47	theme	glycoproteins	122:134	arg1	glycoproteins					122:134	therapeutic glycoproteins	110:134	therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	110:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	4	48	theme	spectrometry	519:530	arg1	analysis					532:539	chromatography and mass spectrometry analysis	495:539	chromatography and mass spectrometry analysis	495:539	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	49	theme	alfa	737:740	arg1	biosimilar					742:751	epoetin alfa biosimilar	729:751	epoetin alfa biosimilar	729:751	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	49	theme	alfa	737:740	arg1	Silapo					721:726	Silapo	721:726	Silapo (epoetin alfa biosimilar)	721:752	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	2	50	theme	European	255:262	arg1	Union					264:268	the European Union	251:268	the European Union	251:268	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	6	51	theme	potencies	1262:1270	arg1	divergence					1236:1245	divergence	1236:1245	divergence of therapeutic potencies	1236:1270	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	2	52	from	Union	264:268	arg1	products					225:232	ESA products	221:232	ESA products	221:232	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	2	52	from	Union	264:268	arg1	available					238:246	available	238:246	available	238:246	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	2	52	from	Union	264:268	arg1	variety					210:216	A variety	208:216	A variety of ESA products	208:232	A variety of ESA products are available in the European Union, including innovator, biosimilar and second-generation medicines.					
31838284	7	53	theme	therapeutic	1433:1443	arg1	equivalence					1445:1455	therapeutic equivalence	1433:1455	therapeutic equivalence	1433:1455	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	6	54	gly	sulphated/penta-sialylated	1164:1189	arg1	N-glycans					1191:1199	sulphated/penta-sialylated N-glycans	1164:1199	sulphated/penta-sialylated N-glycans	1164:1199	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	4	55	dep	profiles	601:608	arg1	biosimilar					742:751	epoetin alfa biosimilar	729:751	epoetin alfa biosimilar	729:751	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	alfa					780:783	darbepoetin alfa	768:783	darbepoetin alfa	768:783	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	Binocrit®					684:692	Binocrit®	684:692	Binocrit® (epoetin alfa biosimilar)	684:718	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	Eprex®					632:637	Eprex®	632:637	Eprex® (epoetin alfa)	632:652	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	Silapo					721:726	Silapo	721:726	Silapo (epoetin alfa biosimilar)	721:752	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	NeoRecormon®					655:666	NeoRecormon®	655:666	NeoRecormon® (epoetin beta)	655:681	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	Aranesp®					758:765	Aranesp®	758:765	Aranesp® (darbepoetin alfa)	758:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	profiles					601:608	the glycosylation profiles	583:608	the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa)	583:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	biosimilar					708:717	epoetin alfa biosimilar	695:717	epoetin alfa biosimilar	695:717	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	alfa					648:651	epoetin alfa	640:651	epoetin alfa	640:651	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	55	dep	profiles	601:608	arg1	beta					677:680	epoetin beta	669:680	epoetin beta	669:680	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	56	theme	epoetin	729:735	arg1	biosimilar					742:751	epoetin alfa biosimilar	729:751	epoetin alfa biosimilar	729:751	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	56	theme	epoetin	729:735	arg1	Silapo					721:726	Silapo	721:726	Silapo (epoetin alfa biosimilar)	721:752	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	5	57	theme	N-glycan	900:907	arg1	identification					909:922	N-glycan identification	900:922	N-glycan identification	900:922	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	4	58	theme	glycosylation	587:599	arg1	Binocrit®					684:692	Binocrit®	684:692	Binocrit® (epoetin alfa biosimilar)	684:718	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	58	theme	glycosylation	587:599	arg1	Eprex®					632:637	Eprex®	632:637	Eprex® (epoetin alfa)	632:652	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	58	theme	glycosylation	587:599	arg1	Silapo					721:726	Silapo	721:726	Silapo (epoetin alfa biosimilar)	721:752	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	58	theme	glycosylation	587:599	arg1	NeoRecormon®					655:666	NeoRecormon®	655:666	NeoRecormon® (epoetin beta)	655:681	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	58	theme	glycosylation	587:599	arg1	Aranesp®					758:765	Aranesp®	758:765	Aranesp® (darbepoetin alfa)	758:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	4	58	theme	glycosylation	587:599	arg1	profiles					601:608	the glycosylation profiles	583:608	the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa)	583:784	In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa).					
31838284	7	59	theme	ESA	1331:1333	arg1	glycosylation					1343:1355	ESA product glycosylation	1331:1355	ESA product glycosylation	1331:1355	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	1	60	theme	Erythropoiesis	54:67	arg1	ESAs					89:92	ESAs	89:92	ESAs	89:92	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	60	theme	Erythropoiesis	54:67	arg1	agents					81:86	Erythropoiesis stimulating agents	54:86	Erythropoiesis stimulating agents (ESAs)	54:93	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	60	theme	Erythropoiesis	54:67	arg1	group					101:105	a group	99:105	a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	99:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	60	theme	Erythropoiesis	54:67	arg1	glycoproteins					122:134	therapeutic glycoproteins	110:134	therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	110:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	7	61	theme	product	1335:1341	arg1	glycosylation					1343:1355	ESA product glycosylation	1331:1355	ESA product glycosylation	1331:1355	The study highlights the need for continued monitoring of ESA product glycosylation, ideally allied to pharmacological data, in order to ensure consistency and therapeutic equivalence between products and enhance our understanding of ESA structure-activity-relationships.					
31838284	5	62	theme	anion-exchange/hydrophilic	827:852	arg1	chromatography					866:879	mixed-mode anion-exchange/hydrophilic interaction chromatography	816:879	mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation	816:983	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	5	62	theme	anion-exchange/hydrophilic	827:852	arg1	AEX/HILIC-MS					882:893	AEX/HILIC-MS	882:893	AEX/HILIC-MS	882:893	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	3	63	theme	in	434:435	arg1	activity					453:460	in vivo biological activity	434:460	in vivo biological activity	434:460	Glycosylation is a critical quality attribute of ESA products, as it has a crucial influence upon in vivo biological activity.					
31838284	6	64	contain	have	1208:1211	arg2	potential					1217:1225	the potential to cause divergence of therapeutic potencies	1213:1270	the potential to cause divergence of therapeutic potencies	1213:1270	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	64	contain	have	1208:1211	arg1	antennae					1151:1158	N-acetyllactosamine extended antennae	1122:1158	N-acetyllactosamine extended antennae	1122:1158	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	64	contain	have	1208:1211	arg1	N-glycans					1191:1199	sulphated/penta-sialylated N-glycans	1164:1199	sulphated/penta-sialylated N-glycans	1164:1199	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	64	contain	have	1208:1211	arg1	acid					1090:1093	sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans	1083:1199	acid	1090:1093	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	6	64	contain	have	1208:1211	arg1	O-acetylation					1107:1119	O-acetylation	1107:1119	O-acetylation	1107:1119	The products exhibit notable differences in N- and O-glycosylation, including attributes such as sialic acid occupation, O-acetylation, N-acetyllactosamine extended antennae and sulphated/penta-sialylated N-glycans, which have the potential to cause divergence of therapeutic potencies.					
31838284	1	65	theme	stimulating	69:79	arg1	ESAs					89:92	ESAs	89:92	ESAs	89:92	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	65	theme	stimulating	69:79	arg1	agents					81:86	Erythropoiesis stimulating agents	54:86	Erythropoiesis stimulating agents (ESAs)	54:93	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	65	theme	stimulating	69:79	arg1	group					101:105	a group	99:105	a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	99:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	1	65	theme	stimulating	69:79	arg1	glycoproteins					122:134	therapeutic glycoproteins	110:134	therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy	110:205	Erythropoiesis stimulating agents (ESAs) are a group of therapeutic glycoproteins used to treat anaemia caused by chronic kidney disease or chemotherapy.					
31838284	5	66	theme	interaction	854:864	arg1	chromatography					866:879	mixed-mode anion-exchange/hydrophilic interaction chromatography	816:879	mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation	816:983	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
31838284	5	66	theme	interaction	854:864	arg1	AEX/HILIC-MS					882:893	AEX/HILIC-MS	882:893	AEX/HILIC-MS	882:893	The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation.					
29648454	4	0	theme	glycan	493:498	arg1	structures					500:509	N-linked glycan structures	484:509	N-linked glycan structures	484:509	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	8	1	theme	pH-	980:982	arg1	ranges					1005:1010	optimal pH- and thermo-stability ranges	972:1010	optimal pH- and thermo-stability ranges	972:1010	The mutation did not change the substrate specificity and optimal pH- and thermo-stability ranges, but it increased the catalytic activity 2-3-fold.					
29648454	5	2	theme	Such	567:570	arg1	glycosylation					583:595	Such non-native glycosylation	567:595	Such non-native glycosylation	567:595	Such non-native glycosylation can have unwanted consequences.					
29648454	9	3	with	comparison	1066:1075	arg1	WPTP					1092:1095	wild-type WPTP	1082:1095	wild-type WPTP	1082:1095	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	3	4	theme	Pichia	342:347	arg1	system					389:394	an expression system	375:394	an expression system for WPTP and other peroxidases	375:425	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	3	4	theme	Pichia	342:347	arg1	pastoris					349:356	Pichia pastoris	342:356	Pichia pastoris	342:356	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	0	5	from	Effect	0:5	arg1	Stability					98:106	Stability	98:106	Stability	98:106	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	0	5	from	Effect	0:5	arg1	Activity					85:92	Activity	85:92	Activity	85:92	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	5	6	theme	non-native	572:581	arg1	glycosylation					583:595	Such non-native glycosylation	567:595	Such non-native glycosylation	567:595	Such non-native glycosylation can have unwanted consequences.					
29648454	4	7	from	site	561:564	arg1	level					530:534	the level	526:534	the level of glycosylation at each site	526:564	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	8	8	theme	thermo-stability	988:1003	arg1	ranges					1005:1010	optimal pH- and thermo-stability ranges	972:1010	optimal pH- and thermo-stability ranges	972:1010	The mutation did not change the substrate specificity and optimal pH- and thermo-stability ranges, but it increased the catalytic activity 2-3-fold.					
29648454	5	9	contain	have	601:604	arg1	glycosylation					583:595	Such non-native glycosylation	567:595	Such non-native glycosylation	567:595	Such non-native glycosylation can have unwanted consequences.					
29648454	5	9	contain	have	601:604	arg2	consequences					615:626	unwanted consequences	606:626	unwanted consequences	606:626	Such non-native glycosylation can have unwanted consequences.					
29648454	6	10	gly	under-glycosylated	657:674	arg1	%					697:697	1.5%	694:697	1.5%	694:697	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	10	gly	under-glycosylated	657:674	arg1	wild-type					683:691	wild-type	683:691	wild-type	683:691	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	10	gly	under-glycosylated	657:674	arg1	N256					648:651	Glycosylation site N256	629:651	Glycosylation site N256	629:651	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	10	gly	under-glycosylated	657:674	arg2	N256					648:651	Glycosylation site N256	629:651	Glycosylation site N256	629:651	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	8	11	theme	substrate	946:954	arg1	specificity					956:966	the substrate specificity	942:966	the substrate specificity	942:966	The mutation did not change the substrate specificity and optimal pH- and thermo-stability ranges, but it increased the catalytic activity 2-3-fold.					
29648454	9	12	theme	stable	1136:1141	arg1	pH					1143:1144	the most stable pH	1127:1144	the most stable pH from 7 to 9	1127:1156	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	3	13	theme	other	409:413	arg1	peroxidases					415:425	other peroxidases	409:425	other peroxidases	409:425	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	3	14	theme	expression	378:387	arg1	system					389:394	an expression system	375:394	an expression system for WPTP and other peroxidases	375:425	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	3	14	theme	expression	378:387	arg1	pastoris					349:356	Pichia pastoris	342:356	Pichia pastoris	342:356	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	4	15	theme	different	465:473	arg1	types					475:479	different types	465:479	different types of N-linked glycan structures	465:509	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	5	16	theme	unwanted	606:613	arg1	consequences					615:626	unwanted consequences	606:626	unwanted consequences	606:626	Such non-native glycosylation can have unwanted consequences.					
29648454	7	17	theme	chromatography-tandem	873:893	arg1	spectrometry					900:911	liquid chromatography-tandem mass spectrometry	866:911	liquid chromatography-tandem mass spectrometry	866:911	Glycosylation increased four-fold, as measured by liquid chromatography-tandem mass spectrometry.					
29648454	9	18	from	9	1156:1156	arg1	pH					1143:1144	the most stable pH	1127:1144	the most stable pH from 7 to 9	1127:1156	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	9	18	from	9	1156:1156	arg1	shift					1118:1122	a shift	1116:1122	a shift of the most stable pH from 7 to 9	1116:1156	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	7	19	theme	mass	895:898	arg1	spectrometry					900:911	liquid chromatography-tandem mass spectrometry	866:911	liquid chromatography-tandem mass spectrometry	866:911	Glycosylation increased four-fold, as measured by liquid chromatography-tandem mass spectrometry.					
29648454	6	20	gly	glycosylation	775:787	arg1	site					797:800	this site	792:800	this site (WPTP E254G)	792:813	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	20	gly	glycosylation	775:787	arg1	E254G					808:812	WPTP E254G	803:812	WPTP E254G	803:812	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	8	21	theme	optimal	972:978	arg1	ranges					1005:1010	optimal pH- and thermo-stability ranges	972:1010	optimal pH- and thermo-stability ranges	972:1010	The mutation did not change the substrate specificity and optimal pH- and thermo-stability ranges, but it increased the catalytic activity 2-3-fold.					
29648454	6	22	from	under-glycosylated	657:674	arg1	wild-type					683:691	wild-type	683:691	wild-type	683:691	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	22	from	under-glycosylated	657:674	arg1	%					697:697	1.5%	694:697	1.5%	694:697	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	23	theme	WPTP	803:806	arg1	site					797:800	this site	792:800	this site (WPTP E254G)	792:813	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	23	theme	WPTP	803:806	arg1	E254G					808:812	WPTP E254G	803:812	WPTP E254G	803:812	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	0	24	theme	Glycosylation	19:31	arg1	Effect					0:5	Effect	0:5	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.	0:107	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	4	25	link	N-linked	484:491	arg1	structures					500:509	N-linked glycan structures	484:509	N-linked glycan structures	484:509	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	8	26	theme	catalytic	1034:1042	arg1	activity					1044:1051	the catalytic activity	1030:1051	the catalytic activity	1030:1051	The mutation did not change the substrate specificity and optimal pH- and thermo-stability ranges, but it increased the catalytic activity 2-3-fold.					
29648454	7	27	theme	liquid	866:871	arg1	spectrometry					900:911	liquid chromatography-tandem mass spectrometry	866:911	liquid chromatography-tandem mass spectrometry	866:911	Glycosylation increased four-fold, as measured by liquid chromatography-tandem mass spectrometry.					
29648454	6	28	from	wild-type	683:691	arg1	under-glycosylated					657:674	under-glycosylated	657:674	under-glycosylated	657:674	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	0	29	theme	N-Linked	10:17	arg1	Glycosylation					19:31	N-Linked Glycosylation	10:31	N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase	10:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	4	30	theme	glycosylation	539:551	arg1	level					530:534	the level	526:534	the level of glycosylation at each site	526:564	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	2	31	theme	tree	188:191	arg1	fortunei					206:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	0	32	theme	Recombinant	36:46	arg1	Peroxidase					67:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	2	33	theme	palm	183:186	arg1	fortunei					206:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	34	theme	stable	240:245	arg1	WPTP					272:275	WPTP	272:275	WPTP	272:275	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	34	theme	stable	240:245	arg1	peroxidases					259:269	the most stable and fastest peroxidases	231:269	the most stable and fastest peroxidases (WPTP) characterized to date	231:298	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	3	35	used	used	367:370	arg2	pastoris					349:356	Pichia pastoris	342:356	Pichia pastoris	342:356	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	3	35	used	used	367:370	arg2	system					389:394	an expression system	375:394	an expression system for WPTP and other peroxidases	375:425	Pichia pastoris has been used as an expression system for WPTP and other peroxidases.					
29648454	2	36	theme	windmill	174:181	arg1	fortunei					206:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	9	37	theme	wild-type	1082:1090	arg1	WPTP					1092:1095	wild-type WPTP	1082:1095	wild-type WPTP	1082:1095	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	0	38	theme	Palm	57:60	arg1	Peroxidase					67:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	4	39	theme	structures	500:509	arg1	types					475:479	different types	465:479	different types of N-linked glycan structures	465:509	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	9	40	from	shift	1118:1122	arg1	9					1156:1156	9	1156:1156	9	1156:1156	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	1	41	theme	Plant	109:113	arg1	peroxidases					125:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	1	41	theme	Plant	109:113	arg1	enzymes					161:167	valuable commercial enzymes	141:167	valuable commercial enzymes	141:167	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	0	42	theme	Windmill	48:55	arg1	Peroxidase					67:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	0	43	gly	Glycosylation	19:31	arg1	Peroxidase					67:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	1	44	theme	secretory	115:123	arg1	peroxidases					125:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	1	44	theme	secretory	115:123	arg1	enzymes					161:167	valuable commercial enzymes	141:167	valuable commercial enzymes	141:167	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	9	45	theme	pH	1143:1144	arg1	shift					1118:1122	a shift	1116:1122	a shift of the most stable pH from 7 to 9	1116:1156	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	6	46	dep	under-glycosylated	657:674	arg1	%					733:733	55%	731:733	55%	731:733	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	2	47	theme	economical	313:322	arg1	source					324:329	an economical source	310:329	an economical source	310:329	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	9	48	theme	WPTP	1098:1101	arg1	E254G					1103:1107	WPTP E254G	1098:1107	WPTP E254G	1098:1107	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	4	49	theme	N-linked	484:491	arg1	structures					500:509	N-linked glycan structures	484:509	N-linked glycan structures	484:509	However, yeast and plants synthesize different types of N-linked glycan structures and may differ the level of glycosylation at each site.					
29648454	2	50	theme	peroxidases	259:269	arg1	WPTP					272:275	WPTP	272:275	WPTP	272:275	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	50	theme	peroxidases	259:269	arg1	peroxidases					259:269	the most stable and fastest peroxidases	231:269	the most stable and fastest peroxidases (WPTP) characterized to date	231:298	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	50	theme	peroxidases	259:269	arg1	one					224:226	one	224:226	one	224:226	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	6	51	theme	native	716:721	arg1	enzyme					723:728	the native enzyme	712:728	the native enzyme	712:728	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	6	52	theme	site	643:646	arg1	N256					648:651	Glycosylation site N256	629:651	Glycosylation site N256	629:651	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	0	53	theme	Peroxidase	67:76	arg1	Glycosylation					19:31	N-Linked Glycosylation	10:31	N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase	10:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	2	54	theme	Trachycarpus	193:204	arg1	fortunei					206:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei	170:213	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	55	theme	fastest	251:257	arg1	WPTP					272:275	WPTP	272:275	WPTP	272:275	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	2	55	theme	fastest	251:257	arg1	peroxidases					259:269	the most stable and fastest peroxidases	231:269	the most stable and fastest peroxidases (WPTP) characterized to date	231:298	The windmill palm tree Trachycarpus fortunei produces one of the most stable and fastest peroxidases (WPTP) characterized to date; however, an economical source is needed.					
29648454	1	56	theme	valuable	141:148	arg1	peroxidases					125:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	1	56	theme	valuable	141:148	arg1	enzymes					161:167	valuable commercial enzymes	141:167	valuable commercial enzymes	141:167	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	6	57	theme	Glycosylation	629:641	arg1	N256					648:651	Glycosylation site N256	629:651	Glycosylation site N256	629:651	Glycosylation site N256 was under-glycosylated in the wild-type (1.5%) compared to the native enzyme (55%); therefore, we mutated WPTP to promote glycosylation at this site (WPTP E254G).					
29648454	9	58	dep	9	1156:1156	arg1	to					1153:1154	to	1153:1154	to	1153:1154	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	0	59	theme	Tree	62:65	arg1	Peroxidase					67:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Recombinant Windmill Palm Tree Peroxidase	36:76	Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability.					
29648454	9	60	theme	alkaline	1201:1208	arg1	conditions					1210:1219	alkaline conditions	1201:1219	alkaline conditions	1201:1219	In comparison with wild-type WPTP, WPTP E254G showed a shift of the most stable pH from 7 to 9, making it suitable for applications under alkaline conditions.					
29648454	1	61	theme	commercial	150:159	arg1	peroxidases					125:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases	109:135	Plant secretory peroxidases are valuable commercial enzymes.					
29648454	1	61	theme	commercial	150:159	arg1	enzymes					161:167	valuable commercial enzymes	141:167	valuable commercial enzymes	141:167	Plant secretory peroxidases are valuable commercial enzymes.					
31598098	0	0	theme	PNGase	80:85	arg1	F					87:87	PNGase F	80:87	PNGase F	80:87	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	3	1	link	N-linked	402:409	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans chemically using sodium hypochlorite (NaOCl)	402:462	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	1	2	theme	chemical	140:147	arg1	methods					126:132	several methods	118:132	several methods	118:132	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	5	3	theme	NaOCl	883:887	arg1	protocol					889:896	the NaOCl protocol	879:896	the NaOCl protocol	879:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	4	link	N-linked	970:977	arg1	glycans					979:985	N-linked glycans	970:985	N-linked glycans	970:985	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	0	5	theme	Release	89:95	arg1	Protocols					97:105	Release Protocols	89:105	Release Protocols	89:105	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	4	6	theme	liquid	726:731	arg1	spectrometry					780:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	1	7	link	N-linked	175:182	arg1	glycans					184:190	N-linked glycans	175:190	N-linked glycans	175:190	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	3	8	theme	quicker	345:351	arg1	alternative					353:363	A less expensive and quicker alternative	324:363	A less expensive and quicker alternative	324:363	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	4	9	theme	small-scale	690:700	arg1	analysis					702:709	small-scale analysis	690:709	small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry	690:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	4	9	theme	small-scale	690:700	arg1	µg					716:717	300 µg	712:717	300 µg	712:717	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	4	10	theme	chromatography-single	733:753	arg1	spectrometry					780:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	4	11	theme	release	669:675	arg1	protocol					677:684	the PNGaseF release protocol	657:684	the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry	657:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	4	12	theme	PNGaseF	661:667	arg1	protocol					677:684	the PNGaseF release protocol	657:684	the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry	657:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	0	13	link	N-linked	0:7	arg1	Efficiency					24:33	N-linked Glycan Release Efficiency	0:33	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	2	14	theme	methods	266:272	arg1	One					225:227	One	225:227	One	225:227	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	14	theme	methods	266:272	arg1	methods					266:272	the most common enzymatic release methods	232:272	the most common enzymatic release methods	232:272	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	14	theme	methods	266:272	arg1	use					281:283	the use	277:283	the use of peptide:N-glycosidase F (PNGase F)	277:321	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	5	15	theme	released	848:855	arg1	glycans					866:872	released N-linked glycans	848:872	released N-linked glycans from the NaOCl protocol	848:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	1	16	theme	enzymatic	153:161	arg1	methods					126:132	several methods	118:132	several methods	118:132	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	5	17	link	N-linked	857:864	arg1	glycans					866:872	released N-linked glycans	848:872	released N-linked glycans from the NaOCl protocol	848:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	2	18	theme	release	258:264	arg1	methods					266:272	the most common enzymatic release methods	232:272	the most common enzymatic release methods	232:272	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	0	19	theme	Glycan	9:14	arg1	Efficiency					24:33	N-linked Glycan Release Efficiency	0:33	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	3	20	theme	intact	520:525	arg1	glycan					527:532	the intact glycan	516:532	the intact glycan	516:532	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	4	21	theme	NaOCl	620:624	arg1	protocol					634:641	the NaOCl release protocol	616:641	the NaOCl release protocol	616:641	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	0	22	theme	N-linked	0:7	arg1	Efficiency					24:33	N-linked Glycan Release Efficiency	0:33	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	5	23	from	protocol	889:896	arg1	glycans					866:872	released N-linked glycans	848:872	released N-linked glycans from the NaOCl protocol	848:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	23	from	protocol	889:896	arg1	similar					901:907	similar	901:907	similar	901:907	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	23	from	protocol	889:896	arg1	composition					833:843	the relative glycan composition	813:843	the relative glycan composition of released N-linked glycans from the NaOCl protocol	813:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	2	24	theme	peptide	288:294	arg1	One					225:227	One	225:227	One	225:227	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	24	theme	peptide	288:294	arg1	methods					266:272	the most common enzymatic release methods	232:272	the most common enzymatic release methods	232:272	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	24	theme	peptide	288:294	arg1	use					281:283	the use	277:283	the use of peptide:N-glycosidase F (PNGase F)	277:321	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	5	25	theme	N-linked	857:864	arg1	glycans					866:872	released N-linked glycans	848:872	released N-linked glycans from the NaOCl protocol	848:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	0	26	dep	NaOCl	70:74	arg1	Protocols					97:105	Release Protocols	89:105	Release Protocols	89:105	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	5	27	theme	glycans	979:985	arg1	recovery					958:965	the absolute recovery	945:965	the absolute recovery of N-linked glycans	945:985	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	27	theme	glycans	979:985	arg1	lower					1004:1008	lower	1004:1008	lower	1004:1008	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	1	28	theme	N-linked	175:182	arg1	glycans					184:190	N-linked glycans	175:190	N-linked glycans	175:190	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	0	29	theme	Release	16:22	arg1	Efficiency					24:33	N-linked Glycan Release Efficiency	0:33	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	5	30	theme	typical	914:920	arg1	protocol					931:938	a typical PNGase F protocol	912:938	a typical PNGase F protocol	912:938	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	31	theme	F	929:929	arg1	protocol					931:938	a typical PNGase F protocol	912:938	a typical PNGase F protocol	912:938	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	32	theme	glycans	866:872	arg1	similar					901:907	similar	901:907	similar	901:907	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	32	theme	glycans	866:872	arg1	composition					833:843	the relative glycan composition	813:843	the relative glycan composition of released N-linked glycans from the NaOCl protocol	813:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	33	theme	N-linked	970:977	arg1	glycans					979:985	N-linked glycans	970:985	N-linked glycans	970:985	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	34	theme	relative	817:824	arg1	similar					901:907	similar	901:907	similar	901:907	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	34	theme	relative	817:824	arg1	composition					833:843	the relative glycan composition	813:843	the relative glycan composition of released N-linked glycans from the NaOCl protocol	813:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	3	35	theme	free	541:544	arg1	terminus					555:562	a free reducing terminus	539:562	a free reducing terminus	539:562	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	3	36	theme	N-linked	402:409	arg1	glycans					411:417	N-linked glycans	402:417	N-linked glycans chemically using sodium hypochlorite (NaOCl)	402:462	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	3	37	theme	reducing	546:553	arg1	terminus					555:562	a free reducing terminus	539:562	a free reducing terminus	539:562	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	5	38	theme	glycan	826:831	arg1	similar					901:907	similar	901:907	similar	901:907	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	38	theme	glycan	826:831	arg1	composition					833:843	the relative glycan composition	813:843	the relative glycan composition of released N-linked glycans from the NaOCl protocol	813:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	1	39	theme	structural	196:205	arg1	characterization					207:222	structural characterization	196:222	structural characterization	196:222	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	2	40	theme	PNGase	313:318	arg1	F					310:310	N-glycosidase F	296:310	the use of peptide:N-glycosidase F (PNGase F)	277:321	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	40	theme	PNGase	313:318	arg1	F					320:320	PNGase F	313:320	PNGase F	313:320	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	0	41	theme	Quantitative	38:49	arg1	Comparison					51:60	A Quantitative Comparison	36:60	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	2	42	dep	use	281:283	arg1	F					310:310	N-glycosidase F	296:310	the use of peptide:N-glycosidase F (PNGase F)	277:321	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	42	dep	use	281:283	arg1	F					320:320	PNGase F	313:320	PNGase F	313:320	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	4	43	theme	reaction	755:762	arg1	spectrometry					780:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	0	44	dep	Efficiency	24:33	arg1	Comparison					51:60	A Quantitative Comparison	36:60	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.	0:106	N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols.					
31598098	2	45	theme	enzymatic	248:256	arg1	methods					266:272	the most common enzymatic release methods	232:272	the most common enzymatic release methods	232:272	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	1	46	theme	several	118:124	arg1	methods					126:132	several methods	118:132	several methods	118:132	There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization.					
31598098	5	47	theme	PNGase	922:927	arg1	protocol					931:938	a typical PNGase F protocol	912:938	a typical PNGase F protocol	912:938	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	3	48	theme	peptide-glycan	486:499	arg1	bond					501:504	the peptide-glycan bond	482:504	the peptide-glycan bond	482:504	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	5	49	theme	chemical	1019:1026	arg1	procedure					1028:1036	the chemical procedure	1015:1036	the chemical procedure	1015:1036	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	2	50	theme	common	241:246	arg1	methods					266:272	the most common enzymatic release methods	232:272	the most common enzymatic release methods	232:272	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	51	theme	N-glycosidase	296:308	arg1	F					310:310	N-glycosidase F	296:310	the use of peptide:N-glycosidase F (PNGase F)	277:321	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	2	51	theme	N-glycosidase	296:308	arg1	F					320:320	PNGase F	313:320	PNGase F	313:320	One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F).					
31598098	3	52	theme	sodium	436:441	arg1	NaOCl					457:461	NaOCl	457:461	NaOCl	457:461	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	3	52	theme	sodium	436:441	arg1	hypochlorite					443:454	sodium hypochlorite	436:454	sodium hypochlorite (NaOCl)	436:462	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	3	53	theme	glycans	411:417	arg1	release					391:397	the release	387:397	the release	387:397	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	4	54	theme	monitoring-mass	764:778	arg1	spectrometry					780:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	liquid chromatography-single reaction monitoring-mass spectrometry	726:791	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	4	55	theme	protocol	634:641	arg1	efficiency					602:611	the efficiency	598:611	the efficiency of the NaOCl release protocol	598:641	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
31598098	5	56	theme	absolute	949:956	arg1	recovery					958:965	the absolute recovery	945:965	the absolute recovery of N-linked glycans	945:985	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	5	56	theme	absolute	949:956	arg1	lower					1004:1008	lower	1004:1008	lower	1004:1008	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	3	57	theme	expensive	331:339	arg1	alternative					353:363	A less expensive and quicker alternative	324:363	A less expensive and quicker alternative	324:363	A less expensive and quicker alternative has been reported for the release of N-linked glycans chemically using sodium hypochlorite (NaOCl), which hydrolyzes the peptide-glycan bond, yielding the intact glycan with a free reducing terminus.					
31598098	5	58	from	composition	833:843	arg1	protocol					889:896	the NaOCl protocol	879:896	the NaOCl protocol	879:896	We determined that the relative glycan composition of released N-linked glycans from the NaOCl protocol is similar to a typical PNGase F protocol, but the absolute recovery of N-linked glycans is significantly lower with the chemical procedure.					
31598098	4	59	theme	release	626:632	arg1	protocol					634:641	the NaOCl release protocol	616:641	the NaOCl release protocol	616:641	Here, we quantitatively analyzed the efficiency of the NaOCl release protocol compared with the PNGaseF release protocol for small-scale analysis (300 µg) using liquid chromatography-single reaction monitoring-mass spectrometry.					
30276752	4	0	theme	additional	748:757	arg1	enrichment					768:777	An additional N-glycan enrichment	745:777	An additional N-glycan enrichment	745:777	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	4	1	theme	single	828:833	arg1	structures					835:844	single structures	828:844	single structures	828:844	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	1	2	theme	possible	178:185	arg1	heterogeneity					187:199	possible heterogeneity	178:199	possible heterogeneity due to different structures	178:227	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	3	theme	posttranslational	71:87	arg1	N-Glycans					57:65	N-Glycans	57:65	N-Glycans	57:65	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	3	theme	posttranslational	71:87	arg1	modifications					89:101	posttranslational modifications	71:101	posttranslational modifications of proteins attached to the amide side chains of asparagine residues	71:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	2	4	theme	invertebrate	314:325	arg1	N-glycosylation					327:341	invertebrate N-glycosylation	314:341	invertebrate N-glycosylation	314:341	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	3	5	gly	glycoproteins	555:567	arg1	glycoproteins					555:567	specific glycoproteins	546:567	specific glycoproteins	546:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	6	gly	glycosylation	256:268	arg2	site					270:273	the same glycosylation site	247:273	the same glycosylation site	247:273	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	2	7	theme	mammalian	295:303	arg1	systems					305:311	the mammalian systems	291:311	the mammalian systems	291:311	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	3	8	dep	peptide	623:629	arg1	spectrometry					636:647	mass spectrometry	631:647	mass spectrometry	631:647	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	3	8	dep	peptide	623:629	arg1	MS/MS					682:686	RP-HPLC MALDI-TOF MS/MS	664:686	RP-HPLC MALDI-TOF MS/MS	664:686	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	4	9	from	proteins	910:917	arg1	separation					872:881	isomeric separation	863:881	isomeric separation of N-glycans from specific proteins	863:917	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	4	10	theme	specific	901:908	arg1	proteins					910:917	specific proteins	901:917	specific proteins	901:917	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	1	11	theme	proteins	106:113	arg1	N-Glycans					57:65	N-Glycans	57:65	N-Glycans	57:65	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	11	theme	proteins	106:113	arg1	modifications					89:101	posttranslational modifications	71:101	posttranslational modifications of proteins attached to the amide side chains of asparagine residues	71:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	2	12	theme	high	417:420	arg1	degree					422:427	a high degree	415:427	a high degree of structural and isomeric variation between different species	415:490	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	2	13	dep	systems	305:311	arg1	contrast					279:286	contrast	279:286	contrast	279:286	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	0	14	theme	Protein-Specific	0:15	arg1	Analysis					17:24	Protein-Specific Analysis	0:24	Protein-Specific Analysis of Invertebrate Glycoproteins	0:54	Protein-Specific Analysis of Invertebrate Glycoproteins.					
30276752	2	15	attach	presents	343:350	arg1	analysis					367:374	analysis	367:374	analysis	367:374	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	2	15	attach	presents	343:350	arg2	N-glycosylation					327:341	invertebrate N-glycosylation	314:341	invertebrate N-glycosylation	314:341	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	3	16	theme	tryptic	615:621	arg1	peptide					623:629	tryptic peptide	615:629	tryptic peptide	615:629	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	17	theme	different	208:216	arg1	structures					218:227	different structures	208:227	different structures	208:227	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	0	18	theme	Invertebrate	29:40	arg1	Glycoproteins					42:54	Invertebrate Glycoproteins	29:54	Invertebrate Glycoproteins	29:54	Protein-Specific Analysis of Invertebrate Glycoproteins.					
30276752	3	19	from	N-glycans	533:541	arg1	glycoproteins					555:567	specific glycoproteins	546:567	specific glycoproteins	546:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	2	20	theme	isomeric	447:454	arg1	variation					456:464	structural and isomeric variation	432:464	structural and isomeric variation between different species	432:490	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	2	21	theme	variation	456:464	arg1	degree					422:427	a high degree	415:427	a high degree of structural and isomeric variation between different species	415:490	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	2	21	theme	variation	456:464	arg1	epitopes					402:409	unfamiliar epitopes	391:409	unfamiliar epitopes	391:409	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	2	22	theme	different	474:482	arg1	species					484:490	different species	474:490	different species	474:490	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	1	23	theme	amide	131:135	arg1	chains					142:147	the amide side chains	127:147	the amide side chains of asparagine residues	127:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	3	24	theme	analytical	502:511	arg1	approach					513:520	A simple analytical approach	493:520	A simple analytical approach to analyze N-glycans on specific glycoproteins	493:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	2	25	theme	structural	432:441	arg1	variation					456:464	structural and isomeric variation	432:464	structural and isomeric variation between different species	432:490	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30276752	0	26	theme	Glycoproteins	42:54	arg1	Analysis					17:24	Protein-Specific Analysis	0:24	Protein-Specific Analysis of Invertebrate Glycoproteins	0:54	Protein-Specific Analysis of Invertebrate Glycoproteins.					
30276752	3	27	theme	MALDI-TOF	672:680	arg1	MS/MS					682:686	RP-HPLC MALDI-TOF MS/MS	664:686	RP-HPLC MALDI-TOF MS/MS	664:686	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	28	attach	attached	115:122	arg2	proteins					106:113	proteins	106:113	proteins attached to the amide side chains of asparagine residues	106:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	28	attach	attached	115:122	arg1	chains					142:147	the amide side chains	127:147	the amide side chains of asparagine residues	127:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	4	29	theme	N-glycans	886:894	arg1	separation					872:881	isomeric separation	863:881	isomeric separation of N-glycans from specific proteins	863:917	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	3	30	theme	peptide	623:629	arg1	combination					600:610	a combination	598:610	a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes	598:742	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	31	theme	side	137:140	arg1	chains					142:147	the amide side chains	127:147	the amide side chains of asparagine residues	127:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	32	from	site	270:273	arg1	possible					235:242	possible	235:242	possible	235:242	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	3	33	dep	approach	513:520	arg1	analyze					525:531	analyze	525:531	to analyze N-glycans on specific glycoproteins	522:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	3	34	theme	mass	631:634	arg1	spectrometry					636:647	mass spectrometry	631:647	mass spectrometry	631:647	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	3	35	theme	specific	546:553	arg1	glycoproteins					555:567	specific glycoproteins	546:567	specific glycoproteins	546:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	4	36	theme	N-glycan	759:766	arg1	enrichment					768:777	An additional N-glycan enrichment	745:777	An additional N-glycan enrichment	745:777	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	3	37	theme	specific	726:733	arg1	epitopes					735:742	specific epitopes	726:742	specific epitopes	726:742	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	3	38	theme	"	662:662	arg1	combination					600:610	a combination	598:610	a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes	598:742	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	4	39	theme	labeling	783:790	arg1	step					792:795	labeling step	783:795	labeling step	783:795	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	4	40	theme	structures	835:844	arg1	analysis					816:823	the analysis	812:823	the analysis of single structures	812:844	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	3	41	theme	RP-HPLC	664:670	arg1	MS/MS					682:686	RP-HPLC MALDI-TOF MS/MS	664:686	RP-HPLC MALDI-TOF MS/MS	664:686	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	42	theme	asparagine	152:161	arg1	residues					163:170	asparagine residues	152:170	asparagine residues	152:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	43	theme	same	251:254	arg1	site					270:273	the same glycosylation site	247:273	the same glycosylation site	247:273	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	4	44	theme	isomeric	863:870	arg1	separation					872:881	isomeric separation	863:881	isomeric separation of N-glycans from specific proteins	863:917	An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.					
30276752	3	45	theme	simple	495:500	arg1	approach					513:520	A simple analytical approach	493:520	A simple analytical approach to analyze N-glycans on specific glycoproteins	493:567	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	1	46	theme	residues	163:170	arg1	chains					142:147	the amide side chains	127:147	the amide side chains of asparagine residues	127:170	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	1	47	theme	glycosylation	256:268	arg1	site					270:273	the same glycosylation site	247:273	the same glycosylation site	247:273	N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site.					
30276752	3	48	theme	off-line	654:661	arg1	"					662:662	"off-line"	653:662	"off-line"	653:662	A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes.					
30276752	2	49	theme	unfamiliar	391:400	arg1	epitopes					402:409	unfamiliar epitopes	391:409	unfamiliar epitopes	391:409	In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species.					
30463578	8	0	theme	same	1292:1295	arg1	change					1297:1302	the same change	1288:1302	the same change replicated in IgG glycome	1288:1328	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	4	1	theme	immunoglobulin	596:609	arg1	G					611:611	immunoglobulin G	596:611	immunoglobulin G (IgG) glycosylation profiles	596:640	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	4	1	theme	immunoglobulin	596:609	arg1	IgG					614:616	IgG	614:616	IgG	614:616	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	7	2	theme	trisialylated	1137:1149	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	2	theme	trisialylated	1137:1149	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	3	from	increase	1072:1079	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	3	from	increase	1072:1079	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	3	from	increase	1072:1079	arg1	branched					1044:1051	branched	1044:1051	branched	1044:1051	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	4	theme	tetragalactosylated	1116:1134	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	4	theme	tetragalactosylated	1116:1134	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	2	5	theme	spirometric	298:308	arg1	parameters					310:319	similar spirometric parameters	290:319	similar spirometric parameters	290:319	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	11	6	dep	CONCLUSIONS	1709:1719	arg1	showed					1732:1737	showed	1732:1737	showed that complexity of glycans associates with COPD, mirroring also the disease severity	1732:1822	CONCLUSIONS This study showed that complexity of glycans associates with COPD, mirroring also the disease severity.					
30463578	9	7	theme	complex	1454:1460	arg1	structures					1469:1478	more complex glycan structures	1449:1478	more complex glycan structures	1449:1478	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	4	8	theme	healthy	668:674	arg1	controls					676:683	healthy controls	668:683	healthy controls	668:683	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	2	9	with	subjects	276:283	arg1	parameters					310:319	similar spirometric parameters	290:319	similar spirometric parameters	290:319	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	7	10	theme	complex	1089:1095	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	10	theme	complex	1089:1095	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	13	11	theme	IgG	1992:1994	arg1	changes					2006:2012	plasma protein and IgG N-glycome changes	1973:2012	changes	2006:2012	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	9	12	theme	glycan	1462:1467	arg1	structures					1469:1478	more complex glycan structures	1449:1478	more complex glycan structures	1449:1478	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	7	13	gly	trisialylated	1137:1149	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	13	gly	trisialylated	1137:1149	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	9	14	theme	disease	1497:1503	arg1	progressed					1505:1514	the disease progressed	1493:1514	the disease progressed	1493:1514	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	7	15	from	decrease	1028:1035	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	15	from	decrease	1028:1035	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	15	from	decrease	1028:1035	arg1	branched					1044:1051	branched	1044:1051	branched	1044:1051	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	10	16	theme	major	1669:1673	arg1	factor					1680:1685	the major risk factor	1665:1685	the major risk factor for COPD development	1665:1706	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	10	16	theme	major	1669:1673	arg1	smoking					1647:1653	smoking	1647:1653	smoking	1647:1653	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	5	17	theme	IgG	728:730	arg1	N-glycome					732:740	IgG N-glycome	728:740	IgG N-glycome	728:740	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	0	18	theme	plasma	28:33	arg1	proteins					35:42	plasma proteins	28:42	plasma proteins	28:42	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	5	19	theme	matching	807:814	arg1	controls					816:823	95 matching controls	804:823	95 matching controls from Croatia	804:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	6	20	theme	medical	940:946	arg1	centre					948:953	another Croatian medical centre	923:953	another Croatian medical centre	923:953	Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre.					
30463578	8	21	theme	IgG	1318:1320	arg1	glycome					1322:1328	IgG glycome	1318:1328	IgG glycome	1318:1328	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	5	22	from	total	768:772	arg1	Croatia					830:836	Croatia	830:836	Croatia	830:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	1	23	theme	pulmonary	138:146	arg1	disease					148:154	BACKGROUND Chronic obstructive pulmonary disease	107:154	BACKGROUND Chronic obstructive pulmonary disease (COPD)	107:161	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	23	theme	pulmonary	138:146	arg1	condition					176:184	a complex condition	166:184	a complex condition	166:184	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	23	theme	pulmonary	138:146	arg1	COPD					157:160	COPD	157:160	COPD	157:160	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	7	24	dep	RESULTS	956:962	arg1	exhibited					1006:1014	exhibited	1006:1014	exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1006:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	5	25	theme	total	703:707	arg1	protein					716:722	total plasma protein	703:722	total plasma protein	703:722	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	7	26	theme	COPD	992:995	arg1	subjects					997:1004	COPD subjects	992:1004	COPD subjects	992:1004	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	27	theme	significant	1016:1026	arg1	decrease					1028:1035	significant decrease	1016:1035	significant decrease in low branched	1016:1051	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	13	28	theme	plasma	1973:1978	arg1	protein					1980:1986	plasma protein and IgG N-glycome changes	1973:2012	protein	1980:1986	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	5	29	theme	patients	781:788	arg1	total					768:772	the total	764:772	the total of 137 patients with COPD and 95 matching controls from Croatia	764:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	0	30	theme	obstructive	76:86	arg1	disease					98:104	chronic obstructive pulmonary disease	68:104	chronic obstructive pulmonary disease	68:104	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	7	31	theme	Plasma	964:969	arg1	N-glycome					979:987	Plasma protein N-glycome	964:987	Plasma protein N-glycome in COPD subjects	964:1004	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	11	32	theme	disease	1807:1813	arg1	severity					1815:1822	the disease severity	1803:1822	the disease severity	1803:1822	CONCLUSIONS This study showed that complexity of glycans associates with COPD, mirroring also the disease severity.					
30463578	13	33	theme	disease	2079:2085	arg1	progression					2087:2097	the disease progression	2075:2097	the disease progression	2075:2097	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	5	34	dep	METHODS	686:692	arg1	Both					694:697	Both	694:697	Both	694:697	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	4	35	theme	potential	486:494	arg1	value					496:500	the potential value	482:500	the potential value of N-glycans as COPD biomarkers	482:532	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	0	36	theme	N-glycosylation	0:14	arg1	patterns					16:23	N-glycosylation patterns	0:23	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.	0:105	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	12	37	from	changes	1835:1841	arg1	N-glycome					1846:1854	N-glycome	1846:1854	N-glycome	1846:1854	Moreover, changes in N-glycome associate with exacerbation frequency and are affected by smoking.					
30463578	5	38	theme	controls	816:823	arg1	total					768:772	the total	764:772	the total of 137 patients with COPD and 95 matching controls from Croatia	764:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	1	39	theme	Chronic	118:124	arg1	disease					148:154	BACKGROUND Chronic obstructive pulmonary disease	107:154	BACKGROUND Chronic obstructive pulmonary disease (COPD)	107:161	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	39	theme	Chronic	118:124	arg1	condition					176:184	a complex condition	166:184	a complex condition	166:184	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	39	theme	Chronic	118:124	arg1	COPD					157:160	COPD	157:160	COPD	157:160	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	4	40	theme	plasma	577:582	arg1	protein					584:590	plasma protein	577:590	plasma protein	577:590	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	4	41	with	controls	676:683	arg1	COPD					659:662	COPD	659:662	COPD	659:662	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	7	42	theme	antennary	1172:1180	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	42	theme	antennary	1172:1180	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	9	43	theme	different	1389:1397	arg1	stages					1409:1414	different COPD GOLD stages	1389:1414	different COPD GOLD stages	1389:1414	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	0	44	theme	proteins	35:42	arg1	patterns					16:23	N-glycosylation patterns	0:23	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.	0:105	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	7	45	gly	tetrasialylated	1152:1166	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	45	gly	tetrasialylated	1152:1166	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	46	theme	tetrasialylated	1152:1166	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	46	theme	tetrasialylated	1152:1166	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	9	47	theme	GOLD	1404:1407	arg1	stages					1409:1414	different COPD GOLD stages	1389:1414	different COPD GOLD stages	1389:1414	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	6	48	theme	Replication	839:849	arg1	cohort					851:856	Replication cohort	839:856	Replication cohort	839:856	Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre.					
30463578	2	49	theme	same	349:352	arg1	status					365:370	the same functional status	345:370	the same functional status	345:370	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	9	50	theme	structures	1469:1478	arg1	abundance					1436:1444	the relative abundance	1423:1444	the relative abundance of more complex glycan structures	1423:1478	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	6	51	with	subjects	874:881	arg1	COPD					888:891	COPD	888:891	COPD	888:891	Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre.					
30463578	4	52	theme	glycosylation	619:631	arg1	profiles					633:640	immunoglobulin G (IgG) glycosylation profiles	596:640	immunoglobulin G (IgG) glycosylation profiles	596:640	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	13	53	theme	potential	2048:2056	arg1	markers					2064:2070	potential novel markers	2048:2070	potential novel markers of the disease progression and severity	2048:2110	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	4	54	theme	G	611:611	arg1	profiles					633:640	immunoglobulin G (IgG) glycosylation profiles	596:640	immunoglobulin G (IgG) glycosylation profiles	596:640	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	7	55	theme	glycan	1097:1102	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	55	theme	glycan	1097:1102	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	2	56	theme	similar	290:296	arg1	parameters					310:319	similar spirometric parameters	290:319	similar spirometric parameters	290:319	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	1	57	theme	complex	168:174	arg1	disease					148:154	BACKGROUND Chronic obstructive pulmonary disease	107:154	BACKGROUND Chronic obstructive pulmonary disease (COPD)	107:161	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	57	theme	complex	168:174	arg1	condition					176:184	a complex condition	166:184	a complex condition	166:184	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	0	58	theme	immunoglobulin	48:61	arg1	G					63:63	immunoglobulin G	48:63	immunoglobulin G	48:63	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	8	59	from	decline	1238:1244	arg1	species					1275:1281	plasma monogalactosylated species	1249:1281	plasma monogalactosylated species	1249:1281	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	5	60	with	patients	781:788	arg1	COPD					795:798	COPD	795:798	COPD	795:798	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	8	61	theme	monogalactosylated	1256:1273	arg1	species					1275:1281	plasma monogalactosylated species	1249:1281	plasma monogalactosylated species	1249:1281	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	9	62	from	value	1353:1357	arg1	subjects					1377:1384	distinguishing subjects	1362:1384	distinguishing subjects	1362:1384	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	5	63	with	controls	816:823	arg1	COPD					795:798	COPD	795:798	COPD	795:798	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	5	64	theme	plasma	709:714	arg1	protein					716:722	total plasma protein	703:722	total plasma protein	703:722	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	13	65	theme	N-glycome	1996:2004	arg1	changes					2006:2012	plasma protein and IgG N-glycome changes	1973:2012	changes	2006:2012	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	10	66	theme	risk	1675:1678	arg1	factor					1680:1685	the major risk factor	1665:1685	the major risk factor for COPD development	1665:1706	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	10	66	theme	risk	1675:1678	arg1	smoking					1647:1653	smoking	1647:1653	smoking	1647:1653	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	1	67	theme	spirometric	212:222	arg1	assessment					224:233	spirometric assessment	212:233	spirometric assessment	212:233	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	8	68	theme	significant	1226:1236	arg1	decline					1238:1244	a significant decline	1224:1244	a significant decline in plasma monogalactosylated species	1224:1281	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	1	69	theme	obstructive	126:136	arg1	disease					148:154	BACKGROUND Chronic obstructive pulmonary disease	107:154	BACKGROUND Chronic obstructive pulmonary disease (COPD)	107:161	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	69	theme	obstructive	126:136	arg1	condition					176:184	a complex condition	166:184	a complex condition	166:184	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	69	theme	obstructive	126:136	arg1	COPD					157:160	COPD	157:160	COPD	157:160	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	6	70	theme	Croatian	931:938	arg1	centre					948:953	another Croatian medical centre	923:953	another Croatian medical centre	923:953	Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre.					
30463578	13	71	theme	new	1955:1957	arg1	insights					1959:1966	new insights	1955:1966	new insights into plasma protein and IgG N-glycome changes occurring in COPD	1955:2030	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	5	72	from	Croatia	830:836	arg1	total					768:772	the total	764:772	the total of 137 patients with COPD and 95 matching controls from Croatia	764:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	5	72	from	Croatia	830:836	arg1	patients					781:788	137 patients	777:788	137 patients with COPD	777:798	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	5	72	from	Croatia	830:836	arg1	controls					816:823	95 matching controls	804:823	95 matching controls from Croatia	804:836	METHODS Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia.					
30463578	0	73	theme	chronic	68:74	arg1	disease					98:104	chronic obstructive pulmonary disease	68:104	chronic obstructive pulmonary disease	68:104	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	8	74	from	change	1297:1302	arg1	species					1275:1281	plasma monogalactosylated species	1249:1281	plasma monogalactosylated species	1249:1281	We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome.					
30463578	7	75	from	N-glycome	979:987	arg1	subjects					997:1004	COPD subjects	992:1004	COPD subjects	992:1004	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	4	76	theme	COPD	518:521	arg1	biomarkers					523:532	COPD biomarkers	518:532	COPD biomarkers	518:532	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	0	77	theme	pulmonary	88:96	arg1	disease					98:104	chronic obstructive pulmonary disease	68:104	chronic obstructive pulmonary disease	68:104	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	0	78	from	patterns	16:23	arg1	disease					98:104	chronic obstructive pulmonary disease	68:104	chronic obstructive pulmonary disease	68:104	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	11	79	theme	glycans	1758:1764	arg1	complexity					1744:1753	complexity	1744:1753	complexity of glycans	1744:1764	CONCLUSIONS This study showed that complexity of glycans associates with COPD, mirroring also the disease severity.					
30463578	7	80	theme	protein	971:977	arg1	N-glycome					979:987	Plasma protein N-glycome	964:987	Plasma protein N-glycome in COPD subjects	964:1004	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	13	81	theme	progression	2087:2097	arg1	markers					2064:2070	potential novel markers	2048:2070	potential novel markers of the disease progression and severity	2048:2110	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	4	82	theme	N-glycans	505:513	arg1	value					496:500	the potential value	482:500	the potential value of N-glycans as COPD biomarkers	482:532	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	10	83	theme	COPD	1691:1694	arg1	development					1696:1706	COPD development	1691:1706	COPD development	1691:1706	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	13	84	theme	severity	2103:2110	arg1	markers					2064:2070	potential novel markers	2048:2070	potential novel markers of the disease progression and severity	2048:2110	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	3	85	dep	investigated	437:448	arg1	overcome					376:383	overcome	376:383	To overcome this limitation	373:399	To overcome this limitation novel biomarkers for COPD have been investigated.					
30463578	7	86	theme	fucosylated	1182:1192	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	86	theme	fucosylated	1182:1192	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	9	87	theme	distinguishing	1362:1375	arg1	subjects					1377:1384	distinguishing subjects	1362:1384	distinguishing subjects	1362:1384	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	1	88	theme	BACKGROUND	107:116	arg1	disease					148:154	BACKGROUND Chronic obstructive pulmonary disease	107:154	BACKGROUND Chronic obstructive pulmonary disease (COPD)	107:161	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	88	theme	BACKGROUND	107:116	arg1	condition					176:184	a complex condition	166:184	a complex condition	166:184	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	1	88	theme	BACKGROUND	107:116	arg1	COPD					157:160	COPD	157:160	COPD	157:160	BACKGROUND Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment.					
30463578	3	89	theme	novel	401:405	arg1	biomarkers					407:416	novel biomarkers	401:416	novel biomarkers for COPD	401:425	To overcome this limitation novel biomarkers for COPD have been investigated.					
30463578	4	90	theme	protein	584:590	arg1	variation					564:572	the individual variation	549:572	the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls	549:683	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	0	91	gly	N-glycosylation	0:14	arg1	proteins					35:42	plasma proteins	28:42	plasma proteins	28:42	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	0	91	gly	N-glycosylation	0:14	arg1	G					63:63	immunoglobulin G	48:63	immunoglobulin G	48:63	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	13	92	theme	novel	2058:2062	arg1	markers					2064:2070	potential novel markers	2048:2070	potential novel markers of the disease progression and severity	2048:2110	In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.					
30463578	0	93	theme	G	63:63	arg1	patterns					16:23	N-glycosylation patterns	0:23	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.	0:105	N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease.					
30463578	9	94	theme	COPD	1399:1402	arg1	stages					1409:1414	different COPD GOLD stages	1389:1414	different COPD GOLD stages	1389:1414	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30463578	6	95	with	controls	901:908	arg1	COPD					888:891	COPD	888:891	COPD	888:891	Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre.					
30463578	4	96	theme	individual	553:562	arg1	variation					564:572	the individual variation	549:572	the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls	549:683	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	2	97	theme	functional	354:363	arg1	status					365:370	the same functional status	345:370	the same functional status	345:370	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	2	98	contain	have	340:343	arg2	status					365:370	the same functional status	345:370	the same functional status	345:370	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	2	98	contain	have	340:343	arg1	subjects					276:283	subjects	276:283	subjects with similar spirometric parameters	276:319	However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status.					
30463578	12	99	theme	exacerbation	1871:1882	arg1	frequency					1884:1892	exacerbation frequency	1871:1892	exacerbation frequency	1871:1892	Moreover, changes in N-glycome associate with exacerbation frequency and are affected by smoking.					
30463578	10	100	theme	significant	1551:1561	arg1	associations					1563:1574	statistically significant associations	1537:1574	statistically significant associations	1537:1574	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	4	101	with	subjects	645:652	arg1	COPD					659:662	COPD	659:662	COPD	659:662	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	4	102	from	variation	564:572	arg1	controls					676:683	healthy controls	668:683	healthy controls	668:683	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	4	102	from	variation	564:572	arg1	subjects					645:652	subjects	645:652	subjects with COPD	645:662	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	4	103	theme	profiles	633:640	arg1	variation					564:572	the individual variation	549:572	the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls	549:683	Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls.					
30463578	10	104	theme	exacerbations	1598:1610	arg1	frequency					1585:1593	the frequency	1581:1593	the frequency of exacerbations	1581:1610	Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development.					
30463578	7	105	gly	fucosylated	1182:1192	arg1	glycoforms					1194:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms	1116:1203	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	7	105	gly	fucosylated	1182:1192	arg1	structures					1104:1113	more complex glycan structures	1084:1113	more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms)	1084:1204	RESULTS Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms).					
30463578	9	106	theme	relative	1427:1434	arg1	abundance					1436:1444	the relative abundance	1423:1444	the relative abundance of more complex glycan structures	1423:1478	N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed.					
30157878	5	0	dep	correlated	736:745	arg1	whereas					769:775	whereas	769:775	whereas	769:775	RESULTS Blood lipids were positively correlated with glycan peak GP6, whereas they were negatively correlated with GP18 (P < 0.05/57).					
30157878	8	1	theme	bisecting	1456:1464	arg1	GlcNAcs					1466:1472	bisecting GlcNAcs	1456:1472	bisecting GlcNAcs	1456:1472	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	1	2	theme	immunoglobulin	180:193	arg1	IgG					198:200	IgG	198:200	IgG	198:200	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	1	2	theme	immunoglobulin	180:193	arg1	G					195:195	immunoglobulin G	180:195	immunoglobulin G (IgG)	180:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	8	3	theme	acid	1423:1426	arg1	loss					1394:1397	the observed loss	1381:1397	the observed loss of galactose and sialic acid	1381:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	3	theme	acid	1423:1426	arg1	lipids					1370:1375	blood lipids	1364:1375	blood lipids	1364:1375	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	7	4	theme	IgG	1123:1125	arg1	patterns					1135:1142	IgG glycans patterns	1123:1142	IgG glycans patterns	1123:1142	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	6	5	theme	correlation	848:858	arg1	analysis					860:867	The canonical correlation analysis	834:867	The canonical correlation analysis	834:867	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	6	theme	blood	1003:1007	arg1	triglycerides					1052:1064	total triglycerides	1046:1064	total triglycerides (TG)	1046:1069	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	6	theme	blood	1003:1007	arg1	lipoprotein					1087:1097	low-density lipoprotein	1075:1097	low-density lipoprotein (r = 0.390, P < 0.001)	1075:1120	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	6	theme	blood	1003:1007	arg1	lipids					1009:1014	blood lipids	1003:1014	blood lipids	1003:1014	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	6	theme	blood	1003:1007	arg1	cholesterol					1033:1043	total cholesterol	1027:1043	total cholesterol	1027:1043	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	7	theme	canonical	838:846	arg1	correlation					848:858	The canonical correlation	834:858	The canonical correlation analysis	834:867	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	1	8	theme	pro-	263:266	arg1	functionality					294:306	pro- and anti-inflammatory IgG functionality	263:306	pro- and anti-inflammatory IgG functionality	263:306	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	1	9	from	consequences	164:175	arg1	IgG					198:200	IgG	198:200	IgG	198:200	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	1	9	from	consequences	164:175	arg1	G					195:195	immunoglobulin G	180:195	immunoglobulin G (IgG)	180:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	3	10	theme	cross-sectional	548:562	arg1	study					564:568	a cross-sectional study	546:568	a cross-sectional study including 598 Han Chinese aged 20-68 years	546:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	1	11	theme	functional	153:162	arg1	consequences					164:175	significant structural and functional consequences	126:175	significant structural and functional consequences on immunoglobulin G (IgG)	126:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	3	12	theme	glycosylation	491:503	arg1	patterns					475:482	patterns	475:482	patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years	475:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	8	13	dep	development	1548:1558	arg1	the					1544:1546	the	1544:1546	the	1544:1546	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	6	14	theme	low-density	1075:1085	arg1	r = 0.390					1100:1108	r = 0.390	1100:1108	r = 0.390	1100:1108	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	14	theme	low-density	1075:1085	arg1	lipoprotein					1087:1097	low-density lipoprotein	1075:1097	low-density lipoprotein (r = 0.390, P < 0.001)	1075:1120	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	8	15	theme	possible	1335:1342	arg1	association					1344:1354	a possible association	1333:1354	a possible association between blood lipids and the observed loss of galactose and sialic acid	1333:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	2	16	theme	type	404:407	arg1	diabetes					411:418	type 2 diabetes	404:418	type 2 diabetes	404:418	Studies have demonstrated that IgG N-glycosylation is associated with ageing, body mass index, type 2 diabetes and hypertension.					
30157878	0	17	from	N-glycosylation	32:46	arg1	dyslipidaemia					68:80	dyslipidaemia	68:80	dyslipidaemia	68:80	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	0	17	from	N-glycosylation	32:46	arg1	lipids					57:62	blood lipids	51:62	blood lipids	51:62	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	7	18	with	able	1149:1152	arg1	area					1224:1227	an area	1221:1227	an area under the curve of 0.692 (95% confidence interval 0.644-0.740)	1221:1290	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	4	19	theme	liquid	676:681	arg1	chromatography					683:696	ultra-performance liquid chromatography	658:696	ultra-performance liquid chromatography	658:696	The IgG glycome composition was analysed by ultra-performance liquid chromatography.					
30157878	7	20	theme	confidence	1259:1268	arg1	interval					1270:1277	95% confidence interval 0.644-0.740	1255:1289	95% confidence interval 0.644-0.740	1255:1289	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	7	20	theme	confidence	1259:1268	arg1	0.692					1248:1252	0.692	1248:1252	0.692	1248:1252	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	8	21	theme	chronic	1505:1511	arg1	inflammation					1513:1524	the chronic inflammation	1501:1524	the chronic inflammation accompanying with the development and procession of dyslipidaemia	1501:1590	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	1	22	theme	BACKGROUND	83:92	arg1	N-glycosylation					106:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	5	23	dep	RESULTS	699:705	arg1	correlated					736:745	correlated	736:745	were positively correlated with glycan peak GP6	720:766	RESULTS Blood lipids were positively correlated with glycan peak GP6, whereas they were negatively correlated with GP18 (P < 0.05/57).					
30157878	1	24	theme	anti-inflammatory	272:288	arg1	functionality					294:306	pro- and anti-inflammatory IgG functionality	263:306	pro- and anti-inflammatory IgG functionality	263:306	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	1	25	theme	Alternative	94:104	arg1	N-glycosylation					106:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	2	26	theme	mass	392:395	arg1	index					397:401	body mass index	387:401	body mass index	387:401	Studies have demonstrated that IgG N-glycosylation is associated with ageing, body mass index, type 2 diabetes and hypertension.					
30157878	0	27	theme	immunoglobulin	15:28	arg1	G					30:30	immunoglobulin G	15:30	immunoglobulin G	15:30	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	1	28	theme	IgG	290:292	arg1	functionality					294:306	pro- and anti-inflammatory IgG functionality	263:306	pro- and anti-inflammatory IgG functionality	263:306	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	6	29	dep	r = 0.390	1100:1108	arg1	P < 0.001					1111:1119	P < 0.001	1111:1119	P < 0.001	1111:1119	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	30	theme	total	1046:1050	arg1	triglycerides					1052:1064	total triglycerides	1046:1064	total triglycerides (TG)	1046:1069	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	30	theme	total	1046:1050	arg1	TG					1067:1068	TG	1067:1068	TG	1067:1068	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	3	31	theme	Han	584:586	arg1	20-68 years					601:611	598 Han Chinese aged 20-68 years	580:611	598 Han Chinese aged 20-68 years	580:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	8	32	theme	blood	1364:1368	arg1	lipids					1370:1375	blood lipids	1364:1375	blood lipids	1364:1375	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	33	dep	CONCLUSIONS	1293:1303	arg1	indicated					1318:1326	indicated	1318:1326	indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia	1318:1590	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	7	34	theme	%	1257:1257	arg1	interval					1270:1277	95% confidence interval 0.644-0.740	1255:1289	95% confidence interval 0.644-0.740	1255:1289	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	7	34	theme	%	1257:1257	arg1	0.692					1248:1252	0.692	1248:1252	0.692	1248:1252	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	5	35	theme	glycan	752:757	arg1	GP6					764:766	glycan peak GP6	752:766	glycan peak GP6	752:766	RESULTS Blood lipids were positively correlated with glycan peak GP6, whereas they were negatively correlated with GP18 (P < 0.05/57).					
30157878	4	36	theme	ultra-performance	658:674	arg1	chromatography					683:696	ultra-performance liquid chromatography	658:696	ultra-performance liquid chromatography	658:696	The IgG glycome composition was analysed by ultra-performance liquid chromatography.					
30157878	1	37	theme	immune	218:223	arg1	responses					225:233	immune responses	218:233	immune responses	218:233	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	0	38	theme	G	30:30	arg1	changes					4:10	The changes	0:10	The changes of immunoglobulin G	0:30	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	1	39	contain	has	122:124	arg1	N-glycosylation					106:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation	83:120	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	1	39	contain	has	122:124	arg2	consequences					164:175	significant structural and functional consequences	126:175	significant structural and functional consequences on immunoglobulin G (IgG)	126:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	5	40	theme	peak	759:762	arg1	GP6					764:766	glycan peak GP6	752:766	glycan peak GP6	752:766	RESULTS Blood lipids were positively correlated with glycan peak GP6, whereas they were negatively correlated with GP18 (P < 0.05/57).					
30157878	3	41	dep	METHODS	438:444	arg1	demonstrated					462:473	demonstrated	462:473	have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years	457:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	8	42	dep	indicated	1318:1326	arg1	addition					1444:1451	the addition	1440:1451	the addition of bisecting GlcNAcs	1440:1472	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	42	dep	indicated	1318:1326	arg1	related					1490:1496	related	1490:1496	related	1490:1496	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	42	dep	indicated	1318:1326	arg1	association					1344:1354	a possible association	1333:1354	a possible association between blood lipids and the observed loss of galactose and sialic acid	1333:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	0	43	theme	blood	51:55	arg1	lipids					57:62	blood lipids	51:62	blood lipids	51:62	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	5	44	theme	Blood	707:711	arg1	lipids					713:718	Blood lipids	707:718	Blood lipids	707:718	RESULTS Blood lipids were positively correlated with glycan peak GP6, whereas they were negatively correlated with GP18 (P < 0.05/57).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP14					941:944	GP14	941:944	GP14	941:944	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP23					962:965	GP23	962:965	GP23	962:965	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP4					923:925	GP4	923:925	GP4	923:925	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP6					928:930	GP6	928:930	GP6	928:930	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP17					947:950	GP17	947:950	GP17	947:950	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP18					953:956	GP18	953:956	GP18	953:956	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	structures					901:910	initial N-glycan structures	884:910	initial N-glycan structures	884:910	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	45	theme	N-glycan	892:899	arg1	GP9-12					933:938	GP9-12	933:938	GP9-12	933:938	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	3	46	theme	Chinese	588:594	arg1	20-68 years					601:611	598 Han Chinese aged 20-68 years	580:611	598 Han Chinese aged 20-68 years	580:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	1	47	theme	significant	126:136	arg1	consequences					164:175	significant structural and functional consequences	126:175	significant structural and functional consequences on immunoglobulin G (IgG)	126:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	6	48	theme	initial	884:890	arg1	GP14					941:944	GP14	941:944	GP14	941:944	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP23					962:965	GP23	962:965	GP23	962:965	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP4					923:925	GP4	923:925	GP4	923:925	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP6					928:930	GP6	928:930	GP6	928:930	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP17					947:950	GP17	947:950	GP17	947:950	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP18					953:956	GP18	953:956	GP18	953:956	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	structures					901:910	initial N-glycan structures	884:910	initial N-glycan structures	884:910	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	6	48	theme	initial	884:890	arg1	GP9-12					933:938	GP9-12	933:938	GP9-12	933:938	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	3	49	theme	aged	596:599	arg1	20-68 years					601:611	598 Han Chinese aged 20-68 years	580:611	598 Han Chinese aged 20-68 years	580:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	1	50	theme	structural	138:147	arg1	consequences					164:175	significant structural and functional consequences	126:175	significant structural and functional consequences on immunoglobulin G (IgG)	126:201	BACKGROUND Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionality.					
30157878	8	51	theme	galactose	1402:1410	arg1	loss					1394:1397	the observed loss	1381:1397	the observed loss of galactose and sialic acid	1381:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	51	theme	galactose	1402:1410	arg1	lipids					1370:1375	blood lipids	1364:1375	blood lipids	1364:1375	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	7	52	theme	0.692	1248:1252	arg1	curve					1239:1243	the curve	1235:1243	the curve of 0.692 (95% confidence interval 0.644-0.740)	1235:1290	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	7	53	with	patients	1169:1176	arg1	dyslipidaemia					1183:1195	dyslipidaemia	1183:1195	dyslipidaemia	1183:1195	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	4	54	theme	IgG	618:620	arg1	composition					630:640	The IgG glycome composition	614:640	The IgG glycome composition	614:640	The IgG glycome composition was analysed by ultra-performance liquid chromatography.					
30157878	0	55	gly	N-glycosylation	32:46	arg1	dyslipidaemia					68:80	dyslipidaemia	68:80	dyslipidaemia	68:80	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	0	55	gly	N-glycosylation	32:46	arg1	lipids					57:62	blood lipids	51:62	blood lipids	51:62	The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia.					
30157878	2	56	theme	IgG	340:342	arg1	N-glycosylation					344:358	IgG N-glycosylation	340:358	IgG N-glycosylation	340:358	Studies have demonstrated that IgG N-glycosylation is associated with ageing, body mass index, type 2 diabetes and hypertension.					
30157878	6	57	theme	total	1027:1031	arg1	cholesterol					1033:1043	total cholesterol	1027:1043	total cholesterol	1027:1043	The canonical correlation analysis indicated that initial N-glycan structures, including GP4, GP6, GP9-12, GP14, GP17, GP18 and GP23, were significantly correlated with blood lipids, including total cholesterol, total triglycerides (TG) and low-density lipoprotein (r = 0.390, P < 0.001).					
30157878	8	58	theme	observed	1385:1392	arg1	loss					1394:1397	the observed loss	1381:1397	the observed loss of galactose and sialic acid	1381:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	59	theme	sialic	1416:1421	arg1	acid					1423:1426	sialic acid	1416:1426	sialic acid	1416:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	2	60	theme	body	387:390	arg1	index					397:401	body mass index	387:401	body mass index	387:401	Studies have demonstrated that IgG N-glycosylation is associated with ageing, body mass index, type 2 diabetes and hypertension.					
30157878	3	61	theme	IgG	487:489	arg1	glycosylation					491:503	IgG glycosylation	487:503	IgG glycosylation	487:503	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	7	62	theme	glycans	1127:1133	arg1	patterns					1135:1142	IgG glycans patterns	1123:1142	IgG glycans patterns	1123:1142	IgG glycans patterns were able to distinguish patients with dyslipidaemia from the controls, with an area under the curve of 0.692 (95% confidence interval 0.644-0.740).					
30157878	8	63	theme	GlcNAcs	1466:1472	arg1	addition					1444:1451	the addition	1440:1451	the addition of bisecting GlcNAcs	1440:1472	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	63	theme	GlcNAcs	1466:1472	arg1	association					1344:1354	a possible association	1333:1354	a possible association between blood lipids and the observed loss of galactose and sialic acid	1333:1426	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	64	theme	dyslipidaemia	1578:1590	arg1	procession					1564:1573	procession	1564:1573	procession	1564:1573	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	8	64	theme	dyslipidaemia	1578:1590	arg1	development					1548:1558	development	1548:1558	development	1548:1558	CONCLUSIONS Our findings indicated that a possible association between blood lipids and the observed loss of galactose and sialic acid, as well as the addition of bisecting GlcNAcs, which might be related to the chronic inflammation accompanying with the development and procession of dyslipidaemia.					
30157878	3	65	theme	blood	530:534	arg1	lipids					536:541	blood lipids	530:541	blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years	530:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	3	66	from	lipids	536:541	arg1	study					564:568	a cross-sectional study	546:568	a cross-sectional study including 598 Han Chinese aged 20-68 years	546:611	METHODS Herein, we have demonstrated patterns of IgG glycosylation that are associated with blood lipids in a cross-sectional study including 598 Han Chinese aged 20-68 years.					
30157878	4	67	theme	glycome	622:628	arg1	composition					630:640	The IgG glycome composition	614:640	The IgG glycome composition	614:640	The IgG glycome composition was analysed by ultra-performance liquid chromatography.					
30553446	8	0	theme	IgA	1008:1010	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	4	1	from	structure	463:471	arg1	region					486:491	the hinge region	476:491	the hinge region (HR) of serum IgA	476:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	1	from	structure	463:471	arg1	HR					494:495	HR	494:495	HR	494:495	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	9	2	theme	Gal/GalNAc	1238:1247	arg1	ratio					1249:1253	the Gal/GalNAc ratio	1234:1253	the Gal/GalNAc ratio in the HR of the IgAN recipients	1234:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	8	3	theme	IgAN	1017:1020	arg1	n = 26					1034:1039	n = 26	1034:1039	n = 26	1034:1039	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	3	theme	IgAN	1017:1020	arg1	recipients					1022:1031	IgAN recipients	1017:1031	IgAN recipients (n = 26)	1017:1040	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	10	4	theme	Gal/GalNAc	1377:1386	arg1	ratio					1388:1392	The decreased Gal/GalNAc ratio	1363:1392	The decreased Gal/GalNAc ratio in IgAN recipients	1363:1411	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	6	5	theme	renal	699:703	arg1	donors					716:721	living renal transplant donors	692:721	living renal transplant donors	692:721	Herein, we investigated the prevalence of IgAD in living renal transplant donors in our cohort.					
30553446	2	6	theme	Asymptomatic	254:265	arg1	deposition					271:280	Asymptomatic IgA deposition	254:280	Asymptomatic IgA deposition (IgAD)	254:287	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	2	6	theme	Asymptomatic	254:265	arg1	IgAD					283:286	IgAD	283:286	IgAD	283:286	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	1	7	theme	glomerulonephritis	155:172	arg1	nephropathy					101:111	IgA nephropathy	97:111	IgA nephropathy (IgAN)	97:118	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	1	7	theme	glomerulonephritis	155:172	arg1	form					139:142	the most common form	123:142	the most common form of primary glomerulonephritis	123:172	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	8	8	from	donors	1048:1053	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	8	from	donors	1048:1053	arg1	IgA					1008:1010	serum IgA	1002:1010	serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	1002:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	9	theme	helthy	1078:1083	arg1	n = 27					1093:1098	n = 27	1093:1098	n = 27	1093:1098	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	9	theme	helthy	1078:1083	arg1	donors					1085:1090	non-IgAD helthy donors	1069:1090	non-IgAD helthy donors (n = 27)	1069:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	10	theme	IgAD	1043:1046	arg1	n = 17					1056:1061	n = 17	1056:1061	n = 17	1056:1061	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	10	theme	IgAD	1043:1046	arg1	donors					1048:1053	IgAD donors	1043:1053	IgAD donors (n = 17)	1043:1062	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	4	11	theme	key	554:556	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	9	12	theme	recipients	1277:1286	arg1	HR					1262:1263	the HR	1258:1263	the HR of the IgAN recipients	1258:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	10	13	theme	galactose-deficient	1442:1460	arg1	IgA1					1462:1465	galactose-deficient IgA1	1442:1465	galactose-deficient IgA1	1442:1465	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	12	14	theme	material	1738:1745	arg1	role					1758:1761	a material pathogenic role	1736:1761	a material pathogenic role	1736:1761	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	8	15	theme	matrix-assisted	1107:1121	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	15	theme	matrix-assisted	1107:1121	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	16	theme	desorption	1129:1138	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	16	theme	desorption	1129:1138	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	5	17	theme	O-glycan	605:612	arg1	structure					614:622	IgA1 O-glycan structure	600:622	IgA1 O-glycan structure in IgAD patients	600:639	However, little is known about IgA1 O-glycan structure in IgAD patients.					
30553446	8	18	theme	time-of-flight	1151:1164	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	18	theme	time-of-flight	1151:1164	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	11	19	theme	O-glycan	1538:1545	arg1	structures					1547:1556	the O-glycan structures	1534:1556	the O-glycan structures in IgAN recipients and IgAD donors	1534:1591	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	1	20	theme	common	132:137	arg1	nephropathy					101:111	IgA nephropathy	97:111	IgA nephropathy (IgAN)	97:118	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	1	20	theme	common	132:137	arg1	form					139:142	the most common form	123:142	the most common form of primary glomerulonephritis	123:172	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	4	21	theme	IgA1	449:452	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	21	theme	IgA1	449:452	arg1	structure					463:471	aberrant IgA1 O-glycan structure	440:471	aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA	440:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	21	theme	IgA1	449:452	arg1	one					527:529	one	527:529	one	527:529	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	11	22	theme	IgAD	1581:1584	arg1	donors					1586:1591	IgAD donors	1581:1591	IgAD donors	1581:1591	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	0	23	theme	nephropathy	73:83	arg1	recipients					85:94	IgA nephropathy recipients	69:94	IgA nephropathy recipients	69:94	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	12	24	theme	Gal	1706:1708	arg1	contents					1710:1717	decreased GalNAc and Gal contents	1685:1717	decreased GalNAc and Gal contents in HR	1685:1723	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	9	25	theme	healthy	1347:1353	arg1	donors					1355:1360	the IgAD and non-IgAD healthy donors	1325:1360	donors	1355:1360	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	0	26	from	Difference	0:9	arg1	O-glycosylation					19:33	IgA1 O-glycosylation	14:33	IgA1 O-glycosylation	14:33	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	11	27	from	donors	1668:1673	arg1	common					1645:1650	common	1645:1650	common	1645:1650	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	8	28	theme	MALDI-TOF	1185:1193	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	28	theme	MALDI-TOF	1185:1193	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	7	29	theme	blood	838:842	arg1	relationship					844:855	statistically significant blood relationship	812:855	statistically significant blood relationship	812:855	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	11	30	from	common	1645:1650	arg1	donors					1668:1673	IgAN related donors	1655:1673	IgAN related donors	1655:1673	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	12	31	theme	GalNAc	1695:1700	arg1	contents					1710:1717	decreased GalNAc and Gal contents	1685:1717	decreased GalNAc and Gal contents in HR	1685:1723	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	1	32	theme	IgA	97:99	arg1	form					139:142	the most common form	123:142	the most common form of primary glomerulonephritis	123:172	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	1	32	theme	IgA	97:99	arg1	IgAN					114:117	IgAN	114:117	IgAN	114:117	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	1	32	theme	IgA	97:99	arg1	nephropathy					101:111	IgA nephropathy	97:111	IgA nephropathy (IgAN)	97:118	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	0	33	theme	IgA1	14:17	arg1	O-glycosylation					19:33	IgA1 O-glycosylation	14:33	IgA1 O-glycosylation	14:33	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	11	34	theme	first	1510:1514	arg1	this					1498:1501	this	1498:1501	this	1498:1501	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	11	34	theme	first	1510:1514	arg1	report					1516:1521	the first report	1506:1521	the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors	1506:1673	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	7	35	dep	%	883:883	arg1	p = 0.0073					939:948	p = 0.0073	939:948	p = 0.0073	939:948	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	0	36	theme	deposition	47:56	arg1	donors					58:63	IgA deposition donors	43:63	IgA deposition donors	43:63	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	10	37	from	ratio	1388:1392	arg1	recipients					1402:1411	IgAN recipients	1397:1411	IgAN recipients	1397:1411	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	9	38	theme	GalNAc	1215:1220	arg1	ratio					1249:1253	the Gal/GalNAc ratio	1234:1253	the Gal/GalNAc ratio in the HR of the IgAN recipients	1234:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	9	38	theme	GalNAc	1215:1220	arg1	numbers					1204:1210	The numbers	1200:1210	The numbers of GalNAc and Gal	1200:1228	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	8	39	theme	IgA1	975:978	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	6	40	from	prevalence	670:679	arg1	donors					716:721	living renal transplant donors	692:721	living renal transplant donors	692:721	Herein, we investigated the prevalence of IgAD in living renal transplant donors in our cohort.					
30553446	10	41	theme	decreased	1367:1375	arg1	ratio					1388:1392	The decreased Gal/GalNAc ratio	1363:1392	The decreased Gal/GalNAc ratio in IgAN recipients	1363:1411	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	7	42	theme	renal	779:783	arg1	donors					796:801	161 renal transplant donors	775:801	161 renal transplant donors	775:801	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	9	43	theme	Gal	1226:1228	arg1	ratio					1249:1253	the Gal/GalNAc ratio	1234:1253	the Gal/GalNAc ratio in the HR of the IgAN recipients	1234:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	9	43	theme	Gal	1226:1228	arg1	numbers					1204:1210	The numbers	1200:1210	The numbers of GalNAc and Gal	1200:1228	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	10	44	theme	increased	1423:1431	arg1	ratio					1433:1437	the increased ratio	1419:1437	the increased ratio of galactose-deficient IgA1	1419:1465	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	11	45	theme	related	1660:1666	arg1	donors					1668:1673	IgAN related donors	1655:1673	IgAN related donors	1655:1673	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	8	46	theme	serum	1002:1006	arg1	IgA					1008:1010	serum IgA	1002:1010	serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	1002:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	4	47	theme	IgA	507:509	arg1	region					486:491	the hinge region	476:491	the hinge region (HR) of serum IgA	476:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	47	theme	IgA	507:509	arg1	HR					494:495	HR	494:495	HR	494:495	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	10	48	theme	IgAN	1397:1400	arg1	recipients					1402:1411	IgAN recipients	1397:1411	IgAN recipients	1397:1411	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	5	49	from	structure	614:622	arg1	patients					632:639	IgAD patients	627:639	IgAD patients	627:639	However, little is known about IgA1 O-glycan structure in IgAD patients.					
30553446	6	50	theme	transplant	705:714	arg1	donors					716:721	living renal transplant donors	692:721	living renal transplant donors	692:721	Herein, we investigated the prevalence of IgAD in living renal transplant donors in our cohort.					
30553446	2	51	theme	IgA	267:269	arg1	deposition					271:280	Asymptomatic IgA deposition	254:280	Asymptomatic IgA deposition (IgAD)	254:287	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	2	51	theme	IgA	267:269	arg1	IgAD					283:286	IgAD	283:286	IgAD	283:286	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	1	52	theme	primary	147:153	arg1	glomerulonephritis					155:172	primary glomerulonephritis	147:172	primary glomerulonephritis	147:172	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	7	53	contain	have	807:810	arg1	IgAD					738:741	IgAD	738:741	IgAD	738:741	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	53	contain	have	807:810	arg2	relationship					844:855	statistically significant blood relationship	812:855	statistically significant blood relationship	812:855	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	6	54	theme	living	692:697	arg1	donors					716:721	living renal transplant donors	692:721	living renal transplant donors	692:721	Herein, we investigated the prevalence of IgAD in living renal transplant donors in our cohort.					
30553446	12	55	theme	pathogenic	1747:1756	arg1	role					1758:1761	a material pathogenic role	1736:1761	a material pathogenic role	1736:1761	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	8	56	theme	non-IgAD	1069:1076	arg1	n = 27					1093:1098	n = 27	1093:1098	n = 27	1093:1098	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	56	theme	non-IgAD	1069:1076	arg1	donors					1085:1090	non-IgAD helthy donors	1069:1090	non-IgAD helthy donors (n = 27)	1069:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	6	57	theme	IgAD	684:687	arg1	prevalence					670:679	the prevalence	666:679	the prevalence of IgAD in living renal transplant donors	666:721	Herein, we investigated the prevalence of IgAD in living renal transplant donors in our cohort.					
30553446	9	58	theme	IgAN	1272:1275	arg1	recipients					1277:1286	the IgAN recipients	1268:1286	the IgAN recipients	1268:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	4	59	theme	convincing	543:552	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	10	60	theme	IgA1	1462:1465	arg1	ratio					1433:1437	the increased ratio	1419:1437	the increased ratio of galactose-deficient IgA1	1419:1465	The decreased Gal/GalNAc ratio in IgAN recipients means the increased ratio of galactose-deficient IgA1.					
30553446	2	61	theme	donated	317:323	arg1	kidney					325:330	donated kidney	317:330	donated kidney	317:330	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	5	62	theme	IgAD	627:630	arg1	patients					632:639	IgAD patients	627:639	IgAD patients	627:639	However, little is known about IgA1 O-glycan structure in IgAD patients.					
30553446	7	63	from	%	883:883	arg1	relatives					888:896	relatives	888:896	relatives	888:896	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	4	64	theme	mediators	558:566	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	64	theme	mediators	558:566	arg1	structure					463:471	aberrant IgA1 O-glycan structure	440:471	aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA	440:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	64	theme	mediators	558:566	arg1	one					527:529	one	527:529	one	527:529	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	11	65	from	structures	1547:1556	arg1	recipients					1566:1575	IgAN recipients	1561:1575	IgAN recipients	1561:1575	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	11	65	from	structures	1547:1556	arg1	donors					1586:1591	IgAD donors	1581:1591	IgAD donors	1581:1591	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	5	66	theme	IgA1	600:603	arg1	structure					614:622	IgA1 O-glycan structure	600:622	IgA1 O-glycan structure in IgAD patients	600:639	However, little is known about IgA1 O-glycan structure in IgAD patients.					
30553446	8	67	theme	laser	1123:1127	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	67	theme	laser	1123:1127	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	11	68	theme	IgAN	1561:1564	arg1	recipients					1566:1575	IgAN recipients	1561:1575	IgAN recipients	1561:1575	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	0	69	theme	IgA	69:71	arg1	recipients					85:94	IgA nephropathy recipients	69:94	IgA nephropathy recipients	69:94	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	2	70	theme	other	241:245	arg1	hands					247:251	the other hands	237:251	the other hands	237:251	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	1	71	theme	disease	179:185	arg1	recurrence					187:196	disease recurrence	179:196	disease recurrence	179:196	IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis, and disease recurrence often occurs after transplantation.					
30553446	12	72	from	contents	1710:1717	arg1	HR					1722:1723	HR	1722:1723	HR	1722:1723	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	4	73	theme	O-glycan	454:461	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	73	theme	O-glycan	454:461	arg1	structure					463:471	aberrant IgA1 O-glycan structure	440:471	aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA	440:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	73	theme	O-glycan	454:461	arg1	one					527:529	one	527:529	one	527:529	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	8	74	theme	ionization	1140:1149	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	74	theme	ionization	1140:1149	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	9	75	from	numbers	1204:1210	arg1	HR					1262:1263	the HR	1258:1263	the HR of the IgAN recipients	1258:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	8	76	from	donors	1085:1090	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	76	from	donors	1085:1090	arg1	IgA					1008:1010	serum IgA	1002:1010	serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	1002:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	9	77	theme	non-IgAD	1338:1345	arg1	donors					1355:1360	the IgAD and non-IgAD healthy donors	1325:1360	donors	1355:1360	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	8	78	theme	mass	1166:1169	arg1	MS					1195:1196	MALDI-TOF MS	1185:1196	MALDI-TOF MS	1185:1196	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	78	theme	mass	1166:1169	arg1	spectrometry					1171:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	1107:1182	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1107:1197	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	7	79	theme	IgAN	862:865	arg1	recipients					867:876	IgAN recipients	862:876	IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073)	862:949	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	79	theme	IgAN	862:865	arg1	%					883:883	28.6%	879:883	28.6% in relatives	879:896	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	79	theme	IgAN	862:865	arg1	%					905:905	9.8%	902:905	9.8% in non-relatives	902:922	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	12	80	theme	decreased	1685:1693	arg1	contents					1710:1717	decreased GalNAc and Gal contents	1685:1717	decreased GalNAc and Gal contents in HR	1685:1723	Overall, decreased GalNAc and Gal contents in HR could play a material pathogenic role in IgAN.					
30553446	2	81	located	observed	305:312	arg1	kidney					325:330	donated kidney	317:330	donated kidney	317:330	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	2	81	located	observed	305:312	arg1	hands					247:251	the other hands	237:251	the other hands	237:251	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	2	81	located	observed	305:312	arg2	IgAD					283:286	IgAD	283:286	IgAD	283:286	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	2	81	located	observed	305:312	arg2	deposition					271:280	Asymptomatic IgA deposition	254:280	Asymptomatic IgA deposition (IgAD)	254:287	On the other hands, Asymptomatic IgA deposition (IgAD) is occasionally observed in donated kidney.					
30553446	7	82	theme	significant	826:836	arg1	relationship					844:855	statistically significant blood relationship	812:855	statistically significant blood relationship	812:855	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	9	83	from	ratio	1249:1253	arg1	HR					1262:1263	the HR	1258:1263	the HR of the IgAN recipients	1258:1286	The numbers of GalNAc and Gal and the Gal/GalNAc ratio in the HR of the IgAN recipients had significantly lower comparing to the IgAD and non-IgAD healthy donors.					
30553446	4	84	theme	serum	501:505	arg1	IgA					507:509	serum IgA	501:509	serum IgA	501:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	0	85	theme	IgA	43:45	arg1	donors					58:63	IgA deposition donors	43:63	IgA deposition donors	43:63	Difference in IgA1 O-glycosylation between IgA deposition donors and IgA nephropathy recipients.					
30553446	4	86	theme	hinge	480:484	arg1	region					486:491	the hinge region	476:491	the hinge region (HR) of serum IgA	476:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	4	86	theme	hinge	480:484	arg1	HR					494:495	HR	494:495	HR	494:495	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	8	87	from	structure	989:997	arg1	recipients					1022:1031	IgAN recipients	1017:1031	IgAN recipients (n = 26)	1017:1040	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	87	from	structure	989:997	arg1	donors					1048:1053	IgAD donors	1043:1053	IgAD donors (n = 17)	1043:1062	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	87	from	structure	989:997	arg1	n = 17					1056:1061	n = 17	1056:1061	n = 17	1056:1061	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	87	from	structure	989:997	arg1	donors					1085:1090	non-IgAD helthy donors	1069:1090	non-IgAD helthy donors (n = 27)	1069:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	87	from	structure	989:997	arg1	n = 26					1034:1039	n = 26	1034:1039	n = 26	1034:1039	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	87	from	structure	989:997	arg1	n = 27					1093:1098	n = 27	1093:1098	n = 27	1093:1098	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	4	88	theme	aberrant	440:447	arg1	IgA1					449:452	aberrant IgA1	440:452	aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA	440:509	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
30553446	11	89	theme	IgAN	1655:1658	arg1	donors					1668:1673	IgAN related donors	1655:1673	IgAN related donors	1655:1673	To the best of our knowledge, this is the first report to compare the O-glycan structures in IgAN recipients and IgAD donors using MALDI-TOF MS. We concluded that IgAD was more common in IgAN related donors.					
30553446	7	90	theme	transplant	785:794	arg1	donors					796:801	161 renal transplant donors	775:801	161 renal transplant donors	775:801	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	8	91	theme	O-glycan	980:987	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	92	from	recipients	1022:1031	arg1	structure					989:997	the IgA1 O-glycan structure	971:997	the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	971:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	8	92	from	recipients	1022:1031	arg1	IgA					1008:1010	serum IgA	1002:1010	serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27)	1002:1099	Next, we evaluated the IgA1 O-glycan structure of serum IgA from IgAN recipients (n = 26), IgAD donors (n = 17), and non-IgAD helthy donors (n = 27) using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).					
30553446	7	93	from	%	905:905	arg1	non-relatives					910:922	non-relatives	910:922	non-relatives	910:922	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	94	located	observed	747:754	arg1	21					759:760	21	759:760	21	759:760	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	94	located	observed	747:754	arg1	%					766:766	13.0%	762:766	13.0%	762:766	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	7	94	located	observed	747:754	arg2	IgAD					738:741	IgAD	738:741	IgAD	738:741	IgAD was observed in 21(13.0%) among 161 renal transplant donors and have statistically significant blood relationship with IgAN recipients (28.6% in relatives vs. 9.8% in non-relatives, respectively; p = 0.0073).					
30553446	4	95	theme	most	538:541	arg1	mediators					558:566	the most convincing key mediators	534:566	the most convincing key mediators	534:566	In IgAN, aberrant IgA1 O-glycan structure in the hinge region (HR) of serum IgA is suggested as one of the most convincing key mediators.					
31464438	0	0	theme	Meat	71:74	arg1	Adulteration					76:87	Meat Adulteration	71:87	Meat Adulteration	71:87	N-Glycan Profile as a Tool in Qualitative and Quantitative Analysis of Meat Adulteration.					
31464438	3	1	gly	N-glycosylation	389:403	arg1	meats					427:431	different meats	417:431	different meats	417:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	2	2	theme	existing	333:340	arg1	methods					342:348	the existing methods	329:348	the existing methods	329:348	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	4	3	theme	meat	553:556	arg1	species					558:564	the investigated five meat species	531:564	the investigated five meat species (beef, chicken, pork, duck, and mutton)	531:604	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	6	4	theme	detailed	1007:1014	arg1	structures					1016:1025	the detailed structures	1003:1025	the detailed structures	1003:1025	The most distinguishing N-glycans from beef and duck were elucidated for the detailed structures.					
31464438	3	5	theme	meat	479:482	arg1	authentication					484:497	meat authentication	479:497	meat authentication	479:497	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	2	6	theme	sensitive	203:211	arg1	methods					213:219	novel robust and sensitive methods	186:219	novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods	186:348	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	2	7	theme	qualitative	265:275	arg1	means					294:298	qualitative and quantitative means	265:298	qualitative and quantitative means	265:298	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	0	8	theme	Adulteration	76:87	arg1	Analysis					59:66	Qualitative and Quantitative Analysis	30:66	Qualitative and Quantitative Analysis of Meat Adulteration	30:87	N-Glycan Profile as a Tool in Qualitative and Quantitative Analysis of Meat Adulteration.					
31464438	4	9	theme	principal	630:638	arg1	analysis					650:657	principal component analysis	630:657	principal component analysis	630:657	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	10	theme	adulteration	776:787	arg1	ratio					789:793	adulteration ratio	776:793	adulteration ratio	776:793	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	1	11	dep	meat	106:109	arg1	products					120:127	products	120:127	products	120:127	Adulteration of meat and meat products causes concerns to consumers.					
31464438	3	12	theme	different	417:425	arg1	meats					427:431	different meats	417:431	different meats	417:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	13	theme	meats	427:431	arg1	species					437:443	species	437:443	species	437:443	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	13	theme	meats	427:431	arg1	profiles					405:412	the protein N-glycosylation profiles	377:412	the protein N-glycosylation profiles of different meats	377:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	13	theme	meats	427:431	arg1	specific					445:452	specific	445:452	specific	445:452	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	0	14	from	Tool	22:25	arg1	Analysis					59:66	Qualitative and Quantitative Analysis	30:66	Qualitative and Quantitative Analysis of Meat Adulteration	30:87	N-Glycan Profile as a Tool in Qualitative and Quantitative Analysis of Meat Adulteration.					
31464438	4	15	theme	ratio	789:793	arg1	prediction					762:771	the prediction	758:771	the prediction of adulteration ratio	758:793	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	6	16	from	beef	969:972	arg1	N-glycans					954:962	The most distinguishing N-glycans	930:962	The most distinguishing N-glycans from beef and duck	930:981	The most distinguishing N-glycans from beef and duck were elucidated for the detailed structures.					
31464438	4	17	theme	partial	664:670	arg1	regression					685:694	partial least square regression	664:694	partial least square regression	664:694	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	18	theme	validation	737:746	arg1	model					748:752	a calibration and validation model	719:752	a calibration and validation model for the prediction of adulteration ratio	719:793	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	2	19	theme	quantitative	281:292	arg1	means					294:298	qualitative and quantitative means	265:298	qualitative and quantitative means	265:298	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	6	20	from	duck	978:981	arg1	N-glycans					954:962	The most distinguishing N-glycans	930:962	The most distinguishing N-glycans from beef and duck	930:981	The most distinguishing N-glycans from beef and duck were elucidated for the detailed structures.					
31464438	4	21	theme	investigated	535:546	arg1	species					558:564	the investigated five meat species	531:564	the investigated five meat species (beef, chicken, pork, duck, and mutton)	531:604	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	2	22	theme	meat	257:260	arg1	origin					247:252	the origin	243:252	the origin of meat	243:260	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	3	23	theme	protein	381:387	arg1	species					437:443	species	437:443	species	437:443	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	23	theme	protein	381:387	arg1	profiles					405:412	the protein N-glycosylation profiles	377:412	the protein N-glycosylation profiles of different meats	377:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	23	theme	protein	381:387	arg1	specific					445:452	specific	445:452	specific	445:452	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	5	24	theme	lower-value	847:857	arg1	meat					864:867	a lower-value duck meat	845:867	a lower-value duck meat	845:867	Using this method, beef samples adulterated with a lower-value duck meat could be detected down to the addition ratio as low as 2.2%.					
31464438	4	25	dep	species	558:564	arg1	duck					588:591	duck	588:591	duck	588:591	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	25	dep	species	558:564	arg1	mutton					598:603	mutton	598:603	mutton	598:603	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	25	dep	species	558:564	arg1	pork					582:585	pork	582:585	pork	582:585	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	25	dep	species	558:564	arg1	chicken					573:579	chicken	573:579	chicken	573:579	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	4	25	dep	species	558:564	arg1	beef					567:570	beef	567:570	beef	567:570	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	3	26	theme	N-glycosylation	389:403	arg1	species					437:443	species	437:443	species	437:443	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	26	theme	N-glycosylation	389:403	arg1	profiles					405:412	the protein N-glycosylation profiles	377:412	the protein N-glycosylation profiles of different meats	377:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	26	theme	N-glycosylation	389:403	arg1	specific					445:452	specific	445:452	specific	445:452	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	6	27	theme	distinguishing	939:952	arg1	N-glycans					954:962	The most distinguishing N-glycans	930:962	The most distinguishing N-glycans from beef and duck	930:981	The most distinguishing N-glycans from beef and duck were elucidated for the detailed structures.					
31464438	3	28	used	used	470:473	arg2	profiles					405:412	the protein N-glycosylation profiles	377:412	the protein N-glycosylation profiles of different meats	377:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	28	used	used	470:473	arg2	species					437:443	species	437:443	species	437:443	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	28	used	used	470:473	arg2	specific					445:452	specific	445:452	specific	445:452	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	1	29	theme	meat	106:109	arg1	Adulteration					90:101	Adulteration	90:101	Adulteration of meat and meat products	90:127	Adulteration of meat and meat products causes concerns to consumers.					
31464438	0	30	theme	Qualitative	30:40	arg1	Analysis					59:66	Qualitative and Quantitative Analysis	30:66	Qualitative and Quantitative Analysis of Meat Adulteration	30:87	N-Glycan Profile as a Tool in Qualitative and Quantitative Analysis of Meat Adulteration.					
31464438	4	31	theme	N-glycan	513:520	arg1	pattern					522:528	the N-glycan pattern	509:528	the N-glycan pattern	509:528	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	2	32	theme	robust	192:197	arg1	methods					213:219	novel robust and sensitive methods	186:219	novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods	186:348	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	4	33	theme	component	640:648	arg1	analysis					650:657	principal component analysis	630:657	principal component analysis	630:657	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	2	34	theme	novel	186:190	arg1	methods					213:219	novel robust and sensitive methods	186:219	novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods	186:348	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31464438	4	35	theme	calibration	721:731	arg1	model					748:752	a calibration and validation model	719:752	a calibration and validation model for the prediction of adulteration ratio	719:793	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	1	36	theme	meat	115:118	arg1	Adulteration					90:101	Adulteration	90:101	Adulteration of meat and meat products	90:127	Adulteration of meat and meat products causes concerns to consumers.					
31464438	4	37	dep	partial	664:670	arg1	square					678:683	square	678:683	square	678:683	Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio.					
31464438	5	38	theme	duck	859:862	arg1	meat					864:867	a lower-value duck meat	845:867	a lower-value duck meat	845:867	Using this method, beef samples adulterated with a lower-value duck meat could be detected down to the addition ratio as low as 2.2%.					
31464438	5	39	theme	addition	899:906	arg1	ratio					908:912	the addition ratio	895:912	the addition ratio	895:912	Using this method, beef samples adulterated with a lower-value duck meat could be detected down to the addition ratio as low as 2.2%.					
31464438	3	40	dep	specific	445:452	arg1	species					437:443	species	437:443	species	437:443	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	40	dep	specific	445:452	arg1	profiles					405:412	the protein N-glycosylation profiles	377:412	the protein N-glycosylation profiles of different meats	377:431	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	3	40	dep	specific	445:452	arg1	specific					445:452	specific	445:452	specific	445:452	This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication.					
31464438	5	41	theme	beef	815:818	arg1	samples					820:826	beef samples	815:826	beef samples adulterated with a lower-value duck meat	815:867	Using this method, beef samples adulterated with a lower-value duck meat could be detected down to the addition ratio as low as 2.2%.					
31464438	0	42	theme	Quantitative	46:57	arg1	Analysis					59:66	Qualitative and Quantitative Analysis	30:66	Qualitative and Quantitative Analysis of Meat Adulteration	30:87	N-Glycan Profile as a Tool in Qualitative and Quantitative Analysis of Meat Adulteration.					
31464438	2	43	theme	methods	342:348	arg1	drawbacks					316:324	the drawbacks	312:324	the drawbacks of the existing methods	312:348	It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods.					
31747749	4	0	theme	profile	462:468	arg1	variation					433:441	phenotypic variation	422:441	phenotypic variation of plasma N-glycan profile	422:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	6	1	from	contribution	723:734	arg1	variation					775:783	plasma N-glycome variation	758:783	plasma N-glycome variation	758:783	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	7	2	theme	higher	888:893	arg1	contribution					909:920	higher environmental contribution	888:920	higher environmental contribution to their variation	888:939	We have also found several structures with higher environmental contribution to their variation.					
31747749	5	3	theme	data	606:609	arg1	set					611:613	the TwinsUK data set	594:613	the TwinsUK data set	594:613	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	4	4	theme	N-glycan	453:460	arg1	profile					462:468	plasma N-glycan profile	446:468	plasma N-glycan profile	446:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	7	5	theme	several	864:870	arg1	structures					872:881	several structures	864:881	several structures	864:881	We have also found several structures with higher environmental contribution to their variation.					
31747749	6	6	from	variation	775:783	arg1	contribution					723:734	a significant contribution	709:734	a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins	709:842	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	4	7	theme	genetic	403:409	arg1	factors					411:417	genetic factors	403:417	genetic factors	403:417	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	3	8	theme	relative	253:260	arg1	importance					262:271	relative importance	253:271	relative importance of genetic and environmental factors in regulating plasma N-glycome	253:339	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	1	9	theme	total	71:75	arg1	proteins					90:97	total human plasma proteins	71:97	total human plasma proteins	71:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	3	10	theme	importance	262:271	arg1	scarce					345:350	scarce	345:350	scarce	345:350	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	3	10	theme	importance	262:271	arg1	studies					242:248	studies	242:248	studies of relative importance of genetic and environmental factors in regulating plasma N-glycome	242:339	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	1	11	theme	human	77:81	arg1	proteins					90:97	total human plasma proteins	71:97	total human plasma proteins	71:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	4	12	theme	study	368:372	arg1	aim					357:359	The aim	353:359	The aim of our study	353:372	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	1	13	theme	plasma	83:88	arg1	proteins					90:97	total human plasma proteins	71:97	total human plasma proteins	71:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	5	14	theme	Thirty-nine	513:523	arg1	traits					548:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	4	15	theme	phenotypic	422:431	arg1	variation					433:441	phenotypic variation	422:441	phenotypic variation of plasma N-glycan profile	422:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	6	16	theme	N-glycome	765:773	arg1	variation					775:783	plasma N-glycome variation	758:783	plasma N-glycome variation	758:783	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	1	17	theme	proteins	90:97	arg1	biomarker					117:125	a useful biomarker	108:125	a useful biomarker for various pathological states	108:157	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	1	17	theme	proteins	90:97	arg1	profile					60:66	The N-glycosylation profile	40:66	The N-glycosylation profile of total human plasma proteins	40:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	0	18	theme	Plasma	22:27	arg1	N-Glycome					29:37	Human Plasma N-Glycome	16:37	Human Plasma N-Glycome	16:37	Heritability of Human Plasma N-Glycome.					
31747749	5	19	theme	TwinsUK	598:604	arg1	set					611:613	the TwinsUK data set	594:613	the TwinsUK data set	594:613	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	0	20	theme	Human	16:20	arg1	N-Glycome					29:37	Human Plasma N-Glycome	16:37	Human Plasma N-Glycome	16:37	Heritability of Human Plasma N-Glycome.					
31747749	3	21	theme	environmental	288:300	arg1	factors					302:308	genetic and environmental factors	276:308	genetic and environmental factors	276:308	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	4	22	theme	plasma	446:451	arg1	profile					462:468	plasma N-glycan profile	446:468	plasma N-glycan profile	446:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	5	23	from	set	611:613	arg1	individuals					577:587	2816 individuals	572:587	2816 individuals from the TwinsUK data set	572:613	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	0	24	theme	N-Glycome	29:37	arg1	Heritability					0:11	Heritability	0:11	Heritability of Human Plasma N-Glycome	0:37	Heritability of Human Plasma N-Glycome.					
31747749	3	25	theme	genetic	276:282	arg1	factors					302:308	genetic and environmental factors	276:308	genetic and environmental factors	276:308	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	2	26	theme	high-throughput	169:183	arg1	methods					185:191	Reliable high-throughput methods	160:191	Reliable high-throughput methods for such profiling	160:210	Reliable high-throughput methods for such profiling have been developed.					
31747749	6	27	theme	significant	711:721	arg1	contribution					723:734	a significant contribution	709:734	a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins	709:842	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	1	28	gly	N-glycosylation	44:58	arg1	proteins					90:97	total human plasma proteins	71:97	total human plasma proteins	71:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	2	29	theme	Reliable	160:167	arg1	methods					185:191	Reliable high-throughput methods	160:191	Reliable high-throughput methods for such profiling	160:210	Reliable high-throughput methods for such profiling have been developed.					
31747749	6	30	theme	factors	747:753	arg1	contribution					723:734	a significant contribution	709:734	a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins	709:842	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	3	31	theme	plasma	324:329	arg1	N-glycome					331:339	plasma N-glycome	324:339	plasma N-glycome	324:339	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	6	32	theme	genetic	739:745	arg1	factors					747:753	genetic factors	739:753	genetic factors	739:753	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	1	33	theme	useful	110:115	arg1	biomarker					117:125	a useful biomarker	108:125	a useful biomarker for various pathological states	108:157	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	1	33	theme	useful	110:115	arg1	profile					60:66	The N-glycosylation profile	40:66	The N-glycosylation profile of total human plasma proteins	40:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	6	34	theme	heritability	653:664	arg1	estimates					666:674	high heritability estimates	648:674	high heritability estimates (>50%)	648:681	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	6	34	theme	heritability	653:664	arg1	%					680:680	>50%	677:680	>50%	677:680	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	6	35	theme	plasma	758:763	arg1	variation					775:783	plasma N-glycome variation	758:783	plasma N-glycome variation	758:783	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	3	36	theme	factors	302:308	arg1	importance					262:271	relative importance	253:271	relative importance of genetic and environmental factors in regulating plasma N-glycome	253:339	However, studies of relative importance of genetic and environmental factors in regulating plasma N-glycome are scarce.					
31747749	4	37	theme	factors	411:417	arg1	role					395:398	the role	391:398	the role of genetic factors in phenotypic variation of plasma N-glycan profile	391:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	6	38	attach	attached	816:823	arg1	immunoglobulins					828:842	immunoglobulins	828:842	immunoglobulins	828:842	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	6	38	attach	attached	816:823	arg2	glycans					801:807	glycans	801:807	glycans mostly attached to immunoglobulins	801:842	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	6	39	theme	high	648:651	arg1	estimates					666:674	high heritability estimates	648:674	high heritability estimates (>50%)	648:681	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	6	39	theme	high	648:651	arg1	%					680:680	>50%	677:680	>50%	677:680	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	5	40	theme	total	525:529	arg1	traits					548:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	6	41	from	factors	747:753	arg1	variation					775:783	plasma N-glycome variation	758:783	plasma N-glycome variation	758:783	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	7	42	theme	environmental	895:907	arg1	contribution					909:920	higher environmental contribution	888:920	higher environmental contribution to their variation	888:939	We have also found several structures with higher environmental contribution to their variation.					
31747749	2	43	theme	such	197:200	arg1	profiling					202:210	such profiling	197:210	such profiling	197:210	Reliable high-throughput methods for such profiling have been developed.					
31747749	5	44	theme	plasma	531:536	arg1	traits					548:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	1	45	theme	various	131:137	arg1	states					152:157	various pathological states	131:157	various pathological states	131:157	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	6	46	theme	traits	640:645	arg1	majority					624:631	the majority	620:631	the majority of the traits	620:645	For the majority of the traits, high heritability estimates (>50%) were obtained pointing at a significant contribution of genetic factors in plasma N-glycome variation, especially for glycans mostly attached to immunoglobulins.					
31747749	4	47	from	role	395:398	arg1	variation					433:441	phenotypic variation	422:441	phenotypic variation of plasma N-glycan profile	422:468	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	4	48	theme	heritability	499:510	arg1	estimates					482:490	the estimates	478:490	the estimates of its heritability	478:510	The aim of our study was to determine the role of genetic factors in phenotypic variation of plasma N-glycan profile through the estimates of its heritability.					
31747749	1	49	theme	N-glycosylation	44:58	arg1	biomarker					117:125	a useful biomarker	108:125	a useful biomarker for various pathological states	108:157	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	1	49	theme	N-glycosylation	44:58	arg1	profile					60:66	The N-glycosylation profile	40:66	The N-glycosylation profile of total human plasma proteins	40:97	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31747749	5	50	theme	N-glycome	538:546	arg1	traits					548:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits	513:553	Thirty-nine total plasma N-glycome traits were analyzed in 2816 individuals from the TwinsUK data set.					
31747749	1	51	theme	pathological	139:150	arg1	states					152:157	various pathological states	131:157	various pathological states	131:157	The N-glycosylation profile of total human plasma proteins could be a useful biomarker for various pathological states.					
31256376	6	0	link	N-linked	784:791	arg1	carbohydrates					793:805	the common N-linked carbohydrates	773:805	the common N-linked carbohydrates unaltered from proteins	773:829	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	1	theme	N-linked	784:791	arg1	carbohydrates					793:805	the common N-linked carbohydrates	773:805	the common N-linked carbohydrates unaltered from proteins	773:829	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	10	2	link	O-linked	1351:1358	arg1	carbohydrates					1360:1372	all the different O-linked carbohydrates	1333:1372	all the different O-linked carbohydrates	1333:1372	Chemical methods are used to liberate these oligosaccharides because no enzyme would remove all the different O-linked carbohydrates.					
31256376	5	3	theme	core	666:669	arg1	structure					671:679	a common core structure	657:679	a common core structure of GlcNAc2Man3	657:694	N-linked oligosaccharides share a common core structure of GlcNAc2Man3.					
31256376	4	4	gly	N-glycosylated	492:505	arg1	species					507:513	N-glycosylated species	492:513	N-glycosylated species	492:513	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	15	5	gly	glycopeptides	2213:2225	arg2	glycopeptides					2213:2225	glycopeptides	2213:2225	glycopeptides	2213:2225	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	16	6	theme	MS/MS	2268:2272	arg1	electron-capture					2286:2301	electron-capture	2286:2301	electron-capture	2286:2301	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	6	theme	MS/MS	2268:2272	arg1	dissociation					2325:2336	electron-transfer dissociation	2307:2336	electron-transfer dissociation	2307:2336	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	6	theme	MS/MS	2268:2272	arg1	techniques					2274:2283	ESI-compatible alternative MS/MS techniques	2241:2283	ESI-compatible alternative MS/MS techniques	2241:2283	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	9	7	theme	elongated	1187:1195	arg1	structures					1197:1206	corresponding elongated structures	1173:1206	corresponding elongated structures	1173:1206	Mammalian proteins have been reported bearing O-linked N-acetylgalactosamine, fucose, glucose, xylose, mannose, and corresponding elongated structures, as well as N-acetylglucosamine.					
31256376	15	8	theme	glycopeptides	2213:2225	arg1	detection					2200:2208	the selective and specific detection	2173:2208	the selective and specific detection of glycopeptides	2173:2225	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	16	9	theme	glycopeptide	2343:2354	arg1	identification					2356:2369	glycopeptide identification	2343:2369	glycopeptide identification as well as modification site assignments	2343:2410	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	6	10	theme	glycosylated	864:875	arg1	residue					881:887	the originally glycosylated Asn residue	849:887	the originally glycosylated Asn residue to Asp	849:894	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	2	11	theme	acid	311:314	arg1	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	11	12	theme	carbohydrate	1549:1560	arg1	structures					1562:1571	different carbohydrate structures	1539:1571	different carbohydrate structures	1539:1571	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	15	13	theme	mass	1952:1955	arg1	spectrometer					1957:1968	a mass spectrometer	1950:1968	a mass spectrometer	1950:1968	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	2	14	theme	certain	297:303	arg1	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	13	15	theme	mass	1768:1771	arg1	spectrometry					1773:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	For addressing site-specific carbohydrate heterogeneity mass spectrometry has become the method of choice.					
31256376	14	16	theme	best	1929:1932	arg1	solution					1934:1941	the best solution	1925:1941	the best solution	1925:1941	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	13	17	theme	choice	1811:1816	arg1	method					1801:1806	the method	1797:1806	the method of choice	1797:1816	For addressing site-specific carbohydrate heterogeneity mass spectrometry has become the method of choice.					
31256376	16	18	gly	glycopeptide	2343:2354	arg2	glycopeptide					2343:2354	glycopeptide identification	2343:2369	glycopeptide identification as well as modification site assignments	2343:2410	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	12	19	theme	site-specific	1601:1613	arg1	heterogeneity					1615:1627	This site-specific heterogeneity	1596:1627	This site-specific heterogeneity	1596:1627	This site-specific heterogeneity may vary by species and tissue, and may also be affected by physiological changes.					
31256376	7	20	gly	O-glycosylation	897:911	arg2	residues					941:948	Tyr residues	937:948	Tyr residues	937:948	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	7	20	gly	O-glycosylation	897:911	arg2	Thr					928:930	Thr	928:930	Thr	928:930	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	7	20	gly	O-glycosylation	897:911	arg2	stretches					971:979	sequence stretches	962:979	sequence stretches rich in hydroxy-amino acids	962:1007	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	7	20	gly	O-glycosylation	897:911	arg2	Ser					923:925	Ser	923:925	Ser	923:925	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	11	21	theme	structures	1562:1571	arg1	array					1530:1534	an array	1527:1534	an array of different carbohydrate structures	1527:1571	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	11	22	theme	molecules	1505:1513	arg1	population					1483:1492	a population	1481:1492	a population of protein molecules	1481:1513	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	10	23	used	used	1262:1265	arg2	methods					1250:1256	Chemical methods	1241:1256	Chemical methods	1241:1256	Chemical methods are used to liberate these oligosaccharides because no enzyme would remove all the different O-linked carbohydrates.					
31256376	12	24	theme	physiological	1689:1701	arg1	changes					1703:1709	physiological changes	1689:1709	physiological changes	1689:1709	This site-specific heterogeneity may vary by species and tissue, and may also be affected by physiological changes.					
31256376	16	25	theme	ESI-compatible	2241:2254	arg1	electron-capture					2286:2301	electron-capture	2286:2301	electron-capture	2286:2301	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	25	theme	ESI-compatible	2241:2254	arg1	dissociation					2325:2336	electron-transfer dissociation	2307:2336	electron-transfer dissociation	2307:2336	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	25	theme	ESI-compatible	2241:2254	arg1	techniques					2274:2283	ESI-compatible alternative MS/MS techniques	2241:2283	ESI-compatible alternative MS/MS techniques	2241:2283	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	4	26	theme	N-glycosylated	492:505	arg1	species					507:513	N-glycosylated species	492:513	N-glycosylated species	492:513	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	16	27	theme	modification	2382:2393	arg1	assignments					2400:2410	modification site assignments	2382:2410	glycopeptide identification as well as modification site assignments	2343:2410	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	7	28	theme	Tyr	937:939	arg1	residues					941:948	Tyr residues	937:948	Tyr residues	937:948	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	1	29	theme	sequence	161:168	arg1	motif					170:174	such a sequence motif	154:174	such a sequence motif	154:174	Even if a consensus sequence has been identified for a posttranslational modification, the presence of such a sequence motif only indicates the possibility, not the certainty that the modification actually occurs.					
31256376	13	30	theme	site-specific	1727:1739	arg1	spectrometry					1773:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	For addressing site-specific carbohydrate heterogeneity mass spectrometry has become the method of choice.					
31256376	10	31	theme	Chemical	1241:1248	arg1	methods					1250:1256	Chemical methods	1241:1256	Chemical methods	1241:1256	Chemical methods are used to liberate these oligosaccharides because no enzyme would remove all the different O-linked carbohydrates.					
31256376	15	32	theme	carbohydrate	2099:2110	arg1	ions					2112:2115	diagnostic carbohydrate ions	2088:2115	the same time diagnostic carbohydrate ions	2074:2115	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	9	33	theme	Mammalian	1057:1065	arg1	proteins					1067:1074	Mammalian proteins	1057:1074	Mammalian proteins	1057:1074	Mammalian proteins have been reported bearing O-linked N-acetylgalactosamine, fucose, glucose, xylose, mannose, and corresponding elongated structures, as well as N-acetylglucosamine.					
31256376	5	34	link	N-linked	625:632	arg1	oligosaccharides					634:649	N-linked oligosaccharides	625:649	N-linked oligosaccharides	625:649	N-linked oligosaccharides share a common core structure of GlcNAc2Man3.					
31256376	15	35	theme	online	1973:1978	arg1	detector					1980:1987	online detector	1973:1987	online detector	1973:1987	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	14	36	theme	electrospray	1861:1872	arg1	spectrometry					1890:1901	electrospray ionization mass spectrometry	1861:1901	electrospray ionization mass spectrometry (LC/ESI-MS/MS)	1861:1916	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	14	36	theme	electrospray	1861:1872	arg1	LC/ESI-MS/MS					1904:1915	LC/ESI-MS/MS	1904:1915	LC/ESI-MS/MS	1904:1915	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	8	37	theme	core	1041:1044	arg1	structure					1046:1054	a common core structure	1032:1054	a common core structure	1032:1054	O-glycosylation lacks a common core structure.					
31256376	4	38	theme	Asn-Xxx-Ser/Thr/Cys	547:565	arg1	sequons					567:573	Asn-Xxx-Ser/Thr/Cys sequons	547:573	Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline)	547:622	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	11	39	theme	different	1539:1547	arg1	structures					1562:1571	different carbohydrate structures	1539:1571	different carbohydrate structures	1539:1571	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	0	40	theme	Site-Specific	20:32	arg1	N-Glycosylation					34:48	Site-Specific N-Glycosylation	20:48	Site-Specific N-Glycosylation	20:48	Characterization of Site-Specific N-Glycosylation.					
31256376	1	41	theme	posttranslational	106:122	arg1	modification					124:135	a posttranslational modification	104:135	a posttranslational modification	104:135	Even if a consensus sequence has been identified for a posttranslational modification, the presence of such a sequence motif only indicates the possibility, not the certainty that the modification actually occurs.					
31256376	15	42	theme	mass	2047:2050	arg1	mapping					2052:2058	every component eluting (mass mapping)	2022:2059	every component eluting (mass mapping)	2022:2059	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	9	43	theme	O-linked	1103:1110	arg1	N-acetylgalactosamine					1112:1132	O-linked N-acetylgalactosamine	1103:1132	O-linked N-acetylgalactosamine	1103:1132	Mammalian proteins have been reported bearing O-linked N-acetylgalactosamine, fucose, glucose, xylose, mannose, and corresponding elongated structures, as well as N-acetylglucosamine.					
31256376	15	44	theme	eluting	2038:2044	arg1	mapping					2052:2058	every component eluting (mass mapping)	2022:2059	every component eluting (mass mapping)	2022:2059	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	6	45	theme	PNGase	746:751	arg1	F					743:743	peptide N-glycosidase F	721:743	peptide N-glycosidase F (PNGase F)	721:754	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	45	theme	PNGase	746:751	arg1	F					753:753	PNGase F	746:753	PNGase F	746:753	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	15	46	theme	collisional	2137:2147	arg1	activation					2149:2158	collisional activation	2137:2158	collisional activation that permits the selective and specific detection of glycopeptides	2137:2225	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	15	47	dep	time	2083:2086	arg1	ions					2112:2115	diagnostic carbohydrate ions	2088:2115	the same time diagnostic carbohydrate ions	2074:2115	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	3	48	theme	defined	467:473	arg1	motif					485:489	a relatively loosely defined consensus motif	446:489	a relatively loosely defined consensus motif	446:489	C-mannosylation occurs on Trp residues within a relatively loosely defined consensus motif.					
31256376	4	49	theme	amino	597:601	arg1	acid					603:606	any amino acid	593:606	any amino acid	593:606	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	11	50	theme	N-	1400:1401	arg1	Characterization					1375:1390	Characterization	1375:1390	Characterization of both N- and O-glycosylation	1375:1421	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	6	51	theme	peptide	721:727	arg1	enzyme					713:718	an enzyme	710:718	an enzyme	710:718	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	51	theme	peptide	721:727	arg1	F					743:743	peptide N-glycosidase F	721:743	peptide N-glycosidase F (PNGase F)	721:754	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	51	theme	peptide	721:727	arg1	F					753:753	PNGase F	746:753	PNGase F	746:753	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	16	52	theme	alternative	2256:2266	arg1	electron-capture					2286:2301	electron-capture	2286:2301	electron-capture	2286:2301	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	52	theme	alternative	2256:2266	arg1	dissociation					2325:2336	electron-transfer dissociation	2307:2336	electron-transfer dissociation	2307:2336	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	52	theme	alternative	2256:2266	arg1	techniques					2274:2283	ESI-compatible alternative MS/MS techniques	2241:2283	ESI-compatible alternative MS/MS techniques	2241:2283	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	10	53	theme	O-linked	1351:1358	arg1	carbohydrates					1360:1372	all the different O-linked carbohydrates	1333:1372	all the different O-linked carbohydrates	1333:1372	Chemical methods are used to liberate these oligosaccharides because no enzyme would remove all the different O-linked carbohydrates.					
31256376	9	54	link	O-linked	1103:1110	arg1	N-acetylgalactosamine					1112:1132	O-linked N-acetylgalactosamine	1103:1132	O-linked N-acetylgalactosamine	1103:1132	Mammalian proteins have been reported bearing O-linked N-acetylgalactosamine, fucose, glucose, xylose, mannose, and corresponding elongated structures, as well as N-acetylglucosamine.					
31256376	2	55	gly	glycosylated	281:292	arg2	Proteins					265:272	Proteins	265:272	Proteins	265:272	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	2	55	gly	glycosylated	281:292	arg2	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	2	55	gly	glycosylated	281:292	arg1	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	2	55	gly	glycosylated	281:292	arg1	Proteins					265:272	Proteins	265:272	Proteins	265:272	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	5	56	theme	common	659:664	arg1	structure					671:679	a common core structure	657:679	a common core structure of GlcNAc2Man3	657:694	N-linked oligosaccharides share a common core structure of GlcNAc2Man3.					
31256376	6	57	theme	common	777:782	arg1	carbohydrates					793:805	the common N-linked carbohydrates	773:805	the common N-linked carbohydrates unaltered from proteins	773:829	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	11	58	theme	O-glycosylation	1407:1421	arg1	Characterization					1375:1390	Characterization	1375:1390	Characterization of both N- and O-glycosylation	1375:1421	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	9	59	theme	corresponding	1173:1185	arg1	structures					1197:1206	corresponding elongated structures	1173:1206	corresponding elongated structures	1173:1206	Mammalian proteins have been reported bearing O-linked N-acetylgalactosamine, fucose, glucose, xylose, mannose, and corresponding elongated structures, as well as N-acetylglucosamine.					
31256376	1	60	theme	motif	170:174	arg1	presence					142:149	the presence	138:149	the presence of such a sequence motif	138:174	Even if a consensus sequence has been identified for a posttranslational modification, the presence of such a sequence motif only indicates the possibility, not the certainty that the modification actually occurs.					
31256376	6	61	theme	Asn	877:879	arg1	residue					881:887	the originally glycosylated Asn residue	849:887	the originally glycosylated Asn residue to Asp	849:894	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	5	62	theme	N-linked	625:632	arg1	oligosaccharides					634:649	N-linked oligosaccharides	625:649	N-linked oligosaccharides	625:649	N-linked oligosaccharides share a common core structure of GlcNAc2Man3.					
31256376	2	63	theme	side	316:319	arg1	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	15	64	theme	specific	2191:2198	arg1	detection					2200:2208	the selective and specific detection	2173:2208	the selective and specific detection of glycopeptides	2173:2225	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	2	65	theme	amino	305:309	arg1	chains					321:326	certain amino acid side chains	297:326	certain amino acid side chains	297:326	Proteins can be glycosylated on certain amino acid side chains, and these modifications are designated as C-, N-, and O-glycosylation.					
31256376	15	66	theme	selective	2177:2185	arg1	detection					2200:2208	the selective and specific detection	2173:2208	the selective and specific detection of glycopeptides	2173:2225	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	14	67	theme	ionization	1874:1883	arg1	spectrometry					1890:1901	electrospray ionization mass spectrometry	1861:1901	electrospray ionization mass spectrometry (LC/ESI-MS/MS)	1861:1916	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	14	67	theme	ionization	1874:1883	arg1	LC/ESI-MS/MS					1904:1915	LC/ESI-MS/MS	1904:1915	LC/ESI-MS/MS	1904:1915	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	7	68	from	rich	981:984	arg1	acids					1003:1007	hydroxy-amino acids	989:1007	hydroxy-amino acids	989:1007	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	13	69	theme	heterogeneity	1754:1766	arg1	spectrometry					1773:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	For addressing site-specific carbohydrate heterogeneity mass spectrometry has become the method of choice.					
31256376	6	70	from	proteins	822:829	arg1	carbohydrates					793:805	the common N-linked carbohydrates	773:805	the common N-linked carbohydrates unaltered from proteins	773:829	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	7	71	theme	hydroxy-amino	989:1001	arg1	acids					1003:1007	hydroxy-amino acids	989:1007	hydroxy-amino acids	989:1007	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	11	72	theme	protein	1497:1503	arg1	molecules					1505:1513	protein molecules	1497:1513	protein molecules	1497:1513	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	5	73	theme	GlcNAc2Man3	684:694	arg1	structure					671:679	a common core structure	657:679	a common core structure of GlcNAc2Man3	657:694	N-linked oligosaccharides share a common core structure of GlcNAc2Man3.					
31256376	7	74	theme	rich	981:984	arg1	stretches					971:979	sequence stretches	962:979	sequence stretches rich in hydroxy-amino acids	962:1007	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	15	75	theme	diagnostic	2088:2097	arg1	ions					2112:2115	diagnostic carbohydrate ions	2088:2115	the same time diagnostic carbohydrate ions	2074:2115	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	6	76	gly	glycosylated	864:875	arg1	residue					881:887	the originally glycosylated Asn residue	849:887	the originally glycosylated Asn residue to Asp	849:894	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	7	77	theme	sequence	962:969	arg1	stretches					971:979	sequence stretches	962:979	sequence stretches rich in hydroxy-amino acids	962:1007	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	16	78	theme	site	2395:2398	arg1	assignments					2400:2410	modification site assignments	2382:2410	glycopeptide identification as well as modification site assignments	2343:2410	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	3	79	gly	C-mannosylation	400:414	arg2	residues					430:437	Trp residues	426:437	Trp residues within a relatively loosely defined consensus motif	426:489	C-mannosylation occurs on Trp residues within a relatively loosely defined consensus motif.					
31256376	14	80	theme	mass	1885:1888	arg1	spectrometry					1890:1901	electrospray ionization mass spectrometry	1861:1901	electrospray ionization mass spectrometry (LC/ESI-MS/MS)	1861:1916	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	14	80	theme	mass	1885:1888	arg1	LC/ESI-MS/MS					1904:1915	LC/ESI-MS/MS	1904:1915	LC/ESI-MS/MS	1904:1915	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	1	81	theme	consensus	61:69	arg1	sequence					71:78	a consensus sequence	59:78	a consensus sequence	59:78	Even if a consensus sequence has been identified for a posttranslational modification, the presence of such a sequence motif only indicates the possibility, not the certainty that the modification actually occurs.					
31256376	13	82	theme	carbohydrate	1741:1752	arg1	spectrometry					1773:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	site-specific carbohydrate heterogeneity mass spectrometry	1727:1784	For addressing site-specific carbohydrate heterogeneity mass spectrometry has become the method of choice.					
31256376	8	83	theme	common	1034:1039	arg1	structure					1046:1054	a common core structure	1032:1054	a common core structure	1032:1054	O-glycosylation lacks a common core structure.					
31256376	15	84	theme	same	2078:2081	arg1	time					2083:2086	the same time diagnostic carbohydrate ions	2074:2115	the same time diagnostic carbohydrate ions	2074:2115	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	16	85	theme	electron-transfer	2307:2323	arg1	dissociation					2325:2336	electron-transfer dissociation	2307:2336	electron-transfer dissociation	2307:2336	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	16	85	theme	electron-transfer	2307:2323	arg1	techniques					2274:2283	ESI-compatible alternative MS/MS techniques	2241:2283	ESI-compatible alternative MS/MS techniques	2241:2283	In addition, ESI-compatible alternative MS/MS techniques, electron-capture and electron-transfer dissociation, aid glycopeptide identification as well as modification site assignments.					
31256376	4	86	theme	sequons	567:573	arg1	residues					535:542	Asn residues	531:542	Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline)	531:622	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	4	86	theme	sequons	567:573	arg1	sequons					567:573	Asn-Xxx-Ser/Thr/Cys sequons	547:573	Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline)	547:622	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	0	87	theme	N-Glycosylation	34:48	arg1	Characterization					0:15	Characterization	0:15	Characterization of Site-Specific N-Glycosylation.	0:49	Characterization of Site-Specific N-Glycosylation.					
31256376	11	88	theme	same	1459:1462	arg1	positions					1464:1472	the same positions	1455:1472	the same positions within a population of protein molecules	1455:1513	Characterization of both N- and O-glycosylation is complicated by the fact that the same positions within a population of protein molecules may feature an array of different carbohydrate structures, or remain unmodified.					
31256376	3	89	theme	Trp	426:428	arg1	residues					430:437	Trp residues	426:437	Trp residues within a relatively loosely defined consensus motif	426:489	C-mannosylation occurs on Trp residues within a relatively loosely defined consensus motif.					
31256376	4	90	theme	Asn	531:533	arg1	residues					535:542	Asn residues	531:542	Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline)	531:622	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	4	90	theme	Asn	531:533	arg1	sequons					567:573	Asn-Xxx-Ser/Thr/Cys sequons	547:573	Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline)	547:622	N-glycosylated species are modified at Asn residues of Asn-Xxx-Ser/Thr/Cys sequons (where Xxx can be any amino acid except proline).					
31256376	15	91	theme	mapping	2052:2058	arg1	analysis					2010:2017	the analysis	2006:2017	the analysis of every component eluting (mass mapping)	2006:2059	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	10	92	theme	different	1341:1349	arg1	carbohydrates					1360:1372	all the different O-linked carbohydrates	1333:1372	all the different O-linked carbohydrates	1333:1372	Chemical methods are used to liberate these oligosaccharides because no enzyme would remove all the different O-linked carbohydrates.					
31256376	14	93	theme	Reversed-phase	1819:1832	arg1	HPLC					1834:1837	Reversed-phase HPLC	1819:1837	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS)	1819:1916	Reversed-phase HPLC directly coupled with electrospray ionization mass spectrometry (LC/ESI-MS/MS) offers the best solution.					
31256376	7	94	from	acids	1003:1007	arg1	rich					981:984	rich	981:984	rich	981:984	O-glycosylation occurs at Ser, Thr, and Tyr residues, usually in sequence stretches rich in hydroxy-amino acids.					
31256376	15	95	theme	component	2028:2036	arg1	mapping					2052:2058	every component eluting (mass mapping)	2022:2059	every component eluting (mass mapping)	2022:2059	Using a mass spectrometer as online detector not only assures the analysis of every component eluting (mass mapping), but also at the same time diagnostic carbohydrate ions can be generated by collisional activation that permits the selective and specific detection of glycopeptides.					
31256376	6	96	theme	N-glycosidase	729:741	arg1	enzyme					713:718	an enzyme	710:718	an enzyme	710:718	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	96	theme	N-glycosidase	729:741	arg1	F					743:743	peptide N-glycosidase F	721:743	peptide N-glycosidase F (PNGase F)	721:754	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	6	96	theme	N-glycosidase	729:741	arg1	F					753:753	PNGase F	746:753	PNGase F	746:753	In addition, an enzyme, peptide N-glycosidase F (PNGase F), removes most of the common N-linked carbohydrates unaltered from proteins while hydrolyzing the originally glycosylated Asn residue to Asp.					
31256376	3	97	theme	consensus	475:483	arg1	motif					485:489	a relatively loosely defined consensus motif	446:489	a relatively loosely defined consensus motif	446:489	C-mannosylation occurs on Trp residues within a relatively loosely defined consensus motif.					
31349756	2	0	theme	glycans	549:555	arg1	occupation					497:506	site-specific occupation	483:506	site-specific occupation	483:506	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	2	0	theme	glycans	549:555	arg1	diversity					523:531	structural diversity	512:531	structural diversity	512:531	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	2	0	theme	glycans	549:555	arg1	localization					469:480	glycosylation-site localization	450:480	glycosylation-site localization	450:480	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	7	1	theme	protein	1251:1257	arg1	species					1259:1265	the de-N-glycosylated intact protein species	1222:1265	the de-N-glycosylated intact protein species	1222:1265	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	1	2	theme	autoimmune	172:181	arg1	diseases					183:190	autoimmune diseases	172:190	autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	172:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	5	3	theme	site	848:851	arg1	microheterogeneity					853:870	the N-glycosylation site microheterogeneity	828:870	the N-glycosylation site microheterogeneity	828:870	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	6	4	theme	region	1110:1115	arg1	sites					1133:1137	two hinge region O-glycosylation sites	1100:1137	two hinge region O-glycosylation sites with core 1-type glycans	1100:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	2	5	theme	attached	540:547	arg1	glycans					549:555	the attached glycans	536:555	the attached glycans	536:555	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	6	6	theme	site	1027:1030	arg1	presence					995:1002	the presence	991:1002	the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans	991:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	7	7	gly	de-N-glycosylated	1226:1242	arg1	species					1259:1265	the de-N-glycosylated intact protein species	1222:1265	the de-N-glycosylated intact protein species	1222:1265	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	7	8	theme	intact	1244:1249	arg1	species					1259:1265	the de-N-glycosylated intact protein species	1222:1265	the de-N-glycosylated intact protein species	1222:1265	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	6	9	gly	N-glycosylation	1011:1025	arg2	site					1027:1030	one N-glycosylation site	1007:1030	one N-glycosylation site	1007:1030	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	9	gly	N-glycosylation	1011:1025	arg2	one					1007:1009	one	1007:1009	one	1007:1009	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	10	theme	hinge	1104:1108	arg1	region					1110:1115	hinge region	1104:1115	two hinge region O-glycosylation sites with core 1-type glycans	1100:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	11	theme	N-glycosylation	1011:1025	arg1	site					1027:1030	one N-glycosylation site	1007:1030	one N-glycosylation site	1007:1030	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	7	12	theme	de-N-glycosylated	1226:1242	arg1	species					1259:1265	the de-N-glycosylated intact protein species	1222:1265	the de-N-glycosylated intact protein species	1222:1265	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	3	13	theme	site-specific	597:609	arg1	N-					611:612	comprehensive site-specific N-	583:612	comprehensive site-specific N-	583:612	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	3	14	theme	O-glycosylation	618:632	arg1	characterization					634:649	a first comprehensive site-specific N- and O-glycosylation characterization	575:649	a first comprehensive site-specific N- and O-glycosylation characterization of atacicept	575:662	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	3	15	theme	N-	611:612	arg1	characterization					634:649	a first comprehensive site-specific N- and O-glycosylation characterization	575:649	a first comprehensive site-specific N- and O-glycosylation characterization of atacicept	575:662	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	1	16	from	application	157:167	arg1	diseases					183:190	autoimmune diseases	172:190	autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	172:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	17	theme	Fc-fusion	64:72	arg1	atacicept					82:90	The Fc-fusion protein atacicept	60:90	The Fc-fusion protein atacicept	60:90	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	18	theme	biotherapeutic	142:155	arg1	application					157:167	its biotherapeutic application	138:167	its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	138:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	5	19	theme	N-glycosylation	832:846	arg1	site					848:851	the N-glycosylation site	828:851	the N-glycosylation site microheterogeneity	828:870	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	4	20	theme	N-	712:713	arg1	sites					735:739	N- and O-glycosylation sites	712:739	N- and O-glycosylation sites	712:739	First, N- and O-glycosylation sites and their corresponding glycoforms were identified.					
31349756	1	21	theme	protein	74:80	arg1	atacicept					82:90	The Fc-fusion protein atacicept	60:90	The Fc-fusion protein atacicept	60:90	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	5	22	theme	N-glycans	967:975	arg1	analysis					942:949	analysis	942:949	analysis of the released N-glycans	942:975	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	2	23	theme	structural	512:521	arg1	diversity					523:531	structural diversity	512:531	structural diversity	512:531	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	3	24	theme	comprehensive	583:595	arg1	N-					611:612	comprehensive site-specific N-	583:612	comprehensive site-specific N-	583:612	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	0	25	theme	N-	14:15	arg1	analysis					37:44	Site-specific N- and O-glycosylation analysis	0:44	Site-specific N- and O-glycosylation analysis of atacicept	0:57	Site-specific N- and O-glycosylation analysis of atacicept.					
31349756	4	26	gly	O-glycosylation	719:733	arg2	sites					735:739	N- and O-glycosylation sites	712:739	N- and O-glycosylation sites	712:739	First, N- and O-glycosylation sites and their corresponding glycoforms were identified.					
31349756	2	27	theme	typical	320:326	arg1	therapeutics					350:361	typical recombinant IgG-based therapeutics	320:361	typical recombinant IgG-based therapeutics	320:361	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	3	28	theme	first	577:581	arg1	characterization					634:649	a first comprehensive site-specific N- and O-glycosylation characterization	575:649	a first comprehensive site-specific N- and O-glycosylation characterization of atacicept	575:662	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	2	29	theme	glycosylation-related	398:418	arg1	heterogeneity					420:432	heterogeneity	420:432	heterogeneity	420:432	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	0	30	theme	Site-specific	0:12	arg1	analysis					37:44	Site-specific N- and O-glycosylation analysis	0:44	Site-specific N- and O-glycosylation analysis of atacicept	0:57	Site-specific N- and O-glycosylation analysis of atacicept.					
31349756	1	31	theme	PRoliferation-Inducing	276:297	arg1	cytokines					229:237	the two cytokines	221:237	the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	221:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	31	theme	PRoliferation-Inducing	276:297	arg1	APRIL					307:311	APRIL	307:311	APRIL	307:311	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	31	theme	PRoliferation-Inducing	276:297	arg1	Ligand					299:304	A PRoliferation-Inducing Ligand	274:304	A PRoliferation-Inducing Ligand (APRIL)	274:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	4	32	theme	corresponding	751:763	arg1	glycoforms					765:774	their corresponding glycoforms	745:774	their corresponding glycoforms	745:774	First, N- and O-glycosylation sites and their corresponding glycoforms were identified.					
31349756	2	33	theme	site-specific	483:495	arg1	occupation					497:506	site-specific occupation	483:506	site-specific occupation	483:506	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	8	34	theme	production	1339:1348	arg1	batches					1350:1356	two individual production batches	1324:1356	two individual production batches	1324:1356	Overall, N- and O-glycosylation were consistent between two individual production batches.					
31349756	8	35	theme	individual	1328:1337	arg1	batches					1350:1356	two individual production batches	1324:1356	two individual production batches	1324:1356	Overall, N- and O-glycosylation were consistent between two individual production batches.					
31349756	7	36	theme	relative	1169:1176	arg1	distribution					1187:1198	The relative O-glycan distribution	1165:1198	The relative O-glycan distribution	1165:1198	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	2	37	gly	glycoprotein	379:390	arg1	atacicept					364:372	atacicept	364:372	atacicept	364:372	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	2	37	gly	glycoprotein	379:390	arg1	glycoprotein					379:390	a glycoprotein	377:390	a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans	377:555	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	1	38	theme	clinical	111:118	arg1	investigation					120:132	clinical investigation	111:132	clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	111:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	2	39	theme	glycosylation-site	450:467	arg1	localization					469:480	glycosylation-site localization	450:480	glycosylation-site localization	450:480	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	5	40	theme	glycopeptide	888:899	arg1	analysis					901:908	glycopeptide analysis	888:908	glycopeptide analysis	888:908	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	6	41	theme	different	1068:1076	arg1	compositions					1078:1089	34 different compositions	1065:1089	34 different compositions	1065:1089	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	5	42	theme	relative	803:810	arg1	quantitation					812:823	a relative quantitation	801:823	a relative quantitation of the N-glycosylation site microheterogeneity	801:870	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	6	43	with	sites	1133:1137	arg1	glycans					1156:1162	core 1-type glycans	1144:1162	core 1-type glycans	1144:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	44	contain	carrying	1033:1040	arg1	site					1027:1030	one N-glycosylation site	1007:1030	one N-glycosylation site	1007:1030	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	44	contain	carrying	1033:1040	arg2	glycoforms					1045:1054	47 glycoforms	1042:1054	47 glycoforms covering 34 different compositions	1042:1089	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	45	theme	34	1065:1066	arg1	compositions					1078:1089	34 different compositions	1065:1089	34 different compositions	1065:1089	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	7	46	theme	O-glycan	1178:1185	arg1	distribution					1187:1198	The relative O-glycan distribution	1165:1198	The relative O-glycan distribution	1165:1198	The relative O-glycan distribution was analyzed based on the de-N-glycosylated intact protein species.					
31349756	1	47	dep	cytokines	229:237	arg1	Stimulator					252:261	B-Lymphocyte Stimulator	239:261	B-Lymphocyte Stimulator (BLyS)	239:268	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	47	dep	cytokines	229:237	arg1	cytokines					229:237	the two cytokines	221:237	the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	221:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	47	dep	cytokines	229:237	arg1	APRIL					307:311	APRIL	307:311	APRIL	307:311	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	47	dep	cytokines	229:237	arg1	Ligand					299:304	A PRoliferation-Inducing Ligand	274:304	A PRoliferation-Inducing Ligand (APRIL)	274:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	47	dep	cytokines	229:237	arg1	BLyS					264:267	BLyS	264:267	BLyS	264:267	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	0	48	theme	O-glycosylation	21:35	arg1	analysis					37:44	Site-specific N- and O-glycosylation analysis	0:44	Site-specific N- and O-glycosylation analysis of atacicept	0:57	Site-specific N- and O-glycosylation analysis of atacicept.					
31349756	4	49	theme	O-glycosylation	719:733	arg1	sites					735:739	N- and O-glycosylation sites	712:739	N- and O-glycosylation sites	712:739	First, N- and O-glycosylation sites and their corresponding glycoforms were identified.					
31349756	6	50	theme	1-type	1149:1154	arg1	glycans					1156:1162	core 1-type glycans	1144:1162	core 1-type glycans	1144:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	3	51	theme	atacicept	654:662	arg1	characterization					634:649	a first comprehensive site-specific N- and O-glycosylation characterization	575:649	a first comprehensive site-specific N- and O-glycosylation characterization of atacicept	575:662	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	0	52	theme	atacicept	49:57	arg1	analysis					37:44	Site-specific N- and O-glycosylation analysis	0:44	Site-specific N- and O-glycosylation analysis of atacicept	0:57	Site-specific N- and O-glycosylation analysis of atacicept.					
31349756	0	53	gly	O-glycosylation	21:35	arg1	atacicept					49:57	atacicept	49:57	atacicept	49:57	Site-specific N- and O-glycosylation analysis of atacicept.					
31349756	3	54	theme	spectrometry-based	675:692	arg1	workflows					694:702	mass spectrometry-based workflows	670:702	mass spectrometry-based workflows	670:702	Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.					
31349756	2	55	theme	IgG-based	340:348	arg1	therapeutics					350:361	typical recombinant IgG-based therapeutics	320:361	typical recombinant IgG-based therapeutics	320:361	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	6	56	theme	core	1144:1147	arg1	glycans					1156:1162	core 1-type glycans	1144:1162	core 1-type glycans	1144:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	5	57	gly	glycopeptide	888:899	arg2	glycopeptide					888:899	glycopeptide analysis	888:908	glycopeptide analysis	888:908	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	2	58	theme	recombinant	328:338	arg1	therapeutics					350:361	typical recombinant IgG-based therapeutics	320:361	typical recombinant IgG-based therapeutics	320:361	Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans.					
31349756	1	59	theme	B-Lymphocyte	239:250	arg1	Stimulator					252:261	B-Lymphocyte Stimulator	239:261	B-Lymphocyte Stimulator (BLyS)	239:268	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	59	theme	B-Lymphocyte	239:250	arg1	cytokines					229:237	the two cytokines	221:237	the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL)	221:312	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	1	59	theme	B-Lymphocyte	239:250	arg1	BLyS					264:267	BLyS	264:267	BLyS	264:267	The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL).					
31349756	5	60	theme	microheterogeneity	853:870	arg1	quantitation					812:823	a relative quantitation	801:823	a relative quantitation of the N-glycosylation site microheterogeneity	801:870	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	5	61	theme	released	958:965	arg1	N-glycans					967:975	the released N-glycans	954:975	the released N-glycans	954:975	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	6	62	gly	O-glycosylation	1117:1131	arg2	sites					1133:1137	two hinge region O-glycosylation sites	1100:1137	two hinge region O-glycosylation sites with core 1-type glycans	1100:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	6	62	gly	O-glycosylation	1117:1131	arg2	two					1100:1102	two	1100:1102	two	1100:1102	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31349756	5	63	gly	N-glycosylation	832:846	arg2	site					848:851	the N-glycosylation site	828:851	the N-glycosylation site microheterogeneity	828:870	Second, a relative quantitation of the N-glycosylation site microheterogeneity was achieved by glycopeptide analysis, which was further supported by analysis of the released N-glycans.					
31349756	6	64	theme	O-glycosylation	1117:1131	arg1	sites					1133:1137	two hinge region O-glycosylation sites	1100:1137	two hinge region O-glycosylation sites with core 1-type glycans	1100:1162	We confirmed the presence of one N-glycosylation site, carrying 47 glycoforms covering 34 different compositions, next to two hinge region O-glycosylation sites with core 1-type glycans.					
31256374	1	0	theme	pure	156:159	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	1	contain	carry	59:63	arg2	mixture					86:92	a very heterogeneous mixture	65:92	a very heterogeneous mixture of oligosaccharides	65:112	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	1	contain	carry	59:63	arg1	glycoproteins					45:57	Most glycoproteins	40:57	Most glycoproteins	40:57	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	4	2	theme	strategies	890:899	arg1	part					876:879	a crucial part	866:879	a crucial part of these strategies	866:899	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	2	theme	strategies	890:899	arg1	separation					908:917	the separation	904:917	the separation of the glycan mixture into homogeneous glycan fractions	904:973	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	1	3	theme	glycoprotein	161:172	arg1	site					146:149	a single glycosylation site	123:149	a single glycosylation site of a pure glycoprotein	123:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	3	theme	glycoprotein	161:172	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	5	4	theme	chromatography	1021:1034	arg1	techniques					1043:1052	some high-performance liquid chromatography (HPLC) techniques	992:1052	some high-performance liquid chromatography (HPLC) techniques	992:1052	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	4	5	theme	specific	824:831	arg1	exoglycosidases					833:847	specific exoglycosidases	824:847	specific exoglycosidases	824:847	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	1	6	gly	glycosylated	199:210	arg1	site					146:149	a single glycosylation site	123:149	a single glycosylation site of a pure glycoprotein	123:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	6	gly	glycosylated	199:210	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	4	7	theme	sequence	800:807	arg1	analysis					809:816	sequence analysis	800:816	sequence analysis using specific exoglycosidases	800:847	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	8	theme	structural	622:631	arg1	characterization					633:648	the structural characterization	618:648	the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others	618:859	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	5	9	theme	high-performance	997:1012	arg1	HPLC					1037:1040	HPLC	1037:1040	HPLC	1037:1040	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	5	9	theme	high-performance	997:1012	arg1	chromatography					1021:1034	high-performance liquid chromatography	997:1034	some high-performance liquid chromatography (HPLC) techniques	992:1052	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	4	10	theme	linkage	734:740	arg1	analysis					742:749	linkage analysis	734:749	linkage analysis by gas chromatography-mass spectrometry (GC-MS)	734:797	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	1	11	theme	heterogeneous	72:84	arg1	mixture					86:92	a very heterogeneous mixture	65:92	a very heterogeneous mixture of oligosaccharides	65:112	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	2	12	theme	glycans	409:415	arg1	composition					394:404	the monosaccharide composition	375:404	the monosaccharide composition of glycans	375:415	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	4	13	theme	crucial	868:874	arg1	part					876:879	a crucial part	866:879	a crucial part of these strategies	866:899	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	13	theme	crucial	868:874	arg1	separation					908:917	the separation	904:917	the separation of the glycan mixture into homogeneous glycan fractions	904:973	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	2	14	from	differences	360:370	arg1	composition					394:404	the monosaccharide composition	375:404	the monosaccharide composition of glycans	375:415	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	3	15	theme	laboratory	548:557	arg1	investigation					559:571	laboratory investigation	548:571	laboratory investigation	548:571	Fortunately, the biosynthetic pathway is subject to certain restrictions, so that structural diversity is limited and amenable to laboratory investigation.					
31256374	2	16	theme	charges	331:337	arg1	number					321:326	number	321:326	number	321:326	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	2	16	theme	charges	331:337	arg1	size					312:315	size	312:315	size	312:315	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	3	17	theme	biosynthetic	435:446	arg1	subject					459:465	subject	459:465	subject	459:465	Fortunately, the biosynthetic pathway is subject to certain restrictions, so that structural diversity is limited and amenable to laboratory investigation.					
31256374	3	17	theme	biosynthetic	435:446	arg1	pathway					448:454	the biosynthetic pathway	431:454	the biosynthetic pathway	431:454	Fortunately, the biosynthetic pathway is subject to certain restrictions, so that structural diversity is limited and amenable to laboratory investigation.					
31256374	0	18	theme	Chromatographic	0:14	arg1	Profiling					16:24	Chromatographic Profiling	0:24	Chromatographic Profiling of N-Glycans.	0:38	Chromatographic Profiling of N-Glycans.					
31256374	1	19	theme	oligosaccharides	97:112	arg1	mixture					86:92	a very heterogeneous mixture	65:92	a very heterogeneous mixture of oligosaccharides	65:112	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	2	20	theme	monosaccharide	379:392	arg1	composition					394:404	the monosaccharide composition	375:404	the monosaccharide composition of glycans	375:415	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	0	21	theme	N-Glycans	29:37	arg1	Profiling					16:24	Chromatographic Profiling	0:24	Chromatographic Profiling of N-Glycans.	0:38	Chromatographic Profiling of N-Glycans.					
31256374	4	22	theme	Different	574:582	arg1	approaches					584:593	Different approaches	574:593	Different approaches	574:593	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	23	theme	homogeneous	946:956	arg1	fractions					965:973	homogeneous glycan fractions	946:973	homogeneous glycan fractions	946:973	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	24	theme	nuclear	681:687	arg1	NMR					709:711	NMR	709:711	NMR	709:711	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	24	theme	nuclear	681:687	arg1	resonance					698:706	nuclear magnetic resonance	681:706	nuclear magnetic resonance (NMR)	681:712	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	5	25	theme	liquid	1014:1019	arg1	HPLC					1037:1040	HPLC	1037:1040	HPLC	1037:1040	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	5	25	theme	liquid	1014:1019	arg1	chromatography					1021:1034	high-performance liquid chromatography	997:1034	some high-performance liquid chromatography (HPLC) techniques	992:1052	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	4	26	theme	glycan	958:963	arg1	fractions					965:973	homogeneous glycan fractions	946:973	homogeneous glycan fractions	946:973	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	5	27	theme	particular	1110:1119	arg1	glycans					1130:1136	particular N-linked glycans	1110:1136	particular N-linked glycans	1110:1136	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	1	28	gly	glycosylation	132:144	arg2	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	28	gly	glycosylation	132:144	arg2	site					146:149	a single glycosylation site	123:149	a single glycosylation site of a pure glycoprotein	123:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	28	gly	glycosylation	132:144	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	5	29	theme	N-linked	1121:1128	arg1	glycans					1130:1136	particular N-linked glycans	1110:1136	particular N-linked glycans	1110:1136	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	3	30	theme	certain	470:476	arg1	restrictions					478:489	certain restrictions	470:489	certain restrictions	470:489	Fortunately, the biosynthetic pathway is subject to certain restrictions, so that structural diversity is limited and amenable to laboratory investigation.					
31256374	2	31	theme	oligosaccharides	241:256	arg1	diversity					228:236	The structural diversity	213:236	The structural diversity of oligosaccharides	213:256	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	4	32	theme	mixture	933:939	arg1	part					876:879	a crucial part	866:879	a crucial part of these strategies	866:899	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	32	theme	mixture	933:939	arg1	separation					908:917	the separation	904:917	the separation of the glycan mixture into homogeneous glycan fractions	904:973	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	3	33	theme	structural	500:509	arg1	diversity					511:519	structural diversity	500:519	structural diversity	500:519	Fortunately, the biosynthetic pathway is subject to certain restrictions, so that structural diversity is limited and amenable to laboratory investigation.					
31256374	1	34	gly	glycoproteins	45:57	arg1	glycoproteins					45:57	Most glycoproteins	40:57	Most glycoproteins	40:57	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	35	theme	single	125:130	arg1	site					146:149	a single glycosylation site	123:149	a single glycosylation site of a pure glycoprotein	123:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	35	theme	single	125:130	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	4	36	theme	chromatography-mass	758:776	arg1	GC-MS					792:796	GC-MS	792:796	GC-MS	792:796	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	36	theme	chromatography-mass	758:776	arg1	spectrometry					778:789	gas chromatography-mass spectrometry	754:789	gas chromatography-mass spectrometry (GC-MS)	754:797	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	1	37	gly	glycoprotein	161:172	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	2	38	theme	structural	217:226	arg1	diversity					228:236	The structural diversity	213:236	The structural diversity of oligosaccharides	213:256	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	1	39	theme	glycosylation	132:144	arg1	site					146:149	a single glycosylation site	123:149	a single glycosylation site of a pure glycoprotein	123:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	1	39	theme	glycosylation	132:144	arg1	glycoprotein					161:172	a pure glycoprotein	154:172	a pure glycoprotein	154:172	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	2	40	from	differences	293:303	arg1	number					321:326	number	321:326	number	321:326	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	2	40	from	differences	293:303	arg1	size					312:315	size	312:315	size	312:315	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	4	41	theme	glycan	926:931	arg1	mixture					933:939	the glycan mixture	922:939	the glycan mixture	922:939	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	5	42	theme	oligosaccharides	1089:1104	arg1	isolation					1076:1084	the isolation	1072:1084	the isolation of oligosaccharides	1072:1104	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	1	43	theme	Most	40:43	arg1	glycoproteins					45:57	Most glycoproteins	40:57	Most glycoproteins	40:57	Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often heterogeneously glycosylated.					
31256374	4	44	theme	gas	754:756	arg1	GC-MS					792:796	GC-MS	792:796	GC-MS	792:796	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	44	theme	gas	754:756	arg1	spectrometry					778:789	gas chromatography-mass spectrometry	754:789	gas chromatography-mass spectrometry (GC-MS)	754:797	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	2	45	theme	glycans	342:348	arg1	charges					331:337	charges	331:337	charges of glycans	331:348	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	4	46	theme	magnetic	689:696	arg1	NMR					709:711	NMR	709:711	NMR	709:711	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	4	46	theme	magnetic	689:696	arg1	resonance					698:706	nuclear magnetic resonance	681:706	nuclear magnetic resonance (NMR)	681:712	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	2	47	theme	linkage	270:276	arg1	variants					278:285	linkage variants	270:285	linkage variants	270:285	The structural diversity of oligosaccharides arises from linkage variants, from differences in the size and number of charges of glycans, and from differences in the monosaccharide composition of glycans.					
31256374	4	48	theme	mass	715:718	arg1	spectrometry					720:731	mass spectrometry	715:731	mass spectrometry	715:731	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
31256374	5	49	link	N-linked	1121:1128	arg1	glycans					1130:1136	particular N-linked glycans	1110:1136	particular N-linked glycans	1110:1136	In this chapter some high-performance liquid chromatography (HPLC) techniques are described for the isolation of oligosaccharides, in particular N-linked glycans.					
31256374	4	50	theme	oligosaccharides	653:668	arg1	characterization					633:648	the structural characterization	618:648	the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others	618:859	Different approaches have been developed to the structural characterization of oligosaccharides, including nuclear magnetic resonance (NMR), mass spectrometry, linkage analysis by gas chromatography-mass spectrometry (GC-MS), sequence analysis using specific exoglycosidases, and others, but a crucial part of these strategies is the separation of the glycan mixture into homogeneous glycan fractions.					
30934974	8	0	located	located	955:961	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	0	located	located	955:961	arg1	ratios					983:988	the fycosylation ratios	966:988	the fycosylation ratios	966:988	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	0	located	located	955:961	arg1	bi-					1014:1016	bi-	1014:1016	bi-	1014:1016	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	0	located	located	955:961	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	0	located	located	955:961	arg1	amount					1004:1009	the total amount	994:1009	the total amount of bi- and tetra-antennary structures (all p < 0.0001)	994:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	0	located	located	955:961	arg2	differences					938:948	These differences	932:948	These differences	932:948	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	9	1	theme	Gleason	1119:1125	arg1	scores					1127:1132	various Gleason scores	1111:1132	various Gleason scores	1111:1132	These differences were also present between various Gleason scores.					
30934974	3	2	from	patients	448:455	arg1	specimens					429:437	METHODS Tissue specimens	414:437	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50)	414:516	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	12	3	from	changes	1430:1436	arg1	profile					1454:1460	the N-glycan profile	1441:1460	the N-glycan profile of prostate tissue	1441:1479	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	4	4	theme	N-glycosylation	584:598	arg1	profile					600:606	a tissue N-glycosylation profile	575:606	a tissue N-glycosylation profile	575:606	The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined.					
30934974	7	5	dep	RESULTS	823:829	arg1	observed					848:855	observed	848:855	was observed for the tissue of N-glycosylation between the benign and malignant tissue	844:929	RESULTS A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue.					
30934974	2	6	theme	prostate	280:287	arg1	tissue					289:294	prostate tissue	280:294	prostate tissue	280:294	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	8	7	from	ratios	983:988	arg1	located					955:961	located	955:961	located	955:961	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	11	8	theme	spectral	1251:1258	arg1	ranges					1260:1265	spectral ranges	1251:1265	spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm	1251:1298	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	12	9	theme	malignant	1523:1531	arg1	tissue					1544:1549	malignant and benign tissue	1523:1549	malignant and benign tissue	1523:1549	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	5	10	theme	formalin-fixed	642:655	arg1	slides					675:680	the formalin-fixed paraffin-embedded slides	638:680	the formalin-fixed paraffin-embedded slides	638:680	Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy.					
30934974	8	11	from	amount	1004:1009	arg1	located					955:961	located	955:961	located	955:961	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	11	12	theme	nm	1280:1281	arg1	ranges					1260:1265	spectral ranges	1251:1265	spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm	1251:1298	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	3	13	theme	=	512:512	arg1	n					510:510	n = 50	510:515	n = 50	510:515	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	14	14	theme	further	1762:1768	arg1	validation					1777:1786	further method validation	1762:1786	further method validation	1762:1786	This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.					
30934974	9	15	theme	various	1111:1117	arg1	scores					1127:1132	various Gleason scores	1111:1132	various Gleason scores	1111:1132	These differences were also present between various Gleason scores.					
30934974	3	16	theme	=	495:495	arg1	n					493:493	n = 50	493:498	n = 50	493:498	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	13	17	located	observed	1642:1649	arg2	changes					1634:1640	the changes	1630:1640	the changes observed in the NIR spectra	1630:1668	These changes can be correlated to the changes observed in the NIR spectra.					
30934974	13	17	located	observed	1642:1649	arg1	spectra					1662:1668	the NIR spectra	1654:1668	the NIR spectra	1654:1668	These changes can be correlated to the changes observed in the NIR spectra.					
30934974	5	18	theme	NIR	702:704	arg1	spectroscopy					706:717	NIR spectroscopy	702:717	NIR spectroscopy	702:717	Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy.					
30934974	7	19	theme	malignant	914:922	arg1	tissue					924:929	the benign and malignant tissue	899:929	the benign and malignant tissue	899:929	RESULTS A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue.					
30934974	14	20	theme	PCa	1738:1740	arg1	diagnosis					1742:1750	PCa diagnosis	1738:1750	PCa diagnosis	1738:1750	This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.					
30934974	12	21	theme	tissue	1474:1479	arg1	profile					1454:1460	the N-glycan profile	1441:1460	the N-glycan profile of prostate tissue	1441:1479	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	0	22	theme	Cancer	83:88	arg1	Diagnosis					61:69	the Diagnosis	57:69	the Diagnosis of Prostate Cancer	57:88	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	7	23	theme	benign	903:908	arg1	tissue					924:929	the benign and malignant tissue	899:929	the benign and malignant tissue	899:929	RESULTS A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue.					
30934974	12	24	theme	N-glycan	1445:1452	arg1	profile					1454:1460	the N-glycan profile	1441:1460	the N-glycan profile of prostate tissue	1441:1479	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	2	25	theme	spectroscopy	377:388	arg1	possibilities					319:331	the possibilities	315:331	the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis	315:411	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	12	26	theme	various	1571:1577	arg1	scores					1587:1592	various Gleason scores	1571:1592	various Gleason scores	1571:1592	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	0	27	from	Spectroscopy	41:52	arg1	Diagnosis					61:69	the Diagnosis	57:69	the Diagnosis of Prostate Cancer	57:88	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	0	28	theme	N-Linked	0:7	arg1	Glycosylation					9:21	N-Linked Glycosylation	0:21	N-Linked Glycosylation	0:21	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	14	29	theme	histological	1711:1722	arg1	assessment					1724:1733	the histological assessment	1707:1733	the histological assessment of PCa diagnosis	1707:1750	This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.					
30934974	8	30	theme	bi-	1014:1016	arg1	ratios					983:988	the fycosylation ratios	966:988	the fycosylation ratios	966:988	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	30	theme	bi-	1014:1016	arg1	bi-					1014:1016	bi-	1014:1016	bi-	1014:1016	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	30	theme	bi-	1014:1016	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	30	theme	bi-	1014:1016	arg1	amount					1004:1009	the total amount	994:1009	the total amount of bi- and tetra-antennary structures (all p < 0.0001)	994:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	30	theme	bi-	1014:1016	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	6	31	theme	Gleason	807:813	arg1	scores					815:820	the various Gleason scores	795:820	the various Gleason scores	795:820	A comparison was made between the benign and malignant tissue, and between the various Gleason scores.					
30934974	1	32	theme	prostate	115:122	arg1	biopsy					124:129	a prostate biopsy	113:129	a prostate biopsy	113:129	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	1	33	theme	robust	143:148	arg1	BACKGROUND					91:100	BACKGROUND	91:100	BACKGROUND Performing a prostate biopsy	91:129	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	1	33	theme	robust	143:148	arg1	way					163:165	the most robust and reliable way	134:165	the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading	134:237	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	10	34	theme	several	1224:1230	arg1	regions					1232:1238	several regions	1224:1238	several regions	1224:1238	In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions.					
30934974	3	35	theme	Tissue	422:427	arg1	specimens					429:437	METHODS Tissue specimens	414:437	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50)	414:516	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	8	36	theme	tetra-antennary	1022:1036	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	36	theme	tetra-antennary	1022:1036	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	3	37	theme	benign	458:463	arg1	patients					448:455	100 patients	444:455	100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50)	444:516	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	37	theme	benign	458:463	arg1	BPH					487:489	BPH	487:489	BPH	487:489	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	37	theme	benign	458:463	arg1	hyperplasia					474:484	benign prostate hyperplasia	458:484	benign prostate hyperplasia (BPH)	458:490	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	8	38	theme	p	1054:1054	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	38	theme	p	1054:1054	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	2	39	theme	tissue	336:341	arg1	N-glycosylation					343:357	tissue N-glycosylation	336:357	tissue N-glycosylation	336:357	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	8	40	from	located	955:961	arg1	ratios					983:988	the fycosylation ratios	966:988	the fycosylation ratios	966:988	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	40	from	located	955:961	arg1	bi-					1014:1016	bi-	1014:1016	bi-	1014:1016	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	40	from	located	955:961	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	40	from	located	955:961	arg1	amount					1004:1009	the total amount	994:1009	the total amount of bi- and tetra-antennary structures (all p < 0.0001)	994:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	40	from	located	955:961	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	11	41	theme	nm	1297:1298	arg1	ranges					1260:1265	spectral ranges	1251:1265	spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm	1251:1298	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	1	42	theme	disease	223:229	arg1	grading					231:237	the disease grading	219:237	the disease grading	219:237	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	11	43	theme	Gleason	1341:1347	arg1	p					1372:1372	p	1372:1372	p	1372:1372	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	43	theme	Gleason	1341:1347	arg1	p					1357:1357	p	1357:1357	p = 0.0042	1357:1366	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	43	theme	Gleason	1341:1347	arg1	scores					1349:1354	the Gleason scores	1337:1354	the Gleason scores (p = 0.0042 and p = 0.0195)	1337:1382	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	6	44	theme	benign	754:759	arg1	tissue					775:780	the benign and malignant tissue	750:780	the benign and malignant tissue	750:780	A comparison was made between the benign and malignant tissue, and between the various Gleason scores.					
30934974	2	45	theme	biochemical	256:266	arg1	markers					268:274	little to no biochemical markers	243:274	little to no biochemical markers for prostate tissue	243:294	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	2	46	from	possibilities	319:331	arg1	diagnosis					403:411	PCa diagnosis	399:411	PCa diagnosis	399:411	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	1	47	theme	prostate	179:186	arg1	PCa					196:198	PCa	196:198	PCa	196:198	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	1	47	theme	prostate	179:186	arg1	cancer					188:193	prostate cancer	179:193	prostate cancer (PCa)	179:199	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	4	48	theme	tissue	577:582	arg1	profile					600:606	a tissue N-glycosylation profile	575:606	a tissue N-glycosylation profile	575:606	The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined.					
30934974	13	49	theme	NIR	1658:1660	arg1	spectra					1662:1668	the NIR spectra	1654:1668	the NIR spectra	1654:1668	These changes can be correlated to the changes observed in the NIR spectra.					
30934974	4	50	theme	fresh-frozen	537:548	arg1	tissue					550:555	The fresh-frozen tissue	533:555	The fresh-frozen tissue	533:555	The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined.					
30934974	14	51	theme	method	1770:1775	arg1	validation					1777:1786	further method validation	1762:1786	further method validation	1762:1786	This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.					
30934974	10	52	from	tissue	1214:1219	arg1	regions					1232:1238	several regions	1224:1238	several regions	1224:1238	In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions.					
30934974	11	53	dep	=	1359:1359	arg1	0.0042					1361:1366	0.0042	1361:1366	0.0042	1361:1366	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	12	54	theme	prostate	1465:1472	arg1	tissue					1474:1479	prostate tissue	1465:1479	prostate tissue	1465:1479	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	11	55	dep	scores	1349:1354	arg1	p					1372:1372	p	1372:1372	p	1372:1372	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	55	dep	scores	1349:1354	arg1	p					1357:1357	p	1357:1357	p = 0.0042	1357:1366	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	55	dep	scores	1349:1354	arg1	scores					1349:1354	the Gleason scores	1337:1354	the Gleason scores (p = 0.0042 and p = 0.0195)	1337:1382	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	10	56	theme	NIR	1152:1154	arg1	spectra					1156:1162	the NIR spectra	1148:1162	the NIR spectra	1148:1162	In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions.					
30934974	12	57	dep	CONCLUSIONS	1385:1395	arg1	demonstrated					1405:1416	demonstrated	1405:1416	have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores	1400:1592	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	0	58	theme	Prostate	74:81	arg1	Cancer					83:88	Prostate Cancer	74:88	Prostate Cancer	74:88	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	14	59	theme	diagnosis	1742:1750	arg1	assessment					1724:1733	the histological assessment	1707:1733	the histological assessment of PCa diagnosis	1707:1750	This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.					
30934974	5	60	dep	formalin-fixed	642:655	arg1	paraffin-embedded					657:673	paraffin-embedded	657:673	paraffin-embedded	657:673	Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy.					
30934974	11	61	dep	=	1374:1374	arg1	0.0195					1376:1381	0.0195	1376:1381	0.0195	1376:1381	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	2	62	dep	little	243:248	arg1	to					250:251	to	250:251	to	250:251	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	10	63	theme	benign	1193:1198	arg1	tissue					1214:1219	the benign and malignant tissue	1189:1219	the benign and malignant tissue in several regions	1189:1238	In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions.					
30934974	8	64	theme	fycosylation	970:981	arg1	ratios					983:988	the fycosylation ratios	966:988	the fycosylation ratios	966:988	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	1	65	theme	reliable	154:161	arg1	BACKGROUND					91:100	BACKGROUND	91:100	BACKGROUND Performing a prostate biopsy	91:129	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	1	65	theme	reliable	154:161	arg1	way					163:165	the most robust and reliable way	134:165	the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading	134:237	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	12	66	theme	Gleason	1579:1585	arg1	scores					1587:1592	various Gleason scores	1571:1592	various Gleason scores	1571:1592	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	0	67	from	Glycosylation	9:21	arg1	Diagnosis					61:69	the Diagnosis	57:69	the Diagnosis of Prostate Cancer	57:88	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	11	68	theme	=	1359:1359	arg1	p					1357:1357	p	1357:1357	p = 0.0042	1357:1366	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	68	theme	=	1359:1359	arg1	scores					1349:1354	the Gleason scores	1337:1354	the Gleason scores (p = 0.0042 and p = 0.0195)	1337:1382	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	8	69	theme	total	998:1002	arg1	bi-					1014:1016	bi-	1014:1016	bi-	1014:1016	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	69	theme	total	998:1002	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	69	theme	total	998:1002	arg1	amount					1004:1009	the total amount	994:1009	the total amount of bi- and tetra-antennary structures (all p < 0.0001)	994:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	69	theme	total	998:1002	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	10	70	theme	malignant	1204:1212	arg1	tissue					1214:1219	the benign and malignant tissue	1189:1219	the benign and malignant tissue in several regions	1189:1238	In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions.					
30934974	3	71	theme	METHODS	414:420	arg1	specimens					429:437	METHODS Tissue specimens	414:437	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50)	414:516	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	7	72	theme	N-glycosylation	875:889	arg1	tissue					865:870	the tissue	861:870	the tissue of N-glycosylation between the benign and malignant tissue	861:929	RESULTS A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue.					
30934974	3	73	dep	hyperplasia	474:484	arg1	n					510:510	n = 50	510:515	n = 50	510:515	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	73	dep	hyperplasia	474:484	arg1	PCa					505:507	PCa	505:507	PCa	505:507	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	73	dep	hyperplasia	474:484	arg1	n					493:493	n = 50	493:498	n = 50	493:498	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	1	74	dep	way	163:165	arg1	determine					209:217	determine	209:217	to determine the disease grading	206:237	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	1	74	dep	way	163:165	arg1	diagnose					170:177	diagnose	170:177	to diagnose prostate cancer (PCa)	167:199	BACKGROUND Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading.					
30934974	11	75	theme	=	1374:1374	arg1	p					1357:1357	p	1357:1357	p = 0.0042	1357:1366	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	11	75	theme	=	1374:1374	arg1	scores					1349:1354	the Gleason scores	1337:1354	the Gleason scores (p = 0.0042 and p = 0.0195)	1337:1382	Moreover, spectral ranges of 1055⁻1065 nm and 1450⁻1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).					
30934974	2	76	theme	N-glycosylation	343:357	arg1	possibilities					319:331	the possibilities	315:331	the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis	315:411	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	0	77	theme	Near-Infrared	27:39	arg1	Spectroscopy					41:52	Near-Infrared Spectroscopy	27:52	Near-Infrared Spectroscopy	27:52	N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.					
30934974	12	78	theme	benign	1537:1542	arg1	tissue					1544:1549	malignant and benign tissue	1523:1549	malignant and benign tissue	1523:1549	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	2	79	theme	PCa	399:401	arg1	diagnosis					403:411	PCa diagnosis	399:411	PCa diagnosis	399:411	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	8	80	theme	structures	1038:1047	arg1	ratios					983:988	the fycosylation ratios	966:988	the fycosylation ratios	966:988	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	80	theme	structures	1038:1047	arg1	bi-					1014:1016	bi-	1014:1016	bi-	1014:1016	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	80	theme	structures	1038:1047	arg1	structures					1038:1047	tetra-antennary structures	1022:1047	tetra-antennary structures (all p < 0.0001)	1022:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	80	theme	structures	1038:1047	arg1	amount					1004:1009	the total amount	994:1009	the total amount of bi- and tetra-antennary structures (all p < 0.0001)	994:1064	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	8	80	theme	structures	1038:1047	arg1	<					1056:1056	all p < 0.0001	1050:1063	all p < 0.0001	1050:1063	These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001).					
30934974	6	81	theme	various	799:805	arg1	scores					815:820	the various Gleason scores	795:820	the various Gleason scores	795:820	A comparison was made between the benign and malignant tissue, and between the various Gleason scores.					
30934974	12	82	theme	biochemical	1418:1428	arg1	changes					1430:1436	biochemical changes	1418:1436	biochemical changes	1418:1436	CONCLUSIONS We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores.					
30934974	3	83	theme	prostate	465:472	arg1	patients					448:455	100 patients	444:455	100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50)	444:516	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	83	theme	prostate	465:472	arg1	BPH					487:489	BPH	487:489	BPH	487:489	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	3	83	theme	prostate	465:472	arg1	hyperplasia					474:484	benign prostate hyperplasia	458:484	benign prostate hyperplasia (BPH)	458:490	METHODS Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained.					
30934974	6	84	theme	malignant	765:773	arg1	tissue					775:780	the benign and malignant tissue	750:780	the benign and malignant tissue	750:780	A comparison was made between the benign and malignant tissue, and between the various Gleason scores.					
30934974	2	85	theme	near-infrared	363:375	arg1	NIR					391:393	NIR	391:393	NIR	391:393	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
30934974	2	85	theme	near-infrared	363:375	arg1	spectroscopy					377:388	near-infrared spectroscopy	363:388	near-infrared spectroscopy (NIR)	363:394	As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.					
31393126	6	0	theme	N-glycopeptides	937:951	arg1	glycosylation					994:1006	site-specific glycosylation	980:1006	site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time	980:1089	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	0	theme	N-glycopeptides	937:951	arg1	total					925:929	A total	923:929	A total of 50 N-glycopeptides and 30 N-glycans	923:968	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	2	1	theme	RJ	329:330	arg1	proteins					332:339	RJ proteins	329:339	RJ proteins	329:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	7	2	theme	N-glycans	1119:1127	arg1	N-glycans					1119:1127	the identified N-glycans	1104:1127	the identified N-glycans	1104:1127	Eighteen of the identified N-glycans have been structurally characterized by IT-MS, including oligosaccharide composition, sequence, branching, and linkage.					
31393126	7	2	theme	N-glycans	1119:1127	arg1	Eighteen					1092:1099	Eighteen	1092:1099	Eighteen	1092:1099	Eighteen of the identified N-glycans have been structurally characterized by IT-MS, including oligosaccharide composition, sequence, branching, and linkage.					
31393126	3	3	theme	glycomics	472:480	arg1	strategy					502:509	a combined glycomics and glycoproteomics strategy	461:509	a combined glycomics and glycoproteomics strategy	461:509	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	1	4	theme	human	244:248	arg1	health					250:255	human health	244:255	human health	244:255	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	8	5	theme	N-glycosylation	1253:1267	arg1	sites					1269:1273	Two N-glycosylation sites	1249:1273	Two N-glycosylation sites (N177 and N394)	1249:1289	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	5	theme	N-glycosylation	1253:1267	arg1	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	5	theme	N-glycosylation	1253:1267	arg1	N394					1285:1288	N394	1285:1288	N394	1285:1288	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	5	theme	N-glycosylation	1253:1267	arg1	N177					1276:1279	N177	1276:1279	N177	1276:1279	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	11	6	theme	glycosylation	1730:1742	arg1	heterogeneity					1744:1756	the site-specific glycosylation heterogeneity	1712:1756	the site-specific glycosylation heterogeneity of the RJ glycoproteins	1712:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	3	7	theme	N-linked	559:566	arg1	heterogeneity					582:594	N-linked glycosylation heterogeneity	559:594	N-linked glycosylation heterogeneity of RJ glycoproteins	559:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	3	8	theme	heterogeneity	582:594	arg1	analysis					547:554	the site-specific analysis	529:554	the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins	529:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	5	9	theme	liquid	848:853	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	9	theme	liquid	848:853	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	6	10	theme	site-specific	980:992	arg1	glycosylation					994:1006	site-specific glycosylation	980:1006	site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time	980:1089	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	10	theme	site-specific	980:992	arg1	total					925:929	A total	923:929	A total of 50 N-glycopeptides and 30 N-glycans	923:968	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	11	11	theme	desirable	1842:1850	arg1	activity					1883:1890	desirable pharmacokinetic and biological activity	1842:1890	desirable pharmacokinetic and biological activity	1842:1890	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	3	12	gly	heterogeneity	582:594	arg1	glycoproteins					602:614	RJ glycoproteins	599:614	RJ glycoproteins	599:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	11	13	theme	RJ	1765:1766	arg1	glycoproteins					1768:1780	the RJ glycoproteins	1761:1780	the RJ glycoproteins	1761:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	3	14	theme	RJ	599:600	arg1	glycoproteins					602:614	RJ glycoproteins	599:614	RJ glycoproteins	599:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	1	15	from	development	176:186	arg1	honeybees					218:226	honeybees	218:226	honeybees	218:226	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	5	16	theme	trap-sequential	874:888	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	16	theme	trap-sequential	874:888	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	4	17	theme	ion	709:711	arg1	IT-MS					748:752	IT-MS	748:752	IT-MS	748:752	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	4	17	theme	ion	709:711	arg1	spectrometry					734:745	direct infusion ion trap-sequential mass spectrometry	693:745	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	10	18	theme	N-glycans	1503:1511	arg1	diversity					1490:1498	The diversity	1486:1498	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins	1486:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	19	attach	attached	1513:1520	arg1	site					1551:1554	each single glycosylation site	1525:1554	each single glycosylation site of these glycoproteins	1525:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	19	attach	attached	1513:1520	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	19	attach	attached	1513:1520	arg2	N-glycans					1503:1511	N-glycans	1503:1511	N-glycans attached to each single glycosylation site of these glycoproteins	1503:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	4	20	theme	mass	729:732	arg1	IT-MS					748:752	IT-MS	748:752	IT-MS	748:752	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	4	20	theme	mass	729:732	arg1	spectrometry					734:745	direct infusion ion trap-sequential mass spectrometry	693:745	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	1	21	from	function	206:213	arg1	honeybees					218:226	honeybees	218:226	honeybees	218:226	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	1	22	theme	young	127:131	arg1	bees					140:143	young worker bees	127:143	young worker bees	127:143	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	0	23	theme	Jelly	74:78	arg1	Glycoproteins					80:92	Royal Jelly Glycoproteins	68:92	Royal Jelly Glycoproteins	68:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	4	24	theme	direct	693:698	arg1	IT-MS					748:752	IT-MS	748:752	IT-MS	748:752	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	4	24	theme	direct	693:698	arg1	spectrometry					734:745	direct infusion ion trap-sequential mass spectrometry	693:745	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	11	25	with	glycoprotein	1824:1835	arg1	activity					1883:1890	desirable pharmacokinetic and biological activity	1842:1890	desirable pharmacokinetic and biological activity	1842:1890	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	6	26	theme	jelly	1032:1036	arg1	protein					1038:1044	major royal jelly protein 1	1020:1046	major royal jelly protein 1 (MRJP1)	1020:1054	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	26	theme	jelly	1032:1036	arg1	MRJP1					1049:1053	MRJP1	1049:1053	MRJP1	1049:1053	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	27	theme	major	1020:1024	arg1	protein					1038:1044	major royal jelly protein 1	1020:1046	major royal jelly protein 1 (MRJP1)	1020:1054	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	27	theme	major	1020:1024	arg1	MRJP1					1049:1053	MRJP1	1049:1053	MRJP1	1049:1053	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	11	28	theme	pharmacokinetic	1852:1866	arg1	activity					1883:1890	desirable pharmacokinetic and biological activity	1842:1890	desirable pharmacokinetic and biological activity	1842:1890	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	0	29	theme	Heterogeneity	49:61	arg1	Analysis					14:21	Site-Specific Analysis	0:21	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.	0:93	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	1	30	theme	Royal	95:99	arg1	RJ					108:109	RJ	108:109	RJ	108:109	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	1	30	theme	Royal	95:99	arg1	jelly					101:105	Royal jelly	95:105	Royal jelly (RJ)	95:110	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	6	31	theme	first	1080:1084	arg1	time					1086:1089	the first time	1076:1089	the first time	1076:1089	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	2	32	theme	glycosylation	415:427	arg1	analysis					403:410	a site-specific analysis	387:410	a site-specific analysis of glycosylation from these glycoproteins	387:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	2	33	from	glycoproteins	440:452	arg1	glycosylation					415:427	glycosylation	415:427	glycosylation from these glycoproteins	415:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	2	33	from	glycoproteins	440:452	arg1	analysis					403:410	a site-specific analysis	387:410	a site-specific analysis of glycosylation from these glycoproteins	387:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	10	34	theme	glycosylation	1537:1549	arg1	site					1551:1554	each single glycosylation site	1525:1554	each single glycosylation site of these glycoproteins	1525:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	34	theme	glycosylation	1537:1549	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	0	35	theme	Site-Specific	0:12	arg1	Analysis					14:21	Site-Specific Analysis	0:21	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.	0:93	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	8	36	dep	sites	1269:1273	arg1	sites					1269:1273	Two N-glycosylation sites	1249:1273	Two N-glycosylation sites (N177 and N394)	1249:1289	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	36	dep	sites	1269:1273	arg1	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	36	dep	sites	1269:1273	arg1	N394					1285:1288	N394	1285:1288	N394	1285:1288	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	36	dep	sites	1269:1273	arg1	N177					1276:1279	N177	1276:1279	N177	1276:1279	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	5	37	theme	hydrophilic	824:834	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	37	theme	hydrophilic	824:834	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	11	38	theme	biological	1872:1881	arg1	activity					1883:1890	desirable pharmacokinetic and biological activity	1842:1890	desirable pharmacokinetic and biological activity	1842:1890	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	11	39	gly	glycoproteins	1768:1780	arg1	glycoproteins					1768:1780	the RJ glycoproteins	1761:1780	the RJ glycoproteins	1761:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	10	40	theme	glycoproteins	1565:1577	arg1	site					1551:1554	each single glycosylation site	1525:1554	each single glycosylation site of these glycoproteins	1525:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	40	theme	glycoproteins	1565:1577	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	0	41	theme	Glycosylation	35:47	arg1	Heterogeneity					49:61	N-Linked Glycosylation Heterogeneity	26:61	N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins	26:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	1	42	dep	development	176:186	arg1	the					172:174	the	172:174	the	172:174	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	11	43	gly	heterogeneity	1744:1756	arg1	glycoproteins					1768:1780	the RJ glycoproteins	1761:1780	the RJ glycoproteins	1761:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	2	44	theme	glycosylation	299:311	arg1	modification					313:324	the glycosylation modification	295:324	the glycosylation modification of RJ proteins	295:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	1	45	theme	physiological	192:204	arg1	function					206:213	physiological function	192:213	physiological function	192:213	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	8	46	dep	sites	1294:1298	arg1	sites					1294:1298	3 sites	1292:1298	3 sites (N145, N178, and N92)	1292:1320	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	46	dep	sites	1294:1298	arg1	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	46	dep	sites	1294:1298	arg1	N178					1307:1310	N178	1307:1310	N178	1307:1310	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	46	dep	sites	1294:1298	arg1	N92					1317:1319	N92	1317:1319	N92	1317:1319	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	46	dep	sites	1294:1298	arg1	N145					1301:1304	N145	1301:1304	N145	1301:1304	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	3	47	theme	glycoproteomics	486:500	arg1	strategy					502:509	a combined glycomics and glycoproteomics strategy	461:509	a combined glycomics and glycoproteomics strategy	461:509	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	2	48	theme	proteins	332:339	arg1	modification					313:324	the glycosylation modification	295:324	the glycosylation modification of RJ proteins	295:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	7	49	theme	identified	1108:1117	arg1	N-glycans					1119:1127	the identified N-glycans	1104:1127	the identified N-glycans	1104:1127	Eighteen of the identified N-glycans have been structurally characterized by IT-MS, including oligosaccharide composition, sequence, branching, and linkage.					
31393126	10	50	theme	glycoform	1657:1665	arg1	populations					1667:1677	their glycoform populations	1651:1677	their glycoform populations	1651:1677	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	5	51	theme	mass	890:893	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	51	theme	mass	890:893	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	4	52	theme	N-glycome	655:663	arg1	characterization					631:646	global characterization	624:646	global characterization of the N-glycome of RJ	624:669	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	1	53	theme	key	159:161	arg1	roles					163:167	key roles	159:167	key roles	159:167	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	3	54	gly	glycoproteins	602:614	arg1	glycoproteins					602:614	RJ glycoproteins	599:614	RJ glycoproteins	599:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	11	55	theme	heterogeneity	1744:1756	arg1	properties					1698:1707	the properties	1694:1707	the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins	1694:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	3	56	theme	site-specific	533:545	arg1	analysis					547:554	the site-specific analysis	529:554	the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins	529:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	5	57	theme	interaction	836:846	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	57	theme	interaction	836:846	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	11	58	gly	glycoprotein	1824:1835	arg1	glycoprotein					1824:1835	a glycoprotein	1822:1835	a glycoprotein with desirable pharmacokinetic and biological activity	1822:1890	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	4	59	theme	global	624:629	arg1	characterization					631:646	global characterization	624:646	global characterization of the N-glycome of RJ	624:669	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	3	60	theme	combined	463:470	arg1	strategy					502:509	a combined glycomics and glycoproteomics strategy	461:509	a combined glycomics and glycoproteomics strategy	461:509	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg2	N177					1276:1279	N177	1276:1279	N177	1276:1279	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg2	N394					1285:1288	N394	1285:1288	N394	1285:1288	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg2	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg2	Two					1249:1251	Two	1249:1251	Two	1249:1251	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg1	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	61	gly	N-glycosylation	1253:1267	arg2	sites					1269:1273	Two N-glycosylation sites	1249:1273	Two N-glycosylation sites (N177 and N394)	1249:1289	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	4	62	theme	RJ	668:669	arg1	N-glycome					655:663	the N-glycome	651:663	the N-glycome of RJ	651:669	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	5	63	theme	chromatography-ion	855:872	arg1	spectrometry					895:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry	824:906	hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS)	824:920	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	5	63	theme	chromatography-ion	855:872	arg1	HILIC-IT-MS					909:919	HILIC-IT-MS	909:919	HILIC-IT-MS	909:919	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	0	64	from	Glycoproteins	80:92	arg1	Heterogeneity					49:61	N-Linked Glycosylation Heterogeneity	26:61	N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins	26:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	0	64	from	Glycoproteins	80:92	arg1	Analysis					14:21	Site-Specific Analysis	0:21	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.	0:93	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	3	65	gly	glycosylation	568:580	arg1	glycoproteins					602:614	RJ glycoproteins	599:614	RJ glycoproteins	599:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	11	66	theme	glycoproteins	1768:1780	arg1	heterogeneity					1744:1756	the site-specific glycosylation heterogeneity	1712:1756	the site-specific glycosylation heterogeneity of the RJ glycoproteins	1712:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	10	67	gly	glycoproteins	1565:1577	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	3	68	theme	glycosylation	568:580	arg1	heterogeneity					582:594	N-linked glycosylation heterogeneity	559:594	N-linked glycosylation heterogeneity of RJ glycoproteins	559:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	6	69	theme	N-glycans	960:968	arg1	glycosylation					994:1006	site-specific glycosylation	980:1006	site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time	980:1089	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	69	theme	N-glycans	960:968	arg1	total					925:929	A total	923:929	A total of 50 N-glycopeptides and 30 N-glycans	923:968	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	4	70	theme	infusion	700:707	arg1	IT-MS					748:752	IT-MS	748:752	IT-MS	748:752	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	4	70	theme	infusion	700:707	arg1	spectrometry					734:745	direct infusion ion trap-sequential mass spectrometry	693:745	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	5	71	gly	glycopeptides	779:791	arg2	glycopeptides					779:791	tryptic glycopeptides	771:791	tryptic glycopeptides	771:791	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	4	72	theme	trap-sequential	713:727	arg1	IT-MS					748:752	IT-MS	748:752	IT-MS	748:752	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	4	72	theme	trap-sequential	713:727	arg1	spectrometry					734:745	direct infusion ion trap-sequential mass spectrometry	693:745	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	10	73	gly	glycosylation	1537:1549	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	73	gly	glycosylation	1537:1549	arg2	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	73	gly	glycosylation	1537:1549	arg2	site					1551:1554	each single glycosylation site	1525:1554	each single glycosylation site of these glycoproteins	1525:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	3	74	theme	glycoproteins	602:614	arg1	heterogeneity					582:594	N-linked glycosylation heterogeneity	559:594	N-linked glycosylation heterogeneity of RJ glycoproteins	559:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	1	75	theme	worker	133:138	arg1	bees					140:143	young worker bees	127:143	young worker bees	127:143	Royal jelly (RJ) is secreted by young worker bees, and it plays key roles in the development and physiological function in honeybees and can improve human health.					
31393126	2	76	from	analysis	403:410	arg1	glycoproteins					440:452	these glycoproteins	434:452	these glycoproteins	434:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	6	77	gly	N-glycopeptides	937:951	arg2	N-glycopeptides					937:951	50 N-glycopeptides and 30 N-glycans	934:968	N-glycopeptides	937:951	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	3	78	link	N-linked	559:566	arg1	heterogeneity					582:594	N-linked glycosylation heterogeneity	559:594	N-linked glycosylation heterogeneity of RJ glycoproteins	559:614	Here, a combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity of RJ glycoproteins.					
31393126	0	79	from	Analysis	14:21	arg1	Glycoproteins					80:92	Royal Jelly Glycoproteins	68:92	Royal Jelly Glycoproteins	68:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	2	80	from	analyses	283:290	arg1	modification					313:324	the glycosylation modification	295:324	the glycosylation modification of RJ proteins	295:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	6	81	theme	royal	1026:1030	arg1	protein					1038:1044	major royal jelly protein 1	1020:1046	major royal jelly protein 1 (MRJP1)	1020:1054	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	81	theme	royal	1026:1030	arg1	MRJP1					1049:1053	MRJP1	1049:1053	MRJP1	1049:1053	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	7	82	theme	oligosaccharide	1186:1200	arg1	composition					1202:1212	oligosaccharide composition	1186:1212	oligosaccharide composition	1186:1212	Eighteen of the identified N-glycans have been structurally characterized by IT-MS, including oligosaccharide composition, sequence, branching, and linkage.					
31393126	11	83	theme	site-specific	1716:1728	arg1	heterogeneity					1744:1756	the site-specific glycosylation heterogeneity	1712:1756	the site-specific glycosylation heterogeneity of the RJ glycoproteins	1712:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	4	84	theme	spectrometry	734:745	arg1	method					755:760	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	a direct infusion ion trap-sequential mass spectrometry (IT-MS) method	691:760	First, global characterization of the N-glycome of RJ was performed using a direct infusion ion trap-sequential mass spectrometry (IT-MS) method.					
31393126	10	85	theme	single	1530:1535	arg1	site					1551:1554	each single glycosylation site	1525:1554	each single glycosylation site of these glycoproteins	1525:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	10	85	theme	single	1530:1535	arg1	glycoproteins					1565:1577	these glycoproteins	1559:1577	these glycoproteins	1559:1577	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	0	86	theme	N-Linked	26:33	arg1	Heterogeneity					49:61	N-Linked Glycosylation Heterogeneity	26:61	N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins	26:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	6	87	theme	RJ	1069:1070	arg1	protein					1038:1044	major royal jelly protein 1	1020:1046	major royal jelly protein 1 (MRJP1)	1020:1054	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	87	theme	RJ	1069:1070	arg1	MRJP1					1049:1053	MRJP1	1049:1053	MRJP1	1049:1053	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	6	87	theme	RJ	1069:1070	arg1	MRJP2					1060:1064	MRJP2	1060:1064	MRJP2 of RJ for the first time	1060:1089	A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time.					
31393126	2	88	theme	site-specific	389:401	arg1	analysis					403:410	a site-specific analysis	387:410	a site-specific analysis of glycosylation from these glycoproteins	387:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	5	89	theme	tryptic	771:777	arg1	glycopeptides					779:791	tryptic glycopeptides	771:791	tryptic glycopeptides	771:791	Second, tryptic glycopeptides were enriched and separated by hydrophilic interaction liquid chromatography-ion trap-sequential mass spectrometry (HILIC-IT-MS).					
31393126	2	90	gly	glycoproteins	440:452	arg1	glycoproteins					440:452	these glycoproteins	434:452	these glycoproteins	434:452	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	8	91	theme	N183	1337:1340	arg1	N178					1307:1310	N178	1307:1310	N178	1307:1310	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	sites					1269:1273	Two N-glycosylation sites	1249:1273	Two N-glycosylation sites (N177 and N394)	1249:1289	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	site					1329:1332	1 site	1327:1332	1 site of N183	1327:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	sites					1294:1298	3 sites	1292:1298	3 sites (N145, N178, and N92)	1292:1320	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	N92					1317:1319	N92	1317:1319	N92	1317:1319	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	N177					1276:1279	N177	1276:1279	N177	1276:1279	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	N183					1337:1340	N183	1337:1340	N183	1337:1340	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	N394					1285:1288	N394	1285:1288	N394	1285:1288	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	8	91	theme	N183	1337:1340	arg1	N145					1301:1304	N145	1301:1304	N145	1301:1304	Two N-glycosylation sites (N177 and N394), 3 sites (N145, N178, and N92), and 1 site of N183 were identified in MRJP1, MRJP2, and MRJP3, respectively.					
31393126	0	92	theme	Royal	68:72	arg1	Glycoproteins					80:92	Royal Jelly Glycoproteins	68:92	Royal Jelly Glycoproteins	68:92	Site-Specific Analysis of N-Linked Glycosylation Heterogeneity from Royal Jelly Glycoproteins.					
31393126	10	93	theme	MRJP2	1604:1608	arg1	heterogeneity					1610:1622	MRJP2 heterogeneity	1604:1622	MRJP2 heterogeneity	1604:1622	The diversity of N-glycans attached to each single glycosylation site of these glycoproteins confirmed that MRJP1 and MRJP2 heterogeneity was mostly associated with their glycoform populations.					
31393126	9	94	attach	attached	1435:1442	arg1	MRJP2					1454:1458	MRJP2	1454:1458	MRJP2	1454:1458	There were 18, 17, and 2 N-glycans attached to MRJP1, MRJP2, and MRJP3, respectively.					
31393126	9	94	attach	attached	1435:1442	arg1	MRJP3					1465:1469	MRJP3	1465:1469	MRJP3	1465:1469	There were 18, 17, and 2 N-glycans attached to MRJP1, MRJP2, and MRJP3, respectively.					
31393126	9	94	attach	attached	1435:1442	arg2	N-glycans					1425:1433	18, 17, and 2 N-glycans	1411:1433	18, 17, and 2 N-glycans attached to MRJP1, MRJP2, and MRJP3, respectively	1411:1483	There were 18, 17, and 2 N-glycans attached to MRJP1, MRJP2, and MRJP3, respectively.					
31393126	9	94	attach	attached	1435:1442	arg1	MRJP1					1447:1451	MRJP1	1447:1451	MRJP1	1447:1451	There were 18, 17, and 2 N-glycans attached to MRJP1, MRJP2, and MRJP3, respectively.					
31393126	11	95	gly	glycosylation	1730:1742	arg1	glycoproteins					1768:1780	the RJ glycoproteins	1761:1780	the RJ glycoproteins	1761:1780	Understanding the properties of the site-specific glycosylation heterogeneity of the RJ glycoproteins can be potentially useful for producing a glycoprotein with desirable pharmacokinetic and biological activity.					
31393126	2	96	mod	modification	313:324	arg1	proteins					332:339	RJ proteins	329:339	RJ proteins	329:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
31393126	2	96	mod	modification	313:324	arg3	glycosylation					299:311	the glycosylation modification	295:324	the glycosylation modification of RJ proteins	295:339	Although there have been analyses on the glycosylation modification of RJ proteins, none of these methods have been conducted on a site-specific analysis of glycosylation from these glycoproteins.					
30904681	0	0	theme	intact	89:94	arg1	B2GP1					116:120	B2GP1	116:120	B2GP1	116:120	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	0	theme	intact	89:94	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	6	1	theme	glycan	905:910	arg1	composition					912:922	The dominant glycan composition	892:922	The dominant glycan composition	892:922	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	7	2	theme	lower	1105:1109	arg1	amounts					1111:1117	lower amounts	1105:1117	lower amounts	1105:1117	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	3	3	theme	different	492:500	arg1	variants					510:517	Next to its glycosylation different genetic variants	466:517	Next to its glycosylation different genetic variants	466:517	Next to its glycosylation different genetic variants are known to increase the risk of suffering from APS.					
30904681	1	4	theme	plasma	239:244	arg1	protein					246:252	a 50 kDa human plasma protein	224:252	a 50 kDa human plasma protein with four or five N-glycosylation sites	224:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	4	theme	plasma	239:244	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	2	5	gly	Glycosylation	295:307	arg1	B2GP1					312:316	B2GP1	312:316	B2GP1	312:316	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	0	6	from	characterization	13:28	arg1	sites					80:84	multiple glycosylation sites	57:84	multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1)	57:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	6	from	characterization	13:28	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	3	7	theme	genetic	502:508	arg1	variants					510:517	Next to its glycosylation different genetic variants	466:517	Next to its glycosylation different genetic variants	466:517	Next to its glycosylation different genetic variants are known to increase the risk of suffering from APS.					
30904681	9	8	theme	human	1405:1409	arg1	B2GP1					1411:1415	human B2GP1	1405:1415	human B2GP1	1405:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	5	9	theme	B2GP1	714:718	arg1	enrichment					700:709	enrichment	700:709	enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein	700:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	10	from	measurement	775:785	arg1	enrichment					700:709	enrichment	700:709	enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein	700:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	0	11	gly	glycosylation	66:78	arg1	B2GP1					116:120	B2GP1	116:120	B2GP1	116:120	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	11	gly	glycosylation	66:78	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	11	gly	glycosylation	66:78	arg2	sites					80:84	multiple glycosylation sites	57:84	multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1)	57:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	11	gly	glycosylation	66:78	arg2	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	9	12	theme	B2GP1	1411:1415	arg1	variants					1369:1376	genetic variants	1361:1376	genetic variants	1361:1376	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	12	theme	B2GP1	1411:1415	arg1	compositions					1389:1400	glycan compositions	1382:1400	glycan compositions	1382:1400	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	0	13	theme	β-2-glycoprotein-1	96:113	arg1	sites					80:84	multiple glycosylation sites	57:84	multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1)	57:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	13	theme	β-2-glycoprotein-1	96:113	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	5	14	theme	heterozygous	878:889	arg1	samples					849:855	our samples	845:855	our samples either homozygous or heterozygous	845:889	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	9	15	theme	diagnostic	1443:1452	arg1	marker					1454:1459	diagnostic marker	1443:1459	diagnostic marker	1443:1459	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	15	theme	diagnostic	1443:1452	arg1	determination					1344:1356	a fast determination	1337:1356	a fast determination of genetic variants and glycan compositions of human B2GP1	1337:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	7	16	theme	triantennary	1071:1082	arg1	structures					1091:1100	one or two triantennary glycan structures	1060:1100	one or two triantennary glycan structures in lower amounts	1060:1117	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	4	17	theme	intact	607:612	arg1	B2GP1					614:618	intact B2GP1	607:618	intact B2GP1	607:618	Here we show that ESI-q/TOF-MS of intact B2GP1 can be used to analyze genetic variants and glycosylation simultaneously.					
30904681	7	18	with	species	1135:1141	arg1	residues					1167:1174	one or two fucosyl residues	1148:1174	one or two fucosyl residues	1148:1174	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	8	19	theme	fifth	1236:1240	arg1	site					1256:1259	an unreported partially occupied fifth glycosylation site	1203:1259	an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N	1203:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	2	20	theme	syndrome	402:409	arg1	development					370:380	the development	366:380	the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis	366:463	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	5	21	theme	different	728:736	arg1	samples					745:751	16 different plasma samples	725:751	16 different plasma samples	725:751	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	22	theme	ESI-MS	768:773	arg1	measurement					775:785	subsequent ESI-MS measurement	757:785	subsequent ESI-MS measurement of the intact protein	757:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	23	theme	protein	801:807	arg1	samples					745:751	16 different plasma samples	725:751	16 different plasma samples	725:751	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	23	theme	protein	801:807	arg1	measurement					775:785	subsequent ESI-MS measurement	757:785	subsequent ESI-MS measurement of the intact protein	757:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	2	24	theme	antiphospholipid	385:400	arg1	APS					412:414	APS	412:414	APS	412:414	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	2	24	theme	antiphospholipid	385:400	arg1	syndrome					402:409	antiphospholipid syndrome	385:409	antiphospholipid syndrome (APS)	385:415	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	5	25	theme	intact	794:799	arg1	protein					801:807	the intact protein	790:807	the intact protein	790:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	26	theme	subsequent	757:766	arg1	measurement					775:785	subsequent ESI-MS measurement	757:785	subsequent ESI-MS measurement of the intact protein	757:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	2	27	theme	B2GP1	312:316	arg1	Glycosylation					295:307	Glycosylation	295:307	Glycosylation of B2GP1	295:316	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	4	28	theme	genetic	643:649	arg1	variants					651:658	genetic variants	643:658	genetic variants	643:658	Here we show that ESI-q/TOF-MS of intact B2GP1 can be used to analyze genetic variants and glycosylation simultaneously.					
30904681	0	29	theme	Simultaneous	0:11	arg1	characterization					13:28	Simultaneous characterization	0:28	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.	0:137	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	8	30	gly	glycosylation	1242:1254	arg2	site					1256:1259	an unreported partially occupied fifth glycosylation site	1203:1259	an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N	1203:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	6	31	theme	relative	991:998	arg1	proportion					1000:1009	a relative proportion	989:1009	a relative proportion of about 30%	989:1022	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	8	32	theme	occupied	1227:1234	arg1	site					1256:1259	an unreported partially occupied fifth glycosylation site	1203:1259	an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N	1203:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	0	33	theme	SNPs	33:36	arg1	characterization					13:28	Simultaneous characterization	0:28	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.	0:137	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	6	34	theme	glycan	965:970	arg1	structures					972:981	four biantennary, fully sialylated glycan structures	930:981	four biantennary, fully sialylated glycan structures	930:981	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	8	35	theme	samples	1188:1194	arg1	Two					1177:1179	Two	1177:1179	Two	1177:1179	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	8	35	theme	samples	1188:1194	arg1	samples					1188:1194	our samples	1184:1194	our samples	1184:1194	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	9	36	used	used	1435:1438	arg2	determination					1344:1356	a fast determination	1337:1356	a fast determination of genetic variants and glycan compositions of human B2GP1	1337:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	36	used	used	1435:1438	arg2	marker					1454:1459	diagnostic marker	1443:1459	diagnostic marker	1443:1459	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	1	37	theme	called	196:201	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	37	theme	called	196:201	arg1	H					218:218	also called apolipoprotein H	191:218	also called apolipoprotein H	191:218	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	38	theme	N-glycosylation	272:286	arg1	sites					288:292	four or five N-glycosylation sites	259:292	four or five N-glycosylation sites	259:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	8	39	theme	S88N	1313:1316	arg1	presence					1289:1296	the presence	1285:1296	the presence of SNP variant S88N	1285:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	1	40	theme	apolipoprotein	203:216	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	40	theme	apolipoprotein	203:216	arg1	H					218:218	also called apolipoprotein H	191:218	also called apolipoprotein H	191:218	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	0	41	theme	N-glycans	42:50	arg1	characterization					13:28	Simultaneous characterization	0:28	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.	0:137	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	7	42	theme	fucosyl	1159:1165	arg1	residues					1167:1174	one or two fucosyl residues	1148:1174	one or two fucosyl residues	1148:1174	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	8	43	theme	glycosylation	1242:1254	arg1	site					1256:1259	an unreported partially occupied fifth glycosylation site	1203:1259	an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N	1203:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	5	44	theme	different	827:835	arg1	SNPs					837:840	five different SNPs	822:840	five different SNPs	822:840	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	8	45	theme	unreported	1206:1215	arg1	site					1256:1259	an unreported partially occupied fifth glycosylation site	1203:1259	an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N	1203:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	7	46	from	structures	1091:1100	arg1	amounts					1111:1117	lower amounts	1105:1117	lower amounts	1105:1117	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	0	47	from	sites	80:84	arg1	N-glycans					42:50	N-glycans	42:50	N-glycans	42:50	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	47	from	sites	80:84	arg1	SNPs					33:36	SNPs	33:36	SNPs	33:36	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	47	from	sites	80:84	arg1	characterization					13:28	Simultaneous characterization	0:28	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.	0:137	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	9	48	theme	glycan	1382:1387	arg1	compositions					1389:1400	glycan compositions	1382:1400	glycan compositions	1382:1400	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	6	49	theme	%	1022:1022	arg1	proportion					1000:1009	a relative proportion	989:1009	a relative proportion of about 30%	989:1022	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	0	50	theme	multiple	57:64	arg1	sites					80:84	multiple glycosylation sites	57:84	multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1)	57:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	50	theme	multiple	57:64	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	6	51	theme	biantennary	935:945	arg1	structures					972:981	four biantennary, fully sialylated glycan structures	930:981	four biantennary, fully sialylated glycan structures	930:981	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	1	52	gly	glycosylated	150:161	arg1	protein					246:252	a 50 kDa human plasma protein	224:252	a 50 kDa human plasma protein with four or five N-glycosylation sites	224:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	52	gly	glycosylated	150:161	arg1	B2GP1					183:187	B2GP1	183:187	B2GP1	183:187	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	52	gly	glycosylated	150:161	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	52	gly	glycosylated	150:161	arg1	H					218:218	also called apolipoprotein H	191:218	also called apolipoprotein H	191:218	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	6	53	dep	biantennary	935:945	arg1	sialylated					954:963	sialylated	954:963	sialylated	954:963	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	7	54	gly	fucosylated	1123:1133	arg1	species					1135:1141	fucosylated species	1123:1141	fucosylated species with one or two fucosyl residues	1123:1174	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	7	55	with	structures	1091:1100	arg1	residues					1167:1174	one or two fucosyl residues	1148:1174	one or two fucosyl residues	1148:1174	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	2	56	theme	antibody	334:341	arg1	recognition					343:353	auto antibody recognition	329:353	auto antibody recognition	329:353	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	7	57	theme	fucosylated	1123:1133	arg1	species					1135:1141	fucosylated species	1123:1141	fucosylated species with one or two fucosyl residues	1123:1174	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	6	58	theme	dominant	896:903	arg1	composition					912:922	The dominant glycan composition	892:922	The dominant glycan composition	892:922	The dominant glycan composition shows four biantennary, fully sialylated glycan structures, with a relative proportion of about 30%.					
30904681	7	59	theme	glycan	1084:1089	arg1	structures					1091:1100	one or two triantennary glycan structures	1060:1100	one or two triantennary glycan structures in lower amounts	1060:1117	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	4	60	used	used	627:630	arg2	ESI-q/TOF-MS					591:602	ESI-q/TOF-MS	591:602	ESI-q/TOF-MS of intact B2GP1	591:618	Here we show that ESI-q/TOF-MS of intact B2GP1 can be used to analyze genetic variants and glycosylation simultaneously.					
30904681	2	61	theme	auto	329:332	arg1	recognition					343:353	auto antibody recognition	329:353	auto antibody recognition	329:353	Glycosylation of B2GP1 can impact auto antibody recognition leading to the development of antiphospholipid syndrome (APS), which can result in miscarriages or thrombosis.					
30904681	9	62	theme	genetic	1361:1367	arg1	variants					1369:1376	genetic variants	1361:1376	genetic variants	1361:1376	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	1	63	gly	N-glycosylation	272:286	arg2	four					259:262	four	259:262	four	259:262	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	63	gly	N-glycosylation	272:286	arg2	sites					288:292	four or five N-glycosylation sites	259:292	four or five N-glycosylation sites	259:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	63	gly	N-glycosylation	272:286	arg2	five					267:270	five	267:270	five	267:270	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	9	64	theme	variants	1369:1376	arg1	determination					1344:1356	a fast determination	1337:1356	a fast determination of genetic variants and glycan compositions of human B2GP1	1337:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	64	theme	variants	1369:1376	arg1	marker					1454:1459	diagnostic marker	1443:1459	diagnostic marker	1443:1459	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	1	65	with	protein	246:252	arg1	sites					288:292	four or five N-glycosylation sites	259:292	four or five N-glycosylation sites	259:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	8	66	theme	variant	1305:1311	arg1	S88N					1313:1316	SNP variant S88N	1301:1316	SNP variant S88N	1301:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	8	67	theme	SNP	1301:1303	arg1	S88N					1313:1316	SNP variant S88N	1301:1316	SNP variant S88N	1301:1316	Two of our samples showed an unreported partially occupied fifth glycosylation site presumably arising from the presence of SNP variant S88N.					
30904681	9	68	theme	fast	1339:1342	arg1	determination					1344:1356	a fast determination	1337:1356	a fast determination of genetic variants and glycan compositions of human B2GP1	1337:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	68	theme	fast	1339:1342	arg1	marker					1454:1459	diagnostic marker	1443:1459	diagnostic marker	1443:1459	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	5	69	theme	plasma	738:743	arg1	samples					745:751	16 different plasma samples	725:751	16 different plasma samples	725:751	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	5	70	from	samples	745:751	arg1	enrichment					700:709	enrichment	700:709	enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein	700:807	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	4	71	theme	B2GP1	614:618	arg1	ESI-q/TOF-MS					591:602	ESI-q/TOF-MS	591:602	ESI-q/TOF-MS of intact B2GP1	591:618	Here we show that ESI-q/TOF-MS of intact B2GP1 can be used to analyze genetic variants and glycosylation simultaneously.					
30904681	5	72	theme	homozygous	864:873	arg1	samples					849:855	our samples	845:855	our samples either homozygous or heterozygous	845:889	After enrichment of B2GP1 from 16 different plasma samples and subsequent ESI-MS measurement of the intact protein, we detected five different SNPs in our samples either homozygous or heterozygous.					
30904681	7	73	from	species	1135:1141	arg1	amounts					1111:1117	lower amounts	1105:1117	lower amounts	1105:1117	We also detected compositions with one or two triantennary glycan structures in lower amounts and fucosylated species with one or two fucosyl residues.					
30904681	1	74	theme	50 kDa	226:231	arg1	protein					246:252	a 50 kDa human plasma protein	224:252	a 50 kDa human plasma protein with four or five N-glycosylation sites	224:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	74	theme	50 kDa	226:231	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	0	75	theme	glycosylation	66:78	arg1	sites					80:84	multiple glycosylation sites	57:84	multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1)	57:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	0	75	theme	glycosylation	66:78	arg1	β-2-glycoprotein-1					96:113	intact β-2-glycoprotein-1	89:113	intact β-2-glycoprotein-1 (B2GP1)	89:121	Simultaneous characterization of SNPs and N-glycans from multiple glycosylation sites of intact β-2-glycoprotein-1 (B2GP1) by ESI-qTOF-MS.					
30904681	9	76	theme	compositions	1389:1400	arg1	determination					1344:1356	a fast determination	1337:1356	a fast determination of genetic variants and glycan compositions of human B2GP1	1337:1415	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	9	76	theme	compositions	1389:1400	arg1	marker					1454:1459	diagnostic marker	1443:1459	diagnostic marker	1443:1459	Our method allows a fast determination of genetic variants and glycan compositions of human B2GP1 to be potentially used as diagnostic marker.					
30904681	1	77	theme	glycosylated	150:161	arg1	protein					246:252	a 50 kDa human plasma protein	224:252	a 50 kDa human plasma protein with four or five N-glycosylation sites	224:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	77	theme	glycosylated	150:161	arg1	B2GP1					183:187	B2GP1	183:187	B2GP1	183:187	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	77	theme	glycosylated	150:161	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	77	theme	glycosylated	150:161	arg1	H					218:218	also called apolipoprotein H	191:218	also called apolipoprotein H	191:218	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	78	theme	human	233:237	arg1	protein					246:252	a 50 kDa human plasma protein	224:252	a 50 kDa human plasma protein with four or five N-glycosylation sites	224:292	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
30904681	1	78	theme	human	233:237	arg1	ß-2-glycoprotein-1					163:180	The highly glycosylated ß-2-glycoprotein-1	139:180	The highly glycosylated ß-2-glycoprotein-1 (B2GP1)	139:188	The highly glycosylated ß-2-glycoprotein-1 (B2GP1), also called apolipoprotein H, is a 50 kDa human plasma protein with four or five N-glycosylation sites.					
29321476	8	0	theme	snap	1022:1025	arg1	frozen					1027:1032	snap frozen	1022:1032	snap frozen	1022:1032	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	2	1	dep	signatures	297:306	arg1	alter					308:312	alter	308:312	alter rapidly after tissue excision or during long-term storage	308:370	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	10	2	theme	group	1281:1285	arg1	ABO					1287:1289	blood group ABO	1275:1289	blood group ABO in the normal area	1275:1308	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	0	3	theme	gastric	69:75	arg1	cancer					77:82	paired gastric cancer	62:82	paired gastric cancer	62:82	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	0	4	from	handling	7:14	arg1	cancer					77:82	paired gastric cancer	62:82	paired gastric cancer	62:82	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	0	4	from	handling	7:14	arg1	tissues					104:110	non-tumorigenic tissues	88:110	non-tumorigenic tissues	88:110	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	8	5	theme	preservation	1087:1098	arg1	comparable					1113:1122	comparable	1113:1122	comparable	1113:1122	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	8	5	theme	preservation	1087:1098	arg1	methods					1100:1106	the two preservation methods	1079:1106	the two preservation methods	1079:1106	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	8	6	theme	heat-stabilized	1038:1052	arg1	samples					1054:1060	heat-stabilized samples	1038:1060	heat-stabilized samples	1038:1060	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	7	7	used	used	924:927	arg2	abundance					858:866	the relative abundance	845:866	the relative abundance of 83 O-glycans and 17 derived structural features	845:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	10	8	theme	blood	1275:1279	arg1	ABO					1287:1289	blood group ABO	1275:1289	blood group ABO in the normal area	1275:1308	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	0	9	theme	non-tumorigenic	88:102	arg1	tissues					104:110	non-tumorigenic tissues	88:110	non-tumorigenic tissues	88:110	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	4	10	theme	Paired	551:556	arg1	tumor					558:562	Paired tumor and adjacent normal tissue samples	551:597	tumor	558:562	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
29321476	4	11	theme	gastric	642:648	arg1	surgery					657:663	gastric cancer surgery	642:663	gastric cancer surgery	642:663	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
29321476	7	12	link	derived	891:897	arg1	features					910:917	17 derived structural features	888:917	17 derived structural features	888:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	10	13	from	ABO	1287:1289	arg1	area					1305:1308	the normal area	1294:1308	the normal area	1294:1308	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	2	14	theme	protein	289:295	arg1	results					278:284	the results	274:284	the results	274:284	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	2	14	theme	protein	289:295	arg1	signatures					297:306	protein signatures	289:306	protein signatures alter rapidly after tissue excision or during long-term storage	289:370	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	7	15	theme	features	910:917	arg1	abundance					858:866	the relative abundance	845:866	the relative abundance of 83 O-glycans and 17 derived structural features	845:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	2	16	theme	long-term	354:362	arg1	storage					364:370	long-term storage	354:370	long-term storage	354:370	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	10	17	theme	LacdiNAc	1337:1344	arg1	epitope					1346:1352	the LacdiNAc epitope	1333:1352	the LacdiNAc epitope (N,N'-diacetyllactosamine)	1333:1379	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	5	18	theme	Collected	666:674	arg1	n = 24					685:690	n = 24	685:690	n = 24	685:690	Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized.					
29321476	5	18	theme	Collected	666:674	arg1	samples					676:682	Collected samples	666:682	Collected samples (n = 24)	666:691	Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized.					
29321476	9	19	theme	significant	1146:1156	arg1	changes					1158:1164	significant changes	1146:1164	significant changes between normal and cancerous tissue	1146:1200	The data also showed significant changes between normal and cancerous tissue.					
29321476	1	20	theme	most	161:164	arg1	steps					175:179	the most critical steps	157:179	the most critical steps for ensuring the highest preservation of specimens	157:230	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	20	theme	most	161:164	arg1	storage					145:151	storage	145:151	storage	145:151	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	20	theme	most	161:164	arg1	handling					132:139	handling	132:139	handling	132:139	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	20	theme	most	161:164	arg1	collection					120:129	Sample collection	113:129	Sample collection	113:129	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	0	21	theme	O-glycan	38:45	arg1	alterations					47:57	O-glycan alterations	38:57	O-glycan alterations	38:57	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	7	22	theme	derived	891:897	arg1	features					910:917	17 derived structural features	888:917	17 derived structural features	888:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	3	23	theme	state-of-the-art	401:416	arg1	methods					439:445	current state-of-the-art biobank preservation methods	393:445	current state-of-the-art biobank preservation methods	393:445	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	3	24	theme	gastric	527:533	arg1	tissues					542:548	the gastric cancer tissues	523:548	the gastric cancer tissues	523:548	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	1	25	theme	critical	166:173	arg1	steps					175:179	the most critical steps	157:179	the most critical steps for ensuring the highest preservation of specimens	157:230	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	25	theme	critical	166:173	arg1	storage					145:151	storage	145:151	storage	145:151	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	25	theme	critical	166:173	arg1	handling					132:139	handling	132:139	handling	132:139	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	25	theme	critical	166:173	arg1	collection					120:129	Sample collection	113:129	Sample collection	113:129	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	3	26	theme	current	393:399	arg1	methods					439:445	current state-of-the-art biobank preservation methods	393:445	current state-of-the-art biobank preservation methods	393:445	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	11	27	theme	O-glycan	1432:1439	arg1	alterations					1441:1451	The O-glycan alterations	1428:1451	The O-glycan alterations that are presented here	1428:1475	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	2	28	theme	tissue	328:333	arg1	excision					335:342	tissue excision	328:342	tissue excision	328:342	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	7	29	theme	structural	899:908	arg1	features					910:917	17 derived structural features	888:917	17 derived structural features	888:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	0	30	theme	Sample	0:5	arg1	handling					7:14	Sample handling	0:14	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.	0:111	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	10	31	from	shift	1220:1224	arg1	area					1262:1265	the cancer area	1251:1265	the cancer area	1251:1265	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	9	32	theme	normal	1174:1179	arg1	tissue					1195:1200	normal and cancerous tissue	1174:1200	normal and cancerous tissue	1174:1200	The data also showed significant changes between normal and cancerous tissue.					
29321476	3	33	theme	glycomics	454:462	arg1	perspective					464:474	a glycomics perspective	452:474	a glycomics perspective	452:474	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	0	34	theme	gastric	19:25	arg1	tissue					27:32	gastric tissue	19:32	gastric tissue	19:32	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	11	35	theme	predictive	1489:1498	arg1	power					1500:1504	predictive power	1489:1504	predictive power for the detection and prognosis of gastric cancer	1489:1554	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	11	36	theme	gastric	1541:1547	arg1	cancer					1549:1554	gastric cancer	1541:1554	gastric cancer	1541:1554	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	5	37	dep	either	698:703	arg1	snap-frozen					705:715	snap-frozen	705:715	snap-frozen	705:715	Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized.					
29321476	5	37	dep	either	698:703	arg1	stabilized					725:734	stabilized	725:734	stabilized	725:734	Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized.					
29321476	5	37	dep	either	698:703	arg1	homogenized					745:755	homogenized	745:755	homogenized	745:755	Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized.					
29321476	8	38	located	found	995:999	arg2	difference					984:993	no statistically significant difference	955:993	no statistically significant difference	955:993	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	8	38	located	found	995:999	arg1	variables					1004:1012	variables	1004:1012	variables between snap frozen and heat-stabilized samples	1004:1060	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	10	39	from	sialylation	1236:1246	arg1	shift					1220:1224	a shift	1218:1224	a shift from high sialylation in the cancer area towards blood group ABO in the normal area	1218:1308	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	9	40	theme	cancerous	1185:1193	arg1	tissue					1195:1200	normal and cancerous tissue	1174:1200	normal and cancerous tissue	1174:1200	The data also showed significant changes between normal and cancerous tissue.					
29321476	11	41	dep	detection	1514:1522	arg1	the					1510:1512	the	1510:1512	the	1510:1512	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	7	42	theme	relative	849:856	arg1	abundance					858:866	the relative abundance	845:866	the relative abundance of 83 O-glycans and 17 derived structural features	845:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	2	43	theme	Pre-analytical	233:246	arg1	variability					248:258	Pre-analytical variability	233:258	Pre-analytical variability	233:258	Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage.					
29321476	7	44	theme	O-glycans	874:882	arg1	abundance					858:866	the relative abundance	845:866	the relative abundance of 83 O-glycans and 17 derived structural features	845:917	In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison.					
29321476	1	45	theme	highest	198:204	arg1	preservation					206:217	the highest preservation	194:217	the highest preservation of specimens	194:230	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	0	46	theme	tissue	27:32	arg1	handling					7:14	Sample handling	0:14	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.	0:111	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	4	47	theme	adjacent	568:575	arg1	samples					591:597	Paired tumor and adjacent normal tissue samples	551:597	samples	591:597	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
29321476	6	48	attach	released	771:778	arg2	Glycans					758:764	Glycans	758:764	Glycans	758:764	Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS.					
29321476	6	48	attach	released	771:778	arg1	glycoproteins					795:807	extracted glycoproteins	785:807	extracted glycoproteins	785:807	Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS.					
29321476	3	49	theme	O-glycan	489:496	arg1	alterations					498:508	O-glycan alterations	489:508	O-glycan alterations occurring in the gastric cancer tissues	489:548	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	0	50	theme	alterations	47:57	arg1	handling					7:14	Sample handling	0:14	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.	0:111	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	10	51	theme	high	1231:1234	arg1	sialylation					1236:1246	high sialylation	1231:1246	high sialylation	1231:1246	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	8	52	theme	significant	972:982	arg1	difference					984:993	no statistically significant difference	955:993	no statistically significant difference	955:993	There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable.					
29321476	1	53	theme	Sample	113:118	arg1	steps					175:179	the most critical steps	157:179	the most critical steps for ensuring the highest preservation of specimens	157:230	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	53	theme	Sample	113:118	arg1	storage					145:151	storage	145:151	storage	145:151	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	53	theme	Sample	113:118	arg1	handling					132:139	handling	132:139	handling	132:139	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	1	53	theme	Sample	113:118	arg1	collection					120:129	Sample collection	113:129	Sample collection	113:129	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	3	54	theme	preservation	426:437	arg1	methods					439:445	current state-of-the-art biobank preservation methods	393:445	current state-of-the-art biobank preservation methods	393:445	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	4	55	theme	tissue	584:589	arg1	samples					591:597	Paired tumor and adjacent normal tissue samples	551:597	samples	591:597	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
29321476	3	56	theme	cancer	535:540	arg1	tissues					542:548	the gastric cancer tissues	523:548	the gastric cancer tissues	523:548	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	10	57	theme	normal	1298:1303	arg1	area					1305:1308	the normal area	1294:1308	the normal area	1294:1308	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	4	58	theme	normal	577:582	arg1	samples					591:597	Paired tumor and adjacent normal tissue samples	551:597	samples	591:597	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
29321476	11	59	theme	cancer	1549:1554	arg1	prognosis					1528:1536	prognosis	1528:1536	prognosis	1528:1536	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	11	59	theme	cancer	1549:1554	arg1	detection					1514:1522	detection	1514:1522	detection	1514:1522	The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.					
29321476	10	60	theme	cancer	1255:1260	arg1	area					1262:1265	the cancer area	1251:1265	the cancer area	1251:1265	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	10	61	dep	epitope	1346:1352	arg1	N'-diacetyllactosamine					1357:1378	N'-diacetyllactosamine	1357:1378	N'-diacetyllactosamine	1357:1378	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	10	61	dep	epitope	1346:1352	arg1	N					1355:1355	N	1355:1355	N	1355:1355	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	1	62	theme	specimens	222:230	arg1	preservation					206:217	the highest preservation	194:217	the highest preservation of specimens	194:230	Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens.					
29321476	0	63	theme	paired	62:67	arg1	cancer					77:82	paired gastric cancer	62:82	paired gastric cancer	62:82	Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.					
29321476	10	64	theme	cancer	1412:1417	arg1	samples					1419:1425	cancer samples	1412:1425	cancer samples	1412:1425	In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N'-diacetyllactosamine) was significantly decreased in cancer samples.					
29321476	6	65	theme	extracted	785:793	arg1	glycoproteins					795:807	extracted glycoproteins	785:807	extracted glycoproteins	785:807	Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS.					
29321476	6	66	gly	glycoproteins	795:807	arg1	glycoproteins					795:807	extracted glycoproteins	785:807	extracted glycoproteins	785:807	Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS.					
29321476	3	67	theme	biobank	418:424	arg1	methods					439:445	current state-of-the-art biobank preservation methods	393:445	current state-of-the-art biobank preservation methods	393:445	Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues.					
29321476	4	68	theme	cancer	650:655	arg1	surgery					657:663	gastric cancer surgery	642:663	gastric cancer surgery	642:663	Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery.					
30733536	8	0	theme	glycosidic	1098:1107	arg1	patterns					1117:1124	partial glycosidic linkage patterns	1090:1124	partial glycosidic linkage patterns	1090:1124	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	4	1	theme	tiny	463:466	arg1	organ					468:472	a tiny organ	461:472	a tiny organ mediating hearing	461:490	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	4	1	theme	tiny	463:466	arg1	ear					456:458	the mammalian inner ear	436:458	the mammalian inner ear	436:458	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	8	2	theme	other	1072:1076	arg1	species					1081:1087	the other 24 species	1068:1087	the other 24 species	1068:1087	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	0	3	theme	cochlea	71:77	arg1	tissue					53:58	the epithelial-like tissue	33:58	the epithelial-like tissue of the rat cochlea	33:77	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.					
30733536	9	4	theme	several	1231:1237	arg1	glycans					1250:1256	several sialylated glycans	1231:1256	several sialylated glycans	1231:1256	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	6	5	theme	electrochemical	776:790	arg1	balance					792:798	the electrochemical balance	772:798	the electrochemical balance in the cochlea	772:813	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	1	6	dep	proteins	89:96	arg1	such					99:102	such	99:102	such	99:102	Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities.					
30733536	7	7	link	N-linked	934:941	arg1	glycans					943:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans were identified in the rat stria vascularis.					
30733536	0	8	from	Characterisation	0:15	arg1	tissue					53:58	the epithelial-like tissue	33:58	the epithelial-like tissue of the rat cochlea	33:77	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.					
30733536	5	9	theme	stria	541:545	arg1	tissue					578:583	an epithelial-like tissue	559:583	an epithelial-like tissue in the cochlea	559:598	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	5	9	theme	stria	541:545	arg1	vascularis					547:556	the stria vascularis	537:556	the stria vascularis	537:556	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	8	10	theme	linkage	1109:1115	arg1	patterns					1117:1124	partial glycosidic linkage patterns	1090:1124	partial glycosidic linkage patterns	1090:1124	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	4	11	theme	ear	456:458	arg1	cochlea					425:431	the cochlea	421:431	the cochlea of the mammalian inner ear, a tiny organ mediating hearing,	421:491	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	7	12	theme	Seventy-nine	921:932	arg1	glycans					943:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans were identified in the rat stria vascularis.					
30733536	3	13	theme	several	321:327	arg1	organs					329:334	several organs	321:334	several organs	321:334	In several organs, profiles of N-glycans have been determined by comprehensive analyses.					
30733536	4	14	theme	inner	450:454	arg1	organ					468:472	a tiny organ	461:472	a tiny organ mediating hearing	461:490	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	4	14	theme	inner	450:454	arg1	ear					456:458	the mammalian inner ear	436:458	the mammalian inner ear	436:458	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	10	15	theme	molecular	1488:1496	arg1	architecture					1498:1509	the molecular architecture	1484:1509	the molecular architecture of the cochlea	1484:1524	These data should accelerate elucidation of the molecular architecture of the cochlea.					
30733536	7	16	theme	N-linked	934:941	arg1	glycans					943:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans	921:949	Seventy-nine N-linked glycans were identified in the rat stria vascularis.					
30733536	9	17	theme	different	1293:1301	arg1	reactions					1318:1326	two different alkylamidation reactions	1289:1326	two different alkylamidation reactions	1289:1326	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	0	18	theme	epithelial-like	37:51	arg1	tissue					53:58	the epithelial-like tissue	33:58	the epithelial-like tissue of the rat cochlea	33:77	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.					
30733536	7	19	theme	rat	974:976	arg1	vascularis					984:993	the rat stria vascularis	970:993	the rat stria vascularis	970:993	Seventy-nine N-linked glycans were identified in the rat stria vascularis.					
30733536	8	20	theme	full	1130:1133	arg1	profiles					1135:1142	full profiles	1130:1142	full profiles of the monosaccharide composition	1130:1176	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	8	21	theme	partial	1090:1096	arg1	patterns					1117:1124	partial glycosidic linkage patterns	1090:1124	partial glycosidic linkage patterns	1090:1124	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	8	22	theme	complete	1028:1035	arg1	structures					1037:1046	the complete structures	1024:1046	the complete structures	1024:1046	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	1	23	theme	Membrane	80:87	arg1	proteins					89:96	Membrane proteins	80:96	Membrane proteins (such as ion channels, transporters, and receptors)	80:148	Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities.					
30733536	1	24	theme	secreted	154:161	arg1	proteins					163:170	secreted proteins	154:170	secreted proteins	154:170	Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities.					
30733536	5	25	from	tissue	578:583	arg1	cochlea					592:598	the cochlea	588:598	the cochlea	588:598	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	8	26	theme	monosaccharide	1151:1164	arg1	composition					1166:1176	the monosaccharide composition	1147:1176	the monosaccharide composition	1147:1176	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	10	27	theme	cochlea	1518:1524	arg1	architecture					1498:1509	the molecular architecture	1484:1509	the molecular architecture of the cochlea	1484:1524	These data should accelerate elucidation of the molecular architecture of the cochlea.					
30733536	6	28	theme	ion	731:733	arg1	transporters					735:746	several ion transporters	723:746	several ion transporters	723:746	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	3	29	theme	N-glycans	349:357	arg1	profiles					337:344	profiles	337:344	profiles of N-glycans	337:357	In several organs, profiles of N-glycans have been determined by comprehensive analyses.					
30733536	6	30	theme	different	833:841	arg1	transporters					843:854	different transporters	833:854	different transporters	833:854	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	2	31	theme	proteins	281:288	arg1	function					263:270	function	263:270	function	263:270	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	2	31	theme	proteins	281:288	arg1	stability					249:257	stability	249:257	stability	249:257	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	3	32	theme	comprehensive	383:395	arg1	analyses					397:404	comprehensive analyses	383:404	comprehensive analyses	383:404	In several organs, profiles of N-glycans have been determined by comprehensive analyses.					
30733536	5	33	theme	mass	659:662	arg1	spectrometry					664:675	mass spectrometry	659:675	mass spectrometry	659:675	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	9	34	gly	sialylated	1239:1248	arg1	glycans					1250:1256	several sialylated glycans	1231:1256	several sialylated glycans	1231:1256	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	5	35	with	chromatography	639:652	arg1	spectrometry					664:675	mass spectrometry	659:675	mass spectrometry	659:675	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	2	36	gly	glycosylation	220:232	arg1	function					263:270	function	263:270	function	263:270	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	2	36	gly	glycosylation	220:232	arg2	residues					308:315	Asn residues	304:315	Asn residues	304:315	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	2	36	gly	glycosylation	220:232	arg1	stability					249:257	stability	249:257	stability	249:257	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	6	37	from	balance	792:798	arg1	cochlea					807:813	the cochlea	803:813	the cochlea	803:813	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	4	38	theme	mammalian	440:448	arg1	organ					468:472	a tiny organ	461:472	a tiny organ mediating hearing	461:490	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	4	38	theme	mammalian	440:448	arg1	ear					456:458	the mammalian inner ear	436:458	the mammalian inner ear	436:458	Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined.					
30733536	2	39	theme	N-linked	211:218	arg1	glycosylation					220:232	N-linked glycosylation	211:232	N-linked glycosylation	211:232	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	6	40	theme	several	723:729	arg1	transporters					735:746	several ion transporters	723:746	several ion transporters	723:746	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	1	41	theme	cellular	190:197	arg1	activities					199:208	cellular activities	190:208	cellular activities	190:208	Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities.					
30733536	6	42	theme	organ	914:918	arg1	structure					884:892	structure	884:892	structure	884:892	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	6	42	theme	organ	914:918	arg1	activity					898:905	activity	898:905	activity	898:905	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	8	43	theme	composition	1166:1176	arg1	patterns					1117:1124	partial glycosidic linkage patterns	1090:1124	partial glycosidic linkage patterns	1090:1124	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	8	43	theme	composition	1166:1176	arg1	profiles					1135:1142	full profiles	1130:1142	full profiles of the monosaccharide composition	1130:1176	Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified.					
30733536	10	44	theme	architecture	1498:1509	arg1	elucidation					1469:1479	elucidation	1469:1479	elucidation of the molecular architecture of the cochlea	1469:1524	These data should accelerate elucidation of the molecular architecture of the cochlea.					
30733536	9	45	theme	characterisation	1213:1228	arg1	process					1202:1208	the process	1198:1208	the process of characterisation	1198:1228	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	5	46	theme	epithelial-like	562:576	arg1	tissue					578:583	an epithelial-like tissue	559:583	an epithelial-like tissue in the cochlea	559:598	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	5	46	theme	epithelial-like	562:576	arg1	vascularis					547:556	the stria vascularis	537:556	the stria vascularis	537:556	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	7	47	theme	stria	978:982	arg1	vascularis					984:993	the rat stria vascularis	970:993	the rat stria vascularis	970:993	Seventy-nine N-linked glycans were identified in the rat stria vascularis.					
30733536	1	48	theme	ion	107:109	arg1	channels					111:118	ion channels	107:118	ion channels	107:118	Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities.					
30733536	9	49	theme	α2,3-linkage	1371:1382	arg1	isomers					1408:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	5	50	theme	liquid	632:637	arg1	chromatography					639:652	liquid chromatography	632:652	liquid chromatography with mass spectrometry	632:675	Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry.					
30733536	9	51	theme	mass	1421:1424	arg1	spectrometry					1426:1437	mass spectrometry	1421:1437	mass spectrometry	1421:1437	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	2	52	theme	Asn	304:306	arg1	residues					308:315	Asn residues	304:315	Asn residues	304:315	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
30733536	9	53	theme	α2,6-linkage	1388:1399	arg1	isomers					1408:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	0	54	theme	rat	67:69	arg1	cochlea					71:77	the rat cochlea	63:77	the rat cochlea	63:77	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.					
30733536	9	55	theme	sialylated	1239:1248	arg1	glycans					1250:1256	several sialylated glycans	1231:1256	several sialylated glycans	1231:1256	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	6	56	theme	hypervascular	683:695	arg1	tissue					697:702	This hypervascular tissue	678:702	This hypervascular tissue	678:702	This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ.					
30733536	9	57	theme	sialyl	1401:1406	arg1	isomers					1408:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	α2,3-linkage and α2,6-linkage sialyl isomers	1371:1414	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	0	58	theme	N-glycans	20:28	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.	0:78	Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea.					
30733536	9	59	theme	alkylamidation	1303:1316	arg1	reactions					1318:1326	two different alkylamidation reactions	1289:1326	two different alkylamidation reactions	1289:1326	In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry.					
30733536	2	60	link	N-linked	211:218	arg1	glycosylation					220:232	N-linked glycosylation	211:232	N-linked glycosylation	211:232	N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues.					
31737090	8	0	theme	domain	1994:1999	arg1	configuration					2001:2013	the β-sandwich domain configuration	1979:2013	the β-sandwich domain configuration	1979:2013	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	2	1	theme	recombinant	364:374	arg1	exo-inulinase					376:388	a recombinant exo-inulinase	362:388	a recombinant exo-inulinase (rKcINU1)	362:398	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	1	theme	recombinant	364:374	arg1	rKcINU1					391:397	rKcINU1	391:397	rKcINU1	391:397	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	1	theme	recombinant	364:374	arg1	glycoprotein					476:487	an N-linked glycoprotein	464:487	an N-linked glycoprotein	464:487	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	6	2	theme	various	1426:1432	arg1	extents					1434:1440	various extents	1426:1440	various extents	1426:1440	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	1	3	theme	medical	279:285	arg1	industry					287:294	the medical industry	275:294	the medical industry	275:294	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	8	4	dep	fine-tuning	1967:1977	arg1	means					1958:1962	means	1958:1962	means	1958:1962	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	7	5	theme	hindrance	1767:1775	arg1	chains					1686:1691	the N-linked glycan chains	1666:1691	the N-linked glycan chains at the Asn-362 position	1666:1715	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	5	theme	hindrance	1767:1775	arg1	type					1752:1755	a type	1750:1755	a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1750:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	4	6	located	located	807:813	arg2	Asn-526					793:799	Asn-526	793:799	Asn-526	793:799	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	6	located	located	807:813	arg2	sites					747:751	all five glycosylation sites	724:751	all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526)	724:800	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	6	located	located	807:813	arg2	Asn-467					781:787	Asn-467	781:787	Asn-467	781:787	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	6	located	located	807:813	arg1	conducive					885:893	conducive	885:893	conducive	885:893	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	6	located	located	807:813	arg1	domain					844:849	the C-terminus β-sandwich domain	818:849	the C-terminus β-sandwich domain	818:849	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	10	7	theme	amino	2442:2446	arg1	sequence					2453:2460	the amino acid sequence	2438:2460	the amino acid sequence	2438:2460	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	0	8	theme	Unique	0:5	arg1	N-glycosylation					7:21	Unique N-glycosylation	0:21	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus	0:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	8	9	theme	other	1864:1868	arg1	sites					1875:1879	the other four sites	1860:1879	the other four sites	1860:1879	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	6	10	dep	thermostability	1295:1309	arg1	decrease					1320:1327	2.91 °C decrease	1312:1327	2.91 °C decrease in T	1312:1332	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	4	11	theme	glycosylation	916:928	arg1	modification					930:941	glycosylation modification	916:941	glycosylation modification	916:941	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	3	12	theme	N-glycosylation	570:584	arg1	sites					586:590	Five N-glycosylation sites	565:590	Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	565:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	6	13	theme	lowest	1478:1483	arg1	activity					1492:1499	the lowest enzyme activity	1474:1499	the lowest enzyme activity	1474:1499	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	7	14	theme	kinetics	1605:1612	arg1	data					1614:1617	kinetics data	1605:1617	kinetics data	1605:1617	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	9	15	theme	binding	2160:2166	arg1	modules					2168:2174	carbohydrate binding modules	2147:2174	carbohydrate binding modules (CBMs)	2147:2181	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	9	15	theme	binding	2160:2166	arg1	CBMs					2177:2180	CBMs	2177:2180	CBMs	2177:2180	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	4	16	from	domain	844:849	arg1	located					807:813	located	807:813	located	807:813	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	0	17	from	cicerisporus	73:84	arg1	exo-inulinase					40:52	a recombinant exo-inulinase	26:52	a recombinant exo-inulinase from Kluyveromyces cicerisporus	26:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	17	from	cicerisporus	73:84	arg1	effect					94:99	its effect	90:99	its effect on enzymatic activity and thermostability	90:141	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	17	from	cicerisporus	73:84	arg1	N-glycosylation					7:21	Unique N-glycosylation	0:21	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus	0:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	6	18	theme	%	1211:1211	arg1	increase					1213:1220	an 18% increase	1206:1220	an 18% increase in the specific activity against inulin	1206:1260	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	3	19	theme	high	606:609	arg1	Man3-9GlcNAc2					642:654	Man3-9GlcNAc2	642:654	Man3-9GlcNAc2	642:654	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	3	19	theme	high	606:609	arg1	oligosaccharides					624:639	variable high mannose-type oligosaccharides	597:639	variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	597:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	9	20	with	endowed	2200:2206	arg1	functions					2239:2247	the functions	2235:2247	the functions of CBM	2235:2254	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	3	21	gly	N-glycosylation	570:584	arg2	sites					586:590	Five N-glycosylation sites	565:590	Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	565:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	3	21	gly	N-glycosylation	570:584	arg2	Five					565:568	Five	565:568	Five	565:568	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	6	22	theme	significant	1271:1281	arg1	decrease					1283:1290	a significant decrease	1269:1290	a significant decrease in thermostability (2.91 °C decrease in T )	1269:1334	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	6	23	from	decrease	1389:1396	arg1	activity					1414:1421	the specific activity	1401:1421	the specific activity	1401:1421	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	3	24	dep	RESULTS	557:563	arg1	confirmed					662:670	confirmed	662:670	were confirmed in the rKcINU1	657:685	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	7	25	theme	thermostability	1567:1581	arg1	testing					1583:1589	thermostability testing	1567:1589	thermostability testing	1567:1589	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	2	26	theme	N-linked	509:516	arg1	chains					525:530	N-linked glycan chains	509:530	N-linked glycan chains	509:530	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	6	27	from	increase	1213:1220	arg1	activity					1238:1245	the specific activity	1225:1245	the specific activity against inulin	1225:1260	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	6	28	theme	single	1356:1361	arg1	mutations					1363:1371	other single mutations	1350:1371	other single mutations	1350:1371	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	7	29	theme	glycan	1679:1684	arg1	chains					1686:1691	the N-linked glycan chains	1666:1691	the N-linked glycan chains at the Asn-362 position	1666:1715	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	29	theme	glycan	1679:1684	arg1	type					1752:1755	a type	1750:1755	a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1750:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	4	30	theme	N-terminus	952:961	arg1	domain					963:968	the N-terminus domain	948:968	the N-terminus domain	948:968	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	5	31	theme	N-glycosylation	983:997	arg1	mutants					999:1005	Single-site N-glycosylation mutants	971:1005	Single-site N-glycosylation mutants with Asn substituted by Gln	971:1033	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	2	32	theme	chains	525:530	arg1	removal					498:504	the removal	494:504	the removal of N-linked glycan chains	494:530	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	1	33	theme	Jerusalem	319:327	arg1	artichoke					329:337	Jerusalem artichoke	319:337	Jerusalem artichoke	319:337	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	2	34	link	N-linked	467:474	arg1	glycoprotein					476:487	an N-linked glycoprotein	464:487	an N-linked glycoprotein	464:487	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	34	link	N-linked	467:474	arg1	exo-inulinase					376:388	a recombinant exo-inulinase	362:388	a recombinant exo-inulinase (rKcINU1)	362:398	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	5	35	with	Mut	1058:1060	arg1	sites					1092:1096	all five N-glycosylation sites	1067:1096	all five N-glycosylation sites removed	1067:1104	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	6	36	theme	specific	1405:1412	arg1	activity					1414:1421	the specific activity	1401:1421	the specific activity	1401:1421	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	7	37	from	activity	1534:1541	arg1	N362Q					1546:1550	N362Q	1546:1550	N362Q	1546:1550	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	38	theme	secondary	1623:1631	arg1	analysis					1643:1650	secondary structure analysis	1623:1650	secondary structure analysis	1623:1650	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	0	39	theme	recombinant	28:38	arg1	exo-inulinase					40:52	a recombinant exo-inulinase	26:52	a recombinant exo-inulinase from Kluyveromyces cicerisporus	26:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	6	40	from	decrease	1283:1290	arg1	thermostability					1295:1309	thermostability	1295:1309	thermostability (2.91 °C decrease in T )	1295:1334	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	0	41	gly	N-glycosylation	7:21	arg1	exo-inulinase					40:52	a recombinant exo-inulinase	26:52	a recombinant exo-inulinase from Kluyveromyces cicerisporus	26:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	41	gly	N-glycosylation	7:21	arg1	thermostability					127:141	thermostability	127:141	thermostability	127:141	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	41	gly	N-glycosylation	7:21	arg1	activity					114:121	enzymatic activity	104:121	enzymatic activity	104:121	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	10	42	theme	enzymes	2403:2409	arg1	view					2374:2377	a novel view	2366:2377	a novel view for glycoengineering of enzymes from fungi	2366:2420	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	1	43	theme	artichoke	329:337	arg1	food					269:272	food	269:272	food	269:272	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	1	43	theme	artichoke	329:337	arg1	industry					287:294	the medical industry	275:294	the medical industry	275:294	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	1	43	theme	artichoke	329:337	arg1	biorefinery					304:314	the biorefinery	300:314	the biorefinery of Jerusalem artichoke	300:337	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	8	44	theme	substrate	1936:1944	arg1	affinity					1946:1953	altered substrate affinity	1928:1953	altered substrate affinity	1928:1953	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	5	45	theme	enzyme	1157:1162	arg1	activity					1164:1171	all enzyme activity	1153:1171	all enzyme activity	1153:1171	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	2	46	attach	derived	401:407	arg2	rKcINU1					391:397	rKcINU1	391:397	rKcINU1	391:397	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	46	attach	derived	401:407	arg2	exo-inulinase					376:388	a recombinant exo-inulinase	362:388	a recombinant exo-inulinase (rKcINU1)	362:398	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	46	attach	derived	401:407	arg1	CBS4857					441:447	Kluyveromyces cicerisporus CBS4857	414:447	Kluyveromyces cicerisporus CBS4857	414:447	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	46	attach	derived	401:407	arg2	glycoprotein					476:487	an N-linked glycoprotein	464:487	an N-linked glycoprotein	464:487	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	47	gly	glycoprotein	476:487	arg1	glycoprotein					476:487	an N-linked glycoprotein	464:487	an N-linked glycoprotein	464:487	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	47	gly	glycoprotein	476:487	arg1	exo-inulinase					376:388	a recombinant exo-inulinase	362:388	a recombinant exo-inulinase (rKcINU1)	362:398	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	5	48	gly	N-glycosylation	1076:1090	arg2	sites					1092:1096	all five N-glycosylation sites	1067:1096	all five N-glycosylation sites removed	1067:1104	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	5	48	gly	N-glycosylation	1076:1090	arg2	five					1071:1074	five	1071:1074	five	1071:1074	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	9	49	dep	chains	2193:2198	arg1	i.e.					2184:2187	i.e.	2184:2187	i.e.	2184:2187	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	9	49	dep	chains	2193:2198	arg1	endowed					2200:2206	endowed	2200:2206	endowed	2200:2206	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	7	50	theme	adjacent	1788:1795	arg1	N-glycans					1797:1805	its adjacent N-glycans to bring rigidity	1784:1823	its adjacent N-glycans to bring rigidity	1784:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	9	51	theme	enzyme	2088:2093	arg1	cavity					2102:2107	the enzyme active cavity	2084:2107	the enzyme active cavity	2084:2107	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	8	52	from	sites	1875:1879	arg1	Meanwhile					1826:1834	Meanwhile	1826:1834	Meanwhile	1826:1834	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	8	52	from	sites	1875:1879	arg1	N-glycosylation					1841:1855	the N-glycosylation	1837:1855	the N-glycosylation at the other four sites	1837:1879	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	7	53	theme	steric	1760:1765	arg1	hindrance					1767:1775	steric hindrance	1760:1775	steric hindrance toward its adjacent N-glycans to bring rigidity	1760:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	1	54	theme	high-fructose	196:208	arg1	syrups					210:215	high-fructose syrups	196:215	high-fructose syrups	196:215	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	2	55	theme	N-linked	467:474	arg1	glycoprotein					476:487	an N-linked glycoprotein	464:487	an N-linked glycoprotein	464:487	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	55	theme	N-linked	467:474	arg1	exo-inulinase					376:388	a recombinant exo-inulinase	362:388	a recombinant exo-inulinase (rKcINU1)	362:398	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	9	56	theme	similar	2128:2134	arg1	manner					2115:2120	a manner	2113:2120	a manner quite similar to that of carbohydrate binding modules (CBMs)	2113:2181	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	6	57	theme	specific	1229:1236	arg1	activity					1238:1245	the specific activity	1225:1245	the specific activity against inulin	1225:1260	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	7	58	from	position	1708:1715	arg1	chains					1686:1691	the N-linked glycan chains	1666:1691	the N-linked glycan chains at the Asn-362 position	1666:1715	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	58	from	position	1708:1715	arg1	type					1752:1755	a type	1750:1755	a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1750:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	4	59	theme	structural	692:701	arg1	modeling					703:710	The structural modeling	688:710	The structural modeling	688:710	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	domain					844:849	the C-terminus β-sandwich domain	818:849	the C-terminus β-sandwich domain	818:849	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	conducive					885:893	conducive	885:893	conducive	885:893	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	five					728:731	five	728:731	five	728:731	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	sites					747:751	all five glycosylation sites	724:751	all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526)	724:800	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	Asn-467					781:787	Asn-467	781:787	Asn-467	781:787	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	60	gly	glycosylation	733:745	arg2	Asn-526					793:799	Asn-526	793:799	Asn-526	793:799	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	6	61	theme	enzyme	1485:1490	arg1	activity					1492:1499	the lowest enzyme activity	1474:1499	the lowest enzyme activity	1474:1499	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	0	62	from	effect	94:99	arg1	cicerisporus					73:84	Kluyveromyces cicerisporus	59:84	Kluyveromyces cicerisporus	59:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	62	from	effect	94:99	arg1	activity					114:121	enzymatic activity	104:121	enzymatic activity	104:121	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	62	from	effect	94:99	arg1	thermostability					127:141	thermostability	127:141	thermostability	127:141	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	5	63	with	mutants	999:1005	arg1	Asn					1012:1014	Asn	1012:1014	Asn substituted by Gln	1012:1033	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	9	64	theme	carbohydrate	2147:2158	arg1	modules					2168:2174	carbohydrate binding modules	2147:2174	carbohydrate binding modules (CBMs)	2147:2181	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	9	64	theme	carbohydrate	2147:2158	arg1	CBMs					2177:2180	CBMs	2177:2180	CBMs	2177:2180	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	8	65	theme	enzyme	1902:1907	arg1	activity					1909:1916	enzyme activity	1902:1916	enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration	1902:2013	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	5	66	theme	N-glycosylation	1076:1090	arg1	sites					1092:1096	all five N-glycosylation sites	1067:1096	all five N-glycosylation sites removed	1067:1104	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	3	67	theme	mannose-type	611:622	arg1	Man3-9GlcNAc2					642:654	Man3-9GlcNAc2	642:654	Man3-9GlcNAc2	642:654	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	3	67	theme	mannose-type	611:622	arg1	oligosaccharides					624:639	variable high mannose-type oligosaccharides	597:639	variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	597:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	7	68	theme	3D	1592:1593	arg1	modeling					1595:1602	3D modeling	1592:1602	3D modeling	1592:1602	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	4	69	theme	modification	930:941	arg1	occurrence					902:911	the occurrence	898:911	the occurrence of glycosylation modification than the N-terminus domain	898:968	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	10	70	theme	acid	2448:2451	arg1	sequence					2453:2460	the amino acid sequence	2438:2460	the amino acid sequence	2438:2460	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	3	71	theme	variable	597:604	arg1	Man3-9GlcNAc2					642:654	Man3-9GlcNAc2	642:654	Man3-9GlcNAc2	642:654	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	3	71	theme	variable	597:604	arg1	oligosaccharides					624:639	variable high mannose-type oligosaccharides	597:639	variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	597:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	10	72	theme	unique	2281:2286	arg1	sequence					2299:2306	a unique C-terminal sequence	2279:2306	a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties	2279:2529	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	72	theme	unique	2281:2286	arg1	favorable					2322:2330	favorable	2322:2330	favorable	2322:2330	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	6	73	theme	18	1209:1210	arg1	%					1211:1211	%	1211:1211	%	1211:1211	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	2	74	link	N-linked	509:516	arg1	chains					525:530	N-linked glycan chains	509:530	N-linked glycan chains	509:530	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	4	75	theme	glycosylation	733:745	arg1	sites					747:751	all five glycosylation sites	724:751	all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526)	724:800	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	75	theme	glycosylation	733:745	arg1	Asn-526					793:799	Asn-526	793:799	Asn-526	793:799	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	75	theme	glycosylation	733:745	arg1	Asn-467					781:787	Asn-467	781:787	Asn-467	781:787	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	8	76	theme	altered	1928:1934	arg1	affinity					1946:1953	altered substrate affinity	1928:1953	altered substrate affinity	1928:1953	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	1	77	theme	BACKGROUND	144:153	arg1	Inulinase					155:163	BACKGROUND Inulinase	144:163	BACKGROUND Inulinase	144:163	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	10	78	theme	C-terminal	2288:2297	arg1	sequence					2299:2306	a unique C-terminal sequence	2279:2306	a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties	2279:2529	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	78	theme	C-terminal	2288:2297	arg1	favorable					2322:2330	favorable	2322:2330	favorable	2322:2330	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	79	theme	C-terminal	2469:2478	arg1	domain					2480:2485	the C-terminal domain	2465:2485	the C-terminal domain	2465:2485	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	80	from	view	2374:2377	arg1	fungi					2416:2420	fungi	2416:2420	fungi	2416:2420	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	6	81	theme	other	1350:1354	arg1	mutations					1363:1371	other single mutations	1350:1371	other single mutations	1350:1371	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	0	82	from	N-glycosylation	7:21	arg1	cicerisporus					73:84	Kluyveromyces cicerisporus	59:84	Kluyveromyces cicerisporus	59:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	82	from	N-glycosylation	7:21	arg1	activity					114:121	enzymatic activity	104:121	enzymatic activity	104:121	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	82	from	N-glycosylation	7:21	arg1	thermostability					127:141	thermostability	127:141	thermostability	127:141	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	83	theme	enzymatic	104:112	arg1	activity					114:121	enzymatic activity	104:121	enzymatic activity	104:121	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	5	84	theme	Single-site	971:981	arg1	mutants					999:1005	Single-site N-glycosylation mutants	971:1005	Single-site N-glycosylation mutants with Asn substituted by Gln	971:1033	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	7	85	theme	enzyme	1527:1532	arg1	activity					1534:1541	The increased enzyme activity	1513:1541	The increased enzyme activity	1513:1541	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	9	86	theme	β-sandwich	2212:2221	arg1	domain					2223:2228	the β-sandwich domain	2208:2228	the β-sandwich domain	2208:2228	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	8	87	theme	β-sandwich	1983:1992	arg1	configuration					2001:2013	the β-sandwich domain configuration	1979:2013	the β-sandwich domain configuration	1979:2013	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	7	88	theme	N-linked	1670:1677	arg1	chains					1686:1691	the N-linked glycan chains	1666:1691	the N-linked glycan chains at the Asn-362 position	1666:1715	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	88	theme	N-linked	1670:1677	arg1	type					1752:1755	a type	1750:1755	a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1750:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	2	89	theme	glycan	518:523	arg1	chains					525:530	N-linked glycan chains	509:530	N-linked glycan chains	509:530	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	10	90	from	fungi	2416:2420	arg1	enzymes					2403:2409	enzymes	2403:2409	enzymes from fungi	2403:2420	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	90	from	fungi	2416:2420	arg1	view					2374:2377	a novel view	2366:2377	a novel view for glycoengineering of enzymes from fungi	2366:2420	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	10	91	theme	enzymatic	2510:2518	arg1	properties					2520:2529	the enzymatic properties	2506:2529	the enzymatic properties	2506:2529	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	7	92	link	N-linked	1670:1677	arg1	chains					1686:1691	the N-linked glycan chains	1666:1691	the N-linked glycan chains at the Asn-362 position	1666:1715	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	92	link	N-linked	1670:1677	arg1	type					1752:1755	a type	1750:1755	a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1750:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	6	93	theme	2.91 °C	1312:1318	arg1	decrease					1320:1327	2.91 °C decrease	1312:1327	2.91 °C decrease in T	1312:1332	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	3	94	with	sites	586:590	arg1	Man3-9GlcNAc2					642:654	Man3-9GlcNAc2	642:654	Man3-9GlcNAc2	642:654	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	3	94	with	sites	586:590	arg1	oligosaccharides					624:639	variable high mannose-type oligosaccharides	597:639	variable high mannose-type oligosaccharides (Man3-9GlcNAc2)	597:655	RESULTS Five N-glycosylation sites with variable high mannose-type oligosaccharides (Man3-9GlcNAc2) were confirmed in the rKcINU1.					
31737090	4	95	dep	sites	747:751	arg1	Asn-467					781:787	Asn-467	781:787	Asn-467	781:787	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	95	dep	sites	747:751	arg1	sites					747:751	all five glycosylation sites	724:751	all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526)	724:800	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	95	dep	sites	747:751	arg1	Asn-526					793:799	Asn-526	793:799	Asn-526	793:799	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	10	96	theme	novel	2368:2372	arg1	view					2374:2377	a novel view	2366:2377	a novel view for glycoengineering of enzymes from fungi	2366:2420	This study discovered a unique C-terminal sequence which is more favorable to glycosylation, thereby casting a novel view for glycoengineering of enzymes from fungi via redesigning the amino acid sequence at the C-terminal domain, so as to optimize the enzymatic properties.					
31737090	9	97	theme	CBM	2252:2254	arg1	functions					2239:2247	the functions	2235:2247	the functions of CBM	2235:2254	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	0	98	theme	exo-inulinase	40:52	arg1	effect					94:99	its effect	90:99	its effect on enzymatic activity and thermostability	90:141	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	0	98	theme	exo-inulinase	40:52	arg1	N-glycosylation					7:21	Unique N-glycosylation	0:21	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus	0:84	Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability.					
31737090	6	99	from	decrease	1320:1327	arg1	T					1332:1332	T	1332:1332	T	1332:1332	Interestingly, the N362Q led to an 18% increase in the specific activity against inulin, while a significant decrease in thermostability (2.91 °C decrease in T ) occurred, and other single mutations resulted in the decrease in the specific activity to various extents, among which N467Q demonstrated the lowest enzyme activity.					
31737090	7	100	theme	increased	1517:1525	arg1	activity					1534:1541	The increased enzyme activity	1513:1541	The increased enzyme activity	1513:1541	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	7	101	theme	structure	1633:1641	arg1	analysis					1643:1650	secondary structure analysis	1623:1650	secondary structure analysis	1623:1650	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	2	102	dep	Kluyveromyces	414:426	arg1	cicerisporus					428:439	cicerisporus	428:439	cicerisporus	428:439	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	103	theme	Kluyveromyces	414:426	arg1	CBS4857					441:447	Kluyveromyces cicerisporus CBS4857	414:447	Kluyveromyces cicerisporus CBS4857	414:447	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	2	104	theme	reduced	539:545	arg1	activity					547:554	reduced activity	539:554	reduced activity	539:554	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	9	105	theme	substrates	2070:2079	arg1	transfer					2058:2065	transfer	2058:2065	transfer	2058:2065	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	9	105	theme	substrates	2070:2079	arg1	capture					2046:2052	the capture	2042:2052	the capture	2042:2052	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31737090	4	106	theme	β-sandwich	833:842	arg1	conducive					885:893	conducive	885:893	conducive	885:893	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	4	106	theme	β-sandwich	833:842	arg1	domain					844:849	the C-terminus β-sandwich domain	818:849	the C-terminus β-sandwich domain	818:849	The structural modeling showed that all five glycosylation sites (Asn-362, Asn-370, Asn-399, Asn-467 and Asn-526) were located at the C-terminus β-sandwich domain, which has been proven to be more conducive to the occurrence of glycosylation modification than the N-terminus domain.					
31737090	5	107	theme	activity	1164:1171	arg1	loss					1145:1148	the loss	1141:1148	the loss of all enzyme activity	1141:1171	Single-site N-glycosylation mutants with Asn substituted by Gln were obtained, and the Mut with all five N-glycosylation sites removed was constructed, which resulted in the loss of all enzyme activity.					
31737090	2	108	theme	present	347:353	arg1	study					355:359	the present study	343:359	the present study	343:359	In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as an N-linked glycoprotein, and the removal of N-linked glycan chains led to reduced activity.					
31737090	7	109	dep	CONCLUSION	1502:1511	arg1	implied					1653:1659	implied	1653:1659	implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity	1653:1823	CONCLUSION The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the N-linked glycan chains at the Asn-362 position functioned negatively, mainly as a type of steric hindrance toward its adjacent N-glycans to bring rigidity.					
31737090	1	110	used	used	261:264	arg2	fructoligosaccharides					221:241	fructoligosaccharides	221:241	fructoligosaccharides	221:241	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	1	110	used	used	261:264	arg2	syrups					210:215	high-fructose syrups	196:215	high-fructose syrups	196:215	BACKGROUND Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke.					
31737090	8	111	gly	N-glycosylation	1841:1855	arg2	sites					1875:1879	the other four sites	1860:1879	the other four sites	1860:1879	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	8	111	gly	N-glycosylation	1841:1855	arg1	sites					1875:1879	the other four sites	1860:1879	the other four sites	1860:1879	Meanwhile, the N-glycosylation at the other four sites positively regulated enzyme activity caused by altered substrate affinity by means of fine-tuning the β-sandwich domain configuration.					
31737090	9	112	theme	active	2095:2100	arg1	cavity					2102:2107	the enzyme active cavity	2084:2107	the enzyme active cavity	2084:2107	This may have facilitated the capture and transfer of substrates to the enzyme active cavity, in a manner quite similar to that of carbohydrate binding modules (CBMs), i.e. the chains endowed the β-sandwich domain with the functions of CBM.					
31325506	4	0	dep	Fucose0~1Hexose3~4N-acetylhexosamine2~3	1110:1148	arg1	%					1155:1155	26.9%	1151:1155	Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%	1110:1155	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	5	1	theme	less-branched	1254:1266	arg1	structures					1268:1277	less-branched structures	1254:1277	less-branched structures	1254:1277	Additionally, these are less-branched structures compared to human mucins.					
31325506	5	1	theme	less-branched	1254:1266	arg1	these					1244:1248	these	1244:1248	these	1244:1248	Additionally, these are less-branched structures compared to human mucins.					
31325506	3	2	theme	liquid	784:789	arg1	spectrometry					818:829	liquid chromatography-tandem mass spectrometry	784:829	liquid chromatography-tandem mass spectrometry	784:829	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	4	3	dep	[Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1	880:929	arg1	%					936:936	61.1%	932:936	61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)	932:1017	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	4	4	dep	N-glycans	1008:1016	arg1	out					991:993	out	991:993	out	991:993	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	1	5	theme	heavily-glycosylated	150:169	arg1	protein					208:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	5	theme	heavily-glycosylated	150:169	arg1	mucin					133:137	Bovine submaxillary mucin	113:137	Bovine submaxillary mucin (BSM)	113:143	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	6	6	theme	unique	1411:1416	arg1	functions					1429:1437	unique biological functions	1411:1437	unique biological functions	1411:1437	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	3	7	theme	chromatography-tandem	791:811	arg1	spectrometry					818:829	liquid chromatography-tandem mass spectrometry	784:829	liquid chromatography-tandem mass spectrometry	784:829	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	4	8	theme	relative	954:961	arg1	quantities					963:972	the relative quantities	950:972	the relative quantities of each N-glycan	950:989	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	4	9	theme	sialylated	1197:1206	arg1	N-glycans					1208:1216	no hybrid-type or sialylated N-glycans	1179:1216	no hybrid-type or sialylated N-glycans	1179:1216	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	4	10	theme	complex-type	867:878	arg1	[Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1					880:929	ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1	863:929	ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)]	863:1018	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	6	11	gly	fucosylated	1385:1395	arg1	glycans					1359:1365	two sulfated glycans	1346:1365	two sulfated glycans (8.0%)	1346:1372	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	6	11	gly	fucosylated	1385:1395	arg1	glycans					1319:1325	ten glycans	1315:1325	ten glycans (77.2%)	1315:1333	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	6	11	gly	fucosylated	1385:1395	arg1	%					1332:1332	77.2%	1328:1332	77.2%	1328:1332	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	6	12	theme	biological	1418:1427	arg1	functions					1429:1437	unique biological functions	1411:1437	unique biological functions	1411:1437	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	0	13	theme	sialylated	93:102	arg1	glycans					104:110	sialylated glycans	93:110	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	1	14	theme	biochemical	341:351	arg1	substrate					353:361	a biochemical substrate	339:361	a biochemical substrate	339:361	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	4	15	dep	type	1104:1107	arg1	Fucose0~1Hexose3~4N-acetylhexosamine2~3					1110:1148	Fucose0~1Hexose3~4N-acetylhexosamine2~3	1110:1148	Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%	1110:1155	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	4	16	dep	Hexose5~6N-acetylhexosamine2	1044:1071	arg1	%					1078:1078	12.0%	1074:1078	Hexose5~6N-acetylhexosamine2; 12.0%	1044:1078	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	4	17	theme	N-glycans	1008:1016	arg1	sum					943:945	the sum	939:945	the sum of the relative quantities of each N-glycan out of the total N-glycans	939:1016	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	2	18	theme	most	502:505	arg1	research					511:518	most BSM research	502:518	most BSM research	502:518	Although it has been reported that N-glycosylation provides stability of human mucins, most BSM research has been focused on its O-glycans, while N-glycans have not been reported to date.					
31325506	3	19	theme	BSM	694:696	arg1	analysis					682:689	monosaccharide analysis	667:689	monosaccharide analysis of BSM	667:696	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	4	20	dep	high-mannose-type	1025:1041	arg1	Hexose5~6N-acetylhexosamine2					1044:1071	Hexose5~6N-acetylhexosamine2	1044:1071	Hexose5~6N-acetylhexosamine2; 12.0%	1044:1078	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	0	21	contain	contain	39:45	arg1	N-glycans					0:8	N-glycans	0:8	N-glycans of bovine submaxillary mucin	0:37	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	0	21	contain	contain	39:45	arg2	glycans					104:110	sialylated glycans	93:110	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	0	21	contain	contain	39:45	arg2	glycans					77:83	core-fucosylated and sulfated glycans	47:83	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	4	22	theme	quantities	963:972	arg1	sum					943:945	the sum	939:945	the sum of the relative quantities of each N-glycan out of the total N-glycans	939:1016	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	2	23	theme	BSM	507:509	arg1	research					511:518	most BSM research	502:518	most BSM research	502:518	Although it has been reported that N-glycosylation provides stability of human mucins, most BSM research has been focused on its O-glycans, while N-glycans have not been reported to date.					
31325506	4	24	gly	sialylated	1197:1206	arg1	N-glycans					1208:1216	no hybrid-type or sialylated N-glycans	1179:1216	no hybrid-type or sialylated N-glycans	1179:1216	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	8	25	theme	first	1562:1566	arg1	study					1549:1553	This study	1544:1553	This study	1544:1553	This study is the first confirmation of N-glycan attachment to BSM.					
31325506	8	25	theme	first	1562:1566	arg1	confirmation					1568:1579	the first confirmation	1558:1579	the first confirmation of N-glycan attachment to BSM	1558:1609	This study is the first confirmation of N-glycan attachment to BSM.					
31325506	7	26	theme	N-glycosylation	1461:1475	arg1	sites					1477:1481	The N-glycosylation sites	1457:1481	The N-glycosylation sites	1457:1481	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	7	27	from	analysis	1508:1515	arg1	BSM					1539:1541	BSM	1539:1541	BSM	1539:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	7	28	theme	glycopeptides	1520:1532	arg1	analysis					1508:1515	the analysis	1504:1515	the analysis of glycopeptides from BSM	1504:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	6	29	gly	glycoproteins	1442:1454	arg1	glycoproteins					1442:1454	glycoproteins	1442:1454	glycoproteins	1442:1454	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	4	30	theme	N-glycan	982:989	arg1	quantities					963:972	the relative quantities	950:972	the relative quantities of each N-glycan	950:989	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	0	31	gly	sialylated	93:102	arg1	glycans					104:110	sialylated glycans	93:110	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	7	32	from	BSM	1539:1541	arg1	glycopeptides					1520:1532	glycopeptides	1520:1532	glycopeptides from BSM	1520:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	7	32	from	BSM	1539:1541	arg1	analysis					1508:1515	the analysis	1504:1515	the analysis of glycopeptides from BSM	1504:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	3	33	theme	common	620:625	arg1	component					641:649	a common N-glycan core component	618:649	a common N-glycan core component	618:649	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	1	34	dep	heavily-glycosylated	150:169	arg1	macromolecular					171:184	macromolecular	171:184	macromolecular	171:184	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	4	35	theme	paucimannose	1091:1102	arg1	type					1104:1107	five paucimannose type	1086:1107	five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%)	1086:1156	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	0	36	theme	submaxillary	20:31	arg1	mucin					33:37	bovine submaxillary mucin	13:37	bovine submaxillary mucin	13:37	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	1	37	theme	high	280:283	arg1	viscosity					285:293	its high viscosity	276:293	its high viscosity	276:293	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	7	38	gly	glycopeptides	1520:1532	arg1	BSM					1539:1541	BSM	1539:1541	BSM	1539:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	7	38	gly	glycopeptides	1520:1532	arg2	glycopeptides					1520:1532	glycopeptides	1520:1532	glycopeptides from BSM	1520:1541	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	6	39	theme	sulfated	1350:1357	arg1	glycans					1359:1365	two sulfated glycans	1346:1365	two sulfated glycans (8.0%)	1346:1372	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	6	39	theme	sulfated	1350:1357	arg1	%					1371:1371	8.0%	1368:1371	8.0%	1368:1371	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	0	40	theme	bovine	13:18	arg1	mucin					33:37	bovine submaxillary mucin	13:37	bovine submaxillary mucin	13:37	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	1	41	theme	viscosity	285:293	arg1	light					267:271	light	267:271	light of its high viscosity and biocompatibility	267:314	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	42	theme	Bovine	113:118	arg1	protein					208:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	42	theme	Bovine	113:118	arg1	BSM					140:142	BSM	140:142	BSM	140:142	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	42	theme	Bovine	113:118	arg1	mucin					133:137	Bovine submaxillary mucin	113:137	Bovine submaxillary mucin (BSM)	113:143	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	6	43	theme	these	1308:1312	arg1	fucosylated					1385:1395	core fucosylated	1380:1395	core fucosylated	1380:1395	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	4	44	theme	total	1002:1006	arg1	N-glycans					1008:1016	the total N-glycans	998:1016	the total N-glycans	998:1016	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	1	45	dep	use	332:334	arg1	addition					320:327	addition	320:327	addition	320:327	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	46	from	applications	251:262	arg1	light					267:271	light	267:271	light of its high viscosity and biocompatibility	267:314	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	0	47	theme	mucin	33:37	arg1	N-glycans					0:8	N-glycans	0:8	N-glycans of bovine submaxillary mucin	0:37	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	1	48	theme	biocompatibility	299:314	arg1	light					267:271	light	267:271	light of its high viscosity and biocompatibility	267:314	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	8	49	theme	attachment	1593:1602	arg1	study					1549:1553	This study	1544:1553	This study	1544:1553	This study is the first confirmation of N-glycan attachment to BSM.					
31325506	8	49	theme	attachment	1593:1602	arg1	confirmation					1568:1579	the first confirmation	1558:1579	the first confirmation of N-glycan attachment to BSM	1558:1609	This study is the first confirmation of N-glycan attachment to BSM.					
31325506	3	50	located	detected	655:662	arg1	study					611:615	this study	606:615	this study	606:615	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	3	50	located	detected	655:662	arg2	component					641:649	a common N-glycan core component	618:649	a common N-glycan core component	618:649	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	8	51	theme	N-glycan	1584:1591	arg1	attachment					1593:1602	N-glycan attachment	1584:1602	N-glycan attachment to BSM	1584:1609	This study is the first confirmation of N-glycan attachment to BSM.					
31325506	3	52	theme	N-glycans	725:733	arg1	quantities					754:763	their relative quantities	739:763	their relative quantities	739:763	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	3	52	theme	N-glycans	725:733	arg1	structures					707:716	the structures	703:716	the structures of the N-glycans	703:733	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	2	53	theme	human	488:492	arg1	mucins					494:499	human mucins	488:499	human mucins	488:499	Although it has been reported that N-glycosylation provides stability of human mucins, most BSM research has been focused on its O-glycans, while N-glycans have not been reported to date.					
31325506	1	54	dep	macromolecular	171:184	arg1	4 MDa					201:205	4 MDa	201:205	4 MDa	201:205	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	3	55	theme	monosaccharide	667:680	arg1	analysis					682:689	monosaccharide analysis	667:689	monosaccharide analysis of BSM	667:696	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	1	56	theme	submaxillary	120:131	arg1	protein					208:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	56	theme	submaxillary	120:131	arg1	BSM					140:142	BSM	140:142	BSM	140:142	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	56	theme	submaxillary	120:131	arg1	mucin					133:137	Bovine submaxillary mucin	113:137	Bovine submaxillary mucin (BSM)	113:143	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	0	57	theme	core-fucosylated	47:62	arg1	glycans					77:83	core-fucosylated and sulfated glycans	47:83	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	3	58	theme	N-glycan	627:634	arg1	component					641:649	a common N-glycan core component	618:649	a common N-glycan core component	618:649	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	3	59	theme	core	636:639	arg1	component					641:649	a common N-glycan core component	618:649	a common N-glycan core component	618:649	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	1	60	gly	heavily-glycosylated	150:169	arg1	protein					208:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	60	gly	heavily-glycosylated	150:169	arg1	mucin					133:137	Bovine submaxillary mucin	113:137	Bovine submaxillary mucin (BSM)	113:143	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	61	theme	abundant	395:402	arg1	O-glycans					404:412	its abundant O-glycans	391:412	its abundant O-glycans	391:412	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	4	62	theme	hybrid-type	1182:1192	arg1	N-glycans					1208:1216	no hybrid-type or sialylated N-glycans	1179:1216	no hybrid-type or sialylated N-glycans	1179:1216	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	5	63	theme	human	1291:1295	arg1	mucins					1297:1302	human mucins	1291:1302	human mucins	1291:1302	Additionally, these are less-branched structures compared to human mucins.					
31325506	4	64	dep	%	936:936	arg1	sum					943:945	the sum	939:945	the sum of the relative quantities of each N-glycan out of the total N-glycans	939:1016	Seventeen N-glycans comprising ten complex-type [Fucose0~2Hexose3~4N-acetylhexosamine1~6Sulfate0~1; 61.1% (the sum of the relative quantities of each N-glycan out of the total N-glycans)], two high-mannose-type (Hexose5~6N-acetylhexosamine2; 12.0%), and five paucimannose type (Fucose0~1Hexose3~4N-acetylhexosamine2~3; 26.9%) were identified, but no hybrid-type or sialylated N-glycans were found.					
31325506	1	65	theme	O-glycans	404:412	arg1	result					381:386	a result	379:386	a result of its abundant O-glycans	379:412	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	3	66	theme	mass	813:816	arg1	spectrometry					818:829	liquid chromatography-tandem mass spectrometry	784:829	liquid chromatography-tandem mass spectrometry	784:829	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	6	67	theme	core	1380:1383	arg1	fucosylated					1385:1395	core fucosylated	1380:1395	core fucosylated	1380:1395	Of these, ten glycans (77.2%), including two sulfated glycans (8.0%), were core fucosylated, which confer unique biological functions to glycoproteins.					
31325506	2	68	theme	mucins	494:499	arg1	stability					475:483	stability	475:483	stability of human mucins	475:499	Although it has been reported that N-glycosylation provides stability of human mucins, most BSM research has been focused on its O-glycans, while N-glycans have not been reported to date.					
31325506	1	69	used	used	223:226	arg2	protein					208:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	a heavily-glycosylated macromolecular (approximately 4 MDa) protein	148:214	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	69	used	used	223:226	arg2	BSM					140:142	BSM	140:142	BSM	140:142	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	1	69	used	used	223:226	arg2	mucin					133:137	Bovine submaxillary mucin	113:137	Bovine submaxillary mucin (BSM)	113:143	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	0	70	gly	core-fucosylated	47:62	arg1	glycans					77:83	core-fucosylated and sulfated glycans	47:83	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	7	71	gly	N-glycosylation	1461:1475	arg2	sites					1477:1481	The N-glycosylation sites	1457:1481	The N-glycosylation sites	1457:1481	The N-glycosylation sites were identified from the analysis of glycopeptides from BSM.					
31325506	1	72	theme	various	231:237	arg1	applications					251:262	various biomaterial applications	231:262	various biomaterial applications in light of its high viscosity and biocompatibility	231:314	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
31325506	0	73	theme	sulfated	68:75	arg1	glycans					77:83	core-fucosylated and sulfated glycans	47:83	core-fucosylated and sulfated glycans but not sialylated glycans	47:110	N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.					
31325506	3	74	theme	relative	745:752	arg1	quantities					754:763	their relative quantities	739:763	their relative quantities	739:763	In this study, a common N-glycan core component was detected by monosaccharide analysis of BSM, and the structures of the N-glycans and their relative quantities were determined by liquid chromatography-tandem mass spectrometry.					
31325506	1	75	theme	biomaterial	239:249	arg1	applications					251:262	various biomaterial applications	231:262	various biomaterial applications in light of its high viscosity and biocompatibility	231:314	Bovine submaxillary mucin (BSM) is a heavily-glycosylated macromolecular (approximately 4 MDa) protein and is used in various biomaterial applications in light of its high viscosity and biocompatibility, in addition to use as a biochemical substrate or inhibitor as a result of its abundant O-glycans.					
30739313	3	0	theme	O-glycans	337:345	arg1	pathway					326:332	The synthetic pathway	312:332	The synthetic pathway of O-glycans	312:345	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	4	1	theme	diverse	518:524	arg1	pathway					503:509	the pathway	499:509	the pathway highly diverse	499:524	The expression pattern of these enzymes is cell and tissue-specific, thus making the pathway highly diverse.					
30739313	1	2	gly	glycoproteins	169:181	arg1	glycoproteins					169:181	glycoproteins	169:181	glycoproteins	169:181	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	1	3	theme	glycoproteins	169:181	arg1	structure					142:150	structure	142:150	structure	142:150	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	1	3	theme	glycoproteins	169:181	arg1	stability					156:164	stability	156:164	stability	156:164	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	7	4	theme	possible	902:909	arg1	glycans					911:917	possible glycans	902:917	possible glycans	902:917	Using this information, the RING generates a list of possible glycans, which is used as input into O-Glycovis.					
30739313	8	5	theme	reaction	1033:1040	arg1	paths					1042:1046	potential reaction paths	1023:1046	potential reaction paths	1023:1046	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	4	6	theme	expression	422:431	arg1	cell					461:464	cell	461:464	cell	461:464	The expression pattern of these enzymes is cell and tissue-specific, thus making the pathway highly diverse.					
30739313	4	6	theme	expression	422:431	arg1	pattern					433:439	The expression pattern	418:439	The expression pattern of these enzymes	418:456	The expression pattern of these enzymes is cell and tissue-specific, thus making the pathway highly diverse.					
30739313	9	7	theme	overlaid	1245:1252	arg1	glycans					1237:1243	glycans	1237:1243	glycans overlaid	1237:1252	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	10	8	theme	O-Glycan	1255:1262	arg1	profiles					1264:1271	O-Glycan profiles	1255:1271	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells	1255:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	9	9	theme	glycan	1087:1092	arg1	data					1094:1097	the input glycan data	1077:1097	the input glycan data	1077:1097	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	3	10	theme	enzymes	374:380	arg1	number					364:369	a large number	356:369	a large number of enzymes	356:380	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	10	11	from	lines	1301:1305	arg1	profiles					1264:1271	O-Glycan profiles	1255:1271	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells	1255:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	12	theme	Chinese	1367:1373	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	12	theme	Chinese	1367:1373	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	6	13	theme	glycan	832:837	arg1	products					839:846	the glycan products	828:846	the glycan products	828:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	1	14	dep	structure	142:150	arg1	the					138:140	the	138:140	the	138:140	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	10	15	theme	vascular	1339:1346	arg1	endothelium					1348:1358	vascular endothelium	1339:1358	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	11	16	theme	different	1639:1647	arg1	tissues					1649:1655	different tissues	1639:1655	different tissues	1639:1655	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	5	17	theme	O-GlycoVis	676:685	arg1	platform					624:631	an integrated platform	610:631	an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis	610:685	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	9	18	theme	pathway	1186:1192	arg1	maps					1194:1197	pathway maps	1186:1197	pathway maps	1186:1197	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	19	from	specificity	758:768	arg1	formation					815:823	the formation	811:823	the formation of the glycan products	811:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	8	20	theme	glycan	984:989	arg1	distribution					991:1002	the glycan distribution	980:1002	the glycan distribution in the pathway and potential reaction paths leading to each glycan	980:1069	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	10	21	theme	cell	1296:1299	arg1	MCF7					1308:1311	MCF7	1308:1311	MCF7	1308:1311	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	21	theme	cell	1296:1299	arg1	T47d					1317:1320	T47d	1317:1320	T47d	1317:1320	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	21	theme	cell	1296:1299	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	21	theme	cell	1296:1299	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	21	theme	cell	1296:1299	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	5	22	theme	RING	636:639	arg1	platform					624:631	an integrated platform	610:631	an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis	610:685	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	2	23	theme	other	299:303	arg1	cells					305:309	other cells	299:309	other cells	299:309	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	9	24	theme	input	1081:1085	arg1	data					1094:1097	the input glycan data	1077:1097	the input glycan data	1077:1097	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	25	theme	products	839:846	arg1	formation					815:823	the formation	811:823	the formation of the glycan products	811:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	5	26	theme	Rule	642:645	arg1	Generator					661:669	Rule Input Network Generator	642:669	Rule Input Network Generator	642:669	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	5	26	theme	Rule	642:645	arg1	RING					636:639	RING	636:639	RING (Rule Input Network Generator)	636:670	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	11	27	theme	tissues	1649:1655	arg1	network					1628:1634	O-glycosylation network	1612:1634	O-glycosylation network of different tissues and species	1612:1667	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	1	28	theme	Mucin-type	92:101	arg1	O-glycans					103:111	Mucin-type O-glycans	92:111	Mucin-type O-glycans	92:111	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	6	29	theme	reaction	714:721	arg1	language					723:730	an English-like reaction language	698:730	an English-like reaction language	698:730	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	11	30	theme	network	1628:1634	arg1	networks					1600:1607	networks	1600:1607	networks of O-glycosylation network of different tissues and species	1600:1667	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	0	31	theme	integrated	3:12	arg1	platform					14:21	An integrated platform	0:21	An integrated platform for mucin-type O-glycosylation	0:52	An integrated platform for mucin-type O-glycosylation network generation and visualization.					
30739313	5	32	theme	Input	647:651	arg1	Generator					661:669	Rule Input Network Generator	642:669	Rule Input Network Generator	642:669	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	5	32	theme	Input	647:651	arg1	RING					636:639	RING	636:639	RING (Rule Input Network Generator)	636:670	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	9	33	theme	reaction	1136:1143	arg1	paths					1145:1149	all possible reaction paths	1123:1149	all possible reaction paths leading to each glycan and outputs pathway maps	1123:1197	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	34	theme	English-like	701:712	arg1	language					723:730	an English-like reaction language	698:730	an English-like reaction language	698:730	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	5	35	theme	Network	653:659	arg1	Generator					661:669	Rule Input Network Generator	642:669	Rule Input Network Generator	642:669	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	5	35	theme	Network	653:659	arg1	RING					636:639	RING	636:639	RING (Rule Input Network Generator)	636:670	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	8	36	theme	potential	1023:1031	arg1	paths					1042:1046	potential reaction paths	1023:1046	potential reaction paths	1023:1046	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	11	37	theme	network	1560:1566	arg1	generation					1568:1577	network generation	1560:1577	network generation	1560:1577	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	3	38	theme	diverse	387:393	arg1	specificity					405:415	diverse substrate specificity	387:415	diverse substrate specificity	387:415	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	9	39	theme	relative	1208:1215	arg1	levels					1227:1232	the relative abundance levels	1204:1232	the relative abundance levels of glycans overlaid	1204:1252	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	40	theme	constraints	796:806	arg1	specificity					758:768	the substrate specificity	744:768	the substrate specificity of enzymes and additional constraints on the formation of the glycan products	744:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	1	41	theme	profound	118:125	arg1	effects					127:133	profound effects	118:133	profound effects	118:133	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	5	42	theme	integrated	613:622	arg1	platform					624:631	an integrated platform	610:631	an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis	610:685	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	3	43	theme	substrate	395:403	arg1	specificity					405:415	diverse substrate specificity	387:415	diverse substrate specificity	387:415	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	2	44	with	interactions	248:259	arg1	cells					305:309	other cells	299:309	other cells	299:309	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	2	44	with	interactions	248:259	arg1	environments					282:293	the surrounding environments	266:293	the surrounding environments	266:293	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	6	45	theme	enzymes	773:779	arg1	specificity					758:768	the substrate specificity	744:768	the substrate specificity of enzymes and additional constraints on the formation of the glycan products	744:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	2	46	theme	cell	201:204	arg1	proteins					214:221	the cell surface proteins	197:221	the cell surface proteins	197:221	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	6	47	from	constraints	796:806	arg1	formation					815:823	the formation	811:823	the formation of the glycan products	811:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	10	48	theme	cancer	1289:1294	arg1	MCF7					1308:1311	MCF7	1308:1311	MCF7	1308:1311	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	48	theme	cancer	1289:1294	arg1	T47d					1317:1320	T47d	1317:1320	T47d	1317:1320	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	48	theme	cancer	1289:1294	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	48	theme	cancer	1289:1294	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	48	theme	cancer	1289:1294	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	49	theme	human	1323:1327	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	49	theme	human	1323:1327	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	6	50	from	formation	815:823	arg1	specificity					758:768	the substrate specificity	744:768	the substrate specificity of enzymes and additional constraints on the formation of the glycan products	744:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	0	51	theme	mucin-type	27:36	arg1	O-glycosylation					38:52	mucin-type O-glycosylation	27:52	mucin-type O-glycosylation	27:52	An integrated platform for mucin-type O-glycosylation network generation and visualization.					
30739313	9	52	theme	glycans	1237:1243	arg1	levels					1227:1232	the relative abundance levels	1204:1232	the relative abundance levels of glycans overlaid	1204:1252	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	53	theme	additional	785:794	arg1	constraints					796:806	additional constraints	785:806	additional constraints on the formation of the glycan products	785:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	5	54	theme	cell	563:566	arg1	fashion					588:594	a cell and tissue-specific fashion	561:594	fashion	588:594	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	10	55	theme	breast	1282:1287	arg1	MCF7					1308:1311	MCF7	1308:1311	MCF7	1308:1311	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	55	theme	breast	1282:1287	arg1	T47d					1317:1320	T47d	1317:1320	T47d	1317:1320	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	55	theme	breast	1282:1287	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	55	theme	breast	1282:1287	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	55	theme	breast	1282:1287	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	56	theme	transcriptional	1419:1433	arg1	data					1435:1438	transcriptional data	1419:1438	transcriptional data	1419:1438	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	2	57	from	O-Glycans	184:192	arg1	proteins					214:221	the cell surface proteins	197:221	the cell surface proteins	197:221	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	9	58	theme	possible	1127:1134	arg1	paths					1145:1149	all possible reaction paths	1123:1149	all possible reaction paths leading to each glycan and outputs pathway maps	1123:1197	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	6	59	from	enzymes	773:779	arg1	formation					815:823	the formation	811:823	the formation of the glycan products	811:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	11	60	theme	species	1661:1667	arg1	network					1628:1634	O-glycosylation network	1612:1634	O-glycosylation network of different tissues and species	1612:1667	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	1	61	contain	have	113:116	arg2	effects					127:133	profound effects	118:133	profound effects	118:133	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	1	61	contain	have	113:116	arg1	O-glycans					103:111	Mucin-type O-glycans	92:111	Mucin-type O-glycans	92:111	Mucin-type O-glycans have profound effects on the structure and stability of glycoproteins.					
30739313	4	62	theme	enzymes	450:456	arg1	cell					461:464	cell	461:464	cell	461:464	The expression pattern of these enzymes is cell and tissue-specific, thus making the pathway highly diverse.					
30739313	4	62	theme	enzymes	450:456	arg1	pattern					433:439	The expression pattern	418:439	The expression pattern of these enzymes	418:456	The expression pattern of these enzymes is cell and tissue-specific, thus making the pathway highly diverse.					
30739313	10	63	theme	observed	1473:1480	arg1	O-glycans					1482:1490	experimentally observed O-glycans	1458:1490	experimentally observed O-glycans	1458:1490	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	5	64	theme	tissue-specific	572:586	arg1	fashion					588:594	a cell and tissue-specific fashion	561:594	fashion	588:594	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	8	65	from	distribution	991:1002	arg1	pathway					1011:1017	pathway	1011:1017	pathway	1011:1017	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	8	65	from	distribution	991:1002	arg1	paths					1042:1046	potential reaction paths	1023:1046	potential reaction paths	1023:1046	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	11	66	theme	RING-based	1498:1507	arg1	program					1509:1515	This RING-based program	1493:1515	This RING-based program	1493:1515	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	9	67	theme	abundance	1217:1225	arg1	levels					1227:1232	the relative abundance levels	1204:1232	the relative abundance levels of glycans overlaid	1204:1252	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	10	68	theme	Ovary	1383:1387	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	68	theme	Ovary	1383:1387	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	9	69	dep	glycan	1167:1172	arg1	maps					1194:1197	pathway maps	1186:1197	pathway maps	1186:1197	With the input glycan data, O-GlycoVis also traces all possible reaction paths leading to each glycan and outputs pathway maps with the relative abundance levels of glycans overlaid.					
30739313	3	70	theme	synthetic	316:324	arg1	pathway					326:332	The synthetic pathway	312:332	The synthetic pathway of O-glycans	312:345	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	2	71	theme	surrounding	270:280	arg1	environments					282:293	the surrounding environments	266:293	the surrounding environments	266:293	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	5	72	theme	pathway	541:547	arg1	analysis					549:556	pathway analysis	541:556	pathway analysis	541:556	To facilitate pathway analysis in a cell and tissue-specific fashion, we developed an integrated platform of RING (Rule Input Network Generator) and O-GlycoVis.					
30739313	7	73	used	used	929:932	arg2	list					894:897	a list	892:897	a list	892:897	Using this information, the RING generates a list of possible glycans, which is used as input into O-Glycovis.					
30739313	7	73	used	used	929:932	arg2	input					937:941	input	937:941	input into O-Glycovis	937:957	Using this information, the RING generates a list of possible glycans, which is used as input into O-Glycovis.					
30739313	7	74	theme	glycans	911:917	arg1	input					937:941	input	937:941	input into O-Glycovis	937:957	Using this information, the RING generates a list of possible glycans, which is used as input into O-Glycovis.					
30739313	7	74	theme	glycans	911:917	arg1	list					894:897	a list	892:897	a list	892:897	Using this information, the RING generates a list of possible glycans, which is used as input into O-Glycovis.					
30739313	8	75	dep	pathway	1011:1017	arg1	the					1007:1009	the	1007:1009	the	1007:1009	O-GlycoVis displays the glycan distribution in the pathway and potential reaction paths leading to each glycan.					
30739313	2	76	theme	surface	206:212	arg1	proteins					214:221	the cell surface proteins	197:221	the cell surface proteins	197:221	O-Glycans on the cell surface proteins also modulate the cell's interactions with the surrounding environments and other cells.					
30739313	6	77	theme	substrate	748:756	arg1	specificity					758:768	the substrate specificity	744:768	the substrate specificity of enzymes and additional constraints on the formation of the glycan products	744:846	RING uses an English-like reaction language to describe the substrate specificity of enzymes and additional constraints on the formation of the glycan products.					
30739313	10	78	theme	Hamster	1375:1381	arg1	cells					1389:1393	Chinese Hamster Ovary cells	1367:1393	Chinese Hamster Ovary cells	1367:1393	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	78	theme	Hamster	1375:1381	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	11	79	theme	networks	1600:1607	arg1	generation					1568:1577	network generation	1560:1577	network generation	1560:1577	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	11	79	theme	networks	1600:1607	arg1	visualization					1583:1595	visualization	1583:1595	visualization	1583:1595	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	3	80	theme	large	358:362	arg1	number					364:369	a large number	356:369	a large number of enzymes	356:380	The synthetic pathway of O-glycans involves a large number of enzymes with diverse substrate specificity.					
30739313	11	81	theme	O-glycosylation	1612:1626	arg1	network					1628:1634	O-glycosylation network	1612:1634	O-glycosylation network of different tissues and species	1612:1667	This RING-based program allows rules to be added or subtracted for network generation and visualization of networks of O-glycosylation network of different tissues and species.					
30739313	10	82	theme	endothelium	1348:1358	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	82	theme	endothelium	1348:1358	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	83	theme	umbilical	1329:1337	arg1	cells					1360:1364	human umbilical vascular endothelium cells	1323:1364	human umbilical vascular endothelium cells	1323:1364	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
30739313	10	83	theme	umbilical	1329:1337	arg1	lines					1301:1305	two breast cancer cell lines	1278:1305	two breast cancer cell lines	1278:1305	O-Glycan profiles from two breast cancer cell lines, MCF7 and T47d, human umbilical vascular endothelium cells, Chinese Hamster Ovary cells were generated based on transcriptional data and compared with experimentally observed O-glycans.					
31557542	0	0	theme	human	74:78	arg1	sialyltransferases					87:104	human ST3GAL sialyltransferases	74:104	human ST3GAL sialyltransferases	74:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	7	1	theme	N-glycosylation	1008:1022	arg1	positions					1024:1032	the first time N-glycosylation positions	993:1032	the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	993:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	2	2	theme	acid	358:361	arg1	addition					363:370	the terminal sialic acid addition	338:370	the terminal sialic acid addition from CMP donor to the glycan units	338:405	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	3	3	dep	ST3Gal	578:583	arg1	VI					585:586	VI	585:586	VI	585:586	ST3GAL family is one of the most important STs and divided into the six subfamily in mouse and humans which are ST3Gal I, ST3Gal II, ST3Gal III, ST3Gal IV, ST3Gal V, and ST3Gal VI.					
31557542	6	4	theme	family	944:949	arg1	glycosylation					893:905	glycosylation	893:905	glycosylation	893:905	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	6	4	theme	family	944:949	arg1	structure					882:890	structure	882:890	structure	882:890	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	6	4	theme	family	944:949	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	6	4	theme	family	944:949	arg1	site					923:926	CMP binding site	911:926	CMP binding site	911:926	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	7	5	from	IV	1044:1045	arg1	ST3Gal					1083:1088	ST3Gal III	1083:1092	ST3Gal III	1083:1092	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	5	from	IV	1044:1045	arg1	O-GlcNAcylation					1098:1112	O-GlcNAcylation	1098:1112	O-GlcNAcylation	1098:1112	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	5	from	IV	1044:1045	arg1	V					1124:1124	ST3Gal V	1117:1124	ST3Gal V	1117:1124	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	5	from	IV	1044:1045	arg1	relation					1136:1143	their relation	1130:1143	their relation with sialylmotifs	1130:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	2	6	theme	sialic	351:356	arg1	addition					363:370	the terminal sialic acid addition	338:370	the terminal sialic acid addition from CMP donor to the glycan units	338:405	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	7	7	with	V	1124:1124	arg1	sialylmotifs					1150:1161	sialylmotifs	1150:1161	sialylmotifs	1150:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	0	8	theme	sialyltransferases	87:104	arg1	glycosylation					41:53	glycosylation	41:53	glycosylation	41:53	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	0	8	theme	sialyltransferases	87:104	arg1	binding					63:69	CMP binding	59:69	CMP binding	59:69	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	0	8	theme	sialyltransferases	87:104	arg1	structure					30:38	structure	30:38	structure	30:38	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	5	9	dep	are	724:726	arg1	is					786:787	is	786:787	is a paucity of information about structure of human ST3GAL family	786:851	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	8	10	theme	ST3GAL	1221:1226	arg1	family					1235:1240	human ST3GAL enzyme family	1215:1240	human ST3GAL enzyme family	1215:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	1	11	theme	cell-cell	254:262	arg1	interactions					282:293	cell-cell and host-pathogen interactions	254:293	cell-cell and host-pathogen interactions	254:293	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	0	12	theme	ST3GAL	80:85	arg1	sialyltransferases					87:104	human ST3GAL sialyltransferases	74:104	human ST3GAL sialyltransferases	74:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	5	13	from	reports	733:739	arg1	function					755:762	the ST3GAL function	744:762	the ST3GAL function in mammals	744:773	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	7	14	theme	time	1003:1006	arg1	positions					1024:1032	the first time N-glycosylation positions	993:1032	the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	993:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	15	with	relation	1136:1143	arg1	sialylmotifs					1150:1161	sialylmotifs	1150:1161	sialylmotifs	1150:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	4	16	theme	ST3GAL	608:613	arg1	family					615:620	the ST3GAL family	604:620	the ST3GAL family	604:620	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	5	17	theme	information	802:812	arg1	paucity					791:797	a paucity	789:797	a paucity of information about structure of human ST3GAL family	789:851	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	5	18	theme	ST3GAL	748:753	arg1	function					755:762	the ST3GAL function	744:762	the ST3GAL function in mammals	744:773	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	8	19	theme	binding	1359:1365	arg1	positions					1367:1375	the CMP binding positions	1351:1375	the CMP binding positions in ST3Gal III-VI subfamilies	1351:1404	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	6	20	theme	ST3GAL	937:942	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	7	21	theme	ST3Gal	1037:1042	arg1	IV					1044:1045	ST3Gal IV	1037:1045	ST3Gal IV	1037:1045	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	8	22	theme	enzyme	1228:1233	arg1	family					1235:1240	human ST3GAL enzyme family	1215:1240	human ST3GAL enzyme family	1215:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	4	23	theme	glycochains	681:691	arg1	glycochains					681:691	glycochains	681:691	glycochains through an α2,3-linkage	681:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	4	23	theme	glycochains	681:691	arg1	residues					669:676	the terminal galactose residues	646:676	the terminal galactose residues of glycochains through an α2,3-linkage	646:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	7	24	theme	mucin	1055:1059	arg1	glycosylation					1066:1078	mucin type glycosylation	1055:1078	VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	1051:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	5	25	theme	human	833:837	arg1	family					846:851	human ST3GAL family	833:851	human ST3GAL family	833:851	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	6	26	theme	computational	957:969	arg1	methods					971:977	computational methods	957:977	computational methods	957:977	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	0	27	theme	Computational	0:12	arg1	analysis					14:21	Computational analysis	0:21	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases	0:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	1	28	theme	host-pathogen	268:280	arg1	interactions					282:293	cell-cell and host-pathogen interactions	254:293	cell-cell and host-pathogen interactions	254:293	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	8	29	from	positions	1367:1375	arg1	subfamilies					1394:1404	III-VI subfamilies	1387:1404	ST3Gal III-VI subfamilies	1380:1404	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	5	30	theme	ST3GAL	839:844	arg1	family					846:851	human ST3GAL family	833:851	human ST3GAL family	833:851	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	2	31	theme	CMP	377:379	arg1	donor					381:385	CMP donor	377:385	CMP donor	377:385	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	5	32	theme	many	728:731	arg1	reports					733:739	many reports	728:739	many reports on the ST3GAL function in mammals	728:773	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	8	33	theme	CMP	1355:1357	arg1	positions					1367:1375	the CMP binding positions	1351:1375	the CMP binding positions in ST3Gal III-VI subfamilies	1351:1404	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	4	34	theme	galactose	659:667	arg1	glycochains					681:691	glycochains	681:691	glycochains through an α2,3-linkage	681:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	4	34	theme	galactose	659:667	arg1	residues					669:676	the terminal galactose residues	646:676	the terminal galactose residues of glycochains through an α2,3-linkage	646:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	1	35	theme	biological	186:195	arg1	processes					197:205	many biological processes	181:205	many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer	181:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	35	theme	biological	186:195	arg1	metastasis					299:308	metastasis	299:308	metastasis of cancer	299:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	35	theme	biological	186:195	arg1	signalling					220:229	cell signalling	215:229	cell signalling	215:229	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	35	theme	biological	186:195	arg1	recognition					241:251	cellular recognition	232:251	cellular recognition	232:251	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	35	theme	biological	186:195	arg1	interactions					282:293	cell-cell and host-pathogen interactions	254:293	cell-cell and host-pathogen interactions	254:293	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	8	36	theme	three-dimensional	1245:1261	arg1	structure					1263:1271	three-dimensional structure	1245:1271	three-dimensional structure using molecular docking	1245:1295	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	8	37	theme	family	1235:1240	arg1	positions					1202:1210	CMP binding positions	1190:1210	CMP binding positions of human ST3GAL enzyme family	1190:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	7	38	from	glycosylation	1066:1078	arg1	ST3Gal					1083:1088	ST3Gal III	1083:1092	ST3Gal III	1083:1092	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	38	from	glycosylation	1066:1078	arg1	O-GlcNAcylation					1098:1112	O-GlcNAcylation	1098:1112	O-GlcNAcylation	1098:1112	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	38	from	glycosylation	1066:1078	arg1	V					1124:1124	ST3Gal V	1117:1124	ST3Gal V	1117:1124	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	38	from	glycosylation	1066:1078	arg1	relation					1136:1143	their relation	1130:1143	their relation with sialylmotifs	1130:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	4	39	theme	terminal	650:657	arg1	glycochains					681:691	glycochains	681:691	glycochains through an α2,3-linkage	681:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	4	39	theme	terminal	650:657	arg1	residues					669:676	the terminal galactose residues	646:676	the terminal galactose residues of glycochains through an α2,3-linkage	646:715	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	3	40	theme	STs	451:453	arg1	one					425:427	one	425:427	one	425:427	ST3GAL family is one of the most important STs and divided into the six subfamily in mouse and humans which are ST3Gal I, ST3Gal II, ST3Gal III, ST3Gal IV, ST3Gal V, and ST3Gal VI.					
31557542	3	40	theme	STs	451:453	arg1	STs					451:453	the most important STs	432:453	the most important STs	432:453	ST3GAL family is one of the most important STs and divided into the six subfamily in mouse and humans which are ST3Gal I, ST3Gal II, ST3Gal III, ST3Gal IV, ST3Gal V, and ST3Gal VI.					
31557542	7	41	theme	type	1061:1064	arg1	glycosylation					1066:1078	mucin type glycosylation	1055:1078	VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	1051:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	1	42	theme	many	181:184	arg1	processes					197:205	many biological processes	181:205	many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer	181:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	42	theme	many	181:184	arg1	metastasis					299:308	metastasis	299:308	metastasis of cancer	299:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	42	theme	many	181:184	arg1	signalling					220:229	cell signalling	215:229	cell signalling	215:229	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	42	theme	many	181:184	arg1	recognition					241:251	cellular recognition	232:251	cellular recognition	232:251	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	42	theme	many	181:184	arg1	interactions					282:293	cell-cell and host-pathogen interactions	254:293	cell-cell and host-pathogen interactions	254:293	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	6	43	theme	human	931:935	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	0	44	theme	structure	30:38	arg1	analysis					14:21	Computational analysis	0:21	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases	0:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	3	45	theme	ST3GAL	408:413	arg1	family					415:420	ST3GAL family	408:420	ST3GAL family	408:420	ST3GAL family is one of the most important STs and divided into the six subfamily in mouse and humans which are ST3Gal I, ST3Gal II, ST3Gal III, ST3Gal IV, ST3Gal V, and ST3Gal VI.					
31557542	8	46	theme	CMP	1190:1192	arg1	positions					1202:1210	CMP binding positions	1190:1210	CMP binding positions of human ST3GAL enzyme family	1190:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	8	47	theme	molecular	1279:1287	arg1	docking					1289:1295	molecular docking	1279:1295	molecular docking	1279:1295	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	2	48	from	donor	381:385	arg1	addition					363:370	the terminal sialic acid addition	338:370	the terminal sialic acid addition from CMP donor to the glycan units	338:405	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	2	49	theme	glycan	394:399	arg1	units					401:405	the glycan units	390:405	the glycan units	390:405	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	1	50	theme	cancer	313:318	arg1	metastasis					299:308	metastasis	299:308	metastasis of cancer	299:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	50	theme	cancer	313:318	arg1	signalling					220:229	cell signalling	215:229	cell signalling	215:229	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	50	theme	cancer	313:318	arg1	recognition					241:251	cellular recognition	232:251	cellular recognition	232:251	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	50	theme	cancer	313:318	arg1	interactions					282:293	cell-cell and host-pathogen interactions	254:293	cell-cell and host-pathogen interactions	254:293	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	7	51	dep	glycosylation	1066:1078	arg1	VI					1051:1052	VI	1051:1052	VI	1051:1052	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	52	theme	ST3Gal	1117:1122	arg1	V					1124:1124	ST3Gal V	1117:1124	ST3Gal V	1117:1124	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	6	53	gly	glycosylation	893:905	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	2	54	theme	terminal	342:349	arg1	addition					363:370	the terminal sialic acid addition	338:370	the terminal sialic acid addition from CMP donor to the glycan units	338:405	All STs catalyse the terminal sialic acid addition from CMP donor to the glycan units.					
31557542	0	55	theme	glycosylation	41:53	arg1	analysis					14:21	Computational analysis	0:21	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases	0:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	0	56	dep	structure	30:38	arg1	the					26:28	the	26:28	the	26:28	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	4	57	theme	family	615:620	arg1	members					593:599	The members	589:599	The members of the ST3GAL family	589:620	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	7	58	from	positions	1024:1032	arg1	IV					1044:1045	ST3Gal IV	1037:1045	ST3Gal IV	1037:1045	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	58	from	positions	1024:1032	arg1	glycosylation					1066:1078	mucin type glycosylation	1055:1078	VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	1051:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	5	59	theme	family	846:851	arg1	structure					820:828	structure	820:828	structure of human ST3GAL family	820:851	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	1	60	theme	cell	215:218	arg1	signalling					220:229	cell signalling	215:229	cell signalling	215:229	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	7	61	gly	glycosylation	1066:1078	arg1	ST3Gal					1083:1088	ST3Gal III	1083:1092	ST3Gal III	1083:1092	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	61	gly	glycosylation	1066:1078	arg1	O-GlcNAcylation					1098:1112	O-GlcNAcylation	1098:1112	O-GlcNAcylation	1098:1112	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	61	gly	glycosylation	1066:1078	arg1	V					1124:1124	ST3Gal V	1117:1124	ST3Gal V	1117:1124	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	7	61	gly	glycosylation	1066:1078	arg1	relation					1136:1143	their relation	1130:1143	their relation with sialylmotifs	1130:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	5	62	from	function	755:762	arg1	mammals					767:773	mammals	767:773	mammals	767:773	There are many reports on the ST3GAL function in mammals but, there is a paucity of information about structure of human ST3GAL family.					
31557542	7	63	theme	first	997:1001	arg1	positions					1024:1032	the first time N-glycosylation positions	993:1032	the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs	993:1161	We found for the first time N-glycosylation positions in ST3Gal IV and VI, mucin type glycosylation in ST3Gal III and O-GlcNAcylation in ST3Gal V and their relation with sialylmotifs.					
31557542	4	64	theme	sialic	631:636	arg1	acid					638:641	sialic acid	631:641	sialic acid	631:641	The members of the ST3GAL family transfer sialic acid to the terminal galactose residues of glycochains through an α2,3-linkage.					
31557542	8	65	theme	human	1215:1219	arg1	family					1235:1240	human ST3GAL enzyme family	1215:1240	human ST3GAL enzyme family	1215:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	6	66	theme	binding	915:921	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	6	66	theme	binding	915:921	arg1	site					923:926	CMP binding site	911:926	CMP binding site	911:926	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	0	67	theme	binding	63:69	arg1	analysis					14:21	Computational analysis	0:21	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases	0:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	8	68	theme	ST3Gal	1380:1385	arg1	subfamilies					1394:1404	III-VI subfamilies	1387:1404	ST3Gal III-VI subfamilies	1380:1404	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	0	69	gly	glycosylation	41:53	arg1	sialyltransferases					87:104	human ST3GAL sialyltransferases	74:104	human ST3GAL sialyltransferases	74:104	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	3	70	theme	important	441:449	arg1	STs					451:453	the most important STs	432:453	the most important STs	432:453	ST3GAL family is one of the most important STs and divided into the six subfamily in mouse and humans which are ST3Gal I, ST3Gal II, ST3Gal III, ST3Gal IV, ST3Gal V, and ST3Gal VI.					
31557542	8	71	theme	binding	1194:1200	arg1	positions					1202:1210	CMP binding positions	1190:1210	CMP binding positions of human ST3GAL enzyme family	1190:1240	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	6	72	theme	CMP	911:913	arg1	family					944:949	human ST3GAL family	931:949	human ST3GAL family	931:949	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	6	72	theme	CMP	911:913	arg1	site					923:926	CMP binding site	911:926	CMP binding site	911:926	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	0	73	theme	CMP	59:61	arg1	binding					63:69	CMP binding	59:69	CMP binding	59:69	Computational analysis of the structure, glycosylation and CMP binding of human ST3GAL sialyltransferases.					
31557542	6	74	dep	structure	882:890	arg1	the					878:880	the	878:880	the	878:880	Herein, we investigated the structure, glycosylation and CMP binding site of human ST3GAL family using computational methods.					
31557542	8	75	theme	sialylmotifs	1324:1335	arg1	relation					1337:1344	the sialylmotifs relation	1320:1344	the sialylmotifs relation	1320:1344	In addition, we predicted CMP binding positions of human ST3GAL enzyme family on three-dimensional structure using molecular docking and first demonstrated the sialylmotifs relation with the CMP binding positions in ST3Gal III-VI subfamilies.					
31557542	1	76	theme	fundamental	140:150	arg1	enzymes					152:158	the fundamental enzymes	136:158	the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer	136:318	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	76	theme	fundamental	140:150	arg1	related					170:176	related	170:176	related	170:176	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	76	theme	fundamental	140:150	arg1	Sialyltransferases					107:124	Sialyltransferases	107:124	Sialyltransferases (STs)	107:130	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31557542	1	77	theme	cellular	232:239	arg1	recognition					241:251	cellular recognition	232:251	cellular recognition	232:251	Sialyltransferases (STs) are the fundamental enzymes which are related to many biological processes such as cell signalling, cellular recognition, cell-cell and host-pathogen interactions and metastasis of cancer.					
31719148	4	0	theme	heterogeneous	877:889	arg1	patterns					905:912	heterogeneous glycosylation patterns	877:912	heterogeneous glycosylation patterns	877:912	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	9	1	theme	structural	1984:1993	arg1	characteristics					1995:2009	other structural characteristics	1978:2009	other structural characteristics	1978:2009	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	2	2	theme	C-type	381:386	arg1	receptors					402:410	the C-type lectin immune receptors	377:410	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	3	theme	innate	316:321	arg1	receptors					330:338	glycan-recognizing innate immune receptors	297:338	glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs)	297:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	4	4	theme	dodecameric	839:849	arg1	structures					862:871	complex dodecameric quaternary structures	831:871	complex dodecameric quaternary structures	831:871	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	1	5	theme	nonspecific	181:191	arg1	immunostimulants					193:208	nonspecific immunostimulants	181:208	nonspecific immunostimulants in cancer	181:218	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	1	5	theme	nonspecific	181:191	arg1	Hemocyanins					125:135	Hemocyanins	125:135	Hemocyanins	125:135	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	8	6	gly	N-deglycosylated	1774:1789	arg1	hemocyanins					1791:1801	N-deglycosylated hemocyanins	1774:1801	N-deglycosylated hemocyanins	1774:1801	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	2	7	theme	antigen-presenting	343:360	arg1	receptors					488:496	the Toll-like receptors	474:496	the Toll-like receptors (TLRs)	474:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	7	theme	antigen-presenting	343:360	arg1	receptors					402:410	the C-type lectin immune receptors	377:410	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	7	theme	antigen-presenting	343:360	arg1	lectin					456:461	macrophage galactose lectin	435:461	macrophage galactose lectin (MGL)	435:467	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	7	theme	antigen-presenting	343:360	arg1	cells					362:366	antigen-presenting cells	343:366	antigen-presenting cells	343:366	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	6	8	theme	secondary	1197:1205	arg1	structure					1207:1215	hemocyanin secondary structure	1186:1215	hemocyanin secondary structure	1186:1215	Biochemical analyses revealed that the deglycosylation does not change hemocyanin secondary structure but alters their refolding mechanism and dodecameric structure.					
31719148	5	9	theme	immunogenic	1094:1104	arg1	effects					1106:1112	their immunogenic effects	1088:1112	their immunogenic effects	1088:1112	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	7	10	theme	necrosis	1475:1482	arg1	factor					1484:1489	tumor necrosis factor α	1469:1491	tumor necrosis factor α	1469:1491	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	4	11	gly	glycoproteins	812:824	arg1	concholepas					715:725	Concholepas concholepas	703:725	Concholepas concholepas (CCH)	703:731	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	11	gly	glycoproteins	812:824	arg1	hemocyanins					682:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	11	gly	glycoproteins	812:824	arg1	latimarginata					745:757	Fissurella latimarginata	734:757	Fissurella latimarginata (FLH)	734:763	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	11	gly	glycoproteins	812:824	arg1	glycoproteins					812:824	oligomeric glycoproteins	801:824	oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans	801:960	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	11	gly	glycoproteins	812:824	arg1	crenulata					780:788	Megathura crenulata	770:788	Megathura crenulata (KLH)	770:794	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	0	12	from	stability	71:79	arg1	mammals					116:122	mammals	116:122	mammals	116:122	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	7	13	theme	impaired	1446:1453	arg1	production					1455:1464	an impaired production	1443:1464	an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages	1443:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	9	14	theme	biomedical	2095:2104	arg1	applications					2106:2117	biomedical applications	2095:2117	biomedical applications	2095:2117	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	4	15	theme	Fissurella	734:743	arg1	FLH					760:762	FLH	760:762	FLH	760:762	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	15	theme	Fissurella	734:743	arg1	latimarginata					745:757	Fissurella latimarginata	734:757	Fissurella latimarginata (FLH)	734:763	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	3	16	from	role	567:570	arg1	properties					637:646	the structural and immunological properties	604:646	the structural and immunological properties of hemocyanin	604:660	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	7	17	with	concomitant	1426:1436	arg1	production					1455:1464	an impaired production	1443:1464	an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages	1443:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	18	theme	altered	1891:1897	arg1	activities					1907:1916	altered carrier activities	1891:1916	altered carrier activities	1891:1916	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	5	19	gly	N-deglycosylation	1001:1017	arg1	FLH					1027:1029	FLH	1027:1029	FLH	1027:1029	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	5	19	gly	N-deglycosylation	1001:1017	arg1	KLH					1036:1038	KLH	1036:1038	KLH	1036:1038	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	5	19	gly	N-deglycosylation	1001:1017	arg1	CCH					1022:1024	CCH	1022:1024	CCH	1022:1024	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	6	20	theme	dodecameric	1258:1268	arg1	structure					1270:1278	dodecameric structure	1258:1278	dodecameric structure	1258:1278	Biochemical analyses revealed that the deglycosylation does not change hemocyanin secondary structure but alters their refolding mechanism and dodecameric structure.					
31719148	4	21	theme	Concholepas	703:713	arg1	concholepas					715:725	Concholepas concholepas	703:725	Concholepas concholepas (CCH)	703:731	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	21	theme	Concholepas	703:713	arg1	CCH					728:730	CCH	728:730	CCH	728:730	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	7	22	theme	decreased	1315:1323	arg1	binding					1325:1331	decreased binding	1315:1331	decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors	1315:1391	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	3	23	theme	immunological	623:635	arg1	properties					637:646	the structural and immunological properties	604:646	the structural and immunological properties of hemocyanin	604:660	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	8	24	gly	N-deglycosylated	1597:1612	arg1	hemocyanins					1614:1624	N-deglycosylated hemocyanins	1597:1624	N-deglycosylated hemocyanins	1597:1624	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	0	25	theme	immunomodulatory	85:100	arg1	properties					102:111	immunomodulatory properties	85:111	immunomodulatory properties	85:111	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	1	26	from	adjuvants	166:174	arg1	cancer					213:218	cancer	213:218	cancer	213:218	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	7	27	dep	interleukins	1498:1509	arg1	IL-12p40					1530:1537	IL-12p40	1530:1537	IL-12p40	1530:1537	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	7	27	dep	interleukins	1498:1509	arg1	IL-6					1521:1524	IL-6	1521:1524	IL-6	1521:1524	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	7	27	dep	interleukins	1498:1509	arg1	interleukins					1498:1509	interleukins 6 and 12	1498:1518	interleukins	1498:1509	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	2	28	theme	cytokine	534:541	arg1	secretion					543:551	proinflammatory cytokine secretion	518:551	proinflammatory cytokine secretion	518:551	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	1	29	from	immunostimulants	193:208	arg1	cancer					213:218	cancer	213:218	cancer	213:218	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	1	30	from	carriers	156:163	arg1	cancer					213:218	cancer	213:218	cancer	213:218	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	2	31	dep	receptors	402:410	arg1	MR					430:431	MR	430:431	MR	430:431	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	31	dep	receptors	402:410	arg1	receptor					420:427	mannose receptor	412:427	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	5	32	theme	CCH	1022:1024	arg1	N-deglycosylation					1001:1017	enzyme-catalyzed N-deglycosylation	984:1017	enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH	984:1038	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	7	33	theme	concomitant	1426:1436	arg1	endocytosis					1414:1424	endocytosis	1414:1424	endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages	1414:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	34	theme	hemocyanins	1614:1624	arg1	function					1585:1592	the function	1581:1592	the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects	1581:1695	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	8	35	dep	elicited	1803:1810	arg1	reduced					1812:1818	reduced	1812:1818	reduced antibody titers	1812:1834	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	0	36	gly	N-Glycosylation	0:14	arg1	hemocyanins					27:37	mollusk hemocyanins	19:37	mollusk hemocyanins	19:37	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	2	37	theme	galactose	446:454	arg1	MGL					464:466	MGL	464:466	MGL	464:466	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	37	theme	galactose	446:454	arg1	lectin					456:461	macrophage galactose lectin	435:461	macrophage galactose lectin (MGL)	435:467	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	0	38	theme	mollusk	19:25	arg1	hemocyanins					27:37	mollusk hemocyanins	19:37	mollusk hemocyanins	19:37	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	7	39	gly	N-deglycosylated	1336:1351	arg1	hemocyanins					1353:1363	N-deglycosylated hemocyanins	1336:1363	N-deglycosylated hemocyanins	1336:1363	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	2	40	theme	Toll-like	478:486	arg1	TLRs					499:502	TLRs	499:502	TLRs	499:502	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	40	theme	Toll-like	478:486	arg1	receptors					488:496	the Toll-like receptors	474:496	the Toll-like receptors (TLRs)	474:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	8	41	theme	immune	1641:1646	arg1	response					1648:1655	the humoral immune response	1629:1655	the humoral immune response	1629:1655	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	4	42	theme	oligomeric	801:810	arg1	concholepas					715:725	Concholepas concholepas	703:725	Concholepas concholepas (CCH)	703:731	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	42	theme	oligomeric	801:810	arg1	hemocyanins					682:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	42	theme	oligomeric	801:810	arg1	latimarginata					745:757	Fissurella latimarginata	734:757	Fissurella latimarginata (FLH)	734:763	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	42	theme	oligomeric	801:810	arg1	glycoproteins					812:824	oligomeric glycoproteins	801:824	oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans	801:960	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	42	theme	oligomeric	801:810	arg1	crenulata					780:788	Megathura crenulata	770:788	Megathura crenulata (KLH)	770:794	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	5	43	theme	quaternary	1055:1064	arg1	structure					1066:1074	their quaternary structure	1049:1074	their quaternary structure	1049:1074	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	9	44	theme	hemocyanins	1956:1966	arg1	content					1945:1951	the glycan content	1934:1951	the glycan content of hemocyanins	1934:1966	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	8	45	theme	nonspecific	1667:1677	arg1	effects					1689:1695	their nonspecific antitumor effects	1661:1695	their nonspecific antitumor effects	1661:1695	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	1	46	theme	Th1	241:243	arg1	immunity					245:252	Th1 immunity	241:252	Th1 immunity	241:252	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	5	47	theme	FLH	1027:1029	arg1	N-deglycosylation					1001:1017	enzyme-catalyzed N-deglycosylation	984:1017	enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH	984:1038	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	7	48	theme	hemocyanins	1353:1363	arg1	binding					1325:1331	decreased binding	1315:1331	decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors	1315:1391	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	7	48	theme	hemocyanins	1353:1363	arg1	TLR4					1397:1400	TLR4	1397:1400	TLR4	1397:1400	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	9	49	theme	other	1978:1982	arg1	characteristics					1995:2009	other structural characteristics	1978:2009	other structural characteristics	1978:2009	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	2	50	theme	lectin	388:393	arg1	receptors					402:410	the C-type lectin immune receptors	377:410	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	8	51	theme	native	1755:1760	arg1	hemocyanins					1762:1772	native hemocyanins	1755:1772	native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects	1755:1885	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	8	52	theme	melanoma	1711:1718	arg1	model					1720:1724	the B16F10 melanoma model	1700:1724	the B16F10 melanoma model	1700:1724	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	2	53	theme	immune	323:328	arg1	receptors					330:338	glycan-recognizing innate immune receptors	297:338	glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs)	297:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	8	54	theme	N-deglycosylated	1774:1789	arg1	hemocyanins					1791:1801	N-deglycosylated hemocyanins	1774:1801	N-deglycosylated hemocyanins	1774:1801	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	4	55	theme	glycosylation	891:903	arg1	patterns					905:912	heterogeneous glycosylation patterns	877:912	heterogeneous glycosylation patterns	877:912	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	6	56	theme	refolding	1234:1242	arg1	mechanism					1244:1252	their refolding mechanism	1228:1252	their refolding mechanism	1228:1252	Biochemical analyses revealed that the deglycosylation does not change hemocyanin secondary structure but alters their refolding mechanism and dodecameric structure.					
31719148	2	57	theme	glycan-recognizing	297:314	arg1	receptors					330:338	glycan-recognizing innate immune receptors	297:338	glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs)	297:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	4	58	theme	complex	831:837	arg1	structures					862:871	complex dodecameric quaternary structures	831:871	complex dodecameric quaternary structures	831:871	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	59	theme	Mollusk	674:680	arg1	concholepas					715:725	Concholepas concholepas	703:725	Concholepas concholepas (CCH)	703:731	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	59	theme	Mollusk	674:680	arg1	hemocyanins					682:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins	674:692	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	59	theme	Mollusk	674:680	arg1	latimarginata					745:757	Fissurella latimarginata	734:757	Fissurella latimarginata (FLH)	734:763	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	59	theme	Mollusk	674:680	arg1	glycoproteins					812:824	oligomeric glycoproteins	801:824	oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans	801:960	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	59	theme	Mollusk	674:680	arg1	crenulata					780:788	Megathura crenulata	770:788	Megathura crenulata (KLH)	770:794	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	8	60	theme	antibody	1820:1827	arg1	titers					1829:1834	antibody titers	1820:1834	antibody titers	1820:1834	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	4	61	theme	quaternary	851:860	arg1	structures					862:871	complex dodecameric quaternary structures	831:871	complex dodecameric quaternary structures	831:871	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	1	62	used	used	148:151	arg2	Hemocyanins					125:135	Hemocyanins	125:135	Hemocyanins	125:135	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	1	62	used	used	148:151	arg2	adjuvants					166:174	adjuvants	166:174	adjuvants	166:174	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	1	62	used	used	148:151	arg2	carriers					156:163	carriers	156:163	carriers	156:163	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	1	62	used	used	148:151	arg2	immunostimulants					193:208	nonspecific immunostimulants	181:208	nonspecific immunostimulants in cancer	181:218	Hemocyanins are widely used as carriers, adjuvants, and nonspecific immunostimulants in cancer because they promote Th1 immunity in mammals.					
31719148	9	63	theme	immunological	2042:2054	arg1	activities					2056:2065	their immunological activities	2036:2065	their immunological activities	2036:2065	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	7	64	theme	interleukins	1498:1509	arg1	production					1455:1464	an impaired production	1443:1464	an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages	1443:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	4	65	theme	mannose-rich	939:950	arg1	N-glycans					952:960	mannose-rich N-glycans	939:960	mannose-rich N-glycans	939:960	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	0	66	from	properties	102:111	arg1	mammals					116:122	mammals	116:122	mammals	116:122	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	6	67	theme	hemocyanin	1186:1195	arg1	structure					1207:1215	hemocyanin secondary structure	1186:1215	hemocyanin secondary structure	1186:1215	Biochemical analyses revealed that the deglycosylation does not change hemocyanin secondary structure but alters their refolding mechanism and dodecameric structure.					
31719148	7	68	theme	factor	1484:1489	arg1	production					1455:1464	an impaired production	1443:1464	an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages	1443:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	3	69	theme	oligosaccharides	584:599	arg1	unclear					665:671	unclear	665:671	unclear	665:671	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	3	69	theme	oligosaccharides	584:599	arg1	role					567:570	the role	563:570	the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin	563:660	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	7	70	theme	tumor	1469:1473	arg1	factor					1484:1489	tumor necrosis factor α	1469:1491	tumor necrosis factor α	1469:1491	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	71	dep	hemocyanins	1762:1772	arg1	elicited					1803:1810	elicited	1803:1810	elicited	1803:1810	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	8	71	dep	hemocyanins	1762:1772	arg1	diminished					1858:1867	diminished	1858:1867	partially diminished antitumor effects	1848:1885	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	8	72	theme	antitumor	1869:1877	arg1	effects					1879:1885	antitumor effects	1869:1885	antitumor effects	1869:1885	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	4	73	with	glycoproteins	812:824	arg1	structures					862:871	complex dodecameric quaternary structures	831:871	complex dodecameric quaternary structures	831:871	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	73	with	glycoproteins	812:824	arg1	patterns					905:912	heterogeneous glycosylation patterns	877:912	heterogeneous glycosylation patterns	877:912	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	3	74	theme	N-linked	575:582	arg1	oligosaccharides					584:599	N-linked oligosaccharides	575:599	N-linked oligosaccharides	575:599	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	7	75	theme	Immunochemical	1281:1294	arg1	analyses					1296:1303	Immunochemical analyses	1281:1303	Immunochemical analyses	1281:1303	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	76	theme	carrier	1899:1905	arg1	activities					1907:1916	altered carrier activities	1891:1916	altered carrier activities	1891:1916	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	2	77	from	receptors	330:338	arg1	receptors					488:496	the Toll-like receptors	474:496	the Toll-like receptors (TLRs)	474:503	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	77	from	receptors	330:338	arg1	receptors					402:410	the C-type lectin immune receptors	377:410	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	77	from	receptors	330:338	arg1	lectin					456:461	macrophage galactose lectin	435:461	macrophage galactose lectin (MGL)	435:467	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	77	from	receptors	330:338	arg1	cells					362:366	antigen-presenting cells	343:366	antigen-presenting cells	343:366	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	3	78	link	N-linked	575:582	arg1	oligosaccharides					584:599	N-linked oligosaccharides	575:599	N-linked oligosaccharides	575:599	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	5	79	theme	enzyme-catalyzed	984:999	arg1	N-deglycosylation					1001:1017	enzyme-catalyzed N-deglycosylation	984:1017	enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH	984:1038	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	2	80	theme	proinflammatory	518:532	arg1	secretion					543:551	proinflammatory cytokine secretion	518:551	proinflammatory cytokine secretion	518:551	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	8	81	theme	N-deglycosylated	1597:1612	arg1	hemocyanins					1614:1624	N-deglycosylated hemocyanins	1597:1624	N-deglycosylated hemocyanins	1597:1624	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	6	82	theme	Biochemical	1115:1125	arg1	analyses					1127:1134	Biochemical analyses	1115:1134	Biochemical analyses	1115:1134	Biochemical analyses revealed that the deglycosylation does not change hemocyanin secondary structure but alters their refolding mechanism and dodecameric structure.					
31719148	4	83	theme	Megathura	770:778	arg1	KLH					791:793	KLH	791:793	KLH	791:793	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	4	83	theme	Megathura	770:778	arg1	crenulata					780:788	Megathura crenulata	770:788	Megathura crenulata (KLH)	770:794	Mollusk hemocyanins, such as Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), are oligomeric glycoproteins with complex dodecameric quaternary structures and heterogeneous glycosylation patterns, primarily consisting of mannose-rich N-glycans.					
31719148	8	84	from	function	1585:1592	arg1	effects					1689:1695	their nonspecific antitumor effects	1661:1695	their nonspecific antitumor effects	1661:1695	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	8	84	from	function	1585:1592	arg1	response					1648:1655	the humoral immune response	1629:1655	the humoral immune response	1629:1655	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	7	85	from	production	1455:1464	arg1	macrophages					1557:1567	macrophages	1557:1567	macrophages	1557:1567	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	86	theme	humoral	1633:1639	arg1	response					1648:1655	the humoral immune response	1629:1655	the humoral immune response	1629:1655	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	2	87	theme	mannose	412:418	arg1	MR					430:431	MR	430:431	MR	430:431	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	87	theme	mannose	412:418	arg1	receptor					420:427	mannose receptor	412:427	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	0	88	theme	hemocyanins	27:37	arg1	N-Glycosylation					0:14	N-Glycosylation	0:14	N-Glycosylation of mollusk hemocyanins	0:37	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	2	89	theme	macrophage	435:444	arg1	MGL					464:466	MGL	464:466	MGL	464:466	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	2	89	theme	macrophage	435:444	arg1	lectin					456:461	macrophage galactose lectin	435:461	macrophage galactose lectin (MGL)	435:467	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
31719148	5	90	theme	KLH	1036:1038	arg1	N-deglycosylation					1001:1017	enzyme-catalyzed N-deglycosylation	984:1017	enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH	984:1038	Here, we report that enzyme-catalyzed N-deglycosylation of CCH, FLH, and KLH disrupts their quaternary structure and impairs their immunogenic effects.					
31719148	7	91	theme	MGL	1379:1381	arg1	receptors					1383:1391	the MR and MGL receptors	1368:1391	receptors	1383:1391	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	9	92	theme	glycan	1938:1943	arg1	content					1945:1951	the glycan content	1934:1951	the glycan content of hemocyanins	1934:1966	In conclusion, the glycan content of hemocyanins is, among other structural characteristics, critically required for their immunological activities and should be considered in biomedical applications.					
31719148	3	93	theme	hemocyanin	651:660	arg1	properties					637:646	the structural and immunological properties	604:646	the structural and immunological properties of hemocyanin	604:660	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	0	94	theme	structural	60:69	arg1	stability					71:79	their structural stability	54:79	their structural stability	54:79	N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals.					
31719148	7	95	theme	MR	1372:1373	arg1	receptors					1383:1391	the MR and MGL receptors	1368:1391	receptors	1383:1391	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	96	theme	antitumor	1679:1687	arg1	effects					1689:1695	their nonspecific antitumor effects	1661:1695	their nonspecific antitumor effects	1661:1695	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	3	97	theme	structural	608:617	arg1	properties					637:646	the structural and immunological properties	604:646	the structural and immunological properties of hemocyanin	604:660	However, the role of N-linked oligosaccharides on the structural and immunological properties of hemocyanin is unclear.					
31719148	7	98	theme	N-deglycosylated	1336:1351	arg1	hemocyanins					1353:1363	N-deglycosylated hemocyanins	1336:1363	N-deglycosylated hemocyanins	1336:1363	Immunochemical analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors and TLR4 and reduced endocytosis concomitant with an impaired production of tumor necrosis factor α, and interleukins 6 and 12 (IL-6 and IL-12p40, respectively) in macrophages.					
31719148	8	99	theme	B16F10	1704:1709	arg1	model					1720:1724	the B16F10 melanoma model	1700:1724	the B16F10 melanoma model	1700:1724	Evaluating the function of N-deglycosylated hemocyanins in the humoral immune response and their nonspecific antitumor effects in the B16F10 melanoma model, we found that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody titers, as well as partially diminished antitumor effects and altered carrier activities.					
31719148	2	100	theme	immune	395:400	arg1	receptors					402:410	the C-type lectin immune receptors	377:410	the C-type lectin immune receptors mannose receptor (MR)	377:432	Hemocyanins also interact with glycan-recognizing innate immune receptors on antigen-presenting cells, such as the C-type lectin immune receptors mannose receptor (MR), macrophage galactose lectin (MGL), and the Toll-like receptors (TLRs), stimulating proinflammatory cytokine secretion.					
29775069	0	0	theme	Glycomic	73:80	arg1	Analysis					82:89	Rapid Glycomic Analysis	67:89	Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples	67:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	5	1	theme	spectrometric	1241:1253	arg1	detection					1255:1263	high-sensitivity spectrometric detection	1224:1263	high-sensitivity spectrometric detection	1224:1263	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	5	2	theme	high-resolution	1177:1191	arg1	separation					1209:1218	high-resolution chromatographic separation	1177:1218	high-resolution chromatographic separation	1177:1218	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	0	3	theme	Rapid	67:71	arg1	Analysis					82:89	Rapid Glycomic Analysis	67:89	Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples	67:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	4	4	theme	Knoevenagel	927:937	arg1	condensation					939:950	Knoevenagel condensation	927:950	Knoevenagel condensation	927:950	The released glycans are promptly derivatized with PMP in situ by Knoevenagel condensation and Michael addition, with peeling degradation almost completely prevented.					
29775069	1	5	theme	glycosylation	372:384	arg1	forms					386:390	the glycosylation forms	368:390	the glycosylation forms	368:390	Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed.					
29775069	4	6	theme	Michael	956:962	arg1	addition					964:971	Michael addition	956:971	Michael addition	956:971	The released glycans are promptly derivatized with PMP in situ by Knoevenagel condensation and Michael addition, with peeling degradation almost completely prevented.					
29775069	0	7	theme	structures	238:247	arg1	characterization					212:227	characterization	212:227	characterization of their structures very complicated and time-consuming	212:283	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	8	dep	LC-UV-ESI-MS/MS.	101:116	arg1	glycoproteins					123:135	Most glycoproteins	118:135	Most glycoproteins	118:135	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	6	9	theme	N-glycan	1610:1617	arg1	structures					1619:1628	some novel O- and N-glycan structures	1592:1628	structures	1619:1628	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	7	10	theme	glycomics	1718:1726	arg1	analysis					1728:1735	high-throughput glycomics analysis	1702:1735	high-throughput glycomics analysis	1702:1735	This method provides a simple, versatile strategy for high-throughput glycomics analysis.					
29775069	3	11	theme	protein	778:784	arg1	backbones					786:794	protein backbones	778:794	protein backbones	778:794	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	6	12	theme	significant	1404:1414	arg1	peeling					1416:1422	significant peeling	1404:1422	significant peeling	1404:1422	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	5	13	theme	O-	1053:1054	arg1	mixture					1042:1048	The recovered mixture	1028:1048	The recovered mixture of O- and N-glycans as bis-PMP derivatives	1028:1091	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	5	14	theme	chromatographic	1193:1207	arg1	separation					1209:1218	high-resolution chromatographic separation	1177:1218	high-resolution chromatographic separation	1177:1218	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	0	15	theme	complicated	254:264	arg1	structures					238:247	their structures	232:247	their structures very complicated and time-consuming	232:283	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	2	16	gly	glycoproteins	528:540	arg1	glycoproteins					528:540	glycoproteins	528:540	glycoproteins	528:540	Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization.					
29775069	3	17	gly	glycoproteins	646:658	arg1	glycoproteins					646:658	glycoproteins	646:658	glycoproteins	646:658	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	6	18	theme	online	1529:1534	arg1	HILIC-UV-ESI-MS/MS					1536:1553	online HILIC-UV-ESI-MS/MS	1529:1553	online HILIC-UV-ESI-MS/MS	1529:1553	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	6	19	gly	glycoproteins	1350:1362	arg1	glycoproteins					1350:1362	model glycoproteins	1344:1362	model glycoproteins	1344:1362	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	5	20	theme	high-sensitivity	1224:1239	arg1	detection					1255:1263	high-sensitivity spectrometric detection	1224:1263	high-sensitivity spectrometric detection	1224:1263	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	5	21	theme	N-glycans	1060:1068	arg1	mixture					1042:1048	The recovered mixture	1028:1048	The recovered mixture of O- and N-glycans as bis-PMP derivatives	1028:1091	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	3	22	attach	released	764:771	arg2	O-glycans					754:762	O-glycans	754:762	O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis	754:858	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	3	22	attach	released	764:771	arg1	backbones					786:794	protein backbones	778:794	protein backbones	778:794	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	0	23	theme	time-consuming	270:283	arg1	structures					238:247	their structures	232:247	their structures very complicated and time-consuming	232:283	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	24	theme	protein	152:158	arg1	samples					160:166	biological protein samples	141:166	biological protein samples	141:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	25	gly	N-glycosylation	188:202	arg1	Labeling					25:32	Labeling	25:32	Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples	25:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	25	gly	N-glycosylation	188:202	arg1	Release					13:19	Simultaneous Release	0:19	Simultaneous Release	0:19	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	26	theme	Simultaneous	0:11	arg1	Release					13:19	Simultaneous Release	0:19	Simultaneous Release	0:19	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	6	27	theme	biological	1376:1385	arg1	samples					1387:1393	complex biological samples	1368:1393	complex biological samples	1368:1393	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	7	28	theme	simple	1671:1676	arg1	strategy					1689:1696	a simple, versatile strategy	1669:1696	a simple, versatile strategy for high-throughput glycomics analysis	1669:1735	This method provides a simple, versatile strategy for high-throughput glycomics analysis.					
29775069	2	29	theme	one-pot	454:460	arg1	method					462:467	a versatile, convenient one-pot method	430:467	a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization	430:624	Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization.					
29775069	6	30	theme	model	1344:1348	arg1	glycoproteins					1350:1362	model glycoproteins	1344:1362	model glycoproteins	1344:1362	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	5	31	theme	recovered	1032:1040	arg1	mixture					1042:1048	The recovered mixture	1028:1048	The recovered mixture of O- and N-glycans as bis-PMP derivatives	1028:1091	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	0	32	theme	Most	118:121	arg1	glycoproteins					123:135	Most glycoproteins	118:135	Most glycoproteins	118:135	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	2	33	theme	versatile	432:440	arg1	method					462:467	a versatile, convenient one-pot method	430:467	a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization	430:624	Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization.					
29775069	0	34	theme	O-	37:38	arg1	Labeling					25:32	Labeling	25:32	Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples	25:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	34	theme	O-	37:38	arg1	Release					13:19	Simultaneous Release	0:19	Simultaneous Release	0:19	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	35	theme	biological	141:150	arg1	samples					160:166	biological protein samples	141:166	biological protein samples	141:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	7	36	theme	high-throughput	1702:1716	arg1	analysis					1728:1735	high-throughput glycomics analysis	1702:1735	high-throughput glycomics analysis	1702:1735	This method provides a simple, versatile strategy for high-throughput glycomics analysis.					
29775069	6	37	theme	complex	1368:1374	arg1	samples					1387:1393	complex biological samples	1368:1393	complex biological samples	1368:1393	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	1	38	theme	biological	324:333	arg1	functions					335:343	the biological functions	320:343	the biological functions of glycosylation	320:360	Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed.					
29775069	6	39	theme	other	1470:1474	arg1	side-reactions					1476:1489	other side-reactions	1470:1489	other side-reactions	1470:1489	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	6	40	theme	novel	1597:1601	arg1	O-					1603:1604	some novel O- and N-glycan structures	1592:1628	O-	1603:1604	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	0	41	theme	N-Glycans	44:52	arg1	Labeling					25:32	Labeling	25:32	Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples	25:166	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	0	41	theme	N-Glycans	44:52	arg1	Release					13:19	Simultaneous Release	0:19	Simultaneous Release	0:19	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	3	42	theme	alkaline	840:847	arg1	hydrolysis					849:858	alkaline hydrolysis	840:858	alkaline hydrolysis	840:858	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	5	43	theme	absorbing	1126:1134	arg1	ability					1136:1142	ability	1136:1142	ability	1136:1142	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	2	44	theme	further	601:607	arg1	characterization					609:624	further characterization	601:624	further characterization	601:624	Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization.					
29775069	3	45	theme	aqueous	719:725	arg1	hydroxide					736:744	aqueous ammonium hydroxide	719:744	aqueous ammonium hydroxide	719:744	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	2	46	dep	versatile	432:440	arg1	convenient					443:452	convenient	443:452	convenient	443:452	Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labeled in situ and thus available for further characterization.					
29775069	0	47	gly	glycoproteins	123:135	arg1	glycoproteins					123:135	Most glycoproteins	118:135	Most glycoproteins	118:135	Simultaneous Release and Labeling of O- and N-Glycans Allowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming.					
29775069	1	48	theme	glycosylation	348:360	arg1	functions					335:343	the biological functions	320:343	the biological functions of glycosylation	320:360	Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed.					
29775069	4	49	theme	peeling	979:985	arg1	degradation					987:997	peeling degradation	979:997	peeling degradation	979:997	The released glycans are promptly derivatized with PMP in situ by Knoevenagel condensation and Michael addition, with peeling degradation almost completely prevented.					
29775069	3	50	theme	ammonium	727:734	arg1	hydroxide					736:744	aqueous ammonium hydroxide	719:744	aqueous ammonium hydroxide	719:744	In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by β-elimination and N-glycans liberated by alkaline hydrolysis.					
29775069	5	51	theme	bis-PMP	1073:1079	arg1	derivatives					1081:1091	bis-PMP derivatives	1073:1091	bis-PMP derivatives	1073:1091	The recovered mixture of O- and N-glycans as bis-PMP derivatives features strong ultraviolet (UV) absorbing ability and hydrophobicity, allowing for high-resolution chromatographic separation and high-sensitivity spectrometric detection.					
29775069	6	52	dep	glycoproteins	1350:1362	arg1	some					1339:1342	some	1339:1342	some	1339:1342	Using this technique, O- and N-glycans were simultaneously prepared from some model glycoproteins and complex biological samples, without significant peeling, desialylation, deacetylation, desulfation or other side-reactions, and then comprehensively analyzed by online HILIC-UV-ESI-MS/MS and RP-HPLC-UV-ESI-MS/MS, with which some novel O- and N-glycan structures were first found.					
29775069	7	53	dep	simple	1671:1676	arg1	versatile					1679:1687	versatile	1679:1687	versatile	1679:1687	This method provides a simple, versatile strategy for high-throughput glycomics analysis.					
29775069	1	54	dep	understand	309:318	arg1	both					363:366	both	363:366	both	363:366	Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed.					
29775069	4	55	theme	released	865:872	arg1	glycans					874:880	The released glycans	861:880	The released glycans	861:880	The released glycans are promptly derivatized with PMP in situ by Knoevenagel condensation and Michael addition, with peeling degradation almost completely prevented.					
31809011	8	0	theme	relative	1222:1229	arg1	intensity					1231:1239	the relative intensity	1218:1239	the relative intensity of the six states of N-glycan type glycosylation on each site	1218:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	1	gly	glycoproteins	1356:1368	arg1	glycoproteins					1356:1368	glycoproteins	1356:1368	glycoproteins	1356:1368	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	2	theme	quantitative	815:826	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	2	theme	quantitative	815:826	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	3	theme	glycoproteins	1356:1368	arg1	function					1344:1351	the function	1340:1351	the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development	1340:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	4	theme	novel	1178:1182	arg1	strategy					1197:1204	The novel quantitative strategy	1174:1204	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site	1174:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	5	theme	type	1271:1274	arg1	glycosylation					1276:1288	N-glycan type glycosylation	1262:1288	N-glycan type glycosylation	1262:1288	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	6	used	used	856:859	arg2	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	6	used	used	856:859	arg2	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	7	from	intensity	1231:1239	arg1	site					1298:1301	each site	1293:1301	each site	1293:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	7	8	theme	breast	1032:1037	arg1	line					1051:1054	breast cancer cell line	1032:1054	the breast cancer cell line MCF7	1028:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	8	9	theme	biomarker	1401:1409	arg1	research					1411:1418	cancer biomarker research	1394:1418	cancer biomarker research	1394:1418	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	7	10	theme	cell	1046:1049	arg1	line					1051:1054	breast cancer cell line	1032:1054	the breast cancer cell line MCF7	1028:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	8	11	theme	broad	1373:1377	arg1	research					1411:1418	cancer biomarker research	1394:1418	cancer biomarker research	1394:1418	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	11	theme	broad	1373:1377	arg1	characterization					1437:1452	pharmaceuticals characterization	1421:1452	pharmaceuticals characterization	1421:1452	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	11	theme	broad	1373:1377	arg1	development					1478:1488	antiglycan vaccine development	1459:1488	antiglycan vaccine development	1459:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	11	theme	broad	1373:1377	arg1	areas					1379:1383	broad areas	1373:1383	broad areas	1373:1383	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	3	12	gly	glycoproteins	329:341	arg1	glycoproteins					329:341	glycoproteins	329:341	glycoproteins	329:341	However, the systematic quantitation of glycoproteins has remained largely unexplored.					
31809011	7	13	from	line	1051:1054	arg1	profile					1003:1009	the glycan type profile	987:1009	the glycan type profile of proteins from the breast cancer cell line MCF7	987:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	7	13	from	line	1051:1054	arg1	proteins					1014:1021	proteins	1014:1021	proteins from the breast cancer cell line MCF7	1014:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	8	14	theme	cancer	1394:1399	arg1	research					1411:1418	cancer biomarker research	1394:1418	cancer biomarker research	1394:1418	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	5	15	theme	triantennary	701:712	arg1	fucose					732:737	biantennary, and triantennary with/without core fucose	684:737	biantennary, and triantennary with/without core fucose	684:737	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	15	theme	triantennary	701:712	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	7	16	dep	line	1051:1054	arg1	MCF7					1056:1059	MCF7	1056:1059	the breast cancer cell line MCF7	1028:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	5	17	theme	labeling	581:588	arg1	specificity					518:528	the specificity	514:528	the specificity of different endoglycosidases and isotope dimethyl labeling	514:588	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	18	attach	linked	624:629	arg3	glycopeptide					639:650	each glycopeptide	634:650	each glycopeptide	634:650	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	18	attach	linked	624:629	arg2	structures					613:622	structures	613:622	structures linked on each glycopeptide	613:650	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	2	19	theme	glycoproteome	176:188	arg1	methods					190:196	Current glycoproteome methods	168:196	Current glycoproteome methods	168:196	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	5	20	theme	core	727:730	arg1	fucose					732:737	biantennary, and triantennary with/without core fucose	684:737	biantennary, and triantennary with/without core fucose	684:737	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	20	theme	core	727:730	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	4	21	theme	N-glycan	478:485	arg1	types					487:491	the N-glycan types	474:491	the N-glycan types	474:491	Here, we developed a chemoenzymatic method to quantitatively investigate N-glycoproteome based on the N-glycan types.					
31809011	6	22	theme	N-glycan	801:808	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	22	theme	N-glycan	801:808	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	2	23	theme	Current	168:174	arg1	methods					190:196	Current glycoproteome methods	168:196	Current glycoproteome methods	168:196	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	0	24	theme	Type	50:53	arg1	Quantitation					55:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation.					
31809011	6	25	theme	N-glycan	878:885	arg1	composition					892:902	the N-glycan type composition	874:902	the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment	874:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	26	theme	states	1252:1257	arg1	intensity					1231:1239	the relative intensity	1218:1239	the relative intensity of the six states of N-glycan type glycosylation on each site	1218:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	27	theme	glycoproteomic	786:799	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	27	theme	glycoproteomic	786:799	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	7	28	theme	proteins	1014:1021	arg1	profile					1003:1009	the glycan type profile	987:1009	the glycan type profile of proteins from the breast cancer cell line MCF7	987:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	7	29	theme	type	998:1001	arg1	profile					1003:1009	the glycan type profile	987:1009	the glycan type profile of proteins from the breast cancer cell line MCF7	987:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	5	30	theme	N-glycan	595:602	arg1	types					604:608	six N-glycan types	591:608	six N-glycan types	591:608	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	30	theme	N-glycan	595:602	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	7	31	gly	glycoprotein	1154:1165	arg1	glycoprotein					1154:1165	the glycopeptide and glycoprotein level	1133:1171	glycoprotein	1154:1165	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	0	32	theme	Chemoenzymatic	0:13	arg1	Method					15:20	Chemoenzymatic Method	0:20	Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation.	0:67	Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation.					
31809011	7	33	theme	glycoprotein	1154:1165	arg1	level					1167:1171	the glycopeptide and glycoprotein level	1133:1171	level	1167:1171	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	1	34	from	modifications	131:143	arg1	systems					159:165	biological systems	148:165	biological systems	148:165	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	8	35	theme	pharmaceuticals	1421:1435	arg1	characterization					1437:1452	pharmaceuticals characterization	1421:1452	pharmaceuticals characterization	1421:1452	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	5	36	theme	endoglycosidases	543:558	arg1	specificity					518:528	the specificity	514:528	the specificity of different endoglycosidases and isotope dimethyl labeling	514:588	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	8	37	theme	new	1314:1316	arg1	avenue					1318:1323	a new avenue	1312:1323	a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development	1312:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	0	38	theme	Glycoproteomic	26:39	arg1	Quantitation					55:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation.					
31809011	7	39	theme	glycan	991:996	arg1	profile					1003:1009	the glycan type profile	987:1009	the glycan type profile of proteins from the breast cancer cell line MCF7	987:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	3	40	theme	glycoproteins	329:341	arg1	quantitation					313:324	the systematic quantitation	298:324	the systematic quantitation of glycoproteins	298:341	However, the systematic quantitation of glycoproteins has remained largely unexplored.					
31809011	5	41	theme	with/without	714:725	arg1	fucose					732:737	biantennary, and triantennary with/without core fucose	684:737	biantennary, and triantennary with/without core fucose	684:737	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	41	theme	with/without	714:725	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	1	42	theme	important	102:110	arg1	modifications					131:143	the most important post-translational modifications	93:143	the most important post-translational modifications in biological systems	93:165	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	6	43	theme	principle	771:779	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	43	theme	principle	771:779	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	44	theme	N-glycan	1262:1269	arg1	glycosylation					1276:1288	N-glycan type glycosylation	1262:1288	N-glycan type glycosylation	1262:1288	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	7	45	theme	glycopeptide	1137:1148	arg1	level					1167:1171	the glycopeptide and glycoprotein level	1133:1171	level	1167:1171	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	2	46	theme	qualitative	214:224	arg1	identification					226:239	qualitative identification	214:239	qualitative identification of glycosylation sites or intact glycopeptides	214:286	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	1	47	theme	post-translational	112:129	arg1	modifications					131:143	the most important post-translational modifications	93:143	the most important post-translational modifications in biological systems	93:165	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	5	48	theme	isotope	564:570	arg1	labeling					581:588	isotope dimethyl labeling	564:588	isotope dimethyl labeling	564:588	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	2	49	gly	glycopeptides	274:286	arg2	glycopeptides					274:286	intact glycopeptides	267:286	intact glycopeptides	267:286	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	5	50	gly	glycopeptide	639:650	arg2	glycopeptide					639:650	each glycopeptide	634:650	each glycopeptide	634:650	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	6	51	theme	fragment	939:946	arg1	composition					892:902	the N-glycan type composition	874:902	the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment	874:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	52	theme	vaccine	1470:1476	arg1	development					1478:1488	antiglycan vaccine development	1459:1488	antiglycan vaccine development	1459:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	5	53	theme	dimethyl	572:579	arg1	labeling					581:588	isotope dimethyl labeling	564:588	isotope dimethyl labeling	564:588	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	0	54	theme	N-Glycan	41:48	arg1	Quantitation					55:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Glycoproteomic N-Glycan Type Quantitation	26:66	Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation.					
31809011	7	55	theme	cancer	1039:1044	arg1	line					1051:1054	breast cancer cell line	1032:1054	the breast cancer cell line MCF7	1028:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	6	56	theme	Fc	936:937	arg1	fragment					939:946	the immunoglobulin G1 (IgG1) Fc fragment	907:946	the immunoglobulin G1 (IgG1) Fc fragment	907:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	2	57	theme	glycopeptides	274:286	arg1	identification					226:239	qualitative identification	214:239	qualitative identification of glycosylation sites or intact glycopeptides	214:286	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	4	58	theme	chemoenzymatic	397:410	arg1	method					412:417	a chemoenzymatic method	395:417	a chemoenzymatic method to quantitatively investigate N-glycoproteome based on the N-glycan types	395:491	Here, we developed a chemoenzymatic method to quantitatively investigate N-glycoproteome based on the N-glycan types.					
31809011	7	59	theme	N-glycan	1097:1104	arg1	microheterogeneity					1111:1128	the N-glycan type microheterogeneity	1093:1128	the N-glycan type microheterogeneity	1093:1128	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	2	60	theme	intact	267:272	arg1	glycopeptides					274:286	intact glycopeptides	267:286	intact glycopeptides	267:286	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	1	61	theme	modifications	131:143	arg1	one					86:88	one	86:88	one	86:88	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	1	61	theme	modifications	131:143	arg1	modifications					131:143	the most important post-translational modifications	93:143	the most important post-translational modifications in biological systems	93:165	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	7	62	theme	type	1106:1109	arg1	microheterogeneity					1111:1128	the N-glycan type microheterogeneity	1093:1128	the N-glycan type microheterogeneity	1093:1128	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	2	63	theme	sites	258:262	arg1	identification					226:239	qualitative identification	214:239	qualitative identification of glycosylation sites or intact glycopeptides	214:286	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	3	64	theme	systematic	302:311	arg1	quantitation					313:324	the systematic quantitation	298:324	the systematic quantitation of glycoproteins	298:341	However, the systematic quantitation of glycoproteins has remained largely unexplored.					
31809011	8	65	theme	glycosylation	1276:1288	arg1	states					1252:1257	the six states	1244:1257	the six states of N-glycan type glycosylation	1244:1288	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	2	66	gly	glycosylation	244:256	arg2	sites					258:262	glycosylation sites	244:262	glycosylation sites	244:262	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	5	67	theme	biantennary	684:694	arg1	fucose					732:737	biantennary, and triantennary with/without core fucose	684:737	biantennary, and triantennary with/without core fucose	684:737	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	67	theme	biantennary	684:694	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	2	68	theme	glycosylation	244:256	arg1	sites					258:262	glycosylation sites	244:262	glycosylation sites	244:262	Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides.					
31809011	8	69	theme	antiglycan	1459:1468	arg1	development					1478:1488	antiglycan vaccine development	1459:1488	antiglycan vaccine development	1459:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	1	70	theme	biological	148:157	arg1	systems					159:165	biological systems	148:165	biological systems	148:165	Glycosylation is one of the most important post-translational modifications in biological systems.					
31809011	5	71	theme	specificity	518:528	arg1	advantage					501:509	advantage	501:509	advantage of the specificity of different endoglycosidases and isotope dimethyl labeling	501:588	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	8	72	theme	quantitative	1184:1195	arg1	strategy					1197:1204	The novel quantitative strategy	1174:1204	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site	1174:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	73	theme	G1	926:927	arg1	fragment					939:946	the immunoglobulin G1 (IgG1) Fc fragment	907:946	the immunoglobulin G1 (IgG1) Fc fragment	907:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	8	74	from	function	1344:1351	arg1	research					1411:1418	cancer biomarker research	1394:1418	cancer biomarker research	1394:1418	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	74	from	function	1344:1351	arg1	characterization					1437:1452	pharmaceuticals characterization	1421:1452	pharmaceuticals characterization	1421:1452	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	74	from	function	1344:1351	arg1	development					1478:1488	antiglycan vaccine development	1459:1488	antiglycan vaccine development	1459:1488	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	8	74	from	function	1344:1351	arg1	areas					1379:1383	broad areas	1373:1383	broad areas	1373:1383	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	75	theme	type	887:890	arg1	composition					892:902	the N-glycan type composition	874:902	the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment	874:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	7	76	from	profile	1003:1009	arg1	line					1051:1054	breast cancer cell line	1032:1054	the breast cancer cell line MCF7	1028:1059	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	6	77	theme	immunoglobulin	911:924	arg1	IgG1					930:933	IgG1	930:933	IgG1	930:933	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	77	theme	immunoglobulin	911:924	arg1	G1					926:927	immunoglobulin G1	911:927	the immunoglobulin G1 (IgG1) Fc fragment	907:946	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	78	theme	glyco-TQ	829:836	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	78	theme	glyco-TQ	829:836	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	7	79	gly	glycopeptide	1137:1148	arg2	glycopeptide					1137:1148	the glycopeptide and glycoprotein level	1133:1171	glycopeptide	1137:1148	Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level.					
31809011	5	80	theme	different	533:541	arg1	endoglycosidases					543:558	different endoglycosidases	533:558	different endoglycosidases	533:558	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	81	theme	structures	613:622	arg1	types					604:608	six N-glycan types	591:608	six N-glycan types	591:608	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	5	81	theme	structures	613:622	arg1	high-mannose/hybrid					663:681	high-mannose/hybrid	663:681	high-mannose/hybrid	663:681	Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified.					
31809011	8	82	dep	strategy	1197:1204	arg1	evaluate					1209:1216	evaluate	1209:1216	to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site	1206:1301	The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.					
31809011	6	83	theme	type	810:813	arg1	proof					762:766	a proof	760:766	a proof of principle	760:779	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
31809011	6	83	theme	type	810:813	arg1	method					839:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	the glycoproteomic N-glycan type quantitative (glyco-TQ) method	782:844	As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment.					
29936827	5	0	gly	glycoproteins	895:907	arg1	glycoproteins					895:907	glycoproteins	895:907	glycoproteins	895:907	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	7	1	theme	O-glycosylated	1164:1177	arg1	peptides					1179:1186	O-glycosylated peptides	1164:1186	O-glycosylated peptides	1164:1186	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	10	2	from	set	1779:1781	arg1	antigens					1834:1841	these important viral antigens	1812:1841	these important viral antigens	1812:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	9	3	theme	high	1496:1499	arg1	accuracy					1501:1508	high accuracy	1496:1508	high accuracy	1496:1508	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	5	4	theme	O-glycans	937:945	arg1	types					928:932	other types	922:932	other types of O-glycans	922:945	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	8	5	theme	first	1293:1297	arg1	time					1299:1302	the first time	1289:1302	the first time	1289:1302	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	4	6	theme	due	704:706	arg1	challenge					694:702	a challenge	692:702	a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans	692:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	2	7	gly	glycosylated	272:283	arg1	%					225:225	Over 50%	218:225	Over 50% of mammalian cellular proteins	218:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	7	gly	glycosylated	272:283	arg1	proteins					249:256	mammalian cellular proteins	230:256	mammalian cellular proteins	230:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	3	8	theme	enzymes	591:597	arg1	range					573:577	a range	571:577	a range of specific enzymes available for their release	571:625	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	1	9	theme	Golgi	201:205	arg1	apparatus					207:215	Golgi apparatus	201:215	Golgi apparatus	201:215	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	7	10	gly	O-glycosylated	1164:1177	arg1	peptides					1179:1186	O-glycosylated peptides	1164:1186	O-glycosylated peptides	1164:1186	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	8	11	theme	intact	1320:1325	arg1	O-glycopeptides					1327:1341	intact O-glycopeptides	1320:1341	intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform	1320:1407	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	10	12	theme	glycoproteins	1706:1718	arg1	analysis					1671:1678	analysis	1671:1678	analysis of Zika virus recombinant glycoproteins	1671:1718	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	11	13	theme	O-glycosylation	1915:1929	arg1	investigation					1890:1902	the investigation	1886:1902	the investigation of protein O-glycosylation in diseases and other biomedical contexts	1886:1971	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	2	14	theme	mammalian	230:238	arg1	proteins					249:256	mammalian cellular proteins	230:256	mammalian cellular proteins	230:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	9	15	dep	glycoproteins	1446:1458	arg1	confirming					1461:1470	confirming	1461:1470	confirming known O-glycosites with high accuracy and confidence	1461:1523	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	10	16	theme	tracts	1802:1807	arg1	set					1779:1781	a basis set	1771:1781	a basis set of O-glycosylation tracts in these important viral antigens	1771:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	10	16	theme	tracts	1802:1807	arg1	tracts					1802:1807	O-glycosylation tracts	1786:1807	O-glycosylation tracts in these important viral antigens	1786:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	4	17	theme	enzymes	734:740	arg1	lack					715:718	the lack	711:718	the lack of equivalent enzymes	711:740	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	4	17	theme	enzymes	734:740	arg1	heterogeneity					770:782	the inherent structural heterogeneity	746:782	the inherent structural heterogeneity of O-glycans	746:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	10	18	theme	virus	1688:1692	arg1	glycoproteins					1706:1718	Zika virus recombinant glycoproteins	1683:1718	Zika virus recombinant glycoproteins	1683:1718	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	1	19	theme	biosynthetic-secretory	132:153	arg1	pathway					155:161	the biosynthetic-secretory pathway	128:161	the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus	128:215	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	3	20	link	N-linked	501:508	arg1	analysis					524:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	8	21	gly	O-glycopeptides	1327:1341	arg2	O-glycopeptides					1327:1341	intact O-glycopeptides	1320:1341	intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform	1320:1407	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	2	22	theme	proteins	249:256	arg1	%					225:225	Over 50%	218:225	Over 50% of mammalian cellular proteins	218:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	22	theme	proteins	249:256	arg1	proteins					249:256	mammalian cellular proteins	230:256	mammalian cellular proteins	230:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	5	23	theme	other	922:926	arg1	types					928:932	other types	922:932	other types of O-glycans	922:945	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	6	24	theme	chemoenzymatic	1037:1050	arg1	approach					1052:1059	a novel chemoenzymatic approach	1029:1059	a novel chemoenzymatic approach driven by a newly available O-protease	1029:1098	Here, we describe a novel chemoenzymatic approach driven by a newly available O-protease and solid phase platform.					
29936827	7	25	theme	O-glycosylation	1268:1282	arg1	mapping					1243:1249	mapping	1243:1249	mapping of heterogeneous O-glycosylation	1243:1282	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	25	theme	O-glycosylation	1268:1282	arg1	assignment					1150:1159	the assignment	1146:1159	the assignment of O-glycosylated peptides	1146:1186	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	25	theme	O-glycosylation	1268:1282	arg1	profile					1198:1204	N-glycan profile	1189:1204	N-glycan profile	1189:1204	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	25	theme	O-glycosylation	1268:1282	arg1	linkage					1230:1236	sialyl O-glycopeptides linkage	1207:1236	sialyl O-glycopeptides linkage	1207:1236	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	9	26	theme	concomitant	1596:1606	arg1	heterogeneity					1618:1630	concomitant increased heterogeneity	1596:1630	concomitant increased heterogeneity	1596:1630	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	27	gly	glycosites	1555:1564	arg2	glycosites					1555:1564	up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity	1537:1630	up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity	1537:1630	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	28	dep	8-fold	1543:1548	arg1	more					1550:1553	more	1550:1553	more	1550:1553	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	28	dep	8-fold	1543:1548	arg1	to					1540:1541	to	1540:1541	to	1540:1541	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	0	29	theme	Chemoenzymatic	49:62	arg1	Cleavage					64:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment.					
29936827	1	30	from	pathway	155:161	arg1	ER					193:194	ER	193:194	ER	193:194	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	1	30	from	pathway	155:161	arg1	reticulum					182:190	the endoplasmic reticulum	166:190	the endoplasmic reticulum (ER)	166:195	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	1	30	from	pathway	155:161	arg1	apparatus					207:215	Golgi apparatus	201:215	Golgi apparatus	201:215	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	6	31	theme	phase	1110:1114	arg1	platform					1116:1123	solid phase platform	1104:1123	solid phase platform	1104:1123	Here, we describe a novel chemoenzymatic approach driven by a newly available O-protease and solid phase platform.					
29936827	4	32	theme	structural	759:768	arg1	heterogeneity					770:782	the inherent structural heterogeneity	746:782	the inherent structural heterogeneity of O-glycans	746:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	2	33	theme	intestinal	390:399	arg1	microbes					401:408	intestinal microbes	390:408	intestinal microbes	390:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	5	34	theme	core	849:852	arg1	removal					838:844	the removal	834:844	the removal of core 1 and core 3 O-linked disaccharides from glycoproteins	834:907	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	1	35	theme	critical	111:118	arg1	role					120:123	a critical role	109:123	a critical role	109:123	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	10	36	theme	basis	1773:1777	arg1	set					1779:1781	a basis set	1771:1781	a basis set of O-glycosylation tracts in these important viral antigens	1771:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	10	36	theme	basis	1773:1777	arg1	tracts					1802:1807	O-glycosylation tracts	1786:1807	O-glycosylation tracts in these important viral antigens	1786:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	7	37	theme	sialyl	1207:1212	arg1	linkage					1230:1236	sialyl O-glycopeptides linkage	1207:1236	sialyl O-glycopeptides linkage	1207:1236	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	6	38	theme	available	1079:1087	arg1	O-protease					1089:1098	a newly available O-protease	1071:1098	a newly available O-protease	1071:1098	Here, we describe a novel chemoenzymatic approach driven by a newly available O-protease and solid phase platform.					
29936827	2	39	theme	immune	486:491	arg1	system					493:498	the innate immune system	475:498	the innate immune system	475:498	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	9	40	gly	glycoproteins	1446:1458	arg1	glycoproteins					1446:1458	standard glycoproteins	1437:1458	standard glycoproteins	1437:1458	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	0	41	theme	Solid-Phase	37:47	arg1	Cleavage					64:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment.					
29936827	3	42	theme	N-linked	501:508	arg1	analysis					524:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	9	43	gly	O-glycosites	1478:1489	arg2	O-glycosites					1478:1489	known O-glycosites	1472:1489	known O-glycosites with high accuracy and confidence	1472:1523	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	44	with	O-glycosites	1478:1489	arg1	confidence					1514:1523	confidence	1514:1523	confidence	1514:1523	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	44	with	O-glycosites	1478:1489	arg1	accuracy					1501:1508	high accuracy	1496:1508	high accuracy	1496:1508	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	45	theme	standard	1437:1444	arg1	glycoproteins					1446:1458	standard glycoproteins	1437:1458	standard glycoproteins	1437:1458	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	1	46	theme	endoplasmic	170:180	arg1	ER					193:194	ER	193:194	ER	193:194	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	1	46	theme	endoplasmic	170:180	arg1	reticulum					182:190	the endoplasmic reticulum	166:190	the endoplasmic reticulum (ER)	166:195	Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus.					
29936827	10	47	theme	O-glycosylation	1786:1800	arg1	tracts					1802:1807	O-glycosylation tracts	1786:1807	O-glycosylation tracts in these important viral antigens	1786:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	10	48	theme	viral	1828:1832	arg1	antigens					1834:1841	these important viral antigens	1812:1841	these important viral antigens	1812:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	0	49	dep	Deciphering	0:10	arg1	Enrichment					77:86	Enrichment	77:86	Enrichment	77:86	Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment.					
29936827	0	49	dep	Deciphering	0:10	arg1	Cleavage					64:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Solid-Phase Chemoenzymatic Cleavage	37:71	Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment.					
29936827	9	50	theme	known	1472:1476	arg1	O-glycosites					1478:1489	known O-glycosites	1472:1489	known O-glycosites with high accuracy and confidence	1472:1523	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	9	51	from	method	1427:1432	arg1	glycoproteins					1446:1458	standard glycoproteins	1437:1458	standard glycoproteins	1437:1458	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	2	52	theme	wide	321:324	arg1	range					326:330	a wide range	319:330	a wide range of biological functions such as barrier formation against intestinal microbes	319:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	52	theme	wide	321:324	arg1	formation					372:380	barrier formation	364:380	barrier formation against intestinal microbes	364:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	5	53	dep	core	849:852	arg1	disaccharides					876:888	O-linked disaccharides	867:888	O-linked disaccharides	867:888	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	10	54	from	tracts	1802:1807	arg1	antigens					1834:1841	these important viral antigens	1812:1841	these important viral antigens	1812:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	2	55	theme	functions	346:354	arg1	range					326:330	a wide range	319:330	a wide range of biological functions such as barrier formation against intestinal microbes	319:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	55	theme	functions	346:354	arg1	formation					372:380	barrier formation	364:380	barrier formation against intestinal microbes	364:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	10	56	theme	Zika	1683:1686	arg1	virus					1688:1692	Zika virus	1683:1692	Zika virus recombinant glycoproteins	1683:1718	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	3	57	theme	specific	582:589	arg1	enzymes					591:597	specific enzymes	582:597	specific enzymes available for their release	582:625	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	11	58	from	investigation	1890:1902	arg1	diseases					1934:1941	diseases	1934:1941	diseases	1934:1941	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	11	58	from	investigation	1890:1902	arg1	contexts					1964:1971	other biomedical contexts	1947:1971	other biomedical contexts	1947:1971	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	5	59	theme	core	860:863	arg1	removal					838:844	the removal	834:844	the removal of core 1 and core 3 O-linked disaccharides from glycoproteins	834:907	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	2	60	theme	biological	335:344	arg1	functions					346:354	biological functions	335:354	biological functions such as barrier formation against intestinal microbes	335:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	60	theme	biological	335:344	arg1	formation					372:380	barrier formation	364:380	barrier formation against intestinal microbes	364:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	11	61	theme	other	1947:1951	arg1	contexts					1964:1971	other biomedical contexts	1947:1971	other biomedical contexts	1947:1971	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	2	62	theme	cellular	240:247	arg1	proteins					249:256	mammalian cellular proteins	230:256	mammalian cellular proteins	230:256	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	4	63	from	analysis	649:656	arg1	glycosylation					670:682	O-linked glycosylation	661:682	O-linked glycosylation	661:682	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	5	64	theme	O-linked	867:874	arg1	disaccharides					876:888	O-linked disaccharides	867:888	O-linked disaccharides	867:888	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	4	65	theme	O-linked	661:668	arg1	glycosylation					670:682	O-linked glycosylation	661:682	O-linked glycosylation	661:682	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	10	66	theme	recombinant	1694:1704	arg1	glycoproteins					1706:1718	Zika virus recombinant glycoproteins	1683:1718	Zika virus recombinant glycoproteins	1683:1718	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	3	67	theme	available	599:607	arg1	enzymes					591:597	specific enzymes	582:597	specific enzymes available for their release	582:625	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	9	68	theme	8-fold	1543:1548	arg1	glycosites					1555:1564	up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity	1537:1630	up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity	1537:1630	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	10	69	theme	important	1818:1826	arg1	antigens					1834:1841	these important viral antigens	1812:1841	these important viral antigens	1812:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	4	70	theme	equivalent	723:732	arg1	enzymes					734:740	equivalent enzymes	723:740	equivalent enzymes	723:740	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	11	71	theme	protein	1907:1913	arg1	O-glycosylation					1915:1929	protein O-glycosylation	1907:1929	protein O-glycosylation	1907:1929	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	10	72	gly	O-glycosites	1746:1757	arg2	O-glycosites					1746:1757	their dominant O-glycosites	1731:1757	their dominant O-glycosites	1731:1757	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	10	73	from	antigens	1834:1841	arg1	set					1779:1781	a basis set	1771:1781	a basis set of O-glycosylation tracts in these important viral antigens	1771:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	10	73	from	antigens	1834:1841	arg1	tracts					1802:1807	O-glycosylation tracts	1786:1807	O-glycosylation tracts in these important viral antigens	1786:1841	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	4	74	link	O-linked	661:668	arg1	glycosylation					670:682	O-linked glycosylation	661:682	O-linked glycosylation	661:682	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	5	75	gly	glycopeptides	996:1008	arg2	glycopeptides					996:1008	glycopeptides	996:1008	glycopeptides	996:1008	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	2	76	from	selectins	448:456	arg1	system					493:498	the innate immune system	475:498	the innate immune system	475:498	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	7	77	theme	heterogeneous	1254:1266	arg1	O-glycosylation					1268:1282	heterogeneous O-glycosylation	1254:1282	heterogeneous O-glycosylation	1254:1282	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	5	78	link	O-linked	867:874	arg1	disaccharides					876:888	O-linked disaccharides	867:888	O-linked disaccharides	867:888	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	5	79	from	glycoproteins	895:907	arg1	removal					838:844	the removal	834:844	the removal of core 1 and core 3 O-linked disaccharides from glycoproteins	834:907	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	5	80	theme	types	928:932	arg1	analysis					910:917	analysis	910:917	analysis of other types of O-glycans	910:945	Although O-glycosidase can catalyze the removal of core 1 and core 3 O-linked disaccharides from glycoproteins, analysis of other types of O-glycans remains difficult, particularly when residing on glycopeptides.					
29936827	6	81	theme	novel	1031:1035	arg1	approach					1052:1059	a novel chemoenzymatic approach	1029:1059	a novel chemoenzymatic approach driven by a newly available O-protease	1029:1098	Here, we describe a novel chemoenzymatic approach driven by a newly available O-protease and solid phase platform.					
29936827	2	82	from	galectins	462:470	arg1	system					493:498	the innate immune system	475:498	the innate immune system	475:498	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	10	83	gly	glycoproteins	1706:1718	arg1	glycoproteins					1706:1718	Zika virus recombinant glycoproteins	1683:1718	Zika virus recombinant glycoproteins	1683:1718	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	9	84	theme	increased	1608:1616	arg1	heterogeneity					1618:1630	concomitant increased heterogeneity	1596:1630	concomitant increased heterogeneity	1596:1630	We establish the method on standard glycoproteins, confirming known O-glycosites with high accuracy and confidence, and reveal up to 8-fold more glycosites than previously reported with concomitant increased heterogeneity.					
29936827	8	85	theme	solid-phase	1388:1398	arg1	platform					1400:1407	a solid-phase platform	1386:1407	a solid-phase platform	1386:1407	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	4	86	theme	O-glycans	787:795	arg1	lack					715:718	the lack	711:718	the lack of equivalent enzymes	711:740	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	4	86	theme	O-glycans	787:795	arg1	heterogeneity					770:782	the inherent structural heterogeneity	746:782	the inherent structural heterogeneity of O-glycans	746:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	4	87	gly	heterogeneity	770:782	arg1	enzymes					734:740	equivalent enzymes	723:740	equivalent enzymes	723:740	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	4	87	gly	heterogeneity	770:782	arg1	O-glycans					787:795	O-glycans	787:795	O-glycans	787:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	10	88	theme	dominant	1737:1744	arg1	O-glycosites					1746:1757	their dominant O-glycosites	1731:1757	their dominant O-glycosites	1731:1757	This technique is further applied for analysis of Zika virus recombinant glycoproteins, revealing their dominant O-glycosites and setting a basis set of O-glycosylation tracts in these important viral antigens.					
29936827	0	89	theme	Protein	12:18	arg1	O-Glycosylation					20:34	Protein O-Glycosylation	12:34	Protein O-Glycosylation	12:34	Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment.					
29936827	12	90	theme	indispensable	2003:2015	arg1	tool					2017:2020	an indispensable tool	2000:2020	an indispensable tool for investigations	2000:2039	This method should become an indispensable tool for investigations where O-glycosylation is central.					
29936827	6	91	theme	solid	1104:1108	arg1	platform					1116:1123	solid phase platform	1104:1123	solid phase platform	1104:1123	Here, we describe a novel chemoenzymatic approach driven by a newly available O-protease and solid phase platform.					
29936827	4	92	theme	inherent	750:757	arg1	heterogeneity					770:782	the inherent structural heterogeneity	746:782	the inherent structural heterogeneity of O-glycans	746:795	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	2	93	theme	signaling	424:432	arg1	modification					291:302	this modification	286:302	this modification	286:302	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	2	93	theme	signaling	424:432	arg1	molecules					434:442	signaling molecules	424:442	signaling molecules for selectins and galectins in the innate immune system	424:498	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	7	94	theme	N-glycan	1189:1196	arg1	profile					1198:1204	N-glycan profile	1189:1204	N-glycan profile	1189:1204	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	2	95	theme	innate	479:484	arg1	system					493:498	the innate immune system	475:498	the innate immune system	475:498	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	7	96	theme	peptides	1179:1186	arg1	mapping					1243:1249	mapping	1243:1249	mapping of heterogeneous O-glycosylation	1243:1282	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	96	theme	peptides	1179:1186	arg1	assignment					1150:1159	the assignment	1146:1159	the assignment of O-glycosylated peptides	1146:1186	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	96	theme	peptides	1179:1186	arg1	profile					1198:1204	N-glycan profile	1189:1204	N-glycan profile	1189:1204	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	7	96	theme	peptides	1179:1186	arg1	linkage					1230:1236	sialyl O-glycopeptides linkage	1207:1236	sialyl O-glycopeptides linkage	1207:1236	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	11	97	theme	biomedical	1953:1962	arg1	contexts					1964:1971	other biomedical contexts	1947:1971	other biomedical contexts	1947:1971	Our approach can serve as a benchmark for the investigation of protein O-glycosylation in diseases and other biomedical contexts.					
29936827	3	98	theme	glycosylation	510:522	arg1	analysis					524:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis	501:531	N-linked glycosylation analysis has been greatly facilitated owing to a range of specific enzymes available for their release.					
29936827	8	99	dep	O-glycopeptides	1327:1341	arg1	enriched					1371:1378	enriched	1371:1378	enriched using a solid-phase platform	1371:1407	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	8	99	dep	O-glycopeptides	1327:1341	arg1	generated					1343:1351	generated	1343:1351	generated by O-protease	1343:1365	For the first time, we can analyze intact O-glycopeptides generated by O-protease and enriched using a solid-phase platform.					
29936827	7	100	gly	O-glycopeptides	1214:1228	arg2	O-glycopeptides					1214:1228	sialyl O-glycopeptides linkage	1207:1236	sialyl O-glycopeptides linkage	1207:1236	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
29936827	2	101	theme	barrier	364:370	arg1	formation					372:380	barrier formation	364:380	barrier formation against intestinal microbes	364:408	Over 50% of mammalian cellular proteins are typically glycosylated; this modification is involved in a wide range of biological functions such as barrier formation against intestinal microbes and serves as signaling molecules for selectins and galectins in the innate immune system.					
29936827	4	102	theme	system-wide	637:647	arg1	analysis					649:656	system-wide analysis	637:656	system-wide analysis on O-linked glycosylation	637:682	However, system-wide analysis on O-linked glycosylation remains a challenge due to the lack of equivalent enzymes and the inherent structural heterogeneity of O-glycans.					
29936827	7	103	theme	O-glycopeptides	1214:1228	arg1	linkage					1230:1236	sialyl O-glycopeptides linkage	1207:1236	sialyl O-glycopeptides linkage	1207:1236	This method enables the assignment of O-glycosylated peptides, N-glycan profile, sialyl O-glycopeptides linkage, and mapping of heterogeneous O-glycosylation.					
30659587	7	0	from	sites	1413:1417	arg1	levels					1368:1373	high levels	1363:1373	high levels of galactosylation at the fusion body sites	1363:1417	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	9	1	theme	body	1643:1646	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of the fusion body	1605:1646	All N-glycan variants exhibited weak effects on the biological activity of the fusion body.					
30659587	5	2	theme	sialic	973:978	arg1	acid					980:983	sialic acid	973:983	sialic acid	973:983	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	3	gly	glycosylation	914:926	arg2	two					898:900	two	898:900	two	898:900	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	3	gly	glycosylation	914:926	arg2	sites					928:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	10	4	theme	enzymatic	1680:1688	arg1	glycoengineering					1690:1705	enzymatic glycoengineering	1680:1705	enzymatic glycoengineering	1680:1705	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	10	4	theme	enzymatic	1680:1688	arg1	media					1654:1658	Both media supplementation and enzymatic glycoengineering	1649:1705	Both media supplementation and enzymatic glycoengineering	1649:1705	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	1	5	theme	proteins	183:190	arg1	safety					161:166	safety	161:166	safety	161:166	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	1	5	theme	proteins	183:190	arg1	efficacy					148:155	efficacy	148:155	efficacy	148:155	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	2	6	theme	hamster	209:215	arg1	ovary					217:221	Chinese hamster ovary	201:221	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	6	7	theme	glycosylation	1182:1194	arg1	heterogeneity					1196:1208	the Fc-site glycosylation heterogeneity	1170:1208	the Fc-site glycosylation heterogeneity	1170:1208	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	5	8	theme	supplement	1108:1117	arg1	presence					1088:1095	the presence	1084:1095	the presence of a media supplement	1084:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	2	9	theme	antibody	302:309	arg1	glycoprofiles					273:285	consistent glycoprofiles	262:285	consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites	262:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	5	10	theme	fusion	902:907	arg1	sites					928:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	3	11	gly	sialylated	627:636	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	2	12	theme	fed-batch	223:231	arg1	batches					246:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	5	13	theme	glycosylation	914:926	arg1	sites					928:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	8	14	theme	FcγRIIIa	1521:1528	arg1	affinity					1547:1554	increased FcγRIIIa receptor binding affinity	1511:1554	increased FcγRIIIa receptor binding affinity	1511:1554	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	6	15	theme	classical	1276:1284	arg1	antibodies					1297:1306	classical monoclonal antibodies	1276:1306	classical monoclonal antibodies	1276:1306	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	4	16	theme	FcγRIIIa	774:781	arg1	affinity					800:807	the FcγRIIIa receptor binding affinity	770:807	the FcγRIIIa receptor binding affinity	770:807	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	3	17	theme	sialylated	627:636	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	5	18	theme	acid	980:983	arg1	degree					963:968	a high degree	956:968	a high degree of sialic acid	956:983	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	18	theme	acid	980:983	arg1	behavior					1072:1079	a site-specific behavior	1056:1079	a site-specific behavior in the presence of a media supplement	1056:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	18	theme	acid	980:983	arg1	degree					1029:1034	a higher degree	1020:1034	a higher degree of antennarity	1020:1049	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	18	theme	acid	980:983	arg1	structures					1008:1017	more complex N-glycan structures	986:1017	more complex N-glycan structures	986:1017	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	8	19	theme	binding	1539:1545	arg1	affinity					1547:1554	increased FcγRIIIa receptor binding affinity	1511:1554	increased FcγRIIIa receptor binding affinity	1511:1554	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	4	20	theme	binding	792:798	arg1	affinity					800:807	the FcγRIIIa receptor binding affinity	770:807	the FcγRIIIa receptor binding affinity	770:807	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	3	21	theme	agalactosylated	577:591	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	9	22	theme	weak	1589:1592	arg1	effects					1594:1600	weak effects	1589:1600	weak effects	1589:1600	All N-glycan variants exhibited weak effects on the biological activity of the fusion body.					
30659587	4	23	theme	glycosylation	744:756	arg1	variants					758:765	the glycosylation variants	740:765	the glycosylation variants	740:765	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	8	24	with	Variants	1420:1427	arg1	fucosylation					1443:1454	low core fucosylation	1434:1454	low core fucosylation	1434:1454	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	0	25	theme	bioactivity	59:69	arg1	testing					71:77	in vitro bioactivity testing	50:77	in vitro bioactivity testing	50:77	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	2	26	theme	culture	238:244	arg1	batches					246:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	5	27	theme	high	958:961	arg1	degree					963:968	a high degree	956:968	a high degree of sialic acid	956:983	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	28	from	degree	963:968	arg1	presence					1088:1095	the presence	1084:1095	the presence of a media supplement	1084:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	4	29	dep	activity	828:835	arg1	resonance					869:877	surface plasmon resonance	853:877	surface plasmon resonance	853:877	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	4	29	dep	activity	828:835	arg1	cell-based					838:847	cell-based	838:847	cell-based	838:847	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	7	30	theme	Enzymatic	1309:1317	arg1	glycoengineering					1319:1334	Enzymatic glycoengineering	1309:1334	Enzymatic glycoengineering	1309:1334	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	5	31	theme	higher	1022:1027	arg1	degree					1029:1034	a higher degree	1020:1034	a higher degree of antennarity	1020:1049	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	8	32	theme	low	1434:1436	arg1	fucosylation					1443:1454	low core fucosylation	1434:1454	low core fucosylation	1434:1454	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	9	33	theme	fusion	1636:1641	arg1	body					1643:1646	the fusion body	1632:1646	the fusion body	1632:1646	All N-glycan variants exhibited weak effects on the biological activity of the fusion body.					
30659587	1	34	dep	efficacy	148:155	arg1	the					144:146	the	144:146	the	144:146	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	5	35	theme	N-glycan	999:1006	arg1	structures					1008:1017	more complex N-glycan structures	986:1017	more complex N-glycan structures	986:1017	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	7	36	theme	fusion	1401:1406	arg1	sites					1413:1417	the fusion body sites	1397:1417	the fusion body sites	1397:1417	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	3	37	theme	specific	392:399	arg1	concentrations					401:414	specific concentrations	392:414	specific concentrations in cell culture	392:430	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	4	38	theme	biological	817:826	arg1	activity					828:835	the biological activity	813:835	the biological activity (cell-based and surface plasmon resonance)	813:878	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	10	39	theme	glycostructures	1777:1791	arg1	effect					1767:1772	the effect	1763:1772	the effect of glycostructures on the biological activity	1763:1818	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	0	40	theme	site-distinct	14:26	arg1	variants					37:44	site-distinct N-glycan variants	14:44	site-distinct N-glycan variants	14:44	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	1	41	theme	product	106:112	arg1	Glycosylation					80:92	Glycosylation	80:92	Glycosylation	80:92	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	1	41	theme	product	106:112	arg1	attribute					122:130	a critical product quality attribute	95:130	a critical product quality attribute	95:130	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	3	42	theme	N-glycan	483:490	arg1	population					500:509	a diverse N-glycan variant population	473:509	a diverse N-glycan variant population	473:509	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	0	43	theme	variants	37:44	arg1	Generation					0:9	Generation	0:9	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.	0:78	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	0	44	dep	in	50:51	arg1	vitro					53:57	vitro	53:57	vitro	53:57	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	0	45	theme	in	50:51	arg1	testing					71:77	in vitro bioactivity testing	50:77	in vitro bioactivity testing	50:77	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	7	46	theme	high	1363:1366	arg1	levels					1368:1373	high levels	1363:1373	high levels of galactosylation at the fusion body sites	1363:1417	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	2	47	gly	N-glycosylation	338:352	arg2	three					322:326	three	322:326	three	322:326	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	2	47	gly	N-glycosylation	338:352	arg2	sites					354:358	three different N-glycosylation sites	322:358	three different N-glycosylation sites	322:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	4	48	theme	plasmon	861:867	arg1	resonance					869:877	surface plasmon resonance	853:877	surface plasmon resonance	853:877	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	10	49	from	effect	1767:1772	arg1	activity					1811:1818	the biological activity	1796:1818	the biological activity	1796:1818	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	6	50	theme	media	1143:1147	arg1	supplements					1149:1159	the media supplements	1139:1159	the media supplements	1139:1159	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	4	51	theme	Site-specific	645:657	arg1	profiles					673:680	Site-specific glycosylation profiles	645:680	Site-specific glycosylation profiles	645:680	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	3	52	theme	afucosylated	550:561	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	3	53	theme	mannose	541:547	arg1	forms					638:642	high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms	536:642	high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms	536:642	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	6	54	theme	other	1127:1131	arg1	hand					1133:1136	the other hand	1123:1136	the other hand	1123:1136	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	9	55	theme	N-glycan	1561:1568	arg1	variants					1570:1577	All N-glycan variants	1557:1577	All N-glycan variants	1557:1577	All N-glycan variants exhibited weak effects on the biological activity of the fusion body.					
30659587	2	56	theme	Fc-fusion	292:300	arg1	antibody					302:309	a Fc-fusion antibody	290:309	a Fc-fusion antibody comprizing three different N-glycosylation sites	290:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	3	57	theme	asialylated	610:620	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	6	58	theme	various	1227:1233	arg1	studies					1235:1241	the various studies	1223:1241	the various studies described in the literature with classical monoclonal antibodies	1223:1306	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	3	59	theme	fucosylated	564:574	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	4	60	from	effect	730:735	arg1	affinity					800:807	the FcγRIIIa receptor binding affinity	770:807	the FcγRIIIa receptor binding affinity	770:807	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	4	60	from	effect	730:735	arg1	activity					828:835	the biological activity	813:835	the biological activity (cell-based and surface plasmon resonance)	813:878	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	4	61	gly	glycopeptide	701:712	arg2	glycopeptide					701:712	glycopeptide mapping	701:720	glycopeptide mapping	701:720	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	2	62	theme	different	328:336	arg1	sites					354:358	three different N-glycosylation sites	322:358	three different N-glycosylation sites	322:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	1	63	theme	therapeutic	171:181	arg1	proteins					183:190	therapeutic proteins	171:190	therapeutic proteins	171:190	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	3	64	theme	galactosylated	594:607	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	8	65	theme	increased	1511:1519	arg1	affinity					1547:1554	increased FcγRIIIa receptor binding affinity	1511:1554	increased FcγRIIIa receptor binding affinity	1511:1554	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	4	66	theme	variants	758:765	arg1	effect					730:735	the effect	726:735	the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance)	726:878	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	9	67	theme	biological	1609:1618	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of the fusion body	1605:1646	All N-glycan variants exhibited weak effects on the biological activity of the fusion body.					
30659587	10	68	theme	biological	1800:1809	arg1	activity					1811:1818	the biological activity	1796:1818	the biological activity	1796:1818	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	10	69	dep	media	1654:1658	arg1	supplementation					1660:1674	supplementation	1660:1674	supplementation	1660:1674	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	10	69	dep	media	1654:1658	arg1	glycoengineering					1690:1705	enzymatic glycoengineering	1680:1705	enzymatic glycoengineering	1680:1705	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	10	69	dep	media	1654:1658	arg1	media					1654:1658	Both media supplementation and enzymatic glycoengineering	1649:1705	Both media supplementation and enzymatic glycoengineering	1649:1705	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	2	70	theme	cell	233:236	arg1	batches					246:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	5	71	from	behavior	1072:1079	arg1	presence					1088:1095	the presence	1084:1095	the presence of a media supplement	1084:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	72	theme	body	909:912	arg1	sites					928:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites	894:932	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	8	73	theme	receptor	1530:1537	arg1	affinity					1547:1554	increased FcγRIIIa receptor binding affinity	1511:1554	increased FcγRIIIa receptor binding affinity	1511:1554	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	6	74	theme	monoclonal	1286:1295	arg1	antibodies					1297:1306	classical monoclonal antibodies	1276:1306	classical monoclonal antibodies	1276:1306	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	4	75	theme	glycopeptide	701:712	arg1	mapping					714:720	glycopeptide mapping	701:720	glycopeptide mapping	701:720	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	2	76	theme	ovary	217:221	arg1	batches					246:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	1	77	theme	quality	114:120	arg1	Glycosylation					80:92	Glycosylation	80:92	Glycosylation	80:92	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	1	77	theme	quality	114:120	arg1	attribute					122:130	a critical product quality attribute	95:130	a critical product quality attribute	95:130	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	3	78	gly	asialylated	610:620	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	8	79	theme	high	1480:1483	arg1	glycans					1493:1499	high mannose glycans	1480:1499	high mannose glycans	1480:1499	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	2	80	theme	consistent	262:271	arg1	glycoprofiles					273:285	consistent glycoprofiles	262:285	consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites	262:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	4	81	theme	receptor	783:790	arg1	affinity					800:807	the FcγRIIIa receptor binding affinity	770:807	the FcγRIIIa receptor binding affinity	770:807	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	5	82	theme	media	1102:1106	arg1	supplement					1108:1117	a media supplement	1100:1117	a media supplement	1100:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	3	83	gly	fucosylated	564:574	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	6	84	theme	Fc-site	1174:1180	arg1	heterogeneity					1196:1208	the Fc-site glycosylation heterogeneity	1170:1208	the Fc-site glycosylation heterogeneity	1170:1208	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	6	85	with	literature	1260:1269	arg1	antibodies					1297:1306	classical monoclonal antibodies	1276:1306	classical monoclonal antibodies	1276:1306	On the other hand, the media supplements affected the Fc-site glycosylation heterogeneity similarly to the various studies described in the literature with classical monoclonal antibodies.					
30659587	3	86	gly	afucosylated	550:561	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	3	87	from	concentrations	401:414	arg1	culture					424:430	cell culture	419:430	cell culture	419:430	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	7	88	theme	body	1408:1411	arg1	sites					1413:1417	the fusion body sites	1397:1417	the fusion body sites	1397:1417	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	3	89	theme	media	371:375	arg1	supplements					377:387	media supplements	371:387	media supplements	371:387	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	3	90	theme	diverse	475:481	arg1	population					500:509	a diverse N-glycan variant population	473:509	a diverse N-glycan variant population	473:509	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	5	91	theme	complex	991:997	arg1	structures					1008:1017	more complex N-glycan structures	986:1017	more complex N-glycan structures	986:1017	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	3	92	theme	cell	419:422	arg1	culture					424:430	cell culture	419:430	cell culture	419:430	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	8	93	theme	core	1438:1441	arg1	fucosylation					1443:1454	low core fucosylation	1434:1454	low core fucosylation	1434:1454	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	10	94	theme	sufficient	1732:1741	arg1	diversity					1743:1751	sufficient diversity	1732:1751	sufficient diversity	1732:1751	Both media supplementation and enzymatic glycoengineering are suitable to generate sufficient diversity to assess the effect of glycostructures on the biological activity.					
30659587	0	95	theme	N-glycan	28:35	arg1	variants					37:44	site-distinct N-glycan variants	14:44	site-distinct N-glycan variants	14:44	Generation of site-distinct N-glycan variants for in vitro bioactivity testing.					
30659587	1	96	theme	critical	97:104	arg1	Glycosylation					80:92	Glycosylation	80:92	Glycosylation	80:92	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	1	96	theme	critical	97:104	arg1	attribute					122:130	a critical product quality attribute	95:130	a critical product quality attribute	95:130	Glycosylation, a critical product quality attribute, may affect the efficacy and safety of therapeutic proteins in vivo.					
30659587	7	97	theme	galactosylation	1378:1392	arg1	levels					1368:1373	high levels	1363:1373	high levels of galactosylation at the fusion body sites	1363:1417	Enzymatic glycoengineering solely managed to generate high levels of galactosylation at the fusion body sites.					
30659587	5	98	theme	site-specific	1058:1070	arg1	behavior					1072:1079	a site-specific behavior	1056:1079	a site-specific behavior in the presence of a media supplement	1056:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	3	99	theme	enzymatic	445:453	arg1	glycoengineering					455:470	enzymatic glycoengineering	445:470	enzymatic glycoengineering	445:470	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	3	100	theme	variant	492:498	arg1	population					500:509	a diverse N-glycan variant population	473:509	a diverse N-glycan variant population	473:509	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	8	101	gly	fucosylation	1443:1454	arg1	Variants					1420:1427	Variants	1420:1427	Variants with low core fucosylation, and to a lower extent, high mannose glycans	1420:1499	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	2	102	theme	Chinese	201:207	arg1	ovary					217:221	Chinese hamster ovary	201:221	Chinese hamster ovary fed-batch cell culture batches	201:252	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	8	103	theme	lower	1466:1470	arg1	extent					1472:1477	a lower extent	1464:1477	a lower extent	1464:1477	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	3	104	theme	high	536:539	arg1	mannose					541:547	high mannose	536:547	high mannose	536:547	By adding media supplements at specific concentrations in cell culture and applying enzymatic glycoengineering, a diverse N-glycan variant population was generated, including high mannose, afucosylated, fucosylated, agalactosylated, galactosylated, asialylated, and sialylated forms.					
30659587	5	105	from	structures	1008:1017	arg1	presence					1088:1095	the presence	1084:1095	the presence of a media supplement	1084:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	106	theme	antennarity	1039:1049	arg1	degree					963:968	a high degree	956:968	a high degree of sialic acid	956:983	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	106	theme	antennarity	1039:1049	arg1	behavior					1072:1079	a site-specific behavior	1056:1079	a site-specific behavior in the presence of a media supplement	1056:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	106	theme	antennarity	1039:1049	arg1	degree					1029:1034	a higher degree	1020:1034	a higher degree of antennarity	1020:1049	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	5	106	theme	antennarity	1039:1049	arg1	structures					1008:1017	more complex N-glycan structures	986:1017	more complex N-glycan structures	986:1017	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
30659587	4	107	theme	glycosylation	659:671	arg1	profiles					673:680	Site-specific glycosylation profiles	645:680	Site-specific glycosylation profiles	645:680	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	4	108	theme	surface	853:859	arg1	resonance					869:877	surface plasmon resonance	853:877	surface plasmon resonance	853:877	Site-specific glycosylation profiles were elucidated by glycopeptide mapping and the effect of the glycosylation variants on the FcγRIIIa receptor binding affinity and the biological activity (cell-based and surface plasmon resonance) was assessed.					
30659587	2	109	theme	N-glycosylation	338:352	arg1	sites					354:358	three different N-glycosylation sites	322:358	three different N-glycosylation sites	322:358	Chinese hamster ovary fed-batch cell culture batches yielded consistent glycoprofiles of a Fc-fusion antibody comprizing three different N-glycosylation sites.					
30659587	8	110	theme	mannose	1485:1491	arg1	glycans					1493:1499	high mannose glycans	1480:1499	high mannose glycans	1480:1499	Variants with low core fucosylation, and to a lower extent, high mannose glycans exhibited increased FcγRIIIa receptor binding affinity.					
30659587	5	111	from	degree	1029:1034	arg1	presence					1088:1095	the presence	1084:1095	the presence of a media supplement	1084:1117	The two fusion body glycosylation sites were characterized by a high degree of sialic acid, more complex N-glycan structures, a higher degree of antennarity, and a site-specific behavior in the presence of a media supplement.					
31411531	7	0	theme	alpha	1258:1262	arg1	acid					1282:1285	only alpha 2,3-linked sialic acid	1253:1285	only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1253:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	1	theme	sialylated	1132:1141	arg1	glycans					1143:1149	sialylated glycans	1132:1149	sialylated glycans	1132:1149	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	2	theme	glycan-CH2	1441:1450	arg1	interaction					1459:1469	the glycan-CH2 domain interaction	1437:1469	the glycan-CH2 domain interaction	1437:1469	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	3	from	acid	1282:1285	arg1	6-arm					1294:1298	the 6-arm	1290:1298	the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1290:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	2	4	theme	serum	399:403	arg1	Fc					409:410	human serum IgG Fc	393:410	human serum IgG Fc	393:410	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	4	5	theme	linkages	669:676	arg1	impact					637:642	The impact	633:642	The impact of different sialylation linkages to the structure of IgG	633:700	The impact of different sialylation linkages to the structure of IgG has not been determined.					
31411531	6	6	from	domain	1049:1054	arg1	IgG					1100:1102	human-derived IgG	1086:1102	human-derived IgG	1086:1102	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	6	6	from	domain	1049:1054	arg1	IgG					1073:1075	CHO-expressed IgG	1059:1075	CHO-expressed IgG	1059:1075	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	3	7	theme	sialic	532:537	arg1	acids					539:543	sialic acids	532:543	sialic acids	532:543	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	8	8	link	2,3-linked	1584:1593	arg1	acid					1602:1605	the alpha 2,3-linked sialic acid	1574:1605	the alpha 2,3-linked sialic acid on the 3-arm	1574:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	2	9	theme	alpha	334:338	arg1	2,3-linkage					340:350	an alpha 2,3-linkage	331:350	an alpha 2,3-linkage	331:350	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	1	10	theme	antibody-dependent	170:187	arg1	cytotoxicity					203:214	antibody-dependent cell-mediated cytotoxicity	170:214	antibody-dependent cell-mediated cytotoxicity	170:214	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	2	11	from	acids	384:388	arg1	Fc					409:410	human serum IgG Fc	393:410	human serum IgG Fc	393:410	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	7	12	gly	sialylated	1132:1141	arg1	glycans					1143:1149	sialylated glycans	1132:1149	sialylated glycans	1132:1149	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	2	13	gly	sialylation	276:286	arg1	N-glycans					299:307	complex N-glycans	291:307	complex N-glycans	291:307	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	6	14	theme	human-derived	1086:1098	arg1	IgG					1100:1102	human-derived IgG	1086:1102	human-derived IgG	1086:1102	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	4	15	theme	different	647:655	arg1	linkages					669:676	different sialylation linkages	647:676	different sialylation linkages	647:676	The impact of different sialylation linkages to the structure of IgG has not been determined.					
31411531	8	16	theme	2,6-linked	1482:1491	arg1	acid					1500:1503	The alpha 2,6-linked sialic acid	1472:1503	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG)	1472:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	2	17	theme	complex	291:297	arg1	N-glycans					299:307	complex N-glycans	291:307	complex N-glycans	291:307	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	7	18	theme	CH2	1370:1372	arg1	domain					1374:1379	the CH2 domain	1366:1379	the CH2 domain	1366:1379	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	19	contain	have	1628:1631	arg2	effect					1652:1657	this destabilizing effect	1633:1657	this destabilizing effect	1633:1657	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	19	contain	have	1628:1631	arg1	acid					1500:1503	The alpha 2,6-linked sialic acid	1472:1503	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG)	1472:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	19	contain	have	1628:1631	arg1	acid					1602:1605	the alpha 2,3-linked sialic acid	1574:1605	the alpha 2,3-linked sialic acid on the 3-arm	1574:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	20	theme	sialylated	1311:1320	arg1	glycans					1322:1328	the major sialylated glycans	1301:1328	the major sialylated glycans in CHO-expressed IgG1	1301:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	21	theme	only	1253:1256	arg1	acid					1282:1285	only alpha 2,3-linked sialic acid	1253:1285	only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1253:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	3	22	theme	2,6-sialyltransferase	605:625	arg1	gene					627:630	the alpha 2,6-sialyltransferase gene	595:630	the alpha 2,6-sialyltransferase gene	595:630	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	8	23	gly	sialylated	1529:1538	arg1	glycan					1540:1545	the major sialylated glycan	1519:1545	the major sialylated glycan in human-derived IgG	1519:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	24	gly	sialylated	1311:1320	arg1	glycans					1322:1328	the major sialylated glycans	1301:1328	the major sialylated glycans in CHO-expressed IgG1	1301:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	0	25	from	Impact	0:5	arg1	structure					41:49	IgG structure	37:49	IgG structure	37:49	Impact of Fc N-glycan sialylation on IgG structure.					
31411531	8	26	from	acid	1500:1503	arg1	3-arm					1512:1516	the 3-arm	1508:1516	the 3-arm (the major sialylated glycan in human-derived IgG)	1508:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	26	from	acid	1500:1503	arg1	3-arm					1614:1618	the 3-arm	1610:1618	the 3-arm	1610:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	27	link	2,6-linked	1482:1491	arg1	acid					1500:1503	The alpha 2,6-linked sialic acid	1472:1503	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG)	1472:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	1	28	theme	Human	52:56	arg1	antibodies					62:71	Human IgG antibodies	52:71	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans	52:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	6	29	theme	sialic	984:989	arg1	glycans					1007:1013	sialic acid-containing glycans	984:1013	sialic acid-containing glycans	984:1013	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	8	30	theme	major	1523:1527	arg1	glycan					1540:1545	the major sialylated glycan	1519:1545	the major sialylated glycan in human-derived IgG	1519:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	3	31	theme	Recombinant	453:463	arg1	IgGs					465:468	Recombinant IgGs	453:468	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells	453:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	7	32	theme	proteolysis	1215:1225	arg1	experiment					1227:1236	the limited proteolysis experiment	1203:1236	the limited proteolysis experiment	1203:1236	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	6	33	link	human-derived	925:937	arg1	IgG1					957:960	human-derived and CHO-expressed IgG1	925:960	human-derived and CHO-expressed IgG1	925:960	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	1	34	theme	terminal	84:91	arg1	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	2	35	from	sialylation	276:286	arg1	N-glycans					299:307	complex N-glycans	291:307	complex N-glycans	291:307	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	7	36	theme	CHO-expressed	1333:1345	arg1	IgG1					1347:1350	CHO-expressed IgG1	1333:1350	CHO-expressed IgG1	1333:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	37	theme	human-derived	1550:1562	arg1	IgG					1564:1566	human-derived IgG	1550:1566	human-derived IgG	1550:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	3	38	theme	Chinese	483:489	arg1	cells					511:515	Chinese hamster ovary (CHO) cells	483:515	Chinese hamster ovary (CHO) cells	483:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	5	39	theme	conformational	809:822	arg1	stability					824:832	the conformational stability	805:832	the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments	805:917	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	1	40	theme	2,6-linked	99:108	arg1	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	0	41	theme	sialylation	22:32	arg1	Impact					0:5	Impact	0:5	Impact of Fc N-glycan sialylation on IgG structure.	0:50	Impact of Fc N-glycan sialylation on IgG structure.					
31411531	5	42	theme	different	771:779	arg1	types					781:785	different types	771:785	different types of sialylation	771:800	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	6	43	theme	CH2	1045:1047	arg1	domain					1049:1054	the CH2 domain	1041:1054	the CH2 domain in CHO-expressed IgG, but not human-derived IgG	1041:1102	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	3	44	contain	have	527:530	arg1	IgGs					465:468	Recombinant IgGs	453:468	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells	453:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	3	44	contain	have	527:530	arg2	acids					539:543	sialic acids	532:543	sialic acids	532:543	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	3	45	theme	CHO	506:508	arg1	cells					511:515	Chinese hamster ovary (CHO) cells	483:515	Chinese hamster ovary (CHO) cells	483:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	0	46	theme	IgG	37:39	arg1	structure					41:49	IgG structure	37:49	IgG structure	37:49	Impact of Fc N-glycan sialylation on IgG structure.					
31411531	2	47	theme	terminal	267:274	arg1	sialylation					276:286	terminal sialylation	267:286	terminal sialylation on complex N-glycans	267:307	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	6	48	link	human-derived	1086:1098	arg1	IgG					1100:1102	human-derived IgG	1086:1102	human-derived IgG	1086:1102	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	3	49	theme	ovary	499:503	arg1	cells					511:515	Chinese hamster ovary (CHO) cells	483:515	Chinese hamster ovary (CHO) cells	483:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	1	50	theme	anti-inflammatory	228:244	arg1	properties					246:255	anti-inflammatory properties	228:255	anti-inflammatory properties	228:255	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	8	51	theme	sialic	1595:1600	arg1	acid					1602:1605	the alpha 2,3-linked sialic acid	1574:1605	the alpha 2,3-linked sialic acid on the 3-arm	1574:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	52	theme	major	1305:1309	arg1	glycans					1322:1328	the major sialylated glycans	1301:1328	the major sialylated glycans in CHO-expressed IgG1	1301:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	53	theme	destabilizing	1638:1650	arg1	effect					1652:1657	this destabilizing effect	1633:1657	this destabilizing effect	1633:1657	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	5	54	theme	types	781:785	arg1	impact					761:766	the impact	757:766	the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments	757:917	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	6	55	theme	human-derived	925:937	arg1	IgG1					957:960	human-derived and CHO-expressed IgG1	925:960	human-derived and CHO-expressed IgG1	925:960	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	2	56	theme	sialic	377:382	arg1	acids					384:388	sialic acids	377:388	sialic acids on human serum IgG Fc	377:410	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	7	57	theme	2,3-linked	1264:1273	arg1	acid					1282:1285	only alpha 2,3-linked sialic acid	1253:1285	only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1253:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	58	theme	structural	1110:1119	arg1	isomers					1121:1127	structural isomers	1110:1127	structural isomers of sialylated glycans	1110:1149	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	59	dep	3-arm	1512:1516	arg1	glycan					1540:1545	the major sialylated glycan	1519:1545	the major sialylated glycan in human-derived IgG	1519:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	60	theme	domain	1452:1457	arg1	interaction					1459:1469	the glycan-CH2 domain interaction	1437:1469	the glycan-CH2 domain interaction	1437:1469	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	5	61	theme	hydrogen/deuterium	849:866	arg1	exchange					868:875	hydrogen/deuterium exchange	849:875	hydrogen/deuterium exchange (HDX)	849:881	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	5	61	theme	hydrogen/deuterium	849:866	arg1	HDX					878:880	HDX	878:880	HDX	878:880	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	8	62	link	human-derived	1550:1562	arg1	IgG					1564:1566	human-derived IgG	1550:1566	human-derived IgG	1550:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	4	63	theme	sialylation	657:667	arg1	linkages					669:676	different sialylation linkages	647:676	different sialylation linkages	647:676	The impact of different sialylation linkages to the structure of IgG has not been determined.					
31411531	3	64	theme	alpha	553:557	arg1	2,3-linkages					559:570	alpha 2,3-linkages	553:570	alpha 2,3-linkages	553:570	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	1	65	theme	cell-mediated	189:201	arg1	cytotoxicity					203:214	antibody-dependent cell-mediated cytotoxicity	170:214	antibody-dependent cell-mediated cytotoxicity	170:214	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	7	66	link	2,3-linked	1264:1273	arg1	acid					1282:1285	only alpha 2,3-linked sialic acid	1253:1285	only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1253:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	67	from	glycan	1540:1545	arg1	IgG					1564:1566	human-derived IgG	1550:1566	human-derived IgG	1550:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	68	theme	steric	1408:1413	arg1	effect					1415:1420	the steric effect	1404:1420	the steric effect that decreases the glycan-CH2 domain interaction	1404:1469	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	69	from	acid	1602:1605	arg1	3-arm					1512:1516	the 3-arm	1508:1516	the 3-arm (the major sialylated glycan in human-derived IgG)	1508:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	69	from	acid	1602:1605	arg1	3-arm					1614:1618	the 3-arm	1610:1618	the 3-arm	1610:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	5	70	theme	IgG	837:839	arg1	stability					824:832	the conformational stability	805:832	the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments	805:917	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	8	71	theme	alpha	1476:1480	arg1	acid					1500:1503	The alpha 2,6-linked sialic acid	1472:1503	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG)	1472:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	8	72	theme	alpha	1578:1582	arg1	acid					1602:1605	the alpha 2,3-linked sialic acid	1574:1605	the alpha 2,3-linked sialic acid on the 3-arm	1574:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	1	73	theme	sialic	110:115	arg1	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	6	74	theme	CHO-expressed	943:955	arg1	IgG1					957:960	human-derived and CHO-expressed IgG1	925:960	human-derived and CHO-expressed IgG1	925:960	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	8	75	theme	sialic	1493:1498	arg1	acid					1500:1503	The alpha 2,6-linked sialic acid	1472:1503	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG)	1472:1567	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	1	76	contain	containing	73:82	arg1	antibodies					62:71	Human IgG antibodies	52:71	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans	52:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	1	76	contain	containing	73:82	arg2	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	3	77	theme	gene	627:630	arg1	lack					587:590	the lack	583:590	the lack of the alpha 2,6-sialyltransferase gene	583:630	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	3	78	theme	alpha	599:603	arg1	gene					627:630	the alpha 2,6-sialyltransferase gene	595:630	the alpha 2,6-sialyltransferase gene	595:630	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	1	79	theme	Fc	131:132	arg1	N-glycans					134:142	their Fc N-glycans	125:142	their Fc N-glycans	125:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	5	80	theme	limited	887:893	arg1	proteolysis					895:905	limited proteolysis	887:905	limited proteolysis	887:905	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	0	81	theme	Fc	10:11	arg1	sialylation					22:32	Fc N-glycan sialylation	10:32	Fc N-glycan sialylation	10:32	Impact of Fc N-glycan sialylation on IgG structure.					
31411531	4	82	theme	IgG	698:700	arg1	structure					685:693	the structure	681:693	the structure of IgG	681:700	The impact of different sialylation linkages to the structure of IgG has not been determined.					
31411531	6	83	theme	acid-containing	991:1005	arg1	glycans					1007:1013	sialic acid-containing glycans	984:1013	sialic acid-containing glycans	984:1013	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	8	84	theme	sialylated	1529:1538	arg1	glycan					1540:1545	the major sialylated glycan	1519:1545	the major sialylated glycan in human-derived IgG	1519:1566	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	7	85	theme	limited	1207:1213	arg1	experiment					1227:1236	the limited proteolysis experiment	1203:1236	the limited proteolysis experiment	1203:1236	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	1	86	theme	IgG	58:60	arg1	antibodies					62:71	Human IgG antibodies	52:71	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans	52:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	2	87	theme	IgG	405:407	arg1	Fc					409:410	human serum IgG Fc	393:410	human serum IgG Fc	393:410	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	5	88	theme	sialylation	790:800	arg1	types					781:785	different types	771:785	different types of sialylation	771:800	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	2	89	theme	human	393:397	arg1	Fc					409:410	human serum IgG Fc	393:410	human serum IgG Fc	393:410	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	0	90	theme	N-glycan	13:20	arg1	sialylation					22:32	Fc N-glycan sialylation	10:32	Fc N-glycan sialylation	10:32	Impact of Fc N-glycan sialylation on IgG structure.					
31411531	1	91	from	acid	117:120	arg1	N-glycans					134:142	their Fc N-glycans	125:142	their Fc N-glycans	125:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	1	92	theme	alpha	93:97	arg1	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	6	93	theme	CHO-expressed	1059:1071	arg1	IgG					1073:1075	CHO-expressed IgG	1059:1075	CHO-expressed IgG	1059:1075	When human-derived and CHO-expressed IgG1 were analyzed by HDX, sialic acid-containing glycans were found to destabilize the CH2 domain in CHO-expressed IgG, but not human-derived IgG.					
31411531	7	94	dep	6-arm	1294:1298	arg1	glycans					1322:1328	the major sialylated glycans	1301:1328	the major sialylated glycans in CHO-expressed IgG1	1301:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	3	95	theme	hamster	491:497	arg1	cells					511:515	Chinese hamster ovary (CHO) cells	483:515	Chinese hamster ovary (CHO) cells	483:515	Recombinant IgGs expressed in Chinese hamster ovary (CHO) cells, however, have sialic acids through alpha 2,3-linkages because of the lack of the alpha 2,6-sialyltransferase gene.					
31411531	7	96	theme	glycans	1143:1149	arg1	isomers					1121:1127	structural isomers	1110:1127	structural isomers of sialylated glycans	1110:1149	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	8	97	theme	2,3-linked	1584:1593	arg1	acid					1602:1605	the alpha 2,3-linked sialic acid	1574:1605	the alpha 2,3-linked sialic acid on the 3-arm	1574:1618	The alpha 2,6-linked sialic acid on the 3-arm (the major sialylated glycan in human-derived IgG), and the alpha 2,3-linked sialic acid on the 3-arm, do not have this destabilizing effect.					
31411531	1	98	contain	possess	220:226	arg1	antibodies					62:71	Human IgG antibodies	52:71	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans	52:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	1	98	contain	possess	220:226	arg2	properties					246:255	anti-inflammatory properties	228:255	anti-inflammatory properties	228:255	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
31411531	5	99	dep	exchange	868:875	arg1	experiments					907:917	experiments	907:917	experiments	907:917	In this work, we investigated the impact of different types of sialylation to the conformational stability of IgG through hydrogen/deuterium exchange (HDX) and limited proteolysis experiments.					
31411531	2	100	theme	alpha	358:362	arg1	2,6-linkage					364:374	an alpha 2,6-linkage	355:374	an alpha 2,6-linkage	355:374	Although terminal sialylation on complex N-glycans can happen via either an alpha 2,3-linkage or an alpha 2,6-linkage, sialic acids on human serum IgG Fc are almost exclusively alpha 2,6-linked.					
31411531	7	101	from	glycans	1322:1328	arg1	IgG1					1347:1350	CHO-expressed IgG1	1333:1350	CHO-expressed IgG1	1333:1350	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	7	102	theme	sialic	1275:1280	arg1	acid					1282:1285	only alpha 2,3-linked sialic acid	1253:1285	only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1)	1253:1351	When structural isomers of sialylated glycans were chromatographically resolved and identified in the limited proteolysis experiment, we found that only alpha 2,3-linked sialic acid on the 6-arm (the major sialylated glycans in CHO-expressed IgG1) destabilizes the CH2 domain, presumably because of the steric effect that decreases the glycan-CH2 domain interaction.					
31411531	1	103	link	2,6-linked	99:108	arg1	acid					117:120	terminal alpha 2,6-linked sialic acid	84:120	terminal alpha 2,6-linked sialic acid on their Fc N-glycans	84:142	Human IgG antibodies containing terminal alpha 2,6-linked sialic acid on their Fc N-glycans have been shown to reduce antibody-dependent cell-mediated cytotoxicity and possess anti-inflammatory properties.					
30935958	12	0	theme	organic	1455:1461	arg1	chemistry					1473:1481	organic synthetic chemistry	1455:1481	organic synthetic chemistry	1455:1481	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	8	1	theme	aggregate	1017:1025	arg1	phase					1027:1031	The aggregate phase	1013:1031	The aggregate phase	1013:1031	The aggregate phase is largely circumvented by seeding with preformed PrP fibrils.					
30935958	5	2	theme	PrP	682:684	arg1	formation					693:701	PrP fibril formation	682:701	PrP fibril formation under native conditions in vitro	682:734	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	10	3	theme	PrP	1236:1238	arg1	stability					1240:1248	PrP stability	1236:1248	PrP stability	1236:1248	Little is known about the importance of these positions and their glycans for PrP stability, aggregation and fibril formation.					
30935958	2	4	theme	two-stranded	385:396	arg1	β-sheet					398:404	a small two-stranded β-sheet	377:404	a small two-stranded β-sheet	377:404	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	1	5	theme	neurodegenerative	158:174	arg1	disease					244:250	Creutzfeldt-Jakob disease	226:250	Creutzfeldt-Jakob disease	226:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	5	theme	neurodegenerative	158:174	arg1	encephalopathies					201:216	neurodegenerative transmissible spongiform encephalopathies	158:216	neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease	158:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	15	6	theme	fibril	1973:1978	arg1	formation					1980:1988	fibril formation	1973:1988	fibril formation	1973:1988	As expected, synthetic glycosylated 197 is less affected towards fibril formation compared to glycosylated 181.					
30935958	1	7	theme	spongiform	190:199	arg1	disease					244:250	Creutzfeldt-Jakob disease	226:250	Creutzfeldt-Jakob disease	226:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	7	theme	spongiform	190:199	arg1	encephalopathies					201:216	neurodegenerative transmissible spongiform encephalopathies	158:216	neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease	158:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	4	8	theme	infectious	659:668	arg1	PrP					670:672	misfolded infectious PrP	649:672	misfolded infectious PrP	649:672	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	15	9	theme	glycosylated	1931:1942	arg1	197					1944:1946	synthetic glycosylated 197	1921:1946	synthetic glycosylated 197	1921:1946	As expected, synthetic glycosylated 197 is less affected towards fibril formation compared to glycosylated 181.					
30935958	4	10	dep	residues	583:590	arg1	is					603:604	is	603:604	is	603:604	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	5	11	theme	initial	827:833	arg1	aggregation					835:845	initial aggregation	827:845	initial aggregation forming morphologically disordered aggregates	827:891	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	1	12	theme	Creutzfeldt-Jakob	226:242	arg1	disease					244:250	Creutzfeldt-Jakob disease	226:250	Creutzfeldt-Jakob disease	226:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	17	13	theme	key	2262:2264	arg1	glycosylation					2227:2239	glycosylation	2227:2239	glycosylation of position 181	2227:2255	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	17	13	theme	key	2262:2264	arg1	site					2266:2269	a key site	2260:2269	a key site for prion strain differentiation in vivo	2260:2310	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	5	14	theme	native	709:714	arg1	conditions					716:725	native conditions	709:725	native conditions	709:725	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	14	15	theme	amyloid	1891:1897	arg1	fibrils					1899:1905	amyloid fibrils	1891:1905	amyloid fibrils	1891:1905	Position 197 is less involved in the aggregate trapping and is more geared towards β-sheet structure conversion within amyloid fibrils.					
30935958	0	16	theme	N-glycosylation	10:24	arg1	variants					31:38	N-glycosylation site variants	10:38	N-glycosylation site variants	10:38	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	4	17	theme	truncated	510:518	arg1	PrP					520:522	truncated PrP	510:522	truncated PrP (residues 90-231)	510:540	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	4	17	theme	truncated	510:518	arg1	residues					525:532	residues 90-231	525:539	residues 90-231	525:539	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	0	18	theme	fibril	73:78	arg1	nucleation					80:89	fibril nucleation	73:89	fibril nucleation	73:89	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	8	19	theme	preformed	1073:1081	arg1	fibrils					1087:1093	preformed PrP fibrils	1073:1093	preformed PrP fibrils	1073:1093	The aggregate phase is largely circumvented by seeding with preformed PrP fibrils.					
30935958	14	20	theme	β-sheet	1855:1861	arg1	conversion					1873:1882	β-sheet structure conversion	1855:1882	β-sheet structure conversion within amyloid fibrils	1855:1905	Position 197 is less involved in the aggregate trapping and is more geared towards β-sheet structure conversion within amyloid fibrils.					
30935958	13	21	theme	amyloid	1746:1752	arg1	formation					1761:1769	amyloid fibril formation	1746:1769	amyloid fibril formation	1746:1769	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	5	22	theme	disordered	741:750	arg1	domain					763:768	the disordered N-terminal domain	737:768	the disordered N-terminal domain	737:768	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	7	23	theme	initial	994:1000	arg1	aggregate					1002:1010	this initial aggregate	989:1010	this initial aggregate	989:1010	Nucleation of fibrils occurs from this initial aggregate.					
30935958	1	24	theme	prion	132:136	arg1	PrP					147:149	PrP	147:149	PrP	147:149	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	24	theme	prion	132:136	arg1	protein					138:144	human prion protein	126:144	the human prion protein (PrP)	122:150	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	13	25	theme	initial	1686:1692	arg1	aggregate					1694:1702	the initial aggregate	1682:1702	the initial aggregate preventing conformational changes towards amyloid fibril formation	1682:1769	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	17	26	theme	position	2244:2251	arg1	glycosylation					2227:2239	glycosylation	2227:2239	glycosylation of position 181	2227:2255	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	17	26	theme	position	2244:2251	arg1	site					2266:2269	a key site	2260:2269	a key site for prion strain differentiation in vivo	2260:2310	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	13	27	from	trap	1674:1677	arg1	aggregate					1694:1702	the initial aggregate	1682:1702	the initial aggregate preventing conformational changes towards amyloid fibril formation	1682:1769	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	3	28	theme	N-terminal	431:440	arg1	residues					450:457	residues 23-124	450:464	residues 23-124	450:464	The N-terminal domain (residues 23-124) is intrinsically disordered.					
30935958	3	28	theme	N-terminal	431:440	arg1	domain					442:447	The N-terminal domain	427:447	The N-terminal domain (residues 23-124)	427:465	The N-terminal domain (residues 23-124) is intrinsically disordered.					
30935958	7	29	theme	fibrils	969:975	arg1	Nucleation					955:964	Nucleation	955:964	Nucleation of fibrils	955:975	Nucleation of fibrils occurs from this initial aggregate.					
30935958	16	30	theme	intermolecular	2081:2094	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	2	31	dep	residues	348:355	arg1	125-209					357:363	125-209	357:363	125-209	357:363	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	5	32	theme	aggregation	835:845	arg1	mechanism					814:822	a mechanism	812:822	a mechanism of initial aggregation forming morphologically disordered aggregates	812:891	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	9	33	gly	N-glycosylated	1111:1124	arg2	Asn181					1139:1144	Asn181	1139:1144	Asn181	1139:1144	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	9	33	gly	N-glycosylated	1111:1124	arg2	Asn197					1150:1155	Asn197	1150:1155	Asn197	1150:1155	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	9	33	gly	N-glycosylated	1111:1124	arg1	PrP					1104:1106	In vivo PrP	1096:1106	In vivo PrP	1096:1106	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	9	33	gly	N-glycosylated	1111:1124	arg2	positions					1129:1137	positions	1129:1137	positions Asn181 and Asn197	1129:1155	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	6	34	theme	disordered	912:921	arg1	aggregate					923:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate is a transient phase.					
30935958	6	34	theme	disordered	912:921	arg1	phase					948:952	a transient phase	936:952	a transient phase	936:952	The morphological disordered aggregate is a transient phase.					
30935958	4	35	theme	prion	565:569	arg1	disease					571:577	prion disease	565:577	prion disease	565:577	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	16	36	theme	model	2104:2108	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	0	37	theme	variants	31:38	arg1	Impact					0:5	Impact	0:5	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.	0:90	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	4	38	dep	residues	525:532	arg1	90-231					534:539	90-231	534:539	90-231	534:539	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	5	39	theme	due	805:807	arg1	formation					788:796	fibril formation	781:796	fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates	781:891	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	9	40	dep	positions	1129:1137	arg1	positions					1129:1137	positions	1129:1137	positions Asn181 and Asn197	1129:1155	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	9	40	dep	positions	1129:1137	arg1	Asn197					1150:1155	Asn197	1150:1155	Asn197	1150:1155	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	9	40	dep	positions	1129:1137	arg1	Asn181					1139:1144	Asn181	1139:1144	Asn181	1139:1144	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	10	41	theme	positions	1204:1212	arg1	glycans					1224:1230	their glycans	1218:1230	their glycans for PrP stability, aggregation and fibril formation	1218:1282	Little is known about the importance of these positions and their glycans for PrP stability, aggregation and fibril formation.					
30935958	10	41	theme	positions	1204:1212	arg1	importance					1184:1193	the importance	1180:1193	the importance of these positions	1180:1212	Little is known about the importance of these positions and their glycans for PrP stability, aggregation and fibril formation.					
30935958	0	42	theme	human	47:51	arg1	aggregation					57:67	human PrP aggregation	47:67	human PrP aggregation	47:67	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	14	43	theme	aggregate	1809:1817	arg1	trapping					1819:1826	the aggregate trapping	1805:1826	the aggregate trapping	1805:1826	Position 197 is less involved in the aggregate trapping and is more geared towards β-sheet structure conversion within amyloid fibrils.					
30935958	3	44	dep	residues	450:457	arg1	23-124					459:464	23-124	459:464	23-124	459:464	The N-terminal domain (residues 23-124) is intrinsically disordered.					
30935958	16	45	theme	PrP90-231	2127:2135	arg1	fibril					2137:2142	the PrP90-231 fibril	2123:2142	the PrP90-231 fibril	2123:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	9	46	from	positions	1129:1137	arg1	N-glycosylated					1111:1124	N-glycosylated	1111:1124	N-glycosylated	1111:1124	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	12	47	theme	synthetic	1519:1527	arg1	glycosylations					1529:1542	generated synthetic glycosylations	1509:1542	generated synthetic glycosylations	1509:1542	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	11	48	theme	positional	1398:1407	arg1	impact					1409:1414	the positional impact	1394:1414	the positional impact on these processes	1394:1433	We have in this study taken a step towards that goal by mutating residues 181 and 197 for cysteines to study the positional impact on these processes.					
30935958	12	49	theme	synthetic	1463:1471	arg1	chemistry					1473:1481	organic synthetic chemistry	1455:1481	organic synthetic chemistry	1455:1481	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	4	50	theme	amyloid-like	621:632	arg1	core					641:644	the amyloid-like fibril core	617:644	the amyloid-like fibril core of misfolded infectious PrP	617:672	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	17	51	theme	prion	2275:2279	arg1	differentiation					2288:2302	prion strain differentiation	2275:2302	prion strain differentiation in vivo	2275:2310	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	11	52	dep	residues	1350:1357	arg1	181					1359:1361	181	1359:1361	181	1359:1361	We have in this study taken a step towards that goal by mutating residues 181 and 197 for cysteines to study the positional impact on these processes.					
30935958	11	52	dep	residues	1350:1357	arg1	197					1367:1369	197	1367:1369	197	1367:1369	We have in this study taken a step towards that goal by mutating residues 181 and 197 for cysteines to study the positional impact on these processes.					
30935958	6	53	theme	transient	938:946	arg1	aggregate					923:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate is a transient phase.					
30935958	6	53	theme	transient	938:946	arg1	phase					948:952	a transient phase	936:952	a transient phase	936:952	The morphological disordered aggregate is a transient phase.					
30935958	16	54	with	compatible	2040:2049	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	13	55	theme	key	1628:1630	arg1	residue					1584:1590	residue 181	1584:1594	residue 181 when mutated to a cysteine	1584:1621	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	13	55	theme	key	1628:1630	arg1	residue					1632:1638	a key residue	1626:1638	a key residue for self-chaperoning	1626:1659	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	4	56	theme	PrP	670:672	arg1	core					641:644	the amyloid-like fibril core	617:644	the amyloid-like fibril core of misfolded infectious PrP	617:672	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	16	57	theme	PrP	2193:2195	arg1	transitions					2178:2188	the misfolding transitions	2163:2188	the misfolding transitions of PrP	2163:2195	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	16	58	theme	misfolding	2167:2176	arg1	transitions					2178:2188	the misfolding transitions	2163:2188	the misfolding transitions of PrP	2163:2195	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	2	59	theme	C-terminal	320:329	arg1	residues					348:355	residues 125-209	348:363	residues 125-209	348:363	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	2	59	theme	C-terminal	320:329	arg1	domain					340:345	a C-terminal globular domain	318:345	a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices	318:424	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	1	60	theme	transmissible	176:188	arg1	disease					244:250	Creutzfeldt-Jakob disease	226:250	Creutzfeldt-Jakob disease	226:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	60	theme	transmissible	176:188	arg1	encephalopathies					201:216	neurodegenerative transmissible spongiform encephalopathies	158:216	neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease	158:250	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	9	61	dep	In	1096:1097	arg1	vivo					1099:1102	vivo	1099:1102	vivo	1099:1102	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	15	62	theme	synthetic	1921:1929	arg1	197					1944:1946	synthetic glycosylated 197	1921:1946	synthetic glycosylated 197	1921:1946	As expected, synthetic glycosylated 197 is less affected towards fibril formation compared to glycosylated 181.					
30935958	15	63	gly	glycosylated	1931:1942	arg1	197					1944:1946	synthetic glycosylated 197	1921:1946	synthetic glycosylated 197	1921:1946	As expected, synthetic glycosylated 197 is less affected towards fibril formation compared to glycosylated 181.					
30935958	5	64	theme	disordered	871:880	arg1	aggregates					882:891	morphologically disordered aggregates	855:891	morphologically disordered aggregates	855:891	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	4	65	theme	misfolded	649:657	arg1	PrP					670:672	misfolded infectious PrP	649:672	misfolded infectious PrP	649:672	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	12	66	dep	chemistry	1473:1481	arg1	glycosylations					1529:1542	generated synthetic glycosylations	1509:1542	generated synthetic glycosylations	1509:1542	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	5	67	theme	fibril	686:691	arg1	formation					693:701	PrP fibril formation	682:701	PrP fibril formation under native conditions in vitro	682:734	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	1	68	theme	human	126:130	arg1	PrP					147:149	PrP	147:149	PrP	147:149	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	68	theme	human	126:130	arg1	protein					138:144	human prion protein	126:144	the human prion protein (PrP)	122:150	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	10	69	theme	fibril	1267:1272	arg1	formation					1274:1282	fibril formation	1267:1282	fibril formation	1267:1282	Little is known about the importance of these positions and their glycans for PrP stability, aggregation and fibril formation.					
30935958	11	70	from	impact	1409:1414	arg1	processes					1425:1433	these processes	1419:1433	these processes	1419:1433	We have in this study taken a step towards that goal by mutating residues 181 and 197 for cysteines to study the positional impact on these processes.					
30935958	1	71	theme	protein	138:144	arg1	Misfolding					92:101	Misfolding	92:101	Misfolding	92:101	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	1	71	theme	protein	138:144	arg1	aggregation					107:117	aggregation	107:117	aggregation	107:117	Misfolding and aggregation of the human prion protein (PrP) cause neurodegenerative transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease.					
30935958	17	72	gly	glycosylation	2227:2239	arg1	position					2244:2251	position 181	2244:2255	position 181	2244:2255	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	4	73	theme	PrP	520:522	arg1	Expression					496:505	Expression	496:505	Expression of truncated PrP (residues 90-231)	496:540	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
30935958	9	74	theme	In	1096:1097	arg1	PrP					1104:1106	In vivo PrP	1096:1106	In vivo PrP	1096:1106	In vivo PrP is N-glycosylated at positions Asn181 and Asn197.					
30935958	5	75	theme	fibril	781:786	arg1	formation					788:796	fibril formation	781:796	fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates	781:891	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	8	76	theme	PrP	1083:1085	arg1	fibrils					1087:1093	preformed PrP fibrils	1073:1093	preformed PrP fibrils	1073:1093	The aggregate phase is largely circumvented by seeding with preformed PrP fibrils.					
30935958	14	77	theme	structure	1863:1871	arg1	conversion					1873:1882	β-sheet structure conversion	1855:1882	β-sheet structure conversion within amyloid fibrils	1855:1905	Position 197 is less involved in the aggregate trapping and is more geared towards β-sheet structure conversion within amyloid fibrils.					
30935958	17	78	theme	strain	2281:2286	arg1	differentiation					2288:2302	prion strain differentiation	2275:2302	prion strain differentiation in vivo	2275:2310	We hypothesize that glycosylation of position 181 is a key site for prion strain differentiation in vivo.					
30935958	16	79	theme	fibril	2137:2142	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	13	80	theme	fibril	1754:1759	arg1	formation					1761:1769	amyloid fibril formation	1746:1769	amyloid fibril formation	1746:1769	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	5	81	theme	N-terminal	752:761	arg1	domain					763:768	the disordered N-terminal domain	737:768	the disordered N-terminal domain	737:768	During PrP fibril formation under native conditions in vitro, the disordered N-terminal domain slows down fibril formation likely due to a mechanism of initial aggregation forming morphologically disordered aggregates.					
30935958	13	82	theme	conformational	1715:1728	arg1	changes					1730:1736	conformational changes	1715:1736	conformational changes	1715:1736	Our data shows that residue 181 when mutated to a cysteine is a key residue for self-chaperoning, rendering a trap in the initial aggregate preventing conformational changes towards amyloid fibril formation.					
30935958	2	83	theme	small	379:383	arg1	β-sheet					398:404	a small two-stranded β-sheet	377:404	a small two-stranded β-sheet	377:404	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	0	84	theme	site	26:29	arg1	variants					31:38	N-glycosylation site variants	10:38	N-glycosylation site variants	10:38	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	16	85	theme	parallel	2060:2067	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	2	86	theme	native	260:265	arg1	PrP					267:269	Mature native PrP	253:269	Mature native PrP	253:269	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	16	87	theme	in-register	2069:2079	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	6	88	theme	morphological	898:910	arg1	aggregate					923:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate	894:931	The morphological disordered aggregate is a transient phase.					
30935958	6	88	theme	morphological	898:910	arg1	phase					948:952	a transient phase	936:952	a transient phase	936:952	The morphological disordered aggregate is a transient phase.					
30935958	16	89	theme	β-sheet	2096:2102	arg1	structure					2110:2118	the parallel in-register intermolecular β-sheet model structure	2056:2118	the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril	2056:2142	Our data are rather compatible with the parallel in-register intermolecular β-sheet model structure of the PrP90-231 fibril and sheds light on the misfolding transitions of PrP in vitro.					
30935958	0	90	theme	PrP	53:55	arg1	aggregation					57:67	human PrP aggregation	47:67	human PrP aggregation	47:67	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	0	91	gly	N-glycosylation	10:24	arg2	site					26:29	N-glycosylation site variants	10:38	N-glycosylation site variants	10:38	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	0	91	gly	N-glycosylation	10:24	arg2	variants					31:38	N-glycosylation site variants	10:38	N-glycosylation site variants	10:38	Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation.					
30935958	12	92	theme	chemical	1487:1494	arg1	modification					1496:1507	chemical modification	1487:1507	chemical modification	1487:1507	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	2	93	theme	Mature	253:258	arg1	PrP					267:269	Mature native PrP	253:269	Mature native PrP	253:269	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	2	94	theme	globular	331:338	arg1	residues					348:355	residues 125-209	348:363	residues 125-209	348:363	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	2	94	theme	globular	331:338	arg1	domain					340:345	a C-terminal globular domain	318:345	a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices	318:424	Mature native PrP is composed of 209 residues and is folded into a C-terminal globular domain (residues 125-209) comprising a small two-stranded β-sheet and three α-helices.					
30935958	12	95	theme	generated	1509:1517	arg1	glycosylations					1529:1542	generated synthetic glycosylations	1509:1542	generated synthetic glycosylations	1509:1542	We have further by organic synthetic chemistry and chemical modification generated synthetic glycosylations in these positions.					
30935958	4	96	theme	fibril	634:639	arg1	core					641:644	the amyloid-like fibril core	617:644	the amyloid-like fibril core of misfolded infectious PrP	617:672	Expression of truncated PrP (residues 90-231) is sufficient to cause prion disease and residues 90/100-231 is comprising the amyloid-like fibril core of misfolded infectious PrP.					
31499077	6	0	theme	N-glycosylation	1006:1020	arg1	changes					1022:1028	qualitative and quantitative N-glycosylation changes	977:1028	qualitative and quantitative N-glycosylation changes	977:1028	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	12	1	theme	epithelial	1991:2000	arg1	differentiation					2007:2021	intestinal epithelial cell differentiation	1980:2021	intestinal epithelial cell differentiation	1980:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	11	2	dep	together	1736:1743	arg1	with					1745:1748	with	1745:1748	with	1745:1748	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	8	3	theme	individual	1300:1309	arg1	species					1318:1324	individual glycan species	1300:1324	individual glycan species	1300:1324	This was also clearly visible when we analyzed the abundances of individual glycan species.					
31499077	11	4	theme	enhanced	1857:1864	arg1	fucosylation					1866:1877	fucosylation	1866:1877	fucosylation	1866:1877	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	5	theme	N-glycosylation	1688:1702	arg1	pathway					1704:1710	N-glycosylation pathway	1688:1710	N-glycosylation pathway	1688:1710	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	5	6	link	glycosecretome-derived	755:776	arg1	N-glycomes					778:787	the glycoproteome- and glycosecretome-derived N-glycomes	732:787	the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells	732:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	8	7	theme	species	1318:1324	arg1	abundances					1286:1295	the abundances	1282:1295	the abundances of individual glycan species	1282:1324	This was also clearly visible when we analyzed the abundances of individual glycan species.					
31499077	12	8	theme	potential	1957:1965	arg1	glycan					1945:1950	H4N5F1 glycan	1938:1950	H4N5F1 glycan	1938:1950	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	12	8	theme	potential	1957:1965	arg1	biomarker					1967:1975	a potential biomarker	1955:1975	a potential biomarker of intestinal epithelial cell differentiation	1955:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	4	9	theme	differentiation	657:671	arg1	model					632:636	a broadly used in vitro model	608:636	a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details	608:717	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	11	10	theme	N-glycans	1843:1851	arg1	diversity					1830:1838	increased diversity	1820:1838	increased diversity of N-glycans	1820:1851	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	10	theme	N-glycans	1843:1851	arg1	sialylation					1883:1893	sialylation	1883:1893	sialylation	1883:1893	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	10	theme	N-glycans	1843:1851	arg1	fucosylation					1866:1877	fucosylation	1866:1877	fucosylation	1866:1877	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	10	11	theme	Golgi	1568:1572	arg1	alpha-mannosidases					1574:1591	Golgi alpha-mannosidases	1568:1591	Golgi alpha-mannosidases (especially MAN1C1)	1568:1611	This was supported by elevated expression of Golgi alpha-mannosidases (especially MAN1C1).					
31499077	2	12	theme	cellular	319:326	arg1	differentiation					328:342	cellular differentiation	319:342	cellular differentiation	319:342	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	12	13	theme	H4N5F1	1938:1943	arg1	glycan					1945:1950	H4N5F1 glycan	1938:1950	H4N5F1 glycan	1938:1950	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	12	13	theme	H4N5F1	1938:1943	arg1	biomarker					1967:1975	a potential biomarker	1955:1975	a potential biomarker of intestinal epithelial cell differentiation	1955:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	1	14	theme	epithelial	203:212	arg1	differentiation					220:234	epithelial cells differentiation	203:234	epithelial cells differentiation	203:234	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	4	15	theme	intestinal	641:650	arg1	differentiation					657:671	intestinal cell differentiation	641:671	intestinal cell differentiation	641:671	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	5	16	link	Caco-2-derived	841:854	arg1	cells					872:876	Caco-2-derived enterocyte-like cells	841:876	Caco-2-derived enterocyte-like cells	841:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	1	17	theme	differentiation	220:234	arg1	process					192:198	the complex process	180:198	the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life	180:289	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	4	18	dep	in	623:624	arg1	vitro					626:630	vitro	626:630	vitro	626:630	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	10	19	theme	elevated	1545:1552	arg1	expression					1554:1563	elevated expression	1545:1563	elevated expression of Golgi alpha-mannosidases (especially MAN1C1)	1545:1611	This was supported by elevated expression of Golgi alpha-mannosidases (especially MAN1C1).					
31499077	4	20	theme	glycosylation	686:698	arg1	changes					700:706	the glycosylation changes	682:706	the glycosylation changes in details	682:717	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	11	21	theme	mannose	1647:1653	arg1	trimming					1655:1662	intensified mannose trimming	1635:1662	intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases	1635:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	9	22	theme	mannose	1436:1442	arg1	trimming					1444:1451	mannose trimming	1436:1451	mannose trimming	1436:1451	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	1	23	theme	intestinal	147:156	arg1	epithelium					158:167	the intestinal epithelium	143:167	the intestinal epithelium	143:167	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	11	24	gly	sialylation	1883:1893	arg1	structures					1906:1915	complex structures	1898:1915	complex structures	1898:1915	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	24	gly	sialylation	1883:1893	arg1	N-glycans					1843:1851	N-glycans	1843:1851	N-glycans	1843:1851	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	5	25	theme	Caco-2	809:814	arg1	cells					831:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	11	26	theme	initial	1671:1677	arg1	steps					1679:1683	the initial steps	1667:1683	the initial steps of N-glycosylation pathway	1667:1710	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	0	27	theme	epithelial	91:100	arg1	cells					102:106	intestinal epithelial cells	80:106	intestinal epithelial cells	80:106	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	3	28	theme	tract	560:564	arg1	disease					566:572	gastrointestinal tract disease	543:572	gastrointestinal tract disease	543:572	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	7	29	theme	glycan	1060:1065	arg1	analysis					1074:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	5	30	theme	glycosecretome-derived	755:776	arg1	N-glycomes					778:787	the glycoproteome- and glycosecretome-derived N-glycomes	732:787	the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells	732:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	0	31	theme	proteome	31:38	arg1	N-glycosylation					12:26	N-glycosylation	12:26	N-glycosylation of proteome and secretome	12:52	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	5	32	theme	cells	872:876	arg1	N-glycomes					778:787	the glycoproteome- and glycosecretome-derived N-glycomes	732:787	the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells	732:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	6	33	theme	exoglycosidases	938:952	arg1	digestions					954:963	exoglycosidases digestions	938:963	exoglycosidases digestions	938:963	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	11	34	theme	complex	1898:1904	arg1	structures					1906:1915	complex structures	1898:1915	complex structures	1898:1915	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	35	gly	fucosylation	1866:1877	arg1	structures					1906:1915	complex structures	1898:1915	complex structures	1898:1915	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	35	gly	fucosylation	1866:1877	arg1	N-glycans					1843:1851	N-glycans	1843:1851	N-glycans	1843:1851	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	9	36	theme	glycan	1515:1520	arg1	downregulation					1467:1480	downregulation	1467:1480	downregulation of H8N2	1467:1488	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	9	36	theme	glycan	1515:1520	arg1	upregulation					1494:1505	upregulation	1494:1505	upregulation of H5N2 glycan	1494:1520	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	5	37	theme	cells	831:835	arg1	N-glycomes					778:787	the glycoproteome- and glycosecretome-derived N-glycomes	732:787	the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells	732:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	11	38	theme	expression	1772:1781	arg1	remodeling					1754:1763	the remodeling	1750:1763	the remodeling of the expression of key glycosyltransferases	1750:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	5	39	theme	Caco-2-derived	841:854	arg1	cells					872:876	Caco-2-derived enterocyte-like cells	841:876	Caco-2-derived enterocyte-like cells	841:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	6	40	theme	MALDI-ToF-MS	902:913	arg1	approach					915:922	HILIC-HPLC and MALDI-ToF-MS approach	887:922	HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions	887:963	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	0	41	gly	N-glycosylation	12:26	arg1	proteome					31:38	proteome	31:38	proteome	31:38	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	0	41	gly	N-glycosylation	12:26	arg1	secretome					44:52	secretome	44:52	secretome	44:52	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	6	42	theme	HILIC-HPLC	887:896	arg1	approach					915:922	HILIC-HPLC and MALDI-ToF-MS approach	887:922	HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions	887:963	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	12	43	theme	intestinal	1980:1989	arg1	differentiation					2007:2021	intestinal epithelial cell differentiation	1980:2021	intestinal epithelial cell differentiation	1980:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	2	44	theme	latter	407:412	arg1	changes					392:398	at least that changes	378:398	at least that changes of the latter	378:412	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	2	44	theme	latter	407:412	arg1	hallmark					422:429	the hallmark	418:429	the hallmark of the process	418:444	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	10	45	dep	alpha-mannosidases	1574:1591	arg1	MAN1C1					1605:1610	MAN1C1	1605:1610	especially MAN1C1	1594:1610	This was supported by elevated expression of Golgi alpha-mannosidases (especially MAN1C1).					
31499077	12	46	theme	cell	2002:2005	arg1	differentiation					2007:2021	intestinal epithelial cell differentiation	1980:2021	intestinal epithelial cell differentiation	1980:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	4	47	theme	used	618:621	arg1	model					632:636	a broadly used in vitro model	608:636	a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details	608:717	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	5	48	theme	undifferentiated	792:807	arg1	cells					831:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	11	49	theme	key	1786:1788	arg1	glycosyltransferases					1790:1809	key glycosyltransferases	1786:1809	key glycosyltransferases	1786:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	4	50	from	changes	700:706	arg1	details					711:717	details	711:717	details	711:717	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	6	51	theme	quantitative	993:1004	arg1	changes					1022:1028	qualitative and quantitative N-glycosylation changes	977:1028	qualitative and quantitative N-glycosylation changes	977:1028	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	11	52	theme	increased	1820:1828	arg1	diversity					1830:1838	increased diversity	1820:1838	increased diversity of N-glycans	1820:1851	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	3	53	from	important	515:523	arg1	context					532:538	the context	528:538	the context of gastrointestinal tract disease, including cancer	528:590	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	3	54	theme	gastrointestinal	543:558	arg1	disease					566:572	gastrointestinal tract disease	543:572	gastrointestinal tract disease	543:572	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	1	55	theme	entire	279:284	arg1	life					286:289	the entire life	275:289	the entire life	275:289	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	6	56	theme	qualitative	977:987	arg1	changes					1022:1028	qualitative and quantitative N-glycosylation changes	977:1028	qualitative and quantitative N-glycosylation changes	977:1028	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	11	57	theme	pathway	1704:1710	arg1	steps					1679:1683	the initial steps	1667:1683	the initial steps of N-glycosylation pathway	1667:1710	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	2	58	theme	Many	292:295	arg1	studies					297:303	Many studies	292:303	Many studies	292:303	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	8	59	theme	glycan	1311:1316	arg1	species					1318:1324	individual glycan species	1300:1324	individual glycan species	1300:1324	This was also clearly visible when we analyzed the abundances of individual glycan species.					
31499077	4	60	theme	cell	652:655	arg1	differentiation					657:671	intestinal cell differentiation	641:671	intestinal cell differentiation	641:671	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	10	61	theme	alpha-mannosidases	1574:1591	arg1	expression					1554:1563	elevated expression	1545:1563	elevated expression of Golgi alpha-mannosidases (especially MAN1C1)	1545:1611	This was supported by elevated expression of Golgi alpha-mannosidases (especially MAN1C1).					
31499077	1	62	theme	complex	184:190	arg1	process					192:198	the complex process	180:198	the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life	180:289	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	9	63	theme	characteristic	1353:1366	arg1	shift					1368:1372	the characteristic shift	1349:1372	the characteristic shift	1349:1372	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	7	64	theme	fucosylation	1199:1210	arg1	glycoproteome					1168:1180	glycoproteome	1168:1180	glycoproteome	1168:1180	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	7	64	theme	fucosylation	1199:1210	arg1	increment					1186:1194	increment	1186:1194	increment	1186:1194	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	3	65	theme	disease	566:572	arg1	context					532:538	the context	528:538	the context of gastrointestinal tract disease, including cancer	528:590	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	7	66	theme	increment	1186:1194	arg1	sialylation					1153:1163	sialylation	1153:1163	sialylation of glycoproteome and increment of fucosylation within glycosecretome	1153:1232	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	9	67	theme	oligomannose	1381:1392	arg1	N-glycans					1394:1402	oligomannose N-glycans	1381:1402	oligomannose N-glycans	1381:1402	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	1	68	theme	cells	214:218	arg1	differentiation					220:234	epithelial cells differentiation	203:234	epithelial cells differentiation	203:234	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	7	69	theme	glycoproteome	1168:1180	arg1	sialylation					1153:1163	sialylation	1153:1163	sialylation of glycoproteome and increment of fucosylation within glycosecretome	1153:1232	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	7	70	theme	sialylation	1153:1163	arg1	upregulation					1137:1148	substantial upregulation	1125:1148	substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome	1125:1232	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	7	71	theme	substantial	1125:1135	arg1	upregulation					1137:1148	substantial upregulation	1125:1148	substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome	1125:1232	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	1	72	theme	homeostasis	128:138	arg1	maintenance					113:123	The maintenance	109:123	The maintenance of homeostasis of the intestinal epithelium	109:167	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	7	73	gly	sialylation	1153:1163	arg1	glycoproteome					1168:1180	glycoproteome	1168:1180	glycoproteome	1168:1180	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	7	73	gly	sialylation	1153:1163	arg1	increment					1186:1194	increment	1186:1194	increment	1186:1194	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	11	74	theme	intensified	1635:1645	arg1	trimming					1655:1662	intensified mannose trimming	1635:1662	intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases	1635:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	3	75	from	context	532:538	arg1	important					515:523	important	515:523	important	515:523	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	5	76	theme	glycoproteome-	736:749	arg1	N-glycomes					778:787	the glycoproteome- and glycosecretome-derived N-glycomes	732:787	the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells	732:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	0	77	theme	intestinal	80:89	arg1	cells					102:106	intestinal epithelial cells	80:106	intestinal epithelial cells	80:106	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	9	78	theme	trimming	1444:1451	arg1	augmentation					1420:1431	the augmentation	1416:1431	the augmentation	1416:1431	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	1	79	theme	epithelium	158:167	arg1	homeostasis					128:138	homeostasis	128:138	homeostasis of the intestinal epithelium	128:167	The maintenance of homeostasis of the intestinal epithelium depends on the complex process of epithelial cells differentiation, which repeatedly continues throughout the entire life.					
31499077	0	80	theme	cells	102:106	arg1	differentiation					61:75	differentiation	61:75	differentiation of intestinal epithelial cells	61:106	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	7	81	theme	traits	1067:1072	arg1	analysis					1074:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	11	82	from	steps	1679:1683	arg1	trimming					1655:1662	intensified mannose trimming	1635:1662	intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases	1635:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	6	83	used	used	882:885	arg2	We					879:880	We	879:880	We	879:880	We used HILIC-HPLC and MALDI-ToF-MS approach together with exoglycosidases digestions to describe qualitative and quantitative N-glycosylation changes upon differentiation.					
31499077	9	84	theme	H8N2	1485:1488	arg1	downregulation					1467:1480	downregulation	1467:1480	downregulation of H8N2	1467:1488	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	9	84	theme	H8N2	1485:1488	arg1	upregulation					1494:1505	upregulation	1494:1505	upregulation of H5N2 glycan	1494:1520	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	7	85	theme	Derived	1052:1058	arg1	analysis					1074:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis	1052:1081	Derived glycan traits analysis revealed, that differentiation results in substantial upregulation of sialylation of glycoproteome and increment of fucosylation within glycosecretome.					
31499077	2	86	theme	process	438:444	arg1	changes					392:398	at least that changes	378:398	at least that changes of the latter	378:412	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	2	86	theme	process	438:444	arg1	hallmark					422:429	the hallmark	418:429	the hallmark of the process	418:444	Many studies suggest, that cellular differentiation is regulated by glycosylation, or at least that changes of the latter are the hallmark of the process.					
31499077	0	87	theme	N-glycosylation	12:26	arg1	switch					2:7	A switch	0:7	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells	0:106	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	5	88	theme	adenocarcinoma	816:829	arg1	cells					831:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	undifferentiated Caco-2 adenocarcinoma cells	792:835	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
31499077	9	89	theme	H5N2	1510:1513	arg1	glycan					1515:1520	H5N2 glycan	1510:1520	H5N2 glycan	1510:1520	Moreover, we observed the characteristic shift within oligomannose N-glycans, suggesting the augmentation of mannose trimming, resulting in downregulation of H8N2 and upregulation of H5N2 glycan.					
31499077	3	90	theme	detailed	451:458	arg1	description					460:470	description	460:470	description	460:470	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	0	91	theme	secretome	44:52	arg1	N-glycosylation					12:26	N-glycosylation	12:26	N-glycosylation of proteome and secretome	12:52	A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells.					
31499077	12	92	theme	differentiation	2007:2021	arg1	glycan					1945:1950	H4N5F1 glycan	1938:1950	H4N5F1 glycan	1938:1950	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	12	92	theme	differentiation	2007:2021	arg1	biomarker					1967:1975	a potential biomarker	1955:1975	a potential biomarker of intestinal epithelial cell differentiation	1955:2021	Finally, we propose H4N5F1 glycan as a potential biomarker of intestinal epithelial cell differentiation.					
31499077	3	93	theme	relationship	498:509	arg1	understanding					476:488	understanding	476:488	understanding	476:488	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	3	93	theme	relationship	498:509	arg1	description					460:470	description	460:470	description	460:470	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	11	94	theme	structures	1906:1915	arg1	diversity					1830:1838	increased diversity	1820:1838	increased diversity of N-glycans	1820:1851	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	94	theme	structures	1906:1915	arg1	sialylation					1883:1893	sialylation	1883:1893	sialylation	1883:1893	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	94	theme	structures	1906:1915	arg1	fucosylation					1866:1877	fucosylation	1866:1877	fucosylation	1866:1877	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	11	95	theme	glycosyltransferases	1790:1809	arg1	expression					1772:1781	the expression	1768:1781	the expression of key glycosyltransferases	1768:1809	We hypothesize, that intensified mannose trimming at the initial steps of N-glycosylation pathway during differentiation, together with the remodeling of the expression of key glycosyltransferases leads to increased diversity of N-glycans and enhanced fucosylation and sialylation of complex structures.					
31499077	3	96	dep	description	460:470	arg1	The					447:449	The	447:449	The	447:449	The detailed description and understanding of this relationship are important in the context of gastrointestinal tract disease, including cancer.					
31499077	4	97	theme	in	623:624	arg1	model					632:636	a broadly used in vitro model	608:636	a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details	608:717	Here we employ a broadly used in vitro model of intestinal cell differentiation to track the glycosylation changes in details.					
31499077	5	98	theme	enterocyte-like	856:870	arg1	cells					872:876	Caco-2-derived enterocyte-like cells	841:876	Caco-2-derived enterocyte-like cells	841:876	We analyzed the glycoproteome- and glycosecretome-derived N-glycomes of undifferentiated Caco-2 adenocarcinoma cells and Caco-2-derived enterocyte-like cells.					
30525457	9	0	theme	bisected	1786:1793	arg1	N-glycans					1807:1815	agalactosylated bisected biantennary N-glycans	1770:1815	agalactosylated bisected biantennary N-glycans	1770:1815	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	10	1	theme	biantennary	2047:2057	arg1	N-glycan					2059:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	3	2	theme	physiological	572:584	arg1	processes					586:594	physiological processes	572:594	physiological processes	572:594	Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiological processes.					
30525457	9	3	theme	PHA-E	1547:1551	arg1	lectin					1553:1558	PHA-E lectin	1547:1558	PHA-E lectin	1547:1558	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	8	4	theme	aurantia	1300:1307	arg1	lectin					1309:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	7	5	theme	N-glycan	1237:1244	arg1	isomers					1195:1201	the positional isomers	1180:1201	the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan	1180:1289	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	6	6	theme	lectin	963:968	arg1	specificity					970:980	the lectin specificity	959:980	the lectin specificity	959:980	When the lectin specificity matches the N-glycan, the peak disappears from the electropherogram, identifying the N-glycan structure.					
30525457	7	7	dep	monogalactosylated	1206:1223	arg1	biantennary					1225:1235	biantennary	1225:1235	biantennary	1225:1235	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	7	8	theme	monogalactosylated	1206:1223	arg1	N-glycan					1237:1244	monogalactosylated biantennary N-glycan	1206:1244	monogalactosylated biantennary N-glycan	1206:1244	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	9	9	contain	has	1560:1562	arg2	affinity					1581:1588	a strong binding affinity	1564:1588	a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch	1564:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	9	9	contain	has	1560:1562	arg1	lectin					1553:1558	PHA-E lectin	1547:1558	PHA-E lectin	1547:1558	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	2	10	theme	structures	299:308	arg1	possible					314:321	possible	314:321	possible	314:321	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	2	10	theme	structures	299:308	arg1	number					276:281	only a limited number	261:281	only a limited number of IgG N-glycan structures	261:308	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	4	11	from	differences	724:734	arg1	N-glycans					786:794	N-glycans	786:794	N-glycans derived from different sources of human IgG	786:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	4	11	from	differences	724:734	arg1	galactosylation					750:764	galactosylation	750:764	galactosylation	750:764	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	4	11	from	differences	724:734	arg1	sialylation					771:781	sialylation	771:781	sialylation	771:781	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	4	11	from	differences	724:734	arg1	bisection					739:747	bisection	739:747	bisection	739:747	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	9	12	theme	strong	1566:1571	arg1	affinity					1581:1588	a strong binding affinity	1564:1588	a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch	1564:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	8	13	theme	cristagalli	1327:1337	arg1	lectin					1339:1344	Erythrina cristagalli lectin	1317:1344	Erythrina cristagalli lectin (ECL)	1317:1350	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	13	theme	cristagalli	1327:1337	arg1	ECL					1347:1349	ECL	1347:1349	ECL	1347:1349	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	10	14	located	observed	1856:1863	arg2	affinity					1828:1835	The lower affinity	1818:1835	The lower affinity to these motifs	1818:1851	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	10	14	located	observed	1856:1863	arg1	electropherograms					1872:1888	the electropherograms	1868:1888	the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan	1868:2066	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	9	15	contain	containing	1732:1741	arg1	N-glycans					1722:1730	N-glycans	1722:1730	N-glycans containing terminal galactose	1722:1760	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	9	15	contain	containing	1732:1741	arg2	galactose					1752:1760	terminal galactose	1743:1760	terminal galactose	1743:1760	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	8	16	link	α2-6-linked	1465:1475	arg1	acid					1484:1487	α2-6-linked sialic acid	1465:1487	α2-6-linked sialic acid	1465:1487	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	17	theme	vulgaris	1390:1397	arg1	PHA-E					1418:1422	PHA-E	1418:1422	PHA-E	1418:1422	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	17	theme	vulgaris	1390:1397	arg1	Erythroagglutinin					1399:1415	Phaseolus vulgaris Erythroagglutinin	1380:1415	Phaseolus vulgaris Erythroagglutinin (PHA-E)	1380:1423	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	2	18	theme	human	324:328	arg1	N-glycans					334:342	human IgG N-glycans	324:342	human IgG N-glycans	324:342	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	1	19	theme	mannose	238:244	arg1	core					246:249	the mannose core	234:249	the mannose core	234:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	10	20	with	conjunction	1936:1946	arg1	results					1957:1963	the results	1953:1963	the results from the ECL lectin	1953:1983	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	8	21	used	used	1429:1432	arg2	PHA-E					1418:1422	PHA-E	1418:1422	PHA-E	1418:1422	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	21	used	used	1429:1432	arg2	Erythroagglutinin					1399:1415	Phaseolus vulgaris Erythroagglutinin	1380:1415	Phaseolus vulgaris Erythroagglutinin (PHA-E)	1380:1423	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	21	used	used	1429:1432	arg2	lectin					1368:1373	Sambucus nigra lectin	1353:1373	Sambucus nigra lectin	1353:1373	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	21	used	used	1429:1432	arg2	lectin					1339:1344	Erythrina cristagalli lectin	1317:1344	Erythrina cristagalli lectin (ECL)	1317:1350	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	21	used	used	1429:1432	arg2	lectin					1309:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	21	used	used	1429:1432	arg2	ECL					1347:1349	ECL	1347:1349	ECL	1347:1349	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	5	22	theme	stationary	927:936	arg1	zone					938:941	a single stationary zone	918:941	a single stationary zone of lectin	918:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	8	23	theme	Sambucus	1353:1360	arg1	lectin					1368:1373	Sambucus nigra lectin	1353:1373	Sambucus nigra lectin	1353:1373	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	11	24	theme	standard	2191:2198	arg1	deviation					2200:2208	relative standard deviation	2182:2208	less than or equal to 0.08 and 4% relative standard deviation	2148:2208	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	4	25	attach	derived	796:802	arg1	sources					819:825	different sources	809:825	different sources of human IgG	809:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	4	25	attach	derived	796:802	arg2	N-glycans					786:794	N-glycans	786:794	N-glycans derived from different sources of human IgG	786:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	7	26	theme	500 000	1152:1158	arg1	plates					1160:1165	500 000 plates	1152:1165	500 000 plates	1152:1165	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	1	27	theme	fucosylated	126:136	arg1	N-glycans					150:158	fucosylated biantennary N-glycans	126:158	fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core	126:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	10	28	from	change	1895:1900	arg1	width					1910:1914	peak width	1905:1914	peak width	1905:1914	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	11	29	theme	migration	2115:2123	arg1	time					2125:2128	migration time	2115:2128	migration time	2115:2128	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	9	30	theme	lower	1703:1707	arg1	affinity					1709:1716	lower affinity	1703:1716	lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans	1703:1815	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	4	31	used	used	643:646	arg2	method					633:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	2	32	theme	IgG	286:288	arg1	structures					299:308	IgG N-glycan structures	286:308	IgG N-glycan structures	286:308	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	9	33	theme	terminal	1633:1640	arg1	galactose					1642:1650	a terminal galactose	1631:1650	a terminal galactose on the α1-6-linked mannose branch	1631:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	1	34	theme	biantennary	138:148	arg1	N-glycans					150:158	fucosylated biantennary N-glycans	126:158	fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core	126:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	1	35	theme	different	165:173	arg1	N-acetylglucosamine					199:217	N-acetylglucosamine	199:217	N-acetylglucosamine	199:217	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	1	35	theme	different	165:173	arg1	modifications					175:187	different modifications	165:187	different modifications including N-acetylglucosamine, which bisects the mannose core	165:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	0	36	theme	Nanogel	69:75	arg1	Electrophoresis					77:91	Capillary Nanogel Electrophoresis	59:91	Capillary Nanogel Electrophoresis	59:91	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	9	37	theme	α1-6-linked	1659:1669	arg1	branch					1679:1684	the α1-6-linked mannose branch	1655:1684	the α1-6-linked mannose branch	1655:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	4	38	theme	IgG	836:838	arg1	sources					819:825	different sources	809:825	different sources of human IgG	809:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	8	39	theme	sialic	1477:1482	arg1	acid					1484:1487	α2-6-linked sialic acid	1465:1487	α2-6-linked sialic acid	1465:1487	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	2	40	theme	acid	439:442	arg1	levels					410:415	varying levels	402:415	varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine	402:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	1	41	with	N-glycans	150:158	arg1	N-acetylglucosamine					199:217	N-acetylglucosamine	199:217	N-acetylglucosamine	199:217	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	1	41	with	N-glycans	150:158	arg1	modifications					175:187	different modifications	165:187	different modifications including N-acetylglucosamine, which bisects the mannose core	165:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	4	42	theme	different	809:817	arg1	sources					819:825	different sources	809:825	different sources of human IgG	809:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	2	43	theme	α2-6-linked	420:430	arg1	acid					439:442	α2-6-linked sialic acid	420:442	α2-6-linked sialic acid	420:442	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	1	44	theme	Glycosylated	94:105	arg1	IgG					113:115	Glycosylated human IgG	94:115	Glycosylated human IgG	94:115	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	2	45	theme	N-acetylglucosamine	469:487	arg1	levels					410:415	varying levels	402:415	varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine	402:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	8	46	dep	Sambucus	1353:1360	arg1	nigra					1362:1366	nigra	1362:1366	nigra	1362:1366	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	5	47	contain	contain	910:916	arg1	nanogels					891:898	self-assembled nanogels	876:898	self-assembled nanogels that also contain a single stationary zone of lectin	876:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	5	47	contain	contain	910:916	arg2	zone					938:941	a single stationary zone	918:941	a single stationary zone of lectin	918:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	8	48	theme	bisected	1494:1501	arg1	N-acetylglucosamine					1503:1521	bisected N-acetylglucosamine	1494:1521	bisected N-acetylglucosamine	1494:1521	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	0	49	theme	N-Glycan	14:21	arg1	Composition					23:33	the N-Glycan Composition	10:33	the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis	10:91	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	9	50	link	α1-6-linked	1659:1669	arg1	branch					1679:1684	the α1-6-linked mannose branch	1655:1684	the α1-6-linked mannose branch	1655:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	4	51	theme	automated	623:631	arg1	method					633:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	9	52	contain	contain	1623:1629	arg1	N-glycans					1603:1611	bisected N-glycans	1594:1611	bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch	1594:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	9	52	contain	contain	1623:1629	arg2	galactose					1642:1650	a terminal galactose	1631:1650	a terminal galactose on the α1-6-linked mannose branch	1631:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	10	53	theme	lower	1822:1826	arg1	affinity					1828:1835	The lower affinity	1818:1835	The lower affinity to these motifs	1818:1851	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	2	54	theme	galactose	445:453	arg1	levels					410:415	varying levels	402:415	varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine	402:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	4	55	link	N-linked	672:679	arg1	glycans					685:691	N-linked IgG glycans	672:691	N-linked IgG glycans	672:691	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	10	56	theme	ECL	1974:1976	arg1	lectin					1978:1983	the ECL lectin	1970:1983	the ECL lectin	1970:1983	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	12	57	with	compatible	2239:2248	arg1	injections					2287:2296	electrokinetic and hydrodynamic injections	2255:2296	electrokinetic and hydrodynamic injections	2255:2296	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	12	57	with	compatible	2239:2248	arg1	limits					2314:2319	detection limits	2304:2319	detection limits of 70 and 300 pM	2304:2336	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	7	58	theme	bisected	1273:1280	arg1	N-glycan					1282:1289	the monogalactosylated bisected N-glycan	1250:1289	the monogalactosylated bisected N-glycan	1250:1289	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	9	59	theme	terminal	1743:1750	arg1	galactose					1752:1760	terminal galactose	1743:1760	terminal galactose	1743:1760	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	10	60	theme	bisected	2038:2045	arg1	N-glycan					2059:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	3	61	theme	bisecting	527:535	arg1	N-acetylglucosamine					537:555	bisecting N-acetylglucosamine	527:555	bisecting N-acetylglucosamine	527:555	Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiological processes.					
30525457	11	62	theme	peak	2134:2137	arg1	area					2139:2142	peak area	2134:2142	peak area	2134:2142	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	9	63	theme	agalactosylated	1770:1784	arg1	N-glycans					1807:1815	agalactosylated bisected biantennary N-glycans	1770:1815	agalactosylated bisected biantennary N-glycans	1770:1815	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	4	64	theme	IgG	681:683	arg1	glycans					685:691	N-linked IgG glycans	672:691	N-linked IgG glycans	672:691	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	10	65	theme	N-glycan	2059:2066	arg1	composition					2003:2013	the composition	1999:2013	the composition of the agalactosylated bisected biantennary N-glycan	1999:2066	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	9	66	theme	biantennary	1795:1805	arg1	N-glycans					1807:1815	agalactosylated bisected biantennary N-glycans	1770:1815	agalactosylated bisected biantennary N-glycans	1770:1815	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	11	67	dep	%	2180:2180	arg1	deviation					2200:2208	relative standard deviation	2182:2208	less than or equal to 0.08 and 4% relative standard deviation	2148:2208	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	1	68	contain	contains	117:124	arg2	N-glycans					150:158	fucosylated biantennary N-glycans	126:158	fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core	126:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	1	68	contain	contains	117:124	arg1	IgG					113:115	Glycosylated human IgG	94:115	Glycosylated human IgG	94:115	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	10	69	theme	peak	1905:1908	arg1	width					1910:1914	peak width	1905:1914	peak width	1905:1914	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	8	70	theme	Aleuria	1292:1298	arg1	lectin					1309:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin	1292:1314	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	9	71	from	galactose	1642:1650	arg1	branch					1679:1684	the α1-6-linked mannose branch	1655:1684	the α1-6-linked mannose branch	1655:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	11	72	from	precision	2102:2110	arg1	area					2139:2142	peak area	2134:2142	peak area	2134:2142	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	11	72	from	precision	2102:2110	arg1	time					2125:2128	migration time	2115:2128	migration time	2115:2128	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	10	73	dep	authenticates	1985:1997	arg1	change					1895:1900	a change	1893:1900	a change	1893:1900	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	9	74	contain	has	1699:1701	arg2	affinity					1709:1716	lower affinity	1703:1716	lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans	1703:1815	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	9	74	contain	has	1699:1701	arg1	lectin					1692:1697	this lectin	1687:1697	this lectin	1687:1697	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	9	75	theme	binding	1573:1579	arg1	affinity					1581:1588	a strong binding affinity	1564:1588	a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch	1564:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	7	76	theme	positional	1184:1193	arg1	isomers					1195:1201	the positional isomers	1180:1201	the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan	1180:1289	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	5	77	theme	lectin	946:951	arg1	zone					938:941	a single stationary zone	918:941	a single stationary zone of lectin	918:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	8	78	theme	Erythrina	1317:1325	arg1	lectin					1339:1344	Erythrina cristagalli lectin	1317:1344	Erythrina cristagalli lectin (ECL)	1317:1350	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	78	theme	Erythrina	1317:1325	arg1	ECL					1347:1349	ECL	1347:1349	ECL	1347:1349	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	1	79	gly	fucosylated	126:136	arg1	N-glycans					150:158	fucosylated biantennary N-glycans	126:158	fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core	126:249	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	2	80	theme	IgG	330:332	arg1	N-glycans					334:342	human IgG N-glycans	324:342	human IgG N-glycans	324:342	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	8	81	theme	Phaseolus	1380:1388	arg1	PHA-E					1418:1422	PHA-E	1418:1422	PHA-E	1418:1422	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	8	81	theme	Phaseolus	1380:1388	arg1	Erythroagglutinin					1399:1415	Phaseolus vulgaris Erythroagglutinin	1380:1415	Phaseolus vulgaris Erythroagglutinin (PHA-E)	1380:1423	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	7	82	theme	plates	1160:1165	arg1	efficiencies					1136:1147	separation efficiencies	1125:1147	separation efficiencies of 500 000 plates	1125:1165	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	2	83	theme	limited	268:274	arg1	possible					314:321	possible	314:321	possible	314:321	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	2	83	theme	limited	268:274	arg1	number					276:281	only a limited number	261:281	only a limited number of IgG N-glycan structures	261:308	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	5	84	theme	single	920:925	arg1	zone					938:941	a single stationary zone	918:941	a single stationary zone of lectin	918:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	7	85	theme	separation	1125:1134	arg1	efficiencies					1136:1147	separation efficiencies	1125:1147	separation efficiencies of 500 000 plates	1125:1165	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	2	86	theme	N-glycan	290:297	arg1	structures					299:308	IgG N-glycan structures	286:308	IgG N-glycan structures	286:308	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	7	87	theme	nanogel	1091:1097	arg1	electrophoresis					1099:1113	The nanogel electrophoresis	1087:1113	The nanogel electrophoresis	1087:1113	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	0	88	theme	IgG	38:40	arg1	Composition					23:33	the N-Glycan Composition	10:33	the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis	10:91	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	11	89	theme	relative	2182:2189	arg1	deviation					2200:2208	relative standard deviation	2182:2208	less than or equal to 0.08 and 4% relative standard deviation	2148:2208	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	0	90	theme	Capillary	59:67	arg1	Electrophoresis					77:91	Capillary Nanogel Electrophoresis	59:91	Capillary Nanogel Electrophoresis	59:91	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	9	91	theme	mannose	1671:1677	arg1	branch					1679:1684	the α1-6-linked mannose branch	1655:1684	the α1-6-linked mannose branch	1655:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	6	92	theme	N-glycan	1067:1074	arg1	structure					1076:1084	the N-glycan structure	1063:1084	the N-glycan structure	1063:1084	When the lectin specificity matches the N-glycan, the peak disappears from the electropherogram, identifying the N-glycan structure.					
30525457	12	93	theme	pM	2335:2336	arg1	limits					2314:2319	detection limits	2304:2319	detection limits of 70 and 300 pM	2304:2336	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	4	94	theme	human	830:834	arg1	IgG					836:838	human IgG	830:838	human IgG	830:838	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	2	95	contain	contain	394:400	arg2	levels					410:415	varying levels	402:415	varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine	402:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	2	95	contain	contain	394:400	arg1	N-glycans					334:342	human IgG N-glycans	324:342	human IgG N-glycans	324:342	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	9	96	theme	bisected	1594:1601	arg1	N-glycans					1603:1611	bisected N-glycans	1594:1611	bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch	1594:1684	Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans.					
30525457	8	97	theme	α2-6-linked	1465:1475	arg1	acid					1484:1487	α2-6-linked sialic acid	1465:1487	α2-6-linked sialic acid	1465:1487	Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, respectively.					
30525457	2	98	theme	sialic	432:437	arg1	acid					439:442	α2-6-linked sialic acid	420:442	α2-6-linked sialic acid	420:442	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	5	99	theme	self-assembled	876:889	arg1	nanogels					891:898	self-assembled nanogels	876:898	self-assembled nanogels that also contain a single stationary zone of lectin	876:951	The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin.					
30525457	1	100	theme	human	107:111	arg1	IgG					113:115	Glycosylated human IgG	94:115	Glycosylated human IgG	94:115	Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core.					
30525457	2	101	theme	bisected	460:467	arg1	N-acetylglucosamine					469:487	bisected N-acetylglucosamine	460:487	bisected N-acetylglucosamine	460:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	2	102	link	α2-6-linked	420:430	arg1	acid					439:442	α2-6-linked sialic acid	420:442	α2-6-linked sialic acid	420:442	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
30525457	7	103	theme	N-glycan	1282:1289	arg1	isomers					1195:1201	the positional isomers	1180:1201	the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan	1180:1289	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	4	104	theme	rapid	599:603	arg1	method					633:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	0	105	with	Composition	23:33	arg1	Electrophoresis					77:91	Capillary Nanogel Electrophoresis	59:91	Capillary Nanogel Electrophoresis	59:91	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	0	105	with	Composition	23:33	arg1	Lectins					47:53	Lectins	47:53	Lectins	47:53	Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis.					
30525457	3	106	theme	relative	505:512	arg1	abundance					514:522	the relative abundance	501:522	the relative abundance of bisecting N-acetylglucosamine	501:555	Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiological processes.					
30525457	7	107	theme	monogalactosylated	1254:1271	arg1	N-glycan					1282:1289	the monogalactosylated bisected N-glycan	1250:1289	the monogalactosylated bisected N-glycan	1250:1289	The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan.					
30525457	4	108	theme	inexpensive	606:616	arg1	method					633:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method	597:638	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	10	109	theme	agalactosylated	2022:2036	arg1	N-glycan					2059:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	the agalactosylated bisected biantennary N-glycan	2018:2066	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	12	110	theme	detection	2304:2312	arg1	limits					2314:2319	detection limits	2304:2319	detection limits of 70 and 300 pM	2304:2336	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	4	111	theme	N-linked	672:679	arg1	glycans					685:691	N-linked IgG glycans	672:691	N-linked IgG glycans	672:691	A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG.					
30525457	12	112	theme	electrokinetic	2255:2268	arg1	injections					2287:2296	electrokinetic and hydrodynamic injections	2255:2296	electrokinetic and hydrodynamic injections	2255:2296	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	3	113	theme	N-acetylglucosamine	537:555	arg1	abundance					514:522	the relative abundance	501:522	the relative abundance of bisecting N-acetylglucosamine	501:555	Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiological processes.					
30525457	10	114	from	lectin	1978:1983	arg1	results					1957:1963	the results	1953:1963	the results from the ECL lectin	1953:1983	The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan.					
30525457	11	115	dep	0.08	2170:2173	arg1	to					2167:2168	to	2167:2168	to	2167:2168	For runs performed at 17 °C, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, respectively.					
30525457	12	116	theme	hydrodynamic	2274:2285	arg1	injections					2287:2296	electrokinetic and hydrodynamic injections	2255:2296	electrokinetic and hydrodynamic injections	2255:2296	The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, respectively.					
30525457	2	117	theme	varying	402:408	arg1	levels					410:415	varying levels	402:415	varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine	402:487	Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine.					
31759178	7	0	theme	intact	1879:1884	arg1	engine					1908:1913	intact N-glycopeptide search engine	1879:1913	intact N-glycopeptide search engine GPSeeker	1879:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	8	1	theme	spectrum-level	1930:1943	arg1	%					1952:1952	spectrum-level FDR ≤ 1%	1930:1952	spectrum-level FDR ≤ 1%	1930:1952	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	3	2	theme	quantitative	637:648	arg1	study					668:672	our site- and structure-specific relatively quantitative N-glycoproteomics study	593:672	our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	593:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	4	3	theme	N-glycan	955:962	arg1	moieties					964:971	the N-glycan moieties	951:971	the N-glycan moieties (monosaccharide composition, sequence and linkages)	951:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	3	theme	N-glycan	955:962	arg1	composition					989:999	monosaccharide composition	974:999	monosaccharide composition	974:999	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	3	theme	N-glycan	955:962	arg1	linkages					1015:1022	linkages	1015:1022	linkages	1015:1022	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	3	theme	N-glycan	955:962	arg1	sequence					1002:1009	sequence	1002:1009	sequence	1002:1009	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	7	4	theme	stepped	1861:1867	arg1	NCEs					1869:1872	stepped NCEs	1861:1872	stepped NCEs	1861:1872	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	2	5	gly	N-glycopeptide	298:311	arg2	N-glycopeptide					298:311	intact N-glycopeptide search engine	291:325	intact N-glycopeptide search engine GPSeeker	291:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	2	6	theme	intact	291:296	arg1	engine					320:325	intact N-glycopeptide search engine	291:325	intact N-glycopeptide search engine GPSeeker	291:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	3	7	theme	breast	677:682	arg1	cells					697:701	breast MCF-7 cancer cells	677:701	breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	677:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	6	8	dep	14	1401:1402	arg1	each					1405:1408	each	1405:1408	each	1405:1408	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	7	9	gly	N-glycopeptide	1886:1899	arg2	N-glycopeptide					1886:1899	intact N-glycopeptide search engine	1879:1913	intact N-glycopeptide search engine GPSeeker	1879:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	6	10	dep	observed	1568:1575	arg1	whereas					1552:1558	whereas	1552:1558	whereas	1552:1558	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	9	11	theme	putative	2238:2245	arg1	structures					2255:2264	the 114 N-glycan putative linkage structures	2221:2264	the 114 N-glycan putative linkage structures	2221:2264	For the 114 N-glycan putative linkage structures, 44 were confirmed with no less than one structure-diagnostic fragment ions.					
31759178	8	12	theme	comprehensive	1987:1999	arg1	information					2012:2022	comprehensive structural information	1987:2022	comprehensive structural information of both the peptide backbones and the N-glycan moieties	1987:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	5	13	theme	thrice	1143:1148	arg1	p ≤ .05					1213:1219	p ≤ .05	1213:1219	p ≤ .05	1213:1219	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	5	13	theme	thrice	1143:1148	arg1	%					1233:1233	RSD ≤ 20%	1225:1233	RSD ≤ 20%	1225:1233	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	5	13	theme	thrice	1143:1148	arg1	criteria					1111:1118	the criteria	1107:1118	the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change	1107:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	2	14	theme	GPSeeker	327:334	arg1	development					276:286	Our recent development	265:286	Our recent development of intact N-glycopeptide search engine GPSeeker	265:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	7	15	theme	cancer	1670:1675	arg1	cells					1677:1681	breast MCF-7 cancer cells	1657:1681	breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1657:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	1	16	theme	pathological	222:233	arg1	cancers					256:262	cancers	256:262	cancers	256:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	16	theme	pathological	222:233	arg1	conditions					235:244	various pathological conditions	214:244	various pathological conditions including cancers	214:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	7	17	theme	breast	1657:1662	arg1	cells					1677:1681	breast MCF-7 cancer cells	1657:1681	breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1657:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	0	18	from	N-glycosylation	82:96	arg1	cells					114:118	MCF-7 cancer cells	101:118	MCF-7 cancer cells	101:118	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	4	19	theme	intact	815:820	arg1	N-glycopeptides					822:836	581 intact N-glycopeptides	811:836	581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	811:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	3	20	theme	cancer	690:695	arg1	cells					697:701	breast MCF-7 cancer cells	677:701	breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	677:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	1	21	theme	common	140:145	arg1	PTM					155:157	a common protein PTM	138:157	a common protein PTM	138:157	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	21	theme	common	140:145	arg1	Glycosylation					121:133	Glycosylation	121:133	Glycosylation	121:133	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	7	22	theme	structure-specific	1789:1806	arg1	N-glycoproteomics					1808:1824	site- and structure-specific N-glycoproteomics	1779:1824	site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker	1779:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	6	23	theme	N-glycosylation	1373:1387	arg1	expression					1389:1398	differential N-glycosylation expression	1360:1398	differential N-glycosylation expression	1360:1398	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	5	24	theme	at	1134:1135	arg1	thrice					1143:1148	quantified at least thrice	1123:1148	quantified at least thrice out of the five technical replicates with no <1.5-fold change	1123:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	3	25	theme	MCF-10A	727:733	arg1	cells					735:739	epithelial MCF-10A cells	716:739	epithelial MCF-10A cells	716:739	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	6	26	theme	down	1594:1597	arg1	regulation					1599:1608	down regulation	1594:1608	down regulation	1594:1608	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	7	27	theme	site-	1779:1783	arg1	N-glycoproteomics					1808:1824	site- and structure-specific N-glycoproteomics	1779:1824	site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker	1779:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	10	28	theme	1.5-fold	2438:2445	arg1	change					2447:2452	no < 1.5-fold change	2433:2452	no < 1.5-fold change	2433:2452	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	5	29	with	technical	1166:1174	arg1	change					1205:1210	no <1.5-fold change	1192:1210	no <1.5-fold change	1192:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	8	30	theme	putative	2109:2116	arg1	N-glycosites					2118:2129	the 248 putative N-glycosites	2101:2129	the 248 putative N-glycosites	2101:2129	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	2	31	theme	moieties	568:575	arg1	ions					547:550	structure-specific fragment ions	519:550	structure-specific fragment ions	519:550	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	2	31	theme	moieties	568:575	arg1	backbones					505:513	peptide backbones	497:513	peptide backbones	497:513	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	8	32	theme	peptide	2036:2042	arg1	backbones					2044:2052	the peptide backbones	2032:2052	the peptide backbones	2032:2052	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	0	33	theme	MCF-7	101:105	arg1	cells					114:118	MCF-7 cancer cells	101:118	MCF-7 cancer cells	101:118	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	7	34	dep	cells	1677:1681	arg1	relative					1684:1691	relative	1684:1691	relative	1684:1691	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	7	35	from	N-glycosylation	1638:1652	arg1	cells					1677:1681	breast MCF-7 cancer cells	1657:1681	breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1657:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	1	36	theme	aberrant	168:175	arg1	regulation					177:186	its aberrant regulation	164:186	its aberrant regulation	164:186	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	9	37	theme	structure-diagnostic	2307:2326	arg1	ions					2337:2340	one structure-diagnostic fragment ions	2303:2340	one structure-diagnostic fragment ions	2303:2340	For the 114 N-glycan putative linkage structures, 44 were confirmed with no less than one structure-diagnostic fragment ions.					
31759178	2	38	theme	search	313:318	arg1	engine					320:325	intact N-glycopeptide search engine	291:325	intact N-glycopeptide search engine GPSeeker	291:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	5	39	gly	N-glycoproteins	1295:1309	arg1	N-glycoproteins					1295:1309	19 intact N-glycoproteins	1285:1309	19 intact N-glycoproteins	1285:1309	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	4	40	theme	backbones	900:908	arg1	information					868:878	comprehensive structural information	843:878	comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	843:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	2	41	theme	structure-specific	519:536	arg1	ions					547:550	structure-specific fragment ions	519:550	structure-specific fragment ions	519:550	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	4	42	theme	structural	857:866	arg1	information					868:878	comprehensive structural information	843:878	comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	843:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	2	43	theme	DEGPs	460:464	arg1	characterization					391:406	relatively quantitative structure-specific characterization	348:406	relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs)	348:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	4	44	dep	moieties	964:971	arg1	moieties					964:971	the N-glycan moieties	951:971	the N-glycan moieties (monosaccharide composition, sequence and linkages)	951:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	44	dep	moieties	964:971	arg1	composition					989:999	monosaccharide composition	974:999	monosaccharide composition	974:999	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	44	dep	moieties	964:971	arg1	linkages					1015:1022	linkages	1015:1022	linkages	1015:1022	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	44	dep	moieties	964:971	arg1	sequence					1002:1009	sequence	1002:1009	sequence	1002:1009	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	8	45	theme	intact	1959:1964	arg1	N-glycopeptides					1966:1980	581 intact N-glycopeptides	1955:1980	581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties	1955:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	7	46	theme	MCF-10A	1696:1702	arg1	cells					1715:1719	MCF-10A epithelial cells	1696:1719	MCF-10A epithelial cells	1696:1719	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	2	47	theme	backbones	505:513	arg1	labeling					481:488	isotopic labeling	472:488	isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties	472:575	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	2	48	theme	intact	436:441	arg1	DEGPs					460:464	differentially expressed intact N-glycopeptides (DEGPs)	411:465	differentially expressed intact N-glycopeptides (DEGPs)	411:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	5	49	gly	23 N-glycosites	1266:1280	arg2	23 N-glycosites					1266:1280	23 N-glycosites	1266:1280	23 N-glycosites on 19 intact N-glycoproteins	1266:1309	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	8	50	with	N-glycopeptides	1966:1980	arg1	information					2012:2022	comprehensive structural information	1987:2022	comprehensive structural information of both the peptide backbones and the N-glycan moieties	1987:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	0	51	theme	structure-specific	10:27	arg1	N-glycoproteomics					42:58	structure-specific quantitative N-glycoproteomics	10:58	structure-specific quantitative N-glycoproteomics	10:58	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	7	52	with	HCD	1852:1854	arg1	NCEs					1869:1872	stepped NCEs	1861:1872	stepped NCEs	1861:1872	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	2	53	theme	ions	547:550	arg1	labeling					481:488	isotopic labeling	472:488	isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties	472:575	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	6	54	theme	down	1461:1464	arg1	regulation					1466:1475	uniform down regulation	1453:1475	uniform down regulation	1453:1475	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	7	55	gly	N-glycosylation	1638:1652	arg1	cells					1677:1681	breast MCF-7 cancer cells	1657:1681	breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1657:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	5	56	dep	technical	1166:1174	arg1	replicates					1176:1185	replicates	1176:1185	replicates	1176:1185	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	1	57	attach	linked	204:209	arg1	cancers					256:262	cancers	256:262	cancers	256:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	57	attach	linked	204:209	arg2	regulation					177:186	its aberrant regulation	164:186	its aberrant regulation	164:186	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	57	attach	linked	204:209	arg1	conditions					235:244	various pathological conditions	214:244	various pathological conditions including cancers	214:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	4	58	theme	amino	911:915	arg1	N-glycosites					933:944	N-glycosites	933:944	N-glycosites	933:944	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	58	theme	amino	911:915	arg1	sequences					922:930	amino acid sequences	911:930	amino acid sequences	911:930	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	2	59	theme	structure-specific	372:389	arg1	characterization					391:406	relatively quantitative structure-specific characterization	348:406	relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs)	348:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	3	60	theme	structure-specific	607:624	arg1	study					668:672	our site- and structure-specific relatively quantitative N-glycoproteomics study	593:672	our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	593:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	7	61	theme	N-glycopeptide	1886:1899	arg1	engine					1908:1913	intact N-glycopeptide search engine	1879:1913	intact N-glycopeptide search engine GPSeeker	1879:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	10	62	dep	observed	2654:2661	arg1	whereas					2637:2643	whereas	2637:2643	whereas	2637:2643	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	2	63	theme	N-glycopeptides	443:457	arg1	DEGPs					460:464	differentially expressed intact N-glycopeptides (DEGPs)	411:465	differentially expressed intact N-glycopeptides (DEGPs)	411:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	4	64	with	N-glycopeptides	822:836	arg1	information					868:878	comprehensive structural information	843:878	comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	843:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	6	65	theme	differential	1360:1371	arg1	expression					1389:1398	differential N-glycosylation expression	1360:1398	differential N-glycosylation expression	1360:1398	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	2	66	theme	expressed	426:434	arg1	DEGPs					460:464	differentially expressed intact N-glycopeptides (DEGPs)	411:465	differentially expressed intact N-glycopeptides (DEGPs)	411:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	10	67	dep	technical	2407:2415	arg1	replicates					2417:2426	replicates	2417:2426	replicates	2417:2426	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	4	68	theme	monosaccharide	974:987	arg1	moieties					964:971	the N-glycan moieties	951:971	the N-glycan moieties (monosaccharide composition, sequence and linkages)	951:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	68	theme	monosaccharide	974:987	arg1	composition					989:999	monosaccharide composition	974:999	monosaccharide composition	974:999	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	8	69	gly	N-glycopeptides	1966:1980	arg2	N-glycopeptides					1966:1980	581 intact N-glycopeptides	1955:1980	581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties	1955:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	10	70	gly	N-glycoproteins	2507:2521	arg1	N-glycoproteins					2507:2521	21 intact N-glycoproteins	2497:2521	21 intact N-glycoproteins	2497:2521	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	2	71	theme	engine	320:325	arg1	GPSeeker					327:334	intact N-glycopeptide search engine GPSeeker	291:334	intact N-glycopeptide search engine GPSeeker	291:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	10	72	with	technical	2407:2415	arg1	p ≤ .05					2458:2464	p ≤ .05	2458:2464	p ≤ .05	2458:2464	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	10	72	with	technical	2407:2415	arg1	change					2447:2452	no < 1.5-fold change	2433:2452	no < 1.5-fold change	2433:2452	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	6	73	theme	intact	1323:1328	arg1	N-glycoproteins					1330:1344	the 19 intact N-glycoproteins	1316:1344	the 19 intact N-glycoproteins observed with differential N-glycosylation expression	1316:1398	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	2	74	theme	N-glycopeptide	298:311	arg1	engine					320:325	intact N-glycopeptide search engine	291:325	intact N-glycopeptide search engine GPSeeker	291:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	4	75	gly	N-glycosites	933:944	arg2	N-glycosites					933:944	N-glycosites	933:944	N-glycosites	933:944	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	75	gly	N-glycosites	933:944	arg2	sequences					922:930	amino acid sequences	911:930	amino acid sequences	911:930	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	8	76	theme	N-glycan	2062:2069	arg1	moieties					2071:2078	the N-glycan moieties	2058:2078	the N-glycan moieties	2058:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	3	77	dep	cells	697:701	arg1	relative					704:711	relative	704:711	relative	704:711	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	10	78	dep	criteria	2352:2359	arg1	quantified					2364:2373	quantified	2364:2373	quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05	2364:2464	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	4	79	theme	moieties	964:971	arg1	information					868:878	comprehensive structural information	843:878	comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	843:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	9	80	theme	114 N-glycan	2225:2236	arg1	structures					2255:2264	the 114 N-glycan putative linkage structures	2221:2264	the 114 N-glycan putative linkage structures	2221:2264	For the 114 N-glycan putative linkage structures, 44 were confirmed with no less than one structure-diagnostic fragment ions.					
31759178	5	81	from	23 N-glycosites	1266:1280	arg1	N-glycoproteins					1295:1309	19 intact N-glycoproteins	1285:1309	19 intact N-glycoproteins	1285:1309	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	3	82	theme	N-glycoproteomics	650:666	arg1	study					668:672	our site- and structure-specific relatively quantitative N-glycoproteomics study	593:672	our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	593:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	7	83	theme	MCF-7	1664:1668	arg1	cells					1677:1681	breast MCF-7 cancer cells	1657:1681	breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1657:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	7	84	theme	engine	1908:1913	arg1	GPSeeker					1915:1922	intact N-glycopeptide search engine GPSeeker	1879:1922	intact N-glycopeptide search engine GPSeeker	1879:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	9	85	theme	linkage	2247:2253	arg1	structures					2255:2264	the 114 N-glycan putative linkage structures	2221:2264	the 114 N-glycan putative linkage structures	2221:2264	For the 114 N-glycan putative linkage structures, 44 were confirmed with no less than one structure-diagnostic fragment ions.					
31759178	4	86	gly	N-glycopeptides	822:836	arg2	N-glycopeptides					822:836	581 intact N-glycopeptides	811:836	581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	811:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	3	87	theme	cells	697:701	arg1	study					668:672	our site- and structure-specific relatively quantitative N-glycoproteomics study	593:672	our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	593:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	6	88	theme	up	1537:1538	arg1	regulation					1540:1549	uniform up regulation	1529:1549	uniform up regulation	1529:1549	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	1	89	theme	various	214:220	arg1	cancers					256:262	cancers	256:262	cancers	256:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	89	theme	various	214:220	arg1	conditions					235:244	various pathological conditions	214:244	various pathological conditions including cancers	214:262	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	10	90	theme	least	2378:2382	arg1	thrice					2384:2389	least thrice	2378:2389	least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05	2378:2464	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	10	91	theme	intact	2533:2538	arg1	N-glycoproteins					2540:2554	13 and 5 intact N-glycoproteins	2524:2554	13 and 5 intact N-glycoproteins (each with one or more DEGPs)	2524:2584	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	3	92	theme	MCF-7	684:688	arg1	cells					697:701	breast MCF-7 cancer cells	677:701	breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	677:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	7	93	theme	Differential	1625:1636	arg1	N-glycosylation					1638:1652	Differential N-glycosylation	1625:1652	Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells)	1625:1720	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	8	94	theme	structural	2001:2010	arg1	information					2012:2022	comprehensive structural information	1987:2022	comprehensive structural information of both the peptide backbones and the N-glycan moieties	1987:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	8	95	theme	backbones	2044:2052	arg1	information					2012:2022	comprehensive structural information	1987:2022	comprehensive structural information of both the peptide backbones and the N-glycan moieties	1987:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	5	96	theme	quantified	1123:1132	arg1	thrice					1143:1148	quantified at least thrice	1123:1148	quantified at least thrice out of the five technical replicates with no <1.5-fold change	1123:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	3	97	theme	epithelial	716:725	arg1	cells					735:739	epithelial MCF-10A cells	716:739	epithelial MCF-10A cells	716:739	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31759178	1	98	theme	protein	147:153	arg1	PTM					155:157	a common protein PTM	138:157	a common protein PTM	138:157	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	1	98	theme	protein	147:153	arg1	Glycosylation					121:133	Glycosylation	121:133	Glycosylation	121:133	Glycosylation is a common protein PTM, and its aberrant regulation has been widely linked to various pathological conditions including cancers.					
31759178	6	99	theme	uniform	1453:1459	arg1	regulation					1466:1475	uniform down regulation	1453:1475	uniform down regulation	1453:1475	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	5	100	theme	least	1137:1141	arg1	thrice					1143:1148	quantified at least thrice	1123:1148	quantified at least thrice out of the five technical replicates with no <1.5-fold change	1123:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	2	101	theme	recent	269:274	arg1	development					276:286	Our recent development	265:286	Our recent development of intact N-glycopeptide search engine GPSeeker	265:334	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	4	102	dep	backbones	900:908	arg1	N-glycosites					933:944	N-glycosites	933:944	N-glycosites	933:944	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	102	dep	backbones	900:908	arg1	sequences					922:930	amino acid sequences	911:930	amino acid sequences	911:930	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	10	103	theme	<	2436:2436	arg1	change					2447:2452	no < 1.5-fold change	2433:2452	no < 1.5-fold change	2433:2452	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	10	104	theme	uniform	2605:2611	arg1	down					2613:2616	uniform down	2605:2616	uniform down	2605:2616	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	4	105	theme	spectrum-level	786:799	arg1	%					808:808	spectrum-level FDR ≤ 1%	786:808	spectrum-level FDR ≤ 1%	786:808	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	0	106	theme	cancer	107:112	arg1	cells					114:118	MCF-7 cancer cells	101:118	MCF-7 cancer cells	101:118	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	5	107	theme	<1.5-fold	1195:1203	arg1	change					1205:1210	no <1.5-fold change	1192:1210	no <1.5-fold change	1192:1210	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	6	108	gly	N-glycoproteins	1330:1344	arg1	N-glycoproteins					1330:1344	the 19 intact N-glycoproteins	1316:1344	the 19 intact N-glycoproteins observed with differential N-glycosylation expression	1316:1398	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	2	109	theme	N-glycan	559:566	arg1	moieties					568:575	the N-glycan moieties	555:575	the N-glycan moieties	555:575	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	10	110	theme	down	2688:2691	arg1	regulation					2693:2702	down regulation	2688:2702	down regulation	2688:2702	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	4	111	dep	technical	1051:1059	arg1	TR5					1096:1098	TR5	1096:1098	TR5	1096:1098	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	111	dep	technical	1051:1059	arg1	TR2					1078:1080	TR2	1078:1080	TR2	1078:1080	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	111	dep	technical	1051:1059	arg1	TR3					1083:1085	TR3	1083:1085	TR3	1083:1085	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	111	dep	technical	1051:1059	arg1	replicates					1061:1070	replicates	1061:1070	replicates	1061:1070	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	111	dep	technical	1051:1059	arg1	TR1					1073:1075	TR1	1073:1075	TR1	1073:1075	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	111	dep	technical	1051:1059	arg1	TR4					1088:1090	TR4	1088:1090	TR4	1088:1090	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	0	112	gly	N-glycosylation	82:96	arg1	cells					114:118	MCF-7 cancer cells	101:118	MCF-7 cancer cells	101:118	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	9	113	theme	fragment	2328:2335	arg1	ions					2337:2340	one structure-diagnostic fragment ions	2303:2340	one structure-diagnostic fragment ions	2303:2340	For the 114 N-glycan putative linkage structures, 44 were confirmed with no less than one structure-diagnostic fragment ions.					
31759178	2	114	dep	backbones	505:513	arg1	the					493:495	the	493:495	the	493:495	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	8	115	dep	as	2196:2197	arg1	of					2199:2200	of	2199:2200	of	2199:2200	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	4	116	theme	peptide	892:898	arg1	backbones					900:908	the peptide backbones	888:908	the peptide backbones (amino acid sequences, N-glycosites)	888:945	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	10	117	dep	N-glycoproteins	2540:2554	arg1	each					2557:2560	each	2557:2560	each	2557:2560	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	4	118	theme	comprehensive	843:855	arg1	information					868:878	comprehensive structural information	843:878	comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages)	843:1023	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	0	119	theme	quantitative	29:40	arg1	N-glycoproteomics					42:58	structure-specific quantitative N-glycoproteomics	10:58	structure-specific quantitative N-glycoproteomics	10:58	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	8	120	gly	N-glycosites	2118:2129	arg2	N-glycosites					2118:2129	the 248 putative N-glycosites	2101:2129	the 248 putative N-glycosites	2101:2129	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	7	121	theme	epithelial	1704:1713	arg1	cells					1715:1719	MCF-10A epithelial cells	1696:1719	MCF-10A epithelial cells	1696:1719	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	2	122	theme	peptide	497:503	arg1	backbones					505:513	peptide backbones	497:513	peptide backbones	497:513	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	2	123	gly	N-glycopeptides	443:457	arg2	N-glycopeptides					443:457	differentially expressed intact N-glycopeptides (DEGPs)	411:465	differentially expressed intact N-glycopeptides (DEGPs)	411:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	10	124	theme	up	2622:2623	arg1	regulation					2625:2634	up regulation	2622:2634	up regulation	2622:2634	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	6	125	dep	6	1478:1478	arg1	each					1481:1484	each	1481:1484	each	1481:1484	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	10	126	theme	intact	2500:2505	arg1	N-glycoproteins					2507:2521	21 intact N-glycoproteins	2497:2521	21 intact N-glycoproteins	2497:2521	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	8	127	theme	moieties	2071:2078	arg1	information					2012:2022	comprehensive structural information	1987:2022	comprehensive structural information of both the peptide backbones and the N-glycan moieties	1987:2078	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones and the N-glycan moieties were identified; For the 248 putative N-glycosites, 248 were confirmed where 125 have not been annotated in UniProt as of July 25, 2019.					
31759178	2	128	theme	isotopic	472:479	arg1	labeling					481:488	isotopic labeling	472:488	isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties	472:575	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	0	129	theme	differential	69:80	arg1	N-glycosylation					82:96	differential N-glycosylation	69:96	differential N-glycosylation in MCF-7 cancer cells	69:118	Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.					
31759178	10	130	gly	N-glycoproteins	2540:2554	arg1	N-glycoproteins					2540:2554	13 and 5 intact N-glycoproteins	2524:2554	13 and 5 intact N-glycoproteins (each with one or more DEGPs)	2524:2584	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	2	131	theme	fragment	538:545	arg1	ions					547:550	structure-specific fragment ions	519:550	structure-specific fragment ions	519:550	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	6	132	theme	uniform	1529:1535	arg1	regulation					1540:1549	uniform up regulation	1529:1549	uniform up regulation	1529:1549	For the 19 intact N-glycoproteins observed with differential N-glycosylation expression, 14 (each with one or more DEGPs) were observed with uniform down regulation; 6 (each with one or more DEGPs) were observed with uniform up regulation; whereas one was observed with both up and down regulation.					
31759178	10	133	theme	simultaneous	2668:2679	arg1	up					2681:2682	simultaneous up	2668:2682	simultaneous up	2668:2682	With the criteria of quantified at least thrice out of the five technical replicates with no < 1.5-fold change and p ≤ .05, 56 DEGPs were quantified from 21 intact N-glycoproteins; 13 and 5 intact N-glycoproteins (each with one or more DEGPs) were observed with uniform down and up regulation; whereas one were observed with simultaneous up and down regulation.					
31759178	2	134	theme	quantitative	359:370	arg1	characterization					391:406	relatively quantitative structure-specific characterization	348:406	relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs)	348:465	Our recent development of intact N-glycopeptide search engine GPSeeker has enabled relatively quantitative structure-specific characterization of differentially expressed intact N-glycopeptides (DEGPs) with isotopic labeling of the peptide backbones and structure-specific fragment ions of the N-glycan moieties.					
31759178	5	135	theme	intact	1288:1293	arg1	N-glycoproteins					1295:1309	19 intact N-glycoproteins	1285:1309	19 intact N-glycoproteins	1285:1309	With the criteria of quantified at least thrice out of the five technical replicates with no <1.5-fold change, p ≤ .05 and RSD ≤ 20%, 56 DEGPs were quantified from 23 N-glycosites on 19 intact N-glycoproteins.					
31759178	7	136	theme	search	1901:1906	arg1	engine					1908:1913	intact N-glycopeptide search engine	1879:1913	intact N-glycopeptide search engine GPSeeker	1879:1922	SIGNIFICANCE: Differential N-glycosylation in breast MCF-7 cancer cells (relative to MCF-10A epithelial cells) were qualitatively and quantitatively characterized with site- and structure-specific N-glycoproteomics using RPLC-nanoESI-MS/MS (HCD with stepped NCEs) and intact N-glycopeptide search engine GPSeeker.					
31759178	4	137	theme	acid	917:920	arg1	N-glycosites					933:944	N-glycosites	933:944	N-glycosites	933:944	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	4	137	theme	acid	917:920	arg1	sequences					922:930	amino acid sequences	911:930	amino acid sequences	911:930	With spectrum-level FDR ≤ 1%, 581 intact N-glycopeptides with comprehensive structural information of both the peptide backbones (amino acid sequences, N-glycosites) and the N-glycan moieties (monosaccharide composition, sequence and linkages) were identified from five technical replicates (TR1, TR2, TR3, TR4 and TR5).					
31759178	3	138	theme	site-	597:601	arg1	study					668:672	our site- and structure-specific relatively quantitative N-glycoproteomics study	593:672	our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells)	593:740	Here we report our site- and structure-specific relatively quantitative N-glycoproteomics study of breast MCF-7 cancer cells (relative to epithelial MCF-10A cells) using RPLC-nanoESI-MS/MS and GPSeeker.					
31279935	0	0	theme	cellulase	75:83	arg1	dynamics					43:50	dynamics	43:50	dynamics	43:50	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	0	0	theme	cellulase	75:83	arg1	structure					29:37	structure	29:37	structure	29:37	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	10	1	theme	reduced	1097:1103	arg1	flexibility					1105:1115	the reduced flexibility	1093:1115	the reduced flexibility	1093:1115	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
31279935	1	2	theme	bioethanol	156:165	arg1	production					167:176	bioethanol production	156:176	bioethanol production	156:176	Fungi cellulases are used to degrade cellulose-containing biomass for bioethanol production.					
31279935	8	3	theme	Essential	834:842	arg1	analysis					853:860	Essential dynamics analysis	834:860	Essential dynamics analysis	834:860	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	0	4	theme	Cel7A	69:73	arg1	cellulase					75:83	a full-length Cel7A cellulase	55:83	a full-length Cel7A cellulase	55:83	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	0	5	from	effects	14:20	arg1	dynamics					43:50	dynamics	43:50	dynamics	43:50	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	0	5	from	effects	14:20	arg1	structure					29:37	structure	29:37	structure	29:37	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	7	6	from	effects	770:776	arg1	dynamics					799:806	dynamics	799:806	dynamics	799:806	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	7	6	from	effects	770:776	arg1	structure					785:793	structure	785:793	structure	785:793	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	8	7	from	O-glycosylation	874:888	arg1	linker					897:902	the linker	893:902	the linker	893:902	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	9	8	link	O-linked	958:965	arg1	glycans					967:973	O-linked glycans	958:973	O-linked glycans	958:973	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	12	9	theme	point	1417:1421	arg1	modification					1443:1454	modification	1443:1454	modification of glycosylation sites	1443:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	9	theme	point	1417:1421	arg1	mutations					1423:1431	point mutations	1417:1431	point mutations including modification of glycosylation sites	1417:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	10	theme	design	1500:1505	arg1	flexibility					1402:1412	targeting Cel7A linker flexibility	1379:1412	targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites	1379:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	10	theme	design	1500:1505	arg1	strategy					1507:1514	a promising design strategy	1488:1514	a promising design strategy to improve cellulase activity	1488:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	9	11	theme	O-linked	958:965	arg1	glycans					967:973	O-linked glycans	958:973	O-linked glycans	958:973	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	4	12	theme	glycosylated	476:487	arg1	linker					489:494	a flexible and highly glycosylated linker	454:494	a flexible and highly glycosylated linker	454:494	This cellulase consists of two domains connected by a flexible and highly glycosylated linker.					
31279935	11	13	theme	collapsing	1338:1347	arg1	domains					1319:1325	the functional domains	1304:1325	the functional domains frequently collapsing	1304:1347	In contrast, in the absence of glycosylation, we observed vast conformational plasticity with the functional domains frequently collapsing.					
31279935	9	14	theme	elongated	1060:1068	arg1	conformations					1070:1082	more elongated conformations	1055:1082	more elongated conformations	1055:1082	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	12	15	theme	glycosylation	1459:1471	arg1	sites					1473:1477	glycosylation sites	1459:1477	glycosylation sites	1459:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	2	16	from	reesei	232:237	arg1	cellulases					190:199	Industrial cellulases	179:199	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A)	179:247	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	2	16	from	reesei	232:237	arg1	Cel7A					209:213	Cel7A	209:213	Cel7A	209:213	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	10	17	theme	functional	1118:1127	arg1	motions					1141:1147	functional interdomain motions	1118:1147	functional interdomain motions	1118:1147	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
31279935	5	18	theme	linker	510:515	arg1	flexibility					517:527	the linker flexibility	506:527	the linker flexibility	506:527	However, the linker flexibility has hindered the determination of Cel7A complete structure.					
31279935	6	19	theme	sparse	617:622	arg1	data					624:627	atomic and sparse data	606:627	atomic and sparse data	606:627	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	3	20	theme	dynamics	320:327	arg1	understanding					289:301	the understanding	285:301	the understanding of structure and dynamics	285:327	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	3	20	theme	dynamics	320:327	arg1	crucial					332:338	crucial	332:338	crucial	332:338	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	6	21	theme	complete	687:694	arg1	structure					703:711	the complete enzyme structure	683:711	the complete enzyme structure	683:711	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	12	22	theme	cellulase	1527:1535	arg1	activity					1537:1544	cellulase activity	1527:1544	cellulase activity	1527:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	23	gly	glycosylation	1459:1471	arg2	sites					1473:1477	glycosylation sites	1459:1477	glycosylation sites	1459:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	24	theme	promising	1490:1498	arg1	flexibility					1402:1412	targeting Cel7A linker flexibility	1379:1412	targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites	1379:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	24	theme	promising	1490:1498	arg1	strategy					1507:1514	a promising design strategy	1488:1514	a promising design strategy to improve cellulase activity	1488:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	1	25	theme	Fungi	86:90	arg1	cellulases					92:101	Fungi cellulases	86:101	Fungi cellulases	86:101	Fungi cellulases are used to degrade cellulose-containing biomass for bioethanol production.					
31279935	4	26	gly	glycosylated	476:487	arg1	linker					489:494	a flexible and highly glycosylated linker	454:494	a flexible and highly glycosylated linker	454:494	This cellulase consists of two domains connected by a flexible and highly glycosylated linker.					
31279935	11	27	theme	functional	1308:1317	arg1	domains					1319:1325	the functional domains	1304:1325	the functional domains frequently collapsing	1304:1347	In contrast, in the absence of glycosylation, we observed vast conformational plasticity with the functional domains frequently collapsing.					
31279935	3	28	theme	improved	370:377	arg1	activity					392:399	improved cellulolytic activity	370:399	improved cellulolytic activity	370:399	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	11	29	theme	glycosylation	1241:1253	arg1	absence					1230:1236	the absence	1226:1236	the absence of glycosylation	1226:1253	In contrast, in the absence of glycosylation, we observed vast conformational plasticity with the functional domains frequently collapsing.					
31279935	7	30	theme	TrCel7A	825:831	arg1	dynamics					799:806	dynamics	799:806	dynamics	799:806	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	7	30	theme	TrCel7A	825:831	arg1	structure					785:793	structure	785:793	structure	785:793	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	12	31	theme	targeting	1379:1387	arg1	flexibility					1402:1412	targeting Cel7A linker flexibility	1379:1412	targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites	1379:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	31	theme	targeting	1379:1387	arg1	strategy					1507:1514	a promising design strategy	1488:1514	a promising design strategy to improve cellulase activity	1488:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	32	theme	sites	1473:1477	arg1	modification					1443:1454	modification	1443:1454	modification of glycosylation sites	1443:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	3	33	theme	cellulolytic	379:390	arg1	activity					392:399	improved cellulolytic activity	370:399	improved cellulolytic activity	370:399	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	8	34	gly	O-glycosylation	874:888	arg1	linker					897:902	the linker	893:902	the linker	893:902	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	4	35	theme	flexible	456:463	arg1	linker					489:494	a flexible and highly glycosylated linker	454:494	a flexible and highly glycosylated linker	454:494	This cellulase consists of two domains connected by a flexible and highly glycosylated linker.					
31279935	1	36	used	used	107:110	arg2	cellulases					92:101	Fungi cellulases	86:101	Fungi cellulases	86:101	Fungi cellulases are used to degrade cellulose-containing biomass for bioethanol production.					
31279935	2	37	from	critical	253:260	arg1	process					270:276	this process	265:276	this process	265:276	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	9	38	theme	dihedral	1000:1007	arg1	angles					1009:1014	protein dihedral angles	992:1014	protein dihedral angles distribution	992:1027	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	6	39	theme	structure	703:711	arg1	model					674:678	a model	672:678	a model of the complete enzyme structure	672:711	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	11	40	theme	conformational	1273:1286	arg1	plasticity					1288:1297	vast conformational plasticity	1268:1297	vast conformational plasticity	1268:1297	In contrast, in the absence of glycosylation, we observed vast conformational plasticity with the functional domains frequently collapsing.					
31279935	7	41	theme	solubilized	813:823	arg1	TrCel7A					825:831	a solubilized TrCel7A	811:831	a solubilized TrCel7A	811:831	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	12	42	theme	linker	1395:1400	arg1	flexibility					1402:1412	targeting Cel7A linker flexibility	1379:1412	targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites	1379:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	42	theme	linker	1395:1400	arg1	strategy					1507:1514	a promising design strategy	1488:1514	a promising design strategy to improve cellulase activity	1488:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	9	43	theme	angles	1009:1014	arg1	distribution					1016:1027	protein dihedral angles distribution	992:1027	protein dihedral angles distribution	992:1027	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	2	44	theme	Industrial	179:188	arg1	cellulases					190:199	Industrial cellulases	179:199	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A)	179:247	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	2	44	theme	Industrial	179:188	arg1	Cel7A					209:213	Cel7A	209:213	Cel7A	209:213	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	6	45	theme	atomic	606:611	arg1	data					624:627	atomic and sparse data	606:627	atomic and sparse data	606:627	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	12	46	theme	Cel7A	1389:1393	arg1	flexibility					1402:1412	targeting Cel7A linker flexibility	1379:1412	targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites	1379:1477	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	12	46	theme	Cel7A	1389:1393	arg1	strategy					1507:1514	a promising design strategy	1488:1514	a promising design strategy to improve cellulase activity	1488:1544	We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.					
31279935	11	47	theme	vast	1268:1271	arg1	plasticity					1288:1297	vast conformational plasticity	1268:1297	vast conformational plasticity	1268:1297	In contrast, in the absence of glycosylation, we observed vast conformational plasticity with the functional domains frequently collapsing.					
31279935	3	48	with	variants	356:363	arg1	activity					392:399	improved cellulolytic activity	370:399	improved cellulolytic activity	370:399	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	5	49	theme	Cel7A	563:567	arg1	structure					578:586	Cel7A complete structure	563:586	Cel7A complete structure	563:586	However, the linker flexibility has hindered the determination of Cel7A complete structure.					
31279935	7	50	theme	glycosylation	756:768	arg1	effects					770:776	the glycosylation effects	752:776	the glycosylation effects on the structure and dynamics of a solubilized TrCel7A	752:831	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	3	51	theme	structure	306:314	arg1	understanding					289:301	the understanding	285:301	the understanding of structure and dynamics	285:327	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	3	51	theme	structure	306:314	arg1	crucial					332:338	crucial	332:338	crucial	332:338	Thus, the understanding of structure and dynamics is crucial for engineering variants with improved cellulolytic activity.					
31279935	2	52	theme	Trichoderma	220:230	arg1	TrCel7A					240:246	TrCel7A	240:246	TrCel7A	240:246	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	2	52	theme	Trichoderma	220:230	arg1	reesei					232:237	Trichoderma reesei	220:237	Trichoderma reesei (TrCel7A)	220:247	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	0	53	dep	structure	29:37	arg1	the					25:27	the	25:27	the	25:27	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	5	54	theme	complete	569:576	arg1	structure					578:586	Cel7A complete structure	563:586	Cel7A complete structure	563:586	However, the linker flexibility has hindered the determination of Cel7A complete structure.					
31279935	10	55	theme	concerted	1168:1176	arg1	way					1178:1180	a more concerted way	1161:1180	a more concerted way	1161:1180	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
31279935	5	56	theme	structure	578:586	arg1	determination					546:558	the determination	542:558	the determination of Cel7A complete structure	542:586	However, the linker flexibility has hindered the determination of Cel7A complete structure.					
31279935	10	57	theme	interdomain	1129:1139	arg1	motions					1141:1147	functional interdomain motions	1118:1147	functional interdomain motions	1118:1147	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
31279935	2	58	from	process	270:276	arg1	critical					253:260	critical	253:260	critical	253:260	Industrial cellulases such as Cel7A from Trichoderma reesei (TrCel7A) are critical in this process.					
31279935	8	59	theme	protein	932:938	arg1	dynamics					948:955	protein overall dynamics	932:955	protein overall dynamics	932:955	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	1	60	theme	cellulose-containing	123:142	arg1	biomass					144:150	cellulose-containing biomass	123:150	cellulose-containing biomass for bioethanol production	123:176	Fungi cellulases are used to degrade cellulose-containing biomass for bioethanol production.					
31279935	6	61	theme	enzyme	696:701	arg1	structure					703:711	the complete enzyme structure	683:711	the complete enzyme structure	683:711	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	0	62	theme	full-length	57:67	arg1	cellulase					75:83	a full-length Cel7A cellulase	55:83	a full-length Cel7A cellulase	55:83	Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase.					
31279935	10	63	gly	glycosylated	1189:1200	arg1	system					1202:1207	the glycosylated system	1185:1207	the glycosylated system	1185:1207	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
31279935	8	64	theme	dynamics	948:955	arg1	stabilization					915:927	the stabilization	911:927	the stabilization of protein overall dynamics	911:955	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	6	65	theme	integrative	641:651	arg1	modelling					653:661	integrative modelling	641:661	integrative modelling	641:661	Herein, based on atomic and sparse data, we applied integrative modelling to build a model of the complete enzyme structure.					
31279935	7	66	dep	structure	785:793	arg1	the					781:783	the	781:783	the	781:783	Next, through simulations, we studied the glycosylation effects on the structure and dynamics of a solubilized TrCel7A.					
31279935	9	67	theme	protein	992:998	arg1	angles					1009:1014	protein dihedral angles	992:1014	protein dihedral angles distribution	992:1027	O-linked glycans seem to restrict protein dihedral angles distribution in this region, selecting more elongated conformations.					
31279935	8	68	theme	overall	940:946	arg1	dynamics					948:955	protein overall dynamics	932:955	protein overall dynamics	932:955	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	8	69	theme	dynamics	844:851	arg1	analysis					853:860	Essential dynamics analysis	834:860	Essential dynamics analysis	834:860	Essential dynamics analysis showed that O-glycosylation in the linker led to the stabilization of protein overall dynamics.					
31279935	10	70	theme	glycosylated	1189:1200	arg1	system					1202:1207	the glycosylated system	1185:1207	the glycosylated system	1185:1207	Besides the reduced flexibility, functional interdomain motions occurred in a more concerted way in the glycosylated system.					
30459171	3	0	theme	human	577:581	arg1	tissues					590:596	human kidney tissues	577:596	human kidney tissues	577:596	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	1	gly	glycopeptides	428:440	arg2	glycopeptides					428:440	O-linked glycopeptides	419:440	O-linked glycopeptides (EXoO)	419:447	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	1	gly	glycopeptides	428:440	arg2	EXoO					443:446	EXoO	443:446	EXoO	443:446	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	1	2	theme	modifications	162:174	arg1	one					118:120	one	118:120	one	118:120	Protein glycosylation is one of the most abundant post-translational modifications.					
30459171	1	2	theme	modifications	162:174	arg1	modifications					162:174	the most abundant post-translational modifications	125:174	the most abundant post-translational modifications	125:174	Protein glycosylation is one of the most abundant post-translational modifications.					
30459171	5	3	theme	extracellular	926:938	arg1	space					940:944	extracellular space	926:944	extracellular space	926:944	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	3	4	theme	sites	513:517	arg1	definition					523:532	definition	523:532	definition of their glycans	523:549	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	4	theme	sites	513:517	arg1	mapping					468:474	the mapping	464:474	the mapping of over 3,000 O-linked glycosylation sites	464:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	6	5	theme	O-linked	1085:1092	arg1	glycoproteome					1094:1106	the O-linked glycoproteome	1081:1106	the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics	1081:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	5	6	theme	endoplasmic	997:1007	arg1	reticulum					1009:1017	the endoplasmic reticulum	993:1017	the endoplasmic reticulum (ER)	993:1022	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	5	6	theme	endoplasmic	997:1007	arg1	ER					1020:1021	ER	1020:1021	ER	1020:1021	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	2	7	theme	major	233:237	arg1	type					239:242	a major type	231:242	a major type of protein glycosylation	231:267	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	2	7	theme	major	233:237	arg1	glycosylation					216:228	O-linked glycosylation	207:228	O-linked glycosylation	207:228	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	0	8	gly	glycopeptides	71:83	arg2	glycopeptides					71:83	O-linked glycopeptides	62:83	O-linked glycopeptides	62:83	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	5	9	theme	structural	820:829	arg1	analysis					831:838	Detailed structural analysis	811:838	Detailed structural analysis of the sites identified	811:862	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	4	10	gly	glycosylation	661:673	arg2	sites					675:679	O-linked glycosylation sites	652:679	O-linked glycosylation sites	652:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	4	11	from	glycoproteome	767:779	arg1	types					794:798	different types	784:798	different types of sample	784:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	3	12	theme	glycosylation	499:511	arg1	sites					513:517	over 3,000 O-linked glycosylation sites	479:517	over 3,000 O-linked glycosylation sites	479:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	6	13	from	use	1167:1169	arg1	therapeutics					1199:1210	therapeutics	1199:1210	therapeutics	1199:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	6	13	from	use	1167:1169	arg1	diagnostics					1183:1193	clinical diagnostics	1174:1193	clinical diagnostics	1174:1193	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	4	14	link	O-linked	758:765	arg1	glycoproteome					767:779	the site-specific O-linked glycoproteome	740:779	the site-specific O-linked glycoproteome in different types of sample	740:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	5	15	theme	sites	847:851	arg1	analysis					831:838	Detailed structural analysis	811:838	Detailed structural analysis of the sites identified	811:862	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	4	16	theme	different	784:792	arg1	types					794:798	different types	784:798	different types of sample	784:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	6	17	theme	significant	1054:1064	arg1	differences					1066:1076	significant differences	1054:1076	significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics	1054:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	5	18	theme	conserved	873:881	arg1	motifs					883:888	conserved motifs	873:888	conserved motifs	873:888	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	3	19	theme	chemoenzymatic	347:360	arg1	method					362:367	a chemoenzymatic method	345:367	a chemoenzymatic method	345:367	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	0	20	link	O-linked	62:69	arg1	glycopeptides					71:83	O-linked glycopeptides	62:83	O-linked glycopeptides	62:83	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	3	21	gly	glycosylation	499:511	arg2	sites					513:517	over 3,000 O-linked glycosylation sites	479:517	over 3,000 O-linked glycosylation sites	479:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	21	gly	glycosylation	499:511	arg2	3,000					484:488	3,000	484:488	3,000	484:488	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	5	22	theme	Detailed	811:818	arg1	analysis					831:838	Detailed structural analysis	811:838	Detailed structural analysis of the sites identified	811:862	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	2	23	theme	suitable	315:322	arg1	methodologies					324:336	suitable methodologies	315:336	suitable methodologies	315:336	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	4	24	theme	glycosylation	661:673	arg1	sites					675:679	O-linked glycosylation sites	652:679	O-linked glycosylation sites	652:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	3	25	theme	site-specific	391:403	arg1	extraction					405:414	the site-specific extraction	387:414	the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues	387:596	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	4	26	theme	effective	710:718	arg1	EXoO					699:702	EXoO	699:702	EXoO	699:702	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	4	26	theme	effective	710:718	arg1	method					720:725	an effective method	707:725	an effective method for defining the site-specific O-linked glycoproteome in different types of sample	707:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	1	27	theme	Protein	93:99	arg1	glycosylation					101:113	Protein glycosylation	93:113	Protein glycosylation	93:113	Protein glycosylation is one of the most abundant post-translational modifications.					
30459171	4	28	theme	O-linked	652:659	arg1	sites					675:679	O-linked glycosylation sites	652:679	O-linked glycosylation sites	652:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	3	29	theme	T	599:599	arg1	cells					601:605	T cells	599:605	T cells	599:605	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	6	30	from	differences	1066:1076	arg1	glycoproteome					1094:1106	the O-linked glycoproteome	1081:1106	the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics	1081:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	4	31	theme	site-specific	744:756	arg1	glycoproteome					767:779	the site-specific O-linked glycoproteome	740:779	the site-specific O-linked glycoproteome in different types of sample	740:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	2	32	link	O-linked	207:214	arg1	type					239:242	a major type	231:242	a major type of protein glycosylation	231:267	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	2	32	link	O-linked	207:214	arg1	glycosylation					216:228	O-linked glycosylation	207:228	O-linked glycosylation	207:228	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	4	33	theme	sample	803:808	arg1	types					794:798	different types	784:798	different types of sample	784:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	6	34	theme	clinical	1174:1181	arg1	diagnostics					1183:1193	clinical diagnostics	1174:1193	clinical diagnostics	1174:1193	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	6	35	theme	tissues	1135:1141	arg1	glycoproteome					1094:1106	the O-linked glycoproteome	1081:1106	the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics	1081:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	5	36	theme	Golgi	982:986	arg1	reticulum					1009:1017	the endoplasmic reticulum	993:1017	the endoplasmic reticulum (ER)	993:1022	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	5	36	theme	Golgi	982:986	arg1	lumen					969:973	the lumen	965:973	the lumen of the Golgi	965:986	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	5	36	theme	Golgi	982:986	arg1	space					940:944	extracellular space	926:944	extracellular space	926:944	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	5	36	theme	Golgi	982:986	arg1	ER					1020:1021	ER	1020:1021	ER	1020:1021	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	5	36	theme	Golgi	982:986	arg1	surface					956:962	the cell surface	947:962	the cell surface	947:962	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	3	37	theme	O-linked	419:426	arg1	EXoO					443:446	EXoO	443:446	EXoO	443:446	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	37	theme	O-linked	419:426	arg1	glycopeptides					428:440	O-linked glycopeptides	419:440	O-linked glycopeptides (EXoO)	419:447	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	6	38	theme	normal	1121:1126	arg1	tissues					1135:1141	tumor and normal kidney tissues	1111:1141	tissues	1135:1141	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	2	39	theme	detailed	186:193	arg1	analysis					195:202	detailed analysis	186:202	detailed analysis of O-linked glycosylation, a major type of protein glycosylation,	186:268	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	5	40	theme	topological	894:904	arg1	orientations					906:917	topological orientations	894:917	topological orientations	894:917	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	4	41	theme	large-scale	624:634	arg1	localization					636:647	This large-scale localization	619:647	This large-scale localization of O-linked glycosylation sites	619:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	0	42	theme	site-specific	34:46	arg1	extraction					48:57	site-specific extraction	34:57	site-specific extraction of O-linked glycopeptides	34:83	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	3	43	theme	glycopeptides	428:440	arg1	cells					601:605	T cells	599:605	T cells	599:605	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	43	theme	glycopeptides	428:440	arg1	serum					612:616	serum	612:616	serum	612:616	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	43	theme	glycopeptides	428:440	arg1	extraction					405:414	the site-specific extraction	387:414	the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues	387:596	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	4	44	theme	O-linked	758:765	arg1	glycoproteome					767:779	the site-specific O-linked glycoproteome	740:779	the site-specific O-linked glycoproteome in different types of sample	740:808	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	4	45	link	O-linked	652:659	arg1	sites					675:679	O-linked glycosylation sites	652:679	O-linked glycosylation sites	652:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	0	46	theme	O-linked	62:69	arg1	glycopeptides					71:83	O-linked glycopeptides	62:83	O-linked glycopeptides	62:83	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	3	47	link	O-linked	419:426	arg1	EXoO					443:446	EXoO	443:446	EXoO	443:446	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	47	link	O-linked	419:426	arg1	glycopeptides					428:440	O-linked glycopeptides	419:440	O-linked glycopeptides (EXoO)	419:447	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	6	48	theme	kidney	1128:1133	arg1	tissues					1135:1141	tumor and normal kidney tissues	1111:1141	tissues	1135:1141	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	6	49	link	O-linked	1085:1092	arg1	glycoproteome					1094:1106	the O-linked glycoproteome	1081:1106	the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics	1081:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	3	50	theme	kidney	583:588	arg1	tissues					590:596	human kidney tissues	577:596	human kidney tissues	577:596	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	51	theme	O-linked	490:497	arg1	sites					513:517	over 3,000 O-linked glycosylation sites	479:517	over 3,000 O-linked glycosylation sites	479:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	0	52	dep	Mapping	0:6	arg1	EXoO					86:89	EXoO	86:89	EXoO	86:89	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	2	53	theme	glycosylation	216:228	arg1	analysis					195:202	detailed analysis	186:202	detailed analysis of O-linked glycosylation, a major type of protein glycosylation,	186:268	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	4	54	theme	sites	675:679	arg1	localization					636:647	This large-scale localization	619:647	This large-scale localization of O-linked glycosylation sites	619:679	This large-scale localization of O-linked glycosylation sites demonstrated that EXoO is an effective method for defining the site-specific O-linked glycoproteome in different types of sample.					
30459171	5	55	theme	cell	951:954	arg1	surface					956:962	the cell surface	947:962	the cell surface	947:962	Detailed structural analysis of the sites identified revealed conserved motifs and topological orientations facing extracellular space, the cell surface, the lumen of the Golgi, and the endoplasmic reticulum (ER).					
30459171	6	56	theme	tumor	1111:1115	arg1	tissues					1135:1141	tumor and normal kidney tissues	1111:1141	tissues	1135:1141	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	3	57	link	O-linked	490:497	arg1	sites					513:517	over 3,000 O-linked glycosylation sites	479:517	over 3,000 O-linked glycosylation sites	479:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	2	58	theme	O-linked	207:214	arg1	type					239:242	a major type	231:242	a major type of protein glycosylation	231:267	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	2	58	theme	O-linked	207:214	arg1	glycosylation					216:228	O-linked glycosylation	207:228	O-linked glycosylation	207:228	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	3	59	theme	glycans	543:549	arg1	definition					523:532	definition	523:532	definition of their glycans	523:549	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	3	59	theme	glycans	543:549	arg1	mapping					468:474	the mapping	464:474	the mapping of over 3,000 O-linked glycosylation sites	464:517	Here, a chemoenzymatic method is introduced for the site-specific extraction of O-linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O-linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum.					
30459171	2	60	theme	methodologies	324:336	arg1	scarcity					303:310	the scarcity	299:310	the scarcity of suitable methodologies	299:336	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	2	61	theme	glycosylation	255:267	arg1	type					239:242	a major type	231:242	a major type of protein glycosylation	231:267	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	2	61	theme	glycosylation	255:267	arg1	glycosylation					216:228	O-linked glycosylation	207:228	O-linked glycosylation	207:228	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	6	62	theme	broader	1159:1165	arg1	use					1167:1169	its broader use	1155:1169	its broader use in clinical diagnostics and therapeutics	1155:1210	EXoO was also able to reveal significant differences in the O-linked glycoproteome of tumor and normal kidney tissues pointing to its broader use in clinical diagnostics and therapeutics.					
30459171	1	63	theme	abundant	134:141	arg1	modifications					162:174	the most abundant post-translational modifications	125:174	the most abundant post-translational modifications	125:174	Protein glycosylation is one of the most abundant post-translational modifications.					
30459171	0	64	theme	glycopeptides	71:83	arg1	extraction					48:57	site-specific extraction	34:57	site-specific extraction of O-linked glycopeptides	34:83	Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO).					
30459171	2	65	theme	protein	247:253	arg1	glycosylation					255:267	protein glycosylation	247:267	protein glycosylation	247:267	However, detailed analysis of O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of suitable methodologies.					
30459171	1	66	theme	post-translational	143:160	arg1	modifications					162:174	the most abundant post-translational modifications	125:174	the most abundant post-translational modifications	125:174	Protein glycosylation is one of the most abundant post-translational modifications.					
30689864	7	0	theme	HIV	1307:1309	arg1	protein					1320:1326	HIV envelope protein	1307:1326	HIV envelope protein	1307:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	4	1	theme	previous	558:565	arg1	study					567:571	our previous study	554:571	our previous study	554:571	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	3	2	gly	N-/O-glycosylation	465:482	arg1	systems					542:548	carbohydrate-only systems	524:548	carbohydrate-only systems	524:548	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	2	gly	N-/O-glycosylation	465:482	arg1	protein					498:504	the target protein	487:504	the target protein	487:504	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	5	3	theme	glycan	946:951	arg1	structures					962:971	PDB glycan template structures	942:971	PDB glycan template structures	942:971	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	4	4	theme	carbohydrate	633:644	arg1	molecules					646:654	carbohydrate molecules	633:654	carbohydrate molecules	633:654	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	0	5	theme	glycoconjugates	75:89	arg1	simulation					43:52	simulation	43:52	simulation	43:52	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	0	5	theme	glycoconjugates	75:89	arg1	modeling					30:37	modeling	30:37	modeling	30:37	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	10	6	from	http	1714:1717	arg1	available					1701:1709	available	1701:1709	available	1701:1709	Glycan Reader & Modeler is freely available at http://charmm-gui.org/input/glycan.					
30689864	7	7	theme	structural	1206:1215	arg1	properties					1217:1226	the structural properties	1202:1226	the structural properties of modeled glycan structures	1202:1255	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	9	8	theme	glycoconjugate	1557:1570	arg1	modeling					1572:1579	glycoconjugate modeling	1557:1579	glycoconjugate modeling with video demos	1557:1596	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	5	9	theme	template	953:960	arg1	structures					962:971	PDB glycan template structures	942:971	PDB glycan template structures	942:971	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	5	10	theme	Glycan	778:783	arg1	Modeler					810:816	Modeler	810:816	Modeler	810:816	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	5	10	theme	Glycan	778:783	arg1	Modeler					785:791	Glycan Modeler	778:791	Glycan Modeler (Glycan Reader & Modeler)	778:817	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	2	11	theme	essential	343:351	arg1	tool					353:356	an essential tool	340:356	an essential tool complementary to experimental methods	340:394	Computational modeling and molecular simulation have become an essential tool complementary to experimental methods.					
30689864	10	12	dep	http	1714:1717	arg1	//charmm-gui.org/input/glycan					1719:1747	//charmm-gui.org/input/glycan	1719:1747	http://charmm-gui.org/input/glycan	1714:1747	Glycan Reader & Modeler is freely available at http://charmm-gui.org/input/glycan.					
30689864	3	13	theme	computational	416:428	arg1	tool					430:433	a computational tool	414:433	a computational tool	414:433	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	13	theme	computational	416:428	arg1	Modeler					443:449	Glycan Modeler	436:449	Glycan Modeler	436:449	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	9	14	theme	practical	1616:1624	arg1	applications					1626:1637	the practical applications	1612:1637	the practical applications of Glycan Reader & Modeler	1612:1664	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	5	15	gly	glycosylation	916:928	arg2	sites					930:934	glycosylation sites	916:934	glycosylation sites	916:934	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	5	16	dep	Database	994:1001	arg1	http					1004:1007	http	1004:1007	http://glycanstructure.org/fragment-db	1004:1041	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	9	17	theme	modeling	1572:1579	arg1	examples					1545:1552	the representative examples	1526:1552	the representative examples of glycoconjugate modeling with video demos	1526:1596	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	5	18	theme	Fragment	985:992	arg1	Database					994:1001	Glycan Fragment Database	978:1001	Glycan Fragment Database (http://glycanstructure.org/fragment-db)	978:1042	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	7	19	theme	molecular	1273:1281	arg1	simulations					1292:1302	2-μs molecular dynamics simulations	1268:1302	2-μs molecular dynamics simulations of HIV envelope protein	1268:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	6	20	theme	Modeler	1124:1130	arg1	applicability					1091:1103	the universal applicability	1077:1103	the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins	1077:1178	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
30689864	6	21	theme	glycan	1143:1148	arg1	sequences					1150:1158	various glycan sequences	1135:1158	various glycan sequences	1135:1158	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
30689864	3	22	theme	target	491:496	arg1	protein					498:504	the target protein	487:504	the target protein	487:504	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	6	23	theme	benchmark	1049:1057	arg1	tests					1059:1063	Our benchmark tests	1045:1063	Our benchmark tests	1045:1063	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
30689864	3	24	dep	in	455:456	arg1	silico					458:463	silico	458:463	silico	458:463	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	5	25	dep	http	1004:1007	arg1	//glycanstructure.org/fragment-db					1009:1041	//glycanstructure.org/fragment-db	1009:1041	http://glycanstructure.org/fragment-db	1004:1041	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	7	26	theme	2-μs	1268:1271	arg1	simulations					1292:1302	2-μs molecular dynamics simulations	1268:1302	2-μs molecular dynamics simulations of HIV envelope protein	1268:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	3	27	theme	protein	498:504	arg1	generation					510:519	generation	510:519	generation of carbohydrate-only systems	510:548	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	27	theme	protein	498:504	arg1	N-/O-glycosylation					465:482	in silico N-/O-glycosylation	455:482	in silico N-/O-glycosylation of the target protein	455:504	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	28	theme	Glycan	436:441	arg1	tool					430:433	a computational tool	414:433	a computational tool	414:433	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	28	theme	Glycan	436:441	arg1	Modeler					443:449	Glycan Modeler	436:449	Glycan Modeler	436:449	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	7	29	theme	envelope	1311:1318	arg1	protein					1320:1326	HIV envelope protein	1307:1326	HIV envelope protein	1307:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	9	30	theme	Glycan	1642:1647	arg1	Modeler					1658:1664	Glycan Reader & Modeler	1642:1664	Glycan Reader & Modeler	1642:1664	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	2	31	theme	experimental	375:386	arg1	methods					388:394	experimental methods	375:394	experimental methods	375:394	Computational modeling and molecular simulation have become an essential tool complementary to experimental methods.					
30689864	5	32	theme	glycosylation	916:928	arg1	sites					930:934	glycosylation sites	916:934	glycosylation sites	916:934	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	0	33	theme	CHARMM-GUI	0:9	arg1	Modeler					18:24	CHARMM-GUI Glycan Modeler	0:24	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.	0:90	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	4	34	theme	web-based	604:612	arg1	tool					614:617	a web-based tool	602:617	a web-based tool for detecting carbohydrate molecules from a PDB structure	602:675	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	5	35	theme	PDB	942:944	arg1	structures					962:971	PDB glycan template structures	942:971	PDB glycan template structures	942:971	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	1	36	theme	biological	218:227	arg1	roles					229:233	their biological roles	212:233	their biological roles	212:233	Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents.					
30689864	5	37	theme	glycans	857:863	arg1	structures					843:852	the structures	839:852	the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db)	839:1042	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	8	38	contain	have	1418:1421	arg2	features					1446:1453	the similar structural features	1423:1453	the similar structural features	1423:1453	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	8	38	contain	have	1418:1421	arg1	structures					1374:1383	the modeled glycan structures	1355:1383	the modeled glycan structures built by Glycan Reader & Modeler	1355:1416	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	9	39	theme	representative	1530:1543	arg1	examples					1545:1552	the representative examples	1526:1552	the representative examples of glycoconjugate modeling with video demos	1526:1596	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	2	40	theme	complementary	358:370	arg1	tool					353:356	an essential tool	340:356	an essential tool complementary to experimental methods	340:394	Computational modeling and molecular simulation have become an essential tool complementary to experimental methods.					
30689864	8	41	theme	X-ray	1486:1490	arg1	crystallography					1492:1506	X-ray crystallography	1486:1506	X-ray crystallography	1486:1506	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	5	42	theme	glycoconjugates	869:883	arg1	structures					843:852	the structures	839:852	the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db)	839:1042	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	4	43	theme	PDB	663:665	arg1	structure					667:675	a PDB structure	661:675	a PDB structure	661:675	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	6	44	theme	target	1164:1169	arg1	proteins					1171:1178	target proteins	1164:1178	target proteins	1164:1178	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
30689864	9	45	theme	Reader	1649:1654	arg1	Modeler					1658:1664	Glycan Reader & Modeler	1642:1664	Glycan Reader & Modeler	1642:1664	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	3	46	theme	carbohydrate-only	524:540	arg1	systems					542:548	carbohydrate-only systems	524:548	carbohydrate-only systems	524:548	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	7	47	theme	modeled	1231:1237	arg1	structures					1246:1255	modeled glycan structures	1231:1255	modeled glycan structures	1231:1255	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	9	48	with	modeling	1572:1579	arg1	demos					1592:1596	video demos	1586:1596	video demos	1586:1596	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	5	49	theme	given	889:893	arg1	sequences					902:910	given glycan sequences	889:910	given glycan sequences	889:910	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	2	50	theme	Computational	280:292	arg1	modeling					294:301	Computational modeling	280:301	Computational modeling	280:301	Computational modeling and molecular simulation have become an essential tool complementary to experimental methods.					
30689864	4	51	theme	files	723:727	arg1	Reader					594:599	Glycan Reader	587:599	Glycan Reader	587:599	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	51	theme	files	723:727	arg1	generation					681:690	generation	681:690	generation of simulation system and input files	681:727	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	51	theme	files	723:727	arg1	tool					614:617	a web-based tool	602:617	a web-based tool for detecting carbohydrate molecules from a PDB structure	602:675	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	52	theme	system	706:711	arg1	Reader					594:599	Glycan Reader	587:599	Glycan Reader	587:599	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	52	theme	system	706:711	arg1	generation					681:690	generation	681:690	generation of simulation system and input files	681:727	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	52	theme	system	706:711	arg1	tool					614:617	a web-based tool	602:617	a web-based tool for detecting carbohydrate molecules from a PDB structure	602:675	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	53	theme	input	717:721	arg1	files					723:727	input files	717:727	input files	717:727	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	4	54	theme	Glycan	587:592	arg1	Reader					594:599	Glycan Reader	587:599	Glycan Reader	587:599	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	7	55	theme	glycan	1239:1244	arg1	structures					1246:1255	modeled glycan structures	1231:1255	modeled glycan structures	1231:1255	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	7	56	theme	dynamics	1283:1290	arg1	simulations					1292:1302	2-μs molecular dynamics simulations	1268:1302	2-μs molecular dynamics simulations of HIV envelope protein	1268:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	5	57	theme	glycan	895:900	arg1	sequences					902:910	given glycan sequences	889:910	given glycan sequences	889:910	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	1	58	theme	efficient	250:258	arg1	agents					272:277	efficient therapeutic agents	250:277	efficient therapeutic agents	250:277	Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents.					
30689864	8	59	theme	structural	1435:1444	arg1	features					1446:1453	the similar structural features	1423:1453	the similar structural features	1423:1453	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	9	60	theme	video	1586:1590	arg1	demos					1592:1596	video demos	1586:1596	video demos	1586:1596	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	3	61	theme	in	455:456	arg1	N-/O-glycosylation					465:482	in silico N-/O-glycosylation	455:482	in silico N-/O-glycosylation of the target protein	455:504	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	6	62	theme	various	1135:1141	arg1	sequences					1150:1158	various glycan sequences	1135:1158	various glycan sequences	1135:1158	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
30689864	9	63	theme	&	1656:1656	arg1	Modeler					1658:1664	Glycan Reader & Modeler	1642:1664	Glycan Reader & Modeler	1642:1664	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	7	64	theme	structures	1246:1255	arg1	properties					1217:1226	the structural properties	1202:1226	the structural properties of modeled glycan structures	1202:1255	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	3	65	theme	systems	542:548	arg1	generation					510:519	generation	510:519	generation of carbohydrate-only systems	510:548	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	3	65	theme	systems	542:548	arg1	N-/O-glycosylation					465:482	in silico N-/O-glycosylation	455:482	in silico N-/O-glycosylation of the target protein	455:504	Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems.					
30689864	1	66	theme	therapeutic	260:270	arg1	agents					272:277	efficient therapeutic agents	250:277	efficient therapeutic agents	250:277	Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents.					
30689864	5	67	theme	Glycan	749:754	arg1	Reader					756:761	Glycan Reader	749:761	Glycan Reader	749:761	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	2	68	theme	molecular	307:315	arg1	simulation					317:326	molecular simulation	307:326	molecular simulation	307:326	Computational modeling and molecular simulation have become an essential tool complementary to experimental methods.					
30689864	1	69	theme	structures	171:180	arg1	context					142:148	the context	138:148	the context of three-dimensional structures	138:180	Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents.					
30689864	8	70	theme	similar	1427:1433	arg1	features					1446:1453	the similar structural features	1423:1453	the similar structural features	1423:1453	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	5	71	theme	Glycan	978:983	arg1	Database					994:1001	Glycan Fragment Database	978:1001	Glycan Fragment Database (http://glycanstructure.org/fragment-db)	978:1042	As integrated into Glycan Reader in CHARMM-GUI, Glycan Modeler (Glycan Reader & Modeler) enables to generate the structures of glycans and glycoconjugates for given glycan sequences and glycosylation sites using PDB glycan template structures from Glycan Fragment Database (http://glycanstructure.org/fragment-db).					
30689864	7	72	theme	protein	1320:1326	arg1	simulations					1292:1302	2-μs molecular dynamics simulations	1268:1302	2-μs molecular dynamics simulations of HIV envelope protein	1268:1326	We also investigated the structural properties of modeled glycan structures by running 2-μs molecular dynamics simulations of HIV envelope protein.					
30689864	4	73	theme	simulation	695:704	arg1	system					706:711	simulation system	695:711	simulation system	695:711	In our previous study, we developed Glycan Reader, a web-based tool for detecting carbohydrate molecules from a PDB structure and generation of simulation system and input files.					
30689864	8	74	theme	glycan	1367:1372	arg1	structures					1374:1383	the modeled glycan structures	1355:1383	the modeled glycan structures built by Glycan Reader & Modeler	1355:1416	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	8	75	theme	modeled	1359:1365	arg1	structures					1374:1383	the modeled glycan structures	1355:1383	the modeled glycan structures built by Glycan Reader & Modeler	1355:1416	The simulations show that the modeled glycan structures built by Glycan Reader & Modeler have the similar structural features compared to the ones solved by X-ray crystallography.					
30689864	1	76	theme	three-dimensional	153:169	arg1	structures					171:180	three-dimensional structures	153:180	three-dimensional structures	153:180	Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents.					
30689864	9	77	theme	Modeler	1658:1664	arg1	applications					1626:1637	the practical applications	1612:1637	the practical applications of Glycan Reader & Modeler	1612:1664	We also describe the representative examples of glycoconjugate modeling with video demos to illustrate the practical applications of Glycan Reader & Modeler.					
30689864	0	78	theme	carbohydrates	57:69	arg1	simulation					43:52	simulation	43:52	simulation	43:52	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	0	78	theme	carbohydrates	57:69	arg1	modeling					30:37	modeling	30:37	modeling	30:37	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	0	79	theme	Glycan	11:16	arg1	Modeler					18:24	CHARMM-GUI Glycan Modeler	0:24	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.	0:90	CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.					
30689864	6	80	theme	universal	1081:1089	arg1	applicability					1091:1103	the universal applicability	1077:1103	the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins	1077:1178	Our benchmark tests demonstrate the universal applicability of Glycan Reader & Modeler to various glycan sequences and target proteins.					
29466327	1	0	gly	glycoproteins	183:195	arg1	glycoproteins					183:195	glycoproteins	183:195	glycoproteins	183:195	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	4	1	dep	subunits	623:630	arg1	Stt3					643:646	Stt3	643:646	Stt3	643:646	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	4	1	dep	subunits	623:630	arg1	Ost1-Ost5					632:640	Ost1-Ost5	632:640	Ost1-Ost5	632:640	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	4	1	dep	subunits	623:630	arg1	Swp1					658:661	Swp1	658:661	Swp1	658:661	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	4	1	dep	subunits	623:630	arg1	Wbp1					649:652	Wbp1	649:652	Wbp1	649:652	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	4	1	dep	subunits	623:630	arg1	subunits					623:630	subunits Ost1-Ost5, Stt3, Wbp1 and Swp1	623:661	subunits Ost1-Ost5, Stt3, Wbp1 and Swp1	623:661	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	3	2	theme	protein	391:397	arg1	N-glycosylation					399:413	eukaryotic protein N-glycosylation	380:413	eukaryotic protein N-glycosylation	380:413	Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures.					
29466327	4	3	theme	Saccharomyces	557:569	arg1	complex					586:592	the Saccharomyces cerevisiae OST complex	553:592	the Saccharomyces cerevisiae OST complex	553:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	1	4	gly	N-glycosylated	201:214	arg1	glycoproteins					183:195	glycoproteins	183:195	glycoproteins	183:195	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	1	4	gly	N-glycosylated	201:214	arg1	%					178:178	about 90%	170:178	about 90% of glycoproteins	170:195	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	2	5	from	membrane	349:356	arg1	complex					295:301	an eight-protein oligosaccharyltransferase (OST) complex	246:301	an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane	246:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	2	5	from	membrane	349:356	arg1	embedded					311:318	embedded	311:318	embedded	311:318	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	6	6	theme	catalytic	932:940	arg1	site					942:945	the OST catalytic site	924:945	the OST catalytic site	924:945	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	2	7	theme	reticulum	339:347	arg1	membrane					349:356	the endoplasmic reticulum membrane	323:356	the endoplasmic reticulum membrane	323:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	3	8	theme	N-glycosylation	399:413	arg1	understanding					363:375	Our understanding	359:375	Our understanding of eukaryotic protein N-glycosylation	359:413	Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures.					
29466327	4	9	theme	cryo-electron	515:527	arg1	microscopy					529:538	3.5 Å resolution cryo-electron microscopy	498:538	a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex	496:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	4	10	theme	resolution	504:513	arg1	microscopy					529:538	3.5 Å resolution cryo-electron microscopy	498:538	a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex	496:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	3	11	theme	eukaryotic	380:389	arg1	N-glycosylation					399:413	eukaryotic protein N-glycosylation	380:413	eukaryotic protein N-glycosylation	380:413	Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures.					
29466327	1	12	theme	glycoproteins	183:195	arg1	glycoproteins					183:195	glycoproteins	183:195	glycoproteins	183:195	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	1	12	theme	glycoproteins	183:195	arg1	%					178:178	about 90%	170:178	about 90% of glycoproteins	170:195	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	0	13	theme	atomic	4:9	arg1	structure					11:19	The atomic structure	0:19	The atomic structure of a eukaryotic oligosaccharyltransferase complex	0:69	The atomic structure of a eukaryotic oligosaccharyltransferase complex.					
29466327	4	14	theme	microscopy	529:538	arg1	structure					540:548	a 3.5 Å resolution cryo-electron microscopy structure	496:548	a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex	496:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	1	15	theme	ubiquitous	93:102	arg1	modification					104:115	a ubiquitous modification	91:115	a ubiquitous modification of eukaryotic secretory and membrane-bound proteins	91:167	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	1	15	theme	ubiquitous	93:102	arg1	N-glycosylation					72:86	N-glycosylation	72:86	N-glycosylation	72:86	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	6	16	theme	OST	928:930	arg1	site					942:945	the OST catalytic site	924:945	the OST catalytic site	924:945	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	3	17	theme	high-resolution	453:467	arg1	structures					469:478	high-resolution structures	453:478	high-resolution structures	453:478	Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures.					
29466327	2	18	theme	endoplasmic	327:337	arg1	reticulum					339:347	the endoplasmic reticulum	323:347	the endoplasmic reticulum membrane	323:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	3	19	theme	structures	469:478	arg1	lack					445:448	the lack	441:448	the lack of high-resolution structures	441:478	Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures.					
29466327	7	20	theme	inhibitors	1131:1140	arg1	development					1101:1111	the development	1097:1111	the development of small-molecule inhibitors that target this process	1097:1165	The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.					
29466327	4	21	theme	subunits	623:630	arg1	structures					609:618	the structures	605:618	the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1	605:661	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	1	22	theme	eukaryotic	120:129	arg1	proteins					160:167	eukaryotic secretory and membrane-bound proteins	120:167	eukaryotic secretory and membrane-bound proteins	120:167	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	0	23	theme	eukaryotic	26:35	arg1	complex					63:69	a eukaryotic oligosaccharyltransferase complex	24:69	a eukaryotic oligosaccharyltransferase complex	24:69	The atomic structure of a eukaryotic oligosaccharyltransferase complex.					
29466327	5	24	theme	Stt3	753:756	arg1	N-glycan					758:765	an Stt3 N-glycan	750:765	an Stt3 N-glycan	750:765	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	6	25	theme	acceptor	899:906	arg1	peptide					962:968	the nascent peptide	950:968	the nascent peptide emerges from the translocon	950:996	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	6	25	theme	acceptor	899:906	arg1	peptide					908:914	the acceptor peptide	895:914	the acceptor peptide towards the OST catalytic site	895:945	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	2	26	theme	eight-protein	249:261	arg1	OST					290:292	OST	290:292	OST	290:292	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	2	26	theme	eight-protein	249:261	arg1	oligosaccharyltransferase					263:287	eight-protein oligosaccharyltransferase	249:287	an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane	246:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	4	27	theme	complex	586:592	arg1	structure					540:548	a 3.5 Å resolution cryo-electron microscopy structure	496:548	a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex	496:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	7	28	theme	small-molecule	1116:1129	arg1	inhibitors					1131:1140	small-molecule inhibitors	1116:1140	small-molecule inhibitors that target this process	1116:1165	The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.					
29466327	4	29	theme	3.5 Å	498:502	arg1	microscopy					529:538	3.5 Å resolution cryo-electron microscopy	498:538	a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex	496:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	0	30	theme	complex	63:69	arg1	structure					11:19	The atomic structure	0:19	The atomic structure of a eukaryotic oligosaccharyltransferase complex	0:69	The atomic structure of a eukaryotic oligosaccharyltransferase complex.					
29466327	4	31	theme	OST	582:584	arg1	complex					586:592	the Saccharomyces cerevisiae OST complex	553:592	the Saccharomyces cerevisiae OST complex	553:592	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
29466327	6	32	dep	peptide	962:968	arg1	emerges					970:976	emerges	970:976	emerges from the translocon	970:996	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	1	33	theme	secretory	131:139	arg1	proteins					160:167	eukaryotic secretory and membrane-bound proteins	120:167	eukaryotic secretory and membrane-bound proteins	120:167	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	0	34	theme	oligosaccharyltransferase	37:61	arg1	complex					63:69	a eukaryotic oligosaccharyltransferase complex	24:69	a eukaryotic oligosaccharyltransferase complex	24:69	The atomic structure of a eukaryotic oligosaccharyltransferase complex.					
29466327	5	35	theme	inter-subunit	718:730	arg1	interactions					732:743	the inter-subunit interactions	714:743	the inter-subunit interactions	714:743	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	5	36	with	interactions	776:787	arg1	Swp1					803:806	Swp1	803:806	Swp1	803:806	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	5	36	with	interactions	776:787	arg1	Wbp1					794:797	Wbp1	794:797	Wbp1	794:797	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	7	37	theme	protein	1053:1059	arg1	N-glycosylation					1061:1075	co-translational protein N-glycosylation	1036:1075	co-translational protein N-glycosylation	1036:1075	The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.					
29466327	5	38	theme	interactions	732:743	arg1	interactions					732:743	the inter-subunit interactions	714:743	the inter-subunit interactions	714:743	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	5	38	theme	interactions	732:743	arg1	many					706:709	many	706:709	many	706:709	We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen.					
29466327	1	39	theme	membrane-bound	145:158	arg1	proteins					160:167	eukaryotic secretory and membrane-bound proteins	120:167	eukaryotic secretory and membrane-bound proteins	120:167	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	6	40	theme	translocon	862:871	arg1	interface					873:881	the OST-Sec61 translocon interface	848:881	the OST-Sec61 translocon interface	848:881	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	1	41	theme	proteins	160:167	arg1	modification					104:115	a ubiquitous modification	91:115	a ubiquitous modification of eukaryotic secretory and membrane-bound proteins	91:167	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	1	41	theme	proteins	160:167	arg1	N-glycosylation					72:86	N-glycosylation	72:86	N-glycosylation	72:86	N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated.					
29466327	2	42	from	embedded	311:318	arg1	membrane					349:356	the endoplasmic reticulum membrane	323:356	the endoplasmic reticulum membrane	323:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	6	43	theme	OST-Sec61	852:860	arg1	interface					873:881	the OST-Sec61 translocon interface	848:881	the OST-Sec61 translocon interface	848:881	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	7	44	theme	co-translational	1036:1051	arg1	N-glycosylation					1061:1075	co-translational protein N-glycosylation	1036:1075	co-translational protein N-glycosylation	1036:1075	The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.					
29466327	6	45	theme	nascent	954:960	arg1	peptide					962:968	the nascent peptide	950:968	the nascent peptide emerges from the translocon	950:996	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	6	45	theme	nascent	954:960	arg1	peptide					908:914	the acceptor peptide	895:914	the acceptor peptide towards the OST catalytic site	895:945	Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon.					
29466327	2	46	theme	oligosaccharyltransferase	263:287	arg1	complex					295:301	an eight-protein oligosaccharyltransferase (OST) complex	246:301	an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane	246:356	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	2	46	theme	oligosaccharyltransferase	263:287	arg1	embedded					311:318	embedded	311:318	embedded	311:318	The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane.					
29466327	4	47	dep	Saccharomyces	557:569	arg1	cerevisiae					571:580	cerevisiae	571:580	cerevisiae	571:580	Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1.					
30253927	0	0	theme	key	100:102	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	0	0	theme	key	100:102	arg1	enzyme					104:109	a key enzyme	98:109	a key enzyme for complex-type N-glycan biosynthesis	98:148	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	7	1	theme	enzymatic	1262:1270	arg1	activity					1272:1279	its enzymatic activity	1258:1279	its enzymatic activity	1258:1279	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	4	2	theme	key	766:768	arg1	step					770:773	a key step	764:773	a key step in the conversion to complex-type N-glycans	764:817	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	3	3	from	synthesis	509:517	arg1	cells					532:536	mammalian cells	522:536	mammalian cells	522:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	1	4	theme	various	177:183	arg1	functions					199:207	various physiological functions	177:207	various physiological functions	177:207	N-glycans are involved in various physiological functions and their structures diverge among different phyla and kingdoms.					
30253927	6	5	theme	bacmid	1048:1053	arg1	system					1066:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system.					
30253927	4	6	theme	larval	906:911	arg1	stages					923:928	the larval and pupal stages	902:928	the larval and pupal stages	902:928	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	1	7	theme	physiological	185:197	arg1	functions					199:207	various physiological functions	177:207	various physiological functions	177:207	N-glycans are involved in various physiological functions and their structures diverge among different phyla and kingdoms.					
30253927	9	8	theme	mammalian-like	1543:1556	arg1	synthesis					1580:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	6	9	theme	nucleopolyhedrovirus	1027:1046	arg1	system					1066:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system.					
30253927	9	10	from	ortholog	1606:1613	arg1	silkworm					1618:1625	silkworm	1618:1625	silkworm	1618:1625	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	6	11	theme	mori	1022:1025	arg1	system					1066:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system.					
30253927	4	12	theme	complex-type	796:807	arg1	N-glycans					809:817	complex-type N-glycans	796:817	complex-type N-glycans	796:817	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	4	13	theme	pupal	917:921	arg1	stages					923:928	the larval and pupal stages	902:928	the larval and pupal stages	902:928	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	2	14	theme	paucimannose-type	324:340	arg1	glycans					342:348	high mannose-type and paucimannose-type glycans	302:348	high mannose-type and paucimannose-type glycans	302:348	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	4	15	theme	candidate	649:657	arg1	GnTII					710:714	GnTII	710:714	GnTII	710:714	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	4	15	theme	candidate	649:657	arg1	gene					659:662	a candidate gene	647:662	a candidate gene	647:662	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	4	15	theme	candidate	649:657	arg1	enzyme					745:750	a Golgi-localized enzyme	727:750	a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans	727:817	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	0	16	theme	N-glycan	128:135	arg1	biosynthesis					137:148	complex-type N-glycan biosynthesis	115:148	complex-type N-glycan biosynthesis	115:148	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	5	17	theme	recombinant	944:954	arg1	GnTII					964:968	recombinant B. mori GnTII	944:968	recombinant B. mori GnTII	944:968	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	5	17	theme	recombinant	944:954	arg1	form					997:1000	a soluble form	987:1000	a soluble form using a silkworm-B	987:1019	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	7	18	theme	same	1153:1156	arg1	specificity					1168:1178	the same substrate specificity	1149:1178	the same substrate specificity as human GnTII	1149:1193	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	7	19	with	deglycosylation	1200:1214	arg1	F					1241:1241	peptide:N-glycanase F	1221:1241	peptide:N-glycanase F	1221:1241	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	3	20	theme	N-glycan	500:507	arg1	synthesis					509:517	complex-type N-glycan synthesis	487:517	complex-type N-glycan synthesis in mammalian cells	487:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	0	21	theme	complex-type	115:126	arg1	biosynthesis					137:148	complex-type N-glycan biosynthesis	115:148	complex-type N-glycan biosynthesis	115:148	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	7	22	theme	substrate	1158:1166	arg1	specificity					1168:1178	the same substrate specificity	1149:1178	the same substrate specificity as human GnTII	1149:1193	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	8	23	theme	acid	1336:1339	arg1	residues					1341:1348	the amino acid residues	1326:1348	the amino acid residues involved in catalytic activity and substrate recognition	1326:1405	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	2	24	theme	mannose-type	307:318	arg1	glycans					342:348	high mannose-type and paucimannose-type glycans	302:348	high mannose-type and paucimannose-type glycans	302:348	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	7	25	theme	N-glycanase	1229:1239	arg1	F					1241:1241	peptide:N-glycanase F	1221:1241	peptide:N-glycanase F	1221:1241	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	9	26	theme	GnTII	1600:1604	arg1	ortholog					1606:1613	the GnTII ortholog	1596:1613	the GnTII ortholog in silkworm	1596:1625	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	2	27	theme	mammalian-like	363:376	arg1	glycans					391:397	very few mammalian-like complex-type glycans	354:397	very few mammalian-like complex-type glycans	354:397	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	7	28	theme	similar	1107:1113	arg1	pH					1115:1116	similar pH and temperature dependency	1107:1143	pH	1115:1116	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	9	29	theme	complex-type	1558:1569	arg1	synthesis					1580:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	3	30	theme	homologous	449:458	arg1	proteins					440:447	proteins	440:447	proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells	440:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	2	31	theme	few	359:361	arg1	glycans					391:397	very few mammalian-like complex-type glycans	354:397	very few mammalian-like complex-type glycans	354:397	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	3	32	theme	complex-type	487:498	arg1	synthesis					509:517	complex-type N-glycan synthesis	487:517	complex-type N-glycan synthesis in mammalian cells	487:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	7	33	theme	peptide	1221:1227	arg1	F					1241:1241	peptide:N-glycanase F	1221:1241	peptide:N-glycanase F	1221:1241	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	7	34	theme	temperature	1122:1132	arg1	dependency					1134:1143	similar pH and temperature dependency	1107:1143	dependency	1134:1143	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	8	35	theme	GnTII	1319:1323	arg1	structure					1300:1308	the structure	1296:1308	the structure of human GnTII	1296:1323	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	5	36	theme	soluble	989:995	arg1	GnTII					964:968	recombinant B. mori GnTII	944:968	recombinant B. mori GnTII	944:968	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	5	36	theme	soluble	989:995	arg1	form					997:1000	a soluble form	987:1000	a soluble form using a silkworm-B	987:1019	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	8	37	theme	human	1313:1317	arg1	GnTII					1319:1323	human GnTII	1313:1323	human GnTII	1313:1323	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	0	38	theme	Bombyx	44:49	arg1	mori					51:54	Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	44:95	silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	35:95	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	8	39	theme	amino	1330:1334	arg1	residues					1341:1348	the amino acid residues	1326:1348	the amino acid residues involved in catalytic activity and substrate recognition	1326:1405	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	2	40	theme	Insect	274:279	arg1	cells					281:285	Insect cells	274:285	Insect cells	274:285	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	4	41	theme	Golgi-localized	729:743	arg1	gene					659:662	a candidate gene	647:662	a candidate gene	647:662	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	4	41	theme	Golgi-localized	729:743	arg1	enzyme					745:750	a Golgi-localized enzyme	727:750	a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans	727:817	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	1	42	theme	different	244:252	arg1	phyla					254:258	phyla	254:258	phyla	254:258	N-glycans are involved in various physiological functions and their structures diverge among different phyla and kingdoms.					
30253927	0	43	theme	silkworm	35:42	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	0	43	theme	silkworm	35:42	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	0	43	theme	silkworm	35:42	arg1	enzyme					104:109	a key enzyme	98:109	a key enzyme for complex-type N-glycan biosynthesis	98:148	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	7	44	theme	human	1183:1187	arg1	GnTII					1189:1193	human GnTII	1183:1193	human GnTII	1183:1193	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	3	45	theme	mammalian	522:530	arg1	cells					532:536	mammalian cells	522:536	mammalian cells	522:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	0	46	theme	β-1,2-N-acetylglucosaminyltransferase	56:92	arg1	mori					51:54	Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	44:95	silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	35:95	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	3	47	theme	N-glycosylation	549:563	arg1	pathway					565:571	their N-glycosylation pathway	543:571	their N-glycosylation pathway	543:571	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	9	48	theme	synthesis	1580:1588	arg1	possibility					1528:1538	the possibility	1524:1538	the possibility of mammalian-like complex-type N-glycan synthesis	1524:1588	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	3	49	theme	many	409:412	arg1	insects					414:420	many insects	409:420	many insects	409:420	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	0	50	dep	silkworm	35:42	arg1	mori					51:54	Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	44:95	silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II	35:95	Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis.					
30253927	3	51	contain	possess	422:428	arg2	genes					430:434	genes	430:434	genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells	430:536	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	3	51	contain	possess	422:428	arg1	insects					414:420	many insects	409:420	many insects	409:420	However, many insects possess genes for proteins homologous to the enzymes involved in complex-type N-glycan synthesis in mammalian cells, and their N-glycosylation pathway is incompletely understood compared with that of mammals.					
30253927	5	52	theme	B. mori	956:962	arg1	GnTII					964:968	recombinant B. mori GnTII	944:968	recombinant B. mori GnTII	944:968	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	5	52	theme	B. mori	956:962	arg1	form					997:1000	a soluble form	987:1000	a soluble form using a silkworm-B	987:1019	In addition, recombinant B. mori GnTII was expressed as a soluble form using a silkworm-B.					
30253927	8	53	with	consistent	1461:1470	arg1	properties					1491:1500	its enzymatic properties	1477:1500	its enzymatic properties	1477:1500	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	7	54	theme	recombinant	1078:1088	arg1	enzyme					1090:1095	The recombinant enzyme	1074:1095	The recombinant enzyme	1074:1095	The recombinant enzyme exhibited similar pH and temperature dependency and the same substrate specificity as human GnTII, but deglycosylation with peptide:N-glycanase F did not affect its enzymatic activity.					
30253927	2	55	theme	high	302:305	arg1	glycans					342:348	high mannose-type and paucimannose-type glycans	302:348	high mannose-type and paucimannose-type glycans	302:348	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	9	56	theme	N-glycan	1571:1578	arg1	synthesis					1580:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	mammalian-like complex-type N-glycan synthesis	1543:1588	These results raised the possibility of mammalian-like complex-type N-glycan synthesis using the GnTII ortholog in silkworm.					
30253927	4	57	theme	Bombyx	834:839	arg1	mori					841:844	Bombyx mori	834:844	silkworm Bombyx mori	825:844	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	2	58	theme	complex-type	378:389	arg1	glycans					391:397	very few mammalian-like complex-type glycans	354:397	very few mammalian-like complex-type glycans	354:397	Insect cells mainly produce high mannose-type and paucimannose-type glycans but very few mammalian-like complex-type glycans.					
30253927	8	59	theme	substrate	1385:1393	arg1	recognition					1395:1405	substrate recognition	1385:1405	substrate recognition	1385:1405	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	8	60	theme	enzymatic	1481:1489	arg1	properties					1491:1500	its enzymatic properties	1477:1500	its enzymatic properties	1477:1500	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	8	61	theme	catalytic	1362:1370	arg1	activity					1372:1379	catalytic activity	1362:1379	catalytic activity	1362:1379	Compared with the structure of human GnTII, the amino acid residues involved in catalytic activity and substrate recognition are almost fully conserved in B. mori GnTII, which is consistent with its enzymatic properties.					
30253927	4	62	from	step	770:773	arg1	conversion					782:791	the conversion	778:791	the conversion to complex-type N-glycans	778:817	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	4	63	dep	silkworm	825:832	arg1	mori					841:844	Bombyx mori	834:844	silkworm Bombyx mori	825:844	Here, we cloned a candidate gene for β-1,2-N-acetylglucosaminyltransferase II (GnTII), which is a Golgi-localized enzyme involved in a key step in the conversion to complex-type N-glycans, from silkworm Bombyx mori, and the gene was found to be expressed ubiquitously in the larval and pupal stages.					
30253927	6	64	theme	expression	1055:1064	arg1	system					1066:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system	1022:1071	mori nucleopolyhedrovirus bacmid expression system.					
29981898	0	0	theme	bisected	109:116	arg1	N-glycans					118:126	unusual intersected and bisected N-glycans	85:126	unusual intersected and bisected N-glycans	85:126	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.					
29981898	3	1	theme	GlcNAc-TI	668:676	arg1	activity					678:685	GlcNAc-TI activity	668:685	GlcNAc-TI activity	668:685	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	5	2	dep	positions	1107:1115	arg1	β-bisecting					1134:1144	β-bisecting	1134:1144	β-bisecting	1134:1144	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	2	dep	positions	1107:1115	arg1	β-intersecting					1118:1131	β-intersecting	1118:1131	β-intersecting	1118:1131	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	2	dep	positions	1107:1115	arg1	α-terminal					1150:1159	α-terminal	1150:1159	α-terminal	1150:1159	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	1	3	theme	N-acetylglucosaminyltransferase	175:205	arg1	I					207:207	N-acetylglucosaminyltransferase I	175:207	N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1)	175:226	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	6	4	theme	glycan	1416:1421	arg1	enzymes					1434:1440	key glycan processing enzymes	1412:1440	key glycan processing enzymes from the Golgi apparatus	1412:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	3	5	theme	complete	646:653	arg1	abolition					655:663	a complete abolition	644:663	a complete abolition of GlcNAc-TI activity	644:685	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	4	6	theme	detailed	823:830	arg1	structures					832:841	detailed structures	823:841	detailed structures	823:841	Previously, the compositions of N-glycans from this mutant were described, but no detailed structures.					
29981898	5	7	theme	triple	1029:1034	arg1	mutant					1036:1041	gly-14 triple mutant	1022:1041	the gly-12;gly-13;gly-14 triple mutant	1004:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	6	8	theme	key	1412:1414	arg1	enzymes					1434:1440	key glycan processing enzymes	1412:1440	key glycan processing enzymes from the Golgi apparatus	1412:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	1	9	theme	multicellular	266:278	arg1	eukaryotes					280:289	multicellular eukaryotes	266:289	multicellular eukaryotes	266:289	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	2	10	theme	model	504:508	arg1	elegans					534:540	the model nematode Caenorhabditis elegans	500:540	the model nematode Caenorhabditis elegans	500:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	5	11	theme	isomeric	1165:1172	arg1	forms					1174:1178	isomeric forms	1165:1178	isomeric forms of Hex4-8HexNAc2 structures	1165:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	1	12	theme	eukaryotes	280:289	arg1	hallmark					254:261	a hallmark	252:261	a hallmark of multicellular eukaryotes	252:289	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	1	12	theme	eukaryotes	280:289	arg1	modification					133:144	The modification	129:144	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1)	129:226	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	0	13	theme	N-glycans	118:126	arg1	expression					71:80	expression	71:80	expression of unusual intersected and bisected N-glycans	71:126	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.					
29981898	1	14	theme	unicellular	349:359	arg1	organisms					361:369	unicellular organisms	349:369	unicellular organisms	349:369	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	4	15	from	mutant	793:798	arg1	compositions					757:768	the compositions	753:768	the compositions of N-glycans from this mutant	753:798	Previously, the compositions of N-glycans from this mutant were described, but no detailed structures.					
29981898	5	16	from	positions	1107:1115	arg1	forms					1174:1178	isomeric forms	1165:1178	isomeric forms of Hex4-8HexNAc2 structures	1165:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	17	theme	HPLC-MALDI-TOF-MS	862:878	arg1	approach					880:887	an off-line HPLC-MALDI-TOF-MS approach	850:887	an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS	850:937	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	6	18	from	apparatus	1457:1465	arg1	enzymes					1434:1440	key glycan processing enzymes	1412:1440	key glycan processing enzymes from the Golgi apparatus	1412:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	18	from	apparatus	1457:1465	arg1	absence					1401:1407	the absence	1397:1407	the absence of key glycan processing enzymes from the Golgi apparatus	1397:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	19	theme	large	1362:1366	arg1	degree					1368:1373	a large degree	1360:1373	a large degree of plasticity	1360:1387	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	3	20	theme	activity	678:685	arg1	abolition					655:663	a complete abolition	644:663	a complete abolition of GlcNAc-TI activity	644:685	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	1	21	located	found	300:304	arg1	metazoans					313:321	metazoans	313:321	metazoans	313:321	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	1	21	located	found	300:304	arg2	it					294:295	it	294:295	it	294:295	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	5	22	dep	gly-12	1008:1013	arg1	mutant					1036:1041	gly-14 triple mutant	1022:1041	the gly-12;gly-13;gly-14 triple mutant	1004:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	22	dep	gly-12	1008:1013	arg1	gly-13					1015:1020	gly-13	1015:1020	gly-13	1015:1020	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	2	23	theme	complex	439:445	arg1	forms					466:470	either complex or paucimannosidic forms	432:470	either complex or paucimannosidic forms	432:470	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	1	24	dep	metazoans	313:321	arg1	all					309:311	all	309:311	all	309:311	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	3	25	theme	triple	716:721	arg1	strain					733:738	a triple knock-out strain	714:738	a triple knock-out strain	714:738	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	1	26	dep	I	207:207	arg1	MGAT1					221:225	MGAT1	221:225	MGAT1	221:225	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	1	26	dep	I	207:207	arg1	GlcNAc-TI					210:218	GlcNAc-TI	210:218	GlcNAc-TI	210:218	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	3	27	theme	knock-out	723:731	arg1	strain					733:738	a triple knock-out strain	714:738	a triple knock-out strain	714:738	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	6	28	theme	Caenorhabditis	1304:1317	arg1	wider					1327:1331	wider	1327:1331	wider	1327:1331	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	28	theme	Caenorhabditis	1304:1317	arg1	repertoire					1290:1299	the N-glycomic repertoire	1275:1299	the N-glycomic repertoire of Caenorhabditis	1275:1317	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	28	theme	Caenorhabditis	1304:1317	arg1	Caenorhabditis					1304:1317	Caenorhabditis	1304:1317	Caenorhabditis	1304:1317	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	5	29	theme	N-glycans	991:999	arg1	residues					975:982	the multiple hexose residues	955:982	the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant	955:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	29	theme	N-glycans	991:999	arg1	N-glycans					991:999	the N-glycans	987:999	the N-glycans of the gly-12;gly-13;gly-14 triple mutant	987:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	29	theme	N-glycans	991:999	arg1	mannose					1056:1062	mannose	1056:1062	mannose	1056:1062	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	3	30	dep	genes	598:602	arg1	gly-14					624:629	gly-14	624:629	gly-14	624:629	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	30	dep	genes	598:602	arg1	gly-13					613:618	gly-13	613:618	gly-13	613:618	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	30	dep	genes	598:602	arg1	gly-12					605:610	gly-12	605:610	gly-12	605:610	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	30	dep	genes	598:602	arg1	genes					598:602	three different GlcNAc-TI genes	572:602	three different GlcNAc-TI genes (gly-12, gly-13 and gly-14)	572:630	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	2	31	theme	N-glycans	419:427	arg1	processing					405:414	the normal processing	394:414	the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans	394:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	5	32	from	forms	1174:1178	arg1	galactoses					1077:1086	galactoses	1077:1086	galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures	1077:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	33	gly	fucosylated	1238:1248	arg1	some					1209:1212	some	1209:1212	some	1209:1212	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	33	gly	fucosylated	1238:1248	arg1	structures					1223:1232	these structures	1217:1232	these structures	1217:1232	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	6	34	theme	plasticity	1378:1387	arg1	degree					1368:1373	a large degree	1360:1373	a large degree of plasticity	1360:1387	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	2	35	theme	Caenorhabditis	519:532	arg1	elegans					534:540	the model nematode Caenorhabditis elegans	500:540	the model nematode Caenorhabditis elegans	500:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	3	36	theme	different	578:586	arg1	gly-14					624:629	gly-14	624:629	gly-14	624:629	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	36	theme	different	578:586	arg1	gly-13					613:618	gly-13	613:618	gly-13	613:618	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	36	theme	different	578:586	arg1	gly-12					605:610	gly-12	605:610	gly-12	605:610	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	36	theme	different	578:586	arg1	genes					598:602	three different GlcNAc-TI genes	572:602	three different GlcNAc-TI genes (gly-12, gly-13 and gly-14)	572:630	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	2	37	theme	normal	398:403	arg1	processing					405:414	the normal processing	394:414	the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans	394:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	5	38	theme	different	1097:1105	arg1	positions					1107:1115	three different positions	1091:1115	three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures	1091:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	2	39	theme	nematode	510:517	arg1	elegans					534:540	the model nematode Caenorhabditis elegans	500:540	the model nematode Caenorhabditis elegans	500:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	6	40	from	absence	1401:1407	arg1	apparatus					1457:1465	the Golgi apparatus	1447:1465	the Golgi apparatus	1447:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	3	41	theme	GlcNAc-TI	588:596	arg1	gly-14					624:629	gly-14	624:629	gly-14	624:629	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	41	theme	GlcNAc-TI	588:596	arg1	gly-13					613:618	gly-13	613:618	gly-13	613:618	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	41	theme	GlcNAc-TI	588:596	arg1	gly-12					605:610	gly-12	605:610	gly-12	605:610	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	41	theme	GlcNAc-TI	588:596	arg1	genes					598:602	three different GlcNAc-TI genes	572:602	three different GlcNAc-TI genes (gly-12, gly-13 and gly-14)	572:630	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	2	42	theme	paucimannosidic	450:464	arg1	forms					466:470	either complex or paucimannosidic forms	432:470	either complex or paucimannosidic forms	432:470	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	3	43	contain	has	568:570	arg2	gly-13					613:618	gly-13	613:618	gly-13	613:618	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	43	contain	has	568:570	arg2	gly-12					605:610	gly-12	605:610	gly-12	605:610	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	43	contain	has	568:570	arg2	genes					598:602	three different GlcNAc-TI genes	572:602	three different GlcNAc-TI genes (gly-12, gly-13 and gly-14)	572:630	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	43	contain	has	568:570	arg2	gly-14					624:629	gly-14	624:629	gly-14	624:629	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	3	43	contain	has	568:570	arg1	organism					559:566	this organism	554:566	this organism	554:566	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	5	44	from	galactoses	1077:1086	arg1	forms					1174:1178	isomeric forms	1165:1178	isomeric forms of Hex4-8HexNAc2 structures	1165:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	44	from	galactoses	1077:1086	arg1	positions					1107:1115	three different positions	1091:1115	three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures	1091:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	3	45	theme	strain	733:738	arg1	generation					700:709	the generation	696:709	the generation of a triple knock-out strain	696:738	Unusually, this organism has three different GlcNAc-TI genes (gly-12, gly-13 and gly-14); therefore, a complete abolition of GlcNAc-TI activity required the generation of a triple knock-out strain.					
29981898	5	46	theme	gly-12	1008:1013	arg1	N-glycans					991:999	the N-glycans	987:999	the N-glycans of the gly-12;gly-13;gly-14 triple mutant	987:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	2	47	located	found	491:495	arg1	elegans					534:540	the model nematode Caenorhabditis elegans	500:540	the model nematode Caenorhabditis elegans	500:540	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	2	47	located	found	491:495	arg2	both					473:476	both	473:476	both	473:476	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	2	47	located	found	491:495	arg2	forms					466:470	either complex or paucimannosidic forms	432:470	either complex or paucimannosidic forms	432:470	The enzyme is key for the normal processing of N-glycans to either complex or paucimannosidic forms, both of which are found in the model nematode Caenorhabditis elegans.					
29981898	6	48	theme	Golgi	1451:1455	arg1	apparatus					1457:1465	the Golgi apparatus	1447:1465	the Golgi apparatus	1447:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	5	49	theme	hexose	968:973	arg1	residues					975:982	the multiple hexose residues	955:982	the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant	955:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	49	theme	hexose	968:973	arg1	N-glycans					991:999	the N-glycans	987:999	the N-glycans of the gly-12;gly-13;gly-14 triple mutant	987:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	49	theme	hexose	968:973	arg1	mannose					1056:1062	mannose	1056:1062	mannose	1056:1062	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	50	theme	exoglycosidase	903:916	arg1	digestions					918:927	exoglycosidase digestions	903:927	exoglycosidase digestions	903:927	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	1	51	from	modification	133:144	arg1	Golgi					153:157	the Golgi	149:157	the Golgi of N-glycans	149:170	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	6	52	theme	N-glycomic	1279:1288	arg1	wider					1327:1331	wider	1327:1331	wider	1327:1331	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	52	theme	N-glycomic	1279:1288	arg1	repertoire					1290:1299	the N-glycomic repertoire	1275:1299	the N-glycomic repertoire of Caenorhabditis	1275:1317	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	6	52	theme	N-glycomic	1279:1288	arg1	Caenorhabditis					1304:1317	Caenorhabditis	1304:1317	Caenorhabditis	1304:1317	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	5	53	theme	multiple	959:966	arg1	residues					975:982	the multiple hexose residues	955:982	the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant	955:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	53	theme	multiple	959:966	arg1	N-glycans					991:999	the N-glycans	987:999	the N-glycans of the gly-12;gly-13;gly-14 triple mutant	987:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	53	theme	multiple	959:966	arg1	mannose					1056:1062	mannose	1056:1062	mannose	1056:1062	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	5	54	theme	Hex4-8HexNAc2	1183:1195	arg1	structures					1197:1206	Hex4-8HexNAc2 structures	1183:1206	Hex4-8HexNAc2 structures	1183:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	0	55	theme	intersected	93:103	arg1	N-glycans					118:126	unusual intersected and bisected N-glycans	85:126	unusual intersected and bisected N-glycans	85:126	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.					
29981898	5	56	theme	gly-14	1022:1027	arg1	mutant					1036:1041	gly-14 triple mutant	1022:1041	the gly-12;gly-13;gly-14 triple mutant	1004:1041	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	4	57	theme	N-glycans	773:781	arg1	compositions					757:768	the compositions	753:768	the compositions of N-glycans from this mutant	753:798	Previously, the compositions of N-glycans from this mutant were described, but no detailed structures.					
29981898	1	58	theme	N-glycans	162:170	arg1	Golgi					153:157	the Golgi	149:157	the Golgi of N-glycans	149:170	The modification in the Golgi of N-glycans by N-acetylglucosaminyltransferase I (GlcNAc-TI, MGAT1) can be considered to be a hallmark of multicellular eukaryotes as it is found in all metazoans and plants, but rarely in unicellular organisms.					
29981898	6	59	theme	enzymes	1434:1440	arg1	absence					1401:1407	the absence	1397:1407	the absence of key glycan processing enzymes from the Golgi apparatus	1397:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
29981898	5	60	theme	structures	1197:1206	arg1	forms					1174:1178	isomeric forms	1165:1178	isomeric forms of Hex4-8HexNAc2 structures	1165:1206	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	0	61	theme	unusual	85:91	arg1	N-glycans					118:126	unusual intersected and bisected N-glycans	85:126	unusual intersected and bisected N-glycans	85:126	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.					
29981898	0	62	theme	N-acetylglucosaminyltransferases	12:43	arg1	Ablation					0:7	Ablation	0:7	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis	0:61	Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.					
29981898	5	63	theme	off-line	853:860	arg1	approach					880:887	an off-line HPLC-MALDI-TOF-MS approach	850:887	an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS	850:937	Using an off-line HPLC-MALDI-TOF-MS approach combined with exoglycosidase digestions and MS/MS, we reveal that the multiple hexose residues of the N-glycans of the gly-12;gly-13;gly-14 triple mutant are not just mannose, but include galactoses in three different positions (β-intersecting, β-bisecting and α-terminal) on isomeric forms of Hex4-8HexNAc2 structures; some of these structures are fucosylated and/or methylated.					
29981898	6	64	theme	processing	1423:1432	arg1	enzymes					1434:1440	key glycan processing enzymes	1412:1440	key glycan processing enzymes from the Golgi apparatus	1412:1465	Thus, the N-glycomic repertoire of Caenorhabditis is even wider than expected and exhibits a large degree of plasticity even in the absence of key glycan processing enzymes from the Golgi apparatus.					
31373757	7	0	theme	epithelial-to-mesenchymal	1193:1217	arg1	transition					1219:1228	epithelial-to-mesenchymal transition	1193:1228	epithelial-to-mesenchymal transition	1193:1228	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	4	1	dep	O-GlcNAcylation	625:639	arg1	glycosylations					653:666	glycosylations	653:666	glycosylations	653:666	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	1	2	theme	sedentary	216:224	arg1	lifestyle					226:234	a high sedentary lifestyle	209:234	a high sedentary lifestyle	209:234	Colorectal cancer (CRC) affects both women and men living in societies with a high sedentary lifestyle.					
31373757	7	3	theme	transition	1219:1228	arg1	actor					1184:1188	a major actor	1176:1188	a major actor of epithelial-to-mesenchymal transition	1176:1228	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	3	4	from	increase	480:487	arg1	sialylation					582:592	upregulated sialylation	570:592	upregulated sialylation	570:592	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	4	from	increase	480:487	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	4	from	increase	480:487	arg1	fucosylation					497:508	core fucosylation	492:508	core fucosylation	492:508	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	4	from	increase	480:487	arg1	alteration					545:554	alteration	545:554	alteration of O-glycans	545:567	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	4	from	increase	480:487	arg1	O-GlcNAcylation					599:613	O-GlcNAcylation	599:613	O-GlcNAcylation	599:613	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	4	from	increase	480:487	arg1	branching					521:529	GlcNAc branching	514:529	GlcNAc branching	514:529	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	2	5	theme	glycosylation	310:322	arg1	range					301:305	a wide range	294:305	a wide range of glycosylation	294:322	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	3	6	from	branching	521:529	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	7	7	dep	E-cadherin	1164:1173	arg1	actor					1184:1188	a major actor	1176:1188	a major actor of epithelial-to-mesenchymal transition	1176:1228	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	3	8	theme	core	492:495	arg1	fucosylation					497:508	core fucosylation	492:508	core fucosylation	492:508	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	9	gly	fucosylation	497:508	arg1	O-glycans					559:567	O-glycans	559:567	O-glycans	559:567	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	9	gly	fucosylation	497:508	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	0	10	theme	Glycosphingolipid	77:93	arg1	Structures					95:104	Glycosphingolipid Structures	77:104	Glycosphingolipid Structures in Human Colon Cell Lines	77:130	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	9	11	theme	selective	1471:1479	arg1	regulation					1481:1490	the selective regulation	1467:1490	the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells	1467:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	6	12	theme	O-GlcNAc	974:981	arg1	transferase					983:993	silencing O-GlcNAc transferase	964:993	silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation)	964:1040	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	0	13	theme	Human	109:113	arg1	Lines					126:130	Human Colon Cell Lines	109:130	Human Colon Cell Lines	109:130	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	9	14	theme	glycosylations	1503:1516	arg1	regulation					1481:1490	the selective regulation	1467:1490	the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells	1467:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	3	15	from	fucosylation	497:508	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	2	16	theme	phenotypic	249:258	arg1	changes					260:266	the phenotypic changes	245:266	the phenotypic changes exhibited by tumor cells	245:291	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	4	17	theme	many	678:681	arg1	aspects					683:689	many aspects	678:689	many aspects	678:689	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	2	18	theme	cell	367:370	arg1	lines					372:376	colon cancer-derived cell lines	346:376	colon cancer-derived cell lines	346:376	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	7	19	theme	major	1178:1182	arg1	actor					1184:1188	a major actor	1176:1188	a major actor of epithelial-to-mesenchymal transition	1176:1228	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	9	20	from	O-GlcNAcylation	1521:1535	arg1	cells					1553:1557	colon cancer cells	1540:1557	colon cancer cells	1540:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	3	21	theme	GlcNAc	514:519	arg1	branching					521:529	GlcNAc branching	514:529	GlcNAc branching	514:529	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	22	from	O-GlcNAcylation	599:613	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	2	23	theme	cancer-derived	352:365	arg1	lines					372:376	colon cancer-derived cell lines	346:376	colon cancer-derived cell lines	346:376	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	6	24	theme	cell	896:899	arg1	HCT116					914:919	HCT116	914:919	HCT116	914:919	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	24	theme	cell	896:899	arg1	HT29					908:911	HT29	908:911	HT29	908:911	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	24	theme	cell	896:899	arg1	lines					901:905	three human colon cell lines	878:905	three human colon cell lines (HT29, HCT116 and CCD841CoN)	878:934	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	24	theme	cell	896:899	arg1	CCD841CoN					925:933	CCD841CoN	925:933	CCD841CoN	925:933	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	25	theme	overall	1065:1071	arg1	patterns					1096:1103	overall N- and O-glycosylation patterns	1065:1103	overall N- and O-glycosylation patterns	1065:1103	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	4	26	theme	O-GlcNAcylation	739:753	arg1	interference					723:734	the interference	719:734	the interference of O-GlcNAcylation with complex glycosylation	719:780	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	6	27	theme	N-	1073:1074	arg1	patterns					1096:1103	overall N- and O-glycosylation patterns	1065:1103	overall N- and O-glycosylation patterns	1065:1103	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	2	28	theme	colon	346:350	arg1	lines					372:376	colon cancer-derived cell lines	346:376	colon cancer-derived cell lines	346:376	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	6	29	theme	silencing	964:972	arg1	transferase					983:993	silencing O-GlcNAc transferase	964:993	silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation)	964:1040	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	0	30	theme	Cell	121:124	arg1	Lines					126:130	Human Colon Cell Lines	109:130	Human Colon Cell Lines	109:130	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	9	31	theme	novel	1442:1446	arg1	insights					1448:1455	novel insights	1442:1455	novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells	1442:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	2	32	theme	tumor	281:285	arg1	cells					287:291	tumor cells	281:291	tumor cells	281:291	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	3	33	gly	sialylation	582:592	arg1	O-glycans					559:567	O-glycans	559:567	O-glycans	559:567	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	33	gly	sialylation	582:592	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	5	34	theme	debate	836:841	arg1	matter					826:831	a matter	824:831	a matter of debate	824:841	Nevertheless, this relationship is still a matter of debate.					
31373757	5	34	theme	debate	836:841	arg1	relationship					802:813	this relationship	797:813	this relationship	797:813	Nevertheless, this relationship is still a matter of debate.					
31373757	0	35	theme	Colon	115:119	arg1	Lines					126:130	Human Colon Cell Lines	109:130	Human Colon Cell Lines	109:130	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	6	36	theme	colon	890:894	arg1	HCT116					914:919	HCT116	914:919	HCT116	914:919	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	36	theme	colon	890:894	arg1	HT29					908:911	HT29	908:911	HT29	908:911	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	36	theme	colon	890:894	arg1	lines					901:905	three human colon cell lines	878:905	three human colon cell lines (HT29, HCT116 and CCD841CoN)	878:934	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	36	theme	colon	890:894	arg1	CCD841CoN					925:933	CCD841CoN	925:933	CCD841CoN	925:933	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	4	37	theme	sparse	692:697	arg1	evidences					699:707	sparse evidences	692:707	sparse evidences	692:707	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	2	38	theme	CRC	382:384	arg1	tissues					386:392	CRC tissues	382:392	CRC tissues	382:392	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	8	39	theme	glycosphingolipids	1320:1337	arg1	biosynthesis					1304:1315	biosynthesis	1304:1315	biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides	1304:1407	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	6	40	theme	human	884:888	arg1	HCT116					914:919	HCT116	914:919	HCT116	914:919	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	40	theme	human	884:888	arg1	HT29					908:911	HT29	908:911	HT29	908:911	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	40	theme	human	884:888	arg1	lines					901:905	three human colon cell lines	878:905	three human colon cell lines (HT29, HCT116 and CCD841CoN)	878:934	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	40	theme	human	884:888	arg1	CCD841CoN					925:933	CCD841CoN	925:933	CCD841CoN	925:933	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	3	41	theme	upregulated	570:580	arg1	sialylation					582:592	upregulated sialylation	570:592	upregulated sialylation	570:592	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	42	from	sialylation	582:592	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	43	theme	aberrant	401:408	arg1	modifications					410:422	These aberrant modifications	395:422	These aberrant modifications	395:422	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	7	44	theme	HT29	1141:1144	arg1	cells					1146:1150	HT29 cells	1141:1150	HT29 cells	1141:1150	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	9	45	theme	complex	1495:1501	arg1	glycosylations					1503:1516	complex glycosylations	1495:1516	complex glycosylations	1495:1516	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	3	46	theme	different	431:439	arg1	aspects					441:447	different aspects	431:447	different aspects	431:447	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	46	theme	different	431:439	arg1	increase					480:487	an increase	477:487	an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation	477:613	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	2	47	link	cancer-derived	352:365	arg1	lines					372:376	colon cancer-derived cell lines	346:376	colon cancer-derived cell lines	346:376	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	3	48	theme	O-glycans	559:567	arg1	branching					521:529	GlcNAc branching	514:529	GlcNAc branching	514:529	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	48	theme	O-glycans	559:567	arg1	alteration					545:554	alteration	545:554	alteration of O-glycans	545:567	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	48	theme	O-glycans	559:567	arg1	sialylation					582:592	upregulated sialylation	570:592	upregulated sialylation	570:592	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	48	theme	O-glycans	559:567	arg1	O-GlcNAcylation					599:613	O-GlcNAcylation	599:613	O-GlcNAcylation	599:613	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	48	theme	O-glycans	559:567	arg1	fucosylation					497:508	core fucosylation	492:508	core fucosylation	492:508	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	8	49	theme	OGT	1281:1283	arg1	silencing					1285:1293	OGT silencing	1281:1293	OGT silencing	1281:1293	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	7	50	theme	OGT	1134:1136	arg1	silencing					1121:1129	silencing	1121:1129	silencing of OGT in HT29 cells	1121:1150	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	6	51	dep	transferase	983:993	arg1	OGT					996:998	OGT	996:998	OGT	996:998	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	51	dep	transferase	983:993	arg1	enzyme					1010:1015	the sole enzyme	1001:1015	the sole enzyme driving O-GlcNAcylation	1001:1039	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	3	52	from	N-glycans	534:542	arg1	increase					480:487	an increase	477:487	an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation	477:613	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	7	53	from	silencing	1121:1129	arg1	cells					1146:1150	HT29 cells	1141:1150	HT29 cells	1141:1150	Interestingly, silencing of OGT in HT29 cells upregulates E-cadherin (a major actor of epithelial-to-mesenchymal transition) and changes its glycosylation.					
31373757	6	54	theme	different	854:862	arg1	approaches					864:873	different approaches	854:873	different approaches	854:873	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	55	theme	sole	1005:1008	arg1	OGT					996:998	OGT	996:998	OGT	996:998	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	55	theme	sole	1005:1008	arg1	enzyme					1010:1015	the sole enzyme	1001:1015	the sole enzyme driving O-GlcNAcylation	1001:1039	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	2	56	theme	wide	296:299	arg1	range					301:305	a wide range	294:305	a wide range of glycosylation	294:322	Amongst the phenotypic changes exhibited by tumor cells, a wide range of glycosylation has been reported for colon cancer-derived cell lines and CRC tissues.					
31373757	4	57	theme	complex	760:766	arg1	glycosylation					768:780	complex glycosylation	760:780	complex glycosylation	760:780	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	6	58	dep	lines	901:905	arg1	HCT116					914:919	HCT116	914:919	HCT116	914:919	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	58	dep	lines	901:905	arg1	HT29					908:911	HT29	908:911	HT29	908:911	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	58	dep	lines	901:905	arg1	lines					901:905	three human colon cell lines	878:905	three human colon cell lines (HT29, HCT116 and CCD841CoN)	878:934	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	6	58	dep	lines	901:905	arg1	CCD841CoN					925:933	CCD841CoN	925:933	CCD841CoN	925:933	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	0	59	from	Structures	95:104	arg1	Lines					126:130	Human Colon Cell Lines	109:130	Human Colon Cell Lines	109:130	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	9	60	theme	cancer	1546:1551	arg1	cells					1553:1557	colon cancer cells	1540:1557	colon cancer cells	1540:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	3	61	from	alteration	545:554	arg1	N-glycans					534:542	N-glycans	534:542	N-glycans	534:542	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	62	theme	glycosylation	452:464	arg1	aspects					441:447	different aspects	431:447	different aspects	431:447	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	3	62	theme	glycosylation	452:464	arg1	increase					480:487	an increase	477:487	an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation	477:613	These aberrant modifications affect different aspects of glycosylation, including an increase in core fucosylation and GlcNAc branching on N-glycans, alteration of O-glycans, upregulated sialylation, and O-GlcNAcylation.					
31373757	4	63	gly	glycosylation	768:780	arg1	interference					723:734	the interference	719:734	the interference of O-GlcNAcylation with complex glycosylation	719:780	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	8	64	theme	other	1269:1273	arg1	hand					1275:1278	the other hand	1265:1278	the other hand	1265:1278	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	0	65	gly	Glycosylation	36:48	arg1	E-cadherin					53:62	E-cadherin	53:62	E-cadherin	53:62	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	9	66	theme	colon	1540:1544	arg1	cells					1553:1557	colon cancer cells	1540:1557	colon cancer cells	1540:1557	Together, these results provide novel insights regarding the selective regulation of complex glycosylations by O-GlcNAcylation in colon cancer cells.					
31373757	1	67	theme	Colorectal	133:142	arg1	cancer					144:149	Colorectal cancer	133:149	Colorectal cancer (CRC)	133:155	Colorectal cancer (CRC) affects both women and men living in societies with a high sedentary lifestyle.					
31373757	1	67	theme	Colorectal	133:142	arg1	CRC					152:154	CRC	152:154	CRC	152:154	Colorectal cancer (CRC) affects both women and men living in societies with a high sedentary lifestyle.					
31373757	8	68	from	decrease	1354:1361	arg1	globosides					1398:1407	globosides	1398:1407	globosides	1398:1407	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	8	68	from	decrease	1354:1361	arg1	gangliosides					1366:1377	gangliosides	1366:1377	gangliosides	1366:1377	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	0	69	theme	E-cadherin	53:62	arg1	Expression					17:26	the Expression	13:26	the Expression	13:26	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	0	69	theme	E-cadherin	53:62	arg1	Glycosylation					36:48	the Glycosylation	32:48	the Glycosylation of E-cadherin	32:62	OGT Controls the Expression and the Glycosylation of E-cadherin, and Affects Glycosphingolipid Structures in Human Colon Cell Lines.					
31373757	4	70	with	interference	723:734	arg1	glycosylation					768:780	complex glycosylation	760:780	complex glycosylation	760:780	Although O-GlcNAcylation and complex glycosylations differ in many aspects, sparse evidences report on the interference of O-GlcNAcylation with complex glycosylation.					
31373757	1	71	with	societies	194:202	arg1	lifestyle					226:234	a high sedentary lifestyle	209:234	a high sedentary lifestyle	209:234	Colorectal cancer (CRC) affects both women and men living in societies with a high sedentary lifestyle.					
31373757	6	72	theme	O-glycosylation	1080:1094	arg1	patterns					1096:1103	overall N- and O-glycosylation patterns	1065:1103	overall N- and O-glycosylation patterns	1065:1103	Combining different approaches on three human colon cell lines (HT29, HCT116 and CCD841CoN), it is herein reported that silencing O-GlcNAc transferase (OGT, the sole enzyme driving O-GlcNAcylation), only slightly affects overall N- and O-glycosylation patterns.					
31373757	8	73	from	increase	1386:1393	arg1	globosides					1398:1407	globosides	1398:1407	globosides	1398:1407	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	8	73	from	increase	1386:1393	arg1	gangliosides					1366:1377	gangliosides	1366:1377	gangliosides	1366:1377	On the other hand, OGT silencing perturbs biosynthesis of glycosphingolipids resulting in a decrease in gangliosides and an increase in globosides.					
31373757	1	74	theme	high	211:214	arg1	lifestyle					226:234	a high sedentary lifestyle	209:234	a high sedentary lifestyle	209:234	Colorectal cancer (CRC) affects both women and men living in societies with a high sedentary lifestyle.					
31669586	8	0	with	increase	896:903	arg1	FA2G2S2					1017:1023	FA2G2S2	1017:1023	FA2G2S2	1017:1023	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	0	with	increase	896:903	arg1	fucose					1009:1014	antennary fucose	999:1014	antennary fucose (FA2G2S2)	999:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	11	1	theme	lectin	1368:1373	arg1	reaction					1384:1391	Maackia amurensis II lectin (MAL-II) reaction	1347:1391	Maackia amurensis II lectin (MAL-II) reaction	1347:1391	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	11	2	gly	α2,3-sialylation	1428:1443	arg1	sera					1451:1454	HT sera	1448:1454	HT sera	1448:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	8	3	theme	antennary	999:1007	arg1	FA2G2S2					1017:1023	FA2G2S2	1017:1023	FA2G2S2	1017:1023	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	3	theme	antennary	999:1007	arg1	fucose					1009:1014	antennary fucose	999:1014	antennary fucose (FA2G2S2)	999:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	13	4	theme	obtained	1574:1581	arg1	results					1583:1589	The obtained results	1570:1589	The obtained results	1570:1589	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	6	5	theme	collected	678:686	arg1	fraction					693:700	each collected HPLC fraction	673:700	each collected HPLC fraction	673:700	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	11	6	theme	reaction	1384:1391	arg1	increase					1335:1342	an increase	1332:1342	an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera	1332:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	5	7	theme	high-performance	600:615	arg1	NP-HPLC					640:646	NP-HPLC	640:646	NP-HPLC	640:646	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	7	theme	high-performance	600:615	arg1	chromatography					624:637	normal-phase high-performance liquid chromatography	587:637	normal-phase high-performance liquid chromatography (NP-HPLC)	587:647	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	1	8	theme	chronic	166:172	arg1	inflammation					174:185	chronic inflammation	166:185	chronic inflammation of thyroid gland	166:202	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	6	9	from	structures	659:668	arg1	fraction					693:700	each collected HPLC fraction	673:700	each collected HPLC fraction	673:700	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	13	10	theme	autoimmunity	1755:1766	arg1	process					1768:1774	autoimmunity process	1755:1774	autoimmunity process in HT	1755:1780	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	6	11	theme	exoglycosidase	773:786	arg1	digestion					788:796	exoglycosidase digestion	773:796	exoglycosidase digestion	773:796	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	4	12	gly	glycosylation	436:448	arg1	sera					474:477	IgG-depleted sera	461:477	IgG-depleted sera from HT patients	461:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	10	13	from	reduction	1265:1273	arg1	serum					1308:1312	HT serum	1305:1312	HT serum	1305:1312	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	1	14	theme	gland	198:202	arg1	inflammation					174:185	chronic inflammation	166:185	chronic inflammation of thyroid gland	166:202	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	11	15	theme	amurensis	1355:1363	arg1	MAL-II					1376:1381	MAL-II	1376:1381	MAL-II	1376:1381	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	11	15	theme	amurensis	1355:1363	arg1	lectin					1368:1373	Maackia amurensis II lectin	1347:1373	Maackia amurensis II lectin (MAL-II) reaction	1347:1391	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	10	16	theme	α1,6-linked	1278:1288	arg1	fucose					1295:1300	α1,6-linked core fucose	1278:1300	α1,6-linked core fucose	1278:1300	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	5	17	theme	N-glycosidase	533:545	arg1	F					557:557	PNGase F	550:557	PNGase F	550:557	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	17	theme	N-glycosidase	533:545	arg1	F					547:547	N-glycosidase F	533:547	N-glycosidase F (PNGase F) digestion	533:568	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	12	18	dep	CONCLUSIONS	1457:1467	arg1	caused					1532:1537	caused	1532:1537	might be caused by chronic inflammation in HT	1523:1567	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	14	19	theme	protein	1860:1866	arg1	sialylation					1868:1878	serum protein sialylation	1854:1878	serum protein sialylation	1854:1878	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	10	20	theme	HT	1229:1230	arg1	samples					1232:1238	HT samples	1229:1238	HT samples	1229:1238	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	11	21	from	increase	1335:1342	arg1	HT					1396:1397	HT	1396:1397	HT due to the elevated level of α2,3-sialylation in HT sera	1396:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	2	22	theme	common	229:234	arg1	cause					236:240	the most common cause	220:240	the most common cause of hypothyroidism	220:258	Although HT is the most common cause of hypothyroidism, the pathogenesis of this disease is not fully understood.					
31669586	2	22	theme	common	229:234	arg1	HT					214:215	HT	214:215	HT	214:215	Although HT is the most common cause of hypothyroidism, the pathogenesis of this disease is not fully understood.					
31669586	5	23	theme	Serum	505:509	arg1	N-glycans					511:519	METHODS Serum N-glycans	497:519	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion	497:568	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	1	24	theme	autoimmune	130:139	arg1	disease					141:147	an autoimmune disease	127:147	an autoimmune disease characterized by chronic inflammation of thyroid gland	127:202	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	1	24	theme	autoimmune	130:139	arg1	thyroiditis					107:117	BACKGROUND Hashimoto's thyroiditis	84:117	BACKGROUND Hashimoto's thyroiditis (HT)	84:122	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	0	25	from	profile	17:23	arg1	thyroiditis					71:81	Hashimoto's thyroiditis	59:81	Hashimoto's thyroiditis	59:81	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	12	26	theme	serum	1497:1501	arg1	sialylation					1511:1521	serum protein sialylation	1497:1521	serum protein sialylation	1497:1521	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	3	27	theme	proteins	342:349	arg1	Glycosylation					319:331	Glycosylation	319:331	Glycosylation of serum proteins	319:349	Glycosylation of serum proteins was examined in HT only to a limited extent.					
31669586	4	28	theme	HT	484:485	arg1	patients					487:494	HT patients	484:494	HT patients	484:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	9	29	theme	lectin	1083:1088	arg1	blotting					1090:1097	lectin blotting	1083:1097	lectin blotting using lectins specific for fucose and sialic acid	1083:1147	Subsequently, we analyzed the serum N-glycan profile by lectin blotting using lectins specific for fucose and sialic acid.					
31669586	13	30	from	process	1768:1774	arg1	HT					1779:1780	HT	1779:1780	HT	1779:1780	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	12	31	theme	sialylation	1511:1521	arg1	alterations					1482:1492	The detected alterations	1469:1492	The detected alterations of serum protein sialylation	1469:1521	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	4	32	theme	glycosylation	436:448	arg1	pattern					450:456	the glycosylation pattern	432:456	the glycosylation pattern of IgG-depleted sera from HT patients	432:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	10	33	theme	core	1290:1293	arg1	fucose					1295:1300	α1,6-linked core fucose	1278:1300	α1,6-linked core fucose	1278:1300	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	14	34	theme	Thyroid	1804:1810	arg1	autoimmunity					1812:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	9	35	theme	specific	1113:1120	arg1	lectins					1105:1111	lectins	1105:1111	lectins specific for fucose and sialic acid	1105:1147	Subsequently, we analyzed the serum N-glycan profile by lectin blotting using lectins specific for fucose and sialic acid.					
31669586	0	36	theme	Altered	0:6	arg1	profile					17:23	Altered N-glycan profile	0:23	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis	0:81	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	11	37	from	sera	1451:1454	arg1	level					1419:1423	the elevated level	1406:1423	the elevated level of α2,3-sialylation in HT sera	1406:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	8	38	theme	monosialylated	908:921	arg1	A3G3S1					948:953	A3G3S1	948:953	A3G3S1	948:953	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	38	theme	monosialylated	908:921	arg1	structure					937:945	monosialylated tri-antennary structure	908:945	monosialylated tri-antennary structure (A3G3S1)	908:954	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	10	39	link	α1,6-linked	1278:1288	arg1	fucose					1295:1300	α1,6-linked core fucose	1278:1300	α1,6-linked core fucose	1278:1300	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	12	40	theme	detected	1473:1480	arg1	alterations					1482:1492	The detected alterations	1469:1492	The detected alterations of serum protein sialylation	1469:1521	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	6	41	theme	N-glycan	650:657	arg1	structures					659:668	N-glycan structures	650:668	N-glycan structures in each collected HPLC fraction	650:700	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	8	42	theme	structure	937:945	arg1	increase					896:903	an increase	893:903	an increase of monosialylated tri-antennary structure (A3G3S1)	893:954	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	42	theme	structure	937:945	arg1	N-glycan					985:992	disialylated diantennary N-glycan	960:992	disialylated diantennary N-glycan with antennary fucose (FA2G2S2)	960:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	3	43	theme	limited	380:386	arg1	extent					388:393	a limited extent	378:393	a limited extent	378:393	Glycosylation of serum proteins was examined in HT only to a limited extent.					
31669586	13	44	theme	autoimmune	1645:1654	arg1	patients					1664:1671	autoimmune thyroid patients	1645:1671	autoimmune thyroid patients	1645:1671	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	9	45	theme	sialic	1137:1142	arg1	acid					1144:1147	sialic acid	1137:1147	sialic acid	1137:1147	Subsequently, we analyzed the serum N-glycan profile by lectin blotting using lectins specific for fucose and sialic acid.					
31669586	0	46	theme	IgG-depleted	28:39	arg1	proteins					47:54	IgG-depleted serum proteins	28:54	IgG-depleted serum proteins	28:54	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	13	47	theme	proteins	1724:1731	arg1	characteristic					1736:1749	characteristic	1736:1749	characteristic	1736:1749	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	13	47	theme	proteins	1724:1731	arg1	N-glycosylation					1699:1713	the altered N-glycosylation	1687:1713	the altered N-glycosylation of serum proteins	1687:1731	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	14	48	theme	Significance	1791:1802	arg1	autoimmunity					1812:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	8	49	dep	RESULTS	866:872	arg1	showed					886:891	showed	886:891	showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2)	886:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	0	50	theme	proteins	47:54	arg1	profile					17:23	Altered N-glycan profile	0:23	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis	0:81	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	10	51	theme	significant	1161:1171	arg1	decrease					1173:1180	a significant decrease	1159:1180	a significant decrease	1159:1180	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	13	52	theme	IgG	1613:1615	arg1	analysis					1633:1640	our previous IgG N-glycosylation analysis	1600:1640	our previous IgG N-glycosylation analysis	1600:1640	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	12	53	from	inflammation	1550:1561	arg1	HT					1566:1567	HT	1566:1567	HT	1566:1567	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	8	54	theme	diantennary	973:983	arg1	N-glycan					985:992	disialylated diantennary N-glycan	960:992	disialylated diantennary N-glycan with antennary fucose (FA2G2S2)	960:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	6	55	theme	liquid	721:726	arg1	LC-MS					762:766	LC-MS	762:766	LC-MS	762:766	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	6	55	theme	liquid	721:726	arg1	spectrometry					748:759	liquid chromatography-mass spectrometry	721:759	liquid chromatography-mass spectrometry (LC-MS)	721:767	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	10	56	theme	culinaris	1190:1198	arg1	agglutinin					1200:1209	Lens culinaris agglutinin	1185:1209	Lens culinaris agglutinin (LCA) staining in HT samples	1185:1238	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	10	56	theme	culinaris	1190:1198	arg1	LCA					1212:1214	LCA	1212:1214	LCA	1212:1214	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	4	57	from	patients	487:494	arg1	pattern					450:456	the glycosylation pattern	432:456	the glycosylation pattern of IgG-depleted sera from HT patients	432:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	4	57	from	patients	487:494	arg1	sera					474:477	IgG-depleted sera	461:477	IgG-depleted sera from HT patients	461:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	4	58	theme	sera	474:477	arg1	pattern					450:456	the glycosylation pattern	432:456	the glycosylation pattern of IgG-depleted sera from HT patients	432:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	4	59	from	pattern	450:456	arg1	patients					487:494	HT patients	484:494	HT patients	484:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	8	60	gly	disialylated	960:971	arg1	N-glycan					985:992	disialylated diantennary N-glycan	960:992	disialylated diantennary N-glycan with antennary fucose (FA2G2S2)	960:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	5	61	theme	normal-phase	587:598	arg1	NP-HPLC					640:646	NP-HPLC	640:646	NP-HPLC	640:646	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	61	theme	normal-phase	587:598	arg1	chromatography					624:637	normal-phase high-performance liquid chromatography	587:637	normal-phase high-performance liquid chromatography (NP-HPLC)	587:647	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	62	theme	liquid	617:622	arg1	NP-HPLC					640:646	NP-HPLC	640:646	NP-HPLC	640:646	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	62	theme	liquid	617:622	arg1	chromatography					624:637	normal-phase high-performance liquid chromatography	587:637	normal-phase high-performance liquid chromatography (NP-HPLC)	587:647	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	13	63	theme	previous	1604:1611	arg1	analysis					1633:1640	our previous IgG N-glycosylation analysis	1600:1640	our previous IgG N-glycosylation analysis	1600:1640	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	2	64	theme	disease	286:292	arg1	pathogenesis					265:276	the pathogenesis	261:276	the pathogenesis of this disease	261:292	Although HT is the most common cause of hypothyroidism, the pathogenesis of this disease is not fully understood.					
31669586	7	65	theme	lectin	849:854	arg1	blotting					856:863	lectin blotting	849:863	lectin blotting	849:863	Fucosylation and sialylation was also analyzed by lectin blotting.					
31669586	1	66	theme	thyroid	190:196	arg1	gland					198:202	thyroid gland	190:202	thyroid gland	190:202	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	11	67	theme	Maackia	1347:1353	arg1	MAL-II					1376:1381	MAL-II	1376:1381	MAL-II	1376:1381	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	11	67	theme	Maackia	1347:1353	arg1	lectin					1368:1373	Maackia amurensis II lectin	1347:1373	Maackia amurensis II lectin (MAL-II) reaction	1347:1391	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	2	68	theme	hypothyroidism	245:258	arg1	cause					236:240	the most common cause	220:240	the most common cause of hypothyroidism	220:258	Although HT is the most common cause of hypothyroidism, the pathogenesis of this disease is not fully understood.					
31669586	2	68	theme	hypothyroidism	245:258	arg1	HT					214:215	HT	214:215	HT	214:215	Although HT is the most common cause of hypothyroidism, the pathogenesis of this disease is not fully understood.					
31669586	5	69	theme	F	547:547	arg1	digestion					560:568	N-glycosidase F (PNGase F) digestion	533:568	N-glycosidase F (PNGase F) digestion	533:568	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	14	70	theme	sialylation	1868:1878	arg1	changes					1843:1849	changes	1843:1849	changes of serum protein sialylation	1843:1878	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	11	71	theme	HT	1448:1449	arg1	sera					1451:1454	HT sera	1448:1454	HT sera	1448:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	5	72	theme	METHODS	497:503	arg1	N-glycans					511:519	METHODS Serum N-glycans	497:519	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion	497:568	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	14	73	theme	serum	1854:1858	arg1	sialylation					1868:1878	serum protein sialylation	1854:1878	serum protein sialylation	1854:1878	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	13	74	gly	N-glycosylation	1699:1713	arg1	proteins					1724:1731	serum proteins	1718:1731	serum proteins	1718:1731	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	12	75	theme	chronic	1542:1548	arg1	inflammation					1550:1561	chronic inflammation	1542:1561	chronic inflammation in HT	1542:1567	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	9	76	theme	serum	1057:1061	arg1	profile					1072:1078	the serum N-glycan profile	1053:1078	the serum N-glycan profile	1053:1078	Subsequently, we analyzed the serum N-glycan profile by lectin blotting using lectins specific for fucose and sialic acid.					
31669586	9	77	theme	N-glycan	1063:1070	arg1	profile					1072:1078	the serum N-glycan profile	1053:1078	the serum N-glycan profile	1053:1078	Subsequently, we analyzed the serum N-glycan profile by lectin blotting using lectins specific for fucose and sialic acid.					
31669586	12	78	theme	protein	1503:1509	arg1	sialylation					1511:1521	serum protein sialylation	1497:1521	serum protein sialylation	1497:1521	CONCLUSIONS The detected alterations of serum protein sialylation might be caused by chronic inflammation in HT.					
31669586	13	79	theme	altered	1691:1697	arg1	characteristic					1736:1749	characteristic	1736:1749	characteristic	1736:1749	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	13	79	theme	altered	1691:1697	arg1	N-glycosylation					1699:1713	the altered N-glycosylation	1687:1713	the altered N-glycosylation of serum proteins	1687:1731	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	10	80	theme	fucose	1295:1300	arg1	reduction					1265:1273	the reduction	1261:1273	the reduction of α1,6-linked core fucose in HT serum	1261:1312	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	3	81	theme	serum	336:340	arg1	proteins					342:349	serum proteins	336:349	serum proteins	336:349	Glycosylation of serum proteins was examined in HT only to a limited extent.					
31669586	4	82	theme	IgG-depleted	461:472	arg1	sera					474:477	IgG-depleted sera	461:477	IgG-depleted sera from HT patients	461:494	The study was designed to determine the glycosylation pattern of IgG-depleted sera from HT patients.					
31669586	13	83	theme	serum	1718:1722	arg1	proteins					1724:1731	serum proteins	1718:1731	serum proteins	1718:1731	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	10	84	theme	agglutinin	1200:1209	arg1	decrease					1173:1180	a significant decrease	1159:1180	a significant decrease	1159:1180	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	6	85	theme	HPLC	688:691	arg1	fraction					693:700	each collected HPLC fraction	673:700	each collected HPLC fraction	673:700	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	0	86	theme	N-glycan	8:15	arg1	profile					17:23	Altered N-glycan profile	0:23	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis	0:81	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	5	87	theme	PNGase	550:555	arg1	F					557:557	PNGase F	550:557	PNGase F	550:557	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	5	87	theme	PNGase	550:555	arg1	F					547:547	N-glycosidase F	533:547	N-glycosidase F (PNGase F) digestion	533:568	METHODS Serum N-glycans released by N-glycosidase F (PNGase F) digestion were analyzed by normal-phase high-performance liquid chromatography (NP-HPLC).					
31669586	1	88	theme	BACKGROUND	84:93	arg1	Hashimoto					95:103	BACKGROUND Hashimoto's	84:105	BACKGROUND Hashimoto's thyroiditis (HT)	84:122	BACKGROUND Hashimoto's thyroiditis (HT) is an autoimmune disease characterized by chronic inflammation of thyroid gland.					
31669586	11	89	theme	α2,3-sialylation	1428:1443	arg1	level					1419:1423	the elevated level	1406:1423	the elevated level of α2,3-sialylation in HT sera	1406:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	10	90	theme	HT	1305:1306	arg1	serum					1308:1312	HT serum	1305:1312	HT serum	1305:1312	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	0	91	theme	serum	41:45	arg1	proteins					47:54	IgG-depleted serum proteins	28:54	IgG-depleted serum proteins	28:54	Altered N-glycan profile of IgG-depleted serum proteins in Hashimoto's thyroiditis.					
31669586	11	92	theme	elevated	1410:1417	arg1	level					1419:1423	the elevated level	1406:1423	the elevated level of α2,3-sialylation in HT sera	1406:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	11	93	from	level	1419:1423	arg1	sera					1451:1454	HT sera	1448:1454	HT sera	1448:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	8	94	theme	tri-antennary	923:935	arg1	A3G3S1					948:953	A3G3S1	948:953	A3G3S1	948:953	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	94	theme	tri-antennary	923:935	arg1	structure					937:945	monosialylated tri-antennary structure	908:945	monosialylated tri-antennary structure (A3G3S1)	908:954	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	95	with	N-glycan	985:992	arg1	FA2G2S2					1017:1023	FA2G2S2	1017:1023	FA2G2S2	1017:1023	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	95	with	N-glycan	985:992	arg1	fucose					1009:1014	antennary fucose	999:1014	antennary fucose (FA2G2S2)	999:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	13	96	theme	thyroid	1656:1662	arg1	patients					1664:1671	autoimmune thyroid patients	1645:1671	autoimmune thyroid patients	1645:1671	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	14	97	theme	General	1783:1789	arg1	autoimmunity					1812:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity	1783:1823	General Significance Thyroid autoimmunity is accompanied by changes of serum protein sialylation.					
31669586	3	98	gly	Glycosylation	319:331	arg1	HT					367:368	HT	367:368	HT	367:368	Glycosylation of serum proteins was examined in HT only to a limited extent.					
31669586	3	98	gly	Glycosylation	319:331	arg1	proteins					342:349	serum proteins	336:349	serum proteins	336:349	Glycosylation of serum proteins was examined in HT only to a limited extent.					
31669586	11	99	from	α2,3-sialylation	1428:1443	arg1	sera					1451:1454	HT sera	1448:1454	HT sera	1448:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	8	100	gly	monosialylated	908:921	arg1	A3G3S1					948:953	A3G3S1	948:953	A3G3S1	948:953	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	8	100	gly	monosialylated	908:921	arg1	structure					937:945	monosialylated tri-antennary structure	908:945	monosialylated tri-antennary structure (A3G3S1)	908:954	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	13	101	theme	N-glycosylation	1617:1631	arg1	analysis					1633:1640	our previous IgG N-glycosylation analysis	1600:1640	our previous IgG N-glycosylation analysis	1600:1640	The obtained results complete our previous IgG N-glycosylation analysis in autoimmune thyroid patients and show that the altered N-glycosylation of serum proteins is characteristic for autoimmunity process in HT.					
31669586	6	102	theme	chromatography-mass	728:746	arg1	LC-MS					762:766	LC-MS	762:766	LC-MS	762:766	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	6	102	theme	chromatography-mass	728:746	arg1	spectrometry					748:759	liquid chromatography-mass spectrometry	721:759	liquid chromatography-mass spectrometry (LC-MS)	721:767	N-glycan structures in each collected HPLC fraction were determined by liquid chromatography-mass spectrometry (LC-MS) and exoglycosidase digestion.					
31669586	11	103	theme	due	1399:1401	arg1	HT					1396:1397	HT	1396:1397	HT due to the elevated level of α2,3-sialylation in HT sera	1396:1454	We also observed an increase of Maackia amurensis II lectin (MAL-II) reaction in HT due to the elevated level of α2,3-sialylation in HT sera.					
31669586	8	104	theme	disialylated	960:971	arg1	N-glycan					985:992	disialylated diantennary N-glycan	960:992	disialylated diantennary N-glycan with antennary fucose (FA2G2S2)	960:1024	RESULTS The results showed an increase of monosialylated tri-antennary structure (A3G3S1) and disialylated diantennary N-glycan with antennary fucose (FA2G2S2).					
31669586	10	105	theme	Lens	1185:1188	arg1	agglutinin					1200:1209	Lens culinaris agglutinin	1185:1209	Lens culinaris agglutinin (LCA) staining in HT samples	1185:1238	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
31669586	10	105	theme	Lens	1185:1188	arg1	LCA					1212:1214	LCA	1212:1214	LCA	1212:1214	We found a significant decrease of Lens culinaris agglutinin (LCA) staining in HT samples, which resulted from the reduction of α1,6-linked core fucose in HT serum.					
29981899	5	0	theme	low	662:664	arg1	CLBP					677:680	CLBP	677:680	CLBP	677:680	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	5	0	theme	low	662:664	arg1	pain					671:674	chronic low back pain	654:674	chronic low back pain (CLBP)	654:681	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	6	1	theme	patients	795:802	arg1	samples					774:780	METHODS Plasma samples	759:780	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls	759:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	1	2	theme	patient	237:243	arg1	prognosis					245:253	patient prognosis	237:253	patient prognosis	237:253	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	11	3	from	perspective	1916:1926	arg1	pathology					1949:1957	potential disease pathology	1931:1957	potential disease pathology	1931:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	6	4	from	centers	856:862	arg1	Croatia					886:892	Croatia	886:892	Croatia	886:892	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	4	from	centers	856:862	arg1	Belgium					874:880	Belgium	874:880	Belgium	874:880	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	9	5	theme	disialylated	1471:1482	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	7	6	theme	abdominal	1195:1203	arg1	surgery					1205:1211	abdominal surgery	1195:1211	abdominal surgery	1195:1211	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	9	7	theme	trisialylated	1488:1500	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	10	8	theme	N-glycome	1678:1686	arg1	level					1688:1692	the plasma N-glycome level	1667:1692	the plasma N-glycome level	1667:1692	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	6	9	theme	METHODS	759:765	arg1	samples					774:780	METHODS Plasma samples	759:780	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls	759:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	1	10	theme	underlying	157:166	arg1	mechanisms					168:177	heterogeneous underlying mechanisms	143:177	heterogeneous underlying mechanisms	143:177	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	6	11	theme	interaction	948:958	arg1	HILIC-UPLC					1001:1010	HILIC-UPLC	1001:1010	HILIC-UPLC	1001:1010	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	11	theme	interaction	948:958	arg1	chromatography					985:998	hydrophilic interaction ultra-performance liquid chromatography	936:998	hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC)	936:1011	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	11	12	theme	plasma	1873:1878	arg1	N-glycome					1880:1888	plasma N-glycome	1873:1888	plasma N-glycome	1873:1888	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	7	13	theme	N-glycosylation	1082:1096	arg1	profiles					1098:1105	Observed N-glycosylation profiles	1073:1105	Observed N-glycosylation profiles	1073:1105	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	8	14	theme	high-branched	1299:1311	arg1	structures					1358:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	6	15	theme	N-glycosylation	907:921	arg1	profiling					923:931	N-glycosylation profiling	907:931	N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up	907:1070	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	1	16	dep	BACKGROUND	68:77	arg1	pain					88:91	back pain	83:91	back pain (LBP)	83:97	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	1	16	dep	BACKGROUND	68:77	arg1	symptom					106:112	the symptom	102:112	the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies	102:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	11	17	theme	new	1902:1904	arg1	perspective					1916:1926	a new glycomics perspective	1900:1926	a new glycomics perspective on potential disease pathology	1900:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	8	18	from	increase	1264:1271	arg1	amount					1289:1294	the relative amount	1276:1294	the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins	1276:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	18	from	increase	1264:1271	arg1	structures					1358:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	18	from	increase	1264:1271	arg1	glycoproteins					1394:1406	CLBP patients' plasma glycoproteins	1372:1406	CLBP patients' plasma glycoproteins	1372:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	11	19	theme	clinical	1837:1844	arg1	this					1815:1818	this	1815:1818	this	1815:1818	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	19	theme	clinical	1837:1844	arg1	study					1846:1850	a first large clinical study	1823:1850	a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology	1823:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	6	20	theme	N-glycans	1019:1027	arg1	release					1029:1035	N-glycans release	1019:1035	N-glycans release	1019:1035	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	8	21	theme	N-glycan	1349:1356	arg1	structures					1358:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	11	22	theme	first	1825:1829	arg1	this					1815:1818	this	1815:1818	this	1815:1818	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	22	theme	first	1825:1829	arg1	study					1846:1850	a first large clinical study	1823:1850	a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology	1823:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	8	23	from	amount	1289:1294	arg1	glycoproteins					1394:1406	CLBP patients' plasma glycoproteins	1372:1406	CLBP patients' plasma glycoproteins	1372:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	5	24	theme	disease	750:756	arg1	mechanisms					732:741	the pathogenic mechanisms	717:741	the pathogenic mechanisms of the disease	717:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	5	25	theme	total	602:606	arg1	pattern					631:637	total plasma N-glycosylation pattern	602:637	total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease	602:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	6	26	theme	liquid	978:983	arg1	HILIC-UPLC					1001:1010	HILIC-UPLC	1001:1010	HILIC-UPLC	1001:1010	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	26	theme	liquid	978:983	arg1	chromatography					985:998	hydrophilic interaction ultra-performance liquid chromatography	936:998	hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC)	936:1011	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	8	27	theme	plasma	1387:1392	arg1	glycoproteins					1394:1406	CLBP patients' plasma glycoproteins	1372:1406	CLBP patients' plasma glycoproteins	1372:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	5	28	theme	pathogenic	721:730	arg1	mechanisms					732:741	the pathogenic mechanisms	717:741	the pathogenic mechanisms of the disease	717:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	1	29	theme	Low	79:81	arg1	BACKGROUND					68:77	BACKGROUND Low	68:81	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.	68:271	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	4	30	theme	Previous	455:462	arg1	studies					464:470	Previous studies	455:470	Previous studies	455:470	Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation.					
29981899	0	31	theme	Plasma	0:5	arg1	composition					17:27	Plasma N-glycome composition	0:27	Plasma N-glycome composition	0:27	Plasma N-glycome composition associates with chronic low back pain.					
29981899	4	32	theme	plasma	535:540	arg1	N-glycosylation					542:556	total plasma N-glycosylation	529:556	total plasma N-glycosylation	529:556	Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation.					
29981899	9	33	contain	containing	1567:1576	arg1	high-mannose					1542:1553	high-mannose	1542:1553	high-mannose	1542:1553	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	9	33	contain	containing	1567:1576	arg2	N-acetylglucosamine					1588:1606	bisecting N-acetylglucosamine	1578:1606	bisecting N-acetylglucosamine	1578:1606	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	8	34	theme	healthy	1420:1426	arg1	controls					1428:1435	healthy controls	1420:1435	healthy controls	1420:1435	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	3	35	theme	underlying	422:431	arg1	process					446:452	an underlying inflammatory process	419:452	an underlying inflammatory process	419:452	LBP may be characterized by an underlying inflammatory process.					
29981899	6	36	theme	clinical	847:854	arg1	centers					856:862	clinical centers	847:862	clinical centers in Italy, Belgium and Croatia	847:892	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	2	37	theme	psychological	368:380	arg1	factors					382:388	psychological factors	368:388	psychological factors	368:388	Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors.					
29981899	7	38	theme	acute	1161:1165	arg1	inflammation					1167:1178	acute inflammation	1161:1178	acute inflammation that underwent abdominal surgery	1161:1211	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	8	39	gly	glycoproteins	1394:1406	arg1	glycoproteins					1394:1406	CLBP patients' plasma glycoproteins	1372:1406	CLBP patients' plasma glycoproteins	1372:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	4	40	theme	inflammatory	503:514	arg1	response					516:523	inflammatory response	503:523	inflammatory response	503:523	Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation.					
29981899	0	41	theme	low	53:55	arg1	pain					62:65	chronic low back pain	45:65	chronic low back pain	45:65	Plasma N-glycome composition associates with chronic low back pain.					
29981899	6	42	theme	controls	820:827	arg1	samples					774:780	METHODS Plasma samples	759:780	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls	759:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	11	43	theme	potential	1931:1939	arg1	pathology					1949:1957	potential disease pathology	1931:1957	potential disease pathology	1931:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	44	dep	SIGNIFICANCE	1784:1795	arg1	knowledge					1804:1812	our knowledge	1800:1812	our knowledge	1800:1812	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	10	45	with	consistent	1698:1707	arg1	changes					1730:1736	N-glycosylation changes	1714:1736	N-glycosylation changes usually seen in chronic inflammation	1714:1773	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	10	46	theme	Observed	1639:1646	arg1	changes					1648:1654	CONCLUSIONS Observed changes	1627:1654	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level	1627:1692	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	9	47	gly	disialylated	1471:1482	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	1	48	theme	treatment	213:221	arg1	selection					223:231	treatment selection	213:231	treatment selection	213:231	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	5	49	theme	chronic	654:660	arg1	CLBP					677:680	CLBP	677:680	CLBP	677:680	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	5	49	theme	chronic	654:660	arg1	pain					671:674	chronic low back pain	654:674	chronic low back pain (CLBP)	654:681	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	9	50	theme	relative	1451:1458	arg1	amounts					1460:1466	relative amounts	1451:1466	relative amounts of disialylated and trisialylated glycan structures	1451:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	9	50	theme	relative	1451:1458	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	6	51	theme	CLBP	790:793	arg1	patients					795:802	1128 CLBP patients	785:802	1128 CLBP patients	785:802	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	8	52	from	glycoproteins	1394:1406	arg1	increase					1264:1271	a statistically significant increase	1236:1271	a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins	1236:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	7	53	with	patients	1147:1154	arg1	inflammation					1167:1178	acute inflammation	1161:1178	acute inflammation that underwent abdominal surgery	1161:1211	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	11	54	theme	GENERAL	1776:1782	arg1	SIGNIFICANCE					1784:1795	GENERAL SIGNIFICANCE	1776:1795	GENERAL SIGNIFICANCE	1776:1795	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	5	55	theme	N-glycosylation	615:629	arg1	pattern					631:637	total plasma N-glycosylation pattern	602:637	total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease	602:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	1	56	theme	heterogeneous	143:155	arg1	mechanisms					168:177	heterogeneous underlying mechanisms	143:177	heterogeneous underlying mechanisms	143:177	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	1	57	theme	syndromes	128:136	arg1	syndromes					128:136	syndromes	128:136	syndromes with heterogeneous underlying mechanisms and molecular pathologies	128:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	1	57	theme	syndromes	128:136	arg1	group					119:123	a group	117:123	a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies	117:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	7	58	theme	patients	1147:1154	arg1	cohort					1133:1138	a cohort	1131:1138	a cohort of 126 patients with acute inflammation that underwent abdominal surgery	1131:1211	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	8	59	theme	significant	1252:1262	arg1	increase					1264:1271	a statistically significant increase	1236:1271	a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins	1236:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	6	60	theme	Plasma	767:772	arg1	samples					774:780	METHODS Plasma samples	759:780	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls	759:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	9	61	theme	glycan	1502:1507	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	6	62	theme	ultra-performance	960:976	arg1	HILIC-UPLC					1001:1010	HILIC-UPLC	1001:1010	HILIC-UPLC	1001:1010	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	62	theme	ultra-performance	960:976	arg1	chromatography					985:998	hydrophilic interaction ultra-performance liquid chromatography	936:998	hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC)	936:1011	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	10	63	theme	plasma	1671:1676	arg1	level					1688:1692	the plasma N-glycome level	1667:1692	the plasma N-glycome level	1667:1692	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	6	64	used	used	898:901	arg2	samples					774:780	METHODS Plasma samples	759:780	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls	759:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	65	theme	hydrophilic	936:946	arg1	HILIC-UPLC					1001:1010	HILIC-UPLC	1001:1010	HILIC-UPLC	1001:1010	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	6	65	theme	hydrophilic	936:946	arg1	chromatography					985:998	hydrophilic interaction ultra-performance liquid chromatography	936:998	hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC)	936:1011	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	9	66	gly	trisialylated	1488:1500	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	8	67	theme	relative	1280:1287	arg1	amount					1289:1294	the relative amount	1276:1294	the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins	1276:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	67	theme	relative	1280:1287	arg1	structures					1358:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	10	68	theme	N-glycosylation	1714:1728	arg1	changes					1730:1736	N-glycosylation changes	1714:1736	N-glycosylation changes usually seen in chronic inflammation	1714:1773	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	9	69	theme	structures	1509:1518	arg1	amounts					1460:1466	relative amounts	1451:1466	relative amounts of disialylated and trisialylated glycan structures	1451:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	9	69	theme	structures	1509:1518	arg1	structures					1509:1518	disialylated and trisialylated glycan structures	1471:1518	disialylated and trisialylated glycan structures	1471:1518	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	1	70	theme	molecular	183:191	arg1	pathologies					193:203	molecular pathologies	183:203	molecular pathologies	183:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	7	71	theme	Observed	1073:1080	arg1	profiles					1098:1105	Observed N-glycosylation profiles	1073:1105	Observed N-glycosylation profiles	1073:1105	Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery.					
29981899	6	72	theme	healthy	812:818	arg1	controls					820:827	760 healthy controls	808:827	760 healthy controls	808:827	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	11	73	theme	glycomics	1906:1914	arg1	perspective					1916:1926	a new glycomics perspective	1900:1926	a new glycomics perspective on potential disease pathology	1900:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	74	from	study	1846:1850	arg1	patients					1860:1867	CLBP patients	1855:1867	CLBP patients	1855:1867	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	74	from	study	1846:1850	arg1	N-glycome					1880:1888	plasma N-glycome	1873:1888	plasma N-glycome	1873:1888	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	9	75	theme	bisecting	1578:1586	arg1	N-acetylglucosamine					1588:1606	bisecting N-acetylglucosamine	1578:1606	bisecting N-acetylglucosamine	1578:1606	Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP.					
29981899	11	76	theme	large	1831:1835	arg1	this					1815:1818	this	1815:1818	this	1815:1818	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	11	76	theme	large	1831:1835	arg1	study					1846:1850	a first large clinical study	1823:1850	a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology	1823:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	1	77	theme	group	119:123	arg1	pain					88:91	back pain	83:91	back pain (LBP)	83:97	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	1	77	theme	group	119:123	arg1	symptom					106:112	the symptom	102:112	the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies	102:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	8	78	theme	structures	1358:1367	arg1	amount					1289:1294	the relative amount	1276:1294	the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins	1276:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	78	theme	structures	1358:1367	arg1	structures					1358:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	high-branched (tri-antennary and tetra-antennary) N-glycan structures	1299:1367	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	79	dep	high-branched	1299:1311	arg1	tetra-antennary					1332:1346	tetra-antennary	1332:1346	tetra-antennary	1332:1346	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	79	dep	high-branched	1299:1311	arg1	tri-antennary					1314:1326	tri-antennary	1314:1326	tri-antennary	1314:1326	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	8	80	theme	CLBP	1372:1375	arg1	patients					1377:1384	CLBP patients'	1372:1385	CLBP patients' plasma glycoproteins	1372:1406	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	2	81	theme	LBP	309:311	arg1	prognosis					296:304	prognosis	296:304	prognosis	296:304	Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors.					
29981899	2	81	theme	LBP	309:311	arg1	symptoms					283:290	symptoms	283:290	symptoms	283:290	Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors.					
29981899	5	82	theme	plasma	608:613	arg1	pattern					631:637	total plasma N-glycosylation pattern	602:637	total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease	602:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	11	83	theme	CLBP	1855:1858	arg1	patients					1860:1867	CLBP patients	1855:1867	CLBP patients	1855:1867	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	5	84	from	changes	591:597	arg1	pattern					631:637	total plasma N-glycosylation pattern	602:637	total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease	602:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	5	85	theme	potential	581:589	arg1	changes					591:597	potential changes	581:597	potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease	581:756	We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.					
29981899	3	86	theme	inflammatory	433:444	arg1	process					446:452	an underlying inflammatory process	419:452	an underlying inflammatory process	419:452	LBP may be characterized by an underlying inflammatory process.					
29981899	0	87	theme	chronic	45:51	arg1	pain					62:65	chronic low back pain	45:65	chronic low back pain	45:65	Plasma N-glycome composition associates with chronic low back pain.					
29981899	6	88	theme	fluorescent	1038:1048	arg1	labeling					1050:1057	fluorescent labeling	1038:1057	fluorescent labeling	1038:1057	METHODS Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up.					
29981899	10	89	theme	chronic	1754:1760	arg1	inflammation					1762:1773	chronic inflammation	1754:1773	chronic inflammation	1754:1773	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	4	90	theme	total	529:533	arg1	N-glycosylation					542:556	total plasma N-glycosylation	529:556	total plasma N-glycosylation	529:556	Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation.					
29981899	8	91	dep	RESULTS	1214:1220	arg1	found					1230:1234	found	1230:1234	have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls	1225:1435	RESULTS We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls.					
29981899	1	92	with	syndromes	128:136	arg1	mechanisms					168:177	heterogeneous underlying mechanisms	143:177	heterogeneous underlying mechanisms	143:177	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	1	92	with	syndromes	128:136	arg1	pathologies					193:203	molecular pathologies	183:203	molecular pathologies	183:203	BACKGROUND Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging.					
29981899	10	93	from	changes	1648:1654	arg1	level					1688:1692	the plasma N-glycome level	1667:1692	the plasma N-glycome level	1667:1692	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	10	93	from	changes	1648:1654	arg1	CLBP					1659:1662	CLBP	1659:1662	CLBP	1659:1662	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	11	94	theme	disease	1941:1947	arg1	pathology					1949:1957	potential disease pathology	1931:1957	potential disease pathology	1931:1957	GENERAL SIGNIFICANCE To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.					
29981899	10	95	theme	CONCLUSIONS	1627:1637	arg1	changes					1648:1654	CONCLUSIONS Observed changes	1627:1654	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level	1627:1692	CONCLUSIONS Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation.					
29981899	0	96	theme	N-glycome	7:15	arg1	composition					17:27	Plasma N-glycome composition	0:27	Plasma N-glycome composition	0:27	Plasma N-glycome composition associates with chronic low back pain.					
29324282	5	0	gly	deglycosylated	863:876	arg1	IgG1					851:854	IgG1	851:854	IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated)	851:918	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	1	1	theme	biopharmaceuticals	143:160	arg1	antibodies					92:101	Monoclonal antibodies	81:101	Monoclonal antibodies (mAbs)	81:108	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	1	1	theme	biopharmaceuticals	143:160	arg1	class					134:138	the fastest growing class	114:138	the fastest growing class of biopharmaceuticals	114:160	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	8	2	theme	measurable	1652:1661	arg1	binding					1663:1669	no measurable binding	1649:1669	no measurable binding	1649:1669	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	8	3	theme	antibody	1779:1786	arg1	form					1767:1770	this form	1762:1770	this form of the antibody	1762:1786	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	7	4	theme	significant	1318:1328	arg1	changes					1330:1336	No significant changes	1315:1336	No significant changes	1315:1336	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	5	5	theme	mAb	977:979	arg1	molecule					981:988	the unmodified mAb molecule	962:988	the unmodified mAb molecule	962:988	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	0	6	from	Influence	0:8	arg1	properties					69:78	IgG1 biochemical and biophysical properties	36:78	IgG1 biochemical and biophysical properties	36:78	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	3	7	dep	defines	386:392	arg1	irrelevant					521:530	irrelevant	521:530	irrelevant	521:530	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	3	7	dep	defines	386:392	arg1	critical					479:486	critical	479:486	critical	479:486	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	7	8	theme	hypergalactosylated	1360:1378	arg1	FcγRIIIA					1459:1466	FcγRIIIA	1459:1466	FcγRIIIA	1459:1466	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	8	theme	hypergalactosylated	1360:1378	arg1	FcγRIIA					1447:1453	FcγRIIA	1447:1453	FcγRIIA	1447:1453	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	8	theme	hypergalactosylated	1360:1378	arg1	forms					1400:1404	the hypergalactosylated and hypersialylated forms	1356:1404	the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn	1356:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	2	9	theme	cytotoxic	288:296	arg1	pathways					298:305	cytotoxic pathways	288:305	cytotoxic pathways	288:305	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	7	10	theme	IgG1	1409:1412	arg1	FcγRIIIA					1459:1466	FcγRIIIA	1459:1466	FcγRIIIA	1459:1466	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	10	theme	IgG1	1409:1412	arg1	FcγRIIA					1447:1453	FcγRIIA	1447:1453	FcγRIIA	1447:1453	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	10	theme	IgG1	1409:1412	arg1	forms					1400:1404	the hypergalactosylated and hypersialylated forms	1356:1404	the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn	1356:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	3	11	theme	specific	360:367	arg1	goal					381:384	The specific therapeutic goal	356:384	The specific therapeutic goal	356:384	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	6	12	theme	reduced	1255:1261	arg1	stability					1271:1279	reduced thermal stability	1255:1279	reduced thermal stability	1255:1279	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	13	theme	higher	1090:1095	arg1	structure					1103:1111	the higher order structure	1086:1111	the higher order structure	1086:1111	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	5	14	theme	glycan-modified	826:840	arg1	forms					842:846	Three different glycan-modified forms	810:846	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated)	810:918	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	4	15	theme	Fc	689:690	arg1	families					663:670	two key receptor families	646:670	two key receptor families	646:670	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	15	theme	Fc	689:690	arg1	receptors					692:700	γ-type Fc receptors	682:700	γ-type Fc receptors	682:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	16	from	influence	718:726	arg1	conformation					757:768	conformation	757:768	conformation	757:768	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	16	from	influence	718:726	arg1	stability					774:782	stability	774:782	stability	774:782	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	17	theme	composition	738:748	arg1	influence					718:726	the influence	714:726	the influence of glycan composition on the conformation and stability of the antibody molecule	714:807	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	9	18	theme	Fc	1882:1883	arg1	recognition					1894:1904	Fc receptor recognition	1882:1904	Fc receptor recognition	1882:1904	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	3	19	theme	multiple	438:445	arg1	partners					461:468	its multiple physiological partners	434:468	its multiple physiological partners	434:468	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	7	20	theme	immune	1549:1554	arg1	response					1556:1563	an immune response	1546:1563	an immune response	1546:1563	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	4	21	theme	molecule	800:807	arg1	conformation					757:768	conformation	757:768	conformation	757:768	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	21	theme	molecule	800:807	arg1	stability					774:782	stability	774:782	stability	774:782	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	1	22	theme	Monoclonal	81:90	arg1	mAbs					104:107	mAbs	104:107	mAbs	104:107	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	1	22	theme	Monoclonal	81:90	arg1	class					134:138	the fastest growing class	114:138	the fastest growing class of biopharmaceuticals	114:160	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	1	22	theme	Monoclonal	81:90	arg1	antibodies					92:101	Monoclonal antibodies	81:101	Monoclonal antibodies (mAbs)	81:108	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	9	23	theme	receptor	1885:1892	arg1	recognition					1894:1904	Fc receptor recognition	1882:1904	Fc receptor recognition	1882:1904	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	3	24	theme	physiological	447:459	arg1	partners					461:468	its multiple physiological partners	434:468	its multiple physiological partners	434:468	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	7	25	theme	glycan	1621:1626	arg1	chains					1628:1633	the glycan chains	1617:1633	the glycan chains	1617:1633	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	26	theme	chains	1628:1633	arg1	modifications					1600:1612	these modifications	1594:1612	these modifications of the glycan chains	1594:1633	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	2	27	theme	drug	317:320	arg1	delivery					322:329	targeted drug delivery	308:329	targeted drug delivery	308:329	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	8	28	theme	deglycosylated	1692:1705	arg1	form					1707:1710	the deglycosylated form	1688:1710	the deglycosylated form of IgG1	1688:1718	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	4	29	theme	receptor	654:661	arg1	FcRn					673:676	FcRn	673:676	FcRn	673:676	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	29	theme	receptor	654:661	arg1	families					663:670	two key receptor families	646:670	two key receptor families	646:670	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	29	theme	receptor	654:661	arg1	receptors					692:700	γ-type Fc receptors	682:700	γ-type Fc receptors	682:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	7	30	with	IgG1	1409:1412	arg1	regards					1419:1425	regards	1419:1425	regards to binding to FcRn	1419:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	3	31	with	interactions	400:411	arg1	partners					461:468	its multiple physiological partners	434:468	its multiple physiological partners	434:468	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	8	32	located	observed	1675:1682	arg2	binding					1663:1669	no measurable binding	1649:1669	no measurable binding	1649:1669	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	8	32	located	observed	1675:1682	arg1	contrast					1639:1646	contrast	1639:1646	contrast	1639:1646	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	9	33	theme	attenuation	1867:1877	arg1	patterns					1855:1862	These highly specific patterns	1833:1862	These highly specific patterns of attenuation of Fc receptor recognition	1833:1904	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	2	34	theme	targeted	308:315	arg1	delivery					322:329	targeted drug delivery	308:329	targeted drug delivery	308:329	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	8	35	theme	IgG1	1715:1718	arg1	form					1707:1710	the deglycosylated form	1688:1710	the deglycosylated form of IgG1	1688:1718	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	4	36	theme	key	650:652	arg1	FcRn					673:676	FcRn	673:676	FcRn	673:676	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	36	theme	key	650:652	arg1	families					663:670	two key receptor families	646:670	two key receptor families	646:670	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	36	theme	key	650:652	arg1	receptors					692:700	γ-type Fc receptors	682:700	γ-type Fc receptors	682:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	0	37	theme	modification	20:31	arg1	Influence					0:8	Influence	0:8	Influence of glycan modification on IgG1 biochemical and biophysical properties.	0:79	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	6	38	theme	alterations	1071:1081	arg1	indicative					1057:1066	indicative	1057:1066	indicative	1057:1066	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	39	from	alterations	1071:1081	arg1	structure					1103:1111	the higher order structure	1086:1111	the higher order structure	1086:1111	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	39	from	alterations	1071:1081	arg1	propensity					1138:1147	increased aggregation propensity	1116:1147	increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb	1116:1210	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	0	40	theme	glycan	13:18	arg1	modification					20:31	glycan modification	13:31	glycan modification	13:31	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	5	41	theme	unmodified	966:975	arg1	molecule					981:988	the unmodified mAb molecule	962:988	the unmodified mAb molecule	962:988	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	2	42	theme	pathways	298:305	arg1	imaging					347:353	diagnostic imaging	336:353	diagnostic imaging	336:353	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	42	theme	pathways	298:305	arg1	delivery					322:329	targeted drug delivery	308:329	targeted drug delivery	308:329	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	42	theme	pathways	298:305	arg1	activation					274:283	activation	274:283	activation of cytotoxic pathways	274:305	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	42	theme	pathways	298:305	arg1	neutralization					239:252	neutralization	239:252	neutralization of soluble targets	239:271	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	9	43	theme	therapeutic	1941:1951	arg1	purposes					1953:1960	therapeutic purposes	1941:1960	therapeutic purposes	1941:1960	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	0	44	theme	IgG1	36:39	arg1	properties					69:78	IgG1 biochemical and biophysical properties	36:78	IgG1 biochemical and biophysical properties	36:78	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	6	45	theme	aggregation	1126:1136	arg1	propensity					1138:1147	increased aggregation propensity	1116:1147	increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb	1116:1210	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	8	46	gly	deglycosylated	1692:1705	arg1	IgG1					1715:1718	IgG1	1715:1718	IgG1	1715:1718	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	8	46	gly	deglycosylated	1692:1705	arg1	form					1707:1710	the deglycosylated form	1688:1710	the deglycosylated form of IgG1	1688:1718	In contrast, no measurable binding was observed for the deglycosylated form of IgG1 with either FcγRIIA or FcγRIIIA, although this form of the antibody retained the ability to associate with FcRn.					
29324282	2	47	theme	therapeutic	176:186	arg1	tasks					188:192	The specific therapeutic tasks	163:192	The specific therapeutic tasks	163:192	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	7	48	theme	hypersialylated	1384:1398	arg1	FcγRIIIA					1459:1466	FcγRIIIA	1459:1466	FcγRIIIA	1459:1466	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	48	theme	hypersialylated	1384:1398	arg1	FcγRIIA					1447:1453	FcγRIIA	1447:1453	FcγRIIA	1447:1453	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	48	theme	hypersialylated	1384:1398	arg1	forms					1400:1404	the hypergalactosylated and hypersialylated forms	1356:1404	the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn	1356:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	5	49	theme	IgG1	851:854	arg1	forms					842:846	Three different glycan-modified forms	810:846	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated)	810:918	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	6	50	contain	have	1250:1253	arg2	stability					1271:1279	reduced thermal stability	1255:1279	reduced thermal stability	1255:1279	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	50	contain	have	1250:1253	arg1	domain					1230:1235	the CH2 domain	1222:1235	the CH2 domain	1222:1235	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	3	51	theme	antibody	420:427	arg1	interactions					400:411	interactions	400:411	interactions of the antibody with its multiple physiological partners	400:468	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	6	52	theme	Biophysical	991:1001	arg1	measurements					1003:1014	Biophysical measurements	991:1014	Biophysical measurements	991:1014	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	2	53	theme	specific	167:174	arg1	tasks					188:192	The specific therapeutic tasks	163:192	The specific therapeutic tasks	163:192	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	3	54	theme	therapeutic	369:379	arg1	goal					381:384	The specific therapeutic goal	356:384	The specific therapeutic goal	356:384	The specific therapeutic goal defines which interactions of the antibody with its multiple physiological partners are most critical for function, and which ones are irrelevant or indeed detrimental.					
29324282	9	55	theme	recognition	1894:1904	arg1	attenuation					1867:1877	attenuation	1867:1877	attenuation of Fc receptor recognition	1867:1904	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	4	56	theme	IgG1	624:627	arg1	interaction					629:639	IgG1 interaction	624:639	IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors	624:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	57	theme	antibody	791:798	arg1	molecule					800:807	the antibody molecule	787:807	the antibody molecule	787:807	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	2	58	theme	diagnostic	336:345	arg1	imaging					347:353	diagnostic imaging	336:353	diagnostic imaging	336:353	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	0	59	theme	biochemical	41:51	arg1	properties					69:78	IgG1 biochemical and biophysical properties	36:78	IgG1 biochemical and biophysical properties	36:78	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	6	60	theme	CH2	1226:1228	arg1	domain					1230:1235	the CH2 domain	1222:1235	the CH2 domain	1222:1235	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	61	theme	deglycosylated	1294:1307	arg1	form					1309:1312	the fully deglycosylated form	1284:1312	the fully deglycosylated form	1284:1312	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	4	62	with	interaction	629:639	arg1	FcRn					673:676	FcRn	673:676	FcRn	673:676	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	62	with	interaction	629:639	arg1	families					663:670	two key receptor families	646:670	two key receptor families	646:670	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	62	with	interaction	629:639	arg1	receptors					692:700	γ-type Fc receptors	682:700	γ-type Fc receptors	682:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	63	theme	γ-type	682:687	arg1	families					663:670	two key receptor families	646:670	two key receptor families	646:670	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	63	theme	γ-type	682:687	arg1	receptors					692:700	γ-type Fc receptors	682:700	γ-type Fc receptors	682:700	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	2	64	theme	different	205:213	arg1	mAbs					215:218	different mAbs	205:218	different mAbs	205:218	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	65	theme	targets	265:271	arg1	imaging					347:353	diagnostic imaging	336:353	diagnostic imaging	336:353	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	65	theme	targets	265:271	arg1	delivery					322:329	targeted drug delivery	308:329	targeted drug delivery	308:329	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	65	theme	targets	265:271	arg1	activation					274:283	activation	274:283	activation of cytotoxic pathways	274:305	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	2	65	theme	targets	265:271	arg1	neutralization					239:252	neutralization	239:252	neutralization of soluble targets	239:271	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	5	66	dep	IgG1	851:854	arg1	deglycosylated					863:876	deglycosylated	863:876	deglycosylated	863:876	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	5	66	dep	IgG1	851:854	arg1	hypersialylated					903:917	hypersialylated	903:917	hypersialylated	903:917	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	5	66	dep	IgG1	851:854	arg1	hypergalactosylated					879:897	hypergalactosylated	879:897	hypergalactosylated	879:897	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	1	67	theme	fastest	118:124	arg1	antibodies					92:101	Monoclonal antibodies	81:101	Monoclonal antibodies (mAbs)	81:108	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	1	67	theme	fastest	118:124	arg1	class					134:138	the fastest growing class	114:138	the fastest growing class of biopharmaceuticals	114:160	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	4	68	theme	glycan	731:736	arg1	composition					738:748	glycan composition	731:748	glycan composition	731:748	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	2	69	theme	soluble	257:263	arg1	targets					265:271	soluble targets	257:271	soluble targets	257:271	The specific therapeutic tasks vary among different mAbs, which may include neutralization of soluble targets, activation of cytotoxic pathways, targeted drug delivery, and diagnostic imaging.					
29324282	1	70	theme	growing	126:132	arg1	antibodies					92:101	Monoclonal antibodies	81:101	Monoclonal antibodies (mAbs)	81:108	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	1	70	theme	growing	126:132	arg1	class					134:138	the fastest growing class	114:138	the fastest growing class of biopharmaceuticals	114:160	Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals.					
29324282	6	71	gly	deglycosylated	1294:1307	arg1	form					1309:1312	the fully deglycosylated form	1284:1312	the fully deglycosylated form	1284:1312	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	72	theme	increased	1116:1124	arg1	propensity					1138:1147	increased aggregation propensity	1116:1147	increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb	1116:1210	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	7	73	from	half-life	1493:1501	arg1	circulation					1506:1516	circulation	1506:1516	circulation	1506:1516	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	5	74	theme	different	816:824	arg1	forms					842:846	Three different glycan-modified forms	810:846	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated)	810:918	Three different glycan-modified forms of IgG1 (fully deglycosylated, hypergalactosylated and hypersialylated) were produced and characterized alongside the unmodified mAb molecule.					
29324282	6	75	theme	unmodified	1197:1206	arg1	mAb					1208:1210	the unmodified mAb	1193:1210	the unmodified mAb	1193:1210	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	9	76	theme	specific	1846:1853	arg1	patterns					1855:1862	These highly specific patterns	1833:1862	These highly specific patterns of attenuation of Fc receptor recognition	1833:1904	These highly specific patterns of attenuation of Fc receptor recognition can be exploited in the future for therapeutic purposes.					
29324282	7	77	gly	hypersialylated	1384:1398	arg1	FcγRIIA					1447:1453	FcγRIIA	1447:1453	FcγRIIA	1447:1453	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	77	gly	hypersialylated	1384:1398	arg1	FcγRIIIA					1459:1466	FcγRIIIA	1459:1466	FcγRIIIA	1459:1466	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	77	gly	hypersialylated	1384:1398	arg1	IgG1					1409:1412	IgG1	1409:1412	IgG1 with regards to binding to FcRn	1409:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	7	77	gly	hypersialylated	1384:1398	arg1	forms					1400:1404	the hypergalactosylated and hypersialylated forms	1356:1404	the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn	1356:1444	No significant changes were observed for the hypergalactosylated and hypersialylated forms of IgG1 with regards to binding to FcRn, FcγRIIA and FcγRIIIA, suggesting that neither half-life in circulation nor their ability to induce an immune response are likely to be affected by these modifications of the glycan chains.					
29324282	4	78	dep	conformation	757:768	arg1	the					753:755	the	753:755	the	753:755	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	4	79	theme	chains	607:612	arg1	ability					585:591	the ability	581:591	the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule	581:807	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
29324282	0	80	theme	biophysical	57:67	arg1	properties					69:78	IgG1 biochemical and biophysical properties	36:78	IgG1 biochemical and biophysical properties	36:78	Influence of glycan modification on IgG1 biochemical and biophysical properties.					
29324282	6	81	theme	order	1097:1101	arg1	structure					1103:1111	the higher order structure	1086:1111	the higher order structure	1086:1111	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	6	82	theme	thermal	1263:1269	arg1	stability					1271:1279	reduced thermal stability	1255:1279	reduced thermal stability	1255:1279	Biophysical measurements did not reveal any changes that would be indicative of alterations in the higher order structure or increased aggregation propensity for any of the three glycoforms compared to the unmodified mAb, although the CH2 domain was shown to have reduced thermal stability in the fully deglycosylated form.					
29324282	4	83	theme	glycan	600:605	arg1	chains					607:612	the glycan chains	596:612	the glycan chains	596:612	In this work, we explored the ability of the glycan chains to affect IgG1 interaction with two key receptor families, FcRn and γ-type Fc receptors, as well as the influence of glycan composition on the conformation and stability of the antibody molecule.					
30574787	2	0	from	tables	425:430	arg1	publications					435:446	publications	435:446	publications	435:446	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	1	1	theme	glycan	150:155	arg1	structures					157:166	the glycan structures	146:166	the glycan structures that they present to their recognition partners in health and disease	146:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	3	2	theme	interactomics	693:705	arg1	information					707:717	interactomics information	693:717	interactomics information	693:717	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	3	3	theme	genomics	679:686	arg1	sources					648:654	other sources	642:654	other sources of related proteomics, genomics, and interactomics information	642:717	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	1	4	theme	structures	157:166	arg1	Knowledge					70:78	Knowledge	70:78	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease	70:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	1	5	gly	glycoproteins	83:95	arg1	glycoproteins					83:95	glycoproteins	83:95	glycoproteins	83:95	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	5	6	theme	user-friendly	944:956	arg1	way					958:960	a user-friendly way	942:960	a user-friendly way to facilitate the interpretation of collected glycoscience data	942:1024	GlyConnect has been developed to gather, monitor, integrate, and visualize data in a user-friendly way to facilitate the interpretation of collected glycoscience data.					
30574787	2	7	from	servers	461:467	arg1	laboratories					485:496	investigator laboratories	472:496	investigator laboratories	472:496	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	2	8	from	analyses	328:335	arg1	data					312:315	The data	308:315	The data from these analyses	308:335	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	3	9	theme	Bioinformatics	499:512	arg1	tools					514:518	Bioinformatics tools	499:518	Bioinformatics tools	499:518	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	2	10	theme	various	390:396	arg1	sources					398:404	various sources	390:404	various sources	390:404	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	3	11	theme	information	707:717	arg1	sources					648:654	other sources	642:654	other sources of related proteomics, genomics, and interactomics information	642:717	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	4	12	theme	portal	830:835	arg1	platform					797:804	the central platform	785:804	the central platform of the Glycomics@ExPASy portal for glycoinformatics	785:856	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	6	13	theme	multiple	1079:1086	arg1	types					1093:1097	multiple data types	1079:1097	multiple data types	1079:1097	GlyConnect is designed to accommodate and integrate multiple data types as they are increasingly produced.					
30574787	4	14	dep	https	751:755	arg1	//glyconnect.expasy.org/					757:780	//glyconnect.expasy.org/	757:780	https://glyconnect.expasy.org/	751:780	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	1	15	theme	glycoproteins	83:95	arg1	Knowledge					70:78	Knowledge	70:78	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease	70:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	5	16	theme	glycoscience	1008:1019	arg1	data					1021:1024	collected glycoscience data	998:1024	collected glycoscience data	998:1024	GlyConnect has been developed to gather, monitor, integrate, and visualize data in a user-friendly way to facilitate the interpretation of collected glycoscience data.					
30574787	2	17	theme	supplemental	412:423	arg1	tables					425:430	supplemental tables	412:430	supplemental tables in publications	412:446	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	3	18	theme	other	642:646	arg1	sources					648:654	other sources	642:654	other sources of related proteomics, genomics, and interactomics information	642:717	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	1	19	theme	recognition	195:205	arg1	partners					207:214	their recognition partners	189:214	their recognition partners in health and disease	189:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	2	20	theme	investigator	472:483	arg1	laboratories					485:496	investigator laboratories	472:496	investigator laboratories	472:496	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	4	21	theme	Glycomics	813:821	arg1	portal					830:835	the Glycomics@ExPASy portal	809:835	the Glycomics@ExPASy portal	809:835	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	6	22	theme	data	1088:1091	arg1	types					1093:1097	multiple data types	1079:1097	multiple data types	1079:1097	GlyConnect is designed to accommodate and integrate multiple data types as they are increasingly produced.					
30574787	1	23	theme	experimental	283:294	arg1	approaches					296:305	experimental approaches	283:305	experimental approaches	283:305	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	5	24	theme	data	1021:1024	arg1	interpretation					980:993	the interpretation	976:993	the interpretation of collected glycoscience data	976:1024	GlyConnect has been developed to gather, monitor, integrate, and visualize data in a user-friendly way to facilitate the interpretation of collected glycoscience data.					
30574787	1	25	from	partners	207:214	arg1	disease					230:236	disease	230:236	disease	230:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	1	25	from	partners	207:214	arg1	health					219:224	health	219:224	health	219:224	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	1	26	theme	approaches	296:305	arg1	range					274:278	a range	272:278	a range of experimental approaches	272:305	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	4	27	theme	ExPASy	823:828	arg1	portal					830:835	the Glycomics@ExPASy portal	809:835	the Glycomics@ExPASy portal	809:835	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	1	28	theme	site-specific	104:116	arg1	patterns					132:139	their site-specific glycosylation patterns	98:139	their site-specific glycosylation patterns	98:139	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	2	29	theme	localized	451:459	arg1	servers					461:467	localized servers	451:467	localized servers in investigator laboratories	451:496	The data from these analyses are increasingly being standardized and presented in various sources, from supplemental tables in publications to localized servers in investigator laboratories.					
30574787	3	30	theme	proteomics	667:676	arg1	sources					648:654	other sources	642:654	other sources of related proteomics, genomics, and interactomics information	642:717	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	4	31	theme	@	822:822	arg1	portal					830:835	the Glycomics@ExPASy portal	809:835	the Glycomics@ExPASy portal	809:835	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	1	32	theme	glycosylation	118:130	arg1	patterns					132:139	their site-specific glycosylation patterns	98:139	their site-specific glycosylation patterns	98:139	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	5	33	theme	collected	998:1006	arg1	data					1021:1024	collected glycoscience data	998:1024	collected glycoscience data	998:1024	GlyConnect has been developed to gather, monitor, integrate, and visualize data in a user-friendly way to facilitate the interpretation of collected glycoscience data.					
30574787	4	34	theme	central	789:795	arg1	platform					797:804	the central platform	785:804	the central platform of the Glycomics@ExPASy portal for glycoinformatics	785:856	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	1	35	theme	patterns	132:139	arg1	Knowledge					70:78	Knowledge	70:78	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease	70:236	Knowledge of glycoproteins, their site-specific glycosylation patterns, and the glycan structures that they present to their recognition partners in health and disease is gradually being built on using a range of experimental approaches.					
30574787	5	36	dep	way	958:960	arg1	facilitate					965:974	facilitate	965:974	to facilitate the interpretation of collected glycoscience data	962:1024	GlyConnect has been developed to gather, monitor, integrate, and visualize data in a user-friendly way to facilitate the interpretation of collected glycoscience data.					
30574787	4	37	dep	GlyConnect	738:747	arg1	https					751:755	https	751:755	https://glyconnect.expasy.org/	751:780	We here introduce GlyConnect ( https://glyconnect.expasy.org/ ), the central platform of the Glycomics@ExPASy portal for glycoinformatics.					
30574787	3	38	theme	related	659:665	arg1	proteomics					667:676	related proteomics	659:676	related proteomics	659:676	Bioinformatics tools are now needed to collect these data and enable the user to search, display, and connect glycomics and glycoproteomics to other sources of related proteomics, genomics, and interactomics information.					
30574787	0	39	dep	GlyConnect	0:9	arg1	Glycoproteomics					12:26	Glycoproteomics	12:26	GlyConnect: Glycoproteomics	0:26	GlyConnect: Glycoproteomics Goes Visual, Interactive, and Analytical.					
29301962	1	0	from	reticulum	196:204	arg1	complex					169:175	complex	169:175	complex	169:175	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	4	1	theme	membrane-proximal	743:759	arg1	pocket					761:766	a membrane-proximal pocket	741:766	a membrane-proximal pocket for the oligosaccharide	741:790	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	2	2	theme	conserved	440:448	arg1	arrangement					458:468	a conserved subunit arrangement	438:468	a conserved subunit arrangement	438:468	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
29301962	5	3	from	domain	847:852	arg1	complexes					873:881	stand-alone OST complexes	857:881	stand-alone OST complexes	857:881	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	1	4	theme	glycosylation	294:306	arg1	sites					308:312	glycosylation sites	294:312	glycosylation sites of secretory proteins	294:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	4	theme	glycosylation	294:306	arg1	proteins					327:334	secretory proteins	317:334	secretory proteins	317:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	5	5	theme	polypeptides	1002:1013	arg1	number					992:997	a large number	984:997	a large number of polypeptides	984:1013	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	5	6	theme	acceptor	797:804	arg1	adjacent					901:908	adjacent	901:908	adjacent	901:908	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	5	6	theme	acceptor	797:804	arg1	site					818:821	The acceptor polypeptide site	793:821	The acceptor polypeptide site	793:821	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	5	7	theme	large	986:990	arg1	number					992:997	a large number	984:997	a large number of polypeptides	984:1013	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	1	8	from	complex	169:175	arg1	reticulum					196:204	the endoplasmic reticulum	180:204	the endoplasmic reticulum	180:204	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	4	9	theme	ordered	719:725	arg1	N-glycan					727:734	an ordered N-glycan	716:734	an ordered N-glycan	716:734	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	1	10	theme	endoplasmic	184:194	arg1	reticulum					196:204	the endoplasmic reticulum	180:204	the endoplasmic reticulum	180:204	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	5	11	theme	polypeptide	806:816	arg1	adjacent					901:908	adjacent	901:908	adjacent	901:908	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	5	11	theme	polypeptide	806:816	arg1	site					818:821	The acceptor polypeptide site	793:821	The acceptor polypeptide site	793:821	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	1	12	gly	glycosylation	294:306	arg2	sites					308:312	glycosylation sites	294:312	glycosylation sites of secretory proteins	294:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	12	gly	glycosylation	294:306	arg1	proteins					327:334	secretory proteins	317:334	secretory proteins	317:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	12	gly	glycosylation	294:306	arg2	proteins					327:334	secretory proteins	317:334	secretory proteins	317:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	3	13	theme	unhindered	570:579	arg1	access					581:586	unhindered access	570:586	unhindered access to substrates	570:600	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	5	14	theme	oxidoreductase	832:845	arg1	domain					847:852	an oxidoreductase domain	829:852	an oxidoreductase domain in stand-alone OST complexes	829:881	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	2	15	theme	atomic	358:363	arg1	structure					365:373	the atomic structure	354:373	the atomic structure of yeast OST determined by cryo-electron microscopy	354:425	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
29301962	3	16	theme	active	475:480	arg1	subunit					509:515	the catalytic STT3 subunit	490:515	the catalytic STT3 subunit	490:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	3	16	theme	active	475:480	arg1	site					482:485	The active site	471:485	The active site of the catalytic STT3 subunit	471:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	1	17	theme	secretory	317:325	arg1	proteins					327:334	secretory proteins	317:334	secretory proteins	317:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	5	18	theme	eukaryotic	944:953	arg1	OSTs					955:958	eukaryotic OSTs	944:958	eukaryotic OSTs	944:958	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	0	19	theme	yeast	17:21	arg1	complex					49:55	the yeast oligosaccharyltransferase complex	13:55	the yeast oligosaccharyltransferase complex	13:55	Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation.					
29301962	1	20	theme	proteins	327:334	arg1	sites					308:312	glycosylation sites	294:312	glycosylation sites of secretory proteins	294:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	20	theme	proteins	327:334	arg1	proteins					327:334	secretory proteins	317:334	secretory proteins	317:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	3	21	theme	subunit	509:515	arg1	subunit					509:515	the catalytic STT3 subunit	490:515	the catalytic STT3 subunit	490:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	3	21	theme	subunit	509:515	arg1	site					482:485	The active site	471:485	The active site of the catalytic STT3 subunit	471:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	4	22	theme	dolichol-pyrophosphate	607:628	arg1	moiety					630:635	The dolichol-pyrophosphate moiety	603:635	The dolichol-pyrophosphate moiety	603:635	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	0	23	theme	complex	49:55	arg1	Structure					0:8	Structure	0:8	Structure of the yeast oligosaccharyltransferase complex	0:55	Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation.					
29301962	4	24	theme	noncatalytic	690:701	arg1	subunits					703:710	two noncatalytic subunits	686:710	two noncatalytic subunits	686:710	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	3	25	theme	catalytic	494:502	arg1	subunit					509:515	the catalytic STT3 subunit	490:515	the catalytic STT3 subunit	490:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	0	26	theme	oligosaccharyltransferase	23:47	arg1	complex					49:55	the yeast oligosaccharyltransferase complex	13:55	the yeast oligosaccharyltransferase complex	13:55	Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation.					
29301962	4	27	theme	STT3	672:675	arg1	groove					662:667	a lipid-exposed groove	646:667	a lipid-exposed groove of STT3	646:675	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	3	28	theme	STT3	504:507	arg1	subunit					509:515	the catalytic STT3 subunit	490:515	the catalytic STT3 subunit	490:515	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	5	29	theme	stand-alone	857:867	arg1	complexes					873:881	stand-alone OST complexes	857:881	stand-alone OST complexes	857:881	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	2	30	theme	OST	384:386	arg1	structure					365:373	the atomic structure	354:373	the atomic structure of yeast OST determined by cryo-electron microscopy	354:425	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
29301962	5	31	theme	OST	869:871	arg1	complexes					873:881	stand-alone OST complexes	857:881	stand-alone OST complexes	857:881	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	1	32	theme	complex	169:175	arg1	Oligosaccharyltransferase					104:128	Oligosaccharyltransferase	104:128	Oligosaccharyltransferase (OST)	104:134	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	32	theme	complex	169:175	arg1	protein					161:167	an essential membrane protein	139:167	an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins	139:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	2	33	theme	yeast	378:382	arg1	OST					384:386	yeast OST	378:386	yeast OST determined by cryo-electron microscopy	378:425	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
29301962	1	34	theme	essential	142:150	arg1	Oligosaccharyltransferase					104:128	Oligosaccharyltransferase	104:128	Oligosaccharyltransferase (OST)	104:134	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	34	theme	essential	142:150	arg1	protein					161:167	an essential membrane protein	139:167	an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins	139:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	5	35	gly	glycosylate	972:982	arg1	number					992:997	a large number	984:997	a large number of polypeptides	984:1013	The acceptor polypeptide site faces an oxidoreductase domain in stand-alone OST complexes or is immediately adjacent to the translocon, suggesting how eukaryotic OSTs efficiently glycosylate a large number of polypeptides before their folding.					
29301962	1	36	theme	membrane	152:159	arg1	Oligosaccharyltransferase					104:128	Oligosaccharyltransferase	104:128	Oligosaccharyltransferase (OST)	104:134	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	1	36	theme	membrane	152:159	arg1	protein					161:167	an essential membrane protein	139:167	an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins	139:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	4	37	theme	lipid-exposed	648:660	arg1	groove					662:667	a lipid-exposed groove	646:667	a lipid-exposed groove of STT3	646:675	The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, whereas two noncatalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide.					
29301962	0	38	theme	eukaryotic	76:85	arg1	N-glycosylation					87:101	eukaryotic N-glycosylation	76:101	eukaryotic N-glycosylation	76:101	Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation.					
29301962	2	39	theme	subunit	450:456	arg1	arrangement					458:468	a conserved subunit arrangement	438:468	a conserved subunit arrangement	438:468	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
29301962	3	40	theme	complex	552:558	arg1	center					538:543	the center	534:543	the center of the complex	534:558	The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates.					
29301962	1	41	theme	dolichol-pyrophosphate-activated	252:283	arg1	donor					285:289	a dolichol-pyrophosphate-activated donor	250:289	a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins	250:334	Oligosaccharyltransferase (OST) is an essential membrane protein complex in the endoplasmic reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins.					
29301962	2	42	theme	cryo-electron	402:414	arg1	microscopy					416:425	cryo-electron microscopy	402:425	cryo-electron microscopy	402:425	Here we describe the atomic structure of yeast OST determined by cryo-electron microscopy, revealing a conserved subunit arrangement.					
31381874	8	0	theme	mammalian	1476:1484	arg1	system					1502:1507	a mammalian cell expression system	1474:1507	a mammalian cell expression system	1474:1507	This platform methodology can be readily adapted for any human IgM produced in a mammalian cell expression system.					
31381874	7	1	theme	high	1342:1345	arg1	ratio					1361:1365	a high drug antibody ratio	1340:1365	a high drug antibody ratio based on IgG-ADC standards	1340:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	2	2	theme	sites	463:467	arg1	structure					419:427	polymeric structure	409:427	polymeric structure	409:427	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	2	2	theme	sites	463:467	arg1	number					439:444	large number	433:444	large number	433:444	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	2	2	theme	sites	463:467	arg1	weight					401:406	its high molecular weight	382:406	its high molecular weight	382:406	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	7	3	gly	glycopeptide	1301:1312	arg2	glycopeptide					1301:1312	glycopeptide	1301:1312	glycopeptide	1301:1312	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	6	4	theme	IgM	1092:1094	arg1	glycans					1096:1102	the IgM glycans	1088:1102	the IgM glycans	1088:1102	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	8	5	theme	expression	1491:1500	arg1	system					1502:1507	a mammalian cell expression system	1474:1507	a mammalian cell expression system	1474:1507	This platform methodology can be readily adapted for any human IgM produced in a mammalian cell expression system.					
31381874	7	6	theme	azide	1221:1225	arg1	incorporation					1227:1239	the azide incorporation	1217:1239	the azide incorporation	1217:1239	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	7	7	theme	antibody	1352:1359	arg1	ratio					1361:1365	a high drug antibody ratio	1340:1365	a high drug antibody ratio based on IgG-ADC standards	1340:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	4	8	theme	existing	658:665	arg1	methods					667:673	existing methods	658:673	existing methods for the development and characterization of IgG-ADCs	658:726	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	3	9	theme	next	534:537	arg1	generation					539:548	the next generation	530:548	the next generation protein biotherapeutic drugs	530:577	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	4	10	dep	development	683:693	arg1	the					679:681	the	679:681	the	679:681	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	6	11	theme	glycans	1174:1180	arg1	spectrometry					1131:1142	mass spectrometry	1126:1142	mass spectrometry of the enzymatically released glycans and glycopeptides	1126:1198	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	6	12	theme	released	1165:1172	arg1	glycans					1174:1180	glycans	1174:1180	glycans	1174:1180	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	3	13	dep	considered	519:528	arg1	isotype					646:652	the IgG isotype	638:652	the IgG isotype	638:652	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	7	14	theme	IgG-ADC	1376:1382	arg1	standards					1384:1392	IgG-ADC standards	1376:1392	IgG-ADC standards	1376:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	6	15	theme	glycopeptides	1186:1198	arg1	spectrometry					1131:1142	mass spectrometry	1126:1142	mass spectrometry of the enzymatically released glycans and glycopeptides	1126:1198	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	1	16	theme	complement	160:169	arg1	activation					171:180	complement activation	160:180	complement activation	160:180	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	6	17	gly	glycopeptides	1186:1198	arg2	glycopeptides					1186:1198	glycopeptides	1186:1198	glycopeptides	1186:1198	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	0	18	theme	glycan	15:20	arg1	labelling					22:30	Chemoenzymatic glycan labelling	0:30	Chemoenzymatic glycan labelling	0:30	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	4	19	theme	chemoenzymatic	839:852	arg1	method					854:859	a chemoenzymatic method	837:859	a chemoenzymatic method targeting the glycans on the IgM	837:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	7	20	theme	azide	1251:1255	arg1	ratio					1266:1270	an azide antibody ratio	1248:1270	an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards	1248:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	1	21	theme	Immunoglobulin	94:107	arg1	M					109:109	Immunoglobulin M	94:109	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	1	21	theme	Immunoglobulin	94:107	arg1	IgM					112:114	IgM	112:114	IgM	112:114	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	6	22	dep	glycans	1174:1180	arg1	the					1147:1149	the	1147:1149	the	1147:1149	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	0	23	theme	Chemoenzymatic	0:13	arg1	labelling					22:30	Chemoenzymatic glycan labelling	0:30	Chemoenzymatic glycan labelling	0:30	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	1	24	theme	M	109:109	arg1	antibodies					122:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	1	25	theme	cellular	183:190	arg1	clearance					199:207	cellular debris clearance	183:207	cellular debris clearance	183:207	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	2	26	theme	polymeric	409:417	arg1	structure					419:427	polymeric structure	409:427	polymeric structure	409:427	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	7	27	theme	8	1275:1275	arg1	ratio					1266:1270	an azide antibody ratio	1248:1270	an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards	1248:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	3	28	theme	protein	550:556	arg1	drugs					573:577	protein biotherapeutic drugs	550:577	the next generation protein biotherapeutic drugs	530:577	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	8	29	theme	platform	1400:1407	arg1	methodology					1409:1419	This platform methodology	1395:1419	This platform methodology	1395:1419	This platform methodology can be readily adapted for any human IgM produced in a mammalian cell expression system.					
31381874	1	30	theme	debris	192:197	arg1	clearance					199:207	cellular debris clearance	183:207	cellular debris clearance	183:207	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	4	31	theme	IgG-ADCs	719:726	arg1	characterization					699:714	characterization	699:714	characterization	699:714	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	4	31	theme	IgG-ADCs	719:726	arg1	development					683:693	development	683:693	development	683:693	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	3	32	theme	biotherapeutic	558:571	arg1	drugs					573:577	protein biotherapeutic drugs	550:577	the next generation protein biotherapeutic drugs	530:577	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	2	33	theme	molecular	391:399	arg1	weight					401:406	its high molecular weight	382:406	its high molecular weight	382:406	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	2	34	theme	glycosylation	449:461	arg1	sites					463:467	glycosylation sites	449:467	glycosylation sites	449:467	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	5	35	theme	functionalized	901:914	arg1	acids					923:927	Azide functionalized sialic acids	895:927	Azide functionalized sialic acids	895:927	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	7	36	theme	6-10	1295:1298	arg1	ratio					1266:1270	an azide antibody ratio	1248:1270	an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards	1248:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	5	37	theme	click	1025:1029	arg1	chemistry					1032:1040	"click" chemistry	1024:1040	"click" chemistry	1024:1040	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	6	38	theme	groups	1063:1068	arg1	number					1047:1052	The number	1043:1052	The number of azide groups incorporated onto the IgM glycans	1043:1102	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	2	39	theme	high	386:389	arg1	weight					401:406	its high molecular weight	382:406	its high molecular weight	382:406	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	1	40	theme	therapeutic	274:284	arg1	antibody					308:315	a therapeutic or targeting/delivery antibody	272:315	a therapeutic or targeting/delivery antibody	272:315	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	8	41	theme	human	1452:1456	arg1	IgM					1458:1460	any human IgM	1448:1460	any human IgM produced in a mammalian cell expression system	1448:1507	This platform methodology can be readily adapted for any human IgM produced in a mammalian cell expression system.					
31381874	2	42	gly	glycosylation	449:461	arg2	sites					463:467	glycosylation sites	449:467	glycosylation sites	449:467	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	1	43	theme	cell	213:216	arg1	control					226:232	cell quality control	213:232	cell quality control	213:232	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	0	44	theme	site-specific	50:62	arg1	conjugates					82:91	site-specific IgM-antibody drug conjugates	50:91	site-specific IgM-antibody drug conjugates	50:91	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	4	45	with	compatible	736:745	arg1	IgM-ADC					752:758	IgM-ADC	752:758	IgM-ADC	752:758	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	7	46	theme	antibody	1257:1264	arg1	ratio					1266:1270	an azide antibody ratio	1248:1270	an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards	1248:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	4	47	theme	specific	814:821	arg1	IgM-ADC					823:829	site specific IgM-ADC	809:829	site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM	809:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	3	48	theme	Site-specific	470:482	arg1	ADC					510:512	ADC	510:512	ADC	510:512	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	3	48	theme	Site-specific	470:482	arg1	antibody-drug-conjugates					484:507	Site-specific antibody-drug-conjugates	470:507	Site-specific antibody-drug-conjugates (ADC)	470:513	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	2	49	theme	large	433:437	arg1	number					439:444	large number	433:444	large number	433:444	However, this potential has not been explored thoroughly due to its high molecular weight, polymeric structure and large number of glycosylation sites.					
31381874	1	50	theme	targeting/delivery	289:306	arg1	antibody					308:315	a therapeutic or targeting/delivery antibody	272:315	a therapeutic or targeting/delivery antibody	272:315	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	8	51	theme	cell	1486:1489	arg1	system					1502:1507	a mammalian cell expression system	1474:1507	a mammalian cell expression system	1474:1507	This platform methodology can be readily adapted for any human IgM produced in a mammalian cell expression system.					
31381874	4	52	theme	site	809:812	arg1	IgM-ADC					823:829	site specific IgM-ADC	809:829	site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM	809:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	1	53	theme	type	117:120	arg1	antibodies					122:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	4	54	theme	platform	775:782	arg1	methodology					784:794	a platform methodology	773:794	a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM	773:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	5	55	theme	"	1030:1030	arg1	chemistry					1032:1040	"click" chemistry	1024:1040	"click" chemistry	1024:1040	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	7	56	theme	drug	1347:1350	arg1	ratio					1361:1365	a high drug antibody ratio	1340:1365	a high drug antibody ratio based on IgG-ADC standards	1340:1392	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	5	57	theme	biocompatible	992:1004	arg1	conjugation					1006:1016	biocompatible conjugation	992:1016	biocompatible conjugation using "click" chemistry	992:1040	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	4	58	theme	suitable	796:803	arg1	methodology					784:794	a platform methodology	773:794	a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM	773:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	5	59	theme	sialic	916:921	arg1	acids					923:927	Azide functionalized sialic acids	895:927	Azide functionalized sialic acids	895:927	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	1	60	theme	quality	218:224	arg1	control					226:232	cell quality control	213:232	cell quality control	213:232	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	3	61	theme	IgG	642:644	arg1	isotype					646:652	the IgG isotype	638:652	the IgG isotype	638:652	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	0	62	dep	labelling	22:30	arg1	platform					37:44	a platform	35:44	a platform for site-specific IgM-antibody drug conjugates	35:91	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	3	63	dep	generation	539:548	arg1	drugs					573:577	protein biotherapeutic drugs	550:577	the next generation protein biotherapeutic drugs	530:577	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	6	64	theme	mass	1126:1129	arg1	spectrometry					1131:1142	mass spectrometry	1126:1142	mass spectrometry of the enzymatically released glycans and glycopeptides	1126:1198	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	0	65	theme	drug	77:80	arg1	conjugates					82:91	site-specific IgM-antibody drug conjugates	50:91	site-specific IgM-antibody drug conjugates	50:91	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	3	66	theme	clinical	601:608	arg1	trials					610:615	clinical trials	601:615	clinical trials	601:615	Site-specific antibody-drug-conjugates (ADC) are considered the next generation protein biotherapeutic drugs and currently all, in clinical trials and approved, are of the IgG isotype.					
31381874	6	67	theme	azide	1057:1061	arg1	groups					1063:1068	azide groups	1057:1068	azide groups incorporated onto the IgM glycans	1057:1102	The number of azide groups incorporated onto the IgM glycans were characterized by mass spectrometry of the enzymatically released glycans and glycopeptides.					
31381874	5	68	theme	IgM	952:954	arg1	glycans					956:962	IgM glycans	952:962	IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry	952:1040	Azide functionalized sialic acids were incorporated onto IgM glycans using sialyltransferase for biocompatible conjugation using "click" chemistry.					
31381874	7	69	theme	glycopeptide	1301:1312	arg1	6-10					1295:1298	6-10	1295:1298	6-10	1295:1298	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	7	69	theme	glycopeptide	1301:1312	arg1	data					1314:1317	glycopeptide data	1301:1317	glycopeptide data	1301:1317	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	0	70	theme	IgM-antibody	64:75	arg1	conjugates					82:91	site-specific IgM-antibody drug conjugates	50:91	site-specific IgM-antibody drug conjugates	50:91	Chemoenzymatic glycan labelling as a platform for site-specific IgM-antibody drug conjugates.					
31381874	4	71	from	glycans	875:881	arg1	IgM					890:892	the IgM	886:892	the IgM	886:892	As existing methods for the development and characterization of IgG-ADCs are not compatible with IgM-ADC, we describe a platform methodology suitable for site specific IgM-ADC using a chemoenzymatic method targeting the glycans on the IgM.					
31381874	1	72	contain	have	239:242	arg1	antibodies					122:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies	94:131	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	1	72	contain	have	239:242	arg2	potential					248:256	the potential to be used as a therapeutic or targeting/delivery antibody	244:315	the potential to be used as a therapeutic or targeting/delivery antibody	244:315	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	7	73	theme	glycan	1278:1283	arg1	8					1275:1275	8	1275:1275	8	1275:1275	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	7	73	theme	glycan	1278:1283	arg1	data					1285:1288	glycan data	1278:1288	glycan data	1278:1288	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
31381874	1	74	theme	significant	140:150	arg1	role					152:155	a significant role	138:155	a significant role	138:155	Immunoglobulin M (IgM) type antibodies play a significant role in complement activation, cellular debris clearance and cell quality control, and have the potential to be used as a therapeutic or targeting/delivery antibody.					
31381874	7	75	theme	incorporation	1227:1239	arg1	Quantitation					1201:1212	Quantitation	1201:1212	Quantitation of the azide incorporation	1201:1239	Quantitation of the azide incorporation showed an azide antibody ratio of 8 (glycan data) and 6-10 (glycopeptide data) which translates to a high drug antibody ratio based on IgG-ADC standards.					
29299842	1	0	gly	glycosylation	77:89	arg1	protein					167:173	protein secretion	167:183	protein secretion	167:183	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	5	1	theme	clinical	808:815	arg1	implications					817:828	their biotechnological and clinical implications	781:828	their biotechnological and clinical implications	781:828	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	5	2	theme	glycosylation	661:673	arg1	understanding					636:648	a comprehensive understanding	620:648	a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics	620:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	8	3	theme	profiles	1242:1249	arg1	generation					1192:1201	the generation	1188:1201	the generation of species-and strain-specific glycome profiles	1188:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	3	theme	profiles	1242:1249	arg1	delineation					1259:1269	the delineation	1255:1269	the delineation of modified glycan structures in mutant and engineered yeast cells	1255:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	5	4	from	characteristics	730:744	arg1	understanding					636:648	a comprehensive understanding	620:648	a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics	620:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	8	5	from	generation	1192:1201	arg1	cells					1332:1336	engineered yeast cells	1315:1336	engineered yeast cells	1315:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	5	from	generation	1192:1201	arg1	mutant					1304:1309	mutant	1304:1309	mutant	1304:1309	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	6	6	theme	glycoproteins	984:996	arg1	production					958:967	the production	954:967	the production of recombinant glycoproteins with therapeutic potential	954:1023	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	8	7	from	structures	1290:1299	arg1	cells					1332:1336	engineered yeast cells	1315:1336	engineered yeast cells	1315:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	7	from	structures	1290:1299	arg1	mutant					1304:1309	mutant	1304:1309	mutant	1304:1309	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	7	8	theme	pathogenic	1061:1070	arg1	yeasts					1072:1077	pathogenic yeasts	1061:1077	pathogenic yeasts	1061:1077	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	1	9	theme	protein	167:173	arg1	secretion					175:183	protein secretion	167:183	protein secretion	167:183	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	8	10	from	cells	1332:1336	arg1	generation					1192:1201	the generation	1188:1201	the generation of species-and strain-specific glycome profiles	1188:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	10	from	cells	1332:1336	arg1	delineation					1259:1269	the delineation	1255:1269	the delineation of modified glycan structures in mutant and engineered yeast cells	1255:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	2	11	attach	attached	243:250	arg1	proteins					255:262	proteins	255:262	proteins	255:262	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	2	11	attach	attached	243:250	arg2	glycans					235:241	glycans	235:241	glycans attached to proteins	235:262	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	5	12	theme	useful	758:763	arg1	information					765:775	useful information	758:775	useful information for their biotechnological and clinical implications	758:828	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	6	13	theme	recombinant	972:982	arg1	glycoproteins					984:996	recombinant glycoproteins	972:996	recombinant glycoproteins	972:996	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	5	14	theme	various	678:684	arg1	species					692:698	various yeast species	678:698	various yeast species	678:698	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	7	15	theme	yeasts	1072:1077	arg1	targets					1107:1113	novel targets	1101:1113	novel targets for antifungal agents	1101:1135	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	7	15	theme	yeasts	1072:1077	arg1	glycans					1050:1056	The virulenceassociated glycans	1026:1056	The virulenceassociated glycans of pathogenic yeasts	1026:1077	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	6	16	gly	glycoproteins	984:996	arg1	glycoproteins					984:996	recombinant glycoproteins	972:996	recombinant glycoproteins	972:996	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	8	17	theme	glycan	1283:1288	arg1	structures					1290:1299	modified glycan structures	1274:1299	modified glycan structures in mutant and engineered yeast cells	1274:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	5	18	theme	yeast	686:690	arg1	species					692:698	various yeast species	678:698	various yeast species	678:698	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	2	19	theme	different	288:296	arg1	organisms					298:306	different organisms	288:306	different organisms	288:306	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	4	20	theme	host	602:605	arg1	cells					607:611	host cells	602:611	host cells	602:611	Moreover, in pathogenic yeasts, glycans assembled on cell-surface glycoproteins can mediate their interactions with host cells.					
29299842	0	21	from	proteins	49:56	arg1	yeasts					61:66	yeasts	61:66	yeasts	61:66	Structural analysis of N-/O-glycans assembled on proteins in yeasts.					
29299842	5	22	theme	comprehensive	622:634	arg1	understanding					636:648	a comprehensive understanding	620:648	a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics	620:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	1	23	theme	Protein	69:75	arg1	glycosylation					77:89	Protein glycosylation	69:89	Protein glycosylation	69:89	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	1	23	theme	Protein	69:75	arg1	modification					142:153	the most universal and diverse post-translational modification	92:153	the most universal and diverse post-translational modification	92:153	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	3	24	theme	protein	349:355	arg1	glycosylation					357:369	protein glycosylation	349:369	protein glycosylation	349:369	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	8	25	theme	several	1148:1154	arg1	techniques					1166:1175	Nowadays, several glycomics techniques	1138:1175	techniques	1166:1175	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	6	26	from	pathways	928:935	arg1	yeast					940:944	yeast	940:944	yeast	940:944	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	9	27	theme	several	1505:1511	arg1	species					1519:1525	several yeast species	1505:1525	several yeast species	1505:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	5	28	gly	glycosylation	661:673	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	5	28	gly	glycosylation	661:673	arg1	species					692:698	various yeast species	678:698	various yeast species	678:698	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	8	29	theme	engineered	1315:1324	arg1	cells					1332:1336	engineered yeast cells	1315:1336	engineered yeast cells	1315:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	9	30	theme	wall	1483:1486	arg1	mannoproteins					1488:1500	cell wall mannoproteins	1478:1500	cell wall mannoproteins	1478:1500	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	8	31	theme	Nowadays	1138:1145	arg1	techniques					1166:1175	Nowadays, several glycomics techniques	1138:1175	techniques	1166:1175	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	7	32	theme	virulenceassociated	1030:1048	arg1	targets					1107:1113	novel targets	1101:1113	novel targets for antifungal agents	1101:1135	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	7	32	theme	virulenceassociated	1030:1048	arg1	glycans					1050:1056	The virulenceassociated glycans	1026:1056	The virulenceassociated glycans of pathogenic yeasts	1026:1077	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	2	33	theme	yeast	324:328	arg1	species					330:336	yeast species	324:336	yeast species	324:336	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	8	34	theme	strain-specific	1218:1232	arg1	profiles					1242:1249	species-and strain-specific glycome profiles	1206:1249	species-and strain-specific glycome profiles	1206:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	3	35	theme	proteins	423:430	arg1	control					402:408	the quality control	390:408	the quality control of secretory proteins	390:430	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	0	36	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of N-/O-glycans	0:34	Structural analysis of N-/O-glycans assembled on proteins in yeasts.					
29299842	0	37	theme	N-/O-glycans	23:34	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of N-/O-glycans	0:34	Structural analysis of N-/O-glycans assembled on proteins in yeasts.					
29299842	5	38	from	glycosylation	661:673	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	5	38	from	glycosylation	661:673	arg1	species					692:698	various yeast species	678:698	various yeast species	678:698	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	8	39	theme	glycomics	1156:1164	arg1	techniques					1166:1175	Nowadays, several glycomics techniques	1138:1175	techniques	1166:1175	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	40	theme	modified	1274:1281	arg1	structures					1290:1299	modified glycan structures	1274:1299	modified glycan structures in mutant and engineered yeast cells	1274:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	3	41	theme	key	377:379	arg1	roles					381:385	key roles	377:385	key roles	377:385	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	6	42	theme	Yeast-specific	831:844	arg1	glycans					846:852	Yeast-specific glycans	831:852	Yeast-specific glycans	831:852	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	6	42	theme	Yeast-specific	831:844	arg1	target					860:865	a target	858:865	a target for glyco-engineering	858:887	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	5	43	theme	structure	720:728	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	3	44	theme	cell	465:468	arg1	integrity					475:483	cell wall integrity	465:483	cell wall integrity	465:483	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	8	45	theme	yeast	1326:1330	arg1	cells					1332:1336	engineered yeast cells	1315:1336	engineered yeast cells	1315:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	46	from	mutant	1304:1309	arg1	generation					1192:1201	the generation	1188:1201	the generation of species-and strain-specific glycome profiles	1188:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	46	from	mutant	1304:1309	arg1	delineation					1259:1269	the delineation	1255:1269	the delineation of modified glycan structures in mutant and engineered yeast cells	1255:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	1	47	theme	universal	101:109	arg1	modification					142:153	the most universal and diverse post-translational modification	92:153	the most universal and diverse post-translational modification	92:153	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	1	47	theme	universal	101:109	arg1	glycosylation					77:89	Protein glycosylation	69:89	Protein glycosylation	69:89	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	3	48	theme	wall	470:473	arg1	integrity					475:483	cell wall integrity	465:483	cell wall integrity	465:483	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	5	49	from	understanding	636:648	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	5	49	from	understanding	636:648	arg1	species					692:698	various yeast species	678:698	various yeast species	678:698	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	8	50	theme	species-and	1206:1216	arg1	profiles					1242:1249	species-and strain-specific glycome profiles	1206:1249	species-and strain-specific glycome profiles	1206:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	6	51	with	production	958:967	arg1	potential					1015:1023	therapeutic potential	1003:1023	therapeutic potential	1003:1023	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	9	52	theme	cell	1478:1481	arg1	mannoproteins					1488:1500	cell wall mannoproteins	1478:1500	cell wall mannoproteins	1478:1500	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	3	53	theme	secretory	413:421	arg1	proteins					423:430	secretory proteins	413:430	secretory proteins	413:430	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	8	54	from	delineation	1259:1269	arg1	cells					1332:1336	engineered yeast cells	1315:1336	engineered yeast cells	1315:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	54	from	delineation	1259:1269	arg1	mutant					1304:1309	mutant	1304:1309	mutant	1304:1309	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	2	55	theme	glycans	235:241	arg1	structures					221:230	The structures	217:230	The structures of glycans attached to proteins	217:262	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	2	55	theme	glycans	235:241	arg1	diverse					274:280	diverse	274:280	diverse	274:280	The structures of glycans attached to proteins are quite diverse among different organisms and even within yeast species.					
29299842	4	56	gly	glycoproteins	552:564	arg1	glycoproteins					552:564	cell-surface glycoproteins	539:564	cell-surface glycoproteins	539:564	Moreover, in pathogenic yeasts, glycans assembled on cell-surface glycoproteins can mediate their interactions with host cells.					
29299842	3	57	theme	quality	394:400	arg1	control					402:408	the quality control	390:408	the quality control of secretory proteins	390:430	In yeast, protein glycosylation plays key roles in the quality control of secretory proteins, and particularly in maintaining cell wall integrity.					
29299842	8	58	theme	structures	1290:1299	arg1	generation					1192:1201	the generation	1188:1201	the generation of species-and strain-specific glycome profiles	1188:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	58	theme	structures	1290:1299	arg1	delineation					1259:1269	the delineation	1255:1269	the delineation of modified glycan structures in mutant and engineered yeast cells	1255:1336	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	8	59	theme	glycome	1234:1240	arg1	profiles					1242:1249	species-and strain-specific glycome profiles	1206:1249	species-and strain-specific glycome profiles	1206:1249	Nowadays, several glycomics techniques facilitate the generation of species-and strain-specific glycome profiles and the delineation of modified glycan structures in mutant and engineered yeast cells.					
29299842	7	60	theme	antifungal	1119:1128	arg1	agents					1130:1135	antifungal agents	1119:1135	antifungal agents	1119:1135	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	9	61	theme	O-glycan	1422:1429	arg1	chains					1431:1436	the N-and O-glycan chains	1412:1436	the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species	1412:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	4	62	theme	cell-surface	539:550	arg1	glycoproteins					552:564	cell-surface glycoproteins	539:564	cell-surface glycoproteins	539:564	Moreover, in pathogenic yeasts, glycans assembled on cell-surface glycoproteins can mediate their interactions with host cells.					
29299842	6	63	theme	therapeutic	1003:1013	arg1	potential					1015:1023	therapeutic potential	1003:1023	therapeutic potential	1003:1023	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	6	64	theme	glycosylation	914:926	arg1	pathways					928:935	implementing human-type glycosylation pathways	890:935	implementing human-type glycosylation pathways in yeast	890:944	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	7	65	theme	novel	1101:1105	arg1	targets					1107:1113	novel targets	1101:1113	novel targets for antifungal agents	1101:1135	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	7	65	theme	novel	1101:1105	arg1	glycans					1050:1056	The virulenceassociated glycans	1026:1056	The virulenceassociated glycans of pathogenic yeasts	1026:1077	The virulenceassociated glycans of pathogenic yeasts could be exploited as novel targets for antifungal agents.					
29299842	1	66	theme	diverse	115:121	arg1	modification					142:153	the most universal and diverse post-translational modification	92:153	the most universal and diverse post-translational modification	92:153	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	1	66	theme	diverse	115:121	arg1	glycosylation					77:89	Protein glycosylation	69:89	Protein glycosylation	69:89	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	9	67	theme	yeast	1513:1517	arg1	species					1519:1525	several yeast species	1505:1525	several yeast species	1505:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	5	68	theme	defining	704:711	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	6	69	theme	human-type	903:912	arg1	pathways					928:935	implementing human-type glycosylation pathways	890:935	implementing human-type glycosylation pathways in yeast	890:944	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	9	70	gly	glycoproteins	1461:1473	arg1	glycoproteins					1461:1473	glycoproteins	1461:1473	glycoproteins	1461:1473	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	9	71	attach	released	1438:1445	arg1	glycoproteins					1461:1473	glycoproteins	1461:1473	glycoproteins	1461:1473	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	9	71	attach	released	1438:1445	arg2	chains					1431:1436	the N-and O-glycan chains	1412:1436	the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species	1412:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	9	71	attach	released	1438:1445	arg1	mannoproteins					1488:1500	cell wall mannoproteins	1478:1500	cell wall mannoproteins	1478:1500	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	1	72	theme	post-translational	123:140	arg1	modification					142:153	the most universal and diverse post-translational modification	92:153	the most universal and diverse post-translational modification	92:153	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	1	72	theme	post-translational	123:140	arg1	glycosylation					77:89	Protein glycosylation	69:89	Protein glycosylation	69:89	Protein glycosylation, the most universal and diverse post-translational modification, can affect protein secretion, stability, and immunogenicity.					
29299842	5	73	theme	glycan	713:718	arg1	characteristics					730:744	defining glycan structure characteristics	704:744	defining glycan structure characteristics	704:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	6	74	theme	implementing	890:901	arg1	pathways					928:935	implementing human-type glycosylation pathways	890:935	implementing human-type glycosylation pathways in yeast	890:944	Yeast-specific glycans are a target for glyco-engineering; implementing human-type glycosylation pathways in yeast can aid the production of recombinant glycoproteins with therapeutic potential.					
29299842	9	75	from	glycoproteins	1461:1473	arg1	species					1519:1525	several yeast species	1505:1525	several yeast species	1505:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	5	76	theme	biotechnological	787:802	arg1	implications					817:828	their biotechnological and clinical implications	781:828	their biotechnological and clinical implications	781:828	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	9	77	theme	purified	1452:1459	arg1	glycoproteins					1461:1473	glycoproteins	1461:1473	glycoproteins	1461:1473	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	9	78	theme	N-and	1416:1420	arg1	chains					1431:1436	the N-and O-glycan chains	1412:1436	the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species	1412:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
29299842	5	79	from	species	692:698	arg1	understanding					636:648	a comprehensive understanding	620:648	a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics	620:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	4	80	theme	pathogenic	499:508	arg1	yeasts					510:515	pathogenic yeasts	499:515	pathogenic yeasts	499:515	Moreover, in pathogenic yeasts, glycans assembled on cell-surface glycoproteins can mediate their interactions with host cells.					
29299842	4	81	with	interactions	584:595	arg1	cells					607:611	host cells	602:611	host cells	602:611	Moreover, in pathogenic yeasts, glycans assembled on cell-surface glycoproteins can mediate their interactions with host cells.					
29299842	5	82	theme	protein	653:659	arg1	glycosylation					661:673	protein glycosylation	653:673	protein glycosylation in various yeast species and defining glycan structure characteristics	653:744	Thus, a comprehensive understanding of protein glycosylation in various yeast species and defining glycan structure characteristics can provide useful information for their biotechnological and clinical implications.					
29299842	9	83	from	mannoproteins	1488:1500	arg1	species					1519:1525	several yeast species	1505:1525	several yeast species	1505:1525	Here, we present the protocols employed in our laboratory to investigate the N-and O-glycan chains released from purified glycoproteins or cell wall mannoproteins in several yeast species.					
31398379	3	0	from	larvae	1105:1110	arg1	pools					1089:1093	the anionic pools	1077:1093	the anionic pools from both larvae and venom	1077:1120	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	1	1	theme	agriculturally-	450:464	arg1	mellifera					436:444	the honeybee Apis mellifera	418:444	the honeybee Apis mellifera	418:444	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	1	1	theme	agriculturally-	450:464	arg1	species					506:512	an agriculturally- and allergologically-significant social species	447:512	an agriculturally- and allergologically-significant social species	447:512	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	2	2	theme	venom	729:733	arg1	N-glycans					682:690	the N-glycans	678:690	the N-glycans of male honeybee larvae and honeybee venom	678:733	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	2	3	theme	chemical	646:653	arg1	treatments					655:664	enzymatic and chemical treatments	632:664	enzymatic and chemical treatments	632:664	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	6	4	theme	oligosaccharide	1934:1948	arg1	structures					1950:1959	rather complex anionic oligosaccharide structures	1911:1959	rather complex anionic oligosaccharide structures	1911:1959	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	5	5	theme	specific	1660:1667	arg1	glycoproteins					1675:1687	specific venom glycoproteins	1660:1687	specific venom glycoproteins	1660:1687	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	1	6	theme	allergologically-significant	470:497	arg1	mellifera					436:444	the honeybee Apis mellifera	418:444	the honeybee Apis mellifera	418:444	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	1	6	theme	allergologically-significant	470:497	arg1	species					506:512	an agriculturally- and allergologically-significant social species	447:512	an agriculturally- and allergologically-significant social species	447:512	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	4	7	gly	fucosylated	1301:1311	arg1	glycans					1343:1349	more fucosylated and fewer Man4/5-based hybrid glycans	1296:1349	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae	1296:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	1	8	theme	species	189:195	arg1	comparisons					148:158	BACKGROUND Previous glycophylogenetic comparisons	110:158	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species	110:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	3	9	theme	wide	1134:1137	arg1	variety					1139:1145	a wide variety	1132:1145	a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1132:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	9	theme	wide	1134:1137	arg1	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	10	theme	neutral	805:811	arg1	N-glycome					820:828	The neutral larval N-glycome	801:828	RESULTS The neutral larval N-glycome	793:828	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	5	11	theme	royal	1520:1524	arg1	jelly					1526:1530	royal jelly	1520:1530	royal jelly	1520:1530	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	4	12	theme	Man4/5-based	1323:1334	arg1	glycans					1343:1349	more fucosylated and fewer Man4/5-based hybrid glycans	1296:1349	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae	1296:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	2	13	theme	spectrometry	589:600	arg1	approach					602:609	an off-line liquid chromatography/mass spectrometry approach	550:609	an off-line liquid chromatography/mass spectrometry approach	550:609	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	6	14	theme	Apis	1808:1811	arg1	mellifera					1813:1821	Apis mellifera	1808:1821	Apis mellifera	1808:1821	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	2	15	theme	enzymatic	632:640	arg1	treatments					655:664	enzymatic and chemical treatments	632:664	enzymatic and chemical treatments	632:664	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	6	16	theme	N-glycans	1795:1803	arg1	regions					1784:1790	the core and antennal regions	1762:1790	regions	1784:1790	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	1	17	theme	Apis	431:434	arg1	mellifera					436:444	the honeybee Apis mellifera	418:444	the honeybee Apis mellifera	418:444	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	1	17	theme	Apis	431:434	arg1	species					506:512	an agriculturally- and allergologically-significant social species	447:512	an agriculturally- and allergologically-significant social species	447:512	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	6	18	theme	SIGNIFICANCE	1690:1701	arg1	data					1707:1710	SIGNIFICANCE Our data	1690:1710	SIGNIFICANCE Our data	1690:1710	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	1	19	theme	BACKGROUND	110:119	arg1	comparisons					148:158	BACKGROUND Previous glycophylogenetic comparisons	110:158	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species	110:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	6	20	theme	regions	1784:1790	arg1	modification					1746:1757	tissue-specific modification	1730:1757	tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera	1730:1821	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	3	21	theme	paucimannosidic	867:881	arg1	structures					883:892	paucimannosidic structures	867:892	paucimannosidic structures	867:892	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	22	with	N-glycans	1209:1217	arg1	antennae					1236:1243	up to three antennae	1224:1243	up to three antennae	1224:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	23	theme	venom	913:917	arg1	N-glycome					919:927	the neutral venom N-glycome	901:927	the neutral venom N-glycome	901:927	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	2	24	theme	structures	781:790	arg1	definition					758:767	definition	758:767	definition of isomeric structures	758:790	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	3	25	dep	RESULTS	793:799	arg1	N-glycome					820:828	The neutral larval N-glycome	801:828	RESULTS The neutral larval N-glycome	793:828	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	4	26	theme	royal	1272:1276	arg1	jelly					1278:1282	honeybee royal jelly	1263:1282	honeybee royal jelly	1263:1282	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	3	27	dep	three	1230:1234	arg1	to					1227:1228	to	1227:1228	to	1227:1228	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	28	theme	core	1766:1769	arg1	regions					1784:1790	the core and antennal regions	1762:1790	regions	1784:1790	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	3	29	theme	processed	944:952	arg1	hybrid					954:959	more processed hybrid and complex forms	939:977	hybrid	954:959	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	2	30	theme	liquid	562:567	arg1	spectrometry					589:600	off-line liquid chromatography/mass spectrometry	553:600	an off-line liquid chromatography/mass spectrometry approach	550:609	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	5	31	theme	total	1535:1539	arg1	compositions					1584:1595	some 150 compositions	1575:1595	some 150 compositions	1575:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	5	31	theme	total	1535:1539	arg1	repertoire					1561:1570	a total honeybee N-glycomic repertoire	1533:1570	a total honeybee N-glycomic repertoire of some 150 compositions	1533:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	0	32	theme	mellifera	82:90	arg1	N-glycomes					63:72	the N-glycomes	59:72	the N-glycomes of Apis mellifera larvae and venom	59:107	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	2	33	dep	METHODS	515:521	arg1	employed					541:548	employed	541:548	employed	541:548	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	3	34	theme	antennal	984:991	arg1	residues					1037:1044	antennal N-acetylgalactosamine, galactose and fucose residues	984:1044	residues	1037:1044	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	34	theme	antennal	984:991	arg1	epitopes					1067:1074	Lewis-like epitopes	1056:1074	Lewis-like epitopes	1056:1074	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	1	35	theme	glycophylogenetic	130:146	arg1	comparisons					148:158	BACKGROUND Previous glycophylogenetic comparisons	110:158	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species	110:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	5	36	theme	N-glycomic	1550:1559	arg1	compositions					1584:1595	some 150 compositions	1575:1595	some 150 compositions	1575:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	5	36	theme	N-glycomic	1550:1559	arg1	repertoire					1561:1570	a total honeybee N-glycomic repertoire	1533:1570	a total honeybee N-glycomic repertoire of some 150 compositions	1533:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	1	37	dep	agriculturally-	450:464	arg1	social					499:504	social	499:504	social	499:504	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	3	38	theme	N-glycans	1209:1217	arg1	variety					1139:1145	a wide variety	1132:1145	a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1132:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	38	theme	N-glycans	1209:1217	arg1	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	39	theme	tissue-specific	1730:1744	arg1	modification					1746:1757	tissue-specific modification	1730:1757	tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera	1730:1821	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	0	40	dep	mellifera	82:90	arg1	larvae					92:97	larvae	92:97	larvae	92:97	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	3	41	contain	contained	1122:1130	arg2	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	41	contain	contained	1122:1130	arg2	variety					1139:1145	a wide variety	1132:1145	a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1132:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	41	contain	contained	1122:1130	arg1	pools					1089:1093	the anionic pools	1077:1093	the anionic pools from both larvae and venom	1077:1120	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	4	42	from	lengths	1415:1421	arg1	larvae					1358:1363	the larvae	1354:1363	the larvae	1354:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	4	43	theme	fucosylated	1301:1311	arg1	glycans					1343:1349	more fucosylated and fewer Man4/5-based hybrid glycans	1296:1349	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae	1296:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	0	44	theme	Tissue-specific	0:14	arg1	glycosylation					16:28	Tissue-specific glycosylation	0:28	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.	0:108	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	6	45	from	mellifera	1813:1821	arg1	regions					1784:1790	the core and antennal regions	1762:1790	regions	1784:1790	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	0	46	gly	glycosylation	16:28	arg1	honeybee					37:44	the honeybee	33:44	the honeybee	33:44	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	3	47	theme	Lewis-like	1056:1065	arg1	epitopes					1067:1074	Lewis-like epitopes	1056:1074	Lewis-like epitopes	1056:1074	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	48	theme	processing	1887:1896	arg1	capable					1866:1872	capable	1866:1872	capable	1866:1872	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	4	49	from	samples	1375:1381	arg1	larvae					1358:1363	the larvae	1354:1363	the larvae	1354:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	1	50	theme	glycomic	351:358	arg1	variations					360:369	order-specific glycomic variations	336:369	order-specific glycomic variations	336:369	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	1	51	theme	zwitterionic	236:247	arg1	modifications					249:261	the anionic and zwitterionic modifications	220:261	the anionic and zwitterionic modifications of their N-glycans	220:280	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	3	52	theme	anionic	1081:1087	arg1	pools					1089:1093	the anionic pools	1077:1093	the anionic pools from both larvae and venom	1077:1120	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	4	53	theme	venom	1369:1373	arg1	samples					1375:1381	venom samples	1369:1381	venom samples	1369:1381	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	1	54	theme	N-glycans	272:280	arg1	modifications					249:261	the anionic and zwitterionic modifications	220:261	the anionic and zwitterionic modifications of their N-glycans	220:280	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	6	55	theme	anionic	1926:1932	arg1	structures					1950:1959	rather complex anionic oligosaccharide structures	1911:1959	rather complex anionic oligosaccharide structures	1911:1959	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	5	56	theme	venom	1669:1673	arg1	glycoproteins					1675:1687	specific venom glycoproteins	1660:1687	specific venom glycoproteins	1660:1687	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	4	57	theme	antennal	1406:1413	arg1	lengths					1415:1421	contrasting antennal lengths	1394:1421	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths	1296:1421	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	5	58	theme	honeybee	1541:1548	arg1	compositions					1584:1595	some 150 compositions	1575:1595	some 150 compositions	1575:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	5	58	theme	honeybee	1541:1548	arg1	repertoire					1561:1570	a total honeybee N-glycomic repertoire	1533:1570	a total honeybee N-glycomic repertoire of some 150 compositions	1533:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	1	59	theme	lepidopteran	176:187	arg1	species					189:195	dipteran and lepidopteran species	163:195	species	189:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	3	60	theme	larval	813:818	arg1	N-glycome					820:828	The neutral larval N-glycome	801:828	RESULTS The neutral larval N-glycome	793:828	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	5	61	gly	glycoproteins	1675:1687	arg1	glycoproteins					1675:1687	specific venom glycoproteins	1660:1687	specific venom glycoproteins	1660:1687	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	1	62	theme	anionic	224:230	arg1	modifications					249:261	the anionic and zwitterionic modifications	220:261	the anionic and zwitterionic modifications of their N-glycans	220:280	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	2	63	theme	chromatography/mass	569:587	arg1	spectrometry					589:600	off-line liquid chromatography/mass spectrometry	553:600	an off-line liquid chromatography/mass spectrometry approach	550:609	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	1	64	theme	honeybee	422:429	arg1	mellifera					436:444	the honeybee Apis mellifera	418:444	the honeybee Apis mellifera	418:444	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	1	64	theme	honeybee	422:429	arg1	species					506:512	an agriculturally- and allergologically-significant social species	447:512	an agriculturally- and allergologically-significant social species	447:512	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	2	65	theme	honeybee	700:707	arg1	larvae					709:714	male honeybee larvae	695:714	male honeybee larvae	695:714	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	1	66	theme	Previous	121:128	arg1	comparisons					148:158	BACKGROUND Previous glycophylogenetic comparisons	110:158	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species	110:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	6	67	theme	antennal	1775:1782	arg1	regions					1784:1790	the core and antennal regions	1762:1790	regions	1784:1790	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	5	68	from	data	1458:1461	arg1	larvae					1476:1481	larvae	1476:1481	larvae	1476:1481	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	5	68	from	data	1458:1461	arg1	venom					1466:1470	venom	1466:1470	venom	1466:1470	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	4	69	theme	honeybee	1263:1270	arg1	jelly					1278:1282	honeybee royal jelly	1263:1282	honeybee royal jelly	1263:1282	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	0	70	theme	N-glycomes	63:72	arg1	Analysis					47:54	Analysis	47:54	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.	0:108	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	1	71	from	variations	206:215	arg1	modifications					249:261	the anionic and zwitterionic modifications	220:261	the anionic and zwitterionic modifications of their N-glycans	220:280	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	2	72	theme	isomeric	772:779	arg1	structures					781:790	isomeric structures	772:790	isomeric structures	772:790	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	0	73	theme	Apis	77:80	arg1	mellifera					82:90	Apis mellifera larvae and venom	77:107	mellifera	82:90	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	3	74	theme	glucuronylated	1150:1163	arg1	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	1	75	theme	dipteran	163:170	arg1	species					189:195	dipteran and lepidopteran species	163:195	species	189:195	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	3	76	theme	complex	965:971	arg1	forms					973:977	more processed hybrid and complex forms	939:977	forms	973:977	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	0	77	from	glycosylation	16:28	arg1	honeybee					37:44	the honeybee	33:44	the honeybee	33:44	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	5	78	theme	compositions	1584:1595	arg1	compositions					1584:1595	some 150 compositions	1575:1595	some 150 compositions	1575:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	5	78	theme	compositions	1584:1595	arg1	repertoire					1561:1570	a total honeybee N-glycomic repertoire	1533:1570	a total honeybee N-glycomic repertoire of some 150 compositions	1533:1595	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	2	79	theme	larvae	709:714	arg1	N-glycans					682:690	the N-glycans	678:690	the N-glycans of male honeybee larvae and honeybee venom	678:733	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	3	80	theme	neutral	905:911	arg1	N-glycome					919:927	the neutral venom N-glycome	901:927	the neutral venom N-glycome	901:927	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	2	81	theme	off-line	553:560	arg1	spectrometry					589:600	off-line liquid chromatography/mass spectrometry	553:600	an off-line liquid chromatography/mass spectrometry approach	550:609	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	2	82	theme	male	695:698	arg1	larvae					709:714	male honeybee larvae	695:714	male honeybee larvae	695:714	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	0	83	theme	venom	103:107	arg1	N-glycomes					63:72	the N-glycomes	59:72	the N-glycomes of Apis mellifera larvae and venom	59:107	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	3	84	theme	phosphoethanolamine-modified	1180:1207	arg1	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	85	theme	modification	1746:1757	arg1	indicative					1716:1725	indicative	1716:1725	indicative	1716:1725	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	4	86	from	glycans	1343:1349	arg1	larvae					1358:1363	the larvae	1354:1363	the larvae	1354:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	3	87	theme	N-acetylgalactosamine	993:1013	arg1	residues					1037:1044	antennal N-acetylgalactosamine, galactose and fucose residues	984:1044	residues	1037:1044	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	87	theme	N-acetylgalactosamine	993:1013	arg1	epitopes					1067:1074	Lewis-like epitopes	1056:1074	Lewis-like epitopes	1056:1074	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	5	88	theme	current	1450:1456	arg1	data					1458:1461	the current data	1446:1461	the current data on venom and larvae	1446:1481	CONCLUSIONS Combining the current data on venom and larvae with that we previously published on royal jelly, a total honeybee N-glycomic repertoire of some 150 compositions can be proposed in addition to the 20 previously identified on specific venom glycoproteins.					
31398379	3	89	theme	sulphated	1166:1174	arg1	N-glycans					1209:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans	1150:1217	glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae	1150:1243	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	90	from	N-glycans	1795:1803	arg1	mellifera					1813:1821	Apis mellifera	1808:1821	Apis mellifera	1808:1821	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	6	91	theme	complex	1918:1924	arg1	structures					1950:1959	rather complex anionic oligosaccharide structures	1911:1959	rather complex anionic oligosaccharide structures	1911:1959	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	2	92	theme	honeybee	720:727	arg1	venom					729:733	honeybee venom	720:733	honeybee venom	720:733	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
31398379	3	93	theme	fucose	1030:1035	arg1	residues					1037:1044	antennal N-acetylgalactosamine, galactose and fucose residues	984:1044	residues	1037:1044	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	93	theme	fucose	1030:1035	arg1	epitopes					1067:1074	Lewis-like epitopes	1056:1074	Lewis-like epitopes	1056:1074	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	94	theme	galactose	1016:1024	arg1	residues					1037:1044	antennal N-acetylgalactosamine, galactose and fucose residues	984:1044	residues	1037:1044	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	3	94	theme	galactose	1016:1024	arg1	epitopes					1067:1074	Lewis-like epitopes	1056:1074	Lewis-like epitopes	1056:1074	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	4	95	theme	contrasting	1394:1404	arg1	lengths					1415:1421	contrasting antennal lengths	1394:1421	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths	1296:1421	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	3	96	from	venom	1116:1120	arg1	pools					1089:1093	the anionic pools	1077:1093	the anionic pools from both larvae and venom	1077:1120	RESULTS The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic structures, while the neutral venom N-glycome displayed more processed hybrid and complex forms with antennal N-acetylgalactosamine, galactose and fucose residues including Lewis-like epitopes; the anionic pools from both larvae and venom contained a wide variety of glucuronylated, sulphated and phosphoethanolamine-modified N-glycans with up to three antennae.					
31398379	6	97	from	regions	1784:1790	arg1	mellifera					1813:1821	Apis mellifera	1808:1821	Apis mellifera	1808:1821	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	6	98	theme	extensive	1877:1885	arg1	processing					1887:1896	extensive processing	1877:1896	extensive processing to result in rather complex anionic oligosaccharide structures	1877:1959	SIGNIFICANCE Our data are indicative of tissue-specific modification of the core and antennal regions of N-glycans in Apis mellifera and reinforce the concept that insects are capable of extensive processing to result in rather complex anionic oligosaccharide structures.					
31398379	4	99	dep	Man4/5-based	1323:1334	arg1	fewer					1317:1321	fewer	1317:1321	fewer	1317:1321	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	1	100	theme	order-specific	336:349	arg1	variations					360:369	order-specific glycomic variations	336:369	order-specific glycomic variations	336:369	BACKGROUND Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.					
31398379	0	101	dep	glycosylation	16:28	arg1	Analysis					47:54	Analysis	47:54	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.	0:108	Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.					
31398379	4	102	theme	hybrid	1336:1341	arg1	glycans					1343:1349	more fucosylated and fewer Man4/5-based hybrid glycans	1296:1349	more fucosylated and fewer Man4/5-based hybrid glycans in the larvae	1296:1363	In comparison to honeybee royal jelly, there were more fucosylated and fewer Man4/5-based hybrid glycans in the larvae and venom samples as well as contrasting antennal lengths.					
31398379	2	103	with	combination	615:625	arg1	treatments					655:664	enzymatic and chemical treatments	632:664	enzymatic and chemical treatments	632:664	METHODS In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.					
30224661	5	0	from	Golgi	980:984	arg1	addition					944:951	the addition	940:951	the addition of the first GlcNAc in the Golgi	940:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	1	1	theme	sugars	166:171	arg1	form					147:150	the form	143:150	the form of nucleotide sugars	143:171	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	7	2	theme	GIPC	1348:1351	arg1	profiles					1353:1360	N-glycan or GIPC profiles	1336:1360	N-glycan or GIPC profiles	1336:1360	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
30224661	5	3	from	alleles	864:870	arg1	population					842:851	the N-glycopeptide population	823:851	the N-glycopeptide population from these alleles	823:870	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	3	4	dep	it	631:632	arg1	UGNT1					660:664	UGNT1	660:664	UGNT1	660:664	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	3	4	dep	it	631:632	arg1	transporter					645:655	UDP-GlcNAc transporter 1	634:657	it UDP-GlcNAc transporter 1 (UGNT1)	631:665	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	8	5	theme	GIPC	1512:1515	arg1	class					1517:1521	the GIPC class	1508:1521	the GIPC class of sphingolipids	1508:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	3	6	theme	UDP-GlcNAc	634:643	arg1	UGNT1					660:664	UGNT1	660:664	UGNT1	660:664	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	3	6	theme	UDP-GlcNAc	634:643	arg1	transporter					645:655	UDP-GlcNAc transporter 1	634:657	it UDP-GlcNAc transporter 1 (UGNT1)	631:665	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	7	7	from	changes	1325:1331	arg1	profiles					1353:1360	N-glycan or GIPC profiles	1336:1360	N-glycan or GIPC profiles	1336:1360	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
30224661	8	8	contain	contain	1395:1401	arg2	transporter					1423:1433	a single UDP-GlcNAc transporter	1403:1433	a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids	1403:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	8	8	contain	contain	1395:1401	arg1	plants					1388:1393	plants	1388:1393	plants	1388:1393	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	5	9	gly	N-glycopeptide	827:840	arg2	N-glycopeptide					827:840	N-glycopeptide	827:840	N-glycopeptide	827:840	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	4	10	theme	hybrid	796:801	arg1	N-glycans					803:811	hybrid N-glycans	796:811	hybrid N-glycans	796:811	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	2	11	theme	nucleotide	442:451	arg1	transporters					459:470	nucleotide sugar transporters	442:470	nucleotide sugar transporters	442:470	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	8	12	theme	N-glycans	1494:1502	arg1	maturation					1480:1489	the maturation	1476:1489	the maturation of N-glycans	1476:1502	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	8	12	theme	N-glycans	1494:1502	arg1	class					1517:1521	the GIPC class	1508:1521	the GIPC class of sphingolipids	1508:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	2	13	theme	Golgi	427:431	arg1	lumen					433:437	the Golgi lumen	423:437	the Golgi lumen	423:437	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	4	14	gly	N-glycopeptides	680:694	arg2	N-glycopeptides					680:694	N-glycopeptides	680:694	N-glycopeptides	680:694	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	0	15	link	N-linked	55:62	arg1	glycans					64:70	N-linked glycans	55:70	N-linked glycans	55:70	A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids.					
30224661	5	16	theme	first	960:964	arg1	GlcNAc					966:971	the first GlcNAc	956:971	the first GlcNAc in the Golgi	956:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	0	17	theme	Golgi	2:6	arg1	transporter					19:29	A Golgi UDP-GlcNAc transporter	0:29	A Golgi UDP-GlcNAc transporter	0:29	A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids.					
30224661	7	18	theme	loss-of-function	1193:1208	arg1	alleles					1210:1216	the loss-of-function alleles	1189:1216	the loss-of-function alleles affecting ROCK1	1189:1232	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
30224661	2	19	theme	cytosolic-derived	343:359	arg1	sugars					372:377	cytosolic-derived nucleotide sugars	343:377	cytosolic-derived nucleotide sugars	343:377	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	5	20	theme	GlcNAc	966:971	arg1	addition					944:951	the addition	940:951	the addition of the first GlcNAc in the Golgi	940:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	6	21	theme	sphingolipid	1002:1013	arg1	profiling					1015:1023	sphingolipid profiling	1002:1023	sphingolipid profiling	1002:1023	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	6	22	theme	phosphorylceramides	1095:1113	arg1	biosynthesis					1043:1054	the biosynthesis	1039:1054	the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs)	1039:1121	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	6	22	theme	phosphorylceramides	1095:1113	arg1	reliant					1131:1137	reliant	1131:1137	reliant	1131:1137	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	1	23	from	donors	133:138	arg1	form					147:150	the form	143:150	the form of nucleotide sugars	143:171	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	4	24	theme	loss-of-function	736:751	arg1	allele					753:758	the ugnt1 loss-of-function allele	726:758	the ugnt1 loss-of-function allele	726:758	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	3	25	theme	sugar	516:520	arg1	transporter					522:532	a Golgi-localized nucleotide sugar transporter	487:532	a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana	487:558	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	6	26	theme	inositol	1086:1093	arg1	GIPCs					1116:1120	GIPCs	1116:1120	GIPCs	1116:1120	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	6	26	theme	inositol	1086:1093	arg1	phosphorylceramides					1095:1113	GlcNAc-containing glycosyl inositol phosphorylceramides	1059:1113	GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs)	1059:1121	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	3	27	from	thaliana	551:558	arg1	transporter					522:532	a Golgi-localized nucleotide sugar transporter	487:532	a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana	487:558	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	1	28	theme	activated	114:122	arg1	donors					133:138	activated glycosyl donors	114:138	activated glycosyl donors in the form of nucleotide sugars	114:171	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	3	29	theme	nucleotide	505:514	arg1	transporter					522:532	a Golgi-localized nucleotide sugar transporter	487:532	a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana	487:558	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	0	30	theme	UDP-GlcNAc	8:17	arg1	transporter					19:29	A Golgi UDP-GlcNAc transporter	0:29	A Golgi UDP-GlcNAc transporter	0:29	A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids.					
30224661	6	31	theme	glycosyl	1077:1084	arg1	GIPCs					1116:1120	GIPCs	1116:1120	GIPCs	1116:1120	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	6	31	theme	glycosyl	1077:1084	arg1	phosphorylceramides					1095:1113	GlcNAc-containing glycosyl inositol phosphorylceramides	1059:1113	GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs)	1059:1121	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	1	32	theme	glycosyl	124:131	arg1	donors					133:138	activated glycosyl donors	114:138	activated glycosyl donors in the form of nucleotide sugars	114:171	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	1	33	theme	protein	219:225	arg1	modifications					227:239	post-translational protein modifications	200:239	post-translational protein modifications	200:239	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	6	34	theme	GlcNAc-containing	1059:1075	arg1	GIPCs					1116:1120	GIPCs	1116:1120	GIPCs	1116:1120	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	6	34	theme	GlcNAc-containing	1059:1075	arg1	phosphorylceramides					1095:1113	GlcNAc-containing glycosyl inositol phosphorylceramides	1059:1113	GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs)	1059:1121	Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter.					
30224661	4	35	contain	carrying	717:724	arg1	plants					710:715	plants	710:715	plants carrying the ugnt1 loss-of-function allele	710:758	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	4	35	contain	carrying	717:724	arg2	allele					753:758	the ugnt1 loss-of-function allele	726:758	the ugnt1 loss-of-function allele	726:758	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	8	36	theme	sphingolipids	1526:1538	arg1	maturation					1480:1489	the maturation	1476:1489	the maturation of N-glycans	1476:1502	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	8	36	theme	sphingolipids	1526:1538	arg1	class					1517:1521	the GIPC class	1508:1521	the GIPC class of sphingolipids	1508:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	7	37	contain	carrying	1180:1187	arg1	plants					1173:1178	plants	1173:1178	plants	1173:1178	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
30224661	7	37	contain	carrying	1180:1187	arg2	alleles					1210:1216	the loss-of-function alleles	1189:1216	the loss-of-function alleles affecting ROCK1	1189:1232	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
30224661	8	38	theme	single	1405:1410	arg1	transporter					1423:1433	a single UDP-GlcNAc transporter	1403:1433	a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids	1403:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	4	39	theme	ugnt1	730:734	arg1	allele					753:758	the ugnt1 loss-of-function allele	726:758	the ugnt1 loss-of-function allele	726:758	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	4	40	theme	N-glycopeptides	680:694	arg1	Profiles					668:675	Profiles	668:675	Profiles of N-glycopeptides	668:694	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	5	41	from	addition	944:951	arg1	Golgi					980:984	the Golgi	976:984	the Golgi	976:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	3	42	theme	Golgi-localized	489:503	arg1	transporter					522:532	a Golgi-localized nucleotide sugar transporter	487:532	a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana	487:558	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	0	43	theme	N-linked	55:62	arg1	glycans					64:70	N-linked glycans	55:70	N-linked glycans	55:70	A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids.					
30224661	3	44	theme	Arabidopsis	539:549	arg1	thaliana					551:558	Arabidopsis thaliana	539:558	Arabidopsis thaliana	539:558	We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1).					
30224661	2	45	link	cytosolic-derived	343:359	arg1	sugars					372:377	cytosolic-derived nucleotide sugars	343:377	cytosolic-derived nucleotide sugars	343:377	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	4	46	theme	complex	784:790	arg1	devoid					774:779	devoid	774:779	devoid	774:779	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	4	47	theme	N-glycans	803:811	arg1	devoid					774:779	devoid	774:779	devoid	774:779	Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans.					
30224661	5	48	from	GlcNAc	966:971	arg1	Golgi					980:984	the Golgi	976:984	the Golgi	976:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	2	49	theme	nucleotide	361:370	arg1	sugars					372:377	cytosolic-derived nucleotide sugars	343:377	cytosolic-derived nucleotide sugars	343:377	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	5	50	theme	N-glycopeptide	827:840	arg1	population					842:851	the N-glycopeptide population	823:851	the N-glycopeptide population from these alleles	823:870	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	1	51	theme	post-translational	200:217	arg1	modifications					227:239	post-translational protein modifications	200:239	post-translational protein modifications	200:239	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	2	52	theme	sugar	453:457	arg1	transporters					459:470	nucleotide sugar transporters	442:470	nucleotide sugar transporters	442:470	Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters.					
30224661	8	53	theme	essential	1452:1460	arg1	substrate					1462:1470	an essential substrate	1449:1470	an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids	1449:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	1	54	theme	polysaccharide	260:273	arg1	biosynthesis					275:286	polysaccharide biosynthesis	260:286	polysaccharide biosynthesis	260:286	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	5	55	theme	high-mannose	882:893	arg1	structures					895:904	high-mannose structures	882:904	high-mannose structures	882:904	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	5	56	theme	prior	931:935	arg1	structures					920:929	structures	920:929	structures prior to the addition of the first GlcNAc in the Golgi	920:984	Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi.					
30224661	1	57	theme	nucleotide	155:164	arg1	sugars					166:171	nucleotide sugars	155:171	nucleotide sugars	155:171	Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis.					
30224661	8	58	theme	UDP-GlcNAc	1412:1421	arg1	transporter					1423:1433	a single UDP-GlcNAc transporter	1403:1433	a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids	1403:1538	Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.					
30224661	7	59	theme	N-glycan	1336:1343	arg1	profiles					1353:1360	N-glycan or GIPC profiles	1336:1360	N-glycan or GIPC profiles	1336:1360	By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles.					
29555702	11	0	theme	antigenic	2214:2222	arg1	structures					2224:2233	surface-exposed antigenic structures	2198:2233	surface-exposed antigenic structures that are involved in the interaction between bacteria and host	2198:2296	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	2	1	from	genotype	415:422	arg1	isolates					469:476	meningococcal isolates	455:476	meningococcal isolates	455:476	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	10	2	theme	N.	1789:1790	arg1	meningitidis					1792:1803	N. meningitidis	1789:1803	N. meningitidis	1789:1803	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	3	theme	locus	969:973	arg1	polymorphisms					975:987	All major pgl locus polymorphisms	955:987	All major pgl locus polymorphisms identified in Neisseria meningitidis to date	955:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	4	theme	glycan	1240:1245	arg1	phenotype					1247:1255	glycan phenotype	1240:1255	glycan phenotype	1240:1255	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	13	5	theme	glycan	2688:2693	arg1	composition					2695:2705	glycan composition	2688:2705	glycan composition	2688:2705	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	10	6	theme	variability	1824:1834	arg1	changes					1836:1842	the phase variability changes	1814:1842	the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans	1814:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	1	7	theme	due	297:299	arg1	systems					289:295	the O-linked protein glycosylation (pgl) systems	248:295	the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	248:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	6	8	theme	major	959:963	arg1	polymorphisms					975:987	All major pgl locus polymorphisms	955:987	All major pgl locus polymorphisms identified in Neisseria meningitidis to date	955:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	10	9	from	diversity	1776:1784	arg1	meningitidis					1792:1803	N. meningitidis	1789:1803	N. meningitidis	1789:1803	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	4	10	theme	O-linked	704:711	arg1	glycosylation					721:733	O-linked protein glycosylation	704:733	The O-linked protein glycosylation genes	700:739	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	5	11	theme	protein	922:928	arg1	phenotype					944:952	the protein glycosylation phenotype	918:952	the protein glycosylation phenotype	918:952	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	4	12	theme	glycosylation	721:733	arg1	genes					735:739	The O-linked protein glycosylation genes	700:739	The O-linked protein glycosylation genes	700:739	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	1	13	link	O-linked	252:259	arg1	glycosylation					269:281	the O-linked protein glycosylation	248:281	the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	248:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	1	13	link	O-linked	252:259	arg1	pgl					284:286	pgl	284:286	pgl	284:286	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	1	14	theme	glycan	215:220	arg1	diversity					222:230	significant glycan diversity	203:230	significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	203:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	6	15	dep	genotype	1224:1231	arg1	the					1216:1218	the	1216:1218	the	1216:1218	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	0	16	theme	Meningococcal	127:139	arg1	Isolates					150:157	Paired Meningococcal Carriage Isolates	120:157	Paired Meningococcal Carriage Isolates	120:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	3	17	theme	meningococcal	604:616	arg1	carriers					618:625	50 asymptomatic meningococcal carriers	588:625	50 asymptomatic meningococcal carriers	588:625	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	7	18	gly	glycoprotein	1418:1429	arg1	glycoprotein					1418:1429	a given glycoprotein	1410:1429	a given glycoprotein	1410:1429	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	7	19	theme	given	1412:1416	arg1	glycoprotein					1418:1429	a given glycoprotein	1410:1429	a given glycoprotein	1410:1429	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	8	20	theme	phase-variable	1496:1509	arg1	expression					1511:1520	phase-variable expression	1496:1520	phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase	1496:1585	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	8	20	theme	phase-variable	1496:1509	arg1	mechanism					1441:1449	The main mechanism	1432:1449	The main mechanism responsible for the observed differences	1432:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	4	21	link	O-linked	704:711	arg1	glycosylation					721:733	O-linked protein glycosylation	704:733	The O-linked protein glycosylation genes	700:739	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	10	22	theme	common	2076:2081	arg1	commensal					2083:2091	a common commensal	2074:2091	a common commensal in the upper respiratory tract of healthy humans	2074:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	22	theme	common	2076:2081	arg1	meningitidis					2046:2057	Neisseria meningitidis	2036:2057	Neisseria meningitidis	2036:2057	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	23	theme	glycoform	1873:1881	arg1	repertoire					1883:1892	the expressed glycoform repertoire	1859:1892	the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis	1859:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	23	theme	glycoform	1873:1881	arg1	problem					1983:1989	a serious global health problem	1959:1989	a serious global health problem	1959:1989	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	24	theme	Neisseria	1003:1011	arg1	meningitidis					1013:1024	Neisseria meningitidis	1003:1024	Neisseria meningitidis to date	1003:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	4	25	theme	Genome	780:785	arg1	tool					798:801	the Genome Comparator tool	776:801	the Genome Comparator tool	776:801	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	9	26	from	phenotype	1679:1687	arg1	collection					1708:1717	a larger strain collection	1692:1717	a larger strain collection	1692:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	27	from	characterization	1624:1639	arg1	collection					1708:1717	a larger strain collection	1692:1717	a larger strain collection	1692:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	2	28	theme	asymptomatic	522:533	arg1	carriage					535:542	short-term asymptomatic carriage	511:542	short-term asymptomatic carriage	511:542	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	8	29	theme	involved	1529:1536	arg1	glycosyltransferases					1538:1557	the involved glycosyltransferases	1525:1557	the involved glycosyltransferases	1525:1557	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	7	30	theme	glycan	1378:1383	arg1	structures					1385:1394	glycan structures	1378:1394	glycan structures	1378:1394	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	10	31	theme	upper	2100:2104	arg1	tract					2118:2122	the upper respiratory tract	2096:2122	the upper respiratory tract of healthy humans	2096:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	0	32	theme	Glycosylation	66:78	arg1	System					80:85	the O-Linked Protein Glycosylation System	45:85	the O-Linked Protein Glycosylation System	45:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	6	33	attach	present	1039:1045	arg2	polymorphisms					975:987	All major pgl locus polymorphisms	955:987	All major pgl locus polymorphisms identified in Neisseria meningitidis to date	955:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	33	attach	present	1039:1045	arg1	collection					1062:1071	our isolate collection	1050:1071	our isolate collection	1050:1071	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	0	34	from	Diversity	107:115	arg1	Isolates					150:157	Paired Meningococcal Carriage Isolates	120:157	Paired Meningococcal Carriage Isolates	120:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	1	35	theme	genus	179:183	arg1	Neisseria					185:193	the genus Neisseria	175:193	the genus Neisseria	175:193	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	8	36	theme	O-acetyltransferase	1567:1585	arg1	expression					1511:1520	phase-variable expression	1496:1520	phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase	1496:1585	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	8	36	theme	O-acetyltransferase	1567:1585	arg1	mechanism					1441:1449	The main mechanism	1432:1449	The main mechanism responsible for the observed differences	1432:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	10	37	theme	healthy	2127:2133	arg1	humans					2135:2140	healthy humans	2127:2140	healthy humans	2127:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	0	38	theme	Glycan	100:105	arg1	Diversity					107:115	High Glycan Diversity	95:115	High Glycan Diversity in Paired Meningococcal Carriage Isolates	95:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	9	39	from	genotype	1652:1659	arg1	collection					1708:1717	a larger strain collection	1692:1717	a larger strain collection	1692:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	6	40	theme	isolate	1054:1060	arg1	collection					1062:1071	our isolate collection	1050:1071	our isolate collection	1050:1071	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	41	from	collection	1062:1071	arg1	present					1039:1045	present	1039:1045	present	1039:1045	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	10	42	theme	important	1746:1754	arg1	insight					1756:1762	important insight	1746:1762	important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans	1746:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	9	43	theme	first	1618:1622	arg1	this					1606:1609	this	1606:1609	this	1606:1609	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	43	theme	first	1618:1622	arg1	characterization					1624:1639	the first characterization	1614:1639	the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection	1614:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	2	44	theme	meningococcal	455:467	arg1	isolates					469:476	meningococcal isolates	455:476	meningococcal isolates	455:476	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	11	45	theme	homologous	2326:2335	arg1	recombination					2337:2349	homologous recombination	2326:2349	homologous recombination	2326:2349	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	0	46	theme	Genotypic	0:8	arg1	Characterization					25:40	Genotypic and Phenotypic Characterization	0:40	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System	0:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	10	47	from	commensal	2083:2091	arg1	tract					2118:2122	the upper respiratory tract	2096:2122	the upper respiratory tract of healthy humans	2096:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	13	48	theme	Protein	2533:2539	arg1	systems					2555:2561	Protein glycosylation systems	2533:2561	Protein glycosylation systems	2533:2561	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	7	49	theme	different	1356:1364	arg1	variants					1366:1373	different variants	1356:1373	different variants of glycan structures	1356:1394	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	6	50	from	present	1039:1045	arg1	collection					1062:1071	our isolate collection	1050:1071	our isolate collection	1050:1071	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	0	51	theme	Phenotypic	14:23	arg1	Characterization					25:40	Genotypic and Phenotypic Characterization	0:40	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System	0:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	10	52	theme	phase	1818:1822	arg1	changes					1836:1842	the phase variability changes	1814:1842	the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans	1814:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	9	53	theme	pgl	1648:1650	arg1	genotype					1652:1659	pgl genotype	1648:1659	pgl genotype	1648:1659	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	3	54	attach	derived	575:581	arg2	isolates					566:573	Paired meningococcal isolates	545:573	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart,	545:654	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	3	54	attach	derived	575:581	arg1	carriers					618:625	50 asymptomatic meningococcal carriers	588:625	50 asymptomatic meningococcal carriers	588:625	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	6	55	theme	pglH	1113:1116	arg1	presence					1092:1099	the variable presence	1079:1099	the variable presence of pglG and pglH	1079:1116	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	13	56	dep	mechanisms	2641:2650	arg1	the					2637:2639	the	2637:2639	the	2637:2639	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	10	57	theme	global	1969:1974	arg1	repertoire					1883:1892	the expressed glycoform repertoire	1859:1892	the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis	1859:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	57	theme	global	1969:1974	arg1	problem					1983:1989	a serious global health problem	1959:1989	a serious global health problem	1959:1989	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	2	58	theme	pgl	411:413	arg1	genotype					415:422	pgl genotype	411:422	pgl genotype	411:422	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	1	59	theme	polymorphic	324:334	arg1	genes					336:340	polymorphic genes	324:340	polymorphic genes	324:340	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	7	60	theme	microheterogeneity	1327:1344	arg1	degree					1310:1315	a high degree	1303:1315	a high degree	1303:1315	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	3	61	theme	whole-genome	675:686	arg1	sequencing					688:697	whole-genome sequencing	675:697	whole-genome sequencing	675:697	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	5	62	theme	glycan-specific	861:875	arg1	Abs					889:891	Abs	889:891	Abs	889:891	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	5	62	theme	glycan-specific	861:875	arg1	antibodies					877:886	glycan-specific antibodies	861:886	glycan-specific antibodies (Abs)	861:892	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	2	63	from	phenotype	442:450	arg1	isolates					469:476	meningococcal isolates	455:476	meningococcal isolates	455:476	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	9	64	theme	phenotype	1679:1687	arg1	this					1606:1609	this	1606:1609	this	1606:1609	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	64	theme	phenotype	1679:1687	arg1	characterization					1624:1639	the first characterization	1614:1639	the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection	1614:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	6	65	theme	significant	1179:1189	arg1	changes					1191:1197	significant changes	1179:1197	significant changes	1179:1197	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	10	66	theme	carriage.IMPORTANCE	1915:1933	arg1	meningitis					1945:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	67	dep	identified	1168:1177	arg1	present					1039:1045	present	1039:1045	present	1039:1045	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	10	68	theme	glycan	1769:1774	arg1	diversity					1776:1784	glycan diversity	1769:1784	glycan diversity in N. meningitidis	1769:1803	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	7	69	located	found	1401:1405	arg2	variants					1366:1373	different variants	1356:1373	different variants of glycan structures	1356:1394	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	7	69	located	found	1401:1405	arg1	degree					1310:1315	a high degree	1303:1315	a high degree	1303:1315	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	7	69	located	found	1401:1405	arg1	glycoprotein					1418:1429	a given glycoprotein	1410:1429	a given glycoprotein	1410:1429	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	5	70	with	Immunoblotting	841:854	arg1	Abs					889:891	Abs	889:891	Abs	889:891	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	5	70	with	Immunoblotting	841:854	arg1	antibodies					877:886	glycan-specific antibodies	861:886	glycan-specific antibodies (Abs)	861:892	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	7	71	theme	high	1305:1308	arg1	degree					1310:1315	a high degree	1303:1315	a high degree	1303:1315	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	6	72	theme	pglG	1104:1107	arg1	presence					1092:1099	the variable presence	1079:1099	the variable presence of pglG and pglH	1079:1116	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	8	73	theme	observed	1471:1478	arg1	differences					1480:1490	the observed differences	1467:1490	the observed differences	1467:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	3	74	theme	Paired	545:550	arg1	isolates					566:573	Paired meningococcal isolates	545:573	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart,	545:654	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	1	75	theme	glycosylation	269:281	arg1	systems					289:295	the O-linked protein glycosylation (pgl) systems	248:295	the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	248:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	9	76	theme	strain	1701:1706	arg1	collection					1708:1717	a larger strain collection	1692:1717	a larger strain collection	1692:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	10	77	theme	major	2007:2011	arg1	organisms					2023:2031	the major causative organisms	2003:2031	the major causative organisms	2003:2031	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	13	78	theme	evolutionary	2656:2667	arg1	forces					2669:2674	evolutionary forces	2656:2674	evolutionary forces	2656:2674	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	11	79	theme	structures	2224:2233	arg1	biosynthesis					2182:2193	biosynthesis	2182:2193	biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host	2182:2296	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	6	80	theme	pgl	965:967	arg1	polymorphisms					975:987	All major pgl locus polymorphisms	955:987	All major pgl locus polymorphisms identified in Neisseria meningitidis to date	955:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	11	81	theme	surface-exposed	2198:2212	arg1	structures					2224:2233	surface-exposed antigenic structures	2198:2233	surface-exposed antigenic structures that are involved in the interaction between bacteria and host	2198:2296	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	10	82	theme	Neisseria	2036:2044	arg1	commensal					2083:2091	a common commensal	2074:2091	a common commensal in the upper respiratory tract of healthy humans	2074:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	82	theme	Neisseria	2036:2044	arg1	meningitidis					2046:2057	Neisseria meningitidis	2036:2057	Neisseria meningitidis	2036:2057	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	5	83	used	used	898:901	arg2	Immunoblotting					841:854	Immunoblotting	841:854	Immunoblotting with glycan-specific antibodies (Abs)	841:892	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	5	84	theme	glycosylation	930:942	arg1	phenotype					944:952	the protein glycosylation phenotype	918:952	the protein glycosylation phenotype	918:952	Immunoblotting with glycan-specific antibodies (Abs) was used to investigate the protein glycosylation phenotype.					
29555702	0	85	theme	O-Linked	49:56	arg1	Glycosylation					66:78	the O-Linked Protein Glycosylation	45:78	the O-Linked Protein Glycosylation System	45:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	6	86	theme	pgl	1220:1222	arg1	genotype					1224:1231	pgl genotype	1220:1231	pgl genotype	1220:1231	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	10	87	theme	organisms	2023:2031	arg1	organisms					2023:2031	the major causative organisms	2003:2031	the major causative organisms	2003:2031	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	87	theme	organisms	2023:2031	arg1	one					1996:1998	one	1996:1998	one	1996:1998	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	88	theme	expressed	1863:1871	arg1	repertoire					1883:1892	the expressed glycoform repertoire	1859:1892	the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis	1859:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	88	theme	expressed	1863:1871	arg1	problem					1983:1989	a serious global health problem	1959:1989	a serious global health problem	1959:1989	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	1	89	theme	significant	203:213	arg1	diversity					222:230	significant glycan diversity	203:230	significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	203:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	4	90	theme	protein	713:719	arg1	glycosylation					721:733	O-linked protein glycosylation	704:733	The O-linked protein glycosylation genes	700:739	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	0	91	theme	Carriage	141:148	arg1	Isolates					150:157	Paired Meningococcal Carriage Isolates	120:157	Paired Meningococcal Carriage Isolates	120:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	8	92	theme	main	1436:1439	arg1	expression					1511:1520	phase-variable expression	1496:1520	phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase	1496:1585	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	8	92	theme	main	1436:1439	arg1	mechanism					1441:1449	The main mechanism	1432:1449	The main mechanism responsible for the observed differences	1432:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	3	93	theme	asymptomatic	591:602	arg1	carriers					618:625	50 asymptomatic meningococcal carriers	588:625	50 asymptomatic meningococcal carriers	588:625	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
29555702	11	94	theme	numerous	2156:2163	arg1	loci					2165:2168	numerous loci	2156:2168	numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host	2156:2296	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	10	95	theme	meningococcal	1901:1913	arg1	meningitis					1945:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	96	theme	paired	1271:1276	arg1	isolates					1278:1285	the paired isolates	1267:1285	the paired isolates	1267:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	9	97	from	collection	1708:1717	arg1	this					1606:1609	this	1606:1609	this	1606:1609	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	97	from	collection	1708:1717	arg1	characterization					1624:1639	the first characterization	1614:1639	the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection	1614:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	12	98	theme	phase	2426:2430	arg1	variation					2432:2440	phase variation	2426:2440	phase variation	2426:2440	These mechanisms are well described in Neisseria, and phase variation provides the ability to change these structures reversibly in response to the environment.					
29555702	8	99	theme	glycosyltransferases	1538:1557	arg1	expression					1511:1520	phase-variable expression	1496:1520	phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase	1496:1585	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	8	99	theme	glycosyltransferases	1538:1557	arg1	mechanism					1441:1449	The main mechanism	1432:1449	The main mechanism responsible for the observed differences	1432:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	13	100	dep	becoming	2567:2574	arg1	identified					2583:2592	identified	2583:2592	are becoming widely identified in bacteria	2563:2604	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	10	101	theme	respiratory	2106:2116	arg1	tract					2118:2122	the upper respiratory tract	2096:2122	the upper respiratory tract of healthy humans	2096:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	0	102	theme	System	80:85	arg1	Characterization					25:40	Genotypic and Phenotypic Characterization	0:40	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System	0:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	10	103	theme	Bacterial	1935:1943	arg1	meningitis					1945:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	meningococcal carriage.IMPORTANCE Bacterial meningitis	1901:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	104	theme	variable	1083:1090	arg1	presence					1092:1099	the variable presence	1079:1099	the variable presence of pglG and pglH	1079:1116	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	2	105	theme	short-term	511:520	arg1	carriage					535:542	short-term asymptomatic carriage	511:542	short-term asymptomatic carriage	511:542	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	6	106	dep	collection	1062:1071	arg1	both					1119:1122	both	1119:1122	both	1119:1122	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	7	107	theme	structures	1385:1394	arg1	variants					1366:1373	different variants	1356:1373	different variants of glycan structures	1356:1394	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	1	108	theme	O-linked	252:259	arg1	glycosylation					269:281	the O-linked protein glycosylation	248:281	the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	248:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	1	108	theme	O-linked	252:259	arg1	pgl					284:286	pgl	284:286	pgl	284:286	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	0	109	theme	High	95:98	arg1	Diversity					107:115	High Glycan Diversity	95:115	High Glycan Diversity in Paired Meningococcal Carriage Isolates	95:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	10	110	theme	humans	2135:2140	arg1	tract					2118:2122	the upper respiratory tract	2096:2122	the upper respiratory tract of healthy humans	2096:2140	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	4	111	theme	https	810:814	arg1	database					831:838	the https://pubmlst.org/ database	806:838	the https://pubmlst.org/ database	806:838	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	0	112	theme	Paired	120:125	arg1	Isolates					150:157	Paired Meningococcal Carriage Isolates	120:157	Paired Meningococcal Carriage Isolates	120:157	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	11	113	theme	phase	2355:2359	arg1	variation					2361:2369	phase variation	2355:2369	phase variation	2355:2369	In bacteria, numerous loci involved in biosynthesis of surface-exposed antigenic structures that are involved in the interaction between bacteria and host are frequently subjected to homologous recombination and phase variation.					
29555702	2	114	from	changes	486:492	arg1	isolates					469:476	meningococcal isolates	455:476	meningococcal isolates	455:476	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	4	115	theme	//pubmlst.org/	816:829	arg1	database					831:838	the https://pubmlst.org/ database	806:838	the https://pubmlst.org/ database	806:838	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	4	116	theme	Comparator	787:796	arg1	tool					798:801	the Genome Comparator tool	776:801	the Genome Comparator tool	776:801	The O-linked protein glycosylation genes were characterized in detail using the Genome Comparator tool at the https://pubmlst.org/ database.					
29555702	6	117	from	diversity	1203:1211	arg1	genotype					1224:1231	pgl genotype	1220:1231	pgl genotype	1220:1231	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	117	from	diversity	1203:1211	arg1	phenotype					1247:1255	glycan phenotype	1240:1255	glycan phenotype	1240:1255	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	117	from	diversity	1203:1211	arg1	%					1262:1262	96%	1260:1262	96% of the paired isolates	1260:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	117	from	diversity	1203:1211	arg1	isolates					1278:1285	the paired isolates	1267:1285	the paired isolates	1267:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	1	118	theme	phase	304:308	arg1	variation					310:318	phase variation	304:318	phase variation	304:318	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	13	119	theme	glycosylation	2541:2553	arg1	systems					2555:2561	Protein glycosylation systems	2533:2561	Protein glycosylation systems	2533:2561	Protein glycosylation systems are becoming widely identified in bacteria, and yet little is known about the mechanisms and evolutionary forces influencing glycan composition during carriage and disease.					
29555702	6	120	theme	isolates	1278:1285	arg1	%					1262:1262	96%	1260:1262	96% of the paired isolates	1260:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	120	theme	isolates	1278:1285	arg1	isolates					1278:1285	the paired isolates	1267:1285	the paired isolates	1267:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	2	121	theme	glycosylation	428:440	arg1	phenotype					442:450	glycosylation phenotype	428:450	glycosylation phenotype	428:450	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	10	122	theme	health	1976:1981	arg1	repertoire					1883:1892	the expressed glycoform repertoire	1859:1892	the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis	1859:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	122	theme	health	1976:1981	arg1	problem					1983:1989	a serious global health problem	1959:1989	a serious global health problem	1959:1989	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	9	123	dep	genotype	1652:1659	arg1	the					1644:1646	the	1644:1646	the	1644:1646	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	124	theme	genotype	1652:1659	arg1	this					1606:1609	this	1606:1609	this	1606:1609	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	9	124	theme	genotype	1652:1659	arg1	characterization					1624:1639	the first characterization	1614:1639	the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection	1614:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	7	125	theme	glycan	1320:1325	arg1	microheterogeneity					1327:1344	glycan microheterogeneity	1320:1344	glycan microheterogeneity	1320:1344	There was also a high degree of glycan microheterogeneity, in which different variants of glycan structures were found at a given glycoprotein.					
29555702	10	126	theme	serious	1961:1967	arg1	repertoire					1883:1892	the expressed glycoform repertoire	1859:1892	the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis	1859:1954	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	10	126	theme	serious	1961:1967	arg1	problem					1983:1989	a serious global health problem	1959:1989	a serious global health problem	1959:1989	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	0	127	theme	Protein	58:64	arg1	Glycosylation					66:78	the O-Linked Protein Glycosylation	45:78	the O-Linked Protein Glycosylation System	45:85	Genotypic and Phenotypic Characterization of the O-Linked Protein Glycosylation System Reveals High Glycan Diversity in Paired Meningococcal Carriage Isolates.					
29555702	6	128	with	combination	1127:1137	arg1	pglB2					1159:1163	pglB2	1159:1163	pglB2	1159:1163	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	128	with	combination	1127:1137	arg1	pglB					1151:1154	pglB	1151:1154	pglB	1151:1154	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	9	129	theme	glycosylation	1665:1677	arg1	phenotype					1679:1687	glycosylation phenotype	1665:1687	glycosylation phenotype	1665:1687	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	2	130	dep	genotype	415:422	arg1	the					407:409	the	407:409	the	407:409	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	6	131	from	changes	1191:1197	arg1	genotype					1224:1231	pgl genotype	1220:1231	pgl genotype	1220:1231	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	131	from	changes	1191:1197	arg1	phenotype					1247:1255	glycan phenotype	1240:1255	glycan phenotype	1240:1255	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	131	from	changes	1191:1197	arg1	%					1262:1262	96%	1260:1262	96% of the paired isolates	1260:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	131	from	changes	1191:1197	arg1	isolates					1278:1285	the paired isolates	1267:1285	the paired isolates	1267:1285	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	1	132	theme	gene	346:349	arg1	content					351:357	gene content	346:357	gene content	346:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	8	133	theme	responsible	1451:1461	arg1	expression					1511:1520	phase-variable expression	1496:1520	phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase	1496:1585	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	8	133	theme	responsible	1451:1461	arg1	mechanism					1441:1449	The main mechanism	1432:1449	The main mechanism responsible for the observed differences	1432:1490	The main mechanism responsible for the observed differences was phase-variable expression of the involved glycosyltransferases and the O-acetyltransferase.					
29555702	2	134	theme	study	376:380	arg1	aim					364:366	The aim	360:366	The aim of this study	360:380	The aim of this study was to examine in detail the pgl genotype and glycosylation phenotype in meningococcal isolates and the changes occurring during short-term asymptomatic carriage.					
29555702	1	135	theme	protein	261:267	arg1	glycosylation					269:281	the O-linked protein glycosylation	248:281	the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content	248:357	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	1	135	theme	protein	261:267	arg1	pgl					284:286	pgl	284:286	pgl	284:286	Species within the genus Neisseria display significant glycan diversity associated with the O-linked protein glycosylation (pgl) systems due to phase variation and polymorphic genes and gene content.					
29555702	9	136	theme	larger	1694:1699	arg1	collection					1708:1717	a larger strain collection	1692:1717	a larger strain collection	1692:1717	To our knowledge, this is the first characterization of the pgl genotype and glycosylation phenotype in a larger strain collection.					
29555702	10	137	theme	causative	2013:2021	arg1	organisms					2023:2031	the major causative organisms	2003:2031	the major causative organisms	2003:2031	This report thus provides important insight into glycan diversity in N. meningitidis and into the phase variability changes that influence the expressed glycoform repertoire during meningococcal carriage.IMPORTANCE Bacterial meningitis is a serious global health problem, and one of the major causative organisms is Neisseria meningitidis, which is also a common commensal in the upper respiratory tract of healthy humans.					
29555702	6	138	located	present	1039:1045	arg2	polymorphisms					975:987	All major pgl locus polymorphisms	955:987	All major pgl locus polymorphisms identified in Neisseria meningitidis to date	955:1032	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	6	138	located	present	1039:1045	arg1	collection					1062:1071	our isolate collection	1050:1071	our isolate collection	1050:1071	All major pgl locus polymorphisms identified in Neisseria meningitidis to date were present in our isolate collection, with the variable presence of pglG and pglH, both in combination with either pglB or pglB2 We identified significant changes and diversity in the pgl genotype and/or glycan phenotype in 96% of the paired isolates.					
29555702	3	139	theme	meningococcal	552:564	arg1	isolates					566:573	Paired meningococcal isolates	545:573	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart,	545:654	Paired meningococcal isolates derived from 50 asymptomatic meningococcal carriers, taken about 2 months apart, were analyzed with whole-genome sequencing.					
30564965	4	0	theme	complex	692:698	arg1	N-glycans					705:713	multiantennary complex type N-glycans	677:713	multiantennary complex type N-glycans	677:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	5	1	theme	α2M	747:749	arg1	glycosylation					730:742	The increased glycosylation	716:742	The increased glycosylation of α2M	716:749	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	2	theme	reduced	770:776	arg1	binding					778:784	reduced binding	770:784	reduced binding of Zn ions	770:795	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	2	3	theme	biological	333:342	arg1	ageing					344:349	biological ageing	333:349	biological ageing	333:349	The degree of inflammation was recently proposed to be considered as a biomarker of biological ageing.					
30564965	5	4	theme	increased	720:728	arg1	glycosylation					730:742	The increased glycosylation	716:742	The increased glycosylation of α2M	716:749	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	6	5	with	patients	1084:1091	arg1	cancer					1104:1109	colon cancer	1098:1109	colon cancer	1098:1109	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	6	theme	colon	1098:1102	arg1	cancer					1104:1109	colon cancer	1098:1109	colon cancer	1098:1109	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	4	7	theme	increased	575:583	arg1	content					585:591	an increased content	572:591	an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans	572:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	6	8	theme	reactivity	886:895	arg1	Glycosylation					857:869	Glycosylation	857:869	Glycosylation of α2M and its reactivity with IGFBP-2	857:908	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	4	9	located	detected	531:538	arg1	individuals					549:559	older individuals	543:559	older individuals	543:559	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	4	9	located	detected	531:538	arg2	reactivity					488:497	Higher reactivity	481:497	Higher reactivity of α2M with several lectins	481:525	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	2	10	theme	ageing	344:349	arg1	biomarker					320:328	a biomarker	318:328	a biomarker of biological ageing	318:349	The degree of inflammation was recently proposed to be considered as a biomarker of biological ageing.					
30564965	2	10	theme	ageing	344:349	arg1	degree					253:258	The degree	249:258	The degree of inflammation	249:274	The degree of inflammation was recently proposed to be considered as a biomarker of biological ageing.					
30564965	7	11	theme	structural	1215:1224	arg1	changes					1226:1232	structural changes	1215:1232	structural changes in the glycan moieties of α2M	1215:1262	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	3	12	theme	protein	461:467	arg1	microarray					469:478	lectin-based protein microarray	448:478	lectin-based protein microarray	448:478	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	6	13	theme	Zn	1055:1056	arg1	ions					1058:1061	Zn ions	1055:1061	Zn ions	1055:1061	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	3	14	theme	ages	440:443	arg1	population					416:425	a human population	408:425	a human population of different ages	408:443	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	2	15	theme	inflammation	263:274	arg1	biomarker					320:328	a biomarker	318:328	a biomarker of biological ageing	318:349	The degree of inflammation was recently proposed to be considered as a biomarker of biological ageing.					
30564965	2	15	theme	inflammation	263:274	arg1	degree					253:258	The degree	249:258	The degree of inflammation	249:274	The degree of inflammation was recently proposed to be considered as a biomarker of biological ageing.					
30564965	4	16	theme	mannose	640:646	arg1	content					585:591	an increased content	572:591	an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans	572:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	5	17	theme	ions	792:795	arg1	binding					778:784	reduced binding	770:784	reduced binding of Zn ions	770:795	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	17	theme	ions	792:795	arg1	IGFBP-2					847:853	IGFBP-2	847:853	IGFBP-2	847:853	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	17	theme	ions	792:795	arg1	protein					836:842	insulin-like growth factor-binding protein 2	801:844	insulin-like growth factor-binding protein 2 (IGFBP-2)	801:854	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	6	18	with	α2M	874:876	arg1	IGFBP-2					902:908	IGFBP-2	902:908	IGFBP-2	902:908	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	19	theme	healthy	1123:1129	arg1	individuals					1143:1153	healthy middle-aged individuals	1123:1153	healthy middle-aged individuals	1123:1153	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	3	20	theme	human	410:414	arg1	population					416:425	a human population	408:425	a human population of different ages	408:443	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	6	21	theme	α2M	874:876	arg1	Glycosylation					857:869	Glycosylation	857:869	Glycosylation of α2M and its reactivity with IGFBP-2	857:908	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	7	22	theme	IGFBP-2	1177:1183	arg1	binding					1166:1172	the binding	1162:1172	the binding of IGFBP-2 to α2M	1162:1190	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	7	22	theme	IGFBP-2	1177:1183	arg1	related					1204:1210	related	1204:1210	related	1204:1210	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	5	23	gly	glycosylation	730:742	arg1	α2M					747:749	α2M	747:749	α2M	747:749	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	7	24	theme	α2M	1260:1262	arg1	moieties					1248:1255	the glycan moieties	1237:1255	the glycan moieties of α2M	1237:1262	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	5	25	theme	insulin-like	801:812	arg1	protein					836:842	insulin-like growth factor-binding protein 2	801:844	insulin-like growth factor-binding protein 2 (IGFBP-2)	801:854	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	25	theme	insulin-like	801:812	arg1	IGFBP-2					847:853	IGFBP-2	847:853	IGFBP-2	847:853	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	6	26	theme	cancer	965:970	arg1	ageing					935:940	ageing	935:940	ageing	935:940	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	26	theme	cancer	965:970	arg1	incidence					946:954	incidence	946:954	incidence of colon cancer	946:970	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	7	27	from	changes	1226:1232	arg1	moieties					1248:1255	the glycan moieties	1237:1255	the glycan moieties of α2M	1237:1262	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	6	28	gly	Glycosylation	857:869	arg1	α2M					874:876	α2M	874:876	α2M	874:876	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	28	gly	Glycosylation	857:869	arg1	reactivity					886:895	its reactivity	882:895	its reactivity with IGFBP-2	882:908	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	5	29	theme	growth	814:819	arg1	protein					836:842	insulin-like growth factor-binding protein 2	801:844	insulin-like growth factor-binding protein 2 (IGFBP-2)	801:854	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	29	theme	growth	814:819	arg1	IGFBP-2					847:853	IGFBP-2	847:853	IGFBP-2	847:853	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	6	30	theme	colon	959:963	arg1	cancer					965:970	colon cancer	959:970	colon cancer	959:970	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	0	31	with	Reactivity	79:88	arg1	Ligands					95:101	Ligands	95:101	Ligands	95:101	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	4	32	theme	α2,6	622:625	arg1	acid					634:637	α2,6 sialic acid	622:637	α2,6 sialic acid	622:637	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	5	33	theme	factor-binding	821:834	arg1	protein					836:842	insulin-like growth factor-binding protein 2	801:844	insulin-like growth factor-binding protein 2 (IGFBP-2)	801:854	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	5	33	theme	factor-binding	821:834	arg1	IGFBP-2					847:853	IGFBP-2	847:853	IGFBP-2	847:853	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	4	34	theme	older	543:547	arg1	individuals					549:559	older individuals	543:559	older individuals	543:559	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	6	35	theme	middle-aged	1131:1141	arg1	individuals					1143:1153	healthy middle-aged individuals	1123:1153	healthy middle-aged individuals	1123:1153	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	4	36	theme	multiantennary	677:690	arg1	N-glycans					705:713	multiantennary complex type N-glycans	677:713	multiantennary complex type N-glycans	677:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	1	37	theme	chronic	190:196	arg1	inflammation					198:209	chronic inflammation	190:209	chronic inflammation	190:209	Alpha-2-macroglobulin (α2M) is a molecule generally associated with inflammation, and chronic inflammation is associated with ageing and cancer.					
30564965	7	38	theme	glycan	1241:1246	arg1	moieties					1248:1255	the glycan moieties	1237:1255	the glycan moieties of α2M	1237:1262	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	4	39	with	reactivity	488:497	arg1	lectins					519:525	several lectins	511:525	several lectins	511:525	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	4	40	theme	acid	634:637	arg1	content					585:591	an increased content	572:591	an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans	572:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	6	41	theme	α2M	995:997	arg1	reactivity					981:990	the reactivity	977:990	the reactivity of α2M with Zn ions	977:1010	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	0	42	with	Ageing	15:20	arg1	Ligands					95:101	Ligands	95:101	Ligands	95:101	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	4	43	theme	sialic	627:632	arg1	acid					634:637	α2,6 sialic acid	622:637	α2,6 sialic acid	622:637	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	5	44	theme	Zn	789:790	arg1	ions					792:795	Zn ions	789:795	Zn ions	789:795	The increased glycosylation of α2M was accompanied by reduced binding of Zn ions and insulin-like growth factor-binding protein 2 (IGFBP-2).					
30564965	4	45	theme	α2M	502:504	arg1	reactivity					488:497	Higher reactivity	481:497	Higher reactivity of α2M with several lectins	481:525	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	3	46	attach	attached	375:382	arg1	α2M					387:389	α2M	387:389	α2M	387:389	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	3	46	attach	attached	375:382	arg2	glycans					367:373	glycans	367:373	glycans attached to α2M	367:389	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	6	47	from	unaltered	1071:1079	arg1	patients					1084:1091	patients	1084:1091	patients with colon cancer	1084:1109	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	4	48	theme	N-glycans	705:713	arg1	content					585:591	an increased content	572:591	an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans	572:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	0	49	theme	Glycan	29:34	arg1	Structure					36:44	the Glycan Structure	25:44	the Glycan Structure of Alpha-2-Macroglobulin	25:69	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	4	50	theme	Higher	481:486	arg1	reactivity					488:497	Higher reactivity	481:497	Higher reactivity of α2M with several lectins	481:525	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	4	51	theme	type	700:703	arg1	N-glycans					705:713	multiantennary complex type N-glycans	677:713	multiantennary complex type N-glycans	677:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	4	52	theme	several	511:517	arg1	lectins					519:525	several lectins	511:525	several lectins	511:525	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	7	53	theme	ions	1287:1290	arg1	binding					1273:1279	binding	1273:1279	binding of Zn ions	1273:1290	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	6	54	from	patients	1084:1091	arg1	unaltered					1071:1079	unaltered	1071:1079	unaltered	1071:1079	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	55	theme	Zn	1004:1005	arg1	ions					1007:1010	Zn ions	1004:1010	Zn ions	1004:1010	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	6	56	with	reactivity	886:895	arg1	IGFBP-2					902:908	IGFBP-2	902:908	IGFBP-2	902:908	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	3	57	theme	lectin-based	448:459	arg1	microarray					469:478	lectin-based protein microarray	448:478	lectin-based protein microarray	448:478	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	0	58	theme	Alpha-2-Macroglobulin	49:69	arg1	Structure					36:44	the Glycan Structure	25:44	the Glycan Structure of Alpha-2-Macroglobulin	25:69	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	0	59	from	Reactivity	79:88	arg1	Structure					36:44	the Glycan Structure	25:44	the Glycan Structure of Alpha-2-Macroglobulin	25:69	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	6	60	with	reactivity	981:990	arg1	ions					1007:1010	Zn ions	1004:1010	Zn ions	1004:1010	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	3	61	theme	different	430:438	arg1	ages					440:443	different ages	430:443	different ages	430:443	In this study, glycans attached to α2M were analysed in a human population of different ages by lectin-based protein microarray.					
30564965	4	62	theme	monosaccharides	605:619	arg1	content					585:591	an increased content	572:591	an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans	572:713	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30564965	0	63	from	Ageing	15:20	arg1	Structure					36:44	the Glycan Structure	25:44	the Glycan Structure of Alpha-2-Macroglobulin	25:69	Changes Due to Ageing in the Glycan Structure of Alpha-2-Macroglobulin and Its Reactivity with Ligands.					
30564965	7	64	theme	Zn	1284:1285	arg1	ions					1287:1290	Zn ions	1284:1290	Zn ions	1284:1290	Thus, the binding of IGFBP-2 to α2M seems to be related to structural changes in the glycan moieties of α2M, whereas binding of Zn ions, most likely, is not.					
30564965	6	65	theme	ions	1058:1061	arg1	binding					1044:1050	the binding	1040:1050	the binding of Zn ions	1040:1061	Glycosylation of α2M and its reactivity with IGFBP-2 is similarly affected by ageing and incidence of colon cancer, but the reactivity of α2M with Zn ions is differently affected, as the binding of Zn ions remains unaltered in patients with colon cancer compared to healthy middle-aged individuals.					
30564965	4	66	theme	specific	596:603	arg1	monosaccharides					605:619	specific monosaccharides	596:619	specific monosaccharides	596:619	Higher reactivity of α2M with several lectins was detected in older individuals indicating an increased content of specific monosaccharides: α2,6 sialic acid, mannose and N-acetylglucosamine, and multiantennary complex type N-glycans.					
30466347	5	0	with	EndoS2	1053:1058	arg1	activity					1099:1106	transglycosylation activity	1080:1106	transglycosylation activity	1080:1106	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	1	from	effect	1243:1248	arg1	functions					1292:1300	Fc effector functions	1280:1300	Fc effector functions	1280:1300	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	1	from	effect	1243:1248	arg1	stability					1314:1322	protein stability	1306:1322	protein stability	1306:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	2	2	theme	attributes	363:372	arg1	one					276:278	one	276:278	one	276:278	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	2	theme	attributes	363:372	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	2	theme	attributes	363:372	arg1	modifications					318:330	most common post-translational modifications	287:330	most common post-translational modifications	287:330	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	5	3	theme	Fc	1280:1281	arg1	functions					1292:1300	Fc effector functions	1280:1300	Fc effector functions	1280:1300	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	6	4	dep	fucosylation	1355:1366	arg1	only					1472:1475	only	1472:1475	only	1472:1475	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	6	5	theme	core	1350:1353	arg1	fucosylation					1355:1366	the core fucosylation	1346:1366	the core fucosylation	1346:1366	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	4	6	theme	biological	677:686	arg1	relevance					703:711	the biological and functional relevance	673:711	the biological and functional relevance of glycosylation	673:728	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	2	7	theme	post-translational	299:316	arg1	modifications					318:330	most common post-translational modifications	287:330	most common post-translational modifications	287:330	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	6	8	theme	protein	1622:1628	arg1	aggregation					1630:1640	protein aggregation	1622:1640	protein aggregation	1622:1640	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	5	9	used	used	1112:1115	arg2	glycoengineering					979:994	chemoenzymatic glycoengineering	964:994	chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity	964:1106	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	10	theme	mAb	1129:1131	arg1	glycoforms					1133:1142	mAb glycoforms	1129:1142	mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability	1129:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	1	11	theme	IgG	219:221	arg1	chain					210:214	each heavy chain	199:214	each heavy chain of IgG in the CH2 domain	199:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	4	12	theme	glycoforms	647:656	arg1	separation					625:634	separation	625:634	separation of various glycoforms to investigate the biological and functional relevance of glycosylation	625:728	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	4	12	theme	glycoforms	647:656	arg1	challenge					741:749	a major challenge	733:749	a major challenge	733:749	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	1	13	gly	Glycosylation	146:158	arg1	residue					188:194	the conserved asparagine residue	163:194	the conserved asparagine residue in each heavy chain of IgG in the CH2 domain	163:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	4	14	theme	major	735:739	arg1	challenge					741:749	a major challenge	733:749	a major challenge	733:749	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	4	14	theme	major	735:739	arg1	separation					625:634	separation	625:634	separation of various glycoforms to investigate the biological and functional relevance of glycosylation	625:728	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	1	15	from	domain	234:239	arg1	chain					210:214	each heavy chain	199:214	each heavy chain of IgG in the CH2 domain	199:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	2	16	dep	modifications	318:330	arg1	the					283:285	the	283:285	the	283:285	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	4	17	theme	functional	692:701	arg1	relevance					703:711	the biological and functional relevance	673:711	the biological and functional relevance of glycosylation	673:728	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	6	18	theme	FcγRIIIa	1480:1487	arg1	binding					1489:1495	FcγRIIIa binding	1480:1495	FcγRIIIa binding	1480:1495	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	1	19	theme	conserved	167:175	arg1	residue					188:194	the conserved asparagine residue	163:194	the conserved asparagine residue in each heavy chain of IgG in the CH2 domain	163:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	6	20	theme	terminal	1382:1389	arg1	galactosylation					1391:1405	non-reducing terminal galactosylation	1369:1405	non-reducing terminal galactosylation	1369:1405	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	0	21	theme	glycoengineered	76:90	arg1	antibody					108:115	glycoengineered IgG1 monoclonal antibody	76:115	glycoengineered IgG1 monoclonal antibody	76:115	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	1	22	theme	residue	188:194	arg1	Glycosylation					146:158	Glycosylation	146:158	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain	146:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	1	22	theme	residue	188:194	arg1	N-glycosylation					253:267	N-glycosylation	253:267	N-glycosylation	253:267	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	0	23	from	Influence	0:8	arg1	functions					41:49	effector functions	32:49	effector functions	32:49	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	0	23	from	Influence	0:8	arg1	stability					63:71	thermal stability	55:71	thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms	55:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	0	24	theme	monoclonal	97:106	arg1	antibody					108:115	glycoengineered IgG1 monoclonal antibody	76:115	glycoengineered IgG1 monoclonal antibody	76:115	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	5	25	theme	homogeneous	1156:1166	arg1	N-glycans					1185:1193	highly homogeneous and well-defined N-glycans	1149:1193	highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability	1149:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	26	theme	endo-β-N-acetylglucosaminidase	1013:1042	arg1	EndoS2					1053:1058	an endo-β-N-acetylglucosaminidase (ENGase) EndoS2	1010:1058	an endo-β-N-acetylglucosaminidase (ENGase) EndoS2	1010:1058	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	26	theme	endo-β-N-acetylglucosaminidase	1013:1042	arg1	ENGase					1045:1050	ENGase	1045:1050	ENGase	1045:1050	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	2	27	theme	therapeutics	403:414	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	27	theme	therapeutics	403:414	arg1	modifications					318:330	most common post-translational modifications	287:330	most common post-translational modifications	287:330	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	6	28	theme	IgG1 mAb	1442:1449	arg1	impact					1461:1466	IgG1 mAb N-glycans impact	1442:1466	IgG1 mAb N-glycans impact	1442:1466	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	5	29	theme	well-defined	1172:1183	arg1	N-glycans					1185:1193	highly homogeneous and well-defined N-glycans	1149:1193	highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability	1149:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	1	30	from	Glycosylation	146:158	arg1	chain					210:214	each heavy chain	199:214	each heavy chain of IgG in the CH2 domain	199:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	6	31	theme	C1q	1549:1551	arg1	binding					1553:1559	C1q binding	1549:1559	C1q binding	1549:1559	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	5	32	theme	chemoenzymatic	964:977	arg1	glycoengineering					979:994	chemoenzymatic glycoengineering	964:994	chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity	964:1106	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	4	33	theme	existing	756:763	arg1	studies					765:771	existing studies	756:771	existing studies	756:771	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	3	34	theme	N-glycosylation	473:487	arg1	effects					455:461	the effects	451:461	the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway	451:613	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	0	35	theme	N-glycosylation	13:27	arg1	Influence					0:8	Influence	0:8	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.	0:144	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	5	36	theme	N-glycan	1258:1265	arg1	structure					1267:1275	each N-glycan structure	1253:1275	each N-glycan structure	1253:1275	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	5	37	with	glycoforms	1133:1142	arg1	N-glycans					1185:1193	highly homogeneous and well-defined N-glycans	1149:1193	highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability	1149:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	0	38	theme	effector	32:39	arg1	functions					41:49	effector functions	32:49	effector functions	32:49	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	6	39	theme	cell-mediated	1517:1529	arg1	cytotoxicity					1531:1542	antibody-dependent cell-mediated cytotoxicity	1498:1542	antibody-dependent cell-mediated cytotoxicity	1498:1542	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	3	40	theme	Fc	500:501	arg1	functions					512:520	Fc effector functions	500:520	Fc effector functions	500:520	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	5	41	gly	glycoforms	1133:1142	arg1	mAb					1129:1131	mAb glycoforms	1129:1142	mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability	1129:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	2	42	theme	important	336:344	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	6	43	theme	thermal	1585:1591	arg1	stability					1593:1601	thermal stability	1585:1601	thermal stability	1585:1601	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	1	44	theme	CH2	230:232	arg1	domain					234:239	the CH2 domain	226:239	the CH2 domain	226:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	4	45	theme	N-glycans	809:817	arg1	impact					799:804	the overall impact	787:804	the overall impact of N-glycans	787:817	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	2	46	theme	antibody	388:395	arg1	therapeutics					403:414	monoclonal antibody (mAb) therapeutics	377:414	monoclonal antibody (mAb) therapeutics	377:414	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	6	47	theme	antibody-dependent	1498:1515	arg1	cytotoxicity					1531:1542	antibody-dependent cell-mediated cytotoxicity	1498:1542	antibody-dependent cell-mediated cytotoxicity	1498:1542	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	6	48	theme	non-reducing	1369:1380	arg1	galactosylation					1391:1405	non-reducing terminal galactosylation	1369:1405	non-reducing terminal galactosylation	1369:1405	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	4	49	theme	glycoform	877:885	arg1	contributions					855:867	the individual contributions	840:867	the individual contributions of each glycoform	840:885	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	0	50	gly	N-glycosylation	13:27	arg1	antibody					108:115	glycoengineered IgG1 monoclonal antibody	76:115	glycoengineered IgG1 monoclonal antibody	76:115	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	0	50	gly	N-glycosylation	13:27	arg1	effector					32:39	effector functions	32:49	effector functions	32:49	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	3	51	theme	dependent	550:558	arg1	safety					492:497	safety	492:497	safety	492:497	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	5	52	theme	effector	1283:1290	arg1	functions					1292:1300	Fc effector functions	1280:1300	Fc effector functions	1280:1300	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	3	53	theme	neonatal	579:586	arg1	FcRn					601:604	FcRn	601:604	FcRn	601:604	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	53	theme	neonatal	579:586	arg1	receptor					591:598	neonatal Fc receptor	579:598	neonatal Fc receptor (FcRn) pathway	579:613	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	5	54	with	mutant	1068:1073	arg1	activity					1099:1106	transglycosylation activity	1080:1106	transglycosylation activity	1080:1106	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	2	55	theme	quality	355:361	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	0	56	with	stability	63:71	arg1	glycoforms					134:143	homogeneous glycoforms	122:143	homogeneous glycoforms	122:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	4	57	theme	individual	844:853	arg1	contributions					855:867	the individual contributions	840:867	the individual contributions of each glycoform	840:885	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	2	58	theme	modifications	318:330	arg1	one					276:278	one	276:278	one	276:278	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	58	theme	modifications	318:330	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	58	theme	modifications	318:330	arg1	modifications					318:330	most common post-translational modifications	287:330	most common post-translational modifications	287:330	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	1	59	theme	heavy	204:208	arg1	chain					210:214	each heavy chain	199:214	each heavy chain of IgG in the CH2 domain	199:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	2	60	theme	common	292:297	arg1	modifications					318:330	most common post-translational modifications	287:330	most common post-translational modifications	287:330	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	3	61	theme	Fc	588:589	arg1	FcRn					601:604	FcRn	601:604	FcRn	601:604	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	61	theme	Fc	588:589	arg1	receptor					591:598	neonatal Fc receptor	579:598	neonatal Fc receptor (FcRn) pathway	579:613	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	6	62	gly	fucosylation	1355:1366	arg1	impact					1461:1466	IgG1 mAb N-glycans impact	1442:1466	IgG1 mAb N-glycans impact	1442:1466	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	2	63	theme	critical	346:353	arg1	attributes					363:372	important critical quality attributes	336:372	important critical quality attributes	336:372	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	4	64	theme	various	639:645	arg1	glycoforms					647:656	various glycoforms	639:656	various glycoforms	639:656	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	3	65	theme	receptor	591:598	arg1	pathway					607:613	neonatal Fc receptor (FcRn) pathway	579:613	neonatal Fc receptor (FcRn) pathway	579:613	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	4	66	theme	glycosylation	716:728	arg1	relevance					703:711	the biological and functional relevance	673:711	the biological and functional relevance of glycosylation	673:728	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	1	67	from	IgG	219:221	arg1	domain					234:239	the CH2 domain	226:239	the CH2 domain	226:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	3	68	theme	independent	564:574	arg1	safety					492:497	safety	492:497	safety	492:497	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	4	69	theme	heterogeneous	920:932	arg1	distributions					934:946	highly heterogeneous distributions	913:946	highly heterogeneous distributions	913:946	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	3	70	theme	pathway	607:613	arg1	independent					564:574	independent	564:574	independent	564:574	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	1	71	from	chain	210:214	arg1	Glycosylation					146:158	Glycosylation	146:158	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain	146:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	1	71	from	chain	210:214	arg1	N-glycosylation					253:267	N-glycosylation	253:267	N-glycosylation	253:267	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	1	71	from	chain	210:214	arg1	domain					234:239	the CH2 domain	226:239	the CH2 domain	226:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	6	72	theme	impact	1461:1466	arg1	fucosylation					1355:1366	the core fucosylation	1346:1366	the core fucosylation	1346:1366	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	0	73	theme	IgG1	92:95	arg1	antibody					108:115	glycoengineered IgG1 monoclonal antibody	76:115	glycoengineered IgG1 monoclonal antibody	76:115	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	1	74	theme	asparagine	177:186	arg1	residue					188:194	the conserved asparagine residue	163:194	the conserved asparagine residue in each heavy chain of IgG in the CH2 domain	163:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	0	75	theme	antibody	108:115	arg1	functions					41:49	effector functions	32:49	effector functions	32:49	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	0	75	theme	antibody	108:115	arg1	stability					63:71	thermal stability	55:71	thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms	55:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	1	76	from	residue	188:194	arg1	chain					210:214	each heavy chain	199:214	each heavy chain of IgG in the CH2 domain	199:239	Glycosylation of the conserved asparagine residue in each heavy chain of IgG in the CH2 domain is known as N-glycosylation.					
30466347	6	77	theme	N-glycans	1451:1459	arg1	impact					1461:1466	IgG1 mAb N-glycans impact	1442:1466	IgG1 mAb N-glycans impact	1442:1466	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
30466347	0	78	theme	homogeneous	122:132	arg1	glycoforms					134:143	homogeneous glycoforms	122:143	homogeneous glycoforms	122:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	5	79	theme	structure	1267:1275	arg1	effect					1243:1248	the effect	1239:1248	the effect of each N-glycan structure on Fc effector functions and protein stability	1239:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	3	80	gly	N-glycosylation	473:487	arg1	Fc					500:501	Fc effector functions	500:520	Fc effector functions	500:520	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	80	gly	N-glycosylation	473:487	arg1	effector					503:510	Fc effector functions	500:520	Fc effector functions	500:520	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	81	theme	Fc	470:471	arg1	N-glycosylation					473:487	the Fc N-glycosylation	466:487	the Fc N-glycosylation	466:487	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	0	82	theme	thermal	55:61	arg1	stability					63:71	thermal stability	55:71	thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms	55:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	5	83	theme	transglycosylation	1080:1097	arg1	activity					1099:1106	transglycosylation activity	1080:1106	transglycosylation activity	1080:1106	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	3	84	theme	Various	417:423	arg1	studies					425:431	Various studies	417:431	Various studies	417:431	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	85	theme	effector	503:510	arg1	functions					512:520	Fc effector functions	500:520	Fc effector functions	500:520	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	0	86	with	functions	41:49	arg1	glycoforms					134:143	homogeneous glycoforms	122:143	homogeneous glycoforms	122:143	Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.					
30466347	4	87	theme	overall	791:797	arg1	impact					799:804	the overall impact	787:804	the overall impact of N-glycans	787:817	However, separation of various glycoforms to investigate the biological and functional relevance of glycosylation is a major challenge, and existing studies often discuss the overall impact of N-glycans, without considering the individual contributions of each glycoform when evaluating mAbs with highly heterogeneous distributions.					
30466347	5	88	theme	protein	1306:1312	arg1	stability					1314:1322	protein stability	1306:1322	protein stability	1306:1322	In this study, chemoenzymatic glycoengineering incorporating an endo-β-N-acetylglucosaminidase (ENGase) EndoS2 and its mutant with transglycosylation activity was used to generate mAb glycoforms with highly homogeneous and well-defined N-glycans to better understand and precisely evaluate the effect of each N-glycan structure on Fc effector functions and protein stability.					
30466347	3	89	from	effects	455:461	arg1	safety					492:497	safety	492:497	safety	492:497	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	89	from	effects	455:461	arg1	functions					512:520	Fc effector functions	500:520	Fc effector functions	500:520	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	3	89	from	effects	455:461	arg1	pharmacokinetics					527:542	pharmacokinetics	527:542	pharmacokinetics	527:542	Various studies have demonstrated the effects of the Fc N-glycosylation on safety, Fc effector functions, and pharmacokinetics, both dependent and independent of neonatal Fc receptor (FcRn) pathway.					
30466347	2	90	theme	monoclonal	377:386	arg1	mAb					398:400	mAb	398:400	mAb	398:400	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	2	90	theme	monoclonal	377:386	arg1	antibody					388:395	monoclonal antibody	377:395	monoclonal antibody (mAb) therapeutics	377:414	It is one of the most common post-translational modifications and important critical quality attributes of monoclonal antibody (mAb) therapeutics.					
30466347	6	91	theme	FcRn	1571:1574	arg1	binding					1576:1582	FcRn binding	1571:1582	FcRn binding	1571:1582	We demonstrated that the core fucosylation, non-reducing terminal galactosylation, sialylation, and mannosylation of IgG1 mAb N-glycans impact not only on FcγRIIIa binding, antibody-dependent cell-mediated cytotoxicity, and C1q binding, but also FcRn binding, thermal stability and propensity for protein aggregation.					
31329418	5	0	theme	method	1417:1422	arg1	applicability					1367:1379	the great applicability	1357:1379	the great applicability	1357:1379	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	0	theme	method	1417:1422	arg1	usage					1404:1408	potential clinical usage	1385:1408	potential clinical usage of the method	1385:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	1	theme	sialylated	1189:1198	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	4	2	theme	sialylglycan	949:960	arg1	compositions					962:973	28 sialylglycan compositions	946:973	28 sialylglycan compositions with Neu5Gc residues	946:994	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	3	theme	bovine	822:827	arg1	FBS					836:838	FBS	836:838	FBS	836:838	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	3	theme	bovine	822:827	arg1	serum					829:833	fetal bovine serum	816:833	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	5	4	theme	55	1274:1275	arg1	%					1276:1276	%	1276:1276	%	1276:1276	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	5	gly	α-2,6-sialylated	1290:1305	arg1	N-glycans					1319:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	6	gly	fucosylated	1307:1317	arg1	N-glycans					1319:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	7	theme	high-sensitivity	516:531	arg1	quantification					536:549	high-sensitivity MS quantification	516:549	high-sensitivity MS quantification	516:549	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	1	8	from	Changes	80:86	arg1	sialylglycans					133:145	sialylglycans	133:145	sialylglycans	133:145	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	5	9	theme	%	1276:1276	arg1	increase					1278:1285	a 55% increase	1272:1285	a 55% increase of α-2,6-sialylated fucosylated N-glycans	1272:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	1	10	link	α-2,3-/α-2,6-linked	91:109	arg1	SAs					125:127	SAs	125:127	SAs	125:127	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	1	10	link	α-2,3-/α-2,6-linked	91:109	arg1	acids					118:122	α-2,3-/α-2,6-linked sialic acids	91:122	α-2,3-/α-2,6-linked sialic acids (SAs)	91:128	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	5	11	theme	biomarker	1209:1217	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	4	12	theme	structural	768:777	arg1	identification					779:792	detailed structural identification	759:792	detailed structural identification	759:792	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	3	13	theme	sialylglycan	562:573	arg1	isomers					575:581	various sialylglycan isomers	554:581	various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	554:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	3	14	theme	isotopic	592:599	arg1	labeling					601:608	isotopic labeling	592:608	isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	592:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	3	15	theme	isomers	575:581	arg1	elution					504:510	sequential chromatographic elution	477:510	sequential chromatographic elution	477:510	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	3	15	theme	isomers	575:581	arg1	quantification					536:549	high-sensitivity MS quantification	516:549	high-sensitivity MS quantification	516:549	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	16	gly	sialylated	1189:1198	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	17	theme	chromatographic	488:502	arg1	elution					504:510	sequential chromatographic elution	477:510	sequential chromatographic elution	477:510	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	4	18	theme	many	885:888	arg1	isomers					900:906	many branching isomers	885:906	many branching isomers	885:906	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	1	19	theme	α-2,3-/α-2,6-linked	91:109	arg1	SAs					125:127	SAs	125:127	SAs	125:127	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	1	19	theme	α-2,3-/α-2,6-linked	91:109	arg1	acids					118:122	α-2,3-/α-2,6-linked sialic acids	91:122	α-2,3-/α-2,6-linked sialic acids (SAs)	91:128	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	4	20	theme	N-linked	841:848	arg1	isomers					863:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	1	21	theme	sialic	111:116	arg1	SAs					125:127	SAs	125:127	SAs	125:127	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	1	21	theme	sialic	111:116	arg1	acids					118:122	α-2,3-/α-2,6-linked sialic acids	91:122	α-2,3-/α-2,6-linked sialic acids (SAs)	91:128	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	5	22	gly	sialylated	1094:1103	arg1	N-glycans					1105:1113	sialylated N-glycans	1094:1113	sialylated N-glycans	1094:1113	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	23	theme	clinical	1395:1402	arg1	usage					1404:1408	potential clinical usage	1385:1408	potential clinical usage of the method	1385:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	1	24	theme	acids	118:122	arg1	Changes					80:86	Changes	80:86	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans	80:145	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	3	25	theme	analytical	422:431	arg1	strategy					433:440	an analytical strategy	419:440	an analytical strategy	419:440	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	26	theme	N-glycan	1200:1207	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	4	27	theme	branching	890:898	arg1	isomers					900:906	many branching isomers	885:906	many branching isomers	885:906	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	2	28	theme	structural	323:332	arg1	complexity					334:343	structural complexity	323:343	structural complexity	323:343	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	4	29	theme	fetal	816:820	arg1	FBS					836:838	FBS	836:838	FBS	836:838	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	29	theme	fetal	816:820	arg1	serum					829:833	fetal bovine serum	816:833	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	2	30	theme	detection	377:385	arg1	sensitivity					387:397	detection sensitivity	377:397	detection sensitivity	377:397	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	3	31	theme	online	635:640	arg1	chromatography					681:694	online reversed-phase high performance liquid chromatography	635:694	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	4	32	theme	Neu5Gc	980:985	arg1	residues					987:994	Neu5Gc residues	980:994	Neu5Gc residues	980:994	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	5	33	theme	bovine	1123:1128	arg1	IgG					1159:1161	IgG	1159:1161	IgG	1159:1161	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	33	theme	bovine	1123:1128	arg1	G					1156:1156	bovine and rabbit immunoglobulin G	1123:1156	bovine and rabbit immunoglobulin G (IgG)	1123:1162	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	4	34	link	N-linked	841:848	arg1	isomers					863:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	0	35	theme	Isomer-Specific	14:28	arg1	Quantification					30:43	Isomer-Specific Quantification	14:43	Isomer-Specific Quantification for Sialylation-Focused Glycomics	14:77	Glycoqueuing: Isomer-Specific Quantification for Sialylation-Focused Glycomics.					
31329418	4	36	theme	serum	829:833	arg1	isomers					863:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	37	theme	new	731:733	arg1	method					735:740	The new method	727:740	The new method	727:740	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	3	38	with	coupling	696:703	arg1	RP-HPLC-MS					714:723	RP-HPLC-MS	714:723	RP-HPLC-MS	714:723	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	3	38	with	coupling	696:703	arg1	MS					710:711	MS	710:711	MS (RP-HPLC-MS)	710:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	4	39	with	compositions	962:973	arg1	residues					987:994	Neu5Gc residues	980:994	Neu5Gc residues	980:994	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	0	40	theme	Sialylation-Focused	49:67	arg1	Glycomics					69:77	Sialylation-Focused Glycomics	49:77	Sialylation-Focused Glycomics	49:77	Glycoqueuing: Isomer-Specific Quantification for Sialylation-Focused Glycomics.					
31329418	2	41	dep	instability	310:320	arg1	sensitivity					387:397	detection sensitivity	377:397	detection sensitivity	377:397	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	5	42	dep	isomer-specific	1050:1064	arg1	quantitative					1067:1078	quantitative	1067:1078	quantitative	1067:1078	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	4	43	theme	isomers	863:869	arg1	quantification					798:811	quantification	798:811	quantification	798:811	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	43	theme	isomers	863:869	arg1	identification					779:792	detailed structural identification	759:792	detailed structural identification	759:792	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	2	44	from	level	271:275	arg1	quantification					223:236	accurate quantification	214:236	accurate quantification of sialylglycans at the isomeric level	214:275	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	3	45	theme	glycoqueuing	449:460	arg1	"					461:461	glycoqueuing"	449:461	glycoqueuing"	449:461	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	3	46	theme	liquid	674:679	arg1	chromatography					681:694	online reversed-phase high performance liquid chromatography	635:694	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	2	47	theme	sialylglycans	241:253	arg1	quantification					223:236	accurate quantification	214:236	accurate quantification of sialylglycans at the isomeric level	214:275	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	3	48	theme	chromatography	681:694	arg1	coupling					696:703	online reversed-phase high performance liquid chromatography coupling	635:703	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	49	theme	N-glycans	1105:1113	arg1	comparison					1080:1089	isomer-specific, quantitative comparison	1050:1089	isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1050:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	2	50	theme	mass	354:357	arg1	MS					373:374	MS	373:374	MS	373:374	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	2	50	theme	mass	354:357	arg1	spectrometry					359:370	low mass spectrometry	350:370	low mass spectrometry (MS)	350:375	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	5	51	theme	hepatocellular	1233:1246	arg1	carcinoma					1248:1256	hepatocellular carcinoma	1233:1256	hepatocellular carcinoma	1233:1256	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	2	52	theme	accurate	214:221	arg1	quantification					223:236	accurate quantification	214:236	accurate quantification of sialylglycans at the isomeric level	214:275	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	2	53	theme	low	350:352	arg1	MS					373:374	MS	373:374	MS	373:374	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	2	53	theme	low	350:352	arg1	spectrometry					359:370	low mass spectrometry	350:370	low mass spectrometry (MS)	350:375	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	5	54	theme	great	1361:1365	arg1	applicability					1367:1379	the great applicability	1357:1379	the great applicability	1357:1379	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	55	theme	carcinoma	1248:1256	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	1	56	with	related	177:183	arg1	diseases					195:202	some diseases	190:202	some diseases	190:202	Changes of α-2,3-/α-2,6-linked sialic acids (SAs) in sialylglycans have been found to be closely related with some diseases.					
31329418	3	57	theme	high	657:660	arg1	chromatography					681:694	online reversed-phase high performance liquid chromatography	635:694	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	0	58	dep	Quantification	30:43	arg1	Glycoqueuing					0:11	Glycoqueuing	0:11	Glycoqueuing	0:11	Glycoqueuing: Isomer-Specific Quantification for Sialylation-Focused Glycomics.					
31329418	4	59	theme	sialylglycan	850:861	arg1	isomers					863:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	fetal bovine serum (FBS) N-linked sialylglycan isomers	816:869	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	4	60	theme	detailed	759:766	arg1	identification					779:792	detailed structural identification	759:792	detailed structural identification	759:792	The new method was validated by detailed structural identification and quantification of fetal bovine serum (FBS) N-linked sialylglycan isomers, during which many branching isomers were successfully differentiated, and 28 sialylglycan compositions with Neu5Gc residues were analyzed.					
31329418	5	61	theme	α-2,6-sialylated	1290:1305	arg1	N-glycans					1319:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	62	theme	various	554:560	arg1	isomers					575:581	various sialylglycan isomers	554:581	various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	554:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	63	theme	rabbit	1134:1139	arg1	IgG					1159:1161	IgG	1159:1161	IgG	1159:1161	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	63	theme	rabbit	1134:1139	arg1	G					1156:1156	bovine and rabbit immunoglobulin G	1123:1156	bovine and rabbit immunoglobulin G (IgG)	1123:1162	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	64	theme	performance	662:672	arg1	chromatography					681:694	online reversed-phase high performance liquid chromatography	635:694	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	65	theme	sialylated	1094:1103	arg1	N-glycans					1105:1113	sialylated N-glycans	1094:1113	sialylated N-glycans	1094:1113	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	66	theme	sequential	477:486	arg1	elution					504:510	sequential chromatographic elution	477:510	sequential chromatographic elution	477:510	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	67	theme	fucosylated	1307:1317	arg1	N-glycans					1319:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	α-2,6-sialylated fucosylated N-glycans	1290:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	68	theme	immunoglobulin	1141:1154	arg1	IgG					1159:1161	IgG	1159:1161	IgG	1159:1161	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	68	theme	immunoglobulin	1141:1154	arg1	G					1156:1156	bovine and rabbit immunoglobulin G	1123:1156	bovine and rabbit immunoglobulin G (IgG)	1123:1162	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	2	69	theme	isomeric	262:269	arg1	level					271:275	the isomeric level	258:275	the isomeric level	258:275	However, accurate quantification of sialylglycans at the isomeric level remains challenging due to their instability, structural complexity, and low mass spectrometry (MS) detection sensitivity.					
31329418	3	70	theme	MS	533:534	arg1	quantification					536:549	high-sensitivity MS quantification	516:549	high-sensitivity MS quantification	516:549	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31329418	5	71	theme	potential	1385:1393	arg1	usage					1404:1408	potential clinical usage	1385:1408	potential clinical usage of the method	1385:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	72	theme	N-glycans	1319:1327	arg1	increase					1278:1285	a 55% increase	1272:1285	a 55% increase of α-2,6-sialylated fucosylated N-glycans	1272:1327	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	73	theme	isomer-specific	1050:1064	arg1	comparison					1080:1089	isomer-specific, quantitative comparison	1050:1089	isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1050:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	5	74	theme	serum	1183:1187	arg1	candidates					1219:1228	serum sialylated N-glycan biomarker candidates	1183:1228	serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method	1183:1422	The method was successfully applied to isomer-specific, quantitative comparison of sialylated N-glycans between bovine and rabbit immunoglobulin G (IgG) and the search for serum sialylated N-glycan biomarker candidates of hepatocellular carcinoma, during which a 55% increase of α-2,6-sialylated fucosylated N-glycans was revealed, demonstrating the great applicability and potential clinical usage of the method.					
31329418	3	75	theme	reversed-phase	642:655	arg1	chromatography					681:694	online reversed-phase high performance liquid chromatography	635:694	online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS)	635:724	Herein, we propose an analytical strategy named "glycoqueuing", which allows sequential chromatographic elution and high-sensitivity MS quantification of various sialylglycan isomers based on isotopic labeling followed by analysis via online reversed-phase high performance liquid chromatography coupling with MS (RP-HPLC-MS).					
31245822	8	0	theme	complex	1163:1169	arg1	structures					1171:1180	complex structures	1163:1180	complex structures	1163:1180	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	3	1	attach	present	411:417	arg2	glycans					403:409	glycans	403:409	glycans present in saliva	403:427	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	3	1	attach	present	411:417	arg1	saliva					422:427	saliva	422:427	saliva	422:427	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	2	2	theme	quantitative	293:304	arg1	tools					306:310	more sensitive and quantitative tools	274:310	more sensitive and quantitative tools	274:310	This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC.					
31245822	4	3	theme	PNGase	531:536	arg1	digestion					550:558	PNGase F enzymatic digestion	531:558	PNGase F enzymatic digestion	531:558	Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion.					
31245822	5	4	theme	anion	609:613	arg1	chromatography					624:637	weak anion exchange chromatography	604:637	weak anion exchange chromatography	604:637	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	5	4	theme	anion	609:613	arg1	digestions					655:664	exoglycosidase digestions	640:664	exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry	640:745	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	6	5	theme	performance	883:893	arg1	HILIC-UPLC					918:927	HILIC-UPLC	918:927	HILIC-UPLC	918:927	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	6	5	theme	performance	883:893	arg1	chromatography					902:915	Hydrophilic interaction ultra performance liquid chromatography	853:915	Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC)	853:928	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	5	6	theme	resulting	565:573	arg1	N-glycans					575:583	The resulting N-glycans	561:583	The resulting N-glycans	561:583	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	3	7	from	present	411:417	arg1	saliva					422:427	saliva	422:427	saliva	422:427	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	1	8	theme	routine	131:137	arg1	tests					139:143	routine tests	131:143	routine tests for the detection of precancerous lesions	131:185	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	9	9	used	used	1413:1416	arg2	testing					1361:1367	Mann-Whitney testing	1348:1367	Mann-Whitney testing	1348:1367	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	9	9	used	used	1413:1416	arg2	PCA					1403:1405	PCA	1403:1405	PCA	1403:1405	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	9	9	used	used	1413:1416	arg2	analysis					1393:1400	principle component analysis	1373:1400	principle component analysis (PCA)	1373:1406	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	6	10	theme	interaction	865:875	arg1	HILIC-UPLC					918:927	HILIC-UPLC	918:927	HILIC-UPLC	918:927	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	6	10	theme	interaction	865:875	arg1	chromatography					902:915	Hydrophilic interaction ultra performance liquid chromatography	853:915	Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC)	853:928	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	3	11	from	saliva	422:427	arg1	present					411:417	present	411:417	present	411:417	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	2	12	theme	unmet	259:263	arg1	need					265:268	a current unmet need	249:268	a current unmet need for more sensitive and quantitative tools for the management of OC	249:335	This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC.					
31245822	8	13	theme	mono	1216:1219	arg1	fractions					1203:1211	smaller fractions	1195:1211	smaller fractions of mono and di-sialylated structures	1195:1248	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	12	14	theme	carcinogenic	1776:1787	arg1	process					1789:1795	the carcinogenic process	1772:1795	the carcinogenic process	1772:1795	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	1	15	from	setting	219:225	arg1	routine					195:201	routine	195:201	routine	195:201	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	8	16	theme	di-sialylated	1225:1237	arg1	structures					1239:1248	di-sialylated structures	1225:1248	di-sialylated structures	1225:1248	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	6	17	theme	relative	951:958	arg1	quantitation					960:971	peak-based area relative quantitation	935:971	peak-based area relative quantitation	935:971	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	5	18	theme	ultra-high	677:686	arg1	chromatography					707:720	ultra-high performance liquid chromatography	677:720	ultra-high performance liquid chromatography	677:720	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	7	19	theme	N-glycomic	1021:1030	arg1	profile					1032:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile was identified.					
31245822	1	20	theme	precancerous	166:177	arg1	lesions					179:185	precancerous lesions	166:185	precancerous lesions	166:185	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	6	21	theme	peak-based	935:944	arg1	quantitation					960:971	peak-based area relative quantitation	935:971	peak-based area relative quantitation	935:971	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	1	22	from	routine	195:201	arg1	setting					219:225	the clinical setting	206:225	the clinical setting	206:225	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	9	23	theme	N-glycosylation	1280:1294	arg1	patterns					1296:1303	differential N-glycosylation patterns	1267:1303	differential N-glycosylation patterns	1267:1303	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	7	24	theme	complex	1004:1010	arg1	profile					1032:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile was identified.					
31245822	5	25	theme	exoglycosidase	640:653	arg1	chromatography					624:637	weak anion exchange chromatography	604:637	weak anion exchange chromatography	604:637	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	5	25	theme	exoglycosidase	640:653	arg1	digestions					655:664	exoglycosidase digestions	640:664	exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry	640:745	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	7	26	theme	abundant	991:998	arg1	profile					1032:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile was identified.					
31245822	11	27	from	active	1602:1607	arg1	process					1634:1640	the oral carcinogenic process	1612:1640	the oral carcinogenic process	1612:1640	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	9	28	theme	OC	1325:1326	arg1	groups					1340:1345	OC and control groups	1325:1345	groups	1340:1345	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	8	29	from	saliva	1087:1092	arg1	present					1076:1082	present	1076:1082	present	1076:1082	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	0	30	theme	pilot	58:62	arg1	study					64:68	A pilot study	56:68	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.	0:69	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.					
31245822	9	31	theme	control	1332:1338	arg1	groups					1340:1345	OC and control groups	1325:1345	groups	1340:1345	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	5	32	theme	liquid	700:705	arg1	chromatography					707:720	ultra-high performance liquid chromatography	677:720	ultra-high performance liquid chromatography	677:720	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	8	33	theme	main	1060:1063	arg1	structures					1065:1074	The main structures	1056:1074	The main structures present in saliva	1056:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	33	theme	main	1060:1063	arg1	oligosaccharides					1107:1122	neutral oligosaccharides	1099:1122	neutral oligosaccharides consisting of high mannose, hybrid and complex structures	1099:1180	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	0	34	theme	Salivary	0:7	arg1	N-glycosylation					9:23	Salivary N-glycosylation	0:23	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.	0:69	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.					
31245822	9	35	theme	principle	1373:1381	arg1	analysis					1393:1400	principle component analysis	1373:1400	principle component analysis (PCA)	1373:1406	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	9	35	theme	principle	1373:1381	arg1	PCA					1403:1405	PCA	1403:1405	PCA	1403:1405	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	8	36	theme	present	1076:1082	arg1	structures					1065:1074	The main structures	1056:1074	The main structures present in saliva	1056:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	36	theme	present	1076:1082	arg1	oligosaccharides					1107:1122	neutral oligosaccharides	1099:1122	neutral oligosaccharides consisting of high mannose, hybrid and complex structures	1099:1180	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	1	37	theme	Reliable	71:78	arg1	biomarkers					80:89	Reliable biomarkers	71:89	Reliable biomarkers for oral cancer (OC)	71:110	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	6	38	theme	Hydrophilic	853:863	arg1	HILIC-UPLC					918:927	HILIC-UPLC	918:927	HILIC-UPLC	918:927	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	6	38	theme	Hydrophilic	853:863	arg1	chromatography					902:915	Hydrophilic interaction ultra performance liquid chromatography	853:915	Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC)	853:928	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	4	39	theme	whole	500:504	arg1	saliva					506:511	whole saliva	500:511	whole saliva	500:511	Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion.					
31245822	10	40	dep	significant	1463:1473	arg1	P ≤ 0.05					1476:1483	P ≤ 0.05	1476:1483	P ≤ 0.05	1476:1483	Eleven peaks were shown to be statistically significant (P ≤ 0.05), while PCA analysis showed segregation of the two groups based on their glycan profile.					
31245822	8	41	theme	neutral	1099:1105	arg1	structures					1065:1074	The main structures	1056:1074	The main structures present in saliva	1056:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	41	theme	neutral	1099:1105	arg1	oligosaccharides					1107:1122	neutral oligosaccharides	1099:1122	neutral oligosaccharides consisting of high mannose, hybrid and complex structures	1099:1180	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	3	42	theme	Whole	338:342	arg1	saliva					344:349	Whole saliva	338:349	Whole saliva	338:349	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	11	43	theme	carcinogenic	1621:1632	arg1	process					1634:1640	the oral carcinogenic process	1612:1640	the oral carcinogenic process	1612:1640	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	0	44	theme	oral	43:46	arg1	cancer					48:53	oral cancer	43:53	oral cancer	43:53	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.					
31245822	10	45	theme	groups	1536:1541	arg1	segregation					1513:1523	segregation	1513:1523	segregation of the two groups based on their glycan profile	1513:1571	Eleven peaks were shown to be statistically significant (P ≤ 0.05), while PCA analysis showed segregation of the two groups based on their glycan profile.					
31245822	3	46	theme	present	411:417	arg1	glycans					403:409	glycans	403:409	glycans present in saliva	403:427	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	1	47	theme	lesions	179:185	arg1	detection					153:161	the detection	149:161	the detection of precancerous lesions	149:185	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	8	48	theme	high	1138:1141	arg1	mannose					1143:1149	high mannose	1138:1149	high mannose	1138:1149	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	6	49	theme	cancerous	810:818	arg1	lesions					825:831	cancerous oral lesions	810:831	cancerous oral lesions	810:831	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	4	50	theme	F	538:538	arg1	digestion					550:558	PNGase F enzymatic digestion	531:558	PNGase F enzymatic digestion	531:558	Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion.					
31245822	10	51	theme	glycan	1558:1563	arg1	profile					1565:1571	their glycan profile	1552:1571	their glycan profile	1552:1571	Eleven peaks were shown to be statistically significant (P ≤ 0.05), while PCA analysis showed segregation of the two groups based on their glycan profile.					
31245822	11	52	theme	early	1674:1678	arg1	detection					1680:1688	early detection	1674:1688	early detection	1674:1688	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	12	53	from	use	1815:1817	arg1	prognosis					1837:1845	prognosis	1837:1845	prognosis	1837:1845	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	12	53	from	use	1815:1817	arg1	detection					1826:1834	detection	1826:1834	detection	1826:1834	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	12	53	from	use	1815:1817	arg1	treatment					1851:1859	treatment	1851:1859	treatment	1851:1859	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	5	54	theme	weak	604:607	arg1	chromatography					624:637	weak anion exchange chromatography	604:637	weak anion exchange chromatography	604:637	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	5	54	theme	weak	604:607	arg1	digestions					655:664	exoglycosidase digestions	640:664	exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry	640:745	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	1	55	theme	clinical	210:217	arg1	setting					219:225	the clinical setting	206:225	the clinical setting	206:225	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	6	56	theme	liquid	895:900	arg1	HILIC-UPLC					918:927	HILIC-UPLC	918:927	HILIC-UPLC	918:927	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	6	56	theme	liquid	895:900	arg1	chromatography					902:915	Hydrophilic interaction ultra performance liquid chromatography	853:915	Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC)	853:928	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	3	57	theme	OC	462:463	arg1	biomarkers					465:474	OC biomarkers	462:474	OC biomarkers	462:474	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	11	58	from	process	1634:1640	arg1	active					1602:1607	active	1602:1607	active	1602:1607	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	6	59	theme	ultra	877:881	arg1	HILIC-UPLC					918:927	HILIC-UPLC	918:927	HILIC-UPLC	918:927	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	6	59	theme	ultra	877:881	arg1	chromatography					902:915	Hydrophilic interaction ultra performance liquid chromatography	853:915	Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC)	853:928	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	8	60	gly	di-sialylated	1225:1237	arg1	structures					1239:1248	di-sialylated structures	1225:1248	di-sialylated structures	1225:1248	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	61	theme	smaller	1195:1201	arg1	fractions					1203:1211	smaller fractions	1195:1211	smaller fractions of mono and di-sialylated structures	1195:1248	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	6	62	theme	N-glycan	761:768	arg1	changes					770:776	N-glycan changes	761:776	N-glycan changes	761:776	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	2	63	theme	current	251:257	arg1	need					265:268	a current unmet need	249:268	a current unmet need for more sensitive and quantitative tools for the management of OC	249:335	This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC.					
31245822	6	64	theme	area	946:949	arg1	quantitation					960:971	peak-based area relative quantitation	935:971	peak-based area relative quantitation	935:971	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	11	65	theme	N-glycosylation	1574:1588	arg1	changes					1590:1596	N-glycosylation changes	1574:1596	N-glycosylation changes	1574:1596	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	11	65	theme	N-glycosylation	1574:1588	arg1	biomarkers					1659:1668	biomarkers	1659:1668	biomarkers for early detection	1659:1688	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	5	66	theme	exchange	615:622	arg1	chromatography					624:637	weak anion exchange chromatography	604:637	weak anion exchange chromatography	604:637	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	5	66	theme	exchange	615:622	arg1	digestions					655:664	exoglycosidase digestions	640:664	exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry	640:745	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	9	67	theme	differential	1267:1278	arg1	patterns					1296:1303	differential N-glycosylation patterns	1267:1303	differential N-glycosylation patterns	1267:1303	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	1	68	theme	oral	95:98	arg1	OC					108:109	OC	108:109	OC	108:109	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	1	68	theme	oral	95:98	arg1	cancer					100:105	oral cancer	95:105	oral cancer (OC)	95:110	Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting.					
31245822	8	69	theme	structures	1239:1248	arg1	fractions					1203:1211	smaller fractions	1195:1211	smaller fractions of mono and di-sialylated structures	1195:1248	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	7	70	theme	salivary	1012:1019	arg1	profile					1032:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile	988:1038	An abundant and complex salivary N-glycomic profile was identified.					
31245822	5	71	theme	mass	729:732	arg1	spectrometry					734:745	mass spectrometry	729:745	mass spectrometry	729:745	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	5	72	theme	performance	688:698	arg1	chromatography					707:720	ultra-high performance liquid chromatography	677:720	ultra-high performance liquid chromatography	677:720	The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry.					
31245822	12	73	dep	detection	1826:1834	arg1	the					1822:1824	the	1822:1824	the	1822:1824	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	9	74	theme	Mann-Whitney	1348:1359	arg1	testing					1361:1367	Mann-Whitney testing	1348:1367	Mann-Whitney testing	1348:1367	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	10	75	theme	PCA	1493:1495	arg1	analysis					1497:1504	PCA analysis	1493:1504	PCA analysis	1493:1504	Eleven peaks were shown to be statistically significant (P ≤ 0.05), while PCA analysis showed segregation of the two groups based on their glycan profile.					
31245822	2	76	theme	OC	334:335	arg1	management					320:329	the management	316:329	the management of OC	316:335	This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC.					
31245822	9	77	theme	component	1383:1391	arg1	analysis					1393:1400	principle component analysis	1373:1400	principle component analysis (PCA)	1373:1406	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	9	77	theme	component	1383:1391	arg1	PCA					1403:1405	PCA	1403:1405	PCA	1403:1405	To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann-Whitney testing and principle component analysis (PCA) were used.					
31245822	3	78	theme	glycans	403:409	arg1	nature					393:398	the nature	389:398	the nature of glycans present in saliva	389:427	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	8	79	attach	present	1076:1082	arg2	structures					1065:1074	The main structures	1056:1074	The main structures present in saliva	1056:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	79	attach	present	1076:1082	arg2	oligosaccharides					1107:1122	neutral oligosaccharides	1099:1122	neutral oligosaccharides consisting of high mannose, hybrid and complex structures	1099:1180	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	8	79	attach	present	1076:1082	arg1	saliva					1087:1092	saliva	1087:1092	saliva	1087:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31245822	11	80	theme	oral	1616:1619	arg1	process					1634:1640	the oral carcinogenic process	1612:1640	the oral carcinogenic process	1612:1640	N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality.					
31245822	0	81	theme	cancer	48:53	arg1	biomarker					30:38	a biomarker	28:38	a biomarker of oral cancer	28:53	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.					
31245822	12	82	theme	OC	1864:1865	arg1	prognosis					1837:1845	prognosis	1837:1845	prognosis	1837:1845	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	12	82	theme	OC	1864:1865	arg1	detection					1826:1834	detection	1826:1834	detection	1826:1834	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	12	82	theme	OC	1864:1865	arg1	treatment					1851:1859	treatment	1851:1859	treatment	1851:1859	Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.					
31245822	2	83	theme	sensitive	279:287	arg1	tools					306:310	more sensitive and quantitative tools	274:310	more sensitive and quantitative tools	274:310	This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC.					
31245822	3	84	used	used	355:358	arg2	saliva					344:349	Whole saliva	338:349	Whole saliva	338:349	Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers.					
31245822	6	85	theme	oral	820:823	arg1	lesions					825:831	cancerous oral lesions	810:831	cancerous oral lesions	810:831	To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC), and peak-based area relative quantitation was performed.					
31245822	4	86	theme	enzymatic	540:548	arg1	digestion					550:558	PNGase F enzymatic digestion	531:558	PNGase F enzymatic digestion	531:558	Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion.					
31245822	0	87	dep	N-glycosylation	9:23	arg1	study					64:68	A pilot study	56:68	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.	0:69	Salivary N-glycosylation as a biomarker of oral cancer: A pilot study.					
31245822	8	88	from	present	1076:1082	arg1	saliva					1087:1092	saliva	1087:1092	saliva	1087:1092	The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures.					
31640540	7	0	theme	glycosylation	1398:1410	arg1	state					1412:1416	the glycosylation state	1394:1416	the glycosylation state of all the sequons on a protein structure	1394:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	7	1	from	sequons	1429:1435	arg1	structure					1450:1458	a protein structure	1440:1458	a protein structure	1440:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	11	2	theme	force	1872:1876	arg1	field					1878:1882	the CHARMM force field	1861:1882	the CHARMM force field	1861:1882	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	4	3	dep	GLYCAM-WEB	679:688	arg1	e.g.					673:676	e.g.	673:676	e.g.	673:676	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	4	3	dep	GLYCAM-WEB	679:688	arg1	http					691:694	http	691:694	http:// https://dev.glycam.org/gp/	691:724	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	2	4	link	N-linked	324:331	arg1	glycosylation					333:345	N-linked glycosylation	324:345	N-linked glycosylation	324:345	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	2	5	theme	nitrogen	267:274	arg1	atom					276:279	the nitrogen atom	263:279	the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation,	263:346	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	7	6	gly	glycoprotein	1336:1347	arg1	glycoprotein					1336:1347	a glycoprotein	1334:1347	a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure	1334:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	7	7	theme	visual	1373:1378	arg1	inspection					1380:1389	a quick visual inspection	1365:1389	a quick visual inspection of the glycosylation state of all the sequons on a protein structure	1365:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	4	8	theme	tools	626:630	arg1	number					598:603	a number	596:603	a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ ))	596:780	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	6	9	theme	programming	1190:1200	arg1	API					1213:1215	API	1213:1215	API	1213:1215	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	9	theme	programming	1190:1200	arg1	interface					1202:1210	its application programming interface	1174:1210	its application programming interface (API)	1174:1216	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	3	10	from	modeling	441:448	arg1	computer					472:479	a personal desktop computer	453:479	a personal desktop computer	453:479	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	3	11	theme	molecular	503:511	arg1	dynamics					513:520	molecular dynamics	503:520	molecular dynamics to interface with specialized programs such as CHARMM or AMBER	503:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	2	12	theme	asparagine	287:296	arg1	atom					276:279	the nitrogen atom	263:279	the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation,	263:346	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	9	13	theme	specific	1615:1622	arg1	glycans					1647:1653	specific three-dimensional (3D) glycans	1615:1653	specific three-dimensional (3D) glycans	1615:1653	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	12	14	theme	modeling	2033:2040	arg1	glycans					2042:2048	modeling glycans	2033:2048	modeling glycans than any other available software	2033:2082	Glycosylator provides more functionality for analyzing and modeling glycans than any other available software or webserver at present.					
31640540	1	15	theme	large	180:184	arg1	structures					209:218	large multi-branching glycan structures	180:218	large multi-branching glycan structures	180:218	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	11	16	theme	code	1968:1971	arg1	internals					1951:1959	the internals	1947:1959	the internals of the code	1947:1971	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	4	17	theme	popular	608:614	arg1	tools					626:630	popular web-based tools	608:630	popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ ))	608:780	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	11	18	theme	new	1885:1887	arg1	moieties					1903:1910	new complex sugar moieties	1885:1910	new complex sugar moieties	1885:1910	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	1	19	theme	glycan	202:207	arg1	structures					209:218	large multi-branching glycan structures	180:218	large multi-branching glycan structures	180:218	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	6	20	theme	Python	1152:1157	arg1	script					1159:1164	a Python script	1150:1164	a Python script	1150:1164	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	21	theme	graphical	1233:1241	arg1	GUI					1259:1261	GUI	1259:1261	GUI	1259:1261	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	21	theme	graphical	1233:1241	arg1	interface					1248:1256	its graphical user interface	1229:1256	its graphical user interface (GUI)	1229:1262	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	5	22	theme	glycan	855:860	arg1	conformations					862:874	a few canonical glycan conformations	839:874	a few canonical glycan conformations	839:874	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	4	23	dep	http	691:694	arg1	//dev.glycam.org/gp/					705:724	//dev.glycam.org/gp/	705:724	//dev.glycam.org/gp/	705:724	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	4	23	dep	http	691:694	arg1	https					699:703	// https	696:703	http:// https://dev.glycam.org/gp/	691:724	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	9	24	theme	protein	1660:1666	arg1	structure					1668:1676	a protein structure	1658:1676	a protein structure using a library of predefined templates	1658:1716	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	11	25	theme	sugar	1897:1901	arg1	moieties					1903:1910	new complex sugar moieties	1885:1910	new complex sugar moieties	1885:1910	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	1	26	theme	biomolecules	126:137	arg1	Carbohydrates					79:91	BACKGROUND Carbohydrates	68:91	BACKGROUND Carbohydrates	68:91	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	1	26	theme	biomolecules	126:137	arg1	class					99:103	a class	97:103	a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures	97:218	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	0	27	theme	glycans	59:65	arg1	modeling					47:54	the rapid modeling	37:54	the rapid modeling of glycans	37:65	Glycosylator: a Python framework for the rapid modeling of glycans.					
31640540	5	28	theme	few	841:843	arg1	conformations					862:874	a few canonical glycan conformations	839:874	a few canonical glycan conformations	839:874	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	9	29	theme	predefined	1697:1706	arg1	templates					1708:1716	predefined templates	1697:1716	predefined templates	1697:1716	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	11	30	theme	CHARMM	1865:1870	arg1	field					1878:1882	the CHARMM force field	1861:1882	the CHARMM force field	1861:1882	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	5	31	theme	glycan	917:922	arg1	modeling					924:931	glycan modeling	917:931	glycan modeling	917:931	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	7	32	gly	glycosylation	1398:1410	arg1	sequons					1429:1435	all the sequons	1421:1435	all the sequons on a protein structure	1421:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	2	33	theme	living	398:403	arg1	organisms					405:413	many living organisms	393:413	many living organisms	393:413	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	4	34	theme	//	696:697	arg1	https					699:703	// https	696:703	http:// https://dev.glycam.org/gp/	691:724	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	6	35	from	modification	1076:1087	arg1	structure					1111:1119	protein structure	1103:1119	protein structure	1103:1119	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	13	36	theme	modeling	2188:2195	arg1	communities					2197:2207	biomolecular modeling communities	2175:2207	biomolecular modeling communities	2175:2207	Glycosylator will be a valuable tool for the glycoinformatics and biomolecular modeling communities.					
31640540	8	37	theme	Modeled	1461:1467	arg1	glycans					1469:1475	Modeled glycans	1461:1475	Modeled glycans	1461:1475	Modeled glycans can be further refined by a genetic algorithm for removing clashes and sampling alternative conformations.					
31640540	10	38	theme	protein	1788:1794	arg1	models					1765:1770	models	1765:1770	models of glycosylated protein without steric clashes	1765:1817	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	6	39	from	glycans	1092:1098	arg1	structure					1111:1119	protein structure	1103:1119	protein structure	1103:1119	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	3	40	theme	Most	416:419	arg1	software					421:428	Most software	416:428	Most software for glycan modeling on a personal desktop computer	416:479	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	4	41	dep	http	750:753	arg1	//www.glycosciences.de/					755:777	//www.glycosciences.de/	755:777	http://www.glycosciences.de/	750:777	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	7	42	theme	sequons	1429:1435	arg1	state					1412:1416	the glycosylation state	1394:1416	the glycosylation state of all the sequons on a protein structure	1394:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	5	43	theme	modeling	962:969	arg1	workflow					971:978	their protein structure modeling workflow	938:978	their protein structure modeling workflow	938:978	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	6	44	from	identification	1047:1060	arg1	structure					1111:1119	protein structure	1103:1119	protein structure	1103:1119	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	45	from	modeling	1063:1070	arg1	structure					1111:1119	protein structure	1103:1119	protein structure	1103:1119	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	46	used	used	1133:1136	arg2	Glycosylator					1006:1017	Glycosylator	1006:1017	Glycosylator	1006:1017	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	46	used	used	1133:1136	arg2	framework					1029:1037	a Python framework	1020:1037	a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI)	1020:1262	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	7	47	theme	state	1412:1416	arg1	inspection					1380:1389	a quick visual inspection	1365:1389	a quick visual inspection of the glycosylation state of all the sequons on a protein structure	1365:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	10	48	theme	steric	1804:1809	arg1	clashes					1811:1817	steric clashes	1804:1817	steric clashes	1804:1817	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	5	49	theme	protein	944:950	arg1	workflow					971:978	their protein structure modeling workflow	938:978	their protein structure modeling workflow	938:978	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	8	50	theme	genetic	1505:1511	arg1	algorithm					1513:1521	a genetic algorithm	1503:1521	a genetic algorithm for removing clashes and sampling alternative conformations	1503:1581	Modeled glycans can be further refined by a genetic algorithm for removing clashes and sampling alternative conformations.					
31640540	2	51	theme	N-linked	324:331	arg1	glycosylation					333:345	N-linked glycosylation	324:345	N-linked glycosylation	324:345	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	6	52	theme	Python	1022:1027	arg1	Glycosylator					1006:1017	Glycosylator	1006:1017	Glycosylator	1006:1017	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	52	theme	Python	1022:1027	arg1	framework					1029:1037	a Python framework	1020:1037	a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI)	1020:1262	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	3	53	theme	dynamics	513:520	arg1	knowledge					490:498	knowledge	490:498	knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER	490:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	7	54	theme	quick	1367:1371	arg1	inspection					1380:1389	a quick visual inspection	1365:1389	a quick visual inspection of the glycosylation state of all the sequons on a protein structure	1365:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	4	55	dep	Glycosciences.db	731:746	arg1	http					750:753	http	750:753	http://www.glycosciences.de/	750:777	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	5	56	theme	web-based	798:806	arg1	tools					808:812	these web-based tools	792:812	these web-based tools	792:812	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	6	57	theme	protein	1103:1109	arg1	structure					1111:1119	protein structure	1103:1119	protein structure	1103:1119	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	4	58	theme	web-based	616:624	arg1	tools					626:630	popular web-based tools	608:630	popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ ))	608:780	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	9	59	dep	three-dimensional	1624:1640	arg1	3D					1643:1644	3D	1643:1644	3D	1643:1644	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	6	60	theme	application	1178:1188	arg1	API					1213:1215	API	1213:1215	API	1213:1215	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	60	theme	application	1178:1188	arg1	interface					1202:1210	its application programming interface	1174:1210	its application programming interface (API)	1174:1216	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	3	61	theme	specialized	540:550	arg1	CHARMM					569:574	CHARMM	569:574	CHARMM	569:574	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	3	61	theme	specialized	540:550	arg1	AMBER					579:583	AMBER	579:583	AMBER	579:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	3	61	theme	specialized	540:550	arg1	programs					552:559	specialized programs	540:559	specialized programs such as CHARMM or AMBER	540:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	7	62	dep	two-dimensional	1300:1314	arg1	2D					1317:1318	2D	1317:1318	2D	1317:1318	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	6	63	theme	glycans	1092:1098	arg1	modification					1076:1087	modification	1076:1087	modification	1076:1087	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	63	theme	glycans	1092:1098	arg1	modeling					1063:1070	modeling	1063:1070	modeling	1063:1070	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	63	theme	glycans	1092:1098	arg1	identification					1047:1060	identification	1047:1060	identification	1047:1060	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	12	64	theme	available	2065:2073	arg1	software					2075:2082	any other available software	2055:2082	any other available software	2055:2082	Glycosylator provides more functionality for analyzing and modeling glycans than any other available software or webserver at present.					
31640540	10	65	used	used	1748:1751	arg2	Glycosylator					1731:1742	CONCLUSIONS Glycosylator	1719:1742	CONCLUSIONS Glycosylator	1719:1742	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	1	66	theme	simple	155:160	arg1	monosaccharide					162:175	a simple monosaccharide	153:175	a simple monosaccharide	153:175	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	9	67	theme	three-dimensional	1624:1640	arg1	glycans					1647:1653	specific three-dimensional (3D) glycans	1615:1653	specific three-dimensional (3D) glycans	1615:1653	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	3	68	theme	desktop	464:470	arg1	computer					472:479	a personal desktop computer	453:479	a personal desktop computer	453:479	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	2	69	theme	covalent	225:232	arg1	linkage					234:240	The covalent linkage	221:240	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation,	221:346	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	1	70	theme	multi-branching	186:200	arg1	structures					209:218	large multi-branching glycan structures	180:218	large multi-branching glycan structures	180:218	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	6	71	theme	user	1243:1246	arg1	GUI					1259:1261	GUI	1259:1261	GUI	1259:1261	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	71	theme	user	1243:1246	arg1	interface					1248:1256	its graphical user interface	1229:1256	its graphical user interface (GUI)	1229:1262	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	10	72	theme	CONCLUSIONS	1719:1729	arg1	Glycosylator					1731:1742	CONCLUSIONS Glycosylator	1719:1742	CONCLUSIONS Glycosylator	1719:1742	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	5	73	theme	canonical	845:853	arg1	conformations					862:874	a few canonical glycan conformations	839:874	a few canonical glycan conformations	839:874	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	2	74	theme	carbohydrate	247:258	arg1	linkage					234:240	The covalent linkage	221:240	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation,	221:346	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	1	75	theme	large	108:112	arg1	biomolecules					126:137	large and diverse biomolecules	108:137	large and diverse biomolecules	108:137	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	11	76	theme	complex	1889:1895	arg1	moieties					1903:1910	new complex sugar moieties	1885:1910	new complex sugar moieties	1885:1910	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	1	77	theme	diverse	118:124	arg1	biomolecules					126:137	large and diverse biomolecules	108:137	large and diverse biomolecules	108:137	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	4	78	theme	modeling	655:662	arg1	glycans					664:670	modeling glycans	655:670	modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ ))	655:780	There are a number of popular web-based tools that are available for modeling glycans (e.g., GLYCAM-WEB (http:// https://dev.glycam.org/gp/ ) or Glycosciences.db ( http://www.glycosciences.de/ )).					
31640540	6	79	dep	RESULTS	981:987	arg1	present					998:1004	present	998:1004	present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI)	998:1262	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	80	from	structure	1111:1119	arg1	modification					1076:1087	modification	1076:1087	modification	1076:1087	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	80	from	structure	1111:1119	arg1	modeling					1063:1070	modeling	1063:1070	modeling	1063:1070	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	6	80	from	structure	1111:1119	arg1	identification					1047:1060	identification	1047:1060	identification	1047:1060	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	9	81	theme	templates	1708:1716	arg1	library					1686:1692	a library	1684:1692	a library of predefined templates	1684:1716	Glycosylator can also identify specific three-dimensional (3D) glycans on a protein structure using a library of predefined templates.					
31640540	2	82	theme	important	357:365	arg1	role					367:370	an important role	354:370	an important role	354:370	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	3	83	with	interface	525:533	arg1	CHARMM					569:574	CHARMM	569:574	CHARMM	569:574	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	3	83	with	interface	525:533	arg1	AMBER					579:583	AMBER	579:583	AMBER	579:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	3	83	with	interface	525:533	arg1	programs					552:559	specialized programs	540:559	specialized programs such as CHARMM or AMBER	540:583	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	1	84	theme	BACKGROUND	68:77	arg1	Carbohydrates					79:91	BACKGROUND Carbohydrates	68:91	BACKGROUND Carbohydrates	68:91	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	1	84	theme	BACKGROUND	68:77	arg1	class					99:103	a class	97:103	a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures	97:218	BACKGROUND Carbohydrates are a class of large and diverse biomolecules, ranging from a simple monosaccharide to large multi-branching glycan structures.					
31640540	2	85	theme	organisms	405:413	arg1	physiology					379:388	the physiology	375:388	the physiology of many living organisms	375:413	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	11	86	theme	molecular	1830:1838	arg1	topology					1840:1847	the molecular topology	1826:1847	the molecular topology	1826:1847	Since the molecular topology is based on the CHARMM force field, new complex sugar moieties can be generated without modifying the internals of the code.					
31640540	0	87	theme	Python	16:21	arg1	framework					23:31	a Python framework	14:31	Glycosylator: a Python framework for the rapid modeling of glycans.	0:66	Glycosylator: a Python framework for the rapid modeling of glycans.					
31640540	7	88	from	structure	1450:1458	arg1	state					1412:1416	the glycosylation state	1394:1416	the glycosylation state of all the sequons on a protein structure	1394:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	2	89	theme	many	393:396	arg1	organisms					405:413	many living organisms	393:413	many living organisms	393:413	The covalent linkage of a carbohydrate to the nitrogen atom of an asparagine, a process referred to as N-linked glycosylation, plays an important role in the physiology of many living organisms.					
31640540	3	90	theme	glycan	434:439	arg1	modeling					441:448	glycan modeling	434:448	glycan modeling on a personal desktop computer	434:479	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	13	91	theme	biomolecular	2175:2186	arg1	communities					2197:2207	biomolecular modeling communities	2175:2207	biomolecular modeling communities	2175:2207	Glycosylator will be a valuable tool for the glycoinformatics and biomolecular modeling communities.					
31640540	7	92	theme	protein	1442:1448	arg1	structure					1450:1458	a protein structure	1440:1458	a protein structure	1440:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	5	93	theme	structure	952:960	arg1	workflow					971:978	their protein structure modeling workflow	938:978	their protein structure modeling workflow	938:978	However, these web-based tools are generally limited to a few canonical glycan conformations and do not allow the user to incorporate glycan modeling into their protein structure modeling workflow.					
31640540	10	94	theme	glycosylated	1775:1786	arg1	protein					1788:1794	glycosylated protein	1775:1794	glycosylated protein without steric clashes	1775:1817	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	0	95	theme	rapid	41:45	arg1	modeling					47:54	the rapid modeling	37:54	the rapid modeling of glycans	37:65	Glycosylator: a Python framework for the rapid modeling of glycans.					
31640540	13	96	theme	valuable	2132:2139	arg1	tool					2141:2144	a valuable tool	2130:2144	a valuable tool for the glycoinformatics and biomolecular modeling communities	2130:2207	Glycosylator will be a valuable tool for the glycoinformatics and biomolecular modeling communities.					
31640540	13	96	theme	valuable	2132:2139	arg1	Glycosylator					2109:2120	Glycosylator	2109:2120	Glycosylator	2109:2120	Glycosylator will be a valuable tool for the glycoinformatics and biomolecular modeling communities.					
31640540	12	97	theme	other	2059:2063	arg1	software					2075:2082	any other available software	2055:2082	any other available software	2055:2082	Glycosylator provides more functionality for analyzing and modeling glycans than any other available software or webserver at present.					
31640540	3	98	theme	personal	455:462	arg1	computer					472:479	a personal desktop computer	453:479	a personal desktop computer	453:479	Most software for glycan modeling on a personal desktop computer requires knowledge of molecular dynamics to interface with specialized programs such as CHARMM or AMBER.					
31640540	8	99	theme	alternative	1557:1567	arg1	conformations					1569:1581	alternative conformations	1557:1581	alternative conformations	1557:1581	Modeled glycans can be further refined by a genetic algorithm for removing clashes and sampling alternative conformations.					
31640540	0	100	dep	Glycosylator	0:11	arg1	framework					23:31	a Python framework	14:31	Glycosylator: a Python framework for the rapid modeling of glycans.	0:66	Glycosylator: a Python framework for the rapid modeling of glycans.					
31640540	6	101	dep	identification	1047:1060	arg1	the					1043:1045	the	1043:1045	the	1043:1045	RESULTS Here, we present Glycosylator, a Python framework for the identification, modeling and modification of glycans in protein structure that can be used directly in a Python script through its application programming interface (API) or through its graphical user interface (GUI).					
31640540	7	102	from	state	1412:1416	arg1	structure					1450:1458	a protein structure	1440:1458	a protein structure	1440:1458	The GUI provides a straightforward two-dimensional (2D) rendering of a glycoprotein that allows for a quick visual inspection of the glycosylation state of all the sequons on a protein structure.					
31640540	10	103	gly	glycosylated	1775:1786	arg1	protein					1788:1794	glycosylated protein	1775:1794	glycosylated protein without steric clashes	1775:1817	CONCLUSIONS Glycosylator was used to generate models of glycosylated protein without steric clashes.					
31640540	12	104	from	present	2100:2106	arg1	glycans					2042:2048	modeling glycans	2033:2048	modeling glycans than any other available software	2033:2082	Glycosylator provides more functionality for analyzing and modeling glycans than any other available software or webserver at present.					
31640540	12	104	from	present	2100:2106	arg1	webserver					2087:2095	webserver	2087:2095	webserver at present	2087:2106	Glycosylator provides more functionality for analyzing and modeling glycans than any other available software or webserver at present.					
30104209	1	0	theme	biological	394:403	arg1	effects					405:411	various biological effects	386:411	various biological effects	386:411	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	2	1	theme	glycan	543:548	arg1	structures					550:559	the glycan structures	539:559	the glycan structures previously defined	539:578	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	4	2	theme	N-glycans	1024:1032	arg1	β-mannosylation					1001:1015	core β-mannosylation	996:1015	core β-mannosylation of the N-glycans	996:1032	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	2	theme	N-glycans	1024:1032	arg1	sulfation					962:970	sulfation	962:970	sulfation of mannose residues	962:990	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	2	theme	N-glycans	1024:1032	arg1	termini					953:959	previously overlooked glucuronic acid termini	915:959	previously overlooked glucuronic acid termini	915:959	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	3	3	theme	royal	681:685	arg1	jelly					687:691	whole royal jelly	675:691	whole royal jelly as well as single protein bands	675:723	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	4	theme	bee	1440:1442	arg1	venom					1444:1448	even bee venom	1435:1448	even bee venom	1435:1448	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	5	5	theme	insect	1417:1422	arg1	samples					1424:1430	other insect samples	1411:1430	other insect samples	1411:1430	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	6	theme	queen	189:193	arg1	honeybees					195:203	queen honeybees	189:203	queen honeybees as well as claims of benefits on human health	189:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	7	theme	other	893:897	arg1	species					906:912	other insect species	893:912	other insect species	893:912	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	8	theme	rare	1073:1076	arg1	present					1132:1138	present	1132:1138	present	1132:1138	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	8	theme	rare	1073:1076	arg1	modification					1091:1102	a relatively rare zwitterionic modification	1060:1102	a relatively rare zwitterionic modification with phosphoethanolamine	1060:1127	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	1	9	from	health	244:249	arg1	development					174:184	the development	170:184	the development of queen honeybees as well as claims of benefits on human health	170:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	10	from	studies	882:888	arg1	species					906:912	other insect species	893:912	other insect species	893:912	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	3	11	from	study	668:672	arg1	subject					730:736	subject	730:736	subject	730:736	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	3	12	theme	protein	711:717	arg1	bands					719:723	single protein bands	704:723	whole royal jelly as well as single protein bands	675:723	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	13	from	remodelling	1243:1253	arg1	apparatus					1279:1287	the Golgi apparatus	1269:1287	the Golgi apparatus of hypopharyngeal gland cells	1269:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	14	from	honeybees	195:203	arg1	health					244:249	human health	238:249	human health	238:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	15	theme	recent	866:871	arg1	studies					882:888	recent in-depth studies	866:888	recent in-depth studies on other insect species	866:912	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	3	16	theme	LC-MALDI-TOF	750:761	arg1	analyses					775:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	6	17	theme	honeybee	1572:1579	arg1	development					1581:1591	honeybee development	1572:1591	honeybee development	1572:1591	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	4	18	theme	glucuronic	937:946	arg1	termini					953:959	previously overlooked glucuronic acid termini	915:959	previously overlooked glucuronic acid termini	915:959	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	0	19	from	Glycoproteins	91:103	arg1	Recognition					24:34	Recognition	24:34	Recognition	24:34	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	0	19	from	Glycoproteins	91:103	arg1	Separation					9:18	Isomeric Separation	0:18	Isomeric Separation	0:18	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	5	20	theme	hypopharyngeal	1292:1305	arg1	cells					1313:1317	hypopharyngeal gland cells	1292:1317	hypopharyngeal gland cells	1292:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	0	21	theme	N-glycans	64:72	arg1	Recognition					24:34	Recognition	24:34	Recognition	24:34	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	0	21	theme	N-glycans	64:72	arg1	Separation					9:18	Isomeric Separation	0:18	Isomeric Separation	0:18	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	3	22	theme	arraying	838:845	arg1	data					847:850	arraying data	838:850	arraying data	838:850	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	23	theme	glycans	1258:1264	arg1	remodelling					1243:1253	tissue-specific remodelling	1227:1253	tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells	1227:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	0	24	theme	Royal	79:83	arg1	Glycoproteins					91:103	Royal Jelly Glycoproteins	79:103	Royal Jelly Glycoproteins	79:103	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	1	25	theme	large	320:324	arg1	number					326:331	a large number	318:331	a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state	318:444	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	6	26	theme	animal	1656:1661	arg1	immunity					1670:1677	animal innate immunity	1656:1677	animal innate immunity	1656:1677	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	3	27	theme	glycomic	766:773	arg1	analyses					775:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	28	theme	low	1327:1329	arg1	glycans					1372:1378	fucosylated or paucimannosidic glycans	1341:1378	glycans	1372:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	5	28	theme	low	1327:1329	arg1	amount					1331:1336	only a low amount	1320:1336	only a low amount of fucosylated or paucimannosidic glycans	1320:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	0	29	theme	Isomeric	0:7	arg1	Separation					9:18	Isomeric Separation	0:18	Isomeric Separation	0:18	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	6	30	theme	innate	1663:1668	arg1	immunity					1670:1677	animal innate immunity	1656:1677	animal innate immunity	1656:1677	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	2	31	theme	specific	622:629	arg1	functions					642:650	specific biological functions	622:650	specific biological functions	622:650	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	5	32	theme	cells	1313:1317	arg1	apparatus					1279:1287	the Golgi apparatus	1269:1287	the Golgi apparatus of hypopharyngeal gland cells	1269:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	33	theme	glands	287:292	arg1	product					257:263	this product	252:263	this product of the hypopharyngeal glands of worker bees	252:307	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	34	with	modification	1091:1102	arg1	phosphoethanolamine					1109:1127	phosphoethanolamine	1109:1127	phosphoethanolamine	1109:1127	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	35	theme	mannose	975:981	arg1	residues					983:990	mannose residues	975:990	mannose residues	975:990	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	1	36	theme	worker	297:302	arg1	bees					304:307	worker bees	297:307	worker bees	297:307	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	37	gly	β-mannosylation	1001:1015	arg1	N-glycans					1024:1032	the N-glycans	1020:1032	the N-glycans	1020:1032	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	37	gly	β-mannosylation	1001:1015	arg1	residues					983:990	mannose residues	975:990	mannose residues	975:990	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	1	38	theme	claims	216:221	arg1	development					174:184	the development	170:184	the development of queen honeybees as well as claims of benefits on human health	170:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	5	39	theme	tissue-specific	1227:1241	arg1	remodelling					1243:1253	tissue-specific remodelling	1227:1253	tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells	1227:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	6	40	with	pentraxins	1631:1640	arg1	roles					1647:1651	roles	1647:1651	roles in animal innate immunity	1647:1677	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	3	41	theme	off-line	741:748	arg1	analyses					775:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	42	theme	glycans	1372:1378	arg1	glycans					1372:1378	fucosylated or paucimannosidic glycans	1341:1378	glycans	1372:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	5	42	theme	glycans	1372:1378	arg1	amount					1331:1336	only a low amount	1320:1336	only a low amount of fucosylated or paucimannosidic glycans	1320:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	6	43	theme	unusual	1455:1461	arg1	modifications					1463:1475	The unusual modifications	1451:1475	The unusual modifications of hybrid and multiantennary structures	1451:1515	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	1	44	theme	human	238:242	arg1	health					244:249	human health	238:249	human health	238:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	2	45	theme	structures	550:559	arg1	none					531:534	none	531:534	none of the glycan structures previously defined	531:578	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	6	46	dep	defined	1517:1523	arg1	may					1530:1532	may	1530:1532	may	1530:1532	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	1	47	theme	glycosylated	427:438	arg1	state					440:444	their glycosylated state	421:444	their glycosylated state	421:444	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	5	48	theme	even	1435:1438	arg1	venom					1444:1448	even bee venom	1435:1448	even bee venom	1435:1448	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	6	49	theme	physiological	1550:1562	arg1	role					1564:1567	a physiological role	1548:1567	a physiological role	1548:1567	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	6	50	dep	may	1530:1532	arg1	only					1538:1541	only	1538:1541	only	1538:1541	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	6	51	from	roles	1647:1651	arg1	immunity					1670:1677	animal innate immunity	1656:1677	animal innate immunity	1656:1677	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	2	52	contain	have	596:599	arg1	none					531:534	none	531:534	none of the glycan structures previously defined	531:578	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	2	52	contain	have	596:599	arg2	potential					601:609	potential	601:609	potential	601:609	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	3	53	theme	whole	675:679	arg1	jelly					687:691	whole royal jelly	675:691	whole royal jelly as well as single protein bands	675:723	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	54	theme	other	1411:1415	arg1	samples					1424:1430	other insect samples	1411:1430	other insect samples	1411:1430	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	2	55	theme	defined	572:578	arg1	structures					550:559	the glycan structures	539:559	the glycan structures previously defined	539:578	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	3	56	theme	single	704:709	arg1	bands					719:723	single protein bands	704:723	whole royal jelly as well as single protein bands	675:723	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	4	57	dep	phosphorylcholine	1160:1176	arg1	contrast					1144:1151	contrast	1144:1151	contrast	1144:1151	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	1	58	theme	honeybees	195:203	arg1	development					174:184	the development	170:184	the development of queen honeybees as well as claims of benefits on human health	170:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	59	theme	in-depth	873:880	arg1	studies					882:888	recent in-depth studies	866:888	recent in-depth studies on other insect species	866:912	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	60	theme	zwitterionic	1078:1089	arg1	present					1132:1138	present	1132:1138	present	1132:1138	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	60	theme	zwitterionic	1078:1089	arg1	modification					1091:1102	a relatively rare zwitterionic modification	1060:1102	a relatively rare zwitterionic modification with phosphoethanolamine	1060:1127	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	6	61	theme	structures	1506:1515	arg1	modifications					1463:1475	The unusual modifications	1451:1475	The unusual modifications of hybrid and multiantennary structures	1451:1515	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	2	62	theme	glycoproteomic	494:507	arg1	analyses					509:516	glycomic and glycoproteomic analyses	481:516	glycomic and glycoproteomic analyses	481:516	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	5	63	gly	fucosylated	1341:1351	arg1	glycans					1372:1378	fucosylated or paucimannosidic glycans	1341:1378	glycans	1372:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	5	64	theme	Golgi	1273:1277	arg1	apparatus					1279:1287	the Golgi apparatus	1269:1287	the Golgi apparatus of hypopharyngeal gland cells	1269:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	65	from	claims	216:221	arg1	health					244:249	human health	238:249	human health	238:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	3	66	theme	Western	817:823	arg1	blotting					825:832	Western blotting	817:832	Western blotting	817:832	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	3	67	theme	MS	763:764	arg1	analyses					775:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	off-line LC-MALDI-TOF MS glycomic analyses	741:782	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	1	68	theme	various	386:392	arg1	effects					405:411	various biological effects	386:411	various biological effects	386:411	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	69	theme	overlooked	926:935	arg1	termini					953:959	previously overlooked glucuronic acid termini	915:959	previously overlooked glucuronic acid termini	915:959	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	70	theme	insect	899:904	arg1	species					906:912	other insect species	893:912	other insect species	893:912	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	0	71	theme	Jelly	85:89	arg1	Glycoproteins					91:103	Royal Jelly Glycoproteins	79:103	Royal Jelly Glycoproteins	79:103	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	1	72	from	development	174:184	arg1	health					244:249	human health	238:249	human health	238:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	73	gly	glycosylated	427:438	arg1	state					440:444	their glycosylated state	421:444	their glycosylated state	421:444	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	74	theme	acid	948:951	arg1	termini					953:959	previously overlooked glucuronic acid termini	915:959	previously overlooked glucuronic acid termini	915:959	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	2	75	theme	glycomic	481:488	arg1	analyses					509:516	glycomic and glycoproteomic analyses	481:516	glycomic and glycoproteomic analyses	481:516	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	3	76	from	subject	730:736	arg1	study					668:672	the current study	656:672	the current study	656:672	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	1	77	theme	proteins	336:343	arg1	number					326:331	a large number	318:331	a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state	318:444	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	78	theme	lepidopteran	1191:1202	arg1	species					1204:1210	lepidopteran species	1191:1210	lepidopteran species	1191:1210	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	79	theme	residues	983:990	arg1	β-mannosylation					1001:1015	core β-mannosylation	996:1015	core β-mannosylation of the N-glycans	996:1032	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	79	theme	residues	983:990	arg1	sulfation					962:970	sulfation	962:970	sulfation of mannose residues	962:990	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	4	79	theme	residues	983:990	arg1	termini					953:959	previously overlooked glucuronic acid termini	915:959	previously overlooked glucuronic acid termini	915:959	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	2	80	theme	biological	631:640	arg1	functions					642:650	specific biological functions	622:650	specific biological functions	622:650	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	5	81	theme	gland	1307:1311	arg1	cells					1313:1317	hypopharyngeal gland cells	1292:1317	hypopharyngeal gland cells	1292:1317	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	82	theme	Royal	106:110	arg1	jelly					112:116	Royal jelly	106:116	Royal jelly	106:116	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	4	83	theme	core	996:999	arg1	β-mannosylation					1001:1015	core β-mannosylation	996:1015	core β-mannosylation of the N-glycans	996:1032	Similarly to recent in-depth studies on other insect species, previously overlooked glucuronic acid termini, sulfation of mannose residues and core β-mannosylation of the N-glycans were found; additionally, a relatively rare zwitterionic modification with phosphoethanolamine is present, in contrast to the phosphorylcholine occurring in lepidopteran species.					
30104209	1	84	contain	have	381:384	arg1	proteins					336:343	proteins	336:343	proteins	336:343	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	84	contain	have	381:384	arg2	effects					405:411	various biological effects	386:411	various biological effects	386:411	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	84	contain	have	381:384	arg1	some					346:349	some	346:349	some	346:349	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	0	85	theme	Anionic	39:45	arg1	N-glycans					64:72	Anionic and Zwitterionic N-glycans	39:72	Anionic and Zwitterionic N-glycans	39:72	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	1	86	theme	hypopharyngeal	272:285	arg1	glands					287:292	the hypopharyngeal glands	268:292	the hypopharyngeal glands of worker bees	268:307	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	87	theme	benefits	226:233	arg1	claims					216:221	claims	216:221	queen honeybees as well as claims of benefits on human health	189:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	87	theme	benefits	226:233	arg1	honeybees					195:203	queen honeybees	189:203	queen honeybees as well as claims of benefits on human health	189:249	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	0	88	theme	Zwitterionic	51:62	arg1	N-glycans					64:72	Anionic and Zwitterionic N-glycans	39:72	Anionic and Zwitterionic N-glycans	39:72	Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.					
30104209	6	89	theme	multiantennary	1491:1504	arg1	structures					1506:1515	hybrid and multiantennary structures	1480:1515	structures	1506:1515	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	1	90	theme	bees	304:307	arg1	glands					287:292	the hypopharyngeal glands	268:292	the hypopharyngeal glands of worker bees	268:307	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	2	91	from	analyses	509:516	arg1	past					525:528	past	525:528	past	525:528	However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions.					
30104209	5	92	theme	fucosylated	1341:1351	arg1	glycans					1372:1378	fucosylated or paucimannosidic glycans	1341:1378	glycans	1372:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	1	93	contain	contains	309:316	arg1	product					257:263	this product	252:263	this product of the hypopharyngeal glands of worker bees	252:307	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	1	93	contain	contains	309:316	arg2	number					326:331	a large number	318:331	a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state	318:444	Royal jelly has received attention because of its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state.					
30104209	6	94	theme	hybrid	1480:1485	arg1	structures					1506:1515	hybrid and multiantennary structures	1480:1515	structures	1506:1515	The unusual modifications of hybrid and multiantennary structures defined here may not only have a physiological role in honeybee development, but represent epitopes recognized by pentraxins with roles in animal innate immunity.					
30104209	3	95	theme	current	660:666	arg1	study					668:672	the current study	656:672	the current study	656:672	In the current study, whole royal jelly as well as single protein bands were subject to off-line LC-MALDI-TOF MS glycomic analyses, complemented by permethylation, Western blotting and arraying data.					
30104209	5	96	theme	paucimannosidic	1356:1370	arg1	glycans					1372:1378	fucosylated or paucimannosidic glycans	1341:1378	glycans	1372:1378	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
30104209	5	97	theme	remodelling	1243:1253	arg1	Indicative					1213:1222	Indicative	1213:1222	Indicative	1213:1222	Indicative of tissue-specific remodelling of glycans in the Golgi apparatus of hypopharyngeal gland cells, only a low amount of fucosylated or paucimannosidic glycans were detected as compared with other insect samples or even bee venom.					
31539564	0	0	link	N-linked	64:71	arg1	glycosylation					73:85	the N-linked glycosylation	60:85	the N-linked glycosylation of IgG produced by CHO cells	60:114	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	6	1	theme	galactose	1030:1038	arg1	mannose					987:993	mannose	987:993	mannose	987:993	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	6	1	theme	galactose	1030:1038	arg1	combinations					1002:1013	the combinations	998:1013	the combinations of mannose and galactose	998:1038	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	8	2	used	used	1341:1344	arg2	lactate					1311:1317	lactate	1311:1317	lactate	1311:1317	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	8	2	used	used	1341:1344	arg2	source					1370:1375	complementary carbon source	1349:1375	complementary carbon source	1349:1375	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	9	3	used	used	1502:1505	arg2	data					1471:1474	The data	1467:1474	The data of the glycoprofiles	1467:1495	The data of the glycoprofiles were used for training the model, and then to simulate the glycosylation changes with varying the concentrations of mannose and galactose.					
31539564	2	4	theme	culture	267:273	arg1	media					275:279	cell culture media	262:279	cell culture media	262:279	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	6	5	theme	glycosylation	1112:1124	arg1	patterns					1126:1133	more matured glycosylation patterns	1099:1133	more matured glycosylation patterns	1099:1133	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	1	6	theme	glycosylation	143:155	arg1	structure					121:129	The structure	117:129	The structure of N-linked glycosylation	117:155	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	1	6	theme	glycosylation	143:155	arg1	attribute					185:193	a very important quality attribute	160:193	a very important quality attribute for therapeutic monoclonal antibodies	160:231	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	7	7	from	similar	1229:1235	arg1	conditions					1248:1257	all the conditions	1240:1257	all the conditions	1240:1257	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	1	8	theme	important	167:175	arg1	structure					121:129	The structure	117:129	The structure of N-linked glycosylation	117:155	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	1	8	theme	important	167:175	arg1	attribute					185:193	a very important quality attribute	160:193	a very important quality attribute for therapeutic monoclonal antibodies	160:231	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	9	9	theme	mannose	1613:1619	arg1	concentrations					1595:1608	the concentrations	1591:1608	the concentrations of mannose and galactose	1591:1633	The data of the glycoprofiles were used for training the model, and then to simulate the glycosylation changes with varying the concentrations of mannose and galactose.					
31539564	8	10	theme	carbon	1363:1368	arg1	lactate					1311:1317	lactate	1311:1317	lactate	1311:1317	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	8	10	theme	carbon	1363:1368	arg1	source					1370:1375	complementary carbon source	1349:1375	complementary carbon source	1349:1375	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	6	11	with	Cultivation	957:967	arg1	mannose					987:993	mannose	987:993	mannose	987:993	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	6	11	with	Cultivation	957:967	arg1	combinations					1002:1013	the combinations	998:1013	the combinations of mannose and galactose	998:1038	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	0	12	theme	CHO	106:108	arg1	cells					110:114	CHO cells	106:114	CHO cells	106:114	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	10	13	theme	predictive	1692:1701	arg1	capacity					1703:1710	a good predictive capacity	1685:1710	a good predictive capacity of the N-linked glycosylation	1685:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	9	14	theme	galactose	1625:1633	arg1	concentrations					1595:1608	the concentrations	1591:1608	the concentrations of mannose and galactose	1591:1633	The data of the glycoprofiles were used for training the model, and then to simulate the glycosylation changes with varying the concentrations of mannose and galactose.					
31539564	4	15	theme	Flux	665:668	arg1	EFM					676:678	EFM	676:678	EFM	676:678	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	4	15	theme	Flux	665:668	arg1	Mode					670:673	Elementary Flux Mode	654:673	Elementary Flux Mode (EFM)	654:679	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	5	16	with	combinations	909:920	arg1	concentrations					890:903	various concentrations	882:903	various concentrations	882:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	9	17	theme	glycoprofiles	1483:1495	arg1	data					1471:1474	The data	1467:1474	The data of the glycoprofiles	1467:1495	The data of the glycoprofiles were used for training the model, and then to simulate the glycosylation changes with varying the concentrations of mannose and galactose.					
31539564	2	18	theme	carbon	244:249	arg1	mannose					290:296	mannose	290:296	mannose	290:296	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	18	theme	carbon	244:249	arg1	galactose					302:310	galactose	302:310	galactose	302:310	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	18	theme	carbon	244:249	arg1	sources					251:257	Different carbon sources	234:257	Different carbon sources	234:257	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	8	19	theme	growth	1394:1399	arg1	rate					1401:1404	cell growth rate	1389:1404	cell growth rate	1389:1404	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	11	20	theme	processes	1823:1831	arg1	development					1783:1793	development	1783:1793	development of glycoprotein cultivation processes	1783:1831	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	0	21	theme	novel	44:48	arg1	model					50:54	A novel model	42:54	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.	0:115	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	5	22	theme	Hamster	851:857	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	10	23	theme	N-linked	1719:1726	arg1	glycosylation					1728:1740	the N-linked glycosylation	1715:1740	the N-linked glycosylation	1715:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	0	24	theme	N-linked	64:71	arg1	glycosylation					73:85	the N-linked glycosylation	60:85	the N-linked glycosylation of IgG produced by CHO cells	60:114	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	6	25	with	cultures	1151:1158	arg1	glucose					1165:1171	glucose	1165:1171	glucose	1165:1171	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	5	26	theme	glucose	925:931	arg1	combinations					909:920	combinations	909:920	combinations of glucose, mannose and galactose	909:954	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	5	26	theme	glucose	925:931	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	1	27	link	N-linked	134:141	arg1	glycosylation					143:155	N-linked glycosylation	134:155	N-linked glycosylation	134:155	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	11	28	theme	cultivation	1811:1821	arg1	processes					1823:1831	glycoprotein cultivation processes	1798:1831	glycoprotein cultivation processes	1798:1831	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	0	29	theme	Glycan	0:5	arg1	Balance					16:22	Glycan Residues Balance Analysis - GReBA	0:39	Balance	16:22	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	0	30	gly	glycosylation	73:85	arg1	IgG					90:92	IgG	90:92	IgG produced by CHO cells	90:114	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	3	31	theme	profile	445:451	arg1	control					416:422	control	416:422	control	416:422	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	3	31	theme	profile	445:451	arg1	prediction					401:410	Accurate prediction	392:410	Accurate prediction	392:410	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	3	32	theme	cultures	513:520	arg1	development					483:493	the process development	471:493	the process development of mammalian cell cultures	471:520	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	2	33	from	sources	251:257	arg1	media					275:279	cell culture media	262:279	cell culture media	262:279	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	4	34	theme	cell	745:748	arg1	cultures					750:757	steady state cell cultures	732:757	steady state cell cultures	732:757	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	11	35	theme	glycoprotein	1798:1809	arg1	processes					1823:1831	glycoprotein cultivation processes	1798:1831	glycoprotein cultivation processes	1798:1831	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	6	36	theme	cells	976:980	arg1	Cultivation					957:967	Cultivation	957:967	Cultivation of CHO cells with mannose or the combinations of mannose and galactose	957:1038	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	5	37	theme	mannose	934:940	arg1	combinations					909:920	combinations	909:920	combinations of glucose, mannose and galactose	909:954	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	5	37	theme	mannose	934:940	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	6	38	theme	decreased	1052:1060	arg1	production					1083:1092	decreased lactate and ammonium production	1052:1092	decreased lactate and ammonium production	1052:1092	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	3	39	theme	glycosylation	431:443	arg1	profile					445:451	the glycosylation profile	427:451	the glycosylation profile	427:451	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	8	40	theme	complementary	1349:1361	arg1	lactate					1311:1317	lactate	1311:1317	lactate	1311:1317	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	8	40	theme	complementary	1349:1361	arg1	source					1370:1375	complementary carbon source	1349:1375	complementary carbon source	1349:1375	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	3	41	theme	mammalian	498:506	arg1	cultures					513:520	mammalian cell cultures	498:520	mammalian cell cultures	498:520	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	5	42	theme	pseudo-perfusion	792:807	arg1	cultivation					809:819	pseudo-perfusion cultivation	792:819	pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose	792:954	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	1	43	theme	monoclonal	211:220	arg1	antibodies					222:231	therapeutic monoclonal antibodies	199:231	therapeutic monoclonal antibodies	199:231	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	5	44	theme	galactose	946:954	arg1	combinations					909:920	combinations	909:920	combinations of glucose, mannose and galactose	909:954	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	5	44	theme	galactose	946:954	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	7	45	theme	growth	1191:1196	arg1	rate					1198:1201	the growth rate	1187:1201	the growth rate	1187:1201	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	7	45	theme	growth	1191:1196	arg1	similar					1229:1235	similar	1229:1235	similar	1229:1235	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	9	46	theme	glycosylation	1556:1568	arg1	changes					1570:1576	the glycosylation changes	1552:1576	the glycosylation changes	1552:1576	The data of the glycoprofiles were used for training the model, and then to simulate the glycosylation changes with varying the concentrations of mannose and galactose.					
31539564	2	47	theme	cell	262:265	arg1	media					275:279	cell culture media	262:279	cell culture media	262:279	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	1	48	theme	N-linked	134:141	arg1	glycosylation					143:155	N-linked glycosylation	134:155	N-linked glycosylation	134:155	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	11	49	used	used	1760:1763	arg2	guidance					1770:1777	a guidance	1768:1777	a guidance for development of glycoprotein cultivation processes	1768:1831	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	11	49	used	used	1760:1763	arg2	GReBA					1747:1751	The GReBA	1743:1751	The GReBA	1743:1751	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	6	50	theme	matured	1104:1110	arg1	patterns					1126:1133	more matured glycosylation patterns	1099:1133	more matured glycosylation patterns	1099:1133	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	5	51	with	cells	871:875	arg1	concentrations					890:903	various concentrations	882:903	various concentrations	882:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	10	52	theme	GReBA	1669:1673	arg1	model					1675:1679	the GReBA model	1665:1679	the GReBA model	1665:1679	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	1	53	theme	quality	177:183	arg1	structure					121:129	The structure	117:129	The structure of N-linked glycosylation	117:155	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	1	53	theme	quality	177:183	arg1	attribute					185:193	a very important quality attribute	160:193	a very important quality attribute for therapeutic monoclonal antibodies	160:231	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	10	54	theme	good	1687:1690	arg1	capacity					1703:1710	a good predictive capacity	1685:1710	a good predictive capacity of the N-linked glycosylation	1685:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	5	55	theme	Ovary	859:863	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	11	56	gly	glycoprotein	1798:1809	arg1	glycoprotein					1798:1809	glycoprotein cultivation processes	1798:1831	glycoprotein cultivation processes	1798:1831	The GReBA can be used as a guidance for development of glycoprotein cultivation processes.					
31539564	8	57	theme	cell	1389:1392	arg1	rate					1401:1404	cell growth rate	1389:1404	cell growth rate	1389:1404	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	4	58	theme	Elementary	654:663	arg1	EFM					676:678	EFM	676:678	EFM	676:678	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	4	58	theme	Elementary	654:663	arg1	Mode					670:673	Elementary Flux Mode	654:673	Elementary Flux Mode (EFM)	654:679	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	10	59	theme	glycosylation	1728:1740	arg1	capacity					1703:1710	a good predictive capacity	1685:1710	a good predictive capacity of the N-linked glycosylation	1685:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	5	60	theme	CHO	866:868	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	6	61	theme	CHO	972:974	arg1	cells					976:980	CHO cells	972:980	CHO cells	972:980	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	4	62	theme	Mode	670:673	arg1	concept					643:649	the concept	639:649	the concept of Elementary Flux Mode (EFM)	639:679	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	7	63	theme	IgG	1207:1209	arg1	productivity					1211:1222	IgG productivity	1207:1222	IgG productivity	1207:1222	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	2	64	theme	Different	234:242	arg1	mannose					290:296	mannose	290:296	mannose	290:296	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	64	theme	Different	234:242	arg1	galactose					302:310	galactose	302:310	galactose	302:310	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	64	theme	Different	234:242	arg1	sources					251:257	Different carbon sources	234:257	Different carbon sources	234:257	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	5	65	theme	antibody	824:831	arg1	cultivation					809:819	pseudo-perfusion cultivation	792:819	pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose	792:954	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	8	66	theme	IgG	1410:1412	arg1	productivity					1414:1425	IgG productivity	1410:1425	IgG productivity	1410:1425	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	5	67	theme	Chinese	843:849	arg1	cells					871:875	Chinese Hamster Ovary (CHO) cells	843:875	Chinese Hamster Ovary (CHO) cells with various concentrations	843:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	8	68	theme	comparable	1427:1436	arg1	rate					1401:1404	cell growth rate	1389:1404	cell growth rate	1389:1404	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	0	69	dep	Balance	16:22	arg1	Analysis					24:31	Analysis	24:31	Analysis	24:31	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	0	69	dep	Balance	16:22	arg1	model					50:54	A novel model	42:54	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.	0:115	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	10	70	link	N-linked	1719:1726	arg1	glycosylation					1728:1740	the N-linked glycosylation	1715:1740	the N-linked glycosylation	1715:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	0	71	theme	IgG	90:92	arg1	glycosylation					73:85	the N-linked glycosylation	60:85	the N-linked glycosylation of IgG produced by CHO cells	60:114	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	6	72	theme	mannose	1018:1024	arg1	mannose					987:993	mannose	987:993	mannose	987:993	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	6	72	theme	mannose	1018:1024	arg1	combinations					1002:1013	the combinations	998:1013	the combinations of mannose and galactose	998:1038	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	10	73	contain	had	1681:1683	arg2	capacity					1703:1710	a good predictive capacity	1685:1710	a good predictive capacity of the N-linked glycosylation	1685:1740	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	10	73	contain	had	1681:1683	arg1	model					1675:1679	the GReBA model	1665:1679	the GReBA model	1665:1679	In this study we showed that the GReBA model had a good predictive capacity of the N-linked glycosylation.					
31539564	8	74	theme	other	1453:1457	arg1	sugars					1459:1464	the other sugars	1449:1464	the other sugars	1449:1464	When the cells were cultured with galactose alone, lactate was fed as well to be used as complementary carbon source, leading to cell growth rate and IgG productivity comparable to feeding the other sugars.					
31539564	0	75	theme	Residues	7:14	arg1	Balance					16:22	Glycan Residues Balance Analysis - GReBA	0:39	Balance	16:22	Glycan Residues Balance Analysis - GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells.					
31539564	2	76	contain	have	335:338	arg1	mannose					290:296	mannose	290:296	mannose	290:296	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	76	contain	have	335:338	arg1	galactose					302:310	galactose	302:310	galactose	302:310	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	76	contain	have	335:338	arg2	influences					350:359	different influences	340:359	different influences	340:359	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	76	contain	have	335:338	arg1	sources					251:257	Different carbon sources	234:257	Different carbon sources	234:257	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	2	77	theme	different	340:348	arg1	influences					350:359	different influences	340:359	different influences	340:359	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	4	78	theme	steady	732:737	arg1	cultures					750:757	steady state cell cultures	732:757	steady state cell cultures	732:757	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	5	79	theme	various	882:888	arg1	concentrations					890:903	various concentrations	882:903	various concentrations	882:903	Experiments were carried out in pseudo-perfusion cultivation of antibody producing Chinese Hamster Ovary (CHO) cells with various concentrations and combinations of glucose, mannose and galactose.					
31539564	4	80	theme	mathematical	540:551	arg1	model					553:557	a mathematical model	538:557	a mathematical model	538:557	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	3	81	theme	Accurate	392:399	arg1	prediction					401:410	Accurate prediction	392:410	Accurate prediction	392:410	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	4	82	theme	state	739:743	arg1	cultures					750:757	steady state cell cultures	732:757	steady state cell cultures	732:757	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	2	83	theme	glycosylation	368:380	arg1	patterns					382:389	the glycosylation patterns	364:389	the glycosylation patterns	364:389	Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on the glycosylation patterns.					
31539564	6	84	theme	ammonium	1074:1081	arg1	production					1083:1092	decreased lactate and ammonium production	1052:1092	decreased lactate and ammonium production	1052:1092	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	6	85	dep	resulted	1040:1047	arg1	compared					1135:1142	compared	1135:1142	compared to the cultures with glucose	1135:1171	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	3	86	theme	process	475:481	arg1	development					483:493	the process development	471:493	the process development of mammalian cell cultures	471:520	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31539564	7	87	from	conditions	1248:1257	arg1	rate					1198:1201	the growth rate	1187:1201	the growth rate	1187:1201	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	7	87	from	conditions	1248:1257	arg1	similar					1229:1235	similar	1229:1235	similar	1229:1235	Furthermore, the growth rate and IgG productivity were similar in all the conditions.					
31539564	6	88	theme	lactate	1062:1068	arg1	production					1083:1092	decreased lactate and ammonium production	1052:1092	decreased lactate and ammonium production	1052:1092	Cultivation of CHO cells with mannose or the combinations of mannose and galactose resulted in decreased lactate and ammonium production, and more matured glycosylation patterns compared to the cultures with glucose.					
31539564	4	89	theme	Glycan	574:579	arg1	Residues					581:588	Glycan Residues	574:588	Glycan Residues	574:588	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	4	90	theme	glycosylation	706:718	arg1	profile					720:726	the glycosylation profile	702:726	the glycosylation profile for steady state cell cultures	702:757	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	4	91	used	used	686:689	arg2	model					553:557	a mathematical model	538:557	a mathematical model	538:557	In this study, a mathematical model, that we named Glycan Residues Balance Analysis (GReBA), was developed based on the concept of Elementary Flux Mode (EFM), and used to predict the glycosylation profile for steady state cell cultures.					
31539564	1	92	theme	therapeutic	199:209	arg1	antibodies					222:231	therapeutic monoclonal antibodies	199:231	therapeutic monoclonal antibodies	199:231	The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies.					
31539564	3	93	theme	cell	508:511	arg1	cultures					513:520	mammalian cell cultures	498:520	mammalian cell cultures	498:520	Accurate prediction and control of the glycosylation profile are important for the process development of mammalian cell cultures.					
31256372	4	0	theme	prior	570:574	arg1	detergents					559:568	detergents	559:568	detergents prior to PNGase F digestion	559:596	The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion.					
31256372	5	1	dep	2AB	783:785	arg1	label					777:781	label	777:781	label	777:781	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	4	2	theme	protein	503:509	arg1	structure					511:519	protein structure	503:519	protein structure	503:519	The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion.					
31256372	5	3	attach	isolated	625:632	arg1	peptides					639:646	peptides	639:646	peptides	639:646	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	5	3	attach	isolated	625:632	arg2	N-glycans					611:619	N-glycans	611:619	N-glycans	611:619	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	4	4	theme	detergents	559:568	arg1	use					552:554	the use	548:554	the use of detergents prior to PNGase F digestion	548:596	The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion.					
31256372	2	5	theme	chemical	287:294	arg1	release					303:309	chemical glycan release	287:309	chemical glycan release	287:309	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	2	6	theme	glycan	296:301	arg1	release					303:309	chemical glycan release	287:309	chemical glycan release	287:309	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	1	7	theme	diseases	262:269	arg1	course					252:257	the course	248:257	the course of diseases	248:269	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	5	8	theme	reverse-phase	654:666	arg1	cartridges					668:677	reverse-phase cartridges	654:677	reverse-phase cartridges	654:677	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	2	9	dep	release	303:309	arg1	contrary					275:282	contrary	275:282	contrary	275:282	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	2	10	theme	relative	384:391	arg1	ease					393:396	relative ease	384:396	relative ease	384:396	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	2	11	theme	enzymatic	312:320	arg1	cleavage					322:329	enzymatic cleavage	312:329	enzymatic cleavage of N-glycans	312:342	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	1	12	theme	cell-cell	184:192	arg1	interactions					194:205	cell-cell interactions	184:205	cell-cell interactions	184:205	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	0	13	theme	Enzymatic	0:8	arg1	Release					10:16	Enzymatic Release	0:16	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling	0:67	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.					
31256372	1	14	theme	interactions	194:205	arg1	mechanisms					170:179	the mechanisms	166:179	the mechanisms of cell-cell interactions and immune reactions	166:226	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	0	15	theme	Glycoprotein	21:32	arg1	N-Glycans					34:42	Glycoprotein N-Glycans	21:42	Glycoprotein N-Glycans	21:42	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.					
31256372	5	16	theme	carbograph	701:710	arg1	cartridges					712:721	carbograph cartridges	701:721	carbograph cartridges	701:721	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	1	17	theme	frequent	101:108	arg1	modifications					136:148	the most frequent protein posttranslational modifications	92:148	the most frequent protein posttranslational modifications	92:148	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	1	18	theme	immune	211:216	arg1	reactions					218:226	immune reactions	211:226	immune reactions	211:226	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	2	19	theme	N-glycans	334:342	arg1	cleavage					322:329	enzymatic cleavage	312:329	enzymatic cleavage of N-glycans	312:342	In contrary to chemical glycan release, enzymatic cleavage of N-glycans can be performed in any laboratory with relative ease.					
31256372	3	20	theme	robust	419:424	arg1	protocols					426:434	two robust protocols	415:434	two robust protocols	415:434	We present here two robust protocols to achieve N-glycan release.					
31256372	1	21	theme	reactions	218:226	arg1	mechanisms					170:179	the mechanisms	166:179	the mechanisms of cell-cell interactions and immune reactions	166:226	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	0	22	theme	N-Glycans	34:42	arg1	Release					10:16	Enzymatic Release	0:16	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling	0:67	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.					
31256372	3	23	theme	N-glycan	447:454	arg1	release					456:462	N-glycan release	447:462	N-glycan release	447:462	We present here two robust protocols to achieve N-glycan release.					
31256372	0	24	theme	Labeling	60:67	arg1	Release					10:16	Enzymatic Release	0:16	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling	0:67	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.					
31256372	5	25	theme	fluorescent	765:775	arg1	2AB					783:785	the fluorescent label 2AB	761:785	the fluorescent label 2AB	761:785	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	1	26	theme	protein	110:116	arg1	modifications					136:148	the most frequent protein posttranslational modifications	92:148	the most frequent protein posttranslational modifications	92:148	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	0	27	theme	Fluorescent	48:58	arg1	Labeling					60:67	Fluorescent Labeling	48:67	Fluorescent Labeling	48:67	Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.					
31256372	1	28	theme	posttranslational	118:134	arg1	modifications					136:148	the most frequent protein posttranslational modifications	92:148	the most frequent protein posttranslational modifications	92:148	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	1	29	theme	modifications	136:148	arg1	modifications					136:148	the most frequent protein posttranslational modifications	92:148	the most frequent protein posttranslational modifications	92:148	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	1	29	theme	modifications	136:148	arg1	one					85:87	one	85:87	one	85:87	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	1	29	theme	modifications	136:148	arg1	Glycosylation					70:82	Glycosylation	70:82	Glycosylation	70:82	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	4	30	theme	F	586:586	arg1	digestion					588:596	PNGase F digestion	579:596	PNGase F digestion	579:596	The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion.					
31256372	1	31	gly	Glycosylation	70:82	arg1	mechanisms					170:179	the mechanisms	166:179	the mechanisms of cell-cell interactions and immune reactions	166:226	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	1	31	gly	Glycosylation	70:82	arg1	course					252:257	the course	248:257	the course of diseases	248:269	Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune reactions and is modulated in the course of diseases.					
31256372	5	32	with	desalted	687:694	arg1	cartridges					712:721	carbograph cartridges	701:721	carbograph cartridges	701:721	Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.					
31256372	4	33	theme	PNGase	579:584	arg1	digestion					588:596	PNGase F digestion	579:596	PNGase F digestion	579:596	The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion.					
29411222	6	0	theme	Fc	930:931	arg1	originator					943:952	originator	943:952	originator	943:952	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	0	theme	Fc	930:931	arg1	product					968:974	follow-on product	958:974	follow-on product	958:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	0	theme	Fc	930:931	arg1	domain					933:938	the Fc domain	926:938	the Fc domain of originator and follow-on product	926:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	10	1	theme	prior	1574:1578	arg1	cleavage					1580:1587	prior cleavage	1574:1587	prior cleavage of the sialic acid residues for the first time	1574:1634	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	11	2	theme	mobility	1654:1661	arg1	data					1676:1679	ion mobility spectrometry data	1650:1679	ion mobility spectrometry data	1650:1679	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	10	3	theme	residues	1608:1615	arg1	cleavage					1580:1587	prior cleavage	1574:1587	prior cleavage of the sialic acid residues for the first time	1574:1634	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	9	4	theme	1-type	1467:1472	arg1	glycans					1474:1480	core 1-type glycans	1462:1480	core 1-type glycans	1462:1480	Four different O-glycopeptides bearing core 1-type glycans were detected.					
29411222	6	5	theme	complete	905:912	arg1	monomer					914:920	the complete monomer	901:920	the complete monomer	901:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	10	6	theme	sialic	1596:1601	arg1	residues					1608:1615	the sialic acid residues	1592:1615	the sialic acid residues for the first time	1592:1634	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	11	7	theme	close	1691:1695	arg1	similarity					1697:1706	close similarity	1691:1706	close similarity of higher-order structure of both biologics	1691:1750	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	1	8	theme	receptor	187:194	arg1	domain					217:222	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	12	9	theme	neutralization	1768:1781	arg1	assay					1783:1787	a neutralization assay	1766:1787	a neutralization assay	1766:1787	Furthermore, a neutralization assay, investigating the impact of altered PTMs on potency, indicated that the differences within all batches are still in the acceptable range for biosimilarity.					
29411222	4	10	theme	biosimilar	673:682	arg1	Altebrel™					684:692	its biosimilar Altebrel™	669:692	its biosimilar Altebrel™ (AryoGen Pharmed)	669:710	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	10	theme	biosimilar	673:682	arg1	Pharmed					703:709	Pharmed	703:709	Pharmed	703:709	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	10	theme	biosimilar	673:682	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	2	11	theme	O-glycosylation	284:298	arg1	sites					300:304	three N- and 13 O-glycosylation sites	268:304	sites	300:304	It possesses three N- and 13 O-glycosylation sites.					
29411222	7	12	gly	sialylated	1236:1245	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	1	13	theme	extracellular	203:215	arg1	domain					217:222	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	6	14	theme	product	968:974	arg1	domain					933:938	the Fc domain	926:938	the Fc domain of originator and follow-on product	926:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	14	theme	product	968:974	arg1	originator					943:952	originator	943:952	originator	943:952	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	14	theme	product	968:974	arg1	product					968:974	follow-on product	958:974	follow-on product	958:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	14	theme	product	968:974	arg1	species					889:895	Similar glycoprotein species	868:895	Similar glycoprotein species for the complete monomer	868:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	7	15	from	N317	1205:1208	arg1	N171					1267:1270	N171	1267:1270	N171	1267:1270	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	15	from	N317	1205:1208	arg1	N149					1258:1261	N149	1258:1261	N149	1258:1261	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	15	from	N317	1205:1208	arg1	products					1293:1300	both investigated products	1275:1300	both investigated products	1275:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	10	16	gly	O-glycopeptide	1514:1527	arg2	O-glycopeptide					1514:1527	O-glycopeptide analysis	1514:1536	O-glycopeptide analysis	1514:1536	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	4	17	theme	originator	646:655	arg1	Enbrel®					657:663	Etanercept originator Enbrel®	635:663	Etanercept originator Enbrel®	635:663	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	1	18	attach	linked	225:230	arg2	Etanercept					119:128	Etanercept	119:128	Etanercept	119:128	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	18	attach	linked	225:230	arg2	protein					150:156	a soluble fusion protein	133:156	a soluble fusion protein	133:156	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	18	attach	linked	225:230	arg1	part					241:244	an Fc part	235:244	an Fc part of IgG1	235:252	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	8	19	theme	Small	1303:1307	arg1	variations					1322:1331	Small quantitative variations	1303:1331	Small quantitative variations in the N-glycan profile	1303:1355	Small quantitative variations in the N-glycan profile were detected, although the N-glycans were qualitatively similar.					
29411222	7	20	dep	sites	1137:1141	arg1	sites					1137:1141	the N-glycosylation sites	1117:1141	the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1117:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	20	dep	sites	1137:1141	arg1	N317					1159:1162	N317	1159:1162	N317	1159:1162	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	20	dep	sites	1137:1141	arg1	N171					1150:1153	N171	1150:1153	N171	1150:1153	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	1	21	theme	soluble	135:141	arg1	protein					150:156	a soluble fusion protein	133:156	a soluble fusion protein	133:156	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	21	theme	soluble	135:141	arg1	Etanercept					119:128	Etanercept	119:128	Etanercept	119:128	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	7	22	from	glycans	1247:1253	arg1	N171					1267:1270	N171	1267:1270	N171	1267:1270	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	22	from	glycans	1247:1253	arg1	N149					1258:1261	N149	1258:1261	N149	1258:1261	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	22	from	glycans	1247:1253	arg1	products					1293:1300	both investigated products	1275:1300	both investigated products	1275:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	1	23	theme	IgG1	249:252	arg1	part					241:244	an Fc part	235:244	an Fc part of IgG1	235:252	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	0	24	theme	Fusion	92:97	arg1	Etanercept					107:116	the Fusion Protein Etanercept	88:116	the Fusion Protein Etanercept	88:116	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	8	25	from	variations	1322:1331	arg1	profile					1349:1355	the N-glycan profile	1336:1355	the N-glycan profile	1336:1355	Small quantitative variations in the N-glycan profile were detected, although the N-glycans were qualitatively similar.					
29411222	1	26	theme	necrosis	171:178	arg1	TNFR					197:200	TNFR	197:200	TNFR	197:200	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	26	theme	necrosis	171:178	arg1	receptor					187:194	tumor necrosis factor receptor	165:194	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	6	27	theme	small	1000:1004	arg1	differences					1006:1016	small differences	1000:1016	small differences in lysine variants and oxidation	1000:1049	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	7	28	theme	TNFR-specific	1215:1227	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	0	29	theme	Etanercept	107:116	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization	0:31	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	0	29	theme	Etanercept	107:116	arg1	Pattern					48:54	Glycosylation Pattern	34:54	Glycosylation Pattern	34:54	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	0	29	theme	Etanercept	107:116	arg1	Assessment					74:83	Biosimilarity Assessment	60:83	Biosimilarity Assessment	60:83	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	3	30	theme	complex	318:324	arg1	structure					326:334	its complex structure	314:334	its complex structure	314:334	Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars.					
29411222	4	31	theme	physicochemical	438:452	arg1	characterization					454:469	physicochemical characterization	438:469	physicochemical characterization using state-of-the-art analytics	438:502	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	7	32	from	glycosylation	1188:1200	arg1	N317					1205:1208	N317	1205:1208	N317	1205:1208	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	32	from	glycosylation	1188:1200	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	6	33	from	differences	1006:1016	arg1	oxidation					1041:1049	oxidation	1041:1049	oxidation	1041:1049	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	33	from	differences	1006:1016	arg1	variants					1028:1035	lysine variants	1021:1035	lysine variants	1021:1035	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	4	34	theme	quality	738:744	arg1	attributes					746:755	critical quality attributes	729:755	critical quality attributes of biopharmaceuticals	729:777	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	0	35	theme	Physicochemical	0:14	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization	0:31	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	3	36	theme	analytical	340:349	arg1	challenge					351:359	an analytical challenge	337:359	an analytical challenge	337:359	Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars.					
29411222	7	37	with	analysis	1078:1085	arg1	UHPLC-QTOF-MSE					1092:1105	UHPLC-QTOF-MSE	1092:1105	UHPLC-QTOF-MSE	1092:1105	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	4	38	theme	post-translational	556:573	arg1	PTMs					590:593	PTMs	590:593	PTMs	590:593	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	38	theme	post-translational	556:573	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	38	theme	post-translational	556:573	arg1	modifications					575:587	post-translational modifications	556:587	post-translational modifications (PTMs)	556:594	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	10	39	theme	O-glycopeptide	1514:1527	arg1	analysis					1529:1536	O-glycopeptide analysis	1514:1536	O-glycopeptide analysis	1514:1536	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	11	40	theme	biologics	1742:1750	arg1	structure					1724:1732	higher-order structure	1711:1732	higher-order structure of both biologics	1711:1750	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	5	41	theme	subunit	796:802	arg1	analysis					804:811	Intact mass and subunit analysis	780:811	analysis	804:811	Intact mass and subunit analysis revealed a size of about 126 kDa for both biologicals.					
29411222	6	42	theme	Similar	868:874	arg1	species					889:895	Similar glycoprotein species	868:895	Similar glycoprotein species for the complete monomer	868:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	0	43	theme	Biosimilarity	60:72	arg1	Assessment					74:83	Biosimilarity Assessment	60:83	Biosimilarity Assessment	60:83	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	7	44	gly	glycosylation	1188:1200	arg1	N317					1205:1208	N317	1205:1208	N317	1205:1208	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	44	gly	glycosylation	1188:1200	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	45	from	sites	1137:1141	arg1	N317					1205:1208	N317	1205:1208	N317	1205:1208	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	45	from	sites	1137:1141	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	4	46	theme	higher	597:602	arg1	structure					610:618	higher order structure	597:618	higher order structure	597:618	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	46	theme	higher	597:602	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	2	47	gly	O-glycosylation	284:298	arg2	sites					300:304	three N- and 13 O-glycosylation sites	268:304	sites	300:304	It possesses three N- and 13 O-glycosylation sites.					
29411222	2	47	gly	O-glycosylation	284:298	arg2	13					281:282	13	281:282	13	281:282	It possesses three N- and 13 O-glycosylation sites.					
29411222	11	48	theme	ion	1650:1652	arg1	data					1676:1679	ion mobility spectrometry data	1650:1679	ion mobility spectrometry data	1650:1679	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	4	49	theme	Enbrel®	657:663	arg1	potency					624:630	potency	624:630	potency	624:630	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	49	theme	Enbrel®	657:663	arg1	Pharmed					703:709	Pharmed	703:709	Pharmed	703:709	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	49	theme	Enbrel®	657:663	arg1	structure					610:618	higher order structure	597:618	higher order structure	597:618	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	49	theme	Enbrel®	657:663	arg1	Altebrel™					684:692	its biosimilar Altebrel™	669:692	its biosimilar Altebrel™ (AryoGen Pharmed)	669:710	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	49	theme	Enbrel®	657:663	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	5	50	theme	Intact	780:785	arg1	mass					787:790	Intact mass and subunit analysis	780:811	mass	787:790	Intact mass and subunit analysis revealed a size of about 126 kDa for both biologicals.					
29411222	11	51	theme	spectrometry	1663:1674	arg1	data					1676:1679	ion mobility spectrometry data	1650:1679	ion mobility spectrometry data	1650:1679	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	9	52	theme	core	1462:1465	arg1	glycans					1474:1480	core 1-type glycans	1462:1480	core 1-type glycans	1462:1480	Four different O-glycopeptides bearing core 1-type glycans were detected.					
29411222	12	53	theme	altered	1818:1824	arg1	PTMs					1826:1829	altered PTMs	1818:1829	altered PTMs	1818:1829	Furthermore, a neutralization assay, investigating the impact of altered PTMs on potency, indicated that the differences within all batches are still in the acceptable range for biosimilarity.					
29411222	10	54	theme	acid	1603:1606	arg1	residues					1608:1615	the sialic acid residues	1592:1615	the sialic acid residues for the first time	1592:1634	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	11	55	theme	higher-order	1711:1722	arg1	structure					1724:1732	higher-order structure	1711:1732	higher-order structure of both biologics	1711:1750	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	7	56	theme	N-glycosylation	1121:1135	arg1	sites					1137:1141	the N-glycosylation sites	1117:1141	the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1117:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	56	theme	N-glycosylation	1121:1135	arg1	N317					1159:1162	N317	1159:1162	N317	1159:1162	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	56	theme	N-glycosylation	1121:1135	arg1	N171					1150:1153	N171	1150:1153	N171	1150:1153	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	1	57	theme	factor	180:185	arg1	TNFR					197:200	TNFR	197:200	TNFR	197:200	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	57	theme	factor	180:185	arg1	receptor					187:194	tumor necrosis factor receptor	165:194	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	7	58	gly	N-glycosylation	1121:1135	arg2	N171					1150:1153	N171	1150:1153	N171	1150:1153	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	58	gly	N-glycosylation	1121:1135	arg2	sites					1137:1141	the N-glycosylation sites	1117:1141	the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1117:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	7	58	gly	N-glycosylation	1121:1135	arg2	N317					1159:1162	N317	1159:1162	N317	1159:1162	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	10	59	theme	first	1625:1629	arg1	time					1631:1634	the first time	1621:1634	the first time	1621:1634	For both, N- and O-glycopeptide analysis, determination was achieved without prior cleavage of the sialic acid residues for the first time.					
29411222	5	60	theme	126 kDa	838:844	arg1	size					824:827	a size	822:827	a size of about 126 kDa for both biologicals	822:865	Intact mass and subunit analysis revealed a size of about 126 kDa for both biologicals.					
29411222	4	61	theme	current	423:429	arg1	study					431:435	the current study	419:435	the current study	419:435	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	7	62	theme	N-Glycopeptide	1063:1076	arg1	analysis					1078:1085	N-Glycopeptide analysis	1063:1085	N-Glycopeptide analysis with UHPLC-QTOF-MSE	1063:1105	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	1	63	theme	domain	217:222	arg1	protein					150:156	a soluble fusion protein	133:156	a soluble fusion protein	133:156	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	63	theme	domain	217:222	arg1	Etanercept					119:128	Etanercept	119:128	Etanercept	119:128	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	6	64	theme	follow-on	958:966	arg1	product					968:974	follow-on product	958:974	follow-on product	958:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	4	65	theme	Etanercept	635:644	arg1	Enbrel®					657:663	Etanercept originator Enbrel®	635:663	Etanercept originator Enbrel®	635:663	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	6	66	theme	originator	943:952	arg1	domain					933:938	the Fc domain	926:938	the Fc domain of originator and follow-on product	926:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	66	theme	originator	943:952	arg1	originator					943:952	originator	943:952	originator	943:952	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	66	theme	originator	943:952	arg1	product					968:974	follow-on product	958:974	follow-on product	958:974	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	6	66	theme	originator	943:952	arg1	species					889:895	Similar glycoprotein species	868:895	Similar glycoprotein species for the complete monomer	868:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	1	67	theme	fusion	143:148	arg1	protein					150:156	a soluble fusion protein	133:156	a soluble fusion protein	133:156	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	67	theme	fusion	143:148	arg1	Etanercept					119:128	Etanercept	119:128	Etanercept	119:128	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	8	68	theme	quantitative	1309:1320	arg1	variations					1322:1331	Small quantitative variations	1303:1331	Small quantitative variations in the N-glycan profile	1303:1355	Small quantitative variations in the N-glycan profile were detected, although the N-glycans were qualitatively similar.					
29411222	7	69	theme	sialylated	1236:1245	arg1	glycans					1247:1253	TNFR-specific highly sialylated glycans	1215:1253	TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1215:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	6	70	theme	lysine	1021:1026	arg1	variants					1028:1035	lysine variants	1021:1035	lysine variants	1021:1035	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	0	71	theme	Protein	99:105	arg1	Etanercept					107:116	the Fusion Protein Etanercept	88:116	the Fusion Protein Etanercept	88:116	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	1	72	theme	tumor	165:169	arg1	TNFR					197:200	TNFR	197:200	TNFR	197:200	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	1	72	theme	tumor	165:169	arg1	receptor					187:194	tumor necrosis factor receptor	165:194	the tumor necrosis factor receptor (TNFR) extracellular domain	161:222	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	8	73	theme	N-glycan	1340:1347	arg1	profile					1349:1355	the N-glycan profile	1336:1355	the N-glycan profile	1336:1355	Small quantitative variations in the N-glycan profile were detected, although the N-glycans were qualitatively similar.					
29411222	4	74	theme	biopharmaceuticals	760:777	arg1	attributes					746:755	critical quality attributes	729:755	critical quality attributes of biopharmaceuticals	729:777	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	75	theme	state-of-the-art	477:492	arg1	analytics					494:502	state-of-the-art analytics	477:502	state-of-the-art analytics	477:502	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	3	76	dep	development	375:385	arg1	the					371:373	the	371:373	the	371:373	Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars.					
29411222	7	77	theme	Fc-specific	1176:1186	arg1	glycosylation					1188:1200	Fc-specific glycosylation	1176:1200	the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products	1117:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	12	78	from	impact	1808:1813	arg1	potency					1834:1840	potency	1834:1840	potency	1834:1840	Furthermore, a neutralization assay, investigating the impact of altered PTMs on potency, indicated that the differences within all batches are still in the acceptable range for biosimilarity.					
29411222	4	79	theme	critical	729:736	arg1	attributes					746:755	critical quality attributes	729:755	critical quality attributes of biopharmaceuticals	729:777	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	6	80	gly	glycoprotein	876:887	arg1	glycoprotein					876:887	Similar glycoprotein species	868:895	Similar glycoprotein species for the complete monomer	868:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	3	81	theme	biosimilars	403:413	arg1	development					375:385	development	375:385	development	375:385	Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars.					
29411222	3	81	theme	biosimilars	403:413	arg1	approval					391:398	approval	391:398	approval	391:398	Due to its complex structure, an analytical challenge is facing the development and approval of biosimilars.					
29411222	0	82	theme	Glycosylation	34:46	arg1	Pattern					48:54	Glycosylation Pattern	34:54	Glycosylation Pattern	34:54	Physicochemical Characterization, Glycosylation Pattern and Biosimilarity Assessment of the Fusion Protein Etanercept.					
29411222	2	83	contain	possesses	258:266	arg2	sites					300:304	three N- and 13 O-glycosylation sites	268:304	sites	300:304	It possesses three N- and 13 O-glycosylation sites.					
29411222	2	83	contain	possesses	258:266	arg1	It					255:256	It	255:256	It	255:256	It possesses three N- and 13 O-glycosylation sites.					
29411222	2	83	contain	possesses	258:266	arg2	N-					274:275	three N- and 13 O-glycosylation sites	268:304	N-	274:275	It possesses three N- and 13 O-glycosylation sites.					
29411222	12	84	theme	acceptable	1910:1919	arg1	range					1921:1925	the acceptable range	1906:1925	the acceptable range for biosimilarity	1906:1943	Furthermore, a neutralization assay, investigating the impact of altered PTMs on potency, indicated that the differences within all batches are still in the acceptable range for biosimilarity.					
29411222	6	85	theme	glycoprotein	876:887	arg1	species					889:895	Similar glycoprotein species	868:895	Similar glycoprotein species for the complete monomer	868:920	Similar glycoprotein species for the complete monomer and the Fc domain of originator and follow-on product were observed, however, small differences in lysine variants and oxidation were found.					
29411222	4	86	theme	intact	529:534	arg1	Altebrel™					684:692	its biosimilar Altebrel™	669:692	its biosimilar Altebrel™ (AryoGen Pharmed)	669:710	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	86	theme	intact	529:534	arg1	structure					610:618	higher order structure	597:618	higher order structure	597:618	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	86	theme	intact	529:534	arg1	potency					624:630	potency	624:630	potency	624:630	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	86	theme	intact	529:534	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	86	theme	intact	529:534	arg1	modifications					575:587	post-translational modifications	556:587	post-translational modifications (PTMs)	556:594	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	11	87	theme	structure	1724:1732	arg1	similarity					1697:1706	close similarity	1691:1706	close similarity of higher-order structure of both biologics	1691:1750	In addition, ion mobility spectrometry data confirmed close similarity of higher-order structure of both biologics.					
29411222	7	88	theme	investigated	1280:1291	arg1	products					1293:1300	both investigated products	1275:1300	both investigated products	1275:1300	N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed the N-glycosylation sites (N149, N171 and N317) as well as Fc-specific glycosylation on N317, and TNFR-specific highly sialylated glycans on N149 and N171 on both investigated products.					
29411222	4	89	theme	subunit	540:546	arg1	Altebrel™					684:692	its biosimilar Altebrel™	669:692	its biosimilar Altebrel™ (AryoGen Pharmed)	669:710	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	89	theme	subunit	540:546	arg1	structure					610:618	higher order structure	597:618	higher order structure	597:618	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	89	theme	subunit	540:546	arg1	potency					624:630	potency	624:630	potency	624:630	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	89	theme	subunit	540:546	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	89	theme	subunit	540:546	arg1	modifications					575:587	post-translational modifications	556:587	post-translational modifications (PTMs)	556:594	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	90	theme	order	604:608	arg1	structure					610:618	higher order structure	597:618	higher order structure	597:618	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	4	90	theme	order	604:608	arg1	masses					548:553	intact and subunit masses	529:553	masses	548:553	In the current study, physicochemical characterization using state-of-the-art analytics was performed to analyze intact and subunit masses, post-translational modifications (PTMs), higher order structure and potency of Etanercept originator Enbrel® and its biosimilar Altebrel™ (AryoGen Pharmed) in accordance to critical quality attributes of biopharmaceuticals.					
29411222	9	91	gly	O-glycopeptides	1438:1452	arg2	O-glycopeptides					1438:1452	Four different O-glycopeptides	1423:1452	Four different O-glycopeptides bearing core 1-type glycans	1423:1480	Four different O-glycopeptides bearing core 1-type glycans were detected.					
29411222	12	92	theme	PTMs	1826:1829	arg1	impact					1808:1813	the impact	1804:1813	the impact of altered PTMs on potency	1804:1840	Furthermore, a neutralization assay, investigating the impact of altered PTMs on potency, indicated that the differences within all batches are still in the acceptable range for biosimilarity.					
29411222	1	93	theme	Fc	238:239	arg1	part					241:244	an Fc part	235:244	an Fc part of IgG1	235:252	Etanercept is a soluble fusion protein of the tumor necrosis factor receptor (TNFR) extracellular domain, linked to an Fc part of IgG1.					
29411222	9	94	theme	different	1428:1436	arg1	O-glycopeptides					1438:1452	Four different O-glycopeptides	1423:1452	Four different O-glycopeptides bearing core 1-type glycans	1423:1480	Four different O-glycopeptides bearing core 1-type glycans were detected.					
31098956	4	0	theme	engine	909:914	arg1	GlySeeker					916:924	engine GlySeeker	909:924	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	2	1	theme	confidence	616:625	arg1	interpretation					575:588	best interpretation	570:588	best interpretation of MS/MS data and highest confidence of identification	570:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	2	theme	brain	1335:1339	arg1	study					1320:1324	future glycosylation study	1299:1324	future glycosylation study of mouse brain	1299:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	2	3	theme	mass	353:356	arg1	spectrometry					358:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	2	4	theme	identification	630:643	arg1	data					599:602	MS/MS data	593:602	MS/MS data	593:602	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	2	4	theme	identification	630:643	arg1	confidence					616:625	highest confidence	608:625	highest confidence of identification	608:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	1	5	theme	N-glycans	263:271	arg1	structure					177:185	the topological structure	161:185	the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans	161:271	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	5	theme	N-glycans	263:271	arg1	information					282:292	vital information	276:292	vital information to understand their biological functions and roles	276:343	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	4	6	theme	developed	874:882	arg1	search					902:907	our recently developed N-glycan database search	861:907	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	6	7	theme	glycosylation	1306:1318	arg1	study					1320:1324	future glycosylation study	1299:1324	future glycosylation study of mouse brain	1299:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	8	from	mode	1172:1175	arg1	ions					1124:1127	product ions	1116:1127	product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1116:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	1	9	theme	topological	165:175	arg1	structure					177:185	the topological structure	161:185	the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans	161:271	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	9	theme	topological	165:175	arg1	information					282:292	vital information	276:292	vital information to understand their biological functions and roles	276:343	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	4	10	theme	database	893:900	arg1	search					902:907	our recently developed N-glycan database search	861:907	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	2	11	theme	highest	608:614	arg1	confidence					616:625	highest confidence	608:625	highest confidence of identification	608:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	12	theme	negative	1547:1554	arg1	mode					1556:1559	the negative mode	1543:1559	the negative mode	1543:1559	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	4	13	theme	topological	962:972	arg1	structures					974:983	putative topological structures	953:983	putative topological structures	953:983	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	3	14	theme	N-glycans	769:777	arg1	study					675:679	our glycomic study	662:679	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	3	14	theme	N-glycans	769:777	arg1	analysis					742:749	fragmentation pathway analysis	720:749	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	6	15	theme	tandem	1376:1381	arg1	spectra					1388:1394	tandem mass spectra	1376:1394	tandem mass spectra of N-glycans	1376:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	16	theme	ions	1124:1127	arg1	characteristics					1097:1111	characteristics	1097:1111	characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1097:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	16	theme	ions	1124:1127	arg1	Analysis					1058:1065	Analysis	1058:1065	Analysis of fragmentation pathways	1058:1091	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	0	17	from	Brain	87:91	arg1	Pathways					45:52	Pathways	45:52	Pathways	45:52	Large-Scale Identification and Fragmentation Pathways Analysis of N-Glycans from Mouse Brain.					
31098956	5	18	theme	permethylated	1132:1144	arg1	N-glycans					1146:1154	permethylated N-glycans	1132:1154	permethylated N-glycans in the positive mode	1132:1175	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	4	19	dep	modes	841:845	arg1	both					806:809	both	806:809	both	806:809	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	4	20	theme	unique	931:936	arg1	N-glycans					938:946	221 unique N-glycans	927:946	221 unique N-glycans with putative topological structures	927:983	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	6	21	theme	general	1349:1355	arg1	search					1366:1371	general database search	1349:1371	general database search of tandem mass spectra of N-glycans	1349:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	1	22	theme	common	136:141	arg1	PTMs					151:154	the most common protein PTMs	127:154	the most common protein PTMs	127:154	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	2	23	theme	ions	540:543	arg1	characteristics					513:527	characteristics	513:527	characteristics of product ions	513:543	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	2	23	theme	ions	540:543	arg1	understanding					469:481	comprehensive understanding	455:481	comprehensive understanding of fragmentation pathways	455:507	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	24	theme	reported	1249:1256	arg1	N-glycans					1258:1266	The reported N-glycans	1245:1266	The reported N-glycans	1245:1266	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	6	24	theme	reported	1249:1256	arg1	reference					1285:1293	a basic reference	1277:1293	a basic reference for future glycosylation study of mouse brain	1277:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	2	25	used	used	387:390	arg2	spectrometry					358:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	1	26	theme	PTMs	151:154	arg1	PTMs					151:154	the most common protein PTMs	127:154	the most common protein PTMs	127:154	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	26	theme	PTMs	151:154	arg1	one					120:122	one	120:122	one	120:122	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	4	27	theme	best	1042:1045	arg1	hits					1047:1050	best hits	1042:1050	best hits of 1	1042:1055	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	6	28	theme	B/Y/Z	1410:1414	arg1	ions					1416:1419	B/Y/Z ions	1410:1419	B/Y/Z ions	1410:1419	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	29	theme	N-glycans	1188:1196	arg1	ions					1124:1127	product ions	1116:1127	product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1116:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	30	from	N-glycans	1146:1154	arg1	mode					1214:1217	the negative mode	1201:1217	the negative mode	1201:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	30	from	N-glycans	1146:1154	arg1	mode					1172:1175	the positive mode	1159:1175	the positive mode	1159:1175	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	2	31	theme	topological	396:406	arg1	characterization					418:433	topological structure characterization	396:433	topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification	396:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	32	theme	spectra	1388:1394	arg1	search					1366:1371	general database search	1349:1371	general database search of tandem mass spectra of N-glycans	1349:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	33	theme	positive	1163:1170	arg1	mode					1172:1175	the positive mode	1159:1175	the positive mode	1159:1175	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	0	34	theme	Large-Scale	0:10	arg1	Identification					12:25	Large-Scale Identification	0:25	Large-Scale Identification	0:25	Large-Scale Identification and Fragmentation Pathways Analysis of N-Glycans from Mouse Brain.					
31098956	6	35	from	form	1535:1538	arg1	mode					1556:1559	the negative mode	1543:1559	the negative mode	1543:1559	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	4	36	theme	LC-MS/MS	785:792	arg1	analysis					794:801	LC-MS/MS analysis	785:801	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	2	37	theme	N-glycans	438:446	arg1	characterization					418:433	topological structure characterization	396:433	topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification	396:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	38	theme	permethylated	1465:1477	arg1	form					1479:1482	the permethylated form	1461:1482	the permethylated form in the positive mode	1461:1503	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	3	39	theme	N-glycome	684:692	arg1	study					675:679	our glycomic study	662:679	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	3	39	theme	N-glycome	684:692	arg1	analysis					742:749	fragmentation pathway analysis	720:749	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	1	40	dep	structure	177:185	arg1	composition					203:213	monosaccharide composition	188:213	monosaccharide composition	188:213	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	40	dep	structure	177:185	arg1	linkages					250:257	glycosidic linkages	239:257	glycosidic linkages	239:257	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	40	dep	structure	177:185	arg1	sequence					219:226	sequence	219:226	sequence	219:226	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	2	41	theme	pathways	500:507	arg1	characteristics					513:527	characteristics	513:527	characteristics of product ions	513:543	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	2	41	theme	pathways	500:507	arg1	understanding					469:481	comprehensive understanding	455:481	comprehensive understanding of fragmentation pathways	455:507	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	42	from	ions	1515:1518	arg1	mode					1500:1503	the positive mode	1487:1503	the positive mode	1487:1503	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	2	43	theme	fragmentation	486:498	arg1	pathways					500:507	fragmentation pathways	486:507	fragmentation pathways	486:507	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	2	44	theme	Tandem	346:351	arg1	spectrometry					358:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry	346:369	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	4	45	dep	search	902:907	arg1	GlySeeker					916:924	engine GlySeeker	909:924	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	1	46	theme	biological	314:323	arg1	functions					325:333	their biological functions	308:333	their biological functions	308:333	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	5	47	theme	pathways	1084:1091	arg1	characteristics					1097:1111	characteristics	1097:1111	characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1097:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	47	theme	pathways	1084:1091	arg1	Analysis					1058:1065	Analysis	1058:1065	Analysis of fragmentation pathways	1058:1091	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	3	48	theme	mouse	697:701	arg1	brain					703:707	mouse brain	697:707	mouse brain	697:707	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	4	49	theme	ESI	837:839	arg1	modes					841:845	both the positive and negative ESI modes	806:845	modes	841:845	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	1	50	link	N-linked	94:101	arg1	glycosylation					103:115	N-linked glycosylation	94:115	N-linked glycosylation	94:115	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	3	51	theme	pathway	734:740	arg1	analysis					742:749	fragmentation pathway analysis	720:749	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	5	52	from	mode	1214:1217	arg1	ions					1124:1127	product ions	1116:1127	product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1116:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	6	53	theme	mouse	1329:1333	arg1	brain					1335:1339	mouse brain	1329:1339	mouse brain	1329:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	6	54	theme	positive	1491:1498	arg1	mode					1500:1503	the positive mode	1487:1503	the positive mode	1487:1503	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	3	55	theme	identified	758:767	arg1	N-glycans					769:777	the identified N-glycans	754:777	the identified N-glycans	754:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	6	56	theme	future	1299:1304	arg1	study					1320:1324	future glycosylation study	1299:1324	future glycosylation study of mouse brain	1299:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	4	57	theme	N-glycan	884:891	arg1	search					902:907	our recently developed N-glycan database search	861:907	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	6	58	theme	mass	1383:1386	arg1	spectra					1388:1394	tandem mass spectra	1376:1394	tandem mass spectra of N-glycans	1376:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	1	59	theme	monosaccharide	188:201	arg1	composition					203:213	monosaccharide composition	188:213	monosaccharide composition	188:213	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	4	60	theme	putative	953:960	arg1	structures					974:983	putative topological structures	953:983	putative topological structures	953:983	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	4	61	dep	together	847:854	arg1	with					856:859	with	856:859	with	856:859	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	2	62	theme	best	570:573	arg1	interpretation					575:588	best interpretation	570:588	best interpretation of MS/MS data and highest confidence of identification	570:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	63	theme	basic	1279:1283	arg1	N-glycans					1258:1266	The reported N-glycans	1245:1266	The reported N-glycans	1245:1266	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	6	63	theme	basic	1279:1283	arg1	reference					1285:1293	a basic reference	1277:1293	a basic reference for future glycosylation study of mouse brain	1277:1339	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	6	64	theme	database	1357:1364	arg1	search					1366:1371	general database search	1349:1371	general database search of tandem mass spectra of N-glycans	1349:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	65	theme	N-glycans	1146:1154	arg1	ions					1124:1127	product ions	1116:1127	product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1116:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	2	66	theme	MS/MS	593:597	arg1	data					599:602	MS/MS data	593:602	MS/MS data	593:602	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	4	67	from	modes	841:845	arg1	analysis					794:801	LC-MS/MS analysis	785:801	LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker	785:924	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	2	68	theme	data	599:602	arg1	interpretation					575:588	best interpretation	570:588	best interpretation of MS/MS data and highest confidence of identification	570:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	0	69	theme	Mouse	81:85	arg1	Brain					87:91	Mouse Brain	81:91	Mouse Brain	81:91	Large-Scale Identification and Fragmentation Pathways Analysis of N-Glycans from Mouse Brain.					
31098956	5	70	theme	product	1116:1122	arg1	ions					1124:1127	product ions	1116:1127	product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode	1116:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	1	71	theme	protein	143:149	arg1	PTMs					151:154	the most common protein PTMs	127:154	the most common protein PTMs	127:154	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	2	72	theme	product	532:538	arg1	ions					540:543	product ions	532:543	product ions	532:543	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	6	73	theme	N-glycans	1399:1407	arg1	spectra					1388:1394	tandem mass spectra	1376:1394	tandem mass spectra of N-glycans	1376:1407	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	74	theme	native	1181:1186	arg1	N-glycans					1188:1196	native N-glycans	1181:1196	native N-glycans in the negative mode	1181:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	6	75	from	form	1479:1482	arg1	mode					1500:1503	the positive mode	1487:1503	the positive mode	1487:1503	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	5	76	from	ions	1124:1127	arg1	mode					1214:1217	the negative mode	1201:1217	the negative mode	1201:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	76	from	ions	1124:1127	arg1	mode					1172:1175	the positive mode	1159:1175	the positive mode	1159:1175	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	4	77	theme	target-decoy	1006:1017	arg1	searches					1019:1026	target-decoy searches	1006:1026	target-decoy searches	1006:1026	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	1	78	theme	N-linked	94:101	arg1	glycosylation					103:115	N-linked glycosylation	94:115	N-linked glycosylation	94:115	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	2	79	theme	comprehensive	455:467	arg1	understanding					469:481	comprehensive understanding	455:481	comprehensive understanding of fragmentation pathways	455:507	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	1	80	theme	vital	276:280	arg1	information					282:292	vital information	276:292	vital information to understand their biological functions and roles	276:343	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	1	80	theme	vital	276:280	arg1	structure					177:185	the topological structure	161:185	the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans	161:271	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31098956	4	81	with	N-glycans	938:946	arg1	structures					974:983	putative topological structures	953:983	putative topological structures	953:983	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	5	82	from	N-glycans	1188:1196	arg1	mode					1214:1217	the negative mode	1201:1217	the negative mode	1201:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	82	from	N-glycans	1188:1196	arg1	mode					1172:1175	the positive mode	1159:1175	the positive mode	1159:1175	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	4	83	theme	hits	1047:1050	arg1	number					1032:1037	number	1032:1037	number of best hits of 1	1032:1055	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	4	83	theme	hits	1047:1050	arg1	searches					1019:1026	target-decoy searches	1006:1026	target-decoy searches	1006:1026	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	3	84	theme	glycomic	666:673	arg1	study					675:679	our glycomic study	662:679	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	2	85	theme	structure	408:416	arg1	characterization					418:433	topological structure characterization	396:433	topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification	396:643	Tandem mass spectrometry has been widely used for topological structure characterization of N-glycans, where comprehensive understanding of fragmentation pathways and characteristics of product ions are essential to achieve best interpretation of MS/MS data and highest confidence of identification.					
31098956	0	86	theme	N-Glycans	66:74	arg1	Analysis					54:61	Analysis	54:61	Analysis of N-Glycans	54:74	Large-Scale Identification and Fragmentation Pathways Analysis of N-Glycans from Mouse Brain.					
31098956	5	87	theme	negative	1205:1212	arg1	mode					1214:1217	the negative mode	1201:1217	the negative mode	1201:1217	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	5	88	theme	fragmentation	1070:1082	arg1	pathways					1084:1091	fragmentation pathways	1070:1091	fragmentation pathways	1070:1091	Analysis of fragmentation pathways and characteristics of product ions of permethylated N-glycans in the positive mode and native N-glycans in the negative mode were further carried out.					
31098956	4	89	theme	1	1055:1055	arg1	hits					1047:1050	best hits	1042:1050	best hits of 1	1042:1055	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	3	90	theme	brain	703:707	arg1	N-glycome					684:692	N-glycome	684:692	N-glycome of mouse brain	684:707	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	6	91	theme	native	1528:1533	arg1	form					1535:1538	the native form	1524:1538	the native form in the negative mode	1524:1559	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	4	92	theme	positive	815:822	arg1	modes					841:845	both the positive and negative ESI modes	806:845	modes	841:845	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	3	93	theme	fragmentation	720:732	arg1	analysis					742:749	fragmentation pathway analysis	720:749	our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans	662:777	Here, we report our glycomic study of N-glycome of mouse brain as well as fragmentation pathway analysis of the identified N-glycans.					
31098956	4	94	theme	negative	828:835	arg1	modes					841:845	both the positive and negative ESI modes	806:845	modes	841:845	With LC-MS/MS analysis at both the positive and negative ESI modes together with our recently developed N-glycan database search engine GlySeeker, 221 unique N-glycans with putative topological structures were identified with target-decoy searches and number of best hits of 1.					
31098956	6	95	theme	B/C/Z	1509:1513	arg1	ions					1515:1518	B/C/Z ions	1509:1518	B/C/Z ions for the native form in the negative mode	1509:1559	The reported N-glycans serve as a basic reference for future glycosylation study of mouse brain; and in general database search of tandem mass spectra of N-glycans, B/Y/Z ions should be preferentially considered for the permethylated form in the positive mode and B/C/Z ions for the native form in the negative mode.					
31098956	1	96	theme	glycosidic	239:248	arg1	linkages					250:257	glycosidic linkages	239:257	glycosidic linkages	239:257	N-linked glycosylation is one of the most common protein PTMs, and the topological structure (monosaccharide composition and sequence as well as glycosidic linkages) of N-glycans is vital information to understand their biological functions and roles.					
31025190	5	0	theme	carbohydrate	755:766	arg1	chains					768:773	the carbohydrate chains	751:773	the carbohydrate chains	751:773	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	5	1	from	sites	801:805	arg1	cytokine					814:821	the cytokine	810:821	the cytokine	810:821	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	6	2	theme	solvent-exposed	997:1011	arg1	tails					1051:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	5	3	theme	chains	768:773	arg1	interaction					736:746	the interaction	732:746	the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini	732:879	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	0	4	from	modeling	10:17	arg1	dynamics					72:79	dynamics	72:79	dynamics	72:79	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	0	4	from	modeling	10:17	arg1	structure					58:66	structure	58:66	structure	58:66	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	1	5	theme	N-glycosylation	149:163	arg1	sites					165:169	two N-glycosylation sites	145:169	two N-glycosylation sites in each monomer chain	145:191	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	0	6	theme	human	84:88	arg1	interferon-gamma					90:105	human interferon-gamma	84:105	human interferon-gamma	84:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	1	7	with	glycoprotein	127:138	arg1	sites					165:169	two N-glycosylation sites	145:169	two N-glycosylation sites in each monomer chain	145:191	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	5	8	theme	molecular	692:700	arg1	simulations					711:721	molecular dynamics simulations	692:721	molecular dynamics simulations	692:721	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	1	9	gly	glycosylated	237:248	arg1	hIFNγ					116:120	Natural hIFNγ	108:120	Natural hIFNγ	108:120	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	1	9	gly	glycosylated	237:248	arg1	glycoprotein					127:138	a glycoprotein	125:138	a glycoprotein	125:138	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	3	10	theme	model	479:483	arg1	structures					485:494	model structures	479:494	model structures of glycosylated full-length native hIFNγ homodimers	479:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	7	11	theme	C-termini	1096:1104	arg1	motion					1114:1119	the C-termini wagging motion	1092:1119	the C-termini wagging motion into the solvent	1092:1136	We show that the glycans restrict the C-termini wagging motion into the solvent, limit their flexibility and keep them closer to the α-helical globule of hIFNγ, thus possibly protecting them from proteolytic processing.					
31025190	3	12	gly	glycosylated	499:510	arg1	homodimers					537:546	glycosylated full-length native hIFNγ homodimers	499:546	glycosylated full-length native hIFNγ homodimers	499:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	4	13	theme	molecule	659:666	arg1	integrity					633:641	the integrity	629:641	the integrity of the cytokine molecule	629:666	Our aim is to shed light on the mechanism through which glycosylation preserves the integrity of the cytokine molecule.					
31025190	0	14	theme	interferon-gamma	90:105	arg1	dynamics					72:79	dynamics	72:79	dynamics	72:79	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	0	14	theme	interferon-gamma	90:105	arg1	structure					58:66	structure	58:66	structure	58:66	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	7	15	theme	wagging	1106:1112	arg1	motion					1114:1119	the C-termini wagging motion	1092:1119	the C-termini wagging motion into the solvent	1092:1136	We show that the glycans restrict the C-termini wagging motion into the solvent, limit their flexibility and keep them closer to the α-helical globule of hIFNγ, thus possibly protecting them from proteolytic processing.					
31025190	3	16	theme	homodimers	537:546	arg1	structures					485:494	model structures	479:494	model structures of glycosylated full-length native hIFNγ homodimers	479:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	2	17	theme	proteolytic	376:386	arg1	degradation					388:398	proteolytic degradation	376:398	proteolytic degradation	376:398	Although glycosylation is not necessary for the activity of the cytokine, it was proposed that it protects the cytokine from proteolytic degradation and thus extends its circulatory half-life.					
31025190	0	18	from	effects	26:32	arg1	dynamics					72:79	dynamics	72:79	dynamics	72:79	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	0	18	from	effects	26:32	arg1	structure					58:66	structure	58:66	structure	58:66	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	7	19	theme	hIFNγ	1212:1216	arg1	globule					1201:1207	the α-helical globule	1187:1207	the α-helical globule of hIFNγ	1187:1216	We show that the glycans restrict the C-termini wagging motion into the solvent, limit their flexibility and keep them closer to the α-helical globule of hIFNγ, thus possibly protecting them from proteolytic processing.					
31025190	2	20	theme	cytokine	315:322	arg1	activity					299:306	the activity	295:306	the activity of the cytokine	295:322	Although glycosylation is not necessary for the activity of the cytokine, it was proposed that it protects the cytokine from proteolytic degradation and thus extends its circulatory half-life.					
31025190	0	21	theme	Molecular	0:8	arg1	modeling					10:17	Molecular modeling	0:17	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma	0:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	5	22	theme	charged	863:869	arg1	C-termini					871:879	its flexible highly positively charged C-termini	832:879	its flexible highly positively charged C-termini	832:879	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	1	23	theme	monomer	179:185	arg1	chain					187:191	each monomer chain	174:191	each monomer chain	174:191	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	0	24	dep	structure	58:66	arg1	the					54:56	the	54:56	the	54:56	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	2	25	theme	circulatory	421:431	arg1	half-life					433:441	its circulatory half-life	417:441	its circulatory half-life	417:441	Although glycosylation is not necessary for the activity of the cytokine, it was proposed that it protects the cytokine from proteolytic degradation and thus extends its circulatory half-life.					
31025190	3	26	theme	hIFNγ	531:535	arg1	homodimers					537:546	glycosylated full-length native hIFNγ homodimers	499:546	glycosylated full-length native hIFNγ homodimers	499:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	0	27	from	dynamics	72:79	arg1	modeling					10:17	Molecular modeling	0:17	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma	0:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	3	28	theme	glycosylated	499:510	arg1	homodimers					537:546	glycosylated full-length native hIFNγ homodimers	499:546	glycosylated full-length native hIFNγ homodimers	499:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	6	29	theme	globular	922:929	arg1	part					931:934	the globular part	918:934	the globular part of the protein	918:949	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	7	30	theme	α-helical	1191:1199	arg1	globule					1201:1207	the α-helical globule	1187:1207	the α-helical globule of hIFNγ	1187:1216	We show that the glycans restrict the C-termini wagging motion into the solvent, limit their flexibility and keep them closer to the α-helical globule of hIFNγ, thus possibly protecting them from proteolytic processing.					
31025190	5	31	theme	dynamics	702:709	arg1	simulations					711:721	molecular dynamics simulations	692:721	molecular dynamics simulations	692:721	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	5	32	theme	flexible	836:843	arg1	C-termini					871:879	its flexible highly positively charged C-termini	832:879	its flexible highly positively charged C-termini	832:879	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	0	33	theme	effects	26:32	arg1	modeling					10:17	Molecular modeling	0:17	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma	0:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	0	34	from	structure	58:66	arg1	modeling					10:17	Molecular modeling	0:17	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma	0:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	5	35	with	interaction	736:746	arg1	sites					801:805	the receptor-binding sites	780:805	the receptor-binding sites in the cytokine	780:821	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	5	35	with	interaction	736:746	arg1	C-termini					871:879	its flexible highly positively charged C-termini	832:879	its flexible highly positively charged C-termini	832:879	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	6	36	theme	C-terminal	1040:1049	arg1	tails					1051:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	5	37	theme	receptor-binding	784:799	arg1	sites					801:805	the receptor-binding sites	780:805	the receptor-binding sites in the cytokine	780:821	To this end, we employ molecular dynamics simulations to study the interaction of the carbohydrate chains with the receptor-binding sites in the cytokine and with its flexible highly positively charged C-termini.					
31025190	6	38	theme	protein	943:949	arg1	part					931:934	the globular part	918:934	the globular part of the protein	918:949	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	1	39	theme	Natural	108:114	arg1	hIFNγ					116:120	Natural hIFNγ	108:120	Natural hIFNγ	108:120	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	1	39	theme	Natural	108:114	arg1	glycoprotein					127:138	a glycoprotein	125:138	a glycoprotein	125:138	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	0	40	theme	glycosylation	37:49	arg1	effects					26:32	the effects	22:32	the effects of glycosylation on the structure and dynamics of human interferon-gamma	22:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	1	41	gly	glycoprotein	127:138	arg1	hIFNγ					116:120	Natural hIFNγ	108:120	Natural hIFNγ	108:120	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	1	41	gly	glycoprotein	127:138	arg1	glycoprotein					127:138	a glycoprotein	125:138	a glycoprotein	125:138	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	3	42	theme	structures	485:494	arg1	development					464:474	the development	460:474	the development of model structures of glycosylated full-length native hIFNγ homodimers	460:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	1	43	gly	N-glycosylation	149:163	arg2	sites					165:169	two N-glycosylation sites	145:169	two N-glycosylation sites in each monomer chain	145:191	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	1	43	gly	N-glycosylation	149:163	arg2	two					145:147	two	145:147	two	145:147	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	7	44	theme	proteolytic	1254:1264	arg1	processing					1266:1275	proteolytic processing	1254:1275	proteolytic processing	1254:1275	We show that the glycans restrict the C-termini wagging motion into the solvent, limit their flexibility and keep them closer to the α-helical globule of hIFNγ, thus possibly protecting them from proteolytic processing.					
31025190	6	45	with	contacts	979:986	arg1	tails					1051:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	0	46	gly	glycosylation	37:49	arg1	interferon-gamma					90:105	human interferon-gamma	84:105	human interferon-gamma	84:105	Molecular modeling of the effects of glycosylation on the structure and dynamics of human interferon-gamma.					
31025190	3	47	theme	full-length	512:522	arg1	homodimers					537:546	glycosylated full-length native hIFNγ homodimers	499:546	glycosylated full-length native hIFNγ homodimers	499:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
31025190	4	48	theme	cytokine	650:657	arg1	molecule					659:666	the cytokine molecule	646:666	the cytokine molecule	646:666	Our aim is to shed light on the mechanism through which glycosylation preserves the integrity of the cytokine molecule.					
31025190	6	49	theme	sensitive	1017:1025	arg1	tails					1051:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	the solvent-exposed and sensitive to proteases C-terminal tails	993:1055	The glycans interact primarily with the globular part of the protein, but also occasionally form contacts with the solvent-exposed and sensitive to proteases C-terminal tails.					
31025190	1	50	from	sites	165:169	arg1	chain					187:191	each monomer chain	174:191	each monomer chain	174:191	Natural hIFNγ is a glycoprotein with two N-glycosylation sites in each monomer chain, which are independently and differentially glycosylated.					
31025190	3	51	theme	native	524:529	arg1	homodimers					537:546	glycosylated full-length native hIFNγ homodimers	499:546	glycosylated full-length native hIFNγ homodimers	499:546	Here, we report the development of model structures of glycosylated full-length native hIFNγ homodimers.					
29725729	2	0	theme	higher	469:474	arg1	sensitivity					476:486	sensitivity	476:486	sensitivity	476:486	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	4	1	theme	hits	1360:1363	arg1	rate					1331:1334	spectrum-level false discovery rate ≤ 1% and number	1300:1350	rate	1331:1334	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	4	1	theme	hits	1360:1363	arg1	number					1345:1350	number	1345:1350	number	1345:1350	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	2	theme	41Y41M	564:569	arg1	31M41Y41L41S61M41Y41L41S					571:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	0	3	theme	LO2	84:86	arg1	cells					88:92	LO2 cells	84:92	LO2 cells	84:92	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	1	4	dep	US	274:275	arg1	FDA					307:309	FDA	307:309	FDA	307:309	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	1	4	dep	US	274:275	arg1	Food					277:280	Food	277:280	Food	277:280	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	1	4	dep	US	274:275	arg1	Administration					291:304	Drug Administration	286:304	Drug Administration	286:304	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	5	5	theme	quantitative	1476:1487	arg1	analysis					1489:1496	further quantitative analysis	1468:1496	further quantitative analysis	1468:1496	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	4	6	theme	N-glycome	1177:1185	arg1	characterization					1145:1160	our large-scale characterization	1129:1160	our large-scale characterization of human liver N-glycome with primary structures	1129:1209	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	1	7	theme	pathological	174:185	arg1	disorders					187:195	various physiological and pathological disorders	148:195	various physiological and pathological disorders (including cancers)	148:215	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	5	8	theme	markers	1597:1603	arg1	N-glycans					1555:1563	differentially expressed N-glycans	1530:1563	differentially expressed N-glycans	1530:1563	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	8	theme	markers	1597:1603	arg1	discovery					1569:1577	discovery	1569:1577	discovery	1569:1577	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	1	9	theme	early	327:331	arg1	diagnosis					333:341	early diagnosis	327:341	early diagnosis	327:341	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	2	10	theme	61F	560:562	arg1	31M41Y41L41S61M41Y41L41S					571:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	3	11	theme	database	1053:1060	arg1	search					1062:1067	our recently developed N-glycan database search	1021:1067	our recently developed N-glycan database search	1021:1067	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	4	12	dep	rate	1331:1334	arg1	%					1339:1339	≤ 1%	1336:1339	≤ 1%	1336:1339	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	3	13	dep	information	946:956	arg1	linkage					972:978	linkage	972:978	linkage	972:978	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	13	dep	information	946:956	arg1	sequence					959:966	sequence	959:966	sequence	959:966	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	2	14	theme	diagnostic	609:618	arg1	value					620:624	bigger diagnostic value	602:624	bigger diagnostic value for hepatocellular carcinoma	602:653	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	3	15	theme	various	850:856	arg1	glycoforms					858:867	various glycoforms	850:867	various glycoforms	850:867	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	16	theme	comprehensive	921:933	arg1	information					946:956	comprehensive structural information	921:956	not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans	876:992	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	17	theme	high-throughput	814:828	arg1	characterization					830:845	high-throughput characterization	814:845	high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker	814:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	5	18	theme	N-glycome	1447:1455	arg1	study					1457:1461	future liver N-glycome study	1434:1461	future liver N-glycome study	1434:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	2	19	theme	glycoform	522:530	arg1	AFP-L3					532:537	AFP glycoform AFP-L3	518:537	AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	518:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	1	20	gly	glycoproteins	234:246	arg1	glycoproteins					234:246	quite a few glycoproteins	222:246	quite a few glycoproteins	222:246	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	1	20	gly	glycoproteins	234:246	arg1	approved					258:265	approved	258:265	approved	258:265	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	2	21	dep	combination	677:687	arg1	i.e.					671:674	i.e.	671:674	i.e.	671:674	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	1	22	gly	glycosylation	104:116	arg1	disorders					187:195	various physiological and pathological disorders	148:195	various physiological and pathological disorders (including cancers)	148:215	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	4	23	theme	best	1355:1358	arg1	hits					1360:1363	best hits	1355:1363	best hits of 1	1355:1368	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	5	24	theme	further	1468:1474	arg1	analysis					1489:1496	further quantitative analysis	1468:1496	further quantitative analysis	1468:1496	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	2	25	theme	total	660:664	arg1	AFP					666:668	total AFP	660:668	total AFP (i.e., combination of all glycoforms)	660:706	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	4	26	theme	human	1165:1169	arg1	N-glycome					1177:1185	human liver N-glycome	1165:1185	human liver N-glycome	1165:1185	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	27	theme	multiple	371:378	arg1	glycoforms					380:389	multiple glycoforms	371:389	multiple glycoforms	371:389	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	4	28	theme	unique	1238:1243	arg1	structures					1253:1262	unique primary structures	1238:1262	unique primary structures	1238:1262	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	29	theme	31M41Y41L41S61M41Y41L41S	571:594	arg1	AFP-L3					532:537	AFP glycoform AFP-L3	518:537	AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	518:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	3	30	theme	engine	1069:1074	arg1	GlySeeker					1076:1084	thanks to our recently developed N-glycan database search engine GlySeeker	1011:1084	thanks to our recently developed N-glycan database search engine GlySeeker	1011:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	0	31	theme	Large-scale	0:10	arg1	identification					12:25	identification	12:25	identification	12:25	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	5	32	theme	LO2	1375:1377	arg1	N-glycans					1379:1387	The LO2 N-glycans	1371:1387	The LO2 N-glycans reported here	1371:1401	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	32	theme	LO2	1375:1377	arg1	characterization					1510:1525	characterization	1510:1525	characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases	1510:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	32	theme	LO2	1375:1377	arg1	reference					1420:1428	a basic reference	1412:1428	a basic reference for future liver N-glycome study	1412:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	3	33	theme	N-glycan	1044:1051	arg1	search					1062:1067	our recently developed N-glycan database search	1021:1067	our recently developed N-glycan database search	1021:1067	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	1	34	theme	Aberrant	95:102	arg1	glycosylation					104:116	Aberrant glycosylation	95:116	Aberrant glycosylation	95:116	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	0	35	theme	liver	54:58	arg1	N-glycome					60:68	human liver N-glycome	48:68	human liver N-glycome	48:68	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	4	36	theme	discovery	1321:1329	arg1	rate					1331:1334	spectrum-level false discovery rate ≤ 1% and number	1300:1350	rate	1331:1334	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	3	37	theme	spectrometry-based	714:731	arg1	pipeline					801:808	the state-of-the-art instrumental analytical pipeline	756:808	the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker	756:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	37	theme	spectrometry-based	714:731	arg1	glycomics					733:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	5	38	theme	discovery	1569:1577	arg1	N-glycans					1379:1387	The LO2 N-glycans	1371:1387	The LO2 N-glycans reported here	1371:1401	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	38	theme	discovery	1569:1577	arg1	characterization					1510:1525	characterization	1510:1525	characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases	1510:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	38	theme	discovery	1569:1577	arg1	reference					1420:1428	a basic reference	1412:1428	a basic reference for future liver N-glycome study	1412:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	2	39	contain	have	459:462	arg1	glycoforms					442:451	only some specific glycoforms	423:451	only some specific glycoforms which have much higher sensitivity and specificity	423:502	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	39	contain	have	459:462	arg2	specificity					492:502	specificity	492:502	specificity	492:502	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	39	contain	have	459:462	arg2	sensitivity					476:486	sensitivity	476:486	sensitivity	476:486	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	40	theme	bigger	602:607	arg1	value					620:624	bigger diagnostic value	602:624	bigger diagnostic value for hepatocellular carcinoma	602:653	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	5	41	theme	liver	1441:1445	arg1	study					1457:1461	future liver N-glycome study	1434:1461	future liver N-glycome study	1434:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	4	42	theme	liver	1171:1175	arg1	N-glycome					1177:1185	human liver N-glycome	1165:1185	human liver N-glycome	1165:1185	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	43	gly	glycoprotein	349:360	arg1	glycoprotein					349:360	Each glycoprotein	344:360	Each glycoprotein	344:360	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	44	theme	cancer-related	396:409	arg1	ones					411:414	cancer-related ones	396:414	cancer-related ones	396:414	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	0	45	theme	human	48:52	arg1	N-glycome					60:68	human liver N-glycome	48:68	human liver N-glycome	48:68	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	1	46	theme	Drug	286:289	arg1	Administration					291:304	Drug Administration	286:304	Drug Administration	286:304	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	2	47	theme	AFP	518:520	arg1	AFP-L3					532:537	AFP glycoform AFP-L3	518:537	AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	518:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	48	contain	have	366:369	arg2	glycoforms					380:389	multiple glycoforms	371:389	multiple glycoforms	371:389	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	48	contain	have	366:369	arg1	glycoprotein					349:360	Each glycoprotein	344:360	Each glycoprotein	344:360	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	5	49	theme	effective	1587:1595	arg1	markers					1597:1603	more effective markers	1582:1603	more effective markers for liver and other diseases	1582:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	4	50	theme	1	1368:1368	arg1	hits					1360:1363	best hits	1355:1363	best hits of 1	1355:1368	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	3	51	theme	structural	935:944	arg1	information					946:956	comprehensive structural information	921:956	not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans	876:992	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	4	52	with	characterization	1145:1160	arg1	structures					1200:1209	primary structures	1192:1209	primary structures	1192:1209	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	0	53	theme	N-glycome	60:68	arg1	visualization					31:43	visualization	31:43	visualization	31:43	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	0	53	theme	N-glycome	60:68	arg1	identification					12:25	identification	12:25	identification	12:25	Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells.					
29725729	4	54	theme	large-scale	1133:1143	arg1	characterization					1145:1160	our large-scale characterization	1129:1160	our large-scale characterization of human liver N-glycome with primary structures	1129:1209	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	55	theme	01Y	556:558	arg1	31M41Y41L41S61M41Y41L41S					571:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S	556:594	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	1	56	dep	disorders	187:195	arg1	including					198:206	including	198:206	including cancers	198:214	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	3	57	theme	N-glycans	984:992	arg1	information					946:956	comprehensive structural information	921:956	not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans	876:992	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	57	theme	N-glycans	984:992	arg1	composition					900:910	monosaccharide composition	885:910	not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans	876:992	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	4	58	with	N-glycans	1223:1231	arg1	structures					1253:1262	unique primary structures	1238:1262	unique primary structures	1238:1262	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	1	59	located	observed	136:143	arg1	disorders					187:195	various physiological and pathological disorders	148:195	various physiological and pathological disorders (including cancers)	148:215	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	1	59	located	observed	136:143	arg2	glycosylation					104:116	Aberrant glycosylation	95:116	Aberrant glycosylation	95:116	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	5	60	theme	expressed	1545:1553	arg1	N-glycans					1555:1563	differentially expressed N-glycans	1530:1563	differentially expressed N-glycans	1530:1563	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	2	61	theme	specific	433:440	arg1	glycoforms					442:451	only some specific glycoforms	423:451	only some specific glycoforms which have much higher sensitivity and specificity	423:502	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	4	62	theme	unique	1216:1221	arg1	N-glycans					1223:1231	214 unique N-glycans	1212:1231	214 unique N-glycans with unique primary structures	1212:1262	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	63	theme	hepatocellular	630:643	arg1	carcinoma					645:653	hepatocellular carcinoma	630:653	hepatocellular carcinoma	630:653	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	5	64	theme	N-glycans	1555:1563	arg1	N-glycans					1379:1387	The LO2 N-glycans	1371:1387	The LO2 N-glycans reported here	1371:1401	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	64	theme	N-glycans	1555:1563	arg1	characterization					1510:1525	characterization	1510:1525	characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases	1510:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	64	theme	N-glycans	1555:1563	arg1	reference					1420:1428	a basic reference	1412:1428	a basic reference for future liver N-glycome study	1412:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	65	theme	basic	1414:1418	arg1	N-glycans					1379:1387	The LO2 N-glycans	1371:1387	The LO2 N-glycans reported here	1371:1401	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	65	theme	basic	1414:1418	arg1	characterization					1510:1525	characterization	1510:1525	characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases	1510:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	5	65	theme	basic	1414:1418	arg1	reference					1420:1428	a basic reference	1412:1428	a basic reference for future liver N-glycome study	1412:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	3	66	theme	developed	1034:1042	arg1	search					1062:1067	our recently developed N-glycan database search	1021:1067	our recently developed N-glycan database search	1021:1067	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	4	67	theme	new	1097:1099	arg1	capability					1101:1110	this new capability	1092:1110	this new capability	1092:1110	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	3	68	theme	analytical	790:799	arg1	pipeline					801:808	the state-of-the-art instrumental analytical pipeline	756:808	the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker	756:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	68	theme	analytical	790:799	arg1	glycomics					733:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	4	69	theme	false	1315:1319	arg1	rate					1331:1334	spectrum-level false discovery rate ≤ 1% and number	1300:1350	rate	1331:1334	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	2	70	theme	glycoforms	696:705	arg1	combination					677:687	combination	677:687	combination of all glycoforms	677:705	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	2	71	with	AFP-L3	532:537	arg1	N-glycan					544:551	N-glycan	544:551	N-glycan	544:551	Each glycoprotein may have multiple glycoforms, and cancer-related ones can be only some specific glycoforms which have much higher sensitivity and specificity; for example, AFP glycoform AFP-L3 with N-glycan of 01Y(61F)41Y41M(31M41Y41L41S61M41Y41L41S is of bigger diagnostic value for hepatocellular carcinoma than total AFP (i.e., combination of all glycoforms).					
29725729	1	72	theme	few	230:232	arg1	glycoproteins					234:246	quite a few glycoproteins	222:246	quite a few glycoproteins	222:246	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	1	72	theme	few	230:232	arg1	approved					258:265	approved	258:265	approved	258:265	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	5	73	theme	other	1619:1623	arg1	diseases					1625:1632	other diseases	1619:1632	other diseases	1619:1632	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	4	74	theme	spectrum-level	1300:1313	arg1	rate					1331:1334	spectrum-level false discovery rate ≤ 1% and number	1300:1350	rate	1331:1334	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	5	75	theme	future	1434:1439	arg1	study					1457:1461	future liver N-glycome study	1434:1461	future liver N-glycome study	1434:1461	The LO2 N-glycans reported here serve as a basic reference for future liver N-glycome study, and further quantitative analysis will enable characterization of differentially expressed N-glycans and discovery of more effective markers for liver and other diseases.					
29725729	3	76	theme	thanks	1011:1016	arg1	GlySeeker					1076:1084	thanks to our recently developed N-glycan database search engine GlySeeker	1011:1084	thanks to our recently developed N-glycan database search engine GlySeeker	1011:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	1	77	theme	various	148:154	arg1	disorders					187:195	various physiological and pathological disorders	148:195	various physiological and pathological disorders (including cancers)	148:215	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	3	78	theme	glycoforms	858:867	arg1	characterization					830:845	high-throughput characterization	814:845	high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker	814:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	6	79	with	ProteomeXchange	1658:1672	arg1	identifier					1679:1688	identifier PXD008158	1679:1698	identifier PXD008158	1679:1698	Data are available via ProteomeXchange with identifier PXD008158.					
29725729	4	80	theme	primary	1245:1251	arg1	structures					1253:1262	unique primary structures	1238:1262	unique primary structures	1238:1262	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	4	81	theme	primary	1192:1198	arg1	structures					1200:1209	primary structures	1192:1209	primary structures	1192:1209	With this new capability, here, we report our large-scale characterization of human liver N-glycome with primary structures; 214 unique N-glycans with unique primary structures were identified and visualized with spectrum-level false discovery rate ≤ 1% and number of best hits of 1.					
29725729	1	82	theme	physiological	156:168	arg1	disorders					187:195	various physiological and pathological disorders	148:195	various physiological and pathological disorders (including cancers)	148:215	Aberrant glycosylation has been commonly observed in various physiological and pathological disorders (including cancers), and quite a few glycoproteins have been approved by the US Food and Drug Administration (FDA) as markers for early diagnosis.					
29725729	3	83	theme	monosaccharide	885:898	arg1	composition					900:910	monosaccharide composition	885:910	not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans	876:992	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	84	theme	instrumental	777:788	arg1	pipeline					801:808	the state-of-the-art instrumental analytical pipeline	756:808	the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker	756:1084	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
29725729	3	84	theme	instrumental	777:788	arg1	glycomics					733:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics	709:741	Mass spectrometry-based glycomics is currently the state-of-the-art instrumental analytical pipeline for high-throughput characterization of various glycoforms, where not only monosaccharide composition but also comprehensive structural information (sequence and linkage) of N-glycans are now reported thanks to our recently developed N-glycan database search engine GlySeeker.					
30033600	1	0	theme	glycosylation	176:188	arg1	analysis					155:162	the analysis	151:162	the analysis of O-GalNAc glycosylation	151:188	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	0	1	theme	Simplification	88:101	arg1	Approach					103:110	an Acid-Assisted Glycoform Simplification Approach	61:110	an Acid-Assisted Glycoform Simplification Approach	61:110	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	1	2	theme	glycosidases	274:285	arg1	lack					266:269	lack	266:269	lack of glycosidases to release O-GalNAc glycans	266:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	1	2	theme	glycosidases	274:285	arg1	glycans					254:260	glycans	254:260	glycans	254:260	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	7	3	theme	structures	1198:1207	arg1	variations					1177:1186	the variations	1173:1186	the variations of glycan structures	1173:1207	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	6	4	theme	peptide	1000:1006	arg1	sequences					1008:1016	185 O-GalNAc modified peptide sequences	978:1016	185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%)	978:1099	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	7	5	theme	samples	1260:1266	arg1	analysis					1231:1238	analysis	1231:1238	analysis of other biological samples with high complexity	1231:1287	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	2	6	gly	glycosylation	450:462	arg1	serum					473:477	human serum	467:477	human serum	467:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	2	7	theme	HILIC	379:383	arg1	enrichment					385:394	HILIC enrichment	379:394	HILIC enrichment	379:394	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	1	8	theme	release	290:296	arg1	glycans					307:313	release O-GalNAc glycans	290:313	release O-GalNAc glycans	290:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	7	9	with	samples	1260:1266	arg1	complexity					1278:1287	high complexity	1273:1287	high complexity	1273:1287	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	5	10	theme	identified	823:832	arg1	backbones					842:850	identified peptide backbones	823:850	identified peptide backbones	823:850	Indeed, the number of identified peptide backbones increases nearly one fold when this strategy is used.					
30033600	3	11	theme	test	558:561	arg1	sample					563:568	the test sample	554:568	the test sample	554:568	This method is first validated by using the bovine fetuin as the test sample.					
30033600	6	12	theme	modified	991:998	arg1	sequences					1008:1016	185 O-GalNAc modified peptide sequences	978:1016	185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%)	978:1099	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	4	13	theme	bovine	650:655	arg1	fetuin					657:662	bovine fetuin	650:662	bovine fetuin	650:662	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	0	14	theme	Efficient	7:15	arg1	Identification					17:30	Highly Efficient Identification	0:30	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach	0:110	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	6	15	theme	O-GalNAc	982:989	arg1	sequences					1008:1016	185 O-GalNAc modified peptide sequences	978:1016	185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%)	978:1099	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	5	16	used	used	900:903	arg2	strategy					888:895	this strategy	883:895	this strategy	883:895	Indeed, the number of identified peptide backbones increases nearly one fold when this strategy is used.					
30033600	6	17	theme	false	1074:1078	arg1	rate					1090:1093	false detection rate	1074:1093	false detection rate	1074:1093	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	5	18	theme	peptide	834:840	arg1	backbones					842:850	identified peptide backbones	823:850	identified peptide backbones	823:850	Indeed, the number of identified peptide backbones increases nearly one fold when this strategy is used.					
30033600	1	19	theme	O-GalNAc	298:305	arg1	glycans					307:313	release O-GalNAc glycans	290:313	release O-GalNAc glycans	290:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	4	20	theme	glycan	743:748	arg1	structure					750:758	the glycan structure	739:758	the glycan structure	739:758	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	2	21	theme	chemical	401:408	arg1	de-sialylation					410:423	chemical de-sialylation	401:423	chemical de-sialylation	401:423	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	5	22	theme	backbones	842:850	arg1	number					813:818	the number	809:818	the number of identified peptide backbones	809:850	Indeed, the number of identified peptide backbones increases nearly one fold when this strategy is used.					
30033600	2	23	theme	human	467:471	arg1	serum					473:477	human serum	467:477	human serum	467:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	2	24	theme	serum	473:477	arg1	glycosylation					450:462	O-GalNAc glycosylation	441:462	O-GalNAc glycosylation of human serum	441:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	6	25	dep	FDR	1069:1071	arg1	%					1098:1098	<1%	1096:1098	<1%	1096:1098	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	6	25	dep	FDR	1069:1071	arg1	rate					1090:1093	false detection rate	1074:1093	false detection rate	1074:1093	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	1	26	theme	N-linked	127:134	arg1	glycosylation					136:148	N-linked glycosylation	127:148	N-linked glycosylation	127:148	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	1	27	theme	high	226:229	arg1	diversity					241:249	the high structure diversity	222:249	the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans	222:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	0	28	theme	Glycosylation	44:56	arg1	Identification					17:30	Highly Efficient Identification	0:30	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach	0:110	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	7	29	theme	other	1243:1247	arg1	samples					1260:1266	other biological samples	1243:1266	other biological samples with high complexity	1243:1287	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	1	30	theme	structure	231:239	arg1	diversity					241:249	the high structure diversity	222:249	the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans	222:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	0	31	theme	O-GalNAc	35:42	arg1	Glycosylation					44:56	O-GalNAc Glycosylation	35:56	O-GalNAc Glycosylation	35:56	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	4	32	theme	identification	773:786	arg1	sensitivity					788:798	identification sensitivity	773:798	identification sensitivity	773:798	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	2	33	theme	O-GalNAc	441:448	arg1	glycosylation					450:462	O-GalNAc glycosylation	441:462	O-GalNAc glycosylation of human serum	441:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	7	34	theme	straight	1122:1129	arg1	strategy					1139:1146	This straight forward strategy	1117:1146	This straight forward strategy	1117:1146	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	3	35	theme	bovine	537:542	arg1	fetuin					544:549	the bovine fetuin	533:549	the bovine fetuin as the test sample	533:568	This method is first validated by using the bovine fetuin as the test sample.					
30033600	0	36	theme	Acid-Assisted	64:76	arg1	Approach					103:110	an Acid-Assisted Glycoform Simplification Approach	61:110	an Acid-Assisted Glycoform Simplification Approach	61:110	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	7	37	theme	high	1273:1276	arg1	complexity					1278:1287	high complexity	1273:1287	high complexity	1273:1287	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	7	38	theme	forward	1131:1137	arg1	strategy					1139:1146	This straight forward strategy	1117:1146	This straight forward strategy	1117:1146	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	6	39	theme	human	952:956	arg1	sample					964:969	the human serum sample	948:969	the human serum sample	948:969	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	7	40	theme	glycan	1191:1196	arg1	structures					1198:1207	glycan structures	1191:1207	glycan structures	1191:1207	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	6	41	with	proteins	1038:1045	arg1	FDR					1069:1071	FDR	1069:1071	FDR	1069:1071	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	6	41	with	proteins	1038:1045	arg1	confidence					1057:1066	high confidence	1052:1066	high confidence (FDR (false detection rate) <1%)	1052:1099	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	7	42	theme	biological	1249:1258	arg1	samples					1260:1266	other biological samples	1243:1266	other biological samples with high complexity	1243:1287	This straight forward strategy can significantly reduce the variations of glycan structures, and is applicable to analysis of other biological samples with high complexity.					
30033600	4	43	theme	acid-assisted	671:683	arg1	method					700:705	the acid-assisted de-sialylation method	667:705	the acid-assisted de-sialylation method	667:705	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	2	44	theme	simplification	342:355	arg1	strategy					357:364	a glycoform simplification strategy	330:364	a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum	330:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	4	45	theme	sialic	609:614	arg1	residues					621:628	the sialic acid residues	605:628	the sialic acid residues	605:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	4	46	attach	removed	637:643	arg2	residues					621:628	the sialic acid residues	605:628	the sialic acid residues	605:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	4	46	attach	removed	637:643	arg1	fetuin					657:662	bovine fetuin	650:662	bovine fetuin	650:662	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	4	46	attach	removed	637:643	arg2	%					600:600	more than 90%	588:600	more than 90% of the sialic acid residues	588:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	2	47	theme	glycoform	332:340	arg1	strategy					357:364	a glycoform simplification strategy	330:364	a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum	330:477	In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to characterize O-GalNAc glycosylation of human serum is presented.					
30033600	1	48	theme	glycans	254:260	arg1	diversity					241:249	the high structure diversity	222:249	the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans	222:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	0	49	theme	Glycoform	78:86	arg1	Approach					103:110	an Acid-Assisted Glycoform Simplification Approach	61:110	an Acid-Assisted Glycoform Simplification Approach	61:110	Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.					
30033600	6	50	theme	high	1052:1055	arg1	FDR					1069:1071	FDR	1069:1071	FDR	1069:1071	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	6	50	theme	high	1052:1055	arg1	confidence					1057:1066	high confidence	1052:1066	high confidence (FDR (false detection rate) <1%)	1052:1099	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	4	51	theme	de-sialylation	685:698	arg1	method					700:705	the acid-assisted de-sialylation method	667:705	the acid-assisted de-sialylation method	667:705	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	6	52	theme	detection	1080:1088	arg1	rate					1090:1093	false detection rate	1074:1093	false detection rate	1074:1093	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	4	53	theme	residues	621:628	arg1	%					600:600	more than 90%	588:600	more than 90% of the sialic acid residues	588:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	4	53	theme	residues	621:628	arg1	residues					621:628	the sialic acid residues	605:628	the sialic acid residues	605:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	1	54	link	N-linked	127:134	arg1	glycosylation					136:148	N-linked glycosylation	127:148	N-linked glycosylation	127:148	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	4	55	theme	acid	616:619	arg1	residues					621:628	the sialic acid residues	605:628	the sialic acid residues	605:628	It is found that more than 90% of the sialic acid residues can be removed from bovine fetuin by the acid-assisted de-sialylation method, which significantly simplifies the glycan structure and improves identification sensitivity.					
30033600	1	56	theme	O-GalNAc	167:174	arg1	glycosylation					176:188	O-GalNAc glycosylation	167:188	O-GalNAc glycosylation	167:188	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
30033600	6	57	theme	serum	958:962	arg1	sample					964:969	the human serum sample	948:969	the human serum sample	948:969	This method is further applied to analyze the human serum sample, where 185 O-GalNAc modified peptide sequences corresponding to 94 proteins with high confidence (FDR (false detection rate) <1%) are identified.					
30033600	1	58	theme	lack	266:269	arg1	diversity					241:249	the high structure diversity	222:249	the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans	222:313	Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans.					
31719144	4	0	theme	large	523:527	arg1	portion					529:535	a large portion	521:535	a large portion	521:535	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	11	1	theme	occupancy	1330:1338	arg1	levels					1340:1345	the relative occupancy levels	1317:1345	the relative occupancy levels of the glycans	1317:1360	We quantified the relative occupancy levels of the glycans at each of the five sites and observed complex heterogeneity and site-specific glycosylation.					
31719144	1	2	theme	neutrophils	190:200	arg1	components					176:185	lysosomal components	166:185	lysosomal components of neutrophils	166:200	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	3	3	theme	abundant	349:356	arg1	MPO					339:341	MPO	339:341	MPO	339:341	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	3	theme	abundant	349:356	arg1	protein					382:388	an abundant neutrophil-derived heme protein	346:388	an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system	346:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	3	theme	abundant	349:356	arg1	part					398:401	part	398:401	part of the antimicrobial defense system	398:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	9	4	attach	attached	1131:1138	arg1	MPO					1143:1145	MPO	1143:1145	MPO	1143:1145	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	9	4	attach	attached	1131:1138	arg2	glycans					1123:1129	the glycans	1119:1129	the glycans attached to MPO	1119:1145	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	11	5	theme	relative	1321:1328	arg1	levels					1340:1345	the relative occupancy levels	1317:1345	the relative occupancy levels of the glycans	1317:1360	We quantified the relative occupancy levels of the glycans at each of the five sites and observed complex heterogeneity and site-specific glycosylation.					
31719144	9	6	theme	relative	1097:1104	arg1	abundance					1106:1114	relative abundance	1097:1114	relative abundance	1097:1114	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	13	7	theme	antigen-presenting	1858:1875	arg1	cells					1877:1881	antigen-presenting cells	1858:1881	antigen-presenting cells	1858:1881	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	2	8	theme	vessel	318:323	arg1	vasculitides					325:336	small vessel vasculitides	312:336	small vessel vasculitides	312:336	ANCAs directed to proteinase 3 and myeloperoxidase (MPO) in particular are associated with distinct forms of small vessel vasculitides.					
31719144	8	9	dep	sites	984:988	arg1	10					991:992	10	991:992	10	991:992	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	12	10	theme	high-mannose	1606:1617	arg1	species					1619:1625	phosphorylated high-mannose species	1591:1625	phosphorylated high-mannose species	1591:1625	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	2	11	theme	small	312:316	arg1	vasculitides					325:336	small vessel vasculitides	312:336	small vessel vasculitides	312:336	ANCAs directed to proteinase 3 and myeloperoxidase (MPO) in particular are associated with distinct forms of small vessel vasculitides.					
31719144	0	12	theme	Neutrophil	0:9	arg1	myeloperoxidase					11:25	Neutrophil myeloperoxidase	0:25	Neutrophil myeloperoxidase	0:25	Neutrophil myeloperoxidase harbors distinct site-specific peculiarities in its glycosylation.					
31719144	6	13	theme	specific	759:766	arg1	sites					768:772	specific sites	759:772	specific sites	759:772	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	6	14	theme	complex	741:747	arg1	sugars					749:754	complex sugars	741:754	complex sugars	741:754	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	4	15	theme	neutrophils	504:514	arg1	granules					492:499	the azurophilic granules	476:499	the azurophilic granules of neutrophils	476:514	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	5	16	theme	autoimmune	650:659	arg1	responses					661:669	antibody-mediated autoimmune responses	632:669	antibody-mediated autoimmune responses	632:669	It remains unclear why MPO is frequently the target of antibody-mediated autoimmune responses.					
31719144	5	17	theme	antibody-mediated	632:648	arg1	responses					661:669	antibody-mediated autoimmune responses	632:669	antibody-mediated autoimmune responses	632:669	It remains unclear why MPO is frequently the target of antibody-mediated autoimmune responses.					
31719144	1	18	theme	Anti-neutrophil	94:108	arg1	ANCAs					138:142	ANCAs	138:142	ANCAs	138:142	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	1	18	theme	Anti-neutrophil	94:108	arg1	autoantibodies					122:135	Anti-neutrophil cytoplasmic autoantibodies	94:135	Anti-neutrophil cytoplasmic autoantibodies (ANCAs)	94:143	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	12	19	theme	phosphorylated	1591:1604	arg1	species					1619:1625	phosphorylated high-mannose species	1591:1625	phosphorylated high-mannose species	1591:1625	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	5	20	theme	responses	661:669	arg1	target					622:627	the target	618:627	the target of antibody-mediated autoimmune responses	618:669	It remains unclear why MPO is frequently the target of antibody-mediated autoimmune responses.					
31719144	5	20	theme	responses	661:669	arg1	MPO					600:602	MPO	600:602	MPO	600:602	It remains unclear why MPO is frequently the target of antibody-mediated autoimmune responses.					
31719144	1	21	theme	cytoplasmic	110:120	arg1	ANCAs					138:142	ANCAs	138:142	ANCAs	138:142	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	1	21	theme	cytoplasmic	110:120	arg1	autoantibodies					122:135	Anti-neutrophil cytoplasmic autoantibodies	94:135	Anti-neutrophil cytoplasmic autoantibodies (ANCAs)	94:143	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	9	22	theme	glycans	1123:1129	arg1	scope					1087:1091	full scope	1082:1091	full scope	1082:1091	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	9	22	theme	glycans	1123:1129	arg1	abundance					1106:1114	relative abundance	1097:1114	relative abundance	1097:1114	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	8	23	from	10	991:992	arg1	dimer					1008:1012	the mature dimer	997:1012	the mature dimer	997:1012	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	3	24	theme	antimicrobial	410:422	arg1	system					432:437	the antimicrobial defense system	406:437	the antimicrobial defense system	406:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	12	25	theme	glycosylation	1483:1495	arg1	phenotypes					1497:1506	glycosylation phenotypes	1483:1506	glycosylation phenotypes uncommon for glycoproteins	1483:1533	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	11	26	theme	site-specific	1427:1439	arg1	glycosylation					1441:1453	site-specific glycosylation	1427:1453	site-specific glycosylation	1427:1453	We quantified the relative occupancy levels of the glycans at each of the five sites and observed complex heterogeneity and site-specific glycosylation.					
31719144	8	27	theme	putative	959:966	arg1	sites					984:988	5 putative N-glycosylation sites	957:988	5 putative N-glycosylation sites (10 in the mature dimer)	957:1013	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	6	28	gly	glycoprotein	693:704	arg1	MPO					672:674	MPO	672:674	MPO	672:674	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	6	28	gly	glycoprotein	693:704	arg1	glycoprotein					693:704	a homodimeric glycoprotein	679:704	a homodimeric glycoprotein	679:704	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	3	29	theme	defense	424:430	arg1	system					432:437	the antimicrobial defense system	406:437	the antimicrobial defense system	406:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	9	30	dep	scope	1087:1091	arg1	the					1078:1080	the	1078:1080	the	1078:1080	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	11	31	theme	glycans	1354:1360	arg1	levels					1340:1345	the relative occupancy levels	1317:1345	the relative occupancy levels of the glycans	1317:1360	We quantified the relative occupancy levels of the glycans at each of the five sites and observed complex heterogeneity and site-specific glycosylation.					
31719144	0	32	theme	distinct	35:42	arg1	peculiarities					58:70	distinct site-specific peculiarities	35:70	distinct site-specific peculiarities	35:70	Neutrophil myeloperoxidase harbors distinct site-specific peculiarities in its glycosylation.					
31719144	12	33	theme	paucimannose	1683:1694	arg1	size					1675:1678	the size	1671:1678	the size of paucimannose or smaller	1671:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	3	34	link	neutrophil-derived	358:375	arg1	MPO					339:341	MPO	339:341	MPO	339:341	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	34	link	neutrophil-derived	358:375	arg1	protein					382:388	an abundant neutrophil-derived heme protein	346:388	an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system	346:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	34	link	neutrophil-derived	358:375	arg1	part					398:401	part	398:401	part of the antimicrobial defense system	398:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	12	35	theme	smaller	1699:1705	arg1	size					1675:1678	the size	1671:1678	the size of paucimannose or smaller	1671:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	13	36	located	found	1763:1767	arg2	pattern					1755:1761	the atypical glycosylation pattern	1728:1761	the atypical glycosylation pattern found on MPO	1728:1774	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	13	36	located	found	1763:1767	arg1	MPO					1772:1774	MPO	1772:1774	MPO	1772:1774	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	4	37	from	granules	492:499	arg1	present					465:471	present	465:471	present	465:471	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	37	from	granules	492:499	arg1	protein					444:450	The protein	440:450	The protein	440:450	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	38	theme	azurophilic	480:490	arg1	granules					492:499	the azurophilic granules	476:499	the azurophilic granules of neutrophils	476:514	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	2	39	theme	vasculitides	325:336	arg1	forms					303:307	distinct forms	294:307	distinct forms of small vessel vasculitides	294:336	ANCAs directed to proteinase 3 and myeloperoxidase (MPO) in particular are associated with distinct forms of small vessel vasculitides.					
31719144	8	40	theme	N-glycosylation	968:982	arg1	sites					984:988	5 putative N-glycosylation sites	957:988	5 putative N-glycosylation sites (10 in the mature dimer)	957:1013	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	13	41	theme	specific	1800:1807	arg1	processing					1809:1818	its specific processing	1796:1818	its specific processing	1796:1818	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	7	42	theme	receptor	853:860	arg1	interaction					862:872	receptor interaction	853:872	receptor interaction	853:872	Glycosylation can strongly influence protein function, affecting its folding, receptor interaction, and backbone accessibility.					
31719144	13	43	theme	atypical	1732:1739	arg1	pattern					1755:1761	the atypical glycosylation pattern	1728:1761	the atypical glycosylation pattern found on MPO	1728:1774	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	4	44	attach	present	465:471	arg2	protein					444:450	The protein	440:450	The protein	440:450	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	44	attach	present	465:471	arg1	granules					492:499	the azurophilic granules	476:499	the azurophilic granules of neutrophils	476:514	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	44	attach	present	465:471	arg2	present					465:471	present	465:471	present	465:471	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	12	45	theme	small	1650:1654	arg1	glycans					1656:1662	severely truncated small glycans	1631:1662	severely truncated small glycans	1631:1662	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	7	46	theme	backbone	879:886	arg1	accessibility					888:900	backbone accessibility	879:900	backbone accessibility	879:900	Glycosylation can strongly influence protein function, affecting its folding, receptor interaction, and backbone accessibility.					
31719144	4	47	located	present	465:471	arg2	protein					444:450	The protein	440:450	The protein	440:450	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	47	located	present	465:471	arg1	granules					492:499	the azurophilic granules	476:499	the azurophilic granules of neutrophils	476:514	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	4	47	located	present	465:471	arg2	present					465:471	present	465:471	present	465:471	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	0	48	theme	site-specific	44:56	arg1	peculiarities					58:70	distinct site-specific peculiarities	35:70	distinct site-specific peculiarities	35:70	Neutrophil myeloperoxidase harbors distinct site-specific peculiarities in its glycosylation.					
31719144	10	49	theme	native	1205:1210	arg1	MS					1212:1213	native MS	1205:1213	native MS	1205:1213	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	9	50	theme	MPO	1050:1052	arg1	structure					1054:1062	MPO structure	1050:1062	MPO structure	1050:1062	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	12	51	theme	high	1573:1576	arg1	abundance					1578:1586	a high abundance	1571:1586	a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller	1571:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	12	52	contain	having	1664:1669	arg2	size					1675:1678	the size	1671:1678	the size of paucimannose or smaller	1671:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	12	52	contain	having	1664:1669	arg1	species					1619:1625	phosphorylated high-mannose species	1591:1625	phosphorylated high-mannose species	1591:1625	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	10	53	theme	bottom-up	1175:1183	arg1	glycoproteomics					1185:1199	bottom-up glycoproteomics	1175:1199	bottom-up glycoproteomics	1175:1199	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	3	54	theme	system	432:437	arg1	MPO					339:341	MPO	339:341	MPO	339:341	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	54	theme	system	432:437	arg1	protein					382:388	an abundant neutrophil-derived heme protein	346:388	an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system	346:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	54	theme	system	432:437	arg1	part					398:401	part	398:401	part of the antimicrobial defense system	398:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	12	55	theme	glycans	1656:1662	arg1	abundance					1578:1586	a high abundance	1571:1586	a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller	1571:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	13	56	theme	glycosylation	1741:1753	arg1	pattern					1755:1761	the atypical glycosylation pattern	1728:1761	the atypical glycosylation pattern found on MPO	1728:1774	We hypothesize that the atypical glycosylation pattern found on MPO might contribute to its specific processing and presentation as a self-antigen by antigen-presenting cells.					
31719144	10	57	theme	glycoproteomics	1185:1199	arg1	approaches					1215:1224	bottom-up glycoproteomics and native MS approaches	1175:1224	bottom-up glycoproteomics and native MS approaches	1175:1224	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	4	58	from	present	465:471	arg1	granules					492:499	the azurophilic granules	476:499	the azurophilic granules of neutrophils	476:514	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	12	59	theme	extracellular	1542:1554	arg1	space					1556:1560	the extracellular space	1538:1560	the extracellular space	1538:1560	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	10	60	theme	human	1289:1293	arg1	donors					1295:1300	healthy human donors	1281:1300	healthy human donors	1281:1300	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	4	61	theme	extracellular	556:568	arg1	space					570:574	the extracellular space	552:574	the extracellular space	552:574	The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space.					
31719144	2	62	theme	distinct	294:301	arg1	forms					303:307	distinct forms	294:307	distinct forms of small vessel vasculitides	294:336	ANCAs directed to proteinase 3 and myeloperoxidase (MPO) in particular are associated with distinct forms of small vessel vasculitides.					
31719144	10	63	theme	healthy	1281:1287	arg1	donors					1295:1300	healthy human donors	1281:1300	healthy human donors	1281:1300	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	12	64	gly	glycoproteins	1521:1533	arg1	glycoproteins					1521:1533	glycoproteins	1521:1533	glycoproteins	1521:1533	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	6	65	theme	homodimeric	681:691	arg1	MPO					672:674	MPO	672:674	MPO	672:674	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	6	65	theme	homodimeric	681:691	arg1	glycoprotein					693:704	a homodimeric glycoprotein	679:704	a homodimeric glycoprotein	679:704	MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites.					
31719144	7	66	theme	protein	812:818	arg1	function					820:827	protein function	812:827	protein function	812:827	Glycosylation can strongly influence protein function, affecting its folding, receptor interaction, and backbone accessibility.					
31719144	8	67	gly	N-glycosylation	968:982	arg2	sites					984:988	5 putative N-glycosylation sites	957:988	5 putative N-glycosylation sites (10 in the mature dimer)	957:1013	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	8	67	gly	N-glycosylation	968:982	arg2	5					957:957	5	957:957	5	957:957	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	10	68	from	neutrophils	1266:1276	arg1	MPO					1257:1259	MPO	1257:1259	MPO from neutrophils of healthy human donors	1257:1300	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	8	69	theme	mature	1001:1006	arg1	dimer					1008:1012	the mature dimer	997:1012	the mature dimer	997:1012	MPO potentially can be heavily modified as it harbors 5 putative N-glycosylation sites (10 in the mature dimer).					
31719144	1	70	theme	lysosomal	166:174	arg1	components					176:185	lysosomal components	166:185	lysosomal components of neutrophils	166:200	Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils.					
31719144	12	71	theme	species	1619:1625	arg1	abundance					1578:1586	a high abundance	1571:1586	a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller	1571:1705	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	3	72	theme	neutrophil-derived	358:375	arg1	MPO					339:341	MPO	339:341	MPO	339:341	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	72	theme	neutrophil-derived	358:375	arg1	protein					382:388	an abundant neutrophil-derived heme protein	346:388	an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system	346:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	72	theme	neutrophil-derived	358:375	arg1	part					398:401	part	398:401	part of the antimicrobial defense system	398:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	10	73	theme	donors	1295:1300	arg1	MPO					1257:1259	MPO	1257:1259	MPO from neutrophils of healthy human donors	1257:1300	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31719144	9	74	theme	full	1082:1085	arg1	scope					1087:1091	full scope	1082:1091	full scope	1082:1091	Although considered important for MPO structure and function, the full scope and relative abundance of the glycans attached to MPO is unknown.					
31719144	12	75	theme	truncated	1640:1648	arg1	glycans					1656:1662	severely truncated small glycans	1631:1662	severely truncated small glycans	1631:1662	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	3	76	theme	heme	377:380	arg1	MPO					339:341	MPO	339:341	MPO	339:341	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	76	theme	heme	377:380	arg1	protein					382:388	an abundant neutrophil-derived heme protein	346:388	an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system	346:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	3	76	theme	heme	377:380	arg1	part					398:401	part	398:401	part of the antimicrobial defense system	398:437	MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system.					
31719144	11	77	theme	complex	1401:1407	arg1	heterogeneity					1409:1421	complex heterogeneity	1401:1421	complex heterogeneity	1401:1421	We quantified the relative occupancy levels of the glycans at each of the five sites and observed complex heterogeneity and site-specific glycosylation.					
31719144	12	78	theme	uncommon	1508:1515	arg1	phenotypes					1497:1506	glycosylation phenotypes	1483:1506	glycosylation phenotypes uncommon for glycoproteins	1483:1533	In particular, we detected glycosylation phenotypes uncommon for glycoproteins in the extracellular space, such as a high abundance of phosphorylated high-mannose species and severely truncated small glycans having the size of paucimannose or smaller.					
31719144	10	79	theme	MS	1212:1213	arg1	approaches					1215:1224	bottom-up glycoproteomics and native MS approaches	1175:1224	bottom-up glycoproteomics and native MS approaches	1175:1224	Here, combining bottom-up glycoproteomics and native MS approaches, we structurally characterized MPO from neutrophils of healthy human donors.					
31100702	8	0	theme	detergent	946:954	arg1	removal					956:962	detergent removal	946:962	detergent removal	946:962	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	7	1	theme	second	857:862	arg1	approach					864:871	the second approach	853:871	the second approach	853:871	In the second approach, detergent was removed by adsorption onto a polystyrene resin.					
31100702	13	2	gly	glycoproteins	1733:1745	arg1	glycoproteins					1733:1745	all target glycoproteins	1722:1745	all target glycoproteins	1722:1745	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	4	3	theme	high	581:584	arg1	purity					586:591	at high purity	578:591	at high purity	578:591	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	13	4	theme	target	1726:1731	arg1	glycoproteins					1733:1745	all target glycoproteins	1722:1745	all target glycoproteins	1722:1745	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	4	5	theme	lactoferrin	639:649	arg1	digest					622:627	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	6	gly	glycoprotein	609:620	arg1	glycoprotein					609:620	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	0	7	link	N-linked	51:58	arg1	glycans					60:66	pure protein N-linked glycans	38:66	pure protein N-linked glycans	38:66	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	4	8	theme	glycoprotein	609:620	arg1	digest					622:627	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	10	9	from	Recovery	1131:1138	arg1	lactoferrin					1160:1170	lactoferrin	1160:1170	lactoferrin	1160:1170	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	13	10	theme	glycan	1681:1686	arg1	purification					1688:1699	small-scale glycan purification	1669:1699	small-scale glycan purification	1669:1699	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	5	11	theme	separate	699:706	arg1	approaches					708:717	two separate approaches	695:717	two separate approaches	695:717	Detergents were removed from the digest by two separate approaches.					
31100702	1	12	theme	attached	129:136	arg1	glycans					138:144	the attached glycans	125:144	the attached glycans	125:144	Glycoproteins are biologically active proteins of which the attached glycans contribute to their biological functionality.					
31100702	12	13	theme	RNase	1541:1545	arg1	B					1547:1547	RNase B	1541:1547	RNase B	1541:1547	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	13	theme	RNase	1541:1545	arg1	thyroglobulin					1558:1570	porcine thyroglobulin	1550:1570	porcine thyroglobulin	1550:1570	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	13	theme	RNase	1541:1545	arg1	lactoferrin					1579:1589	human lactoferrin	1573:1589	human lactoferrin	1573:1589	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	3	14	theme	Glycan	306:311	arg1	release					313:319	Glycan release	306:319	Glycan release	306:319	Glycan release, typically performed with the PNGase F enzyme, is achieved on denatured proteins in the presence of detergents which are notoriously difficult to be completely removed.					
31100702	14	15	theme	full	1807:1810	arg1	profile					1821:1827	the full N-glycan profile	1803:1827	the full N-glycan profile	1803:1827	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	14	16	theme	N-glycan	1812:1819	arg1	profile					1821:1827	the full N-glycan profile	1803:1827	the full N-glycan profile	1803:1827	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	15	17	theme	large-scale	1920:1930	arg1	isolation					1945:1953	large-scale quantitative isolation	1920:1953	large-scale quantitative isolation of N-glycan structures	1920:1976	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	10	18	theme	acetone	1212:1218	arg1	precipitation					1220:1232	acetone precipitation	1212:1232	acetone precipitation	1212:1232	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	4	19	from	purity	586:591	arg1	at					578:579	at high purity	578:591	at high purity	578:591	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	14	20	theme	removal	1780:1786	arg1	step					1788:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	12	21	theme	biantennary	1474:1484	arg1	structures					1486:1495	biantennary structures	1474:1495	biantennary structures	1474:1495	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	8	22	theme	solid	1016:1020	arg1	SPE					1040:1042	SPE	1040:1042	SPE	1040:1042	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	8	22	theme	solid	1016:1020	arg1	extraction					1028:1037	solid phase extraction	1016:1037	solid phase extraction (SPE) steps	1016:1049	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	3	23	theme	F	358:358	arg1	enzyme					360:365	the PNGase F enzyme	347:365	the PNGase F enzyme	347:365	Glycan release, typically performed with the PNGase F enzyme, is achieved on denatured proteins in the presence of detergents which are notoriously difficult to be completely removed.					
31100702	13	24	theme	acetone	1621:1627	arg1	step					1660:1663	a commonly used step	1644:1663	a commonly used step for small-scale glycan purification	1644:1699	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	13	24	theme	acetone	1621:1627	arg1	precipitation					1629:1641	acetone precipitation	1621:1641	acetone precipitation	1621:1641	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	11	25	theme	structures	1392:1401	arg1	loss					1363:1366	a loss	1361:1366	a loss of shorter oligomannose structures	1361:1401	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	8	26	theme	extraction	1028:1037	arg1	steps					1045:1049	solid phase extraction (SPE) steps	1016:1049	solid phase extraction (SPE) steps	1016:1049	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	7	27	theme	polystyrene	917:927	arg1	resin					929:933	a polystyrene resin	915:933	a polystyrene resin	915:933	In the second approach, detergent was removed by adsorption onto a polystyrene resin.					
31100702	3	28	theme	denatured	383:391	arg1	proteins					393:400	denatured proteins	383:400	denatured proteins	383:400	Glycan release, typically performed with the PNGase F enzyme, is achieved on denatured proteins in the presence of detergents which are notoriously difficult to be completely removed.					
31100702	11	29	theme	shorter	1371:1377	arg1	structures					1392:1401	shorter oligomannose structures	1371:1401	shorter oligomannose structures	1371:1401	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	3	30	theme	detergents	421:430	arg1	presence					409:416	the presence	405:416	the presence of detergents which are notoriously difficult to be completely removed	405:487	Glycan release, typically performed with the PNGase F enzyme, is achieved on denatured proteins in the presence of detergents which are notoriously difficult to be completely removed.					
31100702	0	31	theme	Large-scale	0:10	arg1	isolation					25:33	Large-scale quantitative isolation	0:33	Large-scale quantitative isolation of pure protein N-linked glycans.	0:67	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	14	32	theme	detergent	1770:1778	arg1	step					1788:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	9	33	theme	detergent	1072:1080	arg1	removal					1082:1088	detergent removal	1072:1088	detergent removal	1072:1088	Both approaches for detergent removal yielded a final glycan purity above 85%.					
31100702	4	34	from	N-glycans	548:556	arg1	digest					622:627	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	34	from	N-glycans	548:556	arg1	yield					568:572	a high yield	561:572	a high yield	561:572	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	34	from	N-glycans	548:556	arg1	purity					586:591	at high purity	578:591	at high purity	578:591	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	14	35	theme	polystyrene	1752:1762	arg1	step					1788:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	8	36	theme	steps	1045:1049	arg1	sequence					1004:1011	a sequence	1002:1011	a sequence of solid phase extraction (SPE) steps	1002:1049	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	0	37	theme	pure	38:41	arg1	glycans					60:66	pure protein N-linked glycans	38:66	pure protein N-linked glycans	38:66	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	15	38	theme	N-glycan	1958:1965	arg1	structures					1967:1976	N-glycan structures	1958:1976	N-glycan structures	1958:1976	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	9	39	theme	final	1100:1104	arg1	purity					1113:1118	a final glycan purity	1098:1118	a final glycan purity	1098:1118	Both approaches for detergent removal yielded a final glycan purity above 85%.					
31100702	0	40	theme	N-linked	51:58	arg1	glycans					60:66	pure protein N-linked glycans	38:66	pure protein N-linked glycans	38:66	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	12	41	theme	glycans	1427:1433	arg1	loss					1419:1422	A loss	1417:1422	A loss of glycans of lesser complexity (oligomannose and biantennary structures)	1417:1496	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	6	42	theme	first	727:731	arg1	approach					733:740	the first approach	723:740	the first approach	723:740	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	10	43	theme	glycans	1147:1153	arg1	Recovery					1131:1138	Recovery	1131:1138	Recovery of the glycans from lactoferrin	1131:1170	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	12	44	theme	lesser	1438:1443	arg1	complexity					1445:1454	lesser complexity	1438:1454	lesser complexity (oligomannose and biantennary structures)	1438:1496	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	11	45	theme	acetone	1325:1331	arg1	step					1347:1350	the acetone precipitation step	1321:1350	the acetone precipitation step	1321:1350	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	12	46	dep	glycoproteins	1526:1538	arg1	B					1547:1547	RNase B	1541:1547	RNase B	1541:1547	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	46	dep	glycoproteins	1526:1538	arg1	thyroglobulin					1558:1570	porcine thyroglobulin	1550:1570	porcine thyroglobulin	1550:1570	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	46	dep	glycoproteins	1526:1538	arg1	lactoferrin					1579:1589	human lactoferrin	1573:1589	human lactoferrin	1573:1589	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	4	47	theme	at	578:579	arg1	purity					586:591	at high purity	578:591	at high purity	578:591	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	11	48	theme	step	1347:1350	arg1	analysis					1309:1316	A more detailed analysis	1293:1316	A more detailed analysis of the acetone precipitation step	1293:1350	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	2	49	theme	protein	292:298	arg1	core					300:303	the protein core	288:303	the protein core	288:303	Limited data is available on the functional properties of these N-glycans in isolation, without the protein core.					
31100702	15	50	theme	functional	2018:2027	arg1	studies					2029:2035	functional studies	2018:2035	functional studies	2018:2035	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	4	51	theme	bovine	632:637	arg1	lactoferrin					639:649	bovine lactoferrin	632:649	bovine lactoferrin	632:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	12	52	gly	glycoproteins	1526:1538	arg1	glycoproteins					1526:1538	other glycoproteins	1520:1538	other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin)	1520:1590	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	14	53	theme	mammalian	1857:1865	arg1	glycoproteins					1867:1879	all mammalian glycoproteins	1853:1879	all mammalian glycoproteins tested	1853:1886	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	12	54	theme	porcine	1550:1556	arg1	B					1547:1547	RNase B	1541:1547	RNase B	1541:1547	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	54	theme	porcine	1550:1556	arg1	thyroglobulin					1558:1570	porcine thyroglobulin	1550:1570	porcine thyroglobulin	1550:1570	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	15	55	theme	quantitative	1932:1943	arg1	isolation					1945:1953	large-scale quantitative isolation	1920:1953	large-scale quantitative isolation of N-glycan structures	1920:1976	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	4	56	from	digest	622:627	arg1	yield					568:572	a high yield	561:572	a high yield	561:572	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	56	from	digest	622:627	arg1	purity					586:591	at high purity	578:591	at high purity	578:591	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	56	from	digest	622:627	arg1	N-glycans					548:556	N-glycans	548:556	N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin	548:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	4	57	theme	F	607:607	arg1	digest					622:627	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	13	58	theme	small-scale	1669:1679	arg1	purification					1688:1699	small-scale glycan purification	1669:1699	small-scale glycan purification	1669:1699	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	2	59	theme	N-glycans	256:264	arg1	properties					236:245	the functional properties	221:245	the functional properties of these N-glycans in isolation	221:277	Limited data is available on the functional properties of these N-glycans in isolation, without the protein core.					
31100702	6	60	with	precipitated	768:779	arg1	acetone					786:792	acetone	786:792	acetone	786:792	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	10	61	from	lactoferrin	1160:1170	arg1	Recovery					1131:1138	Recovery	1131:1138	Recovery of the glycans from lactoferrin	1131:1170	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	10	61	from	lactoferrin	1160:1170	arg1	glycans					1147:1153	the glycans	1143:1153	the glycans from lactoferrin	1143:1170	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	14	62	gly	glycoproteins	1867:1879	arg1	glycoproteins					1867:1879	all mammalian glycoproteins	1853:1879	all mammalian glycoproteins tested	1853:1886	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	6	63	theme	containing	812:821	arg1	supernatant					823:833	the detergent containing supernatant	798:833	the detergent containing supernatant	798:833	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	12	64	theme	other	1520:1524	arg1	glycoproteins					1526:1538	other glycoproteins	1520:1538	other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin)	1520:1590	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	3	65	theme	PNGase	351:356	arg1	enzyme					360:365	the PNGase F enzyme	347:365	the PNGase F enzyme	347:365	Glycan release, typically performed with the PNGase F enzyme, is achieved on denatured proteins in the presence of detergents which are notoriously difficult to be completely removed.					
31100702	1	66	theme	biological	166:175	arg1	functionality					177:189	their biological functionality	160:189	their biological functionality	160:189	Glycoproteins are biologically active proteins of which the attached glycans contribute to their biological functionality.					
31100702	13	67	theme	used	1655:1658	arg1	step					1660:1663	a commonly used step	1644:1663	a commonly used step for small-scale glycan purification	1644:1699	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	13	67	theme	used	1655:1658	arg1	precipitation					1629:1641	acetone precipitation	1621:1641	acetone precipitation	1621:1641	These results indicate that acetone precipitation, a commonly used step for small-scale glycan purification, is not suitable for all target glycoproteins.					
31100702	8	68	theme	phase	1022:1026	arg1	SPE					1040:1042	SPE	1040:1042	SPE	1040:1042	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	8	68	theme	phase	1022:1026	arg1	extraction					1028:1037	solid phase extraction	1016:1037	solid phase extraction (SPE) steps	1016:1049	Following detergent removal, the glycans were further purified by a sequence of solid phase extraction (SPE) steps.					
31100702	10	69	theme	resin	1257:1261	arg1	%					1266:1266	the polystyrene resin; 52%	1241:1266	the polystyrene resin; 52%	1241:1266	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	12	70	dep	complexity	1445:1454	arg1	oligomannose					1457:1468	oligomannose	1457:1468	oligomannose	1457:1468	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	70	dep	complexity	1445:1454	arg1	structures					1486:1495	biantennary structures	1474:1495	biantennary structures	1474:1495	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	11	71	theme	oligomannose	1379:1390	arg1	structures					1392:1401	shorter oligomannose structures	1371:1401	shorter oligomannose structures	1371:1401	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	2	72	from	properties	236:245	arg1	isolation					269:277	isolation	269:277	isolation	269:277	Limited data is available on the functional properties of these N-glycans in isolation, without the protein core.					
31100702	11	73	theme	detailed	1300:1307	arg1	analysis					1309:1316	A more detailed analysis	1293:1316	A more detailed analysis of the acetone precipitation step	1293:1350	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31100702	0	74	theme	quantitative	12:23	arg1	isolation					25:33	Large-scale quantitative isolation	0:33	Large-scale quantitative isolation of pure protein N-linked glycans.	0:67	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	1	75	theme	active	100:105	arg1	proteins					107:114	biologically active proteins	87:114	biologically active proteins of which the attached glycans contribute to their biological functionality	87:189	Glycoproteins are biologically active proteins of which the attached glycans contribute to their biological functionality.					
31100702	1	75	theme	active	100:105	arg1	Glycoproteins					69:81	Glycoproteins	69:81	Glycoproteins	69:81	Glycoproteins are biologically active proteins of which the attached glycans contribute to their biological functionality.					
31100702	5	76	attach	removed	668:674	arg2	Detergents					652:661	Detergents	652:661	Detergents	652:661	Detergents were removed from the digest by two separate approaches.					
31100702	5	76	attach	removed	668:674	arg1	digest					685:690	the digest	681:690	the digest	681:690	Detergents were removed from the digest by two separate approaches.					
31100702	14	77	theme	resin	1764:1768	arg1	step					1788:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step	1748:1791	The polystyrene resin detergent removal step conserved the full N-glycan profile and could be applied to all mammalian glycoproteins tested.					
31100702	12	78	theme	human	1573:1577	arg1	B					1547:1547	RNase B	1541:1547	RNase B	1541:1547	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	12	78	theme	human	1573:1577	arg1	lactoferrin					1579:1589	human lactoferrin	1573:1589	human lactoferrin	1573:1589	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	4	79	theme	high	563:566	arg1	yield					568:572	a high yield	561:572	a high yield	561:572	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	15	80	theme	sufficient	1996:2005	arg1	purity					2007:2012	sufficient purity	1996:2012	sufficient purity for functional studies	1996:2035	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	0	81	theme	protein	43:49	arg1	glycans					60:66	pure protein N-linked glycans	38:66	pure protein N-linked glycans	38:66	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	10	82	theme	polystyrene	1245:1255	arg1	%					1266:1266	the polystyrene resin; 52%	1241:1266	the polystyrene resin; 52%	1241:1266	Recovery of the glycans from lactoferrin was, however, much lower when utilizing acetone precipitation versus the polystyrene resin; 52% versus 85% respectively.					
31100702	12	83	theme	complexity	1445:1454	arg1	glycans					1427:1433	glycans	1427:1433	glycans of lesser complexity (oligomannose and biantennary structures)	1427:1496	A loss of glycans of lesser complexity (oligomannose and biantennary structures) was also observed for other glycoproteins (RNase B, porcine thyroglobulin, human lactoferrin).					
31100702	6	84	from	precipitated	768:779	arg1	approach					733:740	the first approach	723:740	the first approach	723:740	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	2	85	theme	Limited	192:198	arg1	data					200:203	Limited data	192:203	Limited data	192:203	Limited data is available on the functional properties of these N-glycans in isolation, without the protein core.					
31100702	0	86	theme	glycans	60:66	arg1	isolation					25:33	Large-scale quantitative isolation	0:33	Large-scale quantitative isolation of pure protein N-linked glycans.	0:67	Large-scale quantitative isolation of pure protein N-linked glycans.					
31100702	6	87	from	approach	733:740	arg1	precipitated					768:779	precipitated	768:779	precipitated	768:779	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	15	88	theme	structures	1967:1976	arg1	isolation					1945:1953	large-scale quantitative isolation	1920:1953	large-scale quantitative isolation of N-glycan structures	1920:1976	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	2	89	theme	functional	225:234	arg1	properties					236:245	the functional properties	221:245	the functional properties of these N-glycans in isolation	221:277	Limited data is available on the functional properties of these N-glycans in isolation, without the protein core.					
31100702	15	90	theme	optimized	1900:1908	arg1	protocol					1910:1917	this optimized protocol	1895:1917	this optimized protocol	1895:1917	Using this optimized protocol, large-scale quantitative isolation of N-glycan structures was achieved with sufficient purity for functional studies.					
31100702	9	91	theme	glycan	1106:1111	arg1	purity					1113:1118	a final glycan purity	1098:1118	a final glycan purity	1098:1118	Both approaches for detergent removal yielded a final glycan purity above 85%.					
31100702	6	92	theme	detergent	802:810	arg1	supernatant					823:833	the detergent containing supernatant	798:833	the detergent containing supernatant	798:833	In the first approach, protein and glycans were precipitated with acetone and the detergent containing supernatant was discarded.					
31100702	4	93	theme	PNGase	600:605	arg1	digest					622:627	a PNGase F glycoprotein digest	598:627	a PNGase F glycoprotein digest of bovine lactoferrin	598:649	In this work we compared two methods aiming at recovering N-glycans in a high yield and at high purity from a PNGase F glycoprotein digest of bovine lactoferrin.					
31100702	11	94	theme	precipitation	1333:1345	arg1	step					1347:1350	the acetone precipitation step	1321:1350	the acetone precipitation step	1321:1350	A more detailed analysis of the acetone precipitation step revealed a loss of shorter oligomannose structures specifically.					
31882545	4	0	gly	glycosylation	618:630	arg1	factor					686:691	the phosphate-regulating hormone fibroblast growth factor 23	635:694	the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	635:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	0	gly	glycosylation	618:630	arg1	FGF23					697:701	FGF23	697:701	FGF23	697:701	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	3	1	theme	hyperphosphatemic	489:505	arg1	HFTC					536:539	HFTC	536:539	HFTC	536:539	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	1	theme	hyperphosphatemic	489:505	arg1	calcinosis					524:533	the disease hyperphosphatemic familial tumoral calcinosis	477:533	the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	477:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	4	2	theme	GALNT3	560:565	arg1	activity					567:574	GALNT3 activity	560:574	GALNT3 activity	560:574	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	3	3	dep	UDP-GalNAc	366:375	arg1	N-acetylgalactosaminyltransferase					389:421	polypeptide N-acetylgalactosaminyltransferase 3	377:423	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	366:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	3	dep	UDP-GalNAc	366:375	arg1	Galnt3					426:431	Galnt3	426:431	Galnt3	426:431	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	4	theme	familial	507:514	arg1	HFTC					536:539	HFTC	536:539	HFTC	536:539	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	4	theme	familial	507:514	arg1	calcinosis					524:533	the disease hyperphosphatemic familial tumoral calcinosis	477:533	the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	477:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	9	5	dep	composition	1483:1493	arg1	the					1479:1481	the	1479:1481	the	1479:1481	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	1	6	from	health	165:170	arg1	disease					194:200	disease	194:200	disease	194:200	The importance of the microbiome in health and its disruption in disease is continuing to be elucidated.					
31882545	2	7	theme	factors	283:289	arg1	unknown					339:345	unknown	339:345	unknown	339:345	However, the multitude of host and environmental factors that influence the microbiome are still largely unknown.					
31882545	2	7	theme	factors	283:289	arg1	multitude					247:255	the multitude	243:255	the multitude of host and environmental factors that influence the microbiome	243:319	However, the multitude of host and environmental factors that influence the microbiome are still largely unknown.					
31882545	7	8	from	changes	1131:1137	arg1	composition					1157:1167	composition	1157:1167	composition	1157:1167	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	7	8	from	changes	1131:1137	arg1	stability					1174:1182	stability	1174:1182	stability	1174:1182	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	7	8	from	changes	1131:1137	arg1	structure					1146:1154	structure	1146:1154	structure	1146:1154	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	8	9	theme	in	1280:1281	arg1	substrate					1288:1296	an in vivo substrate	1277:1296	an in vivo substrate of Galnt3	1277:1306	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	9	theme	in	1280:1281	arg1	mucin					1260:1264	the major secreted salivary mucin	1232:1264	the major secreted salivary mucin	1232:1264	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	10	10	theme	oral	1550:1553	arg1	findings					1555:1562	oral findings	1550:1562	oral findings	1550:1562	Considering that oral findings have been documented in HFTC patients, our study suggests that investigating GALNT3-mediated changes in the oral microbiome may be warranted.					
31882545	2	11	theme	environmental	269:281	arg1	factors					283:289	environmental factors	269:289	environmental factors	269:289	However, the multitude of host and environmental factors that influence the microbiome are still largely unknown.					
31882545	4	12	theme	activity	567:574	arg1	loss					552:555	loss	552:555	loss of GALNT3 activity in the bone	552:586	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	0	13	theme	microbiome	117:126	arg1	glycosylation					71:83	Muc10 glycosylation	65:83	Muc10 glycosylation	65:83	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	0	13	theme	microbiome	117:126	arg1	composition					93:103	the composition	89:103	the composition of the oral microbiome	89:126	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	9	14	gly	glycosylation	1442:1454	arg1	Muc10					1459:1463	Muc10	1459:1463	Muc10	1459:1463	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	3	15	dep	N-acetylgalactosaminyltransferase	389:421	arg1	mice					444:447	-deficient mice	433:447	-deficient mice	433:447	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	15	dep	N-acetylgalactosaminyltransferase	389:421	arg1	model					467:471	a model	465:471	a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	465:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	8	16	theme	secreted	1242:1249	arg1	mucin					1260:1264	the major secreted salivary mucin	1232:1264	the major secreted salivary mucin	1232:1264	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	16	theme	secreted	1242:1249	arg1	substrate					1288:1296	an in vivo substrate	1277:1296	an in vivo substrate of Galnt3	1277:1306	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	16	theme	secreted	1242:1249	arg1	Muc10					1267:1271	Muc10	1267:1271	Muc10	1267:1271	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	10	17	theme	HFTC	1588:1591	arg1	patients					1593:1600	HFTC patients	1588:1600	HFTC patients	1588:1600	Considering that oral findings have been documented in HFTC patients, our study suggests that investigating GALNT3-mediated changes in the oral microbiome may be warranted.					
31882545	0	18	theme	oral	112:115	arg1	microbiome					117:126	the oral microbiome	108:126	the oral microbiome	108:126	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	6	19	theme	major	966:970	arg1	Galnt3					952:957	Galnt3	952:957	Galnt3	952:957	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	6	19	theme	major	966:970	arg1	O-glycosyltransferase					972:992	the major O-glycosyltransferase	962:992	the major O-glycosyltransferase expressed in the secretory cells of salivary glands	962:1044	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	1	20	from	importance	133:142	arg1	disruption					180:189	its disruption	176:189	its disruption in disease	176:200	The importance of the microbiome in health and its disruption in disease is continuing to be elucidated.					
31882545	1	20	from	importance	133:142	arg1	health					165:170	health	165:170	health	165:170	The importance of the microbiome in health and its disruption in disease is continuing to be elucidated.					
31882545	2	21	theme	host	260:263	arg1	unknown					339:345	unknown	339:345	unknown	339:345	However, the multitude of host and environmental factors that influence the microbiome are still largely unknown.					
31882545	2	21	theme	host	260:263	arg1	multitude					247:255	the multitude	243:255	the multitude of host and environmental factors that influence the microbiome	243:319	However, the multitude of host and environmental factors that influence the microbiome are still largely unknown.					
31882545	9	22	theme	various	1374:1380	arg1	microbes					1382:1389	various microbes	1374:1389	various microbes	1374:1389	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	4	23	from	loss	552:555	arg1	bone					583:586	the bone	579:586	the bone	579:586	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	7	24	theme	16S	1061:1063	arg1	sequencing					1075:1084	16S rRNA gene sequencing	1061:1084	16S rRNA gene sequencing	1061:1084	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	10	25	from	changes	1657:1663	arg1	microbiome					1677:1686	the oral microbiome	1668:1686	the oral microbiome	1668:1686	Considering that oral findings have been documented in HFTC patients, our study suggests that investigating GALNT3-mediated changes in the oral microbiome may be warranted.					
31882545	9	26	theme	Muc10	1459:1463	arg1	glycosylation					1442:1454	glycosylation	1442:1454	glycosylation of Muc10, which alters the composition and stability of the oral microbiome	1442:1530	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	4	27	theme	phosphate-regulating	639:658	arg1	factor					686:691	the phosphate-regulating hormone fibroblast growth factor 23	635:694	the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	635:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	27	theme	phosphate-regulating	639:658	arg1	FGF23					697:701	FGF23	697:701	FGF23	697:701	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	3	28	theme	polypeptide	377:387	arg1	N-acetylgalactosaminyltransferase					389:421	polypeptide N-acetylgalactosaminyltransferase 3	377:423	UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	366:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	28	theme	polypeptide	377:387	arg1	Galnt3					426:431	Galnt3	426:431	Galnt3	426:431	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	7	29	theme	rRNA	1065:1068	arg1	sequencing					1075:1084	16S rRNA gene sequencing	1061:1084	16S rRNA gene sequencing	1061:1084	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	7	30	theme	gene	1070:1073	arg1	sequencing					1075:1084	16S rRNA gene sequencing	1061:1084	16S rRNA gene sequencing	1061:1084	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	4	31	theme	subdermal	740:748	arg1	tumors					760:765	subdermal calcified tumors	740:765	subdermal calcified tumors	740:765	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	3	32	theme	-deficient	433:442	arg1	mice					444:447	-deficient mice	433:447	-deficient mice	433:447	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	32	theme	-deficient	433:442	arg1	model					467:471	a model	465:471	a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	465:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	0	33	theme	disease-associated	12:29	arg1	Galnt3					51:56	the disease-associated glycosyltransferase Galnt3	8:56	the disease-associated glycosyltransferase Galnt3	8:56	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	4	34	theme	factor	686:691	arg1	glycosylation					618:630	altered glycosylation	610:630	altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	610:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	6	35	theme	salivary	1030:1037	arg1	glands					1039:1044	salivary glands	1030:1044	salivary glands	1030:1044	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	5	36	theme	other	800:804	arg1	tissues					806:812	other tissues	800:812	other tissues	800:812	However, GALNT3 is expressed in other tissues in addition to bone, suggesting that systemic loss could result in other pathologies.					
31882545	10	37	theme	oral	1672:1675	arg1	microbiome					1677:1686	the oral microbiome	1668:1686	the oral microbiome	1668:1686	Considering that oral findings have been documented in HFTC patients, our study suggests that investigating GALNT3-mediated changes in the oral microbiome may be warranted.					
31882545	4	38	theme	growth	679:684	arg1	factor					686:691	the phosphate-regulating hormone fibroblast growth factor 23	635:694	the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	635:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	38	theme	growth	679:684	arg1	FGF23					697:701	FGF23	697:701	FGF23	697:701	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	39	theme	fibroblast	668:677	arg1	factor					686:691	the phosphate-regulating hormone fibroblast growth factor 23	635:694	the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	635:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	39	theme	fibroblast	668:677	arg1	FGF23					697:701	FGF23	697:701	FGF23	697:701	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	0	40	theme	Galnt3	51:56	arg1	Loss					0:3	Loss	0:3	Loss of the disease-associated glycosyltransferase Galnt3	0:56	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	5	41	theme	other	881:885	arg1	pathologies					887:897	other pathologies	881:897	other pathologies	881:897	However, GALNT3 is expressed in other tissues in addition to bone, suggesting that systemic loss could result in other pathologies.					
31882545	8	42	theme	major	1236:1240	arg1	mucin					1260:1264	the major secreted salivary mucin	1232:1264	the major secreted salivary mucin	1232:1264	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	42	theme	major	1236:1240	arg1	substrate					1288:1296	an in vivo substrate	1277:1296	an in vivo substrate of Galnt3	1277:1306	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	42	theme	major	1236:1240	arg1	Muc10					1267:1271	Muc10	1267:1271	Muc10	1267:1271	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	9	43	theme	oral	1516:1519	arg1	microbiome					1521:1530	the oral microbiome	1512:1530	the oral microbiome	1512:1530	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	6	44	theme	glands	1039:1044	arg1	cells					1021:1025	the secretory cells	1007:1025	the secretory cells of salivary glands	1007:1044	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	4	45	theme	altered	610:616	arg1	glycosylation					618:630	altered glycosylation	610:630	altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	610:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	0	46	theme	glycosyltransferase	31:49	arg1	Galnt3					51:56	the disease-associated glycosyltransferase Galnt3	8:56	the disease-associated glycosyltransferase Galnt3	8:56	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	9	47	theme	microbiome	1521:1530	arg1	stability					1499:1507	stability	1499:1507	stability	1499:1507	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	9	47	theme	microbiome	1521:1530	arg1	composition					1483:1493	composition	1483:1493	composition	1483:1493	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	6	48	theme	secretory	1011:1019	arg1	cells					1021:1025	the secretory cells	1007:1025	the secretory cells of salivary glands	1007:1044	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	0	49	theme	Muc10	65:69	arg1	glycosylation					71:83	Muc10 glycosylation	65:83	Muc10 glycosylation	65:83	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	5	50	theme	systemic	851:858	arg1	loss					860:863	systemic loss	851:863	systemic loss	851:863	However, GALNT3 is expressed in other tissues in addition to bone, suggesting that systemic loss could result in other pathologies.					
31882545	10	51	theme	GALNT3-mediated	1641:1655	arg1	changes					1657:1663	GALNT3-mediated changes	1641:1663	GALNT3-mediated changes in the oral microbiome	1641:1686	Considering that oral findings have been documented in HFTC patients, our study suggests that investigating GALNT3-mediated changes in the oral microbiome may be warranted.					
31882545	8	52	theme	Galnt3	1301:1306	arg1	substrate					1288:1296	an in vivo substrate	1277:1296	an in vivo substrate of Galnt3	1277:1306	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	52	theme	Galnt3	1301:1306	arg1	mucin					1260:1264	the major secreted salivary mucin	1232:1264	the major secreted salivary mucin	1232:1264	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	53	theme	salivary	1251:1258	arg1	mucin					1260:1264	the major secreted salivary mucin	1232:1264	the major secreted salivary mucin	1232:1264	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	53	theme	salivary	1251:1258	arg1	substrate					1288:1296	an in vivo substrate	1277:1296	an in vivo substrate of Galnt3	1277:1306	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	8	53	theme	salivary	1251:1258	arg1	Muc10					1267:1271	Muc10	1267:1271	Muc10	1267:1271	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	7	54	theme	Galnt3	1112:1117	arg1	loss					1104:1107	the loss	1100:1107	the loss of Galnt3	1100:1117	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	6	55	theme	semiquantitative	906:921	arg1	PCR					933:935	semiquantitative real-time PCR	906:935	semiquantitative real-time PCR	906:935	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31882545	3	56	theme	disease	481:487	arg1	HFTC					536:539	HFTC	536:539	HFTC	536:539	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	56	theme	disease	481:487	arg1	calcinosis					524:533	the disease hyperphosphatemic familial tumoral calcinosis	477:533	the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	477:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	9	57	theme	Galnt3	1425:1430	arg1	loss					1417:1420	loss	1417:1420	loss of Galnt3	1417:1430	Given that mucins and their O-glycans are known to interact with various microbes, our results suggest that loss of Galnt3 decreases glycosylation of Muc10, which alters the composition and stability of the oral microbiome.					
31882545	4	58	theme	calcified	750:758	arg1	tumors					760:765	subdermal calcified tumors	740:765	subdermal calcified tumors	740:765	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	0	59	gly	glycosylation	71:83	arg1	microbiome					117:126	the oral microbiome	108:126	the oral microbiome	108:126	Loss of the disease-associated glycosyltransferase Galnt3 alters Muc10 glycosylation and the composition of the oral microbiome.					
31882545	1	60	from	disruption	180:189	arg1	disease					194:200	disease	194:200	disease	194:200	The importance of the microbiome in health and its disruption in disease is continuing to be elucidated.					
31882545	4	61	theme	hormone	660:666	arg1	factor					686:691	the phosphate-regulating hormone fibroblast growth factor 23	635:694	the phosphate-regulating hormone fibroblast growth factor 23 (FGF23)	635:702	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	4	61	theme	hormone	660:666	arg1	FGF23					697:701	FGF23	697:701	FGF23	697:701	In HFTC, loss of GALNT3 activity in the bone is thought to lead to altered glycosylation of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23), resulting in hyperphosphatemia and subdermal calcified tumors.					
31882545	8	62	dep	in	1280:1281	arg1	vivo					1283:1286	vivo	1283:1286	vivo	1283:1286	Moreover, we identified the major secreted salivary mucin, Muc10, as an in vivo substrate of Galnt3.					
31882545	3	63	theme	tumoral	516:522	arg1	HFTC					536:539	HFTC	536:539	HFTC	536:539	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	3	63	theme	tumoral	516:522	arg1	calcinosis					524:533	the disease hyperphosphatemic familial tumoral calcinosis	477:533	the disease hyperphosphatemic familial tumoral calcinosis (HFTC)	477:540	Here, we examined UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 (Galnt3)-deficient mice, which serve as a model for the disease hyperphosphatemic familial tumoral calcinosis (HFTC).					
31882545	7	64	theme	oral	1191:1194	arg1	microbiome					1196:1205	the oral microbiome	1187:1205	the oral microbiome	1187:1205	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	1	65	theme	microbiome	151:160	arg1	importance					133:142	The importance	129:142	The importance of the microbiome in health and its disruption in disease	129:200	The importance of the microbiome in health and its disruption in disease is continuing to be elucidated.					
31882545	7	66	theme	microbiome	1196:1205	arg1	composition					1157:1167	composition	1157:1167	composition	1157:1167	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	7	66	theme	microbiome	1196:1205	arg1	stability					1174:1182	stability	1174:1182	stability	1174:1182	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	7	66	theme	microbiome	1196:1205	arg1	structure					1146:1154	structure	1146:1154	structure	1146:1154	Additionally, 16S rRNA gene sequencing revealed that the loss of Galnt3 resulted in changes in the structure, composition, and stability of the oral microbiome.					
31882545	6	67	theme	real-time	923:931	arg1	PCR					933:935	semiquantitative real-time PCR	906:935	semiquantitative real-time PCR	906:935	Using semiquantitative real-time PCR, we found that Galnt3 is the major O-glycosyltransferase expressed in the secretory cells of salivary glands.					
31370181	3	0	theme	mg/L	647:650	arg1	concentration					627:639	a concentration	625:639	a concentration of 20 mg/L	625:650	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	7	1	theme	glycosylation	1384:1396	arg1	profile					1398:1404	the glycosylation profile	1380:1404	the glycosylation profile	1380:1404	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	2	2	theme	glycoprotein	358:369	arg1	gB					374:375	gB	374:375	gB	374:375	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	2	theme	glycoprotein	358:369	arg1	B					371:371	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	6	3	theme	CHO-produced	1234:1245	arg1	gB					1247:1248	CHO-produced gB	1234:1248	CHO-produced gB	1234:1248	The profiles were strikingly comparable between BY-2- and CHO-produced gB.					
31370181	2	4	theme	suspension	456:465	arg1	cells					467:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	5	5	theme	type	1013:1016	arg1	N-glycans					1018:1026	oligomannosidic type N-glycans	997:1026	oligomannosidic type N-glycans	997:1026	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	1	6	gly	glycosylation	161:173	arg1	glycoproteins					204:216	recombinant viral glycoproteins	186:216	recombinant viral glycoproteins	186:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	4	7	theme	multienzymatic	855:868	arg1	proteolysis					870:880	multienzymatic proteolysis	855:880	multienzymatic proteolysis	855:880	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	5	8	theme	additional	1089:1098	arg1	structures					1108:1117	additional Lewis A structures	1089:1117	additional Lewis A structures (BY-2)	1089:1124	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	8	theme	additional	1089:1098	arg1	BY-2					1120:1123	BY-2	1120:1123	BY-2	1120:1123	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	0	9	theme	CHO	96:98	arg1	Cells					127:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	8	10	theme	suspension	1456:1465	arg1	system					1505:1510	an alternative expression system	1479:1510	an alternative expression system	1479:1510	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	8	10	theme	suspension	1456:1465	arg1	cultures					1467:1474	the BY-2 suspension cultures	1447:1474	the BY-2 suspension cultures	1447:1474	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	2	11	theme	ovary	560:564	arg1	cells					572:576	Chinese hamster ovary (CHO) cells	544:576	Chinese hamster ovary (CHO) cells	544:576	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	5	12	with	cases	1078:1082	arg1	structures					1108:1117	additional Lewis A structures	1089:1117	additional Lewis A structures (BY-2)	1089:1124	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	12	with	cases	1078:1082	arg1	CHO					1170:1172	CHO	1170:1172	CHO	1170:1172	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	12	with	cases	1078:1082	arg1	beta-1,4-galactose					1134:1151	beta-1,4-galactose	1134:1151	beta-1,4-galactose	1134:1151	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	12	with	cases	1078:1082	arg1	acid					1164:1167	sialic acid	1157:1167	sialic acid (CHO)	1157:1173	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	12	with	cases	1078:1082	arg1	BY-2					1120:1123	BY-2	1120:1123	BY-2	1120:1123	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	13	dep	differed	931:938	arg1	occupied					973:980	occupied	973:980	being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO)	967:1173	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	0	14	theme	tabacum	114:120	arg1	Cells					127:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	8	15	theme	BY-2	1451:1454	arg1	system					1505:1510	an alternative expression system	1479:1510	an alternative expression system	1479:1510	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	8	15	theme	BY-2	1451:1454	arg1	cultures					1467:1474	the BY-2 suspension cultures	1447:1474	the BY-2 suspension cultures	1447:1474	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	1	16	gly	glycoproteins	204:216	arg1	glycoproteins					204:216	recombinant viral glycoproteins	186:216	recombinant viral glycoproteins	186:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	1	17	theme	glycosylation	161:173	arg1	pattern					175:181	the glycosylation pattern	157:181	the glycosylation pattern of recombinant viral glycoproteins	157:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	0	18	theme	Nicotiana	104:112	arg1	tabacum					114:120	Nicotiana tabacum	104:120	Nicotiana tabacum	104:120	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	7	19	dep	suggest	1265:1271	arg1	influences					1369:1378	influences	1369:1378	suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site	1265:1417	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	4	20	theme	present	796:802	arg1	N-glycans					786:794	N-glycans present	786:802	N-glycans present	786:802	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	7	21	theme	site	1350:1353	arg1	glycoproteins					1304:1316	glycoproteins	1304:1316	glycoproteins	1304:1316	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	7	21	theme	site	1350:1353	arg1	accessibility					1355:1367	site accessibility	1350:1367	site accessibility	1350:1367	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	3	22	theme	size	712:715	arg1	chromatography					727:740	size exclusion chromatography	712:740	size exclusion chromatography	712:740	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	2	23	theme	Bright	433:438	arg1	cells					467:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	5	24	theme	complex	1042:1048	arg1	N-glycans					1050:1058	complex N-glycans	1042:1058	complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO)	1042:1173	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	8	25	theme	alternative	1482:1492	arg1	system					1505:1510	an alternative expression system	1479:1510	an alternative expression system	1479:1510	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	8	25	theme	alternative	1482:1492	arg1	cultures					1467:1474	the BY-2 suspension cultures	1447:1474	the BY-2 suspension cultures	1447:1474	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	7	26	theme	plant	1335:1339	arg1	cells					1341:1345	plant cells	1335:1345	plant cells	1335:1345	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	2	27	theme	tabacum	425:431	arg1	cells					467:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	5	28	theme	A	1106:1106	arg1	structures					1108:1117	additional Lewis A structures	1089:1117	additional Lewis A structures (BY-2)	1089:1124	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	28	theme	A	1106:1106	arg1	BY-2					1120:1123	BY-2	1120:1123	BY-2	1120:1123	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	0	29	theme	B	54:54	arg1	Profile					30:36	the N-Glycosylation Profile	10:36	the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells	10:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	1	30	theme	recombinant	186:196	arg1	glycoproteins					204:216	recombinant viral glycoproteins	186:216	recombinant viral glycoproteins	186:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	2	31	theme	Nicotiana	415:423	arg1	tabacum					425:431	Nicotiana tabacum	415:431	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	5	32	used	occupied	973:980	arg2	sites					961:965	some sites	956:965	some sites	956:965	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	1	33	theme	viral	198:202	arg1	glycoproteins					204:216	recombinant viral glycoproteins	186:216	recombinant viral glycoproteins	186:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	0	34	theme	N-Glycosylation	14:28	arg1	Profile					30:36	the N-Glycosylation Profile	10:36	the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells	10:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	3	35	theme	ammonium	677:684	arg1	precipitation					694:706	ammonium sulfate precipitation	677:706	ammonium sulfate precipitation	677:706	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	0	36	theme	BY-2	122:125	arg1	Cells					127:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	CHO and Nicotiana tabacum BY-2 Cells	96:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	2	37	theme	study	296:300	arg1	aim					284:286	The aim	280:286	The aim of this study	280:300	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	1	38	theme	glycoproteins	204:216	arg1	pattern					175:181	the glycosylation pattern	157:181	the glycosylation pattern of recombinant viral glycoproteins	157:216	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	0	39	theme	Glycoprotein	41:52	arg1	B					54:54	Glycoprotein B	41:54	Glycoprotein B	41:54	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	3	40	theme	sulfate	686:692	arg1	precipitation					694:706	ammonium sulfate precipitation	677:706	ammonium sulfate precipitation	677:706	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	3	41	theme	BY-2	602:605	arg1	medium					615:620	the BY-2 culture medium	598:620	the BY-2 culture medium	598:620	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	2	42	theme	CHO	567:569	arg1	cells					572:576	Chinese hamster ovary (CHO) cells	544:576	Chinese hamster ovary (CHO) cells	544:576	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	43	theme	BY-2	450:453	arg1	cells					467:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	44	gly	glycoprotein	358:369	arg1	glycoprotein					358:369	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	0	45	from	Cytomegalovirus	67:81	arg1	Profile					30:36	the N-Glycosylation Profile	10:36	the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells	10:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	4	46	theme	relative	764:771	arg1	abundance					773:781	the relative abundance	760:781	the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites	760:850	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	5	47	theme	Lewis	1100:1104	arg1	structures					1108:1117	additional Lewis A structures	1089:1117	additional Lewis A structures (BY-2)	1089:1124	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	47	theme	Lewis	1100:1104	arg1	BY-2					1120:1123	BY-2	1120:1123	BY-2	1120:1123	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	2	48	theme	hamster	552:558	arg1	cells					572:576	Chinese hamster ovary (CHO) cells	544:576	Chinese hamster ovary (CHO) cells	544:576	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	4	49	theme	mass	886:889	arg1	spectrometry					891:902	mass spectrometry	886:902	mass spectrometry	886:902	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	4	50	theme	N-glycans	786:794	arg1	abundance					773:781	the relative abundance	760:781	the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites	760:850	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	2	51	dep	consisted	302:310	arg1	comparing					480:488	comparing	480:488	comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells	480:576	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	51	dep	consisted	302:310	arg1	expressing					315:324	expressing	315:324	expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	315:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	52	theme	Yellow-2	440:447	arg1	cells					467:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells	415:471	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	53	theme	Human	383:387	arg1	HMCV					406:409	HMCV	406:409	HMCV	406:409	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	53	theme	Human	383:387	arg1	Cytomegalovirus					389:403	Human Cytomegalovirus	383:403	Human Cytomegalovirus (HMCV)	383:410	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	54	theme	Chinese	544:550	arg1	cells					572:576	Chinese hamster ovary (CHO) cells	544:576	Chinese hamster ovary (CHO) cells	544:576	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	8	55	theme	expression	1494:1503	arg1	system					1505:1510	an alternative expression system	1479:1510	an alternative expression system	1479:1510	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	8	55	theme	expression	1494:1503	arg1	cultures					1467:1474	the BY-2 suspension cultures	1447:1474	the BY-2 suspension cultures	1447:1474	These data thus strengthen the BY-2 suspension cultures as an alternative expression system.					
31370181	4	56	from	abundance	773:781	arg1	15					807:808	15	807:808	15	807:808	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	4	56	from	abundance	773:781	arg1	CHO					825:827	CHO	825:827	CHO	825:827	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	4	56	from	abundance	773:781	arg1	BY-2					811:814	BY-2	811:814	BY-2	811:814	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	4	56	from	abundance	773:781	arg1	17					821:822	17	821:822	17	821:822	We then measured the relative abundance of N-glycans present on 15 (BY-2) and 17 (CHO) out of the 18 N-sites by multienzymatic proteolysis and mass spectrometry.					
31370181	2	57	theme	soluble	336:342	arg1	ectodomain					344:353	the large soluble ectodomain	326:353	the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV)	326:410	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	57	theme	soluble	336:342	arg1	B					371:371	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	3	58	theme	exclusion	717:725	arg1	chromatography					727:740	size exclusion chromatography	712:740	size exclusion chromatography	712:740	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	7	59	theme	similar	1275:1281	arg1	conformation					1286:1297	a similar gB conformation	1273:1297	a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility	1273:1367	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	5	60	theme	glycosylation	909:921	arg1	profile					923:929	The glycosylation profile	905:929	The glycosylation profile	905:929	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	2	61	theme	large	330:334	arg1	ectodomain					344:353	the large soluble ectodomain	326:353	the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV)	326:410	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	61	theme	large	330:334	arg1	B					371:371	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	1	62	theme	major	231:235	arg1	prerequisite					237:248	a major prerequisite	229:248	a major prerequisite before their use as vaccines	229:277	The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines.					
31370181	3	63	theme	culture	607:613	arg1	medium					615:620	the BY-2 culture medium	598:620	the BY-2 culture medium	598:620	gB was secreted in the BY-2 culture medium at a concentration of 20 mg/L and directly purified by ammonium sulfate precipitation and size exclusion chromatography.					
31370181	7	64	theme	gB	1283:1284	arg1	conformation					1286:1297	a similar gB conformation	1273:1297	a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility	1273:1367	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	5	65	theme	oligomannosidic	997:1011	arg1	N-glycans					1018:1026	oligomannosidic type N-glycans	997:1026	oligomannosidic type N-glycans	997:1026	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	66	theme	sialic	1157:1162	arg1	CHO					1170:1172	CHO	1170:1172	CHO	1170:1172	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	5	66	theme	sialic	1157:1162	arg1	acid					1164:1167	sialic acid	1157:1167	sialic acid (CHO)	1157:1173	The glycosylation profile differed at each N-site, some sites being occupied exclusively by oligomannosidic type N-glycans and others by complex N-glycans processed in some cases with additional Lewis A structures (BY-2) or with beta-1,4-galactose and sialic acid (CHO).					
31370181	2	67	from	Cytomegalovirus	389:403	arg1	ectodomain					344:353	the large soluble ectodomain	326:353	the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV)	326:410	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	67	from	Cytomegalovirus	389:403	arg1	B					371:371	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	7	68	gly	glycoproteins	1304:1316	arg1	accessibility					1355:1367	site accessibility	1350:1367	site accessibility	1350:1367	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	7	68	gly	glycoproteins	1304:1316	arg1	glycoproteins					1304:1316	glycoproteins	1304:1316	glycoproteins	1304:1316	These results suggest a similar gB conformation when glycoproteins are expressed in plant cells as site accessibility influences the glycosylation profile at each site.					
31370181	0	69	theme	Human	61:65	arg1	Cytomegalovirus					67:81	Human Cytomegalovirus	61:81	Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells	61:131	Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells.					
31370181	2	70	theme	B	371:371	arg1	ectodomain					344:353	the large soluble ectodomain	326:353	the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV)	326:410	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	70	theme	B	371:371	arg1	B					371:371	glycoprotein B	358:371	glycoprotein B (gB)	358:376	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31370181	2	71	theme	glycosylation	494:506	arg1	profile					508:514	its glycosylation profile	490:514	its glycosylation profile	490:514	The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B (gB) from Human Cytomegalovirus (HMCV) in Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells and of comparing its glycosylation profile with that of gB produced in Chinese hamster ovary (CHO) cells.					
31529350	6	0	theme	N-glycan	1088:1095	arg1	analysis					1097:1104	GlycoWorks RapiFluor N-glycan analysis	1067:1104	GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin	1067:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	3	1	theme	ALG12	454:458	arg1	α1,6-mannosyltransferase					460:483	ALG12 α1,6-mannosyltransferase	454:483	ALG12 α1,6-mannosyltransferase	454:483	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	9	2	theme	molecular	1624:1632	arg1	defect					1634:1639	the molecular defect	1620:1639	the molecular defect unraveling the complex clinical phenotype of CDG patients	1620:1697	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	6	3	gly	N-glycosylation	1218:1232	arg2	sites					1234:1238	N-glycosylation sites	1218:1238	N-glycosylation sites	1218:1238	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	3	4	theme	mannose	506:512	arg1	residue					514:520	the eighth mannose residue	495:520	the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum	495:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	1	5	theme	moieties	237:244	arg1	I					183:183	CDG type I	174:183	CDG type I	174:183	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	5	theme	moieties	237:244	arg1	synthesis					163:171	deficient synthesis	153:171	deficient synthesis (CDG type I)	153:184	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	6	theme	CDG	174:176	arg1	I					183:183	CDG type I	174:183	CDG type I	174:183	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	6	theme	CDG	174:176	arg1	synthesis					163:171	deficient synthesis	153:171	deficient synthesis (CDG type I)	153:184	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	9	7	theme	complex	1656:1662	arg1	phenotype					1673:1681	the complex clinical phenotype	1652:1681	the complex clinical phenotype of CDG patients	1652:1697	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	6	8	theme	sites	1234:1238	arg1	underoccupancy					1200:1213	underoccupancy	1200:1213	underoccupancy of N-glycosylation sites	1200:1238	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	3	9	theme	dolichol-PP-oligosaccharide	529:555	arg1	precursor					557:565	the dolichol-PP-oligosaccharide precursor	525:565	the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum	525:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	2	10	from	glycosylation	323:335	arg1	phenotypes					365:374	the clinical phenotypes	352:374	the clinical phenotypes of patients with CDG	352:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	7	11	theme	structures	1395:1404	arg1	amounts					1351:1357	accumulating amounts	1338:1357	accumulating amounts of definite high-mannose and hybrid structures	1338:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	11	theme	structures	1395:1404	arg1	high-mannose					1371:1382	definite high-mannose and hybrid structures	1362:1404	high-mannose	1371:1382	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	11	theme	structures	1395:1404	arg1	structures					1395:1404	definite high-mannose and hybrid structures	1362:1404	structures	1395:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	4	12	theme	severe	612:617	arg1	disease					631:637	a severe multisystem disease	610:637	a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections	610:717	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	4	12	theme	severe	612:617	arg1	ALG12-CDG					597:605	ALG12-CDG	597:605	ALG12-CDG	597:605	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	9	13	theme	CDG	1686:1688	arg1	patients					1690:1697	CDG patients	1686:1697	CDG patients	1686:1697	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	6	14	theme	N-glycosylation	1218:1232	arg1	sites					1234:1238	N-glycosylation sites	1218:1238	N-glycosylation sites	1218:1238	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	3	15	theme	endoplasmic	574:584	arg1	reticulum					586:594	the endoplasmic reticulum	570:594	the endoplasmic reticulum	570:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	8	16	theme	dual	1442:1445	arg1	CDG					1447:1449	a dual CDG	1440:1449	a dual CDG (CDG-I and II defects)	1440:1472	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	7	17	theme	definite	1362:1369	arg1	high-mannose					1371:1382	definite high-mannose and hybrid structures	1362:1404	high-mannose	1371:1382	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	6	18	theme	total	922:926	arg1	N-glycans					934:942	total serum N-glycans	922:942	total serum N-glycans	922:942	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	5	19	with	patient	771:777	arg1	variants					796:803	novel ALG12 variants	784:803	novel ALG12 variants	784:803	We thoroughly investigated the glycophenotype in a patient with novel ALG12 variants and immunodeficiency.					
31529350	5	19	with	patient	771:777	arg1	immunodeficiency					809:824	immunodeficiency	809:824	immunodeficiency	809:824	We thoroughly investigated the glycophenotype in a patient with novel ALG12 variants and immunodeficiency.					
31529350	6	20	theme	type	1185:1188	arg1	defect					1192:1197	a CDG type I defect	1179:1197	a CDG type I defect	1179:1197	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	4	21	theme	serum	672:676	arg1	immunoglobulins					678:692	low to deficient serum immunoglobulins	655:692	low to deficient serum immunoglobulins	655:692	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	6	22	theme	MALDI	999:1003	arg1	MS					1005:1006	MALDI MS	999:1006	MALDI MS	999:1006	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	23	theme	II	1462:1463	arg1	defects					1465:1471	II defects	1462:1471	II defects	1462:1471	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	2	24	from	impact	280:285	arg1	glycosylation					323:335	protein glycosylation	315:335	protein glycosylation	315:335	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	2	24	from	impact	280:285	arg1	turn					344:347	turn	344:347	turn	344:347	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	1	25	attach	linked	246:251	arg2	moieties					237:244	glycan moieties	230:244	glycan moieties linked to protein and lipids	230:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	25	attach	linked	246:251	arg1	protein					256:262	protein	256:262	protein	256:262	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	25	attach	linked	246:251	arg1	lipids					268:273	lipids	268:273	lipids	268:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	4	26	dep	immunoglobulins	678:692	arg1	to					659:660	to	659:660	to	659:660	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	6	27	dep	such	1059:1062	arg1	showed					1155:1160	showed	1155:1160	showed	1155:1160	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	0	28	theme	ALG12-CDG	0:8	arg1	insights					32:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights endorse the molecular defect.					
31529350	1	29	theme	glycosylation	95:107	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	29	theme	glycosylation	95:107	arg1	diseases					127:134	genetic diseases	119:134	genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids	119:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	2	30	gly	glycosylation	323:335	arg1	phenotypes					365:374	the clinical phenotypes	352:374	the clinical phenotypes of patients with CDG	352:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	2	31	theme	patients	379:386	arg1	phenotypes					365:374	the clinical phenotypes	352:374	the clinical phenotypes of patients with CDG	352:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	0	32	theme	novel	11:15	arg1	insights					32:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights endorse the molecular defect.					
31529350	6	33	theme	spectrometry-based	1029:1046	arg1	such					1059:1062	such	1059:1062	such	1059:1062	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	33	theme	spectrometry-based	1029:1046	arg1	protocols					1048:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	34	theme	mass	1024:1027	arg1	such					1059:1062	such	1059:1062	such	1059:1062	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	34	theme	mass	1024:1027	arg1	protocols					1048:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	5	35	theme	ALG12	790:794	arg1	variants					796:803	novel ALG12 variants	784:803	novel ALG12 variants	784:803	We thoroughly investigated the glycophenotype in a patient with novel ALG12 variants and immunodeficiency.					
31529350	8	36	dep	CDG	1447:1449	arg1	CDG-I					1452:1456	CDG-I	1452:1456	CDG-I	1452:1456	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	8	36	dep	CDG	1447:1449	arg1	defects					1465:1471	II defects	1462:1471	II defects	1462:1471	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	6	37	theme	first	868:872	arg1	test					879:882	first line test	868:882	first line test for CDG	868:890	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	38	theme	innovative	1013:1022	arg1	such					1059:1062	such	1059:1062	such	1059:1062	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	38	theme	innovative	1013:1022	arg1	protocols					1048:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	39	theme	total	1536:1540	arg1	serum					1542:1546	total serum and IgG N-glycans	1536:1564	serum	1542:1546	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	2	40	theme	defects	304:310	arg1	impact					280:285	The impact	276:285	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG	276:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	6	41	theme	MS.	1126:1128	arg1	transferrin					1143:1153	LC-ESI MS. Intact serum transferrin	1119:1153	LC-ESI MS. Intact serum transferrin	1119:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	42	theme	CDG	1181:1183	arg1	defect					1192:1197	a CDG type I defect	1179:1197	a CDG type I defect	1179:1197	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	43	dep	distinct	1500:1507	arg1	abnormal					1510:1517	abnormal	1510:1517	abnormal	1510:1517	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	6	44	theme	native	845:850	arg1	transferrin					852:862	serum native transferrin	839:862	serum native transferrin	839:862	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	9	45	theme	target	1587:1592	arg1	glycoproteins					1594:1606	target glycoproteins	1587:1606	target glycoproteins	1587:1606	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	7	46	theme	serum	1261:1265	arg1	proteins					1267:1274	serum proteins	1261:1274	serum proteins	1261:1274	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	8	47	theme	N-glycans	1556:1564	arg1	glycosylation					1519:1531	distinct, abnormal glycosylation	1500:1531	distinct, abnormal glycosylation of total serum and IgG N-glycans	1500:1564	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	3	48	theme	α1,6-mannosyltransferase	460:483	arg1	deficiency					440:449	deficiency	440:449	deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum	440:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	1	49	theme	type	218:221	arg1	II					223:224	CDG type II	214:224	CDG type II	214:224	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	49	theme	type	218:221	arg1	processing					202:211	abnormal processing	193:211	abnormal processing (CDG type II)	193:225	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	2	50	theme	protein	315:321	arg1	glycosylation					323:335	protein glycosylation	315:335	protein glycosylation	315:335	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	6	51	theme	serum	908:912	arg1	IgG					914:916	serum IgG	908:916	serum IgG	908:916	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	52	theme	RapiFluor	1078:1086	arg1	analysis					1097:1104	GlycoWorks RapiFluor N-glycan analysis	1067:1104	GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin	1067:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	3	53	theme	eighth	499:504	arg1	residue					514:520	the eighth mannose residue	495:520	the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum	495:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	1	54	theme	glycan	230:235	arg1	moieties					237:244	glycan moieties	230:244	glycan moieties linked to protein and lipids	230:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	9	55	gly	glycoproteins	1594:1606	arg1	glycoproteins					1594:1606	target glycoproteins	1587:1606	target glycoproteins	1587:1606	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	2	56	with	patients	379:386	arg1	CDG					393:395	CDG	393:395	CDG	393:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	1	57	theme	type	178:181	arg1	I					183:183	CDG type I	174:183	CDG type I	174:183	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	57	theme	type	178:181	arg1	synthesis					163:171	deficient synthesis	153:171	deficient synthesis (CDG type I)	153:184	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	6	58	theme	consolidated	964:975	arg1	such					1059:1062	such	1059:1062	such	1059:1062	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	58	theme	consolidated	964:975	arg1	protocols					1048:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols	964:1056	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	9	59	theme	clinical	1664:1671	arg1	phenotype					1673:1681	the complex clinical phenotype	1652:1681	the complex clinical phenotype of CDG patients	1652:1697	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	4	60	theme	low	655:657	arg1	immunoglobulins					678:692	low to deficient serum immunoglobulins	655:692	low to deficient serum immunoglobulins	655:692	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	6	61	theme	serum	1137:1141	arg1	transferrin					1143:1153	LC-ESI MS. Intact serum transferrin	1119:1153	LC-ESI MS. Intact serum transferrin	1119:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	1	62	theme	abnormal	193:200	arg1	II					223:224	CDG type II	214:224	CDG type II	214:224	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	62	theme	abnormal	193:200	arg1	processing					202:211	abnormal processing	193:211	abnormal processing (CDG type II)	193:225	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	9	63	theme	patients	1690:1697	arg1	phenotype					1673:1681	the complex clinical phenotype	1652:1681	the complex clinical phenotype of CDG patients	1652:1697	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	7	64	theme	hybrid	1388:1393	arg1	structures					1395:1404	definite high-mannose and hybrid structures	1362:1404	structures	1395:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	4	65	theme	multisystem	619:629	arg1	disease					631:637	a severe multisystem disease	610:637	a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections	610:717	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	4	65	theme	multisystem	619:629	arg1	ALG12-CDG					597:605	ALG12-CDG	597:605	ALG12-CDG	597:605	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	6	66	theme	serum	928:932	arg1	N-glycans					934:942	total serum N-glycans	922:942	total serum N-glycans	922:942	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	7	67	theme	high-mannose	1371:1382	arg1	amounts					1351:1357	accumulating amounts	1338:1357	accumulating amounts of definite high-mannose and hybrid structures	1338:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	67	theme	high-mannose	1371:1382	arg1	high-mannose					1371:1382	definite high-mannose and hybrid structures	1362:1404	high-mannose	1371:1382	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	67	theme	high-mannose	1371:1382	arg1	structures					1395:1404	definite high-mannose and hybrid structures	1362:1404	structures	1395:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	4	68	theme	recurrent	698:706	arg1	infections					708:717	recurrent infections	698:717	recurrent infections	698:717	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	3	69	from	residue	514:520	arg1	precursor					557:565	the dolichol-PP-oligosaccharide precursor	525:565	the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum	525:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	1	70	theme	genetic	119:125	arg1	diseases					127:134	genetic diseases	119:134	genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids	119:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	70	theme	genetic	119:125	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	6	71	dep	consolidated	964:975	arg1	analysis					987:994	N-glycan analysis	978:994	N-glycan analysis by MALDI MS	978:1006	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	6	72	theme	I	1190:1190	arg1	defect					1192:1197	a CDG type I defect	1179:1197	a CDG type I defect	1179:1197	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	7	73	theme	accumulating	1338:1349	arg1	amounts					1351:1357	accumulating amounts	1338:1357	accumulating amounts of definite high-mannose and hybrid structures	1338:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	73	theme	accumulating	1338:1349	arg1	high-mannose					1371:1382	definite high-mannose and hybrid structures	1362:1404	high-mannose	1371:1382	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	7	73	theme	accumulating	1338:1349	arg1	structures					1395:1404	definite high-mannose and hybrid structures	1362:1404	structures	1395:1404	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	4	74	theme	deficient	662:670	arg1	immunoglobulins					678:692	low to deficient serum immunoglobulins	655:692	low to deficient serum immunoglobulins	655:692	ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections.					
31529350	1	75	theme	deficient	153:161	arg1	I					183:183	CDG type I	174:183	CDG type I	174:183	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	75	theme	deficient	153:161	arg1	synthesis					163:171	deficient synthesis	153:171	deficient synthesis (CDG type I)	153:184	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	7	76	dep	total	1255:1259	arg1	proteins					1267:1274	serum proteins	1261:1274	serum proteins	1261:1274	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	6	77	theme	N-glycan	978:985	arg1	analysis					987:994	N-glycan analysis	978:994	N-glycan analysis by MALDI MS	978:1006	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	1	78	theme	Congenital	71:80	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	78	theme	Congenital	71:80	arg1	diseases					127:134	genetic diseases	119:134	genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids	119:273	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	6	79	theme	GlycoWorks	1067:1076	arg1	analysis					1097:1104	GlycoWorks RapiFluor N-glycan analysis	1067:1104	GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin	1067:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	80	theme	distinct	1500:1507	arg1	glycosylation					1519:1531	distinct, abnormal glycosylation	1500:1531	distinct, abnormal glycosylation of total serum and IgG N-glycans	1500:1564	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	7	81	theme	specific	1306:1313	arg1	changes					1315:1321	some specific changes	1301:1321	some specific changes	1301:1321	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	3	82	from	precursor	557:565	arg1	reticulum					586:594	the endoplasmic reticulum	570:594	the endoplasmic reticulum	570:594	ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum.					
31529350	0	83	theme	glycophenotype	17:30	arg1	insights					32:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights	0:39	ALG12-CDG: novel glycophenotype insights endorse the molecular defect.					
31529350	6	84	theme	protocols	1048:1056	arg1	combination					949:959	a combination	947:959	a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites	947:1238	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	85	gly	glycosylation	1519:1531	arg1	serum					1542:1546	total serum and IgG N-glycans	1536:1564	serum	1542:1546	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	8	85	gly	glycosylation	1519:1531	arg1	N-glycans					1556:1564	total serum and IgG N-glycans	1536:1564	N-glycans	1556:1564	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	5	86	theme	novel	784:788	arg1	variants					796:803	novel ALG12 variants	784:803	novel ALG12 variants	784:803	We thoroughly investigated the glycophenotype in a patient with novel ALG12 variants and immunodeficiency.					
31529350	6	87	theme	line	874:877	arg1	test					879:882	first line test	868:882	first line test for CDG	868:890	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	7	88	theme	IgG	1280:1282	arg1	N-glycans					1284:1292	IgG N-glycans	1280:1292	IgG N-glycans	1280:1292	Surprisingly, total serum proteins and IgG N-glycans showed some specific changes, consisting in accumulating amounts of definite high-mannose and hybrid structures.					
31529350	8	89	theme	serum	1542:1546	arg1	glycosylation					1519:1531	distinct, abnormal glycosylation	1500:1531	distinct, abnormal glycosylation of total serum and IgG N-glycans	1500:1564	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	2	90	theme	clinical	356:363	arg1	phenotypes					365:374	the clinical phenotypes	352:374	the clinical phenotypes of patients with CDG	352:395	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	0	91	theme	molecular	53:61	arg1	defect					63:68	the molecular defect	49:68	the molecular defect	49:68	ALG12-CDG: novel glycophenotype insights endorse the molecular defect.					
31529350	9	92	theme	Glycan	1567:1572	arg1	profiling					1574:1582	Glycan profiling	1567:1582	Glycan profiling of target glycoproteins	1567:1606	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
31529350	6	93	theme	Intact	1130:1135	arg1	transferrin					1143:1153	LC-ESI MS. Intact serum transferrin	1119:1153	LC-ESI MS. Intact serum transferrin	1119:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	8	94	theme	IgG	1552:1554	arg1	N-glycans					1556:1564	total serum and IgG N-glycans	1536:1564	N-glycans	1556:1564	As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans.					
31529350	2	95	theme	molecular	294:302	arg1	defects					304:310	the molecular defects	290:310	the molecular defects	290:310	The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood.					
31529350	6	96	theme	LC-ESI	1119:1124	arg1	transferrin					1143:1153	LC-ESI MS. Intact serum transferrin	1119:1153	LC-ESI MS. Intact serum transferrin	1119:1153	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	1	97	theme	CDG	214:216	arg1	II					223:224	CDG type II	214:224	CDG type II	214:224	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	1	97	theme	CDG	214:216	arg1	processing					202:211	abnormal processing	193:211	abnormal processing (CDG type II)	193:225	Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids.					
31529350	6	98	theme	serum	839:843	arg1	transferrin					852:862	serum native transferrin	839:862	serum native transferrin	839:862	We analyzed serum native transferrin, as first line test for CDG and we profiled serum IgG and total serum N-glycans by a combination of consolidated (N-glycan analysis by MALDI MS) and innovative mass spectrometry-based protocols, such as GlycoWorks RapiFluor N-glycan analysis coupled with LC-ESI MS. Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites.					
31529350	9	99	theme	glycoproteins	1594:1606	arg1	profiling					1574:1582	Glycan profiling	1567:1582	Glycan profiling of target glycoproteins	1567:1606	Glycan profiling of target glycoproteins may endorse the molecular defect unraveling the complex clinical phenotype of CDG patients.					
29792883	7	0	theme	fucosylation	1199:1210	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	0	theme	fucosylation	1199:1210	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	4	1	theme	controls	573:580	arg1	N-glycomes					504:513	the total plasma N-glycomes	487:513	the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls	487:580	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	5	2	theme	Plasma	665:670	arg1	samples					672:678	Plasma samples	665:678	Plasma samples	665:678	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	10	3	from	that	1823:1826	arg1	plasma					1789:1794	plasma	1789:1794	plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked)	1789:1933	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	11	4	theme	potent	2034:2039	arg1	medication					2041:2050	a more potent medication	2027:2050	a more potent medication	2027:2050	Glycosylation patterns were associated with disease location and progression, the need for a more potent medication, and surgery.					
29792883	4	5	theme	mass	585:588	arg1	spectrometry					590:601	mass spectrometry	585:601	mass spectrometry with a linkage-specific sialic acid derivatization technique	585:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	10	6	theme	lower	1875:1879	arg1	galactosylation					1881:1895	lower galactosylation	1875:1895	lower galactosylation	1875:1895	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	2	7	theme	common	251:256	arg1	modification					288:299	a common and complex posttranslational modification	249:299	a common and complex posttranslational modification of proteins that affects their structure and activity	249:353	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	2	7	theme	common	251:256	arg1	Glycosylation					232:244	Glycosylation	232:244	Glycosylation	232:244	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	9	8	theme	decreased	1608:1616	arg1	abundance					1627:1635	a decreased relative abundance	1606:1635	a decreased relative abundance of hybrid and high-mannose structures	1606:1673	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	8	theme	decreased	1608:1616	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	11	9	theme	Glycosylation	1936:1948	arg1	patterns					1950:1957	Glycosylation patterns	1936:1957	Glycosylation patterns	1936:1957	Glycosylation patterns were associated with disease location and progression, the need for a more potent medication, and surgery.					
29792883	10	10	theme	higher	1902:1907	arg1	sialylation					1909:1919	higher sialylation	1902:1919	higher sialylation (α2,3-linked)	1902:1933	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	4	11	with	patients	523:530	arg1	diseases					556:563	inflammatory bowel diseases	537:563	inflammatory bowel diseases	537:563	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	5	12	theme	validation	852:861	arg1	cohort					863:868	validation cohort	852:868	validation cohort	852:868	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	9	13	theme	hybrid	1640:1645	arg1	abundance					1627:1635	a decreased relative abundance	1606:1635	a decreased relative abundance of hybrid and high-mannose structures	1606:1673	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	13	theme	hybrid	1640:1645	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	5	14	with	controls	799:806	arg1	disease					777:783	inflammatory bowel disease	758:783	inflammatory bowel disease [IBD]	758:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	4	15	theme	acid	634:637	arg1	technique					654:662	a linkage-specific sialic acid derivatization technique	608:662	a linkage-specific sialic acid derivatization technique	608:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	9	16	contain	had	1537:1539	arg1	samples					1506:1512	Plasma samples	1499:1512	Plasma samples from patients with IBD	1499:1535	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	16	contain	had	1537:1539	arg2	abundance					1550:1558	a higher abundance	1541:1558	a higher abundance of large-size glycans	1541:1580	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	7	17	theme	Common	1042:1047	arg1	features					1049:1056	Common features	1042:1056	Common features shared by the glycan compositions	1042:1090	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	2	18	theme	complex	262:268	arg1	modification					288:299	a common and complex posttranslational modification	249:299	a common and complex posttranslational modification of proteins that affects their structure and activity	249:353	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	2	18	theme	complex	262:268	arg1	Glycosylation					232:244	Glycosylation	232:244	Glycosylation	232:244	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	1	19	theme	colitis	187:193	arg1	detection					138:146	early detection	132:146	early detection of Crohn's disease (CD) and ulcerative colitis (UC)	132:198	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	9	20	theme	high-mannose	1651:1662	arg1	structures					1664:1673	high-mannose structures	1651:1673	high-mannose structures	1651:1673	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	5	21	theme	discovery	721:729	arg1	cohort					731:736	discovery cohort	721:736	discovery cohort	721:736	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	21	theme	discovery	721:729	arg1	patients					744:751	1989 patients	739:751	1989 patients with inflammatory bowel disease [IBD]	739:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	21	theme	discovery	721:729	arg1	controls					799:806	570 controls	795:806	570 controls	795:806	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	4	22	with	spectrometry	590:601	arg1	technique					654:662	a linkage-specific sialic acid derivatization technique	608:662	a linkage-specific sialic acid derivatization technique	608:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	5	23	theme	IBD	884:886	arg1	controls					896:903	426 controls	892:903	426 controls	892:903	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	23	theme	IBD	884:886	arg1	cases					875:879	646 cases	871:879	646 cases of IBD	871:886	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	8	24	theme	protein	1412:1418	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	24	theme	protein	1412:1418	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	24	theme	protein	1412:1418	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	25	theme	plasma	1274:1279	arg1	age					1297:1299	plasma N-glycomes with age	1274:1299	plasma N-glycomes with age	1274:1299	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	5	26	dep	cohort	863:868	arg1	controls					896:903	426 controls	892:903	426 controls	892:903	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	26	dep	cohort	863:868	arg1	cases					875:879	646 cases	871:879	646 cases of IBD	871:886	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	9	27	theme	lower	1696:1700	arg1	galactosylation					1702:1716	lower galactosylation	1696:1716	lower galactosylation	1696:1716	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	27	theme	lower	1696:1700	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	8	28	theme	age	1297:1299	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	28	theme	age	1297:1299	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	28	theme	age	1297:1299	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	5	29	theme	inflammatory	758:769	arg1	disease					777:783	inflammatory bowel disease	758:783	inflammatory bowel disease [IBD]	758:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	10	30	dep	sialylation	1909:1919	arg1	α2,3-linked					1922:1932	α2,3-linked	1922:1932	α2,3-linked	1922:1932	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	8	31	theme	sex	1302:1304	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	31	theme	sex	1302:1304	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	31	theme	sex	1302:1304	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	1	32	theme	disease	159:165	arg1	detection					138:146	early detection	132:146	early detection of Crohn's disease (CD) and ulcerative colitis (UC)	132:198	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	9	33	theme	higher	1723:1728	arg1	sialylation					1730:1740	higher sialylation	1723:1740	higher sialylation	1723:1740	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	33	theme	higher	1723:1728	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	4	34	theme	plasma	497:502	arg1	N-glycomes					504:513	the total plasma N-glycomes	487:513	the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls	487:580	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	8	35	theme	CD	1307:1308	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	35	theme	CD	1307:1308	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	35	theme	CD	1307:1308	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	7	36	theme	complex-type	1164:1175	arg1	glycans					1177:1183	complex-type glycans	1164:1183	complex-type glycans	1164:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	5	37	dep	acquired	685:692	arg1	cohort					863:868	validation cohort	852:868	validation cohort	852:868	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	8	38	theme	linear	1459:1464	arg1	regression					1479:1488	linear and logistic regression	1459:1488	regression	1479:1488	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	39	theme	UC	1311:1312	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	39	theme	UC	1311:1312	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	39	theme	UC	1311:1312	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	7	40	theme	antennae	1152:1159	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	40	theme	antennae	1152:1159	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	1	41	theme	BACKGROUND	88:97	arg1	Biomarkers					106:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	3	42	theme	plasma	368:373	arg1	N-glycosylation profiles					375:398	plasma N-glycosylation profiles	368:398	plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls)	368:464	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	8	43	theme	logistic	1470:1477	arg1	regression					1479:1488	linear and logistic regression	1459:1488	regression	1479:1488	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	0	44	theme	Plasma	0:5	arg1	Signatures					16:25	Plasma N-Glycan Signatures	0:25	Plasma N-Glycan Signatures	0:25	Plasma N-Glycan Signatures Are Associated With Features of Inflammatory Bowel Diseases.					
29792883	1	45	theme	AIMS	101:104	arg1	Biomarkers					106:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	6	46	theme	software	1021:1028	arg1	MassyTools					1030:1039	the software MassyTools	1017:1039	the software MassyTools	1017:1039	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	4	47	theme	inflammatory	537:548	arg1	diseases					556:563	inflammatory bowel diseases	537:563	inflammatory bowel diseases	537:563	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	8	48	theme	parameters	1331:1340	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	48	theme	parameters	1331:1340	arg1	Associations					1258:1269	Associations	1258:1269	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication	1258:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	48	theme	parameters	1331:1340	arg1	level					1392:1396	level	1392:1396	level of C-reactive protein	1392:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	9	49	dep	controls	1596:1603	arg1	α2,6-linked					1753:1763	α2,6-linked	1753:1763	α2,6-linked	1753:1763	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	49	dep	controls	1596:1603	arg1	α2,3-					1743:1747	α2,3-	1743:1747	α2,3-	1743:1747	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	4	50	dep	METHODS	467:473	arg1	analyzed					478:485	analyzed	478:485	analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique	478:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	12	51	theme	independent	2107:2117	arg1	cohort					2119:2124	a large independent cohort	2099:2124	a large independent cohort	2099:2124	These results were replicated in a large independent cohort.					
29792883	6	52	theme	raw	1003:1005	arg1	data					1007:1010	the raw data	999:1010	the raw data with the software MassyTools	999:1039	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	0	53	theme	Bowel	72:76	arg1	Diseases					78:85	Bowel Diseases	72:85	Bowel Diseases	72:85	Plasma N-Glycan Signatures Are Associated With Features of Inflammatory Bowel Diseases.					
29792883	6	54	with	data	1007:1010	arg1	MassyTools					1030:1039	the software MassyTools	1017:1039	the software MassyTools	1017:1039	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	7	55	theme	sialylation	1245:1255	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	55	theme	sialylation	1245:1255	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	4	56	theme	patients	523:530	arg1	N-glycomes					504:513	the total plasma N-glycomes	487:513	the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls	487:580	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	9	57	theme	large-size	1563:1572	arg1	glycans					1574:1580	large-size glycans	1563:1580	large-size glycans	1563:1580	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	7	58	theme	glycans	1177:1183	arg1	antennae					1152:1159	antennae	1152:1159	antennae of complex-type glycans	1152:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	1	59	theme	patient	214:220	arg1	outcomes					222:229	patient outcomes	214:229	patient outcomes	214:229	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	7	60	theme	glycan	1072:1077	arg1	compositions					1079:1090	the glycan compositions	1068:1090	the glycan compositions	1068:1090	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	14	61	used	used	2357:2360	arg2	profiles					2339:2346	These profiles	2333:2346	These profiles	2333:2346	These profiles might be used in diagnosis and for predicting patients' responses to treatment.					
29792883	3	62	with	patients	408:415	arg1	UC					428:429	UC	428:429	UC	428:429	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	3	62	with	patients	408:415	arg1	CD					422:423	CD	422:423	CD	422:423	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	5	63	theme	United	837:842	arg1	States					844:849	the United States	833:849	the United States	833:849	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	9	64	theme	relative	1618:1625	arg1	abundance					1627:1635	a decreased relative abundance	1606:1635	a decreased relative abundance of hybrid and high-mannose structures	1606:1673	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	64	theme	relative	1618:1625	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	10	65	theme	higher	1857:1862	arg1	bisection					1864:1872	higher bisection	1857:1872	higher bisection	1857:1872	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	13	66	theme	disease	2288:2294	arg1	features					2296:2303	disease features	2288:2303	disease features	2288:2303	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	3	67	with	individuals	443:453	arg1	UC					428:429	UC	428:429	UC	428:429	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	3	67	with	individuals	443:453	arg1	CD					422:423	CD	422:423	CD	422:423	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	2	68	theme	posttranslational	270:286	arg1	modification					288:299	a common and complex posttranslational modification	249:299	a common and complex posttranslational modification of proteins that affects their structure and activity	249:353	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	2	68	theme	posttranslational	270:286	arg1	Glycosylation					232:244	Glycosylation	232:244	Glycosylation	232:244	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	5	69	theme	medical	815:821	arg1	center					823:828	1 medical center	813:828	1 medical center	813:828	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	6	70	dep	glycoforms	919:928	arg1	met					930:932	met	930:932	met	930:932	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	5	71	dep	disease	777:783	arg1	[IBD					785:788	[IBD	785:788	[IBD	785:788	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	13	72	theme	individuals	2215:2225	arg1	N-glycomes					2201:2210	total plasma N-glycomes	2188:2210	total plasma N-glycomes of individuals with vs without IBD	2188:2245	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	4	73	theme	sialic	627:632	arg1	technique					654:662	a linkage-specific sialic acid derivatization technique	608:662	a linkage-specific sialic acid derivatization technique	608:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	6	74	theme	Sixty-three	907:917	arg1	glycoforms					919:928	Sixty-three glycoforms	907:928	Sixty-three glycoforms met our criteria for relative quantification	907:973	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	9	75	theme	structures	1664:1673	arg1	abundance					1627:1635	a decreased relative abundance	1606:1635	a decreased relative abundance of hybrid and high-mannose structures	1606:1673	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	75	theme	structures	1664:1673	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	8	76	theme	C-reactive	1401:1410	arg1	protein					1412:1418	C-reactive protein	1401:1418	C-reactive protein	1401:1418	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	5	77	with	patients	744:751	arg1	disease					777:783	inflammatory bowel disease	758:783	inflammatory bowel disease [IBD]	758:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	4	78	theme	derivatization	639:652	arg1	technique					654:662	a linkage-specific sialic acid derivatization technique	608:662	a linkage-specific sialic acid derivatization technique	608:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	7	79	link	derived	1112:1118	arg1	traits					1120:1125	78 derived traits	1109:1125	78 derived traits	1109:1125	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	79	link	derived	1112:1118	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	79	link	derived	1112:1118	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	9	80	theme	lower	1676:1680	arg1	fucosylation					1682:1693	lower fucosylation	1676:1693	lower fucosylation	1676:1693	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	9	80	theme	lower	1676:1680	arg1	controls					1596:1603	controls	1596:1603	controls	1596:1603	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	1	81	theme	early	132:136	arg1	detection					138:146	early detection	132:146	early detection of Crohn's disease (CD) and ulcerative colitis (UC)	132:198	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	8	82	theme	sedimentation	1425:1437	arg1	rate					1439:1442	sedimentation rate	1425:1442	sedimentation rate	1425:1442	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	9	83	dep	RESULTS	1491:1497	arg1	had					1537:1539	had	1537:1539	had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked)	1537:1764	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	4	84	with	controls	573:580	arg1	diseases					556:563	inflammatory bowel diseases	537:563	inflammatory bowel diseases	537:563	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	1	85	theme	ulcerative	176:185	arg1	colitis					187:193	ulcerative colitis	176:193	ulcerative colitis (UC)	176:198	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	1	85	theme	ulcerative	176:185	arg1	UC					196:197	UC	196:197	UC	196:197	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	13	86	theme	plasma	2194:2199	arg1	N-glycomes					2201:2210	total plasma N-glycomes	2188:2210	total plasma N-glycomes of individuals with vs without IBD	2188:2245	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	9	87	from	patients	1519:1526	arg1	samples					1506:1512	Plasma samples	1499:1512	Plasma samples from patients with IBD	1499:1535	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	4	88	theme	total	491:495	arg1	N-glycomes					504:513	the total plasma N-glycomes	487:513	the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls	487:580	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	9	89	with	patients	1519:1526	arg1	IBD					1533:1535	IBD	1533:1535	IBD	1533:1535	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	8	90	theme	N-glycomes with	1281:1295	arg1	age					1297:1299	plasma N-glycomes with age	1274:1299	plasma N-glycomes with age	1274:1299	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	10	91	with	patients	1831:1838	arg1	UC					1845:1846	UC	1845:1846	UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked)	1845:1933	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	5	92	theme	bowel	771:775	arg1	disease					777:783	inflammatory bowel disease	758:783	inflammatory bowel disease [IBD]	758:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	11	93	dep	disease	1980:1986	arg1	location					1988:1995	location	1988:1995	location	1988:1995	Glycosylation patterns were associated with disease location and progression, the need for a more potent medication, and surgery.					
29792883	5	94	dep	Italy	714:718	arg1	cohort					731:736	discovery cohort	721:736	discovery cohort	721:736	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	94	dep	Italy	714:718	arg1	patients					744:751	1989 patients	739:751	1989 patients with inflammatory bowel disease [IBD]	739:789	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	94	dep	Italy	714:718	arg1	controls					799:806	570 controls	795:806	570 controls	795:806	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	6	95	theme	relative	951:958	arg1	quantification					960:973	relative quantification	951:973	relative quantification	951:973	Sixty-three glycoforms met our criteria for relative quantification and were extracted from the raw data with the software MassyTools.					
29792883	13	96	dep	performed	2142:2150	arg1	identify					2254:2261	identify	2254:2261	to identify patterns associated with disease features and the need for treatment	2251:2330	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	13	96	dep	performed	2142:2150	arg1	compare					2180:2186	compare	2180:2186	to compare total plasma N-glycomes of individuals with vs without IBD	2177:2245	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	9	97	theme	Plasma	1499:1504	arg1	samples					1506:1512	Plasma samples	1499:1512	Plasma samples from patients with IBD	1499:1535	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	13	98	dep	CONCLUSIONS	2127:2137	arg1	performed					2142:2150	performed	2142:2150	performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment	2142:2330	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	3	99	theme	healthy	435:441	arg1	controls					456:463	controls	456:463	controls	456:463	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	3	99	theme	healthy	435:441	arg1	individuals					443:453	healthy individuals	435:453	healthy individuals (controls)	435:464	We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls).					
29792883	0	100	theme	N-Glycan	7:14	arg1	Signatures					16:25	Plasma N-Glycan Signatures	0:25	Plasma N-Glycan Signatures	0:25	Plasma N-Glycan Signatures Are Associated With Features of Inflammatory Bowel Diseases.					
29792883	1	101	theme	&	99:99	arg1	Biomarkers					106:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers	88:115	BACKGROUND & AIMS Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes.					
29792883	4	102	theme	bowel	550:554	arg1	diseases					556:563	inflammatory bowel diseases	537:563	inflammatory bowel diseases	537:563	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
29792883	8	103	theme	IBD-related	1319:1329	arg1	medication					1380:1389	medication	1380:1389	medication	1380:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	103	theme	IBD-related	1319:1329	arg1	surgery					1368:1374	surgery	1368:1374	surgery	1368:1374	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	103	theme	IBD-related	1319:1329	arg1	location					1358:1365	disease location	1350:1365	disease location	1350:1365	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	8	103	theme	IBD-related	1319:1329	arg1	parameters					1331:1340	IBD-related parameters	1319:1340	IBD-related parameters such as disease location, surgery and medication	1319:1389	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	7	104	theme	derived	1112:1118	arg1	traits					1120:1125	78 derived traits	1109:1125	78 derived traits	1109:1125	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	104	theme	derived	1112:1118	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	104	theme	derived	1112:1118	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	13	105	theme	total	2188:2192	arg1	N-glycomes					2201:2210	total plasma N-glycomes	2188:2210	total plasma N-glycomes of individuals with vs without IBD	2188:2245	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	12	106	theme	large	2101:2105	arg1	cohort					2119:2124	a large independent cohort	2099:2124	a large independent cohort	2099:2124	These results were replicated in a large independent cohort.					
29792883	9	107	theme	higher	1543:1548	arg1	abundance					1550:1558	a higher abundance	1541:1558	a higher abundance of large-size glycans	1541:1580	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	10	108	with	patients	1801:1808	arg1	CD					1815:1816	CD	1815:1816	CD	1815:1816	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	8	109	theme	disease	1350:1356	arg1	location					1358:1365	disease location	1350:1365	disease location	1350:1365	Associations of plasma N-glycomes with age, sex, CD, UC, and IBD-related parameters such as disease location, surgery and medication, level of C-reactive protein, and sedimentation rate were tested by linear and logistic regression.					
29792883	13	110	theme	high-throughput	2152:2166	arg1	analysis					2168:2175	high-throughput analysis	2152:2175	high-throughput analysis	2152:2175	CONCLUSIONS We performed high-throughput analysis to compare total plasma N-glycomes of individuals with vs without IBD and to identify patterns associated with disease features and the need for treatment.					
29792883	7	111	theme	galactosylation	1224:1238	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	111	theme	galactosylation	1224:1238	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	2	112	theme	proteins	304:311	arg1	modification					288:299	a common and complex posttranslational modification	249:299	a common and complex posttranslational modification of proteins that affects their structure and activity	249:353	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	2	112	theme	proteins	304:311	arg1	Glycosylation					232:244	Glycosylation	232:244	Glycosylation	232:244	Glycosylation is a common and complex posttranslational modification of proteins that affects their structure and activity.					
29792883	5	113	from	hospitals	701:709	arg1	Italy					714:718	Italy	714:718	Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls)	714:807	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	5	113	from	hospitals	701:709	arg1	center					823:828	1 medical center	813:828	1 medical center	813:828	Plasma samples were acquired from 2 hospitals in Italy (discovery cohort, 1989 patients with inflammatory bowel disease [IBD] and 570 controls) and 1 medical center in the United States (validation cohort, 646 cases of IBD and 426 controls).					
29792883	10	114	from	patients	1801:1808	arg1	plasma					1789:1794	plasma	1789:1794	plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked)	1789:1933	We could discriminate plasma from patients with CD from that of patients with UC based on higher bisection, lower galactosylation, and higher sialylation (α2,3-linked).					
29792883	9	115	theme	glycans	1574:1580	arg1	abundance					1550:1558	a higher abundance	1541:1558	a higher abundance of large-size glycans	1541:1580	RESULTS Plasma samples from patients with IBD had a higher abundance of large-size glycans compared with controls, a decreased relative abundance of hybrid and high-mannose structures, lower fucosylation, lower galactosylation, and higher sialylation (α2,3- and α2,6-linked).					
29792883	7	116	theme	bisection	1213:1221	arg1	levels					1189:1194	levels	1189:1194	levels of fucosylation, bisection, galactosylation, and sialylation	1189:1255	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	7	116	theme	bisection	1213:1221	arg1	number					1142:1147	the number	1138:1147	the number of antennae of complex-type glycans	1138:1183	Common features shared by the glycan compositions were combined in 78 derived traits, including the number of antennae of complex-type glycans and levels of fucosylation, bisection, galactosylation, and sialylation.					
29792883	4	117	theme	linkage-specific	610:625	arg1	technique					654:662	a linkage-specific sialic acid derivatization technique	608:662	a linkage-specific sialic acid derivatization technique	608:662	METHODS We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique.					
30537828	4	0	dep	peaks	717:721	arg1	found					787:791	found	787:791	are found to be abundant in certain species	783:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	4	0	dep	peaks	717:721	arg1	expressed					738:746	expressed	738:746	were expressed significantly	733:760	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	5	1	theme	Scaled	876:881	arg1	Japanese					894:901	Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	871:932	Japanese	894:901	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	5	2	from	mannose	1009:1015	arg1	Quail					1029:1033	Japanese Quail	1020:1033	Japanese Quail	1020:1033	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	5	3	theme	tetra-antennary	1039:1053	arg1	structure					1062:1070	tetra-antennary glycan structure	1039:1070	tetra-antennary glycan structure	1039:1070	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	3	4	from	species	487:493	arg1	EW					457:458	EW	457:458	EW	457:458	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	4	from	species	487:493	arg1	approach					397:404	an approach to better understand glycan diversity	394:442	an approach to better understand glycan diversity	394:442	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	4	from	species	487:493	arg1	whites					449:454	egg whites	445:454	egg whites (EW) from four different quail species	445:493	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	5	theme	glycoblotting	531:543	arg1	procedure					545:553	the well-established glycoblotting procedure	510:553	the well-established glycoblotting procedure	510:553	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	5	6	theme	egg	923:925	arg1	whites					927:932	Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	871:932	whites	927:932	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	5	7	theme	Mountain	908:915	arg1	whites					927:932	Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	871:932	whites	927:932	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	0	8	from	Expression	52:61	arg1	Species					72:78	Quail Species	66:78	Quail Species	66:78	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	3	9	theme	procedure	545:553	arg1	method					589:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	10	theme	different	471:479	arg1	species					487:493	four different quail species	466:493	four different quail species	466:493	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	1	11	gly	glycoproteins	183:195	arg1	glycoproteins					183:195	glycoproteins	183:195	glycoproteins	183:195	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	2	12	from	intraspecies	326:337	arg1	glycosylation					370:382	glycosylation	370:382	glycosylation	370:382	It is believed that intraspecies and interspecies variations in glycosylation exist.					
30537828	3	13	theme	glycan	427:432	arg1	diversity					434:442	glycan diversity	427:442	glycan diversity	427:442	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	4	14	theme	quail	661:665	arg1	samples					677:683	quail egg white samples	661:683	quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species	661:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	4	15	from	species	819:825	arg1	abundant					799:806	abundant	799:806	abundant	799:806	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	4	16	theme	certain	811:817	arg1	species					819:825	certain species	811:825	certain species	811:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	3	17	theme	well-established	514:529	arg1	procedure					545:553	the well-established glycoblotting procedure	510:553	the well-established glycoblotting procedure	510:553	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	18	theme	egg	445:447	arg1	EW					457:458	EW	457:458	EW	457:458	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	18	theme	egg	445:447	arg1	approach					397:404	an approach to better understand glycan diversity	394:442	an approach to better understand glycan diversity	394:442	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	18	theme	egg	445:447	arg1	whites					449:454	egg whites	445:454	egg whites (EW) from four different quail species	445:493	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	5	19	theme	quail	1082:1086	arg1	species					1088:1094	other quail species	1076:1094	other quail species	1076:1094	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	2	20	from	interspecies	343:354	arg1	glycosylation					370:382	glycosylation	370:382	glycosylation	370:382	It is believed that intraspecies and interspecies variations in glycosylation exist.					
30537828	3	21	theme	glycan	558:563	arg1	enrichment					565:574	a glycan enrichment	556:574	a glycan enrichment	556:574	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	1	22	theme	modulatory	159:168	arg1	functions					170:178	modulatory functions	159:178	modulatory functions	159:178	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	1	23	theme	specific	255:262	arg1	ligands					264:270	specific ligands	255:270	specific ligands for cell-to-microbe interactions	255:303	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	1	24	theme	glycan	85:90	arg1	part					92:95	The glycan part	81:95	The glycan part of glycoproteins	81:112	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	4	25	theme	egg	667:669	arg1	samples					677:683	quail egg white samples	661:683	quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species	661:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	0	26	theme	White	21:25	arg1	Egg					17:19	Egg White	17:25	Egg White	17:25	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	5	27	theme	Quail	917:921	arg1	whites					927:932	Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	871:932	whites	927:932	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	3	28	theme	enrichment	565:574	arg1	method					589:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	1	29	gly	glycoproteins	100:112	arg1	glycoproteins					100:112	glycoproteins	100:112	glycoproteins	100:112	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	2	30	dep	intraspecies	326:337	arg1	variations					356:365	variations	356:365	variations	356:365	It is believed that intraspecies and interspecies variations in glycosylation exist.					
30537828	5	31	from	expressions	977:987	arg1	Quail					1029:1033	Japanese Quail	1020:1033	Japanese Quail	1020:1033	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	1	32	theme	glycoproteins	183:195	arg1	structure					145:153	structure	145:153	structure	145:153	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	1	32	theme	glycoproteins	183:195	arg1	functions					170:178	modulatory functions	159:178	modulatory functions	159:178	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	0	33	theme	Diverse	35:41	arg1	Expression					52:61	Diverse N-Glycan Expression	35:61	Diverse N-Glycan Expression in Quail Species	35:78	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	1	34	theme	cell-to-microbe	276:290	arg1	interactions					292:303	cell-to-microbe interactions	276:303	cell-to-microbe interactions	276:303	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	5	35	theme	glycan	851:856	arg1	profiles					858:865	glycan profiles	851:865	glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	851:932	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	4	36	theme	relevant	701:708	arg1	peaks					717:721	21 relevant glycan peaks	698:721	21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species	698:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	5	37	from	structure	1062:1070	arg1	Quail					1029:1033	Japanese Quail	1020:1033	Japanese Quail	1020:1033	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	4	38	theme	white	671:675	arg1	samples					677:683	quail egg white samples	661:683	quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species	661:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	5	39	dep	Japanese	894:901	arg1	Bobwhite					884:891	Bobwhite	884:891	Bobwhite	884:891	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	5	40	theme	glycan	1055:1060	arg1	structure					1062:1070	tetra-antennary glycan structure	1039:1070	tetra-antennary glycan structure	1039:1070	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	1	41	dep	structure	145:153	arg1	the					141:143	the	141:143	the	141:143	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	4	42	from	abundant	799:806	arg1	species					819:825	certain species	811:825	certain species	811:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	1	43	theme	glycoproteins	100:112	arg1	part					92:95	The glycan part	81:95	The glycan part of glycoproteins	81:112	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30537828	0	44	theme	N-Glycan	43:50	arg1	Expression					52:61	Diverse N-Glycan Expression	35:61	Diverse N-Glycan Expression in Quail Species	35:78	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	5	45	theme	high	1004:1007	arg1	mannose					1009:1015	high mannose	1004:1015	high mannose in Japanese Quail	1004:1033	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	3	46	theme	analysis	580:587	arg1	method					589:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	the well-established glycoblotting procedure, a glycan enrichment and analysis method	510:594	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	3	47	theme	quail	481:485	arg1	species					487:493	four different quail species	466:493	four different quail species	466:493	As an approach to better understand glycan diversity, egg whites (EW) from four different quail species are studied by the well-established glycoblotting procedure, a glycan enrichment and analysis method.					
30537828	5	48	theme	glycan	970:975	arg1	expressions					977:987	glycan expressions	970:987	glycan expressions	970:987	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	4	49	theme	glycan	710:715	arg1	peaks					717:721	21 relevant glycan peaks	698:721	21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species	698:825	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	5	50	theme	unique	946:951	arg1	difference					953:962	a unique difference	944:962	a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species	944:1094	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	4	51	theme	glycan	770:775	arg1	peaks					777:781	10 glycan peaks	767:781	10 glycan peaks	767:781	N-Glycans were classified and the profiles were established for quail egg white samples which showed 21 relevant glycan peaks; 18 peaks were expressed significantly, and 10 glycan peaks are found to be abundant in certain species.					
30537828	0	52	theme	Quail	66:70	arg1	Species					72:78	Quail Species	66:78	Quail Species	66:78	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	5	53	theme	Japanese	1020:1027	arg1	Quail					1029:1033	Japanese Quail	1020:1033	Japanese Quail	1020:1033	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	0	54	theme	Egg	17:19	arg1	Glycoblotting					0:12	Glycoblotting	0:12	Glycoblotting of Egg White	0:25	Glycoblotting of Egg White Reveals Diverse N-Glycan Expression in Quail Species.					
30537828	5	55	theme	other	1076:1080	arg1	species					1088:1094	other quail species	1076:1094	other quail species	1076:1094	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	5	56	theme	Blue	871:874	arg1	Japanese					894:901	Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites	871:932	Japanese	894:901	The result establishes glycan profiles for Blue Scaled, Bobwhite, Japanese, and Mountain Quail egg whites and shows a unique difference among glycan expressions, particularly, high mannose in Japanese Quail and tetra-antennary glycan structure for other quail species.					
30537828	1	57	theme	cell-to-cell	221:232	arg1	interactions					234:245	cell-to-cell interactions	221:245	cell-to-cell interactions	221:245	The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell-to-cell interactions, and as specific ligands for cell-to-microbe interactions.					
30937380	0	0	theme	IgG	85:87	arg1	Antibodies					100:109	Therapeutic IgG Monoclonal Antibodies	73:109	Therapeutic IgG Monoclonal Antibodies	73:109	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	5	1	theme	crystal	804:810	arg1	structures					812:821	crystal structures	804:821	crystal structures of EndoS2 alone and bound to complex and high-mannose glycans	804:883	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	2	2	theme	effector	390:397	arg1	functions					399:407	its effector functions	386:407	its effector functions	386:407	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	2	3	from	glycan	346:351	arg1	Fc					370:371	IgG Fc	366:371	IgG Fc	366:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	0	4	theme	Therapeutic	73:83	arg1	Antibodies					100:109	Therapeutic IgG Monoclonal Antibodies	73:109	Therapeutic IgG Monoclonal Antibodies	73:109	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	5	5	theme	critical	932:939	arg1	loops					941:945	critical loops	932:945	critical loops that shape the binding site of EndoS2	932:983	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	0	6	theme	Antibodies	100:109	arg1	Processing					59:68	Processing	59:68	Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2	59:131	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	0	6	theme	Antibodies	100:109	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Broad Spectrum N-Glycan Specificity	0:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	2	7	theme	N-linked	337:344	arg1	glycan					346:351	the conserved N-linked glycan	323:351	the conserved N-linked glycan at Asn297 on IgG Fc	323:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	2	8	from	Asn297	356:361	arg1	glycan					346:351	the conserved N-linked glycan	323:351	the conserved N-linked glycan at Asn297 on IgG Fc	323:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	4	9	theme	broad	646:650	arg1	complex					696:702	complex	696:702	complex	696:702	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	9	theme	broad	646:650	arg1	hybrid					709:714	hybrid	709:714	hybrid	709:714	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	9	theme	broad	646:650	arg1	high-mannose					682:693	high-mannose	682:693	high-mannose	682:693	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	9	theme	broad	646:650	arg1	range					652:656	a broad range	644:656	a broad range	644:656	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	0	10	theme	Monoclonal	89:98	arg1	Antibodies					100:109	Therapeutic IgG Monoclonal Antibodies	73:109	Therapeutic IgG Monoclonal Antibodies	73:109	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	2	11	theme	conserved	327:335	arg1	glycan					346:351	the conserved N-linked glycan	323:351	the conserved N-linked glycan at Asn297 on IgG Fc	323:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	2	12	theme	IgG	366:368	arg1	Fc					370:371	IgG Fc	366:371	IgG Fc	366:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	2	13	gly	deglycosylates	308:321	arg1	glycan					346:351	the conserved N-linked glycan	323:351	the conserved N-linked glycan at Asn297 on IgG Fc	323:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	3	14	with	antibodies	520:529	arg1	glycosylation					549:561	customizable glycosylation	536:561	customizable glycosylation	536:561	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	6	15	theme	module	1145:1150	arg1	importance					1106:1115	the importance	1102:1115	the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2	1102:1196	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	3	16	theme	point	458:462	arg1	mutants					464:470	specific point mutants	449:470	specific point mutants	449:470	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	5	17	theme	EndoS2	826:831	arg1	structures					812:821	crystal structures	804:821	crystal structures of EndoS2 alone and bound to complex and high-mannose glycans	804:883	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	4	18	from	useful	596:601	arg1	schemes					612:618	these schemes	606:618	these schemes	606:618	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	19	from	schemes	612:618	arg1	useful					596:601	useful	596:601	useful	596:601	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	0	20	theme	Endoglycosidase	114:128	arg1	S2					130:131	Endoglycosidase S2	114:131	Endoglycosidase S2	114:131	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	3	21	gly	glycosylation	549:561	arg1	antibodies					520:529	antibodies	520:529	antibodies with customizable glycosylation for gain of functions	520:583	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	1	22	theme	antibody	192:199	arg1	functions					210:218	antibody effector functions	192:218	antibody effector functions	192:218	Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.					
30937380	0	23	theme	Molecular	0:8	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Broad Spectrum N-Glycan Specificity	0:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	4	24	theme	substrate	749:757	arg1	recognition					759:769	substrate recognition	749:769	substrate recognition	749:769	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	5	25	theme	broad	890:894	arg1	specificity					905:915	the broad N-glycan specificity	886:915	the broad N-glycan specificity	886:915	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	3	26	used	used	482:485	arg2	EndoS2					438:443	EndoS2	438:443	EndoS2	438:443	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	3	26	used	used	482:485	arg2	mutants					464:470	specific point mutants	449:470	specific point mutants	449:470	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	1	27	theme	effector	201:208	arg1	functions					210:218	antibody effector functions	192:218	antibody effector functions	192:218	Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.					
30937380	0	28	theme	Broad	19:23	arg1	Specificity					43:53	Broad Spectrum N-Glycan Specificity	19:53	Broad Spectrum N-Glycan Specificity	19:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	6	29	theme	carbohydrate-binding	1124:1143	arg1	module					1145:1150	the carbohydrate-binding module	1120:1150	the carbohydrate-binding module	1120:1150	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	5	30	theme	binding	962:968	arg1	EndoS2					978:983	EndoS2	978:983	EndoS2	978:983	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	5	30	theme	binding	962:968	arg1	site					970:973	the binding site	958:973	the binding site of EndoS2	958:983	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	5	31	theme	EndoS2	978:983	arg1	EndoS2					978:983	EndoS2	978:983	EndoS2	978:983	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	5	31	theme	EndoS2	978:983	arg1	site					970:973	the binding site	958:973	the binding site of EndoS2	958:983	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	5	32	theme	high-mannose	864:875	arg1	glycans					877:883	high-mannose glycans	864:883	high-mannose glycans	864:883	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	6	33	theme	IgG	1172:1174	arg1	recognition					1176:1186	IgG recognition	1172:1186	IgG recognition by EndoS2	1172:1196	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	2	34	link	N-linked	337:344	arg1	glycan					346:351	the conserved N-linked glycan	323:351	the conserved N-linked glycan at Asn297 on IgG Fc	323:371	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	5	35	theme	N-glycan	896:903	arg1	specificity					905:915	the broad N-glycan specificity	886:915	the broad N-glycan specificity	886:915	We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2.					
30937380	0	36	theme	N-Glycan	34:41	arg1	Specificity					43:53	Broad Spectrum N-Glycan Specificity	19:53	Broad Spectrum N-Glycan Specificity	19:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	6	37	from	importance	1106:1115	arg1	mechanism					1159:1167	the mechanism	1155:1167	the mechanism of IgG recognition by EndoS2	1155:1196	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	0	38	theme	Spectrum	25:32	arg1	Specificity					43:53	Broad Spectrum N-Glycan Specificity	19:53	Broad Spectrum N-Glycan Specificity	19:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	3	39	theme	specific	449:456	arg1	mutants					464:470	specific point mutants	449:470	specific point mutants	449:470	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	2	40	theme	unique	255:260	arg1	EndoS2					294:299	EndoS2	294:299	EndoS2	294:299	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	2	40	theme	unique	255:260	arg1	endo-β-N-acetylglucosaminidase					262:291	a unique endo-β-N-acetylglucosaminidase	253:291	a unique endo-β-N-acetylglucosaminidase	253:291	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	6	41	theme	hydrolytic	999:1008	arg1	experiments					1010:1020	hydrolytic experiments	999:1020	hydrolytic experiments	999:1020	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	4	42	dep	high-mannose	682:693	arg1	types					716:720	types	716:720	types	716:720	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	6	43	theme	recognition	1176:1186	arg1	mechanism					1159:1167	the mechanism	1155:1167	the mechanism of IgG recognition by EndoS2	1155:1196	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	3	44	theme	customizable	536:547	arg1	glycosylation					549:561	customizable glycosylation	536:561	customizable glycosylation	536:561	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	0	45	theme	Specificity	43:53	arg1	Processing					59:68	Processing	59:68	Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2	59:131	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	0	45	theme	Specificity	43:53	arg1	Basis					10:14	Molecular Basis	0:14	Molecular Basis of Broad Spectrum N-Glycan Specificity	0:53	Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.					
30937380	2	46	theme	Streptococcus	221:233	arg1	pyogenes					235:242	Streptococcus pyogenes	221:242	Streptococcus pyogenes	221:242	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	6	47	theme	exchange	1068:1075	arg1	spectrometry					1082:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	1	48	theme	Immunoglobulin	134:147	arg1	IgG					152:154	IgG	152:154	IgG	152:154	Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.					
30937380	1	48	theme	Immunoglobulin	134:147	arg1	G					149:149	Immunoglobulin G	134:149	Immunoglobulin G (IgG) glycosylation	134:169	Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.					
30937380	6	49	theme	mass	1077:1080	arg1	spectrometry					1082:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	1	50	theme	G	149:149	arg1	glycosylation					157:169	Immunoglobulin G (IgG) glycosylation	134:169	Immunoglobulin G (IgG) glycosylation	134:169	Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.					
30937380	4	51	theme	recognition	759:769	arg1	mechanism					736:744	its mechanism	732:744	its mechanism of substrate recognition	732:769	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	6	52	theme	glycosylation	1268:1280	arg1	reactions					1282:1290	customizable glycosylation reactions	1255:1290	customizable glycosylation reactions	1255:1290	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	2	53	theme	immune	423:428	arg1	system					430:435	the immune system	419:435	the immune system	419:435	Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system.					
30937380	6	54	theme	hydrogen-deuterium	1049:1066	arg1	spectrometry					1082:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	hydrogen-deuterium exchange mass spectrometry	1049:1093	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	6	55	theme	domain-swap	1023:1033	arg1	chimeras					1035:1042	domain-swap chimeras	1023:1042	domain-swap chimeras	1023:1042	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	6	56	theme	customizable	1255:1266	arg1	reactions					1282:1290	customizable glycosylation reactions	1255:1290	customizable glycosylation reactions	1255:1290	Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen-deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.					
30937380	3	57	theme	functions	575:583	arg1	gain					567:570	gain	567:570	gain of functions	567:583	EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions.					
30937380	4	58	theme	N-glycans	661:669	arg1	complex					696:702	complex	696:702	complex	696:702	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	58	theme	N-glycans	661:669	arg1	hybrid					709:714	hybrid	709:714	hybrid	709:714	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	58	theme	N-glycans	661:669	arg1	high-mannose					682:693	high-mannose	682:693	high-mannose	682:693	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
30937380	4	58	theme	N-glycans	661:669	arg1	range					652:656	a broad range	644:656	a broad range	644:656	EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood.					
31098625	12	0	theme	site	2064:2067	arg1	specificity					2069:2079	site specificity	2064:2079	site specificity	2064:2079	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	6	1	theme	individual	1103:1112	arg1	proteins					1114:1121	individual proteins	1103:1121	individual proteins	1103:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	3	2	theme	milk	476:479	arg1	proteins					481:488	milk proteins	476:488	milk proteins	476:488	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	10	3	theme	immunoglobulin	1732:1745	arg1	A					1747:1747	secretory immunoglobulin A	1722:1747	secretory immunoglobulin A	1722:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	13	4	theme	human	2195:2199	arg1	milk					2201:2204	human milk	2195:2204	human milk	2195:2204	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	3	5	theme	respective	500:509	arg1	glycosylation					525:537	their respective site-specific glycosylation	494:537	their respective site-specific glycosylation	494:537	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	5	6	theme	breast-milk	877:887	arg1	samples					889:895	231 breast-milk samples	873:895	231 breast-milk samples	873:895	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	12	7	theme	unique	1986:1991	arg1	modifications					2000:2012	unique glycan modifications	1986:2012	unique glycan modifications that varied systematically in structure even with site specificity	1986:2079	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	13	8	from	role	2086:2089	arg1	proteins					2122:2129	human milk proteins	2111:2129	human milk proteins	2111:2129	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	7	9	dep	RESULTS	1141:1147	arg1	β-casein					1164:1171	β-casein	1164:1171	β-casein	1164:1171	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	9	dep	RESULTS	1141:1147	arg1	α-Lactalbumin					1149:1161	α-Lactalbumin	1149:1161	α-Lactalbumin	1149:1161	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	9	dep	RESULTS	1141:1147	arg1	RESULTS					1141:1147	RESULTS	1141:1147	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin	1141:1200	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	9	dep	RESULTS	1141:1147	arg1	k-casein					1174:1181	k-casein	1174:1181	k-casein	1174:1181	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	9	dep	RESULTS	1141:1147	arg1	α-antitrypsin					1188:1200	α-antitrypsin	1188:1200	α-antitrypsin	1188:1200	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	2	10	theme	protein	329:335	arg1	structure					337:345	protein structure	329:345	protein structure	329:345	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	12	11	theme	Individual	1953:1962	arg1	proteins					1969:1976	Individual milk proteins	1953:1976	Individual milk proteins	1953:1976	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	4	12	theme	site-specific	631:643	arg1	glycosylation					645:657	their site-specific glycosylation	625:657	their site-specific glycosylation	625:657	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	13	13	gly	glycosylation	2094:2106	arg1	milk					2117:2120	human milk proteins	2111:2129	human milk proteins	2111:2129	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	13	14	theme	milk	2117:2120	arg1	proteins					2122:2129	human milk proteins	2111:2129	human milk proteins	2111:2129	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	10	15	theme	A	1747:1747	arg1	glycans					1594:1600	Fucosylated glycans	1582:1600	Fucosylated glycans of lactoferrin	1582:1615	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	10	15	theme	A	1747:1747	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	6	16	theme	milk	1031:1034	arg1	proteins					1036:1043	milk proteins	1031:1043	milk proteins	1031:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	0	17	theme	Dynamic	65:71	arg1	Variations					73:82	Quantitative Dynamic Variations	52:82	Quantitative Dynamic Variations	52:82	Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.					
31098625	10	18	gly	fucosylated + sialylated	1686:1709	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	4	19	theme	large	688:692	arg1	cohort					694:699	a large cohort	686:699	a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation	686:814	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	4	20	gly	N-glycosylation	783:797	arg1	concentrations					744:757	individual protein concentrations	725:757	individual protein concentrations	725:757	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	4	21	from	N-glycosylation	783:797	arg1	concentrations					744:757	individual protein concentrations	725:757	individual protein concentrations	725:757	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	10	22	theme	proteins	1819:1826	arg1	concentrations					1790:1803	the concentrations	1786:1803	the concentrations of the parent proteins	1786:1826	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	10	23	gly	sialylated	1661:1670	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	7	24	dep	g/L	1299:1301	arg1	g/L					1392:1394	0.09 ± 0.10 g/L	1380:1394	0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L	1380:1413	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	24	dep	g/L	1299:1301	arg1	g/L					1316:1318	2.25 ± 0.86 g/L	1304:1318	2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L	1304:1337	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	24	dep	g/L	1299:1301	arg1	to					1284:1285	to	1284:1285	to	1284:1285	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	24	dep	g/L	1299:1301	arg1	g/L					1352:1354	1.33 ± 0.44 g/L	1340:1354	1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L	1340:1373	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	7	24	dep	g/L	1299:1301	arg1	P < 0.002					1430:1438	P < 0.002	1430:1438	P < 0.002	1430:1438	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	11	25	theme	infants	1944:1950	arg1	development					1929:1939	the development	1925:1939	the development of infants	1925:1950	CONCLUSIONS Proteins in healthy mothers vary dynamically through lactation to support the development of infants.					
31098625	10	26	gly	fucosylated	1648:1658	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	6	27	theme	site-specific	1070:1082	arg1	N-glycosylation					1084:1098	site-specific N-glycosylation	1070:1098	site-specific N-glycosylation of individual proteins	1070:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	4	28	theme	individual	725:734	arg1	concentrations					744:757	individual protein concentrations	725:757	individual protein concentrations	725:757	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	0	29	theme	Human	0:4	arg1	Proteins					11:18	Human Milk Proteins	0:18	Human Milk Proteins	0:18	Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.					
31098625	1	30	theme	BACKGROUND	102:111	arg1	Proteins					113:120	BACKGROUND Proteins	102:120	BACKGROUND Proteins in human milk	102:134	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	10	31	theme	parent	1812:1817	arg1	proteins					1819:1826	the parent proteins	1808:1826	the parent proteins	1808:1826	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	2	32	theme	intestinal	387:396	arg1	mucosa					398:403	the intestinal mucosa	383:403	the intestinal mucosa	383:403	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	5	33	theme	spectrometry	838:849	arg1	method					851:856	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	7	34	theme	g/L	1280:1282	arg1	g/L					1299:1301	4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L	1268:1301	4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L	1268:1301	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	8	35	theme	mature	1513:1518	arg1	milk					1520:1523	mature milk	1513:1523	mature milk	1513:1523	α-Lactalbumin (37%), β-casein (9%), and lysozyme (159%) were higher in mature milk than in colostrum.					
31098625	3	36	theme	quantitative	452:463	arg1	unknown					543:549	unknown	543:549	unknown	543:549	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	3	36	theme	quantitative	452:463	arg1	changes					465:471	the quantitative changes	448:471	the quantitative changes in milk proteins and their respective site-specific glycosylation	448:537	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	5	37	theme	DESIGN	817:822	arg1	method					851:856	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	7	38	dep	increased	1221:1229	arg1	g/L					1299:1301	4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L	1268:1301	4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L	1268:1301	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	13	39	theme	milk	2201:2204	arg1	components					2181:2190	the functional components	2166:2190	the functional components of human milk	2166:2204	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	4	40	theme	analytical	586:595	arg1	tools					597:601	the analytical tools	582:601	the analytical tools for milk proteins and their site-specific glycosylation	582:657	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	6	41	theme	proteins	1114:1121	arg1	N-glycosylation					1084:1098	site-specific N-glycosylation	1070:1098	site-specific N-glycosylation of individual proteins	1070:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	11	42	theme	healthy	1863:1869	arg1	mothers					1871:1877	healthy mothers	1863:1877	healthy mothers	1863:1877	CONCLUSIONS Proteins in healthy mothers vary dynamically through lactation to support the development of infants.					
31098625	13	43	theme	functional	2170:2179	arg1	components					2181:2190	the functional components	2166:2190	the functional components of human milk	2166:2204	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	6	44	from	variation	1057:1065	arg1	abundances					1017:1026	the absolute abundances	1004:1026	the absolute abundances of milk proteins	1004:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	6	44	from	variation	1057:1065	arg1	N-glycosylation					1084:1098	site-specific N-glycosylation	1070:1098	site-specific N-glycosylation of individual proteins	1070:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	3	45	theme	site-specific	511:523	arg1	glycosylation					525:537	their respective site-specific glycosylation	494:537	their respective site-specific glycosylation	494:537	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	10	46	theme	secretory	1722:1730	arg1	A					1747:1747	secretory immunoglobulin A	1722:1747	secretory immunoglobulin A	1722:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	6	47	theme	Dynamic	985:991	arg1	changes					993:999	Dynamic changes	985:999	Dynamic changes in the absolute abundances of milk proteins	985:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	3	48	from	changes	465:471	arg1	glycosylation					525:537	their respective site-specific glycosylation	494:537	their respective site-specific glycosylation	494:537	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	3	48	from	changes	465:471	arg1	proteins					481:488	milk proteins	476:488	milk proteins	476:488	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	12	49	theme	glycan	1993:1998	arg1	modifications					2000:2012	unique glycan modifications	1986:2012	unique glycan modifications that varied systematically in structure even with site specificity	1986:2079	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	10	50	theme	Fucosylated	1582:1592	arg1	glycans					1594:1600	Fucosylated glycans	1582:1600	Fucosylated glycans of lactoferrin	1582:1615	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	1	51	from	Proteins	113:120	arg1	milk					131:134	human milk	125:134	human milk	125:134	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	1	52	theme	human	125:129	arg1	milk					131:134	human milk	125:134	human milk	125:134	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	12	53	theme	milk	1964:1967	arg1	proteins					1969:1976	Individual milk proteins	1953:1976	Individual milk proteins	1953:1976	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	6	54	theme	proteins	1036:1043	arg1	abundances					1017:1026	the absolute abundances	1004:1026	the absolute abundances of milk proteins	1004:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	7	55	theme	transitional	1249:1260	arg1	milk					1262:1265	transitional milk	1249:1265	transitional milk	1249:1265	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	12	56	contain	carried	1978:1984	arg2	modifications					2000:2012	unique glycan modifications	1986:2012	unique glycan modifications that varied systematically in structure even with site specificity	1986:2079	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	12	56	contain	carried	1978:1984	arg1	proteins					1969:1976	Individual milk proteins	1953:1976	Individual milk proteins	1953:1976	Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity.					
31098625	13	57	theme	glycosylation	2094:2106	arg1	role					2086:2089	The role	2082:2089	The role of glycosylation in human milk proteins	2082:2129	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	13	57	theme	glycosylation	2094:2106	arg1	important					2139:2147	important	2139:2147	important	2139:2147	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	4	58	dep	OBJECTIVE	552:560	arg1	expanded					573:580	expanded	573:580	expanded the analytical tools for milk proteins and their site-specific glycosylation	573:657	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	4	58	dep	OBJECTIVE	552:560	arg1	applied					663:669	applied	663:669	applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation	663:814	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	6	59	theme	absolute	1008:1015	arg1	abundances					1017:1026	the absolute abundances	1004:1026	the absolute abundances of milk proteins	1004:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	13	60	theme	human	2111:2115	arg1	proteins					2122:2129	human milk proteins	2111:2129	human milk proteins	2111:2129	The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk.					
31098625	2	61	mod	modified	261:268	arg1	proteins					241:248	These proteins	235:248	These proteins	235:248	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	2	61	mod	modified	261:268	arg3	glycans					273:279	glycans	273:279	glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa	273:403	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	5	62	theme	lactation	974:982	arg1	periods					963:969	7 different periods	951:969	7 different periods of lactation	951:982	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	11	63	theme	CONCLUSIONS	1839:1849	arg1	Proteins					1851:1858	CONCLUSIONS Proteins	1839:1858	CONCLUSIONS Proteins in healthy mothers	1839:1877	CONCLUSIONS Proteins in healthy mothers vary dynamically through lactation to support the development of infants.					
31098625	5	64	from	mothers	905:911	arg1	California					923:932	California	923:932	California	923:932	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	5	64	from	mothers	905:911	arg1	samples					889:895	231 breast-milk samples	873:895	231 breast-milk samples	873:895	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	6	65	from	changes	993:999	arg1	abundances					1017:1026	the absolute abundances	1004:1026	the absolute abundances of milk proteins	1004:1043	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	6	65	from	changes	993:999	arg1	N-glycosylation					1084:1098	site-specific N-glycosylation	1070:1098	site-specific N-glycosylation of individual proteins	1070:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	4	66	theme	site-specific	769:781	arg1	N-glycosylation					783:797	their site-specific N-glycosylation	763:797	their site-specific N-glycosylation across lactation	763:814	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	10	67	theme	lactoferrin	1605:1615	arg1	glycans					1594:1600	Fucosylated glycans	1582:1600	Fucosylated glycans of lactoferrin	1582:1615	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	10	67	theme	lactoferrin	1605:1615	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	0	68	theme	Milk	6:9	arg1	Proteins					11:18	Human Milk Proteins	0:18	Human Milk Proteins	0:18	Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.					
31098625	11	69	from	Proteins	1851:1858	arg1	mothers					1871:1877	healthy mothers	1863:1877	healthy mothers	1863:1877	CONCLUSIONS Proteins in healthy mothers vary dynamically through lactation to support the development of infants.					
31098625	5	70	theme	mass	833:836	arg1	spectrometry					838:849	A tandem mass spectrometry	824:849	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	5	71	theme	tandem	826:831	arg1	spectrometry					838:849	A tandem mass spectrometry	824:849	DESIGN A tandem mass spectrometry method	817:856	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	5	72	theme	different	953:961	arg1	periods					963:969	7 different periods	951:969	7 different periods of lactation	951:982	DESIGN A tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation.					
31098625	2	73	theme	mucosa	398:403	arg1	structure					337:345	protein structure	329:345	protein structure	329:345	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	2	73	theme	mucosa	398:403	arg1	function					359:366	function	359:366	function	359:366	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	2	73	theme	mucosa	398:403	arg1	health					373:378	health	373:378	health	373:378	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	2	73	theme	mucosa	398:403	arg1	stability					348:356	stability	348:356	stability	348:356	These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa.					
31098625	4	74	theme	protein	736:742	arg1	concentrations					744:757	individual protein concentrations	725:757	individual protein concentrations	725:757	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	10	75	theme	undecorated	1635:1645	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	7	76	dep	milk	1262:1265	arg1	colostrum					1236:1244	colostrum	1236:1244	colostrum	1236:1244	RESULTS α-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002).					
31098625	10	77	theme	high-mannose	1621:1632	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	0	78	theme	Quantitative	52:63	arg1	Variations					73:82	Quantitative Dynamic Variations	52:82	Quantitative Dynamic Variations	52:82	Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.					
31098625	6	79	gly	N-glycosylation	1084:1098	arg1	proteins					1114:1121	individual proteins	1103:1121	individual proteins	1103:1121	Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.					
31098625	10	80	theme	sialylated	1661:1670	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	4	81	from	changes	714:720	arg1	concentrations					744:757	individual protein concentrations	725:757	individual protein concentrations	725:757	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	9	82	theme	different	1562:1570	arg1	behavior					1572:1579	different behavior	1562:1579	different behavior	1562:1579	Glycans exhibited different behavior.					
31098625	10	83	theme	fucosylated	1648:1658	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	10	84	theme	combined	1677:1684	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	3	85	theme	milk	415:418	arg1	proteins					420:427	milk proteins	415:427	milk proteins	415:427	Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.					
31098625	4	86	theme	milk	607:610	arg1	proteins					612:619	milk proteins	607:619	milk proteins	607:619	OBJECTIVE This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.					
31098625	10	87	theme	fucosylated + sialylated	1686:1709	arg1	glycans					1711:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans	1621:1717	high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A	1621:1747	Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.					
31098625	1	88	theme	newborn	226:232	arg1	protection					196:205	protection	196:205	protection	196:205	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	1	88	theme	newborn	226:232	arg1	health					212:217	health	212:217	health	212:217	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	1	88	theme	newborn	226:232	arg1	development					183:193	development	183:193	development	183:193	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
31098625	1	88	theme	newborn	226:232	arg1	growth					175:180	growth	175:180	growth	175:180	BACKGROUND Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn.					
30152909	2	0	theme	Lumos	778:782	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	1	theme	collisional	1312:1322	arg1	energies					1324:1331	low collisional energies	1308:1331	low collisional energies	1308:1331	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	2	theme	intact	582:587	arg1	glycoforms					589:598	its intact glycoforms	578:598	its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16)	578:673	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	3	theme	Orbitrap	762:769	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	6	4	theme	high	1814:1817	arg1	energies					1819:1826	low and high energies	1806:1826	low and high energies	1806:1826	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	1	5	theme	backbone	274:281	arg1	characterization					237:252	the structural characterization	222:252	the structural characterization of both the protein backbone and the glycan moiety	222:303	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	4	6	from	ions	1365:1368	arg1	EThcD					1299:1303	EThcD	1299:1303	EThcD at low collisional energies, but only a few matched product ions	1299:1368	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	7	theme	Mann	611:614	arg1	glycoforms					589:598	its intact glycoforms	578:598	its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16)	578:673	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	8	theme	few	1345:1347	arg1	ions					1365:1368	a few matched product ions	1343:1368	a few matched product ions	1343:1368	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	1	9	gly	glycoprotein	201:212	arg1	glycoprotein					201:212	an intact glycoprotein	191:212	an intact glycoprotein	191:212	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	10	theme	selective	461:469	arg1	fragmentation					471:483	selective fragmentation	461:483	selective fragmentation of the glycan moiety	461:504	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	11	theme	B	544:544	arg1	solution					546:553	METHODS RNase B solution	530:553	METHODS RNase B solution	530:553	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	12	theme	product	1357:1363	arg1	ions					1365:1368	a few matched product ions	1343:1368	a few matched product ions	1343:1368	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	13	theme	Lumos	108:112	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	1	14	theme	protein	388:394	arg1	backbone					396:403	the protein backbone	384:403	the protein backbone	384:403	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	15	theme	intact	194:199	arg1	glycoprotein					201:212	an intact glycoprotein	191:212	an intact glycoprotein	191:212	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	16	theme	METHODS	530:536	arg1	solution					546:553	METHODS RNase B solution	530:553	METHODS RNase B solution	530:553	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	0	17	theme	mass	122:125	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	4	18	from	energies	1324:1331	arg1	EThcD					1299:1303	EThcD	1299:1303	EThcD at low collisional energies, but only a few matched product ions	1299:1368	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	6	19	theme	tandem	1671:1676	arg1	spectrometry					1683:1694	tandem mass spectrometry	1671:1694	tandem mass spectrometry	1671:1694	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	20	theme	comprehensive	1390:1402	arg1	fragmentation					1404:1416	more comprehensive fragmentation	1385:1416	more comprehensive fragmentation	1385:1416	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	6	21	theme	N-glycoproteins	1649:1663	arg1	characterization					1622:1637	comprehensive structural characterization	1597:1637	comprehensive structural characterization of intact N-glycoproteins	1597:1663	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	22	theme	different	1125:1133	arg1	parameters					1148:1157	different dissociation parameters	1125:1157	different dissociation parameters for all the five methods	1125:1182	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	22	theme	different	1125:1133	arg1	fragmentation					1195:1207	selective fragmentation	1185:1207	selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1185:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	23	theme	backbone	59:66	arg1	fragmentation					10:22	Selective fragmentation	0:22	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.	0:139	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	4	24	theme	parameters	1148:1157	arg1	exploration					1110:1120	exploration	1110:1120	exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1110:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	25	theme	ribonuclease	71:82	arg1	B					84:84	ribonuclease B	71:84	ribonuclease B	71:84	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	3	26	theme	database	1030:1037	arg1	engines					1046:1052	the protein and N-glycan database search engines	1005:1052	engines	1046:1052	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	6	27	theme	intact	1642:1647	arg1	N-glycoproteins					1649:1663	intact N-glycoproteins	1642:1663	intact N-glycoproteins	1642:1663	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	1	28	theme	functional	155:164	arg1	study					166:170	The functional study	151:170	RATIONALE The functional study	141:170	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	29	theme	protein	909:915	arg1	backbone					917:924	protein backbone	909:924	protein backbone	909:924	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	30	from	EThcD	749:753	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	1	31	theme	selective	357:365	arg1	fragmentation					367:379	selective fragmentation	357:379	selective fragmentation of the protein backbone in CID and ExD	357:418	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	32	theme	sequence	942:949	arg1	coverage					951:958	high sequence coverage	937:958	high sequence coverage	937:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	33	theme	moiety	1225:1230	arg1	fragmentation					1195:1207	selective fragmentation	1185:1207	selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1185:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	33	theme	moiety	1225:1230	arg1	parameters					1148:1157	different dissociation parameters	1125:1157	different dissociation parameters for all the five methods	1125:1182	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	34	theme	Orbitrap	92:99	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	6	35	theme	comprehensive	1597:1609	arg1	characterization					1622:1637	comprehensive structural characterization	1597:1637	comprehensive structural characterization of intact N-glycoproteins	1597:1663	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	36	theme	charge	654:659	arg1	abundance					643:651	the highest abundance	631:651	the highest abundance (charge state z = 16)	631:673	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	36	theme	charge	654:659	arg1	state					661:665	charge state z = 16	654:672	charge state z = 16	654:672	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	6	37	theme	optimal	1940:1946	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	38	theme	protein	1465:1471	arg1	backbone					1473:1480	the protein backbone	1461:1480	the protein backbone lost	1461:1485	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	39	theme	Selective	0:8	arg1	fragmentation					10:22	Selective fragmentation	0:22	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.	0:139	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	5	40	theme	Selective	1489:1497	arg1	fragmentation					1516:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	1	41	from	fragmentation	367:379	arg1	ExD					416:418	ExD	416:418	ExD	416:418	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	41	from	fragmentation	367:379	arg1	CID					408:410	CID	408:410	CID	408:410	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	42	theme	glycan	291:296	arg1	moiety					298:303	the glycan moiety	287:303	the glycan moiety	287:303	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	6	43	theme	moiety	1742:1747	arg1	topology					1717:1724	topology	1717:1724	topology	1717:1724	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	6	43	theme	moiety	1742:1747	arg1	composition					1701:1711	composition	1701:1711	composition	1701:1711	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	5	44	theme	backbone	1507:1514	arg1	fragmentation					1516:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	4	45	theme	collisional	1439:1449	arg1	energies					1451:1458	high collisional energies	1434:1458	high collisional energies (the protein backbone lost)	1434:1486	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	46	theme	N-glycan	31:38	arg1	moiety					40:45	N-glycan moiety	31:45	N-glycan moiety	31:45	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	4	47	with	RESULTS	1097:1103	arg1	exploration					1110:1120	exploration	1110:1120	exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1110:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	48	theme	moiety	898:903	arg1	fragmentation					868:880	selective fragmentation	858:880	selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage	858:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	6	49	with	EThcD	1923:1927	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	1	50	theme	moiety	499:504	arg1	fragmentation					471:483	selective fragmentation	461:483	selective fragmentation of the glycan moiety	461:504	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	6	51	theme	amino	1839:1843	arg1	sequence					1850:1857	the amino acid sequence	1835:1857	the amino acid sequence	1835:1857	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	52	theme	Tribrid	784:790	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	53	theme	mass	792:795	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	5	54	theme	dissociation	1559:1570	arg1	methods					1572:1578	all the five dissociation methods	1546:1578	all the five dissociation methods	1546:1578	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	4	55	dep	moiety	1225:1230	arg1	backbone					1245:1252	the protein backbone	1233:1252	the protein backbone staying intact	1233:1267	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	56	dep	electrosprayed	559:572	arg1	optimized					844:852	optimized	844:852	were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage	839:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	3	57	theme	obtained	965:972	arg1	spectra					974:980	The obtained spectra	961:980	The obtained spectra	961:980	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	2	58	theme	Fusion	771:776	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	59	theme	low	1308:1310	arg1	energies					1324:1331	low collisional energies	1308:1331	low collisional energies	1308:1331	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	1	60	theme	protein	266:272	arg1	backbone					274:281	the protein backbone	262:281	the protein backbone	262:281	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	61	theme	dissociation	815:826	arg1	parameters					828:837	the dissociation parameters	811:837	the dissociation parameters	811:837	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	6	62	gly	N-glycoproteins	1649:1663	arg1	N-glycoproteins					1649:1663	intact N-glycoproteins	1642:1663	intact N-glycoproteins	1642:1663	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	63	with	glycoforms	589:598	arg1	abundance					643:651	the highest abundance	631:651	the highest abundance (charge state z = 16)	631:673	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	63	with	glycoforms	589:598	arg1	state					661:665	charge state z = 16	654:672	charge state z = 16	654:672	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	64	theme	backbone	917:924	arg1	fragmentation					868:880	selective fragmentation	858:880	selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage	858:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	65	theme	GlcNAc2	603:609	arg1	Mann					611:614	GlcNAc2 Mann	603:614	GlcNAc2 Mann (n = 5-9)	603:624	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	65	theme	GlcNAc2	603:609	arg1	n = 5-9					617:623	n = 5-9	617:623	n = 5-9	617:623	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	66	located	observed	1422:1429	arg2	fragmentation					1404:1416	more comprehensive fragmentation	1385:1416	more comprehensive fragmentation	1385:1416	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	66	located	observed	1422:1429	arg2	RESULTS					1097:1103	RESULTS	1097:1103	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1097:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	66	located	observed	1422:1429	arg1	energies					1451:1458	high collisional energies	1434:1458	high collisional energies (the protein backbone lost)	1434:1486	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	67	theme	Tribrid	114:120	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	4	68	theme	N-glycan	1216:1223	arg1	moiety					1225:1230	the N-glycan moiety	1212:1230	the N-glycan moiety (the protein backbone staying intact)	1212:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	69	gly	glycoforms	589:598	arg1	Mann					611:614	GlcNAc2 Mann	603:614	GlcNAc2 Mann (n = 5-9)	603:624	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	69	gly	glycoforms	589:598	arg1	n = 5-9					617:623	n = 5-9	617:623	n = 5-9	617:623	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	70	theme	RNase	538:542	arg1	solution					546:553	METHODS RNase B solution	530:553	METHODS RNase B solution	530:553	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	71	theme	matched	1349:1355	arg1	ions					1365:1368	a few matched product ions	1343:1368	a few matched product ions	1343:1368	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	1	72	theme	backbone	396:403	arg1	fragmentation					367:379	selective fragmentation	357:379	selective fragmentation of the protein backbone in CID and ExD	357:418	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	73	theme	glycoprotein	201:212	arg1	application					176:186	application	176:186	application of an intact glycoprotein	176:212	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	1	73	theme	glycoprotein	201:212	arg1	RATIONALE					141:149	RATIONALE	141:149	RATIONALE The functional study	141:170	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	6	74	with	CID	1897:1899	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	6	75	theme	N-glycan	1733:1740	arg1	moiety					1742:1747	the N-glycan moiety	1729:1747	the N-glycan moiety	1729:1747	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	6	76	dep	composition	1701:1711	arg1	the					1697:1699	the	1697:1699	the	1697:1699	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	6	77	gly	glycosite	1863:1871	arg2	glycosite					1863:1871	glycosite	1863:1871	glycosite	1863:1871	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	6	78	theme	mass	1678:1681	arg1	spectrometry					1683:1694	tandem mass spectrometry	1671:1694	tandem mass spectrometry	1671:1694	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	79	theme	dissociation	1135:1146	arg1	parameters					1148:1157	different dissociation parameters	1125:1157	different dissociation parameters for all the five methods	1125:1182	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	79	theme	dissociation	1135:1146	arg1	fragmentation					1195:1207	selective fragmentation	1185:1207	selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1185:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	6	80	with	ETciD	1912:1916	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	81	dep	energies	1451:1458	arg1	backbone					1473:1480	the protein backbone	1461:1480	the protein backbone lost	1461:1485	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	82	theme	B	84:84	arg1	moiety					40:45	N-glycan moiety	31:45	N-glycan moiety	31:45	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	0	82	theme	B	84:84	arg1	backbone					59:66	protein backbone	51:66	protein backbone	51:66	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	3	83	theme	N-glycan	1021:1028	arg1	engines					1046:1052	the protein and N-glycan database search engines	1005:1052	engines	1046:1052	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	6	84	with	ETD	1902:1904	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	85	dep	observed	1274:1281	arg1	observed					1422:1429	observed	1422:1429	was observed at high collisional energies (the protein backbone lost)	1418:1486	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	86	from	fragmentation	10:22	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	2	87	theme	highest	635:641	arg1	abundance					643:651	the highest abundance	631:651	the highest abundance (charge state z = 16)	631:673	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	87	theme	highest	635:641	arg1	state					661:665	charge state z = 16	654:672	charge state z = 16	654:672	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	2	88	theme	coverage	951:958	arg1	fragmentation					868:880	selective fragmentation	858:880	selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage	858:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	0	89	theme	Fusion	101:106	arg1	spectrometer					127:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	an Orbitrap Fusion Lumos Tribrid mass spectrometer	89:138	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	6	90	theme	structural	1611:1620	arg1	characterization					1622:1637	comprehensive structural characterization	1597:1637	comprehensive structural characterization of intact N-glycoproteins	1597:1663	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	91	theme	high	937:940	arg1	coverage					951:958	high sequence coverage	937:958	high sequence coverage	937:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	92	theme	selective	1185:1193	arg1	fragmentation					1195:1207	selective fragmentation	1185:1207	selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1185:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	92	theme	selective	1185:1193	arg1	parameters					1148:1157	different dissociation parameters	1125:1157	different dissociation parameters for all the five methods	1125:1182	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	6	93	theme	dissociation	1948:1959	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	3	94	dep	protein	1009:1015	arg1	GlySeeker					1072:1080	GlySeeker	1072:1080	GlySeeker	1072:1080	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	3	94	dep	protein	1009:1015	arg1	ProteinGoggle					1054:1066	ProteinGoggle	1054:1066	ProteinGoggle	1054:1066	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	3	95	theme	search	1039:1044	arg1	engines					1046:1052	the protein and N-glycan database search engines	1005:1052	engines	1046:1052	The obtained spectra were interpreted using the protein and N-glycan database search engines ProteinGoggle and GlySeeker, respectively.					
30152909	6	96	theme	low	1806:1808	arg1	energies					1819:1826	low and high energies	1806:1826	low and high energies	1806:1826	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	4	97	located	observed	1274:1281	arg1	HCD					1291:1293	both HCD	1286:1293	both HCD	1286:1293	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	4	97	located	observed	1274:1281	arg2	RESULTS					1097:1103	RESULTS	1097:1103	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact)	1097:1268	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	98	theme	selective	858:866	arg1	fragmentation					868:880	selective fragmentation	858:880	selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage	858:958	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	5	99	theme	protein	1499:1505	arg1	fragmentation					1516:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	1	100	dep	RATIONALE	141:149	arg1	study					166:170	The functional study	151:170	RATIONALE The functional study	141:170	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	0	101	theme	moiety	40:45	arg1	fragmentation					10:22	Selective fragmentation	0:22	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.	0:139	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	1	102	theme	moiety	298:303	arg1	characterization					237:252	the structural characterization	222:252	the structural characterization of both the protein backbone and the glycan moiety	222:303	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	103	from	CID	723:725	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	104	theme	high	1434:1437	arg1	energies					1451:1458	high collisional energies	1434:1458	high collisional energies (the protein backbone lost)	1434:1486	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	0	105	theme	protein	51:57	arg1	backbone					59:66	protein backbone	51:66	protein backbone	51:66	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	0	106	dep	moiety	40:45	arg1	the					27:29	the	27:29	the	27:29	Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.					
30152909	6	107	with	HCD	1907:1909	arg1	parameters					1961:1970	their optimal dissociation parameters	1934:1970	their optimal dissociation parameters	1934:1970	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	108	theme	N-glycan	889:896	arg1	moiety					898:903	the N-glycan moiety	885:903	the N-glycan moiety	885:903	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	4	109	theme	protein	1237:1243	arg1	backbone					1245:1252	the protein backbone	1233:1252	the protein backbone staying intact	1233:1267	RESULTS With exploration of different dissociation parameters for all the five methods, selective fragmentation of the N-glycan moiety (the protein backbone staying intact) was observed in both HCD and EThcD at low collisional energies, but only a few matched product ions were observed; more comprehensive fragmentation was observed at high collisional energies (the protein backbone lost).					
30152909	2	110	from	ETD	728:730	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	1	111	theme	glycan	492:497	arg1	moiety					499:504	the glycan moiety	488:504	the glycan moiety	488:504	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	6	112	theme	acid	1845:1848	arg1	sequence					1850:1857	the amino acid sequence	1835:1857	the amino acid sequence	1835:1857	CONCLUSIONS For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.					
30152909	2	113	from	HCD	733:735	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
30152909	5	114	located	observed	1534:1541	arg2	fragmentation					1516:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation	1489:1528	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	5	114	located	observed	1534:1541	arg1	methods					1572:1578	all the five dissociation methods	1546:1578	all the five dissociation methods	1546:1578	Selective protein backbone fragmentation was observed in all the five dissociation methods.					
30152909	1	115	theme	structural	226:235	arg1	characterization					237:252	the structural characterization	222:252	the structural characterization of both the protein backbone and the glycan moiety	222:303	RATIONALE The functional study and application of an intact glycoprotein require the structural characterization of both the protein backbone and the glycan moiety; the former has been successfully demonstrated with selective fragmentation of the protein backbone in CID and ExD; whether the latter can be achieved with selective fragmentation of the glycan moiety remains to be explored.					
30152909	2	116	from	ETciD	738:742	arg1	spectrometer					797:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	the Orbitrap Fusion Lumos Tribrid mass spectrometer	758:808	METHODS RNase B solution was electrosprayed and its intact glycoforms of GlcNAc2 Mann (n = 5-9) with the highest abundance (charge state z = 16) were isolated individually and fragmented using CID, ETD, HCD, ETciD, and EThcD on the Orbitrap Fusion Lumos Tribrid mass spectrometer; the dissociation parameters were optimized for selective fragmentation of the N-glycan moiety and protein backbone as well as high sequence coverage.					
31738061	4	0	theme	collective	680:689	arg1	synthesis					691:699	the first collective synthesis	670:699	the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns	670:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	1	1	theme	unique	144:149	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	1	theme	unique	144:149	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	5	2	theme	sulfated	930:937	arg1	branch					945:950	sulfated HNK-1 branch and substrate promiscuities	930:978	branch	945:950	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	6	3	theme	antibodies	1336:1345	arg1	roles					1365:1369	the essential roles	1351:1369	the essential roles of the sulfate group	1351:1390	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	3	theme	antibodies	1336:1345	arg1	epitopes					1318:1325	the recognition epitopes	1302:1325	the recognition epitopes of these antibodies	1302:1345	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	1	4	gly	glycoproteins	224:236	arg1	glycoproteins					224:236	various glycoproteins	216:236	various glycoproteins	216:236	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	5	theme	sulfated	151:158	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	5	theme	sulfated	151:158	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	3	6	from	investigation	404:416	arg1	context					472:478	a more complex glycan context	450:478	a more complex glycan context	450:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	6	7	theme	recognition	1306:1316	arg1	epitopes					1318:1325	the recognition epitopes	1302:1325	the recognition epitopes of these antibodies	1302:1345	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	2	8	theme	nervous	285:291	arg1	system					293:298	the nervous system	281:298	the nervous system	281:298	It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases.					
31738061	4	9	theme	efficient	632:640	arg1	approach					657:664	a highly efficient chemoenzymatic approach	623:664	a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts	623:808	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	5	10	theme	successful	815:824	arg1	strategy					826:833	The successful strategy	811:833	The successful strategy	811:833	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	3	11	theme	due	527:529	arg1	challenge					517:525	a big challenge	511:525	a big challenge due to lack of access to related structurally well-defined complex glycans	511:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	3	12	from	level	497:501	arg1	investigation					404:416	the investigation	400:416	the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level	400:501	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	2	13	theme	synaptic	347:354	arg1	plasticity					356:365	synaptic plasticity	347:365	synaptic plasticity	347:365	It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases.					
31738061	4	14	theme	HNK-1-bearing	704:716	arg1	glycans					728:734	HNK-1-bearing O-mannose glycans	704:734	HNK-1-bearing O-mannose glycans with different branching patterns	704:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	4	15	theme	first	674:678	arg1	synthesis					691:699	the first collective synthesis	670:699	the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns	670:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	6	16	theme	glycan-antibody	1402:1416	arg1	recognition					1418:1428	HNK-1 glycan-antibody recognition	1396:1428	HNK-1 glycan-antibody recognition	1396:1428	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	5	17	theme	HNK-1	939:943	arg1	branch					945:950	sulfated HNK-1 branch and substrate promiscuities	930:978	branch	945:950	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	1	18	theme	trisaccharide	160:172	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	18	theme	trisaccharide	160:172	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	6	19	theme	HNK-1-specific	1193:1206	arg1	Cat-315					1257:1263	Cat-315	1257:1263	Cat-315	1257:1263	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	19	theme	HNK-1-specific	1193:1206	arg1	anti-HNK-1/N-CAM					1229:1244	anti-HNK-1/N-CAM	1229:1244	anti-HNK-1/N-CAM (CD57)	1229:1251	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	19	theme	HNK-1-specific	1193:1206	arg1	antibodies					1208:1217	two HNK-1-specific antibodies	1189:1217	two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition	1189:1428	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	3	20	theme	big	513:515	arg1	challenge					517:525	a big challenge	511:525	a big challenge due to lack of access to related structurally well-defined complex glycans	511:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	6	21	theme	synthetic	1137:1145	arg1	glycans					1147:1153	the resulting complex synthetic glycans	1115:1153	the resulting complex synthetic glycans	1115:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	5	22	theme	sulfated	1032:1039	arg1	substrates					1041:1050	sulfated substrates	1032:1050	sulfated substrates for enzymatic diversification	1032:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	5	23	gly	glycosylation	859:871	arg1	donor					900:904	a trisaccharide lactone donor	876:904	a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	876:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	2	24	theme	crucial	310:316	arg1	roles					318:322	crucial roles	310:322	crucial roles	310:322	It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases.					
31738061	6	25	theme	complex	1129:1135	arg1	glycans					1147:1153	the resulting complex synthetic glycans	1115:1153	the resulting complex synthetic glycans	1115:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	2	26	theme	neuronal	372:379	arg1	diseases					381:388	neuronal diseases	372:388	neuronal diseases	372:388	It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases.					
31738061	0	27	theme	Chemoenzymatic	0:13	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of O-Mannose Glycans	0:44	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	6	28	theme	further	1281:1287	arg1	evidence					1289:1296	further evidence	1281:1296	further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition	1281:1428	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	3	29	from	context	472:478	arg1	investigation					404:416	the investigation	400:416	the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level	400:501	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	1	30	theme	human	100:104	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	30	theme	human	100:104	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	3	31	theme	functional	421:430	arg1	roles					432:436	functional roles	421:436	functional roles of HNK-1 in a more complex glycan context	421:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	0	32	theme	O-Mannose	28:36	arg1	Glycans					38:44	O-Mannose Glycans	28:44	O-Mannose Glycans	28:44	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	4	33	theme	glycans	728:734	arg1	synthesis					691:699	the first collective synthesis	670:699	the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns	670:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	5	34	theme	branch	945:950	arg1	introduction					914:925	the introduction	910:925	the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	910:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	1	35	theme	natural	106:112	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	35	theme	natural	106:112	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	5	36	theme	bacterial	983:991	arg1	glycosyltransferases					993:1012	bacterial glycosyltransferases	983:1012	bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	983:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	3	37	theme	access	542:547	arg1	lack					534:537	lack	534:537	lack of access to related structurally well-defined complex glycans	534:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	5	38	theme	substrate	956:964	arg1	promiscuities					966:978	sulfated HNK-1 branch and substrate promiscuities	930:978	promiscuities	966:978	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	5	39	theme	promiscuities	966:978	arg1	introduction					914:925	the introduction	910:925	the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	910:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	1	40	theme	killer-1	114:121	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	40	theme	killer-1	114:121	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	2	41	theme	nerve	327:331	arg1	regeneration					333:344	nerve regeneration	327:344	nerve regeneration	327:344	It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases.					
31738061	5	42	theme	chemical	850:857	arg1	glycosylation					859:871	both chemical glycosylation	845:871	both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	845:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	5	43	theme	enzymatic	1056:1064	arg1	diversification					1066:1080	enzymatic diversification	1056:1080	enzymatic diversification	1056:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	4	44	theme	chemoenzymatic	642:655	arg1	approach					657:664	a highly efficient chemoenzymatic approach	623:664	a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts	623:808	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	3	45	theme	HNK-1	441:445	arg1	roles					432:436	functional roles	421:436	functional roles of HNK-1 in a more complex glycan context	421:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	0	46	theme	Glycans	38:44	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of O-Mannose Glycans	0:44	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	5	47	theme	trisaccharide	878:890	arg1	donor					900:904	a trisaccharide lactone donor	876:904	a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	876:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	3	48	theme	well-defined	573:584	arg1	glycans					594:600	related structurally well-defined complex glycans	552:600	related structurally well-defined complex glycans	552:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	6	49	theme	microarray	1090:1099	arg1	analysis					1101:1108	Glycan microarray analysis	1083:1108	Glycan microarray analysis with the resulting complex synthetic glycans	1083:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	50	theme	HNK-1	1396:1400	arg1	recognition					1418:1428	HNK-1 glycan-antibody recognition	1396:1428	HNK-1 glycan-antibody recognition	1396:1428	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	3	51	theme	roles	432:436	arg1	investigation					404:416	the investigation	400:416	the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level	400:501	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	5	52	theme	glycosyltransferases	993:1012	arg1	introduction					914:925	the introduction	910:925	the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	910:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	0	53	theme	Sulfated	57:64	arg1	Epitope					87:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	6	54	theme	essential	1355:1363	arg1	roles					1365:1369	the essential roles	1351:1369	the essential roles of the sulfate group	1351:1390	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	55	theme	resulting	1119:1127	arg1	glycans					1147:1153	the resulting complex synthetic glycans	1115:1153	the resulting complex synthetic glycans	1115:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	56	with	analysis	1101:1108	arg1	glycans					1147:1153	the resulting complex synthetic glycans	1115:1153	the resulting complex synthetic glycans	1115:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	3	57	theme	related	552:558	arg1	glycans					594:600	related structurally well-defined complex glycans	552:600	related structurally well-defined complex glycans	552:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	3	58	theme	molecular	487:495	arg1	level					497:501	the molecular level	483:501	the molecular level	483:501	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	1	59	theme	HNK-1	124:128	arg1	epitope					131:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope	96:137	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	1	59	theme	HNK-1	124:128	arg1	sequence					174:181	a unique sulfated trisaccharide sequence	142:181	a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids	142:255	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	4	60	with	glycans	728:734	arg1	patterns					761:768	different branching patterns	741:768	different branching patterns	741:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	6	61	theme	group	1386:1390	arg1	roles					1365:1369	the essential roles	1351:1369	the essential roles of the sulfate group	1351:1390	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	6	61	theme	group	1386:1390	arg1	epitopes					1318:1325	the recognition epitopes	1302:1325	the recognition epitopes of these antibodies	1302:1345	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	5	62	theme	lactone	892:898	arg1	donor					900:904	a trisaccharide lactone donor	876:904	a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	876:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	4	63	theme	nonsulfated	785:795	arg1	counterparts					797:808	their nonsulfated counterparts	779:808	their nonsulfated counterparts	779:808	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	3	64	theme	complex	457:463	arg1	context					472:478	a more complex glycan context	450:478	a more complex glycan context	450:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	4	65	theme	branching	751:759	arg1	patterns					761:768	different branching patterns	741:768	different branching patterns	741:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	1	66	theme	various	216:222	arg1	glycoproteins					224:236	various glycoproteins	216:236	various glycoproteins	216:236	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	6	67	theme	sulfate	1378:1384	arg1	group					1386:1390	the sulfate group	1374:1390	the sulfate group	1374:1390	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31738061	4	68	theme	O-mannose	718:726	arg1	glycans					728:734	HNK-1-bearing O-mannose glycans	704:734	HNK-1-bearing O-mannose glycans with different branching patterns	704:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	4	69	theme	different	741:749	arg1	patterns					761:768	different branching patterns	741:768	different branching patterns	741:768	Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts.					
31738061	3	70	theme	glycan	465:470	arg1	context					472:478	a more complex glycan context	450:478	a more complex glycan context	450:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	1	71	theme	glycoproteins	224:236	arg1	O-					196:197	O-	196:197	O-	196:197	The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids.					
31738061	0	72	theme	HNK-1	81:85	arg1	Epitope					87:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	3	73	theme	complex	586:592	arg1	glycans					594:600	related structurally well-defined complex glycans	552:600	related structurally well-defined complex glycans	552:600	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	5	74	theme	donor	900:904	arg1	glycosylation					859:871	both chemical glycosylation	845:871	both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification	845:1080	The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification.					
31738061	0	75	theme	Nonsulfated	69:79	arg1	Epitope					87:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Sulfated or Nonsulfated HNK-1 Epitope	57:93	Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.					
31738061	3	76	from	roles	432:436	arg1	context					472:478	a more complex glycan context	450:478	a more complex glycan context	450:478	However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans.					
31738061	6	77	theme	Glycan	1083:1088	arg1	analysis					1101:1108	Glycan microarray analysis	1083:1108	Glycan microarray analysis with the resulting complex synthetic glycans	1083:1153	Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.					
31769974	4	0	theme	modeling	808:815	arg1	techniques					817:826	molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics	798:916	techniques	817:826	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	1	1	link	Lipid-linked	68:79	arg1	LLOs					99:102	LLOs	99:102	LLOs	99:102	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	1	1	link	Lipid-linked	68:79	arg1	oligosaccharides					81:96	Lipid-linked oligosaccharides	68:96	Lipid-linked oligosaccharides (LLOs)	68:103	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	4	2	dep	eukaryotic	941:950	arg1	Glc3-Man9-GlcNAc2-PP-Dolichol					953:981	Glc3-Man9-GlcNAc2-PP-Dolichol	953:981	Glc3-Man9-GlcNAc2-PP-Dolichol	953:981	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	1	3	theme	en	250:251	arg1	bloc					253:256	the en bloc	246:256	the en bloc transfer of glycan chains onto a nascent polypeptide	246:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	3	4	theme	further	669:675	arg1	studies					677:683	further studies	669:683	further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems	669:781	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	2	5	from	component	322:330	arg1	archaea					359:365	archaea	359:365	archaea	359:365	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	2	5	from	component	322:330	arg1	eukarya					347:353	eukarya	347:353	eukarya	347:353	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	8	6	from	studies	1753:1759	arg1	mechanism					1781:1789	the transferring mechanism	1764:1789	the transferring mechanism of the oligosaccharide chain to an acceptor protein	1764:1841	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	3	7	from	studies	677:683	arg1	formation					696:704	complex formation	688:704	complex formation	688:704	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	3	7	from	studies	677:683	arg1	processing					716:725	their processing	710:725	their processing by OSTs, as well as protein engineering on such systems	710:781	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	3	8	theme	LLOs	522:525	arg1	relevance					509:517	the potential relevance	495:517	the potential relevance of LLOs and their related enzymes to diverse biotechnological applications	495:592	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	1	9	theme	oligosaccharyltransferases	185:210	arg1	substrate					172:180	the donor substrate	162:180	the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide	162:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	4	10	theme	dynamics	879:886	arg1	simulations					888:898	molecular dynamics simulations	869:898	molecular dynamics simulations	869:898	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	3	11	theme	enzymes	545:551	arg1	relevance					509:517	the potential relevance	495:517	the potential relevance of LLOs and their related enzymes to diverse biotechnological applications	495:592	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	7	12	dep	structure	1563:1571	arg1	the					1559:1561	the	1559:1561	the	1559:1561	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	4	13	theme	mechanics	845:853	arg1	calculations					855:866	quantum mechanics calculations	837:866	quantum mechanics calculations	837:866	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	1	14	theme	bloc	253:256	arg1	transfer					258:265	the en bloc transfer	246:265	the en bloc transfer of glycan chains onto a nascent polypeptide	246:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	3	15	theme	protein	747:753	arg1	engineering					755:765	protein engineering	747:765	protein engineering	747:765	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	2	16	theme	isoprene	446:453	arg1	units					455:459	isoprene units	446:459	isoprene units	446:459	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	5	17	theme	PP	1303:1304	arg1	positioned					1319:1328	positioned	1319:1328	positioned	1319:1328	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	17	theme	PP	1303:1304	arg1	linkages					1306:1313	the PP linkages	1299:1313	the PP linkages	1299:1313	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	6	18	theme	isoprenoid	1388:1397	arg1	chains					1399:1404	isoprenoid chains	1388:1404	isoprenoid chains embedded within the bilayer	1388:1432	The dynamics of isoprenoid chains embedded within the bilayer are described, and membrane dynamics and related properties are also investigated.					
31769974	5	19	theme	Microsecond	1120:1130	arg1	simulations					1151:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	3	20	theme	diverse	556:562	arg1	applications					581:592	diverse biotechnological applications	556:592	diverse biotechnological applications	556:592	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	7	21	theme	eukaryotic	1593:1602	arg1	dynamics					1577:1584	dynamics	1577:1584	dynamics	1577:1584	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	7	21	theme	eukaryotic	1593:1602	arg1	structure					1563:1571	structure	1563:1571	structure	1563:1571	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	0	22	from	Life	9:12	arg1	Domains					59:65	All Life Domains	50:65	All Life Domains	50:65	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains.					
31769974	5	23	theme	lipid	1274:1278	arg1	groups					1285:1290	the membrane lipid head groups	1261:1290	the membrane lipid head groups	1261:1290	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	24	theme	dynamics	1142:1149	arg1	simulations					1151:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	4	25	dep	archaeal	1036:1043	arg1	Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol					1046:1090	Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol	1046:1090	Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol	1046:1090	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	7	26	theme	association	1700:1710	arg1	comprehension					1677:1689	the comprehension	1673:1689	the comprehension of their association with OSTs	1673:1720	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	3	27	from	formation	696:704	arg1	systems					775:781	such systems	770:781	such systems	770:781	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	5	28	theme	lipid	1337:1341	arg1	groups					1358:1363	lipid phosphate head groups	1337:1363	the lipid phosphate head groups level	1333:1369	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	1	29	theme	Lipid-linked	68:79	arg1	LLOs					99:102	LLOs	99:102	LLOs	99:102	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	1	29	theme	Lipid-linked	68:79	arg1	oligosaccharides					81:96	Lipid-linked oligosaccharides	68:96	Lipid-linked oligosaccharides (LLOs)	68:103	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	4	30	theme	bacterial	985:993	arg1	LLOs					1093:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	1	31	theme	glycan	270:275	arg1	chains					277:282	glycan chains	270:282	glycan chains	270:282	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	5	32	theme	head	1353:1356	arg1	groups					1358:1363	lipid phosphate head groups	1337:1363	the lipid phosphate head groups level	1333:1369	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	33	theme	LLOs	1196:1199	arg1	calculations					1180:1191	metadynamics calculations	1167:1191	metadynamics calculations	1167:1191	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	33	theme	LLOs	1196:1199	arg1	simulations					1151:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	34	from	level	1365:1369	arg1	positioned					1319:1328	positioned	1319:1328	positioned	1319:1328	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	34	from	level	1365:1369	arg1	linkages					1306:1313	the PP linkages	1299:1313	the PP linkages	1299:1313	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	3	35	theme	LLO	614:616	arg1	models					618:623	reliable LLO models	605:623	reliable LLO models	605:623	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	0	36	theme	Lipid-Linked	17:28	arg1	Oligosaccharides					30:45	Lipid-Linked Oligosaccharides	17:45	Lipid-Linked Oligosaccharides	17:45	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains.					
31769974	1	37	theme	chains	277:282	arg1	transfer					258:265	the en bloc transfer	246:265	the en bloc transfer of glycan chains onto a nascent polypeptide	246:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	0	38	theme	Life	54:57	arg1	Domains					59:65	All Life Domains	50:65	All Life Domains	50:65	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains.					
31769974	2	39	dep	dolichol	381:388	arg1	a					379:379	a	379:379	a	379:379	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	2	40	theme	lipid	316:320	arg1	component					322:330	The lipid component	312:330	The lipid component of LLO in both eukarya and archaea	312:365	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	6	41	theme	embedded	1406:1413	arg1	chains					1399:1404	isoprenoid chains	1388:1404	isoprenoid chains embedded within the bilayer	1388:1432	The dynamics of isoprenoid chains embedded within the bilayer are described, and membrane dynamics and related properties are also investigated.					
31769974	2	42	from	dolichol	381:388	arg1	prokarya					414:421	prokarya	414:421	prokarya	414:421	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	4	43	theme	molecular	798:806	arg1	techniques					817:826	molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics	798:916	techniques	817:826	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	4	44	theme	archaeal	1036:1043	arg1	LLOs					1093:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	3	45	theme	life	650:653	arg1	life					650:653	life	650:653	life	650:653	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	3	45	theme	life	650:653	arg1	domains					639:645	distinct domains	630:645	distinct domains of life	630:653	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	6	46	theme	membrane	1453:1460	arg1	dynamics					1462:1469	membrane dynamics	1453:1469	membrane dynamics	1453:1469	The dynamics of isoprenoid chains embedded within the bilayer are described, and membrane dynamics and related properties are also investigated.					
31769974	3	47	theme	complex	688:694	arg1	formation					696:704	complex formation	688:704	complex formation	688:704	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	4	48	dep	bacterial	985:993	arg1	Glc1-GalNAc5-Bac1-PP-Undecaprenol					996:1028	Glc1-GalNAc5-Bac1-PP-Undecaprenol	996:1028	Glc1-GalNAc5-Bac1-PP-Undecaprenol	996:1028	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	1	49	theme	donor	166:170	arg1	substrate					172:180	the donor substrate	162:180	the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide	162:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	4	50	theme	membrane	1101:1108	arg1	bilayers					1110:1117	membrane bilayers	1101:1117	membrane bilayers	1101:1117	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	3	51	theme	potential	499:507	arg1	relevance					509:517	the potential relevance	495:517	the potential relevance of LLOs and their related enzymes to diverse biotechnological applications	495:592	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	8	52	theme	chain	1814:1818	arg1	mechanism					1781:1789	the transferring mechanism	1764:1789	the transferring mechanism of the oligosaccharide chain to an acceptor protein	1764:1841	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	7	53	theme	archaeal	1628:1635	arg1	dynamics					1577:1584	dynamics	1577:1584	dynamics	1577:1584	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	7	53	theme	archaeal	1628:1635	arg1	structure					1563:1571	structure	1563:1571	structure	1563:1571	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	7	54	dep	eukaryotic	1593:1602	arg1	LLOs					1637:1640	LLOs	1637:1640	LLOs	1637:1640	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	3	55	theme	related	537:543	arg1	enzymes					545:551	their related enzymes	531:551	their related enzymes	531:551	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	4	56	theme	molecular	869:877	arg1	simulations					888:898	molecular dynamics simulations	869:898	molecular dynamics simulations	869:898	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	8	57	theme	future	1746:1751	arg1	studies					1753:1759	future studies	1746:1759	future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein	1746:1841	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	7	58	theme	bacterial	1609:1617	arg1	dynamics					1577:1584	dynamics	1577:1584	dynamics	1577:1584	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	7	58	theme	bacterial	1609:1617	arg1	structure					1563:1571	structure	1563:1571	structure	1563:1571	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	4	59	theme	such	829:832	arg1	techniques					817:826	molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics	798:916	techniques	817:826	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	3	60	theme	biotechnological	564:579	arg1	applications					581:592	diverse biotechnological applications	556:592	diverse biotechnological applications	556:592	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	1	61	theme	important	113:121	arg1	role					123:126	an important role	110:126	an important role	110:126	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	4	62	theme	quantum	837:843	arg1	calculations					855:866	quantum mechanics calculations	837:866	quantum mechanics calculations	837:866	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	6	63	theme	chains	1399:1404	arg1	dynamics					1376:1383	The dynamics	1372:1383	The dynamics of isoprenoid chains embedded within the bilayer	1372:1432	The dynamics of isoprenoid chains embedded within the bilayer are described, and membrane dynamics and related properties are also investigated.					
31769974	8	64	theme	transferring	1768:1779	arg1	mechanism					1781:1789	the transferring mechanism	1764:1789	the transferring mechanism of the oligosaccharide chain to an acceptor protein	1764:1841	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	7	65	with	association	1700:1710	arg1	OSTs					1717:1720	OSTs	1717:1720	OSTs	1717:1720	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	5	66	theme	membrane	1265:1272	arg1	groups					1285:1290	the membrane lipid head groups	1261:1290	the membrane lipid head groups	1261:1290	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	5	67	theme	molecular	1132:1140	arg1	simulations					1151:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations	1120:1161	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	1	68	theme	N-glycosylation	135:149	arg1	pathway					151:157	the N-glycosylation pathway	131:157	the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide	131:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	2	69	from	undecaprenol	398:409	arg1	prokarya					414:421	prokarya	414:421	prokarya	414:421	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	5	70	theme	head	1280:1283	arg1	groups					1285:1290	the membrane lipid head groups	1261:1290	the membrane lipid head groups	1261:1290	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	8	71	theme	oligosaccharide	1798:1812	arg1	chain					1814:1818	the oligosaccharide chain	1794:1818	the oligosaccharide chain	1794:1818	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	3	72	theme	such	770:773	arg1	systems					775:781	such systems	770:781	such systems	770:781	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	2	73	theme	units	455:459	arg1	number					436:441	the number	432:441	the number of isoprene units	432:459	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	5	74	theme	phosphate	1343:1351	arg1	groups					1358:1363	lipid phosphate head groups	1337:1363	the lipid phosphate head groups level	1333:1369	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	0	75	theme	Lazy	4:7	arg1	Life					9:12	The Lazy Life	0:12	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains	0:65	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains.					
31769974	8	76	theme	acceptor	1826:1833	arg1	protein					1835:1841	an acceptor protein	1823:1841	an acceptor protein	1823:1841	These data may support future studies on the transferring mechanism of the oligosaccharide chain to an acceptor protein.					
31769974	3	77	from	processing	716:725	arg1	systems					775:781	such systems	770:781	such systems	770:781	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	7	78	from	structure	1563:1571	arg1	bilayers					1645:1652	bilayers	1645:1652	bilayers	1645:1652	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	3	79	theme	reliable	605:612	arg1	models					618:623	reliable LLO models	605:623	reliable LLO models	605:623	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	0	80	theme	Oligosaccharides	30:45	arg1	Life					9:12	The Lazy Life	0:12	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains	0:65	The Lazy Life of Lipid-Linked Oligosaccharides in All Life Domains.					
31769974	5	81	theme	glycan	1215:1220	arg1	chains					1222:1227	glycan chains	1215:1227	glycan chains	1215:1227	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	7	82	from	dynamics	1577:1584	arg1	bilayers					1645:1652	bilayers	1645:1652	bilayers	1645:1652	Overall, there are similarities regarding the structure and dynamics of the eukaryotic, the bacterial, and the archaeal LLOs in bilayers, which can support the comprehension of their association with OSTs.					
31769974	4	83	theme	eukaryotic	941:950	arg1	LLOs					1093:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs	941:1096	In this work, molecular modeling techniques, such as quantum mechanics calculations, molecular dynamics simulations, and metadynamics were employed to study eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol), and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLOs in membrane bilayers.					
31769974	5	84	theme	metadynamics	1167:1178	arg1	calculations					1180:1191	metadynamics calculations	1167:1191	metadynamics calculations	1167:1191	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	3	85	theme	distinct	630:637	arg1	life					650:653	life	650:653	life	650:653	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	3	85	theme	distinct	630:637	arg1	domains					639:645	distinct domains	630:645	distinct domains of life	630:653	Given the potential relevance of LLOs and their related enzymes to diverse biotechnological applications, obtaining reliable LLO models from distinct domains of life could support further studies on complex formation and their processing by OSTs, as well as protein engineering on such systems.					
31769974	1	86	theme	nascent	291:297	arg1	polypeptide					299:309	a nascent polypeptide	289:309	a nascent polypeptide	289:309	Lipid-linked oligosaccharides (LLOs) play an important role in the N-glycosylation pathway as the donor substrate of oligosaccharyltransferases (OSTs), which are responsible for the en bloc transfer of glycan chains onto a nascent polypeptide.					
31769974	5	87	theme	groups	1358:1363	arg1	level					1365:1369	the lipid phosphate head groups level	1333:1369	the lipid phosphate head groups level	1333:1369	Microsecond molecular dynamics simulations and metadynamics calculations of LLOs revealed that glycan chains are more prone to interact with the membrane lipid head groups, while the PP linkages are positioned at the lipid phosphate head groups level.					
31769974	2	88	theme	LLO	335:337	arg1	component					322:330	The lipid component	312:330	The lipid component of LLO in both eukarya and archaea	312:365	The lipid component of LLO in both eukarya and archaea consists of a dolichol, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species.					
31769974	6	89	theme	related	1475:1481	arg1	properties					1483:1492	related properties	1475:1492	related properties	1475:1492	The dynamics of isoprenoid chains embedded within the bilayer are described, and membrane dynamics and related properties are also investigated.					
30649348	6	0	theme	better	1012:1017	arg1	understanding					1019:1031	a better understanding	1010:1031	a better understanding of how alterations in O-glycosylation influence tumour immune evasion	1010:1101	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	1	1	gly	glycosylation	75:87	arg1	cells					99:103	tumour cells	92:103	tumour cells	92:103	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	1	1	gly	glycosylation	75:87	arg1	modulation					142:151	tumour immune modulation	128:151	tumour immune modulation	128:151	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	6	2	from	alterations	1040:1050	arg1	O-glycosylation					1055:1069	O-glycosylation	1055:1069	O-glycosylation	1055:1069	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	3	3	from	loss	476:479	arg1	impairment					562:571	subsequent impairment	551:571	subsequent impairment of galectin-glycan lattice formation	551:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	3	3	from	loss	476:479	arg1	O-glycans					537:545	human leukocyte antigen Class I O-glycans	505:545	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	5	4	theme	growth	909:914	arg1	factors					916:922	growth factors	909:922	growth factors	909:922	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	0	5	from	Implications	0:11	arg1	evasion					57:63	tumour immune evasion	43:63	tumour immune evasion	43:63	Implications of altered O-glycosylation in tumour immune evasion.					
30649348	2	6	theme	immunity	267:274	arg1	evasion					252:258	evasion	252:258	evasion of CTL immunity by altered O-glycosylation of HLA class I	252:316	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	3	7	theme	leukocyte	511:519	arg1	Class					529:533	human leukocyte antigen Class I	505:535	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	5	8	theme	sialic	950:955	arg1	lectins					990:996	sialic acid-binding immunoglobulin-like lectins	950:996	sialic acid-binding immunoglobulin-like lectins	950:996	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	2	9	theme	recent	156:161	arg1	article					163:169	A recent article	154:169	A recent article	154:169	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	1	10	theme	Aberrant	66:73	arg1	glycosylation					75:87	Aberrant glycosylation	66:87	Aberrant glycosylation on tumour cells	66:103	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	2	11	theme	CTL	263:265	arg1	immunity					267:274	CTL immunity	263:274	CTL immunity	263:274	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	1	12	theme	immune	135:140	arg1	modulation					142:151	tumour immune modulation	128:151	tumour immune modulation	128:151	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	6	13	theme	cancer	1183:1188	arg1	treatment					1190:1198	cancer treatment	1183:1198	cancer treatment	1183:1198	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	5	14	with	interactions	932:943	arg1	lectins					990:996	sialic acid-binding immunoglobulin-like lectins	950:996	sialic acid-binding immunoglobulin-like lectins	950:996	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	5	15	theme	immune	832:837	arg1	escape					839:844	tumour immune escape	825:844	tumour immune escape	825:844	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	16	theme	structures	491:500	arg1	loss					476:479	the loss	472:479	the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation	472:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	0	17	theme	immune	50:55	arg1	evasion					57:63	tumour immune evasion	43:63	tumour immune evasion	43:63	Implications of altered O-glycosylation in tumour immune evasion.					
30649348	0	18	theme	tumour	43:48	arg1	evasion					57:63	tumour immune evasion	43:63	tumour immune evasion	43:63	Implications of altered O-glycosylation in tumour immune evasion.					
30649348	6	19	theme	effective	1149:1157	arg1	options					1171:1177	novel and more effective therapeutic options	1134:1177	novel and more effective therapeutic options for cancer treatment	1134:1198	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	6	20	theme	novel	1134:1138	arg1	options					1171:1177	novel and more effective therapeutic options	1134:1177	novel and more effective therapeutic options for cancer treatment	1134:1198	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	3	21	theme	lymphocyte-mediated	401:419	arg1	immunity					432:439	cytotoxic T lymphocyte-mediated antitumour immunity	389:439	cytotoxic T lymphocyte-mediated antitumour immunity	389:439	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	1	22	theme	tumour	92:97	arg1	cells					99:103	tumour cells	92:103	tumour cells	92:103	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	4	23	theme	killer	664:669	arg1	immunity					692:699	natural killer cell-mediated tumour immunity	656:699	natural killer cell-mediated tumour immunity	656:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	5	24	theme	central	809:815	arg1	role					817:820	a central role	807:820	a central role	807:820	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	4	25	theme	evasion	730:736	arg1	system					738:743	an immune evasion system	720:743	an immune evasion system	720:743	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	4	26	theme	natural	656:662	arg1	killer					664:669	natural killer	656:669	natural killer cell-mediated tumour immunity	656:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	0	27	theme	O-glycosylation	24:38	arg1	Implications					0:11	Implications	0:11	Implications of altered O-glycosylation in tumour immune evasion	0:63	Implications of altered O-glycosylation in tumour immune evasion.					
30649348	3	28	theme	subsequent	551:560	arg1	impairment					562:571	subsequent impairment	551:571	subsequent impairment of galectin-glycan lattice formation	551:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	2	29	theme	altered	279:285	arg1	O-glycosylation					287:301	altered O-glycosylation	279:301	altered O-glycosylation of HLA class I	279:316	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	4	30	from	action	633:638	arg1	immunity					692:699	natural killer cell-mediated tumour immunity	656:699	natural killer cell-mediated tumour immunity	656:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	4	31	theme	immunosuppressive	615:631	arg1	action					633:638	The immunosuppressive action	611:638	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity	611:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	0	32	theme	altered	16:22	arg1	O-glycosylation					24:38	altered O-glycosylation	16:38	altered O-glycosylation	16:38	Implications of altered O-glycosylation in tumour immune evasion.					
30649348	5	33	theme	acid-binding	957:968	arg1	lectins					990:996	sialic acid-binding immunoglobulin-like lectins	950:996	sialic acid-binding immunoglobulin-like lectins	950:996	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	5	34	theme	immunoglobulin-like	970:988	arg1	lectins					990:996	sialic acid-binding immunoglobulin-like lectins	950:996	sialic acid-binding immunoglobulin-like lectins	950:996	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	35	theme	Class	529:533	arg1	O-glycans					537:545	human leukocyte antigen Class I O-glycans	505:545	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	4	36	theme	tumour	685:690	arg1	immunity					692:699	natural killer cell-mediated tumour immunity	656:699	natural killer cell-mediated tumour immunity	656:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	3	37	theme	cancer	369:374	arg1	cells					376:380	bladder cancer cells	361:380	bladder cancer cells	361:380	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	6	38	theme	immune	1088:1093	arg1	evasion					1095:1101	tumour immune evasion	1081:1101	tumour immune evasion	1081:1101	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	3	39	theme	antitumour	421:430	arg1	immunity					432:439	cytotoxic T lymphocyte-mediated antitumour immunity	389:439	cytotoxic T lymphocyte-mediated antitumour immunity	389:439	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	6	40	theme	tumour	1081:1086	arg1	evasion					1095:1101	tumour immune evasion	1081:1101	tumour immune evasion	1081:1101	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	5	41	theme	immunoregulatory	878:893	arg1	cytokines					895:903	immunoregulatory cytokines	878:903	immunoregulatory cytokines	878:903	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	6	42	theme	options	1171:1177	arg1	development					1119:1129	the development	1115:1129	the development of novel and more effective therapeutic options for cancer treatment	1115:1198	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	5	43	theme	factors	916:922	arg1	production					864:873	the production	860:873	the production of immunoregulatory cytokines and growth factors	860:922	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	44	theme	bladder	361:367	arg1	cells					376:380	bladder cancer cells	361:380	bladder cancer cells	361:380	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	3	45	theme	novel	446:450	arg1	mechanism					452:460	a novel mechanism	444:460	a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation	444:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	2	46	theme	Sutoh	213:217	arg1	Yoneyama					219:226	Biochemistry (Sutoh Yoneyama	199:226	Biochemistry (Sutoh Yoneyama	199:226	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	4	47	theme	immune	723:728	arg1	system					738:743	an immune evasion system	720:743	an immune evasion system	720:743	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	2	48	theme	Biochemistry	199:210	arg1	Yoneyama					219:226	Biochemistry (Sutoh Yoneyama	199:226	Biochemistry (Sutoh Yoneyama	199:226	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	3	49	theme	formation	600:608	arg1	impairment					562:571	subsequent impairment	551:571	subsequent impairment of galectin-glycan lattice formation	551:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	3	49	theme	formation	600:608	arg1	O-glycans					537:545	human leukocyte antigen Class I O-glycans	505:545	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	1	50	theme	tumour	128:133	arg1	modulation					142:151	tumour immune modulation	128:151	tumour immune modulation	128:151	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
30649348	3	51	theme	antigen	521:527	arg1	Class					529:533	human leukocyte antigen Class I	505:535	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	6	52	theme	therapeutic	1159:1169	arg1	options					1171:1177	novel and more effective therapeutic options	1134:1177	novel and more effective therapeutic options for cancer treatment	1134:1198	Therefore, a better understanding of how alterations in O-glycosylation influence tumour immune evasion will enable the development of novel and more effective therapeutic options for cancer treatment.					
30649348	5	53	gly	sialylated	759:768	arg1	O-glycans					770:778	sialylated O-glycans	759:778	sialylated O-glycans	759:778	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	54	theme	human	505:509	arg1	Class					529:533	human leukocyte antigen Class I	505:535	human leukocyte antigen Class I O-glycans	505:545	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	2	55	theme	HLA	306:308	arg1	class					310:314	HLA class I	306:316	HLA class I	306:316	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	2	56	theme	class	310:314	arg1	O-glycosylation					287:301	altered O-glycosylation	279:301	altered O-glycosylation of HLA class I	279:316	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	2	57	gly	O-glycosylation	287:301	arg1	class					310:314	HLA class I	306:316	HLA class I	306:316	A recent article published in The Journal of Biochemistry (Sutoh Yoneyama et al., A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I, J. Biochem.					
30649348	5	58	theme	cytokines	895:903	arg1	production					864:873	the production	860:873	the production of immunoregulatory cytokines and growth factors	860:922	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	59	theme	galectin-glycan	576:590	arg1	formation					600:608	galectin-glycan lattice formation	576:608	galectin-glycan lattice formation	576:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	5	60	theme	tumour	825:830	arg1	escape					839:844	tumour immune escape	825:844	tumour immune escape	825:844	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	61	theme	Core	484:487	arg1	structures					491:500	Core 2 structures	484:500	Core 2 structures	484:500	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	4	62	theme	O-glycans	643:651	arg1	action					633:638	The immunosuppressive action	611:638	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity	611:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	5	63	theme	sialylated	759:768	arg1	O-glycans					770:778	sialylated O-glycans	759:778	sialylated O-glycans	759:778	Furthermore, sialylated O-glycans have been proposed to play a central role in tumour immune escape by modulating the production of immunoregulatory cytokines and growth factors through interactions with sialic acid-binding immunoglobulin-like lectins.					
30649348	3	64	theme	lattice	592:598	arg1	formation					600:608	galectin-glycan lattice formation	576:608	galectin-glycan lattice formation	576:608	2017;161:479-492) showed that bladder cancer cells evaded cytotoxic T lymphocyte-mediated antitumour immunity by a novel mechanism involving the loss of Core 2 structures on human leukocyte antigen Class I O-glycans and subsequent impairment of galectin-glycan lattice formation.					
30649348	4	65	theme	cell-mediated	671:683	arg1	immunity					692:699	natural killer cell-mediated tumour immunity	656:699	natural killer cell-mediated tumour immunity	656:699	The immunosuppressive action of O-glycans on natural killer cell-mediated tumour immunity is also considered an immune evasion system.					
30649348	1	66	from	glycosylation	75:87	arg1	cells					99:103	tumour cells	92:103	tumour cells	92:103	Aberrant glycosylation on tumour cells has been implicated in tumour immune modulation.					
31409668	9	0	from	proportion	1400:1409	arg1	SIgA					1463:1466	salivary SIgA	1454:1466	salivary SIgA	1454:1466	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	10	1	theme	maternal	1823:1830	arg1	protection					1845:1854	maternal and new-born protection	1823:1854	maternal and new-born protection	1823:1854	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	7	2	theme	fucosylated	1088:1098	arg1	species					1136:1142	fucosylated and both fucosylated and sialylated species	1088:1142	fucosylated and both fucosylated and sialylated species	1088:1142	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	2	3	attach	present	355:361	arg1	milk					373:376	breast milk	366:376	breast milk	366:376	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	2	3	attach	present	355:361	arg2	SIgA					294:297	SIgA	294:297	SIgA	294:297	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	9	4	theme	hormonal	1600:1607	arg1	changes					1609:1615	the hormonal changes	1596:1615	the hormonal changes during vaginal delivery	1596:1639	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	4	5	gly	N-glycosylation	636:650	arg1	SIgA					655:658	SIgA	655:658	SIgA purified from mature breast milk and saliva	655:702	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	10	6	theme	structural	1702:1711	arg1	details					1713:1719	the structural details	1698:1719	the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development	1698:1877	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	5	7	theme	glycan	833:838	arg1	compositions					840:851	89 glycan compositions	830:851	89 glycan compositions that may correspond to over 250 structures	830:894	Our results revealed a greater diversity than previously reported, with 89 glycan compositions that may correspond to over 250 structures.					
31409668	6	8	theme	glycan	921:926	arg1	compositions					928:939	54 glycan compositions	918:939	54 glycan compositions	918:939	Among these glycans, 54 glycan compositions were characterized as body-fluid specific.					
31409668	7	9	gly	fucosylated	1088:1098	arg1	species					1136:1142	fucosylated and both fucosylated and sialylated species	1088:1142	fucosylated and both fucosylated and sialylated species	1088:1142	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	1	10	theme	immunological	198:210	arg1	mechanisms					212:221	the main immunological mechanisms	189:221	the main immunological mechanisms of defense against the potential attack of pathogens	189:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	4	11	theme	glycomic	610:617	arg1	study					619:623	a glycomic study	608:623	a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants	608:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	9	12	theme	glycans	1443:1449	arg1	proportion					1400:1409	an increased proportion	1387:1409	an increased proportion of high mannose and fucosylated glycans in salivary SIgA	1387:1466	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	11	13	theme	detail	2028:2033	arg1	dimension					2004:2012	this dimension	1999:2012	this dimension of structural detail	1999:2033	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	4	14	theme	breast	681:686	arg1	milk					688:691	mature breast milk	674:691	mature breast milk	674:691	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	3	15	theme	health	561:566	arg1	maintenance					568:578	health maintenance	561:578	health maintenance	561:578	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	9	16	theme	salivary	1454:1461	arg1	SIgA					1463:1466	salivary SIgA	1454:1466	salivary SIgA	1454:1466	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	7	17	theme	unique	998:1003	arg1	compositions					1014:1025	these unique N-Glycan compositions	992:1025	these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma	992:1081	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	10	18	theme	S	1767:1767	arg1	N-Glycans					1773:1781	(S)IgA N-Glycans	1766:1781	(S)IgA N-Glycans	1766:1781	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	9	19	gly	fucosylated	1431:1441	arg1	glycans					1443:1449	fucosylated glycans	1431:1449	fucosylated glycans	1431:1449	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	4	20	theme	SIgA	655:658	arg1	IgA					716:718	plasma IgA	709:718	plasma IgA from the same lactating participants	709:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	4	20	theme	SIgA	655:658	arg1	N-glycosylation					636:650	N-glycosylation	636:650	N-glycosylation of SIgA purified from mature breast milk and saliva	636:702	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	11	21	theme	recombinant	1902:1912	arg1	molecules					1918:1926	optimal recombinant IgA molecules	1894:1926	optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces	1894:1976	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	4	22	from	participants	744:755	arg1	IgA					716:718	plasma IgA	709:718	plasma IgA from the same lactating participants	709:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	4	22	from	participants	744:755	arg1	N-glycosylation					636:650	N-glycosylation	636:650	N-glycosylation of SIgA purified from mature breast milk and saliva	636:702	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	1	23	theme	mucosal	153:159	arg1	immunity					161:168	mucosal immunity	153:168	mucosal immunity	153:168	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	2	24	located	present	355:361	arg1	milk					373:376	breast milk	366:376	breast milk	366:376	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	2	24	located	present	355:361	arg2	SIgA					294:297	SIgA	294:297	SIgA	294:297	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	3	25	gly	N-glycosylated	499:512	arg1	components					469:478	the different components	455:478	the different components of SIgA	455:486	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	3	25	gly	N-glycosylated	499:512	arg1	N-glycosylated					499:512	N-glycosylated	499:512	N-glycosylated	499:512	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	4	26	theme	same	729:732	arg1	participants					744:755	the same lactating participants	725:755	the same lactating participants	725:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	7	27	theme	salivary	1156:1163	arg1	SIgA					1165:1168	salivary SIgA	1156:1168	salivary SIgA	1156:1168	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	0	28	theme	Body	52:55	arg1	Variations					72:81	Important Body Fluid-specific Variations	42:81	Important Body Fluid-specific Variations in Lactating Mothers	42:102	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	10	29	from	roles	1757:1761	arg1	protection					1845:1854	maternal and new-born protection	1823:1854	maternal and new-born protection	1823:1854	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	10	29	from	roles	1757:1761	arg1	tissues					1796:1802	different tissues	1786:1802	different tissues	1786:1802	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	10	29	from	roles	1757:1761	arg1	development					1867:1877	infant development	1860:1877	infant development	1860:1877	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	0	30	theme	Immunoglobulin	0:13	arg1	N-glycosylation					17:31	Immunoglobulin A N-glycosylation	0:31	Immunoglobulin A N-glycosylation	0:31	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	1	31	theme	Immunoglobulin	115:128	arg1	SIgA					133:136	SIgA	133:136	SIgA	133:136	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	1	31	theme	Immunoglobulin	115:128	arg1	A					130:130	Secretory Immunoglobulin A	105:130	Secretory Immunoglobulin A (SIgA)	105:137	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	3	32	contain	have	535:538	arg2	role					553:556	an essential role	540:556	an essential role	540:556	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	3	32	contain	have	535:538	arg1	N-Glycans					525:533	these N-Glycans	519:533	these N-Glycans	519:533	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	4	33	theme	plasma	709:714	arg1	IgA					716:718	plasma IgA	709:718	plasma IgA from the same lactating participants	709:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	5	34	theme	greater	781:787	arg1	diversity					789:797	a greater diversity	779:797	a greater diversity than previously reported	779:822	Our results revealed a greater diversity than previously reported, with 89 glycan compositions that may correspond to over 250 structures.					
31409668	9	35	theme	fucosylated	1530:1540	arg1	glycans					1557:1563	both fucosylated and sialylated glycans	1525:1563	glycans	1557:1563	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	8	36	theme	mode	1284:1287	arg1	effect					1265:1270	the effect	1261:1270	the effect of delivery mode on (S)IgA glycosylation	1261:1311	In addition, we evaluated the effect of delivery mode on (S)IgA glycosylation.					
31409668	0	37	theme	Important	42:50	arg1	Variations					72:81	Important Body Fluid-specific Variations	42:81	Important Body Fluid-specific Variations in Lactating Mothers	42:102	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	9	38	theme	sialylated	1546:1555	arg1	glycans					1557:1563	both fucosylated and sialylated glycans	1525:1563	glycans	1557:1563	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	7	39	from	plasma	1076:1081	arg1	IgA					1067:1069	IgA	1067:1069	IgA	1067:1069	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	7	39	from	plasma	1076:1081	arg1	milk					1058:1061	mature milk	1051:1061	mature milk	1051:1061	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	10	40	theme	IgA	1769:1771	arg1	N-Glycans					1773:1781	(S)IgA N-Glycans	1766:1781	(S)IgA N-Glycans	1766:1781	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	0	41	theme	Fluid-specific	57:70	arg1	Variations					72:81	Important Body Fluid-specific Variations	42:81	Important Body Fluid-specific Variations in Lactating Mothers	42:102	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	2	42	theme	mammary	334:340	arg1	gland					342:346	the mammary gland	330:346	the mammary gland	330:346	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	1	43	theme	defense	226:232	arg1	mechanisms					212:221	the main immunological mechanisms	189:221	the main immunological mechanisms of defense against the potential attack of pathogens	189:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	5	44	dep	diversity	789:797	arg1	reported					815:822	reported	815:822	reported	815:822	Our results revealed a greater diversity than previously reported, with 89 glycan compositions that may correspond to over 250 structures.					
31409668	2	45	theme	breast	366:371	arg1	milk					373:376	breast milk	366:376	breast milk	366:376	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	3	46	theme	SIgA	483:486	arg1	components					469:478	the different components	455:478	the different components of SIgA	455:486	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	3	46	theme	SIgA	483:486	arg1	N-glycosylated					499:512	N-glycosylated	499:512	N-glycosylated	499:512	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	9	47	theme	vaginal	1360:1366	arg1	delivery					1368:1375	vaginal delivery	1360:1375	vaginal delivery	1360:1375	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	7	48	theme	complex	1216:1222	arg1	N-Glycans					1224:1232	complex N-Glycans	1216:1232	complex N-Glycans	1216:1232	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	1	49	theme	pathogens	266:274	arg1	attack					256:261	the potential attack	242:261	the potential attack of pathogens	242:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	10	50	theme	new	1736:1738	arg1	dimension					1740:1748	a new dimension	1734:1748	a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development	1734:1877	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	10	51	theme	infant	1860:1865	arg1	development					1867:1877	infant development	1860:1877	infant development	1860:1877	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	9	52	theme	increased	1390:1398	arg1	proportion					1400:1409	an increased proportion	1387:1409	an increased proportion of high mannose and fucosylated glycans in salivary SIgA	1387:1466	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	9	53	theme	vaginal	1624:1630	arg1	delivery					1632:1639	vaginal delivery	1624:1639	vaginal delivery	1624:1639	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	2	54	from	present	355:361	arg1	milk					373:376	breast milk	366:376	breast milk	366:376	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	7	55	theme	mature	1051:1056	arg1	milk					1058:1061	mature milk	1051:1061	mature milk	1051:1061	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	3	56	theme	essential	543:551	arg1	role					553:556	an essential role	540:556	an essential role	540:556	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	11	57	theme	mucosal	1961:1967	arg1	surfaces					1969:1976	mucosal surfaces	1961:1976	mucosal surfaces	1961:1976	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	9	58	theme	mannose	1419:1425	arg1	proportion					1400:1409	an increased proportion	1387:1409	an increased proportion of high mannose and fucosylated glycans in salivary SIgA	1387:1466	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	9	59	theme	plasma	1654:1659	arg1	IgA					1672:1674	plasma and saliva IgA	1654:1674	IgA	1672:1674	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	9	60	theme	high	1414:1417	arg1	mannose					1419:1425	high mannose	1414:1425	high mannose	1414:1425	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	1	61	theme	mechanisms	212:221	arg1	mechanisms					212:221	the main immunological mechanisms	189:221	the main immunological mechanisms of defense against the potential attack of pathogens	189:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	1	61	theme	mechanisms	212:221	arg1	one					182:184	one	182:184	one	182:184	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	9	62	theme	saliva	1665:1670	arg1	IgA					1672:1674	plasma and saliva IgA	1654:1674	IgA	1672:1674	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	7	63	theme	N-Glycan	1005:1012	arg1	compositions					1014:1025	these unique N-Glycan compositions	992:1025	these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma	992:1081	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	4	64	theme	mature	674:679	arg1	milk					688:691	mature breast milk	674:691	mature breast milk	674:691	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	11	65	theme	IgA	1914:1916	arg1	molecules					1918:1926	optimal recombinant IgA molecules	1894:1926	optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces	1894:1976	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	11	66	theme	structural	2017:2026	arg1	detail					2028:2033	structural detail	2017:2033	structural detail	2017:2033	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	0	67	theme	Lactating	86:94	arg1	Mothers					96:102	Lactating Mothers	86:102	Lactating Mothers	86:102	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	11	68	theme	optimal	1894:1900	arg1	molecules					1918:1926	optimal recombinant IgA molecules	1894:1926	optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces	1894:1976	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	1	69	theme	main	193:196	arg1	mechanisms					212:221	the main immunological mechanisms	189:221	the main immunological mechanisms of defense against the potential attack of pathogens	189:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	8	70	theme	delivery	1275:1282	arg1	mode					1284:1287	delivery mode	1275:1287	delivery mode	1275:1287	In addition, we evaluated the effect of delivery mode on (S)IgA glycosylation.					
31409668	7	71	theme	unique	1174:1179	arg1	structures					1181:1190	the unique structures	1170:1190	the unique structures	1170:1190	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	9	72	theme	high	1482:1485	arg1	mannose					1487:1493	high mannose	1482:1493	high mannose	1482:1493	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	7	73	dep	species	1136:1142	arg1	whereas					1145:1151	whereas	1145:1151	whereas	1145:1151	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	8	74	from	effect	1265:1270	arg1	glycosylation					1299:1311	(S)IgA glycosylation	1292:1311	(S)IgA glycosylation	1292:1311	In addition, we evaluated the effect of delivery mode on (S)IgA glycosylation.					
31409668	0	75	from	Variations	72:81	arg1	Mothers					96:102	Lactating Mothers	86:102	Lactating Mothers	86:102	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	8	76	theme	S	1293:1293	arg1	glycosylation					1299:1311	(S)IgA glycosylation	1292:1311	(S)IgA glycosylation	1292:1311	In addition, we evaluated the effect of delivery mode on (S)IgA glycosylation.					
31409668	1	77	dep	central	142:148	arg1	represents					171:180	represents	171:180	is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens	139:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	8	78	theme	IgA	1295:1297	arg1	glycosylation					1299:1311	(S)IgA glycosylation	1292:1311	(S)IgA glycosylation	1292:1311	In addition, we evaluated the effect of delivery mode on (S)IgA glycosylation.					
31409668	0	79	theme	A	15:15	arg1	N-glycosylation					17:31	Immunoglobulin A N-glycosylation	0:31	Immunoglobulin A N-glycosylation	0:31	Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.					
31409668	1	80	theme	Secretory	105:113	arg1	SIgA					133:136	SIgA	133:136	SIgA	133:136	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	1	80	theme	Secretory	105:113	arg1	A					130:130	Secretory Immunoglobulin A	105:130	Secretory Immunoglobulin A (SIgA)	105:137	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31409668	2	81	theme	passive	407:413	arg1	immunity					415:422	the passive immunity	403:422	the passive immunity of the newborn	403:437	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	10	82	theme	different	1786:1794	arg1	tissues					1796:1802	different tissues	1786:1802	different tissues	1786:1802	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	7	83	theme	fucosylated	1109:1119	arg1	species					1136:1142	fucosylated and both fucosylated and sialylated species	1088:1142	fucosylated and both fucosylated and sialylated species	1088:1142	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	11	84	theme	molecules	1918:1926	arg1	design					1884:1889	The design	1880:1889	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces	1880:1976	The design of optimal recombinant IgA molecules specifically targeted to protect mucosal surfaces will need to include this dimension of structural detail.					
31409668	9	85	theme	fucosylated	1431:1441	arg1	glycans					1443:1449	fucosylated glycans	1431:1449	fucosylated glycans	1431:1449	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	10	86	theme	N-Glycans	1773:1781	arg1	roles					1757:1761	the roles	1753:1761	the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development	1753:1877	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	7	87	gly	fucosylated	1109:1119	arg1	species					1136:1142	fucosylated and both fucosylated and sialylated species	1088:1142	fucosylated and both fucosylated and sialylated species	1088:1142	Most of these unique N-Glycan compositions identified in SIgA from mature milk and IgA from plasma were fucosylated and both fucosylated and sialylated species, whereas in salivary SIgA the unique structures were mainly undecorated complex N-Glycans.					
31409668	2	88	theme	vital	389:393	arg1	role					395:398	a vital role	387:398	a vital role	387:398	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	9	89	theme	Lactating	1314:1322	arg1	participants					1324:1335	Lactating participants	1314:1335	Lactating participants who had given birth by vaginal delivery	1314:1375	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	2	90	from	milk	373:376	arg1	present					355:361	present	355:361	present	355:361	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	2	91	theme	newborn	431:437	arg1	immunity					415:422	the passive immunity	403:422	the passive immunity of the newborn	403:437	During lactation SIgA is produced by plasmablasts in the mammary gland and is present in breast milk, playing a vital role in the passive immunity of the newborn.					
31409668	9	92	gly	fucosylated	1530:1540	arg1	glycans					1557:1563	both fucosylated and sialylated glycans	1525:1563	glycans	1557:1563	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	9	93	theme	plasma	1568:1573	arg1	IgA					1575:1577	plasma IgA	1568:1577	plasma IgA	1568:1577	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	9	94	gly	sialylated	1546:1555	arg1	glycans					1557:1563	both fucosylated and sialylated glycans	1525:1563	glycans	1557:1563	Lactating participants who had given birth by vaginal delivery presented an increased proportion of high mannose and fucosylated glycans in salivary SIgA, and selected high mannose, fucosylated, sialylated, and both fucosylated and sialylated glycans in plasma IgA, indicating that the hormonal changes during vaginal delivery could affect plasma and saliva IgA.					
31409668	4	95	theme	lactating	734:742	arg1	participants					744:755	the same lactating participants	725:755	the same lactating participants	725:755	In this work, we performed a glycomic study to compare N-glycosylation of SIgA purified from mature breast milk and saliva, and plasma IgA from the same lactating participants.					
31409668	3	96	theme	different	459:467	arg1	components					469:478	the different components	455:478	the different components of SIgA	455:486	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	3	96	theme	different	459:467	arg1	N-glycosylated					499:512	N-glycosylated	499:512	N-glycosylated	499:512	Interestingly, the different components of SIgA are highly N-glycosylated, and these N-Glycans have an essential role in health maintenance.					
31409668	10	97	theme	new-born	1836:1843	arg1	protection					1845:1854	maternal and new-born protection	1823:1854	maternal and new-born protection	1823:1854	These results reveal the structural details that provide a new dimension to the roles of (S)IgA N-Glycans in different tissues, and especially in maternal and new-born protection and infant development.					
31409668	1	98	theme	potential	246:254	arg1	attack					256:261	the potential attack	242:261	the potential attack of pathogens	242:274	Secretory Immunoglobulin A (SIgA) is central to mucosal immunity: represents one of the main immunological mechanisms of defense against the potential attack of pathogens.					
31350337	5	0	theme	similar	1178:1184	arg1	glycans					1186:1192	similar glycans	1178:1192	similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model	1178:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	5	1	theme	infection	1437:1445	arg1	model					1447:1451	a Galleria mellonella larva infection model	1409:1451	a Galleria mellonella larva infection model	1409:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	2	theme	gene-knockout	673:685	arg1	mutants					687:693	gene-knockout mutants	673:693	gene-knockout mutants	673:693	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	0	3	theme	bacterial	87:95	arg1	fitness					97:103	bacterial fitness	87:103	bacterial fitness	87:103	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	1	4	theme	many	185:188	arg1	bacteria					204:211	many Gram-negative bacteria	185:211	many Gram-negative bacteria living in the rhizosphere	185:237	The Burkholderia genus encompasses many Gram-negative bacteria living in the rhizosphere.					
31350337	3	5	theme	protein	442:448	arg1	glycosylation					450:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	4	6	theme	structure	968:976	arg1	translocation					911:923	membrane translocation	902:923	membrane translocation	902:923	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	6	theme	structure	968:976	arg1	assembly					888:895	assembly	888:895	assembly	888:895	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	6	theme	structure	968:976	arg1	synthesis					877:885	synthesis	877:885	synthesis	877:885	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	7	theme	necessary	863:871	arg1	cluster					855:861	a B. cenocepacia O-glycosylation (ogc) gene cluster	811:861	a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	811:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	5	8	dep	Galleria	1411:1418	arg1	mellonella					1420:1429	mellonella	1420:1429	mellonella	1420:1429	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	9	theme	ogc	845:847	arg1	cluster					855:861	a B. cenocepacia O-glycosylation (ogc) gene cluster	811:861	a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	811:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	5	10	dep	species	1214:1220	arg1	thailandensis					1226:1238	B. thailandensis	1223:1238	B. thailandensis	1223:1238	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	5	10	dep	species	1214:1220	arg1	gladioli					1244:1251	B. gladioli	1241:1251	B. gladioli	1241:1251	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	5	10	dep	species	1214:1220	arg1	pseudomallei					1261:1272	B. pseudomallei	1258:1272	B. pseudomallei	1258:1272	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	3	11	link	O-linked	433:440	arg1	glycosylation					450:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	5	12	theme	bacterial	1386:1394	arg1	clearance					1396:1404	bacterial clearance	1386:1404	bacterial clearance in a Galleria mellonella larva infection model	1386:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	3	13	theme	cenocepacia	421:431	arg1	glycosylation					450:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	4	14	theme	O-glycan	943:950	arg1	translocation					911:923	membrane translocation	902:923	membrane translocation	902:923	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	14	theme	O-glycan	943:950	arg1	assembly					888:895	assembly	888:895	assembly	888:895	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	14	theme	O-glycan	943:950	arg1	synthesis					877:885	synthesis	877:885	synthesis	877:885	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	3	15	theme	therapeutic	613:623	arg1	targets					625:631	potential therapeutic targets	603:631	potential therapeutic targets	603:631	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	1	16	theme	Gram-negative	190:202	arg1	bacteria					204:211	many Gram-negative bacteria	185:211	many Gram-negative bacteria living in the rhizosphere	185:237	The Burkholderia genus encompasses many Gram-negative bacteria living in the rhizosphere.					
31350337	0	17	theme	glycan	117:122	arg1	immunogenicity					124:137	glycan immunogenicity	117:137	glycan immunogenicity in humans	117:147	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	4	18	theme	lipid-linked	930:941	arg1	O-glycan					943:950	a lipid-linked O-glycan	928:950	a lipid-linked O-glycan	928:950	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	7	19	theme	general	1688:1694	arg1	O-glycosylation					1704:1718	general protein O-glycosylation	1688:1718	general protein O-glycosylation	1688:1718	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	5	20	from	glycans	1186:1192	arg1	species					1214:1220	the Burkholderia species	1197:1220	the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei	1197:1272	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	21	theme	1,3	1021:1023	arg1	trisaccharide					1035:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	0	22	theme	general	2:8	arg1	machinery					34:42	A general protein O-glycosylation machinery	0:42	A general protein O-glycosylation machinery conserved in Burkholderia species	0:76	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	5	23	theme	glycoproteins	1159:1171	arg1	production					1145:1154	the production	1141:1154	the production of glycoproteins	1141:1171	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	2	24	theme	specific	370:377	arg1	proteins					398:405	specific lipopolysaccharide proteins	370:405	specific lipopolysaccharide proteins	370:405	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	6	25	theme	O-glycan-specific	1606:1622	arg1	antibodies					1624:1633	O-glycan-specific antibodies	1606:1633	O-glycan-specific antibodies	1606:1633	Finally, our experiments revealed that patients infected with B. cenocepacia, Burkholderia multivorans, B. pseudomallei, or Burkholderia mallei develop O-glycan-specific antibodies.					
31350337	4	26	theme	structural	776:785	arg1	analyses					787:794	NMR-based structural analyses	766:794	NMR-based structural analyses	766:794	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	5	27	dep	glycans	1186:1192	arg1	show					1290:1293	show	1290:1293	show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model	1290:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	7	28	theme	O-glycosylation	1704:1718	arg1	importance					1674:1683	the importance	1670:1683	the importance of general protein O-glycosylation in the biology of the Burkholderia genus	1670:1759	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	7	28	theme	O-glycosylation	1704:1718	arg1	potential					1769:1777	its potential	1765:1777	its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections	1765:1867	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	5	29	theme	bacterial	1352:1360	arg1	fitness					1362:1368	bacterial fitness	1352:1368	bacterial fitness	1352:1368	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	30	theme	NMR-based	766:774	arg1	analyses					787:794	NMR-based structural analyses	766:794	NMR-based structural analyses	766:794	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	0	31	theme	O-glycosylation	18:32	arg1	machinery					34:42	A general protein O-glycosylation machinery	0:42	A general protein O-glycosylation machinery conserved in Burkholderia species	0:76	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	7	32	theme	Burkholderia	1742:1753	arg1	genus					1755:1759	the Burkholderia genus	1738:1759	the Burkholderia genus	1738:1759	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	4	33	theme	β-Gal-	999:1004	arg1	trisaccharide					1035:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	7	34	from	potential	1769:1777	arg1	biology					1727:1733	the biology	1723:1733	the biology of the Burkholderia genus	1723:1759	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	4	35	theme	gene	850:853	arg1	cluster					855:861	a B. cenocepacia O-glycosylation (ogc) gene cluster	811:861	a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	811:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	0	36	theme	protein	10:16	arg1	machinery					34:42	A general protein O-glycosylation machinery	0:42	A general protein O-glycosylation machinery conserved in Burkholderia species	0:76	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	7	37	theme	genus	1755:1759	arg1	biology					1727:1733	the biology	1723:1733	the biology of the Burkholderia genus	1723:1759	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	2	38	theme	clinical	337:344	arg1	interventions					346:358	clinical interventions	337:358	clinical interventions targeting specific lipopolysaccharide proteins	337:405	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	5	39	gly	glycoproteins	1159:1171	arg1	glycoproteins					1159:1171	glycoproteins	1159:1171	glycoproteins	1159:1171	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	40	theme	LC-MS-based	727:737	arg1	analyses					739:746	LC-MS-based analyses	727:746	LC-MS-based analyses of O-glycans	727:759	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	3	41	theme	chemical	491:498	arg1	unknown					578:584	unknown	578:584	unknown	578:584	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	3	41	theme	chemical	491:498	arg1	structure					500:508	the chemical structure	487:508	the chemical structure of the O-glycan	487:524	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	4	42	theme	bioinformatics	646:659	arg1	approaches					661:670	bioinformatics approaches	646:670	bioinformatics approaches	646:670	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	43	theme	1,3	1006:1008	arg1	trisaccharide					1035:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	5	44	theme	ogc	1074:1076	arg1	cluster					1078:1084	the ogc cluster	1070:1084	the ogc cluster	1070:1084	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	4	45	theme	O-glycans	751:759	arg1	mutants					687:693	gene-knockout mutants	673:693	gene-knockout mutants	673:693	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	45	theme	O-glycans	751:759	arg1	analyses					787:794	NMR-based structural analyses	766:794	NMR-based structural analyses	766:794	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	45	theme	O-glycans	751:759	arg1	proteins					717:724	purified recombinant proteins	696:724	purified recombinant proteins	696:724	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	45	theme	O-glycans	751:759	arg1	analyses					739:746	LC-MS-based analyses	727:746	LC-MS-based analyses of O-glycans	727:759	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	45	theme	O-glycans	751:759	arg1	approaches					661:670	bioinformatics approaches	646:670	bioinformatics approaches	646:670	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	3	46	theme	O-glycan	517:524	arg1	machinery					534:542	the machinery	530:542	the machinery required for its biosynthesis	530:572	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	3	46	theme	O-glycan	517:524	arg1	unknown					578:584	unknown	578:584	unknown	578:584	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	3	46	theme	O-glycan	517:524	arg1	structure					500:508	the chemical structure	487:508	the chemical structure of the O-glycan	487:524	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	4	47	theme	membrane	902:909	arg1	translocation					911:923	membrane translocation	902:923	membrane translocation	902:923	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	3	48	theme	O-linked	433:440	arg1	glycosylation					450:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	0	49	theme	Burkholderia	57:68	arg1	species					70:76	Burkholderia species	57:76	Burkholderia species	57:76	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	4	50	theme	-α-GalNAc-	1010:1019	arg1	trisaccharide					1035:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	51	theme	B.	813:814	arg1	cluster					855:861	a B. cenocepacia O-glycosylation (ogc) gene cluster	811:861	a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	811:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	52	link	lipid-linked	930:941	arg1	O-glycan					943:950	a lipid-linked O-glycan	928:950	a lipid-linked O-glycan	928:950	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	7	53	theme	protein	1696:1702	arg1	O-glycosylation					1704:1718	general protein O-glycosylation	1688:1718	general protein O-glycosylation	1688:1718	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	7	54	from	importance	1674:1683	arg1	biology					1727:1733	the biology	1723:1733	the biology of the Burkholderia genus	1723:1759	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	0	55	from	immunogenicity	124:137	arg1	humans					142:147	humans	142:147	humans	142:147	A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans.					
31350337	2	56	theme	Burkholderia	245:256	arg1	species					258:264	Some Burkholderia species	240:264	Some Burkholderia species	240:264	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	5	57	theme	protein	1311:1317	arg1	O-glycosylation					1319:1333	protein O-glycosylation	1311:1333	protein O-glycosylation	1311:1333	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	5	58	from	clearance	1396:1404	arg1	model					1447:1451	a Galleria mellonella larva infection model	1409:1451	a Galleria mellonella larva infection model	1409:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	7	59	theme	Burkholderia	1845:1856	arg1	infections					1858:1867	Burkholderia infections	1845:1867	Burkholderia infections	1845:1867	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	4	60	theme	O-glycosylation	828:842	arg1	cluster					855:861	a B. cenocepacia O-glycosylation (ogc) gene cluster	811:861	a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	811:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	61	dep	B.	813:814	arg1	cenocepacia					816:826	cenocepacia	816:826	cenocepacia	816:826	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	62	theme	recombinant	705:715	arg1	proteins					717:724	purified recombinant proteins	696:724	purified recombinant proteins	696:724	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	63	theme	consists	985:992	arg1	translocation					911:923	membrane translocation	902:923	membrane translocation	902:923	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	63	theme	consists	985:992	arg1	assembly					888:895	assembly	888:895	assembly	888:895	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	63	theme	consists	985:992	arg1	synthesis					877:885	synthesis	877:885	synthesis	877:885	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	5	64	theme	Burkholderia	1106:1117	arg1	genus					1119:1123	the Burkholderia genus	1102:1123	the Burkholderia genus	1102:1123	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	5	65	theme	Galleria	1411:1418	arg1	model					1447:1451	a Galleria mellonella larva infection model	1409:1451	a Galleria mellonella larva infection model	1409:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	2	66	theme	lipopolysaccharide	379:396	arg1	proteins					398:405	specific lipopolysaccharide proteins	370:405	specific lipopolysaccharide proteins	370:405	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	4	67	theme	purified	696:703	arg1	proteins					717:724	purified recombinant proteins	696:724	purified recombinant proteins	696:724	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	4	68	theme	-β-GalNAc	1025:1033	arg1	trisaccharide					1035:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide	997:1047	Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans, and NMR-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necessary for synthesis, assembly, and membrane translocation of a lipid-linked O-glycan, as well as its structure, which consists of a β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc trisaccharide.					
31350337	2	69	theme	human	293:297	arg1	infections					299:308	life-threatening human infections	276:308	life-threatening human infections	276:308	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	1	70	theme	Burkholderia	154:165	arg1	genus					167:171	The Burkholderia genus	150:171	The Burkholderia genus	150:171	The Burkholderia genus encompasses many Gram-negative bacteria living in the rhizosphere.					
31350337	5	71	theme	O-glycosylation	1319:1333	arg1	absence					1300:1306	absence	1300:1306	absence of protein O-glycosylation	1300:1333	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	3	72	theme	Burkholderia	408:419	arg1	glycosylation					450:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation	408:462	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	2	73	theme	life-threatening	276:291	arg1	infections					299:308	life-threatening human infections	276:308	life-threatening human infections	276:308	Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins.					
31350337	5	74	theme	larva	1431:1435	arg1	model					1447:1451	a Galleria mellonella larva infection model	1409:1451	a Galleria mellonella larva infection model	1409:1451	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
31350337	3	75	theme	potential	603:611	arg1	targets					625:631	potential therapeutic targets	603:631	potential therapeutic targets	603:631	Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets.					
31350337	7	76	theme	immunotherapy	1809:1821	arg1	approaches					1823:1832	immunotherapy approaches	1809:1832	immunotherapy approaches	1809:1832	Together, these results highlight the importance of general protein O-glycosylation in the biology of the Burkholderia genus and its potential as a target for inhibition or immunotherapy approaches to control Burkholderia infections.					
31350337	5	77	theme	Burkholderia	1201:1212	arg1	species					1214:1220	the Burkholderia species	1197:1220	the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei	1197:1272	We demonstrate that the ogc cluster is conserved in the Burkholderia genus, and we confirm the production of glycoproteins with similar glycans in the Burkholderia species: B. thailandensis, B. gladioli, and B. pseudomallei Furthermore, we show that absence of protein O-glycosylation severely affects bacterial fitness and accelerates bacterial clearance in a Galleria mellonella larva infection model.					
30923042	11	0	dep	caeca	1550:1554	arg1	mucins					1577:1582	mucins	1577:1582	mucins	1577:1582	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	10	1	theme	geographical	1378:1389	arg1	origin					1391:1396	geographical origin	1378:1396	geographical origin	1378:1396	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	4	2	from	location	391:398	arg1	O-glycome					367:375	The Atlantic salmon O-glycome	347:375	The Atlantic salmon O-glycome from a single location	347:398	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	9	3	theme	salmon	1165:1170	arg1	populations					1172:1182	Atlantic salmon populations	1156:1182	Atlantic salmon populations	1156:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	4	4	theme	single	384:389	arg1	location					391:398	a single location	382:398	a single location	382:398	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	9	5	from	Swedish	1134:1140	arg1	related					1197:1203	related	1197:1203	related	1197:1203	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	5	from	Swedish	1134:1140	arg1	repertoires					1117:1127	The skin mucin glycan repertoires	1095:1127	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations	1095:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	5	6	theme	interindividual	622:636	arg1	variation					638:646	low interindividual variation	618:646	low interindividual variation among Atlantic salmon	618:668	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	5	6	theme	interindividual	622:636	arg1	concern					679:685	a concern	677:685	a concern	677:685	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	10	7	theme	pyloric	1407:1413	arg1	groups					1443:1448	pyloric cecal and distal intestinal groups	1407:1448	pyloric cecal and distal intestinal groups	1407:1448	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	11	8	theme	pyloric	1542:1548	arg1	caeca					1550:1554	Tasmanian pyloric caeca	1532:1554	Tasmanian pyloric caeca	1532:1554	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	14	9	theme	host-pathogen	1903:1915	arg1	interactions					1917:1928	host-pathogen interactions	1903:1928	host-pathogen interactions	1903:1928	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	4	10	theme	salmon	360:365	arg1	O-glycome					367:375	The Atlantic salmon O-glycome	347:375	The Atlantic salmon O-glycome from a single location	347:398	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	13	11	theme	interindividual	1763:1777	arg1	variation					1779:1787	Low interindividual variation	1759:1787	Low interindividual variation	1759:1787	Low interindividual variation was confirmed within each cohort.					
30923042	12	12	theme	Tasmanian	1621:1629	arg1	salmon					1640:1645	Tasmanian Atlantic salmon	1621:1645	Tasmanian Atlantic salmon mucins	1621:1652	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	2	13	gly	O-glycosylated	214:227	arg1	mucins					229:234	highly O-glycosylated mucins	207:234	highly O-glycosylated mucins	207:234	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	15	14	theme	inter-region	2171:2182	arg1	breeding					2184:2191	inter-region breeding	2171:2191	inter-region breeding	2171:2191	The results could make it possible to predict potential vulnerabilities to diseases and suggest that inter-region breeding may increase the glycan diversity.					
30923042	3	15	theme	Mucin	285:289	arg1	glycans					291:297	Mucin glycans	285:297	Mucin glycans	285:297	Mucin glycans govern pathogen adhesion, growth and virulence.					
30923042	11	16	from	caeca	1550:1554	arg1	abundant					1520:1527	abundant	1520:1527	abundant	1520:1527	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	11	17	theme	Tasmanian	1532:1540	arg1	caeca					1550:1554	Tasmanian pyloric caeca	1532:1554	Tasmanian pyloric caeca	1532:1554	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	5	18	theme	population-based	516:531	arg1	defense					533:539	a population-based defense	514:539	a population-based defense	514:539	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	12	19	theme	gastrointestinal	1708:1723	arg1	tract					1725:1729	the gastrointestinal tract	1704:1729	the gastrointestinal tract	1704:1729	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	11	20	from	abundant	1520:1527	arg1	caeca					1550:1554	Tasmanian pyloric caeca	1532:1554	Tasmanian pyloric caeca	1532:1554	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	11	20	from	abundant	1520:1527	arg1	intestine					1567:1575	distal intestine	1560:1575	distal intestine	1560:1575	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	10	21	theme	intestinal	1432:1441	arg1	groups					1443:1448	pyloric cecal and distal intestinal groups	1407:1448	pyloric cecal and distal intestinal groups	1407:1448	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	9	22	from	Norwegian	1146:1154	arg1	related					1197:1203	related	1197:1203	related	1197:1203	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	22	from	Norwegian	1146:1154	arg1	repertoires					1117:1127	The skin mucin glycan repertoires	1095:1127	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations	1095:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	0	23	theme	Salmo	60:64	arg1	salmon					52:57	Atlantic salmon	43:57	Atlantic salmon	43:57	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	0	23	theme	Salmo	60:64	arg1	salar					66:70	Salmo salar	60:70	Salmo salar	60:70	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	0	24	from	Effects	0:6	arg1	salmon					52:57	Atlantic salmon	43:57	Atlantic salmon	43:57	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	0	24	from	Effects	0:6	arg1	salar					66:70	Salmo salar	60:70	Salmo salar	60:70	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	11	25	theme	Fucosylated	1487:1497	arg1	structures					1499:1508	Fucosylated structures	1487:1508	Fucosylated structures	1487:1508	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	14	26	from	differences	1956:1966	arg1	resistance					2001:2010	resistance	2001:2010	resistance to diseases	2001:2022	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	8	27	theme	geographical	1014:1025	arg1	region					1027:1032	a geographical region	1012:1032	a geographical region	1012:1032	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	0	28	theme	O-Glycan	79:86	arg1	Repertoire					88:97	O-Glycan Repertoire	79:97	O-Glycan Repertoire	79:97	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	12	29	theme	O-glycan	1664:1671	arg1	structures					1673:1682	more O-glycan structures	1659:1682	more O-glycan structures	1659:1682	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	9	30	with	related	1197:1203	arg1	differences					1273:1283	differences	1273:1283	differences in glycan size and composition	1273:1314	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	31	dep	Swedish	1134:1140	arg1	populations					1172:1182	Atlantic salmon populations	1156:1182	Atlantic salmon populations	1156:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	0	32	theme	Size	11:14	arg1	Effects					0:6	Effects	0:6	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar,	0:71	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	12	33	theme	salmon	1640:1645	arg1	mucins					1647:1652	Tasmanian Atlantic salmon mucins	1621:1652	Tasmanian Atlantic salmon mucins	1621:1652	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	0	34	theme	Geographical	20:31	arg1	Origin					33:38	Geographical Origin	20:38	Geographical Origin	20:38	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	8	35	theme	fish	1055:1058	arg1	size					1043:1046	the size	1039:1046	the size of the fish	1039:1058	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	9	36	theme	skin	1099:1102	arg1	related					1197:1203	related	1197:1203	related	1197:1203	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	36	theme	skin	1099:1102	arg1	repertoires					1117:1127	The skin mucin glycan repertoires	1095:1127	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations	1095:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	8	37	theme	skin	1069:1072	arg1	glycosylation					1080:1092	skin mucin glycosylation	1069:1092	skin mucin glycosylation	1069:1092	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	14	38	theme	mucin	1978:1982	arg1	glycosylation					1984:1996	salmon mucin glycosylation	1971:1996	salmon mucin glycosylation	1971:1996	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	2	39	theme	mucus	182:186	arg1	contact					249:255	the first contact	239:255	the first contact between pathogens and fish	239:282	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	2	39	theme	mucus	182:186	arg1	layer					188:192	The mucus layer	178:192	The mucus layer comprised of highly O-glycosylated mucins	178:234	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	6	40	theme	salmon	735:740	arg1	O-glycome					710:718	the O-glycome	706:718	the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania)	706:833	Here, we analyzed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry.					
30923042	7	41	theme	O-glycome	900:908	arg1	salmon					893:898	the known Atlantic salmon O-glycome	874:908	the known Atlantic salmon O-glycome by 60% to 169 identified structures	874:944	This expanded the known Atlantic salmon O-glycome by 60% to 169 identified structures.					
30923042	7	42	theme	Atlantic	884:891	arg1	salmon					893:898	the known Atlantic salmon O-glycome	874:908	the known Atlantic salmon O-glycome by 60% to 169 identified structures	874:944	This expanded the known Atlantic salmon O-glycome by 60% to 169 identified structures.					
30923042	14	43	theme	population	1945:1954	arg1	differences					1956:1966	population differences	1945:1966	population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains	1945:2067	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	5	44	theme	Atlantic	654:661	arg1	salmon					663:668	Atlantic salmon	654:668	Atlantic salmon	654:668	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	10	45	theme	mucin	1330:1334	arg1	repertoire					1343:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	14	46	dep	identifying	1862:1872	arg1	understanding					1931:1943	understanding	1931:1943	understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains	1931:2067	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	8	47	theme	mucin	951:955	arg1	O-glycosylation					957:971	The mucin O-glycosylation	947:971	The mucin O-glycosylation	947:971	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	8	47	theme	mucin	951:955	arg1	stable					988:993	stable	988:993	stable	988:993	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	11	48	theme	distal	1560:1565	arg1	intestine					1567:1575	distal intestine	1560:1575	distal intestine	1560:1575	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	9	49	theme	Atlantic	1156:1163	arg1	populations					1172:1182	Atlantic salmon populations	1156:1182	Atlantic salmon populations	1156:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	5	50	theme	low	618:620	arg1	variation					638:646	low interindividual variation	618:646	low interindividual variation among Atlantic salmon	618:668	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	5	50	theme	low	618:620	arg1	concern					679:685	a concern	677:685	a concern	677:685	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	15	51	theme	glycan	2210:2215	arg1	diversity					2217:2225	the glycan diversity	2206:2225	the glycan diversity	2206:2225	The results could make it possible to predict potential vulnerabilities to diseases and suggest that inter-region breeding may increase the glycan diversity.					
30923042	4	52	theme	Atlantic	351:358	arg1	O-glycome					367:375	The Atlantic salmon O-glycome	347:375	The Atlantic salmon O-glycome from a single location	347:398	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	12	53	contain	have	1654:1657	arg2	structures					1673:1682	more O-glycan structures	1659:1682	more O-glycan structures	1659:1682	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	12	53	contain	have	1654:1657	arg1	mucins					1647:1652	Tasmanian Atlantic salmon mucins	1621:1652	Tasmanian Atlantic salmon mucins	1621:1652	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	6	54	theme	various	771:777	arg1	conditions					779:788	various conditions	771:788	various conditions	771:788	Here, we analyzed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry.					
30923042	1	55	theme	Salmonid	159:166	arg1	industry					168:175	the Salmonid industry	155:175	the Salmonid industry	155:175	Diseases cause ethical concerns and economic losses in the Salmonid industry.					
30923042	12	56	theme	Swedish	1745:1751	arg1	fish					1753:1756	Swedish fish	1745:1756	Swedish fish	1745:1756	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	13	57	theme	Low	1759:1761	arg1	variation					1779:1787	Low interindividual variation	1759:1787	Low interindividual variation	1759:1787	Low interindividual variation was confirmed within each cohort.					
30923042	11	58	from	intestine	1567:1575	arg1	abundant					1520:1527	abundant	1520:1527	abundant	1520:1527	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	4	59	theme	interindividual	431:445	arg1	low					461:463	low	461:463	low	461:463	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	4	59	theme	interindividual	431:445	arg1	variation					447:455	the interindividual variation	427:455	the interindividual variation	427:455	The Atlantic salmon O-glycome from a single location has been characterized and the interindividual variation was low.					
30923042	9	60	theme	Tasmanian	1219:1227	arg1	ones					1229:1232	Tasmanian ones	1219:1232	Tasmanian ones	1219:1232	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	2	61	theme	O-glycosylated	214:227	arg1	mucins					229:234	highly O-glycosylated mucins	207:234	highly O-glycosylated mucins	207:234	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	5	62	theme	interindividual	474:488	arg1	variation					490:498	interindividual variation	474:498	interindividual variation	474:498	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	9	63	from	differences	1273:1283	arg1	composition					1304:1314	composition	1304:1314	composition	1304:1314	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	63	from	differences	1273:1283	arg1	size					1295:1298	glycan size	1288:1298	glycan size	1288:1298	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	5	64	theme	entire	556:561	arg1	population					563:572	the entire population	552:572	the entire population	552:572	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	15	65	theme	potential	2116:2124	arg1	vulnerabilities					2126:2140	potential vulnerabilities	2116:2140	potential vulnerabilities to diseases	2116:2152	The results could make it possible to predict potential vulnerabilities to diseases and suggest that inter-region breeding may increase the glycan diversity.					
30923042	2	66	theme	first	243:247	arg1	contact					249:255	the first contact	239:255	the first contact between pathogens and fish	239:282	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	2	66	theme	first	243:247	arg1	layer					188:192	The mucus layer	178:192	The mucus layer comprised of highly O-glycosylated mucins	178:234	The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish.					
30923042	9	67	theme	glycan	1110:1115	arg1	related					1197:1203	related	1197:1203	related	1197:1203	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	67	theme	glycan	1110:1115	arg1	repertoires					1117:1127	The skin mucin glycan repertoires	1095:1127	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations	1095:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	14	68	theme	importance	1888:1897	arg1	structures					1874:1883	structures	1874:1883	structures of importance	1874:1897	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	3	69	theme	pathogen	306:313	arg1	adhesion					315:322	pathogen adhesion	306:322	pathogen adhesion	306:322	Mucin glycans govern pathogen adhesion, growth and virulence.					
30923042	10	70	theme	distal	1425:1430	arg1	groups					1443:1448	pyloric cecal and distal intestinal groups	1407:1448	pyloric cecal and distal intestinal groups	1407:1448	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	5	71	theme	single	600:605	arg1	infection					607:615	a single infection	598:615	a single infection	598:615	Because interindividual variation is considered a population-based defense, hindering the entire population from being wiped out by a single infection, low interindividual variation among Atlantic salmon may be a concern.					
30923042	10	72	theme	cecal	1415:1419	arg1	groups					1443:1448	pyloric cecal and distal intestinal groups	1407:1448	pyloric cecal and distal intestinal groups	1407:1448	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	1	73	dep	ethical	115:121	arg1	concerns					123:130	concerns	123:130	concerns	123:130	Diseases cause ethical concerns and economic losses in the Salmonid industry.					
30923042	1	73	dep	ethical	115:121	arg1	losses					145:150	losses	145:150	losses	145:150	Diseases cause ethical concerns and economic losses in the Salmonid industry.					
30923042	9	74	theme	glycan	1288:1293	arg1	size					1295:1298	glycan size	1288:1298	glycan size	1288:1298	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	7	75	theme	identified	924:933	arg1	structures					935:944	169 identified structures	920:944	169 identified structures	920:944	This expanded the known Atlantic salmon O-glycome by 60% to 169 identified structures.					
30923042	10	76	theme	fish	1476:1479	arg1	size					1481:1484	fish size	1476:1484	fish size	1476:1484	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	0	77	theme	Origin	33:38	arg1	Effects					0:6	Effects	0:6	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar,	0:71	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	6	78	theme	mass	841:844	arg1	spectrometry					846:857	mass spectrometry	841:857	mass spectrometry	841:857	Here, we analyzed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry.					
30923042	12	79	theme	Atlantic	1631:1638	arg1	salmon					1640:1645	Tasmanian Atlantic salmon	1621:1645	Tasmanian Atlantic salmon mucins	1621:1652	Overall, Tasmanian Atlantic salmon mucins have more O-glycan structures in skin but less in the gastrointestinal tract compared with Swedish fish.					
30923042	10	80	theme	internal	1321:1328	arg1	repertoire					1343:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30923042	0	81	theme	Atlantic	43:50	arg1	salmon					52:57	Atlantic salmon	43:57	Atlantic salmon	43:57	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	0	81	theme	Atlantic	43:50	arg1	salar					66:70	Salmo salar	60:70	Salmo salar	60:70	Effects of Size and Geographical Origin on Atlantic salmon, Salmo salar, Mucin O-Glycan Repertoire.					
30923042	8	82	theme	mucin	1074:1078	arg1	glycosylation					1080:1092	skin mucin glycosylation	1069:1092	skin mucin glycosylation	1069:1092	The mucin O-glycosylation was relatively stable over time within a geographical region, but the size of the fish affected skin mucin glycosylation.					
30923042	14	83	theme	glycosylation	1984:1996	arg1	differences					1956:1966	population differences	1945:1966	population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains	1945:2067	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	9	84	theme	mucin	1104:1108	arg1	related					1197:1203	related	1197:1203	related	1197:1203	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	9	84	theme	mucin	1104:1108	arg1	repertoires					1117:1127	The skin mucin glycan repertoires	1095:1127	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations	1095:1182	The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared with Tasmanian ones, regardless of size and salinity, with differences in glycan size and composition.					
30923042	14	85	theme	salmon	1971:1976	arg1	glycosylation					1984:1996	salmon mucin glycosylation	1971:1996	salmon mucin glycosylation	1971:1996	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	6	86	from	cohorts	751:757	arg1	O-glycome					710:718	the O-glycome	706:718	the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania)	706:833	Here, we analyzed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry.					
30923042	11	87	theme	Swedish	1598:1604	arg1	ones					1606:1609	Swedish ones	1598:1609	Swedish ones	1598:1609	Fucosylated structures were more abundant in Tasmanian pyloric caeca and distal intestine mucins compared with Swedish ones.					
30923042	14	88	theme	strains	2061:2067	arg1	selection					2048:2056	selection	2048:2056	selection	2048:2056	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	14	88	theme	strains	2061:2067	arg1	breeding					2035:2042	breeding	2035:2042	breeding	2035:2042	The results can serve as a library for identifying structures of importance for host-pathogen interactions, understanding population differences of salmon mucin glycosylation in resistance to diseases and during breeding and selection of strains.					
30923042	6	89	theme	Atlantic	726:733	arg1	salmon					735:740	25 Atlantic salmon	723:740	25 Atlantic salmon	723:740	Here, we analyzed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry.					
30923042	7	90	theme	known	878:882	arg1	salmon					893:898	the known Atlantic salmon O-glycome	874:908	the known Atlantic salmon O-glycome by 60% to 169 identified structures	874:944	This expanded the known Atlantic salmon O-glycome by 60% to 169 identified structures.					
30923042	10	91	theme	glycan	1336:1341	arg1	repertoire					1343:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire	1317:1352	The internal mucin glycan repertoire also clustered based on geographical origin and into pyloric cecal and distal intestinal groups, regardless of cohort and fish size.					
30395580	4	0	contain	has	676:678	arg1	protein					646:652	The recombinant protein	630:652	The recombinant protein produced in A. oryzae	630:674	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	0	contain	has	676:678	arg2	mass					692:695	a molecular mass	680:695	a molecular mass of 75 kDa	680:705	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	0	contain	has	676:678	arg2	pI					710:711	a pI	708:711	a pI of 4.2	708:718	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	3	1	theme	other	542:546	arg1	members					548:554	other members	542:554	other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	542:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	8	2	theme	site	1183:1186	arg1	topology					1188:1195	the T1-Cu active site topology	1166:1195	the T1-Cu active site topology	1166:1195	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	5	3	theme	phenolic	802:809	arg1	pocket					829:834	the phenolic substrate binding pocket	798:834	the phenolic substrate binding pocket blocked	798:842	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	8	4	from	differences	1151:1161	arg1	topology					1188:1195	the T1-Cu active site topology	1166:1195	the T1-Cu active site topology	1166:1195	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	8	4	from	differences	1151:1161	arg1	motifs					1207:1212	polar motifs	1201:1212	polar motifs	1201:1212	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	3	5	theme	CAZy	563:566	arg1	AA1_3					575:579	the CAZy family AA1_3	559:579	the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	559:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	5	6	theme	binding	821:827	arg1	pocket					829:834	the phenolic substrate binding pocket	798:834	the phenolic substrate binding pocket blocked	798:842	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	8	7	theme	Notable	1143:1149	arg1	differences					1151:1161	Notable differences	1143:1161	Notable differences in the T1-Cu active site topology and polar motifs	1143:1212	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	10	8	theme	extensive	1552:1560	arg1	interactions					1562:1573	extensive interactions	1552:1573	extensive interactions with the protein	1552:1590	The N-glycosylation sites are surprisingly conserved among asco-laccases and in most cases the glycan displays extensive interactions with the protein.					
30395580	1	9	theme	catalytic	211:219	arg1	mechanism					221:229	the generic catalytic mechanism	199:229	the generic catalytic mechanism	199:229	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	12	10	theme	particular	1905:1914	arg1	function-in					1893:1903	laccase function-in particular	1885:1914	laccase function-in particular	1885:1914	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	8	11	theme	molecular	1220:1228	arg1	evolution					1230:1238	molecular evolution	1220:1238	molecular evolution to serve different functional roles	1220:1274	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	2	12	theme	other	241:245	arg1	features					247:254	other features	241:254	other features such as surface properties and residues located away from the catalytic centres	241:334	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	12	theme	other	241:245	arg1	residues					287:294	residues	287:294	residues	287:294	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	12	theme	other	241:245	arg1	properties					272:281	surface properties	264:281	surface properties	264:281	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	8	13	theme	T1-Cu	1170:1174	arg1	topology					1188:1195	the T1-Cu active site topology	1166:1195	the T1-Cu active site topology	1166:1195	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	3	14	theme	AA1_3	575:579	arg1	members					548:554	other members	542:554	other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	542:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	8	15	theme	different	1249:1257	arg1	roles					1270:1274	different functional roles	1249:1274	different functional roles	1249:1274	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	1	16	theme	biological	100:109	arg1	origins					111:117	different biological origins	90:117	different biological origins	90:117	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	11	17	theme	structure	1703:1711	arg1	part					1678:1681	an integral part	1666:1681	an integral part of the asco-laccase structure	1666:1711	In particular, the glycans at Asn88 and Asn210 appear to have evolved as an integral part of the asco-laccase structure.					
30395580	2	18	theme	surface	264:270	arg1	properties					272:281	surface properties	264:281	surface properties	264:281	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	6	19	theme	canonical	950:958	arg1	fold					960:963	the canonical fold	946:963	the canonical fold of fungal laccases as well as the features specific for the asco-laccases	946:1037	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	2	20	contain	have	345:348	arg1	features					247:254	other features	241:254	other features such as surface properties and residues located away from the catalytic centres	241:334	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	20	contain	have	345:348	arg2	impact					350:355	impact	350:355	impact	350:355	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	20	contain	have	345:348	arg1	residues					287:294	residues	287:294	residues	287:294	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	20	contain	have	345:348	arg1	properties					272:281	surface properties	264:281	surface properties	264:281	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	21	from	centres	328:334	arg1	located					296:302	located	296:302	located	296:302	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	2	22	theme	located	296:302	arg1	properties					272:281	surface properties	264:281	surface properties	264:281	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	4	23	theme	kDa	737:739	arg1	glycans					750:756	13.5 kDa N-linked glycans	732:756	13.5 kDa N-linked glycans	732:756	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	11	24	from	Asn88	1623:1627	arg1	glycans					1612:1618	the glycans	1608:1618	the glycans at Asn88 and Asn210	1608:1638	In particular, the glycans at Asn88 and Asn210 appear to have evolved as an integral part of the asco-laccase structure.					
30395580	3	25	from	ascomycetes	617:627	arg1	laccases					603:610	fungal laccases	596:610	fungal laccases from ascomycetes	596:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	9	26	theme	few	1282:1284	arg1	residues					1294:1301	Very few surface residues	1277:1301	Very few surface residues	1277:1301	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	1	27	theme	different	90:98	arg1	origins					111:117	different biological origins	90:117	different biological origins	90:117	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	11	28	from	Asn210	1633:1638	arg1	glycans					1612:1618	the glycans	1608:1618	the glycans at Asn88 and Asn210	1608:1638	In particular, the glycans at Asn88 and Asn210 appear to have evolved as an integral part of the asco-laccase structure.					
30395580	5	29	with	dimer	787:791	arg1	pocket					829:834	the phenolic substrate binding pocket	798:834	the phenolic substrate binding pocket blocked	798:842	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	4	30	theme	4.2	716:718	arg1	mass					692:695	a molecular mass	680:695	a molecular mass of 75 kDa	680:705	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	30	theme	4.2	716:718	arg1	pI					710:711	a pI	708:711	a pI of 4.2	708:718	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	0	31	theme	structural	14:23	arg1	analysis					25:32	A comparative structural analysis	0:32	A comparative structural analysis of the surface properties of asco-laccases	0:75	A comparative structural analysis of the surface properties of asco-laccases.					
30395580	1	32	theme	origins	111:117	arg1	Laccases					78:85	Laccases	78:85	Laccases of different biological origins	78:117	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	6	33	theme	laccases	975:982	arg1	features					999:1006	the features	995:1006	the canonical fold of fungal laccases as well as the features specific for the asco-laccases	946:1037	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	6	33	theme	laccases	975:982	arg1	fold					960:963	the canonical fold	946:963	the canonical fold of fungal laccases as well as the features specific for the asco-laccases	946:1037	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	12	34	contain	have	1864:1867	arg2	implication					1869:1879	implication	1869:1879	implication for laccase function-in particular towards large substrates	1869:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	12	34	contain	have	1864:1867	arg1	asco-laccases					1840:1852	the asco-laccases	1836:1852	the asco-laccases which may have implication for laccase function-in particular towards large substrates	1836:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	3	35	with	comparison	526:535	arg1	members					548:554	other members	542:554	other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	542:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	12	36	theme	surface	1825:1831	arg1	part					1813:1816	a distinct part	1802:1816	a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates	1802:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	3	37	dep	Myceliophthora	431:444	arg1	thermophila					446:456	Myceliophthora thermophila	431:456	Myceliophthora thermophila (MtL)	431:462	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	10	38	with	interactions	1562:1573	arg1	protein					1584:1590	the protein	1580:1590	the protein	1580:1590	The N-glycosylation sites are surprisingly conserved among asco-laccases and in most cases the glycan displays extensive interactions with the protein.					
30395580	4	39	link	N-linked	741:748	arg1	glycans					750:756	13.5 kDa N-linked glycans	732:756	13.5 kDa N-linked glycans	732:756	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	3	40	theme	crystal	397:403	arg1	structure					405:413	the crystal structure	393:413	the crystal structure of laccase	393:424	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	3	41	theme	1.62	483:486	arg1	resolution					469:478	a resolution	467:478	a resolution of 1.62 Å	467:488	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	0	42	theme	surface	41:47	arg1	properties					49:58	the surface properties	37:58	the surface properties of asco-laccases	37:75	A comparative structural analysis of the surface properties of asco-laccases.					
30395580	12	43	theme	distinct	1804:1811	arg1	part					1813:1816	a distinct part	1802:1816	a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates	1802:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	4	44	dep	A.	666:667	arg1	oryzae					669:674	A. oryzae	666:674	A. oryzae	666:674	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	12	45	theme	carbohydrates	1744:1756	arg1	distribution					1724:1735	An uneven distribution	1714:1735	An uneven distribution of the carbohydrates around the enzyme	1714:1774	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	10	46	theme	most	1521:1524	arg1	cases					1526:1530	most cases	1521:1530	most cases	1521:1530	The N-glycosylation sites are surprisingly conserved among asco-laccases and in most cases the glycan displays extensive interactions with the protein.					
30395580	11	47	theme	integral	1669:1676	arg1	part					1678:1681	an integral part	1666:1681	an integral part of the asco-laccase structure	1666:1711	In particular, the glycans at Asn88 and Asn210 appear to have evolved as an integral part of the asco-laccase structure.					
30395580	0	48	theme	properties	49:58	arg1	analysis					25:32	A comparative structural analysis	0:32	A comparative structural analysis of the surface properties of asco-laccases	0:75	A comparative structural analysis of the surface properties of asco-laccases.					
30395580	2	49	theme	enzyme	360:365	arg1	function					367:374	enzyme function	360:374	enzyme function	360:374	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	12	50	theme	uneven	1717:1722	arg1	distribution					1724:1735	An uneven distribution	1714:1735	An uneven distribution of the carbohydrates around the enzyme	1714:1774	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	6	51	theme	specific	1008:1015	arg1	features					999:1006	the features	995:1006	the canonical fold of fungal laccases as well as the features specific for the asco-laccases	946:1037	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	3	52	theme	structural	515:524	arg1	comparison					526:535	a thorough structural comparison	504:535	a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	504:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	9	53	located	located	1411:1417	arg2	residues					1358:1365	residues	1358:1365	residues that interact with the N-glycans and/or are located at domain interfaces	1358:1438	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	9	53	located	located	1411:1417	arg1	interfaces					1429:1438	domain interfaces	1422:1438	domain interfaces	1422:1438	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	8	54	theme	active	1176:1181	arg1	topology					1188:1195	the T1-Cu active site topology	1166:1195	the T1-Cu active site topology	1166:1195	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	5	55	theme	substrate	811:819	arg1	pocket					829:834	the phenolic substrate binding pocket	798:834	the phenolic substrate binding pocket blocked	798:842	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	10	56	gly	N-glycosylation	1445:1459	arg2	sites					1461:1465	The N-glycosylation sites	1441:1465	The N-glycosylation sites	1441:1465	The N-glycosylation sites are surprisingly conserved among asco-laccases and in most cases the glycan displays extensive interactions with the protein.					
30395580	1	57	theme	generic	203:209	arg1	mechanism					221:229	the generic catalytic mechanism	199:229	the generic catalytic mechanism	199:229	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	2	58	theme	catalytic	318:326	arg1	centres					328:334	the catalytic centres	314:334	the catalytic centres	314:334	However, other features such as surface properties and residues located away from the catalytic centres may also have impact on enzyme function.					
30395580	8	59	theme	polar	1201:1205	arg1	motifs					1207:1212	polar motifs	1201:1212	polar motifs	1201:1212	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	9	60	theme	domain	1422:1427	arg1	interfaces					1429:1438	domain interfaces	1422:1438	domain interfaces	1422:1438	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	4	61	theme	recombinant	634:644	arg1	protein					646:652	The recombinant protein	630:652	The recombinant protein produced in A. oryzae	630:674	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	3	62	theme	family	568:573	arg1	AA1_3					575:579	the CAZy family AA1_3	559:579	the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	559:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	4	63	contain	carries	724:730	arg1	protein					646:652	The recombinant protein	630:652	The recombinant protein produced in A. oryzae	630:674	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	63	contain	carries	724:730	arg2	glycans					750:756	13.5 kDa N-linked glycans	732:756	13.5 kDa N-linked glycans	732:756	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	5	64	theme	active	865:870	arg1	monomeric					894:902	monomeric	894:902	monomeric	894:902	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	5	64	theme	active	865:870	arg1	form					872:875	the active form	861:875	the active form of the enzyme	861:889	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	4	65	theme	kDa	703:705	arg1	mass					692:695	a molecular mass	680:695	a molecular mass of 75 kDa	680:705	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	65	theme	kDa	703:705	arg1	pI					710:711	a pI	708:711	a pI of 4.2	708:718	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	0	66	theme	asco-laccases	63:75	arg1	properties					49:58	the surface properties	37:58	the surface properties of asco-laccases	37:75	A comparative structural analysis of the surface properties of asco-laccases.					
30395580	3	67	theme	fungal	596:601	arg1	laccases					603:610	fungal laccases	596:610	fungal laccases from ascomycetes	596:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	8	68	theme	functional	1259:1268	arg1	roles					1270:1274	different functional roles	1249:1274	different functional roles	1249:1274	Notable differences in the T1-Cu active site topology and polar motifs imply molecular evolution to serve different functional roles.					
30395580	11	69	theme	asco-laccase	1690:1701	arg1	structure					1703:1711	the asco-laccase structure	1686:1711	the asco-laccase structure	1686:1711	In particular, the glycans at Asn88 and Asn210 appear to have evolved as an integral part of the asco-laccase structure.					
30395580	4	70	theme	molecular	682:690	arg1	mass					692:695	a molecular mass	680:695	a molecular mass of 75 kDa	680:705	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	4	70	theme	molecular	682:690	arg1	pI					710:711	a pI	708:711	a pI of 4.2	708:718	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	12	71	theme	laccase	1885:1891	arg1	function-in					1893:1903	laccase function-in particular	1885:1914	laccase function-in particular	1885:1914	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	6	72	theme	MtL	918:920	arg1	structure					922:930	the MtL structure	914:930	the MtL structure	914:930	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	7	73	theme	taxonomic	1123:1131	arg1	subgroup					1133:1140	this taxonomic subgroup	1118:1140	this taxonomic subgroup	1118:1140	However, the structural comparisons also reveal significant variations within this taxonomic subgroup.					
30395580	12	74	theme	asco-laccases	1840:1852	arg1	surface					1825:1831	the surface	1821:1831	the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates	1821:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	4	75	theme	13.5	732:735	arg1	kDa					737:739	kDa	737:739	kDa	737:739	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	0	76	theme	comparative	2:12	arg1	analysis					25:32	A comparative structural analysis	0:32	A comparative structural analysis of the surface properties of asco-laccases	0:75	A comparative structural analysis of the surface properties of asco-laccases.					
30395580	7	77	theme	significant	1088:1098	arg1	variations					1100:1109	significant variations	1088:1109	significant variations within this taxonomic subgroup	1088:1140	However, the structural comparisons also reveal significant variations within this taxonomic subgroup.					
30395580	9	78	theme	surface	1286:1292	arg1	residues					1294:1301	Very few surface residues	1277:1301	Very few surface residues	1277:1301	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	5	79	theme	enzyme	884:889	arg1	monomeric					894:902	monomeric	894:902	monomeric	894:902	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	5	79	theme	enzyme	884:889	arg1	form					872:875	the active form	861:875	the active form of the enzyme	861:889	In the crystal, MtL forms a dimer with the phenolic substrate binding pocket blocked, suggesting that the active form of the enzyme is monomeric.					
30395580	10	80	theme	N-glycosylation	1445:1459	arg1	sites					1461:1465	The N-glycosylation sites	1441:1465	The N-glycosylation sites	1441:1465	The N-glycosylation sites are surprisingly conserved among asco-laccases and in most cases the glycan displays extensive interactions with the protein.					
30395580	1	81	theme	mechanism	221:229	arg1	fundamentals					183:194	fundamentals	183:194	fundamentals of the generic catalytic mechanism	183:229	Laccases of different biological origins have been widely investigated and these studies have elucidated fundamentals of the generic catalytic mechanism.					
30395580	6	82	theme	fungal	968:973	arg1	laccases					975:982	fungal laccases	968:982	fungal laccases	968:982	Overall, the MtL structure conforms with the canonical fold of fungal laccases as well as the features specific for the asco-laccases.					
30395580	3	83	theme	laccase	418:424	arg1	structure					405:413	the crystal structure	393:413	the crystal structure of laccase	393:424	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
30395580	7	84	theme	structural	1053:1062	arg1	comparisons					1064:1074	the structural comparisons	1049:1074	the structural comparisons	1049:1074	However, the structural comparisons also reveal significant variations within this taxonomic subgroup.					
30395580	9	85	from	interfaces	1429:1438	arg1	located					1411:1417	located	1411:1417	located	1411:1417	Very few surface residues are conserved and it is noticeable that they encompass residues that interact with the N-glycans and/or are located at domain interfaces.					
30395580	12	86	theme	unique	1781:1786	arg1	properties					1788:1797	unique properties	1781:1797	unique properties	1781:1797	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	4	87	theme	N-linked	741:748	arg1	glycans					750:756	13.5 kDa N-linked glycans	732:756	13.5 kDa N-linked glycans	732:756	The recombinant protein produced in A. oryzae has a molecular mass of 75 kDa, a pI of 4.2 and carries 13.5 kDa N-linked glycans.					
30395580	12	88	theme	large	1924:1928	arg1	substrates					1930:1939	large substrates	1924:1939	large substrates	1924:1939	An uneven distribution of the carbohydrates around the enzyme give unique properties to a distinct part of the surface of the asco-laccases which may have implication for laccase function-in particular towards large substrates.					
30395580	3	89	theme	thorough	506:513	arg1	comparison					526:535	a thorough structural comparison	504:535	a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes	504:627	Here we present the crystal structure of laccase from Myceliophthora thermophila (MtL) to a resolution of 1.62 Å together with a thorough structural comparison with other members of the CAZy family AA1_3 that comprises fungal laccases from ascomycetes.					
31117584	8	0	with	N-glycosylation	1891:1905	arg1	relevance					1943:1951	physiological and pathological relevance	1912:1951	physiological and pathological relevance	1912:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	1	1	attach	presence	334:341	arg2	structures					365:374	different N-glycan structures	346:374	different N-glycan structures	346:374	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	1	attach	presence	334:341	arg1	glycosite					397:405	a single specific glycosite	379:405	a single specific glycosite	379:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	2	gly	glycosite	397:405	arg2	glycosite					397:405	a single specific glycosite	379:405	a single specific glycosite	379:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	3	theme	common	221:226	arg1	PTMs					282:285	PTMs	282:285	PTMs	282:285	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	3	theme	common	221:226	arg1	modifications					267:279	the most common and complex protein post-translational modifications	212:279	the most common and complex protein post-translational modifications (PTMs)	212:286	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	4	theme	complex	232:238	arg1	PTMs					282:285	PTMs	282:285	PTMs	282:285	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	4	theme	complex	232:238	arg1	modifications					267:279	the most common and complex protein post-translational modifications	212:279	the most common and complex protein post-translational modifications (PTMs)	212:286	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	5	gly	N-glycopeptides	1102:1116	arg2	N-glycopeptides					1102:1116	5 405 and 1 081 intact N-glycopeptides	1079:1116	5 405 and 1 081 intact N-glycopeptides with putative linkage structures	1079:1149	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	7	6	from	combination	1505:1515	arg1	isomers					1492:1498	quadruple isomers	1482:1498	quadruple isomers from combination of two linages and two sequences	1482:1548	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	6	7	theme	diagnostic	1362:1371	arg1	ion					1382:1384	no less than one structure diagnostic fragment ion	1335:1384	no less than one structure diagnostic fragment ion for the N-glycan moieties	1335:1410	Among the 5 405 IDs, 837 were identified with no less than one structure diagnostic fragment ion for the N-glycan moieties.					
31117584	3	8	theme	N-glycans	673:681	arg1	characterization					647:662	large-scale characterization	635:662	large-scale characterization of these N-glycans at the composition level	635:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	5	9	theme	carcinoma	1034:1042	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	10	theme	N-glycosylation	1000:1014	arg1	characterization					955:970	benchmark characterization	945:970	benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells	945:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	4	11	theme	search	841:846	arg1	engine					848:853	the intact N-glycopeptide search engine	815:853	the intact N-glycopeptide search engine GPSeeker	815:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	4	11	theme	search	841:846	arg1	GPSeeker					855:862	GPSeeker	855:862	GPSeeker	855:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	1	12	contain	have	301:304	arg1	N-Glycosylation					182:196	N-Glycosylation	182:196	N-Glycosylation	182:196	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	12	contain	have	301:304	arg2	microheterogeneity					306:323	microheterogeneity	306:323	microheterogeneity	306:323	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	13	theme	putative	1123:1130	arg1	structures					1140:1149	putative linkage structures	1123:1149	putative linkage structures	1123:1149	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	8	14	theme	quantitative	1758:1769	arg1	pipeline					1800:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	7	15	theme	linkages	1451:1458	arg1	isomers					1428:1434	double isomers	1421:1434	double isomers of sialic acid linkages and fucose sequences	1421:1479	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	5	16	from	characterization	955:970	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	8	17	theme	N-glycoproteomics	1782:1798	arg1	pipeline					1800:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	4	18	theme	intact	819:824	arg1	engine					848:853	the intact N-glycopeptide search engine	815:853	the intact N-glycopeptide search engine GPSeeker	815:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	4	18	theme	intact	819:824	arg1	GPSeeker					855:862	GPSeeker	855:862	GPSeeker	855:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	7	19	theme	sialic	1439:1444	arg1	linkages					1451:1458	sialic acid linkages	1439:1458	sialic acid linkages	1439:1458	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	1	20	theme	specific	388:395	arg1	glycosite					397:405	a single specific glycosite	379:405	a single specific glycosite	379:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	8	21	theme	structural	1771:1780	arg1	pipeline					1800:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	4	22	theme	N-glycoproteomics	914:930	arg1	pipeline					932:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	0	23	theme	Expressed	127:135	arg1	N-Glycosylation					137:151	Differentially Expressed N-Glycosylation	112:151	Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	112:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	5	24	from	N-glycosylation	1000:1014	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	25	theme	isotopic	1187:1194	arg1	labeling					1205:1212	isotopic dimethyl labeling	1187:1212	isotopic dimethyl labeling	1187:1212	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	6	26	theme	the 5 405	1295:1303	arg1	IDs					1305:1307	the 5 405 IDs	1295:1307	the 5 405 IDs	1295:1307	Among the 5 405 IDs, 837 were identified with no less than one structure diagnostic fragment ion for the N-glycan moieties.					
31117584	5	27	theme	expressed	990:998	arg1	N-glycosylation					1000:1014	differentially expressed N-glycosylation	975:1014	differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells	975:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	7	28	located	observed	1679:1686	arg1	quantified N-glycosites					1710:1732	the 231 quantified N-glycosites	1702:1732	the 231 quantified N-glycosites	1702:1732	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	28	located	observed	1679:1686	arg1	183					1691:1693	183	1691:1693	183	1691:1693	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	28	located	observed	1679:1686	arg2	microheterogeneity					1613:1630	microheterogeneity	1613:1630	microheterogeneity with different differentially expressions	1613:1672	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	29	theme	sequences	1540:1548	arg1	combination					1505:1515	combination	1505:1515	combination of two linages and two sequences	1505:1548	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	2	30	theme	different	500:508	arg1	expressions					523:533	differential expressions	510:533	differential expressions	510:533	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	8	31	theme	qualitative	1842:1852	arg1	characterization					1871:1886	precise qualitative and quantitative characterization	1834:1886	precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance	1834:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	4	32	theme	quantitative	890:901	arg1	pipeline					932:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	3	33	from	level	702:706	arg1	characterization					647:662	large-scale characterization	635:662	large-scale characterization of these N-glycans at the composition level	635:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	3	33	from	level	702:706	arg1	N-glycans					673:681	these N-glycans	667:681	these N-glycans at the composition level	667:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	0	34	theme	Quantitative	17:28	arg1	N-Glycoproteomics					41:57	Quantitative Structural N-Glycoproteomics	17:57	Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	17:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	8	35	theme	quantitative	1858:1869	arg1	characterization					1871:1886	precise qualitative and quantitative characterization	1834:1886	precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance	1834:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	2	36	theme	monosaccharide	461:474	arg1	composition					476:486	exactly the same monosaccharide composition	444:486	exactly the same monosaccharide composition	444:486	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	1	37	from	glycosite	397:405	arg1	presence					334:341	the presence	330:341	the presence of different N-glycan structures at a single specific glycosite	330:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	38	theme	different	346:354	arg1	structures					365:374	different N-glycan structures	346:374	different N-glycan structures	346:374	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	39	theme	benchmark	945:953	arg1	characterization					955:970	benchmark characterization	945:970	benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells	945:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	40	theme	chromatography-tandem	1228:1248	arg1	LC-MS/MS					1269:1276	LC-MS/MS	1269:1276	LC-MS/MS	1269:1276	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	40	theme	chromatography-tandem	1228:1248	arg1	spectrometry					1255:1266	liquid chromatography-tandem mass spectrometry	1221:1266	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	2	41	theme	pathological	539:550	arg1	relevance					552:560	pathological relevance	539:560	pathological relevance	539:560	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	0	42	theme	Structure-Specific	73:90	arg1	Characterization					92:107	Site- and Structure-Specific Characterization	63:107	Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	63:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	0	43	theme	Site-	63:67	arg1	Characterization					92:107	Site- and Structure-Specific Characterization	63:107	Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	63:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	1	44	theme	structures	365:374	arg1	presence					334:341	the presence	330:341	the presence of different N-glycan structures at a single specific glycosite	330:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	45	theme	spectrometry	1255:1266	arg1	analysis					1279:1286	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	6	46	theme	less	1338:1341	arg1	ion					1382:1384	no less than one structure diagnostic fragment ion	1335:1384	no less than one structure diagnostic fragment ion for the N-glycan moieties	1335:1410	Among the 5 405 IDs, 837 were identified with no less than one structure diagnostic fragment ion for the N-glycan moieties.					
31117584	3	47	theme	large-scale	635:645	arg1	characterization					647:662	large-scale characterization	635:662	large-scale characterization of these N-glycans at the composition level	635:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	5	48	theme	2D	1218:1219	arg1	analysis					1279:1286	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	0	49	from	Carcinoma	171:179	arg1	Characterization					92:107	Site- and Structure-Specific Characterization	63:107	Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	63:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	7	50	theme	fucose	1464:1469	arg1	sequences					1471:1479	fucose sequences	1464:1479	fucose sequences	1464:1479	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	51	theme	quantified N-glycosites	1710:1732	arg1	quantified N-glycosites					1710:1732	the 231 quantified N-glycosites	1702:1732	the 231 quantified N-glycosites	1702:1732	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	51	theme	quantified N-glycosites	1710:1732	arg1	183					1691:1693	183	1691:1693	183	1691:1693	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	6	52	theme	N-glycan	1394:1401	arg1	moieties					1403:1410	the N-glycan moieties	1390:1410	the N-glycan moieties	1390:1410	Among the 5 405 IDs, 837 were identified with no less than one structure diagnostic fragment ion for the N-glycan moieties.					
31117584	3	53	from	successful	621:630	arg1	characterization					647:662	large-scale characterization	635:662	large-scale characterization of these N-glycans at the composition level	635:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	5	54	theme	relative	1056:1063	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	7	55	dep	quantified N-glycosites	1710:1732	arg1	out					1695:1697	out	1695:1697	out	1695:1697	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	5	56	theme	LO2	1068:1070	arg1	cells					1072:1076	LO2 cells	1068:1076	LO2 cells	1068:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	6	57	theme	fragment	1373:1380	arg1	ion					1382:1384	no less than one structure diagnostic fragment ion	1335:1384	no less than one structure diagnostic fragment ion for the N-glycan moieties	1335:1410	Among the 5 405 IDs, 837 were identified with no less than one structure diagnostic fragment ion for the N-glycan moieties.					
31117584	4	58	gly	N-glycopeptide	826:839	arg2	N-glycopeptide					826:839	the intact N-glycopeptide search engine	815:853	the intact N-glycopeptide search engine GPSeeker	815:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	1	59	theme	protein	240:246	arg1	PTMs					282:285	PTMs	282:285	PTMs	282:285	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	59	theme	protein	240:246	arg1	modifications					267:279	the most common and complex protein post-translational modifications	212:279	the most common and complex protein post-translational modifications (PTMs)	212:286	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	2	60	contain	have	439:442	arg2	composition					476:486	exactly the same monosaccharide composition	444:486	exactly the same monosaccharide composition	444:486	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	2	60	contain	have	439:442	arg2	expressions					523:533	differential expressions	510:533	differential expressions	510:533	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	2	60	contain	have	439:442	arg1	structures					424:433	These different structures	408:433	These different structures	408:433	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	5	61	theme	HepG2	1044:1048	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	0	62	from	Characterization	92:107	arg1	Carcinoma					171:179	Hepatocellular Carcinoma	156:179	Hepatocellular Carcinoma	156:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	7	63	theme	different	1637:1645	arg1	expressions					1662:1672	different differentially expressions	1637:1672	different differentially expressions	1637:1672	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	7	64	theme	double	1421:1426	arg1	isomers					1428:1434	double isomers	1421:1434	double isomers of sialic acid linkages and fucose sequences	1421:1479	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	3	65	theme	composition	690:700	arg1	level					702:706	the composition level	686:706	the composition level	686:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	5	66	from	cells	1050:1054	arg1	characterization					955:970	benchmark characterization	945:970	benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells	945:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	67	theme	intact	1095:1100	arg1	N-glycopeptides					1102:1116	5 405 and 1 081 intact N-glycopeptides	1079:1116	5 405 and 1 081 intact N-glycopeptides with putative linkage structures	1079:1149	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	8	68	theme	GPSeeker-centered	1740:1756	arg1	pipeline					1800:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline	1735:1807	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	4	69	theme	N-glycopeptide	826:839	arg1	engine					848:853	the intact N-glycopeptide search engine	815:853	the intact N-glycopeptide search engine GPSeeker	815:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	4	69	theme	N-glycopeptide	826:839	arg1	GPSeeker					855:862	GPSeeker	855:862	GPSeeker	855:862	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	0	70	from	N-Glycosylation	137:151	arg1	Carcinoma					171:179	Hepatocellular Carcinoma	156:179	Hepatocellular Carcinoma	156:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	4	71	theme	engine	848:853	arg1	development					800:810	our development	796:810	our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	796:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	7	72	theme	acid	1446:1449	arg1	linkages					1451:1458	sialic acid linkages	1439:1458	sialic acid linkages	1439:1458	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	3	73	theme	structure-level	713:727	arg1	identification					729:742	structure-level identification	713:742	structure-level identification	713:742	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	5	74	theme	hepatocellular	1019:1032	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	7	75	with	microheterogeneity	1613:1630	arg1	expressions					1662:1672	different differentially expressions	1637:1672	different differentially expressions	1637:1672	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	1	76	theme	single	381:386	arg1	glycosite					397:405	a single specific glycosite	379:405	a single specific glycosite	379:405	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	3	77	from	characterization	647:662	arg1	level					702:706	the composition level	686:706	the composition level	686:706	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	3	77	from	characterization	647:662	arg1	successful					621:630	successful	621:630	successful	621:630	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	0	78	theme	N-Glycosylation	137:151	arg1	Characterization					92:107	Site- and Structure-Specific Characterization	63:107	Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	63:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	4	79	theme	structural	903:912	arg1	pipeline					932:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	0	80	theme	Hepatocellular	156:169	arg1	Carcinoma					171:179	Hepatocellular Carcinoma	156:179	Hepatocellular Carcinoma	156:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	1	81	theme	post-translational	248:265	arg1	PTMs					282:285	PTMs	282:285	PTMs	282:285	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	81	theme	post-translational	248:265	arg1	modifications					267:279	the most common and complex protein post-translational modifications	212:279	the most common and complex protein post-translational modifications (PTMs)	212:286	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	7	82	gly	quantified N-glycosites	1710:1732	arg2	quantified N-glycosites					1710:1732	the 231 quantified N-glycosites	1702:1732	the 231 quantified N-glycosites	1702:1732	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	8	83	theme	precise	1834:1840	arg1	characterization					1871:1886	precise qualitative and quantitative characterization	1834:1886	precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance	1834:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	4	84	theme	pipeline	932:939	arg1	development					800:810	our development	796:810	our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	796:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	5	85	theme	linkage	1132:1138	arg1	structures					1140:1149	putative linkage structures	1123:1149	putative linkage structures	1123:1149	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	2	86	theme	differential	510:521	arg1	expressions					523:533	differential expressions	510:533	differential expressions	510:533	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	5	87	with	N-glycopeptides	1102:1116	arg1	structures					1140:1149	putative linkage structures	1123:1149	putative linkage structures	1123:1149	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	0	88	theme	Structural	30:39	arg1	N-Glycoproteomics					41:57	Quantitative Structural N-Glycoproteomics	17:57	Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma	17:179	GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.					
31117584	7	89	theme	linages	1524:1530	arg1	combination					1505:1515	combination	1505:1515	combination of two linages and two sequences	1505:1548	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	4	90	theme	GPSeeker-centered	872:888	arg1	pipeline					932:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	the GPSeeker-centered quantitative structural N-glycoproteomics pipeline	868:939	Here we report our development of the intact N-glycopeptide search engine GPSeeker and the GPSeeker-centered quantitative structural N-glycoproteomics pipeline.					
31117584	1	91	theme	modifications	267:279	arg1	one					205:207	one	205:207	one	205:207	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	91	theme	modifications	267:279	arg1	PTMs					282:285	PTMs	282:285	PTMs	282:285	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	1	91	theme	modifications	267:279	arg1	modifications					267:279	the most common and complex protein post-translational modifications	212:279	the most common and complex protein post-translational modifications (PTMs)	212:286	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	92	theme	liquid	1221:1226	arg1	LC-MS/MS					1269:1276	LC-MS/MS	1269:1276	LC-MS/MS	1269:1276	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	92	theme	liquid	1221:1226	arg1	spectrometry					1255:1266	liquid chromatography-tandem mass spectrometry	1221:1266	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	8	93	theme	N-glycosylation	1891:1905	arg1	characterization					1871:1886	precise qualitative and quantitative characterization	1834:1886	precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance	1834:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	2	94	theme	same	456:459	arg1	composition					476:486	exactly the same monosaccharide composition	444:486	exactly the same monosaccharide composition	444:486	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
31117584	1	95	theme	N-glycan	356:363	arg1	structures					365:374	different N-glycan structures	346:374	different N-glycan structures	346:374	N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite.					
31117584	5	96	theme	mass	1250:1253	arg1	LC-MS/MS					1269:1276	LC-MS/MS	1269:1276	LC-MS/MS	1269:1276	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	96	theme	mass	1250:1253	arg1	spectrometry					1255:1266	liquid chromatography-tandem mass spectrometry	1221:1266	2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1218:1286	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	8	97	theme	physiological	1912:1924	arg1	relevance					1943:1951	physiological and pathological relevance	1912:1951	physiological and pathological relevance	1912:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	5	98	gly	N-glycosylation	1000:1014	arg1	cells					1050:1054	hepatocellular carcinoma HepG2 cells	1019:1054	hepatocellular carcinoma HepG2 cells relative to LO2 cells	1019:1076	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	5	99	theme	dimethyl	1196:1203	arg1	labeling					1205:1212	isotopic dimethyl labeling	1187:1212	isotopic dimethyl labeling	1187:1212	In benchmark characterization of differentially expressed N-glycosylation in hepatocellular carcinoma HepG2 cells relative to LO2 cells, 5 405 and 1 081 intact N-glycopeptides with putative linkage structures were identified and quantified with isotopic dimethyl labeling and 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.					
31117584	7	100	theme	quadruple	1482:1490	arg1	isomers					1492:1498	quadruple isomers	1482:1498	quadruple isomers from combination of two linages and two sequences	1482:1548	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	8	101	theme	pathological	1930:1941	arg1	relevance					1943:1951	physiological and pathological relevance	1912:1951	physiological and pathological relevance	1912:1951	This GPSeeker-centered quantitative structural N-glycoproteomics pipeline can be widely applied to precise qualitative and quantitative characterization of N-glycosylation with physiological and pathological relevance.					
31117584	3	102	theme	spectrometry-based	568:585	arg1	N-glycoproteomics					587:603	Mass spectrometry-based N-glycoproteomics	563:603	Mass spectrometry-based N-glycoproteomics	563:603	Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans at the composition level, and structure-level identification and quantitation is urgently needed.					
31117584	7	103	theme	sequences	1471:1479	arg1	isomers					1428:1434	double isomers	1421:1434	double isomers of sialic acid linkages and fucose sequences	1421:1479	Besides double isomers of sialic acid linkages and fucose sequences, quadruple isomers from combination of two linages and two sequences were chromatographically separated and confidently identified; microheterogeneity with different differentially expressions were observed on 183 out of the 231 quantified N-glycosites.					
31117584	2	104	theme	different	414:422	arg1	structures					424:433	These different structures	408:433	These different structures	408:433	These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance.					
30327321	5	0	theme	X-ray	757:761	arg1	scattering					763:772	Small-angle X-ray scattering	745:772	Small-angle X-ray scattering	745:772	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	0	1	from	domain	15:20	arg1	collagen					54:61	collagen XVIII	54:67	collagen XVIII	54:67	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	5	2	dep	extended	803:810	arg1	flexible					813:820	flexible	813:820	flexible	813:820	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	8	3	contain	contain	1310:1316	arg1	N-terminus					1335:1344	their extreme N-terminus	1321:1344	their extreme N-terminus	1321:1344	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	3	contain	contain	1310:1316	arg2	medium					1276:1281	the medium and long ColXVIII isoforms	1272:1308	medium	1276:1281	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	3	contain	contain	1310:1316	arg2	isoforms					1301:1308	the medium and long ColXVIII isoforms	1272:1308	isoforms	1301:1308	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	3	contain	contain	1310:1316	arg1	isoforms					1301:1308	the medium and long ColXVIII isoforms	1272:1308	isoforms	1301:1308	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	3	contain	contain	1310:1316	arg1	medium					1276:1281	the medium and long ColXVIII isoforms	1272:1308	medium	1276:1281	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	3	contain	contain	1310:1316	arg2	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	2	4	theme	unknown	317:323	arg1	function					325:332	unknown function	317:332	unknown function	317:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	9	5	theme	interaction	1736:1746	arg1	hub					1748:1750	an interaction hub	1733:1750	an interaction hub affecting cell signaling	1733:1775	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	1	6	theme	short	226:230	arg1	isoforms					216:223	three isoforms	210:223	three isoforms: short, medium and long	210:247	Collagen XVIII (ColXVIII) is a non-fibrillar collagen and proteoglycan that exists in three isoforms: short, medium and long.					
30327321	7	7	theme	protein	1237:1243	arg1	half					1213:1216	half	1213:1216	half of the recombinant protein	1213:1243	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	9	8	theme	disordered	1540:1549	arg1	regions					1551:1557	intrinsically disordered regions	1526:1557	intrinsically disordered regions	1526:1557	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	7	9	theme	Thr	1160:1162	arg1	residues					1164:1171	numerous Ser and Thr residues	1143:1171	numerous Ser and Thr residues	1143:1171	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	4	10	gly	glycosylated	712:723	arg1	DUF959					725:730	glycosylated DUF959	712:730	glycosylated DUF959 in solution	712:742	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	6	11	theme	considerable	902:913	arg1	amounts					915:921	considerable amounts	902:921	considerable amounts of O-glycosylation	902:940	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	6	11	theme	considerable	902:913	arg1	O-glycosylation					926:940	O-glycosylation	926:940	O-glycosylation	926:940	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	7	12	theme	Ser	1152:1154	arg1	residues					1164:1171	numerous Ser and Thr residues	1143:1171	numerous Ser and Thr residues	1143:1171	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	2	13	theme	medium	254:259	arg1	isoforms					270:277	The medium and long isoforms	250:277	isoforms	270:277	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	1	14	theme	medium	233:238	arg1	isoforms					216:223	three isoforms	210:223	three isoforms: short, medium and long	210:247	Collagen XVIII (ColXVIII) is a non-fibrillar collagen and proteoglycan that exists in three isoforms: short, medium and long.					
30327321	7	15	theme	recombinant	1225:1235	arg1	protein					1237:1243	the recombinant protein	1221:1243	the recombinant protein	1221:1243	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	4	16	theme	light	663:667	arg1	scattering					669:678	static light scattering	656:678	static light scattering	656:678	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	3	17	theme	%	528:528	arg1	glycans					530:536	∼50% glycans	525:536	∼50% glycans	525:536	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	5	18	theme	maximum	838:844	arg1	dimension					846:854	a maximum dimension	836:854	a maximum dimension of ∼23 nm	836:864	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	4	19	theme	monomeric	692:700	arg1	state					702:706	a monomeric state	690:706	a monomeric state for glycosylated DUF959 in solution	690:742	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	6	20	theme	N-acetylgalactosamine-type	1065:1090	arg1	O-glycans					1092:1100	N-acetylgalactosamine-type O-glycans	1065:1100	N-acetylgalactosamine-type O-glycans	1065:1100	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	10	21	theme	cell-extracellular	1834:1851	arg1	interfaces					1860:1869	cell-extracellular matrix interfaces	1834:1869	cell-extracellular matrix interfaces	1834:1869	Moreover, the MUCL-C18 may also serve as a lubricant at cell-extracellular matrix interfaces.					
30327321	4	22	theme	DUF959	644:649	arg1	character					631:639	the disordered character	616:639	the disordered character of DUF959	616:649	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	6	23	theme	capable	990:996	arg1	SimpleCells					978:988	HEK293 SimpleCells	971:988	HEK293 SimpleCells capable of synthesizing only truncated O-glycans	971:1037	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	6	24	theme	Glycosidase	867:877	arg1	treatment					879:887	Glycosidase treatment	867:887	Glycosidase treatment	867:887	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	2	25	theme	disordered	446:455	arg1	DUF959					413:418	DUF959	413:418	DUF959	413:418	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	25	theme	disordered	446:455	arg1	domain					457:462	an intrinsically disordered domain	429:462	an intrinsically disordered domain	429:462	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	9	26	theme	flexible	1691:1698	arg1	ColXVIII					1721:1728	ColXVIII	1721:1728	ColXVIII	1721:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	9	26	theme	flexible	1691:1698	arg1	domain					1711:1716	the flexible mucin-like domain	1687:1716	the flexible mucin-like domain of ColXVIII	1687:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	0	27	theme	N-terminal	4:13	arg1	domain					15:20	The N-terminal domain	0:20	The N-terminal domain of unknown function (DUF959) in collagen XVIII	0:67	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	0	27	theme	N-terminal	4:13	arg1	function					33:40	unknown function	25:40	unknown function (DUF959)	25:49	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	6	28	theme	DUF959	961:966	arg1	expression					947:956	expression	947:956	expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans	947:1037	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	9	29	theme	mucin-like	1700:1709	arg1	ColXVIII					1721:1728	ColXVIII	1721:1728	ColXVIII	1721:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	9	29	theme	mucin-like	1700:1709	arg1	domain					1711:1716	the flexible mucin-like domain	1687:1716	the flexible mucin-like domain of ColXVIII	1687:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	9	30	theme	post-translational	1569:1586	arg1	modifications					1588:1600	their post-translational modifications	1563:1600	their post-translational modifications	1563:1600	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	8	31	theme	mucin-like	1396:1405	arg1	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	1	32	theme	non-fibrillar	155:167	arg1	collagen					169:176	a non-fibrillar collagen	153:176	a non-fibrillar collagen	153:176	Collagen XVIII (ColXVIII) is a non-fibrillar collagen and proteoglycan that exists in three isoforms: short, medium and long.					
30327321	1	32	theme	non-fibrillar	155:167	arg1	Collagen					124:131	Collagen XVIII	124:137	Collagen XVIII (ColXVIII)	124:148	Collagen XVIII (ColXVIII) is a non-fibrillar collagen and proteoglycan that exists in three isoforms: short, medium and long.					
30327321	1	33	theme	long	244:247	arg1	isoforms					216:223	three isoforms	210:223	three isoforms: short, medium and long	210:247	Collagen XVIII (ColXVIII) is a non-fibrillar collagen and proteoglycan that exists in three isoforms: short, medium and long.					
30327321	8	34	located	found	1426:1430	arg1	collagens					1441:1449	other collagens	1435:1449	other collagens	1435:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	34	located	found	1426:1430	arg2	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	4	35	from	DUF959	725:730	arg1	solution					735:742	solution	735:742	solution	735:742	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	4	36	theme	glycosylated	712:723	arg1	DUF959					725:730	glycosylated DUF959	712:730	glycosylated DUF959 in solution	712:742	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	5	37	theme	∼23 nm	859:864	arg1	dimension					846:854	a maximum dimension	836:854	a maximum dimension of ∼23 nm	836:864	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	5	38	theme	extended	803:810	arg1	molecule					822:829	a highly extended, flexible molecule	794:829	a highly extended, flexible molecule with a maximum dimension of ∼23 nm	794:864	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	6	39	theme	HEK293	971:976	arg1	SimpleCells					978:988	HEK293 SimpleCells	971:988	HEK293 SimpleCells capable of synthesizing only truncated O-glycans	971:1037	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	7	40	theme	DUF959	1107:1112	arg1	sequence					1114:1121	The DUF959 sequence	1103:1121	The DUF959 sequence	1103:1121	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	2	41	theme	long	265:268	arg1	isoforms					270:277	The medium and long isoforms	250:277	isoforms	270:277	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	6	42	theme	O-glycans	1092:1100	arg1	presence					1053:1060	the presence	1049:1060	the presence of N-acetylgalactosamine-type O-glycans	1049:1100	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	8	43	theme	O-glycosylated	1381:1394	arg1	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	9	44	theme	ColXVIII	1721:1728	arg1	ColXVIII					1721:1728	ColXVIII	1721:1728	ColXVIII	1721:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	9	44	theme	ColXVIII	1721:1728	arg1	domain					1711:1716	the flexible mucin-like domain	1687:1716	the flexible mucin-like domain of ColXVIII	1687:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	10	45	from	interfaces	1860:1869	arg1	MUCL-C18					1792:1799	the MUCL-C18	1788:1799	the MUCL-C18	1788:1799	Moreover, the MUCL-C18 may also serve as a lubricant at cell-extracellular matrix interfaces.					
30327321	10	45	from	interfaces	1860:1869	arg1	lubricant					1821:1829	lubricant	1821:1829	lubricant	1821:1829	Moreover, the MUCL-C18 may also serve as a lubricant at cell-extracellular matrix interfaces.					
30327321	2	46	theme	structure	376:384	arg1	predictions					386:396	our sequence-based secondary structure predictions	347:396	our sequence-based secondary structure predictions	347:396	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	0	47	theme	function	33:40	arg1	domain					15:20	The N-terminal domain	0:20	The N-terminal domain of unknown function (DUF959) in collagen XVIII	0:67	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	0	47	theme	function	33:40	arg1	function					33:40	unknown function	25:40	unknown function (DUF959)	25:49	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	3	48	contain	had	542:544	arg1	DUF959					477:482	Recombinant DUF959	465:482	Recombinant DUF959 produced in mammalian cells	465:510	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	3	48	contain	had	542:544	arg2	mass					558:561	a molecular mass	546:561	a molecular mass of 63 kDa	546:571	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	9	49	theme	domain	1711:1716	arg1	role					1679:1682	a role	1677:1682	a role of the flexible mucin-like domain of ColXVIII	1677:1728	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	8	50	theme	ColXVIII	1292:1299	arg1	isoforms					1301:1308	the medium and long ColXVIII isoforms	1272:1308	isoforms	1301:1308	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	2	51	theme	secondary	366:374	arg1	predictions					386:396	our sequence-based secondary structure predictions	347:396	our sequence-based secondary structure predictions	347:396	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	0	52	theme	unknown	25:31	arg1	function					33:40	unknown function	25:40	unknown function (DUF959)	25:49	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	0	52	theme	unknown	25:31	arg1	DUF959					43:48	DUF959	43:48	DUF959	43:48	The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated.					
30327321	8	53	gly	O-glycosylated	1381:1394	arg1	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	5	54	with	molecule	822:829	arg1	dimension					846:854	a maximum dimension	836:854	a maximum dimension of ∼23 nm	836:864	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	8	55	theme	extreme	1327:1333	arg1	N-terminus					1335:1344	their extreme N-terminus	1321:1344	their extreme N-terminus	1321:1344	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	3	56	theme	63 kDa	566:571	arg1	mass					558:561	a molecular mass	546:561	a molecular mass of 63 kDa	546:571	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	2	57	theme	sequence-based	351:364	arg1	predictions					386:396	our sequence-based secondary structure predictions	347:396	our sequence-based secondary structure predictions	347:396	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	9	58	theme	cell	1762:1765	arg1	signaling					1767:1775	cell signaling	1762:1775	cell signaling	1762:1775	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	8	59	dep	it	1474:1475	arg1	domain					1492:1497	the Mucin-like domain	1477:1497	it the Mucin-like domain	1474:1497	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	60	theme	elongated	1360:1368	arg1	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	3	61	theme	molecular	548:556	arg1	mass					558:561	a molecular mass	546:561	a molecular mass of 63 kDa	546:571	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	10	62	theme	matrix	1853:1858	arg1	interfaces					1860:1869	cell-extracellular matrix interfaces	1834:1869	cell-extracellular matrix interfaces	1834:1869	Moreover, the MUCL-C18 may also serve as a lubricant at cell-extracellular matrix interfaces.					
30327321	3	63	theme	Recombinant	465:475	arg1	DUF959					477:482	Recombinant DUF959	465:482	Recombinant DUF959 produced in mammalian cells	465:510	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	8	64	theme	Mucin-like	1481:1490	arg1	domain					1492:1497	the Mucin-like domain	1477:1497	it the Mucin-like domain	1474:1497	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	7	65	theme	numerous	1143:1150	arg1	residues					1164:1171	numerous Ser and Thr residues	1143:1171	numerous Ser and Thr residues	1143:1171	The DUF959 sequence is characterized by numerous Ser and Thr residues, and this accounts for the finding that half of the recombinant protein consists of glycans.					
30327321	2	66	theme	N-terminal	296:305	arg1	DUF959					335:340	DUF959	335:340	DUF959	335:340	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	66	theme	N-terminal	296:305	arg1	domain					307:312	a unique N-terminal domain	287:312	a unique N-terminal domain of unknown function	287:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	66	theme	N-terminal	296:305	arg1	function					325:332	unknown function	317:332	unknown function	317:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	67	contain	contain	279:285	arg2	domain					307:312	a unique N-terminal domain	287:312	a unique N-terminal domain of unknown function	287:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	67	contain	contain	279:285	arg2	function					325:332	unknown function	317:332	unknown function	317:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	67	contain	contain	279:285	arg1	isoforms					270:277	The medium and long isoforms	250:277	isoforms	270:277	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	67	contain	contain	279:285	arg2	DUF959					335:340	DUF959	335:340	DUF959	335:340	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	9	68	theme	protein	1624:1630	arg1	interactions					1632:1643	protein interactions	1624:1643	protein interactions	1624:1643	As intrinsically disordered regions and their post-translational modifications are often involved in protein interactions, our findings may point towards a role of the flexible mucin-like domain of ColXVIII as an interaction hub affecting cell signaling.					
30327321	2	69	theme	unique	289:294	arg1	DUF959					335:340	DUF959	335:340	DUF959	335:340	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	69	theme	unique	289:294	arg1	domain					307:312	a unique N-terminal domain	287:312	a unique N-terminal domain of unknown function	287:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	69	theme	unique	289:294	arg1	function					325:332	unknown function	317:332	unknown function	317:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	4	70	theme	Circular	574:581	arg1	spectroscopy					593:604	Circular dichroism spectroscopy	574:604	Circular dichroism spectroscopy	574:604	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	6	71	from	expression	947:956	arg1	SimpleCells					978:988	HEK293 SimpleCells	971:988	HEK293 SimpleCells capable of synthesizing only truncated O-glycans	971:1037	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	3	72	theme	∼50	525:527	arg1	%					528:528	%	528:528	%	528:528	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	4	73	theme	static	656:661	arg1	scattering					669:678	static light scattering	656:678	static light scattering	656:678	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	8	74	theme	disordered	1348:1357	arg1	domain					1407:1412	a disordered, elongated and highly O-glycosylated mucin-like domain	1346:1412	a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens	1346:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	8	75	theme	other	1435:1439	arg1	collagens					1441:1449	other collagens	1435:1449	other collagens	1435:1449	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	4	76	theme	disordered	620:629	arg1	character					631:639	the disordered character	616:639	the disordered character of DUF959	616:649	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30327321	8	77	theme	long	1287:1290	arg1	isoforms					1301:1308	the medium and long ColXVIII isoforms	1272:1308	isoforms	1301:1308	Thus, the medium and long ColXVIII isoforms contain at their extreme N-terminus a disordered, elongated and highly O-glycosylated mucin-like domain that is not found in other collagens, and we suggest naming it the Mucin-like domain in ColXVIII (MUCL-C18).					
30327321	3	78	theme	mammalian	496:504	arg1	cells					506:510	mammalian cells	496:510	mammalian cells	496:510	Recombinant DUF959 produced in mammalian cells consisted of ∼50% glycans and had a molecular mass of 63 kDa.					
30327321	5	79	theme	Small-angle	745:755	arg1	scattering					763:772	Small-angle X-ray scattering	745:772	Small-angle X-ray scattering	745:772	Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm.					
30327321	6	80	theme	O-glycosylation	926:940	arg1	amounts					915:921	considerable amounts	902:921	considerable amounts of O-glycosylation	902:940	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	6	80	theme	O-glycosylation	926:940	arg1	O-glycosylation					926:940	O-glycosylation	926:940	O-glycosylation	926:940	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	6	81	theme	truncated	1019:1027	arg1	O-glycans					1029:1037	only truncated O-glycans	1014:1037	only truncated O-glycans	1014:1037	Glycosidase treatment demonstrated considerable amounts of O-glycosylation, and expression of DUF959 in HEK293 SimpleCells capable of synthesizing only truncated O-glycans confirmed the presence of N-acetylgalactosamine-type O-glycans.					
30327321	2	82	theme	function	325:332	arg1	DUF959					335:340	DUF959	335:340	DUF959	335:340	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	82	theme	function	325:332	arg1	domain					307:312	a unique N-terminal domain	287:312	a unique N-terminal domain of unknown function	287:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	2	82	theme	function	325:332	arg1	function					325:332	unknown function	317:332	unknown function	317:332	The medium and long isoforms contain a unique N-terminal domain of unknown function, DUF959, and our sequence-based secondary structure predictions indicated that DUF959 could be an intrinsically disordered domain.					
30327321	4	83	theme	dichroism	583:591	arg1	spectroscopy					593:604	Circular dichroism spectroscopy	574:604	Circular dichroism spectroscopy	574:604	Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution.					
30973186	4	0	theme	Click	519:523	arg1	conjugation					525:535	Click conjugation	519:535	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides	519:588	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	2	1	attach	attach	242:247	arg2	We					222:223	We	222:223	We	222:223	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	2	1	attach	attach	242:247	arg1	EPO					270:272	EPO	270:272	EPO	270:272	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	1	2	link	N-linked	204:211	arg1	glycans					213:219	its N-linked glycans	200:219	its N-linked glycans	200:219	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	2	3	theme	alkyne	305:310	arg1	cycloaddition					312:324	copper-catalyzed azide alkyne cycloaddition	282:324	copper-catalyzed azide alkyne cycloaddition	282:324	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	4	4	with	conjugation	525:535	arg1	azides					583:588	biantennary N-glycan azides	562:588	biantennary N-glycan azides	562:588	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	4	5	theme	active	612:617	arg1	glycoconjugates					650:664	biologically active site-specifically modified EPO glycoconjugates	599:664	biologically active site-specifically modified EPO glycoconjugates	599:664	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	2	6	theme	azide	299:303	arg1	cycloaddition					312:324	copper-catalyzed azide alkyne cycloaddition	282:324	copper-catalyzed azide alkyne cycloaddition	282:324	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	3	7	theme	amino	375:379	arg1	acid					381:384	an alkyne-bearing non-natural amino acid	345:384	an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	345:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	7	theme	amino	375:379	arg1	Plk					387:389	Plk	387:389	Plk	387:389	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	8	theme	protein	483:489	arg1	purification					491:502	protein purification	483:502	protein purification	483:502	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	9	gly	N-glycosylation	399:413	arg2	38					425:426	38	425:426	38	425:426	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	9	gly	N-glycosylation	399:413	arg2	sites					415:419	the N-glycosylation sites 24, 38, and 83	395:434	the N-glycosylation sites 24, 38, and 83	395:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	9	gly	N-glycosylation	399:413	arg2	83					433:434	83	433:434	83	433:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	1	10	theme	biological	91:100	arg1	dependent					163:171	dependent	163:171	dependent	163:171	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	10	theme	biological	91:100	arg1	activity					102:109	The biological activity	87:109	The biological activity of the glycoprotein hormone erythropoietin (EPO)	87:158	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	11	gly	glycoprotein	118:129	arg1	glycoprotein					118:129	the glycoprotein hormone erythropoietin	114:152	the glycoprotein hormone erythropoietin (EPO)	114:158	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	3	12	theme	N-glycosylation	399:413	arg1	sites					415:419	the N-glycosylation sites 24, 38, and 83	395:434	the N-glycosylation sites 24, 38, and 83	395:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	13	with	variants	331:338	arg1	acid					381:384	an alkyne-bearing non-natural amino acid	345:384	an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	345:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	13	with	variants	331:338	arg1	Plk					387:389	Plk	387:389	Plk	387:389	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	4	14	theme	EPOs	552:555	arg1	conjugation					525:535	Click conjugation	519:535	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides	519:588	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	1	15	theme	glycoprotein	118:129	arg1	EPO					155:157	EPO	155:157	EPO	155:157	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	15	theme	glycoprotein	118:129	arg1	erythropoietin					139:152	the glycoprotein hormone erythropoietin	114:152	the glycoprotein hormone erythropoietin (EPO)	114:158	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	3	16	theme	amber	453:457	arg1	suppression					459:469	amber suppression	453:469	amber suppression followed by protein purification and refolding	453:516	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	0	17	theme	Erythropoietins	13:27	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Erythropoietins	0:27	Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans.					
30973186	4	18	theme	N-glycan	574:581	arg1	azides					583:588	biantennary N-glycan azides	562:588	biantennary N-glycan azides	562:588	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	2	19	theme	copper-catalyzed	282:297	arg1	cycloaddition					312:324	copper-catalyzed azide alkyne cycloaddition	282:324	copper-catalyzed azide alkyne cycloaddition	282:324	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	1	20	theme	hormone	131:137	arg1	EPO					155:157	EPO	155:157	EPO	155:157	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	20	theme	hormone	131:137	arg1	erythropoietin					139:152	the glycoprotein hormone erythropoietin	114:152	the glycoprotein hormone erythropoietin (EPO)	114:158	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	21	theme	glycans	213:219	arg1	structure					187:195	the structure	183:195	the structure of its N-linked glycans	183:219	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	4	22	theme	biantennary	562:572	arg1	azides					583:588	biantennary N-glycan azides	562:588	biantennary N-glycan azides	562:588	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	3	23	theme	EPO	327:329	arg1	variants					331:338	EPO variants	327:338	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	327:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	1	24	theme	erythropoietin	139:152	arg1	dependent					163:171	dependent	163:171	dependent	163:171	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	1	24	theme	erythropoietin	139:152	arg1	activity					102:109	The biological activity	87:109	The biological activity of the glycoprotein hormone erythropoietin (EPO)	87:158	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	0	25	theme	Complex-Type	63:74	arg1	N-Glycans					76:84	Complex-Type N-Glycans	63:84	Complex-Type N-Glycans	63:84	Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans.					
30973186	4	26	theme	alkynyl	544:550	arg1	EPOs					552:555	the alkynyl EPOs	540:555	the alkynyl EPOs	540:555	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	4	27	theme	modified	637:644	arg1	glycoconjugates					650:664	biologically active site-specifically modified EPO glycoconjugates	599:664	biologically active site-specifically modified EPO glycoconjugates	599:664	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30973186	3	28	theme	alkyne-bearing	348:361	arg1	acid					381:384	an alkyne-bearing non-natural amino acid	345:384	an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	345:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	28	theme	alkyne-bearing	348:361	arg1	Plk					387:389	Plk	387:389	Plk	387:389	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	1	29	theme	N-linked	204:211	arg1	glycans					213:219	its N-linked glycans	200:219	its N-linked glycans	200:219	The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans.					
30973186	3	30	theme	non-natural	363:373	arg1	acid					381:384	an alkyne-bearing non-natural amino acid	345:384	an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	345:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	30	theme	non-natural	363:373	arg1	Plk					387:389	Plk	387:389	Plk	387:389	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	31	from	sites	415:419	arg1	acid					381:384	an alkyne-bearing non-natural amino acid	345:384	an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83	345:434	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	3	31	from	sites	415:419	arg1	Plk					387:389	Plk	387:389	Plk	387:389	EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding.					
30973186	2	32	theme	defined	249:255	arg1	N-glycans					257:265	defined N-glycans	249:265	defined N-glycans	249:265	We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition.					
30973186	4	33	theme	EPO	646:648	arg1	glycoconjugates					650:664	biologically active site-specifically modified EPO glycoconjugates	599:664	biologically active site-specifically modified EPO glycoconjugates	599:664	Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.					
30674035	6	0	theme	IgG	911:913	arg1	profile					892:898	The N-glycosylation profile	872:898	The N-glycosylation profile of ALS CSF IgG	872:913	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	7	1	theme	galactosylated	1226:1239	arg1	structures					1241:1250	galactosylated structures	1226:1250	galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1226:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	3	2	with	comparison	477:486	arg1	diseases					531:538	other neurological diseases	512:538	other neurological diseases	512:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	3	2	with	comparison	477:486	arg1	group					503:507	a control group	493:507	a control group of other neurological diseases	493:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	6	3	theme	ALS	903:905	arg1	IgG					911:913	ALS CSF IgG	903:913	ALS CSF IgG	903:913	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	8	4	used	used	1589:1592	arg2	chain					1547:1551	phosphoneurofilament heavy chain	1520:1551	phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011)	1520:1576	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	4	used	used	1589:1592	arg2	marker					1607:1612	benchmark marker	1597:1612	benchmark marker for our group of patients	1597:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	3	5	theme	other	512:516	arg1	diseases					531:538	other neurological diseases	512:538	other neurological diseases	512:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	2	6	theme	blood	270:274	arg1	N-glycosylation					280:294	blood IgG N-glycosylation	270:294	blood IgG N-glycosylation	270:294	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	5	7	theme	mass	853:856	arg1	spectrometry					858:869	MALDI-TOF mass spectrometry	843:869	MALDI-TOF mass spectrometry	843:869	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	3	8	theme	diseases	531:538	arg1	diseases					531:538	other neurological diseases	512:538	other neurological diseases	512:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	3	8	theme	diseases	531:538	arg1	group					503:507	a control group	493:507	a control group of other neurological diseases	493:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	5	9	theme	exoglycosidase	814:827	arg1	digestion					829:837	exoglycosidase digestion	814:837	exoglycosidase digestion	814:837	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	4	10	theme	other	611:615	arg1	diseases					630:637	other neurological diseases	611:637	other neurological diseases (n = 10)	611:646	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	4	10	theme	other	611:615	arg1	n = 10					640:645	n = 10	640:645	n = 10	640:645	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	5	11	theme	purified	682:689	arg1	IgG					691:693	CSF purified IgG	678:693	CSF purified IgG	678:693	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	8	12	dep	chain	1547:1551	arg1	p = 0.011					1567:1575	p = 0.011	1567:1575	p = 0.011	1567:1575	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	12	dep	chain	1547:1551	arg1	AUC = 0.777					1554:1564	AUC = 0.777	1554:1564	AUC = 0.777	1554:1564	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	1	13	contain	have	237:240	arg1	neurofilaments					221:234	neurofilaments	221:234	neurofilaments	221:234	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	13	contain	have	237:240	arg1	biomarkers					209:218	the existing candidate biomarkers	186:218	the existing candidate biomarkers (neurofilaments)	186:235	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	13	contain	have	237:240	arg2	specificity					246:256	low specificity	242:256	low specificity	242:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	8	14	theme	ROC	1426:1428	arg1	analysis					1430:1437	ROC analysis	1426:1437	ROC analysis	1426:1437	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	2	15	dep	observed	306:313	arg1	whereas					351:357	whereas	351:357	whereas	351:357	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	4	16	with	patients	580:587	arg1	diseases					630:637	other neurological diseases	611:637	other neurological diseases (n = 10)	611:646	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	4	16	with	patients	580:587	arg1	n = 26					599:604	n = 26	599:604	n = 26	599:604	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	4	16	with	patients	580:587	arg1	ALS					594:596	ALS	594:596	ALS (n = 26)	594:605	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	4	16	with	patients	580:587	arg1	n = 10					640:645	n = 10	640:645	n = 10	640:645	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	6	17	theme	mono-	1023:1027	arg1	structures					1078:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	6	18	gly	N-glycosylation	876:890	arg1	IgG					911:913	ALS CSF IgG	903:913	ALS CSF IgG	903:913	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	1	19	theme	fatal	149:153	arg1	disease					168:174	a fatal motor neuron disease	147:174	a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity	147:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	19	theme	fatal	149:153	arg1	sclerosis					128:136	Amyotrophic lateral sclerosis	108:136	Amyotrophic lateral sclerosis (ALS)	108:142	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	6	20	theme	agalacto-	1012:1020	arg1	structures					1078:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	8	21	theme	phosphoneurofilament	1520:1539	arg1	chain					1547:1551	phosphoneurofilament heavy chain	1520:1551	phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011)	1520:1576	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	21	theme	phosphoneurofilament	1520:1539	arg1	marker					1607:1612	benchmark marker	1597:1612	benchmark marker for our group of patients	1597:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	0	22	theme	Lateral	89:95	arg1	Sclerosis					97:105	Amyotrophic Lateral Sclerosis	77:105	Amyotrophic Lateral Sclerosis	77:105	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	1	23	theme	neuron	161:166	arg1	disease					168:174	a fatal motor neuron disease	147:174	a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity	147:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	23	theme	neuron	161:166	arg1	sclerosis					128:136	Amyotrophic lateral sclerosis	108:136	Amyotrophic lateral sclerosis (ALS)	108:142	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	9	24	theme	N-glycan	1711:1718	arg1	galactosylation					1720:1734	IgG N-glycan galactosylation	1707:1734	IgG N-glycan galactosylation	1707:1734	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	5	25	attach	released	664:671	arg1	IgG					691:693	CSF purified IgG	678:693	CSF purified IgG	678:693	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	5	25	attach	released	664:671	arg2	N-Glycans					649:657	N-Glycans	649:657	N-Glycans	649:657	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	7	26	theme	ALS	1198:1200	arg1	IgG					1206:1208	ALS CSF IgG	1198:1208	ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1198:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	7	27	dep	median	1279:1284	arg1	p = 0.006					1339:1347	p = 0.006	1339:1347	p = 0.006	1339:1347	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	7	28	from	increase	1186:1193	arg1	IgG					1206:1208	ALS CSF IgG	1198:1208	ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1198:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	0	29	theme	Amyotrophic	77:87	arg1	Sclerosis					97:105	Amyotrophic Lateral Sclerosis	77:105	Amyotrophic Lateral Sclerosis	77:105	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	1	30	theme	Amyotrophic	108:118	arg1	disease					168:174	a fatal motor neuron disease	147:174	a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity	147:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	30	theme	Amyotrophic	108:118	arg1	ALS					139:141	ALS	139:141	ALS	139:141	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	30	theme	Amyotrophic	108:118	arg1	sclerosis					128:136	Amyotrophic lateral sclerosis	108:136	Amyotrophic lateral sclerosis (ALS)	108:142	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	8	31	theme	predictive	1355:1364	arg1	value					1366:1370	The predictive value	1351:1370	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis	1351:1437	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	31	theme	predictive	1355:1364	arg1	comparable					1498:1507	comparable	1498:1507	comparable	1498:1507	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	0	32	theme	Fluid	24:28	arg1	N-Glycosylation					34:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	3	33	theme	control	495:501	arg1	diseases					531:538	other neurological diseases	512:538	other neurological diseases	512:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	3	33	theme	control	495:501	arg1	group					503:507	a control group	493:507	a control group of other neurological diseases	493:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	9	34	theme	larger	1766:1771	arg1	cohorts					1773:1779	larger cohorts	1766:1779	larger cohorts of patients and controls	1766:1804	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	2	35	located	observed	306:313	arg1	diseases					326:333	several diseases	318:333	several diseases	318:333	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	2	35	located	observed	306:313	arg2	Changes					259:265	Changes	259:265	Changes in blood IgG N-glycosylation	259:294	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	2	35	located	observed	306:313	arg1	ALS					346:348	ALS	346:348	ALS	346:348	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	8	36	theme	heavy	1541:1545	arg1	chain					1547:1551	phosphoneurofilament heavy chain	1520:1551	phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011)	1520:1576	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	36	theme	heavy	1541:1545	arg1	marker					1607:1612	benchmark marker	1597:1612	benchmark marker for our group of patients	1597:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	37	theme	Gal-index	1379:1387	arg1	value					1366:1370	The predictive value	1351:1370	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis	1351:1437	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	37	theme	Gal-index	1379:1387	arg1	comparable					1498:1507	comparable	1498:1507	comparable	1498:1507	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	3	38	theme	ALS	461:463	arg1	patients					465:472	ALS patients	461:472	ALS patients	461:472	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	0	39	theme	Potential	53:61	arg1	Biomarker					63:71	Potential Biomarker	53:71	Potential Biomarker for Amyotrophic Lateral Sclerosis	53:105	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	3	40	theme	IgG	452:454	arg1	N-glycans					435:443	N-glycans	435:443	N-glycans of CSF IgG	435:454	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	8	41	theme	benchmark	1597:1605	arg1	chain					1547:1551	phosphoneurofilament heavy chain	1520:1551	phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011)	1520:1576	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	41	theme	benchmark	1597:1605	arg1	marker					1607:1612	benchmark marker	1597:1612	benchmark marker for our group of patients	1597:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	42	contain	had	1439:1441	arg1	value					1366:1370	The predictive value	1351:1370	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis	1351:1437	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	42	contain	had	1439:1441	arg2	utility					1453:1459	potential utility	1443:1459	potential utility	1443:1459	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	42	contain	had	1439:1441	arg1	comparable					1498:1507	comparable	1498:1507	comparable	1498:1507	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	42	contain	had	1439:1441	arg2	test					1477:1480	a diagnostic test	1464:1480	a diagnostic test for ALS	1464:1488	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	2	43	theme	fluid	373:377	arg1	IgG					385:387	cerebrospinal fluid (CSF) IgG	359:387	cerebrospinal fluid (CSF) IgG	359:387	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	7	44	theme	control	1132:1138	arg1	patients					1140:1147	ALS and control patients	1124:1147	patients	1140:1147	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	6	45	theme	CSF	907:909	arg1	IgG					911:913	ALS CSF IgG	903:913	ALS CSF IgG	903:913	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	7	46	theme	structures	1241:1250	arg1	level					1217:1221	the level	1213:1221	the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1213:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	7	47	theme	ALS	1124:1126	arg1	patients					1140:1147	ALS and control patients	1124:1147	patients	1140:1147	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	8	48	theme	patients	1631:1638	arg1	patients					1631:1638	patients	1631:1638	patients	1631:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	48	theme	patients	1631:1638	arg1	group					1622:1626	our group	1618:1626	our group of patients	1618:1638	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	2	49	theme	IgG	276:278	arg1	N-glycosylation					280:294	blood IgG N-glycosylation	270:294	blood IgG N-glycosylation	270:294	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	5	50	theme	MALDI-TOF	843:851	arg1	spectrometry					858:869	MALDI-TOF mass spectrometry	843:869	MALDI-TOF mass spectrometry	843:869	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	6	51	theme	N-glycosylation	876:890	arg1	profile					892:898	The N-glycosylation profile	872:898	The N-glycosylation profile of ALS CSF IgG	872:913	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	4	52	theme	neurological	617:628	arg1	diseases					630:637	other neurological diseases	611:637	other neurological diseases (n = 10)	611:646	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	4	52	theme	neurological	617:628	arg1	n = 10					640:645	n = 10	640:645	n = 10	640:645	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	2	53	theme	several	318:324	arg1	diseases					326:333	several diseases	318:333	several diseases	318:333	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	2	53	theme	several	318:324	arg1	ALS					346:348	ALS	346:348	ALS	346:348	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	5	54	with	combination	797:807	arg1	digestion					829:837	exoglycosidase digestion	814:837	exoglycosidase digestion	814:837	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	5	54	with	combination	797:807	arg1	spectrometry					858:869	MALDI-TOF mass spectrometry	843:869	MALDI-TOF mass spectrometry	843:869	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	3	55	theme	neurological	518:529	arg1	diseases					531:538	other neurological diseases	512:538	other neurological diseases	512:538	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	1	56	theme	existing	190:197	arg1	neurofilaments					221:234	neurofilaments	221:234	neurofilaments	221:234	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	56	theme	existing	190:197	arg1	biomarkers					209:218	the existing candidate biomarkers	186:218	the existing candidate biomarkers (neurofilaments)	186:235	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	6	57	theme	proximal	969:976	arg1	fucose					978:983	proximal fucose	969:983	proximal fucose	969:983	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	5	58	theme	CSF	678:680	arg1	IgG					691:693	CSF purified IgG	678:693	CSF purified IgG	678:693	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	6	59	theme	diantennary	928:938	arg1	N-glycans					940:948	diantennary N-glycans	928:948	diantennary N-glycans	928:948	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	8	60	theme	potential	1443:1451	arg1	test					1477:1480	a diagnostic test	1464:1480	a diagnostic test for ALS	1464:1488	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	60	theme	potential	1443:1451	arg1	utility					1453:1459	potential utility	1443:1459	potential utility	1443:1459	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	7	61	theme	level	1217:1221	arg1	IgG					1206:1208	ALS CSF IgG	1198:1208	ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1198:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	8	62	dep	Gal-index	1379:1387	arg1	AUC = 0.792					1390:1400	AUC = 0.792	1390:1400	AUC = 0.792	1390:1400	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	63	theme	diagnostic	1466:1475	arg1	test					1477:1480	a diagnostic test	1464:1480	a diagnostic test for ALS	1464:1488	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	8	63	theme	diagnostic	1466:1475	arg1	utility					1453:1459	potential utility	1443:1459	potential utility	1443:1459	The predictive value of the Gal-index (AUC = 0.792, p = 0.007) considering ROC analysis had potential utility as a diagnostic test for ALS and was comparable to that of phosphoneurofilament heavy chain (AUC = 0.777, p = 0.011), which was used as benchmark marker for our group of patients.					
30674035	1	64	theme	motor	155:159	arg1	disease					168:174	a fatal motor neuron disease	147:174	a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity	147:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	64	theme	motor	155:159	arg1	sclerosis					128:136	Amyotrophic lateral sclerosis	108:136	Amyotrophic lateral sclerosis (ALS)	108:142	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	9	65	theme	IgG	1707:1709	arg1	galactosylation					1720:1734	IgG N-glycan galactosylation	1707:1734	IgG N-glycan galactosylation	1707:1734	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	7	66	theme	CSF	1202:1204	arg1	IgG					1206:1208	ALS CSF IgG	1198:1208	ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006)	1198:1348	Differences between ALS and control patients were observed; most relevant was the increase in ALS CSF IgG of the level of galactosylated structures defined here as Gal-index (median 46.87 and 40.50% for ALS and controls, respectively; p = 0.006).					
30674035	6	67	theme	bisecting	994:1002	arg1	GlcNAc					1004:1009	some bisecting GlcNAc	989:1009	some bisecting GlcNAc	989:1009	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	9	68	theme	galactosylation	1720:1734	arg1	potential					1694:1702	the potential	1690:1702	the potential of IgG N-glycan galactosylation	1690:1734	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	5	69	theme	peptide	700:706	arg1	F					722:722	peptide N-glycosidase F	700:722	peptide N-glycosidase F	700:722	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	1	70	theme	candidate	199:207	arg1	neurofilaments					221:234	neurofilaments	221:234	neurofilaments	221:234	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	70	theme	candidate	199:207	arg1	biomarkers					209:218	the existing candidate biomarkers	186:218	the existing candidate biomarkers (neurofilaments)	186:235	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	5	71	theme	NP-HPLC	771:777	arg1	chromatography					779:792	NP-HPLC chromatography	771:792	NP-HPLC chromatography	771:792	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	6	72	theme	α2,6-sialylated	1062:1076	arg1	structures					1078:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	0	73	theme	IgG	30:32	arg1	N-Glycosylation					34:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	1	74	theme	lateral	120:126	arg1	disease					168:174	a fatal motor neuron disease	147:174	a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity	147:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	74	theme	lateral	120:126	arg1	ALS					139:141	ALS	139:141	ALS	139:141	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	1	74	theme	lateral	120:126	arg1	sclerosis					128:136	Amyotrophic lateral sclerosis	108:136	Amyotrophic lateral sclerosis (ALS)	108:142	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
30674035	4	75	theme	Cerebrospinal	541:553	arg1	fluid					555:559	Cerebrospinal fluid	541:559	Cerebrospinal fluid	541:559	Cerebrospinal fluid was collected from patients with ALS (n = 26) and other neurological diseases (n = 10).					
30674035	3	76	theme	CSF	448:450	arg1	IgG					452:454	CSF IgG	448:454	CSF IgG	448:454	Here, we characterized N-glycans of CSF IgG from ALS patients in comparison with a control group of other neurological diseases.					
30674035	2	77	from	Changes	259:265	arg1	N-glycosylation					280:294	blood IgG N-glycosylation	270:294	blood IgG N-glycosylation	270:294	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	6	78	theme	digalactosylated	1034:1049	arg1	structures					1078:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	5	79	theme	N-glycosidase	708:720	arg1	F					722:722	peptide N-glycosidase F	700:722	peptide N-glycosidase F	700:722	N-Glycans were released from CSF purified IgG with peptide N-glycosidase F, labeled with 2-aminobenzamide and analyzed by NP-HPLC chromatography in combination with exoglycosidase digestion and MALDI-TOF mass spectrometry.					
30674035	9	80	theme	patients	1784:1791	arg1	cohorts					1773:1779	larger cohorts	1766:1779	larger cohorts of patients and controls	1766:1804	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	9	81	theme	controls	1797:1804	arg1	cohorts					1773:1779	larger cohorts	1766:1779	larger cohorts of patients and controls	1766:1804	The results provide the basis to further explore the potential of IgG N-glycan galactosylation as biomarker for ALS by using larger cohorts of patients and controls.					
30674035	6	82	gly	α2,6-sialylated	1062:1076	arg1	structures					1078:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures	1012:1087	The N-glycosylation profile of ALS CSF IgG consisted of diantennary N-glycans predominantly with proximal fucose and some bisecting GlcNAc; agalacto-, mono-, and digalactosylated as well as α2,6-sialylated structures were detected.					
30674035	2	83	theme	cerebrospinal	359:371	arg1	CSF					380:382	CSF	380:382	CSF	380:382	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	2	83	theme	cerebrospinal	359:371	arg1	fluid					373:377	cerebrospinal fluid	359:377	cerebrospinal fluid (CSF) IgG	359:387	Changes in blood IgG N-glycosylation have been observed in several diseases, including ALS, whereas cerebrospinal fluid (CSF) IgG has been less studied.					
30674035	0	84	theme	Cerebrospinal	10:22	arg1	N-Glycosylation					34:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Cerebrospinal Fluid IgG N-Glycosylation	10:48	Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis.					
30674035	1	85	theme	low	242:244	arg1	specificity					246:256	low specificity	242:256	low specificity	242:256	Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease for which the existing candidate biomarkers (neurofilaments) have low specificity.					
31108197	6	0	theme	mannose	1145:1151	arg1	Man6GlcNAc2					1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	0	theme	mannose	1145:1151	arg1	structure					1162:1170	the main high mannose N-linked structure	1131:1170	the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing	1131:1332	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	10	1	theme	effective	1998:2006	arg1	regeneration					2008:2019	an effective regeneration	1995:2019	an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction	1995:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	6	2	theme	complex	1287:1293	arg1	structures					1295:1304	complex structures	1287:1304	complex structures	1287:1304	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	3	theme	glycans	1247:1253	arg1	expression					1213:1222	higher expression	1206:1222	higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures	1206:1304	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	2	4	theme	potential	301:309	arg1	regulators					274:283	key regulators	270:283	key regulators of regenerative potential	270:309	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	6	5	theme	sialylated	1227:1236	arg1	glycans					1247:1253	sialylated N-linked glycans	1227:1253	sialylated N-linked glycans	1227:1253	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	8	6	link	O-linked	1507:1514	arg1	glycans					1516:1522	O-linked glycans	1507:1522	O-linked glycans from mature tissue	1507:1541	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	10	7	theme	mammalian	2028:2036	arg1	heart					2038:2042	the mammalian heart	2024:2042	the mammalian heart	2024:2042	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	0	8	theme	Distinct	0:7	arg1	glycosylation					9:21	Distinct glycosylation	0:21	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue	0:89	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	5	9	link	N-linked	1046:1053	arg1	glycans					1055:1061	complex N-linked glycans	1038:1061	complex N-linked glycans	1038:1061	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	8	10	link	N-linked	1652:1659	arg1	glycans					1661:1667	all sialylated N-linked glycans	1637:1667	all sialylated N-linked glycans detected	1637:1676	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	9	11	theme	neonatal	1774:1781	arg1	structures					1783:1792	the specific neonatal structures	1761:1792	the specific neonatal structures found	1761:1798	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	2	12	theme	neonatal	247:254	arg1	tissue					256:261	cardiac neonatal tissue	239:261	cardiac neonatal tissue	239:261	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	5	13	theme	cardiac	852:858	arg1	glycosylation					860:872	neonatal cardiac glycosylation	843:872	neonatal cardiac glycosylation in the left ventricle	843:894	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	7	14	theme	core	1426:1429	arg1	structure					1447:1455	one sulfated core type 2 O-linked structure	1413:1455	one sulfated core type 2 O-linked structure	1413:1455	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	4	15	theme	left	735:738	arg1	ventricle					740:748	the right or left ventricle	722:748	the right or left ventricle using lectin histochemistry and microarray profiling	722:801	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	6	16	gly	sialylated	1227:1236	arg1	glycans					1247:1253	sialylated N-linked glycans	1227:1253	sialylated N-linked glycans	1227:1253	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	8	17	theme	mature	1529:1534	arg1	tissue					1536:1541	mature tissue	1529:1541	mature tissue	1529:1541	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	6	18	theme	fucosylation	1270:1281	arg1	expression					1213:1222	higher expression	1206:1222	higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures	1206:1304	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	8	19	gly	sialylated	1641:1650	arg1	glycans					1661:1667	all sialylated N-linked glycans	1637:1667	all sialylated N-linked glycans detected	1637:1676	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	2	20	theme	key	270:272	arg1	regulators					274:283	key regulators	270:283	key regulators of regenerative potential	270:309	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	5	21	theme	higher	969:974	arg1	expression					976:985	a higher expression	967:985	a higher expression of high mannose structures	967:1012	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	6	22	theme	lower	1259:1263	arg1	fucosylation					1270:1281	lower core fucosylation	1259:1281	lower core fucosylation	1259:1281	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	0	23	theme	adult	67:71	arg1	tissue					84:89	neonatal versus adult myocardial tissue	51:89	neonatal versus adult myocardial tissue	51:89	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	10	24	theme	New	1911:1913	arg1	strategies					1915:1924	New strategies	1911:1924	New strategies targeting tissue glycosylation	1911:1955	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	10	24	theme	New	1911:1913	arg1	contributor					1972:1982	a key contributor	1966:1982	a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction	1966:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	5	25	theme	mass	930:933	arg1	analysis					949:956	mass spectrometric analysis	930:956	mass spectrometric analysis	930:956	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	3	26	theme	extracellular	338:350	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	26	theme	extracellular	338:350	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	26	theme	extracellular	338:350	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	8	27	theme	N-acetylneuraminic	1558:1575	arg1	Neu5Ac					1583:1588	Neu5Ac	1583:1588	Neu5Ac	1583:1588	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	27	theme	N-acetylneuraminic	1558:1575	arg1	acid					1577:1580	N-acetylneuraminic acid	1558:1580	N-acetylneuraminic acid (Neu5Ac)	1558:1589	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	4	28	theme	right	726:730	arg1	ventricle					740:748	the right or left ventricle	722:748	the right or left ventricle using lectin histochemistry and microarray profiling	722:801	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	3	29	from	variation	443:451	arg1	glycosylation					464:476	cardiac glycosylation	456:476	cardiac glycosylation from neonatal tissue to maturation	456:511	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	29	from	variation	443:451	arg1	tissue					492:497	neonatal tissue	483:497	neonatal tissue	483:497	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	9	30	theme	intracellular	1732:1744	arg1	communication					1746:1758	intracellular communication	1732:1758	intracellular communication	1732:1758	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	9	31	theme	associated	1854:1863	arg1	capacity					1878:1885	the neonatal associated regenerative capacity	1841:1885	the neonatal associated regenerative capacity of the mammalian heart	1841:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	7	32	theme	sulfated	1417:1424	arg1	structure					1447:1455	one sulfated core type 2 O-linked structure	1413:1455	one sulfated core type 2 O-linked structure	1413:1455	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	1	33	theme	regenerative	114:125	arg1	potential					127:135	regenerative potential	114:135	regenerative potential restricted to early neonatal stage and lost within seven days after birth	114:209	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	1	34	theme	early	151:155	arg1	stage					166:170	early neonatal stage	151:170	early neonatal stage	151:170	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	3	35	theme	known	377:381	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	35	theme	known	377:381	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	35	theme	known	377:381	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	1	36	dep	potential	127:135	arg1	restricted					137:146	restricted	137:146	restricted	137:146	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	1	36	dep	potential	127:135	arg1	lost					176:179	lost	176:179	lost within seven days after birth	176:209	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	3	37	theme	function	417:424	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	37	theme	function	417:424	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	37	theme	function	417:424	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	8	38	theme	N-glycolylneuraminic	1595:1614	arg1	Neu5Gc					1622:1627	Neu5Gc	1622:1627	Neu5Gc	1622:1627	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	38	theme	N-glycolylneuraminic	1595:1614	arg1	acid					1616:1619	N-glycolylneuraminic acid	1595:1619	N-glycolylneuraminic acid (Neu5Gc)	1595:1628	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	3	39	theme	cardiac	456:462	arg1	glycosylation					464:476	cardiac glycosylation	456:476	cardiac glycosylation from neonatal tissue to maturation	456:511	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	5	40	theme	neonatal	843:850	arg1	glycosylation					860:872	neonatal cardiac glycosylation	843:872	neonatal cardiac glycosylation in the left ventricle	843:894	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	9	41	theme	heart	1904:1908	arg1	capacity					1878:1885	the neonatal associated regenerative capacity	1841:1885	the neonatal associated regenerative capacity of the mammalian heart	1841:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	4	42	theme	adult	578:582	arg1	ventricle					596:604	the adult rat cardiac ventricle	574:604	the adult rat cardiac ventricle	574:604	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	9	43	theme	regenerative	1865:1876	arg1	capacity					1878:1885	the neonatal associated regenerative capacity	1841:1885	the neonatal associated regenerative capacity of the mammalian heart	1841:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	4	44	theme	microarray	782:791	arg1	profiling					793:801	microarray profiling	782:801	microarray profiling	782:801	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	7	45	theme	neonatal	1475:1482	arg1	tissue					1484:1489	neonatal tissue	1475:1489	neonatal tissue	1475:1489	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	5	46	theme	mannose	995:1001	arg1	structures					1003:1012	high mannose structures	990:1012	high mannose structures	990:1012	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	7	47	theme	O-linked	1371:1378	arg1	glycans					1380:1386	mucin core type 2 O-linked glycans	1353:1386	mucin core type 2 O-linked glycans	1353:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	4	48	theme	cardiac	588:594	arg1	ventricle					596:604	the adult rat cardiac ventricle	574:604	the adult rat cardiac ventricle	574:604	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	0	49	gly	glycosylation	9:21	arg1	proteins					35:42	membrane proteins	26:42	membrane proteins	26:42	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	7	50	theme	type	1364:1367	arg1	glycans					1380:1386	mucin core type 2 O-linked glycans	1353:1386	mucin core type 2 O-linked glycans	1353:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	5	51	gly	glycosylation	860:872	arg1	ventricle					886:894	the left ventricle	877:894	the left ventricle	877:894	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	0	52	theme	neonatal	51:58	arg1	tissue					84:89	neonatal versus adult myocardial tissue	51:89	neonatal versus adult myocardial tissue	51:89	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	5	53	theme	neonatal	1084:1091	arg1	tissue					1093:1098	the three-day-old neonatal tissue	1066:1098	the three-day-old neonatal tissue	1066:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	7	54	theme	mucin	1353:1357	arg1	glycans					1380:1386	mucin core type 2 O-linked glycans	1353:1386	mucin core type 2 O-linked glycans	1353:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	8	55	contain	contained	1543:1551	arg1	glycans					1516:1522	O-linked glycans	1507:1522	O-linked glycans from mature tissue	1507:1541	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	55	contain	contained	1543:1551	arg2	acid					1616:1619	N-glycolylneuraminic acid	1595:1619	N-glycolylneuraminic acid (Neu5Gc)	1595:1628	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	55	contain	contained	1543:1551	arg2	Neu5Ac					1583:1588	Neu5Ac	1583:1588	Neu5Ac	1583:1588	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	55	contain	contained	1543:1551	arg2	Neu5Gc					1622:1627	Neu5Gc	1622:1627	Neu5Gc	1622:1627	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	55	contain	contained	1543:1551	arg2	acid					1577:1580	N-acetylneuraminic acid	1558:1580	N-acetylneuraminic acid (Neu5Ac)	1558:1589	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	56	theme	sialylated	1641:1650	arg1	glycans					1661:1667	all sialylated N-linked glycans	1637:1667	all sialylated N-linked glycans detected	1637:1676	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	5	57	theme	complex	1038:1044	arg1	glycans					1055:1061	complex N-linked glycans	1038:1061	complex N-linked glycans	1038:1061	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	6	58	link	N-linked	1153:1160	arg1	Man6GlcNAc2					1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	58	link	N-linked	1153:1160	arg1	structure					1162:1170	the main high mannose N-linked structure	1131:1170	the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing	1131:1332	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	5	59	theme	glycans	1055:1061	arg1	expression					976:985	a higher expression	967:985	a higher expression of high mannose structures	967:1012	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	5	59	theme	glycans	1055:1061	arg1	expression					1024:1033	lower expression	1018:1033	lower expression of complex N-linked glycans in the three-day-old neonatal tissue	1018:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	6	60	theme	N-linked	1153:1160	arg1	Man6GlcNAc2					1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	60	theme	N-linked	1153:1160	arg1	structure					1162:1170	the main high mannose N-linked structure	1131:1170	the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing	1131:1332	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	5	61	from	expression	1024:1033	arg1	tissue					1093:1098	the three-day-old neonatal tissue	1066:1098	the three-day-old neonatal tissue	1066:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	6	62	theme	high	1140:1143	arg1	Man6GlcNAc2					1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	62	theme	high	1140:1143	arg1	structure					1162:1170	the main high mannose N-linked structure	1131:1170	the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing	1131:1332	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	7	63	link	O-linked	1371:1378	arg1	glycans					1380:1386	mucin core type 2 O-linked glycans	1353:1386	mucin core type 2 O-linked glycans	1353:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	6	64	theme	N-linked	1238:1245	arg1	glycans					1247:1253	sialylated N-linked glycans	1227:1253	sialylated N-linked glycans	1227:1253	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	10	65	theme	heart	2038:2042	arg1	regeneration					2008:2019	an effective regeneration	1995:2019	an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction	1995:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	2	66	theme	regenerative	288:299	arg1	potential					301:309	regenerative potential	288:309	regenerative potential	288:309	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	9	67	theme	neonatal	1845:1852	arg1	capacity					1878:1885	the neonatal associated regenerative capacity	1841:1885	the neonatal associated regenerative capacity of the mammalian heart	1841:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	5	68	theme	adult	910:914	arg1	tissues					916:922	adult tissues	910:922	adult tissues using mass spectrometric analysis	910:956	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	9	69	theme	specific	1765:1772	arg1	structures					1783:1792	the specific neonatal structures	1761:1792	the specific neonatal structures found	1761:1798	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	6	70	theme	higher	1206:1211	arg1	expression					1213:1222	higher expression	1206:1222	higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures	1206:1304	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	10	71	from	regeneration	2008:2019	arg1	infarction					2089:2098	myocardial infarction	2078:2098	myocardial infarction	2078:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	10	71	from	regeneration	2008:2019	arg1	scenarios					2060:2068	pathological scenarios	2047:2068	pathological scenarios such as myocardial infarction	2047:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	8	72	theme	O-linked	1507:1514	arg1	glycans					1516:1522	O-linked glycans	1507:1522	O-linked glycans from mature tissue	1507:1541	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	10	73	theme	pathological	2047:2058	arg1	infarction					2089:2098	myocardial infarction	2078:2098	myocardial infarction	2078:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	10	73	theme	pathological	2047:2058	arg1	scenarios					2060:2068	pathological scenarios	2047:2068	pathological scenarios such as myocardial infarction	2047:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	5	74	theme	left	881:884	arg1	ventricle					886:894	the left ventricle	877:894	the left ventricle	877:894	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	7	75	theme	O-linked	1438:1445	arg1	structure					1447:1455	one sulfated core type 2 O-linked structure	1413:1455	one sulfated core type 2 O-linked structure	1413:1455	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	1	76	contain	have	109:112	arg1	hearts					102:107	Mammalian hearts	92:107	Mammalian hearts	92:107	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	1	76	contain	have	109:112	arg2	potential					127:135	regenerative potential	114:135	regenerative potential restricted to early neonatal stage and lost within seven days after birth	114:209	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	4	77	gly	glycosylation	705:717	arg1	ventricle					740:748	the right or left ventricle	722:748	the right or left ventricle using lectin histochemistry and microarray profiling	722:801	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	2	78	theme	cardiac	239:245	arg1	tissue					256:261	cardiac neonatal tissue	239:261	cardiac neonatal tissue	239:261	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	7	79	theme	type	1431:1434	arg1	structure					1447:1455	one sulfated core type 2 O-linked structure	1413:1455	one sulfated core type 2 O-linked structure	1413:1455	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	10	80	theme	myocardial	2078:2087	arg1	infarction					2089:2098	myocardial infarction	2078:2098	myocardial infarction	2078:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	10	81	theme	tissue	1936:1941	arg1	glycosylation					1943:1955	tissue glycosylation	1936:1955	tissue glycosylation	1936:1955	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	9	82	from	role	1815:1818	arg1	capacity					1878:1885	the neonatal associated regenerative capacity	1841:1885	the neonatal associated regenerative capacity of the mammalian heart	1841:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	3	83	theme	cell	321:324	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	83	theme	cell	321:324	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	83	theme	cell	321:324	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	6	84	theme	core	1265:1268	arg1	fucosylation					1270:1281	lower core fucosylation	1259:1281	lower core fucosylation	1259:1281	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	4	85	theme	ventricle	740:748	arg1	glycosylation					705:717	the glycosylation	701:717	the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling	701:801	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	0	86	theme	myocardial	73:82	arg1	tissue					84:89	neonatal versus adult myocardial tissue	51:89	neonatal versus adult myocardial tissue	51:89	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	4	87	gly	glycosylation	557:569	arg1	ventricle					596:604	the adult rat cardiac ventricle	574:604	the adult rat cardiac ventricle	574:604	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	1	88	theme	neonatal	157:164	arg1	stage					166:170	early neonatal stage	151:170	early neonatal stage	151:170	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	6	89	link	N-linked	1238:1245	arg1	glycans					1247:1253	sialylated N-linked glycans	1227:1253	sialylated N-linked glycans	1227:1253	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	5	90	theme	spectrometric	935:947	arg1	analysis					949:956	mass spectrometric analysis	930:956	mass spectrometric analysis	930:956	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	3	91	theme	matrix	352:357	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	91	theme	matrix	352:357	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	91	theme	matrix	352:357	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	0	92	from	glycosylation	9:21	arg1	proteins					35:42	membrane proteins	26:42	membrane proteins	26:42	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	5	93	theme	structures	1003:1012	arg1	expression					976:985	a higher expression	967:985	a higher expression of high mannose structures	967:1012	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	5	93	theme	structures	1003:1012	arg1	expression					1024:1033	lower expression	1018:1033	lower expression of complex N-linked glycans in the three-day-old neonatal tissue	1018:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	3	94	theme	tissue	397:402	arg1	glycosylation					359:371	extracellular matrix glycosylation	338:371	extracellular matrix glycosylation	338:371	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	94	theme	tissue	397:402	arg1	modulators					383:392	known modulators	377:392	known modulators of tissue and cellular function and development	377:440	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	94	theme	tissue	397:402	arg1	surface					326:332	cell surface	321:332	cell surface	321:332	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	8	95	from	tissue	1536:1541	arg1	glycans					1516:1522	O-linked glycans	1507:1522	O-linked glycans from mature tissue	1507:1541	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	5	96	from	glycosylation	860:872	arg1	ventricle					886:894	the left ventricle	877:894	the left ventricle	877:894	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	5	97	theme	lower	1018:1022	arg1	expression					1024:1033	lower expression	1018:1033	lower expression of complex N-linked glycans in the three-day-old neonatal tissue	1018:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	6	98	theme	main	1135:1138	arg1	Man6GlcNAc2					1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2	1101:1111	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	6	98	theme	main	1135:1138	arg1	structure					1162:1170	the main high mannose N-linked structure	1131:1170	the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing	1131:1332	Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing.					
31108197	8	99	contain	contained	1678:1686	arg1	glycans					1661:1667	all sialylated N-linked glycans	1637:1667	all sialylated N-linked glycans detected	1637:1676	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	8	99	contain	contained	1678:1686	arg2	Neu5Ac					1693:1698	Neu5Ac	1693:1698	Neu5Ac	1693:1698	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	3	100	theme	neonatal	483:490	arg1	tissue					492:497	neonatal tissue	483:497	neonatal tissue	483:497	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	1	101	theme	Mammalian	92:100	arg1	hearts					102:107	Mammalian hearts	92:107	Mammalian hearts	92:107	Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth.					
31108197	7	102	theme	glycans	1380:1386	arg1	occurrence					1339:1348	The occurrence	1335:1348	The occurrence of mucin core type 2 O-linked glycans	1335:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	9	103	theme	mammalian	1894:1902	arg1	heart					1904:1908	the mammalian heart	1890:1908	the mammalian heart	1890:1908	As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart.					
31108197	4	104	theme	rat	584:586	arg1	ventricle					596:604	the adult rat cardiac ventricle	574:604	the adult rat cardiac ventricle	574:604	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	10	105	theme	key	1968:1970	arg1	contributor					1972:1982	a key contributor	1966:1982	a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction	1966:2098	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	10	105	theme	key	1968:1970	arg1	strategies					1915:1924	New strategies	1911:1924	New strategies targeting tissue glycosylation	1911:1955	New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction.					
31108197	4	106	theme	lectin	756:761	arg1	histochemistry					763:776	lectin histochemistry	756:776	lectin histochemistry	756:776	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	0	107	theme	membrane	26:33	arg1	proteins					35:42	membrane proteins	26:42	membrane proteins	26:42	Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue.					
31108197	5	108	theme	high	990:993	arg1	structures					1003:1012	high mannose structures	990:1012	high mannose structures	990:1012	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	3	109	theme	cellular	408:415	arg1	function					417:424	cellular function	408:424	cellular function	408:424	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	4	110	theme	ventricle	596:604	arg1	glycosylation					557:569	glycosylation	557:569	glycosylation of the adult rat cardiac ventricle	557:604	In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling.					
31108197	7	111	theme	core	1359:1362	arg1	glycans					1380:1386	mucin core type 2 O-linked glycans	1353:1386	mucin core type 2 O-linked glycans	1353:1386	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	3	112	from	tissue	492:497	arg1	variation					443:451	variation	443:451	variation in cardiac glycosylation from neonatal tissue to maturation	443:511	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	3	112	from	tissue	492:497	arg1	glycosylation					464:476	cardiac glycosylation	456:476	cardiac glycosylation from neonatal tissue to maturation	456:511	Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined.					
31108197	5	113	theme	Sprague-Dawley	820:833	arg1	strain					835:840	the Sprague-Dawley strain	816:840	the Sprague-Dawley strain	816:840	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	5	114	theme	three-day-old	1070:1082	arg1	tissue					1093:1098	the three-day-old neonatal tissue	1066:1098	the three-day-old neonatal tissue	1066:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	7	115	link	O-linked	1438:1445	arg1	structure					1447:1455	one sulfated core type 2 O-linked structure	1413:1455	one sulfated core type 2 O-linked structure	1413:1455	The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue.					
31108197	8	116	theme	N-linked	1652:1659	arg1	glycans					1661:1667	all sialylated N-linked glycans	1637:1667	all sialylated N-linked glycans detected	1637:1676	Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac.					
31108197	2	117	theme	exclusive	226:234	arg1	Carbohydrates					212:224	Carbohydrates	212:224	Carbohydrates exclusive to cardiac neonatal tissue	212:261	Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential.					
31108197	5	118	from	expression	976:985	arg1	tissue					1093:1098	the three-day-old neonatal tissue	1066:1098	the three-day-old neonatal tissue	1066:1098	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31108197	5	119	theme	N-linked	1046:1053	arg1	glycans					1055:1061	complex N-linked glycans	1038:1061	complex N-linked glycans	1038:1061	However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue.					
31331074	2	0	theme	cartilage	368:376	arg1	degradation					378:388	protease-mediated cartilage degradation	350:388	protease-mediated cartilage degradation	350:388	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	9	1	attach	derived	1365:1371	arg2	glycans					1357:1363	various glycans	1349:1363	various glycans derived from glycoconjugates during hypertrophic differentiation	1349:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	9	1	attach	derived	1365:1371	arg1	glycoconjugates					1378:1392	glycoconjugates	1378:1392	glycoconjugates	1378:1392	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	0	2	theme	Chondrocytes	72:83	arg1	Differentiation					53:67	Late Differentiation	48:67	Late Differentiation of Chondrocytes	48:83	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.					
31331074	4	3	theme	cellular	540:547	arg1	differentiation					549:563	Dynamic cellular differentiation	532:563	Dynamic cellular differentiation	532:563	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	3	4	located	located	493:499	arg2	Glycans					481:487	Glycans	481:487	Glycans	481:487	Glycans are located on the outermost cell surface.					
31331074	3	4	located	located	493:499	arg1	surface					523:529	the outermost cell surface	504:529	the outermost cell surface	504:529	Glycans are located on the outermost cell surface.					
31331074	2	5	theme	protease-mediated	350:366	arg1	degradation					378:388	protease-mediated cartilage degradation	350:388	protease-mediated cartilage degradation	350:388	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	11	6	theme	glycan	1614:1619	arg1	alterations					1621:1631	total cellular glycan alterations	1599:1631	total cellular glycan alterations	1599:1631	Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation.					
31331074	4	7	theme	Dynamic	532:538	arg1	differentiation					549:563	Dynamic cellular differentiation	532:563	Dynamic cellular differentiation	532:563	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	6	8	theme	chondro-osteogenic	898:915	arg1	model					933:937	an insulin-induced chondro-osteogenic differentiation model	879:937	an insulin-induced chondro-osteogenic differentiation model	879:937	Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model.					
31331074	9	9	theme	Comprehensive	1265:1277	arg1	analyses					1288:1295	Comprehensive glycomic analyses	1265:1295	Comprehensive glycomic analyses	1265:1295	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	6	10	theme	insulin-induced	882:896	arg1	model					933:937	an insulin-induced chondro-osteogenic differentiation model	879:937	an insulin-induced chondro-osteogenic differentiation model	879:937	Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model.					
31331074	4	11	theme	glycan	632:637	arg1	structures					639:648	the glycan structures	628:648	the glycan structures of total cellular glycoproteins	628:680	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	5	12	theme	hypertrophy-like	794:809	arg1	changes					811:817	hypertrophy-like changes	794:817	hypertrophy-like changes	794:817	This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur.					
31331074	8	13	theme	alteration	1173:1182	arg1	clustering					1155:1164	clustering	1155:1164	clustering	1155:1164	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	8	13	theme	alteration	1173:1182	arg1	comparison					1140:1149	comparison	1140:1149	comparison	1140:1149	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	4	14	gly	glycoproteins	668:680	arg1	glycoproteins					668:680	total cellular glycoproteins	653:680	total cellular glycoproteins	653:680	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	10	15	theme	related	1464:1470	arg1	genes					1458:1462	genes	1458:1462	genes related to glycan biosynthesis and metabolic processes	1458:1517	In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations.					
31331074	9	16	from	alterations	1320:1330	arg1	levels					1339:1344	the levels	1335:1344	the levels of various glycans derived from glycoconjugates during hypertrophic differentiation	1335:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	8	17	dep	comparison	1140:1149	arg1	The					1136:1138	The	1136:1138	The	1136:1138	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	9	18	theme	glycans	1357:1363	arg1	levels					1339:1344	the levels	1335:1344	the levels of various glycans derived from glycoconjugates during hypertrophic differentiation	1335:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	10	19	theme	genes	1458:1462	arg1	expression					1444:1453	expression	1444:1453	expression of genes related to glycan biosynthesis and metabolic processes	1444:1517	In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations.					
31331074	11	20	theme	total	1599:1603	arg1	alterations					1621:1631	total cellular glycan alterations	1599:1631	total cellular glycan alterations	1599:1631	Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation.					
31331074	8	21	theme	glycans	1187:1193	arg1	alteration					1173:1182	the alteration	1169:1182	the alteration of glycans	1169:1193	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	12	22	theme	biomarkers	1858:1867	arg1	identification					1809:1822	the identification	1805:1822	the identification of diagnostic and differentiation biomarkers	1805:1867	our results will assist the identification of diagnostic and differentiation biomarkers in the future.					
31331074	1	23	theme	normal	89:94	arg1	cartilage					106:114	normal articular cartilage	89:114	normal articular cartilage	89:114	In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism.					
31331074	7	24	theme	Comprehensive	940:952	arg1	glycosaminoglycan					1020:1036	glycosaminoglycan	1020:1036	glycosaminoglycan	1020:1036	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	7	24	theme	Comprehensive	940:952	arg1	O-glycans					986:994	O-glycans	986:994	O-glycans	986:994	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	7	24	theme	Comprehensive	940:952	arg1	glycomics					954:962	Comprehensive glycomics	940:962	Comprehensive glycomics	940:962	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	7	24	theme	Comprehensive	940:952	arg1	glycosphingolipid					1043:1059	glycosphingolipid	1043:1059	glycosphingolipid	1043:1059	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	7	24	theme	Comprehensive	940:952	arg1	oligosaccharides					1002:1017	free oligosaccharides	997:1017	free oligosaccharides	997:1017	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	7	24	theme	Comprehensive	940:952	arg1	N-glycans					975:983	N-glycans	975:983	N-glycans	975:983	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	0	25	theme	Total	18:22	arg1	Glycome					33:39	the Total Cellular Glycome	14:39	the Total Cellular Glycome	14:39	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.					
31331074	12	26	theme	differentiation	1842:1856	arg1	biomarkers					1858:1867	diagnostic and differentiation biomarkers	1827:1867	diagnostic and differentiation biomarkers	1827:1867	our results will assist the identification of diagnostic and differentiation biomarkers in the future.					
31331074	8	27	theme	hypertrophy-like	1200:1215	arg1	changes					1217:1223	hypertrophy-like changes	1200:1223	hypertrophy-like changes of primary chondrocytes	1200:1247	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	9	28	theme	various	1349:1355	arg1	glycans					1357:1363	various glycans	1349:1363	various glycans derived from glycoconjugates during hypertrophic differentiation	1349:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	1	29	theme	articular	96:104	arg1	cartilage					106:114	normal articular cartilage	89:114	normal articular cartilage	89:114	In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism.					
31331074	6	30	theme	differentiation	917:931	arg1	model					933:937	an insulin-induced chondro-osteogenic differentiation model	879:937	an insulin-induced chondro-osteogenic differentiation model	879:937	Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model.					
31331074	9	31	theme	glycomic	1279:1286	arg1	analyses					1288:1295	Comprehensive glycomic analyses	1265:1295	Comprehensive glycomic analyses	1265:1295	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	11	32	theme	chondrocyte	1661:1671	arg1	hypertrophy					1673:1683	chondrocyte hypertrophy	1661:1683	chondrocyte hypertrophy	1661:1683	Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation.					
31331074	1	33	theme	low	200:202	arg1	level					204:208	a low level	198:208	a low level of metabolism	198:222	In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism.					
31331074	0	34	theme	Glycome	33:39	arg1	Alteration					0:9	Alteration	0:9	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.	0:84	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.					
31331074	5	35	theme	chondrocytes	767:778	arg1	differentiation					748:762	late differentiation	743:762	late differentiation of chondrocytes	743:778	This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur.					
31331074	9	36	theme	hypertrophic	1401:1412	arg1	differentiation					1414:1428	hypertrophic differentiation	1401:1428	hypertrophic differentiation	1401:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	10	37	theme	glycan	1475:1480	arg1	biosynthesis					1482:1493	glycan biosynthesis	1475:1493	glycan biosynthesis	1475:1493	In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations.					
31331074	2	38	theme	molecular	422:430	arg1	unclear					472:478	unclear	472:478	unclear	472:478	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	2	38	theme	molecular	422:430	arg1	mechanisms					432:441	the molecular mechanisms	418:441	the molecular mechanisms underlying these changes	418:466	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	0	39	theme	Cellular	24:31	arg1	Glycome					33:39	the Total Cellular Glycome	14:39	the Total Cellular Glycome	14:39	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.					
31331074	2	40	theme	Hypertrophy-like	225:240	arg1	changes					242:248	Hypertrophy-like changes	225:248	Hypertrophy-like changes of chondrocytes	225:264	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	6	41	theme	mouse	834:838	arg1	chondrocytes					840:851	Primary mouse chondrocytes	826:851	Primary mouse chondrocytes	826:851	Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model.					
31331074	11	42	theme	cellular	1605:1612	arg1	alterations					1621:1631	total cellular glycan alterations	1599:1631	total cellular glycan alterations	1599:1631	Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation.					
31331074	0	43	theme	Late	48:51	arg1	Differentiation					53:67	Late Differentiation	48:67	Late Differentiation of Chondrocytes	48:83	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.					
31331074	5	44	from	alterations	715:725	arg1	glycans					730:736	glycans	730:736	glycans upon late differentiation of chondrocytes	730:778	This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur.					
31331074	8	45	theme	chondrocytes	1236:1247	arg1	changes					1217:1223	hypertrophy-like changes	1200:1223	hypertrophy-like changes of primary chondrocytes	1200:1247	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	6	46	theme	Primary	826:832	arg1	chondrocytes					840:851	Primary mouse chondrocytes	826:851	Primary mouse chondrocytes	826:851	Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model.					
31331074	3	47	theme	outermost	508:516	arg1	surface					523:529	the outermost cell surface	504:529	the outermost cell surface	504:529	Glycans are located on the outermost cell surface.					
31331074	8	48	theme	primary	1228:1234	arg1	chondrocytes					1236:1247	primary chondrocytes	1228:1247	primary chondrocytes	1228:1247	The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed.					
31331074	7	49	theme	20-days	1115:1121	arg1	cultivation					1123:1133	20-days cultivation	1115:1133	20-days cultivation	1115:1133	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	3	50	theme	cell	518:521	arg1	surface					523:529	the outermost cell surface	504:529	the outermost cell surface	504:529	Glycans are located on the outermost cell surface.					
31331074	12	51	theme	diagnostic	1827:1836	arg1	biomarkers					1858:1867	diagnostic and differentiation biomarkers	1827:1867	diagnostic and differentiation biomarkers	1827:1867	our results will assist the identification of diagnostic and differentiation biomarkers in the future.					
31331074	11	52	theme	hypertrophic	1751:1762	arg1	differentiation					1764:1778	their hypertrophic differentiation	1745:1778	their hypertrophic differentiation	1745:1778	Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation.					
31331074	2	53	theme	osteoarthritis	323:336	arg1	pathogenesis					307:318	the pathogenesis	303:318	the pathogenesis of osteoarthritis	303:336	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	1	54	theme	metabolism	213:222	arg1	level					204:208	a low level	198:208	a low level of metabolism	198:222	In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism.					
31331074	5	55	theme	late	743:746	arg1	differentiation					748:762	late differentiation	743:762	late differentiation of chondrocytes	743:778	This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur.					
31331074	10	56	theme	metabolic	1499:1507	arg1	processes					1509:1517	metabolic processes	1499:1517	metabolic processes	1499:1517	In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations.					
31331074	2	57	theme	chondrocytes	253:264	arg1	changes					242:248	Hypertrophy-like changes	225:248	Hypertrophy-like changes of chondrocytes	225:264	Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear.					
31331074	9	58	theme	complementary	1306:1318	arg1	alterations					1320:1330	complementary alterations	1306:1330	complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation	1306:1428	Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation.					
31331074	4	59	theme	glycoproteins	668:680	arg1	structures					639:648	the glycan structures	628:648	the glycan structures of total cellular glycoproteins	628:680	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	10	60	theme	glycan	1553:1558	arg1	alterations					1560:1570	glycan alterations	1553:1570	glycan alterations	1553:1570	In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations.					
31331074	4	61	theme	total	653:657	arg1	glycoproteins					668:680	total cellular glycoproteins	653:680	total cellular glycoproteins	653:680	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
31331074	7	62	theme	free	997:1000	arg1	oligosaccharides					1002:1017	free oligosaccharides	997:1017	free oligosaccharides	997:1017	Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation.					
31331074	4	63	theme	cellular	659:666	arg1	glycoproteins					668:680	total cellular glycoproteins	653:680	total cellular glycoproteins	653:680	Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins.					
30708139	5	0	theme	amino	753:757	arg1	sequence					764:771	the amino acid sequence	749:771	the amino acid sequence of LH	749:777	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	5	0	theme	amino	753:757	arg1	step					731:734	the first step	721:734	the first step	721:734	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	4	1	theme	acid	703:706	arg1	sequence					708:715	amino acid sequence	697:715	amino acid sequence	697:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	1	2	theme	serum	156:160	arg1	half-life					162:170	serum half-life	156:170	serum half-life of valuable protein therapeutics	156:203	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	4	3	theme	rational	580:587	arg1	approach					599:606	A rational in silico approach	578:606	A rational in silico approach	578:606	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	0	4	theme	bioinformatics	75:88	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	4	theme	bioinformatics	75:88	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	3	5	theme	suitable	505:512	arg1	positions					514:522	the suitable positions	501:522	the suitable positions for the addition of new N-linked glycosylation sites	501:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	2	6	from	positions	324:332	arg1	backbone					349:356	the peptide backbone	337:356	the peptide backbone	337:356	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	5	7	theme	first	725:729	arg1	sequence					764:771	the amino acid sequence	749:771	the amino acid sequence of LH	749:777	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	5	7	theme	first	725:729	arg1	step					731:734	the first step	721:734	the first step	721:734	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	7	8	theme	selected	1065:1072	arg1	analogs					1074:1080	the selected analogs	1061:1080	the selected analogs	1061:1080	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	8	9	theme	experimental	1314:1325	arg1	investigations					1327:1340	further experimental investigations	1306:1340	further experimental investigations	1306:1340	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	4	10	theme	alterations	664:674	arg1	prediction					624:633	prediction	624:633	prediction of structural and functional alterations caused by changes in amino acid sequence	624:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	8	11	theme	further	1306:1312	arg1	investigations					1327:1340	further experimental investigations	1306:1340	further experimental investigations	1306:1340	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	7	12	theme	model	1117:1121	arg1	methods					1134:1140	the model evaluation methods	1113:1140	the model evaluation methods	1113:1140	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	3	13	theme	new	544:546	arg1	sites					571:575	new N-linked glycosylation sites	544:575	new N-linked glycosylation sites	544:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	9	14	theme	computational	1347:1359	arg1	strategy					1361:1368	Our computational strategy	1343:1368	Our computational strategy	1343:1368	Our computational strategy can reduce laborious and time-consuming experimental analyses of the analogs.					
30708139	4	15	theme	in	589:590	arg1	approach					599:606	A rational in silico approach	578:606	A rational in silico approach	578:606	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	4	16	theme	amino	697:701	arg1	sequence					708:715	amino acid sequence	697:715	amino acid sequence	697:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	2	17	gly	hyper-glycosylated	390:407	arg1	protein					409:415	hyper-glycosylated protein	390:415	hyper-glycosylated protein	390:415	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	8	18	theme	qualified	1225:1233	arg1	analogs					1156:1162	two analogs	1152:1162	two analogs with one additional glycosylation site	1152:1201	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	8	18	theme	qualified	1225:1233	arg1	analogs					1235:1241	the qualified analogs	1221:1241	the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations	1221:1340	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	2	19	theme	protein	409:415	arg1	generation					376:385	the generation	372:385	the generation of hyper-glycosylated protein	372:415	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	7	20	dep	Three-dimensional	1024:1040	arg1	3D					1043:1044	3D	1043:1044	3D	1043:1044	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	3	21	theme	human	433:437	arg1	LH					460:461	LH	460:461	LH	460:461	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	3	21	theme	human	433:437	arg1	hormone					451:457	human luteinizing hormone	433:457	human luteinizing hormone (LH)	433:462	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	9	22	theme	laborious	1381:1389	arg1	analyses					1423:1430	laborious and time-consuming experimental analyses	1381:1430	laborious and time-consuming experimental analyses of the analogs	1381:1445	Our computational strategy can reduce laborious and time-consuming experimental analyses of the analogs.					
30708139	1	23	theme	valuable	175:182	arg1	therapeutics					192:203	valuable protein therapeutics	175:203	valuable protein therapeutics	175:203	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	5	24	theme	acid	759:762	arg1	sequence					764:771	the amino acid sequence	749:771	the amino acid sequence of LH	749:777	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	5	24	theme	acid	759:762	arg1	step					731:734	the first step	721:734	the first step	721:734	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	4	25	theme	functional	653:662	arg1	alterations					664:674	structural and functional alterations	638:674	structural and functional alterations caused by changes in amino acid sequence	638:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	3	26	theme	sites	571:575	arg1	addition					532:539	the addition	528:539	the addition of new N-linked glycosylation sites	528:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	7	27	theme	Three-dimensional	1024:1040	arg1	structures					1047:1056	Three-dimensional (3D) structures	1024:1056	Three-dimensional (3D) structures of the selected analogs	1024:1080	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	1	28	theme	protein	184:190	arg1	therapeutics					192:203	valuable protein therapeutics	175:203	valuable protein therapeutics	175:203	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	0	29	theme	Rational	0:7	arg1	design					9:14	Rational design	0:14	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).	0:99	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	8	30	theme	glycosylation	1184:1196	arg1	site					1198:1201	one additional glycosylation site	1169:1201	one additional glycosylation site	1169:1201	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	2	31	theme	desirable	314:322	arg1	positions					324:332	desirable positions	314:332	desirable positions in the peptide backbone	314:356	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	6	32	theme	acceptable	972:981	arg1	ones					983:986	then the four acceptable ones	958:986	then the four acceptable ones	958:986	This exploration led to the identification of 38 potential N-glycan sites, and then the four acceptable ones were selected for further analysis.					
30708139	1	33	theme	therapeutics	192:203	arg1	half-life					162:170	serum half-life	156:170	serum half-life of valuable protein therapeutics	156:203	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	0	34	theme	hyper-glycosylated	19:36	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	34	theme	hyper-glycosylated	19:36	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	35	gly	hyper-glycosylated	19:36	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	35	gly	hyper-glycosylated	19:36	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	3	36	theme	N-linked	548:555	arg1	sites					571:575	new N-linked glycosylation sites	544:575	new N-linked glycosylation sites	544:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	7	37	theme	analogs	1074:1080	arg1	structures					1047:1056	Three-dimensional (3D) structures	1024:1056	Three-dimensional (3D) structures of the selected analogs	1024:1080	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	2	38	gly	N-glycosylation	257:271	arg2	site					273:276	new N-glycosylation site	253:276	new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro)	253:307	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	6	39	theme	sites	947:951	arg1	identification					907:920	the identification	903:920	the identification of 38 potential N-glycan sites	903:951	This exploration led to the identification of 38 potential N-glycan sites, and then the four acceptable ones were selected for further analysis.					
30708139	8	40	with	analogs	1156:1162	arg1	site					1198:1201	one additional glycosylation site	1169:1201	one additional glycosylation site	1169:1201	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	3	41	theme	glycosylation	557:569	arg1	sites					571:575	new N-linked glycosylation sites	544:575	new N-linked glycosylation sites	544:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	5	42	theme	N-glycosylation	856:870	arg1	sites					872:876	new N-glycosylation sites	852:876	new N-glycosylation sites	852:876	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	0	43	theme	luteinizing	44:54	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	43	theme	luteinizing	44:54	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	6	44	theme	N-glycan	938:945	arg1	sites					947:951	38 potential N-glycan sites	925:951	38 potential N-glycan sites	925:951	This exploration led to the identification of 38 potential N-glycan sites, and then the four acceptable ones were selected for further analysis.					
30708139	4	45	dep	in	589:590	arg1	silico					592:597	silico	592:597	silico	592:597	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	2	46	dep	site	273:276	arg1	Asn-X-Thr/Ser					279:291	Asn-X-Thr/Ser	279:291	Asn-X-Thr/Ser	279:291	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	5	47	theme	Asn	827:829	arg1	Thr					838:840	Asn or/and Thr	827:840	Asn or/and Thr	827:840	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	0	48	theme	human	38:42	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	48	theme	human	38:42	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	6	49	theme	potential	928:936	arg1	sites					947:951	38 potential N-glycan sites	925:951	38 potential N-glycan sites	925:951	This exploration led to the identification of 38 potential N-glycan sites, and then the four acceptable ones were selected for further analysis.					
30708139	7	50	theme	evaluation	1123:1132	arg1	methods					1134:1140	the model evaluation methods	1113:1140	the model evaluation methods	1113:1140	Three-dimensional (3D) structures of the selected analogs were generated and examined by the model evaluation methods.					
30708139	5	51	theme	desirable	791:799	arg1	positions					801:809	desirable positions	791:809	desirable positions	791:809	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	5	52	theme	or/and	831:836	arg1	Thr					838:840	Asn or/and Thr	827:840	Asn or/and Thr	827:840	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	4	53	theme	structural	638:647	arg1	alterations					664:674	structural and functional alterations	638:674	structural and functional alterations caused by changes in amino acid sequence	638:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	0	54	theme	analogs	64:70	arg1	design					9:14	Rational design	0:14	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).	0:99	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	8	55	gly	glycosylation	1184:1196	arg2	site					1198:1201	one additional glycosylation site	1169:1201	one additional glycosylation site	1169:1201	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	8	55	gly	glycosylation	1184:1196	arg2	one					1169:1171	one	1169:1171	one	1169:1171	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	6	56	theme	further	1006:1012	arg1	analysis					1014:1021	further analysis	1006:1021	further analysis	1006:1021	This exploration led to the identification of 38 potential N-glycan sites, and then the four acceptable ones were selected for further analysis.					
30708139	3	57	theme	luteinizing	439:449	arg1	LH					460:461	LH	460:461	LH	460:461	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	3	57	theme	luteinizing	439:449	arg1	hormone					451:457	human luteinizing hormone	433:457	human luteinizing hormone (LH)	433:462	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	9	58	theme	analogs	1439:1445	arg1	analyses					1423:1430	laborious and time-consuming experimental analyses	1381:1430	laborious and time-consuming experimental analyses of the analogs	1381:1445	Our computational strategy can reduce laborious and time-consuming experimental analyses of the analogs.					
30708139	8	59	theme	LH	1274:1275	arg1	hyper-glycosylation					1247:1265	hyper-glycosylation	1247:1265	hyper-glycosylation of the LH, which can be considered for further experimental investigations	1247:1340	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	0	60	theme	hormone	56:62	arg1	approach					90:97	a bioinformatics approach	73:97	a bioinformatics approach	73:97	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	0	60	theme	hormone	56:62	arg1	analogs					64:70	hyper-glycosylated human luteinizing hormone analogs	19:70	hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach)	19:98	Rational design of hyper-glycosylated human luteinizing hormone analogs (a bioinformatics approach).					
30708139	5	61	gly	N-glycosylation	856:870	arg2	sites					872:876	new N-glycosylation sites	852:876	new N-glycosylation sites	852:876	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	2	62	theme	glycoengineering	220:235	arg1	aspect					210:215	One aspect	206:215	One aspect of glycoengineering	206:235	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	8	63	theme	additional	1173:1182	arg1	site					1198:1201	one additional glycosylation site	1169:1201	one additional glycosylation site	1169:1201	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	2	64	theme	peptide	341:347	arg1	backbone					349:356	the peptide backbone	337:356	the peptide backbone	337:356	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	9	65	theme	experimental	1410:1421	arg1	analyses					1423:1430	laborious and time-consuming experimental analyses	1381:1430	laborious and time-consuming experimental analyses of the analogs	1381:1445	Our computational strategy can reduce laborious and time-consuming experimental analyses of the analogs.					
30708139	1	66	theme	used	132:135	arg1	approach					137:144	a recently used approach	121:144	a recently used approach to extend serum half-life of valuable protein therapeutics	121:203	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	1	66	theme	used	132:135	arg1	Glycoengineering					101:116	Glycoengineering	101:116	Glycoengineering	101:116	Glycoengineering is a recently used approach to extend serum half-life of valuable protein therapeutics.					
30708139	5	67	theme	new	852:854	arg1	sites					872:876	new N-glycosylation sites	852:876	new N-glycosylation sites	852:876	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	8	68	gly	hyper-glycosylation	1247:1265	arg1	LH					1274:1275	the LH	1270:1275	the LH	1270:1275	Finally, two analogs with one additional glycosylation site were suggested as the qualified analogs for hyper-glycosylation of the LH, which can be considered for further experimental investigations.					
30708139	3	69	gly	glycosylation	557:569	arg2	sites					571:575	new N-linked glycosylation sites	544:575	new N-linked glycosylation sites	544:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	3	70	link	N-linked	548:555	arg1	sites					571:575	new N-linked glycosylation sites	544:575	new N-linked glycosylation sites	544:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	4	71	from	changes	686:692	arg1	sequence					708:715	amino acid sequence	697:715	amino acid sequence	697:715	A rational in silico approach was applied for prediction of structural and functional alterations caused by changes in amino acid sequence.					
30708139	2	72	theme	N-glycosylation	257:271	arg1	site					273:276	new N-glycosylation site	253:276	new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro)	253:307	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	3	73	theme	positions	514:522	arg1	identification					483:496	identification	483:496	identification of the suitable positions for the addition of new N-linked glycosylation sites	483:575	In this study, human luteinizing hormone (LH) was considered for identification of the suitable positions for the addition of new N-linked glycosylation sites.					
30708139	2	74	theme	hyper-glycosylated	390:407	arg1	protein					409:415	hyper-glycosylated protein	390:415	hyper-glycosylated protein	390:415	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	5	75	theme	LH	776:777	arg1	sequence					764:771	the amino acid sequence	749:771	the amino acid sequence of LH	749:777	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	5	75	theme	LH	776:777	arg1	step					731:734	the first step	721:734	the first step	721:734	As the first step, we explored the amino acid sequence of LH to find out desirable positions for introducing Asn or/and Thr to create new N-glycosylation sites.					
30708139	2	76	theme	new	253:255	arg1	site					273:276	new N-glycosylation site	253:276	new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro)	253:307	One aspect of glycoengineering is to introduce new N-glycosylation site (Asn-X-Thr/Ser, where X ≠ Pro) into desirable positions in the peptide backbone, resulting in the generation of hyper-glycosylated protein.					
30708139	9	77	theme	time-consuming	1395:1408	arg1	analyses					1423:1430	laborious and time-consuming experimental analyses	1381:1430	laborious and time-consuming experimental analyses of the analogs	1381:1445	Our computational strategy can reduce laborious and time-consuming experimental analyses of the analogs.					
30935980	0	0	theme	beetle	72:77	arg1	larva					79:83	the Colorado potato beetle larva	52:83	the Colorado potato beetle larva (Leptinotarsa decemlineata)	52:111	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	0	theme	beetle	72:77	arg1	decemlineata					99:110	Leptinotarsa decemlineata	86:110	Leptinotarsa decemlineata	86:110	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	1	from	profile	13:19	arg1	larva					79:83	the Colorado potato beetle larva	52:83	the Colorado potato beetle larva (Leptinotarsa decemlineata)	52:111	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	1	from	profile	13:19	arg1	decemlineata					99:110	Leptinotarsa decemlineata	86:110	Leptinotarsa decemlineata	86:110	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	5	2	theme	beetle	606:611	arg1	PM					580:581	the PM	576:581	the PM of the Colorado potato beetle (CPB)	576:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	8	3	theme	PM	1023:1024	arg1	N-glycome					1006:1014	the N-glycome	1002:1014	the N-glycome of the PM of a pest insect	1002:1041	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30935980	5	4	from	present	565:571	arg1	PM					580:581	the PM	576:581	the PM of the Colorado potato beetle (CPB)	576:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	1	5	theme	proteoglycan	223:234	arg1	matrix					236:241	a proteoglycan matrix	221:241	a proteoglycan matrix together with chitin	221:262	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	0	6	theme	potato	65:70	arg1	larva					79:83	the Colorado potato beetle larva	52:83	the Colorado potato beetle larva (Leptinotarsa decemlineata)	52:111	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	6	theme	potato	65:70	arg1	decemlineata					99:110	Leptinotarsa decemlineata	86:110	Leptinotarsa decemlineata	86:110	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	4	7	theme	first	491:495	arg1	time					497:500	the first time	487:500	the first time	487:500	Here we studied for the first time the N-glycome of the PM.					
30935980	8	8	theme	first	986:990	arg1	report					992:997	the first report	982:997	the first report on the N-glycome of the PM of a pest insect	982:1041	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30935980	8	8	theme	first	986:990	arg1	data					973:976	these data	967:976	these data	967:976	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30935980	5	9	theme	present	565:571	arg1	structures					554:563	the N-glycan structures	541:563	the N-glycan structures present in the PM of the Colorado potato beetle (CPB)	541:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	8	10	theme	pest	1031:1034	arg1	insect					1036:1041	a pest insect	1029:1041	a pest insect	1029:1041	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30935980	9	11	theme	importance	1094:1103	arg1	study					1081:1085	the study	1077:1085	the study of the importance of N-glycosylation in the function and structure of the PM	1077:1162	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	7	12	from	proteins	930:937	arg1	PM					946:947	the PM	942:947	the PM	942:947	The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM.					
30935980	6	13	theme	cells	829:833	arg1	transcriptome					794:806	the transcriptome	790:806	the transcriptome of epithelial midgut cells	790:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	9	14	from	structure	1144:1152	arg1	study					1081:1085	the study	1077:1085	the study of the importance of N-glycosylation in the function and structure of the PM	1077:1162	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	10	15	theme	pest	1254:1257	arg1	control					1259:1265	pest control	1254:1265	pest control	1254:1265	In addition, the data can help to find novel targets and design better biopesticides for pest control.					
30935980	7	16	theme	N-glycan	896:903	arg1	profile					905:911	the N-glycan profile	892:911	the N-glycan profile resolved for the proteins in the PM	892:947	The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM.					
30935980	9	17	from	importance	1094:1103	arg1	function					1131:1138	function	1131:1138	function	1131:1138	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	9	17	from	importance	1094:1103	arg1	structure					1144:1152	structure	1144:1152	structure	1144:1152	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	6	18	attach	presence	733:740	arg2	NGRGs					780:784	NGRGs	780:784	NGRGs	780:784	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	6	18	attach	presence	733:740	arg2	genes					773:777	the N-glycosylation related genes	745:777	the N-glycosylation related genes (NGRGs)	745:785	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	6	18	attach	presence	733:740	arg1	transcriptome					794:806	the transcriptome	790:806	the transcriptome of epithelial midgut cells	790:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	6	19	theme	midgut	822:827	arg1	cells					829:833	epithelial midgut cells	811:833	epithelial midgut cells	811:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	2	20	from	contents	320:327	arg1	epithelium					289:298	the midgut epithelium	278:298	the midgut epithelium from the intestinal contents	278:327	It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food.					
30935980	0	21	theme	Leptinotarsa	86:97	arg1	larva					79:83	the Colorado potato beetle larva	52:83	the Colorado potato beetle larva (Leptinotarsa decemlineata)	52:111	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	21	theme	Leptinotarsa	86:97	arg1	decemlineata					99:110	Leptinotarsa decemlineata	86:110	Leptinotarsa decemlineata	86:110	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	6	22	theme	epithelial	811:820	arg1	cells					829:833	epithelial midgut cells	811:833	epithelial midgut cells	811:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	9	23	theme	PM	1161:1162	arg1	function					1131:1138	function	1131:1138	function	1131:1138	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	9	23	theme	PM	1161:1162	arg1	structure					1144:1152	structure	1144:1152	structure	1144:1152	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	7	24	theme	identified	867:876	arg1	genes					878:882	the identified genes	863:882	the identified genes	863:882	The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM.					
30935980	2	25	theme	food	364:367	arg1	digestion					351:359	the digestion	347:359	the digestion of food	347:367	It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food.					
30935980	0	26	theme	N-glycan	4:11	arg1	profile					13:19	The N-glycan profile	0:19	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).	0:112	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	8	27	theme	insect	1036:1041	arg1	PM					1023:1024	the PM	1019:1024	the PM of a pest insect	1019:1041	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30935980	5	28	theme	Colorado	590:597	arg1	CPB					614:616	CPB	614:616	CPB	614:616	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	5	28	theme	Colorado	590:597	arg1	beetle					606:611	the Colorado potato beetle	586:611	the Colorado potato beetle (CPB)	586:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	7	29	theme	genes	878:882	arg1	activities					849:858	The presumed activities	836:858	The presumed activities of the identified genes	836:882	The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM.					
30935980	5	30	attach	present	565:571	arg2	structures					554:563	the N-glycan structures	541:563	the N-glycan structures present in the PM of the Colorado potato beetle (CPB)	541:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	5	30	attach	present	565:571	arg1	PM					580:581	the PM	576:581	the PM of the Colorado potato beetle (CPB)	576:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	4	31	theme	PM	523:524	arg1	N-glycome					506:514	the N-glycome	502:514	the N-glycome of the PM	502:524	Here we studied for the first time the N-glycome of the PM.					
30935980	5	32	theme	potato	599:604	arg1	CPB					614:616	CPB	614:616	CPB	614:616	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	5	32	theme	potato	599:604	arg1	beetle					606:611	the Colorado potato beetle	586:611	the Colorado potato beetle (CPB)	586:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	5	33	theme	N-glycan	545:552	arg1	structures					554:563	the N-glycan structures	541:563	the N-glycan structures present in the PM of the Colorado potato beetle (CPB)	541:617	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	3	34	theme	protective	395:404	arg1	barrier					406:412	a protective barrier	393:412	a protective barrier against abrasive particles and microbial infections	393:464	Furthermore it acts as a protective barrier against abrasive particles and microbial infections.					
30935980	3	34	theme	protective	395:404	arg1	it					382:383	it	382:383	it	382:383	Furthermore it acts as a protective barrier against abrasive particles and microbial infections.					
30935980	6	35	theme	N-glycan	712:719	arg1	data					721:724	the N-glycan data	708:724	the N-glycan data	708:724	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	2	36	theme	midgut	282:287	arg1	epithelium					289:298	the midgut epithelium	278:298	the midgut epithelium from the intestinal contents	278:327	It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food.					
30935980	6	37	theme	N-glycosylation	749:763	arg1	NGRGs					780:784	NGRGs	780:784	NGRGs	780:784	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	6	37	theme	N-glycosylation	749:763	arg1	genes					773:777	the N-glycosylation related genes	745:777	the N-glycosylation related genes (NGRGs)	745:785	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	3	38	theme	microbial	445:453	arg1	infections					455:464	microbial infections	445:464	microbial infections	445:464	Furthermore it acts as a protective barrier against abrasive particles and microbial infections.					
30935980	0	39	theme	peritrophic	28:38	arg1	membrane					40:47	the peritrophic membrane	24:47	the peritrophic membrane	24:47	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	5	40	theme	MALDI-TOF	652:660	arg1	spectrometry					667:678	MALDI-TOF mass spectrometry	652:678	MALDI-TOF mass spectrometry	652:678	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	1	41	theme	non-cellular	156:167	arg1	membrane					137:144	The insect peritrophic membrane	114:144	The insect peritrophic membrane (PM)	114:149	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	1	41	theme	non-cellular	156:167	arg1	structure					169:177	a non-cellular structure	154:177	a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin	154:262	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	3	42	theme	abrasive	422:429	arg1	particles					431:439	abrasive particles	422:439	abrasive particles	422:439	Furthermore it acts as a protective barrier against abrasive particles and microbial infections.					
30935980	9	43	from	study	1081:1085	arg1	function					1131:1138	function	1131:1138	function	1131:1138	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	9	43	from	study	1081:1085	arg1	structure					1144:1152	structure	1144:1152	structure	1144:1152	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	5	44	theme	mass	662:665	arg1	spectrometry					667:678	MALDI-TOF mass spectrometry	652:678	MALDI-TOF mass spectrometry	652:678	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	1	45	theme	secreted	191:198	arg1	proteins					200:207	secreted proteins	191:207	secreted proteins imbedded in a proteoglycan matrix together with chitin	191:262	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	9	46	from	function	1131:1138	arg1	study					1081:1085	the study	1077:1085	the study of the importance of N-glycosylation in the function and structure of the PM	1077:1162	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	10	47	theme	novel	1204:1208	arg1	targets					1210:1216	novel targets	1204:1216	novel targets	1204:1216	In addition, the data can help to find novel targets and design better biopesticides for pest control.					
30935980	0	48	theme	membrane	40:47	arg1	profile					13:19	The N-glycan profile	0:19	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).	0:112	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	7	49	theme	presumed	840:847	arg1	activities					849:858	The presumed activities	836:858	The presumed activities of the identified genes	836:882	The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM.					
30935980	9	50	theme	N-glycosylation	1108:1122	arg1	importance					1094:1103	the importance	1090:1103	the importance of N-glycosylation in the function and structure of the PM	1090:1162	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	5	51	theme	fourth	626:631	arg1	stage					640:644	the fourth larval stage	622:644	the fourth larval stage	622:644	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	2	52	theme	intestinal	309:318	arg1	contents					320:327	the intestinal contents	305:327	the intestinal contents	305:327	It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food.					
30935980	9	53	dep	function	1131:1138	arg1	the					1127:1129	the	1127:1129	the	1127:1129	These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM.					
30935980	5	54	theme	larval	633:638	arg1	stage					640:644	the fourth larval stage	622:644	the fourth larval stage	622:644	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	6	55	theme	genes	773:777	arg1	presence					733:740	the presence	729:740	the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells	729:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	1	56	theme	insect	118:123	arg1	membrane					137:144	The insect peritrophic membrane	114:144	The insect peritrophic membrane (PM)	114:149	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	1	56	theme	insect	118:123	arg1	structure					169:177	a non-cellular structure	154:177	a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin	154:262	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	1	56	theme	insect	118:123	arg1	PM					147:148	PM	147:148	PM	147:148	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	0	57	theme	Colorado	56:63	arg1	larva					79:83	the Colorado potato beetle larva	52:83	the Colorado potato beetle larva (Leptinotarsa decemlineata)	52:111	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	0	57	theme	Colorado	56:63	arg1	decemlineata					99:110	Leptinotarsa decemlineata	86:110	Leptinotarsa decemlineata	86:110	The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata).					
30935980	6	58	theme	related	765:771	arg1	NGRGs					780:784	NGRGs	780:784	NGRGs	780:784	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	6	58	theme	related	765:771	arg1	genes					773:777	the N-glycosylation related genes	745:777	the N-glycosylation related genes (NGRGs)	745:785	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	10	59	theme	better	1229:1234	arg1	biopesticides					1236:1248	better biopesticides	1229:1248	better biopesticides for pest control	1229:1265	In addition, the data can help to find novel targets and design better biopesticides for pest control.					
30935980	1	60	theme	peritrophic	125:135	arg1	membrane					137:144	The insect peritrophic membrane	114:144	The insect peritrophic membrane (PM)	114:149	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	1	60	theme	peritrophic	125:135	arg1	structure					169:177	a non-cellular structure	154:177	a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin	154:262	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	1	60	theme	peritrophic	125:135	arg1	PM					147:148	PM	147:148	PM	147:148	The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin.					
30935980	6	61	from	presence	733:740	arg1	transcriptome					794:806	the transcriptome	790:806	the transcriptome of epithelial midgut cells	790:833	In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells.					
30935980	5	62	from	PM	580:581	arg1	present					565:571	present	565:571	present	565:571	We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry.					
30935980	8	63	from	report	992:997	arg1	N-glycome					1006:1014	the N-glycome	1002:1014	the N-glycome of the PM of a pest insect	1002:1041	To our knowledge these data are the first report on the N-glycome of the PM of a pest insect.					
30622255	5	0	theme	phosphorylcholine	831:847	arg1	residues					849:856	antennal fucose and phosphorylcholine residues	811:856	antennal fucose and phosphorylcholine residues	811:856	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	3	1	theme	glucuronylated	482:495	arg1	structures					497:506	glucuronylated structures	482:506	glucuronylated structures	482:506	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	2	2	theme	four-dimensional	203:218	arg1	analysis					220:227	a four-dimensional analysis	201:227	a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions	201:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	6	3	dep	D.	1030:1031	arg1	immitis					1033:1039	D. immitis	1030:1039	D. immitis	1030:1039	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	6	4	theme	D.	1030:1031	arg1	N-glycans					1017:1025	the N-glycans	1013:1025	the N-glycans of D. immitis	1013:1039	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	5	5	theme	Glycan	680:685	arg1	data					693:696	Glycan array data	680:696	Glycan array data	680:696	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	6	6	theme	host	1107:1110	arg1	immunomodulation					1083:1098	immunomodulation	1083:1098	immunomodulation of the host	1083:1110	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	1	7	theme	canine	72:77	arg1	nematode					141:148	a mosquito-borne parasitic nematode	114:148	a mosquito-borne parasitic nematode whose range is extending due to climate change	114:195	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	1	7	theme	canine	72:77	arg1	Dirofilaria					90:100	Dirofilaria	90:100	Dirofilaria	90:100	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	1	7	theme	canine	72:77	arg1	heartworm					79:87	The canine heartworm	68:87	The canine heartworm (Dirofilaria immitis)	68:109	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	0	8	theme	modified	7:14	arg1	N-glycans					33:41	Highly modified and immunoactive N-glycans	0:41	Highly modified and immunoactive N-glycans of the canine heartworm.	0:66	Highly modified and immunoactive N-glycans of the canine heartworm.					
30622255	6	9	contain	contain	1041:1047	arg2	features					1049:1056	features	1049:1056	features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance	1049:1161	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	6	9	contain	contain	1041:1047	arg1	N-glycans					1017:1025	the N-glycans	1013:1025	the N-glycans of D. immitis	1013:1039	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	6	10	theme	immune	1143:1148	arg1	surveillance					1150:1161	immune surveillance	1143:1161	immune surveillance	1143:1161	Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.					
30622255	5	11	theme	antennal	811:818	arg1	residues					849:856	antennal fucose and phosphorylcholine residues	811:856	antennal fucose and phosphorylcholine residues	811:856	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	2	12	theme	enzymatic	301:309	arg1	digestions					311:320	enzymatic digestions	301:320	enzymatic digestions	301:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	2	13	theme	complexity	368:377	arg1	N-glycome					341:349	an N-glycome	338:349	an N-glycome of unprecedented complexity	338:377	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	0	14	theme	immunoactive	20:31	arg1	N-glycans					33:41	Highly modified and immunoactive N-glycans	0:41	Highly modified and immunoactive N-glycans of the canine heartworm.	0:66	Highly modified and immunoactive N-glycans of the canine heartworm.					
30622255	5	15	theme	binding	791:797	arg1	lectin					799:804	mannose binding lectin	783:804	mannose binding lectin	783:804	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	2	16	from	HPLC	239:242	arg1	combination					271:281	combination	271:281	combination with chemical and enzymatic digestions	271:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	1	17	theme	climate	182:188	arg1	change					190:195	climate change	182:195	climate change	182:195	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	5	18	theme	neutral	712:718	arg1	glycans					720:726	the neutral glycans	708:726	the neutral glycans	708:726	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	1	19	dep	Dirofilaria	90:100	arg1	immitis					102:108	Dirofilaria immitis	90:108	Dirofilaria immitis	90:108	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	3	20	theme	up	403:404	arg1	N-glycans					390:398	N-glycans	390:398	N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures	390:506	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	5	21	from	IgM	761:763	arg1	sera					772:775	dog sera	768:775	dog sera	768:775	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	2	22	from	MALDI-TOF-MS	245:256	arg1	combination					271:281	combination	271:281	combination with chemical and enzymatic digestions	271:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	3	23	theme	long	432:435	arg1	repeats					462:468	long fucosylated HexNAc-based repeats	432:468	long fucosylated HexNAc-based repeats	432:468	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	5	24	theme	mannose	783:789	arg1	lectin					799:804	mannose binding lectin	783:804	mannose binding lectin	783:804	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	5	25	theme	fucose	820:825	arg1	residues					849:856	antennal fucose and phosphorylcholine residues	811:856	antennal fucose and phosphorylcholine residues	811:856	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	3	26	theme	fucosylated	437:447	arg1	repeats					462:468	long fucosylated HexNAc-based repeats	432:468	long fucosylated HexNAc-based repeats	432:468	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	0	27	theme	canine	50:55	arg1	heartworm					57:65	the canine heartworm	46:65	the canine heartworm	46:65	Highly modified and immunoactive N-glycans of the canine heartworm.					
30622255	5	28	theme	reactivity	887:896	arg1	pattern					876:882	this pattern	871:882	this pattern of reactivity	871:896	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	5	29	theme	array	687:691	arg1	data					693:696	Glycan array data	680:696	Glycan array data	680:696	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	5	30	theme	dog	768:770	arg1	sera					772:775	dog sera	768:775	dog sera	768:775	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	3	31	gly	fucosylated	437:447	arg1	repeats					462:468	long fucosylated HexNAc-based repeats	432:468	long fucosylated HexNAc-based repeats	432:468	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	5	32	theme	mammalian	914:922	arg1	protein					935:941	mammalian C-reactive protein	914:941	mammalian C-reactive protein	914:941	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	5	33	theme	C-reactive	924:933	arg1	protein					935:941	mammalian C-reactive protein	914:941	mammalian C-reactive protein	914:941	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	1	34	theme	parasitic	131:139	arg1	nematode					141:148	a mosquito-borne parasitic nematode	114:148	a mosquito-borne parasitic nematode whose range is extending due to climate change	114:195	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	1	34	theme	parasitic	131:139	arg1	heartworm					79:87	The canine heartworm	68:87	The canine heartworm (Dirofilaria immitis)	68:109	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	5	35	theme	complement	978:987	arg1	C1q					999:1001	the complement component C1q	974:1001	the complement component C1q	974:1001	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	2	36	from	MS/MS	262:266	arg1	combination					271:281	combination	271:281	combination with chemical and enzymatic digestions	271:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	4	37	theme	anionic	614:620	arg1	N-glycans					622:630	anionic N-glycans	614:630	anionic N-glycans	614:630	While some modifications including LacdiNAc, chitobiose, α1,3-fucose and phosphorylcholine are familiar, anionic N-glycans have previously not been reported in nematodes.					
30622255	3	38	contain	contain	424:430	arg2	repeats					462:468	long fucosylated HexNAc-based repeats	432:468	long fucosylated HexNAc-based repeats	432:468	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	3	38	contain	contain	424:430	arg1	7000 Da					409:415	7000 Da	409:415	7000 Da	409:415	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	3	38	contain	contain	424:430	arg2	structures					497:506	glucuronylated structures	482:506	glucuronylated structures	482:506	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	5	39	theme	component	989:997	arg1	C1q					999:1001	the complement component C1q	974:1001	the complement component C1q	974:1001	Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q.					
30622255	1	40	theme	mosquito-borne	116:129	arg1	nematode					141:148	a mosquito-borne parasitic nematode	114:148	a mosquito-borne parasitic nematode whose range is extending due to climate change	114:195	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	1	40	theme	mosquito-borne	116:129	arg1	heartworm					79:87	The canine heartworm	68:87	The canine heartworm (Dirofilaria immitis)	68:109	The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change.					
30622255	3	41	theme	HexNAc-based	449:460	arg1	repeats					462:468	long fucosylated HexNAc-based repeats	432:468	long fucosylated HexNAc-based repeats	432:468	We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures.					
30622255	2	42	theme	unprecedented	354:366	arg1	complexity					368:377	unprecedented complexity	354:377	unprecedented complexity	354:377	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	0	43	theme	heartworm	57:65	arg1	N-glycans					33:41	Highly modified and immunoactive N-glycans	0:41	Highly modified and immunoactive N-glycans of the canine heartworm.	0:66	Highly modified and immunoactive N-glycans of the canine heartworm.					
30622255	2	44	with	combination	271:281	arg1	chemical					288:295	chemical	288:295	chemical	288:295	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
30622255	2	44	with	combination	271:281	arg1	digestions					311:320	enzymatic digestions	301:320	enzymatic digestions	301:320	In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity.					
29790907	3	0	theme	high	339:342	arg1	spectrometry					360:371	high resolution mass spectrometry	339:371	high resolution mass spectrometry	339:371	Accurate study of glycans require high resolution mass spectrometry.					
29790907	11	1	theme	Bioinformatics	1840:1853	arg1	online					1855:1860	Bioinformatics online	1840:1860	Bioinformatics online	1840:1860	Supplementary information Supplementary data are available at Bioinformatics online.					
29790907	11	2	theme	information	1792:1802	arg1	data					1818:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data are available at Bioinformatics online.					
29790907	7	3	theme	likelihood	969:978	arg1	functions					988:996	likelihood scoring functions	969:996	likelihood scoring functions based on glycan chemical properties	969:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	3	4	theme	resolution	344:353	arg1	spectrometry					360:371	high resolution mass spectrometry	339:371	high resolution mass spectrometry	339:371	Accurate study of glycans require high resolution mass spectrometry.					
29790907	4	5	theme	automated	563:571	arg1	tools					573:577	automated tools	563:577	automated tools	563:577	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	6	6	theme	biosynthetic	886:897	arg1	relationships					907:919	biosynthetic network relationships	886:919	biosynthetic network relationships among glycans	886:933	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	5	7	theme	related	704:710	arg1	glycans					712:718	related glycans	704:718	related glycans	704:718	Existing tools for interpreting the resulting data do not take into account related glycans when evaluating individual observations, limiting their sensitivity.					
29790907	9	8	theme	heparan	1531:1537	arg1	sulfate					1539:1545	heparan sulfate	1531:1545	heparan sulfate	1531:1545	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	7	9	dep	smooth	1143:1148	arg1	achieve					1174:1180	achieve	1174:1180	achieve a consistent and more representative solution	1174:1226	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	2	10	theme	Liquid	172:177	arg1	LC-MS					221:225	LC-MS	221:225	LC-MS	221:225	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	10	theme	Liquid	172:177	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	10	theme	Liquid	172:177	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	1	11	theme	Motivation	60:69	arg1	Glycosylation					71:83	Motivation Glycosylation	60:83	Motivation Glycosylation	60:83	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	11	12	from	online	1855:1860	arg1	available					1827:1835	available	1827:1835	available	1827:1835	Supplementary information Supplementary data are available at Bioinformatics online.					
29790907	5	13	theme	resulting	664:672	arg1	data					674:677	the resulting data	660:677	the resulting data	660:677	Existing tools for interpreting the resulting data do not take into account related glycans when evaluating individual observations, limiting their sensitivity.					
29790907	4	14	theme	biological	531:540	arg1	molecules					542:550	biological molecules	531:550	biological molecules	531:550	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	7	15	theme	representative	1204:1217	arg1	solution					1219:1226	a consistent and more representative solution	1182:1226	a consistent and more representative solution	1182:1226	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	4	16	theme	intricate	406:414	arg1	sub-structures					416:429	intricate sub-structures	406:429	intricate sub-structures that encode mass and abundance	406:460	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	16	theme	intricate	406:414	arg1	mass					443:446	mass	443:446	mass	443:446	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	16	theme	intricate	406:414	arg1	abundance					452:460	abundance	452:460	abundance	452:460	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	2	17	theme	biological	285:294	arg1	samples					296:302	complex biological samples	277:302	complex biological samples	277:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	6	18	from	data	868:871	arg1	compositions					844:855	glycan compositions	837:855	glycan compositions from LC-MS data	837:871	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	8	19	theme	glycan	1278:1283	arg1	compositions					1285:1296	more glycan compositions	1273:1296	more glycan compositions	1273:1296	Our method was able to identify as many, or more glycan compositions compared to previous approaches, and demonstrated greater sensitivity with regularization.					
29790907	10	20	theme	Executable	1659:1668	arg1	http					1670:1673	Executable http	1659:1673	Executable http	1659:1673	Availability and implementation Built Executable http://www.bumc.bu.edu/msr/glycresoft/ and Source Code: https://github.com/BostonUniversityCBMS/glycresoft.					
29790907	7	21	theme	consistent	1184:1193	arg1	solution					1219:1226	a consistent and more representative solution	1182:1226	a consistent and more representative solution	1182:1226	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	8	22	theme	more	1273:1276	arg1	compositions					1285:1296	more glycan compositions	1273:1296	more glycan compositions	1273:1296	Our method was able to identify as many, or more glycan compositions compared to previous approaches, and demonstrated greater sensitivity with regularization.					
29790907	6	23	theme	network	899:905	arg1	relationships					907:919	biosynthetic network relationships	886:919	biosynthetic network relationships among glycans	886:933	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	5	24	theme	Existing	628:635	arg1	tools					637:641	Existing tools	628:641	Existing tools for interpreting the resulting data	628:677	Existing tools for interpreting the resulting data do not take into account related glycans when evaluating individual observations, limiting their sensitivity.					
29790907	8	25	theme	greater	1348:1354	arg1	sensitivity					1356:1366	greater sensitivity	1348:1366	greater sensitivity	1348:1366	Our method was able to identify as many, or more glycan compositions compared to previous approaches, and demonstrated greater sensitivity with regularization.					
29790907	0	26	theme	smoothing	23:31	arg1	Application					0:10	Application	0:10	Application of network smoothing to glycan LC-MS profiling	0:57	Application of network smoothing to glycan LC-MS profiling.					
29790907	7	27	theme	glycan	1007:1012	arg1	properties					1023:1032	glycan chemical properties	1007:1032	glycan chemical properties	1007:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	6	28	theme	glycan	837:842	arg1	compositions					844:855	glycan compositions	837:855	glycan compositions from LC-MS data	837:871	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	8	29	theme	previous	1310:1317	arg1	approaches					1319:1328	previous approaches	1310:1328	previous approaches	1310:1328	Our method was able to identify as many, or more glycan compositions compared to previous approaches, and demonstrated greater sensitivity with regularization.					
29790907	7	30	theme	Laplacian	1051:1059	arg1	regularization					1061:1074	network Laplacian regularization	1043:1074	network Laplacian regularization	1043:1074	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	0	31	theme	network	15:21	arg1	smoothing					23:31	network smoothing	15:31	network smoothing	15:31	Application of network smoothing to glycan LC-MS profiling.					
29790907	4	32	theme	throughput	608:617	arg1	setting					619:625	a high throughput setting	601:625	a high throughput setting	601:625	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	3	33	theme	Accurate	305:312	arg1	study					314:318	Accurate study	305:318	Accurate study of glycans	305:329	Accurate study of glycans require high resolution mass spectrometry.					
29790907	7	34	theme	network	1043:1049	arg1	regularization					1061:1074	network Laplacian regularization	1043:1074	network Laplacian regularization	1043:1074	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	0	35	theme	glycan	36:41	arg1	profiling					49:57	glycan LC-MS profiling	36:57	glycan LC-MS profiling	36:57	Application of network smoothing to glycan LC-MS profiling.					
29790907	4	36	theme	high	603:606	arg1	setting					619:625	a high throughput setting	601:625	a high throughput setting	601:625	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	2	37	theme	mass	202:205	arg1	LC-MS					221:225	LC-MS	221:225	LC-MS	221:225	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	37	theme	mass	202:205	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	37	theme	mass	202:205	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	38	theme	throughput	245:254	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	38	theme	throughput	245:254	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	6	39	dep	Results	789:795	arg1	developed					800:808	developed	800:808	developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans	800:933	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	2	40	theme	coupled	194:200	arg1	LC-MS					221:225	LC-MS	221:225	LC-MS	221:225	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	40	theme	coupled	194:200	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	40	theme	coupled	194:200	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	41	theme	high	240:243	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	41	theme	high	240:243	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	4	42	theme	Mass	374:377	arg1	spectrometry					379:390	Mass spectrometry	374:390	Mass spectrometry data	374:395	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	43	used	used	514:517	arg2	it					504:505	it	504:505	it	504:505	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	9	44	theme	glycomics	1471:1479	arg1	data					1481:1484	glycomics data	1471:1484	glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph	1471:1618	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	3	45	theme	glycans	323:329	arg1	study					314:318	Accurate study	305:318	Accurate study of glycans	305:329	Accurate study of glycans require high resolution mass spectrometry.					
29790907	2	46	theme	chromatography	179:192	arg1	LC-MS					221:225	LC-MS	221:225	LC-MS	221:225	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	46	theme	chromatography	179:192	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	46	theme	chromatography	179:192	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	47	theme	common	233:238	arg1	spectrometry					207:218	Liquid chromatography coupled mass spectrometry	172:218	Liquid chromatography coupled mass spectrometry (LC-MS)	172:226	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	2	47	theme	common	233:238	arg1	method					256:261	a common high throughput method	231:261	a common high throughput method for analyzing complex biological samples	231:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	1	48	theme	heterogeneous	104:116	arg1	modifications					157:169	the most heterogeneous and complex protein post-translational modifications	95:169	the most heterogeneous and complex protein post-translational modifications	95:169	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	0	49	theme	LC-MS	43:47	arg1	profiling					49:57	glycan LC-MS profiling	36:57	glycan LC-MS profiling	36:57	Application of network smoothing to glycan LC-MS profiling.					
29790907	10	50	dep	https	1726:1730	arg1	//github.com/BostonUniversityCBMS/glycresoft					1732:1775	//github.com/BostonUniversityCBMS/glycresoft	1732:1775	https://github.com/BostonUniversityCBMS/glycresoft	1726:1775	Availability and implementation Built Executable http://www.bumc.bu.edu/msr/glycresoft/ and Source Code: https://github.com/BostonUniversityCBMS/glycresoft.					
29790907	4	51	contain	contains	397:404	arg2	abundance					452:460	abundance	452:460	abundance	452:460	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	51	contain	contains	397:404	arg2	mass					443:446	mass	443:446	mass	443:446	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	51	contain	contains	397:404	arg2	sub-structures					416:429	intricate sub-structures	406:429	intricate sub-structures that encode mass and abundance	406:460	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	4	51	contain	contains	397:404	arg1	data					392:395	Mass spectrometry data	374:395	Mass spectrometry data	374:395	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	7	52	theme	chemical	1014:1021	arg1	properties					1023:1032	glycan chemical properties	1007:1032	glycan chemical properties	1007:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	5	53	theme	individual	736:745	arg1	observations					747:758	individual observations	736:758	individual observations	736:758	Existing tools for interpreting the resulting data do not take into account related glycans when evaluating individual observations, limiting their sensitivity.					
29790907	6	54	theme	LC-MS	862:866	arg1	data					868:871	LC-MS data	862:871	LC-MS data	862:871	Results We developed an algorithm for assigning glycan compositions from LC-MS data by exploring biosynthetic network relationships among glycans.					
29790907	2	55	theme	complex	277:283	arg1	samples					296:302	complex biological samples	277:302	complex biological samples	277:302	Liquid chromatography coupled mass spectrometry (LC-MS) is a common high throughput method for analyzing complex biological samples.					
29790907	4	56	theme	spectrometry	379:390	arg1	data					392:395	Mass spectrometry data	374:395	Mass spectrometry data	374:395	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	1	57	theme	complex	122:128	arg1	modifications					157:169	the most heterogeneous and complex protein post-translational modifications	95:169	the most heterogeneous and complex protein post-translational modifications	95:169	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	9	58	theme	other	1491:1495	arg1	families					1504:1511	other glycan families	1491:1511	other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph	1491:1618	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	7	59	theme	expected	1115:1122	arg1	families					1131:1138	expected glycan families	1115:1138	expected glycan families	1115:1138	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	7	60	theme	glycan	1124:1129	arg1	families					1131:1138	expected glycan families	1115:1138	expected glycan families	1115:1138	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	7	61	theme	prior	1091:1095	arg1	information					1097:1107	prior information	1091:1107	prior information about expected glycan families	1091:1138	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	1	62	theme	protein	130:136	arg1	modifications					157:169	the most heterogeneous and complex protein post-translational modifications	95:169	the most heterogeneous and complex protein post-translational modifications	95:169	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	7	63	theme	scoring	980:986	arg1	functions					988:996	likelihood scoring functions	969:996	likelihood scoring functions based on glycan chemical properties	969:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	9	64	theme	glycan	1497:1502	arg1	families					1504:1511	other glycan families	1491:1511	other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph	1491:1618	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	3	65	theme	mass	355:358	arg1	spectrometry					360:371	high resolution mass spectrometry	339:371	high resolution mass spectrometry	339:371	Accurate study of glycans require high resolution mass spectrometry.					
29790907	4	66	theme	several	473:479	arg1	transformations					481:495	several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting	473:625	several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting	473:625	Mass spectrometry data contains intricate sub-structures that encode mass and abundance, requiring several transformations before it can be used to identify biological molecules, requiring automated tools to analyze samples in a high throughput setting.					
29790907	11	67	theme	Supplementary	1804:1816	arg1	data					1818:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data are available at Bioinformatics online.					
29790907	1	68	theme	post-translational	138:155	arg1	modifications					157:169	the most heterogeneous and complex protein post-translational modifications	95:169	the most heterogeneous and complex protein post-translational modifications	95:169	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	7	69	theme	functions	988:996	arg1	functions					988:996	likelihood scoring functions	969:996	likelihood scoring functions based on glycan chemical properties	969:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	7	69	theme	functions	988:996	arg1	set					962:964	a set	960:964	a set of likelihood scoring functions based on glycan chemical properties	960:1032	Our algorithm optimizes a set of likelihood scoring functions based on glycan chemical properties but uses network Laplacian regularization and optionally prior information about expected glycan families to smooth the likelihood and thus achieve a consistent and more representative solution.					
29790907	9	70	from	families	1504:1511	arg1	data					1481:1484	glycomics data	1471:1484	glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph	1471:1618	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	9	71	theme	network	1393:1399	arg1	definition					1401:1410	Our network definition	1389:1410	Our network definition	1389:1410	Our network definition was tailored to N-glycans but the method may be applied to glycomics data from other glycan families like O-glycans or heparan sulfate where the relationships between compositions can be expressed as a graph.					
29790907	11	72	theme	Supplementary	1778:1790	arg1	data					1818:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data	1778:1821	Supplementary information Supplementary data are available at Bioinformatics online.					
29790907	1	73	theme	modifications	157:169	arg1	modifications					157:169	the most heterogeneous and complex protein post-translational modifications	95:169	the most heterogeneous and complex protein post-translational modifications	95:169	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
29790907	1	73	theme	modifications	157:169	arg1	one					88:90	one	88:90	one	88:90	Motivation Glycosylation is one of the most heterogeneous and complex protein post-translational modifications.					
30714583	3	0	theme	galactosylated	357:370	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	9	1	theme	IgG	1225:1227	arg1	candidates					1275:1284	suitable candidates	1266:1284	suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA	1266:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	1	theme	IgG	1225:1227	arg1	patterns					1236:1243	their different IgG glycan patterns	1209:1243	their different IgG glycan patterns	1209:1243	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	8	2	theme	Similar	1069:1075	arg1	results					1077:1083	Similar results	1069:1083	Similar results	1069:1083	Similar results were found in the validation set.					
30714583	0	3	theme	osteoarthritis	82:95	arg1	patients					97:104	rheumatoid arthritis and osteoarthritis patients	57:104	rheumatoid arthritis and osteoarthritis patients	57:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	9	4	theme	glycan	1229:1234	arg1	candidates					1275:1284	suitable candidates	1266:1284	suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA	1266:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	4	theme	glycan	1229:1234	arg1	patterns					1236:1243	their different IgG glycan patterns	1209:1243	their different IgG glycan patterns	1209:1243	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	5	5	theme	serum	603:607	arg1	IgG					609:611	serum IgG	603:611	serum IgG from RA and OA	603:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	3	6	from	arthritis	409:417	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	2	7	located	found	278:282	arg2	patterns					259:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	2	7	located	found	278:282	arg1	diseases					295:302	various diseases	287:302	various diseases	287:302	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	3	8	theme	rheumatoid	398:407	arg1	RA					420:421	RA	420:421	RA	420:421	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	8	theme	rheumatoid	398:407	arg1	arthritis					409:417	rheumatoid arthritis	398:417	rheumatoid arthritis (RA)	398:422	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	0	9	from	Distribution	0:11	arg1	patients					97:104	rheumatoid arthritis and osteoarthritis patients	57:104	rheumatoid arthritis and osteoarthritis patients	57:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	7	10	theme	selected	956:963	arg1	glycans					965:971	the selected glycans	952:971	the selected glycans	952:971	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	5	11	theme	IgG	609:611	arg1	forms					594:598	15 glycan forms	584:598	15 glycan forms of serum IgG from RA and OA	584:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	8	12	theme	validation	1103:1112	arg1	set					1114:1116	the validation set	1099:1116	the validation set	1099:1116	Similar results were found in the validation set.					
30714583	8	13	located	found	1090:1094	arg2	results					1077:1083	Similar results	1069:1083	Similar results	1069:1083	Similar results were found in the validation set.					
30714583	8	13	located	found	1090:1094	arg1	set					1114:1116	the validation set	1099:1116	the validation set	1099:1116	Similar results were found in the validation set.					
30714583	3	14	theme	IgG	391:393	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	7	15	theme	RF/ACPA	1048:1054	arg1	RA					1065:1066	RF/ACPA negative RA	1048:1066	RF/ACPA negative RA	1048:1066	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	2	16	theme	healthy	316:322	arg1	controls					324:331	healthy controls	316:331	healthy controls	316:331	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	7	17	theme	negative	1056:1063	arg1	RA					1065:1066	RF/ACPA negative RA	1048:1066	RF/ACPA negative RA	1048:1066	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	7	18	theme	Combined	927:934	arg1	analysis					940:947	Combined ROC analysis	927:947	Combined ROC analysis of the selected glycans	927:971	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	5	19	theme	healthy	743:749	arg1	controls					751:758	healthy controls	743:758	healthy controls (AUC > 0.9)	743:770	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	5	19	theme	healthy	743:749	arg1	>					765:765	AUC > 0.9	761:769	AUC > 0.9	761:769	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	4	20	theme	glycan	506:511	arg1	patterns					513:520	glycan patterns	506:520	glycan patterns	506:520	However, how glycan patterns differ between RA and OA remains unclear.					
30714583	5	21	theme	glycan	587:592	arg1	forms					594:598	15 glycan forms	584:598	15 glycan forms of serum IgG from RA and OA	584:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	0	22	theme	IgG	25:27	arg1	patterns					43:50	abnormal IgG glycosylation patterns	16:50	abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients	16:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	9	23	theme	suitable	1266:1273	arg1	candidates					1275:1284	suitable candidates	1266:1284	suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA	1266:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	23	theme	suitable	1266:1273	arg1	patterns					1236:1243	their different IgG glycan patterns	1209:1243	their different IgG glycan patterns	1209:1243	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	5	24	theme	suitable	696:703	arg1	candidate					705:713	a suitable candidate	694:713	a suitable candidate for differentiating RA from healthy controls (AUC > 0.9)	694:770	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	6	25	theme	binary	792:797	arg1	regression					808:817	binary logistic regression	792:817	binary logistic regression	792:817	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	6	26	theme	logistic	799:806	arg1	regression					808:817	binary logistic regression	792:817	binary logistic regression	792:817	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	1	27	theme	proteins	231:238	arg1	function					219:226	function	219:226	function	219:226	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	1	27	theme	proteins	231:238	arg1	structure					205:213	structure	205:213	structure	205:213	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	0	28	theme	abnormal	16:23	arg1	patterns					43:50	abnormal IgG glycosylation patterns	16:50	abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients	16:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	0	29	from	patients	97:104	arg1	patterns					43:50	abnormal IgG glycosylation patterns	16:50	abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients	16:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	0	29	from	patients	97:104	arg1	Distribution					0:11	Distribution	0:11	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn	0:121	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	9	30	theme	different	1215:1223	arg1	candidates					1275:1284	suitable candidates	1266:1284	suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA	1266:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	30	theme	different	1215:1223	arg1	patterns					1236:1243	their different IgG glycan patterns	1209:1243	their different IgG glycan patterns	1209:1243	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	0	31	theme	patterns	43:50	arg1	Distribution					0:11	Distribution	0:11	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn	0:121	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	5	32	from	OA	625:626	arg1	forms					594:598	15 glycan forms	584:598	15 glycan forms of serum IgG from RA and OA	584:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	5	32	from	OA	625:626	arg1	IgG					609:611	serum IgG	603:611	serum IgG from RA and OA	603:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	6	33	theme	N-acetylglucosamine	849:867	arg1	glycoforms					878:887	three bisecting N-acetylglucosamine (GlcNAc) glycoforms	833:887	three bisecting N-acetylglucosamine (GlcNAc) glycoforms	833:887	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	0	34	theme	glycosylation	29:41	arg1	patterns					43:50	abnormal IgG glycosylation patterns	16:50	abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients	16:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	6	35	theme	bisecting	839:847	arg1	GlcNAc					870:875	GlcNAc	870:875	GlcNAc	870:875	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	6	35	theme	bisecting	839:847	arg1	N-acetylglucosamine					849:867	bisecting N-acetylglucosamine	839:867	three bisecting N-acetylglucosamine (GlcNAc) glycoforms	833:887	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	0	36	theme	rheumatoid	57:66	arg1	arthritis					68:76	rheumatoid arthritis	57:76	rheumatoid arthritis	57:76	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	7	37	theme	0.79	1028:1031	arg1	AUC					984:986	an AUC	981:986	an AUC of 0.81 between OA and RA	981:1012	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	7	37	theme	0.79	1028:1031	arg1	AUC					1021:1023	an AUC	1018:1023	an AUC of 0.79 between OA and RF/ACPA negative RA	1018:1066	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	0	38	theme	arthritis	68:76	arg1	patients					97:104	rheumatoid arthritis and osteoarthritis patients	57:104	rheumatoid arthritis and osteoarthritis patients	57:104	Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn.					
30714583	1	39	dep	structure	205:213	arg1	the					201:203	the	201:203	the	201:203	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	5	40	from	forms	594:598	arg1	OA					625:626	OA	625:626	OA	625:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	5	40	from	forms	594:598	arg1	RA					618:619	RA	618:619	RA	618:619	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	9	41	theme	patterns	1236:1243	arg1	basis					1200:1204	the basis	1196:1204	the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA	1196:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	3	42	theme	terminal	348:355	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	2	43	theme	N-glycan	250:257	arg1	patterns					259:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	3	44	from	N-glycans	372:380	arg1	OA					444:445	OA	444:445	OA	444:445	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	44	from	N-glycans	372:380	arg1	RA					420:421	RA	420:421	RA	420:421	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	44	from	N-glycans	372:380	arg1	osteoarthritis					428:441	osteoarthritis	428:441	osteoarthritis (OA)	428:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	44	from	N-glycans	372:380	arg1	arthritis					409:417	rheumatoid arthritis	398:417	rheumatoid arthritis (RA)	398:422	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	6	45	gly	glycoforms	878:887	arg1	GlcNAc					870:875	GlcNAc	870:875	GlcNAc	870:875	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	6	45	gly	glycoforms	878:887	arg1	N-acetylglucosamine					849:867	bisecting N-acetylglucosamine	839:867	three bisecting N-acetylglucosamine (GlcNAc) glycoforms	833:887	Then, we performed binary logistic regression to screen out three bisecting N-acetylglucosamine (GlcNAc) glycoforms for distinguishing between OA and RA.					
30714583	3	46	from	IgG	391:393	arg1	OA					444:445	OA	444:445	OA	444:445	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	46	from	IgG	391:393	arg1	RA					420:421	RA	420:421	RA	420:421	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	46	from	IgG	391:393	arg1	osteoarthritis					428:441	osteoarthritis	428:441	osteoarthritis (OA)	428:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	46	from	IgG	391:393	arg1	arthritis					409:417	rheumatoid arthritis	398:417	rheumatoid arthritis (RA)	398:422	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	2	47	theme	Abnormal	241:248	arg1	patterns					259:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns	241:266	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	5	48	theme	AUC	761:763	arg1	controls					751:758	healthy controls	743:758	healthy controls (AUC > 0.9)	743:770	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	5	48	theme	AUC	761:763	arg1	>					765:765	AUC > 0.9	761:769	AUC > 0.9	761:769	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	1	49	theme	post-translational	143:160	arg1	modification					162:173	a post-translational modification	141:173	a post-translational modification essential for maintaining the structure and function of proteins	141:238	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	1	49	theme	post-translational	143:160	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	9	50	theme	clinical	1325:1332	arg1	OA					1360:1361	OA	1360:1361	OA	1360:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	50	theme	clinical	1325:1332	arg1	conditions					1334:1343	clinical conditions	1325:1343	clinical conditions such as RA and OA	1325:1361	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	9	50	theme	clinical	1325:1332	arg1	RA					1353:1354	RA	1353:1354	RA	1353:1354	In conclusion, our analysis demonstrates that RA and OA are distinguished on the basis of their different IgG glycan patterns, which thus serve as suitable candidates as biomarkers for reliably identifying clinical conditions such as RA and OA.					
30714583	7	51	theme	0.81	991:994	arg1	AUC					984:986	an AUC	981:986	an AUC of 0.81 between OA and RA	981:1012	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	7	51	theme	0.81	991:994	arg1	AUC					1021:1023	an AUC	1018:1023	an AUC of 0.79 between OA and RF/ACPA negative RA	1018:1066	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	7	52	theme	ROC	936:938	arg1	analysis					940:947	Combined ROC analysis	927:947	Combined ROC analysis of the selected glycans	927:971	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	3	53	from	decrease	336:343	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	2	54	theme	various	287:293	arg1	diseases					295:302	various diseases	287:302	various diseases	287:302	Abnormal N-glycan patterns have been found in various diseases compared to healthy controls.					
30714583	1	55	theme	essential	175:183	arg1	modification					162:173	a post-translational modification	141:173	a post-translational modification essential for maintaining the structure and function of proteins	141:238	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	1	55	theme	essential	175:183	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation is a post-translational modification essential for maintaining the structure and function of proteins.					
30714583	3	56	theme	serum	385:389	arg1	IgG					391:393	serum IgG	385:393	serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	385:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	3	57	from	osteoarthritis	428:441	arg1	N-glycans					372:380	terminal galactosylated N-glycans	348:380	terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA)	348:446	A decrease in terminal galactosylated N-glycans of serum IgG in rheumatoid arthritis (RA) and osteoarthritis (OA) may be involved in their immunopathogenesis.					
30714583	7	58	theme	glycans	965:971	arg1	analysis					940:947	Combined ROC analysis	927:947	Combined ROC analysis of the selected glycans	927:971	Combined ROC analysis of the selected glycans yielded an AUC of 0.81 between OA and RA and an AUC of 0.79 between OA and RF/ACPA negative RA.					
30714583	5	59	from	RA	618:619	arg1	forms					594:598	15 glycan forms	584:598	15 glycan forms of serum IgG from RA and OA	584:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
30714583	5	59	from	RA	618:619	arg1	IgG					609:611	serum IgG	603:611	serum IgG from RA and OA	603:626	Here, we identified 15 glycan forms of serum IgG from RA and OA using MALDI-TOF MS. We found that IgG galactosylation represented a suitable candidate for differentiating RA from healthy controls (AUC > 0.9).					
31569500	2	0	gly	O-mannosylation	298:312	arg1	proteins					338:345	proteins	338:345	proteins	338:345	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	6	1	theme	luminal	977:983	arg1	loops					985:989	luminal loops	977:989	luminal loops	977:989	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	3	2	theme	oligosaccharyltransferases	506:531	arg1	structures					547:556	oligosaccharyltransferases (STT3 family) structures	506:556	oligosaccharyltransferases (STT3 family) structures	506:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	1	3	link	polyisoprenol-linked	106:125	arg1	substrates					133:142	polyisoprenol-linked donor substrates	106:142	polyisoprenol-linked donor substrates	106:142	Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily.					
31569500	7	4	dep	11	1096:1097	arg1	PIGZ					1141:1144	PIGZ	1141:1144	PIGZ	1141:1144	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	POMT					1100:1103	POMT	1100:1103	POMT	1100:1103	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	ALG12					1118:1122	ALG12	1118:1122	ALG12	1118:1122	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	PIGB					1125:1128	PIGB	1125:1128	PIGB	1125:1128	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	TMTC					1106:1109	TMTC	1106:1109	TMTC	1106:1109	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	ALG9					1112:1115	ALG9	1112:1115	ALG9	1112:1115	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	4	dep	11	1096:1097	arg1	PIGV					1131:1134	PIGV	1131:1134	PIGV	1131:1134	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	4	5	theme	members	590:596	arg1	alignment					570:578	A multiple alignment	559:578	A multiple alignment of family members from diverse organisms	559:619	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	3	6	theme	membrane	363:370	arg1	model					381:385	a membrane topology model	361:385	a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures	361:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	5	7	from	positioned	785:794	arg1	loops					807:811	luminal loops	799:811	luminal loops that showed high conservation within the DPY19 family	799:865	Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family.					
31569500	7	8	theme	11	1096:1097	arg1	helices					1196:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	9	9	theme	evolutionary	1405:1416	arg1	enzymes					1426:1432	evolutionary related enzymes	1405:1432	evolutionary related enzymes with preserved membrane topology	1405:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	9	9	theme	evolutionary	1405:1416	arg1	members					1369:1375	all members	1365:1375	all members of the GT-C superfamily	1365:1399	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	3	10	theme	topology	372:379	arg1	model					381:385	a membrane topology model	361:385	a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures	361:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	6	11	theme	GT-C	908:911	arg1	families					913:920	other eukaryotic GT-C families	891:920	other eukaryotic GT-C families	891:920	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	7	12	theme	Most	1027:1030	arg1	enzymes					1037:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	9	13	theme	superfamily	1389:1399	arg1	members					1369:1375	all members	1365:1375	all members of the GT-C superfamily	1365:1399	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	9	13	theme	superfamily	1389:1399	arg1	enzymes					1426:1432	evolutionary related enzymes	1405:1432	evolutionary related enzymes with preserved membrane topology	1405:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	4	14	theme	diverse	603:609	arg1	organisms					611:619	diverse organisms	603:619	diverse organisms	603:619	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	2	15	gly	N-glycosylation	269:283	arg1	proteins					338:345	proteins	338:345	proteins	338:345	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	6	16	theme	eukaryotic	897:906	arg1	families					913:920	other eukaryotic GT-C families	891:920	other eukaryotic GT-C families	891:920	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	7	17	theme	TDMs	1086:1089	arg1	number					1076:1081	an uneven number	1066:1081	an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1066:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	4	18	theme	acids	676:680	arg1	presence					637:644	the presence	633:644	the presence of only a few conserved amino acids between DPY19s and STT3s	633:705	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	5	19	from	loops	807:811	arg1	positioned					785:794	positioned	785:794	positioned	785:794	Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family.					
31569500	4	20	theme	amino	670:674	arg1	acids					676:680	only a few conserved amino acids	649:680	only a few conserved amino acids	649:680	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	0	21	theme	Topological	9:19	arg1	Model					21:25	Membrane Topological Model	0:25	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.	0:74	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.					
31569500	8	22	contain	have	1245:1248	arg1	PIGM					1218:1221	PIGM	1218:1221	PIGM	1218:1221	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	8	22	contain	have	1245:1248	arg1	ALG8					1240:1243	ALG8	1240:1243	ALG8	1240:1243	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	8	22	contain	have	1245:1248	arg1	ALG6					1230:1233	ALG6	1230:1233	ALG6	1230:1233	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	8	22	contain	have	1245:1248	arg2	TMDs					1259:1262	12 or 14 TMDs	1250:1262	12 or 14 TMDs	1250:1262	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	8	22	contain	have	1245:1248	arg1	ALG3					1224:1227	ALG3	1224:1227	ALG3	1224:1227	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	9	23	theme	preserved	1439:1447	arg1	topology					1458:1465	preserved membrane topology	1439:1465	preserved membrane topology	1439:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	0	24	theme	Membrane	0:7	arg1	Model					21:25	Membrane Topological Model	0:25	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.	0:74	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.					
31569500	6	25	from	enzymes	999:1005	arg1	presence					937:944	the presence	933:944	the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily	933:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	1	26	theme	polyisoprenol-linked	106:125	arg1	substrates					133:142	polyisoprenol-linked donor substrates	106:142	polyisoprenol-linked donor substrates	106:142	Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily.					
31569500	7	27	contain	have	1061:1064	arg2	number					1076:1081	an uneven number	1066:1081	an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1066:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	27	contain	have	1061:1064	arg1	enzymes					1037:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	9	28	theme	related	1418:1424	arg1	enzymes					1426:1432	evolutionary related enzymes	1405:1432	evolutionary related enzymes with preserved membrane topology	1405:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	9	28	theme	related	1418:1424	arg1	members					1369:1375	all members	1365:1375	all members of the GT-C superfamily	1365:1399	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	4	29	theme	multiple	561:568	arg1	alignment					570:578	A multiple alignment	559:578	A multiple alignment of family members from diverse organisms	559:619	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	1	30	theme	donor	127:131	arg1	substrates					133:142	polyisoprenol-linked donor substrates	106:142	polyisoprenol-linked donor substrates	106:142	Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily.					
31569500	6	31	theme	families	913:920	arg1	alignments					877:886	Multiple alignments	868:886	Multiple alignments of other eukaryotic GT-C families	868:920	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	5	32	theme	high	825:828	arg1	conservation					830:841	high conservation	825:841	high conservation within the DPY19 family	825:865	Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family.					
31569500	4	33	theme	family	583:588	arg1	members					590:596	family members	583:596	family members	583:596	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	7	34	theme	uneven	1069:1074	arg1	number					1076:1081	an uneven number	1066:1081	an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1066:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	9	35	theme	membrane	1449:1456	arg1	topology					1458:1465	preserved membrane topology	1439:1465	preserved membrane topology	1439:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	1	36	theme	GT-C	167:170	arg1	superfamily					172:182	the GT-C superfamily	163:182	the GT-C superfamily	163:182	Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily.					
31569500	9	37	with	enzymes	1426:1432	arg1	topology					1458:1465	preserved membrane topology	1439:1465	preserved membrane topology	1439:1465	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	0	38	theme	Superfamily	63:73	arg1	Glycosyltransferases					30:49	Glycosyltransferases	30:49	Glycosyltransferases of the GT-C Superfamily	30:73	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.					
31569500	7	39	dep	13	1150:1151	arg1	STT3					1161:1164	STT3	1161:1164	STT3	1161:1164	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	39	dep	13	1150:1151	arg1	ALG10					1171:1175	ALG10	1171:1175	ALG10	1171:1175	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	39	dep	13	1150:1151	arg1	DPY19					1154:1158	DPY19	1154:1158	DPY19	1154:1158	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	6	40	theme	Multiple	868:875	arg1	alignments					877:886	Multiple alignments	868:886	Multiple alignments of other eukaryotic GT-C families	868:920	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	4	41	theme	conserved	660:668	arg1	acids					676:680	only a few conserved amino acids	649:680	only a few conserved amino acids	649:680	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	0	42	theme	Glycosyltransferases	30:49	arg1	Model					21:25	Membrane Topological Model	0:25	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.	0:74	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.					
31569500	6	43	theme	motifs	967:972	arg1	presence					937:944	the presence	933:944	the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily	933:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	6	44	from	loops	985:989	arg1	presence					937:944	the presence	933:944	the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily	933:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	3	45	theme	transmembrane	465:477	arg1	TMDs					488:491	TMDs	488:491	TMDs	488:491	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	45	theme	transmembrane	465:477	arg1	domains					479:485	the 13 transmembrane domains	458:485	the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures	458:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	46	theme	DPY19	414:418	arg1	C-mannosyltransferases					390:411	C-mannosyltransferases	390:411	C-mannosyltransferases (DPY19 family)	390:426	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	46	theme	DPY19	414:418	arg1	family					420:425	DPY19 family	414:425	DPY19 family	414:425	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	4	47	from	organisms	611:619	arg1	alignment					570:578	A multiple alignment	559:578	A multiple alignment of family members from diverse organisms	559:619	A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s.					
31569500	2	48	gly	C-mannosylation	319:333	arg1	proteins					338:345	proteins	338:345	proteins	338:345	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	6	49	theme	other	891:895	arg1	families					913:920	other eukaryotic GT-C families	891:920	other eukaryotic GT-C families	891:920	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	5	50	theme	luminal	799:805	arg1	loops					807:811	luminal loops	799:811	luminal loops that showed high conservation within the DPY19 family	799:865	Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family.					
31569500	6	51	theme	similar	949:955	arg1	motifs					967:972	similar conserved motifs	949:972	similar conserved motifs	949:972	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	2	52	theme	reticulum	228:236	arg1	lumen					243:247	the endoplasmic reticulum (ER) lumen	212:247	the endoplasmic reticulum (ER) lumen	212:247	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	7	53	theme	GT-C	1032:1035	arg1	enzymes					1037:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes	1027:1043	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	54	theme	membrane-spanning	1178:1194	arg1	helices					1196:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	6	55	from	motifs	967:972	arg1	enzymes					999:1005	all enzymes	995:1005	all enzymes of the superfamily	995:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	6	55	from	motifs	967:972	arg1	loops					985:989	luminal loops	977:989	luminal loops	977:989	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	2	56	theme	endoplasmic	216:226	arg1	ER					239:240	ER	239:240	ER	239:240	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	2	56	theme	endoplasmic	216:226	arg1	reticulum					228:236	endoplasmic reticulum	216:236	the endoplasmic reticulum (ER) lumen	212:247	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	8	57	theme	C-terminal	1278:1287	arg1	motif					1311:1315	a C-terminal dilysine ER-retrieval motif	1276:1315	a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm	1276:1346	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	2	58	theme	proteins	338:345	arg1	glypiation					286:295	glypiation	286:295	glypiation	286:295	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	2	58	theme	proteins	338:345	arg1	N-glycosylation					269:283	N-glycosylation	269:283	N-glycosylation	269:283	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	2	58	theme	proteins	338:345	arg1	O-mannosylation					298:312	O-mannosylation	298:312	O-mannosylation	298:312	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	2	58	theme	proteins	338:345	arg1	C-mannosylation					319:333	C-mannosylation	319:333	C-mannosylation	319:333	In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins.					
31569500	9	59	theme	GT-C	1384:1387	arg1	superfamily					1389:1399	the GT-C superfamily	1380:1399	the GT-C superfamily	1380:1399	We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.					
31569500	7	60	with	TDMs	1086:1089	arg1	helices					1196:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	7	61	theme	13	1150:1151	arg1	helices					1196:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices	1096:1202	Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices.					
31569500	5	62	theme	DPY19	854:858	arg1	family					860:865	the DPY19 family	850:865	the DPY19 family	850:865	Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family.					
31569500	8	63	theme	ER-retrieval	1298:1309	arg1	motif					1311:1315	a C-terminal dilysine ER-retrieval motif	1276:1315	a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm	1276:1346	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	3	64	located	observed	494:501	arg2	TMDs					488:491	TMDs	488:491	TMDs	488:491	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	64	located	observed	494:501	arg2	domains					479:485	the 13 transmembrane domains	458:485	the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures	458:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	64	located	observed	494:501	arg1	structures					547:556	oligosaccharyltransferases (STT3 family) structures	506:556	oligosaccharyltransferases (STT3 family) structures	506:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	65	theme	STT3	534:537	arg1	structures					547:556	oligosaccharyltransferases (STT3 family) structures	506:556	oligosaccharyltransferases (STT3 family) structures	506:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	3	66	theme	C-mannosyltransferases	390:411	arg1	model					381:385	a membrane topology model	361:385	a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures	361:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	6	67	theme	superfamily	1014:1024	arg1	enzymes					999:1005	all enzymes	995:1005	all enzymes of the superfamily	995:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	0	68	theme	GT-C	58:61	arg1	Superfamily					63:73	the GT-C Superfamily	54:73	the GT-C Superfamily	54:73	Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily.					
31569500	8	69	theme	dilysine	1289:1296	arg1	motif					1311:1315	a C-terminal dilysine ER-retrieval motif	1276:1315	a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm	1276:1346	In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm.					
31569500	3	70	theme	family	539:544	arg1	structures					547:556	oligosaccharyltransferases (STT3 family) structures	506:556	oligosaccharyltransferases (STT3 family) structures	506:556	We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures.					
31569500	6	71	from	presence	937:944	arg1	enzymes					999:1005	all enzymes	995:1005	all enzymes of the superfamily	995:1024	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	6	71	from	presence	937:944	arg1	loops					985:989	luminal loops	977:989	luminal loops	977:989	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
31569500	6	72	theme	conserved	957:965	arg1	motifs					967:972	similar conserved motifs	949:972	similar conserved motifs	949:972	Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily.					
29726048	0	0	theme	dynamics	76:83	arg1	simulation					85:94	molecular dynamics simulation	66:94	molecular dynamics simulation	66:94	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	2	1	from	function	424:431	arg1	structure					354:362	the glycosylation structure	336:362	the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP	336:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	8	2	theme	surrounding	1441:1451	arg1	residues					1453:1460	its surrounding residues	1437:1460	its surrounding residues	1437:1460	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	0	3	theme	molecular	66:74	arg1	simulation					85:94	molecular dynamics simulation	66:94	molecular dynamics simulation	66:94	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	2	4	dep	structure	410:418	arg1	the					406:408	the	406:408	the	406:408	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	0	5	from	Role	0:3	arg1	protein					46:52	cholesteryl ester transfer protein	19:52	cholesteryl ester transfer protein	19:52	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	6	6	theme	Helix-X	1136:1142	arg1	flexibility					1121:1131	the flexibility	1117:1131	the flexibility of Helix-X to interfere the CEs transfer	1117:1172	Besides, Glycan240 reduces the flexibility of Helix-X to interfere the CEs transfer.					
29726048	3	7	theme	molecular	474:482	arg1	MD					494:495	MD	494:495	MD	494:495	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	3	7	theme	molecular	474:482	arg1	dynamics					484:491	3.0-μs molecular dynamics	467:491	3.0-μs molecular dynamics (MD)	467:496	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	3	8	theme	CETP-mediated	629:641	arg1	exchange					649:656	the CETP-mediated lipid exchange	625:656	the CETP-mediated lipid exchange	625:656	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	9	9	theme	CVD	1563:1565	arg1	treatment					1550:1558	treatment	1550:1558	treatment	1550:1558	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	9	theme	CVD	1563:1565	arg1	inhibition					1535:1544	CETP inhibition	1530:1544	CETP inhibition	1530:1544	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	3	10	theme	dynamics	484:491	arg1	total					458:462	a total	456:462	a total of 3.0-μs molecular dynamics (MD)	456:496	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	4	11	theme	oscillating	875:885	arg1	area					849:852	solvent-accessible surface area	822:852	higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude	787:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	11	theme	oscillating	875:885	arg1	SASA					855:858	SASA	855:858	SASA	855:858	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	2	12	from	structure	410:418	arg1	structure					354:362	the glycosylation structure	336:362	the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP	336:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	9	13	dep	part	1493:1496	arg1	consider					1580:1587	consider	1580:1587	should fully consider the effect of glycans	1567:1609	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	7	14	theme	hydrophobic	1229:1239	arg1	SASA					1241:1244	the hydrophobic SASA	1225:1244	the hydrophobic SASA of C-terminal	1225:1258	Glycan396 decreases the flexibility and increases the hydrophobic SASA of C-terminal.					
29726048	7	15	theme	C-terminal	1249:1258	arg1	SASA					1241:1244	the hydrophobic SASA	1225:1244	the hydrophobic SASA of C-terminal	1225:1258	Glycan396 decreases the flexibility and increases the hydrophobic SASA of C-terminal.					
29726048	4	16	theme	N-terminal	864:873	arg1	oscillating					875:885	N-terminal oscillating	864:885	N-terminal oscillating with larger amplitude	864:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	5	17	theme	flap	1030:1033	arg1	flexibility					1010:1020	the flexibility	1006:1020	the flexibility of neck flap	1006:1033	Glycan341 reduces the flexibility of neck flap, with the interference of CEs through the neck region.					
29726048	1	18	gly	unglycosylated	184:197	arg1	structure					207:215	the unglycosylated crystal structure	180:215	the unglycosylated crystal structure	180:215	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	9	19	dep	inhibition	1535:1544	arg1	the					1526:1528	the	1526:1528	the	1526:1528	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	3	20	theme	3.0-μs	467:472	arg1	MD					494:495	MD	494:495	MD	494:495	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	3	20	theme	3.0-μs	467:472	arg1	dynamics					484:491	3.0-μs molecular dynamics	467:491	3.0-μs molecular dynamics (MD)	467:496	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	5	21	theme	neck	1025:1028	arg1	flap					1030:1033	neck flap	1025:1033	neck flap	1025:1033	Glycan341 reduces the flexibility of neck flap, with the interference of CEs through the neck region.					
29726048	2	22	theme	CETP	367:370	arg1	structure					354:362	the glycosylation structure	336:362	the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP	336:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	23	theme	CETP	436:439	arg1	function					424:431	function	424:431	function	424:431	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	23	theme	CETP	436:439	arg1	structure					410:418	structure	410:418	structure	410:418	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	6	24	theme	CEs	1161:1163	arg1	transfer					1165:1172	the CEs transfer	1157:1172	the CEs transfer	1157:1172	Besides, Glycan240 reduces the flexibility of Helix-X to interfere the CEs transfer.					
29726048	3	25	theme	lipid	643:647	arg1	exchange					649:656	the CETP-mediated lipid exchange	625:656	the CETP-mediated lipid exchange	625:656	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	4	26	theme	partial	937:943	arg1	assistance					945:954	partial assistance	937:954	partial assistance for CEs	937:962	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	1	27	theme	cardiovascular	255:268	arg1	CVD					279:281	CVD	279:281	CVD	279:281	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	1	27	theme	cardiovascular	255:268	arg1	disease					270:276	cardiovascular disease	255:276	cardiovascular disease (CVD)	255:282	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	9	28	theme	integral	1484:1491	arg1	glycans					1469:1475	glycans	1469:1475	glycans	1469:1475	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	28	theme	integral	1484:1491	arg1	part					1493:1496	an integral part	1481:1496	an integral part of CETP	1481:1504	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	4	29	theme	stretched	766:774	arg1	shape					776:780	a more stretched shape	759:780	a more stretched shape	759:780	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	2	30	theme	glycans	395:401	arg1	CETP					367:370	CETP	367:370	CETP (CETP-G)	367:379	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	30	theme	glycans	395:401	arg1	CETP-G					373:378	CETP-G	373:378	CETP-G	373:378	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	30	theme	glycans	395:401	arg1	effect					385:390	effect	385:390	effect of glycans on the structure and function of CETP	385:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	8	31	theme	surrounding	1355:1365	arg1	residues					1367:1374	surrounding residues	1355:1374	surrounding residues	1355:1374	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	8	32	theme	CETP	1321:1324	arg1	structure					1308:1316	structure	1308:1316	structure	1308:1316	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	8	32	theme	CETP	1321:1324	arg1	dynamics					1295:1302	dynamics	1295:1302	dynamics	1295:1302	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	1	33	theme	Current	97:103	arg1	inhibitors					147:156	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	9	34	theme	CETP	1501:1504	arg1	glycans					1469:1475	glycans	1469:1475	glycans	1469:1475	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	34	theme	CETP	1501:1504	arg1	part					1493:1496	an integral part	1481:1496	an integral part of CETP	1481:1504	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	2	35	theme	effect	385:390	arg1	structure					354:362	the glycosylation structure	336:362	the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP	336:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	0	36	theme	glycans	8:14	arg1	Role					0:3	Role	0:3	Role of glycans in cholesteryl ester transfer protein	0:52	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	4	37	theme	surface	841:847	arg1	area					849:852	solvent-accessible surface area	822:852	higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude	787:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	37	theme	surface	841:847	arg1	SASA					855:858	SASA	855:858	SASA	855:858	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	1	38	theme	cholesteryl	105:115	arg1	protein					132:138	cholesteryl ester transfer protein	105:138	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	1	38	theme	cholesteryl	105:115	arg1	CETP					141:144	CETP	141:144	CETP	141:144	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	1	39	theme	crystal	199:205	arg1	structure					207:215	the unglycosylated crystal structure	180:215	the unglycosylated crystal structure	180:215	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	0	40	theme	ester	31:35	arg1	protein					46:52	cholesteryl ester transfer protein	19:52	cholesteryl ester transfer protein	19:52	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	9	41	theme	glycans	1603:1609	arg1	effect					1593:1598	the effect	1589:1598	the effect of glycans	1589:1609	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	3	42	theme	structure	522:530	arg1	trajectories					498:509	trajectories	498:509	trajectories of nascent structure of CETP (CETP-N) and CETP-G	498:558	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	0	43	theme	cholesteryl	19:29	arg1	protein					46:52	cholesteryl ester transfer protein	19:52	cholesteryl ester transfer protein	19:52	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	5	44	theme	CEs	1061:1063	arg1	interference					1045:1056	the interference	1041:1056	the interference of CEs	1041:1063	Glycan341 reduces the flexibility of neck flap, with the interference of CEs through the neck region.					
29726048	4	45	dep	adopts	752:757	arg1	provides					928:935	provides	928:935	provides partial assistance for CEs through the N-terminal	928:985	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	9	46	theme	CETP	1530:1533	arg1	inhibition					1535:1544	CETP inhibition	1530:1544	CETP inhibition	1530:1544	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	47	theme	further	1507:1513	arg1	studies					1515:1521	further studies	1507:1521	further studies on the CETP inhibition and treatment of CVD	1507:1565	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	48	from	studies	1515:1521	arg1	treatment					1550:1558	treatment	1550:1558	treatment	1550:1558	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	9	48	from	studies	1515:1521	arg1	inhibition					1535:1544	CETP inhibition	1530:1544	CETP inhibition	1530:1544	Thus, glycans are an integral part of CETP, further studies on the CETP inhibition and treatment of CVD should fully consider the effect of glycans.					
29726048	3	49	theme	CETP-G	553:558	arg1	structure					522:530	nascent structure	514:530	nascent structure of CETP (CETP-N) and CETP-G	514:558	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	1	50	dep	designed	162:169	arg1	based					171:175	based	171:175	based on the unglycosylated crystal structure	171:215	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	2	51	from	structure	354:362	arg1	function					424:431	function	424:431	function	424:431	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	51	from	structure	354:362	arg1	structure					410:418	structure	410:418	structure	410:418	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	1	52	theme	ester	117:121	arg1	protein					132:138	cholesteryl ester transfer protein	105:138	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	1	52	theme	ester	117:121	arg1	CETP					141:144	CETP	141:144	CETP	141:144	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	0	53	theme	transfer	37:44	arg1	protein					46:52	cholesteryl ester transfer protein	19:52	cholesteryl ester transfer protein	19:52	Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation.					
29726048	4	54	dep	hydrophobic	794:804	arg1	higher					787:792	higher	787:792	higher	787:792	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	55	theme	hydrophobic	794:804	arg1	area					849:852	solvent-accessible surface area	822:852	higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude	787:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	55	theme	hydrophobic	794:804	arg1	SASA					855:858	SASA	855:858	SASA	855:858	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	3	56	theme	nascent	514:520	arg1	structure					522:530	nascent structure	514:530	nascent structure of CETP (CETP-N) and CETP-G	514:558	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	1	57	theme	transfer	123:130	arg1	protein					132:138	cholesteryl ester transfer protein	105:138	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	1	57	theme	transfer	123:130	arg1	CETP					141:144	CETP	141:144	CETP	141:144	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	5	58	theme	neck	1077:1080	arg1	region					1082:1087	the neck region	1073:1087	the neck region	1073:1087	Glycan341 reduces the flexibility of neck flap, with the interference of CEs through the neck region.					
29726048	4	59	theme	solvent-accessible	822:839	arg1	area					849:852	solvent-accessible surface area	822:852	higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude	787:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	59	theme	solvent-accessible	822:839	arg1	SASA					855:858	SASA	855:858	SASA	855:858	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	60	theme	mutation	707:714	arg1	studies					716:722	previous mutation studies	698:722	previous mutation studies	698:722	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	3	61	used	used	451:454	arg2	we					448:449	we	448:449	we	448:449	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	2	62	from	CETP	367:370	arg1	function					424:431	function	424:431	function	424:431	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	62	from	CETP	367:370	arg1	structure					410:418	structure	410:418	structure	410:418	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	1	63	theme	protein	132:138	arg1	inhibitors					147:156	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors	97:156	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	4	64	theme	hydrophilic	810:820	arg1	area					849:852	solvent-accessible surface area	822:852	higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude	787:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	64	theme	hydrophilic	810:820	arg1	SASA					855:858	SASA	855:858	SASA	855:858	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	8	65	theme	glycan	1410:1415	arg1	conformations					1393:1405	the sampled conformations	1381:1405	the sampled conformations of glycan	1381:1415	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	4	66	theme	previous	698:705	arg1	studies					716:722	previous mutation studies	698:722	previous mutation studies	698:722	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	4	67	with	oscillating	875:885	arg1	amplitude					899:907	larger amplitude	892:907	larger amplitude	892:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	8	68	theme	sampled	1385:1391	arg1	conformations					1393:1405	the sampled conformations	1381:1405	the sampled conformations of glycan	1381:1415	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	4	69	theme	larger	892:897	arg1	amplitude					899:907	larger amplitude	892:907	larger amplitude	892:907	In accordance with our simulations and previous mutation studies, relative to CETP-N, CETP-G adopts a more stretched shape with higher hydrophobic and hydrophilic solvent-accessible surface area (SASA) of N-terminal oscillating with larger amplitude, in which Glycan88 provides partial assistance for CEs through the N-terminal.					
29726048	3	70	theme	structural	575:584	arg1	differentiations					586:601	their structural differentiations	569:601	their structural differentiations	569:601	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	3	71	theme	CETP	535:538	arg1	structure					522:530	nascent structure	514:530	nascent structure of CETP (CETP-N) and CETP-G	514:558	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	1	72	theme	unglycosylated	184:197	arg1	structure					207:215	the unglycosylated crystal structure	180:215	the unglycosylated crystal structure	180:215	Current cholesteryl ester transfer protein (CETP) inhibitors are designed based on the unglycosylated crystal structure, and most of them have failed to cure cardiovascular disease (CVD).					
29726048	2	73	theme	glycosylation	340:352	arg1	structure					354:362	the glycosylation structure	336:362	the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP	336:439	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	8	74	dep	dynamics	1295:1302	arg1	the					1291:1293	the	1291:1293	the	1291:1293	Overall, these glycans affect the dynamics and structure of CETP through forming H-bonds with surrounding residues, and the sampled conformations of glycan is also affected by its surrounding residues.					
29726048	3	75	theme	new	612:614	arg1	light					616:620	new light	612:620	new light	612:620	Here, we used a total of 3.0-μs molecular dynamics (MD) trajectories of nascent structure of CETP (CETP-N) and CETP-G to study their structural differentiations, to shed new light on the CETP-mediated lipid exchange.					
29726048	2	76	from	effect	385:390	arg1	function					424:431	function	424:431	function	424:431	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
29726048	2	76	from	effect	385:390	arg1	structure					410:418	structure	410:418	structure	410:418	It is particularly important for us to investigate the glycosylation structure of CETP (CETP-G) and effect of glycans on the structure and function of CETP.					
30772453	0	0	theme	gene	106:109	arg1	clusters					111:118	native polysaccharide biosynthesis gene clusters	71:118	native polysaccharide biosynthesis gene clusters	71:118	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	4	1	from	machinery	954:962	arg1	plasmids					984:991	extrachromosomal plasmids	967:991	extrachromosomal plasmids	967:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	4	2	theme	glycosylation	940:952	arg1	machinery					954:962	the same glycosylation machinery	931:962	the same glycosylation machinery on extrachromosomal plasmids	931:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	5	3	theme	reliable	1222:1229	arg1	framework					1231:1239	a convenient and reliable framework	1205:1239	a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit	1205:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	2	4	theme	glyco-recoding	356:369	arg1	strategy					371:378	a glyco-recoding strategy	354:378	a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins	354:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	5	theme	glycoprotein	1156:1167	arg1	titers					1169:1174	higher glycoprotein titers	1149:1174	higher glycoprotein titers	1149:1174	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	5	6	theme	chemical	1309:1316	arg1	diversification					1318:1332	chemical diversification	1309:1332	chemical diversification of proteins	1309:1344	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	0	7	theme	biosynthesis	93:104	arg1	clusters					111:118	native polysaccharide biosynthesis gene clusters	71:118	native polysaccharide biosynthesis gene clusters	71:118	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	5	8	theme	bacterial	1245:1253	arg1	editing					1263:1269	bacterial glycome editing	1245:1269	bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit	1245:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	4	9	theme	same	935:938	arg1	machinery					954:962	the same glycosylation machinery	931:962	the same glycosylation machinery on extrachromosomal plasmids	931:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	5	10	theme	bacterial	1382:1390	arg1	toolkit					1409:1415	the bacterial glycoengineering toolkit	1378:1415	the bacterial glycoengineering toolkit	1378:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	2	11	theme	site-specific	576:588	arg1	conjugation					597:607	site-specific glycan conjugation	576:607	site-specific glycan conjugation to target proteins	576:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	5	12	theme	glycome	1255:1261	arg1	editing					1263:1269	bacterial glycome editing	1245:1269	bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit	1245:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	4	13	theme	E.	908:909	arg1	cells					916:920	E. coli cells	908:920	E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids	908:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	5	14	theme	glycoengineering	1392:1407	arg1	toolkit					1409:1415	the bacterial glycoengineering toolkit	1378:1415	the bacterial glycoengineering toolkit	1378:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	0	15	theme	clusters	111:118	arg1	editing					60:66	Recombineering-based genome editing	32:66	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.	0:119	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	3	16	theme	synthetic	779:787	arg1	DNA					789:791	synthetic DNA	779:791	synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation	779:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	5	17	theme	proteins	1337:1344	arg1	diversification					1318:1332	chemical diversification	1309:1332	chemical diversification of proteins	1309:1344	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	2	18	theme	structures	561:570	arg1	biosynthesis					525:536	biosynthesis	525:536	biosynthesis of heterologous glycan structures	525:570	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	2	18	theme	structures	561:570	arg1	conjugation					597:607	site-specific glycan conjugation	576:607	site-specific glycan conjugation to target proteins	576:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	3	19	theme	DNA	789:791	arg1	kbp					772:774	∼9-10 kbp	766:774	∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation	766:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	4	20	with	glycans	1025:1031	arg1	efficiency					1083:1092	equal or greater efficiency	1066:1092	equal or greater efficiency	1066:1092	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	2	21	theme	gene	423:426	arg1	clusters					428:435	major non-essential polysaccharide gene clusters	388:435	major non-essential polysaccharide gene clusters in K-12 Escherichia coli	388:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	2	22	theme	glycan	554:559	arg1	structures					561:570	heterologous glycan structures	541:570	heterologous glycan structures	541:570	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	23	theme	coli	911:914	arg1	cells					916:920	E. coli cells	908:920	E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids	908:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	3	24	link	N-linked	863:870	arg1	glycosylation					881:893	asparagine-linked (N-linked) protein glycosylation	844:893	asparagine-linked (N-linked) protein glycosylation	844:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	1	25	theme	large	203:207	arg1	segments					209:216	large segments	203:216	large segments of genomic DNA	203:231	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	0	26	theme	Escherichia	14:24	arg1	Glyco-recoded					0:12	Glyco-recoded	0:12	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.	0:119	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	4	27	theme	greater	1075:1081	arg1	efficiency					1083:1092	equal or greater efficiency	1066:1092	equal or greater efficiency	1066:1092	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	5	28	theme	stable	1292:1297	arg1	route					1299:1303	a more stable route	1285:1303	a more stable route for chemical diversification of proteins	1285:1344	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	2	29	theme	heterologous	541:552	arg1	structures					561:570	heterologous glycan structures	541:570	heterologous glycan structures	541:570	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	5	30	theme	convenient	1207:1216	arg1	framework					1231:1239	a convenient and reliable framework	1205:1239	a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit	1205:1415	Overall, our results define a convenient and reliable framework for bacterial glycome editing that provides a more stable route for chemical diversification of proteins in vivo and effectively expands the bacterial glycoengineering toolkit.					
30772453	2	31	theme	non-essential	394:406	arg1	clusters					428:435	major non-essential polysaccharide gene clusters	388:435	major non-essential polysaccharide gene clusters in K-12 Escherichia coli	388:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	32	theme	growth	1127:1132	arg1	phenotypes					1134:1143	markedly better growth phenotypes	1111:1143	markedly better growth phenotypes	1111:1143	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	3	33	theme	jejuni	816:821	arg1	enzymes					823:829	Campylobacter jejuni enzymes	802:829	Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation	802:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	4	34	theme	better	1120:1125	arg1	phenotypes					1134:1143	markedly better growth phenotypes	1111:1143	markedly better growth phenotypes	1111:1143	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	3	35	dep	antigen	677:683	arg1	loci					727:730	antigen loci	719:730	antigen loci	719:730	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	2	36	theme	major	388:392	arg1	clusters					428:435	major non-essential polysaccharide gene clusters	388:435	major non-essential polysaccharide gene clusters in K-12 Escherichia coli	388:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	37	theme	higher	1149:1154	arg1	titers					1169:1174	higher glycoprotein titers	1149:1174	higher glycoprotein titers	1149:1174	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	1	38	theme	functions	296:304	arg1	range					287:291	the range	283:291	the range of functions that an organism can perform	283:333	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	2	39	theme	target	612:617	arg1	proteins					619:626	target proteins	612:626	target proteins	612:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	1	40	theme	Recombineering-based	121:140	arg1	redesign					142:149	Recombineering-based redesign	121:149	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA	121:231	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	1	41	theme	genomic	221:227	arg1	DNA					229:231	genomic DNA	221:231	genomic DNA	221:231	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	2	42	from	clusters	428:435	arg1	K-12					440:443	K-12	440:443	K-12 Escherichia coli	440:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	1	43	theme	DNA	229:231	arg1	segments					209:216	large segments	203:216	large segments of genomic DNA	203:231	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	0	44	theme	genome	53:58	arg1	editing					60:66	Recombineering-based genome editing	32:66	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.	0:119	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	3	45	theme	Campylobacter	802:814	arg1	enzymes					823:829	Campylobacter jejuni enzymes	802:829	Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation	802:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	3	46	theme	enterobacterial	654:668	arg1	ECA					686:688	ECA	686:688	ECA	686:688	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	3	46	theme	enterobacterial	654:668	arg1	antigen					677:683	the native enterobacterial common antigen	643:683	the native enterobacterial common antigen (ECA)	643:689	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	0	47	theme	Recombineering-based	32:51	arg1	editing					60:66	Recombineering-based genome editing	32:66	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.	0:119	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	3	48	theme	asparagine-linked	844:860	arg1	glycosylation					881:893	asparagine-linked (N-linked) protein glycosylation	844:893	asparagine-linked (N-linked) protein glycosylation	844:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	2	49	theme	glycan	590:595	arg1	conjugation					597:607	site-specific glycan conjugation	576:607	site-specific glycan conjugation to target proteins	576:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	3	50	theme	N-linked	863:870	arg1	glycosylation					881:893	asparagine-linked (N-linked) protein glycosylation	844:893	asparagine-linked (N-linked) protein glycosylation	844:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	2	51	theme	glycosylation	491:503	arg1	components					505:514	orthogonal glycosylation components	480:514	orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins	480:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	52	theme	protein	1045:1051	arg1	targets					1053:1059	acceptor protein targets	1036:1059	acceptor protein targets	1036:1059	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	4	53	theme	equal	1066:1070	arg1	efficiency					1083:1092	equal or greater efficiency	1066:1092	equal or greater efficiency	1066:1092	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	2	54	dep	Escherichia	445:455	arg1	coli					457:460	coli	457:460	coli	457:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	2	55	theme	orthogonal	480:489	arg1	components					505:514	orthogonal glycosylation components	480:514	orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins	480:626	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	4	56	theme	extrachromosomal	967:982	arg1	plasmids					984:991	extrachromosomal plasmids	967:991	extrachromosomal plasmids	967:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	4	57	gly	glycoprotein	1156:1167	arg1	glycoprotein					1156:1167	higher glycoprotein titers	1149:1174	higher glycoprotein titers	1149:1174	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	3	58	theme	native	647:652	arg1	ECA					686:688	ECA	686:688	ECA	686:688	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	3	58	theme	native	647:652	arg1	antigen					677:683	the native enterobacterial common antigen	643:683	the native enterobacterial common antigen (ECA)	643:689	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	3	59	link	asparagine-linked	844:860	arg1	glycosylation					881:893	asparagine-linked (N-linked) protein glycosylation	844:893	asparagine-linked (N-linked) protein glycosylation	844:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	1	60	theme	bacterial	154:162	arg1	genomes					164:170	bacterial genomes	154:170	bacterial genomes	154:170	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	4	61	contain	carrying	922:929	arg2	machinery					954:962	the same glycosylation machinery	931:962	the same glycosylation machinery on extrachromosomal plasmids	931:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	4	61	contain	carrying	922:929	arg1	cells					916:920	E. coli cells	908:920	E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids	908:991	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	4	62	theme	glyco-recoded	994:1006	arg1	strains					1008:1014	glyco-recoded strains	994:1014	glyco-recoded strains	994:1014	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
30772453	3	63	theme	common	670:675	arg1	ECA					686:688	ECA	686:688	ECA	686:688	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	3	63	theme	common	670:675	arg1	antigen					677:683	the native enterobacterial common antigen	643:683	the native enterobacterial common antigen (ECA)	643:689	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	1	64	theme	genomes	164:170	arg1	redesign					142:149	Recombineering-based redesign	121:149	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA	121:231	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	3	65	theme	protein	873:879	arg1	glycosylation					881:893	asparagine-linked (N-linked) protein glycosylation	844:893	asparagine-linked (N-linked) protein glycosylation	844:893	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	0	66	theme	polysaccharide	78:91	arg1	clusters					111:118	native polysaccharide biosynthesis gene clusters	71:118	native polysaccharide biosynthesis gene clusters	71:118	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	3	67	theme	antigen	719:725	arg1	loci					727:730	antigen loci	719:730	antigen loci	719:730	Specifically, the native enterobacterial common antigen (ECA) and O-polysaccharide (O-PS) antigen loci were systematically replaced with ∼9-10 kbp of synthetic DNA encoding Campylobacter jejuni enzymes required for asparagine-linked (N-linked) protein glycosylation.					
30772453	0	68	dep	Escherichia	14:24	arg1	coli					26:29	coli	26:29	coli	26:29	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	2	69	theme	Escherichia	445:455	arg1	K-12					440:443	K-12	440:443	K-12 Escherichia coli	440:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	2	70	theme	polysaccharide	408:421	arg1	clusters					428:435	major non-essential polysaccharide gene clusters	388:435	major non-essential polysaccharide gene clusters in K-12 Escherichia coli	388:460	Here, we describe a glyco-recoding strategy whereby major non-essential polysaccharide gene clusters in K-12 Escherichia coli are replaced with orthogonal glycosylation components for both biosynthesis of heterologous glycan structures and site-specific glycan conjugation to target proteins.					
30772453	1	71	theme	powerful	250:257	arg1	technique					259:267	a powerful technique	248:267	a powerful technique for expanding the range of functions that an organism can perform	248:333	Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform.					
30772453	0	72	theme	native	71:76	arg1	clusters					111:118	native polysaccharide biosynthesis gene clusters	71:118	native polysaccharide biosynthesis gene clusters	71:118	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	0	73	dep	Glyco-recoded	0:12	arg1	editing					60:66	Recombineering-based genome editing	32:66	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.	0:119	Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.					
30772453	4	74	theme	acceptor	1036:1043	arg1	targets					1053:1059	acceptor protein targets	1036:1059	acceptor protein targets	1036:1059	Compared to E. coli cells carrying the same glycosylation machinery on extrachromosomal plasmids, glyco-recoded strains attached glycans to acceptor protein targets with equal or greater efficiency while exhibiting markedly better growth phenotypes and higher glycoprotein titers.					
29512066	1	0	attach	present	86:92	arg2	we					83:84	we	83:84	we	83:84	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	1	0	attach	present	86:92	arg1	chapter					74:80	this chapter	69:80	this chapter	69:80	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	4	1	theme	protein	736:742	arg1	pathway					767:773	either the protein N-linked glycosylation pathway	725:773	pathway	767:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	3	2	theme	glycan	483:488	arg1	pool					490:493	The glycan pool	479:493	The glycan pool	479:493	The glycan pool is subsequently permethylated to increase mass spectrometry sensitivity.					
29512066	3	2	theme	glycan	483:488	arg1	permethylated					511:523	permethylated	511:523	permethylated	511:523	The glycan pool is subsequently permethylated to increase mass spectrometry sensitivity.					
29512066	5	3	from	spectra	858:864	arg1	mode					879:882	reflector mode	869:882	reflector mode	869:882	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	2	4	with	digestion	294:302	arg1	F					331:331	peptide-N-glycosidase F	309:331	peptide-N-glycosidase F (PNGase F)	309:342	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	4	with	digestion	294:302	arg1	F					341:341	PNGase F	334:341	PNGase F	334:341	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	5	gly	glycoproteins	277:289	arg1	glycoproteins					277:289	denatured carboxymethylated glycoproteins	249:289	denatured carboxymethylated glycoproteins	249:289	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	6	theme	mass	671:674	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	6	theme	mass	671:674	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	3	7	theme	mass	537:540	arg1	sensitivity					555:565	mass spectrometry sensitivity	537:565	mass spectrometry sensitivity	537:565	The glycan pool is subsequently permethylated to increase mass spectrometry sensitivity.					
29512066	2	8	theme	carboxymethylated	259:275	arg1	glycoproteins					277:289	denatured carboxymethylated glycoproteins	249:289	denatured carboxymethylated glycoproteins	249:289	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	9	theme	MALDI	690:694	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	9	theme	MALDI	690:694	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	10	theme	TOF	784:786	arg1	MS/MS					788:792	MALDI TOF MS/MS	778:792	MALDI TOF MS/MS	778:792	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	11	theme	denatured	249:257	arg1	glycoproteins					277:289	denatured carboxymethylated glycoproteins	249:289	denatured carboxymethylated glycoproteins	249:289	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	12	theme	C18	368:370	arg1	Sep-Pak®					372:379	C18 Sep-Pak®	368:379	C18 Sep-Pak®	368:379	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	5	13	theme	optimal	892:898	arg1	ratio					916:920	an optimal signal-to-noise ratio	889:920	an optimal signal-to-noise ratio up to m/z 4800	889:935	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	0	14	theme	CSF	0:2	arg1	N-Glycomics					4:14	CSF N-Glycomics	0:14	CSF N-Glycomics	0:14	CSF N-Glycomics Using MALDI MS Techniques in Alzheimer's Disease.					
29512066	4	15	theme	TOF	696:698	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	15	theme	TOF	696:698	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	16	theme	Hypercarb™	427:436	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	6	17	theme	specific	993:1000	arg1	profiles					1012:1019	specific N-glycome profiles	993:1019	specific N-glycome profiles	993:1019	This method has been successfully adopted to associate specific N-glycome profiles to the early and the advanced phases of Alzheimer's disease.					
29512066	2	18	theme	peptide-N-glycosidase	309:329	arg1	F					331:331	peptide-N-glycosidase F	309:331	peptide-N-glycosidase F (PNGase F)	309:342	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	18	theme	peptide-N-glycosidase	309:329	arg1	F					341:341	PNGase F	334:341	PNGase F	334:341	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	1	19	theme	structural	154:163	arg1	elucidation					165:175	the structural elucidation	150:175	the structural elucidation of the cerebrospinal fluid (CSF) N-glycome	150:218	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	2	20	theme	HyperSep™	417:425	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	21	theme	MALDI	778:782	arg1	MS/MS					788:792	MALDI TOF MS/MS	778:792	MALDI TOF MS/MS	778:792	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	0	22	theme	MALDI	22:26	arg1	Techniques					31:40	MALDI MS Techniques	22:40	MALDI MS Techniques in Alzheimer's Disease	22:63	CSF N-Glycomics Using MALDI MS Techniques in Alzheimer's Disease.					
29512066	5	23	theme	mass	853:856	arg1	spectra					858:864	high-quality mass spectra	840:864	high-quality mass spectra in reflector mode	840:882	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	6	24	theme	N-glycome	1002:1010	arg1	profiles					1012:1019	specific N-glycome profiles	993:1019	specific N-glycome profiles	993:1019	This method has been successfully adopted to associate specific N-glycome profiles to the early and the advanced phases of Alzheimer's disease.					
29512066	3	25	theme	spectrometry	542:553	arg1	sensitivity					555:565	mass spectrometry sensitivity	537:565	mass spectrometry sensitivity	537:565	The glycan pool is subsequently permethylated to increase mass spectrometry sensitivity.					
29512066	4	26	theme	spectrometry	676:687	arg1	analysis					704:711	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis	612:711	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	27	theme	PGC	412:414	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	28	theme	Molecular	568:576	arg1	assignments					578:588	Molecular assignments	568:588	Molecular assignments	568:588	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	29	theme	carbon	404:409	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	30	theme	SPE	462:464	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	31	theme	N-linked	744:751	arg1	pathway					767:773	either the protein N-linked glycosylation pathway	725:773	pathway	767:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	32	theme	graphitized	392:402	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	1	33	theme	cerebrospinal	184:196	arg1	CSF					205:207	CSF	205:207	CSF	205:207	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	1	33	theme	cerebrospinal	184:196	arg1	fluid					198:202	cerebrospinal fluid	184:202	the cerebrospinal fluid (CSF) N-glycome	180:218	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	0	34	theme	MS	28:29	arg1	Techniques					31:40	MALDI MS Techniques	22:40	MALDI MS Techniques in Alzheimer's Disease	22:63	CSF N-Glycomics Using MALDI MS Techniques in Alzheimer's Disease.					
29512066	6	35	theme	advanced	1042:1049	arg1	phases					1051:1056	the advanced phases	1038:1056	the advanced phases of Alzheimer's disease	1038:1079	This method has been successfully adopted to associate specific N-glycome profiles to the early and the advanced phases of Alzheimer's disease.					
29512066	1	36	theme	fluid	198:202	arg1	N-glycome					210:218	the cerebrospinal fluid (CSF) N-glycome	180:218	the cerebrospinal fluid (CSF) N-glycome	180:218	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
29512066	2	37	theme	extraction	450:459	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	38	theme	glycosylation	753:765	arg1	pathway					767:773	either the protein N-linked glycosylation pathway	725:773	pathway	767:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	39	theme	solid-phase	438:448	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	40	theme	PNGase	334:339	arg1	F					331:331	peptide-N-glycosidase F	309:331	peptide-N-glycosidase F (PNGase F)	309:342	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	40	theme	PNGase	334:339	arg1	F					341:341	PNGase F	334:341	PNGase F	334:341	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	4	41	dep	analysis	704:711	arg1	data					794:797	data	794:797	data	794:797	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	42	theme	laser	628:632	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	42	theme	laser	628:632	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	43	link	N-linked	744:751	arg1	pathway					767:773	either the protein N-linked glycosylation pathway	725:773	pathway	767:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	44	dep	pathway	767:773	arg1	either					725:730	either	725:730	either	725:730	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	2	45	attach	released	235:242	arg2	N-glycans					221:229	N-glycans	221:229	N-glycans	221:229	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	45	attach	released	235:242	arg1	glycoproteins					277:289	denatured carboxymethylated glycoproteins	249:289	denatured carboxymethylated glycoproteins	249:289	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	2	46	theme	porous	385:390	arg1	cartridges					467:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges	385:476	N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges.					
29512066	5	47	theme	reflector	869:877	arg1	mode					879:882	reflector mode	869:882	reflector mode	869:882	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	5	48	theme	signal-to-noise	900:914	arg1	ratio					916:920	an optimal signal-to-noise ratio	889:920	an optimal signal-to-noise ratio up to m/z 4800	889:935	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	4	49	theme	time-of-flight	656:669	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	49	theme	time-of-flight	656:669	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	50	theme	desorption/ionization	634:654	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	50	theme	desorption/ionization	634:654	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	51	theme	matrix-assisted	612:626	arg1	MS					700:701	MALDI TOF MS	690:701	MALDI TOF MS	690:701	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	4	51	theme	matrix-assisted	612:626	arg1	spectrometry					676:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	612:687	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway	612:773	Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data.					
29512066	5	52	theme	high-quality	840:851	arg1	spectra					858:864	high-quality mass spectra	840:864	high-quality mass spectra in reflector mode	840:882	Each stage has been optimized to obtain high-quality mass spectra in reflector mode with an optimal signal-to-noise ratio up to m/z 4800.					
29512066	1	53	theme	N-glycome	210:218	arg1	elucidation					165:175	the structural elucidation	150:175	the structural elucidation of the cerebrospinal fluid (CSF) N-glycome	150:218	In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome.					
31838191	4	0	contain	contain	768:774	arg2	group					786:790	a hydroxy group	776:790	a hydroxy group at the 6-position	776:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	0	contain	contain	768:774	arg1	thiogalactosides					746:761	thiogalactosides	746:761	thiogalactosides that contain a hydroxy group at the 6-position	746:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	1	theme	cyclic	938:943	arg1	oligosaccharides					945:960	cyclic oligosaccharides	938:960	cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products	938:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	2	theme	tri~penta	979:987	arg1	galactosides					989:1000	tri~penta galactosides	979:1000	tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products	979:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	3	theme	current	410:416	arg1	routes					440:445	the current traditional synthetic routes	406:445	the current traditional synthetic routes	406:445	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	1	4	theme	excellent	165:173	arg1	molecules					180:188	excellent host molecules	165:188	excellent host molecules for the inclusion of various organic guest molecules	165:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	4	theme	excellent	165:173	arg1	oligosaccharides					100:115	Cyclic oligosaccharides	93:115	Cyclic oligosaccharides such as cyclodextrins (CyDs)	93:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	4	theme	excellent	165:173	arg1	cyclodextrins					125:137	cyclodextrins	125:137	cyclodextrins (CyDs)	125:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	4	5	theme	cyclic	1134:1139	arg1	tetragalactoside					1141:1156	the cyclic tetragalactoside	1130:1156	the cyclic tetragalactoside	1130:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	6	theme	cyclic	299:304	arg1	oligosaccharides					306:321	cyclic oligosaccharides	299:321	cyclic oligosaccharides from simple and readily available glycosyl donors	299:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	7	theme	solvents	700:707	arg1	series					676:681	A series	674:681	A series of promoters and solvents	674:707	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	5	8	from	effect	1163:1168	arg1	products					1243:1250	the glycosylation products	1225:1250	the glycosylation products	1225:1250	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	4	9	gly	glycosylation	729:741	arg1	thiogalactosides					746:761	thiogalactosides	746:761	thiogalactosides that contain a hydroxy group at the 6-position	746:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	10	from	donors	366:371	arg1	oligosaccharides					306:321	cyclic oligosaccharides	299:321	cyclic oligosaccharides from simple and readily available glycosyl donors	299:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	11	theme	single	1087:1092	arg1	analysis					1118:1125	a single crystal X-ray structure analysis	1085:1125	a single crystal X-ray structure analysis of the cyclic tetragalactoside	1085:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	5	12	theme	reaction	1173:1180	arg1	temperature					1182:1192	reaction temperature	1173:1192	reaction temperature	1173:1192	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	4	13	theme	N-iodosuccinimide	837:853	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	6	14	theme	thioglucosides	1339:1352	arg1	glycosylation					1318:1330	the glycosylation	1314:1330	the glycosylation of the thioglucosides	1314:1352	The cyclic glucosides were obtained by the glycosylation of the thioglucosides.					
31838191	1	15	theme	host	175:178	arg1	molecules					180:188	excellent host molecules	165:188	excellent host molecules for the inclusion of various organic guest molecules	165:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	15	theme	host	175:178	arg1	oligosaccharides					100:115	Cyclic oligosaccharides	93:115	Cyclic oligosaccharides such as cyclodextrins (CyDs)	93:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	15	theme	host	175:178	arg1	cyclodextrins					125:137	cyclodextrins	125:137	cyclodextrins (CyDs)	125:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	4	16	theme	glycosidic	1031:1040	arg1	linkage					1042:1048	repeating β-(1→6) glycosidic linkage	1013:1048	repeating β-(1→6) glycosidic linkage	1013:1048	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	16	theme	glycosidic	1031:1040	arg1	products					1059:1066	major products	1053:1066	major products	1053:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	17	from	system	903:908	arg1	dichloromethane					913:927	dichloromethane	913:927	dichloromethane	913:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	18	theme	promoter	894:901	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	19	theme	methods	277:283	arg1	development					248:258	The development	244:258	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors	244:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	2	19	theme	methods	277:283	arg1	desirable					389:397	desirable	389:397	desirable	389:397	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	7	20	theme	deprotected	1449:1459	arg1	tetragalactoside					1468:1483	deprotected cyclic tetragalactoside	1449:1483	deprotected cyclic tetragalactoside	1449:1483	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	4	21	theme	crystal	1094:1100	arg1	analysis					1118:1125	a single crystal X-ray structure analysis	1085:1125	a single crystal X-ray structure analysis of the cyclic tetragalactoside	1085:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	3	22	theme	cyclic	561:566	arg1	oligosaccharides					568:583	cyclic oligosaccharides	561:583	cyclic oligosaccharides	561:583	We herein report on the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides, typically, 2,3,4-protected 1-thioglycosides.					
31838191	7	23	theme	synthesized	1390:1400	arg1	tetragalactoside					1409:1424	the synthesized cyclic tetragalactoside	1386:1424	the synthesized cyclic tetragalactoside	1386:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	4	24	theme	major	1053:1057	arg1	linkage					1042:1048	repeating β-(1→6) glycosidic linkage	1013:1048	repeating β-(1→6) glycosidic linkage	1013:1048	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	24	theme	major	1053:1057	arg1	products					1059:1066	major products	1053:1066	major products	1053:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	3	25	theme	monothioglycosides	609:626	arg1	polyglycosylation					588:604	polyglycosylation	588:604	polyglycosylation of monothioglycosides	588:626	We herein report on the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides, typically, 2,3,4-protected 1-thioglycosides.					
31838191	6	26	gly	glycosylation	1318:1330	arg1	thioglucosides					1339:1352	the thioglucosides	1335:1352	the thioglucosides	1335:1352	The cyclic glucosides were obtained by the glycosylation of the thioglucosides.					
31838191	0	27	theme	One-pot	0:6	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides	0:90	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides.					
31838191	2	28	dep	steps	473:477	arg1	reactions					494:502	glycosylation reactions	480:502	glycosylation reactions	480:502	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	2	28	dep	steps	473:477	arg1	deprotections					508:520	deprotections	508:520	deprotections	508:520	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	29	from	6-position	799:808	arg1	group					786:790	a hydroxy group	776:790	a hydroxy group at the 6-position	776:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	30	theme	simple	328:333	arg1	donors					366:371	simple and readily available glycosyl donors	328:371	simple and readily available glycosyl donors	328:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	0	31	theme	cyclic	21:26	arg1	oligosaccharides					28:43	cyclic oligosaccharides	21:43	cyclic oligosaccharides	21:43	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides.					
31838191	7	32	theme	cyclic	1461:1466	arg1	tetragalactoside					1468:1483	deprotected cyclic tetragalactoside	1449:1483	deprotected cyclic tetragalactoside	1449:1483	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	7	33	theme	tetragalactoside	1409:1424	arg1	tetragalactoside					1409:1424	the synthesized cyclic tetragalactoside	1386:1424	the synthesized cyclic tetragalactoside	1386:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	7	33	theme	tetragalactoside	1409:1424	arg1	groups					1376:1381	protecting groups	1365:1381	protecting groups of the synthesized cyclic tetragalactoside	1365:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	2	34	theme	multiple	455:462	arg1	steps					473:477	multiple reaction steps	455:477	multiple reaction steps (glycosylation reactions and deprotections)	455:521	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	5	35	theme	concentrations	1207:1220	arg1	effect					1163:1168	The effect	1159:1168	The effect of reaction temperature and reactant concentrations on the glycosylation products	1159:1250	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	6	36	theme	cyclic	1279:1284	arg1	glucosides					1286:1295	The cyclic glucosides	1275:1295	The cyclic glucosides	1275:1295	The cyclic glucosides were obtained by the glycosylation of the thioglucosides.					
31838191	4	37	contain	containing	1002:1011	arg1	galactosides					989:1000	tri~penta galactosides	979:1000	tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products	979:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	37	contain	containing	1002:1011	arg2	linkage					1042:1048	repeating β-(1→6) glycosidic linkage	1013:1048	repeating β-(1→6) glycosidic linkage	1013:1048	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	37	contain	containing	1002:1011	arg2	products					1059:1066	major products	1053:1066	major products	1053:1066	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	38	theme	promoters	686:694	arg1	series					676:681	A series	674:681	A series of promoters and solvents	674:707	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	7	39	theme	cyclic	1402:1407	arg1	tetragalactoside					1409:1424	the synthesized cyclic tetragalactoside	1386:1424	the synthesized cyclic tetragalactoside	1386:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	1	40	theme	various	211:217	arg1	molecules					233:241	various organic guest molecules	211:241	various organic guest molecules	211:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	3	41	theme	2,3,4-protected	640:654	arg1	1-thioglycosides					656:671	2,3,4-protected 1-thioglycosides	640:671	2,3,4-protected 1-thioglycosides	640:671	We herein report on the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides, typically, 2,3,4-protected 1-thioglycosides.					
31838191	4	42	theme	structure	1108:1116	arg1	analysis					1118:1125	a single crystal X-ray structure analysis	1085:1125	a single crystal X-ray structure analysis of the cyclic tetragalactoside	1085:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	43	theme	synthetic	430:438	arg1	routes					440:445	the current traditional synthetic routes	406:445	the current traditional synthetic routes	406:445	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	0	44	theme	oligosaccharides	28:43	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides	0:90	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides.					
31838191	5	45	theme	glycosylation	1229:1241	arg1	products					1243:1250	the glycosylation products	1225:1250	the glycosylation products	1225:1250	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	2	46	theme	reaction	464:471	arg1	steps					473:477	multiple reaction steps	455:477	multiple reaction steps (glycosylation reactions and deprotections)	455:521	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	1	47	theme	organic	219:225	arg1	molecules					233:241	various organic guest molecules	211:241	various organic guest molecules	211:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	4	48	theme	X-ray	1102:1106	arg1	analysis					1118:1125	a single crystal X-ray structure analysis	1085:1125	a single crystal X-ray structure analysis of the cyclic tetragalactoside	1085:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	5	49	theme	temperature	1182:1192	arg1	effect					1163:1168	The effect	1159:1168	The effect of reaction temperature and reactant concentrations on the glycosylation products	1159:1250	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	4	50	theme	hydroxy	778:784	arg1	group					786:790	a hydroxy group	776:790	a hydroxy group at the 6-position	776:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	51	theme	glycosylation	480:492	arg1	reactions					494:502	glycosylation reactions	480:502	glycosylation reactions	480:502	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	5	52	theme	reactant	1198:1205	arg1	concentrations					1207:1220	reactant concentrations	1198:1220	reactant concentrations	1198:1220	The effect of reaction temperature and reactant concentrations on the glycosylation products was also investigated.					
31838191	4	53	theme	TMSOTf	886:891	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	54	theme	glycosyl	357:364	arg1	donors					366:371	simple and readily available glycosyl donors	328:371	simple and readily available glycosyl donors	328:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	55	theme	NIS	856:858	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	56	theme	synthetic	267:275	arg1	methods					277:283	new synthetic methods	263:283	new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors	263:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	57	theme	triflate	876:883	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	2	58	theme	available	347:355	arg1	donors					366:371	simple and readily available glycosyl donors	328:371	simple and readily available glycosyl donors	328:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	59	theme	tetragalactoside	1141:1156	arg1	analysis					1118:1125	a single crystal X-ray structure analysis	1085:1125	a single crystal X-ray structure analysis of the cyclic tetragalactoside	1085:1156	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	1	60	theme	guest	227:231	arg1	molecules					233:241	various organic guest molecules	211:241	various organic guest molecules	211:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	61	theme	Cyclic	93:98	arg1	molecules					180:188	excellent host molecules	165:188	excellent host molecules for the inclusion of various organic guest molecules	165:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	61	theme	Cyclic	93:98	arg1	oligosaccharides					100:115	Cyclic oligosaccharides	93:115	Cyclic oligosaccharides such as cyclodextrins (CyDs)	93:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	1	61	theme	Cyclic	93:98	arg1	cyclodextrins					125:137	cyclodextrins	125:137	cyclodextrins (CyDs)	125:144	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	2	62	theme	new	263:265	arg1	methods					277:283	new synthetic methods	263:283	new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors	263:371	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	3	63	gly	polyglycosylation	588:604	arg1	monothioglycosides					609:626	monothioglycosides	609:626	monothioglycosides	609:626	We herein report on the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides, typically, 2,3,4-protected 1-thioglycosides.					
31838191	1	64	theme	molecules	233:241	arg1	inclusion					198:206	the inclusion	194:206	the inclusion of various organic guest molecules	194:241	Cyclic oligosaccharides such as cyclodextrins (CyDs) have been known as excellent host molecules for the inclusion of various organic guest molecules.					
31838191	0	65	theme	monothioglycosides	73:90	arg1	polyglycosylation					52:68	the polyglycosylation	48:68	the polyglycosylation of monothioglycosides	48:90	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides.					
31838191	2	66	theme	traditional	418:428	arg1	routes					440:445	the current traditional synthetic routes	406:445	the current traditional synthetic routes	406:445	The development of new synthetic methods for preparing cyclic oligosaccharides from simple and readily available glycosyl donors would be highly desirable, since the current traditional synthetic routes include multiple reaction steps (glycosylation reactions and deprotections).					
31838191	4	67	theme	/trimethylsilyl	860:874	arg1	system					903:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system	835:908	a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane	835:927	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	4	68	theme	thiogalactosides	746:761	arg1	glycosylation					729:741	the glycosylation	725:741	the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position	725:808	A series of promoters and solvents were tested for the glycosylation of thiogalactosides that contain a hydroxy group at the 6-position, and glycosylation using a N-iodosuccinimide (NIS)/trimethylsilyl triflate (TMSOTf) promoter system in dichloromethane afforded cyclic oligosaccharides which consist of tri~penta galactosides containing repeating β-(1→6) glycosidic linkage as major products, as evidenced by a single crystal X-ray structure analysis of the cyclic tetragalactoside.					
31838191	3	69	theme	oligosaccharides	568:583	arg1	synthesis					548:556	the synthesis	544:556	the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides	544:626	We herein report on the synthesis of cyclic oligosaccharides by polyglycosylation of monothioglycosides, typically, 2,3,4-protected 1-thioglycosides.					
31838191	0	70	gly	polyglycosylation	52:68	arg1	monothioglycosides					73:90	monothioglycosides	73:90	monothioglycosides	73:90	One-pot synthesis of cyclic oligosaccharides by the polyglycosylation of monothioglycosides.					
31838191	7	71	theme	protecting	1365:1374	arg1	tetragalactoside					1409:1424	the synthesized cyclic tetragalactoside	1386:1424	the synthesized cyclic tetragalactoside	1386:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31838191	7	71	theme	protecting	1365:1374	arg1	groups					1376:1381	protecting groups	1365:1381	protecting groups of the synthesized cyclic tetragalactoside	1365:1424	Moreover, protecting groups of the synthesized cyclic tetragalactoside were removed to obtain deprotected cyclic tetragalactoside.					
31453603	10	0	theme	mass	2121:2124	arg1	space					2139:2143	mass vs. mobility space	2121:2143	space	2139:2143	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	3	1	theme	gas	710:712	arg1	phase					714:718	the gas phase	706:718	the gas phase	706:718	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	1	2	theme	significant	259:269	arg1	interest					271:278	significant interest	259:278	significant interest	259:278	Ion mobility spectrometry (IMS) is of significant interest as a platform for glycoanalysis.					
31453603	9	3	theme	experimental	1668:1679	arg1	consistent					1699:1708	consistent	1699:1708	consistent	1699:1708	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	3	theme	experimental	1668:1679	arg1	observations					1681:1692	the experimental observations	1664:1692	the experimental observations	1664:1692	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	4	theme	solvation	202:210	arg1	effects					212:218	charge solvation effects	195:218	charge solvation effects	195:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	6	5	theme	analytes	1198:1205	arg1	characteristics					1175:1189	the physicochemical characteristics	1155:1189	the physicochemical characteristics of the analytes	1155:1205	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	10	6	theme	mobility	2130:2137	arg1	space					2139:2143	mass vs. mobility space	2121:2143	space	2139:2143	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	2	7	theme	much	319:322	arg1	attention					324:332	much attention	319:332	much attention	319:332	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	9	8	theme	glycan	1717:1722	arg1	size					1724:1727	a glycan size	1715:1727	a glycan size	1715:1727	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	1	9	theme	Ion	221:223	arg1	IMS					248:250	IMS	248:250	IMS	248:250	Ion mobility spectrometry (IMS) is of significant interest as a platform for glycoanalysis.					
31453603	1	9	theme	Ion	221:223	arg1	spectrometry					234:245	Ion mobility spectrometry	221:245	Ion mobility spectrometry (IMS)	221:251	Ion mobility spectrometry (IMS) is of significant interest as a platform for glycoanalysis.					
31453603	2	10	theme	space	548:552	arg1	regions					519:525	distinct regions	510:525	distinct regions of mass vs. mobility space	510:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	8	11	theme	glycopeptide	1471:1482	arg1	CCSs					1484:1487	glycopeptide CCSs	1471:1487	glycopeptide CCSs	1471:1487	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	5	12	gly	glycopeptide	934:945	arg2	glycopeptide					934:945	model protonated glycopeptide ions	917:950	model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	917:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	8	13	theme	glycan	1417:1422	arg1	group					1424:1428	the glycan group	1413:1428	the glycan group	1413:1428	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	0	14	theme	coulombic	171:179	arg1	repulsion					181:189	coulombic repulsion	171:189	coulombic repulsion	171:189	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	5	15	theme	varied	972:977	arg1	topologies					995:1004	systematically varied oligosaccharide topologies	957:1004	systematically varied oligosaccharide topologies	957:1004	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	8	16	dep	increase	1450:1457	arg1	CCSs					1484:1487	glycopeptide CCSs	1471:1487	glycopeptide CCSs	1471:1487	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	2	17	theme	distinct	510:517	arg1	regions					519:525	distinct regions	510:525	distinct regions of mass vs. mobility space	510:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	8	18	theme	charge	1510:1515	arg1	state					1517:1521	the ion charge state	1502:1521	the ion charge state	1502:1521	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	0	19	theme	collision	58:66	arg1	sections					74:81	the ion-neutral collision cross sections	42:81	the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects	42:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	0	20	theme	charge	16:21	arg1	state					23:27	charge state	16:27	charge state	16:27	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	5	21	theme	model	917:921	arg1	ions					947:950	model protonated glycopeptide ions	917:950	model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	917:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	2	22	theme	biomolecules	492:503	arg1	classes					481:487	different classes	471:487	different classes of biomolecules	471:503	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	5	23	theme	glycopeptide	934:945	arg1	ions					947:950	model protonated glycopeptide ions	917:950	model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	917:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	9	24	theme	gas	1594:1596	arg1	structures					1604:1613	gas phase structures	1594:1613	gas phase structures	1594:1613	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	25	theme	glycopeptides	106:118	arg1	sections					74:81	the ion-neutral collision cross sections	42:81	the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects	42:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	5	26	theme	charge	1034:1039	arg1	states					1041:1046	charge states	1034:1046	charge states	1034:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	7	27	theme	dependent	1367:1375	arg1	fashion					1377:1383	a charge state dependent fashion	1352:1383	a charge state dependent fashion	1352:1383	Glycan size and composition emerged as a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion.					
31453603	5	28	with	ions	947:950	arg1	states					1041:1046	charge states	1034:1046	charge states	1034:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	5	28	with	ions	947:950	arg1	topologies					995:1004	systematically varied oligosaccharide topologies	957:1004	systematically varied oligosaccharide topologies	957:1004	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	5	28	with	ions	947:950	arg1	sequences					1019:1027	polypeptide sequences	1007:1027	polypeptide sequences	1007:1027	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	0	29	dep	sections	74:81	arg1	effects					212:218	charge solvation effects	195:218	charge solvation effects	195:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	0	29	dep	sections	74:81	arg1	deconstruction					153:166	an experimental and theoretical deconstruction	121:166	an experimental and theoretical deconstruction of coulombic repulsion	121:189	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	9	30	theme	extended	1843:1850	arg1	structures					1852:1861	more extended structures	1838:1861	more extended structures	1838:1861	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	4	31	theme	glycopeptides	780:792	arg1	characteristics					761:775	the physical and chemical characteristics	735:775	the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci	735:834	Nevertheless, the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci have yet to be systematically investigated.					
31453603	7	32	theme	Glycan	1208:1213	arg1	size					1215:1218	Glycan size	1208:1218	Glycan size	1208:1218	Glycan size and composition emerged as a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion.					
31453603	5	33	theme	polypeptide	1007:1017	arg1	sequences					1019:1027	polypeptide sequences	1007:1027	polypeptide sequences	1007:1027	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	2	34	theme	appealing	424:432	arg1	ability					455:461	the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space	451:552	the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space	451:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	2	34	theme	appealing	424:432	arg1	aspect					434:439	another appealing aspect	416:439	another appealing aspect of IMS	416:446	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	9	35	theme	structures	1604:1613	arg1	simulations					1575:1585	Molecular dynamics (MD) simulations	1551:1585	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides	1551:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	6	36	theme	collision	1078:1086	arg1	sections					1094:1101	over 110 ion-neutral collision cross sections	1057:1101	over 110 ion-neutral collision cross sections (CCSs)	1057:1108	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	6	36	theme	collision	1078:1086	arg1	CCSs					1104:1107	CCSs	1104:1107	CCSs	1104:1107	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	10	37	from	applications	2149:2160	arg1	glycoproteomics					2165:2179	glycoproteomics	2165:2179	glycoproteomics	2165:2179	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	7	38	gly	glycopeptides	1308:1320	arg2	glycopeptides					1308:1320	the glycopeptides	1304:1320	the glycopeptides	1304:1320	Glycan size and composition emerged as a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion.					
31453603	9	39	theme	CCSs	1619:1622	arg1	simulations					1575:1585	Molecular dynamics (MD) simulations	1551:1585	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides	1551:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	40	theme	solvation	1887:1895	arg1	effects					1897:1903	charge solvation effects	1880:1903	charge solvation effects in which the glycan plays a major role (tending to result in more compact structures)	1880:1989	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	4	41	theme	mobility	822:829	arg1	loci					831:834	mobility loci	822:834	mobility loci	822:834	Nevertheless, the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci have yet to be systematically investigated.					
31453603	9	42	theme	selected	1627:1634	arg1	glycopeptides					1636:1648	selected glycopeptides	1627:1648	selected glycopeptides	1627:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	2	43	theme	isomeric	372:379	arg1	carbohydrates					381:393	isomeric carbohydrates	372:393	isomeric carbohydrates	372:393	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	0	44	link	N-linked	97:104	arg1	glycopeptides					106:118	protonated N-linked glycopeptides	86:118	protonated N-linked glycopeptides	86:118	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	9	45	theme	repulsion	1798:1806	arg1	effects					1808:1814	coulombic repulsion effects	1788:1814	coulombic repulsion effects (tending to result in more extended structures)	1788:1862	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	46	theme	charge	195:200	arg1	effects					212:218	charge solvation effects	195:218	charge solvation effects	195:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	7	47	theme	CCS	1282:1284	arg1	space					1286:1290	the CCS space	1278:1290	the CCS space occupied by the glycopeptides	1278:1320	Glycan size and composition emerged as a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion.					
31453603	9	48	theme	major	1933:1937	arg1	role					1939:1942	a major role	1931:1942	a major role (tending to result in more compact structures)	1931:1989	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	3	49	gly	non-glycosylated	650:665	arg1	peptides					667:674	glycosylated and non-glycosylated peptides	633:674	glycosylated and non-glycosylated peptides	633:674	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	8	50	gly	glycopeptide	1471:1482	arg2	glycopeptide					1471:1482	glycopeptide CCSs	1471:1487	glycopeptide CCSs	1471:1487	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	10	51	theme	space	2139:2143	arg1	regions					2110:2116	glycopeptide-enriched regions	2088:2116	glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics	2088:2179	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	3	52	gly	glycosylated	633:644	arg1	peptides					667:674	glycosylated and non-glycosylated peptides	633:674	glycosylated and non-glycosylated peptides	633:674	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	5	53	theme	IMS	904:906	arg1	study					908:912	an IMS study	901:912	an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	901:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	9	54	dep	structures	1604:1613	arg1	the					1590:1592	the	1590:1592	the	1590:1592	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	55	theme	experimental	124:135	arg1	deconstruction					153:166	an experimental and theoretical deconstruction	121:166	an experimental and theoretical deconstruction of coulombic repulsion	121:189	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	9	56	theme	Molecular	1551:1559	arg1	MD					1571:1572	MD	1571:1572	MD	1571:1572	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	56	theme	Molecular	1551:1559	arg1	dynamics					1561:1568	Molecular dynamics	1551:1568	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides	1551:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	57	theme	theoretical	141:151	arg1	deconstruction					153:166	an experimental and theoretical deconstruction	121:166	an experimental and theoretical deconstruction of coulombic repulsion	121:189	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	3	58	theme	glycoproteomic	605:618	arg1	analyses					620:627	glycoproteomic analyses	605:627	glycoproteomic analyses	605:627	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	1	59	theme	mobility	225:232	arg1	IMS					248:250	IMS	248:250	IMS	248:250	Ion mobility spectrometry (IMS) is of significant interest as a platform for glycoanalysis.					
31453603	1	59	theme	mobility	225:232	arg1	spectrometry					234:245	Ion mobility spectrometry	221:245	Ion mobility spectrometry (IMS)	221:251	Ion mobility spectrometry (IMS) is of significant interest as a platform for glycoanalysis.					
31453603	10	60	theme	glycopeptide-enriched	2088:2108	arg1	regions					2110:2116	glycopeptide-enriched regions	2088:2116	glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics	2088:2179	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	2	61	theme	mobility	539:546	arg1	space					548:552	mobility space	539:552	mobility space	539:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	9	62	theme	compact	1971:1977	arg1	structures					1979:1988	more compact structures	1966:1988	more compact structures	1966:1988	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	5	63	theme	ions	947:950	arg1	study					908:912	an IMS study	901:912	an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	901:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	9	64	theme	dependent	1746:1754	arg1	interplay					1756:1764	charge state dependent interplay	1733:1764	charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures)	1733:1989	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	8	65	theme	ion	1506:1508	arg1	state					1517:1521	the ion charge state	1502:1521	the ion charge state	1502:1521	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	9	66	from	result	1956:1961	arg1	structures					1979:1988	more compact structures	1966:1988	more compact structures	1966:1988	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	0	67	theme	repulsion	181:189	arg1	deconstruction					153:166	an experimental and theoretical deconstruction	121:166	an experimental and theoretical deconstruction of coulombic repulsion	121:189	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	3	68	theme	glycosylated	633:644	arg1	peptides					667:674	glycosylated and non-glycosylated peptides	633:674	glycosylated and non-glycosylated peptides	633:674	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	9	69	gly	glycopeptides	1636:1648	arg2	glycopeptides					1636:1648	selected glycopeptides	1627:1648	selected glycopeptides	1627:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	6	70	theme	ion-neutral	1066:1076	arg1	sections					1094:1101	over 110 ion-neutral collision cross sections	1057:1101	over 110 ion-neutral collision cross sections (CCSs)	1057:1108	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	6	70	theme	ion-neutral	1066:1076	arg1	CCSs					1104:1107	CCSs	1104:1107	CCSs	1104:1107	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	4	71	theme	physical	739:746	arg1	characteristics					761:775	the physical and chemical characteristics	735:775	the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci	735:834	Nevertheless, the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci have yet to be systematically investigated.					
31453603	10	72	theme	MD	2022:2023	arg1	findings					2025:2032	these IMS and MD findings	2008:2032	these IMS and MD findings	2008:2032	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	0	73	theme	cross	68:72	arg1	sections					74:81	the ion-neutral collision cross sections	42:81	the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects	42:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	9	74	theme	coulombic	1788:1796	arg1	effects					1808:1814	coulombic repulsion effects	1788:1814	coulombic repulsion effects (tending to result in more extended structures)	1788:1862	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	10	75	theme	IMS	2014:2016	arg1	findings					2025:2032	these IMS and MD findings	2008:2032	these IMS and MD findings	2008:2032	Taken together, these IMS and MD findings suggest the possibility of predicting and delineating glycopeptide-enriched regions of mass vs. mobility space for applications in glycoproteomics.					
31453603	7	76	theme	decisive	1249:1256	arg1	factor					1258:1263	a decisive factor	1247:1263	a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion	1247:1383	Glycan size and composition emerged as a decisive factor in dictating the CCS space occupied by the glycopeptides and exerted this influence in a charge state dependent fashion.					
31453603	5	77	theme	protonated	923:932	arg1	ions					947:950	model protonated glycopeptide ions	917:950	model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states	917:1046	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	0	78	theme	N-linked	97:104	arg1	glycopeptides					106:118	protonated N-linked glycopeptides	86:118	protonated N-linked glycopeptides	86:118	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	9	79	theme	dynamics	1561:1568	arg1	simulations					1575:1585	Molecular dynamics (MD) simulations	1551:1585	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides	1551:1648	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	6	80	theme	characteristics	1175:1189	arg1	context					1144:1150	the context	1140:1150	the context of the physicochemical characteristics of the analytes	1140:1205	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	2	81	theme	mass	530:533	arg1	regions					519:525	distinct regions	510:525	distinct regions of mass vs. mobility space	510:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	8	82	theme	glycan	1543:1548	arg1	size					1531:1534	the size	1527:1534	the size of the glycan	1527:1548	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	8	82	theme	glycan	1543:1548	arg1	state					1517:1521	the ion charge state	1502:1521	the ion charge state	1502:1521	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	2	83	theme	glycoconjugates	399:413	arg1	resolution					358:367	the resolution	354:367	the resolution of isomeric carbohydrates and glycoconjugates	354:413	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	5	84	theme	oligosaccharide	979:993	arg1	topologies					995:1004	systematically varied oligosaccharide topologies	957:1004	systematically varied oligosaccharide topologies	957:1004	This report presents an IMS study of model protonated glycopeptide ions with systematically varied oligosaccharide topologies, polypeptide sequences, and charge states.					
31453603	3	85	contain	has	571:573	arg2	potential					575:583	potential	575:583	potential to greatly simplify glycoproteomic analyses	575:627	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	3	85	contain	has	571:573	arg1	capability					560:569	This capability	555:569	This capability	555:569	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	4	86	theme	chemical	752:759	arg1	characteristics					761:775	the physical and chemical characteristics	735:775	the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci	735:834	Nevertheless, the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci have yet to be systematically investigated.					
31453603	2	87	theme	carbohydrates	381:393	arg1	resolution					358:367	the resolution	354:367	the resolution of isomeric carbohydrates and glycoconjugates	354:413	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	0	88	gly	glycopeptides	106:118	arg2	glycopeptides					106:118	protonated N-linked glycopeptides	86:118	protonated N-linked glycopeptides	86:118	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	2	89	theme	IMS	444:446	arg1	ability					455:461	the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space	451:552	the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space	451:552	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	2	89	theme	IMS	444:446	arg1	aspect					434:439	another appealing aspect	416:439	another appealing aspect of IMS	416:446	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	9	90	from	result	1828:1833	arg1	structures					1852:1861	more extended structures	1838:1861	more extended structures	1838:1861	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	91	theme	phase	1598:1602	arg1	structures					1604:1613	gas phase structures	1594:1613	gas phase structures	1594:1613	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	6	92	theme	cross	1088:1092	arg1	sections					1094:1101	over 110 ion-neutral collision cross sections	1057:1101	over 110 ion-neutral collision cross sections (CCSs)	1057:1108	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	6	92	theme	cross	1088:1092	arg1	CCSs					1104:1107	CCSs	1104:1107	CCSs	1104:1107	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	2	93	theme	different	471:479	arg1	classes					481:487	different classes	471:487	different classes of biomolecules	471:503	While much attention has been focused on the resolution of isomeric carbohydrates and glycoconjugates, another appealing aspect of IMS is the ability to sort different classes of biomolecules into distinct regions of mass vs. mobility space.					
31453603	3	94	theme	non-glycosylated	650:665	arg1	peptides					667:674	glycosylated and non-glycosylated peptides	633:674	glycosylated and non-glycosylated peptides	633:674	This capability has potential to greatly simplify glycoproteomic analyses, as glycosylated and non-glycosylated peptides can be rapidly partitioned in the gas phase.					
31453603	8	95	theme	group	1424:1428	arg1	elongation					1399:1408	elongation	1399:1408	elongation of the glycan group	1399:1428	Furthermore, elongation of the glycan group was found to either increase or decrease glycopeptide CCSs depending on the ion charge state and the size of the glycan.					
31453603	4	96	gly	glycopeptides	780:792	arg2	glycopeptides					780:792	glycopeptides	780:792	glycopeptides that dictate their mass vs. mobility loci	780:834	Nevertheless, the physical and chemical characteristics of glycopeptides that dictate their mass vs. mobility loci have yet to be systematically investigated.					
31453603	0	97	theme	ion-neutral	46:56	arg1	sections					74:81	the ion-neutral collision cross sections	42:81	the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects	42:218	Composition and charge state influence on the ion-neutral collision cross sections of protonated N-linked glycopeptides: an experimental and theoretical deconstruction of coulombic repulsion vs. charge solvation effects.					
31453603	6	98	theme	physicochemical	1159:1173	arg1	characteristics					1175:1189	the physicochemical characteristics	1155:1189	the physicochemical characteristics of the analytes	1155:1205	In all, over 110 ion-neutral collision cross sections (CCSs) were measured and analyzed in the context of the physicochemical characteristics of the analytes.					
31453603	9	99	theme	charge	1880:1885	arg1	effects					1897:1903	charge solvation effects	1880:1903	charge solvation effects in which the glycan plays a major role (tending to result in more compact structures)	1880:1989	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	100	with	consistent	1699:1708	arg1	interplay					1756:1764	charge state dependent interplay	1733:1764	charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures)	1733:1989	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	100	with	consistent	1699:1708	arg1	size					1724:1727	a glycan size	1715:1727	a glycan size	1715:1727	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	101	theme	glycopeptides	1636:1648	arg1	CCSs					1619:1622	CCSs	1619:1622	CCSs	1619:1622	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31453603	9	101	theme	glycopeptides	1636:1648	arg1	structures					1604:1613	gas phase structures	1594:1613	gas phase structures	1594:1613	Molecular dynamics (MD) simulations of the gas phase structures and CCSs of selected glycopeptides revealed that the experimental observations were consistent with a glycan size and charge state dependent interplay between destabilizing coulombic repulsion effects (tending to result in more extended structures) and stabilizing charge solvation effects in which the glycan plays a major role (tending to result in more compact structures).					
31507595	2	0	theme	T	325:325	arg1	cells					327:331	activated T cells	315:331	activated T cells	315:331	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	1	1	theme	several	212:218	arg1	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	9	2	theme	type-2	1653:1658	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	3	3	theme	group	477:481	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	1	4	theme	O-glycosylation	230:244	arg1	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	5	5	gly	sialylated	829:838	arg1	N-glycans					840:848	N-glycosylation and multi-antennary sialylated N-glycans	793:848	N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	793:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	1	6	gly	glycosylation	166:178	arg2	asparagine					188:197	asparagine 3	188:199	asparagine 3 (Asn3)	188:206	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	6	gly	glycosylation	166:178	arg2	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	6	gly	glycosylation	166:178	arg2	Asn3					202:205	Asn3	202:205	Asn3	202:205	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	6	gly	glycosylation	166:178	arg2	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	6	gly	glycosylation	166:178	arg2	one					153:155	one	153:155	one	153:155	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	0	7	theme	Specific	0:7	arg1	Sialoforms					9:18	Specific Sialoforms	0:18	Specific Sialoforms	0:18	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	5	8	theme	polyLacNAc	864:873	arg1	extensions					875:884	abundant polyLacNAc extensions	855:884	abundant polyLacNAc extensions	855:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	1	9	from	sites	263:267	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	10	theme	N-linked	157:164	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	9	11	from	addition	1629:1636	arg1	abundant					1704:1711	abundant	1704:1711	abundant	1704:1711	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	6	12	theme	sialylated	1206:1215	arg1	N-glycans					1217:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	6	13	gly	sialylated	1206:1215	arg1	N-glycans					1217:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	3	14	theme	Box	483:485	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	15	theme	sialylated	829:838	arg1	N-glycans					840:848	N-glycosylation and multi-antennary sialylated N-glycans	793:848	N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	793:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	1	16	theme	small	104:108	arg1	glycopeptide					110:121	a small glycopeptide	102:121	a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	102:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	16	theme	small	104:108	arg1	CD52					94:97	Human CD52	88:97	Human CD52	88:97	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	16	theme	small	104:108	arg1	residues					138:145	12 amino acid residues	124:145	12 amino acid residues	124:145	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	6	17	dep	Glycomic	955:962	arg1	C8-LC-ESI-MS					1020:1031	C8-LC-ESI-MS	1020:1031	C8-LC-ESI-MS	1020:1031	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	6	17	dep	Glycomic	955:962	arg1	carbon-ESI-MS/MS					984:999	porous graphitized carbon-ESI-MS/MS	965:999	porous graphitized carbon-ESI-MS/MS	965:999	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	2	18	theme	immune	346:351	arg1	suppression					353:363	immune suppression	346:363	immune suppression	346:363	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	8	19	theme	CD52-Fc	1367:1373	arg1	glycoforms					1353:1362	glycoforms	1353:1362	glycoforms of CD52-Fc	1353:1373	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	1	20	gly	glycopeptide	110:121	arg2	residues					138:145	12 amino acid residues	124:145	12 amino acid residues	124:145	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	20	gly	glycopeptide	110:121	arg2	CD52					94:97	Human CD52	88:97	Human CD52	88:97	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	20	gly	glycopeptide	110:121	arg2	glycopeptide					110:121	a small glycopeptide	102:121	a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	102:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	3	21	theme	sialic	554:559	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	6	22	theme	porous	965:970	arg1	carbon-ESI-MS/MS					984:999	porous graphitized carbon-ESI-MS/MS	965:999	porous graphitized carbon-ESI-MS/MS	965:999	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	8	23	theme	structures	1578:1587	arg1	abundance					1548:1556	a lower relative abundance	1531:1556	a lower relative abundance of bisecting GlcNAc structures	1531:1587	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	8	23	theme	structures	1578:1587	arg1	structures					1516:1525	sialylated N-glycan structures	1496:1525	sialylated N-glycan structures	1496:1525	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	10	24	theme	immunotherapeutic	1855:1871	arg1	development					1837:1847	its development	1833:1847	its development	1833:1847	Understanding the structural features of CD52 glycan required for its bioactivity will aid its development as an immunotherapeutic agent.					
31507595	10	24	theme	immunotherapeutic	1855:1871	arg1	agent					1873:1877	an immunotherapeutic agent	1852:1877	an immunotherapeutic agent	1852:1877	Understanding the structural features of CD52 glycan required for its bioactivity will aid its development as an immunotherapeutic agent.					
31507595	10	25	theme	structural	1760:1769	arg1	features					1771:1778	the structural features	1756:1778	the structural features of CD52 glycan required for its bioactivity	1756:1822	Understanding the structural features of CD52 glycan required for its bioactivity will aid its development as an immunotherapeutic agent.					
31507595	5	26	theme	type	938:941	arg1	structures					943:952	mainly di-sialylated O-glycosylation type structures	901:952	mainly di-sialylated O-glycosylation type structures	901:952	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	6	27	theme	Glycomic	955:962	arg1	analysis					1034:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis	955:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins	955:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	5	28	gly	di-sialylated	908:920	arg1	structures					943:952	mainly di-sialylated O-glycosylation type structures	901:952	mainly di-sialylated O-glycosylation type structures	901:952	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	8	29	theme	anion	1396:1400	arg1	chromatography					1420:1433	anion exchange MonoQ-GL chromatography	1396:1433	anion exchange MonoQ-GL chromatography	1396:1433	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	9	30	theme	O-glycan	1639:1646	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	9	31	from	abundant	1704:1711	arg1	fractions					1731:1739	bioactive CD52 fractions	1716:1739	bioactive CD52 fractions	1716:1739	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	9	31	from	abundant	1704:1711	arg1	addition					1629:1636	addition	1629:1636	addition	1629:1636	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	6	32	theme	soluble	1058:1064	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	8	33	theme	MonoQ-GL	1411:1418	arg1	chromatography					1420:1433	anion exchange MonoQ-GL chromatography	1396:1433	anion exchange MonoQ-GL chromatography	1396:1433	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	5	34	theme	CD52	725:728	arg1	Analysis					706:713	Analysis	706:713	Analysis of native CD52 purified from human spleen	706:755	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	3	35	theme	immunoglobulin-like	574:592	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	1	36	theme	amino	127:131	arg1	glycopeptide					110:121	a small glycopeptide	102:121	a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	102:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	36	theme	amino	127:131	arg1	residues					138:145	12 amino acid residues	124:145	12 amino acid residues	124:145	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	4	37	theme	CD52	663:666	arg1	glycan					668:673	CD52 glycan	663:673	CD52 glycan	663:673	We aimed to identify the features of CD52 glycan that underlie its bioactivity.					
31507595	0	38	theme	Soluble	74:80	arg1	CD52					82:85	Human Soluble CD52	68:85	Human Soluble CD52	68:85	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	5	39	theme	di-sialylated	908:920	arg1	structures					943:952	mainly di-sialylated O-glycosylation type structures	901:952	mainly di-sialylated O-glycosylation type structures	901:952	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	3	40	theme	T	413:413	arg1	cells					415:419	activated T cells	403:419	activated T cells	403:419	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	41	from	heterogeneity	776:788	arg1	N-glycans					840:848	N-glycosylation and multi-antennary sialylated N-glycans	793:848	N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	793:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	0	42	theme	Immune	37:42	arg1	Activity					56:63	the Immune Suppressive Activity	33:63	the Immune Suppressive Activity of Human Soluble CD52	33:85	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	6	43	theme	CD52	1125:1128	arg1	bioactivity					1130:1140	CD52 bioactivity	1125:1140	CD52 bioactivity	1125:1140	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	9	44	gly	di-sialylated	1660:1672	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	0	45	theme	Human	68:72	arg1	CD52					82:85	Human Soluble CD52	68:85	Human Soluble CD52	68:85	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	7	46	theme	sialylation	1245:1255	arg1	Removal					1228:1234	Removal	1228:1234	Removal of α-2,3 sialylation	1228:1255	Removal of α-2,3 sialylation abolished bioactivity, which was restored by re-sialylation with α-2,3 sialyltransferases.					
31507595	1	47	theme	serine/threonine	246:261	arg1	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	48	gly	O-glycosylation	230:244	arg2	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	49	theme	Human	88:92	arg1	glycopeptide					110:121	a small glycopeptide	102:121	a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	102:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	49	theme	Human	88:92	arg1	CD52					94:97	Human CD52	88:97	Human CD52	88:97	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	5	50	with	N-glycans	840:848	arg1	extensions					875:884	abundant polyLacNAc extensions	855:884	abundant polyLacNAc extensions	855:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	8	51	theme	sialylated	1496:1505	arg1	structures					1516:1525	sialylated N-glycan structures	1496:1525	sialylated N-glycan structures	1496:1525	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	9	52	theme	CD52	1726:1729	arg1	fractions					1731:1739	bioactive CD52 fractions	1716:1739	bioactive CD52 fractions	1716:1739	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	9	53	from	fractions	1731:1739	arg1	abundant					1704:1711	abundant	1704:1711	abundant	1704:1711	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	6	54	theme	fusion	1095:1100	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	6	55	theme	CD52-immunoglobulin	1072:1090	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	3	56	theme	high	463:466	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	57	theme	extensive	766:774	arg1	heterogeneity					776:788	extensive heterogeneity	766:788	extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	766:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	8	58	theme	relative	1539:1546	arg1	abundance					1548:1556	a lower relative abundance	1531:1556	a lower relative abundance of bisecting GlcNAc structures	1531:1587	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	6	59	theme	high	1164:1167	arg1	abundance					1169:1177	a high abundance	1162:1177	a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans	1162:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	2	60	theme	cells	327:331	arg1	surface					304:310	the surface	300:310	the surface of activated T cells	300:331	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	8	61	theme	bisecting	1561:1569	arg1	structures					1578:1587	bisecting GlcNAc structures	1561:1587	bisecting GlcNAc structures	1561:1587	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	7	62	theme	α-2,3	1322:1326	arg1	sialyltransferases					1328:1345	α-2,3 sialyltransferases	1322:1345	α-2,3 sialyltransferases	1322:1345	Removal of α-2,3 sialylation abolished bioactivity, which was restored by re-sialylation with α-2,3 sialyltransferases.					
31507595	2	63	theme	Soluble	270:276	arg1	CD52					278:281	Soluble CD52	270:281	Soluble CD52	270:281	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	1	64	theme	potential	220:228	arg1	sites					263:267	several potential O-glycosylation serine/threonine sites	212:267	several potential O-glycosylation serine/threonine sites	212:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	9	65	theme	di-sialylated	1660:1672	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	2	66	theme	activated	315:323	arg1	cells					327:331	activated T cells	315:331	activated T cells	315:331	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	3	67	theme	HMGB1	490:494	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	68	theme	abundant	855:862	arg1	extensions					875:884	abundant polyLacNAc extensions	855:884	abundant polyLacNAc extensions	855:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	0	69	theme	Suppressive	44:54	arg1	Activity					56:63	the Immune Suppressive Activity	33:63	the Immune Suppressive Activity of Human Soluble CD52	33:85	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	6	70	gly	glycopeptide	1006:1017	arg2	glycopeptide					1006:1017	glycopeptide	1006:1017	glycopeptide	1006:1017	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	2	71	attach	released	286:293	arg2	CD52					278:281	Soluble CD52	270:281	Soluble CD52	270:281	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	2	71	attach	released	286:293	arg1	surface					304:310	the surface	300:310	the surface of activated T cells	300:331	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	6	72	theme	N-glycans	1217:1225	arg1	abundance					1169:1177	a high abundance	1162:1177	a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans	1162:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	1	73	theme	glycosylation	166:178	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	8	74	theme	GlcNAc	1571:1576	arg1	structures					1578:1587	bisecting GlcNAc structures	1561:1587	bisecting GlcNAc structures	1561:1587	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	6	75	theme	α-2,3/6	1198:1204	arg1	N-glycans					1217:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	10	76	theme	CD52	1783:1786	arg1	glycan					1788:1793	CD52 glycan	1783:1793	CD52 glycan required for its bioactivity	1783:1822	Understanding the structural features of CD52 glycan required for its bioactivity will aid its development as an immunotherapeutic agent.					
31507595	3	77	theme	inhibitory	543:552	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	8	78	dep	tetra-antennary	1473:1487	arg1	α-2,3					1490:1494	α-2,3	1490:1494	α-2,3	1490:1494	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	6	79	theme	graphitized	972:982	arg1	carbon-ESI-MS/MS					984:999	porous graphitized carbon-ESI-MS/MS	965:999	porous graphitized carbon-ESI-MS/MS	965:999	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	10	80	theme	glycan	1788:1793	arg1	features					1771:1778	the structural features	1756:1778	the structural features of CD52 glycan required for its bioactivity	1756:1822	Understanding the structural features of CD52 glycan required for its bioactivity will aid its development as an immunotherapeutic agent.					
31507595	1	81	from	asparagine	188:197	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	5	82	theme	O-glycosylation	922:936	arg1	structures					943:952	mainly di-sialylated O-glycosylation type structures	901:952	mainly di-sialylated O-glycosylation type structures	901:952	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	8	83	theme	non-bioactive	1601:1613	arg1	fractions					1615:1623	non-bioactive fractions	1601:1623	non-bioactive fractions	1601:1623	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	4	84	theme	glycan	668:673	arg1	features					651:658	the features	647:658	the features of CD52 glycan that underlie its bioactivity	647:703	We aimed to identify the features of CD52 glycan that underlie its bioactivity.					
31507595	8	85	gly	glycoforms	1353:1362	arg1	CD52-Fc					1367:1373	CD52-Fc	1367:1373	CD52-Fc	1367:1373	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	3	86	theme	lectin-10	594:602	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	6	87	theme	human	1066:1070	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	5	88	theme	native	718:723	arg1	CD52					725:728	native CD52	718:728	native CD52 purified from human spleen	718:755	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	3	89	theme	acid-binding	561:572	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	90	theme	multi-antennary	813:827	arg1	N-glycans					840:848	N-glycosylation and multi-antennary sialylated N-glycans	793:848	N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	793:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	9	91	theme	core	1648:1651	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	6	92	theme	recombinant	1046:1056	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	7	93	with	re-sialylation	1302:1315	arg1	sialyltransferases					1328:1345	α-2,3 sialyltransferases	1322:1345	α-2,3 sialyltransferases	1322:1345	Removal of α-2,3 sialylation abolished bioactivity, which was restored by re-sialylation with α-2,3 sialyltransferases.					
31507595	8	94	theme	exchange	1402:1409	arg1	chromatography					1420:1433	anion exchange MonoQ-GL chromatography	1396:1433	anion exchange MonoQ-GL chromatography	1396:1433	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	0	95	theme	CD52	82:85	arg1	Activity					56:63	the Immune Suppressive Activity	33:63	the Immune Suppressive Activity of Human Soluble CD52	33:85	Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52.					
31507595	1	96	theme	acid	133:136	arg1	glycopeptide					110:121	a small glycopeptide	102:121	a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	102:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	96	theme	acid	133:136	arg1	residues					138:145	12 amino acid residues	124:145	12 amino acid residues	124:145	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	6	97	theme	glycopeptide	1006:1017	arg1	analysis					1034:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis	955:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins	955:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	9	98	from	Ser12	1688:1692	arg1	structures					1674:1683	O-glycan core type-2 di-sialylated structures	1639:1683	O-glycan core type-2 di-sialylated structures at Ser12	1639:1692	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	8	99	theme	bioactive	1436:1444	arg1	fractions					1446:1454	bioactive fractions	1436:1454	bioactive fractions	1436:1454	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	1	100	with	glycopeptide	110:121	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	1	101	link	N-linked	157:164	arg1	site					180:183	one N-linked glycosylation site	153:183	one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites	153:267	Human CD52 is a small glycopeptide (12 amino acid residues) with one N-linked glycosylation site at asparagine 3 (Asn3) and several potential O-glycosylation serine/threonine sites.					
31507595	7	102	theme	α-2,3	1239:1243	arg1	sialylation					1245:1255	α-2,3 sialylation	1239:1255	α-2,3 sialylation	1239:1255	Removal of α-2,3 sialylation abolished bioactivity, which was restored by re-sialylation with α-2,3 sialyltransferases.					
31507595	8	103	theme	tetra-antennary	1473:1487	arg1	structures					1516:1525	sialylated N-glycan structures	1496:1525	sialylated N-glycan structures	1496:1525	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	3	104	theme	activated	403:411	arg1	cells					415:419	activated T cells	403:419	activated T cells	403:419	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	5	105	theme	N-glycosylation	793:807	arg1	N-glycans					840:848	N-glycosylation and multi-antennary sialylated N-glycans	793:848	N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions	793:884	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	9	106	theme	bioactive	1716:1724	arg1	fractions					1731:1739	bioactive CD52 fractions	1716:1739	bioactive CD52 fractions	1716:1739	In addition, O-glycan core type-2 di-sialylated structures at Ser12 were more abundant in bioactive CD52 fractions.					
31507595	6	107	theme	proteins	1102:1109	arg1	analysis					1034:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis	955:1041	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins	955:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	5	108	theme	human	744:748	arg1	spleen					750:755	human spleen	744:755	human spleen	744:755	Analysis of native CD52 purified from human spleen revealed extensive heterogeneity in N-glycosylation and multi-antennary sialylated N-glycans with abundant polyLacNAc extensions, together with mainly di-sialylated O-glycosylation type structures.					
31507595	6	109	theme	Fc	1092:1093	arg1	proteins					1102:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	recombinant soluble human CD52-immunoglobulin Fc fusion proteins	1046:1109	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	3	110	theme	Siglec-10	605:613	arg1	receptor					616:623	inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	543:623	the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor	539:623	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	8	111	theme	N-glycan	1507:1514	arg1	structures					1516:1525	sialylated N-glycan structures	1496:1525	sialylated N-glycan structures	1496:1525	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	2	112	theme	glycan	373:378	arg1	moiety					380:385	its glycan moiety	369:385	its glycan moiety	369:385	Soluble CD52 is released from the surface of activated T cells and mediates immune suppression via its glycan moiety.					
31507595	6	113	theme	tetra-antennary	1182:1196	arg1	N-glycans					1217:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	tetra-antennary α-2,3/6 sialylated N-glycans	1182:1225	Glycomic (porous graphitized carbon-ESI-MS/MS) and glycopeptide (C8-LC-ESI-MS) analysis of recombinant soluble human CD52-immunoglobulin Fc fusion proteins revealed that CD52 bioactivity was correlated with a high abundance of tetra-antennary α-2,3/6 sialylated N-glycans.					
31507595	3	114	theme	mobility	468:475	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31507595	8	115	gly	sialylated	1496:1505	arg1	structures					1516:1525	sialylated N-glycan structures	1496:1525	sialylated N-glycan structures	1496:1525	When glycoforms of CD52-Fc were fractionated by anion exchange MonoQ-GL chromatography, bioactive fractions displayed mainly tetra-antennary, α-2,3 sialylated N-glycan structures and a lower relative abundance of bisecting GlcNAc structures compared to non-bioactive fractions.					
31507595	3	116	theme	pro-inflammatory	446:461	arg1	protein					497:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	the pro-inflammatory high mobility group Box 1 (HMGB1) protein	442:503	In suppressing activated T cells, it first sequesters the pro-inflammatory high mobility group Box 1 (HMGB1) protein, which facilitates its binding to the inhibitory sialic acid-binding immunoglobulin-like lectin-10 (Siglec-10) receptor.					
31362986	6	0	theme	isothermal	1039:1048	arg1	calorimetry					1060:1070	differential scanning and isothermal titration calorimetry	1013:1070	calorimetry	1060:1070	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	6	1	theme	complex	1280:1286	arg1	formation					1288:1296	complex formation	1280:1296	complex formation	1280:1296	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	3	2	theme	ΔXT/FT	566:571	arg1	plants					573:578	Nicotiana benthamiana ΔXT/FT plants	544:578	Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	544:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	3	3	theme	human	437:441	arg1	receptor					467:474	human epidermal growth factor receptor 2	437:476	human epidermal growth factor receptor 2	437:476	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	7	4	theme	cognate	1415:1421	arg1	ligand					1423:1428	its cognate ligand	1411:1428	its cognate ligand	1411:1428	Our results suggest that different N-glycans on the receptor significantly contribute to binding affinities for its cognate ligand.					
31362986	4	5	theme	distinct	751:758	arg1	N-glycans					771:779	distinct homogenous N-glycans	751:779	distinct homogenous N-glycans	751:779	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	6	6	theme	FcαRI	1211:1215	arg1	glycoforms					1217:1226	four different FcαRI glycoforms	1196:1226	four different FcαRI glycoforms	1196:1226	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	1	7	theme	prevalent	141:149	arg1	A					121:121	Human immunoglobulin A	100:121	Human immunoglobulin A (IgA)	100:127	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	7	theme	prevalent	141:149	arg1	class					160:164	the most prevalent antibody class	132:164	the most prevalent antibody class at mucosal sites with an important role in mucosal defense	132:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	7	8	theme	binding	1388:1394	arg1	affinities					1396:1405	binding affinities	1388:1405	binding affinities for its cognate ligand	1388:1428	Our results suggest that different N-glycans on the receptor significantly contribute to binding affinities for its cognate ligand.					
31362986	2	9	theme	N-glycan	262:269	arg1	modifications					271:283	N-glycan modifications	262:283	N-glycan modifications of IgA1 and IgA2	262:300	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	3	10	theme	nonhuman	627:634	arg1	structures					645:654	nonhuman N-glycan structures	627:654	nonhuman N-glycan structures	627:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	1	11	theme	mucosal	169:175	arg1	sites					177:181	mucosal sites	169:181	mucosal sites	169:181	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	12	theme	immunoglobulin	106:119	arg1	A					121:121	Human immunoglobulin A	100:121	Human immunoglobulin A (IgA)	100:127	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	12	theme	immunoglobulin	106:119	arg1	class					160:164	the most prevalent antibody class	132:164	the most prevalent antibody class at mucosal sites with an important role in mucosal defense	132:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	12	theme	immunoglobulin	106:119	arg1	IgA					124:126	IgA	124:126	IgA	124:126	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	6	13	theme	formation	1288:1296	arg1	kinetics					1268:1275	kinetics	1268:1275	kinetics	1268:1275	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	6	13	theme	formation	1288:1296	arg1	thermodynamics					1249:1262	the thermodynamics	1245:1262	the thermodynamics	1245:1262	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	1	14	from	sites	177:181	arg1	A					121:121	Human immunoglobulin A	100:121	Human immunoglobulin A (IgA)	100:127	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	14	from	sites	177:181	arg1	class					160:164	the most prevalent antibody class	132:164	the most prevalent antibody class at mucosal sites with an important role in mucosal defense	132:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	6	15	gly	glycoforms	1217:1226	arg1	FcαRI					1211:1215	four different FcαRI glycoforms	1196:1226	four different FcαRI glycoforms	1196:1226	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	9	16	theme	human	1702:1706	arg1	IgA					1708:1710	human IgA	1702:1710	human IgA	1702:1710	In conclusion, N-glycans of human IgA do not affect its structure and integrity but its thermal stability, and FcαRI N-glycans significantly modulate binding affinity to IgA.					
31362986	7	17	theme	different	1324:1332	arg1	N-glycans					1334:1342	different N-glycans	1324:1342	different N-glycans on the receptor	1324:1358	Our results suggest that different N-glycans on the receptor significantly contribute to binding affinities for its cognate ligand.					
31362986	6	18	theme	IgA2	1165:1168	arg1	glycoforms					1170:1179	distinct IgA1 and IgA2 glycoforms	1147:1179	distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms	1147:1226	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	5	19	dep	structure	890:898	arg1	the					878:880	the	878:880	the	878:880	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	5	20	theme	antigen	859:865	arg1	binding					867:873	antigen binding	859:873	antigen binding	859:873	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	4	21	contain	carrying	742:749	arg2	N-glycans					771:779	distinct homogenous N-glycans	751:779	distinct homogenous N-glycans	751:779	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	4	21	contain	carrying	742:749	arg1	IgA					738:740	IgA	738:740	IgA carrying distinct homogenous N-glycans	738:779	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	6	22	theme	IgA1	1156:1159	arg1	glycoforms					1170:1179	distinct IgA1 and IgA2 glycoforms	1147:1179	distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms	1147:1226	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	5	23	theme	different	813:821	arg1	profiles					832:839	distinctly different N-glycan profiles	802:839	distinctly different N-glycan profiles	802:839	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	0	24	theme	A	80:80	arg1	antibodies					88:97	immunoglobulin A (IgA) antibodies	65:97	immunoglobulin A (IgA) antibodies	65:97	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	6	25	theme	differential	1013:1024	arg1	scanning					1026:1033	differential scanning and isothermal titration calorimetry	1013:1070	scanning	1026:1033	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	0	26	theme	Distinct	0:7	arg1	N-glycans					22:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	8	27	theme	complexes	1506:1514	arg1	mixture					1483:1489	a mixture	1481:1489	a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it	1481:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	0	28	theme	receptor	13:20	arg1	N-glycans					22:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	5	29	theme	overall	882:888	arg1	structure					890:898	overall structure	882:898	overall structure	882:898	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	2	30	theme	extracellular	384:396	arg1	domain					398:403	its extracellular domain	380:403	its extracellular domain	380:403	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	8	31	theme	1:1	1533:1535	arg1	stoichiometry					1537:1549	a 1:1 stoichiometry	1531:1549	a 1:1 stoichiometry due to different IgA tailpiece conformations that make it	1531:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	2	32	theme	IgA2	297:300	arg1	modifications					271:283	N-glycan modifications	262:283	N-glycan modifications of IgA1 and IgA2	262:300	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	0	33	theme	binding	45:51	arg1	affinity					53:60	the binding affinity	41:60	the binding affinity to immunoglobulin A (IgA) antibodies	41:97	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	1	34	with	class	160:164	arg1	role					201:204	an important role	188:204	an important role in mucosal defense	188:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	5	35	theme	thermal	957:963	arg1	stability					965:973	their thermal stability	951:973	their thermal stability	951:973	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	6	36	theme	plasmon	1081:1087	arg1	resonance					1089:1097	surface plasmon resonance	1073:1097	surface plasmon resonance spectroscopy	1073:1110	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	8	37	theme	due	1551:1553	arg1	stoichiometry					1537:1549	a 1:1 stoichiometry	1531:1549	a 1:1 stoichiometry due to different IgA tailpiece conformations that make it	1531:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	3	38	theme	benthamiana	554:564	arg1	plants					573:578	Nicotiana benthamiana ΔXT/FT plants	544:578	Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	544:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	4	39	theme	respective	688:697	arg1	enzymes					716:722	the respective glycan-modifying enzymes	684:722	the respective glycan-modifying enzymes	684:722	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	2	40	theme	IgA1	288:291	arg1	modifications					271:283	N-glycan modifications	262:283	N-glycan modifications of IgA1 and IgA2	262:300	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	5	41	theme	IgA	921:923	arg1	antibodies					925:934	the IgA antibodies	917:934	the IgA antibodies	917:934	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	8	42	theme	different	1558:1566	arg1	conformations					1582:1594	different IgA tailpiece conformations	1558:1594	different IgA tailpiece conformations that make it	1558:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	3	43	from	variants	532:539	arg1	plants					573:578	Nicotiana benthamiana ΔXT/FT plants	544:578	Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	544:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	8	44	used	occupied	1664:1671	arg2	sites					1639:1643	both binding sites	1626:1643	both binding sites	1626:1643	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	3	45	theme	epidermal	443:451	arg1	receptor					467:474	human epidermal growth factor receptor 2	437:476	human epidermal growth factor receptor 2	437:476	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	6	46	theme	titration	1050:1058	arg1	calorimetry					1060:1070	differential scanning and isothermal titration calorimetry	1013:1070	calorimetry	1060:1070	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	8	47	theme	tailpiece	1572:1580	arg1	conformations					1582:1594	different IgA tailpiece conformations	1558:1594	different IgA tailpiece conformations that make it	1558:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	1	48	theme	mucosal	209:215	arg1	defense					217:223	mucosal defense	209:223	mucosal defense	209:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	2	49	theme	modifications	271:283	arg1	impact					252:257	the impact	248:257	the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain	248:403	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	5	50	theme	N-glycan	823:830	arg1	profiles					832:839	distinctly different N-glycan profiles	802:839	distinctly different N-glycan profiles	802:839	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	6	51	gly	glycoforms	1170:1179	arg1	IgA2					1165:1168	IgA2	1165:1168	IgA2	1165:1168	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	6	51	gly	glycoforms	1170:1179	arg1	IgA1					1156:1159	distinct IgA1	1147:1159	distinct IgA1	1147:1159	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	3	52	theme	factor	460:465	arg1	receptor					467:474	human epidermal growth factor receptor 2	437:476	human epidermal growth factor receptor 2	437:476	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	3	53	theme	responsible	600:610	arg1	enzymes					592:598	the enzymes	588:598	the enzymes responsible for generating nonhuman N-glycan structures	588:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	6	54	theme	different	1201:1209	arg1	glycoforms					1217:1226	four different FcαRI glycoforms	1196:1226	four different FcαRI glycoforms	1196:1226	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	3	55	theme	-binding	484:491	arg1	IgA1					503:506	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1	437:506	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1	437:506	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	1	56	theme	antibody	151:158	arg1	A					121:121	Human immunoglobulin A	100:121	Human immunoglobulin A (IgA)	100:127	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	56	theme	antibody	151:158	arg1	class					160:164	the most prevalent antibody class	132:164	the most prevalent antibody class at mucosal sites with an important role in mucosal defense	132:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	4	57	theme	homogenous	760:769	arg1	N-glycans					771:779	distinct homogenous N-glycans	751:779	distinct homogenous N-glycans	751:779	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	3	58	theme	monomeric	493:501	arg1	IgA1					503:506	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1	437:506	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1	437:506	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	3	59	theme	N-glycan	636:643	arg1	structures					645:654	nonhuman N-glycan structures	627:654	nonhuman N-glycan structures	627:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	3	60	theme	IgA2m	509:513	arg1	variants					532:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants	437:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	437:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	1	61	from	role	201:204	arg1	defense					217:223	mucosal defense	209:223	mucosal defense	209:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	3	62	theme	IgA1	503:506	arg1	variants					532:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants	437:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	437:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	1	63	theme	Human	100:104	arg1	A					121:121	Human immunoglobulin A	100:121	Human immunoglobulin A (IgA)	100:127	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	63	theme	Human	100:104	arg1	class					160:164	the most prevalent antibody class	132:164	the most prevalent antibody class at mucosal sites with an important role in mucosal defense	132:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	1	63	theme	Human	100:104	arg1	IgA					124:126	IgA	124:126	IgA	124:126	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	8	64	theme	full-length	1450:1460	arg1	IgA					1462:1464	full-length IgA	1450:1464	full-length IgA	1450:1464	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	9	65	theme	IgA	1708:1710	arg1	N-glycans					1689:1697	N-glycans	1689:1697	N-glycans of human IgA	1689:1710	In conclusion, N-glycans of human IgA do not affect its structure and integrity but its thermal stability, and FcαRI N-glycans significantly modulate binding affinity to IgA.					
31362986	0	66	theme	immunoglobulin	65:78	arg1	A					80:80	immunoglobulin A	65:80	immunoglobulin A (IgA) antibodies	65:97	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	0	66	theme	immunoglobulin	65:78	arg1	IgA					83:85	IgA	83:85	IgA	83:85	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	6	67	theme	distinct	1147:1154	arg1	IgA1					1156:1159	distinct IgA1	1147:1159	distinct IgA1	1147:1159	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	3	68	dep	-binding	484:491	arg1	receptor					467:474	human epidermal growth factor receptor 2	437:476	human epidermal growth factor receptor 2	437:476	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	2	69	from	impact	252:257	arg1	binding					305:311	binding	305:311	binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain	305:403	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	6	70	theme	molecular	1117:1125	arg1	modeling					1127:1134	molecular modeling	1117:1134	molecular modeling	1117:1134	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	8	71	theme	1:1	1494:1496	arg1	complexes					1506:1514	1:1 and 1:2 complexes	1494:1514	1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it	1494:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	8	72	theme	1:2	1502:1504	arg1	complexes					1506:1514	1:1 and 1:2 complexes	1494:1514	1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it	1494:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	0	73	theme	Fcα	9:11	arg1	N-glycans					22:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans	0:30	Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.					
31362986	7	74	from	N-glycans	1334:1342	arg1	receptor					1351:1358	the receptor	1347:1358	the receptor	1347:1358	Our results suggest that different N-glycans on the receptor significantly contribute to binding affinities for its cognate ligand.					
31362986	9	75	theme	thermal	1762:1768	arg1	stability					1770:1778	its thermal stability	1758:1778	its thermal stability	1758:1778	In conclusion, N-glycans of human IgA do not affect its structure and integrity but its thermal stability, and FcαRI N-glycans significantly modulate binding affinity to IgA.					
31362986	2	76	theme	Fcα	320:322	arg1	FcαRI					334:338	FcαRI	334:338	FcαRI	334:338	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	2	76	theme	Fcα	320:322	arg1	receptor					324:331	the Fcα receptor	316:331	the Fcα receptor (FcαRI)	316:339	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	6	77	theme	size-exclusion	982:995	arg1	chromatography					997:1010	size-exclusion chromatography	982:1010	size-exclusion chromatography	982:1010	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	9	78	theme	FcαRI	1785:1789	arg1	N-glycans					1791:1799	FcαRI N-glycans	1785:1799	FcαRI N-glycans	1785:1799	In conclusion, N-glycans of human IgA do not affect its structure and integrity but its thermal stability, and FcαRI N-glycans significantly modulate binding affinity to IgA.					
31362986	3	79	theme	IgA2m	523:527	arg1	variants					532:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants	437:539	human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	437:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	6	80	theme	resonance	1089:1097	arg1	spectroscopy					1099:1110	surface plasmon resonance spectroscopy	1073:1110	surface plasmon resonance spectroscopy	1073:1110	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	3	81	theme	Nicotiana	544:552	arg1	plants					573:578	Nicotiana benthamiana ΔXT/FT plants	544:578	Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures	544:654	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
31362986	2	82	gly	glycosylated	364:375	arg2	domain					398:403	its extracellular domain	380:403	its extracellular domain	380:403	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	2	82	gly	glycosylated	364:375	arg1	FcαRI					334:338	FcαRI	334:338	FcαRI	334:338	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	2	82	gly	glycosylated	364:375	arg1	receptor					324:331	the Fcα receptor	316:331	the Fcα receptor (FcαRI)	316:339	Little is known about the impact of N-glycan modifications of IgA1 and IgA2 on binding to the Fcα receptor (FcαRI), which is also heavily glycosylated at its extracellular domain.					
31362986	6	83	theme	surface	1073:1079	arg1	resonance					1089:1097	surface plasmon resonance	1073:1097	surface plasmon resonance spectroscopy	1073:1110	Using size-exclusion chromatography, differential scanning and isothermal titration calorimetry, surface plasmon resonance spectroscopy, and molecular modeling, we probed distinct IgA1 and IgA2 glycoforms for binding to four different FcαRI glycoforms and investigated the thermodynamics and kinetics of complex formation.					
31362986	9	84	theme	binding	1824:1830	arg1	affinity					1832:1839	binding affinity	1824:1839	binding affinity to IgA	1824:1846	In conclusion, N-glycans of human IgA do not affect its structure and integrity but its thermal stability, and FcαRI N-glycans significantly modulate binding affinity to IgA.					
31362986	8	85	theme	IgA	1568:1570	arg1	conformations					1582:1594	different IgA tailpiece conformations	1558:1594	different IgA tailpiece conformations that make it	1558:1607	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	8	86	theme	binding	1631:1637	arg1	sites					1639:1643	both binding sites	1626:1643	both binding sites	1626:1643	We also noted that full-length IgA and FcαRI form a mixture of 1:1 and 1:2 complexes tending toward a 1:1 stoichiometry due to different IgA tailpiece conformations that make it less likely that both binding sites are simultaneously occupied.					
31362986	1	87	theme	important	191:199	arg1	role					201:204	an important role	188:204	an important role in mucosal defense	188:223	Human immunoglobulin A (IgA) is the most prevalent antibody class at mucosal sites with an important role in mucosal defense.					
31362986	5	88	theme	antibodies	925:934	arg1	binding					867:873	antigen binding	859:873	antigen binding	859:873	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	5	88	theme	antibodies	925:934	arg1	integrity					904:912	integrity	904:912	integrity	904:912	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	5	88	theme	antibodies	925:934	arg1	structure					890:898	overall structure	882:898	overall structure	882:898	We demonstrate that distinctly different N-glycan profiles did not influence antigen binding or the overall structure and integrity of the IgA antibodies but did affect their thermal stability.					
31362986	4	89	theme	glycan-modifying	699:714	arg1	enzymes					716:722	the respective glycan-modifying enzymes	684:722	the respective glycan-modifying enzymes	684:722	By coinfiltrating IgA with the respective glycan-modifying enzymes, we generated IgA carrying distinct homogenous N-glycans.					
31362986	3	90	theme	growth	453:458	arg1	receptor					467:474	human epidermal growth factor receptor 2	437:476	human epidermal growth factor receptor 2	437:476	Here, we transiently expressed human epidermal growth factor receptor 2 (HER2)-binding monomeric IgA1, IgA2m(1), and IgA2m(2) variants in Nicotiana benthamiana ΔXT/FT plants lacking the enzymes responsible for generating nonhuman N-glycan structures.					
29222836	7	0	theme	molecular	1208:1216	arg1	shape					1218:1222	a tadpole-like molecular shape	1193:1222	a tadpole-like molecular shape with a rigid linker connecting the CD and CBM	1193:1268	The SAXS data reveal a tadpole-like molecular shape with a rigid linker connecting the CD and CBM.					
29222836	3	1	theme	GH7-catalytic	576:588	arg1	CD					598:599	CD	598:599	CD	598:599	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	3	1	theme	GH7-catalytic	576:588	arg1	domain					590:595	a GH7-catalytic domain	574:595	a GH7-catalytic domain (CD)	574:600	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	12	2	theme	Protein	2124:2130	arg1	Bank					2137:2140	the Protein Data Bank	2120:2140	the Protein Data Bank with accession number 5W11	2120:2167	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	7	3	with	shape	1218:1222	arg1	linker					1237:1242	a rigid linker	1229:1242	a rigid linker connecting the CD and CBM	1229:1268	The SAXS data reveal a tadpole-like molecular shape with a rigid linker connecting the CD and CBM.					
29222836	1	4	theme	glycosidic	214:223	arg1	linkages					225:232	β-1,4 glycosidic linkages	208:232	β-1,4 glycosidic linkages	208:232	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	8	5	theme	catalytic	1324:1332	arg1	efficiency					1334:1343	higher catalytic efficiency	1317:1343	higher catalytic efficiency	1317:1343	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	2	6	theme	hydrolytic	372:381	arg1	enzymes					383:389	hydrolytic enzymes	372:389	hydrolytic enzymes	372:389	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	9	7	theme	simulations	1554:1564	arg1	structures					1508:1517	the crystallographic structures	1487:1517	the crystallographic structures of CDs and their molecular dynamic simulations	1487:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	6	8	theme	domain	1040:1045	arg1	structure					1017:1025	the 3D structure	1010:1025	the 3D structure of catalytic domain	1010:1045	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	5	9	theme	available	768:776	arg1	structures					790:799	currently available GH7 crystal structures	758:799	currently available GH7 crystal structures	758:799	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	11	10	theme	catalytic	2000:2008	arg1	cycle					2010:2014	the catalytic cycle	1996:2014	the catalytic cycle of the enzyme	1996:2028	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	10	11	theme	higher	1723:1728	arg1	fluctuations					1745:1756	higher conformational fluctuations	1723:1756	higher conformational fluctuations	1723:1756	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	5	12	theme	crystal	782:788	arg1	structures					790:799	currently available GH7 crystal structures	758:799	currently available GH7 crystal structures	758:799	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	2	13	theme	individual	283:292	arg1	polymers					304:311	individual cellulose polymers	283:311	individual cellulose polymers	283:311	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	12	14	theme	MtCel7A	2089:2095	arg1	DATABASE					2031:2038	DATABASE	2031:2038	DATABASE	2031:2038	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	12	14	theme	MtCel7A	2089:2095	arg1	factors					2078:2084	structural factors	2067:2084	structural factors	2067:2084	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	6	15	theme	3D	1014:1015	arg1	structure					1017:1025	the 3D structure	1010:1025	the 3D structure of catalytic domain	1010:1045	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	11	16	theme	MtCel7A	1953:1959	arg1	site					1945:1948	the catalytic site	1931:1948	the catalytic site of MtCel7A	1931:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	11	16	theme	MtCel7A	1953:1959	arg1	MtCel7A					1953:1959	MtCel7A	1953:1959	MtCel7A	1953:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	12	17	with	Bank	2137:2140	arg1	number					2157:2162	accession number 5W11	2147:2167	accession number 5W11	2147:2167	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	3	18	theme	carbohydrate-binding	629:648	arg1	module					650:655	a CBM1-type carbohydrate-binding module	617:655	a CBM1-type carbohydrate-binding module	617:655	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	2	19	theme	crystalline	321:331	arg1	cellulose					333:341	crystalline cellulose	321:341	crystalline cellulose	321:341	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	9	20	theme	higher	1612:1617	arg1	flexibility					1619:1629	considerably higher flexibility	1599:1629	considerably higher flexibility than TrCel7A	1599:1642	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	6	21	theme	enzyme	1105:1110	arg1	envelope					1112:1119	the full-length enzyme envelope	1089:1119	the full-length enzyme envelope	1089:1119	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	5	22	theme	operation	970:978	arg1	mode					962:965	the catalytic mode	948:965	the catalytic mode of operation	948:978	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	11	23	theme	clusters	1911:1918	arg1	co-evolution					1884:1895	co-evolution	1884:1895	co-evolution	1884:1895	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	5	24	theme	cellulose-binding	847:863	arg1	modules					865:871	cellulose-binding modules	847:871	cellulose-binding modules	847:871	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	3	25	theme	abundant	421:428	arg1	MtCel7A					544:550	the cellobiohydrolase MtCel7A	522:550	the cellobiohydrolase MtCel7A	522:550	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	3	25	theme	abundant	421:428	arg1	enzyme					430:435	The most abundant enzyme	412:435	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass	412:517	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	11	26	theme	amino	1900:1904	arg1	clusters					1911:1918	amino acid clusters	1900:1918	amino acid clusters comprising the catalytic site of MtCel7A	1900:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	6	27	theme	X-ray	1053:1057	arg1	crystallography					1059:1073	X-ray crystallography	1053:1073	X-ray crystallography	1053:1073	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	3	28	theme	biomass	511:517	arg1	presence					499:506	the presence	495:506	the presence of biomass	495:517	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	5	29	theme	separate	813:820	arg1	domains					832:838	separate catalytic domains	813:838	separate catalytic domains	813:838	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	3	30	theme	Myceliophthora thermophila	449:474	arg1	M77					476:478	Myceliophthora thermophila M77	449:478	Myceliophthora thermophila M77	449:478	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	1	31	theme	glucose	169:175	arg1	monomers					177:184	glucose monomers	169:184	glucose monomers linked exclusively by β-1,4 glycosidic linkages	169:232	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	0	32	theme	Biochemical	0:10	arg1	insights					27:34	Biochemical and structural insights	0:34	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila	0:104	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila.					
29222836	1	33	with	polymer	156:162	arg1	monomers					177:184	glucose monomers	169:184	glucose monomers linked exclusively by β-1,4 glycosidic linkages	169:232	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	0	34	theme	structural	16:25	arg1	insights					27:34	Biochemical and structural insights	0:34	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila	0:104	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila.					
29222836	10	35	from	particular	1648:1657	arg1	flexible					1702:1709	flexible	1702:1709	flexible	1702:1709	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	6	36	theme	X-ray	1137:1141	arg1	SAXS					1155:1158	SAXS	1155:1158	SAXS	1155:1158	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	6	36	theme	X-ray	1137:1141	arg1	scattering					1143:1152	small-angle X-ray scattering	1125:1152	small-angle X-ray scattering (SAXS) technique	1125:1169	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	9	37	contain	has	1595:1597	arg1	MtCel7A					1587:1593	MtCel7A	1587:1593	MtCel7A	1587:1593	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	9	37	contain	has	1595:1597	arg2	flexibility					1619:1629	considerably higher flexibility	1599:1629	considerably higher flexibility than TrCel7A	1599:1642	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	7	38	theme	rigid	1231:1235	arg1	linker					1237:1242	a rigid linker	1229:1242	a rigid linker connecting the CD and CBM	1229:1268	The SAXS data reveal a tadpole-like molecular shape with a rigid linker connecting the CD and CBM.					
29222836	0	39	theme	thermostable	43:54	arg1	cellobiohydrolase					56:72	a thermostable cellobiohydrolase	41:72	a thermostable cellobiohydrolase from Myceliophthora thermophila	41:104	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila.					
29222836	11	40	theme	coupling	1857:1864	arg1	analysis					1866:1873	Our statistical coupling analysis	1841:1873	Our statistical coupling analysis	1841:1873	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	10	41	theme	enhanced	1810:1817	arg1	efficiency					1829:1838	enhanced enzymatic efficiency	1810:1838	enhanced enzymatic efficiency	1810:1838	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	4	42	theme	GH7	658:660	arg1	cellobiohydrolases					662:679	GH7 cellobiohydrolases	658:679	GH7 cellobiohydrolases	658:679	GH7 cellobiohydrolases have been studied before, and structural models have been proposed.					
29222836	10	43	theme	enzymatic	1819:1827	arg1	efficiency					1829:1838	enhanced enzymatic efficiency	1810:1838	enhanced enzymatic efficiency	1810:1838	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	9	44	theme	molecular	1536:1544	arg1	simulations					1554:1564	their molecular dynamic simulations	1530:1564	their molecular dynamic simulations	1530:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	7	45	theme	tadpole-like	1195:1206	arg1	shape					1218:1222	a tadpole-like molecular shape	1193:1222	a tadpole-like molecular shape with a rigid linker connecting the CD and CBM	1193:1268	The SAXS data reveal a tadpole-like molecular shape with a rigid linker connecting the CD and CBM.					
29222836	9	46	theme	structures	1508:1517	arg1	comparison					1473:1482	a comparison	1471:1482	a comparison of the crystallographic structures of CDs and their molecular dynamic simulations	1471:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	10	47	from	flexible	1702:1709	arg1	particular					1648:1657	particular	1648:1657	particular	1648:1657	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	10	48	theme	active	1681:1686	arg1	site					1688:1691	the active site	1677:1691	the active site	1677:1691	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	9	49	theme	CDs	1522:1524	arg1	structures					1508:1517	the crystallographic structures	1487:1517	the crystallographic structures of CDs and their molecular dynamic simulations	1487:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	7	50	theme	SAXS	1176:1179	arg1	data					1181:1184	The SAXS data	1172:1184	The SAXS data	1172:1184	The SAXS data reveal a tadpole-like molecular shape with a rigid linker connecting the CD and CBM.					
29222836	9	51	theme	dynamic	1546:1552	arg1	simulations					1554:1564	their molecular dynamic simulations	1530:1564	their molecular dynamic simulations	1530:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
29222836	1	52	theme	β-1,4	208:212	arg1	linkages					225:232	β-1,4 glycosidic linkages	208:232	β-1,4 glycosidic linkages	208:232	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	12	53	theme	Data	2132:2135	arg1	Bank					2137:2140	the Protein Data Bank	2120:2140	the Protein Data Bank with accession number 5W11	2120:2167	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	4	54	theme	structural	711:720	arg1	models					722:727	structural models	711:727	structural models	711:727	GH7 cellobiohydrolases have been studied before, and structural models have been proposed.					
29222836	12	55	dep	DATABASE	2031:2038	arg1	coordinates					2051:2061	The atomic coordinates	2040:2061	The atomic coordinates	2040:2061	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	2	56	theme	enzymes	383:389	arg1	access					362:367	the access	358:367	the access of hydrolytic enzymes and water molecules	358:409	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	5	57	theme	catalytic	952:960	arg1	mode					962:965	the catalytic mode	948:965	the catalytic mode of operation	948:978	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	8	58	contain	has	1313:1315	arg1	MtCel7A					1305:1311	MtCel7A	1305:1311	MtCel7A	1305:1311	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	8	58	contain	has	1313:1315	arg2	thermostability					1349:1363	thermostability	1349:1363	thermostability	1349:1363	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	8	58	contain	has	1313:1315	arg2	processivity					1382:1393	lower processivity	1376:1393	higher catalytic efficiency and thermostability as well as lower processivity	1317:1393	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	8	58	contain	has	1313:1315	arg2	efficiency					1334:1343	higher catalytic efficiency	1317:1343	higher catalytic efficiency	1317:1343	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	2	59	theme	bonding	259:265	arg1	network					267:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	8	60	theme	higher	1317:1322	arg1	efficiency					1334:1343	higher catalytic efficiency	1317:1343	higher catalytic efficiency	1317:1343	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	6	61	theme	catalytic	1030:1038	arg1	domain					1040:1045	catalytic domain	1030:1045	catalytic domain	1030:1045	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	10	62	theme	conformational	1730:1743	arg1	fluctuations					1745:1756	higher conformational fluctuations	1723:1756	higher conformational fluctuations	1723:1756	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	11	63	theme	catalytic	1935:1943	arg1	site					1945:1948	the catalytic site	1931:1948	the catalytic site of MtCel7A	1931:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	11	63	theme	catalytic	1935:1943	arg1	MtCel7A					1953:1959	MtCel7A	1953:1959	MtCel7A	1953:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	5	64	theme	GH7	778:780	arg1	structures					790:799	currently available GH7 crystal structures	758:799	currently available GH7 crystal structures	758:799	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	2	65	theme	cellulose	294:302	arg1	polymers					304:311	individual cellulose polymers	283:311	individual cellulose polymers	283:311	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	8	66	theme	biochemical	1275:1285	arg1	studies					1287:1293	Our biochemical studies	1271:1293	Our biochemical studies	1271:1293	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	0	67	from	Myceliophthora thermophila	79:104	arg1	cellobiohydrolase					56:72	a thermostable cellobiohydrolase	41:72	a thermostable cellobiohydrolase from Myceliophthora thermophila	41:104	Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila.					
29222836	3	68	theme	CBM1-type	619:627	arg1	module					650:655	a CBM1-type carbohydrate-binding module	617:655	a CBM1-type carbohydrate-binding module	617:655	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	1	69	theme	linear	149:154	arg1	cellulose					136:144	cellulose	136:144	cellulose	136:144	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	1	69	theme	linear	149:154	arg1	polymer					156:162	a linear polymer	147:162	a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages	147:232	Cellobiohydrolases hydrolyze cellulose, a linear polymer with glucose monomers linked exclusively by β-1,4 glycosidic linkages.					
29222836	2	70	theme	hydrogen	250:257	arg1	network					267:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	8	71	theme	lower	1376:1380	arg1	processivity					1382:1393	lower processivity	1376:1393	higher catalytic efficiency and thermostability as well as lower processivity	1317:1393	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	2	72	theme	water	395:399	arg1	molecules					401:409	water molecules	395:409	water molecules	395:409	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	5	73	theme	catalytic	822:830	arg1	domains					832:838	separate catalytic domains	813:838	separate catalytic domains	813:838	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	6	74	theme	full-length	1093:1103	arg1	envelope					1112:1119	the full-length enzyme envelope	1089:1119	the full-length enzyme envelope	1089:1119	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	12	75	theme	atomic	2044:2049	arg1	coordinates					2051:2061	The atomic coordinates	2040:2061	The atomic coordinates	2040:2061	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	8	76	theme	well-studied	1416:1427	arg1	TrCel7A					1429:1435	the well-studied TrCel7A	1412:1435	the well-studied TrCel7A from Trichoderma reesei	1412:1459	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	11	77	theme	acid	1906:1909	arg1	clusters					1911:1918	amino acid clusters	1900:1918	amino acid clusters comprising the catalytic site of MtCel7A	1900:1959	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	11	78	from	steps	1987:1991	arg1	cycle					2010:2014	the catalytic cycle	1996:2014	the catalytic cycle of the enzyme	1996:2028	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	11	79	theme	enzyme	2023:2028	arg1	cycle					2010:2014	the catalytic cycle	1996:2014	the catalytic cycle of the enzyme	1996:2028	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	5	80	theme	full-length	896:906	arg1	structures					908:917	the full-length structures	892:917	the full-length structures that are involved in shaping the catalytic mode of operation	892:978	However, currently available GH7 crystal structures only define separate catalytic domains and/or cellulose-binding modules and do not include the full-length structures that are involved in shaping the catalytic mode of operation.					
29222836	6	81	theme	scattering	1143:1152	arg1	technique					1161:1169	small-angle X-ray scattering (SAXS) technique	1125:1169	small-angle X-ray scattering (SAXS) technique	1125:1169	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	10	82	theme	decreased	1783:1791	arg1	processivity					1793:1804	decreased processivity	1783:1804	decreased processivity	1783:1804	In particular, loops that cover the active site are more flexible and undergo higher conformational fluctuations, which might account for decreased processivity and enhanced enzymatic efficiency.					
29222836	3	83	dep	presence	499:506	arg1	response					483:490	response	483:490	response	483:490	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	6	84	theme	small-angle	1125:1135	arg1	SAXS					1155:1158	SAXS	1155:1158	SAXS	1155:1158	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	6	84	theme	small-angle	1125:1135	arg1	scattering					1143:1152	small-angle X-ray scattering	1125:1152	small-angle X-ray scattering (SAXS) technique	1125:1169	In this study, we determined the 3D structure of catalytic domain using X-ray crystallography and retrieved the full-length enzyme envelope via small-angle X-ray scattering (SAXS) technique.					
29222836	12	85	theme	structural	2067:2076	arg1	factors					2078:2084	structural factors	2067:2084	structural factors	2067:2084	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	3	86	theme	cellobiohydrolase	526:542	arg1	MtCel7A					544:550	the cellobiohydrolase MtCel7A	522:550	the cellobiohydrolase MtCel7A	522:550	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	3	86	theme	cellobiohydrolase	526:542	arg1	enzyme					430:435	The most abundant enzyme	412:435	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass	412:517	The most abundant enzyme secreted by Myceliophthora thermophila M77 in response to the presence of biomass is the cellobiohydrolase MtCel7A, which is composed by a GH7-catalytic domain (CD), a linker, and a CBM1-type carbohydrate-binding module.					
29222836	11	87	theme	statistical	1845:1855	arg1	analysis					1866:1873	Our statistical coupling analysis	1841:1873	Our statistical coupling analysis	1841:1873	Our statistical coupling analysis suggests co-evolution of amino acid clusters comprising the catalytic site of MtCel7A, which correlate with the steps in the catalytic cycle of the enzyme.					
29222836	2	88	theme	molecules	401:409	arg1	access					362:367	the access	358:367	the access of hydrolytic enzymes and water molecules	358:409	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	8	89	from	Trichoderma reesei	1442:1459	arg1	TrCel7A					1429:1435	the well-studied TrCel7A	1412:1435	the well-studied TrCel7A from Trichoderma reesei	1412:1459	Our biochemical studies show that MtCel7A has higher catalytic efficiency and thermostability as well as lower processivity when compared to the well-studied TrCel7A from Trichoderma reesei.					
29222836	2	90	theme	widespread	239:248	arg1	network					267:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network	235:273	The widespread hydrogen bonding network tethers individual cellulose polymers forming crystalline cellulose, which prevent the access of hydrolytic enzymes and water molecules.					
29222836	12	91	theme	accession	2147:2155	arg1	number					2157:2162	accession number 5W11	2147:2167	accession number 5W11	2147:2167	DATABASE The atomic coordinates and structural factors of MtCel7A have been deposited in the Protein Data Bank with accession number 5W11.					
29222836	9	92	theme	crystallographic	1491:1506	arg1	structures					1508:1517	the crystallographic structures	1487:1517	the crystallographic structures of CDs and their molecular dynamic simulations	1487:1564	Based on a comparison of the crystallographic structures of CDs and their molecular dynamic simulations, we demonstrate that MtCel7A has considerably higher flexibility than TrCel7A.					
31364612	4	0	theme	N-linked	947:954	arg1	glycans					956:962	18 N-linked glycans	944:962	18 N-linked glycans	944:962	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	2	1	theme	protein	575:581	arg1	interference					583:594	residual protein interference	566:594	residual protein interference	566:594	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	3	2	theme	detection	707:715	arg1	low					730:732	low	730:732	low	730:732	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	3	2	theme	detection	707:715	arg1	limit					717:721	the detection limit	703:721	the detection limit	703:721	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	5	3	theme	S/N	1103:1105	arg1	>					1107:1107	S/N > 10	1103:1110	S/N > 10	1103:1110	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	5	4	theme	N-linked	1081:1088	arg1	glycans					1090:1096	up to 53 N-linked glycans	1072:1096	up to 53 N-linked glycans with S/N > 10	1072:1110	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	5	theme	serum	1419:1423	arg1	samples					1425:1431	the real clinical serum samples	1401:1431	the real clinical serum samples of ovarian cancer patients	1401:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	6	6	theme	real	1405:1408	arg1	samples					1425:1431	the real clinical serum samples	1401:1431	the real clinical serum samples of ovarian cancer patients	1401:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	7	theme	glycans	159:165	arg1	profiling					137:145	the profiling	133:145	the profiling of N-linked glycans	133:165	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	4	8	theme	signal	1004:1009	arg1	intensities					1011:1021	sufficient signal intensities	993:1021	sufficient signal intensities	993:1021	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	6	9	link	N-linked	1379:1386	arg1	glycans					1388:1394	the up-regulated and down-regulated N-linked glycans	1343:1394	the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1343:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	10	theme	size-exclusion	536:549	arg1	effect					551:556	the size-exclusion effect	532:556	not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference	477:594	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	3	11	theme	0.25	737:740	arg1	ng					742:743	ng	742:743	ng	742:743	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	4	12	theme	digest	846:851	arg1	1 					927:928	1 	927:928	1 	927:928	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	4	12	theme	digest	846:851	arg1	ratio					823:827	the mass ratio	814:827	the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin	814:921	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	3	13	theme	protein	612:618	arg1	digest					630:635	a standard protein ovalbumin digest	601:635	a standard protein ovalbumin digest	601:635	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	2	14	from	efficiency	491:500	arg1	enrichment					512:521	glycan enrichment	505:521	glycan enrichment	505:521	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	6	15	theme	cancer	1444:1449	arg1	patients					1451:1458	ovarian cancer patients	1436:1458	ovarian cancer patients	1436:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	5	16	dep	53	1078:1079	arg1	to					1075:1076	to	1075:1076	to	1075:1076	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	0	17	theme	porous	62:67	arg1	composite					81:89	a highly porous 3D graphene composite	53:89	a highly porous 3D graphene composite	53:89	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	1	18	theme	KOH	331:333	arg1	activation					335:344	KOH activation	331:344	KOH activation	331:344	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	6	19	theme	complicated	1538:1548	arg1	samples					1561:1567	minute and complicated biological samples	1527:1567	minute and complicated biological samples	1527:1567	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	20	theme	residual	566:573	arg1	interference					583:594	residual protein interference	566:594	residual protein interference	566:594	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	5	21	link	N-linked	1176:1183	arg1	glycans					1185:1191	only 20 N-linked glycans	1168:1191	only 20 N-linked glycans	1168:1191	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	5	22	theme	graphene	1141:1148	arg1	enrichment					1150:1159	the 3D graphene enrichment	1134:1159	the 3D graphene enrichment	1134:1159	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	3	23	theme	N-linked	780:787	arg1	glycans					789:795	13 N-linked glycans	777:795	13 N-linked glycans (S/N > 10)	777:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	3	23	theme	N-linked	780:787	arg1	>					802:802	S/N > 10	798:805	S/N > 10	798:805	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	0	24	theme	graphene	72:79	arg1	composite					81:89	a highly porous 3D graphene composite	53:89	a highly porous 3D graphene composite	53:89	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	3	25	with	μL-1	745:748	arg1	identification					759:772	the identification	755:772	the identification of 13 N-linked glycans (S/N > 10)	755:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	2	26	theme	high	486:489	arg1	efficiency					491:500	high efficiency	486:500	not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference	477:594	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	1	27	link	N-linked	150:157	arg1	glycans					159:165	N-linked glycans	150:165	N-linked glycans	150:165	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	6	28	from	samples	1425:1431	arg1	glycans					1388:1394	the up-regulated and down-regulated N-linked glycans	1343:1394	the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1343:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	29	theme	mesoporous	416:425	arg1	structure					427:435	3D-layered mesoporous structure	405:435	3D-layered mesoporous structure	405:435	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	5	30	theme	porous	1216:1221	arg1	material					1242:1249	the porous graphitized carbon material	1212:1249	the porous graphitized carbon material used for comparison	1212:1269	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	2	31	theme	surface	370:376	arg1	area					378:381	the large surface area	360:381	the large surface area (ca. 2213 m2 g-1)	360:399	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	31	theme	surface	370:376	arg1	g-1					396:398	ca. 2213 m2 g-1	384:398	ca. 2213 m2 g-1	384:398	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	6	32	theme	3D	1308:1309	arg1	composite					1320:1328	the 3D graphene composite	1304:1328	the 3D graphene composite	1304:1328	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	33	theme	graphene	248:255	arg1	nanosheets					263:272	graphene oxide nanosheets	248:272	graphene oxide nanosheets	248:272	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	6	34	theme	biological	1550:1559	arg1	samples					1561:1567	minute and complicated biological samples	1527:1567	minute and complicated biological samples	1527:1567	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	6	35	theme	graphene	1494:1501	arg1	composite					1503:1511	the 3D graphene composite	1487:1511	the 3D graphene composite	1487:1511	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	5	36	theme	N-linked	1176:1183	arg1	glycans					1185:1191	only 20 N-linked glycans	1168:1191	only 20 N-linked glycans	1168:1191	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	2	37	theme	3D	442:443	arg1	composite					454:462	the 3D graphene composite	438:462	the 3D graphene composite	438:462	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	38	theme	2213	388:391	arg1	area					378:381	the large surface area	360:381	the large surface area (ca. 2213 m2 g-1)	360:399	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	38	theme	2213	388:391	arg1	g-1					396:398	ca. 2213 m2 g-1	384:398	ca. 2213 m2 g-1	384:398	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	0	39	theme	serum	27:31	arg1	glycans					42:48	serum N-linked glycans	27:48	serum N-linked glycans	27:48	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	5	40	theme	complex	1044:1050	arg1	sample					1064:1069	a 60 nL minute complex human serum sample	1029:1069	a 60 nL minute complex human serum sample	1029:1069	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	0	41	theme	glycans	42:48	arg1	profiling					14:22	The efficient profiling	0:22	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite	0:89	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	5	42	theme	serum	1058:1062	arg1	sample					1064:1069	a 60 nL minute complex human serum sample	1029:1069	a 60 nL minute complex human serum sample	1029:1069	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	3	43	link	N-linked	641:648	arg1	glycans					650:656	26 N-linked glycans	638:656	26 N-linked glycans	638:656	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	5	44	with	glycans	1090:1096	arg1	>					1107:1107	S/N > 10	1103:1110	S/N > 10	1103:1110	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	45	theme	down-regulated	1364:1377	arg1	glycans					1388:1394	the up-regulated and down-regulated N-linked glycans	1343:1394	the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1343:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	4	46	theme	sufficient	993:1002	arg1	intensities					1011:1021	sufficient signal intensities	993:1021	sufficient signal intensities	993:1021	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	5	47	theme	carbon	1235:1240	arg1	material					1242:1249	the porous graphitized carbon material	1212:1249	the porous graphitized carbon material used for comparison	1212:1269	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	48	theme	up-regulated	1347:1358	arg1	glycans					1388:1394	the up-regulated and down-regulated N-linked glycans	1343:1394	the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1343:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	49	theme	porous	203:208	arg1	composite					222:230	a highly porous 3D graphene composite	194:230	a highly porous 3D graphene composite fabricated from graphene oxide nanosheets	194:272	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	5	50	theme	human	1052:1056	arg1	sample					1064:1069	a 60 nL minute complex human serum sample	1029:1069	a 60 nL minute complex human serum sample	1029:1069	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	1	51	theme	graphene	213:220	arg1	composite					222:230	a highly porous 3D graphene composite	194:230	a highly porous 3D graphene composite fabricated from graphene oxide nanosheets	194:272	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	3	52	theme	good	679:682	arg1	repeatability					684:696	good repeatability	679:696	good repeatability	679:696	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	4	53	dep	1 	927:928	arg1	 2000					937:941	 2000	937:941	 2000	937:941	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	4	53	dep	1 	927:928	arg1	 2000 					930:935	 2000 	930:935	 2000 	930:935	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	6	54	theme	composite	1320:1328	arg1	application					1289:1299	the application	1285:1299	the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1285:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	4	55	theme	mass	818:821	arg1	1 					927:928	1 	927:928	1 	927:928	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	4	55	theme	mass	818:821	arg1	ratio					823:827	the mass ratio	814:827	the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin	814:921	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	5	56	link	N-linked	1081:1088	arg1	glycans					1090:1096	up to 53 N-linked glycans	1072:1096	up to 53 N-linked glycans with S/N > 10	1072:1110	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	2	57	from	effect	551:556	arg1	enrichment					512:521	glycan enrichment	505:521	glycan enrichment	505:521	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	6	58	theme	clinical	1410:1417	arg1	samples					1425:1431	the real clinical serum samples	1401:1431	the real clinical serum samples of ovarian cancer patients	1401:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	59	theme	N-linked	150:157	arg1	glycans					159:165	N-linked glycans	150:165	N-linked glycans	150:165	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	5	60	theme	nL	1034:1035	arg1	sample					1064:1069	a 60 nL minute complex human serum sample	1029:1069	a 60 nL minute complex human serum sample	1029:1069	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	0	61	link	N-linked	33:40	arg1	glycans					42:48	serum N-linked glycans	27:48	serum N-linked glycans	27:48	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	4	62	link	N-linked	947:954	arg1	glycans					956:962	18 N-linked glycans	944:962	18 N-linked glycans	944:962	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	4	63	theme	interfering	860:870	arg1	proteins					872:879	the interfering proteins	856:879	the interfering proteins	856:879	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	3	64	theme	ng	742:743	arg1	μL-1					745:748	0.25 ng μL-1	737:748	0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10)	737:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	4	65	dep	albumin	901:907	arg1	i.e.					882:885	i.e.	882:885	i.e.	882:885	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	3	66	theme	standard	603:610	arg1	digest					630:635	a standard protein ovalbumin digest	601:635	a standard protein ovalbumin digest	601:635	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	4	67	theme	ovalbumin	836:844	arg1	digest					846:851	the ovalbumin digest	832:851	the ovalbumin digest	832:851	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	6	68	theme	patients	1451:1458	arg1	samples					1425:1431	the real clinical serum samples	1401:1431	the real clinical serum samples of ovarian cancer patients	1401:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	69	theme	enrichment	109:118	arg1	approach					120:127	an enrichment approach	106:127	an enrichment approach for the profiling of N-linked glycans	106:165	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	4	70	theme	serum	895:899	arg1	albumin					901:907	bovine serum albumin	888:907	bovine serum albumin	888:907	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	0	71	theme	3D	69:70	arg1	composite					81:89	a highly porous 3D graphene composite	53:89	a highly porous 3D graphene composite	53:89	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	5	72	theme	60	1031:1032	arg1	nL					1034:1035	nL	1034:1035	nL	1034:1035	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	73	theme	ovarian	1436:1442	arg1	patients					1451:1458	ovarian cancer patients	1436:1458	ovarian cancer patients	1436:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	74	theme	graphene	445:452	arg1	composite					454:462	the 3D graphene composite	438:462	the 3D graphene composite	438:462	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	3	75	link	N-linked	780:787	arg1	glycans					789:795	13 N-linked glycans	777:795	13 N-linked glycans (S/N > 10)	777:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	3	75	link	N-linked	780:787	arg1	>					802:802	S/N > 10	798:805	S/N > 10	798:805	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	5	76	theme	3D	1138:1139	arg1	enrichment					1150:1159	the 3D graphene enrichment	1134:1159	the 3D graphene enrichment	1134:1159	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	3	77	theme	S/N	798:800	arg1	glycans					789:795	13 N-linked glycans	777:795	13 N-linked glycans (S/N > 10)	777:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	3	77	theme	S/N	798:800	arg1	>					802:802	S/N > 10	798:805	S/N > 10	798:805	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	5	78	theme	minute	1037:1042	arg1	sample					1064:1069	a 60 nL minute complex human serum sample	1029:1069	a 60 nL minute complex human serum sample	1029:1069	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	79	theme	minute	1527:1532	arg1	samples					1561:1567	minute and complicated biological samples	1527:1567	minute and complicated biological samples	1527:1567	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	3	80	theme	glycans	789:795	arg1	identification					759:772	the identification	755:772	the identification of 13 N-linked glycans (S/N > 10)	755:806	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	4	81	theme	bovine	888:893	arg1	albumin					901:907	bovine serum albumin	888:907	bovine serum albumin	888:907	When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities.					
31364612	0	82	theme	efficient	4:12	arg1	profiling					14:22	The efficient profiling	0:22	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite	0:89	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	6	83	theme	composite	1503:1511	arg1	potential					1474:1482	the potential	1470:1482	the potential of the 3D graphene composite for analyzing minute and complicated biological samples	1470:1567	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	84	theme	3D-layered	405:414	arg1	structure					427:435	3D-layered mesoporous structure	405:435	3D-layered mesoporous structure	405:435	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	85	theme	large	364:368	arg1	area					378:381	the large surface area	360:381	the large surface area (ca. 2213 m2 g-1)	360:399	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	85	theme	large	364:368	arg1	g-1					396:398	ca. 2213 m2 g-1	384:398	ca. 2213 m2 g-1	384:398	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	1	86	theme	oxide	257:261	arg1	nanosheets					263:272	graphene oxide nanosheets	248:272	graphene oxide nanosheets	248:272	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	6	87	theme	3D	1491:1492	arg1	composite					1503:1511	the 3D graphene composite	1487:1511	the 3D graphene composite	1487:1511	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	2	88	theme	m2	393:394	arg1	area					378:381	the large surface area	360:381	the large surface area (ca. 2213 m2 g-1)	360:399	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	88	theme	m2	393:394	arg1	g-1					396:398	ca. 2213 m2 g-1	384:398	ca. 2213 m2 g-1	384:398	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	0	89	theme	N-linked	33:40	arg1	glycans					42:48	serum N-linked glycans	27:48	serum N-linked glycans	27:48	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.					
31364612	3	90	theme	ovalbumin	620:628	arg1	digest					630:635	a standard protein ovalbumin digest	601:635	a standard protein ovalbumin digest	601:635	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	2	91	theme	ca.	384:386	arg1	area					378:381	the large surface area	360:381	the large surface area (ca. 2213 m2 g-1)	360:399	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	2	91	theme	ca.	384:386	arg1	g-1					396:398	ca. 2213 m2 g-1	384:398	ca. 2213 m2 g-1	384:398	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	1	92	theme	phenol-formaldehyde	280:298	arg1	polymer					300:306	a phenol-formaldehyde polymer	278:306	a phenol-formaldehyde polymer via graphitization and KOH activation	278:344	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
31364612	2	93	theme	glycan	505:510	arg1	enrichment					512:521	glycan enrichment	505:521	glycan enrichment	505:521	In tailoring the large surface area (ca. 2213 m2 g-1) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference.					
31364612	6	94	theme	N-linked	1379:1386	arg1	glycans					1388:1394	the up-regulated and down-regulated N-linked glycans	1343:1394	the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients	1343:1458	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	3	95	theme	N-linked	641:648	arg1	glycans					650:656	26 N-linked glycans	638:656	26 N-linked glycans	638:656	For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng μL-1 with the identification of 13 N-linked glycans (S/N > 10).					
31364612	5	96	theme	graphitized	1223:1233	arg1	material					1242:1249	the porous graphitized carbon material	1212:1249	the porous graphitized carbon material used for comparison	1212:1269	From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison.					
31364612	6	97	theme	graphene	1311:1318	arg1	composite					1320:1328	the 3D graphene composite	1304:1328	the 3D graphene composite	1304:1328	In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.					
31364612	1	98	theme	3D	210:211	arg1	composite					222:230	a highly porous 3D graphene composite	194:230	a highly porous 3D graphene composite fabricated from graphene oxide nanosheets	194:272	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation.					
30168606	12	0	theme	HexNAc4	1845:1851	arg1	NeuAc1					1863:1868	HexNAc4 Hex5 Fuc1 NeuAc1	1845:1868	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2)	1845:1877	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	0	theme	HexNAc4	1845:1851	arg1	4511-2					1871:1876	4511-2	1871:1876	4511-2	1871:1876	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	2	1	gly	glycosylation	318:330	arg1	mechanisms					366:375	dynamic disease mechanisms	350:375	dynamic disease mechanisms	350:375	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	2	1	gly	glycosylation	318:330	arg1	onset					385:389	the onset	381:389	the onset of many pathological conditions	381:421	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	6	2	theme	permethylated	989:1001	arg1	glycans					1003:1009	permethylated glycans	989:1009	permethylated glycans derived from human blood sera on C18-LC-MS/MS	989:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	10	3	theme	potential	1598:1606	arg1	candidates					1629:1638	potential diagnostic biomarker candidates	1598:1638	potential diagnostic biomarker candidates	1598:1638	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	3	4	theme	underlying	578:587	arg1	mechanisms					589:598	underlying mechanisms	578:598	underlying mechanisms	578:598	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	9	5	theme	high	1481:1484	arg1	abundance					1486:1494	a relatively high abundance	1468:1494	a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort)	1468:1572	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	5	6	theme	quantitative	935:946	arg1	profiles					966:973	quantitative isomeric N-glycan profiles	935:973	quantitative isomeric N-glycan profiles	935:973	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	4	7	theme	serum	727:731	arg1	glycome					733:739	the serum glycome	723:739	the serum glycome of patients with RBD	723:760	To test this hypothesis, we assessed the serum glycome of patients with RBD and compared to that of healthy controls.					
30168606	7	8	theme	N-glycans	1160:1168	arg1	structures					1170:1179	Sixteen N-glycans structures	1152:1179	Sixteen N-glycans structures	1152:1179	Sixteen N-glycans structures were found to be significantly altered in the RBD cohort (p < 0.05).					
30168606	6	9	from	N-glycans	1127:1135	arg1	cohort					1144:1149	RBD cohort	1140:1149	RBD cohort	1140:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	1	10	theme	α-synucleinopathy-related	165:189	arg1	stage					153:157	a prodromal stage	141:157	a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases	141:247	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	10	11	theme	biomarker	1619:1627	arg1	candidates					1629:1638	potential diagnostic biomarker candidates	1598:1638	potential diagnostic biomarker candidates	1598:1638	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	1	12	theme	neurodegenerative	194:210	arg1	disease					212:218	neurodegenerative disease	194:218	neurodegenerative disease	194:218	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	10	13	theme	neurodegenerative	1677:1693	arg1	processes					1695:1703	underlying neurodegenerative processes	1666:1703	underlying neurodegenerative processes in patients with idiopathic RBD	1666:1735	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	3	14	theme	disease-associated	648:665	arg1	changes					677:683	disease-associated molecular changes	648:683	disease-associated molecular changes	648:683	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	5	15	theme	high-temperature	874:889	arg1	platform					901:908	high-temperature PGC-LC-MS platform	874:908	high-temperature PGC-LC-MS platform	874:908	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	12	16	theme	RBD	1991:1993	arg1	p < 0.001					2004:2012	p < 0.001	2004:2012	p < 0.001	2004:2012	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	16	theme	RBD	1991:1993	arg1	cohorts					1995:2001	idiopathic RBD cohorts	1980:2001	idiopathic RBD cohorts (p < 0.001)	1980:2013	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	11	17	from	different	1789:1797	arg1	addition					1741:1748	addition	1741:1748	addition	1741:1748	In addition, 7 N-glycan isomers were significantly different between controls and RBD patients (p < 0.05).					
30168606	8	18	theme	NeuAc1	1341:1346	arg1	composition					1269:1279	the composition	1265:1279	the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1	1265:1346	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	6	19	theme	human	1024:1028	arg1	sera					1036:1039	human blood sera	1024:1039	human blood sera on C18-LC-MS/MS	1024:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	20	theme	control	1107:1113	arg1	cohort					1116:1121	healthy (control) cohort	1098:1121	healthy (control) cohort	1098:1121	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	20	theme	control	1107:1113	arg1	N-glycans					1127:1135	56 N-glycans	1124:1135	56 N-glycans in RBD cohort	1124:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	21	attach	derived	1011:1017	arg1	sera					1036:1039	human blood sera	1024:1039	human blood sera on C18-LC-MS/MS	1024:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	21	attach	derived	1011:1017	arg2	glycans					1003:1009	permethylated glycans	989:1009	permethylated glycans derived from human blood sera on C18-LC-MS/MS	989:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	8	22	theme	substantial	1367:1377	arg1	difference					1379:1388	the most substantial difference	1358:1388	the most substantial difference between controls and RBD patients (p < 0.01)	1358:1433	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	0	23	theme	behavior	58:65	arg1	disorder					67:74	behavior disorder	58:74	behavior disorder	58:74	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	13	24	theme	idiopathic	2080:2089	arg1	cohort					2095:2100	the idiopathic RBD cohort	2076:2100	the idiopathic RBD cohort	2076:2100	Levels of both these two isomeric structures were higher in the idiopathic RBD cohort.					
30168606	9	25	theme	idiopathic	1551:1560	arg1	cohort					1566:1571	the idiopathic RBD cohort	1547:1571	the idiopathic RBD cohort	1547:1571	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	6	26	theme	healthy	1098:1104	arg1	cohort					1116:1121	healthy (control) cohort	1098:1121	healthy (control) cohort	1098:1121	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	26	theme	healthy	1098:1104	arg1	N-glycans					1127:1135	56 N-glycans	1124:1135	56 N-glycans in RBD cohort	1124:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	12	27	theme	substantial	1933:1943	arg1	difference					1945:1954	the most substantial difference	1924:1954	the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001)	1924:2013	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	1	28	theme	prodromal	143:151	arg1	stage					153:157	a prodromal stage	141:157	a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases	141:247	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	14	29	theme	Further	2103:2109	arg1	studies					2118:2124	Further larger studies	2103:2124	Further larger studies	2103:2124	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	11	30	theme	RBD	1820:1822	arg1	patients					1824:1831	RBD patients	1820:1831	RBD patients (p < 0.05)	1820:1842	In addition, 7 N-glycan isomers were significantly different between controls and RBD patients (p < 0.05).					
30168606	11	30	theme	RBD	1820:1822	arg1	p < 0.05					1834:1841	p < 0.05	1834:1841	p < 0.05	1834:1841	In addition, 7 N-glycan isomers were significantly different between controls and RBD patients (p < 0.05).					
30168606	2	31	theme	post-translational	283:300	arg1	modification					302:313	post-translational modification	283:313	post-translational modification	283:313	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	1	32	theme	Idiopathic	77:86	arg1	sleep					92:96	Idiopathic REM sleep	77:96	Idiopathic REM sleep behavior disorder (iRBD)	77:121	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	14	33	theme	larger	2111:2116	arg1	studies					2118:2124	Further larger studies	2103:2124	Further larger studies	2103:2124	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	2	34	theme	pathological	399:410	arg1	conditions					412:421	many pathological conditions	394:421	many pathological conditions	394:421	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	0	35	theme	LC-MS/MS	0:7	arg1	glycomics					9:17	LC-MS/MS glycomics	0:17	LC-MS/MS glycomics of idiopathic rapid eye movement	0:50	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	1	36	theme	sleep	92:96	arg1	iRBD					117:120	iRBD	117:120	iRBD	117:120	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	1	36	theme	sleep	92:96	arg1	disorder					107:114	Idiopathic REM sleep behavior disorder	77:114	Idiopathic REM sleep behavior disorder (iRBD)	77:121	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	2	37	theme	Emerging	250:257	arg1	evidence					259:266	Emerging evidence	250:266	Emerging evidence	250:266	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	14	38	theme	pathogenetic	2263:2274	arg1	mechanisms					2276:2285	the pathogenetic mechanisms	2259:2285	the pathogenetic mechanisms of RBD	2259:2292	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	3	39	theme	patients	498:505	arg1	pattern					487:493	the glycosylation pattern	469:493	the glycosylation pattern of patients with RBD	469:514	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	5	40	theme	PGC-LC-MS	891:899	arg1	platform					901:908	high-temperature PGC-LC-MS platform	874:908	high-temperature PGC-LC-MS platform	874:908	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	12	41	theme	Fuc1	1896:1899	arg1	NeuAc2					1901:1906	HexNAc4 Hex5 Fuc1 NeuAc2	1883:1906	HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2)	1883:1915	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	41	theme	Fuc1	1896:1899	arg1	4512-2					1909:1914	4512-2	1909:1914	4512-2	1909:1914	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	3	42	theme	pattern	487:493	arg1	characterization					449:464	the characterization	445:464	the characterization of the glycosylation pattern of patients with RBD	445:514	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	10	43	theme	idiopathic	1722:1731	arg1	RBD					1733:1735	idiopathic RBD	1722:1735	idiopathic RBD	1722:1735	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	7	44	theme	RBD	1227:1229	arg1	p < 0.05					1239:1246	p < 0.05	1239:1246	p < 0.05	1239:1246	Sixteen N-glycans structures were found to be significantly altered in the RBD cohort (p < 0.05).					
30168606	7	44	theme	RBD	1227:1229	arg1	cohort					1231:1236	the RBD cohort	1223:1236	the RBD cohort (p < 0.05)	1223:1247	Sixteen N-glycans structures were found to be significantly altered in the RBD cohort (p < 0.05).					
30168606	0	45	theme	rapid	33:37	arg1	movement					43:50	idiopathic rapid eye movement	22:50	idiopathic rapid eye movement	22:50	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	10	46	from	processes	1695:1703	arg1	patients					1708:1715	patients	1708:1715	patients with idiopathic RBD	1708:1735	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	9	47	dep	abundance	1486:1494	arg1	%					1542:1542	4 ± 3%	1537:1542	4 ± 3% in the idiopathic RBD cohort	1537:1571	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	9	47	dep	abundance	1486:1494	arg1	%					1506:1506	3.1 ± 0.7%	1497:1506	3.1 ± 0.7% in the control cohort	1497:1528	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	5	48	theme	N-glycan	850:857	arg1	profiles					859:866	quantitative N-glycan profiles	837:866	quantitative N-glycan profiles	837:866	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	15	49	theme	therapeutic	2357:2367	arg1	targets					2369:2375	molecular therapeutic targets	2347:2375	molecular therapeutic targets	2347:2375	This information will be instrumental in developing molecular therapeutic targets to promote neuroprotection and prevention of neurodegeneration.					
30168606	3	50	gly	glycosylation	473:485	arg1	patients					498:505	patients	498:505	patients with RBD	498:514	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	13	51	theme	structures	2050:2059	arg1	Levels					2016:2021	Levels	2016:2021	Levels of both these two isomeric structures	2016:2059	Levels of both these two isomeric structures were higher in the idiopathic RBD cohort.					
30168606	2	52	theme	dynamic	350:356	arg1	mechanisms					366:375	dynamic disease mechanisms	350:375	dynamic disease mechanisms	350:375	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	9	53	theme	HexNAc4	1436:1442	arg1	NeuAc1					1454:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	12	54	theme	HexNAc4	1883:1889	arg1	NeuAc2					1901:1906	HexNAc4 Hex5 Fuc1 NeuAc2	1883:1906	HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2)	1883:1915	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	54	theme	HexNAc4	1883:1889	arg1	4512-2					1909:1914	4512-2	1909:1914	4512-2	1909:1914	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	9	55	theme	Fuc1	1449:1452	arg1	NeuAc1					1454:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	12	56	theme	Hex5	1853:1856	arg1	NeuAc1					1863:1868	HexNAc4 Hex5 Fuc1 NeuAc1	1845:1868	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2)	1845:1877	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	56	theme	Hex5	1853:1856	arg1	4511-2					1871:1876	4511-2	1871:1876	4511-2	1871:1876	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	3	57	theme	great	528:532	arg1	value					534:538	great value	528:538	great value	528:538	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	10	58	with	patients	1708:1715	arg1	RBD					1733:1735	idiopathic RBD	1722:1735	idiopathic RBD	1722:1735	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	10	59	theme	diagnostic	1608:1617	arg1	candidates					1629:1638	potential diagnostic biomarker candidates	1598:1638	potential diagnostic biomarker candidates	1598:1638	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	4	60	with	patients	744:751	arg1	RBD					758:760	RBD	758:760	RBD	758:760	To test this hypothesis, we assessed the serum glycome of patients with RBD and compared to that of healthy controls.					
30168606	8	61	theme	Hex5	1312:1315	arg1	composition					1269:1279	the composition	1265:1279	the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1	1265:1346	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	0	62	theme	movement	43:50	arg1	glycomics					9:17	LC-MS/MS glycomics	0:17	LC-MS/MS glycomics of idiopathic rapid eye movement	0:50	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	9	63	from	%	1542:1542	arg1	cohort					1566:1571	the idiopathic RBD cohort	1547:1571	the idiopathic RBD cohort	1547:1571	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	8	64	theme	Fuc1	1297:1300	arg1	composition					1269:1279	the composition	1265:1279	the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1	1265:1346	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	5	65	theme	isomeric	948:955	arg1	profiles					966:973	quantitative isomeric N-glycan profiles	935:973	quantitative isomeric N-glycan profiles	935:973	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	10	66	theme	underlying	1666:1675	arg1	processes					1695:1703	underlying neurodegenerative processes	1666:1703	underlying neurodegenerative processes in patients with idiopathic RBD	1666:1735	These N-glycans can be potential diagnostic biomarker candidates and provide a window into underlying neurodegenerative processes in patients with idiopathic RBD.					
30168606	3	67	with	patients	498:505	arg1	RBD					512:514	RBD	512:514	RBD	512:514	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	15	68	theme	neurodegeneration	2422:2438	arg1	prevention					2408:2417	prevention	2408:2417	prevention	2408:2417	This information will be instrumental in developing molecular therapeutic targets to promote neuroprotection and prevention of neurodegeneration.					
30168606	15	68	theme	neurodegeneration	2422:2438	arg1	neuroprotection					2388:2402	neuroprotection	2388:2402	neuroprotection	2388:2402	This information will be instrumental in developing molecular therapeutic targets to promote neuroprotection and prevention of neurodegeneration.					
30168606	5	69	theme	N-glycan	957:964	arg1	profiles					966:973	quantitative isomeric N-glycan profiles	935:973	quantitative isomeric N-glycan profiles	935:973	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	9	70	theme	control	1515:1521	arg1	cohort					1523:1528	the control cohort	1511:1528	the control cohort	1511:1528	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	3	71	theme	useful	626:631	arg1	biomarkers					633:642	potentially useful biomarkers	614:642	potentially useful biomarkers for disease-associated molecular changes	614:683	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	12	72	theme	idiopathic	1980:1989	arg1	p < 0.001					2004:2012	p < 0.001	2004:2012	p < 0.001	2004:2012	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	72	theme	idiopathic	1980:1989	arg1	cohorts					1995:2001	idiopathic RBD cohorts	1980:2001	idiopathic RBD cohorts (p < 0.001)	1980:2013	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	6	73	theme	blood	1030:1034	arg1	sera					1036:1039	human blood sera	1024:1039	human blood sera on C18-LC-MS/MS	1024:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	8	74	with	N-glycans	1250:1258	arg1	composition					1269:1279	the composition	1265:1279	the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1	1265:1346	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	3	75	theme	molecular	667:675	arg1	changes					677:683	disease-associated molecular changes	648:683	disease-associated molecular changes	648:683	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	14	76	from	structures	2245:2254	arg1	mechanisms					2276:2285	the pathogenetic mechanisms	2259:2285	the pathogenetic mechanisms of RBD	2259:2292	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	15	77	theme	molecular	2347:2355	arg1	targets					2369:2375	molecular therapeutic targets	2347:2375	molecular therapeutic targets	2347:2375	This information will be instrumental in developing molecular therapeutic targets to promote neuroprotection and prevention of neurodegeneration.					
30168606	13	78	theme	RBD	2091:2093	arg1	cohort					2095:2100	the idiopathic RBD cohort	2076:2100	the idiopathic RBD cohort	2076:2100	Levels of both these two isomeric structures were higher in the idiopathic RBD cohort.					
30168606	9	79	theme	RBD	1562:1564	arg1	cohort					1566:1571	the idiopathic RBD cohort	1547:1571	the idiopathic RBD cohort	1547:1571	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	4	80	theme	patients	744:751	arg1	glycome					733:739	the serum glycome	723:739	the serum glycome of patients with RBD	723:760	To test this hypothesis, we assessed the serum glycome of patients with RBD and compared to that of healthy controls.					
30168606	6	81	theme	N-glycan	1075:1082	arg1	structures					1084:1093	59 N-glycan structures	1072:1093	59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort	1072:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	11	82	from	addition	1741:1748	arg1	different					1789:1797	different	1789:1797	different	1789:1797	In addition, 7 N-glycan isomers were significantly different between controls and RBD patients (p < 0.05).					
30168606	5	83	used	used	820:823	arg2	NanoRPLC-MS					804:814	NanoRPLC-MS	804:814	NanoRPLC-MS	804:814	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	14	84	theme	findings	2178:2185	arg1	reproducibility					2153:2167	the reproducibility	2149:2167	the reproducibility of these findings	2149:2185	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	8	85	theme	RBD	1411:1413	arg1	p < 0.01					1425:1432	p < 0.01	1425:1432	p < 0.01	1425:1432	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	8	85	theme	RBD	1411:1413	arg1	patients					1415:1422	RBD patients	1411:1422	RBD patients (p < 0.01)	1411:1433	N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and HexNAc4 Hex5 Fuc1 NeuAc1 presented the most substantial difference between controls and RBD patients (p < 0.01).					
30168606	14	86	theme	RBD	2290:2292	arg1	mechanisms					2276:2285	the pathogenetic mechanisms	2259:2285	the pathogenetic mechanisms of RBD	2259:2292	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	2	87	theme	conditions	412:421	arg1	mechanisms					366:375	dynamic disease mechanisms	350:375	dynamic disease mechanisms	350:375	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	2	87	theme	conditions	412:421	arg1	onset					385:389	the onset	381:389	the onset of many pathological conditions	381:421	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	1	88	theme	REM	88:90	arg1	sleep					92:96	Idiopathic REM sleep	77:96	Idiopathic REM sleep behavior disorder (iRBD)	77:121	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	6	89	theme	RBD	1140:1142	arg1	cohort					1144:1149	RBD cohort	1140:1149	RBD cohort	1140:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	90	from	structures	1084:1093	arg1	cohort					1116:1121	healthy (control) cohort	1098:1121	healthy (control) cohort	1098:1121	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	6	90	from	structures	1084:1093	arg1	N-glycans					1127:1135	56 N-glycans	1124:1135	56 N-glycans in RBD cohort	1124:1149	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	2	91	theme	many	394:397	arg1	conditions					412:421	many pathological conditions	394:421	many pathological conditions	394:421	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	0	92	theme	idiopathic	22:31	arg1	movement					43:50	idiopathic rapid eye movement	22:50	idiopathic rapid eye movement	22:50	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	1	93	theme	behavior	98:105	arg1	iRBD					117:120	iRBD	117:120	iRBD	117:120	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	1	93	theme	behavior	98:105	arg1	disorder					107:114	Idiopathic REM sleep behavior disorder	77:114	Idiopathic REM sleep behavior disorder (iRBD)	77:121	Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases.					
30168606	4	94	theme	healthy	786:792	arg1	controls					794:801	healthy controls	786:801	healthy controls	786:801	To test this hypothesis, we assessed the serum glycome of patients with RBD and compared to that of healthy controls.					
30168606	0	95	theme	eye	39:41	arg1	movement					43:50	idiopathic rapid eye movement	22:50	idiopathic rapid eye movement	22:50	LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.					
30168606	14	96	from	changes	2227:2233	arg1	structures					2245:2254	glycan structures	2238:2254	glycan structures in the pathogenetic mechanisms of RBD	2238:2292	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
30168606	5	97	theme	quantitative	837:848	arg1	profiles					859:866	quantitative N-glycan profiles	837:866	quantitative N-glycan profiles	837:866	NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles.					
30168606	12	98	theme	Fuc1	1858:1861	arg1	NeuAc1					1863:1868	HexNAc4 Hex5 Fuc1 NeuAc1	1845:1868	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2)	1845:1877	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	98	theme	Fuc1	1858:1861	arg1	4511-2					1871:1876	4511-2	1871:1876	4511-2	1871:1876	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	13	99	theme	isomeric	2041:2048	arg1	structures					2050:2059	both these two isomeric structures	2026:2059	both these two isomeric structures	2026:2059	Levels of both these two isomeric structures were higher in the idiopathic RBD cohort.					
30168606	2	100	theme	disease	358:364	arg1	mechanisms					366:375	dynamic disease mechanisms	350:375	dynamic disease mechanisms	350:375	Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions.					
30168606	12	101	theme	Hex5	1891:1894	arg1	NeuAc2					1901:1906	HexNAc4 Hex5 Fuc1 NeuAc2	1883:1906	HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2)	1883:1915	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	12	101	theme	Hex5	1891:1894	arg1	4512-2					1909:1914	4512-2	1909:1914	4512-2	1909:1914	HexNAc4 Hex5 Fuc1 NeuAc1 (4511-2) and HexNAc4 Hex5 Fuc1 NeuAc2 (4512-2) showed the most substantial difference between the control and idiopathic RBD cohorts (p < 0.001).					
30168606	6	102	from	sera	1036:1039	arg1	C18-LC-MS/MS					1044:1055	C18-LC-MS/MS	1044:1055	C18-LC-MS/MS	1044:1055	By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort.					
30168606	11	103	theme	N-glycan	1753:1760	arg1	isomers					1762:1768	7 N-glycan isomers	1751:1768	7 N-glycan isomers	1751:1768	In addition, 7 N-glycan isomers were significantly different between controls and RBD patients (p < 0.05).					
30168606	9	104	theme	Hex5	1444:1447	arg1	NeuAc1					1454:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1	1436:1459	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	9	105	from	%	1506:1506	arg1	cohort					1523:1528	the control cohort	1511:1528	the control cohort	1511:1528	HexNAc4 Hex5 Fuc1 NeuAc1 showed a relatively high abundance (3.1 ± 0.7% in the control cohort versus 4 ± 3% in the idiopathic RBD cohort).					
30168606	3	106	theme	glycosylation	473:485	arg1	pattern					487:493	the glycosylation pattern	469:493	the glycosylation pattern of patients with RBD	469:514	We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes.					
30168606	14	107	theme	glycan	2238:2243	arg1	structures					2245:2254	glycan structures	2238:2254	glycan structures in the pathogenetic mechanisms of RBD	2238:2292	Further larger studies are required to assess the reproducibility of these findings and to elucidate the role played by the changes in glycan structures in the pathogenetic mechanisms of RBD.					
29500371	1	0	from	impact	176:181	arg1	properties					199:208	multifaceted properties	186:208	multifaceted properties of antibodies such as in their effector function, structure, and stability	186:283	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	2	1	theme	mediated	489:496	arg1	ADCC					512:515	ADCC	512:515	ADCC	512:515	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	1	theme	mediated	489:496	arg1	cytotoxicity					498:509	the antibody-dependent cell mediated cytotoxicity	461:509	the antibody-dependent cell mediated cytotoxicity (ADCC)	461:516	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	6	2	theme	major	1090:1094	arg1	galactosylation					1068:1082	Fc galactosylation	1065:1082	Fc galactosylation	1065:1082	Notably, Fc galactosylation was a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column.					
29500371	6	2	theme	major	1090:1094	arg1	factor					1096:1101	a major factor	1088:1101	a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column	1088:1178	Notably, Fc galactosylation was a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column.					
29500371	0	3	theme	FcγReceptor	91:101	arg1	Column					120:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	5	4	theme	N-glycan	998:1005	arg1	composition					979:989	the composition	975:989	the composition of the N-glycan	975:1005	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	4	5	theme	quality	836:842	arg1	control					844:850	quality control	836:850	quality control	836:850	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	2	6	theme	Numerous	286:293	arg1	studies					295:301	Numerous studies	286:301	Numerous studies	286:301	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	0	7	theme	engineered	80:89	arg1	Column					120:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	7	8	theme	antibody	1186:1193	arg1	fraction					1195:1202	Each antibody fraction	1181:1202	Each antibody fraction	1181:1202	Each antibody fraction was employed for structural, biological, and physicochemical analysis, illustrating the mechanism by which galactose modulates the affinity to FcγRIIIa.					
29500371	1	9	contain	has	158:160	arg1	moiety					141:146	The N-glycan moiety	128:146	The N-glycan moiety of IgG-Fc	128:156	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	1	9	contain	has	158:160	arg2	impact					176:181	a significant impact	162:181	a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability	162:283	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	1	10	theme	significant	164:174	arg1	impact					176:181	a significant impact	162:181	a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability	162:283	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	0	11	theme	IIIa-Immobilized	103:118	arg1	Column					120:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	an engineered FcγReceptor IIIa-Immobilized Column	77:125	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	0	12	theme	Fc-Glycan	35:43	arg1	Heterogeneity					14:26	the Heterogeneity	10:26	the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody	10:69	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	4	13	theme	molecular	777:785	arg1	design					787:792	the molecular design	773:792	the molecular design	773:792	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	7	14	theme	structural	1221:1230	arg1	analysis					1265:1272	structural, biological, and physicochemical analysis	1221:1272	structural, biological, and physicochemical analysis	1221:1272	Each antibody fraction was employed for structural, biological, and physicochemical analysis, illustrating the mechanism by which galactose modulates the affinity to FcγRIIIa.					
29500371	4	15	theme	antibodies	867:876	arg1	control					844:850	quality control	836:850	quality control	836:850	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	4	15	theme	antibodies	867:876	arg1	manufacturing					795:807	manufacturing	795:807	manufacturing	795:807	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	4	15	theme	antibodies	867:876	arg1	optimization					818:829	process optimization	810:829	process optimization	810:829	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	4	15	theme	antibodies	867:876	arg1	design					787:792	the molecular design	773:792	the molecular design	773:792	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	2	16	theme	functions	443:451	arg1	magnitude					421:429	the magnitude	417:429	the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC)	417:565	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	4	17	theme	therapeutic	855:865	arg1	antibodies					867:876	therapeutic antibodies	855:876	therapeutic antibodies	855:876	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	8	18	theme	column	1425:1430	arg1	benefits					1385:1392	the benefits	1381:1392	the benefits of the FcγRIIIa chromatography column	1381:1430	In addition, we discuss the benefits of the FcγRIIIa chromatography column to assess the heterogeneity of the N-glycan.					
29500371	1	19	theme	multifaceted	186:197	arg1	properties					199:208	multifaceted properties	186:208	multifaceted properties of antibodies such as in their effector function, structure, and stability	186:283	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	2	20	theme	effector	434:441	arg1	functions					443:451	effector functions	434:451	effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC)	434:565	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	20	theme	effector	434:441	arg1	cytotoxicity					548:559	the complement-dependent cytotoxicity	523:559	the complement-dependent cytotoxicity (CDC)	523:565	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	20	theme	effector	434:441	arg1	cytotoxicity					498:509	the antibody-dependent cell mediated cytotoxicity	461:509	the antibody-dependent cell mediated cytotoxicity (ADCC)	461:516	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	5	21	theme	chromatography	1033:1046	arg1	column					1048:1053	a novel FcγRIIIa chromatography column	1016:1053	a novel FcγRIIIa chromatography column	1016:1053	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	3	22	theme	variants	639:646	arg1	properties					604:613	properties	604:613	properties	604:613	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	3	22	theme	variants	639:646	arg1	influence					619:627	influence	619:627	influence	619:627	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	5	23	theme	FcγRIIIa	1024:1031	arg1	column					1048:1053	a novel FcγRIIIa chromatography column	1016:1053	a novel FcγRIIIa chromatography column	1016:1053	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	3	24	dep	properties	604:613	arg1	the					600:602	the	600:602	the	600:602	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	2	25	theme	cell	484:487	arg1	ADCC					512:515	ADCC	512:515	ADCC	512:515	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	25	theme	cell	484:487	arg1	cytotoxicity					498:509	the antibody-dependent cell mediated cytotoxicity	461:509	the antibody-dependent cell mediated cytotoxicity (ADCC)	461:516	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	6	26	theme	IgG-Fc	1131:1136	arg1	affinity					1119:1126	the affinity	1115:1126	the affinity of IgG-Fc to the FcγRIIIa immobilized on the column	1115:1178	Notably, Fc galactosylation was a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column.					
29500371	7	27	theme	physicochemical	1249:1263	arg1	analysis					1265:1272	structural, biological, and physicochemical analysis	1221:1272	structural, biological, and physicochemical analysis	1221:1272	Each antibody fraction was employed for structural, biological, and physicochemical analysis, illustrating the mechanism by which galactose modulates the affinity to FcγRIIIa.					
29500371	6	28	theme	Fc	1065:1066	arg1	galactosylation					1068:1082	Fc galactosylation	1065:1082	Fc galactosylation	1065:1082	Notably, Fc galactosylation was a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column.					
29500371	6	28	theme	Fc	1065:1066	arg1	factor					1096:1101	a major factor	1088:1101	a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column	1088:1178	Notably, Fc galactosylation was a major factor influencing the affinity of IgG-Fc to the FcγRIIIa immobilized on the column.					
29500371	1	29	theme	antibodies	213:222	arg1	properties					199:208	multifaceted properties	186:208	multifaceted properties of antibodies such as in their effector function, structure, and stability	186:283	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	2	30	theme	Fc	395:396	arg1	N-glycan					398:405	the Fc N-glycan	391:405	the Fc N-glycan	391:405	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	31	theme	antibody-dependent	465:482	arg1	ADCC					512:515	ADCC	512:515	ADCC	512:515	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	31	theme	antibody-dependent	465:482	arg1	cytotoxicity					498:509	the antibody-dependent cell mediated cytotoxicity	461:509	the antibody-dependent cell mediated cytotoxicity (ADCC)	461:516	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	8	32	theme	N-glycan	1467:1474	arg1	heterogeneity					1446:1458	the heterogeneity	1442:1458	the heterogeneity of the N-glycan	1442:1474	In addition, we discuss the benefits of the FcγRIIIa chromatography column to assess the heterogeneity of the N-glycan.					
29500371	5	33	theme	novel	1018:1022	arg1	column					1048:1053	a novel FcγRIIIa chromatography column	1016:1053	a novel FcγRIIIa chromatography column	1016:1053	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	2	34	theme	N-glycan	398:405	arg1	composition					376:386	the exact composition	366:386	the exact composition of the Fc N-glycan	366:405	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	3	35	theme	glycan	632:637	arg1	variants					639:646	glycan variants	632:646	glycan variants	632:646	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	0	36	gly	Heterogeneity	14:26	arg1	Fc-Glycan					35:43	the Fc-Glycan	31:43	the Fc-Glycan of a Therapeutic Antibody	31:69	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	2	37	theme	biological	342:351	arg1	effect					353:358	its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC)	338:565	its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC)	338:565	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	38	theme	complement-dependent	527:546	arg1	CDC					562:564	CDC	562:564	CDC	562:564	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	2	38	theme	complement-dependent	527:546	arg1	cytotoxicity					548:559	the complement-dependent cytotoxicity	523:559	the complement-dependent cytotoxicity (CDC)	523:565	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	8	39	theme	chromatography	1410:1423	arg1	column					1425:1430	the FcγRIIIa chromatography column	1397:1430	the FcγRIIIa chromatography column	1397:1430	In addition, we discuss the benefits of the FcγRIIIa chromatography column to assess the heterogeneity of the N-glycan.					
29500371	3	40	theme	great	661:665	arg1	interest					667:674	great interest	661:674	great interest	661:674	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	3	41	theme	systematic	577:586	arg1	analyses					588:595	systematic analyses	577:595	systematic analyses of the properties and influence of glycan variants	577:646	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	5	42	theme	model	914:918	arg1	antibody					932:939	a model therapeutic antibody	912:939	a model therapeutic antibody	912:939	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	1	43	theme	N-glycan	132:139	arg1	moiety					141:146	The N-glycan moiety	128:146	The N-glycan moiety of IgG-Fc	128:156	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	8	44	gly	heterogeneity	1446:1458	arg1	N-glycan					1467:1474	the N-glycan	1463:1474	the N-glycan	1463:1474	In addition, we discuss the benefits of the FcγRIIIa chromatography column to assess the heterogeneity of the N-glycan.					
29500371	3	45	theme	influence	619:627	arg1	analyses					588:595	systematic analyses	577:595	systematic analyses of the properties and influence of glycan variants	577:646	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	5	46	theme	therapeutic	920:930	arg1	antibody					932:939	a model therapeutic antibody	912:939	a model therapeutic antibody	912:939	In this study, we have separated a model therapeutic antibody into three fractions according to the composition of the N-glycan by using a novel FcγRIIIa chromatography column.					
29500371	0	47	theme	Antibody	62:69	arg1	Fc-Glycan					35:43	the Fc-Glycan	31:43	the Fc-Glycan of a Therapeutic Antibody	31:69	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	4	48	theme	process	810:816	arg1	optimization					818:829	process optimization	810:829	process optimization	810:829	Understanding the principles on how N-glycosylation modulates those properties is important for the molecular design, manufacturing, process optimization, and quality control of therapeutic antibodies.					
29500371	2	49	theme	exact	370:374	arg1	composition					376:386	the exact composition	366:386	the exact composition of the Fc N-glycan	366:405	Numerous studies have been devoted to understanding its biological effect since the exact composition of the Fc N-glycan modulates the magnitude of effector functions such as the antibody-dependent cell mediated cytotoxicity (ADCC), and the complement-dependent cytotoxicity (CDC).					
29500371	7	50	theme	biological	1233:1242	arg1	analysis					1265:1272	structural, biological, and physicochemical analysis	1221:1272	structural, biological, and physicochemical analysis	1221:1272	Each antibody fraction was employed for structural, biological, and physicochemical analysis, illustrating the mechanism by which galactose modulates the affinity to FcγRIIIa.					
29500371	0	51	theme	Therapeutic	50:60	arg1	Antibody					62:69	a Therapeutic Antibody	48:69	a Therapeutic Antibody	48:69	Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.					
29500371	3	52	theme	properties	604:613	arg1	analyses					588:595	systematic analyses	577:595	systematic analyses of the properties and influence of glycan variants	577:646	To date, systematic analyses of the properties and influence of glycan variants have been of great interest.					
29500371	1	53	theme	IgG-Fc	151:156	arg1	moiety					141:146	The N-glycan moiety	128:146	The N-glycan moiety of IgG-Fc	128:156	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
29500371	8	54	theme	FcγRIIIa	1401:1408	arg1	column					1425:1430	the FcγRIIIa chromatography column	1397:1430	the FcγRIIIa chromatography column	1397:1430	In addition, we discuss the benefits of the FcγRIIIa chromatography column to assess the heterogeneity of the N-glycan.					
29500371	1	55	theme	effector	241:248	arg1	function					250:257	their effector function	235:257	their effector function	235:257	The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies such as in their effector function, structure, and stability.					
31817903	13	0	theme	acid	1939:1942	arg1	derivative					1944:1953	an N-acetylmuramic acid derivative	1920:1953	an N-acetylmuramic acid derivative	1920:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	5	1	theme	transglycosylation	704:721	arg1	acceptors					723:731	its transglycosylation acceptors	700:731	its transglycosylation acceptors	700:731	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	2	2	theme	tolerance	384:392	arg1	terms					317:321	terms	317:321	terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications	317:419	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	10	3	theme	β	1548:1548	arg1	position					1555:1562	the β(1-4) position	1544:1562	the β(1-4) position	1544:1562	Moreover, whereas the gluco-configuration at C-4 steered the glycosylation into the β(1-4) position, the galacto-acceptor afforded a β(1-6) glycosidic linkage.					
31817903	13	4	theme	glycosylation	1903:1915	arg1	example					1882:1888	the first example	1872:1888	the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative	1872:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	13	4	theme	glycosylation	1903:1915	arg1	this					1864:1867	this	1864:1867	this	1864:1867	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	5	5	theme	model	665:669	arg1	β-N-acetylhexosaminidase					671:694	this model β-N-acetylhexosaminidase	660:694	this model β-N-acetylhexosaminidase	660:694	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	11	6	theme	uronic	1699:1704	arg1	acid					1706:1709	uronic acid	1699:1709	uronic acid	1699:1709	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	14	7	theme	modeling	2022:2029	arg1	study					2031:2035	a modeling study	2020:2035	a modeling study	2020:2035	In order to explain these findings and predict enzyme behavior, a modeling study was accomplished that correlated with the acquired experimental data.					
31817903	13	8	theme	first	1876:1880	arg1	example					1882:1888	the first example	1872:1888	the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative	1872:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	13	8	theme	first	1876:1880	arg1	this					1864:1867	this	1864:1867	this	1864:1867	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	2	9	theme	synthetic	330:338	arg1	potential					340:348	its synthetic potential	326:348	its synthetic potential	326:348	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	14	10	theme	enzyme	2003:2008	arg1	behavior					2010:2017	enzyme behavior	2003:2017	enzyme behavior	2003:2017	In order to explain these findings and predict enzyme behavior, a modeling study was accomplished that correlated with the acquired experimental data.					
31817903	12	11	theme	latter	1746:1751	arg1	acceptor					1753:1760	the latter acceptor	1742:1760	the latter acceptor	1742:1760	With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield.					
31817903	2	12	from	terms	317:321	arg1	β-N-acetylhexosaminidase					251:274	The β-N-acetylhexosaminidase	247:274	The β-N-acetylhexosaminidase from Talaromyces flavus	247:298	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	2	12	from	terms	317:321	arg1	remarkable					303:312	remarkable	303:312	remarkable	303:312	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	9	13	theme	transglycosylation	1385:1402	arg1	course					1375:1380	the normal course	1364:1380	the normal course of transglycosylation	1364:1402	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	1	14	theme	interest	237:244	arg1	oligosaccharides					206:221	oligosaccharides	206:221	oligosaccharides of biological interest	206:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	12	15	theme	low	1835:1837	arg1	yield					1839:1843	a low yield	1833:1843	a low yield	1833:1843	With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield.					
31817903	11	16	theme	Y470H	1628:1632	arg1	enzyme					1641:1646	The Y470H mutant enzyme	1624:1646	The Y470H mutant enzyme	1624:1646	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	9	17	theme	non-reducing	1421:1432	arg1	disaccharides					1434:1446	unique non-reducing disaccharides	1414:1446	unique non-reducing disaccharides	1414:1446	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	7	18	theme	transglycosylation	1124:1141	arg1	products					1143:1150	The transglycosylation products	1120:1150	The transglycosylation products	1120:1150	The transglycosylation products were isolated and structurally characterized.					
31817903	12	19	theme	glycosylation	1808:1820	arg1	product					1822:1828	one glycosylation product	1804:1828	one glycosylation product	1804:1828	With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield.					
31817903	9	20	theme	low	1453:1455	arg1	yield					1457:1461	a low yield	1451:1461	a low yield	1451:1461	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	9	21	theme	hydroxyl	1330:1337	arg1	moiety					1339:1344	the C-2 hydroxyl moiety	1322:1344	the C-2 hydroxyl moiety	1322:1344	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	1	22	dep	in	158:159	arg1	vivo					161:164	vivo	161:164	vivo	161:164	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	10	23	theme	glycosidic	1604:1613	arg1	linkage					1615:1621	a β(1-6) glycosidic linkage	1595:1621	a β(1-6) glycosidic linkage	1595:1621	Moreover, whereas the gluco-configuration at C-4 steered the glycosylation into the β(1-4) position, the galacto-acceptor afforded a β(1-6) glycosidic linkage.					
31817903	6	24	theme	Enzymatic	788:796	arg1	reactions					798:806	Enzymatic reactions	788:806	Enzymatic reactions	788:806	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	8	25	theme	N-acetamido	1206:1216	arg1	group					1218:1222	The C-2 N-acetamido group	1198:1222	The C-2 N-acetamido group in the acceptor molecule	1198:1247	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	8	25	theme	N-acetamido	1206:1216	arg1	essential					1265:1273	essential	1265:1273	essential	1265:1273	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	5	26	theme	work	608:611	arg1	aim					596:598	The aim	592:598	The aim of this work	592:611	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	4	27	theme	Tyr470	512:517	arg1	residue					519:525	the Tyr470 residue	508:525	the Tyr470 residue	508:525	The mutation of the Tyr470 residue to histidine greatly enhances its transglycosylation capability.					
31817903	0	28	theme	Acceptor	0:7	arg1	Specificity					9:19	Acceptor Specificity	0:19	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus	0:71	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	0	29	dep	Explanation	85:95	arg1	Specificity					9:19	Acceptor Specificity	0:19	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus	0:71	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	8	30	theme	acceptor	1231:1238	arg1	molecule					1240:1247	the acceptor molecule	1227:1247	the acceptor molecule	1227:1247	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	6	31	theme	features	1002:1009	arg1	acceptors					970:978	glycosyl acceptors	961:978	glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	961:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	2	32	from	remarkable	303:312	arg1	terms					317:321	terms	317:321	terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications	317:419	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	6	33	theme	varying	983:989	arg1	N-acetylgalactosamine					1042:1062	N-acetylgalactosamine	1042:1062	N-acetylgalactosamine	1042:1062	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	galactose					1065:1073	galactose	1065:1073	galactose	1065:1073	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	acid					1113:1116	glucuronic acid	1102:1116	glucuronic acid	1102:1116	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	N-acetylglucosamine					1012:1030	N-acetylglucosamine	1012:1030	N-acetylglucosamine	1012:1030	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	glucose					1033:1039	glucose	1033:1039	glucose	1033:1039	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	acid					1092:1095	N-acetylmuramic acid	1076:1095	N-acetylmuramic acid	1076:1095	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	33	theme	varying	983:989	arg1	features					1002:1009	varying structural features	983:1009	varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	983:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	14	34	theme	acquired	2079:2086	arg1	data					2101:2104	the acquired experimental data	2075:2104	the acquired experimental data	2075:2104	In order to explain these findings and predict enzyme behavior, a modeling study was accomplished that correlated with the acquired experimental data.					
31817903	6	35	theme	acceptors	970:978	arg1	panel					952:956	a panel	950:956	a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	950:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	35	theme	acceptors	970:978	arg1	donor					851:855	an activated glycosyl donor	829:855	an activated glycosyl donor	829:855	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	35	theme	acceptors	970:978	arg1	N-acetyl-β-d-glucosaminide					872:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	35	theme	acceptors	970:978	arg1	N-acetyl-β-d-galactosaminide					916:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	36	theme	4-nitrophenyl	902:914	arg1	donor					851:855	an activated glycosyl donor	829:855	an activated glycosyl donor	829:855	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	36	theme	4-nitrophenyl	902:914	arg1	N-acetyl-β-d-galactosaminide					916:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	11	37	theme	N-acetylmuramic	1715:1729	arg1	acid					1731:1734	N-acetylmuramic acid	1715:1734	N-acetylmuramic acid	1715:1734	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	2	38	theme	specificity	367:377	arg1	terms					317:321	terms	317:321	terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications	317:419	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	13	39	gly	glycosylation	1903:1915	arg1	derivative					1944:1953	an N-acetylmuramic acid derivative	1920:1953	an N-acetylmuramic acid derivative	1920:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	13	40	theme	N-acetylmuramic	1923:1937	arg1	derivative					1944:1953	an N-acetylmuramic acid derivative	1920:1953	an N-acetylmuramic acid derivative	1920:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	9	41	theme	C-2	1326:1328	arg1	moiety					1339:1344	the C-2 hydroxyl moiety	1322:1344	the C-2 hydroxyl moiety	1322:1344	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	6	42	theme	N-acetylmuramic	1076:1090	arg1	acid					1092:1095	N-acetylmuramic acid	1076:1095	N-acetylmuramic acid	1076:1095	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	42	theme	N-acetylmuramic	1076:1090	arg1	features					1002:1009	varying structural features	983:1009	varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	983:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	13	43	theme	enzymatic	1893:1901	arg1	glycosylation					1903:1915	enzymatic glycosylation	1893:1915	enzymatic glycosylation of an N-acetylmuramic acid derivative	1893:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	9	44	theme	moiety	1339:1344	arg1	presence					1310:1317	The presence	1306:1317	The presence of the C-2 hydroxyl moiety	1306:1344	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	2	45	dep	Talaromyces	281:291	arg1	flavus					293:298	Talaromyces flavus	281:298	Talaromyces flavus	281:298	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	5	46	theme	β-N-acetylhexosaminidase	671:694	arg1	requirements					644:655	the structural requirements	629:655	the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors	629:731	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	11	47	theme	acid	1706:1709	arg1	β-glycosides					1683:1694	β-glycosides	1683:1694	β-glycosides of uronic acid and N-acetylmuramic acid	1683:1734	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	2	48	theme	broad	351:355	arg1	specificity					367:377	broad substrate specificity	351:377	broad substrate specificity	351:377	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	6	49	theme	4-nitrophenyl	858:870	arg1	donor					851:855	an activated glycosyl donor	829:855	an activated glycosyl donor	829:855	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	49	theme	4-nitrophenyl	858:870	arg1	N-acetyl-β-d-glucosaminide					872:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	2	50	theme	potential	340:348	arg1	terms					317:321	terms	317:321	terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications	317:419	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	1	51	theme	oligosaccharides	206:221	arg1	preparation					191:201	the preparation	187:201	the preparation of oligosaccharides of biological interest	187:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	6	52	theme	glucuronic	1102:1111	arg1	acid					1113:1116	glucuronic acid	1102:1116	glucuronic acid	1102:1116	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	52	theme	glucuronic	1102:1111	arg1	features					1002:1009	varying structural features	983:1009	varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	983:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	1	53	theme	biological	226:235	arg1	interest					237:244	biological interest	226:244	biological interest	226:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	1	54	theme	hydrolytic	139:148	arg1	enzymes					150:156	hydrolytic enzymes	139:156	hydrolytic enzymes in vivo	139:164	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	8	55	from	group	1218:1222	arg1	molecule					1240:1247	the acceptor molecule	1227:1247	the acceptor molecule	1227:1247	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	9	56	theme	normal	1368:1373	arg1	course					1375:1380	the normal course	1364:1380	the normal course of transglycosylation	1364:1402	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	5	57	theme	structure-activity	749:766	arg1	relationship					768:779	a structure-activity relationship study	747:785	a structure-activity relationship study	747:785	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	9	58	theme	unique	1414:1419	arg1	disaccharides					1434:1446	unique non-reducing disaccharides	1414:1446	unique non-reducing disaccharides	1414:1446	The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield.					
31817903	5	59	theme	structural	633:642	arg1	requirements					644:655	the structural requirements	629:655	the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors	629:731	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	1	60	theme	in	158:159	arg1	enzymes					150:156	hydrolytic enzymes	139:156	hydrolytic enzymes in vivo	139:164	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	6	61	theme	activated	832:840	arg1	donor					851:855	an activated glycosyl donor	829:855	an activated glycosyl donor	829:855	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	61	theme	activated	832:840	arg1	N-acetyl-β-d-glucosaminide					872:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	61	theme	activated	832:840	arg1	N-acetyl-β-d-galactosaminide					916:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	0	62	theme	Rational	76:83	arg1	Explanation					85:95	A Rational Explanation	74:95	A Rational Explanation	74:95	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	1	63	theme	useful	171:176	arg1	β-N-acetylhexosaminidases					105:129	Fungal β-N-acetylhexosaminidases	98:129	Fungal β-N-acetylhexosaminidases	98:129	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	1	63	theme	useful	171:176	arg1	tools					178:182	useful tools	171:182	useful tools in the preparation of oligosaccharides of biological interest	171:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	11	64	theme	mutant	1634:1639	arg1	enzyme					1641:1646	The Y470H mutant enzyme	1624:1646	The Y470H mutant enzyme	1624:1646	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	8	65	theme	C-2	1202:1204	arg1	group					1218:1222	The C-2 N-acetamido group	1198:1222	The C-2 N-acetamido group in the acceptor molecule	1198:1247	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	8	65	theme	C-2	1202:1204	arg1	essential					1265:1273	essential	1265:1273	essential	1265:1273	The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme.					
31817903	1	66	from	tools	178:182	arg1	preparation					191:201	the preparation	187:201	the preparation of oligosaccharides of biological interest	187:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	5	67	dep	relationship	768:779	arg1	study					781:785	study	781:785	study	781:785	The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study.					
31817903	0	68	from	Talaromyces	54:64	arg1	Specificity					9:19	Acceptor Specificity	0:19	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus	0:71	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	3	69	theme	high	480:483	arg1	yield					485:489	a high yield	478:489	a high yield	478:489	It can be heterologously produced in Pichia pastoris in a high yield.					
31817903	1	70	theme	Fungal	98:103	arg1	β-N-acetylhexosaminidases					105:129	Fungal β-N-acetylhexosaminidases	98:129	Fungal β-N-acetylhexosaminidases	98:129	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	1	70	theme	Fungal	98:103	arg1	tools					178:182	useful tools	171:182	useful tools in the preparation of oligosaccharides of biological interest	171:244	Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest.					
31817903	10	71	from	C-4	1509:1511	arg1	gluco-configuration					1486:1504	the gluco-configuration	1482:1504	the gluco-configuration at C-4	1482:1511	Moreover, whereas the gluco-configuration at C-4 steered the glycosylation into the β(1-4) position, the galacto-acceptor afforded a β(1-6) glycosidic linkage.					
31817903	13	72	theme	derivative	1944:1953	arg1	glycosylation					1903:1915	enzymatic glycosylation	1893:1915	enzymatic glycosylation of an N-acetylmuramic acid derivative	1893:1953	To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative.					
31817903	0	73	theme	β-N-Acetylhexosaminidase	24:47	arg1	Specificity					9:19	Acceptor Specificity	0:19	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus	0:71	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	2	74	from	Talaromyces	281:291	arg1	β-N-acetylhexosaminidase					251:274	The β-N-acetylhexosaminidase	247:274	The β-N-acetylhexosaminidase from Talaromyces flavus	247:298	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	2	74	from	Talaromyces	281:291	arg1	remarkable					303:312	remarkable	303:312	remarkable	303:312	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	6	75	theme	structural	991:1000	arg1	N-acetylgalactosamine					1042:1062	N-acetylgalactosamine	1042:1062	N-acetylgalactosamine	1042:1062	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	galactose					1065:1073	galactose	1065:1073	galactose	1065:1073	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	acid					1113:1116	glucuronic acid	1102:1116	glucuronic acid	1102:1116	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	N-acetylglucosamine					1012:1030	N-acetylglucosamine	1012:1030	N-acetylglucosamine	1012:1030	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	glucose					1033:1039	glucose	1033:1039	glucose	1033:1039	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	acid					1092:1095	N-acetylmuramic acid	1076:1095	N-acetylmuramic acid	1076:1095	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	75	theme	structural	991:1000	arg1	features					1002:1009	varying structural features	983:1009	varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	983:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	14	76	theme	experimental	2088:2099	arg1	data					2101:2104	the acquired experimental data	2075:2104	the acquired experimental data	2075:2104	In order to explain these findings and predict enzyme behavior, a modeling study was accomplished that correlated with the acquired experimental data.					
31817903	3	77	theme	Pichia	459:464	arg1	pastoris					466:473	Pichia pastoris	459:473	Pichia pastoris	459:473	It can be heterologously produced in Pichia pastoris in a high yield.					
31817903	4	78	theme	transglycosylation	561:578	arg1	capability					580:589	its transglycosylation capability	557:589	its transglycosylation capability	557:589	The mutation of the Tyr470 residue to histidine greatly enhances its transglycosylation capability.					
31817903	11	79	theme	acid	1731:1734	arg1	β-glycosides					1683:1694	β-glycosides	1683:1694	β-glycosides of uronic acid and N-acetylmuramic acid	1683:1734	The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid.					
31817903	0	80	dep	Talaromyces	54:64	arg1	flavus					66:71	Talaromyces flavus	54:71	Talaromyces flavus	54:71	Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation.					
31817903	4	81	theme	residue	519:525	arg1	mutation					496:503	The mutation	492:503	The mutation of the Tyr470 residue to histidine	492:538	The mutation of the Tyr470 residue to histidine greatly enhances its transglycosylation capability.					
31817903	6	82	dep	features	1002:1009	arg1	N-acetylgalactosamine					1042:1062	N-acetylgalactosamine	1042:1062	N-acetylgalactosamine	1042:1062	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	galactose					1065:1073	galactose	1065:1073	galactose	1065:1073	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	acid					1113:1116	glucuronic acid	1102:1116	glucuronic acid	1102:1116	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	N-acetylglucosamine					1012:1030	N-acetylglucosamine	1012:1030	N-acetylglucosamine	1012:1030	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	glucose					1033:1039	glucose	1033:1039	glucose	1033:1039	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	acid					1092:1095	N-acetylmuramic acid	1076:1095	N-acetylmuramic acid	1076:1095	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	82	dep	features	1002:1009	arg1	features					1002:1009	varying structural features	983:1009	varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	983:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	83	theme	glycosyl	842:849	arg1	donor					851:855	an activated glycosyl donor	829:855	an activated glycosyl donor	829:855	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	83	theme	glycosyl	842:849	arg1	N-acetyl-β-d-glucosaminide					872:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	4-nitrophenyl N-acetyl-β-d-glucosaminide	858:897	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	83	theme	glycosyl	842:849	arg1	N-acetyl-β-d-galactosaminide					916:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	4-nitrophenyl N-acetyl-β-d-galactosaminide	902:943	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	6	84	theme	glycosyl	961:968	arg1	acceptors					970:978	glycosyl acceptors	961:978	glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid)	961:1117	Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid).					
31817903	12	85	with	able	1771:1774	arg1	acceptor					1753:1760	the latter acceptor	1742:1760	the latter acceptor	1742:1760	With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield.					
31817903	2	86	theme	substrate	357:365	arg1	specificity					367:377	broad substrate specificity	351:377	broad substrate specificity	351:377	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
31817903	2	87	theme	substrate	397:405	arg1	modifications					407:419	substrate modifications	397:419	substrate modifications	397:419	The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications.					
30392563	5	0	theme	eye	758:760	arg1	cells					762:766	eye cells	758:766	eye cells	758:766	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	3	1	theme	sialylated	509:518	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	5	2	theme	cells	762:766	arg1	treatment					745:753	treatment	745:753	treatment of eye cells with TNFα	745:776	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	4	3	located	found	715:719	arg1	mucins					724:729	mucins	724:729	mucins	724:729	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	4	3	located	found	715:719	arg2	N-glycans					696:704	complex N-glycans	688:704	complex N-glycans that are found on mucins	688:729	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	1	4	theme	important	154:162	arg1	roles					164:168	important roles	154:168	important roles	154:168	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	2	5	theme	glycosyltransferase	328:346	arg1	activities					348:357	the glycosyltransferase activities	324:357	the glycosyltransferase activities that synthesize the O-glycans of mucins	324:397	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	2	6	theme	human	265:269	arg1	corneal					271:277	cultured human corneal and conjunctival epithelial cells	256:311	corneal	271:277	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	1	7	gly	glycoproteins	87:99	arg1	glycoproteins					87:99	Mucin glycoproteins	81:99	Mucin glycoproteins on the ocular surface	81:121	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	3	8	theme	core	548:551	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	1	9	from	O-glycans	135:143	arg1	rich					127:130	rich	127:130	rich	127:130	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	2	10	theme	cultured	256:263	arg1	corneal					271:277	cultured human corneal and conjunctival epithelial cells	256:311	corneal	271:277	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	5	11	theme	inflammatory	854:865	arg1	stimuli					867:873	inflammatory stimuli	854:873	inflammatory stimuli of the eye surface	854:892	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	4	12	theme	complex	688:694	arg1	N-glycans					696:704	complex N-glycans	688:704	complex N-glycans that are found on mucins	688:729	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	1	13	contain	have	149:152	arg2	roles					164:168	important roles	154:168	important roles	154:168	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	1	13	contain	have	149:152	arg1	glycoproteins					87:99	Mucin glycoproteins	81:99	Mucin glycoproteins on the ocular surface	81:121	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	5	14	theme	dynamic	831:837	arg1	fashion					839:845	a dynamic fashion	829:845	a dynamic fashion	829:845	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	3	15	theme	Galβ1-3GalNAcα	528:541	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	2	16	theme	mucins	392:397	arg1	O-glycans					379:387	the O-glycans	375:387	the O-glycans of mucins	375:397	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	1	17	from	rich	127:130	arg1	O-glycans					135:143	O-glycans	135:143	O-glycans	135:143	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	1	18	theme	Mucin	81:85	arg1	glycoproteins					87:99	Mucin glycoproteins	81:99	Mucin glycoproteins on the ocular surface	81:121	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	3	19	theme	epithelial	441:450	arg1	cells					452:456	ocular surface epithelial cells	426:456	ocular surface epithelial cells	426:456	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	5	20	theme	epithelial	791:800	arg1	O-glycosylation					802:816	epithelial O-glycosylation	791:816	epithelial O-glycosylation	791:816	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	2	21	theme	epithelial	296:305	arg1	cells					307:311	cultured human corneal and conjunctival epithelial cells	256:311	cells	307:311	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	0	22	theme	Glycosylation	0:12	arg1	pathways					14:21	Glycosylation pathways	0:21	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins	0:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	2	23	theme	conjunctival	283:294	arg1	cells					307:311	cultured human corneal and conjunctival epithelial cells	256:311	cells	307:311	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	3	24	theme	ocular	426:431	arg1	cells					452:456	ocular surface epithelial cells	426:456	ocular surface epithelial cells	426:456	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	0	25	theme	human	26:30	arg1	corneal					32:38	human corneal and conjunctival epithelial cell mucins	26:78	corneal	32:38	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	2	26	contain	have	251:254	arg2	cells					307:311	cultured human corneal and conjunctival epithelial cells	256:311	cells	307:311	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	2	26	contain	have	251:254	arg1	we					248:249	we	248:249	we	248:249	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	2	26	contain	have	251:254	arg2	corneal					271:277	cultured human corneal and conjunctival epithelial cells	256:311	corneal	271:277	In this work, we have cultured human corneal and conjunctival epithelial cells to examine the glycosyltransferase activities that synthesize the O-glycans of mucins.					
30392563	3	27	theme	active	463:468	arg1	enzymes					470:476	active enzymes	463:476	active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	463:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	3	28	contain	have	458:461	arg2	enzymes					470:476	active enzymes	463:476	active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	463:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	3	28	contain	have	458:461	arg1	cells					452:456	ocular surface epithelial cells	426:456	ocular surface epithelial cells	426:456	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	1	29	theme	ocular	108:113	arg1	surface					115:121	the ocular surface	104:121	the ocular surface	104:121	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	1	30	theme	physical	193:200	arg1	impact					226:231	physical, chemical and microbial impact	193:231	physical, chemical and microbial impact	193:231	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	3	31	theme	GalNAcα	575:581	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	4	32	theme	Eye	644:646	arg1	cells					648:652	Eye cells	644:652	Eye cells	644:652	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	0	33	theme	corneal	32:38	arg1	pathways					14:21	Glycosylation pathways	0:21	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins	0:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	3	34	theme	structural	624:633	arg1	studies					635:641	previous structural studies	615:641	previous structural studies	615:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	1	35	theme	chemical	203:210	arg1	impact					226:231	physical, chemical and microbial impact	193:231	physical, chemical and microbial impact	193:231	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	0	36	theme	epithelial	57:66	arg1	mucins					73:78	human corneal and conjunctival epithelial cell mucins	26:78	mucins	73:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	1	37	from	impact	226:231	arg1	protection					177:186	the protection	173:186	the protection from physical, chemical and microbial impact	173:231	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	3	38	theme	surface	433:439	arg1	cells					452:456	ocular surface epithelial cells	426:456	ocular surface epithelial cells	426:456	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	0	39	theme	conjunctival	44:55	arg1	mucins					73:78	human corneal and conjunctival epithelial cell mucins	26:78	mucins	73:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	4	40	contain	have	659:662	arg2	enzymes					664:670	enzymes	664:670	enzymes that synthesize complex N-glycans that are found on mucins	664:729	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	4	40	contain	have	659:662	arg1	cells					648:652	Eye cells	644:652	Eye cells	644:652	Eye cells also have enzymes that synthesize complex N-glycans that are found on mucins.					
30392563	3	41	theme	core	520:523	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	3	42	gly	sialylated	509:518	arg1	structures					583:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures	509:592	sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies	509:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	5	43	from	treatment	745:753	arg1	Results					732:738	Results	732:738	Results from treatment of eye cells with TNFα	732:776	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	3	44	theme	previous	615:622	arg1	studies					635:641	previous structural studies	615:641	previous structural studies	615:641	The results indicate that ocular surface epithelial cells have active enzymes that synthesize O-glycans with sialylated core 1, Galβ1-3GalNAcα, and core 2, GlcNAcβ1-6(Galβ1-3)GalNAcα structures which corresponds to previous structural studies.					
30392563	1	45	from	glycoproteins	87:99	arg1	surface					115:121	the ocular surface	104:121	the ocular surface	104:121	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	1	46	theme	microbial	216:224	arg1	impact					226:231	physical, chemical and microbial impact	193:231	physical, chemical and microbial impact	193:231	Mucin glycoproteins on the ocular surface are rich in O-glycans and have important roles in the protection from physical, chemical and microbial impact.					
30392563	0	47	theme	mucins	73:78	arg1	pathways					14:21	Glycosylation pathways	0:21	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins	0:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	5	48	with	treatment	745:753	arg1	TNFα					773:776	TNFα	773:776	TNFα	773:776	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	5	49	theme	eye	882:884	arg1	surface					886:892	the eye surface	878:892	the eye surface	878:892	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
30392563	0	50	theme	cell	68:71	arg1	mucins					73:78	human corneal and conjunctival epithelial cell mucins	26:78	mucins	73:78	Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.					
30392563	5	51	theme	surface	886:892	arg1	stimuli					867:873	inflammatory stimuli	854:873	inflammatory stimuli of the eye surface	854:892	Results from treatment of eye cells with TNFα suggest that epithelial O-glycosylation changes in a dynamic fashion during inflammatory stimuli of the eye surface.					
29931153	2	0	theme	glycans	578:584	arg1	glycans					578:584	glycans	578:584	glycans	578:584	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	2	0	theme	glycans	578:584	arg1	set					571:573	a certain set	561:573	a certain set of glycans	561:584	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	2	1	from	N-glycans	414:422	arg1	glycoproteins					427:439	glycoproteins	427:439	glycoproteins	427:439	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	1	2	from	enzymes	330:336	arg1	ER					345:346	ER	345:346	ER	345:346	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	2	from	enzymes	330:336	arg1	Golgi					352:356	Golgi	352:356	Golgi	352:356	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	2	3	dep	ER	533:534	arg1	the					529:531	the	529:531	the	529:531	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	9	4	gly	glycoproteins	1652:1664	arg1	glycoproteins					1652:1664	immature glycoproteins	1643:1664	immature glycoproteins	1643:1664	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	6	5	theme	N-glycan	1281:1288	arg1	accessibility					1213:1225	the solvent accessibility	1201:1225	the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan	1201:1288	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	4	6	theme	other	827:831	arg1	hand					833:836	the other hand	823:836	the other hand	823:836	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	7	7	theme	glycoproteins	1435:1447	arg1	circles					1411:1417	concentric circles	1400:1417	concentric circles of fully folded glycoproteins	1400:1447	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	6	8	gly	glycosylated	1234:1245	arg1	residue					1251:1257	the glycosylated Asn residue	1230:1257	the glycosylated Asn residue	1230:1257	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	2	9	theme	Golgi	540:544	arg1	apparatus					546:554	Golgi apparatus	540:554	Golgi apparatus	540:554	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	6	10	theme	site-specific	1143:1155	arg1	processing					1166:1175	site-specific N-glycan processing	1143:1175	site-specific N-glycan processing	1143:1175	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	4	11	gly	glycoprotein	888:899	arg1	glycoprotein					888:899	the carrier glycoprotein	876:899	the carrier glycoprotein	876:899	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	4	12	theme	N-glycan	904:911	arg1	maturation					913:922	N-glycan maturation	904:922	N-glycan maturation	904:922	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	2	13	from	hybrid	484:489	arg1	mammals					512:518	mammals	512:518	mammals	512:518	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	6	14	theme	residue	1251:1257	arg1	accessibility					1213:1225	the solvent accessibility	1201:1225	the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan	1201:1288	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	0	15	theme	processing	141:150	arg1	basis					117:121	the structural basis	102:121	the structural basis of N-glycoprotein processing and maturation	102:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	4	16	theme	structure	863:871	arg1	influence					843:851	the influence	839:851	the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation	839:922	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	6	17	theme	glycosylated	1234:1245	arg1	residue					1251:1257	the glycosylated Asn residue	1230:1257	the glycosylated Asn residue	1230:1257	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	3	18	theme	N-glycoprotein	725:738	arg1	maturation					740:749	N-glycoprotein maturation	725:749	N-glycoprotein maturation	725:749	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	0	19	theme	maturation	156:165	arg1	basis					117:121	the structural basis	102:121	the structural basis of N-glycoprotein processing and maturation	102:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	0	20	gly	N-glycoprotein	126:139	arg1	N-glycoprotein					126:139	N-glycoprotein processing	126:150	N-glycoprotein processing	126:150	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	3	21	theme	expression	764:773	arg1	levels					775:780	the expression levels	760:780	the expression levels of glycosyltransferases/glycosidases	760:817	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	8	22	theme	acid	1488:1491	arg1	composition					1493:1503	amino acid composition	1482:1503	amino acid composition	1482:1503	There does appear to be bias in amino acid composition and secondary structure.					
29931153	6	23	theme	solvent	1205:1211	arg1	accessibility					1213:1225	the solvent accessibility	1201:1225	the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan	1201:1288	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	4	24	theme	model	970:974	arg1	glycoproteins					976:988	individual model glycoproteins	959:988	individual model glycoproteins	959:988	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	4	25	from	influence	843:851	arg1	maturation					913:922	N-glycan maturation	904:922	N-glycan maturation	904:922	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	9	26	theme	γ-branched	1544:1553	arg1	residues					1566:1573	γ-branched amino acid residues	1544:1573	γ-branched amino acid residues (Asn+Asp+Leu)	1544:1587	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	9	26	theme	γ-branched	1544:1553	arg1	Asn+Asp+Leu					1576:1586	Asn+Asp+Leu	1576:1586	Asn+Asp+Leu	1576:1586	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	2	27	dep	form	623:626	arg1	type					642:645	high-mannose type	629:645	high-mannose type in mammals	629:656	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	3	28	gly	N-glycoprotein	725:738	arg1	N-glycoprotein					725:738	N-glycoprotein maturation	725:749	N-glycoprotein maturation	725:749	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	2	29	theme	"	621:621	arg1	form					623:626	an "immature" form	609:626	an "immature" form (high-mannose type in mammals)	609:657	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	2	30	dep	forms	477:481	arg1	hybrid					484:489	hybrid	484:489	hybrid	484:489	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	2	30	dep	forms	477:481	arg1	types					503:507	complex types	495:507	complex types	495:507	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	9	31	theme	acid	1561:1564	arg1	residues					1566:1573	γ-branched amino acid residues	1544:1573	γ-branched amino acid residues (Asn+Asp+Leu)	1544:1587	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	9	31	theme	acid	1561:1564	arg1	Asn+Asp+Leu					1576:1586	Asn+Asp+Leu	1576:1586	Asn+Asp+Leu	1576:1586	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	10	32	theme	surface	1702:1708	arg1	features					1678:1685	Structural features	1667:1685	Structural features of the protein surface around the N-glycosylated site	1667:1739	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	0	33	theme	structural	106:115	arg1	basis					117:121	the structural basis	102:121	the structural basis of N-glycoprotein processing and maturation	102:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	5	34	theme	Protein	1108:1114	arg1	Bank					1121:1124	the Protein Data Bank	1104:1124	the Protein Data Bank	1104:1124	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	1	35	from	ER	345:346	arg1	set					312:314	a set	310:314	a set of processing enzymes in the ER and Golgi apparatus	310:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	35	from	ER	345:346	arg1	enzymes					330:336	processing enzymes	319:336	processing enzymes in the ER and Golgi apparatus	319:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	7	36	theme	mature	1356:1361	arg1	forms					1363:1367	immature or mature forms	1344:1367	immature or mature forms	1344:1367	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	8	37	from	bias	1474:1477	arg1	structure					1519:1527	secondary structure	1509:1527	secondary structure	1509:1527	There does appear to be bias in amino acid composition and secondary structure.					
29931153	8	37	from	bias	1474:1477	arg1	composition					1493:1503	amino acid composition	1482:1503	amino acid composition	1482:1503	There does appear to be bias in amino acid composition and secondary structure.					
29931153	10	38	theme	Structural	1667:1676	arg1	features					1678:1685	Structural features	1667:1685	Structural features of the protein surface around the N-glycosylated site	1667:1739	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	7	39	theme	immature	1344:1351	arg1	forms					1363:1367	immature or mature forms	1344:1367	immature or mature forms	1344:1367	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	5	40	gly	N-glycoprotein	1020:1033	arg1	N-glycoprotein					1020:1033	N-glycoprotein maturation	1020:1044	N-glycoprotein maturation	1020:1044	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	1	41	link	Asparagine-linked	168:184	arg1	N-glycans					195:203	N-glycans	195:203	N-glycans	195:203	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	41	link	Asparagine-linked	168:184	arg1	glycans					186:192	Asparagine-linked glycans	168:192	Asparagine-linked glycans (N-glycans)	168:204	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	0	42	theme	protein	12:18	arg1	Analysis					0:7	Analysis	0:7	Analysis of protein	0:18	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	7	43	theme	features	1328:1335	arg1	bias					1301:1304	Potential bias	1291:1304	Potential bias of protein structural features toward immature or mature forms	1291:1367	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	10	44	theme	protein	1694:1700	arg1	surface					1702:1708	the protein surface	1690:1708	the protein surface around the N-glycosylated site	1690:1739	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	7	45	theme	protein	1309:1315	arg1	features					1328:1335	protein structural features	1309:1335	protein structural features	1309:1335	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	2	46	theme	mature	470:475	arg1	forms					477:481	mature forms	470:481	mature forms (hybrid and complex types in mammals)	470:519	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	1	47	theme	processing	319:328	arg1	enzymes					330:336	processing enzymes	319:336	processing enzymes in the ER and Golgi apparatus	319:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	5	48	theme	N-glycoprotein	1020:1033	arg1	maturation					1035:1044	N-glycoprotein maturation	1020:1044	N-glycoprotein maturation	1020:1044	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	5	49	gly	glycoprotein	1065:1076	arg1	glycoprotein					1065:1076	glycoprotein structures	1065:1087	glycoprotein structures extracted from the Protein Data Bank	1065:1124	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	10	50	theme	N-glycosylated	1721:1734	arg1	site					1736:1739	the N-glycosylated site	1717:1739	the N-glycosylated site	1717:1739	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	0	51	gly	N-glycosylation	38:52	arg2	sites					54:58	N-glycosylation sites	38:58	N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation	38:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	2	52	theme	Accumulating	369:380	arg1	evidence					382:389	Accumulating evidence	369:389	Accumulating evidence	369:389	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	1	53	theme	endoplasmic	253:263	arg1	ER					276:277	ER	276:277	ER	276:277	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	53	theme	endoplasmic	253:263	arg1	reticulum					265:273	the endoplasmic reticulum	249:273	the endoplasmic reticulum (ER)	249:278	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	9	54	theme	glycoproteins	1652:1664	arg1	regions					1632:1638	unstructured loop regions	1614:1638	unstructured loop regions of immature glycoproteins	1614:1664	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	3	55	theme	Much	660:663	arg1	attention					665:673	Much attention	660:673	Much attention	660:673	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	1	56	from	set	312:314	arg1	ER					345:346	ER	345:346	ER	345:346	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	56	from	set	312:314	arg1	Golgi					352:356	Golgi	352:356	Golgi	352:356	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	2	57	theme	certain	563:569	arg1	glycans					578:584	glycans	578:584	glycans	578:584	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	2	57	theme	certain	563:569	arg1	set					571:573	a certain set	561:573	a certain set of glycans	561:584	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	6	58	theme	conjugated	1270:1279	arg1	N-glycan					1281:1288	the conjugated N-glycan	1266:1288	the conjugated N-glycan	1266:1288	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	7	59	gly	glycoproteins	1435:1447	arg1	glycoproteins					1435:1447	fully folded glycoproteins	1422:1447	fully folded glycoproteins	1422:1447	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	1	60	gly	glycoproteins	232:244	arg1	glycoproteins					232:244	nascent glycoproteins	224:244	nascent glycoproteins in the endoplasmic reticulum (ER)	224:278	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	7	61	theme	folded	1428:1433	arg1	glycoproteins					1435:1447	fully folded glycoproteins	1422:1447	fully folded glycoproteins	1422:1447	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	0	62	theme	N-glycoprotein	126:139	arg1	processing					141:150	N-glycoprotein processing	126:150	N-glycoprotein processing	126:150	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	9	63	theme	loop	1627:1630	arg1	regions					1632:1638	unstructured loop regions	1614:1638	unstructured loop regions of immature glycoproteins	1614:1664	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	6	64	theme	N-glycan	1157:1164	arg1	processing					1166:1175	site-specific N-glycan processing	1143:1175	site-specific N-glycan processing	1143:1175	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	4	65	theme	carrier	880:886	arg1	glycoprotein					888:899	the carrier glycoprotein	876:899	the carrier glycoprotein	876:899	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	3	66	theme	environmental	692:704	arg1	factors					706:712	environmental factors	692:712	environmental factors	692:712	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	3	66	theme	environmental	692:704	arg1	levels					775:780	the expression levels	760:780	the expression levels of glycosyltransferases/glycosidases	760:817	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	5	67	theme	glycoprotein	1065:1076	arg1	structures					1078:1087	glycoprotein structures	1065:1087	glycoprotein structures extracted from the Protein Data Bank	1065:1124	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	1	68	theme	nascent	224:230	arg1	glycoproteins					232:244	nascent glycoproteins	224:244	nascent glycoproteins in the endoplasmic reticulum (ER)	224:278	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	2	69	from	types	503:507	arg1	mammals					512:518	mammals	512:518	mammals	512:518	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	6	70	theme	Asn	1247:1249	arg1	residue					1251:1257	the glycosylated Asn residue	1230:1257	the glycosylated Asn residue	1230:1257	We confirm that site-specific N-glycan processing is largely explained by the solvent accessibility of the glycosylated Asn residue and of the conjugated N-glycan.					
29931153	1	71	from	glycoproteins	232:244	arg1	ER					276:277	ER	276:277	ER	276:277	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	71	from	glycoproteins	232:244	arg1	reticulum					265:273	the endoplasmic reticulum	249:273	the endoplasmic reticulum (ER)	249:278	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	4	72	theme	3D	860:861	arg1	structure					863:871	the 3D structure	856:871	the 3D structure of the carrier glycoprotein	856:899	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	8	73	theme	amino	1482:1486	arg1	composition					1493:1503	amino acid composition	1482:1503	amino acid composition	1482:1503	There does appear to be bias in amino acid composition and secondary structure.					
29931153	7	74	theme	circles	1411:1417	arg1	range					1391:1395	a range	1389:1395	a range of concentric circles of fully folded glycoproteins	1389:1447	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	0	75	theme	PDB	69:71	arg1	repository					73:82	the PDB repository	65:82	the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation	65:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	2	76	theme	complex	495:501	arg1	types					503:507	complex types	495:507	complex types	495:507	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	8	77	theme	secondary	1509:1517	arg1	structure					1519:1527	secondary structure	1509:1527	secondary structure	1509:1527	There does appear to be bias in amino acid composition and secondary structure.					
29931153	1	78	theme	Asparagine-linked	168:184	arg1	N-glycans					195:203	N-glycans	195:203	N-glycans	195:203	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	78	theme	Asparagine-linked	168:184	arg1	glycans					186:192	Asparagine-linked glycans	168:192	Asparagine-linked glycans (N-glycans)	168:204	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	4	79	theme	individual	959:968	arg1	glycoproteins					976:988	individual model glycoproteins	959:988	individual model glycoproteins	959:988	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	2	80	from	type	642:645	arg1	mammals					650:656	mammals	650:656	mammals	650:656	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	4	81	theme	glycoprotein	888:899	arg1	structure					863:871	the 3D structure	856:871	the 3D structure of the carrier glycoprotein	856:899	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	9	82	theme	amino	1555:1559	arg1	residues					1566:1573	γ-branched amino acid residues	1544:1573	γ-branched amino acid residues (Asn+Asp+Leu)	1544:1587	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	9	82	theme	amino	1555:1559	arg1	Asn+Asp+Leu					1576:1586	Asn+Asp+Leu	1576:1586	Asn+Asp+Leu	1576:1586	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	2	83	theme	immature	613:620	arg1	form					623:626	an "immature" form	609:626	an "immature" form (high-mannose type in mammals)	609:657	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	3	84	theme	glycosyltransferases/glycosidases	785:817	arg1	levels					775:780	the expression levels	760:780	the expression levels of glycosyltransferases/glycosidases	760:817	Much attention has been paid to environmental factors regulating N-glycoprotein maturation, such as the expression levels of glycosyltransferases/glycosidases.					
29931153	5	85	theme	Data	1116:1119	arg1	Bank					1121:1124	the Protein Data Bank	1104:1124	the Protein Data Bank	1104:1124	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	1	86	dep	ER	345:346	arg1	apparatus					358:366	apparatus	358:366	apparatus	358:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	86	dep	ER	345:346	arg1	the					341:343	the	341:343	the	341:343	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	2	87	theme	high-mannose	629:640	arg1	type					642:645	high-mannose type	629:645	high-mannose type in mammals	629:656	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29931153	0	88	from	repository	73:82	arg1	sites					54:58	N-glycosylation sites	38:58	N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation	38:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	1	89	from	Golgi	352:356	arg1	set					312:314	a set	310:314	a set of processing enzymes in the ER and Golgi apparatus	310:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	89	from	Golgi	352:356	arg1	enzymes					330:336	processing enzymes	319:336	processing enzymes in the ER and Golgi apparatus	319:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	9	90	theme	immature	1643:1650	arg1	glycoproteins					1652:1664	immature glycoproteins	1643:1664	immature glycoproteins	1643:1664	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	7	91	theme	structural	1317:1326	arg1	features					1328:1335	protein structural features	1309:1335	protein structural features	1309:1335	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	10	92	theme	N-glycan	1759:1766	arg1	processing					1768:1777	N-glycan processing	1759:1777	N-glycan processing	1759:1777	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	0	93	theme	N-glycosylation	38:52	arg1	sites					54:58	N-glycosylation sites	38:58	N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation	38:165	Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation.					
29931153	5	94	theme	more	1001:1004	arg1	insights					1006:1013	more insights	1001:1013	more insights into N-glycoprotein maturation	1001:1044	To obtain more insights into N-glycoprotein maturation, we herein analyze glycoprotein structures extracted from the Protein Data Bank.					
29931153	9	95	theme	unstructured	1614:1625	arg1	regions					1632:1638	unstructured loop regions	1614:1638	unstructured loop regions of immature glycoproteins	1614:1664	Most notably, γ-branched amino acid residues (Asn+Asp+Leu) occur more frequently on unstructured loop regions of immature glycoproteins.					
29931153	7	96	theme	Potential	1291:1299	arg1	bias					1301:1304	Potential bias	1291:1304	Potential bias of protein structural features toward immature or mature forms	1291:1367	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	1	97	theme	enzymes	330:336	arg1	set					312:314	a set	310:314	a set of processing enzymes in the ER and Golgi apparatus	310:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	1	97	theme	enzymes	330:336	arg1	enzymes					330:336	processing enzymes	319:336	processing enzymes in the ER and Golgi apparatus	319:366	Asparagine-linked glycans (N-glycans) are attached onto nascent glycoproteins in the endoplasmic reticulum (ER) and subsequently processed by a set of processing enzymes in the ER and Golgi apparatus.					
29931153	4	98	gly	glycoproteins	976:988	arg1	glycoproteins					976:988	individual model glycoproteins	959:988	individual model glycoproteins	959:988	On the other hand, the influence of the 3D structure of the carrier glycoprotein on N-glycan maturation has been investigated mostly using individual model glycoproteins.					
29931153	7	99	theme	concentric	1400:1409	arg1	circles					1411:1417	concentric circles	1400:1417	concentric circles of fully folded glycoproteins	1400:1447	Potential bias of protein structural features toward immature or mature forms was explored within a range of concentric circles of fully folded glycoproteins.					
29931153	10	100	gly	N-glycosylated	1721:1734	arg1	site					1736:1739	the N-glycosylated site	1717:1739	the N-glycosylated site	1717:1739	Structural features of the protein surface around the N-glycosylated site do seem to affect N-glycan processing and maturation.					
29931153	2	101	gly	glycoproteins	427:439	arg1	glycoproteins					427:439	glycoproteins	427:439	glycoproteins	427:439	Accumulating evidence has shown that not all N-glycans on glycoproteins are uniformly processed into mature forms (hybrid and complex types in mammals) through the ER and Golgi apparatus, and a certain set of glycans remains unprocessed as an "immature" form (high-mannose type in mammals).					
29885470	12	0	theme	biological	2265:2274	arg1	activities					2276:2285	many biological activities	2260:2285	many biological activities	2260:2285	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	6	1	theme	de-N-acetylation	991:1006	arg1	degradation					925:935	peeling degradation	917:935	peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation	917:1006	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	6	2	theme	acidic	865:870	arg1	N-glycans					872:880	the neutral and acidic N-glycans	849:880	the neutral and acidic N-glycans	849:880	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	14	3	theme	N-glycan	2721:2728	arg1	biomarkers					2730:2739	N-glycan biomarkers	2721:2739	N-glycan biomarkers of some major diseases	2721:2762	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	10	4	from	activities	1871:1880	arg1	regulation					1826:1835	the structural and functional regulation	1796:1835	the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases	1796:1927	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	14	5	theme	structure-function	2646:2663	arg1	relationship					2665:2676	the complicated structure-function relationship	2630:2676	the complicated structure-function relationship of N-glycans	2630:2689	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	10	6	theme	post-translational	1731:1748	arg1	modification					1750:1761	the most common post-translational modification	1715:1761	the most common post-translational modification forms	1715:1767	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	6	7	theme	neutral	853:859	arg1	N-glycans					872:880	the neutral and acidic N-glycans	849:880	the neutral and acidic N-glycans	849:880	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	10	8	theme	human	1903:1907	arg1	health					1909:1914	human health	1903:1914	human health	1903:1914	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	12	9	theme	functional	2235:2244	arg1	mechanisms					2246:2255	the functional mechanisms	2231:2255	the functional mechanisms of many biological activities	2231:2285	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	6	10	theme	α-1,3-fucosylated	949:965	arg1	structure					967:975	the core α-1,3-fucosylated structure	940:975	the core α-1,3-fucosylated structure	940:975	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	11	11	theme	corresponding	2083:2095	arg1	receptors					2105:2113	their corresponding protein receptors	2077:2113	their corresponding protein receptors	2077:2113	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	12	12	theme	different	2171:2179	arg1	types					2181:2185	different types	2171:2185	different types of N-glycans	2171:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	1	13	theme	biological	188:197	arg1	processes					199:207	biological processes	188:207	biological processes	188:207	Glycoproteins play pivotal roles in a series of biological processes and their glycosylation patterns need to be structurally and functionally characterized.					
29885470	8	14	theme	quaternary	1302:1311	arg1	cations					1322:1328	quaternary ammonium cations	1302:1328	quaternary ammonium cations	1302:1328	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	14	15	theme	biomarkers	2730:2739	arg1	search					2711:2716	the search	2707:2716	the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs	2707:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	9	16	theme	branches	1600:1607	arg1	determination					1583:1595	determination	1583:1595	determination of branches and linkages	1583:1620	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	14	17	theme	complicated	2634:2644	arg1	relationship					2665:2676	the complicated structure-function relationship	2630:2676	the complicated structure-function relationship of N-glycans	2630:2689	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	10	18	theme	forms	1763:1767	arg1	forms					1763:1767	the most common post-translational modification forms	1715:1767	the most common post-translational modification forms	1715:1767	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	10	18	theme	forms	1763:1767	arg1	one					1708:1710	one	1708:1710	one	1708:1710	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	13	19	theme	simple	2344:2349	arg1	method					2362:2367	a simple, versatile method	2342:2367	a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions	2342:2477	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	2	20	theme	versatile	319:327	arg1	methods					329:335	versatile methods	319:335	versatile methods	319:335	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	9	21	theme	linkages	1613:1620	arg1	determination					1583:1595	determination	1583:1595	determination of branches and linkages	1583:1620	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	9	22	theme	sequential	1625:1634	arg1	fragmentation					1640:1652	sequential MSn fragmentation	1625:1652	sequential MSn fragmentation	1625:1652	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	12	23	theme	types	2181:2185	arg1	characterization					2151:2166	Detailed structural and functional characterization	2116:2166	Detailed structural and functional characterization of different types of N-glycans	2116:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	6	24	theme	general	1023:1029	arg1	applicability					1031:1043	its general applicability	1019:1043	its general applicability to various types of N-glycans	1019:1073	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	12	25	theme	many	2310:2313	arg1	diseases					2315:2322	many diseases	2310:2322	many diseases	2310:2322	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	11	26	theme	glycoproteins	1984:1996	arg1	N-glycans					1971:1979	the N-glycans	1967:1979	the N-glycans of glycoproteins	1967:1996	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	7	27	theme	esculentum	1149:1158	arg1	Pollen					1167:1172	Fagopyrum esculentum Moench Pollen	1139:1172	Fagopyrum esculentum Moench Pollen	1139:1172	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	7	28	theme	online	1212:1217	arg1	LC/MS					1219:1223	online LC/MS	1212:1223	online LC/MS	1212:1223	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	8	29	theme	multi-stage	1419:1429	arg1	spectrometry					1436:1447	multi-stage mass spectrometry	1419:1447	sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing	1408:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	8	29	theme	multi-stage	1419:1429	arg1	MSn					1450:1452	MSn	1450:1452	MSn	1450:1452	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	1	30	theme	pivotal	159:165	arg1	roles					167:171	pivotal roles	159:171	pivotal roles	159:171	Glycoproteins play pivotal roles in a series of biological processes and their glycosylation patterns need to be structurally and functionally characterized.					
29885470	8	31	theme	spectrometry	1436:1447	arg1	fragmentation					1455:1467	sequential multi-stage mass spectrometry (MSn) fragmentation	1408:1467	sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing	1408:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	5	32	theme	online	817:822	arg1	LC/MS					824:828	online LC/MS	817:828	online LC/MS	817:828	The released glycans are conveniently labeled with 9-fluorenylmethyloxycarbonyl (Fmoc) and analyzed by ESI-MS and online LC/MS.					
29885470	11	33	theme	informational	1943:1955	arg1	molecule					1957:1964	informational molecule	1943:1964	informational molecule	1943:1964	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	12	34	theme	Detailed	2116:2123	arg1	characterization					2151:2166	Detailed structural and functional characterization	2116:2166	Detailed structural and functional characterization of different types of N-glycans	2116:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	12	35	theme	diseases	2315:2322	arg1	pathologies					2295:2305	the pathologies	2291:2305	the pathologies of many diseases	2291:2322	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	12	35	theme	diseases	2315:2322	arg1	mechanisms					2246:2255	the functional mechanisms	2231:2255	the functional mechanisms of many biological activities	2231:2285	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	6	36	theme	N-glycans	1065:1073	arg1	types					1056:1060	various types	1048:1060	various types of N-glycans	1048:1073	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	7	37	attach	derived	1107:1113	arg1	Pollen					1167:1172	Fagopyrum esculentum Moench Pollen	1139:1172	Fagopyrum esculentum Moench Pollen	1139:1172	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	7	37	attach	derived	1107:1113	arg1	ovalbumin					1128:1136	chicken ovalbumin	1120:1136	chicken ovalbumin	1120:1136	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	7	37	attach	derived	1107:1113	arg2	N-glycans					1097:1105	The Fmoc-derivatized N-glycans	1076:1105	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS	1076:1180	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	7	37	attach	derived	1107:1113	arg1	FBS					1178:1180	FBS	1178:1180	FBS	1178:1180	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	8	38	theme	glycan	1473:1478	arg1	sequencing					1480:1489	glycan sequencing	1473:1489	glycan sequencing	1473:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	0	39	theme	LC/MS	124:128	arg1	analysis					130:137	LC/MS analysis	124:137	LC/MS analysis	124:137	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	10	40	theme	functional	1815:1824	arg1	regulation					1826:1835	the structural and functional regulation	1796:1835	the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases	1796:1927	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	0	41	theme	Reductive	0:8	arg1	release					19:25	Reductive chemical release	0:25	Reductive chemical release of N-glycans as 1-amino-alditols	0:58	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	13	42	theme	side	2464:2467	arg1	reactions					2469:2477	remarkable side reactions	2453:2477	remarkable side reactions	2453:2477	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	4	43	theme	ammonia	580:586	arg1	solution					588:595	aqueous ammonia solution	572:595	aqueous ammonia solution containing NaBH3CN as the reaction medium	572:637	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	13	44	dep	convenient	2489:2498	arg1	rapid					2501:2505	rapid	2501:2505	rapid	2501:2505	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	10	45	theme	biological	1860:1869	arg1	activities					1871:1880	various biological activities	1852:1880	various biological activities	1852:1880	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	0	46	theme	subsequent	64:73	arg1	labeling					104:111	subsequent 9-fluorenylmethyloxycarbonyl labeling	64:111	subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis	64:137	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	3	47	theme	glycans	484:490	arg1	dissociation					459:470	dissociation	459:470	dissociation of N-linked glycans from glycoproteins	459:509	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	0	48	theme	N-glycans	30:38	arg1	release					19:25	Reductive chemical release	0:25	Reductive chemical release of N-glycans as 1-amino-alditols	0:58	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	0	48	theme	N-glycans	30:38	arg1	labeling					104:111	subsequent 9-fluorenylmethyloxycarbonyl labeling	64:111	subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis	64:137	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	13	49	theme	functionalized	2424:2437	arg1	forms					2439:2443	functionalized forms	2424:2443	functionalized forms without remarkable side reactions	2424:2477	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	13	49	theme	functionalized	2424:2437	arg1	types					2402:2406	all types	2398:2406	all types of N-glycans	2398:2419	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	6	50	theme	peeling	917:923	arg1	degradation					925:935	peeling degradation	917:935	peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation	917:1006	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	3	51	theme	novel	429:433	arg1	method					435:440	a novel method	427:440	a novel method	427:440	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	4	52	theme	new	553:555	arg1	method					557:562	This new method	548:562	This new method	548:562	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	10	53	theme	N-Glycosylation	1681:1695	arg1	SIGNIFICANCE					1655:1666	SIGNIFICANCE	1655:1666	SIGNIFICANCE OF THE STUDY N-Glycosylation	1655:1695	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	8	54	with	compatible	1392:1401	arg1	fragmentation					1455:1467	sequential multi-stage mass spectrometry (MSn) fragmentation	1408:1467	sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing	1408:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	14	55	theme	N-glycan	2768:2775	arg1	targets					2785:2791	N-glycan related targets	2768:2791	N-glycan related targets of some drugs	2768:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	14	56	theme	targets	2785:2791	arg1	search					2711:2716	the search	2707:2716	the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs	2707:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	11	57	theme	glycan	2057:2062	arg1	epitopes					2064:2071	glycan epitopes	2057:2071	glycan epitopes	2057:2071	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	14	58	theme	diseases	2755:2762	arg1	biomarkers					2730:2739	N-glycan biomarkers	2721:2739	N-glycan biomarkers of some major diseases	2721:2762	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	14	58	theme	diseases	2755:2762	arg1	targets					2785:2791	N-glycan related targets	2768:2791	N-glycan related targets of some drugs	2768:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	12	59	theme	activities	2276:2285	arg1	pathologies					2295:2305	the pathologies	2291:2305	the pathologies of many diseases	2291:2322	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	12	59	theme	activities	2276:2285	arg1	mechanisms					2246:2255	the functional mechanisms	2231:2255	the functional mechanisms of many biological activities	2231:2285	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	2	60	theme	glycomics	403:411	arg1	lack					311:314	the lack	307:314	the lack of versatile methods to release N-glycans as functionalized forms	307:380	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	2	60	theme	glycomics	403:411	arg1	studies					413:419	undermining glycomics studies	391:419	undermining glycomics studies	391:419	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	13	61	theme	N-glycans	2411:2419	arg1	forms					2439:2443	functionalized forms	2424:2443	functionalized forms without remarkable side reactions	2424:2477	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	13	61	theme	N-glycans	2411:2419	arg1	types					2402:2406	all types	2398:2406	all types of N-glycans	2398:2419	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	12	62	theme	N-glycans	2190:2198	arg1	types					2181:2185	different types	2171:2185	different types of N-glycans	2171:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	13	63	from	samples	2586:2592	arg1	preparation					2520:2530	preparation	2520:2530	preparation of released N-glycans from various complex biological samples	2520:2592	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	13	63	from	samples	2586:2592	arg1	analysis					2507:2514	convenient, rapid analysis	2489:2514	convenient, rapid analysis	2489:2514	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	3	64	theme	online	534:539	arg1	LC/MS					541:545	online LC/MS	534:545	online LC/MS	534:545	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	12	65	theme	many	2260:2263	arg1	activities					2276:2285	many biological activities	2260:2285	many biological activities	2260:2285	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	6	66	theme	detectable	980:989	arg1	de-N-acetylation					991:1006	detectable de-N-acetylation	980:1006	detectable de-N-acetylation	980:1006	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	10	67	theme	modification	1750:1761	arg1	forms					1763:1767	the most common post-translational modification forms	1715:1767	the most common post-translational modification forms	1715:1767	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	5	68	theme	released	707:714	arg1	glycans					716:722	The released glycans	703:722	The released glycans	703:722	The released glycans are conveniently labeled with 9-fluorenylmethyloxycarbonyl (Fmoc) and analyzed by ESI-MS and online LC/MS.					
29885470	9	69	theme	methylated	1557:1566	arg1	carbamates					1568:1577	methylated carbamates	1557:1577	methylated carbamates for determination of branches and linkages	1557:1620	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	2	70	theme	release	340:346	arg1	N-glycans					348:356	release N-glycans	340:356	release N-glycans as functionalized forms	340:380	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	6	71	theme	structure	967:975	arg1	degradation					925:935	peeling degradation	917:935	peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation	917:1006	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	14	72	from	studies	2619:2625	arg1	relationship					2665:2676	the complicated structure-function relationship	2630:2676	the complicated structure-function relationship of N-glycans	2630:2689	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	4	73	theme	reaction	623:630	arg1	medium					632:637	the reaction medium	619:637	the reaction medium	619:637	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	4	73	theme	reaction	623:630	arg1	NaBH3CN					608:614	NaBH3CN	608:614	NaBH3CN	608:614	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	10	74	theme	common	1724:1729	arg1	modification					1750:1761	the most common post-translational modification	1715:1761	the most common post-translational modification forms	1715:1767	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	4	75	gly	glycoproteins	663:675	arg1	glycoproteins					663:675	glycoproteins	663:675	glycoproteins	663:675	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	13	76	theme	biological	2575:2584	arg1	samples					2586:2592	various complex biological samples	2559:2592	various complex biological samples	2559:2592	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	6	77	theme	core	944:947	arg1	structure					967:975	the core α-1,3-fucosylated structure	940:975	the core α-1,3-fucosylated structure	940:975	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	10	78	theme	pivotal	1779:1785	arg1	roles					1787:1791	pivotal roles	1779:1791	pivotal roles	1779:1791	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	4	79	contain	containing	597:606	arg2	NaBH3CN					608:614	NaBH3CN	608:614	NaBH3CN	608:614	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	4	79	contain	containing	597:606	arg1	solution					588:595	aqueous ammonia solution	572:595	aqueous ammonia solution containing NaBH3CN as the reaction medium	572:637	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	4	79	contain	containing	597:606	arg2	medium					632:637	the reaction medium	619:637	the reaction medium	619:637	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	1	80	theme	processes	199:207	arg1	series					178:183	a series	176:183	a series of biological processes	176:207	Glycoproteins play pivotal roles in a series of biological processes and their glycosylation patterns need to be structurally and functionally characterized.					
29885470	3	81	link	N-linked	475:482	arg1	glycans					484:490	N-linked glycans	475:490	N-linked glycans	475:490	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	2	82	theme	methods	329:335	arg1	lack					311:314	the lack	307:314	the lack of versatile methods to release N-glycans as functionalized forms	307:380	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	2	82	theme	methods	329:335	arg1	studies					413:419	undermining glycomics studies	391:419	undermining glycomics studies	391:419	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	13	83	theme	N-glycans	2544:2552	arg1	preparation					2520:2530	preparation	2520:2530	preparation of released N-glycans from various complex biological samples	2520:2592	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	13	83	theme	N-glycans	2544:2552	arg1	analysis					2507:2514	convenient, rapid analysis	2489:2514	convenient, rapid analysis	2489:2514	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	8	84	theme	ammonium	1313:1320	arg1	cations					1322:1328	quaternary ammonium cations	1302:1328	quaternary ammonium cations	1302:1328	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	6	85	theme	various	1048:1054	arg1	types					1056:1060	various types	1048:1060	various types of N-glycans	1048:1073	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	8	86	theme	MS	1369:1370	arg1	sensitivity					1372:1382	the MS sensitivity	1365:1382	the MS sensitivity	1365:1382	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	10	87	theme	structural	1800:1809	arg1	regulation					1826:1835	the structural and functional regulation	1796:1835	the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases	1796:1927	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	7	88	theme	Moench	1160:1165	arg1	Pollen					1167:1172	Fagopyrum esculentum Moench Pollen	1139:1172	Fagopyrum esculentum Moench Pollen	1139:1172	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	4	89	from	glycoproteins	663:675	arg1	forms					696:700	1-amino-alditol forms	680:700	1-amino-alditol forms	680:700	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	4	89	from	glycoproteins	663:675	arg1	glycans					650:656	glycans	650:656	glycans from glycoproteins	650:675	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	13	90	theme	various	2559:2565	arg1	samples					2586:2592	various complex biological samples	2559:2592	various complex biological samples	2559:2592	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	4	91	theme	1-amino-alditol	680:694	arg1	forms					696:700	1-amino-alditol forms	680:700	1-amino-alditol forms	680:700	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	4	91	theme	1-amino-alditol	680:694	arg1	glycans					650:656	glycans	650:656	glycans from glycoproteins	650:675	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	7	92	theme	Fagopyrum	1139:1147	arg1	Pollen					1167:1172	Fagopyrum esculentum Moench Pollen	1139:1172	Fagopyrum esculentum Moench Pollen	1139:1172	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	7	93	theme	chicken	1120:1126	arg1	ovalbumin					1128:1136	chicken ovalbumin	1120:1136	chicken ovalbumin	1120:1136	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	9	94	theme	MSn	1636:1638	arg1	fragmentation					1640:1652	sequential MSn fragmentation	1625:1652	sequential MSn fragmentation	1625:1652	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	8	95	theme	reducing	1337:1344	arg1	compatible					1392:1401	compatible	1392:1401	compatible	1392:1401	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	8	95	theme	reducing	1337:1344	arg1	end					1346:1348	the reducing end	1333:1348	the reducing end	1333:1348	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	6	96	gly	α-1,3-fucosylated	949:965	arg1	structure					967:975	the core α-1,3-fucosylated structure	940:975	the core α-1,3-fucosylated structure	940:975	Using the method, the neutral and acidic N-glycans were successfully released without peeling degradation of the core α-1,3-fucosylated structure or detectable de-N-acetylation, revealing its general applicability to various types of N-glycans.					
29885470	8	97	theme	sequential	1408:1417	arg1	fragmentation					1455:1467	sequential multi-stage mass spectrometry (MSn) fragmentation	1408:1467	sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing	1408:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	0	98	theme	9-fluorenylmethyloxycarbonyl	75:102	arg1	labeling					104:111	subsequent 9-fluorenylmethyloxycarbonyl labeling	64:111	subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis	64:137	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	7	99	theme	Fmoc-derivatized	1080:1095	arg1	N-glycans					1097:1105	The Fmoc-derivatized N-glycans	1076:1105	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS	1076:1180	The Fmoc-derivatized N-glycans derived from chicken ovalbumin, Fagopyrum esculentum Moench Pollen and FBS were successfully analyzed by online LC/MS to distinguish isomers.					
29885470	13	100	theme	convenient	2489:2498	arg1	analysis					2507:2514	convenient, rapid analysis	2489:2514	convenient, rapid analysis	2489:2514	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	8	101	theme	mass	1431:1434	arg1	spectrometry					1436:1447	multi-stage mass spectrometry	1419:1447	sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing	1408:1489	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	8	101	theme	mass	1431:1434	arg1	MSn					1450:1452	MSn	1450:1452	MSn	1450:1452	The 1-amino-alditols were also permethylated to form quaternary ammonium cations at the reducing end, which enhance the MS sensitivity and are compatible with sequential multi-stage mass spectrometry (MSn) fragmentation for glycan sequencing.					
29885470	12	102	theme	structural	2125:2134	arg1	characterization					2151:2166	Detailed structural and functional characterization	2116:2166	Detailed structural and functional characterization of different types of N-glycans	2116:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	11	103	theme	molecule	1957:1964	arg1	type					1935:1938	a type	1933:1938	a type of informational molecule	1933:1964	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	12	104	theme	functional	2140:2149	arg1	characterization					2151:2166	Detailed structural and functional characterization	2116:2166	Detailed structural and functional characterization of different types of N-glycans	2116:2198	Detailed structural and functional characterization of different types of N-glycans is essential for understanding the functional mechanisms of many biological activities and the pathologies of many diseases.					
29885470	1	105	theme	glycosylation	219:231	arg1	patterns					233:240	their glycosylation patterns	213:240	their glycosylation patterns	213:240	Glycoproteins play pivotal roles in a series of biological processes and their glycosylation patterns need to be structurally and functionally characterized.					
29885470	14	106	theme	drugs	2801:2805	arg1	biomarkers					2730:2739	N-glycan biomarkers	2721:2739	N-glycan biomarkers of some major diseases	2721:2762	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	14	106	theme	drugs	2801:2805	arg1	targets					2785:2791	N-glycan related targets	2768:2791	N-glycan related targets of some drugs	2768:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	3	107	theme	N-linked	475:482	arg1	glycans					484:490	N-linked glycans	475:490	N-linked glycans	475:490	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	0	108	theme	chemical	10:17	arg1	release					19:25	Reductive chemical release	0:25	Reductive chemical release of N-glycans as 1-amino-alditols	0:58	Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.					
29885470	14	109	theme	major	2749:2753	arg1	diseases					2755:2762	some major diseases	2744:2762	some major diseases	2744:2762	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	11	110	theme	protein	2097:2103	arg1	receptors					2105:2113	their corresponding protein receptors	2077:2113	their corresponding protein receptors	2077:2113	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	13	111	theme	remarkable	2453:2462	arg1	reactions					2469:2477	remarkable side reactions	2453:2477	remarkable side reactions	2453:2477	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	10	112	theme	various	1852:1858	arg1	activities					1871:1880	various biological activities	1852:1880	various biological activities	1852:1880	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	13	113	theme	released	2535:2542	arg1	N-glycans					2544:2552	released N-glycans	2535:2552	released N-glycans	2535:2552	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	3	114	gly	glycoproteins	497:509	arg1	glycoproteins					497:509	glycoproteins	497:509	glycoproteins	497:509	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	10	115	theme	proteins	1840:1847	arg1	regulation					1826:1835	the structural and functional regulation	1796:1835	the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases	1796:1927	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	14	116	theme	related	2777:2783	arg1	targets					2785:2791	N-glycan related targets	2768:2791	N-glycan related targets of some drugs	2768:2805	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	4	117	theme	aqueous	572:578	arg1	solution					588:595	aqueous ammonia solution	572:595	aqueous ammonia solution containing NaBH3CN as the reaction medium	572:637	This new method employs aqueous ammonia solution containing NaBH3CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms.					
29885470	10	118	theme	STUDY	1675:1679	arg1	N-Glycosylation					1681:1695	THE STUDY N-Glycosylation	1671:1695	THE STUDY N-Glycosylation	1671:1695	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	11	119	gly	glycoproteins	1984:1996	arg1	glycoproteins					1984:1996	glycoproteins	1984:1996	glycoproteins	1984:1996	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	3	120	from	glycoproteins	497:509	arg1	dissociation					459:470	dissociation	459:470	dissociation of N-linked glycans from glycoproteins	459:509	Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS.					
29885470	2	121	theme	functionalized	361:374	arg1	forms					376:380	functionalized forms	361:380	functionalized forms	361:380	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	9	122	theme	Fmoc-labeled	1496:1507	arg1	N-glycans					1509:1517	The Fmoc-labeled N-glycans	1492:1517	The Fmoc-labeled N-glycans	1492:1517	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	9	122	theme	Fmoc-labeled	1496:1507	arg1	permethylated					1532:1544	permethylated	1532:1544	permethylated	1532:1544	The Fmoc-labeled N-glycans were further permethylated to produce methylated carbamates for determination of branches and linkages by sequential MSn fragmentation.					
29885470	10	123	from	regulation	1826:1835	arg1	activities					1871:1880	various biological activities	1852:1880	various biological activities	1852:1880	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	14	124	theme	N-glycans	2681:2689	arg1	relationship					2665:2676	the complicated structure-function relationship	2630:2676	the complicated structure-function relationship of N-glycans	2630:2689	It is very valuable for studies on the complicated structure-function relationship of N-glycans, as well as for the search of N-glycan biomarkers of some major diseases and N-glycan related targets of some drugs.					
29885470	11	125	theme	molecular	2026:2034	arg1	interactions					2036:2047	the molecular interactions	2022:2047	the molecular interactions between glycan epitopes and their corresponding protein receptors	2022:2113	As a type of informational molecule, the N-glycans of glycoproteins participate directly in the molecular interactions between glycan epitopes and their corresponding protein receptors.					
29885470	13	126	theme	complex	2567:2573	arg1	samples					2586:2592	various complex biological samples	2559:2592	various complex biological samples	2559:2592	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	10	127	from	proteins	1840:1847	arg1	activities					1871:1880	various biological activities	1852:1880	various biological activities	1852:1880	SIGNIFICANCE OF THE STUDY N-Glycosylation represents one of the most common post-translational modification forms and plays pivotal roles in the structural and functional regulation of proteins in various biological activities, relating closely to human health and diseases.					
29885470	13	128	dep	simple	2344:2349	arg1	versatile					2352:2360	versatile	2352:2360	versatile	2352:2360	Here we describe a simple, versatile method to indistinguishably release all types of N-glycans as functionalized forms without remarkable side reactions, enabling convenient, rapid analysis and preparation of released N-glycans from various complex biological samples.					
29885470	2	129	theme	undermining	391:401	arg1	lack					311:314	the lack	307:314	the lack of versatile methods to release N-glycans as functionalized forms	307:380	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
29885470	2	129	theme	undermining	391:401	arg1	studies					413:419	undermining glycomics studies	391:419	undermining glycomics studies	391:419	However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies.					
31586542	3	0	theme	%	707:707	arg1	glycan					709:714	45% glycan	705:714	45% glycan	705:714	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	1	gly	glycosylated	524:535	arg1	core					553:556	a fully glycosylated SIVmac239-gp120 core	516:556	a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	516:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	5	2	theme	SIV	882:884	arg1	Env					886:888	SIV Env	882:888	the SIV Env protein surface	878:904	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	1	3	theme	SIV-envelope	150:161	arg1	trimers					169:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers are both extensively glycosylated, and antibodies identified to date have been unable to fully neutralize SIVmac239.					
31586542	3	4	from	structure	503:511	arg1	complex					561:567	complex	561:567	complex with rhesus CD4	561:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	5	theme	SIVmac239-gp120	537:551	arg1	core					553:556	a fully glycosylated SIVmac239-gp120 core	516:556	a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	516:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	6	from	core	553:556	arg1	complex					561:567	complex	561:567	complex with rhesus CD4	561:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	0	7	theme	Functional	88:97	arg1	Similarity					99:108	Functional Similarity	88:108	Functional Similarity of SIV and HIV Glycan Shields	88:138	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	3	8	theme	core	553:556	arg1	structure					503:511	The co-crystal structure	488:511	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	488:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	8	theme	core	553:556	arg1	fragment					609:616	the antigen-binding fragment	589:616	the antigen-binding fragment of ITS90.03 at 2.5-Å resolution	589:648	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	9	from	fragment	609:616	arg1	complex					561:567	complex	561:567	complex with rhesus CD4	561:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	5	10	used	occupied	952:959	arg2	hole					937:940	a glycan hole	928:940	a glycan hole	928:940	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	0	11	theme	SIV	113:115	arg1	Similarity					99:108	Functional Similarity	88:108	Functional Similarity of SIV and HIV Glycan Shields	88:138	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	6	12	from	shielding	1106:1114	arg1	coverage					1087:1094	coverage	1087:1094	coverage of spike	1087:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	2	13	theme	antibody	363:370	arg1	structure					325:333	structure	325:333	structure	325:333	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	2	13	theme	antibody	363:370	arg1	isolation					314:322	isolation	314:322	isolation	314:322	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	2	13	theme	antibody	363:370	arg1	interactions					347:358	glycan interactions	340:358	glycan interactions	340:358	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	3	14	theme	glycosylated	524:535	arg1	core					553:556	a fully glycosylated SIVmac239-gp120 core	516:556	a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	516:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	4	15	dep	aligned	777:783	arg1	each					769:772	each	769:772	each	769:772	SIV-gp120 core, rhesus CD4, and their complex could each be aligned structurally to their human counterparts.					
31586542	1	16	gly	glycosylated	198:209	arg1	trimers					169:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers are both extensively glycosylated, and antibodies identified to date have been unable to fully neutralize SIVmac239.					
31586542	4	17	theme	rhesus	733:738	arg1	CD4					740:742	rhesus CD4	733:742	rhesus CD4	733:742	SIV-gp120 core, rhesus CD4, and their complex could each be aligned structurally to their human counterparts.					
31586542	6	18	from	antibody	1121:1128	arg1	counterpart					1072:1082	its HIV counterpart	1064:1082	its HIV counterpart in coverage of spike	1064:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	18	from	antibody	1121:1128	arg1	modulation					1135:1144	modulation	1135:1144	modulation of receptor accessibility	1135:1170	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	18	from	antibody	1121:1128	arg1	shielding					1106:1114	shielding	1106:1114	shielding from antibody	1106:1128	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	19	theme	the SIV	1009:1015	arg1	glycan					1017:1022	the SIV glycan	1009:1022	the SIV glycan shield	1009:1029	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	3	20	with	complex	561:567	arg1	CD4					581:583	rhesus CD4	574:583	rhesus CD4	574:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	21	theme	rhesus	574:579	arg1	CD4					581:583	rhesus CD4	574:583	rhesus CD4	574:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	2	22	theme	glycan	340:345	arg1	interactions					347:358	glycan interactions	340:358	glycan interactions	340:358	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	0	23	theme	HIV	121:123	arg1	Shields					132:138	HIV Glycan Shields	121:138	HIV Glycan Shields	121:138	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	6	24	theme	accessibility	1158:1170	arg1	counterpart					1072:1082	its HIV counterpart	1064:1082	its HIV counterpart in coverage of spike	1064:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	24	theme	accessibility	1158:1170	arg1	modulation					1135:1144	modulation	1135:1144	modulation of receptor accessibility	1135:1170	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	24	theme	accessibility	1158:1170	arg1	shielding					1106:1114	shielding	1106:1114	shielding from antibody	1106:1128	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	2	25	dep	isolation	314:322	arg1	the					310:312	the	310:312	the	310:312	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	2	26	theme	monoclonal	384:393	arg1	antibody					395:402	a monoclonal antibody	382:402	a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate	382:474	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	2	26	theme	monoclonal	384:393	arg1	antibody					363:370	antibody ITS90.03	363:379	antibody ITS90.03	363:379	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	6	27	theme	spike	1099:1103	arg1	coverage					1087:1094	coverage	1087:1094	coverage of spike	1087:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	5	28	theme	Env	886:888	arg1	surface					898:904	the SIV Env protein surface	878:904	the SIV Env protein surface	878:904	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	0	29	theme	Antibody	30:37	arg1	Structure					14:22	Structure	14:22	Structure	14:22	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	0	29	theme	Antibody	30:37	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	0	30	theme	Shields	132:138	arg1	Similarity					99:108	Functional Similarity	88:108	Functional Similarity of SIV and HIV Glycan Shields	88:138	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	2	31	theme	neutralization-resistant	443:466	arg1	isolate					468:474	the highly neutralization-resistant isolate	432:474	the highly neutralization-resistant isolate	432:474	Here, we report the isolation, structure, and glycan interactions of antibody ITS90.03, a monoclonal antibody that completely neutralized the highly neutralization-resistant isolate, SIVmac239.					
31586542	0	32	theme	Glycan	125:130	arg1	Shields					132:138	HIV Glycan Shields	121:138	HIV Glycan Shields	121:138	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	3	33	theme	antigen-binding	593:607	arg1	fragment					609:616	the antigen-binding fragment	589:616	the antigen-binding fragment of ITS90.03 at 2.5-Å resolution	589:648	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	34	theme	co-crystal	492:501	arg1	structure					503:511	The co-crystal structure	488:511	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	488:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	35	theme	resolution	639:648	arg1	structure					503:511	The co-crystal structure	488:511	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	488:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	35	theme	resolution	639:648	arg1	fragment					609:616	the antigen-binding fragment	589:616	the antigen-binding fragment of ITS90.03 at 2.5-Å resolution	589:648	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	5	36	theme	strains	976:982	arg1	%					967:967	∼83%	964:967	∼83% of SIV strains	964:982	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	5	36	theme	strains	976:982	arg1	strains					976:982	SIV strains	972:982	SIV strains	972:982	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	5	37	theme	protein	890:896	arg1	surface					898:904	the SIV Env protein surface	878:904	the SIV Env protein surface	878:904	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	5	38	theme	glycan	987:992	arg1	N238					994:997	glycan N238	987:997	glycan N238	987:997	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	4	39	theme	human	807:811	arg1	counterparts					813:824	their human counterparts	801:824	their human counterparts	801:824	SIV-gp120 core, rhesus CD4, and their complex could each be aligned structurally to their human counterparts.					
31586542	6	40	theme	receptor	1149:1156	arg1	accessibility					1158:1170	receptor accessibility	1149:1170	receptor accessibility	1149:1170	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	5	41	theme	SIV	972:974	arg1	strains					976:982	SIV strains	972:982	SIV strains	972:982	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	6	42	theme	HIV	1068:1070	arg1	counterpart					1072:1082	its HIV counterpart	1064:1082	its HIV counterpart in coverage of spike	1064:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	3	43	from	complex	561:567	arg1	structure					503:511	The co-crystal structure	488:511	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4	488:583	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	3	43	from	complex	561:567	arg1	fragment					609:616	the antigen-binding fragment	589:616	the antigen-binding fragment of ITS90.03 at 2.5-Å resolution	589:648	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	6	44	dep	glycan	1017:1022	arg1	shield					1024:1029	shield	1024:1029	shield	1024:1029	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	6	45	from	counterpart	1072:1082	arg1	coverage					1087:1094	coverage	1087:1094	coverage of spike	1087:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
31586542	1	46	dep	glycosylated	198:209	arg1	both					181:184	both	181:184	both	181:184	HIV- and SIV-envelope (Env) trimers are both extensively glycosylated, and antibodies identified to date have been unable to fully neutralize SIVmac239.					
31586542	4	47	theme	SIV-gp120	717:725	arg1	core					727:730	SIV-gp120 core	717:730	SIV-gp120 core	717:730	SIV-gp120 core, rhesus CD4, and their complex could each be aligned structurally to their human counterparts.					
31586542	0	48	theme	Isolate	62:68	arg1	SIVmac239					70:78	Isolate SIVmac239	62:78	Isolate SIVmac239	62:78	Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields.					
31586542	5	49	theme	glycan	930:935	arg1	hole					937:940	a glycan hole	928:940	a glycan hole	928:940	The structure revealed that glycans masked most of the SIV Env protein surface, with ITS90 targeting a glycan hole, which is occupied in ∼83% of SIV strains by glycan N238.					
31586542	3	50	theme	45	705:706	arg1	%					707:707	%	707:707	%	707:707	The co-crystal structure of a fully glycosylated SIVmac239-gp120 core in complex with rhesus CD4 and the antigen-binding fragment of ITS90.03 at 2.5-Å resolution revealed that ITS90 recognized an epitope comprised of 45% glycan.					
31586542	1	51	theme	HIV-	141:144	arg1	trimers					169:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers	141:175	HIV- and SIV-envelope (Env) trimers are both extensively glycosylated, and antibodies identified to date have been unable to fully neutralize SIVmac239.					
31586542	6	52	from	modulation	1135:1144	arg1	coverage					1087:1094	coverage	1087:1094	coverage of spike	1087:1103	Overall, the SIV glycan shield appears to functionally resemble its HIV counterpart in coverage of spike, shielding from antibody, and modulation of receptor accessibility.					
30060662	4	0	theme	higher	744:749	arg1	level					762:766	higher structural level	744:766	higher structural level	744:766	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	2	1	theme	structure	373:381	arg1	perspective					383:393	quaternary structure perspective	362:393	quaternary structure perspective	362:393	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	4	2	theme	thermodynamic	841:853	arg1	stability					855:863	thermodynamic stability	841:863	thermodynamic stability	841:863	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	3	3	theme	protein	574:580	arg1	structure					582:590	the local protein structure	564:590	the local protein structure	564:590	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	5	4	theme	N-glycans	1072:1080	arg1	tools					1093:1097	general tools	1085:1097	general tools that can enhance protein stability	1085:1132	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	4	theme	N-glycans	1072:1080	arg1	design					1062:1067	the rational design	1049:1067	the rational design of N-glycans	1049:1080	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	4	5	from	glyco-linkers	624:636	arg1	form					653:656	the unusual form	641:656	the unusual form of glyco-bridge and glyco-hairpin	641:690	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	4	6	theme	higher	812:817	arg1	stability					827:835	kinetic stability	819:835	kinetic stability	819:835	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	5	7	theme	quaternary	995:1004	arg1	perspective					1016:1026	a quaternary structure perspective	993:1026	a quaternary structure perspective	993:1026	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	0	8	from	Levels	47:52	arg1	Design					0:5	Design	0:5	Design of Glyco-Linkers at Multiple Structural Levels	0:52	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.					
30060662	5	9	theme	rational	1053:1060	arg1	tools					1093:1097	general tools	1085:1097	general tools that can enhance protein stability	1085:1132	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	9	theme	rational	1053:1060	arg1	design					1062:1067	the rational design	1049:1067	the rational design of N-glycans	1049:1080	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	10	theme	unique	922:927	arg1	insight					929:935	unique insight	922:935	unique insight into the interactions between glycans and proteins from a quaternary structure perspective	922:1026	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	4	11	with	monomers	730:737	arg1	level					762:766	higher structural level	744:766	higher structural level	744:766	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	0	12	theme	Structural	36:45	arg1	Levels					47:52	Multiple Structural Levels	27:52	Multiple Structural Levels	27:52	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.					
30060662	3	13	theme	local	568:572	arg1	structure					582:590	the local protein structure	564:590	the local protein structure	564:590	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	5	14	theme	structure	1006:1014	arg1	perspective					1016:1026	a quaternary structure perspective	993:1026	a quaternary structure perspective	993:1026	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	3	15	theme	inherent	413:420	arg1	N-glycans					422:430	the two inherent N-glycans	405:430	the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris	405:478	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	3	16	theme	Pichia	464:469	arg1	pastoris					471:478	Pichia pastoris	464:478	Pichia pastoris	464:478	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	2	17	theme	full-length	308:318	arg1	N-glycans					320:328	full-length N-glycans	308:328	full-length N-glycans	308:328	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	3	18	theme	glyco-linkers	493:505	arg1	"					506:506	"glyco-linkers"	492:506	"glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure	492:590	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	0	19	theme	Glyco-Linkers	10:22	arg1	Design					0:5	Design	0:5	Design of Glyco-Linkers at Multiple Structural Levels	0:52	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.					
30060662	4	20	with	domains	718:724	arg1	level					762:766	higher structural level	744:766	higher structural level	744:766	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	1	21	theme	molecular	161:169	arg1	basis					171:175	the molecular basis	157:175	the molecular basis	157:175	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood.					
30060662	4	22	theme	glyco-bridge	661:672	arg1	form					653:656	the unusual form	641:656	the unusual form of glyco-bridge and glyco-hairpin	641:690	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	3	23	theme	β-glucuronidase	435:449	arg1	N-glycans					422:430	the two inherent N-glycans	405:430	the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris	405:478	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	4	24	theme	structural	751:760	arg1	level					762:766	higher structural level	744:766	higher structural level	744:766	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	2	25	theme	experimental	228:239	arg1	techniques					259:268	experimental and computational techniques	228:268	experimental and computational techniques	228:268	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	0	26	theme	Multiple	27:34	arg1	Levels					47:52	Multiple Structural Levels	27:52	Multiple Structural Levels	27:52	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.					
30060662	4	27	theme	inherent	874:881	arg1	N-glycans					883:891	the inherent N-glycans	870:891	the inherent N-glycans	870:891	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	4	28	theme	glyco-hairpin	678:690	arg1	form					653:656	the unusual form	641:656	the unusual form of glyco-bridge and glyco-hairpin	641:690	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	2	29	theme	quaternary	362:371	arg1	structure					373:381	quaternary structure	362:381	quaternary structure perspective	362:393	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	1	30	contain	has	101:103	arg2	roles					114:118	critical roles	105:118	critical roles	105:118	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood.					
30060662	1	30	contain	has	101:103	arg1	N-glycosylation					85:99	N-glycosylation	85:99	N-glycosylation	85:99	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood.					
30060662	5	31	theme	protein	1116:1122	arg1	stability					1124:1132	protein stability	1116:1132	protein stability	1116:1132	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	3	32	theme	proximal	528:535	arg1	motifs					537:542	spatially proximal motifs	518:542	spatially proximal motifs	518:542	We found the two inherent N-glycans of β-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure.					
30060662	1	33	theme	protein	134:140	arg1	stability					142:150	protein stability	134:150	protein stability	134:150	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood.					
30060662	1	34	theme	critical	105:112	arg1	roles					114:118	critical roles	105:118	critical roles	105:118	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood.					
30060662	4	35	theme	unusual	645:651	arg1	form					653:656	the unusual form	641:656	the unusual form of glyco-bridge and glyco-hairpin	641:690	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	4	36	theme	kinetic	819:825	arg1	stability					827:835	kinetic stability	819:835	kinetic stability	819:835	We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans.					
30060662	2	37	theme	protein	339:345	arg1	stability					347:355	protein stability	339:355	protein stability	339:355	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	0	38	theme	Protein	66:72	arg1	Stability					74:82	Protein Stability	66:82	Protein Stability	66:82	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.					
30060662	2	39	theme	computational	245:257	arg1	techniques					259:268	experimental and computational techniques	228:268	experimental and computational techniques	228:268	In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective.					
30060662	5	40	from	perspective	1016:1026	arg1	glycans					967:973	glycans	967:973	glycans	967:973	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	40	from	perspective	1016:1026	arg1	proteins					979:986	proteins	979:986	proteins	979:986	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	41	theme	general	1085:1091	arg1	tools					1093:1097	general tools	1085:1097	general tools that can enhance protein stability	1085:1132	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
30060662	5	41	theme	general	1085:1091	arg1	design					1062:1067	the rational design	1049:1067	the rational design of N-glycans	1049:1080	Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.					
29596681	4	0	theme	IgG1	644:647	arg1	antibodies					649:658	therapeutic IgG1 antibodies	632:658	therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions	632:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	10	1	theme	IgG	1474:1476	arg1	antibodies					1478:1487	human IgG antibodies	1468:1487	human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1468:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	8	2	theme	sialic	1320:1325	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	10	3	theme	distinct	1506:1513	arg1	glycoforms					1515:1524	distinct glycoforms	1506:1524	distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1506:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	2	4	theme	recombinant	289:299	arg1	therapeutics					301:312	recombinant therapeutics	289:312	recombinant therapeutics	289:312	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	8	5	theme	novel	1246:1250	arg1	design					1287:1292	a novel monoantennary homogeneous N-glycan design	1244:1292	a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping	1244:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	1	6	theme	recombinant	182:192	arg1	production					194:203	recombinant production	182:203	recombinant production of therapeutic glycoproteins with different glycan structures	182:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	8	7	theme	homogeneous	1266:1276	arg1	design					1287:1292	a novel monoantennary homogeneous N-glycan design	1244:1292	a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping	1244:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	6	8	theme	biantennary	1036:1046	arg1	N-glycans					1048:1056	rather homogenous biantennary N-glycans	1018:1056	rather homogenous biantennary N-glycans with and without galactose (G0F, G2F)	1018:1094	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	4	9	theme	conserved	693:701	arg1	N-glycan					703:710	the conserved N-glycan	689:710	the conserved N-glycan at Asn297	689:720	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	1	10	theme	glycoproteins	220:232	arg1	production					194:203	recombinant production	182:203	recombinant production of therapeutic glycoproteins with different glycan structures	182:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	8	11	theme	complete	1299:1306	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	4	12	theme	effector	768:775	arg1	functions					777:785	antibody effector functions	759:785	antibody effector functions	759:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	6	13	theme	precise	990:996	arg1	engineering					1003:1013	stable precise gene engineering	983:1013	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	1	14	theme	new	130:132	arg1	technologies					103:114	Precise gene editing technologies	82:114	Precise gene editing technologies	82:114	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	14	theme	new	130:132	arg1	opportunities					134:146	providing new opportunities	120:146	providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures	120:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	4	15	theme	dampening	738:746	arg1	effects					748:754	remarkable dampening effects	727:754	remarkable dampening effects on antibody effector functions	727:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	6	16	gly	glycoform	1146:1154	arg1	N-glycans					1048:1056	rather homogenous biantennary N-glycans	1018:1056	rather homogenous biantennary N-glycans with and without galactose (G0F, G2F)	1018:1094	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	0	17	theme	gene	68:71	arg1	editing					73:79	precise gene editing	60:79	precise gene editing	60:79	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
29596681	1	18	theme	engineer	158:165	arg1	cells					172:176	stably engineer host cells	151:176	stably engineer host cells	151:176	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	5	19	from	N-glycans	943:951	arg1	IgG1					962:965	human IgG1	956:965	human IgG1	956:965	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	10	20	theme	design	1563:1568	arg1	testing					1530:1536	testing	1530:1536	testing	1530:1536	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	10	20	theme	design	1563:1568	arg1	selection					1542:1550	selection	1542:1550	selection	1542:1550	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	2	21	theme	pharmacokinetic	414:428	arg1	properties					430:439	pharmacokinetic properties	414:439	pharmacokinetic properties as well as bioactivity	414:462	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	5	22	theme	precise	811:817	arg1	engineering					824:834	precise gene engineering	811:834	precise gene engineering	811:834	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	6	23	theme	monosialylated	1123:1136	arg1	glycoform					1146:1154	the α2,6-linked monosialylated (G2FS1) glycoform	1107:1154	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	0	24	from	IgG1	36:39	arg1	cells					48:52	CHO cells	44:52	CHO cells	44:52	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
29596681	0	25	theme	precise	60:66	arg1	editing					73:79	precise gene editing	60:79	precise gene editing	60:79	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
29596681	0	26	theme	Glycoengineering	0:15	arg1	options					24:30	Glycoengineering design options	0:30	Glycoengineering design options for IgG1 in CHO cells	0:52	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
29596681	1	27	theme	Precise	82:88	arg1	technologies					103:114	Precise gene editing technologies	82:114	Precise gene editing technologies	82:114	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	27	theme	Precise	82:88	arg1	opportunities					134:146	providing new opportunities	120:146	providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures	120:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	3	28	theme	therapeutic	490:500	arg1	glycoproteins					502:514	therapeutic glycoproteins	490:514	therapeutic glycoproteins	490:514	Structures of glycans on therapeutic glycoproteins are important for circulation, biodistribution and bioactivity.					
29596681	1	29	with	glycoproteins	220:232	arg1	structures					256:265	different glycan structures	239:265	different glycan structures	239:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	30	theme	editing	95:101	arg1	technologies					103:114	Precise gene editing technologies	82:114	Precise gene editing technologies	82:114	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	30	theme	editing	95:101	arg1	opportunities					134:146	providing new opportunities	120:146	providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures	120:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	10	31	theme	different	1574:1582	arg1	applications					1596:1607	different therapeutic applications	1574:1607	different therapeutic applications	1574:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	3	32	gly	glycoproteins	502:514	arg1	glycoproteins					502:514	therapeutic glycoproteins	490:514	therapeutic glycoproteins	490:514	Structures of glycans on therapeutic glycoproteins are important for circulation, biodistribution and bioactivity.					
29596681	3	33	theme	glycans	479:485	arg1	Structures					465:474	Structures	465:474	Structures of glycans on therapeutic glycoproteins	465:514	Structures of glycans on therapeutic glycoproteins are important for circulation, biodistribution and bioactivity.					
29596681	6	34	gly	monosialylated	1123:1136	arg1	glycoform					1146:1154	the α2,6-linked monosialylated (G2FS1) glycoform	1107:1154	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	6	34	gly	monosialylated	1123:1136	arg1	N-glycans					1048:1056	rather homogenous biantennary N-glycans	1018:1056	rather homogenous biantennary N-glycans with and without galactose (G0F, G2F)	1018:1094	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	10	35	theme	engineered	1421:1430	arg1	options					1439:1445	The stably engineered design options	1410:1445	The stably engineered design options	1410:1445	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	0	36	theme	CHO	44:46	arg1	cells					48:52	CHO cells	44:52	CHO cells	44:52	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
29596681	4	37	contain	have	722:725	arg1	α1,6Fucose					675:684	the core α1,6Fucose	666:684	the core α1,6Fucose on the conserved N-glycan at Asn297	666:720	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	4	37	contain	have	722:725	arg2	effects					748:754	remarkable dampening effects	727:754	remarkable dampening effects on antibody effector functions	727:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	1	38	theme	glycan	249:254	arg1	structures					256:265	different glycan structures	239:265	different glycan structures	239:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	6	39	theme	stable	983:988	arg1	engineering					1003:1013	stable precise gene engineering	983:1013	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	2	40	theme	products	372:379	arg1	consistency					357:367	consistency	357:367	consistency	357:367	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	2	40	theme	products	372:379	arg1	quality					345:351	quality	345:351	quality	345:351	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	4	41	from	effects	748:754	arg1	functions					777:785	antibody effector functions	759:785	antibody effector functions	759:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	10	42	with	antibodies	1478:1487	arg1	array					1497:1501	an array	1494:1501	an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1494:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	10	43	theme	antibodies	1478:1487	arg1	production					1454:1463	production	1454:1463	production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1454:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	4	44	theme	therapeutic	632:642	arg1	antibodies					649:658	therapeutic IgG1 antibodies	632:658	therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions	632:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	10	45	theme	human	1468:1472	arg1	antibodies					1478:1487	human IgG antibodies	1468:1487	human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1468:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	2	46	theme	therapeutics	301:312	arg1	focus					330:334	a focus	328:334	a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity	328:462	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	2	46	theme	therapeutics	301:312	arg1	glycosylation					272:284	The glycosylation	268:284	The glycosylation of recombinant therapeutics	268:312	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	4	47	from	Asn297	715:720	arg1	N-glycan					703:710	the conserved N-glycan	689:710	the conserved N-glycan at Asn297	689:720	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	1	48	theme	host	167:170	arg1	cells					172:176	stably engineer host cells	151:176	stably engineer host cells	151:176	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	7	49	theme	substantial	1185:1195	arg1	glycoforms					1210:1219	substantial disialylated glycoforms	1185:1219	substantial disialylated glycoforms	1185:1219	We were unable to introduce substantial disialylated glycoforms.					
29596681	8	50	theme	α2,6-linked	1308:1318	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	2	51	gly	glycosylation	272:284	arg1	therapeutics					301:312	recombinant therapeutics	289:312	recombinant therapeutics	289:312	The glycosylation of recombinant therapeutics has long been a focus for both quality and consistency of products and for optimizing and improving pharmacokinetic properties as well as bioactivity.					
29596681	5	52	theme	design	840:845	arg1	options					847:853	design options	840:853	design options for N-glycosylation	840:873	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	1	53	gly	glycoproteins	220:232	arg1	glycoproteins					220:232	therapeutic glycoproteins	208:232	therapeutic glycoproteins with different glycan structures	208:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	5	54	theme	possible	930:937	arg1	options					922:928	options	922:928	options possible for N-glycans on human IgG1	922:965	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	6	55	theme	N-glycans	1048:1056	arg1	engineering					1003:1013	stable precise gene engineering	983:1013	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	6	55	theme	N-glycans	1048:1056	arg1	glycoform					1146:1154	the α2,6-linked monosialylated (G2FS1) glycoform	1107:1154	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	8	56	theme	acid	1327:1330	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	8	57	theme	monoantennary	1252:1264	arg1	design					1287:1292	a novel monoantennary homogeneous N-glycan design	1244:1292	a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping	1244:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	6	58	theme	homogenous	1025:1034	arg1	N-glycans					1048:1056	rather homogenous biantennary N-glycans	1018:1056	rather homogenous biantennary N-glycans with and without galactose (G0F, G2F)	1018:1094	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	4	59	from	α1,6Fucose	675:684	arg1	N-glycan					703:710	the conserved N-glycan	689:710	the conserved N-glycan at Asn297	689:720	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	1	60	theme	therapeutic	208:218	arg1	glycoproteins					220:232	therapeutic glycoproteins	208:232	therapeutic glycoproteins with different glycan structures	208:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	8	61	theme	N-glycan	1278:1285	arg1	design					1287:1292	a novel monoantennary homogeneous N-glycan design	1244:1292	a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping	1244:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	6	62	theme	gene	998:1001	arg1	engineering					1003:1013	stable precise gene engineering	983:1013	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	4	63	theme	antibody	759:766	arg1	functions					777:785	antibody effector functions	759:785	antibody effector functions	759:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	1	64	theme	providing	120:128	arg1	technologies					103:114	Precise gene editing technologies	82:114	Precise gene editing technologies	82:114	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	64	theme	providing	120:128	arg1	opportunities					134:146	providing new opportunities	120:146	providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures	120:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	5	65	theme	gene	819:822	arg1	engineering					824:834	precise gene engineering	811:834	precise gene engineering	811:834	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	8	66	with	design	1287:1292	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	4	67	theme	remarkable	727:736	arg1	effects					748:754	remarkable dampening effects	727:754	remarkable dampening effects on antibody effector functions	727:785	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	5	68	theme	human	956:960	arg1	IgG1					962:965	human IgG1	956:965	human IgG1	956:965	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	10	69	theme	glycoforms	1515:1524	arg1	array					1497:1501	an array	1494:1501	an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications	1494:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	9	70	theme	core	1393:1396	arg1	α1,6Fucose					1398:1407	core α1,6Fucose	1393:1407	core α1,6Fucose	1393:1407	All N-glycoforms may be engineered with and without core α1,6Fucose.					
29596681	10	71	theme	optimal	1555:1561	arg1	design					1563:1568	optimal design	1555:1568	optimal design for different therapeutic applications	1555:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	5	72	theme	CHO	878:880	arg1	cells					882:886	CHO cells	878:886	CHO cells	878:886	We previously explored precise gene engineering and design options for N-glycosylation in CHO cells, and here we focus on engineering options possible for N-glycans on human IgG1.					
29596681	6	73	theme	α2,6-linked	1111:1121	arg1	glycoform					1146:1154	the α2,6-linked monosialylated (G2FS1) glycoform	1107:1154	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	1	74	theme	gene	90:93	arg1	technologies					103:114	Precise gene editing technologies	82:114	Precise gene editing technologies	82:114	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	1	74	theme	gene	90:93	arg1	opportunities					134:146	providing new opportunities	120:146	providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures	120:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	10	75	theme	therapeutic	1584:1594	arg1	applications					1596:1607	different therapeutic applications	1574:1607	different therapeutic applications	1574:1607	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	6	76	dep	monosialylated	1123:1136	arg1	G2FS1					1139:1143	G2FS1	1139:1143	G2FS1	1139:1143	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	7	77	theme	disialylated	1197:1208	arg1	glycoforms					1210:1219	substantial disialylated glycoforms	1185:1219	substantial disialylated glycoforms	1185:1219	We were unable to introduce substantial disialylated glycoforms.					
29596681	7	78	gly	disialylated	1197:1208	arg1	glycoforms					1210:1219	substantial disialylated glycoforms	1185:1219	substantial disialylated glycoforms	1185:1219	We were unable to introduce substantial disialylated glycoforms.					
29596681	10	79	theme	design	1432:1437	arg1	options					1439:1445	The stably engineered design options	1410:1445	The stably engineered design options	1410:1445	The stably engineered design options enable production of human IgG antibodies with an array of distinct glycoforms for testing and selection of optimal design for different therapeutic applications.					
29596681	8	80	link	α2,6-linked	1308:1318	arg1	capping					1332:1338	complete α2,6-linked sialic acid capping	1299:1338	complete α2,6-linked sialic acid capping	1299:1338	Instead we engineered a novel monoantennary homogeneous N-glycan design with complete α2,6-linked sialic acid capping.					
29596681	1	81	theme	different	239:247	arg1	structures					256:265	different glycan structures	239:265	different glycan structures	239:265	Precise gene editing technologies are providing new opportunities to stably engineer host cells for recombinant production of therapeutic glycoproteins with different glycan structures.					
29596681	4	82	theme	core	670:673	arg1	α1,6Fucose					675:684	the core α1,6Fucose	666:684	the core α1,6Fucose on the conserved N-glycan at Asn297	666:720	In particular, the latter has been demonstrated for therapeutic IgG1 antibodies where the core α1,6Fucose on the conserved N-glycan at Asn297 have remarkable dampening effects on antibody effector functions.					
29596681	6	83	link	α2,6-linked	1111:1121	arg1	glycoform					1146:1154	the α2,6-linked monosialylated (G2FS1) glycoform	1107:1154	stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform	983:1154	We demonstrate stable precise gene engineering of rather homogenous biantennary N-glycans with and without galactose (G0F, G2F) as well as the α2,6-linked monosialylated (G2FS1) glycoform.					
29596681	3	84	from	Structures	465:474	arg1	glycoproteins					502:514	therapeutic glycoproteins	490:514	therapeutic glycoproteins	490:514	Structures of glycans on therapeutic glycoproteins are important for circulation, biodistribution and bioactivity.					
29596681	0	85	theme	design	17:22	arg1	options					24:30	Glycoengineering design options	0:30	Glycoengineering design options for IgG1 in CHO cells	0:52	Glycoengineering design options for IgG1 in CHO cells using precise gene editing.					
30299005	3	0	theme	less	692:695	arg1	aggregation					697:707	less aggregation	692:707	less aggregation	692:707	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	6	1	theme	glycan	1345:1350	arg1	precursors					1352:1361	glycan precursors	1345:1361	glycan precursors	1345:1361	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	1	2	theme	drug	286:289	arg1	discovery					291:299	biotherapeutics drug discovery	270:299	biotherapeutics drug discovery	270:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	5	3	gly	glycoproteins	907:919	arg1	glycoproteins					907:919	therapeutic glycoproteins	895:919	therapeutic glycoproteins containing non-Fc N-linked glycans	895:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	8	4	theme	CHO	1759:1761	arg1	systems					1782:1788	stable CHO protein production systems	1752:1788	stable CHO protein production systems	1752:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	7	5	theme	sialylation	1511:1521	arg1	content					1523:1529	increased sialylation content	1501:1529	increased sialylation content of N-glycans	1501:1542	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	1	6	theme	rapid	190:194	arg1	expression					155:164	Large-scale transient expression	133:164	Large-scale transient expression in mammalian cells	133:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	1	6	theme	rapid	190:194	arg1	technology					215:224	a rapid protein production technology	188:224	a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery	188:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	7	7	theme	transient	1551:1559	arg1	expression					1572:1581	transient ExpiCHO-S™ expression	1551:1581	transient ExpiCHO-S™ expression	1551:1581	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	1	8	theme	production	204:213	arg1	expression					155:164	Large-scale transient expression	133:164	Large-scale transient expression in mammalian cells	133:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	1	8	theme	production	204:213	arg1	technology					215:224	a rapid protein production technology	188:224	a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery	188:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	7	9	theme	uridine	1486:1492	arg1	addition					1460:1467	medium addition	1453:1467	medium addition of galactose and uridine	1453:1492	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	7	10	theme	galactose	1472:1480	arg1	addition					1460:1467	medium addition	1453:1467	medium addition of galactose and uridine	1453:1492	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	5	11	theme	CHO	1120:1122	arg1	system					1135:1140	a stable CHO expression system	1111:1140	a stable CHO expression system	1111:1140	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	3	12	theme	protein	603:609	arg1	materials					611:619	protein materials	603:619	protein materials	603:619	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	1	13	theme	Large-scale	133:143	arg1	expression					155:164	Large-scale transient expression	133:164	Large-scale transient expression in mammalian cells	133:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	1	13	theme	Large-scale	133:143	arg1	technology					215:224	a rapid protein production technology	188:224	a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery	188:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	6	14	theme	target	1283:1288	arg1	genes					1290:1294	the target genes	1279:1294	the target genes	1279:1294	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	5	15	theme	transient	974:982	arg1	ExpiCHO-S™					984:993	transient ExpiCHO-S™	974:993	transient ExpiCHO-S™	974:993	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	2	16	theme	human	515:519	arg1	kidney					531:536	human embryonic kidney	515:536	human embryonic kidney (HEK) 293 cells	515:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	2	16	theme	human	515:519	arg1	HEK					539:541	HEK	539:541	HEK	539:541	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	0	17	from	sialylation	91:101	arg1	glycoproteins					118:130	therapeutic glycoproteins	106:130	therapeutic glycoproteins	106:130	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	7	18	theme	glycosyltransferases	1418:1437	arg1	co-transfection					1399:1413	co-transfection	1399:1413	co-transfection of glycosyltransferases combined with medium addition of galactose and uridine	1399:1492	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	1	19	theme	mammalian	169:177	arg1	cells					179:183	mammalian cells	169:183	mammalian cells	169:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	5	20	theme	non-Fc	932:937	arg1	glycans					948:954	non-Fc N-linked glycans	932:954	non-Fc N-linked glycans	932:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	5	21	contain	containing	921:930	arg2	glycans					948:954	non-Fc N-linked glycans	932:954	non-Fc N-linked glycans	932:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	5	21	contain	containing	921:930	arg1	glycoproteins					907:919	therapeutic glycoproteins	895:919	therapeutic glycoproteins containing non-Fc N-linked glycans	895:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	10	22	dep	2019	1869:1872	arg1	Prog.					1851:1855	Prog.	1851:1855	Prog.	1851:1855	Prog., 35: e2724, 2019.					
30299005	10	22	dep	2019	1869:1872	arg1	e2724					1862:1866	e2724	1862:1866	e2724	1862:1866	Prog., 35: e2724, 2019.					
30299005	0	23	theme	enhanced	73:80	arg1	sialylation					91:101	its enhanced N-glycan sialylation	69:101	its enhanced N-glycan sialylation	69:101	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	8	24	theme	scientific	1614:1623	arg1	basis					1625:1629	a scientific basis	1612:1629	a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1612:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	0	25	gly	glycoproteins	118:130	arg1	glycoproteins					118:130	therapeutic glycoproteins	106:130	therapeutic glycoproteins	106:130	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	7	26	theme	N-glycans	1534:1542	arg1	content					1523:1529	increased sialylation content	1501:1529	increased sialylation content of N-glycans	1501:1542	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	2	27	theme	kidney	531:536	arg1	cells					548:552	human embryonic kidney (HEK) 293 cells	515:552	human embryonic kidney (HEK) 293 cells	515:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	2	28	theme	cell	403:406	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	0	29	theme	therapeutic	106:116	arg1	glycoproteins					118:130	therapeutic glycoproteins	106:130	therapeutic glycoproteins	106:130	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	30	theme	expression	460:469	arg1	titers					471:476	high recombinant antibody expression titers	434:476	high recombinant antibody expression titers	434:476	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	4	31	theme	fluorescent	740:750	arg1	protein					752:758	Green fluorescent protein	734:758	Green fluorescent protein imaging data	734:771	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	2	32	theme	ExpiCHO-S™	391:400	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	0	33	theme	Transient	0:8	arg1	expression					14:23	Transient CHO expression	0:23	Transient CHO expression	0:23	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	34	theme	recombinant	439:449	arg1	titers					471:476	high recombinant antibody expression titers	434:476	high recombinant antibody expression titers	434:476	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	8	35	theme	transient	1655:1663	arg1	system					1669:1674	a future transient CHO system	1646:1674	a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1646:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	5	36	theme	glycan	1000:1005	arg1	pattern					1007:1013	the glycan pattern	996:1013	the glycan pattern	996:1013	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	3	37	theme	quality	661:667	arg1	characteristics					669:683	protein quality characteristics	653:683	protein quality characteristics (i.e., less aggregation)	653:708	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	4	38	theme	transient	833:841	arg1	expression					851:860	transient protein expression	833:860	transient protein expression	833:860	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	2	39	theme	capable	413:419	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	5	40	gly	sialylated	1050:1059	arg1	N-glycans					1061:1069	few sialylated N-glycans	1046:1069	few sialylated N-glycans	1046:1069	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	8	41	theme	N-glycan	1681:1688	arg1	compositions					1690:1701	N-glycan compositions	1681:1701	N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1681:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	2	42	theme	ovary	373:377	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	0	43	theme	antibody	45:52	arg1	production					54:63	robust antibody production	38:63	robust antibody production	38:63	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	44	theme	Chinese	357:363	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	8	45	theme	protein	1763:1769	arg1	systems					1782:1788	stable CHO protein production systems	1752:1788	stable CHO protein production systems	1752:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	6	46	theme	N-glycan	1154:1161	arg1	sialylation					1163:1173	N-glycan sialylation	1154:1173	N-glycan sialylation in transient ExpiCHO-S™	1154:1197	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	2	47	theme	host	385:388	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	4	48	dep	delayed	811:817	arg1	expression					851:860	transient protein expression	833:860	transient protein expression	833:860	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	5	49	theme	sialylated	1050:1059	arg1	N-glycans					1061:1069	few sialylated N-glycans	1046:1069	few sialylated N-glycans	1046:1069	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	2	50	theme	CHO	380:382	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	1	51	theme	biotherapeutics	270:284	arg1	discovery					291:299	biotherapeutics drug discovery	270:299	biotherapeutics drug discovery	270:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	5	52	contain	have	1041:1044	arg2	N-glycans					1061:1069	few sialylated N-glycans	1046:1069	few sialylated N-glycans	1046:1069	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	5	52	contain	have	1041:1044	arg1	pattern					1007:1013	the glycan pattern	996:1013	the glycan pattern	996:1013	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	7	53	theme	ExpiCHO-S™	1561:1570	arg1	expression					1572:1581	transient ExpiCHO-S™ expression	1551:1581	transient ExpiCHO-S™ expression	1551:1581	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	8	54	theme	stable	1752:1757	arg1	systems					1782:1788	stable CHO protein production systems	1752:1788	stable CHO protein production systems	1752:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	6	55	theme	sialyltransferase	1244:1260	arg1	genes					1262:1266	galactosyltransferase and sialyltransferase genes	1218:1266	genes	1262:1266	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	5	56	dep	glycans	1087:1093	arg1	contrast					1075:1082	contrast	1075:1082	contrast	1075:1082	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	6	57	theme	culture	1325:1331	arg1	medium					1333:1338	the culture medium	1321:1338	supplemented the culture medium	1308:1338	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	5	58	theme	therapeutic	895:905	arg1	glycoproteins					907:919	therapeutic glycoproteins	895:919	therapeutic glycoproteins containing non-Fc N-linked glycans	895:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	6	59	theme	galactosyltransferase	1218:1238	arg1	genes					1262:1266	galactosyltransferase and sialyltransferase genes	1218:1266	genes	1262:1266	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	6	60	theme	supplemented	1308:1319	arg1	medium					1333:1338	the culture medium	1321:1338	supplemented the culture medium	1308:1338	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	1	61	theme	protein	196:202	arg1	expression					155:164	Large-scale transient expression	133:164	Large-scale transient expression in mammalian cells	133:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	1	61	theme	protein	196:202	arg1	technology					215:224	a rapid protein production technology	188:224	a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery	188:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	7	62	theme	increased	1501:1509	arg1	content					1523:1529	increased sialylation content	1501:1529	increased sialylation content of N-glycans	1501:1542	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	2	63	theme	transient	331:339	arg1	expression					341:350	transient expression	331:350	transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	331:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	9	64	theme	Chemical	1820:1827	arg1	Engineers					1829:1837	Chemical Engineers	1820:1837	Chemical Engineers	1820:1837	© 2018 American Institute of Chemical Engineers Biotechnol.					
30299005	5	65	theme	stable	1113:1118	arg1	system					1135:1140	a stable CHO expression system	1111:1140	a stable CHO expression system	1111:1140	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	5	66	theme	expression	1124:1133	arg1	system					1135:1140	a stable CHO expression system	1111:1140	a stable CHO expression system	1111:1140	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	3	67	theme	ExpiCHO-S™	576:585	arg1	cells					587:591	ExpiCHO-S™ cells	576:591	ExpiCHO-S™ cells	576:591	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	6	68	dep	along	1268:1272	arg1	with					1274:1277	with	1274:1277	with	1274:1277	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	3	69	theme	better	626:631	arg1	titers					633:638	better titers	626:638	better titers	626:638	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	1	70	theme	transient	145:153	arg1	expression					155:164	Large-scale transient expression	133:164	Large-scale transient expression in mammalian cells	133:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	1	70	theme	transient	145:153	arg1	technology					215:224	a rapid protein production technology	188:224	a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery	188:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	7	71	theme	medium	1453:1458	arg1	addition					1460:1467	medium addition	1453:1467	medium addition of galactose and uridine	1453:1492	The authors have demonstrated that co-transfection of glycosyltransferases combined with medium addition of galactose and uridine led to increased sialylation content of N-glycans during transient ExpiCHO-S™ expression.					
30299005	0	72	theme	N-glycan	82:89	arg1	sialylation					91:101	its enhanced N-glycan sialylation	69:101	its enhanced N-glycan sialylation	69:101	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	5	73	theme	N-linked	939:946	arg1	glycans					948:954	non-Fc N-linked glycans	932:954	non-Fc N-linked glycans	932:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	2	74	from	expression	341:350	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	0	75	from	production	54:63	arg1	glycoproteins					118:130	therapeutic glycoproteins	106:130	therapeutic glycoproteins	106:130	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	76	theme	embryonic	521:529	arg1	kidney					531:536	human embryonic kidney	515:536	human embryonic kidney (HEK) 293 cells	515:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	2	76	theme	embryonic	521:529	arg1	HEK					539:541	HEK	539:541	HEK	539:541	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	8	77	theme	future	1648:1653	arg1	system					1669:1674	a future transient CHO system	1646:1674	a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1646:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	0	78	theme	CHO	10:12	arg1	expression					14:23	Transient CHO expression	0:23	Transient CHO expression	0:23	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	1	79	from	expression	155:164	arg1	cells					179:183	mammalian cells	169:183	mammalian cells	169:183	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	0	80	gly	sialylation	91:101	arg1	glycoproteins					118:130	therapeutic glycoproteins	106:130	therapeutic glycoproteins	106:130	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	81	theme	antibody	451:458	arg1	titers					471:476	high recombinant antibody expression titers	434:476	high recombinant antibody expression titers	434:476	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	8	82	theme	CHO	1665:1667	arg1	system					1669:1674	a future transient CHO system	1646:1674	a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1646:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	6	83	gly	sialylation	1163:1173	arg1	ExpiCHO-S™					1188:1197	transient ExpiCHO-S™	1178:1197	transient ExpiCHO-S™	1178:1197	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	4	84	theme	Green	734:738	arg1	protein					752:758	Green fluorescent protein	734:758	Green fluorescent protein imaging data	734:771	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	3	85	dep	aggregation	697:707	arg1	i.e.					686:689	i.e.	686:689	i.e.	686:689	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	5	86	link	N-linked	939:946	arg1	glycans					948:954	non-Fc N-linked glycans	932:954	non-Fc N-linked glycans	932:954	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	2	87	theme	high	434:437	arg1	titers					471:476	high recombinant antibody expression titers	434:476	high recombinant antibody expression titers	434:476	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	3	88	theme	protein	653:659	arg1	characteristics					669:683	protein quality characteristics	653:683	protein quality characteristics (i.e., less aggregation)	653:708	For some antibodies, ExpiCHO-S™ cells generated protein materials with better titers and improved protein quality characteristics (i.e., less aggregation) than those from HEK293.					
30299005	0	89	theme	robust	38:43	arg1	production					54:63	robust antibody production	38:63	robust antibody production	38:63	Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins.					
30299005	2	90	theme	comparable	479:488	arg1	titers					471:476	high recombinant antibody expression titers	434:476	high recombinant antibody expression titers	434:476	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	4	91	theme	prolonged	823:831	arg1	process					862:868	a delayed but prolonged transient protein expression process	809:868	a delayed but prolonged transient protein expression process	809:868	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	6	92	from	sialylation	1163:1173	arg1	ExpiCHO-S™					1188:1197	transient ExpiCHO-S™	1178:1197	transient ExpiCHO-S™	1178:1197	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	6	93	theme	transient	1178:1186	arg1	ExpiCHO-S™					1188:1197	transient ExpiCHO-S™	1178:1197	transient ExpiCHO-S™	1178:1197	To improve N-glycan sialylation in transient ExpiCHO-S™, we co-transfected galactosyltransferase and sialyltransferase genes along with the target genes, as well as supplemented the culture medium with glycan precursors.					
30299005	4	94	theme	protein	843:849	arg1	expression					851:860	transient protein expression	833:860	transient protein expression	833:860	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30299005	2	95	theme	hamster	365:371	arg1	line					408:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line	355:411	a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells	355:552	In this study we demonstrate transient expression in a Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody expression titers, comparable to levels obtained using human embryonic kidney (HEK) 293 cells.					
30299005	8	96	theme	production	1771:1780	arg1	systems					1782:1788	stable CHO protein production systems	1752:1788	stable CHO protein production systems	1752:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	8	97	with	system	1669:1674	arg1	compositions					1690:1701	N-glycan compositions	1681:1701	N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems	1681:1788	These results have provided a scientific basis for developing a future transient CHO system with N-glycan compositions that are similar to those profiles obtained from stable CHO protein production systems.					
30299005	1	98	theme	overall	248:254	arg1	timelines					256:264	overall timelines	248:264	overall timelines for biotherapeutics drug discovery	248:299	Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery.					
30299005	5	99	theme	few	1046:1048	arg1	N-glycans					1061:1069	few sialylated N-glycans	1046:1069	few sialylated N-glycans	1046:1069	When therapeutic glycoproteins containing non-Fc N-linked glycans were expressed in transient ExpiCHO-S™, the glycan pattern was unexpectedly found to have few sialylated N-glycans, in contrast to glycans produced within a stable CHO expression system.					
30299005	4	100	theme	delayed	811:817	arg1	process					862:868	a delayed but prolonged transient protein expression process	809:868	a delayed but prolonged transient protein expression process	809:868	Green fluorescent protein imaging data indicated that ExpiCHO-S™ displayed a delayed but prolonged transient protein expression process compared to HEK293.					
30484241	3	0	gly	glycoproteins	531:543	arg1	glycoproteins					531:543	synthetic glycoproteins	521:543	synthetic glycoproteins	521:543	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	3	1	theme	structural	555:564	arg1	analysis					566:573	structural analysis	555:573	structural analysis	555:573	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	0	2	from	Effects	0:6	arg1	Folding					37:43	Glycoprotein Folding	24:43	Glycoprotein Folding	24:43	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics.					
30484241	0	2	from	Effects	0:6	arg1	Dynamics					57:64	Protein Dynamics	49:64	Protein Dynamics	49:64	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics.					
30484241	1	3	theme	synthetic	105:113	arg1	glycosylpolypeptides					127:146	synthetic homogeneous glycosylpolypeptides	105:146	synthetic homogeneous glycosylpolypeptides	105:146	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	4	4	gly	glycoproteins	669:681	arg1	glycoproteins					669:681	glycoproteins	669:681	glycoproteins	669:681	Based on experimental results, it can be suggested that N-glycans stabilize the structure of glycoproteins.					
30484241	2	5	theme	protein	303:309	arg1	folding					311:317	protein folding	303:317	protein folding	303:317	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	1	6	gly	glycosylation	207:219	arg2	sites					221:225	glycosylation sites	207:225	glycosylation sites	207:225	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	3	7	theme	glycoproteins	531:543	arg1	15N-labeling					505:516	15N-labeling	505:516	15N-labeling of synthetic glycoproteins	505:543	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	2	8	theme	glycoprotein	341:352	arg1	models					354:359	small glycoprotein models	335:359	small glycoprotein models	335:359	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	2	9	theme	small	335:339	arg1	models					354:359	small glycoprotein models	335:359	small glycoprotein models	335:359	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	2	10	from	role	275:278	arg1	folding					311:317	protein folding	303:317	protein folding	303:317	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	1	11	theme	glycosylation	207:219	arg1	sites					221:225	glycosylation sites	207:225	glycosylation sites	207:225	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	0	12	theme	N-Glycans	11:19	arg1	Effects					0:6	Effects	0:6	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics	0:64	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics.					
30484241	1	13	theme	sites	221:225	arg1	number					197:202	number	197:202	number	197:202	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	1	13	theme	sites	221:225	arg1	position					184:191	position	184:191	position	184:191	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	2	14	theme	homogeneous	362:372	arg1	glycoproteins					384:396	homogeneous misfolded glycoproteins	362:396	homogeneous misfolded glycoproteins	362:396	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	2	15	gly	glycoproteins	384:396	arg1	glycoproteins					384:396	homogeneous misfolded glycoproteins	362:396	homogeneous misfolded glycoproteins	362:396	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	3	16	theme	unique	487:492	arg1	method					494:499	a unique method	485:499	a unique method for 15N-labeling of synthetic glycoproteins	485:543	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	0	17	theme	Glycoprotein	24:35	arg1	Folding					37:43	Glycoprotein Folding	24:43	Glycoprotein Folding	24:43	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics.					
30484241	1	18	theme	oligosaccharide	231:245	arg1	structures					247:256	oligosaccharide structures	231:256	oligosaccharide structures	231:256	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	0	19	theme	Protein	49:55	arg1	Dynamics					57:64	Protein Dynamics	49:64	Protein Dynamics	49:64	Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics.					
30484241	1	20	dep	position	184:191	arg1	the					180:182	the	180:182	the	180:182	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	2	21	theme	misfolded	374:382	arg1	glycoproteins					384:396	homogeneous misfolded glycoproteins	362:396	homogeneous misfolded glycoproteins	362:396	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	3	22	theme	chemical	441:448	arg1	syntheses					450:458	these chemical syntheses	435:458	these chemical syntheses	435:458	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	1	23	gly	glycoproteins	153:165	arg1	glycoproteins					153:165	glycoproteins	153:165	glycoproteins	153:165	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	1	24	theme	homogeneous	115:125	arg1	glycosylpolypeptides					127:146	synthetic homogeneous glycosylpolypeptides	105:146	synthetic homogeneous glycosylpolypeptides	105:146	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	4	25	theme	experimental	585:596	arg1	results					598:604	experimental results	585:604	experimental results	585:604	Based on experimental results, it can be suggested that N-glycans stabilize the structure of glycoproteins.					
30484241	2	26	theme	oligosaccharides	283:298	arg1	role					275:278	the role	271:278	the role of oligosaccharides in protein folding	271:317	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
30484241	1	27	theme	glycosylpolypeptides	127:146	arg1	folding					94:100	the folding	90:100	the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins	90:165	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	1	28	theme	structures	247:256	arg1	number					197:202	number	197:202	number	197:202	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	1	28	theme	structures	247:256	arg1	position					184:191	position	184:191	position	184:191	This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures.					
30484241	3	29	theme	synthetic	521:529	arg1	glycoproteins					531:543	synthetic glycoproteins	521:543	synthetic glycoproteins	521:543	In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis.					
30484241	4	30	theme	glycoproteins	669:681	arg1	structure					656:664	the structure	652:664	the structure of glycoproteins	652:681	Based on experimental results, it can be suggested that N-glycans stabilize the structure of glycoproteins.					
30484241	2	31	gly	glycoprotein	341:352	arg1	glycoprotein					341:352	small glycoprotein models	335:359	small glycoprotein models	335:359	To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins.					
29479898	12	0	theme	LEL	1720:1722	arg1	results					1709:1715	lectin blot results	1697:1715	lectin blot results of LEL, STL, PHA-L, RCA-I	1697:1741	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	4	1	theme	samples	560:566	arg1	profiles					542:549	the glycan profiles	531:549	the glycan profiles of serum samples between early gastric cancer and healthy controls	531:616	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	2	2	theme	advanced	338:345	arg1	stage					347:351	an advanced stage	335:351	an advanced stage	335:351	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	4	3	dep	METHODS	443:449	arg1	microarray					460:469	A lectin microarray	451:469	METHODS A lectin microarray with 50 kinds of tumor-associated lectin	443:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	4	4	theme	early	576:580	arg1	cancer					590:595	early gastric cancer	576:595	early gastric cancer	576:595	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	12	5	theme	lectin	1697:1702	arg1	results					1709:1715	lectin blot results	1697:1715	lectin blot results of LEL, STL, PHA-L, RCA-I	1697:1741	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	11	6	theme	recognized	1569:1578	arg1	structure					1605:1613	their specifically recognized N-acetyl-D-galactosamine structure	1550:1613	their specifically recognized N-acetyl-D-galactosamine structure	1550:1613	In addition, their specifically recognized N-acetyl-D-galactosamine structure and (α-1,3) mannose residues were decreased in early gastric cancer.					
29479898	7	7	theme	early	1086:1090	arg1	cancer					1100:1105	early gastric cancer	1086:1105	early gastric cancer	1086:1105	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	13	8	theme	study	1834:1838	arg1	findings					1818:1825	The findings	1814:1825	The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer	1814:1931	CONCLUSIONS The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer.					
29479898	4	9	used	used	516:519	arg2	METHODS					443:449	METHODS	443:449	METHODS A lectin microarray with 50 kinds of tumor-associated lectin	443:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	9	10	theme	6GlcNAc	1373:1379	arg1	structure					1391:1399	6GlcNAc branching structure	1373:1399	6GlcNAc branching structure	1373:1399	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	2	11	theme	most	291:294	arg1	cancers					304:310	most gastric cancers	291:310	most gastric cancers	291:310	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	1	12	theme	cancer	206:211	arg1	development					191:201	development	191:201	development	191:201	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	1	12	theme	cancer	206:211	arg1	occurrence					176:185	occurrence	176:185	occurrence	176:185	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	12	13	with	consistent	1748:1757	arg1	results					1768:1774	the results	1764:1774	the results of the lectin microarray	1764:1799	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	9	14	theme	α-linked	1402:1409	arg1	residues					1418:1425	α-linked fucose residues	1402:1425	α-linked fucose residues	1402:1425	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	2	15	with	cancer	241:246	arg1	mortality					272:280	mortality	272:280	mortality	272:280	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	2	15	with	cancer	241:246	arg1	morbidity					258:266	morbidity	258:266	morbidity	258:266	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	1	16	dep	BACKGROUND	75:84	arg1	reported					109:116	reported	109:116	have reported that protein glycosylation plays an important role in the occurrence and development of cancer	104:211	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	10	17	theme	binding	1520:1526	arg1	ability					1528:1534	their binding ability	1514:1534	their binding ability	1514:1534	While the two lectins CFL GNL reduced their binding ability.					
29479898	7	18	theme	recognized	1024:1033	arg1	GlcNAc					1035:1040	their specifically recognized GlcNAc	1005:1040	their specifically recognized GlcNAc	1005:1040	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	1	19	theme	protein	123:129	arg1	glycosylation					131:143	protein glycosylation	123:143	protein glycosylation	123:143	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	8	20	theme	early	1223:1227	arg1	cancer					1237:1242	the early gastric cancer	1219:1242	the early gastric cancer	1219:1242	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	2	21	theme	high	253:256	arg1	morbidity					258:266	morbidity	258:266	morbidity	258:266	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	11	22	theme	early	1662:1666	arg1	cancer					1676:1681	early gastric cancer	1662:1681	early gastric cancer	1662:1681	In addition, their specifically recognized N-acetyl-D-galactosamine structure and (α-1,3) mannose residues were decreased in early gastric cancer.					
29479898	1	23	theme	important	154:162	arg1	role					164:167	an important role	151:167	an important role	151:167	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	9	24	link	α-linked	1402:1409	arg1	residues					1418:1425	α-linked fucose residues	1402:1425	α-linked fucose residues	1402:1425	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	13	25	dep	study	1834:1838	arg1	clarify					1840:1846	clarify	1840:1846	clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer	1840:1931	CONCLUSIONS The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer.					
29479898	4	26	theme	tumor-associated	488:503	arg1	lectin					505:510	tumor-associated lectin	488:510	tumor-associated lectin	488:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	9	27	theme	gastric	1460:1466	arg1	cancer					1468:1473	gastric cancer	1460:1473	gastric cancer	1460:1473	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	4	28	theme	lectin	453:458	arg1	microarray					460:469	A lectin microarray	451:469	METHODS A lectin microarray with 50 kinds of tumor-associated lectin	443:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	14	29	theme	diagnostic	2012:2021	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	29	theme	diagnostic	2012:2021	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	12	30	theme	lectin	1783:1788	arg1	microarray					1790:1799	the lectin microarray	1779:1799	the lectin microarray	1779:1799	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	7	31	theme	lectins	929:935	arg1	intensities					910:920	the normalized fluorescent intensities	883:920	the normalized fluorescent intensities of the lectins PWA, LEL, and STL	883:953	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	14	32	theme	potential	1996:2004	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	32	theme	potential	1996:2004	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	6	33	dep	RESULTS	679:685	arg1	result					691:696	The result	687:696	RESULTS The result of the lectin microarray	679:721	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	3	34	theme	glycanbased	400:410	arg1	biomarkers					412:421	novel specific serum glycanbased biomarkers	379:421	novel specific serum glycanbased biomarkers for gastric cancer	379:440	Here, we aim to discover novel specific serum glycanbased biomarkers for gastric cancer.					
29479898	7	35	from	expression	1072:1081	arg1	cancer					1100:1105	early gastric cancer	1086:1105	early gastric cancer	1086:1105	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	0	36	theme	Early	18:22	arg1	Cancer					32:37	Early Gastric Cancer	18:37	Early Gastric Cancer	18:37	Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology.					
29479898	3	37	theme	specific	385:392	arg1	biomarkers					412:421	novel specific serum glycanbased biomarkers	379:421	novel specific serum glycanbased biomarkers for gastric cancer	379:440	Here, we aim to discover novel specific serum glycanbased biomarkers for gastric cancer.					
29479898	11	38	theme	mannose	1627:1633	arg1	residues					1635:1642	(α-1,3) mannose residues	1619:1642	(α-1,3) mannose residues	1619:1642	In addition, their specifically recognized N-acetyl-D-galactosamine structure and (α-1,3) mannose residues were decreased in early gastric cancer.					
29479898	0	39	theme	Cancer	32:37	arg1	Glycoprofiling					0:13	Glycoprofiling	0:13	Glycoprofiling of Early Gastric Cancer	0:37	Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology.					
29479898	9	40	theme	glycan	1276:1281	arg1	structures					1283:1292	These glycan structures	1270:1292	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen	1270:1441	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	14	41	theme	GlcNAc	1966:1971	arg1	structure					1973:1981	GlcNAc structure	1966:1981	GlcNAc structure	1966:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	6	42	theme	signal	739:744	arg1	intensities					746:756	the signal intensities	735:756	the signal intensities of 13 lectins	735:770	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	0	43	theme	Lectin	45:50	arg1	Technology					63:72	Lectin Microarray Technology	45:72	Lectin Microarray Technology	45:72	Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology.					
29479898	6	44	theme	early	836:840	arg1	cancer					850:855	early gastric cancer	836:855	early gastric cancer	836:855	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	4	45	theme	gastric	582:588	arg1	cancer					590:595	early gastric cancer	576:595	early gastric cancer	576:595	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	9	46	contain	containing	1294:1303	arg2	N-glycan					1358:1365	N-glycan	1358:1365	N-glycan	1358:1365	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	structure					1391:1399	6GlcNAc branching structure	1373:1399	6GlcNAc branching structure	1373:1399	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg1	structures					1283:1292	These glycan structures	1270:1292	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen	1270:1441	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	antigen					1435:1441	Tn antigen	1432:1441	Tn antigen	1432:1441	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	β-1					1368:1370	β-1	1368:1370	β-1	1368:1370	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	GalNAc					1305:1310	GalNAc	1305:1310	GalNAc	1305:1310	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	GlcNAc					1331:1336	terminal Galβ 1-4 GlcNAc	1313:1336	terminal Galβ 1-4 GlcNAc	1313:1336	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	46	contain	containing	1294:1303	arg2	residues					1418:1425	α-linked fucose residues	1402:1425	α-linked fucose residues	1402:1425	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	7	47	theme	normalized	887:896	arg1	intensities					910:920	the normalized fluorescent intensities	883:920	the normalized fluorescent intensities of the lectins PWA, LEL, and STL	883:953	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	9	48	theme	Galβ	1322:1325	arg1	GlcNAc					1331:1336	terminal Galβ 1-4 GlcNAc	1313:1336	terminal Galβ 1-4 GlcNAc	1313:1336	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	13	49	theme	gastric	1918:1924	arg1	cancer					1926:1931	gastric cancer	1918:1931	gastric cancer	1918:1931	CONCLUSIONS The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer.					
29479898	4	50	with	microarray	460:469	arg1	kinds					479:483	50 kinds	476:483	50 kinds of tumor-associated lectin	476:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	8	51	theme	binding	1122:1128	arg1	higher					1209:1214	higher	1209:1214	higher	1209:1214	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	51	theme	binding	1122:1128	arg1	affinity					1130:1137	the binding affinity	1118:1137	the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL	1118:1202	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	4	52	theme	serum	554:558	arg1	samples					560:566	serum samples	554:566	serum samples	554:566	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	7	53	theme	gastric	1092:1098	arg1	cancer					1100:1105	early gastric cancer	1086:1105	early gastric cancer	1086:1105	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	12	54	theme	blot	1704:1707	arg1	results					1709:1715	lectin blot results	1697:1715	lectin blot results of LEL, STL, PHA-L, RCA-I	1697:1741	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	6	55	theme	significant	779:789	arg1	differences					791:801	significant differences	779:801	significant differences between the healthy controls and early gastric cancer	779:855	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	8	56	theme	lectins	1146:1152	arg1	higher					1209:1214	higher	1209:1214	higher	1209:1214	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	56	theme	lectins	1146:1152	arg1	affinity					1130:1137	the binding affinity	1118:1137	the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL	1118:1202	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	11	57	theme	N-acetyl-D-galactosamine	1580:1603	arg1	structure					1605:1613	their specifically recognized N-acetyl-D-galactosamine structure	1550:1613	their specifically recognized N-acetyl-D-galactosamine structure	1550:1613	In addition, their specifically recognized N-acetyl-D-galactosamine structure and (α-1,3) mannose residues were decreased in early gastric cancer.					
29479898	7	58	theme	elevated	1063:1070	arg1	expression					1072:1081	an especially elevated expression	1049:1081	an especially elevated expression in early gastric cancer	1049:1105	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	6	59	theme	lectins	764:770	arg1	intensities					746:756	the signal intensities	735:756	the signal intensities of 13 lectins	735:770	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	5	60	theme	lectin	624:629	arg1	blot					631:634	lectin blot	624:634	lectin blot	624:634	Then lectin blot was performed to validate the differences.					
29479898	14	61	theme	gastric	2034:2040	arg1	cancer					2042:2047	gastric cancer	2034:2047	gastric cancer	2034:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	13	62	theme	specific	1852:1859	arg1	alterations					1861:1871	the specific alterations	1848:1871	the specific alterations for glycosylation	1848:1889	CONCLUSIONS The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer.					
29479898	2	63	theme	gastric	296:302	arg1	cancers					304:310	most gastric cancers	291:310	most gastric cancers	291:310	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	9	64	theme	branching	1381:1389	arg1	structure					1391:1399	6GlcNAc branching structure	1373:1399	6GlcNAc branching structure	1373:1399	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	2	65	theme	common	234:239	arg1	cancer					222:227	Gastric cancer	214:227	Gastric cancer	214:227	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	2	65	theme	common	234:239	arg1	cancer					241:246	a common cancer	232:246	a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage	232:351	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	9	66	theme	fucose	1411:1416	arg1	residues					1418:1425	α-linked fucose residues	1402:1425	α-linked fucose residues	1402:1425	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	9	67	theme	Tn	1432:1433	arg1	antigen					1435:1441	Tn antigen	1432:1441	Tn antigen	1432:1441	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	8	68	theme	gastric	1229:1235	arg1	cancer					1237:1242	the early gastric cancer	1219:1242	the early gastric cancer	1219:1242	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	11	69	theme	gastric	1668:1674	arg1	cancer					1676:1681	early gastric cancer	1662:1681	early gastric cancer	1662:1681	In addition, their specifically recognized N-acetyl-D-galactosamine structure and (α-1,3) mannose residues were decreased in early gastric cancer.					
29479898	4	70	theme	lectin	505:510	arg1	kinds					479:483	50 kinds	476:483	50 kinds of tumor-associated lectin	476:510	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	RCA-I					1167:1171	RCA-I	1167:1171	RCA-I	1167:1171	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	PHA-L					1184:1188	PHA-L	1184:1188	PHA-L	1184:1188	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	UEA					1191:1193	UEA	1191:1193	UEA	1191:1193	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	lectins					1146:1152	the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL	1142:1202	the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL	1142:1202	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	EEL					1154:1156	EEL	1154:1156	EEL	1154:1156	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	VAL					1174:1176	VAL	1174:1176	VAL	1174:1176	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	DSA					1179:1181	DSA	1179:1181	DSA	1179:1181	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	CAL					1200:1202	CAL	1200:1202	CAL	1200:1202	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	8	71	dep	lectins	1146:1152	arg1	RCA-II					1159:1164	RCA-II	1159:1164	RCA-II	1159:1164	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	7	72	dep	lectins	929:935	arg1	lectins					929:935	the lectins PWA, LEL, and STL	925:953	the lectins PWA, LEL, and STL	925:953	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	7	72	dep	lectins	929:935	arg1	LEL					942:944	LEL	942:944	LEL	942:944	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	7	72	dep	lectins	929:935	arg1	STL					951:953	STL	951:953	STL	951:953	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	7	72	dep	lectins	929:935	arg1	PWA					937:939	PWA	937:939	PWA	937:939	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	14	73	theme	specific	1938:1945	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	73	theme	specific	1938:1945	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	8	74	theme	healthy	1252:1258	arg1	controls					1260:1267	healthy controls	1252:1267	healthy controls	1252:1267	Moreover, the binding affinity of the lectins EEL, RCA-II, RCA-I, VAL, DSA, PHA-L, UEA, and CAL were higher in the early gastric cancer than in healthy controls.					
29479898	12	75	theme	microarray	1790:1799	arg1	results					1768:1774	the results	1764:1774	the results of the lectin microarray	1764:1799	Furthermore, lectin blot results of LEL, STL, PHA-L, RCA-I were consistent with the results of the lectin microarray.					
29479898	14	76	theme	early	2006:2010	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	76	theme	early	2006:2010	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	3	77	theme	gastric	427:433	arg1	cancer					435:440	gastric cancer	427:440	gastric cancer	427:440	Here, we aim to discover novel specific serum glycanbased biomarkers for gastric cancer.					
29479898	4	78	theme	glycan	535:540	arg1	profiles					542:549	the glycan profiles	531:549	the glycan profiles of serum samples between early gastric cancer and healthy controls	531:616	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	0	79	theme	Gastric	24:30	arg1	Cancer					32:37	Early Gastric Cancer	18:37	Early Gastric Cancer	18:37	Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology.					
29479898	6	80	theme	microarray	712:721	arg1	result					691:696	The result	687:696	RESULTS The result of the lectin microarray	679:721	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	3	81	theme	serum	394:398	arg1	biomarkers					412:421	novel specific serum glycanbased biomarkers	379:421	novel specific serum glycanbased biomarkers for gastric cancer	379:440	Here, we aim to discover novel specific serum glycanbased biomarkers for gastric cancer.					
29479898	14	82	theme	structure	1973:1981	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	82	theme	structure	1973:1981	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	13	83	theme	cancer	1926:1931	arg1	pathogenesis					1902:1913	the pathogenesis	1898:1913	the pathogenesis of gastric cancer	1898:1931	CONCLUSIONS The findings of our study clarify the specific alterations for glycosylation during the pathogenesis of gastric cancer.					
29479898	0	84	theme	Microarray	52:61	arg1	Technology					63:72	Lectin Microarray Technology	45:72	Lectin Microarray Technology	45:72	Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology.					
29479898	6	85	theme	gastric	842:848	arg1	cancer					850:855	early gastric cancer	836:855	early gastric cancer	836:855	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	3	86	theme	novel	379:383	arg1	biomarkers					412:421	novel specific serum glycanbased biomarkers	379:421	novel specific serum glycanbased biomarkers for gastric cancer	379:440	Here, we aim to discover novel specific serum glycanbased biomarkers for gastric cancer.					
29479898	2	87	theme	Gastric	214:220	arg1	cancer					222:227	Gastric cancer	214:227	Gastric cancer	214:227	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	2	87	theme	Gastric	214:220	arg1	cancer					241:246	a common cancer	232:246	a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage	232:351	Gastric cancer is a common cancer with high morbidity and mortality owing to most gastric cancers are discovered only at an advanced stage.					
29479898	1	88	dep	occurrence	176:185	arg1	the					172:174	the	172:174	the	172:174	BACKGROUND Recently, studies have reported that protein glycosylation plays an important role in the occurrence and development of cancer.					
29479898	7	89	theme	fluorescent	898:908	arg1	intensities					910:920	the normalized fluorescent intensities	883:920	the normalized fluorescent intensities of the lectins PWA, LEL, and STL	883:953	Compared to the healthy, the normalized fluorescent intensities of the lectins PWA, LEL, and STL were significantly increased, and it implied that their specifically recognized GlcNAc showed an especially elevated expression in early gastric cancer.					
29479898	14	90	theme	high	1947:1950	arg1	expression					1952:1961	The specific high expression	1934:1961	The specific high expression of GlcNAc structure	1934:1981	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	14	90	theme	high	1947:1950	arg1	marker					2023:2028	a potential early diagnostic marker	1994:2028	a potential early diagnostic marker for gastric cancer	1994:2047	The specific high expression of GlcNAc structure may act as a potential early diagnostic marker for gastric cancer.					
29479898	9	91	theme	terminal	1313:1320	arg1	GlcNAc					1331:1336	terminal Galβ 1-4 GlcNAc	1313:1336	terminal Galβ 1-4 GlcNAc	1313:1336	These glycan structures containing GalNAc, terminal Galβ 1-4 GlcNAc, Tri/tetraantennary N-glycan, β-1, 6GlcNAc branching structure, α-linked fucose residues, and Tn antigen were elevated in gastric cancer.					
29479898	6	92	theme	healthy	815:821	arg1	controls					823:830	the healthy controls	811:830	the healthy controls	811:830	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
29479898	4	93	theme	healthy	601:607	arg1	controls					609:616	healthy controls	601:616	healthy controls	601:616	METHODS A lectin microarray with 50 kinds of tumor-associated lectin was used to detect the glycan profiles of serum samples between early gastric cancer and healthy controls.					
29479898	6	94	theme	lectin	705:710	arg1	microarray					712:721	the lectin microarray	701:721	the lectin microarray	701:721	RESULTS The result of the lectin microarray showed that the signal intensities of 13 lectins showed significant differences between the healthy controls and early gastric cancer.					
31102532	8	0	dep	oncogenes	1337:1345	arg1	oncogenes					1337:1345	the oncogenes MYC and MYCN	1333:1358	the oncogenes MYC and MYCN	1333:1358	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	8	0	dep	oncogenes	1337:1345	arg1	MYCN					1355:1358	MYCN	1355:1358	MYCN	1355:1358	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	8	0	dep	oncogenes	1337:1345	arg1	MYC					1347:1349	MYC	1347:1349	MYC	1347:1349	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	8	1	theme	glycan	1426:1431	arg1	structures					1433:1442	the glycan structures	1422:1442	the glycan structures	1422:1442	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	3	2	theme	proteins	510:517	arg1	production					484:493	the production	480:493	the production of therapeutic proteins	480:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	7	3	theme	high-mannose-type	1201:1217	arg1	N-glycans					1219:1227	only the high-mannose-type N-glycans	1192:1227	only the high-mannose-type N-glycans	1192:1227	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	4	4	with	protein	703:709	arg1	N-glycans					741:749	mainly high-mannose-type N-glycans	716:749	mainly high-mannose-type N-glycans	716:749	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	1	5	theme	novel	178:182	arg1	therapies					184:192	novel therapies	178:192	novel therapies	178:192	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	4	6	from	MAN1B1	602:607	arg1	cells					620:624	HEK293 cells	613:624	HEK293 cells	613:624	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	6	7	theme	recombinant	922:932	arg1	proteins					934:941	Two recombinant proteins	918:941	Two recombinant proteins	918:941	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	7	theme	recombinant	922:932	arg1	lipase					959:964	lysosomal acid lipase	944:964	lysosomal acid lipase (LIPA)	944:971	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	7	theme	recombinant	922:932	arg1	G1					992:993	immunoglobulin G1	977:993	immunoglobulin G1 (IgG1)	977:1000	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	7	8	theme	Glycan	1083:1088	arg1	analysis					1101:1108	Glycan structural analysis	1083:1108	Glycan structural analysis	1083:1108	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	9	9	theme	therapeutic	1538:1548	arg1	proteins					1550:1557	homogeneous therapeutic proteins	1526:1557	homogeneous therapeutic proteins	1526:1557	Our results suggest that these cell lines could be suitable platforms to produce homogeneous therapeutic proteins.					
31102532	3	10	gly	heterogeneity	404:416	arg1	structures					428:437	glycan structures	421:437	glycan structures	421:437	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	2	11	theme	cell	301:304	arg1	line					306:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	9	12	theme	suitable	1496:1503	arg1	platforms					1505:1513	suitable platforms	1496:1513	suitable platforms	1496:1513	Our results suggest that these cell lines could be suitable platforms to produce homogeneous therapeutic proteins.					
31102532	4	13	from	MAN1A2	591:596	arg1	cells					620:624	HEK293 cells	613:624	HEK293 cells	613:624	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	6	14	theme	cell	1071:1074	arg1	lines					1076:1080	cell lines	1071:1080	cell lines	1071:1080	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	1	15	theme	Therapeutic	83:93	arg1	proteins					95:102	Therapeutic proteins	83:102	Therapeutic proteins	83:102	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	15	theme	Therapeutic	83:93	arg1	part					121:124	a developing part	108:124	a developing part of the modern biopharmaceutical industry	108:165	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	4	16	theme	cell	665:668	arg1	line					670:673	a triple-knockout (T-KO) cell line	640:673	a triple-knockout (T-KO) cell line	640:673	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	4	17	from	MAN1A1	583:588	arg1	cells					620:624	HEK293 cells	613:624	HEK293 cells	613:624	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	7	18	theme	QD-KO	1292:1296	arg1	cells					1308:1312	the QD-KO and QT-KO cells	1288:1312	cells	1308:1312	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	7	19	theme	recombinant	1253:1263	arg1	proteins					1265:1272	the recombinant proteins	1249:1272	the recombinant proteins prepared from the QD-KO and QT-KO cells	1249:1312	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	1	20	theme	intractable	197:207	arg1	cancers					228:234	cancers	228:234	cancers	228:234	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	20	theme	intractable	197:207	arg1	diseases					251:258	autoimmune diseases	240:258	autoimmune diseases	240:258	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	20	theme	intractable	197:207	arg1	diseases					209:216	intractable diseases	197:216	intractable diseases including cancers and autoimmune diseases	197:258	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	4	21	theme	high-mannose-type	723:739	arg1	N-glycans					741:749	mainly high-mannose-type N-glycans	716:749	mainly high-mannose-type N-glycans	716:749	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	0	22	theme	cells	27:31	arg1	Glycoengineering					0:15	Glycoengineering	0:15	Glycoengineering of HEK293 cells	0:31	Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures.					
31102532	6	23	theme	immunoglobulin	977:990	arg1	proteins					934:941	Two recombinant proteins	918:941	Two recombinant proteins	918:941	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	23	theme	immunoglobulin	977:990	arg1	IgG1					996:999	IgG1	996:999	IgG1	996:999	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	23	theme	immunoglobulin	977:990	arg1	G1					992:993	immunoglobulin G1	977:993	immunoglobulin G1 (IgG1)	977:1000	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	7	24	theme	structural	1090:1099	arg1	analysis					1101:1108	Glycan structural analysis	1083:1108	Glycan structural analysis	1083:1108	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	1	25	theme	developing	110:119	arg1	proteins					95:102	Therapeutic proteins	83:102	Therapeutic proteins	83:102	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	25	theme	developing	110:119	arg1	part					121:124	a developing part	108:124	a developing part of the modern biopharmaceutical industry	108:165	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	4	26	dep	triple-knockout	642:656	arg1	T-KO					659:662	T-KO	659:662	T-KO	659:662	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	3	27	theme	issues	470:475	arg1	issues					470:475	the most challenging issues	449:475	the most challenging issues in the production of therapeutic proteins	449:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	27	theme	issues	470:475	arg1	one					442:444	one	442:444	one	442:444	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	27	theme	issues	470:475	arg1	heterogeneity					404:416	The heterogeneity	400:416	The heterogeneity of glycan structures	400:437	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	0	28	theme	HEK293	20:25	arg1	cells					27:31	HEK293 cells	20:31	HEK293 cells	20:31	Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures.					
31102532	6	29	dep	quadruple-KO	1025:1036	arg1	lines					1076:1080	cell lines	1071:1080	cell lines	1071:1080	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	8	30	theme	slow	1374:1377	arg1	growth					1379:1384	the slow growth	1370:1384	the slow growth in QD-KO and QT-KO	1370:1403	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	9	31	theme	homogeneous	1526:1536	arg1	proteins					1550:1557	homogeneous therapeutic proteins	1526:1557	homogeneous therapeutic proteins	1526:1557	Our results suggest that these cell lines could be suitable platforms to produce homogeneous therapeutic proteins.					
31102532	5	32	theme	Golgi	815:819	arg1	α1,2-mannosidase-I					821:838	another Golgi α1,2-mannosidase-I	807:838	another Golgi α1,2-mannosidase-I	807:838	Here, we further knocked out MAN1C1 and MGAT1 encoding another Golgi α1,2-mannosidase-I and N-acetylglucosaminyltransferase-I, respectively, based on the T-KO cells.					
31102532	3	33	theme	glycan	421:426	arg1	structures					428:437	glycan structures	421:437	glycan structures	421:437	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	34	theme	therapeutic	498:508	arg1	proteins					510:517	therapeutic proteins	498:517	therapeutic proteins	498:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	4	35	theme	triple-knockout	642:656	arg1	line					670:673	a triple-knockout (T-KO) cell line	640:673	a triple-knockout (T-KO) cell line	640:673	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	2	36	theme	human	265:269	arg1	line					306:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	3	37	theme	most	453:456	arg1	issues					470:475	the most challenging issues	449:475	the most challenging issues in the production of therapeutic proteins	449:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	38	theme	structures	428:437	arg1	issues					470:475	the most challenging issues	449:475	the most challenging issues in the production of therapeutic proteins	449:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	38	theme	structures	428:437	arg1	one					442:444	one	442:444	one	442:444	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	3	38	theme	structures	428:437	arg1	heterogeneity					404:416	The heterogeneity	400:416	The heterogeneity of glycan structures	400:437	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	7	39	theme	hybrid-type	1132:1142	arg1	N-glycans					1161:1169	all the hybrid-type and complex-type N-glycans	1124:1169	all the hybrid-type and complex-type N-glycans	1124:1169	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	4	40	theme	recombinant	691:701	arg1	protein					703:709	recombinant protein	691:709	recombinant protein with mainly high-mannose-type N-glycans	691:749	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	4	41	from	α1,2-mannosidase-Is	562:580	arg1	cells					620:624	HEK293 cells	613:624	HEK293 cells	613:624	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
31102532	1	42	theme	autoimmune	240:249	arg1	diseases					251:258	autoimmune diseases	240:258	autoimmune diseases	240:258	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	0	43	theme	N-glycan	62:69	arg1	structures					71:80	high-mannose-type N-glycan structures	44:80	high-mannose-type N-glycan structures	44:80	Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures.					
31102532	5	44	theme	T-KO	906:909	arg1	cells					911:915	the T-KO cells	902:915	the T-KO cells	902:915	Here, we further knocked out MAN1C1 and MGAT1 encoding another Golgi α1,2-mannosidase-I and N-acetylglucosaminyltransferase-I, respectively, based on the T-KO cells.					
31102532	0	45	theme	high-mannose-type	44:60	arg1	structures					71:80	high-mannose-type N-glycan structures	44:80	high-mannose-type N-glycan structures	44:80	Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures.					
31102532	3	46	from	issues	470:475	arg1	production					484:493	the production	480:493	the production of therapeutic proteins	480:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	2	47	theme	recombinant	343:353	arg1	proteins					355:362	recombinant proteins	343:362	recombinant proteins	343:362	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	6	48	theme	acid	954:957	arg1	proteins					934:941	Two recombinant proteins	918:941	Two recombinant proteins	918:941	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	48	theme	acid	954:957	arg1	LIPA					967:970	LIPA	967:970	LIPA	967:970	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	48	theme	acid	954:957	arg1	lipase					959:964	lysosomal acid lipase	944:964	lysosomal acid lipase (LIPA)	944:971	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	1	49	theme	modern	133:138	arg1	industry					158:165	the modern biopharmaceutical industry	129:165	the modern biopharmaceutical industry	129:165	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	50	theme	biopharmaceutical	140:156	arg1	industry					158:165	the modern biopharmaceutical industry	129:165	the modern biopharmaceutical industry	129:165	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	8	51	theme	oncogenes	1337:1345	arg1	Overexpression					1315:1328	Overexpression	1315:1328	Overexpression of the oncogenes MYC and MYCN	1315:1358	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	9	52	theme	cell	1476:1479	arg1	lines					1481:1485	these cell lines	1470:1485	these cell lines	1470:1485	Our results suggest that these cell lines could be suitable platforms to produce homogeneous therapeutic proteins.					
31102532	3	53	theme	challenging	458:468	arg1	issues					470:475	the most challenging issues	449:475	the most challenging issues in the production of therapeutic proteins	449:517	The heterogeneity of glycan structures is one of the most challenging issues in the production of therapeutic proteins.					
31102532	2	54	theme	kidney	281:286	arg1	line					306:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	7	55	theme	QT-KO	1302:1306	arg1	cells					1308:1312	the QD-KO and QT-KO cells	1288:1312	cells	1308:1312	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	1	56	theme	industry	158:165	arg1	proteins					95:102	Therapeutic proteins	83:102	Therapeutic proteins	83:102	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	1	56	theme	industry	158:165	arg1	part					121:124	a developing part	108:124	a developing part of the modern biopharmaceutical industry	108:165	Therapeutic proteins are a developing part of the modern biopharmaceutical industry, providing novel therapies to intractable diseases including cancers and autoimmune diseases.					
31102532	2	57	used	used	327:330	arg2	line					306:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	6	58	theme	lysosomal	944:952	arg1	proteins					934:941	Two recombinant proteins	918:941	Two recombinant proteins	918:941	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	58	theme	lysosomal	944:952	arg1	LIPA					967:970	LIPA	967:970	LIPA	967:970	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	6	58	theme	lysosomal	944:952	arg1	lipase					959:964	lysosomal acid lipase	944:964	lysosomal acid lipase (LIPA)	944:971	Two recombinant proteins, lysosomal acid lipase (LIPA) and immunoglobulin G1 (IgG1), were expressed in the quadruple-KO (QD-KO) and quintuple-KO (QT-KO) cell lines.					
31102532	2	59	theme	embryonic	271:279	arg1	kidney					281:286	embryonic kidney 293	271:290	The human embryonic kidney 293 (HEK293) cell line	261:309	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	2	59	theme	embryonic	271:279	arg1	HEK293					293:298	HEK293	293:298	HEK293	293:298	The human embryonic kidney 293 (HEK293) cell line has been widely used to produce recombinant proteins in both basic science and industry.					
31102532	7	60	theme	complex-type	1148:1159	arg1	N-glycans					1161:1169	all the hybrid-type and complex-type N-glycans	1124:1169	all the hybrid-type and complex-type N-glycans	1124:1169	Glycan structural analysis revealed that all the hybrid-type and complex-type N-glycans were eliminated, and only the high-mannose-type N-glycans were detected among the recombinant proteins prepared from the QD-KO and QT-KO cells.					
31102532	8	61	from	growth	1379:1384	arg1	QT-KO					1399:1403	QT-KO	1399:1403	QT-KO	1399:1403	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	8	61	from	growth	1379:1384	arg1	QD-KO					1389:1393	QD-KO	1389:1393	QD-KO	1389:1393	Overexpression of the oncogenes MYC and MYCN recovered the slow growth in QD-KO and QT-KO without changing the glycan structures.					
31102532	4	62	theme	HEK293	613:618	arg1	cells					620:624	HEK293 cells	613:624	HEK293 cells	613:624	Previously, we knocked out genes encoding α1,2-mannosidase-Is, MAN1A1, MAN1A2 and MAN1B1, in HEK293 cells, establishing a triple-knockout (T-KO) cell line, which produced recombinant protein with mainly high-mannose-type N-glycans.					
30915686	5	0	theme	group	737:741	arg1	model					699:703	a model	697:703	a model of depression, and (ii) control group	697:741	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	4	1	theme	depressive-like	530:544	arg1	behavior					546:553	depressive-like behavior	530:553	depressive-like behavior using a stress based mouse model	530:586	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	5	2	theme	chronic	653:659	arg1	CUMS					688:691	CUMS	688:691	CUMS	688:691	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	5	2	theme	chronic	653:659	arg1	stress					680:685	chronic unpredictable mild stress	653:685	chronic unpredictable mild stress (CUMS)	653:692	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	7	3	from	changes	1170:1176	arg1	profile					1195:1201	the N-glycome profile	1181:1201	the N-glycome profile	1181:1201	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	8	4	theme	depressive-like	1260:1274	arg1	model					1276:1280	the depressive-like model	1256:1280	the depressive-like model	1256:1280	N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data.					
30915686	5	5	used	used	592:595	arg2	We					589:590	We	589:590	We	589:590	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	9	6	theme	depressive	1421:1430	arg1	symptoms					1432:1439	depressive symptoms	1421:1439	depressive symptoms	1421:1439	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	0	7	theme	stress	85:90	arg1	response					92:99	chronic stress response	77:99	chronic stress response	77:99	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	5	8	theme	unpredictable	661:673	arg1	CUMS					688:691	CUMS	688:691	CUMS	688:691	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	5	8	theme	unpredictable	661:673	arg1	stress					680:685	chronic unpredictable mild stress	653:685	chronic unpredictable mild stress (CUMS)	653:692	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	6	9	theme	sucrose	776:782	arg1	test					795:798	sucrose preference test	776:798	sucrose preference test	776:798	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	9	theme	sucrose	776:782	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	10	theme	swimming	808:815	arg1	test					817:820	forced swimming test	801:820	forced swimming test	801:820	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	10	theme	swimming	808:815	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	7	11	theme	N-glycome	1185:1193	arg1	profile					1195:1201	the N-glycome profile	1181:1201	the N-glycome profile	1181:1201	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	5	12	theme	mild	675:678	arg1	CUMS					688:691	CUMS	688:691	CUMS	688:691	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	5	12	theme	mild	675:678	arg1	stress					680:685	chronic unpredictable mild stress	653:685	chronic unpredictable mild stress (CUMS)	653:692	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	8	13	theme	expression	1213:1222	arg1	levels					1224:1229	N-glycan expression levels	1204:1229	N-glycan expression levels	1204:1229	N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data.					
30915686	9	14	dep	diagnosis	1507:1515	arg1	the					1494:1496	the	1494:1496	the	1494:1496	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	6	15	used	used	856:859	arg2	test					817:820	forced swimming test	801:820	forced swimming test	801:820	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	15	used	used	856:859	arg2	test					795:798	sucrose preference test	776:798	sucrose preference test	776:798	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	15	used	used	856:859	arg2	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	15	used	used	856:859	arg2	test					845:848	fear conditioning test	827:848	fear conditioning test	827:848	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	1	16	from	modification	156:167	arg1	eukaryotes					172:181	eukaryotes	172:181	eukaryotes	172:181	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	2	17	theme	biomarker	297:305	arg1	discovery					307:315	biomarker discovery	297:315	biomarker discovery of neuropsychiatric disorders	297:345	N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders.					
30915686	6	18	dep	tests	755:759	arg1	test					845:848	fear conditioning test	827:848	fear conditioning test	827:848	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	18	dep	tests	755:759	arg1	test					817:820	forced swimming test	801:820	forced swimming test	801:820	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	18	dep	tests	755:759	arg1	test					795:798	sucrose preference test	776:798	sucrose preference test	776:798	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	18	dep	tests	755:759	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	19	theme	depressive-like	919:933	arg1	phenotype					935:943	a depressive-like phenotype	917:943	a depressive-like phenotype	917:943	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	5	20	theme	treated	628:634	arg1	group					636:640	(i) treated group	624:640	(i) treated group	624:640	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	7	21	dep	using	986:990	arg1	illustrate					1155:1164	illustrate	1155:1164	to illustrate the changes in the N-glycome profile	1152:1201	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	7	21	dep	using	986:990	arg1	exhibit					1113:1119	exhibit	1113:1119	to exhibit N-glycan expression levels	1110:1146	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	9	22	gly	sialylated	1355:1364	arg1	biomarker					1395:1403	a biomarker	1393:1403	a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression	1393:1544	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	22	gly	sialylated	1355:1364	arg1	N-glycan					1366:1373	sialylated N-glycan	1355:1373	sialylated N-glycan	1355:1373	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	7	23	theme	spectrometry	1082:1093	arg1	ionization-time					1051:1065	Matrix-assisted laser desorption ionization-time	1018:1065	Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS)	1018:1108	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	3	24	theme	N-glycosylation	382:396	arg1	pattern					398:404	N-glycosylation pattern	382:404	N-glycosylation pattern	382:404	However, the relationship between N-glycosylation pattern and depression is not well elucidated to date.					
30915686	6	25	theme	conditioning	832:843	arg1	test					845:848	fear conditioning test	827:848	fear conditioning test	827:848	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	25	theme	conditioning	832:843	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	9	26	theme	clinical	1498:1505	arg1	diagnosis					1507:1515	clinical diagnosis	1498:1515	clinical diagnosis	1498:1515	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	27	theme	depression	1535:1544	arg1	diagnosis					1507:1515	clinical diagnosis	1498:1515	clinical diagnosis	1498:1515	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	27	theme	depression	1535:1544	arg1	monitoring					1521:1530	monitoring	1521:1530	monitoring	1521:1530	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	6	28	theme	fear	827:830	arg1	conditioning					832:843	fear conditioning	827:843	fear conditioning test	827:848	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	1	29	theme	important	196:204	arg1	role					206:209	an important role	193:209	an important role	193:209	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	6	30	dep	test	795:798	arg1	e.g.					770:773	e.g.	770:773	e.g.	770:773	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	7	31	theme	flight/mass	1070:1080	arg1	spectrometry					1082:1093	flight/mass spectrometry	1070:1093	flight/mass spectrometry (MALDI-TOF/MS)	1070:1108	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	7	31	theme	flight/mass	1070:1080	arg1	MALDI-TOF/MS					1096:1107	MALDI-TOF/MS	1096:1107	MALDI-TOF/MS	1096:1107	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	5	32	theme	BALB/c	611:616	arg1	mice					618:621	BALB/c mice	611:621	BALB/c mice	611:621	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	2	33	theme	disorders	337:345	arg1	discovery					307:315	biomarker discovery	297:315	biomarker discovery of neuropsychiatric disorders	297:345	N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders.					
30915686	0	34	theme	N-glycoproteins	24:38	arg1	Identification					0:13	Identification	0:13	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.	0:108	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	5	35	dep	group	737:741	arg1	ii					725:726	ii	725:726	ii	725:726	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	5	36	theme	mice	618:621	arg1	groups					601:606	two groups	597:606	two groups of BALB/c mice	597:621	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	5	36	theme	mice	618:621	arg1	mice					618:621	BALB/c mice	611:621	BALB/c mice	611:621	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	2	37	theme	neuropsychiatric	320:335	arg1	disorders					337:345	neuropsychiatric disorders	320:345	neuropsychiatric disorders	320:345	N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders.					
30915686	6	38	from	tests	755:759	arg1	mice					764:767	mice	764:767	mice	764:767	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	0	39	theme	serum	18:22	arg1	N-glycoproteins					24:38	serum N-glycoproteins	18:38	serum N-glycoproteins	18:38	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	9	40	theme	candidate	1470:1478	arg1	utility					1457:1463	utility	1457:1463	utility	1457:1463	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	40	theme	candidate	1470:1478	arg1	biomarker					1480:1488	a candidate biomarker	1468:1488	a candidate biomarker for the clinical diagnosis and monitoring of depression	1468:1544	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	8	41	theme	N-glycan	1204:1211	arg1	levels					1224:1229	N-glycan expression levels	1204:1229	N-glycan expression levels	1204:1229	N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data.					
30915686	9	42	theme	sialylated	1355:1364	arg1	biomarker					1395:1403	a biomarker	1393:1403	a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression	1393:1544	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	42	theme	sialylated	1355:1364	arg1	N-glycan					1366:1373	sialylated N-glycan	1355:1373	sialylated N-glycan	1355:1373	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	7	43	theme	Matrix-assisted	1018:1032	arg1	ionization-time					1051:1065	Matrix-assisted laser desorption ionization-time	1018:1065	Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS)	1018:1108	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	1	44	theme	several	226:232	arg1	diseases					234:241	several diseases	226:241	several diseases	226:241	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	4	45	theme	based	570:574	arg1	model					582:586	a stress based mouse model	561:586	a stress based mouse model	561:586	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	5	46	theme	depression	708:717	arg1	model					699:703	a model	697:703	a model of depression, and (ii) control group	697:741	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	4	47	theme	N-glycan	495:502	arg1	structures					504:513	serum N-glycan structures	489:513	serum N-glycan structures	489:513	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	7	48	theme	N-glycan	1121:1128	arg1	levels					1141:1146	N-glycan expression levels	1121:1146	N-glycan expression levels	1121:1146	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	4	49	theme	stress	563:568	arg1	model					582:586	a stress based mouse model	561:586	a stress based mouse model	561:586	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	4	50	theme	serum	489:493	arg1	structures					504:513	serum N-glycan structures	489:513	serum N-glycan structures	489:513	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	2	51	theme	N-glycan	244:251	arg1	structure					253:261	N-glycan structure	244:261	N-glycan structure	244:261	N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders.					
30915686	6	52	theme	Behavioral	744:753	arg1	test					845:848	fear conditioning test	827:848	fear conditioning test	827:848	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	52	theme	Behavioral	744:753	arg1	test					817:820	forced swimming test	801:820	forced swimming test	801:820	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	52	theme	Behavioral	744:753	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	52	theme	Behavioral	744:753	arg1	test					795:798	sucrose preference test	776:798	sucrose preference test	776:798	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	7	53	theme	laser	1034:1038	arg1	ionization-time					1051:1065	Matrix-assisted laser desorption ionization-time	1018:1065	Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS)	1018:1108	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	0	54	theme	biological	45:54	arg1	correlate					56:64	a biological correlate	43:64	a biological correlate underlying chronic stress response in mice	43:107	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	6	55	theme	threshold	877:885	arg1	level					887:891	the threshold level	873:891	the threshold level to which mice displayed a depressive-like phenotype	873:943	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	7	56	theme	desorption	1040:1049	arg1	ionization-time					1051:1065	Matrix-assisted laser desorption ionization-time	1018:1065	Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS)	1018:1108	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	6	57	theme	forced	801:806	arg1	test					817:820	forced swimming test	801:820	forced swimming test	801:820	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	57	theme	forced	801:806	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	0	58	gly	N-glycoproteins	24:38	arg1	N-glycoproteins					24:38	serum N-glycoproteins	18:38	serum N-glycoproteins	18:38	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	9	59	contain	have	1452:1455	arg1	symptoms					1432:1439	depressive symptoms	1421:1439	depressive symptoms	1421:1439	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	59	contain	have	1452:1455	arg2	biomarker					1480:1488	a candidate biomarker	1468:1488	a candidate biomarker for the clinical diagnosis and monitoring of depression	1468:1544	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	9	59	contain	have	1452:1455	arg2	utility					1457:1463	utility	1457:1463	utility	1457:1463	Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.					
30915686	4	60	theme	mouse	576:580	arg1	model					582:586	a stress based mouse model	561:586	a stress based mouse model	561:586	This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model.					
30915686	7	61	theme	expression	1130:1139	arg1	levels					1141:1146	N-glycan expression levels	1121:1146	N-glycan expression levels	1121:1146	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	2	62	theme	new	280:282	arg1	paradigm					284:291	a new paradigm	278:291	a new paradigm for biomarker discovery of neuropsychiatric disorders	278:345	N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders.					
30915686	1	63	theme	post-translational	129:146	arg1	modification					156:167	a post-translational protein modification	127:167	a post-translational protein modification	127:167	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	1	63	theme	post-translational	129:146	arg1	Glycosylation					110:122	Glycosylation	110:122	Glycosylation	110:122	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	5	64	dep	group	636:640	arg1	i					625:625	i	625:625	i	625:625	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	1	65	theme	protein	148:154	arg1	modification					156:167	a post-translational protein modification	127:167	a post-translational protein modification	127:167	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	1	65	theme	protein	148:154	arg1	Glycosylation					110:122	Glycosylation	110:122	Glycosylation	110:122	Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases.					
30915686	0	66	theme	chronic	77:83	arg1	response					92:99	chronic stress response	77:99	chronic stress response	77:99	Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice.					
30915686	6	67	theme	preference	784:793	arg1	test					795:798	sucrose preference test	776:798	sucrose preference test	776:798	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	6	67	theme	preference	784:793	arg1	tests					755:759	Behavioral tests	744:759	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test)	744:849	Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype.					
30915686	7	68	theme	Serum	946:950	arg1	N-glycans					952:960	Serum N-glycans	946:960	Serum N-glycans	946:960	Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile.					
30915686	5	69	theme	control	729:735	arg1	group					737:741	(ii) control group	724:741	(ii) control group	724:741	We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group.					
30915686	8	70	theme	behavioral	1311:1320	arg1	data					1322:1325	the behavioral data	1307:1325	the behavioral data	1307:1325	N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data.					
30759204	11	0	theme	synthesis	1385:1393	arg1	exploration					1403:1413	synthesis pathway exploration	1385:1413	synthesis pathway exploration	1385:1413	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	10	1	theme	follow-up	1236:1244	arg1	pathways					1246:1253	multiple start-sites and follow-up pathways	1211:1253	multiple start-sites and follow-up pathways	1211:1253	Our data demonstrated that multiple start-sites and follow-up pathways were key to increasing the number of glycans added.					
30759204	8	2	theme	sites	1010:1014	arg1	selection					984:992	selection	984:992	selection of four initial sites based on amino-acid sequence recognition	984:1055	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	1	3	from	manner	199:204	arg1	regions					216:222	repeat regions	209:222	repeat regions of proteins, such as mucins and IgA1	209:259	GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1.					
30759204	11	4	theme	HR	1353:1354	arg1	density					1363:1369	IgA1 HR glycan density	1348:1369	IgA1 HR glycan density	1348:1369	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	10	5	theme	start-sites	1220:1230	arg1	pathways					1246:1253	multiple start-sites and follow-up pathways	1211:1253	multiple start-sites and follow-up pathways	1211:1253	Our data demonstrated that multiple start-sites and follow-up pathways were key to increasing the number of glycans added.					
30759204	11	6	theme	pathway	1395:1401	arg1	exploration					1403:1413	synthesis pathway exploration	1385:1413	synthesis pathway exploration	1385:1413	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	8	7	theme	initial	1002:1008	arg1	sites					1010:1014	four initial sites	997:1014	four initial sites based on amino-acid sequence recognition	997:1055	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	10	8	theme	multiple	1211:1218	arg1	pathways					1246:1253	multiple start-sites and follow-up pathways	1211:1253	multiple start-sites and follow-up pathways	1211:1253	Our data demonstrated that multiple start-sites and follow-up pathways were key to increasing the number of glycans added.					
30759204	6	9	theme	hinge-region	802:813	arg1	segment					820:826	IgA1 hinge-region (HR) segment	797:826	IgA1 hinge-region (HR) segment	797:826	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	6	9	theme	hinge-region	802:813	arg1	probe					833:837	a probe	831:837	a probe	831:837	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	9	10	theme	initial	1160:1166	arg1	site-selection					1168:1181	initial site-selection	1160:1181	initial site-selection	1160:1181	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	12	11	theme	O-glycosylation	1574:1588	arg1	patterns					1590:1597	conserved clustered O-glycosylation patterns	1554:1597	conserved clustered O-glycosylation patterns	1554:1597	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	9	12	theme	multiple	1109:1116	arg1	site-selections					1125:1139	multiple second site-selections	1109:1139	multiple second site-selections	1109:1139	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	9	12	theme	multiple	1109:1116	arg1	each					1142:1145	each	1142:1145	each	1142:1145	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	3	13	dep	sites	389:393	arg1	the					385:387	the	385:387	the	385:387	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	5	14	theme	O-glycan	599:606	arg1	structures					608:617	O-glycan structures	599:617	O-glycan structures	599:617	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	12	15	theme	clustered	1564:1572	arg1	patterns					1590:1597	conserved clustered O-glycosylation patterns	1554:1597	conserved clustered O-glycosylation patterns	1554:1597	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	8	16	theme	sequence	1036:1043	arg1	recognition					1045:1055	amino-acid sequence recognition	1025:1055	amino-acid sequence recognition	1025:1055	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	6	17	theme	IgA1	797:800	arg1	hinge-region					802:813	IgA1 hinge-region	797:813	IgA1 hinge-region (HR) segment	797:826	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	6	17	theme	IgA1	797:800	arg1	HR					816:817	HR	816:817	HR	816:817	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	7	18	theme	semi-ordered	871:882	arg1	process					884:890	a semi-ordered process	869:890	a semi-ordered process of glycosylation	869:907	Using LC-MS, we demonstrated a semi-ordered process of glycosylation by GalNAc-T2 towards the IgA1 HR.					
30759204	1	19	theme	clustered	189:197	arg1	manner					199:204	a clustered manner	187:204	a clustered manner in repeat regions of proteins, such as mucins and IgA1	187:259	GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1.					
30759204	13	20	theme	glycan	1862:1867	arg1	heterogeneity					1869:1881	glycan heterogeneity	1862:1881	glycan heterogeneity in a disease-relevant context	1862:1911	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	5	21	theme	IgA1	622:625	arg1	hinge-region					627:638	IgA1 hinge-region	622:638	IgA1 hinge-region	622:638	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	5	22	theme	autoimmune	565:574	arg1	nephropathy					549:559	IgA nephropathy	545:559	IgA nephropathy	545:559	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	5	22	theme	autoimmune	565:574	arg1	disease					576:582	an autoimmune disease	562:582	an autoimmune disease	562:582	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	2	23	theme	glycosylation	276:288	arg1	patterns					290:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	0	24	theme	hinge-region	5:16	arg1	fidelity					35:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.					
30759204	13	25	theme	disease-relevant	1888:1903	arg1	context					1905:1911	a disease-relevant context	1886:1911	a disease-relevant context	1886:1911	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	5	26	theme	hinge-region	627:638	arg1	hinge-region					627:638	IgA1 hinge-region	622:638	IgA1 hinge-region	622:638	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	5	26	theme	hinge-region	627:638	arg1	sites					589:593	sites	589:593	sites	589:593	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	5	26	theme	hinge-region	627:638	arg1	structures					608:617	O-glycan structures	599:617	O-glycan structures	599:617	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	2	27	theme	similar	326:332	arg1	sites					334:338	similar sites	326:338	similar sites with similar structures	326:362	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	9	28	theme	lectin	1077:1082	arg1	domains					1084:1090	lectin domains	1077:1090	lectin domains	1077:1090	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	0	29	theme	IgA1	0:3	arg1	fidelity					35:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.					
30759204	12	30	theme	site-specific	1463:1475	arg1	addition					1484:1491	site-specific glycan addition	1463:1491	site-specific glycan addition	1463:1491	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	11	31	theme	lectin	1311:1316	arg1	domain					1318:1323	The lectin domain	1307:1323	The lectin domain	1307:1323	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	1	32	theme	repeat	209:214	arg1	regions					216:222	repeat regions	209:222	repeat regions of proteins, such as mucins and IgA1	209:259	GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1.					
30759204	0	33	theme	glycan	28:33	arg1	fidelity					35:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.					
30759204	7	34	theme	IgA1	934:937	arg1	HR					939:940	the IgA1 HR	930:940	the IgA1 HR	930:940	Using LC-MS, we demonstrated a semi-ordered process of glycosylation by GalNAc-T2 towards the IgA1 HR.					
30759204	6	35	theme	O-glycans	778:786	arg1	densities					755:763	densities	755:763	densities	755:763	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	6	35	theme	O-glycans	778:786	arg1	O-glycans					778:786	clustered O-glycans	768:786	clustered O-glycans	768:786	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	6	35	theme	O-glycans	778:786	arg1	sites					745:749	sites	745:749	sites	745:749	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	1	36	theme	GalNAc-type	113:123	arg1	O-glycans					125:133	GalNAc-type O-glycans	113:133	GalNAc-type O-glycans	113:133	GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1.					
30759204	13	37	theme	glycosylation	1726:1738	arg1	diversity					1748:1756	glycosylation pathway diversity	1726:1756	glycosylation pathway diversity	1726:1756	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	0	38	theme	clustered	18:26	arg1	fidelity					35:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity	0:42	IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.					
30759204	10	39	theme	glycans	1292:1298	arg1	number					1282:1287	the number	1278:1287	the number of glycans added	1278:1304	Our data demonstrated that multiple start-sites and follow-up pathways were key to increasing the number of glycans added.					
30759204	12	40	theme	conserved	1554:1562	arg1	patterns					1590:1597	conserved clustered O-glycosylation patterns	1554:1597	conserved clustered O-glycosylation patterns	1554:1597	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	3	41	theme	conservative	464:475	arg1	process					477:483	a conservative process	462:483	a conservative process	462:483	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	2	42	with	sites	334:338	arg1	structures					353:362	similar structures	345:362	similar structures	345:362	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	6	43	used	used	792:795	arg2	we					789:790	we	789:790	we	789:790	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	1	44	theme	proteins	227:234	arg1	regions					216:222	repeat regions	209:222	repeat regions of proteins, such as mucins and IgA1	209:259	GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1.					
30759204	3	45	theme	glycans	409:415	arg1	number					399:404	number	399:404	number	399:404	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	3	45	theme	glycans	409:415	arg1	sites					389:393	sites	389:393	sites	389:393	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	3	45	theme	glycans	409:415	arg1	proteins					441:448	other proteins	435:448	other proteins	435:448	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	3	45	theme	glycans	409:415	arg1	glycans					409:415	glycans	409:415	glycans added to IgA1	409:429	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	12	46	theme	IgA1	1628:1631	arg1	patterns					1590:1597	conserved clustered O-glycosylation patterns	1554:1597	conserved clustered O-glycosylation patterns	1554:1597	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	12	46	theme	IgA1	1628:1631	arg1	populations					1613:1623	glycoform populations	1603:1623	glycoform populations	1603:1623	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	13	47	theme	GalNAc-T	1824:1831	arg1	isozyme					1833:1839	a single GalNAc-T isozyme	1815:1839	a single GalNAc-T isozyme	1815:1839	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	6	48	theme	clustered	768:776	arg1	O-glycans					778:786	clustered O-glycans	768:786	clustered O-glycans	768:786	To better understand how GalNAc-transferases determine sites and densities of clustered O-glycans, we used IgA1 hinge-region (HR) segment as a probe.					
30759204	5	49	theme	IgA	545:547	arg1	nephropathy					549:559	IgA nephropathy	545:559	IgA nephropathy	545:559	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	5	49	theme	IgA	545:547	arg1	disease					576:582	an autoimmune disease	562:582	an autoimmune disease	562:582	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	3	50	theme	other	435:439	arg1	proteins					441:448	other proteins	435:448	other proteins	435:448	It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process.					
30759204	11	51	theme	glycan	1356:1361	arg1	density					1363:1369	IgA1 HR glycan density	1348:1369	IgA1 HR glycan density	1348:1369	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	4	52	theme	GalNAc-type	515:525	arg1	glycosylation					527:539	GalNAc-type glycosylation	515:539	GalNAc-type glycosylation	515:539	GalNAc-transferases initiate GalNAc-type glycosylation.					
30759204	13	53	theme	glycosylation	1762:1774	arg1	density					1776:1782	glycosylation density	1762:1782	glycosylation density	1762:1782	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	9	54	theme	second	1118:1123	arg1	site-selections					1125:1139	multiple second site-selections	1109:1139	multiple second site-selections	1109:1139	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	9	54	theme	second	1118:1123	arg1	each					1142:1145	each	1142:1145	each	1142:1145	Both catalytic and lectin domains were involved in multiple second site-selections, each dependent on initial site-selection.					
30759204	12	55	theme	glycan	1507:1512	arg1	density					1514:1520	clustered glycan density	1497:1520	clustered glycan density	1497:1520	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	8	56	theme	amino-acid	1025:1034	arg1	recognition					1045:1055	amino-acid sequence recognition	1025:1055	amino-acid sequence recognition	1025:1055	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	2	57	theme	similar	345:351	arg1	structures					353:362	similar structures	345:362	similar structures	345:362	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	5	58	theme	glycan	670:675	arg1	autoantigen					677:687	a glycan autoantigen	668:687	a glycan autoantigen	668:687	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	12	59	theme	clustered	1497:1505	arg1	density					1514:1520	clustered glycan density	1497:1520	clustered glycan density	1497:1520	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	2	60	theme	IgA1	271:274	arg1	patterns					290:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	8	61	theme	catalytic	947:955	arg1	responsible					968:978	responsible	968:978	responsible	968:978	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	8	61	theme	catalytic	947:955	arg1	domain					957:962	The catalytic domain	943:962	The catalytic domain	943:962	The catalytic domain was responsible for selection of four initial sites based on amino-acid sequence recognition.					
30759204	7	62	theme	glycosylation	895:907	arg1	process					884:890	a semi-ordered process	869:890	a semi-ordered process of glycosylation	869:907	Using LC-MS, we demonstrated a semi-ordered process of glycosylation by GalNAc-T2 towards the IgA1 HR.					
30759204	13	63	from	heterogeneity	1869:1881	arg1	context					1905:1911	a disease-relevant context	1886:1911	a disease-relevant context	1886:1911	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	2	64	theme	Observed	262:269	arg1	patterns					290:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns	262:297	Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures.					
30759204	5	65	dep	sites	589:593	arg1	the					585:587	the	585:587	the	585:587	In IgA nephropathy, an autoimmune disease, the sites and O-glycan structures of IgA1 hinge-region are altered, giving rise to a glycan autoantigen.					
30759204	0	66	theme	semi-ordered	72:83	arg1	glycosylation					85:97	semi-ordered glycosylation	72:97	semi-ordered glycosylation	72:97	IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.					
30759204	13	67	theme	single	1817:1822	arg1	isozyme					1833:1839	a single GalNAc-T isozyme	1815:1839	a single GalNAc-T isozyme	1815:1839	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	13	68	theme	pathway	1740:1746	arg1	diversity					1748:1756	glycosylation pathway diversity	1726:1756	glycosylation pathway diversity	1726:1756	Together, these findings characterized a correlation between glycosylation pathway diversity and glycosylation density, revealing mechanisms by which a single GalNAc-T isozyme can limit and define glycan heterogeneity in a disease-relevant context.					
30759204	12	69	theme	glycan	1477:1482	arg1	addition					1484:1491	site-specific glycan addition	1463:1491	site-specific glycan addition	1463:1491	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	12	70	gly	O-glycosylation	1574:1588	arg1	IgA1					1628:1631	IgA1	1628:1631	IgA1	1628:1631	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30759204	11	71	theme	IgA1	1348:1351	arg1	density					1363:1369	IgA1 HR glycan density	1348:1369	IgA1 HR glycan density	1348:1369	The lectin domain predominately enhanced IgA1 HR glycan density by increasing synthesis pathway exploration by GalNAc-T2.					
30759204	12	72	theme	glycoform	1603:1611	arg1	populations					1613:1623	glycoform populations	1603:1623	glycoform populations	1603:1623	Our data indicated a link between site-specific glycan addition and clustered glycan density that defines a mechanism of how conserved clustered O-glycosylation patterns and glycoform populations of IgA1 can be controlled by GalNAc-T2.					
30385505	0	0	theme	galectin-glycan	92:106	arg1	specificities					108:120	galectin-glycan specificities	92:120	galectin-glycan specificities	92:120	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	11	1	theme	Structural	1767:1776	arg1	modeling					1778:1785	Structural modeling	1767:1785	Structural modeling	1767:1785	Structural modeling provided a basis for interpreting the observed binding preferences.					
30385505	3	2	dep	such	607:610	arg1	as					612:613	as	612:613	as	612:613	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	9	3	theme	O-linked	1527:1534	arg1	glycans					1536:1542	O-linked glycans	1527:1542	O-linked glycans	1527:1542	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	6	4	theme	flow	1079:1082	arg1	cytometry					1084:1092	flow cytometry	1079:1092	flow cytometry	1079:1092	Using flow cytometry, we measured the cell-surface binding of the galectins.					
30385505	7	5	theme	glycoengineered	1307:1321	arg1	cells					1323:1327	the glycoengineered cells	1303:1327	the glycoengineered cells	1303:1327	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	9	6	link	O-linked	1527:1534	arg1	glycans					1536:1542	O-linked glycans	1527:1542	O-linked glycans	1527:1542	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	2	7	theme	β-galactoside-binding	390:410	arg1	site					412:415	their β-galactoside-binding site	384:415	their β-galactoside-binding site	384:415	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	8	8	theme	high-affinity	1471:1483	arg1	binding					1485:1491	high-affinity binding	1471:1491	high-affinity binding	1471:1491	We found that all galectins, apart from galectin-8N, require complex N-glycans for high-affinity binding.					
30385505	7	9	with	N-glycans	1346:1354	arg1	structures					1376:1385	defined branch structures	1361:1385	defined branch structures	1361:1385	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	2	10	from	sites	366:370	arg1	interactions					350:361	glycan interactions	343:361	glycan interactions at sites adjacent to their β-galactoside-binding site	343:415	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	3	11	theme	other	580:584	arg1	components					595:604	other cellular components	580:604	other cellular components	580:604	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	6	12	theme	cell-surface	1111:1122	arg1	binding					1124:1130	the cell-surface binding	1107:1130	the cell-surface binding of the galectins	1107:1147	Using flow cytometry, we measured the cell-surface binding of the galectins.					
30385505	3	13	theme	cellular	586:593	arg1	components					595:604	other cellular components	580:604	other cellular components	580:604	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	4	14	theme	cellular	793:800	arg1	context					802:808	the cellular context	789:808	the cellular context	789:808	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	0	15	gly	glycosylation	70:82	arg1	binding					9:15	Galectin binding	0:15	Galectin binding to cells and glycoproteins with genetically modified glycosylation	0:82	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	7	16	theme	synthetic	1336:1344	arg1	N-glycans					1346:1354	synthetic N-glycans	1336:1354	synthetic N-glycans with defined branch structures	1336:1385	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	2	17	theme	fine-tune	318:326	arg1	specificity					328:338	fine-tune specificity	318:338	fine-tune specificity	318:338	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	5	18	theme	different	978:986	arg1	domains					1013:1019	10 different carbohydrate-recognition domains	975:1019	10 different carbohydrate-recognition domains in galectins	975:1032	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	0	19	theme	natural	127:133	arg1	context					135:141	a natural context	125:141	a natural context	125:141	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	9	20	theme	LacNAc	1611:1616	arg1	structures					1619:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	9	21	theme	2,3-sialylated	1575:1588	arg1	structures					1619:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	7	22	gly	glycoprotein	1278:1289	arg1	glycoprotein					1278:1289	a reporter glycoprotein	1267:1289	a reporter glycoprotein produced by the glycoengineered cells	1267:1327	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	5	23	theme	defined	921:927	arg1	glycans					929:935	defined glycans	921:935	defined glycans	921:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	7	24	used	used	1163:1166	arg2	we					1160:1161	we	1160:1161	we	1160:1161	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	5	25	theme	binding	953:959	arg1	affinities					961:970	the binding affinities	949:970	the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans	949:1070	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	7	26	theme	fluorescence	1168:1179	arg1	anisotropy					1181:1190	fluorescence anisotropy	1168:1190	fluorescence anisotropy	1168:1190	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	5	27	theme	carbohydrate-recognition	988:1011	arg1	domains					1013:1019	10 different carbohydrate-recognition domains	975:1019	10 different carbohydrate-recognition domains in galectins	975:1032	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	2	28	theme	extensive	433:441	arg1	testing					443:449	extensive testing	433:449	extensive testing against panels of purified glycans	433:484	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	3	29	from	glycans	521:527	arg1	glycolipids					550:560	glycolipids	550:560	glycolipids	550:560	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	3	29	from	glycans	521:527	arg1	glycoproteins					532:544	glycoproteins	532:544	glycoproteins	532:544	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	3	30	gly	glycoproteins	532:544	arg1	glycoproteins					532:544	glycoproteins	532:544	glycoproteins	532:544	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	7	31	theme	branch	1369:1374	arg1	structures					1376:1385	defined branch structures	1361:1385	defined branch structures	1361:1385	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	1	32	theme	conserved	192:200	arg1	motif					211:215	a conserved sequence motif	190:215	a conserved sequence motif conferring affinity for β-galactose-containing glycans	190:270	Galectins compose a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans.					
30385505	0	33	theme	Galectin	0:7	arg1	binding					9:15	Galectin binding	0:15	Galectin binding to cells and glycoproteins with genetically modified glycosylation	0:82	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	5	34	from	affinities	961:970	arg1	galectins					1024:1032	galectins	1024:1032	galectins	1024:1032	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	5	35	theme	domains	1013:1019	arg1	affinities					961:970	the binding affinities	949:970	the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans	949:1070	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	8	36	theme	complex	1449:1455	arg1	N-glycans					1457:1465	complex N-glycans	1449:1465	complex N-glycans	1449:1465	We found that all galectins, apart from galectin-8N, require complex N-glycans for high-affinity binding.					
30385505	1	37	theme	sequence	202:209	arg1	motif					211:215	a conserved sequence motif	190:215	a conserved sequence motif conferring affinity for β-galactose-containing glycans	190:270	Galectins compose a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans.					
30385505	12	38	theme	glycoengineered	1895:1909	arg1	platform					1911:1918	a glycoengineered platform	1893:1918	a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins	1893:1995	These results underscore the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins.					
30385505	2	39	theme	glycan	343:348	arg1	interactions					350:361	glycan interactions	343:361	glycan interactions at sites adjacent to their β-galactoside-binding site	343:415	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	12	40	theme	proteins	1988:1995	arg1	specificities					1950:1962	the glycan-binding specificities	1931:1962	the glycan-binding specificities of carbohydrate-binding proteins	1931:1995	These results underscore the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins.					
30385505	2	41	theme	purified	469:476	arg1	glycans					478:484	purified glycans	469:484	purified glycans	469:484	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	2	42	theme	glycans	478:484	arg1	panels					459:464	panels	459:464	panels of purified glycans	459:484	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	5	43	used	used	841:844	arg2	we					838:839	we	838:839	we	838:839	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	4	44	theme	detailed	729:736	arg1	understanding					738:750	a detailed understanding	727:750	a detailed understanding of galectin-binding specificities	727:784	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	5	45	theme	mutated	890:896	arg1	cells					902:906	genetically stable glycosylation mutated CHO cells	857:906	genetically stable glycosylation mutated CHO cells that express defined glycans	857:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	12	46	theme	platform	1911:1918	arg1	power					1884:1888	the power	1880:1888	the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins	1880:1995	These results underscore the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins.					
30385505	5	47	theme	CHO	898:900	arg1	cells					902:906	genetically stable glycosylation mutated CHO cells	857:906	genetically stable glycosylation mutated CHO cells that express defined glycans	857:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	3	48	theme	cell	622:625	arg1	surface					627:633	the cell surface	618:633	the cell surface	618:633	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	5	49	from	domains	1013:1019	arg1	galectins					1024:1032	galectins	1024:1032	galectins	1024:1032	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	4	50	theme	cellular	686:693	arg1	environments					695:706	natural cellular environments	678:706	natural cellular environments	678:706	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	6	51	theme	galectins	1139:1147	arg1	binding					1124:1130	the cell-surface binding	1107:1130	the cell-surface binding of the galectins	1107:1147	Using flow cytometry, we measured the cell-surface binding of the galectins.					
30385505	7	52	theme	recombinant	1232:1242	arg1	erythropoietin					1244:1257	recombinant erythropoietin	1232:1257	recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells	1232:1327	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	4	53	theme	natural	678:684	arg1	environments					695:706	natural cellular environments	678:706	natural cellular environments	678:706	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	7	54	theme	galectin	1209:1216	arg1	affinities					1218:1227	the galectin affinities	1205:1227	the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures	1205:1385	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	9	55	theme	high	1549:1552	arg1	affinity					1554:1561	high affinity	1549:1561	high affinity	1549:1561	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	10	56	theme	galectins	1717:1725	arg1	binding					1699:1705	high-affinity binding	1685:1705	high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7	1685:1764	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	9	57	theme	N-acetyllactosamine	1590:1608	arg1	structures					1619:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	4	58	theme	galectin-binding	755:770	arg1	specificities					772:784	galectin-binding specificities	755:784	galectin-binding specificities	755:784	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	9	59	gly	2,3-sialylated	1575:1588	arg1	structures					1619:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	2,3-sialylated N-acetyllactosamine (LacNAc) structures	1575:1628	Galectin-8N targeted both N- and O-linked glycans with high affinity, preferring 2,3-sialylated N-acetyllactosamine (LacNAc) structures.					
30385505	0	60	with	binding	9:15	arg1	glycosylation					70:82	genetically modified glycosylation	49:82	genetically modified glycosylation	49:82	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	11	61	theme	observed	1825:1832	arg1	preferences					1842:1852	the observed binding preferences	1821:1852	the observed binding preferences	1821:1852	Structural modeling provided a basis for interpreting the observed binding preferences.					
30385505	2	62	theme	higher	298:303	arg1	affinity					305:312	higher affinity	298:312	higher affinity	298:312	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	12	63	theme	carbohydrate-binding	1967:1986	arg1	proteins					1988:1995	carbohydrate-binding proteins	1967:1995	carbohydrate-binding proteins	1967:1995	These results underscore the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins.					
30385505	2	64	theme	adjacent	372:379	arg1	sites					366:370	sites	366:370	sites adjacent to their β-galactoside-binding site	366:415	Moreover, galectins gain higher affinity and fine-tune specificity by glycan interactions at sites adjacent to their β-galactoside-binding site, as revealed by extensive testing against panels of purified glycans.					
30385505	3	65	theme	components	595:604	arg1	context					569:575	the context	565:575	the context of other cellular components	565:604	However, in cells, galectins bind glycans on glycoproteins and glycolipids in the context of other cellular components, such as at the cell surface.					
30385505	1	66	theme	β-galactose-containing	241:262	arg1	glycans					264:270	β-galactose-containing glycans	241:270	β-galactose-containing glycans	241:270	Galectins compose a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans.					
30385505	5	67	theme	mucin-type	1051:1060	arg1	O-glycans					1062:1070	mucin-type O-glycans	1051:1070	mucin-type O-glycans	1051:1070	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	11	68	theme	binding	1834:1840	arg1	preferences					1842:1852	the observed binding preferences	1821:1852	the observed binding preferences	1821:1852	Structural modeling provided a basis for interpreting the observed binding preferences.					
30385505	0	69	gly	glycoproteins	30:42	arg1	glycoproteins					30:42	glycoproteins	30:42	glycoproteins	30:42	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	4	70	theme	specificities	772:784	arg1	understanding					738:750	a detailed understanding	727:750	a detailed understanding of galectin-binding specificities	727:784	Because of difficulties in characterizing natural cellular environments, we currently lack a detailed understanding of galectin-binding specificities in the cellular context.					
30385505	7	71	theme	defined	1361:1367	arg1	structures					1376:1385	defined branch structures	1361:1385	defined branch structures	1361:1385	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	10	72	theme	high-affinity	1685:1697	arg1	binding					1699:1705	high-affinity binding	1685:1705	high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7	1685:1764	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	5	73	theme	cells	902:906	arg1	panel					848:852	a panel	846:852	a panel of genetically stable glycosylation mutated CHO cells that express defined glycans	846:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	5	74	theme	glycosylation	876:888	arg1	cells					902:906	genetically stable glycosylation mutated CHO cells	857:906	genetically stable glycosylation mutated CHO cells that express defined glycans	857:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	5	75	from	galectins	1024:1032	arg1	affinities					961:970	the binding affinities	949:970	the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans	949:1070	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	0	76	theme	modified	61:68	arg1	glycosylation					70:82	genetically modified glycosylation	49:82	genetically modified glycosylation	49:82	Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin-glycan specificities in a natural context.					
30385505	7	77	theme	reporter	1269:1276	arg1	glycoprotein					1278:1289	a reporter glycoprotein	1267:1289	a reporter glycoprotein produced by the glycoengineered cells	1267:1327	Moreover, we used fluorescence anisotropy to determine the galectin affinities to recombinant erythropoietin used as a reporter glycoprotein produced by the glycoengineered cells and to synthetic N-glycans with defined branch structures.					
30385505	5	78	theme	stable	869:874	arg1	cells					902:906	genetically stable glycosylation mutated CHO cells	857:906	genetically stable glycosylation mutated CHO cells that express defined glycans	857:935	To address this challenge, we used a panel of genetically stable glycosylation mutated CHO cells that express defined glycans to evaluate the binding affinities of 10 different carbohydrate-recognition domains in galectins to N-glycans and mucin-type O-glycans.					
30385505	12	79	theme	glycan-binding	1935:1948	arg1	specificities					1950:1962	the glycan-binding specificities	1931:1962	the glycan-binding specificities of carbohydrate-binding proteins	1931:1995	These results underscore the power of a glycoengineered platform to dissect the glycan-binding specificities of carbohydrate-binding proteins.					
30385505	1	80	theme	protein	164:170	arg1	family					172:177	a protein family	162:177	a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans	162:270	Galectins compose a protein family defined by a conserved sequence motif conferring affinity for β-galactose-containing glycans.					
30385505	10	81	theme	select	1710:1715	arg1	galectin-2					1738:1747	galectin-2	1738:1747	galectin-2	1738:1747	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	10	81	theme	select	1710:1715	arg1	-4N					1754:1756	-4N	1754:1756	-4N	1754:1756	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	10	81	theme	select	1710:1715	arg1	-7					1763:1764	-7	1763:1764	-7	1763:1764	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	10	81	theme	select	1710:1715	arg1	-3					1750:1751	-3	1750:1751	-3	1750:1751	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
30385505	10	81	theme	select	1710:1715	arg1	galectins					1717:1725	select galectins	1710:1725	select galectins	1710:1725	Furthermore, we found that 2,3-sialylation suppresses high-affinity binding of select galectins, including galectin-2, -3, -4N, and -7.					
31807707	1	0	link	N-linked	110:117	arg1	glycosylation					119:131	N-linked glycosylation	110:131	N-linked glycosylation	110:131	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	2	1	theme	limited	410:416	arg1	understanding					418:430	limited understanding	410:430	limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway	410:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	0	2	theme	processing	98:107	arg1	monitoring					61:70	monitoring	61:70	monitoring of intracellular N-glycan processing	61:107	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	3	3	theme	site-specific	571:583	arg1	N-glycans					585:593	site-specific N-glycans	571:593	site-specific N-glycans	571:593	We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells.					
31807707	4	4	theme	model-based	627:637	arg1	analysis					639:646	a model-based analysis	625:646	a model-based analysis of structure-specific turnover rates	625:683	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	1	5	gly	glycosylation	119:131	arg1	processes					256:264	multiple key biological processes	232:264	multiple key biological processes	232:264	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	4	6	theme	intracellular	722:734	arg1	processing					745:754	intracellular N-glycan processing	722:754	intracellular N-glycan processing	722:754	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	4	7	theme	structure-specific	651:668	arg1	rates					679:683	structure-specific turnover rates	651:683	structure-specific turnover rates	651:683	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	2	8	theme	N-glycans	373:381	arg1	biosynthesis					357:368	the stepwise biosynthesis	344:368	the stepwise biosynthesis of N-glycans	344:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	4	9	theme	secretory	773:781	arg1	pathway					783:789	the entire secretory pathway	762:789	the entire secretory pathway	762:789	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	5	10	theme	processing	947:956	arg1	machinery					958:966	the glycoprotein processing machinery	930:966	the glycoprotein processing machinery	930:966	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	4	11	theme	entire	766:771	arg1	pathway					783:789	the entire secretory pathway	762:789	the entire secretory pathway	762:789	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	2	12	theme	responsible	328:338	arg1	machinery					318:326	the intracellular machinery	300:326	the intracellular machinery responsible for the stepwise biosynthesis of N-glycans	300:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	1	13	theme	proteins	204:211	arg1	stability					191:199	the thermodynamic stability	173:199	the thermodynamic stability of proteins	173:211	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	2	14	theme	machinery	318:326	arg1	incomplete					392:401	incomplete	392:401	incomplete	392:401	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	2	14	theme	machinery	318:326	arg1	understanding					283:295	The mechanistic understanding	267:295	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans	267:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	2	15	theme	kinetics	443:450	arg1	understanding					418:430	limited understanding	410:430	limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway	410:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	4	16	theme	kinetic	699:705	arg1	description					707:717	a kinetic description	697:717	a kinetic description of intracellular N-glycan processing along the entire secretory pathway	697:789	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	5	17	gly	glycoprotein	934:945	arg1	glycoprotein					934:945	the glycoprotein processing machinery	930:966	the glycoprotein processing machinery	930:966	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	1	18	theme	N-linked	110:117	arg1	glycosylation					119:131	N-linked glycosylation	110:131	N-linked glycosylation	110:131	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	4	19	theme	processing	745:754	arg1	description					707:717	a kinetic description	697:717	a kinetic description of intracellular N-glycan processing along the entire secretory pathway	697:789	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	0	20	theme	Mechanistic	0:10	arg1	reconstruction					12:25	Mechanistic reconstruction	0:25	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.	0:108	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	2	21	theme	in	435:436	arg1	kinetics					443:450	in vivo kinetics	435:450	in vivo kinetics of N-glycan processing along the secretory pathway	435:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	0	22	theme	glycoprotein	30:41	arg1	secretion					43:51	glycoprotein secretion	30:51	glycoprotein secretion	30:51	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	3	23	from	processing	557:566	arg1	cells					602:606	CHO cells	598:606	CHO cells	598:606	We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells.					
31807707	3	24	theme	glycoproteomics	517:531	arg1	approach					533:540	a glycoproteomics approach	515:540	a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells	515:606	We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells.					
31807707	2	25	theme	secretory	485:493	arg1	pathway					495:501	the secretory pathway	481:501	the secretory pathway	481:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	3	26	theme	CHO	598:600	arg1	cells					602:606	CHO cells	598:606	CHO cells	598:606	We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells.					
31807707	2	27	theme	stepwise	348:355	arg1	biosynthesis					357:368	the stepwise biosynthesis	344:368	the stepwise biosynthesis of N-glycans	344:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	1	28	theme	multiple	232:239	arg1	processes					256:264	multiple key biological processes	232:264	multiple key biological processes	232:264	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	5	29	theme	N-glycans	859:867	arg1	biosynthesis					869:880	N-glycans biosynthesis	859:880	N-glycans biosynthesis	859:880	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	1	30	theme	key	241:243	arg1	processes					256:264	multiple key biological processes	232:264	multiple key biological processes	232:264	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	2	31	theme	mechanistic	271:281	arg1	incomplete					392:401	incomplete	392:401	incomplete	392:401	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	2	31	theme	mechanistic	271:281	arg1	understanding					283:295	The mechanistic understanding	267:295	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans	267:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	4	32	theme	N-glycan	736:743	arg1	processing					745:754	intracellular N-glycan processing	722:754	intracellular N-glycan processing	722:754	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	2	33	theme	processing	464:473	arg1	kinetics					443:450	in vivo kinetics	435:450	in vivo kinetics of N-glycan processing along the secretory pathway	435:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	0	34	theme	secretion	43:51	arg1	reconstruction					12:25	Mechanistic reconstruction	0:25	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.	0:108	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	4	35	dep	analysis	639:646	arg1	basis					616:620	basis	616:620	basis	616:620	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	4	35	dep	analysis	639:646	arg1	the					612:614	the	612:614	the	612:614	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	5	36	theme	glycoprotein	934:945	arg1	machinery					958:966	the glycoprotein processing machinery	930:966	the glycoprotein processing machinery	930:966	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	1	37	theme	biological	245:254	arg1	processes					256:264	multiple key biological processes	232:264	multiple key biological processes	232:264	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	5	38	theme	current	838:844	arg1	knowledge					846:854	the current knowledge	834:854	the current knowledge on N-glycans biosynthesis	834:880	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	1	39	theme	fundamental	141:151	arg1	role					153:156	a fundamental role	139:156	a fundamental role	139:156	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
31807707	5	40	from	knowledge	846:854	arg1	biosynthesis					869:880	N-glycans biosynthesis	859:880	N-glycans biosynthesis	859:880	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	0	41	gly	glycoprotein	30:41	arg1	glycoprotein					30:41	glycoprotein secretion	30:51	glycoprotein secretion	30:51	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	5	42	from	alterations	915:925	arg1	machinery					958:966	the glycoprotein processing machinery	930:966	the glycoprotein processing machinery	930:966	This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.					
31807707	2	43	theme	intracellular	304:316	arg1	machinery					318:326	the intracellular machinery	300:326	the intracellular machinery responsible for the stepwise biosynthesis of N-glycans	300:381	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	4	44	theme	rates	679:683	arg1	analysis					639:646	a model-based analysis	625:646	a model-based analysis of structure-specific turnover rates	625:683	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	2	45	dep	in	435:436	arg1	vivo					438:441	vivo	438:441	vivo	438:441	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	4	46	theme	turnover	670:677	arg1	rates					679:683	structure-specific turnover rates	651:683	structure-specific turnover rates	651:683	On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway.					
31807707	0	47	theme	N-glycan	89:96	arg1	processing					98:107	intracellular N-glycan processing	75:107	intracellular N-glycan processing	75:107	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	2	48	theme	N-glycan	455:462	arg1	processing					464:473	N-glycan processing	455:473	N-glycan processing along the secretory pathway	455:501	The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway.					
31807707	3	49	theme	N-glycans	585:593	arg1	processing					557:566	the processing	553:566	the processing of site-specific N-glycans in CHO cells	553:606	We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells.					
31807707	0	50	theme	intracellular	75:87	arg1	processing					98:107	intracellular N-glycan processing	75:107	intracellular N-glycan processing	75:107	Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.					
31807707	1	51	theme	thermodynamic	177:189	arg1	stability					191:199	the thermodynamic stability	173:199	the thermodynamic stability of proteins	173:211	N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes.					
30576150	8	0	gly	mannosylated	1479:1490	arg1	GlcNAc2-Man6					1499:1510	GlcNAc2-Man6	1499:1510	GlcNAc2-Man6	1499:1510	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	0	gly	mannosylated	1479:1490	arg1	PNO10					1492:1496	mannosylated PNO10	1479:1496	mannosylated PNO10 (GlcNAc2-Man6)	1479:1511	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	11	1	theme	domestic	2192:2199	arg1	animals					2201:2207	large domestic animals	2186:2207	large domestic animals	2186:2207	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	4	2	theme	milk	530:533	arg1	N-glycans					535:543	the milk N-glycans	526:543	the milk N-glycans of Meishan and Yorkshire sows	526:573	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	8	3	theme	PNO17	1528:1532	arg1	abundance					1466:1474	a higher ( P < 0.05) abundance	1445:1474	a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows	1445:1577	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	5	4	dep	identified	771:780	arg1	mannosylated					910:921	mannosylated	910:921	were high mannosylated	900:921	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	5	4	dep	identified	771:780	arg1	fucosylated					830:840	fucosylated	830:840	were fucosylated	825:840	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	10	5	theme	N-glycome	1923:1931	arg1	characteristics					1933:1947	N-glycome characteristics	1923:1947	N-glycome characteristics	1923:1947	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	0	6	with	Relation	59:66	arg1	Microbiome					87:96	Offspring Gut Microbiome	73:96	Offspring Gut Microbiome	73:96	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	6	7	theme	sow	1022:1024	arg1	milk					1026:1029	sow milk	1022:1029	sow milk	1022:1029	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	2	8	used	utilized	233:240	arg2	N-glycans					211:219	N-glycans	211:219	N-glycans	211:219	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	8	9	gly	sialylated	1517:1526	arg1	GlcNAc5-Man3-Gal-NeuAc					1535:1556	GlcNAc5-Man3-Gal-NeuAc	1535:1556	GlcNAc5-Man3-Gal-NeuAc	1535:1556	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	9	gly	sialylated	1517:1526	arg1	PNO17					1528:1532	sialylated PNO17	1517:1532	sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc)	1517:1557	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	9	10	theme	amylovorus	1778:1787	arg1	Lactobacillus					1764:1776	Lactobacillus amylovorus	1764:1787	Lactobacillus amylovorus	1764:1787	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	9	10	theme	amylovorus	1778:1787	arg1	OTU706					1755:1760	OTU706	1755:1760	OTU706	1755:1760	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	4	11	theme	spectrometry-based	614:631	arg1	method					641:646	a mass spectrometry-based glycome method	607:646	a mass spectrometry-based glycome method	607:646	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	7	12	theme	different	1228:1236	arg1	structures					1238:1247	different structures	1228:1247	different structures	1228:1247	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	10	13	theme	certain	1994:2000	arg1	microbes					2013:2020	certain piglet gut microbes	1994:2020	certain piglet gut microbes	1994:2020	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	7	14	located	found	1145:1149	arg1	PNOs					1194:1197	PNOs	1194:1197	PNOs	1194:1197	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	14	located	found	1145:1149	arg2	differences					1068:1078	No compositional differences	1051:1078	No compositional differences between the two breeds or between different lactation times	1051:1138	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	14	located	found	1145:1149	arg1	oligosaccharides					1176:1191	porcine milk N-linked oligosaccharides	1154:1191	porcine milk N-linked oligosaccharides (PNOs)	1154:1198	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	5	15	theme	sow	785:787	arg1	milk					789:792	sow milk	785:792	sow milk	785:792	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	8	16	theme	fucosylated	1304:1314	arg1	PNO3					1316:1319	fucosylated PNO3	1304:1319	fucosylated PNO3 (GlcNAc4-Man3-Fuc)	1304:1338	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	16	theme	fucosylated	1304:1314	arg1	GlcNAc4-Man3-Fuc					1322:1337	GlcNAc4-Man3-Fuc	1322:1337	GlcNAc4-Man3-Fuc	1322:1337	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	10	17	theme	gut	2009:2011	arg1	microbes					2013:2020	certain piglet gut microbes	1994:2020	certain piglet gut microbes	1994:2020	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	10	18	theme	milk	1867:1870	arg1	N-glycome					1872:1880	the milk N-glycome	1863:1880	the milk N-glycome of Meishan and Yorkshire sows	1863:1910	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	1	19	theme	important	116:124	arg1	source					126:131	an important source	113:131	an important source of milk oligosaccharides	113:156	N-Glycans are an important source of milk oligosaccharides.					
30576150	1	19	theme	important	116:124	arg1	N-Glycans					99:107	N-Glycans	99:107	N-Glycans	99:107	N-Glycans are an important source of milk oligosaccharides.					
30576150	3	20	theme	gut	300:302	arg1	regulators					312:321	gut microbe regulators	300:321	gut microbe regulators	300:321	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	6	21	theme	first	1039:1043	arg1	time					1045:1048	the first time	1035:1048	the first time	1035:1048	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	0	22	theme	Potential	49:57	arg1	Relation					59:66	the Potential Relation	45:66	the Potential Relation with Offspring Gut Microbiome	45:96	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	1	23	theme	milk	136:139	arg1	oligosaccharides					141:156	milk oligosaccharides	136:156	milk oligosaccharides	136:156	N-Glycans are an important source of milk oligosaccharides.					
30576150	5	24	gly	sialylated	866:875	arg1	%					845:845	41%	843:845	41% (9 out of 22)	843:859	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	3	25	theme	milk	275:278	arg1	N-glycans					280:288	milk N-glycans	275:288	milk N-glycans	275:288	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	7	26	theme	compositional	1054:1066	arg1	differences					1068:1078	No compositional differences	1051:1078	No compositional differences between the two breeds or between different lactation times	1051:1138	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	3	27	theme	suckling	326:333	arg1	animals					335:341	suckling animals	326:341	suckling animals	326:341	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	8	28	theme	sialylated	1344:1353	arg1	GlcNAc4-Man3-Gal2-NeuAc					1362:1384	GlcNAc4-Man3-Gal2-NeuAc	1362:1384	GlcNAc4-Man3-Gal2-NeuAc	1362:1384	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	28	theme	sialylated	1344:1353	arg1	PNO18					1355:1359	sialylated PNO18	1344:1359	sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc)	1344:1385	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	4	29	theme	gut	719:721	arg1	microbiota					723:732	offspring gut microbiota	709:732	offspring gut microbiota	709:732	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	0	30	theme	Gut	83:85	arg1	Microbiome					87:96	Offspring Gut Microbiome	73:96	Offspring Gut Microbiome	73:96	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	9	31	theme	gut	1639:1641	arg1	populations					1653:1663	milk N-glycans and offspring gut microbial populations	1610:1663	milk N-glycans and offspring gut microbial populations	1610:1663	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	3	32	theme	considerable	357:368	arg1	attention					370:378	considerable attention	357:378	considerable attention	357:378	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	4	33	theme	milk	690:693	arg1	N-glycans					695:703	milk N-glycans	690:703	milk N-glycans	690:703	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	3	34	theme	milk	394:397	arg1	N-glycans					399:407	sow milk N-glycans	390:407	sow milk N-glycans	390:407	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	0	35	theme	New	0:2	arg1	Insights					4:11	New Insights	0:11	New Insights into Porcine Milk N-Glycome	0:39	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	9	36	gly	mannosylated	1690:1701	arg1	GlcNAc2-Man9					1710:1721	GlcNAc2-Man9	1710:1721	GlcNAc2-Man9	1710:1721	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	9	36	gly	mannosylated	1690:1701	arg1	PNO21					1703:1707	mannosylated PNO21	1690:1707	mannosylated PNO21 (GlcNAc2-Man9)	1690:1722	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	7	37	theme	N-linked	1167:1174	arg1	PNOs					1194:1197	PNOs	1194:1197	PNOs	1194:1197	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	37	theme	N-linked	1167:1174	arg1	oligosaccharides					1176:1191	porcine milk N-linked oligosaccharides	1154:1191	porcine milk N-linked oligosaccharides (PNOs)	1154:1198	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	38	theme	porcine	1154:1160	arg1	PNOs					1194:1197	PNOs	1194:1197	PNOs	1194:1197	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	38	theme	porcine	1154:1160	arg1	oligosaccharides					1176:1191	porcine milk N-linked oligosaccharides	1154:1191	porcine milk N-linked oligosaccharides (PNOs)	1154:1198	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	11	39	theme	N-glycan	2124:2131	arg1	metabolism					2133:2142	milk N-glycan metabolism	2119:2142	milk N-glycan metabolism	2119:2142	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	11	40	from	involvement	2088:2098	arg1	metabolism					2133:2142	milk N-glycan metabolism	2119:2142	milk N-glycan metabolism	2119:2142	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	3	41	theme	potential	419:427	arg1	effects					429:435	their potential effects	413:435	their potential effects upon the piglet's gut microbes in vivo	413:474	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	5	42	theme	N-glycan	746:753	arg1	structures					755:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	0	43	theme	Milk	26:29	arg1	N-Glycome					31:39	Porcine Milk N-Glycome	18:39	Porcine Milk N-Glycome	18:39	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	6	44	with	N-glycan	927:934	arg1	structure					949:957	a NeuGc structure	941:957	a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc)	941:1002	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	8	45	theme	Meishan	1419:1425	arg1	sows					1427:1430	Meishan sows	1419:1430	Meishan sows	1419:1430	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	6	46	dep	structure	949:957	arg1	GlcNAc4-Man3-Gal2-Fuc-Neu5Gc					974:1001	GlcNAc4-Man3-Gal2-Fuc-Neu5Gc	974:1001	GlcNAc4-Man3-Gal2-Fuc-Neu5Gc	974:1001	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	6	46	dep	structure	949:957	arg1	PNO20					967:971	PNO20	967:971	PNO20	967:971	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	3	47	theme	gut	455:457	arg1	microbes					459:466	the piglet's gut microbes	442:466	the piglet's gut microbes	442:466	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	8	48	theme	mannosylated	1479:1490	arg1	GlcNAc2-Man6					1499:1510	GlcNAc2-Man6	1499:1510	GlcNAc2-Man6	1499:1510	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	48	theme	mannosylated	1479:1490	arg1	PNO10					1492:1496	mannosylated PNO10	1479:1496	mannosylated PNO10 (GlcNAc2-Man6)	1479:1511	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	7	49	theme	different	1114:1122	arg1	times					1134:1138	different lactation times	1114:1138	different lactation times	1114:1138	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	10	50	theme	sows	1907:1910	arg1	N-glycome					1872:1880	the milk N-glycome	1863:1880	the milk N-glycome of Meishan and Yorkshire sows	1863:1910	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	8	51	theme	higher	1447:1452	arg1	abundance					1466:1474	a higher ( P < 0.05) abundance	1445:1474	a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows	1445:1577	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	6	52	theme	NeuGc	943:947	arg1	structure					949:957	a NeuGc structure	941:957	a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc)	941:1002	An N-glycan with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for the first time.					
30576150	11	53	theme	large	2186:2190	arg1	animals					2201:2207	large domestic animals	2186:2207	large domestic animals	2186:2207	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	8	54	theme	P	1456:1456	arg1	<					1458:1458	P < 0.05	1456:1463	P < 0.05	1456:1463	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	9	55	theme	milk	1610:1613	arg1	N-glycans					1615:1623	milk N-glycans	1610:1623	milk N-glycans	1610:1623	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	5	56	dep	%	809:809	arg1	22					821:822	22	821:822	22	821:822	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	8	57	theme	sialylated	1517:1526	arg1	GlcNAc5-Man3-Gal-NeuAc					1535:1556	GlcNAc5-Man3-Gal-NeuAc	1535:1556	GlcNAc5-Man3-Gal-NeuAc	1535:1556	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	57	theme	sialylated	1517:1526	arg1	PNO17					1528:1532	sialylated PNO17	1517:1532	sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc)	1517:1557	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	11	58	theme	milk	2119:2122	arg1	metabolism					2133:2142	milk N-glycan metabolism	2119:2142	milk N-glycan metabolism	2119:2142	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	7	59	link	N-linked	1167:1174	arg1	PNOs					1194:1197	PNOs	1194:1197	PNOs	1194:1197	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	59	link	N-linked	1167:1174	arg1	oligosaccharides					1176:1191	porcine milk N-linked oligosaccharides	1154:1191	porcine milk N-linked oligosaccharides (PNOs)	1154:1198	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	11	60	theme	microbes	2107:2114	arg1	involvement					2088:2098	the involvement	2084:2098	the involvement of gut microbes in milk N-glycan metabolism, which is important to the health	2084:2176	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	11	61	theme	future	2062:2067	arg1	elucidation					2069:2079	future elucidation	2062:2079	future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans	2062:2218	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	10	62	theme	piglet	2002:2007	arg1	microbes					2013:2020	certain piglet gut microbes	1994:2020	certain piglet gut microbes	1994:2020	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	2	63	located	found	196:200	arg2	oligosaccharides					179:194	free oligosaccharides	174:194	free oligosaccharides found in milk	174:208	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	2	63	located	found	196:200	arg1	milk					205:208	milk	205:208	milk	205:208	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	8	64	theme	Yorkshire	1564:1572	arg1	sows					1574:1577	Yorkshire sows	1564:1577	Yorkshire sows	1564:1577	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	65	gly	fucosylated	1304:1314	arg1	PNO3					1316:1319	fucosylated PNO3	1304:1319	fucosylated PNO3 (GlcNAc4-Man3-Fuc)	1304:1338	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	65	gly	fucosylated	1304:1314	arg1	GlcNAc4-Man3-Fuc					1322:1337	GlcNAc4-Man3-Fuc	1322:1337	GlcNAc4-Man3-Fuc	1322:1337	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	5	66	gly	fucosylated	830:840	arg1	structures					755:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	5	66	gly	fucosylated	830:840	arg1	%					809:809	36%	807:809	36% (8 out of 22)	807:823	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	8	67	theme	relative	1281:1288	arg1	abundances					1290:1299	The relative abundances	1277:1299	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc)	1277:1385	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	7	68	theme	structures	1238:1247	arg1	abundances					1214:1223	the abundances	1210:1223	the abundances of different structures within this class	1210:1265	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	5	69	dep	%	884:884	arg1	22					896:897	22	896:897	22	896:897	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	11	70	from	metabolism	2133:2142	arg1	elucidation					2069:2079	future elucidation	2062:2079	future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans	2062:2218	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	9	71	theme	Bacteroides	1804:1814	arg1	OTU1380					1794:1800	OTU1380	1794:1800	OTU1380	1794:1800	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	9	71	theme	Bacteroides	1804:1814	arg1	uniformis					1816:1824	Bacteroides uniformis	1804:1824	Bacteroides uniformis	1804:1824	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	8	72	theme	PNO3	1316:1319	arg1	abundances					1290:1299	The relative abundances	1277:1299	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc)	1277:1385	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	10	73	theme	microbes	2013:2020	arg1	abundances					1980:1989	abundances	1980:1989	abundances of certain piglet gut microbes	1980:2020	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	2	74	theme	gut	245:247	arg1	microbes					249:256	gut microbes	245:256	gut microbes	245:256	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	8	75	gly	sialylated	1344:1353	arg1	GlcNAc4-Man3-Gal2-NeuAc					1362:1384	GlcNAc4-Man3-Gal2-NeuAc	1362:1384	GlcNAc4-Man3-Gal2-NeuAc	1362:1384	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	8	75	gly	sialylated	1344:1353	arg1	PNO18					1355:1359	sialylated PNO18	1344:1359	sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc)	1344:1385	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	9	76	theme	Apparent	1580:1587	arg1	correlations					1589:1600	Apparent correlations	1580:1600	Apparent correlations between milk N-glycans and offspring gut microbial populations	1580:1663	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	3	77	theme	microbe	304:310	arg1	regulators					312:321	gut microbe regulators	300:321	gut microbe regulators	300:321	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	4	78	theme	glycome	633:639	arg1	method					641:646	a mass spectrometry-based glycome method	607:646	a mass spectrometry-based glycome method	607:646	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	11	79	theme	gut	2103:2105	arg1	microbes					2107:2114	gut microbes	2103:2114	gut microbes	2103:2114	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	0	80	theme	Offspring	73:81	arg1	Microbiome					87:96	Offspring Gut Microbiome	73:96	Offspring Gut Microbiome	73:96	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	1	81	theme	oligosaccharides	141:156	arg1	source					126:131	an important source	113:131	an important source of milk oligosaccharides	113:156	N-Glycans are an important source of milk oligosaccharides.					
30576150	1	81	theme	oligosaccharides	141:156	arg1	N-Glycans					99:107	N-Glycans	99:107	N-Glycans	99:107	N-Glycans are an important source of milk oligosaccharides.					
30576150	9	82	theme	N-glycans	1615:1623	arg1	populations					1653:1663	milk N-glycans and offspring gut microbial populations	1610:1663	milk N-glycans and offspring gut microbial populations	1610:1663	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	8	83	theme	PNO18	1355:1359	arg1	abundances					1290:1299	The relative abundances	1277:1299	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc)	1277:1385	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	10	84	theme	Meishan	1885:1891	arg1	sows					1907:1910	Meishan and Yorkshire sows	1885:1910	Meishan and Yorkshire sows	1885:1910	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	9	85	theme	offspring	1629:1637	arg1	gut					1639:1641	offspring gut	1629:1641	offspring gut	1629:1641	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	4	86	theme	offspring	709:717	arg1	microbiota					723:732	offspring gut microbiota	709:732	offspring gut microbiota	709:732	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	5	87	dep	%	845:845	arg1	22					857:858	22	857:858	22	857:858	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	9	88	theme	microbial	1643:1651	arg1	populations					1653:1663	milk N-glycans and offspring gut microbial populations	1610:1663	milk N-glycans and offspring gut microbial populations	1610:1663	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	4	89	theme	Meishan	548:554	arg1	sows					570:573	Meishan and Yorkshire sows	548:573	Meishan and Yorkshire sows	548:573	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	8	90	dep	higher	1447:1452	arg1	<					1458:1458	P < 0.05	1456:1463	P < 0.05	1456:1463	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	7	91	theme	milk	1162:1165	arg1	PNOs					1194:1197	PNOs	1194:1197	PNOs	1194:1197	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	7	91	theme	milk	1162:1165	arg1	oligosaccharides					1176:1191	porcine milk N-linked oligosaccharides	1154:1191	porcine milk N-linked oligosaccharides (PNOs)	1154:1198	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	0	92	theme	Porcine	18:24	arg1	N-Glycome					31:39	Porcine Milk N-Glycome	18:39	Porcine Milk N-Glycome	18:39	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	2	93	theme	free	174:177	arg1	oligosaccharides					179:194	free oligosaccharides	174:194	free oligosaccharides found in milk	174:208	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	3	94	theme	sow	390:392	arg1	N-glycans					399:407	sow milk N-glycans	390:407	sow milk N-glycans	390:407	A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet's gut microbes in vivo remain unknown.					
30576150	11	95	theme	involvement	2088:2098	arg1	elucidation					2069:2079	future elucidation	2062:2079	future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans	2062:2218	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	7	96	theme	lactation	1124:1132	arg1	times					1134:1138	different lactation times	1114:1138	different lactation times	1114:1138	No compositional differences between the two breeds or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances of different structures within this class did vary.					
30576150	9	97	theme	mannosylated	1690:1701	arg1	GlcNAc2-Man9					1710:1721	GlcNAc2-Man9	1710:1721	GlcNAc2-Man9	1710:1721	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	9	97	theme	mannosylated	1690:1701	arg1	PNO21					1703:1707	mannosylated PNO21	1690:1707	mannosylated PNO21 (GlcNAc2-Man9)	1690:1722	Apparent correlations between milk N-glycans and offspring gut microbial populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 ( Lactobacillus amylovorus) and OTU1380 ( Bacteroides uniformis).					
30576150	4	98	theme	present	499:505	arg1	study					507:511	the present study	495:511	the present study	495:511	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	11	99	dep	involvement	2088:2098	arg1	both					2178:2181	both	2178:2181	both	2178:2181	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	0	100	with	Insights	4:11	arg1	Microbiome					87:96	Offspring Gut Microbiome	73:96	Offspring Gut Microbiome	73:96	New Insights into Porcine Milk N-Glycome and the Potential Relation with Offspring Gut Microbiome.					
30576150	8	101	theme	PNO10	1492:1496	arg1	abundance					1466:1474	a higher ( P < 0.05) abundance	1445:1474	a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows	1445:1577	The relative abundances of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased during lactation, and Meishan sows demonstrated a higher ( P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows.					
30576150	5	102	gly	mannosylated	910:921	arg1	structures					755:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures	735:764	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	5	102	gly	mannosylated	910:921	arg1	%					884:884	14%	882:884	14% (3 out of 22)	882:898	Twenty-two N-glycan structures were identified in sow milk, among which 36% (8 out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and 14% (3 out of 22) were high mannosylated.					
30576150	2	103	dep	oligosaccharides	179:194	arg1	addition					162:169	addition	162:169	addition	162:169	In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes.					
30576150	11	104	from	elucidation	2069:2079	arg1	metabolism					2133:2142	milk N-glycan metabolism	2119:2142	milk N-glycan metabolism	2119:2142	These findings provide a reference for future elucidation of the involvement of gut microbes in milk N-glycan metabolism, which is important to the health both of large domestic animals and humans.					
30576150	10	105	theme	Yorkshire	1897:1905	arg1	sows					1907:1910	Meishan and Yorkshire sows	1885:1910	Meishan and Yorkshire sows	1885:1910	Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to abundances of certain piglet gut microbes.					
30576150	4	106	theme	sows	570:573	arg1	N-glycans					535:543	the milk N-glycans	526:543	the milk N-glycans of Meishan and Yorkshire sows	526:573	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
30576150	4	107	theme	Yorkshire	560:568	arg1	sows					570:573	Meishan and Yorkshire sows	548:573	Meishan and Yorkshire sows	548:573	In the present study, we profiled the milk N-glycans of Meishan and Yorkshire sows during lactation using UPLC and a mass spectrometry-based glycome method, and we explored the correlations between milk N-glycans and offspring gut microbiota.					
31244828	7	0	theme	mutants	1198:1204	arg1	recognition					1151:1161	recognition	1151:1161	recognition of the two glycosylation-deficient mutants by different patients	1151:1226	We noted seven different patterns of recognition of the two glycosylation-deficient mutants by different patients.					
31244828	1	1	theme	MOG-transfected	281:295	arg1	cells					297:301	MOG-transfected cells	281:301	MOG-transfected cells	281:301	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	11	2	theme	HEK	1627:1629	arg1	cells					1631:1635	HEK cells	1627:1635	HEK cells	1627:1635	We determined the glycan structure of MOG produced in HEK cells by mass spectrometry.					
31244828	14	3	theme	glycosylation-deficient	2277:2299	arg1	mutant					2301:2306	a neutral glycosylation-deficient mutant	2267:2306	a neutral glycosylation-deficient mutant of MOG	2267:2313	Thus, a neutral glycosylation-deficient mutant of MOG might enhance the sensitivity to identify MOG-Abs.					
31244828	6	4	theme	mutations	1103:1111	arg1	mutations					1103:1111	the mutations	1099:1111	the mutations	1099:1111	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	6	4	theme	mutations	1103:1111	arg1	one					1084:1086	one	1084:1086	one	1084:1086	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	13	5	dep	reduces	2199:2205	arg1	in					2151:2152	in	2151:2152	in	2151:2152	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	5	dep	reduces	2199:2205	arg1	4					2148:2148	4	2148:2148	4	2148:2148	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	2	6	theme	mAb	333:335	arg1	8-18C5					337:342	the prototype anti-MOG mAb 8-18C5	310:342	the prototype anti-MOG mAb 8-18C5	310:342	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	12	7	theme	HEK	1708:1710	arg1	cells					1712:1716	HEK cells	1708:1716	HEK cells	1708:1716	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	10	8	theme	circular	1553:1560	arg1	dichroism					1562:1570	circular dichroism	1553:1570	circular dichroism	1553:1570	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	2	9	theme	prototype	314:322	arg1	8-18C5					337:342	the prototype anti-MOG mAb 8-18C5	310:342	the prototype anti-MOG mAb 8-18C5	310:342	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	5	10	theme	wild	840:843	arg1	MOG					850:852	wild type MOG	840:852	wild type MOG	840:852	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	8	11	theme	negative	1244:1251	arg1	charge					1253:1258	The introduced negative charge	1229:1258	The introduced negative charge at N31	1229:1265	The introduced negative charge at N31 enhanced recognition in some, but reduced recognition in other patients.					
31244828	4	12	gly	N-glycosylation	734:748	arg2	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	4	12	gly	N-glycosylation	734:748	arg2	one					724:726	one	724:726	one	724:726	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	4	12	gly	N-glycosylation	734:748	arg2	N31					758:760	N31	758:760	N31	758:760	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	3	13	theme	further	606:612	arg1	insight					614:620	further insight	606:620	further insight into details of antigen-recognition	606:656	The aim of this study was to get further insight into details of antigen-recognition by human MOG-Abs focusing on the impact of glycosylation.					
31244828	1	14	theme	patients	218:225	arg1	fraction					206:213	a fraction	204:213	a fraction of patients with inflammatory demyelination	204:257	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	6	15	theme	altered	1062:1068	arg1	reactivity					1070:1079	an altered reactivity	1059:1079	an altered reactivity to one or both of the mutations	1059:1111	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	11	16	theme	MOG	1611:1613	arg1	structure					1598:1606	the glycan structure	1587:1606	the glycan structure of MOG produced in HEK cells by mass spectrometry	1587:1656	We determined the glycan structure of MOG produced in HEK cells by mass spectrometry.					
31244828	5	17	theme	neutral	906:912	arg1	asparagine					914:923	the neutral asparagine	902:923	the neutral asparagine of N31	902:930	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	4	18	theme	N-glycosylation	734:748	arg1	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	1	19	theme	myelin	148:153	arg1	glycoprotein					172:183	myelin oligodendrocytes glycoprotein	148:183	myelin oligodendrocytes glycoprotein (MOG)	148:189	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	19	theme	myelin	148:153	arg1	MOG					186:188	MOG	186:188	MOG	186:188	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	12	20	theme	MOG	1691:1693	arg1	glycoforms					1677:1686	The most abundant glycoforms	1659:1686	The most abundant glycoforms of MOG expressed in HEK cells	1659:1716	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	20	theme	MOG	1691:1693	arg1	diantennary					1722:1732	diantennary	1722:1732	diantennary	1722:1732	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	21	from	MOG	1857:1859	arg1	myelin					1864:1869	myelin	1864:1869	myelin	1864:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	2	22	theme	different	525:533	arg1	epitopes					535:542	different epitopes	525:542	different epitopes	525:542	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	7	23	theme	glycosylation-deficient	1174:1196	arg1	mutants					1198:1204	the two glycosylation-deficient mutants	1166:1204	the two glycosylation-deficient mutants	1166:1204	We noted seven different patterns of recognition of the two glycosylation-deficient mutants by different patients.					
31244828	12	24	from	glycoforms	1843:1852	arg1	myelin					1864:1869	myelin	1864:1869	myelin	1864:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	25	theme	glycoforms	1843:1852	arg1	details					1828:1834	details	1828:1834	details of the glycoforms of MOG in myelin	1828:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	4	26	from	loop	780:783	arg1	located					762:768	located	762:768	located	762:768	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	13	27	theme	protein	2123:2129	arg1	backbone					2131:2138	the unglycosylated protein backbone	2104:2138	the unglycosylated protein backbone	2104:2138	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	0	28	theme	Large	100:104	arg1	Proportion					106:115	a Large Proportion	98:115	a Large Proportion of Patients	98:127	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	12	29	theme	MOG	1857:1859	arg1	glycoforms					1843:1852	the glycoforms	1839:1852	the glycoforms of MOG in myelin	1839:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	5	30	theme	charged	958:964	arg1	aspartate					966:974	negatively charged aspartate	947:974	negatively charged aspartate	947:974	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	10	31	theme	domain	1458:1463	arg1	folding					1429:1435	The folding	1425:1435	The folding of the extracellular domain of MOG with the formation of beta-sheets	1425:1504	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	2	32	theme	FG	429:430	arg1	loop					432:435	the FG loop	425:435	the FG loop of MOG	425:442	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	13	33	theme	human	1959:1963	arg1	autoantibodies					1965:1978	human autoantibodies	1959:1978	human autoantibodies to MOG	1959:1985	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	4	34	from	located	762:768	arg1	loop					780:783	the BC loop	773:783	the BC loop linking two beta-sheets	773:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	10	35	with	folding	1429:1435	arg1	formation					1481:1489	the formation	1477:1489	the formation of beta-sheets	1477:1504	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	13	36	gly	unglycosylated	2108:2121	arg1	backbone					2131:2138	the unglycosylated protein backbone	2104:2138	the unglycosylated protein backbone	2104:2138	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	37	theme	steric	2243:2248	arg1	hindrance					2250:2258	steric hindrance	2243:2258	steric hindrance	2243:2258	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	2	38	theme	human	449:453	arg1	response					464:471	the human anti-MOG response	445:471	the human anti-MOG response	445:471	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	2	38	theme	human	449:453	arg1	heterogeneous					481:493	heterogeneous	481:493	heterogeneous	481:493	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	4	39	theme	located	762:768	arg1	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	1	40	with	patients	218:225	arg1	demyelination					245:257	inflammatory demyelination	232:257	inflammatory demyelination	232:257	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	41	theme	oligodendrocytes	155:170	arg1	glycoprotein					172:183	myelin oligodendrocytes glycoprotein	148:183	myelin oligodendrocytes glycoprotein (MOG)	148:189	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	41	theme	oligodendrocytes	155:170	arg1	MOG					186:188	MOG	186:188	MOG	186:188	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	10	42	theme	MOG	1468:1470	arg1	MOG					1468:1470	MOG	1468:1470	MOG	1468:1470	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	10	42	theme	MOG	1468:1470	arg1	domain					1458:1463	the extracellular domain	1440:1463	the extracellular domain of MOG	1440:1470	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	0	43	theme	Myelin	26:31	arg1	Glycoprotein					49:60	Myelin Oligodendrocyte Glycoprotein	26:60	Myelin Oligodendrocyte Glycoprotein	26:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	13	44	theme	autoantibody	2207:2218	arg1	binding					2220:2226	autoantibody binding	2207:2226	autoantibody binding	2207:2226	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	12	45	theme	antennary	1761:1769	arg1	fucose					1750:1755	a core fucose	1743:1755	a core fucose	1743:1755	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	45	theme	antennary	1761:1769	arg1	fucose					1771:1776	an antennary fucose	1758:1776	an antennary fucose	1758:1776	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	0	46	theme	Autoantibody	70:81	arg1	Recognition					83:93	Autoantibody Recognition	70:93	Autoantibody Recognition	70:93	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	4	47	contain	has	720:722	arg2	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	4	47	contain	has	720:722	arg1	MOG					716:718	MOG	716:718	MOG	716:718	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	0	48	theme	Glycoprotein	49:60	arg1	Site					18:21	The Glycosylation Site	0:21	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein	0:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	0	48	theme	Glycoprotein	49:60	arg1	Glycoprotein					49:60	Myelin Oligodendrocyte Glycoprotein	26:60	Myelin Oligodendrocyte Glycoprotein	26:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	2	49	theme	polyclonal	348:357	arg1	responses					368:376	polyclonal anti-MOG responses	348:376	polyclonal anti-MOG responses from different mouse strains	348:405	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	9	50	theme	7/27	1343:1346	arg1	patients					1348:1355	7/27 patients	1343:1355	7/27 patients	1343:1355	In 7/27 patients the neutral glycosylation-deficient mutant was recognized stronger.					
31244828	12	51	theme	core	1745:1748	arg1	fucose					1750:1755	a core fucose	1743:1755	a core fucose	1743:1755	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	51	theme	core	1745:1748	arg1	fucose					1771:1776	an antennary fucose	1758:1776	an antennary fucose	1758:1776	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	2	52	theme	different	383:391	arg1	strains					399:405	different mouse strains	383:405	different mouse strains	383:405	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	9	53	theme	glycosylation-deficient	1369:1391	arg1	mutant					1393:1398	the neutral glycosylation-deficient mutant	1357:1398	the neutral glycosylation-deficient mutant	1357:1398	In 7/27 patients the neutral glycosylation-deficient mutant was recognized stronger.					
31244828	3	54	theme	glycosylation	701:713	arg1	impact					691:696	the impact	687:696	the impact of glycosylation	687:713	The aim of this study was to get further insight into details of antigen-recognition by human MOG-Abs focusing on the impact of glycosylation.					
31244828	14	55	theme	MOG	2311:2313	arg1	mutant					2301:2306	a neutral glycosylation-deficient mutant	2267:2306	a neutral glycosylation-deficient mutant of MOG	2267:2313	Thus, a neutral glycosylation-deficient mutant of MOG might enhance the sensitivity to identify MOG-Abs.					
31244828	11	56	theme	mass	1640:1643	arg1	spectrometry					1645:1656	mass spectrometry	1640:1656	mass spectrometry	1640:1656	We determined the glycan structure of MOG produced in HEK cells by mass spectrometry.					
31244828	2	57	from	strains	399:405	arg1	responses					368:376	polyclonal anti-MOG responses	348:376	polyclonal anti-MOG responses from different mouse strains	348:405	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	2	57	from	strains	399:405	arg1	8-18C5					337:342	the prototype anti-MOG mAb 8-18C5	310:342	the prototype anti-MOG mAb 8-18C5	310:342	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	13	58	dep	increase	1913:1920	arg1	1					1910:1910	1	1910:1910	1	1910:1910	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	58	dep	increase	1913:1920	arg1	show					1992:1995	show	1992:1995	show that the BC loop affects recognition in about 60% of the patients	1992:2061	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	58	dep	increase	1913:1920	arg1	2					1989:1989	2	1989:1989	2	1989:1989	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	2	59	theme	anti-MOG	324:331	arg1	8-18C5					337:342	the prototype anti-MOG mAb 8-18C5	310:342	the prototype anti-MOG mAb 8-18C5	310:342	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	3	60	theme	study	589:593	arg1	aim					577:579	The aim	573:579	The aim of this study	573:593	The aim of this study was to get further insight into details of antigen-recognition by human MOG-Abs focusing on the impact of glycosylation.					
31244828	13	61	theme	BC	2006:2007	arg1	loop					2009:2012	the BC loop	2002:2012	the BC loop	2002:2012	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	5	62	theme	type	845:848	arg1	MOG					850:852	wild type MOG	840:852	wild type MOG	840:852	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	8	63	theme	introduced	1233:1242	arg1	charge					1253:1258	The introduced negative charge	1229:1258	The introduced negative charge at N31	1229:1265	The introduced negative charge at N31 enhanced recognition in some, but reduced recognition in other patients.					
31244828	1	64	gly	glycoprotein	172:183	arg1	glycoprotein					172:183	myelin oligodendrocytes glycoprotein	148:183	myelin oligodendrocytes glycoprotein (MOG)	148:189	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	64	gly	glycoprotein	172:183	arg1	MOG					186:188	MOG	186:188	MOG	186:188	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	65	located	found	195:199	arg1	fraction					206:213	a fraction	204:213	a fraction of patients with inflammatory demyelination	204:257	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	1	65	located	found	195:199	arg2	Autoantibodies					130:143	Autoantibodies	130:143	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG)	130:189	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	12	66	gly	glycoforms	1843:1852	arg1	MOG					1857:1859	MOG	1857:1859	MOG in myelin	1857:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	67	theme	abundant	1668:1675	arg1	glycoforms					1677:1686	The most abundant glycoforms	1659:1686	The most abundant glycoforms of MOG expressed in HEK cells	1659:1716	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	67	theme	abundant	1668:1675	arg1	diantennary					1722:1732	diantennary	1722:1732	diantennary	1722:1732	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	4	68	theme	known	728:732	arg1	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	5	69	theme	different	875:883	arg1	mutants					885:891	two different mutants	871:891	two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine	871:1000	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	13	70	gly	heterogeneity	1942:1954	arg1	autoantibodies					1965:1978	human autoantibodies	1959:1978	human autoantibodies to MOG	1959:1985	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	7	71	theme	recognition	1151:1161	arg1	patterns					1139:1146	seven different patterns	1123:1146	seven different patterns of recognition of the two glycosylation-deficient mutants by different patients	1123:1226	We noted seven different patterns of recognition of the two glycosylation-deficient mutants by different patients.					
31244828	13	72	theme	unglycosylated	2108:2121	arg1	backbone					2131:2138	the unglycosylated protein backbone	2104:2138	the unglycosylated protein backbone	2104:2138	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	2	73	theme	human	499:503	arg1	MOG-Abs					505:511	human MOG-Abs	499:511	human MOG-Abs recognizing different epitopes	499:542	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	13	74	dep	report	2068:2073	arg1	3					2065:2065	3	2065:2065	3	2065:2065	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	12	75	gly	glycoforms	1677:1686	arg1	MOG					1691:1693	MOG	1691:1693	MOG expressed in HEK cells	1691:1716	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	76	contain	contain	1735:1741	arg1	glycoforms					1677:1686	The most abundant glycoforms	1659:1686	The most abundant glycoforms of MOG expressed in HEK cells	1659:1716	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	76	contain	contain	1735:1741	arg2	fucose					1771:1776	an antennary fucose	1758:1776	an antennary fucose	1758:1776	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	76	contain	contain	1735:1741	arg1	diantennary					1722:1732	diantennary	1722:1732	diantennary	1722:1732	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	12	76	contain	contain	1735:1741	arg2	fucose					1750:1755	a core fucose	1743:1755	a core fucose	1743:1755	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	8	77	from	N31	1263:1265	arg1	charge					1253:1258	The introduced negative charge	1229:1258	The introduced negative charge at N31	1229:1265	The introduced negative charge at N31 enhanced recognition in some, but reduced recognition in other patients.					
31244828	0	78	theme	Patients	120:127	arg1	Proportion					106:115	a Large Proportion	98:115	a Large Proportion of Patients	98:127	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	10	79	theme	extracellular	1444:1456	arg1	MOG					1468:1470	MOG	1468:1470	MOG	1468:1470	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	10	79	theme	extracellular	1444:1456	arg1	domain					1458:1463	the extracellular domain	1440:1463	the extracellular domain of MOG	1440:1470	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	4	80	theme	BC	777:778	arg1	loop					780:783	the BC loop	773:783	the BC loop linking two beta-sheets	773:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	7	81	theme	different	1129:1137	arg1	patterns					1139:1146	seven different patterns	1123:1146	seven different patterns of recognition of the two glycosylation-deficient mutants by different patients	1123:1226	We noted seven different patterns of recognition of the two glycosylation-deficient mutants by different patients.					
31244828	0	82	theme	Glycosylation	4:16	arg1	Site					18:21	The Glycosylation Site	0:21	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein	0:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	0	82	theme	Glycosylation	4:16	arg1	Glycoprotein					49:60	Myelin Oligodendrocyte Glycoprotein	26:60	Myelin Oligodendrocyte Glycoprotein	26:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	13	83	theme	autoantibodies	1965:1978	arg1	heterogeneity					1942:1954	heterogeneity	1942:1954	heterogeneity of human autoantibodies to MOG	1942:1985	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	4	84	from	N31	758:760	arg1	site					750:753	one known N-glycosylation site	724:753	one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets	724:807	MOG has one known N-glycosylation site at N31 located in the BC loop linking two beta-sheets.					
31244828	5	85	theme	N31	928:930	arg1	asparagine					914:923	the neutral asparagine	902:923	the neutral asparagine of N31	902:930	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	6	86	theme	patients	1035:1042	arg1	%					1026:1026	around 60%	1017:1026	around 60% of all patients (16/27)	1017:1050	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	6	86	theme	patients	1035:1042	arg1	patients					1035:1042	all patients	1031:1042	all patients (16/27)	1031:1050	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	6	86	theme	patients	1035:1042	arg1	16/27					1045:1049	16/27	1045:1049	16/27	1045:1049	We found that around 60% of all patients (16/27) showed an altered reactivity to one or both of the mutations.					
31244828	2	87	theme	anti-MOG	455:462	arg1	response					464:471	the human anti-MOG response	445:471	the human anti-MOG response	445:471	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	2	87	theme	anti-MOG	455:462	arg1	heterogeneous					481:493	heterogeneous	481:493	heterogeneous	481:493	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	8	88	theme	other	1324:1328	arg1	patients					1330:1337	other patients	1324:1337	other patients	1324:1337	The introduced negative charge at N31 enhanced recognition in some, but reduced recognition in other patients.					
31244828	13	89	theme	attached	2184:2191	arg1	sugar					2193:2197	the attached sugar	2180:2197	the attached sugar	2180:2197	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	12	90	theme	linked	1807:1812	arg1	Neu5Ac					1814:1819	linked Neu5Ac	1807:1819	linked Neu5Ac	1807:1819	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	3	91	theme	human	661:665	arg1	MOG-Abs					667:673	human MOG-Abs	661:673	human MOG-Abs focusing on the impact of glycosylation	661:713	The aim of this study was to get further insight into details of antigen-recognition by human MOG-Abs focusing on the impact of glycosylation.					
31244828	2	92	theme	MOG	440:442	arg1	loop					432:435	the FG loop	425:435	the FG loop of MOG	425:442	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	0	93	theme	Oligodendrocyte	33:47	arg1	Glycoprotein					49:60	Myelin Oligodendrocyte Glycoprotein	26:60	Myelin Oligodendrocyte Glycoprotein	26:60	The Glycosylation Site of Myelin Oligodendrocyte Glycoprotein Affects Autoantibody Recognition in a Large Proportion of Patients.					
31244828	3	94	theme	antigen-recognition	638:656	arg1	details					627:633	details	627:633	details of antigen-recognition	627:656	The aim of this study was to get further insight into details of antigen-recognition by human MOG-Abs focusing on the impact of glycosylation.					
31244828	14	95	theme	neutral	2269:2275	arg1	mutant					2301:2306	a neutral glycosylation-deficient mutant	2267:2306	a neutral glycosylation-deficient mutant of MOG	2267:2313	Thus, a neutral glycosylation-deficient mutant of MOG might enhance the sensitivity to identify MOG-Abs.					
31244828	10	96	theme	beta-sheets	1494:1504	arg1	formation					1481:1489	the formation	1477:1489	the formation of beta-sheets	1477:1504	The folding of the extracellular domain of MOG with the formation of beta-sheets did not depend on its glycosylation as seen by circular dichroism.					
31244828	2	97	theme	anti-MOG	359:366	arg1	responses					368:376	polyclonal anti-MOG responses	348:376	polyclonal anti-MOG responses from different mouse strains	348:405	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	13	98	theme	patients	2171:2178	arg1	%					2162:2162	20%	2160:2162	20% of the patients	2160:2178	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	98	theme	patients	2171:2178	arg1	patients					2171:2178	the patients	2167:2178	the patients	2167:2178	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	11	99	theme	glycan	1591:1596	arg1	structure					1598:1606	the glycan structure	1587:1606	the glycan structure of MOG produced in HEK cells by mass spectrometry	1587:1656	We determined the glycan structure of MOG produced in HEK cells by mass spectrometry.					
31244828	12	100	link	linked	1807:1812	arg1	Neu5Ac					1814:1819	linked Neu5Ac	1807:1819	linked Neu5Ac	1807:1819	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	1	101	theme	inflammatory	232:243	arg1	demyelination					245:257	inflammatory demyelination	232:257	inflammatory demyelination	232:257	Autoantibodies to myelin oligodendrocytes glycoprotein (MOG) are found in a fraction of patients with inflammatory demyelination and are detected with MOG-transfected cells.					
31244828	12	102	from	myelin	1864:1869	arg1	glycoforms					1843:1852	the glycoforms	1839:1852	the glycoforms of MOG in myelin	1839:1869	The most abundant glycoforms of MOG expressed in HEK cells are diantennary, contain a core fucose, an antennary fucose, and are decorated with α2,6 linked Neu5Ac, while details of the glycoforms of MOG in myelin remain to be identified.					
31244828	5	103	theme	neutral	986:992	arg1	alanine					994:1000	the neutral alanine	982:1000	the neutral alanine	982:1000	We compared the reactivity to wild type MOG with that toward two different mutants in which the neutral asparagine of N31 was mutated to negatively charged aspartate or to the neutral alanine.					
31244828	7	104	theme	different	1209:1217	arg1	patients					1219:1226	different patients	1209:1226	different patients	1209:1226	We noted seven different patterns of recognition of the two glycosylation-deficient mutants by different patients.					
31244828	13	105	theme	patients	2054:2061	arg1	%					2045:2045	about 60%	2037:2045	about 60% of the patients	2037:2061	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	13	105	theme	patients	2054:2061	arg1	patients					2054:2061	the patients	2050:2061	the patients	2050:2061	Together, we (1) increase the knowledge about heterogeneity of human autoantibodies to MOG, (2) show that the BC loop affects recognition in about 60% of the patients, (3) report that all patients recognized the unglycosylated protein backbone, while (4) in about 20% of the patients the attached sugar reduces autoantibody binding presumably via steric hindrance.					
31244828	2	106	theme	mouse	393:397	arg1	strains					399:405	different mouse strains	383:405	different mouse strains	383:405	While the prototype anti-MOG mAb 8-18C5 and polyclonal anti-MOG responses from different mouse strains largely recognize the FG loop of MOG, the human anti-MOG response is more heterogeneous and human MOG-Abs recognizing different epitopes were found to be pathogenic.					
31244828	9	107	theme	neutral	1361:1367	arg1	mutant					1393:1398	the neutral glycosylation-deficient mutant	1357:1398	the neutral glycosylation-deficient mutant	1357:1398	In 7/27 patients the neutral glycosylation-deficient mutant was recognized stronger.					
31384044	4	0	theme	glycopeptide	927:938	arg1	sequences					940:948	269 distinct glycopeptide sequences	914:948	269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins	914:1013	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	1	1	theme	spectrometry	159:170	arg1	strategy					190:197	tandem mass spectrometry O-glycoproteomics strategy	147:197	tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins	147:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	1	2	theme	O-glycoproteins	268:282	arg1	analysis					256:263	direct analysis	249:263	direct analysis of O-glycoproteins	249:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	0	3	theme	glycopeptide	78:89	arg1	libraries					91:99	silico-boosted glycopeptide libraries	63:99	silico-boosted glycopeptide libraries	63:99	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	4	4	theme	distinct	918:925	arg1	sequences					940:948	269 distinct glycopeptide sequences	914:948	269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins	914:1013	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	1	5	theme	O-glycoproteomics	172:188	arg1	strategy					190:197	tandem mass spectrometry O-glycoproteomics strategy	147:197	tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins	147:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	3	6	gly	O-glycoproteins	691:705	arg1	O-glycoproteins					691:705	2,076 O-glycoproteins	685:705	2,076 O-glycoproteins (11,452 unique glycopeptide sequences)	685:744	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	3	6	gly	O-glycoproteins	691:705	arg1	sequences					735:743	11,452 unique glycopeptide sequences	708:743	11,452 unique glycopeptide sequences	708:743	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	4	7	theme	core1	976:980	arg1	O-glycans					982:990	up to 5 different core1 O-glycans	958:990	up to 5 different core1 O-glycans	958:990	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	2	8	theme	glycopeptides	327:339	arg1	structures					345:354	structures	345:354	structures of O-glycans	345:367	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	2	8	theme	glycopeptides	327:339	arg1	characterization					307:322	characterization	307:322	characterization of glycopeptides	307:339	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	2	9	gly	glycopeptides	327:339	arg2	glycopeptides					327:339	glycopeptides	327:339	glycopeptides	327:339	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	3	10	theme	core1	768:772	arg1	structures					783:792	the 5 most common core1 O-glycan structures	750:792	the 5 most common core1 O-glycan structures	750:792	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	4	11	gly	glycoproteins	1001:1013	arg1	glycoproteins					1001:1013	159 glycoproteins	997:1013	159 glycoproteins	997:1013	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	4	12	theme	Glyco-DIA	808:816	arg1	library					818:824	the Glyco-DIA library	804:824	the Glyco-DIA library to human serum without enrichment	804:858	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	4	13	theme	SingleShot	1020:1029	arg1	analysis					1031:1038	a SingleShot analysis	1018:1038	a SingleShot analysis	1018:1038	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	0	14	from	O-glycoproteomics	37:53	arg1	libraries					91:99	silico-boosted glycopeptide libraries	63:99	silico-boosted glycopeptide libraries	63:99	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	0	15	gly	glycopeptide	78:89	arg2	glycopeptide					78:89	silico-boosted glycopeptide libraries	63:99	silico-boosted glycopeptide libraries	63:99	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	3	16	gly	glycopeptide	722:733	arg2	glycopeptide					722:733	11,452 unique glycopeptide sequences	708:743	11,452 unique glycopeptide sequences	708:743	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	3	17	theme	spectral	532:539	arg1	library					541:547	a spectral library	530:547	a spectral library of O-glycopeptides	530:566	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	4	18	gly	glycopeptide	927:938	arg2	glycopeptide					927:938	269 distinct glycopeptide sequences	914:948	269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins	914:1013	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	4	19	gly	glycopeptides	864:876	arg2	glycopeptides					864:876	glycopeptides	864:876	glycopeptides	864:876	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	3	20	theme	common	761:766	arg1	structures					783:792	the 5 most common core1 O-glycan structures	750:792	the 5 most common core1 O-glycan structures	750:792	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	1	21	gly	O-glycoproteins	268:282	arg1	O-glycoproteins					268:282	O-glycoproteins	268:282	O-glycoproteins	268:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	2	22	theme	O-glycans	359:367	arg1	structures					345:354	structures	345:354	structures of O-glycans	345:367	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	2	22	theme	O-glycans	359:367	arg1	characterization					307:322	characterization	307:322	characterization of glycopeptides	307:339	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	3	23	theme	Glyco-DIA	573:581	arg1	library					583:589	the Glyco-DIA library	569:589	the Glyco-DIA library	569:589	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	1	24	theme	data-independent	205:220	arg1	mode					240:243	data-independent acquisition (DIA) mode	205:243	data-independent acquisition (DIA) mode	205:243	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	3	25	theme	cell	633:636	arg1	lines					638:642	human cell lines	627:642	human cell lines	627:642	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	1	26	theme	acquisition	222:232	arg1	mode					240:243	data-independent acquisition (DIA) mode	205:243	data-independent acquisition (DIA) mode	205:243	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	2	27	theme	glycosite	482:490	arg1	identification					492:505	direct unambiguous glycosite identification	463:505	direct unambiguous glycosite identification	463:505	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	3	28	gly	O-glycopeptides	552:566	arg2	O-glycopeptides					552:566	O-glycopeptides	552:566	O-glycopeptides	552:566	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	2	29	theme	direct	463:468	arg1	identification					492:505	direct unambiguous glycosite identification	463:505	direct unambiguous glycosite identification	463:505	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	2	30	theme	unambiguous	470:480	arg1	identification					492:505	direct unambiguous glycosite identification	463:505	direct unambiguous glycosite identification	463:505	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	2	31	dep	stoichiometries	417:431	arg1	allow					453:457	allow	453:457	does not allow for direct unambiguous glycosite identification	444:505	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	3	32	theme	O-glycopeptides	552:566	arg1	library					541:547	a spectral library	530:547	a spectral library of O-glycopeptides	530:566	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	1	33	theme	DIA	235:237	arg1	mode					240:243	data-independent acquisition (DIA) mode	205:243	data-independent acquisition (DIA) mode	205:243	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	0	34	theme	quantitative	24:35	arg1	O-glycoproteomics					37:53	quantitative O-glycoproteomics	24:53	quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries	24:99	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	3	35	theme	human	648:652	arg1	serum					654:658	human serum	648:658	human serum	648:658	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	2	36	with	scale	388:392	arg1	quantification					399:412	quantification	399:412	quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification)	399:506	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	3	37	contain	contains	591:598	arg2	sublibraries					600:611	sublibraries	600:611	sublibraries obtained from human cell lines and human serum	600:658	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	3	37	contain	contains	591:598	arg1	library					583:589	the Glyco-DIA library	569:589	the Glyco-DIA library	569:589	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	4	38	theme	different	966:974	arg1	O-glycans					982:990	up to 5 different core1 O-glycans	958:990	up to 5 different core1 O-glycans	958:990	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	1	39	theme	liquid	114:119	arg1	chromatography					121:134	a liquid chromatography	112:134	a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins	112:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	3	40	theme	glycopeptide	722:733	arg1	O-glycoproteins					691:705	2,076 O-glycoproteins	685:705	2,076 O-glycoproteins (11,452 unique glycopeptide sequences)	685:744	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	3	40	theme	glycopeptide	722:733	arg1	sequences					735:743	11,452 unique glycopeptide sequences	708:743	11,452 unique glycopeptide sequences	708:743	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	2	41	theme	proteome-wide	374:386	arg1	scale					388:392	a proteome-wide scale	372:392	a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification)	372:506	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	0	42	dep	Glyco-DIA	0:8	arg1	method					13:18	a method	11:18	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.	0:100	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	3	43	theme	O-glycan	774:781	arg1	structures					783:792	the 5 most common core1 O-glycan structures	750:792	the 5 most common core1 O-glycan structures	750:792	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	1	44	theme	tandem	147:152	arg1	spectrometry					159:170	tandem mass spectrometry	147:170	tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins	147:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	3	45	theme	human	627:631	arg1	lines					638:642	human cell lines	627:642	human cell lines	627:642	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	4	46	theme	human	829:833	arg1	serum					835:839	human serum	829:839	human serum	829:839	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	1	47	theme	direct	249:254	arg1	analysis					256:263	direct analysis	249:263	direct analysis of O-glycoproteins	249:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
31384044	0	48	theme	silico-boosted	63:76	arg1	libraries					91:99	silico-boosted glycopeptide libraries	63:99	silico-boosted glycopeptide libraries	63:99	Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.					
31384044	3	49	theme	unique	715:720	arg1	O-glycoproteins					691:705	2,076 O-glycoproteins	685:705	2,076 O-glycoproteins (11,452 unique glycopeptide sequences)	685:744	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	3	49	theme	unique	715:720	arg1	sequences					735:743	11,452 unique glycopeptide sequences	708:743	11,452 unique glycopeptide sequences	708:743	The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures.					
31384044	2	50	theme	stoichiometries	417:431	arg1	quantification					399:412	quantification	399:412	quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification)	399:506	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	4	51	dep	5	964:964	arg1	to					961:962	to	961:962	to	961:962	Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.					
31384044	2	52	gly	glycosite	482:490	arg2	glycosite					482:490	direct unambiguous glycosite identification	463:505	direct unambiguous glycosite identification	463:505	This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification).					
31384044	1	53	theme	mass	154:157	arg1	spectrometry					159:170	tandem mass spectrometry	147:170	tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins	147:282	We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins.					
30295034	4	0	contain	contain	713:719	arg2	compositions					700:711	The dominant glycan compositions	680:711	The dominant glycan compositions	680:711	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	4	0	contain	contain	713:719	arg1	sites					777:781	all glycosylation sites	759:781	all glycosylation sites	759:781	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	4	0	contain	contain	713:719	arg2	structures					745:754	tri- and tetraantennary structures	721:754	tri- and tetraantennary structures	721:754	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	4	0	contain	contain	713:719	arg1	compositions					700:711	The dominant glycan compositions	680:711	The dominant glycan compositions	680:711	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	9	1	theme	individuals	1468:1478	arg1	samples					1450:1456	human plasma samples	1437:1456	human plasma samples of single individuals	1437:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	2	theme	structures	1592:1601	arg1	amount					1572:1577	the amount	1568:1577	the amount of antennary structures between individuals	1568:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	2	theme	structures	1592:1601	arg1	structures					1592:1601	antennary structures	1582:1601	antennary structures between individuals	1582:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	3	3	theme	numerous	580:587	arg1	species					596:602	numerous glycan species	580:602	numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa	580:677	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	1	4	theme	higher	288:293	arg1	structures					305:314	dominantly higher antennary structures	277:314	dominantly higher antennary structures	277:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	4	5	theme	dominant	684:691	arg1	compositions					700:711	The dominant glycan compositions	680:711	The dominant glycan compositions	680:711	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	0	6	theme	Intact	0:5	arg1	Glycoprotein					24:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	9	7	from	variations	1534:1543	arg1	fucosylation					1548:1559	fucosylation	1548:1559	fucosylation	1548:1559	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	7	from	variations	1534:1543	arg1	amount					1572:1577	the amount	1568:1577	the amount of antennary structures between individuals	1568:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	7	from	variations	1534:1543	arg1	structures					1592:1601	antennary structures	1582:1601	antennary structures between individuals	1582:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	1	8	theme	glycosylated	192:203	arg1	glycoprotein					161:172	Alpha-1-acid glycoprotein	148:172	Alpha-1-acid glycoprotein (AGP)	148:178	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	8	theme	glycosylated	192:203	arg1	protein					205:211	a highly glycosylated protein	183:211	a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures	183:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	2	9	gly	glycoprotein	537:548	arg1	glycoprotein					537:548	the intact glycoprotein	526:548	the intact glycoprotein	526:548	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	0	10	theme	Glycosylation	127:139	arg1	Sites					141:145	Multiple Glycosylation Sites	118:145	Multiple Glycosylation Sites	118:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	4	11	gly	glycosylation	763:775	arg2	sites					777:781	all glycosylation sites	759:781	all glycosylation sites	759:781	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	9	12	theme	desialylated	1496:1507	arg1	glycoproteins					1509:1521	desialylated glycoproteins	1496:1521	desialylated glycoproteins	1496:1521	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	12	theme	desialylated	1496:1507	arg1	variations					1534:1543	variations	1534:1543	variations in fucosylation and in the amount of antennary structures between individuals	1534:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	12	theme	desialylated	1496:1507	arg1	AGP					1428:1430	AGP	1428:1430	AGP from human plasma samples of single individuals	1428:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	5	13	gly	desialylated	927:938	arg1	AGP					940:942	the resulting desialylated AGP	913:942	the resulting desialylated AGP	913:942	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	1	14	gly	glycoprotein	161:172	arg1	AGP					175:177	AGP	175:177	AGP	175:177	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	14	gly	glycoprotein	161:172	arg1	protein					205:211	a highly glycosylated protein	183:211	a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures	183:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	14	gly	glycoprotein	161:172	arg1	glycoprotein					161:172	Alpha-1-acid glycoprotein	148:172	Alpha-1-acid glycoprotein (AGP)	148:178	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	5	15	theme	ionization-mass	960:974	arg1	spectrometry					976:987	electrospray ionization-mass spectrometry	947:987	electrospray ionization-mass spectrometry in positive ion mode	947:1008	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	4	16	theme	glycosylation	763:775	arg1	sites					777:781	all glycosylation sites	759:781	all glycosylation sites	759:781	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	1	17	theme	Alpha-1-acid	148:159	arg1	AGP					175:177	AGP	175:177	AGP	175:177	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	17	theme	Alpha-1-acid	148:159	arg1	protein					205:211	a highly glycosylated protein	183:211	a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures	183:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	17	theme	Alpha-1-acid	148:159	arg1	glycoprotein					161:172	Alpha-1-acid glycoprotein	148:172	Alpha-1-acid glycoprotein (AGP)	148:178	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	2	18	theme	glycoprotein	537:548	arg1	ESI-qTOF-MS					511:521	ESI-qTOF-MS	511:521	ESI-qTOF-MS of the intact glycoprotein in negative ion mode	511:569	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	4	19	theme	tri-	721:724	arg1	structures					745:754	tri- and tetraantennary structures	721:754	tri- and tetraantennary structures	721:754	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	0	20	theme	Simultaneous	62:73	arg1	Determination					75:87	Simultaneous Determination	62:87	Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites	62:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	3	21	theme	species	649:655	arg1	range					623:627	the mass range	614:627	the mass range of the glycoprotein species	614:655	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	5	22	theme	desialylated	927:938	arg1	AGP					940:942	the resulting desialylated AGP	913:942	the resulting desialylated AGP	913:942	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	9	23	gly	glycoproteins	1509:1521	arg1	glycoproteins					1509:1521	desialylated glycoproteins	1496:1521	desialylated glycoproteins	1496:1521	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	23	gly	glycoproteins	1509:1521	arg1	variations					1534:1543	variations	1534:1543	variations in fucosylation and in the amount of antennary structures between individuals	1534:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	23	gly	glycoproteins	1509:1521	arg1	AGP					1428:1430	AGP	1428:1430	AGP from human plasma samples of single individuals	1428:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	4	24	theme	tetraantennary	730:743	arg1	structures					745:754	tri- and tetraantennary structures	721:754	tri- and tetraantennary structures	721:754	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	8	25	with	antenae	1402:1408	arg1	acid					1422:1425	sialic acid	1415:1425	sialic acid	1415:1425	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	5	26	theme	fucosyl	811:817	arg1	units					819:823	two fucosyl units	807:823	two fucosyl units	807:823	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	0	27	theme	Glycan	96:101	arg1	Composition					103:113	the Glycan Composition	92:113	the Glycan Composition of Multiple Glycosylation Sites	92:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	1	28	theme	heterogeneity	388:400	arg1	characterization					361:376	characterization	361:376	characterization of glycan heterogeneity	361:400	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	29	gly	glycosylation	248:260	arg2	sites					262:266	five N-type glycosylation sites	236:266	five N-type glycosylation sites carrying dominantly higher antennary structures	236:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	29	gly	glycosylation	248:260	arg2	five					236:239	five	236:239	five	236:239	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	6	30	theme	oligosaccharides	1040:1055	arg1	pattern					1015:1021	The pattern	1011:1021	The pattern of nonsialylated oligosaccharides	1011:1055	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	2	31	theme	negative	553:560	arg1	mode					566:569	negative ion mode	553:569	negative ion mode	553:569	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	8	32	theme	complete	1374:1381	arg1	termination					1383:1393	an almost complete termination	1364:1393	an almost complete termination of all antenae with sialic acid	1364:1425	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	2	33	theme	glycan	422:427	arg1	composition					429:439	glycan composition	422:439	glycan composition over all five glycosylation sites	422:473	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	5	34	theme	mass	788:791	arg1	degeneracy					793:802	The mass degeneracy	784:802	The mass degeneracy of two fucosyl units versus one sialic acid	784:846	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	5	35	from	spectrometry	976:987	arg1	mode					1005:1008	positive ion mode	992:1008	positive ion mode	992:1008	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	9	36	theme	antennary	1582:1590	arg1	structures					1592:1601	antennary structures	1582:1601	antennary structures between individuals	1582:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	7	37	theme	genetic	1229:1235	arg1	ORM2					1267:1270	ORM2	1267:1270	ORM2	1267:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	37	theme	genetic	1229:1235	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	37	theme	genetic	1229:1235	arg1	F1					1251:1252	ORM1*F1	1246:1252	ORM1*F1	1246:1252	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	37	theme	genetic	1229:1235	arg1	S					1260:1260	ORM1*S	1255:1260	ORM1*S	1255:1260	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	1	38	theme	antennary	295:303	arg1	structures					305:314	dominantly higher antennary structures	277:314	dominantly higher antennary structures	277:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	5	39	theme	ion	1001:1003	arg1	mode					1005:1008	positive ion mode	992:1008	positive ion mode	992:1008	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	1	40	contain	carrying	268:275	arg2	structures					305:314	dominantly higher antennary structures	277:314	dominantly higher antennary structures	277:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	40	contain	carrying	268:275	arg1	sites					262:266	five N-type glycosylation sites	236:266	five N-type glycosylation sites carrying dominantly higher antennary structures	236:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	5	41	with	sample	877:882	arg1	sialidase					889:897	sialidase	889:897	sialidase	889:897	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	8	42	theme	antenae	1402:1408	arg1	termination					1383:1393	an almost complete termination	1364:1393	an almost complete termination of all antenae with sialic acid	1364:1425	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	0	43	theme	Alpha-Acid	13:22	arg1	Glycoprotein					24:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	6	44	gly	sialylated	1098:1107	arg1	species					1109:1115	the fully sialylated species	1088:1115	the fully sialylated species using bioinformatics tools	1088:1142	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	8	45	theme	sialic	1415:1420	arg1	acid					1422:1425	sialic acid	1415:1425	sialic acid	1415:1425	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	7	46	theme	different	1195:1203	arg1	compositions					1212:1223	90, 101, and 64 different glycan compositions	1179:1223	90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2	1179:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	6	47	theme	sialylated	1098:1107	arg1	species					1109:1115	the fully sialylated species	1088:1115	the fully sialylated species using bioinformatics tools	1088:1142	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	7	48	dep	variants	1237:1244	arg1	ORM2					1267:1270	ORM2	1267:1270	ORM2	1267:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	48	dep	variants	1237:1244	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	48	dep	variants	1237:1244	arg1	F1					1251:1252	ORM1*F1	1246:1252	ORM1*F1	1246:1252	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	48	dep	variants	1237:1244	arg1	S					1260:1260	ORM1*S	1255:1260	ORM1*S	1255:1260	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	5	49	theme	sialic	836:841	arg1	acid					843:846	one sialic acid	832:846	one sialic acid	832:846	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	1	50	theme	challenging	338:348	arg1	target					350:355	a challenging target	336:355	a challenging target for characterization of glycan heterogeneity	336:400	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	51	theme	N-type	241:246	arg1	sites					262:266	five N-type glycosylation sites	236:266	five N-type glycosylation sites carrying dominantly higher antennary structures	236:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	9	52	from	samples	1450:1456	arg1	glycoproteins					1509:1521	desialylated glycoproteins	1496:1521	desialylated glycoproteins	1496:1521	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	52	from	samples	1450:1456	arg1	variations					1534:1543	variations	1534:1543	variations in fucosylation and in the amount of antennary structures between individuals	1534:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	52	from	samples	1450:1456	arg1	AGP					1428:1430	AGP	1428:1430	AGP from human plasma samples of single individuals	1428:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	1	53	gly	glycosylated	192:203	arg1	glycoprotein					161:172	Alpha-1-acid glycoprotein	148:172	Alpha-1-acid glycoprotein (AGP)	148:178	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	53	gly	glycosylated	192:203	arg1	protein					205:211	a highly glycosylated protein	183:211	a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures	183:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	9	54	theme	human	1437:1441	arg1	samples					1450:1456	human plasma samples	1437:1456	human plasma samples of single individuals	1437:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	7	55	theme	ORM1	1255:1258	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	55	theme	ORM1	1255:1258	arg1	S					1260:1260	ORM1*S	1255:1260	ORM1*S	1255:1260	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	56	theme	human	1157:1161	arg1	plasma					1163:1168	pooled human plasma	1150:1168	pooled human plasma	1150:1168	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	6	57	gly	nonsialylated	1026:1038	arg1	oligosaccharides					1040:1055	nonsialylated oligosaccharides	1026:1055	nonsialylated oligosaccharides	1026:1055	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	9	58	theme	single	1461:1466	arg1	individuals					1468:1478	single individuals	1461:1478	single individuals	1461:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	6	59	theme	nonsialylated	1026:1038	arg1	oligosaccharides					1040:1055	nonsialylated oligosaccharides	1026:1055	nonsialylated oligosaccharides	1026:1055	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	3	60	gly	glycoprotein	636:647	arg1	glycoprotein					636:647	the glycoprotein species	632:655	the glycoprotein species	632:655	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	0	61	theme	Sites	141:145	arg1	Composition					103:113	the Glycan Composition	92:113	the Glycan Composition of Multiple Glycosylation Sites	92:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	3	62	theme	glycan	589:594	arg1	species					596:602	numerous glycan species	580:602	numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa	580:677	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	4	63	theme	glycan	693:698	arg1	compositions					700:711	The dominant glycan compositions	680:711	The dominant glycan compositions	680:711	The dominant glycan compositions contain tri- and tetraantennary structures on all glycosylation sites.					
30295034	1	64	theme	human	218:222	arg1	plasma					224:229	human plasma	218:229	human plasma	218:229	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	8	65	theme	Glycan	1287:1292	arg1	structures					1294:1303	Glycan structures	1287:1303	Glycan structures	1287:1303	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	7	66	theme	*	1250:1250	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	66	theme	*	1250:1250	arg1	F1					1251:1252	ORM1*F1	1246:1252	ORM1*F1	1246:1252	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	3	67	theme	mass	618:621	arg1	range					623:627	the mass range	614:627	the mass range of the glycoprotein species	614:655	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	1	68	from	plasma	224:229	arg1	glycoprotein					161:172	Alpha-1-acid glycoprotein	148:172	Alpha-1-acid glycoprotein (AGP)	148:178	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	1	68	from	plasma	224:229	arg1	protein					205:211	a highly glycosylated protein	183:211	a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures	183:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	5	69	theme	electrospray	947:958	arg1	spectrometry					976:987	electrospray ionization-mass spectrometry	947:987	electrospray ionization-mass spectrometry in positive ion mode	947:1008	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	9	70	gly	desialylated	1496:1507	arg1	glycoproteins					1509:1521	desialylated glycoproteins	1496:1521	desialylated glycoproteins	1496:1521	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	70	gly	desialylated	1496:1507	arg1	variations					1534:1543	variations	1534:1543	variations in fucosylation and in the amount of antennary structures between individuals	1534:1621	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	9	70	gly	desialylated	1496:1507	arg1	AGP					1428:1430	AGP	1428:1430	AGP from human plasma samples of single individuals	1428:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	7	71	theme	*	1259:1259	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	71	theme	*	1259:1259	arg1	S					1260:1260	ORM1*S	1255:1260	ORM1*S	1255:1260	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	3	72	theme	glycoprotein	636:647	arg1	species					649:655	the glycoprotein species	632:655	the glycoprotein species	632:655	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	5	73	theme	resulting	917:925	arg1	AGP					940:942	the resulting desialylated AGP	913:942	the resulting desialylated AGP	913:942	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	2	74	theme	intact	530:535	arg1	glycoprotein					537:548	the intact glycoprotein	526:548	the intact glycoprotein	526:548	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	7	75	theme	pooled	1150:1155	arg1	plasma					1163:1168	pooled human plasma	1150:1168	pooled human plasma	1150:1168	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	8	76	theme	sialic	1340:1345	arg1	acids					1347:1351	15 and 16 sialic acids	1330:1351	15 and 16 sialic acids	1330:1351	Glycan structures carry dominantly between 15 and 16 sialic acids indicating an almost complete termination of all antenae with sialic acid.					
30295034	0	77	theme	Composition	103:113	arg1	Determination					75:87	Simultaneous Determination	62:87	Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites	62:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	1	78	theme	glycan	381:386	arg1	heterogeneity					388:400	glycan heterogeneity	381:400	glycan heterogeneity	381:400	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	2	79	theme	ion	562:564	arg1	mode					566:569	negative ion mode	553:569	negative ion mode	553:569	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	0	80	theme	Multiple	118:125	arg1	Sites					141:145	Multiple Glycosylation Sites	118:145	Multiple Glycosylation Sites	118:145	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	9	81	theme	plasma	1443:1448	arg1	samples					1450:1456	human plasma samples	1437:1456	human plasma samples of single individuals	1437:1478	AGP from human plasma samples of single individuals was analyzed as desialylated glycoproteins and showed variations in fucosylation and in the amount of antennary structures between individuals.					
30295034	2	82	from	ESI-qTOF-MS	511:521	arg1	mode					566:569	negative ion mode	553:569	negative ion mode	553:569	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	0	83	theme	Human	7:11	arg1	Glycoprotein					24:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein	0:35	Intact Human Alpha-Acid Glycoprotein Analyzed by ESI-qTOF-MS: Simultaneous Determination of the Glycan Composition of Multiple Glycosylation Sites.					
30295034	5	84	theme	positive	992:999	arg1	mode					1005:1008	positive ion mode	992:1008	positive ion mode	992:1008	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	7	85	theme	glycan	1205:1210	arg1	compositions					1212:1223	90, 101, and 64 different glycan compositions	1179:1223	90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2	1179:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	6	86	theme	bioinformatics	1123:1136	arg1	tools					1138:1142	bioinformatics tools	1123:1142	bioinformatics tools	1123:1142	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	2	87	theme	glycosylation	455:467	arg1	sites					469:473	all five glycosylation sites	446:473	all five glycosylation sites	446:473	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	5	88	theme	units	819:823	arg1	degeneracy					793:802	The mass degeneracy	784:802	The mass degeneracy of two fucosyl units versus one sialic acid	784:846	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	1	89	with	protein	205:211	arg1	sites					262:266	five N-type glycosylation sites	236:266	five N-type glycosylation sites carrying dominantly higher antennary structures	236:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30295034	6	90	theme	species	1109:1115	arg1	interpretation					1070:1083	interpretation	1070:1083	interpretation of the fully sialylated species using bioinformatics tools	1070:1142	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	6	91	used	used	1061:1064	arg2	pattern					1015:1021	The pattern	1011:1021	The pattern of nonsialylated oligosaccharides	1011:1055	The pattern of nonsialylated oligosaccharides was used for interpretation of the fully sialylated species using bioinformatics tools.					
30295034	7	92	theme	ORM1	1246:1249	arg1	variants					1237:1244	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	genetic variants ORM1*F1, ORM1*S, and ORM2	1229:1270	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	7	92	theme	ORM1	1246:1249	arg1	F1					1251:1252	ORM1*F1	1246:1252	ORM1*F1	1246:1252	From pooled human plasma, we find 90, 101, and 64 different glycan compositions for genetic variants ORM1*F1, ORM1*S, and ORM2, respectively.					
30295034	3	93	dep	38.5	670:673	arg1	to					667:668	to	667:668	to	667:668	We find numerous glycan species extending the mass range of the glycoprotein species from 35.0 to 38.5 kDa.					
30295034	2	94	gly	glycosylation	455:467	arg2	five					450:453	five	450:453	five	450:453	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	2	94	gly	glycosylation	455:467	arg2	sites					469:473	all five glycosylation sites	446:473	all five glycosylation sites	446:473	Here, we show that glycan composition over all five glycosylation sites can be determined quantitatively by ESI-qTOF-MS of the intact glycoprotein in negative ion mode.					
30295034	5	95	theme	acid	843:846	arg1	degeneracy					793:802	The mass degeneracy	784:802	The mass degeneracy of two fucosyl units versus one sialic acid	784:846	The mass degeneracy of two fucosyl units versus one sialic acid was resolved by treating the sample with sialidase and analyzing the resulting desialylated AGP by electrospray ionization-mass spectrometry in positive ion mode.					
30295034	1	96	theme	glycosylation	248:260	arg1	sites					262:266	five N-type glycosylation sites	236:266	five N-type glycosylation sites carrying dominantly higher antennary structures	236:314	Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein from human plasma with five N-type glycosylation sites carrying dominantly higher antennary structures and thus represents a challenging target for characterization of glycan heterogeneity.					
30503285	0	0	theme	O-Linked	93:100	arg1	Glycans					102:108	Human-like O-Linked Glycans	82:108	Human-like O-Linked Glycans	82:108	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	4	1	theme	engineered	880:889	arg1	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	1	theme	engineered	880:889	arg1	site					891:894	one engineered site	876:894	one engineered site of glycosylation	876:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	2	with	hormone	863:869	arg1	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	2	with	hormone	863:869	arg1	site					891:894	one engineered site	876:894	one engineered site of glycosylation	876:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	3	with	α-2b	833:836	arg1	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	3	with	α-2b	833:836	arg1	site					891:894	one engineered site	876:894	one engineered site of glycosylation	876:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	0	4	theme	Human-like	82:91	arg1	Glycans					102:108	Human-like O-Linked Glycans	82:108	Human-like O-Linked Glycans	82:108	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	1	5	theme	in vivo	164:170	arg1	production					172:181	the in vivo production	160:181	the in vivo production of O-glycosylated proteins	160:208	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	4	6	theme	O-glycosylated	801:814	arg1	α-2b					833:836	the naturally O-glycosylated human interferon α-2b	787:836	the naturally O-glycosylated human interferon α-2b	787:836	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	5	7	theme	core-1	976:981	arg1	glycan					983:988	the core-1 glycan	972:988	the core-1 glycan onto proteins	972:1002	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	1	8	dep	Escherichia	132:142	arg1	coli					144:147	coli	144:147	coli	144:147	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	3	9	theme	human	406:410	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	9	theme	human	406:410	arg1	transferase					447:457	human polypeptide N-acetylgalactosaminyl transferase	406:457	human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin	406:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	1	10	gly	O-glycosylated	186:199	arg1	proteins					201:208	O-glycosylated proteins	186:208	O-glycosylated proteins	186:208	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	4	11	dep	proteins	710:717	arg1	native					751:756	native	751:756	native	751:756	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	11	dep	proteins	710:717	arg1	version					776:782	an engineered version	762:782	an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation	762:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	2	12	theme	protein	287:293	arg1	target					295:300	a therapeutic protein target	273:300	a therapeutic protein target	273:300	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	4	13	with	proteins	710:717	arg1	potential					736:744	therapeutic potential	724:744	therapeutic potential	724:744	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	14	theme	growth	856:861	arg1	hormone					863:869	human growth hormone	850:869	human growth hormone with one engineered site of glycosylation	850:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	2	15	theme	therapeutic	275:285	arg1	target					295:300	a therapeutic protein target	273:300	a therapeutic protein target	273:300	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	5	16	from	structures	1135:1144	arg1	coli					1152:1155	E. coli	1149:1155	E. coli	1149:1155	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	5	17	theme	established	921:931	arg1	proof					933:937	established proof	921:937	established proof of principle for the addition of the core-1 glycan onto proteins	921:1002	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	4	18	gly	O-glycosylated	801:814	arg1	α-2b					833:836	the naturally O-glycosylated human interferon α-2b	787:836	the naturally O-glycosylated human interferon α-2b	787:836	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	2	19	dep	approach	250:257	arg1	one					260:262	one	260:262	one	260:262	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	2	19	dep	approach	250:257	arg1	second					309:314	second	309:314	second	309:314	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	0	20	theme	Bacterial	2:10	arg1	Platform					23:30	A Bacterial Expression Platform	0:30	A Bacterial Expression Platform for Production of Therapeutic Proteins	0:69	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	5	21	theme	complex	1118:1124	arg1	structures					1135:1144	more complex O-glycan structures	1113:1144	more complex O-glycan structures in E. coli	1113:1155	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	1	22	theme	O-glycosylated	186:199	arg1	proteins					201:208	O-glycosylated proteins	186:208	O-glycosylated proteins	186:208	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	4	23	theme	hormone	863:869	arg1	version					776:782	an engineered version	762:782	an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation	762:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	1	24	theme	proteins	201:208	arg1	production					172:181	the in vivo production	160:181	the in vivo production of O-glycosylated proteins	160:208	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	3	25	theme	disulfide	568:576	arg1	isomerase					583:591	a disulfide bond isomerase	566:591	a disulfide bond isomerase	566:591	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	26	theme	human	609:613	arg1	origin					615:620	bacterial or human origin	596:620	bacterial or human origin	596:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	4	27	theme	two-plasmid	649:659	arg1	system					678:683	this two-plasmid synthetic operon system	644:683	this two-plasmid synthetic operon system	644:683	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	0	28	theme	Expression	12:21	arg1	Platform					23:30	A Bacterial Expression Platform	0:30	A Bacterial Expression Platform for Production of Therapeutic Proteins	0:69	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	3	29	theme	bond	578:581	arg1	isomerase					583:591	a disulfide bond isomerase	566:591	a disulfide bond isomerase	566:591	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	5	30	theme	glycan	983:988	arg1	addition					960:967	the addition	956:967	the addition of the core-1 glycan onto proteins	956:1002	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	3	31	theme	UDP-Glc	504:510	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	31	theme	UDP-Glc	504:510	arg1	-4-epimerase					516:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	32	theme	N-acetylgalactosaminyl	424:445	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	32	theme	N-acetylgalactosaminyl	424:445	arg1	transferase					447:457	human polypeptide N-acetylgalactosaminyl transferase	406:457	human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin	406:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	4	33	theme	therapeutic	724:734	arg1	potential					736:744	therapeutic potential	724:744	therapeutic potential	724:744	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	34	theme	operon	671:676	arg1	system					678:683	this two-plasmid synthetic operon system	644:683	this two-plasmid synthetic operon system	644:683	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	2	35	theme	plasmid	242:248	arg1	approach					250:257	a dual plasmid approach	235:257	a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation	235:376	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	3	36	theme	β1,3-galactosyl	472:486	arg1	transferase					488:498	β1,3-galactosyl transferase	472:498	β1,3-galactosyl transferase	472:498	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	36	theme	β1,3-galactosyl	472:486	arg1	NAc					512:514	NAc	512:514	NAc	512:514	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	5	37	theme	human	1081:1085	arg1	therapeutics					1095:1106	human protein therapeutics	1081:1106	human protein therapeutics	1081:1106	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	2	38	theme	dual	237:240	arg1	approach					250:257	a dual plasmid approach	235:257	a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation	235:376	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	3	39	theme	transferase	488:498	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	39	theme	transferase	488:498	arg1	-4-epimerase					516:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	0	40	theme	Therapeutic	50:60	arg1	Proteins					62:69	Therapeutic Proteins	50:69	Therapeutic Proteins	50:69	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	5	41	theme	protein	1087:1093	arg1	therapeutics					1095:1106	human protein therapeutics	1081:1106	human protein therapeutics	1081:1106	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	3	42	theme	bacterial	596:604	arg1	origin					615:620	bacterial or human origin	596:620	bacterial or human origin	596:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	43	theme	polypeptide	412:422	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	43	theme	polypeptide	412:422	arg1	transferase					447:457	human polypeptide N-acetylgalactosaminyl transferase	406:457	human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin	406:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	4	44	theme	glycosylation	899:911	arg1	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	44	theme	glycosylation	899:911	arg1	site					891:894	one engineered site	876:894	one engineered site of glycosylation	876:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	45	theme	human	850:854	arg1	hormone					863:869	human growth hormone	850:869	human growth hormone with one engineered site of glycosylation	850:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	2	46	theme	enzymatic	329:337	arg1	machinery					339:347	the enzymatic machinery	325:347	the enzymatic machinery required for O-glycosylation	325:376	This was achieved using a dual plasmid approach: one encoding a therapeutic protein target, and a second encoding the enzymatic machinery required for O-glycosylation.					
30503285	4	47	theme	interferon	822:831	arg1	α-2b					833:836	the naturally O-glycosylated human interferon α-2b	787:836	the naturally O-glycosylated human interferon α-2b	787:836	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	48	theme	human	816:820	arg1	α-2b					833:836	the naturally O-glycosylated human interferon α-2b	787:836	the naturally O-glycosylated human interferon α-2b	787:836	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	3	49	theme	origin	615:620	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	49	theme	origin	615:620	arg1	-4-epimerase					516:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	49	theme	origin	615:620	arg1	isomerase					583:591	a disulfide bond isomerase	566:591	a disulfide bond isomerase	566:591	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	49	theme	origin	615:620	arg1	transferase					447:457	human polypeptide N-acetylgalactosaminyl transferase	406:457	human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin	406:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	1	50	theme	Escherichia	132:142	arg1	strain					149:154	an Escherichia coli strain	129:154	an Escherichia coli strain for the in vivo production of O-glycosylated proteins	129:208	We have developed an Escherichia coli strain for the in vivo production of O-glycosylated proteins.					
30503285	4	51	theme	engineered	765:774	arg1	version					776:782	an engineered version	762:782	an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation	762:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	3	52	dep	transferase	488:498	arg1	a					470:470	a	470:470	a	470:470	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	5	53	theme	principle	942:950	arg1	proof					933:937	established proof	921:937	established proof of principle for the addition of the core-1 glycan onto proteins	921:1002	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	4	54	theme	synthetic	661:669	arg1	system					678:683	this two-plasmid synthetic operon system	644:683	this two-plasmid synthetic operon system	644:683	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	55	theme	α-2b	833:836	arg1	version					776:782	an engineered version	762:782	an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation	762:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	56	gly	glycosylation	899:911	arg2	glycosylation					899:911	glycosylation	899:911	glycosylation	899:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	56	gly	glycosylation	899:911	arg2	site					891:894	one engineered site	876:894	one engineered site of glycosylation	876:911	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	4	57	theme	system	678:683	arg1	effectiveness					627:639	The effectiveness	623:639	The effectiveness of this two-plasmid synthetic operon system	623:683	The effectiveness of this two-plasmid synthetic operon system has been tested on three proteins with therapeutic potential: the native and an engineered version of the naturally O-glycosylated human interferon α-2b, as well as human growth hormone with one engineered site of glycosylation.					
30503285	0	58	theme	Proteins	62:69	arg1	Production					36:45	Production	36:45	Production of Therapeutic Proteins	36:69	A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.					
30503285	5	59	theme	O-glycan	1126:1133	arg1	structures					1135:1144	more complex O-glycan structures	1113:1144	more complex O-glycan structures in E. coli	1113:1155	Having established proof of principle for the addition of the core-1 glycan onto proteins, we are now developing this system as a platform for producing and modifying human protein therapeutics with more complex O-glycan structures in E. coli.					
30503285	3	60	theme	latter	383:388	arg1	plasmid					390:396	The latter plasmid	379:396	The latter plasmid	379:396	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	60	theme	latter	383:388	arg1	-4-epimerase					516:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase	470:527	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	60	theme	latter	383:388	arg1	transferase					447:457	human polypeptide N-acetylgalactosaminyl transferase	406:457	human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin	406:620	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30503285	3	61	theme	Campylobacter	540:552	arg1	jejuni					554:559	Campylobacter jejuni	540:559	Campylobacter jejuni	540:559	The latter plasmid encodes human polypeptide N-acetylgalactosaminyl transferase as well as a β1,3-galactosyl transferase and UDP-Glc(NAc)-4-epimerase, both from Campylobacter jejuni, and a disulfide bond isomerase of bacterial or human origin.					
30146545	0	0	theme	Targeted	89:96	arg1	Analysis					104:111	Targeted LC-MS Analysis	89:111	Targeted LC-MS Analysis	89:111	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	2	1	gly	glycoproteins	279:291	arg1	glycoproteins					279:291	glycoproteins	279:291	glycoproteins	279:291	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	2	2	theme	glycosidic	343:352	arg1	linkages					354:361	the glycosidic linkages	339:361	the glycosidic linkages of a glycan	339:373	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	3	3	from	glycans	511:517	arg1	alditols					490:497	partially O-methylated alditols	467:497	partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis	467:546	In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported.					
30146545	3	3	from	glycans	511:517	arg1	PMAs					500:503	PMAs	500:503	PMAs	500:503	In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported.					
30146545	6	4	theme	Fetuin	765:770	arg1	N-glycan					772:779	Fetuin N-glycan	765:779	Fetuin N-glycan	765:779	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	6	5	theme	antigen	792:798	arg1	carbohydrates					800:812	Lewisa antigen carbohydrates	785:812	Lewisa antigen carbohydrates	785:812	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	0	6	theme	LC-MS	98:102	arg1	Analysis					104:111	Targeted LC-MS Analysis	89:111	Targeted LC-MS Analysis	89:111	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	6	7	attach	derived	752:758	arg2	PMAs					747:750	PMAs	747:750	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates	747:812	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	6	7	attach	derived	752:758	arg1	N-glycan					772:779	Fetuin N-glycan	765:779	Fetuin N-glycan	765:779	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	6	7	attach	derived	752:758	arg1	carbohydrates					800:812	Lewisa antigen carbohydrates	785:812	Lewisa antigen carbohydrates	785:812	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	6	8	theme	Lewisa	785:790	arg1	carbohydrates					800:812	Lewisa antigen carbohydrates	785:812	Lewisa antigen carbohydrates	785:812	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	2	9	theme	structures	265:274	arg1	nature					248:253	the complex nature	236:253	the complex nature of glycan structures on glycoproteins	236:291	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	7	10	with	conjunction	997:1007	arg1	platform					1037:1044	a LC-MS-based analysis platform	1014:1044	a LC-MS-based analysis platform	1014:1044	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	5	11	theme	complete	712:719	arg1	hydrolysis					721:730	complete hydrolysis	712:730	complete hydrolysis	712:730	PMAs were then produced via complete hydrolysis and reduction.					
30146545	0	12	from	Residues	56:63	arg1	Glycans					78:84	Mammalian Glycans	68:84	Mammalian Glycans	68:84	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	2	13	theme	considerable	391:402	arg1	interest					404:411	considerable interest	391:411	considerable interest	391:411	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	2	14	theme	glycan	258:263	arg1	structures					265:274	glycan structures	258:274	glycan structures	258:274	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	2	15	theme	glycan	368:373	arg1	linkages					354:361	the glycosidic linkages	339:361	the glycosidic linkages of a glycan	339:373	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	7	16	theme	derivatization	926:939	arg1	step					941:944	an additional derivatization step	912:944	an additional derivatization step for GC analysis	912:960	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	0	17	theme	Specific	12:19	arg1	Residues					56:63	Specific and Differentially Linked Glycosyl Residues	12:63	Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans	12:84	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	1	18	from	widespread	133:142	arg1	nature					147:152	nature	147:152	nature	147:152	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	1	19	theme	large	168:172	arg1	number					174:179	a large number	166:179	a large number of monosaccharides linked via glycosidic bonds	166:226	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	2	20	dep	configuration	308:320	arg1	the					304:306	the	304:306	the	304:306	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	4	21	theme	methylation	635:645	arg1	levels					625:630	the levels	621:630	the levels of methylation	621:645	N-Glycans were first per-methylated with methyl iodide, and the levels of methylation were further confirmed by MALDI-TOF.					
30146545	1	22	from	nature	147:152	arg1	widespread					133:142	widespread	133:142	widespread	133:142	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	2	23	theme	interest	404:411	arg1	subject					380:386	a subject	378:386	a subject of considerable interest	378:411	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	3	24	theme	LC-MS	533:537	arg1	analysis					539:546	LC-MS analysis	533:546	LC-MS analysis	533:546	In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported.					
30146545	2	25	from	nature	248:253	arg1	glycoproteins					279:291	glycoproteins	279:291	glycoproteins	279:291	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	1	26	theme	monosaccharides	184:198	arg1	number					174:179	a large number	166:179	a large number of monosaccharides linked via glycosidic bonds	166:226	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	0	27	theme	Glycosyl	47:54	arg1	Residues					56:63	Specific and Differentially Linked Glycosyl Residues	12:63	Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans	12:84	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	7	28	theme	GC	950:951	arg1	analysis					953:960	GC analysis	950:960	GC analysis	950:960	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	7	29	from	use	990:992	arg1	conjunction					997:1007	conjunction	997:1007	conjunction with a LC-MS-based analysis platform	997:1044	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	0	30	theme	Linked	40:45	arg1	Residues					56:63	Specific and Differentially Linked Glycosyl Residues	12:63	Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans	12:84	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	3	31	theme	O-methylated	477:488	arg1	alditols					490:497	partially O-methylated alditols	467:497	partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis	467:546	In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported.					
30146545	3	31	theme	O-methylated	477:488	arg1	PMAs					500:503	PMAs	500:503	PMAs	500:503	In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported.					
30146545	7	32	theme	LC-MS-based	1016:1026	arg1	platform					1037:1044	a LC-MS-based analysis platform	1014:1044	a LC-MS-based analysis platform	1014:1044	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	6	33	theme	LC-MS	844:848	arg1	analysis					850:857	LC-MS analysis	844:857	LC-MS analysis	844:857	PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis.					
30146545	7	34	theme	analysis	1028:1035	arg1	platform					1037:1044	a LC-MS-based analysis platform	1014:1044	a LC-MS-based analysis platform	1014:1044	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	7	35	theme	additional	915:924	arg1	step					941:944	an additional derivatization step	912:944	an additional derivatization step for GC analysis	912:960	This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.					
30146545	1	36	theme	glycosidic	211:220	arg1	bonds					222:226	glycosidic bonds	211:226	glycosidic bonds	211:226	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	2	37	theme	complex	240:246	arg1	nature					248:253	the complex nature	236:253	the complex nature of glycan structures on glycoproteins	236:291	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	1	38	attach	linked	200:205	arg3	bonds					222:226	glycosidic bonds	211:226	glycosidic bonds	211:226	Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds.					
30146545	4	39	theme	methyl	602:607	arg1	iodide					609:614	methyl iodide	602:614	methyl iodide	602:614	N-Glycans were first per-methylated with methyl iodide, and the levels of methylation were further confirmed by MALDI-TOF.					
30146545	0	40	theme	Mammalian	68:76	arg1	Glycans					78:84	Mammalian Glycans	68:84	Mammalian Glycans	68:84	Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis.					
30146545	2	41	theme	linkages	354:361	arg1	positions					326:334	positions	326:334	positions	326:334	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
30146545	2	41	theme	linkages	354:361	arg1	configuration					308:320	configuration	308:320	configuration	308:320	Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest.					
29698801	2	0	theme	immunoglobulin	339:352	arg1	G					354:354	immunoglobulin G	339:354	immunoglobulin G (IgG)	339:360	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	2	0	theme	immunoglobulin	339:352	arg1	IgG					357:359	IgG	357:359	IgG	357:359	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	10	1	theme	colorectal	1615:1624	arg1	cancer					1626:1631	colorectal cancer	1615:1631	colorectal cancer	1615:1631	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	2	2	theme	Altered	254:260	arg1	glycosylation					262:274	Altered glycosylation	254:274	Altered glycosylation	254:274	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	1	3	theme	incidence	237:245	arg1	rates					247:251	incidence rates	237:251	incidence rates	237:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	2	4	from	changes	328:334	arg1	G					354:354	immunoglobulin G	339:354	immunoglobulin G (IgG)	339:360	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	2	4	from	changes	328:334	arg1	IgG					357:359	IgG	357:359	IgG	357:359	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	5	5	theme	them	789:792	arg1	five					777:780	five	777:780	five	777:780	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	5	5	theme	them	789:792	arg1	them					789:792	them	789:792	them	789:792	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	10	6	theme	patients	1605:1612	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	11	7	theme	serum	1732:1736	arg1	glycomes					1752:1759	serum comprehensive glycomes	1732:1759	serum comprehensive glycomes	1732:1759	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	7	8	theme	analysis	1100:1107	arg1	performance					1055:1065	Further diagnostic performance	1036:1065	Further diagnostic performance of receiver operator curve (ROC) analysis	1036:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	14	9	theme	large-scale	2488:2498	arg1	study					2500:2504	large-scale study	2488:2504	large-scale study	2488:2504	Besides, it may be useful for large-scale study.					
29698801	3	10	theme	IgG	528:530	arg1	N-glycans					532:540	serum IgG N-glycans	522:540	serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set	522:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	1	11	theme	worldwide	158:166	arg1	one					127:129	one	127:129	one	127:129	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	1	11	theme	worldwide	158:166	arg1	worldwide					158:166	the most common cancers worldwide	134:166	the most common cancers worldwide	134:166	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	1	11	theme	worldwide	158:166	arg1	cancer					197:202	the fifth most prevalent cancer	172:202	the fifth most prevalent cancer in China with an upward trend in incidence rates	172:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	11	12	from	prognosis	1910:1918	arg1	relevance					1860:1868	biological relevance	1849:1868	biological relevance of glycosylation alterations in disease prognosis	1849:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	10	13	theme	individuals	1644:1654	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	5	14	theme	metastasis	861:870	arg1	stages					878:883	all tumor node metastasis (TNM) stages	846:883	all tumor node metastasis (TNM) stages	846:883	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	6	15	theme	obvious	957:963	arg1	differentiation					965:979	obvious differentiation	957:979	obvious differentiation of benign and cancer patients from normal individuals	957:1033	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	11	16	from	relevance	1860:1868	arg1	prognosis					1910:1918	disease prognosis	1902:1918	disease prognosis	1902:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	7	17	theme	curve	1088:1092	arg1	analysis					1100:1107	receiver operator curve (ROC) analysis	1070:1107	receiver operator curve (ROC) analysis	1070:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	13	18	theme	Microsoft	2441:2449	arg1	Excel					2451:2455	Microsoft Excel	2441:2455	Microsoft Excel	2441:2455	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	4	19	theme	Statistical	641:651	arg1	analysis					653:660	Statistical analysis	641:660	Statistical analysis	641:660	Statistical analysis showed nine of IgG N-glycans were differentially expressed in disease groups compared with controls.					
29698801	1	20	from	trend	228:232	arg1	rates					247:251	incidence rates	237:251	incidence rates	237:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	3	21	theme	colorectal	596:605	arg1	cohorts					615:621	normal, benign colorectal and CRC cohorts	581:621	normal, benign colorectal and CRC cohorts in discovery set	581:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	1	22	theme	cancer	197:202	arg1	one					127:129	one	127:129	one	127:129	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	1	22	theme	cancer	197:202	arg1	worldwide					158:166	the most common cancers worldwide	134:166	the most common cancers worldwide	134:166	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	1	22	theme	cancer	197:202	arg1	cancer					197:202	the fifth most prevalent cancer	172:202	the fifth most prevalent cancer in China with an upward trend in incidence rates	172:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	12	23	theme	recent	2104:2109	arg1	method					2122:2127	another recent analytical method	2096:2127	another recent analytical method	2096:2127	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	0	24	from	progression	63:73	arg1	Characterization					0:15	Characterization	0:15	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.	0:90	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	13	25	theme	software	2218:2225	arg1	solution					2227:2234	a software solution	2216:2234	a software solution	2216:2234	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	6	26	theme	component	922:930	arg1	PCA					942:944	PCA	942:944	PCA	942:944	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	6	26	theme	component	922:930	arg1	analysis					932:939	Principal component analysis	912:939	Principal component analysis (PCA)	912:945	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	7	27	theme	Further	1036:1042	arg1	performance					1055:1065	Further diagnostic performance	1036:1065	Further diagnostic performance of receiver operator curve (ROC) analysis	1036:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	6	28	theme	normal	1016:1021	arg1	individuals					1023:1033	normal individuals	1016:1033	normal individuals	1016:1033	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	4	29	theme	N-glycans	681:689	arg1	nine					669:672	nine	669:672	nine	669:672	Statistical analysis showed nine of IgG N-glycans were differentially expressed in disease groups compared with controls.					
29698801	4	29	theme	N-glycans	681:689	arg1	N-glycans					681:689	IgG N-glycans	677:689	IgG N-glycans	677:689	Statistical analysis showed nine of IgG N-glycans were differentially expressed in disease groups compared with controls.					
29698801	11	30	theme	detailed	1823:1830	arg1	understanding					1832:1844	more detailed understanding	1818:1844	more detailed understanding of biological relevance of glycosylation alterations in disease prognosis	1818:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	13	31	theme	files	2201:2205	arg1	processing					2175:2184	a fast and easy data processing	2154:2184	a fast and easy data processing of MS exported files	2154:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	5	32	theme	CRC	830:832	arg1	patients					834:841	CRC patients	830:841	CRC patients	830:841	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	6	33	theme	patients	1002:1009	arg1	differentiation					965:979	obvious differentiation	957:979	obvious differentiation of benign and cancer patients from normal individuals	957:1033	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	11	34	theme	glycosylation	1873:1885	arg1	alterations					1887:1897	glycosylation alterations	1873:1897	glycosylation alterations in disease prognosis	1873:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	1	35	theme	common	143:148	arg1	worldwide					158:166	the most common cancers worldwide	134:166	the most common cancers worldwide	134:166	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	12	36	from	preparation	2030:2040	arg1	stress					2011:2016	manual stress	2004:2016	manual stress from sample preparation	2004:2040	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	0	37	from	Characterization	0:15	arg1	progression					63:73	colorectal cancer progression	45:73	colorectal cancer progression	45:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	6	38	from	individuals	1023:1033	arg1	differentiation					965:979	obvious differentiation	957:979	obvious differentiation of benign and cancer patients from normal individuals	957:1033	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	11	39	theme	relevance	1860:1868	arg1	understanding					1832:1844	more detailed understanding	1818:1844	more detailed understanding of biological relevance of glycosylation alterations in disease prognosis	1818:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	11	40	gly	glycoprotein	1790:1801	arg1	glycoprotein					1790:1801	specific serum glycoprotein	1775:1801	specific serum glycoprotein	1775:1801	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	8	41	theme	validation	1360:1369	arg1	set					1371:1373	validation set	1360:1373	validation set	1360:1373	Significantly, this result has been verified in validation set.					
29698801	13	42	theme	MS	2189:2190	arg1	files					2201:2205	MS exported files	2189:2205	MS exported files	2189:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	13	43	with	operation	2426:2434	arg1	Excel					2451:2455	Microsoft Excel	2441:2455	Microsoft Excel	2441:2455	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	0	44	from	profile	34:40	arg1	progression					63:73	colorectal cancer progression	45:73	colorectal cancer progression	45:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	3	45	attach	derived	542:548	arg1	individuals					555:565	individuals	555:565	individuals consisting of normal, benign colorectal and CRC cohorts in discovery set	555:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	3	45	attach	derived	542:548	arg2	N-glycans					532:540	serum IgG N-glycans	522:540	serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set	522:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	12	46	theme	manual	2004:2009	arg1	stress					2011:2016	manual stress	2004:2016	manual stress from sample preparation	2004:2040	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	0	47	theme	IgG	20:22	arg1	profile					34:40	IgG N-glycome profile	20:40	IgG N-glycome profile in colorectal cancer progression	20:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	1	48	with	cancer	197:202	arg1	trend					228:232	an upward trend	218:232	an upward trend in incidence rates	218:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	11	49	theme	serum	1784:1788	arg1	glycoprotein					1790:1801	specific serum glycoprotein	1775:1801	specific serum glycoprotein	1775:1801	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	5	50	theme	node	856:859	arg1	metastasis					861:870	tumor node metastasis	850:870	all tumor node metastasis (TNM) stages	846:883	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	5	50	theme	node	856:859	arg1	TNM					873:875	TNM	873:875	TNM	873:875	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	0	51	theme	profile	34:40	arg1	Characterization					0:15	Characterization	0:15	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.	0:90	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	10	52	theme	N-glycome	1564:1572	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	3	53	theme	glycosylation	466:478	arg1	association					451:461	the association	447:461	the association of glycosylation with CRC prognosis	447:497	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	7	54	theme	area	1151:1154	arg1	score					1178:1182	accurate area under the curve (AUC) score	1142:1182	accurate area under the curve (AUC) score	1142:1182	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	12	55	theme	high-throughput	1939:1953	arg1	MADLI-TOF-MS					1966:1977	MADLI-TOF-MS	1966:1977	MADLI-TOF-MS	1966:1977	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	12	55	theme	high-throughput	1939:1953	arg1	technique					1955:1963	the high-throughput technique	1935:1963	the high-throughput technique	1935:1963	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	3	56	theme	CRC	611:613	arg1	cohorts					615:621	normal, benign colorectal and CRC cohorts	581:621	normal, benign colorectal and CRC cohorts in discovery set	581:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	10	57	theme	In-depth	1551:1558	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	13	58	theme	data	2170:2173	arg1	processing					2175:2184	a fast and easy data processing	2154:2184	a fast and easy data processing of MS exported files	2154:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	2	59	theme	functional	317:326	arg1	changes					328:334	the structural and functional changes	298:334	the structural and functional changes in immunoglobulin G (IgG)	298:360	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	9	60	theme	IgG	1386:1388	arg1	analysis					1406:1413	IgG N-glycosylation analysis	1386:1413	IgG N-glycosylation analysis	1386:1413	Moreover, IgG N-glycosylation analysis suggested that core-fucosylation, sialylation and sialo core-fucosylation were possibly related to the development of CRC.					
29698801	1	61	with	worldwide	158:166	arg1	trend					228:232	an upward trend	218:232	an upward trend in incidence rates	218:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	10	62	theme	benign	1598:1603	arg1	patients					1605:1612	colorectal benign patients	1587:1612	colorectal benign patients	1587:1612	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	9	63	theme	sialo	1465:1469	arg1	core-fucosylation					1471:1487	sialo core-fucosylation	1465:1487	sialo core-fucosylation	1465:1487	Moreover, IgG N-glycosylation analysis suggested that core-fucosylation, sialylation and sialo core-fucosylation were possibly related to the development of CRC.					
29698801	7	64	theme	accurate	1142:1149	arg1	score					1178:1182	accurate area under the curve (AUC) score	1142:1182	accurate area under the curve (AUC) score	1142:1182	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	6	65	theme	Principal	912:920	arg1	PCA					942:944	PCA	942:944	PCA	942:944	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	6	65	theme	Principal	912:920	arg1	analysis					932:939	Principal component analysis	912:939	Principal component analysis (PCA)	912:945	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	3	66	theme	CRC	485:487	arg1	prognosis					489:497	CRC prognosis	485:497	CRC prognosis	485:497	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	1	67	theme	upward	221:226	arg1	trend					228:232	an upward trend	218:232	an upward trend in incidence rates	218:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	10	68	theme	cancer	1626:1631	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	3	69	with	association	451:461	arg1	prognosis					489:497	CRC prognosis	485:497	CRC prognosis	485:497	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	3	70	theme	serum	522:526	arg1	N-glycans					532:540	serum IgG N-glycans	522:540	serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set	522:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	1	71	theme	cancers	150:156	arg1	worldwide					158:166	the most common cancers worldwide	134:166	the most common cancers worldwide	134:166	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	0	72	theme	colorectal	45:54	arg1	progression					63:73	colorectal cancer progression	45:73	colorectal cancer progression	45:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	11	73	theme	comprehensive	1738:1750	arg1	glycomes					1752:1759	serum comprehensive glycomes	1732:1759	serum comprehensive glycomes	1732:1759	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	2	74	theme	structural	302:311	arg1	changes					328:334	the structural and functional changes	298:334	the structural and functional changes in immunoglobulin G (IgG)	298:360	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	12	75	theme	analytical	2111:2120	arg1	method					2122:2127	another recent analytical method	2096:2127	another recent analytical method	2096:2127	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	5	76	dep	them	789:792	arg1	out					782:784	out	782:784	out	782:784	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	10	77	theme	normal	1637:1642	arg1	individuals					1644:1654	normal individuals	1637:1654	normal individuals	1637:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	7	78	theme	operator	1079:1086	arg1	ROC					1095:1097	ROC	1095:1097	ROC	1095:1097	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	7	78	theme	operator	1079:1086	arg1	curve					1088:1092	receiver operator curve	1070:1092	receiver operator curve (ROC) analysis	1070:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	1	79	theme	fifth	176:180	arg1	cancer					197:202	the fifth most prevalent cancer	172:202	the fifth most prevalent cancer in China with an upward trend in incidence rates	172:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	11	80	theme	disease	1902:1908	arg1	prognosis					1910:1918	disease prognosis	1902:1918	disease prognosis	1902:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	1	81	theme	prevalent	187:195	arg1	cancer					197:202	the fifth most prevalent cancer	172:202	the fifth most prevalent cancer in China with an upward trend in incidence rates	172:251	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	13	82	theme	usual	2420:2424	arg1	operation					2426:2434	usual operation	2420:2434	usual operation with Microsoft Excel	2420:2455	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	11	83	from	alterations	1887:1897	arg1	prognosis					1910:1918	disease prognosis	1902:1918	disease prognosis	1902:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	10	84	theme	expression	1679:1688	arg1	levels					1690:1695	expression levels	1679:1695	expression levels of N-glycosylation	1679:1714	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	7	85	theme	diagnostic	1044:1053	arg1	performance					1055:1065	Further diagnostic performance	1036:1065	Further diagnostic performance of receiver operator curve (ROC) analysis	1036:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	3	86	theme	normal	581:586	arg1	cohorts					615:621	normal, benign colorectal and CRC cohorts	581:621	normal, benign colorectal and CRC cohorts in discovery set	581:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	1	87	from	cancer	197:202	arg1	China					207:211	China	207:211	China	207:211	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	13	88	theme	glycan	2408:2413	arg1	percentage					2389:2398	the average percentage	2377:2398	the average percentage of each glycan	2377:2413	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	9	89	theme	CRC	1533:1535	arg1	development					1518:1528	the development	1514:1528	the development of CRC	1514:1535	Moreover, IgG N-glycosylation analysis suggested that core-fucosylation, sialylation and sialo core-fucosylation were possibly related to the development of CRC.					
29698801	4	90	theme	disease	724:730	arg1	groups					732:737	disease groups	724:737	disease groups	724:737	Statistical analysis showed nine of IgG N-glycans were differentially expressed in disease groups compared with controls.					
29698801	11	91	theme	biological	1849:1858	arg1	relevance					1860:1868	biological relevance	1849:1868	biological relevance of glycosylation alterations in disease prognosis	1849:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	13	92	theme	average	2381:2387	arg1	percentage					2389:2398	the average percentage	2377:2398	the average percentage of each glycan	2377:2413	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	4	93	theme	IgG	677:679	arg1	N-glycans					681:689	IgG N-glycans	677:689	IgG N-glycans	677:689	Statistical analysis showed nine of IgG N-glycans were differentially expressed in disease groups compared with controls.					
29698801	12	94	theme	information	2057:2067	arg1	acquisition					2069:2079	information acquisition	2057:2079	information acquisition	2057:2079	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	1	95	from	worldwide	158:166	arg1	China					207:211	China	207:211	China	207:211	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	6	96	theme	cancer	995:1000	arg1	patients					1002:1009	benign and cancer patients	984:1009	patients	1002:1009	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	11	97	theme	alterations	1887:1897	arg1	relevance					1860:1868	biological relevance	1849:1868	biological relevance of glycosylation alterations in disease prognosis	1849:1918	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	7	98	theme	preferable	1189:1198	arg1	sensitivity					1200:1210	preferable sensitivity	1189:1210	preferable sensitivity	1189:1210	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	6	99	theme	benign	984:989	arg1	patients					1002:1009	benign and cancer patients	984:1009	patients	1002:1009	Principal component analysis (PCA) indicated obvious differentiation of benign and cancer patients from normal individuals.					
29698801	12	100	theme	sample	2023:2028	arg1	preparation					2030:2040	sample preparation	2023:2040	sample preparation	2023:2040	Additionally, the high-throughput technique, MADLI-TOF-MS could absolutely relieve manual stress from sample preparation and accelerate information acquisition as compared to another recent analytical method.					
29698801	3	101	from	cohorts	615:621	arg1	set					636:638	discovery set	626:638	discovery set	626:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	13	102	theme	exported	2192:2199	arg1	files					2201:2205	MS exported files	2189:2205	MS exported files	2189:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	1	103	theme	Colorectal	92:101	arg1	cancer					103:108	Colorectal cancer	92:108	Colorectal cancer (CRC)	92:114	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	1	103	theme	Colorectal	92:101	arg1	CRC					111:113	CRC	111:113	CRC	111:113	Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates.					
29698801	13	104	contain	had	2243:2245	arg2	advantage					2251:2259	the advantage	2247:2259	the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel	2247:2455	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	13	104	contain	had	2243:2245	arg1	solution					2227:2234	a software solution	2216:2234	a software solution	2216:2234	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	10	105	theme	N-glycosylation	1700:1714	arg1	levels					1690:1695	expression levels	1679:1695	expression levels of N-glycosylation	1679:1714	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	0	106	theme	N-glycome	24:32	arg1	profile					34:40	IgG N-glycome profile	20:40	IgG N-glycome profile in colorectal cancer progression	20:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	3	107	dep	normal	581:586	arg1	benign					589:594	benign	589:594	benign	589:594	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	11	108	theme	specific	1775:1782	arg1	glycoprotein					1790:1801	specific serum glycoprotein	1775:1801	specific serum glycoprotein	1775:1801	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	2	109	theme	disease	399:405	arg1	progression					407:417	disease progression	399:417	disease progression	399:417	Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression.					
29698801	7	110	theme	CRC	1295:1297	arg1	progression					1299:1309	CRC progression	1295:1309	CRC progression	1295:1309	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	3	111	theme	discovery	626:634	arg1	set					636:638	discovery set	626:638	discovery set	626:638	In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set.					
29698801	13	112	theme	fast	2156:2159	arg1	processing					2175:2184	a fast and easy data processing	2154:2184	a fast and easy data processing of MS exported files	2154:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	0	113	theme	cancer	56:61	arg1	progression					63:73	colorectal cancer progression	45:73	colorectal cancer progression	45:73	Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS.					
29698801	13	114	theme	interesting	2330:2340	arg1	peaks					2342:2346	the interesting peaks	2326:2346	the interesting peaks	2326:2346	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	7	115	theme	receiver	1070:1077	arg1	ROC					1095:1097	ROC	1095:1097	ROC	1095:1097	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	7	115	theme	receiver	1070:1077	arg1	curve					1088:1092	receiver operator curve	1070:1092	receiver operator curve (ROC) analysis	1070:1107	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	10	116	theme	IgG	1560:1562	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	13	117	theme	easy	2165:2168	arg1	processing					2175:2184	a fast and easy data processing	2154:2184	a fast and easy data processing of MS exported files	2154:2205	Moreover, we introduced a fast and easy data processing of MS exported files based on a software solution, which had the advantage of avoiding time consuming in manually searching and calculating the interesting peaks, and automatically producing the average percentage of each glycan over usual operation with Microsoft Excel.					
29698801	10	118	theme	SIGNIFICANCE	1538:1549	arg1	profiling					1574:1582	SIGNIFICANCE In-depth IgG N-glycome profiling	1538:1582	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals	1538:1654	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
29698801	11	119	theme	glycoprotein	1790:1801	arg1	profiling					1762:1770	profiling	1762:1770	profiling of specific serum glycoprotein	1762:1801	Differing from serum comprehensive glycomes, profiling of specific serum glycoprotein contributes to more detailed understanding of biological relevance of glycosylation alterations in disease prognosis.					
29698801	9	120	theme	N-glycosylation	1390:1404	arg1	analysis					1406:1413	IgG N-glycosylation analysis	1386:1413	IgG N-glycosylation analysis	1386:1413	Moreover, IgG N-glycosylation analysis suggested that core-fucosylation, sialylation and sialo core-fucosylation were possibly related to the development of CRC.					
29698801	5	121	theme	tumor	850:854	arg1	metastasis					861:870	tumor node metastasis	850:870	all tumor node metastasis (TNM) stages	846:883	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	5	121	theme	tumor	850:854	arg1	TNM					873:875	TNM	873:875	TNM	873:875	Additionally, five out of them were still significantly changed in CRC patients at all tumor node metastasis (TNM) stages as compared with controls.					
29698801	7	122	theme	IgG	1251:1253	arg1	N-glycans					1255:1263	these five IgG N-glycans	1240:1263	these five IgG N-glycans	1240:1263	Further diagnostic performance of receiver operator curve (ROC) analysis demonstrated at least moderately accurate area under the curve (AUC) score with preferable sensitivity and specificity, suggesting these five IgG N-glycans were probably correlated with CRC progression.					
29698801	10	123	theme	colorectal	1587:1596	arg1	patients					1605:1612	colorectal benign patients	1587:1612	colorectal benign patients	1587:1612	SIGNIFICANCE In-depth IgG N-glycome profiling of colorectal benign patients, colorectal cancer and normal individuals reveals differentially expression levels of N-glycosylation.					
31000718	6	0	theme	non-physiological	947:963	arg1	glycans					965:971	non-physiological glycans	947:971	non-physiological glycans	947:971	Indeed, non-physiological glycans are produced only when the luminal MTases are challenged with cytosolic substrates.					
31000718	1	1	link	-linked	117:123	arg1	Man9GlcNAc2					125:135	The asparagine (N)-linked Man9GlcNAc2	99:135	The asparagine (N)-linked Man9GlcNAc2	99:135	The asparagine (N)-linked Man9GlcNAc2 is required for glycoprotein folding and secretion.					
31000718	1	2	theme	glycoprotein	153:164	arg1	folding					166:172	glycoprotein folding	153:172	glycoprotein folding	153:172	The asparagine (N)-linked Man9GlcNAc2 is required for glycoprotein folding and secretion.					
31000718	0	3	theme	N-glycans	88:96	arg1	assembly					63:70	assembly	63:70	assembly of high-mannose N-glycans	63:96	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	0	4	link	lipid-linked	22:33	arg1	pathway					51:57	the lipid-linked oligosaccharide pathway	18:57	the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans	18:96	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	6	5	theme	cytosolic	1035:1043	arg1	substrates					1045:1054	cytosolic substrates	1035:1054	cytosolic substrates	1035:1054	Indeed, non-physiological glycans are produced only when the luminal MTases are challenged with cytosolic substrates.					
31000718	7	6	theme	functional	1157:1166	arg1	studies					1168:1174	functional studies	1157:1174	functional studies of the N-linked glycoprotein biosynthesis pathway	1157:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	2	7	dep	Understanding	189:201	arg1	contributes					221:231	contributes	221:231	contributes to these functions	221:250	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	7	8	theme	biosynthesis	1205:1216	arg1	pathway					1218:1224	the N-linked glycoprotein biosynthesis pathway	1179:1224	the N-linked glycoprotein biosynthesis pathway	1179:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	3	9	theme	lipid-linked	510:521	arg1	LLO					540:542	LLO	540:542	LLO	540:542	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	3	9	theme	lipid-linked	510:521	arg1	oligosaccharide					523:537	eukaryotic lipid-linked oligosaccharide	499:537	the eukaryotic lipid-linked oligosaccharide (LLO) pathway	495:551	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	4	10	gly	mannosylation	644:656	arg1	dolichol					665:672	the dolichol mimic	661:678	the dolichol mimic	661:678	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	10	gly	mannosylation	644:656	arg1	GlcNAc2					704:710	phytanyl pyrophosphate GlcNAc2	681:710	phytanyl pyrophosphate GlcNAc2	681:710	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	11	theme	phytanyl	681:688	arg1	dolichol					665:672	the dolichol mimic	661:678	the dolichol mimic	661:678	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	11	theme	phytanyl	681:688	arg1	GlcNAc2					704:710	phytanyl pyrophosphate GlcNAc2	681:710	phytanyl pyrophosphate GlcNAc2	681:710	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	7	12	theme	glycoprotein	1192:1203	arg1	biosynthesis					1205:1216	N-linked glycoprotein biosynthesis	1183:1216	the N-linked glycoprotein biosynthesis pathway	1179:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	0	13	theme	pathway	51:57	arg1	Reconstitution					0:13	Reconstitution	0:13	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.	0:97	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	7	14	theme	LLO	1079:1081	arg1	pathway					1083:1089	the LLO pathway	1075:1089	the LLO pathway	1075:1089	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	7	15	theme	pathway	1083:1089	arg1	Reconstitution					1057:1070	Reconstitution	1057:1070	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro	1057:1124	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	5	16	theme	end	796:798	arg1	products					800:807	end products	796:807	end products that precisely mimic those	796:834	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	2	17	theme	homogenous	314:323	arg1	structure					325:333	a homogenous structure	312:333	a homogenous structure of sufficient quantities for study	312:368	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	2	17	theme	homogenous	314:323	arg1	glycan					302:307	this glycan	297:307	this glycan	297:307	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	7	18	theme	pathway	1218:1224	arg1	studies					1168:1174	functional studies	1157:1174	functional studies of the N-linked glycoprotein biosynthesis pathway	1157:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	7	19	theme	important	1138:1146	arg1	tool					1148:1151	an important tool	1135:1151	an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway	1135:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	3	20	theme	Man9GlcNAc2	430:440	arg1	synthesis					417:425	the high yield chemoenzymatic synthesis	387:425	the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates	387:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	0	21	theme	lipid-linked	22:33	arg1	pathway					51:57	the lipid-linked oligosaccharide pathway	18:57	the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans	18:96	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	5	22	theme	ordered	864:870	arg1	assembly					872:879	ordered assembly	864:879	ordered assembly of LLO	864:886	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	6	23	theme	luminal	1000:1006	arg1	MTases					1008:1013	the luminal MTases	996:1013	the luminal MTases	996:1013	Indeed, non-physiological glycans are produced only when the luminal MTases are challenged with cytosolic substrates.					
31000718	5	24	theme	unique	748:753	arg1	substrates					755:764	unique substrates	748:764	unique substrates	748:764	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	3	25	theme	oligosaccharide	523:537	arg1	pathway					545:551	the eukaryotic lipid-linked oligosaccharide (LLO) pathway	495:551	the eukaryotic lipid-linked oligosaccharide (LLO) pathway	495:551	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	3	26	theme	chemoenzymatic	402:415	arg1	synthesis					417:425	the high yield chemoenzymatic synthesis	387:425	the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates	387:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	2	27	theme	sufficient	338:347	arg1	quantities					349:358	sufficient quantities	338:358	sufficient quantities for study	338:368	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	5	28	theme	LLO	884:886	arg1	assembly					872:879	ordered assembly	864:879	ordered assembly of LLO	864:886	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	7	29	link	N-linked	1183:1190	arg1	biosynthesis					1205:1216	N-linked glycoprotein biosynthesis	1183:1216	the N-linked glycoprotein biosynthesis pathway	1179:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	4	30	theme	pyrophosphate	690:702	arg1	dolichol					665:672	the dolichol mimic	661:678	the dolichol mimic	661:678	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	30	theme	pyrophosphate	690:702	arg1	GlcNAc2					704:710	phytanyl pyrophosphate GlcNAc2	681:710	phytanyl pyrophosphate GlcNAc2	681:710	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	3	31	theme	biosynthetic	450:461	arg1	intermediates					463:475	its biosynthetic intermediates	446:475	its biosynthetic intermediates	446:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	0	32	theme	oligosaccharide	35:49	arg1	pathway					51:57	the lipid-linked oligosaccharide pathway	18:57	the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans	18:96	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	4	33	theme	Endoplasmic	554:564	arg1	reticulum					566:574	Endoplasmic reticulum	554:574	Endoplasmic reticulum mannosyltransferases (MTases)	554:604	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	34	used	used	635:638	arg2	MTases					598:603	MTases	598:603	MTases	598:603	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	34	used	used	635:638	arg2	mannosyltransferases					576:595	Endoplasmic reticulum mannosyltransferases	554:595	Endoplasmic reticulum mannosyltransferases (MTases)	554:604	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	35	dep	dolichol	665:672	arg1	mimic					674:678	mimic	674:678	mimic	674:678	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	3	36	theme	intermediates	463:475	arg1	synthesis					417:425	the high yield chemoenzymatic synthesis	387:425	the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates	387:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	3	37	theme	high	391:394	arg1	synthesis					417:425	the high yield chemoenzymatic synthesis	387:425	the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates	387:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	7	38	gly	glycoprotein	1192:1203	arg1	glycoprotein					1192:1203	N-linked glycoprotein biosynthesis	1183:1216	the N-linked glycoprotein biosynthesis pathway	1179:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	5	39	theme	recombinant	719:729	arg1	MTases					731:736	These recombinant MTases	713:736	These recombinant MTases	713:736	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	2	40	theme	quantities	349:358	arg1	structure					325:333	a homogenous structure	312:333	a homogenous structure of sufficient quantities for study	312:368	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	2	40	theme	quantities	349:358	arg1	glycan					302:307	this glycan	297:307	this glycan	297:307	Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient quantities for study.					
31000718	3	41	theme	yield	396:400	arg1	synthesis					417:425	the high yield chemoenzymatic synthesis	387:425	the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates	387:475	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	1	42	theme	-linked	117:123	arg1	Man9GlcNAc2					125:135	The asparagine (N)-linked Man9GlcNAc2	99:135	The asparagine (N)-linked Man9GlcNAc2	99:135	The asparagine (N)-linked Man9GlcNAc2 is required for glycoprotein folding and secretion.					
31000718	4	43	theme	reticulum	566:574	arg1	MTases					598:603	MTases	598:603	MTases	598:603	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	4	43	theme	reticulum	566:574	arg1	mannosyltransferases					576:595	Endoplasmic reticulum mannosyltransferases	554:595	Endoplasmic reticulum mannosyltransferases (MTases)	554:604	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	3	44	link	lipid-linked	510:521	arg1	LLO					540:542	LLO	540:542	LLO	540:542	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	3	44	link	lipid-linked	510:521	arg1	oligosaccharide					523:537	eukaryotic lipid-linked oligosaccharide	499:537	the eukaryotic lipid-linked oligosaccharide (LLO) pathway	495:551	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	5	45	theme	strict	902:907	arg1	specificity					926:936	the strict enzyme substrate specificity	898:936	the strict enzyme substrate specificity	898:936	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	1	46	gly	glycoprotein	153:164	arg1	glycoprotein					153:164	glycoprotein folding	153:172	glycoprotein folding	153:172	The asparagine (N)-linked Man9GlcNAc2 is required for glycoprotein folding and secretion.					
31000718	3	47	theme	eukaryotic	499:508	arg1	LLO					540:542	LLO	540:542	LLO	540:542	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	3	47	theme	eukaryotic	499:508	arg1	oligosaccharide					523:537	eukaryotic lipid-linked oligosaccharide	499:537	the eukaryotic lipid-linked oligosaccharide (LLO) pathway	495:551	Here, we report the high yield chemoenzymatic synthesis of Man9GlcNAc2 and its biosynthetic intermediates by reconstituting the eukaryotic lipid-linked oligosaccharide (LLO) pathway.					
31000718	0	48	theme	high-mannose	75:86	arg1	N-glycans					88:96	high-mannose N-glycans	75:96	high-mannose N-glycans	75:96	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans.					
31000718	4	49	theme	dolichol	665:672	arg1	mannosylation					644:656	mannosylation	644:656	mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2	644:710	Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosphate GlcNAc2.					
31000718	5	50	theme	enzyme	909:914	arg1	specificity					926:936	the strict enzyme substrate specificity	898:936	the strict enzyme substrate specificity	898:936	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
31000718	7	51	theme	N-linked	1183:1190	arg1	biosynthesis					1205:1216	N-linked glycoprotein biosynthesis	1183:1216	the N-linked glycoprotein biosynthesis pathway	1179:1224	Reconstitution of the LLO pathway to synthesize Man9GlcNAc2 in vitro provides an important tool for functional studies of the N-linked glycoprotein biosynthesis pathway.					
31000718	5	52	theme	substrate	916:924	arg1	specificity					926:936	the strict enzyme substrate specificity	898:936	the strict enzyme substrate specificity	898:936	These recombinant MTases recognize unique substrates and when combined, synthesize end products that precisely mimic those in vivo, demonstrating that ordered assembly of LLO is due to the strict enzyme substrate specificity.					
29941589	0	0	theme	immunological	64:76	arg1	resistance					78:87	immunological resistance	64:87	immunological resistance	64:87	Structure of the Lassa virus glycan shield provides a model for immunological resistance.					
29941589	5	1	theme	attachment	860:869	arg1	subunits					899:906	both the GP1 attachment and GP2 fusion glycoprotein subunits	847:906	subunits	899:906	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	4	2	theme	virus	543:547	arg1	GPC					549:551	Lassa virus GPC	537:551	Lassa virus GPC	537:551	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	4	3	theme	glycosylation	641:653	arg1	sites					655:659	the N-linked glycosylation sites	628:659	the N-linked glycosylation sites presented on the trimeric GPC spike	628:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	4	4	theme	Lassa	537:541	arg1	GPC					549:551	Lassa virus GPC	537:551	Lassa virus GPC	537:551	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	3	5	theme	immune	334:339	arg1	response					341:348	The often inadequate antibody immune response	304:348	The often inadequate antibody immune response elicited by both vaccine and natural infection	304:395	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	7	6	theme	resistant	1366:1374	arg1	targets					1376:1382	immunologically resistant targets	1350:1382	immunologically resistant targets	1350:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	5	7	theme	GP1	856:858	arg1	attachment					860:869	GP1 attachment	856:869	GP1 attachment	856:869	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	5	8	theme	GPC	927:929	arg1	subunits					899:906	both the GP1 attachment and GP2 fusion glycoprotein subunits	847:906	subunits	899:906	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	7	9	gly	glycoprotein	1282:1293	arg1	glycoprotein					1282:1293	the glycoprotein spikes	1278:1300	the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets	1278:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	6	10	theme	humoral	1161:1167	arg1	response					1176:1183	the humoral immune response	1157:1183	the humoral immune response	1157:1183	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	5	11	theme	subunits	899:906	arg1	surface					836:842	the proteinous surface	821:842	the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC	821:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	3	12	theme	inadequate	314:323	arg1	response					341:348	The often inadequate antibody immune response	304:348	The often inadequate antibody immune response elicited by both vaccine and natural infection	304:395	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	5	13	theme	GP2	875:877	arg1	glycoprotein					886:897	GP2 fusion glycoprotein	875:897	GP2 fusion glycoprotein	875:897	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	6	14	theme	limited	1064:1070	arg1	diversification					1083:1097	limited amino acid diversification	1064:1097	limited amino acid diversification around these sites	1064:1116	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	15	theme	accessibility	1016:1028	arg1	role					1133:1136	their role	1127:1136	their role protecting against the humoral immune response	1127:1183	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	15	theme	accessibility	1016:1028	arg1	clusters					951:958	These oligomannose clusters	932:958	These oligomannose clusters	932:958	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	15	theme	accessibility	1016:1028	arg1	result					987:992	a result	985:992	a result of sterically reduced accessibility to glycan processing enzymes	985:1057	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	5	16	theme	glycoprotein	886:897	arg1	subunits					899:906	both the GP1 attachment and GP2 fusion glycoprotein subunits	847:906	subunits	899:906	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	5	17	theme	proteinous	825:834	arg1	surface					836:842	the proteinous surface	821:842	the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC	821:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	3	18	theme	antibody	325:332	arg1	response					341:348	The often inadequate antibody immune response	304:348	The often inadequate antibody immune response elicited by both vaccine and natural infection	304:395	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	7	19	theme	Lassa	1305:1309	arg1	virus					1311:1315	Lassa virus	1305:1315	Lassa virus	1305:1315	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	3	20	from	abundance	434:442	arg1	GPC					477:479	the GPC	473:479	the GPC	473:479	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	4	21	attach	presents	528:535	arg2	system					516:521	a virus-like-particle system	494:521	a virus-like-particle system that presents Lassa virus GPC in a native-like context	494:576	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	4	21	attach	presents	528:535	arg1	context					570:576	a native-like context	556:576	a native-like context	556:576	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	7	22	theme	Old	1327:1329	arg1	arenaviruses					1337:1348	other Old World arenaviruses	1321:1348	other Old World arenaviruses	1321:1348	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	2	23	gly	glycoprotein	238:249	arg1	glycoprotein					238:249	the envelope glycoprotein complex	225:257	the envelope glycoprotein complex (GPC) that extends from the virion envelope	225:301	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	7	24	theme	glycoprotein	1282:1293	arg1	spikes					1295:1300	the glycoprotein spikes	1278:1300	the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets	1278:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	2	25	theme	virion	287:292	arg1	envelope					294:301	the virion envelope	283:301	the virion envelope	283:301	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	6	26	theme	processing	1040:1049	arg1	enzymes					1051:1057	glycan processing enzymes	1033:1057	glycan processing enzymes	1033:1057	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	27	theme	oligomannose	938:949	arg1	role					1133:1136	their role	1127:1136	their role protecting against the humoral immune response	1127:1183	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	27	theme	oligomannose	938:949	arg1	clusters					951:958	These oligomannose clusters	932:958	These oligomannose clusters	932:958	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	6	27	theme	oligomannose	938:949	arg1	result					987:992	a result	985:992	a result of sterically reduced accessibility to glycan processing enzymes	985:1057	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	4	28	theme	GPC	687:689	arg1	spike					691:695	the trimeric GPC spike	674:695	the trimeric GPC spike	674:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	1	29	theme	severe	164:169	arg1	fever					183:187	severe hemorrhagic fever	164:187	severe hemorrhagic fever	164:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	0	30	theme	Lassa	17:21	arg1	glycan					29:34	the Lassa virus glycan shield	13:41	the Lassa virus glycan shield	13:41	Structure of the Lassa virus glycan shield provides a model for immunological resistance.					
29941589	4	31	theme	virus-like-particle	496:514	arg1	system					516:521	a virus-like-particle system	494:521	a virus-like-particle system that presents Lassa virus GPC in a native-like context	494:576	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	1	32	theme	Lassa	90:94	arg1	arenavirus					118:127	an Old World arenavirus	105:127	an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever	105:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	1	32	theme	Lassa	90:94	arg1	virus					96:100	Lassa virus	90:100	Lassa virus	90:100	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	1	33	theme	hemorrhagic	171:181	arg1	fever					183:187	severe hemorrhagic fever	164:187	severe hemorrhagic fever	164:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	5	34	theme	punctuated	788:797	arg1	clusters					799:806	punctuated clusters	788:806	punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC	788:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	6	35	theme	immune	1169:1174	arg1	response					1176:1183	the humoral immune response	1157:1183	the humoral immune response	1157:1183	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	5	36	theme	Lassa	915:919	arg1	GPC					927:929	the Lassa virus GPC	911:929	the Lassa virus GPC	911:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	5	37	theme	virus	921:925	arg1	GPC					927:929	the Lassa virus GPC	911:929	the Lassa virus GPC	911:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	6	38	theme	acid	1078:1081	arg1	diversification					1083:1097	limited amino acid diversification	1064:1097	limited amino acid diversification around these sites	1064:1116	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	0	39	theme	glycan	29:34	arg1	Structure					0:8	Structure	0:8	Structure of the Lassa virus glycan shield	0:41	Structure of the Lassa virus glycan shield provides a model for immunological resistance.					
29941589	5	40	theme	fusion	879:884	arg1	glycoprotein					886:897	GP2 fusion glycoprotein	875:897	GP2 fusion glycoprotein	875:897	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	6	41	theme	reduced	1008:1014	arg1	accessibility					1016:1028	sterically reduced accessibility	997:1028	sterically reduced accessibility to glycan processing enzymes	997:1057	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	7	42	dep	virus	1311:1315	arg1	targets					1376:1382	immunologically resistant targets	1350:1382	immunologically resistant targets	1350:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	4	43	theme	each	620:623	arg1	population					606:615	the composite population	592:615	the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike	592:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	4	44	theme	composite	596:604	arg1	population					606:615	the composite population	592:615	the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike	592:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	0	45	theme	virus	23:27	arg1	glycan					29:34	the Lassa virus glycan shield	13:41	the Lassa virus glycan shield	13:41	Structure of the Lassa virus glycan shield provides a model for immunological resistance.					
29941589	5	46	gly	glycoprotein	886:897	arg1	glycoprotein					886:897	GP2 fusion glycoprotein	875:897	GP2 fusion glycoprotein	875:897	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	7	47	theme	other	1321:1325	arg1	arenaviruses					1337:1348	other Old World arenaviruses	1321:1348	other Old World arenaviruses	1321:1348	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	5	48	theme	oligomannose-type	750:766	arg1	glycans					768:774	underprocessed oligomannose-type glycans	735:774	underprocessed oligomannose-type glycans	735:774	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	5	49	theme	glycans	768:774	arg1	presence					723:730	the presence	719:730	the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC	719:929	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	0	50	dep	glycan	29:34	arg1	shield					36:41	shield	36:41	shield	36:41	Structure of the Lassa virus glycan shield provides a model for immunological resistance.					
29941589	4	51	gly	glycosylation	641:653	arg2	sites					655:659	the N-linked glycosylation sites	628:659	the N-linked glycosylation sites presented on the trimeric GPC spike	628:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	3	52	theme	N-linked	447:454	arg1	glycosylation					456:468	N-linked glycosylation	447:468	N-linked glycosylation	447:468	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	3	53	theme	natural	379:385	arg1	infection					387:395	natural infection	379:395	natural infection	379:395	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	6	54	theme	glycan	1033:1038	arg1	enzymes					1051:1057	glycan processing enzymes	1033:1057	glycan processing enzymes	1033:1057	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	2	55	theme	Vaccine	190:196	arg1	development					198:208	Vaccine development	190:208	Vaccine development	190:208	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	3	56	theme	glycosylation	456:468	arg1	abundance					434:442	the abundance	430:442	the abundance of N-linked glycosylation on the GPC	430:479	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	7	57	theme	arenaviruses	1337:1348	arg1	spikes					1295:1300	the glycoprotein spikes	1278:1300	the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets	1278:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	3	58	link	N-linked	447:454	arg1	glycosylation					456:468	N-linked glycosylation	447:468	N-linked glycosylation	447:468	The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC.					
29941589	1	59	theme	Old	108:110	arg1	arenavirus					118:127	an Old World arenavirus	105:127	an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever	105:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	1	59	theme	Old	108:110	arg1	virus					96:100	Lassa virus	90:100	Lassa virus	90:100	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	4	60	link	N-linked	632:639	arg1	sites					655:659	the N-linked glycosylation sites	628:659	the N-linked glycosylation sites presented on the trimeric GPC spike	628:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	4	61	theme	native-like	558:568	arg1	context					570:576	a native-like context	556:576	a native-like context	556:576	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	2	62	theme	glycoprotein	238:249	arg1	GPC					260:262	GPC	260:262	GPC	260:262	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	2	62	theme	glycoprotein	238:249	arg1	complex					251:257	the envelope glycoprotein complex	225:257	the envelope glycoprotein complex (GPC) that extends from the virion envelope	225:301	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	1	63	theme	World	112:116	arg1	arenavirus					118:127	an Old World arenavirus	105:127	an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever	105:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	1	63	theme	World	112:116	arg1	virus					96:100	Lassa virus	90:100	Lassa virus	90:100	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	7	64	theme	virus	1311:1315	arg1	spikes					1295:1300	the glycoprotein spikes	1278:1300	the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets	1278:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	4	65	theme	trimeric	678:685	arg1	spike					691:695	the trimeric GPC spike	674:695	the trimeric GPC spike	674:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	6	66	theme	amino	1072:1076	arg1	diversification					1083:1097	limited amino acid diversification	1064:1097	limited amino acid diversification around these sites	1064:1116	These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response.					
29941589	2	67	theme	envelope	229:236	arg1	GPC					260:262	GPC	260:262	GPC	260:262	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	2	67	theme	envelope	229:236	arg1	complex					251:257	the envelope glycoprotein complex	225:257	the envelope glycoprotein complex (GPC) that extends from the virion envelope	225:301	Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope.					
29941589	4	68	theme	N-linked	632:639	arg1	sites					655:659	the N-linked glycosylation sites	628:659	the N-linked glycosylation sites presented on the trimeric GPC spike	628:695	Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike.					
29941589	5	69	theme	underprocessed	735:748	arg1	glycans					768:774	underprocessed oligomannose-type glycans	735:774	underprocessed oligomannose-type glycans	735:774	Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC.					
29941589	7	70	theme	structure-based	1215:1229	arg1	blueprint					1231:1239	a structure-based blueprint	1213:1239	a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets	1213:1382	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
29941589	1	71	theme	endemic	129:135	arg1	arenavirus					118:127	an Old World arenavirus	105:127	an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever	105:187	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	1	71	theme	endemic	129:135	arg1	virus					96:100	Lassa virus	90:100	Lassa virus	90:100	Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever.					
29941589	7	72	theme	World	1331:1335	arg1	arenaviruses					1337:1348	other Old World arenaviruses	1321:1348	other Old World arenaviruses	1321:1348	Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.					
30528732	8	0	from	T. reesei	1498:1506	arg1	CBHI					1488:1491	CBHI	1488:1491	CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1488:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	8	0	from	T. reesei	1498:1506	arg1	glycosylation					1471:1483	the glycosylation	1467:1483	the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1467:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	1	1	contain	have	154:157	arg1	enzymes					141:147	glycosylated enzymes	128:147	glycosylated enzymes that have wide applications in fields like biofuels	128:199	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	1	1	contain	have	154:157	arg2	applications					164:175	wide applications	159:175	wide applications	159:175	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	1	1	contain	have	154:157	arg1	Cellulases					113:122	Cellulases	113:122	Cellulases	113:122	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	5	2	gly	glycosylation	691:703	arg1	CBHI					718:721	CBHI	718:721	CBHI	718:721	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	4	3	gly	glycosylation	443:455	arg1	CBHI					470:473	CBHI	470:473	CBHI	470:473	Therefore, the glycosylation of T. reesei CBHI has been a focus of research.					
30528732	8	4	theme	potential	1559:1567	arg1	sites					1569:1573	potential sites	1559:1573	potential sites for modification	1559:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	3	5	theme	cellobiohydrolase	353:369	arg1	I					371:371	cellobiohydrolase I	353:371	cellobiohydrolase I (CBHI)	353:378	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	5	theme	cellobiohydrolase	353:369	arg1	cellulase					402:410	the most important cellulase	383:410	the most important cellulase from T. reesei	383:425	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	5	theme	cellobiohydrolase	353:369	arg1	CBHI					374:377	CBHI	374:377	CBHI	374:377	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	0	6	from	diversity	4:12	arg1	reesei					71:76	Trichoderma reesei	59:76	Trichoderma reesei	59:76	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	6	7	theme	mass	769:772	arg1	investigations					788:801	extensive mass spectrometric investigations	759:801	extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions	759:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	8	from	strains	830:836	arg1	CBHI					806:809	CBHI	806:809	CBHI from two T. reesei strains grown under three conditions	806:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	8	9	theme	CBHI	1488:1491	arg1	glycosylation					1471:1483	the glycosylation	1467:1483	the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1467:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	6	10	theme	spectrometric	774:786	arg1	investigations					788:801	extensive mass spectrometric investigations	759:801	extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions	759:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	11	gly	N-glycosylated	922:935	arg1	N64					914:916	N64	914:916	N64	914:916	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	11	gly	N-glycosylated	922:935	arg1	N45					906:908	N45	906:908	N45	906:908	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	0	12	theme	mass	94:97	arg1	spectrometry					99:110	mass spectrometry	94:110	mass spectrometry	94:110	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	6	13	from	evidence	1085:1092	arg1	mannosylation					1101:1113	the mannosylation	1097:1113	the mannosylation of carbohydrate binding domain (other than the linker adjacent region)	1097:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	14	theme	extensive	759:767	arg1	investigations					788:801	extensive mass spectrometric investigations	759:801	extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions	759:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	5	15	gly	glycosylation	597:609	arg2	four					582:585	four	582:585	four	582:585	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	5	15	gly	glycosylation	597:609	arg2	sites					611:615	the four potential glycosylation sites	578:615	the four potential glycosylation sites	578:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	7	16	theme	cellulose	1391:1399	arg1	binding					1380:1386	the binding	1376:1386	the binding of cellulose	1376:1399	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	0	17	theme	cellobiohydrolase	34:50	arg1	glycosylation					17:29	glycosylation	17:29	glycosylation of cellobiohydrolase I from Trichoderma reesei	17:76	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	7	18	dep	sites	1254:1258	arg1	located					1292:1298	located	1292:1298	located	1292:1298	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	7	18	dep	sites	1254:1258	arg1	involve					1365:1371	involve	1365:1371	involve in the binding of cellulose	1365:1399	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	7	18	dep	sites	1254:1258	arg1	such					1261:1264	such	1261:1264	such	1261:1264	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	8	19	from	glycosylation	1471:1483	arg1	T. reesei					1498:1506	T. reesei	1498:1506	T. reesei	1498:1506	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	7	20	theme	tunnel	1341:1346	arg1	openings					1307:1314	the openings	1303:1314	the openings of the substrate-binding tunnel	1303:1346	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	4	21	theme	research	495:502	arg1	focus					486:490	a focus	484:490	a focus of research	484:502	Therefore, the glycosylation of T. reesei CBHI has been a focus of research.					
30528732	4	21	theme	research	495:502	arg1	glycosylation					443:455	the glycosylation	439:455	the glycosylation of T. reesei CBHI	439:473	Therefore, the glycosylation of T. reesei CBHI has been a focus of research.					
30528732	5	22	theme	potential	587:595	arg1	sites					611:615	the four potential glycosylation sites	578:615	the four potential glycosylation sites	578:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	6	23	theme	domain	1139:1144	arg1	mannosylation					1101:1113	the mannosylation	1097:1113	the mannosylation of carbohydrate binding domain (other than the linker adjacent region)	1097:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	24	dep	domain	1139:1144	arg1	other					1147:1151	other	1147:1151	other	1147:1151	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	8	25	theme	comprehensive	1442:1454	arg1	picture					1456:1462	a full and comprehensive picture	1431:1462	a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1431:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	6	26	theme	type	955:958	arg1	glycans					960:966	high mannose type glycans	942:966	high mannose type glycans	942:966	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	5	27	theme	linker	652:657	arg1	region					659:664	the linker region	648:664	the linker region	648:664	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	5	28	theme	glycosylation	597:609	arg1	sites					611:615	the four potential glycosylation sites	578:615	the four potential glycosylation sites	578:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	6	29	theme	binding	1131:1137	arg1	domain					1139:1144	carbohydrate binding domain	1118:1144	carbohydrate binding domain (other than the linker adjacent region)	1118:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	0	30	theme	glycosylation	17:29	arg1	diversity					4:12	The diversity	0:12	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei	0:76	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	6	31	theme	mannose	947:953	arg1	glycans					960:966	high mannose type glycans	942:966	high mannose type glycans	942:966	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	1	32	gly	glycosylated	128:139	arg1	enzymes					141:147	glycosylated enzymes	128:147	glycosylated enzymes that have wide applications in fields like biofuels	128:199	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	1	32	gly	glycosylated	128:139	arg1	Cellulases					113:122	Cellulases	113:122	Cellulases	113:122	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	5	33	theme	sites	611:615	arg1	three					569:573	three	569:573	three	569:573	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	5	33	theme	sites	611:615	arg1	sites					611:615	the four potential glycosylation sites	578:615	the four potential glycosylation sites	578:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	6	34	theme	carbohydrate	1118:1129	arg1	domain					1139:1144	carbohydrate binding domain	1118:1144	carbohydrate binding domain (other than the linker adjacent region)	1118:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	8	35	theme	full	1433:1436	arg1	picture					1456:1462	a full and comprehensive picture	1431:1462	a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1431:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	3	36	theme	important	392:400	arg1	cellulase					402:410	the most important cellulase	383:410	the most important cellulase from T. reesei	383:425	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	36	theme	important	392:400	arg1	I					371:371	cellobiohydrolase I	353:371	cellobiohydrolase I (CBHI)	353:378	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	6	37	theme	high	942:945	arg1	glycans					960:966	high mannose type glycans	942:966	high mannose type glycans	942:966	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	38	gly	O-glycosylated	1016:1029	arg1	CBHI					996:999	CBHI	996:999	CBHI	996:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	38	gly	O-glycosylated	1016:1029	arg1	O-glycosylated					1016:1029	O-glycosylated	1016:1029	O-glycosylated	1016:1029	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	38	gly	O-glycosylated	1016:1029	arg1	domain					986:991	the catalytic domain	972:991	the catalytic domain of CBHI	972:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	39	theme	several	868:874	arg1	discoveries					880:890	several new discoveries	868:890	several new discoveries	868:890	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	7	40	theme	substrate-binding	1323:1339	arg1	tunnel					1341:1346	the substrate-binding tunnel	1319:1346	the substrate-binding tunnel	1319:1346	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	7	41	from	openings	1307:1314	arg1	located					1292:1298	located	1292:1298	located	1292:1298	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	2	42	gly	glycosylation	235:247	arg1	cellulases					252:261	cellulases	252:261	cellulases	252:261	It has been widely accepted that glycosylation of cellulases impact their performance.					
30528732	5	43	theme	CBHI	718:721	arg1	glycosylation					691:703	glycosylation	691:703	glycosylation of T. reesei CBHI	691:721	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	4	44	theme	CBHI	470:473	arg1	focus					486:490	a focus	484:490	a focus of research	484:502	Therefore, the glycosylation of T. reesei CBHI has been a focus of research.					
30528732	4	44	theme	CBHI	470:473	arg1	glycosylation					443:455	the glycosylation	439:455	the glycosylation of T. reesei CBHI	439:473	Therefore, the glycosylation of T. reesei CBHI has been a focus of research.					
30528732	6	45	from	investigations	788:801	arg1	CBHI					806:809	CBHI	806:809	CBHI from two T. reesei strains grown under three conditions	806:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	3	46	theme	Trichoderma	289:299	arg1	reesei					301:306	Trichoderma reesei	289:306	Trichoderma reesei	289:306	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	46	theme	Trichoderma	289:299	arg1	cellulase-producer					330:347	the most important cellulase-producer	311:347	the most important cellulase-producer	311:347	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	0	47	from	reesei	71:76	arg1	glycosylation					17:29	glycosylation	17:29	glycosylation of cellobiohydrolase I from Trichoderma reesei	17:76	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	0	47	from	reesei	71:76	arg1	diversity					4:12	The diversity	0:12	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei	0:76	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	6	48	dep	made	897:900	arg1	2					969:969	2	969:969	2	969:969	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	48	dep	made	897:900	arg1	1					903:903	1	903:903	1	903:903	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	48	dep	made	897:900	arg1	CBHI					996:999	CBHI	996:999	CBHI	996:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	48	dep	made	897:900	arg1	O-glycosylated					1016:1029	O-glycosylated	1016:1029	O-glycosylated	1016:1029	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	48	dep	made	897:900	arg1	domain					986:991	the catalytic domain	972:991	the catalytic domain of CBHI	972:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	48	dep	made	897:900	arg1	N-glycosylated					922:935	N-glycosylated	922:935	N-glycosylated	922:935	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	49	theme	CBHI	996:999	arg1	CBHI					996:999	CBHI	996:999	CBHI	996:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	49	theme	CBHI	996:999	arg1	O-glycosylated					1016:1029	O-glycosylated	1016:1029	O-glycosylated	1016:1029	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	49	theme	CBHI	996:999	arg1	domain					986:991	the catalytic domain	972:991	the catalytic domain of CBHI	972:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	3	50	theme	important	320:328	arg1	reesei					301:306	Trichoderma reesei	289:306	Trichoderma reesei	289:306	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	50	theme	important	320:328	arg1	cellulase-producer					330:347	the most important cellulase-producer	311:347	the most important cellulase-producer	311:347	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	5	51	theme	three	569:573	arg1	N-glycosylation					550:564	N-glycosylation	550:564	N-glycosylation of three of the four potential glycosylation sites	550:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	7	52	theme	glycosylation	1240:1252	arg1	sites					1254:1258	several glycosylation sites	1232:1258	several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose	1232:1399	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	8	53	from	picture	1456:1462	arg1	glycosylation					1471:1483	the glycosylation	1467:1483	the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1467:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	5	54	gly	N-glycosylation	550:564	arg1	three					569:573	three	569:573	three	569:573	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	5	54	gly	N-glycosylation	550:564	arg1	sites					611:615	the four potential glycosylation sites	578:615	the four potential glycosylation sites	578:615	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	7	55	gly	glycosylation	1240:1252	arg2	sites					1254:1258	several glycosylation sites	1232:1258	several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose	1232:1399	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	0	56	gly	glycosylation	17:29	arg1	cellobiohydrolase					34:50	cellobiohydrolase I	34:52	cellobiohydrolase I	34:52	The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.					
30528732	1	57	theme	glycosylated	128:139	arg1	enzymes					141:147	glycosylated enzymes	128:147	glycosylated enzymes that have wide applications in fields like biofuels	128:199	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	1	57	theme	glycosylated	128:139	arg1	Cellulases					113:122	Cellulases	113:122	Cellulases	113:122	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	6	58	theme	linker	1162:1167	arg1	region					1178:1183	the linker adjacent region	1158:1183	the linker adjacent region	1158:1183	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	5	59	theme	full	675:678	arg1	picture					680:686	a full picture	673:686	a full picture of glycosylation of T. reesei CBHI	673:721	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	6	60	theme	new	876:878	arg1	discoveries					880:890	several new discoveries	868:890	several new discoveries	868:890	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	61	with	N-glycosylated	922:935	arg1	glycans					960:966	high mannose type glycans	942:966	high mannose type glycans	942:966	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	8	62	gly	glycosylation	1471:1483	arg1	CBHI					1488:1491	CBHI	1488:1491	CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification	1488:1590	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
30528732	5	63	theme	glycosylation	691:703	arg1	picture					680:686	a full picture	673:686	a full picture of glycosylation of T. reesei CBHI	673:721	However, investigations have been focused on N-glycosylation of three of the four potential glycosylation sites, as well as O-glycosylation on the linker region, while a full picture of glycosylation of T. reesei CBHI is still needed.					
30528732	6	64	with	O-glycosylated	1016:1029	arg1	hexoses					1036:1042	hexoses	1036:1042	hexoses	1036:1042	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	64	with	O-glycosylated	1016:1029	arg1	N-acetylhexosamines					1048:1066	N-acetylhexosamines	1048:1066	N-acetylhexosamines	1048:1066	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	65	dep	evidence	1085:1092	arg1	3					1069:1069	3	1069:1069	3	1069:1069	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	7	66	theme	several	1232:1238	arg1	sites					1254:1258	several glycosylation sites	1232:1258	several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose	1232:1399	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	6	67	theme	T. reesei	820:828	arg1	strains					830:836	two T. reesei strains	816:836	two T. reesei strains grown under three conditions	816:865	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	68	theme	adjacent	1169:1176	arg1	region					1178:1183	the linker adjacent region	1158:1183	the linker adjacent region	1158:1183	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	69	theme	experimental	1072:1083	arg1	evidence					1085:1092	3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region)	1069:1184	3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region)	1069:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	70	theme	catalytic	976:984	arg1	CBHI					996:999	CBHI	996:999	CBHI	996:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	70	theme	catalytic	976:984	arg1	O-glycosylated					1016:1029	O-glycosylated	1016:1029	O-glycosylated	1016:1029	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	70	theme	catalytic	976:984	arg1	domain					986:991	the catalytic domain	972:991	the catalytic domain of CBHI	972:999	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	6	71	gly	mannosylation	1101:1113	arg1	domain					1139:1144	carbohydrate binding domain	1118:1144	carbohydrate binding domain (other than the linker adjacent region)	1118:1184	In this work, with extensive mass spectrometric investigations on CBHI from two T. reesei strains grown under three conditions, several new discoveries were made: 1) N45 and N64 are N-glycosylated with high mannose type glycans; 2) the catalytic domain of CBHI is extensively O-glycosylated with hexoses and N-acetylhexosamines; 3) experimental evidence on the mannosylation of carbohydrate binding domain (other than the linker adjacent region) was found.					
30528732	3	72	from	T. reesei	417:425	arg1	cellulase					402:410	the most important cellulase	383:410	the most important cellulase from T. reesei	383:425	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	72	from	T. reesei	417:425	arg1	I					371:371	cellobiohydrolase I	353:371	cellobiohydrolase I (CBHI)	353:378	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	72	from	T. reesei	417:425	arg1	reesei					301:306	Trichoderma reesei	289:306	Trichoderma reesei	289:306	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	3	72	from	T. reesei	417:425	arg1	cellulase-producer					330:347	the most important cellulase-producer	311:347	the most important cellulase-producer	311:347	Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI) is the most important cellulase from T. reesei.					
30528732	2	73	theme	cellulases	252:261	arg1	glycosylation					235:247	glycosylation	235:247	glycosylation of cellulases	235:261	It has been widely accepted that glycosylation of cellulases impact their performance.					
30528732	1	74	theme	wide	159:162	arg1	applications					164:175	wide applications	159:175	wide applications	159:175	Cellulases are glycosylated enzymes that have wide applications in fields like biofuels.					
30528732	7	75	theme	structural	1202:1211	arg1	analysis					1213:1220	structural analysis	1202:1220	structural analysis	1202:1220	With structural analysis, we found several glycosylation sites (such as T383, S8, and S46) are located at the openings of the substrate-binding tunnel, and potentially involve in the binding of cellulose.					
30528732	8	76	theme	CBHI	1543:1546	arg1	engineering					1528:1538	the engineering	1524:1538	the engineering of CBHI	1524:1546	These investigations provide a full and comprehensive picture on the glycosylation of CBHI from T. reesei, which benefits the engineering of CBHI by raising potential sites for modification.					
31302178	3	0	theme	many	327:330	arg1	groups					341:346	the many reactive groups	323:346	the many reactive groups of the glycan chains	323:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	3	0	theme	many	327:330	arg1	chains					362:367	the glycan chains	351:367	the glycan chains	351:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	8	1	from	mode	1210:1213	arg1	UnityMol					1218:1225	UnityMol	1218:1225	UnityMol	1218:1225	We propose here to transform this analysis method into a visualization mode in UnityMol.					
31302178	4	2	theme	conformational	555:568	arg1	information					570:580	conformational information	555:580	conformational information through exhaustive sampling	555:608	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	3	3	theme	reactive	332:339	arg1	groups					341:346	the many reactive groups	323:346	the many reactive groups of the glycan chains	323:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	3	3	theme	reactive	332:339	arg1	chains					362:367	the glycan chains	351:367	the glycan chains	351:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	5	4	theme	N-glycans	644:652	arg1	impact					634:639	the impact	630:639	the impact of N-glycans	630:652	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	11	5	theme	protein	1522:1528	arg1	surface					1530:1536	the protein surface	1518:1536	the protein surface	1518:1536	By displaying the collected data on the protein surface, one is then able to investigate the protein/glycan interactions.					
31302178	7	6	theme	interactions	1125:1136	arg1	description					1081:1091	an accurate description	1069:1091	an accurate description of the protein topology and its interactions	1069:1136	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	5	7	from	structure	726:734	arg1	visualization					672:684	visualization	672:684	visualization	672:684	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	5	7	from	structure	726:734	arg1	prerequisite					741:752	a prerequisite	739:752	a prerequisite	739:752	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	5	7	from	structure	726:734	arg1	analysis					659:666	analysis	659:666	analysis	659:666	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	3	8	theme	chains	362:367	arg1	groups					341:346	the many reactive groups	323:346	the many reactive groups of the glycan chains	323:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	3	8	theme	chains	362:367	arg1	flexibility					307:317	their high flexibility	296:317	their high flexibility	296:317	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	3	8	theme	chains	362:367	arg1	chains					362:367	the glycan chains	351:367	the glycan chains	351:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	6	9	theme	Umbrella	772:779	arg1	Visualization					781:793	the Umbrella Visualization	768:793	the Umbrella Visualization	768:793	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	6	9	theme	Umbrella	772:779	arg1	method					808:813	a graphical method	796:813	a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory	796:898	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	4	10	from	one	490:492	arg1	biology					497:503	biology	497:503	biology able to overcome this problem	497:533	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	6	11	theme	graphical	798:806	arg1	Visualization					781:793	the Umbrella Visualization	768:793	the Umbrella Visualization	768:793	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	6	11	theme	graphical	798:806	arg1	method					808:813	a graphical method	796:813	a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory	796:898	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	10	12	dep	account	1381:1387	arg1	both					1389:1392	both	1389:1392	both	1389:1392	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	8	13	theme	analysis	1173:1180	arg1	method					1182:1187	this analysis method	1168:1187	this analysis method	1168:1187	We propose here to transform this analysis method into a visualization mode in UnityMol.					
31302178	5	14	theme	protein	718:724	arg1	structure					726:734	protein structure	718:734	protein structure	718:734	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	3	15	dep	in	392:393	arg1	vitro					395:399	vitro	395:399	vitro	395:399	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	7	16	theme	relevant	951:958	arg1	informations					960:971	relevant informations	951:971	relevant informations regarding glycans dynamics and flexibility	951:1014	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	5	17	from	influence	695:703	arg1	structure					726:734	protein structure	718:734	protein structure	718:734	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	5	18	dep	analysis	659:666	arg1	the					655:657	the	655:657	the	655:657	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	6	19	theme	trajectory	889:898	arg1	dynamics					880:887	a molecular dynamics	868:887	a molecular dynamics trajectory	868:898	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	7	20	theme	topology	1108:1115	arg1	description					1081:1091	an accurate description	1069:1091	an accurate description of the protein topology and its interactions	1069:1136	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	3	21	theme	glycan	355:360	arg1	chains					362:367	the glycan chains	351:367	the glycan chains	351:367	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	9	22	theme	C	1302:1302	arg1	#					1303:1303	C#	1302:1303	C#	1302:1303	UnityMol is a molecular editor, viewer and prototyping platform, coded in C#.					
31302178	10	23	theme	statistical	1459:1469	arg1	relevance					1471:1479	their statistical relevance	1453:1479	their statistical relevance	1453:1479	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	0	24	theme	Umbrella	0:7	arg1	Visualization					9:21	Umbrella Visualization	0:21	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.	0:90	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.					
31302178	8	25	theme	visualization	1196:1208	arg1	mode					1210:1213	a visualization mode	1194:1213	a visualization mode in UnityMol	1194:1225	We propose here to transform this analysis method into a visualization mode in UnityMol.					
31302178	6	26	theme	molecular	870:878	arg1	dynamics					880:887	a molecular dynamics	868:887	a molecular dynamics trajectory	868:898	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	10	27	theme	glycan	1442:1447	arg1	antenna					1429:1435	each antenna	1424:1435	each antenna of a glycan and their statistical relevance	1424:1479	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	0	28	theme	glycan	58:63	arg1	flexibility					65:75	glycan flexibility	58:75	glycan flexibility	58:75	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.					
31302178	3	29	theme	challenging	414:424	arg1	task					426:429	a challenging task	412:429	a challenging task	412:429	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	3	30	theme	in	392:393	arg1	methods					401:407	in vitro methods	392:407	in vitro methods	392:407	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	7	31	theme	protein	1100:1106	arg1	topology					1108:1115	the protein topology	1096:1115	the protein topology	1096:1115	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	10	32	theme	main	1398:1401	arg1	positions					1403:1411	the main positions	1394:1411	the main positions adopted by each antenna of a glycan and their statistical relevance	1394:1479	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	3	33	with	glycans	379:385	arg1	methods					401:407	in vitro methods	392:407	in vitro methods	392:407	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	10	34	theme	new	1310:1312	arg1	representation					1314:1327	The new representation	1306:1327	The new representation of glycan chains presented in this study	1306:1368	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	10	35	theme	a	1440:1440	arg1	glycan					1442:1447	a glycan	1440:1447	a glycan	1440:1447	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	6	36	theme	glycan	832:837	arg1	flexibility					849:859	the glycan intrinsic flexibility	828:859	the glycan intrinsic flexibility	828:859	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	5	37	theme	influence	695:703	arg1	visualization					672:684	visualization	672:684	visualization	672:684	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	5	37	theme	influence	695:703	arg1	prerequisite					741:752	a prerequisite	739:752	a prerequisite	739:752	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	5	37	theme	influence	695:703	arg1	analysis					659:666	analysis	659:666	analysis	659:666	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	2	38	theme	glycosylation	202:214	arg1	alterations					187:197	Some alterations	182:197	Some alterations of glycosylation	182:214	Some alterations of glycosylation, such as sialic acid hydrolysis, are related to protein dysfunction.					
31302178	10	39	theme	relevance	1471:1479	arg1	antenna					1429:1435	each antenna	1424:1435	each antenna of a glycan and their statistical relevance	1424:1479	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	9	40	theme	molecular	1242:1250	arg1	editor					1252:1257	a molecular editor	1240:1257	a molecular editor	1240:1257	UnityMol is a molecular editor, viewer and prototyping platform, coded in C#.					
31302178	9	40	theme	molecular	1242:1250	arg1	UnityMol					1228:1235	UnityMol	1228:1235	UnityMol	1228:1235	UnityMol is a molecular editor, viewer and prototyping platform, coded in C#.					
31302178	7	41	dep	glycans	983:989	arg1	flexibility					1004:1014	flexibility	1004:1014	flexibility	1004:1014	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	7	41	dep	glycans	983:989	arg1	dynamics					991:998	dynamics	991:998	dynamics	991:998	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	7	42	theme	accurate	1072:1079	arg1	description					1081:1091	an accurate description	1069:1091	an accurate description of the protein topology and its interactions	1069:1136	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	4	43	theme	useful	456:461	arg1	tool					463:466	a useful tool	454:466	a useful tool	454:466	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	4	43	theme	useful	456:461	arg1	dynamics					442:449	Molecular dynamics	432:449	Molecular dynamics	432:449	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	9	44	theme	prototyping	1271:1281	arg1	platform					1283:1290	prototyping platform	1271:1290	prototyping platform	1271:1290	UnityMol is a molecular editor, viewer and prototyping platform, coded in C#.					
31302178	7	45	theme	further	1027:1033	arg1	development					1035:1045	further development in order to integrate an accurate description of the protein topology and its interactions	1027:1136	further development in order to integrate an accurate description of the protein topology and its interactions	1027:1136	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	4	46	theme	able	505:508	arg1	biology					497:503	biology	497:503	biology able to overcome this problem	497:533	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	6	47	theme	intrinsic	839:847	arg1	flexibility					849:859	the glycan intrinsic flexibility	828:859	the glycan intrinsic flexibility	828:859	We developed the Umbrella Visualization, a graphical method that assigns the glycan intrinsic flexibility during a molecular dynamics trajectory.					
31302178	10	48	theme	glycan	1332:1337	arg1	chains					1339:1344	glycan chains	1332:1344	glycan chains presented in this study	1332:1368	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	0	49	theme	analysis	36:43	arg1	method					26:31	A method	24:31	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.	0:90	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.					
31302178	2	50	theme	sialic	225:230	arg1	hydrolysis					237:246	sialic acid hydrolysis	225:246	sialic acid hydrolysis	225:246	Some alterations of glycosylation, such as sialic acid hydrolysis, are related to protein dysfunction.					
31302178	2	51	theme	acid	232:235	arg1	hydrolysis					237:246	sialic acid hydrolysis	225:246	sialic acid hydrolysis	225:246	Some alterations of glycosylation, such as sialic acid hydrolysis, are related to protein dysfunction.					
31302178	1	52	theme	post-translational	113:130	arg1	modification					132:143	a post-translational modification	111:143	a post-translational modification heavily impacting protein functions	111:179	N-glycosylation is a post-translational modification heavily impacting protein functions.					
31302178	1	52	theme	post-translational	113:130	arg1	N-glycosylation					92:106	N-glycosylation	92:106	N-glycosylation	92:106	N-glycosylation is a post-translational modification heavily impacting protein functions.					
31302178	3	53	theme	high	302:305	arg1	flexibility					307:317	their high flexibility	296:317	their high flexibility	296:317	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	5	54	from	visualization	672:684	arg1	structure					726:734	protein structure	718:734	protein structure	718:734	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	2	55	dep	such	217:220	arg1	as					222:223	as	222:223	as	222:223	Some alterations of glycosylation, such as sialic acid hydrolysis, are related to protein dysfunction.					
31302178	3	56	theme	flexibility	307:317	arg1	task					426:429	a challenging task	412:429	a challenging task	412:429	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	4	57	theme	Molecular	432:440	arg1	tool					463:466	a useful tool	454:466	a useful tool	454:466	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	4	57	theme	Molecular	432:440	arg1	dynamics					442:449	Molecular dynamics	432:449	Molecular dynamics	432:449	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	2	58	theme	protein	264:270	arg1	dysfunction					272:282	protein dysfunction	264:282	protein dysfunction	264:282	Some alterations of glycosylation, such as sialic acid hydrolysis, are related to protein dysfunction.					
31302178	10	59	theme	chains	1339:1344	arg1	representation					1314:1327	The new representation	1306:1327	The new representation of glycan chains presented in this study	1306:1368	The new representation of glycan chains presented in this study takes into account both the main positions adopted by each antenna of a glycan and their statistical relevance.					
31302178	4	60	theme	exhaustive	590:599	arg1	sampling					601:608	exhaustive sampling	590:608	exhaustive sampling	590:608	Molecular dynamics is a useful tool and probably the only one in biology able to overcome this problem and gives access to conformational information through exhaustive sampling.					
31302178	3	61	theme	groups	341:346	arg1	task					426:429	a challenging task	412:429	a challenging task	412:429	Because of their high flexibility and the many reactive groups of the glycan chains, studying glycans with in vitro methods is a challenging task.					
31302178	0	62	dep	Visualization	9:21	arg1	method					26:31	A method	24:31	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.	0:90	Umbrella Visualization: A method of analysis dedicated to glycan flexibility with UnityMol.					
31302178	7	63	theme	density	905:911	arg1	plot					913:916	The density plot	901:916	The density plot generated by this method	901:941	The density plot generated by this method brought relevant informations regarding glycans dynamics and flexibility, but needs further development in order to integrate an accurate description of the protein topology and its interactions.					
31302178	5	64	from	analysis	659:666	arg1	structure					726:734	protein structure	718:734	protein structure	718:734	To better decipher the impact of N-glycans, the analysis and visualization of their influence over time on protein structure is a prerequisite.					
31302178	11	65	from	data	1510:1513	arg1	surface					1530:1536	the protein surface	1518:1536	the protein surface	1518:1536	By displaying the collected data on the protein surface, one is then able to investigate the protein/glycan interactions.					
31302178	11	66	theme	protein/glycan	1575:1588	arg1	interactions					1590:1601	the protein/glycan interactions	1571:1601	the protein/glycan interactions	1571:1601	By displaying the collected data on the protein surface, one is then able to investigate the protein/glycan interactions.					
31302178	1	67	theme	protein	163:169	arg1	functions					171:179	protein functions	163:179	protein functions	163:179	N-glycosylation is a post-translational modification heavily impacting protein functions.					
31302178	11	68	theme	collected	1500:1508	arg1	data					1510:1513	the collected data	1496:1513	the collected data on the protein surface	1496:1536	By displaying the collected data on the protein surface, one is then able to investigate the protein/glycan interactions.					
29330305	3	0	theme	NK	676:677	arg1	cells					679:683	the primary NK cells	664:683	the primary NK cells of three donors	664:699	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	5	1	link	cell-derived	1174:1185	arg1	CD16a					1187:1191	NK cell-derived CD16a	1171:1191	NK cell-derived CD16a	1171:1191	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	9	2	theme	critical	1893:1900	arg1	evidence					1902:1909	critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity	1893:1995	critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity	1893:1995	Furthermore, our study provides critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity.					
29330305	5	3	theme	cell-derived	1174:1185	arg1	CD16a					1187:1191	NK cell-derived CD16a	1171:1191	NK cell-derived CD16a	1171:1191	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	7	4	from	changes	1528:1534	arg1	activity					1547:1554	binding activity	1539:1554	binding activity	1539:1554	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	1	5	with	engagement	288:297	arg1	antibody					304:311	antibody	304:311	antibody clustered on the surface of a pathogen or diseased tissue	304:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	3	6	theme	donors	694:699	arg1	cells					679:683	the primary NK cells	664:683	the primary NK cells of three donors	664:699	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	1	7	theme	abundant	157:164	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	7	theme	abundant	157:164	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	4	8	theme	restricted	813:822	arg1	processing					833:842	restricted N-glycan processing	813:842	restricted N-glycan processing	813:842	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	1	9	theme	Fc	175:176	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	9	theme	Fc	175:176	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	7	10	theme	CD16a	1633:1637	arg1	composition					1646:1656	CD16a glycan composition	1633:1656	CD16a glycan composition	1633:1656	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	2	11	theme	antibody-CD16a	538:551	arg1	interactions					553:564	antibody-CD16a interactions	538:564	antibody-CD16a interactions	538:564	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	5	12	from	CD16a	1249:1253	arg1	different					1218:1226	different	1218:1226	different	1218:1226	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	9	13	theme	CD16a	1940:1944	arg1	composition					1955:1965	CD16a N-glycan composition	1940:1965	CD16a N-glycan composition	1940:1965	Furthermore, our study provides critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity.					
29330305	3	14	theme	large	718:722	arg1	%					781:781	23%	779:781	23%	779:781	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	3	14	theme	large	718:722	arg1	proportion					724:733	a large proportion	716:733	a large proportion of hybrid (22%) and oligomannose N-glycans (23%)	716:782	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	1	15	theme	diseased	355:362	arg1	tissue					364:369	diseased tissue	355:369	diseased tissue	355:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	8	16	theme	primary	1741:1747	arg1	cells					1758:1762	primary human NK cells	1741:1762	primary human NK cells	1741:1762	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	5	17	link	cell-derived	1236:1247	arg1	CD16a					1249:1253	NK cell-derived CD16a	1233:1253	NK cell-derived CD16a	1233:1253	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	8	18	theme	NK	1755:1756	arg1	cells					1758:1762	primary human NK cells	1741:1762	primary human NK cells	1741:1762	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	1	19	theme	human	200:204	arg1	cells					226:230	human natural killer (NK) cells	200:230	human natural killer (NK) cells	200:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	7	20	theme	glycoforms	1605:1614	arg1	spectra					1580:1586	NMR spectra	1576:1586	NMR spectra of the two CD16a glycoforms	1576:1614	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	2	21	theme	asparagine-linked	571:587	arg1	N-glycans					604:612	N-glycans	604:612	N-glycans	604:612	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	21	theme	asparagine-linked	571:587	arg1	carbohydrates					589:601	the asparagine-linked carbohydrates	567:601	the asparagine-linked carbohydrates (N-glycans)	567:613	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	21	theme	asparagine-linked	571:587	arg1	factor					517:522	one significant factor	501:522	one significant factor that promotes antibody-CD16a interactions	501:564	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	5	22	from	CD16a	1279:1283	arg1	different					1218:1226	different	1218:1226	different	1218:1226	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	9	23	theme	antibody-binding	1971:1986	arg1	affinity					1988:1995	antibody-binding affinity	1971:1995	antibody-binding affinity	1971:1995	Furthermore, our study provides critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity.					
29330305	6	24	with	CD16a	1370:1374	arg1	N-glycans					1394:1402	oligomannose N-glycans	1381:1402	oligomannose N-glycans	1381:1402	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	3	25	theme	N-glycans	768:776	arg1	%					781:781	23%	779:781	23%	779:781	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	3	25	theme	N-glycans	768:776	arg1	proportion					724:733	a large proportion	716:733	a large proportion of hybrid (22%) and oligomannose N-glycans (23%)	716:782	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	1	26	theme	γ	129:129	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	26	theme	γ	129:129	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	0	27	from	cells	82:86	arg1	processing					11:20	Restricted processing	0:20	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	0:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	0	27	from	cells	82:86	arg1	N-glycans					50:58	CD16a/Fc γ receptor IIIa N-glycans	25:58	CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	25:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	28	with	coexpression	1062:1073	arg1	γ-chain					1098:1104	the Fc ϵ receptor γ-chain	1080:1104	the Fc ϵ receptor γ-chain in HEK293F cells	1080:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	5	29	theme	Fc	1084:1085	arg1	γ-chain					1098:1104	the Fc ϵ receptor γ-chain	1080:1104	the Fc ϵ receptor γ-chain in HEK293F cells	1080:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	7	30	theme	NMR	1576:1578	arg1	spectra					1580:1586	NMR spectra	1576:1586	NMR spectra of the two CD16a glycoforms	1576:1614	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	2	31	theme	therapeutic	471:481	arg1	outcome					483:489	superior therapeutic outcome	462:489	superior therapeutic outcome	462:489	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	0	32	theme	human	73:77	arg1	cells					82:86	primary human NK cells	65:86	primary human NK cells	65:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	33	theme	receptor	1089:1096	arg1	γ-chain					1098:1104	the Fc ϵ receptor γ-chain	1080:1104	the Fc ϵ receptor γ-chain in HEK293F cells	1080:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	2	34	theme	significant	505:515	arg1	factor					517:522	one significant factor	501:522	one significant factor that promotes antibody-CD16a interactions	501:564	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	34	theme	significant	505:515	arg1	carbohydrates					589:601	the asparagine-linked carbohydrates	567:601	the asparagine-linked carbohydrates (N-glycans)	567:613	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	5	35	theme	cell-derived	1236:1247	arg1	CD16a					1249:1253	NK cell-derived CD16a	1233:1253	NK cell-derived CD16a	1233:1253	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	4	36	theme	complex-type	923:934	arg1	N-glycans					936:944	predominantly complex-type N-glycans	909:944	predominantly complex-type N-glycans	909:944	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	2	37	theme	Therapeutic	372:382	arg1	antibodies					395:404	Therapeutic monoclonal antibodies	372:404	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a	372:455	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	37	theme	Therapeutic	372:382	arg1	mAbs					407:410	mAbs	407:410	mAbs	407:410	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	6	38	theme	CD16a	1316:1320	arg1	composition					1331:1341	CD16a N-glycan composition	1316:1341	CD16a N-glycan composition	1316:1341	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	1	39	theme	pathogen	343:350	arg1	surface					330:336	the surface	326:336	the surface of a pathogen or diseased tissue	326:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	2	40	theme	Fc-mediated	426:436	arg1	affinity					438:445	greater Fc-mediated affinity	418:445	greater Fc-mediated affinity for CD16a	418:455	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	0	41	theme	Restricted	0:9	arg1	processing					11:20	Restricted processing	0:20	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	0:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	8	42	theme	recombinant	1842:1852	arg1	forms					1854:1858	commonly used recombinant forms	1828:1858	commonly used recombinant forms	1828:1858	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	6	43	theme	IgG1	1410:1413	arg1	Fc					1415:1416	IgG1 Fc	1410:1416	IgG1 Fc	1410:1416	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	1	44	theme	cytotoxic	259:267	arg1	response					269:276	a protective cytotoxic response	246:276	a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue	246:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	6	45	contain	having	1463:1468	arg2	N-glycans					1513:1521	primarily complex-type and highly branched N-glycans	1470:1521	primarily complex-type and highly branched N-glycans	1470:1521	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	6	45	contain	having	1463:1468	arg1	CD16a					1457:1461	CD16a	1457:1461	CD16a having primarily complex-type and highly branched N-glycans	1457:1521	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	7	46	theme	glycan	1639:1644	arg1	composition					1646:1656	CD16a glycan composition	1633:1656	CD16a glycan composition	1633:1656	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	8	47	from	forms	1854:1858	arg1	distinct					1814:1821	distinct	1814:1821	distinct	1814:1821	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	5	48	theme	soluble	1271:1277	arg1	CD16a					1279:1283	recombinant soluble CD16a	1259:1283	recombinant soluble CD16a	1259:1283	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	0	49	theme	γ	34:34	arg1	N-glycans					50:58	CD16a/Fc γ receptor IIIa N-glycans	25:58	CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	25:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	50	from	γ-chain	1098:1104	arg1	cells					1117:1121	HEK293F cells	1109:1121	HEK293F cells	1109:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	6	51	dep	affinity	1439:1446	arg1	did					1453:1455	did	1453:1455	did	1453:1455	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	0	52	theme	IIIa	45:48	arg1	N-glycans					50:58	CD16a/Fc γ receptor IIIa N-glycans	25:58	CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	25:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	6	53	dep	did	1453:1455	arg1	than					1448:1451	than	1448:1451	than	1448:1451	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	3	54	theme	primary	668:674	arg1	cells					679:683	the primary NK cells	664:683	the primary NK cells of three donors	664:699	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	1	55	theme	natural	206:212	arg1	NK					222:223	NK	222:223	NK	222:223	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	55	theme	natural	206:212	arg1	killer					214:219	natural killer	206:219	human natural killer (NK) cells	200:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	7	56	gly	glycoforms	1605:1614	arg1	CD16a					1599:1603	the two CD16a glycoforms	1591:1614	the two CD16a glycoforms	1591:1614	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	6	57	theme	oligomannose	1381:1392	arg1	N-glycans					1394:1402	oligomannose N-glycans	1381:1402	oligomannose N-glycans	1381:1402	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	5	58	theme	recombinant	963:973	arg1	CD16a					975:979	Tethering recombinant CD16a	953:979	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells	953:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	4	59	dep	those	860:864	arg1	%					949:949	82%	947:949	82%	947:949	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	5	60	theme	similar	1160:1166	arg1	N-glycoforms					1147:1158	N-glycoforms	1147:1158	N-glycoforms similar to NK cell-derived CD16a	1147:1191	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	4	61	theme	N-glycan	824:831	arg1	processing					833:842	restricted N-glycan processing	813:842	restricted N-glycan processing	813:842	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	6	62	theme	greater	1431:1437	arg1	affinity					1439:1446	12-fold greater affinity	1423:1446	12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans	1423:1521	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	2	63	with	antibodies	395:404	arg1	affinity					438:445	greater Fc-mediated affinity	418:445	greater Fc-mediated affinity for CD16a	418:455	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	7	64	gly	glycoprotein	1675:1686	arg1	glycoprotein					1675:1686	the glycoprotein's	1671:1688	the glycoprotein's structure	1671:1698	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	9	65	theme	cell	1916:1919	arg1	lineage					1921:1927	cell lineage	1916:1927	cell lineage	1916:1927	Furthermore, our study provides critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity.					
29330305	8	66	from	cells	1758:1762	arg1	CD16a					1730:1734	CD16a	1730:1734	CD16a from primary human NK cells	1730:1762	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	6	67	theme	antibody	1352:1359	arg1	binding					1361:1367	antibody binding	1352:1367	antibody binding	1352:1367	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	1	68	theme	antibody	166:173	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	68	theme	antibody	166:173	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	3	69	theme	hybrid	738:743	arg1	%					781:781	23%	779:781	23%	779:781	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	3	69	theme	hybrid	738:743	arg1	proportion					724:733	a large proportion	716:733	a large proportion of hybrid (22%) and oligomannose N-glycans (23%)	716:782	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	6	70	theme	branched	1504:1511	arg1	N-glycans					1513:1521	primarily complex-type and highly branched N-glycans	1470:1521	primarily complex-type and highly branched N-glycans	1470:1521	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	4	71	theme	recombinant	873:883	arg1	forms					891:895	the recombinant CD16a forms	869:895	the recombinant CD16a forms	869:895	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	2	72	link	asparagine-linked	571:587	arg1	N-glycans					604:612	N-glycans	604:612	N-glycans	604:612	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	72	link	asparagine-linked	571:587	arg1	carbohydrates					589:601	the asparagine-linked carbohydrates	567:601	the asparagine-linked carbohydrates (N-glycans)	567:613	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	72	link	asparagine-linked	571:587	arg1	factor					517:522	one significant factor	501:522	one significant factor that promotes antibody-CD16a interactions	501:564	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	1	73	theme	tissue	364:369	arg1	surface					330:336	the surface	326:336	the surface of a pathogen or diseased tissue	326:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	7	74	theme	CD16a	1599:1603	arg1	glycoforms					1605:1614	the two CD16a glycoforms	1591:1614	the two CD16a glycoforms	1591:1614	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	8	75	theme	human	1749:1753	arg1	cells					1758:1762	primary human NK cells	1741:1762	primary human NK cells	1741:1762	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	4	76	theme	CD16a	885:889	arg1	forms					891:895	the recombinant CD16a forms	869:895	the recombinant CD16a forms	869:895	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	1	77	theme	CD16a/Fc	120:127	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	77	theme	CD16a/Fc	120:127	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	5	78	theme	recombinant	1259:1269	arg1	CD16a					1279:1283	recombinant soluble CD16a	1259:1283	recombinant soluble CD16a	1259:1283	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	0	79	theme	primary	65:71	arg1	cells					82:86	primary human NK cells	65:86	primary human NK cells	65:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	80	theme	transmembrane	1014:1026	arg1	domains					1046:1052	the transmembrane and intracellular domains	1010:1052	the transmembrane and intracellular domains	1010:1052	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	1	81	theme	receptor	131:138	arg1	IIIa					140:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa	120:143	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	1	81	theme	receptor	131:138	arg1	receptor					178:185	the most abundant antibody Fc receptor	148:185	the most abundant antibody Fc receptor expressed on human natural killer (NK) cells	148:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	5	82	theme	different	1218:1226	arg1	N-glycoforms					1205:1216	N-glycoforms	1205:1216	N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a	1205:1283	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	3	83	theme	oligomannose	755:766	arg1	N-glycans					768:776	hybrid (22%) and oligomannose N-glycans	738:776	N-glycans	768:776	Here, we purified CD16a from the primary NK cells of three donors and identified a large proportion of hybrid (22%) and oligomannose N-glycans (23%).					
29330305	0	84	theme	NK	79:80	arg1	cells					82:86	primary human NK cells	65:86	primary human NK cells	65:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	85	theme	ϵ	1087:1087	arg1	γ-chain					1098:1104	the Fc ϵ receptor γ-chain	1080:1104	the Fc ϵ receptor γ-chain in HEK293F cells	1080:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	2	86	theme	superior	462:469	arg1	outcome					483:489	superior therapeutic outcome	462:489	superior therapeutic outcome	462:489	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	0	87	from	processing	11:20	arg1	cells					82:86	primary human NK cells	65:86	primary human NK cells	65:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	7	88	from	changes	1565:1571	arg1	spectra					1580:1586	NMR spectra	1576:1586	NMR spectra of the two CD16a glycoforms	1576:1614	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	7	89	theme	binding	1539:1545	arg1	activity					1547:1554	binding activity	1539:1554	binding activity	1539:1554	The changes in binding activity mirrored changes in NMR spectra of the two CD16a glycoforms, indicating that CD16a glycan composition also affects the glycoprotein's structure.					
29330305	9	90	theme	N-glycan	1946:1953	arg1	composition					1955:1965	CD16a N-glycan composition	1940:1965	CD16a N-glycan composition	1940:1965	Furthermore, our study provides critical evidence that cell lineage determines CD16a N-glycan composition and antibody-binding affinity.					
29330305	6	91	theme	N-glycan	1322:1329	arg1	composition					1331:1341	CD16a N-glycan composition	1316:1341	CD16a N-glycan composition	1316:1341	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	2	92	theme	greater	418:424	arg1	affinity					438:445	greater Fc-mediated affinity	418:445	greater Fc-mediated affinity for CD16a	418:455	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	5	93	theme	intracellular	1032:1044	arg1	domains					1046:1052	the transmembrane and intracellular domains	1010:1052	the transmembrane and intracellular domains	1010:1052	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	0	94	theme	CD16a/Fc	25:32	arg1	N-glycans					50:58	CD16a/Fc γ receptor IIIa N-glycans	25:58	CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	25:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	1	95	theme	protective	248:257	arg1	response					269:276	a protective cytotoxic response	246:276	a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue	246:369	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	8	96	theme	used	1837:1840	arg1	forms					1854:1858	commonly used recombinant forms	1828:1858	commonly used recombinant forms	1828:1858	These results indicated that CD16a from primary human NK cells is compositionally, and likely also functionally, distinct from commonly used recombinant forms.					
29330305	0	97	theme	receptor	36:43	arg1	N-glycans					50:58	CD16a/Fc γ receptor IIIa N-glycans	25:58	CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	25:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	6	98	from	differences	1301:1311	arg1	composition					1331:1341	CD16a N-glycan composition	1316:1341	CD16a N-glycan composition	1316:1341	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	2	99	theme	monoclonal	384:393	arg1	antibodies					395:404	Therapeutic monoclonal antibodies	372:404	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a	372:455	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	2	99	theme	monoclonal	384:393	arg1	mAbs					407:410	mAbs	407:410	mAbs	407:410	Therapeutic monoclonal antibodies (mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome; however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates (N-glycans), remains undefined.					
29330305	0	100	theme	N-glycans	50:58	arg1	processing					11:20	Restricted processing	0:20	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells	0:86	Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function.					
29330305	5	101	theme	HEK293F	1109:1115	arg1	cells					1117:1121	HEK293F cells	1109:1121	HEK293F cells	1109:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
29330305	6	102	theme	complex-type	1480:1491	arg1	N-glycans					1513:1521	primarily complex-type and highly branched N-glycans	1470:1521	primarily complex-type and highly branched N-glycans	1470:1521	Of note, these differences in CD16a N-glycan composition affected antibody binding: CD16a with oligomannose N-glycans bound IgG1 Fc with 12-fold greater affinity than did CD16a having primarily complex-type and highly branched N-glycans.					
29330305	4	103	contain	have	904:907	arg1	forms					891:895	the recombinant CD16a forms	869:895	the recombinant CD16a forms	869:895	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	4	103	contain	have	904:907	arg2	N-glycans					936:944	predominantly complex-type N-glycans	909:944	predominantly complex-type N-glycans	909:944	These proportions indicated restricted N-glycan processing and were unlike those of the recombinant CD16a forms, which have predominantly complex-type N-glycans (82%).					
29330305	1	104	theme	killer	214:219	arg1	cells					226:230	human natural killer (NK) cells	200:230	human natural killer (NK) cells	200:230	CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer (NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue.					
29330305	5	105	theme	Tethering	953:961	arg1	CD16a					975:979	Tethering recombinant CD16a	953:979	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells	953:1121	Tethering recombinant CD16a to the membrane by including the transmembrane and intracellular domains and via coexpression with the Fc ϵ receptor γ-chain in HEK293F cells was expected to produce N-glycoforms similar to NK cell-derived CD16a but yielded N-glycoforms different from NK cell-derived CD16a and recombinant soluble CD16a.					
31501225	6	0	theme	LC	1096:1097	arg1	patients					1099:1106	HBV-related LC patients	1084:1106	HBV-related LC patients	1084:1106	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	4	1	theme	18O/16O	596:602	arg1	labeling					615:622	18O/16O C-terminal labeling	596:622	18O/16O C-terminal labeling	596:622	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	9	2	theme	unique	1385:1390	arg1	signature					1399:1407	a unique glycan signature	1383:1407	a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases	1383:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	3	theme	signature	1399:1407	arg1	part					1375:1378	part	1375:1378	part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases	1375:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	3	theme	signature	1399:1407	arg1	N-glycopeptides					1350:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	4	4	theme	HBV-related	677:687	arg1	HCC					715:717	HCC	715:717	HCC	715:717	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	4	theme	HBV-related	677:687	arg1	carcinoma					704:712	HBV-related hepatocellular carcinoma	677:712	HBV-related hepatocellular carcinoma (HCC)	677:718	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	5	used	used	557:560	arg2	We					554:555	We	554:555	We	554:555	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	2	6	theme	molecular	304:312	arg1	pathogenesis					314:325	the molecular pathogenesis	300:325	the molecular pathogenesis of liver damage and cancer	300:352	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	0	7	theme	Hepatitis	62:70	arg1	Diseases					94:101	Hepatitis B Virus-related Liver Diseases	62:101	Hepatitis B Virus-related Liver Diseases	62:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	2	8	theme	cancer	347:352	arg1	pathogenesis					314:325	the molecular pathogenesis	300:325	the molecular pathogenesis of liver damage and cancer	300:352	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	4	9	from	patients	663:670	arg1	serum					652:656	serum	652:656	serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC)	652:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	9	from	patients	663:670	arg1	comparisons					637:647	82 comparisons	634:647	82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC)	634:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	10	theme	serum	652:656	arg1	comparisons					637:647	82 comparisons	634:647	82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC)	634:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	6	11	theme	HBV-related	1084:1094	arg1	patients					1099:1106	HBV-related LC patients	1084:1106	HBV-related LC patients	1084:1106	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	2	12	theme	specific	242:249	arg1	information					256:266	specific site information	242:266	specific site information	242:266	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	9	13	dep	signature	1399:1407	arg1	providing					1450:1458	providing	1450:1458	providing potential diagnostic clues in HBV-related liver diseases	1450:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	13	dep	signature	1399:1407	arg1	indicating					1409:1418	indicating	1409:1418	indicating an IgA-mediated mechanism	1409:1444	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	14	theme	liver	1502:1506	arg1	diseases					1508:1515	HBV-related liver diseases	1490:1515	HBV-related liver diseases	1490:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	3	15	theme	unbiased	361:368	arg1	quantification					370:383	unbiased quantification	361:383	unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	361:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	7	16	theme	HCC	1214:1216	arg1	patients					1218:1225	HBV-related HCC patients	1202:1225	HBV-related HCC patients	1202:1225	In contrast, the two glycopeptides of IgA2 fell back down in HBV-related HCC patients.					
31501225	2	17	theme	better	276:281	arg1	understanding					283:295	better understanding	276:295	better understanding of the molecular pathogenesis of liver damage and cancer	276:352	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	5	18	theme	donor-HBV-LC-HCC	887:902	arg1	cascade					904:910	the healthy donor-HBV-LC-HCC cascade	875:910	the healthy donor-HBV-LC-HCC cascade	875:910	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	0	19	theme	B	72:72	arg1	Diseases					94:101	Hepatitis B Virus-related Liver Diseases	62:101	Hepatitis B Virus-related Liver Diseases	62:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	2	20	gly	N-glycopeptides	183:197	arg2	N-glycopeptides					183:197	N-glycopeptides	183:197	N-glycopeptides that correspond to N-glycan structure with specific site information	183:266	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	0	21	theme	Liver	88:92	arg1	Diseases					94:101	Hepatitis B Virus-related Liver Diseases	62:101	Hepatitis B Virus-related Liver Diseases	62:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	8	22	gly	glycopeptides	1279:1291	arg2	glycopeptides					1279:1291	two glycopeptides	1275:1291	two glycopeptides	1275:1291	In addition, the variation in the abundance of two glycopeptides was not caused by its protein concentration.					
31501225	3	23	theme	40-kDa	473:478	arg1	weight					465:470	40-55 kDa molecular weight	445:470	40-55 kDa molecular weight (40-kDa band)	445:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	23	theme	40-kDa	473:478	arg1	band					480:483	40-kDa band	473:483	40-kDa band	473:483	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	7	24	theme	IgA2	1179:1182	arg1	glycopeptides					1162:1174	the two glycopeptides	1154:1174	the two glycopeptides of IgA2	1154:1182	In contrast, the two glycopeptides of IgA2 fell back down in HBV-related HCC patients.					
31501225	3	25	theme	serum	420:424	arg1	glycoproteins					426:438	serum glycoproteins	420:438	serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	420:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	4	26	from	comparisons	637:647	arg1	patients					663:670	patients	663:670	patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC)	663:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	0	27	theme	N-glycopeptide	0:13	arg1	Signatures					15:24	N-glycopeptide Signatures	0:24	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.	0:102	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	3	28	theme	cluster	409:415	arg1	quantification					370:383	unbiased quantification	361:383	unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	361:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	9	29	gly	N-glycopeptides	1350:1364	arg2	N-glycopeptides					1350:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	29	gly	N-glycopeptides	1350:1364	arg2	part					1375:1378	part	1375:1378	part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases	1375:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	5	30	theme	relative	828:835	arg1	N-glycopeptide					813:826	N-glycopeptide	813:826	N-glycopeptide relative to the protein content	813:858	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	1	31	theme	N-glycosylation	104:118	arg1	alteration					120:129	N-glycosylation alteration	104:129	N-glycosylation alteration	104:129	N-glycosylation alteration has been reported in liver diseases.					
31501225	6	32	theme	HBV	1051:1053	arg1	infection					1055:1063	HBV infection	1051:1063	HBV infection	1051:1063	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	3	33	theme	kDa	451:453	arg1	weight					465:470	40-55 kDa molecular weight	445:470	40-55 kDa molecular weight (40-kDa band)	445:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	33	theme	kDa	451:453	arg1	band					480:483	40-kDa band	473:483	40-kDa band	473:483	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	5	34	theme	reaction	761:768	arg1	MRM					782:784	MRM	782:784	MRM	782:784	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	5	34	theme	reaction	761:768	arg1	monitoring					770:779	multiple reaction monitoring	752:779	multiple reaction monitoring (MRM)	752:785	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	9	35	theme	altered	1342:1348	arg1	part					1375:1378	part	1375:1378	part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases	1375:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	35	theme	altered	1342:1348	arg1	N-glycopeptides					1350:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides	1338:1364	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	36	dep	potential	1460:1468	arg1	diagnostic					1470:1479	diagnostic	1470:1479	diagnostic	1470:1479	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	3	37	theme	hepatitis	506:514	arg1	virus					518:522	hepatitis B virus	506:522	hepatitis B virus (HBV)	506:528	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	37	theme	hepatitis	506:514	arg1	HBV					525:527	HBV	525:527	HBV	525:527	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	1	38	theme	liver	152:156	arg1	diseases					158:165	liver diseases	152:165	liver diseases	152:165	N-glycosylation alteration has been reported in liver diseases.					
31501225	3	39	theme	-related	529:536	arg1	diseases					544:551	hepatitis B virus (HBV)-related liver diseases	506:551	hepatitis B virus (HBV)-related liver diseases	506:551	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	2	40	with	structure	227:235	arg1	information					256:266	specific site information	242:266	specific site information	242:266	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	6	41	from	patients	1037:1044	arg1	serum					1026:1030	serum	1026:1030	serum from patients with HBV infection	1026:1063	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	2	42	theme	liver	330:334	arg1	damage					336:341	liver damage	330:341	liver damage	330:341	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	9	43	theme	glycan	1392:1397	arg1	signature					1399:1407	a unique glycan signature	1383:1407	a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases	1383:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	4	44	theme	C-terminal	604:613	arg1	labeling					615:622	18O/16O C-terminal labeling	596:622	18O/16O C-terminal labeling	596:622	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	2	45	theme	pathogenesis	314:325	arg1	understanding					283:295	better understanding	276:295	better understanding of the molecular pathogenesis of liver damage and cancer	276:352	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	6	46	gly	glycopeptides	973:985	arg2	glycopeptides					973:985	the glycopeptides	969:985	the glycopeptides of IgA2	969:993	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	6	46	gly	glycopeptides	973:985	arg1	IgA2					990:993	IgA2	990:993	IgA2	990:993	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	9	47	theme	IgA-mediated	1423:1434	arg1	mechanism					1436:1444	an IgA-mediated mechanism	1420:1444	an IgA-mediated mechanism	1420:1444	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	9	48	from	clues	1481:1485	arg1	diseases					1508:1515	HBV-related liver diseases	1490:1515	HBV-related liver diseases	1490:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	4	49	theme	hepatocellular	689:702	arg1	HCC					715:717	HCC	715:717	HCC	715:717	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	49	theme	hepatocellular	689:702	arg1	carcinoma					704:712	HBV-related hepatocellular carcinoma	677:712	HBV-related hepatocellular carcinoma (HCC)	677:718	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	3	50	with	glycoproteins	426:438	arg1	weight					465:470	40-55 kDa molecular weight	445:470	40-55 kDa molecular weight (40-kDa band)	445:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	50	with	glycoproteins	426:438	arg1	band					480:483	40-kDa band	473:483	40-kDa band	473:483	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	9	51	theme	potential	1460:1468	arg1	clues					1481:1485	potential diagnostic clues	1460:1485	potential diagnostic clues in HBV-related liver diseases	1460:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	2	52	theme	site	251:254	arg1	information					256:266	specific site information	242:266	specific site information	242:266	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	4	53	gly	N-glycopeptide	565:578	arg2	N-glycopeptide					565:578	N-glycopeptide	565:578	N-glycopeptide	565:578	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	7	54	theme	HBV-related	1202:1212	arg1	patients					1218:1225	HBV-related HCC patients	1202:1225	HBV-related HCC patients	1202:1225	In contrast, the two glycopeptides of IgA2 fell back down in HBV-related HCC patients.					
31501225	4	55	theme	liver	724:728	arg1	cirrhosis					730:738	liver cirrhosis	724:738	liver cirrhosis (LC)	724:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	55	theme	liver	724:728	arg1	LC					741:742	LC	741:742	LC	741:742	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	7	56	gly	glycopeptides	1162:1174	arg2	glycopeptides					1162:1174	the two glycopeptides	1154:1174	the two glycopeptides of IgA2	1154:1182	In contrast, the two glycopeptides of IgA2 fell back down in HBV-related HCC patients.					
31501225	7	56	gly	glycopeptides	1162:1174	arg1	IgA2					1179:1182	IgA2	1179:1182	IgA2	1179:1182	In contrast, the two glycopeptides of IgA2 fell back down in HBV-related HCC patients.					
31501225	9	57	theme	HBV-related	1490:1500	arg1	diseases					1508:1515	HBV-related liver diseases	1490:1515	HBV-related liver diseases	1490:1515	The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.					
31501225	5	58	theme	multiple	752:759	arg1	MRM					782:784	MRM	782:784	MRM	782:784	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	5	58	theme	multiple	752:759	arg1	monitoring					770:779	multiple reaction monitoring	752:779	multiple reaction monitoring (MRM)	752:785	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	8	59	from	variation	1245:1253	arg1	abundance					1262:1270	the abundance	1258:1270	the abundance of two glycopeptides	1258:1291	In addition, the variation in the abundance of two glycopeptides was not caused by its protein concentration.					
31501225	5	60	theme	healthy	879:885	arg1	cascade					904:910	the healthy donor-HBV-LC-HCC cascade	875:910	the healthy donor-HBV-LC-HCC cascade	875:910	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	8	61	theme	glycopeptides	1279:1291	arg1	abundance					1262:1270	the abundance	1258:1270	the abundance of two glycopeptides	1258:1291	In addition, the variation in the abundance of two glycopeptides was not caused by its protein concentration.					
31501225	0	62	theme	Virus-related	74:86	arg1	Diseases					94:101	Hepatitis B Virus-related Liver Diseases	62:101	Hepatitis B Virus-related Liver Diseases	62:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	0	63	from	Signatures	15:24	arg1	Serum					37:41	Serum	37:41	Serum from Patients with Hepatitis B Virus-related Liver Diseases	37:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	0	63	from	Signatures	15:24	arg1	Patients					48:55	Patients	48:55	Patients with Hepatitis B Virus-related Liver Diseases	48:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	2	64	theme	damage	336:341	arg1	pathogenesis					314:325	the molecular pathogenesis	300:325	the molecular pathogenesis of liver damage and cancer	300:352	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	6	65	theme	IgA2	990:993	arg1	glycopeptides					973:985	the glycopeptides	969:985	the glycopeptides of IgA2	969:993	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	0	66	from	Patients	48:55	arg1	Serum					37:41	Serum	37:41	Serum from Patients with Hepatitis B Virus-related Liver Diseases	37:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	0	66	from	Patients	48:55	arg1	Signatures					15:24	N-glycopeptide Signatures	0:24	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.	0:102	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	3	67	gly	glycoproteins	426:438	arg1	glycoproteins					426:438	serum glycoproteins	420:438	serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	420:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	68	theme	N-glycopeptides	388:402	arg1	quantification					370:383	unbiased quantification	361:383	unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	361:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	8	69	theme	protein	1315:1321	arg1	concentration					1323:1335	its protein concentration	1311:1335	its protein concentration	1311:1335	In addition, the variation in the abundance of two glycopeptides was not caused by its protein concentration.					
31501225	3	70	theme	glycoproteins	426:438	arg1	cluster					409:415	a cluster	407:415	a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band)	407:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	4	71	theme	N-glycopeptide	565:578	arg1	method					580:585	an N-glycopeptide method	562:585	an N-glycopeptide method based on 18O/16O C-terminal labeling	562:622	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	0	72	theme	IgA2	29:32	arg1	Signatures					15:24	N-glycopeptide Signatures	0:24	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.	0:102	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	3	73	gly	N-glycopeptides	388:402	arg2	N-glycopeptides					388:402	N-glycopeptides	388:402	N-glycopeptides	388:402	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	5	74	theme	protein	844:850	arg1	content					852:858	the protein content	840:858	the protein content	840:858	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	0	75	gly	N-glycopeptide	0:13	arg2	N-glycopeptide					0:13	N-glycopeptide Signatures	0:24	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.	0:102	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	3	76	theme	molecular	455:463	arg1	weight					465:470	40-55 kDa molecular weight	445:470	40-55 kDa molecular weight (40-kDa band)	445:484	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	76	theme	molecular	455:463	arg1	band					480:483	40-kDa band	473:483	40-kDa band	473:483	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	6	77	theme	healthy	1126:1132	arg1	donor					1134:1138	healthy donor	1126:1138	healthy donor	1126:1138	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	3	78	theme	40-55	445:449	arg1	kDa					451:453	kDa	451:453	kDa	451:453	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	4	79	with	patients	663:670	arg1	cirrhosis					730:738	liver cirrhosis	724:738	liver cirrhosis (LC)	724:743	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	79	with	patients	663:670	arg1	LC					741:742	LC	741:742	LC	741:742	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	79	with	patients	663:670	arg1	HCC					715:717	HCC	715:717	HCC	715:717	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	4	79	with	patients	663:670	arg1	carcinoma					704:712	HBV-related hepatocellular carcinoma	677:712	HBV-related hepatocellular carcinoma (HCC)	677:718	We used an N-glycopeptide method based on 18O/16O C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC).					
31501225	5	80	gly	N-glycopeptide	813:826	arg2	N-glycopeptide					813:826	N-glycopeptide	813:826	N-glycopeptide relative to the protein content	813:858	Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade.					
31501225	6	81	with	patients	1037:1044	arg1	infection					1055:1063	HBV infection	1051:1063	HBV infection	1051:1063	TPLTAN205ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA2 were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor.					
31501225	2	82	theme	N-glycan	218:225	arg1	structure					227:235	N-glycan structure	218:235	N-glycan structure with specific site information	218:266	Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer.					
31501225	0	83	with	Patients	48:55	arg1	Diseases					94:101	Hepatitis B Virus-related Liver Diseases	62:101	Hepatitis B Virus-related Liver Diseases	62:101	N-glycopeptide Signatures of IgA2 in Serum from Patients with Hepatitis B Virus-related Liver Diseases.					
31501225	3	84	theme	B	516:516	arg1	virus					518:522	hepatitis B virus	506:522	hepatitis B virus (HBV)	506:528	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	84	theme	B	516:516	arg1	HBV					525:527	HBV	525:527	HBV	525:527	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
31501225	3	85	theme	liver	538:542	arg1	diseases					544:551	hepatitis B virus (HBV)-related liver diseases	506:551	hepatitis B virus (HBV)-related liver diseases	506:551	Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases.					
30343578	0	0	theme	Lines	80:84	arg1	N-Glycomes					44:53	Cell-Surface N-Glycomes	31:53	Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines	31:84	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	2	1	theme	normal	487:492	arg1	cells					504:508	the normal liver LO2 cells	483:508	the normal liver LO2 cells	483:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	1	2	theme	intracellular	150:162	arg1	development					203:213	development	203:213	development	203:213	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	2	theme	intracellular	150:162	arg1	recognition					221:231	cell recognition	216:231	cell recognition	216:231	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	2	theme	intracellular	150:162	arg1	adhesion					190:197	cell adhesion	185:197	cell adhesion	185:197	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	2	theme	intracellular	150:162	arg1	development					252:262	cancer development	245:262	cancer development	245:262	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	2	theme	intracellular	150:162	arg1	processes					164:172	both inter- and intracellular processes	134:172	both inter- and intracellular processes	134:172	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	5	3	with	N-glycans	1111:1119	arg1	structures					1142:1151	tetra-antennary structures	1126:1151	tetra-antennary structures	1126:1151	The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells.					
30343578	5	4	theme	HepG2	1188:1192	arg1	cells					1194:1198	the HepG2 cells	1184:1198	the HepG2 cells	1184:1198	The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells.					
30343578	0	5	theme	Cell	75:78	arg1	Lines					80:84	LO2 Cell Lines	71:84	LO2 Cell Lines	71:84	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	5	6	theme	hybrid	1104:1109	arg1	N-glycans					1111:1119	hybrid N-glycans	1104:1119	hybrid N-glycans with tetra-antennary structures	1104:1151	The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells.					
30343578	1	7	theme	cancer	245:250	arg1	development					252:262	cancer development	245:262	cancer development	245:262	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	6	8	theme	markers	1376:1382	arg1	study					1353:1357	future validation study	1335:1357	future validation study of glycosylation markers in HCC	1335:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	5	9	theme	N-glycans	1111:1119	arg1	percentage					1090:1099	The percentage	1086:1099	The percentage of hybrid N-glycans with tetra-antennary structures	1086:1151	The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells.					
30343578	4	10	theme	spectrum-level	820:833	arg1	FDR					835:837	spectrum-level FDR	820:837	spectrum-level FDR no bigger than 1%	820:855	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	6	11	theme	glycosylation	1362:1374	arg1	markers					1376:1382	glycosylation markers	1362:1382	glycosylation markers in HCC	1362:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	12	theme	cell-surface	722:733	arg1	N-glycomes					735:744	cell-surface N-glycomes	722:744	cell-surface N-glycomes of the HepG2 and LO2 cells	722:771	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	13	theme	proteins	548:555	arg1	digestion					535:543	sequential trypsin digestion	516:543	sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans	516:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	14	gly	N-glycopeptides	629:643	arg2	N-glycopeptides					629:643	N-glycopeptides	629:643	N-glycopeptides	629:643	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	6	15	from	study	1353:1357	arg1	HCC					1387:1389	HCC	1387:1389	HCC	1387:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	16	theme	N-glycans	1277:1285	arg1	study					1230:1234	This comprehensive discovery study	1201:1234	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2	1201:1301	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	16	theme	N-glycans	1277:1285	arg1	reference					1321:1329	a solid reference	1313:1329	a solid reference for future validation study of glycosylation markers in HCC	1313:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	17	theme	trypsin	527:533	arg1	digestion					535:543	sequential trypsin digestion	516:543	sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans	516:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	2	18	theme	N-glycomics	386:396	arg1	study					398:402	our comparative N-glycomics study	370:402	our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	370:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	2	19	theme	cells	504:508	arg1	N-glycans					420:428	cell-surface N-glycans	407:428	cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	407:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	3	20	theme	N-glycans	711:719	arg1	permethylation					680:693	CH3I permethylation	675:693	CH3I permethylation of the enriched N-glycans	675:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	20	theme	N-glycans	711:719	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	6	21	theme	cell-surface	1264:1275	arg1	N-glycans					1277:1285	differentially expressed cell-surface N-glycans	1239:1285	differentially expressed cell-surface N-glycans	1239:1285	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	0	22	theme	Glycomics	12:20	arg1	Study					22:26	Comparative Glycomics Study	0:26	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.	0:85	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	4	23	theme	structural	948:957	arg1	information					959:969	comprehensive structural information	934:969	comprehensive structural information (not only monosaccharide composition, but also sequence and linkage)	934:1038	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	6	24	theme	expressed	1254:1262	arg1	N-glycans					1277:1285	differentially expressed cell-surface N-glycans	1239:1285	differentially expressed cell-surface N-glycans	1239:1285	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	2	25	theme	LO2	500:502	arg1	cells					504:508	the normal liver LO2 cells	483:508	the normal liver LO2 cells	483:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	1	26	theme	cell	185:188	arg1	adhesion					190:197	cell adhesion	185:197	cell adhesion	185:197	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	0	27	theme	Comparative	0:10	arg1	Study					22:26	Comparative Glycomics Study	0:26	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.	0:85	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	3	28	theme	HepG2	753:757	arg1	cells					767:771	the HepG2 and LO2 cells	749:771	cells	767:771	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	29	theme	N-glycans	664:672	arg1	enrichment					650:659	N-glycopeptides, PGC enrichment	629:659	enrichment	650:659	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	4	30	theme	monosaccharide	981:994	arg1	composition					996:1006	monosaccharide composition	981:1006	monosaccharide composition	981:1006	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	1	31	theme	Cell-surface	87:98	arg1	N-glycans					100:108	Cell-surface N-glycans	87:108	Cell-surface N-glycans	87:108	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	2	32	theme	comparative	374:384	arg1	study					398:402	our comparative N-glycomics study	370:402	our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	370:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	2	33	theme	carcinoma	452:460	arg1	cells					474:478	the hepatocellular carcinoma (HCC) HepG2 cells	433:478	the hepatocellular carcinoma (HCC) HepG2 cells	433:478	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	6	34	theme	validation	1342:1351	arg1	study					1353:1357	future validation study	1335:1357	future validation study of glycosylation markers in HCC	1335:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	4	35	theme	engine	1068:1073	arg1	GlySeeker					1075:1083	N-glycan database search engine GlySeeker	1043:1083	N-glycan database search engine GlySeeker	1043:1083	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	2	36	theme	hepatocellular	437:450	arg1	HCC					463:465	HCC	463:465	HCC	463:465	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	2	36	theme	hepatocellular	437:450	arg1	carcinoma					452:460	hepatocellular carcinoma	437:460	the hepatocellular carcinoma (HCC) HepG2 cells	433:478	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	1	37	theme	detailed	280:287	arg1	characterization					300:315	detailed structural characterization	280:315	detailed structural characterization of these N-glycans	280:334	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	6	38	theme	future	1335:1340	arg1	study					1353:1357	future validation study	1335:1357	future validation study of glycosylation markers in HCC	1335:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	39	theme	C18	558:560	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	39	theme	C18	558:560	arg1	depletion					562:570	C18 depletion	558:570	C18 depletion of peptides without glycosylation	558:604	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	2	40	theme	HepG2	468:472	arg1	cells					474:478	the hepatocellular carcinoma (HCC) HepG2 cells	433:478	the hepatocellular carcinoma (HCC) HepG2 cells	433:478	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	4	41	theme	comprehensive	934:946	arg1	information					959:969	comprehensive structural information	934:969	comprehensive structural information (not only monosaccharide composition, but also sequence and linkage)	934:1038	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	0	42	theme	N-Glycomes	44:53	arg1	Study					22:26	Comparative Glycomics Study	0:26	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.	0:85	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	1	43	theme	structural	289:298	arg1	characterization					300:315	detailed structural characterization	280:315	detailed structural characterization of these N-glycans	280:334	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	6	44	theme	discovery	1220:1228	arg1	study					1230:1234	This comprehensive discovery study	1201:1234	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2	1201:1301	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	44	theme	discovery	1220:1228	arg1	reference					1321:1329	a solid reference	1313:1329	a solid reference for future validation study of glycosylation markers in HCC	1313:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	45	theme	enrichment	650:659	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	45	theme	enrichment	650:659	arg1	digestion					616:624	PNGase F digestion	607:624	PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans	607:672	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	6	46	from	study	1230:1234	arg1	HepG2					1290:1294	HepG2	1290:1294	HepG2	1290:1294	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	46	from	study	1230:1234	arg1	LO2					1299:1301	LO2	1299:1301	LO2	1299:1301	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	2	47	theme	N-glycans	420:428	arg1	study					398:402	our comparative N-glycomics study	370:402	our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	370:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	0	48	theme	Cell-Surface	31:42	arg1	N-Glycomes					44:53	Cell-Surface N-Glycomes	31:53	Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines	31:84	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	2	49	theme	cells	474:478	arg1	N-glycans					420:428	cell-surface N-glycans	407:428	cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	407:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	3	50	theme	N-glycopeptides	629:643	arg1	enrichment					650:659	N-glycopeptides, PGC enrichment	629:659	enrichment	650:659	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	1	51	theme	cell	216:219	arg1	recognition					221:231	cell recognition	216:231	cell recognition	216:231	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	0	52	theme	HepG2	58:62	arg1	N-Glycomes					44:53	Cell-Surface N-Glycomes	31:53	Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines	31:84	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	5	53	theme	tetra-antennary	1126:1140	arg1	structures					1142:1151	tetra-antennary structures	1126:1151	tetra-antennary structures	1126:1151	The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells.					
30343578	4	54	dep	information	959:969	arg1	only					976:979	only	976:979	only	976:979	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	3	55	theme	peptides	575:582	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	55	theme	peptides	575:582	arg1	depletion					562:570	C18 depletion	558:570	C18 depletion of peptides without glycosylation	558:604	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	4	56	theme	bigger	842:847	arg1	FDR					835:837	spectrum-level FDR	820:837	spectrum-level FDR no bigger than 1%	820:855	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	1	57	theme	important	115:123	arg1	roles					125:129	important roles	115:129	important roles	115:129	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	3	58	theme	LO2	763:765	arg1	cells					767:771	the HepG2 and LO2 cells	749:771	cells	767:771	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	6	59	theme	comprehensive	1206:1218	arg1	study					1230:1234	This comprehensive discovery study	1201:1234	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2	1201:1301	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	59	theme	comprehensive	1206:1218	arg1	reference					1321:1329	a solid reference	1313:1329	a solid reference for future validation study of glycosylation markers in HCC	1313:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	4	60	theme	N-glycan	1043:1050	arg1	engine					1068:1073	N-glycan database search engine	1043:1073	N-glycan database search engine GlySeeker	1043:1083	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	1	61	theme	N-glycans	326:334	arg1	characterization					300:315	detailed structural characterization	280:315	detailed structural characterization of these N-glycans	280:334	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	3	62	theme	CH3I	675:678	arg1	permethylation					680:693	CH3I permethylation	675:693	CH3I permethylation of the enriched N-glycans	675:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	62	theme	CH3I	675:678	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	6	63	from	markers	1376:1382	arg1	HCC					1387:1389	HCC	1387:1389	HCC	1387:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	64	theme	cells	767:771	arg1	N-glycomes					735:744	cell-surface N-glycomes	722:744	cell-surface N-glycomes of the HepG2 and LO2 cells	722:771	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	4	65	theme	search	1061:1066	arg1	engine					1068:1073	N-glycan database search engine	1043:1073	N-glycan database search engine GlySeeker	1043:1083	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	0	66	theme	LO2	71:73	arg1	Lines					80:84	LO2 Cell Lines	71:84	LO2 Cell Lines	71:84	Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines.					
30343578	2	67	theme	cell-surface	407:418	arg1	N-glycans					420:428	cell-surface N-glycans	407:428	cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells	407:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	3	68	theme	PNGase	607:612	arg1	F					614:614	PNGase F	607:614	PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans	607:672	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	1	69	theme	inter-	139:144	arg1	development					203:213	development	203:213	development	203:213	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	69	theme	inter-	139:144	arg1	recognition					221:231	cell recognition	216:231	cell recognition	216:231	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	69	theme	inter-	139:144	arg1	adhesion					190:197	cell adhesion	185:197	cell adhesion	185:197	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	69	theme	inter-	139:144	arg1	development					252:262	cancer development	245:262	cancer development	245:262	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	1	69	theme	inter-	139:144	arg1	processes					164:172	both inter- and intracellular processes	134:172	both inter- and intracellular processes	134:172	Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount.					
30343578	4	70	theme	database	1052:1059	arg1	engine					1068:1073	N-glycan database search engine	1043:1073	N-glycan database search engine GlySeeker	1043:1083	With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker.					
30343578	6	71	theme	solid	1315:1319	arg1	study					1230:1234	This comprehensive discovery study	1201:1234	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2	1201:1301	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	6	71	theme	solid	1315:1319	arg1	reference					1321:1329	a solid reference	1313:1329	a solid reference for future validation study of glycosylation markers in HCC	1313:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	2	72	theme	liver	494:498	arg1	cells					504:508	the normal liver LO2 cells	483:508	the normal liver LO2 cells	483:508	Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells.					
30343578	6	73	from	HCC	1387:1389	arg1	study					1353:1357	future validation study	1335:1357	future validation study of glycosylation markers in HCC	1335:1389	This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC.					
30343578	3	74	theme	F	614:614	arg1	proteins					548:555	proteins	548:555	proteins	548:555	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	74	theme	F	614:614	arg1	digestion					616:624	PNGase F digestion	607:624	PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans	607:672	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	75	theme	enriched	702:709	arg1	N-glycans					711:719	the enriched N-glycans	698:719	the enriched N-glycans	698:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	76	theme	sequential	516:525	arg1	digestion					535:543	sequential trypsin digestion	516:543	sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans	516:719	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
30343578	3	77	dep	enrichment	650:659	arg1	PGC					646:648	N-glycopeptides, PGC enrichment	629:659	PGC	646:648	With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC-MS/MS (HCD).					
31611356	0	0	theme	Broadly	91:97	arg1	Antibodies					112:121	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	0	theme	Broadly	91:97	arg1	PGT126					134:139	PGT126	134:139	PGT126	134:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	0	theme	Broadly	91:97	arg1	PGT121					123:128	PGT121	123:128	PGT121	123:128	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	2	1	theme	neutralizing	447:458	arg1	antibodies					460:469	broadly neutralizing antibodies	439:469	broadly neutralizing antibodies (bNAbs)	439:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	2	1	theme	neutralizing	447:458	arg1	bNAbs					472:476	bNAbs	472:476	bNAbs	472:476	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	4	2	theme	glycan	784:789	arg1	absence					791:797	glycan absence	784:797	glycan absence at N332 or N448	784:813	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	7	3	dep	loss	1405:1408	arg1	event					1394:1398	event	1394:1398	event	1394:1398	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	3	dep	loss	1405:1408	arg1	the					1390:1392	the	1390:1392	the	1390:1392	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	1	4	theme	high-mannose	241:252	arg1	sites					277:281	high-mannose N-linked glycosylation sites	241:281	high-mannose N-linked glycosylation sites on HIV-1 gp120	241:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	0	5	theme	Complements	79:89	arg1	Antibodies					112:121	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	5	theme	Complements	79:89	arg1	PGT126					134:139	PGT126	134:139	PGT126	134:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	5	theme	Complements	79:89	arg1	PGT121					123:128	PGT121	123:128	PGT121	123:128	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	6	6	from	presence	1219:1226	arg1	N295					1248:1251	N295	1248:1251	N295	1248:1251	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	8	7	theme	additive	1584:1591	arg1	effects					1593:1599	additive effects	1584:1599	additive effects	1584:1599	Despite targeting the same region, Grft in combination with PGT121 and PGT126 produced additive effects.					
31611356	6	8	theme	C	1288:1288	arg1	strains					1290:1296	clade C strains	1282:1296	clade C strains	1282:1296	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	1	9	theme	N-linked	254:261	arg1	sites					277:281	high-mannose N-linked glycosylation sites	241:281	high-mannose N-linked glycosylation sites on HIV-1 gp120	241:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	5	10	theme	individual	1044:1053	arg1	glycans					1055:1061	individual glycans	1044:1061	individual glycans	1044:1061	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	6	11	theme	clade	1282:1286	arg1	strains					1290:1296	clade C strains	1282:1296	clade C strains	1282:1296	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	1	12	theme	glycosylation	263:275	arg1	sites					277:281	high-mannose N-linked glycosylation sites	241:281	high-mannose N-linked glycosylation sites on HIV-1 gp120	241:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	0	13	theme	Neutralizing	99:110	arg1	Antibodies					112:121	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	13	theme	Neutralizing	99:110	arg1	PGT126					134:139	PGT126	134:139	PGT126	134:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	13	theme	Neutralizing	99:110	arg1	PGT121					123:128	PGT121	123:128	PGT121	123:128	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	4	14	from	N295	778:781	arg1	removal					750:756	the removal	746:756	the removal of glycosylation at N295	746:781	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	3	15	theme	previous	495:502	arg1	work					504:507	previous work	495:507	previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120	495:689	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	7	16	theme	drastic	1472:1478	arg1	loss					1480:1483	a drastic loss	1470:1483	a drastic loss of potency	1470:1494	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	4	17	from	effects	842:848	arg1	potency					858:864	Grft potency	853:864	Grft potency	853:864	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	7	18	theme	potency	1488:1494	arg1	loss					1480:1483	a drastic loss	1470:1483	a drastic loss of potency	1470:1494	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	3	19	theme	glycosylation	663:675	arg1	site					677:680	nearly every glycosylation site	650:680	nearly every glycosylation site	650:680	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	4	20	theme	glycosylation	761:773	arg1	removal					750:756	the removal	746:756	the removal of glycosylation at N295	746:781	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	1	21	link	N-linked	254:261	arg1	sites					277:281	high-mannose N-linked glycosylation sites	241:281	high-mannose N-linked glycosylation sites on HIV-1 gp120	241:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	0	22	dep	Antibodies	112:121	arg1	Antibodies					112:121	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	22	dep	Antibodies	112:121	arg1	PGT126					134:139	PGT126	134:139	PGT126	134:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	22	dep	Antibodies	112:121	arg1	PGT121					123:128	PGT121	123:128	PGT121	123:128	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	8	23	from	Grft	1532:1535	arg1	combination					1540:1550	combination	1540:1550	combination with PGT121 and PGT126	1540:1573	Despite targeting the same region, Grft in combination with PGT121 and PGT126 produced additive effects.					
31611356	1	24	theme	antiviral	167:175	arg1	Griffithsin					142:152	Griffithsin	142:152	Griffithsin (Grft)	142:159	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	1	24	theme	antiviral	167:175	arg1	lectin					177:182	an antiviral lectin	164:182	an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120	164:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	4	25	theme	Grft	853:856	arg1	potency					858:864	Grft potency	853:864	Grft potency	853:864	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	0	26	from	Changes	44:50	arg1	Antibodies					112:121	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Complements Broadly Neutralizing Antibodies PGT121 and PGT126	79:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	26	from	Changes	44:50	arg1	Glycosylation					61:73	gp120 Glycosylation	55:73	gp120 Glycosylation	55:73	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	26	from	Changes	44:50	arg1	PGT121					123:128	PGT121	123:128	PGT121	123:128	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	0	26	from	Changes	44:50	arg1	PGT126					134:139	PGT126	134:139	PGT126	134:139	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	4	27	theme	Grft	729:732	arg1	activity					734:741	Grft activity	729:741	Grft activity	729:741	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	2	28	from	N295	349:352	arg1	glycosylation					332:344	glycosylation	332:344	glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	332:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	2	28	from	N295	349:352	arg1	factor					305:310	A key factor	299:310	A key factor for Grft potency	299:327	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	5	29	from	N448	987:990	arg1	at					984:985	the glycan at N448	973:990	the glycan at N448 shifting to N446	973:1007	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	8	30	with	combination	1540:1550	arg1	PGT121					1557:1562	PGT121	1557:1562	PGT121	1557:1562	Despite targeting the same region, Grft in combination with PGT121 and PGT126 produced additive effects.					
31611356	8	30	with	combination	1540:1550	arg1	PGT126					1568:1573	PGT126	1568:1573	PGT126	1568:1573	Despite targeting the same region, Grft in combination with PGT121 and PGT126 produced additive effects.					
31611356	7	31	theme	glycosylation	1424:1436	arg1	loss					1405:1408	a loss	1403:1408	a loss	1403:1408	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	31	theme	glycosylation	1424:1436	arg1	shift					1415:1419	a shift	1413:1419	a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency	1413:1494	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	32	dep	bNAbs	1363:1367	arg1	PGT126					1380:1385	PGT126	1380:1385	PGT126	1380:1385	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	32	dep	bNAbs	1363:1367	arg1	PGT121					1369:1374	PGT121	1369:1374	PGT121	1369:1374	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	32	dep	bNAbs	1363:1367	arg1	bNAbs					1363:1367	the bNAbs PGT121 and PGT126	1359:1385	the bNAbs PGT121 and PGT126	1359:1385	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	5	33	theme	glycan	977:982	arg1	N448					987:990	the glycan at N448	973:990	the glycan at N448 shifting to N446	973:1007	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	4	34	dep	loss	721:724	arg1	addition					695:702	addition	695:702	addition	695:702	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	2	35	theme	glycan	409:414	arg1	N332					394:397	N332	394:397	N332	394:397	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	2	35	theme	glycan	409:414	arg1	target					402:407	a target	400:407	a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	400:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	3	36	gly	glycosylation	663:675	arg2	site					677:680	nearly every glycosylation site	650:680	nearly every glycosylation site	650:680	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	7	37	from	N332	1441:1444	arg1	loss					1405:1408	a loss	1403:1408	a loss	1403:1408	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	7	37	from	N332	1441:1444	arg1	shift					1415:1419	a shift	1413:1419	a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency	1413:1494	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	4	38	contain	have	828:831	arg2	effects					842:848	moderate effects	833:848	moderate effects on Grft potency	833:864	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	4	38	contain	have	828:831	arg1	absence					791:797	glycan absence	784:797	glycan absence at N332 or N448	784:813	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	6	39	from	N295	1248:1251	arg1	glycosylation					1231:1243	glycosylation	1231:1243	glycosylation at N295 only in clade B strains, not clade C strains	1231:1296	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	6	39	from	N295	1248:1251	arg1	presence					1219:1226	the presence	1215:1226	the presence of glycosylation at N295 only in clade B strains, not clade C strains	1215:1296	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	1	40	theme	HIV-1	286:290	arg1	gp120					292:296	HIV-1 gp120	286:296	HIV-1 gp120	286:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	1	41	gly	glycosylation	263:275	arg2	sites					277:281	high-mannose N-linked glycosylation sites	241:281	high-mannose N-linked glycosylation sites on HIV-1 gp120	241:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	2	42	theme	key	301:303	arg1	glycosylation					332:344	glycosylation	332:344	glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	332:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	2	42	theme	key	301:303	arg1	factor					305:310	A key factor	299:310	A key factor for Grft potency	299:327	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	0	43	theme	Anti-HIV-1	20:29	arg1	Potency					31:37	Anti-HIV-1 Potency	20:37	Anti-HIV-1 Potency	20:37	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	5	44	theme	glycans	1055:1061	arg1	importance					1030:1039	the importance	1026:1039	the importance of individual glycans	1026:1061	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	5	44	theme	glycans	1055:1061	arg1	related					1070:1076	related	1070:1076	related	1070:1076	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	1	45	from	sites	277:281	arg1	gp120					292:296	HIV-1 gp120	286:296	HIV-1 gp120	286:296	Griffithsin (Grft) is an antiviral lectin that has been shown to potently inhibit HIV-1 by binding high-mannose N-linked glycosylation sites on HIV-1 gp120.					
31611356	9	46	dep	important	1639:1647	arg1	therapeutic					1663:1673	therapeutic	1663:1673	therapeutic	1663:1673	This indicates that Grft could be an important combinational therapeutic.					
31611356	2	47	theme	entire	423:428	arg1	class					430:434	an entire class	420:434	an entire class of broadly neutralizing antibodies (bNAbs)	420:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	5	48	dep	N295	900:903	arg1	the					885:887	the	885:887	the	885:887	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	5	48	dep	N295	900:903	arg1	absence					889:895	absence	889:895	absence	889:895	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	6	49	theme	clade	1261:1265	arg1	strains					1269:1275	clade B strains	1261:1275	clade B strains	1261:1275	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	6	50	theme	binding	1185:1191	arg1	site					1193:1196	the CD4 binding site	1177:1196	the CD4 binding site	1177:1196	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	4	51	theme	moderate	833:840	arg1	effects					842:848	moderate effects	833:848	moderate effects on Grft potency	833:864	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	7	52	theme	bNAbs	1363:1367	arg1	activity					1347:1354	the activity	1343:1354	the activity of the bNAbs PGT121 and PGT126	1343:1385	We further demonstrate that Grft can rescue the activity of the bNAbs PGT121 and PGT126 in the event of a loss or a shift of glycosylation at N332, where the bNAbs suffer a drastic loss of potency.					
31611356	3	53	theme	glycans	536:542	arg1	importance					516:525	the importance	512:525	the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120	512:689	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	8	54	theme	same	1519:1522	arg1	region					1524:1529	the same region	1515:1529	the same region	1515:1529	Despite targeting the same region, Grft in combination with PGT121 and PGT126 produced additive effects.					
31611356	6	55	theme	glycosylation	1231:1243	arg1	presence					1219:1226	the presence	1215:1226	the presence of glycosylation at N295 only in clade B strains, not clade C strains	1215:1296	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	6	56	theme	CD4	1181:1183	arg1	site					1193:1196	the CD4 binding site	1177:1196	the CD4 binding site	1177:1196	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	4	57	theme	activity	734:741	arg1	loss					721:724	a significant loss	707:724	a significant loss of Grft activity by the removal of glycosylation at N295	707:781	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	0	58	theme	gp120	55:59	arg1	Glycosylation					61:73	gp120 Glycosylation	55:73	gp120 Glycosylation	55:73	Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.					
31611356	4	59	from	N448	810:813	arg1	absence					791:797	glycan absence	784:797	glycan absence at N332 or N448	784:813	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	5	60	theme	at	984:985	arg1	N448					987:990	the glycan at N448	973:990	the glycan at N448 shifting to N446	973:1007	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	2	61	gly	glycosylation	332:344	arg1	N295					349:352	N295	349:352	N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	349:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	2	61	gly	glycosylation	332:344	arg2	N295					349:352	N295	349:352	N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	349:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	3	62	theme	other	530:534	arg1	glycans					536:542	other glycans	530:542	other glycans	530:542	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	3	63	from	work	504:507	arg1	importance					516:525	the importance	512:525	the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120	512:689	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	6	64	from	strains	1290:1296	arg1	strains					1269:1275	clade B strains	1261:1275	clade B strains	1261:1275	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	5	65	from	effect	1087:1092	arg1	density					1111:1117	glycosylation density	1097:1117	glycosylation density	1097:1117	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	3	66	theme	conformational	607:620	arg1	change					622:627	the conformational change	603:627	the conformational change of gp120	603:636	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	6	67	gly	glycosylation	1231:1243	arg2	N295					1248:1251	N295	1248:1251	N295	1248:1251	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	6	67	gly	glycosylation	1231:1243	arg1	N295					1248:1251	N295	1248:1251	N295	1248:1251	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	5	68	theme	Grft	906:909	arg1	effectiveness					911:923	Grft effectiveness	906:923	Grft effectiveness	906:923	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	6	69	theme	gp120	1161:1165	arg1	structure					1148:1156	the structure	1144:1156	the structure of gp120	1144:1165	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	4	70	theme	significant	709:719	arg1	loss					721:724	a significant loss	707:724	a significant loss of Grft activity by the removal of glycosylation at N295	707:781	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	2	71	theme	Grft	316:319	arg1	potency					321:327	Grft potency	316:327	Grft potency	316:327	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	4	72	from	N332	802:805	arg1	absence					791:797	glycan absence	784:797	glycan absence at N332 or N448	784:813	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	3	73	theme	Grft	547:550	arg1	potency					552:558	Grft potency	547:558	Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120	547:689	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	2	74	theme	gp120	357:361	arg1	N295					349:352	N295	349:352	N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs)	349:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	5	75	theme	glycosylation	1097:1109	arg1	density					1111:1117	glycosylation density	1097:1117	glycosylation density	1097:1117	Interestingly, in the absence of N295, Grft effectiveness could be improved by a mutation that results in the glycan at N448 shifting to N446, indicating that the importance of individual glycans may be related to their effect on glycosylation density.					
31611356	2	76	theme	antibodies	460:469	arg1	class					430:434	an entire class	420:434	an entire class of broadly neutralizing antibodies (bNAbs)	420:477	A key factor for Grft potency is glycosylation at N295 of gp120, which is directly adjacent to N332, a target glycan for an entire class of broadly neutralizing antibodies (bNAbs).					
31611356	3	77	theme	gp120	632:636	arg1	change					622:627	the conformational change	603:627	the conformational change of gp120	603:636	Here, we unify previous work on the importance of other glycans to Grft potency against HIV-1 and Grft's role in mediating the conformational change of gp120 by mutating nearly every glycosylation site in gp120.					
31611356	6	78	theme	B	1267:1267	arg1	strains					1269:1275	clade B strains	1261:1275	clade B strains	1261:1275	Grft's ability to alter the structure of gp120, exposing the CD4 binding site, correlated with the presence of glycosylation at N295 only in clade B strains, not clade C strains.					
31611356	4	79	attach	removal	750:756	arg3	N295					778:781	N295	778:781	N295	778:781	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
31611356	4	79	attach	removal	750:756	arg2	glycosylation					761:773	glycosylation	761:773	glycosylation	761:773	In addition to a significant loss of Grft activity by the removal of glycosylation at N295, glycan absence at N332 or N448 was found to have moderate effects on Grft potency.					
30592755	12	0	theme	cancers	1877:1883	arg1	some					1759:1762	some	1759:1762	some	1759:1762	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	12	0	theme	cancers	1877:1883	arg1	glycoforms					1771:1780	the glycoforms	1767:1780	the glycoforms greatly elevated in the Ethiopian subjects	1767:1823	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	12	0	theme	cancers	1877:1883	arg1	biomarkers					1855:1864	serum biomarkers	1849:1864	serum biomarkers of various cancers	1849:1883	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	3	1	from	serum	530:534	arg1	METHODS					516:522	METHODS	516:522	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients	516:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	13	2	dep	increased	1922:1930	arg1	compared					1957:1964	compared	1957:1964	compared to the other ethnicities	1957:1989	Sialic acid level was significantly increased primarily in Ethiopians, compared to the other ethnicities.					
30592755	14	3	theme	most	2109:2112	arg1	glycoforms					2114:2123	most glycoforms	2109:2123	most glycoforms	2109:2123	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	3	4	theme	ethnicity	572:580	arg1	subjects					552:559	54 healthy subjects	541:559	54 healthy subjects of various ethnicity	541:580	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	3	4	theme	ethnicity	572:580	arg1	patients					629:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	5	5	dep	N-glycan	868:875	arg1	levels					892:897	levels	892:897	levels	892:897	Data representing serum N-glycan or sialic acid levels were compared among the ethnic groups using SPSS software.					
30592755	12	6	theme	serum	1849:1853	arg1	some					1759:1762	some	1759:1762	some	1759:1762	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	12	6	theme	serum	1849:1853	arg1	glycoforms					1771:1780	the glycoforms	1767:1780	the glycoforms greatly elevated in the Ethiopian subjects	1767:1823	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	12	6	theme	serum	1849:1853	arg1	biomarkers					1855:1864	serum biomarkers	1849:1864	serum biomarkers of various cancers	1849:1883	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	10	7	theme	Japanese	1609:1616	arg1	patients					1622:1629	Japanese HCC patients	1609:1629	Japanese HCC patients	1609:1629	Glycan abundance trend in Ethiopians was generally close to that of Japanese HCC patients.					
30592755	11	8	from	disparities	1683:1693	arg1	structures					1733:1742	the branched and sialylated structures	1705:1742	the branched and sialylated structures	1705:1742	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	12	9	theme	Ethiopian	1806:1814	arg1	subjects					1816:1823	the Ethiopian subjects	1802:1823	the Ethiopian subjects	1802:1823	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	7	10	theme	ethnic	1241:1246	arg1	glycans					1258:1264	5 glycans	1256:1264	5 glycans	1256:1264	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	7	10	theme	ethnic	1241:1246	arg1	groups					1248:1253	all the other ethnic groups	1227:1253	all the other ethnic groups (5 glycans)	1227:1265	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	4	11	theme	glycoblotting-assisted	694:715	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	3	12	theme	hepatocellular	598:611	arg1	HCC					624:626	HCC	624:626	HCC	624:626	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	3	12	theme	hepatocellular	598:611	arg1	carcinoma					613:621	hepatocellular carcinoma	598:621	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	6	13	gly	glycoproteins	1014:1026	arg1	glycoproteins					1014:1026	whole serum glycoproteins	1002:1026	whole serum glycoproteins	1002:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	11	14	gly	sialylated	1722:1731	arg1	structures					1733:1742	the branched and sialylated structures	1705:1742	the branched and sialylated structures	1705:1742	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	16	15	theme	large-scale	2278:2288	arg1	study					2290:2294	Further large-scale study	2270:2294	Further large-scale study employing various ethnic compositions	2270:2332	Further large-scale study employing various ethnic compositions is needed to verify the current result.					
30592755	8	16	theme	Highest	1268:1274	arg1	abundance					1276:1284	Highest abundance	1268:1284	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1268:1371	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	8	16	theme	Highest	1268:1274	arg1	<					1289:1289	p < 0.001	1287:1295	p < 0.001	1287:1295	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	5	17	theme	ethnic	923:928	arg1	groups					930:935	the ethnic groups	919:935	the ethnic groups using SPSS software	919:955	Data representing serum N-glycan or sialic acid levels were compared among the ethnic groups using SPSS software.					
30592755	4	18	theme	N-glycome	767:775	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	4	18	theme	N-glycome	767:775	arg1	quantification					805:818	fluorescence HPLC-based quantification	781:818	fluorescence HPLC-based quantification of sialic acid species	781:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	16	19	theme	ethnic	2314:2319	arg1	compositions					2321:2332	various ethnic compositions	2306:2332	various ethnic compositions	2306:2332	Further large-scale study employing various ethnic compositions is needed to verify the current result.					
30592755	6	20	theme	whole	1002:1006	arg1	glycoproteins					1014:1026	whole serum glycoproteins	1002:1026	whole serum glycoproteins	1002:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	8	21	theme	N-glycans	1363:1371	arg1	abundance					1276:1284	Highest abundance	1268:1284	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1268:1371	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	8	21	theme	N-glycans	1363:1371	arg1	<					1289:1289	p < 0.001	1287:1295	p < 0.001	1287:1295	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	6	22	located	detected	1093:1100	arg1	which					1068:1072	which	1068:1072	which	1068:1072	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	6	22	located	detected	1093:1100	arg1	ethnicities					1109:1119	all ethnicities	1105:1119	all ethnicities	1105:1119	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	6	22	located	detected	1093:1100	arg2	glycoforms					1077:1086	33 glycoforms	1074:1086	33 glycoforms	1074:1086	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	14	23	from	differences	2038:2048	arg1	N-glycome					2073:2081	healthy human serum N-glycome	2053:2081	healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2053:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	1	24	theme	Most	145:148	arg1	studies					160:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	4	25	theme	fluorescence	781:792	arg1	quantification					805:818	fluorescence HPLC-based quantification	781:818	fluorescence HPLC-based quantification of sialic acid species	781:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	4	26	theme	quantitative	736:747	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	7	27	theme	other	1235:1239	arg1	glycans					1258:1264	5 glycans	1256:1264	5 glycans	1256:1264	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	7	27	theme	other	1235:1239	arg1	groups					1248:1253	all the other ethnic groups	1227:1253	all the other ethnic groups (5 glycans)	1227:1265	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	13	28	theme	other	1973:1977	arg1	ethnicities					1979:1989	the other ethnicities	1969:1989	the other ethnicities	1969:1989	Sialic acid level was significantly increased primarily in Ethiopians, compared to the other ethnicities.					
30592755	0	29	theme	variation	71:79	arg1	influence					51:59	the influence	47:59	the influence of ethnic variation on the identified cancer-relevant glycan biomarkers	47:131	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	4	30	theme	species	835:841	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	4	30	theme	species	835:841	arg1	quantification					805:818	fluorescence HPLC-based quantification	781:818	fluorescence HPLC-based quantification of sialic acid species	781:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	8	31	dep	mannose	1306:1312	arg1	hyperbranched/hypersialylated					1333:1361	hyperbranched/hypersialylated	1333:1361	hyperbranched/hypersialylated	1333:1361	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	8	31	dep	mannose	1306:1312	arg1	core-fucosylated					1315:1330	core-fucosylated	1315:1330	core-fucosylated	1315:1330	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	14	32	dep	CONCLUSION	1992:2001	arg1	revealed					2013:2020	revealed	2013:2020	revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2013:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	0	33	theme	cancer-relevant	99:113	arg1	biomarkers					122:131	the identified cancer-relevant glycan biomarkers	84:131	the identified cancer-relevant glycan biomarkers	84:131	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	14	34	from	abundance	2096:2104	arg1	Ethiopian					2132:2140	Ethiopian	2132:2140	Ethiopian	2132:2140	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	14	35	theme	glycoforms	2114:2123	arg1	abundance					2096:2104	highest abundance	2088:2104	highest abundance of most glycoforms in the Ethiopian ethnicity	2088:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	2	36	theme	serum	414:418	arg1	variation					429:437	the inter-ethnic serum N-glycan variation	397:437	the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers	397:513	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	0	37	theme	Healthy	0:6	arg1	profiling					29:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	1	38	theme	ethnic	288:293	arg1	variation					295:303	ethnic variation	288:303	ethnic variation	288:303	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	4	39	theme	sialic	823:828	arg1	species					835:841	sialic acid species	823:841	sialic acid species	823:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	6	40	attach	released	988:995	arg1	glycoproteins					1014:1026	whole serum glycoproteins	1002:1026	whole serum glycoproteins	1002:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	6	40	attach	released	988:995	arg2	N-glycans					978:986	51 N-glycans	975:986	51 N-glycans released from whole serum glycoproteins	975:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	0	41	theme	serum	14:18	arg1	profiling					29:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	14	42	theme	highest	2088:2094	arg1	abundance					2096:2104	highest abundance	2088:2104	highest abundance of most glycoforms in the Ethiopian ethnicity	2088:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	2	43	theme	healthy	496:502	arg1	volunteers					504:513	healthy volunteers	496:513	healthy volunteers	496:513	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	10	44	theme	Glycan	1541:1546	arg1	trend					1558:1562	Glycan abundance trend	1541:1562	Glycan abundance trend in Ethiopians	1541:1576	Glycan abundance trend in Ethiopians was generally close to that of Japanese HCC patients.					
30592755	2	45	dep	US	445:446	arg1	Indian					474:479	Indian	474:479	Indian	474:479	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	45	dep	US	445:446	arg1	Ethiopian					486:494	Ethiopian	486:494	Ethiopian	486:494	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	45	dep	US	445:446	arg1	Japanese					464:471	Japanese	464:471	Japanese	464:471	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	45	dep	US	445:446	arg1	control					455:461	origin control	448:461	origin control	448:461	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	5	46	theme	serum	862:866	arg1	N-glycan					868:875	serum N-glycan	862:875	serum N-glycan	862:875	Data representing serum N-glycan or sialic acid levels were compared among the ethnic groups using SPSS software.					
30592755	1	47	theme	identified	312:321	arg1	glyco-biomarker					323:337	the identified glyco-biomarker	308:337	the identified glyco-biomarker	308:337	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	14	48	theme	human	2061:2065	arg1	N-glycome					2073:2081	healthy human serum N-glycome	2053:2081	healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2053:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	12	49	theme	various	1869:1875	arg1	cancers					1877:1883	various cancers	1869:1883	various cancers	1869:1883	Surprisingly, some of the glycoforms greatly elevated in the Ethiopian subjects have been identified as serum biomarkers of various cancers.					
30592755	13	50	theme	Sialic	1886:1891	arg1	level					1898:1902	Sialic acid level	1886:1902	Sialic acid level	1886:1902	Sialic acid level was significantly increased primarily in Ethiopians, compared to the other ethnicities.					
30592755	14	51	theme	ethnic-specific	2022:2036	arg1	differences					2038:2048	ethnic-specific differences	2022:2048	ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2022:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	11	52	theme	sialylated	1722:1731	arg1	structures					1733:1742	the branched and sialylated structures	1705:1742	the branched and sialylated structures	1705:1742	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	6	53	theme	N-glycans	978:986	arg1	Total					966:970	Total	966:970	RESULTS Total of 51 N-glycans released from whole serum glycoproteins	958:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	16	54	theme	current	2358:2364	arg1	result					2366:2371	the current result	2354:2371	the current result	2354:2371	Further large-scale study employing various ethnic compositions is needed to verify the current result.					
30592755	11	55	theme	branched	1709:1716	arg1	structures					1733:1742	the branched and sialylated structures	1705:1742	the branched and sialylated structures	1705:1742	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	3	56	theme	various	564:570	arg1	ethnicity					572:580	various ethnicity	564:580	various ethnicity	564:580	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	10	57	theme	HCC	1618:1620	arg1	patients					1622:1629	Japanese HCC patients	1609:1629	Japanese HCC patients	1609:1629	Glycan abundance trend in Ethiopians was generally close to that of Japanese HCC patients.					
30592755	5	58	theme	SPSS	943:946	arg1	software					948:955	SPSS software	943:955	SPSS software	943:955	Data representing serum N-glycan or sialic acid levels were compared among the ethnic groups using SPSS software.					
30592755	8	59	theme	p	1287:1287	arg1	abundance					1276:1284	Highest abundance	1268:1284	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1268:1371	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	8	59	theme	p	1287:1287	arg1	<					1289:1289	p < 0.001	1287:1295	p < 0.001	1287:1295	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	16	60	theme	Further	2270:2276	arg1	study					2290:2294	Further large-scale study	2270:2294	Further large-scale study employing various ethnic compositions	2270:2332	Further large-scale study employing various ethnic compositions is needed to verify the current result.					
30592755	3	61	theme	Japanese	589:596	arg1	patients					629:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	1	62	theme	disease	198:204	arg1	mechanisms					206:215	disease mechanisms	198:215	disease mechanisms	198:215	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	4	63	theme	comprehensive	680:692	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	1	64	from	influence	275:283	arg1	glyco-biomarker					323:337	the identified glyco-biomarker	308:337	the identified glyco-biomarker	308:337	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	15	65	theme	accurate	2229:2236	arg1	identification					2254:2267	accurate glyco-biomarker identification	2229:2267	accurate glyco-biomarker identification	2229:2267	The results strongly emphasized the need to consider ethnicity matching for accurate glyco-biomarker identification.					
30592755	16	66	theme	various	2306:2312	arg1	compositions					2321:2332	various ethnic compositions	2306:2332	various ethnic compositions	2306:2332	Further large-scale study employing various ethnic compositions is needed to verify the current result.					
30592755	1	67	theme	serum	231:235	arg1	markers					237:243	serum markers	231:243	serum markers for various diseases	231:264	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	8	68	theme	mannose	1306:1312	arg1	N-glycans					1363:1371	high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1301:1371	high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1301:1371	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	7	69	theme	remaining	1126:1134	arg1	N-glycans					1136:1144	The remaining N-glycans	1122:1144	The remaining N-glycans	1122:1144	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	3	70	theme	carcinoma	613:621	arg1	patients					629:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	6	71	theme	serum	1008:1012	arg1	glycoproteins					1014:1026	whole serum glycoproteins	1002:1026	whole serum glycoproteins	1002:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	4	72	theme	serum	761:765	arg1	N-glycome					767:775	serum N-glycome	761:775	serum N-glycome	761:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	1	73	theme	BACKGROUND	134:143	arg1	studies					160:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	0	74	theme	ethnic	64:69	arg1	variation					71:79	ethnic variation	64:79	ethnic variation	64:79	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	8	75	theme	high	1301:1304	arg1	N-glycans					1363:1371	high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1301:1371	high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans	1301:1371	Highest abundance (p < 0.001) of high mannose, core-fucosylated, hyperbranched/hypersialylated N-glycans was demonstrated in Ethiopians.					
30592755	1	76	theme	glycomics	150:158	arg1	studies					160:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies	134:166	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	10	77	from	trend	1558:1562	arg1	Ethiopians					1567:1576	Ethiopians	1567:1576	Ethiopians	1567:1576	Glycan abundance trend in Ethiopians was generally close to that of Japanese HCC patients.					
30592755	4	78	theme	MALDI-TOF/MS-based	717:734	arg1	analysis					749:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis	678:756	a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome	678:775	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	0	79	theme	identified	88:97	arg1	biomarkers					122:131	the identified cancer-relevant glycan biomarkers	84:131	the identified cancer-relevant glycan biomarkers	84:131	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	14	80	with	N-glycome	2073:2081	arg1	abundance					2096:2104	highest abundance	2088:2104	highest abundance of most glycoforms in the Ethiopian ethnicity	2088:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	0	81	from	influence	51:59	arg1	biomarkers					122:131	the identified cancer-relevant glycan biomarkers	84:131	the identified cancer-relevant glycan biomarkers	84:131	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	4	82	theme	acid	830:833	arg1	species					835:841	sialic acid species	823:841	sialic acid species	823:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	0	83	theme	glycan	115:120	arg1	biomarkers					122:131	the identified cancer-relevant glycan biomarkers	84:131	the identified cancer-relevant glycan biomarkers	84:131	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	2	84	theme	N-glycan	420:427	arg1	variation					429:437	the inter-ethnic serum N-glycan variation	397:437	the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers	397:513	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	5	85	theme	sialic	880:885	arg1	acid					887:890	sialic acid	880:890	sialic acid	880:890	Data representing serum N-glycan or sialic acid levels were compared among the ethnic groups using SPSS software.					
30592755	2	86	dep	Indian	474:479	arg1	volunteers					504:513	healthy volunteers	496:513	healthy volunteers	496:513	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	3	87	dep	serum	530:534	arg1	subjects					552:559	54 healthy subjects	541:559	54 healthy subjects of various ethnicity	541:580	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	3	87	dep	serum	530:534	arg1	patients					629:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	11 Japanese hepatocellular carcinoma (HCC) patients	586:636	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	4	88	theme	HPLC-based	794:803	arg1	quantification					805:818	fluorescence HPLC-based quantification	781:818	fluorescence HPLC-based quantification of sialic acid species	781:841	We employed a comprehensive glycoblotting-assisted MALDI-TOF/MS-based quantitative analysis of serum N-glycome and fluorescence HPLC-based quantification of sialic acid species.					
30592755	2	89	theme	inter-ethnic	401:412	arg1	variation					429:437	the inter-ethnic serum N-glycan variation	397:437	the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers	397:513	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	0	90	theme	N-glycan	20:27	arg1	profiling					29:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30592755	1	91	theme	variation	295:303	arg1	influence					275:283	the influence	271:283	the influence of ethnic variation on the identified glyco-biomarker	271:337	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	10	92	theme	abundance	1548:1556	arg1	trend					1558:1562	Glycan abundance trend	1541:1562	Glycan abundance trend in Ethiopians	1541:1576	Glycan abundance trend in Ethiopians was generally close to that of Japanese HCC patients.					
30592755	7	93	located	detected	1151:1158	arg2	N-glycans					1136:1144	The remaining N-glycans	1122:1144	The remaining N-glycans	1122:1144	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	7	93	located	detected	1151:1158	arg1	Ethiopians					1197:1206	the Ethiopians	1193:1206	the Ethiopians (13 glycans)	1193:1219	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	7	93	located	detected	1151:1158	arg1	glycans					1212:1218	13 glycans	1209:1218	13 glycans	1209:1218	The remaining N-glycans were detected weakly but exclusively either in the Ethiopians (13 glycans) or in all the other ethnic groups (5 glycans).					
30592755	2	94	theme	origin	448:453	arg1	Indian					474:479	Indian	474:479	Indian	474:479	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	94	theme	origin	448:453	arg1	Ethiopian					486:494	Ethiopian	486:494	Ethiopian	486:494	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	94	theme	origin	448:453	arg1	Japanese					464:471	Japanese	464:471	Japanese	464:471	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	2	94	theme	origin	448:453	arg1	control					455:461	origin control	448:461	origin control	448:461	This study aimed to investigate the inter-ethnic serum N-glycan variation among US origin control, Japanese, Indian, and Ethiopian healthy volunteers.					
30592755	15	95	theme	glyco-biomarker	2238:2252	arg1	identification					2254:2267	accurate glyco-biomarker identification	2229:2267	accurate glyco-biomarker identification	2229:2267	The results strongly emphasized the need to consider ethnicity matching for accurate glyco-biomarker identification.					
30592755	14	96	theme	serum	2067:2071	arg1	N-glycome					2073:2081	healthy human serum N-glycome	2053:2081	healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2053:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	11	97	theme	Glycotyping	1632:1642	arg1	analysis					1644:1651	Glycotyping analysis	1632:1651	Glycotyping analysis	1632:1651	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	3	98	theme	Human	524:528	arg1	serum					530:534	Human serum	524:534	Human serum	524:534	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	14	99	theme	healthy	2053:2059	arg1	N-glycome					2073:2081	healthy human serum N-glycome	2053:2081	healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity	2053:2150	CONCLUSION The study revealed ethnic-specific differences in healthy human serum N-glycome with highest abundance of most glycoforms in the Ethiopian ethnicity.					
30592755	6	100	dep	RESULTS	958:964	arg1	Total					966:970	Total	966:970	RESULTS Total of 51 N-glycans released from whole serum glycoproteins	958:1026	RESULTS Total of 51 N-glycans released from whole serum glycoproteins could be reproducibly quantified within which 33 glycoforms were detected in all ethnicities.					
30592755	3	101	theme	healthy	544:550	arg1	subjects					552:559	54 healthy subjects	541:559	54 healthy subjects of various ethnicity	541:580	METHODS Human serum from 54 healthy subjects of various ethnicity and 11 Japanese hepatocellular carcinoma (HCC) patients were included in the study.					
30592755	1	102	theme	various	249:255	arg1	diseases					257:264	various diseases	249:264	various diseases	249:264	BACKGROUND Most glycomics studies have focused on understanding disease mechanisms and proposing serum markers for various diseases, yet the influence of ethnic variation on the identified glyco-biomarker remains poorly addressed.					
30592755	13	103	theme	acid	1893:1896	arg1	level					1898:1902	Sialic acid level	1886:1902	Sialic acid level	1886:1902	Sialic acid level was significantly increased primarily in Ethiopians, compared to the other ethnicities.					
30592755	11	104	theme	ethnic-based	1670:1681	arg1	disparities					1683:1693	ethnic-based disparities	1670:1693	ethnic-based disparities mainly in the branched and sialylated structures	1670:1742	Glycotyping analysis further revealed ethnic-based disparities mainly in the branched and sialylated structures.					
30592755	0	105	theme	human	8:12	arg1	profiling					29:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling	0:37	Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.					
30898876	5	0	theme	receptor-related	1214:1229	arg1	protein					1231:1237	protein 1	1231:1239	protein 1	1231:1239	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	8	1	theme	mammalian	1765:1773	arg1	glycoproteins					1775:1787	mammalian glycoproteins	1765:1787	mammalian glycoproteins carrying type-I LDN	1765:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	1	2	theme	type-II	268:274	arg1	GalNAcβ1-4GlcNAc					292:307	GalNAcβ1-4GlcNAc	292:307	GalNAcβ1-4GlcNAc	292:307	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	2	theme	type-II	268:274	arg1	structures					280:289	type-II LDN structures	268:289	type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones	268:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	3	3	theme	HeLa	662:665	arg1	cells					667:671	HeLa cells	662:671	HeLa cells	662:671	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	2	4	theme	carrier	597:603	arg1	s					613:613	s	613:613	s	613:613	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	2	4	theme	carrier	597:603	arg1	protein					605:611	its carrier protein	593:611	its carrier protein(s)	593:614	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	1	5	theme	LDN	276:278	arg1	GalNAcβ1-4GlcNAc					292:307	GalNAcβ1-4GlcNAc	292:307	GalNAcβ1-4GlcNAc	292:307	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	5	theme	LDN	276:278	arg1	structures					280:289	type-II LDN structures	268:289	type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones	268:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	5	6	from	presence	1178:1185	arg1	protein					1231:1237	protein 1	1231:1239	protein 1	1231:1239	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	5	6	from	presence	1178:1185	arg1	nicastrin					1245:1253	nicastrin	1245:1253	nicastrin	1245:1253	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	1	7	theme	mammalian	195:203	arg1	cells					205:209	mammalian cells	195:209	mammalian cells	195:209	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	2	8	theme	LDN	549:551	arg1	function					530:537	the function	526:537	the function of type-I LDN in mammalian cells	526:570	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	2	8	theme	LDN	549:551	arg1	unclear					581:587	unclear	581:587	unclear	581:587	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	4	9	theme	B3GALNT2-generated	1090:1107	arg1	LDN					1116:1118	B3GALNT2-generated type-I LDN	1090:1118	B3GALNT2-generated type-I LDN in their N-glycans	1090:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	1	10	located	observed	183:190	arg2	LacdiNAc					134:141	The type-I LacdiNAc	123:141	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc)	123:165	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	10	located	observed	183:190	arg1	O-glycan					225:232	the O-glycan	221:232	the O-glycan of α-dystroglycan	221:250	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	10	located	observed	183:190	arg1	cells					205:209	mammalian cells	195:209	mammalian cells	195:209	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	10	located	observed	183:190	arg2	LDN					144:146	LDN	144:146	LDN; GalNAcβ1-3GlcNAc	144:164	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	11	gly	glycoproteins	365:377	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	11	gly	glycoproteins	365:377	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	4	12	from	LDN	1116:1118	arg1	N-glycans					1129:1137	their N-glycans	1123:1137	their N-glycans	1123:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	7	13	theme	type-II	1698:1704	arg1	LDNs					1706:1709	type-I or type-II LDNs	1688:1709	type-I or type-II LDNs	1688:1709	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	8	14	gly	glycoproteins	1775:1787	arg1	glycoproteins					1775:1787	mammalian glycoproteins	1765:1787	mammalian glycoproteins carrying type-I LDN	1765:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	7	15	gly	glycoproteins	1558:1570	arg1	glycoproteins					1558:1570	extracellular glycoproteins	1544:1570	extracellular glycoproteins	1544:1570	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	2	16	theme	a	434:434	arg1	β1,3-N-acetylgalactosaminyltransferase					436:473	a β1,3-N-acetylgalactosaminyltransferase 2	434:475	a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase)	434:507	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	2	16	theme	a	434:434	arg1	B3GALNT2					478:485	B3GALNT2	478:485	B3GALNT2; type-I LDN synthase	478:506	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	7	17	theme	type-I	1688:1693	arg1	LDNs					1706:1709	type-I or type-II LDNs	1688:1709	type-I or type-II LDNs	1688:1709	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	4	18	theme	isotope-coded	872:884	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	2	19	theme	mammalian	556:564	arg1	cells					566:570	mammalian cells	556:570	mammalian cells	556:570	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	3	20	theme	Golgi-resident	708:721	arg1	glycosyltransferase					723:741	Golgi-resident glycosyltransferase	708:741	Golgi-resident glycosyltransferase	708:741	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	8	21	theme	type-I	1839:1844	arg1	LDNs					1858:1861	type-I and type-II LDNs	1839:1861	type-I and type-II LDNs	1839:1861	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	3	22	theme	floribunda	837:846	arg1	WFA					860:862	WFA	860:862	WFA	860:862	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	3	22	theme	floribunda	837:846	arg1	agglutinin					848:857	Wisteria floribunda agglutinin	828:857	Wisteria floribunda agglutinin (WFA)	828:863	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	4	23	theme	site-specific	900:912	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	3	24	theme	B3GALNT2-synthesized	770:789	arg1	structures					802:811	B3GALNT2-synthesized type-I LDN structures	770:811	B3GALNT2-synthesized type-I LDN structures	770:811	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	8	25	theme	type-II	1850:1856	arg1	LDNs					1858:1861	type-I and type-II LDNs	1839:1861	type-I and type-II LDNs	1839:1861	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	4	26	theme	hamster	960:966	arg1	cells					968:972	Lec8 Chinese hamster cells	947:972	Lec8 Chinese hamster cells lacking galactosylation	947:996	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	8	27	attach	presence	1753:1760	arg1	N-glycans					1812:1820	N-glycans	1812:1820	N-glycans	1812:1820	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	8	27	attach	presence	1753:1760	arg2	glycoproteins					1775:1787	mammalian glycoproteins	1765:1787	mammalian glycoproteins carrying type-I LDN	1765:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	5	28	theme	LDN	1312:1314	arg1	occurrence					1291:1300	the occurrence	1287:1300	the occurrence of type-I LDN	1287:1314	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	6	29	theme	identified	1389:1398	arg1	glycoproteins					1400:1412	the identified glycoproteins	1385:1412	the identified glycoproteins	1385:1412	Our analysis also uncovered that most of the identified glycoproteins localize to intracellular organelles, particularly to the endoplasmic reticulum.					
30898876	1	30	dep	structures	280:289	arg1	contrast					256:263	contrast	256:263	contrast	256:263	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	31	dep	LDN	144:146	arg1	GalNAcβ1-3GlcNAc					149:164	GalNAcβ1-3GlcNAc	149:164	LDN; GalNAcβ1-3GlcNAc	144:164	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	3	32	theme	LDN	798:800	arg1	structures					802:811	B3GALNT2-synthesized type-I LDN structures	770:811	B3GALNT2-synthesized type-I LDN structures	770:811	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	4	33	theme	Lec8	947:950	arg1	cells					968:972	Lec8 Chinese hamster cells	947:972	Lec8 Chinese hamster cells lacking galactosylation	947:996	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	1	34	theme	mammalian	355:363	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	34	theme	mammalian	355:363	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	4	35	contain	carry	1084:1088	arg1	glycoproteins					1065:1077	the glycoproteins	1061:1077	the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans	1061:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	4	35	contain	carry	1084:1088	arg2	LDN					1116:1118	B3GALNT2-generated type-I LDN	1090:1118	B3GALNT2-generated type-I LDN in their N-glycans	1090:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	7	36	contain	carry	1682:1686	arg1	proteins					1668:1675	proteins	1668:1675	proteins that carry type-I or type-II LDNs	1668:1709	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	7	36	contain	carry	1682:1686	arg2	LDNs					1706:1709	type-I or type-II LDNs	1688:1709	type-I or type-II LDNs	1688:1709	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	8	37	from	presence	1753:1760	arg1	N-glycans					1812:1820	N-glycans	1812:1820	N-glycans	1812:1820	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	1	38	from	glycoproteins	365:377	arg1	present					339:345	present	339:345	present	339:345	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	5	39	theme	LDN	1174:1176	arg1	presence					1178:1185	LDN presence	1174:1185	LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin	1174:1253	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	4	40	theme	B3GALNT2	1032:1039	arg1	gene					1041:1044	the B3GALNT2 gene	1028:1044	the B3GALNT2 gene	1028:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	0	41	theme	mammalian	18:26	arg1	glycoproteins					28:40	mammalian glycoproteins	18:40	mammalian glycoproteins	18:40	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	1	42	theme	type-I	127:132	arg1	LDN					144:146	LDN	144:146	LDN; GalNAcβ1-3GlcNAc	144:164	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	42	theme	type-I	127:132	arg1	LacdiNAc					134:141	The type-I LacdiNAc	123:141	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc)	123:165	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	2	43	theme	type-I	488:493	arg1	synthase					499:506	type-I LDN synthase	488:506	B3GALNT2; type-I LDN synthase	478:506	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	5	44	theme	mammalian	1327:1335	arg1	cells					1337:1341	mammalian cells	1327:1341	mammalian cells	1327:1341	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	6	45	theme	intracellular	1426:1438	arg1	organelles					1440:1449	intracellular organelles	1426:1449	intracellular organelles	1426:1449	Our analysis also uncovered that most of the identified glycoproteins localize to intracellular organelles, particularly to the endoplasmic reticulum.					
30898876	1	46	from	present	339:345	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	46	from	present	339:345	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	4	47	theme	cells	1005:1009	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	0	48	theme	LacdiNAc	54:61	arg1	structures					63:72	type-I LacdiNAc structures	47:72	type-I LacdiNAc structures	47:72	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	7	49	theme	extracellular	1544:1556	arg1	glycoproteins					1558:1570	extracellular glycoproteins	1544:1570	extracellular glycoproteins	1544:1570	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	0	50	gly	glycoproteins	28:40	arg1	glycoproteins					28:40	mammalian glycoproteins	18:40	mammalian glycoproteins	18:40	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	0	51	with	Identification	0:13	arg1	structures					63:72	type-I LacdiNAc structures	47:72	type-I LacdiNAc structures	47:72	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	1	52	attach	present	339:345	arg2	O-glycans					320:328	O-glycans	320:328	O-glycans	320:328	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	52	attach	present	339:345	arg2	N-					313:314	N-	313:314	N-	313:314	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	52	attach	present	339:345	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	52	attach	present	339:345	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	6	53	gly	glycoproteins	1400:1412	arg1	glycoproteins					1400:1412	the identified glycoproteins	1385:1412	the identified glycoproteins	1385:1412	Our analysis also uncovered that most of the identified glycoproteins localize to intracellular organelles, particularly to the endoplasmic reticulum.					
30898876	2	54	from	function	530:537	arg1	cells					566:570	mammalian cells	556:570	mammalian cells	556:570	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	1	55	theme	α-dystroglycan	237:250	arg1	O-glycan					225:232	the O-glycan	221:232	the O-glycan of α-dystroglycan	221:250	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	7	56	gly	glycoproteins	1625:1637	arg1	glycoproteins					1625:1637	intracellular glycoproteins	1611:1637	intracellular glycoproteins	1611:1637	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	6	57	theme	endoplasmic	1472:1482	arg1	reticulum					1484:1492	the endoplasmic reticulum	1468:1492	the endoplasmic reticulum	1468:1492	Our analysis also uncovered that most of the identified glycoproteins localize to intracellular organelles, particularly to the endoplasmic reticulum.					
30898876	4	58	theme	cells	968:972	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	8	59	theme	glycoproteins	1775:1787	arg1	presence					1753:1760	the presence	1749:1760	the presence of mammalian glycoproteins carrying type-I LDN on N-glycans	1749:1820	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	1	60	from	structures	280:289	arg1	O-glycans					320:328	O-glycans	320:328	O-glycans	320:328	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	60	from	structures	280:289	arg1	N-					313:314	N-	313:314	N-	313:314	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	4	61	theme	type-I	1109:1114	arg1	LDN					1116:1118	B3GALNT2-generated type-I LDN	1090:1118	B3GALNT2-generated type-I LDN in their N-glycans	1090:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	8	62	theme	type-I	1798:1803	arg1	LDN					1805:1807	type-I LDN	1798:1807	type-I LDN	1798:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	4	63	theme	tagging	914:920	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	4	64	gly	glycoproteins	1065:1077	arg1	glycoproteins					1065:1077	the glycoproteins	1061:1077	the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans	1061:1137	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	4	65	theme	IGOT	923:926	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	1	66	theme	pituitary	388:396	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	3	67	theme	glycosyltransferase	723:741	arg1	inhibition					694:703	inhibition	694:703	inhibition of Golgi-resident glycosyltransferase	694:741	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	1	68	theme	hypothalamic	402:413	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	69	located	present	339:345	arg2	O-glycans					320:328	O-glycans	320:328	O-glycans	320:328	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	69	located	present	339:345	arg2	N-					313:314	N-	313:314	N-	313:314	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	69	located	present	339:345	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	69	located	present	339:345	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	4	70	theme	glycosylation	886:898	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	5	71	theme	type-I	1305:1310	arg1	LDN					1312:1314	type-I LDN	1305:1314	type-I LDN	1305:1314	Our results further revealed that LDN presence on low-density lipoprotein receptor-related protein 1 and nicastrin depends on B3GALNT2, indicating the occurrence of type-I LDN in vivo in mammalian cells.					
30898876	3	72	theme	type-I	791:796	arg1	structures					802:811	B3GALNT2-synthesized type-I LDN structures	770:811	B3GALNT2-synthesized type-I LDN structures	770:811	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	3	73	theme	Wisteria	828:835	arg1	WFA					860:862	WFA	860:862	WFA	860:862	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	3	73	theme	Wisteria	828:835	arg1	agglutinin					848:857	Wisteria floribunda agglutinin	828:857	Wisteria floribunda agglutinin (WFA)	828:863	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	4	74	theme	Chinese	952:958	arg1	cells					968:972	Lec8 Chinese hamster cells	947:972	Lec8 Chinese hamster cells lacking galactosylation	947:996	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	0	75	theme	glycosyltransferase	93:111	arg1	B3GALNT2					113:120	the glycosyltransferase B3GALNT2	89:120	the glycosyltransferase B3GALNT2	89:120	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	2	76	dep	B3GALNT2	478:485	arg1	synthase					499:506	type-I LDN synthase	488:506	B3GALNT2; type-I LDN synthase	478:506	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	4	77	gly	glycosylation	886:898	arg1	cells					1005:1009	cells	1005:1009	cells transfected with the B3GALNT2 gene	1005:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	4	77	gly	glycosylation	886:898	arg1	cells					968:972	Lec8 Chinese hamster cells	947:972	Lec8 Chinese hamster cells lacking galactosylation	947:996	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	7	78	theme	intracellular	1611:1623	arg1	glycoproteins					1625:1637	intracellular glycoproteins	1611:1637	intracellular glycoproteins	1611:1637	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
30898876	2	79	theme	LDN	495:497	arg1	synthase					499:506	type-I LDN synthase	488:506	B3GALNT2; type-I LDN synthase	478:506	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	8	80	theme	different	1868:1876	arg1	roles					1878:1882	different roles	1868:1882	different roles	1868:1882	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	4	81	theme	-LC/MS	928:933	arg1	analysis					935:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis	872:942	isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene	872:1044	Using isotope-coded glycosylation site-specific tagging (IGOT)-LC/MS analysis of Lec8 Chinese hamster cells lacking galactosylation and of cells transfected with the B3GALNT2 gene, we identified the glycoproteins that carry B3GALNT2-generated type-I LDN in their N-glycans.					
30898876	3	82	theme	structures	802:811	arg1	abundance					757:765	the abundance	753:765	the abundance of B3GALNT2-synthesized type-I LDN structures	753:811	In this study, using HeLa cells, we demonstrate that inhibition of Golgi-resident glycosyltransferase increases the abundance of B3GALNT2-synthesized type-I LDN structures, recognized by Wisteria floribunda agglutinin (WFA).					
30898876	1	83	theme	many	350:353	arg1	hormones					415:422	pituitary and hypothalamic hormones	388:422	pituitary and hypothalamic hormones	388:422	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	1	83	theme	many	350:353	arg1	glycoproteins					365:377	many mammalian glycoproteins	350:377	many mammalian glycoproteins	350:377	The type-I LacdiNAc (LDN; GalNAcβ1-3GlcNAc) has rarely been observed in mammalian cells except in the O-glycan of α-dystroglycan, in contrast to type-II LDN structures (GalNAcβ1-4GlcNAc) in N- and O-glycans that are present in many mammalian glycoproteins, such as pituitary and hypothalamic hormones.					
30898876	2	84	theme	type-I	542:547	arg1	LDN					549:551	type-I LDN	542:551	type-I LDN	542:551	Although a β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2; type-I LDN synthase) has been cloned, the function of type-I LDN in mammalian cells is still unclear, as its carrier protein(s) has not been identified.					
30898876	8	85	contain	carrying	1789:1796	arg1	glycoproteins					1775:1787	mammalian glycoproteins	1765:1787	mammalian glycoproteins carrying type-I LDN	1765:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	8	85	contain	carrying	1789:1796	arg2	LDN					1805:1807	type-I LDN	1798:1807	type-I LDN	1798:1807	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	0	86	theme	glycoproteins	28:40	arg1	Identification					0:13	Identification	0:13	Identification of mammalian glycoproteins with type-I LacdiNAc structures	0:72	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	8	87	contain	have	1863:1866	arg1	LDNs					1858:1861	type-I and type-II LDNs	1839:1861	type-I and type-II LDNs	1839:1861	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	8	87	contain	have	1863:1866	arg2	roles					1878:1882	different roles	1868:1882	different roles	1868:1882	Taken together, our results indicate the presence of mammalian glycoproteins carrying type-I LDN on N-glycans and suggest that type-I and type-II LDNs have different roles in vivo.					
30898876	0	88	theme	type-I	47:52	arg1	structures					63:72	type-I LacdiNAc structures	47:72	type-I LacdiNAc structures	47:72	Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.					
30898876	7	89	from	LDN	1537:1539	arg1	glycoproteins					1558:1570	extracellular glycoproteins	1544:1570	extracellular glycoproteins	1544:1570	Whereas B4GALNT3 and B4GALNT4 synthesized LDN on extracellular glycoproteins, B3GALNT2 primarily transferred LDN to intracellular glycoproteins, thereby clearly delineating proteins that carry type-I or type-II LDNs.					
31647243	7	0	theme	RAW264.7	676:683	arg1	line					690:693	the RAW264.7 cell line	672:693	the RAW264.7 cell line	672:693	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	5	1	used	used	430:433	arg2	We					377:378	We	377:378	We	377:378	We isolated and purified 30CYGP from Chinese yam and used that material to release the N-glycans contained within.					
31647243	5	2	theme	Chinese	414:420	arg1	yam					422:424	Chinese yam	414:424	Chinese yam	414:424	We isolated and purified 30CYGP from Chinese yam and used that material to release the N-glycans contained within.					
31647243	7	3	theme	30CYGP	662:667	arg1	immunoactivities					615:630	the immunoactivities	611:630	the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line	611:693	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	3	4	theme	strong	204:209	arg1	activities					228:237	strong immunomodulatory activities	204:237	strong immunomodulatory activities	204:237	glycoprotein, 30CYGP, exhibits strong immunomodulatory activities.					
31647243	11	5	theme	Further	1027:1033	arg1	research					1035:1042	Further research	1027:1042	Further research on the role of varied glycosylation patterns in immunoactivity	1027:1105	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	9	6	contain	had	891:893	arg1	30CYGP					884:889	de-glycosylated 30CYGP	868:889	de-glycosylated 30CYGP	868:889	Furthermore, de-glycosylated 30CYGP had significantly weaker immunoactivity than 30CYGP.					
31647243	9	6	contain	had	891:893	arg2	immunoactivity					916:929	significantly weaker immunoactivity	895:929	significantly weaker immunoactivity	895:929	Furthermore, de-glycosylated 30CYGP had significantly weaker immunoactivity than 30CYGP.					
31647243	7	7	from	immunoactivities	615:630	arg1	line					690:693	the RAW264.7 cell line	672:693	the RAW264.7 cell line	672:693	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	9	8	theme	weaker	909:914	arg1	immunoactivity					916:929	significantly weaker immunoactivity	895:929	significantly weaker immunoactivity	895:929	Furthermore, de-glycosylated 30CYGP had significantly weaker immunoactivity than 30CYGP.					
31647243	8	9	dep	xylose	793:798	arg1	XM					801:802	XM	801:802	XM: 40%	801:807	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	8	10	located	observed	740:747	arg2	N-glycans					725:733	Six 30CYGP N-glycans	714:733	Six 30CYGP N-glycans	714:733	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	8	10	located	observed	740:747	arg1	total					752:756	total	752:756	total	752:756	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	11	11	from	research	1035:1042	arg1	role					1051:1054	the role	1047:1054	the role of varied glycosylation patterns in immunoactivity	1047:1105	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	7	12	gly	de-glycosylated	646:660	arg1	30CYGP					662:667	de-glycosylated 30CYGP	646:667	de-glycosylated 30CYGP	646:667	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	2	13	theme	Chinese	133:139	arg1	yam					141:143	Chinese yam	133:143	Chinese yam	133:143	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	9	14	theme	de-glycosylated	868:882	arg1	30CYGP					884:889	de-glycosylated 30CYGP	868:889	de-glycosylated 30CYGP	868:889	Furthermore, de-glycosylated 30CYGP had significantly weaker immunoactivity than 30CYGP.					
31647243	4	15	theme	30CYGP	294:299	arg1	structure					281:289	structure	281:289	structure	281:289	Research has identified the sequence and structure of 30CYGP; however, 30CYGP N-glycoform composition and immunoactivity remain unknown.					
31647243	4	15	theme	30CYGP	294:299	arg1	sequence					268:275	sequence	268:275	sequence	268:275	Research has identified the sequence and structure of 30CYGP; however, 30CYGP N-glycoform composition and immunoactivity remain unknown.					
31647243	11	16	theme	varied	1059:1064	arg1	patterns					1080:1087	varied glycosylation patterns	1059:1087	varied glycosylation patterns	1059:1087	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	7	17	theme	30CYGP	635:640	arg1	immunoactivities					615:630	the immunoactivities	611:630	the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line	611:693	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	3	18	theme	immunomodulatory	211:226	arg1	activities					228:237	strong immunomodulatory activities	204:237	strong immunomodulatory activities	204:237	glycoprotein, 30CYGP, exhibits strong immunomodulatory activities.					
31647243	7	19	theme	de-glycosylated	646:660	arg1	30CYGP					662:667	de-glycosylated 30CYGP	646:667	de-glycosylated 30CYGP	646:667	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	8	20	dep	XM	801:802	arg1	%					807:807	40%	805:807	XM: 40%	801:807	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	8	21	dep	XFM	844:846	arg1	%					851:851	60%	849:851	XFM: 60%	844:851	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	10	22	theme	30CYGP	1004:1009	arg1	immunoactivity					1011:1024	30CYGP immunoactivity	1004:1024	30CYGP immunoactivity	1004:1024	This study demonstrated that novel N-glycoforms may enhance 30CYGP immunoactivity.					
31647243	0	23	theme	N-Glycoforms	16:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam	0:61	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	0	24	theme	Immunoactivity	33:46	arg1	Analysis					0:7	Analysis	0:7	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam	0:61	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	8	25	theme	30CYGP	718:723	arg1	N-glycans					725:733	Six 30CYGP N-glycans	714:733	Six 30CYGP N-glycans	714:733	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	2	26	theme	opposita	156:163	arg1	Thunb					165:169	Dioscorea opposita Thunb.	146:170	Dioscorea opposita Thunb.	146:170	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	2	26	theme	opposita	156:163	arg1	protein					122:128	The major protein	112:128	The major protein in Chinese yam (Dioscorea opposita Thunb.)	112:171	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	11	27	theme	glycosylation	1066:1078	arg1	patterns					1080:1087	varied glycosylation patterns	1059:1087	varied glycosylation patterns	1059:1087	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	2	28	from	protein	122:128	arg1	yam					141:143	Chinese yam	133:143	Chinese yam	133:143	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	2	29	theme	Dioscorea	146:154	arg1	Thunb					165:169	Dioscorea opposita Thunb.	146:170	Dioscorea opposita Thunb.	146:170	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	2	29	theme	Dioscorea	146:154	arg1	protein					122:128	The major protein	112:128	The major protein in Chinese yam (Dioscorea opposita Thunb.)	112:171	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	9	30	gly	de-glycosylated	868:882	arg1	30CYGP					884:889	de-glycosylated 30CYGP	868:889	de-glycosylated 30CYGP	868:889	Furthermore, de-glycosylated 30CYGP had significantly weaker immunoactivity than 30CYGP.					
31647243	11	31	theme	patterns	1080:1087	arg1	role					1051:1054	the role	1047:1054	the role of varied glycosylation patterns in immunoactivity	1047:1105	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	6	32	theme	online	583:588	arg1	LC-MS					590:594	online LC-MS	583:594	online LC-MS	583:594	The N-glycans were labeled with 1-phenyl-3-methyl-5-pyrazolone and analyzed via ESI-MS and online LC-MS.					
31647243	0	33	theme	Yam	59:61	arg1	Immunoactivity					33:46	Immunoactivity	33:46	Immunoactivity	33:46	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	0	33	theme	Yam	59:61	arg1	N-Glycoforms					16:27	N-Glycoforms	16:27	N-Glycoforms	16:27	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	4	34	theme	N-glycoform	318:328	arg1	composition					330:340	30CYGP N-glycoform composition	311:340	30CYGP N-glycoform composition	311:340	Research has identified the sequence and structure of 30CYGP; however, 30CYGP N-glycoform composition and immunoactivity remain unknown.					
31647243	8	35	mod	modified	779:786	arg3	xylose					793:798	xylose	793:798	xylose (XM: 40%)	793:808	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	8	35	mod	modified	779:786	arg3	three					814:818	three	814:818	three	814:818	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	8	35	mod	modified	779:786	arg1	three					768:772	three	768:772	three	768:772	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	0	36	dep	N-Glycoforms	16:27	arg1	the					12:14	the	12:14	the	12:14	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	3	37	gly	glycoprotein	173:184	arg1	30CYGP					187:192	30CYGP	187:192	30CYGP	187:192	glycoprotein, 30CYGP, exhibits strong immunomodulatory activities.					
31647243	3	37	gly	glycoprotein	173:184	arg1	glycoprotein					173:184	glycoprotein	173:184	glycoprotein	173:184	glycoprotein, 30CYGP, exhibits strong immunomodulatory activities.					
31647243	7	38	theme	cell	685:688	arg1	line					690:693	the RAW264.7 cell line	672:693	the RAW264.7 cell line	672:693	Additionally, the immunoactivities of 30CYGP and de-glycosylated 30CYGP in the RAW264.7 cell line were investigated.					
31647243	10	39	theme	novel	973:977	arg1	N-glycoforms					979:990	novel N-glycoforms	973:990	novel N-glycoforms	973:990	This study demonstrated that novel N-glycoforms may enhance 30CYGP immunoactivity.					
31647243	8	40	dep	three	814:818	arg1	XFM					844:846	XFM	844:846	XFM: 60%	844:851	Six 30CYGP N-glycans were observed in total, in which three were modified with xylose (XM: 40%) and three with xylose and fucose (XFM: 60%).					
31647243	11	41	from	role	1051:1054	arg1	immunoactivity					1092:1105	immunoactivity	1092:1105	immunoactivity	1092:1105	Further research on the role of varied glycosylation patterns in immunoactivity is needed.					
31647243	0	42	theme	Chinese	51:57	arg1	Yam					59:61	Chinese Yam	51:61	Chinese Yam	51:61	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	2	43	theme	major	116:120	arg1	Thunb					165:169	Dioscorea opposita Thunb.	146:170	Dioscorea opposita Thunb.	146:170	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	2	43	theme	major	116:120	arg1	protein					122:128	The major protein	112:128	The major protein in Chinese yam (Dioscorea opposita Thunb.)	112:171	The major protein in Chinese yam (Dioscorea opposita Thunb.)					
31647243	0	44	dep	Analysis	0:7	arg1	Thunb					83:87	Thunb	83:87	Thunb	83:87	Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.)					
31647243	4	45	theme	30CYGP	311:316	arg1	composition					330:340	30CYGP N-glycoform composition	311:340	30CYGP N-glycoform composition	311:340	Research has identified the sequence and structure of 30CYGP; however, 30CYGP N-glycoform composition and immunoactivity remain unknown.					
29760474	0	0	theme	Endoglycosidase	85:99	arg1	EndoS					104:108	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	5	1	theme	endoglycosidases	1132:1147	arg1	bioengineering					1114:1127	the bioengineering	1110:1127	the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies	1110:1211	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	0	2	theme	S.	82:83	arg1	EndoS					104:108	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	3	3	theme	complex-type	737:748	arg1	antennae					766:773	the complex-type N-linked glycan antennae	733:773	the complex-type N-linked glycan antennae near the active site	733:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	0	4	theme	N-acetylglucosamine	243:261	arg1	residues					263:270	the first two N-acetylglucosamine residues	229:270	the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	229:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	4	theme	N-acetylglucosamine	243:261	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	5	5	theme	related	961:967	arg1	endoglycosidases					969:984	related endoglycosidases	961:984	related endoglycosidases	961:984	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	3	6	theme	well-defined	684:695	arg1	grooves					708:714	two well-defined asymmetric grooves	680:714	two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site	680:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	5	7	theme	monoclonal	1191:1200	arg1	antibodies					1202:1211	monoclonal antibodies	1191:1211	monoclonal antibodies	1191:1211	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	1	8	theme	chemoenzymatic	359:372	arg1	synthesis					374:382	the chemoenzymatic synthesis	355:382	the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties	355:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	0	9	theme	β-1,4	207:211	arg1	linkage					213:219	the β-1,4 linkage	203:219	the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	203:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	4	10	theme	glycan	821:826	arg1	site					836:839	the glycan binding site	817:839	the glycan binding site	817:839	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	0	11	theme	S	101:101	arg1	EndoS					104:108	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	3	12	theme	active	784:789	arg1	site					791:794	the active site	780:794	the active site	780:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	2	13	theme	oligosaccharide	612:626	arg1	product					631:637	its oligosaccharide G2 product	608:637	its oligosaccharide G2 product	608:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	1	14	with	antibodies	414:423	arg1	properties					451:460	improved therapeutic properties	430:460	improved therapeutic properties	430:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	5	15	theme	distinct-binding	995:1010	arg1	architectures					1017:1029	distinct-binding site architectures	995:1029	distinct-binding site architectures that correlate with the respective glycan specificities	995:1085	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	5	16	theme	antibodies	1202:1211	arg1	synthesis					1178:1186	the chemoenzymatic synthesis	1159:1186	the chemoenzymatic synthesis of monoclonal antibodies	1159:1211	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	5	17	theme	respective	1055:1064	arg1	specificities					1073:1085	the respective glycan specificities	1051:1085	the respective glycan specificities	1051:1085	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	0	18	theme	linkage	213:219	arg1	hydrolysis					189:198	the hydrolysis	185:198	the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	185:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	19	theme	biantennary	279:289	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	4	20	theme	binding	828:834	arg1	site					836:839	the glycan binding site	817:839	the glycan binding site	817:839	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	1	21	theme	glycosylated	401:412	arg1	antibodies					414:423	homogeneously glycosylated antibodies	387:423	homogeneously glycosylated antibodies with improved therapeutic properties	387:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	0	22	theme	first	233:237	arg1	residues					263:270	the first two N-acetylglucosamine residues	229:270	the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	229:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	22	theme	first	233:237	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	4	23	theme	Several	797:803	arg1	loops					805:809	Several loops	797:809	Several loops	797:809	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	3	24	theme	N-linked	750:757	arg1	antennae					766:773	the complex-type N-linked glycan antennae	733:773	the complex-type N-linked glycan antennae near the active site	733:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	0	25	theme	Structural	0:9	arg1	endo-β-N-acetylglucosaminidase					126:155	a bacterial endo-β-N-acetylglucosaminidase	114:155	a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	114:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	25	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS)	0:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	26	theme	N-linked	304:311	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	5	27	theme	loops	938:942	arg1	arrangement					917:927	the arrangement	913:927	the arrangement of these loops within EndoS and related endoglycosidases	913:984	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	2	28	theme	EndoS	586:590	arg1	structure					557:565	the crystal structure	545:565	the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product	545:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	0	29	theme	complex-type	291:302	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	1	30	theme	molecular	471:479	arg1	basis					481:485	the molecular basis	467:485	the molecular basis for its substrate specificity	467:515	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	1	30	theme	molecular	471:479	arg1	unknown					520:526	unknown	520:526	unknown	520:526	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	2	31	theme	full-length	574:584	arg1	EndoS					586:590	the full-length EndoS	570:590	the full-length EndoS	570:590	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	1	32	theme	antibodies	414:423	arg1	synthesis					374:382	the chemoenzymatic synthesis	355:382	the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties	355:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	1	33	gly	glycosylated	401:412	arg1	antibodies					414:423	homogeneously glycosylated antibodies	387:423	homogeneously glycosylated antibodies with improved therapeutic properties	387:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	3	34	contain	contains	671:678	arg2	grooves					708:714	two well-defined asymmetric grooves	680:714	two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site	680:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	3	34	contain	contains	671:678	arg1	domain					664:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	0	35	link	N-linked	304:311	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	3	36	theme	asymmetric	697:706	arg1	grooves					708:714	two well-defined asymmetric grooves	680:714	two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site	680:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	4	37	theme	substrate	871:879	arg1	specificity					881:891	the strict substrate specificity	860:891	the strict substrate specificity of EndoS	860:900	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	0	38	theme	bacterial	116:124	arg1	endo-β-N-acetylglucosaminidase					126:155	a bacterial endo-β-N-acetylglucosaminidase	114:155	a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	114:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	38	theme	bacterial	116:124	arg1	basis					11:15	Structural basis	0:15	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS)	0:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	1	39	theme	improved	430:437	arg1	properties					451:460	improved therapeutic properties	430:460	improved therapeutic properties	430:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	4	40	theme	strict	864:869	arg1	specificity					881:891	the strict substrate specificity	860:891	the strict substrate specificity of EndoS	860:900	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	0	41	theme	N-glycans	53:61	arg1	recognition					25:35	the recognition	21:35	the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS)	21:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	1	42	theme	therapeutic	439:449	arg1	properties					451:460	improved therapeutic properties	430:460	improved therapeutic properties	430:460	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	0	43	theme	glycans	313:319	arg1	residues					263:270	the first two N-acetylglucosamine residues	229:270	the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions	229:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	43	theme	glycans	313:319	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	44	theme	complex-type	40:51	arg1	N-glycans					53:61	complex-type N-glycans	40:61	complex-type N-glycans	40:61	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	4	45	theme	EndoS	896:900	arg1	specificity					881:891	the strict substrate specificity	860:891	the strict substrate specificity of EndoS	860:900	Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS.					
29760474	0	46	theme	Fc	328:329	arg1	regions					331:337	IgG Fc regions	324:337	IgG Fc regions	324:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	2	47	theme	G2	628:629	arg1	product					631:637	its oligosaccharide G2 product	608:637	its oligosaccharide G2 product	608:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	1	48	used	used	346:349	arg2	It					340:341	It	340:341	It	340:341	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	5	49	theme	site	1012:1015	arg1	architectures					1017:1029	distinct-binding site architectures	995:1029	distinct-binding site architectures that correlate with the respective glycan specificities	995:1085	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	1	50	theme	substrate	495:503	arg1	specificity					505:515	its substrate specificity	491:515	its substrate specificity	491:515	It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown.					
29760474	3	51	theme	hydrolase	654:662	arg1	domain					664:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	0	52	theme	IgG	324:326	arg1	regions					331:337	IgG Fc regions	324:337	IgG Fc regions	324:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	5	53	theme	glycan	1066:1071	arg1	specificities					1073:1085	the respective glycan specificities	1051:1085	the respective glycan specificities	1051:1085	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	2	54	from	structure	557:565	arg1	complex					595:601	complex	595:601	complex with its oligosaccharide G2 product	595:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	5	55	dep	reveals	987:993	arg1	Comparing					903:911	Comparing	903:911	Comparing the arrangement of these loops within EndoS and related endoglycosidases	903:984	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	5	56	theme	chemoenzymatic	1163:1176	arg1	synthesis					1178:1186	the chemoenzymatic synthesis	1159:1186	the chemoenzymatic synthesis of monoclonal antibodies	1159:1211	Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.					
29760474	3	57	link	N-linked	750:757	arg1	antennae					766:773	the complex-type N-linked glycan antennae	733:773	the complex-type N-linked glycan antennae near the active site	733:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	0	58	theme	Endoglycosidase	66:80	arg1	EndoS					104:108	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Endoglycosidase S. Endoglycosidase S (EndoS)	66:109	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	0	59	theme	regions	331:337	arg1	glycans					313:319	the biantennary complex-type N-linked glycans	275:319	the biantennary complex-type N-linked glycans of IgG Fc regions	275:337	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Endoglycosidase S (EndoS) is a bacterial endo-β-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the β-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions.					
29760474	3	60	theme	glycan	759:764	arg1	antennae					766:773	the complex-type N-linked glycan antennae	733:773	the complex-type N-linked glycan antennae near the active site	733:794	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	3	61	theme	glycoside	644:652	arg1	domain					664:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain	640:669	The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site.					
29760474	2	62	with	complex	595:601	arg1	product					631:637	its oligosaccharide G2 product	608:637	its oligosaccharide G2 product	608:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
29760474	2	63	theme	crystal	549:555	arg1	structure					557:565	the crystal structure	545:565	the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product	545:637	Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product.					
30900906	2	0	theme	O-	412:413	arg1	range					403:407	a wide range	396:407	a wide range of O-, N-, and C-nucleophiles	396:437	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	3	1	theme	-glucosamino	588:599	arg1	residues					601:608	β-d-(1→3)-glucosamino residues	579:608	β-d-(1→3)-glucosamino residues	579:608	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	1	2	gly	glycosylation	316:328	arg1	ortho-hexynylbenzoates					342:363	glycosyl ortho-hexynylbenzoates	333:363	glycosyl ortho-hexynylbenzoates	333:363	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	2	3	from	features	443:450	arg1	yield					501:505	high yield	496:505	high yield	496:505	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	1	4	theme	efficient	116:124	arg1	protocol					126:133	An efficient protocol	113:133	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages	113:183	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	2	5	theme	wide	398:401	arg1	range					403:407	a wide range	396:407	a wide range of O-, N-, and C-nucleophiles	396:437	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	2	6	theme	high	496:499	arg1	yield					501:505	high yield	496:505	high yield	496:505	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	0	7	theme	SN2-Type	39:46	arg1	Reaction					61:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction Enables Synthesis of β-d-Glycosaminosides.					
30900906	3	8	theme	Oligomerization-ready	530:550	arg1	trisaccharide					552:564	Oligomerization-ready trisaccharide	530:564	Oligomerization-ready trisaccharide	530:564	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	0	9	theme	2,4-Dinitrobenzenesulfonamide-Directed	0:37	arg1	Reaction					61:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction Enables Synthesis of β-d-Glycosaminosides.					
30900906	1	10	theme	-directed	297:305	arg1	glycosylation					316:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation	228:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates	228:363	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	2	11	theme	N-	416:417	arg1	range					403:407	a wide range	396:407	a wide range of O-, N-, and C-nucleophiles	396:437	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	1	12	theme	SN2-like	307:314	arg1	glycosylation					316:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation	228:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates	228:363	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	0	13	theme	Displacement	48:59	arg1	Reaction					61:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction	0:68	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction Enables Synthesis of β-d-Glycosaminosides.					
30900906	3	14	dep	Neisseria	711:719	arg1	meningitidis					721:732	meningitidis	721:732	meningitidis	721:732	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	2	15	theme	mild	513:516	arg1	conditions					518:527	mild conditions	513:527	mild conditions	513:527	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	3	16	theme	Neisseria	711:719	arg1	polysaccharide					743:756	Neisseria meningitidis capsular polysaccharide	711:756	Neisseria meningitidis capsular polysaccharide	711:756	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	1	17	theme	glycosyl	333:340	arg1	ortho-hexynylbenzoates					342:363	glycosyl ortho-hexynylbenzoates	333:363	glycosyl ortho-hexynylbenzoates	333:363	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	0	18	theme	β-d-Glycosaminosides	91:110	arg1	Synthesis					78:86	Synthesis	78:86	Synthesis of β-d-Glycosaminosides	78:110	2,4-Dinitrobenzenesulfonamide-Directed SN2-Type Displacement Reaction Enables Synthesis of β-d-Glycosaminosides.					
30900906	3	19	theme	oligosaccharides	678:693	arg1	synthesis					665:673	the synthesis	661:673	the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide	661:756	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	2	20	theme	convenient	452:461	arg1	conversion					463:472	convenient conversion	452:472	convenient conversion of DNsNH into AcNH	452:491	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	1	21	dep	protocol	126:133	arg1	construct					138:146	construct	138:146	to construct β-d-gluco-/galactosaminosyl linkages	135:183	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	1	22	theme	β-d-gluco-/galactosaminosyl	148:174	arg1	linkages					176:183	β-d-gluco-/galactosaminosyl linkages	148:183	β-d-gluco-/galactosaminosyl linkages	148:183	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	1	23	theme	ortho-hexynylbenzoates	342:363	arg1	glycosylation					316:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation	228:328	strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates	228:363	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	1	23	theme	ortho-hexynylbenzoates	342:363	arg1	nonparticipating					207:222	nonparticipating	207:222	nonparticipating	207:222	An efficient protocol to construct β-d-gluco-/galactosaminosyl linkages was established using nonparticipating and strong electron-withdrawing C-2-2,4-dinitrobenzenesulfonamide (DNsNH)-directed SN2-like glycosylation of glycosyl ortho-hexynylbenzoates.					
30900906	3	24	theme	solid	640:644	arg1	foundation					646:655	a solid foundation	638:655	a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide	638:756	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	2	25	theme	C-nucleophiles	424:437	arg1	range					403:407	a wide range	396:407	a wide range of O-, N-, and C-nucleophiles	396:437	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30900906	3	26	theme	capsular	734:741	arg1	polysaccharide					743:756	Neisseria meningitidis capsular polysaccharide	711:756	Neisseria meningitidis capsular polysaccharide	711:756	Oligomerization-ready trisaccharide, composed of β-d-(1→3)-glucosamino residues, has been achieved, setting a solid foundation for the synthesis of oligosaccharides associated with Neisseria meningitidis capsular polysaccharide.					
30900906	2	27	theme	DNsNH	477:481	arg1	conversion					463:472	convenient conversion	452:472	convenient conversion of DNsNH into AcNH	452:491	The reaction is applicable to a wide range of O-, N-, and C-nucleophiles and features convenient conversion of DNsNH into AcNH in high yield under mild conditions.					
30612271	14	0	from	enrichment	1778:1787	arg1	tissues					1824:1830	tissues	1824:1830	tissues	1824:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	0	from	enrichment	1778:1787	arg1	urine					1815:1819	urine	1815:1819	urine	1815:1819	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	0	from	enrichment	1778:1787	arg1	plasma					1807:1812	plasma	1807:1812	plasma	1807:1812	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	1	1	theme	lipoxidation	232:243	arg1	ALEs					259:262	ALEs	259:262	ALEs	259:262	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	1	theme	lipoxidation	232:243	arg1	products					249:256	advanced lipoxidation end products	223:256	advanced lipoxidation end products (ALEs)	223:263	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	3	2	theme	transmembrane	484:496	arg1	glycoprotein					498:509	a type I transmembrane glycoprotein	475:509	a type I transmembrane glycoprotein	475:509	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	3	2	theme	transmembrane	484:496	arg1	RAGE					469:472	RAGE	469:472	RAGE	469:472	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	12	3	theme	Thermal	1373:1379	arg1	curves					1394:1399	Thermal denaturation curves	1373:1399	Thermal denaturation curves	1373:1399	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	11	4	theme	dichroism	1271:1279	arg1	spectra					1281:1287	Circular dichroism spectra	1262:1287	Circular dichroism spectra	1262:1287	Circular dichroism spectra indicated that VC1 maintains the same conformation also after removal of N-glycans.					
30612271	13	5	theme	moiety	1523:1528	arg1	removal					1501:1507	The removal	1497:1507	The removal of the glycan moiety	1497:1528	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	7	6	theme	N-glycosylation	773:787	arg1	sites					789:793	two N-glycosylation sites	769:793	two N-glycosylation sites	769:793	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	5	7	theme	ligands	658:664	arg1	majority					641:648	The majority	637:648	The majority of RAGE ligands	637:664	The majority of RAGE ligands bind to the VC1 domain.					
30612271	14	8	theme	RAGE	1664:1667	arg1	role					1656:1659	the crucial role	1644:1659	the crucial role of RAGE in human pathologies	1644:1688	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	9	from	tissues	1824:1830	arg1	AGEs/ALEs					1792:1800	AGEs/ALEs	1792:1800	AGEs/ALEs from plasma, urine or tissues	1792:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	9	from	tissues	1824:1830	arg1	enrichment					1778:1787	the enrichment	1774:1787	the enrichment of AGEs/ALEs from plasma, urine or tissues	1774:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	4	10	theme	oxidative	593:601	arg1	diseases					616:623	oxidative stress-based diseases	593:623	oxidative stress-based diseases	593:623	RAGE is involved in chronic inflammation, oxidative stress-based diseases and ageing.					
30612271	14	11	dep	useful	1739:1744	arg1	characterizing					1840:1853	characterizing	1840:1853	characterizing the nature of the interaction	1840:1883	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	11	dep	useful	1739:1744	arg1	designing					1749:1757	designing	1749:1757	designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues	1749:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	0	12	theme	advanced	112:119	arg1	products					135:142	advanced glycation end products	112:142	advanced glycation end products (RAGE)	112:149	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	12	theme	advanced	112:119	arg1	RAGE					145:148	RAGE	145:148	RAGE	145:148	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	1	13	theme	glycation	189:197	arg1	AGEs					213:216	AGEs	213:216	AGEs	213:216	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	13	theme	glycation	189:197	arg1	products					203:210	Advanced glycation end products	180:210	Advanced glycation end products (AGEs)	180:217	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	0	14	theme	end	131:133	arg1	products					135:142	advanced glycation end products	112:142	advanced glycation end products (RAGE)	112:149	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	14	theme	end	131:133	arg1	RAGE					145:148	RAGE	145:148	RAGE	145:148	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	14	15	theme	human	1672:1676	arg1	pathologies					1678:1688	human pathologies	1672:1688	human pathologies	1672:1688	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	1	16	theme	non-enzymatic	281:293	arg1	modifications					295:307	non-enzymatic modifications	281:307	non-enzymatic modifications of proteins	281:319	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	8	17	from	properties	975:984	arg1	Analysis					923:930	Analysis	923:930	Analysis of N-glycans and of their influence on VC1 properties	923:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	2	18	theme	receptor-mediated	439:455	arg1	mechanisms					457:466	receptor-mediated mechanisms	439:466	receptor-mediated mechanisms	439:466	They directly act on proteins, affecting structure and function, or through receptor-mediated mechanisms.					
30612271	7	19	gly	N-glycosylation	773:787	arg2	sites					789:793	two N-glycosylation sites	769:793	two N-glycosylation sites	769:793	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	7	19	gly	N-glycosylation	773:787	arg2	two					769:771	two	769:771	two	769:771	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	10	20	theme	major	1185:1189	arg1	Man10GlcNAc2-					1191:1203	major Man10GlcNAc2-	1185:1203	major Man10GlcNAc2-	1185:1203	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	13	21	link	malondialdehyde-derived	1589:1611	arg1	albumin					1629:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	12	22	theme	VC1	1471:1473	arg1	conformation					1483:1494	VC1 protein conformation	1471:1494	VC1 protein conformation	1471:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	0	23	theme	domain	79:84	arg1	characteristics					52:66	the characteristics	48:66	the characteristics of the VC1 domain of the human receptor	48:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	10	24	attach	released	1120:1127	arg2	N-glycans					1110:1118	N-glycans	1110:1118	N-glycans released from VC1	1110:1136	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	10	24	attach	released	1120:1127	arg1	VC1					1134:1136	VC1	1134:1136	VC1	1134:1136	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	2	25	dep	proteins	384:391	arg1	affecting					394:402	affecting	394:402	affecting structure and function	394:425	They directly act on proteins, affecting structure and function, or through receptor-mediated mechanisms.					
30612271	1	26	theme	end	245:247	arg1	ALEs					259:262	ALEs	259:262	ALEs	259:262	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	26	theme	end	245:247	arg1	products					249:256	advanced lipoxidation end products	223:256	advanced lipoxidation end products (ALEs)	223:263	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	12	27	theme	stabilizing	1449:1459	arg1	effect					1461:1466	a small stabilizing effect	1441:1466	a small stabilizing effect on VC1 protein conformation	1441:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	13	28	theme	ALE-human	1613:1621	arg1	albumin					1629:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	9	29	used	used	1081:1084	arg2	method					1053:1058	The highly sensitive procainamide labeling method	1010:1058	The highly sensitive procainamide labeling method coupled to ES-MS	1010:1075	The highly sensitive procainamide labeling method coupled to ES-MS was used for N-glycan profiling.					
30612271	9	30	theme	sensitive	1021:1029	arg1	method					1053:1058	The highly sensitive procainamide labeling method	1010:1058	The highly sensitive procainamide labeling method coupled to ES-MS	1010:1075	The highly sensitive procainamide labeling method coupled to ES-MS was used for N-glycan profiling.					
30612271	13	31	theme	VC1	1560:1562	arg1	binding					1549:1555	the binding	1545:1555	the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin	1545:1635	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	9	32	theme	labeling	1044:1051	arg1	method					1053:1058	The highly sensitive procainamide labeling method	1010:1058	The highly sensitive procainamide labeling method coupled to ES-MS	1010:1075	The highly sensitive procainamide labeling method coupled to ES-MS was used for N-glycan profiling.					
30612271	14	33	from	pastoris	1719:1726	arg1	features					1695:1702	the features	1691:1702	the features of VC1 from P. pastoris	1691:1726	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	10	34	with	Man15GlcNAc2-	1166:1178	arg1	species					1223:1229	Man11GlcNAc2- species	1209:1229	Man11GlcNAc2- species	1209:1229	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	10	34	with	Man15GlcNAc2-	1166:1178	arg1	Man10GlcNAc2-					1191:1203	major Man10GlcNAc2-	1185:1203	major Man10GlcNAc2-	1185:1203	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	14	35	theme	interaction	1873:1883	arg1	nature					1859:1864	the nature	1855:1864	the nature of the interaction	1855:1883	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	13	36	theme	sugar-derived	1567:1579	arg1	AGE-					1581:1584	sugar-derived AGE-	1567:1584	sugar-derived AGE-	1567:1584	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	14	37	theme	VC1	1707:1709	arg1	features					1695:1702	the features	1691:1702	the features of VC1 from P. pastoris	1691:1726	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	0	38	gly	N-glycosylation	29:43	arg1	receptor					99:106	the human receptor	89:106	the human receptor	89:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	38	gly	N-glycosylation	29:43	arg1	domain					79:84	the VC1 domain	71:84	the VC1 domain of the human receptor	71:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	3	39	gly	glycoprotein	498:509	arg1	glycoprotein					498:509	a type I transmembrane glycoprotein	475:509	a type I transmembrane glycoprotein	475:509	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	3	39	gly	glycoprotein	498:509	arg1	RAGE					469:472	RAGE	469:472	RAGE	469:472	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	11	40	theme	same	1322:1325	arg1	conformation					1327:1338	the same conformation	1318:1338	the same conformation	1318:1338	Circular dichroism spectra indicated that VC1 maintains the same conformation also after removal of N-glycans.					
30612271	7	41	used	occupied	818:825	arg2	Asn25					801:805	Asn25	801:805	Asn25	801:805	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	7	41	used	occupied	818:825	arg2	one					796:798	one	796:798	one	796:798	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	0	42	theme	Pichia	163:168	arg1	pastoris					170:177	Pichia pastoris	163:177	Pichia pastoris	163:177	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	9	43	theme	N-glycan	1090:1097	arg1	profiling					1099:1107	N-glycan profiling	1090:1107	N-glycan profiling	1090:1107	The highly sensitive procainamide labeling method coupled to ES-MS was used for N-glycan profiling.					
30612271	1	44	theme	advanced	223:230	arg1	ALEs					259:262	ALEs	259:262	ALEs	259:262	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	44	theme	advanced	223:230	arg1	products					249:256	advanced lipoxidation end products	223:256	advanced lipoxidation end products (ALEs)	223:263	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	6	45	theme	Pichia	745:750	arg1	pastoris					752:759	Pichia pastoris	745:759	Pichia pastoris	745:759	This domain was successfully expressed and secreted by Pichia pastoris.					
30612271	3	46	theme	I	482:482	arg1	glycoprotein					498:509	a type I transmembrane glycoprotein	475:509	a type I transmembrane glycoprotein	475:509	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	3	46	theme	I	482:482	arg1	RAGE					469:472	RAGE	469:472	RAGE	469:472	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	12	47	theme	denaturation	1381:1392	arg1	curves					1394:1399	Thermal denaturation curves	1373:1399	Thermal denaturation curves	1373:1399	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	4	48	theme	stress-based	603:614	arg1	diseases					616:623	oxidative stress-based diseases	593:623	oxidative stress-based diseases	593:623	RAGE is involved in chronic inflammation, oxidative stress-based diseases and ageing.					
30612271	11	49	theme	Circular	1262:1269	arg1	spectra					1281:1287	Circular dichroism spectra	1262:1287	Circular dichroism spectra	1262:1287	Circular dichroism spectra indicated that VC1 maintains the same conformation also after removal of N-glycans.					
30612271	13	50	theme	glycan	1516:1521	arg1	moiety					1523:1528	the glycan moiety	1512:1528	the glycan moiety	1512:1528	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	12	51	from	effect	1461:1466	arg1	conformation					1483:1494	VC1 protein conformation	1471:1494	VC1 protein conformation	1471:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	5	52	theme	RAGE	653:656	arg1	ligands					658:664	RAGE ligands	653:664	RAGE ligands	653:664	The majority of RAGE ligands bind to the VC1 domain.					
30612271	14	53	from	urine	1815:1819	arg1	AGEs/ALEs					1792:1800	AGEs/ALEs	1792:1800	AGEs/ALEs from plasma, urine or tissues	1792:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	53	from	urine	1815:1819	arg1	enrichment					1778:1787	the enrichment	1774:1787	the enrichment of AGEs/ALEs from plasma, urine or tissues	1774:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	0	54	theme	glycation	121:129	arg1	products					135:142	advanced glycation end products	112:142	advanced glycation end products (RAGE)	112:149	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	54	theme	glycation	121:129	arg1	RAGE					145:148	RAGE	145:148	RAGE	145:148	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	1	55	theme	human	349:353	arg1	health					355:360	human health	349:360	human health	349:360	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	56	theme	Advanced	180:187	arg1	AGEs					213:216	AGEs	213:216	AGEs	213:216	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	56	theme	Advanced	180:187	arg1	products					203:210	Advanced glycation end products	180:210	Advanced glycation end products (AGEs)	180:217	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	14	57	theme	crucial	1648:1654	arg1	role					1656:1659	the crucial role	1644:1659	the crucial role of RAGE in human pathologies	1644:1688	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	13	58	link	sugar-derived	1567:1579	arg1	AGE-					1581:1584	sugar-derived AGE-	1567:1584	sugar-derived AGE-	1567:1584	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	8	59	from	influence	958:966	arg1	properties					975:984	VC1 properties	971:984	VC1 properties	971:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	14	60	from	role	1656:1659	arg1	pathologies					1678:1688	human pathologies	1672:1688	human pathologies	1672:1688	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	12	61	contain	has	1437:1439	arg2	effect					1461:1466	a small stabilizing effect	1441:1466	a small stabilizing effect on VC1 protein conformation	1441:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	12	61	contain	has	1437:1439	arg1	moiety					1430:1435	the carbohydrate moiety	1413:1435	the carbohydrate moiety	1413:1435	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	10	62	dep	Man15GlcNAc2-	1166:1178	arg1	Man9GlcNAc2-					1150:1161	Man9GlcNAc2-	1150:1161	Man9GlcNAc2-	1150:1161	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	14	63	theme	AGEs/ALEs	1792:1800	arg1	enrichment					1778:1787	the enrichment	1774:1787	the enrichment of AGEs/ALEs from plasma, urine or tissues	1774:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	0	64	from	effects	18:24	arg1	characteristics					52:66	the characteristics	48:66	the characteristics of the VC1 domain of the human receptor	48:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	1	65	theme	end	199:201	arg1	AGEs					213:216	AGEs	213:216	AGEs	213:216	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	1	65	theme	end	199:201	arg1	products					203:210	Advanced glycation end products	180:210	Advanced glycation end products (AGEs)	180:217	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	5	66	theme	VC1	678:680	arg1	domain					682:687	the VC1 domain	674:687	the VC1 domain	674:687	The majority of RAGE ligands bind to the VC1 domain.					
30612271	0	67	theme	VC1	75:77	arg1	receptor					99:106	the human receptor	89:106	the human receptor	89:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	67	theme	VC1	75:77	arg1	domain					79:84	the VC1 domain	71:84	the VC1 domain of the human receptor	71:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	8	68	theme	N-glycans	935:943	arg1	Analysis					923:930	Analysis	923:930	Analysis of N-glycans and of their influence on VC1 properties	923:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	12	69	theme	protein	1475:1481	arg1	conformation					1483:1494	VC1 protein conformation	1471:1494	VC1 protein conformation	1471:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	0	70	theme	receptor	99:106	arg1	receptor					99:106	the human receptor	89:106	the human receptor	89:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	0	70	theme	receptor	99:106	arg1	domain					79:84	the VC1 domain	71:84	the VC1 domain of the human receptor	71:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	1	71	theme	proteins	312:319	arg1	modifications					295:307	non-enzymatic modifications	281:307	non-enzymatic modifications of proteins	281:319	Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health.					
30612271	7	72	theme	VC1	890:892	arg1	variants					894:901	two VC1 variants	886:901	two VC1 variants	886:901	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	0	73	theme	human	93:97	arg1	receptor					99:106	the human receptor	89:106	the human receptor	89:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	8	74	theme	influence	958:966	arg1	Analysis					923:930	Analysis	923:930	Analysis of N-glycans and of their influence on VC1 properties	923:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	4	75	theme	chronic	571:577	arg1	inflammation					579:590	chronic inflammation	571:590	chronic inflammation	571:590	RAGE is involved in chronic inflammation, oxidative stress-based diseases and ageing.					
30612271	10	76	theme	Man11GlcNAc2-	1209:1221	arg1	species					1223:1229	Man11GlcNAc2- species	1209:1229	Man11GlcNAc2- species	1209:1229	N-glycans released from VC1 ranged from Man9GlcNAc2- to Man15GlcNAc2- with major Man10GlcNAc2- and Man11GlcNAc2- species for p36 and p34, respectively.					
30612271	8	77	theme	VC1	971:973	arg1	properties					975:984	VC1 properties	971:984	VC1 properties	971:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	13	78	theme	malondialdehyde-derived	1589:1611	arg1	albumin					1629:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	0	79	theme	N-glycosylation	29:43	arg1	effects					18:24	the effects	14:24	the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor	14:106	Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.					
30612271	12	80	theme	small	1443:1447	arg1	effect					1461:1466	a small stabilizing effect	1441:1466	a small stabilizing effect on VC1 protein conformation	1441:1494	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	13	81	theme	serum	1623:1627	arg1	albumin					1629:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	malondialdehyde-derived ALE-human serum albumin	1589:1635	The removal of the glycan moiety did not affect the binding of VC1 to sugar-derived AGE- or malondialdehyde-derived ALE-human serum albumin.					
30612271	9	82	theme	procainamide	1031:1042	arg1	method					1053:1058	The highly sensitive procainamide labeling method	1010:1058	The highly sensitive procainamide labeling method coupled to ES-MS	1010:1075	The highly sensitive procainamide labeling method coupled to ES-MS was used for N-glycan profiling.					
30612271	12	83	theme	carbohydrate	1417:1428	arg1	moiety					1430:1435	the carbohydrate moiety	1413:1435	the carbohydrate moiety	1413:1435	Thermal denaturation curves showed that the carbohydrate moiety has a small stabilizing effect on VC1 protein conformation.					
30612271	7	84	gly	under-glycosylated	855:872	arg1	Asn81					844:848	Asn81	844:848	Asn81	844:848	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	7	84	gly	under-glycosylated	855:872	arg1	other					837:841	other	837:841	other	837:841	Out of two N-glycosylation sites, one (Asn25) was fully occupied while the other (Asn81) was under-glycosylated, generating two VC1 variants, named p36 and p34.					
30612271	14	85	from	plasma	1807:1812	arg1	AGEs/ALEs					1792:1800	AGEs/ALEs	1792:1800	AGEs/ALEs from plasma, urine or tissues	1792:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	14	85	from	plasma	1807:1812	arg1	enrichment					1778:1787	the enrichment	1774:1787	the enrichment of AGEs/ALEs from plasma, urine or tissues	1774:1830	Given the crucial role of RAGE in human pathologies, the features of VC1 from P. pastoris will prove useful in designing strategies for the enrichment of AGEs/ALEs from plasma, urine or tissues, and in characterizing the nature of the interaction.					
30612271	11	86	theme	N-glycans	1362:1370	arg1	removal					1351:1357	removal	1351:1357	removal of N-glycans	1351:1370	Circular dichroism spectra indicated that VC1 maintains the same conformation also after removal of N-glycans.					
30612271	8	87	from	Analysis	923:930	arg1	properties					975:984	VC1 properties	971:984	VC1 properties	971:984	Analysis of N-glycans and of their influence on VC1 properties were here investigated.					
30612271	3	88	theme	type	477:480	arg1	glycoprotein					498:509	a type I transmembrane glycoprotein	475:509	a type I transmembrane glycoprotein	475:509	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
30612271	3	88	theme	type	477:480	arg1	RAGE					469:472	RAGE	469:472	RAGE	469:472	RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs.					
31111416	5	0	theme	information	802:812	arg1	gaps					814:817	occasional information gaps	791:817	occasional information gaps that could not possibly be filled by means of the automatic data analysis	791:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	8	1	theme	result	1245:1250	arg1	verification					1252:1263	the result verification	1241:1263	the result verification	1241:1263	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	10	2	dep	correlated	1621:1630	arg1	"					1665:1665	"	1665:1665	"	1665:1665	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	7	3	theme	glycan	1048:1053	arg1	sites					1055:1059	the glycan sites	1044:1059	the glycan sites	1044:1059	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	3	4	theme	trimeric	523:530	arg1	assembly					532:539	the trimeric assembly	519:539	the trimeric assembly	519:539	Another structural feature was reported, related to its cleaved subunits within the trimeric assembly.					
31111416	11	5	theme	Experimental	1667:1678	arg1	optimization					1690:1701	Experimental procedure optimization	1667:1701	Experimental procedure optimization	1667:1701	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	6	6	dep	applied	932:938	arg1	followed					941:948	followed	941:948	followed by the illustrations on how the high-quality results may be misinterpreted	941:1023	A series of data validation steps was applied, followed by the illustrations on how the high-quality results may be misinterpreted.					
31111416	2	7	gly	glycosylation	326:338	arg1	Env					377:379	Env	377:379	Env	377:379	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	9	8	dep	provided	1411:1418	arg1	whereas					1445:1451	whereas	1445:1451	whereas	1445:1451	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	1	9	theme	essential	173:181	arg1	step					183:186	the essential step	169:186	the essential step of structure-based vaccine design programs	169:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	9	theme	essential	173:181	arg1	Characterization					97:112	Characterization	97:112	Characterization of HIV Env glycoprotein with 28 glycosylation sites	97:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	5	10	theme	vast	766:769	arg1	data					781:784	data	781:784	data with occasional information gaps that could not possibly be filled by means of the automatic data analysis	781:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	5	10	theme	vast	766:769	arg1	amount					771:776	a vast amount	764:776	a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis	764:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	2	11	theme	Env	377:379	arg1	occupancy					364:372	glycosite occupancy	354:372	glycosite occupancy of Env	354:379	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	2	11	theme	Env	377:379	arg1	sequence					316:323	the primary sequence	304:323	the primary sequence	304:323	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	2	11	theme	Env	377:379	arg1	profiles					340:347	glycosylation profiles	326:347	glycosylation profiles	326:347	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	9	12	theme	Env	1545:1547	arg1	sequence					1549:1556	Env sequence	1545:1556	Env sequence	1545:1556	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	9	13	theme	individual	1427:1436	arg1	sites					1438:1442	20 individual sites	1424:1442	20 individual sites	1424:1442	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	6	14	theme	steps	922:926	arg1	series					896:901	A series	894:901	A series of data validation steps	894:926	A series of data validation steps was applied, followed by the illustrations on how the high-quality results may be misinterpreted.					
31111416	2	15	theme	primary	308:314	arg1	sequence					316:323	the primary sequence	304:323	the primary sequence	304:323	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	4	16	theme	results	609:615	arg1	inspection					591:600	thorough inspection	582:600	thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization	582:723	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
31111416	11	17	with	workflow	1709:1716	arg1	focus					1753:1757	a focus	1751:1757	a focus on stringent data testing	1751:1783	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	6	18	theme	validation	911:920	arg1	steps					922:926	data validation steps	906:926	data validation steps	906:926	A series of data validation steps was applied, followed by the illustrations on how the high-quality results may be misinterpreted.					
31111416	11	19	theme	procedure	1680:1688	arg1	optimization					1690:1701	Experimental procedure optimization	1667:1701	Experimental procedure optimization	1667:1701	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	8	20	theme	antibody-recognition	1352:1371	arg1	sites					1373:1377	3 critical antibody-recognition sites	1341:1377	3 critical antibody-recognition sites	1341:1377	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	3	21	theme	structural	447:456	arg1	feature					458:464	Another structural feature	439:464	Another structural feature	439:464	Another structural feature was reported, related to its cleaved subunits within the trimeric assembly.					
31111416	5	22	theme	data	781:784	arg1	data					781:784	data	781:784	data with occasional information gaps that could not possibly be filled by means of the automatic data analysis	781:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	5	22	theme	data	781:784	arg1	amount					771:776	a vast amount	764:776	a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis	764:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	6	23	theme	data	906:909	arg1	steps					922:926	data validation steps	906:926	data validation steps	906:926	A series of data validation steps was applied, followed by the illustrations on how the high-quality results may be misinterpreted.					
31111416	8	24	theme	critical	1343:1350	arg1	sites					1373:1377	3 critical antibody-recognition sites	1341:1377	3 critical antibody-recognition sites	1341:1377	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	10	25	theme	mannose	1651:1657	arg1	patch					1659:1663	the expected "mannose patch	1637:1663	the expected "mannose patch	1637:1663	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	7	26	theme	molecule	1170:1177	arg1	characterization					1145:1160	full structural characterization	1129:1160	full structural characterization of this molecule	1129:1177	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	11	27	theme	current	1806:1812	arg1	study					1814:1818	the current study	1802:1818	the current study	1802:1818	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	10	28	theme	unprocessed	1583:1593	arg1	glycans					1613:1619	the unprocessed high mannose-type glycans	1579:1619	the unprocessed high mannose-type glycans	1579:1619	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	1	29	theme	structure-based	191:205	arg1	programs					222:229	structure-based vaccine design programs	191:229	structure-based vaccine design programs	191:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	10	30	theme	high	1595:1598	arg1	glycans					1613:1619	the unprocessed high mannose-type glycans	1579:1619	the unprocessed high mannose-type glycans	1579:1619	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	10	31	theme	glycans	1613:1619	arg1	distribution					1563:1574	The distribution	1559:1574	The distribution of the unprocessed high mannose-type glycans	1559:1619	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	7	32	theme	certain	1092:1098	arg1	limit					1100:1104	a certain limit	1090:1104	a certain limit	1090:1104	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	11	33	with	optimization	1690:1701	arg1	focus					1753:1757	a focus	1751:1757	a focus on stringent data testing	1751:1783	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	1	34	theme	vaccine	207:213	arg1	programs					222:229	structure-based vaccine design programs	191:229	structure-based vaccine design programs	191:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	35	with	Characterization	97:112	arg1	sites					160:164	28 glycosylation sites	143:164	28 glycosylation sites	143:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	6	36	theme	high-quality	982:993	arg1	results					995:1001	the high-quality results	978:1001	the high-quality results	978:1001	A series of data validation steps was applied, followed by the illustrations on how the high-quality results may be misinterpreted.					
31111416	5	37	theme	Env	744:746	arg1	complexity					730:739	The complexity	726:739	The complexity of Env	726:746	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	2	38	theme	glycosylation	326:338	arg1	profiles					340:347	glycosylation profiles	326:347	glycosylation profiles	326:347	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	1	39	theme	design	215:220	arg1	programs					222:229	structure-based vaccine design programs	191:229	structure-based vaccine design programs	191:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	11	40	theme	stringent	1762:1770	arg1	testing					1777:1783	stringent data testing	1762:1783	stringent data testing	1762:1783	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	4	41	theme	inspection	591:600	arg1	importance					568:577	the importance	564:577	the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization	564:723	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
31111416	1	42	gly	glycoprotein	125:136	arg1	glycoprotein					125:136	HIV Env glycoprotein	117:136	HIV Env glycoprotein	117:136	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	11	43	theme	data	1772:1775	arg1	testing					1777:1783	stringent data testing	1762:1783	stringent data testing	1762:1783	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	1	44	theme	Env	121:123	arg1	glycoprotein					125:136	HIV Env glycoprotein	117:136	HIV Env glycoprotein	117:136	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	45	theme	programs	222:229	arg1	step					183:186	the essential step	169:186	the essential step of structure-based vaccine design programs	169:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	45	theme	programs	222:229	arg1	Characterization					97:112	Characterization	97:112	Characterization of HIV Env glycoprotein with 28 glycosylation sites	97:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	7	46	theme	structural	1134:1143	arg1	characterization					1145:1160	full structural characterization	1129:1160	full structural characterization of this molecule	1129:1177	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	4	47	theme	thorough	582:589	arg1	inspection					591:600	thorough inspection	582:600	thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization	582:723	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
31111416	1	48	gly	glycosylation	146:158	arg2	sites					160:164	28 glycosylation sites	143:164	28 glycosylation sites	143:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	48	gly	glycosylation	146:158	arg2	28					143:144	28	143:144	28	143:144	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	5	49	theme	automatic	869:877	arg1	analysis					884:891	the automatic data analysis	865:891	the automatic data analysis	865:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	2	50	theme	mapping	265:271	arg1	analysis					273:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	4	51	theme	biopharmaceutical	690:706	arg1	characterization					708:723	the biopharmaceutical characterization	686:723	the biopharmaceutical characterization	686:723	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
31111416	2	52	theme	immunogen	428:436	arg1	adequate					395:402	adequate	395:402	adequate	395:402	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	7	53	theme	characterization	1145:1160	arg1	claim					1120:1124	any claim	1116:1124	any claim of full structural characterization of this molecule beyond these limits	1116:1197	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	11	54	theme	glycan	1722:1727	arg1	characterization					1729:1744	glycan characterization	1722:1744	glycan characterization	1722:1744	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	8	55	theme	glycan	1278:1283	arg1	occupancy					1285:1293	the percent glycan occupancy	1266:1293	the percent glycan occupancy	1266:1293	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	0	56	theme	Structural	25:34	arg1	Characterization					36:51	Structural Characterization	25:51	Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS	25:94	Overcoming Challenges in Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS.					
31111416	2	57	theme	native	421:426	arg1	immunogen					428:436	the native immunogen	417:436	the native immunogen	417:436	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	0	58	theme	Envelope	62:69	arg1	Glycoprotein					71:82	HIV-1 Envelope Glycoprotein	56:82	HIV-1 Envelope Glycoprotein	56:82	Overcoming Challenges in Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS.					
31111416	10	59	theme	expected	1641:1648	arg1	patch					1659:1663	the expected "mannose patch	1637:1663	the expected "mannose patch	1637:1663	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	2	60	gly	occupancy	364:372	arg2	Env					377:379	Env	377:379	Env	377:379	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	5	61	theme	occasional	791:800	arg1	gaps					814:817	occasional information gaps	791:817	occasional information gaps that could not possibly be filled by means of the automatic data analysis	791:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	2	62	theme	LC-MS/MS	248:255	arg1	analysis					273:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	1	63	theme	glycoprotein	125:136	arg1	step					183:186	the essential step	169:186	the essential step of structure-based vaccine design programs	169:229	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	1	63	theme	glycoprotein	125:136	arg1	Characterization					97:112	Characterization	97:112	Characterization of HIV Env glycoprotein with 28 glycosylation sites	97:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	0	64	theme	HIV-1	56:60	arg1	Glycoprotein					71:82	HIV-1 Envelope Glycoprotein	56:82	HIV-1 Envelope Glycoprotein	56:82	Overcoming Challenges in Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS.					
31111416	11	65	from	focus	1753:1757	arg1	testing					1777:1783	stringent data testing	1762:1783	stringent data testing	1762:1783	Experimental procedure optimization and a workflow for glycan characterization with a focus on stringent data testing are presented in the current study.					
31111416	2	66	theme	comprehensive	234:246	arg1	analysis					273:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	9	67	theme	exact	1384:1388	arg1	profiles					1397:1404	The exact glycan profiles	1380:1404	The exact glycan profiles	1380:1404	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	7	68	theme	full	1129:1132	arg1	characterization					1145:1160	full structural characterization	1129:1160	full structural characterization of this molecule	1129:1177	It was shown that the glycan sites can only be characterized to a certain limit, and that any claim of full structural characterization of this molecule beyond these limits should be treated with caution.					
31111416	2	69	theme	peptide	257:263	arg1	analysis					273:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis	232:280	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	2	70	theme	glycosite	354:362	arg1	occupancy					364:372	glycosite occupancy	354:372	glycosite occupancy of Env	354:379	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	1	71	theme	HIV	117:119	arg1	glycoprotein					125:136	HIV Env glycoprotein	117:136	HIV Env glycoprotein	117:136	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	8	72	theme	percent	1270:1276	arg1	occupancy					1285:1293	the percent glycan occupancy	1266:1293	the percent glycan occupancy	1266:1293	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	2	73	gly	glycosite	354:362	arg2	glycosite					354:362	glycosite occupancy	354:372	glycosite occupancy of Env	354:379	A comprehensive LC-MS/MS peptide mapping analysis was applied to assess the primary sequence, glycosylation profiles, and glycosite occupancy of Env to ensure the adequate mimicking of the native immunogen.					
31111416	5	74	with	data	781:784	arg1	gaps					814:817	occasional information gaps	791:817	occasional information gaps that could not possibly be filled by means of the automatic data analysis	791:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	1	75	theme	glycosylation	146:158	arg1	sites					160:164	28 glycosylation sites	143:164	28 glycosylation sites	143:164	Characterization of HIV Env glycoprotein with 28 glycosylation sites is the essential step of structure-based vaccine design programs.					
31111416	3	76	theme	cleaved	495:501	arg1	subunits					503:510	its cleaved subunits	491:510	its cleaved subunits within the trimeric assembly	491:539	Another structural feature was reported, related to its cleaved subunits within the trimeric assembly.					
31111416	5	77	theme	data	879:882	arg1	analysis					884:891	the automatic data analysis	865:891	the automatic data analysis	865:891	The complexity of Env translates into a vast amount of data with occasional information gaps that could not possibly be filled by means of the automatic data analysis.					
31111416	9	78	theme	glycosylation	1462:1474	arg1	type					1476:1479	only the glycosylation type	1453:1479	only the glycosylation type	1453:1479	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	8	79	theme	N-glycan	1315:1322	arg1	sites					1324:1328	25 N-glycan sites	1312:1328	25 N-glycan sites	1312:1328	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	8	79	theme	N-glycan	1315:1322	arg1	sites					1373:1377	3 critical antibody-recognition sites	1341:1377	3 critical antibody-recognition sites	1341:1377	Following the result verification, the percent glycan occupancy was reported for 25 N-glycan sites, including 3 critical antibody-recognition sites.					
31111416	0	80	theme	Glycoprotein	71:82	arg1	Characterization					36:51	Structural Characterization	25:51	Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS	25:94	Overcoming Challenges in Structural Characterization of HIV-1 Envelope Glycoprotein by LC-MS/MS.					
31111416	10	81	theme	mannose-type	1600:1611	arg1	glycans					1613:1619	the unprocessed high mannose-type glycans	1579:1619	the unprocessed high mannose-type glycans	1579:1619	The distribution of the unprocessed high mannose-type glycans correlated with the expected "mannose patch."					
31111416	9	82	theme	glycan	1390:1395	arg1	profiles					1397:1404	The exact glycan profiles	1380:1404	The exact glycan profiles	1380:1404	The exact glycan profiles were provided for 20 individual sites, whereas only the glycosylation type could be deduced for 5 sites, dictated by their location within Env sequence.					
31111416	4	83	theme	informatics	634:644	arg1	available					672:680	available	672:680	available	672:680	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
31111416	4	83	theme	informatics	634:644	arg1	tools					646:650	the informatics tools	630:650	the informatics tools which are currently available for the biopharmaceutical characterization	630:723	We bring attention to the importance of thorough inspection of the results generated by the informatics tools which are currently available for the biopharmaceutical characterization.					
30578591	0	0	theme	Host	91:94	arg1	Effects					80:86	the Effects	76:86	the Effects of Host and Glycosite Location on Site-Specific Glycan Structures	76:152	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	4	1	theme	glycosylation	676:688	arg1	site					690:693	one partial glycosylation site	664:693	one partial glycosylation site from both cell lines	664:714	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	2	gly	glycosylated	640:651	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	2	gly	glycosylated	640:651	arg1	three					583:587	three	583:587	three	583:587	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	2	3	theme	glycosylation	354:366	arg1	information					368:378	detailed site-specific glycosylation information	331:378	detailed site-specific glycosylation information of NA	331:384	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	5	4	contain	has	749:751	arg1	NA					721:722	The NA	717:722	The NA expressed in human cells	717:747	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	5	4	contain	has	749:751	arg2	glycans					766:772	more complex glycans	753:772	more complex glycans	753:772	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	5	5	theme	complex	758:764	arg1	glycans					766:772	more complex glycans	753:772	more complex glycans	753:772	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	2	6	theme	site-specific	340:352	arg1	information					368:378	detailed site-specific glycosylation information	331:378	detailed site-specific glycosylation information of NA	331:384	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	6	7	theme	NA	992:993	arg1	NA					992:993	NA	992:993	NA	992:993	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	6	7	theme	NA	992:993	arg1	glycosites					978:987	different glycosites	968:987	different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA	968:1076	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	0	8	theme	Glycosite	100:108	arg1	Location					110:117	Glycosite Location	100:117	Glycosite Location	100:117	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	7	9	theme	valuable	1099:1106	arg1	information					1108:1118	valuable information	1099:1118	valuable information for the research of influenza virus as well as the functions of viral protein glycosylation	1099:1210	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	7	10	theme	glycosylation	1198:1210	arg1	information					1108:1118	valuable information	1099:1118	valuable information for the research of influenza virus as well as the functions of viral protein glycosylation	1099:1210	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	7	10	theme	glycosylation	1198:1210	arg1	functions					1171:1179	the functions	1167:1179	valuable information for the research of influenza virus as well as the functions of viral protein glycosylation	1099:1210	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	3	11	theme	Hi-5	548:551	arg1	cells					553:557	human 293T and insect Hi-5 cells	526:557	cells	553:557	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	7	12	theme	protein	1190:1196	arg1	glycosylation					1198:1210	viral protein glycosylation	1184:1210	viral protein glycosylation	1184:1210	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	7	13	theme	influenza	1140:1148	arg1	virus					1150:1154	influenza virus	1140:1154	influenza virus	1140:1154	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	4	14	theme	cell	705:708	arg1	lines					710:714	both cell lines	700:714	both cell lines	700:714	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	15	theme	N-linked	607:614	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	5	16	theme	appropriate	843:853	arg1	system					866:871	an appropriate expression system	840:871	an appropriate expression system for the production of functional glycoproteins	840:918	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	3	17	theme	glycopeptide	426:437	arg1	analysis					439:446	intact glycopeptide analysis	419:446	intact glycopeptide analysis	419:446	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	0	18	theme	Location	110:117	arg1	Effects					80:86	the Effects	76:86	the Effects of Host and Glycosite Location on Site-Specific Glycan Structures	76:152	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	4	19	gly	glycosylation	616:628	arg2	four					592:595	four	592:595	four	592:595	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	19	gly	glycosylation	616:628	arg2	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	3	20	theme	human	526:530	arg1	293T					532:535	human 293T and insect Hi-5 cells	526:557	293T	532:535	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	0	21	theme	Glycopeptide	7:18	arg1	Analysis					20:27	Intact Glycopeptide Analysis	0:27	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase	0:64	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	2	22	theme	influenza	310:318	arg1	virus					320:324	the influenza virus	306:324	the influenza virus	306:324	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	6	23	theme	glycosylation	1058:1070	arg1	site-dependence					1039:1053	the site-dependence	1035:1053	the site-dependence of glycosylation on NA	1035:1076	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	1	24	theme	serious	188:194	arg1	threat					196:201	a serious threat	186:201	a serious threat to human health	186:217	Influenza H1N1 virus has posed a serious threat to human health.					
30578591	0	25	theme	Intact	0:5	arg1	Analysis					20:27	Intact Glycopeptide Analysis	0:27	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase	0:64	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	6	26	theme	different	968:976	arg1	NA					992:993	NA	992:993	NA	992:993	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	6	26	theme	different	968:976	arg1	glycosites					978:987	different glycosites	968:987	different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA	968:1076	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	6	27	from	site-dependence	1039:1053	arg1	NA					1075:1076	NA	1075:1076	NA	1075:1076	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	0	28	theme	Neuraminidase	52:64	arg1	Analysis					20:27	Intact Glycopeptide Analysis	0:27	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase	0:64	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	2	29	theme	NA	383:384	arg1	information					368:378	detailed site-specific glycosylation information	331:378	detailed site-specific glycosylation information of NA	331:384	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	5	30	theme	insect	787:792	arg1	cells					794:798	insect cells	787:798	insect cells	787:798	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	0	31	theme	Glycan	136:141	arg1	Structures					143:152	Site-Specific Glycan Structures	122:152	Site-Specific Glycan Structures	122:152	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	3	32	theme	insect	541:546	arg1	cells					553:557	human 293T and insect Hi-5 cells	526:557	cells	553:557	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	4	33	theme	glycosylation	616:628	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	0	34	theme	Site-Specific	122:134	arg1	Structures					143:152	Site-Specific Glycan Structures	122:152	Site-Specific Glycan Structures	122:152	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	2	35	dep	infectivity	277:287	arg1	the					273:275	the	273:275	the	273:275	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	7	36	theme	virus	1150:1154	arg1	research					1128:1135	the research	1124:1135	the research of influenza virus	1124:1154	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	1	37	theme	human	206:210	arg1	health					212:217	human health	206:217	human health	206:217	Influenza H1N1 virus has posed a serious threat to human health.					
30578591	0	38	theme	A/H1N1/09	42:50	arg1	Neuraminidase					52:64	Influenza A/H1N1/09 Neuraminidase	32:64	Influenza A/H1N1/09 Neuraminidase	32:64	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	2	39	theme	neuraminidase	241:253	arg1	glycosylation					224:236	The glycosylation	220:236	The glycosylation of neuraminidase (NA)	220:258	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	3	40	theme	intact	419:424	arg1	analysis					439:446	intact glycopeptide analysis	419:446	intact glycopeptide analysis	419:446	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	0	41	theme	Influenza	32:40	arg1	Neuraminidase					52:64	Influenza A/H1N1/09 Neuraminidase	32:64	Influenza A/H1N1/09 Neuraminidase	32:64	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	6	42	theme	glycans	940:946	arg1	types					931:935	Different types	921:935	Different types of glycans	921:946	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	5	43	gly	glycoproteins	906:918	arg1	glycoproteins					906:918	functional glycoproteins	895:918	functional glycoproteins	895:918	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	0	44	from	Effects	80:86	arg1	Structures					143:152	Site-Specific Glycan Structures	122:152	Site-Specific Glycan Structures	122:152	Intact Glycopeptide Analysis of Influenza A/H1N1/09 Neuraminidase Revealing the Effects of Host and Glycosite Location on Site-Specific Glycan Structures.					
30578591	6	45	theme	human	1008:1012	arg1	cells					1014:1018	human cells	1008:1018	human cells	1008:1018	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	4	46	theme	sites	630:634	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	46	theme	sites	630:634	arg1	three					583:587	three	583:587	three	583:587	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	7	47	theme	viral	1184:1188	arg1	glycosylation					1198:1210	viral protein glycosylation	1184:1210	viral protein glycosylation	1184:1210	This study provides valuable information for the research of influenza virus as well as the functions of viral protein glycosylation.					
30578591	2	48	theme	detailed	331:338	arg1	information					368:378	detailed site-specific glycosylation information	331:378	detailed site-specific glycosylation information of NA	331:384	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	2	49	gly	glycosylation	224:236	arg1	NA					256:257	NA	256:257	NA	256:257	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	2	49	gly	glycosylation	224:236	arg1	virus					320:324	the influenza virus	306:324	the influenza virus	306:324	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	2	49	gly	glycosylation	224:236	arg1	neuraminidase					241:253	neuraminidase	241:253	neuraminidase (NA)	241:258	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	4	50	gly	glycosylation	676:688	arg2	one					664:666	one	664:666	one	664:666	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	50	gly	glycosylation	676:688	arg2	site					690:693	one partial glycosylation site	664:693	one partial glycosylation site from both cell lines	664:714	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	6	51	gly	glycosites	978:987	arg1	NA					992:993	NA	992:993	NA	992:993	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	6	51	gly	glycosites	978:987	arg2	glycosites					978:987	different glycosites	968:987	different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA	968:1076	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	6	51	gly	glycosites	978:987	arg2	NA					992:993	NA	992:993	NA	992:993	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	5	52	theme	functional	895:904	arg1	glycoproteins					906:918	functional glycoproteins	895:918	functional glycoproteins	895:918	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	6	53	theme	Different	921:929	arg1	types					931:935	Different types	921:935	Different types of glycans	921:946	Different types of glycans are identified from different glycosites of NA expressed in human cells, which implies the site-dependence of glycosylation on NA.					
30578591	5	54	theme	expression	855:864	arg1	system					866:871	an appropriate expression system	840:871	an appropriate expression system for the production of functional glycoproteins	840:918	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	4	55	theme	partial	668:674	arg1	site					690:693	one partial glycosylation site	664:693	one partial glycosylation site from both cell lines	664:714	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	3	56	theme	influenza	467:475	arg1	A/H1N1/California/2009					481:502	A/H1N1/California/2009	481:502	A/H1N1/California/2009	481:502	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	3	56	theme	influenza	467:475	arg1	NA					477:478	an influenza NA	464:478	an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells	464:557	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	4	57	from	lines	710:714	arg1	site					690:693	one partial glycosylation site	664:693	one partial glycosylation site from both cell lines	664:714	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	3	58	gly	glycopeptide	426:437	arg2	glycopeptide					426:437	intact glycopeptide analysis	419:446	intact glycopeptide analysis	419:446	In this study, intact glycopeptide analysis is performed on an influenza NA (A/H1N1/California/2009) that is expressed in human 293T and insect Hi-5 cells.					
30578591	2	59	theme	virus	320:324	arg1	infectivity					277:287	infectivity	277:287	infectivity	277:287	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	2	59	theme	virus	320:324	arg1	virulence					293:301	virulence	293:301	virulence	293:301	The glycosylation of neuraminidase (NA) could affect the infectivity and virulence of the influenza virus, but detailed site-specific glycosylation information of NA is still missing.					
30578591	5	60	theme	glycoproteins	906:918	arg1	production					881:890	the production	877:890	the production of functional glycoproteins	877:918	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	5	61	theme	human	737:741	arg1	cells					743:747	human cells	737:747	human cells	737:747	The NA expressed in human cells has more complex glycans than that of insect cells, suggesting the importance of selecting an appropriate expression system for the production of functional glycoproteins.					
30578591	1	62	theme	Influenza	155:163	arg1	virus					170:174	Influenza H1N1 virus	155:174	Influenza H1N1 virus	155:174	Influenza H1N1 virus has posed a serious threat to human health.					
30578591	4	63	theme	potential	597:605	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	4	64	link	N-linked	607:614	arg1	sites					630:634	four potential N-linked glycosylation sites	592:634	four potential N-linked glycosylation sites	592:634	The data indicate that three of four potential N-linked glycosylation sites are glycosylated, including one partial glycosylation site from both cell lines.					
30578591	1	65	theme	H1N1	165:168	arg1	virus					170:174	Influenza H1N1 virus	155:174	Influenza H1N1 virus	155:174	Influenza H1N1 virus has posed a serious threat to human health.					
31227230	0	0	theme	Glycome	70:76	arg1	Display					49:55	Display	49:55	Display of the Human Glycome by Gene Engineered Cells	49:101	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	3	1	theme	limited	306:312	arg1	arrays					295:300	Glycan arrays	288:300	Glycan arrays	288:300	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	3	1	theme	limited	306:312	arg1	resources					314:322	limited resources	306:322	limited resources	306:322	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	4	2	theme	biosynthetic	594:605	arg1	basis					623:627	the genetic, biosynthetic, and structural basis	581:627	the genetic, biosynthetic, and structural basis for glycan binding	581:646	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	0	3	theme	Human	64:68	arg1	Glycome					70:76	the Human Glycome	60:76	the Human Glycome	60:76	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	4	4	theme	glycosylation	524:536	arg1	capacities					538:547	combinatorially engineered glycosylation capacities	497:547	combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context	497:667	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	6	5	theme	O-glycans	1144:1152	arg1	patches					1133:1139	clustered patches	1123:1139	clustered patches of O-glycans organized by their presentation on proteins	1123:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	1	6	theme	cells-the	143:151	arg1	glycome-is					153:162	cells-the glycome-is vast	143:167	cells-the glycome-is vast	143:167	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	4	7	theme	engineered	513:522	arg1	capacities					538:547	combinatorially engineered glycosylation capacities	497:547	combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context	497:667	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	5	8	dep	synthesis	930:938	arg1	strategies					988:997	strategies	988:997	strategies	988:997	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	6	9	theme	array	1039:1043	arg1	utility					1006:1012	Broad utility	1000:1012	Broad utility of the cell-based glycan array	1000:1043	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	4	10	theme	isogenic	470:477	arg1	cells					486:490	isogenic HEK293 cells	470:490	isogenic HEK293 cells	470:490	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	0	11	dep	Engineered	86:95	arg1	Gene					81:84	Gene	81:84	Gene	81:84	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	2	12	theme	binding	270:276	arg1	epitopes					278:285	glycan hapten binding epitopes	256:285	glycan hapten binding epitopes	256:285	Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes.					
31227230	6	13	theme	glycan	1032:1037	arg1	array					1039:1043	the cell-based glycan array	1017:1043	the cell-based glycan array	1017:1043	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	1	14	from	glycans	132:138	arg1	glycome-is					153:162	cells-the glycome-is vast	143:167	cells-the glycome-is vast	143:167	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	1	14	from	glycans	132:138	arg1	complex					173:179	complex	173:179	complex	173:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	2	15	theme	hapten	263:268	arg1	epitopes					278:285	glycan hapten binding epitopes	256:285	glycan hapten binding epitopes	256:285	Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes.					
31227230	2	16	used	used	241:244	arg2	arrays					201:206	Glycan arrays	194:206	Glycan arrays	194:206	Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes.					
31227230	4	17	with	library	459:465	arg1	capacities					538:547	combinatorially engineered glycosylation capacities	497:547	combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context	497:667	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	0	18	theme	Engineered	86:95	arg1	Cells					97:101	Gene Engineered Cells	81:101	Gene Engineered Cells	81:101	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	4	19	theme	cells	486:490	arg1	library					459:465	a library	457:465	a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context	457:667	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	5	20	theme	logical	802:808	arg1	steps					834:838	logical sequential biosynthetic steps	802:838	logical sequential biosynthetic steps	802:838	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	2	21	theme	glycan	256:261	arg1	epitopes					278:285	glycan hapten binding epitopes	256:285	glycan hapten binding epitopes	256:285	Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes.					
31227230	1	22	theme	vast	164:167	arg1	glycome-is					153:162	cells-the glycome-is vast	143:167	cells-the glycome-is vast	143:167	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	3	23	theme	glycans	377:383	arg1	context					360:366	the context	356:366	the context of other glycans and glycoconjugates	356:403	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	4	24	theme	HEK293	479:484	arg1	cells					486:490	isogenic HEK293 cells	470:490	isogenic HEK293 cells	470:490	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	6	25	theme	clustered	1123:1131	arg1	patches					1133:1139	clustered patches	1123:1139	clustered patches of O-glycans organized by their presentation on proteins	1123:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	1	26	from	diversity	119:127	arg1	glycome-is					153:162	cells-the glycome-is vast	143:167	cells-the glycome-is vast	143:167	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	1	26	from	diversity	119:127	arg1	complex					173:179	complex	173:179	complex	173:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	6	27	theme	adhesins	1111:1118	arg1	binding					1090:1096	higher order binding	1077:1096	higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins	1077:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	6	28	theme	cell-based	1021:1030	arg1	array					1039:1043	the cell-based glycan array	1017:1043	the cell-based glycan array	1017:1043	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	5	29	theme	cell-based	674:683	arg1	self-renewable					701:714	self-renewable	701:714	self-renewable	701:714	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	29	theme	cell-based	674:683	arg1	array					692:696	The cell-based glycan array	670:696	The cell-based glycan array	670:696	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	0	30	theme	Human	12:16	arg1	Pathways					32:39	Human Glycosylation Pathways	12:39	Human Glycosylation Pathways	12:39	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	5	31	theme	biosynthetic	821:832	arg1	steps					834:838	logical sequential biosynthetic steps	802:838	logical sequential biosynthetic steps	802:838	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	3	32	theme	other	371:375	arg1	glycans					377:383	other glycans	371:383	other glycans	371:383	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	4	33	theme	genetic	585:591	arg1	basis					623:627	the genetic, biosynthetic, and structural basis	581:627	the genetic, biosynthetic, and structural basis for glycan binding	581:646	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	5	34	theme	recombinant	941:951	arg1	production					953:962	recombinant production	941:962	recombinant production	941:962	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	35	theme	structural	864:873	arg1	features					882:889	structural glycan features	864:889	structural glycan features involved	864:898	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	0	36	theme	Pathways	32:39	arg1	Atlas					3:7	An Atlas	0:7	An Atlas of Human Glycosylation Pathways	0:39	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	5	37	theme	glycosyltransferase	728:746	arg1	blocking					767:774	blocking	767:774	blocking	767:774	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	37	theme	glycosyltransferase	728:746	arg1	genes					748:752	glycosyltransferase genes	728:752	glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies	728:997	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	38	theme	glycan	875:880	arg1	features					882:889	structural glycan features	864:889	structural glycan features involved	864:898	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	6	39	theme	microbial	1101:1109	arg1	adhesins					1111:1118	microbial adhesins	1101:1118	microbial adhesins	1101:1118	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	4	40	theme	natural	653:659	arg1	context					661:667	a natural context	651:667	a natural context	651:667	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	0	41	theme	Glycosylation	18:30	arg1	Pathways					32:39	Human Glycosylation Pathways	12:39	Human Glycosylation Pathways	12:39	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.					
31227230	6	42	theme	Broad	1000:1004	arg1	utility					1006:1012	Broad utility	1000:1012	Broad utility of the cell-based glycan array	1000:1043	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	5	43	theme	sequential	810:819	arg1	steps					834:838	logical sequential biosynthetic steps	802:838	logical sequential biosynthetic steps	802:838	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	44	theme	features	882:889	arg1	predictive					850:859	predictive	850:859	predictive	850:859	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	3	45	theme	glycoconjugates	389:403	arg1	context					360:366	the context	356:366	the context of other glycans and glycoconjugates	356:403	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	5	46	theme	glycan	685:690	arg1	self-renewable					701:714	self-renewable	701:714	self-renewable	701:714	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	46	theme	glycan	685:690	arg1	array					692:696	The cell-based glycan array	670:696	The cell-based glycan array	670:696	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	2	47	theme	Glycan	194:199	arg1	arrays					201:206	Glycan arrays	194:206	Glycan arrays	194:206	Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes.					
31227230	6	48	theme	higher	1077:1082	arg1	binding					1090:1096	higher order binding	1077:1096	higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins	1077:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	4	49	used	used	409:412	arg2	We					406:407	We	406:407	We	406:407	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	5	50	dep	required	754:761	arg1	blocking					767:774	blocking	767:774	blocking	767:774	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	5	50	dep	required	754:761	arg1	genes					748:752	glycosyltransferase genes	728:752	glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies	728:997	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	6	51	from	presentation	1173:1184	arg1	proteins					1189:1196	proteins	1189:1196	proteins	1189:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
31227230	1	52	theme	structural	108:117	arg1	diversity					119:127	The structural diversity	104:127	The structural diversity of glycans on cells-the glycome-is vast and complex	104:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	3	53	theme	Glycan	288:293	arg1	arrays					295:300	Glycan arrays	288:300	Glycan arrays	288:300	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	3	53	theme	Glycan	288:293	arg1	resources					314:322	limited resources	306:322	limited resources	306:322	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	4	54	theme	pathways	436:443	arg1	maps					414:417	maps	414:417	maps of glycosylation pathways	414:443	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	5	55	theme	genetic	969:975	arg1	dissection					977:986	genetic dissection	969:986	genetic dissection	969:986	The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies.					
31227230	4	56	theme	structural	612:621	arg1	basis					623:627	the genetic, biosynthetic, and structural basis	581:627	the genetic, biosynthetic, and structural basis for glycan binding	581:646	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	4	57	theme	glycosylation	422:434	arg1	pathways					436:443	glycosylation pathways	422:443	glycosylation pathways	422:443	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	1	58	from	complex	173:179	arg1	diversity					119:127	The structural diversity	104:127	The structural diversity of glycans on cells-the glycome-is vast and complex	104:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	4	59	theme	glycan	633:638	arg1	binding					640:646	glycan binding	633:646	glycan binding	633:646	We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context.					
31227230	3	60	theme	present	328:334	arg1	saccharides					336:346	present saccharides	328:346	present saccharides	328:346	Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates.					
31227230	1	61	from	glycome-is	153:162	arg1	diversity					119:127	The structural diversity	104:127	The structural diversity of glycans on cells-the glycome-is vast and complex	104:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	1	62	theme	glycans	132:138	arg1	diversity					119:127	The structural diversity	104:127	The structural diversity of glycans on cells-the glycome-is vast and complex	104:179	The structural diversity of glycans on cells-the glycome-is vast and complex to decipher.					
31227230	6	63	theme	order	1084:1088	arg1	binding					1090:1096	higher order binding	1077:1096	higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins	1077:1196	Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.					
30280442	0	0	theme	Glycans	81:87	arg1	Core					48:51	the Highly Branched Hexasaccharide Core	13:51	the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans	13:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	5	1	theme	target	769:774	arg1	molecule					776:783	The target molecule	765:783	The target molecule	765:783	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	0	2	theme	N-Linked	72:79	arg1	Glycans					81:87	Chlorella Virus N-Linked Glycans	56:87	Chlorella Virus N-Linked Glycans	56:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	6	3	theme	linear	935:940	arg1	approaches					942:951	Both convergent and linear approaches	915:951	Both convergent and linear approaches	915:951	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	4	4	attach	present	710:716	arg1	structures					736:745	the >15 structures	728:745	the >15 structures reported to date	728:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	4	attach	present	710:716	arg2	pentasaccharide					694:708	the pentasaccharide	690:708	the pentasaccharide present in all of the >15 structures reported to date	690:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	4	attach	present	710:716	arg1	all					721:723	all	721:723	all	721:723	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	5	from	all	721:723	arg1	present					710:716	present	710:716	present	710:716	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	1	6	link	N-linked	206:213	arg1	glycans					215:221	all other N-linked glycans	196:221	all other N-linked glycans	196:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	4	7	theme	virus	620:624	arg1	hexasaccharide					661:674	a highly branched hexasaccharide	643:674	a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	643:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	7	theme	virus	620:624	arg1	glycan					635:640	a chlorella virus N-linked glycan	608:640	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	6	8	theme	convergent	920:929	arg1	approaches					942:951	Both convergent and linear approaches	915:951	Both convergent and linear approaches	915:951	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	4	9	from	present	710:716	arg1	structures					736:745	the >15 structures	728:745	the >15 structures reported to date	728:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	9	from	present	710:716	arg1	all					721:723	all	721:723	all	721:723	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	2	10	theme	host	322:325	arg1	machinery					305:313	the biosynthetic machinery	288:313	the biosynthetic machinery of the host to make glycocoproteins	288:349	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	5	11	theme	glucosyl-asparagine	796:814	arg1	linkage					816:822	a glucosyl-asparagine linkage	794:822	a glucosyl-asparagine linkage	794:822	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	3	12	theme	structurally-unprecedented	494:519	arg1	glycans					521:527	these fascinating and structurally-unprecedented glycans	472:527	these fascinating and structurally-unprecedented glycans	472:527	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	6	13	from	target	1021:1026	arg1	yield					1057:1061	13 % overall yield	1044:1061	13 % overall yield	1044:1061	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	6	13	from	target	1021:1026	arg1	steps					1034:1038	16 steps	1031:1038	16 steps	1031:1038	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	6	14	theme	%	1047:1047	arg1	yield					1057:1061	13 % overall yield	1044:1061	13 % overall yield	1044:1061	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	2	15	theme	carbohydrate-processing	384:406	arg1	enzymes					408:414	their own carbohydrate-processing enzymes	374:414	their own carbohydrate-processing enzymes	374:414	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	4	16	theme	chlorella	610:618	arg1	virus					620:624	a chlorella virus	608:624	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	17	theme	N-linked	626:633	arg1	hexasaccharide					661:674	a highly branched hexasaccharide	643:674	a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	643:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	17	theme	N-linked	626:633	arg1	glycan					635:640	a chlorella virus N-linked glycan	608:640	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	2	18	theme	own	380:382	arg1	enzymes					408:414	their own carbohydrate-processing enzymes	374:414	their own carbohydrate-processing enzymes	374:414	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	5	19	theme	hyperbranched	831:843	arg1	residue					853:859	a "hyperbranched" fucose residue	828:859	a "hyperbranched" fucose residue	828:859	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	4	20	theme	first	589:593	arg1	synthesis					595:603	the first synthesis	585:603	the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	585:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	21	theme	present	710:716	arg1	pentasaccharide					694:708	the pentasaccharide	690:708	the pentasaccharide present in all of the >15 structures reported to date	690:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	5	22	theme	"	844:844	arg1	residue					853:859	a "hyperbranched" fucose residue	828:859	a "hyperbranched" fucose residue	828:859	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	1	23	with	glycoproteins	125:137	arg1	moieties					157:164	carbohydrate moieties	144:164	carbohydrate moieties	144:164	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	1	24	theme	Chlorella	90:98	arg1	viruses					100:106	Chlorella viruses	90:106	Chlorella viruses	90:106	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	1	25	theme	other	200:204	arg1	glycans					215:221	all other N-linked glycans	196:221	all other N-linked glycans	196:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	5	26	theme	fucose	846:851	arg1	residue					853:859	a "hyperbranched" fucose residue	828:859	a "hyperbranched" fucose residue	828:859	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	1	27	gly	glycoproteins	125:137	arg1	glycoproteins					125:137	N-linked glycoproteins	116:137	N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans	116:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	1	28	theme	N-linked	206:213	arg1	glycans					215:221	all other N-linked glycans	196:221	all other N-linked glycans	196:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	2	29	theme	biosynthetic	292:303	arg1	machinery					305:313	the biosynthetic machinery	288:313	the biosynthetic machinery of the host to make glycocoproteins	288:349	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	5	30	theme	hydroxyl	881:888	arg1	groups					890:895	the hydroxyl groups	877:895	the hydroxyl groups	877:895	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	0	31	theme	Hexasaccharide	33:46	arg1	Core					48:51	the Highly Branched Hexasaccharide Core	13:51	the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans	13:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	4	32	theme	>15	732:734	arg1	structures					736:745	the >15 structures	728:745	the >15 structures reported to date	728:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	2	33	theme	most	244:247	arg1	viruses					249:255	most viruses	244:255	most viruses	244:255	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	0	34	theme	Branched	24:31	arg1	Core					48:51	the Highly Branched Hexasaccharide Core	13:51	the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans	13:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	3	35	theme	better	419:424	arg1	understanding					426:438	A better understanding	417:438	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans	417:527	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	3	36	theme	assembly	460:467	arg1	understanding					426:438	A better understanding	417:438	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans	417:527	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	4	37	link	N-linked	626:633	arg1	hexasaccharide					661:674	a highly branched hexasaccharide	643:674	a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	643:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	37	link	N-linked	626:633	arg1	glycan					635:640	a chlorella virus N-linked glycan	608:640	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	1	38	theme	N-linked	116:123	arg1	glycoproteins					125:137	N-linked glycoproteins	116:137	N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans	116:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	0	39	theme	Core	48:51	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.	0:88	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	5	40	gly	glycosylated	901:912	arg1	all					870:872	all	870:872	all	870:872	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	5	40	gly	glycosylated	901:912	arg2	groups					890:895	the hydroxyl groups	877:895	the hydroxyl groups	877:895	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	5	40	gly	glycosylated	901:912	arg1	residue					853:859	a "hyperbranched" fucose residue	828:859	a "hyperbranched" fucose residue	828:859	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	5	40	gly	glycosylated	901:912	arg1	linkage					816:822	a glucosyl-asparagine linkage	794:822	a glucosyl-asparagine linkage	794:822	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	5	40	gly	glycosylated	901:912	arg1	groups					890:895	the hydroxyl groups	877:895	the hydroxyl groups	877:895	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	5	40	gly	glycosylated	901:912	arg2	all					870:872	all	870:872	all	870:872	The target molecule includes a glucosyl-asparagine linkage and a "hyperbranched" fucose residue in which all of the hydroxyl groups are glycosylated.					
30280442	3	41	dep	function	447:454	arg1	the					443:445	the	443:445	the	443:445	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	3	42	theme	glycans	521:527	arg1	assembly					460:467	assembly	460:467	assembly	460:467	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	3	42	theme	glycans	521:527	arg1	function					447:454	function	447:454	function	447:454	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	1	43	link	N-linked	116:123	arg1	glycoproteins					125:137	N-linked glycoproteins	116:137	N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans	116:221	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	6	44	theme	13 	1044:1046	arg1	%					1047:1047	%	1047:1047	%	1047:1047	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	3	45	theme	fascinating	478:488	arg1	glycans					521:527	these fascinating and structurally-unprecedented glycans	472:527	these fascinating and structurally-unprecedented glycans	472:527	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	4	46	theme	glycan	635:640	arg1	synthesis					595:603	the first synthesis	585:603	the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	585:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	47	contain	contains	681:688	arg1	hexasaccharide					661:674	a highly branched hexasaccharide	643:674	a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	643:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	47	contain	contains	681:688	arg1	glycan					635:640	a chlorella virus N-linked glycan	608:640	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	47	contain	contains	681:688	arg2	pentasaccharide					694:708	the pentasaccharide	690:708	the pentasaccharide present in all of the >15 structures reported to date	690:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	1	48	theme	carbohydrate	144:155	arg1	moieties					157:164	carbohydrate moieties	144:164	carbohydrate moieties	144:164	Chlorella viruses produce N-linked glycoproteins with carbohydrate moieties that differ in structure from all other N-linked glycans.					
30280442	3	49	theme	function	447:454	arg1	understanding					426:438	A better understanding	417:438	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans	417:527	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
30280442	0	50	theme	Virus	66:70	arg1	Glycans					81:87	Chlorella Virus N-Linked Glycans	56:87	Chlorella Virus N-Linked Glycans	56:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	6	51	theme	overall	1049:1055	arg1	yield					1057:1061	13 % overall yield	1044:1061	13 % overall yield	1044:1061	Both convergent and linear approaches were investigated with the latter being successful in providing the target in 16 steps and 13 % overall yield.					
30280442	4	52	theme	branched	652:659	arg1	hexasaccharide					661:674	a highly branched hexasaccharide	643:674	a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date	643:762	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	4	52	theme	branched	652:659	arg1	glycan					635:640	a chlorella virus N-linked glycan	608:640	a chlorella virus N-linked glycan	608:640	This work describes the first synthesis of a chlorella virus N-linked glycan, a highly branched hexasaccharide that contains the pentasaccharide present in all of the >15 structures reported to date.					
30280442	2	53	from	hijack	281:286	arg1	addition					227:234	addition	227:234	addition	227:234	In addition, unlike most viruses, these organisms do not hijack the biosynthetic machinery of the host to make glycocoproteins; instead, they produce their own carbohydrate-processing enzymes.					
30280442	0	54	theme	Chlorella	56:64	arg1	Glycans					81:87	Chlorella Virus N-Linked Glycans	56:87	Chlorella Virus N-Linked Glycans	56:87	Synthesis of the Highly Branched Hexasaccharide Core of Chlorella Virus N-Linked Glycans.					
30280442	3	55	theme	probe	548:552	arg1	molecules					554:562	probe molecules	548:562	probe molecules	548:562	A better understanding of the function and assembly of these fascinating and structurally-unprecedented glycans requires access to probe molecules.					
31094418	8	0	theme	certain	1528:1534	arg1	conditions					1566:1575	certain human allergic and autoimmune conditions	1528:1575	certain human allergic and autoimmune conditions	1528:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	4	1	theme	advanced	682:689	arg1	chromatography					725:738	more advanced analytical tools-nano-flow liquid chromatography	677:738	more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to	677:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	8	2	theme	conditions	1566:1575	arg1	treatment					1515:1523	the treatment	1511:1523	the treatment of certain human allergic and autoimmune conditions	1511:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	8	3	theme	molecule	1438:1445	arg1	effects					1425:1431	the potent immunomodulatory effects	1397:1431	the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions	1397:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	8	4	theme	immunomodulatory	1408:1423	arg1	effects					1425:1431	the potent immunomodulatory effects	1397:1431	the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions	1397:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	3	5	dep	ES-62	563:567	arg1	sites					602:606	four potential N-glycosylation sites	571:606	ES-62's four potential N-glycosylation sites	563:606	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	6	6	from	PC	1162:1163	arg1	core					1192:1195	the N-glycan chitobiose core	1168:1195	the N-glycan chitobiose core	1168:1195	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	8	7	theme	autoimmune	1555:1564	arg1	conditions					1566:1575	certain human allergic and autoimmune conditions	1528:1575	certain human allergic and autoimmune conditions	1528:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	0	8	theme	worm	100:103	arg1	viteae					123:128	the parasitic worm Acanthocheilonema viteae	86:128	the parasitic worm Acanthocheilonema viteae	86:128	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	3	9	theme	PC-modified	620:630	arg1	glycans					632:638	the PC-modified glycans	616:638	the PC-modified glycans	616:638	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	3	10	contain	carries	608:614	arg2	glycans					632:638	the PC-modified glycans	616:638	the PC-modified glycans	616:638	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	3	10	contain	carries	608:614	arg1	time					549:552	this time	544:552	this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans	544:638	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	7	11	theme	immune	1354:1359	arg1	system					1361:1366	the immune system	1350:1366	the immune system	1350:1366	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	4	12	located	found	829:833	arg2	glycans					817:823	PC-modified glycans	805:823	PC-modified glycans	805:823	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	4	12	located	found	829:833	arg1	sites					873:877	all four potential N-glycosylation sites	838:877	all four potential N-glycosylation sites	838:877	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	0	13	theme	parasitic	90:98	arg1	viteae					123:128	the parasitic worm Acanthocheilonema viteae	86:128	the parasitic worm Acanthocheilonema viteae	86:128	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	6	14	theme	terminal	1212:1219	arg1	GlcNAc					1221:1226	terminal GlcNAc	1212:1226	terminal GlcNAc	1212:1226	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	7	15	theme	system	1361:1366	arg1	molecules					1337:1345	molecules	1337:1345	molecules	1337:1345	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	7	15	theme	system	1361:1366	arg1	cells					1327:1331	cells	1327:1331	cells	1327:1331	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	1	16	theme	posttranslational	291:307	arg1	decoration					309:318	posttranslational decoration	291:318	posttranslational decoration by phosphorylcholine (PC)	291:344	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	0	17	theme	viteae	123:128	arg1	product					75:81	The major secreted product	56:81	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	5	18	theme	PC	928:929	arg1	groups					931:936	up to two PC groups	918:936	up to two PC groups	918:936	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	4	19	theme	PC-modified	805:815	arg1	glycans					817:823	PC-modified glycans	805:823	PC-modified glycans	805:823	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	7	20	theme	PC	1259:1260	arg1	majority					1247:1254	the majority	1243:1254	the majority of PC	1243:1260	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	5	21	dep	five	1021:1024	arg1	to					1018:1019	to	1018:1019	to	1018:1019	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	0	22	theme	Acanthocheilonema	105:121	arg1	viteae					123:128	the parasitic worm Acanthocheilonema viteae	86:128	the parasitic worm Acanthocheilonema viteae	86:128	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	8	23	theme	human	1536:1540	arg1	conditions					1566:1575	certain human allergic and autoimmune conditions	1528:1575	certain human allergic and autoimmune conditions	1528:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	2	24	theme	mass	451:454	arg1	spectrometry					456:467	fast atom bombardment mass spectrometry	429:467	fast atom bombardment mass spectrometry	429:467	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	1	25	theme	parasitic	174:182	arg1	viteae					220:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	0	26	theme	glycoproteomic	14:27	arg1	characterization					29:44	Site-specific glycoproteomic characterization	0:44	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	7	27	located	detected	1265:1272	arg1	GlcNAc					1286:1291	terminal GlcNAc	1277:1291	terminal GlcNAc	1277:1291	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	7	27	located	detected	1265:1272	arg2	majority					1247:1254	the majority	1243:1254	the majority of PC	1243:1260	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	6	28	gly	glycosylation	1087:1099	arg2	sites					1101:1105	the four glycosylation sites	1078:1105	the four glycosylation sites	1078:1105	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	6	28	gly	glycosylation	1087:1099	arg2	four					1082:1085	four	1082:1085	four	1082:1085	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	4	29	theme	present	648:654	arg1	study					656:660	the present study	644:660	the present study	644:660	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	4	30	theme	high-definition	745:759	arg1	spectrometry-to					779:793	high-definition electrospray mass spectrometry-to	745:793	high-definition electrospray mass spectrometry-to	745:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	1	31	theme	filarial	184:191	arg1	viteae					220:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	0	32	theme	Site-specific	0:12	arg1	characterization					29:44	Site-specific glycoproteomic characterization	0:44	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	1	33	theme	decoration	309:318	arg1	consequence					276:286	a consequence	274:286	a consequence of posttranslational decoration by phosphorylcholine (PC)	274:344	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	5	34	theme	earlier	890:896	arg1	studies					898:904	our earlier studies	886:904	our earlier studies	886:904	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	1	35	theme	nematode	193:200	arg1	viteae					220:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	2	36	theme	bombardment	439:449	arg1	spectrometry					456:467	fast atom bombardment mass spectrometry	429:467	fast atom bombardment mass spectrometry	429:467	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	5	37	theme	PC	1026:1027	arg1	groups					1029:1034	up to five PC groups	1015:1034	up to five PC groups	1015:1034	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	4	38	theme	mass	774:777	arg1	spectrometry-to					779:793	high-definition electrospray mass spectrometry-to	745:793	high-definition electrospray mass spectrometry-to	745:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	6	39	theme	chitobiose	1181:1190	arg1	core					1192:1195	the N-glycan chitobiose core	1168:1195	the N-glycan chitobiose core	1168:1195	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	1	40	theme	Acanthocheilonema	202:218	arg1	viteae					220:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	the parasitic filarial nematode Acanthocheilonema viteae	170:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	2	41	theme	atom	434:437	arg1	spectrometry					456:467	fast atom bombardment mass spectrometry	429:467	fast atom bombardment mass spectrometry	429:467	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	7	42	dep	cells	1327:1331	arg1	the					1323:1325	the	1323:1325	the	1323:1325	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	4	43	dep	employ	670:675	arg1	show					795:798	show	795:798	employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites	670:877	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	8	44	theme	Such	1369:1372	arg1	expression					1374:1383	Such expression	1369:1383	Such expression	1369:1383	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	4	45	theme	electrospray	761:772	arg1	spectrometry-to					779:793	high-definition electrospray mass spectrometry-to	745:793	high-definition electrospray mass spectrometry-to	745:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	1	46	theme	viteae	220:225	arg1	product					159:165	the major secreted product	140:165	the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae	140:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	1	46	theme	viteae	220:225	arg1	ES-62					131:135	ES-62	131:135	ES-62	131:135	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	8	47	theme	therapeutic	1486:1496	arg1	potential					1498:1506	significant therapeutic potential	1474:1506	significant therapeutic potential	1474:1506	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	5	48	with	N-glycans	1000:1008	arg1	groups					1029:1034	up to five PC groups	1015:1034	up to five PC groups	1015:1034	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	3	49	theme	potential	576:584	arg1	sites					602:606	four potential N-glycosylation sites	571:606	ES-62's four potential N-glycosylation sites	563:606	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	3	50	gly	N-glycosylation	586:600	arg2	sites					602:606	four potential N-glycosylation sites	571:606	ES-62's four potential N-glycosylation sites	563:606	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	3	50	gly	N-glycosylation	586:600	arg2	four					571:574	four	571:574	four	571:574	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	0	51	theme	ES-62	49:53	arg1	characterization					29:44	Site-specific glycoproteomic characterization	0:44	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	3	52	theme	N-glycosylation	586:600	arg1	sites					602:606	four potential N-glycosylation sites	571:606	ES-62's four potential N-glycosylation sites	563:606	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	0	53	theme	major	60:64	arg1	product					75:81	The major secreted product	56:81	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	2	54	link	N-linked	401:408	arg1	glycans					410:416	N-linked glycans	401:416	N-linked glycans	401:416	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	4	55	theme	N-glycosylation	857:871	arg1	sites					873:877	all four potential N-glycosylation sites	838:877	all four potential N-glycosylation sites	838:877	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	5	56	dep	two	924:926	arg1	to					921:922	to	921:922	to	921:922	Also, our earlier studies showed that up to two PC groups were detected per glycan, and we are now able to characterize N-glycans with up to five PC groups.					
31094418	4	57	theme	tools-nano-flow	702:716	arg1	chromatography					725:738	more advanced analytical tools-nano-flow liquid chromatography	677:738	more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to	677:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	1	58	contain	has	231:233	arg1	product					159:165	the major secreted product	140:165	the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae	140:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	1	58	contain	has	231:233	arg1	ES-62					131:135	ES-62	131:135	ES-62	131:135	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	1	58	contain	has	231:233	arg2	activities					260:269	potent anti-inflammatory activities	235:269	potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC)	235:344	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	4	59	theme	potential	847:855	arg1	sites					873:877	all four potential N-glycosylation sites	838:877	all four potential N-glycosylation sites	838:877	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	4	60	theme	analytical	691:700	arg1	chromatography					725:738	more advanced analytical tools-nano-flow liquid chromatography	677:738	more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to	677:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	3	61	from	time	549:552	arg1	unknown					533:539	unknown	533:539	unknown	533:539	However, it was unknown at this time which of ES-62's four potential N-glycosylation sites carries the PC-modified glycans.					
31094418	8	62	theme	significant	1474:1484	arg1	potential					1498:1506	significant therapeutic potential	1474:1506	significant therapeutic potential	1474:1506	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	6	63	theme	first	1133:1137	arg1	time					1139:1142	the first time	1129:1142	the first time	1129:1142	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	6	64	theme	N-glycan	1172:1179	arg1	core					1192:1195	the N-glycan chitobiose core	1168:1195	the N-glycan chitobiose core	1168:1195	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	6	65	theme	sites	1101:1105	arg1	sites					1101:1105	the four glycosylation sites	1078:1105	the four glycosylation sites	1078:1105	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	6	65	theme	sites	1101:1105	arg1	three					1069:1073	three	1069:1073	three	1069:1073	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	6	66	theme	glycosylation	1087:1099	arg1	sites					1101:1105	the four glycosylation sites	1078:1105	the four glycosylation sites	1078:1105	The number per glycan varies in three of the four glycosylation sites, and in addition, for the first time, we have detected PC on the N-glycan chitobiose core in addition to terminal GlcNAc.					
31094418	1	67	theme	potent	235:240	arg1	activities					260:269	potent anti-inflammatory activities	235:269	potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC)	235:344	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	2	68	attach	attached	389:396	arg2	PC					382:383	ES-62's PC	374:383	ES-62's PC	374:383	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	2	68	attach	attached	389:396	arg1	glycans					410:416	N-linked glycans	401:416	N-linked glycans	401:416	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	0	69	dep	characterization	29:44	arg1	product					75:81	The major secreted product	56:81	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	4	70	theme	liquid	718:723	arg1	chromatography					725:738	more advanced analytical tools-nano-flow liquid chromatography	677:738	more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to	677:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	1	71	theme	anti-inflammatory	242:258	arg1	activities					260:269	potent anti-inflammatory activities	235:269	potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC)	235:344	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	2	72	theme	glycans	508:514	arg1	structure					491:499	the structure	487:499	the structure of the glycans	487:514	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	8	73	theme	potent	1401:1406	arg1	effects					1425:1431	the potent immunomodulatory effects	1397:1431	the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions	1397:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	7	74	theme	terminal	1277:1284	arg1	GlcNAc					1286:1291	terminal GlcNAc	1277:1291	terminal GlcNAc	1277:1291	Nevertheless, the majority of PC is detected on terminal GlcNAc, enabling it to interact with the cells and molecules of the immune system.					
31094418	2	75	theme	fast	429:432	arg1	spectrometry					456:467	fast atom bombardment mass spectrometry	429:467	fast atom bombardment mass spectrometry	429:467	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	1	76	theme	major	144:148	arg1	product					159:165	the major secreted product	140:165	the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae	140:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	1	76	theme	major	144:148	arg1	ES-62					131:135	ES-62	131:135	ES-62	131:135	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	2	77	theme	N-linked	401:408	arg1	glycans					410:416	N-linked glycans	401:416	N-linked glycans	401:416	Previously, we showed that ES-62's PC was attached to N-linked glycans, and using fast atom bombardment mass spectrometry, we characterized the structure of the glycans.					
31094418	0	78	theme	secreted	66:73	arg1	product					75:81	The major secreted product	56:81	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.	0:129	Site-specific glycoproteomic characterization of ES-62: The major secreted product of the parasitic worm Acanthocheilonema viteae.					
31094418	4	79	with	chromatography	725:738	arg1	spectrometry-to					779:793	high-definition electrospray mass spectrometry-to	745:793	high-definition electrospray mass spectrometry-to	745:793	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	4	80	gly	N-glycosylation	857:871	arg2	sites					873:877	all four potential N-glycosylation sites	838:877	all four potential N-glycosylation sites	838:877	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	4	80	gly	N-glycosylation	857:871	arg2	four					842:845	four	842:845	four	842:845	In the present study, we now employ more advanced analytical tools-nano-flow liquid chromatography with high-definition electrospray mass spectrometry-to show that PC-modified glycans are found at all four potential N-glycosylation sites.					
31094418	8	81	contain	have	1469:1472	arg1	molecule					1438:1445	a molecule	1436:1445	a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions	1436:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	8	81	contain	have	1469:1472	arg2	potential					1498:1506	significant therapeutic potential	1474:1506	significant therapeutic potential	1474:1506	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
31094418	1	82	theme	secreted	150:157	arg1	product					159:165	the major secreted product	140:165	the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae	140:225	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	1	82	theme	secreted	150:157	arg1	ES-62					131:135	ES-62	131:135	ES-62	131:135	ES-62 is the major secreted product of the parasitic filarial nematode Acanthocheilonema viteae and has potent anti-inflammatory activities as a consequence of posttranslational decoration by phosphorylcholine (PC).					
31094418	8	83	theme	allergic	1542:1549	arg1	conditions					1566:1575	certain human allergic and autoimmune conditions	1528:1575	certain human allergic and autoimmune conditions	1528:1575	Such expression may explain the potent immunomodulatory effects of a molecule that is considered to have significant therapeutic potential in the treatment of certain human allergic and autoimmune conditions.					
30413744	1	0	with	infection	148:156	arg1	nematodes					172:180	filarial nematodes	163:180	filarial nematodes	163:180	Filariases are diseases caused by infection with filarial nematodes and transmitted by insect vectors.					
30413744	9	1	theme	infection	1319:1327	arg1	months					1309:1314	the first 6 months	1297:1314	the first 6 months of infection in each dog analyzed	1297:1348	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	4	2	theme	pathological	590:601	arg1	infections					603:612	both pathological infections	585:612	both pathological infections	585:612	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	4	3	from	changes	618:624	arg1	biology					639:645	mammalian biology	629:645	mammalian biology	629:645	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	10	4	theme	parasitic	1453:1461	arg1	infection					1463:1471	a parasitic infection	1451:1471	a parasitic infection	1451:1471	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	9	5	theme	canine	1203:1208	arg1	A2G2S2					1243:1248	biantennary, disialylated A2G2S2	1217:1248	biantennary, disialylated A2G2S2	1217:1248	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	5	theme	canine	1203:1208	arg1	serum					1210:1214	canine serum	1203:1214	canine serum (biantennary, disialylated A2G2S2)	1203:1249	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	8	6	theme	individual	1046:1055	arg1	structures					1064:1073	individual glycan structures	1046:1073	individual glycan structures that change significantly in their relative abundance during infection	1046:1144	We also identify individual glycan structures that change significantly in their relative abundance during infection.					
30413744	0	7	theme	parasite	84:91	arg1	immitis					105:111	the heartworm parasite Dirofilaria immitis	70:111	the heartworm parasite Dirofilaria immitis	70:111	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	0	8	from	Changes	0:6	arg1	N-glycosylation					24:38	canine serum N-glycosylation	11:38	canine serum N-glycosylation	11:38	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	10	9	theme	longitudinal	1369:1380	arg1	This					1351:1354	This	1351:1354	This	1351:1354	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	10	9	theme	longitudinal	1369:1380	arg1	study					1382:1386	the first longitudinal study	1359:1386	the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection	1359:1471	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	2	10	theme	other	297:301	arg1	carnivores					303:312	other carnivores	297:312	other carnivores	297:312	The filarial roundworm Dirofilaria immitis causes heartworm disease in dogs and other carnivores.					
30413744	0	11	theme	heartworm	74:82	arg1	immitis					105:111	the heartworm parasite Dirofilaria immitis	70:111	the heartworm parasite Dirofilaria immitis	70:111	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	6	12	theme	infection	905:913	arg1	study					915:919	a longitudinal infection study	890:919	a longitudinal infection study	890:919	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	8	13	theme	glycan	1057:1062	arg1	structures					1064:1073	individual glycan structures	1046:1073	individual glycan structures that change significantly in their relative abundance during infection	1046:1144	We also identify individual glycan structures that change significantly in their relative abundance during infection.					
30413744	0	14	with	infection	55:63	arg1	immitis					105:111	the heartworm parasite Dirofilaria immitis	70:111	the heartworm parasite Dirofilaria immitis	70:111	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	1	15	dep	diseases	129:136	arg1	transmitted					186:196	transmitted	186:196	transmitted by insect vectors	186:214	Filariases are diseases caused by infection with filarial nematodes and transmitted by insect vectors.					
30413744	1	15	dep	diseases	129:136	arg1	caused					138:143	caused	138:143	caused by infection with filarial nematodes	138:180	Filariases are diseases caused by infection with filarial nematodes and transmitted by insect vectors.					
30413744	5	16	from	changes	717:723	arg1	profiles					754:761	the serum N-glycosylation profiles	728:761	the serum N-glycosylation profiles of dogs infected with D. immitis	728:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	3	17	theme	Onchocerca	348:357	arg1	volvulus					359:366	Onchocerca volvulus	348:366	Onchocerca volvulus	348:366	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	17	theme	Onchocerca	348:357	arg1	bancrofti					380:388	Wuchereria bancrofti	369:388	Wuchereria bancrofti	369:388	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	17	theme	Onchocerca	348:357	arg1	malayi					401:406	Brugia malayi	394:406	Brugia malayi	394:406	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	6	18	theme	longitudinal	892:903	arg1	study					915:919	a longitudinal infection study	890:919	a longitudinal infection study	890:919	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	4	19	theme	due	647:649	arg1	infections					603:612	both pathological infections	585:612	both pathological infections	585:612	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	10	20	theme	first	1363:1367	arg1	This					1351:1354	This	1351:1354	This	1351:1354	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	10	20	theme	first	1363:1367	arg1	study					1382:1386	the first longitudinal study	1359:1386	the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection	1359:1471	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	1	21	theme	filarial	163:170	arg1	nematodes					172:180	filarial nematodes	163:180	filarial nematodes	163:180	Filariases are diseases caused by infection with filarial nematodes and transmitted by insect vectors.					
30413744	0	22	theme	Dirofilaria	93:103	arg1	immitis					105:111	the heartworm parasite Dirofilaria immitis	70:111	the heartworm parasite Dirofilaria immitis	70:111	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	7	23	theme	core	951:954	arg1	fucosylation					956:967	core fucosylation	951:967	core fucosylation	951:967	Overall, galactosylation and core fucosylation increase, while sialylation decreases in infected dog sera.					
30413744	3	24	dep	D.	315:316	arg1	immitis					318:324	D. immitis	315:324	D. immitis	315:324	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	25	theme	Brugia	394:399	arg1	volvulus					359:366	Onchocerca volvulus	348:366	Onchocerca volvulus	348:366	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	25	theme	Brugia	394:399	arg1	malayi					401:406	Brugia malayi	394:406	Brugia malayi	394:406	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	5	26	theme	D.	785:786	arg1	immitis					788:794	D. immitis	785:794	D. immitis	785:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	10	27	theme	infection	1463:1471	arg1	progression					1436:1446	progression	1436:1446	progression of a parasitic infection	1436:1471	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	5	28	theme	dogs	766:769	arg1	profiles					754:761	the serum N-glycosylation profiles	728:761	the serum N-glycosylation profiles of dogs infected with D. immitis	728:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	5	29	gly	N-glycosylation	738:752	arg1	dogs					766:769	dogs	766:769	dogs infected with D. immitis	766:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	9	30	from	dog	1337:1339	arg1	months					1309:1314	the first 6 months	1297:1314	the first 6 months of infection in each dog analyzed	1297:1348	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	6	31	theme	serum	830:834	arg1	analysis					818:825	analysis	818:825	analysis of serum	818:834	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	6	32	with	dogs	841:844	arg1	infections					870:879	established patent infections	851:879	established patent infections	851:879	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	3	33	theme	river	437:441	arg1	blindness					443:451	river blindness	437:451	river blindness	437:451	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	33	theme	river	437:441	arg1	onchocerciasis					421:434	onchocerciasis	421:434	onchocerciasis (river blindness)	421:452	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	0	34	theme	serum	18:22	arg1	N-glycosylation					24:38	canine serum N-glycosylation	11:38	canine serum N-glycosylation	11:38	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	5	35	theme	significant	705:715	arg1	changes					717:723	significant changes	705:723	significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis	705:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	9	36	theme	biantennary	1217:1227	arg1	A2G2S2					1243:1248	biantennary, disialylated A2G2S2	1217:1248	biantennary, disialylated A2G2S2	1217:1248	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	36	theme	biantennary	1217:1227	arg1	serum					1210:1214	canine serum	1203:1214	canine serum (biantennary, disialylated A2G2S2)	1203:1249	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	10	37	theme	mammalian	1407:1415	arg1	N-glycome					1423:1431	mammalian serum N-glycome	1407:1431	mammalian serum N-glycome	1407:1431	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	0	38	theme	canine	11:16	arg1	N-glycosylation					24:38	canine serum N-glycosylation	11:38	canine serum N-glycosylation	11:38	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	2	39	from	disease	277:283	arg1	dogs					288:291	dogs	288:291	dogs	288:291	The filarial roundworm Dirofilaria immitis causes heartworm disease in dogs and other carnivores.					
30413744	2	39	from	disease	277:283	arg1	carnivores					303:312	other carnivores	297:312	other carnivores	297:312	The filarial roundworm Dirofilaria immitis causes heartworm disease in dogs and other carnivores.					
30413744	1	40	theme	insect	201:206	arg1	vectors					208:214	insect vectors	201:214	insect vectors	201:214	Filariases are diseases caused by infection with filarial nematodes and transmitted by insect vectors.					
30413744	9	41	from	months	1309:1314	arg1	dog					1337:1339	each dog	1332:1339	each dog analyzed	1332:1348	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	42	from	abundance	1160:1168	arg1	A2G2S2					1243:1248	biantennary, disialylated A2G2S2	1217:1248	biantennary, disialylated A2G2S2	1217:1248	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	42	from	abundance	1160:1168	arg1	serum					1210:1214	canine serum	1203:1214	canine serum (biantennary, disialylated A2G2S2)	1203:1249	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	4	43	theme	lifestyle	670:678	arg1	choices					680:686	lifestyle choices	670:686	lifestyle choices	670:686	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	4	44	from	infections	603:612	arg1	biology					639:645	mammalian biology	629:645	mammalian biology	629:645	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	9	45	from	infection	1319:1327	arg1	dog					1337:1339	each dog	1332:1339	each dog analyzed	1332:1348	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	3	46	theme	lymphatic	458:466	arg1	elephantiasis					480:492	elephantiasis	480:492	elephantiasis	480:492	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	3	46	theme	lymphatic	458:466	arg1	filariasis					468:477	lymphatic filariasis	458:477	lymphatic filariasis (elephantiasis)	458:493	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	5	47	theme	serum	732:736	arg1	profiles					754:761	the serum N-glycosylation profiles	728:761	the serum N-glycosylation profiles of dogs infected with D. immitis	728:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	6	48	theme	established	851:861	arg1	infections					870:879	established patent infections	851:879	established patent infections	851:879	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	2	49	theme	filarial	221:228	arg1	roundworm					230:238	The filarial roundworm	217:238	The filarial roundworm Dirofilaria immitis	217:258	The filarial roundworm Dirofilaria immitis causes heartworm disease in dogs and other carnivores.					
30413744	10	50	from	changes	1396:1402	arg1	N-glycome					1423:1431	mammalian serum N-glycome	1407:1431	mammalian serum N-glycome	1407:1431	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	6	51	from	dogs	841:844	arg1	analysis					818:825	analysis	818:825	analysis of serum	818:834	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	8	52	theme	relative	1110:1117	arg1	abundance					1119:1127	their relative abundance	1104:1127	their relative abundance	1104:1127	We also identify individual glycan structures that change significantly in their relative abundance during infection.					
30413744	9	53	theme	percentage	1272:1281	arg1	points					1283:1288	over 10 percentage points	1264:1288	over 10 percentage points	1264:1288	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	6	54	theme	patent	863:868	arg1	infections					870:879	established patent infections	851:879	established patent infections	851:879	Our data derive from analysis of serum from dogs with established patent infections and from a longitudinal infection study.					
30413744	4	55	theme	Serum	542:546	arg1	N-glycosylation					548:562	Serum N-glycosylation	542:562	Serum N-glycosylation	542:562	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	9	56	dep	biantennary	1217:1227	arg1	disialylated					1230:1241	disialylated	1230:1241	disialylated	1230:1241	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	57	theme	dominant	1182:1189	arg1	N-glycan					1191:1198	the most dominant N-glycan	1173:1198	the most dominant N-glycan	1173:1198	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	2	58	theme	heartworm	267:275	arg1	disease					277:283	heartworm disease	267:283	heartworm disease in dogs and other carnivores	267:312	The filarial roundworm Dirofilaria immitis causes heartworm disease in dogs and other carnivores.					
30413744	7	59	theme	infected	1010:1017	arg1	sera					1023:1026	infected dog sera	1010:1026	infected dog sera	1010:1026	Overall, galactosylation and core fucosylation increase, while sialylation decreases in infected dog sera.					
30413744	5	60	theme	N-glycosylation	738:752	arg1	profiles					754:761	the serum N-glycosylation profiles	728:761	the serum N-glycosylation profiles of dogs infected with D. immitis	728:794	Here, we report significant changes in the serum N-glycosylation profiles of dogs infected with D. immitis.					
30413744	0	61	theme	infection	55:63	arg1	result					45:50	a result	43:50	a result of infection with the heartworm parasite Dirofilaria immitis	43:111	Changes in canine serum N-glycosylation as a result of infection with the heartworm parasite Dirofilaria immitis.					
30413744	4	62	theme	normal	654:659	arg1	aging					661:665	normal aging	654:665	normal aging	654:665	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
30413744	7	63	theme	dog	1019:1021	arg1	sera					1023:1026	infected dog sera	1010:1026	infected dog sera	1010:1026	Overall, galactosylation and core fucosylation increase, while sialylation decreases in infected dog sera.					
30413744	3	64	theme	neglected	513:521	arg1	diseases					532:539	neglected tropical diseases	513:539	neglected tropical diseases	513:539	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	10	65	theme	serum	1417:1421	arg1	N-glycome					1423:1431	mammalian serum N-glycome	1407:1431	mammalian serum N-glycome	1407:1431	This is the first longitudinal study linking changes in mammalian serum N-glycome to progression of a parasitic infection.					
30413744	9	66	theme	first	1301:1305	arg1	months					1309:1314	the first 6 months	1297:1314	the first 6 months of infection in each dog analyzed	1297:1348	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	9	67	theme	N-glycan	1191:1198	arg1	abundance					1160:1168	the abundance	1156:1168	the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2)	1156:1249	Notably, the abundance of the most dominant N-glycan in canine serum (biantennary, disialylated A2G2S2) decreases by over 10 percentage points during the first 6 months of infection in each dog analyzed.					
30413744	3	68	theme	tropical	523:530	arg1	diseases					532:539	neglected tropical diseases	513:539	neglected tropical diseases	513:539	D. immitis is closely related to Onchocerca volvulus, Wuchereria bancrofti and Brugia malayi, which cause onchocerciasis (river blindness) and lymphatic filariasis (elephantiasis) in humans and are neglected tropical diseases.					
30413744	4	69	theme	mammalian	629:637	arg1	biology					639:645	mammalian biology	629:645	mammalian biology	629:645	Serum N-glycosylation is very sensitive to both pathological infections and changes in mammalian biology due to normal aging or lifestyle choices.					
31126879	0	0	theme	HIV	74:76	arg1	Envelope					78:85	the HIV Envelope	70:85	the HIV Envelope	70:85	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	6	1	theme	Administrating	910:923	arg1	SF12					925:928	Administrating SF12	910:928	Administrating SF12 to HIV-1-infected humanized mice	910:961	Administrating SF12 to HIV-1-infected humanized mice suppressed viremia and selected for viruses lacking the N448gp120 glycan.					
31126879	3	2	theme	V3-glycan	453:461	arg1	bNAbs					463:467	V3-glycan bNAbs	453:467	V3-glycan bNAbs	453:467	SF12 recognized a glycan-dominated epitope on Env's silent face and was potent against clade AE viruses, which are poorly covered by V3-glycan bNAbs.					
31126879	4	3	theme	A 3.3Å	470:475	arg1	structure					485:493	A 3.3Å cryo-EM structure	470:493	A 3.3Å cryo-EM structure of a SF12-Env trimer complex	470:522	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	5	4	theme	intra-Env	878:886	arg1	heterogeneity					895:907	intra-Env glycan heterogeneity	878:907	intra-Env glycan heterogeneity	878:907	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	5	5	theme	glycan	888:893	arg1	heterogeneity					895:907	intra-Env glycan heterogeneity	878:907	intra-Env glycan heterogeneity	878:907	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	4	6	theme	additional	531:540	arg1	contacts					542:549	additional contacts	531:549	additional contacts to Env protein residues	531:573	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	7	link	donor-derived	628:640	arg1	antibody					653:660	the only other known donor-derived silentface antibody	607:660	the only other known donor-derived silentface antibody	607:660	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	7	link	donor-derived	628:640	arg1	VRC-PG05					597:604	VRC-PG05	597:604	VRC-PG05	597:604	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	8	theme	Env	554:556	arg1	residues					566:573	Env protein residues	554:573	Env protein residues	554:573	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	0	9	theme	Envelope	78:85	arg1	Face					62:65	the Silent Face	51:65	the Silent Face of the HIV Envelope	51:85	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	2	10	from	donor	313:317	arg1	breadth					284:290	up to 62% neutralization breadth	259:290	up to 62% neutralization breadth from an HIV-infected donor	259:317	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	4	11	theme	increased	681:689	arg1	SF12					674:677	SF12's increased neutralization breadth, potency, and resistance to Env mutation routes	674:760	SF12	674:677	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	12	theme	Env	742:744	arg1	routes					755:760	Env mutation routes	742:760	Env mutation routes	742:760	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	5	13	theme	Env	849:851	arg1	protomers					853:861	Env protomers	849:861	Env protomers	849:861	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	5	14	theme	Asymmetric	763:772	arg1	binding					774:780	Asymmetric binding	763:780	Asymmetric binding of SF12	763:788	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	3	15	theme	clade	407:411	arg1	viruses					416:422	clade AE viruses	407:422	clade AE viruses	407:422	SF12 recognized a glycan-dominated epitope on Env's silent face and was potent against clade AE viruses, which are poorly covered by V3-glycan bNAbs.					
31126879	7	16	theme	vaccine	1172:1178	arg1	development					1180:1190	vaccine development	1172:1190	vaccine development	1172:1190	Effective bNAbs can therefore be raised against HIV-1 Env's silent face, suggesting their potential for HIV-1 prevention, therapy, and vaccine development.					
31126879	4	17	theme	complex	516:522	arg1	structure					485:493	A 3.3Å cryo-EM structure	470:493	A 3.3Å cryo-EM structure of a SF12-Env trimer complex	470:522	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	18	theme	known	622:626	arg1	antibody					653:660	the only other known donor-derived silentface antibody	607:660	the only other known donor-derived silentface antibody	607:660	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	18	theme	known	622:626	arg1	VRC-PG05					597:604	VRC-PG05	597:604	VRC-PG05	597:604	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	0	19	theme	Broad	0:4	arg1	Antibodies					30:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	4	20	theme	trimer	509:514	arg1	complex					516:522	a SF12-Env trimer complex	498:522	a SF12-Env trimer complex	498:522	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	7	21	theme	silent	1097:1102	arg1	face					1104:1107	HIV-1 Env's silent face	1085:1107	HIV-1 Env's silent face	1085:1107	Effective bNAbs can therefore be raised against HIV-1 Env's silent face, suggesting their potential for HIV-1 prevention, therapy, and vaccine development.					
31126879	2	22	theme	neutralization	269:282	arg1	breadth					284:290	up to 62% neutralization breadth	259:290	up to 62% neutralization breadth from an HIV-infected donor	259:317	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	1	23	theme	vaccine	164:170	arg1	design					172:177	vaccine design	164:177	vaccine design	164:177	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	3	24	theme	glycan-dominated	338:353	arg1	epitope					355:361	a glycan-dominated epitope	336:361	a glycan-dominated epitope	336:361	SF12 recognized a glycan-dominated epitope on Env's silent face and was potent against clade AE viruses, which are poorly covered by V3-glycan bNAbs.					
31126879	4	25	theme	SF12-Env	500:507	arg1	complex					516:522	a SF12-Env trimer complex	498:522	a SF12-Env trimer complex	498:522	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	7	26	theme	HIV-1	1085:1089	arg1	face					1104:1107	HIV-1 Env's silent face	1085:1107	HIV-1 Env's silent face	1085:1107	Effective bNAbs can therefore be raised against HIV-1 Env's silent face, suggesting their potential for HIV-1 prevention, therapy, and vaccine development.					
31126879	4	27	dep	SF12	674:677	arg1	neutralization breadth					691:712	neutralization breadth	691:712	neutralization breadth	691:712	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	0	28	theme	Potent	10:15	arg1	Antibodies					30:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	1	29	theme	neutralizing	96:107	arg1	bNAbs					121:125	bNAbs	121:125	bNAbs	121:125	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	1	29	theme	neutralizing	96:107	arg1	antibodies					109:118	Broadly neutralizing antibodies	88:118	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env)	88:155	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	5	30	theme	SF12	785:788	arg1	binding					774:780	Asymmetric binding	763:780	Asymmetric binding of SF12	763:788	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	2	31	dep	62	265:266	arg1	to					262:263	to	262:263	to	262:263	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	5	32	theme	distinct	810:817	arg1	conformations					828:840	distinct N-glycan conformations	810:840	distinct N-glycan conformations across Env protomers	810:861	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	3	33	theme	AE	413:414	arg1	viruses					416:422	clade AE viruses	407:422	clade AE viruses	407:422	SF12 recognized a glycan-dominated epitope on Env's silent face and was potent against clade AE viruses, which are poorly covered by V3-glycan bNAbs.					
31126879	0	34	theme	Neutralizing	17:28	arg1	Antibodies					30:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies	0:39	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	1	35	theme	potential	187:195	arg1	agents					209:214	potential therapeutic agents	187:214	potential therapeutic agents	187:214	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	0	36	theme	Silent	55:60	arg1	Face					62:65	the Silent Face	51:65	the Silent Face of the HIV Envelope	51:85	Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.					
31126879	4	37	theme	cryo-EM	477:483	arg1	structure					485:493	A 3.3Å cryo-EM structure	470:493	A 3.3Å cryo-EM structure of a SF12-Env trimer complex	470:522	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	7	38	theme	Effective	1037:1045	arg1	bNAbs					1047:1051	Effective bNAbs	1037:1051	Effective bNAbs	1037:1051	Effective bNAbs can therefore be raised against HIV-1 Env's silent face, suggesting their potential for HIV-1 prevention, therapy, and vaccine development.					
31126879	2	39	dep	%	267:267	arg1	62					265:266	62	265:266	62	265:266	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	3	40	theme	silent	372:377	arg1	face					379:382	Env's silent face	366:382	Env's silent face	366:382	SF12 recognized a glycan-dominated epitope on Env's silent face and was potent against clade AE viruses, which are poorly covered by V3-glycan bNAbs.					
31126879	6	41	theme	HIV-1-infected	933:946	arg1	mice					958:961	HIV-1-infected humanized mice	933:961	HIV-1-infected humanized mice	933:961	Administrating SF12 to HIV-1-infected humanized mice suppressed viremia and selected for viruses lacking the N448gp120 glycan.					
31126879	2	42	theme	HIV-infected	300:311	arg1	donor					313:317	an HIV-infected donor	297:317	an HIV-infected donor	297:317	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	2	43	theme	related	240:246	arg1	bNAbs					248:252	related bNAbs	240:252	related bNAbs	240:252	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	2	44	dep	breadth	284:290	arg1	%					267:267	%	267:267	%	267:267	We identified SF12 and related bNAbs with up to 62% neutralization breadth from an HIV-infected donor.					
31126879	1	45	theme	therapeutic	197:207	arg1	agents					209:214	potential therapeutic agents	187:214	potential therapeutic agents	187:214	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	4	46	theme	mutation	746:753	arg1	routes					755:760	Env mutation routes	742:760	Env mutation routes	742:760	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	47	theme	donor-derived	628:640	arg1	antibody					653:660	the only other known donor-derived silentface antibody	607:660	the only other known donor-derived silentface antibody	607:660	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	47	theme	donor-derived	628:640	arg1	VRC-PG05					597:604	VRC-PG05	597:604	VRC-PG05	597:604	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	48	theme	protein	558:564	arg1	residues					566:573	Env protein residues	554:573	Env protein residues	554:573	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	5	49	theme	N-glycan	819:826	arg1	conformations					828:840	distinct N-glycan conformations	810:840	distinct N-glycan conformations across Env protomers	810:861	Asymmetric binding of SF12 was associated with distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity.					
31126879	4	50	theme	silentface	642:651	arg1	antibody					653:660	the only other known donor-derived silentface antibody	607:660	the only other known donor-derived silentface antibody	607:660	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	4	50	theme	silentface	642:651	arg1	VRC-PG05					597:604	VRC-PG05	597:604	VRC-PG05	597:604	A 3.3Å cryo-EM structure of a SF12-Env trimer complex showed additional contacts to Env protein residues by SF12 compared with VRC-PG05, the only other known donor-derived silentface antibody, explaining SF12's increased neutralization breadth, potency, and resistance to Env mutation routes.					
31126879	7	51	theme	HIV-1	1141:1145	arg1	prevention					1147:1156	HIV-1 prevention	1141:1156	HIV-1 prevention	1141:1156	Effective bNAbs can therefore be raised against HIV-1 Env's silent face, suggesting their potential for HIV-1 prevention, therapy, and vaccine development.					
31126879	1	52	theme	HIV-1	136:140	arg1	Env					152:154	Env	152:154	Env	152:154	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	1	52	theme	HIV-1	136:140	arg1	envelope					142:149	HIV-1 envelope	136:149	HIV-1 envelope (Env)	136:155	Broadly neutralizing antibodies (bNAbs) against HIV-1 envelope (Env) inform vaccine design and are potential therapeutic agents.					
31126879	6	53	theme	humanized	948:956	arg1	mice					958:961	HIV-1-infected humanized mice	933:961	HIV-1-infected humanized mice	933:961	Administrating SF12 to HIV-1-infected humanized mice suppressed viremia and selected for viruses lacking the N448gp120 glycan.					
31126879	6	54	theme	N448gp120	1019:1027	arg1	glycan					1029:1034	the N448gp120 glycan	1015:1034	the N448gp120 glycan	1015:1034	Administrating SF12 to HIV-1-infected humanized mice suppressed viremia and selected for viruses lacking the N448gp120 glycan.					
29304471	2	0	gly	glycosylation	285:297	arg2	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	1	theme	E	433:433	arg1	dimers					435:440	E dimers	433:440	E dimers on mature viruses	433:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	1	2	theme	antigenic	163:171	arg1	structure					173:181	the antigenic structure	159:181	the antigenic structure of flaviviruses	159:197	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	3	3	theme	DENV	622:625	arg1	loop					634:637	the DENV glycan loop	618:637	the DENV glycan loop	618:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	2	4	theme	envelope	325:332	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	5	theme	N-linked	276:283	arg1	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	6	theme	glycosylation	285:297	arg1	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	6	7	theme	antigenicity	1142:1153	arg1	determinants					1098:1109	the determinants	1094:1109	the determinants of ZIKV E protein function and antigenicity	1094:1153	Our study provides insight into the determinants of ZIKV E protein function and antigenicity.					
29304471	2	8	theme	single	269:274	arg1	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	9	theme	virus	312:316	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	5	10	theme	E	898:898	arg1	glycan					908:913	the E protein glycan	894:913	the E protein glycan	894:913	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	4	11	theme	E	827:827	arg1	glycan					837:842	an E protein glycan	824:842	an E protein glycan	824:842	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	4	12	theme	neutralization	650:663	arg1	sensitivity					665:675	Increased neutralization sensitivity	640:675	Increased neutralization sensitivity	640:675	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	6	13	theme	ZIKV	1114:1117	arg1	function					1129:1136	ZIKV E protein function	1114:1136	ZIKV E protein function	1114:1136	Our study provides insight into the determinants of ZIKV E protein function and antigenicity.					
29304471	6	14	theme	function	1129:1136	arg1	determinants					1098:1109	the determinants	1094:1109	the determinants of ZIKV E protein function and antigenicity	1094:1153	Our study provides insight into the determinants of ZIKV E protein function and antigenicity.					
29304471	5	15	theme	lectin	967:972	arg1	DC-SIGNR					974:981	the lectin DC-SIGNR	963:981	the lectin DC-SIGNR	963:981	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	0	16	theme	Zika	4:7	arg1	loop					39:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	2	17	theme	Zika	307:310	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	1	18	theme	flaviviruses	186:197	arg1	structure					173:181	the antigenic structure	159:181	the antigenic structure of flaviviruses	159:197	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	2	19	contain	containing	254:263	arg2	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	19	contain	containing	254:263	arg1	loop					249:252	the loop	245:252	the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	245:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	4	20	theme	protein	829:835	arg1	glycan					837:842	an E protein glycan	824:842	an E protein glycan	824:842	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	4	21	theme	Increased	640:648	arg1	sensitivity					665:675	Increased neutralization sensitivity	640:675	Increased neutralization sensitivity	640:675	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	0	22	theme	envelope	15:22	arg1	loop					39:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	3	23	theme	DII-FL	500:505	arg1	antibodies					507:516	DII-FL antibodies	500:516	DII-FL antibodies	500:516	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	5	24	theme	prM	999:1001	arg1	glycan					1003:1008	the prM glycan	995:1008	the prM glycan of partially mature particles	995:1038	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	0	25	theme	virus	9:13	arg1	loop					39:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	2	26	link	N-linked	276:283	arg1	site					299:302	the single N-linked glycosylation site	265:302	the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins	265:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	27	from	dimers	435:440	arg1	viruses					452:458	mature viruses	445:458	mature viruses	445:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	1	28	theme	important	101:109	arg1	component					111:119	an important component	98:119	an important component of flavivirus immunity	98:142	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	1	28	theme	important	101:109	arg1	antibodies					83:92	antibodies	83:92	antibodies	83:92	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	2	29	theme	fusion	379:384	arg1	DII-FL					392:397	DII-FL	392:397	DII-FL	392:397	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	29	theme	fusion	379:384	arg1	loop					386:389	the DII fusion loop	371:389	the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses	371:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	0	30	theme	glycan	32:37	arg1	loop					39:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	4	31	theme	E	696:696	arg1	glycosylation					706:718	E protein glycosylation	696:718	E protein glycosylation	696:718	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	3	32	theme	glycan	627:632	arg1	loop					634:637	the DENV glycan loop	618:637	the DENV glycan loop	618:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	3	33	theme	increased	549:557	arg1	sensitivity					574:584	increased neutralization sensitivity	549:584	increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop	549:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	2	34	theme	DII	375:377	arg1	DII-FL					392:397	DII-FL	392:397	DII-FL	392:397	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	34	theme	DII	375:377	arg1	loop					386:389	the DII fusion loop	371:389	the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses	371:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	0	35	theme	protein	24:30	arg1	loop					39:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop	0:42	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	3	36	theme	neutralization	559:572	arg1	sensitivity					574:584	increased neutralization sensitivity	549:584	increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop	549:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	2	37	theme	E	415:415	arg1	proteins					417:424	neighboring E proteins	403:424	neighboring E proteins within E dimers on mature viruses	403:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	5	38	theme	ZIKV	871:874	arg1	particles					876:884	ZIKV particles	871:884	ZIKV particles lacking the E protein glycan	871:913	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	5	39	theme	particles	1030:1038	arg1	glycan					1003:1008	the prM glycan	995:1008	the prM glycan of partially mature particles	995:1038	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	4	40	theme	protein	698:704	arg1	glycosylation					706:718	E protein glycosylation	696:718	E protein glycosylation	696:718	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	2	41	theme	neighboring	403:413	arg1	proteins					417:424	neighboring E proteins	403:424	neighboring E proteins within E dimers on mature viruses	403:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	5	42	theme	mature	1023:1028	arg1	particles					1030:1038	partially mature particles	1013:1038	partially mature particles	1013:1038	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	2	43	theme	dengue	224:229	arg1	DENV					238:241	DENV	238:241	DENV	238:241	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	43	theme	dengue	224:229	arg1	virus					231:235	dengue virus	224:235	dengue virus (DENV)	224:242	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	44	theme	ZIKV	319:322	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	5	45	theme	protein	900:906	arg1	glycan					908:913	the E protein glycan	894:913	the E protein glycan	894:913	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	3	46	theme	ZIKV	589:592	arg1	particles					594:602	ZIKV particles	589:602	ZIKV particles incorporating the DENV glycan loop	589:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	1	47	theme	flavivirus	124:133	arg1	immunity					135:142	flavivirus immunity	124:142	flavivirus immunity	124:142	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	4	48	theme	protein	736:742	arg1	glycans					744:750	E protein glycans	734:750	E protein glycans	734:750	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	3	49	theme	particles	594:602	arg1	sensitivity					574:584	increased neutralization sensitivity	549:584	increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop	549:637	Although ZIKV is poorly neutralized by DII-FL antibodies, we demonstrated significantly increased neutralization sensitivity of ZIKV particles incorporating the DENV glycan loop.					
29304471	4	50	theme	E	734:734	arg1	glycans					744:750	E protein glycans	734:750	E protein glycans	734:750	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	1	51	theme	immunity	135:142	arg1	component					111:119	an important component	98:119	an important component of flavivirus immunity	98:142	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	1	51	theme	immunity	135:142	arg1	antibodies					83:92	antibodies	83:92	antibodies	83:92	Because antibodies are an important component of flavivirus immunity, understanding the antigenic structure of flaviviruses is critical.					
29304471	6	52	theme	E	1119:1119	arg1	function					1129:1136	ZIKV E protein function	1114:1136	ZIKV E protein function	1114:1136	Our study provides insight into the determinants of ZIKV E protein function and antigenicity.					
29304471	4	53	theme	glycosylation	706:718	arg1	independent					681:691	independent	681:691	independent	681:691	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	2	54	theme	mature	445:450	arg1	viruses					452:458	mature viruses	445:458	mature viruses	445:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	4	55	theme	loop	794:797	arg1	chimeras					799:806	ZIKV loop chimeras	789:806	ZIKV loop chimeras with or without an E protein glycan	789:842	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	5	56	theme	Raji	941:944	arg1	cells					946:950	Raji cells	941:950	Raji cells expressing the lectin DC-SIGNR	941:981	ZIKV particles lacking the E protein glycan were capable of infecting Raji cells expressing the lectin DC-SIGNR, suggesting the prM glycan of partially mature particles can facilitate entry.					
29304471	0	57	theme	virion	54:59	arg1	antigenicity					61:72	virion antigenicity	54:72	virion antigenicity	54:72	The Zika virus envelope protein glycan loop regulates virion antigenicity.					
29304471	6	58	theme	protein	1121:1127	arg1	function					1129:1136	ZIKV E protein function	1114:1136	ZIKV E protein function	1114:1136	Our study provides insight into the determinants of ZIKV E protein function and antigenicity.					
29304471	4	59	theme	ZIKV	789:792	arg1	chimeras					799:806	ZIKV loop chimeras	789:806	ZIKV loop chimeras with or without an E protein glycan	789:842	Increased neutralization sensitivity was independent of E protein glycosylation: ZIKV lacking E protein glycans remained poorly neutralized, whereas ZIKV loop chimeras with or without an E protein glycan were potently neutralized.					
29304471	2	60	from	loop	386:389	arg1	proteins					417:424	neighboring E proteins	403:424	neighboring E proteins within E dimers on mature viruses	403:458	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	61	theme	E	335:335	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29304471	2	62	from	site	299:302	arg1	proteins					338:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Zika virus (ZIKV) envelope (E) proteins	307:345	Compared to dengue virus (DENV), the loop containing the single N-linked glycosylation site on Zika virus (ZIKV) envelope (E) proteins extends further towards the DII fusion loop (DII-FL) on neighboring E proteins within E dimers on mature viruses.					
29502007	2	0	theme	recent	335:340	arg1	emergence					342:350	the recent emergence	331:350	the recent emergence of biosimilar medicines	331:374	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	8	1	theme	monitoring	1777:1786	arg1	profiles					1802:1809	monitoring glycosylation profiles	1777:1809	monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products	1777:1897	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	8	2	theme	unexpected	1938:1947	arg1	glycans					1949:1955	unusual or unexpected glycans	1927:1955	unusual or unexpected glycans	1927:1955	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	7	3	theme	observed	1554:1561	arg1	differences					1563:1573	the observed differences	1550:1573	the observed differences in glycosylation profile	1550:1598	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	8	4	theme	biosimilars	1725:1735	arg1	emergence					1712:1720	the continued emergence	1698:1720	the continued emergence of biosimilars	1698:1735	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	1	5	gly	glycoprotein	173:184	arg1	glycoprotein					173:184	a glycoprotein hormone	171:192	a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy	171:299	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	8	6	theme	profiles	1802:1809	arg1	importance					1763:1772	the importance	1759:1772	the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products	1759:1897	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	8	6	theme	profiles	1802:1809	arg1	presence					1915:1922	the presence	1911:1922	the presence of unusual or unexpected glycans	1911:1955	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	1	7	theme	chronic	262:268	arg1	disease					277:283	chronic kidney disease	262:283	chronic kidney disease	262:283	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	1	8	theme	Recombinant	125:135	arg1	hormone					186:192	a glycoprotein hormone	171:192	a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy	171:299	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	1	8	theme	Recombinant	125:135	arg1	rhEPO					161:165	rhEPO	161:165	rhEPO	161:165	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	1	8	theme	Recombinant	125:135	arg1	erythropoietin					145:158	Recombinant, human, erythropoietin	125:158	erythropoietin	145:158	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	8	9	theme	biological	1814:1823	arg1	medicines					1825:1833	biological medicines	1814:1833	biological medicines	1814:1833	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	2	10	theme	biosimilar	355:364	arg1	medicines					366:374	biosimilar medicines	355:374	biosimilar medicines	355:374	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	4	11	theme	isoform	857:863	arg1	distribution					865:876	isoform distribution	857:876	isoform distribution via capillary electrophoresis (CE)	857:911	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	12	attach	presence	1324:1331	arg2	levels					1351:1356	unusually high levels	1336:1356	unusually high levels of suspected penta- and hexa-anionic N-glycans	1336:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	6	12	attach	presence	1324:1331	arg1	samples					1416:1422	several samples	1408:1422	several samples	1408:1422	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	0	13	from	manufacturers	91:103	arg1	preparations					73:84	preparations	73:84	preparations	73:84	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	0	13	from	manufacturers	91:103	arg1	Japan					118:122	Japan	118:122	Japan	118:122	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	0	13	from	manufacturers	91:103	arg1	China					108:112	China	108:112	China	108:112	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	3	14	theme	Due	425:427	arg1	preparations					541:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations	425:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations	425:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	7	15	contain	have	1617:1620	arg1	differences					1563:1573	the observed differences	1550:1573	the observed differences in glycosylation profile	1550:1598	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	7	15	contain	have	1617:1620	arg2	influence					1636:1644	a significant influence	1622:1644	a significant influence	1622:1644	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	7	16	from	differences	1563:1573	arg1	profile					1592:1598	glycosylation profile	1578:1598	glycosylation profile	1578:1598	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	6	17	theme	hexa-anionic	1382:1393	arg1	N-glycans					1395:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	8	18	from	importance	1763:1772	arg1	medicines					1825:1833	biological medicines	1814:1833	biological medicines	1814:1833	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	6	19	theme	penta-	1371:1376	arg1	N-glycans					1395:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	5	20	theme	glycosylation	1098:1110	arg1	occurrence					1144:1153	the varying occurrence	1132:1153	the varying occurrence of sialic acid O-acetylation	1132:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	20	theme	glycosylation	1098:1110	arg1	profiles					1112:1119	glycosylation profiles	1098:1119	glycosylation profiles	1098:1119	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	20	theme	glycosylation	1098:1110	arg1	extension					1185:1193	extension	1185:1193	extension of N-glycan antennae with N-acetyllactosamine units	1185:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	20	theme	glycosylation	1098:1110	arg1	distribution					1256:1267	the distribution	1252:1267	the distribution of sialic acids across multi-antennary structures	1252:1317	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	21	theme	anion-exchange/hydrophilic	969:994	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	5	22	theme	O-acetylation	1170:1182	arg1	occurrence					1144:1153	the varying occurrence	1132:1153	the varying occurrence of sialic acid O-acetylation	1132:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	22	theme	O-acetylation	1170:1182	arg1	extension					1185:1193	extension	1185:1193	extension of N-glycan antennae with N-acetyllactosamine units	1185:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	22	theme	O-acetylation	1170:1182	arg1	distribution					1256:1267	the distribution	1252:1267	the distribution of sialic acids across multi-antennary structures	1252:1317	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	23	theme	chromatography-mass	1008:1026	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	8	24	from	presence	1915:1922	arg1	medicines					1825:1833	biological medicines	1814:1833	biological medicines	1814:1833	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	6	25	theme	isoform	1458:1464	arg1	acidity					1466:1472	elevated rhEPO isoform acidity	1443:1472	elevated rhEPO isoform acidity	1443:1472	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	6	26	theme	high	1346:1349	arg1	levels					1351:1356	unusually high levels	1336:1356	unusually high levels of suspected penta- and hexa-anionic N-glycans	1336:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	7	27	theme	biological	1651:1660	arg1	activity					1662:1669	biological activity	1651:1669	biological activity in mice	1651:1677	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	4	28	theme	biological	744:753	arg1	activity					755:762	in vivo biological activity	736:762	in vivo biological activity	736:762	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	29	theme	elevated	1443:1450	arg1	acidity					1466:1472	elevated rhEPO isoform acidity	1443:1472	elevated rhEPO isoform acidity	1443:1472	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	1	30	dep	Recombinant	125:135	arg1	human					138:142	human	138:142	human	138:142	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	2	31	theme	numerous	382:389	arg1	products					391:398	numerous products	382:398	numerous products now available worldwide	382:422	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	5	32	theme	acids	1279:1283	arg1	occurrence					1144:1153	the varying occurrence	1132:1153	the varying occurrence of sialic acid O-acetylation	1132:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	32	theme	acids	1279:1283	arg1	extension					1185:1193	extension	1185:1193	extension of N-glycan antennae with N-acetyllactosamine units	1185:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	32	theme	acids	1279:1283	arg1	distribution					1256:1267	the distribution	1252:1267	the distribution of sialic acids across multi-antennary structures	1252:1317	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	33	theme	sialic	1158:1163	arg1	O-acetylation					1170:1182	sialic acid O-acetylation	1158:1182	sialic acid O-acetylation	1158:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	0	34	theme	Comprehensive	0:12	arg1	analysis					21:28	Comprehensive glycan analysis	0:28	Comprehensive glycan analysis of twelve recombinant human erythropoietin	0:71	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	1	35	theme	kidney	270:275	arg1	disease					277:283	chronic kidney disease	262:283	chronic kidney disease	262:283	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	8	36	theme	unusual	1927:1933	arg1	glycans					1949:1955	unusual or unexpected glycans	1927:1955	unusual or unexpected glycans	1927:1955	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	4	37	theme	in	736:737	arg1	activity					755:762	in vivo biological activity	736:762	in vivo biological activity	736:762	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	38	theme	elevated	1506:1513	arg1	bioactivities					1523:1535	slightly elevated in vivo bioactivities	1497:1535	slightly elevated in vivo bioactivities	1497:1535	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	2	39	with	forefront	318:326	arg1	worldwide					414:422	numerous products now available worldwide	382:422	numerous products now available worldwide	382:422	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	8	40	theme	glycans	1949:1955	arg1	importance					1763:1772	the importance	1759:1772	the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products	1759:1897	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	8	40	theme	glycans	1949:1955	arg1	presence					1915:1922	the presence	1911:1922	the presence of unusual or unexpected glycans	1911:1955	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	3	41	theme	complex	436:442	arg1	profile					458:464	its complex glycosylation profile	432:464	its complex glycosylation profile	432:464	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	5	42	theme	N-glycan	1198:1205	arg1	antennae					1207:1214	N-glycan antennae	1198:1214	N-glycan antennae	1198:1214	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	3	43	dep	Due	425:427	arg1	therapeutic					523:533	therapeutic	523:533	therapeutic	523:533	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	4	44	theme	acid	829:832	arg1	determination					842:854	sialic acid content determination	822:854	sialic acid content determination	822:854	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	0	45	theme	human	52:56	arg1	erythropoietin					58:71	twelve recombinant human erythropoietin	33:71	twelve recombinant human erythropoietin	33:71	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	6	46	dep	in	1515:1516	arg1	vivo					1518:1521	vivo	1518:1521	vivo	1518:1521	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	4	47	from	one	712:714	arg1	Japan					719:723	Japan	719:723	Japan	719:723	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	47	from	one	712:714	arg1	preparations					660:671	twelve rhEPO preparations	647:671	twelve rhEPO preparations	647:671	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	7	48	theme	glycosylation	1578:1590	arg1	profile					1592:1598	glycosylation profile	1578:1598	glycosylation profile	1578:1598	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	2	49	theme	products	391:398	arg1	worldwide					414:422	numerous products now available worldwide	382:422	numerous products now available worldwide	382:422	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	3	50	theme	biological	502:511	arg1	activity					513:520	biological activity	502:520	biological activity	502:520	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	6	51	theme	suspected	1361:1369	arg1	N-glycans					1395:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	suspected penta- and hexa-anionic N-glycans	1361:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	2	52	theme	emergence	342:350	arg1	forefront					318:326	the forefront	314:326	the forefront of the recent emergence of biosimilar medicines	314:374	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	4	53	from	manufacturers	685:697	arg1	China					702:706	China	702:706	China	702:706	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	53	from	manufacturers	685:697	arg1	preparations					660:671	twelve rhEPO preparations	647:671	twelve rhEPO preparations	647:671	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	54	from	presence	1324:1331	arg1	samples					1416:1422	several samples	1408:1422	several samples	1408:1422	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	8	55	theme	continued	1702:1710	arg1	emergence					1712:1720	the continued emergence	1698:1720	the continued emergence of biosimilars	1698:1735	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	6	56	theme	levels	1351:1356	arg1	consistent					1427:1436	consistent	1427:1436	consistent	1427:1436	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	6	56	theme	levels	1351:1356	arg1	presence					1324:1331	The presence	1320:1331	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples	1320:1422	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	8	57	from	profiles	1802:1809	arg1	medicines					1825:1833	biological medicines	1814:1833	biological medicines	1814:1833	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	3	58	theme	rhEPO	535:539	arg1	preparations					541:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations	425:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations	425:552	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	5	59	theme	sialic	1272:1277	arg1	acids					1279:1283	sialic acids	1272:1283	sialic acids	1272:1283	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	60	theme	capillary	882:890	arg1	CE					909:910	CE	909:910	CE	909:910	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	60	theme	capillary	882:890	arg1	electrophoresis					892:906	capillary electrophoresis	882:906	capillary electrophoresis (CE)	882:911	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	8	61	theme	glycosylation	1788:1800	arg1	profiles					1802:1809	monitoring glycosylation profiles	1777:1809	monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products	1777:1897	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	4	62	theme	content	834:840	arg1	determination					842:854	sialic acid content determination	822:854	sialic acid content determination	822:854	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	1	63	theme	glycoprotein	173:184	arg1	hormone					186:192	a glycoprotein hormone	171:192	a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy	171:299	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	1	63	theme	glycoprotein	173:184	arg1	erythropoietin					145:158	Recombinant, human, erythropoietin	125:158	erythropoietin	145:158	Recombinant, human, erythropoietin (rhEPO) is a glycoprotein hormone which is prescribed throughout the world to treat anaemia caused by chronic kidney disease or chemotherapy.					
29502007	4	64	theme	rhEPO	654:658	arg1	preparations					660:671	twelve rhEPO preparations	647:671	twelve rhEPO preparations	647:671	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	65	theme	O-glycan	914:921	arg1	profiling					923:931	O-glycan profiling	914:931	O-glycan profiling	914:931	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	66	theme	several	1408:1414	arg1	samples					1416:1422	several samples	1408:1422	several samples	1408:1422	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	6	67	theme	N-glycans	1395:1403	arg1	levels					1351:1356	unusually high levels	1336:1356	unusually high levels of suspected penta- and hexa-anionic N-glycans	1336:1403	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	5	68	theme	varying	1136:1142	arg1	occurrence					1144:1153	the varying occurrence	1132:1153	the varying occurrence of sialic acid O-acetylation	1132:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	69	dep	in	736:737	arg1	vivo					739:742	vivo	739:742	vivo	739:742	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	5	70	with	occurrence	1144:1153	arg1	units					1241:1245	N-acetyllactosamine units	1221:1245	N-acetyllactosamine units	1221:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	6	71	with	consistent	1427:1436	arg1	acidity					1466:1472	elevated rhEPO isoform acidity	1443:1472	elevated rhEPO isoform acidity	1443:1472	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	4	72	theme	novel	963:967	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	5	73	with	extension	1185:1193	arg1	units					1241:1245	N-acetyllactosamine units	1221:1245	N-acetyllactosamine units	1221:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	74	theme	interaction	996:1006	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	75	with	relationship	782:793	arg1	glycosylation					800:812	glycosylation	800:812	glycosylation	800:812	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	6	76	theme	rhEPO	1452:1456	arg1	acidity					1466:1472	elevated rhEPO isoform acidity	1443:1472	elevated rhEPO isoform acidity	1443:1472	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	7	77	theme	significant	1624:1634	arg1	influence					1636:1644	a significant influence	1622:1644	a significant influence	1622:1644	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	4	78	theme	N-glycan	938:945	arg1	mapping					947:953	N-glycan mapping	938:953	N-glycan mapping	938:953	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	3	79	contain	has	473:475	arg1	profile					458:464	its complex glycosylation profile	432:464	its complex glycosylation profile	432:464	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	3	79	contain	has	473:475	arg2	influence					487:495	a crucial influence	477:495	a crucial influence	477:495	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	0	80	theme	glycan	14:19	arg1	analysis					21:28	Comprehensive glycan analysis	0:28	Comprehensive glycan analysis of twelve recombinant human erythropoietin	0:71	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	5	81	theme	acid	1165:1168	arg1	O-acetylation					1170:1182	sialic acid O-acetylation	1158:1182	sialic acid O-acetylation	1158:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	2	82	theme	medicines	366:374	arg1	emergence					342:350	the recent emergence	331:350	the recent emergence of biosimilar medicines	331:374	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	6	83	theme	in	1515:1516	arg1	bioactivities					1523:1535	slightly elevated in vivo bioactivities	1497:1535	slightly elevated in vivo bioactivities	1497:1535	The presence of unusually high levels of suspected penta- and hexa-anionic N-glycans in several samples is consistent with elevated rhEPO isoform acidity, which is reflected by slightly elevated in vivo bioactivities.					
29502007	2	84	theme	available	404:412	arg1	products					391:398	numerous products	382:398	numerous products now available worldwide	382:422	rhEPO is at the forefront of the recent emergence of biosimilar medicines, with numerous products now available worldwide.					
29502007	0	85	theme	recombinant	40:50	arg1	erythropoietin					58:71	twelve recombinant human erythropoietin	33:71	twelve recombinant human erythropoietin	33:71	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	4	86	theme	spectrometry	1028:1039	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	3	87	theme	glycosylation	444:456	arg1	profile					458:464	its complex glycosylation profile	432:464	its complex glycosylation profile	432:464	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	0	88	theme	erythropoietin	58:71	arg1	analysis					21:28	Comprehensive glycan analysis	0:28	Comprehensive glycan analysis of twelve recombinant human erythropoietin	0:71	Comprehensive glycan analysis of twelve recombinant human erythropoietin preparations from manufacturers in China and Japan.					
29502007	5	89	theme	antennae	1207:1214	arg1	occurrence					1144:1153	the varying occurrence	1132:1153	the varying occurrence of sialic acid O-acetylation	1132:1182	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	89	theme	antennae	1207:1214	arg1	extension					1185:1193	extension	1185:1193	extension of N-glycan antennae with N-acetyllactosamine units	1185:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	5	89	theme	antennae	1207:1214	arg1	distribution					1256:1267	the distribution	1252:1267	the distribution of sialic acids across multi-antennary structures	1252:1317	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	7	90	from	activity	1662:1669	arg1	mice					1674:1677	mice	1674:1677	mice	1674:1677	This aside, the observed differences in glycosylation profile do not appear to have a significant influence upon biological activity in mice.					
29502007	4	91	theme	AEX/HILIC-MS	1042:1053	arg1	approach					1056:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach	961:1063	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	92	theme	sialic	822:827	arg1	determination					842:854	sialic acid content determination	822:854	sialic acid content determination	822:854	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	5	93	with	distribution	1256:1267	arg1	units					1241:1245	N-acetyllactosamine units	1221:1245	N-acetyllactosamine units	1221:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	3	94	theme	crucial	479:485	arg1	influence					487:495	a crucial influence	477:495	a crucial influence	477:495	Due to its complex glycosylation profile, which has a crucial influence upon biological activity, therapeutic rhEPO preparations must be closely monitored to ensure consistency, safety and efficacy.					
29502007	5	95	theme	N-acetyllactosamine	1221:1239	arg1	units					1241:1245	N-acetyllactosamine units	1221:1245	N-acetyllactosamine units	1221:1245	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	4	96	dep	preparations	660:671	arg1	measuring					726:734	measuring	726:734	measuring in vivo biological activity	726:762	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	4	96	dep	preparations	660:671	arg1	exploring					768:776	exploring	768:776	exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping	768:953	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29502007	5	97	theme	multi-antennary	1292:1306	arg1	structures					1308:1317	multi-antennary structures	1292:1317	multi-antennary structures	1292:1317	We observed differences between glycosylation profiles, including the varying occurrence of sialic acid O-acetylation, extension of N-glycan antennae with N-acetyllactosamine units, and the distribution of sialic acids across multi-antennary structures.					
29502007	8	98	from	medicines	1825:1833	arg1	importance					1763:1772	the importance	1759:1772	the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products	1759:1897	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	8	98	from	medicines	1825:1833	arg1	presence					1915:1922	the presence	1911:1922	the presence of unusual or unexpected glycans	1911:1955	Nonetheless, with the continued emergence of biosimilars, the study highlights the importance of monitoring glycosylation profiles in biological medicines, in order to detect and account for divergence between products, as well as the presence of unusual or unexpected glycans.					
29502007	4	99	gly	glycosylation	800:812	arg1	relationship					782:793	its relationship	778:793	its relationship with glycosylation	778:812	Here, we have compared twelve rhEPO preparations from eleven manufacturers in China and one in Japan, measuring in vivo biological activity and exploring its relationship with glycosylation through sialic acid content determination, isoform distribution via capillary electrophoresis (CE), O-glycan profiling, and N-glycan mapping using a novel anion-exchange/hydrophilic interaction chromatography-mass spectrometry (AEX/HILIC-MS) approach.					
29659495	8	0	theme	Agrobacterium	1290:1302	arg1	ratio					1281:1285	The mass ratio	1272:1285	The mass ratio of Agrobacterium to plant cells used	1272:1322	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	6	1	from	&beta	999:1003	arg1	&alpha					1033:1038	&alpha	1033:1038	&alpha	1033:1038	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	1	from	&beta	999:1003	arg1	-1,2-xylosyltransferase					1005:1027	-1,2-xylosyltransferase	1005:1027	-1,2-xylosyltransferase	1005:1027	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	1	from	&beta	999:1003	arg1	cultures					985:992	cell suspension cultures	969:992	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	2	attach	presence	887:894	arg1	product					929:935	the product	925:935	the product	925:935	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	2	attach	presence	887:894	arg2	glycans					914:920	plant-specific glycans	899:920	plant-specific glycans	899:920	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	4	3	theme	protein	639:645	arg1	safety					601:606	safety	601:606	safety	601:606	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	4	3	theme	protein	639:645	arg1	efficacy					591:598	efficacy	591:598	efficacy	591:598	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	4	3	theme	protein	639:645	arg1	stability					612:620	stability	612:620	stability	612:620	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	2	4	theme	Agrobacterium	313:325	arg1	tumefaciens					327:337	Agrobacterium tumefaciens	313:337	Agrobacterium tumefaciens	313:337	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	2	4	theme	Agrobacterium	313:325	arg1	vector					370:375	a gene delivery vector	354:375	a gene delivery vector for transient expression	354:400	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	6	5	theme	cell	969:972	arg1	&alpha					1033:1038	&alpha	1033:1038	&alpha	1033:1038	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	5	theme	cell	969:972	arg1	-1,2-xylosyltransferase					1005:1027	-1,2-xylosyltransferase	1005:1027	-1,2-xylosyltransferase	1005:1027	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	5	theme	cell	969:972	arg1	cultures					985:992	cell suspension cultures	969:992	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	4	6	theme	plant-based	706:716	arg1	systems					718:724	plant-based systems	706:724	plant-based systems	706:724	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	10	7	theme	cell	1755:1758	arg1	host					1768:1771	a glycoengineered plant cell culture host	1731:1771	a glycoengineered plant cell culture host	1731:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	4	8	from	distributions	689:701	arg1	systems					718:724	plant-based systems	706:724	plant-based systems	706:724	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	1	9	theme	emergency	222:230	arg1	situations					232:241	emergency situations	222:241	emergency situations in which rapid production of novel therapeutics is needed	222:299	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	6	10	theme	Nicotiana	1074:1082	arg1	benthamiana					1084:1094	-1,3-fucosyltransferase knockdown Nicotiana benthamiana	1040:1094	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	8	11	theme	protein	1356:1362	arg1	levels					1375:1380	recombinant protein expression levels	1344:1380	recombinant protein expression levels	1344:1380	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	6	12	theme	-1,3-fucosyltransferase	1040:1062	arg1	benthamiana					1084:1094	-1,3-fucosyltransferase knockdown Nicotiana benthamiana	1040:1094	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	7	13	theme	cell	1185:1188	arg1	cultures					1201:1208	these glycoengineered plant cell suspension cultures	1157:1208	these glycoengineered plant cell suspension cultures	1157:1208	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	1	14	theme	rapid	252:256	arg1	production					258:267	rapid production	252:267	rapid production of novel therapeutics	252:289	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	9	15	from	analysis	1399:1406	arg1	protein					1436:1442	the anthrax decoy fusion protein	1411:1442	the anthrax decoy fusion protein produced in glycoengineered N. benthamiana	1411:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	7	16	theme	glycoengineered	1163:1177	arg1	cultures					1201:1208	these glycoengineered plant cell suspension cultures	1157:1208	these glycoengineered plant cell suspension cultures	1157:1208	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	7	17	theme	fusion	1114:1119	arg1	protein					1121:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	9	18	theme	decoy	1423:1427	arg1	protein					1436:1442	the anthrax decoy fusion protein	1411:1442	the anthrax decoy fusion protein produced in glycoengineered N. benthamiana	1411:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	7	19	theme	anthrax	1100:1106	arg1	protein					1121:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	5	20	theme	glycoengineered	825:839	arg1	cultures					863:870	glycoengineered plant cell suspension cultures	825:870	glycoengineered plant cell suspension cultures	825:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	1	21	theme	stable	178:183	arg1	systems					196:202	stable transgenic systems	178:202	stable transgenic systems	178:202	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	10	22	theme	rapid	1621:1625	arg1	process					1640:1646	a simple, rapid and scalable process	1611:1646	a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1611:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	5	23	theme	cell	847:850	arg1	cultures					863:870	glycoengineered plant cell suspension cultures	825:870	glycoengineered plant cell suspension cultures	825:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	3	24	located	found	517:521	arg2	patterns					504:511	different glycosylation patterns	480:511	different glycosylation patterns	480:511	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	3	24	located	found	517:521	arg1	plant					526:530	plant	526:530	plant	526:530	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	3	24	located	found	517:521	arg1	proteins					546:553	mammalian proteins	536:553	mammalian proteins	536:553	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	10	25	theme	simple	1613:1618	arg1	process					1640:1646	a simple, rapid and scalable process	1611:1646	a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1611:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	5	26	theme	Agrobacterium-mediated	778:799	arg1	expression					811:820	Agrobacterium-mediated transient expression	778:820	Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures	778:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	9	27	theme	N.	1472:1473	arg1	benthamiana					1475:1485	glycoengineered N. benthamiana	1456:1485	glycoengineered N. benthamiana	1456:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	0	28	theme	Glycoengineered	44:58	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	10	29	theme	scalable	1631:1638	arg1	process					1640:1646	a simple, rapid and scalable process	1611:1646	a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1611:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	3	30	theme	therapeutics	459:470	arg1	production					439:448	plant-based production	427:448	plant-based production of human therapeutics	427:470	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	0	31	theme	Transient	0:8	arg1	Production					30:39	Transient Recombinant Protein Production	0:39	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.	0:105	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	9	32	theme	dramatic	1496:1503	arg1	reduction					1505:1513	a dramatic reduction	1494:1513	a dramatic reduction in plant-specific N-glycans	1494:1541	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	9	33	from	reduction	1505:1513	arg1	N-glycans					1533:1541	plant-specific N-glycans	1518:1541	plant-specific N-glycans	1518:1541	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	1	34	theme	recombinant	117:127	arg1	alternative					163:173	a promising alternative	151:173	a promising alternative	151:173	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	1	34	theme	recombinant	117:127	arg1	production					137:146	Transient recombinant protein production	107:146	Transient recombinant protein production	107:146	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	6	35	from	presence	887:894	arg1	product					929:935	the product	925:935	the product	925:935	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	10	36	theme	recombinant	1676:1686	arg1	proteins					1688:1695	recombinant proteins	1676:1695	recombinant proteins without plant-specific glycans	1676:1726	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	0	37	theme	Protein	22:28	arg1	Production					30:39	Transient Recombinant Protein Production	0:39	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.	0:105	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	3	38	theme	glycosylation	490:502	arg1	patterns					504:511	different glycosylation patterns	480:511	different glycosylation patterns	480:511	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	3	39	theme	plant-based	427:437	arg1	production					439:448	plant-based production	427:448	plant-based production of human therapeutics	427:470	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	0	40	theme	Cell	82:85	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	0	41	theme	Nicotiana	60:68	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	7	42	with	co-culture	1218:1227	arg1	Agrobacterium					1257:1269	genetically engineered Agrobacterium	1234:1269	genetically engineered Agrobacterium	1234:1269	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	2	43	theme	gene	356:359	arg1	tumefaciens					327:337	Agrobacterium tumefaciens	313:337	Agrobacterium tumefaciens	313:337	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	2	43	theme	gene	356:359	arg1	vector					370:375	a gene delivery vector	354:375	a gene delivery vector for transient expression	354:400	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	6	44	theme	glycans	914:920	arg1	presence					887:894	the presence	883:894	the presence of plant-specific glycans on the product	883:935	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	2	45	used	used	346:349	arg2	vector					370:375	a gene delivery vector	354:375	a gene delivery vector for transient expression	354:400	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	2	45	used	used	346:349	arg2	tumefaciens					327:337	Agrobacterium tumefaciens	313:337	Agrobacterium tumefaciens	313:337	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	10	46	theme	plant	1749:1753	arg1	host					1768:1771	a glycoengineered plant cell culture host	1731:1771	a glycoengineered plant cell culture host	1731:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	1	47	theme	novel	272:276	arg1	therapeutics					278:289	novel therapeutics	272:289	novel therapeutics	272:289	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	8	48	theme	mass	1276:1279	arg1	ratio					1281:1285	The mass ratio	1272:1285	The mass ratio of Agrobacterium to plant cells used	1272:1322	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	5	49	theme	plant	841:845	arg1	cultures					863:870	glycoengineered plant cell suspension cultures	825:870	glycoengineered plant cell suspension cultures	825:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	2	50	theme	transient	381:389	arg1	expression					391:400	transient expression	381:400	transient expression	381:400	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	6	51	dep	cultures	985:992	arg1	&alpha					1033:1038	&alpha	1033:1038	&alpha	1033:1038	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	51	dep	cultures	985:992	arg1	-1,2-xylosyltransferase					1005:1027	-1,2-xylosyltransferase	1005:1027	-1,2-xylosyltransferase	1005:1027	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	51	dep	cultures	985:992	arg1	cultures					985:992	cell suspension cultures	969:992	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	51	dep	cultures	985:992	arg1	benthamiana					1084:1094	-1,3-fucosyltransferase knockdown Nicotiana benthamiana	1040:1094	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	7	52	theme	engineered	1246:1255	arg1	Agrobacterium					1257:1269	genetically engineered Agrobacterium	1234:1269	genetically engineered Agrobacterium	1234:1269	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	6	53	theme	suspension	974:983	arg1	&alpha					1033:1038	&alpha	1033:1038	&alpha	1033:1038	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	53	theme	suspension	974:983	arg1	-1,2-xylosyltransferase					1005:1027	-1,2-xylosyltransferase	1005:1027	-1,2-xylosyltransferase	1005:1027	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	6	53	theme	suspension	974:983	arg1	cultures					985:992	cell suspension cultures	969:992	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	4	54	theme	therapeutic	627:637	arg1	protein					639:645	a therapeutic protein	625:645	a therapeutic protein	625:645	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	10	55	theme	culture	1760:1766	arg1	host					1768:1771	a glycoengineered plant cell culture host	1731:1771	a glycoengineered plant cell culture host	1731:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	4	56	gly	glycosylation	562:574	arg1	protein					639:645	a therapeutic protein	625:645	a therapeutic protein	625:645	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	7	57	theme	suspension	1190:1199	arg1	cultures					1201:1208	these glycoengineered plant cell suspension cultures	1157:1208	these glycoengineered plant cell suspension cultures	1157:1208	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	6	58	theme	knockdown	1064:1072	arg1	benthamiana					1084:1094	-1,3-fucosyltransferase knockdown Nicotiana benthamiana	1040:1094	cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana	969:1094	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	4	59	theme	glycan	667:672	arg1	structures					674:683	control glycan structures	659:683	control glycan structures	659:683	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	2	60	theme	delivery	361:368	arg1	tumefaciens					327:337	Agrobacterium tumefaciens	313:337	Agrobacterium tumefaciens	313:337	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	2	60	theme	delivery	361:368	arg1	vector					370:375	a gene delivery vector	354:375	a gene delivery vector for transient expression	354:400	In plants, Agrobacterium tumefaciens can be used as a gene delivery vector for transient expression.					
29659495	7	61	theme	plant	1179:1183	arg1	cultures					1201:1208	these glycoengineered plant cell suspension cultures	1157:1208	these glycoengineered plant cell suspension cultures	1157:1208	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	9	62	theme	N-glycosylation	1383:1397	arg1	analysis					1399:1406	N-glycosylation analysis	1383:1406	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana	1383:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	8	63	theme	recombinant	1344:1354	arg1	levels					1375:1380	recombinant protein expression levels	1344:1380	recombinant protein expression levels	1344:1380	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	8	64	theme	expression	1364:1373	arg1	levels					1375:1380	recombinant protein expression levels	1344:1380	recombinant protein expression levels	1344:1380	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	9	65	theme	anthrax	1415:1421	arg1	protein					1436:1442	the anthrax decoy fusion protein	1411:1442	the anthrax decoy fusion protein produced in glycoengineered N. benthamiana	1411:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	1	66	theme	promising	153:161	arg1	alternative					163:173	a promising alternative	151:173	a promising alternative	151:173	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	1	66	theme	promising	153:161	arg1	production					137:146	Transient recombinant protein production	107:146	Transient recombinant protein production	107:146	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	10	67	from	production	1662:1671	arg1	host					1768:1771	a glycoengineered plant cell culture host	1731:1771	a glycoengineered plant cell culture host	1731:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	0	68	theme	Suspension	87:96	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	9	69	theme	fusion	1429:1434	arg1	protein					1436:1442	the anthrax decoy fusion protein	1411:1442	the anthrax decoy fusion protein produced in glycoengineered N. benthamiana	1411:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	7	70	theme	decoy	1108:1112	arg1	protein					1121:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein	1097:1127	An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered Agrobacterium.					
29659495	0	71	from	Production	30:39	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	4	72	from	methods	648:654	arg1	systems					718:724	plant-based systems	706:724	plant-based systems	706:724	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	1	73	theme	transgenic	185:194	arg1	systems					196:202	stable transgenic systems	178:202	stable transgenic systems	178:202	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	9	74	theme	glycoengineered	1456:1470	arg1	benthamiana					1475:1485	glycoengineered N. benthamiana	1456:1485	glycoengineered N. benthamiana	1456:1485	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	5	75	theme	transient	801:809	arg1	expression					811:820	Agrobacterium-mediated transient expression	778:820	Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures	778:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	10	76	theme	process	1640:1646	arg1	feasibility					1596:1606	the feasibility	1592:1606	the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1592:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	3	77	theme	different	480:488	arg1	patterns					504:511	different glycosylation patterns	480:511	different glycosylation patterns	480:511	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	0	78	theme	Recombinant	10:20	arg1	Production					30:39	Transient Recombinant Protein Production	0:39	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.	0:105	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	1	79	theme	Transient	107:115	arg1	alternative					163:173	a promising alternative	151:173	a promising alternative	151:173	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	1	79	theme	Transient	107:115	arg1	production					137:146	Transient recombinant protein production	107:146	Transient recombinant protein production	107:146	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	5	80	from	expression	811:820	arg1	cultures					863:870	glycoengineered plant cell suspension cultures	825:870	glycoengineered plant cell suspension cultures	825:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	1	81	theme	protein	129:135	arg1	alternative					163:173	a promising alternative	151:173	a promising alternative	151:173	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	1	81	theme	protein	129:135	arg1	production					137:146	Transient recombinant protein production	107:146	Transient recombinant protein production	107:146	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
29659495	3	82	theme	potential	405:413	arg1	barrier					415:421	A potential barrier	403:421	A potential barrier for plant-based production of human therapeutics	403:470	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	5	83	theme	suspension	852:861	arg1	cultures					863:870	glycoengineered plant cell suspension cultures	825:870	glycoengineered plant cell suspension cultures	825:870	In these studies, we performed Agrobacterium-mediated transient expression in glycoengineered plant cell suspension cultures.					
29659495	9	84	theme	plant-specific	1518:1531	arg1	N-glycans					1533:1541	plant-specific N-glycans	1518:1541	plant-specific N-glycans	1518:1541	N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered N. benthamiana showed a dramatic reduction in plant-specific N-glycans.					
29659495	3	85	theme	human	453:457	arg1	therapeutics					459:470	human therapeutics	453:470	human therapeutics	453:470	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	10	86	theme	transient	1652:1660	arg1	production					1662:1671	transient production	1652:1671	transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1652:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	0	87	theme	benthamiana	70:80	arg1	Culture					98:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Glycoengineered Nicotiana benthamiana Cell Suspension Culture	44:104	Transient Recombinant Protein Production in Glycoengineered Nicotiana benthamiana Cell Suspension Culture.					
29659495	10	88	theme	plant-specific	1705:1718	arg1	glycans					1720:1726	plant-specific glycans	1705:1726	plant-specific glycans	1705:1726	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	3	89	theme	mammalian	536:544	arg1	proteins					546:553	mammalian proteins	536:553	mammalian proteins	536:553	A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins.					
29659495	8	90	theme	plant	1307:1311	arg1	cells					1313:1317	plant cells	1307:1317	plant cells used	1307:1322	The mass ratio of Agrobacterium to plant cells used was shown to impact recombinant protein expression levels.					
29659495	10	91	theme	proteins	1688:1695	arg1	production					1662:1671	transient production	1652:1671	transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host	1652:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	6	92	theme	plant-specific	899:912	arg1	glycans					914:920	plant-specific glycans	899:920	plant-specific glycans	899:920	To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from &beta;-1,2-xylosyltransferase and &alpha;-1,3-fucosyltransferase knockdown Nicotiana benthamiana.					
29659495	4	93	theme	control	659:665	arg1	structures					674:683	control glycan structures	659:683	control glycan structures	659:683	Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial.					
29659495	10	94	theme	glycoengineered	1733:1747	arg1	host					1768:1771	a glycoengineered plant cell culture host	1731:1771	a glycoengineered plant cell culture host	1731:1771	Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.					
29659495	1	95	theme	therapeutics	278:289	arg1	production					258:267	rapid production	252:267	rapid production of novel therapeutics	252:289	Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed.					
30305463	0	0	theme	Peptide	90:96	arg1	Release					64:70	Extended Release	55:70	Extended Release of Its Conjugated Peptide	55:96	Spontaneously Cleavable Glycosylated Linker Capable of Extended Release of Its Conjugated Peptide.					
30305463	5	1	theme	presented	900:908	arg1	linkers					923:929	glycosylated linkers	910:929	the presented glycosylated linkers	896:929	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	2	from	buffers	995:1001	arg1	preparation					958:968	the preparation	954:968	the preparation of antibodies in aqueous buffers	954:1001	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	0	3	theme	Conjugated	79:88	arg1	Peptide					90:96	Its Conjugated Peptide	75:96	Its Conjugated Peptide	75:96	Spontaneously Cleavable Glycosylated Linker Capable of Extended Release of Its Conjugated Peptide.					
30305463	5	4	theme	glycosylated	910:921	arg1	linkers					923:929	glycosylated linkers	910:929	the presented glycosylated linkers	896:929	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	5	from	antibodies	973:982	arg1	buffers					995:1001	aqueous buffers	987:1001	aqueous buffers	987:1001	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	6	theme	antibodies	973:982	arg1	preparation					958:968	the preparation	954:968	the preparation of antibodies in aqueous buffers	954:1001	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	3	7	theme	first	579:583	arg1	reaction					591:598	a first order reaction	577:598	a first order reaction	577:598	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	3	8	dep	pH	531:532	arg1	the					527:529	the	527:529	the	527:529	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	1	9	theme	peptides	304:311	arg1	solubility					272:281	solubility	272:281	solubility	272:281	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	1	9	theme	peptides	304:311	arg1	stability					287:295	stability	287:295	stability	287:295	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	5	10	theme	aqueous	987:993	arg1	buffers					995:1001	aqueous buffers	987:1001	aqueous buffers	987:1001	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	3	11	theme	linkers	465:471	arg1	Use					454:456	Use	454:456	Use of the linkers	454:471	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	1	12	gly	glycosylated	110:121	arg1	conjugates					123:132	Reversibly glycosylated conjugates	99:132	Reversibly glycosylated conjugates	99:132	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	3	13	theme	buffer	557:562	arg1	temperature					538:548	temperature	538:548	temperature	538:548	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	3	13	theme	buffer	557:562	arg1	pH					531:532	pH	531:532	pH	531:532	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	2	14	theme	ester/thioester-type	411:430	arg1	linker					432:437	ester/thioester-type linker	411:437	ester/thioester-type linker	411:437	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	3	15	theme	order	585:589	arg1	reaction					591:598	a first order reaction	577:598	a first order reaction	577:598	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	1	16	theme	self-cleavable	218:231	arg1	linkers					233:239	self-cleavable linkers	218:239	self-cleavable linkers	218:239	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	2	17	theme	carboxyl	337:344	arg1	peptide					359:365	the peptide	355:365	the peptide	355:365	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	2	17	theme	carboxyl	337:344	arg1	group					346:350	The amino or carboxyl group	324:350	group	346:350	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	5	18	theme	peptide	887:893	arg1	solubility					871:880	the solubility	867:880	the solubility of a peptide	867:893	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	19	theme	reliable	829:836	arg1	glycosylation					810:822	glycosylation	810:822	glycosylation	810:822	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	19	theme	reliable	829:836	arg1	method					838:843	a reliable method	827:843	a reliable method of greatly increasing the solubility of a peptide	827:893	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	1	20	theme	glycosylated	110:121	arg1	conjugates					123:132	Reversibly glycosylated conjugates	99:132	Reversibly glycosylated conjugates	99:132	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	2	21	gly	glycosylated	386:397	arg1	Ascendis					399:406	a glycosylated Ascendis	384:406	a glycosylated Ascendis	384:406	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	1	22	dep	solubility	272:281	arg1	the					268:270	the	268:270	the	268:270	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	2	23	theme	amino	328:332	arg1	peptide					359:365	the peptide	355:365	the peptide	355:365	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	2	23	theme	amino	328:332	arg1	group					346:350	The amino or carboxyl group	324:350	group	346:350	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	1	24	theme	complex-type	159:170	arg1	oligosaccharides					181:196	complex-type N-linked oligosaccharides	159:196	complex-type N-linked oligosaccharides	159:196	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	1	25	from	stability	287:295	arg1	plasma					316:321	plasma	316:321	plasma	316:321	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	5	26	theme	peptides	1049:1056	arg1	case					1015:1018	the case	1011:1018	the case of sparingly soluble antigen peptides	1011:1056	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	3	27	theme	extended	481:488	arg1	release					490:496	extended release	481:496	extended release of the peptides	481:512	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	1	28	link	N-linked	172:179	arg1	oligosaccharides					181:196	complex-type N-linked oligosaccharides	159:196	complex-type N-linked oligosaccharides	159:196	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	3	29	theme	cleavage	611:618	arg1	rate					620:623	their cleavage rate	605:623	their cleavage rate	605:623	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	0	30	theme	Release	64:70	arg1	Capable					44:50	Capable	44:50	Capable	44:50	Spontaneously Cleavable Glycosylated Linker Capable of Extended Release of Its Conjugated Peptide.					
30305463	1	31	from	solubility	272:281	arg1	plasma					316:321	plasma	316:321	plasma	316:321	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	5	32	theme	soluble	1033:1039	arg1	peptides					1049:1056	sparingly soluble antigen peptides	1023:1056	sparingly soluble antigen peptides	1023:1056	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	5	33	gly	glycosylated	910:921	arg1	linkers					923:929	glycosylated linkers	910:929	the presented glycosylated linkers	896:929	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	0	34	theme	Extended	55:62	arg1	Release					64:70	Extended Release	55:70	Extended Release of Its Conjugated Peptide	55:96	Spontaneously Cleavable Glycosylated Linker Capable of Extended Release of Its Conjugated Peptide.					
30305463	3	35	theme	coupling	682:689	arg1	structure					650:658	the structure	646:658	the structure of the peptide-linker coupling	646:689	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	5	36	theme	antigen	1041:1047	arg1	peptides					1049:1056	sparingly soluble antigen peptides	1023:1056	sparingly soluble antigen peptides	1023:1056	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	2	37	theme	peptide	359:365	arg1	peptide					359:365	the peptide	355:365	the peptide	355:365	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	2	37	theme	peptide	359:365	arg1	group					346:350	The amino or carboxyl group	324:350	group	346:350	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	5	38	from	preparation	958:968	arg1	buffers					995:1001	aqueous buffers	987:1001	aqueous buffers	987:1001	Furthermore, because glycosylation is a reliable method of greatly increasing the solubility of a peptide, the presented glycosylated linkers are expected to permit the preparation of antibodies in aqueous buffers even in the case of sparingly soluble antigen peptides.					
30305463	1	39	with	peptides	201:208	arg1	aim					250:252	the aim	246:252	the aim of increasing the solubility and stability of the peptides in plasma	246:321	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
30305463	3	40	theme	peptides	505:512	arg1	release					490:496	extended release	481:496	extended release of the peptides	481:512	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	4	41	theme	peptides	764:771	arg1	use					753:755	the intended use	740:755	the intended use of the peptides to be released	740:786	This tunability will allow optimization towards the intended use of the peptides to be released.					
30305463	4	42	theme	intended	744:751	arg1	use					753:755	the intended use	740:755	the intended use of the peptides to be released	740:786	This tunability will allow optimization towards the intended use of the peptides to be released.					
30305463	3	43	theme	peptide-linker	667:680	arg1	coupling					682:689	the peptide-linker coupling	663:689	the peptide-linker coupling	663:689	Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling.					
30305463	2	44	theme	glycosylated	386:397	arg1	Ascendis					399:406	a glycosylated Ascendis	384:406	a glycosylated Ascendis	384:406	The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively.					
30305463	1	45	theme	N-linked	172:179	arg1	oligosaccharides					181:196	complex-type N-linked oligosaccharides	159:196	complex-type N-linked oligosaccharides	159:196	Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma.					
31776333	3	0	theme	oligosaccharide	389:403	arg1	substrate					405:413	the oligosaccharide substrate	385:413	the oligosaccharide substrate	385:413	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	5	1	theme	defined	800:806	arg1	glycans					808:814	defined glycans	800:814	defined glycans	800:814	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	5	2	theme	polypeptide	703:713	arg1	glycosyltransferase					715:733	a recently discovered site-specific polypeptide glycosyltransferase	667:733	a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm	667:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	5	3	theme	recombinant	865:875	arg1	proteins					877:884	recombinant proteins	865:884	recombinant proteins in the E. coli cytoplasm	865:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	3	4	theme	bacterial	262:270	arg1	platforms					289:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	5	theme	glycoengineering	272:287	arg1	platforms					289:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	6	link	lipid-linked	420:431	arg1	precursor					433:441	a lipid-linked precursor	418:441	a lipid-linked precursor	418:441	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	6	link	lipid-linked	420:431	arg1	secretion					307:315	secretion	307:315	secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate	307:413	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	6	7	theme	self-assembling	1050:1064	arg1	nanomaterials					1066:1078	self-assembling nanomaterials	1050:1078	self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	1050:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	5	8	theme	variable	749:756	arg1	modules					778:784	variable glycosyltransferase modules	749:784	variable glycosyltransferase modules	749:784	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	6	9	theme	glycoengineering	960:975	arg1	platform					977:984	this glycoengineering platform	955:984	this glycoengineering platform	955:984	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	3	10	theme	lipid-linked	420:431	arg1	precursor					433:441	a lipid-linked precursor	418:441	a lipid-linked precursor	418:441	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	10	theme	lipid-linked	420:431	arg1	secretion					307:315	secretion	307:315	secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate	307:413	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	4	11	theme	glycoengineering	568:583	arg1	platform					585:592	a facile glycoengineering platform	559:592	a facile glycoengineering platform that operates in the bacterial cytoplasm	559:633	Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm.					
31776333	3	12	theme	acceptor	324:331	arg1	protein					333:339	the acceptor protein	320:339	the acceptor protein	320:339	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	6	13	theme	cytoplasmic	927:937	arg1	localization					939:950	the cytoplasmic localization	923:950	the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	923:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	1	14	theme	proteins	105:112	arg1	Glycosylation					88:100	Glycosylation	88:100	Glycosylation of proteins	88:112	Glycosylation of proteins profoundly impacts their physical and biological properties.					
31776333	7	15	theme	glycoprotein	1218:1229	arg1	structures					1231:1240	glycoprotein structures	1218:1240	glycoprotein structures	1218:1240	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	4	16	theme	facile	561:566	arg1	platform					585:592	a facile glycoengineering platform	559:592	a facile glycoengineering platform that operates in the bacterial cytoplasm	559:633	Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm.					
31776333	3	17	theme	Established	250:260	arg1	platforms					289:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms	250:297	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	7	18	theme	diverse	1247:1253	arg1	applications					1273:1284	diverse future biomedical applications	1247:1284	diverse future biomedical applications	1247:1284	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	0	19	theme	Cytoplasmic	0:10	arg1	glycoengineering					12:27	Cytoplasmic glycoengineering	0:27	Cytoplasmic glycoengineering	0:27	Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies.					
31776333	5	20	theme	discovered	678:687	arg1	glycosyltransferase					715:733	a recently discovered site-specific polypeptide glycosyltransferase	667:733	a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm	667:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	0	21	theme	glycoprotein	63:74	arg1	assemblies					76:85	nanoscale glycoprotein assemblies	53:85	nanoscale glycoprotein assemblies	53:85	Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies.					
31776333	6	22	theme	multivalent	1011:1021	arg1	nanomaterials					1066:1078	self-assembling nanomaterials	1050:1078	self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	1050:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	6	22	theme	multivalent	1011:1021	arg1	glycostructures					1023:1037	multivalent glycostructures	1011:1037	multivalent glycostructures	1011:1037	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	5	23	theme	origin	843:848	arg1	glycans					808:814	defined glycans	800:814	defined glycans	800:814	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	5	24	theme	site-specific	689:701	arg1	glycosyltransferase					715:733	a recently discovered site-specific polypeptide glycosyltransferase	667:733	a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm	667:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	1	25	gly	Glycosylation	88:100	arg1	proteins					105:112	proteins	105:112	proteins	105:112	Glycosylation of proteins profoundly impacts their physical and biological properties.					
31776333	7	26	theme	future	1255:1260	arg1	applications					1273:1284	diverse future biomedical applications	1247:1284	diverse future biomedical applications	1247:1284	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	3	27	theme	periplasmic	348:358	arg1	space					360:364	the periplasmic space	344:364	the periplasmic space	344:364	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	6	28	theme	glycostructures	1023:1037	arg1	nanomaterials					1066:1078	self-assembling nanomaterials	1050:1078	self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	1050:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	6	28	theme	glycostructures	1023:1037	arg1	variety					1000:1006	a variety	998:1006	a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	998:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	6	28	theme	glycostructures	1023:1037	arg1	glycostructures					1023:1037	multivalent glycostructures	1011:1037	multivalent glycostructures	1011:1037	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	1	29	theme	physical	139:146	arg1	properties					163:172	their physical and biological properties	133:172	their physical and biological properties	133:172	Glycosylation of proteins profoundly impacts their physical and biological properties.					
31776333	0	30	theme	nanoscale	53:61	arg1	assemblies					76:85	nanoscale glycoprotein assemblies	53:85	nanoscale glycoprotein assemblies	53:85	Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies.					
31776333	3	31	theme	protein	333:339	arg1	precursor					433:441	a lipid-linked precursor	418:441	a lipid-linked precursor	418:441	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	31	theme	protein	333:339	arg1	secretion					307:315	secretion	307:315	secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate	307:413	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	2	32	theme	glycoprotein	209:220	arg1	structures					222:231	novel glycoprotein structures	203:231	novel glycoprotein structures	203:231	Yet our ability to engineer novel glycoprotein structures remains limited.					
31776333	5	33	theme	E.	893:894	arg1	cytoplasm					901:909	the E. coli cytoplasm	889:909	the E. coli cytoplasm	889:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	4	34	theme	bacterial	615:623	arg1	cytoplasm					625:633	the bacterial cytoplasm	611:633	the bacterial cytoplasm	611:633	Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm.					
31776333	7	35	theme	biomedical	1262:1271	arg1	applications					1273:1284	diverse future biomedical applications	1247:1284	diverse future biomedical applications	1247:1284	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	2	36	theme	novel	203:207	arg1	structures					222:231	novel glycoprotein structures	203:231	novel glycoprotein structures	203:231	Yet our ability to engineer novel glycoprotein structures remains limited.					
31776333	0	37	theme	assemblies	76:85	arg1	biosynthesis					37:48	biosynthesis	37:48	biosynthesis of nanoscale glycoprotein assemblies	37:85	Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies.					
31776333	6	38	theme	glycan	1114:1119	arg1	epitope					1121:1127	the glycan epitope	1110:1127	the glycan epitope	1110:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	5	39	from	proteins	877:884	arg1	cytoplasm					901:909	the E. coli cytoplasm	889:909	the E. coli cytoplasm	889:909	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	5	40	theme	Glycoli	640:646	arg1	platform					648:655	The Glycoli platform	636:655	The Glycoli platform	636:655	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	3	41	theme	glycan	475:480	arg1	substrates					482:491	glycan substrates	475:491	glycan substrates	475:491	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	5	42	theme	bacterial	820:828	arg1	origin					843:848	bacterial or mammalian origin	820:848	bacterial or mammalian origin	820:848	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	5	43	theme	glycosyltransferase	758:776	arg1	modules					778:784	variable glycosyltransferase modules	749:784	variable glycosyltransferase modules	749:784	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	7	44	theme	powerful	1186:1193	arg1	platform					1195:1202	a powerful platform	1184:1202	a powerful platform for producing glycoprotein structures with diverse future biomedical applications	1184:1284	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	7	44	theme	powerful	1186:1193	arg1	glycoengineering					1164:1179	cytoplasmic glycoengineering	1152:1179	cytoplasmic glycoengineering	1152:1179	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	1	45	theme	biological	152:161	arg1	properties					163:172	their physical and biological properties	133:172	their physical and biological properties	133:172	Glycosylation of proteins profoundly impacts their physical and biological properties.					
31776333	6	46	theme	epitope	1121:1127	arg1	copies					1100:1105	copies	1100:1105	copies of the glycan epitope	1100:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	5	47	theme	mammalian	833:841	arg1	origin					843:848	bacterial or mammalian origin	820:848	bacterial or mammalian origin	820:848	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	7	48	gly	glycoprotein	1218:1229	arg1	glycoprotein					1218:1229	glycoprotein structures	1218:1240	glycoprotein structures	1218:1240	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	3	49	theme	substrate	405:413	arg1	preassembly					370:380	preassembly	370:380	preassembly	370:380	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	3	49	theme	substrate	405:413	arg1	space					360:364	the periplasmic space	344:364	the periplasmic space	344:364	Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively.					
31776333	6	50	theme	platform	977:984	arg1	localization					939:950	the cytoplasmic localization	923:950	the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope	923:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	0	51	gly	glycoprotein	63:74	arg1	glycoprotein					63:74	nanoscale glycoprotein assemblies	53:85	nanoscale glycoprotein assemblies	53:85	Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies.					
31776333	6	52	theme	copies	1100:1105	arg1	hundreds					1088:1095	hundreds	1088:1095	hundreds of copies of the glycan epitope	1088:1127	We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope.					
31776333	5	53	dep	E.	893:894	arg1	coli					896:899	coli	896:899	coli	896:899	The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm.					
31776333	7	54	theme	cytoplasmic	1152:1162	arg1	platform					1195:1202	a powerful platform	1184:1202	a powerful platform for producing glycoprotein structures with diverse future biomedical applications	1184:1284	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	7	54	theme	cytoplasmic	1152:1162	arg1	glycoengineering					1164:1179	cytoplasmic glycoengineering	1152:1179	cytoplasmic glycoengineering	1152:1179	This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.					
31776333	2	55	gly	glycoprotein	209:220	arg1	glycoprotein					209:220	novel glycoprotein structures	203:231	novel glycoprotein structures	203:231	Yet our ability to engineer novel glycoprotein structures remains limited.					
29671439	5	0	theme	Con	636:638	arg1	A					633:633	concanavalin A	620:633	concanavalin A (Con A)	620:641	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	0	theme	Con	636:638	arg1	A					640:640	Con A	636:640	Con A	636:640	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	4	1	theme	mL-1	573:576	arg1	value					549:553	an IC50 value	541:553	an IC50 value of 0.83 ± 0.09 mg mL-1	541:576	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	0	2	theme	inhibitory	84:93	arg1	activity					95:102	ACE inhibitory activity	80:102	ACE inhibitory activity	80:102	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	0	3	link	N-linked	33:40	arg1	glycoprotein					42:53	N-linked glycoprotein	33:53	N-linked glycoprotein	33:53	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	1	4	theme	angiotensin-converting	164:185	arg1	ACE					195:197	ACE	195:197	ACE	195:197	Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized.					
29671439	1	4	theme	angiotensin-converting	164:185	arg1	enzyme					187:192	the angiotensin-converting enzyme	160:192	the angiotensin-converting enzyme (ACE)	160:198	Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized.					
29671439	2	5	gly	glycoproteins	273:285	arg1	glycoproteins					273:285	then the crude glycoproteins	258:285	then the crude glycoproteins	258:285	The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres.					
29671439	4	6	theme	crude	486:490	arg1	active					517:522	active	517:522	active	517:522	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	4	6	theme	crude	486:490	arg1	glycoproteins					499:511	the crude ginger glycoproteins	482:511	the crude ginger glycoproteins	482:511	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	3	7	link	N-linked	355:362	arg1	glycans					364:370	The N-linked glycans	351:370	The N-linked glycans released from the enriched glycoproteins by PNGase F	351:423	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	5	8	theme	N-glycans	714:722	arg1	structures					696:705	the structures	692:705	the structures of the N-glycans released from the ginger glycoproteins	692:761	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	3	9	gly	glycoproteins	399:411	arg1	glycoproteins					399:411	the enriched glycoproteins	386:411	the enriched glycoproteins	386:411	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	2	10	theme	crude	267:271	arg1	glycoproteins					273:285	then the crude glycoproteins	258:285	then the crude glycoproteins	258:285	The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres.					
29671439	3	11	theme	N-linked	355:362	arg1	glycans					364:370	The N-linked glycans	351:370	The N-linked glycans released from the enriched glycoproteins by PNGase F	351:423	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	5	12	theme	concanavalin	620:631	arg1	A					633:633	concanavalin A	620:633	concanavalin A (Con A)	620:641	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	12	theme	concanavalin	620:631	arg1	A					640:640	Con A	636:640	Con A	636:640	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	6	13	theme	ginger	968:973	arg1	glycoproteins					975:987	ginger glycoproteins	968:987	ginger glycoproteins with ACE-inhibitory activity	968:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	3	14	theme	enriched	390:397	arg1	glycoproteins					399:411	the enriched glycoproteins	386:411	the enriched glycoproteins	386:411	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	4	15	theme	ginger	492:497	arg1	active					517:522	active	517:522	active	517:522	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	4	15	theme	ginger	492:497	arg1	glycoproteins					499:511	the crude ginger glycoproteins	482:511	the crude ginger glycoproteins	482:511	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	5	16	attach	released	724:731	arg2	N-glycans					714:722	the N-glycans	710:722	the N-glycans released from the ginger glycoproteins	710:761	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	16	attach	released	724:731	arg1	glycoproteins					749:761	the ginger glycoproteins	738:761	the ginger glycoproteins	738:761	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	17	theme	hybrid-type	828:838	arg1	glycans					840:846	hybrid-type glycans	828:846	hybrid-type glycans	828:846	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	0	18	theme	MALDI-TOF-MS	0:11	arg1	characterization					13:28	MALDI-TOF-MS characterization	0:28	MALDI-TOF-MS characterization of N-linked glycoprotein	0:53	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	4	19	theme	0.83	558:561	arg1	±					563:563	±	563:563	±	563:563	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	2	20	theme	lectin	305:310	arg1	microarrays					312:322	lectin microarrays	305:322	lectin microarrays	305:322	The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres.					
29671439	6	21	theme	glycan	948:953	arg1	structure					955:963	the glycan structure	944:963	the glycan structure of ginger glycoproteins with ACE-inhibitory activity	944:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	0	22	theme	N-linked	33:40	arg1	glycoprotein					42:53	N-linked glycoprotein	33:53	N-linked glycoprotein	33:53	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	5	23	theme	ginger	583:588	arg1	glycoproteins					590:602	The ginger glycoproteins	579:602	The ginger glycoproteins	579:602	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	24	theme	type	784:787	arg1	glycans					789:795	high-mannose type glycans	771:795	high-mannose type glycans	771:795	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	4	25	theme	0.09	565:568	arg1	±					563:563	±	563:563	±	563:563	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	1	26	gly	glycoproteins	135:147	arg1	glycoproteins					135:147	ginger glycoproteins	128:147	ginger glycoproteins	128:147	Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized.					
29671439	6	27	theme	ACE-inhibitory	994:1007	arg1	activity					1009:1016	ACE-inhibitory activity	994:1016	ACE-inhibitory activity	994:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	4	28	gly	glycoproteins	499:511	arg1	active					517:522	active	517:522	active	517:522	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	4	28	gly	glycoproteins	499:511	arg1	glycoproteins					499:511	the crude ginger glycoproteins	482:511	the crude ginger glycoproteins	482:511	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	5	29	theme	fucosylated-type	798:813	arg1	glycans					815:821	fucosylated-type glycans	798:821	fucosylated-type glycans	798:821	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	0	30	theme	glycoprotein	42:53	arg1	characterization					13:28	MALDI-TOF-MS characterization	0:28	MALDI-TOF-MS characterization of N-linked glycoprotein	0:53	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	4	31	dep	mL-1	573:576	arg1	mg					570:571	mg	570:571	mg	570:571	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	5	32	gly	glycoproteins	749:761	arg1	glycoproteins					749:761	the ginger glycoproteins	738:761	the ginger glycoproteins	738:761	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	33	theme	high-mannose	771:782	arg1	glycans					789:795	high-mannose type glycans	771:795	high-mannose type glycans	771:795	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	6	34	theme	study	898:902	arg1	results					882:888	The results	878:888	The results of this study	878:902	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	5	35	theme	ginger	742:747	arg1	glycoproteins					749:761	the ginger glycoproteins	738:761	the ginger glycoproteins	738:761	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	4	36	dep	mg	570:571	arg1	±					563:563	±	563:563	±	563:563	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	6	37	with	glycoproteins	975:987	arg1	activity					1009:1016	ACE-inhibitory activity	994:1016	ACE-inhibitory activity	994:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	3	38	theme	PNGase	416:421	arg1	F					423:423	PNGase F	416:423	PNGase F	416:423	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	5	39	theme	tuberosum	655:663	arg1	lectin					674:679	solanum tuberosum (Potato) lectin	647:679	solanum tuberosum (Potato) lectin (STL)	647:685	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	5	39	theme	tuberosum	655:663	arg1	STL					682:684	STL	682:684	STL	682:684	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	4	40	theme	IC50	544:547	arg1	value					549:553	an IC50 value	541:553	an IC50 value of 0.83 ± 0.09 mg mL-1	541:576	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	0	41	gly	glycoprotein	42:53	arg1	glycoprotein					42:53	N-linked glycoprotein	33:53	N-linked glycoprotein	33:53	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	1	42	theme	ginger	128:133	arg1	glycoproteins					135:147	ginger glycoproteins	128:147	ginger glycoproteins	128:147	Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized.					
29671439	5	43	gly	glycoproteins	590:602	arg1	glycoproteins					590:602	The ginger glycoproteins	579:602	The ginger glycoproteins	579:602	The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS.					
29671439	4	44	with	active	517:522	arg1	value					549:553	an IC50 value	541:553	an IC50 value of 0.83 ± 0.09 mg mL-1	541:576	The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1.					
29671439	0	45	with	ginger	68:73	arg1	activity					95:102	ACE inhibitory activity	80:102	ACE inhibitory activity	80:102	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	1	46	theme	glycoproteins	135:147	arg1	ability					117:123	the ability	113:123	the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE)	113:198	Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized.					
29671439	2	47	theme	magnetic	328:335	arg1	microspheres					337:348	magnetic microspheres	328:348	magnetic microspheres	328:348	The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres.					
29671439	0	48	theme	ACE	80:82	arg1	activity					95:102	ACE inhibitory activity	80:102	ACE inhibitory activity	80:102	MALDI-TOF-MS characterization of N-linked glycoprotein derived from ginger with ACE inhibitory activity.					
29671439	6	49	gly	glycoproteins	975:987	arg1	glycoproteins					975:987	ginger glycoproteins	968:987	ginger glycoproteins with ACE-inhibitory activity	968:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29671439	3	50	attach	released	372:379	arg2	glycans					364:370	The N-linked glycans	351:370	The N-linked glycans released from the enriched glycoproteins by PNGase F	351:423	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	3	50	attach	released	372:379	arg1	glycoproteins					399:411	the enriched glycoproteins	386:411	the enriched glycoproteins	386:411	The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS.					
29671439	6	51	theme	glycoproteins	975:987	arg1	structure					955:963	the glycan structure	944:963	the glycan structure of ginger glycoproteins with ACE-inhibitory activity	944:1016	The results of this study are expected to provide a reference for the glycan structure of ginger glycoproteins with ACE-inhibitory activity.					
29699572	0	0	theme	G.	99:100	arg1	BACKGROUND					102:111	N-glycosylated immunoglobulin G. BACKGROUND	69:111	N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	69:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	3	1	theme	ultra-performance	701:717	arg1	chromatography					726:739	ultra-performance liquid chromatography	701:739	ultra-performance liquid chromatography	701:739	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	0	2	theme	physiologic	214:224	arg1	processes					241:249	multiple physiologic and pathologic processes	205:249	multiple physiologic and pathologic processes	205:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	6	3	dep	correlated	1382:1391	arg1	r = 0.566					1420:1428	r = 0.566	1420:1428	r = 0.566	1420:1428	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	3	4	with	controls	666:673	arg1	IS					604:605	IS	604:605	IS	604:605	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	4	with	controls	666:673	arg1	CAS					648:650	CAS	648:650	CAS	648:650	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	4	with	controls	666:673	arg1	stenosis					638:645	cerebral arterial stenosis	620:645	cerebral arterial stenosis (CAS)	620:651	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	4	with	controls	666:673	arg1	n = 78					608:613	n = 78	608:613	n = 78	608:613	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	7	5	theme	acid	1511:1514	arg1	loss					1482:1485	loss	1482:1485	loss of galactose and sialic acid	1482:1514	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	5	theme	acid	1511:1514	arg1	addition					1528:1535	addition	1528:1535	addition of bisecting GlcNAc	1528:1555	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	0	6	theme	immunoglobulin	84:97	arg1	BACKGROUND					102:111	N-glycosylated immunoglobulin G. BACKGROUND	69:111	N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	69:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	0	7	theme	multiple	205:212	arg1	processes					241:249	multiple physiologic and pathologic processes	205:249	multiple physiologic and pathologic processes	205:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	4	8	from	glycans	765:771	arg1	glycome					803:809	IgG glycome	799:809	IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc	799:873	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	4	9	dep	RESULTS	742:748	arg1	glycans					788:794	10 derived glycans	777:794	10 derived glycans	777:794	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	4	9	dep	RESULTS	742:748	arg1	glycans					765:771	Eleven initial glycans	750:771	Eleven initial glycans	750:771	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	4	10	theme	bisecting	858:866	arg1	GlcNAc					868:873	bisecting GlcNAc	858:873	bisecting GlcNAc	858:873	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	3	11	with	patients	590:597	arg1	IS					604:605	IS	604:605	IS	604:605	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	11	with	patients	590:597	arg1	CAS					648:650	CAS	648:650	CAS	648:650	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	11	with	patients	590:597	arg1	stenosis					638:645	cerebral arterial stenosis	620:645	cerebral arterial stenosis (CAS)	620:651	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	11	with	patients	590:597	arg1	n = 78					608:613	n = 78	608:613	n = 78	608:613	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	0	12	theme	pathologic	230:239	arg1	processes					241:249	multiple physiologic and pathologic processes	205:249	multiple physiologic and pathologic processes	205:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	8	13	theme	chronic	1740:1746	arg1	disease					1748:1754	chronic disease	1740:1754	chronic disease in the future	1740:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	6	14	theme	inflammation	1398:1409	arg1	markers					1411:1417	inflammation markers	1398:1417	inflammation markers	1398:1417	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	0	15	theme	BACKGROUND	102:111	arg1	potential					56:64	the pro-inflammatory potential	35:64	the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	35:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	1	16	dep	stroke	362:367	arg1	IS					370:371	IS	370:371	IS	370:371	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	0	17	gly	N-glycosylated	69:82	arg1	BACKGROUND					102:111	N-glycosylated immunoglobulin G. BACKGROUND	69:111	N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	69:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	7	18	theme	sialic	1504:1509	arg1	acid					1511:1514	sialic acid	1504:1514	sialic acid	1504:1514	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	19	theme	IS	1665:1666	arg1	pathogenesis					1649:1660	the pathogenesis	1645:1660	the pathogenesis of IS	1645:1666	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	8	20	theme	IgG	1669:1671	arg1	biomarkers					1725:1734	clinical useful biomarkers	1709:1734	clinical useful biomarkers for chronic disease in the future	1709:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	8	20	theme	IgG	1669:1671	arg1	profiles					1680:1687	IgG glycan profiles	1669:1687	IgG glycan profiles	1669:1687	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	6	21	theme	correlation	1300:1310	arg1	analysis					1312:1319	The canonical correlation analysis	1286:1319	The canonical correlation analysis	1286:1319	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	7	22	theme	bisecting	1540:1548	arg1	GlcNAc					1550:1555	bisecting GlcNAc	1540:1555	bisecting GlcNAc	1540:1555	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	5	23	dep	area	1130:1133	arg1	CI					1220:1221	95% confidence interval (CI)	1195:1222	95% confidence interval (CI)	1195:1222	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	23	dep	area	1130:1133	arg1	0.732-0.872					1225:1235	0.732-0.872	1225:1235	0.732-0.872	1225:1235	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	23	dep	area	1130:1133	arg1	0.802					1188:1192	0.802	1188:1192	0.802	1188:1192	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	7	24	dep	CONCLUSION	1443:1452	arg1	indicated					1467:1475	indicated	1467:1475	indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS	1467:1666	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	0	25	dep	BACKGROUND	102:111	arg1	participates					189:200	participates	189:200	participates in multiple physiologic and pathologic processes	189:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	0	25	dep	BACKGROUND	102:111	arg1	affects					141:147	affects	141:147	affects protein structure and function	141:178	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	4	26	theme	healthy	930:936	arg1	controls					938:945	healthy controls	930:945	healthy controls	930:945	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	5	27	theme	receiver-operator	1141:1157	arg1	curves					1174:1179	receiver-operator characteristic curves	1141:1179	receiver-operator characteristic curves	1141:1179	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	6	28	theme	canonical	1290:1298	arg1	analysis					1312:1319	The canonical correlation analysis	1286:1319	The canonical correlation analysis	1286:1319	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	0	29	theme	N-glycosylated	69:82	arg1	BACKGROUND					102:111	N-glycosylated immunoglobulin G. BACKGROUND	69:111	N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	69:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	0	30	theme	Ischemic	0:7	arg1	stroke					9:14	Ischemic stroke	0:14	Ischemic stroke	0:14	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	2	31	dep	METHODS	448:454	arg1	aimed					466:470	aimed	466:470	aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population	466:560	METHODS The study aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population.					
29699572	7	32	theme	IgG	1601:1603	arg1	functionality					1605:1617	pro- or anti-inflammatory IgG functionality	1575:1617	pro- or anti-inflammatory IgG functionality	1575:1617	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	33	theme	galactose	1490:1498	arg1	loss					1482:1485	loss	1482:1485	loss of galactose and sialic acid	1482:1514	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	33	theme	galactose	1490:1498	arg1	addition					1528:1535	addition	1528:1535	addition of bisecting GlcNAc	1528:1555	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	34	theme	pro-	1575:1578	arg1	functionality					1605:1617	pro- or anti-inflammatory IgG functionality	1575:1617	pro- or anti-inflammatory IgG functionality	1575:1617	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	8	35	theme	useful	1718:1723	arg1	biomarkers					1725:1734	clinical useful biomarkers	1709:1734	clinical useful biomarkers for chronic disease in the future	1709:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	8	35	theme	useful	1718:1723	arg1	profiles					1680:1687	IgG glycan profiles	1669:1687	IgG glycan profiles	1669:1687	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	3	36	theme	IgG	563:565	arg1	composition					575:585	IgG glycome composition	563:585	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77)	563:682	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	1	37	theme	risk	337:340	arg1	hypertension					434:445	hypertension	434:445	hypertension	434:445	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	37	theme	risk	337:340	arg1	aging					383:387	aging	383:387	aging	383:387	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	37	theme	risk	337:340	arg1	obesity					390:396	obesity	390:396	obesity	390:396	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	37	theme	risk	337:340	arg1	diabetes					420:427	type 2 diabetes	413:427	type 2 diabetes	413:427	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	37	theme	risk	337:340	arg1	factors					342:348	the risk factors	333:348	the risk factors	333:348	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	37	theme	risk	337:340	arg1	dyslipidemia					399:410	dyslipidemia	399:410	dyslipidemia	399:410	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	0	38	theme	protein	149:155	arg1	structure					157:165	protein structure	149:165	protein structure	149:165	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	3	39	theme	glycome	567:573	arg1	composition					575:585	IgG glycome composition	563:585	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77)	563:682	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	4	40	link	derived	780:786	arg1	glycans					788:794	10 derived glycans	777:794	10 derived glycans	777:794	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	3	41	theme	liquid	719:724	arg1	chromatography					726:739	ultra-performance liquid chromatography	701:739	ultra-performance liquid chromatography	701:739	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	2	42	theme	Chinese	543:549	arg1	population					551:560	a Chinese population	541:560	a Chinese population	541:560	METHODS The study aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population.					
29699572	5	43	theme	IgG	1074:1076	arg1	traits					1085:1090	IgG glycan traits	1074:1090	IgG glycan traits	1074:1090	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	4	44	theme	IgG	799:801	arg1	glycome					803:809	IgG glycome	799:809	IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc	799:873	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	7	45	theme	anti-inflammatory	1583:1599	arg1	functionality					1605:1617	pro- or anti-inflammatory IgG functionality	1575:1617	pro- or anti-inflammatory IgG functionality	1575:1617	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	6	46	theme	N-glycan	1344:1351	arg1	structures					1353:1362	initial N-glycan structures	1336:1362	initial N-glycan structures	1336:1362	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	7	47	theme	GlcNAc	1550:1555	arg1	loss					1482:1485	loss	1482:1485	loss of galactose and sialic acid	1482:1514	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	7	47	theme	GlcNAc	1550:1555	arg1	addition					1528:1535	addition	1528:1535	addition of bisecting GlcNAc	1528:1555	CONCLUSION Our findings indicated that loss of galactose and sialic acid, as well as addition of bisecting GlcNAc, might involve in pro- or anti-inflammatory IgG functionality and further contribute to the pathogenesis of IS.					
29699572	3	48	theme	arterial	629:636	arg1	CAS					648:650	CAS	648:650	CAS	648:650	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	48	theme	arterial	629:636	arg1	stenosis					638:645	cerebral arterial stenosis	620:645	cerebral arterial stenosis (CAS)	620:651	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	5	49	theme	interval	1210:1217	arg1	CI					1220:1221	95% confidence interval (CI)	1195:1222	95% confidence interval (CI)	1195:1222	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	50	theme	characteristic	1159:1172	arg1	curves					1174:1179	receiver-operator characteristic curves	1141:1179	receiver-operator characteristic curves	1141:1179	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	6	51	theme	initial	1336:1342	arg1	structures					1353:1362	initial N-glycan structures	1336:1362	initial N-glycan structures	1336:1362	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	1	52	theme	ischemic	353:360	arg1	stroke					362:367	ischemic stroke	353:367	ischemic stroke (IS)	353:372	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	8	53	theme	glycan	1673:1678	arg1	biomarkers					1725:1734	clinical useful biomarkers	1709:1734	clinical useful biomarkers for chronic disease in the future	1709:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	8	53	theme	glycan	1673:1678	arg1	profiles					1680:1687	IgG glycan profiles	1669:1687	IgG glycan profiles	1669:1687	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	1	54	theme	type	413:416	arg1	diabetes					420:427	type 2 diabetes	413:427	type 2 diabetes	413:427	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	0	55	theme	pro-inflammatory	39:54	arg1	potential					56:64	the pro-inflammatory potential	35:64	the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes	35:249	Ischemic stroke is associated with the pro-inflammatory potential of N-glycosylated immunoglobulin G. BACKGROUND Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes.					
29699572	1	56	theme	stroke	362:367	arg1	hypertension					434:445	hypertension	434:445	hypertension	434:445	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	56	theme	stroke	362:367	arg1	aging					383:387	aging	383:387	aging	383:387	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	56	theme	stroke	362:367	arg1	obesity					390:396	obesity	390:396	obesity	390:396	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	56	theme	stroke	362:367	arg1	diabetes					420:427	type 2 diabetes	413:427	type 2 diabetes	413:427	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	56	theme	stroke	362:367	arg1	factors					342:348	the risk factors	333:348	the risk factors	333:348	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	56	theme	stroke	362:367	arg1	dyslipidemia					399:410	dyslipidemia	399:410	dyslipidemia	399:410	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	6	57	dep	r = 0.566	1420:1428	arg1	p < 0.001					1431:1439	p < 0.001	1431:1439	p < 0.001	1431:1439	The canonical correlation analysis indicated that initial N-glycan structures are significantly correlated with inflammation markers (r = 0.566, p < 0.001).					
29699572	5	58	theme	glycan	1078:1083	arg1	traits					1085:1090	IgG glycan traits	1074:1090	IgG glycan traits	1074:1090	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	59	theme	confidence	1199:1208	arg1	CI					1220:1221	95% confidence interval (CI)	1195:1222	95% confidence interval (CI)	1195:1222	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	60	theme	Logistic	1033:1040	arg1	models					1053:1058	Logistic regression models	1033:1058	Logistic regression models incorporating IgG glycan traits	1033:1090	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	8	61	from	disease	1748:1754	arg1	future					1763:1768	future	1763:1768	future	1763:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	5	62	theme	%	1197:1197	arg1	CI					1220:1221	95% confidence interval (CI)	1195:1222	95% confidence interval (CI)	1195:1222	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	4	63	theme	initial	757:763	arg1	glycans					765:771	Eleven initial glycans	750:771	Eleven initial glycans	750:771	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	5	64	theme	regression	1042:1051	arg1	models					1053:1058	Logistic regression models	1033:1058	Logistic regression models incorporating IgG glycan traits	1033:1090	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	3	65	theme	cerebral	620:627	arg1	CAS					648:650	CAS	648:650	CAS	648:650	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	65	theme	cerebral	620:627	arg1	stenosis					638:645	cerebral arterial stenosis	620:645	cerebral arterial stenosis (CAS)	620:651	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	4	66	from	glycans	788:794	arg1	glycome					803:809	IgG glycome	799:809	IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc	799:873	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	3	67	from	composition	575:585	arg1	patients					590:597	patients	590:597	patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75)	590:660	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	67	from	composition	575:585	arg1	n = 77					676:681	n = 77	676:681	n = 77	676:681	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	67	from	composition	575:585	arg1	n = 75					654:659	n = 75	654:659	n = 75	654:659	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	3	67	from	composition	575:585	arg1	controls					666:673	controls	666:673	controls (n = 77)	666:682	IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.					
29699572	1	68	theme	immunoglobulin	278:291	arg1	G					293:293	immunoglobulin G	278:293	immunoglobulin G (IgG) N-glycosylation	278:315	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	1	68	theme	immunoglobulin	278:291	arg1	IgG					296:298	IgG	296:298	IgG	296:298	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	2	69	theme	IgG	511:513	arg1	N-glycosylation					515:529	IgG N-glycosylation	511:529	IgG N-glycosylation	511:529	METHODS The study aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population.					
29699572	8	70	theme	clinical	1709:1716	arg1	biomarkers					1725:1734	clinical useful biomarkers	1709:1734	clinical useful biomarkers for chronic disease in the future	1709:1768	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	8	70	theme	clinical	1709:1716	arg1	profiles					1680:1687	IgG glycan profiles	1669:1687	IgG glycan profiles	1669:1687	IgG glycan profiles may be developed as clinical useful biomarkers for chronic disease in the future.					
29699572	1	71	theme	G	293:293	arg1	N-glycosylation					301:315	immunoglobulin G (IgG) N-glycosylation	278:315	immunoglobulin G (IgG) N-glycosylation	278:315	Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.					
29699572	4	72	theme	derived	780:786	arg1	glycans					788:794	10 derived glycans	777:794	10 derived glycans	777:794	RESULTS Eleven initial glycans and 10 derived glycans in IgG glycome representing galactosylation, sialylation, and bisecting GlcNAc significantly differed between IS patients and CAS and healthy controls after controlling for gender, age, obesity, diabetes, hypertension, and dyslipidemia.					
29699572	5	73	dep	AUC	1252:1254	arg1	%					1266:1266	95% CI	1264:1269	95% CI	1264:1269	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
29699572	5	73	dep	AUC	1252:1254	arg1	0.661-0.819					1272:1282	0.661-0.819	1272:1282	0.661-0.819	1272:1282	Logistic regression models incorporating IgG glycan traits were able to distinguish IS from CAS (area under receiver-operator characteristic curves (AUC), 0.802; 95% confidence interval (CI), 0.732-0.872) and controls (AUC, 0.740; 95% CI, 0.661-0.819).					
31514875	9	0	theme	first	1593:1597	arg1	time					1599:1602	the first time	1589:1602	the first time	1589:1602	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	3	1	gly	glycoproteins	635:647	arg1	glycoproteins					635:647	glycoproteins	635:647	glycoproteins	635:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	6	2	theme	same	1276:1279	arg1	batch					1285:1289	the same hCG batch	1272:1289	the same hCG batch	1272:1289	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	7	3	theme	IMER	1392:1395	arg1	digestions					1397:1406	the IMER digestions	1388:1406	the IMER digestions	1388:1406	Then, the performance of the IMER digestions was compared to in-solution digestions to evaluate the qualitative mapping of the glycosylation.					
31514875	3	4	gly	heterogeneity	618:630	arg1	glycoproteins					635:647	glycoproteins	635:647	glycoproteins	635:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	9	5	gly	glycosylation	1798:1810	arg2	site					1812:1815	the 4 glycosylation site	1792:1815	the 4 glycosylation site	1792:1815	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	9	5	gly	glycosylation	1798:1810	arg2	4					1796:1796	4	1796:1796	4	1796:1796	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	7	6	theme	in-solution	1424:1434	arg1	digestions					1436:1445	in-solution digestions	1424:1445	in-solution digestions	1424:1445	Then, the performance of the IMER digestions was compared to in-solution digestions to evaluate the qualitative mapping of the glycosylation.					
31514875	1	7	theme	proteins	175:182	arg1	PTMs					166:169	PTMs	166:169	PTMs	166:169	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	1	7	theme	proteins	175:182	arg1	modifications					151:163	post-translational modifications	132:163	post-translational modifications (PTMs) of proteins	132:182	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	9	8	theme	trypsin	1628:1634	arg1	complementarity					1609:1623	the complementarity	1605:1623	the complementarity of trypsin and pepsin	1605:1645	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	9	9	theme	pepsin	1640:1645	arg1	complementarity					1609:1623	the complementarity	1605:1623	the complementarity of trypsin and pepsin	1605:1645	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	10	10	theme	protein	1881:1887	arg1	glycosylation					1862:1874	the glycosylation	1858:1874	the glycosylation of a protein	1858:1887	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	4	11	theme	tandem	911:916	arg1	nanoLC-MS/MS					937:948	nanoLC-MS/MS	937:948	nanoLC-MS/MS	937:948	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	11	theme	tandem	911:916	arg1	spectrometry					923:934	tandem mass spectrometry	911:934	tandem mass spectrometry (nanoLC-MS/MS)	911:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	6	12	theme	digestion	1221:1229	arg1	repeatability					1200:1212	The repeatability	1196:1212	The repeatability of the digestion with trypsin-based IMER	1196:1253	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	5	13	from	separation	1184:1193	arg1	time					1137:1140	the retention time	1123:1140	the retention time of glycopeptides in reversed phase nanoLC separation	1123:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	1	14	theme	proteomics	214:223	arg1	strategy					225:232	bottom-up proteomics strategy	204:232	bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule	204:302	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	4	15	theme	digests	863:869	arg1	analysis					847:854	the analysis	843:854	the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS)	843:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	5	16	gly	glycopeptides	1145:1157	arg2	glycopeptides					1145:1157	glycopeptides	1145:1157	glycopeptides in reversed phase nanoLC separation	1145:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	4	17	theme	nanoliquid	874:883	arg1	chromatography					885:898	nanoliquid chromatography	874:898	nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS)	874:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	6	18	theme	hCG	1281:1283	arg1	batch					1285:1289	the same hCG batch	1272:1289	the same hCG batch	1272:1289	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	1	19	theme	post-translational	132:149	arg1	PTMs					166:169	PTMs	166:169	PTMs	166:169	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	1	19	theme	post-translational	132:149	arg1	modifications					151:163	post-translational modifications	132:163	post-translational modifications (PTMs) of proteins	132:182	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	10	20	theme	hCG-based	1932:1940	arg1	drugs					1942:1946	two hCG-based drugs	1928:1946	two hCG-based drugs	1928:1946	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	10	20	theme	hCG-based	1932:1940	arg1	Pregnyl®					1964:1971	Pregnyl®	1964:1971	Pregnyl®	1964:1971	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	10	20	theme	hCG-based	1932:1940	arg1	Ovitrelle®					1949:1958	Ovitrelle®	1949:1958	Ovitrelle®	1949:1958	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	3	21	theme	mapping	587:593	arg1	reliability					559:569	the reliability	555:569	the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins	555:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	1	22	theme	biomolecule	292:302	arg1	digestion					275:283	the enzymatic digestion	261:283	the enzymatic digestion of the biomolecule	261:302	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	0	23	theme	glycosylation	75:87	arg1	heterogeneity					89:101	the glycosylation heterogeneity	71:101	the glycosylation heterogeneity of a protein	71:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	9	24	gly	glycosylation	1741:1753	arg2	4					1739:1739	4	1739:1739	4	1739:1739	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	9	24	gly	glycosylation	1741:1753	arg2	site					1755:1758	2 out of 4 glycosylation site	1730:1758	2 out of 4 glycosylation site	1730:1758	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	5	25	theme	identified	1063:1072	arg1	glycans					1074:1080	the identified glycans	1059:1080	the identified glycans	1059:1080	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	6	26	dep	glycans	1344:1350	arg1	%					1360:1360	%	1360:1360	%	1360:1360	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	3	27	theme	Immobilized	431:441	arg1	IMERs					460:464	IMERs	460:464	IMERs	460:464	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	3	27	theme	Immobilized	431:441	arg1	Reactors					450:457	two Immobilized Enzyme Reactors	427:457	two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease	427:502	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	0	28	theme	protein	108:114	arg1	heterogeneity					89:101	the glycosylation heterogeneity	71:101	the glycosylation heterogeneity of a protein	71:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	0	29	gly	heterogeneity	89:101	arg1	protein					108:114	a protein	106:114	a protein	106:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	4	30	theme	Chorionic	724:732	arg1	Gonadotropin					734:745	human Chorionic Gonadotropin	718:745	human Chorionic Gonadotropin hormone (hCG)	718:759	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	2	31	theme	large	381:385	arg1	heterogeneity					398:410	its large structural heterogeneity	377:410	its large structural heterogeneity	377:410	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	5	32	theme	glycopeptides	1145:1157	arg1	time					1137:1140	the retention time	1123:1140	the retention time of glycopeptides in reversed phase nanoLC separation	1123:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	6	33	dep	%	1360:1360	arg1	75					1358:1359	75	1358:1359	75	1358:1359	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	4	34	theme	hormone	747:753	arg1	digestion					705:713	the digestion	701:713	the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS)	701:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	0	35	theme	Immobilized	15:25	arg1	Reactors					34:41	Immobilized Enzyme Reactors	15:41	Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein	15:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	5	36	theme	reversed	1162:1169	arg1	separation					1184:1193	reversed phase nanoLC separation	1162:1193	reversed phase nanoLC separation	1162:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	3	37	theme	glycoproteins	635:647	arg1	heterogeneity					618:630	the N-glycosylation heterogeneity	598:630	the N-glycosylation heterogeneity of glycoproteins	598:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	0	38	theme	Reactors	34:41	arg1	Development					0:10	Development	0:10	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.	0:115	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	4	39	gly	glycoprotein	764:775	arg1	hormone					747:753	human Chorionic Gonadotropin hormone	718:753	human Chorionic Gonadotropin hormone (hCG)	718:759	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	39	gly	glycoprotein	764:775	arg1	glycoprotein					764:775	a glycoprotein	762:775	a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS)	762:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	7	40	theme	glycosylation	1490:1502	arg1	mapping					1475:1481	the qualitative mapping	1459:1481	the qualitative mapping of the glycosylation	1459:1502	Then, the performance of the IMER digestions was compared to in-solution digestions to evaluate the qualitative mapping of the glycosylation.					
31514875	3	41	theme	heterogeneity	618:630	arg1	mapping					587:593	the specific mapping	574:593	the specific mapping of the N-glycosylation heterogeneity of glycoproteins	574:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	2	42	theme	PTM	350:352	arg1	PTM					350:352	the most challenging PTM	329:352	the most challenging PTM to characterize due to its large structural heterogeneity	329:410	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	2	42	theme	PTM	350:352	arg1	one					322:324	one	322:324	one	322:324	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	6	43	theme	trypsin-based	1236:1248	arg1	IMER					1250:1253	trypsin-based IMER	1236:1253	trypsin-based IMER	1236:1253	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	2	44	theme	most	333:336	arg1	PTM					350:352	the most challenging PTM	329:352	the most challenging PTM to characterize due to its large structural heterogeneity	329:410	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	7	45	theme	qualitative	1463:1473	arg1	mapping					1475:1481	the qualitative mapping	1459:1481	the qualitative mapping of the glycosylation	1459:1502	Then, the performance of the IMER digestions was compared to in-solution digestions to evaluate the qualitative mapping of the glycosylation.					
31514875	9	46	theme	glycosylation	1798:1810	arg1	site					1812:1815	the 4 glycosylation site	1792:1815	the 4 glycosylation site	1792:1815	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	3	47	used	used	509:512	arg2	IMERs					460:464	IMERs	460:464	IMERs	460:464	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	3	47	used	used	509:512	arg2	Reactors					450:457	two Immobilized Enzyme Reactors	427:457	two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease	427:502	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	5	48	from	time	1137:1140	arg1	separation					1184:1193	reversed phase nanoLC separation	1162:1193	reversed phase nanoLC separation	1162:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	5	49	theme	nanoLC	1177:1182	arg1	separation					1184:1193	reversed phase nanoLC separation	1162:1193	reversed phase nanoLC separation	1162:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	4	50	theme	prior	834:838	arg1	N-					799:800	four N- and four O-glycosylation sites	794:831	N-	799:800	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	10	51	theme	IMERs	1835:1839	arg1	potential					1822:1830	The potential	1818:1830	The potential of IMERs for the study of the glycosylation of a protein	1818:1887	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	3	52	theme	first	522:526	arg1	time					528:531	the first time	518:531	the first time	518:531	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	1	53	theme	enzymatic	265:273	arg1	digestion					275:283	the enzymatic digestion	261:283	the enzymatic digestion of the biomolecule	261:302	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	7	54	theme	digestions	1397:1406	arg1	performance					1373:1383	the performance	1369:1383	the performance of the IMER digestions	1369:1406	Then, the performance of the IMER digestions was compared to in-solution digestions to evaluate the qualitative mapping of the glycosylation.					
31514875	5	55	dep	method	1019:1024	arg1	control					1029:1035	control	1029:1035	to control the identification of the identified glycans	1026:1080	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	10	56	theme	glycosylation	1862:1874	arg1	study					1849:1853	the study	1845:1853	the study of the glycosylation of a protein	1845:1887	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	4	57	theme	O-glycosylation	811:825	arg1	sites					827:831	four N- and four O-glycosylation sites	794:831	sites	827:831	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	5	58	from	glycopeptides	1145:1157	arg1	separation					1184:1193	reversed phase nanoLC separation	1162:1193	reversed phase nanoLC separation	1162:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	6	59	with	batches	1316:1322	arg1	glycans					1344:1350	common located glycans	1329:1350	common located glycans up to 75%	1329:1360	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	4	60	gly	O-glycosylation	811:825	arg2	four					806:809	four	806:809	four	806:809	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	60	gly	O-glycosylation	811:825	arg2	sites					827:831	four N- and four O-glycosylation sites	794:831	sites	827:831	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	6	61	theme	independent	1304:1314	arg1	batches					1316:1322	three independent batches	1298:1322	three independent batches with common located glycans up to 75%	1298:1360	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	1	62	theme	bottom-up	204:212	arg1	strategy					225:232	bottom-up proteomics strategy	204:232	bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule	204:302	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	10	63	gly	glycosylation	1862:1874	arg1	protein					1881:1887	a protein	1879:1887	a protein	1879:1887	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	9	64	theme	glycosylation	1671:1683	arg1	mapping					1685:1691	the glycosylation mapping	1667:1691	the glycosylation mapping	1667:1691	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	10	65	theme	drugs	1942:1946	arg1	comparison					1914:1923	the comparison	1910:1923	the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®	1910:1971	The potential of IMERs for the study of the glycosylation of a protein was illustrated with the comparison of two hCG-based drugs, Ovitrelle® and Pregnyl®.					
31514875	3	66	theme	specific	578:585	arg1	mapping					587:593	the specific mapping	574:593	the specific mapping of the N-glycosylation heterogeneity of glycoproteins	574:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	1	67	theme	modifications	151:163	arg1	mapping					121:127	The mapping	117:127	The mapping of post-translational modifications (PTMs) of proteins	117:182	The mapping of post-translational modifications (PTMs) of proteins can be addressed by bottom-up proteomics strategy using proteases to achieve the enzymatic digestion of the biomolecule.					
31514875	6	68	with	digestion	1221:1229	arg1	IMER					1250:1253	trypsin-based IMER	1236:1253	trypsin-based IMER	1236:1253	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	4	69	theme	the	669:671	arg1	performance					654:664	The performance	650:664	The performance of the supports	650:680	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	0	70	theme	heterogeneity	89:101	arg1	characterization					51:66	the characterization	47:66	the characterization of the glycosylation heterogeneity of a protein	47:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	4	71	theme	mass	918:921	arg1	nanoLC-MS/MS					937:948	nanoLC-MS/MS	937:948	nanoLC-MS/MS	937:948	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	71	theme	mass	918:921	arg1	spectrometry					923:934	tandem mass spectrometry	911:934	tandem mass spectrometry (nanoLC-MS/MS)	911:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	6	72	theme	located	1336:1342	arg1	glycans					1344:1350	common located glycans	1329:1350	common located glycans up to 75%	1329:1360	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	5	73	theme	glycans	1074:1080	arg1	identification					1041:1054	the identification	1037:1054	the identification of the identified glycans	1037:1080	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	6	74	dep	75	1358:1359	arg1	to					1355:1356	to	1355:1356	to	1355:1356	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	0	75	gly	glycosylation	75:87	arg1	protein					108:114	a protein	106:114	a protein	106:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	3	76	theme	Enzyme	443:448	arg1	IMERs					460:464	IMERs	460:464	IMERs	460:464	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	3	76	theme	Enzyme	443:448	arg1	Reactors					450:457	two Immobilized Enzyme Reactors	427:457	two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease	427:502	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	9	77	theme	glycosylation	1741:1753	arg1	site					1755:1758	2 out of 4 glycosylation site	1730:1758	2 out of 4 glycosylation site	1730:1758	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	4	78	theme	human	718:722	arg1	Gonadotropin					734:745	human Chorionic Gonadotropin	718:745	human Chorionic Gonadotropin hormone (hCG)	718:759	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	0	79	theme	Enzyme	27:32	arg1	Reactors					34:41	Immobilized Enzyme Reactors	15:41	Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein	15:114	Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein.					
31514875	3	80	dep	trypsin	476:482	arg1	protease					495:502	protease	495:502	protease	495:502	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	4	81	theme	Gonadotropin	734:745	arg1	hCG					756:758	hCG	756:758	hCG	756:758	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	81	theme	Gonadotropin	734:745	arg1	hormone					747:753	human Chorionic Gonadotropin hormone	718:753	human Chorionic Gonadotropin hormone (hCG)	718:759	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	4	81	theme	Gonadotropin	734:745	arg1	glycoprotein					764:775	a glycoprotein	762:775	a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS)	762:949	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	5	82	theme	phase	1171:1175	arg1	separation					1184:1193	reversed phase nanoLC separation	1162:1193	reversed phase nanoLC separation	1162:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	5	83	theme	nanoLC-MS/MS	986:997	arg1	repeatability					965:977	the repeatability	961:977	the repeatability of the nanoLC-MS/MS	961:997	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	3	84	theme	N-glycosylation	602:616	arg1	heterogeneity					618:630	the N-glycosylation heterogeneity	598:630	the N-glycosylation heterogeneity of glycoproteins	598:647	In this work, two Immobilized Enzyme Reactors (IMERs) based on trypsin and pepsin protease were used for the first time to fasten and improve the reliability of the specific mapping of the N-glycosylation heterogeneity of glycoproteins.					
31514875	6	85	theme	common	1329:1334	arg1	glycans					1344:1350	common located glycans	1329:1350	common located glycans up to 75%	1329:1360	The repeatability of the digestion with trypsin-based IMER was evaluated on the same hCG batch and on three independent batches with common located glycans up to 75%.					
31514875	5	86	theme	retention	1127:1135	arg1	time					1137:1140	the retention time	1123:1140	the retention time of glycopeptides in reversed phase nanoLC separation	1123:1193	Firstly, the repeatability of the nanoLC-MS/MS was evaluated and a method to control the identification of the identified glycans was developed to validate them regarding the retention time of glycopeptides in reversed phase nanoLC separation.					
31514875	8	87	theme	common	1539:1544	arg1	glycans					1546:1552	42 out of 45 common glycans	1526:1552	42 out of 45 common glycans between both digestions modes	1526:1582	It has given rise to 42 out of 45 common glycans between both digestions modes.					
31514875	2	88	theme	challenging	338:348	arg1	PTM					350:352	the most challenging PTM	329:352	the most challenging PTM to characterize due to its large structural heterogeneity	329:410	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	9	89	from	identifications	1711:1725	arg1	site					1755:1758	2 out of 4 glycosylation site	1730:1758	2 out of 4 glycosylation site	1730:1758	For the first time, the complementarity of trypsin and pepsin was illustrated for the glycosylation mapping as trypsin led to identifications on 2 out of 4 glycosylation site while pepsin was informative on the 4 glycosylation site.					
31514875	4	90	dep	the	669:671	arg1	supports					673:680	supports	673:680	supports	673:680	The performance of the supports was evaluated with the digestion of human Chorionic Gonadotropin hormone (hCG), a glycoprotein characterized by four N- and four O-glycosylation sites, prior to the analysis of the digests by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).					
31514875	2	91	theme	structural	387:396	arg1	heterogeneity					398:410	its large structural heterogeneity	377:410	its large structural heterogeneity	377:410	Glycosylation is one of the most challenging PTM to characterize due to its large structural heterogeneity.					
31514875	8	92	theme	digestions	1567:1576	arg1	modes					1578:1582	both digestions modes	1562:1582	both digestions modes	1562:1582	It has given rise to 42 out of 45 common glycans between both digestions modes.					
31180129	7	0	theme	several	1227:1233	arg1	CAZome					1094:1099	the CAZome	1090:1099	the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1	1090:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	0	theme	several	1227:1233	arg1	enzymes					1254:1260	several putative and known enzymes	1227:1260	several putative and known enzymes with activities consistent with the modification of mucin	1227:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	2	1	theme	mucus	353:357	arg1	barrier					359:365	the protective mucus barrier	338:365	the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins	338:466	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	3	2	theme	formidable	538:547	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	2	theme	formidable	538:547	arg1	barrier					558:564	a formidable gel-like barrier	536:564	a formidable gel-like barrier against invading pathogens	536:591	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	2	theme	formidable	538:547	arg1	glycans					509:515	O-linked glycans	500:515	O-linked glycans	500:515	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	12	3	theme	chicken	2095:2101	arg1	O-glycans					2109:2117	chicken mucin O-glycans	2095:2117	chicken mucin O-glycans	2095:2117	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	2	4	theme	perfringens	275:285	arg1	ability					261:267	The ability	257:267	The ability of C. perfringens to incite NE	257:298	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	1	5	theme	etiological	200:210	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	5	theme	etiological	200:210	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	5	theme	etiological	200:210	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	4	6	theme	O-linked	670:677	arg1	glycans					679:685	avian O-linked glycans	664:685	avian O-linked glycans	664:685	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	7	7	with	consistent	1278:1287	arg1	modification					1298:1309	the modification	1294:1309	the modification of mucin	1294:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	3	8	theme	invading	574:581	arg1	pathogens					583:591	invading pathogens	574:591	invading pathogens	574:591	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	13	9	theme	C.	2322:2323	arg1	CP1					2337:2339	C. perfringens CP1	2322:2339	C. perfringens CP1	2322:2339	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	3	10	link	O-linked	500:507	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	10	link	O-linked	500:507	arg1	glycans					509:515	O-linked glycans	500:515	O-linked glycans	500:515	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	10	link	O-linked	500:507	arg1	barrier					558:564	a formidable gel-like barrier	536:564	a formidable gel-like barrier against invading pathogens	536:591	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	7	11	dep	collection	1118:1127	arg1	i.e.					1102:1105	i.e.	1102:1105	i.e.	1102:1105	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	10	12	theme	carbon	1776:1781	arg1	sources					1783:1789	sole carbon sources	1771:1789	sole carbon sources	1771:1789	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	10	12	theme	carbon	1776:1781	arg1	monosaccharides					1752:1766	these monosaccharides	1746:1766	these monosaccharides	1746:1766	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	1	13	theme	enteritis	230:238	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	13	theme	enteritis	230:238	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	13	theme	enteritis	230:238	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	4	14	link	O-linked	670:677	arg1	glycans					679:685	avian O-linked glycans	664:685	avian O-linked glycans	664:685	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	8	15	dep	gallus	1385:1390	arg1	mucus					1403:1407	Gallus gallus domesticus mucus	1378:1407	Gallus gallus domesticus mucus	1378:1407	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	10	16	theme	C.	1807:1808	arg1	CP1					1822:1824	C. perfringens CP1	1807:1824	C. perfringens CP1	1807:1824	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	13	17	theme	predicted	2289:2297	arg1	potential					2309:2317	the predicted metabolic potential	2285:2317	the predicted metabolic potential of C. perfringens CP1	2285:2339	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	8	18	theme	chromatography-mass	1477:1495	arg1	spectrometry					1497:1508	gas chromatography-mass spectrometry	1473:1508	gas chromatography-mass spectrometry	1473:1508	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	4	19	theme	other	724:728	arg1	vertebrates					730:740	other vertebrates	724:740	other vertebrates	724:740	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	10	20	theme	sole	1771:1774	arg1	sources					1783:1789	sole carbon sources	1771:1789	sole carbon sources	1771:1789	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	10	20	theme	sole	1771:1774	arg1	monosaccharides					1752:1766	these monosaccharides	1746:1766	these monosaccharides	1746:1766	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	1	21	from	agent	212:216	arg1	poultry					248:254	poultry	248:254	poultry	248:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	9	22	theme	Chicken	1555:1561	arg1	monosaccharides					1569:1583	Chicken mucin monosaccharides	1555:1583	Chicken mucin monosaccharides	1555:1583	Chicken mucin monosaccharides included l-fucose (Fuc), d-mannose (Man), d-galactose (Gal), N-acetyl-d-galactosamine (GalNAc), N-acetyl-d-glucosamine (GlcNAc), and Neu5Ac (sialic acid).					
31180129	6	23	theme	mucus	1041:1045	arg1	layer					1047:1051	the poultry intestinal mucus layer	1018:1051	the poultry intestinal mucus layer	1018:1051	The mechanism by which C. perfringens penetrates the poultry intestinal mucus layer during NE is still unknown.					
31180129	0	24	theme	O-glycan	62:69	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	3	25	theme	carbon	607:612	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	25	theme	carbon	607:612	arg1	source					614:619	a rich carbon source	600:619	a rich carbon source for mucolytic bacteria	600:642	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	5	26	theme	mucins	900:905	arg1	charge					890:895	the overall negative charge	869:895	the overall negative charge of mucins	869:905	These modifications increase the overall negative charge of mucins and are believed to impede colonization by enteric pathogens.					
31180129	6	27	theme	poultry	1022:1028	arg1	layer					1047:1051	the poultry intestinal mucus layer	1018:1051	the poultry intestinal mucus layer	1018:1051	The mechanism by which C. perfringens penetrates the poultry intestinal mucus layer during NE is still unknown.					
31180129	8	28	theme	chromatography-mass	1521:1539	arg1	spectrometry					1541:1552	liquid chromatography-mass spectrometry	1514:1552	liquid chromatography-mass spectrometry	1514:1552	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	7	29	theme	strain	1208:1213	arg1	CP1					1215:1217	C. perfringens strain CP1	1193:1217	C. perfringens strain CP1	1193:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	4	30	theme	sulfated	761:768	arg1	monosaccharides					770:784	sulfated monosaccharides	761:784	sulfated monosaccharides	761:784	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	7	31	theme	C.	1193:1194	arg1	CP1					1215:1217	C. perfringens strain CP1	1193:1217	C. perfringens strain CP1	1193:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	2	32	theme	incite	290:295	arg1	NE					297:298	incite NE	290:298	incite NE	290:298	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	6	33	theme	C.	992:993	arg1	perfringens					995:1005	C. perfringens	992:1005	C. perfringens	992:1005	The mechanism by which C. perfringens penetrates the poultry intestinal mucus layer during NE is still unknown.					
31180129	12	34	theme	Neu5Ac	2226:2231	arg1	removal					2215:2221	preferential removal	2202:2221	preferential removal of Neu5Ac	2202:2231	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	1	35	theme	opportunistic	155:167	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	35	theme	opportunistic	155:167	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	35	theme	opportunistic	155:167	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	0	36	theme	modification	71:82	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.	0:110	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	0	37	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.	0:110	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	5	38	theme	enteric	950:956	arg1	pathogens					958:966	enteric pathogens	950:966	enteric pathogens	950:966	These modifications increase the overall negative charge of mucins and are believed to impede colonization by enteric pathogens.					
31180129	2	39	theme	glycosylated	432:443	arg1	proteins					445:452	heavily glycosylated proteins	424:452	heavily glycosylated proteins called mucins	424:466	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	11	40	dep	demonstrated	1898:1909	arg1	grew					1926:1929	grew	1926:1929	demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins	1898:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	4	41	theme	sialic	826:831	arg1	Neu5Ac					818:823	Neu5Ac	818:823	Neu5Ac	818:823	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	41	theme	sialic	826:831	arg1	acid					833:836	sialic acid	826:836	sialic acid	826:836	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	0	42	theme	chicken	31:37	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	13	43	dep	C.	2322:2323	arg1	perfringens					2325:2335	perfringens	2325:2335	perfringens	2325:2335	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	2	44	theme	protective	342:351	arg1	barrier					359:365	the protective mucus barrier	338:365	the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins	338:466	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	7	45	theme	proteins	1132:1139	arg1	collection					1118:1127	the total collection	1108:1127	the total collection of proteins encoded within a genome active on carbohydrates	1108:1187	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	46	theme	consistent	1278:1287	arg1	activities					1267:1276	activities	1267:1276	activities consistent with the modification of mucin	1267:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	11	47	theme	different	1934:1942	arg1	preparations					1957:1968	different native-state preparations	1934:1968	different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins	1934:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	12	48	theme	C.	2124:2125	arg1	perfringens					2127:2137	C. perfringens	2124:2137	C. perfringens	2124:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	12	49	theme	subsequent	2143:2152	arg1	analysis					2154:2161	subsequent analysis	2143:2161	subsequent analysis of the glycans	2143:2176	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	11	50	theme	intestinal	1973:1982	arg1	mucins					1984:1989	intestinal mucins	1973:1989	intestinal mucins	1973:1989	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	11	50	theme	intestinal	1973:1982	arg1	mucus					2035:2039	chicken mucus	2027:2039	chicken mucus	2027:2039	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	11	50	theme	intestinal	1973:1982	arg1	mucins					2054:2059	chicken mucins	2046:2059	chicken mucins	2046:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	11	50	theme	intestinal	1973:1982	arg1	mucins					2019:2024	porcine mucins	2011:2024	porcine mucins	2011:2024	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	13	51	theme	CP1	2337:2339	arg1	potential					2309:2317	the predicted metabolic potential	2285:2317	the predicted metabolic potential of C. perfringens CP1	2285:2339	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	5	52	theme	overall	873:879	arg1	charge					890:895	the overall negative charge	869:895	the overall negative charge of mucins	869:905	These modifications increase the overall negative charge of mucins and are believed to impede colonization by enteric pathogens.					
31180129	2	53	gly	glycosylated	432:443	arg1	proteins					445:452	heavily glycosylated proteins	424:452	heavily glycosylated proteins called mucins	424:466	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	7	54	theme	known	1248:1252	arg1	CAZome					1094:1099	the CAZome	1090:1099	the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1	1090:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	54	theme	known	1248:1252	arg1	enzymes					1254:1260	several putative and known enzymes	1227:1260	several putative and known enzymes with activities consistent with the modification of mucin	1227:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	55	theme	total	1112:1116	arg1	collection					1118:1127	the total collection	1108:1127	the total collection of proteins encoded within a genome active on carbohydrates	1108:1187	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	3	56	theme	O-linked	500:507	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	56	theme	O-linked	500:507	arg1	glycans					509:515	O-linked glycans	500:515	O-linked glycans	500:515	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	56	theme	O-linked	500:507	arg1	barrier					558:564	a formidable gel-like barrier	536:564	a formidable gel-like barrier against invading pathogens	536:591	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	7	57	theme	putative	1235:1242	arg1	CAZome					1094:1099	the CAZome	1090:1099	the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1	1090:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	57	theme	putative	1235:1242	arg1	enzymes					1254:1260	several putative and known enzymes	1227:1260	several putative and known enzymes with activities consistent with the modification of mucin	1227:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	11	58	theme	mucins	1984:1989	arg1	preparations					1957:1968	different native-state preparations	1934:1968	different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins	1934:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	7	59	with	enzymes	1254:1260	arg1	activities					1267:1276	activities	1267:1276	activities consistent with the modification of mucin	1267:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	5	60	theme	negative	881:888	arg1	charge					890:895	the overall negative charge	869:895	the overall negative charge of mucins	869:905	These modifications increase the overall negative charge of mucins and are believed to impede colonization by enteric pathogens.					
31180129	1	61	theme	principal	190:198	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	61	theme	principal	190:198	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	61	theme	principal	190:198	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	0	62	theme	intestinal	45:54	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	12	63	theme	O-glycans	2109:2117	arg1	analysis					2154:2161	subsequent analysis	2143:2161	subsequent analysis of the glycans	2143:2176	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	12	63	theme	O-glycans	2109:2117	arg1	incubation					2081:2090	anaerobic incubation	2071:2090	anaerobic incubation of chicken mucin O-glycans with C. perfringens	2071:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	13	64	theme	metabolic	2299:2307	arg1	potential					2309:2317	the predicted metabolic potential	2285:2317	the predicted metabolic potential of C. perfringens CP1	2285:2339	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	11	65	theme	porcine	2011:2017	arg1	mucins					2019:2024	porcine mucins	2011:2024	porcine mucins	2011:2024	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	4	66	dep	acid	812:815	arg1	Neu5Ac					818:823	Neu5Ac	818:823	Neu5Ac	818:823	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	66	dep	acid	812:815	arg1	acid					833:836	sialic acid	826:836	sialic acid	826:836	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	67	theme	avian	664:668	arg1	glycans					679:685	avian O-linked glycans	664:685	avian O-linked glycans	664:685	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	3	68	theme	gel-like	549:556	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	68	theme	gel-like	549:556	arg1	barrier					558:564	a formidable gel-like barrier	536:564	a formidable gel-like barrier against invading pathogens	536:591	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	68	theme	gel-like	549:556	arg1	glycans					509:515	O-linked glycans	500:515	O-linked glycans	500:515	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	1	69	theme	necrotic	221:228	arg1	enteritis					230:238	necrotic enteritis	221:238	necrotic enteritis (NE)	221:243	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	69	theme	necrotic	221:228	arg1	NE					241:242	NE	241:242	NE	241:242	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	12	70	theme	mucin	2103:2107	arg1	O-glycans					2109:2117	chicken mucin O-glycans	2095:2117	chicken mucin O-glycans	2095:2117	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	11	71	theme	chicken	2027:2033	arg1	mucus					2035:2039	chicken mucus	2027:2039	chicken mucus	2027:2039	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	8	72	theme	gas	1473:1475	arg1	spectrometry					1497:1508	gas chromatography-mass spectrometry	1473:1508	gas chromatography-mass spectrometry	1473:1508	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	10	73	dep	C.	1807:1808	arg1	perfringens					1810:1820	perfringens	1810:1820	perfringens	1810:1820	Using these monosaccharides as sole carbon sources, we showed that C. perfringens CP1 grew on Neu5Ac, Man, Gal, and GlcNAc but not on Fuc and GalNAc.					
31180129	11	74	theme	native-state	1944:1955	arg1	preparations					1957:1968	different native-state preparations	1934:1968	different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins	1934:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	2	75	theme	C.	272:273	arg1	perfringens					275:285	C. perfringens	272:285	C. perfringens	272:285	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	1	76	theme	Gram-positive	141:153	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	76	theme	Gram-positive	141:153	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	76	theme	Gram-positive	141:153	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	11	77	theme	mucus	1995:1999	arg1	preparations					1957:1968	different native-state preparations	1934:1968	different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins	1934:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	11	78	theme	chicken	2046:2052	arg1	mucins					2054:2059	chicken mucins	2046:2059	chicken mucins	2046:2059	We also demonstrated C. perfringens grew on different native-state preparations of intestinal mucins and mucus including porcine mucins, chicken mucus, and chicken mucins.					
31180129	8	79	theme	liquid	1514:1519	arg1	spectrometry					1541:1552	liquid chromatography-mass spectrometry	1514:1552	liquid chromatography-mass spectrometry	1514:1552	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	12	80	theme	preferential	2202:2213	arg1	removal					2215:2221	preferential removal	2202:2221	preferential removal of Neu5Ac	2202:2231	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	7	81	theme	CP1	1215:1217	arg1	CAZome					1094:1099	the CAZome	1090:1099	the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1	1090:1217	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	81	theme	CP1	1215:1217	arg1	enzymes					1254:1260	several putative and known enzymes	1227:1260	several putative and known enzymes with activities consistent with the modification of mucin	1227:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	3	82	theme	rich	602:605	arg1	N-					493:494	N-	493:494	N-	493:494	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	3	82	theme	rich	602:605	arg1	source					614:619	a rich carbon source	600:619	a rich carbon source for mucolytic bacteria	600:642	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	9	83	theme	mucin	1563:1567	arg1	monosaccharides					1569:1583	Chicken mucin monosaccharides	1555:1583	Chicken mucin monosaccharides	1555:1583	Chicken mucin monosaccharides included l-fucose (Fuc), d-mannose (Man), d-galactose (Gal), N-acetyl-d-galactosamine (GalNAc), N-acetyl-d-glucosamine (GlcNAc), and Neu5Ac (sialic acid).					
31180129	6	84	theme	intestinal	1030:1039	arg1	layer					1047:1051	the poultry intestinal mucus layer	1018:1051	the poultry intestinal mucus layer	1018:1051	The mechanism by which C. perfringens penetrates the poultry intestinal mucus layer during NE is still unknown.					
31180129	4	85	theme	glycans	679:685	arg1	different					699:707	different	699:707	different	699:707	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	85	theme	glycans	679:685	arg1	composition					649:659	The composition	645:659	The composition of avian O-linked glycans	645:685	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	0	86	theme	Clostridium	87:97	arg1	perfringens					99:109	Clostridium perfringens	87:109	Clostridium perfringens	87:109	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	12	87	with	analysis	2154:2161	arg1	perfringens					2127:2137	C. perfringens	2124:2137	C. perfringens	2124:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	7	88	theme	active	1165:1170	arg1	genome					1158:1163	a genome	1156:1163	a genome active on carbohydrates	1156:1187	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	7	89	theme	mucin	1314:1318	arg1	modification					1298:1309	the modification	1294:1309	the modification of mucin	1294:1318	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	12	90	theme	glycans	2170:2176	arg1	analysis					2154:2161	subsequent analysis	2143:2161	subsequent analysis of the glycans	2143:2176	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	12	90	theme	glycans	2170:2176	arg1	incubation					2081:2090	anaerobic incubation	2071:2090	anaerobic incubation of chicken mucin O-glycans with C. perfringens	2071:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	2	91	theme	small	378:382	arg1	intestine					384:392	the small intestine	374:392	the small intestine	374:392	The ability of C. perfringens to incite NE depends upon its ability to penetrate the protective mucus barrier within the small intestine, which is largely composed of heavily glycosylated proteins called mucins.					
31180129	0	92	theme	broiler	23:29	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	1	93	theme	Clostridium	112:122	arg1	agent					212:216	the principal etiological agent	186:216	the principal etiological agent of necrotic enteritis (NE) in poultry	186:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	93	theme	Clostridium	112:122	arg1	pathogen					169:176	a Gram-positive opportunistic pathogen	139:176	a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry	139:254	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	1	93	theme	Clostridium	112:122	arg1	perfringens					124:134	Clostridium perfringens	112:134	Clostridium perfringens	112:134	Clostridium perfringens is a Gram-positive opportunistic pathogen that is the principal etiological agent of necrotic enteritis (NE) in poultry.					
31180129	8	94	theme	Gallus	1378:1383	arg1	gallus					1385:1390	Gallus gallus domesticus mucus	1378:1407	Gallus gallus domesticus mucus	1378:1407	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	13	95	theme	potential	2309:2317	arg1	enzymes					2359:2365	the mucolytic enzymes	2345:2365	the mucolytic enzymes encoded within its CAZome	2345:2391	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	13	95	theme	potential	2309:2317	arg1	context					2274:2280	the context	2270:2280	the context of the predicted metabolic potential of C. perfringens CP1	2270:2339	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	0	96	theme	small	39:43	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	13	97	theme	mucolytic	2349:2357	arg1	enzymes					2359:2365	the mucolytic enzymes	2345:2365	the mucolytic enzymes encoded within its CAZome	2345:2391	These observations are discussed in the context of the predicted metabolic potential of C. perfringens CP1 and the mucolytic enzymes encoded within its CAZome.					
31180129	3	98	theme	mucolytic	625:633	arg1	bacteria					635:642	mucolytic bacteria	625:642	mucolytic bacteria	625:642	Mucins are decorated by N- and O-linked glycans that serve both as a formidable gel-like barrier against invading pathogens and as a rich carbon source for mucolytic bacteria.					
31180129	8	99	theme	domesticus	1392:1401	arg1	gallus					1385:1390	Gallus gallus domesticus mucus	1378:1407	Gallus gallus domesticus mucus	1378:1407	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	0	100	theme	mucin	56:60	arg1	modification					71:82	broiler chicken small intestinal mucin O-glycan modification	23:82	broiler chicken small intestinal mucin O-glycan modification	23:82	Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens.					
31180129	9	101	theme	sialic	1726:1731	arg1	Neu5Ac					1718:1723	Neu5Ac	1718:1723	Neu5Ac (sialic acid)	1718:1737	Chicken mucin monosaccharides included l-fucose (Fuc), d-mannose (Man), d-galactose (Gal), N-acetyl-d-galactosamine (GalNAc), N-acetyl-d-glucosamine (GlcNAc), and Neu5Ac (sialic acid).					
31180129	9	101	theme	sialic	1726:1731	arg1	acid					1733:1736	sialic acid	1726:1736	sialic acid	1726:1736	Chicken mucin monosaccharides included l-fucose (Fuc), d-mannose (Man), d-galactose (Gal), N-acetyl-d-galactosamine (GalNAc), N-acetyl-d-glucosamine (GlcNAc), and Neu5Ac (sialic acid).					
31180129	7	102	dep	C.	1193:1194	arg1	perfringens					1196:1206	perfringens	1196:1206	perfringens	1196:1206	However, the CAZome (i.e., the total collection of proteins encoded within a genome active on carbohydrates) of C. perfringens strain CP1 encodes several putative and known enzymes with activities consistent with the modification of mucin.					
31180129	4	103	theme	N-acetyl-d-neuraminic	790:810	arg1	acid					812:815	N-acetyl-d-neuraminic acid	790:815	N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid)	790:837	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	12	104	with	incubation	2081:2090	arg1	perfringens					2127:2137	C. perfringens	2124:2137	C. perfringens	2124:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	4	105	from	mucins	714:719	arg1	vertebrates					730:740	other vertebrates	724:740	other vertebrates	724:740	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	105	from	mucins	714:719	arg1	different					699:707	different	699:707	different	699:707	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	4	105	from	mucins	714:719	arg1	composition					649:659	The composition	645:659	The composition of avian O-linked glycans	645:685	The composition of avian O-linked glycans is markedly different from mucins in other vertebrates, being enriched in sulfated monosaccharides and N-acetyl-d-neuraminic acid (Neu5Ac, sialic acid).					
31180129	12	106	theme	anaerobic	2071:2079	arg1	incubation					2081:2090	anaerobic incubation	2071:2090	anaerobic incubation of chicken mucin O-glycans with C. perfringens	2071:2137	Finally, anaerobic incubation of chicken mucin O-glycans with C. perfringens and subsequent analysis of the glycans revealed that there was preferential removal of Neu5Ac.					
31180129	8	107	theme	small	1433:1437	arg1	intestine					1439:1447	the small intestine	1429:1447	the small intestine	1429:1447	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31180129	8	108	from	gallus	1385:1390	arg1	O-glycans					1363:1371	O-glycans	1363:1371	O-glycans from Gallus gallus domesticus mucus	1363:1407	To further investigate this relationship, O-glycans from Gallus gallus domesticus mucus were extracted from the small intestine and characterized using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.					
31558607	8	0	theme	N-glycans	1537:1545	arg1	type					1529:1532	an unusual type	1518:1532	an unusual type of N-glycans important for its processing and secretion	1518:1588	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	8	1	theme	study	1484:1488	arg1	findings					1468:1475	The findings	1464:1475	The findings of our study	1464:1488	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	8	2	contain	carries	1510:1516	arg1	proBDNF					1502:1508	proBDNF	1502:1508	proBDNF	1502:1508	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	8	2	contain	carries	1510:1516	arg2	type					1529:1532	an unusual type	1518:1532	an unusual type of N-glycans important for its processing and secretion	1518:1588	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	1	3	theme	proBDNF	247:253	arg1	precursor					255:263	the proBDNF precursor	243:263	the proBDNF precursor	243:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	2	4	gly	N-glycosylation	397:411	arg2	sequon					413:418	a single N-glycosylation sequon	388:418	a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site	388:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	2	4	gly	N-glycosylation	397:411	arg2	Asn-127					421:427	Asn-127	421:427	Asn-127	421:427	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	4	5	theme	Asn-127	708:714	arg1	mutation					692:699	mutation	692:699	mutation of the Asn-127	692:714	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	2	6	from	located	477:483	arg1	region					507:512	a highly conserved region	488:512	a highly conserved region proximal to the proteolytic site	488:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	9	7	theme	protein	1658:1664	arg1	glycoforms					1666:1675	these protein glycoforms	1652:1675	these protein glycoforms	1652:1675	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	4	8	theme	WT	789:790	arg1	proBDNF					792:798	WT proBDNF	789:798	WT proBDNF	789:798	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	5	9	theme	proBDNF	1028:1034	arg1	maturation					1036:1045	proBDNF maturation	1028:1045	proBDNF maturation	1028:1045	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	2	10	contain	carries	380:386	arg2	sequon					413:418	a single N-glycosylation sequon	388:418	a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site	388:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	2	10	contain	carries	380:386	arg1	ProBDNF					372:378	ProBDNF	372:378	ProBDNF	372:378	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	2	10	contain	carries	380:386	arg2	Asn-127					421:427	Asn-127	421:427	Asn-127	421:427	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	6	11	gly	glycosylated	1132:1143	arg1	proBDNF					1115:1121	secreted proBDNF	1106:1121	secreted proBDNF	1106:1121	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	5	12	theme	furin-proBDNF	989:1001	arg1	interaction					1003:1013	a direct furin-proBDNF interaction	980:1013	effects other than a direct furin-proBDNF interaction	961:1013	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	6	13	theme	rare	1157:1160	arg1	N-glycans					1162:1170	rare N-glycans	1157:1170	rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation	1157:1259	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	7	14	theme	PC12	1367:1370	arg1	cells					1372:1376	PC12 cells	1367:1376	PC12 cells	1367:1376	We and others noted that this type of glycosylation is protein-specific, extends to proBDNF expressed in PC12 cells, and implies the presence of interacting partners that recognize this glycan epitope.					
31558607	6	15	theme	secreted	1106:1113	arg1	proBDNF					1115:1121	secreted proBDNF	1106:1121	secreted proBDNF	1106:1121	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	2	16	theme	proximal	514:521	arg1	region					507:512	a highly conserved region	488:512	a highly conserved region proximal to the proteolytic site	488:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	3	17	theme	proBDNF	561:567	arg1	structure					569:577	the proBDNF structure	557:577	the proBDNF structure	557:577	To study the proBDNF structure and function, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.					
31558607	0	18	theme	neurotrophic	76:87	arg1	proBDNF					97:103	proBDNF	97:103	proBDNF	97:103	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	0	18	theme	neurotrophic	76:87	arg1	factor					89:94	brain-derived neurotrophic factor	62:94	brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures	62:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	2	19	theme	proteolytic	530:540	arg1	site					542:545	the proteolytic site	526:545	the proteolytic site	526:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	3	20	gly	nonglycosylated	631:645	arg1	mutant					653:658	its nonglycosylated N127Q mutant	627:658	its nonglycosylated N127Q mutant	627:658	To study the proBDNF structure and function, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.					
31558607	9	21	theme	different	1680:1688	arg1	cells					1690:1694	different cells	1680:1694	different cells	1680:1694	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	7	22	theme	interacting	1407:1417	arg1	partners					1419:1426	interacting partners	1407:1426	interacting partners that recognize this glycan epitope	1407:1461	We and others noted that this type of glycosylation is protein-specific, extends to proBDNF expressed in PC12 cells, and implies the presence of interacting partners that recognize this glycan epitope.					
31558607	5	23	theme	proBDNF	909:915	arg1	cleavage					917:924	proBDNF cleavage	909:924	proBDNF cleavage by furin	909:933	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	1	24	theme	furin-like	291:300	arg1	proteases					302:310	furin-like proteases	291:310	furin-like proteases	291:310	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	0	25	theme	LacdiNAc	124:131	arg1	structures					133:142	sulfated LacdiNAc structures	115:142	sulfated LacdiNAc structures	115:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	7	26	theme	glycan	1448:1453	arg1	epitope					1455:1461	this glycan epitope	1443:1461	this glycan epitope	1443:1461	We and others noted that this type of glycosylation is protein-specific, extends to proBDNF expressed in PC12 cells, and implies the presence of interacting partners that recognize this glycan epitope.					
31558607	5	27	theme	N-glycan	869:876	arg1	Absence					854:860	Absence	854:860	Absence of the N-glycan	854:876	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	0	28	theme	sulfated	115:122	arg1	structures					133:142	sulfated LacdiNAc structures	115:142	sulfated LacdiNAc structures	115:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	6	29	theme	terminal	1242:1249	arg1	sulfation					1251:1259	terminal sulfation	1242:1259	terminal sulfation	1242:1259	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	5	30	dep	other	969:973	arg1	than					975:978	than	975:978	than	975:978	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	1	31	theme	proteolytic	202:212	arg1	cleavage					214:221	proteolytic cleavage	202:221	proteolytic cleavage of a prodomain from the proBDNF precursor	202:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	5	32	theme	direct	982:987	arg1	interaction					1003:1013	a direct furin-proBDNF interaction	980:1013	effects other than a direct furin-proBDNF interaction	961:1013	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	1	33	link	Brain-derived	145:157	arg1	BDNF					180:183	BDNF	180:183	BDNF	180:183	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	33	link	Brain-derived	145:157	arg1	factor					172:177	Brain-derived neurotrophic factor	145:177	Brain-derived neurotrophic factor (BDNF)	145:184	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	7	34	theme	glycosylation	1300:1312	arg1	type					1292:1295	this type	1287:1295	this type of glycosylation	1287:1312	We and others noted that this type of glycosylation is protein-specific, extends to proBDNF expressed in PC12 cells, and implies the presence of interacting partners that recognize this glycan epitope.					
31558607	2	35	theme	conserved	497:505	arg1	region					507:512	a highly conserved region	488:512	a highly conserved region proximal to the proteolytic site	488:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	1	36	from	precursor	255:263	arg1	cleavage					214:221	proteolytic cleavage	202:221	proteolytic cleavage of a prodomain from the proBDNF precursor	202:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	36	from	precursor	255:263	arg1	prodomain					228:236	a prodomain	226:236	a prodomain from the proBDNF precursor	226:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	37	from	cleavage	214:221	arg1	precursor					255:263	the proBDNF precursor	243:263	the proBDNF precursor	243:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	0	38	theme	precursor	49:57	arg1	secretion					32:40	secretion	32:40	secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures	32:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	5	39	theme	cleavage	917:924	arg1	kinetics					897:904	the kinetics	893:904	the kinetics of proBDNF cleavage by furin	893:933	Absence of the N-glycan did not affect the kinetics of proBDNF cleavage by furin in vitro, indicating that effects other than a direct furin-proBDNF interaction may regulate proBDNF maturation.					
31558607	3	40	theme	HEK293F	663:669	arg1	cells					671:675	HEK293F cells	663:675	HEK293F cells	663:675	To study the proBDNF structure and function, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.					
31558607	7	41	theme	partners	1419:1426	arg1	presence					1395:1402	the presence	1391:1402	the presence of interacting partners that recognize this glycan epitope	1391:1461	We and others noted that this type of glycosylation is protein-specific, extends to proBDNF expressed in PC12 cells, and implies the presence of interacting partners that recognize this glycan epitope.					
31558607	0	42	contain	carrying	106:113	arg1	proBDNF					97:103	proBDNF	97:103	proBDNF	97:103	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	0	42	contain	carrying	106:113	arg1	factor					89:94	brain-derived neurotrophic factor	62:94	brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures	62:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	0	42	contain	carrying	106:113	arg2	structures					133:142	sulfated LacdiNAc structures	115:142	sulfated LacdiNAc structures	115:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	2	43	from	region	507:512	arg1	located					477:483	located	477:483	located	477:483	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	8	44	theme	important	1547:1555	arg1	type					1529:1532	an unusual type	1518:1532	an unusual type of N-glycans important for its processing and secretion	1518:1588	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	8	45	theme	unusual	1521:1527	arg1	type					1529:1532	an unusual type	1518:1532	an unusual type of N-glycans important for its processing and secretion	1518:1588	The findings of our study reveal that proBDNF carries an unusual type of N-glycans important for its processing and secretion.					
31558607	9	46	theme	glycoforms	1666:1675	arg1	studies					1641:1647	functional studies	1630:1647	functional studies of these protein glycoforms in different cells and tissues	1630:1706	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	0	47	link	brain-derived	62:74	arg1	proBDNF					97:103	proBDNF	97:103	proBDNF	97:103	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	0	47	link	brain-derived	62:74	arg1	factor					89:94	brain-derived neurotrophic factor	62:94	brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures	62:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	4	48	theme	intracellular	725:737	arg1	effect					768:773	an effect	765:773	an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation	765:851	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	4	48	theme	intracellular	725:737	arg1	maturation					739:748	intracellular maturation	725:748	intracellular maturation	725:748	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	1	49	theme	Brain-derived	145:157	arg1	BDNF					180:183	BDNF	180:183	BDNF	180:183	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	49	theme	Brain-derived	145:157	arg1	factor					172:177	Brain-derived neurotrophic factor	145:177	Brain-derived neurotrophic factor (BDNF)	145:184	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	3	50	theme	N127Q	647:651	arg1	mutant					653:658	its nonglycosylated N127Q mutant	627:658	its nonglycosylated N127Q mutant	627:658	To study the proBDNF structure and function, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.					
31558607	6	51	theme	optimized	1057:1065	arg1	workflow					1076:1083	an optimized LC-MS/MS workflow	1054:1083	an optimized LC-MS/MS workflow	1054:1083	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	9	52	theme	functional	1630:1639	arg1	studies					1641:1647	functional studies	1630:1647	functional studies of these protein glycoforms in different cells and tissues	1630:1706	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	6	53	theme	LC-MS/MS	1067:1074	arg1	workflow					1076:1083	an optimized LC-MS/MS workflow	1054:1083	an optimized LC-MS/MS workflow	1054:1083	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	9	54	theme	new	1608:1610	arg1	opportunities					1612:1624	new opportunities	1608:1624	new opportunities for functional studies of these protein glycoforms in different cells and tissues	1608:1706	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	4	55	theme	tunicamycin-induced	803:821	arg1	inhibition					823:832	tunicamycin-induced inhibition	803:832	tunicamycin-induced inhibition of N-glycosylation	803:851	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	1	56	theme	neurotrophic	159:170	arg1	BDNF					180:183	BDNF	180:183	BDNF	180:183	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	56	theme	neurotrophic	159:170	arg1	factor					172:177	Brain-derived neurotrophic factor	145:177	Brain-derived neurotrophic factor (BDNF)	145:184	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	1	57	theme	prodomain	228:236	arg1	cleavage					214:221	proteolytic cleavage	202:221	proteolytic cleavage of a prodomain from the proBDNF precursor	202:263	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	0	58	theme	brain-derived	62:74	arg1	proBDNF					97:103	proBDNF	97:103	proBDNF	97:103	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	0	58	theme	brain-derived	62:74	arg1	factor					89:94	brain-derived neurotrophic factor	62:94	brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures	62:142	N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures.					
31558607	6	59	contain	carries	1149:1155	arg2	N-glycans					1162:1170	rare N-glycans	1157:1170	rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation	1157:1259	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	6	59	contain	carries	1149:1155	arg1	proBDNF					1115:1121	secreted proBDNF	1106:1121	secreted proBDNF	1106:1121	Using an optimized LC-MS/MS workflow, we demonstrate that secreted proBDNF is fully glycosylated and carries rare N-glycans terminated by GalNAcβ1-4GlcNAcβ1-R (LacdiNAc) extensively modified by terminal sulfation.					
31558607	4	60	theme	N-glycosylation	837:851	arg1	inhibition					823:832	tunicamycin-induced inhibition	803:832	tunicamycin-induced inhibition of N-glycosylation	803:851	We found that mutation of the Asn-127 prevents intracellular maturation and secretion, an effect reproduced in WT proBDNF by tunicamycin-induced inhibition of N-glycosylation.					
31558607	2	61	theme	N-glycosylation	397:411	arg1	Asn-127					421:427	Asn-127	421:427	Asn-127	421:427	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	2	61	theme	N-glycosylation	397:411	arg1	sequon					413:418	a single N-glycosylation sequon	388:418	a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site	388:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	9	62	from	studies	1641:1647	arg1	tissues					1700:1706	tissues	1700:1706	tissues	1700:1706	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	9	62	from	studies	1641:1647	arg1	cells					1690:1694	different cells	1680:1694	different cells	1680:1694	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	9	63	gly	glycoforms	1666:1675	arg1	protein					1658:1664	these protein glycoforms	1652:1675	these protein glycoforms	1652:1675	Our results open new opportunities for functional studies of these protein glycoforms in different cells and tissues.					
31558607	1	64	theme	matrix	345:350	arg1	metalloproteinases					352:369	matrix metalloproteinases	345:369	matrix metalloproteinases	345:369	Brain-derived neurotrophic factor (BDNF) is generated by proteolytic cleavage of a prodomain from the proBDNF precursor either intracellularly by furin-like proteases or extracellularly by plasmin or matrix metalloproteinases.					
31558607	3	65	theme	nonglycosylated	631:645	arg1	mutant					653:658	its nonglycosylated N127Q mutant	627:658	its nonglycosylated N127Q mutant	627:658	To study the proBDNF structure and function, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.					
31558607	2	66	theme	single	390:395	arg1	Asn-127					421:427	Asn-127	421:427	Asn-127	421:427	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31558607	2	66	theme	single	390:395	arg1	sequon					413:418	a single N-glycosylation sequon	388:418	a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site	388:545	ProBDNF carries a single N-glycosylation sequon (Asn-127) that remains virtually unstudied despite being located in a highly conserved region proximal to the proteolytic site.					
31545058	0	0	theme	Oligosaccharide	104:118	arg1	Assemblies					120:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	1	1	theme	glycosides	180:189	arg1	type					156:159	a new type	150:159	a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature	150:295	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	0	2	theme	One-Pot	96:102	arg1	Assemblies					120:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	0	3	from	Glycosides	16:25	arg1	Assemblies					120:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	3	4	theme	2-catalyzed	528:538	arg1	conditions					540:549	the Hg(NTf2)2-catalyzed conditions	516:549	the Hg(NTf2)2-catalyzed conditions	516:549	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	3	5	theme	sequential	556:565	arg1	activation					567:576	the sequential activation	552:576	the sequential activation of different types of donors	552:605	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	3	6	theme	types	591:595	arg1	activation					567:576	the sequential activation	552:576	the sequential activation of different types of donors	552:605	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	0	7	from	Application	65:75	arg1	Assemblies					120:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	2	8	theme	common	358:363	arg1	manipulations					389:401	common protection/deprotection manipulations	358:401	common protection/deprotection manipulations	358:401	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	1	9	theme	PPh3AuCl/AgNTf2	260:274	arg1	amounts					226:232	catalytic amounts	216:232	catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2	216:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	9	theme	PPh3AuCl/AgNTf2	260:274	arg1	NTf2					250:253	Hg(NTf2)2	247:255	Hg(NTf2)2	247:255	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	9	theme	PPh3AuCl/AgNTf2	260:274	arg1	PPh3AuCl/AgNTf2					260:274	PPh3AuCl/AgNTf2	260:274	PPh3AuCl/AgNTf2	260:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	9	theme	PPh3AuCl/AgNTf2	260:274	arg1	promoter					237:244	promoter	237:244	promoter	237:244	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	2	10	theme	various	471:477	arg1	bonds					490:494	various glycosidic bonds	471:494	various glycosidic bonds	471:494	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	11	theme	enynyl	414:419	arg1	donors					421:426	the enynyl donors	410:426	the enynyl donors	410:426	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	12	theme	glycosidic	479:488	arg1	bonds					490:494	various glycosidic bonds	471:494	various glycosidic bonds	471:494	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	13	theme	activation	307:316	arg1	conditions					318:327	The mild activation conditions	298:327	The mild activation conditions	298:327	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	3	14	dep	achieved	611:618	arg1	based					621:625	based	621:625	based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy	621:731	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	0	15	theme	n-Pentenyl-Type	0:14	arg1	Glycosides					16:25	n-Pentenyl-Type Glycosides	0:25	n-Pentenyl-Type Glycosides for Catalytic Glycosylation	0:53	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	2	16	theme	mild	302:305	arg1	conditions					318:327	The mild activation conditions	298:327	The mild activation conditions	298:327	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	0	17	theme	Catalytic	31:39	arg1	Glycosylation					41:53	Catalytic Glycosylation	31:53	Catalytic Glycosylation	31:53	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	1	18	theme	catalytic	216:224	arg1	amounts					226:232	catalytic amounts	216:232	catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2	216:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	18	theme	catalytic	216:224	arg1	NTf2					250:253	Hg(NTf2)2	247:255	Hg(NTf2)2	247:255	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	18	theme	catalytic	216:224	arg1	PPh3AuCl/AgNTf2					260:274	PPh3AuCl/AgNTf2	260:274	PPh3AuCl/AgNTf2	260:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	18	theme	catalytic	216:224	arg1	promoter					237:244	promoter	237:244	promoter	237:244	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	19	theme	room	280:283	arg1	temperature					285:295	room temperature	280:295	room temperature	280:295	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	3	20	theme	different	581:589	arg1	types					591:595	different types	581:595	different types of donors	581:605	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	2	21	theme	broad	436:440	arg1	applications					442:453	broad applications	436:453	broad applications	436:453	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	3	22	theme	NTf2	523:526	arg1	conditions					540:549	the Hg(NTf2)2-catalyzed conditions	516:549	the Hg(NTf2)2-catalyzed conditions	516:549	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	1	23	theme	promoter	237:244	arg1	amounts					226:232	catalytic amounts	216:232	catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2	216:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	23	theme	promoter	237:244	arg1	NTf2					250:253	Hg(NTf2)2	247:255	Hg(NTf2)2	247:255	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	23	theme	promoter	237:244	arg1	PPh3AuCl/AgNTf2					260:274	PPh3AuCl/AgNTf2	260:274	PPh3AuCl/AgNTf2	260:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	23	theme	promoter	237:244	arg1	promoter					237:244	promoter	237:244	promoter	237:244	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	3	24	theme	donors	600:605	arg1	types					591:595	different types	581:595	different types of donors	581:605	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	3	25	theme	Hg	520:521	arg1	conditions					540:549	the Hg(NTf2)2-catalyzed conditions	516:549	the Hg(NTf2)2-catalyzed conditions	516:549	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	3	26	theme	developed	690:698	arg1	strategy					724:731	the newly developed single-catalyst one-pot strategy	680:731	the newly developed single-catalyst one-pot strategy	680:731	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	3	27	theme	single-catalyst	700:714	arg1	strategy					724:731	the newly developed single-catalyst one-pot strategy	680:731	the newly developed single-catalyst one-pot strategy	680:731	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	2	28	theme	outstanding	333:343	arg1	stability					345:353	outstanding stability	333:353	outstanding stability	333:353	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	3	29	theme	one-pot	716:722	arg1	strategy					724:731	the newly developed single-catalyst one-pot strategy	680:731	the newly developed single-catalyst one-pot strategy	680:731	Furthermore, under the Hg(NTf2)2-catalyzed conditions, the sequential activation of different types of donors was achieved, based on which a gentiotetrasaccharide was synthesized via the newly developed single-catalyst one-pot strategy.					
31545058	2	30	contain	have	431:434	arg2	applications					442:453	broad applications	436:453	broad applications	436:453	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	30	contain	have	431:434	arg1	donors					421:426	the enynyl donors	410:426	the enynyl donors	410:426	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	1	31	theme	new	152:154	arg1	type					156:159	a new type	150:159	a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature	150:295	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	32	theme	n-pentenyl-type	164:178	arg1	glycosides					180:189	n-pentenyl-type glycosides	164:189	n-pentenyl-type glycosides	164:189	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	33	theme	Hg	247:248	arg1	NTf2					250:253	Hg(NTf2)2	247:255	Hg(NTf2)2	247:255	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	0	34	theme	Single-Catalyst	80:94	arg1	Assemblies					120:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	Single-Catalyst One-Pot Oligosaccharide Assemblies	80:129	n-Pentenyl-Type Glycosides for Catalytic Glycosylation and Their Application in Single-Catalyst One-Pot Oligosaccharide Assemblies.					
31545058	2	35	theme	manipulations	389:401	arg1	conditions					318:327	The mild activation conditions	298:327	The mild activation conditions	298:327	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	35	theme	manipulations	389:401	arg1	stability					345:353	outstanding stability	333:353	outstanding stability	333:353	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	2	36	theme	protection/deprotection	365:387	arg1	manipulations					389:401	common protection/deprotection manipulations	358:401	common protection/deprotection manipulations	358:401	The mild activation conditions and outstanding stability of common protection/deprotection manipulations enable the enynyl donors to have broad applications in constructing various glycosidic bonds.					
31545058	1	37	theme	NTf2	250:253	arg1	amounts					226:232	catalytic amounts	216:232	catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2	216:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	37	theme	NTf2	250:253	arg1	NTf2					250:253	Hg(NTf2)2	247:255	Hg(NTf2)2	247:255	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	37	theme	NTf2	250:253	arg1	PPh3AuCl/AgNTf2					260:274	PPh3AuCl/AgNTf2	260:274	PPh3AuCl/AgNTf2	260:274	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
31545058	1	37	theme	NTf2	250:253	arg1	promoter					237:244	promoter	237:244	promoter	237:244	We have developed a new type of n-pentenyl-type glycosides that can be activated by catalytic amounts of promoter, Hg(NTf2)2 or PPh3AuCl/AgNTf2, at room temperature.					
29443078	4	0	theme	Man5GlcNAc2	943:953	arg1	mixture					917:923	the mixture	913:923	the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array	913:1039	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	3	1	theme	ACG	693:695	arg1	slide					698:702	an aluminum oxide-coated glass (ACG) slide	661:702	an aluminum oxide-coated glass (ACG) slide	661:702	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	4	2	theme	effective	1074:1082	arg1	design					1094:1099	the effective immunogen design	1070:1099	the effective immunogen design for HIV vaccine development	1070:1127	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	3	theme	complex	997:1003	arg1	N-glycan					1010:1017	2,6-di-sialylated bi-antennary complex type N-glycan	966:1017	2,6-di-sialylated bi-antennary complex type N-glycan (SCT)	966:1023	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	3	theme	complex	997:1003	arg1	SCT					1020:1022	SCT	1020:1022	SCT	1020:1022	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	3	4	theme	oxide-coated	673:684	arg1	slide					698:702	an aluminum oxide-coated glass (ACG) slide	661:702	an aluminum oxide-coated glass (ACG) slide	661:702	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	2	5	theme	β-mannoside	588:598	arg1	linkage					600:606	a crucial β-mannoside linkage	578:606	a crucial β-mannoside linkage	578:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	5	6	theme	powerful	1168:1175	arg1	tool					1177:1180	a powerful tool	1166:1180	a powerful tool to study other HIV antibodies for hetero-ligand binding behavior	1166:1245	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	3	7	theme	glass	686:690	arg1	slide					698:702	an aluminum oxide-coated glass (ACG) slide	661:702	an aluminum oxide-coated glass (ACG) slide	661:702	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	2	8	theme	crucial	580:586	arg1	linkage					600:606	a crucial β-mannoside linkage	578:606	a crucial β-mannoside linkage	578:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	9	dep	began	340:344	arg1	followed					432:439	followed	432:439	followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	432:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	10	theme	N-glycan	1010:1017	arg1	mixture					917:923	the mixture	913:923	the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array	913:1039	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	3	11	theme	HIV	791:793	arg1	antibody					795:802	an HIV antibody	788:802	an HIV antibody	788:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	1	12	dep	oligosaccharides	177:192	arg1	estimated					195:203	estimated	195:203	estimated to exceed 20,000 structures	195:231	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	5	13	theme	hetero-ligand	1216:1228	arg1	behavior					1238:1245	hetero-ligand binding behavior	1216:1245	hetero-ligand binding behavior	1216:1245	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	4	14	theme	new	1050:1052	arg1	avenue					1054:1059	a new avenue	1048:1059	a new avenue to guide the effective immunogen design for HIV vaccine development	1048:1127	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	5	15	theme	other	1191:1195	arg1	antibodies					1201:1210	other HIV antibodies	1191:1210	other HIV antibodies for hetero-ligand binding behavior	1191:1245	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	1	16	theme	wide	154:157	arg1	range					159:163	a wide range	152:163	a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	152:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	2	17	theme	6-O	501:503	arg1	positions					505:513	the 3-O and 6-O positions	489:513	the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	489:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	1	18	link	N-linked	168:175	arg1	oligosaccharides					177:192	N-linked oligosaccharides	168:192	N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	168:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	3	19	with	interaction	771:781	arg1	antibody					795:802	an HIV antibody	788:802	an HIV antibody	788:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	1	20	theme	range	159:163	arg1	preparation					137:147	the rapid preparation	127:147	the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	127:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	3	21	theme	slide	698:702	arg1	surface					650:656	the surface	646:656	the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody	646:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	4	22	theme	binding	824:830	arg1	behavior					832:839	the binding behavior	820:839	the binding behavior	820:839	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	2	23	theme	desired	297:303	arg1	diversity					316:324	the desired structural diversity	293:324	the desired structural diversity	293:324	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	24	theme	isolated	852:859	arg1	HIV-1					861:865	a newly isolated HIV-1	844:865	a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9	844:907	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	0	25	theme	Chemo-enzymatic	0:14	arg1	Synthesis					16:24	Chemo-enzymatic Synthesis	0:24	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling	0:86	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling.					
29443078	3	26	theme	mixed	725:729	arg1	array					731:735	a covalent mixed array	714:735	a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody	714:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	1	27	theme	N-linked	168:175	arg1	oligosaccharides					177:192	N-linked oligosaccharides	168:192	N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	168:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	0	28	theme	N-glycans	29:37	arg1	Synthesis					16:24	Chemo-enzymatic Synthesis	0:24	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling	0:86	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling.					
29443078	1	29	theme	human	261:265	arg1	glycoproteins					267:279	human glycoproteins	261:279	human glycoproteins	261:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	4	30	theme	ACG	1031:1033	arg1	array					1035:1039	an ACG array	1028:1039	an ACG array	1028:1039	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	2	31	theme	fluoride	414:421	arg1	modules					423:429	oligosaccharyl fluoride modules	399:429	oligosaccharyl fluoride modules	399:429	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	32	theme	modules	423:429	arg1	kinds					390:394	three kinds	384:394	three kinds of oligosaccharyl fluoride modules	384:429	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	5	33	theme	binding	1230:1236	arg1	behavior					1238:1245	hetero-ligand binding behavior	1216:1245	hetero-ligand binding behavior	1216:1245	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	4	34	theme	bi-antennary	984:995	arg1	N-glycan					1010:1017	2,6-di-sialylated bi-antennary complex type N-glycan	966:1017	2,6-di-sialylated bi-antennary complex type N-glycan (SCT)	966:1023	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	34	theme	bi-antennary	984:995	arg1	SCT					1020:1022	SCT	1020:1022	SCT	1020:1022	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	2	35	theme	oligosaccharyl	399:412	arg1	modules					423:429	oligosaccharyl fluoride modules	399:429	oligosaccharyl fluoride modules	399:429	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	0	36	theme	Array	43:47	arg1	Development					49:59	Array Development	43:59	Array Development	43:59	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling.					
29443078	3	37	theme	covalent	716:723	arg1	array					731:735	a covalent mixed array	714:735	a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody	714:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	2	38	theme	residue	530:536	arg1	positions					505:513	the 3-O and 6-O positions	489:513	the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	489:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	5	39	theme	ACG	1147:1149	arg1	array					1151:1155	our ACG array	1143:1155	our ACG array	1143:1155	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	2	40	contain	having	571:576	arg2	linkage					600:606	a crucial β-mannoside linkage	578:606	a crucial β-mannoside linkage	578:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	40	contain	having	571:576	arg1	trisaccharide					557:569	the common core trisaccharide	541:569	the common core trisaccharide having a crucial β-mannoside linkage	541:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	41	theme	α-selective	459:469	arg1	glycosylations					471:484	their stepwise α-selective glycosylations	444:484	their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	444:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	42	theme	mannose	522:528	arg1	trisaccharide					557:569	the common core trisaccharide	541:569	the common core trisaccharide having a crucial β-mannoside linkage	541:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	42	theme	mannose	522:528	arg1	residue					530:536	the mannose residue	518:536	the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	518:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	43	gly	glycosylations	471:484	arg2	positions					505:513	the 3-O and 6-O positions	489:513	the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	489:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	44	theme	kinds	390:394	arg1	synthesis					371:379	the chemo-enzymatic synthesis	351:379	the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules	351:429	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	45	theme	stepwise	450:457	arg1	glycosylations					471:484	their stepwise α-selective glycosylations	444:484	their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	444:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	46	theme	chemo-enzymatic	355:369	arg1	synthesis					371:379	the chemo-enzymatic synthesis	351:379	the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules	351:429	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	47	theme	HIV-1	861:865	arg1	behavior					832:839	the binding behavior	820:839	the binding behavior	820:839	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	2	48	theme	3-O	493:495	arg1	positions					505:513	the 3-O and 6-O positions	489:513	the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	489:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	49	gly	2,6-di-sialylated	966:982	arg1	N-glycan					1010:1017	2,6-di-sialylated bi-antennary complex type N-glycan	966:1017	2,6-di-sialylated bi-antennary complex type N-glycan (SCT)	966:1023	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	49	gly	2,6-di-sialylated	966:982	arg1	SCT					1020:1022	SCT	1020:1022	SCT	1020:1022	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	1	50	theme	efficient	109:117	arg1	way					119:121	a highly efficient way	100:121	a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	100:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	4	51	theme	HIV	1105:1107	arg1	development					1117:1127	HIV vaccine development	1105:1127	HIV vaccine development	1105:1127	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	2	52	theme	trisaccharide	557:569	arg1	trisaccharide					557:569	the common core trisaccharide	541:569	the common core trisaccharide having a crucial β-mannoside linkage	541:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	52	theme	trisaccharide	557:569	arg1	residue					530:536	the mannose residue	518:536	the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	518:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	53	from	positions	505:513	arg1	glycosylations					471:484	their stepwise α-selective glycosylations	444:484	their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage	444:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	54	theme	vaccine	1109:1115	arg1	development					1117:1127	HIV vaccine development	1105:1127	HIV vaccine development	1105:1127	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	3	55	attach	attached	620:627	arg2	We					609:610	We	609:610	We	609:610	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	3	55	attach	attached	620:627	arg1	surface					650:656	the surface	646:656	the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody	646:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	2	56	theme	core	552:555	arg1	trisaccharide					557:569	the common core trisaccharide	541:569	the common core trisaccharide having a crucial β-mannoside linkage	541:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	3	57	theme	aluminum	664:671	arg1	slide					698:702	an aluminum oxide-coated glass (ACG) slide	661:702	an aluminum oxide-coated glass (ACG) slide	661:702	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	1	58	gly	glycoproteins	267:279	arg1	glycoproteins					267:279	human glycoproteins	261:279	human glycoproteins	261:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	2	59	theme	common	545:550	arg1	trisaccharide					557:569	the common core trisaccharide	541:569	the common core trisaccharide having a crucial β-mannoside linkage	541:606	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	2	60	theme	structural	305:314	arg1	diversity					316:324	the desired structural diversity	293:324	the desired structural diversity	293:324	To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three kinds of oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at the 3-O and 6-O positions of the mannose residue of the common core trisaccharide having a crucial β-mannoside linkage.					
29443078	4	61	theme	2,6-di-sialylated	966:982	arg1	N-glycan					1010:1017	2,6-di-sialylated bi-antennary complex type N-glycan	966:1017	2,6-di-sialylated bi-antennary complex type N-glycan (SCT)	966:1023	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	61	theme	2,6-di-sialylated	966:982	arg1	SCT					1020:1022	SCT	1020:1022	SCT	1020:1022	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	1	62	theme	20,000	215:220	arg1	structures					222:231	20,000 structures	215:231	20,000 structures	215:231	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	0	63	theme	Antibody	69:76	arg1	Profiling					78:86	HIV Antibody Profiling	65:86	HIV Antibody Profiling	65:86	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling.					
29443078	1	64	located	found	252:256	arg1	glycoproteins					267:279	human glycoproteins	261:279	human glycoproteins	261:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	1	64	located	found	252:256	arg2	oligosaccharides					177:192	N-linked oligosaccharides	168:192	N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	168:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	1	65	theme	oligosaccharides	177:192	arg1	range					159:163	a wide range	152:163	a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	152:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	1	66	theme	rapid	131:135	arg1	preparation					137:147	the rapid preparation	127:147	the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins	127:279	We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins.					
29443078	5	67	theme	HIV	1197:1199	arg1	antibodies					1201:1210	other HIV antibodies	1191:1210	other HIV antibodies for hetero-ligand binding behavior	1191:1245	In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior.					
29443078	3	68	theme	hetero-ligand	757:769	arg1	interaction					771:781	hetero-ligand interaction	757:781	hetero-ligand interaction with an HIV antibody	757:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
29443078	0	69	theme	HIV	65:67	arg1	Profiling					78:86	HIV Antibody Profiling	65:86	HIV Antibody Profiling	65:86	Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling.					
29443078	4	70	theme	spaced	936:941	arg1	Man5					956:959	Man5	956:959	Man5	956:959	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	70	theme	spaced	936:941	arg1	Man5GlcNAc2					943:953	closely spaced Man5GlcNAc2	928:953	closely spaced Man5GlcNAc2 (Man5)	928:960	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	71	theme	immunogen	1084:1092	arg1	design					1094:1099	the effective immunogen design	1070:1099	the effective immunogen design for HIV vaccine development	1070:1127	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	72	from	mixture	917:923	arg1	array					1035:1039	an ACG array	1028:1039	an ACG array	1028:1039	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	73	theme	type	1005:1008	arg1	N-glycan					1010:1017	2,6-di-sialylated bi-antennary complex type N-glycan	966:1017	2,6-di-sialylated bi-antennary complex type N-glycan (SCT)	966:1023	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	4	73	theme	type	1005:1008	arg1	SCT					1020:1022	SCT	1020:1022	SCT	1020:1022	In particular, the binding behavior of a newly isolated HIV-1 broadly neutralizing antibody (bNAb), PG9, to the mixture of closely spaced Man5GlcNAc2 (Man5) and 2,6-di-sialylated bi-antennary complex type N-glycan (SCT) on an ACG array, opens a new avenue to guide the effective immunogen design for HIV vaccine development.					
29443078	3	74	theme	interaction	771:781	arg1	analysis					745:752	the analysis	741:752	the analysis of hetero-ligand interaction with an HIV antibody	741:802	We further attached the N-glycans to the surface of an aluminum oxide-coated glass (ACG) slide to create a covalent mixed array for the analysis of hetero-ligand interaction with an HIV antibody.					
31208374	6	0	theme	G	942:942	arg1	plate					949:953	protein G spin plate	934:953	protein G spin plate	934:953	IgG was purified by affinity chromatography using protein G spin plate and further subjected to glycoblotting for glycan release.					
31208374	13	1	gly	glycoproteins	2390:2402	arg1	glycoproteins					2390:2402	the intact glycoproteins	2379:2402	the intact glycoproteins using a glycoprteomics approach	2379:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	2	2	theme	breast	363:368	arg1	cancer					370:375	breast cancer	363:375	breast cancer (BC)	363:380	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	2	2	theme	breast	363:368	arg1	BC					378:379	BC	378:379	BC	378:379	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	1	3	theme	diseases	260:267	arg1	range					251:255	a wide range	244:255	a wide range of diseases including cancer	244:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	1	3	theme	diseases	260:267	arg1	cancer					279:284	cancer	279:284	cancer	279:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	3	4	theme	fraction	523:530	arg1	N-glycome					532:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	6	5	theme	affinity	904:911	arg1	chromatography					913:926	affinity chromatography	904:926	affinity chromatography using protein G spin plate	904:953	IgG was purified by affinity chromatography using protein G spin plate and further subjected to glycoblotting for glycan release.					
31208374	8	6	theme	type	1261:1264	arg1	N-glycans					1266:1274	the NC, 17 complex type N-glycans	1242:1274	N-glycans	1266:1274	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	8	7	theme	patients	1221:1228	arg1	sera					1206:1209	the sera	1202:1209	the sera of all BC patients compared to the NC, 17 complex type N-glycans	1202:1274	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	4	8	theme	glycoblotting-based	673:691	arg1	purification					700:711	glycoblotting-based glycan purification	673:711	glycoblotting-based glycan purification	673:711	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	10	9	from	abundance	1635:1643	arg1	patients					1651:1658	BC patients	1648:1658	BC patients	1648:1658	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	11	10	theme	black	2041:2045	arg1	population					2047:2056	black population	2041:2056	black population	2041:2056	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	9	11	theme	sialylated	1463:1472	arg1	structures					1474:1483	sialylated structures	1463:1483	sialylated structures	1463:1483	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	5	12	dep	stages	793:798	arg1	controls					816:823	normal controls	809:823	normal controls (NC)	809:828	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	5	12	dep	stages	793:798	arg1	NC					826:827	NC	826:827	NC	826:827	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	5	12	dep	stages	793:798	arg1	I-IV					800:803	I-IV	800:803	I-IV	800:803	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	8	13	theme	expression	1292:1301	arg1	abundance					1303:1311	profound expression abundance	1283:1311	profound expression abundance	1283:1311	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	0	14	theme	Ethiopian	112:120	arg1	women					122:126	Ethiopian women	112:126	Ethiopian women	112:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	3	15	theme	purified	491:498	arg1	N-glycome					532:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	5	16	theme	Ethiopian	850:858	arg1	women					860:864	Ethiopian women	850:864	Ethiopian women	850:864	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	8	17	theme	NC	1246:1247	arg1	N-glycans					1266:1274	the NC, 17 complex type N-glycans	1242:1274	N-glycans	1266:1274	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	4	18	theme	based	731:735	arg1	analysis					750:757	MALDI-TOF/MS based quantitative analysis	718:757	MALDI-TOF/MS based quantitative analysis	718:757	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	4	19	theme	glycomics	639:647	arg1	approach					649:656	a comprehensive glycomics approach	623:656	a comprehensive glycomics approach	623:656	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	7	20	theme	various	1088:1094	arg1	bioinformatics					1096:1109	various bioinformatics	1088:1109	various bioinformatics	1088:1109	Mass spectral data were further processed and evaluated rigorously, using various bioinformatics and statistical tools.					
31208374	8	21	dep	stage	1366:1370	arg1	I					1373:1373	I	1373:1373	I	1373:1373	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	8	21	dep	stage	1366:1370	arg1	II					1379:1380	II	1379:1380	II	1379:1380	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	4	22	theme	quantitative	737:748	arg1	analysis					750:757	MALDI-TOF/MS based quantitative analysis	718:757	MALDI-TOF/MS based quantitative analysis	718:757	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	3	23	theme	glycan	581:586	arg1	markers					588:594	non-invasive glycan markers	568:594	non-invasive glycan markers of BC	568:600	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	2	24	dep	patient	328:334	arg1	diagnosis					336:344	diagnosis	336:344	diagnosis	336:344	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	7	25	theme	Mass	1014:1017	arg1	data					1028:1031	Mass spectral data	1014:1031	Mass spectral data	1014:1031	Mass spectral data were further processed and evaluated rigorously, using various bioinformatics and statistical tools.					
31208374	1	26	theme	BACKGROUND	129:138	arg1	Alterations					140:150	BACKGROUND Alterations	129:150	BACKGROUND Alterations in protein glycosylation patterns	129:184	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	11	27	theme	tumor	1947:1951	arg1	cells					1953:1957	tumor cells	1947:1957	tumor cells	1947:1957	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	2	28	from	improvements	312:323	arg1	survival					350:357	survival	350:357	survival	350:357	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	2	28	from	improvements	312:323	arg1	patient					328:334	patient	328:334	patient	328:334	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	4	29	theme	MALDI-TOF/MS	718:729	arg1	analysis					750:757	MALDI-TOF/MS based quantitative analysis	718:757	MALDI-TOF/MS based quantitative analysis	718:757	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	0	30	theme	early	76:80	arg1	detection					82:90	early detection	76:90	early detection of breast cancer in Ethiopian women	76:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	12	31	theme	whole	2133:2137	arg1	serum					2139:2143	whole serum	2133:2143	whole serum	2133:2143	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	1	32	theme	glycosylation	163:175	arg1	patterns					177:184	protein glycosylation patterns	155:184	protein glycosylation patterns	155:184	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	12	33	theme	stage	2319:2323	arg1	patients					2325:2332	early stage patients	2313:2332	early stage patients	2313:2332	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	11	34	theme	BC	2018:2019	arg1	nature					2008:2013	the aggressive nature	1993:2013	the aggressive nature of BC commonly noticed in black population	1993:2056	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	1	35	from	Alterations	140:150	arg1	patterns					177:184	protein glycosylation patterns	155:184	protein glycosylation patterns	155:184	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	12	36	dep	CONCLUSIONS	2059:2069	arg1	revealed					2224:2231	revealed	2224:2231	revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients	2224:2332	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	12	36	dep	CONCLUSIONS	2059:2069	arg1	addressed					2099:2107	addressed	2099:2107	addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC	2099:2218	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	12	37	theme	marked	2261:2266	arg1	overexpression					2268:2281	marked overexpression	2261:2281	marked overexpression	2261:2281	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	10	38	theme	core-fucosylated	1674:1689	arg1	m/z					1720:1722	m/z 1591, 1794	1720:1733	m/z	1720:1722	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	10	38	theme	core-fucosylated	1674:1689	arg1	glycans					1711:1717	two core-fucosylated and agalactosylated glycans	1670:1717	two core-fucosylated and agalactosylated glycans (m/z 1591, 1794)	1670:1734	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	5	39	theme	patients	771:778	arg1	Sera					760:763	Sera	760:763	Sera of BC patients belonging to stages I-IV and normal controls (NC)	760:828	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	9	40	theme	cancer	1581:1586	arg1	potential					1568:1576	greater invasive and metastatic potential	1536:1576	greater invasive and metastatic potential of cancer	1536:1586	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	10	41	from	NC	1825:1826	arg1	patients					1811:1818	(AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients	1767:1818	(AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC	1767:1826	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	2	42	theme	early	440:444	arg1	diagnosis					446:454	its early diagnosis	436:454	its early diagnosis	436:454	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	12	43	theme	distinguishing	2287:2300	arg1	ability					2302:2308	distinguishing ability	2287:2308	distinguishing ability	2287:2308	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	9	44	dep	invasive	1544:1551	arg1	greater					1536:1542	greater	1536:1542	greater	1536:1542	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	13	45	theme	deeper	2451:2456	arg1	understanding					2458:2470	a deeper understanding	2449:2470	a deeper understanding of specific biomarkers	2449:2493	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	13	46	theme	glycoprteomics	2412:2425	arg1	approach					2427:2434	a glycoprteomics approach	2410:2434	a glycoprteomics approach	2410:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	2	47	theme	serum	416:420	arg1	biomarker					422:430	no clinically validated serum biomarker	392:430	no clinically validated serum biomarker for its early diagnosis	392:454	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	7	48	theme	spectral	1019:1026	arg1	data					1028:1031	Mass spectral data	1014:1031	Mass spectral data	1014:1031	Mass spectral data were further processed and evaluated rigorously, using various bioinformatics and statistical tools.					
31208374	12	49	theme	black	2190:2194	arg1	women					2196:2200	native black women	2183:2200	native black women suffering from BC	2183:2218	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	10	50	theme	agalactosylated	1695:1709	arg1	m/z					1720:1722	m/z 1591, 1794	1720:1733	m/z	1720:1722	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	10	50	theme	agalactosylated	1695:1709	arg1	glycans					1711:1717	two core-fucosylated and agalactosylated glycans	1670:1717	two core-fucosylated and agalactosylated glycans (m/z 1591, 1794)	1670:1734	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	0	51	theme	G	35:35	arg1	N-glycome					37:45	immunoglobulin G N-glycome	20:45	immunoglobulin G N-glycome	20:45	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	51	theme	G	35:35	arg1	biomarkers					61:70	diagnostic biomarkers	50:70	diagnostic biomarkers for early detection of breast cancer in Ethiopian women	50:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	51	theme	G	35:35	arg1	serum					10:14	serum	10:14	serum	10:14	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	5	52	theme	normal	809:814	arg1	controls					816:823	normal controls	809:823	normal controls (NC)	809:828	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	5	52	theme	normal	809:814	arg1	NC					826:827	NC	826:827	NC	826:827	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	3	53	theme	whole	475:479	arg1	serum					481:485	whole serum	475:485	whole serum	475:485	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	12	54	theme	N-glycosylation	2153:2167	arg1	signatures					2169:2178	IgG N-glycosylation signatures	2149:2178	IgG N-glycosylation signatures	2149:2178	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	11	55	theme	structural	1847:1856	arg1	features					1858:1865	such structural features	1842:1865	such structural features	1842:1865	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	13	56	theme	glycoproteins	2390:2402	arg1	identification					2361:2374	direct identification	2354:2374	direct identification of the intact glycoproteins using a glycoprteomics approach	2354:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	3	57	theme	Immunoglobulin	500:513	arg1	IgG					518:520	IgG	518:520	IgG	518:520	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	3	57	theme	Immunoglobulin	500:513	arg1	G					515:515	Immunoglobulin G	500:515	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	1	58	theme	wide	246:249	arg1	range					251:255	a wide range	244:255	a wide range of diseases including cancer	244:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	1	58	theme	wide	246:249	arg1	cancer					279:284	cancer	279:284	cancer	279:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	6	59	theme	spin	944:947	arg1	plate					949:953	protein G spin plate	934:953	protein G spin plate	934:953	IgG was purified by affinity chromatography using protein G spin plate and further subjected to glycoblotting for glycan release.					
31208374	6	60	theme	protein	934:940	arg1	plate					949:953	protein G spin plate	934:953	protein G spin plate	934:953	IgG was purified by affinity chromatography using protein G spin plate and further subjected to glycoblotting for glycan release.					
31208374	11	61	from	Abundance	1829:1837	arg1	IgG					1870:1872	IgG	1870:1872	IgG	1870:1872	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	9	62	theme	invasive	1544:1551	arg1	potential					1568:1576	greater invasive and metastatic potential	1536:1576	greater invasive and metastatic potential of cancer	1536:1586	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	11	63	theme	aggressive	1997:2006	arg1	nature					2008:2013	the aggressive nature	1993:2013	the aggressive nature of BC commonly noticed in black population	1993:2056	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	13	64	theme	direct	2354:2359	arg1	identification					2361:2374	direct identification	2354:2374	direct identification of the intact glycoproteins using a glycoprteomics approach	2354:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	8	65	theme	complex	1253:1259	arg1	N-glycans					1266:1274	the NC, 17 complex type N-glycans	1242:1274	N-glycans	1266:1274	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	7	66	theme	statistical	1115:1125	arg1	tools					1127:1131	statistical tools	1115:1131	statistical tools	1115:1131	Mass spectral data were further processed and evaluated rigorously, using various bioinformatics and statistical tools.					
31208374	10	67	theme	stage	1802:1806	arg1	patients					1811:1818	(AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients	1767:1818	(AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC	1767:1826	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	8	68	theme	BC	1218:1219	arg1	patients					1221:1228	all BC patients	1214:1228	all BC patients compared to the NC, 17 complex type N-glycans	1214:1274	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	6	69	theme	glycan	998:1003	arg1	release					1005:1011	glycan release	998:1011	glycan release	998:1011	IgG was purified by affinity chromatography using protein G spin plate and further subjected to glycoblotting for glycan release.					
31208374	0	70	from	detection	82:90	arg1	women					122:126	Ethiopian women	112:126	Ethiopian women	112:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	8	71	theme	profound	1283:1290	arg1	abundance					1303:1311	profound expression abundance	1283:1311	profound expression abundance	1283:1311	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	3	72	theme	markers	588:594	arg1	identification					550:563	identification	550:563	identification of non-invasive glycan markers of BC	550:600	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	1	73	theme	biomarker	221:229	arg1	discovery					231:239	biomarker discovery	221:239	biomarker discovery in a wide range of diseases including cancer	221:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	9	74	gly	sialylated	1463:1472	arg1	structures					1474:1483	sialylated structures	1463:1483	sialylated structures	1463:1483	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	4	75	theme	comprehensive	625:637	arg1	approach					649:656	a comprehensive glycomics approach	623:656	a comprehensive glycomics approach	623:656	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	10	76	theme	BC	1648:1649	arg1	patients					1651:1658	BC patients	1648:1658	BC patients	1648:1658	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	11	77	theme	such	1842:1845	arg1	features					1858:1865	such structural features	1842:1865	such structural features	1842:1865	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	13	78	theme	clinical	2509:2516	arg1	utility					2518:2524	their clinical utility	2503:2524	their clinical utility	2503:2524	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	3	79	theme	non-invasive	568:579	arg1	markers					588:594	non-invasive glycan markers	568:594	non-invasive glycan markers of BC	568:600	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	1	80	from	discovery	231:239	arg1	range					251:255	a wide range	244:255	a wide range of diseases including cancer	244:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	1	80	from	discovery	231:239	arg1	cancer					279:284	cancer	279:284	cancer	279:284	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	9	81	dep	core-fucosylated	1423:1438	arg1	branched					1450:1457	branched	1450:1457	branched	1450:1457	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	9	81	dep	core-fucosylated	1423:1438	arg1	structures					1474:1483	sialylated structures	1463:1483	sialylated structures	1463:1483	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	12	82	theme	comprehensive	2075:2087	arg1	study					2089:2093	Our comprehensive study	2071:2093	Our comprehensive study	2071:2093	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	8	83	theme	diagnostic	1317:1326	arg1	AUC = 0.8-1					1339:1349	AUC = 0.8-1	1339:1349	AUC = 0.8-1	1339:1349	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	8	83	theme	diagnostic	1317:1326	arg1	potential					1328:1336	diagnostic potential	1317:1336	diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients	1317:1393	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	10	84	gly	core-fucosylated	1674:1689	arg1	m/z					1720:1722	m/z 1591, 1794	1720:1733	m/z	1720:1722	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	10	84	gly	core-fucosylated	1674:1689	arg1	glycans					1711:1717	two core-fucosylated and agalactosylated glycans	1670:1717	two core-fucosylated and agalactosylated glycans (m/z 1591, 1794)	1670:1734	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	13	85	theme	biomarkers	2484:2493	arg1	understanding					2458:2470	a deeper understanding	2449:2470	a deeper understanding of specific biomarkers	2449:2493	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	13	86	theme	specific	2475:2482	arg1	biomarkers					2484:2493	specific biomarkers	2475:2493	specific biomarkers	2475:2493	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	11	87	from	decrease	1895:1902	arg1	potential					1929:1937	its immunosuppressive potential	1907:1937	its immunosuppressive potential towards tumor cells	1907:1957	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	12	88	with	glyco-biomarkers	2239:2254	arg1	overexpression					2268:2281	marked overexpression	2261:2281	marked overexpression	2261:2281	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	12	88	with	glyco-biomarkers	2239:2254	arg1	ability					2302:2308	distinguishing ability	2287:2308	distinguishing ability	2287:2308	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	3	89	theme	BC	599:600	arg1	markers					588:594	non-invasive glycan markers	568:594	non-invasive glycan markers of BC	568:600	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
31208374	10	90	dep	patients	1811:1818	arg1	0.921					1784:1788	0.921	1784:1788	0.921	1784:1788	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	10	90	dep	patients	1811:1818	arg1	AUC = 0.944					1768:1778	AUC = 0.944	1768:1778	AUC = 0.944	1768:1778	N-glycans quantified form IgG confirmed their abundance in BC patients, of which two core-fucosylated and agalactosylated glycans (m/z 1591, 1794) could specifically distinguish (AUC = 0.944 and 0.921, p ≤ 0.001) stage II patients from NC.					
31208374	12	91	theme	early	2313:2317	arg1	patients					2325:2332	early stage patients	2313:2332	early stage patients	2313:2332	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	4	92	theme	glycan	693:698	arg1	purification					700:711	glycoblotting-based glycan purification	673:711	glycoblotting-based glycan purification	673:711	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	12	93	theme	novel	2233:2237	arg1	glyco-biomarkers					2239:2254	novel glyco-biomarkers	2233:2254	novel glyco-biomarkers with marked overexpression and distinguishing ability	2233:2308	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	1	94	theme	protein	155:161	arg1	patterns					177:184	protein glycosylation patterns	155:184	protein glycosylation patterns	155:184	BACKGROUND Alterations in protein glycosylation patterns have potentially been targeted for biomarker discovery in a wide range of diseases including cancer.					
31208374	0	95	theme	breast	95:100	arg1	cancer					102:107	breast cancer	95:107	breast cancer	95:107	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	9	96	theme	metastatic	1557:1566	arg1	potential					1568:1576	greater invasive and metastatic potential	1536:1576	greater invasive and metastatic potential of cancer	1536:1586	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	4	97	dep	METHODS	603:609	arg1	employed					614:621	employed	614:621	employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis	614:757	METHODS We employed a comprehensive glycomics approach by integrating glycoblotting-based glycan purification with MALDI-TOF/MS based quantitative analysis.					
31208374	8	98	theme	stage	1366:1370	arg1	patients					1386:1393	the early stage (I and II) BC patients	1356:1393	the early stage (I and II) BC patients	1356:1393	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	5	99	theme	BC	768:769	arg1	patients					771:778	BC patients	768:778	BC patients belonging to stages I-IV and normal controls (NC)	768:828	Sera of BC patients belonging to stages I-IV and normal controls (NC) were collected from Ethiopian women during 2015-2016.					
31208374	13	100	theme	Further	2335:2341	arg1	studies					2343:2349	Further studies	2335:2349	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach	2335:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	11	101	theme	immunosuppressive	1911:1927	arg1	potential					1929:1937	its immunosuppressive potential	1907:1937	its immunosuppressive potential towards tumor cells	1907:1957	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	0	102	theme	immunoglobulin	20:33	arg1	N-glycome					37:45	immunoglobulin G N-glycome	20:45	immunoglobulin G N-glycome	20:45	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	102	theme	immunoglobulin	20:33	arg1	biomarkers					61:70	diagnostic biomarkers	50:70	diagnostic biomarkers for early detection of breast cancer in Ethiopian women	50:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	102	theme	immunoglobulin	20:33	arg1	serum					10:14	serum	10:14	serum	10:14	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	8	103	theme	BC	1383:1384	arg1	patients					1386:1393	the early stage (I and II) BC patients	1356:1393	the early stage (I and II) BC patients	1356:1393	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	2	104	theme	validated	406:414	arg1	biomarker					422:430	no clinically validated serum biomarker	392:430	no clinically validated serum biomarker for its early diagnosis	392:454	Although there have been improvements in patient diagnosis and survival for breast cancer (BC), there is no clinically validated serum biomarker for its early diagnosis.					
31208374	12	105	dep	time	2123:2126	arg1	both					2128:2131	both	2128:2131	both	2128:2131	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	12	106	theme	native	2183:2188	arg1	women					2196:2200	native black women	2183:2200	native black women suffering from BC	2183:2218	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	13	107	from	studies	2343:2349	arg1	identification					2361:2374	direct identification	2354:2374	direct identification of the intact glycoproteins using a glycoprteomics approach	2354:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	0	108	theme	cancer	102:107	arg1	detection					82:90	early detection	76:90	early detection of breast cancer in Ethiopian women	76:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	109	theme	diagnostic	50:59	arg1	N-glycome					37:45	immunoglobulin G N-glycome	20:45	immunoglobulin G N-glycome	20:45	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	109	theme	diagnostic	50:59	arg1	biomarkers					61:70	diagnostic biomarkers	50:70	diagnostic biomarkers for early detection of breast cancer in Ethiopian women	50:126	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	0	109	theme	diagnostic	50:59	arg1	serum					10:14	serum	10:14	serum	10:14	Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.					
31208374	12	110	theme	women	2196:2200	arg1	signatures					2169:2178	IgG N-glycosylation signatures	2149:2178	IgG N-glycosylation signatures	2149:2178	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	12	110	theme	women	2196:2200	arg1	serum					2139:2143	whole serum	2133:2143	whole serum	2133:2143	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	13	111	theme	intact	2383:2388	arg1	glycoproteins					2390:2402	the intact glycoproteins	2379:2402	the intact glycoproteins using a glycoprteomics approach	2379:2434	Further studies on direct identification of the intact glycoproteins using a glycoprteomics approach will provide a deeper understanding of specific biomarkers towards their clinical utility.					
31208374	12	112	theme	IgG	2149:2151	arg1	signatures					2169:2178	IgG N-glycosylation signatures	2149:2178	IgG N-glycosylation signatures	2149:2178	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	11	113	theme	features	1858:1865	arg1	Abundance					1829:1837	Abundance	1829:1837	Abundance of such structural features in IgG	1829:1872	Abundance of such structural features in IgG is associated with a decrease in its immunosuppressive potential towards tumor cells, which in part may correlate with the aggressive nature of BC commonly noticed in black population.					
31208374	8	114	theme	early	1360:1364	arg1	stage					1366:1370	the early stage	1356:1370	the early stage (I and II) BC patients	1356:1393	RESULTS Out of 35 N-glycans that were significantly up-regulated in the sera of all BC patients compared to the NC, 17 complex type N-glycans showed profound expression abundance and diagnostic potential (AUC = 0.8-1) for the early stage (I and II) BC patients.					
31208374	12	115	theme	first	2117:2121	arg1	time					2123:2126	the first time	2113:2126	the first time	2113:2126	CONCLUSIONS Our comprehensive study has addressed for the first time both whole serum and IgG N-glycosylation signatures of native black women suffering from BC and revealed novel glyco-biomarkers with marked overexpression and distinguishing ability at early stage patients.					
31208374	9	116	gly	core-fucosylated	1423:1438	arg1	Most					1396:1399	Most	1396:1399	Most	1396:1399	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	9	116	gly	core-fucosylated	1423:1438	arg1	glycans					1410:1416	these glycans	1404:1416	these glycans	1404:1416	Most of these glycans were core-fucosylated, multiply branched and sialylated structures, whose abundance has been strongly associated with greater invasive and metastatic potential of cancer.					
31208374	3	117	theme	G	515:515	arg1	N-glycome					532:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	purified Immunoglobulin G (IgG) fraction N-glycome	491:540	Here, we profiled whole serum and purified Immunoglobulin G (IgG) fraction N-glycome towards identification of non-invasive glycan markers of BC.					
30983323	5	0	theme	smaller	797:803	arg1	mucins					817:822	smaller circulatory mucins	797:822	smaller circulatory mucins protruding into the blood circulation	797:860	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	3	1	theme	detected	524:531	arg1	components					533:542	far more detected components	515:542	far more detected components than shown in any previous studies	515:577	This is far more detected components than shown in any previous studies.					
30983323	2	2	from	cleavage	325:332	arg1	procedure					367:375	this procedure	362:375	this procedure	362:375	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	6	3	contain	have	1138:1141	arg1	we					1128:1129	we	1128:1129	we	1128:1129	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	6	3	contain	have	1138:1141	arg2	explanation					1151:1161	a ready explanation	1143:1161	a ready explanation due to lack of any literature describing hormonal control of O-glycosylation	1143:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	5	4	theme	circulatory	805:815	arg1	mucins					817:822	smaller circulatory mucins	797:822	smaller circulatory mucins protruding into the blood circulation	797:860	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	1	5	theme	serum	177:181	arg1	O-glycans					183:191	serum O-glycans	177:191	serum O-glycans	177:191	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	2	6	from	steps	353:357	arg1	procedure					367:375	this procedure	362:375	this procedure	362:375	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	5	7	theme	serum	916:920	arg1	samples					922:928	the serum samples	912:928	the serum samples	912:928	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	0	8	theme	Cancer	108:113	arg1	Patients					115:122	Colorectal Cancer Patients	97:122	Colorectal Cancer Patients	97:122	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	2	9	theme	simultaneous	312:323	arg1	cleavage					325:332	The simultaneous cleavage and permethylation steps	308:357	cleavage	325:332	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	1	10	theme	colorectal	280:289	arg1	patients					298:305	colorectal cancer patients	280:305	colorectal cancer patients	280:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	0	11	theme	Colorectal	97:106	arg1	Patients					115:122	Colorectal Cancer Patients	97:122	Colorectal Cancer Patients	97:122	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	6	12	theme	literature	1182:1191	arg1	lack					1170:1173	lack	1170:1173	lack of any literature describing hormonal control of O-glycosylation	1170:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	1	13	theme	cancer	291:296	arg1	patients					298:305	colorectal cancer patients	280:305	colorectal cancer patients	280:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	7	14	theme	analytical	1299:1308	arg1	technologies					1310:1321	new analytical technologies	1295:1321	new analytical technologies	1295:1321	Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.					
30983323	4	15	theme	tests	654:658	arg1	battery					631:637	a battery	629:637	a battery of statistical tests	629:658	The analytical results were further subjected to a battery of statistical tests.					
30983323	6	16	theme	glycosylation	1017:1029	arg1	trends					1031:1036	the expected glycosylation trends	1004:1036	the expected glycosylation trends	1004:1036	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	7	17	theme	sample	1349:1354	arg1	sets					1356:1359	clinically interesting sample sets	1326:1359	clinically interesting sample sets	1326:1359	Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.					
30983323	6	18	theme	surprising	1099:1108	arg1	results					1110:1116	some surprising results	1094:1116	some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation	1094:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	0	19	theme	Sensitive	7:15	arg1	Profiling					26:34	Highly Sensitive O-Glycan Profiling	0:34	Highly Sensitive O-Glycan Profiling for Human Serum Proteins	0:59	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	6	20	theme	expected	1008:1015	arg1	trends					1031:1036	the expected glycosylation trends	1004:1036	the expected glycosylation trends	1004:1036	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	7	21	theme	new	1295:1297	arg1	technologies					1310:1321	new analytical technologies	1295:1321	new analytical technologies	1295:1321	Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.					
30983323	2	22	theme	O-glycans	418:426	arg1	integrity					390:398	the integrity	386:398	the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile	386:504	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	1	23	theme	patients	298:305	arg1	cohorts					269:275	different cohorts	259:275	different cohorts of colorectal cancer patients	259:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	5	24	theme	O-glycan	665:672	arg1	compositions					674:685	Our O-glycan compositions	661:685	Our O-glycan compositions	661:685	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	2	25	theme	permethylation	338:351	arg1	steps					353:357	The simultaneous cleavage and permethylation steps	308:357	steps	353:357	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	2	26	theme	minor	412:416	arg1	O-glycans					418:426	released minor O-glycans	403:426	released minor O-glycans	403:426	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	6	27	theme	subjects	1073:1080	arg1	comparisons					1043:1053	the comparisons	1039:1053	the comparisons of male vs female subjects	1039:1080	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	7	28	theme	interesting	1337:1347	arg1	sets					1356:1359	clinically interesting sample sets	1326:1359	clinically interesting sample sets	1326:1359	Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.					
30983323	0	29	theme	O-Glycan	17:24	arg1	Profiling					26:34	Highly Sensitive O-Glycan Profiling	0:34	Highly Sensitive O-Glycan Profiling for Human Serum Proteins	0:59	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	5	30	theme	solid	745:749	arg1	tumors					751:756	solid tumors	745:756	solid tumors	745:756	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	2	31	theme	O-linked	440:447	arg1	oligosaccharides					449:464	39 O-linked oligosaccharides	437:464	39 O-linked oligosaccharides	437:464	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	0	32	theme	Human	40:44	arg1	Proteins					52:59	Human Serum Proteins	40:59	Human Serum Proteins	40:59	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	4	33	theme	analytical	584:593	arg1	results					595:601	The analytical results	580:601	The analytical results	580:601	The analytical results were further subjected to a battery of statistical tests.					
30983323	0	34	from	Changes	86:92	arg1	Patients					115:122	Colorectal Cancer Patients	97:122	Colorectal Cancer Patients	97:122	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	1	35	theme	multiple	222:229	arg1	samples					237:243	multiple serum samples	222:243	multiple serum samples obtained from different cohorts of colorectal cancer patients	222:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	5	36	theme	cell	769:772	arg1	lines					774:778	cancer cell lines	762:778	cancer cell lines	762:778	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	5	37	theme	O-glycans	883:891	arg1	source					873:878	one source	869:878	one source of O-glycans that we observe in the serum samples	869:928	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	1	38	theme	serum	231:235	arg1	samples					237:243	multiple serum samples	222:243	multiple serum samples obtained from different cohorts of colorectal cancer patients	222:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	5	39	theme	cancer	762:767	arg1	lines					774:778	cancer cell lines	762:778	cancer cell lines	762:778	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	6	40	theme	female	1066:1071	arg1	subjects					1073:1080	male vs female subjects	1058:1080	male vs female subjects	1058:1080	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	6	41	theme	ready	1145:1149	arg1	explanation					1151:1161	a ready explanation	1143:1161	a ready explanation due to lack of any literature describing hormonal control of O-glycosylation	1143:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	0	42	theme	Serum	46:50	arg1	Proteins					52:59	Human Serum Proteins	40:59	Human Serum Proteins	40:59	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	6	43	theme	statistical	959:969	arg1	comparisons					971:981	the control vs cancer statistical comparisons	937:981	comparisons	971:981	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	5	44	theme	previous	714:721	arg1	results					723:729	the previous results	710:729	the previous results obtained with solid tumors and cancer cell lines	710:778	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	3	45	dep	components	533:542	arg1	shown					549:553	shown	549:553	shown in any previous studies	549:577	This is far more detected components than shown in any previous studies.					
30983323	6	46	theme	cancer	952:957	arg1	comparisons					971:981	the control vs cancer statistical comparisons	937:981	comparisons	971:981	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	6	47	theme	O-glycosylation	1224:1238	arg1	control					1213:1219	hormonal control	1204:1219	hormonal control of O-glycosylation	1204:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	1	48	theme	developed	133:141	arg1	protocol					154:161	A newly developed microscale protocol	125:161	A newly developed microscale protocol for profiling serum O-glycans	125:191	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	6	49	theme	due	1163:1165	arg1	explanation					1151:1161	a ready explanation	1143:1161	a ready explanation due to lack of any literature describing hormonal control of O-glycosylation	1143:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	3	50	theme	previous	562:569	arg1	studies					571:577	any previous studies	558:577	any previous studies	558:577	This is far more detected components than shown in any previous studies.					
30983323	1	51	theme	microscale	143:152	arg1	protocol					154:161	A newly developed microscale protocol	125:161	A newly developed microscale protocol for profiling serum O-glycans	125:191	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30983323	6	52	theme	male	1058:1061	arg1	subjects					1073:1080	male vs female subjects	1058:1080	male vs female subjects	1058:1080	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	0	53	theme	Gender-Dependent	69:84	arg1	Changes					86:92	Gender-Dependent Changes	69:92	Gender-Dependent Changes in Colorectal Cancer Patients	69:122	Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.					
30983323	2	54	link	O-linked	440:447	arg1	oligosaccharides					449:464	39 O-linked oligosaccharides	437:464	39 O-linked oligosaccharides	437:464	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	2	55	theme	released	403:410	arg1	O-glycans					418:426	released minor O-glycans	403:426	released minor O-glycans	403:426	The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile.					
30983323	4	56	theme	statistical	642:652	arg1	tests					654:658	statistical tests	642:658	statistical tests	642:658	The analytical results were further subjected to a battery of statistical tests.					
30983323	6	57	theme	hormonal	1204:1211	arg1	control					1213:1219	hormonal control	1204:1219	hormonal control of O-glycosylation	1204:1238	While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation.					
30983323	5	58	theme	blood	844:848	arg1	circulation					850:860	the blood circulation	840:860	the blood circulation	840:860	Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples.					
30983323	1	59	theme	different	259:267	arg1	cohorts					269:275	different cohorts	259:275	different cohorts of colorectal cancer patients	259:305	A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients.					
30030954	4	0	theme	mass	789:792	arg1	spectrometry					794:805	mass spectrometry	789:805	mass spectrometry	789:805	The released glycan structures were analyzed and confirmed with mass spectrometry.					
30030954	6	1	theme	lactoferrin	1143:1153	arg1	function					1131:1138	the antimicrobial function	1113:1138	the antimicrobial function of lactoferrin	1113:1153	These results suggest that sialylated glycans play important role in the antimicrobial function of lactoferrin.					
30030954	3	2	theme	glycan	576:581	arg1	profile					583:589	different glycan profile	566:589	different glycan profile	566:589	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	6	3	theme	important	1095:1103	arg1	role					1105:1108	important role	1095:1108	important role	1095:1108	These results suggest that sialylated glycans play important role in the antimicrobial function of lactoferrin.					
30030954	0	4	theme	antimicrobial	80:92	arg1	activity					94:101	antimicrobial activity	80:101	antimicrobial activity of lactoferrin	80:116	Selective deglycosylation of lactoferrin to understand glycans' contribution to antimicrobial activity of lactoferrin.					
30030954	5	5	theme	MIC	985:987	arg1	6mg/ml					1036:1041	6mg/ml	1036:1041	6mg/ml	1036:1041	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	5	theme	MIC	985:987	arg1	value					989:993	the MIC value	981:993	the MIC value for fully deglycosylated lactoferrin	981:1030	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	0	6	theme	lactoferrin	106:116	arg1	activity					94:101	antimicrobial activity	80:101	antimicrobial activity of lactoferrin	80:116	Selective deglycosylation of lactoferrin to understand glycans' contribution to antimicrobial activity of lactoferrin.					
30030954	3	7	theme	native	652:657	arg1	glycan					696:701	native, fully deglycosylated and sialylated glycan	652:701	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	1	8	contain	contains	183:190	arg1	protein					170:176	a highly glycosylated antimicrobial protein	134:176	a highly glycosylated antimicrobial protein that contains multiple glycan types	134:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	8	contain	contains	183:190	arg1	Lactoferrin					119:129	Lactoferrin	119:129	Lactoferrin	119:129	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	8	contain	contains	183:190	arg2	types					208:212	multiple glycan types	192:212	multiple glycan types	192:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	5	9	gly	sialylated	843:852	arg1	glycans					854:860	native and sialylated glycans	832:860	native and sialylated glycans enriched lactoferrin	832:881	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	3	10	dep	enzymes	643:649	arg1	glycan					696:701	native, fully deglycosylated and sialylated glycan	652:701	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	5	11	theme	E.coli	950:955	arg1	DH5a					957:960	E.coli DH5a	950:960	E.coli DH5a (1 mg/ml)	950:970	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	11	theme	E.coli	950:955	arg1	mg/ml					965:969	1 mg/ml	963:969	1 mg/ml	963:969	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	12	theme	native	832:837	arg1	glycans					854:860	native and sialylated glycans	832:860	native and sialylated glycans enriched lactoferrin	832:881	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	2	13	theme	glycan	478:483	arg1	types					485:489	specific glycan types	469:489	specific glycan types	469:489	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	1	14	theme	multiple	192:199	arg1	types					208:212	multiple glycan types	192:212	multiple glycan types	192:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	6	15	theme	sialylated	1071:1080	arg1	glycans					1082:1088	sialylated glycans	1071:1088	sialylated glycans	1071:1088	These results suggest that sialylated glycans play important role in the antimicrobial function of lactoferrin.					
30030954	3	16	with	forms	555:559	arg1	profile					583:589	different glycan profile	566:589	different glycan profile	566:589	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	2	17	theme	antimicrobial	498:510	arg1	function					512:519	the antimicrobial function	494:519	the antimicrobial function of lactoferrin	494:534	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	1	18	theme	glycan	201:206	arg1	types					208:212	multiple glycan types	192:212	multiple glycan types	192:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	5	19	theme	concentration	915:927	arg1	values					935:940	similar minimum inhibitory concentration (MIC) values	888:940	similar minimum inhibitory concentration (MIC) values	888:940	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	2	20	used	used	385:388	arg2	forms					262:266	three forms	256:266	recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide)	233:378	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	3	21	gly	sialylated	685:694	arg1	glycan					696:701	native, fully deglycosylated and sialylated glycan	652:701	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	0	22	theme	Selective	0:8	arg1	deglycosylation					10:24	Selective deglycosylation	0:24	Selective deglycosylation of lactoferrin	0:39	Selective deglycosylation of lactoferrin to understand glycans' contribution to antimicrobial activity of lactoferrin.					
30030954	2	23	dep	endo-β-N-acetylglucosaminidase	277:306	arg1	polyhistide					367:377	polyhistide	367:377	polyhistide	367:377	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	2	23	dep	endo-β-N-acetylglucosaminidase	277:306	arg1	Glutatiohine-S-transferase					336:361	free, genetically attached Glutatiohine-S-transferase	309:361	free, genetically attached Glutatiohine-S-transferase	309:361	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	0	24	theme	lactoferrin	29:39	arg1	deglycosylation					10:24	Selective deglycosylation	0:24	Selective deglycosylation of lactoferrin	0:39	Selective deglycosylation of lactoferrin to understand glycans' contribution to antimicrobial activity of lactoferrin.					
30030954	3	25	dep	native	652:657	arg1	deglycosylated					666:679	deglycosylated	666:679	deglycosylated	666:679	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	3	26	theme	fusion	629:634	arg1	enzymes					643:649	these fusion tagged enzymes	623:649	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	2	27	theme	types	485:489	arg1	contribution					453:464	the contribution	449:464	the contribution of specific glycan types to the antimicrobial function of lactoferrin	449:534	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	3	28	theme	tagged	636:641	arg1	enzymes					643:649	these fusion tagged enzymes	623:649	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	4	29	theme	glycan	738:743	arg1	structures					745:754	The released glycan structures	725:754	The released glycan structures	725:754	The released glycan structures were analyzed and confirmed with mass spectrometry.					
30030954	1	30	theme	antimicrobial	156:168	arg1	protein					170:176	a highly glycosylated antimicrobial protein	134:176	a highly glycosylated antimicrobial protein that contains multiple glycan types	134:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	30	theme	antimicrobial	156:168	arg1	Lactoferrin					119:129	Lactoferrin	119:129	Lactoferrin	119:129	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	31	gly	glycosylated	143:154	arg1	protein					170:176	a highly glycosylated antimicrobial protein	134:176	a highly glycosylated antimicrobial protein that contains multiple glycan types	134:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	31	gly	glycosylated	143:154	arg1	Lactoferrin					119:129	Lactoferrin	119:129	Lactoferrin	119:129	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	4	32	theme	released	729:736	arg1	structures					745:754	The released glycan structures	725:754	The released glycan structures	725:754	The released glycan structures were analyzed and confirmed with mass spectrometry.					
30030954	2	33	theme	specific	469:476	arg1	types					485:489	specific glycan types	469:489	specific glycan types	469:489	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	3	34	with	treatment	608:616	arg1	enzymes					643:649	these fusion tagged enzymes	623:649	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	5	35	theme	similar	888:894	arg1	values					935:940	similar minimum inhibitory concentration (MIC) values	888:940	similar minimum inhibitory concentration (MIC) values	888:940	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	0	36	gly	deglycosylation	10:24	arg1	lactoferrin					29:39	lactoferrin	29:39	lactoferrin	29:39	Selective deglycosylation of lactoferrin to understand glycans' contribution to antimicrobial activity of lactoferrin.					
30030954	2	37	theme	lactoferrin	524:534	arg1	function					512:519	the antimicrobial function	494:519	the antimicrobial function of lactoferrin	494:534	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	5	38	gly	deglycosylated	1005:1018	arg1	lactoferrin					1020:1030	fully deglycosylated lactoferrin	999:1030	fully deglycosylated lactoferrin	999:1030	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	2	39	theme	produced	247:254	arg1	forms					262:266	three forms	256:266	recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide)	233:378	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	2	40	theme	selective	394:402	arg1	release					404:410	selective release	394:410	selective release of lactoferrin glycans	394:433	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	5	41	theme	inhibitory	904:913	arg1	MIC					930:932	MIC	930:932	MIC	930:932	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	41	theme	inhibitory	904:913	arg1	concentration					915:927	minimum inhibitory concentration	896:927	similar minimum inhibitory concentration (MIC) values	888:940	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	6	42	gly	sialylated	1071:1080	arg1	glycans					1082:1088	sialylated glycans	1071:1088	sialylated glycans	1071:1088	These results suggest that sialylated glycans play important role in the antimicrobial function of lactoferrin.					
30030954	3	43	theme	different	566:574	arg1	profile					583:589	different glycan profile	566:589	different glycan profile	566:589	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	3	44	theme	lactoferrin	543:553	arg1	forms					555:559	Three lactoferrin forms	537:559	Three lactoferrin forms with different glycan profile	537:589	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	6	45	theme	antimicrobial	1117:1129	arg1	function					1131:1138	the antimicrobial function	1113:1138	the antimicrobial function of lactoferrin	1113:1153	These results suggest that sialylated glycans play important role in the antimicrobial function of lactoferrin.					
30030954	2	46	theme	free	309:312	arg1	Glutatiohine-S-transferase					336:361	free, genetically attached Glutatiohine-S-transferase	309:361	free, genetically attached Glutatiohine-S-transferase	309:361	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	2	47	theme	endo-β-N-acetylglucosaminidase	277:306	arg1	forms					262:266	three forms	256:266	recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide)	233:378	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	1	48	theme	glycosylated	143:154	arg1	protein					170:176	a highly glycosylated antimicrobial protein	134:176	a highly glycosylated antimicrobial protein that contains multiple glycan types	134:212	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	1	48	theme	glycosylated	143:154	arg1	Lactoferrin					119:129	Lactoferrin	119:129	Lactoferrin	119:129	Lactoferrin is a highly glycosylated antimicrobial protein that contains multiple glycan types.					
30030954	5	49	theme	deglycosylated	1005:1018	arg1	lactoferrin					1020:1030	fully deglycosylated lactoferrin	999:1030	fully deglycosylated lactoferrin	999:1030	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	50	theme	sialylated	843:852	arg1	glycans					854:860	native and sialylated glycans	832:860	native and sialylated glycans enriched lactoferrin	832:881	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	51	contain	have	883:886	arg1	glycans					854:860	native and sialylated glycans	832:860	native and sialylated glycans enriched lactoferrin	832:881	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	51	contain	have	883:886	arg2	values					935:940	similar minimum inhibitory concentration (MIC) values	888:940	similar minimum inhibitory concentration (MIC) values	888:940	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	2	52	theme	novel	271:275	arg1	endo-β-N-acetylglucosaminidase					277:306	novel endo-β-N-acetylglucosaminidase	271:306	novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide)	271:378	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	2	53	theme	glycans	427:433	arg1	release					404:410	selective release	394:410	selective release of lactoferrin glycans	394:433	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	2	54	dep	free	309:312	arg1	attached					327:334	attached	327:334	attached	327:334	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30030954	5	55	theme	minimum	896:902	arg1	MIC					930:932	MIC	930:932	MIC	930:932	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	5	55	theme	minimum	896:902	arg1	concentration					915:927	minimum inhibitory concentration	896:927	similar minimum inhibitory concentration (MIC) values	888:940	The results showed that native and sialylated glycans enriched lactoferrin have similar minimum inhibitory concentration (MIC) values against E.coli DH5a (1 mg/ml), whereas the MIC value for fully deglycosylated lactoferrin was 6mg/ml.					
30030954	3	56	theme	sialylated	685:694	arg1	glycan					696:701	native, fully deglycosylated and sialylated glycan	652:701	these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin	623:722	Three lactoferrin forms with different glycan profile were obtained by treatment with these fusion tagged enzymes; native, fully deglycosylated and sialylated glycan enriched lactoferrin.					
30030954	2	57	theme	lactoferrin	415:425	arg1	glycans					427:433	lactoferrin glycans	415:433	lactoferrin glycans	415:433	In this research, recombinantly produced three forms of novel endo-β-N-acetylglucosaminidase (free, genetically attached Glutatiohine-S-transferase and polyhistide) were used for selective release of lactoferrin glycans to understand the contribution of specific glycan types to the antimicrobial function of lactoferrin.					
30427586	7	0	theme	ions	1301:1304	arg1	abundance					1280:1288	the relative abundance	1267:1288	the relative abundance of oxonium ions to retrieve glycan structure information	1267:1345	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	8	1	from	comprehensiveness	1574:1590	arg1	glycoproteomics					1624:1638	manual and software-assisted glycoproteomics	1595:1638	manual and software-assisted glycoproteomics	1595:1638	The findings may increase confidence and comprehensiveness in manual and software-assisted glycoproteomics.					
30427586	1	2	theme	spectrometric	148:160	arg1	identification					162:175	unambiguous mass spectrometric identification	131:175	unambiguous mass spectrometric identification	131:175	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	0	3	theme	Glycoproteomic	49:62	arg1	Analyses-Exploiting					64:82	In-Depth Glycoproteomic Analyses-Exploiting	40:82	In-Depth Glycoproteomic Analyses-Exploiting	40:82	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	6	4	gly	N-glycopeptides	1201:1215	arg2	N-glycopeptides					1201:1215	all N-glycopeptides	1197:1215	all N-glycopeptides	1197:1215	It is shown for the first time that this fragmentation signature can consistently be found across all N-glycopeptides, but not on O-glycopeptides.					
30427586	2	5	theme	site-specific	379:391	arg1	analysis					393:400	the in-depth and site-specific analysis	362:400	the in-depth and site-specific analysis of N- and O-glycopeptides	362:426	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	1	6	theme	glycoproteins	276:288	arg1	macroheterogeneity					254:271	macroheterogeneity	254:271	macroheterogeneity	254:271	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	1	6	theme	glycoproteins	276:288	arg1	micro-					243:248	micro-	243:248	micro-	243:248	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	0	7	theme	Diagnostic	88:97	arg1	Potential					99:107	the Diagnostic Potential	84:107	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	4	8	gly	glycopeptide	897:908	arg2	glycopeptide					897:908	glycopeptide enrichment	897:919	glycopeptide enrichment	897:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	1	9	gly	glycopeptides	201:213	arg2	glycopeptides					201:213	glycopeptides	201:213	glycopeptides	201:213	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	7	10	theme	high-mannose-type	1392:1408	arg1	N-glycans					1410:1418	hybrid- and high-mannose-type N-glycans	1380:1418	hybrid- and high-mannose-type N-glycans	1380:1418	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	4	11	theme	interaction	830:840	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	5	12	theme	+H+0,2	1068:1073	arg1	X GlcNAc					1075:1082	employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1025:1082	rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1018:1082	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	7	13	theme	oxonium	1293:1299	arg1	ions					1301:1304	oxonium ions	1293:1304	oxonium ions	1293:1304	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	5	14	gly	N-glycopeptides	973:987	arg2	N-glycopeptides					973:987	N-glycopeptides	973:987	N-glycopeptides	973:987	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	1	15	gly	macroheterogeneity	254:271	arg1	glycoproteins					276:288	glycoproteins	276:288	glycoproteins	276:288	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	4	16	theme	hydrophilic	818:828	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	4	17	theme	modified	778:785	arg1	version					800:806	a modified and improved version	776:806	a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	776:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	7	18	theme	relative	1271:1278	arg1	abundance					1280:1288	the relative abundance	1267:1288	the relative abundance of oxonium ions to retrieve glycan structure information	1267:1345	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	5	19	theme	signature	1048:1056	arg1	X GlcNAc					1075:1082	employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1025:1082	rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1018:1082	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	7	20	theme	N-glycans	1410:1418	arg1	differentiation					1361:1375	differentiation	1361:1375	differentiation	1361:1375	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	4	21	theme	chromatography-based	849:868	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	5	22	theme	N-glycopeptides	973:987	arg1	identification					955:968	the unambiguous identification	939:968	the unambiguous identification of N-glycopeptides	939:987	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	3	23	theme	representative	461:474	arg1	fibrinogen					529:538	fibrinogen	529:538	fibrinogen	529:538	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	23	theme	representative	461:474	arg1	IgG					524:526	IgG	524:526	IgG	524:526	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	23	theme	representative	461:474	arg1	glycoproteins					509:521	four representative and biopharmaceutically relevant glycoproteins	456:521	four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	456:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	23	theme	representative	461:474	arg1	lactotransferrin					541:556	lactotransferrin	541:556	lactotransferrin	541:556	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	23	theme	representative	461:474	arg1	ribonuclease B					563:576	ribonuclease B	563:576	ribonuclease B	563:576	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	7	24	theme	abundance	1280:1288	arg1	use					1260:1262	the use	1256:1262	the use of the relative abundance of oxonium ions to retrieve glycan structure information	1256:1345	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	5	25	theme	a	1001:1001	arg1	use					994:996	the use	990:996	the use of a	990:1001	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	1	26	gly	glycoproteins	276:288	arg1	glycoproteins					276:288	glycoproteins	276:288	glycoproteins	276:288	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	4	27	theme	liquid	842:847	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	4	28	theme	solid	870:874	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	0	29	theme	Fine	4:7	arg1	Art					9:11	The Fine Art	0:11	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	5	30	theme	employed-fragmentation	1025:1046	arg1	X GlcNAc					1075:1082	employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1025:1082	rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1018:1082	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	2	31	theme	O-glycopeptides	412:426	arg1	analysis					393:400	the in-depth and site-specific analysis	362:400	the in-depth and site-specific analysis of N- and O-glycopeptides	362:426	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	2	32	gly	O-glycopeptides	412:426	arg2	O-glycopeptides					412:426	O-glycopeptides	412:426	O-glycopeptides	412:426	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	2	33	theme	energy	337:342	arg1	fragmentation					344:356	stepped collisional energy fragmentation	317:356	stepped collisional energy fragmentation	317:356	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	8	34	from	confidence	1559:1568	arg1	glycoproteomics					1624:1638	manual and software-assisted glycoproteomics	1595:1638	manual and software-assisted glycoproteomics	1595:1638	The findings may increase confidence and comprehensiveness in manual and software-assisted glycoproteomics.					
30427586	1	35	theme	glycopeptides	201:213	arg1	characterization					181:196	characterization	181:196	characterization	181:196	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	1	35	theme	glycopeptides	201:213	arg1	identification					162:175	unambiguous mass spectrometric identification	131:175	unambiguous mass spectrometric identification	131:175	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	6	36	gly	O-glycopeptides	1229:1243	arg2	O-glycopeptides					1229:1243	O-glycopeptides	1229:1243	O-glycopeptides	1229:1243	It is shown for the first time that this fragmentation signature can consistently be found across all N-glycopeptides, but not on O-glycopeptides.					
30427586	4	37	theme	improved	791:798	arg1	version					800:806	a modified and improved version	776:806	a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	776:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	0	38	theme	Ions	121:124	arg1	Potential					99:107	the Diagnostic Potential	84:107	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	2	39	theme	collisional	325:335	arg1	fragmentation					344:356	stepped collisional energy fragmentation	317:356	stepped collisional energy fragmentation	317:356	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	3	40	theme	glycoproteins	509:521	arg1	ribonuclease B					563:576	ribonuclease B	563:576	ribonuclease B	563:576	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	40	theme	glycoproteins	509:521	arg1	IgG					524:526	IgG	524:526	IgG	524:526	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	40	theme	glycoproteins	509:521	arg1	fibrinogen					529:538	fibrinogen	529:538	fibrinogen	529:538	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	40	theme	glycoproteins	509:521	arg1	set					449:451	a set	447:451	a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	447:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	40	theme	glycoproteins	509:521	arg1	glycoproteins					509:521	four representative and biopharmaceutically relevant glycoproteins	456:521	four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	456:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	40	theme	glycoproteins	509:521	arg1	lactotransferrin					541:556	lactotransferrin	541:556	lactotransferrin	541:556	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	1	41	dep	identification	162:175	arg1	The					127:129	The	127:129	The	127:129	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	0	42	theme	Fragment	112:119	arg1	Ions					121:124	Fragment Ions	112:124	Fragment Ions	112:124	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	3	43	theme	high-quality	699:710	arg1	N-					721:722	high-quality in-depth N-	699:722	high-quality in-depth N-	699:722	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	2	44	theme	stepped	317:323	arg1	fragmentation					344:356	stepped collisional energy fragmentation	317:356	stepped collisional energy fragmentation	317:356	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	1	45	theme	unambiguous	131:141	arg1	identification					162:175	unambiguous mass spectrometric identification	131:175	unambiguous mass spectrometric identification	131:175	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	3	46	theme	in-depth	712:719	arg1	N-					721:722	high-quality in-depth N-	699:722	high-quality in-depth N-	699:722	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	0	47	dep	Guide	31:35	arg1	Potential					99:107	the Diagnostic Potential	84:107	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	7	48	theme	differentiation	1423:1437	arg1	differentiation					1361:1375	differentiation	1361:1375	differentiation	1361:1375	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	7	49	theme	hybrid-	1380:1386	arg1	N-glycans					1410:1418	hybrid- and high-mannose-type N-glycans	1380:1418	hybrid- and high-mannose-type N-glycans	1380:1418	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	0	50	theme	Destruction	16:26	arg1	Art					9:11	The Fine Art	0:11	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	5	51	dep	conserved	1003:1011	arg1	X GlcNAc					1075:1082	employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1025:1082	rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1018:1082	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	8	52	theme	manual	1595:1600	arg1	glycoproteomics					1624:1638	manual and software-assisted glycoproteomics	1595:1638	manual and software-assisted glycoproteomics	1595:1638	The findings may increase confidence and comprehensiveness in manual and software-assisted glycoproteomics.					
30427586	3	53	gly	glycoproteins	509:521	arg1	fibrinogen					529:538	fibrinogen	529:538	fibrinogen	529:538	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	53	gly	glycoproteins	509:521	arg1	IgG					524:526	IgG	524:526	IgG	524:526	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	53	gly	glycoproteins	509:521	arg1	glycoproteins					509:521	four representative and biopharmaceutically relevant glycoproteins	456:521	four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	456:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	53	gly	glycoproteins	509:521	arg1	lactotransferrin					541:556	lactotransferrin	541:556	lactotransferrin	541:556	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	53	gly	glycoproteins	509:521	arg1	ribonuclease B					563:576	ribonuclease B	563:576	ribonuclease B	563:576	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	54	theme	relevant	500:507	arg1	fibrinogen					529:538	fibrinogen	529:538	fibrinogen	529:538	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	54	theme	relevant	500:507	arg1	IgG					524:526	IgG	524:526	IgG	524:526	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	54	theme	relevant	500:507	arg1	glycoproteins					509:521	four representative and biopharmaceutically relevant glycoproteins	456:521	four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	456:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	54	theme	relevant	500:507	arg1	lactotransferrin					541:556	lactotransferrin	541:556	lactotransferrin	541:556	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	54	theme	relevant	500:507	arg1	ribonuclease B					563:576	ribonuclease B	563:576	ribonuclease B	563:576	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	8	55	theme	software-assisted	1606:1622	arg1	glycoproteomics					1624:1638	manual and software-assisted glycoproteomics	1595:1638	manual and software-assisted glycoproteomics	1595:1638	The findings may increase confidence and comprehensiveness in manual and software-assisted glycoproteomics.					
30427586	1	56	dep	micro-	243:248	arg1	the					239:241	the	239:241	the	239:241	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	3	57	theme	developed	616:624	arg1	workflow					626:633	the developed workflow	612:633	the developed workflow	612:633	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	5	58	theme	[Mpeptide	1058:1066	arg1	X GlcNAc					1075:1082	employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1025:1082	rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc	1018:1082	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	3	59	theme	O-glycoproteomic	728:743	arg1	analyses					745:752	O-glycoproteomic analyses	728:752	O-glycoproteomic analyses	728:752	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	60	theme	workflow	626:633	arg1	limitations					597:607	limitations	597:607	limitations	597:607	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	60	theme	workflow	626:633	arg1	benefits					584:591	benefits	584:591	benefits	584:591	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	2	61	theme	in-depth	366:373	arg1	analysis					393:400	the in-depth and site-specific analysis	362:400	the in-depth and site-specific analysis of N- and O-glycopeptides	362:426	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	4	62	theme	phase	876:880	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	3	63	theme	state-of-the-art	657:672	arg1	blueprint					674:682	a state-of-the-art blueprint	655:682	a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses	655:752	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	4	64	theme	glycopeptide	897:908	arg1	enrichment					910:919	glycopeptide enrichment	897:919	glycopeptide enrichment	897:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	4	65	theme	cotton	811:816	arg1	extraction					882:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction	811:891	cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	811:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	6	66	theme	first	1119:1123	arg1	time					1125:1128	the first time	1115:1128	the first time	1115:1128	It is shown for the first time that this fragmentation signature can consistently be found across all N-glycopeptides, but not on O-glycopeptides.					
30427586	0	67	dep	Art	9:11	arg1	Guide					31:35	A Guide	29:35	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.	0:125	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	3	68	dep	benefits	584:591	arg1	the					580:582	the	580:582	the	580:582	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	0	69	theme	In-Depth	40:47	arg1	Analyses-Exploiting					64:82	In-Depth Glycoproteomic Analyses-Exploiting	40:82	In-Depth Glycoproteomic Analyses-Exploiting	40:82	The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses-Exploiting the Diagnostic Potential of Fragment Ions.					
30427586	5	70	theme	unambiguous	943:953	arg1	identification					955:968	the unambiguous identification	939:968	the unambiguous identification of N-glycopeptides	939:987	For the unambiguous identification of N-glycopeptides, the use of a conserved yet, rarely employed-fragmentation signature [Mpeptide +H+0,2 X GlcNAc]+ is proposed.					
30427586	3	71	dep	glycoproteins	509:521	arg1	fibrinogen					529:538	fibrinogen	529:538	fibrinogen	529:538	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	71	dep	glycoproteins	509:521	arg1	IgG					524:526	IgG	524:526	IgG	524:526	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	71	dep	glycoproteins	509:521	arg1	glycoproteins					509:521	four representative and biopharmaceutically relevant glycoproteins	456:521	four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B)	456:577	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	71	dep	glycoproteins	509:521	arg1	lactotransferrin					541:556	lactotransferrin	541:556	lactotransferrin	541:556	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	3	71	dep	glycoproteins	509:521	arg1	ribonuclease B					563:576	ribonuclease B	563:576	ribonuclease B	563:576	Using a set of four representative and biopharmaceutically relevant glycoproteins (IgG, fibrinogen, lactotransferrin, and ribonuclease B), the benefits and limitations of the developed workflow are highlighted and a state-of-the-art blueprint for conducting high-quality in-depth N- and O-glycoproteomic analyses is provided.					
30427586	6	72	theme	fragmentation	1140:1152	arg1	signature					1154:1162	this fragmentation signature	1135:1162	this fragmentation signature	1135:1162	It is shown for the first time that this fragmentation signature can consistently be found across all N-glycopeptides, but not on O-glycopeptides.					
30427586	2	73	theme	N-	405:406	arg1	analysis					393:400	the in-depth and site-specific analysis	362:400	the in-depth and site-specific analysis of N- and O-glycopeptides	362:426	Here, combining lower and stepped collisional energy fragmentation for the in-depth and site-specific analysis of N- and O-glycopeptides is proposed.					
30427586	7	74	theme	antenna	1447:1453	arg1	GlcNAc					1455:1460	antenna GlcNAc	1447:1460	antenna GlcNAc	1447:1460	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	7	75	theme	glycan	1318:1323	arg1	information					1335:1345	glycan structure information	1318:1345	glycan structure information	1318:1345	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	7	76	theme	bisecting	1466:1474	arg1	GlcNAc					1476:1481	bisecting GlcNAc	1466:1481	bisecting GlcNAc	1466:1481	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30427586	4	77	theme	extraction	882:891	arg1	version					800:806	a modified and improved version	776:806	a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment	776:919	Further, a modified and improved version of cotton hydrophilic interaction liquid chromatography-based solid phase extraction for glycopeptide enrichment is described.					
30427586	1	78	theme	mass	143:146	arg1	identification					162:175	unambiguous mass spectrometric identification	131:175	unambiguous mass spectrometric identification	131:175	The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro- and macroheterogeneity of glycoproteins.					
30427586	7	79	theme	structure	1325:1333	arg1	information					1335:1345	glycan structure information	1318:1345	glycan structure information	1318:1345	Moreover, the use of the relative abundance of oxonium ions to retrieve glycan structure information, for example, differentiation of hybrid- and high-mannose-type N-glycans or differentiation between antenna GlcNAc and bisecting GlcNAc, is systematically and comprehensively evaluated.					
30076319	2	0	theme	parasite	521:528	arg1	control					530:536	parasite control	521:536	parasite control	521:536	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	0	1	from	analyses	10:17	arg1	hepatica					75:82	the helminth Fasciola hepatica	53:82	the helminth Fasciola hepatica (liver fluke)	53:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	0	1	from	analyses	10:17	arg1	fluke					91:95	liver fluke	85:95	liver fluke	85:95	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	6	2	theme	simple	1373:1378	arg1	glycans					1380:1386	the structurally simple glycans	1356:1386	the structurally simple glycans described by F. hepatica glycomic datasets	1356:1429	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	0	3	theme	Fasciola	66:73	arg1	hepatica					75:82	the helminth Fasciola hepatica	53:82	the helminth Fasciola hepatica (liver fluke)	53:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	0	3	theme	Fasciola	66:73	arg1	fluke					91:95	liver fluke	85:95	liver fluke	85:95	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	7	4	theme	functional	1535:1544	arg1	interrogation					1554:1566	functional genomic interrogation	1535:1566	functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies	1535:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	4	5	from	putative roles	929:942	arg1	synthesis					980:988	nucleotide sugar synthesis	963:988	nucleotide sugar synthesis	963:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	5	from	putative roles	929:942	arg1	uptake					953:958	sugar uptake	947:958	sugar uptake	947:958	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	2	6	theme	parasitic	384:392	arg1	flatworms					394:402	parasitic flatworms	384:402	parasitic flatworms	384:402	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	7	7	with	interrogation	1554:1566	arg1	view					1595:1598	the view	1591:1598	the view towards novel parasite intervention strategies	1591:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	7	8	theme	novel	1608:1612	arg1	strategies					1636:1645	novel parasite intervention strategies	1608:1645	novel parasite intervention strategies	1608:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	0	9	theme	liver	85:89	arg1	hepatica					75:82	the helminth Fasciola hepatica	53:82	the helminth Fasciola hepatica (liver fluke)	53:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	0	9	theme	liver	85:89	arg1	fluke					91:95	liver fluke	85:95	liver fluke	85:95	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	7	10	theme	genomic	1546:1552	arg1	interrogation					1554:1566	functional genomic interrogation	1535:1566	functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies	1535:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	6	11	theme	wider	1484:1488	arg1	glycome					1490:1496	the wider glycome	1480:1496	the wider glycome	1480:1496	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	4	12	from	array	998:1002	arg1	synthesis					980:988	nucleotide sugar synthesis	963:988	nucleotide sugar synthesis	963:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	12	from	array	998:1002	arg1	uptake					953:958	sugar uptake	947:958	sugar uptake	947:958	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	1	13	theme	vaccine	289:295	arg1	preparations					297:308	vaccine preparations	289:308	vaccine preparations	289:308	Glycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations.					
30076319	4	14	theme	protein	1067:1073	arg1	N-					1075:1076	protein N-	1067:1076	protein N-	1067:1076	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	2	15	theme	poor	315:318	arg1	knowledge					320:328	Our poor knowledge	311:328	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms	311:402	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	4	16	theme	glycosidase	1031:1041	arg1	activities					1043:1052	glycosidase activities	1031:1052	glycosidase activities	1031:1052	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	1	17	theme	preparations	297:308	arg1	components					275:284	appealing components	265:284	appealing components of vaccine preparations	265:308	Glycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations.					
30076319	6	18	theme	glycomic	1413:1420	arg1	datasets					1422:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	3	19	theme	important	741:749	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	19	theme	important	741:749	arg1	humans					773:778	humans	773:778	humans	773:778	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	19	theme	important	741:749	arg1	livestock					790:798	their livestock	784:798	their livestock	784:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	19	theme	important	741:749	arg1	parasite					761:768	a globally important trematode parasite	730:768	a globally important trematode parasite of humans and their livestock	730:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	4	20	theme	F.	873:874	arg1	genomes					885:891	available F. hepatica genomes	863:891	available F. hepatica genomes	863:891	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	3	21	theme	first	559:563	arg1	description					574:584	the first detailed description	555:584	the first detailed description of genes associated with protein glycosylation in a parasitic flatworm	555:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	6	22	theme	O-linked	1301:1308	arg1	glycans					1310:1316	O-linked glycans	1301:1316	O-linked glycans	1301:1316	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	6	23	theme	hepatica	1404:1411	arg1	datasets					1422:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	4	24	theme	activities	1043:1052	arg1	putative roles					929:942	putative roles	929:942	putative roles in sugar uptake or nucleotide sugar synthesis	929:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	24	theme	activities	1043:1052	arg1	array					998:1002	an array	995:1002	an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation	995:1096	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	3	25	theme	detailed	565:572	arg1	description					574:584	the first detailed description	555:584	the first detailed description of genes associated with protein glycosylation in a parasitic flatworm	555:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	0	26	link	protein-linked	106:119	arg1	simplicity					128:137	protein-linked glycan simplicity	106:137	protein-linked glycan simplicity	106:137	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	4	27	theme	glycosyltransferase	1007:1025	arg1	putative roles					929:942	putative roles	929:942	putative roles in sugar uptake or nucleotide sugar synthesis	929:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	27	theme	glycosyltransferase	1007:1025	arg1	array					998:1002	an array	995:1002	an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation	995:1096	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	6	28	theme	structural	1455:1464	arg1	simplicity					1466:1475	pervasive structural simplicity	1445:1475	pervasive structural simplicity in the wider glycome	1445:1496	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	6	29	dep	F.	1228:1229	arg1	hepatica					1231:1238	F. hepatica	1228:1238	F. hepatica	1228:1238	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	0	30	theme	In	0:1	arg1	analyses					10:17	In silico analyses	0:17	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke)	0:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	5	31	theme	appreciable	1108:1118	arg1	duplication					1120:1130	appreciable duplication	1108:1130	appreciable duplication	1108:1130	We found appreciable duplication within these orthologues, describing just 87 non-redundant genes when paralogues were excluded.					
30076319	2	32	theme	pathways	337:344	arg1	knowledge					320:328	Our poor knowledge	311:328	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms	311:402	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	3	33	from	glycosylation	619:631	arg1	flatworm					648:655	a parasitic flatworm	636:655	a parasitic flatworm	636:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	4	34	dep	Using	801:805	arg1	genomes					885:891	available F. hepatica genomes	863:891	available F. hepatica genomes	863:891	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	35	dep	F.	873:874	arg1	hepatica					876:883	F. hepatica	873:883	available F. hepatica genomes	863:891	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	3	36	theme	humans	773:778	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	36	theme	humans	773:778	arg1	humans					773:778	humans	773:778	humans	773:778	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	36	theme	humans	773:778	arg1	livestock					790:798	their livestock	784:798	their livestock	784:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	36	theme	humans	773:778	arg1	parasite					761:768	a globally important trematode parasite	730:768	a globally important trematode parasite of humans and their livestock	730:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	0	37	theme	protein-linked	106:119	arg1	simplicity					128:137	protein-linked glycan simplicity	106:137	protein-linked glycan simplicity	106:137	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	6	38	theme	genomic	1338:1344	arg1	basis					1346:1350	the genomic basis	1334:1350	the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets	1334:1429	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	3	39	theme	genes	589:593	arg1	description					574:584	the first detailed description	555:584	the first detailed description of genes associated with protein glycosylation in a parasitic flatworm	555:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	6	40	link	O-linked	1301:1308	arg1	glycans					1310:1316	O-linked glycans	1301:1316	O-linked glycans	1301:1316	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	3	41	theme	protein	611:617	arg1	glycosylation					619:631	protein glycosylation	611:631	protein glycosylation in a parasitic flatworm	611:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	42	theme	trematode	751:759	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	42	theme	trematode	751:759	arg1	humans					773:778	humans	773:778	humans	773:778	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	42	theme	trematode	751:759	arg1	livestock					790:798	their livestock	784:798	their livestock	784:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	42	theme	trematode	751:759	arg1	parasite					761:768	a globally important trematode parasite	730:768	a globally important trematode parasite of humans and their livestock	730:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	4	43	theme	nucleotide	963:972	arg1	synthesis					980:988	nucleotide sugar synthesis	963:988	nucleotide sugar synthesis	963:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	1	44	theme	helminth	216:223	arg1	parasites					225:233	helminth parasites	216:233	helminth parasites	216:233	Glycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations.					
30076319	0	45	theme	glycosylating	30:42	arg1	genes					44:48	protein glycosylating genes	22:48	protein glycosylating genes	22:48	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	4	46	theme	sugar	974:978	arg1	synthesis					980:988	nucleotide sugar synthesis	963:988	nucleotide sugar synthesis	963:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	7	47	theme	parasite	1614:1621	arg1	strategies					1636:1645	novel parasite intervention strategies	1608:1645	novel parasite intervention strategies	1608:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	4	48	theme	search	830:835	arg1	sequences					817:825	190 human sequences	807:825	190 human sequences	807:825	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	48	theme	search	830:835	arg1	queries					837:843	search queries	830:843	search queries	830:843	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	0	49	theme	protein	22:28	arg1	genes					44:48	protein glycosylating genes	22:48	protein glycosylating genes	22:48	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	3	50	theme	livestock	790:798	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	50	theme	livestock	790:798	arg1	humans					773:778	humans	773:778	humans	773:778	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	50	theme	livestock	790:798	arg1	livestock					790:798	their livestock	784:798	their livestock	784:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	50	theme	livestock	790:798	arg1	parasite					761:768	a globally important trematode parasite	730:768	a globally important trematode parasite of humans and their livestock	730:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	0	51	theme	glycan	121:126	arg1	simplicity					128:137	protein-linked glycan simplicity	106:137	protein-linked glycan simplicity	106:137	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	3	52	theme	parasitic	638:646	arg1	flatworm					648:655	a parasitic flatworm	636:655	a parasitic flatworm	636:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	53	gly	glycosylation	619:631	arg1	flatworm					648:655	a parasitic flatworm	636:655	a parasitic flatworm	636:655	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	7	54	theme	pathways	1577:1584	arg1	interrogation					1554:1566	functional genomic interrogation	1535:1566	functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies	1535:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	2	55	gly	glycosylation	367:379	arg1	flatworms					394:402	parasitic flatworms	384:402	parasitic flatworms	384:402	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	6	56	theme	complex	1286:1292	arg1	N-					1294:1295	complex N-	1286:1295	complex N-	1286:1295	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	4	57	theme	sugar	947:951	arg1	uptake					953:958	sugar uptake	947:958	sugar uptake	947:958	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	7	58	theme	intervention	1623:1634	arg1	strategies					1636:1645	novel parasite intervention strategies	1608:1645	novel parasite intervention strategies	1608:1645	These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.					
30076319	0	59	theme	genes	44:48	arg1	analyses					10:17	In silico analyses	0:17	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke)	0:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	6	60	from	simplicity	1466:1475	arg1	glycome					1490:1496	the wider glycome	1480:1496	the wider glycome	1480:1496	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	6	61	theme	F.	1401:1402	arg1	datasets					1422:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica glycomic datasets	1401:1429	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	4	62	theme	available	863:871	arg1	genomes					885:891	available F. hepatica genomes	863:891	available F. hepatica genomes	863:891	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	63	theme	human	811:815	arg1	sequences					817:825	190 human sequences	807:825	190 human sequences	807:825	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	63	theme	human	811:815	arg1	queries					837:843	search queries	830:843	search queries	830:843	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	0	64	theme	expression	169:178	arg1	profiles					180:187	temporally-dynamic expression profiles	150:187	temporally-dynamic expression profiles	150:187	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	3	65	theme	Fasciola	701:708	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	65	theme	Fasciola	701:708	arg1	hepatica					710:717	Fasciola hepatica	701:717	Fasciola hepatica	701:717	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	2	66	theme	protein	359:365	arg1	glycosylation					367:379	protein glycosylation	359:379	protein glycosylation	359:379	Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control.					
30076319	4	67	with	identified 149	897:910	arg1	putative roles					929:942	putative roles	929:942	putative roles in sugar uptake or nucleotide sugar synthesis	929:988	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	4	67	with	identified 149	897:910	arg1	array					998:1002	an array	995:1002	an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation	995:1096	Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation.					
30076319	0	68	theme	temporally-dynamic	150:167	arg1	profiles					180:187	temporally-dynamic expression profiles	150:187	temporally-dynamic expression profiles	150:187	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	3	69	theme	liver	688:692	arg1	fluke					694:698	the liver fluke	684:698	the liver fluke (Fasciola hepatica)	684:718	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	69	theme	liver	688:692	arg1	hepatica					710:717	Fasciola hepatica	701:717	Fasciola hepatica	701:717	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	69	theme	liver	688:692	arg1	humans					773:778	humans	773:778	humans	773:778	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	69	theme	liver	688:692	arg1	livestock					790:798	their livestock	784:798	their livestock	784:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	3	69	theme	liver	688:692	arg1	parasite					761:768	a globally important trematode parasite	730:768	a globally important trematode parasite of humans and their livestock	730:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	0	70	gly	glycosylating	30:42	arg0	genes					44:48	protein glycosylating genes	22:48	protein glycosylating genes	22:48	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	0	71	theme	helminth	57:64	arg1	hepatica					75:82	the helminth Fasciola hepatica	53:82	the helminth Fasciola hepatica (liver fluke)	53:96	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	0	71	theme	helminth	57:64	arg1	fluke					91:95	liver fluke	85:95	liver fluke	85:95	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	3	72	theme	fluke	694:698	arg1	genome					674:679	the genome	670:679	the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock	670:798	Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock.					
30076319	6	73	theme	enzymes	1258:1264	arg1	many					1246:1249	many	1246:1249	many	1246:1249	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	6	73	theme	enzymes	1258:1264	arg1	enzymes					1258:1264	the enzymes	1254:1264	the enzymes	1254:1264	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	6	74	theme	pervasive	1445:1453	arg1	simplicity					1466:1475	pervasive structural simplicity	1445:1475	pervasive structural simplicity in the wider glycome	1445:1496	F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome.					
30076319	5	75	theme	non-redundant	1177:1189	arg1	genes					1191:1195	just 87 non-redundant genes	1169:1195	just 87 non-redundant genes	1169:1195	We found appreciable duplication within these orthologues, describing just 87 non-redundant genes when paralogues were excluded.					
30076319	0	76	dep	In	0:1	arg1	silico					3:8	silico	3:8	silico	3:8	In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.					
30076319	1	77	theme	appealing	265:273	arg1	components					275:284	appealing components	265:284	appealing components of vaccine preparations	265:308	Glycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations.					
30641224	6	0	attach	present	1070:1076	arg2	N-glycans					1060:1068	N-glycans	1060:1068	N-glycans present in HDL (~70%) and LDL (~60%)	1060:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	0	attach	present	1070:1076	arg1	HDL					1081:1083	HDL	1081:1083	HDL (~70%)	1081:1090	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	0	attach	present	1070:1076	arg1	LDL					1096:1098	LDL	1096:1098	LDL (~60%)	1096:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	0	attach	present	1070:1076	arg1	%					1104:1104	~60%	1101:1104	~60%	1101:1104	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	0	attach	present	1070:1076	arg1	%					1089:1089	~70%	1086:1089	~70%	1086:1089	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	4	1	theme	integrated	632:641	arg1	area					643:646	total integrated area	626:646	total integrated area	626:646	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	2	2	gly	N-glycosylation	295:309	arg1	glycomes					361:368	their glycomes	355:368	their glycomes	355:368	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	2	gly	N-glycosylation	295:309	arg1	metabolism					394:403	cellular cholesterol metabolism	373:403	cellular cholesterol metabolism	373:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	2	gly	N-glycosylation	295:309	arg1	LDL					335:337	LDL	335:337	LDL	335:337	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	2	gly	N-glycosylation	295:309	arg1	HDL					327:329	human plasma HDL	314:329	human plasma HDL	314:329	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	5	3	theme	N-glycans	945:953	arg1	analysis					921:928	HILIC-UHPLC-FLD analysis	905:928	HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F	905:974	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	11	4	theme	lipoproteins	1902:1913	arg1	properties					1884:1893	functional properties	1873:1893	functional properties of the lipoproteins	1873:1913	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	10	5	with	incubations	1682:1692	arg1	particles					1716:1724	desialylated LDL particles	1699:1724	desialylated LDL particles	1699:1724	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	2	6	from	role	347:350	arg1	metabolism					394:403	cellular cholesterol metabolism	373:403	cellular cholesterol metabolism	373:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	6	7	theme	sialic	1139:1144	arg1	residues					1151:1158	one or two sialic acid residues	1128:1158	one or two sialic acid residues	1128:1158	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	10	8	theme	Cellular	1592:1599	arg1	accumulation					1601:1612	Cellular accumulation	1592:1612	Cellular accumulation of cholesteryl esters in THP-1 cells	1592:1649	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	2	9	theme	plasma	320:325	arg1	HDL					327:329	human plasma HDL	314:329	human plasma HDL	314:329	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	4	10	from	peak	688:691	arg1	present					672:678	present	672:678	present	672:678	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	1	11	theme	various	182:188	arg1	structures					197:206	various glycan structures	182:206	various glycan structures whose composition and functional importance are starting to be recognized	182:280	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	9	12	from	efficacy	1489:1496	arg1	cholesterol					1519:1529	removing cellular cholesterol	1501:1529	removing cellular cholesterol from THP-1 cells	1501:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	9	12	from	efficacy	1489:1496	arg1	cells					1542:1546	THP-1 cells	1536:1546	THP-1 cells	1536:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	11	13	theme	plasma	1795:1800	arg1	lipoproteins					1802:1813	human plasma lipoproteins	1789:1813	human plasma lipoproteins	1789:1813	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	0	14	theme	functional	107:116	arg1	properties					118:127	their functional properties	101:127	their functional properties	101:127	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	8	15	theme	sialic	1424:1429	arg1	residues					1436:1443	sialic acid residues	1424:1443	sialic acid residues	1424:1443	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	10	16	theme	THP-1	1639:1643	arg1	cells					1645:1649	THP-1 cells	1639:1649	THP-1 cells	1639:1649	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	10	17	theme	esters	1629:1634	arg1	accumulation					1601:1612	Cellular accumulation	1592:1612	Cellular accumulation of cholesteryl esters in THP-1 cells	1592:1649	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	7	18	from	structure	1188:1196	arg1	LDL					1214:1216	LDL	1214:1216	LDL	1214:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	18	from	structure	1188:1196	arg1	HDL					1206:1208	HDL	1206:1208	HDL	1206:1208	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	11	19	theme	high	1825:1828	arg1	level					1830:1834	a high level	1823:1834	a high level	1823:1834	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	4	20	theme	cellular	757:764	arg1	efflux					766:771	cellular efflux	757:771	cellular efflux of cholesterol	757:786	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	5	21	theme	PNGase	967:972	arg1	F					974:974	PNGase F	967:974	PNGase F	967:974	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	2	22	from	N-glycosylation	295:309	arg1	metabolism					394:403	cellular cholesterol metabolism	373:403	cellular cholesterol metabolism	373:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	1	23	theme	plasma	141:146	arg1	lipoproteins					148:159	Human plasma lipoproteins	135:159	AIMS Human plasma lipoproteins	130:159	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	5	24	theme	HDL	933:935	arg1	N-glycans					945:953	HDL and LDL N-glycans	933:953	HDL and LDL N-glycans released by PNGase F	933:974	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	4	25	theme	Relative	519:526	arg1	abundance					528:536	Relative abundance	519:536	Relative abundance of the individual chromatographic peaks	519:576	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	25	theme	Relative	519:526	arg1	percentage					612:621	a percentage	610:621	a percentage of total integrated area	610:646	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	8	26	theme	Relative	1289:1296	arg1	abundances					1298:1307	Relative abundances	1289:1307	Relative abundances of several N-glycan structures	1289:1338	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	0	27	theme	diversity	67:75	arg1	level					58:62	a high level	51:62	a high level	51:62	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	5	28	theme	LDL	941:943	arg1	N-glycans					945:953	HDL and LDL N-glycans	933:953	HDL and LDL N-glycans released by PNGase F	933:974	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	9	29	from	cells	1542:1546	arg1	cholesterol					1519:1529	removing cellular cholesterol	1501:1529	removing cellular cholesterol from THP-1 cells	1501:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	9	29	from	cells	1542:1546	arg1	efficacy					1489:1496	significantly greater efficacy	1467:1496	significantly greater efficacy in removing cellular cholesterol from THP-1 cells	1467:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	11	30	theme	CONCLUSIONS	1764:1774	arg1	N-glycome					1776:1784	CONCLUSIONS N-glycome	1764:1784	CONCLUSIONS N-glycome of human plasma lipoproteins	1764:1813	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	9	31	theme	removing	1501:1508	arg1	cholesterol					1519:1529	removing cellular cholesterol	1501:1529	removing cellular cholesterol from THP-1 cells	1501:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	6	32	gly	sialylated	1112:1121	arg1	majority					1048:1055	The majority	1044:1055	The majority of N-glycans present in HDL (~70%) and LDL (~60%)	1044:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	8	33	theme	N-glycan	1320:1327	arg1	structures					1329:1338	several N-glycan structures	1312:1338	several N-glycan structures	1312:1338	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	7	34	theme	abundant	1170:1177	arg1	structure					1188:1196	The most abundant N-glycan structure	1161:1196	The most abundant N-glycan structure in both HDL and LDL	1161:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	34	theme	abundant	1170:1177	arg1	N-glycan					1249:1256	a complex type biantennary N-glycan	1222:1256	a complex type biantennary N-glycan	1222:1256	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	10	35	gly	desialylated	1699:1710	arg1	particles					1716:1724	desialylated LDL particles	1699:1724	desialylated LDL particles	1699:1724	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	5	36	theme	chromatographic	1007:1021	arg1	peaks					1023:1027	22 and 18 distinct chromatographic peaks	988:1027	22 and 18 distinct chromatographic peaks	988:1027	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	2	37	theme	cellular	373:380	arg1	metabolism					394:403	cellular cholesterol metabolism	373:403	cellular cholesterol metabolism	373:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	3	38	theme	LDL	478:480	arg1	profiles					425:432	METHODS N-glycomic profiles	406:432	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL	406:480	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	0	39	theme	human	17:21	arg1	lipoproteins					30:41	human plasma lipoproteins	17:41	human plasma lipoproteins	17:41	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	9	40	theme	cellular	1510:1517	arg1	cholesterol					1519:1529	removing cellular cholesterol	1501:1529	removing cellular cholesterol from THP-1 cells	1501:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	3	41	theme	N-glycomic	414:423	arg1	profiles					425:432	METHODS N-glycomic profiles	406:432	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL	406:480	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	1	42	contain	contain	174:180	arg2	structures					197:206	various glycan structures	182:206	various glycan structures whose composition and functional importance are starting to be recognized	182:280	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	1	42	contain	contain	174:180	arg1	AIMS					130:133	AIMS Human plasma lipoproteins	130:159	AIMS Human plasma lipoproteins	130:159	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	4	43	theme	cholesteryl	847:857	arg1	esters					859:864	cholesteryl esters	847:864	cholesteryl esters	847:864	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	3	44	theme	HDL	470:472	arg1	profiles					425:432	METHODS N-glycomic profiles	406:432	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL	406:480	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	0	45	theme	lipoproteins	30:41	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of human plasma lipoproteins	0:41	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	7	46	theme	sialic	1267:1272	arg1	acid					1274:1277	one sialic acid	1263:1277	one sialic acid (A2G2S1)	1263:1286	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	46	theme	sialic	1267:1272	arg1	A2G2S1					1280:1285	A2G2S1	1280:1285	A2G2S1	1280:1285	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	4	47	theme	individual	545:554	arg1	peaks					572:576	the individual chromatographic peaks	541:576	the individual chromatographic peaks	541:576	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	48	theme	esters	859:864	arg1	accumulation					831:842	cellular accumulation	822:842	cellular accumulation of cholesteryl esters	822:864	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	49	theme	peaks	572:576	arg1	abundance					528:536	Relative abundance	519:536	Relative abundance of the individual chromatographic peaks	519:576	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	49	theme	peaks	572:576	arg1	percentage					612:621	a percentage	610:621	a percentage of total integrated area	610:646	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	2	50	theme	LDL	335:337	arg1	N-glycosylation					295:309	N-glycosylation	295:309	N-glycosylation of human plasma HDL and LDL	295:337	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	50	theme	LDL	335:337	arg1	role					347:350	the role	343:350	the role of their glycomes in cellular cholesterol metabolism	343:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	7	51	theme	biantennary	1237:1247	arg1	structure					1188:1196	The most abundant N-glycan structure	1161:1196	The most abundant N-glycan structure in both HDL and LDL	1161:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	51	theme	biantennary	1237:1247	arg1	N-glycan					1249:1256	a complex type biantennary N-glycan	1222:1256	a complex type biantennary N-glycan	1222:1256	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	4	52	theme	area	643:646	arg1	abundance					528:536	Relative abundance	519:536	Relative abundance of the individual chromatographic peaks	519:576	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	52	theme	area	643:646	arg1	percentage					612:621	a percentage	610:621	a percentage of total integrated area	610:646	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	6	53	theme	present	1070:1076	arg1	N-glycans					1060:1068	N-glycans	1060:1068	N-glycans present in HDL (~70%) and LDL (~60%)	1060:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	54	from	LDL	1096:1098	arg1	present					1070:1076	present	1070:1076	present	1070:1076	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	2	55	theme	glycomes	361:368	arg1	N-glycosylation					295:309	N-glycosylation	295:309	N-glycosylation of human plasma HDL and LDL	295:337	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	55	theme	glycomes	361:368	arg1	role					347:350	the role	343:350	the role of their glycomes in cellular cholesterol metabolism	343:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	7	56	theme	complex	1224:1230	arg1	structure					1188:1196	The most abundant N-glycan structure	1161:1196	The most abundant N-glycan structure in both HDL and LDL	1161:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	56	theme	complex	1224:1230	arg1	N-glycan					1249:1256	a complex type biantennary N-glycan	1222:1256	a complex type biantennary N-glycan	1222:1256	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	9	57	gly	desialylated	1563:1574	arg1	HDL					1576:1578	desialylated HDL	1563:1578	desialylated HDL (p < 0.05)	1563:1589	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	9	57	gly	desialylated	1563:1574	arg1	p < 0.05					1581:1588	p < 0.05	1581:1588	p < 0.05	1581:1588	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	11	58	theme	functional	1873:1882	arg1	properties					1884:1893	functional properties	1873:1893	functional properties of the lipoproteins	1873:1913	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	10	59	from	accumulation	1601:1612	arg1	cells					1645:1649	THP-1 cells	1639:1649	THP-1 cells	1639:1649	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	4	60	theme	total	626:630	arg1	area					643:646	total integrated area	626:646	total integrated area	626:646	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	6	61	theme	acid	1146:1149	arg1	residues					1151:1158	one or two sialic acid residues	1128:1158	one or two sialic acid residues	1128:1158	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	7	62	with	N-glycan	1249:1256	arg1	acid					1274:1277	one sialic acid	1263:1277	one sialic acid (A2G2S1)	1263:1286	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	62	with	N-glycan	1249:1256	arg1	A2G2S1					1280:1285	A2G2S1	1280:1285	A2G2S1	1280:1285	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	6	63	from	present	1070:1076	arg1	HDL					1081:1083	HDL	1081:1083	HDL (~70%)	1081:1090	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	63	from	present	1070:1076	arg1	%					1089:1089	~70%	1086:1089	~70%	1086:1089	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	63	from	present	1070:1076	arg1	LDL					1096:1098	LDL	1096:1098	LDL (~60%)	1096:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	63	from	present	1070:1076	arg1	%					1104:1104	~60%	1101:1104	~60%	1101:1104	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	2	64	theme	HDL	327:329	arg1	N-glycosylation					295:309	N-glycosylation	295:309	N-glycosylation of human plasma HDL and LDL	295:337	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	2	64	theme	HDL	327:329	arg1	role					347:350	the role	343:350	the role of their glycomes in cellular cholesterol metabolism	343:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	5	65	theme	HILIC-UHPLC-FLD	905:919	arg1	analysis					921:928	HILIC-UHPLC-FLD analysis	905:928	HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F	905:974	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	4	66	theme	THP-1	884:888	arg1	cells					890:894	THP-1 cells	884:894	THP-1 cells	884:894	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	8	67	theme	neuraminidase	1373:1385	arg1	treatment					1387:1395	the neuraminidase treatment	1369:1395	the neuraminidase treatment	1369:1395	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	2	68	theme	human	314:318	arg1	HDL					327:329	human plasma HDL	314:329	human plasma HDL	314:329	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	1	69	theme	glycan	190:195	arg1	structures					197:206	various glycan structures	182:206	various glycan structures whose composition and functional importance are starting to be recognized	182:280	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	8	70	theme	acid	1431:1434	arg1	residues					1436:1443	sialic acid residues	1424:1443	sialic acid residues	1424:1443	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	9	71	theme	THP-1	1536:1540	arg1	cells					1542:1546	THP-1 cells	1536:1546	THP-1 cells	1536:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	4	72	theme	present	672:678	arg1	structures					661:670	N-glycan structures	652:670	N-glycan structures present in each peak	652:691	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	11	73	theme	lipoproteins	1802:1813	arg1	N-glycome					1776:1784	CONCLUSIONS N-glycome	1764:1784	CONCLUSIONS N-glycome of human plasma lipoproteins	1764:1813	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	4	74	from	present	672:678	arg1	peak					688:691	each peak	683:691	each peak	683:691	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	10	75	theme	cholesteryl	1617:1627	arg1	esters					1629:1634	cholesteryl esters	1617:1634	cholesteryl esters	1617:1634	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	11	76	theme	diversity	1839:1847	arg1	level					1830:1834	a high level	1823:1834	a high level	1823:1834	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	9	77	theme	greater	1481:1487	arg1	efficacy					1489:1496	significantly greater efficacy	1467:1496	significantly greater efficacy in removing cellular cholesterol from THP-1 cells	1467:1546	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	4	78	theme	HDL	742:744	arg1	capacity					730:737	The capacity	726:737	The capacity of HDL to mediate cellular efflux of cholesterol	726:786	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	78	theme	HDL	742:744	arg1	capacity					796:803	the capacity	792:803	the capacity of LDL to induce cellular accumulation of cholesteryl esters	792:864	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	6	79	from	HDL	1081:1083	arg1	present					1070:1076	present	1070:1076	present	1070:1076	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	6	80	theme	N-glycans	1060:1068	arg1	majority					1048:1055	The majority	1044:1055	The majority of N-glycans present in HDL (~70%) and LDL (~60%)	1044:1105	The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues.					
30641224	10	81	theme	LDL	1712:1714	arg1	particles					1716:1724	desialylated LDL particles	1699:1724	desialylated LDL particles	1699:1724	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	9	82	theme	Native	1446:1451	arg1	HDL					1453:1455	Native HDL	1446:1455	Native HDL	1446:1455	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	1	83	theme	Human	135:139	arg1	lipoproteins					148:159	Human plasma lipoproteins	135:159	AIMS Human plasma lipoproteins	130:159	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	11	84	theme	human	1789:1793	arg1	lipoproteins					1802:1813	human plasma lipoproteins	1789:1813	human plasma lipoproteins	1789:1813	CONCLUSIONS N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.					
30641224	7	85	theme	N-glycan	1179:1186	arg1	structure					1188:1196	The most abundant N-glycan structure	1161:1196	The most abundant N-glycan structure in both HDL and LDL	1161:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	85	theme	N-glycan	1179:1186	arg1	N-glycan					1249:1256	a complex type biantennary N-glycan	1222:1256	a complex type biantennary N-glycan	1222:1256	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	4	86	theme	N-glycan	652:659	arg1	structures					661:670	N-glycan structures	652:670	N-glycan structures present in each peak	652:691	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	1	87	dep	AIMS	130:133	arg1	lipoproteins					148:159	Human plasma lipoproteins	135:159	AIMS Human plasma lipoproteins	130:159	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	5	88	theme	distinct	998:1005	arg1	peaks					1023:1027	22 and 18 distinct chromatographic peaks	988:1027	22 and 18 distinct chromatographic peaks	988:1027	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	8	89	theme	several	1312:1318	arg1	structures					1329:1338	several N-glycan structures	1312:1338	several N-glycan structures	1312:1338	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	2	90	theme	cholesterol	382:392	arg1	metabolism					394:403	cellular cholesterol metabolism	373:403	cellular cholesterol metabolism	373:403	We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.					
30641224	9	91	theme	desialylated	1563:1574	arg1	HDL					1576:1578	desialylated HDL	1563:1578	desialylated HDL (p < 0.05)	1563:1589	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	9	91	theme	desialylated	1563:1574	arg1	p < 0.05					1581:1588	p < 0.05	1581:1588	p < 0.05	1581:1588	Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05).					
30641224	8	92	theme	structures	1329:1338	arg1	abundances					1298:1307	Relative abundances	1289:1307	Relative abundances of several N-glycan structures	1289:1338	Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues.					
30641224	3	93	theme	native	437:442	arg1	HDL					470:472	HDL	470:472	HDL	470:472	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	0	94	theme	plasma	23:28	arg1	lipoproteins					30:41	human plasma lipoproteins	17:41	human plasma lipoproteins	17:41	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	4	95	theme	LDL	808:810	arg1	capacity					730:737	The capacity	726:737	The capacity of HDL to mediate cellular efflux of cholesterol	726:786	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	95	theme	LDL	808:810	arg1	capacity					796:803	the capacity	792:803	the capacity of LDL to induce cellular accumulation of cholesteryl esters	792:864	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	96	theme	cholesterol	776:786	arg1	efflux					766:771	cellular efflux	757:771	cellular efflux of cholesterol	757:786	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	0	97	gly	Glycosylation	0:12	arg1	lipoproteins					30:41	human plasma lipoproteins	17:41	human plasma lipoproteins	17:41	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	4	98	theme	chromatographic	556:570	arg1	peaks					572:576	the individual chromatographic peaks	541:576	the individual chromatographic peaks	541:576	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	0	99	theme	high	53:56	arg1	level					58:62	a high level	51:62	a high level	51:62	Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties.					
30641224	3	100	theme	METHODS	406:412	arg1	profiles					425:432	METHODS N-glycomic profiles	406:432	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL	406:480	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	4	101	attach	present	672:678	arg2	structures					661:670	N-glycan structures	652:670	N-glycan structures present in each peak	652:691	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	4	101	attach	present	672:678	arg1	peak					688:691	each peak	683:691	each peak	683:691	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	5	102	dep	RESULTS	897:903	arg1	resulted					976:983	resulted	976:983	resulted	976:983	RESULTS HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively.					
30641224	7	103	theme	type	1232:1235	arg1	structure					1188:1196	The most abundant N-glycan structure	1161:1196	The most abundant N-glycan structure in both HDL and LDL	1161:1216	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	7	103	theme	type	1232:1235	arg1	N-glycan					1249:1256	a complex type biantennary N-glycan	1222:1256	a complex type biantennary N-glycan	1222:1256	The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1).					
30641224	10	104	theme	desialylated	1699:1710	arg1	particles					1716:1724	desialylated LDL particles	1699:1724	desialylated LDL particles	1699:1724	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	1	105	theme	functional	230:239	arg1	importance					241:250	functional importance	230:250	functional importance	230:250	AIMS Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized.					
30641224	3	106	theme	neuraminidase-treated	448:468	arg1	HDL					470:472	HDL	470:472	HDL	470:472	METHODS N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD.					
30641224	4	107	theme	cellular	822:829	arg1	accumulation					831:842	cellular accumulation	822:842	cellular accumulation of cholesteryl esters	822:864	Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.					
30641224	10	108	theme	native	1741:1746	arg1	p < 0.05					1753:1760	p < 0.05	1753:1760	p < 0.05	1753:1760	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
30641224	10	108	theme	native	1741:1746	arg1	LDL					1748:1750	native LDL	1741:1750	native LDL (p < 0.05)	1741:1761	Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).					
31213534	7	0	theme	cancer-promoting	1515:1530	arg1	activity					1532:1539	the cancer-promoting activity	1511:1539	the cancer-promoting activity of β-galactose-binding galectins	1511:1572	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	5	1	theme	cancer-associated	968:984	arg1	modifications					993:1005	cancer-associated glycan modifications	968:1005	cancer-associated glycan modifications	968:1005	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	2	2	theme	metastases	355:364	arg1	formation					366:374	metastases formation	355:374	metastases formation	355:374	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	5	3	theme	glycome	1192:1198	arg1	role					1180:1183	the role	1176:1183	the role of the glycome in malignant progression	1176:1223	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	7	4	theme	exciting	1448:1455	arg1	insights					1457:1464	exciting insights	1448:1464	exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins	1448:1572	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	4	5	theme	progression	867:877	arg1	biomarkers					845:854	biomarkers	845:854	biomarkers of disease progression	845:877	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	3	6	theme	lectins	575:581	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	7	theme	other	588:592	arg1	proteins					615:622	other type-1 transmembrane proteins	588:622	other type-1 transmembrane proteins	588:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	5	8	theme	reenergized	1144:1154	arg1	investigations					1156:1169	reenergized investigations	1144:1169	reenergized investigations into the role of the glycome in malignant progression	1144:1223	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	9	theme	group	1031:1035	arg1	antigens					1039:1046	blood group I antigens	1025:1046	blood group I antigens	1025:1046	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	10	theme	modifications	993:1005	arg1	focus					959:963	the focus	955:963	the focus of cancer-associated glycan modifications	955:1005	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	1	11	theme	Cell	73:76	arg1	effectors					152:160	ubiquitous posttranslational effectors	123:160	ubiquitous posttranslational effectors that can tune cancer progression	123:193	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	1	11	theme	Cell	73:76	arg1	carbohydrates					86:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	6	12	theme	I-branched	1226:1235	arg1	glycans					1237:1243	I-branched glycans	1226:1243	I-branched glycans catalyzed principally by the I-branching enzyme GCNT2	1226:1297	I-branched glycans catalyzed principally by the I-branching enzyme GCNT2 are now indicated in several malignancies.					
31213534	3	13	theme	growth	521:526	arg1	receptors					535:543	growth factor receptors	521:543	growth factor receptors	521:543	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	4	14	theme	preclinical	740:750	arg1	studies					752:758	preclinical studies	740:758	preclinical studies on the suitability of glycans as anticancer therapeutic targets	740:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	2	15	from	transformation	337:350	arg1	steps					315:319	essentially all progressive steps	287:319	essentially all progressive steps	287:319	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	1	16	theme	surface	78:84	arg1	effectors					152:160	ubiquitous posttranslational effectors	123:160	ubiquitous posttranslational effectors that can tune cancer progression	123:193	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	1	16	theme	surface	78:84	arg1	carbohydrates					86:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	7	17	theme	GCNT2/I-branching	1384:1400	arg1	role					1376:1379	the putative role	1363:1379	the putative role of GCNT2/I-branching in cancer progression	1363:1422	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	4	18	theme	malignant	682:690	arg1	activity					692:699	a cancer cell's malignant activity	666:699	a cancer cell's malignant activity	666:699	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	7	19	theme	β-galactose-binding	1544:1562	arg1	galectins					1564:1572	β-galactose-binding galectins	1544:1572	β-galactose-binding galectins	1544:1572	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	4	20	theme	therapeutic	804:814	arg1	targets					816:822	anticancer therapeutic targets	793:822	anticancer therapeutic targets	793:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	1	21	theme	cancer	176:181	arg1	progression					183:193	cancer progression	176:193	cancer progression	176:193	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	5	22	contain	has	1140:1142	arg1	emergence					1012:1020	the emergence	1008:1020	the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence	1008:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	22	contain	has	1140:1142	arg2	investigations					1156:1169	reenergized investigations	1144:1169	reenergized investigations into the role of the glycome in malignant progression	1144:1223	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	7	23	theme	galectins	1564:1572	arg1	activity					1532:1539	the cancer-promoting activity	1511:1539	the cancer-promoting activity of β-galactose-binding galectins	1511:1572	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	6	24	theme	several	1320:1326	arg1	malignancies					1328:1339	several malignancies	1320:1339	several malignancies	1320:1339	I-branched glycans catalyzed principally by the I-branching enzyme GCNT2 are now indicated in several malignancies.					
31213534	3	25	theme	type-1	594:599	arg1	proteins					615:622	other type-1 transmembrane proteins	588:622	other type-1 transmembrane proteins	588:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	26	theme	glycan-binding	445:458	arg1	proteins					460:467	promalignant glycan-binding proteins	432:467	promalignant glycan-binding proteins	432:467	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	0	27	theme	I-branched	0:9	arg1	carbohydrates					11:23	I-branched carbohydrates	0:23	I-branched carbohydrates as emerging effectors of malignant progression	0:70	I-branched carbohydrates as emerging effectors of malignant progression.					
31213534	3	28	theme	transmembrane	601:613	arg1	proteins					615:622	other type-1 transmembrane proteins	588:622	other type-1 transmembrane proteins	588:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	0	29	theme	emerging	28:35	arg1	effectors					37:45	emerging effectors	28:45	emerging effectors of malignant progression	28:70	I-branched carbohydrates as emerging effectors of malignant progression.					
31213534	5	30	theme	I-branched	1052:1061	arg1	glycans					1063:1069	I-branched glycans	1052:1069	I-branched glycans	1052:1069	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	31	theme	cell	1079:1082	arg1	moieties					1092:1099	key cell surface moieties	1075:1099	key cell surface moieties capable of modulating cancer virulence	1075:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	2	32	theme	progressive	303:313	arg1	steps					315:319	essentially all progressive steps	287:319	essentially all progressive steps	287:319	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	3	33	theme	receptor-ligand	493:507	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	34	theme	structural	389:398	arg1	entities					400:407	structural entities	389:407	structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	389:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	34	theme	structural	389:398	arg1	Glycans					377:383	Glycans	377:383	Glycans	377:383	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	35	theme	ligands	566:572	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	36	theme	integrins	546:554	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	5	37	theme	key	1075:1077	arg1	moieties					1092:1099	key cell surface moieties	1075:1099	key cell surface moieties capable of modulating cancer virulence	1075:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	7	38	theme	putative	1367:1374	arg1	role					1376:1379	the putative role	1363:1379	the putative role of GCNT2/I-branching in cancer progression	1363:1422	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	5	39	theme	blood	1025:1029	arg1	antigens					1039:1046	blood group I antigens	1025:1046	blood group I antigens	1025:1046	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	40	theme	cancer	1123:1128	arg1	virulence					1130:1138	cancer virulence	1123:1138	cancer virulence	1123:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	3	41	theme	promalignant	432:443	arg1	proteins					460:467	promalignant glycan-binding proteins	432:467	promalignant glycan-binding proteins	432:467	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	7	42	theme	cancer	1405:1410	arg1	progression					1412:1422	cancer progression	1405:1422	cancer progression	1405:1422	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	2	43	theme	atypical	235:242	arg1	levels					244:249	atypical levels	235:249	atypical levels	235:249	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	3	44	theme	optimal	485:491	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	0	45	theme	progression	60:70	arg1	effectors					37:45	emerging effectors	28:45	emerging effectors of malignant progression	28:70	I-branched carbohydrates as emerging effectors of malignant progression.					
31213534	5	46	theme	malignant	1203:1211	arg1	progression					1213:1223	malignant progression	1203:1223	malignant progression	1203:1223	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	3	47	theme	proteins	615:622	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	3	48	theme	factor	528:533	arg1	receptors					535:543	growth factor receptors	521:543	growth factor receptors	521:543	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	0	49	theme	malignant	50:58	arg1	progression					60:70	malignant progression	50:70	malignant progression	50:70	I-branched carbohydrates as emerging effectors of malignant progression.					
31213534	5	50	theme	surface	1084:1090	arg1	moieties					1092:1099	key cell surface moieties	1075:1099	key cell surface moieties capable of modulating cancer virulence	1075:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	4	51	theme	disease	859:865	arg1	progression					867:877	disease progression	859:877	disease progression	859:877	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	7	52	from	role	1376:1379	arg1	progression					1412:1422	cancer progression	1405:1422	cancer progression	1405:1422	In this Perspective, the putative role of GCNT2/I-branching in cancer progression is discussed, including exciting insights on how I-branches can potentially antagonize the cancer-promoting activity of β-galactose-binding galectins.					
31213534	3	53	theme	receptors	535:543	arg1	activity					509:516	optimal receptor-ligand activity	485:516	optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins	485:622	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	6	54	theme	enzyme	1286:1291	arg1	GCNT2					1293:1297	the I-branching enzyme GCNT2	1270:1297	the I-branching enzyme GCNT2	1270:1297	I-branched glycans catalyzed principally by the I-branching enzyme GCNT2 are now indicated in several malignancies.					
31213534	2	55	theme	malignant	327:335	arg1	transformation					337:350	malignant transformation	327:350	malignant transformation	327:350	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	4	56	theme	glycans	782:788	arg1	suitability					767:777	the suitability	763:777	the suitability of glycans as anticancer therapeutic targets	763:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	5	57	theme	antigens	1039:1046	arg1	emergence					1012:1020	the emergence	1008:1020	the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence	1008:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	58	theme	capable	1101:1107	arg1	moieties					1092:1099	key cell surface moieties	1075:1099	key cell surface moieties capable of modulating cancer virulence	1075:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	4	59	theme	anticancer	793:802	arg1	targets					816:822	anticancer therapeutic targets	793:822	anticancer therapeutic targets	793:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	1	60	theme	ubiquitous	123:132	arg1	effectors					152:160	ubiquitous posttranslational effectors	123:160	ubiquitous posttranslational effectors that can tune cancer progression	123:193	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	1	60	theme	ubiquitous	123:132	arg1	carbohydrates					86:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	6	61	theme	I-branching	1274:1284	arg1	GCNT2					1293:1297	the I-branching enzyme GCNT2	1270:1297	the I-branching enzyme GCNT2	1270:1297	I-branched glycans catalyzed principally by the I-branching enzyme GCNT2 are now indicated in several malignancies.					
31213534	5	62	theme	glycan	986:991	arg1	modifications					993:1005	cancer-associated glycan modifications	968:1005	cancer-associated glycan modifications	968:1005	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	4	63	theme	cancer	668:673	arg1	cell					675:678	a cancer cell's	666:680	a cancer cell's malignant activity	666:699	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	2	64	theme	cancer	254:259	arg1	cells					261:265	cancer cells	254:265	cancer cells	254:265	Often aberrantly displayed or found at atypical levels on cancer cells, glycans can impact essentially all progressive steps, from malignant transformation to metastases formation.					
31213534	4	65	from	promise	834:840	arg1	suitability					767:777	the suitability	763:777	the suitability of glycans as anticancer therapeutic targets	763:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	1	66	theme	posttranslational	134:150	arg1	effectors					152:160	ubiquitous posttranslational effectors	123:160	ubiquitous posttranslational effectors that can tune cancer progression	123:193	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	1	66	theme	posttranslational	134:150	arg1	carbohydrates					86:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates	73:98	Cell surface carbohydrates, termed "glycans," are ubiquitous posttranslational effectors that can tune cancer progression.					
31213534	4	67	from	studies	752:758	arg1	suitability					767:777	the suitability	763:777	the suitability of glycans as anticancer therapeutic targets	763:822	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
31213534	5	68	from	role	1180:1183	arg1	progression					1213:1223	malignant progression	1203:1223	malignant progression	1203:1223	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	5	69	theme	glycans	1063:1069	arg1	emergence					1012:1020	the emergence	1008:1020	the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence	1008:1138	While sialylation and fucosylation have predominated the focus of cancer-associated glycan modifications, the emergence of blood group I antigens (or I-branched glycans) as key cell surface moieties capable of modulating cancer virulence has reenergized investigations into the role of the glycome in malignant progression.					
31213534	3	70	theme	integrin	557:564	arg1	ligands					566:572	integrin ligands	557:572	integrin ligands	557:572	Glycans are structural entities that can directly bind promalignant glycan-binding proteins and help elicit optimal receptor-ligand activity of growth factor receptors, integrins, integrin ligands, lectins, and other type-1 transmembrane proteins.					
31213534	4	71	theme	integral	649:656	arg1	role					658:661	an integral role	646:661	an integral role	646:661	Because glycans play an integral role in a cancer cell's malignant activity and are frequently uniquely expressed, preclinical studies on the suitability of glycans as anticancer therapeutic targets and their promise as biomarkers of disease progression continue to intensify.					
29716988	10	0	theme	N-glycan	1907:1914	arg1	moieties					1916:1923	N-glycan moieties	1907:1923	N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances	1907:2049	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	1	1	theme	peptides	244:251	arg1	myriad					201:206	a myriad	199:206	a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation	199:373	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	4	2	theme	methylated	923:932	arg1	analogs					934:940	methylated analogs	923:940	methylated analogs using ion-trap mass spectrometry (MSn)	923:979	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	6	3	theme	Complex	1231:1237	arg1	venoms					1208:1213	the venoms	1204:1213	the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca)	1204:1282	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	7	4	theme	relative	1453:1460	arg1	abundances					1462:1471	variable relative abundances	1444:1471	variable relative abundances in the venoms	1444:1485	Further, quantitative analysis of these N-glycans showed variable relative abundances in the venoms.					
29716988	7	5	theme	quantitative	1396:1407	arg1	analysis					1409:1416	quantitative analysis	1396:1416	quantitative analysis of these N-glycans	1396:1435	Further, quantitative analysis of these N-glycans showed variable relative abundances in the venoms.					
29716988	10	6	theme	Bothrops	2122:2129	arg1	features					2139:2146	features	2139:2146	features that could dramatically contribute to their distinct biological activities	2139:2221	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	10	6	theme	Bothrops	2122:2129	arg1	venoms					2131:2136	Bothrops venoms	2122:2136	Bothrops venoms	2122:2136	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	3	7	theme	phylogenetic	723:734	arg1	classification					736:749	their phylogenetic classification	717:749	their phylogenetic classification	717:749	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	8	8	from	venoms	1567:1572	arg1	present					1550:1556	present	1550:1556	present	1550:1556	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	10	9	located	found	1935:1939	arg1	structures					2015:2024	isomer structures	2008:2024	isomer structures	2008:2024	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	10	9	located	found	1935:1939	arg1	glycoproteins					1944:1956	glycoproteins	1944:1956	glycoproteins	1944:1956	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	10	9	located	found	1935:1939	arg1	abundances					2040:2049	variable abundances	2031:2049	variable abundances	2031:2049	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	10	9	located	found	1935:1939	arg2	moieties					1916:1923	N-glycan moieties	1907:1923	N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances	1907:2049	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	1	10	used	used	262:265	arg2	peptides					244:251	peptides	244:251	peptides	244:251	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	1	10	used	used	262:265	arg2	proteins					231:238	biologically active proteins	211:238	biologically active proteins	211:238	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	10	11	theme	complexity	2108:2117	arg1	level					2099:2103	another level	2091:2103	another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities	2091:2221	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	6	12	dep	venoms	1208:1213	arg1	alcatraz					1243:1250	B. alcatraz	1240:1250	B. alcatraz	1240:1250	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	6	12	dep	venoms	1208:1213	arg1	insularis					1256:1264	B. insularis	1253:1264	B. insularis	1253:1264	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	6	12	dep	venoms	1208:1213	arg1	jararaca					1274:1281	B. jararaca	1271:1281	B. jararaca	1271:1281	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	0	13	theme	Viperid	114:120	arg1	Snakes					122:127	Viperid Snakes	114:127	Viperid Snakes	114:127	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	6	14	dep	moojeni	1327:1333	arg1	venoms					1351:1356	neuwiedi venoms	1342:1356	neuwiedi venoms	1342:1356	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	9	15	theme	phylogeny	1690:1698	arg1	cladograms					1700:1709	the phylogeny cladograms	1686:1709	the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits	1686:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	10	16	theme	isomer	2008:2013	arg1	structures					2015:2024	isomer structures	2008:2024	isomer structures	2008:2024	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	4	17	theme	mass	957:960	arg1	MSn					976:978	MSn	976:978	MSn	976:978	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	4	17	theme	mass	957:960	arg1	spectrometry					962:973	ion-trap mass spectrometry	948:973	ion-trap mass spectrometry (MSn)	948:979	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	10	18	theme	branching	1997:2005	arg1	degrees					1986:1992	different degrees	1976:1992	different degrees of branching	1976:2005	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	5	19	theme	unique	1100:1105	arg1	compositions					1107:1118	52 unique compositions	1097:1118	52 unique compositions in Bothrops venoms	1097:1137	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	9	20	theme	American	1720:1727	arg1	snakes					1740:1745	South American bothropoid snakes	1714:1745	South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits	1714:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	0	21	from	Phenotype	101:109	arg1	Snakes					122:127	Viperid Snakes	114:127	Viperid Snakes	114:127	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	10	22	theme	variable	2031:2038	arg1	abundances					2040:2049	variable abundances	2031:2049	variable abundances	2031:2049	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	9	23	dep	morphological	1770:1782	arg1	molecular					1785:1793	molecular	1785:1793	molecular	1785:1793	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	9	24	theme	snakes	1740:1745	arg1	cladograms					1700:1709	the phylogeny cladograms	1686:1709	the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits	1686:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	6	25	theme	B.	1369:1370	arg1	venom					1380:1384	B. cotiara venom	1369:1384	B. cotiara venom	1369:1384	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	3	26	theme	venom	615:619	arg1	glycoproteomes					621:634	Bothrops venom glycoproteomes	606:634	Bothrops venom glycoproteomes	606:634	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	9	27	theme	bothropoid	1729:1738	arg1	snakes					1740:1745	South American bothropoid snakes	1714:1745	South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits	1714:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	5	28	from	compositions	1107:1118	arg1	venoms					1132:1137	Bothrops venoms	1123:1137	Bothrops venoms	1123:1137	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	1	29	located	observed	350:357	arg1	envenomation					362:373	envenomation	362:373	envenomation	362:373	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	1	29	located	observed	350:357	arg2	effects					342:348	the pathological effects	325:348	the pathological effects observed on envenomation	325:373	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	1	30	theme	pathological	329:340	arg1	effects					342:348	the pathological effects	325:348	the pathological effects observed on envenomation	325:373	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	5	31	theme	multiple	1016:1023	arg1	steps					1025:1029	multiple steps	1016:1029	multiple steps	1016:1029	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	2	32	theme	proteomes	562:570	arg1	variation					543:551	the variation	539:551	the variation of venom proteomes	539:570	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	6	33	theme	neuwiedi	1342:1349	arg1	venoms					1351:1356	neuwiedi venoms	1342:1356	neuwiedi venoms	1342:1356	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	8	34	theme	comprehensive	1509:1521	arg1	structures					1539:1548	N-glycan structures	1530:1548	N-glycan structures present in snake venoms	1530:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	8	34	theme	comprehensive	1509:1521	arg1	set					1523:1525	a comprehensive set	1507:1525	a comprehensive set of N-glycan structures present in snake venoms	1507:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	6	35	dep	B.	1369:1370	arg1	cotiara					1372:1378	cotiara	1372:1378	cotiara	1372:1378	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	5	36	theme	Following	982:990	arg1	disassembly					996:1006	Following ion disassembly	982:1006	Following ion disassembly through multiple steps	982:1029	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	9	37	theme	feeding	1804:1810	arg1	habits					1812:1817	feeding habits	1804:1817	feeding habits	1804:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	0	38	theme	N-Glycans	14:22	arg1	Structures					0:9	Structures	0:9	Structures of N-Glycans of Bothrops Venoms	0:41	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	8	39	theme	structures	1539:1548	arg1	structures					1539:1548	N-glycan structures	1530:1548	N-glycan structures present in snake venoms	1530:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	8	39	theme	structures	1539:1548	arg1	set					1523:1525	a comprehensive set	1507:1525	a comprehensive set of N-glycan structures present in snake venoms	1507:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	3	40	theme	components	654:663	arg1	core					646:649	a core	644:649	a core of components that markedly define their composition and parallel their phylogenetic classification	644:749	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	2	41	theme	main	397:400	arg1	Glycosylation					376:388	Glycosylation	376:388	Glycosylation	376:388	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	41	theme	main	397:400	arg1	PTM					435:437	PTM	435:437	PTM	435:437	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	41	theme	main	397:400	arg1	modification					421:432	the main post-translational modification	393:432	the main post-translational modification (PTM) of viperid venoms	393:456	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	0	42	theme	Bothrops	27:34	arg1	Venoms					36:41	Bothrops Venoms	27:41	Bothrops Venoms	27:41	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	2	43	theme	venoms	451:456	arg1	Glycosylation					376:388	Glycosylation	376:388	Glycosylation	376:388	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	43	theme	venoms	451:456	arg1	PTM					435:437	PTM	435:437	PTM	435:437	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	43	theme	venoms	451:456	arg1	modification					421:432	the main post-translational modification	393:432	the main post-translational modification (PTM) of viperid venoms	393:456	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	0	44	theme	Molecular	55:63	arg1	Signatures					65:74	Molecular Signatures	55:74	Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes	55:127	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	4	45	theme	cartoon	859:865	arg1	structures					867:876	assigned cartoon structures	850:876	assigned cartoon structures	850:876	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	1	46	theme	active	224:229	arg1	proteins					231:238	biologically active proteins	211:238	biologically active proteins	211:238	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	9	47	theme	distinct	1831:1838	arg1	signatures					1850:1859	distinct molecular signatures	1831:1859	distinct molecular signatures for each venom	1831:1874	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	5	48	theme	isomeric	1061:1068	arg1	details					1070:1076	sequence and linkage isomeric details	1040:1076	details	1070:1076	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	10	49	theme	moieties	1916:1923	arg1	complexity					1893:1902	the complexity	1889:1902	the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances	1889:2049	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	8	50	from	present	1550:1556	arg1	venoms					1567:1572	snake venoms	1561:1572	snake venoms	1561:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	10	51	theme	distinct	2192:2199	arg1	activities					2212:2221	their distinct biological activities	2186:2221	their distinct biological activities	2186:2221	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	7	52	theme	variable	1444:1451	arg1	abundances					1462:1471	variable relative abundances	1444:1471	variable relative abundances in the venoms	1444:1485	Further, quantitative analysis of these N-glycans showed variable relative abundances in the venoms.					
29716988	6	53	theme	Jararaca	1222:1229	arg1	Complex					1231:1237	the Jararaca Complex	1218:1237	the Jararaca Complex	1218:1237	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	2	54	theme	viperid	443:449	arg1	venoms					451:456	viperid venoms	443:456	viperid venoms	443:456	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	7	55	theme	N-glycans	1427:1435	arg1	analysis					1409:1416	quantitative analysis	1396:1416	quantitative analysis of these N-glycans	1396:1435	Further, quantitative analysis of these N-glycans showed variable relative abundances in the venoms.					
29716988	9	56	theme	venoms	1672:1677	arg1	N-glycomes					1652:1661	the N-glycomes	1648:1661	the N-glycomes of these venoms	1648:1677	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	10	57	theme	different	1976:1984	arg1	degrees					1986:1992	different degrees	1976:1992	different degrees of branching	1976:2005	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	2	58	theme	venom	556:560	arg1	proteomes					562:570	venom proteomes	556:570	venom proteomes	556:570	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	1	59	theme	venoms	154:159	arg1	complexity					134:143	The complexity	130:143	The complexity of snake venoms	130:159	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	10	60	gly	glycoproteins	1944:1956	arg1	glycoproteins					1944:1956	glycoproteins	1944:1956	glycoproteins	1944:1956	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	8	61	attach	present	1550:1556	arg1	venoms					1567:1572	snake venoms	1561:1572	snake venoms	1561:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	8	61	attach	present	1550:1556	arg2	structures					1539:1548	N-glycan structures	1530:1548	N-glycan structures present in snake venoms	1530:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	4	62	theme	ion-trap	948:955	arg1	MSn					976:978	MSn	976:978	MSn	976:978	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	4	62	theme	ion-trap	948:955	arg1	spectrometry					962:973	ion-trap mass spectrometry	948:973	ion-trap mass spectrometry (MSn)	948:979	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	5	63	theme	Bothrops	1123:1130	arg1	venoms					1132:1137	Bothrops venoms	1123:1137	Bothrops venoms	1123:1137	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	3	64	theme	Bothrops	606:613	arg1	glycoproteomes					621:634	Bothrops venom glycoproteomes	606:634	Bothrops venom glycoproteomes	606:634	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	4	65	dep	species	804:810	arg1	detailing					882:890	detailing	882:890	detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn)	882:979	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	4	65	dep	species	804:810	arg1	evaluating					812:821	evaluating	812:821	evaluating the N-glycomes by LC-MS as assigned cartoon structures	812:876	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	2	66	theme	little	481:486	arg1	understanding					488:500	little understanding	481:500	little understanding of how protein glycosylation impacts the variation of venom proteomes	481:570	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	10	67	theme	biological	2201:2210	arg1	activities					2212:2221	their distinct biological activities	2186:2221	their distinct biological activities	2186:2221	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	9	68	theme	morphological	1770:1782	arg1	data					1795:1798	morphological, molecular data	1770:1798	morphological, molecular data	1770:1798	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	6	69	from	22	1363:1364	arg1	venom					1380:1384	B. cotiara venom	1369:1384	B. cotiara venom	1369:1384	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	1	70	theme	snake	148:152	arg1	venoms					154:159	snake venoms	148:159	snake venoms	148:159	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
29716988	8	71	theme	first	1496:1500	arg1	time					1502:1505	the first time	1492:1505	the first time	1492:1505	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	0	72	theme	Venom	95:99	arg1	Phenotype					101:109	Venom Phenotype	95:109	Venom Phenotype in Viperid Snakes	95:127	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	2	73	theme	protein	509:515	arg1	glycosylation					517:529	protein glycosylation	509:529	protein glycosylation	509:529	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	7	74	from	abundances	1462:1471	arg1	venoms					1480:1485	the venoms	1476:1485	the venoms	1476:1485	Further, quantitative analysis of these N-glycans showed variable relative abundances in the venoms.					
29716988	4	75	theme	Bothrops	795:802	arg1	species					804:810	eight Bothrops species	789:810	eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn)	789:979	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	8	76	theme	N-glycan	1530:1537	arg1	structures					1539:1548	N-glycan structures	1530:1548	N-glycan structures present in snake venoms	1530:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	5	77	theme	ion	992:994	arg1	disassembly					996:1006	Following ion disassembly	982:1006	Following ion disassembly through multiple steps	982:1029	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	8	78	theme	present	1550:1556	arg1	structures					1539:1548	N-glycan structures	1530:1548	N-glycan structures present in snake venoms	1530:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	10	79	from	level	2099:2103	arg1	features					2139:2146	features	2139:2146	features that could dramatically contribute to their distinct biological activities	2139:2221	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	10	79	from	level	2099:2103	arg1	venoms					2131:2136	Bothrops venoms	2122:2136	Bothrops venoms	2122:2136	Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.					
29716988	2	80	theme	post-translational	402:419	arg1	Glycosylation					376:388	Glycosylation	376:388	Glycosylation	376:388	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	80	theme	post-translational	402:419	arg1	PTM					435:437	PTM	435:437	PTM	435:437	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	2	80	theme	post-translational	402:419	arg1	modification					421:432	the main post-translational modification	393:432	the main post-translational modification (PTM) of viperid venoms	393:456	Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes.					
29716988	0	81	theme	Venoms	36:41	arg1	N-Glycans					14:22	N-Glycans	14:22	N-Glycans of Bothrops Venoms	14:41	Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.					
29716988	8	82	theme	snake	1561:1565	arg1	venoms					1567:1572	snake venoms	1561:1572	snake venoms	1561:1572	For the first time a comprehensive set of N-glycan structures present in snake venoms are defined.					
29716988	9	83	theme	molecular	1840:1848	arg1	signatures					1850:1859	distinct molecular signatures	1831:1859	distinct molecular signatures for each venom	1831:1874	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	4	84	theme	assigned	850:857	arg1	structures					867:876	assigned cartoon structures	850:876	assigned cartoon structures	850:876	Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn).					
29716988	9	85	from	studies	1759:1765	arg1	data					1795:1798	morphological, molecular data	1770:1798	morphological, molecular data	1770:1798	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	9	85	from	studies	1759:1765	arg1	habits					1812:1817	feeding habits	1804:1817	feeding habits	1804:1817	Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom.					
29716988	5	86	theme	sequence	1040:1047	arg1	details					1070:1076	sequence and linkage isomeric details	1040:1076	details	1070:1076	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	6	87	from	20	1285:1286	arg1	moojeni					1327:1333	moojeni	1327:1333	moojeni	1327:1333	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	6	87	from	20	1285:1286	arg1	B.					1308:1309	B.	1308:1309	B.	1308:1309	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	6	87	from	20	1285:1286	arg1	B.					1324:1325	B.	1324:1325	B.	1324:1325	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	6	87	from	20	1285:1286	arg1	B.					1339:1340	B.	1339:1340	B.	1339:1340	These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom.					
29716988	5	88	theme	linkage	1053:1059	arg1	details					1070:1076	sequence and linkage isomeric details	1040:1076	details	1070:1076	Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms.					
29716988	3	89	contain	contain	636:642	arg1	glycoproteomes					621:634	Bothrops venom glycoproteomes	606:634	Bothrops venom glycoproteomes	606:634	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	3	89	contain	contain	636:642	arg2	core					646:649	a core	644:649	a core of components that markedly define their composition and parallel their phylogenetic classification	644:749	We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification.					
29716988	1	90	theme	proteins	231:238	arg1	myriad					201:206	a myriad	199:206	a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation	199:373	The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed on envenomation.					
31732167	5	0	theme	B	785:785	arg1	cells					787:791	B cells	785:791	B cells	785:791	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	4	1	theme	B	703:703	arg1	cells					705:709	B cells	703:709	B cells specific for cross-conserved sites	703:744	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	4	2	theme	specific	711:718	arg1	cells					705:709	B cells	703:709	B cells specific for cross-conserved sites	703:744	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	5	3	theme	native-like	849:859	arg1	state					861:865	the native-like state	845:865	the native-like state of the cleavage-independent NFL trimers	845:905	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	1	4	theme	antibodies	183:192	arg1	elicitation					147:157	The elicitation	143:157	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer	143:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	7	5	theme	proof-of-principle	1258:1275	arg1	immunogenicity					1277:1290	proof-of-principle immunogenicity	1258:1290	proof-of-principle immunogenicity toward eliciting bNAbs by vaccination	1258:1328	This study provides proof-of-principle immunogenicity toward eliciting bNAbs by vaccination.					
31732167	5	6	theme	gradual	920:926	arg1	restoration					937:947	gradual N-glycan restoration	920:947	gradual N-glycan restoration coupled with heterologous boosting	920:982	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	2	7	theme	B	382:382	arg1	recognition					389:399	B cell recognition	382:399	B cell recognition of the underlying polypeptide surface	382:437	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	1	8	theme	major	265:269	arg1	challenge					279:287	a major vaccine challenge	263:287	a major vaccine challenge	263:287	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	3	9	dep	glycan	473:478	arg1	shield					480:485	shield	480:485	shield	480:485	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	2	10	theme	protein	311:317	arg1	determinants					319:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	1	11	theme	vaccine	271:277	arg1	challenge					279:287	a major vaccine challenge	263:287	a major vaccine challenge	263:287	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	0	12	theme	Multiple	110:117	arg1	Sites					119:123	Multiple Sites	110:123	Multiple Sites of Vulnerability	110:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	0	12	theme	Multiple	110:117	arg1	Vulnerability					128:140	Vulnerability	128:140	Vulnerability	128:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	4	13	theme	heterologous	639:650	arg1	boosting					674:681	heterologous trimer-liposome prime:boosting	639:681	heterologous trimer-liposome prime:boosting in rabbits	639:692	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	2	14	theme	cross-conserved	295:309	arg1	determinants					319:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	2	15	theme	cell	384:387	arg1	recognition					389:399	B cell recognition	382:399	B cell recognition of the underlying polypeptide surface	382:437	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	3	16	theme	binding	522:528	arg1	site					530:533	receptor CD4 binding site	509:533	receptor CD4 binding site (CD4bs)	509:541	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	3	16	theme	binding	522:528	arg1	CD4bs					536:540	CD4bs	536:540	CD4bs	536:540	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	0	17	theme	Vulnerability	128:140	arg1	Sites					119:123	Multiple Sites	110:123	Multiple Sites of Vulnerability	110:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	0	17	theme	Vulnerability	128:140	arg1	Vulnerability					128:140	Vulnerability	128:140	Vulnerability	128:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	4	18	theme	cross-conserved	724:738	arg1	sites					740:744	cross-conserved sites	724:744	cross-conserved sites	724:744	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	5	19	dep	eliminated	797:806	arg1	followed					908:915	followed	908:915	followed by gradual N-glycan restoration coupled with heterologous boosting	908:982	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	3	20	theme	receptor	509:516	arg1	site					530:533	receptor CD4 binding site	509:533	receptor CD4 binding site (CD4bs)	509:541	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	3	20	theme	receptor	509:516	arg1	CD4bs					536:540	CD4bs	536:540	CD4bs	536:540	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	2	21	theme	Most	290:293	arg1	determinants					319:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants	290:330	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	5	22	theme	trimers	899:905	arg1	state					861:865	the native-like state	845:865	the native-like state of the cleavage-independent NFL trimers	845:905	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	2	23	theme	surface	431:437	arg1	recognition					389:399	B cell recognition	382:399	B cell recognition of the underlying polypeptide surface	382:437	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	5	24	theme	N-glycan	928:935	arg1	restoration					937:947	gradual N-glycan restoration	920:947	gradual N-glycan restoration coupled with heterologous boosting	920:982	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	3	25	theme	gp	561:562	arg1	elements					567:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements	495:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site	495:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	2	26	theme	polypeptide	419:429	arg1	surface					431:437	the underlying polypeptide surface	404:437	the underlying polypeptide surface	404:437	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	3	27	theme	glycoprotein	547:558	arg1	gp					561:562	glycoprotein (gp)41	547:565	glycoprotein (gp)41	547:565	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	4	28	from	boosting	674:681	arg1	rabbits					686:692	rabbits	686:692	rabbits	686:692	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	2	29	theme	underlying	408:417	arg1	surface					431:437	the underlying polypeptide surface	404:437	the underlying polypeptide surface	404:437	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	1	30	gly	glycoprotein	229:240	arg1	Env					243:245	Env	243:245	Env	243:245	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	1	30	gly	glycoprotein	229:240	arg1	glycoprotein					229:240	HIV-1 envelope glycoprotein	214:240	the HIV-1 envelope glycoprotein (Env) trimer	210:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	5	31	theme	cleavage-independent	874:893	arg1	trimers					899:905	the cleavage-independent NFL trimers	870:905	the cleavage-independent NFL trimers	870:905	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	6	32	dep	bNAb	1123:1126	arg1	directed					1142:1149	directed	1142:1149	directed to the gp120:gp41 interface	1142:1177	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	3	33	theme	contiguous	462:471	arg1	glycan					473:478	the contiguous glycan shield	458:485	the contiguous glycan shield	458:485	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	0	34	theme	HIV	33:35	arg1	Trimer-Liposomes					50:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	6	35	theme	CD4bs-directed	1021:1034	arg1	Abs					1056:1058	CD4bs-directed, cross-neutralizing Abs	1021:1058	CD4bs-directed, cross-neutralizing Abs	1021:1058	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	6	35	theme	CD4bs-directed	1021:1034	arg1	bNAb					1123:1126	a bNAb	1121:1126	a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy	1121:1235	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	6	35	theme	CD4bs-directed	1021:1034	arg1	one					1071:1073	one	1071:1073	one targeting a unique glycan-protein epitope	1071:1115	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	6	36	dep	directed	1142:1149	arg1	both					1180:1183	both	1180:1183	both	1180:1183	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	5	37	theme	heterologous	962:973	arg1	boosting					975:982	heterologous boosting	962:982	heterologous boosting	962:982	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	1	38	theme	HIV-1	214:218	arg1	Env					243:245	Env	243:245	Env	243:245	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	1	38	theme	HIV-1	214:218	arg1	glycoprotein					229:240	HIV-1 envelope glycoprotein	214:240	the HIV-1 envelope glycoprotein (Env) trimer	210:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	0	39	theme	Glycan-Modified	17:31	arg1	Trimer-Liposomes					50:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	6	40	theme	high-resolution	1197:1211	arg1	microscopy					1226:1235	high-resolution cryoelectron microscopy	1197:1235	high-resolution cryoelectron microscopy	1197:1235	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	3	41	theme	proximal	576:583	arg1	elements					567:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements	495:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site	495:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	1	42	theme	envelope	220:227	arg1	Env					243:245	Env	243:245	Env	243:245	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	1	42	theme	envelope	220:227	arg1	glycoprotein					229:240	HIV-1 envelope glycoprotein	214:240	the HIV-1 envelope glycoprotein (Env) trimer	210:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	0	43	theme	Envelope	41:48	arg1	Trimer-Liposomes					50:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	1	44	theme	glycoprotein	229:240	arg1	trimer					248:253	the HIV-1 envelope glycoprotein (Env) trimer	210:253	the HIV-1 envelope glycoprotein (Env) trimer	210:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	0	45	theme	NFL	37:39	arg1	Trimer-Liposomes					50:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	4	46	theme	prime	668:672	arg1	boosting					674:681	heterologous trimer-liposome prime:boosting	639:681	heterologous trimer-liposome prime:boosting in rabbits	639:692	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	3	47	theme	furin	592:596	arg1	site					607:610	the furin cleavage site	588:610	the furin cleavage site	588:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	3	48	theme	site	530:533	arg1	elements					567:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements	495:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site	495:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	5	49	theme	proximal	808:815	arg1	N-glycans					817:825	proximal N-glycans	808:825	proximal N-glycans	808:825	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	6	50	theme	cryoelectron	1213:1224	arg1	microscopy					1226:1235	high-resolution cryoelectron microscopy	1197:1235	high-resolution cryoelectron microscopy	1197:1235	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	3	51	theme	cleavage	598:605	arg1	site					607:610	the furin cleavage site	588:610	the furin cleavage site	588:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	0	52	with	Vaccination	0:10	arg1	Trimer-Liposomes					50:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Glycan-Modified HIV NFL Envelope Trimer-Liposomes	17:65	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	3	53	theme	CD4	518:520	arg1	site					530:533	receptor CD4 binding site	509:533	receptor CD4 binding site (CD4bs)	509:541	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	3	53	theme	CD4	518:520	arg1	CD4bs					536:540	CD4bs	536:540	CD4bs	536:540	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	6	54	theme	unique	1087:1092	arg1	epitope					1109:1115	a unique glycan-protein epitope	1085:1115	a unique glycan-protein epitope	1085:1115	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	3	55	gly	glycoprotein	547:558	arg1	glycoprotein					547:558	glycoprotein (gp)41	547:565	glycoprotein (gp)41	547:565	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	2	56	theme	self-N-glycan	348:360	arg1	shielding					362:370	self-N-glycan shielding	348:370	self-N-glycan shielding	348:370	Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface.					
31732167	6	57	theme	gp41	1164:1167	arg1	interface					1169:1177	the gp120:gp41 interface	1154:1177	the gp120:gp41 interface	1154:1177	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	6	58	theme	gp120	1158:1162	arg1	interface					1169:1177	the gp120:gp41 interface	1154:1177	the gp120:gp41 interface	1154:1177	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	0	59	theme	Neutralizing	83:94	arg1	Antibodies					96:105	Broadly Neutralizing Antibodies	75:105	Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability	75:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	6	60	theme	glycan-protein	1094:1107	arg1	epitope					1109:1115	a unique glycan-protein epitope	1085:1115	a unique glycan-protein epitope	1085:1115	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	6	61	dep	CD4bs-directed	1021:1034	arg1	cross-neutralizing					1037:1054	cross-neutralizing	1037:1054	cross-neutralizing	1037:1054	This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy.					
31732167	3	62	theme	conserved	499:507	arg1	elements					567:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements	495:574	the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site	495:610	The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site.					
31732167	1	63	theme	broadly	162:168	arg1	bNAbs					195:199	bNAbs	195:199	bNAbs	195:199	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	1	63	theme	broadly	162:168	arg1	antibodies					183:192	broadly neutralizing antibodies	162:192	broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer	162:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	5	64	theme	NFL	895:897	arg1	trimers					899:905	the cleavage-independent NFL trimers	870:905	the cleavage-independent NFL trimers	870:905	To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting.					
31732167	0	65	theme	Broadly	75:81	arg1	Antibodies					96:105	Broadly Neutralizing Antibodies	75:105	Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability	75:140	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.					
31732167	4	66	theme	trimer-liposome	652:666	arg1	boosting					674:681	heterologous trimer-liposome prime:boosting	639:681	heterologous trimer-liposome prime:boosting in rabbits	639:692	Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites.					
31732167	1	67	theme	neutralizing	170:181	arg1	bNAbs					195:199	bNAbs	195:199	bNAbs	195:199	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31732167	1	67	theme	neutralizing	170:181	arg1	antibodies					183:192	broadly neutralizing antibodies	162:192	broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer	162:253	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge.					
31262188	5	0	gly	glycoproteins	798:810	arg1	glycoproteins					798:810	human salivary glycoproteins	783:810	human salivary glycoproteins	783:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	2	1	theme	blood	337:341	arg1	structures					370:379	blood group antigen carbohydrate structures	337:379	blood group antigen carbohydrate structures	337:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	2	dep	L.	1254:1255	arg1	Day					1110:1112	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human	1051:1261	Day	1110:1112	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	6	2	dep	L.	1254:1255	arg1	C.					1115:1116	C.	1115:1116	C.	1115:1116	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	4	3	theme	galactose	606:614	arg1	Removal					570:576	Removal	570:576	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid	570:654	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	2	4	theme	GAS	261:263	arg1	clone					265:269	the highly virulent M1T1 GAS clone	236:269	the highly virulent M1T1 GAS clone	236:269	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	4	5	theme	N-acetylgalactosamine	617:637	arg1	Removal					570:576	Removal	570:576	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid	570:654	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	6	6	theme	expression	1270:1279	arg1	patterns					1281:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	2	7	theme	oral	283:286	arg1	cells					299:303	oral epithelial cells	283:303	oral epithelial cells	283:303	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	4	8	theme	fucose	598:603	arg1	Removal					570:576	Removal	570:576	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid	570:654	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	5	9	theme	human	783:787	arg1	glycoproteins					798:810	human salivary glycoproteins	783:810	human salivary glycoproteins	783:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	4	10	theme	GAS	666:668	arg1	colonization					670:681	GAS colonization	666:681	GAS colonization	666:681	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	3	11	theme	Lewis	539:543	arg1	expression					558:567	Lewis (Le) antigen expression	539:567	Lewis (Le) antigen expression	539:567	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	5	12	theme	potent	846:851	arg1	inhibitors					853:862	potent inhibitors	846:862	potent inhibitors of M1, M3, and M12 GAS colonization ex vivo	846:906	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	2	13	theme	virulent	247:254	arg1	clone					265:269	the highly virulent M1T1 GAS clone	236:269	the highly virulent M1T1 GAS clone	236:269	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	14	theme	epithelial	1338:1347	arg1	cells					1349:1353	epithelial cells	1338:1353	epithelial cells	1338:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	1	15	theme	initial	137:143	arg1	Colonization					99:110	Colonization	99:110	Colonization of the oropharynx	99:128	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	1	15	theme	initial	137:143	arg1	step					145:148	the initial step	133:148	the initial step in Group A Streptococcus (GAS) pharyngeal infection	133:200	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	3	16	theme	antigen	550:556	arg1	expression					558:567	Lewis (Le) antigen expression	539:567	Lewis (Le) antigen expression	539:567	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	4	17	theme	acid	651:654	arg1	Removal					570:576	Removal	570:576	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid	570:654	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	0	18	theme	epithelial	81:90	arg1	cells					92:96	epithelial cells	81:96	epithelial cells	81:96	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	1	19	theme	A	159:159	arg1	Streptococcus					161:173	Group A Streptococcus	153:173	Group A Streptococcus	153:173	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	4	20	theme	H	706:706	arg1	group					715:719	host ABO(H) blood group and Le expression profile	697:745	group	715:719	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	1	21	from	step	145:148	arg1	infection					192:200	Group A Streptococcus (GAS) pharyngeal infection	153:200	Group A Streptococcus (GAS) pharyngeal infection	153:200	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	5	22	theme	M12	879:881	arg1	colonization					887:898	M12 GAS colonization	879:898	M12 GAS colonization	879:898	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	4	23	theme	blood	709:713	arg1	group					715:719	host ABO(H) blood group and Le expression profile	697:745	group	715:719	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	3	24	theme	human	428:432	arg1	cells					450:454	human oral epithelial cells	428:454	human oral epithelial cells	428:454	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	5	25	theme	colonization	887:898	arg1	inhibitors					853:862	potent inhibitors	846:862	potent inhibitors of M1, M3, and M12 GAS colonization ex vivo	846:906	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	0	26	theme	Human	0:4	arg1	patterns					24:31	Human glycan expression patterns	0:31	Human glycan expression patterns	0:31	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	6	27	theme	protein	965:971	arg1	patterns					987:994	human protein glycosylation patterns	959:994	human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	959:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	4	28	theme	ABO	702:704	arg1	group					715:719	host ABO(H) blood group and Le expression profile	697:745	group	715:719	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	0	29	theme	expression	13:22	arg1	patterns					24:31	Human glycan expression patterns	0:31	Human glycan expression patterns	0:31	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	3	30	theme	epithelial	439:448	arg1	cells					450:454	human oral epithelial cells	428:454	human oral epithelial cells	428:454	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	0	31	theme	A	49:49	arg1	streptococcal					51:63	Group A streptococcal	43:63	Group A streptococcal colonization of epithelial cells	43:96	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	2	32	theme	protein	312:318	arg1	interaction					320:330	M1 protein interaction	309:330	M1 protein interaction with blood group antigen carbohydrate structures	309:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	3	33	theme	group	511:515	arg1	antigens					517:524	human blood group antigens [ABO(H)]	499:533	human blood group antigens [ABO(H)]	499:533	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	6	34	theme	epithelial	1022:1031	arg1	surfaces.-De					1038:1049	oral epithelial cell surfaces.-De	1017:1049	oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	1017:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	4	35	theme	expression	728:737	arg1	profile					739:745	host ABO(H) blood group and Le expression profile	697:745	profile	739:745	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	2	36	theme	carbohydrate	357:368	arg1	structures					370:379	blood group antigen carbohydrate structures	337:379	blood group antigen carbohydrate structures	337:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	2	37	theme	group	343:347	arg1	structures					370:379	blood group antigen carbohydrate structures	337:379	blood group antigen carbohydrate structures	337:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	38	theme	GAS	999:1001	arg1	attachment					1003:1012	GAS attachment	999:1012	GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	999:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	6	39	theme	streptococcal	1308:1320	arg1	colonization					1322:1333	A streptococcal colonization	1306:1333	A streptococcal colonization of epithelial cells	1306:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	6	40	from	patterns	987:994	arg1	attachment					1003:1012	GAS attachment	999:1012	GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	999:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	3	41	theme	blood	505:509	arg1	antigens					517:524	human blood group antigens [ABO(H)]	499:533	human blood group antigens [ABO(H)]	499:533	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	5	42	theme	salivary	789:796	arg1	glycoproteins					798:810	human salivary glycoproteins	783:810	human salivary glycoproteins	783:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	2	43	theme	epithelial	288:297	arg1	cells					299:303	oral epithelial cells	283:303	oral epithelial cells	283:303	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	44	theme	glycan	1263:1268	arg1	patterns					1281:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	1	45	theme	pharyngeal	181:190	arg1	infection					192:200	Group A Streptococcus (GAS) pharyngeal infection	153:200	Group A Streptococcus (GAS) pharyngeal infection	153:200	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	6	46	theme	L.	1254:1255	arg1	patterns					1281:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns	1051:1288	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	2	47	attach	attaches	271:278	arg1	cells					299:303	oral epithelial cells	283:303	oral epithelial cells	283:303	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	2	47	attach	attaches	271:278	arg2	clone					265:269	the highly virulent M1T1 GAS clone	236:269	the highly virulent M1T1 GAS clone	236:269	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	4	48	theme	linkage-specific	581:596	arg1	fucose					598:603	linkage-specific fucose	581:603	linkage-specific fucose	581:603	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	1	49	theme	oropharynx	119:128	arg1	Colonization					99:110	Colonization	99:110	Colonization of the oropharynx	99:128	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	1	49	theme	oropharynx	119:128	arg1	step					145:148	the initial step	133:148	the initial step in Group A Streptococcus (GAS) pharyngeal infection	133:200	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	2	50	theme	M1T1	256:259	arg1	clone					265:269	the highly virulent M1T1 GAS clone	236:269	the highly virulent M1T1 GAS clone	236:269	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	51	theme	cells	1349:1353	arg1	colonization					1322:1333	A streptococcal colonization	1306:1333	A streptococcal colonization of epithelial cells	1306:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	3	52	dep	antigens	517:524	arg1	H					531:531	H	531:531	H	531:531	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	3	52	dep	antigens	517:524	arg1	[ABO					526:529	[ABO	526:529	human blood group antigens [ABO(H)]	499:533	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	6	53	theme	important	934:942	arg1	role					944:947	the important role	930:947	the important role	930:947	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	5	54	theme	N-linked	761:768	arg1	glycans					770:776	N-linked glycans	761:776	N-linked glycans from human salivary glycoproteins	761:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	0	55	theme	cells	92:96	arg1	colonization					65:76	Group A streptococcal colonization	43:76	Group A streptococcal colonization of epithelial cells	43:96	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	5	56	from	glycoproteins	798:810	arg1	glycans					770:776	N-linked glycans	761:776	N-linked glycans from human salivary glycoproteins	761:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	1	57	theme	Group	153:157	arg1	Streptococcus					161:173	Group A Streptococcus	153:173	Group A Streptococcus	153:173	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	4	58	theme	sialic	644:649	arg1	acid					651:654	sialic acid	644:654	sialic acid	644:654	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	5	59	link	N-linked	761:768	arg1	glycans					770:776	N-linked glycans	761:776	N-linked glycans from human salivary glycoproteins	761:810	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	3	60	dep	serotypes	463:471	arg1	serotypes					463:471	GAS serotypes M3 and M12	459:482	GAS serotypes M3 and M12	459:482	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	3	60	dep	serotypes	463:471	arg1	M12					480:482	M12	480:482	M12	480:482	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	3	60	dep	serotypes	463:471	arg1	M3					473:474	M3	473:474	M3	473:474	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	6	61	dep	highlight	920:928	arg1	played					949:954	played	949:954	highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	920:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	5	62	theme	GAS	883:885	arg1	colonization					887:898	M12 GAS colonization	879:898	M12 GAS colonization	879:898	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	3	63	theme	oral	434:437	arg1	cells					450:454	human oral epithelial cells	428:454	human oral epithelial cells	428:454	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	0	64	theme	glycan	6:11	arg1	patterns					24:31	Human glycan expression patterns	0:31	Human glycan expression patterns	0:31	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	5	65	theme	M1	867:868	arg1	inhibitors					853:862	potent inhibitors	846:862	potent inhibitors of M1, M3, and M12 GAS colonization ex vivo	846:906	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	6	66	theme	glycosylation	973:985	arg1	patterns					987:994	human protein glycosylation patterns	959:994	human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	959:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	4	67	theme	dependent	684:692	arg1	colonization					670:681	GAS colonization	666:681	GAS colonization	666:681	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	1	68	dep	pharyngeal	181:190	arg1	Streptococcus					161:173	Group A Streptococcus	153:173	Group A Streptococcus	153:173	Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection.					
31262188	5	69	theme	M3	871:872	arg1	inhibitors					853:862	potent inhibitors	846:862	potent inhibitors of M1, M3, and M12 GAS colonization ex vivo	846:906	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	6	70	theme	human	959:963	arg1	patterns					987:994	human protein glycosylation patterns	959:994	human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	959:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	4	71	theme	host	697:700	arg1	group					715:719	host ABO(H) blood group and Le expression profile	697:745	group	715:719	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	3	72	theme	GAS	459:461	arg1	serotypes					463:471	GAS serotypes M3 and M12	459:482	GAS serotypes M3 and M12	459:482	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	3	72	theme	GAS	459:461	arg1	M12					480:482	M12	480:482	M12	480:482	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	3	72	theme	GAS	459:461	arg1	M3					473:474	M3	473:474	M3	473:474	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	0	73	theme	Group	43:47	arg1	streptococcal					51:63	Group A streptococcal	43:63	Group A streptococcal colonization of epithelial cells	43:96	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	5	74	dep	ex	900:901	arg1	vivo					903:906	vivo	903:906	vivo	903:906	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	3	75	theme	cells	450:454	arg1	colonization					412:423	colonization	412:423	colonization of human oral epithelial cells by GAS serotypes M3 and M12	412:482	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
31262188	0	76	theme	streptococcal	51:63	arg1	colonization					65:76	Group A streptococcal colonization	43:76	Group A streptococcal colonization of epithelial cells	43:96	Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	2	77	with	interaction	320:330	arg1	structures					370:379	blood group antigen carbohydrate structures	337:379	blood group antigen carbohydrate structures	337:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	2	78	theme	antigen	349:355	arg1	structures					370:379	blood group antigen carbohydrate structures	337:379	blood group antigen carbohydrate structures	337:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	4	79	theme	Le	725:726	arg1	profile					739:745	host ABO(H) blood group and Le expression profile	697:745	profile	739:745	Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile.					
31262188	5	80	theme	ex	900:901	arg1	M1					867:868	M1	867:868	M1	867:868	Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo.					
31262188	6	81	theme	cell	1033:1036	arg1	surfaces.-De					1038:1049	oral epithelial cell surfaces.-De	1017:1049	oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	1017:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	2	82	theme	M1	309:310	arg1	interaction					320:330	M1 protein interaction	309:330	M1 protein interaction with blood group antigen carbohydrate structures	309:379	We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures.					
31262188	6	83	theme	oral	1017:1020	arg1	surfaces.-De					1038:1049	oral epithelial cell surfaces.-De	1017:1049	oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells	1017:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	6	84	dep	surfaces.-De	1038:1049	arg1	influence					1290:1298	influence	1290:1298	influence Group A streptococcal colonization of epithelial cells	1290:1353	These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.					
31262188	3	85	theme	human	499:503	arg1	antigens					517:524	human blood group antigens [ABO(H)]	499:533	human blood group antigens [ABO(H)]	499:533	Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression.					
30017654	0	0	theme	Chinese	98:104	arg1	cells					120:124	Chinese Hamster Ovary cells	98:124	Chinese Hamster Ovary cells	98:124	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	8	1	theme	N-glycan	1362:1369	arg1	maturation					1371:1380	N-glycan maturation	1362:1380	N-glycan maturation	1362:1380	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	9	2	from	targets	1616:1622	arg1	context					1467:1473	the context	1463:1473	the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation	1463:1576	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	5	3	theme	processing	953:962	arg1	status					964:969	their N-glycan processing status	938:969	their N-glycan processing status	938:969	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	0	4	from	contributions	4:16	arg1	cells					120:124	Chinese Hamster Ovary cells	98:124	Chinese Hamster Ovary cells	98:124	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	9	5	theme	productivity	1483:1494	arg1	lines					1501:1505	high productivity cell lines	1478:1505	high productivity cell lines	1478:1505	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	1	6	theme	dominant	262:269	arg1	isoenzyme					271:279	the dominant isoenzyme	258:279	the dominant isoenzyme for this reaction	258:297	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	0	7	theme	Ovary	114:118	arg1	cells					120:124	Chinese Hamster Ovary cells	98:124	Chinese Hamster Ovary cells	98:124	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	8	8	theme	oligosaccharides	1442:1457	arg1	occurrence					1411:1420	a higher occurrence	1402:1420	a higher occurrence of poorly processed oligosaccharides	1402:1457	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	4	9	theme	structures	822:831	arg1	spectrum					792:799	a large spectrum	784:799	a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase	784:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	3	10	theme	site-specific	533:545	arg1	activities					547:556	the protein- and site-specific activities	516:556	the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells	516:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	3	11	from	activities	547:556	arg1	cells					620:624	Chinese Hamster Ovary cells	598:624	Chinese Hamster Ovary cells	598:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	0	12	theme	Hamster	106:112	arg1	cells					120:124	Chinese Hamster Ovary cells	98:124	Chinese Hamster Ovary cells	98:124	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	9	13	theme	product	1641:1647	arg1	quality					1649:1655	stable product quality	1634:1655	stable product quality	1634:1655	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	8	14	theme	maturation	1371:1380	arg1	process					1351:1357	the overall process	1339:1357	the overall process of N-glycan maturation	1339:1380	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	10	15	theme	engineered	1695:1704	arg1	lines					1722:1726	specifically engineered "designer" cell lines	1682:1726	specifically engineered "designer" cell lines	1682:1726	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	3	16	theme	activities	547:556	arg1	understanding					499:511	the understanding	495:511	the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells	495:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	10	17	theme	different	1748:1756	arg1	proteins					1758:1765	different proteins	1748:1765	different proteins	1748:1765	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	7	18	theme	model	1264:1268	arg1	proteins					1270:1277	the two model proteins	1256:1277	the two model proteins	1256:1277	Interestingly, the contributions of B4GALT2 and B4GALT3 differed for the two model proteins.					
30017654	9	19	theme	lines	1501:1505	arg1	context					1467:1473	the context	1463:1473	the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation	1463:1576	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	3	20	theme	protein-	520:527	arg1	activities					547:556	the protein- and site-specific activities	516:556	the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells	516:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	9	21	theme	high	1478:1481	arg1	lines					1501:1505	high productivity cell lines	1478:1505	high productivity cell lines	1478:1505	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	6	22	theme	%	1081:1081	arg1	decrease					1063:1070	a decrease	1061:1070	a decrease of 15-21 % of fully galactosylated structures for erythropoietin	1061:1135	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	9	23	theme	cell	1496:1499	arg1	lines					1501:1505	high productivity cell lines	1478:1505	high productivity cell lines	1478:1505	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	3	24	theme	B4GALT	714:719	arg1	isoform					703:709	only one isoform	694:709	only one isoform of B4GALT each	694:724	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	6	25	theme	isoenzymes	1175:1184	arg1	involvement					1154:1164	the involvement	1150:1164	the involvement of other isoenzymes	1150:1184	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	6	26	theme	other	1169:1173	arg1	isoenzymes					1175:1184	other isoenzymes	1169:1184	other isoenzymes	1169:1184	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	7	27	theme	B4GALT3	1235:1241	arg1	contributions					1206:1218	the contributions	1202:1218	the contributions of B4GALT2 and B4GALT3	1202:1241	Interestingly, the contributions of B4GALT2 and B4GALT3 differed for the two model proteins.					
30017654	3	28	theme	galactosyltransferases	572:593	arg1	activities					547:556	the protein- and site-specific activities	516:556	the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells	516:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	2	29	theme	isoenzymes	381:390	arg1	B4GALT4					442:448	B4GALT4	442:448	B4GALT4	442:448	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	29	theme	isoenzymes	381:390	arg1	B4GALT3					430:436	B4GALT3	430:436	B4GALT3	430:436	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	29	theme	isoenzymes	381:390	arg1	B4GALT2					421:427	B4GALT2	421:427	B4GALT2	421:427	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	29	theme	isoenzymes	381:390	arg1	contributions					324:336	the exact contributions	314:336	the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation	314:418	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	5	30	dep	constructs	903:912	arg1	constructs					903:912	Fc-fusion constructs	893:912	Fc-fusion constructs (EPO-Fc and Fc-DAO)	893:932	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	5	30	dep	constructs	903:912	arg1	Fc-DAO					926:931	Fc-DAO	926:931	Fc-DAO	926:931	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	5	30	dep	constructs	903:912	arg1	EPO-Fc					915:920	EPO-Fc	915:920	EPO-Fc	915:920	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	9	31	theme	stable	1634:1639	arg1	quality					1649:1655	stable product quality	1634:1655	stable product quality	1634:1655	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	2	32	theme	exact	318:322	arg1	B4GALT4					442:448	B4GALT4	442:448	B4GALT4	442:448	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	32	theme	exact	318:322	arg1	B4GALT3					430:436	B4GALT3	430:436	B4GALT3	430:436	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	32	theme	exact	318:322	arg1	B4GALT2					421:427	B4GALT2	421:427	B4GALT2	421:427	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	32	theme	exact	318:322	arg1	contributions					324:336	the exact contributions	314:336	the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation	314:418	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	33	theme	other	375:379	arg1	isoenzymes					381:390	the other isoenzymes	371:390	the other isoenzymes involved in N-glycosylation	371:418	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	0	34	theme	individual	21:30	arg1	galactosyltransferases					32:53	individual galactosyltransferases	21:53	individual galactosyltransferases	21:53	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	10	35	theme	designer	1707:1714	arg1	lines					1722:1726	specifically engineered "designer" cell lines	1682:1726	specifically engineered "designer" cell lines	1682:1726	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	4	36	theme	N-glycan	813:820	arg1	deamine-oxidase					853:867	deamine-oxidase	853:867	deamine-oxidase	853:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	36	theme	N-glycan	813:820	arg1	erythropoietin					834:847	erythropoietin	834:847	erythropoietin	834:847	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	36	theme	N-glycan	813:820	arg1	structures					822:831	possible N-glycan structures	804:831	possible N-glycan structures: erythropoietin and deamine-oxidase	804:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	5	37	theme	N-glycan	944:951	arg1	status					964:969	their N-glycan processing status	938:969	their N-glycan processing status	938:969	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	4	38	theme	possible	804:811	arg1	deamine-oxidase					853:867	deamine-oxidase	853:867	deamine-oxidase	853:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	38	theme	possible	804:811	arg1	erythropoietin					834:847	erythropoietin	834:847	erythropoietin	834:847	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	38	theme	possible	804:811	arg1	structures					822:831	possible N-glycan structures	804:831	possible N-glycan structures: erythropoietin and deamine-oxidase	804:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	1	39	theme	beta-1,4-galactosyltransferases	199:229	arg1	set					192:194	a set	190:194	a set of beta-1,4-galactosyltransferases (B4GALTs)	190:239	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	1	39	theme	beta-1,4-galactosyltransferases	199:229	arg1	B4GALTs					232:238	B4GALTs	232:238	B4GALTs	232:238	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	1	39	theme	beta-1,4-galactosyltransferases	199:229	arg1	beta-1,4-galactosyltransferases					199:229	beta-1,4-galactosyltransferases	199:229	beta-1,4-galactosyltransferases (B4GALTs)	199:239	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	4	40	theme	model	731:735	arg1	proteins					737:744	Two model proteins	727:744	Two model proteins	727:744	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	9	41	theme	potential	1606:1614	arg1	galactosyltransferases					1579:1600	galactosyltransferases	1579:1600	galactosyltransferases	1579:1600	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	9	41	theme	potential	1606:1614	arg1	targets					1616:1622	potential targets	1606:1622	potential targets	1606:1622	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	3	42	theme	cell	654:657	arg1	lines					659:663	triple deletion cell lines	638:663	triple deletion cell lines	638:663	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	3	43	theme	individual	561:570	arg1	galactosyltransferases					572:593	individual galactosyltransferases	561:593	individual galactosyltransferases in Chinese Hamster Ovary cells	561:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	6	44	theme	structures	1107:1116	arg1	structures					1107:1116	fully galactosylated structures	1086:1116	fully galactosylated structures for erythropoietin	1086:1135	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	6	44	theme	structures	1107:1116	arg1	%					1081:1081	15-21 %	1075:1081	15-21 % of fully galactosylated structures for erythropoietin	1075:1135	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	3	45	theme	lines	659:663	arg1	panel					629:633	a panel	627:633	a panel of triple deletion cell lines	627:663	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	3	46	theme	Ovary	614:618	arg1	cells					620:624	Chinese Hamster Ovary cells	598:624	Chinese Hamster Ovary cells	598:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	3	47	from	cells	620:624	arg1	activities					547:556	the protein- and site-specific activities	516:556	the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells	516:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	10	48	theme	cell	1717:1720	arg1	lines					1722:1726	specifically engineered "designer" cell lines	1682:1726	specifically engineered "designer" cell lines	1682:1726	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	0	49	theme	galactosyltransferases	32:53	arg1	contributions					4:16	The contributions	0:16	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells	0:124	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	3	50	dep	B4GALT	714:719	arg1	each					721:724	each	721:724	each	721:724	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	10	51	theme	results	1673:1679	arg1	view					1661:1664	view	1661:1664	view of our results	1661:1679	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	3	52	theme	triple	638:643	arg1	lines					659:663	triple deletion cell lines	638:663	triple deletion cell lines	638:663	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	8	53	theme	occurrence	1411:1420	arg1	result					1392:1397	the result	1388:1397	the result of a higher occurrence of poorly processed oligosaccharides	1388:1457	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	8	54	theme	overall	1343:1349	arg1	process					1351:1357	the overall process	1339:1357	the overall process of N-glycan maturation	1339:1380	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	6	55	theme	B4GALT1	1041:1047	arg1	activity					1029:1036	The sole activity	1020:1036	The sole activity of B4GALT1	1020:1047	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	0	56	theme	specific	66:73	arg1	processing					84:93	protein specific N-glycan processing	58:93	protein specific N-glycan processing	58:93	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	3	57	theme	deletion	645:652	arg1	lines					659:663	triple deletion cell lines	638:663	triple deletion cell lines	638:663	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	3	58	theme	Chinese	598:604	arg1	cells					620:624	Chinese Hamster Ovary cells	598:624	Chinese Hamster Ovary cells	598:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	8	59	theme	galactosyltransferases	1305:1326	arg1	removal					1294:1300	removal	1294:1300	removal of galactosyltransferases	1294:1326	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	0	60	theme	protein	58:64	arg1	processing					84:93	protein specific N-glycan processing	58:93	protein specific N-glycan processing	58:93	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	8	61	theme	processed	1432:1440	arg1	oligosaccharides					1442:1457	poorly processed oligosaccharides	1425:1457	poorly processed oligosaccharides	1425:1457	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	3	62	theme	Hamster	606:612	arg1	cells					620:624	Chinese Hamster Ovary cells	598:624	Chinese Hamster Ovary cells	598:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	2	63	theme	key-player	346:355	arg1	B4GALT4					442:448	B4GALT4	442:448	B4GALT4	442:448	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	63	theme	key-player	346:355	arg1	B4GALT3					430:436	B4GALT3	430:436	B4GALT3	430:436	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	63	theme	key-player	346:355	arg1	B4GALT2					421:427	B4GALT2	421:427	B4GALT2	421:427	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	2	63	theme	key-player	346:355	arg1	contributions					324:336	the exact contributions	314:336	the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation	314:418	Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.					
30017654	5	64	theme	site-specific	987:999	arg1	spectrometry					1006:1017	site-specific mass spectrometry	987:1017	site-specific mass spectrometry	987:1017	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	9	65	theme	incomplete	1551:1560	arg1	galactosylation					1562:1576	incomplete galactosylation	1551:1576	incomplete galactosylation	1551:1576	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	9	66	theme	N-glycan	1523:1530	arg1	maturation					1532:1541	N-glycan maturation	1523:1541	N-glycan maturation	1523:1541	In the context of high productivity cell lines, which can push N-glycan maturation towards incomplete galactosylation, galactosyltransferases are potential targets to ensure stable product quality.					
30017654	8	67	theme	higher	1404:1409	arg1	occurrence					1411:1420	a higher occurrence	1402:1420	a higher occurrence of poorly processed oligosaccharides	1402:1457	Unexpectedly, removal of galactosyltransferases influenced the overall process of N-glycan maturation, with the result of a higher occurrence of poorly processed oligosaccharides.					
30017654	5	68	theme	mass	1001:1004	arg1	spectrometry					1006:1017	site-specific mass spectrometry	987:1017	site-specific mass spectrometry	987:1017	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	10	69	theme	"	1715:1715	arg1	lines					1722:1726	specifically engineered "designer" cell lines	1682:1726	specifically engineered "designer" cell lines	1682:1726	In view of our results, specifically engineered "designer" cell lines may be required for different proteins.					
30017654	5	70	theme	Fc-fusion	893:901	arg1	constructs					903:912	Fc-fusion constructs	893:912	Fc-fusion constructs (EPO-Fc and Fc-DAO)	893:932	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	5	70	theme	Fc-fusion	893:901	arg1	They					870:873	They	870:873	They	870:873	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	5	70	theme	Fc-fusion	893:901	arg1	Fc-DAO					926:931	Fc-DAO	926:931	Fc-DAO	926:931	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	5	70	theme	Fc-fusion	893:901	arg1	EPO-Fc					915:920	EPO-Fc	915:920	EPO-Fc	915:920	They were expressed as Fc-fusion constructs (EPO-Fc and Fc-DAO) and their N-glycan processing status was analyzed by site-specific mass spectrometry.					
30017654	3	71	from	galactosyltransferases	572:593	arg1	cells					620:624	Chinese Hamster Ovary cells	598:624	Chinese Hamster Ovary cells	598:624	To increase the understanding of the protein- and site-specific activities of individual galactosyltransferases in Chinese Hamster Ovary cells, a panel of triple deletion cell lines was generated that expressed only one isoform of B4GALT each.					
30017654	6	72	theme	galactosylated	1092:1105	arg1	structures					1107:1116	fully galactosylated structures	1086:1116	fully galactosylated structures for erythropoietin	1086:1135	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	4	73	dep	structures	822:831	arg1	deamine-oxidase					853:867	deamine-oxidase	853:867	deamine-oxidase	853:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	73	dep	structures	822:831	arg1	erythropoietin					834:847	erythropoietin	834:847	erythropoietin	834:847	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	4	73	dep	structures	822:831	arg1	structures					822:831	possible N-glycan structures	804:831	possible N-glycan structures: erythropoietin and deamine-oxidase	804:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	1	74	theme	N-glycan	154:161	arg1	processing					163:172	N-glycan processing	154:172	N-glycan processing	154:172	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	6	75	theme	sole	1024:1027	arg1	activity					1029:1036	The sole activity	1020:1036	The sole activity of B4GALT1	1020:1047	The sole activity of B4GALT1 resulted in a decrease of 15-21 % of fully galactosylated structures for erythropoietin, emphasizing the involvement of other isoenzymes.					
30017654	0	76	theme	N-glycan	75:82	arg1	processing					84:93	protein specific N-glycan processing	58:93	protein specific N-glycan processing	58:93	The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells.					
30017654	4	77	theme	large	786:790	arg1	spectrum					792:799	a large spectrum	784:799	a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase	784:867	Two model proteins were selected for this study to cover a large spectrum of possible N-glycan structures: erythropoietin and deamine-oxidase.					
30017654	1	78	theme	processing	163:172	arg1	part					146:149	part	146:149	part of N-glycan processing	146:172	Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction.					
30017654	7	79	theme	B4GALT2	1223:1229	arg1	contributions					1206:1218	the contributions	1202:1218	the contributions of B4GALT2 and B4GALT3	1202:1241	Interestingly, the contributions of B4GALT2 and B4GALT3 differed for the two model proteins.					
29475940	10	0	theme	core	1699:1702	arg1	fucose					1704:1709	core fucose	1699:1709	core fucose	1699:1709	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	11	1	theme	fucose	1794:1799	arg1	patterns					1815:1822	core fucose glycosylation patterns	1789:1822	core fucose glycosylation patterns in different planarian strains	1789:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	6	2	theme	extensive	983:991	arg1	patterns					1021:1028	extensive, yet selective, methylation patterns	983:1028	extensive, yet selective, methylation patterns	983:1028	N-Glycans showed extensive, yet selective, methylation patterns, ranging from non-methylated to polymethylated glycoforms.					
29475940	10	3	theme	multiple	1732:1739	arg1	isomers					1741:1747	multiple isomers	1732:1747	multiple isomers	1732:1747	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	2	4	theme	GalFuc	324:329	arg1	fucose					316:321	core fucose	311:321	core fucose (GalFuc epitope)	311:338	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	4	theme	GalFuc	324:329	arg1	epitope					331:337	GalFuc epitope	324:337	GalFuc epitope	324:337	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	9	5	theme	S.	1577:1578	arg1	mediterranea					1580:1591	S. mediterranea	1577:1591	S. mediterranea	1577:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	4	6	link	N-linked	596:603	arg1	oligosaccharides					605:620	N-linked oligosaccharides	596:620	N-linked oligosaccharides from the planarian Schmidtea mediterranea	596:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	11	7	theme	different	1827:1835	arg1	strains					1847:1853	different planarian strains	1827:1853	different planarian strains	1827:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	13	8	theme	pathway	2168:2174	arg1	presence					2122:2129	the presence	2118:2129	the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea	2118:2193	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	2	9	theme	fucose	316:321	arg1	galactosylation					292:306	The galactosylation	288:306	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans	288:381	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	9	theme	fucose	316:321	arg1	characteristic					386:399	characteristic	386:399	characteristic of protostome organisms, including flatworms (planarians)	386:457	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	11	10	from	variations	1775:1784	arg1	patterns					1815:1822	core fucose glycosylation patterns	1789:1822	core fucose glycosylation patterns in different planarian strains	1789:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	9	11	from	nematodes	1477:1485	arg1	homologs					1425:1432	two potential homologs	1411:1432	two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1411:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	12	12	theme	various	1920:1926	arg1	modifications					1944:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications and methylations create >60 different glycoforms in S. mediterranea.					
29475940	7	13	theme	non-methylated	1181:1194	arg1	glycans					1196:1202	non-methylated glycans	1181:1202	non-methylated glycans	1181:1202	Although the majority of glycoforms were polymethylated, a small fraction also consisted of non-methylated glycans.					
29475940	13	14	theme	S.	2179:2180	arg1	mediterranea					2182:2193	S. mediterranea	2179:2193	S. mediterranea	2179:2193	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	5	15	theme	dominant	946:953	arg1	glycomers					955:963	the most dominant glycomers	937:963	the most dominant glycomers	937:963	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	15	theme	dominant	946:953	arg1	fucoses					924:930	trigalactosylated core fucoses	901:930	trigalactosylated core fucoses	901:930	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	15	theme	dominant	946:953	arg1	Di-					893:895	Di-	893:895	Di-	893:895	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	12	16	theme	chitobiose	1933:1942	arg1	modifications					1944:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications and methylations create >60 different glycoforms in S. mediterranea.					
29475940	4	17	theme	paucimannose	724:735	arg1	structures					737:746	multiple isomeric high-mannose and paucimannose structures	689:746	structures	737:746	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	5	18	theme	trigalactosylated	901:917	arg1	glycomers					955:963	the most dominant glycomers	937:963	the most dominant glycomers	937:963	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	18	theme	trigalactosylated	901:917	arg1	fucoses					924:930	trigalactosylated core fucoses	901:930	trigalactosylated core fucoses	901:930	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	18	theme	trigalactosylated	901:917	arg1	Di-					893:895	Di-	893:895	Di-	893:895	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	8	19	theme	selective	1349:1357	arg1	methylation					1359:1369	structurally selective methylation	1336:1369	structurally selective methylation	1336:1369	Remarkably, monogalactosylated core fucose remained unmethylated, whereas its polygalactosylated forms were methylated, indicating structurally selective methylation.					
29475940	4	20	theme	=	799:799	arg1	n					797:797	n = 3-5	797:803	n = 3-5	797:803	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	1	21	theme	surface-associated	147:164	arg1	glycans					166:172	Cell surface-associated glycans	142:172	Cell surface-associated glycans	142:172	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	2	22	theme	core	311:314	arg1	fucose					316:321	core fucose	311:321	core fucose (GalFuc epitope)	311:338	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	22	theme	core	311:314	arg1	epitope					331:337	GalFuc epitope	324:337	GalFuc epitope	324:337	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	13	23	attach	presence	2122:2129	arg2	pathway					2168:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	13	23	attach	presence	2122:2129	arg1	mediterranea					2182:2193	S. mediterranea	2179:2193	S. mediterranea	2179:2193	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	13	24	theme	biosynthetic	2155:2166	arg1	pathway					2168:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	0	25	theme	paucimannose	78:89	arg1	N-glycans					91:99	paucimannose N-glycans	78:99	paucimannose N-glycans	78:99	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	1	26	theme	cellular	187:194	arg1	organization					274:285	extracellular matrix organization	253:285	extracellular matrix organization	253:285	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	26	theme	cellular	187:194	arg1	signaling					238:246	signaling	238:246	signaling	238:246	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	26	theme	cellular	187:194	arg1	migration					227:235	migration	227:235	migration	227:235	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	26	theme	cellular	187:194	arg1	processes					196:204	many cellular processes	182:204	many cellular processes	182:204	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	26	theme	cellular	187:194	arg1	adhesion					217:224	adhesion	217:224	adhesion	217:224	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	0	27	from	Identification	0:13	arg1	mediterranea					128:139	the planarian Schmidtea mediterranea	104:139	the planarian Schmidtea mediterranea	104:139	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	4	28	theme	latter	834:839	arg1	structures					841:850	the latter structures	830:850	the latter structures	830:850	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	0	29	theme	planarian	108:116	arg1	mediterranea					128:139	the planarian Schmidtea mediterranea	104:139	the planarian Schmidtea mediterranea	104:139	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	9	30	theme	enzyme	1465:1470	arg1	homologs					1425:1432	two potential homologs	1411:1432	two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1411:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	4	31	dep	polygalactosylated	777:794	arg1	n					797:797	n = 3-5	797:803	n = 3-5	797:803	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	6	32	theme	polymethylated	1062:1075	arg1	glycoforms					1077:1086	polymethylated glycoforms	1062:1086	polymethylated glycoforms	1062:1086	N-Glycans showed extensive, yet selective, methylation patterns, ranging from non-methylated to polymethylated glycoforms.					
29475940	11	33	from	adaptation	1880:1889	arg1	glycosylation					1901:1913	fucose glycosylation	1894:1913	fucose glycosylation	1894:1913	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	10	34	theme	homologs	1621:1628	arg1	presence					1598:1605	The presence	1594:1605	The presence of two GALT-1 homologs	1594:1628	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	4	35	from	mediterranea	651:662	arg1	analysis					584:591	Whole-organism MALDI-MS analysis	560:591	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea	560:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	35	from	mediterranea	651:662	arg1	oligosaccharides					605:620	N-linked oligosaccharides	596:620	N-linked oligosaccharides from the planarian Schmidtea mediterranea	596:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	36	theme	fucose	811:816	arg1	structures					818:827	polygalactosylated (n = 3-5) core fucose structures	777:827	polygalactosylated (n = 3-5) core fucose structures	777:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	13	37	theme	biosynthesis	2093:2104	arg1	understanding					2067:2079	our understanding	2063:2079	our understanding of N-glycan biosynthesis	2063:2104	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	2	38	from	galactosylation	292:306	arg1	paucimannose					343:354	paucimannose	343:354	paucimannose	343:354	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	38	from	galactosylation	292:306	arg1	N-glycans					373:381	complex-type N-glycans	360:381	complex-type N-glycans	360:381	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	0	39	theme	multiple	18:25	arg1	high-mannose					61:72	multiple isomeric core chitobiose-modified high-mannose	18:72	multiple isomeric core chitobiose-modified high-mannose	18:72	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	4	40	theme	planarian	631:639	arg1	mediterranea					651:662	the planarian Schmidtea mediterranea	627:662	the planarian Schmidtea mediterranea	627:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	10	41	theme	different	1639:1647	arg1	requirements					1649:1660	different requirements	1639:1660	different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers	1639:1747	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	0	42	theme	core	36:39	arg1	high-mannose					61:72	multiple isomeric core chitobiose-modified high-mannose	18:72	multiple isomeric core chitobiose-modified high-mannose	18:72	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	9	43	from	regions	1566:1572	arg1	mediterranea					1580:1591	S. mediterranea	1577:1591	S. mediterranea	1577:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	11	44	from	patterns	1815:1822	arg1	strains					1847:1853	different planarian strains	1827:1853	different planarian strains	1827:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	11	45	theme	evolutionary	1867:1878	arg1	adaptation					1880:1889	evolutionary adaptation	1867:1889	evolutionary adaptation in fucose glycosylation	1867:1913	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	4	46	from	analysis	584:591	arg1	mediterranea					651:662	the planarian Schmidtea mediterranea	627:662	the planarian Schmidtea mediterranea	627:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	47	theme	N-linked	596:603	arg1	oligosaccharides					605:620	N-linked oligosaccharides	596:620	N-linked oligosaccharides from the planarian Schmidtea mediterranea	596:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	1	48	theme	extracellular	253:265	arg1	organization					274:285	extracellular matrix organization	253:285	extracellular matrix organization	253:285	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	8	49	theme	monogalactosylated	1217:1234	arg1	fucose					1241:1246	monogalactosylated core fucose	1217:1246	monogalactosylated core fucose	1217:1246	Remarkably, monogalactosylated core fucose remained unmethylated, whereas its polygalactosylated forms were methylated, indicating structurally selective methylation.					
29475940	11	50	theme	core	1789:1792	arg1	patterns					1815:1822	core fucose glycosylation patterns	1789:1822	core fucose glycosylation patterns in different planarian strains	1789:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	6	51	theme	selective	998:1006	arg1	patterns					1021:1028	extensive, yet selective, methylation patterns	983:1028	extensive, yet selective, methylation patterns	983:1028	N-Glycans showed extensive, yet selective, methylation patterns, ranging from non-methylated to polymethylated glycoforms.					
29475940	12	52	theme	S.	2010:2011	arg1	mediterranea					2013:2024	S. mediterranea	2010:2024	S. mediterranea	2010:2024	The various core chitobiose modifications and methylations create >60 different glycoforms in S. mediterranea.					
29475940	4	53	theme	isomeric	698:705	arg1	high-mannose					707:718	multiple isomeric high-mannose and paucimannose structures	689:746	high-mannose	707:718	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	10	54	theme	fucose	1704:1709	arg1	polygalactosylation					1676:1694	polygalactosylation	1676:1694	polygalactosylation	1676:1694	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	10	54	theme	fucose	1704:1709	arg1	mono-					1666:1670	mono-	1666:1670	mono-	1666:1670	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	2	55	theme	protostome	404:413	arg1	organisms					415:423	protostome organisms	404:423	protostome organisms	404:423	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	55	theme	protostome	404:413	arg1	flatworms					436:444	flatworms	436:444	flatworms (planarians)	436:457	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	9	56	theme	mesenchymal	1554:1564	arg1	regions					1566:1572	the prepharyngeal, pharyngeal, and mesenchymal regions	1519:1572	the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1519:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	10	57	theme	isomers	1741:1747	arg1	formation					1719:1727	the formation	1715:1727	the formation of multiple isomers	1715:1747	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	11	58	theme	glycosylation	1801:1813	arg1	patterns					1815:1822	core fucose glycosylation patterns	1789:1822	core fucose glycosylation patterns in different planarian strains	1789:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	8	59	theme	polygalactosylated	1283:1300	arg1	forms					1302:1306	its polygalactosylated forms	1279:1306	its polygalactosylated forms	1279:1306	Remarkably, monogalactosylated core fucose remained unmethylated, whereas its polygalactosylated forms were methylated, indicating structurally selective methylation.					
29475940	12	60	theme	core	1928:1931	arg1	modifications					1944:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications	1916:1956	The various core chitobiose modifications and methylations create >60 different glycoforms in S. mediterranea.					
29475940	4	61	theme	unusual	753:759	arg1	mono-					761:765	unusual mono-	753:765	unusual mono-	753:765	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	11	62	theme	planarian	1837:1845	arg1	strains					1847:1853	different planarian strains	1827:1853	different planarian strains	1827:1853	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	0	63	theme	high-mannose	61:72	arg1	Identification					0:13	Identification	0:13	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.	0:140	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	7	64	theme	small	1148:1152	arg1	fraction					1154:1161	a small fraction	1146:1161	a small fraction	1146:1161	Although the majority of glycoforms were polymethylated, a small fraction also consisted of non-methylated glycans.					
29475940	9	65	theme	database	1378:1385	arg1	searches					1387:1394	database searches	1378:1394	database searches	1378:1394	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	5	66	theme	core	919:922	arg1	glycomers					955:963	the most dominant glycomers	937:963	the most dominant glycomers	937:963	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	66	theme	core	919:922	arg1	fucoses					924:930	trigalactosylated core fucoses	901:930	trigalactosylated core fucoses	901:930	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	5	66	theme	core	919:922	arg1	Di-					893:895	Di-	893:895	Di-	893:895	Di- and trigalactosylated core fucoses were the most dominant glycomers.					
29475940	4	67	theme	structures	737:746	arg1	presence					677:684	the presence	673:684	the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures	673:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	7	68	theme	glycoforms	1114:1123	arg1	majority					1102:1109	the majority	1098:1109	the majority of glycoforms	1098:1123	Although the majority of glycoforms were polymethylated, a small fraction also consisted of non-methylated glycans.					
29475940	1	69	theme	Cell	142:145	arg1	glycans					166:172	Cell surface-associated glycans	142:172	Cell surface-associated glycans	142:172	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	4	70	theme	Whole-organism	560:573	arg1	analysis					584:591	Whole-organism MALDI-MS analysis	560:591	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea	560:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	6	71	theme	methylation	1009:1019	arg1	patterns					1021:1028	extensive, yet selective, methylation patterns	983:1028	extensive, yet selective, methylation patterns	983:1028	N-Glycans showed extensive, yet selective, methylation patterns, ranging from non-methylated to polymethylated glycoforms.					
29475940	4	72	with	presence	677:684	arg1	structures					818:827	polygalactosylated (n = 3-5) core fucose structures	777:827	polygalactosylated (n = 3-5) core fucose structures	777:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	72	with	presence	677:684	arg1	mono-					761:765	unusual mono-	753:765	unusual mono-	753:765	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	72	with	presence	677:684	arg1	di-					768:770	di-	768:770	di-	768:770	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	13	73	theme	GlcNAc-independent	2136:2153	arg1	pathway					2168:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	a GlcNAc-independent biosynthetic pathway	2134:2174	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	0	74	theme	N-glycans	91:99	arg1	Identification					0:13	Identification	0:13	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.	0:140	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	9	75	theme	potential	1415:1423	arg1	homologs					1425:1432	two potential homologs	1411:1432	two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1411:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	1	76	theme	many	182:185	arg1	organization					274:285	extracellular matrix organization	253:285	extracellular matrix organization	253:285	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	76	theme	many	182:185	arg1	signaling					238:246	signaling	238:246	signaling	238:246	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	76	theme	many	182:185	arg1	migration					227:235	migration	227:235	migration	227:235	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	76	theme	many	182:185	arg1	processes					196:204	many cellular processes	182:204	many cellular processes	182:204	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	1	76	theme	many	182:185	arg1	adhesion					217:224	adhesion	217:224	adhesion	217:224	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	4	77	theme	MALDI-MS	575:582	arg1	analysis					584:591	Whole-organism MALDI-MS analysis	560:591	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea	560:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	4	78	theme	polygalactosylated	777:794	arg1	structures					818:827	polygalactosylated (n = 3-5) core fucose structures	777:827	polygalactosylated (n = 3-5) core fucose structures	777:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	0	79	theme	Schmidtea	118:126	arg1	mediterranea					128:139	the planarian Schmidtea mediterranea	104:139	the planarian Schmidtea mediterranea	104:139	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	9	80	theme	Galβ1-4Fuc-synthesizing	1441:1463	arg1	enzyme					1465:1470	the Galβ1-4Fuc-synthesizing enzyme	1437:1470	the Galβ1-4Fuc-synthesizing enzyme	1437:1470	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	13	81	from	presence	2122:2129	arg1	mediterranea					2182:2193	S. mediterranea	2179:2193	S. mediterranea	2179:2193	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	10	82	theme	GALT-1	1614:1619	arg1	homologs					1621:1628	two GALT-1 homologs	1610:1628	two GALT-1 homologs	1610:1628	The presence of two GALT-1 homologs suggests different requirements for mono- and polygalactosylation of core fucose for the formation of multiple isomers.					
29475940	1	83	theme	matrix	267:272	arg1	organization					274:285	extracellular matrix organization	253:285	extracellular matrix organization	253:285	Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization.					
29475940	13	84	theme	N-glycan	2084:2091	arg1	biosynthesis					2093:2104	N-glycan biosynthesis	2084:2104	N-glycan biosynthesis	2084:2104	These results contribute greatly to our understanding of N-glycan biosynthesis and suggest the presence of a GlcNAc-independent biosynthetic pathway in S. mediterranea.					
29475940	4	85	theme	core	806:809	arg1	structures					818:827	polygalactosylated (n = 3-5) core fucose structures	777:827	polygalactosylated (n = 3-5) core fucose structures	777:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	0	86	theme	isomeric	27:34	arg1	high-mannose					61:72	multiple isomeric core chitobiose-modified high-mannose	18:72	multiple isomeric core chitobiose-modified high-mannose	18:72	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	4	87	theme	Schmidtea	641:649	arg1	mediterranea					651:662	the planarian Schmidtea mediterranea	627:662	the planarian Schmidtea mediterranea	627:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	0	88	theme	chitobiose-modified	41:59	arg1	high-mannose					61:72	multiple isomeric core chitobiose-modified high-mannose	18:72	multiple isomeric core chitobiose-modified high-mannose	18:72	Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N-glycans in the planarian Schmidtea mediterranea.					
29475940	4	89	theme	other	878:882	arg1	systems					884:890	other systems	878:890	other systems	878:890	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	3	90	theme	glycans	509:515	arg1	structures					489:498	the structures	485:498	the structures of these glycans	485:515	Although uninvestigated, the structures of these glycans may play a role in planarian regeneration.					
29475940	4	91	theme	oligosaccharides	605:620	arg1	analysis					584:591	Whole-organism MALDI-MS analysis	560:591	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea	560:662	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	3	92	theme	planarian	536:544	arg1	regeneration					546:557	planarian regeneration	536:557	planarian regeneration	536:557	Although uninvestigated, the structures of these glycans may play a role in planarian regeneration.					
29475940	9	93	theme	prepharyngeal	1523:1535	arg1	regions					1566:1572	the prepharyngeal, pharyngeal, and mesenchymal regions	1519:1572	the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1519:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	8	94	theme	core	1236:1239	arg1	fucose					1241:1246	monogalactosylated core fucose	1217:1246	monogalactosylated core fucose	1217:1246	Remarkably, monogalactosylated core fucose remained unmethylated, whereas its polygalactosylated forms were methylated, indicating structurally selective methylation.					
29475940	4	95	theme	multiple	689:696	arg1	high-mannose					707:718	multiple isomeric high-mannose and paucimannose structures	689:746	high-mannose	707:718	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	11	96	theme	fucose	1894:1899	arg1	glycosylation					1901:1913	fucose glycosylation	1894:1913	fucose glycosylation	1894:1913	Furthermore, we observed variations in core fucose glycosylation patterns in different planarian strains, suggesting evolutionary adaptation in fucose glycosylation.					
29475940	2	97	theme	complex-type	360:371	arg1	N-glycans					373:381	complex-type N-glycans	360:381	complex-type N-glycans	360:381	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	9	98	theme	pharyngeal	1538:1547	arg1	regions					1566:1572	the prepharyngeal, pharyngeal, and mesenchymal regions	1519:1572	the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea	1519:1591	Using database searches, we identified two potential homologs of the Galβ1-4Fuc-synthesizing enzyme from nematodes (GALT-1) that were expressed in the prepharyngeal, pharyngeal, and mesenchymal regions in S. mediterranea.					
29475940	12	99	theme	different	1986:1994	arg1	glycoforms					1996:2005	>60 different glycoforms	1982:2005	>60 different glycoforms	1982:2005	The various core chitobiose modifications and methylations create >60 different glycoforms in S. mediterranea.					
29475940	4	100	theme	high-mannose	707:718	arg1	presence					677:684	the presence	673:684	the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures	673:827	Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems.					
29475940	2	101	theme	organisms	415:423	arg1	galactosylation					292:306	The galactosylation	288:306	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans	288:381	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
29475940	2	101	theme	organisms	415:423	arg1	characteristic					386:399	characteristic	386:399	characteristic of protostome organisms, including flatworms (planarians)	386:457	The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians).					
30002445	6	0	theme	LLOs	1163:1166	arg1	use					1108:1110	the use	1104:1110	the use of multiple distinct OSTs and structurally diverse LLOs	1104:1166	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	3	1	theme	synthesis	573:581	arg1	technology					591:600	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	3	2	link	asparagine-linked	655:671	arg1	glycosylation					681:693	asparagine-linked protein glycosylation	655:693	asparagine-linked protein glycosylation	655:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	6	3	theme	CFGpS	1061:1065	arg1	platform					1067:1074	The CFGpS platform	1057:1074	The CFGpS platform	1057:1074	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	6	3	theme	CFGpS	1061:1065	arg1	modular					1086:1092	modular	1086:1092	modular	1086:1092	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	5	4	theme	proteins	1047:1054	arg1	glycosylation					1023:1035	efficient and site-specific glycosylation	995:1035	efficient and site-specific glycosylation of target proteins	995:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	7	5	theme	possible	1265:1272	arg1	applications					1274:1285	possible applications	1265:1285	possible applications in on demand biomanufacturing of glycoproteins	1265:1332	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	2	6	gly	glycoproteins	340:352	arg1	glycoproteins					340:352	homogeneous glycoproteins	328:352	homogeneous glycoproteins	328:352	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	5	7	gly	glycosylation	1023:1035	arg1	proteins					1047:1054	target proteins	1040:1054	target proteins	1040:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	4	8	theme	lipid-linked	897:908	arg1	LLOs					928:931	LLOs	928:931	LLOs	928:931	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	4	8	theme	lipid-linked	897:908	arg1	oligosaccharides					910:925	lipid-linked oligosaccharides	897:925	lipid-linked oligosaccharides (LLOs)	897:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	6	9	theme	OSTs	1133:1136	arg1	use					1108:1110	the use	1104:1110	the use of multiple distinct OSTs and structurally diverse LLOs	1104:1166	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	3	10	gly	glycoprotein	560:571	arg1	glycoprotein					560:571	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	3	11	theme	protein	629:635	arg1	biosynthesis					637:648	protein biosynthesis	629:648	protein biosynthesis	629:648	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	2	12	theme	glycoproteins	340:352	arg1	production					314:323	cell-based production	303:323	cell-based production of homogeneous glycoproteins	303:352	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	6	13	theme	distinct	1124:1131	arg1	OSTs					1133:1136	multiple distinct OSTs	1115:1136	multiple distinct OSTs	1115:1136	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	5	14	theme	reaction	975:982	arg1	scheme					984:989	a one-pot reaction scheme	965:989	a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins	965:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	4	15	theme	Escherichia	740:750	arg1	strain					757:762	a glyco-optimized Escherichia coli strain	722:762	a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs)	722:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	2	16	theme	homogeneous	328:338	arg1	glycoproteins					340:352	homogeneous glycoproteins	328:352	homogeneous glycoproteins	328:352	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	5	17	theme	site-specific	1009:1021	arg1	glycosylation					1023:1035	efficient and site-specific glycosylation	995:1035	efficient and site-specific glycosylation of target proteins	995:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	4	18	theme	cell	774:777	arg1	extracts					779:786	source cell extracts	767:786	source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs)	767:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	7	19	theme	demand	1293:1298	arg1	biomanufacturing					1300:1315	demand biomanufacturing	1293:1315	demand biomanufacturing of glycoproteins	1293:1332	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	5	20	theme	resulting	939:947	arg1	extracts					949:956	The resulting extracts	935:956	The resulting extracts	935:956	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	1	21	theme	designer	213:220	arg1	glycans					222:228	designer glycans	213:228	designer glycans	213:228	The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics.					
30002445	0	22	theme	glycoprotein	11:22	arg1	biosynthesis					24:35	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	0	23	theme	glycosylation	102:114	arg1	machinery					116:124	glycosylation machinery	102:124	glycosylation machinery	102:124	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	7	24	from	understanding	1226:1238	arg1	glycoscience					1243:1254	glycoscience	1243:1254	glycoscience	1243:1254	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	2	25	theme	due	386:388	arg1	challenge					376:384	a significant challenge	362:384	a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo	362:498	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	2	26	theme	glycosylation	449:461	arg1	components					463:472	glycosylation components	449:472	glycosylation components	449:472	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	0	27	theme	Single-pot	0:9	arg1	biosynthesis					24:35	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	6	28	theme	multiple	1115:1122	arg1	OSTs					1133:1136	multiple distinct OSTs	1115:1136	multiple distinct OSTs	1115:1136	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	3	29	theme	asparagine-linked	655:671	arg1	glycosylation					681:693	asparagine-linked protein glycosylation	655:693	asparagine-linked protein glycosylation	655:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	7	30	theme	glycoproteins	1320:1332	arg1	biomanufacturing					1300:1315	demand biomanufacturing	1293:1315	demand biomanufacturing of glycoproteins	1293:1332	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	2	31	theme	significant	364:374	arg1	challenge					376:384	a significant challenge	362:384	a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo	362:498	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	5	32	theme	one-pot	967:973	arg1	scheme					984:989	a one-pot reaction scheme	965:989	a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins	965:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	7	33	theme	fundamental	1214:1224	arg1	understanding					1226:1238	fundamental understanding	1214:1238	fundamental understanding in glycoscience	1214:1254	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	3	34	theme	glycoprotein	560:571	arg1	technology					591:600	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	4	35	link	lipid-linked	897:908	arg1	LLOs					928:931	LLOs	928:931	LLOs	928:931	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	4	35	link	lipid-linked	897:908	arg1	oligosaccharides					910:925	lipid-linked oligosaccharides	897:925	lipid-linked oligosaccharides (LLOs)	897:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	0	36	theme	cell-free	45:53	arg1	system					81:86	a cell-free transcription-translation system	43:86	a cell-free transcription-translation system enriched with glycosylation machinery	43:124	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	2	37	theme	viability	398:406	arg1	constraints					408:418	cell viability constraints	393:418	cell viability constraints	393:418	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	5	38	theme	efficient	995:1003	arg1	glycosylation					1023:1035	efficient and site-specific glycosylation	995:1035	efficient and site-specific glycosylation of target proteins	995:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	0	39	gly	glycoprotein	11:22	arg1	glycoprotein					11:22	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis	0:35	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	2	40	theme	precise	477:483	arg1	ratios					485:490	precise ratios	477:490	precise ratios	477:490	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	3	41	theme	protein	673:679	arg1	glycosylation					681:693	asparagine-linked protein glycosylation	655:693	asparagine-linked protein glycosylation	655:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	3	42	theme	cell-free	550:558	arg1	technology					591:600	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	0	43	theme	transcription-translation	55:79	arg1	system					81:86	a cell-free transcription-translation system	43:86	a cell-free transcription-translation system enriched with glycosylation machinery	43:124	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.					
30002445	7	44	from	applications	1274:1285	arg1	biomanufacturing					1300:1315	demand biomanufacturing	1293:1315	demand biomanufacturing of glycoproteins	1293:1332	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	4	45	dep	Escherichia	740:750	arg1	coli					752:755	coli	752:755	coli	752:755	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	1	46	theme	bacterial	154:162	arg1	glycoengineering					164:179	bacterial glycoengineering	154:179	bacterial glycoengineering	154:179	The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics.					
30002445	7	47	gly	glycoproteins	1320:1332	arg1	glycoproteins					1320:1332	glycoproteins	1320:1332	glycoproteins	1320:1332	As such, we anticipate CFGpS will facilitate fundamental understanding in glycoscience and make possible applications in on demand biomanufacturing of glycoproteins.					
30002445	1	48	theme	glycoengineering	164:179	arg1	discipline					140:149	The emerging discipline	127:149	The emerging discipline of bacterial glycoengineering	127:179	The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics.					
30002445	1	49	theme	emerging	131:138	arg1	discipline					140:149	The emerging discipline	127:149	The emerging discipline of bacterial glycoengineering	127:179	The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics.					
30002445	2	50	theme	cell-based	303:312	arg1	production					314:323	cell-based production	303:323	cell-based production of homogeneous glycoproteins	303:352	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	3	51	theme	novel	544:548	arg1	technology					591:600	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	2	52	theme	cell	393:396	arg1	constraints					408:418	cell viability constraints	393:418	cell viability constraints	393:418	Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo.					
30002445	3	53	theme	CFGpS	584:588	arg1	technology					591:600	a novel cell-free glycoprotein synthesis (CFGpS) technology	542:600	a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation	542:693	To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation.					
30002445	4	54	theme	source	767:772	arg1	extracts					779:786	source cell extracts	767:786	source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs)	767:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	4	55	theme	glyco-optimized	724:738	arg1	strain					757:762	a glyco-optimized Escherichia coli strain	722:762	a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs)	722:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	6	56	theme	diverse	1155:1161	arg1	LLOs					1163:1166	structurally diverse LLOs	1142:1166	structurally diverse LLOs	1142:1166	The CFGpS platform is highly modular, allowing the use of multiple distinct OSTs and structurally diverse LLOs.					
30002445	5	57	theme	target	1040:1045	arg1	proteins					1047:1054	target proteins	1040:1054	target proteins	1040:1054	The resulting extracts enable a one-pot reaction scheme for efficient and site-specific glycosylation of target proteins.					
30002445	4	58	theme	glycosylation	823:835	arg1	components					837:846	glycosylation components	823:846	glycosylation components	823:846	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	4	58	theme	glycosylation	823:835	arg1	oligosaccharyltransferases					859:884	oligosaccharyltransferases	859:884	oligosaccharyltransferases (OSTs)	859:891	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30002445	4	58	theme	glycosylation	823:835	arg1	oligosaccharides					910:925	lipid-linked oligosaccharides	897:925	lipid-linked oligosaccharides (LLOs)	897:932	This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs).					
30737276	6	0	theme	antigenic	985:993	arg1	variants					995:1002	antigenic variants	985:1002	antigenic variants having truncated glycan structures	985:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	8	1	theme	N-glycan	1476:1483	arg1	region					1490:1495	the conserved N-glycan core region	1462:1495	the conserved N-glycan core region	1462:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	10	2	theme	residues	1703:1710	arg1	four					1686:1689	four	1686:1689	four	1686:1689	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	10	2	theme	residues	1703:1710	arg1	N-glycans					1730:1738	the PBCV-1 MCP N-glycans	1715:1738	the PBCV-1 MCP N-glycans	1715:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	10	2	theme	residues	1703:1710	arg1	residues					1703:1710	the nine residues	1694:1710	the nine residues of the PBCV-1 MCP N-glycans	1694:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	7	3	theme	Rha	1322:1324	arg1	unit					1327:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	8	4	theme	region	1490:1495	arg1	l-fucose					1431:1438	the l-fucose	1427:1438	the l-fucose (Fuc) that is part of the conserved N-glycan core region	1427:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	8	4	theme	region	1490:1495	arg1	part					1454:1457	part	1454:1457	part of the conserved N-glycan core region	1454:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	1	5	theme	Chlorella	263:271	arg1	NC64A					284:288	the microalga Chlorella variabilis NC64A	249:288	the microalga Chlorella variabilis NC64A	249:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	7	6	theme	α-l-rhamnose	1308:1319	arg1	unit					1327:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	6	7	theme	gene	959:962	arg1	mutants					964:970	PBCV-1 spontaneous GT gene mutants	937:970	PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	937:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	10	8	theme	MCP	1726:1728	arg1	N-glycans					1730:1738	the PBCV-1 MCP N-glycans	1715:1738	the PBCV-1 MCP N-glycans	1715:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	5	9	theme	third	792:796	arg1	GT					798:799	the third GT	788:799	the third GT	788:799	Two of the three GTs were previously annotated as GTs, but the third GT was identified in this study.					
30737276	2	10	theme	most	298:301	arg1	viruses					309:315	most other viruses	298:315	most other viruses	298:315	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	6	11	theme	GT	849:850	arg1	functions					852:860	the GT functions	845:860	the GT functions	845:860	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	7	12	from	positions	1282:1290	arg1	unit					1327:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	7	13	with	GT	1103:1104	arg1	domains					1117:1123	three domains	1111:1123	three domains	1111:1123	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	9	14	theme	semiconserved	1568:1580	arg1	α-d-Rha					1591:1597	α-d-Rha	1591:1597	α-d-Rha	1591:1597	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	9	14	theme	semiconserved	1568:1580	arg1	element					1582:1588	a semiconserved element	1566:1588	a semiconserved element	1566:1588	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	6	15	contain	having	1004:1009	arg1	variants					995:1002	antigenic variants	985:1002	antigenic variants having truncated glycan structures	985:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	6	15	contain	having	1004:1009	arg2	structures					1028:1037	truncated glycan structures	1011:1037	truncated glycan structures	1011:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	0	16	theme	chlorovirus	53:63	arg1	protein					85:91	chlorovirus PBCV-1 major capsid protein	53:91	chlorovirus PBCV-1 major capsid protein	53:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	9	17	theme	core	1625:1628	arg1	region					1630:1635	the core region	1621:1635	the core region	1621:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	1	18	theme	chlorovirus	154:164	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	18	theme	chlorovirus	154:164	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	18	theme	chlorovirus	154:164	arg1	PBCV-1					205:210	PBCV-1	205:210	PBCV-1	205:210	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	4	19	theme	virus-encoded	629:641	arg1	GTs					665:667	GTs	665:667	GTs	665:667	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	4	19	theme	virus-encoded	629:641	arg1	glycosyltransferases					643:662	three virus-encoded glycosyltransferases	623:662	three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis	623:726	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	0	20	theme	major	72:76	arg1	protein					85:91	chlorovirus PBCV-1 major capsid protein	53:91	chlorovirus PBCV-1 major capsid protein	53:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	7	21	contain	has	1135:1137	arg2	activity					1166:1173	a β-l-rhamnosyltransferase activity	1139:1173	a β-l-rhamnosyltransferase activity	1139:1173	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	7	21	contain	has	1135:1137	arg1	domain					1126:1131	domain 1	1126:1133	domain 1	1126:1133	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	1	22	theme	bursaria	177:184	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	22	theme	bursaria	177:184	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	22	theme	bursaria	177:184	arg1	PBCV-1					205:210	PBCV-1	205:210	PBCV-1	205:210	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	2	23	dep	not	342:344	arg1	if					339:340	if	339:340	if	339:340	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	0	24	theme	protein	85:91	arg1	variants					41:48	the antigenic variants	27:48	the antigenic variants of chlorovirus PBCV-1 major capsid protein	27:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	6	25	theme	glycan	882:887	arg1	structures					889:898	the WT glycan structures	875:898	the WT glycan structures from PBCV-1	875:910	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	8	26	theme	d-xylose	1404:1411	arg1	unit					1419:1422	the distal d-xylose (Xyl) unit	1393:1422	the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region	1393:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	4	27	theme	glycan	711:716	arg1	synthesis					718:726	glycan synthesis	711:726	glycan synthesis	711:726	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	6	28	theme	mutants	964:970	arg1	set					930:932	a set	928:932	a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	928:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	6	28	theme	mutants	964:970	arg1	mutants					964:970	PBCV-1 spontaneous GT gene mutants	937:970	PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	937:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	8	29	theme	Xyl	1414:1416	arg1	unit					1419:1422	the distal d-xylose (Xyl) unit	1393:1422	the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region	1393:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	6	30	from	PBCV-1	905:910	arg1	structures					889:898	the WT glycan structures	875:898	the WT glycan structures from PBCV-1	875:910	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	7	31	theme	virus	1076:1080	arg1	a064r					1087:1091	the virus gene a064r	1072:1091	the virus gene a064r	1072:1091	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	7	31	theme	virus	1076:1080	arg1	GT					1103:1104	a GT	1101:1104	a GT with three domains	1101:1123	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	1	32	theme	variabilis	273:282	arg1	NC64A					284:288	the microalga Chlorella variabilis NC64A	249:288	the microalga Chlorella variabilis NC64A	249:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	6	33	theme	GT	956:957	arg1	mutants					964:970	PBCV-1 spontaneous GT gene mutants	937:970	PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	937:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	4	34	theme	distinct	685:692	arg1	activities					697:706	four distinct GT activities	680:706	four distinct GT activities	680:706	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	2	35	theme	major	396:400	arg1	MCP					418:420	MCP	418:420	MCP	418:420	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	2	35	theme	major	396:400	arg1	protein					409:415	its major capsid protein	392:415	its major capsid protein (MCP)	392:421	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	6	36	theme	PBCV-1	937:942	arg1	mutants					964:970	PBCV-1 spontaneous GT gene mutants	937:970	PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	937:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	7	37	theme	working	1057:1063	arg1	model					1065:1069	our working model	1053:1069	our working model	1053:1069	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	3	38	theme	life	584:587	arg1	life					584:587	life	584:587	life	584:587	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	3	38	theme	life	584:587	arg1	domains					573:579	the three domains	563:579	the three domains of life	563:587	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	0	39	theme	variants	41:48	arg1	structures					13:22	The N-glycan structures	0:22	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein	0:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	3	40	from	structures	428:437	arg1	MCP					484:486	the PBCV-1 MCP	473:486	the PBCV-1 MCP	473:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	1	41	theme	large	218:222	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	41	theme	large	218:222	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	7	42	theme	β-l-rhamnosyltransferase	1141:1164	arg1	activity					1166:1173	a β-l-rhamnosyltransferase activity	1139:1173	a β-l-rhamnosyltransferase activity	1139:1173	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	9	43	attach	attachment	1552:1561	arg1	l-Fuc					1612:1616	the same l-Fuc	1603:1616	the same l-Fuc in the core region	1603:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	9	43	attach	attachment	1552:1561	arg2	α-d-Rha					1591:1597	α-d-Rha	1591:1597	α-d-Rha	1591:1597	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	9	43	attach	attachment	1552:1561	arg2	element					1582:1588	a semiconserved element	1566:1588	a semiconserved element	1566:1588	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	3	44	theme	glycans	460:466	arg1	structures					428:437	The structures	424:437	The structures of the four N-linked glycans from the PBCV-1 MCP	424:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	3	45	located	found	544:548	arg2	glycans					528:534	similar glycans	520:534	similar glycans	520:534	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	3	45	located	found	544:548	arg1	life					584:587	life	584:587	life	584:587	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	3	45	located	found	544:548	arg1	domains					573:579	the three domains	563:579	the three domains of life	563:587	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	8	46	theme	conserved	1466:1474	arg1	region					1490:1495	the conserved N-glycan core region	1462:1495	the conserved N-glycan core region	1462:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	1	47	theme	microalga	253:261	arg1	NC64A					284:288	the microalga Chlorella variabilis NC64A	249:288	the microalga Chlorella variabilis NC64A	249:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	9	48	theme	gene	1504:1507	arg1	a071r					1509:1513	gene a071r	1504:1513	gene a071r	1504:1513	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	9	48	theme	gene	1504:1507	arg1	GT					1525:1526	a GT	1523:1526	a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region	1523:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	8	49	theme	core	1485:1488	arg1	region					1490:1495	the conserved N-glycan core region	1462:1495	the conserved N-glycan core region	1462:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	10	50	theme	N-glycans	1730:1738	arg1	N-glycans					1730:1738	the PBCV-1 MCP N-glycans	1715:1738	the PBCV-1 MCP N-glycans	1715:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	10	50	theme	N-glycans	1730:1738	arg1	residues					1703:1710	the nine residues	1694:1710	the nine residues of the PBCV-1 MCP N-glycans	1694:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	1	51	theme	chlorella	186:194	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	51	theme	chlorella	186:194	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	51	theme	chlorella	186:194	arg1	PBCV-1					205:210	PBCV-1	205:210	PBCV-1	205:210	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	9	52	from	l-Fuc	1612:1616	arg1	region					1630:1635	the core region	1621:1635	the core region	1621:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	10	53	theme	PBCV-1	1719:1724	arg1	N-glycans					1730:1738	the PBCV-1 MCP N-glycans	1715:1738	the PBCV-1 MCP N-glycans	1715:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	3	54	theme	similar	520:526	arg1	glycans					528:534	similar glycans	520:534	similar glycans	520:534	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	6	55	theme	WT	879:880	arg1	structures					889:898	the WT glycan structures	875:898	the WT glycan structures from PBCV-1	875:910	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	7	56	theme	terminal	1299:1306	arg1	unit					1327:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	the terminal α-l-rhamnose (Rha) unit	1295:1330	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	0	57	theme	virus-encoded	114:126	arg1	glycosyltransferases					128:147	the virus-encoded glycosyltransferases	110:147	the virus-encoded glycosyltransferases	110:147	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	2	58	theme	other	303:307	arg1	viruses					309:315	most other viruses	298:315	most other viruses	298:315	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	4	59	contain	have	675:678	arg2	activities					697:706	four distinct GT activities	680:706	four distinct GT activities	680:706	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	4	59	contain	have	675:678	arg1	GTs					665:667	GTs	665:667	GTs	665:667	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	4	59	contain	have	675:678	arg1	glycosyltransferases					643:662	three virus-encoded glycosyltransferases	623:662	three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis	623:726	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	8	60	theme	a075l	1337:1341	arg1	gene					1343:1346	The a075l gene	1333:1346	The a075l gene	1333:1346	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	8	60	theme	a075l	1337:1341	arg1	β-xylosyltransferase					1358:1377	a β-xylosyltransferase	1356:1377	a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region	1356:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	7	61	contain	has	1185:1187	arg1	domain					1176:1181	domain 2	1176:1183	domain 2	1176:1183	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	7	61	contain	has	1185:1187	arg2	activity					1217:1224	an α-l-rhamnosyltransferase activity	1189:1224	an α-l-rhamnosyltransferase activity	1189:1224	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	4	62	theme	glycosyltransferases	643:662	arg1	roles					614:618	the roles	610:618	the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis	610:726	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	3	63	link	N-linked	451:458	arg1	glycans					460:466	the four N-linked glycans	442:466	the four N-linked glycans from the PBCV-1 MCP	442:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	0	64	theme	PBCV-1	65:70	arg1	protein					85:91	chlorovirus PBCV-1 major capsid protein	53:91	chlorovirus PBCV-1 major capsid protein	53:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	9	65	theme	element	1582:1588	arg1	attachment					1552:1561	the attachment	1548:1561	the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region	1548:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	3	66	from	MCP	484:486	arg1	glycans					460:466	the four N-linked glycans	442:466	the four N-linked glycans from the PBCV-1 MCP	442:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	3	66	from	MCP	484:486	arg1	structures					428:437	The structures	424:437	The structures of the four N-linked glycans from the PBCV-1 MCP	424:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	0	67	theme	capsid	78:83	arg1	protein					85:91	chlorovirus PBCV-1 major capsid protein	53:91	chlorovirus PBCV-1 major capsid protein	53:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	1	68	theme	Paramecium	166:175	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	68	theme	Paramecium	166:175	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	68	theme	Paramecium	166:175	arg1	PBCV-1					205:210	PBCV-1	205:210	PBCV-1	205:210	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	8	69	theme	distal	1397:1402	arg1	unit					1419:1422	the distal d-xylose (Xyl) unit	1393:1422	the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region	1393:1495	The a075l gene encodes a β-xylosyltransferase that attaches the distal d-xylose (Xyl) unit to the l-fucose (Fuc) that is part of the conserved N-glycan core region.					
30737276	2	70	gly	glycosylate	380:390	arg1	MCP					418:420	MCP	418:420	MCP	418:420	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	2	70	gly	glycosylate	380:390	arg1	protein					409:415	its major capsid protein	392:415	its major capsid protein (MCP)	392:421	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	4	71	theme	GT	694:695	arg1	activities					697:706	four distinct GT activities	680:706	four distinct GT activities	680:706	Here, we identified the roles of three virus-encoded glycosyltransferases (GTs) that have four distinct GT activities in glycan synthesis.					
30737276	6	72	theme	truncated	1011:1019	arg1	structures					1028:1037	truncated glycan structures	1011:1037	truncated glycan structures	1011:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	7	73	theme	gene	1082:1085	arg1	a064r					1087:1091	the virus gene a064r	1072:1091	the virus gene a064r	1072:1091	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	7	73	theme	gene	1082:1085	arg1	GT					1103:1104	a GT	1101:1104	a GT with three domains	1101:1123	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	9	74	theme	same	1607:1610	arg1	l-Fuc					1612:1616	the same l-Fuc	1603:1616	the same l-Fuc in the core region	1603:1635	Last, gene a071r encodes a GT that is involved in the attachment of a semiconserved element, α-d-Rha, to the same l-Fuc in the core region.					
30737276	0	75	theme	N-glycan	4:11	arg1	structures					13:22	The N-glycan structures	0:22	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein	0:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	7	76	theme	α-l-rhamnosyltransferase	1192:1215	arg1	activity					1217:1224	an α-l-rhamnosyltransferase activity	1189:1224	an α-l-rhamnosyltransferase activity	1189:1224	According to our working model, the virus gene a064r encodes a GT with three domains: domain 1 has a β-l-rhamnosyltransferase activity, domain 2 has an α-l-rhamnosyltransferase activity, and domain 3 is a methyltransferase that decorates two positions in the terminal α-l-rhamnose (Rha) unit.					
30737276	5	77	theme	GTs	746:748	arg1	Two					729:731	Two	729:731	Two	729:731	Two of the three GTs were previously annotated as GTs, but the third GT was identified in this study.					
30737276	5	77	theme	GTs	746:748	arg1	GTs					779:781	GTs	779:781	GTs	779:781	Two of the three GTs were previously annotated as GTs, but the third GT was identified in this study.					
30737276	5	77	theme	GTs	746:748	arg1	GTs					746:748	the three GTs	736:748	the three GTs	736:748	Two of the three GTs were previously annotated as GTs, but the third GT was identified in this study.					
30737276	6	78	theme	spontaneous	944:954	arg1	mutants					964:970	PBCV-1 spontaneous GT gene mutants	937:970	PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures	937:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	2	79	theme	capsid	402:407	arg1	MCP					418:420	MCP	418:420	MCP	418:420	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	2	79	theme	capsid	402:407	arg1	protein					409:415	its major capsid protein	392:415	its major capsid protein (MCP)	392:421	Unlike most other viruses, PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP).					
30737276	0	80	theme	antigenic	31:39	arg1	variants					41:48	the antigenic variants	27:48	the antigenic variants of chlorovirus PBCV-1 major capsid protein	27:91	The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases.					
30737276	10	81	theme	GT	1658:1659	arg1	activities					1661:1670	GT activities	1658:1670	GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans	1658:1738	Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans.					
30737276	3	82	theme	N-linked	451:458	arg1	glycans					460:466	the four N-linked glycans	442:466	the four N-linked glycans from the PBCV-1 MCP	442:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30737276	6	83	theme	glycan	1021:1026	arg1	structures					1028:1037	truncated glycan structures	1011:1037	truncated glycan structures	1011:1037	We determined the GT functions by comparing the WT glycan structures from PBCV-1 with those from a set of PBCV-1 spontaneous GT gene mutants resulting in antigenic variants having truncated glycan structures.					
30737276	1	84	theme	dsDNA	224:228	arg1	virus					230:234	a large dsDNA virus	216:234	a large dsDNA virus that infects the microalga Chlorella variabilis NC64A	216:288	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	1	84	theme	dsDNA	224:228	arg1	virus					196:200	The chlorovirus Paramecium bursaria chlorella virus 1	150:202	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1)	150:211	The chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) is a large dsDNA virus that infects the microalga Chlorella variabilis NC64A.					
30737276	3	85	theme	PBCV-1	477:482	arg1	MCP					484:486	the PBCV-1 MCP	473:486	the PBCV-1 MCP	473:486	The structures of the four N-linked glycans from the PBCV-1 MCP consist of nonasaccharides, and similar glycans are not found elsewhere in the three domains of life.					
30074304	0	0	theme	Fresh	67:71	arg1	Tissue					109:114	Fresh or Formalin-Fixed Paraffin-Embedded Tissue	67:114	Tissue	109:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	6	1	theme	individual	1096:1105	arg1	N-glycans					1107:1115	at least 40 or more individual N-glycans	1076:1115	at least 40 or more individual N-glycans	1076:1115	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	1	2	theme	secreted	148:155	arg1	Glycosylation					117:129	Glycosylation	117:129	Glycosylation of cell surface, secreted, and circulating proteins	117:181	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	1	3	theme	modification	237:248	arg1	types					209:213	the most common types	193:213	the most common types of post-translational modification	193:248	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	5	4	theme	released	879:886	arg1	N-glycans					888:896	all released N-glycans	875:896	all released N-glycans in a given region of tissue	875:924	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	5	theme	laser	961:965	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	5	theme	laser	961:965	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	0	6	theme	Formalin-Fixed	76:89	arg1	Tissue					109:114	Fresh or Formalin-Fixed Paraffin-Embedded Tissue	67:114	Tissue	109:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	2	7	link	N-linked	322:329	arg1	glycosylation					331:343	N-linked glycosylation	322:343	N-linked glycosylation on asparagine residues	322:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	6	8	theme	tissue	1134:1139	arg1	histopathology					1141:1154	tissue histopathology	1134:1154	tissue histopathology	1134:1154	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	3	9	theme	peptide	548:554	arg1	enzyme					540:545	an endoglycosidase enzyme	521:545	an endoglycosidase enzyme	521:545	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	9	theme	peptide	548:554	arg1	F					570:570	peptide N-glycosidase F	548:570	peptide N-glycosidase F (PNGase F)	548:581	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	9	theme	peptide	548:554	arg1	F					580:580	PNGase F	573:580	PNGase F	573:580	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	10	theme	PNGase	573:578	arg1	F					570:570	peptide N-glycosidase F	548:570	peptide N-glycosidase F (PNGase F)	548:581	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	10	theme	PNGase	573:578	arg1	F					580:580	PNGase F	573:580	PNGase F	573:580	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	11	theme	N-glycosidase	556:568	arg1	enzyme					540:545	an endoglycosidase enzyme	521:545	an endoglycosidase enzyme	521:545	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	11	theme	N-glycosidase	556:568	arg1	F					570:570	peptide N-glycosidase F	548:570	peptide N-glycosidase F (PNGase F)	548:581	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	11	theme	N-glycosidase	556:568	arg1	F					580:580	PNGase F	573:580	PNGase F	573:580	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	2	12	from	glycosylation	331:343	arg1	residues					359:366	asparagine residues	348:366	asparagine residues	348:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	13	theme	asparagine	348:357	arg1	residues					359:366	asparagine residues	348:366	asparagine residues	348:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	14	theme	classes	313:319	arg1	classes					313:319	three major classes	301:319	three major classes	301:319	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	14	theme	classes	313:319	arg1	glycosylation					378:390	O-linked glycosylation	369:390	O-linked glycosylation on serine or threonine residues	369:422	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	14	theme	classes	313:319	arg1	one					294:296	one	294:296	one	294:296	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	0	15	theme	Tissue	109:114	arg1	Spectrometry					51:62	MALDI Imaging Mass Spectrometry	32:62	MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue	32:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	2	16	from	polymers	465:472	arg1	serine					477:482	serine	477:482	serine	477:482	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	5	17	theme	matrix-assisted	945:959	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	17	theme	matrix-assisted	945:959	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	6	18	theme	described	1046:1054	arg1	protocol					1066:1073	the described MALDI-IMS protocol	1042:1073	the described MALDI-IMS protocol	1042:1073	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	6	19	theme	structural	1182:1191	arg1	approaches					1202:1211	further structural analysis approaches	1174:1211	further structural analysis approaches	1174:1211	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	0	20	theme	Paraffin-Embedded	91:107	arg1	Tissue					109:114	Fresh or Formalin-Fixed Paraffin-Embedded Tissue	67:114	Tissue	109:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	2	21	theme	glycosaminoglycan	431:447	arg1	polymers					465:472	glycosaminoglycan oligosaccharide polymers	431:472	glycosaminoglycan oligosaccharide polymers on serine	431:482	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	1	22	theme	circulating	162:172	arg1	proteins					174:181	circulating proteins	162:181	circulating proteins	162:181	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	2	23	theme	oligosaccharide	449:463	arg1	polymers					465:472	glycosaminoglycan oligosaccharide polymers	431:472	glycosaminoglycan oligosaccharide polymers on serine	431:482	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	5	24	theme	MALDI	990:994	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	24	theme	MALDI	990:994	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	2	25	theme	major	307:311	arg1	classes					313:319	three major classes	301:319	three major classes	301:319	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	25	theme	major	307:311	arg1	glycosylation					378:390	O-linked glycosylation	369:390	O-linked glycosylation on serine or threonine residues	369:422	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	1	26	theme	proteins	174:181	arg1	Glycosylation					117:129	Glycosylation	117:129	Glycosylation of cell surface, secreted, and circulating proteins	117:181	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	0	27	theme	In	0:1	arg1	Imaging					8:14	In Situ Imaging	0:14	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.	0:115	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	6	28	theme	further	1174:1180	arg1	approaches					1202:1211	further structural analysis approaches	1174:1211	further structural analysis approaches	1174:1211	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	2	29	gly	glycosylation	378:390	arg1	threonine					405:413	threonine	405:413	threonine	405:413	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	29	gly	glycosylation	378:390	arg1	serine					395:400	serine	395:400	serine	395:400	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	5	30	from	N-glycans	888:896	arg1	region					909:914	a given region	901:914	a given region of tissue	901:924	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	2	31	dep	classes	313:319	arg1	glycosylation					331:343	N-linked glycosylation	322:343	N-linked glycosylation on asparagine residues	322:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	32	theme	O-linked	369:376	arg1	classes					313:319	three major classes	301:319	three major classes	301:319	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	32	theme	O-linked	369:376	arg1	glycosylation					378:390	O-linked glycosylation	369:390	O-linked glycosylation on serine or threonine residues	369:422	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	5	33	theme	solvent	798:804	arg1	sprayer					806:812	solvent sprayer	798:812	solvent sprayer	798:812	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	3	34	theme	attached	596:603	arg1	oligosaccharides					605:620	the attached oligosaccharides	592:620	the attached oligosaccharides between the asparagine and first sugar	592:659	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	1	35	gly	Glycosylation	117:129	arg1	proteins					174:181	circulating proteins	162:181	circulating proteins	162:181	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	1	35	gly	Glycosylation	117:129	arg1	secreted					148:155	secreted	148:155	secreted	148:155	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	1	35	gly	Glycosylation	117:129	arg1	surface					139:145	cell surface	134:145	cell surface	134:145	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	5	36	theme	given	903:907	arg1	region					909:914	a given region	901:914	a given region of tissue	901:924	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	37	theme	tissue	919:924	arg1	region					909:914	a given region	901:914	a given region of tissue	901:924	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	6	38	theme	MALDI-IMS	1056:1064	arg1	protocol					1066:1073	the described MALDI-IMS protocol	1042:1073	the described MALDI-IMS protocol	1042:1073	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	3	39	theme	first	649:653	arg1	sugar					655:659	first sugar	649:659	first sugar	649:659	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	7	40	theme	John	1224:1227	arg1	Sons					1237:1240	John Wiley & Sons	1224:1240	John Wiley & Sons	1224:1240	© 2018 by John Wiley & Sons, Inc.					
30074304	4	41	theme	released	682:689	arg1	N-glycans					691:699	released N-glycans	682:699	released N-glycans	682:699	A method to analyze released N-glycans and map them to specific locations within a tissue is presented here.					
30074304	3	42	theme	N-linked	503:510	arg1	glycans					512:518	N-linked glycans	503:518	N-linked glycans	503:518	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	2	43	link	O-linked	369:376	arg1	classes					313:319	three major classes	301:319	three major classes	301:319	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	43	link	O-linked	369:376	arg1	glycosylation					378:390	O-linked glycosylation	369:390	O-linked glycosylation on serine or threonine residues	369:422	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	5	44	theme	imaging	997:1003	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	44	theme	imaging	997:1003	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	45	theme	formalin-fixed	848:861	arg1	tissues					863:869	frozen or formalin-fixed tissues	838:869	frozen or formalin-fixed tissues	838:869	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	46	theme	molecular	819:827	arg1	F					782:782	The PNGase F	771:782	The PNGase F	771:782	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	46	theme	molecular	819:827	arg1	layer					829:833	a molecular layer	817:833	a molecular layer on frozen or formalin-fixed tissues	817:869	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	0	47	theme	N-Glycans	19:27	arg1	Imaging					8:14	In Situ Imaging	0:14	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.	0:115	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	7	48	theme	Wiley	1229:1233	arg1	Sons					1237:1240	John Wiley & Sons	1224:1240	John Wiley & Sons	1224:1240	© 2018 by John Wiley & Sons, Inc.					
30074304	5	49	theme	PNGase	775:780	arg1	F					782:782	The PNGase F	771:782	The PNGase F	771:782	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	49	theme	PNGase	775:780	arg1	layer					829:833	a molecular layer	817:833	a molecular layer on frozen or formalin-fixed tissues	817:869	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	50	theme	mass	1005:1008	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	50	theme	mass	1005:1008	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	2	51	from	glycosylation	378:390	arg1	threonine					405:413	threonine	405:413	threonine	405:413	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	51	from	glycosylation	378:390	arg1	serine					395:400	serine	395:400	serine	395:400	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	0	52	theme	Imaging	38:44	arg1	Spectrometry					51:62	MALDI Imaging Mass Spectrometry	32:62	MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue	32:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	5	53	from	layer	829:833	arg1	tissues					863:869	frozen or formalin-fixed tissues	838:869	frozen or formalin-fixed tissues	838:869	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	0	54	theme	MALDI	32:36	arg1	Spectrometry					51:62	MALDI Imaging Mass Spectrometry	32:62	MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue	32:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	2	55	gly	glycosylation	331:343	arg1	residues					359:366	asparagine residues	348:366	asparagine residues	348:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	2	56	theme	N-linked	322:329	arg1	glycosylation					331:343	N-linked glycosylation	322:343	N-linked glycosylation on asparagine residues	322:366	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	7	57	theme	&	1235:1235	arg1	Sons					1237:1240	John Wiley & Sons	1224:1240	John Wiley & Sons	1224:1240	© 2018 by John Wiley & Sons, Inc.					
30074304	6	58	theme	analysis	1193:1200	arg1	approaches					1202:1211	further structural analysis approaches	1174:1211	further structural analysis approaches	1174:1211	Using the described MALDI-IMS protocol, at least 40 or more individual N-glycans can be mapped to tissue histopathology and extracted for further structural analysis approaches.					
30074304	5	59	theme	desorption/ionization	967:987	arg1	MALDI-IMS					1024:1032	MALDI-IMS	1024:1032	MALDI-IMS	1024:1032	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	5	59	theme	desorption/ionization	967:987	arg1	spectrometry					1010:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry	945:1021	matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS)	945:1033	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	4	60	theme	specific	717:724	arg1	locations					726:734	specific locations	717:734	specific locations within a tissue	717:750	A method to analyze released N-glycans and map them to specific locations within a tissue is presented here.					
30074304	3	61	dep	asparagine	634:643	arg1	the					630:632	the	630:632	the	630:632	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	1	62	theme	common	202:207	arg1	types					209:213	the most common types	193:213	the most common types of post-translational modification	193:248	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	3	63	link	N-linked	503:510	arg1	glycans					512:518	N-linked glycans	503:518	N-linked glycans	503:518	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	4	64	dep	method	664:669	arg1	analyze					674:680	analyze	674:680	analyze released N-glycans	674:699	A method to analyze released N-glycans and map them to specific locations within a tissue is presented here.					
30074304	4	64	dep	method	664:669	arg1	map					705:707	map	705:707	map them to specific locations within a tissue	705:750	A method to analyze released N-glycans and map them to specific locations within a tissue is presented here.					
30074304	1	65	theme	cell	134:137	arg1	surface					139:145	cell surface	134:145	cell surface	134:145	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	1	66	theme	types	209:213	arg1	types					209:213	the most common types	193:213	the most common types of post-translational modification	193:248	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	1	66	theme	types	209:213	arg1	one					186:188	one	186:188	one	186:188	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	2	67	dep	serine	395:400	arg1	residues					415:422	residues	415:422	residues	415:422	These modifications occur most commonly as one of three major classes: N-linked glycosylation on asparagine residues, O-linked glycosylation on serine or threonine residues, or as glycosaminoglycan oligosaccharide polymers on serine.					
30074304	1	68	theme	surface	139:145	arg1	Glycosylation					117:129	Glycosylation	117:129	Glycosylation of cell surface, secreted, and circulating proteins	117:181	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30074304	5	69	theme	frozen	838:843	arg1	tissues					863:869	frozen or formalin-fixed tissues	838:869	frozen or formalin-fixed tissues	838:869	The PNGase F is applied by solvent sprayer as a molecular layer on frozen or formalin-fixed tissues and all released N-glycans in a given region of tissue are detected using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (MALDI-IMS).					
30074304	0	70	theme	Mass	46:49	arg1	Spectrometry					51:62	MALDI Imaging Mass Spectrometry	32:62	MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue	32:114	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	0	71	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.					
30074304	3	72	theme	endoglycosidase	524:538	arg1	enzyme					540:545	an endoglycosidase enzyme	521:545	an endoglycosidase enzyme	521:545	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	3	72	theme	endoglycosidase	524:538	arg1	F					570:570	peptide N-glycosidase F	548:570	peptide N-glycosidase F (PNGase F)	548:581	Specifically, for N-linked glycans, an endoglycosidase enzyme, peptide N-glycosidase F (PNGase F), cleaves the attached oligosaccharides between the asparagine and first sugar.					
30074304	1	73	theme	post-translational	218:235	arg1	modification					237:248	post-translational modification	218:248	post-translational modification	218:248	Glycosylation of cell surface, secreted, and circulating proteins is one of the most common types of post-translational modification.					
30621113	2	0	theme	specific	476:483	arg1	activity					494:501	a specific improved activity	474:501	a specific improved activity (i.e., effector function or serum half-life)	474:546	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	5	1	theme	lower	1178:1182	arg1	concentration					1155:1167	a concentration	1153:1167	a concentration 14 times lower than previously published levels	1153:1215	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	4	2	theme	inhibitor	841:849	arg1	kifunensine					851:861	the mannosidase I inhibitor kifunensine	823:861	the mannosidase I inhibitor kifunensine	823:861	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	7	3	theme	transient	1472:1480	arg1	platform					1493:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	7	4	theme	expression	1482:1491	arg1	platform					1493:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	7	5	theme	transgenic	1591:1600	arg1	hosts					1602:1606	transgenic hosts	1591:1606	transgenic hosts	1591:1606	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	4	6	theme	expression	902:911	arg1	platform					913:920	the Nicotiana benthamiana transient expression platform	866:920	the Nicotiana benthamiana transient expression platform	866:920	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	5	7	theme	oligomannose	1125:1136	arg1	glycoforms					1138:1147	oligomannose glycoforms	1125:1147	oligomannose glycoforms	1125:1147	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	4	8	theme	I	839:839	arg1	kifunensine					851:861	the mannosidase I inhibitor kifunensine	823:861	the mannosidase I inhibitor kifunensine	823:861	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	7	9	theme	kifunensine	1514:1524	arg1	use					1507:1509	the use	1503:1509	the use of kifunensine	1503:1524	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	5	10	theme	published	1200:1208	arg1	levels					1210:1215	previously published levels	1189:1215	previously published levels	1189:1215	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	2	11	theme	effector	510:517	arg1	function					519:526	effector function	510:526	effector function	510:526	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	6	12	theme	ADCC	1286:1289	arg1	activity					1291:1298	ADCC activity	1286:1298	ADCC activity	1286:1298	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	6	13	theme	resulting	1222:1230	arg1	rituximab					1245:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	7	14	theme	scalable	1448:1455	arg1	benthamiana					1460:1470	the cost-effective and scalable N. benthamiana	1425:1470	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	1	15	theme	monoclonal	265:274	arg1	antibodies					276:285	monoclonal antibodies	265:285	monoclonal antibodies	265:285	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	2	16	dep	function	519:526	arg1	i.e.					504:507	i.e.	504:507	i.e.	504:507	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	3	17	theme	region	687:692	arg1	N-glycosylation					661:675	the N-glycosylation	657:675	the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies	657:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	7	18	theme	N.	1457:1458	arg1	benthamiana					1460:1470	the cost-effective and scalable N. benthamiana	1425:1470	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	0	19	theme	Rituximab	94:102	arg1	Efficacy					82:89	Efficacy	82:89	Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants	82:147	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	2	20	theme	N-glycosylation	403:417	arg1	either					435:440	either	435:440	either	435:440	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	2	20	theme	N-glycosylation	403:417	arg1	consistent					447:456	consistent	447:456	consistent	447:456	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	2	20	theme	N-glycosylation	403:417	arg1	profile					419:425	a N-glycosylation profile	401:425	a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life)	401:546	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	7	21	theme	cost-effective	1429:1442	arg1	benthamiana					1460:1470	the cost-effective and scalable N. benthamiana	1425:1470	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	4	22	theme	anti-CD20	949:957	arg1	rituximab					969:977	rituximab	969:977	rituximab	969:977	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	4	22	theme	anti-CD20	949:957	arg1	antibody					959:966	an afucosylated anti-CD20 antibody	933:966	an afucosylated anti-CD20 antibody (rituximab)	933:978	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	7	23	theme	benthamiana	1460:1470	arg1	platform					1493:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	the cost-effective and scalable N. benthamiana transient expression platform	1425:1500	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	6	24	theme	14-fold	1266:1272	arg1	increase					1274:1281	a 14-fold increase	1264:1281	a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine	1264:1406	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	5	25	gly	glycoforms	1138:1147	arg1	oligomannose					1125:1136	oligomannose glycoforms	1125:1147	oligomannose glycoforms	1125:1147	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	3	26	theme	Fc	684:685	arg1	region					687:692	the Fc region	680:692	the Fc region of anti-cancer monoclonal antibodies	680:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	4	27	theme	mannosidase	827:837	arg1	kifunensine					851:861	the mannosidase I inhibitor kifunensine	823:861	the mannosidase I inhibitor kifunensine	823:861	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	4	28	theme	afucosylated	936:947	arg1	rituximab					969:977	rituximab	969:977	rituximab	969:977	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	4	28	theme	afucosylated	936:947	arg1	antibody					959:966	an afucosylated anti-CD20 antibody	933:966	an afucosylated anti-CD20 antibody (rituximab)	933:978	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	0	29	theme	In	0:1	arg1	Engineering					15:25	In Vivo Glycan Engineering	0:25	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine)	0:71	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	3	30	theme	antibodies	720:729	arg1	region					687:692	the Fc region	680:692	the Fc region of anti-cancer monoclonal antibodies	680:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	2	31	dep	either	435:440	arg1	either					435:440	either	435:440	either	435:440	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	2	31	dep	either	435:440	arg1	consistent					447:456	consistent	447:456	consistent	447:456	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	2	31	dep	either	435:440	arg1	profile					419:425	a N-glycosylation profile	401:425	a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life)	401:546	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	4	32	gly	afucosylated	936:947	arg1	rituximab					969:977	rituximab	969:977	rituximab	969:977	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	4	32	gly	afucosylated	936:947	arg1	antibody					959:966	an afucosylated anti-CD20 antibody	933:966	an afucosylated anti-CD20 antibody (rituximab)	933:978	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	3	33	theme	antibody-dependent	752:769	arg1	ADCC					799:802	ADCC	799:802	ADCC	799:802	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	3	33	theme	antibody-dependent	752:769	arg1	cytotoxicity					785:796	antibody-dependent cell-mediated cytotoxicity	752:796	antibody-dependent cell-mediated cytotoxicity (ADCC)	752:803	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	1	34	theme	blood	288:292	arg1	factors					294:300	blood factors	288:300	blood factors	288:300	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	0	35	theme	Glycan	8:13	arg1	Engineering					15:25	In Vivo Glycan Engineering	0:25	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine)	0:71	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	3	36	theme	anti-cancer	697:707	arg1	antibodies					720:729	anti-cancer monoclonal antibodies	697:729	anti-cancer monoclonal antibodies	697:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	2	37	theme	serum	531:535	arg1	half-life					537:545	serum half-life	531:545	serum half-life	531:545	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	3	38	gly	N-glycosylation	661:675	arg1	region					687:692	the Fc region	680:692	the Fc region of anti-cancer monoclonal antibodies	680:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	1	39	theme	pharmacokinetic	195:209	arg1	properties					211:220	the pharmacokinetic properties	191:220	the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes	191:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	3	40	theme	monoclonal	709:718	arg1	antibodies					720:729	anti-cancer monoclonal antibodies	697:729	anti-cancer monoclonal antibodies	697:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	0	41	theme	benthamiana	130:140	arg1	Plants					142:147	Nicotiana benthamiana Plants	120:147	Nicotiana benthamiana Plants	120:147	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	6	42	theme	kifunensine	1396:1406	arg1	absence					1385:1391	the absence	1381:1391	the absence of kifunensine	1381:1406	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	0	43	theme	Nicotiana	120:128	arg1	Plants					142:147	Nicotiana benthamiana Plants	120:147	Nicotiana benthamiana Plants	120:147	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	7	44	theme	glycan	1551:1556	arg1	engineering					1558:1568	simple in-process glycan engineering	1533:1568	simple in-process glycan engineering	1533:1568	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	6	45	theme	afucosylated	1232:1243	arg1	rituximab					1245:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	0	46	theme	I	47:47	arg1	Kifunensine					60:70	Kifunensine	60:70	Kifunensine	60:70	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	0	46	theme	I	47:47	arg1	Inhibitor					49:57	the Mannosidase I Inhibitor	31:57	the Mannosidase I Inhibitor (Kifunensine)	31:71	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	1	47	theme	lysosomal	307:315	arg1	enzymes					317:323	lysosomal enzymes	307:323	lysosomal enzymes	307:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	6	48	gly	afucosylated	1232:1243	arg1	rituximab					1245:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab	1218:1253	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	5	49	theme	kifunensine	1024:1034	arg1	concentration					1007:1019	the optimal concentration	995:1019	the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms	995:1147	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	6	50	theme	lymphoma	1314:1321	arg1	line					1328:1331	the lymphoma cell line	1310:1331	the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine	1310:1406	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	0	51	theme	Mannosidase	35:45	arg1	Kifunensine					60:70	Kifunensine	60:70	Kifunensine	60:70	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	0	51	theme	Mannosidase	35:45	arg1	Inhibitor					49:57	the Mannosidase I Inhibitor	31:57	the Mannosidase I Inhibitor (Kifunensine)	31:71	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	6	52	theme	cell	1323:1326	arg1	line					1328:1331	the lymphoma cell line	1310:1331	the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine	1310:1406	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	7	53	theme	simple	1533:1538	arg1	engineering					1558:1568	simple in-process glycan engineering	1533:1568	simple in-process glycan engineering	1533:1568	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	2	54	theme	last	333:336	arg1	decades					342:348	the last two decades	329:348	the last two decades	329:348	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	5	55	theme	optimal	999:1005	arg1	concentration					1007:1019	the optimal concentration	995:1019	the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms	995:1147	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	4	56	theme	transient	892:900	arg1	platform					913:920	the Nicotiana benthamiana transient expression platform	866:920	the Nicotiana benthamiana transient expression platform	866:920	Here, we applied the mannosidase I inhibitor kifunensine to the Nicotiana benthamiana transient expression platform to produce an afucosylated anti-CD20 antibody (rituximab).					
30621113	7	57	theme	in-process	1540:1549	arg1	engineering					1558:1568	simple in-process glycan engineering	1533:1568	simple in-process glycan engineering	1533:1568	When applied to the cost-effective and scalable N. benthamiana transient expression platform, the use of kifunensine allows simple in-process glycan engineering without the need for transgenic hosts.					
30621113	6	58	theme	Wil2-S	1333:1338	arg1	line					1328:1331	the lymphoma cell line	1310:1331	the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine	1310:1406	The resulting afucosylated rituximab revealed a 14-fold increase in ADCC activity targeting the lymphoma cell line Wil2-S when compared with rituximab produced in the absence of kifunensine.					
30621113	1	59	theme	several	225:231	arg1	factors					294:300	blood factors	288:300	blood factors	288:300	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	59	theme	several	225:231	arg1	enzymes					317:323	lysosomal enzymes	307:323	lysosomal enzymes	307:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	59	theme	several	225:231	arg1	classes					233:239	several classes	225:239	several classes	225:239	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	59	theme	several	225:231	arg1	antibodies					276:285	monoclonal antibodies	265:285	monoclonal antibodies	265:285	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	60	gly	N-glycosylation	150:164	arg1	factors					294:300	blood factors	288:300	blood factors	288:300	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	60	gly	N-glycosylation	150:164	arg1	enzymes					317:323	lysosomal enzymes	307:323	lysosomal enzymes	307:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	60	gly	N-glycosylation	150:164	arg1	classes					233:239	several classes	225:239	several classes	225:239	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	60	gly	N-glycosylation	150:164	arg1	antibodies					276:285	monoclonal antibodies	265:285	monoclonal antibodies	265:285	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	5	61	theme	infiltration	1048:1059	arg1	sufficient					1091:1100	sufficient	1091:1100	sufficient	1091:1100	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	5	61	theme	infiltration	1048:1059	arg1	solution					1061:1068	the infiltration solution	1044:1068	the infiltration solution	1044:1068	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	5	61	theme	infiltration	1048:1059	arg1	µM					1077:1078	0.375 µM	1071:1078	0.375 µM	1071:1078	We determined the optimal concentration of kifunensine used in the infiltration solution, 0.375 µM, which was sufficient to produce exclusively oligomannose glycoforms, at a concentration 14 times lower than previously published levels.					
30621113	1	62	theme	classes	233:239	arg1	properties					211:220	the pharmacokinetic properties	191:220	the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes	191:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	3	63	theme	cell-mediated	771:783	arg1	ADCC					799:802	ADCC	799:802	ADCC	799:802	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	3	63	theme	cell-mediated	771:783	arg1	cytotoxicity					785:796	antibody-dependent cell-mediated cytotoxicity	752:796	antibody-dependent cell-mediated cytotoxicity (ADCC)	752:803	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	2	64	theme	improved	485:492	arg1	activity					494:501	a specific improved activity	474:501	a specific improved activity (i.e., effector function or serum half-life)	474:546	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	3	65	theme	N-glycosylation	661:675	arg1	structure					644:652	the structure	640:652	the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies	640:729	In particular, attention has focused on engineering processes in vivo or in vitro to alter the structure of the N-glycosylation of the Fc region of anti-cancer monoclonal antibodies in order to increase antibody-dependent cell-mediated cytotoxicity (ADCC).					
30621113	0	66	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants.					
30621113	2	67	theme	N-glycan	351:358	arg1	engineering					360:370	N-glycan engineering	351:370	N-glycan engineering	351:370	In the last two decades, N-glycan engineering has been employed to achieve a N-glycosylation profile that is either more consistent or aligned with a specific improved activity (i.e., effector function or serum half-life).					
30621113	1	68	theme	biologics	244:252	arg1	factors					294:300	blood factors	288:300	blood factors	288:300	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	68	theme	biologics	244:252	arg1	enzymes					317:323	lysosomal enzymes	307:323	lysosomal enzymes	307:323	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	68	theme	biologics	244:252	arg1	classes					233:239	several classes	225:239	several classes	225:239	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
30621113	1	68	theme	biologics	244:252	arg1	antibodies					276:285	monoclonal antibodies	265:285	monoclonal antibodies	265:285	N-glycosylation has been shown to affect the pharmacokinetic properties of several classes of biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes.					
31141660	6	0	theme	fragment	882:889	arg1	ions					891:894	fragment ions	882:894	fragment ions in N-glycans without a core fucose	882:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	4	1	theme	ion	559:561	arg1	types					563:567	specific ion types	550:567	specific ion types	550:567	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	2	2	theme	methods	262:268	arg1	lack					254:257	a lack	252:257	a lack of methods to discriminate unambiguously the fucose position in one-step	252:330	Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step.					
31141660	1	3	theme	normal	174:179	arg1	processes					198:206	different normal and pathological processes	164:206	different normal and pathological processes	164:206	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	9	4	theme	multifucosylated	1622:1637	arg1	N-glycans					1639:1647	multifucosylated N-glycans	1622:1647	multifucosylated N-glycans detected with lower abundances in lung cancer samples	1622:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	5	5	with	residue	810:816	arg1	Da					842:843	a mass 457 and 441 Da	823:843	Da	842:843	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	5	with	residue	810:816	arg1	mass					825:828	a mass 457 and 441 Da	823:843	mass	825:828	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	1	6	theme	pathological	185:196	arg1	processes					198:206	different normal and pathological processes	164:206	different normal and pathological processes	164:206	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	7	7	from	fucosylation	1220:1231	arg1	recognition					1187:1197	rapid recognition	1181:1197	rapid recognition of antenna from core fucosylation in glycans detected with low abundances	1181:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	7	7	from	fucosylation	1220:1231	arg1	glycans					1236:1242	glycans	1236:1242	glycans detected with low abundances	1236:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	7	8	with	peaks	1108:1112	arg1	intensities					1131:1141	significant intensities	1119:1141	significant intensities	1119:1141	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	6	9	theme	core	919:922	arg1	fucose					924:929	a core fucose	917:929	a core fucose	917:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	9	10	theme	lower	1663:1667	arg1	abundances					1669:1678	lower abundances	1663:1678	lower abundances in lung cancer samples	1663:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	4	11	theme	structural	622:631	arg1	information					633:643	useful structural information	615:643	useful structural information	615:643	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	5	12	theme	abundant	704:711	arg1	ions					713:716	two abundant ions	700:716	two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions	700:864	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	13	theme	residue	810:816	arg1	loss					785:788	the loss	781:788	the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions	781:864	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	14	theme	core	659:662	arg1	N-glycans					676:684	core fucosylated N-glycans	659:684	core fucosylated N-glycans	659:684	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	9	15	theme	cancer	1688:1693	arg1	samples					1695:1701	lung cancer samples	1683:1701	lung cancer samples	1683:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	5	16	theme	N-glycans	676:684	arg1	types					650:654	All types	646:654	All types of core fucosylated N-glycans	646:684	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	6	17	theme	unit	978:981	arg1	loss					956:959	the loss	952:959	the loss of the GlcNAcPHN unit (311 and 295 Da)	952:998	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	5	18	theme	C-glycosidic	741:752	arg1	cleavages					754:762	C-glycosidic cleavages	741:762	C-glycosidic cleavages	741:762	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	0	19	theme	Phenylhydrazine	17:31	arg1	Labeling					33:40	Phenylhydrazine Labeling	17:40	Phenylhydrazine Labeling	17:40	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.					
31141660	9	20	from	abundances	1669:1678	arg1	samples					1695:1701	lung cancer samples	1683:1701	lung cancer samples	1683:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	5	21	theme	consistent	718:727	arg1	ions					713:716	two abundant ions	700:716	two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions	700:864	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	3	22	from	utility	358:364	arg1	mode					507:510	the positive ion mode	490:510	the positive ion mode	490:510	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	0	23	theme	Structural	46:55	arg1	Studies					57:63	Structural Studies	46:63	Structural Studies of Fucosylated N-Glycans	46:88	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.					
31141660	3	24	theme	phenylhydrazine	369:383	arg1	labeling					391:398	phenylhydrazine (PHN) labeling	369:398	phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS)	369:485	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	3	25	theme	structural	404:413	arg1	studies					415:421	structural studies	404:421	structural studies of fucosylated N-glycans	404:446	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	5	26	theme	FucGlcNAcPHN	797:808	arg1	residue					810:816	the FucGlcNAcPHN residue	793:816	the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions	793:864	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	3	27	theme	tandem	451:456	arg1	MS					483:484	MS	483:484	MS	483:484	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	3	27	theme	tandem	451:456	arg1	spectrometry					469:480	tandem MALDI mass spectrometry	451:480	tandem MALDI mass spectrometry (MS)	451:485	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	8	28	from	information	1434:1444	arg1	topology					1453:1460	the topology	1449:1460	the topology of N-glycans, such as type of antennarity or identification of bisecting moiety	1449:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	8	29	gly	multifucosylated	1287:1302	arg1	glycans					1304:1310	multifucosylated glycans	1287:1310	multifucosylated glycans	1287:1310	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	3	30	theme	labeling	391:398	arg1	utility					358:364	utility	358:364	utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode	358:510	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	3	31	theme	N-glycans	438:446	arg1	studies					415:421	structural studies	404:421	structural studies of fucosylated N-glycans	404:446	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	7	32	theme	useful	1083:1088	arg1	cleavages					1090:1098	diagnostic useful cleavages	1072:1098	diagnostic useful cleavages	1072:1098	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	3	33	theme	PHN	386:388	arg1	labeling					391:398	phenylhydrazine (PHN) labeling	369:398	phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS)	369:485	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	6	34	from	N-glycans	899:907	arg1	types					873:877	These types	867:877	These types of fragment ions in N-glycans without a core fucose	867:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	0	35	theme	Fucosylated	68:78	arg1	N-Glycans					80:88	Fucosylated N-Glycans	68:88	Fucosylated N-Glycans	68:88	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.					
31141660	9	36	theme	practical	1547:1555	arg1	applicability					1557:1569	The practical applicability	1543:1569	The practical applicability of the approach	1543:1585	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	6	37	theme	chitobiose	1046:1055	arg1	residue					1057:1063	the chitobiose residue	1042:1063	the chitobiose residue	1042:1063	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	8	38	theme	many	1361:1364	arg1	residues					1373:1380	many fucose residues	1361:1380	many fucose residues	1361:1380	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	3	39	theme	mass	464:467	arg1	MS					483:484	MS	483:484	MS	483:484	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	3	39	theme	mass	464:467	arg1	spectrometry					469:480	tandem MALDI mass spectrometry	451:480	tandem MALDI mass spectrometry (MS)	451:485	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	8	40	theme	moiety	1535:1540	arg1	identification					1507:1520	identification	1507:1520	identification of bisecting moiety	1507:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	8	40	theme	moiety	1535:1540	arg1	type					1484:1487	type	1484:1487	type of antennarity	1484:1502	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	6	41	theme	ions	891:894	arg1	types					873:877	These types	867:877	These types of fragment ions in N-glycans without a core fucose	867:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	9	42	theme	approach	1578:1585	arg1	applicability					1557:1569	The practical applicability	1543:1569	The practical applicability of the approach	1543:1585	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	4	43	theme	MS/MS	578:582	arg1	pattern					598:604	the MS/MS fragmentation pattern	574:604	the MS/MS fragmentation pattern	574:604	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	6	44	from	ions	891:894	arg1	N-glycans					899:907	N-glycans	899:907	N-glycans without a core fucose	899:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	7	45	theme	rapid	1181:1185	arg1	recognition					1187:1197	rapid recognition	1181:1197	rapid recognition of antenna from core fucosylation in glycans detected with low abundances	1181:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	4	46	theme	specific	550:557	arg1	types					563:567	specific ion types	550:567	specific ion types	550:567	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	9	47	gly	multifucosylated	1622:1637	arg1	N-glycans					1639:1647	multifucosylated N-glycans	1622:1647	multifucosylated N-glycans detected with lower abundances in lung cancer samples	1622:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	4	48	theme	types	563:567	arg1	production					536:545	the production	532:545	the production of specific ion types	532:567	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	6	49	theme	residue	1057:1063	arg1	loss					1034:1037	the loss	1030:1037	the loss of the chitobiose residue	1030:1063	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	3	50	theme	ion	503:505	arg1	mode					507:510	the positive ion mode	490:510	the positive ion mode	490:510	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	1	51	theme	different	164:172	arg1	processes					198:206	different normal and pathological processes	164:206	different normal and pathological processes	164:206	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	9	52	theme	N-glycans	1639:1647	arg1	analysis					1610:1617	the analysis	1606:1617	the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples	1606:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	2	53	theme	fucose	304:309	arg1	position					311:318	the fucose position	300:318	the fucose position in one-step	300:330	Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step.					
31141660	8	54	theme	specific	1393:1400	arg1	antenna					1402:1408	the specific antenna	1389:1408	the specific antenna	1389:1408	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	4	55	theme	useful	615:620	arg1	information					633:643	useful structural information	615:643	useful structural information	615:643	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	9	56	theme	lung	1683:1686	arg1	samples					1695:1701	lung cancer samples	1683:1701	lung cancer samples	1683:1701	The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.					
31141660	5	57	with	consistent	718:727	arg1	B-					734:735	B-	734:735	B-	734:735	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	57	with	consistent	718:727	arg1	cleavages					754:762	C-glycosidic cleavages	741:762	C-glycosidic cleavages	741:762	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	58	theme	fucosylated	664:674	arg1	N-glycans					676:684	core fucosylated N-glycans	659:684	core fucosylated N-glycans	659:684	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	3	59	gly	fucosylated	426:436	arg1	N-glycans					438:446	fucosylated N-glycans	426:446	fucosylated N-glycans	426:446	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	8	60	theme	additional	1423:1432	arg1	information					1434:1444	additional information	1423:1444	additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety	1423:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	0	61	theme	N-Glycans	80:88	arg1	Studies					57:63	Structural Studies	46:63	Structural Studies of Fucosylated N-Glycans	46:88	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.					
31141660	5	62	theme	parent	854:859	arg1	ions					861:864	the parent ions	850:864	the parent ions	850:864	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	6	63	theme	GlcNAcPHN	968:976	arg1	Da					996:997	311 and 295 Da	984:997	311 and 295 Da	984:997	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	6	63	theme	GlcNAcPHN	968:976	arg1	unit					978:981	the GlcNAcPHN unit	964:981	the GlcNAcPHN unit (311 and 295 Da)	964:998	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	5	64	gly	fucosylated	664:674	arg1	N-glycans					676:684	core fucosylated N-glycans	659:684	core fucosylated N-glycans	659:684	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	2	65	from	position	311:318	arg1	one-step					323:330	one-step	323:330	one-step	323:330	Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step.					
31141660	8	66	theme	N-glycans	1465:1473	arg1	topology					1453:1460	the topology	1449:1460	the topology of N-glycans, such as type of antennarity or identification of bisecting moiety	1449:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	7	67	theme	significant	1119:1129	arg1	intensities					1131:1141	significant intensities	1119:1141	significant intensities	1119:1141	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	5	68	from	ions	861:864	arg1	Da					842:843	a mass 457 and 441 Da	823:843	Da	842:843	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	5	68	from	ions	861:864	arg1	mass					825:828	a mass 457 and 441 Da	823:843	mass	825:828	All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions.					
31141660	6	69	theme	fucose	1005:1010	arg1	cleavage					1012:1019	fucose cleavage	1005:1019	fucose cleavage	1005:1019	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	8	70	theme	multifucosylated	1287:1302	arg1	glycans					1304:1310	multifucosylated glycans	1287:1310	multifucosylated glycans	1287:1310	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	3	71	theme	fucosylated	426:436	arg1	N-glycans					438:446	fucosylated N-glycans	426:446	fucosylated N-glycans	426:446	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	1	72	from	site	138:141	arg1	glycans					146:152	glycans	146:152	glycans	146:152	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	7	73	theme	low	1258:1260	arg1	abundances					1262:1271	low abundances	1258:1271	low abundances	1258:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	0	74	theme	Labeling	33:40	arg1	Applicability					0:12	Applicability	0:12	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.	0:89	Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans.					
31141660	1	75	theme	common	109:114	arg1	Fucosylation					91:102	Fucosylation	91:102	Fucosylation	91:102	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	1	75	theme	common	109:114	arg1	modification					116:127	a common modification	107:127	a common modification	107:127	Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes.					
31141660	8	76	theme	antennarity	1492:1502	arg1	identification					1507:1520	identification	1507:1520	identification of bisecting moiety	1507:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	8	76	theme	antennarity	1492:1502	arg1	type					1484:1487	type	1484:1487	type of antennarity	1484:1502	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	3	77	theme	MALDI	458:462	arg1	MS					483:484	MS	483:484	MS	483:484	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	3	77	theme	MALDI	458:462	arg1	spectrometry					469:480	tandem MALDI mass spectrometry	451:480	tandem MALDI mass spectrometry (MS)	451:485	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
31141660	7	78	theme	diagnostic	1072:1081	arg1	cleavages					1090:1098	diagnostic useful cleavages	1072:1098	diagnostic useful cleavages	1072:1098	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	8	79	theme	labeling	1326:1333	arg1	type					1318:1321	this type	1313:1321	this type of labeling	1313:1333	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	7	80	gly	fucosylation	1220:1231	arg1	glycans					1236:1242	glycans	1236:1242	glycans detected with low abundances	1236:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	7	81	theme	core	1215:1218	arg1	fucosylation					1220:1231	core fucosylation	1215:1231	core fucosylation in glycans detected with low abundances	1215:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	8	82	theme	bisecting	1525:1533	arg1	moiety					1535:1540	bisecting moiety	1525:1540	bisecting moiety	1525:1540	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	2	83	theme	intensive	217:225	arg1	research					227:234	intensive research	217:234	intensive research	217:234	Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step.					
31141660	7	84	theme	antenna	1202:1208	arg1	recognition					1187:1197	rapid recognition	1181:1197	rapid recognition of antenna from core fucosylation in glycans detected with low abundances	1181:1271	Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances.					
31141660	4	85	theme	fragmentation	584:596	arg1	pattern					598:604	the MS/MS fragmentation pattern	574:604	the MS/MS fragmentation pattern	574:604	PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information.					
31141660	6	86	from	types	873:877	arg1	N-glycans					899:907	N-glycans	899:907	N-glycans without a core fucose	899:929	These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue.					
31141660	8	87	theme	fucose	1366:1371	arg1	residues					1373:1380	many fucose residues	1361:1380	many fucose residues	1361:1380	Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety.					
31141660	3	88	theme	positive	494:501	arg1	mode					507:510	the positive ion mode	490:510	the positive ion mode	490:510	In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode.					
30981779	3	0	theme	anion	337:341	arg1	chromatography					352:365	anion exchange chromatography	337:365	anion exchange chromatography	337:365	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	1	1	contain	has	150:152	arg2	history					161:167	a long history	154:167	a long history	154:167	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	1	1	contain	has	150:152	arg1	Frankincense					137:148	Frankincense	137:148	Frankincense	137:148	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	5	2	theme	small	701:705	arg1	amounts					707:713	small amounts	701:713	small amounts of rhamnose and glucose	701:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	2	theme	small	701:705	arg1	glucose					731:737	glucose	731:737	glucose	731:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	2	theme	small	701:705	arg1	rhamnose					718:725	rhamnose	718:725	rhamnose	718:725	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	6	3	theme	linkage	784:790	arg1	analysis					792:799	glycosyl linkage analysis	775:799	glycosyl linkage analysis	775:799	The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans.					
30981779	0	4	theme	fractions	83:91	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	0	4	theme	fractions	83:91	arg1	elucidation					21:31	structure elucidation	11:31	structure elucidation	11:31	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	0	4	theme	fractions	83:91	arg1	activity					56:63	immunostimulatory activity	38:63	immunostimulatory activity	38:63	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	10	5	theme	immunostimulatory	1168:1184	arg1	effect					1186:1191	an immunostimulatory effect	1165:1191	an immunostimulatory effect on RAW 264.7 murine macrophage cells	1165:1228	The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells.					
30981779	1	6	theme	religious	172:180	arg1	use					207:209	religious, cultural, and medicinal use	172:209	religious, cultural, and medicinal use	172:209	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	7	7	theme	protein	965:971	arg1	%					997:997	<1 wt%	992:997	<1 wt%	992:997	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	7	7	theme	protein	965:971	arg1	protein					965:971	protein	965:971	protein	965:971	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	7	7	theme	protein	965:971	arg1	amount					955:960	the amount	951:960	the amount of protein in the samples	951:986	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	0	8	theme	carterii	108:115	arg1	resin					130:134	Boswellia carterii frankincense resin	98:134	Boswellia carterii frankincense resin	98:134	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	8	9	theme	weight	1059:1064	arg1	compounds					1066:1074	high molecular weight compounds	1044:1074	high molecular weight compounds	1044:1074	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	8	10	theme	high	1044:1047	arg1	compounds					1066:1074	high molecular weight compounds	1044:1074	high molecular weight compounds	1044:1074	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	3	11	theme	Flow	392:395	arg1	column					408:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	10	12	theme	macrophage	1213:1222	arg1	cells					1224:1228	RAW 264.7 murine macrophage cells	1196:1228	RAW 264.7 murine macrophage cells	1196:1228	The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells.					
30981779	5	13	theme	rhamnose	718:725	arg1	amounts					707:713	small amounts	701:713	small amounts of rhamnose and glucose	701:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	13	theme	rhamnose	718:725	arg1	glucose					731:737	glucose	731:737	glucose	731:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	13	theme	rhamnose	718:725	arg1	rhamnose					718:725	rhamnose	718:725	rhamnose	718:725	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	6	14	theme	glycosyl	775:782	arg1	analysis					792:799	glycosyl linkage analysis	775:799	glycosyl linkage analysis	775:799	The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans.					
30981779	6	15	theme	monosaccharide	744:757	arg1	composition					759:769	The monosaccharide composition	740:769	The monosaccharide composition	740:769	The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans.					
30981779	10	16	theme	RAW	1196:1198	arg1	cells					1224:1228	RAW 264.7 murine macrophage cells	1196:1228	RAW 264.7 murine macrophage cells	1196:1228	The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells.					
30981779	5	17	theme	glucuronic	674:683	arg1	acid					685:688	glucuronic acid	674:688	glucuronic acid	674:688	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	8	18	with	consistent	1028:1037	arg1	compounds					1066:1074	high molecular weight compounds	1044:1074	high molecular weight compounds	1044:1074	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	12	19	theme	primary	1430:1436	arg1	splenocytes					1445:1455	primary murine splenocytes	1430:1455	primary murine splenocytes	1430:1455	The fractions were effective in proliferating primary murine splenocytes.					
30981779	0	20	theme	frankincense	117:128	arg1	resin					130:134	Boswellia carterii frankincense resin	98:134	Boswellia carterii frankincense resin	98:134	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	4	21	theme	size	522:525	arg1	chromatography					537:550	size exclusion chromatography	522:550	size exclusion chromatography	522:550	Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column.					
30981779	4	22	theme	exclusion	527:535	arg1	chromatography					537:550	size exclusion chromatography	522:550	size exclusion chromatography	522:550	Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column.					
30981779	5	23	theme	present	621:627	arg1	monosaccharides					605:619	the monosaccharides	601:619	the monosaccharides present	601:627	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	1	24	theme	cultural	183:190	arg1	use					207:209	religious, cultural, and medicinal use	172:209	religious, cultural, and medicinal use	172:209	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	3	25	theme	exchange	343:350	arg1	chromatography					352:365	anion exchange chromatography	337:365	anion exchange chromatography	337:365	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	11	26	theme	mediators	1318:1326	arg1	NO					1344:1345	NO	1344:1345	NO	1344:1345	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	26	theme	mediators	1318:1326	arg1	iNOS					1338:1341	iNOS	1338:1341	iNOS	1338:1341	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	26	theme	mediators	1318:1326	arg1	range					1292:1296	a range	1290:1296	a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6	1290:1362	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	26	theme	mediators	1318:1326	arg1	TNF-α					1348:1352	TNF-α	1348:1352	TNF-α	1348:1352	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	26	theme	mediators	1318:1326	arg1	IL-6					1359:1362	IL-6	1359:1362	IL-6	1359:1362	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	5	27	dep	showed	594:599	arg1	arabinose					659:667	arabinose	659:667	arabinose	659:667	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	27	dep	showed	594:599	arg1	galactose					648:656	galactose	648:656	galactose	648:656	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	27	dep	showed	594:599	arg1	acid					685:688	glucuronic acid	674:688	glucuronic acid	674:688	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	13	28	contain	have	1531:1534	arg1	polysaccharides					1488:1502	the polysaccharides	1484:1502	the polysaccharides isolated from frankincense	1484:1529	The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.					
30981779	13	28	contain	have	1531:1534	arg2	potential					1540:1548	the potential to be used as an immunological stimulant or nutraceutical	1536:1606	the potential to be used as an immunological stimulant or nutraceutical	1536:1606	The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.					
30981779	11	29	theme	RAW	1367:1369	arg1	cells					1377:1381	RAW 264.7 cells	1367:1381	RAW 264.7 cells	1367:1381	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	3	30	theme	16/10	397:401	arg1	column					408:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	11	31	theme	active	1240:1245	arg1	FA2					1274:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	3	32	theme	Fast	387:390	arg1	column					408:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	6	33	theme	type	844:847	arg1	arabinogalactans					852:867	the type II arabinogalactans	840:867	the type II arabinogalactans	840:867	The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans.					
30981779	1	34	theme	medicinal	197:205	arg1	use					207:209	religious, cultural, and medicinal use	172:209	religious, cultural, and medicinal use	172:209	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	3	35	theme	FPLC	403:406	arg1	column					408:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	7	36	theme	Fourier-transform	870:886	arg1	spectroscopy					897:908	Fourier-transform infrared spectroscopy	870:908	Fourier-transform infrared spectroscopy	870:908	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	4	37	theme	immunomodulatory	470:485	arg1	fractions					487:495	the three most active immunomodulatory fractions	448:495	the three most active immunomodulatory fractions	448:495	Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column.					
30981779	11	38	theme	pro-inflammatory	1301:1316	arg1	NO					1344:1345	NO	1344:1345	NO	1344:1345	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	38	theme	pro-inflammatory	1301:1316	arg1	iNOS					1338:1341	iNOS	1338:1341	iNOS	1338:1341	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	38	theme	pro-inflammatory	1301:1316	arg1	mediators					1318:1326	pro-inflammatory mediators	1301:1326	pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6	1301:1362	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	38	theme	pro-inflammatory	1301:1316	arg1	TNF-α					1348:1352	TNF-α	1348:1352	TNF-α	1348:1352	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	38	theme	pro-inflammatory	1301:1316	arg1	IL-6					1359:1362	IL-6	1359:1362	IL-6	1359:1362	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	7	39	theme	bicinchoninic	914:926	arg1	assay					933:937	bicinchoninic acid assay	914:937	bicinchoninic acid assay	914:937	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	7	40	theme	infrared	888:895	arg1	spectroscopy					897:908	Fourier-transform infrared spectroscopy	870:908	Fourier-transform infrared spectroscopy	870:908	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	4	41	theme	active	463:468	arg1	fractions					487:495	the three most active immunomodulatory fractions	448:495	the three most active immunomodulatory fractions	448:495	Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column.					
30981779	7	42	theme	acid	928:931	arg1	assay					933:937	bicinchoninic acid assay	914:937	bicinchoninic acid assay	914:937	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	12	43	theme	murine	1438:1443	arg1	splenocytes					1445:1455	primary murine splenocytes	1430:1455	primary murine splenocytes	1430:1455	The fractions were effective in proliferating primary murine splenocytes.					
30981779	8	44	theme	1H	1016:1017	arg1	NMR					1019:1021	One-dimensional 1H NMR	1000:1021	One-dimensional 1H NMR	1000:1021	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	3	45	theme	DEAE-Sepharose	372:385	arg1	column					408:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	a DEAE-Sepharose Fast Flow 16/10 FPLC column	370:413	The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column.					
30981779	4	46	theme	Superdex-200	557:568	arg1	column					570:575	a Superdex-200 column	555:575	a Superdex-200 column	555:575	Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column.					
30981779	8	47	theme	One-dimensional	1000:1014	arg1	NMR					1019:1021	One-dimensional 1H NMR	1000:1021	One-dimensional 1H NMR	1000:1021	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	0	48	theme	immunostimulatory	38:54	arg1	activity					56:63	immunostimulatory activity	38:63	immunostimulatory activity	38:63	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	13	49	theme	immunological	1567:1579	arg1	stimulant					1581:1589	an immunological stimulant	1564:1589	an immunological stimulant	1564:1589	The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.					
30981779	5	50	theme	glucose	731:737	arg1	amounts					707:713	small amounts	701:713	small amounts of rhamnose and glucose	701:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	50	theme	glucose	731:737	arg1	glucose					731:737	glucose	731:737	glucose	731:737	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	5	50	theme	glucose	731:737	arg1	rhamnose					718:725	rhamnose	718:725	rhamnose	718:725	The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose.					
30981779	0	51	from	resin	130:134	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	0	51	from	resin	130:134	arg1	elucidation					21:31	structure elucidation	11:31	structure elucidation	11:31	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	0	51	from	resin	130:134	arg1	activity					56:63	immunostimulatory activity	38:63	immunostimulatory activity	38:63	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	11	52	theme	immunostimulatory	1247:1263	arg1	FA2					1274:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	11	53	theme	fraction	1265:1272	arg1	FA2					1274:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2	1231:1276	The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells.					
30981779	10	54	theme	murine	1206:1211	arg1	cells					1224:1228	RAW 264.7 murine macrophage cells	1196:1228	RAW 264.7 murine macrophage cells	1196:1228	The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells.					
30981779	9	55	theme	β	1119:1119	arg1	conformation					1121:1132	the β conformation	1115:1132	the β conformation	1115:1132	The monosaccharides were primarily in the β conformation.					
30981779	13	56	attach	isolated	1504:1511	arg2	polysaccharides					1488:1502	the polysaccharides	1484:1502	the polysaccharides isolated from frankincense	1484:1529	The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.					
30981779	13	56	attach	isolated	1504:1511	arg1	frankincense					1518:1529	frankincense	1518:1529	frankincense	1518:1529	The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.					
30981779	0	57	theme	polysaccharide	68:81	arg1	fractions					83:91	polysaccharide fractions	68:91	polysaccharide fractions	68:91	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	7	58	from	amount	955:960	arg1	samples					980:986	the samples	976:986	the samples	976:986	Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%.					
30981779	10	59	from	effect	1186:1191	arg1	cells					1224:1228	RAW 264.7 murine macrophage cells	1196:1228	RAW 264.7 murine macrophage cells	1196:1228	The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells.					
30981779	1	60	theme	long	156:159	arg1	history					161:167	a long history	154:167	a long history	154:167	Frankincense has a long history in religious, cultural, and medicinal use.					
30981779	0	61	theme	structure	11:19	arg1	elucidation					21:31	structure elucidation	11:31	structure elucidation	11:31	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30981779	8	62	theme	molecular	1049:1057	arg1	compounds					1066:1074	high molecular weight compounds	1044:1074	high molecular weight compounds	1044:1074	One-dimensional 1H NMR were consistent with high molecular weight compounds.					
30981779	6	63	dep	belong	830:835	arg1	revealed					801:808	revealed	801:808	revealed	801:808	The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans.					
30981779	0	64	theme	Boswellia	98:106	arg1	resin					130:134	Boswellia carterii frankincense resin	98:134	Boswellia carterii frankincense resin	98:134	Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin.					
30557003	5	0	theme	tag	932:934	arg1	N-glycan					944:951	the fluorous tag labeled N-glycan	919:951	the fluorous tag labeled N-glycan	919:951	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	4	1	theme	tag	614:616	arg1	labeling					618:625	isotopically fluorous tag labeling	592:625	isotopically fluorous tag labeling	592:625	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	3	2	theme	N-glycome	342:350	arg1	quantitation					326:337	the quantitation	322:337	the quantitation of N-glycome with high accuracy and sensitivity	322:385	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	4	3	theme	oxime	847:851	arg1	reaction					859:866	the oxime click reaction	843:866	the oxime click reaction	843:866	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	3	4	from	substances	522:531	arg1	suppression					499:509	suppression	499:509	suppression from other substances	499:531	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	4	5	dep	tags	808:811	arg1	PFBHA					814:818	PFBHA	814:818	PFBHA	814:818	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	5	dep	tags	808:811	arg1	d2					831:832	PFBHA- d2	824:832	PFBHA- d2	824:832	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	5	dep	tags	808:811	arg1	tags					808:811	heavy aminoxy-functionalized fluorous tags	770:811	heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2)	770:833	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	8	6	theme	hepatocellular	1624:1637	arg1	HCC					1650:1652	HCC	1650:1652	HCC	1650:1652	Finally, this strategy was successfully applied to analyze the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC).					
30557003	8	6	theme	hepatocellular	1624:1637	arg1	carcinoma					1639:1647	hepatocellular carcinoma	1624:1647	hepatocellular carcinoma (HCC)	1624:1653	Finally, this strategy was successfully applied to analyze the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC).					
30557003	4	7	theme	fluorous	605:612	arg1	labeling					618:625	isotopically fluorous tag labeling	592:625	isotopically fluorous tag labeling	592:625	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	9	8	theme	sialic	1709:1714	arg1	acid					1716:1719	sialic acid	1709:1719	sialic acid	1709:1719	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	5	9	theme	quantitative	1031:1042	arg1	analysis					1044:1051	the following quantitative analysis	1017:1051	the following quantitative analysis by mass spectrometry	1017:1072	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	5	10	theme	mass	1056:1059	arg1	spectrometry					1061:1072	mass spectrometry	1056:1072	mass spectrometry	1056:1072	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	6	11	theme	variation	1227:1235	arg1	coefficient					1212:1222	coefficient	1212:1222	coefficient of variation (CV)	1212:1240	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	12	theme	accurate	1172:1179	arg1	quantitation					1257:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation	1172:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans	1172:1281	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	7	13	theme	6-antenna	1475:1483	arg1	composition					1456:1466	the composition	1452:1466	the composition of the 6-antenna	1452:1483	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	0	14	theme	N-Glycome	94:102	arg1	Analysis					82:89	Quantitative Analysis	69:89	Quantitative Analysis of N-Glycome by Mass Spectrometry	69:123	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	7	15	theme	fluorous	1398:1405	arg1	labeling					1412:1419	fluorous tags labeling	1398:1419	fluorous tags labeling	1398:1419	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	7	16	theme	MS	1380:1381	arg1	analysis					1383:1390	tandem MS analysis	1373:1390	tandem MS analysis	1373:1390	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	6	17	theme	glycan	1140:1145	arg1	protein					1150:1156	glycan to protein	1140:1156	glycan to protein	1140:1156	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	5	18	theme	fluorous	886:893	arg1	extraction					907:916	the fluorous solid-phase extraction	882:916	the fluorous solid-phase extraction	882:916	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	9	19	gly	fucosylation	1731:1742	arg1	compositions					1673:1684	Fifteen N-glycan compositions	1656:1684	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation	1656:1742	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	6	20	dep	accurate	1172:1179	arg1	>					1186:1186	R2 > 0.99	1183:1191	R2 > 0.99	1183:1191	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	20	dep	accurate	1172:1179	arg1	coefficient					1212:1222	coefficient	1212:1222	coefficient of variation (CV)	1212:1240	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	8	21	from	changes	1585:1591	arg1	serum					1602:1606	human serum	1596:1606	human serum associated with hepatocellular carcinoma (HCC)	1596:1653	Finally, this strategy was successfully applied to analyze the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC).					
30557003	1	22	theme	N-glycan	185:192	arg1	expression					171:180	abnormal expression	162:180	abnormal expression of N-glycan in diseases	162:204	Quantitative N-glycomics can reveal abnormal expression of N-glycan in diseases.					
30557003	0	23	theme	Mass	107:110	arg1	Spectrometry					112:123	Mass Spectrometry	107:123	Mass Spectrometry	107:123	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	5	24	theme	labeled	936:942	arg1	N-glycan					944:951	the fluorous tag labeled N-glycan	919:951	the fluorous tag labeled N-glycan	919:951	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	9	25	with	compositions	1673:1684	arg1	GlcNAc					1701:1706	bisecting GlcNAc	1691:1706	bisecting GlcNAc	1691:1706	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	9	25	with	compositions	1673:1684	arg1	acid					1716:1719	sialic acid	1709:1719	sialic acid	1709:1719	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	9	25	with	compositions	1673:1684	arg1	fucosylation					1731:1742	core fucosylation	1726:1742	core fucosylation	1726:1742	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	3	26	with	N-glycans	428:436	arg1	tags					452:455	isotopic tags	443:455	isotopic tags	443:455	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	5	27	theme	solid-phase	895:905	arg1	extraction					907:916	the fluorous solid-phase extraction	882:916	the fluorous solid-phase extraction	882:916	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	9	28	theme	core	1726:1729	arg1	fucosylation					1731:1742	core fucosylation	1726:1742	core fucosylation	1726:1742	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	4	29	theme	heavy	770:774	arg1	PFBHA					814:818	PFBHA	814:818	PFBHA	814:818	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	29	theme	heavy	770:774	arg1	d2					831:832	PFBHA- d2	824:832	PFBHA- d2	824:832	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	29	theme	heavy	770:774	arg1	tags					808:811	heavy aminoxy-functionalized fluorous tags	770:811	heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2)	770:833	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	7	30	theme	tags	1407:1410	arg1	labeling					1412:1419	fluorous tags labeling	1398:1419	fluorous tags labeling	1398:1419	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	0	31	theme	Integrated	0:9	arg1	Pipeline					11:18	Integrated Pipeline	0:18	Integrated Pipeline of Isotopic Labeling	0:39	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	7	32	theme	tandem	1373:1378	arg1	analysis					1383:1390	tandem MS analysis	1373:1390	tandem MS analysis	1373:1390	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	6	33	theme	<	1243:1243	arg1	%					1247:1247	< 25%	1243:1247	< 25%	1243:1247	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	34	theme	R2	1183:1184	arg1	>					1186:1186	R2 > 0.99	1183:1191	R2 > 0.99	1183:1191	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	3	35	with	quantitation	326:337	arg1	sensitivity					375:385	sensitivity	375:385	sensitivity	375:385	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	3	35	with	quantitation	326:337	arg1	accuracy					362:369	high accuracy	357:369	high accuracy	357:369	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	0	36	theme	Isotopic	23:30	arg1	Labeling					32:39	Isotopic Labeling	23:39	Isotopic Labeling	23:39	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	6	37	theme	magnitude	1302:1310	arg1	orders					1292:1297	2 orders	1290:1297	2 orders of magnitude	1290:1310	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	4	38	theme	fluorous	799:806	arg1	PFBHA					814:818	PFBHA	814:818	PFBHA	814:818	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	38	theme	fluorous	799:806	arg1	d2					831:832	PFBHA- d2	824:832	PFBHA- d2	824:832	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	38	theme	fluorous	799:806	arg1	tags					808:811	heavy aminoxy-functionalized fluorous tags	770:811	heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2)	770:833	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	1	39	from	expression	171:180	arg1	diseases					197:204	diseases	197:204	diseases	197:204	Quantitative N-glycomics can reveal abnormal expression of N-glycan in diseases.					
30557003	6	40	theme	selective	1101:1109	arg1	purification					1111:1122	selective purification	1101:1122	selective purification (molar ratio of glycan to protein at 1:100)	1101:1166	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	41	dep	purification	1111:1122	arg1	ratio					1131:1135	molar ratio	1125:1135	molar ratio of glycan to protein at 1:100	1125:1165	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	42	theme	reproducible	1198:1209	arg1	quantitation					1257:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation	1172:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans	1172:1281	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	4	43	theme	aminoxy-functionalized	776:797	arg1	PFBHA					814:818	PFBHA	814:818	PFBHA	814:818	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	43	theme	aminoxy-functionalized	776:797	arg1	d2					831:832	PFBHA- d2	824:832	PFBHA- d2	824:832	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	43	theme	aminoxy-functionalized	776:797	arg1	tags					808:811	heavy aminoxy-functionalized fluorous tags	770:811	heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2)	770:833	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	7	44	theme	diagnostic	1323:1332	arg1	[D-221					1346:1351	[D-221	1346:1351	[D-221	1346:1351	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	7	44	theme	diagnostic	1323:1332	arg1	D					1340:1340	D	1340:1340	D	1340:1340	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	7	44	theme	diagnostic	1323:1332	arg1	ions					1334:1337	diagnostic ions	1323:1337	diagnostic ions (D and [D-221])	1323:1353	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	1	45	theme	Quantitative	126:137	arg1	N-glycomics					139:149	Quantitative N-glycomics	126:149	Quantitative N-glycomics	126:149	Quantitative N-glycomics can reveal abnormal expression of N-glycan in diseases.					
30557003	2	46	theme	-based	238:243	arg1	analysis					268:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	3	47	theme	isotopic	443:450	arg1	tags					452:455	isotopic tags	443:455	isotopic tags	443:455	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	0	48	theme	Labeling	32:39	arg1	Pipeline					11:18	Integrated Pipeline	0:18	Integrated Pipeline of Isotopic Labeling	0:39	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	7	49	used	used	1437:1440	arg2	analysis					1383:1390	tandem MS analysis	1373:1390	tandem MS analysis	1373:1390	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	6	50	from	1:100	1161:1165	arg1	ratio					1131:1135	molar ratio	1125:1135	molar ratio of glycan to protein at 1:100	1125:1165	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	51	theme	new	1080:1082	arg1	approach					1084:1091	This new approach	1075:1091	This new approach	1075:1091	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	9	52	theme	HCC	1778:1780	arg1	serum					1782:1786	HCC serum	1778:1786	HCC serum	1778:1786	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	9	53	theme	significant	1751:1761	arg1	differences					1763:1773	significant differences	1751:1773	significant differences in HCC serum	1751:1786	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	9	54	theme	bisecting	1691:1699	arg1	GlcNAc					1701:1706	bisecting GlcNAc	1691:1706	bisecting GlcNAc	1691:1706	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	6	55	dep	protein	1150:1156	arg1	to					1147:1148	to	1147:1148	to	1147:1148	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	56	dep	coefficient	1212:1222	arg1	%					1247:1247	< 25%	1243:1247	< 25%	1243:1247	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	56	dep	coefficient	1212:1222	arg1	n					1250:1250	n = 6	1250:1254	n = 6	1250:1254	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	4	57	theme	integrated	558:567	arg1	pipeline					569:576	an integrated pipeline	555:576	an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction	555:866	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	6	58	theme	N-glycans	1273:1281	arg1	quantitation					1257:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation	1172:1268	accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans	1172:1281	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	58	theme	N-glycans	1273:1281	arg1	purification					1111:1122	selective purification	1101:1122	selective purification (molar ratio of glycan to protein at 1:100)	1101:1166	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	2	59	theme	spectrometry	221:232	arg1	analysis					268:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	4	60	theme	click	853:857	arg1	reaction					859:866	the oxime click reaction	843:866	the oxime click reaction	843:866	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	3	61	theme	other	516:520	arg1	substances					522:531	other substances	516:531	other substances	516:531	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	9	62	theme	N-glycan	1664:1671	arg1	compositions					1673:1684	Fifteen N-glycan compositions	1656:1684	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation	1656:1742	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	2	63	theme	mass	216:219	arg1	MS					235:236	MS	235:236	MS	235:236	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	2	63	theme	mass	216:219	arg1	spectrometry					221:232	mass spectrometry	216:232	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	5	64	theme	following	1021:1029	arg1	analysis					1044:1051	the following quantitative analysis	1017:1051	the following quantitative analysis by mass spectrometry	1017:1072	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	8	65	theme	N-glycan	1576:1583	arg1	changes					1585:1591	the N-glycan changes	1572:1591	the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC)	1572:1653	Finally, this strategy was successfully applied to analyze the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC).					
30557003	4	66	theme	solid-phase	640:650	arg1	extraction					652:661	fluorous solid-phase extraction	631:661	fluorous solid-phase extraction	631:661	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	7	67	dep	ions	1334:1337	arg1	[D-221					1346:1351	[D-221	1346:1351	[D-221	1346:1351	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	7	67	dep	ions	1334:1337	arg1	D					1340:1340	D	1340:1340	D	1340:1340	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	7	67	dep	ions	1334:1337	arg1	ions					1334:1337	diagnostic ions	1323:1337	diagnostic ions (D and [D-221])	1323:1353	Uniquely, diagnostic ions (D and [D-221]) were generated in tandem MS analysis after fluorous tags labeling, which could be used to deduce the composition of the 6-antenna and to distinguish isomers.					
30557003	4	68	theme	PFBHA-	824:829	arg1	d2					831:832	PFBHA- d2	824:832	PFBHA- d2	824:832	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	4	68	theme	PFBHA-	824:829	arg1	tags					808:811	heavy aminoxy-functionalized fluorous tags	770:811	heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2)	770:833	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	9	69	from	differences	1763:1773	arg1	serum					1782:1786	HCC serum	1778:1786	HCC serum	1778:1786	Fifteen N-glycan compositions with bisecting GlcNAc, sialic acid, and core fucosylation showed significant differences in HCC serum.					
30557003	5	70	theme	fluorous	923:930	arg1	N-glycan					944:951	the fluorous tag labeled N-glycan	919:951	the fluorous tag labeled N-glycan	919:951	Then through the fluorous solid-phase extraction, the fluorous tag labeled N-glycan could be purified from contaminants like salts and proteins for the following quantitative analysis by mass spectrometry.					
30557003	8	71	theme	human	1596:1600	arg1	serum					1602:1606	human serum	1596:1606	human serum associated with hepatocellular carcinoma (HCC)	1596:1653	Finally, this strategy was successfully applied to analyze the N-glycan changes in human serum associated with hepatocellular carcinoma (HCC).					
30557003	4	72	theme	fluorous	631:638	arg1	extraction					652:661	fluorous solid-phase extraction	631:661	fluorous solid-phase extraction	631:661	Herein, we developed an integrated pipeline that combines isotopically fluorous tag labeling and fluorous solid-phase extraction to quantitatively analyze the N-glycome by MS. In this strategy, the N-glycans were labeled with light and heavy aminoxy-functionalized fluorous tags (PFBHA and PFBHA- d2) through the oxime click reaction.					
30557003	0	73	theme	Quantitative	69:80	arg1	Analysis					82:89	Quantitative Analysis	69:89	Quantitative Analysis of N-Glycome by Mass Spectrometry	69:123	Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.					
30557003	2	74	theme	quantitative	255:266	arg1	analysis					268:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	6	75	theme	protein	1150:1156	arg1	ratio					1131:1135	molar ratio	1125:1135	molar ratio of glycan to protein at 1:100	1125:1165	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	6	76	theme	molar	1125:1129	arg1	ratio					1131:1135	molar ratio	1125:1135	molar ratio of glycan to protein at 1:100	1125:1165	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
30557003	3	77	theme	high	357:360	arg1	accuracy					362:369	high accuracy	357:369	high accuracy	357:369	To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances.					
30557003	2	78	theme	N-glycome	245:253	arg1	analysis					268:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	mass spectrometry (MS)-based N-glycome quantitative analysis	216:275	However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging.					
30557003	1	79	theme	abnormal	162:169	arg1	expression					171:180	abnormal expression	162:180	abnormal expression of N-glycan in diseases	162:204	Quantitative N-glycomics can reveal abnormal expression of N-glycan in diseases.					
30557003	6	80	theme	=	1252:1252	arg1	n					1250:1250	n = 6	1250:1254	n = 6	1250:1254	This new approach enables selective purification (molar ratio of glycan to protein at 1:100) and accurate ( R2 > 0.99) and reproducible (coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.					
31364262	7	0	dep	Deoxyhexose	1522:1532	arg1	2					1553:1553	2	1553:1553	2	1553:1553	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	2	1	theme	ovarian	424:430	arg1	development					439:449	ovarian cancer development	424:449	ovarian cancer development	424:449	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	1	2	theme	gynecological	171:183	arg1	malignancies					185:196	the most fatal gynecological malignancies	156:196	the most fatal gynecological malignancies in adult women	156:211	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	4	3	theme	graphitized	786:796	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	3	theme	graphitized	786:796	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	6	4	theme	spatial	1149:1155	arg1	distribution					1157:1168	The spatial distribution	1145:1168	The spatial distribution of oligomannose	1145:1184	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	6	5	theme	early-stage	1333:1343	arg1	patients					1345:1352	early-stage patients	1333:1352	early-stage patients	1333:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	3	6	theme	cancer	645:650	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	2	7	theme	protein	228:234	arg1	N-glycosylation					236:250	protein N-glycosylation	228:250	protein N-glycosylation	228:250	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	7	8	theme	Hex	1505:1507	arg1	structure					1493:1501	the complex neutral structure	1473:1501	the complex neutral structure	1473:1501	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	8	theme	Hex	1505:1507	arg1	Deoxyhexose					1522:1532	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	3	9	theme	early-	673:678	arg1	patients					695:702	early- and late-stage patients	673:702	early- and late-stage patients	673:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	1	10	theme	ovarian	131:137	arg1	cancer					139:144	Epithelial ovarian cancer	120:144	Epithelial ovarian cancer	120:144	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	3	11	theme	formalin-fixed	604:617	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	1	12	from	malignancies	185:196	arg1	women					207:211	adult women	201:211	adult women	201:211	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	6	13	theme	bisecting	1204:1212	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	7	14	theme	neutral	1485:1491	arg1	structure					1493:1501	the complex neutral structure	1473:1501	the complex neutral structure	1473:1501	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	14	theme	neutral	1485:1491	arg1	Deoxyhexose					1522:1532	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	14	theme	neutral	1485:1491	arg1	Man					1539:1541	(Man)3	1538:1543	(Man)3 (GlcNAc)	1538:1552	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	4	15	theme	chromatography-electrospray	812:838	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	15	theme	chromatography-electrospray	812:838	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	2	16	from	patterns	287:294	arg1	microenvironment					324:339	the ovarian cancer tumor microenvironment	299:339	the ovarian cancer tumor microenvironment	299:339	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	0	17	theme	Serous	57:62	arg1	Tissue					79:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	0	18	theme	Spectrometry	11:22	arg1	Imaging					24:30	MALDI Mass Spectrometry Imaging	0:30	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue	0:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	5	19	theme	structural	1055:1064	arg1	N-glycans					1034:1042	42 N-glycans	1031:1042	42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS	1031:1142	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	5	19	theme	structural	1055:1064	arg1	isomers					1084:1090	including structural and compositional isomers	1045:1090	including structural and compositional isomers	1045:1090	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	6	20	theme	neutral	1195:1201	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	4	21	theme	mass	858:861	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	21	theme	mass	858:861	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	0	22	theme	Cancer	72:77	arg1	Tissue					79:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	5	23	theme	compositional	1070:1082	arg1	N-glycans					1034:1042	42 N-glycans	1031:1042	42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS	1031:1142	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	5	23	theme	compositional	1070:1082	arg1	isomers					1084:1090	including structural and compositional isomers	1045:1090	including structural and compositional isomers	1045:1090	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	4	24	theme	high-resolution	917:931	arg1	images					933:938	high-resolution images	917:938	high-resolution images obtained from MALDI-MSI	917:962	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	7	25	theme	complex	1477:1483	arg1	structure					1493:1501	the complex neutral structure	1473:1501	the complex neutral structure	1473:1501	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	25	theme	complex	1477:1483	arg1	Deoxyhexose					1522:1532	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	25	theme	complex	1477:1483	arg1	Man					1539:1541	(Man)3	1538:1543	(Man)3 (GlcNAc)	1538:1552	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	3	26	theme	laser	484:488	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	26	theme	laser	484:488	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	0	27	theme	MALDI	0:4	arg1	Spectrometry					11:22	MALDI Mass Spectrometry	0:22	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue	0:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	7	28	theme	N-glycan	1365:1372	arg1	markers					1385:1391	Potential N-glycan diagnostic markers	1355:1391	Potential N-glycan diagnostic markers that emerge	1355:1403	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	6	29	theme	N-glycan	1230:1237	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	8	30	theme	tissue	1612:1617	arg1	microarray					1619:1628	a tissue microarray	1610:1628	a tissue microarray of early- and late-stage patients	1610:1662	The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients.					
31364262	5	31	dep	N-glycans	1034:1042	arg1	identified					1093:1102	identified	1093:1102	identified	1093:1102	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	5	31	dep	N-glycans	1034:1042	arg1	characterized					1121:1133	characterized	1121:1133	structurally characterized by LC-MS	1108:1142	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	5	32	theme	Spatial	965:971	arg1	distribution					973:984	Spatial distribution	965:984	Spatial distribution of 14 N-glycans	965:1000	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	6	33	theme	patients	1312:1319	arg1	regions					1275:1281	the tumor regions	1265:1281	the tumor regions of late-stage ovarian cancer patients relative to early-stage patients	1265:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	2	34	theme	spatial	352:358	arg1	information					360:370	spatial information	352:370	spatial information	352:370	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	5	35	theme	N-glycans	992:1000	arg1	distribution					973:984	Spatial distribution	965:984	Spatial distribution of 14 N-glycans	965:1000	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	6	36	theme	ovarian	1297:1303	arg1	patients					1312:1319	late-stage ovarian cancer patients	1286:1319	late-stage ovarian cancer patients relative to early-stage patients	1286:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	8	37	theme	patients	1655:1662	arg1	microarray					1619:1628	a tissue microarray	1610:1628	a tissue microarray of early- and late-stage patients	1610:1662	The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients.					
31364262	4	38	theme	Tumor-specific	705:718	arg1	N-glycans					720:728	Tumor-specific N-glycans	705:728	Tumor-specific N-glycans	705:728	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	7	39	theme	oligomannose	1417:1428	arg1	structure					1430:1438	the oligomannose structure	1413:1438	the oligomannose structure	1413:1438	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	39	theme	oligomannose	1417:1428	arg1	Hex					1442:1444	(Hex)6	1441:1446	(Hex)6	1441:1446	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	39	theme	oligomannose	1417:1428	arg1	Man					1451:1453	(Man)3	1450:1455	(Man)3 (GlcNAc)	1450:1464	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	2	40	theme	tumor-specific	385:398	arg1	alterations					409:419	tumor-specific N-glycan alterations	385:419	tumor-specific N-glycan alterations in ovarian cancer development and progression	385:465	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	6	41	theme	tumor	1269:1273	arg1	regions					1275:1281	the tumor regions	1265:1281	the tumor regions of late-stage ovarian cancer patients relative to early-stage patients	1265:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	2	42	theme	ovarian	303:309	arg1	microenvironment					324:339	the ovarian cancer tumor microenvironment	299:339	the ovarian cancer tumor microenvironment	299:339	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	1	43	theme	fatal	165:169	arg1	malignancies					185:196	the most fatal gynecological malignancies	156:196	the most fatal gynecological malignancies in adult women	156:211	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	3	44	theme	tissue	652:657	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	1	45	theme	malignancies	185:196	arg1	malignancies					185:196	the most fatal gynecological malignancies	156:196	the most fatal gynecological malignancies in adult women	156:211	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	1	45	theme	malignancies	185:196	arg1	one					149:151	one	149:151	one	149:151	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	2	46	from	alterations	409:419	arg1	progression					455:465	progression	455:465	progression	455:465	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	2	46	from	alterations	409:419	arg1	development					439:449	ovarian cancer development	424:449	ovarian cancer development	424:449	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	4	47	theme	porous	779:784	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	47	theme	porous	779:784	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	3	48	theme	ovarian	637:643	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	1	49	theme	adult	201:205	arg1	women					207:211	adult women	201:211	adult women	201:211	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	2	50	theme	tumor	318:322	arg1	microenvironment					324:339	the ovarian cancer tumor microenvironment	299:339	the ovarian cancer tumor microenvironment	299:339	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	1	51	theme	Epithelial	120:129	arg1	cancer					139:144	Epithelial ovarian cancer	120:144	Epithelial ovarian cancer	120:144	Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women.					
31364262	2	52	theme	aberrant	278:285	arg1	patterns					287:294	aberrant patterns	278:294	aberrant patterns in the ovarian cancer tumor microenvironment	278:339	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	3	53	dep	formalin-fixed	604:617	arg1	paraffin-embedded					619:635	paraffin-embedded	619:635	paraffin-embedded	619:635	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	7	54	theme	HexNAc	1512:1517	arg1	structure					1493:1501	the complex neutral structure	1473:1501	the complex neutral structure	1473:1501	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	7	54	theme	HexNAc	1512:1517	arg1	Deoxyhexose					1522:1532	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	(Hex)2 (HexNAc)2 (Deoxyhexose)1	1504:1534	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	0	55	theme	Ovarian	64:70	arg1	Tissue					79:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	5	56	theme	including	1045:1053	arg1	N-glycans					1034:1042	42 N-glycans	1031:1042	42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS	1031:1142	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	5	56	theme	including	1045:1053	arg1	isomers					1084:1090	including structural and compositional isomers	1045:1090	including structural and compositional isomers	1045:1090	Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS.					
31364262	4	57	theme	carbon-liquid	798:810	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	57	theme	carbon-liquid	798:810	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	3	58	from	distribution	588:599	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	0	59	theme	Tissue	79:84	arg1	Imaging					24:30	MALDI Mass Spectrometry Imaging	0:30	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue	0:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	6	60	theme	complex	1187:1193	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	4	61	theme	ionization-tandem	840:856	arg1	PGC-LC-ESI-MS/MS					877:892	PGC-LC-ESI-MS/MS	877:892	PGC-LC-ESI-MS/MS	877:892	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	4	61	theme	ionization-tandem	840:856	arg1	spectrometry					863:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry	779:874	porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS)	779:893	Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI.					
31364262	0	62	theme	Stage-Specific	94:107	arg1	N-Glycans					109:117	Stage-Specific N-Glycans	94:117	Stage-Specific N-Glycans	94:117	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	2	63	theme	cancer	311:316	arg1	microenvironment					324:339	the ovarian cancer tumor microenvironment	299:339	the ovarian cancer tumor microenvironment	299:339	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	6	64	theme	oligomannose	1173:1184	arg1	distribution					1157:1168	The spatial distribution	1145:1168	The spatial distribution of oligomannose	1145:1184	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	8	65	theme	markers	1583:1589	arg1	distribution					1561:1572	The distribution	1557:1572	The distribution of these markers	1557:1589	The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients.					
31364262	3	66	from	patients	695:702	arg1	sections					659:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections	604:666	formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	604:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	2	67	from	studies	217:223	arg1	N-glycosylation					236:250	protein N-glycosylation	228:250	protein N-glycosylation	228:250	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	3	68	theme	MALDI	513:517	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	68	theme	MALDI	513:517	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	0	69	theme	Mass	6:9	arg1	Spectrometry					11:22	MALDI Mass Spectrometry	0:22	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue	0:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	7	70	dep	Hex	1442:1444	arg1	2					1465:1465	2	1465:1465	2	1465:1465	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	3	71	theme	desorption/ionization	490:510	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	71	theme	desorption/ionization	490:510	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	7	72	theme	Potential	1355:1363	arg1	markers					1385:1391	Potential N-glycan diagnostic markers	1355:1391	Potential N-glycan diagnostic markers that emerge	1355:1403	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
31364262	3	73	theme	imaging	538:544	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	73	theme	imaging	538:544	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	0	74	theme	Early-	35:40	arg1	Tissue					79:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	6	75	theme	sialylated	1219:1228	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	3	76	theme	matrix-assisted	468:482	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	76	theme	matrix-assisted	468:482	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	8	77	theme	early-	1633:1638	arg1	patients					1655:1662	early- and late-stage patients	1633:1662	early- and late-stage patients	1633:1662	The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients.					
31364262	6	78	gly	sialylated	1219:1228	arg1	families					1239:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	complex neutral, bisecting, and sialylated N-glycan families	1187:1246	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	3	79	theme	mass	520:523	arg1	spectrometry					525:536	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging	468:544	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI)	468:550	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	3	79	theme	mass	520:523	arg1	MSI					547:549	MSI	547:549	MSI	547:549	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	0	80	theme	Late-Stage	46:55	arg1	Tissue					79:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	Early- and Late-Stage Serous Ovarian Cancer Tissue	35:84	MALDI Mass Spectrometry Imaging of Early- and Late-Stage Serous Ovarian Cancer Tissue Reveals Stage-Specific N-Glycans.					
31364262	6	81	theme	relative	1321:1328	arg1	patients					1312:1319	late-stage ovarian cancer patients	1286:1319	late-stage ovarian cancer patients relative to early-stage patients	1286:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	2	82	theme	N-glycan	400:407	arg1	alterations					409:419	tumor-specific N-glycan alterations	385:419	tumor-specific N-glycan alterations in ovarian cancer development and progression	385:465	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	8	83	theme	late-stage	1644:1653	arg1	patients					1655:1662	early- and late-stage patients	1633:1662	early- and late-stage patients	1633:1662	The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients.					
31364262	3	84	theme	late-stage	684:693	arg1	patients					695:702	early- and late-stage patients	673:702	early- and late-stage patients	673:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	6	85	theme	cancer	1305:1310	arg1	patients					1312:1319	late-stage ovarian cancer patients	1286:1319	late-stage ovarian cancer patients relative to early-stage patients	1286:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	3	86	theme	N-glycan	579:586	arg1	distribution					588:599	N-glycan distribution	579:599	N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients	579:702	matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients.					
31364262	6	87	theme	late-stage	1286:1295	arg1	patients					1312:1319	late-stage ovarian cancer patients	1286:1319	late-stage ovarian cancer patients relative to early-stage patients	1286:1352	The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients.					
31364262	2	88	theme	cancer	432:437	arg1	development					439:449	ovarian cancer development	424:449	ovarian cancer development	424:449	As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression.					
31364262	7	89	theme	diagnostic	1374:1383	arg1	markers					1385:1391	Potential N-glycan diagnostic markers	1355:1391	Potential N-glycan diagnostic markers that emerge	1355:1403	Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)6 + (Man)3 (GlcNAc)2 , and the complex neutral structure, (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2 .					
30902814	8	0	theme	N-glycan	1172:1179	arg1	subtypes					1181:1188	N-glycan subtypes	1172:1188	N-glycan subtypes	1172:1188	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	10	1	with	results	1441:1447	arg1	structure					1380:1388	the recently published cryo-EM structure	1349:1388	the recently published cryo-EM structure of the M41 IBV spike protein	1349:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	4	2	theme	strain	561:566	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	10	3	theme	protein	1411:1417	arg1	structure					1380:1388	the recently published cryo-EM structure	1349:1388	the recently published cryo-EM structure of the M41 IBV spike protein	1349:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	4	4	theme	modification	616:627	arg1	role					603:606	the role	599:606	the role of this modification in host receptor binding	599:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	11	5	theme	glycosylation-dependent	1702:1724	arg1	modality					1734:1741	a unique glycosylation-dependent binding modality	1693:1741	a unique glycosylation-dependent binding modality for the M41 spike protein	1693:1767	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	9	6	theme	most	1230:1233	arg1	RBDs					1243:1246	most variant RBDs	1230:1246	most variant RBDs	1230:1246	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	2	7	theme	spike	259:263	arg1	responsible					276:286	responsible	276:286	responsible	276:286	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	2	7	theme	spike	259:263	arg1	protein					265:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	9	8	gly	glycosylation	1204:1216	arg1	RBDs					1243:1246	most variant RBDs	1230:1246	most variant RBDs	1230:1246	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	4	9	theme	host	632:635	arg1	binding					646:652	host receptor binding	632:652	host receptor binding	632:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	9	10	theme	RBDs	1243:1246	arg1	patterns					1218:1225	the glycosylation patterns	1200:1225	the glycosylation patterns of most variant RBDs	1200:1246	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	9	10	theme	RBDs	1243:1246	arg1	similar					1260:1266	similar	1260:1266	similar	1260:1266	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	5	11	theme	Asn-to-Ala	666:675	arg1	substitutions					677:689	Ten single Asn-to-Ala substitutions	655:689	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD	655:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	10	12	gly	glycosylation	1514:1526	arg2	four					1509:1512	four	1509:1512	four	1509:1512	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	10	12	gly	glycosylation	1514:1526	arg2	sites					1528:1532	four glycosylation sites	1509:1532	four glycosylation sites that dramatically impact ligand binding	1509:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	7	13	theme	oligosaccharide	1059:1073	arg1	ligand					1075:1080	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	1	14	theme	respiratory	196:206	arg1	pathogens					208:216	important respiratory pathogens	186:216	important respiratory pathogens of poultry	186:227	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	14	theme	respiratory	196:206	arg1	coronaviruses					122:134	Avian coronaviruses	116:134	Avian coronaviruses	116:134	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	14	theme	respiratory	196:206	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	7	15	theme	sialic	1047:1052	arg1	acid					1054:1057	an ELISA-presented α2,3-linked sialic acid	1016:1057	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	7	16	theme	chicken	989:995	arg1	tissue					1005:1010	chicken trachea tissue	989:1010	chicken trachea tissue	989:1010	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	6	17	theme	M41-RBD	917:923	arg1	construct					925:933	the unmodified M41-RBD construct	902:933	the unmodified M41-RBD construct	902:933	CD analysis revealed that the secondary structure of all variants was retained compared with the unmodified M41-RBD construct.					
30902814	7	18	theme	ELISA-presented	1019:1033	arg1	acid					1054:1057	an ELISA-presented α2,3-linked sialic acid	1016:1057	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	9	19	theme	unmodified	1284:1293	arg1	construct					1303:1311	the unmodified M41-RBD construct	1280:1311	the unmodified M41-RBD construct	1280:1311	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	2	20	theme	glycosylated	242:253	arg1	responsible					276:286	responsible	276:286	responsible	276:286	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	2	20	theme	glycosylated	242:253	arg1	protein					265:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	3	21	theme	Glycosylation	317:329	arg1	sites					331:335	Glycosylation sites	317:335	Glycosylation sites in the spike protein	317:356	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	0	22	theme	coronavirus	55:65	arg1	protein					38:44	the viral attachment protein	17:44	the viral attachment protein of avian coronavirus	17:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	7	23	theme	variants	964:971	arg1	variants					964:971	the 10 glycosylation variants	943:971	the 10 glycosylation variants	943:971	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	7	23	theme	variants	964:971	arg1	Six					936:938	Six	936:938	Six	936:938	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	1	24	theme	bronchitis	158:167	arg1	IBV					176:178	IBV	176:178	IBV	176:178	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	24	theme	bronchitis	158:167	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	3	25	theme	spike	344:348	arg1	protein					350:356	the spike protein	340:356	the spike protein	340:356	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	10	26	theme	ligand	1470:1475	arg1	site					1486:1489	a potential ligand receptor site	1458:1489	a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding	1458:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	0	27	theme	host	84:87	arg1	cell					89:92	host cell	84:92	host cell	84:92	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	10	28	theme	In	1314:1315	arg1	experiments					1332:1342	In silico docking experiments	1314:1342	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein	1314:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	5	29	theme	Val-to-Ala	783:792	arg1	substitutions					794:806	two control Val-to-Ala substitutions	771:806	two control Val-to-Ala substitutions	771:806	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	3	30	from	sites	331:335	arg1	protein					350:356	the spike protein	340:356	the spike protein	340:356	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	4	31	theme	receptor-binding	524:539	arg1	domain					541:546	the receptor-binding domain	520:546	the receptor-binding domain (RBD) of IBV strain M41 spike protein	520:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	31	theme	receptor-binding	524:539	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	31	theme	receptor-binding	524:539	arg1	RBD					549:551	RBD	549:551	RBD	549:551	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	8	32	gly	glycosylation	1123:1135	arg2	sites					1137:1141	glycosylation sites	1123:1141	glycosylation sites	1123:1141	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	8	33	theme	LC/MSE	1083:1088	arg1	analysis					1100:1107	LC/MSE glycomics analysis	1083:1107	LC/MSE glycomics analysis	1083:1107	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	10	34	theme	glycosylation	1514:1526	arg1	sites					1528:1532	four glycosylation sites	1509:1532	four glycosylation sites that dramatically impact ligand binding	1509:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	10	35	theme	docking	1324:1330	arg1	experiments					1332:1342	In silico docking experiments	1314:1342	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein	1314:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	0	36	theme	attachment	27:36	arg1	protein					38:44	the viral attachment protein	17:44	the viral attachment protein of avian coronavirus	17:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	6	37	theme	secondary	839:847	arg1	structure					849:857	the secondary structure	835:857	the secondary structure of all variants	835:873	CD analysis revealed that the secondary structure of all variants was retained compared with the unmodified M41-RBD construct.					
30902814	3	38	theme	viral	386:390	arg1	genotypes					392:400	viral genotypes	386:400	viral genotypes	386:400	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	11	39	theme	M41	1751:1753	arg1	protein					1761:1767	the M41 spike protein	1747:1767	the M41 spike protein	1747:1767	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	10	40	theme	cryo-EM	1372:1378	arg1	structure					1380:1388	the recently published cryo-EM structure	1349:1388	the recently published cryo-EM structure of the M41 IBV spike protein	1349:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	11	41	theme	previous	1604:1611	arg1	studies					1619:1625	previous array studies	1604:1625	previous array studies	1604:1625	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	7	42	link	α2,3-linked	1035:1045	arg1	acid					1054:1057	an ELISA-presented α2,3-linked sialic acid	1016:1057	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	4	43	theme	M41	568:570	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	10	44	theme	M41	1397:1399	arg1	protein					1411:1417	the M41 IBV spike protein	1393:1417	the M41 IBV spike protein	1393:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	1	45	theme	poultry	221:227	arg1	pathogens					208:216	important respiratory pathogens	186:216	important respiratory pathogens of poultry	186:227	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	45	theme	poultry	221:227	arg1	coronaviruses					122:134	Avian coronaviruses	116:134	Avian coronaviruses	116:134	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	45	theme	poultry	221:227	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	8	46	theme	specific	1148:1155	arg1	proportions					1157:1167	specific proportions	1148:1167	specific proportions of N-glycan subtypes	1148:1188	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	6	47	theme	unmodified	906:915	arg1	construct					925:933	the unmodified M41-RBD construct	902:933	the unmodified M41-RBD construct	902:933	CD analysis revealed that the secondary structure of all variants was retained compared with the unmodified M41-RBD construct.					
30902814	4	48	theme	protein	578:584	arg1	domain					541:546	the receptor-binding domain	520:546	the receptor-binding domain (RBD) of IBV strain M41 spike protein	520:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	48	theme	protein	578:584	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	48	theme	protein	578:584	arg1	RBD					549:551	RBD	549:551	RBD	549:551	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	11	49	theme	unique	1695:1700	arg1	modality					1734:1741	a unique glycosylation-dependent binding modality	1693:1741	a unique glycosylation-dependent binding modality for the M41 spike protein	1693:1767	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	11	50	theme	mutational	1650:1659	arg1	analyses					1661:1668	the glycosylation and mutational analyses	1628:1668	analyses	1661:1668	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	11	51	theme	array	1613:1617	arg1	studies					1619:1625	previous array studies	1604:1625	previous array studies	1604:1625	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	9	52	theme	glycosylation	1204:1216	arg1	patterns					1218:1225	the glycosylation patterns	1200:1225	the glycosylation patterns of most variant RBDs	1200:1246	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	9	52	theme	glycosylation	1204:1216	arg1	similar					1260:1266	similar	1260:1266	similar	1260:1266	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	8	53	theme	subtypes	1181:1188	arg1	proportions					1157:1167	specific proportions	1148:1167	specific proportions of N-glycan subtypes	1148:1188	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	11	54	theme	glycosylation	1632:1644	arg1	analyses					1661:1668	the glycosylation and mutational analyses	1628:1668	analyses	1661:1668	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	4	55	theme	IBV	557:559	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	10	56	theme	spike	1405:1409	arg1	protein					1411:1417	the M41 IBV spike protein	1393:1417	the M41 IBV spike protein	1393:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	6	57	theme	variants	866:873	arg1	structure					849:857	the secondary structure	835:857	the secondary structure of all variants	835:873	CD analysis revealed that the secondary structure of all variants was retained compared with the unmodified M41-RBD construct.					
30902814	2	58	gly	glycosylated	242:253	arg1	responsible					276:286	responsible	276:286	responsible	276:286	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	2	58	gly	glycosylated	242:253	arg1	protein					265:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	11	59	theme	binding	1726:1732	arg1	modality					1734:1741	a unique glycosylation-dependent binding modality	1693:1741	a unique glycosylation-dependent binding modality for the M41 spike protein	1693:1767	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	9	60	theme	variant	1235:1241	arg1	RBDs					1243:1246	most variant RBDs	1230:1246	most variant RBDs	1230:1246	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	2	61	theme	host	303:306	arg1	tissues					308:314	host tissues	303:314	host tissues	303:314	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	5	62	theme	single	659:664	arg1	substitutions					677:689	Ten single Asn-to-Ala substitutions	655:689	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD	655:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	10	63	theme	glycosylation	1427:1439	arg1	results					1441:1447	our glycosylation results	1423:1447	our glycosylation results	1423:1447	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	4	64	from	role	603:606	arg1	binding					646:652	host receptor binding	632:652	host receptor binding	632:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	11	65	theme	studies	1619:1625	arg1	results					1593:1599	the results	1589:1599	the results of previous array studies	1589:1625	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	7	66	theme	acid	1054:1057	arg1	ligand					1075:1080	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	5	67	from	sites	724:728	arg1	substitutions					677:689	Ten single Asn-to-Ala substitutions	655:689	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD	655:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	4	68	mod	modification	616:627	arg1	receptor					637:644	host receptor binding	632:652	host receptor binding	632:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	68	mod	modification	616:627	arg1	host					632:635	host receptor binding	632:652	host receptor binding	632:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	1	69	theme	important	186:194	arg1	pathogens					208:216	important respiratory pathogens	186:216	important respiratory pathogens of poultry	186:227	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	69	theme	important	186:194	arg1	coronaviruses					122:134	Avian coronaviruses	116:134	Avian coronaviruses	116:134	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	69	theme	important	186:194	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	7	70	theme	α2,3-linked	1035:1045	arg1	acid					1054:1057	an ELISA-presented α2,3-linked sialic acid	1016:1057	an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand	1016:1080	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	2	71	theme	IBV	255:257	arg1	responsible					276:286	responsible	276:286	responsible	276:286	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	2	71	theme	IBV	255:257	arg1	protein					265:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein	230:271	The heavily glycosylated IBV spike protein is responsible for binding to host tissues.					
30902814	7	72	theme	trachea	997:1003	arg1	tissue					1005:1010	chicken trachea tissue	989:1010	chicken trachea tissue	989:1010	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	1	73	theme	Avian	116:120	arg1	pathogens					208:216	important respiratory pathogens	186:216	important respiratory pathogens of poultry	186:227	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	73	theme	Avian	116:120	arg1	coronaviruses					122:134	Avian coronaviruses	116:134	Avian coronaviruses	116:134	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	73	theme	Avian	116:120	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	3	74	theme	virus	461:465	arg1	cycle					472:476	the virus life cycle	457:476	the virus life cycle	457:476	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	9	75	theme	M41-RBD	1295:1301	arg1	construct					1303:1311	the unmodified M41-RBD construct	1280:1311	the unmodified M41-RBD construct	1280:1311	Overall, the glycosylation patterns of most variant RBDs were highly similar to those of the unmodified M41-RBD construct.					
30902814	4	76	gly	N-glycosylation	501:515	arg1	domain					541:546	the receptor-binding domain	520:546	the receptor-binding domain (RBD) of IBV strain M41 spike protein	520:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	76	gly	N-glycosylation	501:515	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	76	gly	N-glycosylation	501:515	arg1	RBD					549:551	RBD	549:551	RBD	549:551	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	4	77	theme	receptor	637:644	arg1	binding					646:652	host receptor binding	632:652	host receptor binding	632:652	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	5	78	theme	predicted	698:706	arg1	sites					724:728	the predicted N-glycosylation sites	694:728	the predicted N-glycosylation sites of the M41-RBD	694:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	78	theme	predicted	698:706	arg1	M41-RBD					737:743	the M41-RBD	733:743	the M41-RBD	733:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	79	theme	M41-RBD	737:743	arg1	sites					724:728	the predicted N-glycosylation sites	694:728	the predicted N-glycosylation sites of the M41-RBD	694:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	79	theme	M41-RBD	737:743	arg1	M41-RBD					737:743	the M41-RBD	733:743	the M41-RBD	733:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	10	80	dep	In	1314:1315	arg1	silico					1317:1322	silico	1317:1322	silico	1317:1322	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	1	81	theme	infectious	147:156	arg1	IBV					176:178	IBV	176:178	IBV	176:178	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	1	81	theme	infectious	147:156	arg1	virus					169:173	infectious bronchitis virus	147:173	infectious bronchitis virus (IBV)	147:179	Avian coronaviruses, including infectious bronchitis virus (IBV), are important respiratory pathogens of poultry.					
30902814	10	82	theme	receptor	1477:1484	arg1	site					1486:1489	a potential ligand receptor site	1458:1489	a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding	1458:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	7	83	theme	glycosylation	950:962	arg1	variants					964:971	the 10 glycosylation variants	943:971	the 10 glycosylation variants	943:971	Six of the 10 glycosylation variants lost binding to chicken trachea tissue and an ELISA-presented α2,3-linked sialic acid oligosaccharide ligand.					
30902814	10	84	theme	potential	1460:1468	arg1	site					1486:1489	a potential ligand receptor site	1458:1489	a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding	1458:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	0	85	theme	receptor	98:105	arg1	binding					107:113	receptor binding	98:113	receptor binding	98:113	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	10	86	with	experiments	1332:1342	arg1	structure					1380:1388	the recently published cryo-EM structure	1349:1388	the recently published cryo-EM structure of the M41 IBV spike protein	1349:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	5	87	theme	N-glycosylation	708:722	arg1	sites					724:728	the predicted N-glycosylation sites	694:728	the predicted N-glycosylation sites of the M41-RBD	694:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	87	theme	N-glycosylation	708:722	arg1	M41-RBD					737:743	the M41-RBD	733:743	the M41-RBD	733:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	88	theme	control	775:781	arg1	substitutions					794:806	two control Val-to-Ala substitutions	771:806	two control Val-to-Ala substitutions	771:806	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	4	89	theme	domain	541:546	arg1	N-glycosylation					501:515	the N-glycosylation	497:515	the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein	497:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	3	90	theme	important	417:425	arg1	role					427:430	an important role	414:430	an important role for this modification in the virus life cycle	414:476	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	0	91	theme	viral	21:25	arg1	protein					38:44	the viral attachment protein	17:44	the viral attachment protein of avian coronavirus	17:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	8	92	theme	glycomics	1090:1098	arg1	analysis					1100:1107	LC/MSE glycomics analysis	1083:1107	LC/MSE glycomics analysis	1083:1107	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	11	93	theme	spike	1755:1759	arg1	protein					1761:1767	the M41 spike protein	1747:1767	the M41 spike protein	1747:1767	Combined with the results of previous array studies, the glycosylation and mutational analyses presented here suggest a unique glycosylation-dependent binding modality for the M41 spike protein.					
30902814	5	94	gly	N-glycosylation	708:722	arg1	M41-RBD					737:743	the M41-RBD	733:743	the M41-RBD	733:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	94	gly	N-glycosylation	708:722	arg2	M41-RBD					737:743	the M41-RBD	733:743	the M41-RBD	733:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	5	94	gly	N-glycosylation	708:722	arg2	sites					724:728	the predicted N-glycosylation sites	694:728	the predicted N-glycosylation sites of the M41-RBD	694:743	Ten single Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along with two control Val-to-Ala substitutions.					
30902814	0	95	theme	protein	38:44	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of the viral attachment protein of avian coronavirus	0:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	0	96	gly	Glycosylation	0:12	arg1	protein					38:44	the viral attachment protein	17:44	the viral attachment protein of avian coronavirus	17:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	0	97	theme	avian	49:53	arg1	coronavirus					55:65	avian coronavirus	49:65	avian coronavirus	49:65	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding.					
30902814	8	98	theme	glycosylation	1123:1135	arg1	sites					1137:1141	glycosylation sites	1123:1141	glycosylation sites	1123:1141	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	10	99	theme	ligand	1559:1564	arg1	binding					1566:1572	ligand binding	1559:1572	ligand binding	1559:1572	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	10	100	theme	published	1362:1370	arg1	structure					1380:1388	the recently published cryo-EM structure	1349:1388	the recently published cryo-EM structure of the M41 IBV spike protein	1349:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	8	101	contain	have	1143:1146	arg1	sites					1137:1141	glycosylation sites	1123:1141	glycosylation sites	1123:1141	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	8	101	contain	have	1143:1146	arg2	proportions					1157:1167	specific proportions	1148:1167	specific proportions of N-glycan subtypes	1148:1188	LC/MSE glycomics analysis revealed that glycosylation sites have specific proportions of N-glycan subtypes.					
30902814	4	102	theme	spike	572:576	arg1	protein					578:584	IBV strain M41 spike protein	557:584	IBV strain M41 spike protein	557:584	Here, we analyzed the N-glycosylation of the receptor-binding domain (RBD) of IBV strain M41 spike protein and assessed the role of this modification in host receptor binding.					
30902814	10	103	theme	IBV	1401:1403	arg1	protein					1411:1417	the M41 IBV spike protein	1393:1417	the M41 IBV spike protein	1393:1417	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding.					
30902814	3	104	theme	life	467:470	arg1	cycle					472:476	the virus life cycle	457:476	the virus life cycle	457:476	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
30902814	6	105	theme	CD	809:810	arg1	analysis					812:819	CD analysis	809:819	CD analysis	809:819	CD analysis revealed that the secondary structure of all variants was retained compared with the unmodified M41-RBD construct.					
30902814	3	106	from	role	427:430	arg1	cycle					472:476	the virus life cycle	457:476	the virus life cycle	457:476	Glycosylation sites in the spike protein are highly conserved across viral genotypes, suggesting an important role for this modification in the virus life cycle.					
31548313	11	0	theme	similar	1740:1746	arg1	pocket					1763:1768	a similar fucose-binding pocket	1738:1768	a similar fucose-binding pocket	1738:1768	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	12	1	theme	protein	1864:1870	arg1	engineering					1872:1882	protein engineering	1864:1882	protein engineering	1864:1882	Our study provides a foothold for protein engineering to develop enzymatic tools for the preparation of more effective therapeutic antibodies.					
31548313	11	2	theme	surrounding	1779:1789	arg1	residues					1791:1798	the surrounding residues	1775:1798	the surrounding residues	1775:1798	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	8	3	theme	arginine	1335:1342	arg1	residues					1344:1351	several lysine and arginine residues	1316:1351	several lysine and arginine residues	1316:1351	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	9	4	theme	sialobiantennary	1535:1550	arg1	substrate					1552:1560	a sialobiantennary substrate	1533:1560	a sialobiantennary substrate	1533:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	8	5	theme	Endo-CoM	1290:1297	arg1	Y-shaped					1302:1309	Y-shaped	1302:1309	Y-shaped	1302:1309	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	8	5	theme	Endo-CoM	1290:1297	arg1	cleft					1281:1285	The N-glycan-binding cleft	1260:1285	The N-glycan-binding cleft of Endo-CoM	1260:1297	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	4	6	theme	asparagine-linked	756:772	arg1	GlcNAc					774:779	the asparagine-linked GlcNAc	752:779	the asparagine-linked GlcNAc	752:779	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	7	7	theme	asparagine	1194:1203	arg1	moiety					1205:1210	the asparagine moiety	1190:1210	the asparagine moiety linked to the GlcNAc	1190:1231	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	11	8	contain	has	1734:1736	arg1	Endo-F3					1688:1694	Endo-F3	1688:1694	Endo-F3	1688:1694	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	11	8	contain	has	1734:1736	arg1	ENGase					1726:1731	a known core fucose-specific ENGase	1697:1731	a known core fucose-specific ENGase	1697:1731	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	11	8	contain	has	1734:1736	arg2	pocket					1763:1768	a similar fucose-binding pocket	1738:1768	a similar fucose-binding pocket	1738:1768	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	4	9	link	asparagine-linked	756:772	arg1	GlcNAc					774:779	the asparagine-linked GlcNAc	752:779	the asparagine-linked GlcNAc	752:779	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	4	10	theme	core	736:739	arg1	fucose					741:746	the core fucose	732:746	the core fucose	732:746	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	8	11	theme	lysine	1324:1329	arg1	residues					1344:1351	several lysine and arginine residues	1316:1351	several lysine and arginine residues	1316:1351	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	1	12	theme	N-Linked	144:151	arg1	glycans					153:159	N-Linked glycans	144:159	N-Linked glycans	144:159	N-Linked glycans play important roles in various cellular and immunological events.					
31548313	5	13	theme	core	843:846	arg1	ENGase					864:869	a core fucose-specific ENGase	841:869	a core fucose-specific ENGase	841:869	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	7	14	attach	linked	1093:1098	arg2	moiety					1086:1091	The fucose moiety	1075:1091	The fucose moiety linked to GlcNAc	1075:1108	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	7	14	attach	linked	1093:1098	arg1	GlcNAc					1103:1108	GlcNAc	1103:1108	GlcNAc	1103:1108	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	5	15	theme	ENGase	864:869	arg1	structures					827:836	the crystal structures	815:836	the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18	815:976	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	1	16	theme	various	185:191	arg1	events					220:225	various cellular and immunological events	185:225	various cellular and immunological events	185:225	N-Linked glycans play important roles in various cellular and immunological events.					
31548313	5	17	theme	crystal	819:825	arg1	structures					827:836	the crystal structures	815:836	the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18	815:976	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	0	18	theme	Cordyceps	123:131	arg1	militaris					133:141	the fungus Cordyceps militaris	112:141	the fungus Cordyceps militaris	112:141	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	0	19	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.	0:142	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	9	20	from	activity	1441:1448	arg1	glycans					1483:1489	fucose-containing glycans	1465:1489	fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate	1465:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	4	21	theme	various	641:647	arg1	ENGases					649:655	various ENGases	641:655	various ENGases	641:655	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	10	22	theme	structural	1603:1612	arg1	insights					1614:1621	structural insights	1603:1621	structural insights into their core fucose tolerance and specificity	1603:1670	Comparisons with other ENGases provided structural insights into their core fucose tolerance and specificity.					
31548313	0	23	theme	specific	25:32	arg1	cleavage					34:41	the specific cleavage	21:41	the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris	21:141	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	0	24	theme	N-glycans	63:71	arg1	cleavage					34:41	the specific cleavage	21:41	the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris	21:141	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	11	25	theme	fucose-specific	1710:1724	arg1	Endo-F3					1688:1694	Endo-F3	1688:1694	Endo-F3	1688:1694	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	11	25	theme	fucose-specific	1710:1724	arg1	ENGase					1726:1731	a known core fucose-specific ENGase	1697:1731	a known core fucose-specific ENGase	1697:1731	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	2	26	theme	modified	403:410	arg1	glycans					412:418	homogeneously modified glycans	389:418	homogeneously modified glycans	389:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	5	27	theme	fungus	892:897	arg1	militaris					909:917	the caterpillar fungus Cordyceps militaris	876:917	the caterpillar fungus Cordyceps militaris (Endo-CoM)	876:928	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	5	27	theme	fungus	892:897	arg1	Endo-CoM					920:927	Endo-CoM	920:927	Endo-CoM	920:927	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	12	28	theme	effective	1939:1947	arg1	antibodies					1961:1970	more effective therapeutic antibodies	1934:1970	more effective therapeutic antibodies	1934:1970	Our study provides a foothold for protein engineering to develop enzymatic tools for the preparation of more effective therapeutic antibodies.					
31548313	7	29	attach	linked	1212:1217	arg1	GlcNAc					1226:1231	the GlcNAc	1222:1231	the GlcNAc	1222:1231	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	7	29	attach	linked	1212:1217	arg2	moiety					1205:1210	the asparagine moiety	1190:1210	the asparagine moiety linked to the GlcNAc	1190:1231	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	10	30	theme	core	1634:1637	arg1	fucose					1639:1644	their core fucose tolerance and specificity	1628:1670	fucose	1639:1644	Comparisons with other ENGases provided structural insights into their core fucose tolerance and specificity.					
31548313	11	31	theme	known	1699:1703	arg1	Endo-F3					1688:1694	Endo-F3	1688:1694	Endo-F3	1688:1694	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	11	31	theme	known	1699:1703	arg1	ENGase					1726:1731	a known core fucose-specific ENGase	1697:1731	a known core fucose-specific ENGase	1697:1731	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	3	32	theme	mammalian	541:549	arg1	N-glycans					551:559	mammalian N-glycans	541:559	mammalian N-glycans	541:559	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	4	33	theme	structural	692:701	arg1	unclear					784:790	unclear	784:790	unclear	784:790	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	4	33	theme	structural	692:701	arg1	basis					703:707	the structural basis	688:707	the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc	688:779	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	5	34	theme	glycoside	948:956	arg1	family					968:973	glycoside hydrolase family 18	948:976	glycoside hydrolase family 18	948:976	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	0	35	theme	core-fucosylated	46:61	arg1	N-glycans					63:71	core-fucosylated N-glycans	46:71	core-fucosylated N-glycans	46:71	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	0	36	gly	core-fucosylated	46:61	arg1	N-glycans					63:71	core-fucosylated N-glycans	46:71	core-fucosylated N-glycans	46:71	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	6	37	theme	fucose-containing	1005:1021	arg1	ligands					1023:1029	fucose-containing ligands	1005:1029	fucose-containing ligands	1005:1029	Structures complexed with fucose-containing ligands were determined at 1.75-2.35 Å resolutions.					
31548313	11	38	theme	fucose-binding	1748:1761	arg1	pocket					1763:1768	a similar fucose-binding pocket	1738:1768	a similar fucose-binding pocket	1738:1768	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	8	39	attach	present	1357:1363	arg1	regions					1381:1387	its terminal regions	1368:1387	its terminal regions	1368:1387	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	8	39	attach	present	1357:1363	arg2	residues					1344:1351	several lysine and arginine residues	1316:1351	several lysine and arginine residues	1316:1351	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	2	40	theme	promising	319:327	arg1	tool					329:332	a promising tool	317:332	a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans	317:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	5	41	theme	hydrolase	958:966	arg1	family					968:973	glycoside hydrolase family 18	948:976	glycoside hydrolase family 18	948:976	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	1	42	theme	cellular	193:200	arg1	events					220:225	various cellular and immunological events	185:225	various cellular and immunological events	185:225	N-Linked glycans play important roles in various cellular and immunological events.					
31548313	8	43	theme	N-glycan-binding	1264:1279	arg1	Y-shaped					1302:1309	Y-shaped	1302:1309	Y-shaped	1302:1309	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	8	43	theme	N-glycan-binding	1264:1279	arg1	cleft					1281:1285	The N-glycan-binding cleft	1260:1285	The N-glycan-binding cleft of Endo-CoM	1260:1297	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	4	44	theme	fucose	741:746	arg1	recognition					717:727	the recognition	713:727	the recognition of the core fucose	713:746	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	4	44	theme	fucose	741:746	arg1	GlcNAc					774:779	the asparagine-linked GlcNAc	752:779	the asparagine-linked GlcNAc	752:779	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	10	45	dep	fucose	1639:1644	arg1	tolerance					1646:1654	tolerance	1646:1654	tolerance	1646:1654	Comparisons with other ENGases provided structural insights into their core fucose tolerance and specificity.					
31548313	1	46	theme	immunological	206:218	arg1	events					220:225	various cellular and immunological events	185:225	various cellular and immunological events	185:225	N-Linked glycans play important roles in various cellular and immunological events.					
31548313	2	47	gly	transglycosylate	283:298	arg1	N-glycans					300:308	N-glycans	300:308	N-glycans	300:308	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	2	48	theme	chemoenzymatic	342:355	arg1	synthesis					357:365	the chemoenzymatic synthesis	338:365	the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans	338:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	7	49	theme	round-shaped	1161:1172	arg1	pocket					1174:1179	a round-shaped pocket	1159:1179	a round-shaped pocket	1159:1179	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	6	50	theme	Å	1060:1060	arg1	resolutions					1062:1072	1.75-2.35 Å resolutions	1050:1072	1.75-2.35 Å resolutions	1050:1072	Structures complexed with fucose-containing ligands were determined at 1.75-2.35 Å resolutions.					
31548313	8	51	theme	terminal	1372:1379	arg1	regions					1381:1387	its terminal regions	1368:1387	its terminal regions	1368:1387	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	2	52	gly	glycoproteins	370:382	arg1	glycoproteins					370:382	glycoproteins	370:382	glycoproteins with homogeneously modified glycans	370:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	8	53	theme	several	1316:1322	arg1	residues					1344:1351	several lysine and arginine residues	1316:1351	several lysine and arginine residues	1316:1351	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	0	54	from	militaris	133:141	arg1	endo-β-N-acetylglucosaminidase					76:105	endo-β-N-acetylglucosaminidase	76:105	endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris	76:141	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	9	55	with	consistent	1421:1430	arg1	activity					1441:1448	the activity	1437:1448	the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate	1437:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	7	56	theme	protein	1139:1145	arg1	residues					1147:1154	protein residues	1139:1154	protein residues in a round-shaped pocket	1139:1179	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	1	57	theme	important	166:174	arg1	roles					176:180	important roles	166:180	important roles	166:180	N-Linked glycans play important roles in various cellular and immunological events.					
31548313	7	58	from	residues	1147:1154	arg1	pocket					1174:1179	a round-shaped pocket	1159:1179	a round-shaped pocket	1159:1179	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	5	59	theme	fucose-specific	848:862	arg1	ENGase					864:869	a core fucose-specific ENGase	841:869	a core fucose-specific ENGase	841:869	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	9	60	from	glycans	1483:1489	arg1	IgG					1505:1507	IgG	1505:1507	IgG	1505:1507	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	9	60	from	glycans	1483:1489	arg1	rituximab					1494:1502	rituximab	1494:1502	rituximab (IgG)	1494:1508	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	9	60	from	glycans	1483:1489	arg1	preference					1518:1527	its preference	1514:1527	its preference for a sialobiantennary substrate	1514:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	0	61	theme	fungus	116:121	arg1	militaris					133:141	the fungus Cordyceps militaris	112:141	the fungus Cordyceps militaris	112:141	Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.					
31548313	4	62	dep	biochemistries	608:621	arg1	the					604:606	the	604:606	the	604:606	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	3	63	gly	core-fucosylated	454:469	arg1	glycans					471:477	core-fucosylated glycans	454:477	core-fucosylated glycans	454:477	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	7	64	theme	fucose	1079:1084	arg1	moiety					1086:1091	The fucose moiety	1075:1091	The fucose moiety linked to GlcNAc	1075:1108	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	9	65	theme	structural	1396:1405	arg1	features					1407:1414	These structural features	1390:1414	These structural features	1390:1414	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	8	66	located	present	1357:1363	arg1	regions					1381:1387	its terminal regions	1368:1387	its terminal regions	1368:1387	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	8	66	located	present	1357:1363	arg2	residues					1344:1351	several lysine and arginine residues	1316:1351	several lysine and arginine residues	1316:1351	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	8	67	from	regions	1381:1387	arg1	present					1357:1363	present	1357:1363	present	1357:1363	The N-glycan-binding cleft of Endo-CoM is Y-shaped, and several lysine and arginine residues are present at its terminal regions.					
31548313	10	68	theme	other	1580:1584	arg1	ENGases					1586:1592	other ENGases	1580:1592	other ENGases	1580:1592	Comparisons with other ENGases provided structural insights into their core fucose tolerance and specificity.					
31548313	3	69	gly	α-1,6-fucosylated	576:592	arg1	N-glycans					551:559	mammalian N-glycans	541:559	mammalian N-glycans	541:559	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	7	70	dep	recognized	1125:1134	arg1	whereas					1182:1188	whereas	1182:1188	whereas	1182:1188	The fucose moiety linked to GlcNAc is extensively recognized by protein residues in a round-shaped pocket, whereas the asparagine moiety linked to the GlcNAc is exposed to the solvent.					
31548313	5	71	theme	Cordyceps	899:907	arg1	militaris					909:917	the caterpillar fungus Cordyceps militaris	876:917	the caterpillar fungus Cordyceps militaris (Endo-CoM)	876:928	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	5	71	theme	Cordyceps	899:907	arg1	Endo-CoM					920:927	Endo-CoM	920:927	Endo-CoM	920:927	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	5	72	from	militaris	909:917	arg1	structures					827:836	the crystal structures	815:836	the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18	815:976	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	4	73	theme	ENGases	649:655	arg1	structures					627:636	structures	627:636	structures	627:636	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	4	73	theme	ENGases	649:655	arg1	biochemistries					608:621	biochemistries	608:621	biochemistries	608:621	Although the biochemistries and structures of various ENGases have been studied extensively, the structural basis for the recognition of the core fucose and the asparagine-linked GlcNAc is unclear.					
31548313	12	74	dep	effective	1939:1947	arg1	therapeutic					1949:1959	therapeutic	1949:1959	therapeutic	1949:1959	Our study provides a foothold for protein engineering to develop enzymatic tools for the preparation of more effective therapeutic antibodies.					
31548313	3	75	theme	key	484:486	arg1	factor					488:493	a key factor	482:493	a key factor determining their therapeutic utility	482:531	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	3	75	theme	key	484:486	arg1	ability					425:431	The ability	421:431	The ability of ENGases to act on core-fucosylated glycans	421:477	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	10	76	with	Comparisons	1563:1573	arg1	ENGases					1586:1592	other ENGases	1580:1592	other ENGases	1580:1592	Comparisons with other ENGases provided structural insights into their core fucose tolerance and specificity.					
31548313	3	77	theme	ENGases	436:442	arg1	factor					488:493	a key factor	482:493	a key factor determining their therapeutic utility	482:531	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	3	77	theme	ENGases	436:442	arg1	ability					425:431	The ability	421:431	The ability of ENGases to act on core-fucosylated glycans	421:477	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	9	78	theme	Endo-CoM	1453:1460	arg1	activity					1441:1448	the activity	1437:1448	the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate	1437:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	2	79	with	glycoproteins	370:382	arg1	glycans					412:418	homogeneously modified glycans	389:418	homogeneously modified glycans	389:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	11	80	theme	core	1705:1708	arg1	Endo-F3					1688:1694	Endo-F3	1688:1694	Endo-F3	1688:1694	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	11	80	theme	core	1705:1708	arg1	ENGase					1726:1731	a known core fucose-specific ENGase	1697:1731	a known core fucose-specific ENGase	1697:1731	In particular, Endo-F3, a known core fucose-specific ENGase, has a similar fucose-binding pocket, but the surrounding residues are not shared with Endo-CoM.					
31548313	3	81	theme	therapeutic	513:523	arg1	utility					525:531	their therapeutic utility	507:531	their therapeutic utility	507:531	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	5	82	theme	caterpillar	880:890	arg1	militaris					909:917	the caterpillar fungus Cordyceps militaris	876:917	the caterpillar fungus Cordyceps militaris (Endo-CoM)	876:928	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	5	82	theme	caterpillar	880:890	arg1	Endo-CoM					920:927	Endo-CoM	920:927	Endo-CoM	920:927	Herein, we determined the crystal structures of a core fucose-specific ENGase from the caterpillar fungus Cordyceps militaris (Endo-CoM), which belongs to glycoside hydrolase family 18.					
31548313	9	83	theme	fucose-containing	1465:1481	arg1	glycans					1483:1489	fucose-containing glycans	1465:1489	fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate	1465:1560	These structural features were consistent with the activity of Endo-CoM on fucose-containing glycans on rituximab (IgG) and its preference for a sialobiantennary substrate.					
31548313	12	84	theme	antibodies	1961:1970	arg1	preparation					1919:1929	the preparation	1915:1929	the preparation of more effective therapeutic antibodies	1915:1970	Our study provides a foothold for protein engineering to develop enzymatic tools for the preparation of more effective therapeutic antibodies.					
31548313	3	85	theme	core-fucosylated	454:469	arg1	glycans					471:477	core-fucosylated glycans	454:477	core-fucosylated glycans	454:477	The ability of ENGases to act on core-fucosylated glycans is a key factor determining their therapeutic utility because mammalian N-glycans are frequently α-1,6-fucosylated.					
31548313	2	86	theme	glycoproteins	370:382	arg1	synthesis					357:365	the chemoenzymatic synthesis	338:365	the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans	338:418	Endo-β-N-acetylglucosaminidase (ENGase) can release or transglycosylate N-glycans and is a promising tool for the chemoenzymatic synthesis of glycoproteins with homogeneously modified glycans.					
31548313	12	87	theme	enzymatic	1895:1903	arg1	tools					1905:1909	enzymatic tools	1895:1909	enzymatic tools for the preparation of more effective therapeutic antibodies	1895:1970	Our study provides a foothold for protein engineering to develop enzymatic tools for the preparation of more effective therapeutic antibodies.					
31507162	9	0	theme	potential	1652:1660	arg1	association					1662:1672	the potential association	1648:1672	the potential association of this type of N-glycans with tumor progression	1648:1721	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	6	1	theme	imaging	1238:1244	arg1	analysis					1246:1253	N-glycan imaging analysis	1229:1253	N-glycan imaging analysis	1229:1253	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	5	2	dep	in	1094:1095	arg1	situ					1097:1100	situ	1097:1100	situ	1097:1100	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	3	from	sections	1182:1189	arg1	imaging					1102:1108	in situ imaging	1094:1108	in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1094:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	3	from	sections	1182:1189	arg1	glycans					1122:1128	N-linked glycans	1113:1128	N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1113:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	4	with	source	1017:1022	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	6	5	theme	new	1212:1214	arg1	platform					1216:1223	this new platform	1207:1223	this new platform for N-glycan imaging analysis	1207:1253	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	9	6	with	association	1662:1672	arg1	progression					1711:1721	tumor progression	1705:1721	tumor progression	1705:1721	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	9	7	theme	elevated	1589:1596	arg1	levels					1609:1614	elevated expression levels	1589:1614	elevated expression levels in the tumor region	1589:1634	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	8	8	theme	FFPE	1514:1517	arg1	section					1547:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	6	9	theme	sections	1302:1309	arg1	variety					1279:1285	a variety	1277:1285	a variety of FFPE tissue sections	1277:1309	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	6	9	theme	sections	1302:1309	arg1	sections					1302:1309	FFPE tissue sections	1290:1309	FFPE tissue sections	1290:1309	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	9	10	theme	High-mannose	1556:1567	arg1	N-glycans					1569:1577	High-mannose N-glycans	1556:1577	High-mannose N-glycans	1556:1577	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	8	11	theme	ovarian	1525:1531	arg1	section					1547:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	6	12	theme	FFPE	1290:1293	arg1	sections					1302:1309	FFPE tissue sections	1290:1309	FFPE tissue sections	1290:1309	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	3	13	theme	various	647:653	arg1	diseases					655:662	various diseases	647:662	various diseases	647:662	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	5	14	theme	subatmospheric	979:992	arg1	source					1017:1022	a novel subatmospheric pressure (SubAP)/MALDI source	971:1022	a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	971:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	15	link	N-linked	1113:1120	arg1	glycans					1122:1128	N-linked glycans	1113:1128	N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1113:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	8	16	theme	tissue	1540:1545	arg1	section					1547:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	4	17	theme	biomolecules	725:736	arg1	mapping					714:720	in situ mapping	706:720	in situ mapping of biomolecules and their region-specific localizations	706:776	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	4	18	theme	laser	795:799	arg1	spectrometry					828:839	matrix-assisted laser desorption/ionization mass spectrometry	779:839	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	779:859	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	5	19	theme	N-linked	1113:1120	arg1	glycans					1122:1128	N-linked glycans	1113:1128	N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1113:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	9	20	theme	expression	1598:1607	arg1	levels					1609:1614	elevated expression levels	1589:1614	elevated expression levels in the tumor region	1589:1634	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	1	21	theme	N-linked	150:157	arg1	glycosylation					159:171	N-linked glycosylation	150:171	N-linked glycosylation	150:171	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	0	22	theme	Tissue	77:82	arg1	Sections					84:91	Formalin-Fixed Paraffin-Embedded Tissue Sections	44:91	Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source	44:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	4	23	theme	region-specific	748:762	arg1	localizations					764:776	their region-specific localizations	742:776	their region-specific localizations	742:776	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	9	24	theme	tumor	1623:1627	arg1	region					1629:1634	the tumor region	1619:1634	the tumor region	1619:1634	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	8	25	with	N-glycans	1432:1440	arg1	patterns					1478:1485	different spatial distribution patterns	1447:1485	different spatial distribution patterns	1447:1485	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	0	26	theme	Novel	101:105	arg1	Source					142:147	a Novel Subatmospheric Pressure Ionization Source	99:147	a Novel Subatmospheric Pressure Ionization Source	99:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	0	27	theme	Pressure	122:129	arg1	Source					142:147	a Novel Subatmospheric Pressure Ionization Source	99:147	a Novel Subatmospheric Pressure Ionization Source	99:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	5	28	theme	tissue	1175:1180	arg1	sections					1182:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	29	theme	Exactive	1033:1040	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	7	30	theme	brain	1401:1405	arg1	section					1407:1413	a FFPE mouse brain section	1388:1413	a FFPE mouse brain section	1388:1413	A total of 55 N-glycans were successfully characterized and visualized from a FFPE mouse brain section.					
31507162	4	31	theme	mass	823:826	arg1	spectrometry					828:839	matrix-assisted laser desorption/ionization mass spectrometry	779:839	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	779:859	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	0	32	theme	Mass	0:3	arg1	Spectrometry					5:16	Mass Spectrometry	0:16	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.	0:148	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	1	33	theme	protein	302:308	arg1	structures					310:319	protein structures	302:319	protein structures	302:319	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	7	34	theme	FFPE	1390:1393	arg1	section					1407:1413	a FFPE mouse brain section	1388:1413	a FFPE mouse brain section	1388:1413	A total of 55 N-glycans were successfully characterized and visualized from a FFPE mouse brain section.					
31507162	8	35	theme	different	1447:1455	arg1	patterns					1478:1485	different spatial distribution patterns	1447:1485	different spatial distribution patterns	1447:1485	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	3	36	theme	glycosylation	513:525	arg1	glycoforms					543:552	the glycoforms	539:552	especially the glycoforms	528:552	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	3	36	theme	glycosylation	513:525	arg1	alteration					499:508	the disease-related alteration	479:508	the disease-related alteration of glycosylation	479:525	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	8	37	theme	distribution	1465:1476	arg1	patterns					1478:1485	different spatial distribution patterns	1447:1485	different spatial distribution patterns	1447:1485	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	0	38	theme	N-Glycans	29:37	arg1	Imaging					18:24	Mass Spectrometry Imaging	0:24	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.	0:148	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	1	39	link	N-linked	150:157	arg1	glycosylation					159:171	N-linked glycosylation	150:171	N-linked glycosylation	150:171	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	4	40	theme	potential	905:913	arg1	biomarkers					915:924	potential biomarkers	905:924	potential biomarkers for many diseases	905:942	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	2	41	theme	abnormal	373:380	arg1	changes					382:388	abnormal changes	373:388	abnormal changes of protein N-glycosylation patterns	373:424	It has been revealed that abnormal changes of protein N-glycosylation patterns are associated with many diseases.					
31507162	0	42	theme	Formalin-Fixed	44:57	arg1	Sections					84:91	Formalin-Fixed Paraffin-Embedded Tissue Sections	44:91	Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source	44:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	1	43	theme	complex	234:240	arg1	PTMs					284:287	PTMs	284:287	PTMs	284:287	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	43	theme	complex	234:240	arg1	modifications					269:281	the most common and complex protein post-translational modifications	214:281	the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions	214:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	5	44	theme	hybrid	1045:1050	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	7	45	theme	N-glycans	1326:1334	arg1	total					1314:1318	A total	1312:1318	A total of 55 N-glycans	1312:1334	A total of 55 N-glycans were successfully characterized and visualized from a FFPE mouse brain section.					
31507162	2	46	theme	N-glycosylation	401:415	arg1	patterns					417:424	protein N-glycosylation patterns	393:424	protein N-glycosylation patterns	393:424	It has been revealed that abnormal changes of protein N-glycosylation patterns are associated with many diseases.					
31507162	1	47	theme	post-translational	250:267	arg1	PTMs					284:287	PTMs	284:287	PTMs	284:287	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	47	theme	post-translational	250:267	arg1	modifications					269:281	the most common and complex protein post-translational modifications	214:281	the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions	214:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	5	48	theme	mass	1072:1075	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	49	dep	formalin-fixed	1135:1148	arg1	paraffin-embedded					1150:1166	paraffin-embedded	1150:1166	paraffin-embedded	1150:1166	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	9	50	theme	N-glycans	1690:1698	arg1	type					1682:1685	this type	1677:1685	this type of N-glycans	1677:1698	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	9	51	from	levels	1609:1614	arg1	region					1629:1634	the tumor region	1619:1634	the tumor region	1619:1634	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	4	52	theme	biomarkers	915:924	arg1	discovery					892:900	the discovery	888:900	the discovery of potential biomarkers for many diseases	888:942	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	6	53	theme	N-glycan	1229:1236	arg1	analysis					1246:1253	N-glycan imaging analysis	1229:1253	N-glycan imaging analysis	1229:1253	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	4	54	theme	in	706:707	arg1	mapping					714:720	in situ mapping	706:720	in situ mapping of biomolecules and their region-specific localizations	706:776	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	3	55	theme	pathogenic	622:631	arg1	mechanisms					633:642	the pathogenic mechanisms	618:642	the pathogenic mechanisms of various diseases	618:662	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	4	56	theme	many	930:933	arg1	diseases					935:942	many diseases	930:942	many diseases	930:942	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	6	57	theme	platform	1216:1223	arg1	utility					1196:1202	The utility	1192:1202	The utility of this new platform for N-glycan imaging analysis	1192:1253	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	4	58	theme	mapping	714:720	arg1	capability					692:701	the capability	688:701	the capability of in situ mapping of biomolecules and their region-specific localizations	688:776	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	8	59	theme	mouse	1519:1523	arg1	section					1547:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	5	60	theme	formalin-fixed	1135:1148	arg1	sections					1182:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	3	61	theme	diseases	655:662	arg1	mechanisms					633:642	the pathogenic mechanisms	618:642	the pathogenic mechanisms of various diseases	618:662	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	1	62	theme	common	223:228	arg1	PTMs					284:287	PTMs	284:287	PTMs	284:287	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	62	theme	common	223:228	arg1	modifications					269:281	the most common and complex protein post-translational modifications	214:281	the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions	214:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	0	63	from	Sections	84:91	arg1	Imaging					18:24	Mass Spectrometry Imaging	0:24	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.	0:148	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	6	64	theme	tissue	1295:1300	arg1	sections					1302:1309	FFPE tissue sections	1290:1309	FFPE tissue sections	1290:1309	The utility of this new platform for N-glycan imaging analysis was demonstrated with a variety of FFPE tissue sections.					
31507162	5	65	theme	novel	973:977	arg1	source					1017:1022	a novel subatmospheric pressure (SubAP)/MALDI source	971:1022	a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	971:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	4	66	theme	localizations	764:776	arg1	mapping					714:720	in situ mapping	706:720	in situ mapping of biomolecules and their region-specific localizations	706:776	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	9	67	theme	tumor	1705:1709	arg1	progression					1711:1721	tumor progression	1705:1721	tumor progression	1705:1721	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	5	68	theme	pressure	994:1001	arg1	source					1017:1022	a novel subatmospheric pressure (SubAP)/MALDI source	971:1022	a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	971:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	4	69	theme	matrix-assisted	779:793	arg1	spectrometry					828:839	matrix-assisted laser desorption/ionization mass spectrometry	779:839	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	779:859	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	5	70	theme	glycans	1122:1128	arg1	imaging					1102:1108	in situ imaging	1094:1108	in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1094:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	4	71	theme	past	668:671	arg1	decades					673:679	past decades	668:679	past decades	668:679	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	5	72	from	imaging	1102:1108	arg1	sections					1182:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	formalin-fixed paraffin-embedded (FFPE) tissue sections	1135:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	1	73	theme	various	184:190	arg1	glycoforms					192:201	various glycoforms	184:201	various glycoforms	184:201	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	0	74	theme	Subatmospheric	107:120	arg1	Source					142:147	a Novel Subatmospheric Pressure Ionization Source	99:147	a Novel Subatmospheric Pressure Ionization Source	99:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	0	75	theme	Ionization	131:140	arg1	Source					142:147	a Novel Subatmospheric Pressure Ionization Source	99:147	a Novel Subatmospheric Pressure Ionization Source	99:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	5	76	theme	Q	1031:1031	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	7	77	theme	mouse	1395:1399	arg1	section					1407:1413	a FFPE mouse brain section	1388:1413	a FFPE mouse brain section	1388:1413	A total of 55 N-glycans were successfully characterized and visualized from a FFPE mouse brain section.					
31507162	3	78	theme	disease-related	483:497	arg1	glycoforms					543:552	the glycoforms	539:552	especially the glycoforms	528:552	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	3	78	theme	disease-related	483:497	arg1	alteration					499:508	the disease-related alteration	479:508	the disease-related alteration of glycosylation	479:525	Hence, unraveling the disease-related alteration of glycosylation, especially the glycoforms, is crucial and beneficial to improving our understanding about the pathogenic mechanisms of various diseases.					
31507162	0	79	theme	Spectrometry	5:16	arg1	Imaging					18:24	Mass Spectrometry Imaging	0:24	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.	0:148	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	5	80	theme	SubAP	1004:1008	arg1	source					1017:1022	a novel subatmospheric pressure (SubAP)/MALDI source	971:1022	a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	971:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	4	81	theme	desorption/ionization	801:821	arg1	spectrometry					828:839	matrix-assisted laser desorption/ionization mass spectrometry	779:839	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	779:859	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	2	82	theme	patterns	417:424	arg1	changes					382:388	abnormal changes	373:388	abnormal changes of protein N-glycosylation patterns	373:424	It has been revealed that abnormal changes of protein N-glycosylation patterns are associated with many diseases.					
31507162	5	83	theme	/MALDI	1010:1015	arg1	source					1017:1022	a novel subatmospheric pressure (SubAP)/MALDI source	971:1022	a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	971:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	4	84	theme	spectrometry	828:839	arg1	MALDI-MSI					850:858	MALDI-MSI	850:858	MALDI-MSI	850:858	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	4	84	theme	spectrometry	828:839	arg1	imaging					841:847	matrix-assisted laser desorption/ionization mass spectrometry imaging	779:847	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	779:859	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	1	85	theme	biological	325:334	arg1	functions					336:344	biological functions	325:344	biological functions	325:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	9	86	theme	type	1682:1685	arg1	association					1662:1672	the potential association	1648:1672	the potential association of this type of N-glycans with tumor progression	1648:1721	High-mannose N-glycans exhibited elevated expression levels in the tumor region, indicating the potential association of this type of N-glycans with tumor progression.					
31507162	8	87	theme	spatial	1457:1463	arg1	patterns					1478:1485	different spatial distribution patterns	1447:1485	different spatial distribution patterns	1447:1485	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	2	88	theme	many	446:449	arg1	diseases					451:458	many diseases	446:458	many diseases	446:458	It has been revealed that abnormal changes of protein N-glycosylation patterns are associated with many diseases.					
31507162	0	89	theme	Paraffin-Embedded	59:75	arg1	Sections					84:91	Formalin-Fixed Paraffin-Embedded Tissue Sections	44:91	Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source	44:147	Mass Spectrometry Imaging of N-Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using a Novel Subatmospheric Pressure Ionization Source.					
31507162	5	90	theme	in	1094:1095	arg1	imaging					1102:1108	in situ imaging	1094:1108	in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections	1094:1189	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	8	91	theme	cancer	1533:1538	arg1	section					1547:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	a FFPE mouse ovarian cancer tissue section	1512:1553	Furthermore, 29 N-glycans with different spatial distribution patterns could be identified from a FFPE mouse ovarian cancer tissue section.					
31507162	4	92	dep	in	706:707	arg1	situ					709:712	situ	709:712	situ	709:712	In past decades, given the capability of in situ mapping of biomolecules and their region-specific localizations, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been widely applied to the discovery of potential biomarkers for many diseases.					
31507162	5	93	theme	HF	1042:1043	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	5	94	dep	paraffin-embedded	1150:1166	arg1	FFPE					1169:1172	FFPE	1169:1172	FFPE	1169:1172	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	1	95	theme	protein	242:248	arg1	PTMs					284:287	PTMs	284:287	PTMs	284:287	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	95	theme	protein	242:248	arg1	modifications					269:281	the most common and complex protein post-translational modifications	214:281	the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions	214:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	5	96	theme	quadrupole-orbitrap	1052:1070	arg1	spectrometer					1077:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer	1029:1088	In this study, we coupled a novel subatmospheric pressure (SubAP)/MALDI source with a Q Exactive HF hybrid quadrupole-orbitrap mass spectrometer for in situ imaging of N-linked glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections.					
31507162	2	97	theme	protein	393:399	arg1	patterns					417:424	protein N-glycosylation patterns	393:424	protein N-glycosylation patterns	393:424	It has been revealed that abnormal changes of protein N-glycosylation patterns are associated with many diseases.					
31507162	1	98	theme	modifications	269:281	arg1	one					207:209	one	207:209	one	207:209	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	98	theme	modifications	269:281	arg1	PTMs					284:287	PTMs	284:287	PTMs	284:287	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
31507162	1	98	theme	modifications	269:281	arg1	modifications					269:281	the most common and complex protein post-translational modifications	214:281	the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions	214:344	N-linked glycosylation, featuring various glycoforms, is one of the most common and complex protein post-translational modifications (PTMs) controlling protein structures and biological functions.					
30016589	2	0	theme	receptor	441:448	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	5	1	theme	resonance	1111:1119	arg1	spectroscopy					1121:1132	solution nuclear magnetic resonance spectroscopy	1085:1132	solution nuclear magnetic resonance spectroscopy	1085:1132	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	5	2	theme	resonance	1023:1031	arg1	frequencies					1033:1043	resonance frequencies	1023:1043	resonance frequencies	1023:1043	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	8	3	theme	glycan-glycan	1611:1623	arg1	contacts					1625:1632	intermolecular glycan-glycan contacts	1596:1632	intermolecular glycan-glycan contacts	1596:1632	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	7	4	theme	glycan-glycan	1489:1501	arg1	contacts					1503:1510	intermolecular glycan-glycan contacts	1474:1510	intermolecular glycan-glycan contacts previously proposed to stabilize this system	1474:1555	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	2	5	theme	γ	439:439	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	3	6	theme	IgG1	620:623	arg1	Fc					625:626	afucosylated IgG1 Fc	607:626	afucosylated IgG1 Fc	607:626	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	6	7	theme	±	1208:1208	arg1	penalty					1223:1229	the remaining 0.5 ± 0.1 kcal/mol penalty	1190:1229	the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a	1190:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	8	8	theme	limited	1654:1660	arg1	improvement					1662:1672	limited improvement	1654:1672	limited improvement	1654:1672	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	1	9	theme	cytotoxic	234:242	arg1	response					258:265	a cytotoxic cell-mediated response	232:265	a cytotoxic cell-mediated response	232:265	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	5	10	theme	spin	1057:1060	arg1	relaxation					1062:1071	nuclear spin relaxation	1049:1071	nuclear spin relaxation	1049:1071	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	4	11	theme	CD16a	800:804	arg1	Asn162-glycan					806:818	the CD16a Asn162-glycan	796:818	the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues	796:894	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	6	12	from	effects	1139:1145	arg1	Fc					1171:1172	IgG1 Fc	1166:1172	IgG1 Fc	1166:1172	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	1	13	theme	cell-mediated	244:256	arg1	response					258:265	a cytotoxic cell-mediated response	232:265	a cytotoxic cell-mediated response	232:265	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	2	14	theme	binding	463:469	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	3	15	theme	N-glycan	644:651	arg1	truncation					653:662	CD16a N-glycan truncation	638:662	CD16a N-glycan truncation	638:662	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	6	16	theme	remaining	1194:1202	arg1	penalty					1223:1229	the remaining 0.5 ± 0.1 kcal/mol penalty	1190:1229	the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a	1190:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	8	17	theme	intermolecular	1596:1609	arg1	contacts					1625:1632	intermolecular glycan-glycan contacts	1596:1632	intermolecular glycan-glycan contacts	1596:1632	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	7	18	theme	CD16a	1341:1345	arg1	Asn162-glycan					1347:1359	the CD16a Asn162-glycan	1337:1359	the CD16a Asn162-glycan	1337:1359	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	6	19	gly	fucosylated	1234:1244	arg1	CD16a					1262:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	7	20	theme	intermolecular	1474:1487	arg1	contacts					1503:1510	intermolecular glycan-glycan contacts	1474:1510	intermolecular glycan-glycan contacts previously proposed to stabilize this system	1474:1555	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	6	21	gly	fucosylation	1150:1161	arg1	IgG1					1166:1169	IgG1 Fc	1166:1172	IgG1 Fc	1166:1172	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	8	22	theme	conformational	1762:1775	arg1	heterogeneity					1777:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	6	23	theme	afucosylated	1293:1304	arg1	Fc					1311:1312	afucosylated IgG1 Fc	1293:1312	afucosylated IgG1 Fc	1293:1312	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	2	24	theme	CD16a	456:460	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	7	25	theme	direct	1452:1457	arg1	mechanism					1459:1467	a direct mechanism	1450:1467	a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system	1450:1555	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	4	26	theme	Tyr296	931:936	arg1	residue					938:944	the IgG Tyr296 residue	923:944	the IgG Tyr296 residue	923:944	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	7	27	theme	antibody	1375:1382	arg1	affinity					1384:1391	the antibody affinity	1371:1391	the antibody affinity	1371:1391	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	2	28	theme	-linked	359:365	arg1	carbohydrate					367:378	the asparagine (N)-linked carbohydrate	341:378	the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc)	341:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	3	29	theme	CD16a	638:642	arg1	truncation					653:662	CD16a N-glycan truncation	638:662	CD16a N-glycan truncation	638:662	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	3	30	theme	IgG1	512:515	arg1	fucosylation					520:531	IgG1 Fc fucosylation	512:531	IgG1 Fc fucosylation	512:531	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	1	31	theme	immunoglobulin	181:194	arg1	IgG1					200:203	IgG1	200:203	IgG1	200:203	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	1	31	theme	immunoglobulin	181:194	arg1	G1					196:197	immunoglobulin G1	181:197	the immunoglobulin G1 (IgG1) scaffold	177:213	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	0	32	theme	Antibody	0:7	arg1	Fucosylation					9:20	Antibody Fucosylation	0:20	Antibody Fucosylation	0:20	Antibody Fucosylation Lowers the FcγRIIIa/CD16a Affinity by Limiting the Conformations Sampled by the N162-Glycan.					
30016589	5	33	theme	nuclear	1094:1100	arg1	resonance					1111:1119	solution nuclear magnetic resonance	1085:1119	solution nuclear magnetic resonance spectroscopy	1085:1132	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	4	34	theme	conformations	760:772	arg1	manifold					748:755	manifold	748:755	manifold	748:755	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	2	35	theme	Core	294:297	arg1	modification					325:336	a prevalent modification	313:336	a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc)	313:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	35	theme	Core	294:297	arg1	fucosylation					299:310	Core fucosylation	294:310	Core fucosylation	294:310	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	5	36	theme	nuclear	1049:1055	arg1	relaxation					1062:1071	nuclear spin relaxation	1049:1071	nuclear spin relaxation	1049:1071	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	1	37	theme	G1	196:197	arg1	scaffold					206:213	the immunoglobulin G1 (IgG1) scaffold	177:213	the immunoglobulin G1 (IgG1) scaffold	177:213	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	5	38	theme	magnetic	1102:1109	arg1	resonance					1111:1119	solution nuclear magnetic resonance	1085:1119	solution nuclear magnetic resonance spectroscopy	1085:1132	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	6	39	theme	CD16a	1262:1266	arg1	penalty					1223:1229	the remaining 0.5 ± 0.1 kcal/mol penalty	1190:1229	the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a	1190:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	4	40	with	contacts	909:916	arg1	residue					938:944	the IgG Tyr296 residue	923:944	the IgG Tyr296 residue	923:944	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	2	41	theme	crystallizable	392:405	arg1	Fc					417:418	Fc	417:418	Fc	417:418	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	41	theme	crystallizable	392:405	arg1	fragment					407:414	the IgG1 crystallizable fragment	383:414	the IgG1 crystallizable fragment (Fc)	383:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	3	42	theme	Fc	517:518	arg1	fucosylation					520:531	IgG1 Fc fucosylation	512:531	IgG1 Fc fucosylation	512:531	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	1	43	theme	Fc	278:279	arg1	receptors					283:291	Fc γ receptors	278:291	Fc γ receptors	278:291	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	6	44	theme	binding	1254:1260	arg1	CD16a					1262:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	5	45	theme	Fc	983:984	arg1	structure					986:994	the IgG1 Fc structure	974:994	the IgG1 Fc structure	974:994	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	1	46	theme	Therapeutic	115:125	arg1	mAbs					150:153	mAbs	150:153	mAbs	150:153	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	1	46	theme	Therapeutic	115:125	arg1	antibodies					138:147	Therapeutic monoclonal antibodies	115:147	Therapeutic monoclonal antibodies (mAbs)	115:154	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	2	47	theme	IgG1	387:390	arg1	Fc					417:418	Fc	417:418	Fc	417:418	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	47	theme	IgG1	387:390	arg1	fragment					407:414	the IgG1 crystallizable fragment	383:414	the IgG1 crystallizable fragment (Fc)	383:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	48	theme	Fc	436:437	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	1	49	theme	γ	281:281	arg1	receptors					283:291	Fc γ receptors	278:291	Fc γ receptors	278:291	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	2	50	theme	mAb	484:486	arg1	efficacy					488:495	mAb efficacy	484:495	mAb efficacy	484:495	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	8	51	theme	antibody	1564:1571	arg1	engineering					1573:1583	antibody engineering	1564:1583	antibody engineering to enhance intermolecular glycan-glycan contacts	1564:1632	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	7	52	with	mechanism	1459:1467	arg1	contacts					1503:1510	intermolecular glycan-glycan contacts	1474:1510	intermolecular glycan-glycan contacts previously proposed to stabilize this system	1474:1555	Our results indicated the CD16a Asn162-glycan modulates the antibody affinity indirectly by reducing the volume sampled, as opposed to a direct mechanism with intermolecular glycan-glycan contacts previously proposed to stabilize this system.					
30016589	0	53	theme	FcγRIIIa/CD16a	33:46	arg1	Affinity					48:55	the FcγRIIIa/CD16a Affinity	29:55	the FcγRIIIa/CD16a Affinity	29:55	Antibody Fucosylation Lowers the FcγRIIIa/CD16a Affinity by Limiting the Conformations Sampled by the N162-Glycan.					
30016589	6	54	theme	IgG1	1306:1309	arg1	Fc					1311:1312	afucosylated IgG1 Fc	1293:1312	afucosylated IgG1 Fc	1293:1312	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	6	55	theme	IgG1	1246:1249	arg1	CD16a					1262:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	3	56	theme	0.1	569:571	arg1	±					567:567	±	567:567	±	567:567	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	3	57	theme	CD16a	545:549	arg1	affinity					551:558	the CD16a affinity	541:558	the CD16a affinity	541:558	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	5	58	theme	solution	1085:1092	arg1	resonance					1111:1119	solution nuclear magnetic resonance	1085:1119	solution nuclear magnetic resonance spectroscopy	1085:1132	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	4	59	theme	IgG	927:929	arg1	residue					938:944	the IgG Tyr296 residue	923:944	the IgG Tyr296 residue	923:944	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	6	60	theme	fucosylated	1234:1244	arg1	CD16a					1262:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	2	61	link	-linked	359:365	arg1	carbohydrate					367:378	the asparagine (N)-linked carbohydrate	341:378	the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc)	341:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	1	62	theme	monoclonal	127:136	arg1	mAbs					150:153	mAbs	150:153	mAbs	150:153	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	1	62	theme	monoclonal	127:136	arg1	antibodies					138:147	Therapeutic monoclonal antibodies	115:147	Therapeutic monoclonal antibodies (mAbs)	115:154	Therapeutic monoclonal antibodies (mAbs) are largely based on the immunoglobulin G1 (IgG1) scaffold, and many elicit a cytotoxic cell-mediated response by binding Fc γ receptors.					
30016589	6	63	theme	IgG1	1166:1169	arg1	Fc					1171:1172	IgG1 Fc	1166:1172	IgG1 Fc	1166:1172	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	4	64	theme	β-mannose	877:885	arg1	residues					887:894	the α-mannose(1-6)β-mannose residues	859:894	the α-mannose(1-6)β-mannose residues	859:894	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	2	65	from	carbohydrate	367:378	arg1	Fc					417:418	Fc	417:418	Fc	417:418	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	65	from	carbohydrate	367:378	arg1	fragment					407:414	the IgG1 crystallizable fragment	383:414	the IgG1 crystallizable fragment (Fc)	383:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	66	theme	prevalent	315:323	arg1	modification					325:336	a prevalent modification	313:336	a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc)	313:419	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	2	66	theme	prevalent	315:323	arg1	fucosylation					299:310	Core fucosylation	294:310	Core fucosylation	294:310	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	6	67	theme	fucosylation	1150:1161	arg1	effects					1139:1145	The effects	1135:1145	The effects of fucosylation on IgG1 Fc	1135:1172	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	3	68	theme	±	694:694	arg1	kcal/mol					700:707	1.2 ± 0.1 kcal/mol	690:707	1.2 ± 0.1 kcal/mol	690:707	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	3	69	theme	afucosylated	607:618	arg1	Fc					625:626	afucosylated IgG1 Fc	607:626	afucosylated IgG1 Fc	607:626	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	3	70	theme	1.7	563:565	arg1	±					567:567	±	567:567	±	567:567	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	4	71	theme	Fc	717:718	arg1	fucosylation					720:731	Fc fucosylation	717:731	Fc fucosylation	717:731	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30016589	3	72	gly	afucosylated	607:618	arg1	Fc					625:626	afucosylated IgG1 Fc	607:626	afucosylated IgG1 Fc	607:626	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	6	73	theme	kcal/mol	1214:1221	arg1	penalty					1223:1229	the remaining 0.5 ± 0.1 kcal/mol penalty	1190:1229	the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a	1190:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	8	74	theme	Asn162-glycan	1748:1760	arg1	heterogeneity					1777:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	8	75	theme	future	1679:1684	arg1	designs					1686:1692	future designs	1679:1692	future designs	1679:1692	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	6	76	theme	Fc	1251:1252	arg1	CD16a					1262:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	fucosylated IgG1 Fc binding CD16a	1234:1266	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	5	77	theme	IgG1	978:981	arg1	Fc					983:984	IgG1 Fc	978:984	the IgG1 Fc structure	974:994	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	8	78	theme	CD16a	1742:1746	arg1	heterogeneity					1777:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	the CD16a Asn162-glycan conformational heterogeneity	1738:1789	Thus, antibody engineering to enhance intermolecular glycan-glycan contacts will likely provide limited improvement, and future designs should maximize the affinity by maintaining the CD16a Asn162-glycan conformational heterogeneity.					
30016589	6	79	gly	afucosylated	1293:1304	arg1	Fc					1311:1312	afucosylated IgG1 Fc	1293:1312	afucosylated IgG1 Fc	1293:1312	The effects of fucosylation on IgG1 Fc may account for the remaining 0.5 ± 0.1 kcal/mol penalty of fucosylated IgG1 Fc binding CD16a when compared to that of afucosylated IgG1 Fc.					
30016589	2	80	theme	IIIa	450:453	arg1	affinity					471:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	the Fc γ receptor IIIa (CD16a) binding affinity	432:478	Core fucosylation, a prevalent modification to the asparagine (N)-linked carbohydrate on the IgG1 crystallizable fragment (Fc), decreases the Fc γ receptor IIIa (CD16a) binding affinity and mAb efficacy.					
30016589	5	81	from	changes	1012:1018	arg1	relaxation					1062:1071	nuclear spin relaxation	1049:1071	nuclear spin relaxation	1049:1071	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	5	81	from	changes	1012:1018	arg1	frequencies					1033:1043	resonance frequencies	1023:1043	resonance frequencies	1023:1043	Fucosylation also impacted the IgG1 Fc structure as indicated by changes in resonance frequencies and nuclear spin relaxation observed by solution nuclear magnetic resonance spectroscopy.					
30016589	3	82	dep	determined	501:510	arg1	reduced					533:539	reduced	533:539	determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc	501:626	We determined IgG1 Fc fucosylation reduced the CD16a affinity by 1.7 ± 0.1 kcal/mol when compared to that of afucosylated IgG1 Fc; however, CD16a N-glycan truncation decreased this penalty by 1.2 ± 0.1 kcal/mol or 70%.					
30016589	4	83	theme	α-mannose	863:871	arg1	residues					887:894	the α-mannose(1-6)β-mannose residues	859:894	the α-mannose(1-6)β-mannose residues	859:894	Fc fucosylation restricted the manifold of conformations sampled by displacing the CD16a Asn162-glycan that impinges upon the linkage between the α-mannose(1-6)β-mannose residues and promoted contacts with the IgG Tyr296 residue.					
30302011	7	0	theme	glycan	1169:1174	arg1	movements					1176:1184	glycan movements	1169:1184	glycan movements on a protomer	1169:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	1	from	loss	1125:1128	arg1	protomer					1191:1198	a protomer	1189:1198	a protomer	1189:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	3	2	from	antibodies	417:426	arg1	epitopes					390:397	CD4 binding site epitopes	373:397	CD4 binding site epitopes	373:397	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	3	2	from	antibodies	417:426	arg1	loop					364:367	V3 loop	361:367	V3 loop	361:367	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	1	3	theme	Env	129:131	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	3	theme	Env	129:131	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	4	theme	critical	206:213	arg1	candidate					223:231	a critical vaccine candidate	204:231	a critical vaccine candidate	204:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	0	5	theme	Trimer	74:79	arg1	Shield					46:51	Shield	46:51	Shield	46:51	Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan.					
30302011	4	6	theme	structural	488:497	arg1	rearrangements					499:512	the structural rearrangements	484:512	the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301	484:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	1	7	gly	glycoprotein	134:145	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	7	gly	glycoprotein	134:145	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	7	8	from	protomer	1191:1198	arg1	cascade					1158:1164	the cascade	1154:1164	the cascade of glycan movements on a protomer	1154:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	8	from	protomer	1191:1198	arg1	loss					1125:1128	the loss	1121:1128	the loss of a glycan	1121:1140	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	4	9	dep	glycan	560:565	arg1	shield					567:572	shield	567:572	shield after the loss of glycan N301	567:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	1	10	theme	vaccine	215:221	arg1	candidate					223:231	a critical vaccine candidate	204:231	a critical vaccine candidate	204:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	7	11	theme	antibody	1266:1273	arg1	epitope					1275:1281	an antibody epitope	1263:1281	an antibody epitope located at the edge of the diminishing effect	1263:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	5	12	theme	subtype	639:645	arg1	isolate					649:655	a naturally occurring subtype C isolate	617:655	a naturally occurring subtype C isolate	617:655	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	1	13	theme	candidate	223:231	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	13	theme	candidate	223:231	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	3	14	theme	glycan	341:346	arg1	N301					348:351	glycan N301	341:351	glycan N301	341:351	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	4	15	theme	molecular	442:450	arg1	dynamics					452:459	molecular dynamics	442:459	molecular dynamics techniques	442:470	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	5	16	theme	C	647:647	arg1	isolate					649:655	a naturally occurring subtype C isolate	617:655	a naturally occurring subtype C isolate	617:655	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	7	17	theme	point	1217:1221	arg1	mutation					1223:1230	the point mutation	1213:1230	the point mutation	1213:1230	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	18	theme	shield	1086:1091	arg1	ability					1095:1101	the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect	1075:1327	the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect	1075:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	3	19	theme	neutralising	404:415	arg1	antibodies					417:426	neutralising antibodies	404:426	neutralising antibodies	404:426	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	5	20	theme	underlying	760:769	arg1	epitopes					771:778	underlying epitopes	760:778	underlying epitopes	760:778	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	7	21	theme	epitope	1275:1281	arg1	integrity					1250:1258	the integrity	1246:1258	the integrity of an antibody epitope located at the edge of the diminishing effect	1246:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	3	22	theme	V3	361:362	arg1	loop					364:367	V3 loop	361:367	V3 loop	361:367	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	2	23	gly	glycosylated	258:269	arg1	Env					243:245	Env	243:245	Env	243:245	However, Env is densely glycosylated and thereby substantially protected from neutralisation.					
30302011	7	24	theme	movements	1176:1184	arg1	cascade					1158:1164	the cascade	1154:1164	the cascade of glycan movements on a protomer	1154:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	24	theme	movements	1176:1184	arg1	loss					1125:1128	the loss	1121:1128	the loss of a glycan	1121:1140	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	25	from	edge	1298:1301	arg1	located					1283:1289	located	1283:1289	located	1283:1289	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	0	26	theme	Structural	0:9	arg1	Rearrangements					11:24	Structural Rearrangements	0:24	Structural Rearrangements	0:24	Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan.					
30302011	7	27	theme	glycan	1079:1084	arg1	ability					1095:1101	the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect	1075:1327	the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect	1075:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	8	28	theme	glycan	1409:1414	arg1	research					1416:1423	HIV-1 Env glycan research	1399:1423	HIV-1 Env glycan research	1399:1423	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	1	29	theme	primary	154:160	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	29	theme	primary	154:160	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	7	30	theme	glycan	1135:1140	arg1	cascade					1158:1164	the cascade	1154:1164	the cascade of glycan movements on a protomer	1154:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	30	theme	glycan	1135:1140	arg1	loss					1125:1128	the loss	1121:1128	the loss of a glycan	1121:1140	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	4	31	theme	protective	532:541	arg1	qualities					543:551	the protective qualities	528:551	the protective qualities of the glycan shield after the loss of glycan N301	528:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	5	32	theme	increased	803:811	arg1	resistance					813:822	an increased resistance	800:822	an increased resistance to the VRC01 class of broadly neutralising antibodies	800:876	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	0	33	theme	Glycan	101:106	arg1	Loss					91:94	the Loss	87:94	the Loss of a Glycan	87:106	Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan.					
30302011	5	34	theme	occurring	629:637	arg1	isolate					649:655	a naturally occurring subtype C isolate	617:655	a naturally occurring subtype C isolate	617:655	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	5	35	theme	antibodies	867:876	arg1	class					837:841	the VRC01 class	827:841	the VRC01 class of broadly neutralising antibodies	827:876	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	3	36	theme	binding	377:383	arg1	epitopes					390:397	CD4 binding site epitopes	373:397	CD4 binding site epitopes	373:397	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	7	37	theme	diminishing	1310:1320	arg1	effect					1322:1327	the diminishing effect	1306:1327	the diminishing effect	1306:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	5	38	theme	neutralising	854:865	arg1	antibodies					867:876	broadly neutralising antibodies	846:876	broadly neutralising antibodies	846:876	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	3	39	theme	site	385:388	arg1	epitopes					390:397	CD4 binding site epitopes	373:397	CD4 binding site epitopes	373:397	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	4	40	theme	glycan	560:565	arg1	qualities					543:551	the protective qualities	528:551	the protective qualities of the glycan shield after the loss of glycan N301	528:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	7	41	from	cascade	1158:1164	arg1	protomer					1191:1198	a protomer	1189:1198	a protomer	1189:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	7	42	theme	effect	1322:1327	arg1	edge					1298:1301	the edge	1294:1301	the edge of the diminishing effect	1294:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	1	43	theme	humoral	176:182	arg1	response					191:198	the humoral immune response	172:198	the humoral immune response	172:198	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	8	44	dep	key	1352:1354	arg1	overlooked					1368:1377	overlooked	1368:1377	overlooked	1368:1377	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	6	45	theme	mutant	1026:1031	arg1	resistance					1008:1017	the neutralisation resistance	989:1017	the neutralisation resistance of the mutant	989:1031	Analysis of this mutant revealed several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant.					
30302011	4	46	dep	dynamics	452:459	arg1	techniques					461:470	techniques	461:470	molecular dynamics techniques	442:470	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	8	47	theme	related	1429:1435	arg1	studies					1445:1451	related vaccine studies	1429:1451	related vaccine studies	1429:1451	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	7	48	theme	detailed	1053:1060	arg1	insight					1062:1068	detailed insight	1053:1068	detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect	1053:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	3	49	theme	CD4	373:375	arg1	epitopes					390:397	CD4 binding site epitopes	373:397	CD4 binding site epitopes	373:397	Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies.					
30302011	8	50	theme	vaccine	1437:1443	arg1	studies					1445:1451	related vaccine studies	1429:1451	related vaccine studies	1429:1451	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	6	51	theme	mutant	896:901	arg1	Analysis					879:886	Analysis	879:886	Analysis of this mutant	879:901	Analysis of this mutant revealed several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant.					
30302011	8	52	theme	Env	1405:1407	arg1	research					1416:1423	HIV-1 Env glycan research	1399:1423	HIV-1 Env glycan research	1399:1423	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	8	53	theme	HIV-1	1399:1403	arg1	research					1416:1423	HIV-1 Env glycan research	1399:1423	HIV-1 Env glycan research	1399:1423	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	7	54	from	movements	1176:1184	arg1	protomer					1191:1198	a protomer	1189:1198	a protomer	1189:1198	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	5	55	theme	neutralising	726:737	arg1	antibodies					739:748	neutralising antibodies	726:748	neutralising antibodies targeting underlying epitopes	726:778	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	1	56	theme	immune	184:189	arg1	response					191:198	the humoral immune response	172:198	the humoral immune response	172:198	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	5	57	theme	N301A	665:669	arg1	mutant					671:676	its N301A mutant	661:676	its N301A mutant	661:676	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	8	58	theme	key	1352:1354	arg1	considerations					1380:1393	key, previously overlooked, considerations	1352:1393	key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies	1352:1451	These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.					
30302011	1	59	theme	HIV-1	113:117	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	59	theme	HIV-1	113:117	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	60	theme	response	191:198	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	60	theme	response	191:198	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	0	61	theme	Envelope	65:72	arg1	Trimer					74:79	an HIV-1 Envelope Trimer	56:79	an HIV-1 Envelope Trimer	56:79	Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan.					
30302011	5	62	theme	VRC01	831:835	arg1	class					837:841	the VRC01 class	827:841	the VRC01 class of broadly neutralising antibodies	827:876	We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies.					
30302011	7	63	theme	located	1283:1289	arg1	epitope					1275:1281	an antibody epitope	1263:1281	an antibody epitope located at the edge of the diminishing effect	1263:1327	These data provide detailed insight into the glycan shield's ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect.					
30302011	1	64	theme	envelope	119:126	arg1	glycoprotein					134:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein	109:145	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	1	64	theme	envelope	119:126	arg1	target					162:167	the primary target	150:167	the primary target of the humoral immune response and a critical vaccine candidate	150:231	The HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate.					
30302011	4	65	theme	N301	599:602	arg1	loss					584:587	the loss	580:587	the loss of glycan N301	580:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30302011	0	66	theme	HIV-1	59:63	arg1	Trimer					74:79	an HIV-1 Envelope Trimer	56:79	an HIV-1 Envelope Trimer	56:79	Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan.					
30302011	6	67	theme	several	912:918	arg1	glycans					920:926	several glycans	912:926	several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant	912:1031	Analysis of this mutant revealed several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant.					
30302011	6	68	theme	neutralisation	993:1006	arg1	resistance					1008:1017	the neutralisation resistance	989:1017	the neutralisation resistance of the mutant	989:1031	Analysis of this mutant revealed several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant.					
30302011	4	69	theme	glycan	592:597	arg1	N301					599:602	glycan N301	592:602	glycan N301	592:602	Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301.					
30602567	0	0	theme	jecorina	86:93	arg1	β-glucosidases					104:117	Hypocrea jecorina family 3 β-glucosidases	77:117	Hypocrea jecorina family 3 β-glucosidases	77:117	Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.					
30602567	7	1	theme	glucose	1308:1314	arg1	binding					1297:1303	binding	1297:1303	binding of glucose or cellobiose at the product site	1297:1348	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	5	2	theme	electrostatic	984:996	arg1	interactions					998:1009	relatively weak electrostatic interactions	968:1009	relatively weak electrostatic interactions with binding site residues	968:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	0	3	theme	Hypocrea	77:84	arg1	jecorina					86:93	Hypocrea jecorina	77:93	Hypocrea jecorina family 3 β-glucosidases	77:117	Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.					
30602567	10	4	theme	enzymatic	1808:1816	arg1	conversion					1826:1835	enzymatic biomass conversion	1808:1835	enzymatic biomass conversion	1808:1835	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	5	5	with	interactions	998:1009	arg1	residues					1029:1036	binding site residues	1016:1036	binding site residues	1016:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	5	6	contain	has	964:966	arg2	interactions					998:1009	relatively weak electrostatic interactions	968:1009	relatively weak electrostatic interactions with binding site residues	968:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	5	6	contain	has	964:966	arg1	cellobiose					953:962	cellobiose	953:962	cellobiose	953:962	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	1	7	theme	cellobiose	185:194	arg1	inhibition					196:205	cellobiose inhibition	185:205	cellobiose inhibition of endoglucanases and cellobiohydrolases	185:246	β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases.					
30602567	3	8	theme	processes	507:515	arg1	impact					487:492	the impact	483:492	the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	483:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	9	theme	molecular	758:766	arg1	simulations					782:792	molecular dynamics (MD) simulations	758:792	molecular dynamics (MD) simulations	758:792	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	7	10	from	site	1345:1348	arg1	binding					1297:1303	binding	1297:1303	binding of glucose or cellobiose at the product site	1297:1348	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	2	11	theme	glucose	339:345	arg1	concentrations					361:374	high glucose or cellobiose concentrations	334:374	high glucose or cellobiose concentrations	334:374	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	2	12	from	concentrations	361:374	arg1	transglycosylation					312:329	transglycosylation	312:329	transglycosylation	312:329	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	2	12	from	concentrations	361:374	arg1	inhibition					297:306	inhibition	297:306	inhibition	297:306	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	9	13	theme	aromatic	1534:1541	arg1	residues					1543:1550	these aromatic residues	1528:1550	these aromatic residues for mutation	1528:1563	Targeting these aromatic residues for mutation to reduce substrate affinity at the product site would therefore potentially mitigate transglycosidic activity.					
30602567	0	14	theme	family	95:100	arg1	β-glucosidases					104:117	Hypocrea jecorina family 3 β-glucosidases	77:117	Hypocrea jecorina family 3 β-glucosidases	77:117	Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.					
30602567	4	15	theme	higher	816:821	arg1	specificity					823:833	a 7-fold higher specificity	807:833	a 7-fold higher specificity for cellobiose	807:848	HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition.					
30602567	7	16	theme	product	1337:1343	arg1	site					1345:1348	the product site	1333:1348	the product site	1333:1348	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	2	17	theme	high	334:337	arg1	glucose					339:345	high glucose	334:345	high glucose	334:345	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	10	18	theme	β-glucosidases	1749:1762	arg1	variants					1698:1705	improved variants	1689:1705	improved variants of HjCel3A and other structurally similar β-glucosidases	1689:1762	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	8	19	theme	MD	1403:1404	arg1	simulations					1406:1416	MD simulations	1403:1416	MD simulations	1403:1416	MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443.					
30602567	2	20	gly	transglycosylation	312:329	arg2	concentrations					361:374	high glucose or cellobiose concentrations	334:374	high glucose or cellobiose concentrations	334:374	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	2	20	gly	transglycosylation	312:329	arg1	concentrations					361:374	high glucose or cellobiose concentrations	334:374	high glucose or cellobiose concentrations	334:374	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	10	21	theme	higher	1923:1928	arg1	loadings					1940:1947	higher substrate loadings	1923:1947	higher substrate loadings	1923:1947	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	6	22	theme	industrial	1166:1175	arg1	β-glucosidase					1177:1189	an industrial β-glucosidase	1163:1189	an industrial β-glucosidase despite its lower activity caused by transglycosylation	1163:1245	HjCel3A is, thus, preferable as an industrial β-glucosidase despite its lower activity caused by transglycosylation.					
30602567	3	23	theme	β-glucosidases	582:595	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	5	24	theme	site	1024:1027	arg1	residues					1029:1036	binding site residues	1016:1036	binding site residues	1016:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	2	25	theme	enzyme	452:457	arg1	mixtures					459:466	enzyme mixtures	452:466	enzyme mixtures	452:466	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	9	26	theme	substrate	1575:1583	arg1	affinity					1585:1592	substrate affinity	1575:1592	substrate affinity	1575:1592	Targeting these aromatic residues for mutation to reduce substrate affinity at the product site would therefore potentially mitigate transglycosidic activity.					
30602567	3	27	theme	MD	778:779	arg1	simulations					782:792	molecular dynamics (MD) simulations	758:792	molecular dynamics (MD) simulations	758:792	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	6	28	theme	lower	1203:1207	arg1	activity					1209:1216	its lower activity	1199:1216	its lower activity caused by transglycosylation	1199:1245	HjCel3A is, thus, preferable as an industrial β-glucosidase despite its lower activity caused by transglycosylation.					
30602567	8	29	theme	hydrophobic	1458:1468	arg1	interactions					1470:1481	hydrophobic interactions	1458:1481	hydrophobic interactions with Trp-37, Phe-260, and Tyr-443	1458:1515	MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443.					
30602567	10	30	theme	HjCel3A	1710:1716	arg1	variants					1698:1705	improved variants	1689:1705	improved variants of HjCel3A and other structurally similar β-glucosidases	1689:1762	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	9	31	theme	transglycosidic	1651:1665	arg1	activity					1667:1674	transglycosidic activity	1651:1674	transglycosidic activity	1651:1674	Targeting these aromatic residues for mutation to reduce substrate affinity at the product site would therefore potentially mitigate transglycosidic activity.					
30602567	10	32	theme	production	1859:1868	arg1	terms					1840:1844	terms	1840:1844	terms of yield and production cost	1840:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	7	33	theme	intermediate	1389:1400	arg1	formation					1356:1364	formation	1356:1364	formation of the glycosyl-enzyme intermediate	1356:1400	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	3	34	from	activity	535:542	arg1	HjCel3A					621:627	HjCel3A	621:627	HjCel3A	621:627	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	34	from	activity	535:542	arg1	jecorina					611:618	Hypocrea jecorina	602:618	Hypocrea jecorina	602:618	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	34	from	activity	535:542	arg1	HjCel3B					633:639	HjCel3B	633:639	HjCel3B	633:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	10	35	theme	similar	1741:1747	arg1	β-glucosidases					1749:1762	other structurally similar β-glucosidases	1722:1762	other structurally similar β-glucosidases	1722:1762	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	1	36	theme	endoglucanases	210:223	arg1	inhibition					196:205	cellobiose inhibition	185:205	cellobiose inhibition of endoglucanases and cellobiohydrolases	185:246	β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases.					
30602567	0	37	theme	Kinetic	0:6	arg1	dynamics					22:29	Kinetic and molecular dynamics	0:29	Kinetic and molecular dynamics	0:29	Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.					
30602567	10	38	theme	yield	1849:1853	arg1	terms					1840:1844	terms	1840:1844	terms of yield and production cost	1840:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	10	39	dep	yield	1849:1853	arg1	cost					1870:1873	cost	1870:1873	cost	1870:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	2	40	theme	overall	430:436	arg1	efficiency					438:447	the overall efficiency	426:447	the overall efficiency of enzyme mixtures	426:466	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	7	41	theme	competing	1253:1261	arg1	pathway					1263:1269	This competing pathway	1248:1269	This competing pathway to hydrolysis	1248:1283	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	4	42	theme	greater	867:873	arg1	tendency					875:882	greater tendency	867:882	greater tendency for glucose inhibition	867:905	HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition.					
30602567	2	43	theme	cellobiose	350:359	arg1	concentrations					361:374	high glucose or cellobiose concentrations	334:374	high glucose or cellobiose concentrations	334:374	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	1	44	theme	cellobiohydrolases	229:246	arg1	inhibition					196:205	cellobiose inhibition	185:205	cellobiose inhibition of endoglucanases and cellobiohydrolases	185:246	β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases.					
30602567	0	45	theme	molecular	12:20	arg1	dynamics					22:29	Kinetic and molecular dynamics	0:29	Kinetic and molecular dynamics	0:29	Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.					
30602567	3	46	theme	relevant	564:571	arg1	β-glucosidases					582:595	the industrially relevant family 3 β-glucosidases	547:595	the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	547:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	5	47	theme	Energy	908:913	arg1	analysis					929:936	Energy decomposition analysis	908:936	Energy decomposition analysis	908:936	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	10	48	theme	economic	1789:1796	arg1	effect					1798:1803	a significant economic effect	1775:1803	a significant economic effect on enzymatic biomass conversion in terms of yield and production cost	1775:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	4	49	contain	had	803:805	arg1	HjCel3B					795:801	HjCel3B	795:801	HjCel3B	795:801	HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition.					
30602567	4	49	contain	had	803:805	arg2	specificity					823:833	a 7-fold higher specificity	807:833	a 7-fold higher specificity for cellobiose	807:848	HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition.					
30602567	3	50	theme	binding	720:726	arg1	calculations					740:751	binding free energy calculations	720:751	binding free energy calculations	720:751	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	5	51	theme	decomposition	915:927	arg1	analysis					929:936	Energy decomposition analysis	908:936	Energy decomposition analysis	908:936	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	10	52	theme	biomass	1818:1824	arg1	conversion					1826:1835	enzymatic biomass conversion	1808:1835	enzymatic biomass conversion	1808:1835	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	10	53	theme	significant	1777:1787	arg1	effect					1798:1803	a significant economic effect	1775:1803	a significant economic effect on enzymatic biomass conversion in terms of yield and production cost	1775:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	3	54	theme	kinetic	703:709	arg1	studies					711:717	kinetic studies	703:717	kinetic studies	703:717	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	5	55	theme	other	1105:1109	arg1	enzymes					1122:1128	other hydrolytic enzymes	1105:1128	other hydrolytic enzymes	1105:1128	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	2	56	theme	mixtures	459:466	arg1	activity					398:405	their activity	392:405	their activity	392:405	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	2	56	theme	mixtures	459:466	arg1	efficiency					438:447	the overall efficiency	426:447	the overall efficiency of enzyme mixtures	426:466	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	3	57	from	jecorina	611:618	arg1	β-glucosidases					582:595	the industrially relevant family 3 β-glucosidases	547:595	the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	547:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	57	from	jecorina	611:618	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	10	58	theme	other	1722:1726	arg1	β-glucosidases					1749:1762	other structurally similar β-glucosidases	1722:1762	other structurally similar β-glucosidases	1722:1762	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	5	59	theme	hydrolytic	1111:1120	arg1	enzymes					1122:1128	other hydrolytic enzymes	1105:1128	other hydrolytic enzymes	1105:1128	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	10	60	from	effect	1798:1803	arg1	terms					1840:1844	terms	1840:1844	terms of yield and production cost	1840:1873	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	10	60	from	effect	1798:1803	arg1	conversion					1826:1835	enzymatic biomass conversion	1808:1835	enzymatic biomass conversion	1808:1835	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	9	61	theme	product	1601:1607	arg1	site					1609:1612	the product site	1597:1612	the product site	1597:1612	Targeting these aromatic residues for mutation to reduce substrate affinity at the product site would therefore potentially mitigate transglycosidic activity.					
30602567	4	62	theme	glucose	888:894	arg1	inhibition					896:905	glucose inhibition	888:905	glucose inhibition	888:905	HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition.					
30602567	5	63	theme	binding	1016:1022	arg1	residues					1029:1036	binding site residues	1016:1036	binding site residues	1016:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	10	64	theme	substrate	1930:1938	arg1	loadings					1940:1947	higher substrate loadings	1923:1947	higher substrate loadings	1923:1947	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	3	65	theme	molecular	674:682	arg1	mechanisms					684:693	the underlying molecular mechanisms	659:693	the underlying molecular mechanisms	659:693	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	1	66	theme	enzymatic	143:151	arg1	conversion					161:170	enzymatic biomass conversion	143:170	enzymatic biomass conversion	143:170	β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases.					
30602567	3	67	from	HjCel3A	621:627	arg1	β-glucosidases					582:595	the industrially relevant family 3 β-glucosidases	547:595	the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	547:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	67	from	HjCel3A	621:627	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	8	68	with	interactions	1470:1481	arg1	Tyr-443					1509:1515	Tyr-443	1509:1515	Tyr-443	1509:1515	MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443.					
30602567	8	68	with	interactions	1470:1481	arg1	Trp-37					1488:1493	Trp-37	1488:1493	Trp-37	1488:1493	MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443.					
30602567	8	68	with	interactions	1470:1481	arg1	Phe-260					1496:1502	Phe-260	1496:1502	Phe-260	1496:1502	MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443.					
30602567	3	69	theme	free	728:731	arg1	calculations					740:751	binding free energy calculations	720:751	binding free energy calculations	720:751	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	70	theme	Hypocrea	602:609	arg1	jecorina					611:618	Hypocrea jecorina	602:618	Hypocrea jecorina	602:618	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	1	71	theme	biomass	153:159	arg1	conversion					161:170	enzymatic biomass conversion	143:170	enzymatic biomass conversion	143:170	β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases.					
30602567	3	72	theme	hydrolytic	524:533	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	7	73	theme	cellobiose	1319:1328	arg1	binding					1297:1303	binding	1297:1303	binding of glucose or cellobiose at the product site	1297:1348	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30602567	3	74	theme	energy	733:738	arg1	calculations					740:751	binding free energy calculations	720:751	binding free energy calculations	720:751	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	75	theme	underlying	663:672	arg1	mechanisms					684:693	the underlying molecular mechanisms	659:693	the underlying molecular mechanisms	659:693	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	76	from	impact	487:492	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	77	theme	family	573:578	arg1	β-glucosidases					582:595	the industrially relevant family 3 β-glucosidases	547:595	the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	547:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	78	from	HjCel3B	633:639	arg1	β-glucosidases					582:595	the industrially relevant family 3 β-glucosidases	547:595	the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	547:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	3	78	from	HjCel3B	633:639	arg1	activity					535:542	the hydrolytic activity	520:542	the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B	520:639	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	10	79	theme	improved	1689:1696	arg1	variants					1698:1705	improved variants	1689:1705	improved variants of HjCel3A and other structurally similar β-glucosidases	1689:1762	Engineering improved variants of HjCel3A and other structurally similar β-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings.					
30602567	3	80	theme	dynamics	768:775	arg1	simulations					782:792	molecular dynamics (MD) simulations	758:792	molecular dynamics (MD) simulations	758:792	We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 β-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations.					
30602567	2	81	theme	enzymes	286:292	arg1	susceptibility					262:275	the susceptibility	258:275	the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations	258:374	However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures.					
30602567	5	82	theme	weak	979:982	arg1	interactions					998:1009	relatively weak electrostatic interactions	968:1009	relatively weak electrostatic interactions with binding site residues	968:1036	Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes.					
30602567	7	83	theme	glycosyl-enzyme	1373:1387	arg1	intermediate					1389:1400	the glycosyl-enzyme intermediate	1369:1400	the glycosyl-enzyme intermediate	1369:1400	This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate.					
30407002	5	0	theme	proximity-induced	780:796	arg1	hybridization					798:810	the proximity-induced hybridization	776:810	the proximity-induced hybridization between two probes	776:829	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	4	1	theme	target	705:710	arg1	proteins					712:719	target proteins	705:719	target proteins	705:719	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	5	2	dep	leads	845:849	arg1	followed					909:916	followed	909:916	followed by triggering of HCR assembly	909:946	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	2	3	from	glycans	325:331	arg1	protein					346:352	a certain protein	336:352	a certain protein	336:352	However, the ubiquity of glycosylation makes probing the glycans on a certain protein as difficult as looking for a needle in a haystack.					
30407002	4	4	theme	probes	606:611	arg1	kinds					593:597	two kinds	589:597	two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively	589:733	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	0	5	theme	Chain	107:111	arg1	Reaction					113:120	Proximity-Induced Hybridization Chain Reaction	75:120	Proximity-Induced Hybridization Chain Reaction	75:120	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	0	6	from	Visualization	10:22	arg1	Zebrafish					61:69	Zebrafish	61:69	Zebrafish	61:69	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	7	7	theme	protein-specific	1170:1185	arg1	glycosylation					1187:1199	protein-specific glycosylation	1170:1199	protein-specific glycosylation	1170:1199	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	3	8	theme	protein-specific	524:539	arg1	glycosylation					541:553	protein-specific glycosylation	524:553	protein-specific glycosylation	524:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	4	9	theme	DNA	602:604	arg1	probes					606:611	DNA probes	602:611	DNA probes	602:611	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	7	10	theme	disease	1264:1270	arg1	process					1272:1278	disease process	1264:1278	disease process	1264:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	7	10	theme	disease	1264:1270	arg1	glycans					1246:1252	dynamic glycans state and disease process	1238:1278	dynamic glycans state and disease process	1238:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	4	11	theme	glycan	614:619	arg1	probes					632:637	glycan conversion probes	614:637	glycan conversion probes	614:637	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	7	12	dep	glycans	1246:1252	arg1	state					1254:1258	state	1254:1258	state	1254:1258	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	7	12	dep	glycans	1246:1252	arg1	process					1272:1278	disease process	1264:1278	disease process	1264:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	7	12	dep	glycans	1246:1252	arg1	glycans					1246:1252	dynamic glycans state and disease process	1238:1278	dynamic glycans state and disease process	1238:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	5	13	theme	recognition	889:899	arg1	probes					901:906	protein recognition probes	881:906	protein recognition probes	881:906	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	3	14	theme	amplified	497:505	arg1	visualization					507:519	amplified visualization	497:519	amplified visualization of protein-specific glycosylation	497:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	4	15	theme	recognition	651:661	arg1	probes					663:668	protein recognition probes	643:668	protein recognition probes	643:668	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	3	16	theme	hybridization	449:461	arg1	HCR					479:481	HCR	479:481	HCR	479:481	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	3	16	theme	hybridization	449:461	arg1	reaction					469:476	proximity-induced hybridization chain reaction	431:476	a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation	429:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	6	17	theme	CEM	1047:1049	arg1	cells					1051:1055	living CEM cells	1040:1055	living CEM cells	1040:1055	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	2	18	theme	glycosylation	293:305	arg1	ubiquity					281:288	the ubiquity	277:288	the ubiquity of glycosylation	277:305	However, the ubiquity of glycosylation makes probing the glycans on a certain protein as difficult as looking for a needle in a haystack.					
30407002	1	19	theme	great	200:204	arg1	importance					206:215	great importance	200:215	great importance	200:215	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	7	20	theme	dynamic	1238:1244	arg1	state					1254:1258	state	1254:1258	state	1254:1258	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	7	20	theme	dynamic	1238:1244	arg1	process					1272:1278	disease process	1264:1278	disease process	1264:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	7	20	theme	dynamic	1238:1244	arg1	glycans					1246:1252	dynamic glycans state and disease process	1238:1278	dynamic glycans state and disease process	1238:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	2	21	theme	certain	338:344	arg1	protein					346:352	a certain protein	336:352	a certain protein	336:352	However, the ubiquity of glycosylation makes probing the glycans on a certain protein as difficult as looking for a needle in a haystack.					
30407002	6	22	theme	drug	1107:1110	arg1	treatment					1112:1120	drug treatment	1107:1120	drug treatment	1107:1120	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	6	23	theme	sialic	1025:1030	arg1	acid					1032:1035	tyrosine-protein kinase 7-specific sialic acid	990:1035	tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish	990:1069	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	2	24	from	needle	384:389	arg1	haystack					396:403	a haystack	394:403	a haystack	394:403	However, the ubiquity of glycosylation makes probing the glycans on a certain protein as difficult as looking for a needle in a haystack.					
30407002	5	25	theme	probes	901:906	arg1	structure-switching					858:876	the structure-switching	854:876	the structure-switching of protein recognition probes	854:906	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	3	26	theme	proximity-induced	431:447	arg1	HCR					479:481	HCR	479:481	HCR	479:481	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	3	26	theme	proximity-induced	431:447	arg1	reaction					469:476	proximity-induced hybridization chain reaction	431:476	a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation	429:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	0	27	theme	Protein-Specific	27:42	arg1	Glycosylation					44:56	Protein-Specific Glycosylation	27:56	Protein-Specific Glycosylation	27:56	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	6	28	theme	kinase 7-specific	1007:1023	arg1	acid					1032:1035	tyrosine-protein kinase 7-specific sialic acid	990:1035	tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish	990:1069	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	7	29	theme	potential	1137:1145	arg1	tool					1147:1150	a potential tool	1135:1150	a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process	1135:1278	It provides a potential tool for investigating protein-specific glycosylation and researching the relation between dynamic glycans state and disease process.					
30407002	4	30	theme	probes	663:668	arg1	kinds					593:597	two kinds	589:597	two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively	589:733	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	6	31	theme	tyrosine-protein	990:1005	arg1	acid					1032:1035	tyrosine-protein kinase 7-specific sialic acid	990:1035	tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish	990:1069	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	1	32	gly	glycosylation	144:156	arg1	proteins					177:184	specific proteins	168:184	specific proteins	168:184	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	5	33	theme	HCR	935:937	arg1	assembly					939:946	HCR assembly	935:946	HCR assembly	935:946	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	1	34	theme	proteins	177:184	arg1	states					158:163	glycosylation states	144:163	glycosylation states of specific proteins	144:184	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	6	35	used	used	972:975	arg2	strategy					954:961	This strategy	949:961	This strategy	949:961	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	4	36	attach	attached	681:688	arg1	proteins					712:719	target proteins	705:719	target proteins	705:719	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	4	36	attach	attached	681:688	arg2	probes					632:637	glycan conversion probes	614:637	glycan conversion probes	614:637	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	4	36	attach	attached	681:688	arg2	probes					606:611	DNA probes	602:611	DNA probes	602:611	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	4	36	attach	attached	681:688	arg2	probes					663:668	protein recognition probes	643:668	protein recognition probes	643:668	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	4	36	attach	attached	681:688	arg1	glycans					693:699	glycans	693:699	glycans	693:699	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	3	37	theme	reaction	469:476	arg1	strategy					484:491	a proximity-induced hybridization chain reaction (HCR) strategy	429:491	a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation	429:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	0	38	theme	Glycosylation	44:56	arg1	Visualization					10:22	Visualization	10:22	Visualization of Protein-Specific Glycosylation in Zebrafish	10:69	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	5	39	theme	sequential	741:750	arg1	binding					752:758	sequential binding	741:758	sequential binding to the targets	741:773	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	6	40	theme	living	1040:1045	arg1	cells					1051:1055	living CEM cells	1040:1055	living CEM cells	1040:1055	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	1	41	from	roles	238:242	arg1	development					255:265	disease development	247:265	disease development	247:265	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	5	42	theme	protein	881:887	arg1	probes					901:906	protein recognition probes	881:906	protein recognition probes	881:906	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	1	43	theme	glycosylation	144:156	arg1	states					158:163	glycosylation states	144:163	glycosylation states of specific proteins	144:184	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	6	44	from	acid	1032:1035	arg1	zebrafish					1061:1069	zebrafish	1061:1069	zebrafish	1061:1069	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	6	44	from	acid	1032:1035	arg1	cells					1051:1055	living CEM cells	1040:1055	living CEM cells	1040:1055	This strategy has been used to visualize tyrosine-protein kinase 7-specific sialic acid in living CEM cells and zebrafish and to monitor its variation during drug treatment.					
30407002	1	45	theme	states	158:163	arg1	visualization					127:139	The visualization	123:139	The visualization of glycosylation states of specific proteins in vivo	123:192	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	1	46	theme	disease	247:253	arg1	development					255:265	disease development	247:265	disease development	247:265	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	5	47	theme	assembly	939:946	arg1	triggering					921:930	triggering	921:930	triggering of HCR assembly	921:946	Upon sequential binding to the targets, the proximity-induced hybridization between two probes occurs, which leads to the structure-switching of protein recognition probes, followed by triggering of HCR assembly.					
30407002	1	48	theme	specific	168:175	arg1	proteins					177:184	specific proteins	168:184	specific proteins	168:184	The visualization of glycosylation states of specific proteins in vivo is of great importance for uncovering their roles in disease development.					
30407002	4	49	theme	conversion	621:630	arg1	probes					632:637	glycan conversion probes	614:637	glycan conversion probes	614:637	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	0	50	theme	Hybridization	93:105	arg1	Reaction					113:120	Proximity-Induced Hybridization Chain Reaction	75:120	Proximity-Induced Hybridization Chain Reaction	75:120	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	3	51	theme	glycosylation	541:553	arg1	visualization					507:519	amplified visualization	497:519	amplified visualization of protein-specific glycosylation	497:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	3	52	theme	chain	463:467	arg1	HCR					479:481	HCR	479:481	HCR	479:481	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	3	52	theme	chain	463:467	arg1	reaction					469:476	proximity-induced hybridization chain reaction	431:476	a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation	429:553	Herein, we demonstrate a proximity-induced hybridization chain reaction (HCR) strategy for amplified visualization of protein-specific glycosylation.					
30407002	4	53	theme	protein	643:649	arg1	probes					663:668	protein recognition probes	643:668	protein recognition probes	643:668	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
30407002	0	54	theme	Proximity-Induced	75:91	arg1	Reaction					113:120	Proximity-Induced Hybridization Chain Reaction	75:120	Proximity-Induced Hybridization Chain Reaction	75:120	Amplified Visualization of Protein-Specific Glycosylation in Zebrafish via Proximity-Induced Hybridization Chain Reaction.					
30407002	4	55	theme	probes	632:637	arg1	kinds					593:597	two kinds	589:597	two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively	589:733	The strategy relies on designing two kinds of DNA probes, glycan conversion probes and protein recognition probes, which are attached to glycans and target proteins, respectively.					
31423004	5	0	theme	tetra-antennary	774:788	arg1	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	3	1	theme	recombinant	507:517	arg1	rhAT					529:532	rhAT	529:532	rhAT	529:532	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	3	1	theme	recombinant	507:517	arg1	AT					525:526	recombinant human AT	507:526	recombinant human AT (rhAT) with core-fucosylated oligosaccharides	507:572	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	1	2	theme	glycosylation	240:252	arg1	sites					254:258	all four possible glycosylation sites	222:258	all four possible glycosylation sites	222:258	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	5	3	theme	complex	790:796	arg1	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	4	dep	type	798:801	arg1	species					847:853	species	847:853	species	847:853	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	3	5	theme	human	519:523	arg1	rhAT					529:532	rhAT	529:532	rhAT	529:532	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	3	5	theme	human	519:523	arg1	AT					525:526	recombinant human AT	507:526	recombinant human AT (rhAT) with core-fucosylated oligosaccharides	507:572	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	5	6	theme	variable	738:745	arg1	structures					754:763	more variable glycan structures	733:763	more variable glycan structures	733:763	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	6	theme	variable	738:745	arg1	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	6	theme	variable	738:745	arg1	components					864:873	minor components	858:873	minor components compared to plasma-derived human AT (phAT)	858:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	6	theme	variable	738:745	arg1	type					817:820	high-mannose type	804:820	high-mannose type	804:820	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	6	theme	variable	738:745	arg1	6-phosphate					835:845	mannose 6-phosphate	827:845	mannose 6-phosphate	827:845	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	3	7	with	AT	525:526	arg1	oligosaccharides					557:572	core-fucosylated oligosaccharides	540:572	core-fucosylated oligosaccharides	540:572	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	4	8	theme	FUT8	681:684	arg1	cells					699:703	FUT8 knockout CHO cells	681:703	FUT8 knockout CHO cells in production	681:717	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	5	9	theme	glycan	747:752	arg1	structures					754:763	more variable glycan structures	733:763	more variable glycan structures	733:763	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	9	theme	glycan	747:752	arg1	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	9	theme	glycan	747:752	arg1	components					864:873	minor components	858:873	minor components compared to plasma-derived human AT (phAT)	858:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	9	theme	glycan	747:752	arg1	type					817:820	high-mannose type	804:820	high-mannose type	804:820	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	9	theme	glycan	747:752	arg1	6-phosphate					835:845	mannose 6-phosphate	827:845	mannose 6-phosphate	827:845	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	1	10	from	oligosaccharide	303:317	arg1	Asn135					322:327	Asn135	322:327	Asn135	322:327	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	3	11	theme	core-fucosylated	540:555	arg1	oligosaccharides					557:572	core-fucosylated oligosaccharides	540:572	core-fucosylated oligosaccharides	540:572	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	4	12	theme	core-fucose	597:607	arg1	oligosaccharides					609:624	core-fucose oligosaccharides	597:624	core-fucose oligosaccharides	597:624	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	1	13	contain	has	154:156	arg1	antithrombin					136:147	Human antithrombin	130:147	Human antithrombin (AT)	130:152	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	13	contain	has	154:156	arg2	isoforms					162:169	two isoforms	158:169	two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135	158:327	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	13	contain	has	154:156	arg1	AT					150:151	AT	150:151	AT	150:151	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	5	14	link	plasma-derived	887:900	arg1	phAT					912:915	phAT	912:915	phAT	912:915	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	14	link	plasma-derived	887:900	arg1	AT					908:909	plasma-derived human AT	887:909	plasma-derived human AT (phAT)	887:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	4	15	theme	POTELLIGENT®	645:656	arg1	technology					658:667	POTELLIGENT® technology	645:667	POTELLIGENT® technology	645:667	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	0	16	dep	Using	99:103	arg1	Recombinant					87:97	Recombinant	87:97	Recombinant	87:97	A Comparison of the Oligosaccharide Structures of Antithrombin Derived from Plasma and Recombinant Using POTELLIGENT® Technology.					
31423004	5	17	theme	plasma-derived	887:900	arg1	phAT					912:915	phAT	912:915	phAT	912:915	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	17	theme	plasma-derived	887:900	arg1	AT					908:909	plasma-derived human AT	887:909	plasma-derived human AT (phAT)	887:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	2	18	theme	complex-type	401:412	arg1	oligosaccharides					414:429	biantennary complex-type oligosaccharides	389:429	biantennary complex-type oligosaccharides lacking a core fucose	389:451	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	0	19	theme	POTELLIGENT®	105:116	arg1	Technology					118:127	POTELLIGENT® Technology	105:127	POTELLIGENT® Technology	105:127	A Comparison of the Oligosaccharide Structures of Antithrombin Derived from Plasma and Recombinant Using POTELLIGENT® Technology.					
31423004	7	20	theme	oligosaccharide	1198:1212	arg1	profiles					1214:1221	site-specific oligosaccharide profiles	1184:1221	site-specific oligosaccharide profiles	1184:1221	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	2	21	theme	biantennary	389:399	arg1	oligosaccharides					414:429	biantennary complex-type oligosaccharides	389:429	biantennary complex-type oligosaccharides lacking a core fucose	389:451	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	7	22	theme	oligosaccharide	1156:1170	arg1	structures					1172:1181	their respective oligosaccharide structures	1139:1181	their respective oligosaccharide structures	1139:1181	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	6	23	from	addition	922:929	arg1	different					969:977	different	969:977	different	969:977	In addition, the site-specific glycan profile was different between two ATs.					
31423004	6	23	from	addition	922:929	arg1	profile					957:963	the site-specific glycan profile	932:963	the site-specific glycan profile	932:963	In addition, the site-specific glycan profile was different between two ATs.					
31423004	1	24	gly	glycosylated	206:217	arg1	α-form					196:201	the predominant α-form	180:201	the predominant α-form	180:201	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	24	gly	glycosylated	206:217	arg2	sites					254:258	all four possible glycosylation sites	222:258	all four possible glycosylation sites	222:258	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	24	gly	glycosylated	206:217	arg2	α-form					196:201	the predominant α-form	180:201	the predominant α-form	180:201	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	24	gly	glycosylated	206:217	arg1	sites					254:258	all four possible glycosylation sites	222:258	all four possible glycosylation sites	222:258	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	25	gly	glycosylation	240:252	arg2	sites					254:258	all four possible glycosylation sites	222:258	all four possible glycosylation sites	222:258	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	25	gly	glycosylation	240:252	arg2	four					226:229	four	226:229	four	226:229	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	4	26	theme	CHO	695:697	arg1	cells					699:703	FUT8 knockout CHO cells	681:703	FUT8 knockout CHO cells in production	681:717	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	7	27	theme	structures	1172:1181	arg1	terms					1130:1134	terms	1130:1134	terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1130:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	28	theme	rhAT	1088:1091	arg1	comparison					1074:1083	a comparison	1072:1083	a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1072:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	4	29	theme	knockout	686:693	arg1	cells					699:703	FUT8 knockout CHO cells	681:703	FUT8 knockout CHO cells in production	681:717	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	5	30	theme	mannose	827:833	arg1	6-phosphate					835:845	mannose 6-phosphate	827:845	mannose 6-phosphate	827:845	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	1	31	theme	abundant	274:281	arg1	β-isoform					283:291	the lower abundant β-isoform	264:291	the lower abundant β-isoform	264:291	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	6	32	from	different	969:977	arg1	addition					922:929	addition	922:929	addition	922:929	In addition, the site-specific glycan profile was different between two ATs.					
31423004	2	33	theme	core	441:444	arg1	fucose					446:451	a core fucose	439:451	a core fucose	439:451	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	3	34	theme	ovary	481:485	arg1	cells					493:497	Chinese hamster ovary (CHO) cells	465:497	Chinese hamster ovary (CHO) cells	465:497	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	7	35	theme	α-	1241:1242	arg1	profiles					1214:1221	site-specific oligosaccharide profiles	1184:1221	site-specific oligosaccharide profiles	1184:1221	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	35	theme	α-	1241:1242	arg1	ratio					1232:1236	the ratio	1228:1236	the ratio of α- and β-forms	1228:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	35	theme	α-	1241:1242	arg1	structures					1172:1181	their respective oligosaccharide structures	1139:1181	their respective oligosaccharide structures	1139:1181	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	2	36	theme	AT	374:375	arg1	structure					355:363	The main oligosaccharide structure	330:363	The main oligosaccharide structure of human AT	330:375	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	0	37	theme	Structures	36:45	arg1	Comparison					2:11	A Comparison	0:11	A Comparison of the Oligosaccharide Structures of Antithrombin	0:61	A Comparison of the Oligosaccharide Structures of Antithrombin Derived from Plasma and Recombinant Using POTELLIGENT® Technology.					
31423004	2	38	theme	main	334:337	arg1	structure					355:363	The main oligosaccharide structure	330:363	The main oligosaccharide structure of human AT	330:375	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	7	39	from	effect	1013:1018	arg1	efficacy					1043:1050	efficacy	1043:1050	efficacy	1043:1050	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	39	from	effect	1013:1018	arg1	safety					1056:1061	safety	1056:1061	safety	1056:1061	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	5	40	theme	minor	858:862	arg1	structures					754:763	more variable glycan structures	733:763	more variable glycan structures	733:763	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	40	theme	minor	858:862	arg1	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	40	theme	minor	858:862	arg1	components					864:873	minor components	858:873	minor components compared to plasma-derived human AT (phAT)	858:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	40	theme	minor	858:862	arg1	type					817:820	high-mannose type	804:820	high-mannose type	804:820	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	40	theme	minor	858:862	arg1	6-phosphate					835:845	mannose 6-phosphate	827:845	mannose 6-phosphate	827:845	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	41	theme	human	902:906	arg1	phAT					912:915	phAT	912:915	phAT	912:915	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	41	theme	human	902:906	arg1	AT					908:909	plasma-derived human AT	887:909	plasma-derived human AT (phAT)	887:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	3	42	theme	Chinese	465:471	arg1	cells					493:497	Chinese hamster ovary (CHO) cells	465:497	Chinese hamster ovary (CHO) cells	465:497	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	0	43	theme	Oligosaccharide	20:34	arg1	Structures					36:45	the Oligosaccharide Structures	16:45	the Oligosaccharide Structures of Antithrombin	16:61	A Comparison of the Oligosaccharide Structures of Antithrombin Derived from Plasma and Recombinant Using POTELLIGENT® Technology.					
31423004	8	44	theme	structural	1271:1280	arg1	differences					1282:1292	some structural differences	1266:1292	some structural differences	1266:1292	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	8	45	theme	significant	1376:1386	arg1	effect					1388:1393	no significant effect	1373:1393	no significant effect	1373:1393	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	3	46	theme	hamster	473:479	arg1	cells					493:497	Chinese hamster ovary (CHO) cells	465:497	Chinese hamster ovary (CHO) cells	465:497	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	0	47	theme	Antithrombin	50:61	arg1	Structures					36:45	the Oligosaccharide Structures	16:45	the Oligosaccharide Structures of Antithrombin	16:61	A Comparison of the Oligosaccharide Structures of Antithrombin Derived from Plasma and Recombinant Using POTELLIGENT® Technology.					
31423004	6	48	theme	glycan	950:955	arg1	different					969:977	different	969:977	different	969:977	In addition, the site-specific glycan profile was different between two ATs.					
31423004	6	48	theme	glycan	950:955	arg1	profile					957:963	the site-specific glycan profile	932:963	the site-specific glycan profile	932:963	In addition, the site-specific glycan profile was different between two ATs.					
31423004	5	49	contain	has	729:731	arg2	6-phosphate					835:845	mannose 6-phosphate	827:845	mannose 6-phosphate	827:845	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	49	contain	has	729:731	arg1	rhAT					724:727	The rhAT	720:727	The rhAT	720:727	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	49	contain	has	729:731	arg2	structures					754:763	more variable glycan structures	733:763	more variable glycan structures	733:763	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	49	contain	has	729:731	arg2	type					798:801	tetra-antennary complex type	774:801	tetra-antennary complex type	774:801	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	49	contain	has	729:731	arg2	type					817:820	high-mannose type	804:820	high-mannose type	804:820	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	5	49	contain	has	729:731	arg2	components					864:873	minor components	858:873	minor components compared to plasma-derived human AT (phAT)	858:916	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	8	50	dep	rhAT	1317:1320	arg1	the					1313:1315	the	1313:1315	the	1313:1315	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	2	51	theme	oligosaccharide	339:353	arg1	structure					355:363	The main oligosaccharide structure	330:363	The main oligosaccharide structure of human AT	330:375	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	6	52	theme	site-specific	936:948	arg1	different					969:977	different	969:977	different	969:977	In addition, the site-specific glycan profile was different between two ATs.					
31423004	6	52	theme	site-specific	936:948	arg1	profile					957:963	the site-specific glycan profile	932:963	the site-specific glycan profile	932:963	In addition, the site-specific glycan profile was different between two ATs.					
31423004	7	53	theme	site-specific	1184:1196	arg1	profiles					1214:1221	site-specific oligosaccharide profiles	1184:1221	site-specific oligosaccharide profiles	1184:1221	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	54	theme	profiles	1214:1221	arg1	terms					1130:1134	terms	1130:1134	terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1130:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	1	55	theme	Human	130:134	arg1	antithrombin					136:147	Human antithrombin	130:147	Human antithrombin (AT)	130:152	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	1	55	theme	Human	130:134	arg1	AT					150:151	AT	150:151	AT	150:151	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	8	56	theme	rhAT	1425:1428	arg1	safety					1415:1420	safety	1415:1420	safety	1415:1420	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	8	56	theme	rhAT	1425:1428	arg1	efficacy					1402:1409	efficacy	1402:1409	efficacy	1402:1409	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	7	57	theme	properties	1029:1038	arg1	effect					1013:1018	the effect	1009:1018	the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1009:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	3	58	theme	CHO	488:490	arg1	cells					493:497	Chinese hamster ovary (CHO) cells	465:497	Chinese hamster ovary (CHO) cells	465:497	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	8	59	dep	efficacy	1402:1409	arg1	the					1398:1400	the	1398:1400	the	1398:1400	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	7	60	theme	β-forms	1248:1254	arg1	profiles					1214:1221	site-specific oligosaccharide profiles	1184:1221	site-specific oligosaccharide profiles	1184:1221	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	60	theme	β-forms	1248:1254	arg1	ratio					1232:1236	the ratio	1228:1236	the ratio of α- and β-forms	1228:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	60	theme	β-forms	1248:1254	arg1	structures					1172:1181	their respective oligosaccharide structures	1139:1181	their respective oligosaccharide structures	1139:1181	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	8	61	contain	have	1368:1371	arg1	differences					1356:1366	these differences	1350:1366	these differences	1350:1366	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	8	61	contain	have	1368:1371	arg2	effect					1388:1393	no significant effect	1373:1393	no significant effect	1373:1393	Although some structural differences were found between the rhAT and phAT, we concluded that these differences have no significant effect on the efficacy and safety of rhAT.					
31423004	4	62	from	cells	699:703	arg1	production					708:717	production	708:717	production	708:717	However, rhAT lacking core-fucose oligosaccharides can be produced by POTELLIGENT® technology, which uses FUT8 knockout CHO cells in production.					
31423004	1	63	theme	predominant	184:194	arg1	α-form					196:201	the predominant α-form	180:201	the predominant α-form	180:201	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
31423004	3	64	gly	core-fucosylated	540:555	arg1	oligosaccharides					557:572	core-fucosylated oligosaccharides	540:572	core-fucosylated oligosaccharides	540:572	Generally, Chinese hamster ovary (CHO) cells produce recombinant human AT (rhAT) with core-fucosylated oligosaccharides.					
31423004	7	65	theme	ratio	1232:1236	arg1	terms					1130:1134	terms	1130:1134	terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1130:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	5	66	theme	high-mannose	804:815	arg1	type					817:820	high-mannose type	804:820	high-mannose type	804:820	The rhAT has more variable glycan structures, such as tetra-antennary complex type, high-mannose type, and mannose 6-phosphate species as minor components compared to plasma-derived human AT (phAT).					
31423004	7	67	from	phAT	1122:1125	arg1	terms					1130:1134	terms	1130:1134	terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms	1130:1254	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	7	68	theme	respective	1145:1154	arg1	structures					1172:1181	their respective oligosaccharide structures	1139:1181	their respective oligosaccharide structures	1139:1181	We evaluated the effect of these properties on efficacy and safety based on a comparison of rhAT made by that technology with phAT in terms of their respective oligosaccharide structures, site-specific oligosaccharide profiles, and the ratio of α- and β-forms.					
31423004	2	69	theme	human	368:372	arg1	AT					374:375	human AT	368:375	human AT	368:375	The main oligosaccharide structure of human AT consists of biantennary complex-type oligosaccharides lacking a core fucose.					
31423004	1	70	theme	possible	231:238	arg1	sites					254:258	all four possible glycosylation sites	222:258	all four possible glycosylation sites	222:258	Human antithrombin (AT) has two isoforms of which the predominant α-form is glycosylated on all four possible glycosylation sites and the lower abundant β-isoform lacks the oligosaccharide on Asn135.					
29593217	0	0	theme	natively-glycosylated	96:116	arg1	envelope					124:131	natively-glycosylated HIV-1 envelope	96:131	natively-glycosylated HIV-1 envelope	96:131	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	5	1	theme	V3-loop	993:999	arg1	base					1001:1004	the V3-loop base	989:1004	the V3-loop base	989:1004	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	6	2	theme	V3/N332gp120	1171:1182	arg1	bNAbs					1184:1188	V3/N332gp120 bNAbs	1171:1188	V3/N332gp120 bNAbs therapeutically	1171:1204	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	4	3	theme	gp120	792:796	arg1	motif					811:815	the gp120 GDIR peptide motif	788:815	the gp120 GDIR peptide motif	788:815	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	1	4	theme	HIV-1-infected	188:201	arg1	individuals					203:213	HIV-1-infected individuals	188:213	HIV-1-infected individuals	188:213	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	5	5	theme	N386gp120	966:974	arg1	glycans					976:982	N392gp120 and N386gp120 glycans	952:982	N392gp120 and N386gp120 glycans near the V3-loop base	952:1004	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	2	6	theme	protein	418:424	arg1	epitopes					426:433	most protein epitopes	413:433	most protein epitopes on HIV-1 envelope (Env)	413:457	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	3	7	theme	glycosylated	561:572	arg1	trimers					578:584	natively glycosylated Env trimers	552:584	natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date	552:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	2	8	theme	native	349:354	arg1	oligomannose					356:367	native oligomannose	349:367	native oligomannose	349:367	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	4	9	theme	peptide	803:809	arg1	motif					811:815	the gp120 GDIR peptide motif	788:815	the gp120 GDIR peptide motif	788:815	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	6	10	theme	Env	1084:1086	arg1	structures					1088:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	0	11	theme	HIV-1	118:122	arg1	envelope					124:131	natively-glycosylated HIV-1 envelope	96:131	natively-glycosylated HIV-1 envelope	96:131	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	1	12	attach	isolated	174:181	arg1	individuals					203:213	HIV-1-infected individuals	188:213	HIV-1-infected individuals	188:213	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	1	12	attach	isolated	174:181	arg2	bNAbs					167:171	bNAbs	167:171	bNAbs	167:171	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	1	12	attach	isolated	174:181	arg2	antibodies					155:164	Broadly neutralizing antibodies	134:164	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals	134:213	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	5	13	theme	V1-loop	1044:1050	arg1	components					1026:1035	protein components	1018:1035	protein components of the V1-loop	1018:1050	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	3	14	gly	glycosylated	561:572	arg1	trimers					578:584	natively glycosylated Env trimers	552:584	natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date	552:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	5	15	theme	additional	927:936	arg1	contacts					938:945	additional contacts	927:945	additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base	927:1004	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	2	16	theme	most	413:416	arg1	epitopes					426:433	most protein epitopes	413:433	most protein epitopes on HIV-1 envelope (Env)	413:457	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	5	17	theme	protein	1018:1024	arg1	components					1026:1035	protein components	1018:1035	protein components of the V1-loop	1018:1050	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	5	18	theme	binding	898:904	arg1	orientation					906:916	BG18's binding orientation	891:916	BG18's binding orientation	891:916	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	3	19	theme	trimers	578:584	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	20	theme	crystal	476:482	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	20	theme	crystal	476:482	arg1	structures					484:493	crystal structures	476:493	crystal structures	476:493	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	2	21	dep	understanding	302:314	arg1	interact					331:338	interact	331:338	interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env)	331:457	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	6	22	theme	BG18-natively-glycosylated	1057:1082	arg1	structures					1088:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	4	23	with	residues	749:756	arg1	glycan					777:782	the N332gp120 glycan	763:782	the N332gp120 glycan	763:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	2	24	from	epitopes	426:433	arg1	Env					454:456	Env	454:456	Env	454:456	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	2	24	from	epitopes	426:433	arg1	envelope					444:451	HIV-1 envelope	438:451	HIV-1 envelope (Env)	438:457	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	0	25	gly	natively-glycosylated	96:116	arg1	envelope					124:131	natively-glycosylated HIV-1 envelope	96:131	natively-glycosylated HIV-1 envelope	96:131	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	4	26	theme	GDIR	798:801	arg1	motif					811:815	the gp120 GDIR peptide motif	788:815	the gp120 GDIR peptide motif	788:815	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	1	27	theme	HIV-1	222:226	arg1	efforts					243:249	HIV-1 vaccine design efforts	222:249	HIV-1 vaccine design efforts	222:249	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	0	28	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody	0:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	2	29	theme	complex-type	373:384	arg1	N-glycan					386:393	complex-type N-glycan shield	373:400	complex-type N-glycan shield	373:400	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	1	30	theme	vaccine	228:234	arg1	efforts					243:249	HIV-1 vaccine design efforts	222:249	HIV-1 vaccine design efforts	222:249	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	2	31	with	bNAbs	263:267	arg1	efficacy					284:291	increased efficacy	274:291	increased efficacy	274:291	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	1	32	theme	design	236:241	arg1	efforts					243:249	HIV-1 vaccine design efforts	222:249	HIV-1 vaccine design efforts	222:249	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	3	33	theme	potent	616:621	arg1	bNAb					653:656	the most potent V3/N332gp120 glycan-targeting bNAb	607:656	the most potent V3/N332gp120 glycan-targeting bNAb reported to date	607:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	33	theme	potent	616:621	arg1	BG18					601:604	BG18	601:604	BG18	601:604	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	4	34	theme	distinct	824:831	arg1	orientation					845:855	a distinct Env-binding orientation	822:855	a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs	822:888	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	1	35	theme	Broadly	134:140	arg1	bNAbs					167:171	bNAbs	167:171	bNAbs	167:171	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	1	35	theme	Broadly	134:140	arg1	antibodies					155:164	Broadly neutralizing antibodies	134:164	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals	134:213	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	3	36	theme	free	520:523	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	0	37	theme	V3-glycan	47:55	arg1	antibody					78:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	4	38	theme	D	734:734	arg1	residues					749:756	common D gene-encoded residues	727:756	common D gene-encoded residues with the N332gp120 glycan	727:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	4	39	theme	common	727:732	arg1	residues					749:756	common D gene-encoded residues	727:756	common D gene-encoded residues with the N332gp120 glycan	727:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	0	40	theme	highly-potent	33:45	arg1	antibody					78:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	2	41	theme	increased	274:282	arg1	efficacy					284:291	increased efficacy	274:291	increased efficacy	274:291	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	3	42	theme	3.8-Å	508:512	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	0	43	theme	neutralizing	65:76	arg1	antibody					78:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	6	44	theme	critical	1152:1159	arg1	interactions					1139:1150	bNAb-glycan interactions	1127:1150	bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design	1127:1253	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	3	45	theme	V3/N332gp120	623:634	arg1	bNAb					653:656	the most potent V3/N332gp120 glycan-targeting bNAb	607:656	the most potent V3/N332gp120 glycan-targeting bNAb reported to date	607:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	45	theme	V3/N332gp120	623:634	arg1	BG18					601:604	BG18	601:604	BG18	601:604	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	1	46	theme	neutralizing	142:153	arg1	bNAbs					167:171	bNAbs	167:171	bNAbs	167:171	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	1	46	theme	neutralizing	142:153	arg1	antibodies					155:164	Broadly neutralizing antibodies	134:164	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals	134:213	Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts.					
29593217	0	47	theme	broadly	57:63	arg1	antibody					78:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	a highly-potent V3-glycan broadly neutralizing antibody	31:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	3	48	theme	glycan-targeting	636:651	arg1	bNAb					653:656	the most potent V3/N332gp120 glycan-targeting bNAb	607:656	the most potent V3/N332gp120 glycan-targeting bNAb reported to date	607:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	48	theme	glycan-targeting	636:651	arg1	BG18					601:604	BG18	601:604	BG18	601:604	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	6	49	gly	BG18-natively-glycosylated	1057:1082	arg1	structures					1088:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures	1053:1097	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	2	50	dep	oligomannose	356:367	arg1	the					345:347	the	345:347	the	345:347	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	6	51	theme	bNAb-glycan	1127:1137	arg1	interactions					1139:1150	bNAb-glycan interactions	1127:1150	bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design	1127:1253	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	4	52	theme	PGT121/10-1074	869:882	arg1	bNAbs					884:888	PGT121/10-1074 bNAbs	869:888	PGT121/10-1074 bNAbs	869:888	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	2	53	dep	N-glycan	386:393	arg1	shield					395:400	shield	395:400	shield	395:400	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	5	54	theme	N392gp120	952:960	arg1	glycans					976:982	N392gp120 and N386gp120 glycans	952:982	N392gp120 and N386gp120 glycans near the V3-loop base	952:1004	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	6	55	theme	interactions	1139:1150	arg1	understanding					1110:1122	understanding	1110:1122	understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design	1110:1253	The BG18-natively-glycosylated Env structures facilitate understanding of bNAb-glycan interactions critical for using V3/N332gp120 bNAbs therapeutically and targeting their epitope for immunogen design.					
29593217	5	56	with	contacts	938:945	arg1	glycans					976:982	N392gp120 and N386gp120 glycans	952:982	N392gp120 and N386gp120 glycans near the V3-loop base	952:1004	BG18's binding orientation provides additional contacts with N392gp120 and N386gp120 glycans near the V3-loop base and engages protein components of the V1-loop.					
29593217	4	57	theme	Env-binding	833:843	arg1	orientation					845:855	a distinct Env-binding orientation	822:855	a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs	822:888	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	3	58	theme	laser	534:538	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	3	59	theme	electron	525:532	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	4	60	theme	gene-encoded	736:747	arg1	residues					749:756	common D gene-encoded residues	727:756	common D gene-encoded residues with the N332gp120 glycan	727:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	4	61	theme	conserved	696:704	arg1	contacts					706:713	conserved contacts	696:713	conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan	696:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	3	62	theme	X-ray	514:518	arg1	dataset					540:546	a 3.8-Å X-ray free electron laser dataset	506:546	a 3.8-Å X-ray free electron laser dataset	506:546	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	2	63	theme	HIV-1	438:442	arg1	Env					454:456	Env	454:456	Env	454:456	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	2	63	theme	HIV-1	438:442	arg1	envelope					444:451	HIV-1 envelope	438:451	HIV-1 envelope (Env)	438:457	Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env).					
29593217	0	64	theme	antibody	78:85	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody	0:85	Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope.					
29593217	4	65	theme	relative	857:864	arg1	orientation					845:855	a distinct Env-binding orientation	822:855	a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs	822:888	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
29593217	3	66	theme	Env	574:576	arg1	trimers					578:584	natively glycosylated Env trimers	552:584	natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date	552:673	Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date.					
29593217	4	67	theme	N332gp120	767:775	arg1	glycan					777:782	the N332gp120 glycan	763:782	the N332gp120 glycan	763:782	Our structures show conserved contacts mediated by common D gene-encoded residues with the N332gp120 glycan and the gp120 GDIR peptide motif, but a distinct Env-binding orientation relative to PGT121/10-1074 bNAbs.					
31521285	0	0	theme	citrus	85:90	arg1	Hallabong					92:100	Korean citrus Hallabong	78:100	Korean citrus Hallabong	78:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	5	1	theme	1→4	735:737	arg1	unit					768:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	0	2	theme	Korean	78:83	arg1	Hallabong					92:100	Korean citrus Hallabong	78:100	Korean citrus Hallabong	78:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	0	3	from	elucidation	11:21	arg1	peels					69:73	the peels	65:73	the peels of Korean citrus Hallabong	65:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	6	4	theme	anti-metastatic	1155:1169	arg1	activity					1171:1178	the anti-metastatic activity	1151:1178	the anti-metastatic activity of HBE-III	1151:1189	The results of our 11B-NMR analysis suggest that HBE-III exists as an RG-II dimer and this structure contributes to the anti-metastatic activity of HBE-III.					
31521285	5	5	theme	chain	975:979	arg1	D					981:981	side chain D	970:981	side chain D	970:981	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	5	theme	chain	975:979	arg1	-Dha					964:967	Araf-(1→5)-Dha	954:967	Araf-(1→5)-Dha (side chain D)	954:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	3	6	theme	10 kDa	334:339	arg1	polysaccharide					348:361	a 10 kDa acidic polysaccharide	332:361	a 10 kDa acidic polysaccharide	332:361	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	6	theme	10 kDa	334:339	arg1	HBE-III					321:327	HBE-III	321:327	HBE-III	321:327	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	5	7	theme	rhamnogalacturonan	1012:1029	arg1	characteristic					994:1007	characteristic	994:1007	characteristic	994:1007	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	7	theme	rhamnogalacturonan	1012:1029	arg1	chain					719:723	a main chain	712:723	a main chain with a α-(1→4)-galacturono-oligosaccharide unit	712:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	8	theme	Rhap-	919:923	arg1	-Kdo					929:932	Rhap-(1→5)-Kdo	919:932	Rhap-(1→5)-Kdo (side chain C)	919:947	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	8	theme	Rhap-	919:923	arg1	C					946:946	side chain C	935:946	side chain C	935:946	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	0	9	theme	Hallabong	92:100	arg1	peels					69:73	the peels	65:73	the peels of Korean citrus Hallabong	65:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	6	10	theme	11B-NMR	1054:1060	arg1	analysis					1062:1069	our 11B-NMR analysis	1050:1069	our 11B-NMR analysis	1050:1069	The results of our 11B-NMR analysis suggest that HBE-III exists as an RG-II dimer and this structure contributes to the anti-metastatic activity of HBE-III.					
31521285	4	11	theme	resulting	577:585	arg1	fragments					587:595	the resulting fragments	573:595	the resulting fragments	573:595	To determine its microstructure, sequential acid hydrolysis was carried out and the resulting fragments were analyzed using composition, methylation, and MS/MS analyses.					
31521285	5	12	theme	acid-containing	872:886	arg1	nonasaccharide					888:901	aceric acid-containing nonasaccharide	865:901	aceric acid-containing nonasaccharide (side chain B)	865:916	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	12	theme	acid-containing	872:886	arg1	B					915:915	side chain B	904:915	side chain B	904:915	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	13	theme	1→5	925:927	arg1	-Kdo					929:932	Rhap-(1→5)-Kdo	919:932	Rhap-(1→5)-Kdo (side chain C)	919:947	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	13	theme	1→5	925:927	arg1	C					946:946	side chain C	935:946	side chain C	935:946	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	14	theme	aceric	865:870	arg1	nonasaccharide					888:901	aceric acid-containing nonasaccharide	865:901	aceric acid-containing nonasaccharide (side chain B)	865:916	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	14	theme	aceric	865:870	arg1	B					915:915	side chain B	904:915	side chain B	904:915	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	15	theme	-galacturono-oligosaccharide	739:766	arg1	unit					768:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	16	theme	chain	909:913	arg1	nonasaccharide					888:901	aceric acid-containing nonasaccharide	865:901	aceric acid-containing nonasaccharide (side chain B)	865:916	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	16	theme	chain	909:913	arg1	B					915:915	side chain B	904:915	side chain B	904:915	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	4	17	theme	MS/MS	647:651	arg1	analyses					653:660	MS/MS analyses	647:660	MS/MS analyses	647:660	To determine its microstructure, sequential acid hydrolysis was carried out and the resulting fragments were analyzed using composition, methylation, and MS/MS analyses.					
31521285	2	18	theme	HBE-III	297:303	arg1	structure					284:292	the primary structure	272:292	the primary structure of HBE-III	272:303	In this paper, the primary structure of HBE-III was elucidated.					
31521285	5	19	theme	side	850:853	arg1	A					861:861	side chain A	850:861	side chain A	850:861	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	19	theme	side	850:853	arg1	octasaccharide					834:847	an uronic acid-rich octasaccharide	814:847	an uronic acid-rich octasaccharide (side chain A)	814:862	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	0	20	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.	0:101	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	3	21	contain	contains	370:377	arg1	polysaccharide					348:361	a 10 kDa acidic polysaccharide	332:361	a 10 kDa acidic polysaccharide	332:361	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	21	contain	contains	370:377	arg2	monosaccharides					392:406	15 different monosaccharides	379:406	15 different monosaccharides	379:406	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	21	contain	contains	370:377	arg1	HBE-III					321:327	HBE-III	321:327	HBE-III	321:327	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	21	contain	contains	370:377	arg2	linkages					424:431	23 glycosyl linkages	412:431	23 glycosyl linkages	412:431	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	22	theme	glycosyl	415:422	arg1	linkages					424:431	23 glycosyl linkages	412:431	23 glycosyl linkages	412:431	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	5	23	theme	side	792:795	arg1	chains					797:802	side chains	792:802	side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D)	792:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	24	theme	side	904:907	arg1	nonasaccharide					888:901	aceric acid-containing nonasaccharide	865:901	aceric acid-containing nonasaccharide (side chain B)	865:916	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	24	theme	side	904:907	arg1	B					915:915	side chain B	904:915	side chain B	904:915	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	25	with	groups	782:787	arg1	unit					768:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	26	theme	chains	797:802	arg1	characteristic					994:1007	characteristic	994:1007	characteristic	994:1007	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	26	theme	chains	797:802	arg1	chain					719:723	a main chain	712:723	a main chain with a α-(1→4)-galacturono-oligosaccharide unit	712:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	26	theme	chains	797:802	arg1	groups					782:787	four groups	777:787	four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D)	777:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	26	theme	chains	797:802	arg1	chains					797:802	side chains	792:802	side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D)	792:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	2	27	theme	primary	276:282	arg1	structure					284:292	the primary structure	272:292	the primary structure of HBE-III	272:303	In this paper, the primary structure of HBE-III was elucidated.					
31521285	0	28	theme	polysaccharide	45:58	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.	0:101	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	5	29	theme	chain	855:859	arg1	A					861:861	side chain A	850:861	side chain A	850:861	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	29	theme	chain	855:859	arg1	octasaccharide					834:847	an uronic acid-rich octasaccharide	814:847	an uronic acid-rich octasaccharide (side chain A)	814:862	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	30	theme	uronic	817:822	arg1	A					861:861	side chain A	850:861	side chain A	850:861	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	30	theme	uronic	817:822	arg1	octasaccharide					834:847	an uronic acid-rich octasaccharide	814:847	an uronic acid-rich octasaccharide (side chain A)	814:862	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	0	31	theme	anti-metastatic	29:43	arg1	polysaccharide					45:58	an anti-metastatic polysaccharide	26:58	an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong	26:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	5	32	theme	side	970:973	arg1	D					981:981	side chain D	970:981	side chain D	970:981	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	32	theme	side	970:973	arg1	-Dha					964:967	Araf-(1→5)-Dha	954:967	Araf-(1→5)-Dha (side chain D)	954:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	6	33	theme	RG-II	1105:1109	arg1	dimer					1111:1115	an RG-II dimer	1102:1115	an RG-II dimer	1102:1115	The results of our 11B-NMR analysis suggest that HBE-III exists as an RG-II dimer and this structure contributes to the anti-metastatic activity of HBE-III.					
31521285	1	34	theme	Korean	196:201	arg1	Hallabong					210:218	the Korean citrus Hallabong	192:218	the Korean citrus Hallabong	192:218	Previously, we have reported that the polysaccharide, HBE-III, purified from the peel of the Korean citrus Hallabong, potently inhibits tumor-metastasis.					
31521285	5	35	theme	main	714:717	arg1	characteristic					994:1007	characteristic	994:1007	characteristic	994:1007	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	35	theme	main	714:717	arg1	chain					719:723	a main chain	712:723	a main chain with a α-(1→4)-galacturono-oligosaccharide unit	712:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	0	36	from	peels	69:73	arg1	polysaccharide					45:58	an anti-metastatic polysaccharide	26:58	an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong	26:100	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	0	36	from	peels	69:73	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.	0:101	Structural elucidation of an anti-metastatic polysaccharide from the peels of Korean citrus Hallabong.					
31521285	3	37	theme	complex	469:475	arg1	it					445:446	it	445:446	it	445:446	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	37	theme	complex	469:475	arg1	polysaccharide					477:490	a highly branched complex polysaccharide	451:490	a highly branched complex polysaccharide	451:490	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	38	theme	different	382:390	arg1	monosaccharides					392:406	15 different monosaccharides	379:406	15 different monosaccharides	379:406	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	4	39	theme	acid	537:540	arg1	hydrolysis					542:551	sequential acid hydrolysis	526:551	sequential acid hydrolysis	526:551	To determine its microstructure, sequential acid hydrolysis was carried out and the resulting fragments were analyzed using composition, methylation, and MS/MS analyses.					
31521285	5	40	theme	Araf-	954:958	arg1	D					981:981	side chain D	970:981	side chain D	970:981	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	40	theme	Araf-	954:958	arg1	-Dha					964:967	Araf-(1→5)-Dha	954:967	Araf-(1→5)-Dha (side chain D)	954:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	41	with	chain	719:723	arg1	unit					768:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	4	42	theme	sequential	526:535	arg1	hydrolysis					542:551	sequential acid hydrolysis	526:551	sequential acid hydrolysis	526:551	To determine its microstructure, sequential acid hydrolysis was carried out and the resulting fragments were analyzed using composition, methylation, and MS/MS analyses.					
31521285	6	43	theme	HBE-III	1183:1189	arg1	activity					1171:1178	the anti-metastatic activity	1151:1178	the anti-metastatic activity of HBE-III	1151:1189	The results of our 11B-NMR analysis suggest that HBE-III exists as an RG-II dimer and this structure contributes to the anti-metastatic activity of HBE-III.					
31521285	1	44	theme	citrus	203:208	arg1	Hallabong					210:218	the Korean citrus Hallabong	192:218	the Korean citrus Hallabong	192:218	Previously, we have reported that the polysaccharide, HBE-III, purified from the peel of the Korean citrus Hallabong, potently inhibits tumor-metastasis.					
31521285	5	45	theme	1→5	960:962	arg1	D					981:981	side chain D	970:981	side chain D	970:981	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	45	theme	1→5	960:962	arg1	-Dha					964:967	Araf-(1→5)-Dha	954:967	Araf-(1→5)-Dha (side chain D)	954:982	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	6	46	theme	analysis	1062:1069	arg1	results					1039:1045	The results	1035:1045	The results of our 11B-NMR analysis	1035:1069	The results of our 11B-NMR analysis suggest that HBE-III exists as an RG-II dimer and this structure contributes to the anti-metastatic activity of HBE-III.					
31521285	3	47	theme	branched	460:467	arg1	it					445:446	it	445:446	it	445:446	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	47	theme	branched	460:467	arg1	polysaccharide					477:490	a highly branched complex polysaccharide	451:490	a highly branched complex polysaccharide	451:490	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	1	48	theme	Hallabong	210:218	arg1	peel					184:187	the peel	180:187	the peel of the Korean citrus Hallabong	180:218	Previously, we have reported that the polysaccharide, HBE-III, purified from the peel of the Korean citrus Hallabong, potently inhibits tumor-metastasis.					
31521285	5	49	theme	α-	732:733	arg1	unit					768:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	a α-(1→4)-galacturono-oligosaccharide unit	730:771	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	50	theme	acid-rich	824:832	arg1	A					861:861	side chain A	850:861	side chain A	850:861	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	50	theme	acid-rich	824:832	arg1	octasaccharide					834:847	an uronic acid-rich octasaccharide	814:847	an uronic acid-rich octasaccharide (side chain A)	814:862	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	51	theme	side	935:938	arg1	-Kdo					929:932	Rhap-(1→5)-Kdo	919:932	Rhap-(1→5)-Kdo (side chain C)	919:947	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	51	theme	side	935:938	arg1	C					946:946	side chain C	935:946	side chain C	935:946	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	52	theme	chain	940:944	arg1	-Kdo					929:932	Rhap-(1→5)-Kdo	919:932	Rhap-(1→5)-Kdo (side chain C)	919:947	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	5	52	theme	chain	940:944	arg1	C					946:946	side chain C	935:946	side chain C	935:946	The results indicate that HBE-III is composed of a main chain with a α-(1→4)-galacturono-oligosaccharide unit and four groups of side chains including an uronic acid-rich octasaccharide (side chain A), aceric acid-containing nonasaccharide (side chain B), Rhap-(1→5)-Kdo (side chain C), and Araf-(1→5)-Dha (side chain D), which is characteristic of rhamnogalacturonan II.					
31521285	1	53	theme	polysaccharide	141:154	arg1	HBE-III					157:163	the polysaccharide, HBE-III	137:163	HBE-III	157:163	Previously, we have reported that the polysaccharide, HBE-III, purified from the peel of the Korean citrus Hallabong, potently inhibits tumor-metastasis.					
31521285	3	54	theme	acidic	341:346	arg1	polysaccharide					348:361	a 10 kDa acidic polysaccharide	332:361	a 10 kDa acidic polysaccharide	332:361	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
31521285	3	54	theme	acidic	341:346	arg1	HBE-III					321:327	HBE-III	321:327	HBE-III	321:327	HBE-III is a 10 kDa acidic polysaccharide, which contains 15 different monosaccharides and 23 glycosyl linkages, indicating it is a highly branched complex polysaccharide.					
30464342	10	0	with	infection	1425:1433	arg1	MRSA					1456:1459	tarP-expressing MRSA	1440:1459	tarP-expressing MRSA	1440:1459	Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences.					
30464342	6	1	theme	prominent	810:818	arg1	CC5					879:881	CC5	879:881	CC5	879:881	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	1	theme	prominent	810:818	arg1	clones					872:877	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	1	theme	prominent	810:818	arg1	CC398					887:891	CC398	887:891	CC398	887:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	8	2	theme	lower	1186:1190	arg1	levels					1192:1197	7.5-40-fold lower levels	1174:1197	7.5-40-fold lower levels of immunoglobulin G	1174:1217	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	12	3	theme	immune	1786:1791	arg1	evasion					1793:1799	an immune evasion	1783:1799	an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA	1783:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	11	4	theme	High-resolution	1548:1562	arg1	analyses					1575:1582	High-resolution structural analyses	1548:1582	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc)	1548:1658	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	11	5	theme	RboP	1693:1696	arg1	glycosylation					1698:1710	altered RboP glycosylation	1685:1710	altered RboP glycosylation	1685:1710	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	7	6	theme	important	1097:1105	arg1	consequences					1107:1118	important consequences	1097:1118	important consequences for immune recognition	1097:1141	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	6	7	theme	considerable	779:790	arg1	proportion					792:801	a considerable proportion	777:801	a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	777:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	8	8	theme	G	1217:1217	arg1	levels					1192:1197	7.5-40-fold lower levels	1174:1197	7.5-40-fold lower levels of immunoglobulin G	1174:1217	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	5	9	theme	evasion	647:653	arg1	due					678:680	due	678:680	due	678:680	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	5	9	theme	evasion	647:653	arg1	capacities					655:664	the immune evasion capacities	636:664	the immune evasion capacities of MRSA	636:672	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	10	10	theme	tarP-expressing	1440:1454	arg1	MRSA					1456:1459	tarP-expressing MRSA	1440:1459	tarP-expressing MRSA	1440:1459	Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences.					
30464342	4	11	theme	large	426:430	arg1	percentage					432:441	A large percentage	424:441	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer	424:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	7	12	theme	enzyme	1078:1083	arg1	TarS7					1085:1089	the standard enzyme TarS7	1065:1089	the standard enzyme TarS7	1065:1089	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	8	13	theme	TarS-modified	1232:1244	arg1	WTA					1246:1248	TarS-modified WTA	1232:1248	TarS-modified WTA	1232:1248	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	4	14	theme	aureus	474:479	arg1	WTA					508:510	WTA	508:510	WTA	508:510	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	14	theme	aureus	474:479	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	14	theme	aureus	474:479	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	7	15	theme	hydroxyl	1029:1036	arg1	RboP					1055:1058	the WTA RboP	1047:1058	the WTA RboP	1047:1058	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	7	15	theme	hydroxyl	1029:1036	arg1	group					1038:1042	a different hydroxyl group	1017:1042	a different hydroxyl group of the WTA RboP than the standard enzyme TarS7	1017:1089	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	4	16	theme	wall	488:491	arg1	WTA					508:510	WTA	508:510	WTA	508:510	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	16	theme	wall	488:491	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	16	theme	wall	488:491	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	7	17	theme	RboP	1055:1058	arg1	RboP					1055:1058	the WTA RboP	1047:1058	the WTA RboP	1047:1058	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	7	17	theme	RboP	1055:1058	arg1	group					1038:1042	a different hydroxyl group	1017:1042	a different hydroxyl group of the WTA RboP than the standard enzyme TarS7	1017:1089	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	9	18	theme	human	1273:1277	arg1	sera					1279:1282	human sera	1273:1282	human sera	1273:1282	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	6	19	theme	alternative	941:951	arg1	glycosyltransferase					957:975	an alternative WTA glycosyltransferase	938:975	an alternative WTA glycosyltransferase	938:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	19	theme	alternative	941:951	arg1	prophages					916:924	prophages	916:924	prophages that encode an alternative WTA glycosyltransferase	916:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	4	20	theme	human	446:450	arg1	antibodies					452:461	human antibodies	446:461	human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer	446:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	13	21	theme	invariant	1945:1953	arg1	antigens					1973:1980	invariant S. aureus vaccine antigens	1945:1980	invariant S. aureus vaccine antigens	1945:1980	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	1	22	theme	frequent	150:157	arg1	aureus					131:136	Staphylococcus aureus	116:136	Methicillin-resistant Staphylococcus aureus (MRSA)	94:143	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	1	22	theme	frequent	150:157	arg1	cause					159:163	a frequent cause	148:163	a frequent cause of difficult-to-treat, often fatal infections in humans1,2	148:222	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	9	23	theme	low	1299:1301	arg1	levels					1303:1308	only low levels	1294:1308	only low levels of antibodies against TarP-modified WTA	1294:1348	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	9	24	contain	contained	1284:1292	arg1	sera					1279:1282	human sera	1273:1282	human sera	1273:1282	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	9	24	contain	contained	1284:1292	arg2	levels					1303:1308	only low levels	1294:1308	only low levels of antibodies against TarP-modified WTA	1294:1348	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	4	25	theme	ribitol-phosphate	516:532	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	25	theme	ribitol-phosphate	516:532	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	1	26	from	cause	159:163	arg1	humans1,2					214:222	humans1,2	214:222	humans1,2	214:222	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	3	27	theme	vaccine	368:374	arg1	programs					388:395	Previous vaccine development programs	359:395	Previous vaccine development programs	359:395	Previous vaccine development programs have not been successful4.					
30464342	1	28	dep	difficult-to-treat	168:185	arg1	fatal					194:198	fatal	194:198	fatal	194:198	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	4	29	theme	N-acetylglucosamine	571:589	arg1	5,6					599:601	N-acetylglucosamine (GlcNAc)5,6	571:601	N-acetylglucosamine (GlcNAc)5,6	571:601	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	11	30	theme	targeted	1736:1743	arg1	inhibition					1745:1754	targeted inhibition	1736:1754	targeted inhibition of TarP	1736:1762	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	0	31	theme	cell	51:54	arg1	glycosylation					61:73	cell wall glycosylation	51:73	cell wall glycosylation	51:73	Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity.					
30464342	4	32	theme	RboP	535:538	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	32	theme	RboP	535:538	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	8	33	gly	TarP-glycosylated	1144:1160	arg1	WTA					1162:1164	TarP-glycosylated WTA	1144:1164	TarP-glycosylated WTA	1144:1164	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	13	34	theme	host	2090:2093	arg1	defences					2095:2102	human host defences	2084:2102	human host defences	2084:2102	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	6	35	theme	MRSA	867:870	arg1	CC5					879:881	CC5	879:881	CC5	879:881	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	35	theme	MRSA	867:870	arg1	clones					872:877	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	35	theme	MRSA	867:870	arg1	CC398					887:891	CC398	887:891	CC398	887:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	4	36	theme	surface	541:547	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	36	theme	surface	541:547	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	10	37	theme	host	1533:1536	arg1	defences					1538:1545	host defences	1533:1545	host defences	1533:1545	Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences.					
30464342	2	38	theme	Most	225:228	arg1	humans					230:235	Most humans	225:235	Most humans	225:235	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	2	39	contain	have	237:240	arg2	antibodies					242:251	antibodies	242:251	antibodies against S. aureus	242:269	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	2	39	contain	have	237:240	arg1	humans					230:235	Most humans	225:235	Most humans	225:235	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	12	40	theme	glycoantigen	1876:1887	arg1	WTA					1889:1891	its dominant glycoantigen WTA	1863:1891	its dominant glycoantigen WTA	1863:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	13	41	theme	new	2037:2039	arg1	strategy					2041:2048	a new strategy	2035:2048	a new strategy for rendering MRSA susceptible to human host defences	2035:2102	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	13	41	theme	new	2037:2039	arg1	development					2001:2011	the development	1997:2011	the development of TarP inhibitors	1997:2030	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	12	42	theme	aureus	1816:1821	arg1	strategy					1801:1808	an immune evasion strategy	1783:1808	an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA	1783:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	13	43	theme	inhibitors	2021:2030	arg1	strategy					2041:2048	a new strategy	2035:2048	a new strategy for rendering MRSA susceptible to human host defences	2035:2102	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	13	43	theme	inhibitors	2021:2030	arg1	development					2001:2011	the development	1997:2011	the development of TarP inhibitors	1997:2030	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	6	44	dep	clones	872:877	arg1	CC5					879:881	CC5	879:881	CC5	879:881	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	44	dep	clones	872:877	arg1	clones					872:877	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	44	dep	clones	872:877	arg1	CC398					887:891	CC398	887:891	CC398	887:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	11	45	theme	altered	1685:1691	arg1	glycosylation					1698:1710	altered RboP glycosylation	1685:1710	altered RboP glycosylation	1685:1710	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	7	46	theme	immune	1124:1129	arg1	recognition					1131:1141	immune recognition	1124:1141	immune recognition	1124:1141	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	2	47	from	patients3	348:356	arg1	protective					316:325	protective	316:325	protective	316:325	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	13	48	theme	antigens	1973:1980	arg1	identification					1927:1940	the identification	1923:1940	the identification of invariant S. aureus vaccine antigens	1923:1980	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	5	49	theme	MRSA	669:672	arg1	due					678:680	due	678:680	due	678:680	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	5	49	theme	MRSA	669:672	arg1	capacities					655:664	the immune evasion capacities	636:664	the immune evasion capacities of MRSA	636:672	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	11	50	theme	structural	1564:1573	arg1	analyses					1575:1582	High-resolution structural analyses	1548:1582	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc)	1548:1658	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	11	51	theme	glycosylation	1698:1710	arg1	mechanism					1672:1680	the mechanism	1668:1680	the mechanism of altered RboP glycosylation	1668:1710	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	5	52	theme	immune	640:645	arg1	due					678:680	due	678:680	due	678:680	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	5	52	theme	immune	640:645	arg1	capacities					655:664	the immune evasion capacities	636:664	the immune evasion capacities of MRSA	636:672	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	11	53	theme	WTA	1601:1603	arg1	components					1605:1614	WTA components	1601:1614	WTA components	1601:1614	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	1	54	theme	infections	200:209	arg1	aureus					131:136	Staphylococcus aureus	116:136	Methicillin-resistant Staphylococcus aureus (MRSA)	94:143	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	1	54	theme	infections	200:209	arg1	cause					159:163	a frequent cause	148:163	a frequent cause of difficult-to-treat, often fatal infections in humans1,2	148:222	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	8	55	theme	immunoglobulin	1202:1215	arg1	G					1217:1217	immunoglobulin G	1202:1217	immunoglobulin G	1202:1217	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	11	56	theme	TarP	1587:1590	arg1	analyses					1575:1582	High-resolution structural analyses	1548:1582	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc)	1548:1658	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	7	57	theme	standard	1069:1076	arg1	TarS7					1085:1089	the standard enzyme TarS7	1065:1089	the standard enzyme TarS7	1065:1089	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	4	58	theme	target	481:486	arg1	WTA					508:510	WTA	508:510	WTA	508:510	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	58	theme	target	481:486	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	58	theme	target	481:486	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	5	59	theme	epitopes	715:722	arg1	variation					685:693	variation	685:693	variation of dominant surface epitopes such as those associated with WTA	685:756	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	4	60	theme	teichoic	493:500	arg1	WTA					508:510	WTA	508:510	WTA	508:510	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	60	theme	teichoic	493:500	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	60	theme	teichoic	493:500	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	6	61	contain	contain	908:914	arg2	prophages					916:924	prophages	916:924	prophages that encode an alternative WTA glycosyltransferase	916:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	61	contain	contain	908:914	arg1	proportion					792:801	a considerable proportion	777:801	a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	777:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	61	contain	contain	908:914	arg2	glycosyltransferase					957:975	an alternative WTA glycosyltransferase	938:975	an alternative WTA glycosyltransferase	938:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	7	62	theme	WTA	1051:1053	arg1	RboP					1055:1058	the WTA RboP	1047:1058	the WTA RboP	1047:1058	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	6	63	theme	WTA	953:955	arg1	glycosyltransferase					957:975	an alternative WTA glycosyltransferase	938:975	an alternative WTA glycosyltransferase	938:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	63	theme	WTA	953:955	arg1	prophages					916:924	prophages	916:924	prophages that encode an alternative WTA glycosyltransferase	916:975	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	5	64	theme	dominant	698:705	arg1	epitopes					715:722	dominant surface epitopes	698:722	dominant surface epitopes such as those associated with WTA	698:756	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	5	64	theme	dominant	698:705	arg1	those					732:736	those	732:736	those	732:736	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	4	65	theme	antibodies	452:461	arg1	percentage					432:441	A large percentage	424:441	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer	424:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	7	66	theme	different	1019:1027	arg1	RboP					1055:1058	the WTA RboP	1047:1058	the WTA RboP	1047:1058	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	7	66	theme	different	1019:1027	arg1	group					1038:1042	a different hydroxyl group	1017:1042	a different hydroxyl group of the WTA RboP than the standard enzyme TarS7	1017:1089	This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS7, with important consequences for immune recognition.					
30464342	4	67	theme	S.	471:472	arg1	WTA					508:510	WTA	508:510	WTA	508:510	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	67	theme	S.	471:472	arg1	acid					502:505	S. aureus target wall teichoic acid	471:505	S. aureus target wall teichoic acid (WTA)	471:511	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	4	67	theme	S.	471:472	arg1	polymer					549:555	a ribitol-phosphate (RboP) surface polymer	514:555	a ribitol-phosphate (RboP) surface polymer	514:555	A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)5,6.					
30464342	13	68	theme	S.	1955:1956	arg1	antigens					1973:1980	invariant S. aureus vaccine antigens	1945:1980	invariant S. aureus vaccine antigens	1945:1980	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	3	69	theme	Previous	359:366	arg1	programs					388:395	Previous vaccine development programs	359:395	Previous vaccine development programs	359:395	Previous vaccine development programs have not been successful4.					
30464342	13	70	theme	vaccine	1965:1971	arg1	antigens					1973:1980	invariant S. aureus vaccine antigens	1945:1980	invariant S. aureus vaccine antigens	1945:1980	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	1	71	theme	difficult-to-treat	168:185	arg1	infections					200:209	difficult-to-treat, often fatal infections	168:209	difficult-to-treat, often fatal infections	168:209	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans1,2.					
30464342	5	72	theme	surface	707:713	arg1	epitopes					715:722	dominant surface epitopes	698:722	dominant surface epitopes such as those associated with WTA	698:756	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	5	72	theme	surface	707:713	arg1	those					732:736	those	732:736	those	732:736	It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA.					
30464342	9	73	theme	antibodies	1313:1322	arg1	levels					1303:1308	only low levels	1294:1308	only low levels of antibodies against TarP-modified WTA	1294:1348	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	2	74	from	protective	316:325	arg1	patients3					348:356	immunocompromised patients3	330:356	immunocompromised patients3	330:356	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	9	75	theme	TarP-modified	1332:1344	arg1	WTA					1346:1348	TarP-modified WTA	1332:1348	TarP-modified WTA	1332:1348	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	3	76	theme	development	376:386	arg1	programs					388:395	Previous vaccine development programs	359:395	Previous vaccine development programs	359:395	Previous vaccine development programs have not been successful4.					
30464342	13	77	dep	S.	1955:1956	arg1	aureus					1958:1963	aureus	1958:1963	aureus	1958:1963	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	13	78	theme	human	2084:2088	arg1	defences					2095:2102	human host defences	2084:2102	human host defences	2084:2102	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	13	79	theme	susceptible	2069:2079	arg1	MRSA					2064:2067	MRSA	2064:2067	MRSA susceptible to human host defences	2064:2102	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	10	80	theme	aureus	1517:1522	arg1	capacity					1502:1509	the capacity	1498:1509	the capacity of S. aureus to evade host defences	1498:1545	Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences.					
30464342	9	81	with	Consistent	1251:1260	arg1	this					1267:1270	this	1267:1270	this	1267:1270	Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA.					
30464342	0	82	theme	wall	56:59	arg1	glycosylation					61:73	cell wall glycosylation	51:73	cell wall glycosylation	51:73	Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity.					
30464342	11	83	theme	uridine	1620:1626	arg1	UDP-GlcNAc					1648:1657	UDP-GlcNAc	1648:1657	UDP-GlcNAc	1648:1657	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	11	83	theme	uridine	1620:1626	arg1	diphosphate GlcNAc					1628:1645	uridine diphosphate GlcNAc	1620:1645	uridine diphosphate GlcNAc (UDP-GlcNAc)	1620:1658	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	12	84	theme	dominant	1867:1874	arg1	WTA					1889:1891	its dominant glycoantigen WTA	1863:1891	its dominant glycoantigen WTA	1863:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	6	85	theme	clones	872:877	arg1	proportion					792:801	a considerable proportion	777:801	a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	777:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	12	86	theme	evasion	1793:1799	arg1	strategy					1801:1808	an immune evasion strategy	1783:1808	an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA	1783:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	11	87	theme	TarP	1759:1762	arg1	inhibition					1745:1754	targeted inhibition	1736:1754	targeted inhibition of TarP	1736:1762	High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP.					
30464342	12	88	theme	WTA	1889:1891	arg1	immunogenicity					1845:1858	the immunogenicity	1841:1858	the immunogenicity of its dominant glycoantigen WTA	1841:1891	Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA.					
30464342	6	89	theme	livestock-associated	846:865	arg1	CC5					879:881	CC5	879:881	CC5	879:881	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	89	theme	livestock-associated	846:865	arg1	clones					872:877	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	89	theme	livestock-associated	846:865	arg1	CC398					887:891	CC398	887:891	CC398	887:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	13	90	theme	TarP	2016:2019	arg1	inhibitors					2021:2030	TarP inhibitors	2016:2030	TarP inhibitors	2016:2030	These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.					
30464342	2	91	theme	immunocompromised	330:346	arg1	patients3					348:356	immunocompromised patients3	330:356	immunocompromised patients3	330:356	Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients3.					
30464342	8	92	theme	TarP-glycosylated	1144:1160	arg1	WTA					1162:1164	TarP-glycosylated WTA	1144:1164	TarP-glycosylated WTA	1144:1164	TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA.					
30464342	6	93	theme	healthcare-associated	820:840	arg1	CC5					879:881	CC5	879:881	CC5	879:881	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	93	theme	healthcare-associated	820:840	arg1	clones					872:877	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398	806:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	6	93	theme	healthcare-associated	820:840	arg1	CC398					887:891	CC398	887:891	CC398	887:891	Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase.					
30464342	10	94	theme	TarS-modified	1380:1392	arg1	WTA					1394:1396	TarS-modified WTA	1380:1396	TarS-modified WTA	1380:1396	Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences.					
30612270	2	0	theme	great	483:487	arg1	interest					489:496	great interest	483:496	great interest	483:496	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	9	1	theme	quantitative	1716:1727	arg1	analysis					1729:1736	qualitative and quantitative analysis	1700:1736	qualitative and quantitative analysis of the glycosylation of glycoproteins	1700:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	9	2	gly	glycosylation	1745:1757	arg1	glycoproteins					1762:1774	glycoproteins	1762:1774	glycoproteins	1762:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	2	3	theme	glycan	341:346	arg1	structures					348:357	glycan structures	341:357	glycan structures	341:357	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	0	4	theme	part	62:65	arg1	ESI/MS					33:38	ESI/MS	33:38	ESI/MS	33:38	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	7	5	gly	sialylated	1221:1230	arg1	compositions					1232:1243	highly sialylated compositions	1214:1243	highly sialylated compositions	1214:1243	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	0	6	theme	Ceruloplasmin	160:172	arg1	compositions					144:155	new glycan compositions	133:155	new glycan compositions of Ceruloplasmin	133:172	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	5	7	theme	peptide	896:902	arg1	length					882:887	the length	878:887	the length of the peptide	878:902	Here we show that an increase in the length of the peptide can lead to a more accurate determination and quantification of the glycans.					
30612270	7	8	theme	increased	1255:1263	arg1	abundance					1274:1282	an increased relative abundance	1252:1282	an increased relative abundance	1252:1282	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	3	9	attach	liberated	594:602	arg2	glycopeptides					568:580	glycopeptides	568:580	glycopeptides	568:580	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	3	9	attach	liberated	594:602	arg1	glycoprotein					613:624	the glycoprotein	609:624	the glycoprotein of interest	609:636	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	4	10	attach	present	809:815	arg2	proportions					797:807	the actual proportions	786:807	the actual proportions present in the intact glycoprotein	786:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	4	10	attach	present	809:815	arg1	glycoprotein					831:842	the intact glycoprotein	820:842	the intact glycoprotein	820:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	6	11	theme	varying	1106:1112	arg1	lengths					1122:1128	varying peptide lengths	1106:1128	varying peptide lengths	1106:1128	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	8	12	from	137N	1499:1502	arg1	ceruloplasmin					1472:1484	ceruloplasmin	1472:1484	ceruloplasmin at glycosite 137N in all 17 samples	1472:1520	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	9	13	theme	middle	1630:1635	arg1	down					1637:1640	a middle down	1628:1640	a middle down	1628:1640	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	9	13	theme	middle	1630:1635	arg1	favorable					1686:1694	favorable	1686:1694	favorable	1686:1694	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	2	14	from	interest	309:316	arg1	biomarkers					321:330	biomarkers	321:330	biomarkers based on glycan structures	321:357	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	4	15	theme	glycopeptides	673:685	arg1	properties					659:668	ionization properties	648:668	ionization properties of glycopeptides	648:685	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	7	16	theme	most	1202:1205	arg1	cases					1207:1211	most cases	1202:1211	most cases	1202:1211	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	2	17	dep	quantification	462:475	arg1	interest					489:496	great interest	483:496	great interest	483:496	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	3	18	theme	Mass	499:502	arg1	spectrometry					504:515	Mass spectrometry	499:515	Mass spectrometry	499:515	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	9	19	theme	glycoproteins	1762:1774	arg1	glycosylation					1745:1757	the glycosylation	1741:1757	the glycosylation of glycoproteins	1741:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	8	20	theme	triantennary	1351:1362	arg1	glycans					1364:1370	triantennary glycans	1351:1370	triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach	1351:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	9	21	theme	glycosylation	1745:1757	arg1	analysis					1729:1736	qualitative and quantitative analysis	1700:1736	qualitative and quantitative analysis of the glycosylation of glycoproteins	1700:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	6	22	theme	different	1047:1055	arg1	individuals					1057:1067	17 different individuals	1044:1067	17 different individuals	1044:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	4	23	theme	actual	790:795	arg1	proportions					797:807	the actual proportions	786:807	the actual proportions present in the intact glycoprotein	786:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	8	24	theme	three	1393:1397	arg1	factor					1383:1388	a factor	1381:1388	a factor of three	1381:1397	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	2	25	theme	recent	382:387	arg1	years					389:393	recent years	382:393	recent years	382:393	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	0	26	theme	relative	88:95	arg1	proportions					97:107	the relative proportions	84:107	the relative proportions	84:107	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	8	27	from	ceruloplasmin	1472:1484	arg1	samples					1514:1520	all 17 samples	1507:1520	all 17 samples	1507:1520	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	8	28	theme	up	1375:1376	arg1	peaks					1418:1422	MS peaks	1415:1422	MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach	1415:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	2	29	theme	glycosylation	438:450	arg1	quantification					462:475	their quantification	456:475	their quantification is of great interest	456:496	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	2	29	theme	glycosylation	438:450	arg1	changes					427:433	disease specific changes	410:433	disease specific changes of glycosylation	410:450	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	7	30	theme	sialylated	1221:1230	arg1	compositions					1232:1243	highly sialylated compositions	1214:1243	highly sialylated compositions	1214:1243	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	6	31	from	sites	1004:1008	arg1	individuals					1057:1067	17 different individuals	1044:1067	17 different individuals	1044:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	9	32	theme	qualitative	1700:1710	arg1	analysis					1729:1736	qualitative and quantitative analysis	1700:1736	qualitative and quantitative analysis of the glycosylation of glycoproteins	1700:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	5	33	theme	accurate	923:930	arg1	determination					932:944	a more accurate determination	916:944	a more accurate determination	916:944	Here we show that an increase in the length of the peptide can lead to a more accurate determination and quantification of the glycans.					
30612270	6	34	gly	glycopeptides	1089:1101	arg2	glycopeptides					1089:1101	glycopeptides	1089:1101	glycopeptides	1089:1101	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	34	gly	glycopeptides	1089:1101	arg1	lengths					1122:1128	varying peptide lengths	1106:1128	varying peptide lengths	1106:1128	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	1	35	theme	Significant	175:185	arg1	changes					187:193	Significant changes	175:193	Significant changes of glycan structures	175:214	Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present.					
30612270	4	36	gly	glycoprotein	831:842	arg1	glycoprotein					831:842	the intact glycoprotein	820:842	the intact glycoprotein	820:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	4	37	gly	glycopeptides	673:685	arg2	glycopeptides					673:685	glycopeptides	673:685	glycopeptides	673:685	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	6	38	theme	proteases	1163:1171	arg1	action					1143:1148	action	1143:1148	action of different proteases	1143:1171	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	3	39	gly	glycoprotein	613:624	arg1	glycoprotein					613:624	the glycoprotein	609:624	the glycoprotein of interest	609:636	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	6	40	from	individuals	1057:1067	arg1	sites					1004:1008	The four glycosylation sites	981:1008	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals	981:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	40	from	individuals	1057:1067	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	41	theme	different	1153:1161	arg1	proteases					1163:1171	different proteases	1153:1171	different proteases	1153:1171	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	2	42	theme	disease	410:416	arg1	changes					427:433	disease specific changes	410:433	disease specific changes of glycosylation	410:450	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	2	43	theme	specific	418:425	arg1	changes					427:433	disease specific changes	410:433	disease specific changes of glycosylation	410:450	Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest.					
30612270	5	44	theme	glycans	972:978	arg1	quantification					950:963	quantification	950:963	quantification	950:963	Here we show that an increase in the length of the peptide can lead to a more accurate determination and quantification of the glycans.					
30612270	5	44	theme	glycans	972:978	arg1	determination					932:944	a more accurate determination	916:944	a more accurate determination	916:944	Here we show that an increase in the length of the peptide can lead to a more accurate determination and quantification of the glycans.					
30612270	8	45	theme	tetraantennary	1441:1454	arg1	compositions					1456:1467	tetraantennary compositions	1441:1467	tetraantennary compositions	1441:1467	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	1	46	theme	glycan	198:203	arg1	structures					205:214	glycan structures	198:214	glycan structures	198:214	Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present.					
30612270	9	47	theme	down	1667:1670	arg1	approach					1674:1681	a top down - approach	1661:1681	a top down - approach	1661:1681	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	7	48	theme	relative	1265:1272	arg1	abundance					1274:1282	an increased relative abundance	1252:1282	an increased relative abundance	1252:1282	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	1	49	theme	structures	205:214	arg1	changes					187:193	Significant changes	175:193	Significant changes of glycan structures	175:214	Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present.					
30612270	3	50	used	used	534:537	arg2	spectrometry					504:515	Mass spectrometry	499:515	Mass spectrometry	499:515	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	8	51	theme	up	1562:1563	arg1	approach					1565:1572	a bottom up approach	1553:1572	a bottom up approach	1553:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	6	52	theme	glycosylation	990:1002	arg1	sites					1004:1008	The four glycosylation sites	981:1008	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals	981:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	52	theme	glycosylation	990:1002	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	1	53	located	observed	220:227	arg1	humans					232:237	humans	232:237	humans	232:237	Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present.					
30612270	1	53	located	observed	220:227	arg2	changes					187:193	Significant changes	175:193	Significant changes of glycan structures	175:214	Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present.					
30612270	6	54	gly	glycosylation	990:1002	arg2	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	54	gly	glycosylation	990:1002	arg2	individuals					1057:1067	17 different individuals	1044:1067	17 different individuals	1044:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	54	gly	glycosylation	990:1002	arg2	sites					1004:1008	The four glycosylation sites	981:1008	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals	981:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	54	gly	glycosylation	990:1002	arg2	four					985:988	four	985:988	four	985:988	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	54	gly	glycosylation	990:1002	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	4	55	theme	ionization	648:657	arg1	properties					659:668	ionization properties	648:668	ionization properties of glycopeptides	648:685	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	8	56	theme	bottom	1555:1560	arg1	approach					1565:1572	a bottom up approach	1553:1572	a bottom up approach	1553:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	0	57	theme	glycan	137:142	arg1	compositions					144:155	new glycan compositions	133:155	new glycan compositions of Ceruloplasmin	133:172	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	7	58	theme	peptide	1300:1306	arg1	length					1308:1313	peptide length	1300:1313	peptide length	1300:1313	In most cases, highly sialylated compositions showed an increased relative abundance with increasing peptide length.					
30612270	0	59	theme	new	133:135	arg1	compositions					144:155	new glycan compositions	133:155	new glycan compositions of Ceruloplasmin	133:172	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	8	60	gly	glycosite	1489:1497	arg2	glycosite					1489:1497	glycosite 137N	1489:1502	glycosite 137N	1489:1502	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	4	61	from	glycoprotein	831:842	arg1	present					809:815	present	809:815	present	809:815	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	3	62	gly	glycopeptides	568:580	arg2	glycopeptides					568:580	glycopeptides	568:580	glycopeptides	568:580	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	8	63	theme	MS	1415:1416	arg1	peaks					1418:1422	MS peaks	1415:1422	MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach	1415:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	4	64	theme	present	809:815	arg1	proportions					797:807	the actual proportions	786:807	the actual proportions present in the intact glycoprotein	786:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	3	65	theme	interest	629:636	arg1	glycoprotein					613:624	the glycoprotein	609:624	the glycoprotein of interest	609:636	Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest.					
30612270	4	66	theme	intact	824:829	arg1	glycoprotein					831:842	the intact glycoprotein	820:842	the intact glycoprotein	820:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	5	67	from	increase	866:873	arg1	length					882:887	the length	878:887	the length of the peptide	878:902	Here we show that an increase in the length of the peptide can lead to a more accurate determination and quantification of the glycans.					
30612270	6	68	theme	ceruloplasmin	1025:1037	arg1	sites					1004:1008	The four glycosylation sites	981:1008	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals	981:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	68	theme	ceruloplasmin	1025:1037	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	0	69	theme	compositions	144:155	arg1	discovery					120:128	discovery	120:128	discovery of new glycan compositions of Ceruloplasmin	120:172	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	8	70	theme	relative	1330:1337	arg1	increase					1339:1346	a relative increase	1328:1346	a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach	1328:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	8	71	theme	glycans	1364:1370	arg1	increase					1339:1346	a relative increase	1328:1346	a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach	1328:1572	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	6	72	theme	serum	1019:1023	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	0	73	theme	peptide	54:60	arg1	part					62:65	the peptide part	50:65	the peptide part	50:65	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	8	74	theme	glycosite	1489:1497	arg1	137N					1499:1502	glycosite 137N	1489:1502	glycosite 137N	1489:1502	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	9	75	gly	glycoproteins	1762:1774	arg1	glycoproteins					1762:1774	glycoproteins	1762:1774	glycoproteins	1762:1774	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
30612270	6	76	theme	lengths	1122:1128	arg1	glycopeptides					1089:1101	glycopeptides	1089:1101	glycopeptides	1089:1101	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	77	theme	limited	1180:1186	arg1	digestion					1188:1196	limited digestion	1180:1196	limited digestion	1180:1196	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	6	78	theme	human	1013:1017	arg1	ceruloplasmin					1025:1037	human serum ceruloplasmin	1013:1037	human serum ceruloplasmin from 17 different individuals	1013:1067	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	8	79	from	compositions	1456:1467	arg1	ceruloplasmin					1472:1484	ceruloplasmin	1472:1484	ceruloplasmin at glycosite 137N in all 17 samples	1472:1520	We observed a relative increase of triantennary glycans of up to a factor of three and, even more, MS peaks corresponding to tetraantennary compositions on ceruloplasmin at glycosite 137N in all 17 samples, which we did not detect using a bottom up approach.					
30612270	0	80	theme	Glycopeptides	16:28	arg1	Quantitation					0:11	Quantitation	0:11	Quantitation of Glycopeptides by ESI/MS - size of the peptide part	0:65	Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.					
30612270	4	81	from	present	809:815	arg1	glycoprotein					831:842	the intact glycoprotein	820:842	the intact glycoprotein	820:842	However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein.					
30612270	6	82	theme	peptide	1114:1120	arg1	lengths					1122:1128	varying peptide lengths	1106:1128	varying peptide lengths	1106:1128	The four glycosylation sites of human serum ceruloplasmin from 17 different individuals were analyzed using glycopeptides of varying peptide lengths, obtained by action of different proteases and by limited digestion.					
30612270	9	83	theme	top	1663:1665	arg1	approach					1674:1681	a top down - approach	1661:1681	a top down - approach	1661:1681	The data presented here leads to the conclusion that a middle down - or when possible a top down - approach is favorable for qualitative and quantitative analysis of the glycosylation of glycoproteins.					
29505873	4	0	theme	ascorbic	689:696	arg1	acid					698:701	35.81mg ascorbic acid	681:701	35.81mg ascorbic acid equivalents/g matter	681:722	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	1	1	from	degree	193:198	arg1	indicum					289:295	Arthrocnemum indicum leaves	276:302	Arthrocnemum indicum leaves	276:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	4	2	theme	equivalents/g	703:715	arg1	matter					717:722	35.81mg ascorbic acid equivalents/g matter	681:722	35.81mg ascorbic acid equivalents/g matter	681:722	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	5	3	theme	Colorimetric	750:761	arg1	assays					763:768	Colorimetric assays	750:768	Colorimetric assays	750:768	Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively.					
29505873	5	4	theme	total	782:786	arg1	sugar					788:792	total sugar	782:792	total sugar	782:792	Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively.					
29505873	9	5	theme	antiglycated	1451:1462	arg1	agents					1464:1469	possible antiglycated agents	1442:1469	possible antiglycated agents	1442:1469	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	9	5	theme	antiglycated	1451:1462	arg1	polysaccharide					1370:1383	extracted polysaccharide	1360:1383	extracted polysaccharide	1360:1383	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	1	6	from	rate	172:175	arg1	indicum					289:295	Arthrocnemum indicum leaves	276:302	Arthrocnemum indicum leaves	276:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	4	7	theme	acid	698:701	arg1	matter					717:722	35.81mg ascorbic acid equivalents/g matter	681:722	35.81mg ascorbic acid equivalents/g matter	681:722	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	3	8	theme	optimal	408:414	arg1	settings					416:423	The optimal settings	404:423	The optimal settings	404:423	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	6	9	theme	various	935:941	arg1	NMR					968:970	NMR	968:970	NMR	968:970	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	6	9	theme	various	935:941	arg1	analysis					983:990	UV-vis analysis	976:990	UV-vis analysis	976:990	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	6	9	theme	various	935:941	arg1	FT-IR					961:965	FT-IR	961:965	FT-IR	961:965	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	6	9	theme	various	935:941	arg1	methods					943:949	various methods	935:949	various methods including FT-IR, NMR and UV-vis analysis	935:990	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	4	10	theme	antiglycation	654:666	arg1	capacity					668:675	antiglycation capacity	654:675	antiglycation capacity	654:675	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	9	11	theme	extracted	1360:1368	arg1	source					1404:1409	source	1404:1409	source of natural antioxidants	1404:1433	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	9	11	theme	extracted	1360:1368	arg1	agents					1464:1469	possible antiglycated agents	1442:1469	possible antiglycated agents	1442:1469	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	9	11	theme	extracted	1360:1368	arg1	polysaccharide					1370:1383	extracted polysaccharide	1360:1383	extracted polysaccharide	1360:1383	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	6	12	theme	structure	901:909	arg1	study					911:915	Preliminary structure study	889:915	Preliminary structure study	889:915	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	3	13	dep	were	425:428	arg1	time					463:466	time	463:466	time of 288min	463:476	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	13	dep	were	425:428	arg1	temperature					442:452	extraction temperature	431:452	extraction temperature of 80°C	431:460	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	13	dep	were	425:428	arg1	ratio					498:502	(solvent/solid) ratio	482:502	(solvent/solid) ratio of 40mL/g	482:512	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	8	14	theme	extracted	1116:1124	arg1	polysaccharide					1154:1167	a pectic polysaccharide	1145:1167	a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1145:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	14	theme	extracted	1116:1124	arg1	polysaccharide					1126:1139	extracted polysaccharide	1116:1139	extracted polysaccharide	1116:1139	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	15	theme	%	1299:1299	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	16	theme	%	1284:1284	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	7	17	contain	had	1035:1037	arg2	weight					1060:1065	an average molecular weight	1039:1065	an average molecular weight of 2179kDa	1039:1076	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	7	17	contain	had	1035:1037	arg1	polysaccharide					1020:1033	polysaccharide	1020:1033	polysaccharide	1020:1033	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	6	18	theme	Preliminary	889:899	arg1	study					911:915	Preliminary structure study	889:915	Preliminary structure study	889:915	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	2	19	theme	independent	311:321	arg1	variables					323:331	Three independent variables	305:331	Three independent variables	305:331	Three independent variables were opted: extraction temperature, time and ratio (solvent/material).					
29505873	8	20	theme	molar	1252:1256	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	1	21	theme	esterification	178:191	arg1	degree					193:198	esterification degree	178:198	esterification degree	178:198	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	5	22	theme	acid	805:808	arg1	contents					810:817	total sugar and uronic acid contents	782:817	total sugar and uronic acid contents for polysaccharide	782:836	Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively.					
29505873	0	23	from	activities	45:54	arg1	indicum					93:99	Arthrocnemum indicum	80:99	Arthrocnemum indicum	80:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	5	24	theme	sugar	788:792	arg1	contents					810:817	total sugar and uronic acid contents	782:817	total sugar and uronic acid contents for polysaccharide	782:836	Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively.					
29505873	8	25	from	rhamnose	1216:1223	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	6	26	theme	UV-vis	976:981	arg1	analysis					983:990	UV-vis analysis	976:990	UV-vis analysis	976:990	Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis.					
29505873	8	27	from	glucose	1226:1232	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	3	28	theme	80°C	457:460	arg1	time					463:466	time	463:466	time of 288min	463:476	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	28	theme	80°C	457:460	arg1	temperature					442:452	extraction temperature	431:452	extraction temperature of 80°C	431:460	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	28	theme	80°C	457:460	arg1	ratio					498:502	(solvent/solid) ratio	482:502	(solvent/solid) ratio of 40mL/g	482:512	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	4	29	theme	esterification	558:571	arg1	degree					573:578	esterification degree	558:578	esterification degree	558:578	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	4	30	dep	%	598:598	arg1	matter					717:722	35.81mg ascorbic acid equivalents/g matter	681:722	35.81mg ascorbic acid equivalents/g matter	681:722	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	2	31	theme	extraction	345:354	arg1	temperature					356:366	extraction temperature	345:366	extraction temperature	345:366	Three independent variables were opted: extraction temperature, time and ratio (solvent/material).					
29505873	1	32	theme	total	201:205	arg1	ability					219:225	total antioxidant ability	201:225	total antioxidant ability	201:225	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	9	33	theme	antioxidants	1422:1433	arg1	source					1404:1409	source	1404:1409	source of natural antioxidants	1404:1433	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	9	33	theme	antioxidants	1422:1433	arg1	polysaccharide					1370:1383	extracted polysaccharide	1360:1383	extracted polysaccharide	1360:1383	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	8	34	theme	GC-MS	1089:1093	arg1	analyzes					1095:1102	GC-MS analyzes	1089:1102	GC-MS analyzes	1089:1102	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	1	35	theme	composite	117:125	arg1	design					127:132	Central composite design	109:132	Central composite design	109:132	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	36	theme	antioxidant	207:217	arg1	ability					219:225	total antioxidant ability	201:225	total antioxidant ability	201:225	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	37	theme	Central	109:115	arg1	design					127:132	Central composite design	109:132	Central composite design	109:132	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	8	38	theme	%	1306:1306	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	39	from	mannose	1196:1202	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	40	theme	%	1316:1316	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	41	from	galactose	1205:1213	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	3	42	theme	solvent/solid	483:495	arg1	ratio					498:502	(solvent/solid) ratio	482:502	(solvent/solid) ratio of 40mL/g	482:512	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	0	43	theme	activities	45:54	arg1	Optimization					0:11	Optimization	0:11	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum	0:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	8	44	from	xylose	1238:1243	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	0	45	from	indicum	93:99	arg1	activities					45:54	antioxidant and antiglycated activities	16:54	antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum	16:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	0	45	from	indicum	93:99	arg1	polysaccharides					59:73	polysaccharides	59:73	polysaccharides from Arthrocnemum indicum	59:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	4	46	theme	antioxidant	629:639	arg1	activity					641:648	total antioxidant activity	623:648	total antioxidant activity	623:648	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	3	47	theme	288min	471:476	arg1	time					463:466	time	463:466	time of 288min	463:476	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	47	theme	288min	471:476	arg1	temperature					442:452	extraction temperature	431:452	extraction temperature of 80°C	431:460	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	47	theme	288min	471:476	arg1	ratio					498:502	(solvent/solid) ratio	482:502	(solvent/solid) ratio of 40mL/g	482:512	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	0	48	theme	antiglycated	32:43	arg1	activities					45:54	antioxidant and antiglycated activities	16:54	antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum	16:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	4	49	theme	uronic	537:542	arg1	rate					549:552	uronic acid rate	537:552	uronic acid rate	537:552	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	1	50	from	indicum	289:295	arg1	carbohydrates					257:269	carbohydrates	257:269	carbohydrates from Arthrocnemum indicum leaves	257:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	50	from	indicum	289:295	arg1	ability					219:225	total antioxidant ability	201:225	total antioxidant ability	201:225	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	50	from	indicum	289:295	arg1	capacity					245:252	antiglycation capacity	231:252	antiglycation capacity	231:252	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	50	from	indicum	289:295	arg1	rate					172:175	uronic acid rate	160:175	uronic acid rate	160:175	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	50	from	indicum	289:295	arg1	degree					193:198	esterification degree	178:198	esterification degree	178:198	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	8	51	theme	%	1277:1277	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	1	52	theme	antiglycation	231:243	arg1	capacity					245:252	antiglycation capacity	231:252	antiglycation capacity	231:252	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	0	53	theme	polysaccharides	59:73	arg1	activities					45:54	antioxidant and antiglycated activities	16:54	antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum	16:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	7	54	theme	molecular	1050:1058	arg1	weight					1060:1065	an average molecular weight	1039:1065	an average molecular weight of 2179kDa	1039:1076	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	9	55	theme	natural	1414:1420	arg1	antioxidants					1422:1433	natural antioxidants	1414:1433	natural antioxidants	1414:1433	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	7	56	theme	2179kDa	1070:1076	arg1	weight					1060:1065	an average molecular weight	1039:1065	an average molecular weight of 2179kDa	1039:1076	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	3	57	theme	extraction	431:440	arg1	temperature					442:452	extraction temperature	431:452	extraction temperature of 80°C	431:460	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	58	theme	40mL/g	507:512	arg1	time					463:466	time	463:466	time of 288min	463:476	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	58	theme	40mL/g	507:512	arg1	temperature					442:452	extraction temperature	431:452	extraction temperature of 80°C	431:460	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	3	58	theme	40mL/g	507:512	arg1	ratio					498:502	(solvent/solid) ratio	482:502	(solvent/solid) ratio of 40mL/g	482:512	The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g.					
29505873	4	59	theme	acid	544:547	arg1	rate					549:552	uronic acid rate	537:552	uronic acid rate	537:552	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	1	60	from	capacity	245:252	arg1	indicum					289:295	Arthrocnemum indicum leaves	276:302	Arthrocnemum indicum leaves	276:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	61	from	ability	219:225	arg1	indicum					289:295	Arthrocnemum indicum leaves	276:302	Arthrocnemum indicum leaves	276:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	9	62	theme	possible	1442:1449	arg1	agents					1464:1469	possible antiglycated agents	1442:1469	possible antiglycated agents	1442:1469	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	9	62	theme	possible	1442:1449	arg1	polysaccharide					1370:1383	extracted polysaccharide	1360:1383	extracted polysaccharide	1360:1383	This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.					
29505873	7	63	theme	average	1042:1048	arg1	weight					1060:1065	an average molecular weight	1039:1065	an average molecular weight of 2179kDa	1039:1076	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	8	64	theme	%	1292:1292	arg1	percentage					1258:1267	the molar percentage	1248:1267	the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1248:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	7	65	theme	SEC	993:995	arg1	analyzes					997:1004	SEC analyzes	993:1004	SEC analyzes	993:1004	SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa.					
29505873	4	66	theme	35.81mg	681:687	arg1	acid					698:701	35.81mg ascorbic acid	681:701	35.81mg ascorbic acid equivalents/g matter	681:722	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	8	67	theme	pectic	1147:1152	arg1	polysaccharide					1154:1167	a pectic polysaccharide	1145:1167	a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively	1145:1330	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	8	67	theme	pectic	1147:1152	arg1	polysaccharide					1126:1139	extracted polysaccharide	1116:1139	extracted polysaccharide	1116:1139	Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively.					
29505873	1	68	dep	indicum	289:295	arg1	leaves					297:302	leaves	297:302	leaves	297:302	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	4	69	theme	total	623:627	arg1	activity					641:648	total antioxidant activity	623:648	total antioxidant activity	623:648	Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively.					
29505873	1	70	theme	uronic	160:165	arg1	rate					172:175	uronic acid rate	160:175	uronic acid rate	160:175	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	71	theme	carbohydrates	257:269	arg1	ability					219:225	total antioxidant ability	201:225	total antioxidant ability	201:225	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	71	theme	carbohydrates	257:269	arg1	capacity					245:252	antiglycation capacity	231:252	antiglycation capacity	231:252	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	71	theme	carbohydrates	257:269	arg1	rate					172:175	uronic acid rate	160:175	uronic acid rate	160:175	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	1	71	theme	carbohydrates	257:269	arg1	degree					193:198	esterification degree	178:198	esterification degree	178:198	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
29505873	5	72	theme	uronic	798:803	arg1	acid					805:808	uronic acid	798:808	uronic acid	798:808	Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively.					
29505873	0	73	theme	antioxidant	16:26	arg1	activities					45:54	antioxidant and antiglycated activities	16:54	antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum	16:99	Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves.					
29505873	1	74	theme	acid	167:170	arg1	rate					172:175	uronic acid rate	160:175	uronic acid rate	160:175	Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves.					
30532014	1	0	theme	N-linked	172:179	arg1	glycans					181:187	highly complex multi-antennary N-linked glycans	141:187	highly complex multi-antennary N-linked glycans	141:187	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	6	1	theme	appropriate	1056:1066	arg1	point					1068:1072	an appropriate point	1053:1072	an appropriate point in the enzymatic synthesis	1053:1099	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	6	2	from	point	1068:1072	arg1	synthesis					1091:1099	the enzymatic synthesis	1077:1099	the enzymatic synthesis	1077:1099	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	2	3	from	diversified	334:344	arg1	manner					392:397	a branch-selective manner	373:397	a branch-selective manner	373:397	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	4	4	theme	multi-antennary	731:745	arg1	N-glycans					747:755	multi-antennary N-glycans	731:755	multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans	731:888	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	3	5	from	progress	544:551	arg1	glycoscience					556:567	glycoscience	556:567	glycoscience	556:567	Only highly specialized laboratories can perform such syntheses, which greatly hampers progress in glycoscience.					
30532014	2	6	theme	advanced	299:306	arg1	intermediates					308:320	advanced intermediates	299:320	advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature	299:454	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	4	7	theme	available	628:636	arg1	glycopeptide					651:662	a readily available bi-antennary glycopeptide	618:662	a readily available bi-antennary glycopeptide	618:662	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	5	8	theme	unnatural	1026:1034	arg1	donors					1042:1047	unnatural sugar donors	1026:1047	unnatural sugar donors	1026:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	9	theme	additional	944:953	arg1	points					965:970	additional branching points	944:970	additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors	944:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	10	theme	key	893:895	arg1	feature					897:903	A key feature	891:903	A key feature of our approach	891:919	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	10	theme	key	893:895	arg1	installation					928:939	the installation	924:939	the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors	924:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	11	theme	sugar	1036:1040	arg1	donors					1042:1047	unnatural sugar donors	1026:1047	unnatural sugar donors	1026:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	4	12	theme	chemical	697:704	arg1	steps					720:724	ten or fewer chemical and enzymatic steps	684:724	ten or fewer chemical and enzymatic steps	684:724	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	5	13	theme	branching	955:963	arg1	points					965:970	additional branching points	944:970	additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors	944:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	2	14	theme	chemical	273:280	arg1	steps					282:286	as many as 100 chemical steps	258:286	as many as 100 chemical steps	258:286	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	2	15	theme	asymmetrical	407:418	arg1	structures					420:429	asymmetrical structures	407:429	asymmetrical structures commonly found in nature	407:454	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	5	16	from	MGAT4	990:994	arg1	combination					1009:1019	combination	1009:1019	combination with unnatural sugar donors	1009:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	4	17	theme	enzymatic	710:718	arg1	steps					720:724	ten or fewer chemical and enzymatic steps	684:724	ten or fewer chemical and enzymatic steps	684:724	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	5	18	with	combination	1009:1019	arg1	donors					1042:1047	unnatural sugar donors	1026:1047	unnatural sugar donors	1026:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	4	19	theme	biomimetic	589:598	arg1	approach					600:607	a biomimetic approach	587:607	a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans	587:888	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	2	20	from	manner	392:397	arg1	diversified					334:344	diversified	334:344	diversified	334:344	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	5	21	theme	approach	912:919	arg1	feature					897:903	A key feature	891:903	A key feature of our approach	891:919	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	21	theme	approach	912:919	arg1	installation					928:939	the installation	924:939	the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors	924:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	3	22	theme	Only	457:460	arg1	laboratories					481:492	Only highly specialized laboratories	457:492	Only highly specialized laboratories	457:492	Only highly specialized laboratories can perform such syntheses, which greatly hampers progress in glycoscience.					
30532014	4	23	theme	complex	848:854	arg1	N-glycans					880:888	highly complex asymmetrically branched N-glycans	841:888	highly complex asymmetrically branched N-glycans	841:888	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	1	24	link	N-linked	172:179	arg1	glycans					181:187	highly complex multi-antennary N-linked glycans	141:187	highly complex multi-antennary N-linked glycans	141:187	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	1	25	theme	Contemporary	90:101	arg1	approaches					118:127	Contemporary chemoenzymatic approaches	90:127	Contemporary chemoenzymatic approaches	90:127	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	6	26	theme	unnatural	1106:1114	arg1	monosaccharides					1116:1130	the unnatural monosaccharides	1102:1130	the unnatural monosaccharides	1102:1130	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	0	27	theme	chemoenzymatic	17:30	arg1	synthesis					32:40	the chemoenzymatic synthesis	13:40	the chemoenzymatic synthesis of complex N-glycans	13:61	Streamlining the chemoenzymatic synthesis of complex N-glycans by a stop and go strategy.					
30532014	6	28	theme	unique	1223:1228	arg1	appendage					1230:1238	a unique appendage	1221:1238	a unique appendage	1221:1238	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	1	29	theme	chemoenzymatic	103:116	arg1	approaches					118:127	Contemporary chemoenzymatic approaches	90:127	Contemporary chemoenzymatic approaches	90:127	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	3	30	theme	such	506:509	arg1	syntheses					511:519	such syntheses	506:519	such syntheses	506:519	Only highly specialized laboratories can perform such syntheses, which greatly hampers progress in glycoscience.					
30532014	0	31	theme	complex	45:51	arg1	N-glycans					53:61	complex N-glycans	45:61	complex N-glycans	45:61	Streamlining the chemoenzymatic synthesis of complex N-glycans by a stop and go strategy.					
30532014	5	32	from	MGAT5	1000:1004	arg1	combination					1009:1019	combination	1009:1019	combination with unnatural sugar donors	1009:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	4	33	dep	complex	848:854	arg1	branched					871:878	branched	871:878	branched	871:878	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	2	34	located	found	440:444	arg2	structures					420:429	asymmetrical structures	407:429	asymmetrical structures commonly found in nature	407:454	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	2	34	located	found	440:444	arg1	nature					449:454	nature	449:454	nature	449:454	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
30532014	5	35	theme	points	965:970	arg1	feature					897:903	A key feature	891:903	A key feature of our approach	891:919	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	5	35	theme	points	965:970	arg1	installation					928:939	the installation	924:939	the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors	924:1047	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	0	36	theme	N-glycans	53:61	arg1	synthesis					32:40	the chemoenzymatic synthesis	13:40	the chemoenzymatic synthesis of complex N-glycans	13:61	Streamlining the chemoenzymatic synthesis of complex N-glycans by a stop and go strategy.					
30532014	4	37	gly	glycopeptide	651:662	arg2	glycopeptide					651:662	a readily available bi-antennary glycopeptide	618:662	a readily available bi-antennary glycopeptide	618:662	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	5	38	theme	recombinant	978:988	arg1	MGAT4					990:994	recombinant MGAT4	978:994	recombinant MGAT4	978:994	A key feature of our approach is the installation of additional branching points using recombinant MGAT4 and MGAT5 in combination with unnatural sugar donors.					
30532014	6	39	theme	enzymatic	1081:1089	arg1	synthesis					1091:1099	the enzymatic synthesis	1077:1099	the enzymatic synthesis	1077:1099	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	1	40	theme	multi-antennary	156:170	arg1	glycans					181:187	highly complex multi-antennary N-linked glycans	141:187	highly complex multi-antennary N-linked glycans	141:187	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	4	41	theme	bi-antennary	638:649	arg1	glycopeptide					651:662	a readily available bi-antennary glycopeptide	618:662	a readily available bi-antennary glycopeptide	618:662	Here we describe a biomimetic approach in which a readily available bi-antennary glycopeptide can be converted in ten or fewer chemical and enzymatic steps into multi-antennary N-glycans that at each arm can be uniquely extended by glycosyltransferases to give access to highly complex asymmetrically branched N-glycans.					
30532014	6	42	theme	natural	1160:1166	arg1	counterpart					1168:1178	their natural counterpart	1154:1178	their natural counterpart	1154:1178	At an appropriate point in the enzymatic synthesis, the unnatural monosaccharides can be converted into their natural counterpart, allowing each arm to be elaborated into a unique appendage.					
30532014	3	43	dep	perform	498:504	arg1	hampers					536:542	hampers	536:542	hampers progress in glycoscience	536:567	Only highly specialized laboratories can perform such syntheses, which greatly hampers progress in glycoscience.					
30532014	1	44	theme	complex	148:154	arg1	glycans					181:187	highly complex multi-antennary N-linked glycans	141:187	highly complex multi-antennary N-linked glycans	141:187	Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans.					
30532014	3	45	theme	specialized	469:479	arg1	laboratories					481:492	Only highly specialized laboratories	457:492	Only highly specialized laboratories	457:492	Only highly specialized laboratories can perform such syntheses, which greatly hampers progress in glycoscience.					
30532014	2	46	theme	branch-selective	375:390	arg1	manner					392:397	a branch-selective manner	373:397	a branch-selective manner	373:397	These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases in a branch-selective manner to give asymmetrical structures commonly found in nature.					
31819007	5	0	from	changes	798:804	arg1	glycolipids					809:819	glycolipids	809:819	glycolipids	809:819	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	0	from	changes	798:804	arg1	NSs					781:783	NSs	781:783	NSs	781:783	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	0	from	changes	798:804	arg1	glycoproteins					825:837	glycoproteins	825:837	glycoproteins	825:837	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	1	theme	receptor	899:906	arg1	subset					852:857	a subset	850:857	a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen	850:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	6	2	theme	galactose	1012:1020	arg1	levels					1034:1039	total sialic acid, galactose, and GalNAc levels	993:1039	total sialic acid, galactose, and GalNAc levels in glycans	993:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	0	3	theme	glycome	69:75	arg1	structure					77:85	cell-surface glycome structure	56:85	cell-surface glycome structure	56:85	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	8	4	from	role	1212:1215	arg1	signaling					1266:1274	death receptor signaling	1251:1274	death receptor signaling	1251:1274	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	5	5	theme	LC-MS-based	643:653	arg1	glycolipid					655:664	LC-MS-based glycolipid	643:664	LC-MS-based glycolipid	643:664	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	6	theme	FS-7-associated	908:922	arg1	antigen					932:938	FS-7-associated surface antigen	908:938	the cell-surface death receptor FS-7-associated surface antigen	876:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	6	7	from	levels	1034:1039	arg1	glycans					1044:1050	glycans	1044:1050	glycans	1044:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	4	8	theme	essential	586:594	arg1	NSs					596:598	essential NSs	586:598	essential NSs	586:598	To address this knowledge gap, here we examined UDP-galactose 4'-epimerase (GALE), which interconverts two pairs of essential NSs.					
31819007	5	9	dep	receptor	899:906	arg1	antigen					932:938	FS-7-associated surface antigen	908:938	the cell-surface death receptor FS-7-associated surface antigen	876:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	7	10	theme	ligand-induced	1165:1178	arg1	apoptosis					1180:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	2	11	theme	cell	323:326	arg1	physiology					328:337	normal cell physiology	316:337	normal cell physiology	316:337	In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are dysregulated in disease.					
31819007	5	12	theme	human	740:744	arg1	cells					746:750	human cells	740:750	human cells	740:750	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	8	13	theme	molecular	1310:1318	arg1	etiology					1320:1327	the molecular etiology	1306:1327	the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome	1306:1418	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	2	14	theme	NS	281:282	arg1	levels					284:289	NS levels	281:289	NS levels	281:289	In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are dysregulated in disease.					
31819007	6	15	theme	sialic	999:1004	arg1	acid					1006:1009	total sialic acid	993:1009	total sialic acid	993:1009	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	5	16	theme	flow	623:626	arg1	cytometry					628:636	flow cytometry	623:636	flow cytometry	623:636	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	7	17	theme	surface	1149:1155	arg1	apoptosis					1180:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	0	18	theme	Human	0:4	arg1	4'-epimerase					20:31	Human UDP-galactose 4'-epimerase	0:31	Human UDP-galactose 4'-epimerase (GALE)	0:38	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	0	18	theme	Human	0:4	arg1	GALE					34:37	GALE	34:37	GALE	34:37	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	1	19	theme	abundant	156:163	arg1	metabolites					165:175	abundant metabolites	156:175	abundant metabolites that serve as monosaccharide donors for glycosyltransferases	156:236	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	1	19	theme	abundant	156:163	arg1	sugars					142:147	nucleotide sugars	131:147	nucleotide sugars (NSs)	131:153	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	1	19	theme	abundant	156:163	arg1	donors					206:211	monosaccharide donors	191:211	monosaccharide donors for glycosyltransferases	191:236	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	0	20	theme	UDP-galactose	6:18	arg1	4'-epimerase					20:31	Human UDP-galactose 4'-epimerase	0:31	Human UDP-galactose 4'-epimerase (GALE)	0:38	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	0	20	theme	UDP-galactose	6:18	arg1	GALE					34:37	GALE	34:37	GALE	34:37	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	4	21	theme	knowledge	486:494	arg1	gap					496:498	this knowledge gap	481:498	this knowledge gap	481:498	To address this knowledge gap, here we examined UDP-galactose 4'-epimerase (GALE), which interconverts two pairs of essential NSs.					
31819007	7	22	theme	cell	1090:1093	arg1	signaling					1095:1103	cell signaling	1090:1103	cell signaling	1090:1103	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	2	23	theme	normal	316:321	arg1	physiology					328:337	normal cell physiology	316:337	normal cell physiology	316:337	In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are dysregulated in disease.					
31819007	3	24	theme	mammalian	385:393	arg1	cells					395:399	mammalian cells	385:399	mammalian cells	385:399	However, how mammalian cells regulate NS levels and pathway flux remains largely uncharacterized.					
31819007	6	25	theme	total	993:997	arg1	acid					1006:1009	total sialic acid	993:1009	total sialic acid	993:1009	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	8	26	theme	regulation	1237:1246	arg1	role					1212:1215	a role	1210:1215	a role of GALE-mediated NS regulation in death receptor signaling	1210:1274	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	8	27	theme	metabolic	1401:1409	arg1	syndrome					1411:1418	metabolic syndrome	1401:1418	metabolic syndrome	1401:1418	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	8	28	contain	have	1284:1287	arg2	implications					1289:1300	implications	1289:1300	implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome	1289:1418	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	8	28	contain	have	1284:1287	arg1	results					1195:1201	Our results	1191:1201	Our results	1191:1201	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	3	29	theme	pathway	424:430	arg1	flux					432:435	pathway flux	424:435	pathway flux	424:435	However, how mammalian cells regulate NS levels and pathway flux remains largely uncharacterized.					
31819007	8	30	theme	GALE-mediated	1220:1232	arg1	regulation					1237:1246	GALE-mediated NS regulation	1220:1246	GALE-mediated NS regulation	1220:1246	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	6	31	from	decreases	980:988	arg1	levels					1034:1039	total sialic acid, galactose, and GalNAc levels	993:1039	total sialic acid, galactose, and GalNAc levels in glycans	993:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	8	32	theme	NS	1359:1360	arg1	galactosemia					1384:1395	galactosemia	1384:1395	galactosemia	1384:1395	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	8	32	theme	NS	1359:1360	arg1	syndrome					1411:1418	metabolic syndrome	1401:1418	metabolic syndrome	1401:1418	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	8	32	theme	NS	1359:1360	arg1	imbalances					1362:1371	NS imbalances	1359:1371	NS imbalances	1359:1371	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	1	33	theme	Glycan	101:106	arg1	biosynthesis					108:119	Glycan biosynthesis	101:119	Glycan biosynthesis	101:119	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	2	34	from	fluctuations	265:276	arg1	levels					284:289	NS levels	281:289	NS levels	281:289	In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are dysregulated in disease.					
31819007	3	35	theme	NS	410:411	arg1	levels					413:418	NS levels	410:418	NS levels	410:418	However, how mammalian cells regulate NS levels and pathway flux remains largely uncharacterized.					
31819007	6	36	theme	substantial	968:978	arg1	decreases					980:988	substantial decreases	968:988	substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans	968:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	5	37	theme	CRISPR/Cas9-mediated	702:721	arg1	deletion					728:735	CRISPR/Cas9-mediated GALE deletion	702:735	CRISPR/Cas9-mediated GALE deletion in human cells	702:750	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	1	38	theme	monosaccharide	191:204	arg1	metabolites					165:175	abundant metabolites	156:175	abundant metabolites that serve as monosaccharide donors for glycosyltransferases	156:236	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	1	38	theme	monosaccharide	191:204	arg1	donors					206:211	monosaccharide donors	191:211	monosaccharide donors for glycosyltransferases	191:236	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	8	39	theme	receptor	1257:1264	arg1	signaling					1266:1274	death receptor signaling	1251:1274	death receptor signaling	1251:1274	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	5	40	theme	GALE	723:726	arg1	deletion					728:735	CRISPR/Cas9-mediated GALE deletion	702:735	CRISPR/Cas9-mediated GALE deletion in human cells	702:750	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	41	from	deletion	728:735	arg1	cells					746:750	human cells	740:750	human cells	740:750	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	42	theme	dramatic	789:796	arg1	changes					798:804	dramatic changes	789:804	dramatic changes in glycolipids and glycoproteins	789:837	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	4	43	theme	UDP-galactose	518:530	arg1	GALE					546:549	GALE	546:549	GALE	546:549	To address this knowledge gap, here we examined UDP-galactose 4'-epimerase (GALE), which interconverts two pairs of essential NSs.					
31819007	4	43	theme	UDP-galactose	518:530	arg1	4'-epimerase					532:543	UDP-galactose 4'-epimerase	518:543	UDP-galactose 4'-epimerase (GALE)	518:550	To address this knowledge gap, here we examined UDP-galactose 4'-epimerase (GALE), which interconverts two pairs of essential NSs.					
31819007	5	44	theme	glycan	670:675	arg1	profiling					677:685	glycan profiling	670:685	glycan profiling	670:685	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	45	gly	glycoproteins	825:837	arg1	glycoproteins					825:837	glycoproteins	825:837	glycoproteins	825:837	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	46	theme	surface	924:930	arg1	antigen					932:938	FS-7-associated surface antigen	908:938	the cell-surface death receptor FS-7-associated surface antigen	876:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	7	47	theme	antigen	1157:1163	arg1	apoptosis					1180:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	5	48	from	imbalances	767:776	arg1	glycolipids					809:819	glycolipids	809:819	glycolipids	809:819	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	48	from	imbalances	767:776	arg1	NSs					781:783	NSs	781:783	NSs	781:783	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	48	from	imbalances	767:776	arg1	glycoproteins					825:837	glycoproteins	825:837	glycoproteins	825:837	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	7	49	theme	FS-7-associated	1133:1147	arg1	apoptosis					1180:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	FS-7-associated surface antigen ligand-induced apoptosis	1133:1188	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	8	50	theme	NS	1234:1235	arg1	regulation					1237:1246	GALE-mediated NS regulation	1220:1246	GALE-mediated NS regulation	1220:1246	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	7	51	theme	GALE-/-	1109:1115	arg1	cells					1117:1121	GALE-/- cells	1109:1121	GALE-/- cells	1109:1121	These changes also directly impacted cell signaling, as GALE-/- cells exhibited FS-7-associated surface antigen ligand-induced apoptosis.					
31819007	6	52	theme	GalNAc	1027:1032	arg1	levels					1034:1039	total sialic acid, galactose, and GalNAc levels	993:1039	total sialic acid, galactose, and GalNAc levels in glycans	993:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	8	53	theme	illnesses	1332:1340	arg1	etiology					1320:1327	the molecular etiology	1306:1327	the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome	1306:1418	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	6	54	theme	acid	1006:1009	arg1	levels					1034:1039	total sialic acid, galactose, and GalNAc levels	993:1039	total sialic acid, galactose, and GalNAc levels in glycans	993:1050	In particular, we observed substantial decreases in total sialic acid, galactose, and GalNAc levels in glycans.					
31819007	2	55	theme	signal-dependent	248:263	arg1	fluctuations					265:276	signal-dependent fluctuations	248:276	signal-dependent fluctuations in NS levels	248:289	In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are dysregulated in disease.					
31819007	1	56	theme	nucleotide	131:140	arg1	metabolites					165:175	abundant metabolites	156:175	abundant metabolites that serve as monosaccharide donors for glycosyltransferases	156:236	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	1	56	theme	nucleotide	131:140	arg1	NSs					150:152	NSs	150:152	NSs	150:152	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	1	56	theme	nucleotide	131:140	arg1	sugars					142:147	nucleotide sugars	131:147	nucleotide sugars (NSs)	131:153	Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases.					
31819007	8	57	theme	death	1251:1255	arg1	signaling					1266:1274	death receptor signaling	1251:1274	death receptor signaling	1251:1274	Our results reveal a role of GALE-mediated NS regulation in death receptor signaling and may have implications for the molecular etiology of illnesses characterized by NS imbalances, including galactosemia and metabolic syndrome.					
31819007	4	58	theme	NSs	596:598	arg1	pairs					577:581	two pairs	573:581	two pairs of essential NSs	573:598	To address this knowledge gap, here we examined UDP-galactose 4'-epimerase (GALE), which interconverts two pairs of essential NSs.					
31819007	0	59	theme	cell-surface	56:67	arg1	structure					77:85	cell-surface glycome structure	56:85	cell-surface glycome structure	56:85	Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.					
31819007	5	60	theme	cell-surface	880:891	arg1	receptor					899:906	the cell-surface death receptor	876:906	the cell-surface death receptor FS-7-associated surface antigen	876:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	61	theme	integrins	862:870	arg1	subset					852:857	a subset	850:857	a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen	850:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	62	theme	major	761:765	arg1	imbalances					767:776	major imbalances	761:776	major imbalances in NSs	761:783	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
31819007	5	63	theme	death	893:897	arg1	receptor					899:906	the cell-surface death receptor	876:906	the cell-surface death receptor FS-7-associated surface antigen	876:938	Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profiling, we found that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramatic changes in glycolipids and glycoproteins, including a subset of integrins and the cell-surface death receptor FS-7-associated surface antigen.					
29615747	1	0	with	humans	180:185	arg1	ebolaviruses					199:210	Reston ebolaviruses	192:210	Reston ebolaviruses (REBOV) showing the lowest	192:237	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	1	0	with	humans	180:185	arg1	ZEBOV					263:267	ZEBOV	263:267	ZEBOV	263:267	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	1	0	with	humans	180:185	arg1	ebolaviruses					249:260	Zaire ebolaviruses	243:260	Zaire ebolaviruses (ZEBOV) showing the highest	243:288	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	1	0	with	humans	180:185	arg1	REBOV					213:217	REBOV	213:217	REBOV	213:217	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	3	1	theme	macrophage	504:513	arg1	MGL/CD301					551:559	MGL/CD301	551:559	MGL/CD301	551:559	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	1	theme	macrophage	504:513	arg1	lectin					543:548	macrophage galactose-type calcium-type lectin	504:548	macrophage galactose-type calcium-type lectin (MGL/CD301)	504:560	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	1	theme	macrophage	504:513	arg1	receptors					493:501	their counter receptors	479:501	their counter receptors	479:501	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	1	theme	macrophage	504:513	arg1	one					472:474	one	472:474	one	472:474	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	4	2	theme	GPs	780:782	arg1	acids					751:755	the N-terminal 18 amino acids	727:755	the N-terminal 18 amino acids (33rd through 50th) of GPs	727:782	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
29615747	4	3	theme	N-terminal	731:740	arg1	acids					751:755	the N-terminal 18 amino acids	727:755	the N-terminal 18 amino acids (33rd through 50th) of GPs	727:782	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
29615747	7	4	theme	extended	1084:1091	arg1	N-glycans					1093:1101	extended N-glycans	1084:1101	extended N-glycans on GPs	1084:1108	Therefore, these data strongly suggest that extended N-glycans on GPs reduce MGL/CD301-mediated viral infectivity by hindering the interaction between GPs and MGL/CD301 preferentially binds O-glycans.					
29615747	3	5	theme	glycoproteins	448:460	arg1	features					416:423	the structural features	401:423	the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301),	401:561	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	0	6	theme	Glycoprotein	41:52	arg1	Domain					11:16	A Critical Domain	0:16	A Critical Domain of Ebolavirus Envelope Glycoprotein	0:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	0	6	theme	Glycoprotein	41:52	arg1	Glycoprotein					41:52	Ebolavirus Envelope Glycoprotein	21:52	Ebolavirus Envelope Glycoprotein	21:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	3	7	dep	receptors	493:501	arg1	counter					485:491	counter	485:491	counter	485:491	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	1	8	theme	Reston	192:197	arg1	ebolaviruses					199:210	Reston ebolaviruses	192:210	Reston ebolaviruses (REBOV) showing the lowest	192:237	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	1	8	theme	Reston	192:197	arg1	REBOV					213:217	REBOV	213:217	REBOV	213:217	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	0	9	theme	Critical	2:9	arg1	Domain					11:16	A Critical Domain	0:16	A Critical Domain of Ebolavirus Envelope Glycoprotein	0:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	0	9	theme	Critical	2:9	arg1	Glycoprotein					41:52	Ebolavirus Envelope Glycoprotein	21:52	Ebolavirus Envelope Glycoprotein	21:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	6	10	theme	N-glycan	936:943	arg1	extension					945:953	N-glycan extension	936:953	N-glycan extension	936:953	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	5	11	from	REBOV	915:919	arg1	extended					903:910	extended	903:910	extended	903:910	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	3	12	theme	crucial	568:574	arg1	roles					576:580	crucial roles	568:580	crucial roles	568:580	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	13	theme	ebolavirus	428:437	arg1	glycoproteins					448:460	ebolavirus envelope glycoproteins	428:460	ebolavirus envelope glycoproteins (GPs)	428:466	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	13	theme	ebolavirus	428:437	arg1	GPs					463:465	GPs	463:465	GPs	463:465	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	2	14	theme	molecular	304:312	arg1	basis					314:318	the molecular basis	300:318	the molecular basis of this differential mortality/infectivity	300:361	However, the molecular basis of this differential mortality/infectivity remains unclear.					
29615747	3	15	theme	envelope	439:446	arg1	glycoproteins					448:460	ebolavirus envelope glycoproteins	428:460	ebolavirus envelope glycoproteins (GPs)	428:466	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	15	theme	envelope	439:446	arg1	GPs					463:465	GPs	463:465	GPs	463:465	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	5	16	from	extended	903:910	arg1	ZEBOV					929:933	ZEBOV	929:933	ZEBOV	929:933	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	5	16	from	extended	903:910	arg1	REBOV					915:919	REBOV	915:919	REBOV	915:919	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	4	17	theme	REBOV	639:643	arg1	infectivity					624:634	The low infectivity	616:634	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301	616:697	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
29615747	4	18	theme	amino	745:749	arg1	acids					751:755	the N-terminal 18 amino acids	727:755	the N-terminal 18 amino acids (33rd through 50th) of GPs	727:782	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
29615747	3	19	theme	one	472:474	arg1	features					416:423	the structural features	401:423	the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301),	401:561	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	0	20	theme	Envelope	32:39	arg1	Glycoprotein					41:52	Ebolavirus Envelope Glycoprotein	21:52	Ebolavirus Envelope Glycoprotein	21:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	3	21	theme	receptors	493:501	arg1	lectin					543:548	macrophage galactose-type calcium-type lectin	504:548	macrophage galactose-type calcium-type lectin (MGL/CD301)	504:560	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	21	theme	receptors	493:501	arg1	receptors					493:501	their counter receptors	479:501	their counter receptors	479:501	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	21	theme	receptors	493:501	arg1	one					472:474	one	472:474	one	472:474	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	6	22	theme	residues	1030:1037	arg1	replacement					975:985	the replacement	971:985	the replacement of aforementioned N-terminal 18 amino acid residues	971:1037	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	3	23	theme	viral	597:601	arg1	infectivity					603:613	viral infectivity	597:613	viral infectivity	597:613	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	5	24	theme	GPs	865:867	arg1	glycans					854:860	glycans	854:860	glycans of GPs	854:867	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	5	25	theme	structural	831:840	arg1	analysis					842:849	structural analysis	831:849	structural analysis of glycans of GPs	831:867	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	0	26	theme	Ebolavirus	21:30	arg1	Glycoprotein					41:52	Ebolavirus Envelope Glycoprotein	21:52	Ebolavirus Envelope Glycoprotein	21:52	A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity.					
29615747	6	27	theme	N-terminal	1005:1014	arg1	residues					1030:1037	aforementioned N-terminal 18 amino acid residues	990:1037	aforementioned N-terminal 18 amino acid residues	990:1037	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	6	28	theme	acid	1025:1028	arg1	residues					1030:1037	aforementioned N-terminal 18 amino acid residues	990:1037	aforementioned N-terminal 18 amino acid residues	990:1037	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	7	29	theme	MGL/CD301-mediated	1117:1134	arg1	infectivity					1142:1152	MGL/CD301-mediated viral infectivity	1117:1152	MGL/CD301-mediated viral infectivity	1117:1152	Therefore, these data strongly suggest that extended N-glycans on GPs reduce MGL/CD301-mediated viral infectivity by hindering the interaction between GPs and MGL/CD301 preferentially binds O-glycans.					
29615747	2	30	theme	mortality/infectivity	341:361	arg1	basis					314:318	the molecular basis	300:318	the molecular basis of this differential mortality/infectivity	300:361	However, the molecular basis of this differential mortality/infectivity remains unclear.					
29615747	3	31	theme	structural	405:414	arg1	features					416:423	the structural features	401:423	the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301),	401:561	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	2	32	theme	differential	328:339	arg1	mortality/infectivity					341:361	this differential mortality/infectivity	323:361	this differential mortality/infectivity	323:361	However, the molecular basis of this differential mortality/infectivity remains unclear.					
29615747	5	33	from	ZEBOV	929:933	arg1	extended					903:910	extended	903:910	extended	903:910	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	1	34	theme	varying	136:142	arg1	degrees					144:150	varying degrees	136:150	varying degrees of mortality/infectivity	136:175	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	6	35	theme	amino	1019:1023	arg1	residues					1030:1037	aforementioned N-terminal 18 amino acid residues	990:1037	aforementioned N-terminal 18 amino acid residues	990:1037	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	3	36	gly	glycoproteins	448:460	arg1	glycoproteins					448:460	ebolavirus envelope glycoproteins	428:460	ebolavirus envelope glycoproteins (GPs)	428:466	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	36	gly	glycoproteins	448:460	arg1	GPs					463:465	GPs	463:465	GPs	463:465	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	7	37	from	N-glycans	1093:1101	arg1	GPs					1106:1108	GPs	1106:1108	GPs	1106:1108	Therefore, these data strongly suggest that extended N-glycans on GPs reduce MGL/CD301-mediated viral infectivity by hindering the interaction between GPs and MGL/CD301 preferentially binds O-glycans.					
29615747	3	38	theme	calcium-type	530:541	arg1	MGL/CD301					551:559	MGL/CD301	551:559	MGL/CD301	551:559	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	38	theme	calcium-type	530:541	arg1	lectin					543:548	macrophage galactose-type calcium-type lectin	504:548	macrophage galactose-type calcium-type lectin (MGL/CD301)	504:560	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	38	theme	calcium-type	530:541	arg1	receptors					493:501	their counter receptors	479:501	their counter receptors	479:501	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	38	theme	calcium-type	530:541	arg1	one					472:474	one	472:474	one	472:474	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	4	39	dep	acids	751:755	arg1	50th					771:774	50th	771:774	50th	771:774	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
29615747	3	40	theme	galactose-type	515:528	arg1	MGL/CD301					551:559	MGL/CD301	551:559	MGL/CD301	551:559	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	40	theme	galactose-type	515:528	arg1	lectin					543:548	macrophage galactose-type calcium-type lectin	504:548	macrophage galactose-type calcium-type lectin (MGL/CD301)	504:560	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	40	theme	galactose-type	515:528	arg1	receptors					493:501	their counter receptors	479:501	their counter receptors	479:501	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	3	40	theme	galactose-type	515:528	arg1	one					472:474	one	472:474	one	472:474	Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial roles in determining viral infectivity.					
29615747	5	41	theme	glycans	854:860	arg1	analysis					842:849	structural analysis	831:849	structural analysis of glycans of GPs	831:867	Furthermore, structural analysis of glycans of GPs revealed that N-glycans were more extended in REBOV than in ZEBOV.					
29615747	1	42	theme	Zaire	243:247	arg1	ZEBOV					263:267	ZEBOV	263:267	ZEBOV	263:267	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	1	42	theme	Zaire	243:247	arg1	ebolaviruses					249:260	Zaire ebolaviruses	243:260	Zaire ebolaviruses (ZEBOV) showing the highest	243:288	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	7	43	theme	viral	1136:1140	arg1	infectivity					1142:1152	MGL/CD301-mediated viral infectivity	1117:1152	MGL/CD301-mediated viral infectivity	1117:1152	Therefore, these data strongly suggest that extended N-glycans on GPs reduce MGL/CD301-mediated viral infectivity by hindering the interaction between GPs and MGL/CD301 preferentially binds O-glycans.					
29615747	6	44	theme	aforementioned	990:1003	arg1	residues					1030:1037	aforementioned N-terminal 18 amino acid residues	990:1037	aforementioned N-terminal 18 amino acid residues	990:1037	N-glycan extension was reversed by the replacement of aforementioned N-terminal 18 amino acid residues.					
29615747	1	45	theme	mortality/infectivity	155:175	arg1	degrees					144:150	varying degrees	136:150	varying degrees of mortality/infectivity	136:175	Ebolaviruses comprises 5 species that exert varying degrees of mortality/infectivity in humans with Reston ebolaviruses (REBOV) showing the lowest and Zaire ebolaviruses (ZEBOV) showing the highest.					
29615747	4	46	theme	low	620:622	arg1	infectivity					624:634	The low infectivity	616:634	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301	616:697	The low infectivity of REBOV mediated by the interaction between GPs and MGL/CD301 dramatically increased when the N-terminal 18 amino acids (33rd through 50th) of GPs were replaced with that of ZEBOV.					
31884067	8	0	theme	responsible	1181:1191	arg1	B3gat1					1214:1219	B3gat1	1214:1219	B3gat1	1214:1219	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	8	0	theme	responsible	1181:1191	arg1	glycosyltransferase					1193:1211	the responsible glycosyltransferase	1177:1211	the responsible glycosyltransferase (B3gat1)	1177:1220	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	6	1	theme	glycan	939:944	arg1	expression					919:928	expression	919:928	expression of HNK-1 glycan	919:944	RESULTS Among several glycans, expression of HNK-1 glycan was strikingly upregulated in the PRMT1-deficient cerebellum.					
31884067	11	2	theme	novel	1707:1711	arg1	PRMT1					1696:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	11	2	theme	novel	1707:1711	arg1	regulator					1713:1721	a novel regulator	1705:1721	a novel regulator of HNK-1 glycan production in the cerebellum	1705:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	2	3	theme	brain-specific	289:302	arg1	glycans					304:310	these brain-specific glycans	283:310	these brain-specific glycans	283:310	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	12	4	theme	arginine	1826:1833	arg1	methylation					1835:1845	arginine methylation	1826:1845	arginine methylation	1826:1845	Mechanisms involving crosstalk between glycosylation and arginine methylation are suggested to occur.					
31884067	4	5	theme	protein	677:683	arg1	PRMT1					715:719	PRMT1	715:719	PRMT1	715:719	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	5	theme	protein	677:683	arg1	methyltransferase					694:710	protein arginine methyltransferase 1	677:712	protein arginine methyltransferase 1 (PRMT1)	677:720	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	6	theme	methyltransferase	694:710	arg1	knockout					665:672	knockout	665:672	knockout	665:672	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	8	7	dep	level	1155:1159	arg1	the					1146:1148	the	1146:1148	the	1146:1148	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	5	8	theme	western	870:876	arg1	blotting					878:885	western blotting	870:885	western blotting	870:885	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	10	9	theme	carrier	1652:1658	arg1	glycoproteins					1660:1672	carrier glycoproteins	1652:1672	carrier glycoproteins	1652:1672	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	11	10	from	regulator	1713:1721	arg1	cerebellum					1757:1766	the cerebellum	1753:1766	the cerebellum	1753:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	5	11	theme	quantitative	846:857	arg1	RT-PCR					859:864	quantitative RT-PCR	846:864	quantitative RT-PCR	846:864	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	6	12	theme	PRMT1-deficient	980:994	arg1	cerebellum					996:1005	the PRMT1-deficient cerebellum	976:1005	the PRMT1-deficient cerebellum	976:1005	RESULTS Among several glycans, expression of HNK-1 glycan was strikingly upregulated in the PRMT1-deficient cerebellum.					
31884067	10	13	theme	enzyme	1641:1646	arg1	upregulation					1608:1619	upregulation	1608:1619	upregulation of the biosynthetic enzyme	1608:1646	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	10	13	theme	enzyme	1641:1646	arg1	glycoproteins					1660:1672	carrier glycoproteins	1652:1672	carrier glycoproteins	1652:1672	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	9	14	theme	HNK-1	1302:1306	arg1	glycoproteins					1316:1328	HNK-1 carrier glycoproteins	1302:1328	HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2	1302:1409	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	14	theme	HNK-1	1302:1306	arg1	L1					1376:1377	L1	1376:1377	L1	1376:1377	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	14	theme	HNK-1	1302:1306	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	14	theme	HNK-1	1302:1306	arg1	subunit					1397:1403	AMPA receptor subunit	1383:1403	AMPA receptor subunit	1383:1403	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	1	15	theme	natural	146:152	arg1	HNK-1					164:168	HNK-1	164:168	HNK-1	164:168	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	1	15	theme	natural	146:152	arg1	killer-1					154:161	human natural killer-1	140:161	human natural killer-1 (HNK-1)	140:169	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	5	16	theme	responsible	786:796	arg1	glycosyltransferases					765:784	glycosyltransferases	765:784	glycosyltransferases responsible for HNK-1	765:806	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	9	17	theme	glycoproteins	1316:1328	arg1	expression					1288:1297	the expression	1284:1297	the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2	1284:1409	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	2	18	theme	regulatory	244:253	arg1	mechanisms					255:264	the regulatory mechanisms	240:264	the regulatory mechanisms of expression of these brain-specific glycans	240:310	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	2	18	theme	regulatory	244:253	arg1	unknown					324:330	unknown	324:330	unknown	324:330	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	11	19	from	cerebellum	1757:1766	arg1	PRMT1					1696:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	11	19	from	cerebellum	1757:1766	arg1	regulator					1713:1721	a novel regulator	1705:1721	a novel regulator of HNK-1 glycan production in the cerebellum	1705:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	4	20	theme	several	533:539	arg1	epitope					579:585	the HNK-1 epitope	569:585	the HNK-1 epitope	569:585	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	20	theme	several	533:539	arg1	types					541:545	several types	533:545	several types	533:545	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	10	21	theme	CONCLUSIONS	1466:1476	arg1	Loss					1478:1481	CONCLUSIONS Loss	1466:1481	CONCLUSIONS Loss of arginine methylation	1466:1505	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	10	22	gly	glycoproteins	1660:1672	arg1	glycoproteins					1660:1672	carrier glycoproteins	1652:1672	carrier glycoproteins	1652:1672	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	5	23	theme	expression	744:753	arg1	levels					755:760	the expression levels	740:760	the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins	740:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	9	24	theme	cell	1345:1348	arg1	NCAM					1369:1372	NCAM	1369:1372	NCAM	1369:1372	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	24	theme	cell	1345:1348	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	3	25	theme	modification	399:410	arg1	methylation					362:372	arginine methylation	353:372	arginine methylation	353:372	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	3	25	theme	modification	399:410	arg1	type					383:386	another type	375:386	another type of protein modification essential for neural development	375:443	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	10	26	theme	methylation	1495:1505	arg1	Loss					1478:1481	CONCLUSIONS Loss	1466:1481	CONCLUSIONS Loss of arginine methylation	1466:1505	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	4	27	from	mice	631:634	arg1	cerebellum					595:604	cerebellum	595:604	cerebellum	595:604	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	27	from	mice	631:634	arg1	cortex					619:624	cerebral cortex	610:624	cerebral cortex	610:624	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	27	from	mice	631:634	arg1	epitope					579:585	the HNK-1 epitope	569:585	the HNK-1 epitope	569:585	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	27	from	mice	631:634	arg1	types					541:545	several types	533:545	several types	533:545	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	0	28	theme	Region-specific	0:14	arg1	upregulation					16:27	Region-specific upregulation	0:27	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain	0:72	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain.					
31884067	4	29	dep	METHODS	513:519	arg1	analyzed					524:531	analyzed	524:531	analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1)	524:720	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	10	30	theme	HNK-1	1531:1535	arg1	glycan					1537:1542	HNK-1 glycan	1531:1542	HNK-1 glycan	1531:1542	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	11	31	theme	production	1739:1748	arg1	PRMT1					1696:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	11	31	theme	production	1739:1748	arg1	regulator					1713:1721	a novel regulator	1705:1721	a novel regulator of HNK-1 glycan production in the cerebellum	1705:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	3	32	theme	various	472:478	arg1	glycans					480:486	various glycans	472:486	various glycans	472:486	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	0	33	theme	glycan	38:43	arg1	upregulation					16:27	Region-specific upregulation	0:27	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain	0:72	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain.					
31884067	9	34	dep	molecule	1359:1366	arg1	GluA2					1405:1409	GluA2	1405:1409	GluA2	1405:1409	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	10	35	theme	developing	1551:1560	arg1	cerebellum					1562:1571	the developing cerebellum	1547:1571	the developing cerebellum	1547:1571	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	11	36	theme	HNK-1	1726:1730	arg1	production					1739:1748	HNK-1 glycan production	1726:1748	HNK-1 glycan production in the cerebellum	1726:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	5	37	theme	carrier	821:827	arg1	glycoproteins					829:841	HNK-1 carrier glycoproteins	815:841	HNK-1 carrier glycoproteins	815:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	9	38	theme	receptor	1388:1395	arg1	subunit					1397:1403	AMPA receptor subunit	1383:1403	AMPA receptor subunit	1383:1403	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	3	39	theme	developing	495:504	arg1	brain					506:510	the developing brain	491:510	the developing brain	491:510	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	4	40	theme	cerebral	610:617	arg1	cortex					619:624	cerebral cortex	610:624	cerebral cortex	610:624	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	0	41	theme	PRMT1-deficient	52:66	arg1	brain					68:72	the PRMT1-deficient brain	48:72	the PRMT1-deficient brain	48:72	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain.					
31884067	6	42	dep	RESULTS	888:894	arg1	glycans					910:916	several glycans	902:916	several glycans	902:916	RESULTS Among several glycans, expression of HNK-1 glycan was strikingly upregulated in the PRMT1-deficient cerebellum.					
31884067	4	43	from	types	541:545	arg1	cerebellum					595:604	cerebellum	595:604	cerebellum	595:604	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	43	from	types	541:545	arg1	cortex					619:624	cerebral cortex	610:624	cerebral cortex	610:624	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	43	from	types	541:545	arg1	mice					631:634	mice	631:634	mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1)	631:720	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	11	44	theme	GENERAL	1675:1681	arg1	PRMT1					1696:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	11	44	theme	GENERAL	1675:1681	arg1	regulator					1713:1721	a novel regulator	1705:1721	a novel regulator of HNK-1 glycan production in the cerebellum	1705:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	2	45	theme	glycans	304:310	arg1	expression					269:278	expression	269:278	expression of these brain-specific glycans	269:310	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	9	46	theme	PRMT1-deficient	1438:1452	arg1	cerebellum					1454:1463	the PRMT1-deficient cerebellum	1434:1463	the PRMT1-deficient cerebellum	1434:1463	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	8	47	theme	glycosyltransferase	1193:1211	arg1	activity					1165:1172	activity	1165:1172	activity	1165:1172	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	8	47	theme	glycosyltransferase	1193:1211	arg1	level					1155:1159	mRNA level	1150:1159	mRNA level	1150:1159	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	6	48	theme	HNK-1	933:937	arg1	glycan					939:944	HNK-1 glycan	933:944	HNK-1 glycan	933:944	RESULTS Among several glycans, expression of HNK-1 glycan was strikingly upregulated in the PRMT1-deficient cerebellum.					
31884067	10	49	theme	cerebral	1588:1595	arg1	cortex					1597:1602	the cerebral cortex	1584:1602	the cerebral cortex	1584:1602	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	10	50	theme	biosynthetic	1628:1639	arg1	enzyme					1641:1646	the biosynthetic enzyme	1624:1646	the biosynthetic enzyme	1624:1646	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	4	51	theme	arginine	685:692	arg1	PRMT1					715:719	PRMT1	715:719	PRMT1	715:719	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	51	theme	arginine	685:692	arg1	methyltransferase					694:710	protein arginine methyltransferase 1	677:712	protein arginine methyltransferase 1 (PRMT1)	677:720	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	52	dep	cerebellum	595:604	arg1	the					591:593	the	591:593	the	591:593	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	53	with	mice	631:634	arg1	knockout					665:672	knockout	665:672	knockout	665:672	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	1	54	theme	high-order	209:218	arg1	functions					220:228	high-order functions	209:228	high-order functions	209:228	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	8	55	theme	knockout	1243:1250	arg1	cerebellum					1252:1261	the knockout cerebellum	1239:1261	the knockout cerebellum	1239:1261	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	7	56	theme	cerebral	1082:1089	arg1	cortex					1091:1096	the cerebral cortex	1078:1096	the cerebral cortex	1078:1096	Furthermore, such upregulation was found in the cerebellum but not in the cerebral cortex.					
31884067	9	57	gly	glycoproteins	1316:1328	arg1	glycoproteins					1316:1328	HNK-1 carrier glycoproteins	1302:1328	HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2	1302:1409	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	57	gly	glycoproteins	1316:1328	arg1	L1					1376:1377	L1	1376:1377	L1	1376:1377	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	57	gly	glycoproteins	1316:1328	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	57	gly	glycoproteins	1316:1328	arg1	subunit					1397:1403	AMPA receptor subunit	1383:1403	AMPA receptor subunit	1383:1403	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	2	58	theme	expression	269:278	arg1	mechanisms					255:264	the regulatory mechanisms	240:264	the regulatory mechanisms of expression of these brain-specific glycans	240:310	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	2	58	theme	expression	269:278	arg1	unknown					324:330	unknown	324:330	unknown	324:330	However, the regulatory mechanisms of expression of these brain-specific glycans are largely unknown.					
31884067	1	59	theme	human	140:144	arg1	HNK-1					164:168	HNK-1	164:168	HNK-1	164:168	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	1	59	theme	human	140:144	arg1	killer-1					154:161	human natural killer-1	140:161	human natural killer-1 (HNK-1)	140:169	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	10	60	from	increase	1519:1526	arg1	cerebellum					1562:1571	the developing cerebellum	1547:1571	the developing cerebellum	1547:1571	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	10	60	from	increase	1519:1526	arg1	cortex					1597:1602	the cerebral cortex	1584:1602	the cerebral cortex	1584:1602	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	10	60	from	increase	1519:1526	arg1	glycan					1537:1542	HNK-1 glycan	1531:1542	HNK-1 glycan	1531:1542	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	5	61	theme	glycosyltransferases	765:784	arg1	levels					755:760	the expression levels	740:760	the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins	740:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	9	62	theme	carrier	1308:1314	arg1	glycoproteins					1316:1328	HNK-1 carrier glycoproteins	1302:1328	HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2	1302:1409	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	62	theme	carrier	1308:1314	arg1	L1					1376:1377	L1	1376:1377	L1	1376:1377	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	62	theme	carrier	1308:1314	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	62	theme	carrier	1308:1314	arg1	subunit					1397:1403	AMPA receptor subunit	1383:1403	AMPA receptor subunit	1383:1403	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	3	63	theme	arginine	353:360	arg1	methylation					362:372	arginine methylation	353:372	arginine methylation	353:372	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	3	63	theme	arginine	353:360	arg1	type					383:386	another type	375:386	another type of protein modification essential for neural development	375:443	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	0	64	from	upregulation	16:27	arg1	brain					68:72	the PRMT1-deficient brain	48:72	the PRMT1-deficient brain	48:72	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain.					
31884067	9	65	theme	neural	1338:1343	arg1	NCAM					1369:1372	NCAM	1369:1372	NCAM	1369:1372	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	65	theme	neural	1338:1343	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	7	66	theme	such	1021:1024	arg1	upregulation					1026:1037	such upregulation	1021:1037	such upregulation	1021:1037	Furthermore, such upregulation was found in the cerebellum but not in the cerebral cortex.					
31884067	9	67	theme	adhesion	1350:1357	arg1	NCAM					1369:1372	NCAM	1369:1372	NCAM	1369:1372	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	9	67	theme	adhesion	1350:1357	arg1	molecule					1359:1366	neural cell adhesion molecule	1338:1366	neural cell adhesion molecule (NCAM)	1338:1373	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	1	68	theme	BACKGROUND	75:84	arg1	Brains					86:91	BACKGROUND Brains	75:91	BACKGROUND Brains	75:91	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	11	69	from	production	1739:1748	arg1	cerebellum					1757:1766	the cerebellum	1753:1766	the cerebellum	1753:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	5	70	theme	glycoproteins	829:841	arg1	levels					755:760	the expression levels	740:760	the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins	740:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	3	71	theme	essential	412:420	arg1	modification					399:410	protein modification	391:410	protein modification essential for neural development	391:443	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	4	72	theme	HNK-1	573:577	arg1	epitope					579:585	the HNK-1 epitope	569:585	the HNK-1 epitope	569:585	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	10	73	theme	arginine	1486:1493	arg1	methylation					1495:1505	arginine methylation	1486:1505	arginine methylation	1486:1505	CONCLUSIONS Loss of arginine methylation leads to an increase in HNK-1 glycan in the developing cerebellum but not in the cerebral cortex via upregulation of the biosynthetic enzyme and carrier glycoproteins.					
31884067	0	74	theme	HNK-1	32:36	arg1	glycan					38:43	HNK-1 glycan	32:43	HNK-1 glycan	32:43	Region-specific upregulation of HNK-1 glycan in the PRMT1-deficient brain.					
31884067	1	75	theme	unique	114:119	arg1	killer-1					154:161	human natural killer-1	140:161	human natural killer-1 (HNK-1)	140:169	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	1	75	theme	unique	114:119	arg1	glycans					121:127	structurally unique glycans	101:127	structurally unique glycans	101:127	BACKGROUND Brains express structurally unique glycans, including human natural killer-1 (HNK-1), which participate in development and high-order functions.					
31884067	3	76	theme	protein	391:397	arg1	modification					399:410	protein modification	391:410	protein modification essential for neural development	391:443	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	11	77	theme	glycan	1732:1737	arg1	production					1739:1748	HNK-1 glycan production	1726:1748	HNK-1 glycan production in the cerebellum	1726:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	6	78	theme	several	902:908	arg1	glycans					910:916	several glycans	902:916	several glycans	902:916	RESULTS Among several glycans, expression of HNK-1 glycan was strikingly upregulated in the PRMT1-deficient cerebellum.					
31884067	4	79	theme	glycans	550:556	arg1	epitope					579:585	the HNK-1 epitope	569:585	the HNK-1 epitope	569:585	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	4	79	theme	glycans	550:556	arg1	types					541:545	several types	533:545	several types	533:545	METHODS We analyzed several types of glycans, including the HNK-1 epitope, in the cerebellum and cerebral cortex from mice with nervous system-specific knockout of protein arginine methyltransferase 1 (PRMT1).					
31884067	5	80	gly	glycoproteins	829:841	arg1	glycoproteins					829:841	HNK-1 carrier glycoproteins	815:841	HNK-1 carrier glycoproteins	815:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	3	81	theme	glycans	480:486	arg1	expression					458:467	the expression	454:467	the expression of various glycans in the developing brain	454:510	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	5	82	theme	HNK-1	815:819	arg1	glycoproteins					829:841	HNK-1 carrier glycoproteins	815:841	HNK-1 carrier glycoproteins	815:841	We also analyzed the expression levels of glycosyltransferases responsible for HNK-1 and of HNK-1 carrier glycoproteins by quantitative RT-PCR and western blotting.					
31884067	9	83	theme	AMPA	1383:1386	arg1	subunit					1397:1403	AMPA receptor subunit	1383:1403	AMPA receptor subunit	1383:1403	We also showed that the expression of HNK-1 carrier glycoproteins such as neural cell adhesion molecule (NCAM), L1 and AMPA receptor subunit GluA2 were also increased in the PRMT1-deficient cerebellum.					
31884067	8	84	theme	mRNA	1150:1153	arg1	level					1155:1159	mRNA level	1150:1159	mRNA level	1150:1159	Regarding the mechanisms, we demonstrated that the mRNA level and activity of the responsible glycosyltransferase (B3gat1) were elevated in the knockout cerebellum.					
31884067	3	85	theme	neural	426:431	arg1	development					433:443	neural development	426:443	neural development	426:443	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
31884067	7	86	located	found	1043:1047	arg1	cerebellum					1056:1065	the cerebellum	1052:1065	the cerebellum	1052:1065	Furthermore, such upregulation was found in the cerebellum but not in the cerebral cortex.					
31884067	7	86	located	found	1043:1047	arg2	upregulation					1026:1037	such upregulation	1021:1037	such upregulation	1021:1037	Furthermore, such upregulation was found in the cerebellum but not in the cerebral cortex.					
31884067	11	87	theme	SIGNIFICANCE	1683:1694	arg1	PRMT1					1696:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1	1675:1700	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	11	87	theme	SIGNIFICANCE	1683:1694	arg1	regulator					1713:1721	a novel regulator	1705:1721	a novel regulator of HNK-1 glycan production in the cerebellum	1705:1766	GENERAL SIGNIFICANCE PRMT1 is a novel regulator of HNK-1 glycan production in the cerebellum.					
31884067	3	88	from	expression	458:467	arg1	brain					506:510	the developing brain	491:510	the developing brain	491:510	We examined whether arginine methylation, another type of protein modification essential for neural development, impacts the expression of various glycans in the developing brain.					
30813247	9	0	theme	CHO	1480:1482	arg1	"					1510:1510	"Shingrix situation"	1491:1510	"Shingrix situation"	1491:1510	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	9	0	theme	CHO	1480:1482	arg1	cells					1484:1488	CHO cells	1480:1488	CHO cells ("Shingrix situation")	1480:1511	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	10	1	theme	decreased	1891:1899	arg1	number					1901:1906	a decreased number	1889:1906	a decreased number of interfering O-linked glycans	1889:1938	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	10	2	theme	epitopes	1699:1706	arg1	screening					1664:1672	screening	1664:1672	screening	1664:1672	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	3	3	theme	T	481:481	arg1	cell					483:486	virus-specific CD4+ T cell	461:486	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	4	4	theme	single	789:794	arg1	glycoprotein					802:813	only one single viral glycoprotein	780:813	only one single viral glycoprotein	780:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	10	5	theme	synthetic	1723:1731	arg1	library					1741:1747	a synthetic peptide library	1721:1747	a synthetic peptide library against serum samples from VZV-seropositive individuals	1721:1803	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	3	6	theme	antibody	508:515	arg1	response					524:531	antibody B cell response	508:531	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	4	7	theme	subunit	755:761	arg1	vaccine					763:769	a subunit vaccine	753:769	a subunit vaccine based on only one single viral glycoprotein	753:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	11	8	theme	subunit	2138:2144	arg1	vaccines					2146:2153	subunit vaccines	2138:2153	subunit vaccines	2138:2153	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	6	9	theme	Shingrix®	1051:1059	arg1	gE					1045:1046	the recombinant gE	1029:1046	the recombinant gE of Shingrix®	1029:1059	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	10	10	theme	O-linked	1923:1930	arg1	glycans					1932:1938	interfering O-linked glycans	1911:1938	interfering O-linked glycans	1911:1938	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	2	11	gly	glycoprotein	274:285	arg1	glycoprotein					274:285	glycoprotein E	274:287	glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation	274:421	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	4	12	theme	viral	796:800	arg1	glycoprotein					802:813	only one single viral glycoprotein	780:813	only one single viral glycoprotein	780:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	10	13	theme	serum	1757:1761	arg1	samples					1763:1769	serum samples	1757:1769	serum samples from VZV-seropositive individuals	1757:1803	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	9	14	dep	fibroblasts	1579:1589	arg1	"					1611:1611	Zostavax situation"	1593:1611	"Zostavax situation"	1592:1611	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	3	15	theme	cell	519:522	arg1	response					524:531	antibody B cell response	508:531	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	11	16	theme	higher	2049:2054	arg1	efficacy					2067:2074	the higher protective efficacy	2045:2074	the higher protective efficacy of Shingrix®	2045:2087	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	6	17	theme	CHO	1100:1102	arg1	cells					1105:1109	Chinese hamster ovary (CHO) cells	1077:1109	Chinese hamster ovary (CHO) cells	1077:1109	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	4	18	theme	current	647:653	arg1	notion					655:660	the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein	643:813	the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein	643:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	8	19	theme	gE	1305:1306	arg1	positions					1280:1288	positions	1280:1288	positions	1280:1288	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	8	19	theme	gE	1305:1306	arg1	structures					1265:1274	the glycan structures	1254:1274	the glycan structures	1254:1274	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	6	20	theme	ovary	1093:1097	arg1	cells					1105:1109	Chinese hamster ovary (CHO) cells	1077:1109	Chinese hamster ovary (CHO) cells	1077:1109	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	10	21	theme	specific	1954:1961	arg1	glycans					1972:1978	specific O-linked glycans	1954:1978	specific O-linked glycans enhancing antibody binding	1954:2005	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	3	22	theme	live	571:574	arg1	Zostavax®					596:604	Zostavax®	596:604	Zostavax®	596:604	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	3	22	theme	live	571:574	arg1	vaccine					587:593	the currently used live attenuated vaccine	552:593	the currently used live attenuated vaccine (Zostavax®)	552:605	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	8	23	dep	defining	1245:1252	arg1	i					1242:1242	i	1242:1242	i	1242:1242	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	10	24	link	O-linked	1963:1970	arg1	glycans					1972:1978	specific O-linked glycans	1954:1978	specific O-linked glycans enhancing antibody binding	1954:2005	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	6	25	theme	Chinese	1077:1083	arg1	cells					1105:1109	Chinese hamster ovary (CHO) cells	1077:1109	Chinese hamster ovary (CHO) cells	1077:1109	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	0	26	theme	Glycan-Peptide	62:75	arg1	Motifs					77:82	Glycan-Peptide Motifs	62:82	Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures	62:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	4	27	gly	glycoprotein	802:813	arg1	glycoprotein					802:813	only one single viral glycoprotein	780:813	only one single viral glycoprotein	780:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	9	28	theme	human	1573:1577	arg1	fibroblasts					1579:1589	human fibroblasts	1573:1589	human fibroblasts ("Zostavax situation")	1573:1612	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	10	29	theme	antibody	1990:1997	arg1	binding					1999:2005	antibody binding	1990:2005	antibody binding	1990:2005	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	8	30	theme	possible	1329:1336	arg1	features					1338:1345	possible features	1329:1345	possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®	1329:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	11	31	theme	enveloped	2164:2172	arg1	viruses					2174:2180	other enveloped viruses	2158:2180	other enveloped viruses	2158:2180	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	10	32	theme	glycan	1833:1838	arg1	signature					1840:1848	the O-linked glycan signature	1820:1848	the O-linked glycan signature	1820:1848	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	0	33	theme	B	98:98	arg1	Epitopes					105:112	B Cell Epitopes	98:112	B Cell Epitopes into Discrete Immunological Signatures	98:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	7	34	dep	occupancy	1130:1138	arg1	the					1119:1121	the	1119:1121	the	1119:1121	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	9	35	theme	Zostavax	1593:1600	arg1	"					1611:1611	Zostavax situation"	1593:1611	"Zostavax situation"	1592:1611	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	4	36	theme	virus	702:706	arg1	infection					708:716	an active virus infection	692:716	an active virus infection	692:716	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	2	37	theme	O-linked	400:407	arg1	glycosylation					409:421	O-linked glycosylation	400:421	O-linked glycosylation	400:421	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	8	38	theme	recombinant	1354:1364	arg1	pattern					1385:1391	the recombinant gE O-glycosylation pattern	1350:1391	the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®	1350:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	4	39	theme	live	670:673	arg1	vaccine					675:681	a live vaccine	668:681	a live vaccine	668:681	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	4	39	theme	live	670:673	arg1	efficient					738:746	efficient	738:746	efficient	738:746	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	2	40	theme	expressed	347:355	arg1	protein					357:363	the most abundantly expressed protein	327:363	the most abundantly expressed protein of VZV	327:370	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	40	theme	expressed	347:355	arg1	virus					314:318	varicella zoster virus	297:318	varicella zoster virus (VZV)	297:324	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	0	41	theme	Recombinant	0:10	arg1	E					25:25	Recombinant Glycoprotein E	0:25	Recombinant Glycoprotein E of Varicella Zoster Virus	0:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	9	42	theme	Shingrix	1492:1499	arg1	"					1510:1510	"Shingrix situation"	1491:1510	"Shingrix situation"	1491:1510	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	9	42	theme	Shingrix	1492:1499	arg1	cells					1484:1488	CHO cells	1480:1488	CHO cells ("Shingrix situation")	1480:1511	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	8	43	theme	O-glycosylation	1369:1383	arg1	pattern					1385:1391	the recombinant gE O-glycosylation pattern	1350:1391	the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®	1350:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	0	44	theme	Varicella	30:38	arg1	Virus					47:51	Varicella Zoster Virus	30:51	Varicella Zoster Virus	30:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	9	45	theme	glycan	1631:1636	arg1	occupancy					1643:1651	glycan site occupancy	1631:1651	glycan site occupancy	1631:1651	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	3	46	theme	stronger	452:459	arg1	response					488:495	stronger virus-specific CD4+ T cell response	452:495	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	0	47	theme	Virus	47:51	arg1	E					25:25	Recombinant Glycoprotein E	0:25	Recombinant Glycoprotein E of Varicella Zoster Virus	0:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	7	48	theme	glycan	1144:1149	arg1	structures					1151:1160	glycan structures	1144:1160	glycan structures	1144:1160	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	10	49	theme	antibodies	1874:1883	arg1	binding					1859:1865	binding	1859:1865	binding of IgG antibodies	1859:1883	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	10	50	theme	immunodominant	1677:1690	arg1	epitopes					1699:1706	immunodominant B cell epitopes	1677:1706	immunodominant B cell epitopes of gE	1677:1712	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	2	51	theme	virus	314:318	arg1	E					287:287	glycoprotein E	274:287	glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation	274:421	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	51	theme	virus	314:318	arg1	gE					290:291	gE	290:291	gE	290:291	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	5	52	theme	O-linked	899:906	arg1	glycans					908:914	numerous clustered O-linked glycans	880:914	numerous clustered O-linked glycans	880:914	We previously found gE to be heavily glycosylated, not least by numerous clustered O-linked glycans, when it was produced in human fibroblasts.					
30813247	9	53	link	O-linked	1543:1550	arg1	glycans					1552:1558	O-linked glycans	1543:1558	O-linked glycans than gE from human fibroblasts ("Zostavax situation")	1543:1612	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	8	54	theme	Shingrix®	1433:1441	arg1	efficacy					1421:1428	the vaccine efficacy	1409:1428	the vaccine efficacy of Shingrix®	1409:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	3	55	theme	cell	483:486	arg1	response					488:495	stronger virus-specific CD4+ T cell response	452:495	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	3	56	theme	CD4+	476:479	arg1	cell					483:486	virus-specific CD4+ T cell	461:486	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	10	57	theme	cell	1694:1697	arg1	epitopes					1699:1706	immunodominant B cell epitopes	1677:1706	immunodominant B cell epitopes of gE	1677:1712	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	0	58	contain	Contains	53:60	arg1	E					25:25	Recombinant Glycoprotein E	0:25	Recombinant Glycoprotein E of Varicella Zoster Virus	0:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	0	58	contain	Contains	53:60	arg2	Motifs					77:82	Glycan-Peptide Motifs	62:82	Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures	62:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	8	59	dep	identifying	1317:1327	arg1	ii					1313:1314	ii	1313:1314	ii	1313:1314	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	10	60	theme	glycans	1932:1938	arg1	number					1901:1906	a decreased number	1889:1906	a decreased number of interfering O-linked glycans	1889:1938	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	11	61	theme	protective	2056:2065	arg1	efficacy					2067:2074	the higher protective efficacy	2045:2074	the higher protective efficacy of Shingrix®	2045:2087	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	4	62	with	variance	629:636	arg1	notion					655:660	the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein	643:813	the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein	643:813	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	2	63	theme	glycoprotein	274:285	arg1	E					287:287	glycoprotein E	274:287	glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation	274:421	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	63	theme	glycoprotein	274:285	arg1	gE					290:291	gE	290:291	gE	290:291	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	9	64	theme	situation	1501:1509	arg1	"					1510:1510	"Shingrix situation"	1491:1510	"Shingrix situation"	1491:1510	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	9	64	theme	situation	1501:1509	arg1	cells					1484:1488	CHO cells	1480:1488	CHO cells ("Shingrix situation")	1480:1511	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	10	65	theme	interfering	1911:1921	arg1	glycans					1932:1938	interfering O-linked glycans	1911:1938	interfering O-linked glycans	1911:1938	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	2	66	theme	varicella	297:305	arg1	protein					357:363	the most abundantly expressed protein	327:363	the most abundantly expressed protein of VZV	327:370	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	66	theme	varicella	297:305	arg1	VZV					321:323	VZV	321:323	VZV	321:323	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	66	theme	varicella	297:305	arg1	virus					314:318	varicella zoster virus	297:318	varicella zoster virus (VZV)	297:324	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	6	67	theme	recombinant	1033:1043	arg1	gE					1045:1046	the recombinant gE	1029:1046	the recombinant gE of Shingrix®	1029:1059	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	0	68	theme	Immunological	128:140	arg1	Signatures					142:151	Discrete Immunological Signatures	119:151	Discrete Immunological Signatures	119:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	3	69	theme	B	517:517	arg1	response					524:531	antibody B cell response	508:531	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	5	70	link	O-linked	899:906	arg1	glycans					908:914	numerous clustered O-linked glycans	880:914	numerous clustered O-linked glycans	880:914	We previously found gE to be heavily glycosylated, not least by numerous clustered O-linked glycans, when it was produced in human fibroblasts.					
30813247	11	71	theme	Shingrix®	2079:2087	arg1	efficacy					2067:2074	the higher protective efficacy	2045:2074	the higher protective efficacy of Shingrix®	2045:2087	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	10	72	theme	peptide	1733:1739	arg1	library					1741:1747	a synthetic peptide library	1721:1747	a synthetic peptide library against serum samples from VZV-seropositive individuals	1721:1803	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	10	73	link	O-linked	1923:1930	arg1	glycans					1932:1938	interfering O-linked glycans	1911:1938	interfering O-linked glycans	1911:1938	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	8	74	theme	recombinant	1293:1303	arg1	gE					1305:1306	recombinant gE	1293:1306	recombinant gE	1293:1306	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	10	75	theme	VZV-seropositive	1776:1791	arg1	individuals					1793:1803	VZV-seropositive individuals	1776:1803	VZV-seropositive individuals	1776:1803	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	9	76	theme	O-linked	1543:1550	arg1	glycans					1552:1558	O-linked glycans	1543:1558	O-linked glycans than gE from human fibroblasts ("Zostavax situation")	1543:1612	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	10	77	theme	O-linked	1963:1970	arg1	glycans					1972:1978	specific O-linked glycans	1954:1978	specific O-linked glycans enhancing antibody binding	1954:2005	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	5	78	theme	human	941:945	arg1	fibroblasts					947:957	human fibroblasts	941:957	human fibroblasts	941:957	We previously found gE to be heavily glycosylated, not least by numerous clustered O-linked glycans, when it was produced in human fibroblasts.					
30813247	3	79	theme	used	566:569	arg1	Zostavax®					596:604	Zostavax®	596:604	Zostavax®	596:604	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	3	79	theme	used	566:569	arg1	vaccine					587:593	the currently used live attenuated vaccine	552:593	the currently used live attenuated vaccine (Zostavax®)	552:605	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	6	80	theme	hamster	1085:1091	arg1	cells					1105:1109	Chinese hamster ovary (CHO) cells	1077:1109	Chinese hamster ovary (CHO) cells	1077:1109	However, in contrast to Zostavax®, which is produced in fibroblasts, the recombinant gE of Shingrix® is expressed in Chinese hamster ovary (CHO) cells.					
30813247	7	81	theme	vaccine	1208:1214	arg1	types					1216:1220	the two vaccine types	1200:1220	the two vaccine types	1200:1220	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	1	82	theme	subunit	168:174	arg1	Shingrix®					185:193	Shingrix®	185:193	Shingrix®	185:193	A recombinant subunit vaccine (Shingrix®) was recently licensed for use against herpes zoster.					
30813247	1	82	theme	subunit	168:174	arg1	vaccine					176:182	A recombinant subunit vaccine	154:182	A recombinant subunit vaccine (Shingrix®)	154:194	A recombinant subunit vaccine (Shingrix®) was recently licensed for use against herpes zoster.					
30813247	0	83	theme	Discrete	119:126	arg1	Signatures					142:151	Discrete Immunological Signatures	119:151	Discrete Immunological Signatures	119:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	11	84	theme	other	2158:2162	arg1	viruses					2174:2180	other enveloped viruses	2158:2180	other enveloped viruses	2158:2180	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	0	85	theme	Cell	100:103	arg1	Epitopes					105:112	B Cell Epitopes	98:112	B Cell Epitopes into Discrete Immunological Signatures	98:151	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	11	86	theme	vaccines	2146:2153	arg1	development					2123:2133	development	2123:2133	development of subunit vaccines to other enveloped viruses	2123:2180	These findings may, in part, explain the higher protective efficacy of Shingrix®, and can also be of relevance for development of subunit vaccines to other enveloped viruses.					
30813247	2	87	link	O-linked	400:407	arg1	glycosylation					409:421	O-linked glycosylation	400:421	O-linked glycosylation	400:421	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	10	88	theme	O-linked	1824:1831	arg1	signature					1840:1848	the O-linked glycan signature	1820:1848	the O-linked glycan signature	1820:1848	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	5	89	gly	glycosylated	853:864	arg1	gE					836:837	gE	836:837	gE	836:837	We previously found gE to be heavily glycosylated, not least by numerous clustered O-linked glycans, when it was produced in human fibroblasts.					
30813247	8	90	theme	gE	1366:1367	arg1	pattern					1385:1391	the recombinant gE O-glycosylation pattern	1350:1391	the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®	1350:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	4	91	theme	active	695:700	arg1	infection					708:716	an active virus infection	692:716	an active virus infection	692:716	This situation is at variance with the current notion since a live vaccine, causing an active virus infection, should be far more efficient than a subunit vaccine based on only one single viral glycoprotein.					
30813247	0	92	theme	Glycoprotein	12:23	arg1	E					25:25	Recombinant Glycoprotein E	0:25	Recombinant Glycoprotein E of Varicella Zoster Virus	0:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	10	93	from	individuals	1793:1803	arg1	samples					1763:1769	serum samples	1757:1769	serum samples from VZV-seropositive individuals	1757:1803	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	8	94	theme	pattern	1385:1391	arg1	features					1338:1345	possible features	1329:1345	possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®	1329:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	7	95	theme	gE	1165:1166	arg1	occupancy					1130:1138	glycan occupancy	1123:1138	glycan occupancy	1123:1138	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	7	95	theme	gE	1165:1166	arg1	structures					1151:1160	glycan structures	1144:1160	glycan structures	1144:1160	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	3	96	theme	subunit	428:434	arg1	vaccine					436:442	The subunit vaccine	424:442	The subunit vaccine	424:442	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	0	97	theme	Zoster	40:45	arg1	Virus					47:51	Varicella Zoster Virus	30:51	Varicella Zoster Virus	30:51	Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.					
30813247	8	98	theme	vaccine	1413:1419	arg1	efficacy					1421:1428	the vaccine efficacy	1409:1428	the vaccine efficacy of Shingrix®	1409:1441	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30813247	3	99	theme	attenuated	576:585	arg1	Zostavax®					596:604	Zostavax®	596:604	Zostavax®	596:604	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	3	99	theme	attenuated	576:585	arg1	vaccine					587:593	the currently used live attenuated vaccine	552:593	the currently used live attenuated vaccine (Zostavax®)	552:605	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	2	100	theme	VZV	368:370	arg1	protein					357:363	the most abundantly expressed protein	327:363	the most abundantly expressed protein of VZV	327:370	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	100	theme	VZV	368:370	arg1	virus					314:318	varicella zoster virus	297:318	varicella zoster virus (VZV)	297:324	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	10	101	link	O-linked	1824:1831	arg1	signature					1840:1848	the O-linked glycan signature	1820:1848	the O-linked glycan signature	1820:1848	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	9	102	theme	site	1638:1641	arg1	occupancy					1643:1651	glycan site occupancy	1631:1651	glycan site occupancy	1631:1651	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	10	103	theme	B	1692:1692	arg1	epitopes					1699:1706	immunodominant B cell epitopes	1677:1706	immunodominant B cell epitopes of gE	1677:1712	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	1	104	theme	recombinant	156:166	arg1	Shingrix®					185:193	Shingrix®	185:193	Shingrix®	185:193	A recombinant subunit vaccine (Shingrix®) was recently licensed for use against herpes zoster.					
30813247	1	104	theme	recombinant	156:166	arg1	vaccine					176:182	A recombinant subunit vaccine	154:182	A recombinant subunit vaccine (Shingrix®)	154:194	A recombinant subunit vaccine (Shingrix®) was recently licensed for use against herpes zoster.					
30813247	9	105	theme	situation	1602:1610	arg1	"					1611:1611	Zostavax situation"	1593:1611	"Zostavax situation"	1592:1611	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	3	106	theme	virus-specific	461:474	arg1	cell					483:486	virus-specific CD4+ T cell	461:486	stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE	452:537	The subunit vaccine elicits stronger virus-specific CD4+ T cell response as well as antibody B cell response to gE, compared to the currently used live attenuated vaccine (Zostavax®).					
30813247	7	107	gly	occupancy	1130:1138	arg2	gE					1165:1166	gE	1165:1166	gE	1165:1166	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	9	108	from	fibroblasts	1579:1589	arg1	gE					1565:1566	gE	1565:1566	gE from human fibroblasts ("Zostavax situation")	1565:1612	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	9	109	theme	recombinant	1453:1463	arg1	gE					1465:1466	recombinant gE	1453:1466	recombinant gE produced in CHO cells ("Shingrix situation")	1453:1511	Firstly, recombinant gE produced in CHO cells ("Shingrix situation") is more scarcely decorated by O-linked glycans than gE from human fibroblasts ("Zostavax situation"), with respect to glycan site occupancy.					
30813247	7	110	theme	glycan	1123:1128	arg1	occupancy					1130:1138	glycan occupancy	1123:1138	glycan occupancy	1123:1138	Hence, the glycan occupancy and glycan structures of gE may differ considerably between the two vaccine types.					
30813247	10	111	theme	IgG	1870:1872	arg1	antibodies					1874:1883	IgG antibodies	1870:1883	IgG antibodies	1870:1883	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	2	112	theme	zoster	307:312	arg1	protein					357:363	the most abundantly expressed protein	327:363	the most abundantly expressed protein of VZV	327:370	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	112	theme	zoster	307:312	arg1	VZV					321:323	VZV	321:323	VZV	321:323	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	2	112	theme	zoster	307:312	arg1	virus					314:318	varicella zoster virus	297:318	varicella zoster virus (VZV)	297:324	This vaccine is based on glycoprotein E (gE) of varicella zoster virus (VZV), the most abundantly expressed protein of VZV, harboring sites for N- and O-linked glycosylation.					
30813247	1	113	theme	herpes	234:239	arg1	zoster					241:246	herpes zoster	234:246	herpes zoster	234:246	A recombinant subunit vaccine (Shingrix®) was recently licensed for use against herpes zoster.					
30813247	10	114	theme	gE	1711:1712	arg1	epitopes					1699:1706	immunodominant B cell epitopes	1677:1706	immunodominant B cell epitopes of gE	1677:1712	Secondly, screening of immunodominant B cell epitopes of gE, using a synthetic peptide library against serum samples from VZV-seropositive individuals, revealed that the O-linked glycan signature promoted binding of IgG antibodies via a decreased number of interfering O-linked glycans, but also via specific O-linked glycans enhancing antibody binding.					
30813247	8	115	theme	glycan	1258:1263	arg1	structures					1265:1274	the glycan structures	1254:1274	the glycan structures	1254:1274	Here, we aimed at (i) defining the glycan structures and positions of recombinant gE and (ii) identifying possible features of the recombinant gE O-glycosylation pattern contributing to the vaccine efficacy of Shingrix®.					
30668249	8	0	theme	excellent	1186:1194	arg1	robustness					1196:1205	excellent robustness	1186:1205	excellent robustness as well as retention time and peak area stability	1186:1255	The whole workflow features excellent robustness as well as retention time and peak area stability.					
30668249	5	1	theme	short	914:918	arg1	run					936:938	a short 15-min gradient run	912:938	a short 15-min gradient run	912:938	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	7	2	theme	mAb	1111:1113	arg1	content					1115:1121	mAb content	1111:1121	mAb content	1111:1121	Simultaneously, absolute quantification of mAb content in fermentation broth is achieved.					
30668249	10	3	theme	Several	1461:1467	arg1	attributes					1479:1488	Several molecular attributes	1461:1488	Several molecular attributes of the expressed therapeutic protein	1461:1525	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	3	4	theme	process	579:585	arg1	course					552:557	the course	548:557	the course of the fermentation process	548:585	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	6	5	theme	N-glycosylation	963:977	arg1	variants					994:1001	N-glycosylation and truncation variants	963:1001	N-glycosylation and truncation variants of the expressed mAb	963:1022	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	1	6	theme	therapeutic	201:211	arg1	agents					213:218	highly specific and efficient therapeutic agents	171:218	highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases	171:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	6	theme	therapeutic	201:211	arg1	antibodies					131:140	Monoclonal antibodies	120:140	Monoclonal antibodies (mAbs)	120:147	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	11	7	from	workflow	1630:1637	arg1	environment					1656:1666	an industrial environment	1642:1666	an industrial environment	1642:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	8	8	theme	peak	1237:1240	arg1	stability					1247:1255	peak area stability	1237:1255	peak area stability	1237:1255	The whole workflow features excellent robustness as well as retention time and peak area stability.					
30668249	4	9	theme	robust	708:713	arg1	workflow					715:722	a fast and robust workflow	697:722	a fast and robust workflow for the characterization of mAb variants in fermentation broth	697:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	9	10	theme	enzymatic	1269:1277	arg1	removal					1279:1285	Additional enzymatic removal	1258:1285	Additional enzymatic removal of N-glycans	1258:1298	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	5	11	theme	15-min	920:925	arg1	run					936:938	a short 15-min gradient run	912:938	a short 15-min gradient run	912:938	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	5	12	from	dilution	879:886	arg1	run					936:938	a short 15-min gradient run	912:938	a short 15-min gradient run	912:938	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	0	13	theme	fermentation	73:84	arg1	broth					86:90	fermentation broth	73:90	fermentation broth	73:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	3	14	theme	process	615:621	arg1	parameters					623:632	process parameters	615:632	process parameters	615:632	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	11	15	from	Application	1601:1611	arg1	environment					1656:1666	an industrial environment	1642:1666	an industrial environment	1642:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	6	16	theme	expressed	1010:1018	arg1	mAb					1020:1022	the expressed mAb	1006:1022	the expressed mAb	1006:1022	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	2	17	theme	drugs	501:505	arg1	efficacy					485:492	efficacy	485:492	efficacy	485:492	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	2	17	theme	drugs	501:505	arg1	safety					474:479	safety	474:479	safety	474:479	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	10	18	theme	therapeutic	1507:1517	arg1	protein					1519:1525	the expressed therapeutic protein	1493:1525	the expressed therapeutic protein	1493:1525	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	4	19	theme	fast	699:702	arg1	workflow					715:722	a fast and robust workflow	697:722	a fast and robust workflow for the characterization of mAb variants in fermentation broth	697:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	4	20	from	broth	781:785	arg1	characterization					732:747	the characterization	728:747	the characterization of mAb variants in fermentation broth	728:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	0	21	theme	method	95:100	arg1	study					113:117	a method capability study	93:117	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	9	22	theme	glycation	1329:1337	arg1	levels					1339:1344	mAb glycation levels	1325:1344	mAb glycation levels	1325:1344	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	6	23	theme	truncation	983:992	arg1	variants					994:1001	N-glycosylation and truncation variants	963:1001	N-glycosylation and truncation variants of the expressed mAb	963:1022	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	0	24	from	variants	61:68	arg1	broth					86:90	fermentation broth	73:90	fermentation broth	73:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	0	25	theme	Dilute-and-shoot	0:15	arg1	analysis					17:24	Dilute-and-shoot analysis	0:24	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	4	26	from	characterization	732:747	arg1	broth					781:785	fermentation broth	768:785	fermentation broth	768:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	7	27	from	quantification	1093:1106	arg1	broth					1139:1143	fermentation broth	1126:1143	fermentation broth	1126:1143	Simultaneously, absolute quantification of mAb content in fermentation broth is achieved.					
30668249	4	28	theme	mAb	752:754	arg1	variants					756:763	mAb variants	752:763	mAb variants in fermentation broth	752:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	6	29	theme	intact	1046:1051	arg1	level					1061:1065	the intact protein level	1042:1065	the intact protein level	1042:1065	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	3	30	theme	culture	659:665	arg1	conditions					667:676	optimal cell culture conditions	646:676	optimal cell culture conditions	646:676	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	0	31	theme	monoclonal	41:50	arg1	variants					61:68	therapeutic monoclonal antibody variants	29:68	therapeutic monoclonal antibody variants in fermentation broth	29:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	9	32	theme	relative	1384:1391	arg1	quantification					1405:1418	relative N-glycoform quantification	1384:1418	relative N-glycoform quantification	1384:1418	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	5	33	theme	Sample	788:793	arg1	preparation					795:805	Sample preparation	788:805	Sample preparation	788:805	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	3	34	theme	optimal	646:652	arg1	conditions					667:676	optimal cell culture conditions	646:676	optimal cell culture conditions	646:676	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	0	35	theme	variants	61:68	arg1	analysis					17:24	Dilute-and-shoot analysis	0:24	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	10	36	theme	desired	1576:1582	arg1	profile					1592:1598	the desired product profile	1572:1598	the desired product profile	1572:1598	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	1	37	theme	various	224:230	arg1	conditions					240:249	various medical conditions	224:249	various medical conditions	224:249	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	37	theme	various	224:230	arg1	cancer					262:267	cancer	262:267	cancer	262:267	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	37	theme	various	224:230	arg1	diseases					298:305	autoimmune diseases	287:305	autoimmune diseases	287:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	37	theme	various	224:230	arg1	inflammatory					270:281	inflammatory	270:281	inflammatory	270:281	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	2	38	theme	molecular	386:394	arg1	variants					396:403	molecular variants	386:403	molecular variants	386:403	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	9	39	theme	isobaric	1435:1442	arg1	galactosylation					1444:1458	isobaric galactosylation	1435:1458	isobaric galactosylation	1435:1458	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	11	40	from	environment	1656:1666	arg1	Application					1601:1611	Application	1601:1611	Application of the described workflow in an industrial environment	1601:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	7	41	theme	fermentation	1126:1137	arg1	broth					1139:1143	fermentation broth	1126:1143	fermentation broth	1126:1143	Simultaneously, absolute quantification of mAb content in fermentation broth is achieved.					
30668249	11	42	theme	mAb	1726:1728	arg1	production					1730:1739	mAb production	1726:1739	mAb production	1726:1739	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	7	43	theme	content	1115:1121	arg1	quantification					1093:1106	absolute quantification	1084:1106	absolute quantification of mAb content in fermentation broth	1084:1143	Simultaneously, absolute quantification of mAb content in fermentation broth is achieved.					
30668249	3	44	from	monitoring	518:527	arg1	course					552:557	the course	548:557	the course of the fermentation process	548:585	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	8	45	theme	whole	1162:1166	arg1	workflow					1168:1175	The whole workflow	1158:1175	The whole workflow	1158:1175	The whole workflow features excellent robustness as well as retention time and peak area stability.					
30668249	11	46	theme	targeted	1753:1760	arg1	development					1773:1783	targeted biosimilar development	1753:1783	targeted biosimilar development	1753:1783	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	3	47	theme	variants	536:543	arg1	monitoring					518:527	monitoring	518:527	monitoring of mAb variants in the course of the fermentation process	518:585	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	8	48	theme	area	1242:1245	arg1	stability					1247:1255	peak area stability	1237:1255	peak area stability	1237:1255	The whole workflow features excellent robustness as well as retention time and peak area stability.					
30668249	7	49	theme	absolute	1084:1091	arg1	quantification					1093:1106	absolute quantification	1084:1106	absolute quantification of mAb content in fermentation broth	1084:1143	Simultaneously, absolute quantification of mAb content in fermentation broth is achieved.					
30668249	10	50	theme	molecular	1469:1477	arg1	attributes					1479:1488	Several molecular attributes	1461:1488	Several molecular attributes of the expressed therapeutic protein	1461:1525	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	3	51	theme	fermentation	566:577	arg1	process					579:585	the fermentation process	562:585	the fermentation process	562:585	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	1	52	theme	specific	178:185	arg1	agents					213:218	highly specific and efficient therapeutic agents	171:218	highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases	171:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	52	theme	specific	178:185	arg1	antibodies					131:140	Monoclonal antibodies	120:140	Monoclonal antibodies (mAbs)	120:147	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	11	53	theme	described	1620:1628	arg1	workflow					1630:1637	the described workflow	1616:1637	the described workflow in an industrial environment	1616:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	11	54	theme	workflow	1630:1637	arg1	Application					1601:1611	Application	1601:1611	Application of the described workflow in an industrial environment	1601:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	2	55	theme	protein	311:317	arg1	production					319:328	protein production	311:328	protein production in cellular systems	311:348	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	1	56	theme	efficient	191:199	arg1	agents					213:218	highly specific and efficient therapeutic agents	171:218	highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases	171:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	56	theme	efficient	191:199	arg1	antibodies					131:140	Monoclonal antibodies	120:140	Monoclonal antibodies (mAbs)	120:147	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	3	57	theme	instant	597:603	arg1	tuning					605:610	instant tuning	597:610	instant tuning of process parameters	597:632	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	8	58	theme	retention	1218:1226	arg1	time					1228:1231	retention time	1218:1231	retention time	1218:1231	The whole workflow features excellent robustness as well as retention time and peak area stability.					
30668249	6	59	theme	single	946:951	arg1	analysis					953:960	a single analysis	944:960	a single analysis	944:960	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	0	60	from	broth	86:90	arg1	analysis					17:24	Dilute-and-shoot analysis	0:24	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	9	61	theme	Additional	1258:1267	arg1	removal					1279:1285	Additional enzymatic removal	1258:1285	Additional enzymatic removal of N-glycans	1258:1298	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	1	62	theme	Monoclonal	120:129	arg1	mAbs					143:146	mAbs	143:146	mAbs	143:146	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	62	theme	Monoclonal	120:129	arg1	agents					213:218	highly specific and efficient therapeutic agents	171:218	highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases	171:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	62	theme	Monoclonal	120:129	arg1	antibodies					131:140	Monoclonal antibodies	120:140	Monoclonal antibodies (mAbs)	120:147	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	3	63	theme	parameters	623:632	arg1	tuning					605:610	instant tuning	597:610	instant tuning of process parameters	597:632	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	5	64	theme	gradient	927:934	arg1	run					936:938	a short 15-min gradient run	912:938	a short 15-min gradient run	912:938	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	9	65	theme	N-glycans	1290:1298	arg1	removal					1279:1285	Additional enzymatic removal	1258:1285	Additional enzymatic removal of N-glycans	1258:1298	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	5	66	theme	fermentation	830:841	arg1	broth					843:847	the fermentation broth	826:847	the fermentation broth	826:847	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	5	67	theme	HPLC-MS	892:898	arg1	analysis					900:907	HPLC-MS analysis	892:907	HPLC-MS analysis	892:907	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	6	68	theme	mAb	1020:1022	arg1	variants					994:1001	N-glycosylation and truncation variants	963:1001	N-glycosylation and truncation variants of the expressed mAb	963:1022	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	0	69	from	analysis	17:24	arg1	broth					86:90	fermentation broth	73:90	fermentation broth	73:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	10	70	theme	expressed	1497:1505	arg1	protein					1519:1525	the expressed therapeutic protein	1493:1525	the expressed therapeutic protein	1493:1525	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	0	71	theme	capability	102:111	arg1	study					113:117	a method capability study	93:117	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	9	72	theme	mAb	1325:1327	arg1	levels					1339:1344	mAb glycation levels	1325:1344	mAb glycation levels	1325:1344	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	2	73	from	production	319:328	arg1	systems					342:348	cellular systems	333:348	cellular systems	333:348	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	4	74	theme	variants	756:763	arg1	characterization					732:747	the characterization	728:747	the characterization of mAb variants in fermentation broth	728:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	9	75	theme	levels	1339:1344	arg1	determination					1308:1320	determination	1308:1320	determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation	1308:1458	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	4	76	from	variants	756:763	arg1	broth					781:785	fermentation broth	768:785	fermentation broth	768:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	4	77	theme	fermentation	768:779	arg1	broth					781:785	fermentation broth	768:785	fermentation broth	768:785	Here, we describe a fast and robust workflow for the characterization of mAb variants in fermentation broth.					
30668249	10	78	theme	protein	1519:1525	arg1	attributes					1479:1488	Several molecular attributes	1461:1488	Several molecular attributes of the expressed therapeutic protein	1461:1525	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	2	79	theme	variants	396:403	arg1	multitude					373:381	a multitude	371:381	a multitude of molecular variants	371:403	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	0	80	theme	therapeutic	29:39	arg1	variants					61:68	therapeutic monoclonal antibody variants	29:68	therapeutic monoclonal antibody variants in fermentation broth	29:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	1	81	theme	autoimmune	287:296	arg1	diseases					298:305	autoimmune diseases	287:305	autoimmune diseases	287:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	5	82	from	analysis	900:907	arg1	run					936:938	a short 15-min gradient run	912:938	a short 15-min gradient run	912:938	Sample preparation is minimal in that the fermentation broth is shortly centrifuged before dilution and HPLC-MS analysis in a short 15-min gradient run.					
30668249	6	83	theme	protein	1053:1059	arg1	level					1061:1065	the intact protein level	1042:1065	the intact protein level	1042:1065	In a single analysis, N-glycosylation and truncation variants of the expressed mAb are identified at the intact protein level.					
30668249	3	84	theme	cell	654:657	arg1	conditions					667:676	optimal cell culture conditions	646:676	optimal cell culture conditions	646:676	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30668249	2	85	theme	stringent	437:445	arg1	control					447:453	stringent control in order to ensure safety and efficacy of the drugs	437:505	stringent control in order to ensure safety and efficacy of the drugs	437:505	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	0	86	theme	antibody	52:59	arg1	variants					61:68	therapeutic monoclonal antibody variants	29:68	therapeutic monoclonal antibody variants in fermentation broth	29:90	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	11	87	theme	biosimilar	1762:1771	arg1	development					1773:1783	targeted biosimilar development	1753:1783	targeted biosimilar development	1753:1783	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	2	88	theme	mAbs	423:426	arg1	manufacturing					406:418	manufacturing	406:418	manufacturing of mAbs	406:426	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	9	89	theme	N-glycoform	1393:1403	arg1	quantification					1405:1418	relative N-glycoform quantification	1384:1418	relative N-glycoform quantification	1384:1418	Additional enzymatic removal of N-glycans enables determination of mAb glycation levels, which are subsequently considered in relative N-glycoform quantification to correct for isobaric galactosylation.					
30668249	10	90	theme	product	1584:1590	arg1	profile					1592:1598	the desired product profile	1572:1598	the desired product profile	1572:1598	Several molecular attributes of the expressed therapeutic protein may thus be continuously monitored to ensure the desired product profile.					
30668249	11	91	theme	industrial	1645:1654	arg1	environment					1656:1666	an industrial environment	1642:1666	an industrial environment	1642:1666	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	11	92	theme	in-process	1704:1713	arg1	control					1715:1721	in-process control	1704:1721	in-process control	1704:1721	Application of the described workflow in an industrial environment may therefore substantially enhance in-process control in mAb production, as well as targeted biosimilar development.					
30668249	2	93	theme	cellular	333:340	arg1	systems					342:348	cellular systems	333:348	cellular systems	333:348	As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs requires stringent control in order to ensure safety and efficacy of the drugs.					
30668249	1	94	theme	medical	232:238	arg1	conditions					240:249	various medical conditions	224:249	various medical conditions	224:249	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	94	theme	medical	232:238	arg1	cancer					262:267	cancer	262:267	cancer	262:267	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	94	theme	medical	232:238	arg1	diseases					298:305	autoimmune diseases	287:305	autoimmune diseases	287:305	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	1	94	theme	medical	232:238	arg1	inflammatory					270:281	inflammatory	270:281	inflammatory	270:281	Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer, inflammatory and autoimmune diseases.					
30668249	0	95	dep	analysis	17:24	arg1	study					113:117	a method capability study	93:117	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.	0:118	Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study.					
30668249	3	96	theme	mAb	532:534	arg1	variants					536:543	mAb variants	532:543	mAb variants	532:543	Moreover, monitoring of mAb variants in the course of the fermentation process may allow instant tuning of process parameters to maintain optimal cell culture conditions.					
30827619	6	0	theme	A.	949:950	arg1	β-galactosidase					959:973	wild-type A. oryzae β-galactosidase	939:973	wild-type A. oryzae β-galactosidase	939:973	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	1	1	theme	prebiotic	205:213	arg1	effects					215:221	beneficial prebiotic effects	194:221	beneficial prebiotic effects	194:221	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30827619	0	2	theme	galactooligosaccharide	77:98	arg1	production					100:109	galactooligosaccharide production	77:109	galactooligosaccharide production	77:109	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	0	3	from	design	9:14	arg1	oryzae					59:64	Aspergillus oryzae	47:64	Aspergillus oryzae	47:64	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	6	4	theme	reaction	844:851	arg1	mechanism					853:861	the β-galactosidase reaction mechanism	824:861	the β-galactosidase reaction mechanism	824:861	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	5	5	theme	galactooligosaccharide	671:692	arg1	highest					722:728	highest	722:728	highest	722:728	The galactooligosaccharide yield of N140C/W806F is the highest reported to date.					
30827619	5	5	theme	galactooligosaccharide	671:692	arg1	yield					694:698	The galactooligosaccharide yield	667:698	The galactooligosaccharide yield of N140C/W806F	667:713	The galactooligosaccharide yield of N140C/W806F is the highest reported to date.					
30827619	6	6	theme	β-galactosidase	828:842	arg1	mechanism					853:861	the β-galactosidase reaction mechanism	824:861	the β-galactosidase reaction mechanism	824:861	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	6	7	from	useful	900:905	arg1	applications					921:932	industrial applications	910:932	industrial applications	910:932	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	0	8	from	oryzae	59:64	arg1	design					9:14	Rational design	0:14	Rational design of the beta-galactosidase from Aspergillus oryzae	0:64	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	0	8	from	oryzae	59:64	arg1	beta-galactosidase					23:40	the beta-galactosidase	19:40	the beta-galactosidase from Aspergillus oryzae	19:64	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	3	9	used	used	414:417	arg2	mutagenesis					397:407	site-directed mutagenesis	383:407	site-directed mutagenesis	383:407	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	9	used	used	414:417	arg2	design					372:377	Rational design	363:377	Rational design	363:377	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	5	10	theme	N140C/W806F	703:713	arg1	highest					722:728	highest	722:728	highest	722:728	The galactooligosaccharide yield of N140C/W806F is the highest reported to date.					
30827619	5	10	theme	N140C/W806F	703:713	arg1	yield					694:698	The galactooligosaccharide yield	667:698	The galactooligosaccharide yield of N140C/W806F	667:713	The galactooligosaccharide yield of N140C/W806F is the highest reported to date.					
30827619	4	11	theme	substantial	606:616	arg1	improvements					618:629	substantial improvements	606:629	substantial improvements	606:629	The galactooligosaccharide yields of N140C (50.7%), W806F (49.3%), and N140C/W806F (59.8%) represent substantial improvements over that of the wild-type (35.7%).					
30827619	6	12	from	applications	921:932	arg1	useful					900:905	useful	900:905	useful	900:905	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	6	13	theme	industrial	910:919	arg1	applications					921:932	industrial applications	910:932	industrial applications	910:932	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	2	14	theme	industrial	338:347	arg1	applications					349:360	industrial applications	338:360	industrial applications	338:360	However, its low galactooligosaccharide yield greatly limits its use in industrial applications.					
30827619	6	15	dep	A.	949:950	arg1	oryzae					952:957	A. oryzae	949:957	wild-type A. oryzae β-galactosidase	939:973	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	1	16	with	galactooligosaccharides	165:187	arg1	effects					215:221	beneficial prebiotic effects	194:221	beneficial prebiotic effects	194:221	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30827619	6	17	theme	further	807:813	arg1	study					815:819	further study	807:819	further study of the β-galactosidase reaction mechanism	807:861	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	0	18	theme	Rational	0:7	arg1	design					9:14	Rational design	0:14	Rational design of the beta-galactosidase from Aspergillus oryzae	0:64	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	3	19	theme	A.	447:448	arg1	W806F					481:485	W806F	481:485	W806F	481:485	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	19	theme	A.	447:448	arg1	mutants					436:442	three mutants	430:442	three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F	430:502	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	19	theme	A.	447:448	arg1	N140C/W806F					492:502	N140C/W806F	492:502	N140C/W806F	492:502	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	19	theme	A.	447:448	arg1	N140C					474:478	N140C	474:478	N140C	474:478	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	1	20	from	oryzae	149:154	arg1	β-galactosidase					116:130	The β-galactosidase	112:130	The β-galactosidase from Aspergillus oryzae	112:154	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30827619	1	21	theme	lactose	257:263	arg1	transglycosylation					235:252	the transglycosylation	231:252	the transglycosylation of lactose	231:263	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30827619	2	22	from	use	331:333	arg1	applications					349:360	industrial applications	338:360	industrial applications	338:360	However, its low galactooligosaccharide yield greatly limits its use in industrial applications.					
30827619	0	23	theme	beta-galactosidase	23:40	arg1	design					9:14	Rational design	0:14	Rational design of the beta-galactosidase from Aspergillus oryzae	0:64	Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production.					
30827619	2	24	theme	galactooligosaccharide	283:304	arg1	yield					306:310	its low galactooligosaccharide yield	275:310	its low galactooligosaccharide yield	275:310	However, its low galactooligosaccharide yield greatly limits its use in industrial applications.					
30827619	6	25	theme	novel	786:790	arg1	strategies					792:801	novel strategies	786:801	novel strategies for further study of the β-galactosidase reaction mechanism	786:861	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	6	26	dep	mutants	880:886	arg1	useful					900:905	useful	900:905	useful	900:905	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	2	27	theme	low	279:281	arg1	yield					306:310	its low galactooligosaccharide yield	275:310	its low galactooligosaccharide yield	275:310	However, its low galactooligosaccharide yield greatly limits its use in industrial applications.					
30827619	3	28	dep	mutants	436:442	arg1	mutants					436:442	three mutants	430:442	three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F	430:502	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	28	dep	mutants	436:442	arg1	W806F					481:485	W806F	481:485	W806F	481:485	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	28	dep	mutants	436:442	arg1	N140C/W806F					492:502	N140C/W806F	492:502	N140C/W806F	492:502	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	28	dep	mutants	436:442	arg1	N140C					474:478	N140C	474:478	N140C	474:478	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	3	29	theme	site-directed	383:395	arg1	mutagenesis					397:407	site-directed mutagenesis	383:407	site-directed mutagenesis	383:407	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	6	30	theme	wild-type	939:947	arg1	β-galactosidase					959:973	wild-type A. oryzae β-galactosidase	939:973	wild-type A. oryzae β-galactosidase	939:973	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	3	31	theme	Rational	363:370	arg1	design					372:377	Rational design	363:377	Rational design	363:377	Rational design and site-directed mutagenesis were used to produce three mutants of A. oryzae β-galactosidase: N140C, W806F, and N140C/W806F.					
30827619	6	32	theme	design	761:766	arg1	approach					768:775	Our rational design approach	748:775	Our rational design approach	748:775	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	1	33	gly	transglycosylation	235:252	arg1	lactose					257:263	lactose	257:263	lactose	257:263	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30827619	6	34	theme	rational	752:759	arg1	approach					768:775	Our rational design approach	748:775	Our rational design approach	748:775	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	6	35	theme	mechanism	853:861	arg1	study					815:819	further study	807:819	further study of the β-galactosidase reaction mechanism	807:861	Our rational design approach suggests novel strategies for further study of the β-galactosidase reaction mechanism and has produced mutants may be more useful in industrial applications than wild-type A. oryzae β-galactosidase.					
30827619	1	36	theme	beneficial	194:203	arg1	effects					215:221	beneficial prebiotic effects	194:221	beneficial prebiotic effects	194:221	The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with beneficial prebiotic effects through the transglycosylation of lactose.					
30275012	0	0	theme	complex	73:79	arg1	glycan					81:86	a complex glycan	71:86	a complex glycan required for normal cell growth and morphology	71:133	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	3	1	gly	glycosylated	397:408	arg1	species					373:379	many bacterial and archaeal species	345:379	many bacterial and archaeal species	345:379	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	3	1	gly	glycosylated	397:408	arg1	S-layer					386:392	the S-layer	382:392	the S-layer	382:392	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	3	1	gly	glycosylated	397:408	arg2	S-layer					386:392	the S-layer	382:392	the S-layer	382:392	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	4	2	theme	subunit	647:653	arg1	subunit					647:653	the S-layer low-molecular-weight subunit	614:653	the S-layer low-molecular-weight subunit	614:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	2	theme	subunit	647:653	arg1	Thr-38					604:609	Thr-38	604:609	Thr-38	604:609	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	8	3	theme	cell	1624:1627	arg1	shape					1629:1633	cell shape	1624:1633	cell shape	1624:1633	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	7	4	contain	containing	1133:1142	arg2	genes					1144:1148	genes	1144:1148	genes for synthesis and protein attachment of this glycan	1144:1200	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	7	4	contain	containing	1133:1142	arg1	locus					1127:1131	a 24-kb locus	1119:1131	a 24-kb locus containing genes for synthesis and protein attachment of this glycan	1119:1200	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	4	5	theme	low-molecular-weight	626:645	arg1	subunit					647:653	the S-layer low-molecular-weight subunit	614:653	the S-layer low-molecular-weight subunit	614:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	6	theme	type	538:541	arg1	Ox247					547:551	Ox247	547:551	Ox247	547:551	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	6	theme	type	538:541	arg1	strain					511:516	a C. difficile strain	496:516	a C. difficile strain of S-layer cassette type 11	496:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	3	7	theme	many	345:348	arg1	species					373:379	many bacterial and archaeal species	345:379	many bacterial and archaeal species	345:379	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	6	8	theme	sequence	819:826	arg1	-4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide					828:867	the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide	815:867	the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide	815:867	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	8	9	dep	sporulation	1371:1381	arg1	indicate					1460:1467	indicate	1460:1467	indicate that the S-layer protein of SLCT-11 strains displays a complex glycan	1460:1537	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	9	dep	sporulation	1371:1381	arg1	suggest					1543:1549	suggest	1543:1549	suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer	1543:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	10	theme	S-layer	1339:1345	arg1	modification					1347:1358	this S-layer modification	1334:1358	this S-layer modification	1334:1358	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	11	theme	difficile	1587:1595	arg1	sporulation					1597:1607	C. difficile sporulation	1584:1607	C. difficile sporulation	1584:1607	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	11	theme	difficile	1587:1595	arg1	discovery					1638:1646	a discovery	1636:1646	a discovery with implications for the development of antimicrobials targeting the S-layer	1636:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	3	12	theme	bacterial	350:358	arg1	species					373:379	many bacterial and archaeal species	345:379	many bacterial and archaeal species	345:379	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	8	13	theme	S-layer	1478:1484	arg1	protein					1486:1492	the S-layer protein	1474:1492	the S-layer protein of SLCT-11 strains	1474:1511	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	14	with	discovery	1638:1646	arg1	implications					1653:1664	implications	1653:1664	implications for the development of antimicrobials targeting the S-layer	1653:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	15	theme	C.	1584:1585	arg1	sporulation					1597:1607	C. difficile sporulation	1584:1607	C. difficile sporulation	1584:1607	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	15	theme	C.	1584:1585	arg1	discovery					1638:1646	a discovery	1636:1646	a discovery with implications for the development of antimicrobials targeting the S-layer	1636:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	6	16	with	pentasaccharide	887:901	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	17	theme	rhamnose	1075:1082	arg1	residue					1084:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	0	18	theme	normal	101:106	arg1	growth					113:118	cell growth	108:118	cell growth	108:118	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	6	19	attach	attached	1032:1039	arg1	residue					1084:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	19	attach	attached	1032:1039	arg2	cyclophosphoryl-rhamnose					1007:1030	a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose	976:1030	(iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	970:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	20	link	peptide-linked	779:792	arg1	tetrasaccharide					794:808	a core peptide-linked tetrasaccharide	772:808	three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	753:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	21	theme	-4-β-Rha-4-α-Glc-3-β-Rha-4-	921:947	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	7	22	theme	Ox247	1097:1101	arg1	genome					1103:1108	The Ox247 genome	1093:1108	The Ox247 genome	1093:1108	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	2	23	theme	S	266:266	arg1	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	23	theme	S	266:266	arg1	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	8	24	theme	difficile	1425:1433	arg1	sporulation					1371:1381	sporulation	1371:1381	sporulation	1371:1381	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	24	theme	difficile	1425:1433	arg1	length					1389:1394	cell length	1384:1394	cell length	1384:1394	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	24	theme	difficile	1425:1433	arg1	formation					1409:1417	biofilm formation	1401:1417	biofilm formation	1401:1417	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	1	25	theme	bacterial	163:171	arg1	pathogen					173:180	a bacterial pathogen	161:180	a bacterial pathogen that causes major health challenges worldwide	161:226	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	1	25	theme	bacterial	163:171	arg1	difficile					148:156	Clostridium difficile	136:156	Clostridium difficile	136:156	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	0	26	theme	S-layer	4:10	arg1	protein					12:18	The S-layer protein	0:18	The S-layer protein of a Clostridium difficile SLCT-11 strain	0:60	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	6	27	theme	peptide-linked	779:792	arg1	tetrasaccharide					794:808	a core peptide-linked tetrasaccharide	772:808	three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	753:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	28	dep	domains	759:765	arg1	tetrasaccharide					794:808	a core peptide-linked tetrasaccharide	772:808	three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	753:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	29	theme	2,3	1003:1005	arg1	cyclophosphoryl-rhamnose					1007:1030	a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose	976:1030	(iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	970:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	30	theme	sequence	912:919	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	2	31	theme	proteinaceous	295:307	arg1	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	31	theme	proteinaceous	295:307	arg1	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	8	32	theme	cell	1384:1387	arg1	length					1389:1394	cell length	1384:1394	cell length	1384:1394	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	6	33	theme	end-terminal	990:1001	arg1	cyclophosphoryl-rhamnose					1007:1030	a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose	976:1030	(iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	970:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	7	34	theme	24-kb	1121:1125	arg1	locus					1127:1131	a 24-kb locus	1119:1131	a 24-kb locus containing genes for synthesis and protein attachment of this glycan	1119:1200	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	6	35	with	tetrasaccharide	794:808	arg1	-4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide					828:867	the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide	815:867	the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide	815:867	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	35	with	tetrasaccharide	794:808	arg1	cyclophosphoryl-rhamnose					1007:1030	a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose	976:1030	(iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	970:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	35	with	tetrasaccharide	794:808	arg1	pentasaccharide					887:901	a repeating pentasaccharide	875:901	(ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	870:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	7	36	contain	contains	1110:1117	arg2	locus					1127:1131	a 24-kb locus	1119:1131	a 24-kb locus containing genes for synthesis and protein attachment of this glycan	1119:1200	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	7	36	contain	contains	1110:1117	arg1	genome					1103:1108	The Ox247 genome	1093:1108	The Ox247 genome	1093:1108	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	2	37	theme	para-crystalline	278:293	arg1	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	37	theme	para-crystalline	278:293	arg1	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	6	38	theme	nonreducing	978:988	arg1	cyclophosphoryl-rhamnose					1007:1030	a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose	976:1030	(iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	970:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	3	39	theme	archaeal	364:371	arg1	species					373:379	many bacterial and archaeal species	345:379	many bacterial and archaeal species	345:379	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	4	40	theme	cassette	529:536	arg1	type					538:541	S-layer cassette type 11	521:544	S-layer cassette type 11	521:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	0	41	theme	cell	108:111	arg1	growth					113:118	cell growth	108:118	cell growth	108:118	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	6	42	theme	core	774:777	arg1	tetrasaccharide					794:808	a core peptide-linked tetrasaccharide	772:808	three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue	753:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	43	dep	cyclophosphoryl-rhamnose	1007:1030	arg1	iii					971:973	iii	971:973	iii	971:973	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	8	44	theme	antimicrobials	1689:1702	arg1	development					1674:1684	the development	1670:1684	the development of antimicrobials targeting the S-layer	1670:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	1	45	theme	major	194:198	arg1	worldwide					218:226	major health challenges worldwide	194:226	major health challenges worldwide	194:226	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	4	46	theme	S-layer	521:527	arg1	type					538:541	S-layer cassette type 11	521:544	S-layer cassette type 11	521:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	8	47	theme	biofilm	1401:1407	arg1	formation					1409:1417	biofilm formation	1401:1417	biofilm formation	1401:1417	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	1	48	theme	health	200:205	arg1	worldwide					218:226	major health challenges worldwide	194:226	major health challenges worldwide	194:226	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	6	49	theme	repeating	877:885	arg1	pentasaccharide					887:901	a repeating pentasaccharide	875:901	(ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	870:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	50	theme	α-Rib-3-	949:956	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	8	51	theme	C.	1422:1423	arg1	difficile					1425:1433	C. difficile	1422:1433	C. difficile	1422:1433	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	1	52	theme	challenges	207:216	arg1	worldwide					218:226	major health challenges worldwide	194:226	major health challenges worldwide	194:226	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	0	53	theme	SLCT-11	47:53	arg1	strain					55:60	a Clostridium difficile SLCT-11 strain	23:60	a Clostridium difficile SLCT-11 strain	23:60	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	8	54	theme	complex	1524:1530	arg1	glycan					1532:1537	a complex glycan	1522:1537	a complex glycan	1522:1537	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	6	55	with	cyclophosphoryl-rhamnose	1007:1030	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	56	theme	sub-terminal	1062:1073	arg1	residue					1084:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	6	57	with	-4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide	828:867	arg1	β-Rha-					958:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-	908:963	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	0	58	theme	difficile	37:45	arg1	strain					55:60	a Clostridium difficile SLCT-11 strain	23:60	a Clostridium difficile SLCT-11 strain	23:60	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	4	59	theme	difficile	501:509	arg1	Ox247					547:551	Ox247	547:551	Ox247	547:551	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	59	theme	difficile	501:509	arg1	strain					511:516	a C. difficile strain	496:516	a C. difficile strain of S-layer cassette type 11	496:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	8	60	theme	glycan	1290:1295	arg1	formation					1272:1280	formation	1272:1280	formation of this glycan	1272:1295	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	6	61	dep	pentasaccharide	887:901	arg1	ii					871:872	ii	871:872	ii	871:872	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	7	62	theme	protein	1168:1174	arg1	attachment					1176:1185	protein attachment	1168:1185	protein attachment	1168:1185	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	2	63	theme	cell	331:334	arg1	wall					336:339	the cell wall	327:339	the cell wall	327:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	4	64	theme	C.	498:499	arg1	Ox247					547:551	Ox247	547:551	Ox247	547:551	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	64	theme	C.	498:499	arg1	strain					511:516	a C. difficile strain	496:516	a C. difficile strain of S-layer cassette type 11	496:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	65	attach	attached	575:582	arg2	glycan					568:573	a complex glycan	558:573	a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit	558:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	65	attach	attached	575:582	arg1	subunit					647:653	the S-layer low-molecular-weight subunit	614:653	the S-layer low-molecular-weight subunit	614:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	65	attach	attached	575:582	arg1	Thr-38					604:609	Thr-38	604:609	Thr-38	604:609	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	8	66	from	Mutations	1203:1211	arg1	genes					1216:1220	genes	1216:1220	genes within this locus	1216:1238	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	6	67	dep	tetrasaccharide	794:808	arg1	i					769:769	i	769:769	i	769:769	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	8	68	theme	strains	1505:1511	arg1	protein					1486:1492	the S-layer protein	1474:1492	the S-layer protein of SLCT-11 strains	1474:1511	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	2	69	theme	surface	257:263	arg1	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	69	theme	surface	257:263	arg1	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	3	70	theme	C.	463:464	arg1	difficile					466:474	C. difficile	463:474	C. difficile	463:474	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	7	71	theme	glycan	1195:1200	arg1	synthesis					1154:1162	synthesis	1154:1162	synthesis	1154:1162	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	7	71	theme	glycan	1195:1200	arg1	attachment					1176:1185	protein attachment	1168:1185	protein attachment	1168:1185	The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan.					
30275012	6	72	theme	ribose-branched	1046:1060	arg1	residue					1084:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	a ribose-branched sub-terminal rhamnose residue	1044:1090	We present evidence that it is composed of three domains: (i) a core peptide-linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end-terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue.					
30275012	2	73	theme	well-characterized	238:255	arg1	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	73	theme	well-characterized	238:255	arg1	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	4	74	theme	S-layer	618:624	arg1	subunit					647:653	the S-layer low-molecular-weight subunit	614:653	the S-layer low-molecular-weight subunit	614:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	3	75	theme	such	418:421	arg1	modifications					423:435	no such modifications	415:435	no such modifications	415:435	In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile.					
30275012	4	76	theme	complex	560:566	arg1	glycan					568:573	a complex glycan	558:573	a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit	558:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	0	77	theme	strain	55:60	arg1	protein					12:18	The S-layer protein	0:18	The S-layer protein of a Clostridium difficile SLCT-11 strain	0:60	The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology.					
30275012	4	78	contain	has	554:556	arg2	glycan					568:573	a complex glycan	558:573	a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit	558:653	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	78	contain	has	554:556	arg1	Ox247					547:551	Ox247	547:551	Ox247	547:551	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	4	78	contain	has	554:556	arg1	strain					511:516	a C. difficile strain	496:516	a C. difficile strain of S-layer cassette type 11	496:544	Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit.					
30275012	1	79	theme	Clostridium	136:146	arg1	pathogen					173:180	a bacterial pathogen	161:180	a bacterial pathogen that causes major health challenges worldwide	161:226	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	1	79	theme	Clostridium	136:146	arg1	difficile					148:156	Clostridium difficile	136:156	Clostridium difficile	136:156	Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide.					
30275012	8	80	theme	shape	1629:1633	arg1	control					1613:1619	control	1613:1619	control of cell shape	1613:1633	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	80	theme	shape	1629:1633	arg1	sporulation					1597:1607	C. difficile sporulation	1584:1607	C. difficile sporulation	1584:1607	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	8	80	theme	shape	1629:1633	arg1	discovery					1638:1646	a discovery	1636:1646	a discovery with implications for the development of antimicrobials targeting the S-layer	1636:1724	Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.					
30275012	2	81	contain	has	232:234	arg1	It					229:230	It	229:230	It	229:230	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	81	contain	has	232:234	arg2	layer					309:313	a para-crystalline proteinaceous layer	276:313	a para-crystalline proteinaceous layer surrounding the cell wall	276:339	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
30275012	2	81	contain	has	232:234	arg2	-layer					268:273	a well-characterized surface (S)-layer	236:273	a well-characterized surface (S)-layer	236:273	It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall.					
31654087	9	0	theme	carbohydrate	1314:1325	arg1	functions					1327:1335	crucial carbohydrate functions	1306:1335	crucial carbohydrate functions	1306:1335	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	5	1	theme	biotinylated	651:662	arg1	lectins					664:670	biotinylated lectins	651:670	biotinylated lectins	651:670	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	4	2	theme	specific	571:578	arg1	antigens					593:600	specific carbohydrate antigens	571:600	specific carbohydrate antigens in human skin fibroblasts	571:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	0	3	theme	rosmarinic	66:75	arg1	acid					77:80	rosmarinic acid	66:80	rosmarinic acid	66:80	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	4	4	from	effect	523:528	arg1	antigens					593:600	specific carbohydrate antigens	571:600	specific carbohydrate antigens in human skin fibroblasts	571:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	6	5	theme	responsible	832:842	arg1	glycosyltransferases					811:830	selected glycosyltransferases	802:830	selected glycosyltransferases responsible for formation of sugar antigens	802:874	RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens.					
31654087	9	6	theme	specific	1262:1269	arg1	antigens					1277:1284	specific sugar antigens	1262:1284	specific sugar antigens	1262:1284	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	4	7	from	antigens	593:600	arg1	fibroblasts					616:626	human skin fibroblasts	605:626	human skin fibroblasts	605:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	8	8	theme	RA	1045:1046	arg1	doses					1036:1040	All used doses	1027:1040	All used doses of RA	1027:1046	All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA.					
31654087	6	9	theme	glycosyltransferases	811:830	arg1	mRNA					794:797	mRNA	794:797	mRNA of selected glycosyltransferases responsible for formation of sugar antigens	794:874	RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens.					
31654087	2	10	theme	signaling	302:310	arg1	pathways					312:319	signaling pathways	302:319	signaling pathways	302:319	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	7	11	gly	fucosylated	964:974	arg1	antigens					976:983	fucosylated antigens	964:983	fucosylated antigens	964:983	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	9	12	theme	skin	1340:1343	arg1	fibroblasts					1345:1355	skin fibroblasts	1340:1355	skin fibroblasts	1340:1355	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	6	13	theme	selected	802:809	arg1	glycosyltransferases					811:830	selected glycosyltransferases	802:830	selected glycosyltransferases responsible for formation of sugar antigens	802:874	RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens.					
31654087	7	14	theme	Rosmarinic	877:886	arg1	acid					888:891	Rosmarinic acid	877:891	Rosmarinic acid	877:891	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	4	15	theme	carbohydrate	580:591	arg1	antigens					593:600	specific carbohydrate antigens	571:600	specific carbohydrate antigens in human skin fibroblasts	571:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	9	16	from	functions	1327:1335	arg1	fibroblasts					1345:1355	skin fibroblasts	1340:1355	skin fibroblasts	1340:1355	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	5	17	with	test	641:644	arg1	lectins					664:670	biotinylated lectins	651:670	biotinylated lectins	651:670	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	7	18	theme	fucosylated	964:974	arg1	antigens					976:983	fucosylated antigens	964:983	fucosylated antigens	964:983	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	3	19	theme	natural	348:354	arg1	flavonoid					356:364	a natural flavonoid	346:364	a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic	346:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	19	theme	natural	348:354	arg1	acid					333:336	Rosmarinic acid	322:336	Rosmarinic acid (RA)	322:341	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	5	20	theme	ELISA-liked	629:639	arg1	test					641:644	ELISA-liked test	629:644	ELISA-liked test with biotinylated lectins	629:670	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	7	21	theme	sialylated	946:955	arg1	forms					957:961	their sialylated forms	940:961	their sialylated forms	940:961	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	7	22	gly	sialylated	946:955	arg1	forms					957:961	their sialylated forms	940:961	their sialylated forms	940:961	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	9	23	theme	cell	1376:1379	arg1	adhesion					1381:1388	cell adhesion	1376:1388	cell adhesion	1376:1388	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	7	24	theme	forms	957:961	arg1	expression					907:916	the expression	903:916	the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens	903:1024	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	8	25	theme	core	1072:1075	arg1	mRNA					1105:1108	core 1 β1-3galactosyltransferase mRNA	1072:1108	core 1 β1-3galactosyltransferase mRNA	1072:1108	All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA.					
31654087	6	26	theme	sugar	861:865	arg1	antigens					867:874	sugar antigens	861:874	sugar antigens	861:874	RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens.					
31654087	5	27	theme	cell	724:727	arg1	lysates					729:735	cell lysates	724:735	cell lysates	724:735	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	8	28	theme	used	1031:1034	arg1	doses					1036:1040	All used doses	1027:1040	All used doses of RA	1027:1046	All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA.					
31654087	3	29	theme	many	371:374	arg1	activities					392:401	many pharmacological activities	371:401	many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic	371:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	29	theme	many	371:374	arg1	anti-bacterial					448:461	anti-bacterial	448:461	anti-bacterial	448:461	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	29	theme	many	371:374	arg1	anti-fibrotic					467:479	anti-fibrotic	467:479	anti-fibrotic	467:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	29	theme	many	371:374	arg1	anti-oxidative					432:445	anti-oxidative	432:445	anti-oxidative	432:445	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	29	theme	many	371:374	arg1	anti-inflammatory					413:429	anti-inflammatory	413:429	anti-inflammatory	413:429	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	9	30	theme	rosmarinic	1217:1226	arg1	acid					1228:1231	rosmarinic acid	1217:1231	rosmarinic acid	1217:1231	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	9	31	theme	sugar	1271:1275	arg1	antigens					1277:1284	specific sugar antigens	1262:1284	specific sugar antigens	1262:1284	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	3	32	theme	pharmacological	376:390	arg1	activities					392:401	many pharmacological activities	371:401	many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic	371:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	32	theme	pharmacological	376:390	arg1	anti-bacterial					448:461	anti-bacterial	448:461	anti-bacterial	448:461	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	32	theme	pharmacological	376:390	arg1	anti-fibrotic					467:479	anti-fibrotic	467:479	anti-fibrotic	467:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	32	theme	pharmacological	376:390	arg1	anti-oxidative					432:445	anti-oxidative	432:445	anti-oxidative	432:445	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	32	theme	pharmacological	376:390	arg1	anti-inflammatory					413:429	anti-inflammatory	413:429	anti-inflammatory	413:429	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	4	33	theme	acid	563:566	arg1	effect					523:528	the effect	519:528	the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts	519:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	5	34	theme	sugar	704:708	arg1	structures					710:719	sugar structures	704:719	sugar structures	704:719	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	2	35	theme	crucial	189:195	arg1	functions					208:216	many crucial biological functions	184:216	many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways	184:319	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	2	36	theme	pathways	312:319	arg1	differentiation					228:242	cell differentiation	223:242	cell differentiation	223:242	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	2	36	theme	pathways	312:319	arg1	adhesion					250:257	cell adhesion	245:257	cell adhesion	245:257	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	2	36	theme	pathways	312:319	arg1	interactions					270:281	cell-cell interactions	260:281	cell-cell interactions	260:281	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	2	36	theme	pathways	312:319	arg1	regulation					288:297	regulation	288:297	regulation of signaling pathways	288:319	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	0	37	theme	skin	35:38	arg1	fibroblasts					40:50	human skin fibroblasts	29:50	human skin fibroblasts	29:50	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	4	38	theme	rosmarinic	552:561	arg1	acid					563:566	25, 50, and 100 μM rosmarinic acid	533:566	25, 50, and 100 μM rosmarinic acid	533:566	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	5	39	theme	structures	710:719	arg1	level					695:699	the level	691:699	the level of sugar structures in cell lysates and culture supernatant	691:759	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	2	40	theme	many	184:187	arg1	functions					208:216	many crucial biological functions	184:216	many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways	184:319	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	0	41	gly	Glycosylation	0:12	arg1	proteins					17:24	proteins	17:24	proteins of human skin fibroblasts	17:50	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	0	42	theme	human	29:33	arg1	fibroblasts					40:50	human skin fibroblasts	29:50	human skin fibroblasts	29:50	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	2	43	theme	cell	245:248	arg1	adhesion					250:257	cell adhesion	245:257	cell adhesion	245:257	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	5	44	theme	culture	741:747	arg1	supernatant					749:759	culture supernatant	741:759	culture supernatant	741:759	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	5	45	used	used	676:679	arg2	test					641:644	ELISA-liked test	629:644	ELISA-liked test with biotinylated lectins	629:670	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	3	46	theme	Rosmarinic	322:331	arg1	flavonoid					356:364	a natural flavonoid	346:364	a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic	346:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	46	theme	Rosmarinic	322:331	arg1	RA					339:340	RA	339:340	RA	339:340	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	46	theme	Rosmarinic	322:331	arg1	acid					333:336	Rosmarinic acid	322:336	Rosmarinic acid (RA)	322:341	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	6	47	theme	antigens	867:874	arg1	formation					848:856	formation	848:856	formation of sugar antigens	848:874	RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens.					
31654087	3	48	with	flavonoid	356:364	arg1	activities					392:401	many pharmacological activities	371:401	many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic	371:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	48	with	flavonoid	356:364	arg1	anti-bacterial					448:461	anti-bacterial	448:461	anti-bacterial	448:461	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	48	with	flavonoid	356:364	arg1	anti-fibrotic					467:479	anti-fibrotic	467:479	anti-fibrotic	467:479	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	48	with	flavonoid	356:364	arg1	anti-oxidative					432:445	anti-oxidative	432:445	anti-oxidative	432:445	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	3	48	with	flavonoid	356:364	arg1	anti-inflammatory					413:429	anti-inflammatory	413:429	anti-inflammatory	413:429	Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic.					
31654087	1	49	theme	common	102:107	arg1	process					128:134	a common post-translational process	100:134	a common post-translational process of protein modification	100:158	Glycosylation is a common post-translational process of protein modification.					
31654087	1	49	theme	common	102:107	arg1	Glycosylation					83:95	Glycosylation	83:95	Glycosylation	83:95	Glycosylation is a common post-translational process of protein modification.					
31654087	0	50	theme	fibroblasts	40:50	arg1	proteins					17:24	proteins	17:24	proteins of human skin fibroblasts	17:50	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	9	51	theme	cell-cell	1394:1402	arg1	interactions					1404:1415	cell-cell interactions	1394:1415	cell-cell interactions	1394:1415	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	2	52	theme	cell	223:226	arg1	differentiation					228:242	cell differentiation	223:242	cell differentiation	223:242	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	7	53	theme	mannose	1009:1015	arg1	antigens					1017:1024	mannose antigens	1009:1024	mannose antigens	1009:1024	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	1	54	theme	post-translational	109:126	arg1	process					128:134	a common post-translational process	100:134	a common post-translational process of protein modification	100:158	Glycosylation is a common post-translational process of protein modification.					
31654087	1	54	theme	post-translational	109:126	arg1	Glycosylation					83:95	Glycosylation	83:95	Glycosylation	83:95	Glycosylation is a common post-translational process of protein modification.					
31654087	7	55	theme	T	925:925	arg1	antigens					927:934	T antigens	925:934	T antigens	925:934	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	9	56	from	effect	1252:1257	arg1	antigens					1277:1284	specific sugar antigens	1262:1284	specific sugar antigens	1262:1284	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	7	57	theme	antigens	927:934	arg1	expression					907:916	the expression	903:916	the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens	903:1024	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	4	58	theme	human	605:609	arg1	fibroblasts					616:626	human skin fibroblasts	605:626	human skin fibroblasts	605:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31654087	7	59	theme	Tn	921:922	arg1	expression					907:916	the expression	903:916	the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens	903:1024	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	5	60	from	level	695:699	arg1	lysates					729:735	cell lysates	724:735	cell lysates	724:735	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	5	60	from	level	695:699	arg1	supernatant					749:759	culture supernatant	741:759	culture supernatant	741:759	ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant.					
31654087	8	61	theme	GalNAcα2-6-sialyltransferase	1156:1183	arg1	mRNA					1185:1188	GalNAcα2-6-sialyltransferase mRNA	1156:1188	GalNAcα2-6-sialyltransferase mRNA	1156:1188	All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA.					
31654087	2	62	theme	biological	197:206	arg1	functions					208:216	many crucial biological functions	184:216	many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways	184:319	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	2	63	theme	cell-cell	260:268	arg1	interactions					270:281	cell-cell interactions	260:281	cell-cell interactions	260:281	Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways.					
31654087	1	64	theme	protein	139:145	arg1	modification					147:158	protein modification	139:158	protein modification	139:158	Glycosylation is a common post-translational process of protein modification.					
31654087	9	65	theme	crucial	1306:1312	arg1	functions					1327:1335	crucial carbohydrate functions	1306:1335	crucial carbohydrate functions	1306:1335	The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.					
31654087	0	66	theme	proteins	17:24	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of proteins of human skin fibroblasts	0:50	Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.					
31654087	7	67	theme	di	986:987	arg1	NAclactosamine					989:1002	di NAclactosamine	986:1002	di NAclactosamine	986:1002	Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens.					
31654087	1	68	theme	modification	147:158	arg1	process					128:134	a common post-translational process	100:134	a common post-translational process of protein modification	100:158	Glycosylation is a common post-translational process of protein modification.					
31654087	1	68	theme	modification	147:158	arg1	Glycosylation					83:95	Glycosylation	83:95	Glycosylation	83:95	Glycosylation is a common post-translational process of protein modification.					
31654087	8	69	theme	β1-3galactosyltransferase	1079:1103	arg1	mRNA					1105:1108	core 1 β1-3galactosyltransferase mRNA	1072:1108	core 1 β1-3galactosyltransferase mRNA	1072:1108	All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA.					
31654087	4	70	theme	skin	611:614	arg1	fibroblasts					616:626	human skin fibroblasts	605:626	human skin fibroblasts	605:626	In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts.					
31246420	0	0	theme	Detectable	80:89	arg1	Repertoire					100:109	the Complete Detectable O-Glycan Repertoire	67:109	the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	67:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	0	0	theme	Detectable	80:89	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	11	1	theme	O-glycan	2050:2057	arg1	samples					2059:2065	complex released O-glycan samples	2033:2065	complex released O-glycan samples	2033:2065	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	7	2	theme	core	1100:1103	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	9	3	with	effective	1361:1369	arg1	structures					1394:1403	labile sialylated structures	1376:1403	labile sialylated structures	1376:1403	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	9	4	theme	acid	1490:1493	arg1	B-fragment					1495:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	9	5	theme	sialylated	1383:1392	arg1	structures					1394:1403	labile sialylated structures	1376:1403	labile sialylated structures	1376:1403	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	0	6	theme	Complete	71:78	arg1	Repertoire					100:109	the Complete Detectable O-Glycan Repertoire	67:109	the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	67:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	0	6	theme	Complete	71:78	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	10	7	theme	290.1	1902:1906	arg1	fragment					1908:1915	the m/z 290.1 fragment	1894:1915	the m/z 290.1 fragment	1894:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	10	8	theme	m/z	1898:1900	arg1	fragment					1908:1915	the m/z 290.1 fragment	1894:1915	the m/z 290.1 fragment	1894:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	6	9	theme	produced	902:909	arg1	samples					911:917	biological and recombinantly produced samples	873:917	biological and recombinantly produced samples	873:917	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	4	10	gly	O-glycosylated	661:674	arg1	lubricin					684:691	the heavily O-glycosylated protein lubricin	649:691	the heavily O-glycosylated protein lubricin	649:691	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	6	11	theme	lubricin	806:813	arg1	potential					793:801	the therapeutic potential	777:801	the therapeutic potential of lubricin	777:813	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	8	12	theme	fragment	1321:1328	arg1	ions					1330:1333	the fragment ions	1317:1333	the fragment ions	1317:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	11	13	dep	quantification	2000:2013	arg1	the					1996:1998	the	1996:1998	the	1996:1998	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	0	14	theme	O-Glycan	91:98	arg1	Repertoire					100:109	the Complete Detectable O-Glycan Repertoire	67:109	the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	67:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	0	14	theme	O-Glycan	91:98	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	7	15	theme	sulfated	1015:1022	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	9	16	theme	sialic	1536:1541	arg1	isomers					1548:1554	sialic acid isomers	1536:1554	sialic acid isomers	1536:1554	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	11	17	theme	effective	1951:1959	arg1	approach					1983:1990	an effective and easily accessible approach	1948:1990	an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples	1948:2065	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	4	18	theme	protein	676:682	arg1	lubricin					684:691	the heavily O-glycosylated protein lubricin	649:691	the heavily O-glycosylated protein lubricin	649:691	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	1	19	theme	fundamental	170:180	arg1	modification					201:212	a fundamental post-translational modification	168:212	a fundamental post-translational modification	168:212	Glycosylation is a fundamental post-translational modification, occurring on half of all proteins.					
31246420	1	19	theme	fundamental	170:180	arg1	Glycosylation					151:163	Glycosylation	151:163	Glycosylation	151:163	Glycosylation is a fundamental post-translational modification, occurring on half of all proteins.					
31246420	0	20	theme	Monitoring	45:54	arg1	Method					56:61	a Multiple Reaction Monitoring Method	25:61	a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	25:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	6	21	theme	biological	873:882	arg1	samples					911:917	biological and recombinantly produced samples	873:917	biological and recombinantly produced samples	873:917	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	8	22	theme	effective	1190:1198	arg1	differentiation					1200:1214	effective differentiation	1190:1214	effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions	1190:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	6	23	theme	therapeutic	781:791	arg1	potential					793:801	the therapeutic potential	777:801	the therapeutic potential of lubricin	777:813	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	2	24	dep	branched	363:370	arg1	multi-isomer					373:384	multi-isomer	373:384	multi-isomer	373:384	Despite its significance, our understanding is limited, in part due to the inherent difficulty in studying these branched, multi-isomer structures.					
31246420	1	25	theme	post-translational	182:199	arg1	modification					201:212	a fundamental post-translational modification	168:212	a fundamental post-translational modification	168:212	Glycosylation is a fundamental post-translational modification, occurring on half of all proteins.					
31246420	1	25	theme	post-translational	182:199	arg1	Glycosylation					151:163	Glycosylation	151:163	Glycosylation	151:163	Glycosylation is a fundamental post-translational modification, occurring on half of all proteins.					
31246420	8	26	theme	Isomer-specific	1150:1164	arg1	transitions					1170:1180	Isomer-specific MRM transitions	1150:1180	Isomer-specific MRM transitions	1150:1180	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	5	27	theme	eye	766:768	arg1	lubrication					737:747	lubrication	737:747	lubrication of the joint and eye	737:768	Lubricin (proteoglycan 4) is essential for lubrication of the joint and eye.					
31246420	7	28	theme	core	1070:1073	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	7	29	theme	reductive	947:955	arg1	β-elimination					957:969	reductive β-elimination	947:969	reductive β-elimination	947:969	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	9	30	theme	acid	1543:1546	arg1	isomers					1548:1554	sialic acid isomers	1536:1554	sialic acid isomers	1536:1554	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	11	31	theme	complex	2033:2039	arg1	samples					2059:2065	complex released O-glycan samples	2033:2065	complex released O-glycan samples	2033:2065	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	3	32	theme	quantifiable	424:435	arg1	methods					437:443	Accessible, detailed, and quantifiable methods	398:443	Accessible, detailed, and quantifiable methods for studying glycans	398:464	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	3	32	theme	quantifiable	424:435	arg1	O-glycans					480:488	O-glycans	480:488	particularly O-glycans	467:488	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	3	33	theme	Accessible	398:407	arg1	methods					437:443	Accessible, detailed, and quantifiable methods	398:443	Accessible, detailed, and quantifiable methods for studying glycans	398:464	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	3	33	theme	Accessible	398:407	arg1	O-glycans					480:488	O-glycans	480:488	particularly O-glycans	467:488	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	7	34	theme	GlcNAcβ1-6	1116:1125	arg1	GalNAcα1-					1127:1135	Galβ1-3(GlcNAcβ1-6)GalNAcα1-	1108:1135	Galβ1-3(GlcNAcβ1-6)GalNAcα1-	1108:1135	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	7	34	theme	GlcNAcβ1-6	1116:1125	arg1	core					1070:1073	core 1	1070:1075	core 1 (Galβ1-3GalNAcα1-)	1070:1094	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	8	35	theme	glycan	1227:1232	arg1	isomers					1234:1240	neutral glycan isomers	1219:1240	neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions	1219:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	4	36	theme	O-glycosylated	661:674	arg1	lubricin					684:691	the heavily O-glycosylated protein lubricin	649:691	the heavily O-glycosylated protein lubricin	649:691	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	6	37	from	repertoire	859:868	arg1	samples					911:917	biological and recombinantly produced samples	873:917	biological and recombinantly produced samples	873:917	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	4	38	theme	MRM	548:550	arg1	approach					553:560	a multiple reaction monitoring (MRM) approach	516:560	a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans	516:624	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	0	39	theme	Therapeutic	128:138	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	10	40	with	isomers	1805:1811	arg1	Neu5Acα2-6					1822:1831	the Neu5Acα2-6	1818:1831	the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment	1818:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	10	41	theme	purchased	1681:1689	arg1	Neu5Acα2-3Galβ1-4Glc					1691:1710	purchased Neu5Acα2-3Galβ1-4Glc	1681:1710	purchased Neu5Acα2-3Galβ1-4Glc	1681:1710	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	8	42	theme	MRM	1166:1168	arg1	transitions					1170:1180	Isomer-specific MRM transitions	1150:1180	Isomer-specific MRM transitions	1150:1180	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	0	43	theme	Candidate	118:126	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	11	44	theme	accessible	1972:1981	arg1	approach					1983:1990	an effective and easily accessible approach	1948:1990	an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples	1948:2065	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	0	45	theme	Reaction	36:43	arg1	Method					56:61	a Multiple Reaction Monitoring Method	25:61	a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	25:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	0	46	theme	Lubricin	141:148	arg1	Repertoire					100:109	the Complete Detectable O-Glycan Repertoire	67:109	the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	67:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	0	46	theme	Lubricin	141:148	arg1	Lubricin					141:148	the Candidate Therapeutic, Lubricin	114:148	Lubricin	141:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	6	47	theme	O-glycan	850:857	arg1	repertoire					859:868	its O-glycan repertoire	846:868	its O-glycan repertoire in biological and recombinantly produced samples	846:917	Given the therapeutic potential of lubricin, it is essential to understand its O-glycan repertoire in biological and recombinantly produced samples.					
31246420	8	48	theme	isomers	1234:1240	arg1	differentiation					1200:1214	effective differentiation	1190:1214	effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions	1190:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	8	49	theme	isomeric	1262:1269	arg1	structures					1271:1280	sulfated isomeric structures	1253:1280	sulfated isomeric structures	1253:1280	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	0	50	theme	Multiple	27:34	arg1	Method					56:61	a Multiple Reaction Monitoring Method	25:61	a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin	25:148	Deciphering Isomers with a Multiple Reaction Monitoring Method for the Complete Detectable O-Glycan Repertoire of the Candidate Therapeutic, Lubricin.					
31246420	4	51	theme	reaction	527:534	arg1	approach					553:560	a multiple reaction monitoring (MRM) approach	516:560	a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans	516:624	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	5	52	dep	joint	756:760	arg1	the					752:754	the	752:754	the	752:754	Lubricin (proteoglycan 4) is essential for lubrication of the joint and eye.					
31246420	10	53	theme	optimal	1872:1878	arg1	generation					1880:1889	optimal generation	1872:1889	optimal generation of the m/z 290.1 fragment	1872:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	8	54	theme	sulfated	1253:1260	arg1	structures					1271:1280	sulfated isomeric structures	1253:1280	sulfated isomeric structures	1253:1280	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	4	55	theme	multiple	518:525	arg1	approach					553:560	a multiple reaction monitoring (MRM) approach	516:560	a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans	516:624	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	9	56	theme	sialic	1483:1488	arg1	B-fragment					1495:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	10	57	theme	fragment	1908:1915	arg1	generation					1880:1889	optimal generation	1872:1889	optimal generation of the m/z 290.1 fragment	1872:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	9	58	theme	optimal	1440:1446	arg1	energy					1458:1463	the optimal collision energy	1436:1463	the optimal collision energy for the m/z 290.1 sialic acid B-fragment	1436:1504	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	7	59	gly	sialylated	1025:1034	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	7	60	theme	sulfated	1046:1053	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	3	61	theme	detailed	410:417	arg1	methods					437:443	Accessible, detailed, and quantifiable methods	398:443	Accessible, detailed, and quantifiable methods for studying glycans	398:464	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	3	61	theme	detailed	410:417	arg1	O-glycans					480:488	O-glycans	480:488	particularly O-glycans	467:488	Accessible, detailed, and quantifiable methods for studying glycans, particularly O-glycans, are needed.					
31246420	10	62	theme	linkage	1797:1803	arg1	isomers					1805:1811	Neu5Acα2-6Galβ1-4GlcNAc linkage isomers	1773:1811	Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment	1773:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	10	63	with	Neu5Acα2-3Galβ1-4GlcNAc	1745:1767	arg1	Neu5Acα2-6					1822:1831	the Neu5Acα2-6	1818:1831	the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment	1818:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	8	64	theme	structures	1271:1280	arg1	differentiation					1200:1214	effective differentiation	1190:1214	effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions	1190:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	4	65	theme	monitoring	536:545	arg1	approach					553:560	a multiple reaction monitoring (MRM) approach	516:560	a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans	516:624	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	11	66	theme	samples	2059:2065	arg1	annotation					2019:2028	annotation	2019:2028	annotation	2019:2028	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	11	66	theme	samples	2059:2065	arg1	quantification					2000:2013	quantification	2000:2013	quantification	2000:2013	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	9	67	theme	fragmentation	1603:1615	arg1	spectra					1617:1623	fragmentation spectra	1603:1623	fragmentation spectra	1603:1623	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	7	68	theme	Galβ1-3	1108:1114	arg1	GalNAcα1-					1127:1135	Galβ1-3(GlcNAcβ1-6)GalNAcα1-	1108:1135	Galβ1-3(GlcNAcβ1-6)GalNAcα1-	1108:1135	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	7	68	theme	Galβ1-3	1108:1114	arg1	core					1070:1073	core 1	1070:1075	core 1 (Galβ1-3GalNAcα1-)	1070:1094	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	7	69	theme	neutral	1006:1012	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	2	70	theme	inherent	325:332	arg1	difficulty					334:343	the inherent difficulty	321:343	the inherent difficulty in studying these branched, multi-isomer structures	321:395	Despite its significance, our understanding is limited, in part due to the inherent difficulty in studying these branched, multi-isomer structures.					
31246420	4	71	theme	detectable	607:616	arg1	glycans					618:624	all detectable glycans	603:624	all detectable glycans	603:624	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	7	72	theme	sialylated	1025:1034	arg1	structures					1138:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	1003:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	4	73	from	approach	553:560	arg1	lubricin					684:691	the heavily O-glycosylated protein lubricin	649:691	the heavily O-glycosylated protein lubricin	649:691	Here we take a multiple reaction monitoring (MRM) approach to differentiate and relatively quantify all detectable glycans, including isomers, on the heavily O-glycosylated protein lubricin.					
31246420	9	74	theme	m/z	1473:1475	arg1	B-fragment					1495:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	the m/z 290.1 sialic acid B-fragment	1469:1504	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	1	75	theme	proteins	240:247	arg1	half					228:231	half	228:231	half of all proteins	228:247	Glycosylation is a fundamental post-translational modification, occurring on half of all proteins.					
31246420	9	76	theme	collision	1448:1456	arg1	energy					1458:1463	the optimal collision energy	1436:1463	the optimal collision energy for the m/z 290.1 sialic acid B-fragment	1436:1504	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	11	77	theme	released	2041:2048	arg1	samples					2059:2065	complex released O-glycan samples	2033:2065	complex released O-glycan samples	2033:2065	Overall, this method provides an effective and easily accessible approach for the quantification and annotation of complex released O-glycan samples.					
31246420	10	78	theme	Neu5Acα2-6Galβ1-4GlcNAc	1773:1795	arg1	isomers					1805:1811	Neu5Acα2-6Galβ1-4GlcNAc linkage isomers	1773:1811	Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment	1773:1915	This approach was also effective for purchased Neu5Acα2-3Galβ1-4Glc and Neu5Acα2-6Galβ1-4Glc and for Neu5Acα2-3Galβ1-4GlcNAc and Neu5Acα2-6Galβ1-4GlcNAc linkage isomers with the Neu5Acα2-6 consistently requiring more energy for optimal generation of the m/z 290.1 fragment.					
31246420	8	79	theme	neutral	1219:1225	arg1	isomers					1234:1240	neutral glycan isomers	1219:1240	neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions	1219:1333	Isomer-specific MRM transitions allowed effective differentiation of neutral glycan isomers as well as sulfated isomeric structures, where the sulfate was retained on the fragment ions.					
31246420	5	80	theme	joint	756:760	arg1	lubrication					737:747	lubrication	737:747	lubrication of the joint and eye	737:768	Lubricin (proteoglycan 4) is essential for lubrication of the joint and eye.					
31246420	9	81	gly	sialylated	1383:1392	arg1	structures					1394:1403	labile sialylated structures	1376:1403	labile sialylated structures	1376:1403	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31246420	2	82	theme	branched	363:370	arg1	structures					386:395	these branched, multi-isomer structures	357:395	these branched, multi-isomer structures	357:395	Despite its significance, our understanding is limited, in part due to the inherent difficulty in studying these branched, multi-isomer structures.					
31246420	7	83	theme	structures	1138:1147	arg1	range					994:998	a range	992:998	a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures	992:1147	O-Glycans were released by reductive β-elimination and defined, showing a range of 26 neutral, sulfated, sialylated, and both sulfated and sialylated core 1 (Galβ1-3GalNAcα1-) and core 2 (Galβ1-3(GlcNAcβ1-6)GalNAcα1-) structures.					
31246420	9	84	theme	labile	1376:1381	arg1	structures					1394:1403	labile sialylated structures	1376:1403	labile sialylated structures	1376:1403	This strategy was not as effective with labile sialylated structures; instead, it was observed that the optimal collision energy for the m/z 290.1 sialic acid B-fragment differed consistently between sialic acid isomers, allowing differentiation between isomers when fragmentation spectra were insufficient.					
31174130	6	0	theme	IgG	986:988	arg1	N-glycans					959:967	The released and derivatized N-glycans	930:967	The released and derivatized N-glycans of human plasma, IgG and human transferrin	930:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	2	1	gly	glycopeptides	447:459	arg2	glycopeptides					447:459	glycopeptides	447:459	glycopeptides	447:459	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	7	2	theme	enriched	1327:1334	arg1	samples					1336:1342	enriched samples	1327:1342	enriched samples	1327:1342	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	0	3	theme	efficient	65:73	arg1	analysis					75:82	efficient analysis	65:82	efficient analysis	65:82	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	1	4	gly	glycopeptides	171:183	arg2	glycopeptides					171:183	intact glycopeptides	164:183	intact glycopeptides	164:183	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	1	5	theme	intact	164:169	arg1	glycopeptides					171:183	intact glycopeptides	164:183	intact glycopeptides	164:183	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	8	6	theme	material	1411:1418	arg1	characterization					1387:1402	the structural characterization	1372:1402	the structural characterization of the material	1372:1418	Finally, the structural characterization of the material was achieved in detail using various characterization approaches.					
31174130	0	7	theme	glycopeptides	87:99	arg1	enrichment					50:59	fast enrichment	45:59	fast enrichment	45:59	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	0	7	theme	glycopeptides	87:99	arg1	analysis					75:82	efficient analysis	65:82	efficient analysis	65:82	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	6	8	theme	plasma	978:983	arg1	N-glycans					959:967	The released and derivatized N-glycans	930:967	The released and derivatized N-glycans of human plasma, IgG and human transferrin	930:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	3	9	theme	N-glycopeptides	588:602	arg1	enrichment					570:579	the enrichment	566:579	the enrichment of IgG N-glycopeptides from their proteolytic products	566:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	3	10	theme	proteolytic	615:625	arg1	products					627:634	their proteolytic products	609:634	their proteolytic products	609:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	1	11	theme	analytical	292:301	arg1	challenges					303:312	analytical challenges	292:312	analytical challenges observed in the analysis	292:337	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	6	12	theme	human	972:976	arg1	plasma					978:983	human plasma	972:983	human plasma	972:983	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	7	13	theme	samples	1336:1342	arg1	analysis					1315:1322	the analysis	1311:1322	the analysis of enriched samples	1311:1342	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	1	14	located	observed	314:321	arg2	challenges					303:312	analytical challenges	292:312	analytical challenges observed in the analysis	292:337	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	1	14	located	observed	314:321	arg1	analysis					330:337	the analysis	326:337	the analysis	326:337	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	1	15	theme	challenges	303:312	arg1	glycomics					271:279	glycomics	271:279	glycomics	271:279	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	1	15	theme	challenges	303:312	arg1	glycoproteomics					251:265	mass spectrometry-based glycoproteomics	227:265	mass spectrometry-based glycoproteomics	227:265	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	0	16	theme	glycans	105:111	arg1	enrichment					50:59	fast enrichment	45:59	fast enrichment	45:59	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	0	16	theme	glycans	105:111	arg1	analysis					75:82	efficient analysis	65:82	efficient analysis	65:82	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	7	17	theme	human	1186:1190	arg1	sample					1230:1235	a real-world complex sample	1209:1235	a real-world complex sample	1209:1235	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	17	theme	human	1186:1190	arg1	digest					1199:1204	human plasma digest	1186:1204	human plasma digest	1186:1204	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	3	18	from	enrichment	570:579	arg1	products					627:634	their proteolytic products	609:634	their proteolytic products	609:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	7	19	gly	glycoproteins	1280:1292	arg1	glycoproteins					1280:1292	human plasma glycoproteins	1267:1292	human plasma glycoproteins	1267:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	2	20	theme	efficient	425:433	arg1	analysis					435:442	the efficient analysis	421:442	the efficient analysis of glycopeptides and glycans	421:471	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	7	21	theme	plasma	1192:1197	arg1	sample					1230:1235	a real-world complex sample	1209:1235	a real-world complex sample	1209:1235	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	21	theme	plasma	1192:1197	arg1	digest					1199:1204	human plasma digest	1186:1204	human plasma digest	1186:1204	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	3	22	theme	IgG	584:586	arg1	N-glycopeptides					588:602	IgG N-glycopeptides	584:602	IgG N-glycopeptides from their proteolytic products	584:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	3	23	theme	glycopeptide	478:489	arg1	efficacy					502:509	The glycopeptide enrichment efficacy	474:509	The glycopeptide enrichment efficacy of the titania-based sol-gel material	474:547	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	0	24	theme	New	2:4	arg1	glyco-purification					14:31	A New titania glyco-purification	0:31	A New titania glyco-purification	0:31	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	6	25	theme	derivatized	947:957	arg1	N-glycans					959:967	The released and derivatized N-glycans	930:967	The released and derivatized N-glycans of human plasma, IgG and human transferrin	930:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	4	26	theme	material	714:721	arg1	sensitivity					657:667	sensitivity	657:667	sensitivity	657:667	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	4	26	theme	material	714:721	arg1	μL-1					679:682	0.5 fmol μL-1	670:682	0.5 fmol μL-1	670:682	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	4	26	theme	material	714:721	arg1	selectivity					641:651	selectivity	641:651	selectivity	641:651	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	1	27	theme	derivatized	189:199	arg1	glycans					201:207	derivatized glycans	189:207	derivatized glycans	189:207	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	7	28	theme	human	1267:1271	arg1	glycoproteins					1280:1292	human plasma glycoproteins	1267:1292	human plasma glycoproteins	1267:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	6	29	theme	glyco-purification	1050:1067	arg1	tips					1069:1072	titania glyco-purification tips	1042:1072	titania glyco-purification tips for the analysis by MALDI-MS	1042:1101	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	4	30	theme	commercial	769:778	arg1	TiO2					780:783	commercial TiO2	769:783	commercial TiO2	769:783	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	0	31	gly	glycopeptides	87:99	arg2	glycopeptides					87:99	glycopeptides	87:99	glycopeptides	87:99	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	8	32	theme	characterization	1457:1472	arg1	approaches					1474:1483	various characterization approaches	1449:1483	various characterization approaches	1449:1483	Finally, the structural characterization of the material was achieved in detail using various characterization approaches.					
31174130	3	33	gly	N-glycopeptides	588:602	arg1	products					627:634	their proteolytic products	609:634	their proteolytic products	609:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	3	33	gly	N-glycopeptides	588:602	arg2	N-glycopeptides					588:602	IgG N-glycopeptides	584:602	IgG N-glycopeptides from their proteolytic products	584:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	5	34	theme	titania-based	884:896	arg1	material					898:905	titania-based material	884:905	titania-based material into the pipette tips	884:927	A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips.					
31174130	5	35	theme	pipette	916:922	arg1	tips					924:927	the pipette tips	912:927	the pipette tips	912:927	A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips.					
31174130	7	36	theme	tip	1165:1167	arg1	performance					1119:1129	The enrichment performance	1104:1129	The enrichment performance of the titania glyco-purification tip	1104:1167	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	37	dep	tested	1173:1178	arg1	detected					1299:1306	detected	1299:1306	were detected by the analysis of enriched samples by nLC-QTOF-MS/MS	1294:1360	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	2	38	theme	glycans	465:471	arg1	analysis					435:442	the efficient analysis	421:442	the efficient analysis of glycopeptides and glycans	421:471	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	4	39	theme	0.5 fmol	670:677	arg1	μL-1					679:682	0.5 fmol μL-1	670:682	0.5 fmol μL-1	670:682	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	4	39	theme	0.5 fmol	670:677	arg1	selectivity					641:651	selectivity	641:651	selectivity	641:651	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	0	40	dep	enrichment	50:59	arg1	the					41:43	the	41:43	the	41:43	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	2	41	theme	glycopeptides	447:459	arg1	analysis					435:442	the efficient analysis	421:442	the efficient analysis of glycopeptides and glycans	421:471	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	5	42	theme	glycomic	841:848	arg1	analysis					850:857	rapid glycoproteomic and glycomic analysis	816:857	analysis	850:857	A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips.					
31174130	7	43	theme	titania	1138:1144	arg1	tip					1165:1167	the titania glyco-purification tip	1134:1167	the titania glyco-purification tip	1134:1167	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	4	44	theme	sol-gel	706:712	arg1	material					714:721	the titania-based sol-gel material	688:721	the titania-based sol-gel material	688:721	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	3	45	theme	enrichment	491:500	arg1	efficacy					502:509	The glycopeptide enrichment efficacy	474:509	The glycopeptide enrichment efficacy of the titania-based sol-gel material	474:547	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	1	46	theme	glycopeptides	171:183	arg1	Enrichment					130:139	Enrichment	130:139	Enrichment	130:139	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	1	46	theme	glycopeptides	171:183	arg1	glycans					201:207	derivatized glycans	189:207	derivatized glycans	189:207	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	7	47	theme	glyco-purification	1146:1163	arg1	tip					1165:1167	the titania glyco-purification tip	1134:1167	the titania glyco-purification tip	1134:1167	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	4	48	dep	selectivity	641:651	arg1	The					637:639	The	637:639	The	637:639	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	4	49	theme	titania-based	692:704	arg1	material					714:721	the titania-based sol-gel material	688:721	the titania-based sol-gel material	688:721	The selectivity and sensitivity (0.5 fmol μL-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2.					
31174130	0	50	theme	fast	45:48	arg1	enrichment					50:59	fast enrichment	45:59	fast enrichment	45:59	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	6	51	theme	titania	1042:1048	arg1	tips					1069:1072	titania glyco-purification tips	1042:1072	titania glyco-purification tips for the analysis by MALDI-MS	1042:1101	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	3	52	theme	material	540:547	arg1	efficacy					502:509	The glycopeptide enrichment efficacy	474:509	The glycopeptide enrichment efficacy of the titania-based sol-gel material	474:547	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	7	53	from	glycoproteins	1280:1292	arg1	N-glycopeptides					1246:1260	112 N-glycopeptides	1242:1260	112 N-glycopeptides from human plasma glycoproteins	1242:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	6	54	theme	transferrin	1000:1010	arg1	N-glycans					959:967	The released and derivatized N-glycans	930:967	The released and derivatized N-glycans of human plasma, IgG and human transferrin	930:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	7	55	theme	real-world	1211:1220	arg1	sample					1230:1235	a real-world complex sample	1209:1235	a real-world complex sample	1209:1235	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	55	theme	real-world	1211:1220	arg1	digest					1199:1204	human plasma digest	1186:1204	human plasma digest	1186:1204	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	8	56	theme	structural	1376:1385	arg1	characterization					1387:1402	the structural characterization	1372:1402	the structural characterization of the material	1372:1418	Finally, the structural characterization of the material was achieved in detail using various characterization approaches.					
31174130	6	57	theme	human	994:998	arg1	transferrin					1000:1010	human transferrin	994:1010	human transferrin	994:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	7	58	theme	enrichment	1108:1117	arg1	performance					1119:1129	The enrichment performance	1104:1129	The enrichment performance of the titania glyco-purification tip	1104:1167	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	3	59	gly	glycopeptide	478:489	arg2	glycopeptide					478:489	glycopeptide	478:489	glycopeptide	478:489	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	3	60	theme	titania-based	518:530	arg1	material					540:547	the titania-based sol-gel material	514:547	the titania-based sol-gel material	514:547	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	1	61	theme	spectrometry-based	232:249	arg1	glycoproteomics					251:265	mass spectrometry-based glycoproteomics	227:265	mass spectrometry-based glycoproteomics	227:265	Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis.					
31174130	8	62	theme	various	1449:1455	arg1	approaches					1474:1483	various characterization approaches	1449:1483	various characterization approaches	1449:1483	Finally, the structural characterization of the material was achieved in detail using various characterization approaches.					
31174130	2	63	theme	titania-based	348:360	arg1	material					362:369	a titania-based material	346:369	a titania-based material	346:369	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	3	64	from	products	627:634	arg1	N-glycopeptides					588:602	IgG N-glycopeptides	584:602	IgG N-glycopeptides from their proteolytic products	584:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	3	64	from	products	627:634	arg1	enrichment					570:579	the enrichment	566:579	the enrichment of IgG N-glycopeptides from their proteolytic products	566:634	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	6	65	theme	released	934:941	arg1	N-glycans					959:967	The released and derivatized N-glycans	930:967	The released and derivatized N-glycans of human plasma, IgG and human transferrin	930:1010	The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS.					
31174130	7	66	gly	N-glycopeptides	1246:1260	arg2	N-glycopeptides					1246:1260	112 N-glycopeptides	1242:1260	112 N-glycopeptides from human plasma glycoproteins	1242:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	66	gly	N-glycopeptides	1246:1260	arg1	glycoproteins					1280:1292	human plasma glycoproteins	1267:1292	human plasma glycoproteins	1267:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	2	67	theme	sol-gel	402:408	arg1	method					410:415	a facile sol-gel method	393:415	a facile sol-gel method for the efficient analysis of glycopeptides and glycans	393:471	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	3	68	theme	sol-gel	532:538	arg1	material					540:547	the titania-based sol-gel material	514:547	the titania-based sol-gel material	514:547	The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products.					
31174130	7	69	theme	complex	1222:1228	arg1	sample					1230:1235	a real-world complex sample	1209:1235	a real-world complex sample	1209:1235	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	69	theme	complex	1222:1228	arg1	digest					1199:1204	human plasma digest	1186:1204	human plasma digest	1186:1204	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	7	70	theme	plasma	1273:1278	arg1	glycoproteins					1280:1292	human plasma glycoproteins	1267:1292	human plasma glycoproteins	1267:1292	The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS.					
31174130	0	71	theme	titania	6:12	arg1	glyco-purification					14:31	A New titania glyco-purification	0:31	A New titania glyco-purification	0:31	A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS.					
31174130	2	72	theme	facile	395:400	arg1	method					410:415	a facile sol-gel method	393:415	a facile sol-gel method for the efficient analysis of glycopeptides and glycans	393:471	Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans.					
31174130	5	73	theme	pipette-tip	788:798	arg1	application					800:810	A pipette-tip application	786:810	A pipette-tip application for rapid glycoproteomic and glycomic analysis	786:857	A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips.					
30157178	4	0	theme	Envs	547:550	arg1	mass					524:527	mass	524:527	mass	524:527	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	4	0	theme	Envs	547:550	arg1	charge					537:542	net charge	533:542	net charge	533:542	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	7	1	theme	cell	1113:1116	arg1	line					1118:1121	This cell line	1108:1121	This cell line	1108:1121	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	2	2	theme	amino	317:321	arg1	acid					323:326	both amino acid and glycan residues	312:346	acid	323:326	Many of these recognize epitopes consisting of both amino acid and glycan residues.					
30157178	8	3	theme	epitopes	1679:1686	arg1	structure					1633:1641	the structure	1629:1641	the structure of the important glycan-independent epitopes (e.g., VRC01)	1629:1700	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	1	4	theme	Env	233:235	arg1	gp120					238:242	the HIV-1 envelope protein (Env) gp120	205:242	the HIV-1 envelope protein (Env) gp120	205:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	9	5	theme	immunogens	2003:2012	arg1	production					1975:1984	the biopharmaceutical production	1953:1984	the biopharmaceutical production of HIV-1 vaccine immunogens	1953:2012	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	4	6	theme	mature	623:628	arg1	carbohydrates					664:676	mature, complex (sialic acid-containing) carbohydrates	623:676	mature, complex (sialic acid-containing) carbohydrates	623:676	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	7	7	theme	interspaced	1165:1175	arg1	repeat					1195:1200	the clustered regularly interspaced short palindromic repeat	1141:1200	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	7	theme	interspaced	1165:1175	arg1	CRISPR					1203:1208	CRISPR	1203:1208	CRISPR	1203:1208	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	1	8	theme	neutralizing	157:168	arg1	bN-mAbs					193:199	bN-mAbs	193:199	bN-mAbs	193:199	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	1	8	theme	neutralizing	157:168	arg1	antibodies					181:190	multiple broadly neutralizing monoclonal antibodies	140:190	multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120	140:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	9	9	theme	high	1885:1888	arg1	densities					1895:1903	high cell densities	1885:1903	high cell densities	1885:1903	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	2	10	theme	these	273:277	arg1	these					273:277	these	273:277	these	273:277	Many of these recognize epitopes consisting of both amino acid and glycan residues.					
30157178	2	10	theme	these	273:277	arg1	Many					265:268	Many	265:268	Many	265:268	Many of these recognize epitopes consisting of both amino acid and glycan residues.					
30157178	4	11	theme	acid	594:597	arg1	sequence					599:606	the same amino acid sequence	579:606	the same amino acid sequence	579:606	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	8	12	theme	CHO	1450:1452	arg1	line					1459:1462	the MGAT1- CHO cell line	1439:1462	the MGAT1- CHO cell line	1439:1462	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	9	13	link	N-linked	1796:1803	arg1	pathway					1819:1825	the N-linked glycosylation pathway	1792:1825	the N-linked glycosylation pathway	1792:1825	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	5	14	theme	cell	685:688	arg1	lines					690:694	cell lines	685:694	cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates	685:815	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	7	15	theme	-Glycoprotein	1346:1358	arg1	Beta-1,2-N-Acetylglucosaminyltransferase					1360:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase	1325:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1)	1325:1407	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	15	theme	-Glycoprotein	1346:1358	arg1	MGAT1					1402:1406	MGAT1	1402:1406	MGAT1	1402:1406	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	4	16	with	molecules	564:572	arg1	sequence					599:606	the same amino acid sequence	579:606	the same amino acid sequence	579:606	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	5	17	theme	glycan-dependent	898:913	arg1	epitopes					915:922	these glycan-dependent epitopes	892:922	these glycan-dependent epitopes	892:922	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	8	18	theme	improved	1472:1479	arg1	binding					1481:1487	improved binding	1472:1487	improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074)	1472:1610	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	7	19	theme	Alpha-1,3-	1335:1344	arg1	Beta-1,2-N-Acetylglucosaminyltransferase					1360:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase	1325:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1)	1325:1407	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	19	theme	Alpha-1,3-	1335:1344	arg1	MGAT1					1402:1406	MGAT1	1402:1406	MGAT1	1402:1406	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	6	20	theme	hamster	1017:1023	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	7	21	theme	Mannosyl	1325:1332	arg1	Beta-1,2-N-Acetylglucosaminyltransferase					1360:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase	1325:1399	Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1)	1325:1407	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	21	theme	Mannosyl	1325:1332	arg1	MGAT1					1402:1406	MGAT1	1402:1406	MGAT1	1402:1406	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	22	theme	protein	1229:1235	arg1	gene					1246:1249	/CRISPR-associated protein 9 (Cas9) gene	1210:1249	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	5	23	theme	earlier	795:801	arg1	intermediates					803:815	earlier intermediates	795:815	earlier intermediates	795:815	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	5	24	theme	vaccine	862:868	arg1	immunogens					870:879	vaccine immunogens	862:879	vaccine immunogens displaying these glycan-dependent epitopes	862:922	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	6	25	theme	suspension-adapted	990:1007	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	9	26	theme	MGAT1-	1722:1727	arg1	line					1738:1741	the MGAT1- CHO cell line	1718:1741	the MGAT1- CHO cell line	1718:1741	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	8	27	theme	glycan-dependent	1503:1518	arg1	bN-mAbs					1520:1526	prototypic glycan-dependent bN-mAbs	1492:1526	prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074)	1492:1610	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	0	28	theme	cell	68:71	arg1	line					73:76	an MGAT1-deficient CHO cell line	45:76	an MGAT1-deficient CHO cell line	45:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	1	29	theme	multiple	140:147	arg1	bN-mAbs					193:199	bN-mAbs	193:199	bN-mAbs	193:199	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	1	29	theme	multiple	140:147	arg1	antibodies					181:190	multiple broadly neutralizing monoclonal antibodies	140:190	multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120	140:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	9	30	theme	cell	1733:1736	arg1	line					1738:1741	the MGAT1- CHO cell line	1718:1741	the MGAT1- CHO cell line	1718:1741	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	9	31	dep	ability	1707:1713	arg1	limit					1746:1750	limit	1746:1750	to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities	1743:1903	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	5	32	theme	suitable	696:703	arg1	lines					690:694	cell lines	685:694	cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates	685:815	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	8	33	dep	PGT128	1592:1597	arg1	e.g.					1586:1589	e.g.	1586:1589	e.g.	1586:1589	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	9	34	theme	HIV-1	1989:1993	arg1	immunogens					2003:2012	HIV-1 vaccine immunogens	1989:2012	HIV-1 vaccine immunogens	1989:2012	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	5	35	theme	biopharmaceutical	709:725	arg1	production					727:736	biopharmaceutical production	709:736	biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates	709:815	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	0	36	theme	CHO	64:66	arg1	line					73:76	an MGAT1-deficient CHO cell line	45:76	an MGAT1-deficient CHO cell line	45:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	0	37	theme	CRISPR/Cas9	0:10	arg1	editing					17:23	CRISPR/Cas9 gene editing	0:23	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line	0:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	6	38	theme	line	1042:1045	arg1	development					966:976	the development	962:976	the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	962:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	9	39	theme	early	1769:1773	arg1	intermediates					1775:1787	early intermediates	1769:1787	early intermediates	1769:1787	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	7	40	theme	clustered	1145:1153	arg1	repeat					1195:1200	the clustered regularly interspaced short palindromic repeat	1141:1200	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	40	theme	clustered	1145:1153	arg1	CRISPR					1203:1208	CRISPR	1203:1208	CRISPR	1203:1208	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	4	41	dep	carbohydrates	664:676	arg1	acid-containing					647:661	acid-containing	647:661	acid-containing	647:661	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	4	42	theme	net	533:535	arg1	charge					537:542	net charge	533:542	net charge	533:542	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	9	43	theme	N-linked	1796:1803	arg1	pathway					1819:1825	the N-linked glycosylation pathway	1792:1825	the N-linked glycosylation pathway	1792:1825	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	4	44	theme	glycosylation	485:497	arg1	type					477:480	This type	472:480	This type of glycosylation	472:497	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	0	45	theme	MGAT1-deficient	48:62	arg1	line					73:76	an MGAT1-deficient CHO cell line	45:76	an MGAT1-deficient CHO cell line	45:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	3	46	theme	glycosylation	449:461	arg1	pathway					463:469	the N-linked glycosylation pathway	436:469	the N-linked glycosylation pathway	436:469	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	8	47	theme	V3	1577:1578	arg1	stem					1580:1583	the V3 stem	1573:1583	the V3 stem (e.g., PGT128 and 10-1074)	1573:1610	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	7	48	dep	repeat	1195:1200	arg1	gene					1246:1249	/CRISPR-associated protein 9 (Cas9) gene	1210:1249	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	3	49	theme	early	413:417	arg1	glycans					363:369	the glycans	359:369	the glycans required for binding of these bN-mAbs	359:407	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	3	49	theme	early	413:417	arg1	intermediates					419:431	early intermediates	413:431	early intermediates in the N-linked glycosylation pathway	413:469	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	8	50	theme	MGAT1-	1443:1448	arg1	line					1459:1462	the MGAT1- CHO cell line	1439:1462	the MGAT1- CHO cell line	1439:1462	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	2	51	theme	glycan	332:337	arg1	residues					339:346	both amino acid and glycan residues	312:346	residues	339:346	Many of these recognize epitopes consisting of both amino acid and glycan residues.					
30157178	1	52	theme	envelope	215:222	arg1	gp120					238:242	the HIV-1 envelope protein (Env) gp120	205:242	the HIV-1 envelope protein (Env) gp120	205:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	7	53	theme	short	1177:1181	arg1	repeat					1195:1200	the clustered regularly interspaced short palindromic repeat	1141:1200	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	53	theme	short	1177:1181	arg1	CRISPR					1203:1208	CRISPR	1203:1208	CRISPR	1203:1208	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	9	54	theme	doubling	1849:1856	arg1	time					1858:1861	the doubling time	1845:1861	the doubling time	1845:1861	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	8	55	theme	glycan-independent	1660:1677	arg1	epitopes					1679:1686	the important glycan-independent epitopes	1646:1686	the important glycan-independent epitopes (e.g., VRC01)	1646:1700	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	1	56	theme	broadly	149:155	arg1	bN-mAbs					193:199	bN-mAbs	193:199	bN-mAbs	193:199	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	1	56	theme	broadly	149:155	arg1	antibodies					181:190	multiple broadly neutralizing monoclonal antibodies	140:190	multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120	140:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	9	57	from	glycosylation	1752:1764	arg1	pathway					1819:1825	the N-linked glycosylation pathway	1792:1825	the N-linked glycosylation pathway	1792:1825	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	5	58	theme	N-linked	749:756	arg1	glycosylation					758:770	N-linked glycosylation	749:770	N-linked glycosylation	749:770	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	1	59	theme	monoclonal	170:179	arg1	bN-mAbs					193:199	bN-mAbs	193:199	bN-mAbs	193:199	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	1	59	theme	monoclonal	170:179	arg1	antibodies					181:190	multiple broadly neutralizing monoclonal antibodies	140:190	multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120	140:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	9	60	theme	cell	1890:1893	arg1	densities					1895:1903	high cell densities	1885:1903	high cell densities	1885:1903	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	5	61	link	N-linked	749:756	arg1	glycosylation					758:770	N-linked glycosylation	749:770	N-linked glycosylation	749:770	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	8	62	theme	cell	1454:1457	arg1	line					1459:1462	the MGAT1- CHO cell line	1439:1462	the MGAT1- CHO cell line	1439:1462	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	4	63	theme	amino	588:592	arg1	sequence					599:606	the same amino acid sequence	579:606	the same amino acid sequence	579:606	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	3	64	link	N-linked	440:447	arg1	pathway					463:469	the N-linked glycosylation pathway	436:469	the N-linked glycosylation pathway	436:469	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	8	65	dep	PG9	1564:1566	arg1	e.g.					1558:1561	e.g.	1558:1561	e.g.	1558:1561	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	6	66	theme	ovary	1025:1029	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	8	67	theme	prototypic	1492:1501	arg1	bN-mAbs					1520:1526	prototypic glycan-dependent bN-mAbs	1492:1526	prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074)	1492:1610	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	9	68	theme	useful	1932:1937	arg1	substrate					1939:1947	a useful substrate	1930:1947	a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens	1930:2012	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	9	68	theme	useful	1932:1937	arg1	it					1919:1920	it	1919:1920	it	1919:1920	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	7	69	theme	/CRISPR-associated	1210:1227	arg1	gene					1246:1249	/CRISPR-associated protein 9 (Cas9) gene	1210:1249	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	1	70	theme	last	127:130	arg1	decade					132:137	the last decade	123:137	the last decade	123:137	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	6	71	theme	Chinese	1009:1015	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	9	72	theme	line	1738:1741	arg1	ability					1707:1713	The ability	1703:1713	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities	1703:1903	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	0	73	theme	line	73:76	arg1	creation					33:40	the creation	29:40	the creation of an MGAT1-deficient CHO cell line	29:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	5	74	theme	immunogens	870:879	arg1	production					848:857	the production	844:857	the production of vaccine immunogens displaying these glycan-dependent epitopes	844:922	Since cell lines suitable for biopharmaceutical production that limit N-linked glycosylation to mannose-5 (Man5) or earlier intermediates are not readily available, the production of vaccine immunogens displaying these glycan-dependent epitopes has been challenging.					
30157178	6	75	theme	stable	983:988	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	9	76	theme	CHO	1729:1731	arg1	line					1738:1741	the MGAT1- CHO cell line	1718:1741	the MGAT1- CHO cell line	1718:1741	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	9	77	theme	biopharmaceutical	1957:1973	arg1	production					1975:1984	the biopharmaceutical production	1953:1984	the biopharmaceutical production of HIV-1 vaccine immunogens	1953:2012	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	9	78	gly	glycosylation	1752:1764	arg1	pathway					1819:1825	the N-linked glycosylation pathway	1792:1825	the N-linked glycosylation pathway	1792:1825	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	7	79	theme	palindromic	1183:1193	arg1	repeat					1195:1200	the clustered regularly interspaced short palindromic repeat	1141:1200	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	7	79	theme	palindromic	1183:1193	arg1	CRISPR					1203:1208	CRISPR	1203:1208	CRISPR	1203:1208	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	8	80	theme	V1/V2	1544:1548	arg1	PG9					1564:1566	PG9	1564:1566	PG9	1564:1566	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	8	80	theme	V1/V2	1544:1548	arg1	domain					1550:1555	the V1/V2 domain	1540:1555	the V1/V2 domain (e.g., PG9)	1540:1567	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	0	81	theme	vaccine	95:101	arg1	glycosylation					103:115	HIV-1 vaccine glycosylation	89:115	HIV-1 vaccine glycosylation	89:115	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	8	82	dep	VRC01	1695:1699	arg1	e.g.					1689:1692	e.g.	1689:1692	e.g.	1689:1692	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	3	83	theme	bN-mAbs	401:407	arg1	binding					384:390	binding	384:390	binding of these bN-mAbs	384:407	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	0	84	theme	gene	12:15	arg1	editing					17:23	CRISPR/Cas9 gene editing	0:23	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line	0:76	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	4	85	dep	mass	524:527	arg1	the					520:522	the	520:522	the	520:522	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	6	86	theme	cell	1037:1040	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	6	87	theme	earlier	1085:1091	arg1	intermediates					1093:1105	earlier intermediates	1085:1105	earlier intermediates	1085:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	6	88	theme	CHO	1032:1034	arg1	line					1042:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line	981:1045	a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates	981:1105	Here, we report the development of a stable suspension-adapted Chinese hamster ovary (CHO) cell line that limits glycosylation to Man5 and earlier intermediates.					
30157178	0	89	theme	HIV-1	89:93	arg1	glycosylation					103:115	HIV-1 vaccine glycosylation	89:115	HIV-1 vaccine glycosylation	89:115	CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.					
30157178	9	90	theme	glycosylation	1805:1817	arg1	pathway					1819:1825	the N-linked glycosylation pathway	1792:1825	the N-linked glycosylation pathway	1792:1825	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	8	91	theme	Monomeric	1410:1418	arg1	gp120s					1420:1425	Monomeric gp120s	1410:1425	Monomeric gp120s produced in the MGAT1- CHO cell line	1410:1462	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	3	92	from	intermediates	419:431	arg1	pathway					463:469	the N-linked glycosylation pathway	436:469	the N-linked glycosylation pathway	436:469	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	9	93	theme	vaccine	1995:2001	arg1	immunogens					2003:2012	HIV-1 vaccine immunogens	1989:2012	HIV-1 vaccine immunogens	1989:2012	The ability of the MGAT1- CHO cell line to limit glycosylation to early intermediates in the N-linked glycosylation pathway without impairing the doubling time or ability to grow at high cell densities suggests that it will be a useful substrate for the biopharmaceutical production of HIV-1 vaccine immunogens.					
30157178	3	94	theme	N-linked	440:447	arg1	pathway					463:469	the N-linked glycosylation pathway	436:469	the N-linked glycosylation pathway	436:469	Moreover, the glycans required for binding of these bN-mAbs are early intermediates in the N-linked glycosylation pathway.					
30157178	1	95	theme	HIV-1	209:213	arg1	gp120					238:242	the HIV-1 envelope protein (Env) gp120	205:242	the HIV-1 envelope protein (Env) gp120	205:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30157178	4	96	dep	mature	623:628	arg1	complex					631:637	complex	631:637	complex	631:637	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	7	97	theme	Cas9	1240:1243	arg1	gene					1246:1249	/CRISPR-associated protein 9 (Cas9) gene	1210:1249	the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system	1141:1264	This cell line was created using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing system and contains a mutation that inactivates the gene encoding Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1).					
30157178	4	98	theme	same	583:586	arg1	sequence					599:606	the same amino acid sequence	579:606	the same amino acid sequence	579:606	This type of glycosylation substantially alters the mass and net charge of Envs compared to molecules with the same amino acid sequence but possessing mature, complex (sialic acid-containing) carbohydrates.					
30157178	8	99	theme	important	1650:1658	arg1	epitopes					1679:1686	the important glycan-independent epitopes	1646:1686	the important glycan-independent epitopes (e.g., VRC01)	1646:1700	Monomeric gp120s produced in the MGAT1- CHO cell line exhibit improved binding to prototypic glycan-dependent bN-mAbs directed to the V1/V2 domain (e.g., PG9) and the V3 stem (e.g., PGT128 and 10-1074) while preserving the structure of the important glycan-independent epitopes (e.g., VRC01).					
30157178	1	100	theme	protein	224:230	arg1	gp120					238:242	the HIV-1 envelope protein (Env) gp120	205:242	the HIV-1 envelope protein (Env) gp120	205:242	Over the last decade, multiple broadly neutralizing monoclonal antibodies (bN-mAbs) to the HIV-1 envelope protein (Env) gp120 have been described.					
30151520	4	0	theme	glycosylation	901:913	arg1	site					915:918	glycosylation site	901:918	glycosylation site	901:918	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	8	1	theme	CID	1755:1757	arg1	energies					1759:1766	CID energies	1755:1766	CID energies	1755:1766	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	7	2	theme	glycopeptide	1540:1551	arg1	ions					1553:1556	glycopeptide ions	1540:1556	glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics	1540:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	7	3	dep	composition	1577:1587	arg1	e.g.					1559:1562	e.g.	1559:1562	e.g.	1559:1562	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	4	4	theme	polypeptide	875:885	arg1	sequence					887:894	polypeptide sequence	875:894	polypeptide sequence	875:894	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	1	5	theme	tandem	198:203	arg1	MS/MS					224:228	MS/MS	224:228	MS/MS	224:228	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	1	5	theme	tandem	198:203	arg1	spectrometry					210:221	tandem mass spectrometry	198:221	tandem mass spectrometry (MS/MS)	198:229	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	7	6	theme	other	1504:1508	arg1	properties					1526:1535	other physicochemical properties	1504:1535	other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics	1504:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	3	7	theme	type	787:790	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	6	8	theme	collision	1266:1274	arg1	energy					1276:1281	a given collision energy	1258:1281	a given collision energy	1258:1281	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	8	9	theme	desired	1785:1791	arg1	range					1793:1797	a desired range	1783:1797	a desired range of fragmentation pathways	1783:1823	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	1	10	theme	spectrometry	210:221	arg1	capabilities					182:193	the capabilities	178:193	the capabilities of tandem mass spectrometry (MS/MS)	178:229	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	3	11	gly	glycopeptide	801:812	arg2	glycopeptide					801:812	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	3	12	theme	high	774:777	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	6	13	theme	given	1260:1264	arg1	energy					1276:1281	a given collision energy	1258:1281	a given collision energy	1258:1281	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	8	14	from	hinge	1846:1850	arg1	nature					1859:1864	the nature	1855:1864	the nature of the glycan	1855:1878	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	6	15	theme	structural	1350:1359	arg1	information					1361:1371	structural information	1350:1371	structural information	1350:1371	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	8	16	theme	pathways	1816:1823	arg1	range					1793:1797	a desired range	1783:1797	a desired range of fragmentation pathways	1783:1823	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	4	17	theme	%	932:932	arg1	energies					957:964	50% precursor ion survival energies	930:964	50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied	930:1052	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	2	18	theme	little	609:614	arg1	study					616:620	relatively little study	598:620	relatively little study	598:620	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	3	19	theme	glycopeptide	801:812	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	6	20	theme	depletion	1336:1344	arg1	information					1361:1371	structural information	1350:1371	structural information	1350:1371	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	6	20	theme	depletion	1336:1344	arg1	levels					1312:1317	highly similar levels	1297:1317	highly similar levels of precursor ion depletion	1297:1344	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	4	21	theme	ion	944:946	arg1	energies					957:964	50% precursor ion survival energies	930:964	50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied	930:1052	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	6	22	theme	this	1244:1247	arg1	consequence					1229:1239	The practical consequence	1215:1239	The practical consequence of this	1215:1247	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	6	23	theme	precursor	1322:1330	arg1	depletion					1336:1344	precursor ion depletion	1322:1344	precursor ion depletion	1322:1344	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	7	24	theme	energy-resolved	1650:1664	arg1	characteristics					1670:1684	their energy-resolved CID characteristics	1644:1684	their energy-resolved CID characteristics	1644:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	2	25	theme	carbohydrate	518:529	arg1	moiety					531:536	the carbohydrate moiety	514:536	the carbohydrate moiety	514:536	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	1	26	theme	fragmentation	367:379	arg1	pathways					381:388	their fragmentation pathways	361:388	their fragmentation pathways	361:388	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	3	27	theme	glycan	643:648	arg1	composition					650:660	glycan composition	643:660	glycan composition	643:660	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	3	28	link	N-linked	792:799	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	0	29	theme	dependencies	77:88	arg1	energies					23:30	Precursor ion survival energies	0:30	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.	0:160	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	2	30	theme	preferred	553:561	arg1	channels					576:583	the preferred dissociation channels	549:583	the preferred dissociation channels	549:583	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	2	31	from	characteristic	469:482	arg1	context					399:405	this context	394:405	this context	394:405	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	0	32	theme	high	93:96	arg1	composition					115:125	high mannose N-glycan composition	93:125	high mannose N-glycan composition in collision-induced dissociation	93:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	5	33	theme	ion	1117:1119	arg1	energies					1130:1137	the precursor ion survival energies	1103:1137	the precursor ion survival energies	1103:1137	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	8	34	theme	glycan	1873:1878	arg1	nature					1859:1864	the nature	1855:1864	the nature of the glycan	1855:1878	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	0	35	theme	N-glycan	106:113	arg1	composition					115:125	high mannose N-glycan composition	93:125	high mannose N-glycan composition in collision-induced dissociation	93:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	0	36	theme	Precursor	0:8	arg1	energies					23:30	Precursor ion survival energies	0:30	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.	0:160	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	2	37	theme	key	465:467	arg1	characteristic					469:482	a key characteristic	463:482	a key characteristic	463:482	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	2	37	theme	key	465:467	arg1	composition					420:430	the overall composition	408:430	the overall composition of a given glycopeptide ion	408:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	0	38	theme	survival	14:21	arg1	energies					23:30	Precursor ion survival energies	0:30	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.	0:160	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	1	39	theme	unimolecular	311:322	arg1	chemistry					337:345	the unimolecular dissociation chemistry	307:345	the unimolecular dissociation chemistry that dictates their fragmentation pathways	307:388	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	7	40	gly	glycopeptide	1540:1551	arg2	glycopeptide					1540:1551	glycopeptide	1540:1551	glycopeptide	1540:1551	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	4	41	theme	oligosaccharide	1027:1041	arg1	varied					1047:1052	varied	1047:1052	varied	1047:1052	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	4	41	theme	oligosaccharide	1027:1041	arg1	composition					1008:1018	composition	1008:1018	composition	1008:1018	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	4	41	theme	oligosaccharide	1027:1041	arg1	size					999:1002	size	999:1002	size	999:1002	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	4	42	theme	common	854:859	arg1	state					868:872	a common charge state	852:872	a common charge state	852:872	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	3	43	from	effect	633:638	arg1	behavior					718:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	2	44	theme	glycopeptide	443:454	arg1	ion					456:458	a given glycopeptide ion	435:458	a given glycopeptide ion	435:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	1	45	theme	chemistry	337:345	arg1	understanding					290:302	further understanding	282:302	further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways	282:388	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	3	46	theme	collision-induced	681:697	arg1	dissociation					699:710	collision-induced dissociation	681:710	energy-resolved collision-induced dissociation (CID) behavior	665:725	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	3	46	theme	collision-induced	681:697	arg1	CID					713:715	CID	713:715	CID	713:715	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	0	47	theme	N-glycopeptides	46:60	arg1	energies					23:30	Precursor ion survival energies	0:30	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.	0:160	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	5	48	theme	vibrational	1183:1193	arg1	degrees					1195:1201	available vibrational degrees	1173:1201	available vibrational degrees of freedom	1173:1212	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	7	49	theme	polypeptide	1565:1575	arg1	composition					1577:1587	polypeptide composition	1565:1587	polypeptide composition	1565:1587	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	7	49	theme	polypeptide	1565:1575	arg1	state					1597:1601	charge state	1590:1601	charge state	1590:1601	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	7	49	theme	polypeptide	1565:1575	arg1	carrier					1611:1617	charge carrier	1604:1617	charge carrier	1604:1617	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	4	50	theme	analytes	830:837	arg1	analytes					830:837	analytes	830:837	analytes which shared a common charge state, polypeptide sequence, and glycosylation site	830:918	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	4	50	theme	analytes	830:837	arg1	Groups					820:825	Groups	820:825	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site	820:918	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	4	51	gly	glycosylation	901:913	arg2	site					915:918	glycosylation site	901:918	glycosylation site	901:918	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	1	52	theme	glycosylated	247:258	arg1	peptides					260:267	glycosylated peptides	247:267	glycosylated peptides	247:267	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	7	53	theme	ions	1553:1556	arg1	properties					1526:1535	other physicochemical properties	1504:1535	other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics	1504:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	3	54	theme	select	745:750	arg1	menu					752:755	a select menu	743:755	a select menu of 30 protonated high mannose type N-linked glycopeptide ions	743:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	8	55	theme	deliberate	1731:1740	arg1	selection					1742:1750	the deliberate selection	1727:1750	the deliberate selection of CID energies to bring about a desired range of fragmentation pathways	1727:1823	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	6	56	theme	similar	1304:1310	arg1	levels					1312:1317	highly similar levels	1297:1317	highly similar levels of precursor ion depletion	1297:1344	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	1	57	theme	further	282:288	arg1	understanding					290:302	further understanding	282:302	further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways	282:388	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	7	58	theme	physicochemical	1510:1524	arg1	properties					1526:1535	other physicochemical properties	1504:1535	other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics	1504:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	3	59	theme	protonated	763:772	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	8	60	theme	energies	1759:1766	arg1	selection					1742:1750	the deliberate selection	1727:1750	the deliberate selection of CID energies to bring about a desired range of fragmentation pathways	1727:1823	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	6	61	theme	identity	1416:1423	arg1	variation					1392:1400	systematic variation	1381:1400	systematic variation of the glycan identity	1381:1423	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	1	62	theme	mass	205:208	arg1	MS/MS					224:228	MS/MS	224:228	MS/MS	224:228	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	1	62	theme	mass	205:208	arg1	spectrometry					210:221	tandem mass spectrometry	198:221	tandem mass spectrometry (MS/MS)	198:229	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	7	63	theme	oligosaccharide	1454:1468	arg1	composition					1470:1480	oligosaccharide composition	1454:1480	oligosaccharide composition	1454:1480	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	3	64	theme	N-linked	792:799	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	6	65	theme	systematic	1381:1390	arg1	variation					1392:1400	systematic variation	1381:1400	systematic variation of the glycan identity	1381:1423	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	4	66	theme	survival	948:955	arg1	energies					957:964	50% precursor ion survival energies	930:964	50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied	930:1052	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	7	67	theme	sensitivity	1439:1449	arg1	lack					1431:1434	This lack	1426:1434	This lack of sensitivity to oligosaccharide composition	1426:1480	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	3	68	theme	mannose	779:785	arg1	ions					814:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	30 protonated high mannose type N-linked glycopeptide ions	760:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	2	69	theme	dissociation	563:574	arg1	channels					576:583	the preferred dissociation channels	549:583	the preferred dissociation channels	549:583	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	7	70	dep	properties	1526:1535	arg1	contrast					1492:1499	contrast	1492:1499	contrast	1492:1499	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	0	71	theme	mannose	98:104	arg1	composition					115:125	high mannose N-glycan composition	93:125	high mannose N-glycan composition in collision-induced dissociation	93:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	5	72	theme	precursor	1107:1115	arg1	energies					1130:1137	the precursor ion survival energies	1103:1137	the precursor ion survival energies	1103:1137	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	8	73	theme	fragmentation	1802:1814	arg1	pathways					1816:1823	fragmentation pathways	1802:1823	fragmentation pathways	1802:1823	On the whole, these findings imply that the deliberate selection of CID energies to bring about a desired range of fragmentation pathways does not necessarily hinge on the nature of the glycan.					
30151520	0	74	theme	collision-induced	130:146	arg1	dissociation					148:159	collision-induced dissociation	130:159	collision-induced dissociation	130:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	7	75	theme	CID	1666:1668	arg1	characteristics					1670:1684	their energy-resolved CID characteristics	1644:1684	their energy-resolved CID characteristics	1644:1684	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	4	76	theme	50	930:931	arg1	%					932:932	%	932:932	%	932:932	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	0	77	from	composition	115:125	arg1	dissociation					148:159	collision-induced dissociation	130:159	collision-induced dissociation	130:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	3	78	theme	ions	814:817	arg1	menu					752:755	a select menu	743:755	a select menu of 30 protonated high mannose type N-linked glycopeptide ions	743:817	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	6	79	theme	ion	1332:1334	arg1	depletion					1336:1344	precursor ion depletion	1322:1344	precursor ion depletion	1322:1344	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	3	80	theme	composition	650:660	arg1	effect					633:638	the effect	629:638	the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior	629:725	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	4	81	theme	precursor	934:942	arg1	energies					957:964	50% precursor ion survival energies	930:964	50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied	930:1052	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	0	82	theme	weak	72:75	arg1	dependencies					77:88	their weak dependencies	66:88	their weak dependencies	66:88	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	0	83	from	energies	23:30	arg1	composition					115:125	high mannose N-glycan composition	93:125	high mannose N-glycan composition in collision-induced dissociation	93:159	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	6	84	theme	practical	1219:1227	arg1	consequence					1229:1239	The practical consequence	1215:1239	The practical consequence of this	1215:1247	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	6	85	theme	glycan	1409:1414	arg1	identity					1416:1423	the glycan identity	1405:1423	the glycan identity	1405:1423	The practical consequence of this was that a given collision energy brought about highly similar levels of precursor ion depletion and structural information despite systematic variation of the glycan identity.					
30151520	7	86	theme	charge	1604:1609	arg1	composition					1577:1587	polypeptide composition	1565:1587	polypeptide composition	1565:1587	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	7	86	theme	charge	1604:1609	arg1	carrier					1611:1617	charge carrier	1604:1617	charge carrier	1604:1617	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	2	87	gly	glycopeptide	443:454	arg2	glycopeptide					443:454	a given glycopeptide ion	435:458	a given glycopeptide ion	435:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	5	88	theme	survival	1121:1128	arg1	energies					1130:1137	the precursor ion survival energies	1103:1137	the precursor ion survival energies	1103:1137	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	1	89	gly	glycosylated	247:258	arg1	peptides					260:267	glycosylated peptides	247:267	glycosylated peptides	247:267	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	0	90	theme	ion	10:12	arg1	energies					23:30	Precursor ion survival energies	0:30	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.	0:160	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	2	91	theme	overall	412:418	arg1	characteristic					469:482	a key characteristic	463:482	a key characteristic	463:482	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	2	91	theme	overall	412:418	arg1	composition					420:430	the overall composition	408:430	the overall composition of a given glycopeptide ion	408:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	5	92	theme	freedom	1206:1212	arg1	degrees					1195:1201	available vibrational degrees	1173:1201	available vibrational degrees of freedom	1173:1212	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	3	93	theme	dissociation	699:710	arg1	behavior					718:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	2	94	theme	ion	456:458	arg1	characteristic					469:482	a key characteristic	463:482	a key characteristic	463:482	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	2	94	theme	ion	456:458	arg1	composition					420:430	the overall composition	408:430	the overall composition of a given glycopeptide ion	408:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	0	95	theme	protonated	35:44	arg1	N-glycopeptides					46:60	protonated N-glycopeptides	35:60	protonated N-glycopeptides	35:60	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	1	96	theme	dissociation	324:335	arg1	chemistry					337:345	the unimolecular dissociation chemistry	307:345	the unimolecular dissociation chemistry that dictates their fragmentation pathways	307:388	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30151520	3	97	theme	energy-resolved	665:679	arg1	behavior					718:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	energy-resolved collision-induced dissociation (CID) behavior	665:725	Here, the effect of glycan composition on energy-resolved collision-induced dissociation (CID) behavior was studied for a select menu of 30 protonated high mannose type N-linked glycopeptide ions.					
30151520	2	98	theme	given	437:441	arg1	ion					456:458	a given glycopeptide ion	435:458	a given glycopeptide ion	435:458	In this context, the overall composition of a given glycopeptide ion is a key characteristic; however, the extent to which the carbohydrate moiety influences the preferred dissociation channels has received relatively little study.					
30151520	5	99	theme	available	1173:1181	arg1	degrees					1195:1201	available vibrational degrees	1173:1201	available vibrational degrees of freedom	1173:1212	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	7	100	theme	charge	1590:1595	arg1	composition					1577:1587	polypeptide composition	1565:1587	polypeptide composition	1565:1587	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	7	100	theme	charge	1590:1595	arg1	state					1597:1601	charge state	1590:1601	charge state	1590:1601	This lack of sensitivity to oligosaccharide composition stands in contrast to other physicochemical properties of glycopeptide ions (e.g., polypeptide composition, charge state, charge carrier) which sharply influence their energy-resolved CID characteristics.					
30151520	4	101	theme	charge	861:866	arg1	state					868:872	a common charge state	852:872	a common charge state	852:872	Groups of analytes which shared a common charge state, polypeptide sequence, and glycosylation site exhibited 50% precursor ion survival energies that varied only slightly as the size and composition of the oligosaccharide was varied.					
30151520	5	102	theme	degrees	1195:1201	arg1	number					1163:1168	the number	1159:1168	the number of available vibrational degrees of freedom	1159:1212	This was found to be true regardless of whether the precursor ion survival energies were normalized for the number of available vibrational degrees of freedom.					
30151520	0	103	gly	N-glycopeptides	46:60	arg2	N-glycopeptides					46:60	protonated N-glycopeptides	35:60	protonated N-glycopeptides	35:60	Precursor ion survival energies of protonated N-glycopeptides and their weak dependencies on high mannose N-glycan composition in collision-induced dissociation.					
30151520	1	104	theme	peptides	260:267	arg1	analysis					235:242	analysis	235:242	analysis of glycosylated peptides	235:267	Fully realizing the capabilities of tandem mass spectrometry (MS/MS) for analysis of glycosylated peptides will require further understanding of the unimolecular dissociation chemistry that dictates their fragmentation pathways.					
30307368	4	0	theme	Fab-MICA	988:995	arg1	*					996:996	13A9 Fab-MICA*008	983:999	13A9 Fab-MICA*008	983:999	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	0	theme	Fab-MICA	988:995	arg1	6DDR					977:980	6DDR	977:980	6DDR (13A9 Fab-MICA*008)	977:1000	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	5	1	theme	shedding	1080:1087	arg1	inhibition					1061:1070	potent inhibition	1054:1070	potent inhibition of MICA shedding	1054:1087	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	2	2	from	tumors	442:447	arg1	shedding					428:435	MICA/B shedding	421:435	MICA/B shedding from tumors	421:447	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	5	3	theme	anti-MICA/B	1205:1215	arg1	antibodies					1217:1226	these anti-MICA/B antibodies	1199:1226	these anti-MICA/B antibodies	1199:1226	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	0	4	theme	inhibition	100:109	arg1	activity					111:118	inhibition activity	100:118	inhibition activity of anti-MICA antibodies	100:142	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	1	5	theme	natural	350:356	arg1	killer					358:363	natural killer	350:363	natural killer	350:363	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	6	theme	X-ray	893:897	arg1	crystallography					899:913	X-ray crystallography	893:913	X-ray crystallography	893:913	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	0	7	theme	anti-MICA	123:131	arg1	antibodies					133:142	anti-MICA antibodies	123:142	anti-MICA antibodies	123:142	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	1	8	theme	killer	358:363	arg1	responses					386:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	9	theme	Protein	916:922	arg1	Bank					929:932	Protein Data Bank	916:932	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	3	10	theme	glycosylation-engineered	574:597	arg1	GEM					616:618	GEM	616:618	GEM	616:618	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	3	10	theme	glycosylation-engineered	574:597	arg1	mapping					607:613	glycosylation-engineered epitope mapping	574:613	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	4	11	theme	important	778:786	arg1	epitopes					769:776	epitopes	769:776	epitopes important for shedding inhibition of MICA/B	769:820	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	5	12	theme	reported	1165:1172	arg1	sites					1183:1187	previously reported cleavage sites	1154:1187	previously reported cleavage sites	1154:1187	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	1	13	theme	cell-mediated	365:377	arg1	responses					386:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	3	14	theme	epitope	599:605	arg1	GEM					616:618	GEM	616:618	GEM	616:618	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	3	14	theme	epitope	599:605	arg1	mapping					607:613	glycosylation-engineered epitope mapping	574:613	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	5	15	theme	cleavage	1174:1181	arg1	sites					1183:1187	previously reported cleavage sites	1154:1187	previously reported cleavage sites	1154:1187	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	1	16	theme	immune	379:384	arg1	responses					386:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	17	theme	alanine	872:878	arg1	scanning					880:887	alanine scanning	872:887	alanine scanning	872:887	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	18	theme	MICA/B	815:820	arg1	inhibition					801:810	inhibition	801:810	inhibition of MICA/B	801:820	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	2	19	theme	MICA/B	421:426	arg1	shedding					428:435	MICA/B shedding	421:435	MICA/B shedding from tumors	421:447	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	0	20	theme	antibodies	133:142	arg1	activity					111:118	inhibition activity	100:118	inhibition activity of anti-MICA antibodies	100:142	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	1	21	theme	responses	386:394	arg1	impairment					318:327	impairment	318:327	impairment of CD8 + T cell- and natural killer cell-mediated immune responses	318:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	5	22	theme	MICA	1075:1078	arg1	shedding					1080:1087	MICA shedding	1075:1087	MICA shedding	1075:1087	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	0	23	theme	glycosylation	16:28	arg1	method					47:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	4	24	theme	13A9	983:986	arg1	*					996:996	13A9 Fab-MICA*008	983:999	13A9 Fab-MICA*008	983:999	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	24	theme	13A9	983:986	arg1	6DDR					977:980	6DDR	977:980	6DDR (13A9 Fab-MICA*008)	977:1000	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	25	theme	epitopes	769:776	arg1	discovery					756:764	the discovery	752:764	the discovery of epitopes important for shedding inhibition of MICA/B	752:820	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	5	26	theme	potent	1054:1059	arg1	inhibition					1061:1070	potent inhibition	1054:1070	potent inhibition of MICA shedding	1054:1087	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	0	27	theme	High-resolution	0:14	arg1	method					47:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	1	28	theme	major	195:199	arg1	class					228:232	major histocompatibility complex class I	195:234	the major histocompatibility complex class I homologs	191:243	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	29	theme	Fab-MICA	1013:1020	arg1	*					1021:1021	6E1 Fab-MICA*008	1009:1024	6E1 Fab-MICA*008	1009:1024	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	29	theme	Fab-MICA	1013:1020	arg1	6DDV					1003:1006	6DDV	1003:1006	6DDV (6E1 Fab-MICA*008)	1003:1025	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	1	30	theme	cancer	294:299	arg1	cells					301:305	cancer cells	294:305	cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses	294:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	3	31	theme	mapping	607:613	arg1	method					621:626	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	1	32	theme	histocompatibility	201:218	arg1	class					228:232	major histocompatibility complex class I	195:234	the major histocompatibility complex class I homologs	191:243	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	0	33	theme	site-engineering	30:45	arg1	method					47:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method	0:52	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	1	34	theme	cells	301:305	arg1	surface					283:289	the surface	279:289	the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses	279:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	3	35	dep	high-resolution	541:555	arg1	high-throughput					558:572	high-throughput	558:572	high-throughput	558:572	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	1	36	theme	complex	220:226	arg1	class					228:232	major histocompatibility complex class I	195:234	the major histocompatibility complex class I homologs	191:243	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	0	37	theme	MICA	65:68	arg1	epitope					70:76	MICA epitope	65:76	MICA epitope critical for shedding inhibition activity of anti-MICA antibodies	65:142	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	5	38	theme	adjacent	1142:1149	arg1	epitopes					1133:1140	GEM epitopes	1129:1140	GEM epitopes adjacent to previously reported cleavage sites	1129:1187	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	4	39	theme	Fab-MICA	962:969	arg1	*					970:970	1D5 Fab-MICA*008	958:973	1D5 Fab-MICA*008	958:973	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	39	theme	Fab-MICA	962:969	arg1	6DDM					952:955	6DDM	952:955	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	3	40	theme	antigen	697:703	arg1	surface					705:711	the antigen surface	693:711	the antigen surface	693:711	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	1	41	theme	class	228:232	arg1	homologs					236:243	the major histocompatibility complex class I homologs	191:243	the major histocompatibility complex class I homologs	191:243	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	1	41	theme	class	228:232	arg1	MICB					185:188	MICB	185:188	MICB	185:188	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	42	theme	1D5	958:960	arg1	*					970:970	1D5 Fab-MICA*008	958:973	1D5 Fab-MICA*008	958:973	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	42	theme	1D5	958:960	arg1	6DDM					952:955	6DDM	952:955	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	3	43	theme	glycans	680:686	arg1	insertion					658:666	site-specific insertion	644:666	site-specific insertion of N-linked glycans onto the antigen surface	644:711	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	0	44	theme	critical	78:85	arg1	epitope					70:76	MICA epitope	65:76	MICA epitope critical for shedding inhibition activity of anti-MICA antibodies	65:142	High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies.					
30307368	3	45	link	N-linked	671:678	arg1	glycans					680:686	N-linked glycans	671:686	N-linked glycans	671:686	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	4	46	theme	Bank	929:932	arg1	numbers					944:950	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	5	47	theme	GEM	1129:1131	arg1	epitopes					1133:1140	GEM epitopes	1129:1140	GEM epitopes adjacent to previously reported cleavage sites	1129:1187	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	4	48	theme	Data	924:927	arg1	Bank					929:932	Protein Data Bank	916:932	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	49	theme	residue	855:861	arg1	level					863:867	the residue level	851:867	the residue level	851:867	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	1	50	theme	CD8 + T	332:338	arg1	cell-					340:344	CD8 + T cell-	332:344	CD8 + T cell-	332:344	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	3	51	theme	local	721:725	arg1	regions					727:733	local regions	721:733	local regions	721:733	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	1	52	theme	immune	151:156	arg1	MICB					185:188	MICB	185:188	MICB	185:188	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	1	52	theme	immune	151:156	arg1	MICA					176:179	MICA	176:179	MICA	176:179	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	1	52	theme	immune	151:156	arg1	strategy					166:173	an immune evasion strategy	148:173	an immune evasion strategy	148:173	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	5	53	theme	tumor	1240:1244	arg1	growth					1246:1251	tumor growth	1240:1251	tumor growth	1240:1251	Furthermore, we show that potent inhibition of MICA shedding can be achieved by antibodies that bind GEM epitopes adjacent to previously reported cleavage sites, and that these anti-MICA/B antibodies can prevent tumor growth in vivo.					
30307368	4	54	theme	accession	934:942	arg1	numbers					944:950	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	3	55	theme	N-linked	671:678	arg1	glycans					680:686	N-linked glycans	671:686	N-linked glycans	671:686	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	1	56	theme	evasion	158:164	arg1	MICB					185:188	MICB	185:188	MICB	185:188	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	1	56	theme	evasion	158:164	arg1	MICA					176:179	MICA	176:179	MICA	176:179	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	1	56	theme	evasion	158:164	arg1	strategy					166:173	an immune evasion strategy	148:173	an immune evasion strategy	148:173	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	4	57	theme	6E1	1009:1011	arg1	*					1021:1021	6E1 Fab-MICA*008	1009:1024	6E1 Fab-MICA*008	1009:1024	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	57	theme	6E1	1009:1011	arg1	6DDV					1003:1006	6DDV	1003:1006	6DDV (6E1 Fab-MICA*008)	1003:1025	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	58	dep	numbers	944:950	arg1	*					970:970	1D5 Fab-MICA*008	958:973	1D5 Fab-MICA*008	958:973	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	4	58	dep	numbers	944:950	arg1	6DDM					952:955	6DDM	952:955	Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008)	916:974	We apply GEM to the discovery of epitopes important for shedding inhibition of MICA/B and validate the epitopes at the residue level by alanine scanning and X-ray crystallography (Protein Data Bank accession numbers 6DDM (1D5 Fab-MICA*008), 6DDR (13A9 Fab-MICA*008), 6DDV (6E1 Fab-MICA*008).					
30307368	2	59	contain	have	449:452	arg1	Antibodies					397:406	Antibodies	397:406	Antibodies that inhibit MICA/B shedding from tumors	397:447	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	2	59	contain	have	449:452	arg2	potential					466:474	therapeutic potential	454:474	therapeutic potential	454:474	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	3	60	theme	site-specific	644:656	arg1	insertion					658:666	site-specific insertion	644:666	site-specific insertion of N-linked glycans onto the antigen surface	644:711	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
30307368	2	61	theme	optimal	485:491	arg1	unknown					506:512	unknown	506:512	unknown	506:512	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	2	61	theme	optimal	485:491	arg1	epitopes					493:500	the optimal epitopes	481:500	the optimal epitopes	481:500	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	1	62	theme	cell-	340:344	arg1	responses					386:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	CD8 + T cell- and natural killer cell-mediated immune responses	332:394	As an immune evasion strategy, MICA and MICB, the major histocompatibility complex class I homologs, are proteolytically cleaved from the surface of cancer cells leading to impairment of CD8 + T cell- and natural killer cell-mediated immune responses.					
30307368	2	63	theme	therapeutic	454:464	arg1	potential					466:474	therapeutic potential	454:474	therapeutic potential	454:474	Antibodies that inhibit MICA/B shedding from tumors have therapeutic potential, but the optimal epitopes are unknown.					
30307368	3	64	theme	high-resolution	541:555	arg1	method					621:626	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method	539:626	Therefore, we developed a high-resolution, high-throughput glycosylation-engineered epitope mapping (GEM) method, which utilizes site-specific insertion of N-linked glycans onto the antigen surface to mask local regions.					
31062865	2	0	theme	media	639:643	arg1	effects					605:611	the effects	601:611	the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein	601:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	3	1	theme	O-glycopeptide	919:932	arg1	profiling					934:942	O-glycopeptide profiling	919:942	O-glycopeptide profiling	919:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	2	2	theme	analysis	373:380	arg1	method					382:387	an intact glycopeptide analysis method	350:387	an intact glycopeptide analysis method	350:387	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	6	3	theme	EPO	1438:1440	arg1	N-sites					1442:1448	the three EPO N-sites	1428:1448	the three EPO N-sites	1428:1448	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	10	4	theme	quality	2214:2220	arg1	media					2241:2245	media	2241:2245	media on recombinant proteins of biotechnology interest	2241:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	10	4	theme	quality	2214:2220	arg1	attributes					2222:2231	the key product quality attributes	2198:2231	the key product quality attributes such as media on recombinant proteins of biotechnology interest	2198:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	2	5	theme	commercial	620:629	arg1	media					639:643	two commercial culture media	616:643	two commercial culture media	616:643	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	5	theme	commercial	620:629	arg1	media					688:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	6	theme	intact	2019:2024	arg1	method					2039:2044	This intact glycopeptide method	2014:2044	This intact glycopeptide method	2014:2044	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	8	7	theme	sampling	1821:1828	arg1	time					1830:1833	the later sampling time	1811:1833	the later sampling time in both EX and IA media	1811:1857	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	6	8	theme	N-glycans	1522:1530	arg1	fraction					1488:1495	a greater fraction	1478:1495	a greater fraction of bi- and tri-antennary N-glycans at these same sites	1478:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	10	9	theme	biotechnology	2274:2286	arg1	interest					2288:2295	biotechnology interest	2274:2295	biotechnology interest	2274:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	10	10	theme	glycosylation	2073:2085	arg1	profile					2087:2093	site-specific glycosylation profile	2059:2093	site-specific glycosylation profile	2059:2093	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	2	11	theme	erythropoietin	477:490	arg1	protein					504:510	the recombinant erythropoietin Fc (EPO-Fc) protein	461:510	the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line	461:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	12	dep	EX-CELL	646:652	arg1	EX					655:656	EX	655:656	EX	655:656	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	13	theme	EX-CELL	646:652	arg1	media					639:643	two commercial culture media	616:643	two commercial culture media	616:643	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	13	theme	EX-CELL	646:652	arg1	media					688:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	14	theme	protein	738:744	arg1	profile					716:722	the glycosylation profile	698:722	the glycosylation profile of the target protein	698:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	7	15	from	site	1713:1716	arg1	maximum					1685:1691	a maximum	1683:1691	a maximum at the final IgG Fc site	1683:1716	Interestingly, the sialylation content decreased from sites 1-4 in both media while the fucosylation progressively increased with a maximum at the final IgG Fc site.					
31062865	0	16	theme	protein	125:131	arg1	glycosylation					94:106	site-specific glycosylation	80:106	site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells	80:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	6	17	theme	greater	1480:1486	arg1	fraction					1488:1495	a greater fraction	1478:1495	a greater fraction of bi- and tri-antennary N-glycans at these same sites	1478:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	1	18	theme	detailed	203:210	arg1	attributes					220:229	more detailed quality attributes	198:229	more detailed quality attributes	198:229	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	2	19	theme	cell	578:581	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	3	20	theme	Fc	770:771	arg1	region					773:778	the Fc region	766:778	the Fc region of immunoglobulin G1 (IgG1) fused to EPO	766:819	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	10	21	theme	interest	2288:2295	arg1	proteins					2262:2269	recombinant proteins	2250:2269	recombinant proteins of biotechnology interest	2250:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	9	22	gly	O-glycopeptides	1864:1878	arg2	O-glycopeptides					1864:1878	O-glycopeptides	1864:1878	O-glycopeptides	1864:1878	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	10	23	theme	detailed	2114:2121	arg1	characterization					2123:2138	a more detailed characterization	2107:2138	a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest	2107:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	5	24	theme	N-glycan	1250:1257	arg1	structures					1259:1268	tetra-branched N-glycan structures	1235:1268	tetra-branched N-glycan structures	1235:1268	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	3	25	theme	G1	798:799	arg1	region					773:778	the Fc region	766:778	the Fc region of immunoglobulin G1 (IgG1) fused to EPO	766:819	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	8	26	theme	low	1746:1748	arg1	Neu5Gc					1761:1766	Neu5Gc	1761:1766	Neu5Gc	1761:1766	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	8	26	theme	low	1746:1748	arg1	amounts					1750:1756	low amounts	1746:1756	low amounts of Neu5Gc	1746:1766	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	7	27	theme	IgG	1706:1708	arg1	site					1713:1716	the final IgG Fc site	1696:1716	the final IgG Fc site	1696:1716	Interestingly, the sialylation content decreased from sites 1-4 in both media while the fucosylation progressively increased with a maximum at the final IgG Fc site.					
31062865	2	28	theme	Chinese	528:534	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	29	theme	present	2160:2166	arg1	N-					2143:2144	N-	2143:2144	N-	2143:2144	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	0	30	theme	culture	63:69	arg1	media					71:75	culture media	63:75	culture media	63:75	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	1	31	theme	biopharmaceutical	298:314	arg1	manufacturing					316:328	biopharmaceutical manufacturing	298:328	biopharmaceutical manufacturing	298:328	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	2	32	theme	synthetase	560:569	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	33	theme	immediate	663:671	arg1	IA					684:685	IA	684:685	IA	684:685	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	33	theme	immediate	663:671	arg1	advantage					673:681	immediate advantage	663:681	immediate advantage (IA)	663:686	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	5	34	theme	intact	1059:1064	arg1	characterization					1079:1094	intact glycopeptide characterization	1059:1094	intact glycopeptide characterization	1059:1094	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	2	35	theme	ovary	544:548	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	0	36	from	impact	53:58	arg1	glycosylation					94:106	site-specific glycosylation	80:106	site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells	80:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	10	37	theme	enhanced	2172:2179	arg1	understanding					2181:2193	enhanced understanding	2172:2193	enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest	2172:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	0	38	theme	EPO-Fc	111:116	arg1	protein					125:131	EPO-Fc fusion protein	111:131	EPO-Fc fusion protein generated by CHO-GS cells	111:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	2	39	theme	protein	504:510	arg1	N-					433:434	the site-specific N- and O-glycan profiles	415:456	N-	433:434	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	39	theme	protein	504:510	arg1	profiles					449:456	the site-specific N- and O-glycan profiles	415:456	profiles	449:456	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	40	theme	Fc	492:493	arg1	protein					504:510	the recombinant erythropoietin Fc (EPO-Fc) protein	461:510	the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line	461:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	9	41	theme	lesser	1978:1983	arg1	amounts					1985:1991	lesser amounts	1978:1991	lesser amounts of other structures	1978:2011	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	9	41	theme	lesser	1978:1983	arg1	structures					2002:2011	other structures	1996:2011	other structures	1996:2011	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	2	42	theme	recombinant	465:475	arg1	protein					504:510	the recombinant erythropoietin Fc (EPO-Fc) protein	461:510	the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line	461:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	43	theme	key	2202:2204	arg1	media					2241:2245	media	2241:2245	media on recombinant proteins of biotechnology interest	2241:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	10	43	theme	key	2202:2204	arg1	attributes					2222:2231	the key product quality attributes	2198:2231	the key product quality attributes such as media on recombinant proteins of biotechnology interest	2198:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	0	44	theme	intact	20:25	arg1	glycopeptides					27:39	intact glycopeptides	20:39	intact glycopeptides	20:39	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	5	45	theme	Fc	1158:1159	arg1	characteristics					1170:1184	their individual EPO and Fc N-glycan characteristics	1133:1184	characteristics	1170:1184	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	6	46	from	fraction	1488:1495	arg1	sites					1546:1550	these same sites	1535:1550	these same sites	1535:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	3	47	theme	cell	862:865	arg1	culture					867:873	a batch cell culture	854:873	a batch cell culture process followed by purification and N- and O-glycopeptide profiling	854:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	4	48	theme	mixed	947:951	arg1	column					984:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	10	49	from	media	2241:2245	arg1	proteins					2262:2269	recombinant proteins	2250:2269	recombinant proteins of biotechnology interest	2250:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	9	50	theme	structures	2002:2011	arg1	amounts					1985:1991	lesser amounts	1978:1991	lesser amounts of other structures	1978:2011	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	9	50	theme	structures	2002:2011	arg1	structures					2002:2011	other structures	1996:2011	other structures	1996:2011	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	2	51	theme	O-glycan	440:447	arg1	profiles					449:456	the site-specific N- and O-glycan profiles	415:456	profiles	449:456	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	6	52	theme	tetra-antennary	1391:1405	arg1	N-glycans					1407:1415	more complex tetra-antennary N-glycans	1378:1415	more complex tetra-antennary N-glycans	1378:1415	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	4	53	theme	exchange	959:966	arg1	column					984:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	2	54	theme	glycopeptide	360:371	arg1	method					382:387	an intact glycopeptide analysis method	350:387	an intact glycopeptide analysis method	350:387	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	0	55	gly	glycopeptides	27:39	arg2	glycopeptides					27:39	intact glycopeptides	20:39	intact glycopeptides	20:39	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	5	56	theme	EPO	1199:1201	arg1	region					1203:1208	the EPO region	1195:1208	the EPO region	1195:1208	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	10	57	theme	site-specific	2059:2071	arg1	profile					2087:2093	site-specific glycosylation profile	2059:2093	site-specific glycosylation profile	2059:2093	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	6	58	theme	IA	1456:1457	arg1	medium					1459:1464	IA medium	1456:1464	IA medium	1456:1464	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	1	59	theme	sophisticated	237:249	arg1	tools					267:271	more sophisticated glycan analysis tools	232:271	more sophisticated glycan analysis tools	232:271	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	2	60	theme	EPO-Fc	496:501	arg1	protein					504:510	the recombinant erythropoietin Fc (EPO-Fc) protein	461:510	the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line	461:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	6	61	theme	EX	1359:1360	arg1	medium					1362:1367	EX medium	1359:1367	EX medium	1359:1367	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	8	62	theme	later	1815:1819	arg1	time					1830:1833	the later sampling time	1811:1833	the later sampling time in both EX and IA media	1811:1857	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	1	63	theme	analysis	258:265	arg1	tools					267:271	more sophisticated glycan analysis tools	232:271	more sophisticated glycan analysis tools	232:271	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	2	64	theme	culture	631:637	arg1	media					639:643	two commercial culture media	616:643	two commercial culture media	616:643	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	64	theme	culture	631:637	arg1	media					688:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	65	theme	glycopeptide	2026:2037	arg1	method					2039:2044	This intact glycopeptide method	2014:2044	This intact glycopeptide method	2014:2044	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	2	66	from	effects	605:611	arg1	profile					716:722	the glycosylation profile	698:722	the glycosylation profile of the target protein	698:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	4	67	link	N-linked	1029:1036	arg1	glycopeptides					1038:1050	N-linked glycopeptides	1029:1050	N-linked glycopeptides	1029:1050	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	6	68	theme	tri-antennary	1508:1520	arg1	N-glycans					1522:1530	tri-antennary N-glycans	1508:1530	tri-antennary N-glycans	1508:1530	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	1	69	theme	increasing	169:178	arg1	demand					180:185	the increasing demand	165:185	the increasing demand to provide more detailed quality attributes	165:229	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	3	70	gly	O-glycopeptide	919:932	arg2	O-glycopeptide					919:932	O-glycopeptide profiling	919:942	O-glycopeptide profiling	919:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	6	71	theme	bi-	1500:1502	arg1	fraction					1488:1495	a greater fraction	1478:1495	a greater fraction of bi- and tri-antennary N-glycans at these same sites	1478:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	10	72	theme	recombinant	2250:2260	arg1	proteins					2262:2269	recombinant proteins	2250:2269	recombinant proteins of biotechnology interest	2250:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	1	73	with	desirable	284:292	arg1	demand					180:185	the increasing demand	165:185	the increasing demand to provide more detailed quality attributes	165:229	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	5	74	gly	glycopeptide	1066:1077	arg2	glycopeptide					1066:1077	intact glycopeptide characterization	1059:1094	intact glycopeptide characterization	1059:1094	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	6	75	theme	same	1541:1544	arg1	sites					1546:1550	these same sites	1535:1550	these same sites	1535:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	0	76	theme	CHO-GS	146:151	arg1	cells					153:157	CHO-GS cells	146:157	CHO-GS cells	146:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	4	77	gly	glycopeptides	1038:1050	arg2	glycopeptides					1038:1050	N-linked glycopeptides	1029:1050	N-linked glycopeptides	1029:1050	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	1	78	theme	quality	212:218	arg1	attributes					220:229	more detailed quality attributes	198:229	more detailed quality attributes	198:229	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31062865	2	79	theme	stable	571:576	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	80	theme	glycosylation	702:714	arg1	profile					716:722	the glycosylation profile	698:722	the glycosylation profile of the target protein	698:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	7	81	theme	sialylation	1572:1582	arg1	content					1584:1590	the sialylation content	1568:1590	the sialylation content	1568:1590	Interestingly, the sialylation content decreased from sites 1-4 in both media while the fucosylation progressively increased with a maximum at the final IgG Fc site.					
31062865	5	82	attach	presented	1210:1218	arg2	region					1203:1208	the EPO region	1195:1208	the EPO region	1195:1208	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	5	82	attach	presented	1210:1218	arg1	characteristics					1170:1184	their individual EPO and Fc N-glycan characteristics	1133:1184	characteristics	1170:1184	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	5	82	attach	presented	1210:1218	arg1	EPO					1150:1152	their individual EPO and Fc N-glycan characteristics	1133:1184	EPO	1150:1152	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	5	83	theme	tetra-branched	1235:1248	arg1	structures					1259:1268	tetra-branched N-glycan structures	1235:1268	tetra-branched N-glycan structures	1235:1268	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	2	84	theme	target	731:736	arg1	protein					738:744	the target protein	727:744	the target protein	727:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	0	85	theme	media	71:75	arg1	impact					53:58	the impact	49:58	the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells	49:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	3	86	dep	culture	867:873	arg1	process					875:881	process	875:881	a batch cell culture process followed by purification and N- and O-glycopeptide profiling	854:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	7	87	theme	Fc	1710:1711	arg1	site					1713:1716	the final IgG Fc site	1696:1716	the final IgG Fc site	1696:1716	Interestingly, the sialylation content decreased from sites 1-4 in both media while the fucosylation progressively increased with a maximum at the final IgG Fc site.					
31062865	10	88	theme	O-glycans	2150:2158	arg1	characterization					2123:2138	a more detailed characterization	2107:2138	a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest	2107:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	3	89	theme	immunoglobulin	783:796	arg1	G1					798:799	immunoglobulin G1	783:799	immunoglobulin G1 (IgG1)	783:806	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	3	89	theme	immunoglobulin	783:796	arg1	IgG1					802:805	IgG1	802:805	IgG1	802:805	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	0	90	theme	site-specific	80:92	arg1	glycosylation					94:106	site-specific glycosylation	80:106	site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells	80:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	6	91	from	sites	1546:1550	arg1	fraction					1488:1495	a greater fraction	1478:1495	a greater fraction of bi- and tri-antennary N-glycans at these same sites	1478:1550	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	6	91	from	sites	1546:1550	arg1	bi-					1500:1502	bi-	1500:1502	bi-	1500:1502	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	6	91	from	sites	1546:1550	arg1	N-glycans					1522:1530	tri-antennary N-glycans	1508:1530	tri-antennary N-glycans	1508:1530	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	7	92	theme	final	1700:1704	arg1	site					1713:1716	the final IgG Fc site	1696:1716	the final IgG Fc site	1696:1716	Interestingly, the sialylation content decreased from sites 1-4 in both media while the fucosylation progressively increased with a maximum at the final IgG Fc site.					
31062865	8	93	theme	IA	1850:1851	arg1	media					1853:1857	IA media	1850:1857	IA media	1850:1857	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	2	94	theme	advantage	673:681	arg1	media					639:643	two commercial culture media	616:643	two commercial culture media	616:643	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	94	theme	advantage	673:681	arg1	media					688:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	EX-CELL (EX) and immediate advantage (IA) media	646:692	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	8	95	theme	Neu5Gc	1761:1766	arg1	Neu5Gc					1761:1766	Neu5Gc	1761:1766	Neu5Gc	1761:1766	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	8	95	theme	Neu5Gc	1761:1766	arg1	amounts					1750:1756	low amounts	1746:1756	low amounts of Neu5Gc	1746:1766	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	2	96	theme	glutamine	550:558	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	97	gly	glycopeptide	2026:2037	arg2	glycopeptide					2026:2037	This intact glycopeptide method	2014:2044	This intact glycopeptide method	2014:2044	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	3	98	contain	containing	755:764	arg1	EPO-Fc					747:752	EPO-Fc	747:752	EPO-Fc	747:752	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	3	98	contain	containing	755:764	arg2	region					773:778	the Fc region	766:778	the Fc region of immunoglobulin G1 (IgG1) fused to EPO	766:819	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	0	99	theme	fusion	118:123	arg1	protein					125:131	EPO-Fc fusion protein	111:131	EPO-Fc fusion protein generated by CHO-GS cells	111:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	5	100	theme	glycopeptide	1066:1077	arg1	characterization					1079:1094	intact glycopeptide characterization	1059:1094	intact glycopeptide characterization	1059:1094	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	3	101	theme	batch	856:860	arg1	culture					867:873	a batch cell culture	854:873	a batch cell culture process followed by purification and N- and O-glycopeptide profiling	854:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	2	102	theme	hamster	536:542	arg1	line					583:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	a Chinese hamster ovary glutamine synthetase stable cell line	526:586	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	10	103	theme	attributes	2222:2231	arg1	understanding					2181:2193	enhanced understanding	2172:2193	enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest	2172:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	5	104	theme	biantennary	1310:1320	arg1	glycans					1322:1328	biantennary glycans	1310:1328	biantennary glycans	1310:1328	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	6	105	theme	EPO-Fc	1331:1336	arg1	protein					1338:1344	EPO-Fc protein	1331:1344	EPO-Fc protein generated in EX medium	1331:1367	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	0	106	gly	glycosylation	94:106	arg1	protein					125:131	EPO-Fc fusion protein	111:131	EPO-Fc fusion protein generated by CHO-GS cells	111:157	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	4	107	theme	chromatographic	968:982	arg1	column					984:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	8	108	from	time	1830:1833	arg1	EX					1843:1844	EX	1843:1844	EX	1843:1844	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	8	108	from	time	1830:1833	arg1	media					1853:1857	IA media	1850:1857	IA media	1850:1857	Moreover, we observed that low amounts of Neu5Gc were detected and the content increased at the later sampling time in both EX and IA media.					
31062865	10	109	theme	product	2206:2212	arg1	media					2241:2245	media	2241:2245	media on recombinant proteins of biotechnology interest	2241:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	10	109	theme	product	2206:2212	arg1	attributes					2222:2231	the key product quality attributes	2198:2231	the key product quality attributes such as media on recombinant proteins of biotechnology interest	2198:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	0	110	theme	glycopeptides	27:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of intact glycopeptides	0:39	Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells.					
31062865	9	111	theme	other	1996:2000	arg1	structures					2002:2011	other structures	1996:2011	other structures	1996:2011	For O-glycopeptides, both media produced predominantly three structures, N1F1F0SOG0, N1H1F0S1G0, and N1H1F0S2G0, with lesser amounts of other structures.					
31062865	5	112	theme	Fc	1281:1282	arg1	N-glycan					1284:1291	the Fc N-glycan	1277:1291	the Fc N-glycan	1277:1291	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	5	113	theme	N-glycan	1161:1168	arg1	characteristics					1170:1184	their individual EPO and Fc N-glycan characteristics	1133:1184	characteristics	1170:1184	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	3	114	theme	culture	867:873	arg1	Day					839:841	Day 5 and 8	839:849	Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling	839:942	EPO-Fc, containing the Fc region of immunoglobulin G1 (IgG1) fused to EPO, was harvested at Day 5 and 8 of a batch cell culture process followed by purification and N- and O-glycopeptide profiling.					
31062865	6	115	theme	complex	1383:1389	arg1	N-glycans					1407:1415	more complex tetra-antennary N-glycans	1378:1415	more complex tetra-antennary N-glycans	1378:1415	EPO-Fc protein generated in EX medium produced more complex tetra-antennary N-glycans at each of the three EPO N-sites while IA medium resulted in a greater fraction of bi- and tri-antennary N-glycans at these same sites.					
31062865	2	116	gly	glycosylation	702:714	arg1	protein					738:744	the target protein	727:744	the target protein	727:744	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	4	117	theme	anion	953:957	arg1	column					984:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column	945:989	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	2	118	gly	glycopeptide	360:371	arg2	glycopeptide					360:371	an intact glycopeptide analysis method	350:387	an intact glycopeptide analysis method	350:387	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	2	119	theme	intact	353:358	arg1	method					382:387	an intact glycopeptide analysis method	350:387	an intact glycopeptide analysis method	350:387	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	5	120	theme	individual	1139:1148	arg1	EPO					1150:1152	their individual EPO and Fc N-glycan characteristics	1133:1184	EPO	1150:1152	Using intact glycopeptide characterization, the EPO-Fc was observed to maintain their individual EPO and Fc N-glycan characteristics in which the EPO region presented bi-, tri-, and tetra-branched N-glycan structures, while the Fc N-glycan displayed mostly biantennary glycans.					
31062865	4	121	theme	N-linked	1029:1036	arg1	glycopeptides					1038:1050	N-linked glycopeptides	1029:1050	N-linked glycopeptides	1029:1050	A mixed anion exchange chromatographic column was implemented to capture and enrich N-linked glycopeptides.					
31062865	10	122	theme	N-	2143:2144	arg1	characterization					2123:2138	a more detailed characterization	2107:2138	a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest	2107:2295	This intact glycopeptide method can decipher site-specific glycosylation profile and provide a more detailed characterization of N- and O-glycans present for enhanced understanding of the key product quality attributes such as media on recombinant proteins of biotechnology interest.					
31062865	2	123	theme	site-specific	419:431	arg1	N-					433:434	the site-specific N- and O-glycan profiles	415:456	N-	433:434	Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the recombinant erythropoietin Fc (EPO-Fc) protein secreted from a Chinese hamster ovary glutamine synthetase stable cell line and compared the effects of two commercial culture media, EX-CELL (EX) and immediate advantage (IA) media, on the glycosylation profile of the target protein.					
31062865	1	124	theme	glycan	251:256	arg1	tools					267:271	more sophisticated glycan analysis tools	232:271	more sophisticated glycan analysis tools	232:271	With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing.					
31443278	6	0	theme	LMA-based	1185:1193	arg1	atlas					1271:1275	an atlas	1268:1275	an atlas	1268:1275	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	6	0	theme	LMA-based	1185:1193	arg1	data					1221:1224	the LMA-based tissue glycomic profiling data	1181:1224	the LMA-based tissue glycomic profiling data associated with the sample information	1181:1263	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	4	1	theme	8-week-old	861:870	arg1	mice					886:889	two 8-week-old male C57BL/6J mice	857:889	two 8-week-old male C57BL/6J mice	857:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	2	2	theme	various	375:381	arg1	types					383:387	various types	375:387	various types of cells, sites, and tissues	375:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	6	3	theme	online	1116:1121	arg1	tool					1123:1126	This online tool	1111:1126	This online tool	1111:1126	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	2	4	from	roles	171:175	arg1	samples					253:259	tissue samples	246:259	tissue samples	246:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	5	5	theme	types	1096:1100	arg1	comparison					1074:1083	easy comparison	1069:1083	easy comparison of the two types of data	1069:1108	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	2	6	from	alterations	198:208	arg1	samples					253:259	tissue samples	246:259	tissue samples	246:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	1	7	theme	Mouse	102:106	arg1	Sections					115:122	Mouse Tissue Sections	102:122	Mouse Tissue Sections	102:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	3	8	theme	in-depth	565:572	arg1	profiling					583:591	high-throughput and in-depth glycomic profiling	545:591	profiling	583:591	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	7	9	theme	glycosylation	1411:1423	arg1	patterns					1425:1432	site- and tissue-specific glycosylation patterns	1385:1432	site- and tissue-specific glycosylation patterns	1385:1432	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	7	10	theme	site-	1385:1389	arg1	patterns					1425:1432	site- and tissue-specific glycosylation patterns	1385:1432	site- and tissue-specific glycosylation patterns	1385:1432	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	4	11	theme	glycomic	707:714	arg1	data					726:729	234 glycomic profiling data	703:729	234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	703:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	8	12	theme	deposited	1534:1542	arg1	data					1544:1547	deposited data	1534:1547	deposited data	1534:1547	Taking advantage of its extensibility, this tool will continue to be updated with the expansion of deposited data.					
31443278	3	13	theme	glycomic	574:581	arg1	profiling					583:591	high-throughput and in-depth glycomic profiling	545:591	profiling	583:591	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	5	14	theme	easy	1069:1072	arg1	comparison					1074:1083	easy comparison	1069:1083	easy comparison of the two types of data	1069:1108	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	6	15	theme	profiling	1211:1219	arg1	atlas					1271:1275	an atlas	1268:1275	an atlas	1268:1275	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	6	15	theme	profiling	1211:1219	arg1	data					1221:1224	the LMA-based tissue glycomic profiling data	1181:1224	the LMA-based tissue glycomic profiling data associated with the sample information	1181:1263	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	4	16	theme	tissue	750:755	arg1	thymus					790:795	thymus	790:795	thymus	790:795	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	intestine					826:834	small intestine	820:834	small intestine	820:834	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	pancreas					767:774	pancreas	767:774	pancreas	767:774	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	colon					837:841	colon	837:841	colon	837:841	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	heart					777:781	heart	777:781	heart	777:781	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	gallbladder					798:808	gallbladder	798:808	gallbladder	798:808	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	stomach					811:817	stomach	811:817	stomach	811:817	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	sections					757:764	nine tissue sections	745:764	nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	745:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	lung					784:787	lung	784:787	lung	784:787	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	16	theme	tissue	750:755	arg1	skin					848:851	skin	848:851	skin	848:851	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	1	17	theme	Lectin	57:62	arg1	Profiles					90:97	Lectin Microarray-Based Glycomic Profiles	57:97	Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections	57:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	7	18	theme	present	1288:1294	arg1	dataset					1296:1302	the present dataset	1284:1302	the present dataset	1284:1302	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	4	19	theme	"	680:680	arg1	approach					682:689	this "tissue glycome mapping" approach	652:689	this "tissue glycome mapping" approach	652:689	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	thymus					790:795	thymus	790:795	thymus	790:795	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	intestine					826:834	small intestine	820:834	small intestine	820:834	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	pancreas					767:774	pancreas	767:774	pancreas	767:774	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	colon					837:841	colon	837:841	colon	837:841	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	heart					777:781	heart	777:781	heart	777:781	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	gallbladder					798:808	gallbladder	798:808	gallbladder	798:808	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	stomach					811:817	stomach	811:817	stomach	811:817	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	sections					757:764	nine tissue sections	745:764	nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	745:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	lung					784:787	lung	784:787	lung	784:787	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	20	dep	sections	757:764	arg1	skin					848:851	skin	848:851	skin	848:851	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	2	21	theme	effective	133:141	arg1	discovery					143:151	the effective discovery	129:151	the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples	129:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	4	22	theme	small	820:824	arg1	sections					757:764	nine tissue sections	745:764	nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	745:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	22	theme	small	820:824	arg1	intestine					826:834	small intestine	820:834	small intestine	820:834	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	1	23	theme	Microarray-Based	64:79	arg1	Profiles					90:97	Lectin Microarray-Based Glycomic Profiles	57:97	Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections	57:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	2	24	from	samples	253:259	arg1	discovery					143:151	the effective discovery	129:151	the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples	129:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	1	25	dep	1.0	21:23	arg1	Tool					48:51	A Novel Visualization Tool	26:51	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.	21:123	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	4	26	theme	tissue	658:663	arg1	approach					682:689	this "tissue glycome mapping" approach	652:689	this "tissue glycome mapping" approach	652:689	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	6	27	theme	tissue	1195:1200	arg1	atlas					1271:1275	an atlas	1268:1275	an atlas	1268:1275	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	6	27	theme	tissue	1195:1200	arg1	data					1221:1224	the LMA-based tissue glycomic profiling data	1181:1224	the LMA-based tissue glycomic profiling data associated with the sample information	1181:1263	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	4	28	theme	mapping	673:679	arg1	approach					682:689	this "tissue glycome mapping" approach	652:689	this "tissue glycome mapping" approach	652:689	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	1	29	theme	Glycomic	81:88	arg1	Profiles					90:97	Lectin Microarray-Based Glycomic Profiles	57:97	Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections	57:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	7	30	theme	patterns	1425:1432	arg1	evaluation					1371:1380	the evaluation	1367:1380	the evaluation of site- and tissue-specific glycosylation patterns	1367:1432	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	4	31	theme	glycome	665:671	arg1	approach					682:689	this "tissue glycome mapping" approach	652:689	this "tissue glycome mapping" approach	652:689	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	5	32	theme	spectrometry-based	1014:1031	arg1	profiling					1049:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	2	33	theme	qualitative	318:328	arg1	variations					330:339	the quantitative and qualitative variations	297:339	the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues	297:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	2	34	theme	cells	392:396	arg1	types					383:387	various types	375:387	various types of cells, sites, and tissues	375:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	3	35	theme	tissue	629:634	arg1	sections					636:643	formalin-fixed paraffin-embedded tissue sections	596:643	formalin-fixed paraffin-embedded tissue sections	596:643	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	4	36	theme	mice	886:889	arg1	thymus					790:795	thymus	790:795	thymus	790:795	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	intestine					826:834	small intestine	820:834	small intestine	820:834	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	pancreas					767:774	pancreas	767:774	pancreas	767:774	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	colon					837:841	colon	837:841	colon	837:841	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	heart					777:781	heart	777:781	heart	777:781	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	gallbladder					798:808	gallbladder	798:808	gallbladder	798:808	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	stomach					811:817	stomach	811:817	stomach	811:817	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	sections					757:764	nine tissue sections	745:764	nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	745:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	lung					784:787	lung	784:787	lung	784:787	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	4	36	theme	mice	886:889	arg1	skin					848:851	skin	848:851	skin	848:851	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	3	37	theme	high-throughput	545:559	arg1	profiling					583:591	high-throughput and in-depth glycomic profiling	545:591	profiling	583:591	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	7	38	theme	glycomic	1329:1336	arg1	profiles					1338:1345	glycomic profiles	1329:1345	glycomic profiles	1329:1345	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	3	39	theme	microdissection-assisted	446:469	arg1	LMA					490:492	LMA	490:492	LMA	490:492	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	3	39	theme	microdissection-assisted	446:469	arg1	microarray					478:487	laser microdissection-assisted lectin microarray	440:487	laser microdissection-assisted lectin microarray (LMA)	440:493	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	3	40	used	used	435:438	arg2	we					432:433	we	432:433	we	432:433	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	4	41	theme	C57BL/6J	877:884	arg1	mice					886:889	two 8-week-old male C57BL/6J mice	857:889	two 8-week-old male C57BL/6J mice	857:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	5	42	theme	tissue	1033:1038	arg1	profiling					1049:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	2	43	theme	quantitative	301:312	arg1	variations					330:339	the quantitative and qualitative variations	297:339	the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues	297:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	3	44	theme	lectin	471:476	arg1	LMA					490:492	LMA	490:492	LMA	490:492	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	3	44	theme	lectin	471:476	arg1	microarray					478:487	laser microdissection-assisted lectin microarray	440:487	laser microdissection-assisted lectin microarray (LMA)	440:493	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	2	45	theme	structures	351:360	arg1	variations					330:339	the quantitative and qualitative variations	297:339	the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues	297:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	2	46	theme	roles	171:175	arg1	discovery					143:151	the effective discovery	129:151	the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples	129:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	3	47	theme	formalin-fixed	596:609	arg1	sections					636:643	formalin-fixed paraffin-embedded tissue sections	596:643	formalin-fixed paraffin-embedded tissue sections	596:643	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	6	48	theme	LM-GlycomeAtlas	1137:1151	arg1	"					1152:1152	LM-GlycomeAtlas"	1137:1152	LM-GlycomeAtlas"	1137:1152	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	2	49	theme	tissues	410:416	arg1	types					383:387	various types	375:387	various types of cells, sites, and tissues	375:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	6	50	theme	glycomic	1202:1209	arg1	atlas					1271:1275	an atlas	1268:1275	an atlas	1268:1275	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	6	50	theme	glycomic	1202:1209	arg1	data					1221:1224	the LMA-based tissue glycomic profiling data	1181:1224	the LMA-based tissue glycomic profiling data associated with the sample information	1181:1263	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	5	51	theme	data	1105:1108	arg1	types					1096:1100	the two types	1088:1100	the two types of data	1088:1108	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	2	52	theme	biological	160:169	arg1	roles					171:175	the biological roles	156:175	the biological roles	156:175	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	2	53	theme	tissue	246:251	arg1	samples					253:259	tissue samples	246:259	tissue samples	246:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	3	54	theme	laser	440:444	arg1	LMA					490:492	LMA	490:492	LMA	490:492	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	3	54	theme	laser	440:444	arg1	microarray					478:487	laser microdissection-assisted lectin microarray	440:487	laser microdissection-assisted lectin microarray (LMA)	440:493	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	7	55	theme	profiles	1338:1345	arg1	comparison					1315:1324	the comparison	1311:1324	the comparison of glycomic profiles	1311:1345	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	1	56	theme	Tissue	108:113	arg1	Sections					115:122	Mouse Tissue Sections	102:122	Mouse Tissue Sections	102:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	2	57	from	discovery	143:151	arg1	samples					253:259	tissue samples	246:259	tissue samples	246:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	8	58	theme	data	1544:1547	arg1	expansion					1521:1529	the expansion	1517:1529	the expansion of deposited data	1517:1547	Taking advantage of its extensibility, this tool will continue to be updated with the expansion of deposited data.					
31443278	2	59	theme	protein	221:227	arg1	glycosylation					229:241	protein glycosylation	221:241	protein glycosylation	221:241	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	5	60	theme	developed	990:998	arg1	tool					1000:1003	a previously developed tool	977:1003	a previously developed tool for mass spectrometry-based tissue glycomic profiling	977:1057	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	5	60	theme	developed	990:998	arg1	GlycomeAtlas					963:974	GlycomeAtlas	963:974	GlycomeAtlas	963:974	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	1	61	theme	Sections	115:122	arg1	Profiles					90:97	Lectin Microarray-Based Glycomic Profiles	57:97	Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections	57:122	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	3	62	theme	sections	636:643	arg1	profiling					583:591	high-throughput and in-depth glycomic profiling	545:591	profiling	583:591	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	4	63	theme	male	872:875	arg1	mice					886:889	two 8-week-old male C57BL/6J mice	857:889	two 8-week-old male C57BL/6J mice	857:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
31443278	3	64	theme	paraffin-embedded	611:627	arg1	sections					636:643	formalin-fixed paraffin-embedded tissue sections	596:643	formalin-fixed paraffin-embedded tissue sections	596:643	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	2	65	theme	glycan	344:349	arg1	structures					351:360	glycan structures	344:360	glycan structures expressed in various types of cells, sites, and tissues	344:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	5	66	theme	LMA-based	909:917	arg1	dataset					919:925	this LMA-based dataset	904:925	this LMA-based dataset	904:925	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	3	67	theme	reproducible	521:532	arg1	method					534:539	a simple and reproducible method	508:539	a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections	508:643	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	2	68	theme	alterations	198:208	arg1	discovery					143:151	the effective discovery	129:151	the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples	129:259	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	5	69	theme	similar	934:940	arg1	interface					942:950	the similar interface	930:950	the similar interface	930:950	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	5	70	theme	glycomic	1040:1047	arg1	profiling					1049:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	mass spectrometry-based tissue glycomic profiling	1009:1057	We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data.					
31443278	6	71	theme	sample	1246:1251	arg1	information					1253:1263	the sample information	1242:1263	the sample information	1242:1263	This online tool, called "LM-GlycomeAtlas", allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas.					
31443278	2	72	theme	disease-specific	181:196	arg1	alterations					198:208	disease-specific alterations	181:208	disease-specific alterations	181:208	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	1	73	theme	Novel	28:32	arg1	Tool					48:51	A Novel Visualization Tool	26:51	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.	21:123	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	2	74	dep	know	281:284	arg1	beforehand					286:295	beforehand	286:295	beforehand	286:295	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	2	75	theme	sites	399:403	arg1	types					383:387	various types	375:387	various types of cells, sites, and tissues	375:416	For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues.					
31443278	7	76	theme	tissue-specific	1395:1409	arg1	patterns					1425:1432	site- and tissue-specific glycosylation patterns	1385:1432	site- and tissue-specific glycosylation patterns	1385:1432	Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns.					
31443278	8	77	theme	extensibility	1459:1471	arg1	advantage					1442:1450	advantage	1442:1450	advantage of its extensibility	1442:1471	Taking advantage of its extensibility, this tool will continue to be updated with the expansion of deposited data.					
31443278	1	78	theme	Visualization	34:46	arg1	Tool					48:51	A Novel Visualization Tool	26:51	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.	21:123	1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.					
31443278	3	79	theme	simple	510:515	arg1	method					534:539	a simple and reproducible method	508:539	a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections	508:643	To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections.					
31443278	4	80	theme	profiling	716:724	arg1	data					726:729	234 glycomic profiling data	703:729	234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice	703:889	Using this "tissue glycome mapping" approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice.					
30503907	8	0	theme	animal-derived	1131:1144	arg1	virions					1146:1152	human- and animal-derived virions	1120:1152	human- and animal-derived virions	1120:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	8	1	theme	direct	1094:1099	arg1	analyses					1108:1115	direct glycan analyses	1094:1115	direct glycan analyses of human- and animal-derived virions	1094:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	4	2	theme	samples	586:592	arg1	1 ml					563:566	1 ml	563:566	1 ml of the 106 TCID50 samples	563:592	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	0	3	theme	hemagglutinins	76:89	arg1	profiles					60:67	host cell-specific glycan profiles	34:67	host cell-specific glycan profiles of the hemagglutinins of influenza A viruses	34:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	0	4	theme	A	104:104	arg1	viruses					106:112	influenza A viruses	94:112	influenza A viruses	94:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	4	5	theme	glycan	527:532	arg1	profiles					534:541	glycan profiles	527:541	glycan profiles of HA	527:547	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	2	6	theme	mass	290:293	arg1	spectrometry					295:306	mass spectrometry	290:306	mass spectrometry	290:306	However, the formerly established methods using mass spectrometry present disadvantages such as procedure complexity, sensitivity, and throughput.					
30503907	8	7	gly	glycoproteins	1056:1068	arg1	glycoproteins					1056:1068	the viral glycoproteins	1046:1068	the viral glycoproteins	1046:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	4	8	theme	simple	602:607	arg1	enrichment					617:626	simple antigen enrichment	602:626	simple antigen enrichment using optimized immunoprecipitation	602:662	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	0	9	theme	influenza	94:102	arg1	viruses					106:112	influenza A viruses	94:112	influenza A viruses	94:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	8	10	theme	glycan	1026:1031	arg1	profiling					1033:1041	glycan profiling	1026:1041	glycan profiling of the viral glycoproteins	1026:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	6	11	theme	glycan	888:893	arg1	profiles					895:902	the host cell-specific HA glycan profiles	862:902	the host cell-specific HA glycan profiles	862:902	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	1	12	theme	influenza	158:166	arg1	viruses					170:176	influenza A viruses	158:176	influenza A viruses	158:176	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	0	13	theme	viruses	106:112	arg1	hemagglutinins					76:89	the hemagglutinins	72:89	the hemagglutinins of influenza A viruses	72:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	6	14	theme	HA	885:886	arg1	profiles					895:902	the host cell-specific HA glycan profiles	862:902	the host cell-specific HA glycan profiles	862:902	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	0	15	theme	cell-specific	39:51	arg1	profiles					60:67	host cell-specific glycan profiles	34:67	host cell-specific glycan profiles of the hemagglutinins of influenza A viruses	34:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	7	16	theme	MDCK	926:929	arg1	cells					931:935	MDCK cells	926:935	MDCK cells	926:935	Notably, the HA from MDCK cells was α1-3 galactosylated.					
30503907	2	17	theme	established	264:274	arg1	methods					276:282	the formerly established methods	251:282	the formerly established methods using mass spectrometry	251:306	However, the formerly established methods using mass spectrometry present disadvantages such as procedure complexity, sensitivity, and throughput.					
30503907	1	18	theme	A	168:168	arg1	viruses					170:176	influenza A viruses	158:176	influenza A viruses	158:176	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	0	19	theme	microarray	7:16	arg1	analyses					18:25	Lectin microarray analyses	0:25	Lectin microarray analyses	0:25	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	6	20	theme	cell-specific	871:883	arg1	profiles					895:902	the host cell-specific HA glycan profiles	862:902	the host cell-specific HA glycan profiles	862:902	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	8	21	theme	glycoproteins	1056:1068	arg1	profiling					1033:1041	glycan profiling	1026:1041	glycan profiling of the viral glycoproteins	1026:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	1	22	theme	viruses	170:176	arg1	HA					151:152	HA	151:152	HA	151:152	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	1	22	theme	viruses	170:176	arg1	hemagglutinin					136:148	hemagglutinin	136:148	hemagglutinin (HA) of influenza A viruses	136:176	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	0	23	theme	Lectin	0:5	arg1	analyses					18:25	Lectin microarray analyses	0:25	Lectin microarray analyses	0:25	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	8	24	theme	viral	1050:1054	arg1	glycoproteins					1056:1068	the viral glycoproteins	1046:1068	the viral glycoproteins	1046:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	6	25	theme	chicken	800:806	arg1	embryos					808:814	chicken embryos	800:814	chicken embryos	800:814	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	6	25	theme	chicken	800:806	arg1	cells					838:842	A549 cells	833:842	A549 cells	833:842	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	6	25	theme	chicken	800:806	arg1	Vero					823:826	Vero	823:826	Vero	823:826	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	6	25	theme	chicken	800:806	arg1	MDCK					817:820	MDCK	817:820	MDCK	817:820	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	4	26	theme	antigen	609:615	arg1	enrichment					617:626	simple antigen enrichment	602:626	simple antigen enrichment using optimized immunoprecipitation	602:662	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	8	27	theme	simple	1005:1010	arg1	procedure					1012:1020	a highly sensitive and simple procedure	982:1020	a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins	982:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	5	28	theme	HA	737:738	arg1	structures					723:732	known glycan structures	710:732	known glycan structures of HA	710:738	The profiles were reasonably consistent with known glycan structures of HA.					
30503907	1	29	from	structures	122:131	arg1	HA					151:152	HA	151:152	HA	151:152	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	1	29	from	structures	122:131	arg1	hemagglutinin					136:148	hemagglutinin	136:148	hemagglutinin (HA) of influenza A viruses	136:176	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	6	30	theme	host	866:869	arg1	profiles					895:902	the host cell-specific HA glycan profiles	862:902	the host cell-specific HA glycan profiles	862:902	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	3	31	theme	lectin	472:477	arg1	techniques					490:499	lectin microarray techniques	472:499	lectin microarray techniques	472:499	Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques.					
30503907	3	32	theme	novel	417:421	arg1	method					423:428	a novel method	415:428	a novel method for analyzing glycan profiles of HA	415:464	Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques.					
30503907	0	33	theme	host	34:37	arg1	profiles					60:67	host cell-specific glycan profiles	34:67	host cell-specific glycan profiles of the hemagglutinins of influenza A viruses	34:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	4	34	theme	HA	546:547	arg1	profiles					534:541	glycan profiles	527:541	glycan profiles of HA	527:547	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	8	35	link	animal-derived	1131:1144	arg1	virions					1146:1152	human- and animal-derived virions	1120:1152	human- and animal-derived virions	1120:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	3	36	theme	HA	463:464	arg1	profiles					451:458	glycan profiles	444:458	glycan profiles of HA	444:464	Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques.					
30503907	5	37	theme	known	710:714	arg1	structures					723:732	known glycan structures	710:732	known glycan structures of HA	710:738	The profiles were reasonably consistent with known glycan structures of HA.					
30503907	3	38	theme	glycan	444:449	arg1	profiles					451:458	glycan profiles	444:458	glycan profiles of HA	444:464	Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques.					
30503907	1	39	theme	Glycan	115:120	arg1	structures					122:131	Glycan structures	115:131	Glycan structures on hemagglutinin (HA) of influenza A viruses	115:176	Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance.					
30503907	0	40	theme	glycan	53:58	arg1	profiles					60:67	host cell-specific glycan profiles	34:67	host cell-specific glycan profiles of the hemagglutinins of influenza A viruses	34:112	Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.					
30503907	5	41	theme	glycan	716:721	arg1	structures					723:732	known glycan structures	710:732	known glycan structures of HA	710:738	The profiles were reasonably consistent with known glycan structures of HA.					
30503907	6	42	theme	the	778:780	arg1	profiles					766:773	glycan profiles	759:773	glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells	759:842	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	8	43	theme	sensitive	991:999	arg1	procedure					1012:1020	a highly sensitive and simple procedure	982:1020	a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins	982:1068	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	2	44	theme	procedure	338:346	arg1	complexity					348:357	procedure complexity	338:357	procedure complexity	338:357	However, the formerly established methods using mass spectrometry present disadvantages such as procedure complexity, sensitivity, and throughput.					
30503907	8	45	theme	glycan	1101:1106	arg1	analyses					1108:1115	direct glycan analyses	1094:1115	direct glycan analyses of human- and animal-derived virions	1094:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	6	46	theme	A549	833:836	arg1	cells					838:842	A549 cells	833:842	A549 cells	833:842	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	6	46	theme	A549	833:836	arg1	embryos					808:814	chicken embryos	800:814	chicken embryos	800:814	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	7	47	from	cells	931:935	arg1	HA					918:919	the HA	914:919	the HA from MDCK cells	914:935	Notably, the HA from MDCK cells was α1-3 galactosylated.					
30503907	7	47	from	cells	931:935	arg1	galactosylated					946:959	galactosylated	946:959	galactosylated	946:959	Notably, the HA from MDCK cells was α1-3 galactosylated.					
30503907	3	48	theme	microarray	479:488	arg1	techniques					490:499	lectin microarray techniques	472:499	lectin microarray techniques	472:499	Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques.					
30503907	8	49	theme	human-	1120:1125	arg1	virions					1146:1152	human- and animal-derived virions	1120:1152	human- and animal-derived virions	1120:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
30503907	6	50	theme	glycan	759:764	arg1	profiles					766:773	glycan profiles	759:773	glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells	759:842	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	5	51	with	consistent	694:703	arg1	structures					723:732	known glycan structures	710:732	known glycan structures of HA	710:738	The profiles were reasonably consistent with known glycan structures of HA.					
30503907	4	52	theme	optimized	634:642	arg1	immunoprecipitation					644:662	optimized immunoprecipitation	634:662	optimized immunoprecipitation	634:662	We successfully obtained glycan profiles of HA starting from 1 ml of the 106 TCID50 samples through simple antigen enrichment using optimized immunoprecipitation.					
30503907	6	53	dep	the	778:780	arg1	HAs					782:784	HAs	782:784	HAs	782:784	Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles.					
30503907	8	54	theme	virions	1146:1152	arg1	analyses					1108:1115	direct glycan analyses	1094:1115	direct glycan analyses of human- and animal-derived virions	1094:1152	Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.					
31479666	0	0	theme	defined	77:83	arg1	oligosaccharides					93:108	structurally defined heparin oligosaccharides	64:108	structurally defined heparin oligosaccharides	64:108	Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.					
31479666	3	1	theme	sulfation	742:750	arg1	degrees					731:737	higher degrees	724:737	higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage	724:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	6	2	theme	heparin	1289:1295	arg1	depolymerization					1297:1312	heparin depolymerization	1289:1312	heparin depolymerization	1289:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	3	3	theme	higher	724:729	arg1	degrees					731:737	higher degrees	724:737	higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage	724:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	4	4	theme	glucuronic	950:959	arg1	acid					961:964	glucuronic acid	950:964	glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides	950:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	2	5	theme	glycosidic	367:376	arg1	linkages					378:385	five glycosidic linkages	362:385	five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites	362:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	0	6	theme	heparin	85:91	arg1	oligosaccharides					93:108	structurally defined heparin oligosaccharides	64:108	structurally defined heparin oligosaccharides	64:108	Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.					
31479666	6	7	from	information	1213:1223	arg1	promiscuity					1241:1251	the cleavage promiscuity	1228:1251	the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization	1228:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	3	8	theme	indispensable	776:788	arg1	glucosamine					804:814	indispensable N-substituted glucosamine	776:814	indispensable N-substituted glucosamine adjacent to the cleavable linkage	776:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	4	9	theme	glycosidic	919:928	arg1	linkages					930:937	glycosidic linkages	919:937	glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides	919:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	1	10	theme	I	192:192	arg1	specificity					156:166	the specificity	152:166	the specificity of bacterial heparinase I	152:192	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	1	11	theme	bacterial	171:179	arg1	I					192:192	bacterial heparinase I	171:192	bacterial heparinase I	171:192	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	2	12	theme	heparinase	510:519	arg1	concentration					493:505	a high concentration	486:505	a high concentration of heparinase I	486:521	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	5	13	theme	Biolayer	1039:1046	arg1	interferometry					1048:1061	Biolayer interferometry	1039:1061	Biolayer interferometry	1039:1061	Biolayer interferometry revealed differences in reactivity that are not completely consistent with different affinities of substrates to enzyme.					
31479666	4	14	contain	containing	977:986	arg2	acid					997:1000	iduronic acid	988:1000	iduronic acid of the counterpart oligosaccharides	988:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	4	14	contain	containing	977:986	arg1	those					971:975	those	971:975	those	971:975	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	1	15	theme	heparinase	181:190	arg1	I					192:192	bacterial heparinase I	171:192	bacterial heparinase I	171:192	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	3	16	theme	oligosaccharides	755:770	arg1	sulfation					742:750	sulfation	742:750	sulfation of oligosaccharides	742:770	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	3	16	theme	oligosaccharides	755:770	arg1	glucosamine					804:814	indispensable N-substituted glucosamine	776:814	indispensable N-substituted glucosamine adjacent to the cleavable linkage	776:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	3	17	theme	adjacent	816:823	arg1	glucosamine					804:814	indispensable N-substituted glucosamine	776:814	indispensable N-substituted glucosamine adjacent to the cleavable linkage	776:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	2	18	theme	primary	339:345	arg1	site					356:359	the primary cleavage site	335:359	the primary cleavage site	335:359	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	2	19	theme	varying	412:418	arg1	modifications					420:432	varying modifications	412:432	varying modifications to obtain secondary cleavage sites	412:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	1	20	theme	efficient	289:297	arg1	strategy					314:321	a highly efficient chemoenzymatic strategy	280:321	a highly efficient chemoenzymatic strategy	280:321	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	3	21	theme	2-O-sulfated	605:616	arg1	acid					627:630	the 2-O-sulfated iduronic acid	601:630	the 2-O-sulfated iduronic acid being cleaved	601:644	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	1	22	theme	chemoenzymatic	299:312	arg1	strategy					314:321	a highly efficient chemoenzymatic strategy	280:321	a highly efficient chemoenzymatic strategy	280:321	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	4	23	theme	counterpart	1009:1019	arg1	oligosaccharides					1021:1036	the counterpart oligosaccharides	1005:1036	the counterpart oligosaccharides	1005:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	5	24	theme	substrates	1162:1171	arg1	affinities					1148:1157	different affinities	1138:1157	different affinities of substrates to enzyme	1138:1181	Biolayer interferometry revealed differences in reactivity that are not completely consistent with different affinities of substrates to enzyme.					
31479666	6	25	theme	accurate	1204:1211	arg1	information					1213:1223	accurate information	1204:1223	accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization	1204:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	0	26	theme	detailed	15:22	arg1	mode					24:27	the detailed mode	11:27	the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides	11:108	Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.					
31479666	3	27	theme	N-substituted	790:802	arg1	glucosamine					804:814	indispensable N-substituted glucosamine	776:814	indispensable N-substituted glucosamine adjacent to the cleavable linkage	776:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	3	28	theme	linkages	542:549	arg1	reactivity					528:537	The reactivity	524:537	The reactivity of linkages toward heparinase I	524:569	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	3	28	theme	linkages	542:549	arg1	dependent					588:596	dependent	588:596	dependent	588:596	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	2	29	theme	cleavage	454:461	arg1	sites					463:467	secondary cleavage sites	444:467	secondary cleavage sites	444:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	0	30	theme	cleavage	32:39	arg1	mode					24:27	the detailed mode	11:27	the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides	11:108	Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.					
31479666	3	31	theme	glucosamine	804:814	arg1	degrees					731:737	higher degrees	724:737	higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage	724:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	5	32	theme	different	1138:1146	arg1	affinities					1148:1157	different affinities	1138:1157	different affinities of substrates to enzyme	1138:1181	Biolayer interferometry revealed differences in reactivity that are not completely consistent with different affinities of substrates to enzyme.					
31479666	2	33	theme	secondary	444:452	arg1	sites					463:467	secondary cleavage sites	444:467	secondary cleavage sites	444:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	4	34	theme	oligosaccharides	1021:1036	arg1	acid					997:1000	iduronic acid	988:1000	iduronic acid of the counterpart oligosaccharides	988:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	3	35	theme	glucosamine	678:688	arg1	residues					690:697	the neighboring 6-O-sulfated glucosamine residues	649:697	the neighboring 6-O-sulfated glucosamine residues	649:697	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	0	36	theme	heparinase	44:53	arg1	cleavage					32:39	cleavage	32:39	cleavage of heparinase I	32:55	Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.					
31479666	4	37	theme	iduronic	988:995	arg1	acid					997:1000	iduronic acid	988:1000	iduronic acid of the counterpart oligosaccharides	988:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	3	38	theme	iduronic	618:625	arg1	acid					627:630	the 2-O-sulfated iduronic acid	601:630	the 2-O-sulfated iduronic acid being cleaved	601:644	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	6	39	theme	heparinase	1256:1265	arg1	I					1267:1267	heparinase I	1256:1267	heparinase I that is crucial for heparin depolymerization	1256:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	2	40	theme	high	488:491	arg1	concentration					493:505	a high concentration	486:505	a high concentration of heparinase I	486:521	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	4	41	theme	preferential	890:901	arg1	cleavage					903:910	less preferential cleavage	885:910	less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides	885:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	6	42	theme	I	1267:1267	arg1	promiscuity					1241:1251	the cleavage promiscuity	1228:1251	the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization	1228:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	3	43	theme	cleavable	832:840	arg1	linkage					842:848	the cleavable linkage	828:848	the cleavable linkage	828:848	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	3	44	theme	neighboring	653:663	arg1	residues					690:697	the neighboring 6-O-sulfated glucosamine residues	649:697	the neighboring 6-O-sulfated glucosamine residues	649:697	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	1	45	theme	insightful	122:131	arg1	investigation					133:145	a more insightful investigation	115:145	a more insightful investigation into the specificity of bacterial heparinase I	115:192	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	4	46	contain	containing	939:948	arg1	linkages					930:937	glycosidic linkages	919:937	glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides	919:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	4	46	contain	containing	939:948	arg2	acid					961:964	glucuronic acid	950:964	glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides	950:1036	Moreover, the enzyme demonstrated less preferential cleavage toward glycosidic linkages containing glucuronic acid than those containing iduronic acid of the counterpart oligosaccharides.					
31479666	1	47	theme	well-defined	220:231	arg1	oligosaccharides					241:256	structurally well-defined heparin oligosaccharides	207:256	structurally well-defined heparin oligosaccharides	207:256	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	2	48	with	oligosaccharides	390:405	arg1	modifications					420:432	varying modifications	412:432	varying modifications to obtain secondary cleavage sites	412:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	3	49	theme	6-O-sulfated	665:676	arg1	residues					690:697	the neighboring 6-O-sulfated glucosamine residues	649:697	the neighboring 6-O-sulfated glucosamine residues	649:697	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31479666	2	50	theme	cleavage	347:354	arg1	site					356:359	the primary cleavage site	335:359	the primary cleavage site	335:359	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	1	51	theme	heparin	233:239	arg1	oligosaccharides					241:256	structurally well-defined heparin oligosaccharides	207:256	structurally well-defined heparin oligosaccharides	207:256	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	5	52	with	consistent	1122:1131	arg1	affinities					1148:1157	different affinities	1138:1157	different affinities of substrates to enzyme	1138:1181	Biolayer interferometry revealed differences in reactivity that are not completely consistent with different affinities of substrates to enzyme.					
31479666	6	53	theme	cleavage	1232:1239	arg1	promiscuity					1241:1251	the cleavage promiscuity	1228:1251	the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization	1228:1312	Our study presented accurate information on the cleavage promiscuity of heparinase I that is crucial for heparin depolymerization.					
31479666	2	54	theme	oligosaccharides	390:405	arg1	linkages					378:385	five glycosidic linkages	362:385	five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites	362:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	5	55	from	differences	1072:1082	arg1	reactivity					1087:1096	reactivity	1087:1096	reactivity	1087:1096	Biolayer interferometry revealed differences in reactivity that are not completely consistent with different affinities of substrates to enzyme.					
31479666	1	56	theme	oligosaccharides	241:256	arg1	series					197:202	a series	195:202	a series of structurally well-defined heparin oligosaccharides	195:256	For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy.					
31479666	2	57	dep	modifications	420:432	arg1	obtain					437:442	obtain	437:442	to obtain secondary cleavage sites	434:467	Apart from the primary cleavage site, five glycosidic linkages of oligosaccharides with varying modifications to obtain secondary cleavage sites were degraded by a high concentration of heparinase I.					
31479666	3	58	theme	heparinase	558:567	arg1	I					569:569	heparinase I	558:569	heparinase I	558:569	The reactivity of linkages toward heparinase I was not entirely dependent on the 2-O-sulfated iduronic acid being cleaved or the neighboring 6-O-sulfated glucosamine residues, but it was dependent on higher degrees of sulfation of oligosaccharides and indispensable N-substituted glucosamine adjacent to the cleavable linkage.					
31437391	0	0	theme	Liquid	81:86	arg1	Chromatography					88:101	Liquid Chromatography	81:101	Liquid Chromatography	81:101	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	3	1	theme	proteins	664:671	arg1	concentration					647:659	the low concentration	639:659	the low concentration of proteins in CSF	639:678	However, due to the low concentration of proteins in CSF, a large volume of the biofluid is often required to comprehensively characterize the glycome in CSF.					
31437391	2	2	theme	due	590:592	arg1	changes					582:588	significant biochemical and neurochemical changes	540:588	significant biochemical and neurochemical changes due to the disease	540:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	5	3	theme	liquid	1027:1032	arg1	chromatography					1034:1047	liquid chromatography	1027:1047	liquid chromatography	1027:1047	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	1	4	theme	protein	174:180	arg1	Glycosylation					127:139	Glycosylation	127:139	Glycosylation	127:139	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	4	theme	protein	174:180	arg1	modification					182:193	an essential post-translational protein modification	142:193	an essential post-translational protein modification	142:193	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	2	5	theme	condition	467:475	arg1	progression					445:455	the progression	441:455	the progression of such a condition	441:475	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	0	6	from	Profile	9:15	arg1	Patients					66:73	Alzheimer's Disease Patients	46:73	Alzheimer's Disease Patients	46:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	5	7	theme	purification	995:1006	arg1	system					1008:1013	an online purification system	985:1013	an online purification system attached to liquid chromatography and a high-resolution mass spectrometer	985:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	1	8	theme	neurodegenerative	255:271	arg1	disease					302:308	Alzheimer's disease	290:308	Alzheimer's disease (AD)	290:313	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	8	theme	neurodegenerative	255:271	arg1	diseases					273:280	neurodegenerative diseases	255:280	neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments	255:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	5	9	theme	high-resolution	1055:1069	arg1	spectrometer					1076:1087	a high-resolution mass spectrometer	1053:1087	a high-resolution mass spectrometer	1053:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	2	10	theme	neurochemical	568:580	arg1	changes					582:588	significant biochemical and neurochemical changes	540:588	significant biochemical and neurochemical changes due to the disease	540:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	7	11	theme	lower	1302:1306	arg1	abundances					1308:1317	lower abundances	1302:1317	lower abundances of high-mannose structures	1302:1344	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	7	12	theme	Preliminary	1143:1153	arg1	data					1155:1158	Preliminary data	1143:1158	Preliminary data	1143:1158	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	3	13	theme	low	643:645	arg1	concentration					647:659	the low concentration	639:659	the low concentration of proteins in CSF	639:678	However, due to the low concentration of proteins in CSF, a large volume of the biofluid is often required to comprehensively characterize the glycome in CSF.					
31437391	5	14	with	permethylation	899:912	arg1	cleanup					956:962	minimal sample cleanup	941:962	minimal sample cleanup	941:962	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	7	15	theme	fucosylated	1173:1183	arg1	structures					1206:1215	fucosylated and bisecting GlcNAc structures	1173:1215	structures	1206:1215	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	8	16	theme	disease	1420:1426	arg1	state					1428:1432	disease state	1420:1432	disease state	1420:1432	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	5	17	with	conjunction	968:978	arg1	system					1008:1013	an online purification system	985:1013	an online purification system attached to liquid chromatography and a high-resolution mass spectrometer	985:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	4	18	theme	glycomic	798:805	arg1	study					807:811	a glycomic study	796:811	a glycomic study of CSF	796:818	In this work, a glycomic study of CSF was performed using as little as 10 μL of CSF.					
31437391	0	19	theme	Mass	108:111	arg1	Spectrometry					113:124	Mass Spectrometry	108:124	Mass Spectrometry	108:124	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	1	20	theme	common	341:346	arg1	disorders					366:374	the most common neurodegenerative disorders	332:374	the most common neurodegenerative disorders	332:374	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	21	theme	neurodegenerative	348:364	arg1	disorders					366:374	the most common neurodegenerative disorders	332:374	the most common neurodegenerative disorders	332:374	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	4	22	theme	CSF	816:818	arg1	study					807:811	a glycomic study	796:811	a glycomic study of CSF	796:818	In this work, a glycomic study of CSF was performed using as little as 10 μL of CSF.					
31437391	0	23	theme	N-Glycan	0:7	arg1	Profile					9:15	N-Glycan Profile	0:15	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients	0:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	8	24	theme	significant	1388:1398	arg1	differences					1400:1410	statistically significant differences	1374:1410	statistically significant differences between disease state and disease-free CSF	1374:1453	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	0	25	theme	Cerebrospinal	20:32	arg1	Fluids					34:39	Cerebrospinal Fluids	20:39	Cerebrospinal Fluids from Alzheimer's Disease Patients	20:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	8	26	theme	samples	1485:1491	arg1	number					1475:1480	number	1475:1480	number of samples	1475:1491	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	1	27	gly	Glycosylation	127:139	arg1	diseases					235:242	diseases	235:242	diseases	235:242	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	27	gly	Glycosylation	127:139	arg1	variety					224:230	a variety	222:230	a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments	222:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	27	gly	Glycosylation	127:139	arg1	diseases					273:280	neurodegenerative diseases	255:280	neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments	255:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	7	28	theme	structures	1335:1344	arg1	abundances					1308:1317	lower abundances	1302:1317	lower abundances of high-mannose structures	1302:1344	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	2	29	contain	possess	532:538	arg1	biofluid					514:521	a unique biofluid	505:521	a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease	505:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	29	contain	possess	532:538	arg1	fluid					492:496	cerebrospinal fluid	478:496	cerebrospinal fluid (CSF)	478:502	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	29	contain	possess	532:538	arg2	changes					582:588	significant biochemical and neurochemical changes	540:588	significant biochemical and neurochemical changes due to the disease	540:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	3	30	theme	large	683:687	arg1	volume					689:694	a large volume	681:694	a large volume of the biofluid	681:710	However, due to the low concentration of proteins in CSF, a large volume of the biofluid is often required to comprehensively characterize the glycome in CSF.					
31437391	1	31	theme	disorders	366:374	arg1	one					325:327	one	325:327	one	325:327	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	31	theme	disorders	366:374	arg1	disorders					366:374	the most common neurodegenerative disorders	332:374	the most common neurodegenerative disorders	332:374	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	8	32	theme	validation	1502:1511	arg1	study					1513:1517	further validation study	1494:1517	further validation study	1494:1517	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	5	33	attach	attached	1015:1022	arg2	system					1008:1013	an online purification system	985:1013	an online purification system attached to liquid chromatography and a high-resolution mass spectrometer	985:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	5	33	attach	attached	1015:1022	arg1	spectrometer					1076:1087	a high-resolution mass spectrometer	1053:1087	a high-resolution mass spectrometer	1053:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	5	33	attach	attached	1015:1022	arg1	chromatography					1034:1047	liquid chromatography	1027:1047	liquid chromatography	1027:1047	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	8	34	theme	further	1494:1500	arg1	study					1513:1517	further validation study	1494:1517	further validation study	1494:1517	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	2	35	theme	unique	507:512	arg1	biofluid					514:521	a unique biofluid	505:521	a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease	505:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	35	theme	unique	507:512	arg1	fluid					492:496	cerebrospinal fluid	478:496	cerebrospinal fluid (CSF)	478:502	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	7	36	theme	bisecting	1189:1197	arg1	structures					1206:1215	fucosylated and bisecting GlcNAc structures	1173:1215	structures	1206:1215	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	5	37	theme	N-glycans	926:934	arg1	permethylation					899:912	permethylation	899:912	permethylation of released N-glycans with minimal sample cleanup	899:962	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	7	38	with	females	1246:1252	arg1	AD					1259:1260	AD	1259:1260	AD	1259:1260	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	0	39	theme	Fluids	34:39	arg1	Profile					9:15	N-Glycan Profile	0:15	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients	0:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	5	40	theme	mass	1071:1074	arg1	spectrometer					1076:1087	a high-resolution mass spectrometer	1053:1087	a high-resolution mass spectrometer	1053:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	3	41	from	concentration	647:659	arg1	CSF					676:678	CSF	676:678	CSF	676:678	However, due to the low concentration of proteins in CSF, a large volume of the biofluid is often required to comprehensively characterize the glycome in CSF.					
31437391	0	42	from	Patients	66:73	arg1	Profile					9:15	N-Glycan Profile	0:15	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients	0:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	0	42	from	Patients	66:73	arg1	Fluids					34:39	Cerebrospinal Fluids	20:39	Cerebrospinal Fluids from Alzheimer's Disease Patients	20:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	7	43	theme	high-mannose	1322:1333	arg1	structures					1335:1344	high-mannose structures	1322:1344	high-mannose structures	1322:1344	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	7	44	from	abundances	1232:1241	arg1	females					1246:1252	females	1246:1252	females with AD	1246:1260	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	4	45	theme	CSF	862:864	arg1	μL					856:857	as little as 10 μL	840:857	as little as 10 μL of CSF	840:864	In this work, a glycomic study of CSF was performed using as little as 10 μL of CSF.					
31437391	2	46	theme	biochemical	552:562	arg1	changes					582:588	significant biochemical and neurochemical changes	540:588	significant biochemical and neurochemical changes due to the disease	540:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	47	theme	cerebrospinal	478:490	arg1	biofluid					514:521	a unique biofluid	505:521	a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease	505:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	47	theme	cerebrospinal	478:490	arg1	CSF					499:501	CSF	499:501	CSF	499:501	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	47	theme	cerebrospinal	478:490	arg1	fluid					492:496	cerebrospinal fluid	478:496	cerebrospinal fluid (CSF)	478:502	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	5	48	theme	minimal	941:947	arg1	cleanup					956:962	minimal sample cleanup	941:962	minimal sample cleanup	941:962	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	7	49	gly	fucosylated	1173:1183	arg1	structures					1206:1215	fucosylated and bisecting GlcNAc structures	1173:1215	structures	1206:1215	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	3	50	theme	biofluid	703:710	arg1	volume					689:694	a large volume	681:694	a large volume of the biofluid	681:710	However, due to the low concentration of proteins in CSF, a large volume of the biofluid is often required to comprehensively characterize the glycome in CSF.					
31437391	2	51	theme	significant	540:550	arg1	changes					582:588	significant biochemical and neurochemical changes	540:588	significant biochemical and neurochemical changes due to the disease	540:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	1	52	theme	cognitive	392:400	arg1	impairments					413:423	cognitive and memory impairments	392:423	impairments	413:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	5	53	theme	sample	949:954	arg1	cleanup					956:962	minimal sample cleanup	941:962	minimal sample cleanup	941:962	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	5	54	theme	online	988:993	arg1	system					1008:1013	an online purification system	985:1013	an online purification system attached to liquid chromatography and a high-resolution mass spectrometer	985:1087	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	7	55	theme	GlcNAc	1199:1204	arg1	structures					1206:1215	fucosylated and bisecting GlcNAc structures	1173:1215	structures	1206:1215	Preliminary data suggest that fucosylated and bisecting GlcNAc structures were higher in abundances in females with AD, while both females and males exhibited lower abundances of high-mannose structures.					
31437391	1	56	theme	memory	406:411	arg1	impairments					413:423	cognitive and memory impairments	392:423	impairments	413:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	57	theme	post-translational	155:172	arg1	Glycosylation					127:139	Glycosylation	127:139	Glycosylation	127:139	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	57	theme	post-translational	155:172	arg1	modification					182:193	an essential post-translational protein modification	142:193	an essential post-translational protein modification	142:193	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	8	58	theme	disease-free	1438:1449	arg1	CSF					1451:1453	disease-free CSF	1438:1453	disease-free CSF	1438:1453	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	6	59	theme	clinical	1125:1132	arg1	samples					1134:1140	clinical samples	1125:1140	clinical samples	1125:1140	This technique was then applied to clinical samples.					
31437391	8	60	theme	number	1475:1480	arg1	lack					1467:1470	the lack	1463:1470	the lack of number of samples	1463:1491	Although there seems to be statistically significant differences between disease state and disease-free CSF, due to the lack of number of samples, further validation study should be conducted.					
31437391	1	61	theme	essential	145:153	arg1	Glycosylation					127:139	Glycosylation	127:139	Glycosylation	127:139	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	61	theme	essential	145:153	arg1	modification					182:193	an essential post-translational protein modification	142:193	an essential post-translational protein modification	142:193	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	62	theme	diseases	235:242	arg1	diseases					235:242	diseases	235:242	diseases	235:242	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	62	theme	diseases	235:242	arg1	variety					224:230	a variety	222:230	a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments	222:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	1	62	theme	diseases	235:242	arg1	diseases					273:280	neurodegenerative diseases	255:280	neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments	255:423	Glycosylation, an essential post-translational protein modification, is known to be altered in a variety of diseases, including neurodegenerative diseases such as Alzheimer's disease (AD), which is one of the most common neurodegenerative disorders that results in cognitive and memory impairments.					
31437391	0	63	theme	Disease	58:64	arg1	Patients					66:73	Alzheimer's Disease Patients	46:73	Alzheimer's Disease Patients	46:73	N-Glycan Profile of Cerebrospinal Fluids from Alzheimer's Disease Patients Using Liquid Chromatography with Mass Spectrometry.					
31437391	5	64	theme	released	917:924	arg1	N-glycans					926:934	released N-glycans	917:934	released N-glycans	917:934	This approach was executed with permethylation of released N-glycans with minimal sample cleanup, in conjunction with an online purification system attached to liquid chromatography and a high-resolution mass spectrometer.					
31437391	2	65	used	utilized	613:620	arg2	CSF					499:501	CSF	499:501	CSF	499:501	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	65	used	utilized	613:620	arg2	fluid					492:496	cerebrospinal fluid	478:496	cerebrospinal fluid (CSF)	478:502	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31437391	2	65	used	utilized	613:620	arg2	biofluid					514:521	a unique biofluid	505:521	a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease	505:607	To investigate the progression of such a condition, cerebrospinal fluid (CSF), a unique biofluid that may possess significant biochemical and neurochemical changes due to the disease, is utilized.					
31306700	5	0	theme	human	740:744	arg1	tissues					753:759	human breast tissues	740:759	human breast tissues	740:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	0	1	from	glycophenotype	15:28	arg1	tissues					47:53	breast cancer tissues	33:53	breast cancer tissues with quantum dots-Cramoll lectin conjugates	33:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	10	2	theme	glucose/mannose	1316:1330	arg1	residues					1332:1339	exposed glucose/mannose residues	1308:1339	exposed glucose/mannose residues	1308:1339	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	1	3	theme	many	165:168	arg1	processes					181:189	many biological processes	165:189	many biological processes	165:189	During carcinogenesis, changes in the glycosylation can modulate many biological processes.					
31306700	11	4	theme	glycan	1506:1511	arg1	profiles					1513:1520	glycan profiles	1506:1520	glycan profiles in normal and transformed tissues	1506:1554	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	0	5	theme	lectin	81:86	arg1	conjugates					88:97	quantum dots-Cramoll lectin conjugates	60:97	quantum dots-Cramoll lectin conjugates	60:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	10	6	theme	malignancy	1377:1386	arg1	degree					1388:1393	the sample malignancy degree	1366:1393	the sample malignancy degree	1366:1393	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	1	7	theme	biological	170:179	arg1	processes					181:189	many biological processes	165:189	many biological processes	165:189	During carcinogenesis, changes in the glycosylation can modulate many biological processes.					
31306700	4	8	theme	glucose/mannose	567:581	arg1	residues					583:590	glucose/mannose residues	567:590	glucose/mannose residues	567:590	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	5	9	theme	invasive	708:715	arg1	carcinoma					724:732	invasive ductal carcinoma	708:732	invasive ductal carcinoma (IDC)	708:738	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	5	9	theme	invasive	708:715	arg1	IDC					735:737	IDC	735:737	IDC	735:737	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	8	10	theme	lectin	956:961	arg1	activity					963:970	The lectin activity	952:970	The lectin activity	952:970	The lectin activity and secondary structure were also preserved after the conjugation with QDs.					
31306700	11	11	theme	FMA	1595:1597	arg1	quantification					1599:1612	FMA quantification	1595:1612	fluorescence microscopy as well as FMA quantification	1560:1612	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	5	12	theme	ductal	717:722	arg1	carcinoma					724:732	invasive ductal carcinoma	708:732	invasive ductal carcinoma (IDC)	708:738	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	5	12	theme	ductal	717:722	arg1	IDC					735:737	IDC	735:737	IDC	735:737	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	4	13	theme	dots	499:502	arg1	properties					477:486	the physicochemical properties	457:486	the physicochemical properties of quantum dots (QDs)	457:508	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	8	14	theme	secondary	976:984	arg1	structure					986:994	secondary structure	976:994	secondary structure	976:994	The lectin activity and secondary structure were also preserved after the conjugation with QDs.					
31306700	12	15	theme	fluorescent	1695:1705	arg1	conjugates					1707:1716	other fluorescent conjugates	1689:1716	other fluorescent conjugates	1689:1716	Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates.					
31306700	9	16	theme	fluorescence	1058:1069	arg1	images					1071:1076	fluorescence images	1058:1076	fluorescence images	1058:1076	Moreover, fluorescence images showed that ductal cells of normal and FB tissues were preferentially labeled by conjugates, whereas both cells and stroma were strongly labeled in IDC.					
31306700	10	17	theme	quantitative	1247:1258	arg1	way					1286:1288	a quantitative, practical, and sensitive way	1245:1288	a quantitative, practical, and sensitive way	1245:1288	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	5	18	theme	carcinoma	724:732	arg1	glycocode					664:672	the glycocode	660:672	the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues	660:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	4	19	theme	quantum	491:497	arg1	QDs					505:507	QDs	505:507	QDs	505:507	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	4	19	theme	quantum	491:497	arg1	dots					499:502	quantum dots	491:502	quantum dots (QDs)	491:508	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	8	20	with	conjugation	1026:1036	arg1	QDs					1043:1045	QDs	1043:1045	QDs	1043:1045	The lectin activity and secondary structure were also preserved after the conjugation with QDs.					
31306700	6	21	theme	fluorescence	859:870	arg1	FMA					890:892	FMA	890:892	FMA	890:892	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	6	21	theme	fluorescence	859:870	arg1	assay					883:887	a fluorescence microplate assay	857:887	a fluorescence microplate assay (FMA)	857:893	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	6	22	theme	tissue	828:833	arg1	intensity					844:852	the tissue labeling intensity	824:852	the tissue labeling intensity	824:852	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	12	23	theme	potential	1647:1655	arg1	FMA					1628:1630	FMA	1628:1630	FMA	1628:1630	Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates.					
31306700	12	23	theme	potential	1647:1655	arg1	method					1657:1662	a potential method	1645:1662	a potential method that can be applied with other fluorescent conjugates	1645:1716	Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates.					
31306700	5	24	dep	normal	677:682	arg1	tissues					753:759	human breast tissues	740:759	human breast tissues	740:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	5	25	theme	breast	746:751	arg1	tissues					753:759	human breast tissues	740:759	human breast tissues	740:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	0	26	theme	dots-Cramoll	68:79	arg1	conjugates					88:97	quantum dots-Cramoll lectin conjugates	60:97	quantum dots-Cramoll lectin conjugates	60:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	6	27	theme	microplate	872:881	arg1	FMA					890:892	FMA	890:892	FMA	890:892	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	6	27	theme	microplate	872:881	arg1	assay					883:887	a fluorescence microplate assay	857:887	a fluorescence microplate assay (FMA)	857:893	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	3	28	theme	fluorescent	402:412	arg1	reporters					414:422	fluorescent reporters	402:422	fluorescent reporters	402:422	Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent reporters.					
31306700	1	29	from	changes	123:129	arg1	glycosylation					138:150	the glycosylation	134:150	the glycosylation	134:150	During carcinogenesis, changes in the glycosylation can modulate many biological processes.					
31306700	9	30	theme	ductal	1090:1095	arg1	cells					1097:1101	ductal cells	1090:1101	ductal cells of normal and FB tissues	1090:1126	Moreover, fluorescence images showed that ductal cells of normal and FB tissues were preferentially labeled by conjugates, whereas both cells and stroma were strongly labeled in IDC.					
31306700	11	31	theme	transformed	1536:1546	arg1	tissues					1548:1554	normal and transformed tissues	1525:1554	normal and transformed tissues	1525:1554	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	11	32	theme	normal	1525:1530	arg1	tissues					1548:1554	normal and transformed tissues	1525:1554	normal and transformed tissues	1525:1554	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	11	33	theme	fluorescence	1560:1571	arg1	microscopy					1573:1582	fluorescence microscopy	1560:1582	fluorescence microscopy as well as FMA quantification	1560:1612	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	4	34	theme	physicochemical	461:475	arg1	properties					477:486	the physicochemical properties	457:486	the physicochemical properties of quantum dots (QDs)	457:508	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	10	35	theme	sensitive	1276:1284	arg1	way					1286:1288	a quantitative, practical, and sensitive way	1245:1288	a quantitative, practical, and sensitive way	1245:1288	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	11	36	theme	effective	1452:1460	arg1	probes					1487:1492	effective, specific, and versatile probes	1452:1492	effective, specific, and versatile probes	1452:1492	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	0	37	theme	cancer	40:45	arg1	tissues					47:53	breast cancer tissues	33:53	breast cancer tissues with quantum dots-Cramoll lectin conjugates	33:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	10	38	theme	practical	1261:1269	arg1	way					1286:1288	a quantitative, practical, and sensitive way	1245:1288	a quantitative, practical, and sensitive way	1245:1288	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	9	39	theme	normal	1106:1111	arg1	tissues					1120:1126	normal and FB tissues	1106:1126	normal and FB tissues	1106:1126	Moreover, fluorescence images showed that ductal cells of normal and FB tissues were preferentially labeled by conjugates, whereas both cells and stroma were strongly labeled in IDC.					
31306700	0	40	theme	breast	33:38	arg1	tissues					47:53	breast cancer tissues	33:53	breast cancer tissues with quantum dots-Cramoll lectin conjugates	33:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	6	41	theme	labeling	835:842	arg1	intensity					844:852	the tissue labeling intensity	824:852	the tissue labeling intensity	824:852	Additionally, we proposed a method to quantitatively evaluate the tissue labeling intensity by a fluorescence microplate assay (FMA).					
31306700	10	42	theme	exposed	1308:1314	arg1	residues					1332:1339	exposed glucose/mannose residues	1308:1339	exposed glucose/mannose residues	1308:1339	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	5	43	theme	normal	677:682	arg1	glycocode					664:672	the glycocode	660:672	the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues	660:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	4	44	theme	properties	477:486	arg1	advantage					444:452	advantage	444:452	advantage of the physicochemical properties of quantum dots (QDs)	444:508	Therefore, to take advantage of the physicochemical properties of quantum dots (QDs), herein, we conjugated Cramoll, a lectin that recognizes glucose/mannose residues, with those nanoparticles.					
31306700	9	45	theme	FB	1117:1118	arg1	tissues					1120:1126	normal and FB tissues	1106:1126	normal and FB tissues	1106:1126	Moreover, fluorescence images showed that ductal cells of normal and FB tissues were preferentially labeled by conjugates, whereas both cells and stroma were strongly labeled in IDC.					
31306700	11	46	theme	QDs-Cramoll	1411:1421	arg1	conjugates					1423:1432	QDs-Cramoll conjugates	1411:1432	QDs-Cramoll conjugates	1411:1432	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	9	47	theme	tissues	1120:1126	arg1	cells					1097:1101	ductal cells	1090:1101	ductal cells of normal and FB tissues	1090:1126	Moreover, fluorescence images showed that ductal cells of normal and FB tissues were preferentially labeled by conjugates, whereas both cells and stroma were strongly labeled in IDC.					
31306700	5	48	theme	fibroadenoma	685:696	arg1	glycocode					664:672	the glycocode	660:672	the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues	660:759	We applied the conjugates to investigate the glycocode of normal, fibroadenoma (FB), and invasive ductal carcinoma (IDC) human breast tissues.					
31306700	7	49	theme	intense	914:920	arg1	fluorescence					922:933	intense fluorescence	914:933	intense fluorescence	914:933	Conjugates showed intense fluorescence and specificity.					
31306700	2	50	theme	cancer	272:277	arg1	biomarkers					279:288	cancer biomarkers	272:288	cancer biomarkers	272:288	Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing.					
31306700	2	50	theme	cancer	272:277	arg1	roles					246:250	the roles	242:250	the roles of carbohydrates	242:267	Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing.					
31306700	10	51	theme	sample	1370:1375	arg1	degree					1388:1393	the sample malignancy degree	1366:1393	the sample malignancy degree	1366:1393	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
31306700	11	52	theme	versatile	1477:1485	arg1	probes					1487:1492	effective, specific, and versatile probes	1452:1492	effective, specific, and versatile probes	1452:1492	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	11	53	theme	specific	1463:1470	arg1	probes					1487:1492	effective, specific, and versatile probes	1452:1492	effective, specific, and versatile probes	1452:1492	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	3	54	theme	useful	340:345	arg1	Lectins					311:317	Lectins	311:317	Lectins	311:317	Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent reporters.					
31306700	3	54	theme	useful	340:345	arg1	tools					347:351	useful tools	340:351	useful tools in glycobiology	340:367	Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent reporters.					
31306700	3	55	from	tools	347:351	arg1	glycobiology					356:367	glycobiology	356:367	glycobiology	356:367	Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent reporters.					
31306700	2	56	theme	carbohydrates	255:267	arg1	biomarkers					279:288	cancer biomarkers	272:288	cancer biomarkers	272:288	Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing.					
31306700	2	56	theme	carbohydrates	255:267	arg1	roles					246:250	the roles	242:250	the roles of carbohydrates	242:267	Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing.					
31306700	0	57	with	tissues	47:53	arg1	conjugates					88:97	quantum dots-Cramoll lectin conjugates	60:97	quantum dots-Cramoll lectin conjugates	60:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	11	58	from	profiles	1513:1520	arg1	tissues					1548:1554	normal and transformed tissues	1525:1554	normal and transformed tissues	1525:1554	In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification.					
31306700	12	59	theme	other	1689:1693	arg1	conjugates					1707:1716	other fluorescent conjugates	1689:1716	other fluorescent conjugates	1689:1716	Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates.					
31306700	0	60	theme	quantum	60:66	arg1	conjugates					88:97	quantum dots-Cramoll lectin conjugates	60:97	quantum dots-Cramoll lectin conjugates	60:97	Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.					
31306700	10	61	theme	residues	1332:1339	arg1	level					1299:1303	the level	1295:1303	the level of exposed glucose/mannose residues	1295:1339	FMA showed in a quantitative, practical, and sensitive way that the level of exposed glucose/mannose residues increased accordingly to the sample malignancy degree.					
30848132	0	0	theme	Intestinal	27:36	arg1	Glycome					38:44	the Arctic Charr Intestinal Glycome	10:44	the Arctic Charr Intestinal Glycome	10:44	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	4	1	from	O-glycans	518:526	arg1	mucins					531:536	mucins	531:536	mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr	531:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	7	2	contain	had	1036:1038	arg2	number					1050:1055	lower (i) number	1040:1055	lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans	1040:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	7	2	contain	had	1036:1038	arg1	charr					1002:1006	Arctic charr	995:1006	Arctic charr fed with soy bean meal diet	995:1034	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	7	3	theme	interindividual	1086:1100	arg1	variation					1102:1110	(ii) interindividual variation	1081:1110	(ii) interindividual variation	1081:1110	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	6	4	theme	charr	957:961	arg1	glycosylation					980:992	Arctic charr intestinal mucin glycosylation	950:992	Arctic charr intestinal mucin glycosylation	950:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	8	5	theme	inflamed	1276:1283	arg1	charr					1292:1296	inflamed Arctic charr	1276:1296	inflamed Arctic charr	1276:1296	Furthermore, Aeromonas salmonicida grew less in response to mucins from inflamed Arctic charr than from the control group.					
30848132	5	6	theme	Arctic	720:725	arg1	charr					727:731	Arctic charr	720:731	Arctic charr intestinal mucins	720:749	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	2	7	theme	mucus	308:312	arg1	layer					314:318	a mucus layer	306:318	a mucus layer mainly comprised by highly glycosylated proteins called mucins	306:381	The intestinal tract is covered by a mucus layer mainly comprised by highly glycosylated proteins called mucins.					
30848132	6	8	theme	glycosylation	980:992	arg1	structures					849:858	Disialic-acid-epitope-containing structures	816:858	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc	816:927	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	8	theme	glycosylation	980:992	arg1	α2,8NeuGc					895:903	NeuAc(Gc)α2,8NeuGc	886:903	NeuAc(Gc)α2,8NeuGc(Ac)	886:907	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	8	theme	glycosylation	980:992	arg1	NeuGcα2,8NeuGc					914:927	NeuGcα2,8NeuGc	914:927	NeuGcα2,8NeuGc	914:927	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	8	theme	glycosylation	980:992	arg1	hallmark					938:945	the hallmark	934:945	the hallmark of Arctic charr intestinal mucin glycosylation	934:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	8	theme	glycosylation	980:992	arg1	NeuAcα2,8NeuAc					870:883	NeuAcα2,8NeuAc	870:883	NeuAcα2,8NeuAc	870:883	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	7	9	theme	structures	1060:1069	arg1	number					1050:1055	lower (i) number	1040:1055	lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans	1040:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	6	10	theme	intestinal	963:972	arg1	glycosylation					980:992	Arctic charr intestinal mucin glycosylation	950:992	Arctic charr intestinal mucin glycosylation	950:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	7	11	theme	meal	1026:1029	arg1	diet					1031:1034	soy bean meal diet	1017:1034	soy bean meal diet	1017:1034	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	6	12	theme	Arctic	950:955	arg1	charr					957:961	Arctic charr	950:961	Arctic charr intestinal mucin glycosylation	950:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	8	13	theme	control	1312:1318	arg1	group					1320:1324	the control group	1308:1324	the control group	1308:1324	Furthermore, Aeromonas salmonicida grew less in response to mucins from inflamed Arctic charr than from the control group.					
30848132	2	14	theme	intestinal	275:284	arg1	tract					286:290	The intestinal tract	271:290	The intestinal tract	271:290	The intestinal tract is covered by a mucus layer mainly comprised by highly glycosylated proteins called mucins.					
30848132	8	15	from	charr	1292:1296	arg1	mucins					1264:1269	mucins	1264:1269	mucins from inflamed Arctic charr	1264:1296	Furthermore, Aeromonas salmonicida grew less in response to mucins from inflamed Arctic charr than from the control group.					
30848132	0	16	from	Levels	95:100	arg1	Response					144:151	Response	144:151	Response to Inflammation	144:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	0	17	theme	Arctic	14:19	arg1	Charr					21:25	the Arctic Charr	10:25	the Arctic Charr Intestinal Glycome	10:44	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	4	18	theme	intestinal	501:510	arg1	O-glycans					518:526	intestinal mucin O-glycans	501:526	intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr	501:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	0	19	theme	N-Glycolylneuraminic	69:88	arg1	Acid					90:93	N-Glycolylneuraminic Acid	69:93	Increased N-Glycolylneuraminic Acid Levels	59:100	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	7	20	theme	Arctic	995:1000	arg1	charr					1002:1006	Arctic charr	995:1006	Arctic charr fed with soy bean meal diet	995:1034	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	10	21	theme	acid	1449:1452	arg1	biomarker					1463:1471	a biomarker	1461:1471	a biomarker for inflammation in Arctic char	1461:1503	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	10	21	theme	acid	1449:1452	arg1	loss					1420:1423	the loss	1416:1423	the loss of N-glycolylneuraminic acid	1416:1452	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	0	22	theme	Levels	95:100	arg1	Evidence					47:54	Evidence	47:54	Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation	47:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	0	23	theme	Changed	106:112	arg1	Interactions					128:139	Changed Host-Pathogen Interactions	106:139	Changed Host-Pathogen Interactions	106:139	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	0	24	theme	Increased	59:67	arg1	Levels					95:100	Increased N-Glycolylneuraminic Acid Levels	59:100	Increased N-Glycolylneuraminic Acid Levels	59:100	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	7	25	dep	lower	1040:1044	arg1	i					1047:1047	i	1047:1047	i	1047:1047	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	7	26	theme	Arctic	1190:1195	arg1	charr					1197:1201	control Arctic charr	1182:1201	control Arctic charr	1182:1201	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	9	27	theme	charr	1338:1342	arg1	repertoire					1351:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire differed from that of Atlantic salmon.					
30848132	4	28	from	full-fat	555:562	arg1	mucins					531:536	mucins	531:536	mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr	531:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	4	29	theme	soy-bean-fed	573:584	arg1	charr					620:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	10	30	theme	Arctic	1493:1498	arg1	char					1500:1503	Arctic char	1493:1503	Arctic char	1493:1503	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	5	31	theme	sialylated	794:803	arg1	O-glycans					805:813	core-5-type and sialylated O-glycans	778:813	core-5-type and sialylated O-glycans	778:813	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	7	32	theme	glycans	1160:1166	arg1	number					1050:1055	lower (i) number	1040:1055	lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans	1040:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	2	33	gly	glycosylated	347:358	arg1	proteins					360:367	highly glycosylated proteins	340:367	highly glycosylated proteins called mucins	340:381	The intestinal tract is covered by a mucus layer mainly comprised by highly glycosylated proteins called mucins.					
30848132	10	34	theme	inflammation-induced	1510:1529	arg1	changes					1545:1551	inflammation-induced glycosylation changes	1510:1551	inflammation-induced glycosylation changes	1510:1551	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	5	35	gly	sialylated	794:803	arg1	O-glycans					805:813	core-5-type and sialylated O-glycans	778:813	core-5-type and sialylated O-glycans	778:813	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	6	36	theme	NeuAc	886:890	arg1	α2,8NeuGc					895:903	NeuAc(Gc)α2,8NeuGc	886:903	NeuAc(Gc)α2,8NeuGc(Ac)	886:907	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	36	theme	NeuAc	886:890	arg1	Ac					905:906	Ac	905:906	Ac	905:906	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	0	37	from	Response	144:151	arg1	Evidence					47:54	Evidence	47:54	Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation	47:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	1	38	theme	aquaculture	249:259	arg1	industry					261:268	the aquaculture industry	245:268	the aquaculture industry	245:268	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	0	39	dep	Exploring	0:8	arg1	Evidence					47:54	Evidence	47:54	Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation	47:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	0	40	from	Interactions	128:139	arg1	Response					144:151	Response	144:151	Response to Inflammation	144:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	10	41	theme	host-pathogen	1560:1572	arg1	interactions					1574:1585	host-pathogen interactions	1560:1585	host-pathogen interactions	1560:1585	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	7	42	theme	soy	1017:1019	arg1	diet					1031:1034	soy bean meal diet	1017:1034	soy bean meal diet	1017:1034	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	7	43	theme	variation	1102:1110	arg1	number					1050:1055	lower (i) number	1040:1055	lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans	1040:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	6	44	theme	Disialic-acid-epitope-containing	816:847	arg1	structures					849:858	Disialic-acid-epitope-containing structures	816:858	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc	816:927	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	44	theme	Disialic-acid-epitope-containing	816:847	arg1	α2,8NeuGc					895:903	NeuAc(Gc)α2,8NeuGc	886:903	NeuAc(Gc)α2,8NeuGc(Ac)	886:907	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	44	theme	Disialic-acid-epitope-containing	816:847	arg1	NeuGcα2,8NeuGc					914:927	NeuGcα2,8NeuGc	914:927	NeuGcα2,8NeuGc	914:927	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	44	theme	Disialic-acid-epitope-containing	816:847	arg1	hallmark					938:945	the hallmark	934:945	the hallmark of Arctic charr intestinal mucin glycosylation	934:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	6	44	theme	Disialic-acid-epitope-containing	816:847	arg1	NeuAcα2,8NeuAc					870:883	NeuAcα2,8NeuAc	870:883	NeuAcα2,8NeuAc	870:883	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	4	45	from	control	543:549	arg1	mucins					531:536	mucins	531:536	mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr	531:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	7	46	dep	variation	1102:1110	arg1	ii					1082:1083	ii	1082:1083	ii	1082:1083	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	5	47	theme	core-5-type	778:788	arg1	O-glycans					805:813	core-5-type and sialylated O-glycans	778:813	core-5-type and sialylated O-glycans	778:813	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	1	48	theme	limiting	194:201	arg1	factor					203:208	a limiting factor	192:208	a limiting factor for the sustainable development of the aquaculture industry	192:268	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	1	48	theme	limiting	194:201	arg1	outbreaks					178:186	Disease outbreaks	170:186	Disease outbreaks	170:186	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	4	49	theme	liquid	632:637	arg1	spectrometry					666:677	liquid chromatography-tandem mass spectrometry	632:677	liquid chromatography-tandem mass spectrometry	632:677	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	5	50	theme	charr	727:731	arg1	mucins					744:749	Arctic charr intestinal mucins	720:749	Arctic charr intestinal mucins	720:749	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	1	51	theme	sustainable	218:228	arg1	development					230:240	the sustainable development	214:240	the sustainable development of the aquaculture industry	214:268	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	8	52	theme	Aeromonas	1217:1225	arg1	salmonicida					1227:1237	Aeromonas salmonicida	1217:1237	Aeromonas salmonicida	1217:1237	Furthermore, Aeromonas salmonicida grew less in response to mucins from inflamed Arctic charr than from the control group.					
30848132	10	53	from	biomarker	1463:1471	arg1	conclusion					1404:1413	conclusion	1404:1413	conclusion	1404:1413	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	4	54	theme	mass	661:664	arg1	spectrometry					666:677	liquid chromatography-tandem mass spectrometry	632:677	liquid chromatography-tandem mass spectrometry	632:677	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	7	55	theme	lower	1040:1044	arg1	number					1050:1055	lower (i) number	1040:1055	lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans	1040:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	10	56	from	inflammation	1477:1488	arg1	char					1500:1503	Arctic char	1493:1503	Arctic char	1493:1503	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	4	57	dep	charr	620:624	arg1	known					587:591	known	587:591	known to cause enteritis	587:610	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	5	58	theme	high	759:762	arg1	prevalence					764:773	a high prevalence	757:773	a high prevalence of core-5-type and sialylated O-glycans	757:813	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	4	59	theme	chromatography-tandem	639:659	arg1	spectrometry					666:677	liquid chromatography-tandem mass spectrometry	632:677	liquid chromatography-tandem mass spectrometry	632:677	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	4	60	theme	mucin	512:516	arg1	O-glycans					518:526	intestinal mucin O-glycans	501:526	intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr	501:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	0	61	theme	Acid	90:93	arg1	Levels					95:100	Increased N-Glycolylneuraminic Acid Levels	59:100	Increased N-Glycolylneuraminic Acid Levels	59:100	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	4	62	dep	control	543:549	arg1	charr					620:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	1	63	theme	Disease	170:176	arg1	factor					203:208	a limiting factor	192:208	a limiting factor for the sustainable development of the aquaculture industry	192:268	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	1	63	theme	Disease	170:176	arg1	outbreaks					178:186	Disease outbreaks	170:186	Disease outbreaks	170:186	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
30848132	0	64	theme	Interactions	128:139	arg1	Evidence					47:54	Evidence	47:54	Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation	47:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	0	65	from	Evidence	47:54	arg1	Response					144:151	Response	144:151	Response to Inflammation	144:167	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	10	66	theme	N-glycolylneuraminic	1428:1447	arg1	acid					1449:1452	N-glycolylneuraminic acid	1428:1452	N-glycolylneuraminic acid	1428:1452	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	0	67	theme	Host-Pathogen	114:126	arg1	Interactions					128:139	Changed Host-Pathogen Interactions	106:139	Changed Host-Pathogen Interactions	106:139	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	7	68	theme	bean	1021:1024	arg1	diet					1031:1034	soy bean meal diet	1017:1034	soy bean meal diet	1017:1034	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	7	69	theme	control	1182:1188	arg1	charr					1197:1201	control Arctic charr	1182:1201	control Arctic charr	1182:1201	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	9	70	theme	Arctic	1331:1336	arg1	repertoire					1351:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire differed from that of Atlantic salmon.					
30848132	4	71	theme	extruded	564:571	arg1	charr					620:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	7	72	dep	glycans	1160:1166	arg1	iii					1118:1120	iii	1118:1120	iii	1118:1120	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	0	73	theme	Charr	21:25	arg1	Glycome					38:44	the Arctic Charr Intestinal Glycome	10:44	the Arctic Charr Intestinal Glycome	10:44	Exploring the Arctic Charr Intestinal Glycome: Evidence of Increased N-Glycolylneuraminic Acid Levels and Changed Host-Pathogen Interactions in Response to Inflammation.					
30848132	9	74	theme	glycan	1344:1349	arg1	repertoire					1351:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire	1327:1360	The Arctic charr glycan repertoire differed from that of Atlantic salmon.					
30848132	3	75	theme	pathogen	400:407	arg1	adhesion					409:416	pathogen adhesion	400:416	pathogen adhesion	400:416	Mucins regulate pathogen adhesion, growth, and virulence, and the glycans are vital for these functions.					
30848132	7	76	theme	N-glycolylneuraminic-acid-containing	1123:1158	arg1	glycans					1160:1166	N-glycolylneuraminic-acid-containing glycans	1123:1166	(iii) N-glycolylneuraminic-acid-containing glycans	1117:1166	Arctic charr fed with soy bean meal diet had lower (i) number of structures detected, (ii) interindividual variation, and (iii) N-glycolylneuraminic-acid-containing glycans compared with control Arctic charr.					
30848132	5	77	theme	O-glycans	805:813	arg1	prevalence					764:773	a high prevalence	757:773	a high prevalence of core-5-type and sialylated O-glycans	757:813	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	6	78	theme	mucin	974:978	arg1	glycosylation					980:992	Arctic charr intestinal mucin glycosylation	950:992	Arctic charr intestinal mucin glycosylation	950:992	Disialic-acid-epitope-containing structures including NeuAcα2,8NeuAc, NeuAc(Gc)α2,8NeuGc(Ac), and NeuGcα2,8NeuGc were the hallmark of Arctic charr intestinal mucin glycosylation.					
30848132	5	79	theme	intestinal	733:742	arg1	mucins					744:749	Arctic charr intestinal mucins	720:749	Arctic charr intestinal mucins	720:749	In total, 56 glycans were identified on Arctic charr intestinal mucins, with a high prevalence of core-5-type and sialylated O-glycans.					
30848132	10	80	theme	glycosylation	1531:1543	arg1	changes					1545:1551	inflammation-induced glycosylation changes	1510:1551	inflammation-induced glycosylation changes	1510:1551	In conclusion, the loss of N-glycolylneuraminic acid may be a biomarker for inflammation in Arctic char, and inflammation-induced glycosylation changes affect host-pathogen interactions.					
30848132	2	81	theme	glycosylated	347:358	arg1	proteins					360:367	highly glycosylated proteins	340:367	highly glycosylated proteins called mucins	340:381	The intestinal tract is covered by a mucus layer mainly comprised by highly glycosylated proteins called mucins.					
30848132	9	82	theme	Atlantic	1384:1391	arg1	salmon					1393:1398	Atlantic salmon	1384:1398	Atlantic salmon	1384:1398	The Arctic charr glycan repertoire differed from that of Atlantic salmon.					
30848132	4	83	theme	Arctic	613:618	arg1	charr					620:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	extruded soy-bean-fed (known to cause enteritis) Arctic charr	564:624	We analyzed intestinal mucin O-glycans on mucins from control and full-fat extruded soy-bean-fed (known to cause enteritis) Arctic charr using liquid chromatography-tandem mass spectrometry.					
30848132	8	84	theme	Arctic	1285:1290	arg1	charr					1292:1296	inflamed Arctic charr	1276:1296	inflamed Arctic charr	1276:1296	Furthermore, Aeromonas salmonicida grew less in response to mucins from inflamed Arctic charr than from the control group.					
30848132	1	85	theme	industry	261:268	arg1	development					230:240	the sustainable development	214:240	the sustainable development of the aquaculture industry	214:268	Disease outbreaks are a limiting factor for the sustainable development of the aquaculture industry.					
29991969	9	0	theme	glycosylation	1804:1816	arg1	genes					1818:1822	key glycosylation genes	1800:1822	key glycosylation genes	1800:1822	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	6	1	theme	promoter	1204:1211	arg1	methylation					1213:1223	the MGAT3 and BACH2 promoter methylation	1184:1223	methylation	1213:1223	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	8	2	theme	IgG	1604:1606	arg1	glycans					1608:1614	IgG glycans	1604:1614	IgG glycans	1604:1614	The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.					
29991969	1	3	theme	promoter	239:246	arg1	methylation					248:258	promoter methylation	239:258	promoter methylation of candidate genes for inflammatory bowel disease (IBD)	239:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	4	4	theme	significant	837:847	arg1	differences					849:859	significant differences	837:859	significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis	837:966	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	3	5	theme	genes	689:693	arg1	regions					667:673	promoter regions	658:673	promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	658:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	10	6	attach	isolated	2125:2132	arg2	cells					2119:2123	CD3+ T cells	2112:2123	CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available	2112:2252	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	10	6	attach	isolated	2125:2132	arg1	cohort					2212:2217	a third smaller cohort	2196:2217	a third smaller cohort	2196:2217	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	10	6	attach	isolated	2125:2132	arg1	mucosa					2148:2153	inflamed mucosa	2139:2153	inflamed mucosa of patients with ulcerative colitis	2139:2189	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	7	7	theme	bisecting	1403:1411	arg1	GlcNAc					1413:1418	bisecting GlcNAc	1403:1418	bisecting GlcNAc	1403:1418	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	10	8	theme	CpG	2048:2050	arg1	methylation					2052:2062	CpG methylation	2048:2062	CpG methylation in the promoter of the MGAT3 gene	2048:2096	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	10	9	theme	ulcerative	2172:2181	arg1	colitis					2183:2189	ulcerative colitis	2172:2189	ulcerative colitis	2172:2189	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	7	10	from	sialylation	1386:1396	arg1	IgG					1423:1425	IgG	1423:1425	IgG of the same patients	1423:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	10	11	theme	smaller	2204:2210	arg1	cohort					2212:2217	a third smaller cohort	2196:2217	a third smaller cohort	2196:2217	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	3	12	from	cohorts	797:803	arg1	HCs					771:773	HCs	771:773	HCs	771:773	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	12	from	cohorts	797:803	arg1	patients					740:747	hundred IBD patients	728:747	hundred IBD patients	728:747	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	12	from	cohorts	797:803	arg1	blood					711:715	peripheral blood	700:715	peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	700:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	12	from	cohorts	797:803	arg1	controls					761:768	healthy controls	753:768	healthy controls (HCs)	753:774	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	1	13	theme	association	188:198	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	7	14	theme	MGAT3	1312:1316	arg1	methylation					1327:1337	MGAT3 promoter methylation	1312:1337	MGAT3 promoter methylation	1312:1337	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	9	15	theme	pro-inflammatory	1853:1868	arg1	properties					1870:1879	pro-inflammatory properties	1853:1879	pro-inflammatory properties of IgG	1853:1886	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	3	16	theme	peripheral	700:709	arg1	blood					711:715	peripheral blood	700:715	peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	700:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	0	17	theme	glycome	106:112	arg1	composition					70:80	the composition	66:80	the composition of the immunoglobulin G glycome in inflammatory bowel disease	66:142	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	5	18	theme	methylation	1009:1019	arg1	changes					1021:1027	methylation changes	1009:1027	methylation changes	1009:1027	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	7	19	from	GlcNAc	1413:1418	arg1	IgG					1423:1425	IgG	1423:1425	IgG of the same patients	1423:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	4	20	theme	MGAT3	894:898	arg1	genes					910:914	the MGAT3 and BACH2 genes	890:914	the MGAT3 and BACH2 genes	890:914	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	3	21	theme	patients	740:747	arg1	blood					711:715	peripheral blood	700:715	peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	700:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	5	22	theme	IBD	1125:1127	arg1	patients					1129:1136	the IBD patients	1121:1136	the IBD patients	1121:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	6	23	theme	correlation	1141:1151	arg1	analysis					1153:1160	A correlation analysis	1139:1160	A correlation analysis	1139:1160	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	10	24	theme	gene	2093:2096	arg1	promoter					2071:2078	the promoter	2067:2078	the promoter of the MGAT3 gene	2067:2096	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	6	25	theme	IgG	1240:1242	arg1	glycans					1244:1250	individual IgG glycans	1229:1250	individual IgG glycans	1229:1250	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	4	26	theme	methylation	868:878	arg1	levels					880:885	the methylation levels	864:885	the methylation levels in the MGAT3 and BACH2 genes	864:914	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	5	27	theme	patients	1129:1136	arg1	subset					1111:1116	a subset	1109:1116	a subset of the IBD patients	1109:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	10	28	theme	CD3+	2112:2115	arg1	cells					2119:2123	CD3+ T cells	2112:2123	CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available	2112:2252	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	3	29	theme	healthy	753:759	arg1	HCs					771:773	HCs	771:773	HCs	771:773	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	29	theme	healthy	753:759	arg1	controls					761:768	healthy controls	753:768	healthy controls (HCs)	753:774	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	6	30	theme	cohorts	1303:1309	arg1	individuals					1274:1284	the same individuals	1265:1284	the same individuals of the two large cohorts	1265:1309	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	5	31	theme	CD19+	1062:1066	arg1	cells					1070:1074	CD19+ B cells	1062:1074	CD19+ B cells isolated from the whole blood of a subset of the IBD patients	1062:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	8	32	theme	IgG	1701:1703	arg1	glycosylation					1705:1717	IgG glycosylation	1701:1717	IgG glycosylation	1701:1717	The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.					
29991969	1	33	theme	Many	156:159	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	7	34	from	galactosylation	1369:1383	arg1	IgG					1423:1425	IgG	1423:1425	IgG of the same patients	1423:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	2	35	theme	MGAT3	503:507	arg1	loci					509:512	MGAT3 loci	503:512	MGAT3 loci	503:512	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	9	36	from	structures	2012:2021	arg1	decrease					1905:1912	a decrease	1903:1912	a decrease in galactosylation and sialylation	1903:1947	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	36	from	structures	2012:2021	arg1	increase					1956:1963	an increase	1953:1963	an increase of bisecting GlcNAc on digalactosylated glycan structures	1953:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	2	37	dep	BACH2	471:475	arg1	the					467:469	the	467:469	the	467:469	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	7	38	theme	GnT-III	1481:1487	arg1	enzyme					1489:1494	the GnT-III enzyme	1477:1494	the GnT-III enzyme	1477:1494	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	0	39	theme	immunoglobulin	89:102	arg1	glycome					106:112	the immunoglobulin G glycome	85:112	the immunoglobulin G glycome	85:112	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	1	40	theme	genetic	367:373	arg1	changes					390:396	genetic and epigenetic changes	367:396	genetic and epigenetic changes	367:396	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	10	41	theme	patients	2158:2165	arg1	mucosa					2148:2153	inflamed mucosa	2139:2153	inflamed mucosa of patients with ulcerative colitis	2139:2189	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	0	42	theme	Promoter	0:7	arg1	methylation					9:19	Promoter methylation	0:19	Promoter methylation of the MGAT3 and BACH2 genes	0:48	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	1	43	theme	candidate	263:271	arg1	genes					273:277	candidate genes	263:277	candidate genes for inflammatory bowel disease (IBD)	263:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	9	44	from	decrease	1905:1912	arg1	structures					2012:2021	digalactosylated glycan structures	1988:2021	digalactosylated glycan structures	1988:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	44	from	decrease	1905:1912	arg1	sialylation					1937:1947	sialylation	1937:1947	sialylation	1937:1947	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	44	from	decrease	1905:1912	arg1	galactosylation					1917:1931	galactosylation	1917:1931	galactosylation	1917:1931	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	45	theme	GlcNAc	1978:1983	arg1	decrease					1905:1912	a decrease	1903:1912	a decrease in galactosylation and sialylation	1903:1947	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	45	theme	GlcNAc	1978:1983	arg1	increase					1956:1963	an increase	1953:1963	an increase of bisecting GlcNAc on digalactosylated glycan structures	1953:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	5	46	theme	subset	1111:1116	arg1	blood					1100:1104	the whole blood	1090:1104	the whole blood of a subset of the IBD patients	1090:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	4	47	theme	ulcerative	949:958	arg1	colitis					960:966	ulcerative colitis	949:966	ulcerative colitis	949:966	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	9	48	theme	digalactosylated	1988:2003	arg1	structures					2012:2021	digalactosylated glycan structures	1988:2021	digalactosylated glycan structures	1988:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	2	49	theme	Independent	407:417	arg1	studies					423:429	Independent GWA studies	407:429	Independent GWA studies	407:429	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	0	50	theme	MGAT3	28:32	arg1	genes					44:48	the MGAT3 and BACH2 genes	24:48	genes	44:48	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	1	51	theme	bowel	296:300	arg1	disease					302:308	inflammatory bowel disease	283:308	inflammatory bowel disease (IBD)	283:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	1	51	theme	bowel	296:300	arg1	IBD					311:313	IBD	311:313	IBD	311:313	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	3	52	theme	bisulfite	601:609	arg1	pyrosequencing					611:624	bisulfite pyrosequencing	601:624	bisulfite pyrosequencing	601:624	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	10	53	from	methylation	2052:2062	arg1	promoter					2071:2078	the promoter	2067:2078	the promoter of the MGAT3 gene	2067:2096	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	0	54	theme	BACH2	38:42	arg1	genes					44:48	the MGAT3 and BACH2 genes	24:48	genes	44:48	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	5	55	theme	whole	1094:1098	arg1	blood					1100:1104	the whole blood	1090:1104	the whole blood of a subset of the IBD patients	1090:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	7	56	theme	patients	1439:1446	arg1	IgG					1423:1425	IgG	1423:1425	IgG of the same patients	1423:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	9	57	from	GlcNAc	1978:1983	arg1	structures					2012:2021	digalactosylated glycan structures	1988:2021	digalactosylated glycan structures	1988:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	1	58	dep	studies	200:206	arg1	EWAS					218:221	EWAS	218:221	EWAS	218:221	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	1	58	dep	studies	200:206	arg1	GWAS					209:212	GWAS	209:212	GWAS	209:212	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	9	59	theme	key	1800:1802	arg1	genes					1818:1822	key glycosylation genes	1800:1822	key glycosylation genes	1800:1822	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	8	60	theme	promoter	1579:1586	arg1	methylation					1588:1598	the BACH2 promoter methylation	1569:1598	the BACH2 promoter methylation	1569:1598	The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.					
29991969	2	61	dep	IBD	541:543	arg1	glycosylation					572:584	glycosylation	572:584	glycosylation	572:584	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	9	62	theme	genes	1818:1822	arg1	deregulation					1784:1795	epigenetic deregulation	1773:1795	epigenetic deregulation of key glycosylation genes	1773:1822	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	3	63	theme	promoter	658:665	arg1	regions					667:673	promoter regions	658:673	promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	658:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	6	64	theme	BACH2	1198:1202	arg1	methylation					1213:1223	the MGAT3 and BACH2 promoter methylation	1184:1223	methylation	1213:1223	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	1	65	theme	methylation	248:258	arg1	studies					228:234	studies	228:234	studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD)	228:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	1	65	theme	methylation	248:258	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	10	66	with	patients	2158:2165	arg1	colitis					2183:2189	ulcerative colitis	2172:2189	ulcerative colitis	2172:2189	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	2	67	theme	immunoglobulin	549:562	arg1	IgG					567:569	IgG	567:569	IgG	567:569	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	2	67	theme	immunoglobulin	549:562	arg1	G					564:564	immunoglobulin G	549:564	immunoglobulin G (IgG)	549:570	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	1	68	theme	genome-	161:167	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	4	69	dep	Results	820:826	arg1	found					831:835	found	831:835	found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC	831:986	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	9	70	dep	Conclusions	1736:1746	arg1	suggest					1760:1766	suggest	1760:1766	suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures	1760:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	1	71	theme	epigenome-wide	173:186	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	3	72	theme	several	720:726	arg1	patients					740:747	hundred IBD patients	728:747	hundred IBD patients	728:747	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	0	73	from	composition	70:80	arg1	disease					136:142	inflammatory bowel disease	117:142	inflammatory bowel disease	117:142	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	7	74	theme	promoter	1318:1325	arg1	methylation					1327:1337	MGAT3 promoter methylation	1312:1337	MGAT3 promoter methylation	1312:1337	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	0	75	theme	bowel	130:134	arg1	disease					136:142	inflammatory bowel disease	117:142	inflammatory bowel disease	117:142	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	5	76	theme	changes	1021:1027	arg1	pattern					998:1004	The same pattern	989:1004	The same pattern of methylation changes	989:1027	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	10	77	from	role	2279:2282	arg1	pathogenesis					2310:2321	IBD pathogenesis	2306:2321	IBD pathogenesis	2306:2321	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	3	78	theme	independent	785:795	arg1	cohorts					797:803	two independent cohorts	781:803	two independent cohorts	781:803	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	9	79	theme	IgG	1884:1886	arg1	properties					1870:1879	pro-inflammatory properties	1853:1879	pro-inflammatory properties of IgG	1853:1886	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	3	80	from	regions	667:673	arg1	blood					711:715	peripheral blood	700:715	peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	700:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	4	81	theme	BACH2	904:908	arg1	genes					910:914	the MGAT3 and BACH2 genes	890:914	the MGAT3 and BACH2 genes	890:914	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	4	82	from	levels	880:885	arg1	genes					910:914	the MGAT3 and BACH2 genes	890:914	the MGAT3 and BACH2 genes	890:914	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	10	83	theme	MGAT3	2087:2091	arg1	gene					2093:2096	the MGAT3 gene	2083:2096	the MGAT3 gene	2083:2096	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	3	84	theme	IBD	736:738	arg1	patients					740:747	hundred IBD patients	728:747	hundred IBD patients	728:747	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	6	85	theme	individual	1229:1238	arg1	glycans					1244:1250	individual IgG glycans	1229:1250	individual IgG glycans	1229:1250	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	5	86	theme	same	993:996	arg1	pattern					998:1004	The same pattern	989:1004	The same pattern of methylation changes	989:1027	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	9	87	from	increase	1841:1848	arg1	IBD					1891:1893	IBD	1891:1893	IBD	1891:1893	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	87	from	increase	1841:1848	arg1	properties					1870:1879	pro-inflammatory properties	1853:1879	pro-inflammatory properties of IgG	1853:1886	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	10	88	theme	T	2117:2117	arg1	cells					2119:2123	CD3+ T cells	2112:2123	CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available	2112:2252	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	9	89	from	increase	1956:1963	arg1	structures					2012:2021	digalactosylated glycan structures	1988:2021	digalactosylated glycan structures	1988:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	89	from	increase	1956:1963	arg1	sialylation					1937:1947	sialylation	1937:1947	sialylation	1937:1947	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	9	89	from	increase	1956:1963	arg1	galactosylation					1917:1931	galactosylation	1917:1931	galactosylation	1917:1931	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	4	90	from	differences	849:859	arg1	levels					880:885	the methylation levels	864:885	the methylation levels in the MGAT3 and BACH2 genes	864:914	Results We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.					
29991969	3	91	theme	controls	761:768	arg1	blood					711:715	peripheral blood	700:715	peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	700:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	6	92	theme	large	1297:1301	arg1	cohorts					1303:1309	the two large cohorts	1289:1309	the two large cohorts	1289:1309	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	5	93	theme	B	1068:1068	arg1	cells					1070:1074	CD19+ B cells	1062:1074	CD19+ B cells isolated from the whole blood of a subset of the IBD patients	1062:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	1	94	theme	Background	145:154	arg1	studies					200:206	Background Many genome- and epigenome-wide association studies	145:206	Background Many genome- and epigenome-wide association studies (GWAS and EWAS)	145:222	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	7	95	theme	enzyme	1489:1494	arg1	activity					1465:1472	activity	1465:1472	activity	1465:1472	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	10	96	theme	functional	2268:2277	arg1	role					2279:2282	a functional role	2266:2282	a functional role of this glyco-gene in IBD pathogenesis	2266:2321	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	2	97	from	variants	455:462	arg1	LAMB1					485:489	LAMB1	485:489	LAMB1	485:489	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	2	97	from	variants	455:462	arg1	loci					509:512	MGAT3 loci	503:512	MGAT3 loci	503:512	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	2	97	from	variants	455:462	arg1	BACH2					471:475	BACH2	471:475	BACH2	471:475	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	2	97	from	variants	455:462	arg1	IL6ST					478:482	IL6ST	478:482	IL6ST	478:482	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	2	97	from	variants	455:462	arg1	IKZF1					492:496	IKZF1	492:496	IKZF1	492:496	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	10	98	theme	inflamed	2139:2146	arg1	mucosa					2148:2153	inflamed mucosa	2139:2153	inflamed mucosa of patients with ulcerative colitis	2139:2189	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	1	99	theme	epigenetic	379:388	arg1	changes					390:396	genetic and epigenetic changes	367:396	genetic and epigenetic changes	367:396	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	1	100	theme	significant	334:344	arg1	associations					346:357	significant associations	334:357	significant associations between genetic and epigenetic changes and IBD	334:404	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	10	101	theme	IBD	2306:2308	arg1	pathogenesis					2310:2321	IBD pathogenesis	2306:2321	IBD pathogenesis	2306:2321	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	0	102	theme	G	104:104	arg1	glycome					106:112	the immunoglobulin G glycome	85:112	the immunoglobulin G glycome	85:112	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	10	103	theme	glyco-gene	2292:2301	arg1	role					2279:2282	a functional role	2266:2282	a functional role of this glyco-gene in IBD pathogenesis	2266:2321	Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.					
29991969	3	104	from	blood	711:715	arg1	regions					667:673	promoter regions	658:673	promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	658:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	104	from	blood	711:715	arg1	cohorts					797:803	two independent cohorts	781:803	two independent cohorts	781:803	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	3	104	from	blood	711:715	arg1	genes					689:693	these five genes	678:693	these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively	678:817	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	5	105	from	genes	1053:1057	arg1	cells					1070:1074	CD19+ B cells	1062:1074	CD19+ B cells isolated from the whole blood of a subset of the IBD patients	1062:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	7	106	gly	sialylation	1386:1396	arg1	IgG					1423:1425	IgG	1423:1425	IgG of the same patients	1423:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
29991969	2	107	theme	genetic	447:453	arg1	variants					455:462	genetic variants	447:462	genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci	447:512	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	9	108	theme	bisecting	1968:1976	arg1	GlcNAc					1978:1983	bisecting GlcNAc	1968:1983	bisecting GlcNAc on digalactosylated glycan structures	1968:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	1	109	theme	genes	273:277	arg1	methylation					248:258	promoter methylation	239:258	promoter methylation of candidate genes for inflammatory bowel disease (IBD)	239:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	2	110	theme	GWA	419:421	arg1	studies					423:429	Independent GWA studies	407:429	Independent GWA studies	407:429	Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.					
29991969	1	111	theme	inflammatory	283:294	arg1	disease					302:308	inflammatory bowel disease	283:308	inflammatory bowel disease (IBD)	283:314	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	1	111	theme	inflammatory	283:294	arg1	IBD					311:313	IBD	311:313	IBD	311:313	Background Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.					
29991969	9	112	theme	glycan	2005:2010	arg1	structures					2012:2021	digalactosylated glycan structures	1988:2021	digalactosylated glycan structures	1988:2021	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	6	113	theme	same	1269:1272	arg1	individuals					1274:1284	the same individuals	1265:1284	the same individuals of the two large cohorts	1265:1309	A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.					
29991969	0	114	theme	genes	44:48	arg1	methylation					9:19	Promoter methylation	0:19	Promoter methylation of the MGAT3 and BACH2 genes	0:48	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	0	115	theme	inflammatory	117:128	arg1	disease					136:142	inflammatory bowel disease	117:142	inflammatory bowel disease	117:142	Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.					
29991969	3	116	theme	CpG	639:641	arg1	methylation					643:653	CpG methylation	639:653	CpG methylation	639:653	Methods Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.					
29991969	5	117	attach	isolated	1076:1083	arg1	blood					1100:1104	the whole blood	1090:1104	the whole blood of a subset of the IBD patients	1090:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	5	117	attach	isolated	1076:1083	arg2	cells					1070:1074	CD19+ B cells	1062:1074	CD19+ B cells isolated from the whole blood of a subset of the IBD patients	1062:1136	The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.					
29991969	9	118	theme	epigenetic	1773:1782	arg1	deregulation					1784:1795	epigenetic deregulation	1773:1795	epigenetic deregulation of key glycosylation genes	1773:1822	Conclusions Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.					
29991969	8	119	theme	BACH2	1573:1577	arg1	methylation					1588:1598	the BACH2 promoter methylation	1569:1598	the BACH2 promoter methylation	1569:1598	The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.					
29991969	7	120	theme	same	1434:1437	arg1	patients					1439:1446	the same patients	1430:1446	the same patients	1430:1446	MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.					
31717732	8	0	theme	cell	1028:1031	arg1	hTERT/HPNE					1039:1048	hTERT/HPNE	1039:1048	hTERT/HPNE	1039:1048	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	0	theme	cell	1028:1031	arg1	line					1033:1036	noncancerous pancreatic cell line	1004:1036	noncancerous pancreatic cell line (hTERT/HPNE)	1004:1049	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	1	1	theme	cellular	145:152	arg1	membrane					154:161	the outer cellular membrane	135:161	the outer cellular membrane	135:161	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	8	2	theme	pancreatic	1017:1026	arg1	hTERT/HPNE					1039:1048	hTERT/HPNE	1039:1048	hTERT/HPNE	1039:1048	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	2	theme	pancreatic	1017:1026	arg1	line					1033:1036	noncancerous pancreatic cell line	1004:1036	noncancerous pancreatic cell line (hTERT/HPNE)	1004:1049	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	3	3	from	profiling	393:401	arg1	NCI-H358					499:506	NCI-H358	499:506	NCI-H358	499:506	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	3	from	profiling	393:401	arg1	lines					469:473	various cell lines	456:473	various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski	456:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	3	from	profiling	393:401	arg1	CFPAC1					485:490	CFPAC1	485:490	CFPAC1	485:490	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	3	from	profiling	393:401	arg1	MCF7					509:512	MCF7	509:512	MCF7	509:512	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	3	from	profiling	393:401	arg1	Caski					519:523	Caski	519:523	Caski	519:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	3	from	profiling	393:401	arg1	A549					493:496	A549	493:496	A549	493:496	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	7	4	theme	ceramide	972:979	arg1	moieties					981:988	ceramide moieties	972:988	ceramide moieties	972:988	Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties.					
31717732	8	5	theme	cancerous	1147:1155	arg1	CFPAC1					1179:1184	CFPAC1	1179:1184	CFPAC1	1179:1184	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	5	theme	cancerous	1147:1155	arg1	line					1173:1176	cancerous pancreatic cell line	1147:1176	cancerous pancreatic cell line (CFPAC1)	1147:1185	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	9	6	theme	subgrouping	1351:1361	arg1	types					1375:1379	subgrouping cancer cell types	1351:1379	subgrouping cancer cell types	1351:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	4	7	from	total	557:561	arg1	membrane					590:597	cell membrane	585:597	cell membrane	585:597	We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS.					
31717732	2	8	theme	effective	334:342	arg1	tools					355:359	effective analytical tools	334:359	effective analytical tools	334:359	Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools.					
31717732	9	9	theme	cancer	1363:1368	arg1	types					1375:1379	subgrouping cancer cell types	1351:1379	subgrouping cancer cell types	1351:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	3	10	theme	cell	464:467	arg1	NCI-H358					499:506	NCI-H358	499:506	NCI-H358	499:506	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	10	theme	cell	464:467	arg1	lines					469:473	various cell lines	456:473	various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski	456:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	10	theme	cell	464:467	arg1	CFPAC1					485:490	CFPAC1	485:490	CFPAC1	485:490	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	10	theme	cell	464:467	arg1	MCF7					509:512	MCF7	509:512	MCF7	509:512	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	10	theme	cell	464:467	arg1	Caski					519:523	Caski	519:523	Caski	519:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	10	theme	cell	464:467	arg1	A549					493:496	A549	493:496	A549	493:496	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	1	11	theme	key	172:174	arg1	marker					125:130	a surface marker	115:130	a surface marker	115:130	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	1	11	theme	key	172:174	arg1	Gangliosides					95:106	Gangliosides	95:106	Gangliosides	95:106	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	1	11	theme	key	172:174	arg1	roles					176:180	key roles	172:180	key roles	172:180	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	8	12	theme	pancreatic	1157:1166	arg1	CFPAC1					1179:1184	CFPAC1	1179:1184	CFPAC1	1179:1184	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	12	theme	pancreatic	1157:1166	arg1	line					1173:1176	cancerous pancreatic cell line	1147:1176	cancerous pancreatic cell line (CFPAC1)	1147:1185	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	2	13	theme	gangliosides	259:270	arg1	importance					245:254	the biological importance	230:254	the biological importance of gangliosides	230:270	Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools.					
31717732	3	14	theme	intact	433:438	arg1	gangliosides					440:451	intact gangliosides	433:451	intact gangliosides	433:451	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	8	15	theme	different	1101:1109	arg1	levels					1111:1116	different levels	1101:1116	different levels of sialic acid	1101:1131	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	9	16	theme	analytical	1246:1255	arg1	platform					1257:1264	our analytical platform	1242:1264	our analytical platform	1242:1264	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	7	17	theme	cell	956:959	arg1	lines					961:965	cancer cell lines	949:965	cancer cell lines	949:965	Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties.					
31717732	3	18	theme	structural	407:416	arg1	elucidation					418:428	structural elucidation	407:428	structural elucidation	407:428	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	0	19	theme	Comprehensive	0:12	arg1	Profiling					14:22	Comprehensive Profiling	0:22	Comprehensive Profiling of Surface Gangliosides	0:46	Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS.					
31717732	8	20	theme	noncancerous	1004:1015	arg1	hTERT/HPNE					1039:1048	hTERT/HPNE	1039:1048	hTERT/HPNE	1039:1048	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	20	theme	noncancerous	1004:1015	arg1	line					1033:1036	noncancerous pancreatic cell line	1004:1036	noncancerous pancreatic cell line (hTERT/HPNE)	1004:1049	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	9	21	theme	cell	1294:1297	arg1	gangliosides					1307:1318	cell surface gangliosides	1294:1318	cell surface gangliosides	1294:1318	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	8	22	theme	cell	1168:1171	arg1	CFPAC1					1179:1184	CFPAC1	1179:1184	CFPAC1	1179:1184	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	22	theme	cell	1168:1171	arg1	line					1173:1176	cancerous pancreatic cell line	1147:1176	cancerous pancreatic cell line (CFPAC1)	1147:1185	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	0	23	theme	Surface	27:33	arg1	Gangliosides					35:46	Surface Gangliosides	27:46	Surface Gangliosides	27:46	Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS.					
31717732	8	24	contain	containing	1090:1099	arg2	levels					1111:1116	different levels	1101:1116	different levels of sialic acid	1101:1131	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	8	24	contain	containing	1090:1099	arg1	gangliosides					1077:1088	gangliosides	1077:1088	gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1)	1077:1185	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	5	25	theme	high	666:669	arg1	complexity					671:680	high complexity	666:680	high complexity	666:680	Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively.					
31717732	9	26	theme	cell	1370:1373	arg1	types					1375:1379	subgrouping cancer cell types	1351:1379	subgrouping cancer cell types	1351:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	3	27	theme	various	456:462	arg1	NCI-H358					499:506	NCI-H358	499:506	NCI-H358	499:506	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	27	theme	various	456:462	arg1	lines					469:473	various cell lines	456:473	various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski	456:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	27	theme	various	456:462	arg1	CFPAC1					485:490	CFPAC1	485:490	CFPAC1	485:490	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	27	theme	various	456:462	arg1	MCF7					509:512	MCF7	509:512	MCF7	509:512	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	27	theme	various	456:462	arg1	Caski					519:523	Caski	519:523	Caski	519:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	27	theme	various	456:462	arg1	A549					493:496	A549	493:496	A549	493:496	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	1	28	theme	cancer	185:190	arg1	invasion					197:204	cancer cell invasion	185:204	cancer cell invasion	185:204	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	8	29	theme	acid	1128:1131	arg1	levels					1111:1116	different levels	1101:1116	different levels of sialic acid	1101:1131	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	9	30	theme	comprehensive	1269:1281	arg1	profile					1283:1289	comprehensive profile	1269:1289	comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types	1269:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	1	31	theme	cell	192:195	arg1	invasion					197:204	cancer cell invasion	185:204	cancer cell invasion	185:204	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	3	32	theme	gangliosides	440:451	arg1	profiling					393:401	molecular profiling	383:401	molecular profiling	383:401	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	32	theme	gangliosides	440:451	arg1	elucidation					418:428	structural elucidation	407:428	structural elucidation	407:428	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	0	33	theme	Gangliosides	35:46	arg1	Profiling					14:22	Comprehensive Profiling	0:22	Comprehensive Profiling of Surface Gangliosides	0:46	Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS.					
31717732	8	34	theme	sialic	1121:1126	arg1	acid					1128:1131	sialic acid	1121:1131	sialic acid	1121:1131	In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1).					
31717732	6	35	theme	abundant	744:751	arg1	GM3					765:767	GM3	765:767	GM3(d34:1)	765:774	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	6	35	theme	abundant	744:751	arg1	species					753:759	The most abundant species	735:759	The most abundant species	735:759	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	4	36	theme	C18	605:607	arg1	LC-MS/MS					609:616	C18 LC-MS/MS	605:616	C18 LC-MS/MS	605:616	We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS.					
31717732	0	37	theme	Various	63:69	arg1	Lines					76:80	Various Cell Lines	63:80	Various Cell Lines	63:80	Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS.					
31717732	7	38	theme	cancer	949:954	arg1	lines					961:965	cancer cell lines	949:965	cancer cell lines	949:965	Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties.					
31717732	9	39	theme	platform	1257:1264	arg1	feasibility					1227:1237	the feasibility	1223:1237	the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types	1223:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	7	40	theme	more	926:929	arg1	diversity					931:939	more diversity	926:939	more diversity between cancer cell lines than ceramide moieties	926:988	Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties.					
31717732	3	41	theme	molecular	383:391	arg1	profiling					393:401	molecular profiling	383:401	molecular profiling	383:401	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	6	42	theme	major	844:848	arg1	components					850:859	major components	844:859	major components	844:859	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	5	43	theme	cell	646:649	arg1	line					651:654	each cell line	641:654	each cell line	641:654	Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively.					
31717732	7	44	theme	glycan	903:908	arg1	moieties					910:917	glycan moieties	903:917	glycan moieties	903:917	Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties.					
31717732	1	45	theme	surface	117:123	arg1	marker					125:130	a surface marker	115:130	a surface marker	115:130	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	1	45	theme	surface	117:123	arg1	Gangliosides					95:106	Gangliosides	95:106	Gangliosides	95:106	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	1	45	theme	surface	117:123	arg1	roles					176:180	key roles	172:180	key roles	172:180	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	9	46	theme	surface	1299:1305	arg1	gangliosides					1307:1318	cell surface gangliosides	1294:1318	cell surface gangliosides	1294:1318	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	9	47	theme	gangliosides	1307:1318	arg1	profile					1283:1289	comprehensive profile	1269:1289	comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types	1269:1379	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
31717732	4	48	theme	gangliosides	569:580	arg1	total					557:561	a total	555:561	a total of 76 gangliosides on cell membrane	555:597	We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS.					
31717732	6	49	from	components	850:859	arg1	Caski					873:877	Caski	873:877	Caski	873:877	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	6	49	from	components	850:859	arg1	A549					864:867	A549	864:867	A549	864:867	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	1	50	theme	outer	139:143	arg1	membrane					154:161	the outer cellular membrane	135:161	the outer cellular membrane	135:161	Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis.					
31717732	6	51	from	GM3	765:767	arg1	NCI-H358					787:794	NCI-H358	787:794	NCI-H358	787:794	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	6	51	from	GM3	765:767	arg1	MCF7					801:804	MCF7	801:804	MCF7	801:804	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	6	51	from	GM3	765:767	arg1	CFPAC1					779:784	CFPAC1	779:784	CFPAC1	779:784	The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively.					
31717732	2	52	theme	biological	234:243	arg1	importance					245:254	the biological importance	230:254	the biological importance of gangliosides	230:270	Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools.					
31717732	3	53	from	elucidation	418:428	arg1	NCI-H358					499:506	NCI-H358	499:506	NCI-H358	499:506	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	53	from	elucidation	418:428	arg1	lines					469:473	various cell lines	456:473	various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski	456:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	53	from	elucidation	418:428	arg1	CFPAC1					485:490	CFPAC1	485:490	CFPAC1	485:490	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	53	from	elucidation	418:428	arg1	MCF7					509:512	MCF7	509:512	MCF7	509:512	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	53	from	elucidation	418:428	arg1	Caski					519:523	Caski	519:523	Caski	519:523	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	3	53	from	elucidation	418:428	arg1	A549					493:496	A549	493:496	A549	493:496	Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski.					
31717732	2	54	theme	tools	355:359	arg1	lack					326:329	the lack	322:329	the lack of effective analytical tools	322:359	Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools.					
31717732	5	55	located	found	632:636	arg1	line					651:654	each cell line	641:654	each cell line	641:654	Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively.					
31717732	5	55	located	found	632:636	arg2	Gangliosides					619:630	Gangliosides	619:630	Gangliosides found in each cell line	619:654	Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively.					
31717732	4	56	theme	cell	585:588	arg1	membrane					590:597	cell membrane	585:597	cell membrane	585:597	We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS.					
31717732	0	57	theme	Cell	71:74	arg1	Lines					76:80	Various Cell Lines	63:80	Various Cell Lines	63:80	Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS.					
31717732	2	58	theme	analytical	344:353	arg1	tools					355:359	effective analytical tools	334:359	effective analytical tools	334:359	Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools.					
31717732	9	59	theme	cell	1336:1339	arg1	types					1341:1345	cell types	1336:1345	cell types	1336:1345	These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.					
30361439	8	0	theme	IgG1	1157:1160	arg1	Fc					1162:1163	IgG1 Fc	1157:1163	IgG1 Fc with about 5.2-fold increased affinity	1157:1202	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	7	1	theme	asparagine-linked	1002:1018	arg1	N-glycans					1035:1043	N-glycans	1035:1043	N-glycans	1035:1043	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	1	theme	asparagine-linked	1002:1018	arg1	carbohydrates					1020:1032	the asparagine-linked carbohydrates	998:1032	the asparagine-linked carbohydrates (N-glycans) attached to the receptor	998:1069	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	1	2	theme	Therapeutic	139:149	arg1	mAbs					151:154	Therapeutic mAbs	139:154	Therapeutic mAbs	139:154	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	1	3	theme	cell-mediated	211:223	arg1	response					225:232	a protective cell-mediated response	198:232	a protective cell-mediated response	198:232	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	6	4	theme	WT	847:848	arg1	CD16a					850:854	WT CD16a	847:854	WT CD16a	847:854	Conversely, the binding affinity of CD16a-G129D was decreased 128-fold relative to WT CD16a and comparably to that of WT CD16b.					
30361439	7	5	theme	carbohydrates	1020:1032	arg1	composition					983:993	the composition	979:993	the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor	979:1069	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	6	attach	attached	1046:1053	arg2	carbohydrates					1020:1032	the asparagine-linked carbohydrates	998:1032	the asparagine-linked carbohydrates (N-glycans) attached to the receptor	998:1069	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	6	attach	attached	1046:1053	arg1	receptor					1062:1069	the receptor	1058:1069	the receptor	1058:1069	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	6	attach	attached	1046:1053	arg2	N-glycans					1035:1043	N-glycans	1035:1043	N-glycans	1035:1043	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	8	7	theme	processed	1239:1247	arg1	N-glycans					1262:1270	highly processed complex-type N-glycans	1232:1270	highly processed complex-type N-glycans	1232:1270	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	6	8	theme	CD16a-G129D	800:810	arg1	affinity					788:795	the binding affinity	776:795	the binding affinity of CD16a-G129D	776:810	Conversely, the binding affinity of CD16a-G129D was decreased 128-fold relative to WT CD16a and comparably to that of WT CD16b.					
30361439	0	9	theme	immunoglobulin	81:94	arg1	G1					96:97	binding immunoglobulin G1	73:97	binding immunoglobulin G1	73:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	10	10	theme	all-atom	1484:1491	arg1	simulation					1512:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	8	11	theme	oligomannose	1125:1136	arg1	N-glycans					1138:1146	minimally processed oligomannose N-glycans	1105:1146	minimally processed oligomannose N-glycans	1105:1146	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	9	12	theme	oligomannose	1359:1370	arg1	N-glycans					1372:1380	oligomannose N-glycans	1359:1380	oligomannose N-glycans	1359:1380	CD16b and the CD16a-G129D variant exhibited a smaller 1.9-fold affinity increase with oligomannose N-glycans.					
30361439	0	13	theme	binding	73:79	arg1	G1					96:97	binding immunoglobulin G1	73:97	binding immunoglobulin G1	73:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	3	14	dep	identity	527:534	arg1	%					525:525	%	525:525	%	525:525	However, it is unclear why IgG binds CD16a with more than 10-fold higher affinity than CD16b even though these receptors share more than 97% identity.					
30361439	9	15	theme	CD16a-G129D	1287:1297	arg1	variant					1299:1305	the CD16a-G129D variant	1283:1305	the CD16a-G129D variant	1283:1305	CD16b and the CD16a-G129D variant exhibited a smaller 1.9-fold affinity increase with oligomannose N-glycans.					
30361439	10	16	theme	Asp-129	1546:1552	arg1	residue					1554:1560	the larger Asp-129 residue	1535:1560	the larger Asp-129 residue	1535:1560	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	10	17	theme	250-ns	1477:1482	arg1	simulation					1512:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	1	18	theme	γ	166:166	arg1	receptor					168:175	Fc γ receptor III	163:179	Fc γ receptor III (CD16) to elicit a protective cell-mediated response	163:232	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	1	18	theme	γ	166:166	arg1	CD16					182:185	CD16	182:185	CD16	182:185	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	4	19	theme	IgG	610:612	arg1	affinity					622:629	the greater IgG binding affinity	598:629	the greater IgG binding affinity of CD16a	598:638	Here we identified one residue, Gly-129, that contributes to the greater IgG binding affinity of CD16a.					
30361439	5	20	theme	higher	735:740	arg1	affinity					742:749	90-fold higher affinity	727:749	90-fold higher affinity than the WT	727:761	The CD16b variant D129G bound IgG1 Fc with 2-fold higher affinity than CD16a and with 90-fold higher affinity than the WT.					
30361439	10	21	theme	Fc-binding	1575:1584	arg1	surface					1586:1592	the Fc-binding surface	1571:1592	the Fc-binding surface	1571:1592	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	10	22	theme	IgG1	1422:1425	arg1	Fc					1427:1428	IgG1 Fc	1422:1428	IgG1 Fc determined to 2.2 Å resolution	1422:1459	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	1	23	theme	Fc	163:164	arg1	receptor					168:175	Fc γ receptor III	163:179	Fc γ receptor III (CD16) to elicit a protective cell-mediated response	163:232	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	1	23	theme	Fc	163:164	arg1	CD16					182:185	CD16	182:185	CD16	182:185	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	0	24	theme	single	2:7	arg1	acid					15:18	A single amino acid	0:18	A single amino acid	0:18	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	11	25	theme	high	1738:1741	arg1	affinity					1743:1750	high affinity	1738:1750	high affinity	1738:1750	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	10	26	theme	CD16b	1407:1411	arg1	model					1385:1389	A model	1383:1389	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation	1383:1521	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	5	27	theme	higher	691:696	arg1	affinity					698:705	2-fold higher affinity	684:705	2-fold higher affinity than CD16a	684:716	The CD16b variant D129G bound IgG1 Fc with 2-fold higher affinity than CD16a and with 90-fold higher affinity than the WT.					
30361439	2	28	theme	CD16b-mediated	360:373	arg1	responses					375:383	protective CD16b-mediated responses	349:383	protective CD16b-mediated responses	349:383	Newer drugs designed to bind CD16a with increased affinity surprisingly also elicit protective CD16b-mediated responses.					
30361439	10	29	theme	larger	1539:1544	arg1	residue					1554:1560	the larger Asp-129 residue	1535:1560	the larger Asp-129 residue	1535:1560	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	10	30	theme	glycosylated	1394:1405	arg1	CD16b					1407:1411	glycosylated CD16b	1394:1411	glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution	1394:1459	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	2	31	theme	protective	349:358	arg1	responses					375:383	protective CD16b-mediated responses	349:383	protective CD16b-mediated responses	349:383	Newer drugs designed to bind CD16a with increased affinity surprisingly also elicit protective CD16b-mediated responses.					
30361439	11	32	from	Asp-129	1620:1626	arg1	CD16b					1631:1635	CD16b	1631:1635	CD16b	1631:1635	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	1	33	theme	target	250:255	arg1	tissue					257:262	the target tissue	246:262	the target tissue	246:262	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
30361439	5	34	theme	CD16b	645:649	arg1	D129G					659:663	The CD16b variant D129G	641:663	The CD16b variant D129G	641:663	The CD16b variant D129G bound IgG1 Fc with 2-fold higher affinity than CD16a and with 90-fold higher affinity than the WT.					
30361439	0	35	theme	amino	9:13	arg1	acid					15:18	A single amino acid	0:18	A single amino acid	0:18	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	8	36	with	variants	1218:1225	arg1	N-glycans					1262:1270	highly processed complex-type N-glycans	1232:1270	highly processed complex-type N-glycans	1232:1270	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	5	37	theme	variant	651:657	arg1	D129G					659:663	The CD16b variant D129G	641:663	The CD16b variant D129G	641:663	The CD16b variant D129G bound IgG1 Fc with 2-fold higher affinity than CD16a and with 90-fold higher affinity than the WT.					
30361439	2	38	with	CD16a	294:298	arg1	affinity					315:322	increased affinity	305:322	increased affinity	305:322	Newer drugs designed to bind CD16a with increased affinity surprisingly also elicit protective CD16b-mediated responses.					
30361439	2	39	theme	Newer	265:269	arg1	drugs					271:275	Newer drugs	265:275	Newer drugs designed to bind CD16a with increased affinity	265:322	Newer drugs designed to bind CD16a with increased affinity surprisingly also elicit protective CD16b-mediated responses.					
30361439	8	40	theme	processed	1115:1123	arg1	N-glycans					1138:1146	minimally processed oligomannose N-glycans	1105:1146	minimally processed oligomannose N-glycans	1105:1146	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	6	41	theme	WT	882:883	arg1	CD16b					885:889	WT CD16b	882:889	WT CD16b	882:889	Conversely, the binding affinity of CD16a-G129D was decreased 128-fold relative to WT CD16a and comparably to that of WT CD16b.					
30361439	8	42	theme	increased	1185:1193	arg1	affinity					1195:1202	about 5.2-fold increased affinity	1170:1202	about 5.2-fold increased affinity	1170:1202	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	11	43	theme	binding	1649:1655	arg1	affinity					1657:1664	its binding affinity	1645:1664	its binding affinity for IgG1 Fc	1645:1676	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	4	44	theme	binding	614:620	arg1	affinity					622:629	the greater IgG binding affinity	598:629	the greater IgG binding affinity of CD16a	598:638	Here we identified one residue, Gly-129, that contributes to the greater IgG binding affinity of CD16a.					
30361439	7	45	link	asparagine-linked	1002:1018	arg1	N-glycans					1035:1043	N-glycans	1035:1043	N-glycans	1035:1043	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	45	link	asparagine-linked	1002:1018	arg1	carbohydrates					1020:1032	the asparagine-linked carbohydrates	998:1032	the asparagine-linked carbohydrates (N-glycans) attached to the receptor	998:1069	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	11	46	theme	Asp-129	1773:1779	arg1	chain					1786:1790	the Asp-129 side chain	1769:1790	the Asp-129 side chain	1769:1790	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	0	47	theme	relative	120:127	arg1	affinity					111:118	affinity	111:118	affinity relative to CD16a	111:136	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	10	48	theme	molecular	1493:1501	arg1	simulation					1512:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	0	49	theme	γ	36:36	arg1	structure					58:66	the Fc γ receptor IIIb/CD16b structure	29:66	the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1	29:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	5	50	theme	IgG1	671:674	arg1	Fc					676:677	IgG1 Fc	671:677	IgG1 Fc	671:677	The CD16b variant D129G bound IgG1 Fc with 2-fold higher affinity than CD16a and with 90-fold higher affinity than the WT.					
30361439	11	51	theme	side	1781:1784	arg1	chain					1786:1790	the Asp-129 side chain	1769:1790	the Asp-129 side chain	1769:1790	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	6	52	theme	binding	780:786	arg1	affinity					788:795	the binding affinity	776:795	the binding affinity of CD16a-G129D	776:810	Conversely, the binding affinity of CD16a-G129D was decreased 128-fold relative to WT CD16a and comparably to that of WT CD16b.					
30361439	2	53	theme	increased	305:313	arg1	affinity					315:322	increased affinity	305:322	increased affinity	305:322	Newer drugs designed to bind CD16a with increased affinity surprisingly also elicit protective CD16b-mediated responses.					
30361439	0	54	theme	Fc	33:34	arg1	structure					58:66	the Fc γ receptor IIIb/CD16b structure	29:66	the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1	29:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	11	55	theme	IgG1	1670:1673	arg1	Fc					1675:1676	IgG1 Fc	1670:1676	IgG1 Fc	1670:1676	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	10	56	theme	Å	1448:1448	arg1	resolution					1450:1459	2.2 Å resolution	1444:1459	2.2 Å resolution	1444:1459	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	11	57	with	CD16b	1727:1731	arg1	affinity					1743:1750	high affinity	1738:1750	high affinity	1738:1750	These results reveal how Asp-129 in CD16b affects its binding affinity for IgG1 Fc and suggest that antibodies engineered to engage CD16b with high affinity must accommodate the Asp-129 side chain.					
30361439	10	58	theme	dynamics	1503:1510	arg1	simulation					1512:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	a 250-ns all-atom molecular dynamics simulation	1475:1521	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	7	59	with	interaction	896:906	arg1	CD16b					944:948	CD16b	944:948	CD16b	944:948	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	59	with	interaction	896:906	arg1	CD16a					924:928	CD16a	924:928	CD16a	924:928	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	60	theme	IgG1	911:914	arg1	Fc					916:917	IgG1 Fc	911:917	IgG1 Fc	911:917	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	3	61	theme	higher	452:457	arg1	affinity					459:466	more than 10-fold higher affinity	434:466	more than 10-fold higher affinity than CD16b	434:477	However, it is unclear why IgG binds CD16a with more than 10-fold higher affinity than CD16b even though these receptors share more than 97% identity.					
30361439	8	62	theme	complex-type	1249:1260	arg1	N-glycans					1262:1270	highly processed complex-type N-glycans	1232:1270	highly processed complex-type N-glycans	1232:1270	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	9	63	theme	smaller	1319:1325	arg1	increase					1345:1352	a smaller 1.9-fold affinity increase	1317:1352	a smaller 1.9-fold affinity increase	1317:1352	CD16b and the CD16a-G129D variant exhibited a smaller 1.9-fold affinity increase with oligomannose N-glycans.					
30361439	0	64	theme	IIIb/CD16b	47:56	arg1	structure					58:66	the Fc γ receptor IIIb/CD16b structure	29:66	the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1	29:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	7	65	theme	Fc	916:917	arg1	interaction					896:906	The interaction	892:906	The interaction of IgG1 Fc with CD16a, but not with CD16b,	892:949	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	7	65	theme	Fc	916:917	arg1	sensitive					966:974	sensitive	966:974	sensitive	966:974	The interaction of IgG1 Fc with CD16a, but not with CD16b, is known to be sensitive to the composition of the asparagine-linked carbohydrates (N-glycans) attached to the receptor.					
30361439	4	66	theme	CD16a	634:638	arg1	affinity					622:629	the greater IgG binding affinity	598:629	the greater IgG binding affinity of CD16a	598:638	Here we identified one residue, Gly-129, that contributes to the greater IgG binding affinity of CD16a.					
30361439	4	67	theme	greater	602:608	arg1	affinity					622:629	the greater IgG binding affinity	598:629	the greater IgG binding affinity of CD16a	598:638	Here we identified one residue, Gly-129, that contributes to the greater IgG binding affinity of CD16a.					
30361439	9	68	theme	1.9-fold	1327:1334	arg1	increase					1345:1352	a smaller 1.9-fold affinity increase	1317:1352	a smaller 1.9-fold affinity increase	1317:1352	CD16b and the CD16a-G129D variant exhibited a smaller 1.9-fold affinity increase with oligomannose N-glycans.					
30361439	0	69	theme	receptor	38:45	arg1	structure					58:66	the Fc γ receptor IIIb/CD16b structure	29:66	the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1	29:97	A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.					
30361439	8	70	with	Fc	1162:1163	arg1	affinity					1195:1202	about 5.2-fold increased affinity	1170:1202	about 5.2-fold increased affinity	1170:1202	CD16a and CD16b-D129G displaying minimally processed oligomannose N-glycans bound to IgG1 Fc with about 5.2-fold increased affinity compared with variants with highly processed complex-type N-glycans.					
30361439	9	71	theme	affinity	1336:1343	arg1	increase					1345:1352	a smaller 1.9-fold affinity increase	1317:1352	a smaller 1.9-fold affinity increase	1317:1352	CD16b and the CD16a-G129D variant exhibited a smaller 1.9-fold affinity increase with oligomannose N-glycans.					
30361439	10	72	gly	glycosylated	1394:1405	arg1	CD16b					1407:1411	glycosylated CD16b	1394:1411	glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution	1394:1459	A model of glycosylated CD16b bound to IgG1 Fc determined to 2.2 Å resolution combined with a 250-ns all-atom molecular dynamics simulation showed that the larger Asp-129 residue deformed the Fc-binding surface.					
30361439	3	73	dep	%	525:525	arg1	97					523:524	97	523:524	97	523:524	However, it is unclear why IgG binds CD16a with more than 10-fold higher affinity than CD16b even though these receptors share more than 97% identity.					
30361439	1	74	theme	protective	200:209	arg1	response					225:232	a protective cell-mediated response	198:232	a protective cell-mediated response	198:232	Therapeutic mAbs engage Fc γ receptor III (CD16) to elicit a protective cell-mediated response and destroy the target tissue.					
31631317	1	0	theme	biopharmaceutical	136:152	arg1	industry					154:161	the biopharmaceutical industry	132:161	the biopharmaceutical industry	132:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	1	theme	CHO	246:248	arg1	cells					250:254	CHO cells	246:254	CHO cells	246:254	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	3	2	theme	appropriate	531:541	arg1	structures					550:559	the appropriate glycan structures	527:559	the appropriate glycan structures	527:559	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	1	3	theme	Chinese	66:72	arg1	workhorse					118:126	the preferred workhorse	104:126	the preferred workhorse for the biopharmaceutical industry	104:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	3	theme	Chinese	66:72	arg1	cells					94:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	3	4	theme	glycan	543:548	arg1	structures					550:559	the appropriate glycan structures	527:559	the appropriate glycan structures	527:559	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	2	5	with	toolbox	296:302	arg1	CRISPRa					328:334	CRISPRa	328:334	CRISPRa	328:334	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	2	5	with	toolbox	296:302	arg1	activation					316:325	CRISPR activation	309:325	CRISPR activation (CRISPRa)	309:335	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	1	6	theme	hamster	74:80	arg1	workhorse					118:126	the preferred workhorse	104:126	the preferred workhorse for the biopharmaceutical industry	104:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	6	theme	hamster	74:80	arg1	cells					94:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	4	7	theme	desired	660:666	arg1	phenotypes					668:677	desired phenotypes	660:677	desired phenotypes	660:677	This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.					
31631317	3	8	from	activity	465:472	arg1	level					490:494	a functional level	477:494	a functional level	477:494	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	1	9	theme	ovary	82:86	arg1	workhorse					118:126	the preferred workhorse	104:126	the preferred workhorse for the biopharmaceutical industry	104:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	9	theme	ovary	82:86	arg1	cells					94:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	3	10	theme	St6gal1	437:443	arg1	transcription					410:422	transcription	410:422	transcription of Mgat3 and St6gal1	410:443	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	0	11	theme	dormant	10:16	arg1	glycosyltransferases					18:37	dormant glycosyltransferases	10:37	dormant glycosyltransferases	10:37	Awakening dormant glycosyltransferases in CHO cells with CRISPRa.					
31631317	2	12	theme	endogenous	366:375	arg1	genes					377:381	endogenous genes	366:381	endogenous genes	366:381	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	1	13	theme	CHO	89:91	arg1	workhorse					118:126	the preferred workhorse	104:126	the preferred workhorse for the biopharmaceutical industry	104:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	13	theme	CHO	89:91	arg1	cells					94:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	3	14	theme	Mgat3	427:431	arg1	transcription					410:422	transcription	410:422	transcription of Mgat3 and St6gal1	410:443	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	2	15	theme	engineering	284:294	arg1	toolbox					296:302	the CRISPR engineering toolbox	273:302	the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes	273:381	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	2	16	theme	CRISPR	277:282	arg1	toolbox					296:302	the CRISPR engineering toolbox	273:302	the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes	273:381	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	4	17	theme	cells	650:654	arg1	alterations					631:641	targeted alterations	622:641	targeted alterations of CHO cells for desired phenotypes	622:677	This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.					
31631317	0	18	theme	CHO	42:44	arg1	cells					46:50	CHO cells	42:50	CHO cells with CRISPRa	42:63	Awakening dormant glycosyltransferases in CHO cells with CRISPRa.					
31631317	4	19	theme	CHO	646:648	arg1	cells					650:654	CHO cells	646:654	CHO cells	646:654	This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.					
31631317	3	20	theme	functional	479:488	arg1	level					490:494	a functional level	477:494	a functional level	477:494	We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced.					
31631317	1	21	theme	preferred	108:116	arg1	workhorse					118:126	the preferred workhorse	104:126	the preferred workhorse for the biopharmaceutical industry	104:161	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	21	theme	preferred	108:116	arg1	cells					94:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells	66:98	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	1	22	theme	targeted	215:222	arg1	perturbations					229:241	targeted gene perturbations	215:241	targeted gene perturbations	215:241	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	2	23	theme	CRISPR	309:314	arg1	CRISPRa					328:334	CRISPRa	328:334	CRISPRa	328:334	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	2	23	theme	CRISPR	309:314	arg1	activation					316:325	CRISPR activation	309:325	CRISPR activation (CRISPRa)	309:335	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	1	24	theme	gene	224:227	arg1	perturbations					229:241	targeted gene perturbations	215:241	targeted gene perturbations	215:241	Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells.					
31631317	2	25	theme	genes	377:381	arg1	transcription					349:361	transcription	349:361	transcription of endogenous genes	349:381	Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.					
31631317	4	26	theme	targeted	622:629	arg1	alterations					631:641	targeted alterations	622:641	targeted alterations of CHO cells for desired phenotypes	622:677	This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.					
31631317	0	27	with	cells	46:50	arg1	CRISPRa					57:63	CRISPRa	57:63	CRISPRa	57:63	Awakening dormant glycosyltransferases in CHO cells with CRISPRa.					
29533603	0	0	theme	Stable	93:98	arg1	Primer					127:132	a Stable Isotope-Labeled Saccharide Primer	91:132	a Stable Isotope-Labeled Saccharide Primer	91:132	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	3	1	gly	glycosylated	838:849	arg1	products					851:858	glycosylated products	838:858	glycosylated products	838:858	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	4	2	theme	deuterium-labeled	925:941	arg1	primer					954:959	a deuterium-labeled saccharide primer	923:959	a deuterium-labeled saccharide primer	923:959	In this study, we developed a new quantification method using a deuterium-labeled saccharide primer.					
29533603	6	3	gly	glycosylated	1234:1245	arg1	products					1247:1254	glycosylated products	1234:1254	glycosylated products	1234:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	3	4	theme	solid-phase	723:733	arg1	extraction					735:744	solid-phase extraction	723:744	solid-phase extraction in analysis	723:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	5	dep	structure	600:608	arg1	the					596:598	the	596:598	the	596:598	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	0	6	theme	Saccharide	116:125	arg1	Primer					127:132	a Stable Isotope-Labeled Saccharide Primer	91:132	a Stable Isotope-Labeled Saccharide Primer	91:132	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	6	7	theme	products	1247:1254	arg1	amounts					1223:1229	the theoretical amounts	1207:1229	the theoretical amounts of glycosylated products	1207:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	6	7	theme	products	1247:1254	arg1	products					1247:1254	glycosylated products	1234:1254	glycosylated products	1234:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	6	8	theme	theoretical	1211:1221	arg1	amounts					1223:1229	the theoretical amounts	1207:1229	the theoretical amounts of glycosylated products	1207:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	6	8	theme	theoretical	1211:1221	arg1	products					1247:1254	glycosylated products	1234:1254	glycosylated products	1234:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	0	9	theme	Isotope-Labeled	100:114	arg1	Primer					127:132	a Stable Isotope-Labeled Saccharide Primer	91:132	a Stable Isotope-Labeled Saccharide Primer	91:132	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	3	10	theme	experimental	768:779	arg1	error					781:785	experimental error	768:785	experimental error	768:785	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	4	11	theme	saccharide	943:952	arg1	primer					954:959	a deuterium-labeled saccharide primer	923:959	a deuterium-labeled saccharide primer	923:959	In this study, we developed a new quantification method using a deuterium-labeled saccharide primer.					
29533603	7	12	theme	biosynthesis	1303:1314	arg1	assay					1327:1331	a biosynthesis inhibition assay	1301:1331	a biosynthesis inhibition assay using known GAG biosynthesis inhibitors	1301:1371	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	8	13	theme	saccharide	1421:1430	arg1	primers					1432:1438	saccharide primers	1421:1438	saccharide primers	1421:1438	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	1	14	theme	cellular	284:291	arg1	differentiation					323:337	differentiation	323:337	differentiation	323:337	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	1	14	theme	cellular	284:291	arg1	processes					293:301	cellular processes	284:301	cellular processes such as disease and differentiation	284:337	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	1	14	theme	cellular	284:291	arg1	disease					311:317	disease	311:317	disease	311:317	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	5	15	theme	"	972:972	arg1	primers					986:992	The "heavy" and "light" primers	962:992	The "heavy" and "light" primers	962:992	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	4	16	theme	quantification	895:908	arg1	method					910:915	a new quantification method	889:915	a new quantification method using a deuterium-labeled saccharide primer	889:959	In this study, we developed a new quantification method using a deuterium-labeled saccharide primer.					
29533603	9	17	theme	comparative	1636:1646	arg1	glycosaminoglycomics					1648:1667	comparative glycosaminoglycomics	1636:1667	comparative glycosaminoglycomics using cultured cells and tissues	1636:1700	Our results indicate that the method will likely forge a new path for comparative glycosaminoglycomics using cultured cells and tissues.					
29533603	8	18	theme	relative	1391:1398	arg1	quantification					1400:1413	the relative quantification	1387:1413	the relative quantification using saccharide primers	1387:1438	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	4	19	theme	new	891:893	arg1	method					910:915	a new quantification method	889:915	a new quantification method using a deuterium-labeled saccharide primer	889:959	In this study, we developed a new quantification method using a deuterium-labeled saccharide primer.					
29533603	1	20	dep	structures	139:148	arg1	The					135:137	The	135:137	The	135:137	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	6	21	theme	good	1162:1165	arg1	linearity					1167:1175	good linearity	1162:1175	good linearity	1162:1175	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	2	22	theme	artificial	403:412	arg1	acceptors					414:422	artificial acceptors	403:422	artificial acceptors	403:422	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	2	22	theme	artificial	403:412	arg1	β-Xylosides					340:350	β-Xylosides	340:350	β-Xylosides	340:350	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	0	23	theme	Quantification	12:25	arg1	Method					27:32	Comparative Quantification Method	0:32	Comparative Quantification Method for Glycosylated Products	0:58	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	6	24	theme	glycosylated	1234:1245	arg1	products					1247:1254	glycosylated products	1234:1254	glycosylated products	1234:1254	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	7	25	theme	inhibition	1316:1325	arg1	assay					1327:1331	a biosynthesis inhibition assay	1301:1331	a biosynthesis inhibition assay using known GAG biosynthesis inhibitors	1301:1371	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	5	26	theme	"	984:984	arg1	primers					986:992	The "heavy" and "light" primers	962:992	The "heavy" and "light" primers	962:992	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	7	27	theme	known	1339:1343	arg1	inhibitors					1362:1371	known GAG biosynthesis inhibitors	1339:1371	known GAG biosynthesis inhibitors	1339:1371	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	6	28	theme	light/heavy	1134:1144	arg1	products					1146:1153	light/heavy products	1134:1153	light/heavy products	1134:1153	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	0	29	theme	Comparative	0:10	arg1	Method					27:32	Comparative Quantification Method	0:32	Comparative Quantification Method for Glycosylated Products	0:58	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	3	30	theme	accurate	799:806	arg1	quantification					820:833	accurate comparative quantification	799:833	accurate comparative quantification of glycosylated products	799:858	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	9	31	theme	cultured	1675:1682	arg1	cells					1684:1688	cells	1684:1688	cells	1684:1688	Our results indicate that the method will likely forge a new path for comparative glycosaminoglycomics using cultured cells and tissues.					
29533603	3	32	theme	comparative	808:818	arg1	quantification					820:833	accurate comparative quantification	799:833	accurate comparative quantification of glycosylated products	799:858	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	5	33	theme	light	979:983	arg1	"					984:984	"light"	978:984	"light"	978:984	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	6	34	theme	Relative	1111:1118	arg1	areas					1125:1129	Relative peak areas	1111:1129	Relative peak areas of light/heavy products	1111:1153	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	2	35	theme	GAG	445:447	arg1	oligosaccharides					449:464	GAG oligosaccharides	445:464	GAG oligosaccharides in cells and tissues	445:485	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	8	36	theme	GAG	1540:1542	arg1	pathway					1557:1563	the GAG biosynthetic pathway	1536:1563	the GAG biosynthetic pathway	1536:1563	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	5	37	theme	heavy	967:971	arg1	"					972:972	"heavy"	966:972	"heavy"	966:972	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	9	38	theme	new	1623:1625	arg1	path					1627:1630	a new path	1621:1630	a new path for comparative glycosaminoglycomics using cultured cells and tissues	1621:1700	Our results indicate that the method will likely forge a new path for comparative glycosaminoglycomics using cultured cells and tissues.					
29533603	6	39	theme	peak	1120:1123	arg1	areas					1125:1129	Relative peak areas	1111:1129	Relative peak areas of light/heavy products	1111:1153	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	7	40	theme	validation	1268:1277	arg1	study					1279:1283	a validation study	1266:1283	a validation study	1266:1283	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	3	41	theme	sample	686:691	arg1	processes					705:713	sample preparation processes	686:713	sample preparation processes such as solid-phase extraction in analysis	686:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	41	theme	sample	686:691	arg1	extraction					735:744	solid-phase extraction	723:744	solid-phase extraction in analysis	723:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	5	42	theme	liquid	1063:1068	arg1	spectrometry					1097:1108	liquid chromatography-tandem mass spectrometry	1063:1108	liquid chromatography-tandem mass spectrometry	1063:1108	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	1	43	theme	much	222:225	arg1	interest					227:234	much interest	222:234	much interest	222:234	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	0	44	theme	Glycosylated	38:49	arg1	Products					51:58	Glycosylated Products	38:58	Glycosylated Products	38:58	Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer.					
29533603	3	45	theme	glycosylated	838:849	arg1	products					851:858	glycosylated products	838:858	glycosylated products	838:858	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	46	from	extraction	735:744	arg1	analysis					749:756	analysis	749:756	analysis	749:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	47	theme	GAG	625:627	arg1	oligosaccharides					629:644	GAG oligosaccharides	625:644	GAG oligosaccharides elongated using saccharide primers	625:679	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	48	theme	products	851:858	arg1	quantification					820:833	accurate comparative quantification	799:833	accurate comparative quantification of glycosylated products	799:858	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	8	49	theme	biosynthetic	1544:1555	arg1	pathway					1557:1563	the GAG biosynthetic pathway	1536:1563	the GAG biosynthetic pathway	1536:1563	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	5	50	theme	mass	1092:1095	arg1	spectrometry					1097:1108	liquid chromatography-tandem mass spectrometry	1063:1108	liquid chromatography-tandem mass spectrometry	1063:1108	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	2	51	theme	saccharide	365:374	arg1	primers					376:382	saccharide primers	365:382	saccharide primers	365:382	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	8	52	theme	biosynthesis	1494:1505	arg1	inhibitors					1507:1516	the four GAG biosynthesis inhibitors	1481:1516	the four GAG biosynthesis inhibitors	1481:1516	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	8	53	theme	GAG	1490:1492	arg1	inhibitors					1507:1516	the four GAG biosynthesis inhibitors	1481:1516	the four GAG biosynthesis inhibitors	1481:1516	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	7	54	theme	GAG	1345:1347	arg1	inhibitors					1362:1371	known GAG biosynthesis inhibitors	1339:1371	known GAG biosynthesis inhibitors	1339:1371	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	7	55	theme	biosynthesis	1349:1360	arg1	inhibitors					1362:1371	known GAG biosynthesis inhibitors	1339:1371	known GAG biosynthesis inhibitors	1339:1371	Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors.					
29533603	8	56	from	differences	1441:1451	arg1	mode-of-action					1460:1473	the mode-of-action	1456:1473	the mode-of-action	1456:1473	According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway.					
29533603	5	57	theme	chromatography-tandem	1070:1090	arg1	spectrometry					1097:1108	liquid chromatography-tandem mass spectrometry	1063:1108	liquid chromatography-tandem mass spectrometry	1063:1108	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	5	58	theme	priming	1027:1033	arg1	abilities					1035:1043	priming abilities	1027:1043	priming abilities	1027:1043	The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry.					
29533603	3	59	theme	preparation	693:703	arg1	processes					705:713	sample preparation processes	686:713	sample preparation processes such as solid-phase extraction in analysis	686:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	59	theme	preparation	693:703	arg1	extraction					735:744	solid-phase extraction	723:744	solid-phase extraction in analysis	723:756	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	60	theme	saccharide	662:671	arg1	primers					673:679	saccharide primers	662:679	saccharide primers	662:679	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	2	61	used	used	395:398	arg2	β-Xylosides					340:350	β-Xylosides	340:350	β-Xylosides	340:350	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	2	61	used	used	395:398	arg2	acceptors					414:422	artificial acceptors	403:422	artificial acceptors	403:422	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	3	62	theme	analytical	548:557	arg1	methods					559:565	Various analytical methods	540:565	Various analytical methods	540:565	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	1	63	theme	GAG	244:246	arg1	activity					261:268	GAG biosynthesis activity	244:268	GAG biosynthesis activity	244:268	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	2	64	theme	biosynthetic	517:528	arg1	pathways					530:537	their biosynthetic pathways	511:537	their biosynthetic pathways	511:537	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	3	65	theme	oligosaccharides	629:644	arg1	amounts					614:620	amounts	614:620	amounts	614:620	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	65	theme	oligosaccharides	629:644	arg1	oligosaccharides					629:644	GAG oligosaccharides	625:644	GAG oligosaccharides elongated using saccharide primers	625:679	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	65	theme	oligosaccharides	629:644	arg1	structure					600:608	structure	600:608	structure	600:608	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	3	66	theme	Various	540:546	arg1	methods					559:565	Various analytical methods	540:565	Various analytical methods	540:565	Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products.					
29533603	6	67	theme	products	1146:1153	arg1	areas					1125:1129	Relative peak areas	1111:1129	Relative peak areas of light/heavy products	1111:1153	Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products.					
29533603	1	68	theme	biosynthesis	248:259	arg1	activity					261:268	GAG biosynthesis activity	244:268	GAG biosynthesis activity	244:268	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	2	69	from	oligosaccharides	449:464	arg1	tissues					479:485	tissues	479:485	tissues	479:485	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	2	69	from	oligosaccharides	449:464	arg1	cells					469:473	cells	469:473	cells	469:473	β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways.					
29533603	1	70	theme	glycosaminoglycan	165:181	arg1	glycosaminoglycan					165:181	glycosaminoglycan	165:181	glycosaminoglycan (GAG) produced by cells	165:205	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	1	70	theme	glycosaminoglycan	165:181	arg1	amounts					154:160	amounts	154:160	amounts	154:160	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	1	70	theme	glycosaminoglycan	165:181	arg1	structures					139:148	structures	139:148	structures	139:148	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
29533603	1	70	theme	glycosaminoglycan	165:181	arg1	GAG					184:186	GAG	184:186	GAG	184:186	The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation.					
31086181	4	0	theme	glycans	748:754	arg1	analysis					736:743	the analysis	732:743	the analysis of glycans at the attomole level	732:776	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	1	1	dep	in-depth	86:93	arg1	high-sensitivity					96:111	high-sensitivity	96:111	high-sensitivity	96:111	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	2	theme	biological	242:251	arg1	research					253:260	basic biological research	236:260	basic biological research	236:260	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	4	3	from	amounts	915:921	arg1	available					895:903	available	895:903	available	895:903	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	4	4	theme	minute	908:913	arg1	amounts					915:921	minute amounts	908:921	minute amounts	908:921	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	1	5	from	samples	169:175	arg1	glycome					137:143	the glycome	133:143	the glycome from complex biological samples, such as biofluids and tissues,	133:207	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	5	from	samples	169:175	arg1	characterization					113:128	The in-depth, high-sensitivity characterization	82:128	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues,	82:207	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	2	6	theme	glycans	354:360	arg1	diversity					341:349	the vast structural diversity	321:349	the vast structural diversity of glycans in combination with limited sample amounts	321:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	4	7	from	analysis	736:743	arg1	level					772:776	the attomole level	759:776	the attomole level	759:776	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	4	8	theme	acid	810:813	arg1	isomers					815:821	sialic acid isomers	803:821	sialic acid isomers	803:821	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	3	9	theme	linkage-specific	605:620	arg1	derivatization					622:635	linkage-specific derivatization	605:635	linkage-specific derivatization of sialic acids	605:651	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	4	10	theme	sialic	803:808	arg1	isomers					815:821	sialic acid isomers	803:821	sialic acid isomers	803:821	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	2	11	theme	Major	287:291	arg1	challenges					293:302	Major challenges	287:302	Major challenges	287:302	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	1	12	theme	biomarker	266:274	arg1	discovery					276:284	biomarker discovery	266:284	biomarker discovery	266:284	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	4	13	from	available	895:903	arg1	amounts					915:921	minute amounts	908:921	minute amounts	908:921	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	2	14	from	diversity	341:349	arg1	combination					365:375	combination	365:375	combination with limited sample amounts	365:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	3	15	theme	spectrometry	566:577	arg1	approach					591:598	a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach	509:598	a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach	509:598	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	0	16	theme	sensitive	7:15	arg1	analysis					27:34	Highly sensitive CE-ESI-MS analysis	0:34	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.	0:80	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	17	theme	ionization-mass	550:564	arg1	CE-ESI-MS					580:588	CE-ESI-MS	580:588	CE-ESI-MS	580:588	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	17	theme	ionization-mass	550:564	arg1	spectrometry					566:577	capillary electrophoresis-electrospray ionization-mass spectrometry	511:577	a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach	509:598	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	4	18	from	information	788:798	arg1	isomers					815:821	sialic acid isomers	803:821	sialic acid isomers	803:821	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	1	19	theme	in-depth	86:93	arg1	characterization					113:128	The in-depth, high-sensitivity characterization	82:128	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues,	82:207	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	2	20	theme	sample	390:395	arg1	amounts					397:403	limited sample amounts	382:403	limited sample amounts	382:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	4	21	theme	attomole	763:770	arg1	level					772:776	the attomole level	759:776	the attomole level	759:776	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	2	22	theme	limited	382:388	arg1	amounts					397:403	limited sample amounts	382:403	limited sample amounts	382:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	4	23	theme	samples	876:882	arg1	characterization					848:863	the in-depth characterization	835:863	the in-depth characterization of complex samples	835:882	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	4	24	theme	complex	868:874	arg1	samples					876:882	complex samples	868:882	complex samples	868:882	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	0	25	theme	CE-ESI-MS	17:25	arg1	analysis					27:34	Highly sensitive CE-ESI-MS analysis	0:34	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.	0:80	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	26	theme	capillary	511:519	arg1	CE-ESI-MS					580:588	CE-ESI-MS	580:588	CE-ESI-MS	580:588	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	26	theme	capillary	511:519	arg1	spectrometry					566:577	capillary electrophoresis-electrospray ionization-mass spectrometry	511:577	a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach	509:598	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	27	theme	electrophoresis-electrospray	521:548	arg1	CE-ESI-MS					580:588	CE-ESI-MS	580:588	CE-ESI-MS	580:588	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	27	theme	electrophoresis-electrospray	521:548	arg1	spectrometry					566:577	capillary electrophoresis-electrospray ionization-mass spectrometry	511:577	a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach	509:598	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	0	28	theme	N-glycans	39:47	arg1	analysis					27:34	Highly sensitive CE-ESI-MS analysis	0:34	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.	0:80	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	29	theme	glycans	704:710	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	29	theme	glycans	704:710	arg1	derivatization					622:635	linkage-specific derivatization	605:635	linkage-specific derivatization of sialic acids	605:651	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	30	theme	acids	647:651	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	30	theme	acids	647:651	arg1	derivatization					622:635	linkage-specific derivatization	605:635	linkage-specific derivatization of sialic acids	605:651	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	0	31	from	samples	73:79	arg1	analysis					27:34	Highly sensitive CE-ESI-MS analysis	0:34	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.	0:80	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	32	theme	sensitive	447:455	arg1	characterization					457:472	the highly sensitive characterization	436:472	the highly sensitive characterization of released N-glycans	436:494	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	0	33	theme	complex	54:60	arg1	samples					73:79	complex biological samples	54:79	complex biological samples	54:79	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	34	theme	released	477:484	arg1	N-glycans					486:494	released N-glycans	477:494	released N-glycans	477:494	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	3	35	theme	sialic	640:645	arg1	acids					647:651	sialic acids	640:651	sialic acids	640:651	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	4	36	from	level	772:776	arg1	glycans					748:754	glycans	748:754	glycans at the attomole level	748:776	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	4	36	from	level	772:776	arg1	analysis					736:743	the analysis	732:743	the analysis of glycans at the attomole level	732:776	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	4	37	theme	in-depth	839:846	arg1	characterization					848:863	the in-depth characterization	835:863	the in-depth characterization of complex samples	835:882	This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.					
31086181	3	38	theme	reducing	674:681	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	1	39	theme	glycome	137:143	arg1	characterization					113:128	The in-depth, high-sensitivity characterization	82:128	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues,	82:207	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	40	from	characterization	113:128	arg1	biofluids					186:194	biofluids	186:194	biofluids	186:194	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	40	from	characterization	113:128	arg1	samples					169:175	complex biological samples	150:175	complex biological samples	150:175	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	40	from	characterization	113:128	arg1	tissues					200:206	tissues	200:206	tissues	200:206	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	41	theme	utmost	215:220	arg1	importance					222:231	utmost importance	215:231	utmost importance	215:231	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	42	from	importance	222:231	arg1	research					253:260	basic biological research	236:260	basic biological research	236:260	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	42	from	importance	222:231	arg1	discovery					276:284	biomarker discovery	266:284	biomarker discovery	266:284	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	3	43	theme	end	683:685	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	2	44	theme	vast	325:328	arg1	diversity					341:349	the vast structural diversity	321:349	the vast structural diversity of glycans in combination with limited sample amounts	321:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	2	45	theme	structural	330:339	arg1	diversity					341:349	the vast structural diversity	321:349	the vast structural diversity of glycans in combination with limited sample amounts	321:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	2	46	with	combination	365:375	arg1	amounts					397:403	limited sample amounts	382:403	limited sample amounts	382:403	Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts.					
31086181	3	47	theme	uniform	657:663	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	1	48	theme	complex	150:156	arg1	biofluids					186:194	biofluids	186:194	biofluids	186:194	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	48	theme	complex	150:156	arg1	samples					169:175	complex biological samples	150:175	complex biological samples	150:175	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	48	theme	complex	150:156	arg1	tissues					200:206	tissues	200:206	tissues	200:206	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	3	49	theme	N-glycans	486:494	arg1	characterization					457:472	the highly sensitive characterization	436:472	the highly sensitive characterization of released N-glycans	436:494	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	0	50	theme	biological	62:71	arg1	samples					73:79	complex biological samples	54:79	complex biological samples	54:79	Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.					
31086181	3	51	theme	cationic	665:672	arg1	labelling					687:695	uniform cationic reducing end labelling	657:695	uniform cationic reducing end labelling of all glycans	657:710	Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans.					
31086181	1	52	theme	biological	158:167	arg1	biofluids					186:194	biofluids	186:194	biofluids	186:194	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	52	theme	biological	158:167	arg1	samples					169:175	complex biological samples	150:175	complex biological samples	150:175	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	52	theme	biological	158:167	arg1	tissues					200:206	tissues	200:206	tissues	200:206	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
31086181	1	53	theme	basic	236:240	arg1	research					253:260	basic biological research	236:260	basic biological research	236:260	The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery.					
30535529	1	0	theme	powerful	312:319	arg1	techniques					321:330	both powerful techniques	307:330	both powerful techniques for the separation of complex samples	307:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	0	theme	powerful	312:319	arg1	electrophoresis					154:168	Capillary zone electrophoresis	139:168	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase	139:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	0	theme	powerful	312:319	arg1	spectrometry					247:258	ion mobility spectrometry	234:258	ion mobility spectrometry (IMS) based on mobilities in the gas phase	234:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	4	1	with	coupling	660:667	arg1	DTIM-MS					724:730	DTIM-MS	724:730	DTIM-MS	724:730	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	1	with	coupling	660:667	arg1	spectrometry					710:721	drift tube ion mobility-mass spectrometry	681:721	drift tube ion mobility-mass spectrometry (DTIM-MS)	681:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	6	2	theme	glycan	1071:1076	arg1	signal					1078:1083	Each individual glycan signal	1055:1083	Each individual glycan signal separated in CZE	1055:1100	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	4	3	theme	CZE	672:674	arg1	coupling					660:667	the first on-line coupling	642:667	the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS)	642:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	5	4	theme	APTS-labeled	1033:1044	arg1	glycans					1046:1052	both native and APTS-labeled glycans	1017:1052	both native and APTS-labeled glycans	1017:1052	In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans.					
30535529	8	5	attach	attached	1359:1366	arg2	acid					1354:1357	sialic acid	1347:1357	sialic acid attached to glycans	1347:1377	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	8	5	attach	attached	1359:1366	arg1	glycans					1371:1377	glycans	1371:1377	glycans	1371:1377	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	4	6	theme	-labeled	868:875	arg1	N-glycans					877:885	native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	814:885	native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	814:885	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	6	7	theme	peaks	1143:1147	arg1	number					1133:1138	an unexpectedly high number	1112:1138	an unexpectedly high number of peaks observed in the IMS dimension	1112:1177	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	8	8	theme	sialic	1347:1352	arg1	acid					1354:1357	sialic acid	1347:1357	sialic acid attached to glycans	1347:1377	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	2	9	theme	diseases	515:522	arg1	range					475:479	a wide range	468:479	a wide range of biological functions and human diseases	468:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	9	10	theme	α2-3	1472:1475	arg1	neuraminidase					1477:1489	α2-3 neuraminidase	1472:1489	α2-3 neuraminidase	1472:1489	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	10	11	theme	various	1705:1711	arg1	applications					1713:1724	various applications	1705:1724	various applications	1705:1724	This work is a showcase for the high potential of CZE-DTIM-MS, which is expected to find various applications in the future.					
30535529	3	12	theme	high	538:541	arg1	variability					554:564	their high structural variability	532:564	their high structural variability	532:564	Due to their high structural variability, the analysis of glycans still represents a challenging task.					
30535529	10	13	theme	CZE-DTIM-MS	1666:1676	arg1	potential					1653:1661	the high potential	1644:1661	the high potential of CZE-DTIM-MS	1644:1676	This work is a showcase for the high potential of CZE-DTIM-MS, which is expected to find various applications in the future.					
30535529	4	14	theme	ion	692:694	arg1	DTIM-MS					724:730	DTIM-MS	724:730	DTIM-MS	724:730	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	14	theme	ion	692:694	arg1	spectrometry					710:721	drift tube ion mobility-mass spectrometry	681:721	drift tube ion mobility-mass spectrometry (DTIM-MS)	681:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	2	15	theme	human	509:513	arg1	diseases					515:522	human diseases	509:522	human diseases	509:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	7	16	theme	forms	1256:1260	arg1	presence					1235:1242	the presence	1231:1242	the presence of isomeric forms, including different linkages, and/or gas-phase conformers	1231:1319	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	1	17	from	mobilities	275:284	arg1	phase					297:301	the gas phase	289:301	the gas phase	289:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	9	18	theme	peaks	1525:1529	arg1	assignment					1511:1520	the partial assignment	1499:1520	the partial assignment of peaks	1499:1529	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	0	19	theme	native	104:109	arg1	N-glycans					128:136	native and APTS-labeled N-glycans	104:136	native and APTS-labeled N-glycans	104:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	2	20	theme	wide	470:473	arg1	range					475:479	a wide range	468:479	a wide range of biological functions and human diseases	468:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	6	21	theme	high	1128:1131	arg1	number					1133:1138	an unexpectedly high number	1112:1138	an unexpectedly high number of peaks observed in the IMS dimension	1112:1177	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	2	22	theme	post-translational	419:436	arg1	modifications					438:450	the most common post-translational modifications	403:450	the most common post-translational modifications associated with a wide range of biological functions and human diseases	403:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	2	23	theme	Protein	371:377	arg1	glycosylation					379:391	Protein glycosylation	371:391	Protein glycosylation	371:391	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	0	24	theme	zone	10:13	arg1	electrophoresis					15:29	Capillary zone electrophoresis	0:29	Capillary zone electrophoresis	0:29	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	1	25	theme	complex	354:360	arg1	samples					362:368	complex samples	354:368	complex samples	354:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	7	26	theme	isomeric	1247:1254	arg1	linkages					1283:1290	different linkages	1273:1290	different linkages	1273:1290	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	7	26	theme	isomeric	1247:1254	arg1	conformers					1310:1319	gas-phase conformers	1300:1319	gas-phase conformers	1300:1319	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	7	26	theme	isomeric	1247:1254	arg1	forms					1256:1260	isomeric forms	1247:1260	isomeric forms	1247:1260	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	4	27	theme	on-line	652:658	arg1	coupling					660:667	the first on-line coupling	642:667	the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS)	642:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	0	28	theme	Capillary	0:8	arg1	electrophoresis					15:29	Capillary zone electrophoresis	0:29	Capillary zone electrophoresis	0:29	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	4	29	theme	8-aminopyrene-1,3,6-trisulfonic	825:855	arg1	APTS					863:866	APTS	863:866	APTS	863:866	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	29	theme	8-aminopyrene-1,3,6-trisulfonic	825:855	arg1	acid					857:860	8-aminopyrene-1,3,6-trisulfonic acid	825:860	8-aminopyrene-1,3,6-trisulfonic acid (APTS)	825:867	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	10	30	theme	high	1648:1651	arg1	potential					1653:1661	the high potential	1644:1661	the high potential of CZE-DTIM-MS	1644:1676	This work is a showcase for the high potential of CZE-DTIM-MS, which is expected to find various applications in the future.					
30535529	5	31	theme	glycan	917:922	arg1	signals					924:930	glycan signals	917:930	glycan signals	917:930	In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans.					
30535529	1	32	theme	electrophoretic	185:199	arg1	mobility					201:208	electrophoretic mobility	185:208	electrophoretic mobility in the liquid phase	185:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	2	33	theme	common	412:417	arg1	modifications					438:450	the most common post-translational modifications	403:450	the most common post-translational modifications associated with a wide range of biological functions and human diseases	403:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	0	34	theme	N-glycans	128:136	arg1	analysis					92:99	the analysis	88:99	the analysis of native and APTS-labeled N-glycans	88:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	7	35	theme	gas-phase	1300:1308	arg1	conformers					1310:1319	gas-phase conformers	1300:1319	gas-phase conformers	1300:1319	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	3	36	theme	structural	543:552	arg1	variability					554:564	their high structural variability	532:564	their high structural variability	532:564	Due to their high structural variability, the analysis of glycans still represents a challenging task.					
30535529	8	37	from	impact	1397:1402	arg1	profile					1431:1437	the obtained drift time profile	1407:1437	the obtained drift time profile	1407:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	9	38	theme	arrival	1538:1544	arg1	distribution					1551:1562	the arrival time distribution	1534:1562	the arrival time distribution considering their sialic acid linkages (α2-3/α2-6)	1534:1613	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	11	39	theme	Graphical	1741:1749	arg1	abstract					1751:1758	Graphical abstract	1741:1758	Graphical abstract	1741:1758	Graphical abstract ᅟ.					
30535529	0	40	theme	APTS-labeled	115:126	arg1	N-glycans					128:136	native and APTS-labeled N-glycans	104:136	native and APTS-labeled N-glycans	104:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	1	41	theme	samples	362:368	arg1	separation					340:349	the separation	336:349	the separation of complex samples	336:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	6	42	theme	individual	1060:1069	arg1	signal					1078:1083	Each individual glycan signal	1055:1083	Each individual glycan signal separated in CZE	1055:1100	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	9	43	theme	partial	1503:1509	arg1	assignment					1511:1520	the partial assignment	1499:1520	the partial assignment of peaks	1499:1529	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	9	44	theme	neuraminidase	1477:1489	arg1	application					1457:1467	the application	1453:1467	the application of α2-3 neuraminidase	1453:1489	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	0	45	theme	drift	42:46	arg1	spectrometry					71:82	drift tube ion mobility-mass spectrometry	42:82	drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans	42:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	7	46	theme	different	1273:1281	arg1	linkages					1283:1290	different linkages	1273:1290	different linkages	1273:1290	This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers.					
30535529	9	47	theme	time	1546:1549	arg1	distribution					1551:1562	the arrival time distribution	1534:1562	the arrival time distribution considering their sialic acid linkages (α2-3/α2-6)	1534:1613	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	3	48	theme	glycans	583:589	arg1	analysis					571:578	the analysis	567:578	the analysis of glycans	567:589	Due to their high structural variability, the analysis of glycans still represents a challenging task.					
30535529	4	49	theme	first	646:650	arg1	coupling					660:667	the first on-line coupling	642:667	the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS)	642:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	8	50	contain	has	1379:1381	arg2	impact					1397:1402	a significant impact	1383:1402	a significant impact on the obtained drift time profile	1383:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	8	50	contain	has	1379:1381	arg1	type					1339:1342	the type	1335:1342	the type of sialic acid attached to glycans	1335:1377	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	8	51	theme	time	1426:1429	arg1	profile					1431:1437	the obtained drift time profile	1407:1437	the obtained drift time profile	1407:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	8	52	theme	obtained	1411:1418	arg1	profile					1431:1437	the obtained drift time profile	1407:1437	the obtained drift time profile	1407:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	1	53	theme	liquid	217:222	arg1	phase					224:228	the liquid phase	213:228	the liquid phase	213:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	0	54	theme	ion	53:55	arg1	spectrometry					71:82	drift tube ion mobility-mass spectrometry	42:82	drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans	42:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	1	55	theme	gas	293:295	arg1	phase					297:301	the gas phase	289:301	the gas phase	289:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	2	56	theme	biological	484:493	arg1	functions					495:503	biological functions	484:503	biological functions	484:503	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	9	57	theme	sialic	1582:1587	arg1	α2-3/α2-6					1604:1612	α2-3/α2-6	1604:1612	α2-3/α2-6	1604:1612	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	9	57	theme	sialic	1582:1587	arg1	linkages					1594:1601	their sialic acid linkages	1576:1601	their sialic acid linkages (α2-3/α2-6)	1576:1613	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	4	58	theme	tube	687:690	arg1	DTIM-MS					724:730	DTIM-MS	724:730	DTIM-MS	724:730	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	58	theme	tube	687:690	arg1	spectrometry					710:721	drift tube ion mobility-mass spectrometry	681:721	drift tube ion mobility-mass spectrometry (DTIM-MS)	681:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	0	59	theme	tube	48:51	arg1	spectrometry					71:82	drift tube ion mobility-mass spectrometry	42:82	drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans	42:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	4	60	theme	N-glycans	877:885	arg1	analysis					802:809	the analysis	798:809	the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	798:885	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	61	theme	native	814:819	arg1	N-glycans					877:885	native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	814:885	native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	814:885	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	1	62	from	mobility	201:208	arg1	phase					224:228	the liquid phase	213:228	the liquid phase	213:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	4	63	theme	drift	681:685	arg1	DTIM-MS					724:730	DTIM-MS	724:730	DTIM-MS	724:730	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	63	theme	drift	681:685	arg1	spectrometry					710:721	drift tube ion mobility-mass spectrometry	681:721	drift tube ion mobility-mass spectrometry (DTIM-MS)	681:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	6	64	theme	IMS	1165:1167	arg1	dimension					1169:1177	the IMS dimension	1161:1177	the IMS dimension	1161:1177	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	8	65	theme	significant	1385:1395	arg1	impact					1397:1402	a significant impact	1383:1402	a significant impact on the obtained drift time profile	1383:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	3	66	theme	challenging	610:620	arg1	task					622:625	a challenging task	608:625	a challenging task	608:625	Due to their high structural variability, the analysis of glycans still represents a challenging task.					
30535529	4	67	theme	mobility-mass	696:708	arg1	DTIM-MS					724:730	DTIM-MS	724:730	DTIM-MS	724:730	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	4	67	theme	mobility-mass	696:708	arg1	spectrometry					710:721	drift tube ion mobility-mass spectrometry	681:721	drift tube ion mobility-mass spectrometry (DTIM-MS)	681:731	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	5	68	theme	signals	924:930	arg1	complexity					903:912	a complexity	901:912	a complexity of glycan signals	901:930	In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans.					
30535529	6	69	located	observed	1149:1156	arg1	dimension					1169:1177	the IMS dimension	1161:1177	the IMS dimension	1161:1177	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	6	69	located	observed	1149:1156	arg2	peaks					1143:1147	peaks	1143:1147	peaks observed in the IMS dimension	1143:1177	Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension.					
30535529	5	70	theme	native	1022:1027	arg1	glycans					1046:1052	both native and APTS-labeled glycans	1017:1052	both native and APTS-labeled glycans	1017:1052	In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans.					
30535529	1	71	theme	Capillary	139:147	arg1	techniques					321:330	both powerful techniques	307:330	both powerful techniques for the separation of complex samples	307:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	71	theme	Capillary	139:147	arg1	electrophoresis					154:168	Capillary zone electrophoresis	139:168	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase	139:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	71	theme	Capillary	139:147	arg1	spectrometry					247:258	ion mobility spectrometry	234:258	ion mobility spectrometry (IMS) based on mobilities in the gas phase	234:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	71	theme	Capillary	139:147	arg1	CZE					171:173	CZE	171:173	CZE	171:173	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	2	72	theme	functions	495:503	arg1	range					475:479	a wide range	468:479	a wide range of biological functions and human diseases	468:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	5	73	dep	resolved	964:971	arg1	shown					1007:1011	shown	1007:1011	shown for both native and APTS-labeled glycans	1007:1052	In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans.					
30535529	8	74	theme	drift	1420:1424	arg1	profile					1431:1437	the obtained drift time profile	1407:1437	the obtained drift time profile	1407:1437	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	1	75	theme	zone	149:152	arg1	techniques					321:330	both powerful techniques	307:330	both powerful techniques for the separation of complex samples	307:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	75	theme	zone	149:152	arg1	electrophoresis					154:168	Capillary zone electrophoresis	139:168	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase	139:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	75	theme	zone	149:152	arg1	spectrometry					247:258	ion mobility spectrometry	234:258	ion mobility spectrometry (IMS) based on mobilities in the gas phase	234:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	75	theme	zone	149:152	arg1	CZE					171:173	CZE	171:173	CZE	171:173	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	8	76	theme	acid	1354:1357	arg1	type					1339:1342	the type	1335:1342	the type of sialic acid attached to glycans	1335:1377	In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile.					
30535529	1	77	theme	ion	234:236	arg1	techniques					321:330	both powerful techniques	307:330	both powerful techniques for the separation of complex samples	307:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	77	theme	ion	234:236	arg1	electrophoresis					154:168	Capillary zone electrophoresis	139:168	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase	139:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	77	theme	ion	234:236	arg1	IMS					261:263	IMS	261:263	IMS	261:263	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	77	theme	ion	234:236	arg1	spectrometry					247:258	ion mobility spectrometry	234:258	ion mobility spectrometry (IMS) based on mobilities in the gas phase	234:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	0	78	theme	mobility-mass	57:69	arg1	spectrometry					71:82	drift tube ion mobility-mass spectrometry	42:82	drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans	42:136	Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.					
30535529	4	79	theme	separation	770:779	arg1	capabilities					781:792	separation capabilities	770:792	separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans	770:885	In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans.					
30535529	2	80	theme	modifications	438:450	arg1	one					396:398	one	396:398	one	396:398	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	2	80	theme	modifications	438:450	arg1	modifications					438:450	the most common post-translational modifications	403:450	the most common post-translational modifications associated with a wide range of biological functions and human diseases	403:522	Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases.					
30535529	9	81	theme	acid	1589:1592	arg1	α2-3/α2-6					1604:1612	α2-3/α2-6	1604:1612	α2-3/α2-6	1604:1612	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	9	81	theme	acid	1589:1592	arg1	linkages					1594:1601	their sialic acid linkages	1576:1601	their sialic acid linkages (α2-3/α2-6)	1576:1613	Furthermore, the application of α2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (α2-3/α2-6).					
30535529	1	82	theme	mobility	238:245	arg1	techniques					321:330	both powerful techniques	307:330	both powerful techniques for the separation of complex samples	307:368	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	82	theme	mobility	238:245	arg1	electrophoresis					154:168	Capillary zone electrophoresis	139:168	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase	139:228	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	82	theme	mobility	238:245	arg1	IMS					261:263	IMS	261:263	IMS	261:263	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30535529	1	82	theme	mobility	238:245	arg1	spectrometry					247:258	ion mobility spectrometry	234:258	ion mobility spectrometry (IMS) based on mobilities in the gas phase	234:301	Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples.					
30075362	5	0	theme	high-mannose	898:909	arg1	oligosaccharides					911:926	M6P-tagged high-mannose oligosaccharides	887:926	M6P-tagged high-mannose oligosaccharides	887:926	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	1	1	theme	M6P	213:215	arg1	receptors					217:225	transmembrane M6P receptors	199:225	transmembrane M6P receptors	199:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	4	2	theme	chemical	660:667	arg1	glycosylation					669:681	Regioselective chemical glycosylation	645:681	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols	645:755	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols were applied to rapidly assemble these oligosaccharides.					
30075362	5	3	theme	oligosaccharides	911:926	arg1	preparation					872:882	the preparation	868:882	the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions	868:1023	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	2	4	theme	recognition	420:430	arg1	process					432:438	the M6P ligand-receptor recognition process	396:438	the M6P ligand-receptor recognition process	396:438	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	4	5	theme	Regioselective	645:658	arg1	glycosylation					669:681	Regioselective chemical glycosylation	645:681	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols	645:755	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols were applied to rapidly assemble these oligosaccharides.					
30075362	5	6	theme	strategy	848:855	arg1	development					818:828	The development	814:828	The development of this synthetic strategy	814:855	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	2	7	theme	process	432:438	arg1	understanding					379:391	a thorough understanding	368:391	a thorough understanding of the M6P ligand-receptor recognition process	368:438	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	4	8	theme	product	726:732	arg1	protocols					747:755	product purification protocols	726:755	product purification protocols	726:755	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols were applied to rapidly assemble these oligosaccharides.					
30075362	3	9	theme	reliable	493:500	arg1	strategy					511:518	a versatile and reliable chemical strategy	477:518	a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	477:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	10	theme	strategy	511:518	arg1	application					462:472	the application	458:472	the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	458:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	11	theme	versatile	479:487	arg1	strategy					511:518	a versatile and reliable chemical strategy	477:518	a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	477:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	1	12	theme	Molecular	119:127	arg1	event					246:250	a key signaling event	230:250	a key signaling event in lysosomal protein trafficking in vivo	230:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	1	12	theme	Molecular	119:127	arg1	recognition					129:139	Molecular recognition	119:139	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors	119:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	5	13	theme	biological	1004:1013	arg1	functions					1015:1023	biological functions	1004:1023	biological functions	1004:1023	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	3	14	from	oligosaccharides	579:594	arg1	purity					630:635	high purity	625:635	high purity (>98%)	625:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	14	from	oligosaccharides	579:594	arg1	%					641:641	>98%	638:641	>98%	638:641	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	14	from	oligosaccharides	579:594	arg1	yield					612:616	>20% overall yield	599:616	>20% overall yield	599:616	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	1	15	theme	key	232:234	arg1	recognition					129:139	Molecular recognition	119:139	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors	119:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	1	15	theme	key	232:234	arg1	event					246:250	a key signaling event	230:250	a key signaling event in lysosomal protein trafficking in vivo	230:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	2	16	theme	thorough	370:377	arg1	understanding					379:391	a thorough understanding	368:391	a thorough understanding of the M6P ligand-receptor recognition process	368:438	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	1	17	theme	signaling	236:244	arg1	recognition					129:139	Molecular recognition	119:139	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors	119:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	1	17	theme	signaling	236:244	arg1	event					246:250	a key signaling event	230:250	a key signaling event in lysosomal protein trafficking in vivo	230:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	0	18	theme	oligosaccharides	26:41	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of high-mannose oligosaccharides	0:41	Synthesis of high-mannose oligosaccharides containing mannose-6-phosphate residues using regioselective glycosylation.					
30075362	3	19	theme	di-antennary	542:553	arg1	oligosaccharides					579:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides	531:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	531:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	0	20	theme	high-mannose	13:24	arg1	oligosaccharides					26:41	high-mannose oligosaccharides	13:41	high-mannose oligosaccharides	13:41	Synthesis of high-mannose oligosaccharides containing mannose-6-phosphate residues using regioselective glycosylation.					
30075362	3	21	theme	overall	604:610	arg1	yield					612:616	>20% overall yield	599:616	>20% overall yield	599:616	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	22	theme	M6P-tagged	555:564	arg1	oligosaccharides					579:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides	531:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	531:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	0	23	theme	mannose-6-phosphate	54:72	arg1	residues					74:81	mannose-6-phosphate residues	54:81	mannose-6-phosphate residues using regioselective glycosylation	54:116	Synthesis of high-mannose oligosaccharides containing mannose-6-phosphate residues using regioselective glycosylation.					
30075362	3	24	theme	>20	599:601	arg1	%					602:602	%	602:602	%	602:602	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	25	theme	chemical	502:509	arg1	strategy					511:518	a versatile and reliable chemical strategy	477:518	a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	477:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	1	26	theme	lysosomal	255:263	arg1	trafficking					273:283	lysosomal protein trafficking	255:283	lysosomal protein trafficking in vivo	255:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	5	27	theme	synthetic	838:846	arg1	strategy					848:855	this synthetic strategy	833:855	this synthetic strategy	833:855	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	1	28	theme	protein	265:271	arg1	trafficking					273:283	lysosomal protein trafficking	255:283	lysosomal protein trafficking in vivo	255:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	3	29	theme	%	602:602	arg1	yield					612:616	>20% overall yield	599:616	>20% overall yield	599:616	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	5	30	theme	M6P-tagged	887:896	arg1	oligosaccharides					911:926	M6P-tagged high-mannose oligosaccharides	887:926	M6P-tagged high-mannose oligosaccharides	887:926	The development of this synthetic strategy simplifies the preparation of M6P-tagged high-mannose oligosaccharides, which will improve access to these compounds to study their structures and biological functions.					
30075362	2	31	theme	ligand-receptor	404:418	arg1	process					432:438	the M6P ligand-receptor recognition process	396:438	the M6P ligand-receptor recognition process	396:438	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	1	32	from	event	246:250	arg1	trafficking					273:283	lysosomal protein trafficking	255:283	lysosomal protein trafficking in vivo	255:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	2	33	theme	M6P	400:402	arg1	process					432:438	the M6P ligand-receptor recognition process	396:438	the M6P ligand-receptor recognition process	396:438	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	2	34	theme	high-mannose	319:330	arg1	N-glycans					332:340	M6P-containing high-mannose N-glycans	304:340	M6P-containing high-mannose N-glycans	304:340	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	1	35	theme	-modified	169:177	arg1	oligosaccharides					179:194	mannose-6-phosphate (M6P)-modified oligosaccharides	144:194	mannose-6-phosphate (M6P)-modified oligosaccharides	144:194	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	4	36	theme	effective	696:704	arg1	phosphorylation					706:720	effective phosphorylation	696:720	effective phosphorylation	696:720	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols were applied to rapidly assemble these oligosaccharides.					
30075362	3	37	theme	high	625:628	arg1	purity					630:635	high purity	625:635	high purity (>98%)	625:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	37	theme	high	625:628	arg1	%					641:641	>98%	638:641	>98%	638:641	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	2	38	theme	M6P-containing	304:317	arg1	N-glycans					332:340	M6P-containing high-mannose N-glycans	304:340	M6P-containing high-mannose N-glycans	304:340	Access to M6P-containing high-mannose N-glycans is essential to achieving a thorough understanding of the M6P ligand-receptor recognition process.					
30075362	1	39	theme	oligosaccharides	179:194	arg1	event					246:250	a key signaling event	230:250	a key signaling event in lysosomal protein trafficking in vivo	230:291	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	1	39	theme	oligosaccharides	179:194	arg1	recognition					129:139	Molecular recognition	119:139	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors	119:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
30075362	3	40	theme	high-mannose	566:577	arg1	oligosaccharides					579:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides	531:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	531:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	3	41	theme	asymmetric	531:540	arg1	oligosaccharides					579:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides	531:594	asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%)	531:642	Herein we report the application of a versatile and reliable chemical strategy to prepare asymmetric di-antennary M6P-tagged high-mannose oligosaccharides in >20% overall yield and in high purity (>98%).					
30075362	4	42	theme	purification	734:745	arg1	protocols					747:755	product purification protocols	726:755	product purification protocols	726:755	Regioselective chemical glycosylation coupled with effective phosphorylation and product purification protocols were applied to rapidly assemble these oligosaccharides.					
30075362	0	43	theme	regioselective	89:102	arg1	glycosylation					104:116	regioselective glycosylation	89:116	regioselective glycosylation	89:116	Synthesis of high-mannose oligosaccharides containing mannose-6-phosphate residues using regioselective glycosylation.					
30075362	1	44	theme	transmembrane	199:211	arg1	receptors					217:225	transmembrane M6P receptors	199:225	transmembrane M6P receptors	199:225	Molecular recognition of mannose-6-phosphate (M6P)-modified oligosaccharides by transmembrane M6P receptors is a key signaling event in lysosomal protein trafficking in vivo.					
29727657	0	0	theme	Chitinophaga	82:93	arg1	pinensis					95:102	Chitinophaga pinensis	82:102	Chitinophaga pinensis	82:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	9	1	theme	chitin	1451:1456	arg1	waste					1458:1462	chitin waste	1451:1462	chitin waste	1451:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	1	2	from	0.133	348:352	arg1	chitin					381:386	colloidal chitin	371:386	colloidal chitin	371:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	4	3	theme	tertiary	734:741	arg1	structures					743:752	the secondary and tertiary structures	716:752	the secondary and tertiary structures of CpChiC-GH18C	716:768	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	4	3	theme	tertiary	734:741	arg1	unaltered					774:782	unaltered	774:782	unaltered	774:782	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	1	4	theme	chitinase	175:183	arg1	C					185:185	chitinase C	175:185	chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	175:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	9	5	theme	open	1322:1325	arg1	groove					1327:1332	a shallow and open groove	1308:1332	a shallow and open groove	1308:1332	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	7	6	theme	p-hydroxymercuribenzoic	1069:1091	arg1	modulators					1057:1066	The chemical modulators	1044:1066	The chemical modulators	1044:1066	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	7	6	theme	p-hydroxymercuribenzoic	1069:1091	arg1	acid					1093:1096	p-hydroxymercuribenzoic acid	1069:1096	p-hydroxymercuribenzoic acid	1069:1096	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	2	7	theme	degradation	401:411	arg1	Analysis					389:396	Analysis	389:396	Analysis of degradation of (GlcNAc)3-6 oligomers	389:436	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	9	8	contain	has	1304:1306	arg1	CpChiC-GH18C					1291:1302	the CpChiC-GH18C	1287:1302	the CpChiC-GH18C	1287:1302	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	9	8	contain	has	1304:1306	arg2	groove					1327:1332	a shallow and open groove	1308:1332	a shallow and open groove	1308:1332	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	0	9	theme	pinensis	95:102	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	1	10	theme	10.16	318:322	arg1	Km					285:286	a Km	283:286	a Km of 76.13 (mg-1 ml)	283:305	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	10	theme	10.16	318:322	arg1	kcat					310:313	a kcat	308:313	a kcat of 10.16 (s-1)	308:328	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	10	theme	10.16	318:322	arg1	kcat/Km					337:343	a kcat/Km	335:343	a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin	335:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	2	11	theme	main	488:491	arg1	product					493:499	the main product	484:499	the main product	484:499	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	1	12	theme	colloidal	371:379	arg1	chitin					381:386	colloidal chitin	371:386	colloidal chitin	371:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	0	13	theme	non-processive	109:122	arg1	exochitinase					124:135	a non-processive exochitinase	107:135	a non-processive exochitinase	107:135	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	13	theme	non-processive	109:122	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	13	theme	non-processive	109:122	arg1	domain					39:44	Carboxy-terminal glycosyl hydrolase 18 domain	0:44	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis	0:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	1	14	from	Km	285:286	arg1	chitin					381:386	colloidal chitin	371:386	colloidal chitin	371:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	8	15	theme	Sequence	1164:1171	arg1	analysis					1173:1180	Sequence analysis	1164:1180	Sequence analysis	1164:1180	Sequence analysis and homology modeling suggest that CpChiC-GH18C lacks a tryptophan residue at the aglycon site.					
29727657	1	16	theme	C	185:185	arg1	domain					165:170	The recombinant C-terminal domain	138:170	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	138:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	16	theme	C	185:185	arg1	C					185:185	chitinase C	175:185	chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	175:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	4	17	dep	45 °C	790:794	arg1	up					784:785	up	784:785	up	784:785	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	2	18	theme	cleavage	525:532	arg1	pattern					534:540	an exo-type cleavage pattern	513:540	an exo-type cleavage pattern	513:540	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	5	19	theme	urea-induced	857:868	arg1	unfolding					870:878	The urea-induced unfolding	853:878	The urea-induced unfolding	853:878	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	5	19	theme	urea-induced	857:868	arg1	process					895:901	a two-state process	883:901	a two-state process	883:901	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	2	20	theme	oligomers	428:436	arg1	degradation					401:411	degradation	401:411	degradation of (GlcNAc)3-6 oligomers	401:436	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	0	21	theme	glycosyl	17:24	arg1	exochitinase					124:135	a non-processive exochitinase	107:135	a non-processive exochitinase	107:135	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	21	theme	glycosyl	17:24	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	21	theme	glycosyl	17:24	arg1	domain					39:44	Carboxy-terminal glycosyl hydrolase 18 domain	0:44	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis	0:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	2	22	theme	exo-type	516:523	arg1	pattern					534:540	an exo-type cleavage pattern	513:540	an exo-type cleavage pattern	513:540	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	6	23	theme	enzyme	1027:1032	arg1	activity					1034:1041	the enzyme activity	1023:1041	the enzyme activity	1023:1041	Among the metal ions tested, Hg2+ completely inhibits the enzyme activity.					
29727657	1	24	theme	Chitinophaga	190:201	arg1	CpChiC-GH18C					213:224	CpChiC-GH18C	213:224	CpChiC-GH18C	213:224	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	24	theme	Chitinophaga	190:201	arg1	pinensis					203:210	Chitinophaga pinensis	190:210	Chitinophaga pinensis (CpChiC-GH18C)	190:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	0	25	theme	Carboxy-terminal	0:15	arg1	exochitinase					124:135	a non-processive exochitinase	107:135	a non-processive exochitinase	107:135	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	25	theme	Carboxy-terminal	0:15	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	25	theme	Carboxy-terminal	0:15	arg1	domain					39:44	Carboxy-terminal glycosyl hydrolase 18 domain	0:44	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis	0:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	4	26	theme	secondary	720:728	arg1	structures					743:752	the secondary and tertiary structures	716:752	the secondary and tertiary structures of CpChiC-GH18C	716:768	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	4	26	theme	secondary	720:728	arg1	unaltered					774:782	unaltered	774:782	unaltered	774:782	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	1	27	theme	pinensis	203:210	arg1	C					185:185	chitinase C	175:185	chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	175:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	28	from	35 °C	271:275	arg1	activity					248:255	the highest activity	236:255	the highest activity at pH 6.0 and 35 °C	236:275	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	8	29	theme	tryptophan	1238:1247	arg1	residue					1249:1255	a tryptophan residue	1236:1255	a tryptophan residue at the aglycon site	1236:1275	Sequence analysis and homology modeling suggest that CpChiC-GH18C lacks a tryptophan residue at the aglycon site.					
29727657	9	30	theme	non-processive	1367:1380	arg1	chitinase					1391:1399	non-processive exo-type chitinase	1367:1399	non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste	1367:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	0	31	theme	hydrolase	26:34	arg1	exochitinase					124:135	a non-processive exochitinase	107:135	a non-processive exochitinase	107:135	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	31	theme	hydrolase	26:34	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	31	theme	hydrolase	26:34	arg1	domain					39:44	Carboxy-terminal glycosyl hydrolase 18 domain	0:44	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis	0:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	7	32	theme	enzyme	1147:1152	arg1	activity					1154:1161	the enzyme activity	1143:1161	the enzyme activity	1143:1161	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	9	33	theme	waste	1458:1462	arg1	bioconversion					1434:1446	the bioconversion	1430:1446	the bioconversion of chitin waste	1430:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	6	34	theme	metal	979:983	arg1	ions					985:988	the metal ions	975:988	the metal ions tested	975:995	Among the metal ions tested, Hg2+ completely inhibits the enzyme activity.					
29727657	1	35	from	kcat	310:313	arg1	chitin					381:386	colloidal chitin	371:386	colloidal chitin	371:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	9	36	theme	suitable	1417:1424	arg1	properties					1406:1415	properties	1406:1415	properties suitable for the bioconversion of chitin waste	1406:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	1	37	from	pH 6.0	260:265	arg1	activity					248:255	the highest activity	236:255	the highest activity at pH 6.0 and 35 °C	236:275	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	7	38	theme	chemical	1048:1055	arg1	N-bromosuccinimide					1102:1119	N-bromosuccinimide	1102:1119	N-bromosuccinimide	1102:1119	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	7	38	theme	chemical	1048:1055	arg1	modulators					1057:1066	The chemical modulators	1044:1066	The chemical modulators	1044:1066	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	7	38	theme	chemical	1048:1055	arg1	acid					1093:1096	p-hydroxymercuribenzoic acid	1069:1096	p-hydroxymercuribenzoic acid	1069:1096	The chemical modulators, p-hydroxymercuribenzoic acid and N-bromosuccinimide considerably decrease the enzyme activity.					
29727657	4	39	theme	dichroism	684:692	arg1	spectra					694:700	Circular dichroism spectra	675:700	Circular dichroism spectra	675:700	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	8	40	theme	homology	1186:1193	arg1	modeling					1195:1202	homology modeling	1186:1202	homology modeling	1186:1202	Sequence analysis and homology modeling suggest that CpChiC-GH18C lacks a tryptophan residue at the aglycon site.					
29727657	8	41	from	site	1272:1275	arg1	residue					1249:1255	a tryptophan residue	1236:1255	a tryptophan residue at the aglycon site	1236:1275	Sequence analysis and homology modeling suggest that CpChiC-GH18C lacks a tryptophan residue at the aglycon site.					
29727657	1	42	theme	76.13	291:295	arg1	Km					285:286	a Km	283:286	a Km of 76.13 (mg-1 ml)	283:305	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	42	theme	76.13	291:295	arg1	kcat					310:313	a kcat	308:313	a kcat of 10.16 (s-1)	308:328	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	42	theme	76.13	291:295	arg1	kcat/Km					337:343	a kcat/Km	335:343	a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin	335:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	5	43	theme	single	956:961	arg1	step					963:966	a single step	954:966	a single step	954:966	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	4	44	theme	Circular	675:682	arg1	spectra					694:700	Circular dichroism spectra	675:700	Circular dichroism spectra	675:700	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	0	45	theme	carbohydrate	51:62	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	2	46	theme	GlcNAc	417:422	arg1	oligomers					428:436	(GlcNAc)3-6 oligomers	416:436	(GlcNAc)3-6 oligomers	416:436	Analysis of degradation of (GlcNAc)3-6 oligomers shows that CpChiC-GH18C releases (GlcNAc)2 as the main product, indicating an exo-type cleavage pattern.					
29727657	3	47	theme	end	632:634	arg1	GlcNAc					605:610	(GlcNAc)2	604:612	(GlcNAc)2	604:612	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	3	47	theme	end	632:634	arg1	products					636:643	end products	632:643	end products	632:643	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	3	47	theme	end	632:634	arg1	GlcNAc					620:625	(GlcNAc)3	619:627	(GlcNAc)3	619:627	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	3	47	theme	end	632:634	arg1	GlcNAc					596:601	GlcNAc	596:601	GlcNAc	596:601	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	9	48	with	chitinase	1391:1399	arg1	properties					1406:1415	properties	1406:1415	properties suitable for the bioconversion of chitin waste	1406:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	5	49	theme	native	924:929	arg1	CpChiC-GH18C					931:942	native CpChiC-GH18C	924:942	native CpChiC-GH18C	924:942	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	9	50	theme	exo-type	1382:1389	arg1	chitinase					1391:1399	non-processive exo-type chitinase	1367:1399	non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste	1367:1462	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	4	51	theme	CpChiC-GH18C	757:768	arg1	structures					743:752	the secondary and tertiary structures	716:752	the secondary and tertiary structures of CpChiC-GH18C	716:768	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	4	51	theme	CpChiC-GH18C	757:768	arg1	unaltered					774:782	unaltered	774:782	unaltered	774:782	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	4	52	theme	intermediate	833:844	arg1	state					846:850	an intermediate state	830:850	an intermediate state	830:850	Circular dichroism spectra indicate that the secondary and tertiary structures of CpChiC-GH18C are unaltered up to 45 °C and the protein denatures without an intermediate state.					
29727657	1	53	theme	0.133	348:352	arg1	Km					285:286	a Km	283:286	a Km of 76.13 (mg-1 ml)	283:305	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	53	theme	0.133	348:352	arg1	kcat					310:313	a kcat	308:313	a kcat of 10.16 (s-1)	308:328	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	53	theme	0.133	348:352	arg1	kcat/Km					337:343	a kcat/Km	335:343	a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin	335:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	3	54	theme	processivity	661:672	arg1	sign					653:656	no sign	650:656	no sign of processivity	650:672	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	8	55	theme	aglycon	1264:1270	arg1	site					1272:1275	the aglycon site	1260:1275	the aglycon site	1260:1275	Sequence analysis and homology modeling suggest that CpChiC-GH18C lacks a tryptophan residue at the aglycon site.					
29727657	3	56	theme	chitin	571:576	arg1	polymers					578:585	the chitin polymers	567:585	the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products	567:643	CpChiC-GH18C hydrolyzes the chitin polymers yielding GlcNAc, (GlcNAc)2, and (GlcNAc)3 as end products with no sign of processivity.					
29727657	1	57	theme	recombinant	142:152	arg1	domain					165:170	The recombinant C-terminal domain	138:170	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	138:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	57	theme	recombinant	142:152	arg1	C					185:185	chitinase C	175:185	chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	175:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	5	58	theme	CpChiC-GH18C	931:942	arg1	unfolding					911:919	the unfolding	907:919	the unfolding of native CpChiC-GH18C	907:942	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	1	59	theme	highest	240:246	arg1	activity					248:255	the highest activity	236:255	the highest activity at pH 6.0 and 35 °C	236:275	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	0	60	theme	protein	71:77	arg1	exochitinase					124:135	a non-processive exochitinase	107:135	a non-processive exochitinase	107:135	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	60	theme	protein	71:77	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	0	60	theme	protein	71:77	arg1	domain					39:44	Carboxy-terminal glycosyl hydrolase 18 domain	0:44	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis	0:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	1	61	theme	C-terminal	154:163	arg1	domain					165:170	The recombinant C-terminal domain	138:170	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	138:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	61	theme	C-terminal	154:163	arg1	C					185:185	chitinase C	175:185	chitinase C of Chitinophaga pinensis (CpChiC-GH18C)	175:225	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	0	62	theme	active	64:69	arg1	protein					71:77	a carbohydrate active protein	49:77	a carbohydrate active protein of Chitinophaga pinensis	49:102	Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.					
29727657	5	63	theme	two-state	885:893	arg1	unfolding					870:878	The urea-induced unfolding	853:878	The urea-induced unfolding	853:878	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	5	63	theme	two-state	885:893	arg1	process					895:901	a two-state process	883:901	a two-state process	883:901	The urea-induced unfolding is a two-state process and the unfolding of native CpChiC-GH18C occurs in a single step.					
29727657	1	64	from	chitin	381:386	arg1	Km					285:286	a Km	283:286	a Km of 76.13 (mg-1 ml)	283:305	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	64	from	chitin	381:386	arg1	kcat					310:313	a kcat	308:313	a kcat of 10.16 (s-1)	308:328	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	1	64	from	chitin	381:386	arg1	kcat/Km					337:343	a kcat/Km	335:343	a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin	335:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29727657	9	65	theme	shallow	1310:1316	arg1	groove					1327:1332	a shallow and open groove	1308:1332	a shallow and open groove	1308:1332	Further, the CpChiC-GH18C has a shallow and open groove, suggesting that CpChiC-GH18C is non-processive exo-type chitinase with properties suitable for the bioconversion of chitin waste.					
29727657	1	66	from	kcat/Km	337:343	arg1	chitin					381:386	colloidal chitin	371:386	colloidal chitin	371:386	The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18C) exhibits the highest activity at pH 6.0 and 35 °C, with a Km of 76.13 (mg-1 ml), a kcat of 10.16 (s-1), and a kcat/Km of 0.133 (mg-1 ml s-1) on colloidal chitin.					
29518231	4	0	theme	publication	522:532	arg1	images					534:539	publication images	522:539	publication images	522:539	Thus, results end up being encoded in publication images and text, while valuable curated data is stored in files as supplementary information.					
29518231	8	1	theme	Glycan	972:977	arg1	structures					979:988	Glycan structures	972:988	Glycan structures of interest	972:1000	Glycan structures of interest can then be sent to EpitopeXtractor to perform a glycoepitope extraction.					
29518231	2	2	theme	large	350:354	arg1	amount					356:361	large amount	350:361	large amount of information	350:376	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	2	2	theme	large	350:354	arg1	information					366:376	information	366:376	information	366:376	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	7	3	theme	Several	860:866	arg1	profiles					868:875	Several profiles	860:875	Several profiles	860:875	Several profiles can be compared and glycan composition is integrated with structural data stored in databases.					
29518231	10	4	theme	glycoepitopes	1220:1232	arg1	clusters					1208:1215	clusters	1208:1215	clusters of glycoepitopes and discovery of potential new targets	1208:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	8	5	theme	interest	993:1000	arg1	structures					979:988	Glycan structures	972:988	Glycan structures of interest	972:1000	Glycan structures of interest can then be sent to EpitopeXtractor to perform a glycoepitope extraction.					
29518231	10	6	theme	targets	1265:1271	arg1	discovery					1238:1246	discovery	1238:1246	discovery of potential new targets	1238:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	10	6	theme	targets	1265:1271	arg1	glycoepitopes					1220:1232	glycoepitopes	1220:1232	glycoepitopes	1220:1232	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	12	7	theme	ExPASy	1480:1485	arg1	initiative					1487:1496	the Glycomics@ExPASy initiative	1466:1496	the Glycomics@ExPASy initiative	1466:1496	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	12	8	from	https	1519:1523	arg1	available					1506:1514	available	1506:1514	available	1506:1514	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	12	8	from	https	1519:1523	arg1	part					1458:1461	part	1458:1461	part of the Glycomics@ExPASy initiative	1458:1496	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	12	8	from	https	1519:1523	arg1	tools					1424:1428	All the tools	1416:1428	All the tools composing this pipeline	1416:1452	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	10	9	theme	clusters	1208:1215	arg1	detection					1195:1203	fast detection	1190:1203	fast detection of clusters of glycoepitopes and discovery of potential new targets	1190:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	11	10	theme	specific	1385:1392	arg1	interest					1394:1401	the specific interest	1381:1401	the specific interest of the user	1381:1413	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	0	11	from	view	42:45	arg1	glycocompositions					69:85	glycocompositions	69:85	glycocompositions to glycoepitopes	69:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	2	12	theme	data	274:277	arg1	growth					247:252	The rapid growth	237:252	The rapid growth of available glycan data	237:277	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	9	13	theme	EpitopeXtractor	1076:1090	arg1	results					1092:1098	EpitopeXtractor results	1076:1098	EpitopeXtractor results	1076:1098	EpitopeXtractor results can be superimposed on the Glydin' glycoepitope network.					
29518231	3	14	theme	ad	457:458	arg1	visualization					469:481	ad hoc data visualization	457:481	ad hoc data visualization	457:481	Scientists resort to using general-purpose spreadsheet applications to create ad hoc data visualization.					
29518231	3	15	theme	spreadsheet	422:432	arg1	applications					434:445	general-purpose spreadsheet applications	406:445	general-purpose spreadsheet applications	406:445	Scientists resort to using general-purpose spreadsheet applications to create ad hoc data visualization.					
29518231	2	16	theme	glycan	267:272	arg1	data					274:277	available glycan data	257:277	available glycan data	257:277	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	11	17	theme	user	1410:1413	arg1	interest					1394:1401	the specific interest	1381:1401	the specific interest of the user	1381:1413	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	11	17	theme	user	1410:1413	arg1	data					1372:1375	the data	1368:1375	the data	1368:1375	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	3	18	theme	hoc	460:462	arg1	visualization					469:481	ad hoc data visualization	457:481	ad hoc data visualization	457:481	Scientists resort to using general-purpose spreadsheet applications to create ad hoc data visualization.					
29518231	7	19	theme	structural	935:944	arg1	data					946:949	structural data	935:949	structural data stored in databases	935:969	Several profiles can be compared and glycan composition is integrated with structural data stored in databases.					
29518231	2	20	theme	tools	314:318	arg1	lack					295:298	the lack	291:298	the lack of innovative tools	291:318	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	1	21	theme	large	175:179	arg1	collections					181:191	large collections	175:191	large collections of glycan profiles	175:210	Nowadays, due to the advance of experimental techniques in glycomics, large collections of glycan profiles are regularly published.					
29518231	4	22	theme	valuable	557:564	arg1	data					574:577	valuable curated data	557:577	valuable curated data	557:577	Thus, results end up being encoded in publication images and text, while valuable curated data is stored in files as supplementary information.					
29518231	8	23	theme	glycoepitope	1051:1062	arg1	extraction					1064:1073	a glycoepitope extraction	1049:1073	a glycoepitope extraction	1049:1073	Glycan structures of interest can then be sent to EpitopeXtractor to perform a glycoepitope extraction.					
29518231	2	24	theme	innovative	303:312	arg1	tools					314:318	innovative tools	303:318	innovative tools	303:318	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	12	25	theme	Glycomics	1470:1478	arg1	initiative					1487:1496	the Glycomics@ExPASy initiative	1466:1496	the Glycomics@ExPASy initiative	1466:1496	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	6	26	theme	profile	764:770	arg1	data					772:775	Glycan profile data	757:775	Glycan profile data	757:775	Glycan profile data can be imported in Glynsight, which generates a custom interactive glycan profile.					
29518231	11	27	with	conjunction	1326:1336	arg1	others					1347:1352	the others	1343:1352	the others	1343:1352	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	12	28	theme	@	1479:1479	arg1	initiative					1487:1496	the Glycomics@ExPASy initiative	1466:1496	the Glycomics@ExPASy initiative	1466:1496	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	1	29	theme	glycan	196:201	arg1	profiles					203:210	glycan profiles	196:210	glycan profiles	196:210	Nowadays, due to the advance of experimental techniques in glycomics, large collections of glycan profiles are regularly published.					
29518231	0	30	theme	interactive	30:40	arg1	view					42:45	an interactive view	27:45	an interactive view of glycosylation from glycocompositions to glycoepitopes	27:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	4	31	theme	curated	566:572	arg1	data					574:577	valuable curated data	557:577	valuable curated data	557:577	Thus, results end up being encoded in publication images and text, while valuable curated data is stored in files as supplementary information.					
29518231	0	32	from	glycocompositions	69:85	arg1	glycosylation					50:62	glycosylation	50:62	glycosylation from glycocompositions to glycoepitopes	50:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	0	32	from	glycocompositions	69:85	arg1	view					42:45	an interactive view	27:45	an interactive view of glycosylation from glycocompositions to glycoepitopes	27:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	1	33	theme	profiles	203:210	arg1	collections					181:191	large collections	175:191	large collections of glycan profiles	175:210	Nowadays, due to the advance of experimental techniques in glycomics, large collections of glycan profiles are regularly published.					
29518231	10	34	theme	new	1261:1263	arg1	targets					1265:1271	potential new targets	1251:1271	potential new targets	1251:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	6	35	theme	glycan	844:849	arg1	profile					851:857	a custom interactive glycan profile	823:857	a custom interactive glycan profile	823:857	Glycan profile data can be imported in Glynsight, which generates a custom interactive glycan profile.					
29518231	6	36	theme	Glycan	757:762	arg1	data					772:775	Glycan profile data	757:775	Glycan profile data	757:775	Glycan profile data can be imported in Glynsight, which generates a custom interactive glycan profile.					
29518231	10	37	theme	network	1161:1167	arg1	visualization					1169:1181	The network visualization	1157:1181	The network visualization	1157:1181	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	6	38	theme	interactive	832:842	arg1	profile					851:857	a custom interactive glycan profile	823:857	a custom interactive glycan profile	823:857	Glycan profile data can be imported in Glynsight, which generates a custom interactive glycan profile.					
29518231	3	39	theme	general-purpose	406:420	arg1	applications					434:445	general-purpose spreadsheet applications	406:445	general-purpose spreadsheet applications	406:445	Scientists resort to using general-purpose spreadsheet applications to create ad hoc data visualization.					
29518231	11	40	used	used	1318:1321	arg2	Each					1274:1277	Each	1274:1277	Each	1274:1277	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	11	40	used	used	1318:1321	arg2	tools					1288:1292	these tools	1282:1292	these tools	1282:1292	Each of these tools is standalone or can be used in conjunction with the others, depending on the data and the specific interest of the user.					
29518231	5	41	theme	interactive	669:679	arg1	pipeline					681:688	an interactive pipeline	666:688	an interactive pipeline composed with three tools: Glynsight, EpitopeXtractor and Glydin'	666:754	To tackle this problem, we have built an interactive pipeline composed with three tools: Glynsight, EpitopeXtractor and Glydin'.					
29518231	6	42	theme	custom	825:830	arg1	profile					851:857	a custom interactive glycan profile	823:857	a custom interactive glycan profile	823:857	Glycan profile data can be imported in Glynsight, which generates a custom interactive glycan profile.					
29518231	9	43	theme	glycoepitope	1135:1146	arg1	network					1148:1154	the Glydin' glycoepitope network	1123:1154	the Glydin' glycoepitope network	1123:1154	EpitopeXtractor results can be superimposed on the Glydin' glycoepitope network.					
29518231	12	44	theme	initiative	1487:1496	arg1	available					1506:1514	available	1506:1514	available	1506:1514	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	12	44	theme	initiative	1487:1496	arg1	part					1458:1461	part	1458:1461	part of the Glycomics@ExPASy initiative	1458:1496	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	12	44	theme	initiative	1487:1496	arg1	tools					1424:1428	All the tools	1416:1428	All the tools composing this pipeline	1416:1452	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	7	45	theme	glycan	897:902	arg1	composition					904:914	glycan composition	897:914	glycan composition	897:914	Several profiles can be compared and glycan composition is integrated with structural data stored in databases.					
29518231	0	46	dep	Understanding	0:12	arg1	view					42:45	an interactive view	27:45	an interactive view of glycosylation from glycocompositions to glycoepitopes	27:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	2	47	theme	available	257:265	arg1	data					274:277	available glycan data	257:277	available glycan data	257:277	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	10	48	theme	potential	1251:1259	arg1	targets					1265:1271	potential new targets	1251:1271	potential new targets	1251:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	4	49	theme	supplementary	601:613	arg1	information					615:625	supplementary information	601:625	supplementary information	601:625	Thus, results end up being encoded in publication images and text, while valuable curated data is stored in files as supplementary information.					
29518231	9	50	theme	Glydin	1127:1132	arg1	network					1148:1154	the Glydin' glycoepitope network	1123:1154	the Glydin' glycoepitope network	1123:1154	EpitopeXtractor results can be superimposed on the Glydin' glycoepitope network.					
29518231	12	51	dep	https	1519:1523	arg1	//www.expasy.org/glycomics					1525:1550	//www.expasy.org/glycomics	1525:1550	https://www.expasy.org/glycomics	1519:1550	All the tools composing this pipeline are part of the Glycomics@ExPASy initiative and are available at https://www.expasy.org/glycomics.					
29518231	3	52	theme	data	464:467	arg1	visualization					469:481	ad hoc data visualization	457:481	ad hoc data visualization	457:481	Scientists resort to using general-purpose spreadsheet applications to create ad hoc data visualization.					
29518231	2	53	theme	information	366:376	arg1	amount					356:361	large amount	350:361	large amount of information	350:376	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	2	53	theme	information	366:376	arg1	information					366:376	information	366:376	information	366:376	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	10	54	theme	discovery	1238:1246	arg1	clusters					1208:1215	clusters	1208:1215	clusters of glycoepitopes and discovery of potential new targets	1208:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	10	55	theme	fast	1190:1193	arg1	detection					1195:1203	fast detection	1190:1203	fast detection of clusters of glycoepitopes and discovery of potential new targets	1190:1271	The network visualization allows fast detection of clusters of glycoepitopes and discovery of potential new targets.					
29518231	0	56	theme	glycosylation	50:62	arg1	view					42:45	an interactive view	27:45	an interactive view of glycosylation from glycocompositions to glycoepitopes	27:102	Understanding the glycome: an interactive view of glycosylation from glycocompositions to glycoepitopes.					
29518231	2	57	theme	rapid	241:245	arg1	growth					247:252	The rapid growth	237:252	The rapid growth of available glycan data	237:277	The rapid growth of available glycan data accentuates the lack of innovative tools for visualizing and exploring large amount of information.					
29518231	1	58	theme	experimental	137:148	arg1	techniques					150:159	experimental techniques	137:159	experimental techniques	137:159	Nowadays, due to the advance of experimental techniques in glycomics, large collections of glycan profiles are regularly published.					
30732783	5	0	theme	dicarbonyl	1096:1105	arg1	compounds					1107:1115	dicarbonyl compounds	1096:1115	dicarbonyl compounds	1096:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	1	theme	inhibitory	878:887	arg1	effect					889:894	the inhibitory effect	874:894	the inhibitory effect of AAPs on AGEs formation	874:920	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	2	from	effects	1026:1032	arg1	group					1055:1059	the protein thiol group	1037:1059	the protein thiol group	1037:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	3	theme	compounds	1107:1115	arg1	capacity					1084:1091	the scavenging capacity	1069:1091	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	0	4	theme	antioxidant	100:110	arg1	activities					129:138	their antioxidant and antiglycated activities	94:138	their antioxidant and antiglycated activities	94:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	5	from	Characterization	0:15	arg1	assessment					80:89	assessment	80:89	assessment of their antioxidant and antiglycated activities	80:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	5	from	Characterization	0:15	arg1	fruit					50:54	fruit	50:54	fruit of Actinidia arguta	50:74	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	6	theme	antiglycated	1152:1163	arg1	effects					1165:1171	antiglycated effects	1152:1171	antiglycated effects	1152:1171	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	2	7	theme	starch-like	549:559	arg1	polysaccharides					561:575	starch-like polysaccharides	549:575	starch-like polysaccharides	549:575	The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides.					
30732783	5	8	theme	inhibitory	943:952	arg1	activity					954:961	the inhibitory activity	939:961	the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	939:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	9	theme	AGEs	907:910	arg1	formation					912:920	AGEs formation	907:920	AGEs formation	907:920	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	0	10	theme	antiglycated	116:127	arg1	activities					129:138	their antioxidant and antiglycated activities	94:138	their antioxidant and antiglycated activities	94:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	4	11	theme	antioxidant	819:829	arg1	activities					849:858	antioxidant and anti-glycated activities	819:858	the strongest antioxidant and anti-glycated activities	805:858	Especially SPS-3 displayed the strongest antioxidant and anti-glycated activities.					
30732783	2	12	theme	monosaccharide	372:385	arg1	composition					387:397	The monosaccharide composition	368:397	The monosaccharide composition	368:397	The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides.					
30732783	0	13	from	fruit	50:54	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.	0:139	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	13	from	fruit	50:54	arg1	fractions					35:43	polysaccharide fractions	20:43	polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities	20:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	4	14	theme	strongest	809:817	arg1	activities					849:858	antioxidant and anti-glycated activities	819:858	the strongest antioxidant and anti-glycated activities	805:858	Especially SPS-3 displayed the strongest antioxidant and anti-glycated activities.					
30732783	3	15	theme	lipid	687:691	arg1	peroxidation					693:704	lipid peroxidation	687:704	lipid peroxidation	687:704	All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS.					
30732783	4	16	theme	anti-glycated	835:847	arg1	activities					849:858	antioxidant and anti-glycated activities	819:858	the strongest antioxidant and anti-glycated activities	805:858	Especially SPS-3 displayed the strongest antioxidant and anti-glycated activities.					
30732783	5	17	from	production	970:979	arg1	group					1055:1059	the protein thiol group	1037:1059	the protein thiol group	1037:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	18	theme	thiol	1049:1053	arg1	group					1055:1059	the protein thiol group	1037:1059	the protein thiol group	1037:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	0	19	theme	fractions	35:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.	0:139	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	1	20	theme	water-eluted	280:291	arg1	polysaccharide					293:306	water-eluted polysaccharide	280:306	water-eluted polysaccharide (WPS)	280:312	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	1	20	theme	water-eluted	280:291	arg1	WPS					309:311	WPS	309:311	WPS	309:311	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	2	21	theme	-rich	524:528	arg1	pectin					530:535	rhamnogalacturonan (RG)-rich pectin	501:535	rhamnogalacturonan (RG)-rich pectin	501:535	The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides.					
30732783	0	22	theme	polysaccharide	20:33	arg1	fractions					35:43	polysaccharide fractions	20:43	polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities	20:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	23	theme	effects	1165:1171	arg1	mechanisms					1138:1147	its mechanisms	1134:1147	its mechanisms of antiglycated effects	1134:1171	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	0	24	theme	activities	129:138	arg1	assessment					80:89	assessment	80:89	assessment of their antioxidant and antiglycated activities	80:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	24	theme	activities	129:138	arg1	fruit					50:54	fruit	50:54	fruit of Actinidia arguta	50:74	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	25	theme	protein	1041:1047	arg1	group					1055:1059	the protein thiol group	1037:1059	the protein thiol group	1037:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	2	26	theme	structural	403:412	arg1	analysis					414:421	structural analysis	403:421	structural analysis	403:421	The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides.					
30732783	3	27	theme	protein	718:724	arg1	glycation					726:734	protein glycation	718:734	protein glycation	718:734	All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS.					
30732783	0	28	from	assessment	80:89	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.	0:139	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	28	from	assessment	80:89	arg1	fractions					35:43	polysaccharide fractions	20:43	polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities	20:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	29	theme	carbonyl	992:999	arg1	group					1001:1005	protein carbonyl group	984:1005	protein carbonyl group	984:1005	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	30	from	group	1001:1005	arg1	group					1055:1059	the protein thiol group	1037:1059	the protein thiol group	1037:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	31	theme	group	1001:1005	arg1	production					970:979	the production	966:979	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	1	32	theme	Actinidia	216:224	arg1	arguta					226:231	Actinidia arguta	216:231	Actinidia arguta	216:231	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	0	33	theme	Actinidia	59:67	arg1	arguta					69:74	Actinidia arguta	59:74	Actinidia arguta	59:74	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	34	from	group	1055:1059	arg1	production					970:979	the production	966:979	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	2	35	theme	-rich	477:481	arg1	pectin					483:488	homogalacturonan (HG)-rich pectin	456:488	homogalacturonan (HG)-rich pectin	456:488	The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides.					
30732783	5	36	theme	scavenging	1073:1082	arg1	capacity					1084:1091	the scavenging capacity	1069:1091	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	3	37	theme	iron	669:672	arg1	ion					674:676	iron ion	669:676	iron ion	669:676	All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS.					
30732783	5	38	theme	protective	1015:1024	arg1	effects					1026:1032	the protective effects	1011:1032	the protective effects on the protein thiol group	1011:1059	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	39	theme	AAPs	899:902	arg1	effect					889:894	the inhibitory effect	874:894	the inhibitory effect of AAPs on AGEs formation	874:920	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	1	40	theme	salt-eluted	315:325	arg1	SPS					343:345	SPS	343:345	SPS	343:345	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	1	40	theme	salt-eluted	315:325	arg1	polysaccharide					327:340	salt-eluted polysaccharide (SPS)-1	315:348	salt-eluted polysaccharide (SPS)-1	315:348	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	5	41	theme	effects	1026:1032	arg1	production					970:979	the production	966:979	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	42	from	effect	889:894	arg1	formation					912:920	AGEs formation	907:920	AGEs formation	907:920	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	1	43	theme	arguta	226:231	arg1	fruits					206:211	the fruits	202:211	the fruits of Actinidia arguta	202:231	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	3	44	theme	polysaccharides	596:610	arg1	kinds					587:591	All four kinds	578:591	All four kinds of polysaccharides	578:610	All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS.					
30732783	1	45	theme	novel	143:147	arg1	polysaccharides					159:173	A novel cell-wall polysaccharides	141:173	A novel cell-wall polysaccharides (AAPs)	141:180	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	1	45	theme	novel	143:147	arg1	AAPs					176:179	AAPs	176:179	AAPs	176:179	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	5	46	theme	antioxidant	1200:1210	arg1	activities					1212:1221	their antioxidant activities	1194:1221	their antioxidant activities	1194:1221	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	3	47	theme	free	646:649	arg1	radicals					651:658	free radicals	646:658	free radicals	646:658	All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS.					
30732783	1	48	theme	cell-wall	149:157	arg1	polysaccharides					159:173	A novel cell-wall polysaccharides	141:173	A novel cell-wall polysaccharides (AAPs)	141:180	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	1	48	theme	cell-wall	149:157	arg1	AAPs					176:179	AAPs	176:179	AAPs	176:179	A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3.					
30732783	0	49	theme	arguta	69:74	arg1	assessment					80:89	assessment	80:89	assessment of their antioxidant and antiglycated activities	80:138	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	0	49	theme	arguta	69:74	arg1	fruit					50:54	fruit	50:54	fruit of Actinidia arguta	50:74	Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities.					
30732783	5	50	theme	protein	984:990	arg1	group					1001:1005	protein carbonyl group	984:1005	protein carbonyl group	984:1005	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	51	from	activity	954:961	arg1	capacity					1084:1091	the scavenging capacity	1069:1091	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30732783	5	51	from	activity	954:961	arg1	production					970:979	the production	966:979	the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds	966:1115	In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.					
30833330	11	0	theme	branched	1517:1524	arg1	glycans					1526:1532	branched glycans	1517:1532	branched glycans	1517:1532	These results suggest that RDE (II) specifically modulates branched glycans on IgE, thereby interfering with its ability to induce allergic responses.					
30833330	10	1	theme	IgE	1347:1349	arg1	binding					1336:1342	the binding	1332:1342	the binding of IgE to Lycopersicon esculentum lectin, which recognizes poly-N-acetylglucosamine and poly-N-acetyllactosamine	1332:1455	We observed that RDE (II) treatment significantly reduced the binding of IgE to Lycopersicon esculentum lectin, which recognizes poly-N-acetylglucosamine and poly-N-acetyllactosamine.					
30833330	1	2	theme	Fc	222:223	arg1	receptor					225:232	the Fc receptor	218:232	the Fc receptor	218:232	IgE plays a key role in allergies by binding to allergens and then sensitizing mast cells through the Fc receptor, resulting in the secretion of proinflammatory mediators.					
30833330	7	3	theme	mouse	956:960	arg1	ears					962:965	mouse ears	956:965	mouse ears	956:965	We also noted that RDE (II)-treated IgE could not induce passive cutaneous anaphylaxis in mouse ears.					
30833330	10	4	theme	RDE	1291:1293	arg1	treatment					1300:1308	RDE (II) treatment	1291:1308	RDE (II) treatment	1291:1308	We observed that RDE (II) treatment significantly reduced the binding of IgE to Lycopersicon esculentum lectin, which recognizes poly-N-acetylglucosamine and poly-N-acetyllactosamine.					
30833330	8	5	theme	allergic	1072:1079	arg1	responses					1081:1089	allergic responses	1072:1089	allergic responses	1072:1089	Taken together, we concluded that RDE (II) modulates the IgE structure and renders it unable to mediate allergic responses.					
30833330	2	6	theme	major	312:316	arg1	target					318:323	a major target	310:323	a major target for managing allergies	310:346	Therefore, IgE is a major target for managing allergies.					
30833330	2	6	theme	major	312:316	arg1	IgE					303:305	IgE	303:305	IgE	303:305	Therefore, IgE is a major target for managing allergies.					
30833330	3	7	from	oligomannose	385:396	arg1	IgE					401:403	IgE	401:403	IgE	401:403	Previous studies have reported that oligomannose on IgE can be a potential target to inhibit allergic responses.					
30833330	5	8	from	fluid	631:635	arg1	RDE					592:594	RDE	592:594	RDE	592:594	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	8	from	fluid	631:635	arg1	II					598:599	II	598:599	II	598:599	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	8	from	fluid	631:635	arg1	enzyme					584:589	the commercial receptor-destroying enzyme	549:589	the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid	549:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	0	9	from	cholerae	45:52	arg1	RDE					28:30	RDE	28:30	RDE	28:30	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	0	9	from	cholerae	45:52	arg1	enzyme					20:25	Receptor-destroying enzyme	0:25	Receptor-destroying enzyme (RDE) from Vibrio cholerae	0:52	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	6	10	theme	mast	797:800	arg1	cells					802:806	bone marrow-derived mast cells	777:806	bone marrow-derived mast cells	777:806	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	5	11	theme	receptor-destroying	564:582	arg1	RDE					592:594	RDE	592:594	RDE	592:594	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	11	theme	receptor-destroying	564:582	arg1	II					598:599	II	598:599	II	598:599	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	11	theme	receptor-destroying	564:582	arg1	enzyme					584:589	the commercial receptor-destroying enzyme	549:589	the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid	549:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	12	theme	commercial	553:562	arg1	RDE					592:594	RDE	592:594	RDE	592:594	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	12	theme	commercial	553:562	arg1	II					598:599	II	598:599	II	598:599	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	5	12	theme	commercial	553:562	arg1	enzyme					584:589	the commercial receptor-destroying enzyme	549:589	the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid	549:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	12	13	from	activity	1686:1693	arg1	allergies					1698:1706	allergies	1698:1706	allergies	1698:1706	Our findings may provide a basis for the development of drugs to inhibit IgE activity in allergies.					
30833330	8	14	theme	IgE	1025:1027	arg1	structure					1029:1037	the IgE structure	1021:1037	the IgE structure	1021:1037	Taken together, we concluded that RDE (II) modulates the IgE structure and renders it unable to mediate allergic responses.					
30833330	6	15	theme	marrow-derived	782:795	arg1	cells					802:806	bone marrow-derived mast cells	777:806	bone marrow-derived mast cells	777:806	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	3	16	theme	Previous	349:356	arg1	studies					358:364	Previous studies	349:364	Previous studies	349:364	Previous studies have reported that oligomannose on IgE can be a potential target to inhibit allergic responses.					
30833330	9	17	theme	microarray	1184:1193	arg1	analysis					1195:1202	lectin microarray analysis	1177:1202	lectin microarray analysis	1177:1202	To reveal the mechanism by which RDE (II) interferes with IgE activity, we performed lectin microarray analysis to unravel the relationship between IgE modulation and glycosylation.					
30833330	7	18	theme	passive	923:929	arg1	anaphylaxis					941:951	passive cutaneous anaphylaxis	923:951	passive cutaneous anaphylaxis	923:951	We also noted that RDE (II)-treated IgE could not induce passive cutaneous anaphylaxis in mouse ears.					
30833330	0	19	theme	Receptor-destroying	0:18	arg1	RDE					28:30	RDE	28:30	RDE	28:30	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	0	19	theme	Receptor-destroying	0:18	arg1	enzyme					20:25	Receptor-destroying enzyme	0:25	Receptor-destroying enzyme (RDE) from Vibrio cholerae	0:52	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	6	20	link	marrow-derived	782:795	arg1	cells					802:806	bone marrow-derived mast cells	777:806	bone marrow-derived mast cells	777:806	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	9	21	theme	lectin	1177:1182	arg1	analysis					1195:1202	lectin microarray analysis	1177:1202	lectin microarray analysis	1177:1202	To reveal the mechanism by which RDE (II) interferes with IgE activity, we performed lectin microarray analysis to unravel the relationship between IgE modulation and glycosylation.					
30833330	0	22	theme	anaphylaxis	107:117	arg1	initiation					93:102	the initiation	89:102	the initiation of anaphylaxis	89:117	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	6	23	theme	cytokines	855:863	arg1	release					830:836	reduced release	822:836	reduced release of histamine and cytokines	822:863	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	7	24	theme	cutaneous	931:939	arg1	anaphylaxis					941:951	passive cutaneous anaphylaxis	923:951	passive cutaneous anaphylaxis	923:951	We also noted that RDE (II)-treated IgE could not induce passive cutaneous anaphylaxis in mouse ears.					
30833330	1	25	theme	proinflammatory	265:279	arg1	mediators					281:289	proinflammatory mediators	265:289	proinflammatory mediators	265:289	IgE plays a key role in allergies by binding to allergens and then sensitizing mast cells through the Fc receptor, resulting in the secretion of proinflammatory mediators.					
30833330	6	26	theme	binding	761:767	arg1	site					769:772	its binding site	757:772	its binding site	757:772	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	7	27	theme	-treated	893:900	arg1	IgE					902:904	RDE (II)-treated IgE	885:904	RDE (II)-treated IgE	885:904	We also noted that RDE (II)-treated IgE could not induce passive cutaneous anaphylaxis in mouse ears.					
30833330	3	28	theme	potential	414:422	arg1	target					424:429	a potential target	412:429	a potential target to inhibit allergic responses	412:459	Previous studies have reported that oligomannose on IgE can be a potential target to inhibit allergic responses.					
30833330	3	28	theme	potential	414:422	arg1	oligomannose					385:396	oligomannose	385:396	oligomannose on IgE	385:403	Previous studies have reported that oligomannose on IgE can be a potential target to inhibit allergic responses.					
30833330	5	29	theme	anaphylaxis	709:719	arg1	initiation					695:704	the initiation	691:704	the initiation of anaphylaxis	691:719	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	1	30	theme	mediators	281:289	arg1	secretion					252:260	the secretion	248:260	the secretion of proinflammatory mediators	248:289	IgE plays a key role in allergies by binding to allergens and then sensitizing mast cells through the Fc receptor, resulting in the secretion of proinflammatory mediators.					
30833330	10	31	theme	esculentum	1367:1376	arg1	lectin					1378:1383	Lycopersicon esculentum lectin	1354:1383	Lycopersicon esculentum lectin	1354:1383	We observed that RDE (II) treatment significantly reduced the binding of IgE to Lycopersicon esculentum lectin, which recognizes poly-N-acetylglucosamine and poly-N-acetyllactosamine.					
30833330	10	32	theme	Lycopersicon	1354:1365	arg1	lectin					1378:1383	Lycopersicon esculentum lectin	1354:1383	Lycopersicon esculentum lectin	1354:1383	We observed that RDE (II) treatment significantly reduced the binding of IgE to Lycopersicon esculentum lectin, which recognizes poly-N-acetylglucosamine and poly-N-acetyllactosamine.					
30833330	0	33	theme	Vibrio	38:43	arg1	cholerae					45:52	Vibrio cholerae	38:52	Vibrio cholerae	38:52	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	5	34	theme	Vibrio	607:612	arg1	fluid					631:635	Vibrio cholerae culture fluid	607:635	Vibrio cholerae culture fluid	607:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	11	35	theme	allergic	1589:1596	arg1	responses					1598:1606	allergic responses	1589:1606	allergic responses	1589:1606	These results suggest that RDE (II) specifically modulates branched glycans on IgE, thereby interfering with its ability to induce allergic responses.					
30833330	4	36	theme	IgE	497:499	arg1	activity					501:508	IgE activity	497:508	IgE activity	497:508	However, enzymes that can modulate IgE activity are not yet known.					
30833330	5	37	theme	cholerae	614:621	arg1	fluid					631:635	Vibrio cholerae culture fluid	607:635	Vibrio cholerae culture fluid	607:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	12	38	theme	IgE	1682:1684	arg1	activity					1686:1693	IgE activity	1682:1693	IgE activity in allergies	1682:1706	Our findings may provide a basis for the development of drugs to inhibit IgE activity in allergies.					
30833330	9	39	theme	IgE	1150:1152	arg1	activity					1154:1161	IgE activity	1150:1161	IgE activity	1150:1161	To reveal the mechanism by which RDE (II) interferes with IgE activity, we performed lectin microarray analysis to unravel the relationship between IgE modulation and glycosylation.					
30833330	1	40	theme	mast	199:202	arg1	cells					204:208	mast cells	199:208	mast cells	199:208	IgE plays a key role in allergies by binding to allergens and then sensitizing mast cells through the Fc receptor, resulting in the secretion of proinflammatory mediators.					
30833330	5	41	theme	culture	623:629	arg1	fluid					631:635	Vibrio cholerae culture fluid	607:635	Vibrio cholerae culture fluid	607:635	Here, we found that the commercial receptor-destroying enzyme (RDE) (II) from Vibrio cholerae culture fluid specifically modulates IgE, but not IgG, and prevents the initiation of anaphylaxis.					
30833330	3	42	theme	allergic	442:449	arg1	responses					451:459	allergic responses	442:459	allergic responses	442:459	Previous studies have reported that oligomannose on IgE can be a potential target to inhibit allergic responses.					
30833330	0	43	theme	IgE	64:66	arg1	activity					68:75	IgE activity	64:75	IgE activity	64:75	Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis.					
30833330	6	44	theme	-treated	730:737	arg1	IgE					739:741	RDE (II)-treated IgE	722:741	RDE (II)-treated IgE	722:741	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	9	45	theme	IgE	1240:1242	arg1	modulation					1244:1253	IgE modulation	1240:1253	IgE modulation	1240:1253	To reveal the mechanism by which RDE (II) interferes with IgE activity, we performed lectin microarray analysis to unravel the relationship between IgE modulation and glycosylation.					
30833330	6	46	from	access	750:755	arg1	cells					802:806	bone marrow-derived mast cells	777:806	bone marrow-derived mast cells	777:806	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	6	47	theme	reduced	822:828	arg1	release					830:836	reduced release	822:836	reduced release of histamine and cytokines	822:863	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30833330	12	48	theme	drugs	1665:1669	arg1	development					1650:1660	the development	1646:1660	the development of drugs to inhibit IgE activity in allergies	1646:1706	Our findings may provide a basis for the development of drugs to inhibit IgE activity in allergies.					
30833330	1	49	theme	key	132:134	arg1	role					136:139	a key role	130:139	a key role	130:139	IgE plays a key role in allergies by binding to allergens and then sensitizing mast cells through the Fc receptor, resulting in the secretion of proinflammatory mediators.					
30833330	6	50	theme	histamine	841:849	arg1	release					830:836	reduced release	822:836	reduced release of histamine and cytokines	822:863	RDE (II)-treated IgE cannot access its binding site on bone marrow-derived mast cells, resulting in reduced release of histamine and cytokines.					
30392564	5	0	theme	additional	735:744	arg1	compounds					746:754	additional compounds	735:754	additional compounds mimicking hMOS in structure and function	735:795	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	4	1	theme	growth	660:665	arg1	effects					679:685	growth stimulatory effects	660:685	growth stimulatory effects on various probiotic bacteria	660:715	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30392564	7	2	with	end	1266:1268	arg1	moiety					1292:1297	an (α1→2) linked moiety	1275:1297	an (α1→2) linked moiety	1275:1297	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	3	3	dep	compounds	550:558	arg1	degree					561:566	degree	561:566	degree of polymerization 3-4	561:588	Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages.					
30392564	6	4	used	used	972:975	arg2	Gtf180-ΔN					895:903	Gtf180-ΔN	895:903	Gtf180-ΔN	895:903	Both Gtf180-ΔN and GtfA-ΔN were unable to use 3'-galactosyl-lactose (β3'-GL), but used sucrose to add a single glucose moiety to 4'-galactosyl-lactose (β4'-GL) and 6'-galactosyl-lactose (β6'-GL).					
30392564	8	5	used	used	1349:1352	arg2	Glucansucrases					1300:1313	Glucansucrases Gtf180-ΔN and GtfA-ΔN	1300:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN	1300:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	5	used	used	1349:1352	arg2	GtfA-ΔN					1329:1335	GtfA-ΔN	1329:1335	GtfA-ΔN	1329:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	5	used	used	1349:1352	arg2	Gtf180-ΔN					1315:1323	Gtf180-ΔN	1315:1323	Gtf180-ΔN	1315:1323	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	0	6	theme	Gtf180	110:115	arg1	enzymes					131:137	Gtf180 glucansucrase enzymes	110:137	Gtf180 glucansucrase enzymes	110:137	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	5	7	from	hMOS	766:769	arg1	function					788:795	function	788:795	function	788:795	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	5	7	from	hMOS	766:769	arg1	structure					774:782	structure	774:782	structure	774:782	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	2	8	dep	α1→6	390:393	arg1	linkages					432:439	glucosidic linkages	421:439	glucosidic linkages	421:439	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	7	9	link	-linked	1151:1157	arg1	moiety					1159:1164	an (α1→2)-linked moiety	1142:1164	an (α1→2)-linked moiety	1142:1164	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	1	10	used	used	177:180	arg2	GOS					168:170	GOS	168:170	GOS	168:170	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	1	10	used	used	177:180	arg2	β-Galacto-oligosaccharides					140:165	β-Galacto-oligosaccharides	140:165	β-Galacto-oligosaccharides (GOS)	140:171	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	7	11	theme	linked	1209:1214	arg1	moiety					1216:1221	an (α1→4) linked moiety	1199:1221	an (α1→4) linked moiety	1199:1221	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	8	12	theme	glucosidic	1437:1446	arg1	linkages					1448:1455	glucosidic linkages	1437:1455	glucosidic linkages	1437:1455	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	7	13	dep	moiety	1292:1297	arg1	α1→2					1279:1282	α1→2	1279:1282	α1→2	1279:1282	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	6	14	theme	glucose	1001:1007	arg1	moiety					1009:1014	a single glucose moiety	992:1014	a single glucose moiety	992:1014	Both Gtf180-ΔN and GtfA-ΔN were unable to use 3'-galactosyl-lactose (β3'-GL), but used sucrose to add a single glucose moiety to 4'-galactosyl-lactose (β4'-GL) and 6'-galactosyl-lactose (β6'-GL).					
30392564	5	15	theme	available	846:854	arg1	compounds					879:887	3 commercially available galactosyl-lactose GOS compounds	831:887	3 commercially available galactosyl-lactose GOS compounds	831:887	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	7	16	theme	reducing	1257:1264	arg1	end					1266:1268	its reducing end	1253:1268	its reducing end with an (α1→2) linked moiety	1253:1297	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	0	17	theme	glucansucrase	117:129	arg1	enzymes					131:137	Gtf180 glucansucrase enzymes	110:137	Gtf180 glucansucrase enzymes	110:137	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	7	18	theme	glucosyl	1123:1130	arg1	unit					1132:1135	its reducing glucosyl unit	1110:1135	its reducing glucosyl unit with an (α1→2)-linked moiety	1110:1164	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	6	19	theme	single	994:999	arg1	moiety					1009:1014	a single glucose moiety	992:1014	a single glucose moiety	992:1014	Both Gtf180-ΔN and GtfA-ΔN were unable to use 3'-galactosyl-lactose (β3'-GL), but used sucrose to add a single glucose moiety to 4'-galactosyl-lactose (β4'-GL) and 6'-galactosyl-lactose (β6'-GL).					
30392564	1	20	theme	infant	198:203	arg1	nutrition					205:213	infant nutrition	198:213	infant nutrition	198:213	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	2	21	theme	glucosidic	421:430	arg1	linkages					432:439	glucosidic linkages	421:439	glucosidic linkages	421:439	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	8	22	theme	bioactive	1484:1492	arg1	properties					1506:1515	valuable bioactive (prebiotic) properties	1475:1515	valuable bioactive (prebiotic) properties	1475:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	7	23	theme	non-reducing	1177:1188	arg1	end					1190:1192	its non-reducing end	1173:1192	its non-reducing end with an (α1→4) linked moiety	1173:1221	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	3	24	theme	compounds	550:558	arg1	minimum					537:543	a minimum	535:543	a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages	535:629	Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages.					
30392564	2	25	dep	Glucansucrases	283:296	arg1	GtfA-ΔN					312:318	GtfA-ΔN	312:318	GtfA-ΔN	312:318	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	25	dep	Glucansucrases	283:296	arg1	Gtf180-ΔN					298:306	Gtf180-ΔN	298:306	Gtf180-ΔN	298:306	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	25	dep	Glucansucrases	283:296	arg1	Glucansucrases					283:296	Glucansucrases Gtf180-ΔN and GtfA-ΔN	283:318	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains	283:351	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	0	26	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of glucosylated GOS derivatives	0:58	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	8	27	theme	valuable	1475:1482	arg1	properties					1506:1515	valuable bioactive (prebiotic) properties	1475:1515	valuable bioactive (prebiotic) properties	1475:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	2	28	theme	strains	345:351	arg1	GtfA-ΔN					312:318	GtfA-ΔN	312:318	GtfA-ΔN	312:318	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	28	theme	strains	345:351	arg1	Gtf180-ΔN					298:306	Gtf180-ΔN	298:306	Gtf180-ΔN	298:306	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	28	theme	strains	345:351	arg1	Glucansucrases					283:296	Glucansucrases Gtf180-ΔN and GtfA-ΔN	283:318	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains	283:351	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	5	29	theme	galactosyl-lactose	856:873	arg1	compounds					879:887	3 commercially available galactosyl-lactose GOS compounds	831:887	3 commercially available galactosyl-lactose GOS compounds	831:887	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	0	30	theme	glucosylated	31:42	arg1	derivatives					48:58	glucosylated GOS derivatives	31:58	glucosylated GOS derivatives	31:58	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	8	31	theme	galactosyl-lactose-derived	1365:1390	arg1	oligosaccharides					1392:1407	galactosyl-lactose-derived oligosaccharides	1365:1407	galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties	1365:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	0	32	dep	Lactobacillus	79:91	arg1	reuteri					93:99	reuteri	93:99	reuteri	93:99	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	4	33	theme	probiotic	698:706	arg1	bacteria					708:715	various probiotic bacteria	690:715	various probiotic bacteria	690:715	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30392564	7	34	dep	moiety	1216:1221	arg1	α1→4					1203:1206	α1→4	1203:1206	α1→4	1203:1206	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	2	35	theme	Lactobacillus	323:335	arg1	strains					345:351	Lactobacillus reuteri strains	323:351	Lactobacillus reuteri strains	323:351	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	0	36	theme	derivatives	48:58	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of glucosylated GOS derivatives	0:58	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	4	37	theme	stimulatory	667:677	arg1	effects					679:685	growth stimulatory effects	660:685	growth stimulatory effects on various probiotic bacteria	660:715	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30392564	3	38	theme	polymerization	571:584	arg1	degree					561:566	degree	561:566	degree of polymerization 3-4	561:588	Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages.					
30392564	8	39	with	linkages	1448:1455	arg1	properties					1506:1515	valuable bioactive (prebiotic) properties	1475:1515	valuable bioactive (prebiotic) properties	1475:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	0	40	theme	GOS	44:46	arg1	derivatives					48:58	glucosylated GOS derivatives	31:58	glucosylated GOS derivatives	31:58	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	7	41	link	linked	1285:1290	arg1	moiety					1292:1297	an (α1→2) linked moiety	1275:1297	an (α1→2) linked moiety	1275:1297	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	3	42	theme	3-4	586:588	arg1	polymerization					571:584	polymerization 3-4	571:588	polymerization 3-4	571:588	Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages.					
30392564	7	43	theme	-linked	1151:1157	arg1	moiety					1159:1164	an (α1→2)-linked moiety	1142:1164	an (α1→2)-linked moiety	1142:1164	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	8	44	dep	Glucansucrases	1300:1313	arg1	Glucansucrases					1300:1313	Glucansucrases Gtf180-ΔN and GtfA-ΔN	1300:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN	1300:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	44	dep	Glucansucrases	1300:1313	arg1	GtfA-ΔN					1329:1335	GtfA-ΔN	1329:1335	GtfA-ΔN	1329:1335	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	44	dep	Glucansucrases	1300:1313	arg1	Gtf180-ΔN					1315:1323	Gtf180-ΔN	1315:1323	Gtf180-ΔN	1315:1323	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	45	theme	prebiotic	1495:1503	arg1	properties					1506:1515	valuable bioactive (prebiotic) properties	1475:1515	valuable bioactive (prebiotic) properties	1475:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	2	46	with	α-glucans	374:382	arg1	α1→4					408:411	(α1→4)/	407:413	(α1→4)/(α1→6)	407:419	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	46	with	α-glucans	374:382	arg1	α1→3					397:400	α1→3	397:400	α1→3	397:400	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	46	with	α-glucans	374:382	arg1	α1→6					415:418	α1→6	415:418	α1→6	415:418	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	2	46	with	α-glucans	374:382	arg1	α1→6					390:393	(α1→6)/	389:395	(α1→6)/(α1→3)	389:401	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	7	47	theme	reducing	1114:1121	arg1	unit					1132:1135	its reducing glucosyl unit	1110:1135	its reducing glucosyl unit with an (α1→2)-linked moiety	1110:1164	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	7	48	link	linked	1209:1214	arg1	moiety					1216:1221	an (α1→4) linked moiety	1199:1221	an (α1→4) linked moiety	1199:1221	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	7	49	theme	linked	1285:1290	arg1	moiety					1292:1297	an (α1→2) linked moiety	1275:1297	an (α1→2) linked moiety	1275:1297	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	2	50	dep	Lactobacillus	323:335	arg1	reuteri					337:343	reuteri	337:343	reuteri	337:343	Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively.					
30392564	3	51	with	compounds	550:558	arg1	linkages					622:629	(α1→2/3/4) linkages	611:629	(α1→2/3/4) linkages	611:629	Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages.					
30392564	4	52	theme	various	690:696	arg1	bacteria					708:715	various probiotic bacteria	690:715	various probiotic bacteria	690:715	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30392564	8	53	with	α1→2	1421:1424	arg1	properties					1506:1515	valuable bioactive (prebiotic) properties	1475:1515	valuable bioactive (prebiotic) properties	1475:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	1	54	theme	human	247:251	arg1	oligosaccharides					258:273	human milk oligosaccharides	247:273	human milk oligosaccharides (hMOS)	247:280	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	1	54	theme	human	247:251	arg1	hMOS					276:279	hMOS	276:279	hMOS	276:279	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	7	55	with	unit	1132:1135	arg1	moiety					1159:1164	an (α1→2)-linked moiety	1142:1164	an (α1→2)-linked moiety	1142:1164	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	5	56	theme	compounds	879:887	arg1	glucosylation					814:826	glucosylation	814:826	glucosylation of 3 commercially available galactosyl-lactose GOS compounds	814:887	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	4	57	from	effects	679:685	arg1	bacteria					708:715	various probiotic bacteria	690:715	various probiotic bacteria	690:715	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30392564	1	58	theme	milk	253:256	arg1	oligosaccharides					258:273	human milk oligosaccharides	247:273	human milk oligosaccharides (hMOS)	247:280	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	1	58	theme	milk	253:256	arg1	hMOS					276:279	hMOS	276:279	hMOS	276:279	β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS).					
30392564	0	59	theme	Lactobacillus	79:91	arg1	GtfA					101:104	the Lactobacillus reuteri GtfA	75:104	the Lactobacillus reuteri GtfA	75:104	Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes.					
30392564	8	60	contain	containing	1409:1418	arg2	linkages					1448:1455	glucosidic linkages	1437:1455	glucosidic linkages	1437:1455	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	60	contain	containing	1409:1418	arg1	oligosaccharides					1392:1407	galactosyl-lactose-derived oligosaccharides	1365:1407	galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties	1365:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	60	contain	containing	1409:1418	arg2	α1→2					1421:1424	(α1→2)	1420:1425	(α1→2)	1420:1425	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	5	61	theme	GOS	875:877	arg1	compounds					879:887	3 commercially available galactosyl-lactose GOS compounds	831:887	3 commercially available galactosyl-lactose GOS compounds	831:887	Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds.					
30392564	8	62	link	galactosyl-lactose-derived	1365:1390	arg1	oligosaccharides					1392:1407	galactosyl-lactose-derived oligosaccharides	1365:1407	galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties	1365:1515	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	8	63	dep	linkages	1448:1455	arg1	α→4					1432:1434	α→4	1432:1434	α→4	1432:1434	Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.					
30392564	7	64	with	end	1190:1192	arg1	moiety					1216:1221	an (α1→4) linked moiety	1199:1221	an (α1→4) linked moiety	1199:1221	β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety.					
30392564	4	65	theme	GL34	637:640	arg1	mixture					642:648	This GL34 mixture	632:648	This GL34 mixture	632:648	This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria.					
30742925	0	0	theme	antioxidant	99:109	arg1	activities					129:138	their antioxidant and hypolipidemic activities	93:138	their antioxidant and hypolipidemic activities	93:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	2	1	theme	due	445:447	arg1	%					443:443	23.1%	439:443	23.1% due to pectin structure	439:467	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	2	2	theme	neutral	388:394	arg1	sugars					396:401	neutral sugars	388:401	neutral sugars (69.1%) but also uronic acids	388:431	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	2	2	theme	neutral	388:394	arg1	%					408:408	69.1%	404:408	69.1%	404:408	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	0	3	from	characterization	11:26	arg1	fruits					82:87	Nitraria retusa fruits	66:87	Nitraria retusa fruits	66:87	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	0	3	from	characterization	11:26	arg1	activities					129:138	their antioxidant and hypolipidemic activities	93:138	their antioxidant and hypolipidemic activities	93:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	4	4	theme	scavenging	738:747	arg1	activities					749:758	scavenging activities	738:758	scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml)	738:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	4	theme	scavenging	738:747	arg1	capacity					708:715	effective total antioxidant capacity	680:715	effective total antioxidant capacity (IC50 = 7.82 mg/ml)	680:735	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	1	5	from	activities	192:201	arg1	Nitraria					227:234	Nitraria	227:234	Nitraria	227:234	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	2	6	theme	β-	359:360	arg1	-glucan					368:374	a β-(1 → 3)-glucan	357:374	a β-(1 → 3)-glucan	357:374	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	0	7	theme	hypolipidemic	115:127	arg1	activities					129:138	their antioxidant and hypolipidemic activities	93:138	their antioxidant and hypolipidemic activities	93:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	4	8	theme	DPPH	763:766	arg1	IC50 = 0.87 mg/ml					777:793	IC50 = 0.87 mg/ml	777:793	IC50 = 0.87 mg/ml	777:793	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	8	theme	DPPH	763:766	arg1	radical					768:774	DPPH radical	763:774	DPPH radical (IC50 = 0.87 mg/ml)	763:794	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	2	9	theme	molecular	311:319	arg1	weight					321:326	a molecular weight	309:326	a molecular weight of 66.5 kDa	309:338	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	4	10	theme	antioxidant	645:655	arg1	assays					657:662	the antioxidant assays	641:662	the antioxidant assays	641:662	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	1	11	theme	polysaccharides	206:220	arg1	antioxidant					156:166	antioxidant	156:166	antioxidant	156:166	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	11	theme	polysaccharides	206:220	arg1	activities					192:201	anti-hyperlipidemic activities	172:201	anti-hyperlipidemic activities	172:201	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	11	theme	polysaccharides	206:220	arg1	structure					145:153	structure	145:153	structure	145:153	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	12	dep	structure	145:153	arg1	The					141:143	The	141:143	The	141:143	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	2	13	theme	uronic	420:425	arg1	acids					427:431	uronic acids	420:431	neutral sugars (69.1%) but also uronic acids	388:431	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	3	14	theme	monosaccharide	474:487	arg1	composition					489:499	The monosaccharide composition	470:499	The monosaccharide composition	470:499	The monosaccharide composition highlighted a polymer composed of glucose (41.4%), galacturonic acid (30.5%), galactose (12.6%), arabinose (11.8%) and rhamnose (3.70%).					
30742925	1	15	from	antioxidant	156:166	arg1	Nitraria					227:234	Nitraria	227:234	Nitraria	227:234	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	5	16	from	effects	875:881	arg1	H2O2					886:889	H2O2	886:889	H2O2 induced hemolysis (IC50 = 66.2 μg/ml)	886:927	In addition, NRFP proved protective effects on H2O2 induced hemolysis (IC50 = 66.2 μg/ml).					
30742925	0	17	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.	0:139	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	2	18	theme	66.5 kDa	331:338	arg1	weight					321:326	a molecular weight	309:326	a molecular weight of 66.5 kDa	309:338	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	0	19	theme	water-soluble	31:43	arg1	polysaccharides					45:59	water-soluble polysaccharides	31:59	water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities	31:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	3	20	theme	galacturonic	552:563	arg1	acid					565:568	galacturonic acid	552:568	galacturonic acid (30.5%)	552:576	The monosaccharide composition highlighted a polymer composed of glucose (41.4%), galacturonic acid (30.5%), galactose (12.6%), arabinose (11.8%) and rhamnose (3.70%).					
30742925	3	20	theme	galacturonic	552:563	arg1	%					575:575	30.5%	571:575	30.5%	571:575	The monosaccharide composition highlighted a polymer composed of glucose (41.4%), galacturonic acid (30.5%), galactose (12.6%), arabinose (11.8%) and rhamnose (3.70%).					
30742925	4	21	theme	hydrogen	800:807	arg1	IC50 = 2.03 mg/ml					819:835	IC50 = 2.03 mg/ml	819:835	IC50 = 2.03 mg/ml	819:835	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	21	theme	hydrogen	800:807	arg1	peroxide					809:816	hydrogen peroxide	800:816	hydrogen peroxide (IC50 = 2.03 mg/ml)	800:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	1	22	from	structure	145:153	arg1	Nitraria					227:234	Nitraria	227:234	Nitraria	227:234	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	6	23	theme	Triton	1035:1040	arg1	X-100					1042:1046	Triton X-100	1035:1046	Triton X-100	1035:1046	In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.					
30742925	4	24	theme	total	690:694	arg1	activities					749:758	scavenging activities	738:758	scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml)	738:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	24	theme	total	690:694	arg1	IC50 = 7.82 mg/ml					718:734	IC50 = 7.82 mg/ml	718:734	IC50 = 7.82 mg/ml	718:734	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	24	theme	total	690:694	arg1	capacity					708:715	effective total antioxidant capacity	680:715	effective total antioxidant capacity (IC50 = 7.82 mg/ml)	680:735	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	6	25	theme	In	930:931	arg1	NRFP					938:941	In vivo NRFP	930:941	In vivo NRFP	930:941	In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.					
30742925	1	26	dep	Nitraria	227:234	arg1	retusa					236:241	Nitraria retusa fruits (named as NRFP)	227:264	Nitraria retusa fruits (named as NRFP)	227:264	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	26	dep	Nitraria	227:234	arg1	fruits					243:248	fruits	243:248	Nitraria retusa fruits (named as NRFP)	227:264	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	0	27	theme	polysaccharides	45:59	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.	0:139	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	2	28	contain	containing	377:386	arg2	%					408:408	69.1%	404:408	69.1%	404:408	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	2	28	contain	containing	377:386	arg1	-glucan					368:374	a β-(1 → 3)-glucan	357:374	a β-(1 → 3)-glucan	357:374	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	2	28	contain	containing	377:386	arg2	acids					427:431	uronic acids	420:431	neutral sugars (69.1%) but also uronic acids	388:431	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	2	28	contain	containing	377:386	arg2	sugars					396:401	neutral sugars	388:401	neutral sugars (69.1%) but also uronic acids	388:431	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	0	29	dep	Nitraria	66:73	arg1	retusa					75:80	retusa	75:80	retusa	75:80	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	0	30	from	fruits	82:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.	0:139	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	0	30	from	fruits	82:87	arg1	polysaccharides					45:59	water-soluble polysaccharides	31:59	water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities	31:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	4	31	from	activities	749:758	arg1	IC50 = 0.87 mg/ml					777:793	IC50 = 0.87 mg/ml	777:793	IC50 = 0.87 mg/ml	777:793	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	31	from	activities	749:758	arg1	radical					768:774	DPPH radical	763:774	DPPH radical (IC50 = 0.87 mg/ml)	763:794	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	31	from	activities	749:758	arg1	peroxide					809:816	hydrogen peroxide	800:816	hydrogen peroxide (IC50 = 2.03 mg/ml)	800:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	31	from	activities	749:758	arg1	IC50 = 2.03 mg/ml					819:835	IC50 = 2.03 mg/ml	819:835	IC50 = 2.03 mg/ml	819:835	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	0	32	theme	Nitraria	66:73	arg1	fruits					82:87	Nitraria retusa fruits	66:87	Nitraria retusa fruits	66:87	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	2	33	with	fraction	294:301	arg1	weight					321:326	a molecular weight	309:326	a molecular weight of 66.5 kDa	309:338	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	4	34	theme	antioxidant	696:706	arg1	activities					749:758	scavenging activities	738:758	scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml)	738:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	34	theme	antioxidant	696:706	arg1	IC50 = 7.82 mg/ml					718:734	IC50 = 7.82 mg/ml	718:734	IC50 = 7.82 mg/ml	718:734	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	34	theme	antioxidant	696:706	arg1	capacity					708:715	effective total antioxidant capacity	680:715	effective total antioxidant capacity (IC50 = 7.82 mg/ml)	680:735	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	0	35	from	activities	129:138	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.	0:139	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	0	35	from	activities	129:138	arg1	polysaccharides					45:59	water-soluble polysaccharides	31:59	water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities	31:138	Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.					
30742925	5	36	theme	protective	864:873	arg1	effects					875:881	protective effects	864:881	protective effects on H2O2 induced hemolysis (IC50 = 66.2 μg/ml)	864:927	In addition, NRFP proved protective effects on H2O2 induced hemolysis (IC50 = 66.2 μg/ml).					
30742925	4	37	theme	effective	680:688	arg1	activities					749:758	scavenging activities	738:758	scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml)	738:836	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	37	theme	effective	680:688	arg1	IC50 = 7.82 mg/ml					718:734	IC50 = 7.82 mg/ml	718:734	IC50 = 7.82 mg/ml	718:734	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	4	37	theme	effective	680:688	arg1	capacity					708:715	effective total antioxidant capacity	680:715	effective total antioxidant capacity (IC50 = 7.82 mg/ml)	680:735	In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC50 = 7.82 mg/ml), scavenging activities on DPPH radical (IC50 = 0.87 mg/ml) and hydrogen peroxide (IC50 = 2.03 mg/ml).					
30742925	6	38	theme	coronary	1006:1013	arg1	diseases					1015:1022	coronary diseases	1006:1022	coronary diseases	1006:1022	In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.					
30742925	6	39	dep	hyperlipidemia	955:968	arg1	the					951:953	the	951:953	the	951:953	In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.					
30742925	6	40	dep	In	930:931	arg1	vivo					933:936	vivo	933:936	vivo	933:936	In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.					
30742925	2	41	theme	NRFP	289:292	arg1	fraction					294:301	The NRFP fraction	285:301	The NRFP fraction	285:301	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	1	42	from	Nitraria	227:234	arg1	polysaccharides					206:220	polysaccharides	206:220	polysaccharides from Nitraria retusa fruits (named as NRFP)	206:264	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	42	from	Nitraria	227:234	arg1	antioxidant					156:166	antioxidant	156:166	antioxidant	156:166	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	42	from	Nitraria	227:234	arg1	activities					192:201	anti-hyperlipidemic activities	172:201	anti-hyperlipidemic activities	172:201	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	42	from	Nitraria	227:234	arg1	structure					145:153	structure	145:153	structure	145:153	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	1	43	theme	anti-hyperlipidemic	172:190	arg1	activities					192:201	anti-hyperlipidemic activities	172:201	anti-hyperlipidemic activities	172:201	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	2	44	theme	pectin	452:457	arg1	structure					459:467	pectin structure	452:467	pectin structure	452:467	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
30742925	1	45	dep	fruits	243:248	arg1	named					251:255	named	251:255	named as NRFP	251:263	The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated.					
30742925	2	46	theme	1 → 3	362:366	arg1	-glucan					368:374	a β-(1 → 3)-glucan	357:374	a β-(1 → 3)-glucan	357:374	The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure.					
31033942	0	0	theme	Encoded	96:102	arg1	Biosensors					104:113	Genetically Encoded Biosensors	84:113	Genetically Encoded Biosensors	84:113	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	4	1	gly	fucosylated	739:749	arg1	3-fucosyllactose					789:804	3-fucosyllactose	789:804	3-fucosyllactose	789:804	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	1	gly	fucosylated	739:749	arg1	2'-fucosyllactose					767:783	2'-fucosyllactose	767:783	2'-fucosyllactose	767:783	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	1	gly	fucosylated	739:749	arg1	structures					755:764	the fucosylated HMO structures	735:764	the fucosylated HMO structures	735:764	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	2	2	theme	quantification	377:390	arg1	throughput					349:358	the relatively low throughput	330:358	the relatively low throughput of detection and quantification of monosaccharide and linkages	330:421	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	4	3	theme	bacterial	641:649	arg1	biosensor					651:659	a genetically encoded bacterial biosensor	619:659	a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases	619:866	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	0	4	from	Analysis	0:7	arg1	Context					70:76	Biotechnological Context	53:76	Biotechnological Context	53:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	6	5	theme	manufacturing	1237:1249	arg1	optimization					1217:1228	the optimization	1213:1228	the optimization of HMO manufacturing	1213:1249	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	2	6	theme	synthesis	306:314	arg1	optimization					267:278	optimization	267:278	optimization of their biotechnological synthesis	267:314	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	1	7	theme	carbohydrate	163:174	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	7	theme	carbohydrate	163:174	arg1	components					176:185	complex carbohydrate components	155:185	complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health	155:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	3	8	theme	hundreds	550:557	arg1	order					541:545	the order	537:545	the order of hundreds of samples per day without automation	537:595	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	5	9	theme	signal	1005:1010	arg1	reduction					992:1000	the reduction	988:1000	the reduction of signal	988:1010	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	2	10	theme	biotechnological	289:304	arg1	synthesis					306:314	their biotechnological synthesis	283:314	their biotechnological synthesis	283:314	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	2	11	theme	linkages	414:421	arg1	quantification					377:390	quantification	377:390	quantification	377:390	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	2	11	theme	linkages	414:421	arg1	detection					363:371	detection	363:371	detection	363:371	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	4	12	theme	HMO	751:753	arg1	3-fucosyllactose					789:804	3-fucosyllactose	789:804	3-fucosyllactose	789:804	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	12	theme	HMO	751:753	arg1	2'-fucosyllactose					767:783	2'-fucosyllactose	767:783	2'-fucosyllactose	767:783	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	12	theme	HMO	751:753	arg1	structures					755:764	the fucosylated HMO structures	735:764	the fucosylated HMO structures	735:764	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	0	13	from	Trisaccharides	35:48	arg1	Context					70:76	Biotechnological Context	53:76	Biotechnological Context	53:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	3	14	theme	glycan	451:456	arg1	analysis					458:465	glycan analysis	451:465	glycan analysis	451:465	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	4	15	theme	high-throughput	669:683	arg1	detection					703:711	detection	703:711	detection	703:711	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	1	16	theme	human	190:194	arg1	milk					203:206	human breast milk	190:206	human breast milk	190:206	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	5	17	theme	different	1031:1039	arg1	strategies					1041:1050	different strategies	1031:1050	different strategies	1031:1050	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	1	18	theme	breast	196:201	arg1	milk					203:206	human breast milk	190:206	human breast milk	190:206	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	3	19	theme	Conventional	424:435	arg1	techniques					437:446	Conventional techniques	424:446	Conventional techniques of glycan analysis	424:465	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	4	20	theme	heterologous	829:840	arg1	expression					842:851	heterologous expression	829:851	heterologous expression of fucosidases	829:866	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	1	21	theme	milk	203:206	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	21	theme	milk	203:206	arg1	components					176:185	complex carbohydrate components	155:185	complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health	155:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	0	22	theme	Human	24:28	arg1	Trisaccharides					35:48	Fucosylated Human Milk Trisaccharides	12:48	Fucosylated Human Milk Trisaccharides in Biotechnological Context	12:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	6	23	theme	high	1064:1067	arg1	throughput					1069:1078	the high throughput	1060:1078	the high throughput of this technique	1060:1096	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	1	24	theme	Human	116:120	arg1	HMOs					145:148	HMOs	145:148	HMOs	145:148	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	24	theme	Human	116:120	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	24	theme	Human	116:120	arg1	components					176:185	complex carbohydrate components	155:185	complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health	155:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	0	25	theme	Fucosylated	12:22	arg1	Trisaccharides					35:48	Fucosylated Human Milk Trisaccharides	12:48	Fucosylated Human Milk Trisaccharides in Biotechnological Context	12:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	4	26	theme	encoded	633:639	arg1	biosensor					651:659	a genetically encoded bacterial biosensor	619:659	a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases	619:866	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	1	27	theme	milk	122:125	arg1	HMOs					145:148	HMOs	145:148	HMOs	145:148	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	27	theme	milk	122:125	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	27	theme	milk	122:125	arg1	components					176:185	complex carbohydrate components	155:185	complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health	155:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	6	28	theme	HMO	1233:1235	arg1	manufacturing					1237:1249	HMO manufacturing	1233:1249	HMO manufacturing	1233:1249	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	0	29	theme	Trisaccharides	35:48	arg1	Analysis					0:7	Analysis	0:7	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context	0:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	3	30	theme	chromatographic/mass-spectrometric	475:508	arg1	methods					510:516	chromatographic/mass-spectrometric methods	475:516	chromatographic/mass-spectrometric methods	475:516	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	1	31	theme	plentiful	221:229	arg1	benefits					231:238	plentiful benefits	221:238	plentiful benefits on infant health	221:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	0	32	theme	Milk	30:33	arg1	Trisaccharides					35:48	Fucosylated Human Milk Trisaccharides	12:48	Fucosylated Human Milk Trisaccharides in Biotechnological Context	12:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	3	33	theme	analysis	458:465	arg1	techniques					437:446	Conventional techniques	424:446	Conventional techniques of glycan analysis	424:465	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	6	34	theme	technique	1088:1096	arg1	throughput					1069:1078	the high throughput	1060:1078	the high throughput of this technique	1060:1096	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	5	35	theme	biotechnological	910:925	arg1	processes					927:935	biotechnological processes	910:935	biotechnological processes	910:935	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	5	36	theme	false	951:955	arg1	positives					957:965	false positives	951:965	false positives	951:965	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	0	37	theme	Biotechnological	53:68	arg1	Context					70:76	Biotechnological Context	53:76	Biotechnological Context	53:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	6	38	theme	bioreactor	1127:1136	arg1	parameters					1138:1147	bioreactor parameters	1127:1147	bioreactor parameters	1127:1147	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	0	39	from	Context	70:76	arg1	Analysis					0:7	Analysis	0:7	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context	0:76	Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors.					
31033942	1	40	from	benefits	231:238	arg1	health					250:255	infant health	243:255	infant health	243:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	2	41	theme	detection	363:371	arg1	throughput					349:358	the relatively low throughput	330:358	the relatively low throughput of detection and quantification of monosaccharide and linkages	330:421	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	5	42	from	presence	876:883	arg1	milk					899:902	milk	899:902	milk	899:902	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	5	42	from	presence	876:883	arg1	processes					927:935	biotechnological processes	910:935	biotechnological processes	910:935	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	5	43	attach	presence	876:883	arg1	milk					899:902	milk	899:902	milk	899:902	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	5	43	attach	presence	876:883	arg2	lactose					888:894	lactose	888:894	lactose	888:894	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	5	43	attach	presence	876:883	arg1	processes					927:935	biotechnological processes	910:935	biotechnological processes	910:935	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	3	44	theme	samples	562:568	arg1	hundreds					550:557	hundreds	550:557	hundreds of samples per day without automation	550:595	Conventional techniques of glycan analysis include chromatographic/mass-spectrometric methods with throughput on the order of hundreds of samples per day without automation.					
31033942	4	45	theme	structures	755:764	arg1	quantification					717:730	quantification	717:730	quantification	717:730	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	45	theme	structures	755:764	arg1	detection					703:711	detection	703:711	detection	703:711	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	46	theme	fucosidases	856:866	arg1	expression					842:851	heterologous expression	829:851	heterologous expression of fucosidases	829:866	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	2	47	theme	low	345:347	arg1	throughput					349:358	the relatively low throughput	330:358	the relatively low throughput of detection and quantification of monosaccharide and linkages	330:421	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	1	48	theme	infant	243:248	arg1	health					250:255	infant health	243:255	infant health	243:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	4	49	dep	high-throughput	669:683	arg1	linkage-specific					686:701	linkage-specific	686:701	linkage-specific	686:701	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	2	50	theme	monosaccharide	395:408	arg1	quantification					377:390	quantification	377:390	quantification	377:390	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	2	50	theme	monosaccharide	395:408	arg1	detection					363:371	detection	363:371	detection	363:371	However, optimization of their biotechnological synthesis is limited by the relatively low throughput of detection and quantification of monosaccharide and linkages.					
31033942	4	51	theme	fucosylated	739:749	arg1	3-fucosyllactose					789:804	3-fucosyllactose	789:804	3-fucosyllactose	789:804	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	51	theme	fucosylated	739:749	arg1	2'-fucosyllactose					767:783	2'-fucosyllactose	767:783	2'-fucosyllactose	767:783	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	4	51	theme	fucosylated	739:749	arg1	structures					755:764	the fucosylated HMO structures	735:764	the fucosylated HMO structures	735:764	We demonstrate here, a genetically encoded bacterial biosensor for the high-throughput, linkage-specific detection and quantification of the fucosylated HMO structures, 2'-fucosyllactose and 3-fucosyllactose, which we achieved via heterologous expression of fucosidases.					
31033942	6	52	theme	reaction	1104:1111	arg1	conditions					1113:1122	many reaction conditions	1099:1122	many reaction conditions	1099:1122	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	6	53	theme	hours	1193:1197	arg1	matter					1183:1188	a matter	1181:1188	a matter of hours	1181:1197	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
31033942	5	54	theme	lactose	888:894	arg1	presence					876:883	the presence	872:883	the presence of lactose in milk or in biotechnological processes	872:935	As the presence of lactose in milk or in biotechnological processes could lead to false positives, we also demonstrate the reduction of signal from lactose using different strategies.					
31033942	1	55	theme	complex	155:161	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	1	55	theme	complex	155:161	arg1	components					176:185	complex carbohydrate components	155:185	complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health	155:255	Human milk oligosaccharides (HMOs) are complex carbohydrate components of human breast milk that exhibit plentiful benefits on infant health.					
31033942	6	56	theme	many	1099:1102	arg1	conditions					1113:1122	many reaction conditions	1099:1122	many reaction conditions	1099:1122	Due to the high throughput of this technique, many reaction conditions or bioreactor parameters could be assayed in parallel in a matter of hours, allowing for the optimization of HMO manufacturing.					
29528515	2	0	theme	final	545:549	arg1	stage					561:565	the final synthetic stage	541:565	the final synthetic stage	541:565	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	1	theme	convergent	164:173	arg1	strategy					189:196	A highly convergent and efficient strategy	155:196	A highly convergent and efficient strategy	155:196	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	1	2	theme	Streptococcus	270:282	arg1	polysaccharides					311:325	Streptococcus pneumoniae type 3 capsular polysaccharides	270:325	Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	270:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	0	3	theme	Repeating	76:84	arg1	Unit					86:89	the Repeating Unit	72:89	the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	72:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	3	4	theme	non-reducing	742:753	arg1	terminus					755:762	the non-reducing terminus	738:762	the non-reducing terminus	738:762	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	0	5	theme	Streptococcus	94:106	arg1	Polysaccharide					139:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	4	6	theme	sugar	1014:1018	arg1	length					1026:1031	the sugar chain length	1010:1031	the sugar chain length	1010:1031	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	4	7	theme	T-cell-dependent	948:963	arg1	responses					991:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	1	8	contain	contain	332:338	arg2	units					365:369	multiple glucuronic acid units	340:369	multiple glucuronic acid units	340:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	1	8	contain	contain	332:338	arg1	polysaccharides					311:325	Streptococcus pneumoniae type 3 capsular polysaccharides	270:325	Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	270:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	1	9	theme	type	295:298	arg1	polysaccharides					311:325	Streptococcus pneumoniae type 3 capsular polysaccharides	270:325	Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	270:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	4	10	theme	immunological	1061:1073	arg1	properties					1075:1084	their immunological properties	1055:1084	their immunological properties	1055:1084	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	4	11	contain	had	1033:1035	arg2	impact					1045:1050	a major impact	1037:1050	a major impact	1037:1050	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	4	11	contain	had	1033:1035	arg1	length					1026:1031	the sugar chain length	1010:1031	the sugar chain length	1010:1031	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	3	12	contain	had	671:673	arg2	group					688:692	a free amino group	675:692	a free amino group	675:692	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	3	12	contain	had	671:673	arg1	oligosaccharides					654:669	The target oligosaccharides	643:669	The target oligosaccharides	643:669	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	0	13	theme	Serotype	119:126	arg1	Polysaccharide					139:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	3	14	theme	structure-activity	799:816	arg1	relationship					818:829	structure-activity relationship	799:829	structure-activity relationship	799:829	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	4	15	theme	G	980:980	arg1	responses					991:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	4	16	theme	oligosaccharide-tetanus	873:895	arg1	conjugates					904:913	the oligosaccharide-tetanus toxoid conjugates	869:913	the oligosaccharide-tetanus toxoid conjugates	869:913	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	1	17	theme	efficient	179:187	arg1	strategy					189:196	A highly convergent and efficient strategy	155:196	A highly convergent and efficient strategy	155:196	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	0	18	theme	pneumoniae	108:117	arg1	Polysaccharide					139:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	3	19	theme	further	774:780	arg1	modification					782:793	further modification	774:793	further modification	774:793	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	4	20	theme	immunoglobulin	965:978	arg1	responses					991:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	3	21	theme	modification	782:793	arg1	studies					831:837	further modification and structure-activity relationship studies	774:837	further modification and structure-activity relationship studies	774:837	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	2	22	theme	glucuronic	510:519	arg1	acid					521:524	glucuronic acid	510:524	glucuronic acid	510:524	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	23	theme	capsular	302:309	arg1	polysaccharides					311:325	Streptococcus pneumoniae type 3 capsular polysaccharides	270:325	Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	270:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	4	24	theme	antibody	982:989	arg1	responses					991:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	1	25	theme	polysaccharides	311:325	arg1	oligosaccharides					250:265	complex oligosaccharides	242:265	complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	242:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	0	26	theme	Capsular	130:137	arg1	Polysaccharide					139:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	94:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	4	27	theme	Immunological	840:852	arg1	evaluations					854:864	Immunological evaluations	840:864	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates	840:913	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	0	28	theme	Immunological	14:26	arg1	Studies					28:34	Immunological Studies	14:34	Immunological Studies	14:34	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	3	29	theme	relationship	818:829	arg1	studies					831:837	further modification and structure-activity relationship studies	774:837	further modification and structure-activity relationship studies	774:837	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	4	30	theme	major	1039:1043	arg1	impact					1045:1050	a major impact	1037:1050	a major impact	1037:1050	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	0	31	theme	Oligosaccharides	39:54	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	0	31	theme	Oligosaccharides	39:54	arg1	Studies					28:34	Immunological Studies	14:34	Immunological Studies	14:34	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	3	32	from	terminus	755:762	arg1	caps					730:733	caps	730:733	caps at the non-reducing terminus	730:762	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	1	33	theme	chemical	220:227	arg1	synthesis					229:237	the chemical synthesis	216:237	the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	216:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	2	34	theme	designated	451:460	arg1	units					470:474	the designated glucose units	447:474	the designated glucose units	447:474	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	35	theme	multiple	340:347	arg1	units					365:369	multiple glucuronic acid units	340:369	multiple glucuronic acid units	340:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	0	36	theme	Polysaccharide	139:152	arg1	Unit					86:89	the Repeating Unit	72:89	the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide	72:152	Synthesis and Immunological Studies of Oligosaccharides that Consist of the Repeating Unit of Streptococcus pneumoniae Serotype 3 Capsular Polysaccharide.					
29528515	2	37	from	stage	561:565	arg1	step					533:536	one step	529:536	one step	529:536	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	5	38	theme	promising	1152:1160	arg1	antigens					1162:1169	promising antigens	1152:1169	promising antigens for vaccine development	1152:1193	In particular, the penta- and hexasaccharides were identified as promising antigens for vaccine development.					
29528515	5	38	theme	promising	1152:1160	arg1	hexasaccharides					1117:1131	hexasaccharides	1117:1131	hexasaccharides	1117:1131	In particular, the penta- and hexasaccharides were identified as promising antigens for vaccine development.					
29528515	5	38	theme	promising	1152:1160	arg1	penta-					1106:1111	penta-	1106:1111	penta-	1106:1111	In particular, the penta- and hexasaccharides were identified as promising antigens for vaccine development.					
29528515	2	39	theme	glucose	462:468	arg1	units					470:474	the designated glucose units	447:474	the designated glucose units	447:474	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	40	theme	glucuronic	349:358	arg1	acid					360:363	glucuronic acid	349:363	multiple glucuronic acid units	340:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	3	41	theme	reducing	701:708	arg1	terminus					710:717	the reducing terminus	697:717	the reducing terminus	697:717	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	3	42	theme	target	647:652	arg1	oligosaccharides					654:669	The target oligosaccharides	643:669	The target oligosaccharides	643:669	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	2	43	theme	difficult	587:595	arg1	glycosylations					597:610	difficult glycosylations	587:610	difficult glycosylations that involved glucuronic acid	587:640	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	44	theme	acid	360:363	arg1	units					365:369	multiple glucuronic acid units	340:369	multiple glucuronic acid units	340:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	4	45	theme	chain	1020:1024	arg1	length					1026:1031	the sugar chain length	1010:1031	the sugar chain length	1010:1031	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	5	46	theme	vaccine	1175:1181	arg1	development					1183:1193	vaccine development	1175:1193	vaccine development	1175:1193	In particular, the penta- and hexasaccharides were identified as promising antigens for vaccine development.					
29528515	4	47	theme	robust	941:946	arg1	responses					991:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	robust T-cell-dependent immunoglobulin G antibody responses	941:999	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	4	48	theme	conjugates	904:913	arg1	evaluations					854:864	Immunological evaluations	840:864	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates	840:913	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	2	49	theme	glucuronic	626:635	arg1	acid					637:640	glucuronic acid	626:640	glucuronic acid	626:640	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	1	50	dep	Streptococcus	270:282	arg1	pneumoniae					284:293	pneumoniae	284:293	pneumoniae	284:293	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	4	51	theme	toxoid	897:902	arg1	conjugates					904:913	the oligosaccharide-tetanus toxoid conjugates	869:913	the oligosaccharide-tetanus toxoid conjugates	869:913	Immunological evaluations of the oligosaccharide-tetanus toxoid conjugates showed that they elicited robust T-cell-dependent immunoglobulin G antibody responses and that the sugar chain length had a major impact on their immunological properties.					
29528515	1	52	theme	complex	242:248	arg1	oligosaccharides					250:265	complex oligosaccharides	242:265	complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	242:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	3	53	theme	free	677:680	arg1	group					688:692	a free amino group	675:692	a free amino group	675:692	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
29528515	1	54	theme	oligosaccharides	250:265	arg1	synthesis					229:237	the chemical synthesis	216:237	the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units	216:369	A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides of Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units.					
29528515	2	55	theme	synthetic	551:559	arg1	stage					561:565	the final synthetic stage	541:565	the final synthetic stage	541:565	Once the oligoglucosides were efficiently and stereoselectively assembled, the designated glucose units were regioselectively oxidized to glucuronic acid in one step at the final synthetic stage, which helped avoid difficult glycosylations that involved glucuronic acid.					
29528515	3	56	theme	amino	682:686	arg1	group					688:692	a free amino group	675:692	a free amino group	675:692	The target oligosaccharides had a free amino group at the reducing terminus and varied caps at the non-reducing terminus to enable further modification and structure-activity relationship studies.					
30136532	10	0	from	advantage	1136:1144	arg1	analysis					1153:1160	the analysis	1149:1160	the analysis of sialylated glycans as well as isomeric oligosaccharides	1149:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	10	1	theme	glycans	1176:1182	arg1	analysis					1153:1160	the analysis	1149:1160	the analysis of sialylated glycans as well as isomeric oligosaccharides	1149:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	0	2	with	chromatography	34:47	arg1	phase					86:90	silica-hydride-based stationary phase	54:90	silica-hydride-based stationary phase	54:90	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	10	3	theme	oligosaccharides	1204:1219	arg1	analysis					1153:1160	the analysis	1149:1160	the analysis of sialylated glycans as well as isomeric oligosaccharides	1149:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	0	4	theme	N-glycosylation	96:110	arg1	characterization					112:127	N-glycosylation characterization	96:127	N-glycosylation characterization of monoclonal antibody drugs	96:156	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	10	5	theme	sialylated	1165:1174	arg1	glycans					1176:1182	sialylated glycans	1165:1182	sialylated glycans as well as isomeric oligosaccharides	1165:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	10	6	gly	sialylated	1165:1174	arg1	glycans					1176:1182	sialylated glycans	1165:1182	sialylated glycans as well as isomeric oligosaccharides	1165:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	7	7	theme	salt-free	845:853	arg1	phases					862:867	salt-free mobile phases	845:867	salt-free mobile phases	845:867	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	0	8	theme	monoclonal	132:141	arg1	antibody					143:150	monoclonal antibody	132:150	monoclonal antibody drugs	132:156	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	5	9	theme	liquid	618:623	arg1	spectrometry					645:656	liquid chromatography-mass spectrometry	618:656	liquid chromatography-mass spectrometry	618:656	Samples were analyzed by liquid chromatography-mass spectrometry after enzymatic cleavage, methylamidation, and purification.					
30136532	7	10	theme	high	823:826	arg1	resolution					828:837	high resolution	823:837	high resolution using salt-free mobile phases	823:867	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	9	11	theme	service	1038:1044	arg1	life					1046:1049	a long service life	1031:1049	a long service life	1031:1049	Its structure was more stable than that of HILIC, and it provided a long service life.					
30136532	4	12	theme	phase	561:565	arg1	chromatography					567:580	silica-hydride-based normal phase chromatography	533:580	silica-hydride-based normal phase chromatography (SiH-NPC)	533:590	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	4	12	theme	phase	561:565	arg1	SiH-NPC					583:589	SiH-NPC	583:589	SiH-NPC	583:589	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	0	13	theme	drugs	152:156	arg1	characterization					112:127	N-glycosylation characterization	96:127	N-glycosylation characterization of monoclonal antibody drugs	96:156	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	4	14	theme	normal	554:559	arg1	chromatography					567:580	silica-hydride-based normal phase chromatography	533:580	silica-hydride-based normal phase chromatography (SiH-NPC)	533:590	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	4	14	theme	normal	554:559	arg1	SiH-NPC					583:589	SiH-NPC	583:589	SiH-NPC	583:589	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	11	15	theme	potential	1282:1290	arg1	applications					1292:1303	potential applications	1282:1303	potential applications	1282:1303	The approach has potential applications in the biopharmaceutical industry.					
30136532	1	16	theme	efficacious	193:203	arg1	agents					217:222	efficacious therapeutic agents	193:222	efficacious therapeutic agents against various complex diseases	193:255	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	1	16	theme	efficacious	193:203	arg1	antibodies					171:180	Monoclonal antibodies	160:180	Monoclonal antibodies (mAbs)	160:187	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	0	17	theme	antibody	143:150	arg1	drugs					152:156	monoclonal antibody drugs	132:156	monoclonal antibody drugs	132:156	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	2	18	theme	fluorescence	321:332	arg1	derivatization					334:347	fluorescence derivatization	321:347	fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC)	321:414	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	3	19	contain	has	438:440	arg2	drawbacks					447:455	some drawbacks	442:455	some drawbacks	442:455	However, this method has some drawbacks.					
30136532	3	19	contain	has	438:440	arg1	method					431:436	this method	426:436	this method	426:436	However, this method has some drawbacks.					
30136532	10	20	theme	isomeric	1195:1202	arg1	oligosaccharides					1204:1219	isomeric oligosaccharides	1195:1219	sialylated glycans as well as isomeric oligosaccharides	1165:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	10	21	with	combination	1055:1065	arg1	derivatization					1088:1101	the sialic acid derivatization	1072:1101	the sialic acid derivatization	1072:1101	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	2	22	theme	liquid	386:391	arg1	HILIC					409:413	HILIC	409:413	HILIC	409:413	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	2	22	theme	liquid	386:391	arg1	chromatography					393:406	hydrophilic interaction liquid chromatography	362:406	hydrophilic interaction liquid chromatography (HILIC)	362:414	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	0	23	theme	phase	28:32	arg1	chromatography					34:47	normal phase chromatography	21:47	normal phase chromatography	21:47	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	10	24	theme	chromatography-mass	1231:1249	arg1	spectrometry					1251:1262	liquid chromatography-mass spectrometry	1224:1262	liquid chromatography-mass spectrometry	1224:1262	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	2	25	theme	interaction	374:384	arg1	HILIC					409:413	HILIC	409:413	HILIC	409:413	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	2	25	theme	interaction	374:384	arg1	chromatography					393:406	hydrophilic interaction liquid chromatography	362:406	hydrophilic interaction liquid chromatography (HILIC)	362:414	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	1	26	theme	various	232:238	arg1	diseases					248:255	various complex diseases	232:255	various complex diseases	232:255	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	0	27	theme	normal	21:26	arg1	chromatography					34:47	normal phase chromatography	21:47	normal phase chromatography	21:47	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	7	28	theme	spectrometer	905:916	arg1	contamination					879:891	contamination	879:891	contamination of the mass spectrometer	879:916	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	10	29	theme	significant	1124:1134	arg1	advantage					1136:1144	a significant advantage	1122:1144	a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides	1122:1219	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	2	30	theme	hydrophilic	362:372	arg1	HILIC					409:413	HILIC	409:413	HILIC	409:413	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	2	30	theme	hydrophilic	362:372	arg1	chromatography					393:406	hydrophilic interaction liquid chromatography	362:406	hydrophilic interaction liquid chromatography (HILIC)	362:414	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	1	31	theme	complex	240:246	arg1	diseases					248:255	various complex diseases	232:255	various complex diseases	232:255	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	2	32	theme	mAbs	294:297	arg1	characterization					274:289	N-glycosylation characterization	258:289	N-glycosylation characterization of mAbs	258:297	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	7	33	theme	novel	779:783	arg1	mechanism					796:804	a novel separation mechanism	777:804	a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer	777:916	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	4	34	theme	silica-hydride-based	533:552	arg1	chromatography					567:580	silica-hydride-based normal phase chromatography	533:580	silica-hydride-based normal phase chromatography (SiH-NPC)	533:590	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	4	34	theme	silica-hydride-based	533:552	arg1	SiH-NPC					583:589	SiH-NPC	583:589	SiH-NPC	583:589	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	11	35	contain	has	1278:1280	arg1	approach					1269:1276	The approach	1265:1276	The approach	1265:1276	The approach has potential applications in the biopharmaceutical industry.					
30136532	11	35	contain	has	1278:1280	arg2	applications					1292:1303	potential applications	1282:1303	potential applications	1282:1303	The approach has potential applications in the biopharmaceutical industry.					
30136532	5	36	theme	chromatography-mass	625:643	arg1	spectrometry					645:656	liquid chromatography-mass spectrometry	618:656	liquid chromatography-mass spectrometry	618:656	Samples were analyzed by liquid chromatography-mass spectrometry after enzymatic cleavage, methylamidation, and purification.					
30136532	0	37	theme	stationary	75:84	arg1	phase					86:90	silica-hydride-based stationary phase	54:90	silica-hydride-based stationary phase	54:90	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	7	38	theme	mass	900:903	arg1	spectrometer					905:916	the mass spectrometer	896:916	the mass spectrometer	896:916	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	6	39	contain	had	727:729	arg2	advantages					736:745	some advantages	731:745	some advantages	731:745	SiH-NPC had some advantages compared to HILIC.					
30136532	6	39	contain	had	727:729	arg1	SiH-NPC					719:725	SiH-NPC	719:725	SiH-NPC	719:725	SiH-NPC had some advantages compared to HILIC.					
30136532	1	40	dep	efficacious	193:203	arg1	therapeutic					205:215	therapeutic	205:215	therapeutic	205:215	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	0	41	theme	silica-hydride-based	54:73	arg1	phase					86:90	silica-hydride-based stationary phase	54:90	silica-hydride-based stationary phase	54:90	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	4	42	theme	mAbs	492:495	arg1	N-glycosylation					473:487	the N-glycosylation	469:487	the N-glycosylation of mAbs	469:495	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
30136532	2	43	theme	N-glycosylation	258:272	arg1	characterization					274:289	N-glycosylation characterization	258:289	N-glycosylation characterization of mAbs	258:297	N-glycosylation characterization of mAbs is based primarily on fluorescence derivatization coupled with hydrophilic interaction liquid chromatography (HILIC).					
30136532	7	44	theme	separation	785:794	arg1	mechanism					796:804	a novel separation mechanism	777:804	a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer	777:916	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	0	45	with	[Methylamidation	0:15	arg1	phase					86:90	silica-hydride-based stationary phase	54:90	silica-hydride-based stationary phase	54:90	[Methylamidation and normal phase chromatography with silica-hydride-based stationary phase for N-glycosylation characterization of monoclonal antibody drugs].					
30136532	1	46	theme	Monoclonal	160:169	arg1	agents					217:222	efficacious therapeutic agents	193:222	efficacious therapeutic agents against various complex diseases	193:255	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	1	46	theme	Monoclonal	160:169	arg1	mAbs					183:186	mAbs	183:186	mAbs	183:186	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	1	46	theme	Monoclonal	160:169	arg1	antibodies					171:180	Monoclonal antibodies	160:180	Monoclonal antibodies (mAbs)	160:187	Monoclonal antibodies (mAbs) are efficacious therapeutic agents against various complex diseases.					
30136532	11	47	theme	biopharmaceutical	1312:1328	arg1	industry					1330:1337	the biopharmaceutical industry	1308:1337	the biopharmaceutical industry	1308:1337	The approach has potential applications in the biopharmaceutical industry.					
30136532	5	48	theme	enzymatic	664:672	arg1	cleavage					674:681	enzymatic cleavage	664:681	enzymatic cleavage	664:681	Samples were analyzed by liquid chromatography-mass spectrometry after enzymatic cleavage, methylamidation, and purification.					
30136532	10	49	theme	liquid	1224:1229	arg1	spectrometry					1251:1262	liquid chromatography-mass spectrometry	1224:1262	liquid chromatography-mass spectrometry	1224:1262	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	8	50	theme	rapid	949:953	arg1	analysis					955:962	rapid analysis	949:962	rapid analysis	949:962	SiH-NPC may be applicable for rapid analysis.					
30136532	9	51	theme	long	1033:1036	arg1	life					1046:1049	a long service life	1031:1049	a long service life	1031:1049	Its structure was more stable than that of HILIC, and it provided a long service life.					
30136532	10	52	attach	presented	1112:1120	arg2	SiH-NPC					1104:1110	SiH-NPC	1104:1110	SiH-NPC	1104:1110	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	10	52	attach	presented	1112:1120	arg1	combination					1055:1065	combination	1055:1065	combination with the sialic acid derivatization	1055:1101	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	10	53	theme	acid	1083:1086	arg1	derivatization					1088:1101	the sialic acid derivatization	1072:1101	the sialic acid derivatization	1072:1101	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	7	54	theme	mobile	855:860	arg1	phases					862:867	salt-free mobile phases	845:867	salt-free mobile phases	845:867	It offered a novel separation mechanism that allowed for high resolution using salt-free mobile phases, avoiding contamination of the mass spectrometer.					
30136532	10	55	theme	sialic	1076:1081	arg1	derivatization					1088:1101	the sialic acid derivatization	1072:1101	the sialic acid derivatization	1072:1101	In combination with the sialic acid derivatization, SiH-NPC presented a significant advantage in the analysis of sialylated glycans as well as isomeric oligosaccharides by liquid chromatography-mass spectrometry.					
30136532	4	56	gly	N-glycosylation	473:487	arg1	mAbs					492:495	mAbs	492:495	mAbs	492:495	Presently, the N-glycosylation of mAbs was analyzed by methylamidation and silica-hydride-based normal phase chromatography (SiH-NPC).					
31823247	0	0	theme	rich	87:90	arg1	yeasts					92:97	rich yeasts'	87:98	ß-glucan rich yeasts' surfaces	78:107	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	0	1	from	profiling	12:20	arg1	microorganisms					50:63	microorganisms	50:63	microorganisms	50:63	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	3	2	theme	Lens	734:737	arg1	lectin					749:754	Lens culinaris lectin	734:754	Lens culinaris lectin	734:754	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	6	3	theme	specific	1250:1257	arg1	binding					1259:1265	specific binding	1250:1265	specific binding to all of the tested fungal species	1250:1301	Recombinant banana lectin showed specific binding to all of the tested fungal species.					
31823247	3	4	theme	lectin	531:536	arg1	assay					546:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	5	5	theme	casei	1207:1211	arg1	DG					1213:1214	Lactobacillus casei DG	1193:1214	Lactobacillus casei DG	1193:1214	Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG.					
31823247	0	6	theme	ß-glucan	78:85	arg1	surfaces					100:107	ß-glucan rich yeasts' surfaces	78:107	ß-glucan rich yeasts' surfaces	78:107	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	3	7	theme	sorbent	538:544	arg1	assay					546:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	8	theme	Ricinus	801:807	arg1	agglutinin					818:827	Ricinus communis agglutinin	801:827	Ricinus communis agglutinin	801:827	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	6	9	theme	banana	1229:1234	arg1	lectin					1236:1241	Recombinant banana lectin	1217:1241	Recombinant banana lectin	1217:1241	Recombinant banana lectin showed specific binding to all of the tested fungal species.					
31823247	1	10	theme	receptor	276:283	arg1	structures					285:294	receptor structures	276:294	receptor structures on host's cells and antibodies	276:325	The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies.					
31823247	4	11	with	interaction	1016:1026	arg1	lectins					1052:1058	the selected plant lectins	1033:1058	the selected plant lectins	1033:1058	The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL.					
31823247	6	12	theme	Recombinant	1217:1227	arg1	lectin					1236:1241	Recombinant banana lectin	1217:1241	Recombinant banana lectin	1217:1241	Recombinant banana lectin showed specific binding to all of the tested fungal species.					
31823247	8	13	with	ß-glucan	1547:1554	arg1	to					1594:1595	to	1594:1595	to	1594:1595	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	8	13	with	ß-glucan	1547:1554	arg1	1.81 μg mL-1					1577:1588	IC50 1.81 μg mL-1	1572:1588	IC50 1.81 μg mL-1	1572:1588	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	3	14	theme	Sambucus	865:872	arg1	ensiformis					853:862	Canavalia ensiformis	843:862	Canavalia ensiformis	843:862	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	14	theme	Sambucus	865:872	arg1	agglutinin					880:889	Sambucus nigra agglutinin	865:889	Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	865:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	8	15	theme	IC50	1572:1575	arg1	1.81 μg mL-1					1577:1588	IC50 1.81 μg mL-1	1572:1588	IC50 1.81 μg mL-1	1572:1588	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	4	16	theme	selected	1037:1044	arg1	lectins					1052:1058	the selected plant lectins	1033:1058	the selected plant lectins	1033:1058	The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL.					
31823247	7	17	theme	Candida	1325:1331	arg1	albicans					1333:1340	Candida albicans	1325:1340	Candida albicans	1325:1340	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	2	18	theme	surface	398:404	arg1	glycosylation					406:418	surface glycosylation	398:418	surface glycosylation	398:418	The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms.					
31823247	3	19	theme	bean	711:714	arg1	agglutinin					716:725	Soy bean agglutinin	707:725	Soy bean agglutinin	707:725	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	19	theme	bean	711:714	arg1	A					834:834	Con A	830:834	Con A	830:834	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	7	20	theme	fluorescence	1366:1377	arg1	microscopy					1379:1388	fluorescence microscopy	1366:1388	fluorescence microscopy	1366:1388	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	0	21	theme	based	6:10	arg1	profiling					12:20	ELLSA based profiling	0:20	ELLSA based profiling of surface glycosylation in microorganisms	0:63	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	2	22	theme	different	491:499	arg1	microorganisms					501:514	different microorganisms	491:514	different microorganisms	491:514	The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms.					
31823247	5	23	theme	tested	1104:1109	arg1	lectins					1111:1117	the tested lectins	1100:1117	the tested lectins	1100:1117	Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG.					
31823247	8	24	with	1.81 μg mL-1	1577:1588	arg1	1.10 μg mL-1					1616:1627	1.10 μg mL-1	1616:1627	1.10 μg mL-1	1616:1627	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	1	25	theme	microorganisms	183:196	arg1	surface					172:178	The surface	168:178	The surface of microorganisms	168:196	The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies.					
31823247	0	26	theme	ELLSA	0:4	arg1	profiling					12:20	ELLSA based profiling	0:20	ELLSA based profiling of surface glycosylation in microorganisms	0:63	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	3	27	theme	culinaris	739:747	arg1	lectin					749:754	Lens culinaris lectin	734:754	Lens culinaris lectin	734:754	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	28	theme	SNA	893:895	arg1	I					897:897	SNA I and Maackia amurensis agglutinin	893:930	I	897:897	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	29	used	used	564:567	arg2	ELLSA					554:558	ELLSA	554:558	ELLSA	554:558	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	29	used	used	564:567	arg2	assay					546:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	4	30	theme	surface	982:988	arg1	glycosylation					990:1002	the microorganisms' surface glycosylation	962:1002	the microorganisms' surface glycosylation by means of interaction with the selected plant lectins	962:1058	The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL.					
31823247	6	31	theme	fungal	1288:1293	arg1	species					1295:1301	the tested fungal species	1277:1301	the tested fungal species	1277:1301	Recombinant banana lectin showed specific binding to all of the tested fungal species.					
31823247	7	32	theme	BL	1319:1320	arg1	binding					1308:1314	The binding	1304:1314	The binding of BL to Candida albicans	1304:1340	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	4	33	theme	plant	1046:1050	arg1	lectins					1052:1058	the selected plant lectins	1033:1058	the selected plant lectins	1033:1058	The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL.					
31823247	5	34	theme	most	1119:1122	arg1	binding					1134:1140	most selective binding	1119:1140	most selective binding	1119:1140	Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG.					
31823247	1	35	with	contact	263:269	arg1	structures					285:294	receptor structures	276:294	receptor structures on host's cells and antibodies	276:325	The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies.					
31823247	9	36	from	detection	1726:1734	arg1	solutions					1751:1759	solutions	1751:1759	solutions	1751:1759	This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.					
31823247	5	37	theme	selective	1124:1132	arg1	binding					1134:1140	most selective binding	1119:1140	most selective binding	1119:1140	Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG.					
31823247	7	38	theme	rich	1474:1477	arg1	surfaces					1479:1486	ß-glucan rich surfaces	1465:1486	ß-glucan rich surfaces	1465:1486	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	0	39	theme	glycosylation	33:45	arg1	profiling					12:20	ELLSA based profiling	0:20	ELLSA based profiling of surface glycosylation in microorganisms	0:63	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	3	40	theme	nigra	874:878	arg1	ensiformis					853:862	Canavalia ensiformis	843:862	Canavalia ensiformis	843:862	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	40	theme	nigra	874:878	arg1	agglutinin					880:889	Sambucus nigra agglutinin	865:889	Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	865:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	0	41	theme	recombinant	141:151	arg1	lectin					160:165	recombinant banana lectin	141:165	recombinant banana lectin	141:165	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	3	42	theme	acuminata	609:617	arg1	lectin					619:624	recombinant Musa acuminata lectin	592:624	recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	592:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	agglutinin					716:725	Soy bean agglutinin	707:725	Soy bean agglutinin	707:725	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	agglutinin					818:827	Ricinus communis agglutinin	801:827	Ricinus communis agglutinin	801:827	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	SBA					729:731	SBA	729:731	SBA	729:731	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	RCA120					792:797	RCA120	792:797	RCA120	792:797	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	A					834:834	Con A	830:834	Con A	830:834	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	lectin					749:754	Lens culinaris lectin	734:754	Lens culinaris lectin	734:754	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	agglutinin					774:783	Wheat germ agglutinin	763:783	Wheat germ agglutinin	763:783	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	43	dep	lectin	619:624	arg1	used					686:689	used	686:689	were used for comparison	681:704	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	1	44	theme	polysaccharide	214:227	arg1	structures					229:238	polysaccharide structures	214:238	polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies	214:325	The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies.					
31823247	0	45	theme	surface	25:31	arg1	glycosylation					33:45	surface glycosylation	25:45	surface glycosylation	25:45	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	2	46	theme	lectins	478:484	arg1	interaction					457:467	the interaction	453:467	the interaction of plant lectins with different microorganisms	453:514	The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms.					
31823247	3	47	theme	selected	637:644	arg1	microorganisms					646:659	27 selected microorganisms	634:659	27 selected microorganisms	634:659	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	48	theme	lectin	619:624	arg1	binding					581:587	the binding	577:587	the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	577:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	49	theme	amurensis	911:919	arg1	agglutinin					921:930	SNA I and Maackia amurensis agglutinin	893:930	agglutinin	921:930	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	8	50	from	yeast	1561:1565	arg1	ß-glucan					1547:1554	ß-glucan	1547:1554	ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1	1547:1627	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	2	51	theme	plant	472:476	arg1	lectins					478:484	plant lectins	472:484	plant lectins	472:484	The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms.					
31823247	3	52	theme	Wheat	763:767	arg1	agglutinin					774:783	Wheat germ agglutinin	763:783	Wheat germ agglutinin	763:783	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	53	theme	recombinant	592:602	arg1	lectin					619:624	recombinant Musa acuminata lectin	592:624	recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	592:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	7	54	theme	flow	1394:1397	arg1	cytometry					1399:1407	flow cytometry	1394:1407	flow cytometry	1394:1407	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	4	55	with	comparison	1074:1083	arg1	BL					1090:1091	BL	1090:1091	BL	1090:1091	The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL.					
31823247	9	56	theme	BL	1657:1658	arg1	specificity					1642:1652	This unique specificity	1630:1652	This unique specificity of BL	1630:1658	This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.					
31823247	3	57	used	used	686:689	arg2	BL					628:629	BL	628:629	BL to 27 selected microorganisms and 7 other lectins	628:679	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	0	58	theme	banana	153:158	arg1	lectin					160:165	recombinant banana lectin	141:165	recombinant banana lectin	141:165	ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.					
31823247	8	59	theme	BL	1504:1505	arg1	binding					1493:1499	The binding	1489:1499	The binding of BL to S. boulardii	1489:1521	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	3	60	theme	Musa	604:607	arg1	lectin					619:624	recombinant Musa acuminata lectin	592:624	recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	592:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	2	61	with	interaction	457:467	arg1	microorganisms					501:514	different microorganisms	491:514	different microorganisms	491:514	The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms.					
31823247	3	62	theme	Enzyme-linked	517:529	arg1	assay					546:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	9	63	theme	unique	1635:1640	arg1	specificity					1642:1652	This unique specificity	1630:1652	This unique specificity of BL	1630:1658	This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.					
31823247	7	64	theme	ß-glucan	1465:1472	arg1	surfaces					1479:1486	ß-glucan rich surfaces	1465:1486	ß-glucan rich surfaces	1465:1486	The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces.					
31823247	3	65	from	ensiformis	853:862	arg1	lectin					619:624	recombinant Musa acuminata lectin	592:624	recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	592:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	65	from	ensiformis	853:862	arg1	binding					581:587	the binding	577:587	the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	577:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	66	theme	Maackia	903:909	arg1	agglutinin					921:930	SNA I and Maackia amurensis agglutinin	893:930	agglutinin	921:930	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	67	theme	Con	830:832	arg1	agglutinin					716:725	Soy bean agglutinin	707:725	Soy bean agglutinin	707:725	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	67	theme	Con	830:832	arg1	A					834:834	Con A	830:834	Con A	830:834	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	68	link	Enzyme-linked	517:529	arg1	assay					546:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay	517:550	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	69	theme	other	667:671	arg1	lectins					673:679	7 other lectins	665:679	7 other lectins	665:679	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	6	70	theme	tested	1281:1286	arg1	species					1295:1301	the tested fungal species	1277:1301	the tested fungal species	1277:1301	Recombinant banana lectin showed specific binding to all of the tested fungal species.					
31823247	3	71	dep	agglutinin	880:889	arg1	MAA					934:936	MAA	934:936	Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	865:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	71	dep	agglutinin	880:889	arg1	agglutinin					921:930	SNA I and Maackia amurensis agglutinin	893:930	agglutinin	921:930	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	71	dep	agglutinin	880:889	arg1	I					897:897	SNA I and Maackia amurensis agglutinin	893:930	I	897:897	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	72	from	binding	581:587	arg1	ensiformis					853:862	Canavalia ensiformis	843:862	Canavalia ensiformis	843:862	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	72	from	binding	581:587	arg1	agglutinin					880:889	Sambucus nigra agglutinin	865:889	Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA	865:936	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	9	73	theme	screening	1683:1691	arg1	purposes					1693:1700	screening purposes	1683:1700	screening purposes	1683:1700	This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.					
31823247	3	74	dep	Ricinus	801:807	arg1	communis					809:816	communis	809:816	communis	809:816	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	75	theme	germ	769:772	arg1	agglutinin					774:783	Wheat germ agglutinin	763:783	Wheat germ agglutinin	763:783	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	8	76	dep	1.81 μg mL-1	1577:1588	arg1	roqueforti					1600:1609	roqueforti	1600:1609	roqueforti	1600:1609	The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL-1 and to P. roqueforti with 1.10 μg mL-1.					
31823247	3	77	theme	Soy	707:709	arg1	agglutinin					716:725	Soy bean agglutinin	707:725	Soy bean agglutinin	707:725	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	3	77	theme	Soy	707:709	arg1	A					834:834	Con A	830:834	Con A	830:834	Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA.					
31823247	9	78	theme	ß-glucan	1739:1746	arg1	detection					1726:1734	the detection	1722:1734	the detection of ß-glucan in solutions	1722:1759	This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.					
31823247	5	79	theme	Lactobacillus	1193:1205	arg1	casei					1207:1211	Lactobacillus casei	1193:1211	Lactobacillus casei DG	1193:1214	Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG.					
31823247	1	80	theme	immediate	253:261	arg1	contact					263:269	immediate contact	253:269	immediate contact with receptor structures on host's cells and antibodies	253:325	The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies.					
30673210	4	0	theme	serum	900:904	arg1	BSA					915:917	BSA	915:917	BSA	915:917	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	0	theme	serum	900:904	arg1	albumin					906:912	bovine serum albumin	893:912	bovine serum albumin (BSA)	893:918	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	7	1	gly	glycopeptides	1437:1449	arg2	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	1	gly	glycopeptides	1437:1449	arg1	proteins					1473:1480	146 glycosylated proteins	1456:1480	146 glycosylated proteins	1456:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	2	theme	unique	1430:1435	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	6	3	theme	albumin	1257:1263	arg1	digest					1273:1278	bovine serum albumin trypsin digest	1244:1278	bovine serum albumin trypsin digest as low as 1/500	1244:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	9	4	contain	had	1786:1788	arg1	membrane					1755:1762	the chitosan-based membrane	1736:1762	the chitosan-based membrane prepared in this work	1736:1784	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	9	4	contain	had	1786:1788	arg2	potential					1796:1804	great potential	1790:1804	great potential for pretreatment of samples in glycoproteomics	1790:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	3	5	theme	freeze-casting	757:770	arg1	method					772:777	the freeze-casting method	753:777	the freeze-casting method	753:777	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	6	6	theme	bovine	1244:1249	arg1	albumin					1257:1263	bovine serum albumin	1244:1263	bovine serum albumin trypsin digest as low as 1/500	1244:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	7	7	theme	N-glycosylation	1401:1415	arg1	sites					1417:1421	270 unique N-glycosylation sites	1390:1421	270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins	1390:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	7	theme	N-glycosylation	1401:1415	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	6	8	theme	digest	1234:1239	arg1	ratio					1213:1217	A molar ratio	1205:1217	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500	1205:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	2	9	theme	interaction	355:365	arg1	HILIC					383:387	HILIC	383:387	HILIC	383:387	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	2	9	theme	interaction	355:365	arg1	chromatography					367:380	hydrophilic interaction chromatography	343:380	hydrophilic interaction chromatography (HILIC)	343:388	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	1	10	theme	samples	203:209	arg1	pretreatments					186:198	certain pretreatments	178:198	certain pretreatments of samples	178:209	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	4	11	theme	proteins	931:938	arg1	digests					839:845	trypsin digests	831:845	trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	831:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	2	12	theme	hydrophilic	593:603	arg1	groups					605:610	high-density hydrophilic groups	580:610	high-density hydrophilic groups	580:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	6	13	theme	trypsin	1226:1232	arg1	digest					1234:1239	IgG trypsin digest	1222:1239	IgG trypsin digest	1222:1239	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	0	14	theme	Biomimetic	22:31	arg1	Membrane					42:49	Biomimetic Chitosan Membrane	22:49	Biomimetic Chitosan Membrane	22:49	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	1	15	gly	glycopeptides	309:321	arg2	glycopeptides					309:321	glycopeptides detection	309:331	glycopeptides detection	309:331	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	9	16	theme	chitosan-based	1740:1753	arg1	membrane					1755:1762	the chitosan-based membrane	1736:1762	the chitosan-based membrane prepared in this work	1736:1784	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	3	17	theme	1	629:629	arg1	mm					631:632	mm	631:632	mm	631:632	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	6	18	theme	molar	1207:1211	arg1	ratio					1213:1217	A molar ratio	1205:1217	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500	1205:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	6	19	theme	low	1283:1285	arg1	digest					1273:1278	bovine serum albumin trypsin digest	1244:1278	bovine serum albumin trypsin digest as low as 1/500	1244:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	3	20	theme	membrane	670:677	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	20	theme	membrane	670:677	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	1	21	theme	glycoproteomics	138:152	arg1	study					129:133	the study	125:133	the study of glycoproteomics with mass spectrometry	125:175	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	4	22	gly	N-glycopeptides	810:824	arg2	N-glycopeptides					810:824	The N-glycopeptides	806:824	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	806:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	22	gly	N-glycopeptides	810:824	arg1	digests					839:845	trypsin digests	831:845	trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	831:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	3	23	theme	honeycomb	651:659	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	23	theme	honeycomb	651:659	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	1	24	theme	mass	159:162	arg1	spectrometry					164:175	mass spectrometry	159:175	mass spectrometry	159:175	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	4	25	theme	commercial	994:1003	arg1	material					1005:1012	a commercial material ZIC-HILIC	992:1022	a commercial material ZIC-HILIC	992:1022	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	9	26	from	glycoproteomics	1837:1851	arg1	pretreatment					1810:1821	pretreatment	1810:1821	pretreatment of samples in glycoproteomics	1810:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	2	27	with	cost	537:540	arg1	modifications					563:575	modifications	563:575	modifications of high-density hydrophilic groups	563:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	8	28	gly	deglycosylated	1677:1690	arg1	digest					1699:1704	30 μg deglycosylated fetuin digest	1671:1704	30 μg deglycosylated fetuin digest	1671:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	9	29	from	pretreatment	1810:1821	arg1	glycoproteomics					1837:1851	glycoproteomics	1837:1851	glycoproteomics	1837:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	0	30	theme	Glycosylated	99:110	arg1	Peptides					112:119	Glycosylated Peptides	99:119	Glycosylated Peptides	99:119	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	1	31	gly	nonglycopeptides	260:275	arg2	nonglycopeptides					260:275	nonglycopeptides	260:275	nonglycopeptides	260:275	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	4	32	theme	trypsin	831:837	arg1	digests					839:845	trypsin digests	831:845	trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	831:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	1	33	with	glycoproteomics	138:152	arg1	spectrometry					164:175	mass spectrometry	159:175	mass spectrometry	159:175	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	9	34	theme	samples	1826:1832	arg1	pretreatment					1810:1821	pretreatment	1810:1821	pretreatment of samples in glycoproteomics	1810:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	2	35	theme	glycosylated	427:438	arg1	peptides					440:447	glycosylated peptides	427:447	glycosylated peptides	427:447	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	0	36	theme	Facile	0:5	arg1	Fabrication					7:17	Facile Fabrication	0:17	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.	0:120	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	5	37	theme	wide	1165:1168	arg1	scope					1170:1174	a wide scope	1163:1174	a wide scope for glycopeptide enrichment	1163:1202	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	6	38	gly	glycopeptide	1352:1363	arg2	glycopeptide					1352:1363	glycopeptide enrichment	1352:1374	glycopeptide enrichment	1352:1374	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	2	39	theme	hydrophilic	464:474	arg1	materials					476:484	hydrophilic materials	464:484	hydrophilic materials	464:484	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	1	40	theme	detection	323:331	arg1	efficiency					295:304	the efficiency	291:304	the efficiency of glycopeptides detection	291:331	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	7	41	theme	serum	1541:1545	arg1	analysis					1518:1525	the triplicate analysis	1503:1525	the triplicate analysis of 2 μL human serum	1503:1545	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	4	42	theme	ZIC-HILIC	1014:1022	arg1	material					1005:1012	a commercial material ZIC-HILIC	992:1022	a commercial material ZIC-HILIC	992:1022	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	0	43	theme	Chitosan	33:40	arg1	Membrane					42:49	Biomimetic Chitosan Membrane	22:49	Biomimetic Chitosan Membrane	22:49	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	7	44	theme	μL	1532:1533	arg1	serum					1541:1545	2 μL human serum	1530:1545	2 μL human serum	1530:1545	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	8	45	gly	O-glycopeptides	1607:1621	arg2	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	2	46	theme	steric	513:518	arg1	hindrance					520:528	large steric hindrance	507:528	large steric hindrance	507:528	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	8	47	theme	O-glycosylation	1571:1585	arg1	sites					1587:1591	48 unique O-glycosylation sites	1561:1591	48 unique O-glycosylation sites of 278 unique O-glycopeptides	1561:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	47	theme	O-glycosylation	1571:1585	arg1	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	7	48	theme	triplicate	1507:1516	arg1	analysis					1518:1525	the triplicate analysis	1503:1525	the triplicate analysis of 2 μL human serum	1503:1545	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	8	49	theme	unique	1600:1605	arg1	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	3	50	theme	accessible	706:715	arg1	macropores					717:726	honeycomb-like accessible macropores	691:726	honeycomb-like accessible macropores	691:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	4	51	theme	G	865:865	arg1	digests					839:845	trypsin digests	831:845	trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	831:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	8	52	theme	μg	1674:1675	arg1	digest					1699:1704	30 μg deglycosylated fetuin digest	1671:1704	30 μg deglycosylated fetuin digest	1671:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	7	53	gly	N-glycosylation	1401:1415	arg2	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	53	gly	N-glycosylation	1401:1415	arg2	proteins					1473:1480	146 glycosylated proteins	1456:1480	146 glycosylated proteins	1456:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	53	gly	N-glycosylation	1401:1415	arg2	sites					1417:1421	270 unique N-glycosylation sites	1390:1421	270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins	1390:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	53	gly	N-glycosylation	1401:1415	arg2	270					1390:1392	270	1390:1392	270	1390:1392	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	53	gly	N-glycosylation	1401:1415	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	8	54	theme	fetuin	1692:1697	arg1	digest					1699:1704	30 μg deglycosylated fetuin digest	1671:1704	30 μg deglycosylated fetuin digest	1671:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	4	55	theme	bovine	893:898	arg1	BSA					915:917	BSA	915:917	BSA	915:917	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	55	theme	bovine	893:898	arg1	albumin					906:912	bovine serum albumin	893:912	bovine serum albumin (BSA)	893:918	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	56	theme	serum	925:929	arg1	proteins					931:938	serum proteins	925:938	serum proteins	925:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	6	57	theme	trypsin	1265:1271	arg1	digest					1273:1278	bovine serum albumin trypsin digest	1244:1278	bovine serum albumin trypsin digest as low as 1/500	1244:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	4	58	from	digests	839:845	arg1	N-glycopeptides					810:824	The N-glycopeptides	806:824	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	806:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	7	59	theme	glycosylated	1460:1471	arg1	proteins					1473:1480	146 glycosylated proteins	1456:1480	146 glycosylated proteins	1456:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	4	60	theme	albumin	906:912	arg1	proteins					931:938	serum proteins	925:938	serum proteins	925:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	60	theme	albumin	906:912	arg1	mixture					874:880	mixture	874:880	mixture of IgG and bovine serum albumin (BSA)	874:918	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	60	theme	albumin	906:912	arg1	G					865:865	immunoglobulin G	850:865	immunoglobulin G (IgG)	850:871	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	60	theme	albumin	906:912	arg1	IgG					868:870	IgG	868:870	IgG	868:870	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	7	61	theme	glycopeptides	1437:1449	arg1	sites					1417:1421	270 unique N-glycosylation sites	1390:1421	270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins	1390:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	61	theme	glycopeptides	1437:1449	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	8	62	theme	triplicate	1648:1657	arg1	analysis					1659:1666	the triplicate analysis	1644:1666	the triplicate analysis of 30 μg deglycosylated fetuin digest	1644:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	6	63	theme	serum	1251:1255	arg1	albumin					1257:1263	bovine serum albumin	1244:1263	bovine serum albumin trypsin digest as low as 1/500	1244:1294	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	1	64	theme	certain	178:184	arg1	pretreatments					186:198	certain pretreatments	178:198	certain pretreatments of samples	178:209	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	9	65	from	samples	1826:1832	arg1	glycoproteomics					1837:1851	glycoproteomics	1837:1851	glycoproteomics	1837:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	4	66	theme	mixture	874:880	arg1	digests					839:845	trypsin digests	831:845	trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins	831:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	9	67	theme	great	1790:1794	arg1	potential					1796:1804	great potential	1790:1804	great potential for pretreatment of samples in glycoproteomics	1790:1851	These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.					
30673210	4	68	theme	IgG	885:887	arg1	proteins					931:938	serum proteins	925:938	serum proteins	925:938	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	68	theme	IgG	885:887	arg1	mixture					874:880	mixture	874:880	mixture of IgG and bovine serum albumin (BSA)	874:918	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	68	theme	IgG	885:887	arg1	G					865:865	immunoglobulin G	850:865	immunoglobulin G (IgG)	850:871	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	68	theme	IgG	885:887	arg1	IgG					868:870	IgG	868:870	IgG	868:870	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	2	69	theme	hydrophilic	343:353	arg1	HILIC					383:387	HILIC	383:387	HILIC	383:387	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	2	69	theme	hydrophilic	343:353	arg1	chromatography					367:380	hydrophilic interaction chromatography	343:380	hydrophilic interaction chromatography (HILIC)	343:388	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	3	70	with	BHCM	680:683	arg1	macropores					717:726	honeycomb-like accessible macropores	691:726	honeycomb-like accessible macropores	691:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	7	71	from	proteins	1473:1480	arg1	sites					1417:1421	270 unique N-glycosylation sites	1390:1421	270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins	1390:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	71	from	proteins	1473:1480	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	2	72	theme	groups	605:610	arg1	modifications					563:575	modifications	563:575	modifications of high-density hydrophilic groups	563:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	7	73	theme	unique	1394:1399	arg1	sites					1417:1421	270 unique N-glycosylation sites	1390:1421	270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins	1390:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	7	73	theme	unique	1394:1399	arg1	glycopeptides					1437:1449	400 unique glycopeptides	1426:1449	400 unique glycopeptides from 146 glycosylated proteins	1426:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	6	74	theme	outstanding	1309:1319	arg1	specificity					1321:1331	outstanding specificity	1309:1331	outstanding specificity	1309:1331	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	3	75	theme	mm	631:632	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	75	theme	mm	631:632	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	2	76	theme	high-density	580:591	arg1	groups					605:610	high-density hydrophilic groups	580:610	high-density hydrophilic groups	580:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	6	77	theme	IgG	1222:1224	arg1	digest					1234:1239	IgG trypsin digest	1222:1239	IgG trypsin digest	1222:1239	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	5	78	theme	IgG	1103:1105	arg1	digest					1107:1112	the IgG digest	1099:1112	the IgG digest	1099:1112	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	6	79	dep	specificity	1321:1331	arg1	the					1305:1307	the	1305:1307	the	1305:1307	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	5	80	gly	glycopeptide	1180:1191	arg2	glycopeptide					1180:1191	glycopeptide	1180:1191	glycopeptide	1180:1191	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	3	81	theme	chitosan	661:668	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	81	theme	chitosan	661:668	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	82	theme	biomimetic	640:649	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	82	theme	biomimetic	640:649	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	7	83	from	sites	1417:1421	arg1	proteins					1473:1480	146 glycosylated proteins	1456:1480	146 glycosylated proteins	1456:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	6	84	theme	glycopeptide	1352:1363	arg1	enrichment					1365:1374	glycopeptide enrichment	1352:1374	glycopeptide enrichment	1352:1374	A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment.					
30673210	2	85	with	hindrance	520:528	arg1	modifications					563:575	modifications	563:575	modifications of high-density hydrophilic groups	563:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	2	86	theme	great	531:535	arg1	cost					537:540	great cost	531:540	great cost	531:540	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	0	87	theme	Peptides	112:119	arg1	Enrichment					85:94	Enrichment	85:94	Enrichment of Glycosylated Peptides	85:119	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	7	88	gly	glycosylated	1460:1471	arg1	proteins					1473:1480	146 glycosylated proteins	1456:1480	146 glycosylated proteins	1456:1480	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	2	89	theme	peptides	440:447	arg1	enrichment					413:422	enrichment	413:422	enrichment of glycosylated peptides	413:447	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	5	90	theme	high	1126:1129	arg1	fmol					1153:1156	about 50 fmol	1144:1156	about 50 fmol	1144:1156	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	5	90	theme	high	1126:1129	arg1	sensitivity					1131:1141	high sensitivity	1126:1141	high sensitivity (about 50 fmol)	1126:1157	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	2	91	with	difficulty	547:556	arg1	modifications					563:575	modifications	563:575	modifications of high-density hydrophilic groups	563:610	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	8	92	gly	O-glycosylation	1571:1585	arg2	sites					1587:1591	48 unique O-glycosylation sites	1561:1591	48 unique O-glycosylation sites of 278 unique O-glycopeptides	1561:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	92	gly	O-glycosylation	1571:1585	arg1	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	92	gly	O-glycosylation	1571:1585	arg2	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	92	gly	O-glycosylation	1571:1585	arg2	48					1561:1562	48	1561:1562	48	1561:1562	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	2	93	gly	glycosylated	427:438	arg1	peptides					440:447	glycosylated peptides	427:447	glycosylated peptides	427:447	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	5	94	from	digest	1107:1112	arg1	N-glycopeptides					1078:1092	32 N-glycopeptides	1075:1092	32 N-glycopeptides from the IgG digest	1075:1112	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	1	95	theme	glycopeptides	309:321	arg1	detection					323:331	glycopeptides detection	309:331	glycopeptides detection	309:331	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
30673210	2	96	theme	materials	476:484	arg1	plethora					452:459	a plethora	450:459	a plethora of hydrophilic materials	450:484	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	8	97	theme	unique	1564:1569	arg1	sites					1587:1591	48 unique O-glycosylation sites	1561:1591	48 unique O-glycosylation sites of 278 unique O-glycopeptides	1561:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	97	theme	unique	1564:1569	arg1	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	0	98	theme	Membrane	42:49	arg1	Fabrication					7:17	Facile Fabrication	0:17	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.	0:120	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	5	99	theme	glycopeptide	1180:1191	arg1	enrichment					1193:1202	glycopeptide enrichment	1180:1202	glycopeptide enrichment	1180:1202	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	5	100	theme	biomimetic	1029:1038	arg1	membrane					1040:1047	The biomimetic membrane	1025:1047	The biomimetic membrane	1025:1047	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	0	101	theme	Honeycomb-Like	56:69	arg1	Structure					71:79	Honeycomb-Like Structure	56:79	Honeycomb-Like Structure for Enrichment of Glycosylated Peptides	56:119	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	7	102	theme	human	1535:1539	arg1	serum					1541:1545	2 μL human serum	1530:1545	2 μL human serum	1530:1545	In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 μL human serum.					
30673210	2	103	theme	large	507:511	arg1	hindrance					520:528	large steric hindrance	507:528	large steric hindrance	507:528	Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups.					
30673210	0	104	with	Fabrication	7:17	arg1	Structure					71:79	Honeycomb-Like Structure	56:79	Honeycomb-Like Structure for Enrichment of Glycosylated Peptides	56:119	Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.					
30673210	3	105	theme	thick	634:638	arg1	adsorbent					785:793	adsorbent	785:793	adsorbent	785:793	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	3	105	theme	thick	634:638	arg1	BHCM					680:683	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM)	627:684	a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores	627:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	5	106	gly	N-glycopeptides	1078:1092	arg1	digest					1107:1112	the IgG digest	1099:1112	the IgG digest	1099:1112	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	5	106	gly	N-glycopeptides	1078:1092	arg2	N-glycopeptides					1078:1092	32 N-glycopeptides	1075:1092	32 N-glycopeptides from the IgG digest	1075:1112	The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment.					
30673210	8	107	theme	O-glycopeptides	1607:1621	arg1	sites					1587:1591	48 unique O-glycosylation sites	1561:1591	48 unique O-glycosylation sites of 278 unique O-glycopeptides	1561:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	8	107	theme	O-glycopeptides	1607:1621	arg1	O-glycopeptides					1607:1621	278 unique O-glycopeptides	1596:1621	278 unique O-glycopeptides	1596:1621	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	3	108	theme	honeycomb-like	691:704	arg1	macropores					717:726	honeycomb-like accessible macropores	691:726	honeycomb-like accessible macropores	691:726	In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC.					
30673210	8	109	theme	deglycosylated	1677:1690	arg1	digest					1699:1704	30 μg deglycosylated fetuin digest	1671:1704	30 μg deglycosylated fetuin digest	1671:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	4	110	theme	immunoglobulin	850:863	arg1	IgG					868:870	IgG	868:870	IgG	868:870	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	4	110	theme	immunoglobulin	850:863	arg1	G					865:865	immunoglobulin G	850:865	immunoglobulin G (IgG)	850:871	The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC.					
30673210	8	111	theme	digest	1699:1704	arg1	analysis					1659:1666	the triplicate analysis	1644:1666	the triplicate analysis of 30 μg deglycosylated fetuin digest	1644:1704	Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 μg deglycosylated fetuin digest.					
30673210	1	112	theme	nonglycopeptides	260:275	arg1	interference					244:255	the interference	240:255	the interference of nonglycopeptides	240:275	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection.					
29587227	3	0	theme	purified	715:722	arg1	sample					741:746	a purified mAb drug product sample	713:746	a purified mAb drug product sample	713:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	6	1	theme	associated	1323:1332	arg1	modifications					1353:1365	associated post-translational modifications	1323:1365	associated post-translational modifications	1323:1365	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	2	2	theme	low	389:391	arg1	LMW					411:413	LMW	411:413	LMW	411:413	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	2	2	theme	low	389:391	arg1	weight					403:408	low molecular weight	389:408	low molecular weight (LMW) impurities using those methods	389:445	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	7	3	theme	mass	1516:1519	arg1	analysis					1521:1528	intact mass analysis	1509:1528	intact mass analysis without prior enrichment	1509:1553	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	1	4	theme	monoclonal	310:319	arg1	mAb					331:333	mAb	331:333	mAb	331:333	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	1	4	theme	monoclonal	310:319	arg1	antibody					321:328	monoclonal antibody	310:328	therapeutic monoclonal antibody (mAb) drug products	298:348	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	4	5	theme	size-based	863:872	arg1	order					882:886	a size-based elution order	861:886	a size-based elution order	861:886	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	1	6	theme	modern	218:223	arg1	method					243:248	its modern equivalent CE-SDS method	214:248	its modern equivalent CE-SDS method	214:248	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	7	7	theme	product	1491:1497	arg1	sample					1499:1504	a mAb drug product sample	1480:1504	a mAb drug product sample	1480:1504	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	6	8	theme	protein	1187:1193	arg1	species					1214:1220	protein backbone-truncated species	1187:1220	protein backbone-truncated species	1187:1220	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	9	theme	mass	602:605	arg1	analysis					620:627	mass spectrometry analysis	602:627	mass spectrometry analysis	602:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	1	10	theme	CE-SDS	236:241	arg1	method					243:248	its modern equivalent CE-SDS method	214:248	its modern equivalent CE-SDS method	214:248	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	5	11	theme	mass	953:956	arg1	spectrometer					958:969	a high-resolution accurate mass spectrometer	926:969	a high-resolution accurate mass spectrometer	926:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	0	12	theme	therapeutic	71:81	arg1	antibodies					94:103	therapeutic monoclonal antibodies	71:103	therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry	71:179	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	1	13	theme	SDS-PAGE	194:201	arg1	method					203:208	Traditional SDS-PAGE method	182:208	Traditional SDS-PAGE method	182:208	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	5	14	theme	high-resolution	928:942	arg1	spectrometer					958:969	a high-resolution accurate mass spectrometer	926:969	a high-resolution accurate mass spectrometer	926:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	4	15	theme	N-linked	784:791	arg1	glycans					793:799	N-linked glycans	784:799	N-linked glycans	784:799	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	0	16	theme	hydrophilic	111:121	arg1	chromatography					135:148	hydrophilic interaction chromatography	111:148	hydrophilic interaction chromatography coupled with mass spectrometry	111:179	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	5	17	from	spectrometer	958:969	arg1	measurement					909:919	The subsequent mass measurement	889:919	The subsequent mass measurement from a high-resolution accurate mass spectrometer	889:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	1	18	theme	antibody	321:328	arg1	products					341:348	therapeutic monoclonal antibody (mAb) drug products	298:348	therapeutic monoclonal antibody (mAb) drug products	298:348	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	4	19	theme	LMW	841:843	arg1	impurities					845:854	mAb-related LMW impurities	829:854	mAb-related LMW impurities	829:854	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	0	20	theme	product-related	20:34	arg1	weight					50:55	product-related low molecular weight	20:55	product-related low molecular weight	20:55	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	6	21	theme	light	1100:1104	arg1	chain					1106:1110	Free light chain	1095:1110	Free light chain	1095:1110	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	6	21	theme	light	1100:1104	arg1	antibody					1118:1125	half antibody	1113:1125	half antibody	1113:1125	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	22	theme	LMW	683:685	arg1	impurities					687:696	such LMW impurities	678:696	such LMW impurities present within a purified mAb drug product sample	678:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	6	23	dep	species	1132:1138	arg1	antibody					1141:1148	antibody	1141:1148	antibody possessing a single light chain	1141:1180	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	0	24	theme	molecular	40:48	arg1	weight					50:55	product-related low molecular weight	20:55	product-related low molecular weight	20:55	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	1	25	theme	drug	336:339	arg1	products					341:348	therapeutic monoclonal antibody (mAb) drug products	298:348	therapeutic monoclonal antibody (mAb) drug products	298:348	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	5	26	theme	single	1072:1077	arg1	analysis					1085:1092	a single LC-MS analysis	1070:1092	a single LC-MS analysis	1070:1092	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	2	27	theme	empirical	489:497	arg1	knowledges					499:508	empirical knowledges	489:508	empirical knowledges	489:508	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	3	28	attach	present	529:535	arg1	paper					519:523	this paper	514:523	this paper	514:523	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	28	attach	present	529:535	arg2	we					526:527	we	526:527	we	526:527	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	6	29	theme	single	1163:1168	arg1	chain					1176:1180	a single light chain	1161:1180	a single light chain	1161:1180	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	4	30	theme	HILIC	806:810	arg1	method					812:817	the HILIC method	802:817	the HILIC method	802:817	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	2	31	theme	structural	360:369	arg1	identification					371:384	structural identification	360:384	structural identification of low molecular weight (LMW) impurities using those methods	360:445	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	7	32	theme	prior	1538:1542	arg1	enrichment					1544:1553	prior enrichment	1538:1553	prior enrichment	1538:1553	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	5	33	theme	LMW	1048:1050	arg1	impurities					1052:1061	low-abundance LMW impurities	1034:1061	low-abundance LMW impurities	1034:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	3	34	theme	present	698:704	arg1	impurities					687:696	such LMW impurities	678:696	such LMW impurities present within a purified mAb drug product sample	678:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	35	theme	product	733:739	arg1	sample					741:746	a purified mAb drug product sample	713:746	a purified mAb drug product sample	713:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	6	36	theme	post-translational	1334:1351	arg1	modifications					1353:1365	associated post-translational modifications	1323:1365	associated post-translational modifications	1323:1365	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	2	37	theme	molecular	393:401	arg1	LMW					411:413	LMW	411:413	LMW	411:413	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	2	37	theme	molecular	393:401	arg1	weight					403:408	low molecular weight	389:408	low molecular weight (LMW) impurities using those methods	389:445	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	7	38	theme	intact	1509:1514	arg1	analysis					1521:1528	intact mass analysis	1509:1528	intact mass analysis without prior enrichment	1509:1553	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	6	39	theme	H2L	1128:1130	arg1	species					1132:1138	Free light chain, half antibody, H2L species	1095:1138	Free light chain, half antibody, H2L species (antibody possessing a single light chain)	1095:1181	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	40	theme	mAb	724:726	arg1	sample					741:746	a purified mAb drug product sample	713:746	a purified mAb drug product sample	713:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	1	41	theme	therapeutic	298:308	arg1	products					341:348	therapeutic monoclonal antibody (mAb) drug products	298:348	therapeutic monoclonal antibody (mAb) drug products	298:348	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	7	42	theme	mAb	1482:1484	arg1	sample					1499:1504	a mAb drug product sample	1480:1504	a mAb drug product sample	1480:1504	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	6	43	theme	truncation	1302:1311	arg1	sites					1313:1317	the truncation sites	1298:1317	the truncation sites	1298:1317	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	44	theme	interaction	554:564	arg1	HILIC					582:586	HILIC	582:586	HILIC	582:586	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	44	theme	interaction	554:564	arg1	chromatography					566:579	hydrophilic interaction chromatography	542:579	hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis	542:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	44	theme	interaction	554:564	arg1	method					655:660	a novel and orthogonal method	632:660	a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample	632:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	1	45	theme	equivalent	225:234	arg1	method					243:248	its modern equivalent CE-SDS method	214:248	its modern equivalent CE-SDS method	214:248	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	7	46	theme	drug	1486:1489	arg1	sample					1499:1504	a mAb drug product sample	1480:1504	a mAb drug product sample	1480:1504	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	6	47	theme	backbone-truncated	1195:1212	arg1	species					1214:1220	protein backbone-truncated species	1187:1220	protein backbone-truncated species	1187:1220	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	5	48	theme	subsequent	893:902	arg1	measurement					909:919	The subsequent mass measurement	889:919	The subsequent mass measurement from a high-resolution accurate mass spectrometer	889:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	4	49	theme	elution	874:880	arg1	order					882:886	a size-based elution order	861:886	a size-based elution order	861:886	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	0	50	theme	mass	163:166	arg1	spectrometry					168:179	mass spectrometry	163:179	mass spectrometry	163:179	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	6	51	contain	possessing	1150:1159	arg2	chain					1176:1180	a single light chain	1161:1180	a single light chain	1161:1180	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	6	51	contain	possessing	1150:1159	arg1	antibody					1141:1148	antibody	1141:1148	antibody possessing a single light chain	1141:1180	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	5	52	theme	mass	904:907	arg1	measurement					909:919	The subsequent mass measurement	889:919	The subsequent mass measurement from a high-resolution accurate mass spectrometer	889:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	1	53	dep	applied	266:272	arg1	both					254:257	both	254:257	both	254:257	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	6	54	theme	great	1274:1278	arg1	detail					1280:1285	great detail	1274:1285	great detail	1274:1285	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	55	theme	spectrometry	607:618	arg1	analysis					620:627	mass spectrometry analysis	602:627	mass spectrometry analysis	602:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	5	56	theme	accurate	944:951	arg1	spectrometer					958:969	a high-resolution accurate mass spectrometer	926:969	a high-resolution accurate mass spectrometer	926:969	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	3	57	theme	orthogonal	644:653	arg1	chromatography					566:579	hydrophilic interaction chromatography	542:579	hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis	542:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	57	theme	orthogonal	644:653	arg1	method					655:660	a novel and orthogonal method	632:660	a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample	632:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	7	58	theme	first	1416:1420	arg1	example					1422:1428	the first example	1412:1428	the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment	1412:1553	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	0	59	theme	monoclonal	83:92	arg1	antibodies					94:103	therapeutic monoclonal antibodies	71:103	therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry	71:179	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	3	60	theme	novel	634:638	arg1	chromatography					566:579	hydrophilic interaction chromatography	542:579	hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis	542:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	60	theme	novel	634:638	arg1	method					655:660	a novel and orthogonal method	632:660	a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample	632:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	1	61	theme	Traditional	182:192	arg1	method					203:208	Traditional SDS-PAGE method	182:208	Traditional SDS-PAGE method	182:208	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	0	62	theme	interaction	123:133	arg1	chromatography					135:148	hydrophilic interaction chromatography	111:148	hydrophilic interaction chromatography coupled with mass spectrometry	111:179	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	5	63	theme	variety	1023:1029	arg1	identification					1003:1016	direct and unambiguous identification	980:1016	direct and unambiguous identification of a variety of low-abundance LMW impurities	980:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	4	64	theme	glycans	793:799	arg1	removal					773:779	removal	773:779	removal of N-linked glycans	773:799	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	2	65	theme	impurities	416:425	arg1	identification					371:384	structural identification	360:384	structural identification of low molecular weight (LMW) impurities using those methods	360:445	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	5	66	theme	direct	980:985	arg1	identification					1003:1016	direct and unambiguous identification	980:1016	direct and unambiguous identification of a variety of low-abundance LMW impurities	980:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	6	67	theme	half	1113:1116	arg1	chain					1106:1110	Free light chain	1095:1110	Free light chain	1095:1110	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	6	67	theme	half	1113:1116	arg1	antibody					1118:1125	half antibody	1113:1125	half antibody	1113:1125	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	0	68	theme	low	36:38	arg1	weight					50:55	product-related low molecular weight	20:55	product-related low molecular weight	20:55	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	5	69	theme	unambiguous	991:1001	arg1	identification					1003:1016	direct and unambiguous identification	980:1016	direct and unambiguous identification of a variety of low-abundance LMW impurities	980:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	6	70	theme	chain	1106:1110	arg1	species					1132:1138	Free light chain, half antibody, H2L species	1095:1138	Free light chain, half antibody, H2L species (antibody possessing a single light chain)	1095:1181	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	3	71	theme	such	678:681	arg1	impurities					687:696	such LMW impurities	678:696	such LMW impurities present within a purified mAb drug product sample	678:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	0	72	theme	weight	50:55	arg1	Characterization					0:15	Characterization	0:15	Characterization of product-related low molecular weight	0:55	Characterization of product-related low molecular weight impurities in therapeutic monoclonal antibodies using hydrophilic interaction chromatography coupled with mass spectrometry.					
29587227	6	73	theme	Free	1095:1098	arg1	chain					1106:1110	Free light chain	1095:1110	Free light chain	1095:1110	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	6	73	theme	Free	1095:1098	arg1	antibody					1118:1125	half antibody	1113:1125	half antibody	1113:1125	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	4	74	theme	mAb-related	829:839	arg1	impurities					845:854	mAb-related LMW impurities	829:854	mAb-related LMW impurities	829:854	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	1	75	theme	products	341:348	arg1	purity					288:293	the purity	284:293	the purity of therapeutic monoclonal antibody (mAb) drug products	284:348	Traditional SDS-PAGE method and its modern equivalent CE-SDS method are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products.					
29587227	5	76	theme	LC-MS	1079:1083	arg1	analysis					1085:1092	a single LC-MS analysis	1070:1092	a single LC-MS analysis	1070:1092	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	6	77	theme	light	1170:1174	arg1	chain					1176:1180	a single light chain	1161:1180	a single light chain	1161:1180	Free light chain, half antibody, H2L species (antibody possessing a single light chain) and protein backbone-truncated species can all be confidently identified and elucidated in great detail, including the truncation sites and associated post-translational modifications.					
29587227	7	78	located	detected	1468:1475	arg1	sample					1499:1504	a mAb drug product sample	1480:1504	a mAb drug product sample	1480:1504	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	7	78	located	detected	1468:1475	arg2	species					1444:1450	the H2L species	1436:1450	the H2L species	1436:1450	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	5	79	theme	low-abundance	1034:1046	arg1	impurities					1052:1061	low-abundance LMW impurities	1034:1061	low-abundance LMW impurities	1034:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	7	80	theme	H2L	1440:1442	arg1	species					1444:1450	the H2L species	1436:1450	the H2L species	1436:1450	It is worth noting that this study provides the first example where the H2L species can be directly detected in a mAb drug product sample by intact mass analysis without prior enrichment.					
29587227	4	81	link	N-linked	784:791	arg1	glycans					793:799	N-linked glycans	784:799	N-linked glycans	784:799	We show here that after removal of N-linked glycans, the HILIC method separates mAb-related LMW impurities with a size-based elution order.					
29587227	2	82	theme	weight	403:408	arg1	impurities					416:425	low molecular weight (LMW) impurities	389:425	low molecular weight (LMW) impurities using those methods	389:445	However, structural identification of low molecular weight (LMW) impurities using those methods has been challenging and largely based on empirical knowledges.					
29587227	5	83	theme	impurities	1052:1061	arg1	impurities					1052:1061	low-abundance LMW impurities	1034:1061	low-abundance LMW impurities	1034:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	5	83	theme	impurities	1052:1061	arg1	variety					1023:1029	a variety	1021:1029	a variety of low-abundance LMW impurities	1021:1061	The subsequent mass measurement from a high-resolution accurate mass spectrometer provides direct and unambiguous identification of a variety of low-abundance LMW impurities within a single LC-MS analysis.					
29587227	3	84	theme	drug	728:731	arg1	sample					741:746	a purified mAb drug product sample	713:746	a purified mAb drug product sample	713:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	85	theme	hydrophilic	542:552	arg1	HILIC					582:586	HILIC	582:586	HILIC	582:586	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	85	theme	hydrophilic	542:552	arg1	chromatography					566:579	hydrophilic interaction chromatography	542:579	hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis	542:627	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
29587227	3	85	theme	hydrophilic	542:552	arg1	method					655:660	a novel and orthogonal method	632:660	a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample	632:746	In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel and orthogonal method to characterize such LMW impurities present within a purified mAb drug product sample.					
31506992	0	0	theme	Streptococcus	77:89	arg1	Glycans					99:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	1	1	theme	vaccine	267:273	arg1	development					275:285	vaccine development	267:285	vaccine development	267:285	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	0	2	theme	Ib	74:75	arg1	Glycans					99:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	1	3	theme	Group	180:184	arg1	serotypes					202:210	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	3	theme	Group	180:184	arg1	Ib					219:220	Ib	219:220	Ib	219:220	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	3	theme	Group	180:184	arg1	Ia					212:213	Ia	212:213	Ia	212:213	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	3	theme	Group	180:184	arg1	targets					255:261	key targets	251:261	key targets for vaccine development	251:285	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	0	4	theme	Glycans	99:105	arg1	Synthesis					48:56	the Synthesis	44:56	the Synthesis of Group Ia and Ib Streptococcus Related Glycans	44:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	5	5	theme	polymer	1035:1041	arg1	conformation					827:838	conformation	827:838	conformation	827:838	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	5	theme	polymer	1035:1041	arg1	dynamics					854:861	molecular dynamics	844:861	molecular dynamics	844:861	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	5	theme	polymer	1035:1041	arg1	flexibility					1017:1027	a higher flexibility	1008:1027	a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure	1008:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	6	theme	moieties	963:970	arg1	presentation					918:929	the different presentation	904:929	the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones	904:1002	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	0	7	theme	Related	91:97	arg1	Glycans					99:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	4	8	theme	B	730:730	arg1	GBS					747:749	different group B Streptococcus (GBS)	714:750	different group B Streptococcus (GBS)	714:750	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	4	9	theme	group	724:728	arg1	GBS					747:749	different group B Streptococcus (GBS)	714:750	different group B Streptococcus (GBS)	714:750	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	5	10	theme	protruding	938:947	arg1	moieties					963:970	the protruding Neu5Acα2-3Gal moieties	934:970	the protruding Neu5Acα2-3Gal moieties	934:970	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	11	from	linkage	534:540	arg1	Ia					554:555	serotype Ia	545:555	serotype Ia	545:555	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	3	11	from	linkage	534:540	arg1	Ib					588:589	a β1-3 linkage in serotype Ib	561:589	a β1-3 linkage in serotype Ib	561:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	5	12	theme	polysaccharide	979:992	arg1	backbones					994:1002	the polysaccharide backbones	975:1002	the polysaccharide backbones	975:1002	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	1	13	theme	B	186:186	arg1	serotypes					202:210	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	13	theme	B	186:186	arg1	Ib					219:220	Ib	219:220	Ib	219:220	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	13	theme	B	186:186	arg1	Ia					212:213	Ia	212:213	Ia	212:213	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	13	theme	B	186:186	arg1	targets					255:261	key targets	251:261	key targets for vaccine development	251:285	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	5	14	from	presentation	918:929	arg1	backbones					994:1002	the polysaccharide backbones	975:1002	the polysaccharide backbones	975:1002	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	15	theme	serotype	545:552	arg1	Ia					554:555	serotype Ia	545:555	serotype Ia	545:555	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	1	16	theme	Streptococcus	188:200	arg1	serotypes					202:210	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	16	theme	Streptococcus	188:200	arg1	Ib					219:220	Ib	219:220	Ib	219:220	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	16	theme	Streptococcus	188:200	arg1	Ia					212:213	Ia	212:213	Ia	212:213	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	16	theme	Streptococcus	188:200	arg1	targets					255:261	key targets	251:261	key targets for vaccine development	251:285	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	0	17	theme	Glycosylation	15:27	arg1	Strategies					29:38	Regioselective Glycosylation Strategies	0:38	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans	0:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	4	18	theme	Gal	666:668	arg1	synthons					670:677	GlcNAcβ1-3[Glcβ1-4]Gal synthons	647:677	GlcNAcβ1-3[Glcβ1-4]Gal synthons	647:677	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	2	19	theme	high	350:353	arg1	similarity					366:375	high structural similarity	350:375	high structural similarity	350:375	In spite of their immunospecifity these polysaccharides share high structural similarity.					
31506992	5	20	used	used	809:812	arg2	glycans					796:802	These glycans	790:802	These glycans	790:802	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	0	21	theme	Regioselective	0:13	arg1	Strategies					29:38	Regioselective Glycosylation Strategies	0:38	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans	0:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	3	22	theme	in	576:577	arg1	Ib					588:589	a β1-3 linkage in serotype Ib	561:589	a β1-3 linkage in serotype Ib	561:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	3	23	theme	same	403:406	arg1	residues					423:430	the same monosaccharide residues	399:430	the same monosaccharide residues	399:430	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	4	24	theme	Streptococcus	732:744	arg1	GBS					747:749	different group B Streptococcus (GBS)	714:750	different group B Streptococcus (GBS)	714:750	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	5	25	theme	Neu5Acα2-3Gal	949:961	arg1	moieties					963:970	the protruding Neu5Acα2-3Gal moieties	934:970	the protruding Neu5Acα2-3Gal moieties	934:970	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	26	theme	monosaccharide	408:421	arg1	residues					423:430	the same monosaccharide residues	399:430	the same monosaccharide residues	399:430	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	0	27	theme	Unique	126:131	arg1	Conformations					133:145	Unique Conformations	126:145	Unique Conformations of the Capsular Polysaccharides	126:177	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	3	28	theme	β1-3	563:566	arg1	Ib					588:589	a β1-3 linkage in serotype Ib	561:589	a β1-3 linkage in serotype Ib	561:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	4	29	theme	routes	636:641	arg1	development					596:606	The development	592:606	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons	592:677	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	3	30	theme	side	487:490	arg1	chain					492:496	the Neu5Acα2-3Gal side chain	469:496	the Neu5Acα2-3Gal side chain	469:496	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	1	31	dep	serotypes	202:210	arg1	serotypes					202:210	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	31	dep	serotypes	202:210	arg1	Ib					219:220	Ib	219:220	Ib	219:220	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	31	dep	serotypes	202:210	arg1	Ia					212:213	Ia	212:213	Ia	212:213	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	32	theme	capsular	222:229	arg1	polysaccharides					231:245	capsular polysaccharides	222:245	capsular polysaccharides	222:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	5	33	theme	molecular	844:852	arg1	dynamics					854:861	molecular dynamics	844:861	molecular dynamics	844:861	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	34	theme	epitope	1076:1082	arg1	exposure					1084:1091	epitope exposure	1076:1091	epitope exposure	1076:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	1	35	dep	Ia	212:213	arg1	polysaccharides					231:245	capsular polysaccharides	222:245	capsular polysaccharides	222:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	3	36	theme	chain	492:496	arg1	connection					455:464	the connection	451:464	the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib	451:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	0	37	theme	Group	61:65	arg1	Ia					67:68	Group Ia	61:68	Group Ia	61:68	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	0	38	theme	Polysaccharides	163:177	arg1	Conformations					133:145	Unique Conformations	126:145	Unique Conformations of the Capsular Polysaccharides	126:177	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	3	39	theme	serotype	579:586	arg1	Ib					588:589	a β1-3 linkage in serotype Ib	561:589	a β1-3 linkage in serotype Ib	561:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	4	40	theme	different	714:722	arg1	GBS					747:749	different group B Streptococcus (GBS)	714:750	different group B Streptococcus (GBS)	714:750	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	0	41	theme	Capsular	154:161	arg1	Polysaccharides					163:177	the Capsular Polysaccharides	150:177	the Capsular Polysaccharides	150:177	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	5	42	theme	relative	1043:1050	arg1	polymer					1035:1041	Ib polymer	1032:1041	Ib polymer relative to Ia, which can impact epitope exposure	1032:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	43	theme	different	908:916	arg1	presentation					918:929	the different presentation	904:929	the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones	904:1002	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	44	theme	linkage	568:574	arg1	Ib					588:589	a β1-3 linkage in serotype Ib	561:589	a β1-3 linkage in serotype Ib	561:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	5	45	dep	conformation	827:838	arg1	the					823:825	the	823:825	the	823:825	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	46	theme	Ib	1032:1033	arg1	polymer					1035:1041	Ib polymer	1032:1041	Ib polymer relative to Ia, which can impact epitope exposure	1032:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	47	theme	GlcNAc	505:510	arg1	unit					512:515	the GlcNAc unit	501:515	the GlcNAc unit	501:515	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	3	47	theme	GlcNAc	505:510	arg1	linkage					534:540	a β1-4 linkage	527:540	a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib	527:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	4	48	theme	unit	772:775	arg1	frameshifts					777:787	unit frameshifts	772:787	unit frameshifts	772:787	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	2	49	theme	structural	355:364	arg1	similarity					366:375	high structural similarity	350:375	high structural similarity	350:375	In spite of their immunospecifity these polysaccharides share high structural similarity.					
31506992	1	50	theme	key	251:253	arg1	targets					255:261	key targets	251:261	key targets for vaccine development	251:285	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	1	50	theme	key	251:253	arg1	serotypes					202:210	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides	180:245	Group B Streptococcus serotypes Ia and Ib capsular polysaccharides are key targets for vaccine development.					
31506992	5	51	theme	higher	1010:1015	arg1	flexibility					1017:1027	a higher flexibility	1008:1027	a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure	1008:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	3	52	theme	β1-4	529:532	arg1	unit					512:515	the GlcNAc unit	501:515	the GlcNAc unit	501:515	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	3	52	theme	β1-4	529:532	arg1	linkage					534:540	a β1-4 linkage	527:540	a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib	527:589	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	3	53	theme	Neu5Acα2-3Gal	473:485	arg1	chain					492:496	the Neu5Acα2-3Gal side chain	469:496	the Neu5Acα2-3Gal side chain	469:496	Both are composed of the same monosaccharide residues and differ only in the connection of the Neu5Acα2-3Gal side chain to the GlcNAc unit, which is a β1-4 linkage in serotype Ia and a β1-3 linkage in serotype Ib.					
31506992	4	54	theme	regioselective	621:634	arg1	routes					636:641	efficient regioselective routes	611:641	efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons	611:677	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31506992	0	55	theme	Ia	67:68	arg1	Glycans					99:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Group Ia and Ib Streptococcus Related Glycans	61:105	Regioselective Glycosylation Strategies for the Synthesis of Group Ia and Ib Streptococcus Related Glycans Enable Elucidating Unique Conformations of the Capsular Polysaccharides.					
31506992	5	56	theme	polysaccharides	874:888	arg1	conformation					827:838	conformation	827:838	conformation	827:838	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	56	theme	polysaccharides	874:888	arg1	dynamics					854:861	molecular dynamics	844:861	molecular dynamics	844:861	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	5	56	theme	polysaccharides	874:888	arg1	flexibility					1017:1027	a higher flexibility	1008:1027	a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure	1008:1091	These glycans were used to probe the conformation and molecular dynamics of the two polysaccharides, highlighting the different presentation of the protruding Neu5Acα2-3Gal moieties on the polysaccharide backbones and a higher flexibility of Ib polymer relative to Ia, which can impact epitope exposure.					
31506992	4	57	theme	efficient	611:619	arg1	routes					636:641	efficient regioselective routes	611:641	efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons	611:677	The development of efficient regioselective routes for GlcNAcβ1-3[Glcβ1-4]Gal synthons is described, which give access to different group B Streptococcus (GBS) Ia and Ib repeating unit frameshifts.					
31021647	3	0	theme	magnitude	497:505	arg1	order					488:492	an order	485:492	an order of magnitude	485:505	Through this approach, the hexasaccharide was obtained in 28 steps and 8.9% overall yield, which is an order of magnitude higher than that of previously reported approaches.					
31021647	0	1	theme	Mild	65:68	arg1	Approach					90:97	a Mild, Reagent-Controlled Approach	63:97	a Mild, Reagent-Controlled Approach	63:97	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	1	2	theme	A	159:159	arg1	fragment					136:143	the hexasaccharide fragment	117:143	the hexasaccharide fragment of landomycin A	117:159	The synthesis of the hexasaccharide fragment of landomycin A is reported.					
31021647	3	3	theme	reported	538:545	arg1	approaches					547:556	previously reported approaches	527:556	previously reported approaches	527:556	Through this approach, the hexasaccharide was obtained in 28 steps and 8.9% overall yield, which is an order of magnitude higher than that of previously reported approaches.					
31021647	1	4	theme	landomycin	148:157	arg1	A					159:159	landomycin A	148:159	landomycin A	148:159	The synthesis of the hexasaccharide fragment of landomycin A is reported.					
31021647	1	5	theme	hexasaccharide	121:134	arg1	fragment					136:143	the hexasaccharide fragment	117:143	the hexasaccharide fragment of landomycin A	117:159	The synthesis of the hexasaccharide fragment of landomycin A is reported.					
31021647	2	6	theme	stereoselective	288:302	arg1	fashion					304:310	a stereoselective fashion	286:310	a stereoselective fashion	286:310	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	3	7	theme	8.9	456:458	arg1	%					459:459	%	459:459	%	459:459	Through this approach, the hexasaccharide was obtained in 28 steps and 8.9% overall yield, which is an order of magnitude higher than that of previously reported approaches.					
31021647	3	8	theme	%	459:459	arg1	yield					469:473	8.9% overall yield	456:473	8.9% overall yield	456:473	Through this approach, the hexasaccharide was obtained in 28 steps and 8.9% overall yield, which is an order of magnitude higher than that of previously reported approaches.					
31021647	2	9	theme	temporary	333:341	arg1	groups					354:359	temporary prosthetic groups	333:359	temporary prosthetic groups	333:359	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	0	10	dep	Mild	65:68	arg1	Reagent-Controlled					71:88	Reagent-Controlled	71:88	Reagent-Controlled	71:88	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	0	11	theme	Hexasaccharide	17:30	arg1	Fragment					32:39	the Hexasaccharide Fragment	13:39	the Hexasaccharide Fragment of Landomycin A	13:55	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	2	12	from	linkages	274:281	arg1	fashion					304:310	a stereoselective fashion	286:310	a stereoselective fashion	286:310	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	3	13	theme	overall	461:467	arg1	yield					469:473	8.9% overall yield	456:473	8.9% overall yield	456:473	Through this approach, the hexasaccharide was obtained in 28 steps and 8.9% overall yield, which is an order of magnitude higher than that of previously reported approaches.					
31021647	0	14	theme	Fragment	32:39	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the Hexasaccharide Fragment of Landomycin A	0:55	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	0	15	theme	A	55:55	arg1	Fragment					32:39	the Hexasaccharide Fragment	13:39	the Hexasaccharide Fragment of Landomycin A	13:55	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	0	16	theme	Landomycin	44:53	arg1	A					55:55	Landomycin A	44:55	Landomycin A	44:55	Synthesis of the Hexasaccharide Fragment of Landomycin A Using a Mild, Reagent-Controlled Approach.					
31021647	2	17	theme	dehydrative	216:226	arg1	glycosylation					228:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	2	18	theme	mediated	207:214	arg1	glycosylation					228:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	2	19	theme	chloride	198:205	arg1	glycosylation					228:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	2	20	theme	prosthetic	343:352	arg1	groups					354:359	temporary prosthetic groups	333:359	temporary prosthetic groups	333:359	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	2	21	theme	p-toluenesulfonyl	180:196	arg1	glycosylation					228:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	p-toluenesulfonyl chloride mediated dehydrative glycosylation	180:240	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	2	22	theme	deoxy-sugar	262:272	arg1	linkages					274:281	the deoxy-sugar linkages	258:281	the deoxy-sugar linkages in a stereoselective fashion	258:310	Using p-toluenesulfonyl chloride mediated dehydrative glycosylation, we constructed the deoxy-sugar linkages in a stereoselective fashion without the need for temporary prosthetic groups to control selectivity.					
31021647	1	23	theme	fragment	136:143	arg1	synthesis					104:112	The synthesis	100:112	The synthesis of the hexasaccharide fragment of landomycin A	100:159	The synthesis of the hexasaccharide fragment of landomycin A is reported.					
30406451	0	0	theme	selected	80:87	arg1	bacteria					93:100	selected gut bacteria	80:100	selected gut bacteria	80:100	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	1	1	with	derivatives	170:180	arg1	products					242:249	products	242:249	products of Lactobacillus reuteri glucansucrases	242:289	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	1	with	derivatives	170:180	arg1	sucrose					309:315	sucrose	309:315	sucrose	309:315	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	1	with	derivatives	170:180	arg1	degree					197:202	a degree	195:202	a degree of polymerization (DP) of 3-4 (GL34)	195:239	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	1	with	derivatives	170:180	arg1	lactose					297:303	lactose	297:303	lactose	297:303	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	5	2	theme	Escherichia	752:762	arg1	bacteria					743:750	the commensal bacteria	729:750	the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron	729:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	2	theme	Escherichia	752:762	arg1	Nissle					769:774	Escherichia coli Nissle	752:774	Escherichia coli Nissle	752:774	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	4	3	theme	GL34	657:660	arg1	mixture					662:668	the GL34 mixture	653:668	the GL34 mixture	653:668	Bifidobacteria clearly grew better on the GL34 mixture than lactobacilli and commensal bacteria.					
30406451	0	4	theme	bacteria	93:100	arg1	growth					70:75	growth	70:75	growth of selected gut bacteria	70:100	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	3	5	theme	bacteria	508:515	arg1	strains					493:499	single strains	486:499	single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria,	486:570	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	0	6	theme	gut	89:91	arg1	bacteria					93:100	selected gut bacteria	80:100	selected gut bacteria	80:100	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	8	7	theme	cheap	1303:1307	arg1	lactose					1334:1340	cheap and abundantly available lactose	1303:1340	cheap and abundantly available lactose	1303:1340	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	0	8	theme	Stimulatory	0:10	arg1	effects					12:18	Stimulatory effects	0:18	Stimulatory effects of novel glucosylated lactose derivatives	0:60	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	5	9	theme	compound	830:837	arg1	1 → 4					867:871	1 → 4	867:871	1 → 4	867:871	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	9	theme	compound	830:837	arg1	-[β-D-Galp-					855:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-	823:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp	823:880	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	8	10	theme	available	1324:1332	arg1	lactose					1334:1340	cheap and abundantly available lactose	1303:1340	cheap and abundantly available lactose	1303:1340	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	5	11	theme	1 → 2	849:853	arg1	1 → 4					867:871	1 → 4	867:871	1 → 4	867:871	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	11	theme	1 → 2	849:853	arg1	-[β-D-Galp-					855:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-	823:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp	823:880	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	12	theme	Bacteroides	780:790	arg1	bacteria					743:750	the commensal bacteria	729:750	the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron	729:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	12	theme	Bacteroides	780:790	arg1	thetaiotaomicron					792:807	Bacteroides thetaiotaomicron	780:807	Bacteroides thetaiotaomicron	780:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	0	13	theme	lactose	42:48	arg1	derivatives					50:60	novel glucosylated lactose derivatives	23:60	novel glucosylated lactose derivatives	23:60	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	3	14	theme	gut	504:506	arg1	bacteria					508:515	gut bacteria	504:515	gut bacteria	504:515	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	7	15	theme	Bifidobacterium	1031:1045	arg1	adolescentis					1047:1058	Bifidobacterium adolescentis	1031:1058	Bifidobacterium adolescentis	1031:1058	Bifidobacterium adolescentis was most effective, completely degrading four of the five GL34 compounds, leaving only one minor constituent.					
30406451	3	16	theme	lactobacilli	534:545	arg1	strains					493:499	single strains	486:499	single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria,	486:570	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	2	17	theme	hydrolytic	401:410	arg1	activities					412:421	the hydrolytic activities	397:421	the hydrolytic activities of common carbohydrate-degrading enzymes	397:462	Here, we show that these GL34 compounds are largely resistant to the hydrolytic activities of common carbohydrate-degrading enzymes.					
30406451	8	18	theme	novel	1193:1197	arg1	mixture					1215:1221	a novel oligosaccharide mixture	1191:1221	a novel oligosaccharide mixture	1191:1221	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	6	19	theme	specific	1007:1014	arg1	strain					1016:1021	the specific strain	1003:1021	the specific strain tested	1003:1028	Bifidobacteria digested more than one compound from the GL34 mixture, varying with the specific strain tested.					
30406451	0	20	theme	novel	23:27	arg1	derivatives					50:60	novel glucosylated lactose derivatives	23:60	novel glucosylated lactose derivatives	23:60	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	5	21	dep	bacteria	743:750	arg1	bacteria					743:750	the commensal bacteria	729:750	the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron	729:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	21	dep	bacteria	743:750	arg1	Nissle					769:774	Escherichia coli Nissle	752:774	Escherichia coli Nissle	752:774	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	21	dep	bacteria	743:750	arg1	thetaiotaomicron					792:807	Bacteroides thetaiotaomicron	780:807	Bacteroides thetaiotaomicron	780:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	3	22	theme	bifidobacteria	518:531	arg1	strains					493:499	single strains	486:499	single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria,	486:570	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	5	23	dep	-[β-D-Galp-	855:865	arg1	D-Glcp					875:880	D-Glcp	875:880	D-Glcp	875:880	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	24	theme	F2	827:828	arg1	1 → 4					867:871	1 → 4	867:871	1 → 4	867:871	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	24	theme	F2	827:828	arg1	-[β-D-Galp-					855:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-	823:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp	823:880	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	7	25	theme	compounds	1123:1131	arg1	compounds					1123:1131	the five GL34 compounds	1109:1131	the five GL34 compounds	1109:1131	Bifidobacterium adolescentis was most effective, completely degrading four of the five GL34 compounds, leaving only one minor constituent.					
30406451	7	25	theme	compounds	1123:1131	arg1	four					1101:1104	four	1101:1104	four	1101:1104	Bifidobacterium adolescentis was most effective, completely degrading four of the five GL34 compounds, leaving only one minor constituent.					
30406451	1	26	theme	Lactobacillus	254:266	arg1	glucansucrases					276:289	Lactobacillus reuteri glucansucrases	254:289	Lactobacillus reuteri glucansucrases	254:289	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	5	27	dep	Escherichia	752:762	arg1	coli					764:767	coli	764:767	coli	764:767	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	3	28	theme	single	486:491	arg1	strains					493:499	single strains	486:499	single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria,	486:570	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	2	29	theme	enzymes	456:462	arg1	activities					412:421	the hydrolytic activities	397:421	the hydrolytic activities of common carbohydrate-degrading enzymes	397:462	Here, we show that these GL34 compounds are largely resistant to the hydrolytic activities of common carbohydrate-degrading enzymes.					
30406451	5	30	theme	α-D-Glcp-	839:847	arg1	1 → 4					867:871	1 → 4	867:871	1 → 4	867:871	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	30	theme	α-D-Glcp-	839:847	arg1	-[β-D-Galp-					855:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-	823:865	the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp	823:880	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	3	31	theme	bacteria	562:569	arg1	strains					493:499	single strains	486:499	single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria,	486:570	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	8	32	theme	oligosaccharide	1199:1213	arg1	mixture					1215:1221	a novel oligosaccharide mixture	1191:1221	a novel oligosaccharide mixture	1191:1221	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	1	33	theme	polymerization	207:220	arg1	products					242:249	products	242:249	products of Lactobacillus reuteri glucansucrases	242:289	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	33	theme	polymerization	207:220	arg1	degree					197:202	a degree	195:202	a degree of polymerization (DP) of 3-4 (GL34)	195:239	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	0	34	theme	glucosylated	29:40	arg1	derivatives					50:60	novel glucosylated lactose derivatives	23:60	novel glucosylated lactose derivatives	23:60	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	1	35	theme	glucansucrases	276:289	arg1	products					242:249	products	242:249	products of Lactobacillus reuteri glucansucrases	242:289	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	35	theme	glucansucrases	276:289	arg1	degree					197:202	a degree	195:202	a degree of polymerization (DP) of 3-4 (GL34)	195:239	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	2	36	theme	carbohydrate-degrading	433:454	arg1	enzymes					456:462	common carbohydrate-degrading enzymes	426:462	common carbohydrate-degrading enzymes	426:462	Here, we show that these GL34 compounds are largely resistant to the hydrolytic activities of common carbohydrate-degrading enzymes.					
30406451	7	37	theme	GL34	1118:1121	arg1	compounds					1123:1131	the five GL34 compounds	1109:1131	the five GL34 compounds	1109:1131	Bifidobacterium adolescentis was most effective, completely degrading four of the five GL34 compounds, leaving only one minor constituent.					
30406451	2	38	theme	common	426:431	arg1	enzymes					456:462	common carbohydrate-degrading enzymes	426:462	common carbohydrate-degrading enzymes	426:462	Here, we show that these GL34 compounds are largely resistant to the hydrolytic activities of common carbohydrate-degrading enzymes.					
30406451	7	39	theme	minor	1151:1155	arg1	constituent					1157:1167	only one minor constituent	1142:1167	only one minor constituent	1142:1167	Bifidobacterium adolescentis was most effective, completely degrading four of the five GL34 compounds, leaving only one minor constituent.					
30406451	0	40	theme	derivatives	50:60	arg1	effects					12:18	Stimulatory effects	0:18	Stimulatory effects of novel glucosylated lactose derivatives	0:60	Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria.					
30406451	8	41	with	mixture	1215:1221	arg1	potential					1229:1237	potential	1229:1237	potential	1229:1237	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	8	41	with	mixture	1215:1221	arg1	properties					1250:1259	(potential) synbiotic properties	1228:1259	(potential) synbiotic properties towards B. adolescentis	1228:1283	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	3	42	theme	commensal	552:560	arg1	bacteria					562:569	commensal bacteria	552:569	commensal bacteria	552:569	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	8	43	theme	synbiotic	1240:1248	arg1	potential					1229:1237	potential	1229:1237	potential	1229:1237	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	8	43	theme	synbiotic	1240:1248	arg1	properties					1250:1259	(potential) synbiotic properties	1228:1259	(potential) synbiotic properties towards B. adolescentis	1228:1283	GL34 thus represents a novel oligosaccharide mixture with (potential) synbiotic properties towards B. adolescentis, synthesized from cheap and abundantly available lactose and sucrose.					
30406451	4	44	theme	commensal	692:700	arg1	bacteria					702:709	commensal bacteria	692:709	commensal bacteria	692:709	Bifidobacteria clearly grew better on the GL34 mixture than lactobacilli and commensal bacteria.					
30406451	5	45	theme	w/w	907:909	arg1	%					905:905	30% w/w	903:909	30% w/w of GL34	903:917	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	45	theme	w/w	907:909	arg1	GL34					914:917	GL34	914:917	GL34	914:917	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	46	theme	GL34	914:917	arg1	%					905:905	30% w/w	903:909	30% w/w of GL34	903:917	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	46	theme	GL34	914:917	arg1	GL34					914:917	GL34	914:917	GL34	914:917	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	1	47	theme	lactose	162:168	arg1	F1-F5					183:187	F1-F5	183:187	F1-F5	183:187	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	47	theme	lactose	162:168	arg1	substrates					320:329	substrates	320:329	substrates	320:329	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	47	theme	lactose	162:168	arg1	derivatives					170:180	five glucosylated lactose derivatives	144:180	five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose	144:315	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	6	48	theme	GL34	976:979	arg1	mixture					981:987	the GL34 mixture	972:987	the GL34 mixture	972:987	Bifidobacteria digested more than one compound from the GL34 mixture, varying with the specific strain tested.					
30406451	1	49	theme	glucosylated	149:160	arg1	F1-F5					183:187	F1-F5	183:187	F1-F5	183:187	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	49	theme	glucosylated	149:160	arg1	substrates					320:329	substrates	320:329	substrates	320:329	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	49	theme	glucosylated	149:160	arg1	derivatives					170:180	five glucosylated lactose derivatives	144:180	five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose	144:315	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	5	50	theme	commensal	733:741	arg1	bacteria					743:750	the commensal bacteria	729:750	the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron	729:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	50	theme	commensal	733:741	arg1	Nissle					769:774	Escherichia coli Nissle	752:774	Escherichia coli Nissle	752:774	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	5	50	theme	commensal	733:741	arg1	thetaiotaomicron					792:807	Bacteroides thetaiotaomicron	780:807	Bacteroides thetaiotaomicron	780:807	Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glcp-(1 → 2)-[β-D-Galp-(1 → 4)-]D-Glcp, constituting around 30% w/w of GL34.					
30406451	1	51	dep	Lactobacillus	254:266	arg1	reuteri					268:274	reuteri	268:274	reuteri	268:274	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	3	52	theme	strains	493:499	arg1	ability					475:481	the ability	471:481	the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds	471:600	Also, the ability of single strains of gut bacteria, bifidobacteria, lactobacilli, and commensal bacteria, to ferment the GL34 compounds was studied.					
30406451	1	53	theme	3-4	230:232	arg1	DP					223:224	DP	223:224	DP	223:224	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30406451	1	53	theme	3-4	230:232	arg1	polymerization					207:220	polymerization	207:220	polymerization (DP) of 3-4 (GL34)	207:239	Previously we structurally characterized five glucosylated lactose derivatives (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34), products of Lactobacillus reuteri glucansucrases, with lactose and sucrose as substrates.					
30179797	6	0	theme	acid	966:969	arg1	results					999:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	2	1	from	Lucidum	448:454	arg1	polysaccharides					417:431	polysaccharides	417:431	polysaccharides from Ganoderma Lucidum (GLP)	417:460	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	1	from	Lucidum	448:454	arg1	interactions					401:412	the molecular interactions	387:412	the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA)	387:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	10	2	with	interactions	1617:1628	arg1	protein					1654:1660	protein	1654:1660	protein	1654:1660	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	4	3	theme	conformational	730:743	arg1	changes					745:751	the conformational changes	726:751	the conformational changes of protein	726:762	The results showed that the formation of GLP-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein.					
30179797	7	4	with	formation	1166:1174	arg1	rate					1214:1217	a lower rate	1206:1217	a lower rate	1206:1217	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	4	5	theme	complex	672:678	arg1	formation					651:659	the formation	647:659	the formation of GLP-BSA complex by mainly hydrogen-bonding forces	647:712	The results showed that the formation of GLP-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein.					
30179797	0	6	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.	0:132	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	5	7	theme	conformational	872:885	arg1	state					887:891	a novel and more stable conformational state	848:891	a novel and more stable conformational state	848:891	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	2	8	theme	multi-spectral	321:334	arg1	methods					336:342	multi-spectral methods	321:342	multi-spectral methods	321:342	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	1	9	theme	numerous	206:213	arg1	applications					230:241	numerous very important applications	206:241	numerous very important applications for pharmaceutical, food, chemical and other industries	206:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	0	10	theme	serum	119:123	arg1	albumin					125:131	bovine serum albumin	112:131	bovine serum albumin	112:131	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	5	11	theme	novel	850:854	arg1	state					887:891	a novel and more stable conformational state	848:891	a novel and more stable conformational state	848:891	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	8	12	theme	fluorescence	1224:1235	arg1	spectra					1237:1243	The fluorescence spectra	1220:1243	The fluorescence spectra of BSA glycosylated by GLP	1220:1270	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	2	13	theme	molecular	391:399	arg1	interactions					401:412	the molecular interactions	387:412	the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA)	387:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	7	14	theme	beta	1179:1182	arg1	structures					1190:1199	beta sheet structures	1179:1199	beta sheet structures	1179:1199	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	6	15	theme	fluorescence	977:988	arg1	results					999:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	8	16	theme	bonds	1308:1312	arg1	formation					1286:1294	the formation	1282:1294	the formation of covalent bonds between BSA and GLP	1282:1332	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	8	17	dep	transform	1434:1442	arg1	infrared					1444:1451	infrared	1444:1451	transform infrared	1434:1451	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	6	18	theme	low	1068:1070	arg1	ability					1080:1086	low binding ability	1068:1086	low binding ability with ANS	1068:1095	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	0	19	theme	polysaccharides	63:77	arg1	effects					34:40	the effects	30:40	the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin	30:131	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	9	20	theme	good	1521:1524	arg1	activity					1526:1533	the esterase-like good activity	1503:1533	the esterase-like good activity	1503:1533	In addition, BSA still maintains the esterase-like good activity in the presence of GLP.					
30179797	6	21	theme	intermediate	1039:1050	arg1	state					1052:1056	some intermediate state	1034:1056	some intermediate state which has low binding ability with ANS in the presence of GLP	1034:1118	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	2	22	theme	polysaccharides	417:431	arg1	interactions					401:412	the molecular interactions	387:412	the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA)	387:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	7	23	theme	lower	1208:1212	arg1	rate					1214:1217	a lower rate	1206:1217	a lower rate	1206:1217	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	9	24	theme	GLP	1554:1556	arg1	presence					1542:1549	the presence	1538:1549	the presence of GLP	1538:1556	In addition, BSA still maintains the esterase-like good activity in the presence of GLP.					
30179797	2	25	theme	bovine	467:472	arg1	albumin					480:486	bovine serum albumin	467:486	bovine serum albumin (BSA)	467:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	25	theme	bovine	467:472	arg1	BSA					489:491	BSA	489:491	BSA	489:491	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	0	26	from	effects	34:40	arg1	activity					100:107	activity	100:107	activity	100:107	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	0	26	from	effects	34:40	arg1	structure					86:94	structure	86:94	structure	86:94	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	5	27	theme	BSA	828:830	arg1	solution					832:839	BSA solution	828:839	BSA solution having a novel and more stable conformational state during the thermal denaturation process	828:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	10	28	theme	important	1686:1694	arg1	ingredients					1708:1718	important food active ingredients	1686:1718	important food active ingredients	1686:1718	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	7	29	theme	sheet	1184:1188	arg1	structures					1190:1199	beta sheet structures	1179:1199	beta sheet structures	1179:1199	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	2	30	theme	Ganoderma	438:446	arg1	GLP					457:459	GLP	457:459	GLP	457:459	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	30	theme	Ganoderma	438:446	arg1	Lucidum					448:454	Ganoderma Lucidum	438:454	Ganoderma Lucidum (GLP)	438:460	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	0	31	theme	effects	34:40	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.	0:132	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	2	32	from	interactions	401:412	arg1	GLP					457:459	GLP	457:459	GLP	457:459	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	32	from	interactions	401:412	arg1	Lucidum					448:454	Ganoderma Lucidum	438:454	Ganoderma Lucidum (GLP)	438:460	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	0	33	theme	Ganoderma	45:53	arg1	Lucidum					55:61	Ganoderma Lucidum	45:61	Ganoderma Lucidum polysaccharides	45:77	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	7	34	from	decrease	1150:1157	arg1	formation					1166:1174	the formation	1162:1174	the formation of beta sheet structures with a lower rate	1162:1217	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	8	35	theme	Fourier	1426:1432	arg1	analysis					1460:1467	thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis	1398:1467	thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis	1398:1467	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	2	36	theme	molecular	348:356	arg1	docking					358:364	molecular docking	348:364	molecular docking	348:364	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	1	37	theme	pharmaceutical	247:260	arg1	industries					288:297	pharmaceutical, food, chemical and other industries	247:297	pharmaceutical, food, chemical and other industries	247:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	5	38	theme	thermal	904:910	arg1	denaturation					912:923	the thermal denaturation	900:923	the thermal denaturation process	900:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	4	39	theme	GLP-BSA	664:670	arg1	complex					672:678	GLP-BSA complex	664:678	GLP-BSA complex	664:678	The results showed that the formation of GLP-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein.					
30179797	1	40	theme	food	263:266	arg1	industries					288:297	pharmaceutical, food, chemical and other industries	247:297	pharmaceutical, food, chemical and other industries	247:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	0	41	theme	Lucidum	55:61	arg1	polysaccharides					63:77	Ganoderma Lucidum polysaccharides	45:77	Ganoderma Lucidum polysaccharides	45:77	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	2	42	with	interactions	401:412	arg1	albumin					480:486	bovine serum albumin	467:486	bovine serum albumin (BSA)	467:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	42	with	interactions	401:412	arg1	BSA					489:491	BSA	489:491	BSA	489:491	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	1	43	theme	chemical	269:276	arg1	industries					288:297	pharmaceutical, food, chemical and other industries	247:297	pharmaceutical, food, chemical and other industries	247:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	6	44	theme	8-anilino-1-naphthalenesulfonic	934:964	arg1	ANS					972:974	ANS	972:974	ANS	972:974	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	6	44	theme	8-anilino-1-naphthalenesulfonic	934:964	arg1	acid					966:969	8-anilino-1-naphthalenesulfonic acid	934:969	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	7	45	theme	GLP	1137:1139	arg1	presence					1125:1132	The presence	1121:1132	The presence of GLP	1121:1139	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	2	46	theme	present	307:313	arg1	work					315:318	the present work	303:318	the present work	303:318	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	1	47	theme	other	282:286	arg1	industries					288:297	pharmaceutical, food, chemical and other industries	247:297	pharmaceutical, food, chemical and other industries	247:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	5	48	theme	denaturation	912:923	arg1	process					925:931	the thermal denaturation process	900:931	the thermal denaturation process	900:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	3	49	theme	thermal	541:547	arg1	stability					549:557	thermal stability	541:557	thermal stability	541:557	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	5	50	theme	stable	865:870	arg1	state					887:891	a novel and more stable conformational state	848:891	a novel and more stable conformational state	848:891	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	8	51	theme	BSA	1248:1250	arg1	spectra					1237:1243	The fluorescence spectra	1220:1243	The fluorescence spectra of BSA glycosylated by GLP	1220:1270	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	0	52	theme	albumin	125:131	arg1	activity					100:107	activity	100:107	activity	100:107	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	0	52	theme	albumin	125:131	arg1	structure					86:94	structure	86:94	structure	86:94	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	3	53	theme	structure	564:572	arg1	information					574:584	structure information	564:584	structure information	564:584	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	3	54	theme	GLP-BSA	589:595	arg1	system					597:602	GLP-BSA system	589:602	GLP-BSA system	589:602	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	5	55	contain	having	841:846	arg2	state					887:891	a novel and more stable conformational state	848:891	a novel and more stable conformational state	848:891	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	5	55	contain	having	841:846	arg1	solution					832:839	BSA solution	828:839	BSA solution having a novel and more stable conformational state during the thermal denaturation process	828:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	10	56	theme	ingredients	1708:1718	arg1	perspective					1671:1681	the perspective	1667:1681	the perspective of important food active ingredients	1667:1718	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	8	57	gly	glycosylated	1252:1263	arg1	BSA					1248:1250	BSA	1248:1250	BSA glycosylated by GLP	1248:1270	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	6	58	theme	spectral	990:997	arg1	results					999:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results	934:1005	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	10	59	theme	molecular	1607:1615	arg1	interactions					1617:1628	the molecular interactions	1603:1628	the molecular interactions of polysaccharides with protein	1603:1660	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	3	60	theme	system	597:602	arg1	information					574:584	structure information	564:584	structure information	564:584	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	3	60	theme	system	597:602	arg1	fibrillation					527:538	fibrillation	527:538	fibrillation	527:538	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	3	60	theme	system	597:602	arg1	stability					549:557	thermal stability	541:557	thermal stability	541:557	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	3	60	theme	system	597:602	arg1	glucosylation					512:524	The nonenzymatic glucosylation	495:524	The nonenzymatic glucosylation	495:524	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	8	61	theme	covalent	1299:1306	arg1	bonds					1308:1312	covalent bonds	1299:1312	covalent bonds	1299:1312	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	8	62	dep	Fourier	1426:1432	arg1	transform					1434:1442	transform	1434:1442	transform infrared	1434:1451	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	6	63	theme	binding	1072:1078	arg1	ability					1080:1086	low binding ability	1068:1086	low binding ability with ANS	1068:1095	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	0	64	from	activity	100:107	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.	0:132	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	10	65	theme	active	1701:1706	arg1	ingredients					1708:1718	important food active ingredients	1686:1718	important food active ingredients	1686:1718	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	9	66	theme	esterase-like	1507:1519	arg1	activity					1526:1533	the esterase-like good activity	1503:1533	the esterase-like good activity	1503:1533	In addition, BSA still maintains the esterase-like good activity in the presence of GLP.					
30179797	4	67	theme	hydrogen-bonding	690:705	arg1	forces					707:712	mainly hydrogen-bonding forces	683:712	mainly hydrogen-bonding forces	683:712	The results showed that the formation of GLP-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein.					
30179797	1	68	theme	polysaccharides-protein	158:180	arg1	system					182:187	polysaccharides-protein system	158:187	polysaccharides-protein system	158:187	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	0	69	from	characterization	10:25	arg1	activity					100:107	activity	100:107	activity	100:107	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	0	69	from	characterization	10:25	arg1	structure					86:94	structure	86:94	structure	86:94	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	2	70	used	used	371:374	arg2	methods					336:342	multi-spectral methods	321:342	multi-spectral methods	321:342	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	70	used	used	371:374	arg2	docking					358:364	molecular docking	348:364	molecular docking	348:364	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	0	71	from	structure	86:94	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.	0:132	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	8	72	theme	Maillard	1346:1353	arg1	reaction					1355:1362	the Maillard reaction	1342:1362	the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis	1342:1467	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	6	73	contain	has	1064:1066	arg2	ability					1080:1086	low binding ability	1068:1086	low binding ability with ANS	1068:1095	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	6	73	contain	has	1064:1066	arg1	state					1052:1056	some intermediate state	1034:1056	some intermediate state which has low binding ability with ANS in the presence of GLP	1034:1118	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	0	74	theme	bovine	112:117	arg1	albumin					125:131	bovine serum albumin	112:131	bovine serum albumin	112:131	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	1	75	theme	important	220:228	arg1	applications					230:241	numerous very important applications	206:241	numerous very important applications for pharmaceutical, food, chemical and other industries	206:297	The investigation about polysaccharides-protein system is attributed to numerous very important applications for pharmaceutical, food, chemical and other industries.					
30179797	6	76	theme	GLP	1116:1118	arg1	presence					1104:1111	the presence	1100:1111	the presence of GLP	1100:1118	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	7	77	theme	structures	1190:1199	arg1	formation					1166:1174	the formation	1162:1174	the formation of beta sheet structures with a lower rate	1162:1217	The presence of GLP caused a decrease in the formation of beta sheet structures with a lower rate.					
30179797	0	78	dep	structure	86:94	arg1	the					82:84	the	82:84	the	82:84	Molecular characterization of the effects of Ganoderma Lucidum polysaccharides on the structure and activity of bovine serum albumin.					
30179797	4	79	theme	protein	756:762	arg1	changes					745:751	the conformational changes	726:751	the conformational changes of protein	726:762	The results showed that the formation of GLP-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein.					
30179797	10	80	theme	polysaccharides	1633:1647	arg1	interactions					1617:1628	the molecular interactions	1603:1628	the molecular interactions of polysaccharides with protein	1603:1660	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30179797	5	81	theme	solution	832:839	arg1	stability					815:823	the thermal stability	803:823	the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process	803:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	8	82	theme	thermogravimetric	1398:1414	arg1	analysis					1460:1467	thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis	1398:1467	thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis	1398:1467	The fluorescence spectra of BSA glycosylated by GLP confirmed the formation of covalent bonds between BSA and GLP through the Maillard reaction which was also confirmed by using thermogravimetric (TGA) and Fourier transform infrared (FTIR) analysis.					
30179797	5	83	theme	thermal	807:813	arg1	stability					815:823	the thermal stability	803:823	the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process	803:931	GLP acted as a stabilizer to increase the thermal stability of BSA solution having a novel and more stable conformational state during the thermal denaturation process.					
30179797	2	84	theme	serum	474:478	arg1	albumin					480:486	bovine serum albumin	467:486	bovine serum albumin (BSA)	467:492	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	2	84	theme	serum	474:478	arg1	BSA					489:491	BSA	489:491	BSA	489:491	In the present work, multi-spectral methods and molecular docking were used to analyze the molecular interactions of polysaccharides from Ganoderma Lucidum (GLP) with bovine serum albumin (BSA).					
30179797	3	85	theme	nonenzymatic	499:510	arg1	glucosylation					512:524	The nonenzymatic glucosylation	495:524	The nonenzymatic glucosylation	495:524	The nonenzymatic glucosylation, fibrillation, thermal stability, and structure information of GLP-BSA system were also studied.					
30179797	6	86	with	ability	1080:1086	arg1	ANS					1093:1095	ANS	1093:1095	ANS	1093:1095	8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectral results suggested that there exist some intermediate state which has low binding ability with ANS in the presence of GLP.					
30179797	10	87	theme	food	1696:1699	arg1	ingredients					1708:1718	important food active ingredients	1686:1718	important food active ingredients	1686:1718	These results provide a basis for screening the molecular interactions of polysaccharides with protein from the perspective of important food active ingredients.					
30375138	2	0	gly	glycosylation	486:498	arg1	solution					511:518	aqueous solution	503:518	aqueous solution	503:518	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	2	1	from	glycosylation	486:498	arg1	solution					511:518	aqueous solution	503:518	aqueous solution	503:518	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	2	2	theme	automated	408:416	arg1	system					418:423	a fully automated system	400:423	a fully automated system	400:423	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	1	3	theme	available	285:293	arg1	machines					295:302	commercially available machines	272:302	commercially available machines	272:302	For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines.					
30375138	3	4	theme	thermosensitive	566:580	arg1	polymer					582:588	a thermosensitive polymer	564:588	a thermosensitive polymer	564:588	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	3	5	theme	available	609:617	arg1	synthesizer					627:637	a commercially available peptide synthesizer	594:637	a commercially available peptide synthesizer	594:637	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	3	6	theme	peptide	619:625	arg1	synthesizer					627:637	a commercially available peptide synthesizer	594:637	a commercially available peptide synthesizer	594:637	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	1	7	theme	automated	145:153	arg1	system					155:160	a general automated system	135:160	a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines	135:302	For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines.					
30375138	4	8	theme	proof-of-concept	664:679	arg1	demonstration					681:693	a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer	662:832	a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer	662:832	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30375138	2	9	theme	oligosaccharides	441:456	arg1	synthesis					458:466	oligosaccharides synthesis	441:466	oligosaccharides synthesis	441:466	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	0	10	theme	Enzymatic	15:23	arg1	Synthesis					41:49	Enzymatic Oligosaccharide Synthesis	15:49	Enzymatic Oligosaccharide Synthesis	15:49	Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer.					
30375138	4	11	theme	automated	766:774	arg1	manner					776:781	an automated manner	763:781	an automated manner using a commercially available peptide synthesizer	763:832	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30375138	2	12	theme	synthesis	358:366	arg1	success					321:327	the success	317:327	the success of automated oligosaccharide synthesis through chemical glycosylation	317:397	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	2	13	theme	oligosaccharide	342:356	arg1	synthesis					358:366	automated oligosaccharide synthesis	332:366	automated oligosaccharide synthesis	332:366	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	4	14	theme	oligosaccharides	727:742	arg1	synthesis					714:722	the enzymatic synthesis	700:722	the enzymatic synthesis of oligosaccharides	700:742	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30375138	2	15	theme	aqueous	503:509	arg1	solution					511:518	aqueous solution	503:518	aqueous solution	503:518	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	2	16	theme	automated	332:340	arg1	synthesis					358:366	automated oligosaccharide synthesis	332:366	automated oligosaccharide synthesis	332:366	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	3	17	theme	designed	525:532	arg1	system					534:539	The designed system	521:539	The designed system	521:539	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	2	18	theme	enzymatic	476:484	arg1	glycosylation					486:498	enzymatic glycosylation	476:498	enzymatic glycosylation in aqueous solution	476:518	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	1	19	theme	oligonucleotides	234:249	arg1	preparation					219:229	the preparation	215:229	the preparation of oligonucleotides and oligopeptides by commercially available machines	215:302	For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines.					
30375138	4	20	theme	peptide	814:820	arg1	synthesizer					822:832	a commercially available peptide synthesizer	789:832	a commercially available peptide synthesizer	789:832	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30375138	0	21	theme	a Peptide	60:68	arg1	Synthesizer					70:80	a Peptide Synthesizer	60:80	a Peptide Synthesizer	60:80	Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer.					
30375138	4	22	theme	available	804:812	arg1	synthesizer					822:832	a commercially available peptide synthesizer	789:832	a commercially available peptide synthesizer	789:832	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30375138	1	23	theme	general	137:143	arg1	system					155:160	a general automated system	135:160	a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines	135:302	For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines.					
30375138	2	24	theme	chemical	376:383	arg1	glycosylation					385:397	chemical glycosylation	376:397	chemical glycosylation	376:397	Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution.					
30375138	1	25	theme	oligopeptides	255:267	arg1	preparation					219:229	the preparation	215:229	the preparation of oligonucleotides and oligopeptides by commercially available machines	215:302	For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines.					
30375138	3	26	theme	polymer	582:588	arg1	use					557:559	the use	553:559	the use of a thermosensitive polymer	553:588	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	3	26	theme	polymer	582:588	arg1	synthesizer					627:637	a commercially available peptide synthesizer	594:637	a commercially available peptide synthesizer	594:637	The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer.					
30375138	0	27	theme	Oligosaccharide	25:39	arg1	Synthesis					41:49	Enzymatic Oligosaccharide Synthesis	15:49	Enzymatic Oligosaccharide Synthesis	15:49	Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer.					
30375138	4	28	theme	enzymatic	704:712	arg1	synthesis					714:722	the enzymatic synthesis	700:722	the enzymatic synthesis of oligosaccharides	700:742	This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.					
30315106	9	0	theme	core	1585:1588	arg1	structures					1599:1608	core 1/core 2 structures	1585:1608	core 1/core 2 structures	1585:1608	We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms.					
30315106	7	1	theme	GalNAc	1187:1192	arg1	residues					1194:1201	GalNAc residues	1187:1201	GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1187:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	2	theme	repeated	1149:1156	arg1	sequences					1166:1174	repeated protein sequences	1149:1174	structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1060:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	3	theme	GalNAc-α1,3	1091:1101	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	3	theme	GalNAc-α1,3	1091:1101	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	3	4	theme	pancreatic	467:476	arg1	PDAC					501:504	PDAC	501:504	PDAC	501:504	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	4	theme	pancreatic	467:476	arg1	adenocarcinoma					485:498	pancreatic ductal adenocarcinoma	467:498	pancreatic ductal adenocarcinoma (PDAC)	467:505	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	1	5	theme	human	230:234	arg1	disease					236:242	human disease	230:242	human disease	230:242	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	10	6	theme	biological	1852:1861	arg1	functions					1863:1871	additional biological functions	1841:1871	additional biological functions	1841:1871	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	2	7	theme	Rare	245:248	arg1	mutations					250:258	Rare mutations	245:258	Rare mutations in the CEL gene	245:274	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	1	8	theme	Carboxyl-ester	149:162	arg1	lipase					164:169	Carboxyl-ester lipase	149:169	Carboxyl-ester lipase (CEL)	149:175	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	1	8	theme	Carboxyl-ester	149:162	arg1	enzyme					207:212	a pancreatic fat-digesting enzyme	180:212	a pancreatic fat-digesting enzyme associated with human disease	180:242	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	1	8	theme	Carboxyl-ester	149:162	arg1	CEL					172:174	CEL	172:174	CEL	172:174	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	0	9	theme	ABO	119:121	arg1	determinants					135:146	ABO blood group determinants	119:146	ABO blood group determinants	119:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	3	10	theme	CEL	538:540	arg1	variant					542:548	a postulated oncofetal CEL variant	515:548	a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP)	515:593	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	10	11	theme	cholesteryl	1880:1890	arg1	activity					1901:1908	its cholesteryl esterase activity	1876:1908	its cholesteryl esterase activity in the duodenum	1876:1924	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	3	12	theme	postulated	517:526	arg1	variant					542:548	a postulated oncofetal CEL variant	515:548	a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP)	515:593	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	5	13	theme	pancreatic	798:807	arg1	lesions					809:815	malignant pancreatic lesions	788:815	malignant pancreatic lesions	788:815	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	8	14	theme	group	1378:1382	arg1	antigens					1384:1391	blood group antigens	1372:1391	blood group antigens	1372:1391	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	0	15	mod	modified	107:114	arg3	determinants					135:146	ABO blood group determinants	119:146	ABO blood group determinants	119:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	15	mod	modified	107:114	arg1	O-glycans					85:93	core 1/core 2 O-glycans	71:93	core 1/core 2 O-glycans that can be modified by ABO blood group determinants	71:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	7	16	theme	glycan	1004:1009	arg1	microarrays					1011:1021	glycan microarrays	1004:1021	glycan microarrays	1004:1021	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	3	17	theme	pancreatic	569:578	arg1	FAPP					589:592	FAPP	589:592	FAPP	589:592	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	17	theme	pancreatic	569:578	arg1	protein					580:586	feto-acinar pancreatic protein	557:586	feto-acinar pancreatic protein (FAPP)	557:593	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	8	18	used	used	1397:1400	arg2	we					1394:1395	we	1394:1395	we	1394:1395	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	7	19	theme	antibody	992:999	arg1	Testing					968:974	Testing	968:974	Testing of the mAb16D10 antibody in glycan microarrays	968:1021	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	2	20	from	mutations	250:258	arg1	gene					271:274	the CEL gene	263:274	the CEL gene	263:274	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	9	21	theme	ABO	1665:1667	arg1	polymorphisms					1674:1686	ABO gene polymorphisms	1665:1686	ABO gene polymorphisms	1665:1686	We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms.					
30315106	7	22	theme	pancreatic	1292:1301	arg1	tissue					1303:1308	human pancreatic tissue	1286:1308	human pancreatic tissue	1286:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	4	23	theme	O-glycosylated	689:702	arg1	terminus					718:725	the highly O-glycosylated, mucin-like C terminus	678:725	the highly O-glycosylated, mucin-like C terminus of CEL/FAPP	678:737	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	0	24	theme	group	129:133	arg1	determinants					135:146	ABO blood group determinants	119:146	ABO blood group determinants	119:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	8	25	theme	MALDI-TOF	1419:1427	arg1	MS					1429:1430	high-sensitivity MALDI-TOF MS	1402:1430	high-sensitivity MALDI-TOF MS	1402:1430	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	6	26	theme	pancreatic	949:958	arg1	cancer					960:965	pancreatic cancer	949:965	pancreatic cancer	949:965	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	7	27	theme	protein	1158:1164	arg1	sequences					1166:1174	repeated protein sequences	1149:1174	structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1060:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	6	28	theme	CEL	942:944	arg1	FAPP					901:904	FAPP	901:904	FAPP	901:904	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	6	28	theme	CEL	942:944	arg1	glycoisoform					926:937	a glycoisoform	924:937	a glycoisoform of CEL in pancreatic cancer	924:965	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	4	29	theme	CEL/FAPP	730:737	arg1	terminus					718:725	the highly O-glycosylated, mucin-like C terminus	678:725	the highly O-glycosylated, mucin-like C terminus of CEL/FAPP	678:737	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	0	30	theme	1/core	76:81	arg1	O-glycans					85:93	core 1/core 2 O-glycans	71:93	core 1/core 2 O-glycans that can be modified by ABO blood group determinants	71:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	8	31	theme	O-glycan	1461:1468	arg1	pool					1470:1473	the released O-glycan pool	1448:1473	the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice	1448:1527	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	2	32	theme	chronic	412:418	arg1	pancreatitis					420:431	chronic pancreatitis	412:431	chronic pancreatitis	412:431	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	5	33	theme	human	775:779	arg1	CEL					781:783	human CEL	775:783	human CEL	775:783	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	10	34	theme	unique	1777:1782	arg1	characteristics					1784:1798	some unique characteristics	1772:1798	some unique characteristics	1772:1798	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	7	35	theme	blood	1117:1121	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	35	theme	blood	1117:1121	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	8	36	theme	human	1506:1510	arg1	juice					1523:1527	human pancreatic juice	1506:1527	human pancreatic juice	1506:1527	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	7	37	theme	A	1129:1129	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	37	theme	A	1129:1129	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	8	38	theme	CEL	1334:1336	arg1	glycoprotein					1338:1349	the CEL glycoprotein	1330:1349	the CEL glycoprotein	1330:1349	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	2	39	theme	CEL	378:380	arg1	allele					382:387	a recombined CEL allele	365:387	a recombined CEL allele	365:387	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	0	40	theme	pancreatic	23:32	arg1	CEL					57:59	CEL	57:59	CEL	57:59	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	40	theme	pancreatic	23:32	arg1	lipase					49:54	pancreatic carboxyl-ester lipase	23:54	pancreatic carboxyl-ester lipase (CEL)	23:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	2	41	theme	exocrine	307:314	arg1	dysfunction					330:340	pancreatic exocrine and endocrine dysfunction	296:340	pancreatic exocrine and endocrine dysfunction denoted MODY8	296:354	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	7	42	theme	Fuc-α1,2	1103:1110	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	42	theme	Fuc-α1,2	1103:1110	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	1	43	theme	pancreatic	182:191	arg1	enzyme					207:212	a pancreatic fat-digesting enzyme	180:212	a pancreatic fat-digesting enzyme associated with human disease	180:242	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	1	43	theme	pancreatic	182:191	arg1	lipase					164:169	Carboxyl-ester lipase	149:169	Carboxyl-ester lipase (CEL)	149:175	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	3	44	attach	linked	457:462	arg2	CEL					444:446	CEL	444:446	CEL	444:446	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	44	attach	linked	457:462	arg1	PDAC					501:504	PDAC	501:504	PDAC	501:504	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	44	attach	linked	457:462	arg1	adenocarcinoma					485:498	pancreatic ductal adenocarcinoma	467:498	pancreatic ductal adenocarcinoma (PDAC)	467:505	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	8	45	theme	blood	1372:1376	arg1	antigens					1384:1391	blood group antigens	1372:1391	blood group antigens	1372:1391	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	3	46	theme	ductal	478:483	arg1	PDAC					501:504	PDAC	501:504	PDAC	501:504	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	46	theme	ductal	478:483	arg1	adenocarcinoma					485:498	pancreatic ductal adenocarcinoma	467:498	pancreatic ductal adenocarcinoma (PDAC)	467:505	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	8	47	gly	glycoprotein	1338:1349	arg1	glycoprotein					1338:1349	the CEL glycoprotein	1330:1349	the CEL glycoprotein	1330:1349	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	0	48	theme	mucinous	4:11	arg1	domain					13:18	The mucinous domain	0:18	The mucinous domain of pancreatic carboxyl-ester lipase (CEL)	0:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	48	theme	mucinous	4:11	arg1	lipase					49:54	pancreatic carboxyl-ester lipase	23:54	pancreatic carboxyl-ester lipase (CEL)	23:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	7	49	attach	linked	1203:1208	arg1	findings					1235:1242	findings	1235:1242	findings that were supported by immunostainings of human pancreatic tissue	1235:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	49	attach	linked	1203:1208	arg1	Ser/Thr					1213:1219	Ser/Thr	1213:1219	Ser/Thr (Tn antigen)	1213:1232	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	49	attach	linked	1203:1208	arg2	residues					1194:1201	GalNAc residues	1187:1201	GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1187:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	49	attach	linked	1203:1208	arg1	antigen					1225:1231	Tn antigen	1222:1231	Tn antigen	1222:1231	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	50	theme	terminal	1082:1089	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	50	theme	terminal	1082:1089	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	2	51	theme	endocrine	320:328	arg1	dysfunction					330:340	pancreatic exocrine and endocrine dysfunction	296:340	pancreatic exocrine and endocrine dysfunction denoted MODY8	296:354	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	6	52	theme	neoplastic	869:878	arg1	cells					880:884	neoplastic cells	869:884	neoplastic cells	869:884	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	9	53	theme	1/core	1590:1595	arg1	structures					1599:1608	core 1/core 2 structures	1585:1608	core 1/core 2 structures	1585:1608	We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms.					
30315106	5	54	theme	cell	821:824	arg1	lines					826:830	cell lines	821:830	cell lines	821:830	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	0	55	theme	lipase	49:54	arg1	domain					13:18	The mucinous domain	0:18	The mucinous domain of pancreatic carboxyl-ester lipase (CEL)	0:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	55	theme	lipase	49:54	arg1	lipase					49:54	pancreatic carboxyl-ester lipase	23:54	pancreatic carboxyl-ester lipase (CEL)	23:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	8	56	mod	modified	1360:1367	arg1	glycoprotein					1338:1349	the CEL glycoprotein	1330:1349	the CEL glycoprotein	1330:1349	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	8	56	mod	modified	1360:1367	arg3	antigens					1384:1391	blood group antigens	1372:1391	blood group antigens	1372:1391	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	10	57	theme	additional	1841:1850	arg1	functions					1863:1871	additional biological functions	1841:1871	additional biological functions	1841:1871	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	3	58	theme	oncofetal	528:536	arg1	variant					542:548	a postulated oncofetal CEL variant	515:548	a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP)	515:593	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	10	59	theme	esterase	1892:1899	arg1	activity					1901:1908	its cholesteryl esterase activity	1876:1908	its cholesteryl esterase activity in the duodenum	1876:1924	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	10	60	theme	digestive	1701:1709	arg1	enzymes					1711:1717	digestive enzymes	1701:1717	digestive enzymes secreted by the pancreas	1701:1742	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	5	61	theme	malignant	788:796	arg1	lesions					809:815	malignant pancreatic lesions	788:815	malignant pancreatic lesions	788:815	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	3	62	theme	feto-acinar	557:567	arg1	FAPP					589:592	FAPP	589:592	FAPP	589:592	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	3	62	theme	feto-acinar	557:567	arg1	protein					580:586	feto-acinar pancreatic protein	557:586	feto-acinar pancreatic protein (FAPP)	557:593	Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP).					
30315106	4	63	theme	monoclonal	600:609	arg1	antibody					611:618	The monoclonal antibody mAb16D10	596:627	The monoclonal antibody mAb16D10	596:627	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	0	64	theme	blood	123:127	arg1	determinants					135:146	ABO blood group determinants	119:146	ABO blood group determinants	119:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	10	65	with	glycoprotein	1754:1765	arg1	characteristics					1784:1798	some unique characteristics	1772:1798	some unique characteristics	1772:1798	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	8	66	theme	high-sensitivity	1402:1417	arg1	MS					1429:1430	high-sensitivity MALDI-TOF MS	1402:1430	high-sensitivity MALDI-TOF MS	1402:1430	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	9	67	theme	gene	1669:1672	arg1	polymorphisms					1674:1686	ABO gene polymorphisms	1665:1686	ABO gene polymorphisms	1665:1686	We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms.					
30315106	7	68	theme	tissue	1303:1308	arg1	immunostainings					1267:1281	immunostainings	1267:1281	immunostainings of human pancreatic tissue	1267:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	69	theme	human	1286:1290	arg1	tissue					1303:1308	human pancreatic tissue	1286:1308	human pancreatic tissue	1286:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	70	contain	containing	1071:1080	arg2	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	70	contain	containing	1071:1080	arg2	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	70	contain	containing	1071:1080	arg1	structures					1060:1069	structures	1060:1069	structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1060:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	0	71	theme	core	71:74	arg1	O-glycans					85:93	core 1/core 2 O-glycans	71:93	core 1/core 2 O-glycans that can be modified by ABO blood group determinants	71:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	5	72	from	expression	761:770	arg1	lesions					809:815	malignant pancreatic lesions	788:815	malignant pancreatic lesions	788:815	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	5	72	from	expression	761:770	arg1	lines					826:830	cell lines	821:830	cell lines	821:830	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	8	73	theme	released	1452:1459	arg1	pool					1470:1473	the released O-glycan pool	1448:1473	the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice	1448:1527	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	10	74	from	activity	1901:1908	arg1	duodenum					1917:1924	the duodenum	1913:1924	the duodenum	1913:1924	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	2	75	theme	CEL	267:269	arg1	gene					271:274	the CEL gene	263:274	the CEL gene	263:274	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	8	76	theme	CEL	1478:1480	arg1	pool					1470:1473	the released O-glycan pool	1448:1473	the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice	1448:1527	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	6	77	from	glycoisoform	926:937	arg1	cancer					960:965	pancreatic cancer	949:965	pancreatic cancer	949:965	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	10	78	gly	glycoprotein	1754:1765	arg1	CEL					1745:1747	CEL	1745:1747	CEL	1745:1747	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	10	78	gly	glycoprotein	1754:1765	arg1	glycoprotein					1754:1765	a glycoprotein	1752:1765	a glycoprotein with some unique characteristics	1752:1798	Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.					
30315106	7	79	theme	group	1123:1127	arg1	antigen					1131:1137	blood group A antigen	1117:1137	blood group A antigen	1117:1137	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	79	theme	group	1123:1127	arg1	Gal					1112:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal	1082:1114	terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen)	1082:1138	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	4	80	dep	O-glycosylated	689:702	arg1	mucin-like					705:714	mucin-like	705:714	mucin-like	705:714	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	5	81	theme	CEL	781:783	arg1	expression					761:770	the expression	757:770	the expression of human CEL in malignant pancreatic lesions and cell lines	757:830	We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines.					
30315106	8	82	theme	pancreatic	1512:1521	arg1	juice					1523:1527	human pancreatic juice	1506:1527	human pancreatic juice	1506:1527	To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice.					
30315106	4	83	theme	C	716:716	arg1	terminus					718:725	the highly O-glycosylated, mucin-like C terminus	678:725	the highly O-glycosylated, mucin-like C terminus of CEL/FAPP	678:737	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	0	84	theme	carboxyl-ester	34:47	arg1	CEL					57:59	CEL	57:59	CEL	57:59	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	84	theme	carboxyl-ester	34:47	arg1	lipase					49:54	pancreatic carboxyl-ester lipase	23:54	pancreatic carboxyl-ester lipase (CEL)	23:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	2	85	theme	recombined	367:376	arg1	allele					382:387	a recombined CEL allele	365:387	a recombined CEL allele	365:387	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	7	86	theme	Tn	1222:1223	arg1	Ser/Thr					1213:1219	Ser/Thr	1213:1219	Ser/Thr (Tn antigen)	1213:1232	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	86	theme	Tn	1222:1223	arg1	antigen					1225:1231	Tn antigen	1222:1231	Tn antigen	1222:1231	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	4	87	gly	O-glycosylated	689:702	arg1	terminus					718:725	the highly O-glycosylated, mucin-like C terminus	678:725	the highly O-glycosylated, mucin-like C terminus of CEL/FAPP	678:737	The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP.					
30315106	6	88	gly	glycoisoform	926:937	arg1	CEL					942:944	CEL	942:944	CEL	942:944	CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer.					
30315106	2	89	theme	pancreatic	296:305	arg1	dysfunction					330:340	pancreatic exocrine and endocrine dysfunction	296:340	pancreatic exocrine and endocrine dysfunction denoted MODY8	296:354	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
30315106	9	90	theme	CEL	1561:1563	arg1	O-glycome					1548:1556	the O-glycome	1544:1556	the O-glycome of CEL	1544:1563	We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms.					
30315106	0	91	contain	contains	62:69	arg1	domain					13:18	The mucinous domain	0:18	The mucinous domain of pancreatic carboxyl-ester lipase (CEL)	0:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	91	contain	contains	62:69	arg2	O-glycans					85:93	core 1/core 2 O-glycans	71:93	core 1/core 2 O-glycans that can be modified by ABO blood group determinants	71:146	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	0	91	contain	contains	62:69	arg1	lipase					49:54	pancreatic carboxyl-ester lipase	23:54	pancreatic carboxyl-ester lipase (CEL)	23:60	The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants.					
30315106	1	92	theme	fat-digesting	193:205	arg1	enzyme					207:212	a pancreatic fat-digesting enzyme	180:212	a pancreatic fat-digesting enzyme associated with human disease	180:242	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	1	92	theme	fat-digesting	193:205	arg1	lipase					164:169	Carboxyl-ester lipase	149:169	Carboxyl-ester lipase (CEL)	149:175	Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease.					
30315106	7	93	from	Testing	968:974	arg1	microarrays					1011:1021	glycan microarrays	1004:1021	glycan microarrays	1004:1021	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	94	contain	containing	1176:1185	arg1	sequences					1166:1174	repeated protein sequences	1149:1174	structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1060:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	7	94	contain	containing	1176:1185	arg2	residues					1194:1201	GalNAc residues	1187:1201	GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue	1187:1308	Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-α1,3(Fuc-α1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue.					
30315106	2	95	theme	dysfunction	330:340	arg1	syndrome					284:291	a syndrome	282:291	a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8	282:354	Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis.					
29709182	0	0	theme	Streptococcus	60:72	arg1	Polysaccharide					83:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	1	theme	[4+2+1	385:390	arg1	glycosylation					393:405	convergent [4+2+1] glycosylation	374:405	convergent [4+2+1] glycosylation	374:405	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	2	theme	unit	145:148	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	2	theme	unit	145:148	arg1	p-					301:302	{[β-d-Gal p-	291:302	{[β-d-Gal p-(1→6)	291:307	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	2	theme	unit	145:148	arg1	p					318:318	-β-d-Gal p}	309:319	-β-d-Gal p}	309:319	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	2	theme	unit	145:148	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	2	theme	unit	145:148	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	3	theme	difficulties	480:491	arg1	series					470:475	a series	468:475	a series of difficulties	468:491	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	4	theme	p5Ac-	245:249	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	4	theme	p5Ac-	245:249	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	5	theme	B	58:58	arg1	Polysaccharide					83:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	4	6	theme	protected	963:971	arg1	donors					1029:1034	consecutive glycosyl donors	1008:1034	consecutive glycosyl donors	1008:1034	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	6	theme	protected	963:971	arg1	α-Neu5Ac-					982:990	α-Neu5Ac-	982:990	α-Neu5Ac-(2→3)	982:995	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	6	theme	protected	963:971	arg1	GlcN					973:976	properly protected GlcN	954:976	properly protected GlcN	954:976	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	0	7	theme	Polysaccharide	83:96	arg1	Unit					36:39	the Repeating Unit	22:39	the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide	22:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	8	theme	{	291:291	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	8	theme	{	291:291	arg1	p-					301:302	{[β-d-Gal p-	291:302	{[β-d-Gal p-(1→6)	291:307	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	8	theme	{	291:291	arg1	1→6					304:306	1→6	304:306	1→6	304:306	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	9	theme	hindered	860:867	arg1	acceptors					869:877	sterically hindered acceptors	849:877	sterically hindered acceptors	849:877	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	1	10	theme	-β-d-Glc	326:333	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	10	theme	-β-d-Glc	326:333	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	2	11	theme	downstream	561:570	arg1	end					572:574	its downstream end to enable further regioselective elaboration	557:619	its downstream end to enable further regioselective elaboration	557:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	1	12	theme	2→3	251:253	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	12	theme	2→3	251:253	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	13	theme	Capsular	74:81	arg1	Polysaccharide					83:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	14	theme	[β-d-Gal	292:299	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	14	theme	[β-d-Gal	292:299	arg1	p-					301:302	{[β-d-Gal p-	291:302	{[β-d-Gal p-(1→6)	291:307	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	14	theme	[β-d-Gal	292:299	arg1	1→6					304:306	1→6	304:306	1→6	304:306	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	15	theme	p-	335:336	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	15	theme	p-	335:336	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	16	theme	natural	721:727	arg1	glycans					729:735	natural glycans	721:735	natural glycans	721:735	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	2	17	theme	title	498:502	arg1	compound					504:511	The title compound	494:511	The title compound	494:511	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	1	18	theme	serotype	153:160	arg1	polysaccharide					196:209	serotype II group B Streptococcus capsular polysaccharide	153:209	serotype II group B Streptococcus capsular polysaccharide	153:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	4	19	theme	on-site	922:928	arg1	elongation					937:946	on-site glycan elongation	922:946	on-site glycan elongation	922:946	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	1	20	theme	1→3	338:340	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	20	theme	1→3	338:340	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	21	from	common	711:716	arg1	glycans					729:735	natural glycans	721:735	natural glycans	721:735	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	2	22	theme	regioselective	594:607	arg1	elaboration					609:619	further regioselective elaboration	586:619	further regioselective elaboration	586:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	4	23	theme	glycosyl	1020:1027	arg1	donors					1029:1034	consecutive glycosyl donors	1008:1034	consecutive glycosyl donors	1008:1034	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	23	theme	glycosyl	1020:1027	arg1	α-Neu5Ac-					982:990	α-Neu5Ac-	982:990	α-Neu5Ac-(2→3)	982:995	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	23	theme	glycosyl	1020:1027	arg1	GlcN					973:976	properly protected GlcN	954:976	properly protected GlcN	954:976	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	2	24	theme	further	586:592	arg1	elaboration					609:619	further regioselective elaboration	586:619	further regioselective elaboration	586:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	1	25	theme	group	165:169	arg1	polysaccharide					196:209	serotype II group B Streptococcus capsular polysaccharide	153:209	serotype II group B Streptococcus capsular polysaccharide	153:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	26	theme	Chemical	0:7	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.	0:97	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	27	theme	-β-d-Gal	255:262	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	27	theme	-β-d-Gal	255:262	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	28	theme	-β-d-Glc	342:349	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	28	theme	-β-d-Glc	342:349	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	29	theme	low	744:746	arg1	reactivity					748:757	a low reactivity	742:757	a low reactivity as glycosyl donors	742:776	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	1	30	theme	B	171:171	arg1	polysaccharide					196:209	serotype II group B Streptococcus capsular polysaccharide	153:209	serotype II group B Streptococcus capsular polysaccharide	153:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	31	theme	p-	264:265	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	31	theme	p-	264:265	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	32	theme	p-	351:352	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	32	theme	p-	351:352	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	33	theme	different	422:430	arg1	strategies					442:451	different synthetic strategies	422:451	different synthetic strategies	422:451	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	34	theme	Streptococcus	173:185	arg1	polysaccharide					196:209	serotype II group B Streptococcus capsular polysaccharide	153:209	serotype II group B Streptococcus capsular polysaccharide	153:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	35	contain	had	738:740	arg2	reactivity					748:757	a low reactivity	742:757	a low reactivity as glycosyl donors	742:776	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	3	35	contain	had	738:740	arg1	motif					695:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	3	35	contain	had	738:740	arg1	common					711:716	common	711:716	common	711:716	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	1	36	theme	synthetic	432:440	arg1	strategies					442:451	different synthetic strategies	422:451	different synthetic strategies	422:451	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	37	theme	capsular	187:194	arg1	polysaccharide					196:209	serotype II group B Streptococcus capsular polysaccharide	153:209	serotype II group B Streptococcus capsular polysaccharide	153:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	38	theme	Unit	36:39	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.	0:97	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	39	theme	1→4	267:269	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	39	theme	1→4	267:269	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	4	40	theme	glycan	930:935	arg1	elongation					937:946	on-site glycan elongation	922:946	on-site glycan elongation	922:946	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	1	41	theme	polysaccharide	196:209	arg1	unit					145:148	the repeating unit	131:148	the repeating unit of serotype II group B Streptococcus capsular polysaccharide	131:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	42	theme	Repeating	26:34	arg1	Unit					36:39	the Repeating Unit	22:39	the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide	22:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	2	43	contain	carry	529:533	arg1	compound					504:511	The title compound	494:511	The title compound	494:511	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	2	43	contain	carry	529:533	arg2	group					548:552	a free amino group	535:552	a free amino group at its downstream end to enable further regioselective elaboration	535:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	1	44	theme	-β-d-Gal	309:316	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	44	theme	-β-d-Gal	309:316	arg1	p					318:318	-β-d-Gal p}	309:319	-β-d-Gal p}	309:319	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	45	theme	Type	44:47	arg1	B					58:58	Type II Group B	44:58	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	46	theme	-β-d-GlcN	271:279	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	46	theme	-β-d-GlcN	271:279	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	47	theme	first	103:107	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	47	theme	first	103:107	arg1	p-					301:302	{[β-d-Gal p-	291:302	{[β-d-Gal p-(1→6)	291:307	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	47	theme	first	103:107	arg1	p					318:318	-β-d-Gal p}	309:319	-β-d-Gal p}	309:319	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	47	theme	first	103:107	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	47	theme	first	103:107	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	4	48	theme	consecutive	1008:1018	arg1	donors					1029:1034	consecutive glycosyl donors	1008:1034	consecutive glycosyl donors	1008:1034	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	48	theme	consecutive	1008:1018	arg1	α-Neu5Ac-					982:990	α-Neu5Ac-	982:990	α-Neu5Ac-(2→3)	982:995	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	4	48	theme	consecutive	1008:1018	arg1	GlcN					973:976	properly protected GlcN	954:976	properly protected GlcN	954:976	The motif was efficiently constructed via on-site glycan elongation using properly protected GlcN and α-Neu5Ac-(2→3)-β-d-Gal as consecutive glycosyl donors.					
29709182	1	49	theme	chemical	109:116	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	49	theme	chemical	109:116	arg1	p-					301:302	{[β-d-Gal p-	291:302	{[β-d-Gal p-(1→6)	291:307	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	49	theme	chemical	109:116	arg1	p					318:318	-β-d-Gal p}	309:319	-β-d-Gal p}	309:319	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	49	theme	chemical	109:116	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	49	theme	chemical	109:116	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	50	theme	-β-d-GlcNAc	683:693	arg1	motif					695:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	3	50	theme	-β-d-GlcNAc	683:693	arg1	common					711:716	common	711:716	common	711:716	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	2	51	theme	free	537:540	arg1	group					548:552	a free amino group	535:552	a free amino group at its downstream end to enable further regioselective elaboration	535:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	2	52	theme	amino	542:546	arg1	group					548:552	a free amino group	535:552	a free amino group at its downstream end to enable further regioselective elaboration	535:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	3	53	theme	glycosyl	762:769	arg1	donors					771:776	glycosyl donors	762:776	glycosyl donors	762:776	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	1	54	theme	branched	214:221	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	54	theme	branched	214:221	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	55	theme	Group	52:56	arg1	B					58:58	Type II Group B	44:58	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	56	theme	1→4	322:324	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	56	theme	1→4	322:324	arg1	1→					354:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	321:355	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	3	57	from	glycans	729:735	arg1	motif					695:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif	651:699	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	3	57	from	glycans	729:735	arg1	common					711:716	common	711:716	common	711:716	This work also revealed that the α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-GlcNAc motif, which is common in natural glycans, had a low reactivity as glycosyl donors, so it was rather difficult to directly couple this trisaccharide with sterically hindered acceptors.					
29709182	2	58	from	end	572:574	arg1	group					548:552	a free amino group	535:552	a free amino group at its downstream end to enable further regioselective elaboration	535:619	The title compound was designed to carry a free amino group at its downstream end to enable further regioselective elaboration.					
29709182	1	59	theme	heptasaccharide	223:237	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	59	theme	heptasaccharide	223:237	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	0	60	theme	II	49:50	arg1	B					58:58	Type II Group B	44:58	Type II Group B Streptococcus Capsular Polysaccharide	44:96	Chemical Synthesis of the Repeating Unit of Type II Group B Streptococcus Capsular Polysaccharide.					
29709182	1	61	theme	convergent	374:383	arg1	glycosylation					393:405	convergent [4+2+1] glycosylation	374:405	convergent [4+2+1] glycosylation	374:405	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	62	theme	repeating	135:143	arg1	unit					145:148	the repeating unit	131:148	the repeating unit of serotype II group B Streptococcus capsular polysaccharide	131:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	63	theme	α-Neu	239:243	arg1	synthesis					118:126	The first chemical synthesis	99:126	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide	99:209	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
29709182	1	63	theme	α-Neu	239:243	arg1	pAc-					281:284	a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→	212:355	pAc-	281:284	The first chemical synthesis of the repeating unit of serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming a series of difficulties.					
30380198	7	0	theme	replacement	1254:1264	arg1	effect					1225:1230	an effect	1222:1230	an effect of binding site water replacement on the binding affinity to l-ABP	1222:1297	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	3	1	theme	binding	489:495	arg1	site					497:500	the binding site	485:500	the binding site	485:500	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	5	2	theme	Glu	883:885	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	4	3	theme	FEP+	667:670	arg1	methodology					673:683	the free energy perturbation (FEP+) methodology	637:683	the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3	637:772	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	0	4	theme	Protein-Carbohydrate	83:102	arg1	Recognition					104:114	Noncovalent Protein-Carbohydrate Recognition	71:114	Noncovalent Protein-Carbohydrate Recognition	71:114	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	5	5	theme	residues	887:894	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	5	theme	residues	887:894	arg1	amide					914:918	protein backbone amide	897:918	protein backbone amide	897:918	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	5	theme	residues	887:894	arg1	water					930:934	buried water	923:934	buried water	923:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	5	theme	residues	887:894	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	3	6	theme	bridging	535:542	arg1	molecules					550:558	bridging water molecules	535:558	bridging water molecules	535:558	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	4	7	theme	energy	570:575	arg1	contribution					577:588	The free energy contribution	561:588	The free energy contribution of bridging and direct HBs	561:615	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	0	8	theme	Noncovalent	71:81	arg1	Recognition					104:114	Noncovalent Protein-Carbohydrate Recognition	71:114	Noncovalent Protein-Carbohydrate Recognition	71:114	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	5	9	with	HBs	839:841	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	9	with	HBs	839:841	arg1	amide					914:918	protein backbone amide	897:918	protein backbone amide	897:918	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	9	with	HBs	839:841	arg1	water					930:934	buried water	923:934	buried water	923:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	9	with	HBs	839:841	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	6	10	theme	hydroxy	1029:1035	arg1	groups					1037:1042	hydroxy groups	1029:1042	hydroxy groups	1029:1042	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	4	11	theme	free	565:568	arg1	contribution					577:588	The free energy contribution	561:588	The free energy contribution of bridging and direct HBs	561:615	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	6	12	dep	and	1147:1149	arg1	so					1151:1152	so	1151:1152	so	1151:1152	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	7	13	theme	site	1243:1246	arg1	replacement					1254:1264	binding site water replacement	1235:1264	binding site water replacement	1235:1264	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	2	14	theme	large-scale	285:295	arg1	analysis					297:304	a large-scale analysis	283:304	a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues	283:400	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	6	15	theme	solvent-exposed	994:1008	arg1	groups					1018:1023	all solvent-exposed hydroxy groups	990:1023	all solvent-exposed hydroxy groups	990:1023	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	1	16	theme	interest	181:188	arg1	interest					181:188	interest	181:188	interest	181:188	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	1	16	theme	interest	181:188	arg1	lot					174:176	a lot	172:176	a lot of interest due to their roles in various cellular processes and metabolism disorders	172:262	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	0	17	theme	Recognition	104:114	arg1	Determinants					55:66	Key Determinants	51:66	Key Determinants of Noncovalent Protein-Carbohydrate Recognition	51:114	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	17	theme	Recognition	104:114	arg1	Water					21:25	Water	21:25	Water	21:25	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	17	theme	Recognition	104:114	arg1	Bonding					40:46	Hydrogen Bonding	31:46	Hydrogen Bonding	31:46	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	5	18	theme	backbone	905:912	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	18	theme	backbone	905:912	arg1	amide					914:918	protein backbone amide	897:918	protein backbone amide	897:918	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	18	theme	backbone	905:912	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	6	19	theme	hydroxy	1010:1016	arg1	groups					1018:1023	all solvent-exposed hydroxy groups	990:1023	all solvent-exposed hydroxy groups	990:1023	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	3	20	theme	water	544:548	arg1	molecules					550:558	bridging water molecules	535:558	bridging water molecules	535:558	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	4	21	theme	energy	646:651	arg1	methodology					673:683	the free energy perturbation (FEP+) methodology	637:683	the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3	637:772	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	6	22	theme	ligand	1182:1187	arg1	potency					1189:1195	ligand potency	1182:1195	ligand potency	1182:1195	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	5	23	theme	Arg	875:877	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	3	24	theme	sugar	430:434	arg1	molecules					436:444	sugar molecules	430:444	sugar molecules	430:444	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	5	25	theme	hydroxy	797:803	arg1	groups					805:810	hydroxy groups	797:810	hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water	797:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	7	26	theme	binding	1273:1279	arg1	affinity					1281:1288	the binding affinity	1269:1288	the binding affinity to l-ABP	1269:1297	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	5	27	theme	Asp	870:872	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	2	28	theme	structures	317:326	arg1	analysis					297:304	a large-scale analysis	283:304	a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues	283:400	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	4	29	theme	HBs	613:615	arg1	contribution					577:588	The free energy contribution	561:588	The free energy contribution of bridging and direct HBs	561:615	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	6	30	theme	water	1093:1097	arg1	molecules					1099:1107	the solvent-exposed bridging water molecules	1064:1107	the solvent-exposed bridging water molecules	1064:1107	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	3	31	from	bonds	470:474	arg1	site					497:500	the binding site	485:500	the binding site	485:500	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	6	32	theme	binding	1130:1136	arg1	affinity					1138:1145	binding affinity	1130:1145	binding affinity	1130:1145	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	2	33	theme	protein	309:315	arg1	structures					317:326	protein structures	309:326	protein structures solved in complex with glucose, galactose and their substituted analogues	309:400	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	4	34	theme	direct	606:611	arg1	HBs					613:615	bridging and direct HBs	593:615	bridging and direct HBs	593:615	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	2	35	with	complex	338:344	arg1	glucose					351:357	glucose	351:357	glucose	351:357	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	2	35	with	complex	338:344	arg1	analogues					392:400	their substituted analogues	374:400	their substituted analogues	374:400	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	2	35	with	complex	338:344	arg1	galactose					360:368	galactose	360:368	galactose	360:368	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	4	36	theme	free	641:644	arg1	methodology					673:683	the free energy perturbation (FEP+) methodology	637:683	the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3	637:772	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	1	37	theme	various	212:218	arg1	processes					229:237	various cellular processes	212:237	various cellular processes	212:237	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	7	38	from	effect	1225:1230	arg1	affinity					1281:1288	the binding affinity	1269:1288	the binding affinity to l-ABP	1269:1297	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	1	39	theme	cellular	220:227	arg1	processes					229:237	various cellular processes	212:237	various cellular processes	212:237	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	5	40	theme	direct	832:837	arg1	HBs					839:841	direct HBs	832:841	direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water	832:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	41	theme	binding	959:965	arg1	affinity					967:974	binding affinity	959:974	binding affinity	959:974	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	42	theme	charged	852:858	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	42	theme	charged	852:858	arg1	amide					914:918	protein backbone amide	897:918	protein backbone amide	897:918	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	42	theme	charged	852:858	arg1	water					930:934	buried water	923:934	buried water	923:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	42	theme	charged	852:858	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	0	43	theme	Hydrogen	31:38	arg1	Determinants					55:66	Key Determinants	51:66	Key Determinants of Noncovalent Protein-Carbohydrate Recognition	51:114	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	43	theme	Hydrogen	31:38	arg1	Water					21:25	Water	21:25	Water	21:25	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	43	theme	Hydrogen	31:38	arg1	Bonding					40:46	Hydrogen Bonding	31:46	Hydrogen Bonding	31:46	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	6	44	with	HBs	1055:1057	arg1	molecules					1099:1107	the solvent-exposed bridging water molecules	1064:1107	the solvent-exposed bridging water molecules	1064:1107	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	1	45	from	roles	203:207	arg1	processes					229:237	various cellular processes	212:237	various cellular processes	212:237	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	1	45	from	roles	203:207	arg1	disorders					254:262	metabolism disorders	243:262	metabolism disorders	243:262	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	3	46	with	HBs	526:528	arg1	molecules					550:558	bridging water molecules	535:558	bridging water molecules	535:558	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	1	47	theme	protein-carbohydrate	131:150	arg1	recognition					152:162	protein-carbohydrate recognition	131:162	protein-carbohydrate recognition	131:162	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	6	48	theme	bridging	1084:1091	arg1	molecules					1099:1107	the solvent-exposed bridging water molecules	1064:1107	the solvent-exposed bridging water molecules	1064:1107	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	5	49	theme	protein	897:903	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	49	theme	protein	897:903	arg1	amide					914:918	protein backbone amide	897:918	protein backbone amide	897:918	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	49	theme	protein	897:903	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	1	50	theme	recognition	152:162	arg1	Mechanisms					117:126	Mechanisms	117:126	Mechanisms of protein-carbohydrate recognition	117:162	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	7	51	theme	water	1248:1252	arg1	replacement					1254:1264	binding site water replacement	1235:1264	binding site water replacement	1235:1264	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	3	52	dep	three	520:524	arg1	to					517:518	to	517:518	to	517:518	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	0	53	theme	Key	51:53	arg1	Determinants					55:66	Key Determinants	51:66	Key Determinants of Noncovalent Protein-Carbohydrate Recognition	51:114	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	53	theme	Key	51:53	arg1	Water					21:25	Water	21:25	Water	21:25	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	0	53	theme	Key	51:53	arg1	Bonding					40:46	Hydrogen Bonding	31:46	Hydrogen Bonding	31:46	The Role of Bridging Water and Hydrogen Bonding as Key Determinants of Noncovalent Protein-Carbohydrate Recognition.					
30380198	3	54	theme	hydrogen	461:468	arg1	HBs					477:479	HBs	477:479	HBs	477:479	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	3	54	theme	hydrogen	461:468	arg1	HBs					526:528	one to three HBs	513:528	one to three HBs with bridging water molecules	513:558	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	3	54	theme	hydrogen	461:468	arg1	bonds					470:474	five hydrogen bonds	456:474	five hydrogen bonds (HBs)	456:480	We found that, on average, sugar molecules establish five hydrogen bonds (HBs) in the binding site, including one to three HBs with bridging water molecules.					
30380198	5	55	theme	buried	923:928	arg1	groups					860:865	the charged groups	848:865	the charged groups of Asp, Arg and Glu residues	848:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	55	theme	buried	923:928	arg1	water					930:934	buried water	923:934	buried water	923:934	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	5	55	theme	buried	923:928	arg1	residues					887:894	Asp, Arg and Glu residues	870:894	Asp, Arg and Glu residues	870:894	We show that removing hydroxy groups that are engaged in direct HBs with the charged groups of Asp, Arg and Glu residues, protein backbone amide or buried water dramatically decreases binding affinity.					
30380198	7	56	theme	binding	1235:1241	arg1	replacement					1254:1264	binding site water replacement	1235:1264	binding site water replacement	1235:1264	Finally, we rationalize an effect of binding site water replacement on the binding affinity to l-ABP.					
30380198	2	57	theme	substituted	380:390	arg1	analogues					392:400	their substituted analogues	374:400	their substituted analogues	374:400	We have performed a large-scale analysis of protein structures solved in complex with glucose, galactose and their substituted analogues.					
30380198	1	58	theme	metabolism	243:252	arg1	disorders					254:262	metabolism disorders	243:262	metabolism disorders	243:262	Mechanisms of protein-carbohydrate recognition attract a lot of interest due to their roles in various cellular processes and metabolism disorders.					
30380198	6	59	theme	solvent-exposed	1068:1082	arg1	molecules					1099:1107	the solvent-exposed bridging water molecules	1064:1107	the solvent-exposed bridging water molecules	1064:1107	In contrast, all solvent-exposed hydroxy groups and hydroxy groups engaged in HBs with the solvent-exposed bridging water molecules contribute weakly to binding affinity and so can be replaced to optimize ligand potency.					
30380198	4	60	theme	perturbation	653:664	arg1	methodology					673:683	the free energy perturbation (FEP+) methodology	637:683	the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3	637:772	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
30380198	4	61	theme	bridging	593:600	arg1	HBs					613:615	bridging and direct HBs	593:615	bridging and direct HBs	593:615	The free energy contribution of bridging and direct HBs was estimated using the free energy perturbation (FEP+) methodology for mono- and disaccharides that bind to l-ABP, ttGBP, TrmB, hGalectin-1 and hGalectin-3.					
29700341	0	0	theme	Receptor	80:87	arg1	Preferences					97:107	C-type Lectin Receptor Binding Preferences	66:107	C-type Lectin Receptor Binding Preferences	66:107	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	1	1	theme	C-type	168:173	arg1	receptors					182:190	C-type lectin receptors	168:190	C-type lectin receptors	168:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	0	2	theme	Lectin	73:78	arg1	Preferences					97:107	C-type Lectin Receptor Binding Preferences	66:107	C-type Lectin Receptor Binding Preferences	66:107	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	1	3	theme	lectin	175:180	arg1	receptors					182:190	C-type lectin receptors	168:190	C-type lectin receptors	168:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	1	4	theme	blood	284:288	arg1	antigens					296:303	blood group antigens	284:303	blood group antigens as molecular mimicry to evade immune responses	284:350	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	1	5	theme	group	290:294	arg1	antigens					296:303	blood group antigens	284:303	blood group antigens as molecular mimicry to evade immune responses	284:350	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	0	6	theme	Binding	89:95	arg1	Preferences					97:107	C-type Lectin Receptor Binding Preferences	66:107	C-type Lectin Receptor Binding Preferences	66:107	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	2	7	theme	disaccharides	427:439	arg1	synthesis					375:383	the synthesis	371:383	the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray	371:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	3	8	theme	binding	557:563	arg1	preferences					565:575	binding preferences	557:575	binding preferences	557:575	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	2	9	theme	active	412:417	arg1	tri					419:421	ABO blood group antigen active tri	388:421	ABO blood group antigen active tri	388:421	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	2	10	theme	binding	460:466	arg1	specificity					468:478	the binding specificity	456:478	the binding specificity with various C-type lectin receptors using glycan microarray	456:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	2	11	theme	antigen	404:410	arg1	tri					419:421	ABO blood group antigen active tri	388:421	ABO blood group antigen active tri	388:421	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	2	12	theme	group	398:402	arg1	tri					419:421	ABO blood group antigen active tri	388:421	ABO blood group antigen active tri	388:421	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	0	13	theme	ABO	0:2	arg1	Antigens					4:11	ABO Antigens Active Tri- and Disaccharides	0:41	ABO Antigens Active Tri- and Disaccharides	0:41	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	2	14	theme	blood	392:396	arg1	tri					419:421	ABO blood group antigen active tri	388:421	ABO blood group antigen active tri	388:421	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	1	15	theme	molecular	308:316	arg1	mimicry					318:324	molecular mimicry	308:324	molecular mimicry to evade immune responses	308:350	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	3	16	theme	distinct	587:594	arg1	glycosylation					596:608	distinct glycosylation	587:608	distinct glycosylation on the galactose and fucose motifs	587:643	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	0	17	theme	Active	13:18	arg1	Tri-					20:23	Active Tri-	13:23	Active Tri-	13:23	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	1	18	theme	immune	221:226	arg1	responses					228:236	immune responses	221:236	immune responses	221:236	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	2	19	theme	lectin	500:505	arg1	receptors					507:515	various C-type lectin receptors	485:515	various C-type lectin receptors using glycan microarray	485:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	3	20	theme	fucose	631:636	arg1	motifs					638:643	the galactose and fucose motifs	613:643	motifs	638:643	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	2	21	theme	C-type	493:498	arg1	receptors					507:515	various C-type lectin receptors	485:515	various C-type lectin receptors using glycan microarray	485:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	3	22	theme	lectin	664:669	arg1	binding					680:686	C-type lectin receptor binding	657:686	C-type lectin receptor binding	657:686	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	2	23	theme	tri	419:421	arg1	synthesis					375:383	the synthesis	371:383	the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray	371:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	2	24	theme	various	485:491	arg1	receptors					507:515	various C-type lectin receptors	485:515	various C-type lectin receptors using glycan microarray	485:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	1	25	theme	immune	335:340	arg1	responses					342:350	immune responses	335:350	immune responses	335:350	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	3	26	theme	receptor	671:678	arg1	binding					680:686	C-type lectin receptor binding	657:686	C-type lectin receptor binding	657:686	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	3	27	theme	galactose	617:625	arg1	motifs					638:643	the galactose and fucose motifs	613:643	motifs	638:643	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	1	28	theme	blood	124:128	arg1	preferences					152:162	blood group antigen binding preferences	124:162	blood group antigen binding preferences for C-type lectin receptors	124:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	2	29	with	specificity	468:478	arg1	receptors					507:515	various C-type lectin receptors	485:515	various C-type lectin receptors using glycan microarray	485:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	1	30	theme	group	130:134	arg1	preferences					152:162	blood group antigen binding preferences	124:162	blood group antigen binding preferences for C-type lectin receptors	124:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	1	31	theme	antigen	136:142	arg1	preferences					152:162	blood group antigen binding preferences	124:162	blood group antigen binding preferences for C-type lectin receptors	124:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	3	32	gly	glycosylation	596:608	arg1	motifs					638:643	the galactose and fucose motifs	613:643	motifs	638:643	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	3	33	theme	preferences	565:575	arg1	results					546:552	The results	542:552	The results of binding preferences	542:575	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	3	34	theme	C-type	657:662	arg1	binding					680:686	C-type lectin receptor binding	657:686	C-type lectin receptor binding	657:686	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	1	35	theme	binding	144:150	arg1	preferences					152:162	blood group antigen binding preferences	124:162	blood group antigen binding preferences for C-type lectin receptors	124:190	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	1	36	theme	several	245:251	arg1	bacteria					267:274	several Gram-negative bacteria	245:274	several Gram-negative bacteria	245:274	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	0	37	theme	C-type	66:71	arg1	Preferences					97:107	C-type Lectin Receptor Binding Preferences	66:107	C-type Lectin Receptor Binding Preferences	66:107	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	0	38	dep	Antigens	4:11	arg1	Disaccharides					29:41	Disaccharides	29:41	Disaccharides	29:41	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	0	38	dep	Antigens	4:11	arg1	Tri-					20:23	Active Tri-	13:23	Active Tri-	13:23	ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.					
29700341	2	39	theme	ABO	388:390	arg1	tri					419:421	ABO blood group antigen active tri	388:421	ABO blood group antigen active tri	388:421	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	3	40	from	glycosylation	596:608	arg1	motifs					638:643	the galactose and fucose motifs	613:643	motifs	638:643	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
29700341	1	41	theme	Gram-negative	253:265	arg1	bacteria					267:274	several Gram-negative bacteria	245:274	several Gram-negative bacteria	245:274	Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses.					
29700341	2	42	theme	glycan	523:528	arg1	microarray					530:539	glycan microarray	523:539	glycan microarray	523:539	Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray.					
29700341	3	43	theme	Ca2+-dependent	727:740	arg1	fashion					742:748	a Ca2+-dependent fashion	725:748	a Ca2+-dependent fashion	725:748	The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.					
31190665	0	0	theme	closest	88:94	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	0	theme	closest	88:94	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	2	1	dep	homologous	563:572	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	1	dep	homologous	563:572	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	1	dep	homologous	563:572	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	2	theme	amino	579:583	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	2	theme	amino	579:583	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	2	theme	amino	579:583	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	3	theme	Onchocerca	534:543	arg1	ochengi					545:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	4	theme	%	577:577	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	4	theme	%	577:577	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	4	theme	%	577:577	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	1	5	theme	glutathione	171:181	arg1	GSTs					197:200	GSTs	197:200	GSTs	197:200	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	1	5	theme	glutathione	171:181	arg1	transferases					183:194	glutathione transferases	171:194	glutathione transferases (GSTs)	171:201	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	2	6	with	homologous	563:572	arg1	GST					623:625	an immunodominant GST	605:625	an immunodominant GST	605:625	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	6	with	homologous	563:572	arg1	OvGST1b					698:704	OvGST1b	698:704	OvGST1b	698:704	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	6	with	homologous	563:572	arg1	candidate					649:657	potential vaccine candidate	631:657	potential vaccine candidate from the human parasite	631:681	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	6	with	homologous	563:572	arg1	O.					684:685	O.	684:685	O.	684:685	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	5	7	theme	Chromatography	1267:1280	arg1	/MS					1286:1288	Liquid Chromatography (LC)/MS	1260:1288	Liquid Chromatography (LC)/MS following a coupled-enzyme assay	1260:1321	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	2	8	theme	cattle-infective	499:514	arg1	ochengi					545:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	1	9	theme	worm	343:346	arg1	establishment					358:370	worm infection establishment	343:370	worm infection establishment	343:370	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	6	10	from	interactions	1453:1464	arg1	study					1481:1485	the study	1477:1485	the study	1477:1485	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	4	11	theme	N-glycans	1014:1022	arg1	combination					931:941	A combination	929:941	A combination of lectin-blotting	929:960	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	11	theme	N-glycans	1014:1022	arg1	analyses					989:996	mass spectrometry (MS) analyses	966:996	mass spectrometry (MS) analyses of the released N-glycans	966:1022	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	1	12	theme	endogenous	269:278	arg1	substrates					280:289	endogenous substrates	269:289	endogenous substrates	269:289	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	4	13	theme	oligommanose-type	1127:1143	arg1	glycans					1145:1151	hybrid- and larger oligommanose-type glycans	1108:1151	hybrid- and larger oligommanose-type glycans	1108:1151	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	1	14	theme	infection	348:356	arg1	establishment					358:370	worm infection establishment	343:370	worm infection establishment	343:370	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	6	15	dep	second	1341:1346	arg1	characterized					1361:1373	characterized	1361:1373	characterized glycosylated GST	1361:1390	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	6	15	dep	second	1341:1346	arg1	reported					1348:1355	reported	1348:1355	reported	1348:1355	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	2	16	theme	immunodominant	608:621	arg1	GST					623:625	an immunodominant GST	605:625	an immunodominant GST	605:625	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	6	17	theme	onchocerciasis	1496:1509	arg1	use					1470:1472	use	1470:1472	use in the study of human onchocerciasis	1470:1509	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	6	17	theme	onchocerciasis	1496:1509	arg1	interactions					1453:1464	host-parasite interactions	1439:1464	host-parasite interactions	1439:1464	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	3	18	theme	chromatography	779:792	arg1	approach					794:801	a two-step affinity chromatography approach	759:801	a two-step affinity chromatography approach	759:801	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	0	19	theme	human	112:116	arg1	parasite					134:141	the human river blindness parasite	108:141	the human river blindness parasite	108:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	1	20	contain	possess	163:169	arg2	substrates					280:289	endogenous substrates	269:289	endogenous substrates	269:289	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	1	20	contain	possess	163:169	arg1	nematodes					153:161	Filarial nematodes	144:161	Filarial nematodes	144:161	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	1	20	contain	possess	163:169	arg2	enzymes					215:221	ubiquitous enzymes	204:221	ubiquitous enzymes with the potential to detoxify xenobiotic	204:263	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	1	20	contain	possess	163:169	arg2	GSTs					197:200	GSTs	197:200	GSTs	197:200	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	1	20	contain	possess	163:169	arg2	transferases					183:194	glutathione transferases	171:194	glutathione transferases (GSTs)	171:201	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	4	21	contain	contained	1046:1054	arg1	OoGST1					1039:1044	OoGST1	1039:1044	OoGST1	1039:1044	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	21	contain	contained	1046:1054	arg2	OoGST1					1039:1044	OoGST1	1039:1044	OoGST1	1039:1044	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	21	contain	contained	1046:1054	arg2	glycans					1145:1151	hybrid- and larger oligommanose-type glycans	1108:1151	hybrid- and larger oligommanose-type glycans	1108:1151	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	21	contain	contained	1046:1054	arg1	proportion					1164:1173	a lower proportion	1156:1173	a lower proportion	1156:1173	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	21	contain	contained	1046:1054	arg2	structure					1088:1096	oligomannose Man5GlcNAc2 structure	1063:1096	oligomannose Man5GlcNAc2 structure	1063:1096	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	2	22	dep	O.	684:685	arg1	volvulus					687:694	O. volvulus	684:694	O. volvulus	684:694	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	3	23	theme	affinity	770:777	arg1	chromatography					779:792	a two-step affinity chromatography	759:792	a two-step affinity chromatography approach	759:801	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	4	24	theme	spectrometry	971:982	arg1	analyses					989:996	mass spectrometry (MS) analyses	966:996	mass spectrometry (MS) analyses of the released N-glycans	966:1022	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	2	25	theme	glycosylated	463:474	arg1	OoGST1					481:486	OoGST1	481:486	OoGST1	481:486	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	25	theme	glycosylated	463:474	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	25	theme	glycosylated	463:474	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	25	theme	glycosylated	463:474	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	5	26	theme	prostaglandin	1212:1224	arg1	synthase					1226:1233	prostaglandin synthase	1212:1233	prostaglandin synthase activity	1212:1242	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	4	27	dep	oligommanose-type	1127:1143	arg1	larger					1120:1125	larger	1120:1125	larger	1120:1125	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	2	28	theme	class	457:461	arg1	OoGST1					481:486	OoGST1	481:486	OoGST1	481:486	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	28	theme	class	457:461	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	28	theme	class	457:461	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	28	theme	class	457:461	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	3	29	theme	enzymic	852:858	arg1	deglycosylation					860:874	enzymic deglycosylation	852:874	enzymic deglycosylation revealing the existence of at least four glycoforms	852:926	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	2	30	theme	acid	585:588	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	30	theme	acid	585:588	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	30	theme	acid	585:588	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	31	theme	vaccine	641:647	arg1	OvGST1b					698:704	OvGST1b	698:704	OvGST1b	698:704	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	31	theme	vaccine	641:647	arg1	candidate					649:657	potential vaccine candidate	631:657	potential vaccine candidate from the human parasite	631:681	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	31	theme	vaccine	641:647	arg1	O.					684:685	O.	684:685	O.	684:685	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	4	32	theme	Man5GlcNAc2	1076:1086	arg1	structure					1088:1096	oligomannose Man5GlcNAc2 structure	1063:1096	oligomannose Man5GlcNAc2 structure	1063:1096	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	2	33	from	parasite	674:681	arg1	GST					623:625	an immunodominant GST	605:625	an immunodominant GST	605:625	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	33	from	parasite	674:681	arg1	OvGST1b					698:704	OvGST1b	698:704	OvGST1b	698:704	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	33	from	parasite	674:681	arg1	candidate					649:657	potential vaccine candidate	631:657	potential vaccine candidate from the human parasite	631:681	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	33	from	parasite	674:681	arg1	O.					684:685	O.	684:685	O.	684:685	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	0	34	theme	novel	22:26	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	34	theme	novel	22:26	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	3	35	theme	Onchocerca	708:717	arg1	GSTs					734:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	0	36	theme	blindness	124:132	arg1	parasite					134:141	the human river blindness parasite	108:141	the human river blindness parasite	108:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	2	37	theme	potential	631:639	arg1	OvGST1b					698:704	OvGST1b	698:704	OvGST1b	698:704	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	37	theme	potential	631:639	arg1	candidate					649:657	potential vaccine candidate	631:657	potential vaccine candidate from the human parasite	631:681	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	37	theme	potential	631:639	arg1	O.					684:685	O.	684:685	O.	684:685	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	4	38	theme	oligomannose	1063:1074	arg1	structure					1088:1096	oligomannose Man5GlcNAc2 structure	1063:1096	oligomannose Man5GlcNAc2 structure	1063:1096	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	0	39	theme	river	118:122	arg1	parasite					134:141	the human river blindness parasite	108:141	the human river blindness parasite	108:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	6	40	theme	human	1490:1494	arg1	onchocerciasis					1496:1509	human onchocerciasis	1490:1509	human onchocerciasis	1490:1509	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	4	41	theme	lower	1158:1162	arg1	proportion					1164:1173	a lower proportion	1156:1173	a lower proportion	1156:1173	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	1	42	theme	ubiquitous	204:213	arg1	enzymes					215:221	ubiquitous enzymes	204:221	ubiquitous enzymes with the potential to detoxify xenobiotic	204:263	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	0	43	theme	glutathione	41:51	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	43	theme	glutathione	41:51	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	2	44	theme	nematode	525:532	arg1	ochengi					545:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	3	45	theme	1D	823:824	arg1	SDS-PAGE					826:833	2D and 1D SDS-PAGE	816:833	SDS-PAGE	826:833	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	6	46	from	role	1431:1434	arg1	use					1470:1472	use	1470:1472	use in the study of human onchocerciasis	1470:1509	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	6	46	from	role	1431:1434	arg1	interactions					1453:1464	host-parasite interactions	1439:1464	host-parasite interactions	1439:1464	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	5	47	theme	coupled-enzyme	1302:1315	arg1	assay					1317:1321	a coupled-enzyme assay	1300:1321	a coupled-enzyme assay	1300:1321	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	0	48	theme	glycosylated	28:39	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	48	theme	glycosylated	28:39	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	1	49	theme	host	309:312	arg1	system					321:326	the host immune system	305:326	the host immune system	305:326	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	0	50	theme	parasite	134:141	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	50	theme	parasite	134:141	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	2	51	gly	glycosylated	463:474	arg1	OoGST1					481:486	OoGST1	481:486	OoGST1	481:486	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	51	gly	glycosylated	463:474	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	51	gly	glycosylated	463:474	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	51	gly	glycosylated	463:474	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	52	theme	filarial	516:523	arg1	ochengi					545:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	the cattle-infective filarial nematode Onchocerca ochengi	495:551	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	53	theme	σ	455:455	arg1	OoGST1					481:486	OoGST1	481:486	OoGST1	481:486	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	53	theme	σ	455:455	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	53	theme	σ	455:455	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	53	theme	σ	455:455	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	1	54	theme	immune	314:319	arg1	system					321:326	the host immune system	305:326	the host immune system	305:326	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	6	55	theme	glycosylated	1375:1386	arg1	GST					1388:1390	glycosylated GST	1375:1390	glycosylated GST	1375:1390	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	1	56	with	substrates	280:289	arg1	potential					232:240	the potential to detoxify xenobiotic	228:263	the potential to detoxify xenobiotic	228:263	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	0	57	theme	transferase	53:63	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.	0:142	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	3	58	theme	ochengi	719:725	arg1	GSTs					734:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	1	59	with	enzymes	215:221	arg1	potential					232:240	the potential to detoxify xenobiotic	228:263	the potential to detoxify xenobiotic	228:263	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	2	60	theme	human	668:672	arg1	parasite					674:681	the human parasite	664:681	the human parasite	664:681	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	6	61	theme	host-parasite	1439:1451	arg1	interactions					1453:1464	host-parasite interactions	1439:1464	host-parasite interactions	1439:1464	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	3	62	theme	2D	816:817	arg1	SDS-PAGE					826:833	2D and 1D SDS-PAGE	816:833	SDS-PAGE	826:833	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	3	63	theme	native	727:732	arg1	GSTs					734:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs	708:737	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	1	64	with	transferases	183:194	arg1	potential					232:240	the potential to detoxify xenobiotic	228:263	the potential to detoxify xenobiotic	228:263	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	6	65	gly	glycosylated	1375:1386	arg1	GST					1388:1390	glycosylated GST	1375:1390	glycosylated GST	1375:1390	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	0	66	gly	glycosylated	28:39	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	66	gly	glycosylated	28:39	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	6	67	theme	potential	1421:1429	arg1	role					1431:1434	its potential role	1417:1434	its potential role in host-parasite interactions and use in the study of human onchocerciasis	1417:1509	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	4	68	theme	mass	966:969	arg1	spectrometry					971:982	mass spectrometry	966:982	mass spectrometry (MS) analyses of the released N-glycans	966:1022	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	68	theme	mass	966:969	arg1	MS					985:986	MS	985:986	MS	985:986	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	3	69	theme	glycoforms	917:926	arg1	existence					890:898	the existence	886:898	the existence of at least four glycoforms	886:926	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	4	70	theme	hybrid-	1108:1114	arg1	glycans					1145:1151	hybrid- and larger oligommanose-type glycans	1108:1151	hybrid- and larger oligommanose-type glycans	1108:1151	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	3	71	theme	two-step	761:768	arg1	chromatography					779:792	a two-step affinity chromatography	759:792	a two-step affinity chromatography approach	759:801	Onchocerca ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms.					
31190665	0	72	theme	ochengi	79:85	arg1	transferase					53:63	a novel glycosylated glutathione transferase	20:63	a novel glycosylated glutathione transferase of Onchocerca ochengi	20:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	0	72	theme	ochengi	79:85	arg1	relative					96:103	closest relative	88:103	closest relative of the human river blindness parasite	88:141	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	5	73	theme	Liquid	1260:1265	arg1	LC					1283:1284	LC	1283:1284	LC	1283:1284	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	5	73	theme	Liquid	1260:1265	arg1	Chromatography					1267:1280	Liquid Chromatography	1260:1280	Liquid Chromatography (LC)/MS following a coupled-enzyme assay	1260:1321	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	6	74	from	use	1470:1472	arg1	study					1481:1485	the study	1477:1485	the study	1477:1485	This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.					
31190665	1	75	theme	Filarial	144:151	arg1	nematodes					153:161	Filarial nematodes	144:161	Filarial nematodes	144:161	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host.					
31190665	2	76	from	ochengi	545:551	arg1	OoGST1					481:486	OoGST1	481:486	OoGST1	481:486	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	76	from	ochengi	545:551	arg1	identity					590:597	99% amino acid identity	575:597	99% amino acid identity	575:597	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	76	from	ochengi	545:551	arg1	GST					476:478	a σ class glycosylated GST	453:478	a σ class glycosylated GST (OoGST1)	453:487	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	2	76	from	ochengi	545:551	arg1	homologous					563:572	homologous	563:572	homologous	563:572	Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b).					
31190665	0	77	theme	Onchocerca	68:77	arg1	ochengi					79:85	Onchocerca ochengi	68:85	Onchocerca ochengi	68:85	Characterization of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite.					
31190665	4	78	theme	lectin-blotting	946:960	arg1	combination					931:941	A combination	929:941	A combination of lectin-blotting	929:960	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	4	78	theme	lectin-blotting	946:960	arg1	analyses					989:996	mass spectrometry (MS) analyses	966:996	mass spectrometry (MS) analyses of the released N-glycans	966:1022	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
31190665	5	79	theme	purified	1189:1196	arg1	OoGST1					1198:1203	purified OoGST1	1189:1203	purified OoGST1	1189:1203	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	5	80	theme	synthase	1226:1233	arg1	activity					1235:1242	prostaglandin synthase activity	1212:1242	prostaglandin synthase activity	1212:1242	Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay.					
31190665	4	81	theme	released	1005:1012	arg1	N-glycans					1014:1022	the released N-glycans	1001:1022	the released N-glycans	1001:1022	A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion.					
30983324	4	0	theme	p	1017:1017	arg1	Ka					1019:1020	p Ka	1017:1020	p Ka	1017:1020	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	1	theme	efficient	1549:1557	arg1	assays					1524:1529	LC/MS/MS pairwise assays	1506:1529	LC/MS/MS pairwise assays	1506:1529	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	1	theme	efficient	1549:1557	arg1	PGC-SPE					1494:1500	stepwise PGC-SPE	1485:1500	stepwise PGC-SPE	1485:1500	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	1	theme	efficient	1549:1557	arg1	tool					1559:1562	an efficient tool	1546:1562	an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1546:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	2	2	theme	in-depth	363:370	arg1	characterization					381:396	comprehensive and in-depth glycomic characterization	345:396	characterization	381:396	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	0	3	theme	Glycans	90:96	arg1	Capturing					63:71	Selective Capturing	53:71	Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS	53:132	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	3	4	dep	extraction	795:804	arg1	LC/MS					832:836	LC/MS	832:836	LC/MS	832:836	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	1	5	theme	important	172:180	arg1	glycosylation					139:151	The glycosylation	135:151	The glycosylation of biologics	135:164	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	5	theme	important	172:180	arg1	factor					182:187	an important factor	169:187	an important factor in pharmacological functions such as efficacy, safety, and biological activity	169:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	3	6	dep	separation	688:697	arg1	the					684:686	the	684:686	the	684:686	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	3	7	theme	various	723:729	arg1	glycans					731:737	various glycans	723:737	various glycans of biotherapeutics	723:756	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	4	8	theme	graphitized	926:936	arg1	cartridge					951:959	a porous graphitized carbon (PGC) cartridge	917:959	a porous graphitized carbon (PGC) cartridge	917:959	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	9	gly	glycoproteins	1611:1623	arg1	glycoproteins					1611:1623	therapeutic glycoproteins	1599:1623	therapeutic glycoproteins	1599:1623	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	0	10	theme	Stepwise	104:111	arg1	PGC-SPE					113:119	PGC-SPE	113:119	PGC-SPE	113:119	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	4	11	theme	porous	919:924	arg1	cartridge					951:959	a porous graphitized carbon (PGC) cartridge	917:959	a porous graphitized carbon (PGC) cartridge	917:959	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	6	12	theme	agalsidase-beta	1213:1227	arg1	characterization					1193:1208	glycomic characterization	1184:1208	glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans	1184:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	8	13	theme	glycosylation	1574:1586	arg1	changes					1588:1594	glycosylation changes	1574:1594	glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1574:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	13	theme	glycosylation	1574:1586	arg1	species					1651:1657	abundant acidic species	1635:1657	abundant acidic species in biologics or biosimilar development	1635:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	2	14	theme	biological	401:410	arg1	products					412:419	biological products	401:419	biological products	401:419	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	7	15	theme	different	1393:1401	arg1	batches					1403:1409	different batches	1393:1409	different batches of enzymes	1393:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	3	16	theme	biotherapeutics	742:756	arg1	glycans					731:737	various glycans	723:737	various glycans of biotherapeutics	723:756	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	1	17	from	changes	301:307	arg1	environment					325:335	the cellular environment	312:335	the cellular environment	312:335	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	8	18	from	species	1651:1657	arg1	biologics					1662:1670	biologics	1662:1670	biologics	1662:1670	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	18	from	species	1651:1657	arg1	development					1686:1696	biosimilar development	1675:1696	biosimilar development	1675:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	19	theme	biosimilar	1675:1684	arg1	development					1686:1696	biosimilar development	1675:1696	biosimilar development	1675:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	2	20	theme	products	412:419	arg1	characterization					381:396	comprehensive and in-depth glycomic characterization	345:396	characterization	381:396	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	8	21	theme	abundant	1635:1642	arg1	species					1651:1657	abundant acidic species	1635:1657	abundant acidic species in biologics or biosimilar development	1635:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	6	22	contain	containing	1266:1275	arg1	enzyme					1259:1264	a representative therapeutic enzyme	1230:1264	a representative therapeutic enzyme containing both phosphorylated and sialylated glycans	1230:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	22	contain	containing	1266:1275	arg1	agalsidase-beta					1213:1227	agalsidase-beta	1213:1227	agalsidase-beta	1213:1227	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	22	contain	containing	1266:1275	arg2	glycans					1312:1318	both phosphorylated and sialylated glycans	1277:1318	both phosphorylated and sialylated glycans	1277:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	23	theme	glycomic	1184:1191	arg1	characterization					1193:1208	glycomic characterization	1184:1208	glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans	1184:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	4	24	theme	carbon	938:943	arg1	cartridge					951:959	a porous graphitized carbon (PGC) cartridge	917:959	a porous graphitized carbon (PGC) cartridge	917:959	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	1	25	theme	pharmacological	192:206	arg1	safety					236:241	safety	236:241	safety	236:241	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	25	theme	pharmacological	192:206	arg1	functions					208:216	pharmacological functions	192:216	pharmacological functions such as efficacy, safety, and biological activity	192:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	25	theme	pharmacological	192:206	arg1	efficacy					226:233	efficacy	226:233	efficacy	226:233	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	25	theme	pharmacological	192:206	arg1	activity					259:266	biological activity	248:266	biological activity	248:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	0	26	theme	Comprehensive	0:12	arg1	Characterization					14:29	Comprehensive Characterization	0:29	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.	0:133	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	3	27	theme	extraction	795:804	arg1	combination					768:778	the combination	764:778	the combination of solid-phase extraction (SPE) and high resolution LC/MS	764:836	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	6	28	theme	sialylated	1301:1310	arg1	glycans					1312:1318	both phosphorylated and sialylated glycans	1277:1318	both phosphorylated and sialylated glycans	1277:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	0	29	theme	Biotherapeutics	34:48	arg1	Characterization					14:29	Comprehensive Characterization	0:29	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.	0:133	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	6	30	theme	representative	1232:1245	arg1	enzyme					1259:1264	a representative therapeutic enzyme	1230:1264	a representative therapeutic enzyme containing both phosphorylated and sialylated glycans	1230:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	30	theme	representative	1232:1245	arg1	agalsidase-beta					1213:1227	agalsidase-beta	1213:1227	agalsidase-beta	1213:1227	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	5	31	theme	LC-MS	1035:1039	arg1	analyses					1052:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses enabled us to obtain glycan compositions, structures, and quantitative information.					
30983324	7	32	theme	functional	1360:1369	arg1	glycans					1371:1377	functional glycans	1360:1377	functional glycans released from different batches of enzymes	1360:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	2	33	theme	product	451:457	arg1	quality					459:465	product quality	451:465	product quality	451:465	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	1	34	theme	subtle	294:299	arg1	changes					301:307	subtle changes	294:307	subtle changes in the cellular environment	294:335	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	2	35	theme	glycan	563:568	arg1	microheterogeneity					570:587	glycan microheterogeneity	563:587	glycan microheterogeneity produced by enzymatic machinery	563:619	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	5	36	theme	glycan	1082:1087	arg1	compositions					1089:1100	glycan compositions	1082:1100	glycan compositions	1082:1100	Subsequent LC-MS and -MS/MS analyses enabled us to obtain glycan compositions, structures, and quantitative information.					
30983324	8	37	theme	acidic	1644:1649	arg1	species					1651:1657	abundant acidic species	1635:1657	abundant acidic species in biologics or biosimilar development	1635:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	7	38	theme	glycans	1371:1377	arg1	analysis					1348:1355	a comparative analysis	1334:1355	a comparative analysis of functional glycans released from different batches of enzymes	1334:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	2	39	theme	regulatory	500:509	arg1	approval					511:518	regulatory approval	500:518	regulatory approval	500:518	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	3	40	theme	solid-phase	783:793	arg1	extraction					795:804	solid-phase extraction	783:804	solid-phase extraction (SPE)	783:810	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	3	40	theme	solid-phase	783:793	arg1	SPE					807:809	SPE	807:809	SPE	807:809	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	4	41	theme	molecule	980:987	arg1	size					989:992	molecule size	980:992	molecule size	980:992	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	42	theme	LC/MS/MS	1506:1513	arg1	assays					1524:1529	LC/MS/MS pairwise assays	1506:1529	LC/MS/MS pairwise assays	1506:1529	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	42	theme	LC/MS/MS	1506:1513	arg1	PGC-SPE					1494:1500	stepwise PGC-SPE	1485:1500	stepwise PGC-SPE	1485:1500	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	42	theme	LC/MS/MS	1506:1513	arg1	tool					1559:1562	an efficient tool	1546:1562	an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1546:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	4	43	theme	multiple-acidic	851:865	arg1	glycans					867:873	Neutral and multiple-acidic glycans	839:873	Neutral and multiple-acidic glycans	839:873	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	1	44	gly	glycosylation	139:151	arg1	biologics					156:164	biologics	156:164	biologics	156:164	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	6	45	theme	phosphorylated	1282:1295	arg1	glycans					1312:1318	both phosphorylated and sialylated glycans	1277:1318	both phosphorylated and sialylated glycans	1277:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	8	46	used	used	1538:1541	arg2	PGC-SPE					1494:1500	stepwise PGC-SPE	1485:1500	stepwise PGC-SPE	1485:1500	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	46	used	used	1538:1541	arg2	tool					1559:1562	an efficient tool	1546:1562	an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1546:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	46	used	used	1538:1541	arg2	assays					1524:1529	LC/MS/MS pairwise assays	1506:1529	LC/MS/MS pairwise assays	1506:1529	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	2	47	theme	prior	491:495	arg1	consistency					479:489	consistency	479:489	consistency prior to regulatory approval	479:518	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	3	48	theme	high	816:819	arg1	resolution					821:830	high resolution	816:830	high resolution	816:830	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	3	49	theme	systematic	657:666	arg1	methodology					668:678	a systematic methodology	655:678	a systematic methodology for the separation and characterization of various glycans of biotherapeutics	655:756	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	5	50	theme	-MS/MS	1045:1050	arg1	analyses					1052:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses enabled us to obtain glycan compositions, structures, and quantitative information.					
30983324	5	51	theme	quantitative	1119:1130	arg1	information					1132:1142	quantitative information	1119:1142	quantitative information	1119:1142	Subsequent LC-MS and -MS/MS analyses enabled us to obtain glycan compositions, structures, and quantitative information.					
30983324	2	52	theme	comprehensive	345:357	arg1	characterization					381:396	comprehensive and in-depth glycomic characterization	345:396	characterization	381:396	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	8	53	theme	therapeutic	1599:1609	arg1	glycoproteins					1611:1623	therapeutic glycoproteins	1599:1623	therapeutic glycoproteins	1599:1623	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	1	54	theme	cellular	316:323	arg1	environment					325:335	the cellular environment	312:335	the cellular environment	312:335	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	2	55	theme	enzymatic	601:609	arg1	machinery					611:619	enzymatic machinery	601:619	enzymatic machinery	601:619	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	7	56	theme	comparative	1336:1346	arg1	analysis					1348:1355	a comparative analysis	1334:1355	a comparative analysis of functional glycans released from different batches of enzymes	1334:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	7	57	theme	enzymes	1414:1420	arg1	batches					1403:1409	different batches	1393:1409	different batches of enzymes	1393:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	4	58	dep	acidity	1008:1014	arg1	Ka					1019:1020	p Ka	1017:1020	p Ka	1017:1020	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	59	theme	stepwise	1485:1492	arg1	assays					1524:1529	LC/MS/MS pairwise assays	1506:1529	LC/MS/MS pairwise assays	1506:1529	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	59	theme	stepwise	1485:1492	arg1	PGC-SPE					1494:1500	stepwise PGC-SPE	1485:1500	stepwise PGC-SPE	1485:1500	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	59	theme	stepwise	1485:1492	arg1	tool					1559:1562	an efficient tool	1546:1562	an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1546:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	0	60	theme	Selective	53:61	arg1	Capturing					63:71	Selective Capturing	53:71	Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS	53:132	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	4	61	theme	PGC	946:948	arg1	cartridge					951:959	a porous graphitized carbon (PGC) cartridge	917:959	a porous graphitized carbon (PGC) cartridge	917:959	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	62	theme	pairwise	1515:1522	arg1	assays					1524:1529	LC/MS/MS pairwise assays	1506:1529	LC/MS/MS pairwise assays	1506:1529	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	62	theme	pairwise	1515:1522	arg1	PGC-SPE					1494:1500	stepwise PGC-SPE	1485:1500	stepwise PGC-SPE	1485:1500	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	62	theme	pairwise	1515:1522	arg1	tool					1559:1562	an efficient tool	1546:1562	an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1546:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	6	63	theme	therapeutic	1247:1257	arg1	enzyme					1259:1264	a representative therapeutic enzyme	1230:1264	a representative therapeutic enzyme containing both phosphorylated and sialylated glycans	1230:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	63	theme	therapeutic	1247:1257	arg1	agalsidase-beta					1213:1227	agalsidase-beta	1213:1227	agalsidase-beta	1213:1227	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	6	64	gly	sialylated	1301:1310	arg1	glycans					1312:1318	both phosphorylated and sialylated glycans	1277:1318	both phosphorylated and sialylated glycans	1277:1318	Indeed, we have successfully performed glycomic characterization of agalsidase-beta, a representative therapeutic enzyme containing both phosphorylated and sialylated glycans.					
30983324	0	65	theme	Acidic	83:88	arg1	Glycans					90:96	Highly Acidic Glycans	76:96	Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS	76:132	Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans Using Stepwise PGC-SPE and LC/MS/MS.					
30983324	7	66	attach	released	1379:1386	arg2	glycans					1371:1377	functional glycans	1360:1377	functional glycans released from different batches of enzymes	1360:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	7	66	attach	released	1379:1386	arg1	batches					1403:1409	different batches	1393:1409	different batches of enzymes	1393:1420	In addition, a comparative analysis of functional glycans released from different batches of enzymes was performed to verify our method.					
30983324	4	67	theme	Neutral	839:845	arg1	glycans					867:873	Neutral and multiple-acidic glycans	839:873	Neutral and multiple-acidic glycans	839:873	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	8	68	theme	glycoproteins	1611:1623	arg1	changes					1588:1594	glycosylation changes	1574:1594	glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development	1574:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	8	68	theme	glycoproteins	1611:1623	arg1	species					1651:1657	abundant acidic species	1635:1657	abundant acidic species in biologics or biosimilar development	1635:1696	These results suggest that stepwise PGC-SPE and LC/MS/MS pairwise assays can be used as an efficient tool to detect glycosylation changes of therapeutic glycoproteins including abundant acidic species in biologics or biosimilar development.					
30983324	1	69	theme	biologics	156:164	arg1	glycosylation					139:151	The glycosylation	135:151	The glycosylation of biologics	135:164	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	69	theme	biologics	156:164	arg1	factor					182:187	an important factor	169:187	an important factor in pharmacological functions such as efficacy, safety, and biological activity	169:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	3	70	theme	resolution	821:830	arg1	combination					768:778	the combination	764:778	the combination of solid-phase extraction (SPE) and high resolution LC/MS	764:836	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	3	71	theme	glycans	731:737	arg1	separation					688:697	separation	688:697	separation	688:697	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	3	71	theme	glycans	731:737	arg1	characterization					703:718	characterization	703:718	characterization	703:718	In this study, we have developed a systematic methodology for the separation and characterization of various glycans of biotherapeutics using the combination of solid-phase extraction (SPE) and high resolution LC/MS.					
30983324	5	72	theme	Subsequent	1024:1033	arg1	analyses					1052:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses	1024:1059	Subsequent LC-MS and -MS/MS analyses enabled us to obtain glycan compositions, structures, and quantitative information.					
30983324	1	73	from	factor	182:187	arg1	safety					236:241	safety	236:241	safety	236:241	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	73	from	factor	182:187	arg1	functions					208:216	pharmacological functions	192:216	pharmacological functions such as efficacy, safety, and biological activity	192:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	73	from	factor	182:187	arg1	efficacy					226:233	efficacy	226:233	efficacy	226:233	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	1	73	from	factor	182:187	arg1	activity					259:266	biological activity	248:266	biological activity	248:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30983324	2	74	theme	glycomic	372:379	arg1	characterization					381:396	comprehensive and in-depth glycomic characterization	345:396	characterization	381:396	Hence, comprehensive and in-depth glycomic characterization of biological products should be performed to ensure product quality and process consistency prior to regulatory approval, but it is still highly challenging due to glycan microheterogeneity produced by enzymatic machinery.					
30983324	4	75	with	SPE	908:910	arg1	cartridge					951:959	a porous graphitized carbon (PGC) cartridge	917:959	a porous graphitized carbon (PGC) cartridge	917:959	Neutral and multiple-acidic glycans were selectively fractionated by SPE with a porous graphitized carbon (PGC) cartridge according to their molecule size and polarity (acidity, p Ka).					
30983324	1	76	theme	biological	248:257	arg1	activity					259:266	biological activity	248:266	biological activity	248:266	The glycosylation of biologics is an important factor in pharmacological functions such as efficacy, safety, and biological activity and is easily affected by subtle changes in the cellular environment.					
30990348	2	0	dep	isomers	704:710	arg1	isomers					704:710	the G1F isomers	696:710	the G1F isomers (G1aF and G1bF)	696:726	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	0	dep	isomers	704:710	arg1	G1bF					722:725	G1bF	722:725	G1bF	722:725	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	0	dep	isomers	704:710	arg1	G1aF					713:716	G1aF	713:716	G1aF	713:716	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	9	1	theme	HILIC	2327:2331	arg1	chromatography					2365:2378	HILIC, hydrophilic interaction liquid chromatography	2327:2378	chromatography	2365:2378	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	2	2	theme	Fc-glycans	526:535	arg1	Fc-glycans					526:535	Fc-glycans	526:535	Fc-glycans	526:535	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	2	theme	Fc-glycans	526:535	arg1	residues					514:521	Terminal galactose (Gal) residues	489:521	Terminal galactose (Gal) residues of Fc-glycans	489:535	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	7	3	theme	Man	1610:1612	arg1	Fc-glycans					1626:1635	Fc-glycans	1626:1635	Fc-glycans	1626:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	3	theme	Man	1610:1612	arg1	arm					1619:1621	the Man α1-6 arm	1606:1621	the Man α1-6 arm of Fc-glycans	1606:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	9	4	theme	phosphate-buffered	2559:2576	arg1	antibody					2507:2514	monoclonal antibody	2496:2514	monoclonal antibody	2496:2514	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	4	theme	phosphate-buffered	2559:2576	arg1	saline					2578:2583	phosphate-buffered saline	2559:2583	phosphate-buffered saline	2559:2583	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	5	gly	core-fucosylated	256:271	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	5	gly	core-fucosylated	256:271	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	9	6	theme	hydrophilic	2334:2344	arg1	chromatography					2365:2378	HILIC, hydrophilic interaction liquid chromatography	2327:2378	chromatography	2365:2378	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	3	7	theme	G1aF	894:897	arg1	mAb					899:901	G1aF mAb	894:901	G1aF mAb	894:901	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	2	8	theme	isomers	704:710	arg1	impact					686:691	the impact	682:691	the impact of the G1F isomers (G1aF and G1bF) on the effector functions	682:752	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	9	9	theme	cell-mediated	1919:1931	arg1	cytotoxicity					1933:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	10	theme	liquid	2358:2363	arg1	chromatography					2365:2378	HILIC, hydrophilic interaction liquid chromatography	2327:2378	chromatography	2365:2378	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	7	11	theme	Gal	1591:1593	arg1	residue					1595:1601	the terminal Gal residue	1578:1601	the terminal Gal residue on the Man α1-6 arm of Fc-glycans	1578:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	4	12	theme	G1aF	999:1002	arg1	mAb					1004:1006	G1aF mAb	999:1006	G1aF mAb	999:1006	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	3	13	dep	G0F	880:882	arg1	mAb					899:901	G1aF mAb	894:901	G1aF mAb	894:901	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	3	13	dep	G0F	880:882	arg1	mAb					909:911	G1bF mAb	904:911	G1bF mAb	904:911	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	3	13	dep	G0F	880:882	arg1	mAb					921:923	G0F mAb	917:923	G0F mAb	917:923	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	3	13	dep	G0F	880:882	arg1	mAb					889:891	G2F mAb	885:891	G2F mAb	885:891	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	2	14	theme	galactose	498:506	arg1	Fc-glycans					526:535	Fc-glycans	526:535	Fc-glycans	526:535	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	14	theme	galactose	498:506	arg1	residues					514:521	Terminal galactose (Gal) residues	489:521	Terminal galactose (Gal) residues of Fc-glycans	489:535	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	0	15	theme	therapeutic	101:111	arg1	antibodies					124:133	therapeutic monoclonal antibodies	101:133	therapeutic monoclonal antibodies	101:133	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	1	16	theme	terminal	358:365	arg1	galactosylation					367:381	terminal galactosylation	358:381	terminal galactosylation on the Man α1-6 arm	358:401	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	9	17	theme	component	1963:1971	arg1	1q					1973:1974	component 1q	1963:1974	component 1q	1963:1974	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	18	theme	antibodies	124:133	arg1	functions					88:96	the effector functions	75:96	the effector functions of therapeutic monoclonal antibodies	75:133	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	6	19	theme	structures	1281:1290	arg1	analysis					1261:1268	Hydrogen-deuterium exchange/mass spectrometry analysis	1215:1268	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs	1215:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	9	20	theme	balance-solid-phase	2413:2431	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	20	theme	balance-solid-phase	2413:2431	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	21	from	arm	55:57	arg1	Effects					0:6	Effects	0:6	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.	0:134	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	4	22	theme	FcγR-binding	1031:1042	arg1	activities					1044:1053	FcγR-binding activities	1031:1053	FcγR-binding activities	1031:1053	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	1	23	theme	Man	390:392	arg1	arm					399:401	the Man α1-6 arm	386:401	the Man α1-6 arm	386:401	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	24	theme	domain	1420:1425	arg1	stability					1399:1407	the structural stability	1384:1407	the structural stability of the CH2 domain	1384:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	25	theme	CH2	1564:1566	arg1	domain					1568:1573	the CH2 domain	1560:1573	the CH2 domain	1560:1573	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	5	26	theme	mAb	1137:1139	arg1	activities					1118:1127	The activities	1114:1127	The activities of G1aF mAb and G1bF mAb	1114:1152	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	5	27	theme	G1bF	1145:1148	arg1	mAb					1150:1152	G1bF mAb	1145:1152	G1bF mAb	1145:1152	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	6	28	theme	dynamic	1273:1279	arg1	structures					1281:1290	dynamic structures	1273:1290	dynamic structures of mAbs	1273:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	1	29	theme	crystallizable	144:157	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	29	theme	crystallizable	144:157	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	7	30	theme	structural	1532:1541	arg1	stabilization					1543:1555	the structural stabilization	1528:1555	the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans	1528:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	30	theme	structural	1532:1541	arg1	important					1644:1652	important	1644:1652	important	1644:1652	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	9	31	dep	ABC	1839:1841	arg1	attribute					2039:2047	critical quality attribute	2022:2047	critical quality attribute	2022:2047	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	Fuc					2155:2157	Fuc	2155:2157	Fuc	2155:2157	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	Fc					2105:2106	Fc	2105:2106	Fc	2105:2106	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	S-transferase					2230:2242	glutathione S-transferase	2218:2242	glutathione S-transferase	2218:2242	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	exchange					2317:2324	hydrogen-deuterium exchange	2298:2324	hydrogen-deuterium exchange	2298:2324	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	fragment					2124:2131	crystallizable fragment	2109:2131	crystallizable fragment	2109:2131	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	acetonitrile					1880:1891	acetonitrile	1880:1891	acetonitrile	1880:1891	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	CDC					1977:1979	CDC	1977:1979	CDC	1977:1979	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	solution					1865:1872	ammonium bicarbonate solution	1844:1872	ammonium bicarbonate solution	1844:1872	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	complement					1952:1961	complement	1952:1961	complement	1952:1961	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	FA					2088:2089	FA	2088:2089	FA	2088:2089	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	cytotoxicity					2003:2014	complement-dependent cytotoxicity	1982:2014	complement-dependent cytotoxicity	1982:2014	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	galactose					2173:2181	galactose	2173:2181	galactose	2173:2181	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	Endo					2050:2053	Endo	2050:2053	Endo	2050:2053	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	acid					2099:2102	formic acid	2092:2102	formic acid	2092:2102	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	fucose					2160:2165	fucose	2160:2165	fucose	2160:2165	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	receptors					2144:2152	FcγR, Fcγ receptors	2134:2152	FcγR, Fcγ receptors	2134:2152	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	cytotoxicity					1933:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	1q					1973:1974	component 1q	1963:1974	component 1q	1963:1974	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	CQA					2017:2019	CQA	2017:2019	CQA	2017:2019	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	GlcNAc					2184:2189	GlcNAc	2184:2189	GlcNAc	2184:2189	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	ADCC					1894:1897	ADCC	1894:1897	ADCC	1894:1897	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	C1q					1947:1949	C1q	1947:1949	C1q	1947:1949	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	endo-β-N-acetylglucosaminidase					2056:2085	endo-β-N-acetylglucosaminidase	2056:2085	endo-β-N-acetylglucosaminidase	2056:2085	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	Gal					2168:2170	Gal	2168:2170	Gal	2168:2170	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	receptor					2281:2288	human epidermal growth factor receptor 2	2251:2290	human epidermal growth factor receptor 2	2251:2290	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	31	dep	ABC	1839:1841	arg1	ACN					1875:1877	ACN	1875:1877	ACN	1875:1877	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	32	dep	sialylglycopeptides	2604:2622	arg1	ABC					1839:1841	ABC	1839:1841	ABC	1839:1841	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	33	theme	Fc-glycan	62:70	arg1	Fc-glycan					62:70	Fc-glycan	62:70	Fc-glycan	62:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	33	theme	Fc-glycan	62:70	arg1	arm					55:57	mannose α1-6 arm	42:57	mannose α1-6 arm of Fc-glycan	42:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	1	34	theme	full	329:332	arg1	G2F					324:326	G2F	324:326	G2F	324:326	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	34	theme	full	329:332	arg1	galactosylation					334:348	full galactosylation	329:348	full galactosylation	329:348	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	0	35	from	Effects	0:6	arg1	Fc-glycan					62:70	Fc-glycan	62:70	Fc-glycan	62:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	35	from	Effects	0:6	arg1	arm					55:57	mannose α1-6 arm	42:57	mannose α1-6 arm of Fc-glycan	42:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	35	from	Effects	0:6	arg1	functions					88:96	the effector functions	75:96	the effector functions of therapeutic monoclonal antibodies	75:133	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	2	36	theme	antibody-dependent	587:604	arg1	cytotoxicity					620:631	antibody-dependent cell-mediated cytotoxicity	587:631	antibody-dependent cell-mediated cytotoxicity	587:631	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	9	37	theme	critical	2022:2029	arg1	attribute					2039:2047	critical quality attribute	2022:2047	critical quality attribute	2022:2047	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	8	38	theme	isomers	1759:1765	arg1	impact					1745:1750	the impact	1741:1750	the impact of G1F isomers on the effector functions and dynamic structure of mAbs	1741:1821	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	9	39	theme	glutathione	2218:2228	arg1	S-transferase					2230:2242	glutathione S-transferase	2218:2242	glutathione S-transferase	2218:2242	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	39	theme	glutathione	2218:2228	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	3	40	theme	mAbs	835:838	arg1	types					800:804	four types	795:804	four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively)	795:938	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	4	41	theme	G1bF	1104:1107	arg1	mAb					1109:1111	G1bF mAb	1104:1111	G1bF mAb	1104:1111	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	1	42	from	galactosylation	367:381	arg1	arm					399:401	the Man α1-6 arm	386:401	the Man α1-6 arm	386:401	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	3	43	theme	glycoengineered	809:823	arg1	mAbs					835:838	glycoengineered anti-CD20 mAbs	809:838	glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively)	809:938	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	0	44	from	residues	30:37	arg1	Fc-glycan					62:70	Fc-glycan	62:70	Fc-glycan	62:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	44	from	residues	30:37	arg1	arm					55:57	mannose α1-6 arm	42:57	mannose α1-6 arm of Fc-glycan	42:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	2	45	theme	complement-dependent	637:656	arg1	CDC					672:674	CDC	672:674	CDC	672:674	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	45	theme	complement-dependent	637:656	arg1	cytotoxicity					658:669	complement-dependent cytotoxicity	637:669	complement-dependent cytotoxicity (CDC)	637:675	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	5	46	theme	G2F	1180:1182	arg1	mAb					1184:1186	G2F mAb	1180:1186	G2F mAb	1180:1186	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	9	47	theme	growth	2267:2272	arg1	receptor					2281:2288	human epidermal growth factor receptor 2	2251:2290	human epidermal growth factor receptor 2	2251:2290	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	47	theme	growth	2267:2272	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	48	theme	core-fucosylated	256:271	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	48	theme	core-fucosylated	256:271	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	49	from	residue	1353:1359	arg1	arm					1377:1379	the Man α1-6 arm	1364:1379	the Man α1-6 arm	1364:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	50	theme	proximal	1493:1500	arg1	region					1502:1507	their hinge proximal region	1481:1507	their hinge proximal region in the CH2 domain	1481:1525	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	0	51	theme	terminal	11:18	arg1	residues					30:37	terminal galactose residues	11:37	terminal galactose residues in mannose α1-6 arm of Fc-glycan	11:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	1	52	theme	asialo-biantennary	277:294	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	52	theme	asialo-biantennary	277:294	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	9	53	theme	epidermal	2257:2265	arg1	receptor					2281:2288	human epidermal growth factor receptor 2	2251:2290	human epidermal growth factor receptor 2	2251:2290	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	53	theme	epidermal	2257:2265	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	3	54	theme	homogeneous	848:858	arg1	G2F					860:862	homogeneous G2F	848:862	homogeneous G2F	848:862	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	SGP					2586:2588	SGP	2586:2588	SGP	2586:2588	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	GST					2213:2215	GST	2213:2215	GST	2213:2215	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	HDX					2293:2295	HDX	2293:2295	HDX	2293:2295	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	HER2					2245:2248	HER2	2245:2248	HER2	2245:2248	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	55	dep	N-acetylglucosamine	2192:2210	arg1	chromatography					2365:2378	HILIC, hydrophilic interaction liquid chromatography	2327:2378	chromatography	2365:2378	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	56	theme	high-performance	2451:2466	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	56	theme	high-performance	2451:2466	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	57	theme	residues	30:37	arg1	Effects					0:6	Effects	0:6	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.	0:134	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	9	58	theme	factor	2274:2279	arg1	receptor					2281:2288	human epidermal growth factor receptor 2	2251:2290	human epidermal growth factor receptor 2	2251:2290	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	58	theme	factor	2274:2279	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	3	59	theme	biological	961:970	arg1	activities					972:981	their biological activities	955:981	their biological activities	955:981	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	9	60	theme	interaction	2346:2356	arg1	chromatography					2365:2378	HILIC, hydrophilic interaction liquid chromatography	2327:2378	chromatography	2365:2378	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	61	theme	ammonium	1844:1851	arg1	solution					1865:1872	ammonium bicarbonate solution	1844:1872	ammonium bicarbonate solution	1844:1872	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	62	theme	formic	2092:2097	arg1	acid					2099:2102	formic acid	2092:2102	formic acid	2092:2102	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	63	theme	mannose	42:48	arg1	Fc-glycan					62:70	Fc-glycan	62:70	Fc-glycan	62:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	63	theme	mannose	42:48	arg1	arm					55:57	mannose α1-6 arm	42:57	mannose α1-6 arm of Fc-glycan	42:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	9	64	theme	mass	2535:2538	arg1	antibody					2507:2514	monoclonal antibody	2496:2514	monoclonal antibody	2496:2514	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	64	theme	mass	2535:2538	arg1	spectrometry					2540:2551	mass spectrometry	2535:2551	mass spectrometry	2535:2551	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	6	65	theme	terminal	1340:1347	arg1	residue					1353:1359	the terminal Gal residue	1336:1359	the terminal Gal residue on the Man α1-6 arm	1336:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	66	theme	effector	1662:1669	arg1	functions					1671:1679	the effector functions	1658:1679	the effector functions of mAbs	1658:1687	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	9	67	dep	antibody	2507:2514	arg1	PBS					2554:2556	PBS	2554:2556	PBS	2554:2556	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	67	dep	antibody	2507:2514	arg1	MS					2531:2532	MS	2531:2532	MS	2531:2532	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	67	dep	antibody	2507:2514	arg1	Man					2517:2519	Man	2517:2519	Man	2517:2519	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	3	68	theme	G2F	885:887	arg1	mAb					889:891	G2F mAb	885:891	G2F mAb	885:891	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	9	69	theme	liquid	2468:2473	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	69	theme	liquid	2468:2473	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	70	theme	monoclonal	223:232	arg1	mAbs					246:249	mAbs	246:249	mAbs	246:249	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	70	theme	monoclonal	223:232	arg1	antibodies					234:243	therapeutic monoclonal antibodies	211:243	therapeutic monoclonal antibodies (mAbs)	211:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	71	theme	spectrometry	1248:1259	arg1	analysis					1261:1268	Hydrogen-deuterium exchange/mass spectrometry analysis	1215:1268	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs	1215:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	72	theme	α1-6	1614:1617	arg1	Fc-glycans					1626:1635	Fc-glycans	1626:1635	Fc-glycans	1626:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	72	theme	α1-6	1614:1617	arg1	arm					1619:1621	the Man α1-6 arm	1606:1621	the Man α1-6 arm of Fc-glycans	1606:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	6	73	theme	greater	1313:1319	arg1	involvement					1321:1331	the greater involvement	1309:1331	the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain	1309:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	9	74	theme	egg	2595:2597	arg1	sialylglycopeptides					2604:2622	hen egg yolk sialylglycopeptides	2591:2622	hen egg yolk sialylglycopeptides	2591:2622	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	75	theme	antibody-dependent	1900:1917	arg1	cytotoxicity					1933:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	antibody-dependent cell-mediated cytotoxicity	1900:1944	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	76	theme	terminal	411:418	arg1	galactosylation					420:434	terminal galactosylation	411:434	terminal galactosylation on the Man α1-3 arm	411:454	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	9	77	theme	Fcγ	2140:2142	arg1	receptors					2144:2152	FcγR, Fcγ receptors	2134:2152	FcγR, Fcγ receptors	2134:2152	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	78	theme	Fc	169:170	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	78	theme	Fc	169:170	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	79	theme	exchange/mass	1234:1246	arg1	analysis					1261:1268	Hydrogen-deuterium exchange/mass spectrometry analysis	1215:1268	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs	1215:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	2	80	theme	effector	560:567	arg1	cytotoxicity					658:669	complement-dependent cytotoxicity	637:669	complement-dependent cytotoxicity (CDC)	637:675	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	80	theme	effector	560:567	arg1	cytotoxicity					620:631	antibody-dependent cell-mediated cytotoxicity	587:631	antibody-dependent cell-mediated cytotoxicity	587:631	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	80	theme	effector	560:567	arg1	functions					569:577	effector functions	560:577	effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC)	560:675	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	7	81	theme	terminal	1582:1589	arg1	residue					1595:1601	the terminal Gal residue	1578:1601	the terminal Gal residue on the Man α1-6 arm of Fc-glycans	1578:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	2	82	theme	G1F	700:702	arg1	isomers					704:710	the G1F isomers	696:710	the G1F isomers (G1aF and G1bF)	696:726	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	82	theme	G1F	700:702	arg1	G1bF					722:725	G1bF	722:725	G1bF	722:725	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	82	theme	G1F	700:702	arg1	G1aF					713:716	G1aF	713:716	G1aF	713:716	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	6	83	theme	α1-6	1372:1375	arg1	arm					1377:1379	the Man α1-6 arm	1364:1379	the Man α1-6 arm	1364:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	3	84	theme	G1bF	904:907	arg1	mAb					909:911	G1bF mAb	904:911	G1bF mAb	904:911	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	9	85	theme	yolk	2599:2602	arg1	sialylglycopeptides					2604:2622	hen egg yolk sialylglycopeptides	2591:2622	hen egg yolk sialylglycopeptides	2591:2622	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	0	86	theme	monoclonal	113:122	arg1	antibodies					124:133	therapeutic monoclonal antibodies	101:133	therapeutic monoclonal antibodies	101:133	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	1	87	theme	Man	443:445	arg1	arm					452:454	the Man α1-3 arm	439:454	the Man α1-3 arm	439:454	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	8	88	theme	dynamic	1797:1803	arg1	structure					1805:1813	dynamic structure	1797:1813	dynamic structure	1797:1813	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	9	89	theme	HLB-SPE	2381:2387	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	89	theme	HLB-SPE	2381:2387	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	2	90	theme	Terminal	489:496	arg1	Gal					509:511	Gal	509:511	Gal	509:511	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	2	90	theme	Terminal	489:496	arg1	galactose					498:506	Terminal galactose	489:506	Terminal galactose (Gal) residues of Fc-glycans	489:535	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	6	91	theme	Man	1368:1370	arg1	arm					1377:1379	the Man α1-6 arm	1364:1379	the Man α1-6 arm	1364:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	6	92	theme	residue	1353:1359	arg1	involvement					1321:1331	the greater involvement	1309:1331	the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain	1309:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	1	93	theme	CH2	197:199	arg1	domain					201:206	the CH2 domain	193:206	the CH2 domain in therapeutic monoclonal antibodies (mAbs)	193:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	9	94	theme	hydrophilic-lipophilic	2390:2411	arg1	chromatography					2475:2488	high-performance liquid chromatography	2451:2488	high-performance liquid chromatography	2451:2488	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	94	theme	hydrophilic-lipophilic	2390:2411	arg1	extraction					2433:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	hydrophilic-lipophilic balance-solid-phase extraction	2390:2442	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	8	95	from	impact	1745:1750	arg1	functions					1783:1791	effector functions	1774:1791	effector functions	1774:1791	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	8	95	from	impact	1745:1750	arg1	structure					1805:1813	dynamic structure	1797:1813	dynamic structure	1797:1813	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	2	96	from	impact	686:691	arg1	functions					744:752	the effector functions	731:752	the effector functions	731:752	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	9	97	gly	sialylglycopeptides	2604:2622	arg2	sialylglycopeptides					2604:2622	hen egg yolk sialylglycopeptides	2591:2622	hen egg yolk sialylglycopeptides	2591:2622	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	8	98	theme	first	1720:1724	arg1	this					1708:1711	this	1708:1711	this	1708:1711	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	8	98	theme	first	1720:1724	arg1	report					1726:1731	the first report	1716:1731	the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs	1716:1821	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	1	99	from	domain	201:206	arg1	mAbs					246:249	mAbs	246:249	mAbs	246:249	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	99	from	domain	201:206	arg1	antibodies					234:243	therapeutic monoclonal antibodies	211:243	therapeutic monoclonal antibodies (mAbs)	211:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	7	100	from	region	1502:1507	arg1	domain					1520:1525	the CH2 domain	1512:1525	the CH2 domain	1512:1525	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	4	101	theme	CDC	1056:1058	arg1	activity					1060:1067	CDC activity	1056:1067	CDC activity	1056:1067	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	5	102	theme	G1aF	1132:1135	arg1	mAb					1137:1139	G1aF mAb	1132:1139	G1aF mAb	1132:1139	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	1	103	theme	α1-6	394:397	arg1	arm					399:401	the Man α1-6 arm	386:401	the Man α1-6 arm	386:401	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	104	theme	CH2	1416:1418	arg1	domain					1420:1425	the CH2 domain	1412:1425	the CH2 domain	1412:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	105	theme	domain	1568:1573	arg1	stabilization					1543:1555	the structural stabilization	1528:1555	the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans	1528:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	105	theme	domain	1568:1573	arg1	important					1644:1652	important	1644:1652	important	1644:1652	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	8	106	dep	functions	1783:1791	arg1	the					1770:1772	the	1770:1772	the	1770:1772	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	4	107	theme	higher	1015:1020	arg1	C1q-					1022:1025	higher C1q-	1015:1025	higher C1q-	1015:1025	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	9	108	theme	complement-dependent	1982:2001	arg1	cytotoxicity					2003:2014	complement-dependent cytotoxicity	1982:2014	complement-dependent cytotoxicity	1982:2014	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	6	109	theme	Hydrogen-deuterium	1215:1232	arg1	analysis					1261:1268	Hydrogen-deuterium exchange/mass spectrometry analysis	1215:1268	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs	1215:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	1	110	theme	Typical	136:142	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	110	theme	Typical	136:142	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	111	dep	G1bF	405:408	arg1	galactosylation					420:434	terminal galactosylation	411:434	terminal galactosylation on the Man α1-3 arm	411:454	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	8	112	theme	G1F	1755:1757	arg1	isomers					1759:1765	G1F isomers	1755:1765	G1F isomers	1755:1765	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	6	113	theme	structural	1388:1397	arg1	stability					1399:1407	the structural stability	1384:1407	the structural stability of the CH2 domain	1384:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	1	114	theme	fragment	159:166	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	114	theme	fragment	159:166	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	2	115	theme	cell-mediated	606:618	arg1	cytotoxicity					620:631	antibody-dependent cell-mediated cytotoxicity	587:631	antibody-dependent cell-mediated cytotoxicity	587:631	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	0	116	theme	effector	79:86	arg1	functions					88:96	the effector functions	75:96	the effector functions of therapeutic monoclonal antibodies	75:133	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	3	117	theme	G0F	917:919	arg1	mAb					921:923	G0F mAb	917:923	G0F mAb	917:923	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	6	118	from	arm	1377:1379	arg1	involvement					1321:1331	the greater involvement	1309:1331	the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain	1309:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	5	119	theme	same	1166:1169	arg1	level					1171:1175	the same level	1162:1175	the same level	1162:1175	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	9	120	theme	quality	2031:2037	arg1	attribute					2039:2047	critical quality attribute	2022:2047	critical quality attribute	2022:2047	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	8	121	theme	effector	1774:1781	arg1	functions					1783:1791	effector functions	1774:1791	effector functions	1774:1791	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	7	122	theme	CH2	1516:1518	arg1	domain					1520:1525	the CH2 domain	1512:1525	the CH2 domain	1512:1525	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	9	123	theme	monoclonal	2496:2505	arg1	antibody					2507:2514	monoclonal antibody	2496:2514	monoclonal antibody	2496:2514	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	123	theme	monoclonal	2496:2505	arg1	saline					2578:2583	phosphate-buffered saline	2559:2583	phosphate-buffered saline	2559:2583	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	123	theme	monoclonal	2496:2505	arg1	spectrometry					2540:2551	mass spectrometry	2535:2551	mass spectrometry	2535:2551	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	123	theme	monoclonal	2496:2505	arg1	mannose					2522:2528	mannose	2522:2528	mannose	2522:2528	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	123	theme	monoclonal	2496:2505	arg1	mAb					2491:2493	mAb	2491:2493	mAb	2491:2493	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	124	dep	extraction	2433:2442	arg1	antibody					2507:2514	monoclonal antibody	2496:2514	monoclonal antibody	2496:2514	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	124	dep	extraction	2433:2442	arg1	HPLC					2445:2448	HPLC	2445:2448	HPLC	2445:2448	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	124	dep	extraction	2433:2442	arg1	mAb					2491:2493	mAb	2491:2493	mAb	2491:2493	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	6	125	theme	mAbs	1295:1298	arg1	structures					1281:1290	dynamic structures	1273:1290	dynamic structures of mAbs	1273:1298	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	3	126	theme	anti-CD20	825:833	arg1	mAbs					835:838	glycoengineered anti-CD20 mAbs	809:838	glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively)	809:938	Here, we prepared four types of glycoengineered anti-CD20 mAbs bearing homogeneous G2F, G1aF, G1bF, or G0F (G2F mAb, G1aF mAb, G1bF mAb, or G0F mAb, respectively), and evaluated their biological activities.					
30990348	4	127	theme	FcγR-activation	1074:1088	arg1	property					1090:1097	FcγR-activation property	1074:1097	FcγR-activation property	1074:1097	Interestingly, G1aF mAb showed higher C1q- and FcγR-binding activities, CDC activity, and FcγR-activation property than G1bF mAb.					
30990348	5	128	theme	mAb	1150:1152	arg1	activities					1118:1127	The activities	1114:1127	The activities of G1aF mAb and G1bF mAb	1114:1152	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	0	129	theme	galactose	20:28	arg1	residues					30:37	terminal galactose residues	11:37	terminal galactose residues in mannose α1-6 arm of Fc-glycan	11:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	9	130	theme	human	2251:2255	arg1	receptor					2281:2288	human epidermal growth factor receptor 2	2251:2290	human epidermal growth factor receptor 2	2251:2290	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	130	theme	human	2251:2255	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	1	131	dep	G2F	324:326	arg1	e.g.					318:321	e.g.	318:321	e.g.	318:321	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	8	132	theme	mAbs	1818:1821	arg1	functions					1783:1791	effector functions	1774:1791	effector functions	1774:1791	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	8	132	theme	mAbs	1818:1821	arg1	structure					1805:1813	dynamic structure	1797:1813	dynamic structure	1797:1813	To our knowledge, this is the first report showing the impact of G1F isomers on the effector functions and dynamic structure of mAbs.					
30990348	1	133	theme	complex-type	296:307	arg1	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	133	theme	complex-type	296:307	arg1	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	7	134	theme	mAbs	1684:1687	arg1	functions					1671:1679	the effector functions	1658:1679	the effector functions of mAbs	1658:1687	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	135	theme	hinge	1487:1491	arg1	region					1502:1507	their hinge proximal region	1481:1507	their hinge proximal region in the CH2 domain	1481:1525	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	1	136	dep	G1aF	352:355	arg1	galactosylation					367:381	terminal galactosylation	358:381	terminal galactosylation on the Man α1-6 arm	358:401	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	137	attach	attached	181:188	arg1	domain					201:206	the CH2 domain	193:206	the CH2 domain in therapeutic monoclonal antibodies (mAbs)	193:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	137	attach	attached	181:188	arg2	glycans					309:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	core-fucosylated and asialo-biantennary complex-type glycans	256:315	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	137	attach	attached	181:188	arg2	glycans					173:179	Typical crystallizable fragment (Fc) glycans	136:179	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs)	136:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	138	theme	α1-3	447:450	arg1	arm					452:454	the Man α1-3 arm	439:454	the Man α1-3 arm	439:454	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	0	139	theme	α1-6	50:53	arg1	Fc-glycan					62:70	Fc-glycan	62:70	Fc-glycan	62:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	0	139	theme	α1-6	50:53	arg1	arm					55:57	mannose α1-6 arm	42:57	mannose α1-6 arm of Fc-glycan	42:70	Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.					
30990348	9	140	theme	hydrogen-deuterium	2298:2315	arg1	exchange					2317:2324	hydrogen-deuterium exchange	2298:2324	hydrogen-deuterium exchange	2298:2324	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	140	theme	hydrogen-deuterium	2298:2315	arg1	N-acetylglucosamine					2192:2210	N-acetylglucosamine	2192:2210	N-acetylglucosamine	2192:2210	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	9	141	theme	bicarbonate	1853:1863	arg1	solution					1865:1872	ammonium bicarbonate solution	1844:1872	ammonium bicarbonate solution	1844:1872	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
30990348	6	142	theme	Gal	1349:1351	arg1	residue					1353:1359	the terminal Gal residue	1336:1359	the terminal Gal residue on the Man α1-6 arm	1336:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	1	143	from	galactosylation	420:434	arg1	arm					452:454	the Man α1-3 arm	439:454	the Man α1-3 arm	439:454	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	2	144	theme	effector	735:742	arg1	functions					744:752	the effector functions	731:752	the effector functions	731:752	Terminal galactose (Gal) residues of Fc-glycans are known to influence effector functions such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity (CDC), but the impact of the G1F isomers (G1aF and G1bF) on the effector functions has not been reported.					
30990348	1	145	theme	therapeutic	211:221	arg1	mAbs					246:249	mAbs	246:249	mAbs	246:249	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	1	145	theme	therapeutic	211:221	arg1	antibodies					234:243	therapeutic monoclonal antibodies	211:243	therapeutic monoclonal antibodies (mAbs)	211:250	Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans, e.g., G2F (full galactosylation), G1aF (terminal galactosylation on the Man α1-6 arm), G1bF (terminal galactosylation on the Man α1-3 arm), and G0F (non-galactosylation).					
30990348	6	146	from	involvement	1321:1331	arg1	arm					1377:1379	the Man α1-6 arm	1364:1379	the Man α1-6 arm	1364:1379	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	6	146	from	involvement	1321:1331	arg1	stability					1399:1407	the structural stability	1384:1407	the structural stability of the CH2 domain	1384:1425	Hydrogen-deuterium exchange/mass spectrometry analysis of dynamic structures of mAbs revealed the greater involvement of the terminal Gal residue on the Man α1-6 arm in the structural stability of the CH2 domain.					
30990348	7	147	theme	Fc-glycans	1626:1635	arg1	Fc-glycans					1626:1635	Fc-glycans	1626:1635	Fc-glycans	1626:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	147	theme	Fc-glycans	1626:1635	arg1	arm					1619:1621	the Man α1-6 arm	1606:1621	the Man α1-6 arm of Fc-glycans	1606:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	148	from	residue	1595:1601	arg1	Fc-glycans					1626:1635	Fc-glycans	1626:1635	Fc-glycans	1626:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	7	148	from	residue	1595:1601	arg1	arm					1619:1621	the Man α1-6 arm	1606:1621	the Man α1-6 arm of Fc-glycans	1606:1635	Considering that mAbs interact with FcγR and C1q via their hinge proximal region in the CH2 domain, the structural stabilization of the CH2 domain by the terminal Gal residue on the Man α1-6 arm of Fc-glycans may be important for the effector functions of mAbs.					
30990348	5	149	theme	G0F	1192:1194	arg1	mAb					1196:1198	G0F mAb	1192:1198	G0F mAb	1192:1198	The activities of G1aF mAb and G1bF mAb were at the same level as G2F mAb and G0F mAb, respectively.					
30990348	9	150	theme	crystallizable	2109:2122	arg1	fragment					2124:2131	crystallizable fragment	2109:2131	crystallizable fragment	2109:2131	Abbreviations: ABC, ammonium bicarbonate solution; ACN, acetonitrile; ADCC, antibody-dependent cell-mediated cytotoxicity; C1q, complement component 1q; CDC, complement-dependent cytotoxicity; CQA, critical quality attribute; Endo, endo-β-N-acetylglucosaminidase; FA, formic acid; Fc, crystallizable fragment; FcγR, Fcγ receptors; Fuc, fucose; Gal, galactose; GlcNAc, N-acetylglucosamine; GST, glutathione S-transferase; HER2, human epidermal growth factor receptor 2; HDX, hydrogen-deuterium exchange; HILIC, hydrophilic interaction liquid chromatography; HLB-SPE, hydrophilic-lipophilic balance-solid-phase extraction; HPLC, high-performance liquid chromatography; mAb, monoclonal antibody; Man, mannose; MS, mass spectrometry; PBS, phosphate-buffered saline; SGP, hen egg yolk sialylglycopeptides.					
31841688	4	0	theme	C-terminal	946:955	arg1	Lys					957:959	C-terminal Lys	946:959	C-terminal Lys	946:959	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	1	theme	soluble	1002:1008	arg1	aggregates					1010:1019	soluble aggregates	1002:1019	soluble aggregates	1002:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	0	2	theme	microheterogeneity	73:90	arg1	impact					56:61	The impact	52:61	The impact of glycan microheterogeneity on Fc function	52:105	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	1	3	theme	patient	161:167	arg1	access					169:174	patient access	161:174	patient access to expensive, but highly effective biologic products	161:227	Biosimilars are poised to reduce prices and increase patient access to expensive, but highly effective biologic products.					
31841688	2	4	from	information	309:319	arg1	products					335:342	biosimilar products	324:342	biosimilar products from outside of the US/EU in the public domain	324:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	5	5	theme	afucosylated	1086:1097	arg1	glycans					1099:1105	afucosylated glycans	1086:1105	afucosylated glycans	1086:1105	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	6	6	theme	comprehensive	1222:1234	arg1	comparison					1236:1245	a comprehensive comparison	1220:1245	a comprehensive comparison of Rituxan® and Acellbia®	1220:1271	Taken together, our work provides a comprehensive comparison of Rituxan® and Acellbia®.					
31841688	2	7	theme	similarity	282:291	arg1	degree					272:277	the degree	268:277	the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain	268:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	5	8	theme	increased	1161:1169	arg1	activity					1176:1183	increased ADCC activity	1161:1183	increased ADCC activity	1161:1183	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	0	9	theme	Fc	95:96	arg1	function					98:105	Fc function	95:105	Fc function	95:105	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	0	10	from	impact	56:61	arg1	function					98:105	Fc function	95:105	Fc function	95:105	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	4	11	theme	MS	739:740	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	5	12	theme	ADCC	1171:1174	arg1	activity					1176:1183	increased ADCC activity	1161:1183	increased ADCC activity	1161:1183	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	4	13	theme	glycoform	913:921	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	2	14	from	products	335:342	arg1	scarcity					297:304	scarcity	297:304	scarcity of information on biosimilar products from outside of the US/EU in the public domain	297:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	2	14	from	products	335:342	arg1	similarity					282:291	similarity	282:291	similarity	282:291	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	4	15	theme	products	639:646	arg1	biosimilarity					612:624	biosimilarity	612:624	biosimilarity of these two products	612:646	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	5	16	gly	afucosylated	1086:1097	arg1	glycans					1099:1105	afucosylated glycans	1086:1105	afucosylated glycans	1086:1105	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	2	17	theme	public	377:382	arg1	domain					384:389	the public domain	373:389	the public domain	373:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	4	18	theme	charge	982:987	arg1	variants					989:996	charge variants	982:996	charge variants	982:996	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	19	theme	levels	936:941	arg1	terms					904:908	terms	904:908	terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	904:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	2	20	theme	information	309:319	arg1	scarcity					297:304	scarcity	297:304	scarcity of information on biosimilar products from outside of the US/EU in the public domain	297:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	2	20	theme	information	309:319	arg1	similarity					282:291	similarity	282:291	similarity	282:291	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	6	21	theme	Acellbia®	1263:1271	arg1	comparison					1236:1245	a comprehensive comparison	1220:1245	a comprehensive comparison of Rituxan® and Acellbia®	1220:1271	Taken together, our work provides a comprehensive comparison of Rituxan® and Acellbia®.					
31841688	1	22	theme	expensive	179:187	arg1	products					220:227	expensive, but highly effective biologic products	179:227	expensive, but highly effective biologic products	179:227	Biosimilars are poised to reduce prices and increase patient access to expensive, but highly effective biologic products.					
31841688	0	23	theme	Multifaceted	0:11	arg1	assessment					13:22	Multifaceted assessment	0:22	Multifaceted assessment of rituximab biosimilarity	0:49	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	2	24	from	US/EU	364:368	arg1	domain					384:389	the public domain	373:389	the public domain	373:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	3	25	theme	rituximab	541:549	arg1	entity					416:421	an independent entity	401:421	an independent entity	401:421	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	25	theme	rituximab	541:549	arg1	Acellbia®					563:571	Acellbia®	563:571	Acellbia®	563:571	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	25	theme	rituximab	541:549	arg1	biosimilar					551:560	a Russian rituximab biosimilar	531:560	a Russian rituximab biosimilar	531:560	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	25	theme	rituximab	541:549	arg1	analysis					451:458	a comparative analysis	437:458	a comparative analysis between the innovator	437:480	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	25	theme	rituximab	541:549	arg1	Rituxan®					483:490	Rituxan®	483:490	Rituxan® (manufactured by Genentech/Roche)	483:524	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	26	theme	independent	404:414	arg1	entity					416:421	an independent entity	401:421	an independent entity	401:421	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	26	theme	independent	404:414	arg1	Rituxan®					483:490	Rituxan®	483:490	Rituxan® (manufactured by Genentech/Roche)	483:524	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	26	theme	independent	404:414	arg1	analysis					451:458	a comparative analysis	437:458	a comparative analysis between the innovator	437:480	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	26	theme	independent	404:414	arg1	biosimilar					551:560	a Russian rituximab biosimilar	531:560	a Russian rituximab biosimilar	531:560	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	0	27	theme	rituximab	27:35	arg1	biosimilarity					37:49	rituximab biosimilarity	27:49	rituximab biosimilarity	27:49	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	5	28	theme	stronger	1123:1130	arg1	affinity					1148:1155	a stronger FcγIIIa binding affinity	1121:1155	a stronger FcγIIIa binding affinity	1121:1155	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	2	29	theme	scarcity	297:304	arg1	degree					272:277	the degree	268:277	the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain	268:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	4	30	theme	techniques	710:719	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	30	theme	techniques	710:719	arg1	HX-MS					751:755	HX-MS	751:755	HX-MS	751:755	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	30	theme	techniques	710:719	arg1	variety					653:659	a variety	651:659	a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS.	651:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	30	theme	techniques	710:719	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	30	theme	techniques	710:719	arg1	techniques					710:719	state-of-the-art analytical mass spectrometry techniques	664:719	state-of-the-art analytical mass spectrometry techniques	664:719	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	30	theme	techniques	710:719	arg1	IM-MS					758:762	IM-MS	758:762	IM-MS	758:762	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	5	31	theme	FcγIIIa	1132:1138	arg1	affinity					1148:1155	a stronger FcγIIIa binding affinity	1121:1155	a stronger FcγIIIa binding affinity	1121:1155	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	3	32	theme	comparative	439:449	arg1	entity					416:421	an independent entity	401:421	an independent entity	401:421	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	32	theme	comparative	439:449	arg1	Rituxan®					483:490	Rituxan®	483:490	Rituxan® (manufactured by Genentech/Roche)	483:524	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	32	theme	comparative	439:449	arg1	analysis					451:458	a comparative analysis	437:458	a comparative analysis between the innovator	437:480	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	32	theme	comparative	439:449	arg1	biosimilar					551:560	a Russian rituximab biosimilar	531:560	a Russian rituximab biosimilar	531:560	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	5	33	theme	binding	1140:1146	arg1	affinity					1148:1155	a stronger FcγIIIa binding affinity	1121:1155	a stronger FcγIIIa binding affinity	1121:1155	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	0	34	dep	impact	56:61	arg1	assessment					13:22	Multifaceted assessment	0:22	Multifaceted assessment of rituximab biosimilarity	0:49	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	1	35	theme	effective	201:209	arg1	products					220:227	expensive, but highly effective biologic products	179:227	expensive, but highly effective biologic products	179:227	Biosimilars are poised to reduce prices and increase patient access to expensive, but highly effective biologic products.					
31841688	0	36	theme	biosimilarity	37:49	arg1	assessment					13:22	Multifaceted assessment	0:22	Multifaceted assessment of rituximab biosimilarity	0:49	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	5	37	theme	glycans	1099:1105	arg1	level					1077:1081	a higher level	1068:1081	a higher level of afucosylated glycans	1068:1105	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	4	38	theme	Lys	957:959	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	39	theme	intact	769:774	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	1	40	theme	biologic	211:218	arg1	products					220:227	expensive, but highly effective biologic products	179:227	expensive, but highly effective biologic products	179:227	Biosimilars are poised to reduce prices and increase patient access to expensive, but highly effective biologic products.					
31841688	4	41	theme	pyroGlu	973:979	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	42	theme	order	849:853	arg1	structure					855:863	higher order structure	842:863	higher order structure	842:863	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	43	theme	analytical	681:690	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	43	theme	analytical	681:690	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	43	theme	analytical	681:690	arg1	techniques					710:719	state-of-the-art analytical mass spectrometry techniques	664:719	state-of-the-art analytical mass spectrometry techniques	664:719	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	43	theme	analytical	681:690	arg1	IM-MS					758:762	IM-MS	758:762	IM-MS	758:762	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	43	theme	analytical	681:690	arg1	HX-MS					751:755	HX-MS	751:755	HX-MS	751:755	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	44	theme	N-terminal	962:971	arg1	pyroGlu					973:979	N-terminal pyroGlu	962:979	N-terminal pyroGlu	962:979	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	45	theme	higher	842:847	arg1	structure					855:863	higher order structure	842:863	higher order structure	842:863	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	46	theme	state-of-the-art	664:679	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	46	theme	state-of-the-art	664:679	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	46	theme	state-of-the-art	664:679	arg1	techniques					710:719	state-of-the-art analytical mass spectrometry techniques	664:719	state-of-the-art analytical mass spectrometry techniques	664:719	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	46	theme	state-of-the-art	664:679	arg1	IM-MS					758:762	IM-MS	758:762	IM-MS	758:762	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	46	theme	state-of-the-art	664:679	arg1	HX-MS					751:755	HX-MS	751:755	HX-MS	751:755	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	3	47	theme	Russian	533:539	arg1	entity					416:421	an independent entity	401:421	an independent entity	401:421	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	47	theme	Russian	533:539	arg1	Acellbia®					563:571	Acellbia®	563:571	Acellbia®	563:571	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	47	theme	Russian	533:539	arg1	biosimilar					551:560	a Russian rituximab biosimilar	531:560	a Russian rituximab biosimilar	531:560	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	47	theme	Russian	533:539	arg1	analysis					451:458	a comparative analysis	437:458	a comparative analysis between the innovator	437:480	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	3	47	theme	Russian	533:539	arg1	Rituxan®					483:490	Rituxan®	483:490	Rituxan® (manufactured by Genentech/Roche)	483:524	Thus, as an independent entity, we performed a comparative analysis between the innovator, Rituxan® (manufactured by Genentech/Roche), and a Russian rituximab biosimilar, Acellbia® (manufactured by Biocad).					
31841688	4	48	dep	evaluated	602:610	arg1	Both					780:783	Both	780:783	Both	780:783	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	2	49	theme	biosimilar	324:333	arg1	products					335:342	biosimilar products	324:342	biosimilar products from outside of the US/EU in the public domain	324:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	4	50	theme	tandem	732:737	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	6	51	theme	Rituxan®	1250:1257	arg1	comparison					1236:1245	a comprehensive comparison	1220:1245	a comprehensive comparison of Rituxan® and Acellbia®	1220:1271	Taken together, our work provides a comprehensive comparison of Rituxan® and Acellbia®.					
31841688	4	52	theme	mass	692:695	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	52	theme	mass	692:695	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	52	theme	mass	692:695	arg1	techniques					710:719	state-of-the-art analytical mass spectrometry techniques	664:719	state-of-the-art analytical mass spectrometry techniques	664:719	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	52	theme	mass	692:695	arg1	IM-MS					758:762	IM-MS	758:762	IM-MS	758:762	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	52	theme	mass	692:695	arg1	HX-MS					751:755	HX-MS	751:755	HX-MS	751:755	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	2	53	from	similarity	282:291	arg1	products					335:342	biosimilar products	324:342	biosimilar products from outside of the US/EU in the public domain	324:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	4	54	theme	spectrometry	697:708	arg1	MS.					776:778	intact MS.	769:778	intact MS.	769:778	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	54	theme	spectrometry	697:708	arg1	mapping					742:748	tandem MS mapping	732:748	tandem MS mapping	732:748	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	54	theme	spectrometry	697:708	arg1	techniques					710:719	state-of-the-art analytical mass spectrometry techniques	664:719	state-of-the-art analytical mass spectrometry techniques	664:719	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	54	theme	spectrometry	697:708	arg1	IM-MS					758:762	IM-MS	758:762	IM-MS	758:762	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	54	theme	spectrometry	697:708	arg1	HX-MS					751:755	HX-MS	751:755	HX-MS	751:755	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	0	55	theme	glycan	66:71	arg1	microheterogeneity					73:90	glycan microheterogeneity	66:90	glycan microheterogeneity	66:90	Multifaceted assessment of rituximab biosimilarity: The impact of glycan microheterogeneity on Fc function.					
31841688	4	56	theme	aggregates	1010:1019	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	4	57	theme	distribution	923:934	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	5	58	theme	higher	1070:1075	arg1	level					1077:1081	a higher level	1068:1081	a higher level of afucosylated glycans	1068:1105	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	4	59	theme	variants	989:996	arg1	levels					936:941	glycoform distribution levels	913:941	glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates	913:1019	We evaluated biosimilarity of these two products by a variety of state-of-the-art analytical mass spectrometry techniques, including tandem MS mapping, HX-MS, IM-MS, and intact MS. Both were found to be generally similar regarding primary and higher order structure, though differences were identified in terms of glycoform distribution levels of C-terminal Lys, N-terminal pyroGlu, charge variants and soluble aggregates.					
31841688	2	60	from	scarcity	297:304	arg1	products					335:342	biosimilar products	324:342	biosimilar products from outside of the US/EU in the public domain	324:389	However, questions still remain about the degree of similarity and scarcity of information on biosimilar products from outside of the US/EU in the public domain.					
31841688	5	61	contain	had	1064:1066	arg2	level					1077:1081	a higher level	1068:1081	a higher level of afucosylated glycans	1068:1105	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31841688	5	61	contain	had	1064:1066	arg1	biosimilar					1053:1062	the biosimilar	1049:1062	the biosimilar	1049:1062	Notably, we confirmed that the biosimilar had a higher level of afucosylated glycans, resulting in a stronger FcγIIIa binding affinity and increased ADCC activity.					
31715490	0	0	theme	high-mannose	81:92	arg1	profile					103:109	high-mannose N-glycan profile	81:109	high-mannose N-glycan profile	81:109	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	4	1	theme	early	661:665	arg1	development					667:677	early development	661:677	early development of nodules and roots	661:698	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	1	2	theme	B	146:146	arg1	deficiency					149:158	Boron (B) deficiency	139:158	Boron (B) deficiency	139:158	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	1	3	theme	meristems	239:247	arg1	development					172:182	the development	168:182	the development of Pisum sativum nodules and Arabidopsis thaliana root meristems	168:247	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	5	4	theme	increasing	739:748	arg1	evidence					750:757	increasing evidence	739:757	increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	739:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	5	5	from	evidence	750:757	arg1	physiology					781:790	animal physiology	774:790	animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	774:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	2	6	theme	tumor-like	339:348	arg1	structures					350:359	tumor-like structures	339:359	tumor-like structures	339:359	Both organs show an alteration of cell differentiation that result in the development of tumor-like structures.					
31715490	6	7	theme	cleavage	935:942	arg1	stages					944:949	early cleavage stages	929:949	early cleavage stages	929:949	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	5	8	theme	deficiency	839:848	arg1	impact					827:832	the impact	823:832	the impact of B deficiency on Danio rerio (zebrafish) development	823:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	3	9	theme	high	530:533	arg1	HMNG					559:562	HMNG	559:562	HMNG	559:562	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	3	9	theme	high	530:533	arg1	N-glycans					548:556	high mannose type N-glycans	530:556	high mannose type N-glycans (HMNG)	530:563	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	6	10	theme	early	929:933	arg1	stages					944:949	early cleavage stages	929:949	early cleavage stages	929:949	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	0	11	theme	N-glycan	94:101	arg1	profile					103:109	high-mannose N-glycan profile	81:109	high-mannose N-glycan profile	81:109	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	4	12	theme	concanavalin	587:598	arg1	A					600:600	concanavalin A	587:600	concanavalin A	587:600	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	2	13	theme	structures	350:359	arg1	development					324:334	the development	320:334	the development of tumor-like structures	320:359	Both organs show an alteration of cell differentiation that result in the development of tumor-like structures.					
31715490	5	14	from	physiology	781:790	arg1	evidence					750:757	increasing evidence	739:757	increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	739:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	3	15	theme	cell	440:443	arg1	wall					445:448	the cell wall	436:448	the cell wall	436:448	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	8	16	theme	B-deficient	1208:1218	arg1	larvae					1230:1235	B-deficient zebrafish larvae	1208:1235	B-deficient zebrafish larvae prior to organogenesis	1208:1258	Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis.					
31715490	6	17	theme	undifferentiated	985:1000	arg1	mass					1002:1005	an abnormal undifferentiated mass	973:1005	an abnormal undifferentiated mass of cells	973:1014	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	3	18	from	B	376:376	arg1	plants					381:386	plants	381:386	plants	381:386	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	7	19	theme	aberrant	1083:1090	arg1	organogenesis					1092:1104	aberrant organogenesis	1083:1104	aberrant organogenesis	1083:1104	Additionally, when B was removed at post-hatching, larvae undergo aberrant organogenesis.					
31715490	8	20	theme	pattern	1188:1194	arg1	alteration					1154:1163	alteration	1154:1163	alteration of the N-glycosylation pattern	1154:1194	Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis.					
31715490	0	21	theme	plant	8:12	arg1	organogenesis					14:26	Altered plant organogenesis	0:26	Altered plant organogenesis under boron deficiency	0:49	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	5	22	theme	Danio	853:857	arg1	zebrafish					866:874	zebrafish	866:874	zebrafish	866:874	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	5	22	theme	Danio	853:857	arg1	rerio					859:863	Danio rerio	853:863	Danio rerio (zebrafish) development	853:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	3	23	theme	mannose	535:541	arg1	HMNG					559:562	HMNG	559:562	HMNG	559:562	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	3	23	theme	mannose	535:541	arg1	N-glycans					548:556	high mannose type N-glycans	530:556	high mannose type N-glycans (HMNG)	530:563	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	6	24	theme	cells	1010:1014	arg1	mass					1002:1005	an abnormal undifferentiated mass	973:1005	an abnormal undifferentiated mass of cells	973:1014	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	0	25	theme	Altered	0:6	arg1	organogenesis					14:26	Altered plant organogenesis	0:26	Altered plant organogenesis under boron deficiency	0:49	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	9	26	theme	common	1294:1299	arg1	important					1381:1389	important	1381:1389	important	1381:1389	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	9	26	theme	common	1294:1299	arg1	function					1301:1308	a common function	1292:1308	a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development	1292:1455	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	9	27	from	function	1301:1308	arg1	plants					1318:1323	plants	1318:1323	plants	1318:1323	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	9	27	from	function	1301:1308	arg1	animals					1329:1335	animals	1329:1335	animals	1329:1335	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	3	28	theme	type	543:546	arg1	HMNG					559:562	HMNG	559:562	HMNG	559:562	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	3	28	theme	type	543:546	arg1	N-glycans					548:556	high mannose type N-glycans	530:556	high mannose type N-glycans (HMNG)	530:563	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	4	29	from	alterations	611:621	arg1	pattern					646:652	the N-glycosylation pattern	626:652	the N-glycosylation pattern	626:652	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	4	30	theme	N-glycosylation	630:644	arg1	pattern					646:652	the N-glycosylation pattern	626:652	the N-glycosylation pattern	626:652	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	5	31	theme	animal	774:779	arg1	physiology					781:790	animal physiology	774:790	animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	774:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	9	32	theme	cell	1395:1398	arg1	signaling					1400:1408	cell signaling and cell fate determination	1395:1436	signaling	1400:1408	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	2	33	theme	differentiation	289:303	arg1	alteration					270:279	an alteration	267:279	an alteration of cell differentiation that result in the development of tumor-like structures	267:359	Both organs show an alteration of cell differentiation that result in the development of tumor-like structures.					
31715490	9	34	theme	fate	1419:1422	arg1	determination					1424:1436	cell signaling and cell fate determination	1395:1436	determination	1424:1436	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	4	35	theme	B	706:706	arg1	deprivation					708:718	B deprivation	706:718	B deprivation	706:718	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	2	36	theme	cell	284:287	arg1	differentiation					289:303	cell differentiation	284:303	cell differentiation	284:303	Both organs show an alteration of cell differentiation that result in the development of tumor-like structures.					
31715490	1	37	theme	Pisum	187:191	arg1	nodules					201:207	Pisum sativum nodules	187:207	Pisum sativum nodules	187:207	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	3	38	theme	membrane-associated	464:482	arg1	glycoconjugates					484:498	membrane-associated glycoconjugates	464:498	membrane-associated glycoconjugates	464:498	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	9	39	theme	B	1313:1313	arg1	important					1381:1389	important	1381:1389	important	1381:1389	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	9	39	theme	B	1313:1313	arg1	function					1301:1308	a common function	1292:1308	a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development	1292:1455	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	8	40	theme	zebrafish	1220:1228	arg1	larvae					1230:1235	B-deficient zebrafish larvae	1208:1235	B-deficient zebrafish larvae prior to organogenesis	1208:1258	Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis.					
31715490	9	41	theme	cell	1414:1417	arg1	determination					1424:1436	cell signaling and cell fate determination	1395:1436	determination	1424:1436	Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.					
31715490	6	42	theme	abnormal	976:983	arg1	mass					1002:1005	an abnormal undifferentiated mass	973:1005	an abnormal undifferentiated mass of cells	973:1014	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	0	43	theme	boron	34:38	arg1	deficiency					40:49	boron deficiency	34:49	boron deficiency	34:49	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	6	44	theme	B	895:895	arg1	deficiency					897:906	B deficiency	895:906	B deficiency	895:906	When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells.					
31715490	0	45	from	changes	70:76	arg1	profile					103:109	high-mannose N-glycan profile	81:109	high-mannose N-glycan profile	81:109	Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals.					
31715490	4	46	with	affinoblots	570:580	arg1	A					600:600	concanavalin A	587:600	concanavalin A	587:600	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	1	47	theme	nodules	201:207	arg1	development					172:182	the development	168:182	the development of Pisum sativum nodules and Arabidopsis thaliana root meristems	168:247	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	8	48	theme	N-glycosylation	1172:1186	arg1	pattern					1188:1194	the N-glycosylation pattern	1168:1194	the N-glycosylation pattern	1168:1194	Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis.					
31715490	5	49	theme	B	837:837	arg1	deficiency					839:848	B deficiency	837:848	B deficiency	837:848	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	1	50	dep	Arabidopsis	213:223	arg1	thaliana					225:232	thaliana	225:232	thaliana	225:232	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	5	51	from	role	766:769	arg1	physiology					781:790	animal physiology	774:790	animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	774:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	5	52	theme	rerio	859:863	arg1	development					877:887	Danio rerio (zebrafish) development	853:887	Danio rerio (zebrafish) development	853:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	5	53	theme	B	764:764	arg1	role					766:769	a B role	762:769	a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	762:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	4	54	theme	nodules	682:688	arg1	development					667:677	early development	661:677	early development of nodules and roots	661:698	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	3	55	theme	wall	445:448	arg1	components					422:431	components	422:431	components of the cell wall	422:448	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	8	56	theme	prior	1237:1241	arg1	larvae					1230:1235	B-deficient zebrafish larvae	1208:1235	B-deficient zebrafish larvae prior to organogenesis	1208:1258	Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis.					
31715490	5	57	from	impact	827:832	arg1	development					877:887	Danio rerio (zebrafish) development	853:887	Danio rerio (zebrafish) development	853:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	4	58	theme	roots	694:698	arg1	development					667:677	early development	661:677	early development of nodules and roots	661:698	The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation.					
31715490	1	59	theme	Arabidopsis	213:223	arg1	meristems					239:247	Arabidopsis thaliana root meristems	213:247	Arabidopsis thaliana root meristems	213:247	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	3	60	from	changes	519:525	arg1	HMNG					559:562	HMNG	559:562	HMNG	559:562	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	3	60	from	changes	519:525	arg1	N-glycans					548:556	high mannose type N-glycans	530:556	high mannose type N-glycans (HMNG)	530:563	The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG).					
31715490	1	61	theme	Boron	139:143	arg1	deficiency					149:158	Boron (B) deficiency	139:158	Boron (B) deficiency	139:158	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	1	62	dep	Pisum	187:191	arg1	sativum					193:199	sativum	193:199	sativum	193:199	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
31715490	5	63	theme	role	766:769	arg1	evidence					750:757	increasing evidence	739:757	increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development	739:887	Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development.					
31715490	1	64	theme	root	234:237	arg1	meristems					239:247	Arabidopsis thaliana root meristems	213:247	Arabidopsis thaliana root meristems	213:247	Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems.					
30540945	0	0	theme	Disassembly	83:93	arg1	Mechanisms					63:72	Mechanisms	63:72	Mechanisms of Virus Disassembly and Antibody Neutralization	63:121	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	8	1	theme	relevant	1166:1173	arg1	epitopes					1157:1164	protective epitopes	1146:1164	protective epitopes relevant to vaccine design	1146:1191	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	7	2	dep	showed	875:880	arg1	following					979:987	following	979:987	showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release	875:1002	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	6	3	theme	nucleocapsid	709:720	arg1	release					727:733	nucleocapsid core release	709:733	nucleocapsid core release	709:733	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	7	4	theme	viral	884:888	arg1	site					909:912	a viral RNA genome binding site	882:912	a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation	882:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	0	5	theme	Virus	77:81	arg1	Disassembly					83:93	Virus Disassembly	77:93	Virus Disassembly	77:93	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	4	6	theme	factor	508:513	arg1	sulfate					523:529	the cellular attachment factor heparan sulfate	484:529	the cellular attachment factor heparan sulfate	484:529	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	8	7	theme	Fab-EEEV	1016:1023	arg1	complexes					1025:1033	five Fab-EEEV complexes	1011:1033	five Fab-EEEV complexes derived from neutralizing antibodies	1011:1070	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	6	8	theme	capsid	805:810	arg1	dissociation					812:823	capsid dissociation	805:823	capsid dissociation from envelope proteins	805:846	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	0	9	theme	Antibody	99:106	arg1	Neutralization					108:121	Antibody Neutralization	99:121	Antibody Neutralization	99:121	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	4	10	theme	attachment	497:506	arg1	factor					508:513	cellular attachment factor	488:513	the cellular attachment factor heparan sulfate	484:529	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	7	11	theme	viral	954:958	arg1	translation					967:977	viral genome translation	954:977	viral genome translation	954:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	4	12	theme	binding	467:473	arg1	site					475:478	a binding site	465:478	a binding site for the cellular attachment factor heparan sulfate	465:529	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	7	13	theme	binding	937:943	arg1	site					945:948	a ribosome binding site	926:948	a ribosome binding site for viral genome translation	926:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	2	14	theme	virus	299:303	arg1	structure					258:266	a 4.4-Å cryoelectron microscopy (cryo-EM) structure	216:266	a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans	216:366	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	7	15	theme	EEEV	853:856	arg1	structure					865:873	The EEEV capsid structure	849:873	The EEEV capsid structure	849:873	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	5	16	theme	lectin-expressing	610:626	arg1	cells					644:648	lectin-expressing myeloid lineage cells	610:648	lectin-expressing myeloid lineage cells	610:648	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	16	theme	lectin-expressing	610:626	arg1	sentinels					661:669	sentinels	661:669	sentinels of the immune system	661:690	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	6	17	theme	pH	790:791	arg1	changes					793:799	pH changes	790:799	pH changes	790:799	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	2	18	theme	encephalitis	286:297	arg1	virus					299:303	eastern equine encephalitis virus	271:303	eastern equine encephalitis virus (EEEV)	271:310	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	2	18	theme	encephalitis	286:297	arg1	alphavirus					316:325	an alphavirus	313:325	an alphavirus that causes fatal encephalitis in humans	313:366	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	2	18	theme	encephalitis	286:297	arg1	EEEV					306:309	EEEV	306:309	EEEV	306:309	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	7	19	theme	capsid	858:863	arg1	structure					865:873	The EEEV capsid structure	849:873	The EEEV capsid structure	849:873	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	0	20	theme	Neutralization	108:121	arg1	Mechanisms					63:72	Mechanisms	63:72	Mechanisms of Virus Disassembly and Antibody Neutralization	63:121	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	5	21	theme	myeloid	628:634	arg1	cells					644:648	lectin-expressing myeloid lineage cells	610:648	lectin-expressing myeloid lineage cells	610:648	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	21	theme	myeloid	628:634	arg1	sentinels					661:669	sentinels	661:669	sentinels of the immune system	661:690	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	22	theme	E2	558:559	arg1	glycan					561:566	a cryptic E2 glycan	548:566	a cryptic E2 glycan	548:566	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	2	23	theme	equine	279:284	arg1	encephalitis					286:297	eastern equine encephalitis	271:297	eastern equine encephalitis virus (EEEV)	271:310	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	5	24	theme	lineage	636:642	arg1	cells					644:648	lectin-expressing myeloid lineage cells	610:648	lectin-expressing myeloid lineage cells	610:648	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	24	theme	lineage	636:642	arg1	sentinels					661:669	sentinels	661:669	sentinels of the immune system	661:690	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	25	theme	glycan	561:566	arg1	presence					536:543	The presence	532:543	The presence of a cryptic E2 glycan	532:566	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	3	26	theme	host	422:425	arg1	cells					427:431	host cells	422:431	host cells	422:431	Our analysis provides insights into viral entry into host cells.					
30540945	0	27	theme	Cryo-EM	0:6	arg1	Structures					8:17	Cryo-EM Structures	0:17	Cryo-EM Structures of Eastern Equine Encephalitis Virus	0:54	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	7	28	theme	adjacent	914:921	arg1	site					909:912	a viral RNA genome binding site	882:912	a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation	882:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	0	29	theme	Virus	50:54	arg1	Structures					8:17	Cryo-EM Structures	0:17	Cryo-EM Structures of Eastern Equine Encephalitis Virus	0:54	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	0	30	theme	Eastern	22:28	arg1	Encephalitis					37:48	Eastern Equine Encephalitis	22:48	Eastern Equine Encephalitis Virus	22:54	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	4	31	theme	protein	447:453	arg1	E2					455:456	The envelope protein E2	434:456	The envelope protein E2	434:456	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	0	32	theme	Encephalitis	37:48	arg1	Virus					50:54	Eastern Equine Encephalitis Virus	22:54	Eastern Equine Encephalitis Virus	22:54	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	4	33	theme	envelope	438:445	arg1	E2					455:456	The envelope protein E2	434:456	The envelope protein E2	434:456	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	0	34	theme	Equine	30:35	arg1	Encephalitis					37:48	Eastern Equine Encephalitis	22:48	Eastern Equine Encephalitis Virus	22:54	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.					
30540945	6	35	from	proteins	839:846	arg1	changes					793:799	pH changes	790:799	pH changes	790:799	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	6	35	from	proteins	839:846	arg1	dissociation					812:823	capsid dissociation	805:823	capsid dissociation from envelope proteins	805:846	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	4	36	theme	heparan	515:521	arg1	sulfate					523:529	the cellular attachment factor heparan sulfate	484:529	the cellular attachment factor heparan sulfate	484:529	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	8	37	theme	host	1119:1122	arg1	interactions					1129:1140	EEEV host cell interactions	1114:1140	EEEV host cell interactions	1114:1140	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	2	38	theme	microscopy	237:246	arg1	structure					258:266	a 4.4-Å cryoelectron microscopy (cryo-EM) structure	216:266	a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans	216:366	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	6	39	theme	viral	756:760	arg1	entry					762:766	viral entry	756:766	viral entry	756:766	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	2	40	theme	fatal	339:343	arg1	encephalitis					345:356	fatal encephalitis	339:356	fatal encephalitis	339:356	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	3	41	theme	viral	405:409	arg1	entry					411:415	viral entry	405:415	viral entry into host cells	405:431	Our analysis provides insights into viral entry into host cells.					
30540945	8	42	theme	EEEV	1114:1117	arg1	interactions					1129:1140	EEEV host cell interactions	1114:1140	EEEV host cell interactions	1114:1140	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	2	43	theme	4.4-Å	218:222	arg1	cryo-EM					249:255	cryo-EM	249:255	cryo-EM	249:255	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	2	43	theme	4.4-Å	218:222	arg1	microscopy					237:246	4.4-Å cryoelectron microscopy	218:246	a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans	216:366	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	2	44	theme	cryoelectron	224:235	arg1	cryo-EM					249:255	cryo-EM	249:255	cryo-EM	249:255	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	2	44	theme	cryoelectron	224:235	arg1	microscopy					237:246	4.4-Å cryoelectron microscopy	218:246	a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans	216:366	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	7	45	theme	binding	901:907	arg1	site					909:912	a viral RNA genome binding site	882:912	a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation	882:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	5	46	theme	immune	678:683	arg1	system					685:690	the immune system	674:690	the immune system	674:690	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	6	47	theme	envelope	830:837	arg1	proteins					839:846	envelope proteins	830:846	envelope proteins	830:846	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	2	48	theme	eastern	271:277	arg1	encephalitis					286:297	eastern equine encephalitis	271:297	eastern equine encephalitis virus (EEEV)	271:310	Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans.					
30540945	5	49	theme	cryptic	550:556	arg1	glycan					561:566	a cryptic E2 glycan	548:566	a cryptic E2 glycan	548:566	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	1	50	theme	enveloped	141:149	arg1	Alphaviruses					124:135	Alphaviruses	124:135	Alphaviruses	124:135	Alphaviruses are enveloped pathogens that cause arthritis and encephalitis.					
30540945	1	50	theme	enveloped	141:149	arg1	pathogens					151:159	enveloped pathogens	141:159	enveloped pathogens that cause arthritis and encephalitis	141:197	Alphaviruses are enveloped pathogens that cause arthritis and encephalitis.					
30540945	7	51	theme	genome	960:965	arg1	translation					967:977	viral genome translation	954:977	viral genome translation	954:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	8	52	theme	vaccine	1178:1184	arg1	design					1186:1191	vaccine design	1178:1191	vaccine design	1178:1191	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	5	53	theme	system	685:690	arg1	cells					644:648	lectin-expressing myeloid lineage cells	610:648	lectin-expressing myeloid lineage cells	610:648	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	5	53	theme	system	685:690	arg1	sentinels					661:669	sentinels	661:669	sentinels of the immune system	661:690	The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system.					
30540945	7	54	theme	RNA	890:892	arg1	site					909:912	a viral RNA genome binding site	882:912	a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation	882:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	8	55	theme	protective	1146:1155	arg1	epitopes					1157:1164	protective epitopes	1146:1164	protective epitopes relevant to vaccine design	1146:1191	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
30540945	7	56	theme	genome	989:994	arg1	release					996:1002	genome release	989:1002	genome release	989:1002	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	7	57	theme	genome	894:899	arg1	site					909:912	a viral RNA genome binding site	882:912	a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation	882:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	7	58	theme	ribosome	928:935	arg1	site					945:948	a ribosome binding site	926:948	a ribosome binding site for viral genome translation	926:977	The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release.					
30540945	4	59	theme	cellular	488:495	arg1	factor					508:513	cellular attachment factor	488:513	the cellular attachment factor heparan sulfate	484:529	The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate.					
30540945	6	60	theme	core	722:725	arg1	release					727:733	nucleocapsid core release	709:733	nucleocapsid core release	709:733	A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins.					
30540945	8	61	theme	cell	1124:1127	arg1	interactions					1129:1140	EEEV host cell interactions	1114:1140	EEEV host cell interactions	1114:1140	Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.					
31674785	4	0	theme	anomeric	915:922	arg1	steps					937:941	no anomeric manipulation steps	912:941	no anomeric manipulation steps between glycosylations	912:964	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	0	1	theme	Chemoselective	90:103	arg1	Glycosylation					105:117	Iterative Chemoselective Glycosylation	80:117	Iterative Chemoselective Glycosylation	80:117	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	1	2	theme	conditions	364:373	arg1	number					329:334	a number	327:334	a number of biological processes and conditions including cancer and viral infection	327:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	2	theme	conditions	364:373	arg1	cancer					385:390	cancer	385:390	cancer	385:390	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	2	theme	conditions	364:373	arg1	infection					402:410	viral infection	396:410	viral infection	396:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	4	3	theme	general	720:726	arg1	approach					728:735	a general approach	718:735	a general approach	718:735	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	0	4	theme	Conformationally	130:145	arg1	Thioglycosides					183:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	4	5	theme	thioglycoside	805:817	arg1	blocks					828:833	only shelf-stable thioglycoside building blocks	787:833	only shelf-stable thioglycoside building blocks	787:833	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	4	6	theme	manipulation	924:935	arg1	steps					937:941	no anomeric manipulation steps	912:941	no anomeric manipulation steps between glycosylations	912:964	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	4	7	theme	shelf-stable	792:803	arg1	blocks					828:833	only shelf-stable thioglycoside building blocks	787:833	only shelf-stable thioglycoside building blocks	787:833	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	3	8	theme	new	552:554	arg1	approach					566:573	a new synthetic approach	550:573	a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif	550:693	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	0	9	theme	Disarmed	147:154	arg1	Thioglycosides					183:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	1	10	theme	viral	396:400	arg1	infection					402:410	viral infection	396:410	viral infection	396:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	3	11	theme	synthetic	556:564	arg1	approach					566:573	a new synthetic approach	550:573	a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif	550:693	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	2	12	theme	biological	513:522	arg1	study					524:528	a biological study	511:528	a biological study	511:528	Chemical synthesis of HS and DS is required to generate structurally defined oligosaccharides for a biological study.					
31674785	2	13	theme	Chemical	413:420	arg1	synthesis					422:430	Chemical synthesis	413:430	Chemical synthesis of HS and DS	413:443	Chemical synthesis of HS and DS is required to generate structurally defined oligosaccharides for a biological study.					
31674785	1	14	theme	dermatan	224:231	arg1	sulfate					207:213	Heparan sulfate	199:213	Heparan sulfate (HS)	199:218	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	14	theme	dermatan	224:231	arg1	DS					242:243	DS	242:243	DS	242:243	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	14	theme	dermatan	224:231	arg1	glycosaminoglycans					277:294	l-iduronic acid containing glycosaminoglycans	250:294	l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection	250:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	14	theme	dermatan	224:231	arg1	sulfate					233:239	dermatan sulfate	224:239	dermatan sulfate (DS)	224:244	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	0	15	theme	Sulfate-	21:28	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation	0:117	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	0	16	theme	l-Iduronic	164:173	arg1	Thioglycosides					183:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	0	17	theme	Heparan	13:19	arg1	Sulfate-					21:28	Heparan Sulfate-	13:28	Heparan Sulfate-	13:28	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	3	18	theme	l-ido	675:679	arg1	motif					689:693	a disarmed [2.2.2] l-ido lactone motif	656:693	a disarmed [2.2.2] l-ido lactone motif	656:693	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	2	19	theme	DS	442:443	arg1	synthesis					422:430	Chemical synthesis	413:430	Chemical synthesis of HS and DS	413:443	Chemical synthesis of HS and DS is required to generate structurally defined oligosaccharides for a biological study.					
31674785	0	20	theme	Dermatan	34:41	arg1	Oligosaccharides					59:74	Dermatan Sulfate-Related Oligosaccharides	34:74	Dermatan Sulfate-Related Oligosaccharides	34:74	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	3	21	theme	lactone	681:687	arg1	motif					689:693	a disarmed [2.2.2] l-ido lactone motif	656:693	a disarmed [2.2.2] l-ido lactone motif	656:693	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	0	22	theme	Lactone	175:181	arg1	Thioglycosides					183:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides	130:196	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	2	23	theme	HS	435:436	arg1	synthesis					422:430	Chemical synthesis	413:430	Chemical synthesis of HS and DS	413:443	Chemical synthesis of HS and DS is required to generate structurally defined oligosaccharides for a biological study.					
31674785	0	24	theme	Oligosaccharides	59:74	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation	0:117	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	4	25	theme	iterative-reducing	741:758	arg1	extension					770:778	iterative-reducing end chain extension	741:778	iterative-reducing end chain extension	741:778	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	0	26	theme	Sulfate-Related	43:57	arg1	Oligosaccharides					59:74	Dermatan Sulfate-Related Oligosaccharides	34:74	Dermatan Sulfate-Related Oligosaccharides	34:74	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	2	27	theme	defined	482:488	arg1	oligosaccharides					490:505	structurally defined oligosaccharides	469:505	structurally defined oligosaccharides	469:505	Chemical synthesis of HS and DS is required to generate structurally defined oligosaccharides for a biological study.					
31674785	3	28	theme	chemoselective	611:624	arg1	glycosylation					626:638	chemoselective glycosylation	611:638	chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif	611:693	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	1	29	theme	biological	339:348	arg1	processes					350:358	biological processes	339:358	biological processes	339:358	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	29	theme	biological	339:348	arg1	cancer					385:390	cancer	385:390	cancer	385:390	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	29	theme	biological	339:348	arg1	infection					402:410	viral infection	396:410	viral infection	396:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	4	30	theme	chain	764:768	arg1	extension					770:778	iterative-reducing end chain extension	741:778	iterative-reducing end chain extension	741:778	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	4	31	theme	building	819:826	arg1	blocks					828:833	only shelf-stable thioglycoside building blocks	787:833	only shelf-stable thioglycoside building blocks	787:833	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	4	32	theme	end	760:762	arg1	extension					770:778	iterative-reducing end chain extension	741:778	iterative-reducing end chain extension	741:778	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	1	33	theme	l-iduronic	250:259	arg1	sulfate					207:213	Heparan sulfate	199:213	Heparan sulfate (HS)	199:218	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	33	theme	l-iduronic	250:259	arg1	GAGs					297:300	GAGs	297:300	GAGs	297:300	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	33	theme	l-iduronic	250:259	arg1	sulfate					233:239	dermatan sulfate	224:239	dermatan sulfate (DS)	224:244	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	33	theme	l-iduronic	250:259	arg1	glycosaminoglycans					277:294	l-iduronic acid containing glycosaminoglycans	250:294	l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection	250:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	0	34	theme	Iterative	80:88	arg1	Glycosylation					105:117	Iterative Chemoselective Glycosylation	80:117	Iterative Chemoselective Glycosylation	80:117	Synthesis of Heparan Sulfate- and Dermatan Sulfate-Related Oligosaccharides via Iterative Chemoselective Glycosylation Exploiting Conformationally Disarmed [2.2.2] l-Iduronic Lactone Thioglycosides.					
31674785	4	35	theme	conformational	849:862	arg1	switch					864:869	a conformational switch	847:869	a conformational switch	847:869	The strategy provides a general approach for iterative-reducing end chain extension, using only shelf-stable thioglycoside building blocks, exploiting a conformational switch to control reactivity, and thus requires no anomeric manipulation steps between glycosylations.					
31674785	3	36	dep	HS	578:579	arg1	oligosaccharides					588:603	oligosaccharides	588:603	oligosaccharides	588:603	Herein, we present a new synthetic approach to HS and DS oligosaccharides using chemoselective glycosylation which relies on a disarmed [2.2.2] l-ido lactone motif.					
31674785	1	37	theme	acid	261:264	arg1	sulfate					207:213	Heparan sulfate	199:213	Heparan sulfate (HS)	199:218	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	37	theme	acid	261:264	arg1	GAGs					297:300	GAGs	297:300	GAGs	297:300	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	37	theme	acid	261:264	arg1	sulfate					233:239	dermatan sulfate	224:239	dermatan sulfate (DS)	224:244	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	37	theme	acid	261:264	arg1	glycosaminoglycans					277:294	l-iduronic acid containing glycosaminoglycans	250:294	l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection	250:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	38	theme	processes	350:358	arg1	number					329:334	a number	327:334	a number of biological processes and conditions including cancer and viral infection	327:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	38	theme	processes	350:358	arg1	cancer					385:390	cancer	385:390	cancer	385:390	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	38	theme	processes	350:358	arg1	infection					402:410	viral infection	396:410	viral infection	396:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	39	theme	Heparan	199:205	arg1	HS					216:217	HS	216:217	HS	216:217	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	39	theme	Heparan	199:205	arg1	sulfate					207:213	Heparan sulfate	199:213	Heparan sulfate (HS)	199:218	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	39	theme	Heparan	199:205	arg1	sulfate					233:239	dermatan sulfate	224:239	dermatan sulfate (DS)	224:244	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	39	theme	Heparan	199:205	arg1	glycosaminoglycans					277:294	l-iduronic acid containing glycosaminoglycans	250:294	l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection	250:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	40	theme	containing	266:275	arg1	sulfate					207:213	Heparan sulfate	199:213	Heparan sulfate (HS)	199:218	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	40	theme	containing	266:275	arg1	GAGs					297:300	GAGs	297:300	GAGs	297:300	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	40	theme	containing	266:275	arg1	sulfate					233:239	dermatan sulfate	224:239	dermatan sulfate (DS)	224:244	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
31674785	1	40	theme	containing	266:275	arg1	glycosaminoglycans					277:294	l-iduronic acid containing glycosaminoglycans	250:294	l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection	250:410	Heparan sulfate (HS) and dermatan sulfate (DS) are l-iduronic acid containing glycosaminoglycans (GAGs) which are implicated in a number of biological processes and conditions including cancer and viral infection.					
30427198	3	0	theme	of	754:755	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	1	theme	glycosidase	373:383	arg1	family					385:390	the GH99 glycosidase family	364:390	the GH99 glycosidase family	364:390	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	0	2	theme	Inhibitor	73:81	arg1	Synthesis					50:58	Synthesis	50:58	Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase	50:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	2	3	theme	GH99	368:371	arg1	family					385:390	the GH99 glycosidase family	364:390	the GH99 glycosidase family	364:390	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	0	4	theme	GH99	93:96	arg1	Endo-α-mannosidase					98:115	Family GH99 Endo-α-mannosidase	86:115	Family GH99 Endo-α-mannosidase	86:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	3	5	theme	aziridine	696:704	arg1	nitrogen					706:713	the aziridine nitrogen	692:713	the aziridine nitrogen	692:713	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	6	theme	α-1,3-linked	293:304	arg1	carbasugar					315:324	an α-1,3-linked glycosyl carbasugar	290:324	an α-1,3-linked glycosyl carbasugar	290:324	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	0	7	theme	Family	86:91	arg1	Endo-α-mannosidase					98:115	Family GH99 Endo-α-mannosidase	86:115	Family GH99 Endo-α-mannosidase	86:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	3	8	theme	catalytic	734:742	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	9	theme	carbasugar	544:553	arg1	inhibitors					616:625	reasonably potent endo-α-mannosidase inhibitors	579:625	reasonably potent endo-α-mannosidase inhibitors	579:625	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	9	theme	carbasugar	544:553	arg1	α-aziridines					555:566	glucosyl carbasugar α-aziridines	535:566	glucosyl carbasugar α-aziridines	535:566	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	10	theme	potent	590:595	arg1	inhibitors					616:625	reasonably potent endo-α-mannosidase inhibitors	579:625	reasonably potent endo-α-mannosidase inhibitors	579:625	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	10	theme	potent	590:595	arg1	α-aziridines					555:566	glucosyl carbasugar α-aziridines	535:566	glucosyl carbasugar α-aziridines	535:566	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	11	theme	acid/base	744:752	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	0	12	theme	Endo-α-mannosidase	98:115	arg1	Inhibitor					73:81	a Bespoke Inhibitor	63:81	a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase	63:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	2	13	theme	α-1,4-disaccharide	266:283	arg1	conversion					249:258	conversion	249:258	conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar	249:324	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	3	14	with	interactions	676:687	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	15	theme	conserved	724:732	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	16	theme	1,2-anhydrosugar	440:455	arg1	intermediate					467:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	3	17	theme	enzyme	761:766	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	18	theme	interactions	676:687	arg1	virtue					638:643	virtue	638:643	virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site	638:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	1	19	theme	enzyme	188:193	arg1	inhibitors					195:204	enzyme inhibitors	188:204	enzyme inhibitors	188:204	Understanding the enzyme reaction mechanism can lead to the design of enzyme inhibitors.					
30427198	3	20	theme	active	768:773	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	21	from	family	385:390	arg1	endo-α-mannosidase					340:357	the endo-α-mannosidase	336:357	the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate	336:478	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	1	22	theme	inhibitors	195:204	arg1	design					178:183	the design	174:183	the design of enzyme inhibitors	174:204	Understanding the enzyme reaction mechanism can lead to the design of enzyme inhibitors.					
30427198	2	23	link	α-1,3-linked	293:304	arg1	carbasugar					315:324	an α-1,3-linked glycosyl carbasugar	290:324	an α-1,3-linked glycosyl carbasugar	290:324	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	3	24	theme	shape	654:658	arg1	mimicry					660:666	their shape mimicry	648:666	their shape mimicry	648:666	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	25	theme	glycosyl	306:313	arg1	carbasugar					315:324	an α-1,3-linked glycosyl carbasugar	290:324	an α-1,3-linked glycosyl carbasugar	290:324	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	2	26	used	used	235:238	arg2	rearrangement					217:229	A Claisen rearrangement	207:229	A Claisen rearrangement	207:229	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	3	27	theme	mimicry	660:666	arg1	virtue					638:643	virtue	638:643	virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site	638:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	28	theme	endo-α-mannosidase	597:614	arg1	inhibitors					616:625	reasonably potent endo-α-mannosidase inhibitors	579:625	reasonably potent endo-α-mannosidase inhibitors	579:625	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	28	theme	endo-α-mannosidase	597:614	arg1	α-aziridines					555:566	glucosyl carbasugar α-aziridines	535:566	glucosyl carbasugar α-aziridines	535:566	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	29	with	mimicry	660:666	arg1	site					775:778	the conserved catalytic acid/base of the enzyme active site	720:778	the conserved catalytic acid/base of the enzyme active site	720:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	2	30	theme	"	465:465	arg1	intermediate					467:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	2	31	theme	epoxide	458:464	arg1	intermediate					467:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	a 1,2-anhydrosugar "epoxide" intermediate	438:478	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	2	32	theme	Claisen	209:215	arg1	rearrangement					217:229	A Claisen rearrangement	207:229	A Claisen rearrangement	207:229	A Claisen rearrangement was used to allow conversion of an α-1,4-disaccharide into an α-1,3-linked glycosyl carbasugar to target the endo-α-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate.					
30427198	0	33	dep	Carbasugar	38:47	arg1	1,3-Glycosyl					25:36	1,3-Glycosyl	25:36	1,3-Glycosyl	25:36	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	0	33	dep	Carbasugar	38:47	arg1	Synthesis					50:58	Synthesis	50:58	Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase	50:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	3	34	theme	X-ray	495:499	arg1	crystallography					501:515	X-ray crystallography	495:515	X-ray crystallography	495:515	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	0	35	dep	1,3-Glycosyl	25:36	arg1	1,4-Disaccharide					5:20	1,4-Disaccharide	5:20	1,4-Disaccharide	5:20	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	1	36	theme	enzyme	136:141	arg1	mechanism					152:160	the enzyme reaction mechanism	132:160	the enzyme reaction mechanism	132:160	Understanding the enzyme reaction mechanism can lead to the design of enzyme inhibitors.					
30427198	3	37	theme	glucosyl	535:542	arg1	inhibitors					616:625	reasonably potent endo-α-mannosidase inhibitors	579:625	reasonably potent endo-α-mannosidase inhibitors	579:625	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	37	theme	glucosyl	535:542	arg1	α-aziridines					555:566	glucosyl carbasugar α-aziridines	535:566	glucosyl carbasugar α-aziridines	535:566	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	1	38	theme	reaction	143:150	arg1	mechanism					152:160	the enzyme reaction mechanism	132:160	the enzyme reaction mechanism	132:160	Understanding the enzyme reaction mechanism can lead to the design of enzyme inhibitors.					
30427198	0	39	theme	Bespoke	65:71	arg1	Inhibitor					73:81	a Bespoke Inhibitor	63:81	a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase	63:115	From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-α-mannosidase.					
30427198	3	40	theme	nitrogen	706:713	arg1	mimicry					660:666	their shape mimicry	648:666	their shape mimicry	648:666	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30427198	3	40	theme	nitrogen	706:713	arg1	interactions					676:687	the interactions	672:687	the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site	672:778	Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar α-aziridines can act as reasonably potent endo-α-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.					
30403372	0	0	theme	trimer	73:78	arg1	recognition					24:34	Germline VRC01 antibody recognition	0:34	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.	0:115	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	2	1	theme	germline	320:327	arg1	precursors					329:338	corresponding VRC01 germline precursors	300:338	corresponding VRC01 germline precursors	300:338	Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env.					
30403372	1	2	theme	immunodeficiency	212:227	arg1	virus-1					229:235	the human immunodeficiency virus-1	202:235	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	2	theme	immunodeficiency	212:227	arg1	HIV-1					238:242	HIV-1	238:242	HIV-1	238:242	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	0	3	theme	envelope	64:71	arg1	trimer					73:78	a modified clade C HIV-1 envelope trimer	39:78	a modified clade C HIV-1 envelope trimer	39:78	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	2	4	theme	VRC01	314:318	arg1	precursors					329:338	corresponding VRC01 germline precursors	300:338	corresponding VRC01 germline precursors	300:338	Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env.					
30403372	1	5	theme	virus-1	229:235	arg1	site					186:189	the CD4-binding site	170:189	the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	170:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	5	theme	virus-1	229:235	arg1	virus-1					229:235	the human immunodeficiency virus-1	202:235	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	5	theme	virus-1	229:235	arg1	CD4BS					192:196	CD4BS	192:196	CD4BS	192:196	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	4	6	theme	trimeric	636:643	arg1	Env					650:652	a modified trimeric 426c Env	625:652	a modified trimeric 426c Env	625:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	5	7	theme	426c	868:871	arg1	core					873:876	a wild-type 426c core	856:876	a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276	856:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	3	8	theme	Immunogen	360:368	arg1	design					370:375	Immunogen design	360:375	Immunogen design	360:375	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	6	9	theme	carbohydrate	1113:1124	arg1	shortening					1126:1135	carbohydrate shortening	1113:1135	carbohydrate shortening	1113:1135	Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.					
30403372	6	10	from	Interactions	1032:1043	arg1	presence					1052:1059	the presence	1048:1059	the presence of an Asn276 oligosaccharide	1048:1088	Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.					
30403372	4	11	theme	X-ray	707:711	arg1	crystallography					713:727	X-ray crystallography	707:727	X-ray crystallography	707:727	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	0	12	theme	HIV-1	99:103	arg1	gp120					105:109	a glycosylated HIV-1 gp120	84:109	a glycosylated HIV-1 gp120 core	84:114	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	5	13	theme	variable	886:893	arg1	loops					895:899	variable loops	886:899	variable loops 1-3	886:903	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	3	14	theme	VRC01	490:494	arg1	bnAbs					496:500	mature VRC01 bnAbs	483:500	mature VRC01 bnAbs	483:500	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	3	15	theme	glycan	398:403	arg1	removal					405:411	glycan removal	398:411	glycan removal from trimeric Env constructs	398:440	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	0	16	theme	glycosylated	86:97	arg1	gp120					105:109	a glycosylated HIV-1 gp120	84:109	a glycosylated HIV-1 gp120 core	84:114	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	4	17	theme	bnAbs	544:548	arg1	elicitation					524:534	elicitation	524:534	elicitation of such bnAbs	524:548	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	5	18	theme	glycan	938:943	arg1	absence					925:931	absence	925:931	absence	925:931	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	18	theme	glycan	938:943	arg1	presence					912:919	presence	912:919	presence	912:919	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	0	19	theme	VRC01	9:13	arg1	recognition					24:34	Germline VRC01 antibody recognition	0:34	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.	0:115	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	3	20	contain	had	450:452	arg1	design					370:375	Immunogen design	360:375	Immunogen design	360:375	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	3	20	contain	had	450:452	arg2	success					462:468	limited success	454:468	limited success in eliciting mature VRC01 bnAbs	454:500	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	4	21	theme	gp120	693:697	arg1	core					699:702	a 426c gp120 core	686:702	a 426c gp120 core	686:702	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	5	22	from	Asn276	957:962	arg1	absence					925:931	absence	925:931	absence	925:931	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	22	from	Asn276	957:962	arg1	glycan					938:943	a glycan	936:943	a glycan at position Asn276	936:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	22	from	Asn276	957:962	arg1	presence					912:919	presence	912:919	presence	912:919	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	4	23	theme	modified	627:634	arg1	Env					650:652	a modified trimeric 426c Env	625:652	a modified trimeric 426c Env	625:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	0	24	theme	Germline	0:7	arg1	recognition					24:34	Germline VRC01 antibody recognition	0:34	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.	0:115	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	4	25	theme	426c	688:691	arg1	core					699:702	a 426c gp120 core	686:702	a 426c gp120 core	686:702	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	1	26	theme	envelope	245:252	arg1	glycoprotein					254:265	envelope glycoprotein	245:265	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	26	theme	envelope	245:252	arg1	Env					268:270	Env	268:270	Env	268:270	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	0	27	theme	core	111:114	arg1	recognition					24:34	Germline VRC01 antibody recognition	0:34	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.	0:115	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	0	28	gly	glycosylated	86:97	arg1	gp120					105:109	a glycosylated HIV-1 gp120	84:109	a glycosylated HIV-1 gp120 core	84:114	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	1	29	theme	CD4-binding	174:184	arg1	site					186:189	the CD4-binding site	170:189	the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	170:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	29	theme	CD4-binding	174:184	arg1	virus-1					229:235	the human immunodeficiency virus-1	202:235	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	29	theme	CD4-binding	174:184	arg1	CD4BS					192:196	CD4BS	192:196	CD4BS	192:196	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	0	30	theme	antibody	15:22	arg1	recognition					24:34	Germline VRC01 antibody recognition	0:34	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.	0:115	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	0	31	theme	gp120	105:109	arg1	core					111:114	a glycosylated HIV-1 gp120 core	84:114	a glycosylated HIV-1 gp120 core	84:114	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	5	32	dep	presence	912:919	arg1	the					908:910	the	908:910	the	908:910	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	33	theme	wild-type	858:866	arg1	core					873:876	a wild-type 426c core	856:876	a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276	856:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	4	34	theme	cryo-electron	657:669	arg1	microscopy					671:680	cryo-electron microscopy	657:680	cryo-electron microscopy	657:680	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	6	35	theme	oligosaccharide	1074:1088	arg1	presence					1052:1059	the presence	1048:1059	the presence of an Asn276 oligosaccharide	1048:1088	Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.					
30403372	4	36	theme	VRC01	603:607	arg1	precursor					590:598	the inferred germline precursor	568:598	the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env	568:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	4	37	from	precursor	590:598	arg1	complex					612:618	complex	612:618	complex with a modified trimeric 426c Env	612:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	5	38	theme	VRC01	814:818	arg1	antibodies					829:838	VRC01 germline antibodies	814:838	VRC01 germline antibodies	814:838	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	4	39	theme	such	539:542	arg1	bnAbs					544:548	such bnAbs	539:548	such bnAbs	539:548	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	5	40	theme	latter	974:979	arg1	form					981:984	the latter form	970:984	the latter form binding with higher affinity than the former	970:1029	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	41	theme	germline	820:827	arg1	antibodies					829:838	VRC01 germline antibodies	814:838	VRC01 germline antibodies	814:838	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	3	42	theme	trimeric	418:425	arg1	constructs					431:440	trimeric Env constructs	418:440	trimeric Env constructs	418:440	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	0	43	theme	clade	50:54	arg1	trimer					73:78	a modified clade C HIV-1 envelope trimer	39:78	a modified clade C HIV-1 envelope trimer	39:78	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	4	44	with	complex	612:618	arg1	Env					650:652	a modified trimeric 426c Env	625:652	a modified trimeric 426c Env	625:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	2	45	theme	corresponding	300:312	arg1	precursors					329:338	corresponding VRC01 germline precursors	300:338	corresponding VRC01 germline precursors	300:338	Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env.					
30403372	0	46	theme	modified	41:48	arg1	trimer					73:78	a modified clade C HIV-1 envelope trimer	39:78	a modified clade C HIV-1 envelope trimer	39:78	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	5	47	from	presence	912:919	arg1	Asn276					957:962	position Asn276	948:962	position Asn276	948:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	3	48	theme	limited	454:460	arg1	success					462:468	limited success	454:468	limited success in eliciting mature VRC01 bnAbs	454:500	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	5	49	theme	higher	999:1004	arg1	affinity					1006:1013	higher affinity	999:1013	higher affinity than the former	999:1029	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	5	50	theme	position	948:955	arg1	Asn276					957:962	position Asn276	948:962	position Asn276	948:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	1	51	gly	glycoprotein	254:265	arg1	glycoprotein					254:265	envelope glycoprotein	245:265	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	51	gly	glycoprotein	254:265	arg1	Env					268:270	Env	268:270	Env	268:270	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	6	52	theme	Asn276	1067:1072	arg1	oligosaccharide					1074:1088	an Asn276 oligosaccharide	1064:1088	an Asn276 oligosaccharide	1064:1088	Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.					
30403372	5	53	from	absence	925:931	arg1	Asn276					957:962	position Asn276	948:962	position Asn276	948:962	Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former.					
30403372	3	54	from	constructs	431:440	arg1	removal					405:411	glycan removal	398:411	glycan removal from trimeric Env constructs	398:440	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	4	55	theme	biolayer	730:737	arg1	interferometry					739:752	biolayer interferometry	730:752	biolayer interferometry	730:752	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	2	56	theme	mature	281:286	arg1	antibodies					288:297	mature antibodies	281:297	mature antibodies	281:297	Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env.					
30403372	0	57	theme	HIV-1	58:62	arg1	trimer					73:78	a modified clade C HIV-1 envelope trimer	39:78	a modified clade C HIV-1 envelope trimer	39:78	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	1	58	dep	virus-1	229:235	arg1	glycoprotein					254:265	envelope glycoprotein	245:265	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	58	dep	virus-1	229:235	arg1	Env					268:270	Env	268:270	Env	268:270	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	3	59	theme	Env	427:429	arg1	constructs					431:440	trimeric Env constructs	418:440	trimeric Env constructs	418:440	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	4	60	theme	426c	645:648	arg1	Env					650:652	a modified trimeric 426c Env	625:652	a modified trimeric 426c Env	625:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	3	61	theme	mature	483:488	arg1	bnAbs					496:500	mature VRC01 bnAbs	483:500	mature VRC01 bnAbs	483:500	Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs.					
30403372	4	62	theme	germline	581:588	arg1	precursor					590:598	the inferred germline precursor	568:598	the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env	568:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	0	63	theme	C	56:56	arg1	trimer					73:78	a modified clade C HIV-1 envelope trimer	39:78	a modified clade C HIV-1 envelope trimer	39:78	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.					
30403372	4	64	theme	inferred	572:579	arg1	precursor					590:598	the inferred germline precursor	568:598	the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env	568:652	To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics.					
30403372	1	65	theme	neutralizing	131:142	arg1	bnAbs					156:160	bnAbs	156:160	bnAbs	156:160	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	65	theme	neutralizing	131:142	arg1	antibodies					144:153	VRC01 broadly neutralizing antibodies	117:153	VRC01 broadly neutralizing antibodies (bnAbs)	117:161	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	66	theme	human	206:210	arg1	virus-1					229:235	the human immunodeficiency virus-1	202:235	the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env)	202:271	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
30403372	1	66	theme	human	206:210	arg1	HIV-1					238:242	HIV-1	238:242	HIV-1	238:242	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4BS) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env).					
29790762	0	0	theme	B	100:100	arg1	Fragment					59:66	the Branched Trisaccharide Fragment	32:66	the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B	32:100	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	4	1	theme	total	745:749	arg1	synthesis					751:759	the total synthesis	741:759	the total synthesis of the antibiotic saccharomicin B	741:793	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	0	2	theme	Saccharomicin	86:98	arg1	B					100:100	the Antibiotic Saccharomicin B	71:100	the Antibiotic Saccharomicin B	71:100	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	2	3	theme	d-fucose	317:324	arg1	acceptor					326:333	the d-fucose acceptor	313:333	the d-fucose acceptor	313:333	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	3	4	theme	single	625:630	arg1	trisaccharide					606:618	the trisaccharide	602:618	the trisaccharide	602:618	Following deprotection, l-digitoxose was installed using the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer.					
29790762	3	4	theme	single	625:630	arg1	α-anomer					632:639	a single α-anomer	623:639	a single α-anomer	623:639	Following deprotection, l-digitoxose was installed using the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer.					
29790762	1	5	theme	saccharomicin	212:224	arg1	B					226:226	saccharomicin B	212:226	saccharomicin B	212:226	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	6	dep	trisaccharide	137:149	arg1	-β-d-Fuc					185:192	-β-d-Fuc	185:192	-β-d-Fuc	185:192	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	3	7	theme	thioether-activation	563:582	arg1	method					584:589	the AgPF6/TTBP thioether-activation method	548:589	the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer	548:639	Following deprotection, l-digitoxose was installed using the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer.					
29790762	0	8	theme	Fragment	59:66	arg1	Synthesis					19:27	Reagent-Controlled Synthesis	0:27	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.	0:101	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	0	9	theme	Reagent-Controlled	0:17	arg1	Synthesis					19:27	Reagent-Controlled Synthesis	0:27	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.	0:101	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	2	10	theme	mediated	455:462	arg1	glycosylation					476:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	4	11	theme	B	793:793	arg1	synthesis					751:759	the total synthesis	741:759	the total synthesis of the antibiotic saccharomicin B	741:793	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	2	12	theme	bromide	447:453	arg1	glycosylation					476:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	4	13	theme	saccharomicin	779:791	arg1	B					793:793	the antibiotic saccharomicin B	764:793	the antibiotic saccharomicin B	764:793	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	2	14	theme	cyclopropene-1-thione/oxalyl	418:445	arg1	bromide					447:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide	387:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	1	15	theme	concise	105:111	arg1	synthesis					113:121	A concise synthesis	103:121	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B,	103:227	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	2	16	theme	2,3,4-trimethoxyphenyl	395:416	arg1	bromide					447:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide	387:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	0	17	theme	Trisaccharide	45:57	arg1	Fragment					59:66	the Branched Trisaccharide Fragment	32:66	the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B	32:100	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	2	18	theme	2,3-bis	387:393	arg1	bromide					447:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide	387:453	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	0	19	theme	Branched	36:43	arg1	Fragment					59:66	the Branched Trisaccharide Fragment	32:66	the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B	32:100	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	1	20	theme	reagent-controlled	252:269	arg1	glycosylations					283:296	reagent-controlled α-selective glycosylations	252:296	reagent-controlled α-selective glycosylations	252:296	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	21	dep	→	163:163	arg1	-[α-l-Eva-					167:176	-[α-l-Eva-	167:176	1 → 3)-[α-l-Eva-(1 → 4)	161:183	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	21	dep	→	163:163	arg1	3					165:165	3	165:165	3	165:165	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	2	22	theme	dehydrative	464:474	arg1	glycosylation					476:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation	387:488	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	1	23	theme	α-selective	271:281	arg1	glycosylations					283:296	reagent-controlled α-selective glycosylations	252:296	reagent-controlled α-selective glycosylations	252:296	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	24	theme	branched	128:135	arg1	α-l-Dig-					152:159	α-l-Dig-	152:159	α-l-Dig-	152:159	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	24	theme	branched	128:135	arg1	→					163:163	1 → 3)-[α-l-Eva-(1 → 4)	161:183	1 → 3)-[α-l-Eva-(1 → 4)	161:183	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	1	24	theme	branched	128:135	arg1	trisaccharide					137:149	a branched trisaccharide	126:149	a branched trisaccharide	126:149	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	4	25	theme	functionalized	654:667	arg1	donor					715:719	a donor	713:719	a donor	713:719	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	4	25	theme	functionalized	654:667	arg1	acceptor					728:735	an acceptor	725:735	an acceptor for the total synthesis of the antibiotic saccharomicin B	725:793	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	4	25	theme	functionalized	654:667	arg1	trisaccharide					669:681	This highly functionalized trisaccharide	642:681	This highly functionalized trisaccharide	642:681	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	1	26	theme	trisaccharide	137:149	arg1	synthesis					113:121	A concise synthesis	103:121	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B,	103:227	A concise synthesis of a branched trisaccharide, α-l-Dig-(1 → 3)-[α-l-Eva-(1 → 4)]-β-d-Fuc, corresponding to saccharomicin B, has been developed via reagent-controlled α-selective glycosylations.					
29790762	4	27	theme	antibiotic	768:777	arg1	B					793:793	the antibiotic saccharomicin B	764:793	the antibiotic saccharomicin B	764:793	This highly functionalized trisaccharide can potentially serve as both a donor and an acceptor for the total synthesis of the antibiotic saccharomicin B.					
29790762	0	28	theme	Antibiotic	75:84	arg1	B					100:100	the Antibiotic Saccharomicin B	71:100	the Antibiotic Saccharomicin B	71:100	Reagent-Controlled Synthesis of the Branched Trisaccharide Fragment of the Antibiotic Saccharomicin B.					
29790762	2	29	theme	l-	336:337	arg1	epi-vancosamine					339:353	l- epi-vancosamine	336:353	l- epi-vancosamine	336:353	Starting from the d-fucose acceptor, l- epi-vancosamine was selectively installed using 2,3-bis(2,3,4-trimethoxyphenyl)cyclopropene-1-thione/oxalyl bromide mediated dehydrative glycosylation.					
29790762	3	30	theme	AgPF6/TTBP	552:561	arg1	method					584:589	the AgPF6/TTBP thioether-activation method	548:589	the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer	548:639	Following deprotection, l-digitoxose was installed using the AgPF6/TTBP thioether-activation method to produce the trisaccharide as a single α-anomer.					
30539483	0	0	theme	Fc	78:79	arg1	Region					82:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region	34:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1	34:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	2	1	theme	posttranslational	342:358	arg1	modifications					360:372	posttranslational modifications	342:372	posttranslational modifications (PTMs)	342:379	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	2	1	theme	posttranslational	342:358	arg1	addition					410:417	the addition	406:417	the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic	406:512	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	2	1	theme	posttranslational	342:358	arg1	PTMs					375:378	PTMs	375:378	PTMs	375:378	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	1	2	theme	monoclonal	223:232	arg1	antibodies					234:243	monoclonal antibodies	223:243	monoclonal antibodies	223:243	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	0	3	theme	Region	82:87	arg1	Binding					23:29	the Binding	19:29	the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors	19:121	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	3	4	theme	Fc-fragment	678:688	arg1	therapies					690:698	Fc-fragment therapies	678:698	Fc-fragment therapies	678:698	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	3	5	theme	detailed	535:542	arg1	method					544:549	a detailed method	533:549	a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines	533:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	0	6	theme	Human	92:96	arg1	IgG1					98:101	Human IgG1	92:101	Human IgG1	92:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	3	7	gly	hyper-sialylated	577:592	arg1	Fcs					594:596	hyper-sialylated Fcs	577:596	hyper-sialylated Fcs	577:596	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	1	8	theme	crystallizable	149:162	arg1	Fc					165:166	Fc	165:166	Fc	165:166	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	1	8	theme	crystallizable	149:162	arg1	fragment					140:147	the fragment crystallizable	136:162	the fragment crystallizable (Fc) of human IgG	136:180	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	2	9	theme	therapeutic	502:512	arg1	class					493:497	this class	488:497	this class of therapeutic	488:512	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	3	10	theme	vaccines	704:711	arg1	development					650:660	the future development	639:660	the future development of new mAbs and Fc-fragment therapies and vaccines	639:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	1	11	theme	Fc-fusion-based	249:263	arg1	medicines					265:273	Fc-fusion-based medicines	249:273	Fc-fusion-based medicines	249:273	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	2	12	theme	Fc-effector	294:304	arg1	functions					306:314	Such Fc-effector functions	289:314	Such Fc-effector functions	289:314	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	2	13	theme	glycans	422:428	arg1	addition					410:417	the addition	406:417	the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic	406:512	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	2	14	theme	Such	289:292	arg1	functions					306:314	Such Fc-effector functions	289:314	Such Fc-effector functions	289:314	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	0	15	theme	IgG1	98:101	arg1	Region					82:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region	34:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1	34:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	1	16	theme	human	172:176	arg1	IgG					178:180	human IgG	172:180	human IgG	172:180	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	3	17	theme	future	643:648	arg1	development					650:660	the future development	639:660	the future development of new mAbs and Fc-fragment therapies and vaccines	639:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	3	18	theme	hyper-sialylated	577:592	arg1	Fcs					594:596	hyper-sialylated Fcs	577:596	hyper-sialylated Fcs	577:596	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	1	19	theme	IgG	178:180	arg1	Fc					165:166	Fc	165:166	Fc	165:166	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	1	19	theme	IgG	178:180	arg1	fragment					140:147	the fragment crystallizable	136:162	the fragment crystallizable (Fc) of human IgG	136:180	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	2	20	theme	functional	460:469	arg1	heterogeneity					471:483	structural and functional heterogeneity	445:483	structural and functional heterogeneity	445:483	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	0	21	theme	Glycan	106:111	arg1	Receptors					113:121	Glycan Receptors	106:121	Glycan Receptors	106:121	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	3	22	theme	Fcs	594:596	arg1	detection					564:572	the detection	560:572	the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines	560:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	0	23	theme	Hyper-Glycosylated	34:51	arg1	Region					82:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region	34:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1	34:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	2	24	theme	structural	445:454	arg1	heterogeneity					471:483	structural and functional heterogeneity	445:483	structural and functional heterogeneity	445:483	Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional heterogeneity to this class of therapeutic.					
30539483	3	25	theme	new	665:667	arg1	mAbs					669:672	new mAbs	665:672	new mAbs	665:672	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	3	26	theme	mAbs	669:672	arg1	development					650:660	the future development	639:660	the future development of new mAbs and Fc-fragment therapies and vaccines	639:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	1	27	theme	improved	192:199	arg1	functions					210:218	improved effector functions	192:218	improved effector functions	192:218	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	0	28	theme	Crystallizable	62:75	arg1	Region					82:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region	34:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1	34:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	1	29	theme	effector	201:208	arg1	functions					210:218	improved effector functions	192:218	improved effector functions	192:218	Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines.					
30539483	0	30	theme	Fragment	53:60	arg1	Region					82:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region	34:87	Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1	34:101	A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.					
30539483	3	31	theme	therapies	690:698	arg1	development					650:660	the future development	639:660	the future development of new mAbs and Fc-fragment therapies and vaccines	639:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30539483	3	32	theme	glycan	601:606	arg1	receptors					608:616	glycan receptors	601:616	glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines	601:711	Here, we describe a detailed method to allow the detection of hyper-sialylated Fcs to glycan receptors that will facilitate the future development of new mAbs and Fc-fragment therapies and vaccines.					
30888190	0	0	theme	Streptococcus	60:72	arg1	Polysaccharide					74:87	Type VII Group B Streptococcus Polysaccharide	43:87	Type VII Group B Streptococcus Polysaccharide	43:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	1	1	theme	hexasaccharide	131:144	arg1	unit					156:159	the hexasaccharide repeating unit	127:159	the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide	127:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	2	theme	B	58:58	arg1	Streptococcus					60:72	Type VII Group B Streptococcus	43:72	Type VII Group B Streptococcus Polysaccharide	43:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	1	3	theme	repeating	146:154	arg1	unit					156:159	the hexasaccharide repeating unit	127:159	the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide	127:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	1	4	theme	one-pot	237:243	arg1	strategy					259:266	a one-pot glycosylation strategy	235:266	a one-pot glycosylation strategy	235:266	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	3	5	theme	new	562:564	arg1	method					597:602	a new stereospecific α-glucosylation method	560:602	a new stereospecific α-glucosylation method	560:602	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	1	6	theme	glycosylation	245:257	arg1	strategy					259:266	a one-pot glycosylation strategy	235:266	a one-pot glycosylation strategy	235:266	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	7	theme	Polysaccharide	74:87	arg1	Unit					35:38	the Repeating Unit	21:38	the Repeating Unit of Type VII Group B Streptococcus Polysaccharide	21:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	3	8	theme	stereospecific	566:579	arg1	method					597:602	a new stereospecific α-glucosylation method	560:602	a new stereospecific α-glucosylation method	560:602	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	3	9	theme	excellent	507:515	arg1	stereoselectivity					517:533	excellent stereoselectivity	507:533	excellent stereoselectivity	507:533	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	2	10	theme	dual	413:416	arg1	glycosylations					418:431	one-pot dual glycosylations	405:431	one-pot dual glycosylations with the side-chain trisaccharide donor	405:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	1	11	theme	unit	156:159	arg1	synthesis					114:122	The first synthesis	104:122	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide	104:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	2	12	with	glycosylations	418:431	arg1	donor					467:471	the side-chain trisaccharide donor	438:471	the side-chain trisaccharide donor	438:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	2	13	theme	side-chain	442:451	arg1	donor					467:471	the side-chain trisaccharide donor	438:471	the side-chain trisaccharide donor	438:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	2	14	theme	trisaccharide	453:465	arg1	donor					467:471	the side-chain trisaccharide donor	438:471	the side-chain trisaccharide donor	438:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	1	15	theme	type	164:167	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	16	theme	One-Pot	0:6	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide	0:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	2	17	theme	unit	297:300	arg1	dimer					273:277	The dimer	269:277	The dimer of this repeating unit	269:300	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	1	18	theme	VII	169:171	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	3	19	theme	glycosylation	478:490	arg1	reactions					492:500	All glycosylation reactions	474:500	All glycosylation reactions	474:500	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	2	20	theme	repeating	287:295	arg1	unit					297:300	this repeating unit	282:300	this repeating unit	282:300	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	1	21	theme	group	173:177	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	3	22	theme	method	597:602	arg1	effect					644:649	the neighboring group participation effect	608:649	the neighboring group participation effect for 1,2- trans glycosylations	608:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	3	22	theme	method	597:602	arg1	advantage					547:555	advantage	547:555	advantage of a new stereospecific α-glucosylation method	547:602	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	1	23	theme	B	179:179	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	24	theme	Unit	35:38	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide	0:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	0	24	theme	Unit	35:38	arg1	Dimer					97:101	the Dimer	93:101	the Dimer	93:101	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	2	25	theme	one-pot	405:411	arg1	glycosylations					418:431	one-pot dual glycosylations	405:431	one-pot dual glycosylations with the side-chain trisaccharide donor	405:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	3	26	theme	participation	630:642	arg1	effect					644:649	the neighboring group participation effect	608:649	the neighboring group participation effect for 1,2- trans glycosylations	608:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	2	27	theme	backbone	363:370	arg1	hexasaccharide					372:385	the backbone hexasaccharide	359:385	the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor	359:471	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	1	28	theme	Streptococcus	181:193	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	29	theme	Repeating	25:33	arg1	Unit					35:38	the Repeating Unit	21:38	the Repeating Unit of Type VII Group B Streptococcus Polysaccharide	21:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	1	30	theme	capsular	195:202	arg1	polysaccharide					204:217	type VII group B Streptococcus capsular polysaccharide	164:217	type VII group B Streptococcus capsular polysaccharide	164:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	31	theme	Type	43:46	arg1	Streptococcus					60:72	Type VII Group B Streptococcus	43:72	Type VII Group B Streptococcus Polysaccharide	43:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	3	32	theme	neighboring	612:622	arg1	effect					644:649	the neighboring group participation effect	608:649	the neighboring group participation effect for 1,2- trans glycosylations	608:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	2	33	theme	one-pot	326:332	arg1	glycosylation					334:346	one-pot glycosylation	326:346	one-pot glycosylation	326:346	The dimer of this repeating unit was also synthesized by one-pot glycosylation to achieve the backbone hexasaccharide first followed by one-pot dual glycosylations with the side-chain trisaccharide donor.					
30888190	3	34	theme	group	624:628	arg1	effect					644:649	the neighboring group participation effect	608:649	the neighboring group participation effect for 1,2- trans glycosylations	608:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	3	35	theme	trans	660:664	arg1	glycosylations					666:679	1,2- trans glycosylations	655:679	1,2- trans glycosylations	655:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	1	36	theme	first	108:112	arg1	synthesis					114:122	The first synthesis	104:122	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide	104:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	3	37	theme	α-glucosylation	581:595	arg1	method					597:602	a new stereospecific α-glucosylation method	560:602	a new stereospecific α-glucosylation method	560:602	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30888190	1	38	theme	polysaccharide	204:217	arg1	unit					156:159	the hexasaccharide repeating unit	127:159	the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide	127:217	The first synthesis of the hexasaccharide repeating unit of type VII group B Streptococcus capsular polysaccharide was achieved by a one-pot glycosylation strategy.					
30888190	0	39	theme	Group	52:56	arg1	Streptococcus					60:72	Type VII Group B Streptococcus	43:72	Type VII Group B Streptococcus Polysaccharide	43:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	0	40	theme	VII	48:50	arg1	Streptococcus					60:72	Type VII Group B Streptococcus	43:72	Type VII Group B Streptococcus Polysaccharide	43:87	One-Pot Synthesis of the Repeating Unit of Type VII Group B Streptococcus Polysaccharide and the Dimer.					
30888190	3	41	theme	1,2-	655:658	arg1	glycosylations					666:679	1,2- trans glycosylations	655:679	1,2- trans glycosylations	655:679	All glycosylation reactions gave excellent stereoselectivity, which took advantage of a new stereospecific α-glucosylation method and the neighboring group participation effect for 1,2- trans glycosylations.					
30342133	0	0	theme	processing	91:100	arg1	by-products					102:112	sesame processing by-products	84:112	sesame processing by-products: Functional and physico-chemical properties	84:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	9	1	theme	plant	1393:1397	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	1	theme	plant	1393:1397	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	7	2	theme	secondary	1136:1144	arg1	structures					1146:1155	the secondary structures	1132:1155	the secondary structures	1132:1155	Circular dichroism spectroscopy showed that the secondary structures were changed significantly by conjugation.					
30342133	9	3	theme	potential	1361:1369	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	3	theme	potential	1361:1369	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	4	theme	milled	256:261	arg1	sesame					263:268	dehulled, roasted and milled sesame	234:268	dehulled, roasted and milled sesame	234:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	0	5	theme	sesame	84:89	arg1	by-products					102:112	sesame processing by-products	84:112	sesame processing by-products: Functional and physico-chemical properties	84:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	9	6	theme	Ardeh	1336:1340	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	6	theme	Ardeh	1336:1340	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	7	theme	sesame	263:268	arg1	processing					220:229	processing	220:229	processing	220:229	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	7	theme	sesame	263:268	arg1	extraction					205:214	oil extraction	201:214	oil extraction	201:214	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	5	8	theme	Fourier	731:737	arg1	analysis					758:765	Fourier transform-infrared analysis	731:765	Fourier transform-infrared analysis	731:765	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	6	9	theme	soluble	929:935	arg1	substance					937:945	a soluble substance	927:945	a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained	927:1085	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	3	10	theme	SPC-maltodextrin	533:548	arg1	SMC					562:564	SMC	562:564	SMC	562:564	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	3	10	theme	SPC-maltodextrin	533:548	arg1	conjugates					550:559	SPC-maltodextrin conjugates	533:559	SPC-maltodextrin conjugates (SMC)	533:565	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	4	11	theme	conjugated	586:595	arg1	substance					597:605	The conjugated substance	582:605	The conjugated substance	582:605	The conjugated substance was analyzed by means of different instrumental techniques.					
30342133	5	12	theme	transform-infrared	739:756	arg1	analysis					758:765	Fourier transform-infrared analysis	731:765	Fourier transform-infrared analysis	731:765	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	5	13	theme	covalent	671:678	arg1	attachment					680:689	The covalent attachment	667:689	The covalent attachment of maltodextrin to SPC	667:712	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	9	14	theme	quality	1496:1502	arg1	enhancement					1454:1464	the enhancement	1450:1464	the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1450:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	6	15	theme	SMC	1000:1002	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	6	15	theme	SMC	1000:1002	arg1	capability					986:995	The emulsifying capability	970:995	The emulsifying capability of SMC	970:1002	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	9	16	theme	food	1430:1433	arg1	ingredient					1435:1444	a food ingredient	1428:1444	a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1428:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	6	17	theme	poor	820:823	arg1	solubility					825:834	a poor solubility	818:834	a poor solubility that was affected by pH	818:858	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	3	18	theme	concentrate	520:530	arg1	properties					491:500	the functional properties	476:500	the functional properties of sesame protein concentrate	476:530	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	6	19	theme	smaller	1051:1057	arg1	sizes					1067:1071	smaller droplet sizes	1051:1071	smaller droplet sizes	1051:1071	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	8	20	theme	Structural	1200:1209	arg1	flexibility					1211:1221	Structural flexibility	1200:1221	Structural flexibility of the molecules	1200:1238	Structural flexibility of the molecules was increased, improving the functional characteristics of the protein.					
30342133	0	21	dep	by-products	102:112	arg1	Functional					115:124	Functional	115:124	Functional	115:124	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	0	21	dep	by-products	102:112	arg1	properties					147:156	physico-chemical properties	130:156	physico-chemical properties	130:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	8	22	theme	functional	1269:1278	arg1	characteristics					1280:1294	the functional characteristics	1265:1294	the functional characteristics of the protein	1265:1309	Structural flexibility of the molecules was increased, improving the functional characteristics of the protein.					
30342133	0	23	theme	new	2:4	arg1	conjugate					40:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	3	24	theme	protein	512:518	arg1	concentrate					520:530	sesame protein concentrate	505:530	sesame protein concentrate	505:530	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	7	25	theme	Circular	1088:1095	arg1	spectroscopy					1107:1118	Circular dichroism spectroscopy	1088:1118	Circular dichroism spectroscopy	1088:1118	Circular dichroism spectroscopy showed that the secondary structures were changed significantly by conjugation.					
30342133	6	26	from	substance	937:945	arg1	range					957:961	a wide range	950:961	a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained	950:1085	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	9	27	theme	value	1486:1490	arg1	enhancement					1454:1464	the enhancement	1450:1464	the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1450:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	28	theme	alternative	1371:1381	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	28	theme	alternative	1371:1381	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	7	29	theme	dichroism	1097:1105	arg1	spectroscopy					1107:1118	Circular dichroism spectroscopy	1088:1118	Circular dichroism spectroscopy	1088:1118	Circular dichroism spectroscopy showed that the secondary structures were changed significantly by conjugation.					
30342133	8	30	theme	protein	1303:1309	arg1	characteristics					1280:1294	the functional characteristics	1265:1294	the functional characteristics of the protein	1265:1309	Structural flexibility of the molecules was increased, improving the functional characteristics of the protein.					
30342133	2	31	theme	protein	361:367	arg1	SPC					382:384	SPC	382:384	SPC	382:384	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	2	31	theme	protein	361:367	arg1	protein					329:335	the protein	325:335	the protein	325:335	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	2	31	theme	protein	361:367	arg1	concentrate					369:379	sesame protein concentrate	354:379	sesame protein concentrate (SPC)	354:385	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	0	32	theme	protein‑polysaccharide	17:38	arg1	conjugate					40:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	1	33	theme	high	282:285	arg1	amount					287:292	a high amount	280:292	a high amount of proteins	280:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	33	theme	high	282:285	arg1	proteins					297:304	proteins	297:304	proteins	297:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	5	34	attach	attachment	680:689	arg1	SPC					710:712	SPC	710:712	SPC	710:712	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	5	34	attach	attachment	680:689	arg2	maltodextrin					694:705	maltodextrin	694:705	maltodextrin	694:705	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	2	35	theme	physicochemical	395:409	arg1	properties					426:435	its physicochemical and functional properties	391:435	its physicochemical and functional properties	391:435	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	0	36	theme	functional	6:15	arg1	conjugate					40:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate	0:48	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	6	37	theme	emulsifying	974:984	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	6	37	theme	emulsifying	974:984	arg1	capability					986:995	The emulsifying capability	970:995	The emulsifying capability of SMC	970:1002	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	0	38	theme	physico-chemical	130:145	arg1	properties					147:156	physico-chemical properties	130:156	physico-chemical properties	130:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	3	39	theme	sesame	505:510	arg1	concentrate					520:530	sesame protein concentrate	505:530	sesame protein concentrate	505:530	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	9	40	theme	protein	1399:1405	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	40	theme	protein	1399:1405	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	41	theme	oil	201:203	arg1	extraction					205:214	oil extraction	201:214	oil extraction	201:214	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	4	42	theme	instrumental	642:653	arg1	techniques					655:664	different instrumental techniques	632:664	different instrumental techniques	632:664	The conjugated substance was analyzed by means of different instrumental techniques.					
30342133	9	43	theme	food	1469:1472	arg1	value					1486:1490	food nutritional value	1469:1490	food nutritional value	1469:1490	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	44	theme	proteins	297:304	arg1	amount					287:292	a high amount	280:292	a high amount of proteins	280:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	44	theme	proteins	297:304	arg1	proteins					297:304	proteins	297:304	proteins	297:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	6	45	dep	pH.	966:968	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	6	45	dep	pH.	966:968	arg1	capability					986:995	The emulsifying capability	970:995	The emulsifying capability of SMC	970:1002	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	6	45	dep	pH.	966:968	arg1	obtained					1078:1085	obtained	1078:1085	were obtained	1073:1085	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	2	46	theme	functional	415:424	arg1	properties					426:435	its physicochemical and functional properties	391:435	its physicochemical and functional properties	391:435	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	1	47	from	processing	220:229	arg1	residue					174:180	Ardeh or Tahin residue	159:180	residue	174:180	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	47	from	processing	220:229	arg1	by-product					185:194	a by-product	183:194	a by-product from oil extraction and processing of dehulled, roasted and milled sesame	183:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	4	48	theme	different	632:640	arg1	techniques					655:664	different instrumental techniques	632:664	different instrumental techniques	632:664	The conjugated substance was analyzed by means of different instrumental techniques.					
30342133	0	49	theme	protein	59:65	arg1	concentrate					67:77	protein concentrate	59:77	protein concentrate from sesame processing by-products: Functional and physico-chemical properties	59:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	6	50	theme	pH.	966:968	arg1	range					957:961	a wide range	950:961	a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained	950:1085	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	9	51	theme	renewable	1383:1391	arg1	residue					1342:1348	Ardeh residue	1336:1348	Ardeh residue	1336:1348	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	51	theme	renewable	1383:1391	arg1	source					1407:1412	a potential alternative renewable plant protein source	1359:1412	a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin	1359:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	9	52	with	conjugation	1521:1531	arg1	maltodextrin					1538:1549	maltodextrin	1538:1549	maltodextrin	1538:1549	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	53	from	extraction	205:214	arg1	residue					174:180	Ardeh or Tahin residue	159:180	residue	174:180	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	53	from	extraction	205:214	arg1	by-product					185:194	a by-product	183:194	a by-product from oil extraction and processing of dehulled, roasted and milled sesame	183:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	3	54	theme	functional	480:489	arg1	properties					491:500	the functional properties	476:500	the functional properties of sesame protein concentrate	476:530	In order to improve the functional properties of sesame protein concentrate, SPC-maltodextrin conjugates (SMC) were prepared.					
30342133	6	55	theme	wide	952:955	arg1	range					957:961	a wide range	950:961	a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained	950:1085	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	5	56	theme	maltodextrin	694:705	arg1	attachment					680:689	The covalent attachment	667:689	The covalent attachment of maltodextrin to SPC	667:712	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	6	57	with	emulsions	1036:1044	arg1	sizes					1067:1071	smaller droplet sizes	1051:1071	smaller droplet sizes	1051:1071	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	1	58	contain	contains	271:278	arg1	by-product					185:194	a by-product	183:194	a by-product from oil extraction and processing of dehulled, roasted and milled sesame	183:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	58	contain	contains	271:278	arg1	residue					174:180	Ardeh or Tahin residue	159:180	residue	174:180	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	58	contain	contains	271:278	arg2	proteins					297:304	proteins	297:304	proteins	297:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	58	contain	contains	271:278	arg2	amount					287:292	a high amount	280:292	a high amount of proteins	280:304	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	59	theme	dehulled	234:241	arg1	sesame					263:268	dehulled, roasted and milled sesame	234:268	dehulled, roasted and milled sesame	234:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	60	theme	Ardeh	159:163	arg1	residue					174:180	Ardeh or Tahin residue	159:180	residue	174:180	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	60	theme	Ardeh	159:163	arg1	by-product					185:194	a by-product	183:194	a by-product from oil extraction and processing of dehulled, roasted and milled sesame	183:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	5	61	theme	SDS	771:773	arg1	electrophoresis					779:793	SDS gel electrophoresis	771:793	SDS gel electrophoresis	771:793	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	0	62	from	by-products	102:112	arg1	concentrate					67:77	protein concentrate	59:77	protein concentrate from sesame processing by-products: Functional and physico-chemical properties	59:156	A new functional protein‑polysaccharide conjugate based on protein concentrate from sesame processing by-products: Functional and physico-chemical properties.					
30342133	6	63	theme	droplet	1059:1065	arg1	sizes					1067:1071	smaller droplet sizes	1051:1071	smaller droplet sizes	1051:1071	The protein exhibited a poor solubility that was affected by pH, while conjugation significantly enhanced the solubility providing a soluble substance in a wide range of pH. The emulsifying capability of SMC was higher than that of SPC and emulsions with smaller droplet sizes were obtained.					
30342133	2	64	theme	sesame	354:359	arg1	SPC					382:384	SPC	382:384	SPC	382:384	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	2	64	theme	sesame	354:359	arg1	protein					329:335	the protein	325:335	the protein	325:335	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	2	64	theme	sesame	354:359	arg1	concentrate					369:379	sesame protein concentrate	354:379	sesame protein concentrate (SPC)	354:385	In this research, the protein was extracted as sesame protein concentrate (SPC) and its physicochemical and functional properties were investigated.					
30342133	9	65	theme	nutritional	1474:1484	arg1	value					1486:1490	food nutritional value	1469:1490	food nutritional value	1469:1490	The results showed that Ardeh residue could be a potential alternative renewable plant protein source to be used as a food ingredient for the enhancement of food nutritional value and quality especially after conjugation with maltodextrin.					
30342133	1	66	theme	roasted	244:250	arg1	sesame					263:268	dehulled, roasted and milled sesame	234:268	dehulled, roasted and milled sesame	234:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	5	67	theme	gel	775:777	arg1	electrophoresis					779:793	SDS gel electrophoresis	771:793	SDS gel electrophoresis	771:793	The covalent attachment of maltodextrin to SPC was confirmed by Fourier transform-infrared analysis and SDS gel electrophoresis.					
30342133	8	68	theme	molecules	1230:1238	arg1	flexibility					1211:1221	Structural flexibility	1200:1221	Structural flexibility of the molecules	1200:1238	Structural flexibility of the molecules was increased, improving the functional characteristics of the protein.					
30342133	1	69	theme	Tahin	168:172	arg1	residue					174:180	Ardeh or Tahin residue	159:180	residue	174:180	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
30342133	1	69	theme	Tahin	168:172	arg1	by-product					185:194	a by-product	183:194	a by-product from oil extraction and processing of dehulled, roasted and milled sesame	183:268	Ardeh or Tahin residue, a by-product from oil extraction and processing of dehulled, roasted and milled sesame, contains a high amount of proteins.					
31158418	2	0	attach	present	397:403	arg1	state					431:435	a nonsulfated, dormant state	408:435	a nonsulfated, dormant state in natural sources	408:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	0	attach	present	397:403	arg2	polysaccharides					369:383	highly bioactive sulfated polysaccharides	343:383	highly bioactive sulfated polysaccharides	343:383	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	4	1	theme	sulfation	877:885	arg1	compositions					852:863	varying saccharide compositions	833:863	varying saccharide compositions	833:863	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	1	theme	sulfation	877:885	arg1	masses					911:916	molecular masses	901:916	molecular masses (38-94,000 g/mol)	901:934	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	1	theme	sulfation	877:885	arg1	38-94,000 g/mol					919:933	38-94,000 g/mol	919:933	38-94,000 g/mol	919:933	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	1	theme	sulfation	877:885	arg1	degrees					866:872	degrees	866:872	degrees of sulfation (0.4-1.7)	866:895	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	1	2	theme	high	199:202	arg1	degree					204:209	a high degree	197:209	a high degree of diversity and attractive pharmacological properties	197:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	6	3	theme	branched	1185:1192	arg1	polysaccharide					1121:1134	The most active sulfated polysaccharide	1096:1134	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL)	1096:1155	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	3	theme	branched	1185:1192	arg1	glucan					1194:1199	a 94,000 g/mol branched glucan	1170:1199	a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions	1170:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	4	with	glucan	1194:1199	arg1	sulfates					1206:1213	sulfates	1206:1213	sulfates at C-6/C-3,6/C-2,3,6 positions	1206:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	0	5	theme	activities	141:150	arg1	Assessment					58:67	Assessment	58:67	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	5	6	dep	solubilized	994:1004	arg1	converted					1042:1050	converted	1042:1050	converted their hydroxyl functionality into sulfates	1042:1093	Typically, in a matter of minutes, this reagent not only solubilized polysaccharides but also chemically converted their hydroxyl functionality into sulfates.					
31158418	1	7	theme	pharmacological	239:253	arg1	properties					255:264	attractive pharmacological properties	228:264	attractive pharmacological properties	228:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	8	8	theme	huge	1436:1439	arg1	prospect					1441:1448	a huge prospect	1434:1448	a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles	1434:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	7	9	from	features	1398:1405	arg1	conclusion					1250:1259	conclusion	1250:1259	conclusion	1250:1259	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	2	10	theme	2.34-7.77 μg/mL	537:551	arg1	values					527:532	human cytomegalovirus EC50 values	500:532	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	10	theme	2.34-7.77 μg/mL	537:551	arg1	activity					490:497	their antiviral activity	474:497	their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity	474:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	4	11	theme	utilized	708:715	arg1	able					739:742	able	739:742	able	739:742	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	11	theme	utilized	708:715	arg1	reagent					727:733	the utilized oleum-DMF reagent	704:733	the utilized oleum-DMF reagent	704:733	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	7	12	theme	important	1266:1274	arg1	determinants					1276:1287	the important determinants	1262:1287	the important determinants of such compounds' antiviral activity	1262:1325	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	7	12	theme	important	1266:1274	arg1	degree					1336:1341	degree	1336:1341	(i) degree of sulfation	1332:1354	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	3	13	theme	entry	648:652	arg1	inhibition					628:637	the inhibition	624:637	the inhibition of virus entry	624:652	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	3	13	theme	entry	648:652	arg1	mode					666:669	the main mode	657:669	the main mode of antiviral action	657:689	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	1	14	theme	compound	173:180	arg1	libraries					182:190	compound libraries	173:190	compound libraries	173:190	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	8	15	theme	natural	1473:1479	arg1	libraries					1496:1504	natural source-derived libraries	1473:1504	natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles	1473:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	0	16	theme	efficiencies	93:104	arg1	Assessment					58:67	Assessment	58:67	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	2	17	theme	low	559:561	arg1	degree					563:568	a low degree	557:568	a low degree of cytotoxicity	557:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	8	18	theme	libraries	1496:1504	arg1	improvement					1458:1468	the improvement	1454:1468	the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles	1454:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	0	19	theme	structural	107:116	arg1	features					118:125	structural features	107:125	structural features	107:125	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	4	20	theme	natural	805:811	arg1	sources					813:819	various natural sources	797:819	various natural sources	797:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	1	21	with	provisioning	157:168	arg1	degree					204:209	a high degree	197:209	a high degree of diversity and attractive pharmacological properties	197:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	6	22	theme	active	1105:1110	arg1	EC50					1137:1140	EC50	1137:1140	EC50 of 2.62 μg/mL	1137:1154	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	22	theme	active	1105:1110	arg1	polysaccharide					1121:1134	The most active sulfated polysaccharide	1096:1134	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL)	1096:1155	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	22	theme	active	1105:1110	arg1	glucan					1194:1199	a 94,000 g/mol branched glucan	1170:1199	a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions	1170:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	2	23	theme	antiviral	480:488	arg1	values					527:532	human cytomegalovirus EC50 values	500:532	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	23	theme	antiviral	480:488	arg1	activity					490:497	their antiviral activity	474:497	their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity	474:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	8	24	theme	active	1528:1533	arg1	polysaccharides					1535:1549	biologically active polysaccharides	1515:1549	biologically active polysaccharides with diversified chemical profiles	1515:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	4	25	from	range	758:762	arg1	sources					813:819	various natural sources	797:819	various natural sources	797:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	7	26	from	mass	1372:1375	arg1	conclusion					1250:1259	conclusion	1250:1259	conclusion	1250:1259	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	4	27	theme	sulfated	767:774	arg1	polysaccharides					776:790	sulfated polysaccharides	767:790	sulfated polysaccharides from various natural sources	767:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	8	28	with	polysaccharides	1535:1549	arg1	profiles					1577:1584	diversified chemical profiles	1556:1584	diversified chemical profiles	1556:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	2	29	theme	cytomegalovirus	506:520	arg1	values					527:532	human cytomegalovirus EC50 values	500:532	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	29	theme	cytomegalovirus	506:520	arg1	activity					490:497	their antiviral activity	474:497	their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity	474:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	8	30	theme	chemical	1568:1575	arg1	profiles					1577:1584	diversified chemical profiles	1556:1584	diversified chemical profiles	1556:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	3	31	theme	antiviral	674:682	arg1	action					684:689	antiviral action	674:689	antiviral action	674:689	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	2	32	theme	natural	440:446	arg1	sources					448:454	natural sources	440:454	natural sources	440:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	0	33	theme	natural	41:47	arg1	sources					49:55	natural sources	41:55	natural sources	41:55	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	5	34	theme	hydroxyl	1058:1065	arg1	functionality					1067:1079	their hydroxyl functionality	1052:1079	their hydroxyl functionality	1052:1079	Typically, in a matter of minutes, this reagent not only solubilized polysaccharides but also chemically converted their hydroxyl functionality into sulfates.					
31158418	2	35	from	state	431:435	arg1	present					397:403	present	397:403	present	397:403	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	35	from	state	431:435	arg1	sources					448:454	natural sources	440:454	natural sources	440:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	36	theme	sulfated	360:367	arg1	polysaccharides					369:383	highly bioactive sulfated polysaccharides	343:383	highly bioactive sulfated polysaccharides	343:383	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	1	37	theme	properties	255:264	arg1	degree					204:209	a high degree	197:209	a high degree of diversity and attractive pharmacological properties	197:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	0	38	dep	polysaccharides	20:34	arg1	Assessment					58:67	Assessment	58:67	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	7	39	from	degree	1336:1341	arg1	conclusion					1250:1259	conclusion	1250:1259	conclusion	1250:1259	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	7	40	theme	antiviral	1308:1316	arg1	activity					1318:1325	such compounds' antiviral activity	1292:1325	such compounds' antiviral activity	1292:1325	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	2	41	from	degree	563:568	arg1	values					527:532	human cytomegalovirus EC50 values	500:532	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	41	from	degree	563:568	arg1	activity					490:497	their antiviral activity	474:497	their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity	474:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	4	42	theme	varying	833:839	arg1	compositions					852:863	varying saccharide compositions	833:863	varying saccharide compositions	833:863	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	2	43	theme	present	397:403	arg1	polysaccharides					369:383	highly bioactive sulfated polysaccharides	343:383	highly bioactive sulfated polysaccharides	343:383	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	1	44	theme	drug	288:291	arg1	development					293:303	drug development	288:303	drug development	288:303	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	4	45	theme	molecular	901:909	arg1	38-94,000 g/mol					919:933	38-94,000 g/mol	919:933	38-94,000 g/mol	919:933	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	45	theme	molecular	901:909	arg1	masses					911:916	molecular masses	901:916	molecular masses (38-94,000 g/mol)	901:934	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	7	46	dep	mass	1372:1375	arg1	ii					1358:1359	ii	1358:1359	ii	1358:1359	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	2	47	from	present	397:403	arg1	state					431:435	a nonsulfated, dormant state	408:435	a nonsulfated, dormant state in natural sources	408:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	6	48	theme	94,000 g/mol	1172:1183	arg1	polysaccharide					1121:1134	The most active sulfated polysaccharide	1096:1134	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL)	1096:1155	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	48	theme	94,000 g/mol	1172:1183	arg1	glucan					1194:1199	a 94,000 g/mol branched glucan	1170:1199	a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions	1170:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	1	49	theme	diversity	214:222	arg1	degree					204:209	a high degree	197:209	a high degree of diversity and attractive pharmacological properties	197:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	2	50	theme	cytotoxicity	573:584	arg1	degree					563:568	a low degree	557:568	a low degree of cytotoxicity	557:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	7	51	theme	structural	1387:1396	arg1	features					1398:1405	(iii) structural features	1381:1405	(iii) structural features	1381:1405	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	1	52	from	step	280:283	arg1	development					293:303	drug development	288:303	drug development	288:303	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	1	53	theme	attractive	228:237	arg1	properties					255:264	attractive pharmacological properties	228:264	attractive pharmacological properties	228:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	7	54	theme	molecular	1362:1370	arg1	mass					1372:1375	(ii) molecular mass	1357:1375	(ii) molecular mass	1357:1375	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	0	55	theme	extraction-sulfation	72:91	arg1	efficiencies					93:104	extraction-sulfation efficiencies	72:104	extraction-sulfation efficiencies	72:104	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	3	56	theme	virus	642:646	arg1	entry					648:652	virus entry	642:652	virus entry	642:652	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	8	57	theme	source-derived	1481:1494	arg1	libraries					1496:1504	natural source-derived libraries	1473:1504	natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles	1473:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	6	58	theme	C-6/C-3,6/C-2,3,6	1218:1234	arg1	positions					1236:1244	C-6/C-3,6/C-2,3,6 positions	1218:1244	C-6/C-3,6/C-2,3,6 positions	1218:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	59	from	positions	1236:1244	arg1	sulfates					1206:1213	sulfates	1206:1213	sulfates at C-6/C-3,6/C-2,3,6 positions	1206:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	4	60	from	sources	813:819	arg1	range					758:762	a range	756:762	a range of sulfated polysaccharides from various natural sources	756:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	60	from	sources	813:819	arg1	polysaccharides					776:790	sulfated polysaccharides	767:790	sulfated polysaccharides from various natural sources	767:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	1	61	theme	libraries	182:190	arg1	provisioning					157:168	The provisioning	153:168	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties	153:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	1	61	theme	libraries	182:190	arg1	step					280:283	a limiting step	269:283	a limiting step in drug development	269:303	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	2	62	theme	EC50	522:525	arg1	values					527:532	human cytomegalovirus EC50 values	500:532	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	62	theme	EC50	522:525	arg1	activity					490:497	their antiviral activity	474:497	their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity	474:584	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	4	63	theme	oleum-DMF	717:725	arg1	able					739:742	able	739:742	able	739:742	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	4	63	theme	oleum-DMF	717:725	arg1	reagent					727:733	the utilized oleum-DMF reagent	704:733	the utilized oleum-DMF reagent	704:733	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	0	64	theme	features	118:125	arg1	Assessment					58:67	Assessment	58:67	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	7	65	theme	sulfation	1346:1354	arg1	features					1398:1405	(iii) structural features	1381:1405	(iii) structural features	1381:1405	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	7	65	theme	sulfation	1346:1354	arg1	determinants					1276:1287	the important determinants	1262:1287	the important determinants of such compounds' antiviral activity	1262:1325	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	7	65	theme	sulfation	1346:1354	arg1	mass					1372:1375	(ii) molecular mass	1357:1375	(ii) molecular mass	1357:1375	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	7	65	theme	sulfation	1346:1354	arg1	degree					1336:1341	degree	1336:1341	(i) degree of sulfation	1332:1354	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	4	66	theme	polysaccharides	776:790	arg1	range					758:762	a range	756:762	a range of sulfated polysaccharides from various natural sources	756:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	6	67	theme	2.62 μg/mL	1145:1154	arg1	EC50					1137:1140	EC50	1137:1140	EC50 of 2.62 μg/mL	1137:1154	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	67	theme	2.62 μg/mL	1145:1154	arg1	polysaccharide					1121:1134	The most active sulfated polysaccharide	1096:1134	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL)	1096:1155	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	0	68	theme	antiviral	131:139	arg1	activities					141:150	antiviral activities	131:150	antiviral activities	131:150	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	6	69	theme	sulfated	1112:1119	arg1	EC50					1137:1140	EC50	1137:1140	EC50 of 2.62 μg/mL	1137:1154	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	69	theme	sulfated	1112:1119	arg1	polysaccharide					1121:1134	The most active sulfated polysaccharide	1096:1134	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL)	1096:1155	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	6	69	theme	sulfated	1112:1119	arg1	glucan					1194:1199	a 94,000 g/mol branched glucan	1170:1199	a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions	1170:1244	The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions.					
31158418	0	70	from	sources	49:55	arg1	polysaccharides					20:34	Chemically sulfated polysaccharides	0:34	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	4	71	theme	various	797:803	arg1	sources					813:819	various natural sources	797:819	various natural sources	797:819	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	0	72	theme	sulfated	11:18	arg1	polysaccharides					20:34	Chemically sulfated polysaccharides	0:34	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.	0:151	Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities.					
31158418	8	73	theme	diversified	1556:1566	arg1	profiles					1577:1584	diversified chemical profiles	1556:1584	diversified chemical profiles	1556:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	2	74	theme	nonsulfated	410:420	arg1	state					431:435	a nonsulfated, dormant state	408:435	a nonsulfated, dormant state in natural sources	408:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	5	75	theme	minutes	963:969	arg1	matter					953:958	a matter	951:958	a matter of minutes	951:969	Typically, in a matter of minutes, this reagent not only solubilized polysaccharides but also chemically converted their hydroxyl functionality into sulfates.					
31158418	7	76	dep	degree	1336:1341	arg1	i					1333:1333	i	1333:1333	i	1333:1333	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	2	77	theme	human	500:504	arg1	cytomegalovirus					506:520	human cytomegalovirus	500:520	human cytomegalovirus EC50 values of 2.34-7.77 μg/mL	500:551	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	3	78	theme	main	661:664	arg1	inhibition					628:637	the inhibition	624:637	the inhibition of virus entry	624:652	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	3	78	theme	main	661:664	arg1	mode					666:669	the main mode	657:669	the main mode of antiviral action	657:689	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	4	79	theme	saccharide	841:850	arg1	compositions					852:863	varying saccharide compositions	833:863	varying saccharide compositions	833:863	Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4-1.7) and molecular masses (38-94,000 g/mol).					
31158418	2	80	theme	polysaccharides	369:383	arg1	production					329:338	the production	325:338	the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources	325:454	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	8	81	link	source-derived	1481:1494	arg1	libraries					1496:1504	natural source-derived libraries	1473:1504	natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles	1473:1584	Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.					
31158418	3	82	theme	action	684:689	arg1	inhibition					628:637	the inhibition	624:637	the inhibition of virus entry	624:652	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	3	82	theme	action	684:689	arg1	mode					666:669	the main mode	657:669	the main mode of antiviral action	657:689	Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action.					
31158418	2	83	dep	nonsulfated	410:420	arg1	dormant					423:429	dormant	423:429	dormant	423:429	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	2	84	theme	bioactive	350:358	arg1	polysaccharides					369:383	highly bioactive sulfated polysaccharides	343:383	highly bioactive sulfated polysaccharides	343:383	This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34-7.77 μg/mL) at a low degree of cytotoxicity.					
31158418	7	85	theme	such	1292:1295	arg1	activity					1318:1325	such compounds' antiviral activity	1292:1325	such compounds' antiviral activity	1292:1325	In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features.					
31158418	1	86	theme	limiting	271:278	arg1	provisioning					157:168	The provisioning	153:168	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties	153:264	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
31158418	1	86	theme	limiting	271:278	arg1	step					280:283	a limiting step	269:283	a limiting step in drug development	269:303	The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development.					
29367749	0	0	theme	Stevia	91:96	arg1	A					121:121	Stevia component rebaudioside A	91:121	Stevia component rebaudioside A	91:121	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	5	1	theme	reaction	1132:1139	arg1	Development					1105:1115	3 h. Development	1100:1115	3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1100:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	1	2	from	the	179:181	arg1	sweeteners					246:255	high-potency natural sweeteners	225:255	high-potency natural sweeteners	225:255	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	1	2	from	the	179:181	arg1	glycosides					163:172	Steviol glycosides	155:172	Steviol glycosides from the leaves of the plant Stevia rebaudiana	155:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	1	3	theme	Steviol	155:161	arg1	sweeteners					246:255	high-potency natural sweeteners	225:255	high-potency natural sweeteners	225:255	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	1	3	theme	Steviol	155:161	arg1	glycosides					163:172	Steviol glycosides	155:172	Steviol glycosides from the leaves of the plant Stevia rebaudiana	155:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	7	4	theme	α-glucosylated	1510:1523	arg1	variants					1530:1537	α-glucosylated RebA variants	1510:1537	α-glucosylated RebA variants	1510:1537	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	7	5	theme	improved	1544:1551	arg1	properties					1577:1586	improved edulcorant/organoleptic properties	1544:1586	improved edulcorant/organoleptic properties	1544:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	3	6	theme	MALDI-TOF	522:530	arg1	spectrometry					537:548	MALDI-TOF mass spectrometry	522:548	MALDI-TOF mass spectrometry	522:548	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	4	7	theme	RebA	735:738	arg1	Docking					724:730	Docking	724:730	Docking of RebA in the active site of the enzyme	724:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	0	8	theme	rebaudioside	108:119	arg1	A					121:121	Stevia component rebaudioside A	91:121	Stevia component rebaudioside A	91:121	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	4	9	theme	enzyme	766:771	arg1	site					754:757	the active site	743:757	the active site of the enzyme	743:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	9	theme	enzyme	766:771	arg1	enzyme					766:771	the enzyme	762:771	the enzyme	762:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	6	10	theme	RebA	1299:1302	arg1	products					1304:1311	glucosylated RebA products	1286:1311	glucosylated RebA products	1286:1311	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	2	11	dep	particular	369:378	arg1	Q1140E-mutant					384:396	its Q1140E-mutant	380:396	particular its Q1140E-mutant	369:396	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	7	12	theme	Gtf180-ΔN-Q1140E	1417:1432	arg1	biocatalyst					1483:1493	an efficient biocatalyst	1470:1493	an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties	1470:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	7	12	theme	Gtf180-ΔN-Q1140E	1417:1432	arg1	enzyme					1455:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	0	13	theme	component	98:106	arg1	A					121:121	Stevia component rebaudioside A	91:121	Stevia component rebaudioside A	91:121	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	5	14	theme	3 h.	1100:1103	arg1	Development					1105:1115	3 h. Development	1100:1115	3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1100:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	6	15	theme	glucosylated	1286:1297	arg1	products					1304:1311	glucosylated RebA products	1286:1311	glucosylated RebA products	1286:1311	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	4	16	theme	active	747:752	arg1	site					754:757	the active site	743:757	the active site of the enzyme	743:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	16	theme	active	747:752	arg1	enzyme					766:771	the enzyme	762:771	the enzyme	762:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	7	17	theme	glucansucrase	1434:1446	arg1	biocatalyst					1483:1493	an efficient biocatalyst	1470:1493	an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties	1470:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	7	17	theme	glucansucrase	1434:1446	arg1	enzyme					1455:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	6	18	theme	superior	1372:1379	arg1	profile					1387:1393	a superior taste profile	1370:1393	a superior taste profile compared to RebA	1370:1410	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	3	19	theme	Structural	483:492	arg1	analysis					494:501	Structural analysis	483:501	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy	483:591	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	2	20	theme	rebaudioside	426:437	arg1	RebA					442:445	RebA	442:445	RebA	442:445	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	2	20	theme	rebaudioside	426:437	arg1	A					439:439	rebaudioside A	426:439	rebaudioside A (RebA)	426:446	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	7	21	theme	efficient	1473:1481	arg1	biocatalyst					1483:1493	an efficient biocatalyst	1470:1493	an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties	1470:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	7	21	theme	efficient	1473:1481	arg1	enzyme					1455:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	3	22	theme	methylation	551:561	arg1	analysis					563:570	methylation analysis	551:570	methylation analysis	551:570	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	7	23	theme	RebA	1525:1528	arg1	variants					1530:1537	α-glucosylated RebA variants	1510:1537	α-glucosylated RebA variants	1510:1537	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	3	24	theme	linkage	715:721	arg1	formation					692:700	the initial formation	680:700	the initial formation of an (α1→6) linkage	680:721	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	2	25	theme	wild-type	327:335	arg1	Gtf180-ΔN					351:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	5	26	theme	Response	861:868	arg1	methodology					878:888	Response surface methodology	861:888	Response surface methodology	861:888	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	0	27	theme	Glucansucrase	0:12	arg1	enzymes					23:29	Glucansucrase (mutant) enzymes	0:29	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180	0:60	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	1	28	theme	lingering	275:283	arg1	bitterness					285:294	a lingering bitterness	273:294	a lingering bitterness	273:294	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	6	29	theme	trained	1258:1264	arg1	panel					1266:1270	a trained panel	1256:1270	a trained panel	1256:1270	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	5	30	theme	RebA	964:967	arg1	α-glucosylation					945:959	the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation	914:959	the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA	914:967	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	31	theme	surface	870:876	arg1	methodology					878:888	Response surface methodology	861:888	Response surface methodology	861:888	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	7	32	theme	mutant	1448:1453	arg1	biocatalyst					1483:1493	an efficient biocatalyst	1470:1493	an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties	1470:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	7	32	theme	mutant	1448:1453	arg1	enzyme					1455:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme	1413:1460	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	4	33	theme	C-19	804:807	arg1	available					832:840	available	832:840	available	832:840	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	33	theme	C-19	804:807	arg1	moiety					822:827	only the steviol C-19 β-D-glucosyl moiety	787:827	only the steviol C-19 β-D-glucosyl moiety	787:827	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	3	34	theme	NMR	576:578	arg1	spectroscopy					580:591	NMR spectroscopy	576:591	NMR spectroscopy	576:591	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	5	35	theme	%	1039:1039	arg1	conversion					1023:1032	a RebA conversion	1016:1032	a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1016:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	0	36	theme	mutant	15:20	arg1	enzymes					23:29	Glucansucrase (mutant) enzymes	0:29	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180	0:60	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	3	37	theme	RebA	669:672	arg1	RebA					669:672	RebA	669:672	RebA	669:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	3	37	theme	RebA	669:672	arg1	residue					658:664	the Glc(β1→C-19 residue	642:664	the Glc(β1→C-19 residue of RebA	642:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	2	38	theme	Lactobacillus	301:313	arg1	Gtf180-ΔN					351:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	1	39	theme	plant	197:201	arg1	rebaudiana					210:219	the plant Stevia rebaudiana	193:219	the plant Stevia rebaudiana	193:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	5	40	theme	product	1210:1216	arg1	yield					1218:1222	the product yield	1206:1222	the product yield to 270 g/L	1206:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	3	41	theme	products	510:517	arg1	analysis					494:501	Structural analysis	483:501	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy	483:591	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	0	42	theme	superior	139:146	arg1	taste					148:152	a superior taste	137:152	a superior taste	137:152	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	5	43	theme	α-glucosylated	1069:1082	arg1	products					1084:1091	115 g/L α-glucosylated products	1061:1091	115 g/L α-glucosylated products	1061:1091	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	4	44	theme	steviol	796:802	arg1	available					832:840	available	832:840	available	832:840	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	44	theme	steviol	796:802	arg1	moiety					822:827	only the steviol C-19 β-D-glucosyl moiety	787:827	only the steviol C-19 β-D-glucosyl moiety	787:827	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	1	45	theme	Stevia	203:208	arg1	rebaudiana					210:219	the plant Stevia rebaudiana	193:219	the plant Stevia rebaudiana	193:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	5	46	theme	products	1084:1091	arg1	production					1047:1056	a production	1045:1056	a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1045:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	46	theme	products	1084:1091	arg1	products					1084:1091	115 g/L α-glucosylated products	1061:1091	115 g/L α-glucosylated products	1061:1091	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	46	theme	products	1084:1091	arg1	%					1039:1039	95%	1037:1039	95%	1037:1039	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	0	47	dep	Lactobacillus	36:48	arg1	reuteri					50:56	reuteri	50:56	reuteri	50:56	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	5	48	theme	synthesis	1181:1189	arg1	suppression					1157:1167	further suppression	1149:1167	further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1149:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	49	theme	115 g/L	1061:1067	arg1	products					1084:1091	115 g/L α-glucosylated products	1061:1091	115 g/L α-glucosylated products	1061:1091	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	0	50	from	180	58:60	arg1	enzymes					23:29	Glucansucrase (mutant) enzymes	0:29	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180	0:60	Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste.					
29367749	4	51	theme	β-D-glucosyl	809:820	arg1	available					832:840	available	832:840	available	832:840	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	51	theme	β-D-glucosyl	809:820	arg1	moiety					822:827	only the steviol C-19 β-D-glucosyl moiety	787:827	only the steviol C-19 β-D-glucosyl moiety	787:827	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	6	52	from	reduction	1332:1340	arg1	bitterness					1345:1354	bitterness	1345:1354	bitterness	1345:1354	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	2	53	theme	donor	466:470	arg1	substrate					472:480	donor substrate	466:480	donor substrate	466:480	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	5	54	theme	α-glucan	1172:1179	arg1	synthesis					1181:1189	α-glucan synthesis	1172:1189	α-glucan synthesis	1172:1189	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	3	55	theme	initial	684:690	arg1	formation					692:700	the initial formation	680:700	the initial formation of an (α1→6) linkage	680:721	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	1	56	dep	the	179:181	arg1	leaves					183:188	leaves	183:188	leaves	183:188	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	2	57	dep	Lactobacillus	301:313	arg1	reuteri					315:321	reuteri	315:321	reuteri	315:321	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	4	58	from	Docking	724:730	arg1	site					754:757	the active site	743:757	the active site of the enzyme	743:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	4	58	from	Docking	724:730	arg1	enzyme					766:771	the enzyme	762:771	the enzyme	762:771	Docking of RebA in the active site of the enzyme revealed that only the steviol C-19 β-D-glucosyl moiety is available for glucosylation.					
29367749	5	59	theme	RebA	1018:1021	arg1	conversion					1023:1032	a RebA conversion	1016:1032	a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1016:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	6	60	theme	significant	1320:1330	arg1	reduction					1332:1340	a significant reduction	1318:1340	a significant reduction in bitterness	1318:1354	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	5	61	theme	Gtf180-ΔN-Q1140E-catalyzed	918:943	arg1	α-glucosylation					945:959	the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation	914:959	the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA	914:967	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	62	theme	productive	992:1001	arg1	process					1003:1009	a highly productive process	983:1009	a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	983:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	5	63	theme	production	1047:1056	arg1	conversion					1023:1032	a RebA conversion	1016:1032	a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1016:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	3	64	theme	β1→C-19	650:656	arg1	RebA					669:672	RebA	669:672	RebA	669:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	3	64	theme	β1→C-19	650:656	arg1	residue					658:664	the Glc(β1→C-19 residue	642:664	the Glc(β1→C-19 residue of RebA	642:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	2	65	theme	glucansucrase	337:349	arg1	Gtf180-ΔN					351:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	180 wild-type glucansucrase Gtf180-ΔN	323:359	The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using sucrose as donor substrate.					
29367749	1	66	theme	high-potency	225:236	arg1	sweeteners					246:255	high-potency natural sweeteners	225:255	high-potency natural sweeteners	225:255	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	1	66	theme	high-potency	225:236	arg1	glycosides					163:172	Steviol glycosides	155:172	Steviol glycosides from the leaves of the plant Stevia rebaudiana	155:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	7	67	theme	edulcorant/organoleptic	1553:1575	arg1	properties					1577:1586	improved edulcorant/organoleptic properties	1544:1586	improved edulcorant/organoleptic properties	1544:1586	The Gtf180-ΔN-Q1140E glucansucrase mutant enzyme thus is an efficient biocatalyst for generating α-glucosylated RebA variants with improved edulcorant/organoleptic properties.					
29367749	5	68	theme	further	1149:1155	arg1	suppression					1157:1167	further suppression	1149:1167	further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1149:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	3	69	theme	mass	532:535	arg1	spectrometry					537:548	MALDI-TOF mass spectrometry	522:548	MALDI-TOF mass spectrometry	522:548	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	1	70	theme	natural	238:244	arg1	sweeteners					246:255	high-potency natural sweeteners	225:255	high-potency natural sweeteners	225:255	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	1	70	theme	natural	238:244	arg1	glycosides					163:172	Steviol glycosides	155:172	Steviol glycosides from the leaves of the plant Stevia rebaudiana	155:219	Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness.					
29367749	5	71	theme	fed-batch	1122:1130	arg1	reaction					1132:1139	a fed-batch reaction	1120:1139	a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1120:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	3	72	theme	Glc	646:648	arg1	RebA					669:672	RebA	669:672	RebA	669:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	3	72	theme	Glc	646:648	arg1	residue					658:664	the Glc(β1→C-19 residue	642:664	the Glc(β1→C-19 residue of RebA	642:672	Structural analysis of the products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that both enzymes exclusively glucosylate the Glc(β1→C-19 residue of RebA, with the initial formation of an (α1→6) linkage.					
29367749	6	73	theme	Sensory	1236:1242	arg1	analysis					1244:1251	Sensory analysis	1236:1251	Sensory analysis by a trained panel	1236:1270	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29367749	5	74	with	process	1003:1009	arg1	conversion					1023:1032	a RebA conversion	1016:1032	a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L	1016:1233	Response surface methodology was applied to optimize the Gtf180-ΔN-Q1140E-catalyzed α-glucosylation of RebA, resulting in a highly productive process with a RebA conversion of 95% and a production of 115 g/L α-glucosylated products within 3 h. Development of a fed-batch reaction allowed further suppression of α-glucan synthesis which improved the product yield to 270 g/L.					
29367749	6	75	theme	taste	1381:1385	arg1	profile					1387:1393	a superior taste profile	1370:1393	a superior taste profile compared to RebA	1370:1410	Sensory analysis by a trained panel revealed that glucosylated RebA products show a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.					
29376539	0	0	theme	NMR	93:95	arg1	data					106:109	their NMR spectral data	87:109	their NMR spectral data	87:109	Synthesis of oligosaccharides related to galactomannans from Aspergillus fumigatus and their NMR spectral data.					
29376539	3	1	theme	galactomannan	767:779	arg1	polysaccharides					789:803	galactomannan related polysaccharides	767:803	galactomannan related polysaccharides	767:803	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	3	2	theme	structural	744:753	arg1	analysis					755:762	further structural analysis	736:762	further structural analysis of galactomannan related polysaccharides	736:803	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	1	3	theme	antigenic	163:171	arg1	galactomannans					173:186	antigenic galactomannans	163:186	antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus	163:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	4	theme	NMR	871:873	arg1	spectra					875:881	the 13C NMR spectra	863:881	the 13C NMR spectra of polysaccharides	863:900	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	3	5	theme	chemical	642:649	arg1	shifts					651:656	13C NMR chemical shifts	634:656	13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	634:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	3	6	theme	spectra	875:881	arg1	calculation					848:858	the calculation	844:858	the calculation of the 13C NMR spectra of polysaccharides by additive schemes	844:920	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	7	theme	13C	537:539	arg1	signals					541:547	1H and 13C signals	530:547	1H and 13C signals	530:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	8	theme	benzoyl	346:352	arg1	migration					354:362	benzoyl migration	346:362	benzoyl migration	346:362	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	0	9	theme	spectral	97:104	arg1	data					106:109	their NMR spectral data	87:109	their NMR spectral data	87:109	Synthesis of oligosaccharides related to galactomannans from Aspergillus fumigatus and their NMR spectral data.					
29376539	2	10	theme	effects	596:602	arg1	determination					557:569	the determination	553:569	the determination of 13C NMR glycosylation effects	553:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	2	10	theme	effects	596:602	arg1	assignment					516:525	the full assignment	507:525	the full assignment of 1H and 13C signals	507:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	3	11	theme	13C	867:869	arg1	spectra					875:881	the 13C NMR spectra	863:881	the 13C NMR spectra of polysaccharides	863:900	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	12	theme	1H	530:531	arg1	signals					541:547	1H and 13C signals	530:547	1H and 13C signals	530:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	3	13	from	database	622:629	arg1	shifts					651:656	13C NMR chemical shifts	634:656	13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	634:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	14	theme	prepared	394:401	arg1	compounds					403:411	The prepared compounds	390:411	The prepared compounds along with some previously synthesized oligosaccharides	390:467	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	15	theme	key	367:369	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	15	theme	key	367:369	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	15	theme	key	367:369	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	16	theme	synthetic	371:379	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	16	theme	synthetic	371:379	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	16	theme	synthetic	371:379	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	17	theme	dangerous	195:203	arg1	pathogen					212:219	the dangerous fungal pathogen	191:219	the dangerous fungal pathogen Aspergillus fumigatus	191:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	18	theme	polysaccharides	789:803	arg1	analysis					755:762	further structural analysis	736:762	further structural analysis of galactomannan related polysaccharides	736:803	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	1	19	theme	fungal	205:210	arg1	pathogen					212:219	the dangerous fungal pathogen	191:219	the dangerous fungal pathogen Aspergillus fumigatus	191:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	2	20	theme	synthesized	440:450	arg1	oligosaccharides					452:467	some previously synthesized oligosaccharides	424:467	some previously synthesized oligosaccharides	424:467	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	21	theme	controlled	323:332	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	21	theme	controlled	323:332	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	21	theme	controlled	323:332	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	22	theme	pathogen	212:219	arg1	galactomannans					173:186	antigenic galactomannans	163:186	antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus	163:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	0	23	theme	oligosaccharides	13:28	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of oligosaccharides	0:28	Synthesis of oligosaccharides related to galactomannans from Aspergillus fumigatus and their NMR spectral data.					
29376539	1	24	theme	O	334:334	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	24	theme	O	334:334	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	24	theme	O	334:334	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	25	theme	Aspergillus	221:231	arg1	pathogen					212:219	the dangerous fungal pathogen	191:219	the dangerous fungal pathogen Aspergillus fumigatus	191:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	26	theme	related	781:787	arg1	polysaccharides					789:803	galactomannan related polysaccharides	767:803	galactomannan related polysaccharides	767:803	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	27	theme	NMR	485:487	arg1	spectroscopy					489:500	NMR spectroscopy	485:500	NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects	485:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	2	28	theme	signals	541:547	arg1	determination					557:569	the determination	553:569	the determination of 13C NMR glycosylation effects	553:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	2	28	theme	signals	541:547	arg1	assignment					516:525	the full assignment	507:525	the full assignment of 1H and 13C signals	507:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	3	29	theme	NMR	638:640	arg1	shifts					651:656	13C NMR chemical shifts	634:656	13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	634:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	3	30	theme	obtained	609:616	arg1	database					622:629	The obtained NMR database	605:629	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	605:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	31	theme	glycosylation	582:594	arg1	effects					596:602	13C NMR glycosylation effects	574:602	13C NMR glycosylation effects	574:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	0	32	from	fumigatus	73:81	arg1	galactomannans					41:54	galactomannans	41:54	galactomannans from Aspergillus fumigatus and their NMR spectral data	41:109	Synthesis of oligosaccharides related to galactomannans from Aspergillus fumigatus and their NMR spectral data.					
29376539	3	33	theme	further	736:742	arg1	analysis					755:762	further structural analysis	736:762	further structural analysis of galactomannan related polysaccharides	736:803	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	1	34	theme	→	339:339	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	34	theme	→	339:339	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	34	theme	→	339:339	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	2	35	theme	NMR	578:580	arg1	effects					596:602	13C NMR glycosylation effects	574:602	13C NMR glycosylation effects	574:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	3	36	theme	galactomannan	692:704	arg1	fragments					706:714	galactomannan fragments	692:714	galactomannan fragments	692:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	3	37	theme	non-destructive	810:824	arg1	approach					826:833	a non-destructive approach	808:833	a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes	808:920	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	38	theme	13C	574:576	arg1	NMR					578:580	13C NMR	574:580	13C NMR glycosylation effects	574:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	39	dep	rearrangement	305:317	arg1	migration					354:362	benzoyl migration	346:362	benzoyl migration	346:362	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	40	theme	model	129:133	arg1	oligosaccharides					135:150	model oligosaccharides	129:150	model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus	129:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	41	theme	additive	905:912	arg1	schemes					914:920	additive schemes	905:920	additive schemes	905:920	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	0	42	from	data	106:109	arg1	galactomannans					41:54	galactomannans	41:54	galactomannans from Aspergillus fumigatus and their NMR spectral data	41:109	Synthesis of oligosaccharides related to galactomannans from Aspergillus fumigatus and their NMR spectral data.					
29376539	3	43	theme	13C	634:636	arg1	shifts					651:656	13C NMR chemical shifts	634:656	13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	634:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	1	44	theme	oligosaccharides	135:150	arg1	synthesis					116:124	The synthesis	112:124	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus	112:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	45	theme	NMR	618:620	arg1	database					622:629	The obtained NMR database	605:629	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments	605:714	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	1	46	theme	related	152:158	arg1	oligosaccharides					135:150	model oligosaccharides	129:150	model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus	129:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	3	47	theme	polysaccharides	886:900	arg1	spectra					875:881	the 13C NMR spectra	863:881	the 13C NMR spectra of polysaccharides	863:900	The obtained NMR database on 13C NMR chemical shifts for oligosaccharides representing galactomannan fragments forms the basis for further structural analysis of galactomannan related polysaccharides by a non-destructive approach based on the calculation of the 13C NMR spectra of polysaccharides by additive schemes.					
29376539	2	48	theme	full	511:514	arg1	assignment					516:525	the full assignment	507:525	the full assignment of 1H and 13C signals	507:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	49	dep	Aspergillus	221:231	arg1	fumigatus					233:241	fumigatus	233:241	fumigatus	233:241	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	2	50	with	spectroscopy	489:500	arg1	determination					557:569	the determination	553:569	the determination of 13C NMR glycosylation effects	553:602	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	2	50	with	spectroscopy	489:500	arg1	assignment					516:525	the full assignment	507:525	the full assignment of 1H and 13C signals	507:547	The prepared compounds along with some previously synthesized oligosaccharides were studied by NMR spectroscopy with the full assignment of 1H and 13C signals and the determination of 13C NMR glycosylation effects.					
29376539	1	51	theme	pyranoside-into-furanoside	272:297	arg1	methods					381:387	key synthetic methods	367:387	key synthetic methods	367:387	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	51	theme	pyranoside-into-furanoside	272:297	arg1	O					341:341	controlled O(5) → O(6)	323:344	controlled O(5) → O(6)	323:344	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
29376539	1	51	theme	pyranoside-into-furanoside	272:297	arg1	rearrangement					305:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	pyranoside-into-furanoside (PIF) rearrangement	272:317	The synthesis of model oligosaccharides related to antigenic galactomannans of the dangerous fungal pathogen Aspergillus fumigatus has been performed employing pyranoside-into-furanoside (PIF) rearrangement and controlled O(5) → O(6) benzoyl migration as key synthetic methods.					
30169672	3	0	theme	=	735:735	arg1	tissues					724:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues	672:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	672:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	0	theme	=	735:735	arg1	n					733:733	n = 3	733:737	n = 3	733:737	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	6	1	theme	ovarian	1436:1442	arg1	tissues					1444:1450	diabetic ovarian tissues	1427:1450	diabetic ovarian tissues	1427:1450	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	7	2	from	increase	1501:1508	arg1	cytokines					1542:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	2	3	theme	protein	331:337	arg1	changes					353:359	protein glycosylation changes	331:359	protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation	331:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	6	4	attach	present	1274:1280	arg1	amount					1228:1233	a very low amount	1217:1233	a very low amount	1217:1233	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	6	4	attach	present	1274:1280	arg2	glycans					1245:1251	O-GalNAc glycans	1236:1251	O-GalNAc glycans of mice ovaries	1236:1267	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	6	5	theme	mice	1256:1259	arg1	ovaries					1261:1267	mice ovaries	1256:1267	mice ovaries	1256:1267	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	5	6	theme	ovarian	1088:1094	arg1	tissue					1096:1101	diabetic ovarian tissue	1079:1101	diabetic ovarian tissue of the same mouse	1079:1119	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	6	7	with	present	1274:1280	arg1	increase					1338:1345	a relative increase	1327:1345	a relative increase in the NeuGc:NeuAc ratio	1327:1370	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	8	from	proteins	944:951	arg1	decrease					785:792	a relative decrease	774:792	a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures	774:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	8	from	proteins	944:951	arg1	increase					899:906	an increase	896:906	an increase in oligomannose structures on their proteins	896:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	1	9	theme	follicle	266:273	arg1	development					275:285	ovarian follicle development	258:285	ovarian follicle development	258:285	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	7	10	theme	protein	1599:1605	arg1	glycosylation					1607:1619	protein glycosylation	1599:1619	protein glycosylation	1599:1619	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	4	11	theme	oligomannose	911:922	arg1	structures					924:933	oligomannose structures	911:933	oligomannose structures on their proteins	911:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	6	12	theme	O-GalNAc	1236:1243	arg1	glycans					1245:1251	O-GalNAc glycans	1236:1251	O-GalNAc glycans of mice ovaries	1236:1267	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	13	theme	mouse	750:754	arg1	ovaries					756:762	Diabetic mouse ovaries	741:762	Diabetic mouse ovaries	741:762	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	14	theme	O-glycans	643:651	arg1	identification					605:618	the identification	601:618	the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	601:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	1	15	theme	proteins	142:149	arg1	modification					126:137	Post-translational modification	107:137	Post-translational modification of proteins namely glycosylation	107:170	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	6	16	theme	diabetic	1427:1434	arg1	tissues					1444:1450	diabetic ovarian tissues	1427:1450	diabetic ovarian tissues	1427:1450	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	6	17	theme	NeuAc	1360:1364	arg1	ratio					1366:1370	the NeuGc:NeuAc ratio	1350:1370	the NeuGc:NeuAc ratio	1350:1370	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	18	from	increase	899:906	arg1	structures					924:933	oligomannose structures	911:933	oligomannose structures on their proteins	911:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	18	from	increase	899:906	arg1	proteins					944:951	their proteins	938:951	their proteins	938:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	18	from	increase	899:906	arg1	sialylation					797:807	sialylation	797:807	sialylation	797:807	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	1	19	theme	cellular	183:190	arg1	behavior					192:199	cellular behavior	183:199	cellular behavior	183:199	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	5	20	from	evident	1068:1074	arg1	tissue					1096:1101	diabetic ovarian tissue	1079:1101	diabetic ovarian tissue of the same mouse	1079:1119	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	4	21	theme	nondiabetic	968:978	arg1	ovaries					986:992	nondiabetic mouse ovaries	968:992	nondiabetic mouse ovaries	968:992	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	22	from	identification	605:618	arg1	proteins					660:667	the proteins	656:667	the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	656:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	5	23	theme	same	1110:1113	arg1	mouse					1115:1119	the same mouse	1106:1119	the same mouse	1106:1119	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	0	24	gly	glycosylation	77:89	arg1	ovary					100:104	mouse ovary	94:104	mouse ovary	94:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	7	25	theme	inflammatory	1529:1540	arg1	cytokines					1542:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	6	26	theme	relative	1329:1336	arg1	increase					1338:1345	a relative increase	1327:1345	a relative increase in the NeuGc:NeuAc ratio	1327:1370	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	5	27	from	effect	1151:1156	arg1	glycosylation					1190:1202	protein glycosylation	1182:1202	protein glycosylation	1182:1202	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	4	28	theme	relative	776:783	arg1	decrease					785:792	a relative decrease	774:792	a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures	774:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	2	29	theme	diabetes	447:454	arg1	influence					434:442	the well-known influence	419:442	the well-known influence of diabetes on the outcome of successful embryo implantation	419:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	3	30	from	O-glycans	643:651	arg1	proteins					660:667	the proteins	656:667	the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	656:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	2	31	theme	embryo	485:490	arg1	implantation					492:503	successful embryo implantation	474:503	successful embryo implantation	474:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	7	32	theme	TNF-α	1513:1517	arg1	cytokines					1542:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	3	33	theme	mouse	710:714	arg1	tissues					724:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues	672:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	672:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	33	theme	mouse	710:714	arg1	STZ					696:698	STZ	696:698	STZ	696:698	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	33	theme	mouse	710:714	arg1	n					733:733	n = 3	733:737	n = 3	733:737	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	0	34	theme	hyperglycemia	37:49	arg1	effect					4:9	The effect	0:9	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary	0:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	5	35	theme	diabetes	1161:1168	arg1	mellitus					1170:1177	diabetes mellitus	1161:1177	diabetes mellitus	1161:1177	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	0	36	theme	protein	69:75	arg1	glycosylation					77:89	N-and O-linked protein glycosylation	54:89	N-and O-linked protein glycosylation in mouse ovary	54:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	4	37	theme	N-linked	857:864	arg1	structures					873:882	branched N-linked glycan structures	848:882	branched N-linked glycan structures	848:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	38	theme	chromatography-ESI	535:552	arg1	spectrometry					559:570	PGC chromatography-ESI mass spectrometry	531:570	PGC chromatography-ESI mass spectrometry	531:570	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	0	39	theme	N-and	54:58	arg1	glycosylation					77:89	N-and O-linked protein glycosylation	54:89	N-and O-linked protein glycosylation in mouse ovary	54:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	3	40	from	use	524:526	arg1	mode					588:591	negative ion mode	575:591	negative ion mode	575:591	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	41	theme	tissues	724:730	arg1	proteins					660:667	the proteins	656:667	the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	656:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	4	42	theme	lesser	833:838	arg1	extent					840:845	a lesser extent	831:845	a lesser extent	831:845	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	43	theme	negative	575:582	arg1	mode					588:591	negative ion mode	575:591	negative ion mode	575:591	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	5	44	theme	diabetic	1032:1039	arg1	tissue					1047:1052	the diabetic liver tissue	1028:1052	the diabetic liver tissue	1028:1052	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	3	45	theme	spectrometry	559:570	arg1	use					524:526	the use	520:526	the use of PGC chromatography-ESI mass spectrometry in negative ion mode	520:591	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	7	46	theme	STZ-treated	1453:1463	arg1	mice					1465:1468	STZ-treated mice	1453:1468	STZ-treated mice	1453:1468	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	6	47	theme	core	1285:1288	arg1	type					1290:1293	core type 1	1285:1295	core type 1	1285:1295	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	2	48	theme	glycosylation	339:351	arg1	changes					353:359	protein glycosylation changes	331:359	protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation	331:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	5	49	from	Changes	995:1001	arg1	N-glycans					1006:1014	N-glycans	1006:1014	N-glycans	1006:1014	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	2	50	theme	diabetes	374:381	arg1	mellitus					383:390	diabetes mellitus	374:390	diabetes mellitus	374:390	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	1	51	theme	structural	202:211	arg1	properties					213:222	structural properties	202:222	structural properties	202:222	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	6	52	dep	type	1290:1293	arg1	glycans					1313:1319	glycans	1313:1319	glycans	1313:1319	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	53	theme	glycan	866:871	arg1	structures					873:882	branched N-linked glycan structures	848:882	branched N-linked glycan structures	848:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	0	54	link	O-linked	60:67	arg1	glycosylation					77:89	N-and O-linked protein glycosylation	54:89	N-and O-linked protein glycosylation in mouse ovary	54:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	6	55	theme	NeuGc	1354:1358	arg1	ratio					1366:1370	the NeuGc:NeuAc ratio	1350:1370	the NeuGc:NeuAc ratio	1350:1370	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	6	56	theme	ovaries	1261:1267	arg1	glycans					1245:1251	O-GalNAc glycans	1236:1251	O-GalNAc glycans of mice ovaries	1236:1267	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	3	57	theme	N-glycans	627:635	arg1	identification					605:618	the identification	601:618	the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	601:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	2	58	from	influence	434:442	arg1	outcome					463:469	the outcome	459:469	the outcome of successful embryo implantation	459:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	2	59	theme	ovarian	395:401	arg1	tissues					403:409	ovarian tissues	395:409	ovarian tissues	395:409	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	1	60	theme	ovarian	258:264	arg1	development					275:285	ovarian follicle development	258:285	ovarian follicle development	258:285	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	0	61	from	glycosylation	77:89	arg1	ovary					100:104	mouse ovary	94:104	mouse ovary	94:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	4	62	theme	Diabetic	741:748	arg1	ovaries					756:762	Diabetic mouse ovaries	741:762	Diabetic mouse ovaries	741:762	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	6	63	theme	core	1301:1304	arg1	type					1306:1309	core type 2	1301:1311	core type 2	1301:1311	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	64	from	structures	924:933	arg1	proteins					944:951	their proteins	938:951	their proteins	938:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	65	theme	Streptozotocin-induced	672:693	arg1	tissues					724:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues	672:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	672:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	65	theme	Streptozotocin-induced	672:693	arg1	STZ					696:698	STZ	696:698	STZ	696:698	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	65	theme	Streptozotocin-induced	672:693	arg1	n					733:733	n = 3	733:737	n = 3	733:737	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	6	66	theme	low	1224:1226	arg1	amount					1228:1233	a very low amount	1217:1233	a very low amount	1217:1233	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	3	67	from	N-glycans	627:635	arg1	proteins					660:667	the proteins	656:667	the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	656:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	5	68	theme	diabetic	1079:1086	arg1	tissue					1096:1101	diabetic ovarian tissue	1079:1101	diabetic ovarian tissue of the same mouse	1079:1119	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	0	69	theme	mouse	94:98	arg1	ovary					100:104	mouse ovary	94:104	mouse ovary	94:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	5	70	theme	organ-specific	1136:1149	arg1	effect					1151:1156	an organ-specific effect	1133:1156	an organ-specific effect of diabetes mellitus on protein glycosylation	1133:1202	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	6	71	from	increase	1338:1345	arg1	ratio					1366:1370	the NeuGc:NeuAc ratio	1350:1370	the NeuGc:NeuAc ratio	1350:1370	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	0	72	from	effect	4:9	arg1	glycosylation					77:89	N-and O-linked protein glycosylation	54:89	N-and O-linked protein glycosylation in mouse ovary	54:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	6	73	from	amount	1228:1233	arg1	present					1274:1280	present	1274:1280	present	1274:1280	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	7	74	theme	IL1-B	1523:1527	arg1	cytokines					1542:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	TNF-α and IL1-B inflammatory cytokines	1513:1550	STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.					
30169672	3	75	from	proteins	660:667	arg1	identification					605:618	the identification	601:618	the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	601:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	5	76	theme	mouse	1115:1119	arg1	tissue					1096:1101	diabetic ovarian tissue	1079:1101	diabetic ovarian tissue of the same mouse	1079:1119	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	6	77	located	present	1274:1280	arg2	glycans					1245:1251	O-GalNAc glycans	1236:1251	O-GalNAc glycans of mice ovaries	1236:1267	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	6	77	located	present	1274:1280	arg1	amount					1228:1233	a very low amount	1217:1233	a very low amount	1217:1233	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	4	78	link	N-linked	857:864	arg1	structures					873:882	branched N-linked glycan structures	848:882	branched N-linked glycan structures	848:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	79	theme	mouse	980:984	arg1	ovaries					986:992	nondiabetic mouse ovaries	968:992	nondiabetic mouse ovaries	968:992	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	1	80	dep	proteins	142:149	arg1	glycosylation					158:170	glycosylation	158:170	proteins namely glycosylation	142:170	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	4	81	from	decrease	785:792	arg1	structures					924:933	oligomannose structures	911:933	oligomannose structures on their proteins	911:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	81	from	decrease	785:792	arg1	proteins					944:951	their proteins	938:951	their proteins	938:951	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	4	81	from	decrease	785:792	arg1	sialylation					797:807	sialylation	797:807	sialylation	797:807	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	0	82	theme	streptozotocin-induced	14:35	arg1	hyperglycemia					37:49	streptozotocin-induced hyperglycemia	14:49	streptozotocin-induced hyperglycemia	14:49	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	1	83	theme	Post-translational	107:124	arg1	modification					126:137	Post-translational modification	107:137	Post-translational modification of proteins namely glycosylation	107:170	Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia.					
30169672	2	84	theme	implantation	492:503	arg1	outcome					463:469	the outcome	459:469	the outcome of successful embryo implantation	459:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	3	85	theme	diabetic	701:708	arg1	tissues					724:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues	672:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	672:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	85	theme	diabetic	701:708	arg1	STZ					696:698	STZ	696:698	STZ	696:698	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	85	theme	diabetic	701:708	arg1	n					733:733	n = 3	733:737	n = 3	733:737	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	2	86	theme	successful	474:483	arg1	implantation					492:503	successful embryo implantation	474:503	successful embryo implantation	474:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	6	87	theme	significant	1384:1394	arg1	difference					1396:1405	the most significant difference	1375:1405	the most significant difference between control and diabetic ovarian tissues	1375:1450	Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues.					
30169672	5	88	theme	liver	1041:1045	arg1	tissue					1047:1052	the diabetic liver tissue	1028:1052	the diabetic liver tissue	1028:1052	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	5	89	from	tissue	1096:1101	arg1	evident					1068:1074	evident	1068:1074	evident	1068:1074	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	0	90	theme	O-linked	60:67	arg1	glycosylation					77:89	N-and O-linked protein glycosylation	54:89	N-and O-linked protein glycosylation in mouse ovary	54:104	The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.					
30169672	5	91	theme	mellitus	1170:1177	arg1	effect					1151:1156	an organ-specific effect	1133:1156	an organ-specific effect of diabetes mellitus on protein glycosylation	1133:1202	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	2	92	theme	well-known	423:432	arg1	influence					434:442	the well-known influence	419:442	the well-known influence of diabetes on the outcome of successful embryo implantation	419:503	However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation.					
30169672	4	93	theme	branched	848:855	arg1	structures					873:882	branched N-linked glycan structures	848:882	branched N-linked glycan structures	848:882	Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries.					
30169672	3	94	theme	mass	554:557	arg1	spectrometry					559:570	PGC chromatography-ESI mass spectrometry	531:570	PGC chromatography-ESI mass spectrometry	531:570	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	5	95	theme	protein	1182:1188	arg1	glycosylation					1190:1202	protein glycosylation	1182:1202	protein glycosylation	1182:1202	Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation.					
30169672	3	96	theme	ovarian	716:722	arg1	tissues					724:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues	672:730	Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3)	672:738	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	96	theme	ovarian	716:722	arg1	STZ					696:698	STZ	696:698	STZ	696:698	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	96	theme	ovarian	716:722	arg1	n					733:733	n = 3	733:737	n = 3	733:737	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	97	theme	PGC	531:533	arg1	spectrometry					559:570	PGC chromatography-ESI mass spectrometry	531:570	PGC chromatography-ESI mass spectrometry	531:570	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
30169672	3	98	theme	ion	584:586	arg1	mode					588:591	negative ion mode	575:591	negative ion mode	575:591	In our study, the use of PGC chromatography-ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3).					
29638186	6	0	theme	H.	1069:1070	arg1	binding					1077:1083	H. suis binding	1069:1083	H. suis binding to mucins	1069:1093	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	5	1	located	present	1036:1042	arg2	14					1028:1029	14	1028:1029	14	1028:1029	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	1	located	present	1036:1042	arg1	samples					1060:1066	all examined samples	1047:1066	all examined samples	1047:1066	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	2	from	present	1036:1042	arg1	samples					1060:1066	all examined samples	1047:1066	all examined samples	1047:1066	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	6	3	theme	sialic	1139:1144	arg1	acid					1146:1149	sialic acid	1139:1149	sialic acid	1139:1149	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	6	3	theme	sialic	1139:1144	arg1	glycans					1111:1117	glycans	1111:1117	glycans containing sulfate	1111:1136	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	0	4	theme	Helicobacter	0:11	arg1	binding					18:24	Helicobacter suis binding	0:24	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids	0:93	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	1	5	theme	pigs	165:168	arg1	stomach					149:155	the stomach	145:155	the stomach of most pigs	145:168	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	7	6	theme	neutral	1357:1363	arg1	pH					1365:1366	neutral pH	1357:1366	neutral pH	1357:1366	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	5	7	attach	present	1036:1042	arg2	14					1028:1029	14	1028:1029	14	1028:1029	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	7	attach	present	1036:1042	arg1	samples					1060:1066	all examined samples	1047:1066	all examined samples	1047:1066	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	8	from	samples	1060:1066	arg1	present					1036:1042	present	1036:1042	present	1036:1042	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	7	9	from	pH	1365:1366	arg1	adhesion					1304:1311	adhesion	1304:1311	adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH	1304:1366	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	3	10	theme	H.	506:507	arg1	level					497:501	the level	493:501	the level of H. pylori and H. suis binding to human and pig gastric mucins	493:566	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	1	11	theme	non-Helicobacter	196:211	arg1	species					233:239	the most prevalent non-Helicobacter pylori Helicobacter species	177:239	the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach	177:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	4	12	theme	H.	654:655	arg1	optimum					643:649	The binding optimum	631:649	The binding optimum of H. pylori	631:662	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	9	13	dep	structures	1652:1661	arg1	H.					1663:1664	H.	1663:1664	H.	1663:1664	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	9	14	dep	H.	1663:1664	arg1	suis					1666:1669	H. suis	1663:1669	the glycan structures H. suis	1641:1669	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	8	15	contain	has	1513:1515	arg2	modes					1529:1533	two binding modes	1517:1533	two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures	1517:1620	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	15	contain	has	1513:1515	arg2	one					1585:1587	one	1585:1587	one	1585:1587	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	15	contain	has	1513:1515	arg1	H.					1505:1506	H.	1505:1506	H.	1505:1506	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	15	contain	has	1513:1515	arg2	one					1536:1538	one	1536:1538	one	1536:1538	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	3	16	theme	gastric	553:559	arg1	mucins					561:566	human and pig gastric mucins	539:566	human and pig gastric mucins	539:566	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	4	17	contain	has	705:707	arg2	optimum					722:728	an acidic pH optimum	709:728	an acidic pH optimum	709:728	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	4	17	contain	has	705:707	arg1	that					689:692	that	689:692	that	689:692	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	1	18	theme	human	254:258	arg1	stomach					260:266	the human stomach	250:266	the human stomach	250:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	3	19	dep	H.	506:507	arg1	pylori					509:514	H. pylori	506:514	H. pylori	506:514	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	5	20	theme	O-glycans	853:861	arg1	analysis					835:842	Mass spectrometric analysis	816:842	Mass spectrometric analysis of mucin O-glycans from the porcine mucin	816:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	9	21	theme	glycan	1645:1650	arg1	structures					1652:1661	the glycan structures H. suis	1641:1669	the glycan structures H. suis	1641:1669	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	5	22	theme	oligosaccharide	984:998	arg1	structures					1000:1009	109 oligosaccharide structures	980:1009	109 oligosaccharide structures identified	980:1020	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	0	23	from	carbohydrates	29:41	arg1	mucins					72:77	human and porcine gastric mucins	46:77	mucins	72:77	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	0	23	from	carbohydrates	29:41	arg1	glycolipids					83:93	glycolipids	83:93	glycolipids	83:93	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	0	24	theme	gastric	64:70	arg1	mucins					72:77	human and porcine gastric mucins	46:77	mucins	72:77	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	4	25	theme	neutral	670:676	arg1	pH					678:679	neutral pH	670:679	neutral pH	670:679	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	2	26	theme	ulcer	356:360	arg1	disease					362:368	peptic ulcer disease	349:368	peptic ulcer disease	349:368	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	4	27	dep	H.	751:752	arg1	pylori					754:759	H. pylori	751:759	H. pylori	751:759	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	5	28	theme	examined	1051:1058	arg1	samples					1060:1066	all examined samples	1047:1066	all examined samples	1047:1066	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	29	theme	spectrometric	821:833	arg1	analysis					835:842	Mass spectrometric analysis	816:842	Mass spectrometric analysis of mucin O-glycans from the porcine mucin	816:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	7	30	theme	pig	1208:1210	arg1	stomach					1212:1218	pig stomach	1208:1218	pig stomach	1208:1218	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	7	31	theme	present	1197:1203	arg1	glycolipids					1185:1195	the glycolipids	1181:1195	the glycolipids present in pig stomach	1181:1218	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	6	32	contain	containing	1119:1128	arg2	sulfate					1130:1136	sulfate	1130:1136	sulfate	1130:1136	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	6	32	contain	containing	1119:1128	arg1	galactose					1164:1172	terminal galactose	1155:1172	terminal galactose	1155:1172	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	6	32	contain	containing	1119:1128	arg1	acid					1146:1149	sialic acid	1139:1149	sialic acid	1139:1149	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	6	32	contain	containing	1119:1128	arg1	glycans					1111:1117	glycans	1111:1117	glycans containing sulfate	1111:1136	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	2	33	theme	lymphoma	379:386	arg1	development					315:325	the development	311:325	the development of chronic gastritis, peptic ulcer disease and MALT lymphoma	311:386	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	3	34	dep	H.	520:521	arg1	suis					523:526	H. suis	520:526	H. suis	520:526	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	0	35	theme	porcine	56:62	arg1	mucins					72:77	human and porcine gastric mucins	46:77	mucins	72:77	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	0	36	theme	human	46:50	arg1	mucins					72:77	human and porcine gastric mucins	46:77	mucins	72:77	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	1	37	theme	pylori	213:218	arg1	species					233:239	the most prevalent non-Helicobacter pylori Helicobacter species	177:239	the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach	177:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	4	38	dep	H.	697:698	arg1	suis					700:703	H. suis	697:703	H. suis	697:703	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	9	39	theme	strategies	1731:1740	arg1	development					1704:1714	development	1704:1714	development of therapeutic strategies alternative to antibiotics	1704:1767	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	4	40	theme	pH	719:720	arg1	optimum					722:728	an acidic pH optimum	709:728	an acidic pH optimum	709:728	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	8	41	theme	binding	1521:1527	arg1	one					1585:1587	one	1585:1587	one	1585:1587	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	41	theme	binding	1521:1527	arg1	modes					1529:1533	two binding modes	1517:1533	two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures	1517:1620	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	41	theme	binding	1521:1527	arg1	one					1536:1538	one	1536:1538	one	1536:1538	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	7	42	theme	other	1388:1392	arg1	glycoconjugates					1394:1408	other glycoconjugates	1388:1408	other glycoconjugates	1388:1408	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	0	43	dep	Helicobacter	0:11	arg1	suis					13:16	suis	13:16	suis	13:16	Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes.					
29638186	7	44	from	stomach	1212:1218	arg1	present					1197:1203	present	1197:1203	present	1197:1203	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	2	45	theme	chronic	330:336	arg1	gastritis					338:346	chronic gastritis	330:346	chronic gastritis	330:346	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	6	46	theme	terminal	1155:1162	arg1	glycans					1111:1117	glycans	1111:1117	glycans containing sulfate	1111:1136	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	6	46	theme	terminal	1155:1162	arg1	galactose					1164:1172	terminal galactose	1155:1172	terminal galactose	1155:1172	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	2	47	theme	human	276:280	arg1	host					282:285	the human host	272:285	the human host	272:285	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	3	48	dep	dependent	608:616	arg1	species					600:606	species	600:606	species	600:606	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	1	49	theme	most	160:163	arg1	pigs					165:168	most pigs	160:168	most pigs	160:168	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	5	50	from	mucin	880:884	arg1	analysis					835:842	Mass spectrometric analysis	816:842	Mass spectrometric analysis of mucin O-glycans from the porcine mucin	816:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	50	from	mucin	880:884	arg1	O-glycans					853:861	mucin O-glycans	847:861	mucin O-glycans from the porcine mucin	847:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	51	from	variation	909:917	arg1	binding					922:928	binding	922:928	binding	922:928	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	1	52	theme	prevalent	186:194	arg1	species					233:239	the most prevalent non-Helicobacter pylori Helicobacter species	177:239	the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach	177:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	3	53	theme	H.	520:521	arg1	level					497:501	the level	493:501	the level of H. pylori and H. suis binding to human and pig gastric mucins	493:566	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	8	54	theme	charged	1603:1609	arg1	structures					1611:1620	negatively charged structures	1592:1620	negatively charged structures	1592:1620	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	3	55	theme	pig	549:551	arg1	mucins					561:566	human and pig gastric mucins	539:566	human and pig gastric mucins	539:566	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	8	56	dep	modes	1529:1533	arg1	one					1585:1587	one	1585:1587	one	1585:1587	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	56	dep	modes	1529:1533	arg1	modes					1529:1533	two binding modes	1517:1533	two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures	1517:1620	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	8	56	dep	modes	1529:1533	arg1	one					1536:1538	one	1536:1538	one	1536:1538	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	2	57	dep	H.	288:289	arg1	suis					291:294	H. suis	288:294	H. suis	288:294	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	4	58	dep	H.	799:800	arg1	bind					807:810	bind	807:810	bind to	807:813	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	4	58	dep	H.	799:800	arg1	suis					802:805	H. suis	799:805	H. suis bind to	799:813	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	3	59	theme	human	539:543	arg1	mucins					561:566	human and pig gastric mucins	539:566	human and pig gastric mucins	539:566	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	6	60	theme	suis	1072:1075	arg1	binding					1077:1083	H. suis binding	1069:1083	H. suis binding to mucins	1069:1093	H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose.					
29638186	8	61	dep	H.	1505:1506	arg1	suis					1508:1511	H. suis	1505:1511	H. suis	1505:1511	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	2	62	theme	decreased	438:446	arg1	growth					448:453	decreased growth	438:453	decreased growth	438:453	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	4	63	theme	binding	635:641	arg1	optimum					643:649	The binding optimum	631:649	The binding optimum of H. pylori	631:662	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	5	64	theme	mucin	847:851	arg1	O-glycans					853:861	mucin O-glycans	847:861	mucin O-glycans from the porcine mucin	847:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	7	65	from	acidic	1346:1351	arg1	adhesion					1304:1311	adhesion	1304:1311	adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH	1304:1366	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	5	66	from	difference	948:957	arg1	glycosylation					962:974	glycosylation	962:974	glycosylation	962:974	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	9	67	theme	structures	1652:1661	arg1	Identification					1623:1636	Identification	1623:1636	Identification of the glycan structures H. suis	1623:1669	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	2	68	theme	disease	362:368	arg1	development					315:325	the development	311:325	the development of chronic gastritis, peptic ulcer disease and MALT lymphoma	311:386	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	5	69	theme	structures	1000:1009	arg1	present					1036:1042	present	1036:1042	present	1036:1042	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	70	theme	Mass	816:819	arg1	analysis					835:842	Mass spectrometric analysis	816:842	Mass spectrometric analysis of mucin O-glycans from the porcine mucin	816:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	2	71	theme	peptic	349:354	arg1	disease					362:368	peptic ulcer disease	349:368	peptic ulcer disease	349:368	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	4	72	dep	H.	654:655	arg1	pylori					657:662	H. pylori	654:662	H. pylori	654:662	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	7	73	attach	present	1197:1203	arg1	stomach					1212:1218	pig stomach	1208:1218	pig stomach	1208:1218	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	7	73	attach	present	1197:1203	arg2	glycolipids					1185:1195	the glycolipids	1181:1195	the glycolipids present in pig stomach	1181:1218	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	3	74	with	individuals	583:593	arg1	specificity					618:628	species dependent specificity	600:628	species dependent specificity	600:628	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	5	75	theme	individual	898:907	arg1	variation					909:917	individual variation	898:917	individual variation in binding	898:928	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	2	76	theme	MALT	374:377	arg1	lymphoma					379:386	MALT lymphoma	374:386	MALT lymphoma	374:386	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	1	77	located	found	241:245	arg2	species					233:239	the most prevalent non-Helicobacter pylori Helicobacter species	177:239	the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach	177:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	1	77	located	found	241:245	arg1	stomach					260:266	the human stomach	250:266	the human stomach	250:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	8	78	theme	acidic	1472:1477	arg1	charge					1479:1484	acidic charge	1472:1484	acidic charge	1472:1484	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29638186	3	79	theme	dependent	608:616	arg1	specificity					618:628	species dependent specificity	600:628	species dependent specificity	600:628	Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity.					
29638186	5	80	from	analysis	835:842	arg1	mucin					880:884	the porcine mucin	868:884	the porcine mucin	868:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	5	81	theme	porcine	872:878	arg1	mucin					880:884	the porcine mucin	868:884	the porcine mucin	868:884	Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples.					
29638186	7	82	from	present	1197:1203	arg1	stomach					1212:1218	pig stomach	1208:1218	pig stomach	1208:1218	Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates.					
29638186	9	83	theme	therapeutic	1719:1729	arg1	strategies					1731:1740	therapeutic strategies	1719:1740	therapeutic strategies alternative to antibiotics	1719:1767	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	1	84	theme	Helicobacter	220:231	arg1	species					233:239	the most prevalent non-Helicobacter pylori Helicobacter species	177:239	the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach	177:266	Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach.					
29638186	9	85	theme	alternative	1742:1752	arg1	strategies					1731:1740	therapeutic strategies	1719:1740	therapeutic strategies alternative to antibiotics	1719:1767	Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.					
29638186	4	86	theme	acidic	712:717	arg1	optimum					722:728	an acidic pH optimum	709:728	an acidic pH optimum	709:728	The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to.					
29638186	2	87	theme	gastritis	338:346	arg1	development					315:325	the development	311:325	the development of chronic gastritis, peptic ulcer disease and MALT lymphoma	311:386	In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers.					
29638186	8	88	dep	H.	1430:1431	arg1	suis					1433:1436	that H. suis	1425:1436	that H. suis bound to DNA (used as a proxy for acidic charge)	1425:1485	Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures.					
29732660	1	0	theme	defined	262:268	arg1	glycoforms					270:279	structurally defined glycoforms	249:279	structurally defined glycoforms	249:279	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	2	1	theme	sequential	488:497	arg1	extension					509:517	strictly controlled sequential enzymatic extension	468:517	strictly controlled sequential enzymatic extension	468:517	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	2	2	theme	such	375:378	arg1	structures					380:389	such structures	375:389	such structures	375:389	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	1	3	theme	glycoforms	270:279	arg1	lack					241:244	the lack	237:244	the lack of structurally defined glycoforms	237:279	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	2	4	theme	extension	324:332	arg1	strategy					341:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	4	5	theme	specific	756:763	arg1	antisera					765:772	specific antisera	756:772	specific antisera	756:772	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	4	6	theme	glycan	670:675	arg1	analysis					688:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	2	7	theme	synthesis/enzymatic	304:322	arg1	strategy					341:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	4	8	theme	Subsequent	659:668	arg1	analysis					688:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	1	9	theme	vital	76:80	arg1	modification					90:101	a vital protein modification	74:101	a vital protein modification involved in brain and muscle development	74:142	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	1	9	theme	vital	76:80	arg1	O Mannosylation					55:69	O Mannosylation	55:69	O Mannosylation	55:69	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	1	10	theme	O-mannosyl	180:189	arg1	glycans					191:197	O-mannosyl glycans	180:197	O-mannosyl glycans	180:197	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	1	11	theme	protein	82:88	arg1	modification					90:101	a vital protein modification	74:101	a vital protein modification involved in brain and muscle development	74:142	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	1	11	theme	protein	82:88	arg1	O Mannosylation					55:69	O Mannosylation	55:69	O Mannosylation	55:69	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	1	12	theme	glycans	191:197	arg1	relevance					167:175	the biological relevance	152:175	the biological relevance of O-mannosyl glycans	152:197	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	0	13	theme	Chemoenzymatic	7:20	arg1	Synthesis					22:30	Facile Chemoenzymatic Synthesis	0:30	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans	0:52	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.					
29732660	3	14	theme	identified	626:635	arg1	structures					647:656	identified mammalian structures	626:656	identified mammalian structures	626:656	In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.					
29732660	2	15	theme	convergent	415:424	arg1	syntheses					435:443	gram-scale convergent chemical syntheses	404:443	gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases	404:551	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	0	16	theme	Facile	0:5	arg1	Synthesis					22:30	Facile Chemoenzymatic Synthesis	0:30	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans	0:52	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.					
29732660	3	17	theme	mammalian	637:645	arg1	structures					647:656	identified mammalian structures	626:656	identified mammalian structures	626:656	In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.					
29732660	2	18	theme	gram-scale	404:413	arg1	syntheses					435:443	gram-scale convergent chemical syntheses	404:443	gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases	404:551	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	2	19	theme	controlled	477:486	arg1	extension					509:517	strictly controlled sequential enzymatic extension	468:517	strictly controlled sequential enzymatic extension	468:517	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	2	20	theme	SSEE	335:338	arg1	strategy					341:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	2	21	theme	scaffold	295:302	arg1	strategy					341:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	3	22	theme	O-mannosyl	567:576	arg1	glycans					578:584	45 O-mannosyl glycans	564:584	45 O-mannosyl glycans	564:584	In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.					
29732660	2	23	theme	scaffolds	454:462	arg1	syntheses					435:443	gram-scale convergent chemical syntheses	404:443	gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases	404:551	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	0	24	theme	Glycans	46:52	arg1	Synthesis					22:30	Facile Chemoenzymatic Synthesis	0:30	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans	0:52	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.					
29732660	4	25	theme	antisera	765:772	arg1	specificities					711:723	fine specificities	706:723	fine specificities of glycan-binding proteins and specific antisera	706:772	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	4	26	theme	proteins	743:750	arg1	specificities					711:723	fine specificities	706:723	fine specificities of glycan-binding proteins and specific antisera	706:772	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	0	27	theme	O-Mannosyl	35:44	arg1	Glycans					46:52	O-Mannosyl Glycans	35:52	O-Mannosyl Glycans	35:52	Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.					
29732660	4	28	theme	glycan-binding	728:741	arg1	proteins					743:750	glycan-binding proteins	728:750	glycan-binding proteins	728:750	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	3	29	theme	structures	647:656	arg1	majority					614:621	the majority	610:621	the majority of identified mammalian structures	610:656	In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.					
29732660	2	30	theme	extension	509:517	arg1	syntheses					435:443	gram-scale convergent chemical syntheses	404:443	gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases	404:551	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	2	31	theme	efficient	285:293	arg1	strategy					341:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy	282:348	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	1	32	theme	biological	156:165	arg1	relevance					167:175	the biological relevance	152:175	the biological relevance of O-mannosyl glycans	152:197	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
29732660	4	33	theme	microarray	677:686	arg1	analysis					688:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis	659:695	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	2	34	theme	chemical	426:433	arg1	syntheses					435:443	gram-scale convergent chemical syntheses	404:443	gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases	404:551	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	4	35	theme	fine	706:709	arg1	specificities					711:723	fine specificities	706:723	fine specificities of glycan-binding proteins and specific antisera	706:772	Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.					
29732660	2	36	theme	enzymatic	499:507	arg1	extension					509:517	strictly controlled sequential enzymatic extension	468:517	strictly controlled sequential enzymatic extension	468:517	An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases.					
29732660	1	37	dep	brain	115:119	arg1	development					132:142	development	132:142	development	132:142	O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms.					
31317190	9	0	theme	hepatocyte	1392:1401	arg1	factor					1410:1415	hepatocyte growth factor	1392:1415	hepatocyte growth factor	1392:1415	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	6	1	theme	lines	971:975	arg1	transcriptome					923:935	the transcriptome	919:935	the transcriptome	919:935	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	1	theme	lines	971:975	arg1	phenotype					945:953	the phenotype	941:953	the phenotype	941:953	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	8	2	from	genes	1280:1284	arg1	lines					1302:1306	the two cell lines	1289:1306	the two cell lines	1289:1306	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	3	3	theme	colorectal	364:373	arg1	cancer					375:380	colorectal cancer	364:380	colorectal cancer (CRC)	364:386	Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological and clinical significance remains unclear.					
31317190	3	3	theme	colorectal	364:373	arg1	CRC					383:385	CRC	383:385	CRC	383:385	Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological and clinical significance remains unclear.					
31317190	4	4	theme	Cancer	543:548	arg1	Atlas					557:561	The Cancer Genome Atlas	539:561	The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples	539:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	5	5	theme	low	680:682	arg1	expression					692:701	low ST6GAL1 expression	680:701	low ST6GAL1 expression	680:701	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	1	6	theme	disease	166:172	arg1	progression					174:184	disease progression	166:184	disease progression	166:184	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	6	7	theme	expression	871:880	arg1	impact					853:858	the impact	849:858	the impact of ST6GAL1 expression in experimental systems	849:904	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	8	theme	retroviral	998:1007	arg1	transduction					1009:1020	retroviral transduction	998:1020	retroviral transduction with ST6GAL1 cDNA	998:1038	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	8	9	theme	ST6GAL1	1173:1179	arg1	expression					1181:1190	Constitutive ST6GAL1 expression	1160:1190	Constitutive ST6GAL1 expression	1160:1190	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	0	10	from	Impact	0:5	arg1	types					82:86	different colon cancer cell types	54:86	different colon cancer cell types	54:86	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	5	11	theme	ST6GAL1	684:690	arg1	expression					692:701	low ST6GAL1 expression	680:701	low ST6GAL1 expression	680:701	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	10	12	theme	gene	1628:1631	arg1	profile					1644:1650	the gene expression profile	1624:1650	the gene expression profile	1624:1650	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	4	13	theme	CRC	629:631	arg1	hundreds					592:599	hundreds	592:599	hundreds of clinically characterized CRC and normal samples	592:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	8	14	theme	Constitutive	1160:1171	arg1	expression					1181:1190	Constitutive ST6GAL1 expression	1160:1190	Constitutive ST6GAL1 expression	1160:1190	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	9	15	dep	factor	1410:1415	arg1	ability					1422:1428	the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines	1418:1519	the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines	1418:1519	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	5	16	theme	expression	692:701	arg1	association					665:675	an association	662:675	an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival	662:831	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	10	17	theme	expression	1633:1642	arg1	profile					1644:1650	the gene expression profile	1624:1650	the gene expression profile	1624:1650	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	9	18	theme	growth	1403:1408	arg1	factor					1410:1415	hepatocyte growth factor	1392:1415	hepatocyte growth factor	1392:1415	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	4	19	theme	characterized	615:627	arg1	CRC					629:631	clinically characterized CRC	604:631	clinically characterized CRC	604:631	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	5	20	theme	mucinous	761:768	arg1	phenotype					770:778	mucinous phenotype	761:778	mucinous phenotype	761:778	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	4	21	theme	expression	504:513	arg1	relevance					483:491	the clinical relevance	470:491	the clinical relevance of ST6GAL1 expression in CRC	470:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	7	22	theme	CRC	1093:1095	arg1	transformation					1097:1110	CRC transformation	1093:1110	CRC transformation	1093:1110	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	8	23	theme	cell	1297:1300	arg1	lines					1302:1306	the two cell lines	1289:1306	the two cell lines	1289:1306	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	1	24	theme	progression	174:184	arg1	engines					155:161	engines	155:161	engines of disease progression	155:184	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	1	24	theme	progression	174:184	arg1	markers					143:149	tumor markers	137:149	tumor markers	137:149	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	6	25	from	impact	853:858	arg1	systems					898:904	experimental systems	885:904	experimental systems	885:904	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	4	26	from	CRC	518:520	arg1	relevance					483:491	the clinical relevance	470:491	the clinical relevance of ST6GAL1 expression in CRC	470:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	2	27	theme	cancer-associated	283:299	arg1	glycan					301:306	a cancer-associated glycan	281:306	a cancer-associated glycan	281:306	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	2	27	theme	cancer-associated	283:299	arg1	Siaα2,6Galβ1,4GlcNAc					201:220	The structure Siaα2,6Galβ1,4GlcNAc	187:220	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc)	187:233	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	3	28	theme	clinical	410:417	arg1	significance					419:430	their biological and clinical significance	389:430	their biological and clinical significance	389:430	Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological and clinical significance remains unclear.					
31317190	1	29	theme	Cancer-associated	89:105	arg1	structures					114:123	Cancer-associated glycan structures	89:123	Cancer-associated glycan structures	89:123	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	10	30	theme	glycosyltransferase	1596:1614	arg1	expression					1562:1571	the altered expression	1550:1571	the altered expression of a cancer-associated glycosyltransferase	1550:1614	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	8	31	theme	different	1270:1278	arg1	genes					1280:1284	different genes	1270:1284	different genes in the two cell lines	1270:1306	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	9	32	theme	tyrosine	1356:1363	arg1	phosphorylation					1365:1379	the tyrosine phosphorylation	1352:1379	the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines	1352:1519	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	4	33	from	expression	504:513	arg1	CRC					518:520	CRC	518:520	CRC	518:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	9	34	from	Matrigel	1490:1497	arg1	lines					1515:1519	the two cell lines	1502:1519	the two cell lines	1502:1519	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	1	35	theme	glycan	107:112	arg1	structures					114:123	Cancer-associated glycan structures	89:123	Cancer-associated glycan structures	89:123	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	9	36	theme	scratch	1462:1468	arg1	wound					1470:1474	a scratch wound	1460:1474	a scratch wound	1460:1474	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	3	37	theme	biological	395:404	arg1	significance					419:430	their biological and clinical significance	389:430	their biological and clinical significance	389:430	Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological and clinical significance remains unclear.					
31317190	4	38	theme	expression	573:582	arg1	data					584:587	mRNA expression data	568:587	mRNA expression data of hundreds of clinically characterized CRC and normal samples	568:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	7	39	theme	chromosomal	1113:1123	arg1	pathways					1081:1088	the two main pathways	1068:1088	the two main pathways of CRC transformation: chromosomal instability and MSI	1068:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	39	theme	chromosomal	1113:1123	arg1	instability					1125:1135	chromosomal instability	1113:1135	chromosomal instability	1113:1135	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	4	40	with	Atlas	557:561	arg1	data					584:587	mRNA expression data	568:587	mRNA expression data of hundreds of clinically characterized CRC and normal samples	568:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	0	41	theme	sialyltransferase	10:26	arg1	overexpression					36:49	sialyltransferase ST6GAL1 overexpression	10:49	sialyltransferase ST6GAL1 overexpression	10:49	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	4	42	theme	hundreds	592:599	arg1	data					584:587	mRNA expression data	568:587	mRNA expression data of hundreds of clinically characterized CRC and normal samples	568:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	7	43	theme	main	1076:1079	arg1	pathways					1081:1088	the two main pathways	1068:1088	the two main pathways of CRC transformation: chromosomal instability and MSI	1068:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	43	theme	main	1076:1079	arg1	instability					1125:1135	chromosomal instability	1113:1135	chromosomal instability	1113:1135	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	43	theme	main	1076:1079	arg1	MSI					1141:1143	MSI	1141:1143	MSI	1141:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	2	44	theme	structure	191:199	arg1	Sia6LacNAc					223:232	Sia6LacNAc	223:232	Sia6LacNAc	223:232	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	2	44	theme	structure	191:199	arg1	glycan					301:306	a cancer-associated glycan	281:306	a cancer-associated glycan	281:306	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	2	44	theme	structure	191:199	arg1	Siaα2,6Galβ1,4GlcNAc					201:220	The structure Siaα2,6Galβ1,4GlcNAc	187:220	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc)	187:233	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	7	45	dep	pathways	1081:1088	arg1	MSI					1141:1143	MSI	1141:1143	MSI	1141:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	45	dep	pathways	1081:1088	arg1	instability					1125:1135	chromosomal instability	1113:1135	chromosomal instability	1113:1135	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	45	dep	pathways	1081:1088	arg1	pathways					1081:1088	the two main pathways	1068:1088	the two main pathways of CRC transformation: chromosomal instability and MSI	1068:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	6	46	theme	cell	966:969	arg1	lines					971:975	the CRC cell lines	958:975	the CRC cell lines SW948 and SW48	958:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	46	theme	cell	966:969	arg1	SW48					987:990	SW48	987:990	SW48	987:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	46	theme	cell	966:969	arg1	SW948					977:981	SW948	977:981	SW948	977:981	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	4	47	from	relevance	483:491	arg1	CRC					518:520	CRC	518:520	CRC	518:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	4	48	theme	clinical	474:481	arg1	relevance					483:491	the clinical relevance	470:491	the clinical relevance of ST6GAL1 expression in CRC	470:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	2	49	theme	sialyltransferase	251:267	arg1	ST6GAL1					269:275	sialyltransferase ST6GAL1	251:275	sialyltransferase ST6GAL1	251:275	The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan.					
31317190	0	50	theme	overexpression	36:49	arg1	Impact					0:5	Impact	0:5	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.	0:87	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	6	51	theme	CRC	962:964	arg1	lines					971:975	the CRC cell lines	958:975	the CRC cell lines SW948 and SW48	958:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	51	theme	CRC	962:964	arg1	SW48					987:990	SW48	987:990	SW48	987:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	51	theme	CRC	962:964	arg1	SW948					977:981	SW948	977:981	SW948	977:981	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	5	52	theme	BRAF	742:745	arg1	mutations					747:755	BRAF mutations	742:755	BRAF mutations	742:755	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	4	53	theme	ST6GAL1	496:502	arg1	expression					504:513	ST6GAL1 expression	496:513	ST6GAL1 expression in CRC	496:520	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	0	54	theme	ST6GAL1	28:34	arg1	overexpression					36:49	sialyltransferase ST6GAL1 overexpression	10:49	sialyltransferase ST6GAL1 overexpression	10:49	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	9	55	dep	ability	1422:1428	arg1	grow					1433:1436	grow	1433:1436	to grow in soft agar	1430:1449	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	9	55	dep	ability	1422:1428	arg1	heal					1455:1458	heal	1455:1458	to heal a scratch wound	1452:1474	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	9	55	dep	ability	1422:1428	arg1	invade					1483:1488	invade	1483:1488	to invade Matrigel in the two cell lines	1480:1519	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	10	56	theme	altered	1554:1560	arg1	expression					1562:1571	the altered expression	1550:1571	the altered expression of a cancer-associated glycosyltransferase	1550:1614	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	0	57	theme	colon	64:68	arg1	types					82:86	different colon cancer cell types	54:86	different colon cancer cell types	54:86	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	4	58	theme	Genome	550:555	arg1	Atlas					557:561	The Cancer Genome Atlas	539:561	The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples	539:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	7	59	theme	cell	1049:1052	arg1	lines					1054:1058	The two cell lines	1041:1058	The two cell lines	1041:1058	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	6	60	theme	ST6GAL1	863:869	arg1	expression					871:880	ST6GAL1 expression	863:880	ST6GAL1 expression	863:880	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	61	theme	experimental	885:896	arg1	systems					898:904	experimental systems	885:904	experimental systems	885:904	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	0	62	theme	different	54:62	arg1	types					82:86	different colon cancer cell types	54:86	different colon cancer cell types	54:86	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	4	63	theme	mRNA	568:571	arg1	data					584:587	mRNA expression data	568:587	mRNA expression data of hundreds of clinically characterized CRC and normal samples	568:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	5	64	theme	microsatellite	708:721	arg1	instability					723:733	microsatellite instability	708:733	microsatellite instability (MSI)	708:739	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	5	64	theme	microsatellite	708:721	arg1	MSI					736:738	MSI	736:738	MSI	736:738	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	6	65	dep	lines	971:975	arg1	lines					971:975	the CRC cell lines	958:975	the CRC cell lines SW948 and SW48	958:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	65	dep	lines	971:975	arg1	SW48					987:990	SW48	987:990	SW48	987:990	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	6	65	dep	lines	971:975	arg1	SW948					977:981	SW948	977:981	SW948	977:981	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	7	66	theme	transformation	1097:1110	arg1	pathways					1081:1088	the two main pathways	1068:1088	the two main pathways of CRC transformation: chromosomal instability and MSI	1068:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	66	theme	transformation	1097:1110	arg1	instability					1125:1135	chromosomal instability	1113:1135	chromosomal instability	1113:1135	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	7	66	theme	transformation	1097:1110	arg1	MSI					1141:1143	MSI	1141:1143	MSI	1141:1143	The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively.					
31317190	5	67	with	association	665:675	arg1	instability					723:733	microsatellite instability	708:733	microsatellite instability (MSI)	708:739	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	5	67	with	association	665:675	arg1	survival					824:831	survival	824:831	survival	824:831	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	5	67	with	association	665:675	arg1	response					800:807	response	800:807	response to therapy	800:818	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	5	67	with	association	665:675	arg1	MSI					736:738	MSI	736:738	MSI	736:738	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	5	67	with	association	665:675	arg1	stage					793:797	stage	793:797	stage	793:797	We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival.					
31317190	1	68	theme	tumor	137:141	arg1	markers					143:149	tumor markers	137:149	tumor markers	137:149	Cancer-associated glycan structures can be both tumor markers and engines of disease progression.					
31317190	4	69	theme	normal	637:642	arg1	samples					644:650	normal samples	637:650	normal samples	637:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
31317190	9	70	theme	ST6GAL1	1309:1315	arg1	expression					1317:1326	ST6GAL1 expression	1309:1326	ST6GAL1 expression	1309:1326	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	9	71	theme	soft	1441:1444	arg1	agar					1446:1449	soft agar	1441:1449	soft agar	1441:1449	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	10	72	theme	cancer	1693:1698	arg1	manner					1717:1722	a cancer subtype-specific manner	1691:1722	a cancer subtype-specific manner	1691:1722	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	0	73	theme	cell	77:80	arg1	types					82:86	different colon cancer cell types	54:86	different colon cancer cell types	54:86	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	10	74	theme	subtype-specific	1700:1715	arg1	manner					1717:1722	a cancer subtype-specific manner	1691:1722	a cancer subtype-specific manner	1691:1722	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	6	75	with	transduction	1009:1020	arg1	cDNA					1035:1038	ST6GAL1 cDNA	1027:1038	ST6GAL1 cDNA	1027:1038	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	9	76	theme	cell	1510:1513	arg1	lines					1515:1519	the two cell lines	1502:1519	the two cell lines	1502:1519	ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines.					
31317190	6	77	theme	ST6GAL1	1027:1033	arg1	cDNA					1035:1038	ST6GAL1 cDNA	1027:1038	ST6GAL1 cDNA	1027:1038	To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA.					
31317190	0	78	theme	cancer	70:75	arg1	types					82:86	different colon cancer cell types	54:86	different colon cancer cell types	54:86	Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.					
31317190	8	79	from	changes	1227:1233	arg1	SW948					1238:1242	SW948	1238:1242	SW948	1238:1242	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	8	80	theme	transcriptomic	1212:1225	arg1	changes					1227:1233	much deeper transcriptomic changes	1200:1233	much deeper transcriptomic changes in SW948	1200:1242	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	10	81	theme	cancer-associated	1578:1594	arg1	glycosyltransferase					1596:1614	a cancer-associated glycosyltransferase	1576:1614	a cancer-associated glycosyltransferase	1576:1614	These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.					
31317190	8	82	theme	deeper	1205:1210	arg1	changes					1227:1233	much deeper transcriptomic changes	1200:1233	much deeper transcriptomic changes in SW948	1200:1242	Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines.					
31317190	4	83	theme	samples	644:650	arg1	hundreds					592:599	hundreds	592:599	hundreds of clinically characterized CRC and normal samples	592:650	To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples.					
29756448	2	0	theme	adjusted	378:385	arg1	strategy					387:394	The adjusted strategy	374:394	The adjusted strategy	374:394	The adjusted strategy rendered an easy chain elongation strategy.					
29756448	1	1	theme	oligosaccharides	356:371	arg1	assembly					340:347	the assembly	336:347	the assembly of the oligosaccharides	336:371	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	4	2	from	positions	726:734	arg1	derivatization					655:668	derivatization	655:668	derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue	655:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	2	from	positions	726:734	arg1	flexibility					610:620	flexibility	610:620	flexibility for the group transformation	610:649	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	3	theme	further	796:802	arg1	examination					804:814	the further examination	792:814	the further examination of the structure-activity relationship (SAR) of CS-E molecules	792:877	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	4	theme	residue	704:710	arg1	position					681:688	the N-2 position	673:688	the N-2 position of the GalNAc residue	673:710	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	4	theme	residue	704:710	arg1	positions					726:734	the O-1,5 positions	716:734	the O-1,5 positions of the GlcA residue	716:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	1	5	theme	characteristic	280:293	arg1	CS-E					305:308	CS-E	305:308	CS-E	305:308	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	1	5	theme	characteristic	280:293	arg1	groups					295:300	the characteristic groups	276:300	the characteristic groups of CS-E	276:308	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	4	6	theme	N-2	677:679	arg1	position					681:688	the N-2 position	673:688	the N-2 position of the GalNAc residue	673:710	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	2	7	theme	elongation	419:428	arg1	strategy					430:437	an easy chain elongation strategy	405:437	an easy chain elongation strategy	405:437	The adjusted strategy rendered an easy chain elongation strategy.					
29756448	4	8	theme	group	630:634	arg1	transformation					636:649	the group transformation	626:649	the group transformation	626:649	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	1	9	dep	introduced	315:324	arg1	different					226:234	different	226:234	different	226:234	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	4	10	theme	molecules	869:877	arg1	examination					804:814	the further examination	792:814	the further examination of the structure-activity relationship (SAR) of CS-E molecules	792:877	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	2	11	theme	chain	413:417	arg1	strategy					430:437	an easy chain elongation strategy	405:437	an easy chain elongation strategy	405:437	The adjusted strategy rendered an easy chain elongation strategy.					
29756448	4	12	used	used	572:575	arg2	disaccharide					555:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	12	used	used	572:575	arg2	block					593:597	the building block	580:597	the building block	580:597	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	13	theme	ready	775:779	arg1	access					781:786	ready access	775:786	ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules	775:877	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	2	14	theme	easy	408:411	arg1	strategy					430:437	an easy chain elongation strategy	405:437	an easy chain elongation strategy	405:437	The adjusted strategy rendered an easy chain elongation strategy.					
29756448	3	15	theme	elongation	451:460	arg1	steps					462:466	the elongation steps	447:466	the elongation steps	447:466	All of the elongation steps generated high yields with excellent glycosylation outcomes.					
29756448	1	16	theme	CS-E	305:308	arg1	CS-E					305:308	CS-E	305:308	CS-E	305:308	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	1	16	theme	CS-E	305:308	arg1	groups					295:300	the characteristic groups	276:300	the characteristic groups of CS-E	276:308	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	3	17	with	yields	483:488	arg1	outcomes					519:526	excellent glycosylation outcomes	495:526	excellent glycosylation outcomes	495:526	All of the elongation steps generated high yields with excellent glycosylation outcomes.					
29756448	4	18	from	position	681:688	arg1	derivatization					655:668	derivatization	655:668	derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue	655:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	18	from	position	681:688	arg1	flexibility					610:620	flexibility	610:620	flexibility for the group transformation	610:649	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	3	19	theme	excellent	495:503	arg1	outcomes					519:526	excellent glycosylation outcomes	495:526	excellent glycosylation outcomes	495:526	All of the elongation steps generated high yields with excellent glycosylation outcomes.					
29756448	0	20	theme	Sulfate-E	38:46	arg1	Precursors					71:80	Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors	26:80	Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors	26:80	An Approach to Synthesize Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors.					
29756448	1	21	theme	postglycosylation-transformation	183:214	arg1	strategy					216:223	a postglycosylation-transformation strategy	181:223	a postglycosylation-transformation strategy	181:223	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	3	22	theme	high	478:481	arg1	yields					483:488	high yields	478:488	high yields with excellent glycosylation outcomes	478:526	All of the elongation steps generated high yields with excellent glycosylation outcomes.					
29756448	0	23	theme	Chondroitin	26:36	arg1	Sulfate-E					38:46	Chondroitin Sulfate-E	26:46	Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors	26:80	An Approach to Synthesize Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors.					
29756448	0	23	theme	Chondroitin	26:36	arg1	CS-E					49:52	CS-E	49:52	CS-E	49:52	An Approach to Synthesize Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors.					
29756448	4	24	theme	CS-E	864:867	arg1	molecules					869:877	CS-E molecules	864:877	CS-E molecules	864:877	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	25	theme	residue	748:754	arg1	position					681:688	the N-2 position	673:688	the N-2 position of the GalNAc residue	673:710	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	25	theme	residue	748:754	arg1	positions					726:734	the O-1,5 positions	716:734	the O-1,5 positions of the GlcA residue	716:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	26	theme	building	584:591	arg1	disaccharide					555:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	26	theme	building	584:591	arg1	block					593:597	the building block	580:597	the building block	580:597	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	27	theme	GlcA	743:746	arg1	residue					748:754	the GlcA residue	739:754	the GlcA residue	739:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	1	28	from	all	241:243	arg1	different					226:234	different	226:234	different	226:234	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	4	29	theme	structure-activity	823:840	arg1	relationship					842:853	the structure-activity relationship	819:853	the structure-activity relationship (SAR)	819:859	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	29	theme	structure-activity	823:840	arg1	SAR					856:858	SAR	856:858	SAR	856:858	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	1	30	theme	chondroitin	123:133	arg1	CS-E					146:149	CS-E	146:149	CS-E	146:149	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	1	30	theme	chondroitin	123:133	arg1	sulfate-E					135:143	chondroitin sulfate-E	123:143	chondroitin sulfate-E (CS-E) oligosaccharides	123:167	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	1	31	theme	sulfate-E	135:143	arg1	oligosaccharides					152:167	chondroitin sulfate-E (CS-E) oligosaccharides	123:167	chondroitin sulfate-E (CS-E) oligosaccharides	123:167	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
29756448	3	32	theme	glycosylation	505:517	arg1	outcomes					519:526	excellent glycosylation outcomes	495:526	excellent glycosylation outcomes	495:526	All of the elongation steps generated high yields with excellent glycosylation outcomes.					
29756448	0	33	theme	Oligosaccharide	55:69	arg1	Precursors					71:80	Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors	26:80	Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors	26:80	An Approach to Synthesize Chondroitin Sulfate-E (CS-E) Oligosaccharide Precursors.					
29756448	4	34	theme	GalNAc	697:702	arg1	residue					704:710	the GalNAc residue	693:710	the GalNAc residue	693:710	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	35	theme	protected	545:553	arg1	disaccharide					555:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide	529:566	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	35	theme	protected	545:553	arg1	block					593:597	the building block	580:597	the building block	580:597	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	36	theme	relationship	842:853	arg1	examination					804:814	the further examination	792:814	the further examination of the structure-activity relationship (SAR) of CS-E molecules	792:877	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	4	37	theme	O-1,5	720:724	arg1	positions					726:734	the O-1,5 positions	716:734	the O-1,5 positions of the GlcA residue	716:754	An orthogonally protected disaccharide was used as the building block to provide flexibility for the group transformation and derivatization at the N-2 position of the GalNAc residue and the O-1,5 positions of the GlcA residue, thereby providing ready access for the further examination of the structure-activity relationship (SAR) of CS-E molecules.					
29756448	1	38	theme	traditional	252:262	arg1	approaches					264:273	the traditional approaches	248:273	the traditional approaches	248:273	An approach was developed to synthesize chondroitin sulfate-E (CS-E) oligosaccharides by adopting a postglycosylation-transformation strategy: different from all of the traditional approaches, the characteristic groups of CS-E were introduced following the assembly of the oligosaccharides.					
31040211	10	0	from	conformations	1842:1854	arg1	transitions					1810:1820	transitions	1810:1820	transitions from closed to open conformations during entry	1810:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	10	0	from	conformations	1842:1854	arg1	role					1785:1788	a role	1783:1788	a role of this receptor in transitions from closed to open conformations during entry	1783:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	6	1	theme	neutralization	1030:1043	arg1	assays					1069:1074	Temperature-dependent neutralization (e.g., virus breathing) assays	1008:1074	Temperature-dependent neutralization (e.g., virus breathing) assays	1008:1074	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	7	2	theme	high	1380:1383	arg1	temperatures					1385:1396	high temperatures	1380:1396	high temperatures	1380:1396	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	10	3	theme	scavenger	1738:1746	arg1	BI					1757:1758	scavenger receptor BI	1738:1758	scavenger receptor BI dependency	1738:1769	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	4	4	theme	indirect	766:773	arg1	protection					775:784	indirect protection	766:784	indirect protection of epitopes	766:796	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	3	5	theme	Huh7.5	605:610	arg1	infections					617:626	Huh7.5 cell infections	605:626	Huh7.5 cell infections	605:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	11	6	theme	similar	2007:2013	arg1	phenomena					2015:2023	similar phenomena	2007:2023	similar phenomena	2007:2023	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	1	7	theme	virus	171:175	arg1	infections					183:192	hepatitis C virus (HCV) infections	159:192	hepatitis C virus (HCV) infections	159:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	9	8	theme	open	1654:1657	arg1	conformations					1679:1691	open and closed envelope conformations	1654:1691	open and closed envelope conformations	1654:1691	In conclusion, our study indicates that HVR1 and glycans regulate HCV neutralization by shifting the equilibrium between open and closed envelope conformations.					
31040211	6	9	theme	envelope	1170:1177	arg1	conformation					1179:1190	envelope conformation	1170:1190	envelope conformation	1170:1190	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	9	10	theme	closed	1663:1668	arg1	conformations					1679:1691	open and closed envelope conformations	1654:1691	open and closed envelope conformations	1654:1691	In conclusion, our study indicates that HVR1 and glycans regulate HCV neutralization by shifting the equilibrium between open and closed envelope conformations.					
31040211	0	11	link	N-linked	27:34	arg1	glycans					36:42	N-linked glycans	27:42	N-linked glycans of hepatitis C	27:57	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	7	12	theme	increased	1341:1349	arg1	instability					1351:1361	increased instability	1341:1361	increased instability at acidic pH and high temperatures	1341:1396	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	5	13	theme	nonoverlapping	968:981	arg1	epitopes					998:1005	four nonoverlapping neutralization epitopes	963:1005	four nonoverlapping neutralization epitopes	963:1005	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	10	14	theme	BI	1757:1758	arg1	dependency					1760:1769	scavenger receptor BI dependency	1738:1769	scavenger receptor BI dependency	1738:1769	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	1	15	theme	infections	183:192	arg1	cases					150:154	About two million new cases	128:154	About two million new cases of hepatitis C virus (HCV) infections	128:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	4	16	theme	direct	813:818	arg1	shielding					827:835	direct steric shielding	813:835	direct steric shielding	813:835	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	5	17	theme	relative	938:945	arg1	strength					947:954	relative strength	938:954	relative strength	938:954	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	3	18	theme	HCV	552:554	arg1	strains					556:562	HCV strains H77(genotype 1a), J6(2a), and S52(3a)	552:600	HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections	552:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	18	theme	HCV	552:554	arg1	H77					564:566	H77	564:566	H77	564:566	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	18	theme	HCV	552:554	arg1	J6					582:583	J6	582:583	J6(2a)	582:587	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	18	theme	HCV	552:554	arg1	S52					594:596	S52	594:596	S52	594:596	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	4	19	with	consistent	750:759	arg1	protection					775:784	indirect protection	766:784	indirect protection of epitopes	766:796	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	1	20	theme	million	138:144	arg1	cases					150:154	About two million new cases	128:154	About two million new cases of hepatitis C virus (HCV) infections	128:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	8	21	theme	strong	1422:1427	arg1	correlation					1429:1439	a strong correlation	1420:1439	a strong correlation between neutralization sensitivity and scavenger receptor BI dependency	1420:1511	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	0	22	theme	C	57:57	arg1	glycans					36:42	N-linked glycans	27:42	N-linked glycans of hepatitis C	27:57	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	0	22	theme	C	57:57	arg1	region					14:19	Hypervariable region 1	0:21	Hypervariable region 1	0:21	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	10	23	theme	receptor	1798:1805	arg1	role					1785:1788	a role	1783:1788	a role of this receptor in transitions from closed to open conformations during entry	1783:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	7	24	from	pH	1373:1374	arg1	instability					1351:1361	increased instability	1341:1361	increased instability at acidic pH and high temperatures	1341:1396	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	11	25	theme	immune	2045:2050	arg1	evasion					2052:2058	immune evasion	2045:2058	immune evasion of other viruses	2045:2075	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	0	26	theme	virion	68:73	arg1	neutralization					75:88	virion neutralization	68:88	virion neutralization	68:88	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	1	27	theme	hepatitis	159:167	arg1	HCV					178:180	HCV	178:180	HCV	178:180	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	1	27	theme	hepatitis	159:167	arg1	virus					171:175	hepatitis C virus	159:175	hepatitis C virus (HCV) infections	159:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	6	28	theme	closed	1137:1142	arg1	conformation					1179:1190	envelope conformation	1170:1190	envelope conformation	1170:1190	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	2	29	gly	glycoprotein	384:395	arg1	glycoprotein					384:395	viral envelope glycoprotein	369:395	viral envelope glycoprotein E2	369:398	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	4	30	theme	neutralization	662:675	arg1	sensitivity					677:687	neutralization sensitivity	662:687	neutralization sensitivity	662:687	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	10	31	from	role	1785:1788	arg1	conformations					1842:1854	closed to open conformations	1827:1854	closed to open conformations during entry	1827:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	10	31	from	role	1785:1788	arg1	transitions					1810:1820	transitions	1810:1820	transitions from closed to open conformations during entry	1810:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	8	32	theme	scavenger	1480:1488	arg1	BI					1499:1500	scavenger receptor BI	1480:1500	scavenger receptor BI dependency	1480:1511	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	11	33	theme	glycoproteins	1925:1937	arg1	dynamics					1900:1907	structural dynamics	1889:1907	structural dynamics of HCV envelope glycoproteins	1889:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	2	34	theme	envelope	375:382	arg1	glycoprotein					384:395	viral envelope glycoprotein	369:395	viral envelope glycoprotein E2	369:398	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	0	35	theme	envelope	104:111	arg1	conformations					113:125	envelope conformations	104:125	envelope conformations	104:125	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	4	36	from	effects	651:657	arg1	sensitivity					677:687	neutralization sensitivity	662:687	neutralization sensitivity	662:687	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	8	37	theme	BI	1499:1500	arg1	dependency					1502:1511	scavenger receptor BI dependency	1480:1511	scavenger receptor BI dependency	1480:1511	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	0	38	theme	Hypervariable	0:12	arg1	region					14:19	Hypervariable region 1	0:21	Hypervariable region 1	0:21	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	11	39	theme	critical	1943:1950	arg1	implications					1952:1963	critical implications	1943:1963	critical implications for vaccine development	1943:1987	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	11	40	theme	envelope	1916:1923	arg1	glycoproteins					1925:1937	HCV envelope glycoproteins	1912:1937	HCV envelope glycoproteins	1912:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	7	41	theme	HVR1	1248:1251	arg1	removal					1237:1243	removal	1237:1243	removal of HVR1	1237:1251	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	2	42	theme	N-linked	445:452	arg1	glycans					454:460	N-linked glycans	445:460	N-linked glycans	445:460	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	43	theme	E2	397:398	arg1	features					357:364	molecular features	347:364	molecular features	347:364	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	43	theme	E2	397:398	arg1	glycans					454:460	N-linked glycans	445:460	N-linked glycans	445:460	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	43	theme	E2	397:398	arg1	region					425:430	hypervariable region 1	411:432	hypervariable region 1 (HVR1)	411:439	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	0	44	theme	N-linked	27:34	arg1	glycans					36:42	N-linked glycans	27:42	N-linked glycans of hepatitis C	27:57	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	10	45	theme	closed	1827:1832	arg1	conformations					1842:1854	closed to open conformations	1827:1854	closed to open conformations during entry	1827:1867	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	11	46	gly	glycoproteins	1925:1937	arg1	glycoproteins					1925:1937	HCV envelope glycoproteins	1912:1937	HCV envelope glycoproteins	1912:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	3	47	theme	genotype	568:575	arg1	H77					564:566	H77	564:566	H77	564:566	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	47	theme	genotype	568:575	arg1	1a					577:578	genotype 1a	568:578	genotype 1a	568:578	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	11	48	theme	HCV	1912:1914	arg1	glycoproteins					1925:1937	HCV envelope glycoproteins	1912:1937	HCV envelope glycoproteins	1912:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	3	49	theme	large	480:484	arg1	variation					486:494	large variation	480:494	large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections	480:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	9	50	theme	HCV	1599:1601	arg1	neutralization					1603:1616	HCV neutralization	1599:1616	HCV neutralization	1599:1616	In conclusion, our study indicates that HVR1 and glycans regulate HCV neutralization by shifting the equilibrium between open and closed envelope conformations.					
31040211	4	51	theme	epitopes	789:796	arg1	protection					775:784	indirect protection	766:784	indirect protection of epitopes	766:796	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	6	52	theme	Temperature-dependent	1008:1028	arg1	neutralization					1030:1043	Temperature-dependent neutralization	1008:1043	Temperature-dependent neutralization (e.g., virus breathing) assays	1008:1074	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	10	53	dep	closed	1827:1832	arg1	open					1837:1840	open	1837:1840	open	1837:1840	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	10	53	dep	closed	1827:1832	arg1	to					1834:1835	to	1834:1835	to	1834:1835	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	10	54	theme	receptor	1748:1755	arg1	BI					1757:1758	scavenger receptor BI	1738:1758	scavenger receptor BI dependency	1738:1769	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	2	55	theme	HCV	297:299	arg1	evades					301:306	HCV evades	297:306	HCV evades	297:306	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	6	56	theme	protective	1105:1114	arg1	glycans					1116:1122	protective glycans	1105:1122	protective glycans	1105:1122	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	3	57	from	variation	486:494	arg1	effects					503:509	the effects	499:509	the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections	499:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	1	58	theme	C	169:169	arg1	HCV					178:180	HCV	178:180	HCV	178:180	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	1	58	theme	C	169:169	arg1	virus					171:175	hepatitis C virus	159:175	hepatitis C virus (HCV) infections	159:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	3	59	theme	cell	612:615	arg1	infections					617:626	Huh7.5 cell infections	605:626	Huh7.5 cell infections	605:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	60	theme	E2	534:535	arg1	glycans					537:543	individual E2 glycans	523:543	individual E2 glycans	523:543	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	5	61	from	type	901:904	arg1	effect					850:855	the effect	846:855	the effect of removing each glycan	846:879	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	5	61	from	type	901:904	arg1	similar					885:891	similar	885:891	similar	885:891	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	3	62	dep	strains	556:562	arg1	strains					556:562	HCV strains H77(genotype 1a), J6(2a), and S52(3a)	552:600	HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections	552:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	H77					564:566	H77	564:566	H77	564:566	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	J6					582:583	J6	582:583	J6(2a)	582:587	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	2a					585:586	2a	585:586	2a	585:586	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	3a					598:599	3a	598:599	3a	598:599	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	1a					577:578	genotype 1a	568:578	genotype 1a	568:578	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	3	62	dep	strains	556:562	arg1	S52					594:596	S52	594:596	S52	594:596	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	7	63	theme	acidic	1366:1371	arg1	pH					1373:1374	acidic pH	1366:1374	acidic pH	1366:1374	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	9	64	theme	envelope	1670:1677	arg1	conformations					1679:1691	open and closed envelope conformations	1654:1691	open and closed envelope conformations	1654:1691	In conclusion, our study indicates that HVR1 and glycans regulate HCV neutralization by shifting the equilibrium between open and closed envelope conformations.					
31040211	5	65	theme	neutralization	983:996	arg1	epitopes					998:1005	four nonoverlapping neutralization epitopes	963:1005	four nonoverlapping neutralization epitopes	963:1005	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	11	66	contain	has	1939:1941	arg1	importance					1875:1884	This importance	1870:1884	This importance of structural dynamics of HCV envelope glycoproteins	1870:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	11	66	contain	has	1939:1941	arg2	implications					1952:1963	critical implications	1943:1963	critical implications for vaccine development	1943:1987	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	11	67	theme	other	2063:2067	arg1	viruses					2069:2075	other viruses	2063:2075	other viruses	2063:2075	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	5	68	dep	type	901:904	arg1	sensitizing					921:931	sensitizing	921:931	sensitizing	921:931	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	5	68	dep	type	901:904	arg1	protective					907:916	protective	907:916	protective	907:916	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	5	68	dep	type	901:904	arg1	both					893:896	both	893:896	both	893:896	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	11	69	theme	viruses	2069:2075	arg1	evasion					2052:2058	immune evasion	2045:2058	immune evasion of other viruses	2045:2075	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	6	70	theme	difficult	1145:1153	arg1	conformation					1179:1190	envelope conformation	1170:1190	envelope conformation	1170:1190	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	4	71	theme	steric	820:825	arg1	shielding					827:835	direct steric shielding	813:835	direct steric shielding	813:835	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	7	72	theme	glycan	1310:1315	arg1	status					1317:1322	glycan status	1310:1322	glycan status	1310:1322	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	7	73	theme	stabilizing	1198:1208	arg1	effect					1210:1215	This stabilizing effect	1193:1215	This stabilizing effect	1193:1215	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	7	74	from	temperatures	1385:1396	arg1	instability					1351:1361	increased instability	1341:1361	increased instability at acidic pH and high temperatures	1341:1396	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	11	75	theme	vaccine	1969:1975	arg1	development					1977:1987	vaccine development	1969:1987	vaccine development	1969:1987	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	1	76	theme	new	146:148	arg1	cases					150:154	About two million new cases	128:154	About two million new cases of hepatitis C virus (HCV) infections	128:192	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	8	77	theme	neutralization	1449:1462	arg1	sensitivity					1464:1474	neutralization sensitivity	1449:1474	neutralization sensitivity	1449:1474	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	3	78	theme	individual	523:532	arg1	glycans					537:543	individual E2 glycans	523:543	individual E2 glycans	523:543	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	8	79	theme	receptor	1490:1497	arg1	BI					1499:1500	scavenger receptor BI	1480:1500	scavenger receptor BI dependency	1480:1511	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	7	80	with	consistent	1325:1334	arg1	instability					1351:1361	increased instability	1341:1361	increased instability at acidic pH and high temperatures	1341:1396	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	7	81	theme	closed	1272:1277	arg1	conformations					1279:1291	closed conformations	1272:1291	closed conformations	1272:1291	This stabilizing effect was hierarchical as removal of HVR1 fully destabilized closed conformations, irrespective of glycan status, consistent with increased instability at acidic pH and high temperatures.					
31040211	10	82	dep	appears	1710:1716	arg1	linked					1726:1731	linked	1726:1731	linked	1726:1731	This regulation appears tightly linked with scavenger receptor BI dependency, suggesting a role of this receptor in transitions from closed to open conformations during entry.					
31040211	2	83	theme	viral	369:373	arg1	glycoprotein					384:395	viral envelope glycoprotein	369:395	viral envelope glycoprotein E2	369:398	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	8	84	theme	viral	1520:1524	arg1	entry					1526:1530	viral entry	1520:1530	viral entry	1520:1530	Finally, we observed a strong correlation between neutralization sensitivity and scavenger receptor BI dependency during viral entry.					
31040211	2	85	link	N-linked	445:452	arg1	glycans					454:460	N-linked glycans	445:460	N-linked glycans	445:460	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	5	86	from	strength	947:954	arg1	effect					850:855	the effect	846:855	the effect of removing each glycan	846:879	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	5	86	from	strength	947:954	arg1	similar					885:891	similar	885:891	similar	885:891	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	2	87	theme	hypervariable	411:423	arg1	region					425:430	hypervariable region 1	411:432	hypervariable region 1 (HVR1)	411:439	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	87	theme	hypervariable	411:423	arg1	HVR1					435:438	HVR1	435:438	HVR1	435:438	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	4	88	theme	glycan-mediated	635:649	arg1	effects					651:657	glycan-mediated effects	635:657	glycan-mediated effects on neutralization sensitivity	635:687	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	3	89	from	strains	556:562	arg1	infections					617:626	Huh7.5 cell infections	605:626	Huh7.5 cell infections	605:626	Here, we observe large variation in the effects of removing individual E2 glycans across HCV strains H77(genotype 1a), J6(2a), and S52(3a) in Huh7.5 cell infections.					
31040211	2	90	theme	molecular	347:355	arg1	features					357:364	molecular features	347:364	molecular features	347:364	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	90	theme	molecular	347:355	arg1	glycans					454:460	N-linked glycans	445:460	N-linked glycans	445:460	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	2	90	theme	molecular	347:355	arg1	region					425:430	hypervariable region 1	411:432	hypervariable region 1 (HVR1)	411:439	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	11	91	theme	structural	1889:1898	arg1	dynamics					1900:1907	structural dynamics	1889:1907	structural dynamics of HCV envelope glycoproteins	1889:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	4	92	theme	neutralization	725:738	arg1	data					740:743	neutralization data	725:743	neutralization data	725:743	Also, glycan-mediated effects on neutralization sensitivity were completely HVR1-dependent, and neutralization data were consistent with indirect protection of epitopes, as opposed to direct steric shielding.					
31040211	2	93	theme	glycoprotein	384:395	arg1	E2					397:398	viral envelope glycoprotein E2	369:398	viral envelope glycoprotein E2	369:398	However, this effort has proven challenging because HCV evades neutralizing antibodies (NAbs) through molecular features of viral envelope glycoprotein E2, including hypervariable region 1 (HVR1) and N-linked glycans.					
31040211	0	94	theme	hepatitis	47:55	arg1	C					57:57	hepatitis C	47:57	hepatitis C	47:57	Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations.					
31040211	6	95	theme	virus	1052:1056	arg1	breathing					1058:1066	virus breathing	1052:1066	virus breathing	1052:1066	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	1	96	theme	urgent	218:223	arg1	need					225:228	the urgent need	214:228	the urgent need for a vaccine	214:242	About two million new cases of hepatitis C virus (HCV) infections annually underscore the urgent need for a vaccine.					
31040211	11	97	theme	dynamics	1900:1907	arg1	importance					1875:1884	This importance	1870:1884	This importance of structural dynamics of HCV envelope glycoproteins	1870:1937	This importance of structural dynamics of HCV envelope glycoproteins has critical implications for vaccine development and suggests that similar phenomena could contribute to immune evasion of other viruses.					
31040211	6	98	dep	breathing	1058:1066	arg1	e.g.					1046:1049	e.g.	1046:1049	e.g.	1046:1049	Temperature-dependent neutralization (e.g., virus breathing) assays indicated that both HVR1 and protective glycans stabilized a closed, difficult to neutralize, envelope conformation.					
31040211	5	99	from	similar	885:891	arg1	strength					947:954	relative strength	938:954	relative strength	938:954	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
31040211	5	99	from	similar	885:891	arg1	type					901:904	type	901:904	type (protective or sensitizing)	901:932	Indeed, the effect of removing each glycan was similar both in type (protective or sensitizing) and relative strength across four nonoverlapping neutralization epitopes.					
30372068	6	0	theme	surface	1052:1058	arg1	hydrophobicity					1060:1073	surface hydrophobicity	1052:1073	surface hydrophobicity	1052:1073	The glycation with increasing the DG led to a progressive decrease in solubility and surface hydrophobicity but remarkably increased the magnitude of ζ-potential.					
30372068	10	1	theme	outstanding	2049:2059	arg1	stabilization					2071:2083	an outstanding Pickering stabilization	2046:2083	an outstanding Pickering stabilization	2046:2083	It was also confirmed that once the glycated SG adsorbed at the interface, it would readily dissociated into subunits; the dissociated [AB] subunits exhibited an outstanding Pickering stabilization.					
30372068	6	2	from	decrease	1025:1032	arg1	hydrophobicity					1060:1073	surface hydrophobicity	1052:1073	surface hydrophobicity	1052:1073	The glycation with increasing the DG led to a progressive decrease in solubility and surface hydrophobicity but remarkably increased the magnitude of ζ-potential.					
30372068	6	2	from	decrease	1025:1032	arg1	solubility					1037:1046	solubility	1037:1046	solubility	1037:1046	The glycation with increasing the DG led to a progressive decrease in solubility and surface hydrophobicity but remarkably increased the magnitude of ζ-potential.					
30372068	7	3	from	dissociation	1230:1241	arg1	level					1280:1284	the quaternary level	1265:1284	the quaternary level	1265:1284	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	9	4	theme	bridged	1868:1874	arg1	emulsions					1876:1884	bridged emulsions	1868:1884	bridged emulsions	1868:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	8	5	theme	bridged	1611:1617	arg1	emulsions					1619:1627	bridged emulsions	1611:1627	bridged emulsions	1611:1627	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	7	6	theme	gradual	1222:1228	arg1	dissociation					1230:1241	a gradual dissociation	1220:1241	a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits)	1220:1315	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	4	7	theme	physicochemical	620:634	arg1	properties					651:660	The physicochemical and structural properties	616:660	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35%	616:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	0	8	theme	Molecular	0:8	arg1	Mechanism					10:18	Molecular Mechanism	0:18	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.	0:120	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	1	9	theme	effective	180:188	arg1	strategy					190:197	an effective strategy	177:197	an effective strategy to improve the emulsifying properties of plant storage globulins	177:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	1	9	theme	effective	180:188	arg1	Glycation					122:130	Glycation	122:130	Glycation with carbohydrates	122:149	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	4	10	theme	structural	640:649	arg1	properties					651:660	The physicochemical and structural properties	616:660	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35%	616:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	6	11	theme	ζ-potential	1117:1127	arg1	magnitude					1104:1112	the magnitude	1100:1112	the magnitude of ζ-potential	1100:1127	The glycation with increasing the DG led to a progressive decrease in solubility and surface hydrophobicity but remarkably increased the magnitude of ζ-potential.					
30372068	10	12	theme	glycated	1923:1930	arg1	SG					1932:1933	the glycated SG	1919:1933	the glycated SG adsorbed at the interface	1919:1959	It was also confirmed that once the glycated SG adsorbed at the interface, it would readily dissociated into subunits; the dissociated [AB] subunits exhibited an outstanding Pickering stabilization.					
30372068	3	13	theme	acidic	557:562	arg1	polypeptides					582:593	both the acidic (A) and basic (B) polypeptides	548:593	polypeptides	582:593	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	9	14	theme	emulsification	1683:1696	arg1	efficiency					1698:1707	the emulsification efficiency	1679:1707	the emulsification efficiency of SG	1679:1713	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	7	15	theme	[AB	1302:1304	arg1	subunits					1307:1314	different [AB] subunits	1292:1314	different [AB] subunits	1292:1314	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	7	16	theme	latter	1138:1143	arg1	scattering					1145:1154	Dynamic latter scattering and spectroscopic results	1130:1180	scattering	1145:1154	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	7	17	theme	DG-dependent	1323:1334	arg1	way					1336:1338	a DG-dependent way	1321:1338	a DG-dependent way	1321:1338	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	4	18	theme	DG	700:701	arg1	values					703:708	different DG values	690:708	different DG values of 0-35%	690:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	0	19	theme	Glycinin	67:74	arg1	Efficiency					49:58	Emulsification Efficiency	34:58	Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide	34:119	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	5	20	theme	0.3	850:852	arg1	concentration					868:880	a protein concentration	858:880	a protein concentration in the aqueous phase, produced using microfluidization as the emusification process	858:964	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	5	20	theme	0.3	850:852	arg1	fraction					838:845	an oil fraction	831:845	an oil fraction of 0.3	831:852	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	8	21	theme	emulsification	1438:1451	arg1	efficiency					1453:1462	the emulsification efficiency	1434:1462	the emulsification efficiency	1434:1462	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	4	22	theme	SG	674:675	arg1	samples					677:683	glycated SG samples	665:683	glycated SG samples with different DG values of 0-35%	665:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	0	23	theme	Soy	94:96	arg1	Polysaccharide					106:119	Soy Soluble Polysaccharide	94:119	Soy Soluble Polysaccharide	94:119	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	8	24	dep	interface	1584:1592	arg1	the					1580:1582	the	1580:1582	the	1580:1582	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	11	25	theme	emulsifying	2204:2214	arg1	properties					2216:2225	emulsifying properties	2204:2225	emulsifying properties of oligomeric globulins	2204:2249	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	9	26	theme	enhanced	1772:1779	arg1	flexibility					1794:1804	the enhanced conformation flexibility	1768:1804	the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions	1768:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	7	27	theme	spectroscopic	1160:1172	arg1	results					1174:1180	Dynamic latter scattering and spectroscopic results	1130:1180	results	1174:1180	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	6	28	theme	progressive	1013:1023	arg1	decrease					1025:1032	a progressive decrease	1011:1032	a progressive decrease in solubility and surface hydrophobicity	1011:1073	The glycation with increasing the DG led to a progressive decrease in solubility and surface hydrophobicity but remarkably increased the magnitude of ζ-potential.					
30372068	2	29	theme	soy	456:458	arg1	glycinin					460:467	soy glycinin	456:467	soy glycinin (SG)	456:472	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	2	29	theme	soy	456:458	arg1	SG					470:471	SG	470:471	SG	470:471	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	8	30	theme	droplet	1509:1515	arg1	flocculation					1517:1528	the droplet flocculation	1505:1528	the droplet flocculation	1505:1528	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	4	31	theme	%	717:717	arg1	values					703:708	different DG values	690:708	different DG values of 0-35%	690:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	11	32	theme	molecular	2160:2168	arg1	mechanism					2170:2178	the molecular mechanism	2156:2178	the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides	2156:2287	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	1	33	dep	strategy	190:197	arg1	improve					202:208	improve	202:208	to improve the emulsifying properties of plant storage globulins	199:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	7	34	theme	Dynamic	1130:1136	arg1	scattering					1145:1154	Dynamic latter scattering and spectroscopic results	1130:1180	scattering	1145:1154	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	0	35	theme	Emulsification	34:47	arg1	Efficiency					49:58	Emulsification Efficiency	34:58	Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide	34:119	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	2	36	with	glycation	351:359	arg1	SSPS					394:397	soy soluble polysaccharide (SSPS)	366:398	soy soluble polysaccharide (SSPS)	366:398	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	3	37	theme	similar	600:606	arg1	extent					608:613	a similar extent	598:613	a similar extent	598:613	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	5	38	theme	unglycated	769:778	arg1	SG					793:794	unglycated and glycated SG	769:794	unglycated and glycated SG	769:794	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	8	39	theme	emulsions	1619:1627	arg1	formation					1598:1606	formation	1598:1606	formation	1598:1606	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	8	39	theme	emulsions	1619:1627	arg1	interface					1584:1592	interface	1584:1592	interface	1584:1592	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	7	40	theme	SG	1259:1260	arg1	dissociation					1230:1241	a gradual dissociation	1220:1241	a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits)	1220:1315	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	10	41	theme	[AB	2022:2024	arg1	subunits					2027:2034	the dissociated [AB] subunits	2006:2034	the dissociated [AB] subunits	2006:2034	It was also confirmed that once the glycated SG adsorbed at the interface, it would readily dissociated into subunits; the dissociated [AB] subunits exhibited an outstanding Pickering stabilization.					
30372068	8	42	theme	proteins	1568:1575	arg1	adsorption					1550:1559	the adsorption	1546:1559	the adsorption of the proteins at the interface and formation of bridged emulsions	1546:1627	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	2	43	theme	polysaccharide	378:391	arg1	SSPS					394:397	soy soluble polysaccharide (SSPS)	366:398	soy soluble polysaccharide (SSPS)	366:398	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	1	44	theme	storage	246:252	arg1	globulins					254:262	plant storage globulins	240:262	plant storage globulins	240:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	9	45	theme	facilitated	1843:1853	arg1	formation					1855:1863	facilitated formation	1843:1863	the [AB] subunit level as well as facilitated formation of bridged emulsions	1809:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	2	46	theme	soy	366:368	arg1	SSPS					394:397	soy soluble polysaccharide (SSPS)	366:398	soy soluble polysaccharide (SSPS)	366:398	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	4	47	with	samples	677:683	arg1	values					703:708	different DG values	690:708	different DG values of 0-35%	690:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	0	48	with	Glycation	79:87	arg1	Polysaccharide					106:119	Soy Soluble Polysaccharide	94:119	Soy Soluble Polysaccharide	94:119	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	11	49	theme	new	2136:2138	arg1	knowledge					2140:2148	new knowledge	2136:2148	new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides	2136:2287	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	9	50	theme	emulsions	1876:1884	arg1	formation					1855:1863	facilitated formation	1843:1863	the [AB] subunit level as well as facilitated formation of bridged emulsions	1809:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	9	50	theme	emulsions	1876:1884	arg1	level					1826:1830	the [AB] subunit level	1809:1830	the [AB] subunit level as well as facilitated formation of bridged emulsions	1809:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	5	51	theme	oil	834:836	arg1	fraction					838:845	an oil fraction	831:845	an oil fraction of 0.3	831:852	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	7	52	theme	secondary	1382:1390	arg1	structure					1392:1400	secondary structure	1382:1400	secondary structure	1382:1400	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	9	53	from	formation	1855:1863	arg1	flexibility					1794:1804	the enhanced conformation flexibility	1768:1804	the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions	1768:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	11	54	theme	globulins	2241:2249	arg1	properties					2216:2225	emulsifying properties	2204:2225	emulsifying properties of oligomeric globulins	2204:2249	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	3	55	dep	basic	572:576	arg1	B					579:579	B	579:579	B	579:579	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	7	56	theme	[AB	1363:1365	arg1	subunits					1368:1375	their tertiary ([AB] subunits)	1347:1376	their tertiary ([AB] subunits)	1347:1376	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	8	57	from	interface	1584:1592	arg1	adsorption					1550:1559	the adsorption	1546:1559	the adsorption of the proteins at the interface and formation of bridged emulsions	1546:1627	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	2	58	theme	glycinin	460:467	arg1	efficiency					442:451	the emulsification efficiency	423:451	the emulsification efficiency of soy glycinin (SG)	423:472	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	7	59	theme	tertiary	1353:1360	arg1	subunits					1368:1375	their tertiary ([AB] subunits)	1347:1376	their tertiary ([AB] subunits)	1347:1376	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	3	60	theme	basic	572:576	arg1	polypeptides					582:593	both the acidic (A) and basic (B) polypeptides	548:593	polypeptides	582:593	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	3	61	dep	polypeptides	582:593	arg1	both					548:551	both	548:551	both	548:551	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	9	62	theme	efficiency	1698:1707	arg1	improvement					1664:1674	the improvement	1660:1674	the improvement of the emulsification efficiency of SG by the glycation with SSPS	1660:1740	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	5	63	theme	protein	860:866	arg1	concentration					868:880	a protein concentration	858:880	a protein concentration in the aqueous phase, produced using microfluidization as the emusification process	858:964	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	9	64	theme	SG	1712:1713	arg1	efficiency					1698:1707	the emulsification efficiency	1679:1707	the emulsification efficiency of SG	1679:1713	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	4	65	theme	samples	677:683	arg1	properties					651:660	The physicochemical and structural properties	616:660	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35%	616:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	8	66	from	formation	1598:1606	arg1	adsorption					1550:1559	the adsorption	1546:1559	the adsorption of the proteins at the interface and formation of bridged emulsions	1546:1627	Besides the emulsification efficiency, the glycation progressively accelerated the droplet flocculation and facilitated the adsorption of the proteins at the interface and formation of bridged emulsions.					
30372068	9	67	from	level	1826:1830	arg1	flexibility					1794:1804	the enhanced conformation flexibility	1768:1804	the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions	1768:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	4	68	theme	different	690:698	arg1	values					703:708	different DG values	690:708	different DG values of 0-35%	690:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	9	69	with	glycation	1722:1730	arg1	SSPS					1737:1740	SSPS	1737:1740	SSPS	1737:1740	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	5	70	from	fraction	838:845	arg1	phase					897:901	the aqueous phase	885:901	the aqueous phase	885:901	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	4	71	theme	glycated	665:672	arg1	samples					677:683	glycated SG samples	665:683	glycated SG samples with different DG values of 0-35%	665:717	The physicochemical and structural properties of glycated SG samples with different DG values of 0-35% were characterized.					
30372068	0	72	theme	Soluble	98:104	arg1	Polysaccharide					106:119	Soy Soluble Polysaccharide	94:119	Soy Soluble Polysaccharide	94:119	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	3	73	dep	acidic	557:562	arg1	A					565:565	A	565:565	A	565:565	The glycation occurred in both the acidic (A) and basic (B) polypeptides to a similar extent.					
30372068	11	74	theme	oligomeric	2230:2239	arg1	globulins					2241:2249	oligomeric globulins	2230:2249	oligomeric globulins	2230:2249	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	2	75	theme	-dependent	503:512	arg1	manner					514:519	a degree-of-glycation (DG)-dependent manner	477:519	a degree-of-glycation (DG)-dependent manner	477:519	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	1	76	with	Glycation	122:130	arg1	carbohydrates					137:149	carbohydrates	137:149	carbohydrates	137:149	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	5	77	theme	aqueous	889:895	arg1	phase					897:901	the aqueous phase	885:901	the aqueous phase	885:901	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	7	78	theme	different	1292:1300	arg1	subunits					1307:1314	different [AB] subunits	1292:1314	different [AB] subunits	1292:1314	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	9	79	theme	conformation	1781:1792	arg1	flexibility					1794:1804	the enhanced conformation flexibility	1768:1804	the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions	1768:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	5	80	theme	SG	793:794	arg1	properties					755:764	The emulsifying properties	739:764	The emulsifying properties of unglycated and glycated SG	739:794	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	5	81	theme	glycated	784:791	arg1	SG					793:794	unglycated and glycated SG	769:794	unglycated and glycated SG	769:794	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	5	82	from	concentration	868:880	arg1	phase					897:901	the aqueous phase	885:901	the aqueous phase	885:901	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	2	83	theme	emulsification	427:440	arg1	efficiency					442:451	the emulsification efficiency	423:451	the emulsification efficiency of soy glycinin (SG)	423:472	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	5	84	theme	emulsifying	743:753	arg1	properties					755:764	The emulsifying properties	739:764	The emulsifying properties of unglycated and glycated SG	739:794	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	1	85	theme	emulsifying	214:224	arg1	properties					226:235	the emulsifying properties	210:235	the emulsifying properties of plant storage globulins	210:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	7	86	theme	quaternary	1269:1278	arg1	level					1280:1284	the quaternary level	1265:1284	the quaternary level	1265:1284	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	9	87	theme	[AB	1813:1815	arg1	level					1826:1830	the [AB] subunit level	1809:1830	the [AB] subunit level as well as facilitated formation of bridged emulsions	1809:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	11	88	theme	properties	2216:2225	arg1	modification					2188:2199	the modification	2184:2199	the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides	2184:2287	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	0	89	theme	Soy	63:65	arg1	Glycinin					67:74	Soy Glycinin	63:74	Soy Glycinin	63:74	Molecular Mechanism for Improving Emulsification Efficiency of Soy Glycinin by Glycation with Soy Soluble Polysaccharide.					
30372068	7	90	from	level	1280:1284	arg1	SG					1259:1260	the 11S-form SG	1246:1260	the 11S-form SG at the quaternary level (into different [AB] subunits)	1246:1315	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	7	90	from	level	1280:1284	arg1	dissociation					1230:1241	a gradual dissociation	1220:1241	a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits)	1220:1315	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	9	91	theme	subunit	1818:1824	arg1	level					1826:1830	the [AB] subunit level	1809:1830	the [AB] subunit level as well as facilitated formation of bridged emulsions	1809:1884	The results demonstrated that the improvement of the emulsification efficiency of SG by the glycation with SSPS was largely attributed to the enhanced conformation flexibility at the [AB] subunit level as well as facilitated formation of bridged emulsions.					
30372068	5	92	theme	emusification	944:956	arg1	microfluidization					919:935	microfluidization	919:935	microfluidization	919:935	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	5	92	theme	emusification	944:956	arg1	process					958:964	the emusification process	940:964	the emusification process	940:964	The emulsifying properties of unglycated and glycated SG were performed on the emulsions at an oil fraction of 0.3 and a protein concentration in the aqueous phase, produced using microfluidization as the emusification process.					
30372068	7	93	theme	11S-form	1250:1257	arg1	SG					1259:1260	the 11S-form SG	1246:1260	the 11S-form SG at the quaternary level (into different [AB] subunits)	1246:1315	Dynamic latter scattering and spectroscopic results showed that the glycation resulted in a gradual dissociation of the 11S-form SG at the quaternary level (into different [AB] subunits), in a DG-dependent way, while their tertiary ([AB] subunits) and secondary structure were slightly affected.					
30372068	1	94	theme	plant	240:244	arg1	globulins					254:262	plant storage globulins	240:262	plant storage globulins	240:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30372068	11	95	with	glycation	2258:2266	arg1	polysaccharides					2273:2287	polysaccharides	2273:2287	polysaccharides	2273:2287	The findings would be of importance for providing new knowledge about the molecular mechanism for the modification of emulsifying properties of oligomeric globulins by the glycation with polysaccharides.					
30372068	10	96	theme	Pickering	2061:2069	arg1	stabilization					2071:2083	an outstanding Pickering stabilization	2046:2083	an outstanding Pickering stabilization	2046:2083	It was also confirmed that once the glycated SG adsorbed at the interface, it would readily dissociated into subunits; the dissociated [AB] subunits exhibited an outstanding Pickering stabilization.					
30372068	10	97	theme	dissociated	2010:2020	arg1	subunits					2027:2034	the dissociated [AB] subunits	2006:2034	the dissociated [AB] subunits	2006:2034	It was also confirmed that once the glycated SG adsorbed at the interface, it would readily dissociated into subunits; the dissociated [AB] subunits exhibited an outstanding Pickering stabilization.					
30372068	2	98	theme	soluble	370:376	arg1	SSPS					394:397	soy soluble polysaccharide (SSPS)	366:398	soy soluble polysaccharide (SSPS)	366:398	This work reported that the glycation with soy soluble polysaccharide (SSPS) progressively improved the emulsification efficiency of soy glycinin (SG) in a degree-of-glycation (DG)-dependent manner.					
30372068	1	99	theme	globulins	254:262	arg1	properties					226:235	the emulsifying properties	210:235	the emulsifying properties of plant storage globulins	210:262	Glycation with carbohydrates has been considered to be an effective strategy to improve the emulsifying properties of plant storage globulins, but the knowledge is inconsistent and even contradictory.					
30933786	0	0	theme	Lafont	68:73	arg1	rearrangement					75:87	the Lafont rearrangement	64:87	the Lafont rearrangement	64:87	Rapid synthesis of N-glycan oxazolines from locust bean gum via the Lafont rearrangement.					
30933786	1	1	theme	Manβ	142:145	arg1	disaccharide					157:168	a Manβ(1 → 4)Man disaccharide	140:168	a Manβ(1 → 4)Man disaccharide	140:168	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	1	2	theme	Manβ	208:211	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	1	3	theme	2-iodo-glycosyl	281:295	arg1	azide					297:301	a 2-iodo-glycosyl azide	279:301	a 2-iodo-glycosyl azide	279:301	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	1	4	theme	1 → 4	213:217	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	5	theme	ENGase	518:523	arg1	enzymes					525:531	ENGase enzymes	518:531	ENGase enzymes that is considerably shorter than those reported previously	518:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	1	6	theme	1 → 4	147:151	arg1	disaccharide					157:168	a Manβ(1 → 4)Man disaccharide	140:168	a Manβ(1 → 4)Man disaccharide	140:168	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	7	theme	N-glycan	433:440	arg1	structures					442:451	more complex N-glycan structures	420:451	more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	420:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	1	8	theme	Lafont	308:313	arg1	rearrangement					315:327	Lafont rearrangement	308:327	Lafont rearrangement	308:327	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	9	theme	Manβ	334:337	arg1	intermediate					386:397	a key intermediate	380:397	a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	380:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	9	theme	Manβ	334:337	arg1	disaccharide					352:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	1	10	theme	Man	153:155	arg1	disaccharide					157:168	a Manβ(1 → 4)Man disaccharide	140:168	a Manβ(1 → 4)Man disaccharide	140:168	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	0	11	theme	Rapid	0:4	arg1	synthesis					6:14	Rapid synthesis	0:14	Rapid synthesis of N-glycan	0:26	Rapid synthesis of N-glycan oxazolines from locust bean gum via the Lafont rearrangement.					
30933786	1	12	theme	GlcNAc	219:224	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	0	13	theme	N-glycan	19:26	arg1	synthesis					6:14	Rapid synthesis	0:14	Rapid synthesis of N-glycan	0:26	Rapid synthesis of N-glycan oxazolines from locust bean gum via the Lafont rearrangement.					
30933786	1	14	theme	disaccharide	226:237	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	1	15	theme	Enzymatic	90:98	arg1	degradation					100:110	Enzymatic degradation	90:110	Enzymatic degradation of locust bean gum	90:129	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	16	theme	N-glycan	474:481	arg1	oxazolines					483:492	N-glycan oxazolines	474:492	N-glycan oxazolines	474:492	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	17	theme	GlcNAc	345:350	arg1	intermediate					386:397	a key intermediate	380:397	a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	380:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	17	theme	GlcNAc	345:350	arg1	disaccharide					352:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	0	18	theme	bean	51:54	arg1	gum					56:58	locust bean gum	44:58	locust bean gum	44:58	Rapid synthesis of N-glycan oxazolines from locust bean gum via the Lafont rearrangement.					
30933786	1	19	theme	N-glycans	251:259	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	20	used	used	372:375	arg2	disaccharide					352:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	20	used	used	372:375	arg2	intermediate					386:397	a key intermediate	380:397	a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	380:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	0	21	theme	locust	44:49	arg1	gum					56:58	locust bean gum	44:58	locust bean gum	44:58	Rapid synthesis of N-glycan oxazolines from locust bean gum via the Lafont rearrangement.					
30933786	1	22	theme	locust	115:120	arg1	gum					127:129	locust bean gum	115:129	locust bean gum	115:129	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	23	theme	donor	497:501	arg1	route					465:469	a route	463:469	a route to N-glycan oxazolines	463:492	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	23	theme	donor	497:501	arg1	substrates					503:512	donor substrates	497:512	donor substrates for ENGase enzymes that is considerably shorter than those reported previously	497:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	1	24	theme	bean	122:125	arg1	gum					127:129	locust bean gum	115:129	locust bean gum	115:129	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	1	25	theme	gum	127:129	arg1	degradation					100:110	Enzymatic degradation	90:110	Enzymatic degradation of locust bean gum	90:129	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
30933786	2	26	theme	complex	425:431	arg1	structures					442:451	more complex N-glycan structures	420:451	more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	420:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	27	theme	key	382:384	arg1	intermediate					386:397	a key intermediate	380:397	a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously	380:591	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	2	27	theme	key	382:384	arg1	disaccharide					352:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide	330:363	The Manβ(1 → 4)GlcNAc disaccharide may be used as a key intermediate for elaboration into more complex N-glycan structures providing a route to N-glycan oxazolines as donor substrates for ENGase enzymes that is considerably shorter than those reported previously.					
30933786	1	28	theme	core	203:206	arg1	unit					239:242	the core Manβ(1 → 4)GlcNAc disaccharide unit	199:242	the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans	199:259	Enzymatic degradation of locust bean gum provides a Manβ(1 → 4)Man disaccharide, which may be converted into the core Manβ(1 → 4)GlcNAc disaccharide unit of all N-glycans via conversion to a 2-iodo-glycosyl azide, and Lafont rearrangement.					
29956878	3	0	theme	N-glycoproteins	333:347	arg1	bioactivities					306:318	bioactivities	306:318	bioactivities	306:318	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	3	0	theme	N-glycoproteins	333:347	arg1	properties					291:300	the intrinsic properties	277:300	the intrinsic properties	277:300	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	7	1	theme	resulting	738:746	arg1	structures					764:773	The resulting highly resolved structures	734:773	The resulting highly resolved structures	734:773	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	3	2	attach	linked	366:371	arg1	cancers					384:390	various cancers	376:390	various cancers	376:390	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	3	2	attach	linked	366:371	arg2	level					357:361	its level	353:361	its level	353:361	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	5	3	theme	protein	600:606	arg1	fold					608:611	a novel protein fold	592:611	a novel protein fold termed β-prism III	592:630	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	7	4	gly	α1,6-fucosylated	832:847	arg1	N-glycans					849:857	α1,6-fucosylated N-glycans	832:857	α1,6-fucosylated N-glycans	832:857	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	8	5	theme	consensus	925:933	arg1	motif					935:939	a protein consensus motif	915:939	a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding	915:1028	This work also provided a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding.					
29956878	3	6	theme	various	376:382	arg1	cancers					384:390	various cancers	376:390	various cancers	376:390	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	8	7	theme	N-glycan	996:1003	arg1	flexibility					1005:1015	N-glycan flexibility	996:1015	N-glycan flexibility	996:1015	This work also provided a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding.					
29956878	7	8	theme	α1,6-fucosylated	832:847	arg1	N-glycans					849:857	α1,6-fucosylated N-glycans	832:857	α1,6-fucosylated N-glycans	832:857	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	3	9	theme	Core	248:251	arg1	fucosylation					253:264	Core fucosylation	248:264	Core fucosylation	248:264	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	6	10	dep	12	692:693	arg1	to					689:690	to	689:690	to	689:690	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
29956878	6	11	theme	N-glycan	668:675	arg1	azides					677:682	Three biantennary core-fucosylated N-glycan azides	633:682	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars	633:700	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
29956878	6	12	gly	core-fucosylated	651:666	arg1	azides					677:682	Three biantennary core-fucosylated N-glycan azides	633:682	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars	633:700	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
29956878	1	13	theme	mini	72:75	arg1	PhoSL					91:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL was recombinantly produced and characterized.					
29956878	1	14	theme	fungal	77:82	arg1	PhoSL					91:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL was recombinantly produced and characterized.					
29956878	7	15	theme	small	869:873	arg1	protein					875:881	such a small protein	862:881	such a small protein	862:881	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	2	16	with	N-glycans	213:221	arg1	fucose					240:245	α1,6-linked fucose	228:245	α1,6-linked fucose	228:245	Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose.					
29956878	1	17	theme	lectin	84:89	arg1	PhoSL					91:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL	68:95	The mini fungal lectin PhoSL was recombinantly produced and characterized.					
29956878	6	18	theme	sugars	695:700	arg1	azides					677:682	Three biantennary core-fucosylated N-glycan azides	633:682	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars	633:700	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
29956878	0	19	theme	Core-Fucosylated	23:38	arg1	N-Glycans					40:48	Complex Core-Fucosylated N-Glycans	15:48	Complex Core-Fucosylated N-Glycans	15:48	Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.					
29956878	3	20	theme	intrinsic	281:289	arg1	properties					291:300	the intrinsic properties	277:300	the intrinsic properties	277:300	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	5	21	theme	novel	594:598	arg1	fold					608:611	a novel protein fold	592:611	a novel protein fold termed β-prism III	592:630	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	0	22	theme	Complex	15:21	arg1	N-Glycans					40:48	Complex Core-Fucosylated N-Glycans	15:48	Complex Core-Fucosylated N-Glycans	15:48	Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.					
29956878	2	23	theme	amino	171:175	arg1	acids					177:181	only 40 amino acids	163:181	only 40 amino acids	163:181	Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose.					
29956878	8	24	theme	protein	917:923	arg1	motif					935:939	a protein consensus motif	915:939	a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding	915:1028	This work also provided a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding.					
29956878	5	25	theme	crystal	487:493	arg1	structure					495:503	the crystal structure	483:503	the crystal structure	483:503	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	7	26	theme	exclusive	807:815	arg1	recognition					817:827	the exclusive recognition	803:827	the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein	803:881	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	2	27	theme	α1,6-linked	228:238	arg1	fucose					240:245	α1,6-linked fucose	228:245	α1,6-linked fucose	228:245	Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose.					
29956878	7	28	theme	N-glycans	849:857	arg1	recognition					817:827	the exclusive recognition	803:827	the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein	803:881	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	0	29	theme	N-Glycans	40:48	arg1	Recognition					0:10	Recognition	0:10	Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin	0:65	Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.					
29956878	7	30	theme	resolved	755:762	arg1	structures					764:773	The resulting highly resolved structures	734:773	The resulting highly resolved structures	734:773	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	0	31	theme	Mini	55:58	arg1	Lectin					60:65	a Mini Lectin	53:65	a Mini Lectin	53:65	Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.					
29956878	7	32	theme	detailed	782:789	arg1	view					791:794	a detailed view	780:794	a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs	780:888	The resulting highly resolved structures gave a detailed view on how the exclusive recognition of α1,6-fucosylated N-glycans by such a small protein occurs.					
29956878	3	33	theme	mammalian	323:331	arg1	N-glycoproteins					333:347	mammalian N-glycoproteins	323:347	mammalian N-glycoproteins	323:347	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	8	34	theme	observed	949:956	arg1	specificity					958:968	the observed specificity	945:968	the observed specificity	945:968	This work also provided a protein consensus motif for the observed specificity as well as a glimpse into N-glycan flexibility upon binding.					
29956878	4	35	theme	promising	417:425	arg1	tool					427:430	a promising tool	415:430	a promising tool for glycoprofiling	415:449	Thus, PhoSL serves as a promising tool for glycoprofiling.					
29956878	4	35	theme	promising	417:425	arg1	PhoSL					399:403	PhoSL	399:403	PhoSL	399:403	Thus, PhoSL serves as a promising tool for glycoprofiling.					
29956878	5	36	theme	interlaced	565:574	arg1	trimer					576:581	an interlaced trimer	562:581	an interlaced trimer creating a novel protein fold termed β-prism III	562:630	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	3	37	gly	N-glycoproteins	333:347	arg1	N-glycoproteins					333:347	mammalian N-glycoproteins	323:347	mammalian N-glycoproteins	323:347	Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers.					
29956878	5	38	theme	zinc	526:529	arg1	signal					541:546	the zinc anomalous signal	522:546	the zinc anomalous signal	522:546	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	5	39	theme	structural	460:469	arg1	precedence					471:480	structural precedence	460:480	structural precedence	460:480	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	2	40	theme	acids	177:181	arg1	length					153:158	a length	151:158	a length of only 40 amino acids	151:181	Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose.					
29956878	5	41	theme	anomalous	531:539	arg1	signal					541:546	the zinc anomalous signal	522:546	the zinc anomalous signal	522:546	Without structural precedence, the crystal structure was solved using the zinc anomalous signal, and revealed an interlaced trimer creating a novel protein fold termed β-prism III.					
29956878	6	42	theme	core-fucosylated	651:666	arg1	azides					677:682	Three biantennary core-fucosylated N-glycan azides	633:682	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars	633:700	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
29956878	2	43	link	α1,6-linked	228:238	arg1	fucose					240:245	α1,6-linked fucose	228:245	α1,6-linked fucose	228:245	Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose.					
29956878	6	44	theme	biantennary	639:649	arg1	azides					677:682	Three biantennary core-fucosylated N-glycan azides	633:682	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars	633:700	Three biantennary core-fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL.					
31382537	4	0	from	extracts	626:633	arg1	proteins					586:593	proteins	586:593	proteins from ectosomes, the whole cell extracts, and the membrane fractions	586:661	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	2	1	theme	melanoma	366:373	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	4	2	from	ectosomes	600:608	arg1	proteins					586:593	proteins	586:593	proteins from ectosomes, the whole cell extracts, and the membrane fractions	586:661	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	5	3	theme	altered	909:915	arg1	metabolism					917:926	altered metabolism	909:926	altered metabolism	909:926	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	7	4	theme	bisected	1154:1161	arg1	N-glycans					1176:1184	bisected complex type N-glycans	1154:1184	bisected complex type N-glycans	1154:1184	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	1	5	theme	extracellular	154:166	arg1	vesicles					168:175	extracellular vesicles	154:175	extracellular vesicles that often promote disease development and progression	154:230	Cancer cells are known to release extracellular vesicles that often promote disease development and progression.					
31382537	2	6	theme	uveal	360:364	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	5	7	theme	cancer	879:884	arg1	cell					886:889	cancer cell proliferation and altered metabolism	879:926	cancer cell proliferation and altered metabolism	879:926	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	4	8	theme	characteristic	751:764	arg1	structures					773:782	characteristic glycan structures	751:782	characteristic glycan structures	751:782	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	4	9	theme	whole	615:619	arg1	extracts					626:633	the whole cell extracts	611:633	the whole cell extracts	611:633	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	5	10	theme	tumor	929:933	arg1	invasion					935:942	tumor invasion	929:942	tumor invasion	929:942	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	6	11	link	Mel202-derived	1048:1061	arg1	ectosomes					1063:1071	Mel202-derived ectosomes	1048:1071	Mel202-derived ectosomes	1048:1071	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	7	12	theme	proteins	1140:1147	arg1	enrichment					1116:1125	Selective enrichment	1106:1125	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids	1106:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	4	13	theme	Western	705:711	arg1	blotting					713:720	Western blotting	705:720	Western blotting	705:720	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	0	14	theme	Biomarkers	108:117	arg1	Presence					77:84	the Presence	73:84	the Presence of Potentially Useful Biomarkers	73:117	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.					
31382537	3	15	theme	nano-LC-MS/MS	504:516	arg1	approach					518:525	a nano-LC-MS/MS approach	502:525	a nano-LC-MS/MS approach	502:525	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	8	16	theme	surface	1295:1301	arg1	glycosylation					1303:1315	the surface glycosylation	1291:1315	the surface glycosylation of Mel202 cells and ectosomes	1291:1345	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	17	dep	occurs	1402:1407	arg1	findings					1363:1370	recent findings	1356:1370	recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition	1356:1535	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	2	18	theme	glycosylation	288:300	arg1	pattern					302:308	glycosylation pattern	288:308	glycosylation pattern	288:308	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	6	19	theme	parental	1081:1088	arg1	membranes					1095:1103	the parental cell membranes	1077:1103	the parental cell membranes	1077:1103	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	3	20	theme	conditioned	447:457	arg1	media					459:463	conditioned media	447:463	conditioned media using sequential centrifugation	447:495	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	0	21	theme	Useful	101:106	arg1	Biomarkers					108:117	Potentially Useful Biomarkers	89:117	Potentially Useful Biomarkers	89:117	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.					
31382537	7	22	link	α2,6-linked	1190:1200	arg1	acids					1209:1213	α2,6-linked sialic acids	1190:1213	α2,6-linked sialic acids	1190:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	7	23	theme	complex	1163:1169	arg1	N-glycans					1176:1184	bisected complex type N-glycans	1154:1184	bisected complex type N-glycans	1154:1184	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	3	24	theme	protein	555:561	arg1	content					563:569	their protein content	549:569	their protein content	549:569	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	6	25	theme	cell	1090:1093	arg1	membranes					1095:1103	the parental cell membranes	1077:1103	the parental cell membranes	1077:1103	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	3	26	theme	Mel202	415:420	arg1	cells					422:426	Mel202 cells	415:426	Mel202 cells	415:426	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	2	27	dep	content	276:282	arg1	the					264:266	the	264:266	the	264:266	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	8	28	contain	contain	1489:1495	arg2	composition					1525:1535	glycan composition	1518:1535	glycan composition	1518:1535	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	28	contain	contain	1489:1495	arg1	ectosomes					1479:1487	ectosomes	1479:1487	ectosomes	1479:1487	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	28	contain	contain	1489:1495	arg2	protein					1506:1512	a unique protein	1497:1512	a unique protein	1497:1512	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	7	29	theme	Selective	1106:1114	arg1	enrichment					1116:1125	Selective enrichment	1106:1125	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids	1106:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	7	30	theme	α2,6-linked	1190:1200	arg1	acids					1209:1213	α2,6-linked sialic acids	1190:1213	α2,6-linked sialic acids	1190:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	8	31	theme	recent	1356:1361	arg1	findings					1363:1370	recent findings	1356:1370	recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition	1356:1535	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	3	32	attach	isolated	433:440	arg1	media					459:463	conditioned media	447:463	conditioned media using sequential centrifugation	447:495	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	3	32	attach	isolated	433:440	arg2	Ectosomes					393:401	Ectosomes	393:401	Ectosomes released by Mel202 cells	393:426	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	4	33	theme	glycan	766:771	arg1	structures					773:782	characteristic glycan structures	751:782	characteristic glycan structures	751:782	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	4	34	from	fractions	653:661	arg1	proteins					586:593	proteins	586:593	proteins from ectosomes, the whole cell extracts, and the membrane fractions	586:661	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	1	35	theme	disease	196:202	arg1	development					204:214	disease development	196:214	disease development	196:214	Cancer cells are known to release extracellular vesicles that often promote disease development and progression.					
31382537	5	36	dep	cell	886:889	arg1	proliferation					891:903	proliferation	891:903	proliferation	891:903	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	5	36	dep	cell	886:889	arg1	metabolism					917:926	altered metabolism	909:926	altered metabolism	909:926	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	5	37	theme	drug	960:963	arg1	resistance					965:974	drug resistance	960:974	drug resistance	960:974	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	8	38	theme	unique	1499:1504	arg1	protein					1506:1512	a unique protein	1497:1512	a unique protein	1497:1512	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	2	39	theme	ectosomes	313:321	arg1	pattern					302:308	glycosylation pattern	288:308	glycosylation pattern	288:308	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	2	39	theme	ectosomes	313:321	arg1	content					276:282	protein content	268:282	protein content	268:282	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	8	40	theme	determined	1425:1434	arg1	fragments					1436:1444	strictly determined fragments	1416:1444	strictly determined fragments of the plasma membrane	1416:1467	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	4	41	theme	lectins	691:697	arg1	panel					682:686	a panel	680:686	a panel of lectins	680:697	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	2	42	theme	human	346:350	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	6	43	theme	Mel202-derived	1048:1061	arg1	ectosomes					1063:1071	Mel202-derived ectosomes	1048:1071	Mel202-derived ectosomes	1048:1071	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	8	44	theme	plasma	1453:1458	arg1	membrane					1460:1467	the plasma membrane	1449:1467	the plasma membrane	1449:1467	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	5	45	theme	them	846:849	arg1	them					846:849	them	846:849	them	846:849	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	5	45	theme	them	846:849	arg1	many					838:841	many	838:841	many	838:841	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	8	46	theme	membrane	1460:1467	arg1	fragments					1436:1444	strictly determined fragments	1416:1444	strictly determined fragments of the plasma membrane	1416:1467	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	47	theme	cells	1327:1331	arg1	glycosylation					1303:1315	the surface glycosylation	1291:1315	the surface glycosylation of Mel202 cells and ectosomes	1291:1345	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	2	48	theme	present	237:243	arg1	study					245:249	The present study	233:249	The present study	233:249	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	2	49	gly	glycosylation	288:300	arg1	ectosomes					313:321	ectosomes	313:321	ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line	313:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	0	50	theme	Melanoma-Derived	38:53	arg1	Ectosomes					55:63	Uveal Melanoma-Derived Ectosomes	32:63	Uveal Melanoma-Derived Ectosomes	32:63	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.					
31382537	8	51	theme	ectosomes	1392:1400	arg1	budding					1381:1387	the budding	1377:1387	the budding of ectosomes	1377:1400	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	2	52	theme	primary	352:358	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	0	53	theme	Uveal	32:36	arg1	Ectosomes					55:63	Uveal Melanoma-Derived Ectosomes	32:63	Uveal Melanoma-Derived Ectosomes	32:63	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.					
31382537	8	54	gly	glycosylation	1303:1315	arg1	ectosomes					1337:1345	ectosomes	1337:1345	ectosomes	1337:1345	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	54	gly	glycosylation	1303:1315	arg1	cells					1327:1331	Mel202 cells	1320:1331	Mel202 cells	1320:1331	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	55	theme	glycan	1518:1523	arg1	composition					1525:1535	glycan composition	1518:1535	glycan composition	1518:1535	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	1	56	theme	Cancer	120:125	arg1	cells					127:131	Cancer cells	120:131	Cancer cells	120:131	Cancer cells are known to release extracellular vesicles that often promote disease development and progression.					
31382537	7	57	theme	type	1171:1174	arg1	N-glycans					1176:1184	bisected complex type N-glycans	1154:1184	bisected complex type N-glycans	1154:1184	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	5	58	theme	unique	801:806	arg1	proteins					808:815	As many as 2527 unique proteins	785:815	As many as 2527 unique proteins	785:815	As many as 2527 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis, or drug resistance.					
31382537	3	59	used	used	531:534	arg2	approach					518:525	a nano-LC-MS/MS approach	502:525	a nano-LC-MS/MS approach	502:525	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	6	60	theme	glycosylation	1018:1030	arg1	pattern					1032:1038	a distinct glycosylation pattern	1007:1038	a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes	1007:1103	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	7	61	with	enrichment	1116:1125	arg1	N-glycans					1176:1184	bisected complex type N-glycans	1154:1184	bisected complex type N-glycans	1154:1184	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	7	61	with	enrichment	1116:1125	arg1	acids					1209:1213	α2,6-linked sialic acids	1190:1213	α2,6-linked sialic acids	1190:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	6	62	theme	Lectin-based	977:988	arg1	studies					990:996	Lectin-based studies	977:996	Lectin-based studies	977:996	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	8	63	from	Differences	1276:1286	arg1	glycosylation					1303:1315	the surface glycosylation	1291:1315	the surface glycosylation of Mel202 cells and ectosomes	1291:1345	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	8	64	theme	ectosomes	1337:1345	arg1	glycosylation					1303:1315	the surface glycosylation	1291:1315	the surface glycosylation of Mel202 cells and ectosomes	1291:1345	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	2	65	theme	protein	268:274	arg1	content					276:282	protein content	268:282	protein content	268:282	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	7	66	theme	ectosomal	1130:1138	arg1	proteins					1140:1147	ectosomal proteins	1130:1147	ectosomal proteins	1130:1147	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	4	67	theme	flow	726:729	arg1	cytometry					731:739	flow cytometry	726:739	flow cytometry	726:739	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	4	68	theme	cell	621:624	arg1	extracts					626:633	the whole cell extracts	611:633	the whole cell extracts	611:633	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	3	69	theme	sequential	471:480	arg1	centrifugation					482:495	sequential centrifugation	471:495	sequential centrifugation	471:495	Ectosomes released by Mel202 cells were isolated from conditioned media using sequential centrifugation, and a nano-LC-MS/MS approach was used to determine their protein content.					
31382537	8	70	theme	Mel202	1320:1325	arg1	cells					1327:1331	Mel202 cells	1320:1331	Mel202 cells	1320:1331	Differences in the surface glycosylation of Mel202 cells and ectosomes supports recent findings that the budding of ectosomes occurs within strictly determined fragments of the plasma membrane, and thus ectosomes contain a unique protein and glycan composition.					
31382537	4	71	theme	membrane	644:651	arg1	fractions					653:661	the membrane fractions	640:661	the membrane fractions	640:661	Subsequently, proteins from ectosomes, the whole cell extracts, and the membrane fractions were probed with a panel of lectins using Western blotting and flow cytometry to reveal characteristic glycan structures.					
31382537	2	72	theme	cell	382:385	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	6	73	theme	distinct	1009:1016	arg1	pattern					1032:1038	a distinct glycosylation pattern	1007:1038	a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes	1007:1103	Lectin-based studies revealed a distinct glycosylation pattern between Mel202-derived ectosomes and the parental cell membranes.					
31382537	0	74	theme	Ectosomes	55:63	arg1	Proteome					20:27	the Proteome	16:27	the Proteome of Uveal Melanoma-Derived Ectosomes	16:63	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.					
31382537	7	75	theme	sialic	1202:1207	arg1	acids					1209:1213	α2,6-linked sialic acids	1190:1213	α2,6-linked sialic acids	1190:1213	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31382537	2	76	theme	Mel202	375:380	arg1	line					387:390	a human primary uveal melanoma Mel202 cell line	344:390	a human primary uveal melanoma Mel202 cell line	344:390	The present study investigated the protein content and glycosylation pattern of ectosomes released in vitro by a human primary uveal melanoma Mel202 cell line.					
31382537	7	77	theme	ectosome	1238:1245	arg1	formation					1247:1255	ectosome formation	1238:1255	ectosome formation	1238:1255	Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acids may be significant for ectosome formation and sequestration.					
31298840	4	0	theme	graphitized	582:592	arg1	gold-standard					676:688	the gold-standard	672:688	the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	672:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	0	theme	graphitized	582:592	arg1	chromatography					608:621	Porous graphitized carbon liquid chromatography	575:621	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS)	575:658	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	1	1	from	functions	206:214	arg1	surfaces					228:235	cellular surfaces	219:235	cellular surfaces	219:235	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	2	2	theme	competitive	395:405	arg1	glycosyltransferases/glycosidases					407:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and competitive glycosyltransferases/glycosidases.					
31298840	6	3	theme	oligosaccharides	1119:1134	arg1	separation					1096:1105	gas-phase separation	1086:1105	gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans	1086:1203	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	7	4	theme	IM-	1250:1252	arg1	comparison					1232:1241	a direct comparison	1223:1241	a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins	1223:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	6	5	theme	isomeric	1110:1117	arg1	oligosaccharides					1119:1134	isomeric oligosaccharides	1110:1134	isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans	1110:1203	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	4	6	theme	liquid	601:606	arg1	gold-standard					676:688	the gold-standard	672:688	the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	672:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	6	theme	liquid	601:606	arg1	chromatography					608:621	Porous graphitized carbon liquid chromatography	575:621	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS)	575:658	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	7	7	theme	salivary	1316:1323	arg1	mucins					1325:1330	porcine gastric and human salivary mucins	1290:1330	mucins	1325:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	6	8	theme	mass	1023:1026	arg1	IM-MS					1042:1046	IM-MS	1042:1046	IM-MS	1042:1046	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	6	8	theme	mass	1023:1026	arg1	spectrometry					1028:1039	ion mobility mass spectrometry	1010:1039	ion mobility mass spectrometry (IM-MS)	1010:1047	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	8	9	theme	methods	1443:1449	arg1	combination					1420:1430	the combination	1416:1430	the combination of the two methods	1416:1449	Our results identify structures, which are resolved by PGCLC and/or IM, validating the combination of the two methods.					
31298840	4	10	from	samples	796:802	arg1	typical					774:780	typical	774:780	typical	774:780	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	3	11	theme	characterization	557:572	arg1	terms					521:525	terms	521:525	terms of sensitivity and structural characterization	521:572	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	5	12	theme	chromatographic	851:865	arg1	separation					867:876	chromatographic separation	851:876	chromatographic separation of isomers	851:887	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	6	13	theme	mobility	1014:1021	arg1	IM-MS					1042:1046	IM-MS	1042:1046	IM-MS	1042:1046	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	6	13	theme	mobility	1014:1021	arg1	spectrometry					1028:1039	ion mobility mass spectrometry	1010:1039	ion mobility mass spectrometry (IM-MS)	1010:1047	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	1	14	theme	biological	195:204	arg1	functions					206:214	biological functions	195:214	biological functions on cellular surfaces and within cells	195:252	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	5	15	from	due	830:832	arg1	part					843:846	large part	837:846	large part	837:846	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	4	16	theme	Porous	575:580	arg1	gold-standard					676:688	the gold-standard	672:688	the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	672:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	16	theme	Porous	575:580	arg1	chromatography					608:621	Porous graphitized carbon liquid chromatography	575:621	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS)	575:658	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	7	17	theme	direct	1225:1230	arg1	comparison					1232:1241	a direct comparison	1223:1241	a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins	1223:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	4	18	theme	structural	698:707	arg1	characterization					709:724	the structural characterization	694:724	the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	694:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	19	theme	typical	774:780	arg1	mixtures					765:772	complex mixtures	757:772	especially complex mixtures typical in biological samples	746:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	19	theme	typical	774:780	arg1	glycoconjugates					729:743	glycoconjugates	729:743	glycoconjugates	729:743	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	3	20	theme	structural	449:458	arg1	viewpoint					460:468	a structural viewpoint	447:468	a structural viewpoint	447:468	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	4	21	from	typical	774:780	arg1	samples					796:802	biological samples	785:802	biological samples	785:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	7	22	from	mucins	1325:1330	arg1	LC-separation					1258:1270	LC-separation	1258:1270	LC-separation	1258:1270	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	22	from	mucins	1325:1330	arg1	comparison					1232:1241	a direct comparison	1223:1241	a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins	1223:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	22	from	mucins	1325:1330	arg1	IM-					1250:1252	IM-	1250:1252	IM-	1250:1252	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	6	23	theme	gas-phase	1086:1094	arg1	separation					1096:1105	gas-phase separation	1086:1105	gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans	1086:1203	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	3	24	theme	particular	497:506	arg1	challenge					508:516	a particular challenge	495:516	a particular challenge in terms of sensitivity and structural characterization	495:572	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	0	25	theme	O-Glycans	23:31	arg1	Separation					0:9	Separation	0:9	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.	0:92	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.					
31298840	3	26	theme	sensitivity	530:540	arg1	terms					521:525	terms	521:525	terms of sensitivity and structural characterization	521:572	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	0	27	theme	Isomeric	14:21	arg1	O-Glycans					23:31	Isomeric O-Glycans	14:31	Isomeric O-Glycans	14:31	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.					
31298840	9	28	theme	samples	1615:1621	arg1	glycome					1596:1602	the full glycome	1587:1602	the full glycome of unknown samples	1587:1621	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
31298840	0	29	theme	Ion	36:38	arg1	Mobility					40:47	Ion Mobility	36:47	Ion Mobility	36:47	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.					
31298840	6	30	theme	effective	1067:1075	arg1	tool					1077:1080	an effective tool	1064:1080	an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans	1064:1203	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	7	31	theme	human	1310:1314	arg1	mucins					1325:1330	porcine gastric and human salivary mucins	1290:1330	mucins	1325:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	9	32	theme	full	1591:1594	arg1	glycome					1596:1602	the full glycome	1587:1602	the full glycome of unknown samples	1587:1621	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
31298840	1	33	theme	cellular	219:226	arg1	surfaces					228:235	cellular surfaces	219:235	cellular surfaces	219:235	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	6	34	theme	small	1168:1172	arg1	oligosaccharides					1174:1189	small oligosaccharides	1168:1189	small oligosaccharides	1168:1189	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	7	35	theme	O-glycans	1275:1283	arg1	LC-separation					1258:1270	LC-separation	1258:1270	LC-separation	1258:1270	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	35	theme	O-glycans	1275:1283	arg1	IM-					1250:1252	IM-	1250:1252	IM-	1250:1252	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	4	36	theme	mass	631:634	arg1	spectrometry					636:647	mass spectrometry	631:647	mass spectrometry (PGCLC-MS)	631:658	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	36	theme	mass	631:634	arg1	PGCLC-MS					650:657	PGCLC-MS	650:657	PGCLC-MS	650:657	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	5	37	theme	large	837:841	arg1	part					843:846	large part	837:846	large part	837:846	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	5	38	from	part	843:846	arg1	due					830:832	due	830:832	due	830:832	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	4	39	theme	complex	757:763	arg1	mixtures					765:772	complex mixtures	757:772	especially complex mixtures typical in biological samples	746:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	39	theme	complex	757:763	arg1	glycoconjugates					729:743	glycoconjugates	729:743	glycoconjugates	729:743	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	7	40	from	comparison	1232:1241	arg1	mucins					1325:1330	porcine gastric and human salivary mucins	1290:1330	mucins	1325:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	40	from	comparison	1232:1241	arg1	gastric					1298:1304	gastric	1298:1304	gastric	1298:1304	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	5	41	theme	isomers	881:887	arg1	separation					867:876	chromatographic separation	851:876	chromatographic separation of isomers	851:887	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	2	42	theme	Glycan	255:260	arg1	structures					262:271	Glycan structures	255:271	Glycan structures	255:271	Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and competitive glycosyltransferases/glycosidases.					
31298840	6	43	theme	ion	1010:1012	arg1	IM-MS					1042:1046	IM-MS	1042:1046	IM-MS	1042:1046	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	6	43	theme	ion	1010:1012	arg1	spectrometry					1028:1039	ion mobility mass spectrometry	1010:1039	ion mobility mass spectrometry (IM-MS)	1010:1047	More recently, ion mobility mass spectrometry (IM-MS) has emerged as an effective tool for gas-phase separation of isomeric oligosaccharides that has been demonstrated with small oligosaccharides and N-glycans.					
31298840	2	44	theme	sequential	380:389	arg1	glycosyltransferases/glycosidases					407:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and competitive glycosyltransferases/glycosidases.					
31298840	7	45	theme	LC-separation	1258:1270	arg1	comparison					1232:1241	a direct comparison	1223:1241	a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins	1223:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	9	46	theme	unknown	1607:1613	arg1	samples					1615:1621	unknown samples	1607:1621	unknown samples	1607:1621	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
31298840	2	47	theme	multiple	371:378	arg1	glycosyltransferases/glycosidases					407:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	multiple sequential and competitive glycosyltransferases/glycosidases	371:439	Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and competitive glycosyltransferases/glycosidases.					
31298840	5	48	theme	high	810:813	arg1	performance					815:825	This high performance	805:825	This high performance	805:825	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	4	49	theme	biological	785:794	arg1	samples					796:802	biological samples	785:802	biological samples	785:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	1	50	theme	important	127:135	arg1	modifications					156:168	the most important post-translational modifications	118:168	the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells	118:252	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	2	51	from	genome	302:307	arg1	predictable					281:291	predictable	281:291	predictable	281:291	Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and competitive glycosyltransferases/glycosidases.					
31298840	9	52	theme	techniques	1494:1503	arg1	incorporation					1472:1484	the incorporation	1468:1484	the incorporation of both techniques into a single platform	1468:1526	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
31298840	9	52	theme	techniques	1494:1503	arg1	powerful					1537:1544	powerful	1537:1544	powerful	1537:1544	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
31298840	7	53	from	gastric	1298:1304	arg1	LC-separation					1258:1270	LC-separation	1258:1270	LC-separation	1258:1270	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	53	from	gastric	1298:1304	arg1	comparison					1232:1241	a direct comparison	1223:1241	a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins	1223:1330	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	7	53	from	gastric	1298:1304	arg1	IM-					1250:1252	IM-	1250:1252	IM-	1250:1252	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	1	54	theme	post-translational	137:154	arg1	modifications					156:168	the most important post-translational modifications	118:168	the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells	118:252	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	3	55	theme	structural	546:555	arg1	characterization					557:572	structural characterization	546:572	structural characterization	546:572	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	0	56	theme	Chromatography-Mass	60:78	arg1	Spectrometry					80:91	Liquid Chromatography-Mass Spectrometry	53:91	Liquid Chromatography-Mass Spectrometry	53:91	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.					
31298840	5	57	theme	mass	976:979	arg1	spectrometer					981:992	the mass spectrometer	972:992	the mass spectrometer	972:992	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	5	58	theme	glycan	962:967	arg1	fragmentation					940:952	fragmentation	940:952	fragmentation of each glycan	940:967	This high performance is due in large part to chromatographic separation of isomers and the information delivered by collision induced fragmentation of each glycan in the mass spectrometer.					
31298840	1	59	theme	modifications	156:168	arg1	one					111:113	one	111:113	one	111:113	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	1	59	theme	modifications	156:168	arg1	modifications					156:168	the most important post-translational modifications	118:168	the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells	118:252	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	4	60	theme	glycoconjugates	729:743	arg1	characterization					709:724	the structural characterization	694:724	the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	694:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	0	61	theme	Liquid	53:58	arg1	Spectrometry					80:91	Liquid Chromatography-Mass Spectrometry	53:91	Liquid Chromatography-Mass Spectrometry	53:91	Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry.					
31298840	4	62	theme	carbon	594:599	arg1	gold-standard					676:688	the gold-standard	672:688	the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples	672:802	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	4	62	theme	carbon	594:599	arg1	chromatography					608:621	Porous graphitized carbon liquid chromatography	575:621	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS)	575:658	Porous graphitized carbon liquid chromatography coupled mass spectrometry (PGCLC-MS) is arguably the gold-standard for the structural characterization of glycoconjugates, especially complex mixtures typical in biological samples.					
31298840	7	63	dep	IM-	1250:1252	arg1	the					1246:1248	the	1246:1248	the	1246:1248	Here, we present a direct comparison of the IM- and LC-separation of O-glycans from porcine gastric and human salivary mucins.					
31298840	3	64	from	challenge	508:516	arg1	terms					521:525	terms	521:525	terms of sensitivity and structural characterization	521:572	From a structural viewpoint, their analysis presents a particular challenge in terms of sensitivity and structural characterization.					
31298840	1	65	theme	essential	170:178	arg1	modifications					156:168	the most important post-translational modifications	118:168	the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells	118:252	Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells.					
31298840	9	66	theme	single	1512:1517	arg1	platform					1519:1526	a single platform	1510:1526	a single platform	1510:1526	Taken together, the incorporation of both techniques into a single platform would be powerful and undoubtedly valuable for determining the full glycome of unknown samples.					
30052167	3	0	theme	taxonomic	329:337	arg1	distribution					339:350	taxonomic distribution	329:350	taxonomic distribution	329:350	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	6	1	from	similarity	1090:1099	arg1	regulation					1109:1118	gene regulation	1104:1118	gene regulation	1104:1118	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	6	1	from	similarity	1090:1099	arg1	activity					1077:1084	metabolic activity	1067:1084	metabolic activity	1067:1084	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	5	2	theme	trehalase	950:958	arg1	neighbourhood					960:972	trehalase neighbourhood	950:972	trehalase neighbourhood	950:972	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	10	3	theme	diverse	1593:1599	arg1	species					1601:1607	diverse species	1593:1607	diverse species	1593:1607	This study serves as an instigation to understand evolution and functionality of trehalase across diverse species.					
30052167	0	4	theme	trehalases	102:111	arg1	diversification					56:70	diversification	56:70	diversification of prokaryotic and eukaryotic trehalases	56:111	Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases.					
30052167	4	5	theme	glycosyl	552:559	arg1	family					571:576	glycosyl hydrolase family 37	552:579	glycosyl hydrolase family 37	552:579	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	9	6	with	comparison	1410:1419	arg1	trehalase					1431:1439	acid trehalase	1426:1439	acid trehalase	1426:1439	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	6	7	theme	metabolic	1067:1075	arg1	activity					1077:1084	metabolic activity	1067:1084	metabolic activity	1067:1084	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	4	8	from	present	584:590	arg1	most					595:598	most	595:598	most	595:598	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	4	8	from	present	584:590	arg1	sequences					607:615	the sequences	603:615	the sequences	603:615	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	10	9	theme	trehalase	1576:1584	arg1	evolution					1545:1553	evolution	1545:1553	evolution	1545:1553	This study serves as an instigation to understand evolution and functionality of trehalase across diverse species.					
30052167	10	9	theme	trehalase	1576:1584	arg1	functionality					1559:1571	functionality	1559:1571	functionality	1559:1571	This study serves as an instigation to understand evolution and functionality of trehalase across diverse species.					
30052167	2	10	theme	major	305:309	arg1	species					311:317	major species	305:317	major species	305:317	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	3	11	theme	functional	378:387	arg1	domains					389:395	functional domains	378:395	functional domains	378:395	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	5	12	dep	transport	992:1000	arg1	genes					1023:1027	genes	1023:1027	genes	1023:1027	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	8	13	theme	residues	1397:1404	arg1	conservation					1358:1369	conservation	1358:1369	conservation of functionally important residues	1358:1404	Moreover, site-specific rate of evolution emphasized conservation of functionally important residues.					
30052167	9	14	theme	neutral	1442:1448	arg1	trehalase					1450:1458	neutral trehalase	1442:1458	neutral trehalase	1442:1458	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	8	15	theme	important	1387:1395	arg1	residues					1397:1404	functionally important residues	1374:1404	functionally important residues	1374:1404	Moreover, site-specific rate of evolution emphasized conservation of functionally important residues.					
30052167	2	16	theme	trehalase	292:300	arg1	origin					268:273	enigmatic origin	258:273	enigmatic origin	258:273	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	2	16	theme	trehalase	292:300	arg1	evolution					279:287	evolution	279:287	evolution	279:287	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	5	17	theme	sugar	986:990	arg1	transport					992:1000	sugar transport	986:1000	sugar transport	986:1000	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	2	18	dep	origin	268:273	arg1	the					254:256	the	254:256	the	254:256	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	3	19	theme	phylogeny	364:372	arg1	Study					320:324	Study	320:324	Study of taxonomic distribution, orthology, phylogeny and functional domains	320:395	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	4	20	theme	Domain	524:529	arg1	analysis					531:538	Domain analysis	524:538	Domain analysis	524:538	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	6	21	from	relatedness	1052:1062	arg1	regulation					1109:1118	gene regulation	1104:1118	gene regulation	1104:1118	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	6	21	from	relatedness	1052:1062	arg1	activity					1077:1084	metabolic activity	1067:1084	metabolic activity	1067:1084	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	4	22	from	most	595:598	arg1	present					584:590	present	584:590	present	584:590	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	5	23	theme	Gene	915:918	arg1	analysis					928:935	Gene context analysis	915:935	Gene context analysis	915:935	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	7	24	theme	horizontal	1279:1288	arg1	transfer					1295:1302	horizontal gene transfer	1279:1302	horizontal gene transfer	1279:1302	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	3	25	theme	distribution	339:350	arg1	Study					320:324	Study	320:324	Study of taxonomic distribution, orthology, phylogeny and functional domains	320:395	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	5	26	theme	context	920:926	arg1	analysis					928:935	Gene context analysis	915:935	Gene context analysis	915:935	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	0	27	theme	Evolutionary	0:11	arg1	analysis					36:43	Evolutionary and structure-function analysis	0:43	analysis	36:43	Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases.					
30052167	5	28	theme	lipid	1006:1010	arg1	metabolism					1012:1021	lipid metabolism	1006:1021	lipid metabolism	1006:1021	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	2	29	from	origin	268:273	arg1	species					311:317	major species	305:317	major species	305:317	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	4	30	theme	cytosolic	696:704	arg1	trehalase					706:714	cytosolic trehalase	696:714	cytosolic trehalase	696:714	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	3	31	theme	domains	389:395	arg1	Study					320:324	Study	320:324	Study of taxonomic distribution, orthology, phylogeny and functional domains	320:395	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	9	32	theme	extra	1467:1471	arg1	extension					1484:1492	an extra N-terminal extension	1464:1492	an extra N-terminal extension	1464:1492	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	7	33	theme	selection	1152:1160	arg1	analysis					1171:1178	Evolutionary and selection pressure analysis	1135:1178	analysis	1171:1178	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	5	34	theme	environmental	874:886	arg1	conditions					888:897	adverse environmental conditions	866:897	adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes	866:1027	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	0	35	theme	structure-function	17:34	arg1	analysis					36:43	Evolutionary and structure-function analysis	0:43	analysis	36:43	Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases.					
30052167	9	36	theme	N-terminal	1473:1482	arg1	extension					1484:1492	an extra N-terminal extension	1464:1492	an extra N-terminal extension	1464:1492	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	7	37	theme	pressure	1162:1169	arg1	analysis					1171:1178	Evolutionary and selection pressure analysis	1135:1178	analysis	1171:1178	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	2	38	from	evolution	279:287	arg1	species					311:317	major species	305:317	major species	305:317	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	3	39	theme	horizontal	498:507	arg1	transfer					514:521	horizontal gene transfer	498:521	horizontal gene transfer	498:521	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	5	40	theme	domain	815:820	arg1	rearrangement					822:834	domain rearrangement	815:834	domain rearrangement	815:834	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	4	41	theme	transmembrane	738:750	arg1	form					752:755	its transmembrane form	734:755	its transmembrane form	734:755	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	7	42	theme	Evolutionary	1135:1146	arg1	analysis					1171:1178	Evolutionary and selection pressure analysis	1135:1178	analysis	1171:1178	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	7	43	theme	gene	1290:1293	arg1	transfer					1295:1302	horizontal gene transfer	1279:1302	horizontal gene transfer	1279:1302	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	3	44	theme	other	479:483	arg1	taxa					485:488	other taxa	479:488	other taxa	479:488	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	8	45	theme	site-specific	1315:1327	arg1	rate					1329:1332	site-specific rate	1315:1332	site-specific rate of evolution	1315:1345	Moreover, site-specific rate of evolution emphasized conservation of functionally important residues.					
30052167	11	46	theme	Ramaswamy	1626:1634	arg1	Sarma					1639:1643	Ramaswamy H. Sarma	1626:1643	Ramaswamy H. Sarma	1626:1643	Communicated by Ramaswamy H. Sarma.					
30052167	0	47	theme	prokaryotic	75:85	arg1	trehalases					102:111	prokaryotic and eukaryotic trehalases	75:111	prokaryotic and eukaryotic trehalases	75:111	Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases.					
30052167	4	48	theme	hydrolase	561:569	arg1	family					571:576	glycosyl hydrolase family 37	552:579	glycosyl hydrolase family 37	552:579	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	5	49	theme	adverse	866:872	arg1	conditions					888:897	adverse environmental conditions	866:897	adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes	866:1027	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	9	50	contain	has	1460:1462	arg2	extension					1484:1492	an extra N-terminal extension	1464:1492	an extra N-terminal extension	1464:1492	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	9	50	contain	has	1460:1462	arg1	trehalase					1450:1458	neutral trehalase	1442:1458	neutral trehalase	1442:1458	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	5	51	dep	pH.	911:913	arg1	depicts					937:943	depicts	937:943	depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes	937:1027	Furthermore, it was observed that trehalase went through domain rearrangement to facilitate its activity in adverse environmental conditions like acidic pH. Gene context analysis depicts that trehalase neighbourhood consists of sugar transport and lipid metabolism genes.					
30052167	7	52	theme	trehalase	1198:1206	arg1	genes					1208:1212	trehalase genes	1198:1212	trehalase genes	1198:1212	Evolutionary and selection pressure analysis demonstrated that trehalase genes were duplicated and evolved under purifying selection, following horizontal gene transfer.					
30052167	1	53	theme	trehalose	151:159	arg1	breakdown					138:146	the breakdown	134:146	the breakdown of trehalose into two glucose moieties	134:185	Trehalase catalyses the breakdown of trehalose into two glucose moieties and is ubiquitous in all organisms.					
30052167	4	54	located	present	584:590	arg1	most					595:598	most	595:598	most	595:598	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	4	54	located	present	584:590	arg1	sequences					607:615	the sequences	603:615	the sequences	603:615	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	4	54	located	present	584:590	arg2	family					571:576	glycosyl hydrolase family 37	552:579	glycosyl hydrolase family 37	552:579	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	3	55	theme	gene	509:512	arg1	transfer					514:521	horizontal gene transfer	498:521	horizontal gene transfer	498:521	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	6	56	theme	gene	1104:1107	arg1	regulation					1109:1118	gene regulation	1104:1118	gene regulation	1104:1118	This highlights their relatedness in metabolic activity and similarity in gene regulation, respectively.					
30052167	4	57	attach	present	584:590	arg1	most					595:598	most	595:598	most	595:598	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	4	57	attach	present	584:590	arg1	sequences					607:615	the sequences	603:615	the sequences	603:615	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	4	57	attach	present	584:590	arg2	family					571:576	glycosyl hydrolase family 37	552:579	glycosyl hydrolase family 37	552:579	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	8	58	theme	evolution	1337:1345	arg1	rate					1329:1332	site-specific rate	1315:1332	site-specific rate of evolution	1315:1345	Moreover, site-specific rate of evolution emphasized conservation of functionally important residues.					
30052167	4	59	theme	dominant	632:639	arg1	activity					641:648	dominant activity	632:648	dominant activity	632:648	Domain analysis showed that glycosyl hydrolase family 37 is present in most of the sequences and represents dominant activity during evolution, and also, illustrating that cytosolic trehalase is primitive than its transmembrane form.					
30052167	0	60	theme	eukaryotic	91:100	arg1	trehalases					102:111	prokaryotic and eukaryotic trehalases	75:111	prokaryotic and eukaryotic trehalases	75:111	Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases.					
30052167	1	61	from	organisms	212:220	arg1	ubiquitous					194:203	ubiquitous	194:203	ubiquitous	194:203	Trehalase catalyses the breakdown of trehalose into two glucose moieties and is ubiquitous in all organisms.					
30052167	1	62	theme	glucose	170:176	arg1	moieties					178:185	two glucose moieties	166:185	two glucose moieties	166:185	Trehalase catalyses the breakdown of trehalose into two glucose moieties and is ubiquitous in all organisms.					
30052167	11	63	theme	H.	1636:1637	arg1	Sarma					1639:1643	Ramaswamy H. Sarma	1626:1643	Ramaswamy H. Sarma	1626:1643	Communicated by Ramaswamy H. Sarma.					
30052167	3	64	theme	orthology	353:361	arg1	Study					320:324	Study	320:324	Study of taxonomic distribution, orthology, phylogeny and functional domains	320:395	Study of taxonomic distribution, orthology, phylogeny and functional domains indicated that trehalase possibly originates from bacteria and was transmitted to other taxa through horizontal gene transfer.					
30052167	9	65	theme	acid	1426:1429	arg1	trehalase					1431:1439	acid trehalase	1426:1439	acid trehalase	1426:1439	In comparison with acid trehalase, neutral trehalase has an extra N-terminal extension.					
30052167	2	66	theme	enigmatic	258:266	arg1	origin					268:273	enigmatic origin	258:273	enigmatic origin	258:273	Here, we provide insights into the enigmatic origin and evolution of trehalase in major species.					
30052167	1	67	from	ubiquitous	194:203	arg1	organisms					212:220	all organisms	208:220	all organisms	208:220	Trehalase catalyses the breakdown of trehalose into two glucose moieties and is ubiquitous in all organisms.					
31430121	6	0	theme	substrates	1368:1377	arg1	exception					1355:1363	the exception	1351:1363	the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product	1351:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	4	1	theme	acetylation	957:967	arg1	patterns					969:976	defined acetylation patterns	949:976	defined acetylation patterns	949:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	7	2	theme	specific	1590:1597	arg1	glycosidase					1599:1609	dPNAG specific glycosidase	1584:1609	dPNAG specific glycosidase	1584:1609	These observations provide critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts.					
31430121	2	3	theme	dPNAG	407:411	arg1	function					395:402	function	395:402	function	395:402	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	2	3	theme	dPNAG	407:411	arg1	assembly					382:389	proper assembly	375:389	proper assembly	375:389	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	4	4	with	analogs	936:942	arg1	patterns					969:976	defined acetylation patterns	949:976	defined acetylation patterns	949:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	5	5	theme	DspB	1013:1016	arg1	substrates					1018:1027	effective DspB substrates	1003:1027	effective DspB substrates	1003:1027	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	5	5	theme	DspB	1013:1016	arg1	analogs					985:991	These analogs	979:991	These analogs	979:991	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	4	6	theme	defined	949:955	arg1	patterns					969:976	defined acetylation patterns	949:976	defined acetylation patterns	949:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	2	7	from	assembly	382:389	arg1	development					424:434	biofilm development	416:434	biofilm development	416:434	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	4	8	from	role	750:753	arg1	recognition					798:808	substrate recognition	788:808	substrate recognition by DspB	788:816	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	1	9	theme	Gram-positive	289:301	arg1	pathogens					327:335	both Gram-positive and Gram-negative human pathogens	284:335	both Gram-positive and Gram-negative human pathogens	284:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	6	10	theme	disaccharide	1484:1495	arg1	product					1497:1503	a nonreducing disaccharide product	1470:1503	a nonreducing disaccharide product	1470:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	5	11	theme	specific	1078:1085	arg1	pattern					1114:1120	the specific substrate de-N-acetylation pattern	1074:1120	the specific substrate de-N-acetylation pattern	1074:1120	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	6	12	theme	nonreducing	1392:1402	arg1	GlcN					1404:1407	a nonreducing GlcN	1390:1407	a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product	1390:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	6	13	theme	exoglycosidic	1263:1275	arg1	activity					1286:1293	a primarily exoglycosidic cleavage activity	1251:1293	a primarily exoglycosidic cleavage activity	1251:1293	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	5	14	theme	hydrolysis	1046:1055	arg1	rate					1038:1041	the rate	1034:1041	the rate of hydrolysis	1034:1055	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	5	14	theme	hydrolysis	1046:1055	arg1	dependent					1061:1069	dependent	1061:1069	dependent	1061:1069	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	6	15	theme	nonreducing	1472:1482	arg1	product					1497:1503	a nonreducing disaccharide product	1470:1503	a nonreducing disaccharide product	1470:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	3	16	theme	dPNAG	616:620	arg1	degradation					601:611	The enzymatic degradation	587:611	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	587:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	7	17	theme	glycosidase	1599:1609	arg1	specificity					1569:1579	the substrate specificity	1555:1579	the substrate specificity of dPNAG specific glycosidase	1555:1609	These observations provide critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts.					
31430121	6	18	theme	assisted	1317:1324	arg1	mechanism					1335:1343	a substrate assisted cleavage mechanism	1305:1343	a substrate assisted cleavage mechanism	1305:1343	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	2	19	from	function	395:402	arg1	development					424:434	biofilm development	416:434	biofilm development	416:434	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	3	20	theme	enzymatic	591:599	arg1	degradation					601:611	The enzymatic degradation	587:611	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	587:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	1	21	theme	Gram-negative	307:319	arg1	pathogens					327:335	both Gram-positive and Gram-negative human pathogens	284:335	both Gram-positive and Gram-negative human pathogens	284:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	5	22	theme	cleavage	1175:1182	arg1	cleavage					1175:1182	cleavage	1175:1182	cleavage	1175:1182	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	5	22	theme	cleavage	1175:1182	arg1	site					1167:1170	the site	1163:1170	the site of cleavage	1163:1182	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	4	23	theme	trisaccharide	922:934	arg1	analogs					936:942	dPNAG trisaccharide analogs	916:942	dPNAG trisaccharide analogs with defined acetylation patterns	916:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	4	24	theme	preactivation-based	843:861	arg1	approach					885:892	an efficient preactivation-based one-pot glycosylation approach	830:892	an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns	830:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	2	25	theme	different	452:460	arg1	patterns					462:469	different patterns	452:469	different patterns of deacetylation	452:486	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	2	26	theme	Dispersin	567:575	arg1	B					577:577	Dispersin B	567:577	Dispersin B (DspB)	567:584	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	2	26	theme	Dispersin	567:575	arg1	DspB					580:583	DspB	580:583	DspB	580:583	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	7	27	theme	dPNAG	1584:1588	arg1	glycosidase					1599:1609	dPNAG specific glycosidase	1584:1609	dPNAG specific glycosidase	1584:1609	These observations provide critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts.					
31430121	4	28	theme	dPNAG	916:920	arg1	analogs					936:942	dPNAG trisaccharide analogs	916:942	dPNAG trisaccharide analogs with defined acetylation patterns	916:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	2	29	theme	proper	375:380	arg1	assembly					382:389	proper assembly	375:389	proper assembly	375:389	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	6	30	dep	cleaved	1419:1425	arg1	leading					1432:1438	leading	1432:1438	leading to the exclusive formation of a nonreducing disaccharide product	1432:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	6	31	theme	product	1497:1503	arg1	formation					1457:1465	the exclusive formation	1443:1465	the exclusive formation of a nonreducing disaccharide product	1443:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	5	32	theme	substrate	1087:1095	arg1	pattern					1114:1120	the specific substrate de-N-acetylation pattern	1074:1120	the specific substrate de-N-acetylation pattern	1074:1120	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	2	33	dep	assembly	382:389	arg1	the					371:373	the	371:373	the	371:373	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	1	34	theme	key	206:208	arg1	Exopolysaccharides					99:116	Exopolysaccharides	99:116	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	99:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	34	theme	key	206:208	arg1	components					221:230	key structural components	206:230	key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens	206:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	0	35	theme	Differential	0:11	arg1	Recognition					13:23	Differential Recognition	0:23	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase	0:96	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	1	36	theme	human	321:325	arg1	pathogens					327:335	both Gram-positive and Gram-negative human pathogens	284:335	both Gram-positive and Gram-negative human pathogens	284:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	4	37	theme	substrate	788:796	arg1	recognition					798:808	substrate recognition	788:808	substrate recognition by DspB	788:816	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	6	38	theme	exclusive	1447:1455	arg1	formation					1457:1465	the exclusive formation	1443:1465	the exclusive formation of a nonreducing disaccharide product	1443:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	1	39	theme	structural	210:219	arg1	Exopolysaccharides					99:116	Exopolysaccharides	99:116	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	99:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	39	theme	structural	210:219	arg1	components					221:230	key structural components	206:230	key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens	206:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	0	40	theme	Deacetylated	28:39	arg1	Oligosaccharides					46:61	Deacetylated PNAG Oligosaccharides	28:61	Deacetylated PNAG Oligosaccharides	28:61	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	1	41	theme	pathogens	327:335	arg1	Exopolysaccharides					99:116	Exopolysaccharides	99:116	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	99:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	41	theme	pathogens	327:335	arg1	components					221:230	key structural components	206:230	key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens	206:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	4	42	theme	substrate	758:766	arg1	de-N-acetylation					768:783	substrate de-N-acetylation	758:783	substrate de-N-acetylation	758:783	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	6	43	theme	cleavage	1277:1284	arg1	activity					1286:1293	a primarily exoglycosidic cleavage activity	1251:1293	a primarily exoglycosidic cleavage activity	1251:1293	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	7	44	theme	critical	1533:1540	arg1	insight					1542:1548	critical insight	1533:1548	critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts	1533:1658	These observations provide critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts.					
31430121	6	45	theme	substrate	1307:1315	arg1	mechanism					1335:1343	a substrate assisted cleavage mechanism	1305:1343	a substrate assisted cleavage mechanism	1305:1343	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	2	46	theme	deacetylation	474:486	arg1	patterns					462:469	different patterns	452:469	different patterns of deacetylation	452:486	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	0	47	theme	Oligosaccharides	46:61	arg1	Recognition					13:23	Differential Recognition	0:23	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase	0:96	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	4	48	theme	de-N-acetylation	768:783	arg1	role					750:753	the role	746:753	the role of substrate de-N-acetylation on substrate recognition by DspB	746:816	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	3	49	theme	biofilm	719:725	arg1	dispersal					727:735	biofilm dispersal	719:735	biofilm dispersal	719:735	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	3	50	theme	actinomycetemcomitans	645:665	arg1	DspB					698:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	6	51	contain	containing	1379:1388	arg1	substrates					1368:1377	substrates	1368:1377	substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product	1368:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	6	51	contain	containing	1379:1388	arg2	GlcN					1404:1407	a nonreducing GlcN	1390:1407	a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product	1390:1503	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	0	52	theme	PNAG	41:44	arg1	Oligosaccharides					46:61	Deacetylated PNAG Oligosaccharides	28:61	Deacetylated PNAG Oligosaccharides	28:61	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	3	53	theme	native	667:672	arg1	DspB					698:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	1	54	theme	biofilm	239:245	arg1	substance					271:279	the biofilm extracellular polymeric substance	235:279	the biofilm extracellular polymeric substance	235:279	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	4	55	theme	analogs	936:942	arg1	panel					907:911	a panel	905:911	a panel of dPNAG trisaccharide analogs with defined acetylation patterns	905:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	1	56	theme	de-N-acetylated	142:156	arg1	-N-acetyl-glucosamine					172:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine	132:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	132:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	56	theme	de-N-acetylated	142:156	arg1	dPNAG					195:199	dPNAG	195:199	dPNAG	195:199	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	3	57	theme	Aggregatibacter	629:643	arg1	DspB					698:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	1	58	theme	poly-β-d-	158:166	arg1	-N-acetyl-glucosamine					172:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine	132:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	132:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	58	theme	poly-β-d-	158:166	arg1	dPNAG					195:199	dPNAG	195:199	dPNAG	195:199	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	5	59	theme	de-N-acetylation	1097:1112	arg1	pattern					1114:1120	the specific substrate de-N-acetylation pattern	1074:1120	the specific substrate de-N-acetylation pattern	1074:1120	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	7	60	theme	substrate	1559:1567	arg1	specificity					1569:1579	the substrate specificity	1555:1579	the substrate specificity of dPNAG specific glycosidase	1555:1609	These observations provide critical insight into the substrate specificity of dPNAG specific glycosidase that can help guide their design as biocatalysts.					
31430121	6	61	theme	cleavage	1326:1333	arg1	mechanism					1335:1343	a substrate assisted cleavage mechanism	1305:1343	a substrate assisted cleavage mechanism	1305:1343	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	4	62	theme	glycosylation	871:883	arg1	approach					885:892	an efficient preactivation-based one-pot glycosylation approach	830:892	an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns	830:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	1	63	theme	extracellular	247:259	arg1	substance					271:279	the biofilm extracellular polymeric substance	235:279	the biofilm extracellular polymeric substance	235:279	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	4	64	theme	efficient	833:841	arg1	approach					885:892	an efficient preactivation-based one-pot glycosylation approach	830:892	an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns	830:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	5	65	theme	effective	1003:1011	arg1	substrates					1018:1027	effective DspB substrates	1003:1027	effective DspB substrates	1003:1027	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	5	65	theme	effective	1003:1011	arg1	analogs					985:991	These analogs	979:991	These analogs	979:991	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	4	66	theme	one-pot	863:869	arg1	approach					885:892	an efficient preactivation-based one-pot glycosylation approach	830:892	an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns	830:976	To test the role of substrate de-N-acetylation on substrate recognition by DspB, we applied an efficient preactivation-based one-pot glycosylation approach to prepare a panel of dPNAG trisaccharide analogs with defined acetylation patterns.					
31430121	1	67	theme	1→6	168:170	arg1	-N-acetyl-glucosamine					172:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine	132:192	partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	132:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	67	theme	1→6	168:170	arg1	dPNAG					195:199	dPNAG	195:199	dPNAG	195:199	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	6	68	theme	product	1221:1227	arg1	distributions					1229:1241	The product distributions	1217:1241	The product distributions	1217:1241	The product distributions support a primarily exoglycosidic cleavage activity following a substrate assisted cleavage mechanism, with the exception of substrates containing a nonreducing GlcN that were cleaved endo leading to the exclusive formation of a nonreducing disaccharide product.					
31430121	1	69	theme	polymeric	261:269	arg1	substance					271:279	the biofilm extracellular polymeric substance	235:279	the biofilm extracellular polymeric substance	235:279	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	0	70	theme	Degrading	76:84	arg1	Glycosidase					86:96	a Biofilm Degrading Glycosidase	66:96	a Biofilm Degrading Glycosidase	66:96	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	5	71	theme	located	1147:1153	arg1	+2					1155:1156	glucosamine (GlcN) located +2	1128:1156	glucosamine (GlcN) located +2 from the site of cleavage	1128:1182	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
31430121	3	72	theme	β-hexosaminidase	674:689	arg1	DspB					698:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	2	73	theme	biofilm	416:422	arg1	development					424:434	biofilm development	416:434	biofilm development	416:434	De-N-acetylation is required for the proper assembly and function of dPNAG in biofilm development suggesting that different patterns of deacetylation may be preferentially recognized by proteins that interact with dPNAG, such as Dispersin B (DspB).					
31430121	1	74	theme	substance	271:279	arg1	Exopolysaccharides					99:116	Exopolysaccharides	99:116	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG)	99:200	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	1	74	theme	substance	271:279	arg1	components					221:230	key structural components	206:230	key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens	206:335	Exopolysaccharides consisting of partially de-N-acetylated poly-β-d-(1→6)-N-acetyl-glucosamine (dPNAG) are key structural components of the biofilm extracellular polymeric substance of both Gram-positive and Gram-negative human pathogens.					
31430121	0	75	theme	Biofilm	68:74	arg1	Glycosidase					86:96	a Biofilm Degrading Glycosidase	66:96	a Biofilm Degrading Glycosidase	66:96	Differential Recognition of Deacetylated PNAG Oligosaccharides by a Biofilm Degrading Glycosidase.					
31430121	3	76	theme	enzyme	691:696	arg1	DspB					698:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB	625:701	The enzymatic degradation of dPNAG by the Aggregatibacter actinomycetemcomitans native β-hexosaminidase enzyme DspB plays a role in biofilm dispersal.					
31430121	5	77	from	site	1167:1170	arg1	+2					1155:1156	glucosamine (GlcN) located +2	1128:1156	glucosamine (GlcN) located +2 from the site of cleavage	1128:1182	These analogs served as effective DspB substrates, and the rate of hydrolysis was dependent on the specific substrate de-N-acetylation pattern, with glucosamine (GlcN) located +2 from the site of cleavage being preferentially hydrolyzed.					
29389054	4	0	theme	various	704:710	arg1	appendages					725:734	various carbohydrate appendages	704:734	various carbohydrate appendages	704:734	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	1	1	theme	wall	141:144	arg1	acids					74:78	Teichoic acids	65:78	Teichoic acids (TAs)	65:84	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	1	theme	wall	141:144	arg1	components					94:103	key components	90:103	key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages	90:250	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	2	theme	carbohydrate	228:239	arg1	appendages					241:250	carbohydrate appendages	228:250	carbohydrate appendages	228:250	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	5	3	theme	diverse	791:797	arg1	TA-microarray					799:811	a structurally diverse TA-microarray	776:811	a structurally diverse TA-microarray	776:811	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	5	4	used	used	761:764	arg2	These					751:755	These	751:755	These	751:755	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	3	5	theme	TA	538:539	arg1	oligomers					541:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	4	6	theme	"	587:587	arg1	system					596:601	a "universal" linker system and fluorous tag purification strategy	575:640	system	596:601	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	3	7	theme	oligomers	541:549	arg1	generation					482:491	the rapid generation	472:491	the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	472:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	5	8	theme	anti-LTA	886:893	arg1	antibodies					916:925	anti-LTA (lipoteichoic acids) antibodies	886:925	anti-LTA (lipoteichoic acids) antibodies	886:925	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	2	9	theme	pure	291:294	arg1	TAs					309:311	pure well-defined TAs	291:311	pure well-defined TAs for biological or immunological evaluation	291:354	Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources.					
29389054	1	10	theme	Teichoic	65:72	arg1	acids					74:78	Teichoic acids	65:78	Teichoic acids (TAs)	65:84	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	10	theme	Teichoic	65:72	arg1	components					94:103	key components	90:103	key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages	90:250	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	10	theme	Teichoic	65:72	arg1	TAs					81:83	TAs	81:83	TAs	81:83	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	11	theme	Gram-positive	112:124	arg1	wall					141:144	the Gram-positive bacterial cell wall	108:144	the Gram-positive bacterial cell wall	108:144	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	4	12	theme	carbohydrate	712:723	arg1	appendages					725:734	various carbohydrate appendages	704:734	various carbohydrate appendages	704:734	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	3	13	dep	glycosylated	509:520	arg1	well-defined					496:507	well-defined	496:507	well-defined	496:507	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	3	13	dep	glycosylated	509:520	arg1	glycerol-based					523:536	glycerol-based	523:536	glycerol-based	523:536	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	5	14	theme	binding	863:869	arg1	preferences					871:881	the binding preferences	859:881	the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level	859:948	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	3	15	theme	synthesis	449:457	arg1	approach					459:466	a streamlined automated solid-phase synthesis approach	413:466	a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	413:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	4	16	theme	system	596:601	arg1	use					568:570	the use	564:570	the use of a "universal" linker system and fluorous tag purification strategy	564:640	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	5	17	theme	lipoteichoic	896:907	arg1	anti-LTA					886:893	anti-LTA	886:893	anti-LTA (lipoteichoic acids) antibodies	886:925	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	5	17	theme	lipoteichoic	896:907	arg1	acids					909:913	lipoteichoic acids	896:913	lipoteichoic acids	896:913	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	4	18	theme	linker	589:594	arg1	system					596:601	a "universal" linker system and fluorous tag purification strategy	575:640	system	596:601	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	4	19	theme	glycerolphosphate	656:672	arg1	pentadecamers					674:686	glycerolphosphate pentadecamers	656:686	glycerolphosphate pentadecamers	656:686	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	3	20	theme	automated	427:435	arg1	approach					459:466	a streamlined automated solid-phase synthesis approach	413:466	a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	413:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	4	21	theme	pentadecamers	674:686	arg1	library					645:651	a library	643:651	a library	643:651	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	3	22	gly	glycosylated	509:520	arg1	oligomers					541:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	2	23	theme	natural	380:386	arg1	sources					388:394	natural sources	380:394	natural sources	380:394	Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources.					
29389054	2	24	theme	well-defined	296:307	arg1	TAs					309:311	pure well-defined TAs	291:311	pure well-defined TAs for biological or immunological evaluation	291:354	Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources.					
29389054	1	25	theme	phosphate	175:183	arg1	units					195:199	alditol phosphate repeating units	167:199	alditol phosphate repeating units	167:199	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	5	26	theme	molecular	934:942	arg1	level					944:948	the molecular level	930:948	the molecular level	930:948	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	4	27	theme	universal	578:586	arg1	system					596:601	a "universal" linker system and fluorous tag purification strategy	575:640	system	596:601	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	1	28	theme	key	90:92	arg1	acids					74:78	Teichoic acids	65:78	Teichoic acids (TAs)	65:84	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	28	theme	key	90:92	arg1	components					94:103	key components	90:103	key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages	90:250	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	1	29	theme	repeating	185:193	arg1	units					195:199	alditol phosphate repeating units	167:199	alditol phosphate repeating units	167:199	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	0	30	theme	Teichoic	40:47	arg1	Fragments					54:62	Teichoic Acid Fragments	40:62	Teichoic Acid Fragments	40:62	Streamlined Synthesis and Evaluation of Teichoic Acid Fragments.					
29389054	3	31	theme	solid-phase	437:447	arg1	approach					459:466	a streamlined automated solid-phase synthesis approach	413:466	a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	413:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	5	32	theme	first	847:851	arg1	time					853:856	the first time	843:856	the first time	843:856	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	3	33	theme	streamlined	415:425	arg1	approach					459:466	a streamlined automated solid-phase synthesis approach	413:466	a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	413:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	0	34	theme	Fragments	54:62	arg1	Evaluation					26:35	Evaluation	26:35	Evaluation	26:35	Streamlined Synthesis and Evaluation of Teichoic Acid Fragments.					
29389054	0	34	theme	Fragments	54:62	arg1	Synthesis					12:20	Synthesis	12:20	Synthesis	12:20	Streamlined Synthesis and Evaluation of Teichoic Acid Fragments.					
29389054	1	35	theme	alditol	167:173	arg1	units					195:199	alditol phosphate repeating units	167:199	alditol phosphate repeating units	167:199	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	3	36	theme	glycosylated	509:520	arg1	oligomers					541:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	well-defined glycosylated, glycerol-based TA oligomers	496:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	0	37	theme	Acid	49:52	arg1	Fragments					54:62	Teichoic Acid Fragments	40:62	Teichoic Acid Fragments	40:62	Streamlined Synthesis and Evaluation of Teichoic Acid Fragments.					
29389054	2	38	theme	immunological	331:343	arg1	evaluation					345:354	biological or immunological evaluation	317:354	biological or immunological evaluation	317:354	Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources.					
29389054	5	39	from	level	944:948	arg1	preferences					871:881	the binding preferences	859:881	the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level	859:948	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	5	40	theme	antibodies	916:925	arg1	preferences					871:881	the binding preferences	859:881	the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level	859:948	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	4	41	theme	tag	616:618	arg1	strategy					633:640	a "universal" linker system and fluorous tag purification strategy	575:640	strategy	633:640	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	5	42	used	used	823:826	arg2	TA-microarray					799:811	a structurally diverse TA-microarray	776:811	a structurally diverse TA-microarray	776:811	These are used to create a structurally diverse TA-microarray, which is used to reveal, for the first time, the binding preferences of anti-LTA (lipoteichoic acids) antibodies at the molecular level.					
29389054	2	43	theme	biological	317:326	arg1	evaluation					345:354	biological or immunological evaluation	317:354	biological or immunological evaluation	317:354	Because of their microhetereogeneity, pure well-defined TAs for biological or immunological evaluation cannot be obtained from natural sources.					
29389054	4	44	theme	fluorous	607:614	arg1	strategy					633:640	a "universal" linker system and fluorous tag purification strategy	575:640	strategy	633:640	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	3	45	theme	rapid	476:480	arg1	generation					482:491	the rapid generation	472:491	the rapid generation of well-defined glycosylated, glycerol-based TA oligomers	472:549	We present here a streamlined automated solid-phase synthesis approach for the rapid generation of well-defined glycosylated, glycerol-based TA oligomers.					
29389054	1	46	theme	bacterial	126:134	arg1	wall					141:144	the Gram-positive bacterial cell wall	108:144	the Gram-positive bacterial cell wall	108:144	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	4	47	theme	strategy	633:640	arg1	use					568:570	the use	564:570	the use of a "universal" linker system and fluorous tag purification strategy	564:640	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
29389054	1	48	theme	cell	136:139	arg1	wall					141:144	the Gram-positive bacterial cell wall	108:144	the Gram-positive bacterial cell wall	108:144	Teichoic acids (TAs) are key components of the Gram-positive bacterial cell wall that are composed of alditol phosphate repeating units, decorated with alanine or carbohydrate appendages.					
29389054	4	49	theme	purification	620:631	arg1	strategy					633:640	a "universal" linker system and fluorous tag purification strategy	575:640	strategy	633:640	Building on the use of a "universal" linker system and fluorous tag purification strategy, a library of glycerolphosphate pentadecamers, decorated with various carbohydrate appendages, is generated.					
31986272	9	0	theme	relative	1721:1728	arg1	quantitation					1730:1741	the relative quantitation	1717:1741	the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach	1717:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	1	1	theme	necessary	258:266	arg1	elucidation					299:309	support structural elucidation	280:309	support structural elucidation	280:309	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	1	theme	necessary	258:266	arg1	quantitation					324:335	relative quantitation	315:335	relative quantitation of glycans released from therapeutics	315:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	1	theme	necessary	258:266	arg1	throughput					268:277	the necessary throughput	254:277	the necessary throughput	254:277	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	7	2	theme	glycan	1491:1496	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	3	theme	same	1452:1455	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	3	theme	same	1452:1455	arg1	amount					1457:1462	the same amount	1448:1462	the same amount of light and heavy labeled glycan pools	1448:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	8	4	from	ratio	1546:1550	arg1	range					1582:1586	the range	1578:1586	the range of 0.97 ≤ x ≤ 1.05	1578:1605	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	7	5	theme	heavy	1477:1481	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	10	6	theme	single	2120:2125	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	10	7	theme	fragment	2149:2156	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	3	8	theme	reagent	737:743	arg1	light					705:709	light	705:709	light	705:709	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	9	9	theme	twoplex	1805:1811	arg1	approach					1813:1820	the twoplex approach	1801:1820	the twoplex approach	1801:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	1	10	theme	glycosylation	160:172	arg1	analysis					174:181	Comparative glycosylation analysis	148:181	Comparative glycosylation analysis of biopharmaceuticals	148:203	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	7	11	theme	light	1467:1471	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	0	12	link	N-linked	41:48	arg1	analysis					57:64	N-linked glycan analysis	41:64	N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid	41:145	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	9	13	theme	quantitation	1730:1741	arg1	data					1743:1746	the relative quantitation data	1717:1746	the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach	1717:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	14	theme	Excellent	1687:1695	arg1	correlation					1697:1707	Excellent correlation	1687:1707	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram	1687:1904	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	6	15	theme	chromatographic	1162:1176	arg1	selectivity					1178:1188	chromatographic selectivity	1162:1188	chromatographic selectivity	1162:1188	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	10	16	theme	oligosaccharides	2207:2222	arg1	interpretation					2185:2198	structural interpretation	2174:2198	structural interpretation of the oligosaccharides	2174:2222	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	3	17	theme	labelling	651:659	arg1	efficiency					661:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	2	18	theme	acquisition	623:633	arg1	mode					635:638	data dependent acquisition mode	608:638	data dependent acquisition mode	608:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	5	19	theme	glycan	947:952	arg1	profiles					954:961	glycan profiles	947:961	glycan profiles of two human IgG lots	947:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	2	20	theme	data	608:611	arg1	mode					635:638	data dependent acquisition mode	608:638	data dependent acquisition mode	608:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	0	21	theme	acid	142:145	arg1	isotopologues					102:114	light (12C6) and heavy (13C6) isotopologues	72:114	isotopologues	102:114	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	0	21	theme	acid	142:145	arg1	12C6					79:82	light (12C6) and heavy (13C6) isotopologues	72:114	12C6	79:82	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	3	22	theme	%	676:676	arg1	efficiency					661:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	9	23	theme	N-glycans	1751:1759	arg1	data					1743:1746	the relative quantitation data	1717:1746	the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach	1717:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	23	theme	N-glycans	1751:1759	arg1	ratios					1826:1831	ratios	1826:1831	ratios	1826:1831	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	24	theme	potential	1992:2000	arg1	applicability					2002:2014	its potential applicability	1988:2014	its potential applicability in the biopharmaceutical industry	1988:2048	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	0	25	theme	analysis	57:64	arg1	twoplexing					27:36	twoplexing	27:36	twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid	27:145	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	5	26	theme	Peak	920:923	arg1	distributions					930:942	Peak area distributions	920:942	Peak area distributions of glycan profiles of two human IgG lots	920:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	8	27	theme	MS	1505:1506	arg1	analyses					1508:1515	MS analyses	1505:1515	MS analyses of each glycan	1505:1530	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	0	28	theme	light	72:76	arg1	12C6					79:82	light (12C6) and heavy (13C6) isotopologues	72:114	12C6	79:82	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	9	29	theme	IgG	1776:1778	arg1	pools					1789:1793	two human IgG N-glycan pools	1766:1793	two human IgG N-glycan pools using the twoplex approach	1766:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	30	theme	biopharmaceutical	2023:2039	arg1	industry					2041:2048	the biopharmaceutical industry	2019:2048	the biopharmaceutical industry	2019:2048	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	10	31	theme	informative	2058:2068	arg1	spectra					2078:2084	Highly informative HCD-MS2 spectra	2051:2084	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions	2051:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	7	32	theme	relative	1234:1241	arg1	quantitation					1243:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	33	theme	amount	1457:1462	arg1	clean-up					1436:1443	clean-up	1436:1443	clean-up	1436:1443	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	33	theme	amount	1457:1462	arg1	mixing					1425:1430	mixing	1425:1430	mixing	1425:1430	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	2	34	theme	wet	551:553	arg1	conditions					564:573	wet labeling conditions	551:573	wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode	551:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	8	35	theme	glycan	1525:1530	arg1	analyses					1508:1515	MS analyses	1505:1515	MS analyses of each glycan	1505:1530	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	2	36	theme	applicability	423:435	arg1	assessment					437:446	applicability assessment	423:446	applicability assessment	423:446	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	7	37	theme	MS1	1224:1226	arg1	quantitation					1243:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	6	38	theme	peak	1097:1100	arg1	distribution					1107:1118	peak area distribution	1097:1118	peak area distribution across the three dyes	1097:1140	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	1	39	theme	support	280:286	arg1	elucidation					299:309	support structural elucidation	280:309	support structural elucidation	280:309	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	39	theme	support	280:286	arg1	throughput					268:277	the necessary throughput	254:277	the necessary throughput	254:277	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	5	40	theme	human	970:974	arg1	lots					980:983	two human IgG lots	966:983	two human IgG lots	966:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	4	41	theme	IgG	780:782	arg1	lots					784:787	two human IgG lots	770:787	two human IgG lots labeled by light and heavy isotopolouges	770:828	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	2	42	theme	twoplex	453:459	arg1	approach					461:468	a twoplex approach	451:468	a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode	451:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	9	43	from	pools	1789:1793	arg1	data					1743:1746	the relative quantitation data	1717:1746	the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach	1717:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	43	from	pools	1789:1793	arg1	ratios					1826:1831	ratios	1826:1831	ratios	1826:1831	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	5	44	theme	lots	980:983	arg1	profiles					954:961	glycan profiles	947:961	glycan profiles of two human IgG lots	947:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	2	45	theme	3-aminobenzenesulfonic	509:530	arg1	3-ASA					538:542	3-ASA	538:542	3-ASA	538:542	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	2	45	theme	3-aminobenzenesulfonic	509:530	arg1	acid					532:535	3-aminobenzenesulfonic acid	509:535	3-aminobenzenesulfonic acid (3-ASA)	509:543	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	8	46	theme	light	1555:1559	arg1	ratio					1546:1550	a ratio	1544:1550	a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05	1544:1605	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	1	47	theme	relative	315:322	arg1	quantitation					324:335	relative quantitation	315:335	relative quantitation of glycans released from therapeutics	315:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	47	theme	relative	315:322	arg1	throughput					268:277	the necessary throughput	254:277	the necessary throughput	254:277	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	7	48	theme	IgG	1326:1328	arg1	lot					1313:1315	the same lot	1304:1315	the same lot of human IgG	1304:1328	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	48	theme	IgG	1326:1328	arg1	IgG					1326:1328	human IgG	1320:1328	human IgG	1320:1328	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	9	49	theme	peak	1838:1841	arg1	distribution					1848:1859	peak area distribution	1838:1859	peak area distribution calculated from the fluorescent chromatogram	1838:1904	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	8	50	theme	heavy	1565:1569	arg1	XIC					1571:1573	heavy XIC	1565:1573	heavy XIC	1565:1573	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	0	51	theme	N-linked	41:48	arg1	analysis					57:64	N-linked glycan analysis	41:64	N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid	41:145	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	10	52	theme	Y-	2106:2107	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	2	53	dep	development	407:417	arg1	the					403:405	the	403:405	the	403:405	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	7	54	theme	same	1308:1311	arg1	lot					1313:1315	the same lot	1304:1315	the same lot of human IgG	1304:1328	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	54	theme	same	1308:1311	arg1	IgG					1326:1328	human IgG	1320:1328	human IgG	1320:1328	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	55	theme	pools	1498:1502	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	7	55	theme	pools	1498:1502	arg1	amount					1457:1462	the same amount	1448:1462	the same amount of light and heavy labeled glycan pools	1448:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	9	56	theme	fluorescent	1881:1891	arg1	chromatogram					1893:1904	the fluorescent chromatogram	1877:1904	the fluorescent chromatogram	1877:1904	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	8	57	theme	0.97 ≤ x ≤ 1.05	1591:1605	arg1	range					1582:1586	the range	1578:1586	the range of 0.97 ≤ x ≤ 1.05	1578:1605	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	1	58	gly	glycosylation	160:172	arg1	biopharmaceuticals					186:203	biopharmaceuticals	186:203	biopharmaceuticals	186:203	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	2	59	theme	current	380:386	arg1	study					388:392	The current study	376:392	The current study	376:392	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	7	60	theme	labeled	1483:1489	arg1	pools					1498:1502	light and heavy labeled glycan pools	1467:1502	light and heavy labeled glycan pools	1467:1502	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	10	61	theme	Z-type	2113:2118	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	6	62	from	results	1086:1092	arg1	distribution					1107:1118	peak area distribution	1097:1118	peak area distribution across the three dyes	1097:1140	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	8	63	theme	glycans	1678:1684	arg1	quantitation					1662:1673	the relative quantitation	1649:1673	the relative quantitation of glycans	1649:1684	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	3	64	dep	light	705:709	arg1	the					701:703	the	701:703	the	701:703	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	3	64	dep	light	705:709	arg1	variants					721:728	variants	721:728	variants	721:728	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	6	65	theme	different	1208:1216	arg1	tags					1218:1221	the different tags	1204:1221	the different tags	1204:1221	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	10	66	theme	double	2131:2136	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	1	67	theme	Comparative	148:158	arg1	analysis					174:181	Comparative glycosylation analysis	148:181	Comparative glycosylation analysis of biopharmaceuticals	148:203	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	9	68	theme	area	1843:1846	arg1	distribution					1848:1859	peak area distribution	1838:1859	peak area distribution calculated from the fluorescent chromatogram	1838:1904	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	4	69	theme	labeling	863:870	arg1	bias					872:875	no labeling bias	860:875	no labeling bias introduced by either of the isotopologues	860:917	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	10	70	theme	glycosidic	2138:2147	arg1	ions					2158:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Y- and Z-type single and double glycosidic fragment ions	2106:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	9	71	theme	human	1770:1774	arg1	pools					1789:1793	two human IgG N-glycan pools	1766:1793	two human IgG N-glycan pools using the twoplex approach	1766:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	0	72	theme	13C6	96:99	arg1	isotopologues					102:114	light (12C6) and heavy (13C6) isotopologues	72:114	isotopologues	102:114	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	1	73	theme	biopharmaceuticals	186:203	arg1	analysis					174:181	Comparative glycosylation analysis	148:181	Comparative glycosylation analysis of biopharmaceuticals	148:203	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	2	74	theme	UHPLC-MS	587:594	arg1	analysis					596:603	UHPLC-MS analysis	587:603	UHPLC-MS analysis	587:603	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	10	75	theme	structural	2174:2183	arg1	interpretation					2185:2198	structural interpretation	2174:2198	structural interpretation of the oligosaccharides	2174:2222	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	3	76	theme	Excellent	641:649	arg1	efficiency					661:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency	641:670	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	9	77	from	reliability	1958:1968	arg1	industry					2041:2048	the biopharmaceutical industry	2019:2048	the biopharmaceutical industry	2019:2048	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	0	78	theme	3-aminobenzenesulfonic	119:140	arg1	acid					142:145	3-aminobenzenesulfonic acid	119:145	3-aminobenzenesulfonic acid	119:145	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	2	79	theme	dependent	613:621	arg1	mode					635:638	data dependent acquisition mode	608:638	data dependent acquisition mode	608:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	9	80	theme	method	1977:1982	arg1	applicability					2002:2014	its potential applicability	1988:2014	its potential applicability in the biopharmaceutical industry	1988:2048	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	80	theme	method	1977:1982	arg1	reliability					1958:1968	the reliability	1954:1968	the reliability of the method	1954:1982	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	4	81	theme	heavy	810:814	arg1	isotopolouges					816:828	heavy isotopolouges	810:828	heavy isotopolouges	810:828	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	3	82	theme	>90	673:675	arg1	%					676:676	%	676:676	%	676:676	Excellent labelling efficiency, >90%, was achieved for both the light and heavy variants of the reagent.					
31986272	5	83	theme	profiles	954:961	arg1	distributions					930:942	Peak area distributions	920:942	Peak area distributions of glycan profiles of two human IgG lots	920:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	9	84	from	distribution	1848:1859	arg1	data					1743:1746	the relative quantitation data	1717:1746	the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach	1717:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	9	84	from	distribution	1848:1859	arg1	ratios					1826:1831	ratios	1826:1831	ratios	1826:1831	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	7	85	theme	heavy	1381:1385	arg1	isotopologues					1387:1399	light and heavy isotopologues	1371:1399	light and heavy isotopologues	1371:1399	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	4	86	theme	Glycan	746:751	arg1	distributions					753:765	Glycan distributions	746:765	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges	746:828	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	7	87	theme	light	1371:1375	arg1	isotopologues					1387:1399	light and heavy isotopologues	1371:1399	light and heavy isotopologues	1371:1399	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	9	88	theme	N-glycan	1780:1787	arg1	pools					1789:1793	two human IgG N-glycan pools	1766:1793	two human IgG N-glycan pools using the twoplex approach	1766:1820	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	5	89	theme	area	925:928	arg1	distributions					930:942	Peak area distributions	920:942	Peak area distributions of glycan profiles of two human IgG lots	920:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	2	90	theme	acid	532:535	arg1	isotopolouges					492:504	light and heavy isotopolouges	476:504	light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA)	476:543	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	10	91	theme	HCD-MS2	2070:2076	arg1	spectra					2078:2084	Highly informative HCD-MS2 spectra	2051:2084	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions	2051:2161	Highly informative HCD-MS2 spectra dominated mostly by Y- and Z-type single and double glycosidic fragment ions facilitate structural interpretation of the oligosaccharides.					
31986272	7	92	theme	based	1228:1232	arg1	quantitation					1243:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation	1224:1254	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	2	93	theme	labeling	555:562	arg1	conditions					564:573	wet labeling conditions	551:573	wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode	551:638	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	4	94	theme	lots	784:787	arg1	distributions					753:765	Glycan distributions	746:765	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges	746:828	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	0	95	theme	heavy	89:93	arg1	isotopologues					102:114	light (12C6) and heavy (13C6) isotopologues	72:114	isotopologues	102:114	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	6	96	theme	area	1102:1105	arg1	distribution					1107:1118	peak area distribution	1097:1118	peak area distribution across the three dyes	1097:1140	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	0	97	theme	streamlined	2:12	arg1	workflow					14:21	A streamlined workflow	0:21	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.	0:146	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	9	98	dep	observed	1910:1917	arg1	r = 0.986					1920:1928	r = 0.986	1920:1928	r = 0.986	1920:1928	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	7	99	theme	glycan	1336:1341	arg1	pools					1343:1347	glycan pools	1336:1347	glycan pools obtained labeled with light and heavy isotopologues separately	1336:1410	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	2	100	theme	light	476:480	arg1	isotopolouges					492:504	light and heavy isotopolouges	476:504	light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA)	476:543	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	1	101	theme	structural	288:297	arg1	elucidation					299:309	support structural elucidation	280:309	support structural elucidation	280:309	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	101	theme	structural	288:297	arg1	throughput					268:277	the necessary throughput	254:277	the necessary throughput	254:277	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	5	102	theme	IgG	976:978	arg1	lots					980:983	two human IgG lots	966:983	two human IgG lots	966:983	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	6	103	theme	identical	1076:1084	arg1	results					1086:1092	identical results	1076:1092	identical results in peak area distribution across the three dyes	1076:1140	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	2	104	theme	approach	461:468	arg1	development					407:417	development	407:417	development	407:417	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	2	104	theme	approach	461:468	arg1	assessment					437:446	applicability assessment	423:446	applicability assessment	423:446	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	4	105	theme	human	774:778	arg1	lots					784:787	two human IgG lots	770:787	two human IgG lots labeled by light and heavy isotopolouges	770:828	Glycan distributions of two human IgG lots labeled by light and heavy isotopolouges were identical, demonstrating no labeling bias introduced by either of the isotopologues.					
31986272	5	106	theme	RapiFluor-MS	1030:1041	arg1	protocols					1043:1051	RapiFluor-MS protocols	1030:1051	RapiFluor-MS protocols	1030:1051	Peak area distributions of glycan profiles of two human IgG lots were compared to 2-aminobenzamide (2-AB) and RapiFluor-MS protocols.					
31986272	7	107	theme	human	1320:1324	arg1	IgG					1326:1328	human IgG	1320:1328	human IgG	1320:1328	MS1 based relative quantitation was further validated by releasing glycans from the same lot of human IgG, with glycan pools obtained labeled with light and heavy isotopologues separately, followed by mixing and clean-up of the same amount of light and heavy labeled glycan pools.					
31986272	9	108	from	applicability	2002:2014	arg1	industry					2041:2048	the biopharmaceutical industry	2019:2048	the biopharmaceutical industry	2019:2048	Excellent correlation between the relative quantitation data of N-glycans from two human IgG N-glycan pools using the twoplex approach and ratios from peak area distribution calculated from the fluorescent chromatogram was observed (r = 0.986), further corroborating the reliability of the method and its potential applicability in the biopharmaceutical industry.					
31986272	8	109	theme	XIC	1571:1573	arg1	ratio					1546:1550	a ratio	1544:1550	a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05	1544:1605	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	0	110	theme	glycan	50:55	arg1	analysis					57:64	N-linked glycan analysis	41:64	N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid	41:145	A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.					
31986272	2	111	theme	heavy	486:490	arg1	isotopolouges					492:504	light and heavy isotopolouges	476:504	light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA)	476:543	The current study presents the development and applicability assessment of a twoplex approach using light and heavy isotopolouges of 3-aminobenzenesulfonic acid (3-ASA) under wet labeling conditions followed by UHPLC-MS analysis in data dependent acquisition mode.					
31986272	6	112	from	differences	1147:1157	arg1	selectivity					1178:1188	chromatographic selectivity	1162:1188	chromatographic selectivity	1162:1188	The comparison led to identical results in peak area distribution across the three dyes, but differences in chromatographic selectivity attributed to the different tags.					
31986272	1	113	attach	released	348:355	arg2	glycans					340:346	glycans	340:346	glycans released from therapeutics	340:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	113	attach	released	348:355	arg1	therapeutics					362:373	therapeutics	362:373	therapeutics	362:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	114	theme	glycans	340:346	arg1	elucidation					299:309	support structural elucidation	280:309	support structural elucidation	280:309	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	114	theme	glycans	340:346	arg1	quantitation					324:335	relative quantitation	315:335	relative quantitation of glycans released from therapeutics	315:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	1	114	theme	glycans	340:346	arg1	throughput					268:277	the necessary throughput	254:277	the necessary throughput	254:277	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31986272	8	115	theme	relative	1653:1660	arg1	quantitation					1662:1673	the relative quantitation	1649:1673	the relative quantitation of glycans	1649:1684	MS analyses of each glycan resulted in a ratio of light and heavy XIC in the range of 0.97 ≤ x ≤ 1.05, demonstrating the method is amenable for the relative quantitation of glycans.					
31986272	1	116	theme	methods	233:239	arg1	development					218:228	the development	214:228	the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics	214:373	Comparative glycosylation analysis of biopharmaceuticals requires the development of methods that deliver the necessary throughput, support structural elucidation and relative quantitation of glycans released from therapeutics.					
31412214	3	0	theme	secretion	495:503	arg1	loss					460:463	a loss	458:463	a loss of glucose-stimulated insulin secretion (GSIS)	458:510	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	4	1	attach	present	639:645	arg2	CD59					631:634	non-GPI-anchored CD59	614:634	non-GPI-anchored CD59	614:634	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	4	1	attach	present	639:645	arg1	cytoplasm					654:662	the cytoplasm	650:662	the cytoplasm	650:662	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	9	2	from	exocytosis	1772:1781	arg1	β-cells					1797:1803	pancreatic β-cells	1786:1803	pancreatic β-cells	1786:1803	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	7	3	theme	mutant	1440:1445	arg1	reticulum					1214:1222	the endoplasmic reticulum	1198:1222	the endoplasmic reticulum to the cytosol	1198:1237	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	3	theme	mutant	1440:1445	arg1	process					1242:1248	a process	1240:1248	a process mediated by recognition of trimmed N-linked oligosaccharides	1240:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	3	theme	mutant	1440:1445	arg1	failure					1401:1407	the failure	1397:1407	the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS	1198:1486	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	4	4	theme	non-GPI-anchored	614:629	arg1	CD59					631:634	non-GPI-anchored CD59	614:634	non-GPI-anchored CD59	614:634	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	9	5	with	interaction	1808:1818	arg1	proteins					1831:1838	SNARE proteins	1825:1838	SNARE proteins	1825:1838	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	5	6	theme	non-GPI-anchored	842:857	arg1	CD59					859:862	cytosolic non-GPI-anchored CD59	832:862	cytosolic non-GPI-anchored CD59	832:862	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	7	7	theme	glycosylation	1421:1433	arg1	mutant					1440:1445	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	1	8	theme	cell	193:196	arg1	inhibitor					206:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor	147:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC)	147:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	8	theme	cell	193:196	arg1	CD59					139:142	CD59	139:142	CD59	139:142	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	0	9	theme	SNARE	123:127	arg1	proteins					129:136	SNARE proteins	123:136	SNARE proteins	123:136	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	3	10	theme	intracellular	532:544	arg1	pool					546:549	an intracellular pool	529:549	an intracellular pool of CD59	529:557	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	5	11	from	involvement	817:827	arg1	GSIS					867:870	GSIS	867:870	GSIS	867:870	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	5	12	theme	glycan	909:914	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	7	13	theme	CD59	1381:1384	arg1	glycosylation					1337:1349	the partial glycosylation	1325:1349	the partial glycosylation of non-GPI-anchored cytosolic CD59	1325:1384	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	4	14	theme	exocytotic	682:691	arg1	protein					729:735	exocytotic protein vesicle-associated membrane protein 2	682:737	exocytotic protein vesicle-associated membrane protein 2	682:737	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	5	15	theme	A	922:922	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	7	16	theme	non-GPI-anchored	1354:1369	arg1	CD59					1381:1384	non-GPI-anchored cytosolic CD59	1354:1384	non-GPI-anchored cytosolic CD59	1354:1384	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	0	17	theme	insulin	62:68	arg1	exocytosis					70:79	insulin exocytosis	62:79	insulin exocytosis in pancreatic β-cells	62:101	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	4	18	from	GSIS	763:766	arg1	cells					771:775	cells	771:775	cells lacking endogenous CD59 expression	771:810	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	9	19	theme	non-GPI-anchored	1712:1727	arg1	isoform					1739:1745	A cryptic non-GPI-anchored cytosolic isoform	1702:1745	A cryptic non-GPI-anchored cytosolic isoform of CD59	1702:1753	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	8	20	theme	undescribed	1529:1539	arg1	existence					1541:1549	the previously undescribed existence	1514:1549	the previously undescribed existence	1514:1549	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	7	21	gly	glycosylation	1337:1349	arg1	CD59					1381:1384	non-GPI-anchored cytosolic CD59	1354:1384	non-GPI-anchored cytosolic CD59	1354:1384	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	6	22	theme	CD59	1091:1094	arg1	requirements					1075:1086	different structural requirements	1054:1086	different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS	1054:1139	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	9	23	theme	CD59	1750:1753	arg1	isoform					1739:1745	A cryptic non-GPI-anchored cytosolic isoform	1702:1745	A cryptic non-GPI-anchored cytosolic isoform of CD59	1702:1753	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	4	24	theme	vesicle-associated	701:718	arg1	protein					729:735	exocytotic protein vesicle-associated membrane protein 2	682:737	exocytotic protein vesicle-associated membrane protein 2	682:737	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	3	25	theme	enzymatic	412:420	arg1	removal					422:428	enzymatic removal	412:428	CD59 knockdown but not enzymatic removal of cell surface CD59	389:449	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	5	26	theme	GPI	933:935	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	7	27	theme	partial	1329:1335	arg1	glycosylation					1337:1349	the partial glycosylation	1325:1349	the partial glycosylation of non-GPI-anchored cytosolic CD59	1325:1384	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	9	28	theme	insulin	1764:1770	arg1	exocytosis					1772:1781	insulin exocytosis	1764:1781	insulin exocytosis in pancreatic β-cells	1764:1803	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	8	29	theme	cytosolic	1571:1579	arg1	CD59					1581:1584	non-GPI-anchored cytosolic CD59	1554:1584	non-GPI-anchored cytosolic CD59	1554:1584	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	0	30	theme	non-GPI-anchored	10:25	arg1	isoform					37:43	A cryptic non-GPI-anchored cytosolic isoform	0:43	A cryptic non-GPI-anchored cytosolic isoform of CD59	0:51	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	6	31	theme	different	1054:1062	arg1	requirements					1075:1086	different structural requirements	1054:1086	different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS	1054:1139	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	5	32	from	dependent	991:999	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	4	33	theme	endogenous	785:794	arg1	expression					801:810	endogenous CD59 expression	785:810	endogenous CD59 expression	785:810	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	3	34	theme	surface	438:444	arg1	CD59					446:449	cell surface CD59	433:449	cell surface CD59	433:449	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	0	35	theme	CD59	48:51	arg1	isoform					37:43	A cryptic non-GPI-anchored cytosolic isoform	0:43	A cryptic non-GPI-anchored cytosolic isoform of CD59	0:51	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	7	36	theme	N-linked	1285:1292	arg1	oligosaccharides					1294:1309	trimmed N-linked oligosaccharides	1277:1309	trimmed N-linked oligosaccharides	1277:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	2	37	theme	pancreatic	319:328	arg1	islets					330:335	pancreatic islets	319:335	pancreatic islets	319:335	We showed previously that CD59 is highly expressed in pancreatic islets but is down-regulated in rodent models of diabetes.					
31412214	5	38	from	β-cells	954:960	arg1	dependent					991:999	dependent	991:999	dependent	991:999	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	1	39	theme	complement	223:232	arg1	MAC					259:261	MAC	259:261	MAC	259:261	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	39	theme	complement	223:232	arg1	complex					250:256	complement membrane attack complex	223:256	the complement membrane attack complex (MAC)	219:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	6	40	theme	MAC	1117:1119	arg1	functions					1106:1114	its 2 functions	1100:1114	its 2 functions	1100:1114	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	6	40	theme	MAC	1117:1119	arg1	inhibition					1121:1130	MAC inhibition	1117:1130	MAC inhibition	1117:1130	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	9	41	theme	SNARE	1825:1829	arg1	proteins					1831:1838	SNARE proteins	1825:1838	SNARE proteins	1825:1838	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	2	42	theme	rodent	362:367	arg1	models					369:374	rodent models	362:374	rodent models of diabetes	362:386	We showed previously that CD59 is highly expressed in pancreatic islets but is down-regulated in rodent models of diabetes.					
31412214	1	43	theme	attack	243:248	arg1	MAC					259:261	MAC	259:261	MAC	259:261	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	43	theme	attack	243:248	arg1	complex					250:256	complement membrane attack complex	223:256	the complement membrane attack complex (MAC)	219:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	7	44	gly	glycosylation	1421:1433	arg2	site					1435:1438	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	44	gly	glycosylation	1421:1433	arg2	mutant					1440:1445	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	4	45	theme	current	580:586	arg1	paper					588:592	this current paper	575:592	this current paper	575:592	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	3	46	theme	insulin	487:493	arg1	secretion					495:503	glucose-stimulated insulin secretion	468:503	glucose-stimulated insulin secretion (GSIS)	468:510	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	3	46	theme	insulin	487:493	arg1	GSIS					506:509	GSIS	506:509	GSIS	506:509	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	4	47	from	present	639:645	arg1	cytoplasm					654:662	the cytoplasm	650:662	the cytoplasm	650:662	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	5	48	theme	CD59	859:862	arg1	involvement					817:827	The involvement	813:827	The involvement of cytosolic non-GPI-anchored CD59 in GSIS	813:870	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	5	49	theme	cytosolic	832:840	arg1	CD59					859:862	cytosolic non-GPI-anchored CD59	832:862	cytosolic non-GPI-anchored CD59	832:862	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	0	50	with	interaction	106:116	arg1	proteins					129:136	SNARE proteins	123:136	SNARE proteins	123:136	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	1	51	theme	-anchored	183:191	arg1	inhibitor					206:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor	147:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC)	147:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	51	theme	-anchored	183:191	arg1	CD59					139:142	CD59	139:142	CD59	139:142	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	7	52	theme	site	1435:1438	arg1	mutant					1440:1445	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	1	53	theme	surface	198:204	arg1	inhibitor					206:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor	147:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC)	147:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	53	theme	surface	198:204	arg1	CD59					139:142	CD59	139:142	CD59	139:142	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	7	54	theme	N-linked	1412:1419	arg1	mutant					1440:1445	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	0	55	from	exocytosis	70:79	arg1	β-cells					95:101	pancreatic β-cells	84:101	pancreatic β-cells	84:101	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	3	56	theme	CD59	554:557	arg1	pool					546:549	an intracellular pool	529:549	an intracellular pool of CD59	529:557	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	5	57	theme	phosphatidylinositol	888:907	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	5	58	theme	class	916:920	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	6	59	theme	site-directed	1015:1027	arg1	mutagenesis					1029:1039	site-directed mutagenesis	1015:1039	site-directed mutagenesis	1015:1039	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	7	60	link	N-linked	1285:1292	arg1	oligosaccharides					1294:1309	trimmed N-linked oligosaccharides	1277:1309	trimmed N-linked oligosaccharides	1277:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	61	link	N-linked	1412:1419	arg1	mutant					1440:1445	N-linked glycosylation site mutant CD59	1412:1450	N-linked glycosylation site mutant CD59	1412:1450	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	62	theme	cytosolic	1371:1379	arg1	CD59					1381:1384	non-GPI-anchored cytosolic CD59	1354:1384	non-GPI-anchored cytosolic CD59	1354:1384	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	0	63	theme	pancreatic	84:93	arg1	β-cells					95:101	pancreatic β-cells	84:101	pancreatic β-cells	84:101	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	9	64	theme	cryptic	1704:1710	arg1	isoform					1739:1745	A cryptic non-GPI-anchored cytosolic isoform	1702:1745	A cryptic non-GPI-anchored cytosolic isoform of CD59	1702:1753	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	7	65	theme	endoplasmic	1202:1212	arg1	reticulum					1214:1222	the endoplasmic reticulum	1198:1222	the endoplasmic reticulum to the cytosol	1198:1237	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	7	65	theme	endoplasmic	1202:1212	arg1	process					1242:1248	a process	1240:1248	a process mediated by recognition of trimmed N-linked oligosaccharides	1240:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	8	66	dep	Rosberg	1639:1645	arg1	C					1699:1699	C	1699:1699	E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C	1635:1699	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	9	67	theme	cytosolic	1729:1737	arg1	isoform					1739:1745	A cryptic non-GPI-anchored cytosolic isoform	1702:1745	A cryptic non-GPI-anchored cytosolic isoform of CD59	1702:1753	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	4	68	located	present	639:645	arg1	cytoplasm					654:662	the cytoplasm	650:662	the cytoplasm	650:662	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	4	68	located	present	639:645	arg2	CD59					631:634	non-GPI-anchored CD59	614:634	non-GPI-anchored CD59	614:634	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	8	69	theme	non-GPI-anchored	1554:1569	arg1	CD59					1581:1584	non-GPI-anchored cytosolic CD59	1554:1584	non-GPI-anchored cytosolic CD59	1554:1584	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	2	70	theme	diabetes	379:386	arg1	models					369:374	rodent models	362:374	rodent models of diabetes	362:386	We showed previously that CD59 is highly expressed in pancreatic islets but is down-regulated in rodent models of diabetes.					
31412214	0	71	theme	cryptic	2:8	arg1	isoform					37:43	A cryptic non-GPI-anchored cytosolic isoform	0:43	A cryptic non-GPI-anchored cytosolic isoform of CD59	0:51	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	5	72	theme	knockout	924:931	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	8	73	theme	CD59	1581:1584	arg1	existence					1541:1549	the previously undescribed existence	1514:1549	the previously undescribed existence	1514:1549	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	4	74	theme	protein	693:699	arg1	protein					729:735	exocytotic protein vesicle-associated membrane protein 2	682:737	exocytotic protein vesicle-associated membrane protein 2	682:737	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	0	75	theme	cytosolic	27:35	arg1	isoform					37:43	A cryptic non-GPI-anchored cytosolic isoform	0:43	A cryptic non-GPI-anchored cytosolic isoform of CD59	0:51	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	5	76	theme	anchor-deficient	937:952	arg1	β-cells					954:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells	888:960	The involvement of cytosolic non-GPI-anchored CD59 in GSIS is supported in phosphatidylinositol glycan class A knockout GPI anchor-deficient β-cells, in which GSIS is still CD59 dependent.					
31412214	6	77	theme	structural	1064:1073	arg1	requirements					1075:1086	different structural requirements	1054:1086	different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS	1054:1139	Furthermore, site-directed mutagenesis demonstrated different structural requirements of CD59 for its 2 functions, MAC inhibition and GSIS.					
31412214	4	78	theme	membrane	720:727	arg1	protein					729:735	exocytotic protein vesicle-associated membrane protein 2	682:737	exocytotic protein vesicle-associated membrane protein 2	682:737	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	7	79	theme	oligosaccharides	1294:1309	arg1	recognition					1262:1272	recognition	1262:1272	recognition of trimmed N-linked oligosaccharides	1262:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	9	80	theme	pancreatic	1786:1795	arg1	β-cells					1797:1803	pancreatic β-cells	1786:1803	pancreatic β-cells	1786:1803	A cryptic non-GPI-anchored cytosolic isoform of CD59 controls insulin exocytosis in pancreatic β-cells by interaction with SNARE proteins.					
31412214	3	81	theme	CD59	446:449	arg1	removal					422:428	enzymatic removal	412:428	CD59 knockdown but not enzymatic removal of cell surface CD59	389:449	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	7	82	theme	trimmed	1277:1283	arg1	oligosaccharides					1294:1309	trimmed N-linked oligosaccharides	1277:1309	trimmed N-linked oligosaccharides	1277:1309	Our results suggest that CD59 is retrotranslocated from the endoplasmic reticulum to the cytosol, a process mediated by recognition of trimmed N-linked oligosaccharides, supported by the partial glycosylation of non-GPI-anchored cytosolic CD59 as well as the failure of N-linked glycosylation site mutant CD59 to reach the cytosol or rescue GSIS.					
31412214	8	83	theme	insulin	1609:1615	arg1	secretion.-Golec					1617:1632	insulin secretion.-Golec	1609:1632	insulin secretion.-Golec	1609:1632	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	8	83	theme	insulin	1609:1615	arg1	Rosberg					1639:1645	Rosberg	1639:1645	Rosberg	1639:1645	This study thus proposes the previously undescribed existence of non-GPI-anchored cytosolic CD59, which is required for insulin secretion.-Golec, E., Rosberg, R., Zhang, E., Renström, E., Blom, A. M., King, B. C.					
31412214	4	84	theme	CD59	796:799	arg1	expression					801:810	endogenous CD59 expression	785:810	endogenous CD59 expression	785:810	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	3	85	theme	glucose-stimulated	468:485	arg1	secretion					495:503	glucose-stimulated insulin secretion	468:503	glucose-stimulated insulin secretion (GSIS)	468:510	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	3	85	theme	glucose-stimulated	468:485	arg1	GSIS					506:509	GSIS	506:509	GSIS	506:509	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	1	86	theme	membrane	234:241	arg1	MAC					259:261	MAC	259:261	MAC	259:261	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	86	theme	membrane	234:241	arg1	complex					250:256	complement membrane attack complex	223:256	the complement membrane attack complex (MAC)	219:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	3	87	theme	cell	433:436	arg1	CD59					446:449	cell surface CD59	433:449	cell surface CD59	433:449	CD59 knockdown but not enzymatic removal of cell surface CD59 led to a loss of glucose-stimulated insulin secretion (GSIS), suggesting that an intracellular pool of CD59 is required.					
31412214	4	88	from	cytoplasm	654:662	arg1	present					639:645	present	639:645	present	639:645	In this current paper, we now report that non-GPI-anchored CD59 is present in the cytoplasm, colocalizes with exocytotic protein vesicle-associated membrane protein 2, and completely rescues GSIS in cells lacking endogenous CD59 expression.					
31412214	1	89	theme	complex	250:256	arg1	inhibitor					206:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor	147:214	a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC)	147:262	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
31412214	1	89	theme	complex	250:256	arg1	CD59					139:142	CD59	139:142	CD59	139:142	CD59 is a glycosylphosphatidylinositol (GPI)-anchored cell surface inhibitor of the complement membrane attack complex (MAC).					
29738687	3	0	from	residue	668:674	arg1	OPS					698:700	the strain HS3-104 OPS	679:700	the strain HS3-104 OPS	679:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	1	1	theme	horse	353:357	arg1	feces					359:363	horse feces	353:363	horse feces	353:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	4	2	theme	cluster	849:855	arg1	exception					823:831	the exception	819:831	the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome	819:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	1	3	theme	Glycerophosphate-containing	148:174	arg1	OPSs					204:207	OPSs	204:207	OPSs	204:207	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	3	theme	Glycerophosphate-containing	148:174	arg1	polysaccharides					187:201	Glycerophosphate-containing O-specific polysaccharides	148:201	Glycerophosphate-containing O-specific polysaccharides (OPSs)	148:208	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	3	4	contain	had	587:589	arg1	OPSs					582:585	Both OPSs	577:585	Both OPSs	577:585	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	3	4	contain	had	587:589	arg2	structures					599:608	similar structures	591:608	similar structures	591:608	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	4	5	theme	gene	844:847	arg1	cluster					849:855	the gtr gene cluster	836:855	the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome	836:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	1	6	theme	O-specific	176:185	arg1	OPSs					204:207	OPSs	204:207	OPSs	204:207	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	6	theme	O-specific	176:185	arg1	polysaccharides					187:201	Glycerophosphate-containing O-specific polysaccharides	148:201	Glycerophosphate-containing O-specific polysaccharides (OPSs)	148:208	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	2	7	theme	one-	522:525	arg1	1H-					547:549	one- and two-dimensional 1H- and 13C-NMR spectroscopy	522:574	1H-	547:549	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	3	8	theme	strain	683:688	arg1	OPS					698:700	the strain HS3-104 OPS	679:700	the strain HS3-104 OPS	679:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	4	9	from	genome	945:950	arg1	located					920:926	located	920:926	located	920:926	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	9	from	genome	945:950	arg1	HS3-104					902:908	the strain HS3-104	891:908	the strain HS3-104	891:908	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	10	theme	biosynthesis	751:762	arg1	cluster					769:775	the O-antigen biosynthesis gene cluster	737:775	the O-antigen biosynthesis gene cluster	737:775	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	1	11	theme	Escherichia	288:298	arg1	strain					310:315	Escherichia coli type strain O81	288:319	Escherichia coli type strain O81	288:319	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	3	12	from	presence	635:642	arg1	OPS					698:700	the strain HS3-104 OPS	679:700	the strain HS3-104 OPS	679:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	4	13	theme	responsible	857:867	arg1	cluster					849:855	the gtr gene cluster	836:855	the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome	836:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	14	theme	O-antigen	741:749	arg1	cluster					769:775	the O-antigen biosynthesis gene cluster	737:775	the O-antigen biosynthesis gene cluster	737:775	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	15	from	located	920:926	arg1	genome					945:950	the genome	941:950	the genome	941:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	0	16	theme	O-Antigen	104:112	arg1	Glucosylation					114:126	O-Antigen Glucosylation	104:126	O-Antigen Glucosylation in E. coli HS3-104	104:145	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	17	from	Glucosylation	114:126	arg1	HS3-104					139:145	HS3-104	139:145	HS3-104	139:145	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	4	18	theme	cluster	769:775	arg1	identical					804:812	identical	804:812	identical	804:812	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	18	theme	cluster	769:775	arg1	genes					707:711	genes	707:711	The genes	703:711	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	18	theme	cluster	769:775	arg1	organization					721:732	the organization	717:732	the organization of the O-antigen biosynthesis gene cluster in both strains	717:791	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	2	19	theme	monosaccharide	494:507	arg1	analysis					509:516	monosaccharide analysis	494:516	monosaccharide analysis	494:516	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	4	20	theme	gene	764:767	arg1	cluster					769:775	the O-antigen biosynthesis gene cluster	737:775	the O-antigen biosynthesis gene cluster	737:775	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	21	theme	gtr	840:842	arg1	cluster					849:855	the gtr gene cluster	836:855	the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome	836:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	22	from	glucosylations	873:886	arg1	located					920:926	located	920:926	located	920:926	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	22	from	glucosylations	873:886	arg1	HS3-104					902:908	the strain HS3-104	891:908	the strain HS3-104	891:908	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	1	23	theme	type	305:308	arg1	strain					310:315	Escherichia coli type strain O81	288:319	Escherichia coli type strain O81	288:319	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	3	24	theme	glucose	660:666	arg1	residue					668:674	a side-chain glucose residue	647:674	a side-chain glucose residue in the strain HS3-104 OPS	647:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	4	25	from	organization	721:732	arg1	strains					785:791	both strains	780:791	both strains	780:791	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	3	26	theme	residue	668:674	arg1	presence					635:642	the presence	631:642	the presence of a side-chain glucose residue in the strain HS3-104 OPS	631:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	2	27	theme	OPSs	389:392	arg1	structures					370:379	The structures	366:379	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF	366:476	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	0	28	theme	coli	26:29	arg1	O81					39:41	O81	39:41	O81	39:41	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	28	theme	coli	26:29	arg1	Strains					31:37	Escherichia coli Strains O81 and HS3-104	14:53	Escherichia coli Strains O81 and HS3-104	14:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	28	theme	coli	26:29	arg1	HS3-104					47:53	HS3-104	47:53	HS3-104	47:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	29	theme	Escherichia	14:24	arg1	O81					39:41	O81	39:41	O81	39:41	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	29	theme	Escherichia	14:24	arg1	Strains					31:37	Escherichia coli Strains O81 and HS3-104	14:53	Escherichia coli Strains O81 and HS3-104	14:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	29	theme	Escherichia	14:24	arg1	HS3-104					47:53	HS3-104	47:53	HS3-104	47:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	2	30	theme	%	473:473	arg1	HF					475:476	48% HF	471:476	48% HF	471:476	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	2	31	theme	two-dimensional	531:545	arg1	1H-					547:549	one- and two-dimensional 1H- and 13C-NMR spectroscopy	522:574	1H-	547:549	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	3	32	theme	similar	591:597	arg1	structures					599:608	similar structures	591:608	similar structures	591:608	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	0	33	theme	Strains	31:37	arg1	O-Antigens					0:9	O-Antigens	0:9	O-Antigens of Escherichia coli Strains O81 and HS3-104	0:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	1	34	theme	E.	325:326	arg1	strain					333:338	E. coli strain HS3-104	325:346	E. coli strain HS3-104	325:346	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	2	35	theme	48	471:472	arg1	%					473:473	%	473:473	%	473:473	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	2	36	theme	O81	445:447	arg1	OPS					449:451	the strain O81 OPS	434:451	the strain O81 OPS	434:451	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	1	37	theme	mild	227:230	arg1	degradation					239:249	mild acidic degradation	227:249	mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces	227:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	38	theme	coli	328:331	arg1	strain					333:338	E. coli strain HS3-104	325:346	E. coli strain HS3-104	325:346	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	2	39	attach	derived	421:427	arg1	OPS					449:451	the strain O81 OPS	434:451	the strain O81 OPS	434:451	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	2	39	attach	derived	421:427	arg2	oligosaccharide					405:419	the oligosaccharide	401:419	the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF	401:476	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	3	40	from	OPS	698:700	arg1	presence					635:642	the presence	631:642	the presence of a side-chain glucose residue in the strain HS3-104 OPS	631:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	1	41	dep	Escherichia	288:298	arg1	coli					300:303	coli	300:303	coli	300:303	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	0	42	dep	Strains	31:37	arg1	O81					39:41	O81	39:41	O81	39:41	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	42	dep	Strains	31:37	arg1	Strains					31:37	Escherichia coli Strains O81 and HS3-104	14:53	Escherichia coli Strains O81 and HS3-104	14:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	0	42	dep	Strains	31:37	arg1	HS3-104					47:53	HS3-104	47:53	HS3-104	47:53	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.					
29738687	4	43	theme	strain	895:900	arg1	located					920:926	located	920:926	located	920:926	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	43	theme	strain	895:900	arg1	HS3-104					902:908	the strain HS3-104	891:908	the strain HS3-104	891:908	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	44	from	genes	707:711	arg1	strains					785:791	both strains	780:791	both strains	780:791	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	1	45	theme	acidic	232:237	arg1	degradation					239:249	mild acidic degradation	227:249	mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces	227:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	2	46	theme	13C-NMR	555:561	arg1	spectroscopy					563:574	one- and two-dimensional 1H- and 13C-NMR spectroscopy	522:574	spectroscopy	563:574	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	3	47	theme	side-chain	649:658	arg1	residue					668:674	a side-chain glucose residue	647:674	a side-chain glucose residue in the strain HS3-104 OPS	647:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	1	48	attach	isolated	274:281	arg1	feces					359:363	horse feces	353:363	horse feces	353:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	48	attach	isolated	274:281	arg1	strain					333:338	E. coli strain HS3-104	325:346	E. coli strain HS3-104	325:346	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	48	attach	isolated	274:281	arg2	lipopolysaccharides					254:272	lipopolysaccharides	254:272	lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces	254:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	1	48	attach	isolated	274:281	arg1	strain					310:315	Escherichia coli type strain O81	288:319	Escherichia coli type strain O81	288:319	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29738687	4	49	located	located	920:926	arg2	located					920:926	located	920:926	located	920:926	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	49	located	located	920:926	arg2	HS3-104					902:908	the strain HS3-104	891:908	the strain HS3-104	891:908	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	4	49	located	located	920:926	arg1	genome					945:950	the genome	941:950	the genome	941:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	2	50	theme	oligosaccharide	405:419	arg1	structures					370:379	The structures	366:379	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF	366:476	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	2	51	with	treatment	456:464	arg1	HF					475:476	48% HF	471:476	48% HF	471:476	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	4	52	with	identical	804:812	arg1	exception					823:831	the exception	819:831	the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome	819:950	The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.					
29738687	3	53	theme	HS3-104	690:696	arg1	OPS					698:700	the strain HS3-104 OPS	679:700	the strain HS3-104 OPS	679:700	Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS.					
29738687	2	54	theme	strain	438:443	arg1	OPS					449:451	the strain O81 OPS	434:451	the strain O81 OPS	434:451	The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy.					
29738687	1	55	theme	lipopolysaccharides	254:272	arg1	degradation					239:249	mild acidic degradation	227:249	mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces	227:363	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces.					
29340583	7	0	link	N-linked	1112:1119	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	9	1	theme	mass	1531:1534	arg1	spectrometry					1536:1547	tandem mass spectrometry	1524:1547	tandem mass spectrometry	1524:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	8	2	theme	insertional	1205:1215	arg1	mutants					1226:1232	insertional knockout mutants	1205:1232	insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1205:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	1	3	link	N-linked	88:95	arg1	systems					119:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	2	4	theme	single	291:296	arg1	locus					310:314	a single chromosomal locus	289:314	a single chromosomal locus	289:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	5	theme	mutants	1226:1232	arg1	background					1191:1200	the background	1187:1200	the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1187:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	8	6	link	N-linked	1383:1390	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	4	7	theme	protein	683:689	arg1	systems					705:711	N-linked protein glycosylation systems	674:711	N-linked protein glycosylation systems	674:711	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	8	8	theme	H.	1237:1238	arg1	genes					1257:1261	H. pullorum pgl/wbp genes	1237:1261	H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1237:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	6	9	theme	H.	948:949	arg1	activity					936:943	the in vitro N-linked glycosylation activity	900:943	the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	900:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	8	10	theme	structures	1299:1308	arg1	analysis					1275:1282	analysis	1275:1282	analysis of HgpA glycan structures	1275:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	10	11	link	N-linked	1769:1776	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	8	12	theme	pgl/wbp	1249:1255	arg1	genes					1257:1261	H. pullorum pgl/wbp genes	1237:1261	H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1237:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	7	13	theme	N-linked	1112:1119	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	9	14	theme	pullorum	1588:1595	arg1	proteins					1597:1604	five H. pullorum proteins	1580:1604	five H. pullorum proteins	1580:1604	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	9	15	theme	glycosylated	1697:1708	arg1	HgpA					1710:1713	glycosylated HgpA	1697:1713	glycosylated HgpA	1697:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	4	16	from	species	471:477	arg1	genomes					458:464	all genomes	454:464	all genomes from species of the Campylobacter genus	454:504	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	17	dep	H.	607:608	arg1	pullorum					610:617	H. pullorum	607:617	H. pullorum	607:617	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	8	18	theme	gene	1346:1349	arg1	products					1351:1358	individual gene products	1335:1358	individual gene products	1335:1358	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	5	19	theme	Helicobacter	714:725	arg1	genes					740:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	4	20	dep	H.	638:639	arg1	winghamensis					641:652	H. winghamensis	638:652	H. winghamensis	638:652	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	2	21	theme	Campylobacter	233:245	arg1	jejuni					247:252	Campylobacter jejuni	233:252	Campylobacter jejuni	233:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	22	from	Production	1148:1157	arg1	background					1191:1200	the background	1187:1200	the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1187:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	5	23	theme	pgl	736:738	arg1	genes					740:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	2	24	attach	present	278:284	arg2	genes					272:276	pgl genes	268:276	pgl genes present at a single chromosomal locus	268:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	2	24	attach	present	278:284	arg1	locus					310:314	a single chromosomal locus	289:314	a single chromosomal locus	289:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	25	theme	PglB1-dependent	1367:1381	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	0	26	theme	glycosylation	66:78	arg1	system					80:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	3	27	from	carrier	425:431	arg1	transfer					395:402	transfer	395:402	transfer of glycan from lipid carrier to protein	395:442	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	9	28	theme	reactive	1642:1649	arg1	proteins					1633:1640	proteins	1633:1640	proteins reactive with a polyclonal antiserum generated against glycosylated HgpA	1633:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	8	29	theme	protein	1392:1398	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	5	30	theme	oligosaccharyltransferase-encoding	810:843	arg1	genes					850:854	two putative oligosaccharyltransferase-encoding pglB genes	797:854	two putative oligosaccharyltransferase-encoding pglB genes per genome	797:865	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	1	31	theme	life	172:175	arg1	domains					161:167	all three domains	151:167	all three domains of life	151:175	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	1	31	theme	life	172:175	arg1	life					172:175	life	172:175	life	172:175	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	5	32	theme	chromosomal	768:778	arg1	loci					780:783	five chromosomal loci	763:783	five chromosomal loci	763:783	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	4	33	dep	species	598:604	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	33	dep	species	598:604	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	33	dep	species	598:604	arg1	H.					607:608	H.	607:608	H.	607:608	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	33	dep	species	598:604	arg1	H.					638:639	H.	638:639	H.	638:639	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	2	34	from	jejuni	247:252	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	4	35	theme	genus	500:504	arg1	species					471:477	species	471:477	species of the Campylobacter genus	471:504	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	9	36	with	consistent	1617:1626	arg1	proteins					1633:1640	proteins	1633:1640	proteins reactive with a polyclonal antiserum generated against glycosylated HgpA	1633:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	1	37	theme	N-linked	88:95	arg1	systems					119:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	4	38	theme	related	590:596	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	38	theme	related	590:596	arg1	H.					638:639	H.	638:639	H.	638:639	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	38	theme	related	590:596	arg1	H.					607:608	H.	607:608	H.	607:608	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	38	theme	related	590:596	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	10	39	theme	protein	1786:1792	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	0	40	theme	Functional	0:9	arg1	analysis					11:18	Functional analysis	0:18	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.	0:86	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	1	41	theme	glycosylation	105:117	arg1	systems					119:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	6	42	dep	in	904:905	arg1	vitro					907:911	vitro	907:911	vitro	907:911	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	2	43	theme	model	182:186	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	44	theme	HgpA	1287:1290	arg1	structures					1299:1308	HgpA glycan structures	1287:1308	HgpA glycan structures	1287:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	0	45	theme	Helicobacter	27:38	arg1	system					80:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	6	46	theme	glycosylation	922:934	arg1	activity					936:943	the in vitro N-linked glycosylation activity	900:943	the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	900:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	9	47	from	proteins	1597:1604	arg1	glycosites					1564:1573	six glycosites	1560:1573	six glycosites	1560:1573	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	4	48	theme	related	537:543	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	48	theme	related	537:543	arg1	H.					638:639	H.	638:639	H.	638:639	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	48	theme	related	537:543	arg1	H.					607:608	H.	607:608	H.	607:608	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	48	theme	related	537:543	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	9	49	theme	zwitterionic-hydrophilic	1452:1475	arg1	chromatography					1496:1509	zwitterionic-hydrophilic interaction liquid chromatography	1452:1509	zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry	1452:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	0	50	dep	Helicobacter	27:38	arg1	pullorum					40:47	pullorum	40:47	pullorum	40:47	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	0	50	dep	Helicobacter	27:38	arg1	N-linked					49:56	N-linked	49:56	N-linked	49:56	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	4	51	theme	genus	558:562	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	51	theme	genus	558:562	arg1	H.					638:639	H.	638:639	H.	638:639	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	51	theme	genus	558:562	arg1	H.					607:608	H.	607:608	H.	607:608	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	51	theme	genus	558:562	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	8	52	dep	H.	1237:1238	arg1	pullorum					1240:1247	H. pullorum	1237:1247	H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1237:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	9	53	theme	liquid	1489:1494	arg1	chromatography					1496:1509	zwitterionic-hydrophilic interaction liquid chromatography	1452:1509	zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry	1452:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	6	54	dep	H.	948:949	arg1	pullorum					951:958	H. pullorum	948:958	H. pullorum	948:958	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	2	55	theme	N-linked	198:205	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	56	theme	HgpA	1179:1182	arg1	Production					1148:1157	Production	1148:1157	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1148:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	6	57	theme	pentasaccharide	987:1001	arg1	transfer					973:980	transfer	973:980	transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	973:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	9	58	gly	glycosylated	1697:1708	arg1	HgpA					1710:1713	glycosylated HgpA	1697:1713	glycosylated HgpA	1697:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	10	59	dep	H.	1757:1758	arg1	pullorum					1760:1767	H. pullorum	1757:1767	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	9	60	theme	tandem	1524:1529	arg1	spectrometry					1536:1547	tandem mass spectrometry	1524:1547	tandem mass spectrometry	1524:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	2	61	theme	chromosomal	298:308	arg1	locus					310:314	a single chromosomal locus	289:314	a single chromosomal locus	289:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	62	theme	knockout	1217:1224	arg1	mutants					1226:1232	insertional knockout mutants	1205:1232	insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1205:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	4	63	theme	N-linked	674:681	arg1	systems					705:711	N-linked protein glycosylation systems	674:711	N-linked protein glycosylation systems	674:711	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	6	64	theme	sequon	1041:1046	arg1	XNXS/T					1053:1058	the sequon (D/E)XNXS/T	1037:1058	the sequon (D/E)XNXS/T	1037:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	7	65	gly	glycoprotein	1121:1132	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	7	66	theme	pullorum	1103:1110	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	9	67	theme	H.	1585:1586	arg1	proteins					1597:1604	five H. pullorum proteins	1580:1604	five H. pullorum proteins	1580:1604	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	9	68	gly	glycosites	1564:1573	arg2	glycosites					1564:1573	six glycosites	1560:1573	six glycosites	1560:1573	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	10	69	theme	N-linked	1769:1776	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	7	70	theme	first	1094:1098	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	4	71	theme	glycosylation	691:703	arg1	systems					705:711	N-linked protein glycosylation systems	674:711	N-linked protein glycosylation systems	674:711	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	8	72	theme	genes	1257:1261	arg1	mutants					1226:1232	insertional knockout mutants	1205:1232	insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures	1205:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	4	73	theme	Campylobacter	486:498	arg1	genus					500:504	the Campylobacter genus	482:504	the Campylobacter genus	482:504	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	10	74	theme	H.	1757:1758	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	3	75	theme	gene	322:325	arg1	cluster					327:333	This gene cluster	317:333	This gene cluster	317:333	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	4	76	link	N-linked	674:681	arg1	systems					705:711	N-linked protein glycosylation systems	674:711	N-linked protein glycosylation systems	674:711	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	8	77	theme	individual	1335:1344	arg1	products					1351:1358	individual gene products	1335:1358	individual gene products	1335:1358	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	5	78	theme	putative	727:734	arg1	genes					740:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes	714:744	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	2	79	theme	present	278:284	arg1	genes					272:276	pgl genes	268:276	pgl genes present at a single chromosomal locus	268:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	80	theme	products	1351:1358	arg1	role					1327:1330	the role	1323:1330	the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway	1323:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	0	81	theme	system	80:85	arg1	analysis					11:18	Functional analysis	0:18	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.	0:86	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	2	82	theme	pgl	268:270	arg1	genes					272:276	pgl genes	268:276	pgl genes present at a single chromosomal locus	268:314	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	83	from	role	1327:1330	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	3	84	theme	pglB	348:351	arg1	oligosaccharyltransferase					353:377	the pglB oligosaccharyltransferase	344:377	the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein	344:442	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	5	85	theme	putative	801:808	arg1	genes					850:854	two putative oligosaccharyltransferase-encoding pglB genes	797:854	two putative oligosaccharyltransferase-encoding pglB genes per genome	797:865	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	8	86	theme	glycan	1292:1297	arg1	structures					1299:1308	HgpA glycan structures	1287:1308	HgpA glycan structures	1287:1308	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	2	87	from	locus	310:314	arg1	present					278:284	present	278:284	present	278:284	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	8	88	theme	N-linked	1383:1390	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	9	89	theme	polyclonal	1658:1667	arg1	antiserum					1669:1677	a polyclonal antiserum	1656:1677	a polyclonal antiserum generated against glycosylated HgpA	1656:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	6	90	theme	D/E	1049:1051	arg1	XNXS/T					1053:1058	the sequon (D/E)XNXS/T	1037:1058	the sequon (D/E)XNXS/T	1037:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	9	91	with	reactive	1642:1649	arg1	antiserum					1669:1677	a polyclonal antiserum	1656:1677	a polyclonal antiserum generated against glycosylated HgpA	1656:1713	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	8	92	theme	glycosylation	1400:1412	arg1	pathway					1414:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	the PglB1-dependent N-linked protein glycosylation pathway	1363:1420	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	1	93	from	domains	161:167	arg1	species					138:144	species	138:144	species from all three domains of life	138:175	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	3	94	theme	responsible	379:389	arg1	oligosaccharyltransferase					353:377	the pglB oligosaccharyltransferase	344:377	the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein	344:442	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	4	95	theme	pgl	516:518	arg1	locus					520:524	a pgl locus	514:524	a pgl locus	514:524	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	96	theme	H.	620:621	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	96	theme	H.	620:621	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	10	97	theme	glycosylation	1794:1806	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	1	98	theme	protein	97:103	arg1	systems					119:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems	88:125	N-linked protein glycosylation systems operate in species from all three domains of life.					
29340583	3	99	theme	lipid	419:423	arg1	carrier					425:431	lipid carrier	419:431	lipid carrier to protein	419:442	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	10	100	theme	general	1778:1784	arg1	system					1808:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	a H. pullorum N-linked general protein glycosylation system	1755:1813	This study demonstrates functioning of a H. pullorum N-linked general protein glycosylation system.					
29340583	3	101	theme	glycan	407:412	arg1	transfer					395:402	transfer	395:402	transfer of glycan from lipid carrier to protein	395:442	This gene cluster includes the pglB oligosaccharyltransferase responsible for transfer of glycan from lipid carrier to protein.					
29340583	6	102	from	asparagine	1019:1028	arg1	peptide					1008:1014	a peptide	1006:1014	a peptide at asparagine within the sequon (D/E)XNXS/T	1006:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	2	103	theme	bacterial	188:196	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	2	104	link	N-linked	198:205	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	5	105	theme	pglB	845:848	arg1	genes					850:854	two putative oligosaccharyltransferase-encoding pglB genes	797:854	two putative oligosaccharyltransferase-encoding pglB genes per genome	797:865	Helicobacter putative pgl genes are scattered in five chromosomal loci and include two putative oligosaccharyltransferase-encoding pglB genes per genome.					
29340583	9	106	theme	Glycopeptide	1423:1434	arg1	purification					1436:1447	Glycopeptide purification	1423:1447	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry	1423:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	4	107	theme	Helicobacter	545:556	arg1	canadensis					623:632	H. canadensis	620:632	H. canadensis	620:632	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	107	theme	Helicobacter	545:556	arg1	H.					638:639	H.	638:639	H.	638:639	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	107	theme	Helicobacter	545:556	arg1	H.					607:608	H.	607:608	H.	607:608	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	107	theme	Helicobacter	545:556	arg1	species					598:604	the related Helicobacter genus only three evolutionarily related species	533:604	the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis)	533:653	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	0	108	theme	protein	58:64	arg1	system					80:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	the Helicobacter pullorum N-linked protein glycosylation system	23:85	Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system.					
29340583	6	109	theme	N-linked	913:920	arg1	glycosylation					922:934	the in vitro N-linked glycosylation	900:934	the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	900:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	7	110	theme	H.	1100:1101	arg1	glycoprotein					1121:1132	the first H. pullorum N-linked glycoprotein	1090:1132	the first H. pullorum N-linked glycoprotein	1090:1132	In this study, we identified the first H. pullorum N-linked glycoprotein, termed HgpA.					
29340583	6	111	link	N-linked	913:920	arg1	glycosylation					922:934	the in vitro N-linked glycosylation	900:934	the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	900:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	9	112	theme	interaction	1477:1487	arg1	chromatography					1496:1509	zwitterionic-hydrophilic interaction liquid chromatography	1452:1509	zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry	1452:1547	Glycopeptide purification by zwitterionic-hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry identified six glycosites from five H. pullorum proteins, which was consistent with proteins reactive with a polyclonal antiserum generated against glycosylated HgpA.					
29340583	8	113	theme	histidine-tagged	1162:1177	arg1	HgpA					1179:1182	histidine-tagged HgpA	1162:1182	histidine-tagged HgpA	1162:1182	Production of histidine-tagged HgpA in the background of insertional knockout mutants of H. pullorum pgl/wbp genes followed by analysis of HgpA glycan structures demonstrated the role of individual gene products in the PglB1-dependent N-linked protein glycosylation pathway.					
29340583	6	114	theme	in	904:905	arg1	glycosylation					922:934	the in vitro N-linked glycosylation	900:934	the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T	900:1058	We have previously demonstrated the in vitro N-linked glycosylation activity of H. pullorum resulting in transfer of a pentasaccharide to a peptide at asparagine within the sequon (D/E)XNXS/T.					
29340583	2	115	theme	glycosylation	207:219	arg1	system					221:226	The model bacterial N-linked glycosylation system	178:226	The model bacterial N-linked glycosylation system from Campylobacter jejuni	178:252	The model bacterial N-linked glycosylation system from Campylobacter jejuni is encoded by pgl genes present at a single chromosomal locus.					
29340583	4	116	contain	contain	506:512	arg1	genomes					458:464	all genomes	454:464	all genomes from species of the Campylobacter genus	454:504	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
29340583	4	116	contain	contain	506:512	arg2	locus					520:524	a pgl locus	514:524	a pgl locus	514:524	Although all genomes from species of the Campylobacter genus contain a pgl locus, among the related Helicobacter genus only three evolutionarily related species (H. pullorum, H. canadensis and H. winghamensis) potentially encode N-linked protein glycosylation systems.					
31311973	4	0	theme	N-linked	777:784	arg1	glycans					786:792	the N-linked glycans	773:792	the N-linked glycans at the interface of GluK3 ATD and LBD	773:830	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	6	1	link	N-linked	1112:1119	arg1	glycosylation					1121:1133	N-linked glycosylation	1112:1133	N-linked glycosylation	1112:1133	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	4	2	from	interface	801:809	arg1	glycans					786:792	the N-linked glycans	773:792	the N-linked glycans at the interface of GluK3 ATD and LBD	773:830	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	2	3	theme	GluK3	352:356	arg1	receptors					358:366	GluK3 receptors	352:366	GluK3 receptors	352:366	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	1	4	theme	synaptic	246:253	arg1	transmission					255:266	synaptic transmission	246:266	synaptic transmission	246:266	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	3	5	theme	kainate	576:582	arg1	receptor-GluK2					584:597	another kainate receptor-GluK2	568:597	another kainate receptor-GluK2	568:597	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	1	6	theme	transmission	255:266	arg1	regulation					232:241	the regulation	228:241	the regulation of synaptic transmission	228:266	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	2	7	theme	better	275:280	arg1	understanding					282:294	a better understanding	273:294	a better understanding of structural changes that underlie receptor functions	273:349	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	0	8	theme	Structural	0:9	arg1	Insights					26:33	Structural and Functional Insights	0:33	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery	0:90	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.					
31311973	6	9	theme	gating	1052:1057	arg1	properties					1059:1068	gating properties	1052:1068	gating properties unique to GluK3	1052:1084	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	4	10	dep	GluK3	814:818	arg1	ATD					820:822	ATD	820:822	ATD	820:822	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	4	10	dep	GluK3	814:818	arg1	LBD					828:830	LBD	828:830	LBD	828:830	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	2	11	theme	resting/closed	401:414	arg1	states					416:421	states	416:421	states	416:421	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	3	12	with	state	641:645	arg1	domains					665:671	only two LBD domains	652:671	only two LBD domains	652:671	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	5	13	theme	putative	952:959	arg1	residues					982:989	putative N-glycan interacting residues	952:989	putative N-glycan interacting residues	952:989	The mutational analysis also identified putative N-glycan interacting residues.					
31311973	1	14	theme	GluK3-kainate	93:105	arg1	receptors					107:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	1	14	theme	GluK3-kainate	93:105	arg1	members					130:136	atypical members	121:136	atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission	121:266	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	6	15	theme	molecular	1014:1022	arg1	framework					1024:1032	a molecular framework	1012:1032	a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation	1012:1153	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	2	16	theme	cryo-electron	469:481	arg1	microscopy					483:492	single particle cryo-electron microscopy	453:492	single particle cryo-electron microscopy	453:492	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	3	17	contain	has	524:526	arg1	GluK3					518:522	the desensitized GluK3	501:522	the desensitized GluK3	501:522	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	3	17	contain	has	524:526	arg2	organization					535:546	domain organization	528:546	domain organization	528:546	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	4	18	theme	receptor-gating	884:898	arg1	properties					900:909	receptor-gating properties	884:909	receptor-gating properties	884:909	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	6	19	from	role	1104:1107	arg1	modulation					1144:1153	their modulation	1138:1153	their modulation	1138:1153	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	3	20	theme	bound	611:615	arg1	GluK3					617:621	antagonist bound GluK3	600:621	antagonist bound GluK3	600:621	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	2	21	theme	desensitized	384:395	arg1	states					416:421	states	416:421	states	416:421	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	2	22	theme	particle	460:467	arg1	microscopy					483:492	single particle cryo-electron microscopy	453:492	single particle cryo-electron microscopy	453:492	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	2	23	theme	changes	310:316	arg1	understanding					282:294	a better understanding	273:294	a better understanding of structural changes that underlie receptor functions	273:349	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	5	24	theme	mutational	916:925	arg1	analysis					927:934	The mutational analysis	912:934	The mutational analysis	912:934	The mutational analysis also identified putative N-glycan interacting residues.					
31311973	2	25	theme	single	453:458	arg1	microscopy					483:492	single particle cryo-electron microscopy	453:492	single particle cryo-electron microscopy	453:492	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	2	26	theme	structural	299:308	arg1	changes					310:316	structural changes	299:316	structural changes that underlie receptor functions	299:349	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	6	27	theme	glycosylation	1121:1133	arg1	role					1104:1107	the role	1100:1107	the role of N-linked glycosylation in their modulation	1100:1153	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	0	28	theme	Functional	15:24	arg1	Insights					26:33	Structural and Functional Insights	0:33	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery	0:90	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.					
31311973	3	29	theme	dimeric	676:682	arg1	arrangement					684:694	dimeric arrangement	676:694	dimeric arrangement necessary for receptor activation	676:728	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	3	30	theme	antagonist	600:609	arg1	GluK3					617:621	antagonist bound GluK3	600:621	antagonist bound GluK3	600:621	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	6	31	theme	N-linked	1112:1119	arg1	glycosylation					1121:1133	N-linked glycosylation	1112:1133	N-linked glycosylation	1112:1133	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	0	32	theme	GluK3-kainate	40:52	arg1	Desensitization					63:77	GluK3-kainate Receptor Desensitization	40:77	GluK3-kainate Receptor Desensitization	40:77	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.					
31311973	5	33	theme	interacting	970:980	arg1	residues					982:989	putative N-glycan interacting residues	952:989	putative N-glycan interacting residues	952:989	The mutational analysis also identified putative N-glycan interacting residues.					
31311973	3	34	theme	resting	633:639	arg1	state					641:645	a resting state	631:645	a resting state with only two LBD domains	631:671	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	6	35	theme	unique	1070:1075	arg1	properties					1059:1068	gating properties	1052:1068	gating properties unique to GluK3	1052:1084	Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.					
31311973	1	36	theme	vital	214:218	arg1	role					220:223	a vital role	212:223	a vital role	212:223	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	4	37	theme	inter-domain	847:858	arg1	interactions					860:871	inter-domain interactions	847:871	inter-domain interactions	847:871	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	5	38	theme	N-glycan	961:968	arg1	residues					982:989	putative N-glycan interacting residues	952:989	putative N-glycan interacting residues	952:989	The mutational analysis also identified putative N-glycan interacting residues.					
31311973	0	39	theme	Receptor	54:61	arg1	Desensitization					63:77	GluK3-kainate Receptor Desensitization	40:77	GluK3-kainate Receptor Desensitization	40:77	Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.					
31311973	3	40	theme	desensitized	505:516	arg1	GluK3					518:522	the desensitized GluK3	501:522	the desensitized GluK3	501:522	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	3	41	theme	receptor	710:717	arg1	activation					719:728	receptor activation	710:728	receptor activation	710:728	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	1	42	dep	pre-	182:185	arg1	the					178:180	the	178:180	the	178:180	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	3	43	theme	domain	528:533	arg1	organization					535:546	domain organization	528:546	domain organization	528:546	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	4	44	theme	GluK3	814:818	arg1	interface					801:809	the interface	797:809	the interface of GluK3 ATD and LBD	797:830	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
31311973	1	45	theme	iGluR	145:149	arg1	family					151:156	the iGluR family	141:156	the iGluR family	141:156	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	1	46	theme	atypical	121:128	arg1	receptors					107:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	1	46	theme	atypical	121:128	arg1	members					130:136	atypical members	121:136	atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission	121:266	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	3	47	theme	necessary	696:704	arg1	arrangement					684:694	dimeric arrangement	676:694	dimeric arrangement necessary for receptor activation	676:728	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	3	48	theme	LBD	661:663	arg1	domains					665:671	only two LBD domains	652:671	only two LBD domains	652:671	While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation.					
31311973	1	49	theme	family	151:156	arg1	receptors					107:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors	93:115	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	1	49	theme	family	151:156	arg1	members					130:136	atypical members	121:136	atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission	121:266	GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission.					
31311973	2	50	theme	receptor	332:339	arg1	functions					341:349	receptor functions	332:349	receptor functions	332:349	For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy.					
31311973	4	51	link	N-linked	777:784	arg1	glycans					786:792	the N-linked glycans	773:792	the N-linked glycans at the interface of GluK3 ATD and LBD	773:830	Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties.					
29775210	6	0	theme	fluorescent	1052:1062	arg1	detection					1064:1072	laser induced fluorescent detection	1038:1072	laser induced fluorescent detection	1038:1072	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	3	1	theme	chronic	484:490	arg1	inflammation					492:503	chronic inflammation	484:503	chronic inflammation	484:503	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	6	2	theme	samples	1156:1162	arg1	N-glycosylation					1131:1145	N-glycosylation	1131:1145	N-glycosylation of hIgG1 samples	1131:1162	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	2	3	theme	radiation	244:252	arg1	dose					254:257	This elevated radiation dose	230:257	This elevated radiation dose	230:257	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	5	4	dep	solution	962:969	arg1	form					971:974	form	971:974	form	971:974	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	2	5	theme	Galactic	276:283	arg1	Rays					292:295	Galactic Cosmic Rays	276:295	Galactic Cosmic Rays	276:295	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	8	6	gly	carbohydrate	1440:1451	arg1	IgG1					1464:1467	IgG1	1464:1467	IgG1	1464:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	6	7	theme	space	1112:1116	arg1	radiation					1118:1126	simulated space radiation	1102:1126	simulated space radiation	1102:1126	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	6	8	theme	induced	1044:1050	arg1	detection					1064:1072	laser induced fluorescent detection	1038:1072	laser induced fluorescent detection	1038:1072	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	1	9	theme	radiation	177:185	arg1	radiation					177:185	radiation	177:185	radiation	177:185	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	1	9	theme	radiation	177:185	arg1	amount					167:172	an approximate amount	152:172	an approximate amount of radiation that exceeds the lifetime limits on Earth	152:227	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	4	10	theme	IgG	610:612	arg1	profile					630:636	the IgG N-glycosylation profile	606:636	the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation	606:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	8	11	theme	N-linked	1431:1438	arg1	profile					1453:1459	N-linked carbohydrate profile	1431:1459	N-linked carbohydrate profile of IgG1	1431:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	7	12	theme	Orbit	1348:1352	arg1	environment					1323:1333	the shielding environment	1309:1333	the shielding environment of Low Earth Orbit	1309:1352	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	5	13	theme	hIgG	873:876	arg1	samples					878:884	solidified hIgG samples	862:884	solidified hIgG samples in vacuum	862:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	7	14	theme	Low	1338:1340	arg1	Orbit					1348:1352	Low Earth Orbit	1338:1352	Low Earth Orbit	1338:1352	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	8	15	theme	IgG1	1464:1467	arg1	profile					1453:1459	N-linked carbohydrate profile	1431:1459	N-linked carbohydrate profile of IgG1	1431:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	7	16	theme	thousand	1204:1211	arg1	times					1213:1217	even several thousand times	1191:1217	even several thousand times	1191:1217	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	4	17	theme	associated	663:672	arg1	changes					674:680	disease associated changes	655:680	disease associated changes	655:680	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	0	18	theme	human	66:70	arg1	G1					87:88	human immunoglobulin G1	66:88	human immunoglobulin G1	66:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	5	19	from	irradiation	847:857	arg1	vacuum					889:894	vacuum	889:894	vacuum	889:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	3	20	theme	Specific	324:331	arg1	patterns					333:340	Specific patterns	324:340	Specific patterns of the N-glycosylation of human Igs	324:376	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	0	21	theme	G1	87:88	arg1	N-glycosylation					47:61	the N-glycosylation	43:61	the N-glycosylation of human immunoglobulin G1	43:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	8	22	theme	diagnostic	1494:1503	arg1	tools					1505:1509	molecular diagnostic tools	1484:1509	molecular diagnostic tools in space	1484:1518	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	8	22	theme	diagnostic	1494:1503	arg1	changes					1420:1426	changes	1420:1426	changes in N-linked carbohydrate profile of IgG1	1420:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	4	23	theme	space	739:743	arg1	radiation					745:753	space radiation	739:753	space radiation	739:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	3	24	gly	N-glycosylation	349:363	arg1	Igs					374:376	human Igs	368:376	human Igs	368:376	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	5	25	from	samples	878:884	arg1	vacuum					889:894	vacuum	889:894	vacuum	889:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	7	26	theme	shielding	1313:1321	arg1	environment					1323:1333	the shielding environment	1309:1333	the shielding environment of Low Earth Orbit	1309:1352	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	3	27	theme	N-glycosylation	349:363	arg1	patterns					333:340	Specific patterns	324:340	Specific patterns of the N-glycosylation of human Igs	324:376	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	4	28	theme	space	707:711	arg1	travel					713:718	space travel	707:718	space travel	707:718	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	7	29	located	observed	1371:1378	arg2	changes					1358:1364	no changes	1355:1364	no changes	1355:1364	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	7	29	located	observed	1371:1378	arg1	N-glycosylation					1389:1403	hIgG1 N-glycosylation	1383:1403	hIgG1 N-glycosylation	1383:1403	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	7	30	dep	doses	1236:1240	arg1	that					1242:1245	that	1242:1245	that	1242:1245	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	7	31	theme	duration	1284:1291	arg1	missions					1293:1300	long duration missions	1279:1300	long duration missions	1279:1300	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	0	32	theme	space	24:28	arg1	radiation					30:38	simulated space radiation	14:38	simulated space radiation	14:38	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	6	33	theme	carbohydrate	988:999	arg1	analysis					1001:1008	Structural carbohydrate analysis	977:1008	Structural carbohydrate analysis	977:1008	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	3	34	theme	human	368:372	arg1	Igs					374:376	human Igs	368:376	human Igs	368:376	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	5	35	dep	Van	815:817	arg1	de					819:820	Van de	815:820	a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum	813:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	3	36	theme	malignant	458:466	arg1	transformation					468:481	malignant transformation	458:481	malignant transformation	458:481	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	5	37	theme	ionization	760:769	arg1	sources					771:777	Two ionization sources	756:777	Two ionization sources	756:777	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	5	38	used	used	784:787	arg2	sources					771:777	Two ionization sources	756:777	Two ionization sources	756:777	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	6	39	from	effects	1091:1097	arg1	N-glycosylation					1131:1145	N-glycosylation	1131:1145	N-glycosylation of hIgG1 samples	1131:1162	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	5	40	theme	aqueous	954:960	arg1	solution					962:969	aqueous solution form	954:974	aqueous solution form	954:974	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	4	41	theme	low-energy	573:582	arg1	irradiation					591:601	low-energy proton irradiation	573:601	low-energy proton irradiation	573:601	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	7	42	theme	higher	1219:1224	arg1	doses					1236:1240	even several thousand times higher radiation doses	1191:1240	even several thousand times higher radiation doses that of astronauts	1191:1259	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	6	43	theme	hIgG1	1150:1154	arg1	samples					1156:1162	hIgG1 samples	1150:1162	hIgG1 samples	1150:1162	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	2	44	theme	Particle	307:314	arg1	Events					316:321	Solar Particle Events	301:321	Solar Particle Events	301:321	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	1	45	theme	approximate	155:165	arg1	radiation					177:185	radiation	177:185	radiation	177:185	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	1	45	theme	approximate	155:165	arg1	amount					167:172	an approximate amount	152:172	an approximate amount of radiation that exceeds the lifetime limits on Earth	152:227	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	5	46	theme	Van	815:817	arg1	generator					829:837	a Van de Graaff generator	813:837	a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum	813:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	4	47	theme	N-glycosylation	614:628	arg1	profile					630:636	the IgG N-glycosylation profile	606:636	the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation	606:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	4	48	from	effect	563:568	arg1	profile					630:636	the IgG N-glycosylation profile	606:636	the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation	606:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	2	49	theme	Cosmic	285:290	arg1	Rays					292:295	Galactic Cosmic Rays	276:295	Galactic Cosmic Rays	276:295	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	5	50	theme	Graaff	822:827	arg1	generator					829:837	a Van de Graaff generator	813:837	a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum	813:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	8	51	theme	molecular	1484:1492	arg1	tools					1505:1509	molecular diagnostic tools	1484:1509	molecular diagnostic tools in space	1484:1518	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	8	51	theme	molecular	1484:1492	arg1	changes					1420:1426	changes	1420:1426	changes in N-linked carbohydrate profile of IgG1	1420:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	7	52	theme	hIgG1	1383:1387	arg1	N-glycosylation					1389:1403	hIgG1 N-glycosylation	1383:1403	hIgG1 N-glycosylation	1383:1403	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	8	53	from	changes	1420:1426	arg1	profile					1453:1459	N-linked carbohydrate profile	1431:1459	N-linked carbohydrate profile of IgG1	1431:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	5	54	theme	Tandetron	903:911	arg1	accelerator					913:923	a Tandetron accelerator	901:923	a Tandetron accelerator	901:923	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	6	55	theme	radiation	1118:1126	arg1	effects					1091:1097	the effects	1087:1097	the effects of simulated space radiation on N-glycosylation of hIgG1 samples	1087:1162	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	6	56	theme	simulated	1102:1110	arg1	radiation					1118:1126	simulated space radiation	1102:1126	simulated space radiation	1102:1126	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	4	57	theme	irradiation	591:601	arg1	effect					563:568	the effect	559:568	the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation	559:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	8	58	theme	carbohydrate	1440:1451	arg1	profile					1453:1459	N-linked carbohydrate profile	1431:1459	N-linked carbohydrate profile of IgG1	1431:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	6	59	gly	N-glycosylation	1131:1145	arg1	samples					1156:1162	hIgG1 samples	1150:1162	hIgG1 samples	1150:1162	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	1	60	from	limits	213:218	arg1	Earth					223:227	Earth	223:227	Earth	223:227	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	5	61	theme	solidified	862:871	arg1	samples					878:884	solidified hIgG samples	862:884	solidified hIgG samples in vacuum	862:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	2	62	theme	elevated	235:242	arg1	dose					254:257	This elevated radiation dose	230:257	This elevated radiation dose	230:257	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	5	63	theme	samples	878:884	arg1	irradiation					847:857	the irradiation	843:857	the irradiation of solidified hIgG samples in vacuum	843:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	6	64	theme	Structural	977:986	arg1	analysis					1001:1008	Structural carbohydrate analysis	977:1008	Structural carbohydrate analysis	977:1008	Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples.					
29775210	7	65	theme	Earth	1342:1346	arg1	Orbit					1348:1352	Low Earth Orbit	1338:1352	Low Earth Orbit	1338:1352	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	7	66	theme	several	1196:1202	arg1	times					1213:1217	even several thousand times	1191:1217	even several thousand times	1191:1217	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	8	67	used	used	1476:1479	arg2	changes					1420:1426	changes	1420:1426	changes in N-linked carbohydrate profile of IgG1	1420:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	8	67	used	used	1476:1479	arg2	tools					1505:1509	molecular diagnostic tools	1484:1509	molecular diagnostic tools in space	1484:1518	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	0	68	theme	immunoglobulin	72:85	arg1	G1					87:88	human immunoglobulin G1	66:88	human immunoglobulin G1	66:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	0	69	from	effect	4:9	arg1	N-glycosylation					47:61	the N-glycosylation	43:61	the N-glycosylation of human immunoglobulin G1	43:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	4	70	theme	disease	655:661	arg1	changes					674:680	disease associated changes	655:680	disease associated changes	655:680	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	0	71	gly	N-glycosylation	47:61	arg1	G1					87:88	human immunoglobulin G1	66:88	human immunoglobulin G1	66:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	3	72	theme	various	412:418	arg1	diseases					448:455	autoimmune diseases	437:455	autoimmune diseases	437:455	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	3	72	theme	various	412:418	arg1	transformation					468:481	malignant transformation	458:481	malignant transformation	458:481	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	3	72	theme	various	412:418	arg1	inflammation					492:503	chronic inflammation	484:503	chronic inflammation	484:503	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	3	72	theme	various	412:418	arg1	ageing					510:515	ageing	510:515	ageing	510:515	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	3	72	theme	various	412:418	arg1	ailments					420:427	various ailments	412:427	various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing	412:515	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	0	73	theme	simulated	14:22	arg1	radiation					30:38	simulated space radiation	14:38	simulated space radiation	14:38	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	7	74	theme	long	1279:1282	arg1	missions					1293:1300	long duration missions	1279:1300	long duration missions	1279:1300	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	4	75	theme	work	535:538	arg1	focus					522:526	The focus	518:526	The focus of our work	518:538	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	3	76	theme	autoimmune	437:446	arg1	diseases					448:455	autoimmune diseases	437:455	autoimmune diseases	437:455	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	0	77	theme	radiation	30:38	arg1	effect					4:9	The effect	0:9	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1	0:88	The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1.					
29775210	4	78	with	profile	630:636	arg1	goal					647:650	the goal if disease associated changes could be detected during space travel and not altered by space radiation	643:753	the goal if disease associated changes could be detected during space travel and not altered by space radiation	643:753	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29775210	8	79	from	tools	1505:1509	arg1	space					1514:1518	space	1514:1518	space	1514:1518	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	7	80	theme	astronauts	1250:1259	arg1	doses					1236:1240	even several thousand times higher radiation doses	1191:1240	even several thousand times higher radiation doses that of astronauts	1191:1259	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	3	81	theme	Igs	374:376	arg1	N-glycosylation					349:363	the N-glycosylation	345:363	the N-glycosylation of human Igs	345:376	Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing.					
29775210	2	82	theme	Solar	301:305	arg1	Events					316:321	Solar Particle Events	301:321	Solar Particle Events	301:321	This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events.					
29775210	5	83	theme	hIgG	938:941	arg1	samples					943:949	hIgG samples	938:949	hIgG samples	938:949	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	5	84	from	vacuum	889:894	arg1	irradiation					847:857	the irradiation	843:857	the irradiation of solidified hIgG samples in vacuum	843:894	Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form.					
29775210	1	85	theme	lifetime	204:211	arg1	limits					213:218	the lifetime limits	200:218	the lifetime limits on Earth	200:227	On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth.					
29775210	7	86	theme	radiation	1226:1234	arg1	doses					1236:1240	even several thousand times higher radiation doses	1191:1240	even several thousand times higher radiation doses that of astronauts	1191:1259	Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of Low Earth Orbit, no changes were observed in hIgG1 N-glycosylation.					
29775210	8	87	link	N-linked	1431:1438	arg1	profile					1453:1459	N-linked carbohydrate profile	1431:1459	N-linked carbohydrate profile of IgG1	1431:1467	Consequently, changes in N-linked carbohydrate profile of IgG1 can be used as molecular diagnostic tools in space.					
29775210	4	88	theme	proton	584:589	arg1	irradiation					591:601	low-energy proton irradiation	573:601	low-energy proton irradiation	573:601	The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation.					
29475941	9	0	from	enzymes	1290:1296	arg1	cells					1361:1365	the triple KO cells	1347:1365	the triple KO cells	1347:1365	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	1	theme	acid	1331:1334	arg1	lipase					1336:1341	lysosomal acid lipase	1321:1341	lysosomal acid lipase	1321:1341	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	1	theme	acid	1331:1334	arg1	enzymes					1290:1296	two lysosomal enzymes	1276:1296	two lysosomal enzymes	1276:1296	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	2	theme	KO	1358:1359	arg1	cells					1361:1365	the triple KO cells	1347:1365	the triple KO cells	1347:1365	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	1	3	theme	pharmaceutical	201:214	arg1	agents					216:221	very important pharmaceutical agents	186:221	very important pharmaceutical agents	186:221	Recombinant therapeutic proteins are becoming very important pharmaceutical agents for treating intractable diseases.					
29475941	9	4	theme	endoglycosidase	1429:1443	arg1	treatment					1447:1455	endoglycosidase H treatment	1429:1455	endoglycosidase H treatment	1429:1455	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	10	5	theme	complex	1568:1574	arg1	types					1576:1580	complex types	1568:1580	complex types	1568:1580	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	4	6	dep	N-linked	616:623	arg1	low-complexity					626:639	low-complexity	626:639	low-complexity	626:639	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	3	7	gly	heterogeneity	493:505	arg1	glycans					510:516	glycans	510:516	glycans attached to the produced protein	510:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	6	8	theme	complex-type	897:908	arg1	glycans					910:916	complex-type glycans	897:916	complex-type glycans	897:916	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	6	9	theme	increased	855:863	arg1	high-mannose-type					865:881	increased high-mannose-type	855:881	increased high-mannose-type	855:881	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	7	10	theme	remaining	950:958	arg1	glycans					973:979	the remaining complex-type glycans	946:979	the remaining complex-type glycans	946:979	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	9	11	theme	H	1445:1445	arg1	treatment					1447:1455	endoglycosidase H treatment	1429:1455	endoglycosidase H treatment	1429:1455	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	10	12	theme	protein	1611:1617	arg1	level					1619:1623	the protein level	1607:1623	the protein level	1607:1623	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	11	13	theme	HEK293	1666:1671	arg1	cells					1673:1677	the triple KO HEK293 cells	1652:1677	the triple KO HEK293 cells	1652:1677	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	11	13	theme	HEK293	1666:1671	arg1	suitable					1683:1690	suitable	1683:1690	suitable	1683:1690	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	10	14	theme	N-glycan	1462:1469	arg1	structures					1471:1480	The N-glycan structures	1458:1480	The N-glycan structures on recombinant proteins expressed in triple KO cells	1458:1533	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	5	15	theme	Golgi	690:694	arg1	MAN1A2					721:726	MAN1A2	721:726	MAN1A2	721:726	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	5	15	theme	Golgi	690:694	arg1	MAN1A1					710:715	MAN1A1	710:715	MAN1A1	710:715	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	5	15	theme	Golgi	690:694	arg1	mannosidases					696:707	Golgi mannosidases	690:707	Golgi mannosidases (MAN1A1 and MAN1A2)	690:727	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	8	16	dep	KO	1149:1150	arg1	MAN1A2					1161:1166	MAN1A2	1161:1166	MAN1A2	1161:1166	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	16	dep	KO	1149:1150	arg1	MAN1B1					1173:1178	MAN1B1	1173:1178	MAN1B1	1173:1178	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	16	dep	KO	1149:1150	arg1	MAN1A1					1153:1158	MAN1A1	1153:1158	MAN1A1	1153:1158	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	7	17	theme	complex-type	960:971	arg1	glycans					973:979	the remaining complex-type glycans	946:979	the remaining complex-type glycans	946:979	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	0	18	theme	high-mannose-type	111:127	arg1	N-glycans					129:137	high-mannose-type N-glycans	111:137	high-mannose-type N-glycans	111:137	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	9	19	from	glycans	1386:1392	arg1	enzymes					1403:1409	these enzymes	1397:1409	these enzymes	1397:1409	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	7	20	theme	reticulum	1041:1049	arg1	MAN1B1					1066:1071	MAN1B1	1066:1071	MAN1B1	1066:1071	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	7	20	theme	reticulum	1041:1049	arg1	I					1063:1063	the endoplasmic reticulum mannosidase I	1025:1063	the endoplasmic reticulum mannosidase I (MAN1B1)	1025:1072	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	11	21	with	enzymes	1748:1754	arg1	N-glycans					1779:1787	high-mannose-type N-glycans	1761:1787	high-mannose-type N-glycans	1761:1787	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	3	22	theme	protein	422:428	arg1	production					430:439	protein production	422:439	protein production in mammalian cells	422:458	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	11	23	theme	recombinant	1706:1716	arg1	proteins					1718:1725	recombinant proteins	1706:1725	recombinant proteins	1706:1725	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	11	23	theme	recombinant	1706:1716	arg1	enzymes					1748:1754	lysosomal enzymes	1738:1754	lysosomal enzymes with high-mannose-type N-glycans	1738:1787	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	7	24	from	knocking	996:1003	arg1	cells					1091:1095	the double KO cells	1077:1095	the double KO cells	1077:1095	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	7	25	theme	endoplasmic	1029:1039	arg1	reticulum					1041:1049	the endoplasmic reticulum	1025:1049	the endoplasmic reticulum mannosidase I (MAN1B1)	1025:1072	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	1	26	theme	intractable	236:246	arg1	diseases					248:255	intractable diseases	236:255	intractable diseases	236:255	Recombinant therapeutic proteins are becoming very important pharmaceutical agents for treating intractable diseases.					
29475941	4	27	link	N-linked	616:623	arg1	glycans					641:647	high-mannose-type N-linked, low-complexity glycans	598:647	high-mannose-type N-linked, low-complexity glycans	598:647	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	3	28	contain	has	460:462	arg1	production					430:439	protein production	422:439	protein production in mammalian cells	422:458	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	3	28	contain	has	460:462	arg2	heterogeneity					493:505	heterogeneity	493:505	heterogeneity of glycans attached to the produced protein	493:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	3	28	contain	has	460:462	arg2	drawbacks					472:480	several drawbacks	464:480	several drawbacks	464:480	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	2	29	theme	correct	343:349	arg1	folding					351:357	folding	351:357	folding	351:357	Most biopharmaceutical proteins are produced in mammalian cells because this ensures correct folding and glycosylation for protein stability and function.					
29475941	3	30	theme	several	464:470	arg1	drawbacks					472:480	several drawbacks	464:480	several drawbacks	464:480	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	3	30	theme	several	464:470	arg1	heterogeneity					493:505	heterogeneity	493:505	heterogeneity of glycans attached to the produced protein	493:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	8	31	from	structures	1240:1249	arg1	cells					1181:1185	triple KO (MAN1A1, MAN1A2, and MAN1B1) cells	1142:1185	triple KO (MAN1A1, MAN1A2, and MAN1B1) cells	1142:1185	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	10	32	theme	high-mannose	1585:1596	arg1	types					1598:1602	high-mannose types	1585:1602	high-mannose types at the protein level	1585:1623	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	3	33	theme	glycans	510:516	arg1	heterogeneity					493:505	heterogeneity	493:505	heterogeneity of glycans attached to the produced protein	493:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	0	34	theme	Genetic	0:6	arg1	disruption					8:17	Genetic disruption	0:17	Genetic disruption of multiple α1,2-mannosidases	0:47	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	10	35	theme	recombinant	1485:1495	arg1	proteins					1497:1504	recombinant proteins	1485:1504	recombinant proteins expressed in triple KO cells	1485:1533	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	3	36	attach	attached	518:525	arg2	glycans					510:516	glycans	510:516	glycans attached to the produced protein	510:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	3	36	attach	attached	518:525	arg1	protein					543:549	the produced protein	530:549	the produced protein	530:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	11	37	theme	KO	1663:1664	arg1	cells					1673:1677	the triple KO HEK293 cells	1652:1677	the triple KO HEK293 cells	1652:1677	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	11	37	theme	KO	1663:1664	arg1	suitable					1683:1690	suitable	1683:1690	suitable	1683:1690	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	5	38	theme	HEK293	732:737	arg1	cells					739:743	HEK293 cells	732:743	HEK293 cells	732:743	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	0	39	theme	multiple	22:29	arg1	α1,2-mannosidases					31:47	multiple α1,2-mannosidases	22:47	multiple α1,2-mannosidases	22:47	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	10	40	theme	triple	1519:1524	arg1	cells					1529:1533	triple KO cells	1519:1533	triple KO cells	1519:1533	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	8	41	theme	triple	1142:1147	arg1	KO					1149:1150	triple KO	1142:1150	triple KO (MAN1A1, MAN1A2, and MAN1B1) cells	1142:1185	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	6	42	theme	KO	836:837	arg1	cells					839:843	double KO cells	829:843	double KO cells	829:843	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	2	43	theme	protein	381:387	arg1	stability					389:397	protein stability	381:397	protein stability	381:397	Most biopharmaceutical proteins are produced in mammalian cells because this ensures correct folding and glycosylation for protein stability and function.					
29475941	6	44	dep	knockout	753:760	arg1	KO					763:764	KO	763:764	KO	763:764	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	8	45	theme	major	1225:1229	arg1	Man9GlcNAc2					1188:1198	Man9GlcNAc2	1188:1198	Man9GlcNAc2	1188:1198	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	45	theme	major	1225:1229	arg1	structures					1240:1249	the major N-glycan structures	1221:1249	the major N-glycan structures	1221:1249	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	45	theme	major	1225:1229	arg1	Man8GlcNAc2					1204:1214	Man8GlcNAc2	1204:1214	Man8GlcNAc2	1204:1214	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	1	46	theme	Recombinant	140:150	arg1	proteins					164:171	Recombinant therapeutic proteins	140:171	Recombinant therapeutic proteins	140:171	Recombinant therapeutic proteins are becoming very important pharmaceutical agents for treating intractable diseases.					
29475941	6	47	theme	knockout	753:760	arg1	cells					767:771	Single knockout (KO) cells	746:771	Single knockout (KO) cells	746:771	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	10	48	from	structures	1471:1480	arg1	proteins					1497:1504	recombinant proteins	1485:1504	recombinant proteins expressed in triple KO cells	1485:1533	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	10	49	theme	KO	1526:1527	arg1	cells					1529:1533	triple KO cells	1519:1533	triple KO cells	1519:1533	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	3	50	from	production	430:439	arg1	cells					454:458	mammalian cells	444:458	mammalian cells	444:458	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	2	51	theme	mammalian	306:314	arg1	cells					316:320	mammalian cells	306:320	mammalian cells	306:320	Most biopharmaceutical proteins are produced in mammalian cells because this ensures correct folding and glycosylation for protein stability and function.					
29475941	6	52	theme	Single	746:751	arg1	cells					767:771	Single knockout (KO) cells	746:771	Single knockout (KO) cells	746:771	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	3	53	theme	produced	534:541	arg1	protein					543:549	the produced protein	530:549	the produced protein	530:549	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
29475941	0	54	theme	α1,2-mannosidases	31:47	arg1	disruption					8:17	Genetic disruption	0:17	Genetic disruption of multiple α1,2-mannosidases	0:47	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	4	55	theme	N-linked	616:623	arg1	glycans					641:647	high-mannose-type N-linked, low-complexity glycans	598:647	high-mannose-type N-linked, low-complexity glycans	598:647	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	7	56	theme	complex-type	1117:1128	arg1	glycans					1130:1136	the complex-type glycans	1113:1136	the complex-type glycans	1113:1136	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	8	57	theme	N-glycan	1231:1238	arg1	Man9GlcNAc2					1188:1198	Man9GlcNAc2	1188:1198	Man9GlcNAc2	1188:1198	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	57	theme	N-glycan	1231:1238	arg1	structures					1240:1249	the major N-glycan structures	1221:1249	the major N-glycan structures	1221:1249	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	8	57	theme	N-glycan	1231:1238	arg1	Man8GlcNAc2					1204:1214	Man8GlcNAc2	1204:1214	Man8GlcNAc2	1204:1214	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	6	58	theme	double	829:834	arg1	cells					839:843	double KO cells	829:843	double KO cells	829:843	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	10	59	from	level	1619:1623	arg1	types					1598:1602	high-mannose types	1585:1602	high-mannose types at the protein level	1585:1623	The N-glycan structures on recombinant proteins expressed in triple KO cells were simplified and changed from complex types to high-mannose types at the protein level.					
29475941	4	60	theme	high-mannose-type	598:614	arg1	glycans					641:647	high-mannose-type N-linked, low-complexity glycans	598:647	high-mannose-type N-linked, low-complexity glycans	598:647	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	9	61	theme	lysosomal	1280:1288	arg1	lipase					1336:1341	lysosomal acid lipase	1321:1341	lysosomal acid lipase	1321:1341	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	61	theme	lysosomal	1280:1288	arg1	α-galactosidase-A					1299:1315	α-galactosidase-A	1299:1315	α-galactosidase-A	1299:1315	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	61	theme	lysosomal	1280:1288	arg1	enzymes					1290:1296	two lysosomal enzymes	1276:1296	two lysosomal enzymes	1276:1296	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	7	62	theme	KO	1088:1089	arg1	cells					1091:1095	the double KO cells	1077:1095	the double KO cells	1077:1095	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	1	63	theme	therapeutic	152:162	arg1	proteins					164:171	Recombinant therapeutic proteins	140:171	Recombinant therapeutic proteins	140:171	Recombinant therapeutic proteins are becoming very important pharmaceutical agents for treating intractable diseases.					
29475941	0	64	theme	mammalian	59:67	arg1	cells					69:73	mammalian cells	59:73	mammalian cells producing recombinant proteins with high-mannose-type N-glycans	59:137	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	9	65	theme	lysosomal	1321:1329	arg1	lipase					1336:1341	lysosomal acid lipase	1321:1341	lysosomal acid lipase	1321:1341	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	9	65	theme	lysosomal	1321:1329	arg1	enzymes					1290:1296	two lysosomal enzymes	1276:1296	two lysosomal enzymes	1276:1296	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	11	66	theme	high-mannose-type	1761:1777	arg1	N-glycans					1779:1787	high-mannose-type N-glycans	1761:1787	high-mannose-type N-glycans	1761:1787	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	4	67	with	lines	587:591	arg1	glycans					641:647	high-mannose-type N-linked, low-complexity glycans	598:647	high-mannose-type N-linked, low-complexity glycans	598:647	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	5	68	dep	mannosidases	696:707	arg1	MAN1A2					721:726	MAN1A2	721:726	MAN1A2	721:726	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	5	68	dep	mannosidases	696:707	arg1	MAN1A1					710:715	MAN1A1	710:715	MAN1A1	710:715	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	5	68	dep	mannosidases	696:707	arg1	mannosidases					696:707	Golgi mannosidases	690:707	Golgi mannosidases (MAN1A1 and MAN1A2)	690:727	We first knocked out two genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) in HEK293 cells.					
29475941	9	69	theme	triple	1351:1356	arg1	cells					1361:1365	the triple KO cells	1347:1365	the triple KO cells	1347:1365	Therefore, we expressed two lysosomal enzymes, α-galactosidase-A and lysosomal acid lipase, in the triple KO cells and found that the glycans on these enzymes were sensitive to endoglycosidase H treatment.					
29475941	11	70	theme	lysosomal	1738:1746	arg1	enzymes					1748:1754	lysosomal enzymes	1738:1754	lysosomal enzymes with high-mannose-type N-glycans	1738:1787	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	2	71	theme	biopharmaceutical	263:279	arg1	proteins					281:288	Most biopharmaceutical proteins	258:288	Most biopharmaceutical proteins	258:288	Most biopharmaceutical proteins are produced in mammalian cells because this ensures correct folding and glycosylation for protein stability and function.					
29475941	7	72	theme	mannosidase	1051:1061	arg1	MAN1B1					1066:1071	MAN1B1	1066:1071	MAN1B1	1066:1071	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	7	72	theme	mannosidase	1051:1061	arg1	I					1063:1063	the endoplasmic reticulum mannosidase I	1025:1063	the endoplasmic reticulum mannosidase I (MAN1B1)	1025:1072	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	2	73	theme	Most	258:261	arg1	proteins					281:288	Most biopharmaceutical proteins	258:288	Most biopharmaceutical proteins	258:288	Most biopharmaceutical proteins are produced in mammalian cells because this ensures correct folding and glycosylation for protein stability and function.					
29475941	7	74	theme	double	1081:1086	arg1	cells					1091:1095	the double KO cells	1077:1095	the double KO cells	1077:1095	In our effort to eliminate the remaining complex-type glycans, we found that knocking out a gene encoding the endoplasmic reticulum mannosidase I (MAN1B1) in the double KO cells reduced most of the complex-type glycans.					
29475941	6	75	theme	N-glycan	800:807	arg1	structures					809:818	N-glycan structures	800:818	N-glycan structures	800:818	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	0	76	theme	recombinant	85:95	arg1	proteins					97:104	recombinant proteins	85:104	recombinant proteins	85:104	Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.					
29475941	11	77	theme	triple	1656:1661	arg1	cells					1673:1677	the triple KO HEK293 cells	1652:1677	the triple KO HEK293 cells	1652:1677	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	11	77	theme	triple	1656:1661	arg1	suitable					1683:1690	suitable	1683:1690	suitable	1683:1690	Our results indicate that the triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans.					
29475941	6	78	from	changes	789:795	arg1	structures					809:818	N-glycan structures	800:818	N-glycan structures	800:818	Single knockout (KO) cells did not exhibit changes in N-glycan structures, whereas double KO cells displayed increased high-mannose-type and decreased complex-type glycans.					
29475941	4	79	theme	cell	582:585	arg1	lines					587:591	cell lines	582:591	cell lines with high-mannose-type N-linked, low-complexity glycans	582:647	In this study, we established cell lines with high-mannose-type N-linked, low-complexity glycans.					
29475941	1	80	theme	important	191:199	arg1	agents					216:221	very important pharmaceutical agents	186:221	very important pharmaceutical agents	186:221	Recombinant therapeutic proteins are becoming very important pharmaceutical agents for treating intractable diseases.					
29475941	8	81	theme	KO	1149:1150	arg1	cells					1181:1185	triple KO (MAN1A1, MAN1A2, and MAN1B1) cells	1142:1185	triple KO (MAN1A1, MAN1A2, and MAN1B1) cells	1142:1185	In triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 were the major N-glycan structures.					
29475941	3	82	theme	mammalian	444:452	arg1	cells					454:458	mammalian cells	444:458	mammalian cells	444:458	However, protein production in mammalian cells has several drawbacks, including heterogeneity of glycans attached to the produced protein.					
30037551	6	0	dep	effective	1016:1024	arg1	evidenced					1114:1122	evidenced	1114:1122	evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation	1114:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	0	1	theme	role	193:196	arg1	determination					198:210	biological role determination	182:210	biological role determination of glycoconjugates	182:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	6	2	theme	Immobilized	986:996	arg1	glycosidases					998:1009	Immobilized glycosidases	986:1009	Immobilized glycosidases	986:1009	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	4	3	theme	oryzae	745:750	arg1	β-galactosidase					752:766	Aspergillus oryzae β-galactosidase	733:766	Aspergillus oryzae β-galactosidase	733:766	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	11	4	theme	glycans	1824:1830	arg1	functions					1843:1851	glycans biological functions	1824:1851	glycans biological functions	1824:1851	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	8	5	theme	native	1379:1384	arg1	glycoconjugates					1386:1400	native glycoconjugates	1379:1400	native glycoconjugates	1379:1400	Their use was extended to the elucidation of the glycan role of native glycoconjugates.					
30037551	6	6	gly	glycoproteins	1068:1080	arg1	glycoproteins					1068:1080	model glycoproteins	1062:1080	model glycoproteins	1062:1080	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	2	7	theme	biological	360:369	arg1	function					371:378	biological function	360:378	biological function	360:378	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	5	8	theme	original	967:974	arg1	activity					976:983	its original activity	963:983	its original activity	963:983	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	4	9	theme	individual	704:713	arg1	immobilization					715:728	the individual immobilization	700:728	the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups	700:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	0	10	theme	valuable	127:134	arg1	tools					145:149	valuable glycomic tools	127:149	valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates	127:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	3	11	theme	specific	620:627	arg1	carbohydrates					629:641	specific carbohydrates	620:641	specific carbohydrates	620:641	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	6	12	theme	glycoproteins	1068:1080	arg1	hepatica					1097:1104	a Fasciola hepatica	1086:1104	a Fasciola hepatica	1086:1104	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	6	12	theme	glycoproteins	1068:1080	arg1	deglycosylation					1043:1057	the selective deglycosylation	1029:1057	the selective deglycosylation of model glycoproteins	1029:1080	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	0	13	theme	tools	145:149	arg1	potentiality					111:122	the potentiality	107:122	the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates	107:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	1	14	contain	have	278:281	arg2	roles					317:321	several structural and functional roles	283:321	several structural and functional roles	283:321	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	1	14	contain	have	278:281	arg1	Glycans					232:238	Glycans	232:238	Glycans present in biological glycoconjugates	232:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	1	15	from	present	240:246	arg1	glycoconjugates					262:276	biological glycoconjugates	251:276	biological glycoconjugates	251:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	7	16	theme	several	1253:1259	arg1	cycles					1277:1282	several deglycosylation cycles	1253:1282	several deglycosylation cycles without loss of effectiveness	1253:1312	Immobilized glycosidases were reused for several deglycosylation cycles without loss of effectiveness.					
30037551	0	17	theme	glycosylation	155:167	arg1	analysis					169:176	glycosylation analysis	155:176	glycosylation analysis	155:176	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	6	18	theme	selective	1033:1041	arg1	deglycosylation					1043:1057	the selective deglycosylation	1029:1057	the selective deglycosylation of model glycoproteins	1029:1080	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	4	19	theme	magnetic	831:838	arg1	nanoparticles					840:852	agarose and silica magnetic nanoparticles	812:852	agarose and silica magnetic nanoparticles activated with cyanate ester groups	812:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	1	20	from	glycoconjugates	262:276	arg1	present					240:246	present	240:246	present	240:246	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	4	21	theme	ensiformis	782:791	arg1	α-mannosidase					793:805	Canavalia ensiformis α-mannosidase	772:805	Canavalia ensiformis α-mannosidase	772:805	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	0	22	theme	β-galactosidase	18:32	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.	0:230	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	5	23	theme	High	891:894	arg1	yields					911:916	High immobilization yields	891:916	High immobilization yields (70-90%)	891:925	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	5	23	theme	High	891:894	arg1	%					924:924	70-90%	919:924	70-90%	919:924	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	10	24	theme	hepatica	1565:1572	arg1	lysate					1574:1579	a F. hepatica lysate	1560:1579	a F. hepatica lysate	1560:1579	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	9	25	theme	lactoferrin	1436:1446	arg1	recognition					1421:1431	the recognition	1417:1431	the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN	1417:1510	A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found.					
30037551	6	26	theme	hepatica	1097:1104	arg1	lysate					1106:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	6	27	from	lysate	1106:1111	arg1	effective					1016:1024	effective	1016:1024	effective	1016:1024	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	2	28	theme	disease	509:515	arg1	treatment					517:525	disease treatment	509:525	disease treatment	509:525	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	2	29	theme	pathogenic	438:447	arg1	process					449:455	physiological and pathogenic process	420:455	physiological and pathogenic process	420:455	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	2	30	theme	methods	497:503	arg1	development					471:481	the development	467:481	the development of diagnostic methods	467:503	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	2	30	theme	methods	497:503	arg1	treatment					517:525	disease treatment	509:525	disease treatment	509:525	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	4	31	theme	cyanate	869:875	arg1	groups					883:888	cyanate ester groups	869:888	cyanate ester groups	869:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	3	32	theme	Immobilized	528:538	arg1	tools					566:570	powerful tools	557:570	powerful tools for glycan analysis	557:590	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	3	32	theme	Immobilized	528:538	arg1	glycosidases					540:551	Immobilized glycosidases	528:551	Immobilized glycosidases	528:551	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	7	33	theme	effectiveness	1300:1312	arg1	loss					1292:1295	loss	1292:1295	loss of effectiveness	1292:1312	Immobilized glycosidases were reused for several deglycosylation cycles without loss of effectiveness.					
30037551	9	34	theme	C-type	1480:1485	arg1	DC-SIGN					1504:1510	a C-type lectin receptor, DC-SIGN	1478:1510	DC-SIGN	1504:1510	A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found.					
30037551	0	35	gly	glycosylation	155:167	arg1	glycoconjugates					215:229	glycoconjugates	215:229	glycoconjugates	215:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	8	36	theme	role	1371:1374	arg1	elucidation					1345:1355	the elucidation	1341:1355	the elucidation of the glycan role of native glycoconjugates	1341:1400	Their use was extended to the elucidation of the glycan role of native glycoconjugates.					
30037551	9	37	theme	receptor	1494:1501	arg1	DC-SIGN					1504:1510	a C-type lectin receptor, DC-SIGN	1478:1510	DC-SIGN	1504:1510	A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found.					
30037551	0	38	theme	α-mannosidase	38:50	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.	0:230	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	6	39	theme	specific	1141:1148	arg1	recognition					1157:1167	specific lectin recognition	1141:1167	specific lectin recognition of 40-50% after enzymatic deglycosylation	1141:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	10	40	theme	F.	1562:1563	arg1	lysate					1574:1579	a F. hepatica lysate	1560:1579	a F. hepatica lysate	1560:1579	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	10	41	theme	responses	1668:1676	arg1	induction					1639:1647	the induction	1635:1647	the induction of Th2/Treg immune responses during the infection	1635:1697	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	11	42	theme	glycosidases	1755:1766	arg1	immobilization					1728:1741	successful immobilization	1717:1741	successful immobilization of specific glycosidases in nano-supports	1717:1783	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	0	43	theme	magnetic	57:64	arg1	nanoparticles					66:78	magnetic nanoparticles	57:78	magnetic nanoparticles	57:78	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	10	44	theme	Th2/Treg	1652:1659	arg1	responses					1668:1676	Th2/Treg immune responses	1652:1676	Th2/Treg immune responses	1652:1676	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	1	45	theme	biological	251:260	arg1	glycoconjugates					262:276	biological glycoconjugates	251:276	biological glycoconjugates	251:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	6	46	from	effective	1016:1024	arg1	lysate					1106:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	1	47	attach	present	240:246	arg2	Glycans					232:238	Glycans	232:238	Glycans present in biological glycoconjugates	232:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	1	47	attach	present	240:246	arg1	glycoconjugates					262:276	biological glycoconjugates	251:276	biological glycoconjugates	251:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	2	48	theme	physiological	420:432	arg1	process					449:455	physiological and pathogenic process	420:455	physiological and pathogenic process	420:455	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	8	49	theme	glycan	1364:1369	arg1	role					1371:1374	the glycan role	1360:1374	the glycan role of native glycoconjugates	1360:1400	Their use was extended to the elucidation of the glycan role of native glycoconjugates.					
30037551	9	50	from	decrease	1405:1412	arg1	recognition					1421:1431	the recognition	1417:1431	the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN	1417:1510	A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found.					
30037551	0	51	dep	Immobilization	0:13	arg1	strategy					83:90	A strategy	81:90	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.	0:230	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	3	52	theme	glycan	576:581	arg1	analysis					583:590	glycan analysis	576:590	glycan analysis	576:590	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	1	53	theme	structural	291:300	arg1	roles					317:321	several structural and functional roles	283:321	several structural and functional roles	283:321	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	0	54	theme	glycoconjugates	215:229	arg1	analysis					169:176	glycosylation analysis	155:176	glycosylation analysis	155:176	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	0	54	theme	glycoconjugates	215:229	arg1	determination					198:210	biological role determination	182:210	biological role determination of glycoconjugates	182:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	2	55	theme	glycan	339:344	arg1	structure					346:354	glycan structure	339:354	glycan structure	339:354	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	1	56	theme	functional	306:315	arg1	roles					317:321	several structural and functional roles	283:321	several structural and functional roles	283:321	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	7	57	theme	Immobilized	1212:1222	arg1	glycosidases					1224:1235	Immobilized glycosidases	1212:1235	Immobilized glycosidases	1212:1235	Immobilized glycosidases were reused for several deglycosylation cycles without loss of effectiveness.					
30037551	6	58	gly	deglycosylation	1043:1057	arg1	glycoproteins					1068:1080	model glycoproteins	1062:1080	model glycoproteins	1062:1080	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	2	59	from	role	412:415	arg1	process					449:455	physiological and pathogenic process	420:455	physiological and pathogenic process	420:455	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	4	60	theme	Aspergillus	733:743	arg1	oryzae					745:750	Aspergillus oryzae	733:750	Aspergillus oryzae β-galactosidase	733:766	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	11	61	theme	biological	1832:1841	arg1	functions					1843:1851	glycans biological functions	1824:1851	glycans biological functions	1824:1851	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	11	62	theme	successful	1717:1726	arg1	immobilization					1728:1741	successful immobilization	1717:1741	successful immobilization of specific glycosidases in nano-supports	1717:1783	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	2	63	theme	function	371:378	arg1	Elucidation					324:334	Elucidation	324:334	Elucidation of glycan structure and biological function	324:378	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	6	64	theme	Fasciola	1088:1095	arg1	hepatica					1097:1104	a Fasciola hepatica	1086:1104	a Fasciola hepatica	1086:1104	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	4	65	theme	β-galactosidase	752:766	arg1	immobilization					715:728	the individual immobilization	700:728	the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups	700:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	0	66	theme	glycomic	136:143	arg1	tools					145:149	valuable glycomic tools	127:149	valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates	127:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	10	67	theme	specific	1532:1539	arg1	deglycosylation					1541:1555	the specific deglycosylation	1528:1555	the specific deglycosylation of a F. hepatica lysate	1528:1579	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	11	68	from	immobilization	1728:1741	arg1	nano-supports					1771:1783	nano-supports	1771:1783	nano-supports	1771:1783	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	8	69	theme	glycoconjugates	1386:1400	arg1	role					1371:1374	the glycan role	1360:1374	the glycan role of native glycoconjugates	1360:1400	Their use was extended to the elucidation of the glycan role of native glycoconjugates.					
30037551	3	70	theme	protein	664:670	arg1	structure					672:680	the protein structure	660:680	the protein structure	660:680	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	1	71	theme	present	240:246	arg1	Glycans					232:238	Glycans	232:238	Glycans present in biological glycoconjugates	232:276	Glycans present in biological glycoconjugates have several structural and functional roles.					
30037551	6	72	theme	model	1062:1066	arg1	glycoproteins					1068:1080	model glycoproteins	1062:1080	model glycoproteins	1062:1080	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	6	73	theme	deglycosylation	1043:1057	arg1	lysate					1106:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate	1029:1111	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	4	74	theme	agarose	812:818	arg1	nanoparticles					840:852	agarose and silica magnetic nanoparticles	812:852	agarose and silica magnetic nanoparticles activated with cyanate ester groups	812:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	0	75	theme	biological	182:191	arg1	determination					198:210	biological role determination	182:210	biological role determination of glycoconjugates	182:229	Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates.					
30037551	4	76	theme	silica	824:829	arg1	nanoparticles					840:852	agarose and silica magnetic nanoparticles	812:852	agarose and silica magnetic nanoparticles activated with cyanate ester groups	812:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	5	77	theme	activity	976:983	arg1	activity					976:983	its original activity	963:983	its original activity	963:983	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	5	77	theme	activity	976:983	arg1	%					958:958	60%	956:958	60% of its original activity	956:983	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	4	78	theme	Canavalia	772:780	arg1	α-mannosidase					793:805	Canavalia ensiformis α-mannosidase	772:805	Canavalia ensiformis α-mannosidase	772:805	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	10	79	gly	deglycosylation	1541:1555	arg1	lysate					1574:1579	a F. hepatica lysate	1560:1579	a F. hepatica lysate	1560:1579	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	10	80	theme	lysate	1574:1579	arg1	deglycosylation					1541:1555	the specific deglycosylation	1528:1555	the specific deglycosylation of a F. hepatica lysate	1528:1579	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	4	81	theme	α-mannosidase	793:805	arg1	immobilization					715:728	the individual immobilization	700:728	the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups	700:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	2	82	theme	structure	346:354	arg1	Elucidation					324:334	Elucidation	324:334	Elucidation of glycan structure and biological function	324:378	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	6	83	from	decrease	1129:1136	arg1	recognition					1157:1167	specific lectin recognition	1141:1167	specific lectin recognition of 40-50% after enzymatic deglycosylation	1141:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	4	84	theme	ester	877:881	arg1	groups					883:888	cyanate ester groups	869:888	cyanate ester groups	869:888	Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups.					
30037551	6	85	theme	enzymatic	1185:1193	arg1	deglycosylation					1195:1209	enzymatic deglycosylation	1185:1209	enzymatic deglycosylation	1185:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	6	86	theme	%	1177:1177	arg1	recognition					1157:1167	specific lectin recognition	1141:1167	specific lectin recognition of 40-50% after enzymatic deglycosylation	1141:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	10	87	theme	mannosylated	1611:1622	arg1	glycans					1624:1630	mannosylated glycans	1611:1630	mannosylated glycans	1611:1630	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	2	88	theme	diagnostic	486:495	arg1	methods					497:503	diagnostic methods	486:503	diagnostic methods	486:503	Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment.					
30037551	5	89	theme	immobilization	896:909	arg1	yields					911:916	High immobilization yields	891:916	High immobilization yields (70-90%)	891:925	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	5	89	theme	immobilization	896:909	arg1	%					924:924	70-90%	919:924	70-90%	919:924	High immobilization yields (70-90%) were achieved, keeping above 60% of its original activity.					
30037551	9	90	theme	lectin	1487:1492	arg1	DC-SIGN					1504:1510	a C-type lectin receptor, DC-SIGN	1478:1510	DC-SIGN	1504:1510	A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found.					
30037551	6	91	theme	lectin	1150:1155	arg1	recognition					1157:1167	specific lectin recognition	1141:1167	specific lectin recognition of 40-50% after enzymatic deglycosylation	1141:1209	Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40-50% after enzymatic deglycosylation.					
30037551	10	92	theme	glycans	1624:1630	arg1	relevance					1598:1606	the relevance	1594:1606	the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection	1594:1697	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	11	93	theme	specific	1746:1753	arg1	glycosidases					1755:1766	specific glycosidases	1746:1766	specific glycosidases	1746:1766	Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.					
30037551	7	94	theme	deglycosylation	1261:1275	arg1	cycles					1277:1282	several deglycosylation cycles	1253:1282	several deglycosylation cycles without loss of effectiveness	1253:1312	Immobilized glycosidases were reused for several deglycosylation cycles without loss of effectiveness.					
30037551	10	95	from	relevance	1598:1606	arg1	induction					1639:1647	the induction	1635:1647	the induction of Th2/Treg immune responses during the infection	1635:1697	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	10	96	theme	immune	1661:1666	arg1	responses					1668:1676	Th2/Treg immune responses	1652:1676	Th2/Treg immune responses	1652:1676	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	10	97	gly	mannosylated	1611:1622	arg1	glycans					1624:1630	mannosylated glycans	1611:1630	mannosylated glycans	1611:1630	Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection.					
30037551	3	98	theme	powerful	557:564	arg1	tools					566:570	powerful tools	557:570	powerful tools for glycan analysis	557:590	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	3	98	theme	powerful	557:564	arg1	glycosidases					540:551	Immobilized glycosidases	528:551	Immobilized glycosidases	528:551	Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure.					
30037551	1	99	theme	several	283:289	arg1	roles					317:321	several structural and functional roles	283:321	several structural and functional roles	283:321	Glycans present in biological glycoconjugates have several structural and functional roles.					
30908021	7	0	theme	N-glycans	1697:1705	arg1	diversity					1675:1683	the tremendous structural diversity	1649:1683	the tremendous structural diversity of sulfated N-glycans	1649:1705	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	6	1	theme	vaccines	1434:1441	arg1	sulfo-N-glycans					1486:1500	those highly branched nonsialyl sulfo-N-glycans	1454:1500	those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1454:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	1	theme	vaccines	1434:1441	arg1	sets					1416:1419	large sets	1410:1419	large sets	1410:1419	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	1	theme	vaccines	1434:1441	arg1	vaccines					1434:1441	influenza vaccines	1424:1441	influenza vaccines	1424:1441	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	3	2	theme	viral	596:600	arg1	hemagglutinin					602:614	influenza viral hemagglutinin	586:614	influenza viral hemagglutinin	586:614	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	6	3	theme	database-aided	1305:1318	arg1	analyses					1335:1342	The database-aided glycoproteomic analyses	1301:1342	The database-aided glycoproteomic analyses	1301:1342	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	3	4	theme	influenza	586:594	arg1	hemagglutinin					602:614	influenza viral hemagglutinin	586:614	influenza viral hemagglutinin	586:614	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	7	5	theme	structural	1664:1673	arg1	diversity					1675:1683	the tremendous structural diversity	1649:1683	the tremendous structural diversity of sulfated N-glycans	1649:1705	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	4	6	theme	glycopeptide	945:956	arg1	sequencing					958:967	de novo glycopeptide sequencing	937:967	de novo glycopeptide sequencing	937:967	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	6	7	theme	large	1410:1414	arg1	sulfo-N-glycans					1486:1500	those highly branched nonsialyl sulfo-N-glycans	1454:1500	those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1454:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	7	theme	large	1410:1414	arg1	sets					1416:1419	large sets	1410:1419	large sets	1410:1419	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	7	theme	large	1410:1414	arg1	vaccines					1434:1441	influenza vaccines	1424:1441	influenza vaccines	1424:1441	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	1	8	theme	therapeutic	221:231	arg1	biologics					233:241	many therapeutic biologics	216:241	many therapeutic biologics	216:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	6	9	theme	structures	1396:1405	arg1	determination					1357:1369	rapid determination	1351:1369	rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1351:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	3	10	theme	influenza	659:667	arg1	vaccines					669:676	influenza vaccines	659:676	influenza vaccines	659:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	4	11	gly	glycopeptide	945:956	arg2	glycopeptide					945:956	de novo glycopeptide sequencing	937:967	de novo glycopeptide sequencing	937:967	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	6	12	theme	sulfated-N-glycan	1378:1394	arg1	structures					1396:1405	new sulfated-N-glycan structures	1374:1405	new sulfated-N-glycan structures	1374:1405	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	13	theme	nonsialyl	1476:1484	arg1	sulfo-N-glycans					1486:1500	those highly branched nonsialyl sulfo-N-glycans	1454:1500	those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1454:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	3	14	theme	existing	696:703	arg1	resource					705:712	the existing resource	692:712	the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions	692:822	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	3	15	used	utilized	683:690	arg2	we					552:553	we	552:553	we	552:553	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	5	16	theme	complex	1140:1146	arg1	structures					1126:1135	the isobaric structures	1113:1135	the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1113:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	2	17	theme	sulfated	276:283	arg1	N-glycomes					285:294	sulfated N-glycomes	276:294	sulfated N-glycomes	276:294	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	4	18	theme	site-specific	1004:1016	arg1	locations					1018:1026	the site-specific locations	1000:1026	the site-specific locations	1000:1026	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	6	19	theme	rapid	1351:1355	arg1	determination					1357:1369	rapid determination	1351:1369	rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1351:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	20	from	determination	1357:1369	arg1	sulfo-N-glycans					1486:1500	those highly branched nonsialyl sulfo-N-glycans	1454:1500	those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1454:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	20	from	determination	1357:1369	arg1	sets					1416:1419	large sets	1410:1419	large sets	1410:1419	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	20	from	determination	1357:1369	arg1	vaccines					1434:1441	influenza vaccines	1424:1441	influenza vaccines	1424:1441	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	1	21	gly	glycoproteins	199:211	arg1	glycoproteins					199:211	glycoproteins	199:211	glycoproteins in many therapeutic biologics	199:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	5	22	theme	hybrid	1152:1157	arg1	N-glycans					1159:1167	hybrid N-glycans	1152:1167	hybrid N-glycans	1152:1167	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	3	23	theme	several	639:645	arg1	subtypes					647:654	several subtypes	639:654	several subtypes of influenza vaccines	639:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	1	24	from	biologics	233:241	arg1	modifications					182:194	enzymatically post-glycosylational modifications	147:194	enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	147:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	1	25	theme	post-glycosylational	161:180	arg1	modifications					182:194	enzymatically post-glycosylational modifications	147:194	enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	147:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	2	26	theme	daunting	306:313	arg1	challenge					325:333	a daunting technical challenge	304:333	a daunting technical challenge	304:333	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	0	27	from	Diversity	22:30	arg1	Vaccines					67:74	Influenza Vaccines	57:74	Influenza Vaccines	57:74	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	1	28	from	glycoproteins	199:211	arg1	biologics					233:241	many therapeutic biologics	216:241	many therapeutic biologics	216:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	7	29	theme	glycoproteins	1795:1807	arg1	sulfation					1772:1780	N-glycan sulfation	1763:1780	N-glycan sulfation of influenza glycoproteins	1763:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	3	30	from	subtypes	647:654	arg1	N-glycans					573:581	the N-glycans	569:581	the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines	569:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	5	31	theme	sulfation	1191:1199	arg1	sequon					1201:1206	a terminal sulfation sequon	1180:1206	a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1180:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	1	32	from	modifications	182:194	arg1	biologics					233:241	many therapeutic biologics	216:241	many therapeutic biologics	216:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	4	33	theme	unique	872:877	arg1	N-glycopeptides					879:893	1380 unique N-glycopeptides	867:893	1380 unique N-glycopeptides	867:893	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	3	34	theme	liquid	511:516	arg1	spectrometry					538:549	liquid chromatography-mass spectrometry	511:549	liquid chromatography-mass spectrometry	511:549	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	0	35	theme	Sulfation	44:52	arg1	Diversity					22:30	Remarkable Structural Diversity	0:30	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines	0:74	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	0	36	theme	Remarkable	0:9	arg1	Diversity					22:30	Remarkable Structural Diversity	0:30	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines	0:74	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	5	37	theme	sulfated-4-GalNAc	1282:1298	arg1	GalNAc-GlcNAc					1226:1238	GalNAc-GlcNAc	1226:1238	GalNAc-GlcNAc	1226:1238	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	37	theme	sulfated-4-GalNAc	1282:1298	arg1	Gal-GlcNAc					1211:1220	Gal-GlcNAc	1211:1220	Gal-GlcNAc	1211:1220	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	4	38	dep	the	830:832	arg1	aid					834:836	aid	834:836	aid	834:836	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	2	39	theme	methods	496:502	arg1	lack					460:463	a lack	458:463	a lack of highly selective enrichment methods	458:502	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	39	theme	methods	496:502	arg1	composition					380:390	negatively charged heterogeneous composition	347:390	negatively charged heterogeneous composition	347:390	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	39	theme	methods	496:502	arg1	lability					421:428	lability	421:428	lability of sulfate attachments	421:451	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	39	theme	methods	496:502	arg1	structures					409:418	large molecular structures	393:418	large molecular structures	393:418	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	5	40	from	sequon	1201:1206	arg1	GalNAc-GlcNAc					1226:1238	GalNAc-GlcNAc	1226:1238	GalNAc-GlcNAc	1226:1238	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	40	from	sequon	1201:1206	arg1	Gal-GlcNAc					1211:1220	Gal-GlcNAc	1211:1220	Gal-GlcNAc	1211:1220	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	41	theme	spectrometric	1051:1063	arg1	method					1065:1070	a mass spectrometric method	1044:1070	a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1044:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	41	theme	spectrometric	1051:1063	arg1	able					1080:1083	able	1080:1083	able	1080:1083	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	2	42	theme	selective	475:483	arg1	methods					496:502	highly selective enrichment methods	468:502	highly selective enrichment methods	468:502	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	0	43	theme	N-Glycan	35:42	arg1	Sulfation					44:52	N-Glycan Sulfation	35:52	N-Glycan Sulfation	35:52	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	7	44	theme	functional	1738:1747	arg1	importance					1749:1758	potential functional importance	1728:1758	potential functional importance of N-glycan sulfation of influenza glycoproteins	1728:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	5	45	theme	isobaric	1117:1124	arg1	structures					1126:1135	the isobaric structures	1113:1135	the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1113:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	4	46	theme	de	937:938	arg1	sequencing					958:967	de novo glycopeptide sequencing	937:967	de novo glycopeptide sequencing	937:967	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	5	47	theme	mass	1046:1049	arg1	method					1065:1070	a mass spectrometric method	1044:1070	a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1044:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	47	theme	mass	1046:1049	arg1	able					1080:1083	able	1080:1083	able	1080:1083	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	3	48	theme	N-glycan	765:772	arg1	structures					774:783	927 N-glycan structures	761:783	927 N-glycan structures	761:783	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	3	49	theme	hemagglutinin	602:614	arg1	N-glycans					573:581	the N-glycans	569:581	the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines	569:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	7	50	theme	sulfated	1688:1695	arg1	N-glycans					1697:1705	sulfated N-glycans	1688:1705	sulfated N-glycans	1688:1705	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	1	51	attach	derived	134:140	arg2	structures					123:132	biologically important structures	100:132	biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	100:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	1	51	attach	derived	134:140	arg2	N-glycans					86:94	Sulfated N-glycans	77:94	Sulfated N-glycans	77:94	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	1	51	attach	derived	134:140	arg1	modifications					182:194	enzymatically post-glycosylational modifications	147:194	enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	147:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	2	52	theme	sulfate	433:439	arg1	attachments					441:451	sulfate attachments	433:451	sulfate attachments	433:451	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	7	53	gly	glycoproteins	1795:1807	arg1	glycoproteins					1795:1807	influenza glycoproteins	1785:1807	influenza glycoproteins	1785:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	3	54	theme	sulfated	793:800	arg1	compositions					811:822	387 sulfated N-glycan compositions	789:822	387 sulfated N-glycan compositions	789:822	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	6	55	theme	influenza	1424:1432	arg1	vaccines					1434:1441	influenza vaccines	1424:1441	influenza vaccines	1424:1441	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	2	56	theme	charged	358:364	arg1	composition					380:390	negatively charged heterogeneous composition	347:390	negatively charged heterogeneous composition	347:390	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	57	theme	large	393:397	arg1	structures					409:418	large molecular structures	393:418	large molecular structures	393:418	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	7	58	theme	tremendous	1653:1662	arg1	diversity					1675:1683	the tremendous structural diversity	1649:1683	the tremendous structural diversity of sulfated N-glycans	1649:1705	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	3	59	theme	neuraminidase	620:632	arg1	N-glycans					573:581	the N-glycans	569:581	the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines	569:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	1	60	theme	many	216:219	arg1	biologics					233:241	many therapeutic biologics	216:241	many therapeutic biologics	216:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	3	61	theme	vaccines	669:676	arg1	subtypes					647:654	several subtypes	639:654	several subtypes of influenza vaccines	639:676	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	7	62	theme	novel	1624:1628	arg1	findings					1630:1637	The novel findings	1620:1637	The novel findings	1620:1637	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	6	63	theme	lactosaminic	1510:1521	arg1	extensions					1523:1532	lactosaminic extensions	1510:1532	lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1510:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	4	64	dep	de	937:938	arg1	novo					940:943	novo	940:943	novo	940:943	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	6	65	theme	new	1374:1376	arg1	structures					1396:1405	new sulfated-N-glycan structures	1374:1405	new sulfated-N-glycan structures	1374:1405	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	66	theme	branched	1467:1474	arg1	sulfo-N-glycans					1486:1500	those highly branched nonsialyl sulfo-N-glycans	1454:1500	those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases	1454:1617	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	5	67	theme	terminal	1182:1189	arg1	sequon					1201:1206	a terminal sulfation sequon	1180:1206	a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1180:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	2	68	theme	N-glycomes	285:294	arg1	analysis					264:271	The high-throughput analysis	244:271	The high-throughput analysis of sulfated N-glycomes	244:294	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	5	69	theme	N-glycans	1159:1167	arg1	structures					1126:1135	the isobaric structures	1113:1135	the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc	1113:1298	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	2	70	theme	technical	315:323	arg1	challenge					325:333	a daunting technical challenge	304:333	a daunting technical challenge	304:333	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	6	71	theme	glycoproteomic	1320:1333	arg1	analyses					1335:1342	The database-aided glycoproteomic analyses	1301:1342	The database-aided glycoproteomic analyses	1301:1342	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	72	theme	hybrid	1554:1559	arg1	N-glycans					1561:1569	hybrid N-glycans	1554:1569	hybrid N-glycans	1554:1569	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	1	73	theme	glycoproteins	199:211	arg1	modifications					182:194	enzymatically post-glycosylational modifications	147:194	enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	147:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	7	74	theme	influenza	1785:1793	arg1	glycoproteins					1795:1807	influenza glycoproteins	1785:1807	influenza glycoproteins	1785:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	2	75	theme	high-throughput	248:262	arg1	analysis					264:271	The high-throughput analysis	244:271	The high-throughput analysis of sulfated N-glycomes	244:294	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	5	76	theme	sulfated-3-Gal	1243:1256	arg1	GalNAc-GlcNAc					1226:1238	GalNAc-GlcNAc	1226:1238	GalNAc-GlcNAc	1226:1238	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	76	theme	sulfated-3-Gal	1243:1256	arg1	Gal-GlcNAc					1211:1220	Gal-GlcNAc	1211:1220	Gal-GlcNAc	1211:1220	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	2	77	theme	heterogeneous	366:378	arg1	composition					380:390	negatively charged heterogeneous composition	347:390	negatively charged heterogeneous composition	347:390	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	7	78	theme	sulfation	1772:1780	arg1	importance					1749:1758	potential functional importance	1728:1758	potential functional importance of N-glycan sulfation of influenza glycoproteins	1728:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	6	79	from	extensions	1523:1532	arg1	complex					1542:1548	complex	1542:1548	complex	1542:1548	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	6	79	from	extensions	1523:1532	arg1	N-glycans					1561:1569	hybrid N-glycans	1554:1569	hybrid N-glycans	1554:1569	The database-aided glycoproteomic analyses enable rapid determination of new sulfated-N-glycan structures in large sets of influenza vaccines, including those highly branched nonsialyl sulfo-N-glycans bearing lactosaminic extensions in both complex and hybrid N-glycans that especially interact with sulfotransferases.					
30908021	1	80	theme	Sulfated	77:84	arg1	N-glycans					86:94	Sulfated N-glycans	77:94	Sulfated N-glycans	77:94	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	1	80	theme	Sulfated	77:84	arg1	structures					123:132	biologically important structures	100:132	biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	100:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	3	81	theme	chromatography-mass	518:536	arg1	spectrometry					538:549	liquid chromatography-mass spectrometry	511:549	liquid chromatography-mass spectrometry	511:549	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	0	82	theme	Structural	11:20	arg1	Diversity					22:30	Remarkable Structural Diversity	0:30	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines	0:74	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	1	83	theme	important	113:121	arg1	N-glycans					86:94	Sulfated N-glycans	77:94	Sulfated N-glycans	77:94	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	1	83	theme	important	113:121	arg1	structures					123:132	biologically important structures	100:132	biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics	100:241	Sulfated N-glycans are biologically important structures derived from enzymatically post-glycosylational modifications of glycoproteins in many therapeutic biologics.					
30908021	2	84	theme	enrichment	485:494	arg1	methods					496:502	highly selective enrichment methods	468:502	highly selective enrichment methods	468:502	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	4	85	gly	N-glycopeptides	879:893	arg2	N-glycopeptides					879:893	1380 unique N-glycopeptides	867:893	1380 unique N-glycopeptides	867:893	With the aid of database for data mining, 1380 unique N-glycopeptides were identified and manually validated by de novo glycopeptide sequencing, of which 514 were sulfated at the site-specific locations.					
30908021	7	86	theme	N-glycan	1763:1770	arg1	sulfation					1772:1780	N-glycan sulfation	1763:1780	N-glycan sulfation of influenza glycoproteins	1763:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	3	87	theme	N-glycan	730:737	arg1	library					739:745	an N-glycan library	727:745	an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions	727:822	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	0	88	theme	Influenza	57:65	arg1	Vaccines					67:74	Influenza Vaccines	57:74	Influenza Vaccines	57:74	Remarkable Structural Diversity of N-Glycan Sulfation on Influenza Vaccines.					
30908021	5	89	theme	sulfated-6-GlcNAc	1259:1275	arg1	GalNAc-GlcNAc					1226:1238	GalNAc-GlcNAc	1226:1238	GalNAc-GlcNAc	1226:1238	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	5	89	theme	sulfated-6-GlcNAc	1259:1275	arg1	Gal-GlcNAc					1211:1220	Gal-GlcNAc	1211:1220	Gal-GlcNAc	1211:1220	We report here a mass spectrometric method that is able to identify and distinguish the isobaric structures of complex and hybrid N-glycans flanked by a terminal sulfation sequon on Gal-GlcNAc and GalNAc-GlcNAc of sulfated-3-Gal, sulfated-6-GlcNAc, and sulfated-4-GalNAc.					
30908021	7	90	theme	potential	1728:1736	arg1	importance					1749:1758	potential functional importance	1728:1758	potential functional importance of N-glycan sulfation of influenza glycoproteins	1728:1807	The novel findings highlight the tremendous structural diversity of sulfated N-glycans and strongly suggest potential functional importance of N-glycan sulfation of influenza glycoproteins.					
30908021	3	91	theme	927	761:763	arg1	structures					774:783	927 N-glycan structures	761:783	927 N-glycan structures	761:783	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	2	92	theme	molecular	399:407	arg1	structures					409:418	large molecular structures	393:418	large molecular structures	393:418	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	3	93	theme	N-glycan	802:809	arg1	compositions					811:822	387 sulfated N-glycan compositions	789:822	387 sulfated N-glycan compositions	789:822	Using liquid chromatography-mass spectrometry, we have analyzed the N-glycans of influenza viral hemagglutinin and neuraminidase from several subtypes of influenza vaccines, and utilized the existing resource to establish an N-glycan library consisting of 927 N-glycan structures and 387 sulfated N-glycan compositions.					
30908021	2	94	theme	attachments	441:451	arg1	lack					460:463	a lack	458:463	a lack of highly selective enrichment methods	458:502	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	94	theme	attachments	441:451	arg1	composition					380:390	negatively charged heterogeneous composition	347:390	negatively charged heterogeneous composition	347:390	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	94	theme	attachments	441:451	arg1	lability					421:428	lability	421:428	lability of sulfate attachments	421:451	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
30908021	2	94	theme	attachments	441:451	arg1	structures					409:418	large molecular structures	393:418	large molecular structures	393:418	The high-throughput analysis of sulfated N-glycomes remains a daunting technical challenge, because of negatively charged heterogeneous composition, large molecular structures, lability of sulfate attachments, and a lack of highly selective enrichment methods.					
31813565	8	0	theme	promising	1618:1626	arg1	application					1628:1638	the promising application	1614:1638	the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples	1614:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	6	1	theme	human	1277:1281	arg1	digests					1287:1293	tryptic HRP and human IgG digests	1261:1293	digests	1287:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	3	2	theme	MOF	585:587	arg1	shell					589:593	a Zr-based MOF shell	574:593	a Zr-based MOF shell	574:593	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	2	3	dep	nanoparticles	397:409	arg1	denoted					412:418	denoted	412:418	denoted as magMOF@Au-maltose	412:439	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	2	4	theme	maltose-functionalized	326:347	arg1	framework					372:380	hydrophilic maltose-functionalized magnetic metal-organic framework	314:380	hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides	314:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	7	5	theme	N-glycosylation	1457:1471	arg1	sites					1473:1477	123 unique N-glycosylation sites	1446:1477	123 unique N-glycosylation sites	1446:1477	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	3	6	theme	resulting	600:608	arg1	MOF					610:612	the resulting MOF	596:612	the resulting MOF	596:612	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	8	7	theme	glycopeptides	1723:1735	arg1	detection					1668:1676	detection	1668:1676	detection	1668:1676	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	8	7	theme	glycopeptides	1723:1735	arg1	identification					1682:1695	identification	1682:1695	identification	1682:1695	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	1	8	theme	selective	202:210	arg1	platforms					260:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	7	9	theme	complex	1404:1410	arg1	samples					1423:1429	the complex biological samples	1400:1429	the complex biological samples	1400:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	3	10	theme	Au	635:636	arg1	nanoparticles					638:650	Au nanoparticles	635:650	Au nanoparticles	635:650	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	8	11	theme	complex	1740:1746	arg1	samples					1759:1765	complex biological samples	1740:1765	complex biological samples	1740:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	1	12	theme	efficient	216:224	arg1	platforms					260:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	1	13	theme	Biomedical	121:130	arg1	sciences					132:139	Biomedical sciences	121:139	Biomedical sciences	121:139	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	2	14	theme	metal-organic	358:370	arg1	framework					372:380	hydrophilic maltose-functionalized magnetic metal-organic framework	314:380	hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides	314:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	1	15	theme	enrichment	249:258	arg1	platforms					260:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	7	16	gly	glycopeptides	1381:1393	arg2	glycopeptides					1381:1393	glycopeptides	1381:1393	glycopeptides from the complex biological samples	1381:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	7	16	gly	glycopeptides	1381:1393	arg1	samples					1423:1429	the complex biological samples	1400:1429	the complex biological samples	1400:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	5	17	theme	magMOF	891:896	arg1	Au-maltose					898:907	magMOF@Au-maltose	891:907	magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	891:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	18	theme	outstanding	924:934	arg1	performance					936:946	the outstanding performance	920:946	the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	920:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	7	19	theme	selective	1357:1365	arg1	enrichment					1367:1376	the selective enrichment	1353:1376	the selective enrichment of glycopeptides from the complex biological samples	1353:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	8	20	from	application	1628:1638	arg1	detection					1668:1676	detection	1668:1676	detection	1668:1676	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	8	20	from	application	1628:1638	arg1	identification					1682:1695	identification	1682:1695	identification	1682:1695	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	8	21	from	detection	1668:1676	arg1	samples					1759:1765	complex biological samples	1740:1765	complex biological samples	1740:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	3	22	theme	thiol-functional	683:698	arg1	maltose					700:706	thiol-functional maltose	683:706	thiol-functional maltose	683:706	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	6	23	gly	glycopeptides	1242:1254	arg1	digests					1287:1293	tryptic HRP and human IgG digests	1261:1293	digests	1287:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	6	23	gly	glycopeptides	1242:1254	arg1	HRP					1269:1271	tryptic HRP and human IgG digests	1261:1293	HRP	1269:1271	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	6	23	gly	glycopeptides	1242:1254	arg2	glycopeptides					1242:1254	24 and 32 glycopeptides	1232:1254	24 and 32 glycopeptides from tryptic HRP and human IgG digests	1232:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	7	24	gly	glycopeptides	1504:1516	arg2	glycopeptides					1504:1516	113 glycopeptides	1500:1516	113 glycopeptides	1500:1516	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	5	25	theme	mass	1006:1009	arg1	1:200					999:1003	1:200	999:1003	1:200	999:1003	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	25	theme	mass	1006:1009	arg1	ratio					1011:1015	mass ratio	1006:1015	mass ratio of horseradish peroxidase to bovine serum albumin digest	1006:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	6	26	theme	@	1195:1195	arg1	nanocomposite					1207:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	5	27	theme	@	897:897	arg1	Au-maltose					898:907	magMOF@Au-maltose	891:907	magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	891:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	28	from	performance	936:946	arg1	glycopeptides					951:963	glycopeptides	951:963	glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	951:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	29	theme	high	981:984	arg1	selectivity					986:996	high selectivity	981:996	high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest)	981:1073	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	7	30	from	samples	1423:1429	arg1	enrichment					1367:1376	the selective enrichment	1353:1376	the selective enrichment of glycopeptides from the complex biological samples	1353:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	7	30	from	samples	1423:1429	arg1	glycopeptides					1381:1393	glycopeptides	1381:1393	glycopeptides from the complex biological samples	1381:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	0	31	theme	N-linked	97:104	arg1	glycopeptides					106:118	N-linked glycopeptides	97:118	N-linked glycopeptides	97:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	8	32	from	identification	1682:1695	arg1	samples					1759:1765	complex biological samples	1740:1765	complex biological samples	1740:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	4	33	theme	Au-maltose	825:834	arg1	adsorption					804:813	adsorption	804:813	adsorption of magMOF@Au-maltose to glycopeptides	804:851	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	4	33	theme	Au-maltose	825:834	arg1	property					791:798	The physical property	778:798	The physical property	778:798	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	5	34	theme	serum	1053:1057	arg1	albumin					1059:1065	bovine serum albumin	1046:1065	bovine serum albumin digest	1046:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	7	35	gly	N-glycosylation	1457:1471	arg2	sites					1473:1477	123 unique N-glycosylation sites	1446:1477	123 unique N-glycosylation sites	1446:1477	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	7	35	gly	N-glycosylation	1457:1471	arg2	123					1446:1448	123	1446:1448	123	1446:1448	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	1	36	theme	disease	160:166	arg1	research					178:185	particular disease biomarker research	149:185	particular disease biomarker research	149:185	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	2	37	theme	efficient	453:461	arg1	enrichment					463:472	highly efficient enrichment	446:472	highly efficient enrichment of N-linked glycopeptides	446:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	0	38	gly	glycopeptides	106:118	arg2	glycopeptides					106:118	N-linked glycopeptides	97:118	N-linked glycopeptides	97:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	5	39	dep	selectivity	986:996	arg1	1:200					999:1003	1:200	999:1003	1:200	999:1003	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	39	dep	selectivity	986:996	arg1	ratio					1011:1015	mass ratio	1006:1015	mass ratio of horseradish peroxidase to bovine serum albumin digest	1006:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	40	theme	horseradish	1020:1030	arg1	peroxidase					1032:1041	horseradish peroxidase	1020:1041	horseradish peroxidase	1020:1041	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	4	41	theme	magMOF	818:823	arg1	Au-maltose					825:834	magMOF@Au-maltose	818:834	magMOF@Au-maltose	818:834	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	2	42	theme	glycopeptides	486:498	arg1	enrichment					463:472	highly efficient enrichment	446:472	highly efficient enrichment of N-linked glycopeptides	446:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	0	43	theme	magnetic	29:36	arg1	framework					52:60	magnetic metal-organic framework	29:60	magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides	29:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	8	44	link	N-linked	1714:1721	arg1	glycopeptides					1723:1735	low-abundance N-linked glycopeptides	1700:1735	low-abundance N-linked glycopeptides	1700:1735	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	7	45	theme	human	1529:1533	arg1	serum					1535:1539	human serum	1529:1539	human serum	1529:1539	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	5	46	theme	binding	1154:1160	arg1	capacity					1162:1169	a large binding capacity	1146:1169	a large binding capacity (83 μg•mg-1)	1146:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	46	theme	binding	1154:1160	arg1	μg•mg-1					1175:1181	83 μg•mg-1	1172:1181	83 μg•mg-1	1172:1181	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	3	47	theme	@	739:739	arg1	Au-maltose					740:749	magMOF@Au-maltose	733:749	magMOF@Au-maltose with core-shell structure	733:775	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	8	48	theme	magMOF	1643:1648	arg1	Au-maltose					1650:1659	magMOF@Au-maltose	1643:1659	magMOF@Au-maltose	1643:1659	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	2	49	link	N-linked	477:484	arg1	glycopeptides					486:498	N-linked glycopeptides	477:498	N-linked glycopeptides	477:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	8	50	theme	Au-maltose	1650:1659	arg1	application					1628:1638	the promising application	1614:1638	the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples	1614:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	5	51	theme	low	1078:1080	arg1	fmol					1105:1108	10 fmol	1102:1108	10 fmol	1102:1108	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	51	theme	low	1078:1080	arg1	limit					1082:1086	a low limit	1076:1086	a low limit of detection (10 fmol)	1076:1109	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	6	52	theme	IgG	1283:1285	arg1	digests					1287:1293	tryptic HRP and human IgG digests	1261:1293	digests	1287:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	2	53	theme	magMOF	423:428	arg1	Au-maltose					430:439	magMOF@Au-maltose	423:439	magMOF@Au-maltose	423:439	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	7	54	theme	sites	1473:1477	arg1	total					1437:1441	a total	1435:1441	a total of 123 unique N-glycosylation sites	1435:1477	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	3	55	dep	brief	504:508	arg1	carboxyl-functional					511:529	carboxyl-functional	511:529	carboxyl-functional	511:529	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	2	56	theme	magnetic	349:356	arg1	framework					372:380	hydrophilic maltose-functionalized magnetic metal-organic framework	314:380	hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides	314:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	7	57	theme	unique	1450:1455	arg1	sites					1473:1477	123 unique N-glycosylation sites	1446:1477	123 unique N-glycosylation sites	1446:1477	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	5	58	gly	glycopeptides	951:963	arg2	glycopeptides					951:963	glycopeptides	951:963	glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	951:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	6	59	theme	tryptic	1261:1267	arg1	HRP					1269:1271	tryptic HRP and human IgG digests	1261:1293	HRP	1269:1271	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	2	60	theme	hydrophilic	314:324	arg1	framework					372:380	hydrophilic maltose-functionalized magnetic metal-organic framework	314:380	hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides	314:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	7	61	gly	glycoproteins	1578:1590	arg1	glycoproteins					1578:1590	46 different glycoproteins	1565:1590	46 different glycoproteins	1565:1590	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	4	62	gly	glycopeptides	839:851	arg2	glycopeptides					839:851	glycopeptides	839:851	glycopeptides	839:851	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	8	63	theme	N-linked	1714:1721	arg1	glycopeptides					1723:1735	low-abundance N-linked glycopeptides	1700:1735	low-abundance N-linked glycopeptides	1700:1735	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	7	64	theme	biological	1412:1421	arg1	samples					1423:1429	the complex biological samples	1400:1429	the complex biological samples	1400:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	1	65	theme	glycoproteins/peptides	226:247	arg1	platforms					260:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	highly selective and efficient glycoproteins/peptides enrichment platforms	195:268	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	3	66	theme	Zr-based	576:583	arg1	shell					589:593	a Zr-based MOF shell	574:593	a Zr-based MOF shell	574:593	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	5	67	theme	high	1114:1117	arg1	recovery					1119:1126	a high recovery	1112:1126	a high recovery (over 83.3%)	1112:1139	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	68	theme	enrichment	965:974	arg1	glycopeptides					951:963	glycopeptides	951:963	glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	951:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	8	69	theme	@	1649:1649	arg1	Au-maltose					1650:1659	magMOF@Au-maltose	1643:1659	magMOF@Au-maltose	1643:1659	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	8	70	gly	glycopeptides	1723:1735	arg2	glycopeptides					1723:1735	low-abundance N-linked glycopeptides	1700:1735	low-abundance N-linked glycopeptides	1700:1735	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	7	71	theme	glycopeptides	1381:1393	arg1	enrichment					1367:1376	the selective enrichment	1353:1376	the selective enrichment of glycopeptides from the complex biological samples	1353:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	8	72	dep	detection	1668:1676	arg1	the					1664:1666	the	1664:1666	the	1664:1666	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	1	73	theme	particular	149:158	arg1	research					178:185	particular disease biomarker research	149:185	particular disease biomarker research	149:185	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	7	74	theme	different	1568:1576	arg1	glycoproteins					1578:1590	46 different glycoproteins	1565:1590	46 different glycoproteins	1565:1590	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	6	75	theme	Au-maltose	1196:1205	arg1	nanocomposite					1207:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	4	76	theme	physical	782:789	arg1	property					791:798	The physical property	778:798	The physical property	778:798	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	2	77	theme	facile	287:292	arg1	strategy					294:301	a facile strategy	285:301	a facile strategy	285:301	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	1	78	theme	biomarker	168:176	arg1	research					178:185	particular disease biomarker research	149:185	particular disease biomarker research	149:185	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	6	79	theme	magMOF	1189:1194	arg1	nanocomposite					1207:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite	1185:1219	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	0	80	theme	glycopeptides	106:118	arg1	enrichment					83:92	highly efficient enrichment	66:92	highly efficient enrichment of N-linked glycopeptides	66:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	2	81	gly	glycopeptides	486:498	arg2	glycopeptides					486:498	N-linked glycopeptides	477:498	N-linked glycopeptides	477:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	5	82	theme	large	1148:1152	arg1	capacity					1162:1169	a large binding capacity	1146:1169	a large binding capacity (83 μg•mg-1)	1146:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	82	theme	large	1148:1152	arg1	μg•mg-1					1175:1181	83 μg•mg-1	1172:1181	83 μg•mg-1	1172:1181	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	83	theme	bovine	1046:1051	arg1	albumin					1059:1065	bovine serum albumin	1046:1065	bovine serum albumin digest	1046:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	4	84	theme	@	824:824	arg1	Au-maltose					825:834	magMOF@Au-maltose	818:834	magMOF@Au-maltose	818:834	The physical property and adsorption of magMOF@Au-maltose to glycopeptides were investigated.					
31813565	5	85	theme	albumin	1059:1065	arg1	digest					1067:1072	bovine serum albumin digest	1046:1072	bovine serum albumin digest	1046:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	86	contain	possessing	909:918	arg1	Au-maltose					898:907	magMOF@Au-maltose	891:907	magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	891:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	86	contain	possessing	909:918	arg2	performance					936:946	the outstanding performance	920:946	the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1)	920:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	0	87	theme	metal-organic	38:50	arg1	framework					52:60	magnetic metal-organic framework	29:60	magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides	29:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	5	88	theme	peroxidase	1032:1041	arg1	1:200					999:1003	1:200	999:1003	1:200	999:1003	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	88	theme	peroxidase	1032:1041	arg1	ratio					1011:1015	mass ratio	1006:1015	mass ratio of horseradish peroxidase to bovine serum albumin digest	1006:1072	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	7	89	from	enrichment	1367:1376	arg1	samples					1423:1429	the complex biological samples	1400:1429	the complex biological samples	1400:1429	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	3	90	theme	Au-S	712:715	arg1	bonds					717:721	Au-S bonds to obtain magMOF@Au-maltose with core-shell structure	712:775	Au-S bonds to obtain magMOF@Au-maltose with core-shell structure	712:775	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	8	91	theme	biological	1748:1757	arg1	samples					1759:1765	complex biological samples	1740:1765	complex biological samples	1740:1765	These results showed the promising application of magMOF@Au-maltose in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.					
31813565	2	92	theme	N-linked	477:484	arg1	glycopeptides					486:498	N-linked glycopeptides	477:498	N-linked glycopeptides	477:498	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	7	93	theme	serum	1535:1539	arg1	μL					1523:1524	1 μL	1521:1524	1 μL	1521:1524	Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins.					
31813565	3	94	theme	brief	504:508	arg1	nanospheres					537:547	brief, carboxyl-functional Fe3O4 nanospheres	504:547	brief, carboxyl-functional Fe3O4 nanospheres	504:547	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	3	95	theme	Fe3O4	531:535	arg1	nanospheres					537:547	brief, carboxyl-functional Fe3O4 nanospheres	504:547	brief, carboxyl-functional Fe3O4 nanospheres	504:547	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	0	96	theme	efficient	73:81	arg1	enrichment					83:92	highly efficient enrichment	66:92	highly efficient enrichment of N-linked glycopeptides	66:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	6	97	from	digests	1287:1293	arg1	glycopeptides					1242:1254	24 and 32 glycopeptides	1232:1254	24 and 32 glycopeptides from tryptic HRP and human IgG digests	1232:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	5	98	theme	detection	1091:1099	arg1	fmol					1105:1108	10 fmol	1102:1108	10 fmol	1102:1108	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	98	theme	detection	1091:1099	arg1	μg•mg-1					1175:1181	83 μg•mg-1	1172:1181	83 μg•mg-1	1172:1181	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	98	theme	detection	1091:1099	arg1	limit					1082:1086	a low limit	1076:1086	a low limit of detection (10 fmol)	1076:1109	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	98	theme	detection	1091:1099	arg1	selectivity					986:996	high selectivity	981:996	high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest)	981:1073	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	98	theme	detection	1091:1099	arg1	capacity					1162:1169	a large binding capacity	1146:1169	a large binding capacity (83 μg•mg-1)	1146:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	98	theme	detection	1091:1099	arg1	recovery					1119:1126	a high recovery	1112:1126	a high recovery (over 83.3%)	1112:1139	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	0	99	link	N-linked	97:104	arg1	glycopeptides					106:118	N-linked glycopeptides	97:118	N-linked glycopeptides	97:118	Hydrophilic maltose-modified magnetic metal-organic framework for highly efficient enrichment of N-linked glycopeptides.					
31813565	5	100	with	enrichment	965:974	arg1	fmol					1105:1108	10 fmol	1102:1108	10 fmol	1102:1108	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	100	with	enrichment	965:974	arg1	μg•mg-1					1175:1181	83 μg•mg-1	1172:1181	83 μg•mg-1	1172:1181	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	100	with	enrichment	965:974	arg1	limit					1082:1086	a low limit	1076:1086	a low limit of detection (10 fmol)	1076:1109	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	100	with	enrichment	965:974	arg1	selectivity					986:996	high selectivity	981:996	high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest)	981:1073	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	100	with	enrichment	965:974	arg1	capacity					1162:1169	a large binding capacity	1146:1169	a large binding capacity (83 μg•mg-1)	1146:1182	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	5	100	with	enrichment	965:974	arg1	recovery					1119:1126	a high recovery	1112:1126	a high recovery (over 83.3%)	1112:1139	The results showed magMOF@Au-maltose possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg-1).					
31813565	3	101	theme	magMOF	733:738	arg1	Au-maltose					740:749	magMOF@Au-maltose	733:749	magMOF@Au-maltose with core-shell structure	733:775	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	1	102	from	sciences	132:139	arg1	research					178:185	particular disease biomarker research	149:185	particular disease biomarker research	149:185	Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms.					
31813565	2	103	theme	Au	394:395	arg1	nanoparticles					397:409	Au nanoparticles	394:409	Au nanoparticles (denoted as magMOF@Au-maltose)	394:440	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	6	104	from	HRP	1269:1271	arg1	glycopeptides					1242:1254	24 and 32 glycopeptides	1232:1254	24 and 32 glycopeptides from tryptic HRP and human IgG digests	1232:1293	The magMOF@Au-maltose nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively.					
31813565	3	105	with	Au-maltose	740:749	arg1	structure					767:775	core-shell structure	756:775	core-shell structure	756:775	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	3	106	theme	core-shell	756:765	arg1	structure					767:775	core-shell structure	756:775	core-shell structure	756:775	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	2	107	theme	@	429:429	arg1	Au-maltose					430:439	magMOF@Au-maltose	423:439	magMOF@Au-maltose	423:439	In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as magMOF@Au-maltose) for highly efficient enrichment of N-linked glycopeptides.					
31813565	3	108	mod	modified	669:676	arg1	MOF					610:612	the resulting MOF	596:612	the resulting MOF	596:612	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31813565	3	108	mod	modified	669:676	arg3	maltose					700:706	thiol-functional maltose	683:706	thiol-functional maltose	683:706	In brief, carboxyl-functional Fe3O4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au-S bonds to obtain magMOF@Au-maltose with core-shell structure.					
31276431	5	0	theme	quality	773:779	arg1	attributes					781:790	Fc-glycosylation critical quality attributes	747:790	Fc-glycosylation critical quality attributes related to ADCC	747:806	Thus, it is highly relevant for assessing Fc-glycosylation critical quality attributes related to ADCC.					
31276431	1	1	from	cytotoxicity	205:216	arg1	affinity					145:152	FcɣRIIIa affinity	136:152	FcɣRIIIa affinity	136:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	4	2	theme	relationships	690:702	arg1	understanding					633:645	the understanding	629:645	the understanding of individual glycoform structure-function relationships	629:702	Our method greatly improves the understanding of individual glycoform structure-function relationships.					
31276431	1	3	gly	glycoforms	122:131	arg1	antibody					113:120	individual antibody glycoforms	102:131	individual antibody glycoforms	102:131	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	5	4	theme	related	792:798	arg1	attributes					781:790	Fc-glycosylation critical quality attributes	747:790	Fc-glycosylation critical quality attributes related to ADCC	747:806	Thus, it is highly relevant for assessing Fc-glycosylation critical quality attributes related to ADCC.					
31276431	2	5	theme	direct	358:363	arg1	comparison					374:383	direct affinity comparison	358:383	direct affinity comparison of glycoforms of intact monoclonal antibodies	358:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	1	6	from	impact	92:97	arg1	affinity					145:152	FcɣRIIIa affinity	136:152	FcɣRIIIa affinity	136:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	2	7	theme	glycoforms	388:397	arg1	comparison					374:383	direct affinity comparison	358:383	direct affinity comparison of glycoforms of intact monoclonal antibodies	358:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	3	8	dep	low-abundant	565:576	arg1	unstudied					579:587	unstudied	579:587	unstudied	579:587	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	1	9	theme	impact	92:97	arg1	cytotoxicity					205:216	antibody-dependent cell-mediated cytotoxicity	172:216	antibody-dependent cell-mediated cytotoxicity (ADCC)	172:223	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	9	theme	impact	92:97	arg1	ADCC					219:222	ADCC	219:222	ADCC	219:222	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	9	theme	impact	92:97	arg1	Determination					71:83	Determination	71:83	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity	71:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	10	from	Determination	71:83	arg1	affinity					145:152	FcɣRIIIa affinity	136:152	FcɣRIIIa affinity	136:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	11	theme	individual	102:111	arg1	glycoforms					122:131	individual antibody glycoforms	102:131	individual antibody glycoforms	102:131	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	3	12	theme	afucosylation	529:541	arg1	pairing					543:549	afucosylation pairing	529:549	afucosylation pairing	529:549	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	0	13	theme	FcɣRIIIa	19:26	arg1	spectrometry					57:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry	0:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.	0:69	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.					
31276431	5	14	theme	Fc-glycosylation	747:762	arg1	attributes					781:790	Fc-glycosylation critical quality attributes	747:790	Fc-glycosylation critical quality attributes related to ADCC	747:806	Thus, it is highly relevant for assessing Fc-glycosylation critical quality attributes related to ADCC.					
31276431	1	15	theme	antibody	113:120	arg1	glycoforms					122:131	individual antibody glycoforms	102:131	individual antibody glycoforms	102:131	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	0	16	theme	Glycoform-resolved	0:17	arg1	spectrometry					57:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry	0:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.	0:69	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.					
31276431	4	17	theme	individual	650:659	arg1	relationships					690:702	individual glycoform structure-function relationships	650:702	individual glycoform structure-function relationships	650:702	Our method greatly improves the understanding of individual glycoform structure-function relationships.					
31276431	5	18	theme	critical	764:771	arg1	attributes					781:790	Fc-glycosylation critical quality attributes	747:790	Fc-glycosylation critical quality attributes related to ADCC	747:806	Thus, it is highly relevant for assessing Fc-glycosylation critical quality attributes related to ADCC.					
31276431	1	19	from	affinity	145:152	arg1	cytotoxicity					205:216	antibody-dependent cell-mediated cytotoxicity	172:216	antibody-dependent cell-mediated cytotoxicity (ADCC)	172:223	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	19	from	affinity	145:152	arg1	ADCC					219:222	ADCC	219:222	ADCC	219:222	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	19	from	affinity	145:152	arg1	Determination					71:83	Determination	71:83	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity	71:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	20	theme	glycoforms	122:131	arg1	impact					92:97	the impact	88:97	the impact of individual antibody glycoforms on FcɣRIIIa affinity	88:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	0	21	theme	chromatography-mass	37:55	arg1	spectrometry					57:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry	0:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.	0:69	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.					
31276431	1	22	theme	high	245:248	arg1	glycoengineering					257:272	high purity glycoengineering	245:272	high purity glycoengineering	245:272	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	0	23	theme	affinity	28:35	arg1	spectrometry					57:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry	0:68	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.	0:69	Glycoform-resolved FcɣRIIIa affinity chromatography-mass spectrometry.					
31276431	4	24	theme	structure-function	671:688	arg1	relationships					690:702	individual glycoform structure-function relationships	650:702	individual glycoform structure-function relationships	650:702	Our method greatly improves the understanding of individual glycoform structure-function relationships.					
31276431	1	25	theme	FcɣRIIIa	136:143	arg1	affinity					145:152	FcɣRIIIa affinity	136:152	FcɣRIIIa affinity	136:152	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	26	theme	purity	250:255	arg1	glycoengineering					257:272	high purity glycoengineering	245:272	high purity glycoengineering	245:272	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	3	27	theme	effects	504:510	arg1	refinement					470:479	refinement	470:479	refinement	470:479	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	3	27	theme	effects	504:510	arg1	reproduction					453:464	reproduction	453:464	reproduction	453:464	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	4	28	theme	glycoform	661:669	arg1	relationships					690:702	individual glycoform structure-function relationships	650:702	individual glycoform structure-function relationships	650:702	Our method greatly improves the understanding of individual glycoform structure-function relationships.					
31276431	2	29	theme	affinity	298:305	arg1	chromatography					307:320	FcɣRIIIa affinity chromatography	289:320	FcɣRIIIa affinity chromatography	289:320	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	2	30	theme	FcɣRIIIa	289:296	arg1	chromatography					307:320	FcɣRIIIa affinity chromatography	289:320	FcɣRIIIa affinity chromatography	289:320	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	3	31	theme	known	484:488	arg1	effects					504:510	known glycosylation effects	484:510	known glycosylation effects	484:510	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	2	32	theme	antibodies	420:429	arg1	glycoforms					388:397	glycoforms	388:397	glycoforms of intact monoclonal antibodies	388:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	3	33	theme	glycosylation	490:502	arg1	effects					504:510	known glycosylation effects	484:510	known glycosylation effects	484:510	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	2	34	theme	monoclonal	409:418	arg1	antibodies					420:429	intact monoclonal antibodies	402:429	intact monoclonal antibodies	402:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	2	35	theme	affinity	365:372	arg1	comparison					374:383	direct affinity comparison	358:383	direct affinity comparison of glycoforms of intact monoclonal antibodies	358:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	2	36	theme	intact	402:407	arg1	antibodies					420:429	intact monoclonal antibodies	402:429	intact monoclonal antibodies	402:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	2	37	theme	mass	325:328	arg1	spectrometry					330:341	mass spectrometry	325:341	mass spectrometry	325:341	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	3	38	theme	low-abundant	565:576	arg1	glycoforms					589:598	low-abundant, unstudied glycoforms	565:598	low-abundant, unstudied glycoforms	565:598	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	3	39	from	insights	517:524	arg1	glycoforms					589:598	low-abundant, unstudied glycoforms	565:598	low-abundant, unstudied glycoforms	565:598	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	3	39	from	insights	517:524	arg1	pairing					543:549	afucosylation pairing	529:549	afucosylation pairing	529:549	The approach enabled reproduction and refinement of known glycosylation effects, and insights on afucosylation pairing as well as on low-abundant, unstudied glycoforms.					
31276431	1	40	theme	antibody-dependent	172:189	arg1	ADCC					219:222	ADCC	219:222	ADCC	219:222	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	40	theme	antibody-dependent	172:189	arg1	cytotoxicity					205:216	antibody-dependent cell-mediated cytotoxicity	172:216	antibody-dependent cell-mediated cytotoxicity (ADCC)	172:223	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	2	41	gly	glycoforms	388:397	arg1	antibodies					420:429	intact monoclonal antibodies	402:429	intact monoclonal antibodies	402:429	We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affinity comparison of glycoforms of intact monoclonal antibodies.					
31276431	1	42	theme	cell-mediated	191:203	arg1	ADCC					219:222	ADCC	219:222	ADCC	219:222	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
31276431	1	42	theme	cell-mediated	191:203	arg1	cytotoxicity					205:216	antibody-dependent cell-mediated cytotoxicity	172:216	antibody-dependent cell-mediated cytotoxicity (ADCC)	172:223	Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.					
29595997	2	0	theme	protective	444:453	arg1	barrier					455:461	a protective barrier	442:461	a protective barrier	442:461	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	5	1	theme	robust	867:872	arg1	methods					886:892	robust biophysical methods	867:892	robust biophysical methods	867:892	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	2	from	structure	935:943	arg1	strategies					1023:1032	vaccine design strategies	1008:1032	vaccine design strategies that takes into account the essential glycan components	1008:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	3	theme	innovation	994:1003	arg1	function					949:956	function	949:956	function	949:956	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	3	theme	innovation	994:1003	arg1	structure					935:943	structure	935:943	structure	935:943	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	4	theme	structure	935:943	arg1	understanding					914:926	transformed our understanding	898:926	transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	898:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	5	theme	essential	1062:1070	arg1	components					1079:1088	the essential glycan components	1058:1088	account the essential glycan components	1050:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	4	6	theme	complex	723:729	arg1	structure					731:739	the complex structure	719:739	the complex structure of the Env-spike glycoprotein	719:769	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	5	7	theme	transformed	898:908	arg1	understanding					914:926	transformed our understanding	898:926	transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	898:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	2	8	theme	Env	470:472	arg1	protein					474:480	the Env protein	466:480	the Env protein	466:480	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	5	9	theme	glycan	1072:1077	arg1	components					1079:1088	the essential glycan components	1058:1088	account the essential glycan components	1050:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	4	10	theme	protein	807:813	arg1	epitopes					815:822	glycan and protein epitopes	796:822	epitopes	815:822	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	1	11	theme	Vaccine	59:65	arg1	efforts					74:80	Vaccine design efforts	59:80	Vaccine design efforts against the human immunodeficiency virus (HIV)	59:127	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	2	12	theme	viral	373:377	arg1	protein					388:394	viral envelope protein	373:394	the viral envelope protein (Env)	369:400	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	2	12	theme	viral	373:377	arg1	Env					397:399	Env	397:399	Env	397:399	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	2	13	theme	numerous	415:422	arg1	glycans					424:430	the numerous glycans	411:430	not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein	330:480	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	1	14	theme	design	67:72	arg1	efforts					74:80	Vaccine design efforts	59:80	Vaccine design efforts against the human immunodeficiency virus (HIV)	59:127	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	15	theme	infected	187:194	arg1	patients					196:203	many infected patients	182:203	many infected patients	182:203	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	4	16	theme	epitopes	815:822	arg1	combination					781:791	its combination	777:791	its combination of glycan and protein epitopes	777:822	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	3	17	theme	host	531:534	arg1	glycoproteins					536:548	host glycoproteins	531:548	host glycoproteins	531:548	Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response.					
29595997	5	18	from	innovation	994:1003	arg1	strategies					1023:1032	vaccine design strategies	1008:1032	vaccine design strategies that takes into account the essential glycan components	1008:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	4	19	theme	considerable	621:632	arg1	efforts					634:640	considerable efforts	621:640	considerable efforts	621:640	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	0	20	theme	Immune	14:19	arg1	Recognition					21:31	Immune Recognition	14:31	Immune Recognition	14:31	Structure and Immune Recognition of the HIV Glycan Shield.					
29595997	5	21	theme	function	949:956	arg1	understanding					914:926	transformed our understanding	898:926	transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	898:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	1	22	theme	human	94:98	arg1	HIV					124:126	HIV	124:126	HIV	124:126	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	22	theme	human	94:98	arg1	virus					117:121	the human immunodeficiency virus	90:121	the human immunodeficiency virus (HIV)	90:127	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	23	theme	many	182:185	arg1	patients					196:203	many infected patients	182:203	many infected patients	182:203	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	24	dep	potent	231:236	arg1	neutralizing					246:257	neutralizing	246:257	neutralizing	246:257	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	2	25	theme	protein	388:394	arg1	glycans					424:430	the numerous glycans	411:430	not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein	330:480	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	2	25	theme	protein	388:394	arg1	components					355:364	the two protein components	339:364	not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein	330:480	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	4	26	gly	glycoprotein	758:769	arg1	glycoprotein					758:769	the Env-spike glycoprotein	744:769	the Env-spike glycoprotein	744:769	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	5	27	theme	stimulated	983:992	arg1	innovation					994:1003	the HIV Env spike and stimulated innovation	961:1003	the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	961:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	3	28	gly	glycoproteins	536:548	arg1	glycoproteins					536:548	host glycoproteins	531:548	host glycoproteins	531:548	Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response.					
29595997	0	29	theme	Glycan	44:49	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Immune Recognition of the HIV Glycan Shield.					
29595997	0	29	theme	Glycan	44:49	arg1	Recognition					21:31	Immune Recognition	14:31	Immune Recognition	14:31	Structure and Immune Recognition of the HIV Glycan Shield.					
29595997	3	30	theme	antibody	591:598	arg1	response					600:607	the antibody response	587:607	the antibody response	587:607	Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response.					
29595997	4	31	theme	glycan	796:801	arg1	epitopes					815:822	glycan and protein epitopes	796:822	epitopes	815:822	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	5	32	contain	has	894:896	arg1	application					852:862	the application	848:862	the application of robust biophysical methods	848:892	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	32	contain	has	894:896	arg2	understanding					914:926	transformed our understanding	898:926	transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	898:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	1	33	theme	immunodeficiency	100:115	arg1	HIV					124:126	HIV	124:126	HIV	124:126	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	33	theme	immunodeficiency	100:115	arg1	virus					117:121	the human immunodeficiency virus	90:121	the human immunodeficiency virus (HIV)	90:127	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	0	34	theme	HIV	40:42	arg1	Glycan					44:49	the HIV Glycan	36:49	the HIV Glycan	36:49	Structure and Immune Recognition of the HIV Glycan Shield.					
29595997	5	35	theme	vaccine	1008:1014	arg1	strategies					1023:1032	vaccine design strategies	1008:1032	vaccine design strategies that takes into account the essential glycan components	1008:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	36	theme	methods	886:892	arg1	application					852:862	the application	848:862	the application of robust biophysical methods	848:892	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	37	theme	design	1016:1021	arg1	strategies					1023:1032	vaccine design strategies	1008:1032	vaccine design strategies that takes into account the essential glycan components	1008:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	4	38	theme	glycoprotein	758:769	arg1	structure					731:739	the complex structure	719:739	the complex structure of the Env-spike glycoprotein	719:769	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	4	39	theme	biophysical	686:696	arg1	methods					698:704	biophysical methods	686:704	biophysical methods	686:704	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	5	40	dep	account	1050:1056	arg1	components					1079:1088	the essential glycan components	1058:1088	account the essential glycan components	1050:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	41	theme	biophysical	874:884	arg1	methods					886:892	robust biophysical methods	867:892	robust biophysical methods	867:892	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	4	42	theme	Env-spike	748:756	arg1	glycoprotein					758:769	the Env-spike glycoprotein	744:769	the Env-spike glycoprotein	744:769	Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes.					
29595997	3	43	gly	glycosylated	506:517	arg1	Env					491:493	Env	491:493	Env	491:493	Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response.					
29595997	5	44	dep	structure	935:943	arg1	the					931:933	the	931:933	the	931:933	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	45	from	strategies	1023:1032	arg1	function					949:956	function	949:956	function	949:956	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	45	from	strategies	1023:1032	arg1	structure					935:943	structure	935:943	structure	935:943	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	2	46	theme	envelope	379:386	arg1	protein					388:394	viral envelope protein	373:394	the viral envelope protein (Env)	369:400	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	2	46	theme	envelope	379:386	arg1	Env					397:399	Env	397:399	Env	397:399	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	2	47	theme	protein	347:353	arg1	components					355:364	the two protein components	339:364	not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein	330:480	Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein.					
29595997	5	48	theme	spike	973:977	arg1	innovation					994:1003	the HIV Env spike and stimulated innovation	961:1003	the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components	961:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	5	49	from	function	949:956	arg1	strategies					1023:1032	vaccine design strategies	1008:1032	vaccine design strategies that takes into account the essential glycan components	1008:1088	We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.					
29595997	1	50	theme	potent	231:236	arg1	bnAbs					271:275	bnAbs	271:275	bnAbs	271:275	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
29595997	1	50	theme	potent	231:236	arg1	antibodies					259:268	highly potent broadly neutralizing antibodies	224:268	highly potent broadly neutralizing antibodies (bnAbs)	224:276	Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs).					
30242110	4	0	theme	desorption/ionization	1042:1062	arg1	MALDI-TOF-MS					1098:1109	MALDI-TOF-MS	1098:1109	MALDI-TOF-MS	1098:1109	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	0	theme	desorption/ionization	1042:1062	arg1	spectrometry					1084:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1020:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification	1020:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	1	theme	varying	2402:2408	arg1	complexity					2410:2419	varying complexity	2402:2419	varying complexity	2402:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	6	2	theme	serum	1474:1478	arg1	changes					1504:1510	serum protein N-glycosylation changes	1474:1510	serum protein N-glycosylation changes throughout pregnancy	1474:1531	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	2	3	theme	relative	373:380	arg1	abundances					382:391	the relative abundances	369:391	the relative abundances of protein glycoforms	369:413	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	8	4	theme	compositional	1901:1913	arg1	information					1915:1925	compositional information	1901:1925	compositional information on higher-complexity N-glycans	1901:1956	MALDI-TOF-MS achieved the highest throughput and provided compositional information on higher-complexity N-glycans.					
30242110	4	5	theme	mass	1079:1082	arg1	MALDI-TOF-MS					1098:1109	MALDI-TOF-MS	1098:1109	MALDI-TOF-MS	1098:1109	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	5	theme	mass	1079:1082	arg1	spectrometry					1084:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1020:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification	1020:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	6	theme	samples	2391:2397	arg1	analysis					2360:2367	the glycosylation analysis	2342:2367	the glycosylation analysis of single proteins or samples of varying complexity	2342:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	9	7	from	differences	2125:2135	arg1	galactosylation					2162:2176	α1,3- and α1,6-branch galactosylation	2140:2176	α1,3- and α1,6-branch galactosylation	2140:2176	Consequentially, MALDI-TOF-MS could establish the linkage-specific sialylation differences within pregnancy and RA, whereas HILIC-UHPLC-FLD and xCGE-LIF demonstrated differences in α1,3- and α1,6-branch galactosylation.					
30242110	0	8	dep	N-Glycomics	22:32	arg1	N-Glycomics					22:32	High-throughput Serum N-Glycomics	0:32	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.	0:131	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	8	dep	N-Glycomics	22:32	arg1	Comparison					42:51	Method Comparison	35:51	Method Comparison	35:51	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	8	dep	N-Glycomics	22:32	arg1	Application					57:67	Application	57:67	Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes	57:130	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	1	9	gly	glycoprotein	222:233	arg1	glycoprotein					222:233	glycoprotein characteristics	222:249	glycoprotein characteristics such as serum half-life and receptor interaction	222:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	10	theme	major	206:210	arg1	impact					212:217	a major impact	204:217	a major impact on glycoprotein characteristics such as serum half-life and receptor interaction	204:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	4	11	theme	matrix-assisted	1020:1034	arg1	MALDI-TOF-MS					1098:1109	MALDI-TOF-MS	1098:1109	MALDI-TOF-MS	1098:1109	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	11	theme	matrix-assisted	1020:1034	arg1	spectrometry					1084:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1020:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification	1020:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	12	theme	protein	2277:2283	arg1	N-glycosylation					2285:2299	total serum protein N-glycosylation	2265:2299	total serum protein N-glycosylation	2265:2299	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	4	13	theme	linkage-specific	1117:1132	arg1	acid					1141:1144	linkage-specific sialic acid	1117:1144	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	14	theme	hydrophilic-interaction	707:729	arg1	chromatography					761:774	hydrophilic-interaction ultra-high-performance liquid chromatography	707:774	hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans	707:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	labeling					844:851	2-aminobenzamide labeling	827:851	2-aminobenzamide labeling of the glycans	827:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	labeling					1006:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	xCGE-LIF					954:961	xCGE-LIF	954:961	xCGE-LIF	954:961	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	HILIC-UHPLC-FLD					805:819	HILIC-UHPLC-FLD	805:819	HILIC-UHPLC-FLD	805:819	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	esterification					1146:1159	linkage-specific sialic acid esterification	1117:1159	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	detection					943:951	laser-induced fluorescence detection	916:951	laser-induced fluorescence detection (xCGE-LIF)	916:962	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	15	with	electrophoresis	895:909	arg1	detection					794:802	fluorescence detection	781:802	fluorescence detection (HILIC-UHPLC-FLD)	781:820	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	16	theme	acid	1141:1144	arg1	esterification					1146:1159	linkage-specific sialic acid esterification	1117:1159	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	3	17	theme	Several	464:470	arg1	methods					483:489	Several analytical methods	464:489	Several analytical methods	464:489	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	4	18	theme	liquid	754:759	arg1	chromatography					761:774	hydrophilic-interaction ultra-high-performance liquid chromatography	707:774	hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans	707:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	19	theme	method	2311:2316	arg1	choices					2318:2324	informed method choices	2302:2324	informed method choices	2302:2324	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	3	20	theme	N-glycome	665:673	arg1	methods					684:690	three high-throughput released N-glycome analysis methods	634:690	three high-throughput released N-glycome analysis methods	634:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	5	21	theme	multiple	1239:1246	arg1	points					1253:1258	multiple time points	1239:1258	multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1239:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	3	22	theme	high-throughput	640:654	arg1	methods					684:690	three high-throughput released N-glycome analysis methods	634:690	three high-throughput released N-glycome analysis methods	634:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	7	23	theme	N-glycans	1832:1840	arg1	separation					1803:1812	the best structural separation	1783:1812	the best structural separation of low-complexity N-glycans	1783:1840	However, the non-MS methods showed superior repeatability over MALDI-TOF-MS and allowed the best structural separation of low-complexity N-glycans.					
30242110	9	24	theme	linkage-specific	2009:2024	arg1	differences					2038:2048	the linkage-specific sialylation differences	2005:2048	the linkage-specific sialylation differences within pregnancy and RA	2005:2072	Consequentially, MALDI-TOF-MS could establish the linkage-specific sialylation differences within pregnancy and RA, whereas HILIC-UHPLC-FLD and xCGE-LIF demonstrated differences in α1,3- and α1,6-branch galactosylation.					
30242110	5	25	with	women	1317:1321	arg1	RA					1363:1364	RA	1363:1364	RA	1363:1364	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	5	25	with	women	1317:1321	arg1	arthritis					1352:1360	rheumatoid arthritis	1341:1360	rheumatoid arthritis (RA)	1341:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	6	26	theme	N-glycosylation	1488:1502	arg1	changes					1504:1510	serum protein N-glycosylation changes	1474:1510	serum protein N-glycosylation changes throughout pregnancy	1474:1531	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	27	from	similar	1606:1612	arg1	detection					1623:1631	their detection	1617:1631	their detection	1617:1631	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	27	from	similar	1606:1612	arg1	quantification					1646:1659	relative quantification	1637:1659	relative quantification	1637:1659	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	2	28	theme	More	301:304	arg1	half					311:314	More than half	301:314	More than half of the proteins in human serum	301:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	1	29	from	impact	212:217	arg1	characteristics					235:249	glycoprotein characteristics	222:249	glycoprotein characteristics such as serum half-life and receptor interaction	222:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	29	from	impact	212:217	arg1	half-life					265:273	serum half-life	259:273	serum half-life	259:273	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	29	from	impact	212:217	arg1	interaction					288:298	receptor interaction	279:298	receptor interaction	279:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	2	30	from	proteins	323:330	arg1	serum					341:345	human serum	335:345	human serum	335:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	3	31	theme	methods	684:690	arg1	performance					619:629	the performance	615:629	the performance of three high-throughput released N-glycome analysis methods	615:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	1	32	theme	protein	178:184	arg1	N-Glycosylation					133:147	N-Glycosylation	133:147	N-Glycosylation	133:147	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	32	theme	protein	178:184	arg1	modification					186:197	a fundamentally important protein modification	152:197	a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction	152:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	7	33	theme	structural	1792:1801	arg1	separation					1803:1812	the best structural separation	1783:1812	the best structural separation of low-complexity N-glycans	1783:1840	However, the non-MS methods showed superior repeatability over MALDI-TOF-MS and allowed the best structural separation of low-complexity N-glycans.					
30242110	6	34	from	detection	1623:1631	arg1	similar					1606:1612	similar	1606:1612	similar	1606:1612	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	34	from	detection	1623:1631	arg1	methods					1585:1591	the methods	1581:1591	the methods	1581:1591	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	0	35	theme	Rheumatoid	78:87	arg1	Arthritis					89:97	Study Rheumatoid Arthritis	72:97	Study Rheumatoid Arthritis	72:97	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	8	36	from	information	1915:1925	arg1	N-glycans					1948:1956	higher-complexity N-glycans	1930:1956	higher-complexity N-glycans	1930:1956	MALDI-TOF-MS achieved the highest throughput and provided compositional information on higher-complexity N-glycans.					
30242110	5	37	with	patients	1327:1334	arg1	RA					1363:1364	RA	1363:1364	RA	1363:1364	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	5	37	with	patients	1327:1334	arg1	arthritis					1352:1360	rheumatoid arthritis	1341:1360	rheumatoid arthritis (RA)	1341:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	6	38	theme	RA	1552:1553	arg1	activity.Overall					1563:1578	RA disease activity.Overall	1552:1578	RA disease activity.Overall	1552:1578	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	4	39	theme	laser-induced	916:928	arg1	xCGE-LIF					954:961	xCGE-LIF	954:961	xCGE-LIF	954:961	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	39	theme	laser-induced	916:928	arg1	detection					943:951	laser-induced fluorescence detection	916:951	laser-induced fluorescence detection (xCGE-LIF)	916:962	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	3	40	theme	analytical	472:481	arg1	methods					483:489	Several analytical methods	464:489	Several analytical methods	464:489	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	5	41	theme	healthy	1309:1315	arg1	women					1317:1321	healthy women	1309:1321	healthy women	1309:1321	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	2	42	gly	glycoforms	404:413	arg1	protein					396:402	protein glycoforms	396:413	protein glycoforms	396:413	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	0	43	theme	High-throughput	0:14	arg1	N-Glycomics					22:32	High-throughput Serum N-Glycomics	0:32	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.	0:131	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	43	theme	High-throughput	0:14	arg1	Comparison					42:51	Method Comparison	35:51	Method Comparison	35:51	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	43	theme	High-throughput	0:14	arg1	Application					57:67	Application	57:67	Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes	57:130	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	4	44	theme	capillary	881:889	arg1	electrophoresis					895:909	multiplexed capillary gel electrophoresis	869:909	multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling	869:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	0	45	theme	Method	35:40	arg1	N-Glycomics					22:32	High-throughput Serum N-Glycomics	0:32	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.	0:131	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	45	theme	Method	35:40	arg1	Comparison					42:51	Method Comparison	35:51	Method Comparison	35:51	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	4	46	with	chromatography	761:774	arg1	labeling					844:851	2-aminobenzamide labeling	827:851	2-aminobenzamide labeling of the glycans	827:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	labeling					1006:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	xCGE-LIF					954:961	xCGE-LIF	954:961	xCGE-LIF	954:961	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	HILIC-UHPLC-FLD					805:819	HILIC-UHPLC-FLD	805:819	HILIC-UHPLC-FLD	805:819	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	esterification					1146:1159	linkage-specific sialic acid esterification	1117:1159	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	detection					943:951	laser-induced fluorescence detection	916:951	laser-induced fluorescence detection (xCGE-LIF)	916:962	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	46	with	chromatography	761:774	arg1	detection					794:802	fluorescence detection	781:802	fluorescence detection (HILIC-UHPLC-FLD)	781:820	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	47	theme	acid	1001:1004	arg1	labeling					1006:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	3	48	theme	total	530:534	arg1	N-glycosylation					542:556	the total serum N-glycosylation	526:556	the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods	526:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	7	49	theme	non-MS	1708:1713	arg1	methods					1715:1721	the non-MS methods	1704:1721	the non-MS methods	1704:1721	However, the non-MS methods showed superior repeatability over MALDI-TOF-MS and allowed the best structural separation of low-complexity N-glycans.					
30242110	2	50	from	alterations	429:439	arg1	health					444:449	health	444:449	health	444:449	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	2	50	from	alterations	429:439	arg1	disease					455:461	disease	455:461	disease	455:461	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	2	51	gly	glycosylated	351:362	arg1	half					311:314	More than half	301:314	More than half of the proteins in human serum	301:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	10	52	theme	methods	2204:2210	arg1	beneficial					2234:2243	beneficial	2234:2243	beneficial	2234:2243	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	10	52	theme	methods	2204:2210	arg1	combination					2189:2199	the combination	2185:2199	the combination of methods	2185:2210	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	10	53	theme	informed	2302:2309	arg1	choices					2318:2324	informed method choices	2302:2324	informed method choices	2302:2324	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	1	54	theme	serum	259:263	arg1	half-life					265:273	serum half-life	259:273	serum half-life	259:273	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	5	55	theme	serum	1201:1205	arg1	samples					1207:1213	serum samples	1201:1213	serum samples	1201:1213	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	10	56	theme	single	2372:2377	arg1	proteins					2379:2386	single proteins	2372:2386	single proteins	2372:2386	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	2	57	theme	protein	396:402	arg1	glycoforms					404:413	protein glycoforms	396:413	protein glycoforms	396:413	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	6	58	theme	protein	1670:1676	arg1	N-glycosylation					1678:1692	serum protein N-glycosylation	1664:1692	serum protein N-glycosylation	1664:1692	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	10	59	theme	complexity	2410:2419	arg1	proteins					2379:2386	single proteins	2372:2386	single proteins	2372:2386	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	10	59	theme	complexity	2410:2419	arg1	samples					2391:2397	samples	2391:2397	samples of varying complexity	2391:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	6	60	theme	protein	1480:1486	arg1	changes					1504:1510	serum protein N-glycosylation changes	1474:1510	serum protein N-glycosylation changes throughout pregnancy	1474:1531	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	4	61	theme	laser	1036:1040	arg1	MALDI-TOF-MS					1098:1109	MALDI-TOF-MS	1098:1109	MALDI-TOF-MS	1098:1109	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	61	theme	laser	1036:1040	arg1	spectrometry					1084:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1020:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification	1020:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	2	62	theme	proteins	323:330	arg1	half					311:314	More than half	301:314	More than half of the proteins in human serum	301:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	6	63	theme	relative	1637:1644	arg1	quantification					1646:1659	relative quantification	1637:1659	relative quantification	1637:1659	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	4	64	theme	time-of-flight	1064:1077	arg1	MALDI-TOF-MS					1098:1109	MALDI-TOF-MS	1098:1109	MALDI-TOF-MS	1098:1109	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	64	theme	time-of-flight	1064:1077	arg1	spectrometry					1084:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1020:1095	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification	1020:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	65	theme	total	2265:2269	arg1	N-glycosylation					2285:2299	total serum protein N-glycosylation	2265:2299	total serum protein N-glycosylation	2265:2299	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	8	66	theme	highest	1869:1875	arg1	throughput					1877:1886	the highest throughput	1865:1886	the highest throughput	1865:1886	MALDI-TOF-MS achieved the highest throughput and provided compositional information on higher-complexity N-glycans.					
30242110	4	67	theme	fluorescence	781:792	arg1	HILIC-UHPLC-FLD					805:819	HILIC-UHPLC-FLD	805:819	HILIC-UHPLC-FLD	805:819	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	67	theme	fluorescence	781:792	arg1	detection					794:802	fluorescence detection	781:802	fluorescence detection (HILIC-UHPLC-FLD)	781:820	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	68	theme	serum	2271:2275	arg1	N-glycosylation					2285:2299	total serum protein N-glycosylation	2265:2299	total serum protein N-glycosylation	2265:2299	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	2	69	theme	human	335:339	arg1	serum					341:345	human serum	335:345	human serum	335:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	1	70	theme	glycoprotein	222:233	arg1	characteristics					235:249	glycoprotein characteristics	222:249	glycoprotein characteristics such as serum half-life and receptor interaction	222:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	70	theme	glycoprotein	222:233	arg1	half-life					265:273	serum half-life	259:273	serum half-life	259:273	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	70	theme	glycoprotein	222:233	arg1	interaction					288:298	receptor interaction	279:298	receptor interaction	279:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	3	71	theme	released	656:663	arg1	methods					684:690	three high-throughput released N-glycome analysis methods	634:690	three high-throughput released N-glycome analysis methods	634:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	4	72	theme	sialic	1134:1139	arg1	acid					1141:1144	linkage-specific sialic acid	1117:1144	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	73	theme	ultra-high-performance	731:752	arg1	chromatography					761:774	hydrophilic-interaction ultra-high-performance liquid chromatography	707:774	hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans	707:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	6	74	theme	disease	1555:1561	arg1	activity.Overall					1563:1578	RA disease activity.Overall	1552:1578	RA disease activity.Overall	1552:1578	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	5	75	theme	postpartum	1287:1296	arg1	periods					1298:1304	postpartum periods	1287:1304	postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1287:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	7	76	theme	low-complexity	1817:1830	arg1	N-glycans					1832:1840	low-complexity N-glycans	1817:1840	low-complexity N-glycans	1817:1840	However, the non-MS methods showed superior repeatability over MALDI-TOF-MS and allowed the best structural separation of low-complexity N-glycans.					
30242110	6	77	theme	technical	1412:1420	arg1	performance					1422:1432	their technical performance	1406:1432	their technical performance	1406:1432	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	4	78	theme	2-aminobenzamide	827:842	arg1	labeling					844:851	2-aminobenzamide labeling	827:851	2-aminobenzamide labeling of the glycans	827:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	79	theme	N-glycosylation	2285:2299	arg1	analysis					2253:2260	the analysis	2249:2260	the analysis of total serum protein N-glycosylation	2249:2299	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	5	80	theme	time	1248:1251	arg1	points					1253:1258	multiple time points	1239:1258	multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1239:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	2	81	theme	than	306:309	arg1	half					311:314	More than half	301:314	More than half of the proteins in human serum	301:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	2	82	from	half	311:314	arg1	serum					341:345	human serum	335:345	human serum	335:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	9	83	theme	sialylation	2026:2036	arg1	differences					2038:2048	the linkage-specific sialylation differences	2005:2048	the linkage-specific sialylation differences within pregnancy and RA	2005:2072	Consequentially, MALDI-TOF-MS could establish the linkage-specific sialylation differences within pregnancy and RA, whereas HILIC-UHPLC-FLD and xCGE-LIF demonstrated differences in α1,3- and α1,6-branch galactosylation.					
30242110	0	84	theme	Study	72:76	arg1	Arthritis					89:97	Study Rheumatoid Arthritis	72:97	Study Rheumatoid Arthritis	72:97	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	3	85	theme	analysis	675:682	arg1	methods					684:690	three high-throughput released N-glycome analysis methods	634:690	three high-throughput released N-glycome analysis methods	634:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	1	86	theme	important	168:176	arg1	N-Glycosylation					133:147	N-Glycosylation	133:147	N-Glycosylation	133:147	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	1	86	theme	important	168:176	arg1	modification					186:197	a fundamentally important protein modification	152:197	a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction	152:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	6	87	theme	analytical	1384:1393	arg1	methods					1395:1401	the analytical methods	1380:1401	the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy,	1380:1532	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	88	from	methods	1395:1401	arg1	performance					1422:1432	their technical performance	1406:1432	their technical performance	1406:1432	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	88	from	methods	1395:1401	arg1	ability					1454:1460	their ability to describe serum protein N-glycosylation changes throughout pregnancy	1448:1531	their ability to describe serum protein N-glycosylation changes throughout pregnancy	1448:1531	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	5	89	theme	patients	1327:1334	arg1	pregnancies					1271:1281	the pregnancies	1267:1281	the pregnancies	1267:1281	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	5	89	theme	patients	1327:1334	arg1	periods					1298:1304	postpartum periods	1287:1304	postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1287:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	1	90	with	modification	186:197	arg1	impact					212:217	a major impact	204:217	a major impact on glycoprotein characteristics such as serum half-life and receptor interaction	204:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
30242110	10	91	theme	glycosylation	2346:2358	arg1	analysis					2360:2367	the glycosylation analysis	2342:2367	the glycosylation analysis of single proteins or samples of varying complexity	2342:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	0	92	theme	Pregnancy-associated	103:122	arg1	Changes					124:130	Pregnancy-associated Changes	103:130	Pregnancy-associated Changes	103:130	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	5	93	theme	rheumatoid	1341:1350	arg1	RA					1363:1364	RA	1363:1364	RA	1363:1364	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	5	93	theme	rheumatoid	1341:1350	arg1	arthritis					1352:1360	rheumatoid arthritis	1341:1360	rheumatoid arthritis (RA)	1341:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	2	94	from	serum	341:345	arg1	half					311:314	More than half	301:314	More than half of the proteins in human serum	301:345	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	4	95	theme	gel	891:893	arg1	electrophoresis					895:909	multiplexed capillary gel electrophoresis	869:909	multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling	869:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	5	96	theme	same	1187:1190	arg1	panel					1192:1196	the same panel	1183:1196	the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1183:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	0	97	theme	Serum	16:20	arg1	N-Glycomics					22:32	High-throughput Serum N-Glycomics	0:32	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.	0:131	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	97	theme	Serum	16:20	arg1	Comparison					42:51	Method Comparison	35:51	Method Comparison	35:51	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	0	97	theme	Serum	16:20	arg1	Application					57:67	Application	57:67	Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes	57:130	High-throughput Serum N-Glycomics: Method Comparison and Application to Study Rheumatoid Arthritis and Pregnancy-associated Changes.					
30242110	5	98	theme	women	1317:1321	arg1	pregnancies					1271:1281	the pregnancies	1267:1281	the pregnancies	1267:1281	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	5	98	theme	women	1317:1321	arg1	periods					1298:1304	postpartum periods	1287:1304	postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1287:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	3	99	dep	N-glycosylation	542:556	arg1	evaluate					594:601	evaluate	594:601	evaluate	594:601	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	3	99	dep	N-glycosylation	542:556	arg1	compare					607:613	compare	607:613	compare the performance of three high-throughput released N-glycome analysis methods	607:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	4	100	theme	glycans	860:866	arg1	labeling					844:851	2-aminobenzamide labeling	827:851	2-aminobenzamide labeling of the glycans	827:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	2	101	theme	glycoforms	404:413	arg1	abundances					382:391	the relative abundances	369:391	the relative abundances of protein glycoforms	369:413	More than half of the proteins in human serum are glycosylated, and the relative abundances of protein glycoforms often reflect alterations in health and disease.					
30242110	4	102	theme	multiplexed	869:879	arg1	electrophoresis					895:909	multiplexed capillary gel electrophoresis	869:909	multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling	869:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	10	103	gly	glycosylation	2346:2358	arg1	proteins					2379:2386	single proteins	2372:2386	single proteins	2372:2386	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	10	103	gly	glycosylation	2346:2358	arg1	samples					2391:2397	samples	2391:2397	samples of varying complexity	2391:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	4	104	with	spectrometry	1084:1095	arg1	labeling					844:851	2-aminobenzamide labeling	827:851	2-aminobenzamide labeling of the glycans	827:866	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	labeling					1006:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	xCGE-LIF					954:961	xCGE-LIF	954:961	xCGE-LIF	954:961	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	HILIC-UHPLC-FLD					805:819	HILIC-UHPLC-FLD	805:819	HILIC-UHPLC-FLD	805:819	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	esterification					1146:1159	linkage-specific sialic acid esterification	1117:1159	linkage-specific sialic acid esterification	1117:1159	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	detection					943:951	laser-induced fluorescence detection	916:951	laser-induced fluorescence detection (xCGE-LIF)	916:962	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	104	with	spectrometry	1084:1095	arg1	detection					794:802	fluorescence detection	781:802	fluorescence detection (HILIC-UHPLC-FLD)	781:820	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	9	105	theme	α1,3-	2140:2144	arg1	galactosylation					2162:2176	α1,3- and α1,6-branch galactosylation	2140:2176	α1,3- and α1,6-branch galactosylation	2140:2176	Consequentially, MALDI-TOF-MS could establish the linkage-specific sialylation differences within pregnancy and RA, whereas HILIC-UHPLC-FLD and xCGE-LIF demonstrated differences in α1,3- and α1,6-branch galactosylation.					
30242110	7	106	theme	superior	1730:1737	arg1	repeatability					1739:1751	superior repeatability	1730:1751	superior repeatability	1730:1751	However, the non-MS methods showed superior repeatability over MALDI-TOF-MS and allowed the best structural separation of low-complexity N-glycans.					
30242110	9	107	theme	α1,6-branch	2150:2160	arg1	galactosylation					2162:2176	α1,3- and α1,6-branch galactosylation	2140:2176	α1,3- and α1,6-branch galactosylation	2140:2176	Consequentially, MALDI-TOF-MS could establish the linkage-specific sialylation differences within pregnancy and RA, whereas HILIC-UHPLC-FLD and xCGE-LIF demonstrated differences in α1,3- and α1,6-branch galactosylation.					
30242110	4	108	theme	8-aminopyrene-1,3,6-trisulfonic	969:999	arg1	labeling					1006:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	8-aminopyrene-1,3,6-trisulfonic acid labeling	969:1013	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	3	109	theme	serum	536:540	arg1	N-glycosylation					542:556	the total serum N-glycosylation	526:556	the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods	526:690	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	6	110	from	quantification	1646:1659	arg1	similar					1606:1612	similar	1606:1612	similar	1606:1612	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	110	from	quantification	1646:1659	arg1	methods					1585:1591	the methods	1581:1591	the methods	1581:1591	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	10	111	theme	proteins	2379:2386	arg1	analysis					2360:2367	the glycosylation analysis	2342:2367	the glycosylation analysis of single proteins or samples of varying complexity	2342:2419	While the combination of methods proved to be the most beneficial for the analysis of total serum protein N-glycosylation, informed method choices can be made for the glycosylation analysis of single proteins or samples of varying complexity.					
30242110	4	112	theme	fluorescence	930:941	arg1	xCGE-LIF					954:961	xCGE-LIF	954:961	xCGE-LIF	954:961	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	4	112	theme	fluorescence	930:941	arg1	detection					943:951	laser-induced fluorescence detection	916:951	laser-induced fluorescence detection (xCGE-LIF)	916:962	Included were hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) with 2-aminobenzamide labeling of the glycans, multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) with 8-aminopyrene-1,3,6-trisulfonic acid labeling, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with linkage-specific sialic acid esterification.					
30242110	6	113	theme	N-glycosylation	1678:1692	arg1	detection					1623:1631	their detection	1617:1631	their detection	1617:1631	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	6	113	theme	N-glycosylation	1678:1692	arg1	quantification					1646:1659	relative quantification	1637:1659	relative quantification	1637:1659	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	5	114	theme	samples	1207:1213	arg1	panel					1192:1196	the same panel	1183:1196	the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA)	1183:1365	All methods assessed the same panel of serum samples, which were obtained at multiple time points during the pregnancies and postpartum periods of healthy women and patients with rheumatoid arthritis (RA).					
30242110	8	115	theme	higher-complexity	1930:1946	arg1	N-glycans					1948:1956	higher-complexity N-glycans	1930:1956	higher-complexity N-glycans	1930:1956	MALDI-TOF-MS achieved the highest throughput and provided compositional information on higher-complexity N-glycans.					
30242110	3	116	theme	high-throughput	563:577	arg1	manner.Here					579:589	a high-throughput manner.Here	561:589	a high-throughput manner.Here	561:589	Several analytical methods are currently capable of analyzing the total serum N-glycosylation in a high-throughput manner.Here we evaluate and compare the performance of three high-throughput released N-glycome analysis methods.					
30242110	6	117	theme	serum	1664:1668	arg1	N-glycosylation					1678:1692	serum protein N-glycosylation	1664:1692	serum protein N-glycosylation	1664:1692	We compared the analytical methods on their technical performance as well as on their ability to describe serum protein N-glycosylation changes throughout pregnancy, with RA, and with RA disease activity.Overall, the methods proved to be similar in their detection and relative quantification of serum protein N-glycosylation.					
30242110	1	118	theme	receptor	279:286	arg1	interaction					288:298	receptor interaction	279:298	receptor interaction	279:298	N-Glycosylation is a fundamentally important protein modification with a major impact on glycoprotein characteristics such as serum half-life and receptor interaction.					
29930140	8	0	from	prognosis	1860:1868	arg1	patients					1873:1880	patients	1873:1880	patients with type 2 diabetes	1873:1901	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	8	1	dep	CONCLUSIONS	1761:1771	arg1	useful					1832:1837	useful	1832:1837	useful	1832:1837	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	8	1	dep	CONCLUSIONS	1761:1771	arg1	profile					1792:1798	The urinary glycan profile	1773:1798	The urinary glycan profile identified in this study	1773:1823	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	7	2	theme	reclassification	1558:1573	arg1	improvement					1575:1585	net reclassification improvement	1554:1585	net reclassification improvement	1554:1585	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	9	3	theme	Additional	1904:1913	arg1	investigation					1915:1927	Additional investigation	1904:1927	Additional investigation of glycosylation changes and urinary glycan excretion in DKD	1904:1988	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	1	4	with	association	302:312	arg1	prognosis					355:363	the renal prognosis	345:363	the renal prognosis in diabetic kidney disease (DKD)	345:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	7	5	dep	287	1755:1757	arg1	to					1752:1753	to	1752:1753	to	1752:1753	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	9	6	theme	changes	1946:1952	arg1	investigation					1915:1927	Additional investigation	1904:1927	Additional investigation of glycosylation changes and urinary glycan excretion in DKD	1904:1988	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	6	7	theme	Hazard	1089:1094	arg1	ratios					1096:1101	Hazard ratios	1089:1101	Hazard ratios for these lectins	1089:1119	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	9	8	theme	urinary	1958:1964	arg1	excretion					1973:1981	urinary glycan excretion	1958:1981	urinary glycan excretion	1958:1981	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	0	9	theme	Glycan	110:115	arg1	Profiling					117:125	Glycan Profiling	110:125	Glycan Profiling	110:125	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	9	10	theme	excretion	1973:1981	arg1	investigation					1915:1927	Additional investigation	1904:1927	Additional investigation of glycosylation changes and urinary glycan excretion in DKD	1904:1988	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	6	11	theme	%	1219:1219	arg1	SNA					1167:1169	SNA	1167:1169	SNA	1167:1169	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	11	theme	%	1219:1219	arg1	CI					1221:1222	95% CI 1.14-1.76	1217:1232	95% CI 1.14-1.76	1217:1232	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	1	12	from	prognosis	355:363	arg1	DKD					393:395	DKD	393:395	DKD	393:395	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	1	12	from	prognosis	355:363	arg1	disease					384:390	diabetic kidney disease	368:390	diabetic kidney disease (DKD)	368:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	1	13	theme	quantifying	204:214	arg1	glycans					216:222	quantifying glycans	204:222	quantifying glycans with complex structures	204:246	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	0	14	from	Prognosis	64:72	arg1	Patients					77:84	Patients	77:84	Patients With Type 2 Diabetes	77:105	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	5	15	theme	urinary	992:998	arg1	glycans					1000:1006	these urinary glycans	986:1006	these urinary glycans	986:1006	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	3	16	theme	end	569:571	arg1	point					573:577	The end point	565:577	The end point	565:577	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	3	16	theme	end	569:571	arg1	decrease					585:592	a decrease	583:592	a decrease of estimated glomerular filtration rate (eGFR)	583:639	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	2	17	with	patients	434:441	arg1	diabetes					455:462	type 2 diabetes	448:462	type 2 diabetes	448:462	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	7	18	contain	containing	1475:1484	arg1	model					1469:1473	a model	1467:1473	a model containing known indicators of progression	1467:1516	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	7	18	contain	containing	1475:1484	arg2	indicators					1492:1501	known indicators	1486:1501	known indicators of progression	1486:1516	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	2	19	theme	different	540:548	arg1	specificities					550:562	different specificities	540:562	different specificities	540:562	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	0	20	theme	Cohort	156:161	arg1	Study					163:167	a Multicenter Prospective Cohort Study	130:167	a Multicenter Prospective Cohort Study	130:167	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	3	21	theme	estimated	597:605	arg1	rate					629:632	estimated glomerular filtration rate	597:632	estimated glomerular filtration rate (eGFR)	597:639	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	3	21	theme	estimated	597:605	arg1	eGFR					635:638	eGFR	635:638	eGFR	635:638	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	7	22	theme	known	1486:1490	arg1	indicators					1492:1501	known indicators	1486:1501	known indicators of progression	1486:1516	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	5	23	theme	baseline	1058:1065	arg1	albuminuria					1067:1077	baseline albuminuria	1058:1077	baseline albuminuria	1058:1077	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	9	24	from	investigation	1915:1927	arg1	DKD					1986:1988	DKD	1986:1988	DKD	1986:1988	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	3	25	theme	end-stage	679:687	arg1	disease					695:701	end-stage renal disease	679:701	end-stage renal disease	679:701	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	5	26	theme	glycans	853:859	arg1	levels					843:848	urinary levels	835:848	urinary levels of glycans binding to six lectins	835:882	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	4	27	theme	median	721:726	arg1	follow-up					728:736	a median follow-up	719:736	a median follow-up of 4.0 years	719:749	RESULTS During a median follow-up of 4.0 years, 63 patients reached the end point.					
29930140	0	28	theme	Type	91:94	arg1	Diabetes					98:105	Type 2 Diabetes	91:105	Type 2 Diabetes	91:105	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	7	29	theme	glycan	1449:1454	arg1	indexes					1456:1462	these glycan indexes	1443:1462	these glycan indexes	1443:1462	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	2	30	theme	urinary	490:496	arg1	signals					505:511	the baseline urinary glycan signals	477:511	the baseline urinary glycan signals binding to 45 lectins with different specificities	477:562	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	1	31	theme	diabetic	368:375	arg1	DKD					393:395	DKD	393:395	DKD	393:395	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	1	31	theme	diabetic	368:375	arg1	disease					384:390	diabetic kidney disease	368:390	diabetic kidney disease (DKD)	368:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	7	32	theme	Akaike	1704:1709	arg1	criterion					1723:1731	the Akaike information criterion	1700:1731	the Akaike information criterion	1700:1731	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	8	33	theme	glycan	1785:1790	arg1	useful					1832:1837	useful	1832:1837	useful	1832:1837	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	8	33	theme	glycan	1785:1790	arg1	profile					1792:1798	The urinary glycan profile	1773:1798	The urinary glycan profile identified in this study	1773:1823	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	0	34	theme	Novel	18:22	arg1	Biomarkers					32:41	Novel Urinary Biomarkers	18:41	Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study	18:167	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	2	35	theme	RESEARCH	399:406	arg1	DESIGN					408:413	RESEARCH DESIGN	399:413	RESEARCH DESIGN	399:413	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	0	36	theme	Biomarkers	32:41	arg1	Identification					0:13	Identification	0:13	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.	0:184	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	2	37	theme	type	448:451	arg1	diabetes					455:462	type 2 diabetes	448:462	type 2 diabetes	448:462	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	3	38	theme	filtration	618:627	arg1	rate					629:632	estimated glomerular filtration rate	597:632	estimated glomerular filtration rate (eGFR)	597:639	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	3	38	theme	filtration	618:627	arg1	eGFR					635:638	eGFR	635:638	eGFR	635:638	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	4	39	theme	end	776:778	arg1	point					780:784	the end point	772:784	the end point	772:784	RESULTS During a median follow-up of 4.0 years, 63 patients reached the end point.					
29930140	1	40	theme	profiles	331:338	arg1	association					302:312	the association	298:312	the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD)	298:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	8	41	theme	type	1887:1890	arg1	diabetes					1894:1901	type 2 diabetes	1887:1901	type 2 diabetes	1887:1901	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	4	42	theme	years	745:749	arg1	follow-up					728:736	a median follow-up	719:736	a median follow-up of 4.0 years	719:749	RESULTS During a median follow-up of 4.0 years, 63 patients reached the end point.					
29930140	7	43	theme	discrimination	1638:1651	arg1	improvement					1653:1663	relative integrated discrimination improvement	1618:1663	relative integrated discrimination improvement	1618:1663	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	5	44	theme	known	952:956	arg1	indicators					958:967	known indicators	952:967	known indicators of DKD	952:974	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	1	45	theme	complex	229:235	arg1	structures					237:246	complex structures	229:246	complex structures	229:246	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	0	46	dep	Identification	0:13	arg1	Study					177:181	U-CARE Study 1	170:183	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.	0:184	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	1	47	with	glycans	216:222	arg1	structures					237:246	complex structures	229:246	complex structures	229:246	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	0	48	theme	U-CARE	170:175	arg1	Study					177:181	U-CARE Study 1	170:183	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.	0:184	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	7	49	theme	net	1554:1556	arg1	improvement					1575:1585	net reclassification improvement	1554:1585	net reclassification improvement	1554:1585	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	6	50	theme	glycan	1184:1189	arg1	Siaα2-6Gal/GalNAc					1191:1207	glycan Siaα2-6Gal/GalNAc	1184:1207	glycan Siaα2-6Gal/GalNAc	1184:1207	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	0	51	with	Patients	77:84	arg1	Diabetes					98:105	Type 2 Diabetes	91:105	Type 2 Diabetes	91:105	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	7	52	theme	outcome	1545:1551	arg1	prediction					1527:1536	prediction	1527:1536	prediction of the outcome	1527:1551	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	3	53	theme	renal	689:693	arg1	disease					695:701	end-stage renal disease	679:701	end-stage renal disease	679:701	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	5	54	theme	hazards	804:810	arg1	analysis					812:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	3	55	from	dialysis	666:673	arg1	%					647:647	≥30%	644:647	≥30% from baseline or dialysis for end-stage renal disease	644:701	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	9	56	theme	glycosylation	1932:1944	arg1	changes					1946:1952	glycosylation changes	1932:1952	glycosylation changes	1932:1952	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	9	57	theme	glycan	1966:1971	arg1	excretion					1973:1981	urinary glycan excretion	1958:1981	urinary glycan excretion	1958:1981	Additional investigation of glycosylation changes and urinary glycan excretion in DKD is needed.					
29930140	5	58	theme	Cox	787:789	arg1	analysis					812:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	6	59	dep	were	1121:1124	arg1	ACA					1399:1401	ACA	1399:1401	ACA	1399:1401	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	CI					1221:1222	95% CI 1.14-1.76	1217:1232	95% CI 1.14-1.76	1217:1232	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	1.04-1.67					1424:1432	1.04-1.67	1424:1432	1.04-1.67	1424:1432	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	SNA					1167:1169	SNA	1167:1169	SNA	1167:1169	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	1.02-1.67					1383:1391	1.02-1.67	1383:1391	1.02-1.67	1383:1391	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	0.64-0.997					1304:1313	0.64-0.997	1304:1313	0.64-0.997	1304:1313	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	RCA120					1236:1241	RCA120	1236:1241	RCA120	1236:1241	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	1.01-1.64					1264:1272	1.01-1.64	1264:1272	1.01-1.64	1264:1272	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	Galβ4GlcNAc					1244:1254	Galβ4GlcNAc	1244:1254	Galβ4GlcNAc	1244:1254	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	SD					1130:1131	+1 SD	1127:1131	+1 SD for the glycan index	1127:1152	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	59	dep	were	1121:1124	arg1	1.02-1.64					1342:1350	1.02-1.64	1342:1350	1.02-1.64	1342:1350	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	3	60	from	baseline	654:661	arg1	%					647:647	≥30%	644:647	≥30% from baseline or dialysis for end-stage renal disease	644:701	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	0	61	theme	Prospective	144:154	arg1	Study					163:167	a Multicenter Prospective Cohort Study	130:167	a Multicenter Prospective Cohort Study	130:167	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	6	62	theme	glycan	1141:1146	arg1	index					1148:1152	the glycan index	1137:1152	the glycan index	1137:1152	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	63	theme	1.42	1211:1214	arg1	SNA					1167:1169	SNA	1167:1169	SNA	1167:1169	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	63	theme	1.42	1211:1214	arg1	CI					1221:1222	95% CI 1.14-1.76	1217:1232	95% CI 1.14-1.76	1217:1232	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	7	64	theme	progression	1506:1516	arg1	indicators					1492:1501	known indicators	1486:1501	known indicators of progression	1486:1516	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	5	65	theme	urinary	835:841	arg1	levels					843:848	urinary levels	835:848	urinary levels of glycans binding to six lectins	835:882	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	6	66	dep	RCA120	1236:1241	arg1	DBA					1276:1278	DBA	1276:1278	DBA (GalNAcα3GalNAc)	1276:1295	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	66	dep	RCA120	1236:1241	arg1	Jacalin					1354:1360	Jacalin	1354:1360	Jacalin (Galβ3GalNAc)	1354:1374	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	66	dep	RCA120	1236:1241	arg1	GalNAcα3GalNAc					1281:1294	GalNAcα3GalNAc	1281:1294	GalNAcα3GalNAc	1281:1294	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	66	dep	RCA120	1236:1241	arg1	Galβ3GalNAc					1363:1373	Galβ3GalNAc	1363:1373	Galβ3GalNAc	1363:1373	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	66	dep	RCA120	1236:1241	arg1	ABA					1317:1319	ABA	1317:1319	ABA (Galβ3GalNAc)	1317:1333	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	6	66	dep	RCA120	1236:1241	arg1	Galβ3GalNAc					1322:1332	Galβ3GalNAc	1322:1332	Galβ3GalNAc	1322:1332	Hazard ratios for these lectins were (+1 SD for the glycan index) as follows: SNA (recognizing glycan Siaα2-6Gal/GalNAc), 1.42 (95% CI 1.14-1.76); RCA120 (Galβ4GlcNAc), 1.28 (1.01-1.64); DBA (GalNAcα3GalNAc), 0.80 (0.64-0.997); ABA (Galβ3GalNAc), 1.29 (1.02-1.64); Jacalin (Galβ3GalNAc), 1.30 (1.02-1.67); and ACA (Galβ3GalNAc), 1.32 (1.04-1.67).					
29930140	1	67	theme	renal	349:353	arg1	prognosis					355:363	the renal prognosis	345:363	the renal prognosis in diabetic kidney disease (DKD)	345:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	7	68	theme	information	1711:1721	arg1	criterion					1723:1731	the Akaike information criterion	1700:1731	the Akaike information criterion	1700:1731	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	2	69	theme	glycan	498:503	arg1	signals					505:511	the baseline urinary glycan signals	477:511	the baseline urinary glycan signals binding to 45 lectins with different specificities	477:562	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	8	70	theme	urinary	1777:1783	arg1	useful					1832:1837	useful	1832:1837	useful	1832:1837	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	8	70	theme	urinary	1777:1783	arg1	profile					1792:1798	The urinary glycan profile	1773:1798	The urinary glycan profile identified in this study	1773:1823	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	8	71	with	patients	1873:1880	arg1	diabetes					1894:1901	type 2 diabetes	1887:1901	type 2 diabetes	1887:1901	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	2	72	theme	baseline	481:488	arg1	signals					505:511	the baseline urinary glycan signals	477:511	the baseline urinary glycan signals binding to 45 lectins with different specificities	477:562	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	1	73	theme	kidney	377:382	arg1	DKD					393:395	DKD	393:395	DKD	393:395	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	1	73	theme	kidney	377:382	arg1	disease					384:390	diabetic kidney disease	368:390	diabetic kidney disease (DKD)	368:396	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	8	74	theme	renal	1854:1858	arg1	prognosis					1860:1868	renal prognosis	1854:1868	renal prognosis in patients with type 2 diabetes	1854:1901	CONCLUSIONS The urinary glycan profile identified in this study may be useful for predicting renal prognosis in patients with type 2 diabetes.					
29930140	0	75	theme	Urinary	24:30	arg1	Biomarkers					32:41	Novel Urinary Biomarkers	18:41	Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study	18:167	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	3	76	theme	rate	629:632	arg1	point					573:577	The end point	565:577	The end point	565:577	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	3	76	theme	rate	629:632	arg1	decrease					585:592	a decrease	583:592	a decrease of estimated glomerular filtration rate (eGFR)	583:639	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	5	77	theme	DKD	972:974	arg1	indicators					958:967	known indicators	952:967	known indicators of DKD	952:974	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29930140	3	78	theme	glomerular	607:616	arg1	rate					629:632	estimated glomerular filtration rate	597:632	estimated glomerular filtration rate (eGFR)	597:639	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	3	78	theme	glomerular	607:616	arg1	eGFR					635:638	eGFR	635:638	eGFR	635:638	The end point was a decrease of estimated glomerular filtration rate (eGFR) by ≥30% from baseline or dialysis for end-stage renal disease.					
29930140	0	79	theme	Renal	58:62	arg1	Prognosis					64:72	Renal Prognosis	58:72	Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling	58:125	Identification of Novel Urinary Biomarkers for Predicting Renal Prognosis in Patients With Type 2 Diabetes by Glycan Profiling in a Multicenter Prospective Cohort Study: U-CARE Study 1.					
29930140	7	80	theme	relative	1618:1625	arg1	improvement					1653:1663	relative integrated discrimination improvement	1618:1663	relative integrated discrimination improvement	1618:1663	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	2	81	dep	DESIGN	408:413	arg1	patients					434:441	675 patients	430:441	675 patients with type 2 diabetes	430:462	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	1	82	theme	glycosylation	317:329	arg1	profiles					331:338	glycosylation profiles	317:338	glycosylation profiles	317:338	OBJECTIVE Because quantifying glycans with complex structures is technically challenging, little is known about the association of glycosylation profiles with the renal prognosis in diabetic kidney disease (DKD).					
29930140	7	83	theme	integrated	1627:1636	arg1	improvement					1653:1663	relative integrated discrimination improvement	1618:1663	relative integrated discrimination improvement	1618:1663	Adding these glycan indexes to a model containing known indicators of progression improved prediction of the outcome (net reclassification improvement increased by 0.51 [0.22-0.80], relative integrated discrimination improvement increased by 0.18 [0.01-0.35], and the Akaike information criterion decreased from 296 to 287).					
29930140	2	84	with	lectins	527:533	arg1	specificities					550:562	different specificities	540:562	different specificities	540:562	RESEARCH DESIGN AND METHODS In 675 patients with type 2 diabetes, we assessed the baseline urinary glycan signals binding to 45 lectins with different specificities.					
29930140	5	85	theme	proportional	791:802	arg1	analysis					812:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis	787:819	Cox proportional hazards analysis revealed that urinary levels of glycans binding to six lectins were significantly associated with the outcome after adjustment for known indicators of DKD, although these urinary glycans, except that for DBA, were highly correlated with baseline albuminuria and eGFR.					
29615495	0	0	theme	Mass	92:95	arg1	Spectra					97:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	8	1	theme	peak	1457:1460	arg1	picking					1462:1468	peak picking	1457:1468	peak picking	1457:1468	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	9	2	theme	spectrum	1606:1613	arg1	analysis					1615:1622	spectrum analysis	1606:1622	spectrum analysis	1606:1622	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	3	3	theme	GAG	498:500	arg1	saccharides					502:512	GAG saccharides	498:512	GAG saccharides	498:512	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	8	4	theme	ion	1409:1411	arg1	composition					1413:1423	a given product ion composition	1393:1423	a given product ion composition	1393:1423	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	0	5	theme	Tandem	85:90	arg1	Spectra					97:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	1	6	attach	linked	143:148	arg1	proteoglycans					153:165	proteoglycans	153:165	proteoglycans (PGs)	153:171	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	1	6	attach	linked	143:148	arg2	Glycosaminoglycans					106:123	Glycosaminoglycans	106:123	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs)	106:171	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	1	6	attach	linked	143:148	arg1	PGs					168:170	PGs	168:170	PGs	168:170	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	1	6	attach	linked	143:148	arg2	GAGs					126:129	GAGs	126:129	GAGs	126:129	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	2	7	theme	structural	355:364	arg1	support					366:372	structural support	355:372	structural support	355:372	GAG length and sulfation patterns impact disease etiology, cellular signaling, and structural support for cells.					
29615495	10	8	theme	averagine-based	1809:1823	arg1	methods					1825:1831	averagine-based methods	1809:1831	averagine-based methods	1809:1831	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	5	9	theme	building	967:974	arg1	block					976:980	a single average building block	950:980	a single average building block	950:980	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	6	10	from	addition	1057:1064	arg1	necessary					1073:1081	necessary	1073:1081	necessary	1073:1081	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	6	11	theme	isotope	1105:1111	arg1	patterns					1113:1120	product ion isotope patterns	1093:1120	product ion isotope patterns	1093:1120	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	5	12	theme	GAG	917:919	arg1	spectra					933:939	GAG tandem mass spectra	917:939	GAG tandem mass spectra	917:939	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	6	13	theme	mass	1146:1149	arg1	spectra					1151:1157	the tandem mass spectra	1135:1157	the tandem mass spectra of GAG saccharides	1135:1176	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	6	14	theme	saccharides	1166:1176	arg1	spectra					1151:1157	the tandem mass spectra	1135:1157	the tandem mass spectra of GAG saccharides	1135:1176	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	2	15	theme	sulfation	287:295	arg1	patterns					297:304	GAG length and sulfation patterns	272:304	patterns	297:304	GAG length and sulfation patterns impact disease etiology, cellular signaling, and structural support for cells.					
29615495	4	16	theme	peaks	692:696	arg1	identification					661:674	the identification	657:674	the identification of monoisotopic peaks and charge states	657:714	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	6	17	theme	ion	1101:1103	arg1	patterns					1113:1120	product ion isotope patterns	1093:1120	product ion isotope patterns	1093:1120	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	9	18	theme	precursor	1634:1642	arg1	composition					1644:1654	precursor composition	1634:1654	precursor composition information	1634:1666	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	7	19	theme	finding	1266:1272	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	19	theme	finding	1266:1272	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	4	20	theme	average	764:770	arg1	block					781:785	the average building block	760:785	the average building block of the compound class being analyzed	760:822	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	3	21	theme	mass	442:445	arg1	MS2					461:463	MS2	461:463	MS2	461:463	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	3	21	theme	mass	442:445	arg1	spectrometry					447:458	tandem mass spectrometry	435:458	tandem mass spectrometry (MS2)	435:464	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	9	22	theme	composition	1644:1654	arg1	information					1656:1666	precursor composition information	1634:1666	precursor composition information	1634:1666	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	3	23	theme	manual	524:529	arg1	interpretation					531:544	manual interpretation	524:544	manual interpretation of tandem mass spectra	524:567	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	3	24	theme	spectrometry	447:458	arg1	usefulness					421:430	the usefulness	417:430	the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides	417:512	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	8	25	theme	existing	1518:1525	arg1	methods					1527:1533	the existing methods	1514:1533	the existing methods previously mentioned	1514:1554	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	8	26	theme	experimental	1353:1364	arg1	peaks					1375:1379	experimental isotopic peaks	1353:1379	experimental isotopic peaks	1353:1379	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	3	27	theme	spectra	561:567	arg1	interpretation					531:544	manual interpretation	524:544	manual interpretation of tandem mass spectra	524:567	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	10	28	theme	isotope	1736:1742	arg1	step					1800:1803	a subsequent step	1787:1803	a subsequent step for averagine-based methods	1787:1831	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	10	28	theme	isotope	1736:1742	arg1	annotation					1756:1765	peak isotope composition annotation	1731:1765	peak isotope composition annotation	1731:1765	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	10	29	theme	subsequent	1789:1798	arg1	step					1800:1803	a subsequent step	1787:1803	a subsequent step for averagine-based methods	1787:1831	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	10	29	theme	subsequent	1789:1798	arg1	annotation					1756:1765	peak isotope composition annotation	1731:1765	peak isotope composition annotation	1731:1765	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	8	30	dep	define	1320:1325	arg1	terms					1481:1485	terms	1481:1485	terms more accurately describing the existing methods previously mentioned	1481:1554	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	8	30	dep	define	1320:1325	arg1	which					1471:1475	which	1471:1475	which	1471:1475	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	5	31	theme	tandem	921:926	arg1	spectra					933:939	GAG tandem mass spectra	917:939	GAG tandem mass spectra	917:939	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	3	32	theme	tandem	435:440	arg1	MS2					461:463	MS2	461:463	MS2	461:463	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	3	32	theme	tandem	435:440	arg1	spectrometry					447:458	tandem mass spectrometry	435:458	tandem mass spectrometry (MS2)	435:464	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	8	33	theme	product	1401:1407	arg1	composition					1413:1423	a given product ion composition	1393:1423	a given product ion composition	1393:1423	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	2	34	theme	disease	313:319	arg1	etiology					321:328	disease etiology	313:328	disease etiology	313:328	GAG length and sulfation patterns impact disease etiology, cellular signaling, and structural support for cells.					
29615495	8	35	theme	isotopic	1366:1373	arg1	peaks					1375:1379	experimental isotopic peaks	1353:1379	experimental isotopic peaks	1353:1379	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	1	36	theme	repeating	194:202	arg1	units					217:221	repeating disaccharide units	194:221	repeating disaccharide units	194:221	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	0	37	theme	Peak	13:16	arg1	Finding					18:24	Peak Finding	13:24	Peak Finding	13:24	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	6	38	theme	tandem	1139:1144	arg1	spectra					1151:1157	the tandem mass spectra	1135:1157	the tandem mass spectra of GAG saccharides	1135:1176	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	5	39	theme	units	1047:1051	arg1	sulfation					1017:1025	the variable sulfation	1004:1025	the variable sulfation of GAG disaccharide units	1004:1051	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	3	40	theme	mass	556:559	arg1	spectra					561:567	tandem mass spectra	549:567	tandem mass spectra	549:567	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	10	41	theme	composition	1744:1754	arg1	step					1800:1803	a subsequent step	1787:1803	a subsequent step for averagine-based methods	1787:1831	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	10	41	theme	composition	1744:1754	arg1	annotation					1756:1765	peak isotope composition annotation	1731:1765	peak isotope composition annotation	1731:1765	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	1	42	theme	disaccharide	204:215	arg1	units					217:221	repeating disaccharide units	194:221	repeating disaccharide units	194:221	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	0	43	theme	Elemental	30:38	arg1	Composition					40:50	Elemental Composition	30:50	Elemental Composition	30:50	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	6	44	theme	product	1093:1099	arg1	patterns					1113:1120	product ion isotope patterns	1093:1120	product ion isotope patterns	1093:1120	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	9	45	theme	theoretical	1684:1694	arg1	fragments					1696:1704	all theoretical fragments	1680:1704	all theoretical fragments	1680:1704	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	7	46	theme	mass	1247:1250	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	46	theme	mass	1247:1250	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	4	47	theme	class	803:807	arg1	averagine					746:754	averagine	746:754	averagine	746:754	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	4	47	theme	class	803:807	arg1	block					781:785	the average building block	760:785	the average building block of the compound class being analyzed	760:822	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	4	48	theme	proteomics	638:647	arg1	domain					649:654	the proteomics domain	634:654	the proteomics domain	634:654	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	9	49	dep	targeted	1572:1579	arg1	brute					1582:1586	brute	1582:1586	brute	1582:1586	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	9	50	theme	force	1588:1592	arg1	approach					1594:1601	a targeted, brute force approach	1570:1601	a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments	1570:1704	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	9	50	theme	force	1588:1592	arg1	GAGfinder					1557:1565	GAGfinder	1557:1565	GAGfinder	1557:1565	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	3	51	theme	computational	591:603	arg1	methods					605:611	computational methods	591:611	computational methods	591:611	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	7	52	theme	spectrum	1252:1259	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	52	theme	spectrum	1252:1259	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	53	theme	first	1234:1238	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	53	theme	first	1234:1238	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	9	54	theme	targeted	1572:1579	arg1	approach					1594:1601	a targeted, brute force approach	1570:1601	a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments	1570:1704	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	9	54	theme	targeted	1572:1579	arg1	GAGfinder					1557:1565	GAGfinder	1557:1565	GAGfinder	1557:1565	GAGfinder is a targeted, brute force approach to spectrum analysis that uses precursor composition information to generate all theoretical fragments.					
29615495	8	55	theme	peak	1327:1330	arg1	finding					1332:1338	peak finding	1327:1338	peak finding	1327:1338	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	4	56	theme	states	709:714	arg1	identification					661:674	the identification	657:674	the identification of monoisotopic peaks and charge states	657:714	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	5	57	theme	mass	928:931	arg1	spectra					933:939	GAG tandem mass spectra	917:939	GAG tandem mass spectra	917:939	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	10	58	theme	peak	1731:1734	arg1	step					1800:1803	a subsequent step	1787:1803	a subsequent step for averagine-based methods	1787:1831	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	10	58	theme	peak	1731:1734	arg1	annotation					1756:1765	peak isotope composition annotation	1731:1765	peak isotope composition annotation	1731:1765	GAGfinder also performs peak isotope composition annotation, which is typically a subsequent step for averagine-based methods.					
29615495	5	59	theme	variable	1008:1015	arg1	sulfation					1017:1025	the variable sulfation	1004:1025	the variable sulfation of GAG disaccharide units	1004:1051	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	3	60	theme	saccharides	502:512	arg1	structures					484:493	the structures	480:493	the structures of GAG saccharides	480:512	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	4	61	theme	charge	702:707	arg1	states					709:714	charge states	702:714	charge states	702:714	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	8	62	theme	given	1395:1399	arg1	composition					1413:1423	a given product ion composition	1393:1423	a given product ion composition	1393:1423	We define peak finding as assigning experimental isotopic peaks directly to a given product ion composition, as opposed to deconvolution or peak picking, which are terms more accurately describing the existing methods previously mentioned.					
29615495	0	63	dep	Finding	18:24	arg1	Assignment					52:61	Assignment	52:61	Assignment	52:61	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	2	64	theme	GAG	272:274	arg1	length					276:281	GAG length and sulfation patterns	272:304	length	276:281	GAG length and sulfation patterns impact disease etiology, cellular signaling, and structural support for cells.					
29615495	4	65	theme	compound	794:801	arg1	class					803:807	the compound class	790:807	the compound class being analyzed	790:822	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	3	66	theme	tandem	549:554	arg1	spectra					561:567	tandem mass spectra	549:567	tandem mass spectra	549:567	We and others have demonstrated the usefulness of tandem mass spectrometry (MS2) for assigning the structures of GAG saccharides; however, manual interpretation of tandem mass spectra is time-consuming, so computational methods must be employed.					
29615495	1	67	theme	variable	227:234	arg1	patterns					246:253	variable sulfation patterns	227:253	variable sulfation patterns	227:253	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	5	68	theme	single	952:957	arg1	block					976:980	a single average building block	950:980	a single average building block	950:980	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	5	69	theme	GAG	1030:1032	arg1	units					1047:1051	GAG disaccharide units	1030:1051	GAG disaccharide units	1030:1051	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	4	70	theme	building	772:779	arg1	block					781:785	the average building block	760:785	the average building block of the compound class being analyzed	760:822	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	11	71	with	ProteomeXchange	1857:1871	arg1	identifier					1878:1887	identifier PXD009101	1878:1897	identifier PXD009101	1878:1897	Data are available via ProteomeXchange with identifier PXD009101.					
29615495	2	72	theme	cellular	331:338	arg1	signaling					340:348	cellular signaling	331:348	cellular signaling	331:348	GAG length and sulfation patterns impact disease etiology, cellular signaling, and structural support for cells.					
29615495	1	73	theme	sulfation	236:244	arg1	patterns					246:253	variable sulfation patterns	227:253	variable sulfation patterns	227:253	Glycosaminoglycans (GAGs) covalently linked to proteoglycans (PGs) are characterized by repeating disaccharide units and variable sulfation patterns along the chain.					
29615495	0	74	theme	Glycosaminoglycan	67:83	arg1	Spectra					97:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Glycosaminoglycan Tandem Mass Spectra	67:103	Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.					
29615495	5	75	theme	disaccharide	1034:1045	arg1	units					1047:1051	GAG disaccharide units	1030:1051	GAG disaccharide units	1030:1051	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	6	76	theme	GAG	1162:1164	arg1	saccharides					1166:1176	GAG saccharides	1162:1176	GAG saccharides	1162:1176	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	7	77	theme	peak	1261:1264	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	77	theme	peak	1261:1264	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	4	78	theme	monoisotopic	679:690	arg1	peaks					692:696	monoisotopic peaks	679:696	monoisotopic peaks	679:696	In the proteomics domain, the identification of monoisotopic peaks and charge states relies on algorithms that use averagine, or the average building block of the compound class being analyzed.					
29615495	5	79	theme	average	959:965	arg1	block					976:980	a single average building block	950:980	a single average building block	950:980	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
29615495	7	80	theme	tandem	1240:1245	arg1	GAGfinder					1219:1227	GAGfinder	1219:1227	GAGfinder	1219:1227	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	7	80	theme	tandem	1240:1245	arg1	algorithm					1274:1282	the first tandem mass spectrum peak finding algorithm	1230:1282	the first tandem mass spectrum peak finding algorithm developed specifically for GAGs	1230:1314	To address these problems, we developed GAGfinder, the first tandem mass spectrum peak finding algorithm developed specifically for GAGs.					
29615495	6	81	from	necessary	1073:1081	arg1	addition					1057:1064	addition	1057:1064	addition	1057:1064	In addition, it is necessary to assign product ion isotope patterns to interpret the tandem mass spectra of GAG saccharides.					
29615495	5	82	theme	peptide	877:883	arg1	spectra					885:891	peptide spectra	877:891	peptide spectra	877:891	Although these methods perform well for protein and peptide spectra, they perform poorly on GAG tandem mass spectra, because a single average building block does not characterize the variable sulfation of GAG disaccharide units.					
31776339	6	0	theme	cell-free	986:994	arg1	system					996:1001	a one-pot cell-free system	976:1001	a one-pot cell-free system	976:1001	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	6	1	theme	acid	1059:1062	arg1	motifs					1064:1069	minimal sialic acid motifs	1044:1069	minimal sialic acid motifs in glycoengineered Escherichia coli	1044:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	0	2	theme	pathways	84:91	arg1	construction					46:57	modular construction	38:57	modular construction of protein glycosylation pathways	38:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	4	3	theme	cell-free	557:565	arg1	glycosyltransferases					579:598	cell-free synthesized glycosyltransferases	557:598	cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase	557:693	In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase.					
31776339	7	4	theme	glycoengineering	1205:1220	arg1	applications					1222:1233	possible new glycoengineering applications	1192:1233	possible new glycoengineering applications	1192:1233	We anticipate that these methods and pathways will facilitate glycoscience and make possible new glycoengineering applications.					
31776339	6	5	with	candidate	928:936	arg1	motif					967:971	an α-galactose adjuvant motif	943:971	an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli	943:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	5	6	theme	putative	741:748	arg1	pathways					772:779	37 putative protein glycosylation pathways	738:779	37 putative protein glycosylation pathways	738:779	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	6	7	theme	selected	878:885	arg1	pathways					887:894	selected pathways	878:894	selected pathways	878:894	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	4	8	theme	glycosylation	497:509	arg1	pathways					511:518	glycosylation pathways	497:518	glycosylation pathways	497:518	In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase.					
31776339	5	9	theme	protein	750:756	arg1	pathways					772:779	37 putative protein glycosylation pathways	738:779	37 putative protein glycosylation pathways	738:779	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	6	10	with	regions	1031:1037	arg1	motifs					1064:1069	minimal sialic acid motifs	1044:1069	minimal sialic acid motifs in glycoengineered Escherichia coli	1044:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	4	11	theme	protein	651:657	arg1	targets					659:665	protein targets	651:665	protein targets	651:665	In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase.					
31776339	3	12	theme	versatile	364:372	arg1	platform					384:391	a modular, versatile cell-free platform	353:391	platform	384:391	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	6	13	from	motifs	1064:1069	arg1	coli					1102:1105	glycoengineered Escherichia coli	1074:1105	glycoengineered Escherichia coli	1074:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	1	14	theme	beneficial	188:197	arg1	properties					199:208	beneficial properties	188:208	beneficial properties	188:208	Glycosylation plays important roles in cellular function and endows protein therapeutics with beneficial properties.					
31776339	3	15	theme	cell-free	374:382	arg1	platform					384:391	a modular, versatile cell-free platform	353:391	platform	384:391	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	6	16	theme	adjuvant	958:965	arg1	motif					967:971	an α-galactose adjuvant motif	943:971	an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli	943:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	3	17	dep	in	437:438	arg1	vitro					440:444	vitro	440:444	vitro	440:444	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	0	18	theme	cell-free	2:10	arg1	platform					25:32	A cell-free biosynthesis platform	0:32	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways	0:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	6	19	theme	α-galactose	946:956	arg1	motif					967:971	an α-galactose adjuvant motif	943:971	an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli	943:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	0	20	theme	glycosylation	70:82	arg1	pathways					84:91	protein glycosylation pathways	62:91	protein glycosylation pathways	62:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	6	21	theme	one-pot	978:984	arg1	system					996:1001	a one-pot cell-free system	976:1001	a one-pot cell-free system	976:1001	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	6	22	theme	sialic	1052:1057	arg1	motifs					1064:1069	minimal sialic acid motifs	1044:1069	minimal sialic acid motifs in glycoengineered Escherichia coli	1044:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	3	23	theme	glycosylation	397:409	arg1	assembly					419:426	glycosylation pathway assembly	397:426	glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME)	397:479	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	6	24	theme	minimal	1044:1050	arg1	motifs					1064:1069	minimal sialic acid motifs	1044:1069	minimal sialic acid motifs in glycoengineered Escherichia coli	1044:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	1	25	theme	important	114:122	arg1	roles					124:128	important roles	114:128	important roles	114:128	Glycosylation plays important roles in cellular function and endows protein therapeutics with beneficial properties.					
31776339	3	26	theme	pathway	411:417	arg1	assembly					419:426	glycosylation pathway assembly	397:426	glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME)	397:479	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	5	27	theme	unique	794:799	arg1	motifs					808:813	23 unique glycan motifs	791:813	23 unique glycan motifs	791:813	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	0	28	theme	modular	38:44	arg1	construction					46:57	modular construction	38:57	modular construction of protein glycosylation pathways	38:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	4	29	theme	glucose	621:627	arg1	primer					629:634	a glucose primer	619:634	a glucose primer installed onto protein targets by an N-glycosyltransferase	619:693	In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase.					
31776339	5	30	theme	glycan	801:806	arg1	motifs					808:813	23 unique glycan motifs	791:813	23 unique glycan motifs	791:813	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	2	31	from	structures	292:301	arg1	proteins					306:313	proteins	306:313	proteins	306:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	0	32	theme	biosynthesis	12:23	arg1	platform					25:32	A cell-free biosynthesis platform	0:32	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways	0:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	6	33	theme	constant	1022:1029	arg1	regions					1031:1037	human antibody constant regions	1007:1037	human antibody constant regions	1007:1037	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	5	34	theme	motifs	808:813	arg1	18					816:817	18	816:817	18	816:817	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	5	34	theme	motifs	808:813	arg1	motifs					808:813	23 unique glycan motifs	791:813	23 unique glycan motifs	791:813	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	6	35	from	motif	967:971	arg1	regions					1031:1037	human antibody constant regions	1007:1037	human antibody constant regions	1007:1037	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	6	35	from	motif	967:971	arg1	system					996:1001	a one-pot cell-free system	976:1001	a one-pot cell-free system	976:1001	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	3	36	theme	rapid	431:435	arg1	mixing					446:451	rapid in vitro mixing	431:451	rapid in vitro mixing	431:451	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	4	37	theme	synthesized	567:577	arg1	glycosyltransferases					579:598	cell-free synthesized glycosyltransferases	557:598	cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase	557:693	In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltransferase.					
31776339	5	38	theme	glycosylation	758:770	arg1	pathways					772:779	37 putative protein glycosylation pathways	738:779	37 putative protein glycosylation pathways	738:779	We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins.					
31776339	6	39	theme	vaccine	920:926	arg1	candidate					928:936	a protein vaccine candidate	910:936	a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli	910:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	3	40	theme	in	437:438	arg1	mixing					446:451	rapid in vitro mixing	431:451	rapid in vitro mixing	431:451	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	6	41	theme	human	1007:1011	arg1	regions					1031:1037	human antibody constant regions	1007:1037	human antibody constant regions	1007:1037	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	6	42	theme	protein	912:918	arg1	candidate					928:936	a protein vaccine candidate	910:936	a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli	910:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	7	43	theme	possible	1192:1199	arg1	applications					1222:1233	possible new glycoengineering applications	1192:1233	possible new glycoengineering applications	1192:1233	We anticipate that these methods and pathways will facilitate glycoscience and make possible new glycoengineering applications.					
31776339	1	44	theme	cellular	133:140	arg1	function					142:149	cellular function	133:149	cellular function	133:149	Glycosylation plays important roles in cellular function and endows protein therapeutics with beneficial properties.					
31776339	2	45	theme	biosynthetic	233:244	arg1	pathways					246:253	constructing biosynthetic pathways	220:253	constructing biosynthetic pathways to study and engineer precise glycan structures on proteins	220:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	6	46	with	system	996:1001	arg1	motifs					1064:1069	minimal sialic acid motifs	1044:1069	minimal sialic acid motifs in glycoengineered Escherichia coli	1044:1105	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
31776339	2	47	theme	constructing	220:231	arg1	pathways					246:253	constructing biosynthetic pathways	220:253	constructing biosynthetic pathways to study and engineer precise glycan structures on proteins	220:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	7	48	theme	new	1201:1203	arg1	applications					1222:1233	possible new glycoengineering applications	1192:1233	possible new glycoengineering applications	1192:1233	We anticipate that these methods and pathways will facilitate glycoscience and make possible new glycoengineering applications.					
31776339	3	49	theme	modular	355:361	arg1	platform					384:391	a modular, versatile cell-free platform	353:391	platform	384:391	Here, we report a modular, versatile cell-free platform for glycosylation pathway assembly by rapid in vitro mixing and expression (GlycoPRIME).					
31776339	2	50	theme	glycan	285:290	arg1	structures					292:301	precise glycan structures	277:301	precise glycan structures on proteins	277:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	2	51	dep	pathways	246:253	arg1	engineer					268:275	engineer	268:275	engineer precise glycan structures on proteins	268:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	2	51	dep	pathways	246:253	arg1	study					258:262	study	258:262	study	258:262	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	0	52	theme	protein	62:68	arg1	pathways					84:91	protein glycosylation pathways	62:91	protein glycosylation pathways	62:91	A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.					
31776339	2	53	theme	precise	277:283	arg1	structures					292:301	precise glycan structures	277:301	precise glycan structures on proteins	277:313	However, constructing biosynthetic pathways to study and engineer precise glycan structures on proteins remains a bottleneck.					
31776339	1	54	theme	protein	162:168	arg1	therapeutics					170:181	protein therapeutics	162:181	protein therapeutics	162:181	Glycosylation plays important roles in cellular function and endows protein therapeutics with beneficial properties.					
31776339	6	55	theme	antibody	1013:1020	arg1	regions					1031:1037	human antibody constant regions	1007:1037	human antibody constant regions	1007:1037	We use selected pathways to synthesize a protein vaccine candidate with an α-galactose adjuvant motif in a one-pot cell-free system and human antibody constant regions with minimal sialic acid motifs in glycoengineered Escherichia coli.					
30624066	2	0	theme	mobility	377:384	arg1	separation					386:395	ion mobility separation	373:395	ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS)	373:440	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	2	1	theme	GBP-GP	475:480	arg1	binding					482:488	GBP-GP binding	475:488	GBP-GP binding in solution	475:500	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	1	2	gly	glycoproteins	277:289	arg1	GPs					292:294	GPs	292:294	GPs	292:294	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	2	gly	glycoproteins	277:289	arg1	glycoproteins					277:289	glycoproteins	277:289	glycoproteins (GPs)	277:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	2	3	theme	ion	373:375	arg1	separation					386:395	ion mobility separation	373:395	ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS)	373:440	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	8	4	theme	reported	1543:1550	arg1	affinities					1561:1570	the reported apparent affinities	1539:1570	the reported apparent affinities of hGal-3 for serum GPs and their N-glycans	1539:1614	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	8	5	theme	3	1492:1492	arg1	×					1494:1494	×	1494:1494	×	1494:1494	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	2	6	theme	catch-and-release	322:338	arg1	CaR					341:343	CaR	341:343	CaR	341:343	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	2	6	theme	catch-and-release	322:338	arg1	assay					353:357	the catch-and-release (CaR)-ESI-MS assay	318:357	the catch-and-release (CaR)-ESI-MS assay	318:357	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	3	7	theme	GBP	538:540	arg1	Ka					527:528	Ka	527:528	Ka	527:528	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	3	7	theme	GBP	538:540	arg1	affinity					516:523	The apparent affinity	503:523	The apparent affinity ( Ka) of the GBP for the GP	503:551	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	1	8	theme	glycan-mediated	191:205	arg1	interactions					207:218	glycan-mediated interactions	191:218	glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	191:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	9	theme	multipronged	100:111	arg1	ESI-MS					156:161	ESI-MS	156:161	ESI-MS	156:161	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	9	theme	multipronged	100:111	arg1	spectrometry					142:153	A multipronged electrospray ionization mass spectrometry	98:153	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	98:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	7	10	theme	negative	1382:1389	arg1	control					1391:1397	a negative control	1380:1397	a negative control	1380:1397	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	7	10	theme	negative	1382:1389	arg1	protein					1355:1361	a noninteracting reference protein	1328:1361	a noninteracting reference protein	1328:1361	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	4	11	theme	free	673:676	arg1	oligosaccharides					678:693	free oligosaccharides	673:693	free oligosaccharides from the GP	673:705	Finally, N-glycans, enzymatically released as free oligosaccharides from the GP, are screened against the GBP using ESI-MS to identify the glycans that are recognized by the GBP.					
30624066	9	12	theme	compositions	1756:1767	arg1	total					1723:1727	a total	1721:1727	a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures	1721:1825	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	7	13	theme	noninteracting	1330:1343	arg1	control					1391:1397	a negative control	1380:1397	a negative control	1380:1397	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	7	13	theme	noninteracting	1330:1343	arg1	protein					1355:1361	a noninteracting reference protein	1328:1361	a noninteracting reference protein	1328:1361	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	6	14	theme	known	1012:1016	arg1	α-2-macroglobulin					1192:1208	α-2-macroglobulin	1192:1208	α-2-macroglobulin (α2M)	1192:1214	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	14	theme	known	1012:1016	arg1	1-1					1176:1178	haptoglobin phenotype 1-1	1154:1178	haptoglobin phenotype 1-1 (Hp1-1)	1154:1186	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	14	theme	known	1012:1016	arg1	interactions					1018:1029	the known interactions	1008:1029	the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP)	1008:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	15	theme	α-1-acid	1125:1132	arg1	AGP					1148:1150	AGP	1148:1150	AGP	1148:1150	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	15	theme	α-1-acid	1125:1132	arg1	GPs					1120:1122	three human serum GPs	1102:1122	three human serum GPs	1102:1122	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	15	theme	α-1-acid	1125:1132	arg1	glycoprotein					1134:1145	α-1-acid glycoprotein	1125:1145	α-1-acid glycoprotein (AGP)	1125:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	1	16	theme	water-soluble	228:240	arg1	GBPs					267:270	GBPs	267:270	GBPs	267:270	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	16	theme	water-soluble	228:240	arg1	proteins					257:264	water-soluble glycan-binding proteins	228:264	water-soluble glycan-binding proteins (GBPs)	228:271	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	2	17	theme	release	411:417	arg1	CaRIMS-ESI-MS					427:439	CaRIMS-ESI-MS	427:439	CaRIMS-ESI-MS	427:439	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	2	17	theme	release	411:417	arg1	"					418:418	GBP "release"	406:418	GBP "release" (i.e., CaRIMS-ESI-MS)	406:440	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	8	18	theme	105	1496:1498	arg1	×					1494:1494	×	1494:1494	×	1494:1494	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	2	19	dep	CaRIMS-ESI-MS	427:439	arg1	i.e.					421:424	i.e.	421:424	i.e.	421:424	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	8	20	theme	hGal-3	1575:1580	arg1	affinities					1561:1570	the reported apparent affinities	1539:1570	the reported apparent affinities of hGal-3 for serum GPs and their N-glycans	1539:1614	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	6	21	theme	C-terminal	1041:1050	arg1	fragment					1059:1066	a C-terminal domain fragment	1039:1066	a C-terminal domain fragment of human galectin-3 (hGal-3C)	1039:1096	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	0	22	theme	ESI-MS	13:18	arg1	Approach					20:27	ESI-MS Approach	13:27	ESI-MS Approach	13:27	Multipronged ESI-MS Approach for Studying Glycan-Binding Protein Interactions with Glycoproteins.					
30624066	8	23	theme	apparent	1552:1559	arg1	affinities					1561:1570	the reported apparent affinities	1539:1570	the reported apparent affinities of hGal-3 for serum GPs and their N-glycans	1539:1614	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	2	24	theme	-ESI-MS	345:351	arg1	CaR					341:343	CaR	341:343	CaR	341:343	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	2	24	theme	-ESI-MS	345:351	arg1	assay					353:357	the catch-and-release (CaR)-ESI-MS assay	318:357	the catch-and-release (CaR)-ESI-MS assay	318:357	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	10	25	theme	M-1	1980:1982	arg1	affinities					1964:1973	affinities	1964:1973	affinities ≥104 M-1	1964:1982	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	2	26	theme	GBP	406:408	arg1	CaRIMS-ESI-MS					427:439	CaRIMS-ESI-MS	427:439	CaRIMS-ESI-MS	427:439	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	2	26	theme	GBP	406:408	arg1	"					418:418	GBP "release"	406:418	GBP "release" (i.e., CaRIMS-ESI-MS)	406:440	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	1	27	theme	electrospray	113:124	arg1	ESI-MS					156:161	ESI-MS	156:161	ESI-MS	156:161	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	27	theme	electrospray	113:124	arg1	spectrometry					142:153	A multipronged electrospray ionization mass spectrometry	98:153	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	98:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	8	28	theme	serum	1586:1590	arg1	GPs					1592:1594	serum GPs	1586:1594	serum GPs	1586:1594	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	1	29	theme	glycan-binding	242:255	arg1	GBPs					267:270	GBPs	267:270	GBPs	267:270	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	29	theme	glycan-binding	242:255	arg1	proteins					257:264	water-soluble glycan-binding proteins	228:264	water-soluble glycan-binding proteins (GBPs)	228:271	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	9	30	theme	N-glycan	1631:1638	arg1	libraries					1640:1648	the N-glycan libraries	1627:1648	the N-glycan libraries of each of the GPs against hGal-3C	1627:1683	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	7	31	theme	reference	1345:1353	arg1	control					1391:1397	a negative control	1380:1397	a negative control	1380:1397	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	7	31	theme	reference	1345:1353	arg1	protein					1355:1361	a noninteracting reference protein	1328:1361	a noninteracting reference protein	1328:1361	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	1	32	theme	ionization	126:135	arg1	ESI-MS					156:161	ESI-MS	156:161	ESI-MS	156:161	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	32	theme	ionization	126:135	arg1	spectrometry					142:153	A multipronged electrospray ionization mass spectrometry	98:153	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	98:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	5	33	theme	compositional	919:931	arg1	isomers					933:939	compositional isomers	919:939	compositional isomers	919:939	Measurements performed at multiple GBP concentrations allow for the affinities of released N-glycans (grouped as compositional isomers) to be ranked.					
30624066	7	34	theme	hGal-3C	1237:1243	arg1	binding					1226:1232	Specific binding	1217:1232	Specific binding of hGal-3C to each GP	1217:1254	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	6	35	theme	serum	1114:1118	arg1	GPs					1120:1122	three human serum GPs	1102:1122	three human serum GPs	1102:1122	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	35	theme	serum	1114:1118	arg1	glycoprotein					1134:1145	α-1-acid glycoprotein	1125:1145	α-1-acid glycoprotein (AGP)	1125:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	2	36	from	binding	482:488	arg1	solution					493:500	solution	493:500	solution	493:500	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	1	37	theme	mass	137:140	arg1	ESI-MS					156:161	ESI-MS	156:161	ESI-MS	156:161	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	1	37	theme	mass	137:140	arg1	spectrometry					142:153	A multipronged electrospray ionization mass spectrometry	98:153	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	98:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	3	38	theme	apparent	507:514	arg1	Ka					527:528	Ka	527:528	Ka	527:528	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	3	38	theme	apparent	507:514	arg1	affinity					516:523	The apparent affinity	503:523	The apparent affinity ( Ka) of the GBP for the GP	503:551	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	0	39	theme	Glycan-Binding	42:55	arg1	Interactions					65:76	Glycan-Binding Protein Interactions	42:76	Glycan-Binding Protein Interactions with Glycoproteins	42:95	Multipronged ESI-MS Approach for Studying Glycan-Binding Protein Interactions with Glycoproteins.					
30624066	8	40	theme	4	1463:1463	arg1	×					1465:1465	×	1465:1465	×	1465:1465	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	10	41	theme	hGal-3C	1891:1897	arg1	concentrations					1899:1912	two different hGal-3C concentrations	1877:1912	two different hGal-3C concentrations	1877:1912	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	9	42	theme	different	1735:1743	arg1	compositions					1756:1767	20 different saccharide compositions	1732:1767	20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures	1732:1825	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	5	43	theme	GBP	841:843	arg1	concentrations					845:858	multiple GBP concentrations	832:858	multiple GBP concentrations	832:858	Measurements performed at multiple GBP concentrations allow for the affinities of released N-glycans (grouped as compositional isomers) to be ranked.					
30624066	6	44	theme	domain	1052:1057	arg1	fragment					1059:1066	a C-terminal domain fragment	1039:1066	a C-terminal domain fragment of human galectin-3 (hGal-3C)	1039:1096	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	10	45	theme	measurements	1851:1862	arg1	results					1832:1838	The results	1828:1838	The results of binding measurements performed at two different hGal-3C concentrations	1828:1912	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	5	46	theme	multiple	832:839	arg1	concentrations					845:858	multiple GBP concentrations	832:858	multiple GBP concentrations	832:858	Measurements performed at multiple GBP concentrations allow for the affinities of released N-glycans (grouped as compositional isomers) to be ranked.					
30624066	3	47	theme	ligand-ESI-MS	598:610	arg1	assay					620:624	the competitive proxy ligand-ESI-MS binding assay	576:624	the competitive proxy ligand-ESI-MS binding assay	576:624	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	9	48	theme	tetra-antennary	1800:1814	arg1	structures					1816:1825	tetra-antennary structures	1800:1825	tetra-antennary structures	1800:1825	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	6	49	gly	glycoprotein	1134:1145	arg1	AGP					1148:1150	AGP	1148:1150	AGP	1148:1150	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	49	gly	glycoprotein	1134:1145	arg1	GPs					1120:1122	three human serum GPs	1102:1122	three human serum GPs	1102:1122	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	49	gly	glycoprotein	1134:1145	arg1	glycoprotein					1134:1145	α-1-acid glycoprotein	1125:1145	α-1-acid glycoprotein (AGP)	1125:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	8	50	theme	105	1467:1469	arg1	×					1465:1465	×	1465:1465	×	1465:1465	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	3	51	theme	binding	612:618	arg1	assay					620:624	the competitive proxy ligand-ESI-MS binding assay	576:624	the competitive proxy ligand-ESI-MS binding assay	576:624	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	9	52	gly	sialylated	1774:1783	arg1	bi-					1785:1787	sialylated bi-	1774:1787	sialylated bi-	1774:1787	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	6	53	theme	galectin-3	1077:1086	arg1	fragment					1059:1066	a C-terminal domain fragment	1039:1066	a C-terminal domain fragment of human galectin-3 (hGal-3C)	1039:1096	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	53	theme	galectin-3	1077:1086	arg1	GPs					1120:1122	three human serum GPs	1102:1122	three human serum GPs	1102:1122	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	53	theme	galectin-3	1077:1086	arg1	glycoprotein					1134:1145	α-1-acid glycoprotein	1125:1145	α-1-acid glycoprotein (AGP)	1125:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	1	54	theme	spectrometry	142:153	arg1	approach					164:171	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach	98:171	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs)	98:295	A multipronged electrospray ionization mass spectrometry (ESI-MS) approach for investigating glycan-mediated interactions between water-soluble glycan-binding proteins (GBPs) and glycoproteins (GPs) is described.					
30624066	0	55	theme	Protein	57:63	arg1	Interactions					65:76	Glycan-Binding Protein Interactions	42:76	Glycan-Binding Protein Interactions with Glycoproteins	42:95	Multipronged ESI-MS Approach for Studying Glycan-Binding Protein Interactions with Glycoproteins.					
30624066	8	56	theme	2	1476:1476	arg1	×					1478:1478	×	1478:1478	×	1478:1478	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	6	57	theme	phenotype	1166:1174	arg1	1-1					1176:1178	haptoglobin phenotype 1-1	1154:1178	haptoglobin phenotype 1-1 (Hp1-1)	1154:1186	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	57	theme	phenotype	1166:1174	arg1	interactions					1018:1029	the known interactions	1008:1029	the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP)	1008:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	57	theme	phenotype	1166:1174	arg1	Hp1-1					1181:1185	Hp1-1	1181:1185	Hp1-1	1181:1185	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	7	58	theme	Specific	1217:1224	arg1	binding					1226:1232	Specific binding	1217:1232	Specific binding of hGal-3C to each GP	1217:1254	Specific binding of hGal-3C to each GP was successfully detected by CaRIMS-ESI-MS; no binding was detected for a noninteracting reference protein, which served as a negative control.					
30624066	4	59	from	GP	704:705	arg1	oligosaccharides					678:693	free oligosaccharides	673:693	free oligosaccharides from the GP	673:705	Finally, N-glycans, enzymatically released as free oligosaccharides from the GP, are screened against the GBP using ESI-MS to identify the glycans that are recognized by the GBP.					
30624066	6	60	theme	haptoglobin	1154:1164	arg1	1-1					1176:1178	haptoglobin phenotype 1-1	1154:1178	haptoglobin phenotype 1-1 (Hp1-1)	1154:1186	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	60	theme	haptoglobin	1154:1164	arg1	interactions					1018:1029	the known interactions	1008:1029	the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP)	1008:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	60	theme	haptoglobin	1154:1164	arg1	Hp1-1					1181:1185	Hp1-1	1181:1185	Hp1-1	1181:1185	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	3	61	theme	competitive	580:590	arg1	assay					620:624	the competitive proxy ligand-ESI-MS binding assay	576:624	the competitive proxy ligand-ESI-MS binding assay	576:624	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	5	62	theme	released	888:895	arg1	N-glycans					897:905	released N-glycans	888:905	released N-glycans	888:905	Measurements performed at multiple GBP concentrations allow for the affinities of released N-glycans (grouped as compositional isomers) to be ranked.					
30624066	6	63	theme	human	1108:1112	arg1	GPs					1120:1122	three human serum GPs	1102:1122	three human serum GPs	1102:1122	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	63	theme	human	1108:1112	arg1	glycoprotein					1134:1145	α-1-acid glycoprotein	1125:1145	α-1-acid glycoprotein (AGP)	1125:1151	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	8	64	theme	105	1480:1482	arg1	×					1478:1478	×	1478:1478	×	1478:1478	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	8	65	with	consistent	1523:1532	arg1	affinities					1561:1570	the reported apparent affinities	1539:1570	the reported apparent affinities of hGal-3 for serum GPs and their N-glycans	1539:1614	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	9	66	with	compositions	1756:1767	arg1	tri-					1790:1793	tri-	1790:1793	tri-	1790:1793	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	9	66	with	compositions	1756:1767	arg1	bi-					1785:1787	sialylated bi-	1774:1787	sialylated bi-	1774:1787	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	9	66	with	compositions	1756:1767	arg1	structures					1816:1825	tetra-antennary structures	1800:1825	tetra-antennary structures	1800:1825	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	3	67	theme	proxy	592:596	arg1	assay					620:624	the competitive proxy ligand-ESI-MS binding assay	576:624	the competitive proxy ligand-ESI-MS binding assay	576:624	The apparent affinity ( Ka) of the GBP for the GP is then measured using the competitive proxy ligand-ESI-MS binding assay.					
30624066	0	68	with	Interactions	65:76	arg1	Glycoproteins					83:95	Glycoproteins	83:95	Glycoproteins	83:95	Multipronged ESI-MS Approach for Studying Glycan-Binding Protein Interactions with Glycoproteins.					
30624066	5	69	theme	N-glycans	897:905	arg1	affinities					874:883	the affinities	870:883	the affinities of released N-glycans (grouped as compositional isomers)	870:940	Measurements performed at multiple GBP concentrations allow for the affinities of released N-glycans (grouped as compositional isomers) to be ranked.					
30624066	9	70	theme	each	1653:1656	arg1	libraries					1640:1648	the N-glycan libraries	1627:1648	the N-glycan libraries of each of the GPs against hGal-3C	1627:1683	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	9	71	theme	sialylated	1774:1783	arg1	bi-					1785:1787	sialylated bi-	1774:1787	sialylated bi-	1774:1787	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	9	72	theme	saccharide	1745:1754	arg1	compositions					1756:1767	20 different saccharide compositions	1732:1767	20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures	1732:1825	Screening the N-glycan libraries of each of the GPs against hGal-3C identified ligands corresponding to a total of 20 different saccharide compositions with sialylated bi-, tri-, and tetra-antennary structures.					
30624066	10	73	theme	different	1881:1889	arg1	concentrations					1899:1912	two different hGal-3C concentrations	1877:1912	two different hGal-3C concentrations	1877:1912	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	2	74	theme	prior	397:401	arg1	separation					386:395	ion mobility separation	373:395	ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS)	373:440	First, the catch-and-release (CaR)-ESI-MS assay, carried with ion mobility separation prior to GBP "release" (i.e., CaRIMS-ESI-MS), is employed to rapidly identify GBP-GP binding in solution.					
30624066	10	75	theme	binding	1843:1849	arg1	measurements					1851:1862	binding measurements	1843:1862	binding measurements performed at two different hGal-3C concentrations	1843:1912	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	6	76	theme	approach	978:985	arg1	Implementation					956:969	Implementation	956:969	Implementation of the approach	956:985	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	10	77	theme	N-glycan	1939:1946	arg1	ligands					1948:1954	the N-glycan ligands	1935:1954	the N-glycan ligands	1935:1954	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	8	78	theme	proxy	1419:1423	arg1	ligand-ESI-MS					1425:1437	proxy ligand-ESI-MS	1419:1437	proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively)	1419:1517	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	6	79	theme	human	1071:1075	arg1	hGal-3C					1089:1095	hGal-3C	1089:1095	hGal-3C	1089:1095	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	6	79	theme	human	1071:1075	arg1	galectin-3					1077:1086	human galectin-3	1071:1086	human galectin-3 (hGal-3C)	1071:1096	Implementation of the approach is illustrated using the known interactions between a C-terminal domain fragment of human galectin-3 (hGal-3C) and three human serum GPs, α-1-acid glycoprotein (AGP), haptoglobin phenotype 1-1 (Hp1-1) and α-2-macroglobulin (α2M).					
30624066	10	80	theme	≥104	1975:1978	arg1	M-1					1980:1982	M-1	1980:1982	M-1	1980:1982	The results of binding measurements performed at two different hGal-3C concentrations revealed that all of the N-glycan ligands exhibit affinities ≥104 M-1.					
30624066	8	81	dep	AGP	1443:1445	arg1	M-1					1484:1486	2 × 105 M-1	1476:1486	2 × 105 M-1	1476:1486	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	8	81	dep	AGP	1443:1445	arg1	M-1					1471:1473	4 × 105 M-1	1463:1473	4 × 105 M-1	1463:1473	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30624066	8	81	dep	AGP	1443:1445	arg1	M-1					1500:1502	3 × 105 M-1	1492:1502	3 × 105 M-1	1492:1502	The Ka measured by proxy ligand-ESI-MS for AGP, Hp1-1 and α2M (4 × 105 M-1, 2 × 105 M-1 and 3 × 105 M-1, respectively) are consistent with the reported apparent affinities of hGal-3 for serum GPs and their N-glycans.					
30325416	3	0	theme	antibodies	534:543	arg1	design					512:517	the design	508:517	the design of therapeutic antibodies	508:543	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	5	1	from	simulations	985:995	arg1	excess					1000:1005	excess	1000:1005	excess of 62 μs of cumulative simulation time	1000:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	3	2	theme	antibody	574:581	arg1	response					596:603	the antibody inflammatory response	570:603	the antibody inflammatory response	570:603	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	5	3	theme	N-glycan	1222:1229	arg1	species					1231:1237	the larger N-glycan species	1211:1237	the larger N-glycan species commonly found in the Fc region of human IgGs	1211:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	4	4	theme	intrinsic	860:868	arg1	propensity					885:894	its intrinsic conformational propensity	856:894	its intrinsic conformational propensity	856:894	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	2	5	gly	glycoforms	265:274	arg1	Fc					262:263	the Fc glycoforms	258:274	the Fc glycoforms	258:274	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	5	6	from	chitobiose	1197:1206	arg1	protein					1178:1184	the protein	1174:1184	the protein	1174:1184	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	2	7	theme	Fc	262:263	arg1	glycoforms					265:274	the Fc glycoforms	258:274	the Fc glycoforms	258:274	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	6	8	theme	folded	1523:1528	arg1	conformation					1530:1541	a folded conformation	1521:1541	a folded conformation	1521:1541	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	7	9	theme	outstretched	1783:1794	arg1	arm					1803:1805	the more accessible, outstretched α(1-3) arm	1762:1805	arm	1803:1805	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	7	10	theme	differential	1645:1656	arg1	recognition					1658:1668	a differential recognition	1643:1668	a differential recognition of positional isomers in glycan array data	1643:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	7	11	theme	accessible	1771:1780	arg1	arm					1803:1805	the more accessible, outstretched α(1-3) arm	1762:1805	arm	1803:1805	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	1	12	gly	glycosylation	114:126	arg1	IgGs					156:159	human immunoglobulins G (IgGs)	131:160	human immunoglobulins G (IgGs)	131:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	6	13	gly	sialylation	1336:1346	arg1	N-glycans					1401:1409	the unlinked N-glycans	1388:1409	the unlinked N-glycans	1388:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	3	14	dep	reverse	562:568	arg1	affects					410:416	affects	410:416	affects	410:416	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	7	15	from	recognition	1658:1668	arg1	data					1708:1711	glycan array data	1695:1711	glycan array data	1695:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	6	16	theme	core	1314:1317	arg1	fucosylation					1319:1330	core fucosylation	1314:1330	core fucosylation	1314:1330	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	6	17	gly	fucosylation	1319:1330	arg1	N-glycans					1401:1409	the unlinked N-glycans	1388:1409	the unlinked N-glycans	1388:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	7	18	theme	experimental	1613:1624	arg1	evidence					1626:1633	recent experimental evidence	1606:1633	recent experimental evidence showing a differential recognition of positional isomers in glycan array data	1606:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	6	19	theme	α	1435:1435	arg1	arm					1442:1444	the α(1-6) arm	1431:1444	the α(1-6) arm	1431:1444	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	5	20	theme	study	940:944	arg1	results					916:922	the results	912:922	the results of a systematic study	912:944	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	21	theme	IgGs	1280:1283	arg1	region					1264:1269	the Fc region	1257:1269	the Fc region of human IgGs	1257:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	1	22	theme	immunoglobulins	137:151	arg1	IgGs					156:159	human immunoglobulins G (IgGs)	131:160	human immunoglobulins G (IgGs)	131:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	6	23	link	unlinked	1392:1399	arg1	N-glycans					1401:1409	the unlinked N-glycans	1388:1409	the unlinked N-glycans	1388:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	0	24	theme	N-glycans	72:80	arg1	dependence					22:31	Sequence-to-structure dependence	0:31	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.	0:109	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	2	25	theme	specific	239:246	arg1	nature					248:253	the specific nature	235:253	the specific nature of the Fc glycoforms	235:274	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	5	26	theme	Fc	1261:1262	arg1	region					1264:1269	the Fc region	1257:1269	the Fc region of human IgGs	1257:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	27	from	effect	1054:1059	arg1	dynamics					1094:1101	dynamics	1094:1101	dynamics	1094:1101	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	27	from	effect	1054:1059	arg1	structure					1080:1088	the structure	1076:1088	the structure	1076:1088	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	28	theme	biantennary	1135:1145	arg1	N-glycoforms					1147:1158	increasingly larger, complex biantennary N-glycoforms	1106:1158	increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs	1106:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	1	29	theme	Fc	111:112	arg1	glycosylation					114:126	Fc glycosylation	111:126	Fc glycosylation of human immunoglobulins G (IgGs)	111:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	0	30	theme	dynamics	95:102	arg1	study					104:108	a molecular dynamics study	83:108	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.	0:109	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	7	31	theme	array	1702:1706	arg1	data					1708:1711	glycan array data	1695:1711	glycan array data	1695:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	5	32	dep	protein	1178:1184	arg1	i.e.					1187:1190	i.e.	1187:1190	i.e.	1187:1190	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	3	33	theme	cytotoxicity	455:466	arg1	function					468:475	the antibody-dependent cell-mediated cytotoxicity function	418:475	the antibody-dependent cell-mediated cytotoxicity function	418:475	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	34	theme	antibody-dependent	422:439	arg1	cytotoxicity					455:466	the antibody-dependent cell-mediated cytotoxicity	418:466	the antibody-dependent cell-mediated cytotoxicity function	418:475	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	0	35	theme	Sequence-to-structure	0:20	arg1	dependence					22:31	Sequence-to-structure dependence	0:31	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.	0:109	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	5	36	theme	extensive	956:964	arg1	simulations					985:995	extensive molecular dynamics simulations	956:995	extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time	956:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	7	37	theme	positional	1673:1682	arg1	isomers					1684:1690	positional isomers	1673:1690	positional isomers	1673:1690	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	4	38	theme	structure-to-function	767:787	arg1	relationship					789:800	a structure-to-function relationship	765:800	a structure-to-function relationship	765:800	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	3	39	theme	obvious	483:489	arg1	repercussions					491:503	obvious repercussions	483:503	obvious repercussions in the design of therapeutic antibodies	483:543	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	5	40	theme	dynamics	976:983	arg1	simulations					985:995	extensive molecular dynamics simulations	956:995	extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time	956:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	4	41	theme	N-glycan	835:842	arg1	sequence					844:851	the N-glycan sequence	831:851	the N-glycan sequence	831:851	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	7	42	theme	sialyltransferase	1740:1756	arg1	preference					1726:1735	the preference	1722:1735	the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans	1722:1846	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	0	43	theme	IgG	45:47	arg1	N-glycans					72:80	isolated IgG Fc complex biantennary N-glycans	36:80	isolated IgG Fc complex biantennary N-glycans	36:80	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	5	44	theme	time	1041:1044	arg1	μs					1013:1014	62 μs	1010:1014	62 μs of cumulative simulation time	1010:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	7	45	theme	Fc-bound	1829:1836	arg1	N-glycans					1838:1846	both isolated, and Fc-bound N-glycans	1810:1846	both isolated, and Fc-bound N-glycans	1810:1846	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	3	46	theme	Fc-glycans	391:400	arg1	core-fucosylation					366:382	core-fucosylation	366:382	core-fucosylation of IgG Fc-glycans	366:400	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	5	47	dep	larger	1119:1124	arg1	complex					1127:1133	complex	1127:1133	complex	1127:1133	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	2	48	theme	inflammatory	327:338	arg1	properties					340:349	inflammatory properties	327:349	inflammatory properties	327:349	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	0	49	theme	complex	52:58	arg1	N-glycans					72:80	isolated IgG Fc complex biantennary N-glycans	36:80	isolated IgG Fc complex biantennary N-glycans	36:80	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	5	50	located	found	1248:1252	arg1	region					1264:1269	the Fc region	1257:1269	the Fc region of human IgGs	1257:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	50	located	found	1248:1252	arg2	species					1231:1237	the larger N-glycan species	1211:1237	the larger N-glycan species commonly found in the Fc region of human IgGs	1211:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	3	51	from	repercussions	491:503	arg1	design					512:517	the design	508:517	the design of therapeutic antibodies	508:543	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	6	52	theme	conformational	1470:1483	arg1	equilibrium					1485:1495	its conformational equilibrium	1466:1495	its conformational equilibrium from an outstretched to a folded conformation	1466:1541	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	5	53	theme	μs	1013:1014	arg1	excess					1000:1005	excess	1000:1005	excess of 62 μs of cumulative simulation time	1000:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	6	54	theme	N-glycans	1401:1409	arg1	dynamics					1376:1383	the intrinsic dynamics	1362:1383	the intrinsic dynamics of the unlinked N-glycans	1362:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	5	55	theme	cumulative	1019:1028	arg1	time					1041:1044	cumulative simulation time	1019:1044	cumulative simulation time	1019:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	3	56	theme	rheumatoid	719:728	arg1	arthritis					730:738	rheumatoid arthritis	719:738	rheumatoid arthritis	719:738	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	57	theme	therapeutic	522:532	arg1	antibodies					534:543	therapeutic antibodies	522:543	therapeutic antibodies	522:543	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	6	58	theme	arm	1442:1444	arg1	galactosylation					1412:1426	galactosylation	1412:1426	galactosylation of the α(1-6) arm	1412:1444	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	5	59	theme	larger	1215:1220	arg1	species					1231:1237	the larger N-glycan species	1211:1237	the larger N-glycan species commonly found in the Fc region of human IgGs	1211:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	7	60	theme	α	1796:1796	arg1	arm					1803:1805	the more accessible, outstretched α(1-3) arm	1762:1805	arm	1803:1805	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	2	61	theme	glycoforms	265:274	arg1	nature					248:253	the specific nature	235:253	the specific nature of the Fc glycoforms	235:274	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	6	62	theme	unlinked	1392:1399	arg1	N-glycans					1401:1409	the unlinked N-glycans	1388:1409	the unlinked N-glycans	1388:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	5	63	gly	N-glycoforms	1147:1158	arg1	biantennary					1135:1145	increasingly larger, complex biantennary N-glycoforms	1106:1158	increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs	1106:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	6	64	theme	intrinsic	1366:1374	arg1	dynamics					1376:1383	the intrinsic dynamics	1362:1383	the intrinsic dynamics of the unlinked N-glycans	1362:1409	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	2	65	theme	IgG	301:303	arg1	function					314:321	the IgG effector function	297:321	the IgG effector function	297:321	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	4	66	theme	conformational	870:883	arg1	propensity					885:894	its intrinsic conformational propensity	856:894	its intrinsic conformational propensity	856:894	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	3	67	theme	inflammatory	583:594	arg1	response					596:603	the antibody inflammatory response	570:603	the antibody inflammatory response	570:603	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	1	68	theme	structural	185:194	arg1	integrity					196:204	their structural integrity	179:204	their structural integrity	179:204	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	7	69	theme	recent	1606:1611	arg1	evidence					1626:1633	recent experimental evidence	1606:1633	recent experimental evidence showing a differential recognition of positional isomers in glycan array data	1606:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	3	70	theme	galactosylation	610:624	arg1	levels					626:631	galactosylation levels	610:631	galactosylation levels	610:631	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	5	71	theme	systematic	929:938	arg1	study					940:944	a systematic study	927:944	a systematic study	927:944	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	72	theme	human	1274:1278	arg1	IgGs					1280:1283	human IgGs	1274:1283	human IgGs	1274:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	1	73	theme	human	131:135	arg1	IgGs					156:159	human immunoglobulins G (IgGs)	131:160	human immunoglobulins G (IgGs)	131:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	5	74	theme	sequence	1064:1071	arg1	effect					1054:1059	the effect	1050:1059	the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs	1050:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	4	75	from	role	823:826	arg1	propensity					885:894	its intrinsic conformational propensity	856:894	its intrinsic conformational propensity	856:894	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	1	76	theme	G	153:153	arg1	IgGs					156:159	human immunoglobulins G (IgGs)	131:160	human immunoglobulins G (IgGs)	131:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	3	77	attach	linked	643:648	arg1	onset					667:671	the onset	663:671	the onset of inflammation	663:687	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	77	attach	linked	643:648	arg1	aging					653:657	aging	653:657	aging	653:657	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	77	attach	linked	643:648	arg1	predisposition					701:714	the predisposition	697:714	the predisposition to rheumatoid arthritis	697:738	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	77	attach	linked	643:648	arg2	levels					626:631	galactosylation levels	610:631	galactosylation levels	610:631	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	0	78	theme	molecular	85:93	arg1	dynamics					95:102	a molecular dynamics	83:102	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.	0:109	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	1	79	theme	IgGs	156:159	arg1	glycosylation					114:126	Fc glycosylation	111:126	Fc glycosylation of human immunoglobulins G (IgGs)	111:160	Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity.					
30325416	5	80	theme	N-glycoforms	1147:1158	arg1	dynamics					1094:1101	dynamics	1094:1101	dynamics	1094:1101	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	5	80	theme	N-glycoforms	1147:1158	arg1	structure					1080:1088	the structure	1076:1088	the structure	1076:1088	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	4	81	theme	relationship	789:800	arg1	framework					752:760	the framework	748:760	the framework of a structure-to-function relationship	748:800	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	7	82	from	arm	1803:1805	arg1	N-glycans					1838:1846	both isolated, and Fc-bound N-glycans	1810:1846	both isolated, and Fc-bound N-glycans	1810:1846	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	5	83	theme	larger	1119:1124	arg1	N-glycoforms					1147:1158	increasingly larger, complex biantennary N-glycoforms	1106:1158	increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs	1106:1283	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	3	84	gly	core-fucosylation	366:382	arg1	cytotoxicity					455:466	the antibody-dependent cell-mediated cytotoxicity	418:466	the antibody-dependent cell-mediated cytotoxicity function	418:475	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	84	gly	core-fucosylation	366:382	arg1	Fc-glycans					391:400	IgG Fc-glycans	387:400	IgG Fc-glycans	387:400	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	7	85	theme	glycan	1695:1700	arg1	data					1708:1711	glycan array data	1695:1711	glycan array data	1695:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	3	86	theme	IgG	387:389	arg1	Fc-glycans					391:400	IgG Fc-glycans	387:400	IgG Fc-glycans	387:400	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	3	87	theme	cell-mediated	441:453	arg1	cytotoxicity					455:466	the antibody-dependent cell-mediated cytotoxicity	418:466	the antibody-dependent cell-mediated cytotoxicity function	418:475	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	0	88	theme	isolated	36:43	arg1	N-glycans					72:80	isolated IgG Fc complex biantennary N-glycans	36:80	isolated IgG Fc complex biantennary N-glycans	36:80	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	5	89	theme	molecular	966:974	arg1	simulations					985:995	extensive molecular dynamics simulations	956:995	extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time	956:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	7	90	theme	isomers	1684:1690	arg1	recognition					1658:1668	a differential recognition	1643:1668	a differential recognition of positional isomers in glycan array data	1643:1711	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30325416	0	91	theme	Fc	49:50	arg1	N-glycans					72:80	isolated IgG Fc complex biantennary N-glycans	36:80	isolated IgG Fc complex biantennary N-glycans	36:80	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	5	92	theme	simulation	1030:1039	arg1	time					1041:1044	cumulative simulation time	1019:1044	cumulative simulation time	1019:1044	Here we report the results of a systematic study, based on extensive molecular dynamics simulations in excess of 62 μs of cumulative simulation time, on the effect of sequence on the structure and dynamics of increasingly larger, complex biantennary N-glycoforms isolated from the protein, i.e. from chitobiose to the larger N-glycan species commonly found in the Fc region of human IgGs.					
30325416	0	93	theme	biantennary	60:70	arg1	N-glycans					72:80	isolated IgG Fc complex biantennary N-glycans	36:80	isolated IgG Fc complex biantennary N-glycans	36:80	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	6	94	from	outstretched	1505:1516	arg1	equilibrium					1485:1495	its conformational equilibrium	1466:1495	its conformational equilibrium from an outstretched to a folded conformation	1466:1541	Our results show that while core fucosylation and sialylation do not affect the intrinsic dynamics of the unlinked N-glycans, galactosylation of the α(1-6) arm shifts dramatically its conformational equilibrium from an outstretched to a folded conformation.					
30325416	4	95	theme	sequence	844:851	arg1	role					823:826	the role	819:826	the role of the N-glycan sequence on its intrinsic conformational propensity	819:894	Within the framework of a structure-to-function relationship, we have studied the role of the N-glycan sequence on its intrinsic conformational propensity.					
30325416	3	96	theme	inflammation	676:687	arg1	onset					667:671	the onset	663:671	the onset of inflammation	663:687	Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis.					
30325416	2	97	theme	effector	305:312	arg1	function					314:321	the IgG effector function	297:321	the IgG effector function	297:321	Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties.					
30325416	0	98	dep	dependence	22:31	arg1	study					104:108	a molecular dynamics study	83:108	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.	0:109	Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.					
30325416	7	99	theme	isolated	1815:1822	arg1	N-glycans					1838:1846	both isolated, and Fc-bound N-glycans	1810:1846	both isolated, and Fc-bound N-glycans	1810:1846	These findings are in agreement with and can help rationalize recent experimental evidence showing a differential recognition of positional isomers in glycan array data and also the preference of sialyltransferase for the more accessible, outstretched α(1-3) arm in both isolated, and Fc-bound N-glycans.					
30335821	3	0	theme	yeast	904:908	arg1	Kex2p					893:897	Kex2p	893:897	Kex2p	893:897	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	3	0	theme	yeast	904:908	arg1	homolog					925:931	the yeast furin protease homolog	900:931	the yeast furin protease homolog	900:931	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	2	1	from	expression	525:534	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	4	2	link	N-linked	1197:1204	arg1	glycan					1206:1211	N-linked glycan	1197:1211	N-linked glycan	1197:1211	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	2	3	theme	gp140	549:553	arg1	Env					555:557	gp120 and gp140 Env	539:557	gp120 and gp140 Env from different viral strains	539:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	7	4	theme	envelope	1817:1824	arg1	protein					1826:1832	the yeast-displayed envelope protein	1797:1832	the yeast-displayed envelope protein	1797:1832	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	1	5	theme	surface	232:238	arg1	system					248:253	the yeast surface display system	222:253	the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies	222:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	4	6	theme	CD4	1060:1062	arg1	loop					1054:1057	the V3-variable loop	1038:1057	the V3-variable loop	1038:1057	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	4	6	theme	CD4	1060:1062	arg1	site					1072:1075	CD4 binding site	1060:1075	CD4 binding site	1060:1075	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	1	7	theme	Env	142:144	arg1	forms					133:137	variant forms	125:137	variant forms of Env with enhanced immunogenic properties	125:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	8	theme	viral	574:578	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	5	9	gly	N-glycosylation	1340:1354	arg1	cells					1369:1373	mammalian cells	1359:1373	mammalian cells	1359:1373	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	1	10	from	system	248:253	arg1	form					260:263	a form	258:263	a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies	258:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	1	11	theme	enhanced	151:158	arg1	properties					172:181	enhanced immunogenic properties	151:181	enhanced immunogenic properties	151:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	12	link	N-linked	696:703	arg1	glycosylation					705:717	N-linked glycosylation	696:717	N-linked glycosylation of Env	696:724	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	7	13	theme	anti-Env	1749:1756	arg1	antibodies					1758:1767	anti-Env antibodies	1749:1767	anti-Env antibodies	1749:1767	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	2	14	theme	cell	504:507	arg1	anchoring					514:522	cell wall anchoring	504:522	cell wall anchoring	504:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	3	15	gly	glycoprotein	770:781	arg1	glycoprotein					770:781	HIV envelope glycoprotein	757:781	HIV envelope glycoprotein	757:781	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	2	16	theme	Env	722:724	arg1	glycosylation					705:717	N-linked glycosylation	696:717	N-linked glycosylation of Env	696:724	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	1	17	theme	enhanced	346:353	arg1	binding					355:361	enhanced binding	346:361	enhanced binding to germline antibodies	346:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	18	theme	gp120	539:543	arg1	Env					555:557	gp120 and gp140 Env	539:557	gp120 and gp140 Env from different viral strains	539:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	19	from	effects	593:599	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	7	20	theme	unmutated	1725:1733	arg1	precursors					1735:1744	reconstructed predicted unmutated precursors	1701:1744	reconstructed predicted unmutated precursors to anti-Env antibodies	1701:1767	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	7	21	with	Consistent	1659:1668	arg1	studies					1675:1681	studies	1675:1681	studies in other systems	1675:1698	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	6	22	theme	monomeric	1543:1551	arg1	state					1553:1557	a monomeric state	1541:1557	a monomeric state	1541:1557	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	7	23	theme	reconstructed	1701:1713	arg1	precursors					1735:1744	reconstructed predicted unmutated precursors	1701:1744	reconstructed predicted unmutated precursors to anti-Env antibodies	1701:1767	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	2	24	theme	procedures	672:681	arg1	expression					525:534	expression	525:534	expression of gp120 and gp140 Env from different viral strains	525:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	24	theme	procedures	672:681	arg1	effects					593:599	the effects	589:599	the effects of introducing mutations designed to stabilize Env	589:650	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	24	theme	procedures	672:681	arg1	effects					661:667	the effects	657:667	the effects of procedures	657:681	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	24	theme	procedures	672:681	arg1	approaches					489:498	different approaches	479:498	different approaches for cell wall anchoring	479:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	5	25	theme	antibody	1223:1230	arg1	recognition					1232:1242	antibody recognition	1223:1242	antibody recognition	1223:1242	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	0	26	theme	yeast	43:47	arg1	surface					54:60	the yeast cell surface	39:60	the yeast cell surface	39:60	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	3	27	theme	cell	825:828	arg1	surface					830:836	the yeast cell surface	815:836	the yeast cell surface	815:836	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	2	28	theme	Env	456:458	arg1	protein					460:466	the yeast-displayed Env protein	436:466	the yeast-displayed Env protein	436:466	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	4	29	theme	membrane-proximal	1087:1103	arg1	regions					1105:1111	gp41 membrane-proximal regions	1082:1111	gp41 membrane-proximal regions	1082:1111	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	4	29	theme	membrane-proximal	1087:1103	arg1	antibodies					1129:1138	some antibodies	1124:1138	some antibodies whose binding is known to depend on Env conformation and N-linked glycan	1124:1211	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	3	30	theme	glycoprotein	770:781	arg1	forms					748:752	diverse forms	740:752	diverse forms of HIV envelope glycoprotein	740:781	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	1	31	theme	random	290:295	arg1	mutagenesis					297:307	random mutagenesis	290:307	random mutagenesis	290:307	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	1	32	theme	forms	133:137	arg1	development					110:120	the development	106:120	the development of variant forms of Env with enhanced immunogenic properties	106:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	3	33	theme	HIV	757:759	arg1	glycoprotein					770:781	HIV envelope glycoprotein	757:781	HIV envelope glycoprotein	757:781	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	0	34	theme	immunogen	66:74	arg1	development					76:86	immunogen development	66:86	immunogen development	66:86	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	2	35	gly	glycosylation	705:717	arg1	Env					722:724	Env	722:724	Env	722:724	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	1	36	gly	glycoprotein	206:217	arg1	glycoprotein					206:217	the glycoprotein	202:217	the glycoprotein	202:217	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	1	36	gly	glycoprotein	206:217	arg1	step					94:97	a step	92:97	a step toward the development of variant forms of Env with enhanced immunogenic properties	92:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	3	37	theme	gp140	847:851	arg1	forms					853:857	gp140 forms	847:857	gp140 forms of Env	847:864	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	6	38	theme	yeast	1589:1593	arg1	glycosylation					1595:1607	yeast glycosylation	1589:1607	yeast glycosylation in certain regions	1589:1626	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	5	39	theme	glycosylation	1283:1295	arg1	patterns					1297:1304	yeast glycosylation patterns	1277:1304	yeast glycosylation patterns	1277:1304	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	1	40	theme	display	240:246	arg1	system					248:253	the yeast surface display system	222:253	the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies	222:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	6	41	theme	yeast-displayed	1385:1399	arg1	Env					1401:1403	yeast-displayed Env	1385:1403	yeast-displayed Env	1385:1403	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	0	42	theme	envelope	19:26	arg1	protein					28:34	the HIV envelope protein	11:34	the HIV envelope protein	11:34	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	5	43	from	N-glycosylation	1340:1354	arg1	cells					1369:1373	mammalian cells	1359:1373	mammalian cells	1359:1373	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	4	44	theme	yeast-displayed	943:957	arg1	gp120					959:963	Multiple yeast-displayed gp120 and gp140 proteins	934:982	gp120	959:963	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	6	45	gly	glycosylation	1595:1607	arg1	regions					1620:1626	certain regions	1612:1626	certain regions	1612:1626	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	2	46	from	strains	580:586	arg1	Env					555:557	gp120 and gp140 Env	539:557	gp120 and gp140 Env from different viral strains	539:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	46	from	strains	580:586	arg1	expression					525:534	expression	525:534	expression of gp120 and gp140 Env from different viral strains	525:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	46	from	strains	580:586	arg1	effects					593:599	the effects	589:599	the effects of introducing mutations designed to stabilize Env	589:650	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	46	from	strains	580:586	arg1	effects					661:667	the effects	657:667	the effects of procedures	657:681	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	46	from	strains	580:586	arg1	approaches					489:498	different approaches	479:498	different approaches for cell wall anchoring	479:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	5	47	theme	mammalian	1359:1367	arg1	cells					1369:1373	mammalian cells	1359:1373	mammalian cells	1359:1373	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	1	48	with	Env	142:144	arg1	properties					172:181	enhanced immunogenic properties	151:181	enhanced immunogenic properties	151:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	3	49	theme	furin	910:914	arg1	Kex2p					893:897	Kex2p	893:897	Kex2p	893:897	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	3	49	theme	furin	910:914	arg1	homolog					925:931	the yeast furin protease homolog	900:931	the yeast furin protease homolog	900:931	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	4	50	theme	N-linked	1197:1204	arg1	glycan					1206:1211	N-linked glycan	1197:1211	N-linked glycan	1197:1211	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	6	51	from	glycosylation	1595:1607	arg1	regions					1620:1626	certain regions	1612:1626	certain regions	1612:1626	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	7	52	theme	yeast-displayed	1801:1815	arg1	protein					1826:1832	the yeast-displayed envelope protein	1797:1832	the yeast-displayed envelope protein	1797:1832	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	5	53	theme	high	1322:1325	arg1	N-glycosylation					1340:1354	high mannose-type N-glycosylation	1322:1354	high mannose-type N-glycosylation in mammalian cells	1322:1373	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	2	54	theme	Env	555:557	arg1	expression					525:534	expression	525:534	expression of gp120 and gp140 Env from different viral strains	525:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	54	theme	Env	555:557	arg1	effects					593:599	the effects	589:599	the effects of introducing mutations designed to stabilize Env	589:650	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	54	theme	Env	555:557	arg1	effects					661:667	the effects	657:667	the effects of procedures	657:681	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	54	theme	Env	555:557	arg1	approaches					489:498	different approaches	479:498	different approaches for cell wall anchoring	479:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	1	55	theme	yeast	226:230	arg1	system					248:253	the yeast surface display system	222:253	the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies	222:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	7	56	theme	little	1777:1782	arg1	affinity					1784:1791	little affinity	1777:1791	little affinity for the yeast-displayed envelope protein	1777:1832	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	4	57	theme	binding	1064:1070	arg1	loop					1054:1057	the V3-variable loop	1038:1057	the V3-variable loop	1038:1057	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	4	57	theme	binding	1064:1070	arg1	site					1072:1075	CD4 binding site	1060:1075	CD4 binding site	1060:1075	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	6	58	theme	displayed	1513:1521	arg1	protein					1523:1529	the displayed protein	1509:1529	the displayed protein	1509:1529	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	2	59	theme	N-linked	696:703	arg1	glycosylation					705:717	N-linked glycosylation	696:717	N-linked glycosylation of Env	696:724	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	60	theme	different	564:572	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	4	61	theme	V3-variable	1042:1052	arg1	loop					1054:1057	the V3-variable loop	1038:1057	the V3-variable loop	1038:1057	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	4	61	theme	V3-variable	1042:1052	arg1	site					1072:1075	CD4 binding site	1060:1075	CD4 binding site	1060:1075	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	1	62	theme	immunogenic	160:170	arg1	properties					172:181	enhanced immunogenic properties	151:181	enhanced immunogenic properties	151:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	63	theme	wall	509:512	arg1	anchoring					514:522	cell wall anchoring	504:522	cell wall anchoring	504:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	64	from	effects	661:667	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	1	65	theme	germline	366:373	arg1	antibodies					375:384	germline antibodies	366:384	germline antibodies	366:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	66	from	approaches	489:498	arg1	strains					580:586	different viral strains	564:586	different viral strains	564:586	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	0	67	theme	cell	49:52	arg1	surface					54:60	the yeast cell surface	39:60	the yeast cell surface	39:60	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	7	68	theme	other	1686:1690	arg1	systems					1692:1698	other systems	1686:1698	other systems	1686:1698	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	7	69	theme	predicted	1715:1723	arg1	precursors					1735:1744	reconstructed predicted unmutated precursors	1701:1744	reconstructed predicted unmutated precursors to anti-Env antibodies	1701:1767	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	2	70	theme	protein	460:466	arg1	immunogenicity					418:431	immunogenicity	418:431	immunogenicity	418:431	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	2	70	theme	protein	460:466	arg1	expression					403:412	expression	403:412	expression	403:412	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	7	71	from	studies	1675:1681	arg1	systems					1692:1698	other systems	1686:1698	other systems	1686:1698	Consistent with studies in other systems, reconstructed predicted unmutated precursors to anti-Env antibodies exhibit little affinity for the yeast-displayed envelope protein.					
30335821	3	72	theme	diverse	740:746	arg1	forms					748:752	diverse forms	740:752	diverse forms of HIV envelope glycoprotein	740:781	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	1	73	theme	variant	125:131	arg1	forms					133:137	variant forms	125:137	variant forms of Env with enhanced immunogenic properties	125:181	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	2	74	theme	yeast-displayed	440:454	arg1	protein					460:466	the yeast-displayed Env protein	436:466	the yeast-displayed Env protein	436:466	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	6	75	theme	certain	1612:1618	arg1	regions					1620:1626	certain regions	1612:1626	certain regions	1612:1626	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	3	76	theme	yeast	819:823	arg1	surface					830:836	the yeast cell surface	815:836	the yeast cell surface	815:836	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	2	77	theme	different	479:487	arg1	approaches					489:498	different approaches	479:498	different approaches for cell wall anchoring	479:522	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	4	78	theme	Env	1176:1178	arg1	conformation					1180:1191	Env conformation	1176:1191	Env conformation	1176:1191	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	3	79	theme	envelope	761:768	arg1	glycoprotein					770:781	HIV envelope glycoprotein	757:781	HIV envelope glycoprotein	757:781	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	5	80	theme	yeast	1277:1281	arg1	patterns					1297:1304	yeast glycosylation patterns	1277:1304	yeast glycosylation patterns	1277:1304	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	2	81	dep	expression	403:412	arg1	the					399:401	the	399:401	the	399:401	To optimize the expression and immunogenicity of the yeast-displayed Env protein, we tested different approaches for cell wall anchoring, expression of gp120 and gp140 Env from different viral strains, the effects of introducing mutations designed to stabilize Env, and the effects of procedures for altering N-linked glycosylation of Env.					
30335821	0	82	from	surface	54:60	arg1	Display					0:6	Display	0:6	Display of the HIV envelope protein at the yeast cell surface for immunogen development	0:86	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	0	83	theme	HIV	15:17	arg1	protein					28:34	the HIV envelope protein	11:34	the HIV envelope protein	11:34	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	4	84	theme	gp140	969:973	arg1	proteins					975:982	Multiple yeast-displayed gp120 and gp140 proteins	934:982	proteins	975:982	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	1	85	dep	subjected	277:285	arg1	followed					309:316	followed	309:316	followed by screening for forms with enhanced binding to germline antibodies	309:384	As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display system in a form that can be subjected to random mutagenesis followed by screening for forms with enhanced binding to germline antibodies.					
30335821	0	86	theme	protein	28:34	arg1	Display					0:6	Display	0:6	Display of the HIV envelope protein at the yeast cell surface for immunogen development	0:86	Display of the HIV envelope protein at the yeast cell surface for immunogen development.					
30335821	3	87	theme	Env	862:864	arg1	forms					853:857	gp140 forms	847:857	gp140 forms of Env	847:864	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	5	88	theme	mannose-type	1327:1338	arg1	N-glycosylation					1340:1354	high mannose-type N-glycosylation	1322:1354	high mannose-type N-glycosylation in mammalian cells	1322:1373	Based on antibody recognition and sensitivity to glycosidases, yeast glycosylation patterns partially mimic high mannose-type N-glycosylation in mammalian cells.					
30335821	4	89	theme	Multiple	934:941	arg1	gp120					959:963	Multiple yeast-displayed gp120 and gp140 proteins	934:982	gp120	959:963	Multiple yeast-displayed gp120 and gp140 proteins are capable of binding to antibodies directed against the V3-variable loop, CD4 binding site, and gp41 membrane-proximal regions, including some antibodies whose binding is known to depend on Env conformation and N-linked glycan.					
30335821	6	90	theme	quaternary	1464:1473	arg1	structure					1475:1483	quaternary structure	1464:1483	quaternary structure	1464:1483	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	6	91	theme	sensitive	1451:1459	arg1	antibodies					1440:1449	some anti-Env antibodies	1426:1449	some anti-Env antibodies sensitive to quaternary structure	1426:1483	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
30335821	3	92	theme	protease	916:923	arg1	Kex2p					893:897	Kex2p	893:897	Kex2p	893:897	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	3	92	theme	protease	916:923	arg1	homolog					925:931	the yeast furin protease homolog	900:931	the yeast furin protease homolog	900:931	We find that diverse forms of HIV envelope glycoprotein can be efficiently expressed at the yeast cell surface and that gp140 forms of Env are effectively cleaved by Kex2p, the yeast furin protease homolog.					
30335821	6	93	theme	anti-Env	1431:1438	arg1	antibodies					1440:1449	some anti-Env antibodies	1426:1449	some anti-Env antibodies sensitive to quaternary structure	1426:1483	However, yeast-displayed Env is not recognized by some anti-Env antibodies sensitive to quaternary structure, suggesting either that the displayed protein exists in a monomeric state or that for these antibodies, yeast glycosylation in certain regions hinders recognition or access.					
29603058	1	0	theme	automated	419:427	arg1	methods					429:435	improved and automated methods	406:435	improved and automated methods	406:435	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	6	1	theme	useful	1525:1530	arg1	tool					1532:1535	a useful tool	1523:1535	a useful tool for automated assessment of glycan isomer discrimination	1523:1592	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	6	1	theme	useful	1525:1530	arg1	Skyline					1493:1499	Skyline	1493:1499	Skyline	1493:1499	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	2	2	theme	released	701:708	arg1	isomers					726:732	released N- and O-glycan isomers	701:732	released N- and O-glycan isomers	701:732	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	4	3	theme	specificity	1107:1117	arg1	ions					1094:1097	at least two diagnostic fragment ions	1061:1097	at least two diagnostic fragment ions of high specificity	1061:1117	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	1	4	dep	demand	395:400	arg1	confirm					440:446	confirm	440:446	to confirm detailed structural features	437:475	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	1	4	dep	demand	395:400	arg1	discriminate					484:495	discriminate	484:495	to discriminate between structurally similar isomers	481:532	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	2	5	theme	porous	639:644	arg1	carbon-LC-ESI-MS/MS					658:676	porous graphitized carbon-LC-ESI-MS/MS	639:676	porous graphitized carbon-LC-ESI-MS/MS	639:676	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	2	6	theme	resonance	789:797	arg1	CID-MS/MS					810:818	negative mode resonance activation CID-MS/MS	775:818	negative mode resonance activation CID-MS/MS	775:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	0	7	theme	similar	205:211	arg1	structures					220:229	highly similar glycan structures	198:229	highly similar glycan structures that play diverse roles in cellular physiology	198:276	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	8	theme	detailed	448:455	arg1	features					468:475	detailed structural features	448:475	detailed structural features	448:475	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	4	9	theme	N-glycan	1198:1205	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	0	10	theme	structures	220:229	arg1	presence					186:193	the presence	182:193	the presence of highly similar glycan structures that play diverse roles in cellular physiology	182:276	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	4	11	from	position	1186:1193	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	2	12	used	used	634:637	arg2	We					631:632	We	631:632	We	631:632	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	6	13	theme	discrimination	1579:1592	arg1	assessment					1551:1560	automated assessment	1541:1560	automated assessment of glycan isomer discrimination	1541:1592	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	0	14	theme	protein	138:144	arg1	glycosylation					146:158	cellular protein glycosylation	129:158	cellular protein glycosylation	129:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	7	15	theme	glycan	1729:1734	arg1	isomers					1736:1742	other challenging glycan isomers	1711:1742	other challenging glycan isomers	1711:1742	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	4	16	theme	O-glycan	1238:1245	arg1	structures					1247:1256	O-glycan structures	1238:1256	O-glycan structures	1238:1256	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	7	17	theme	other	1711:1715	arg1	isomers					1736:1742	other challenging glycan isomers	1711:1742	other challenging glycan isomers	1711:1742	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	1	18	from	bottleneck	560:569	arg1	analysis					578:585	the analysis	574:585	the analysis of data generated by glycomics experiments	574:628	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	1	19	theme	improved	406:413	arg1	methods					429:435	improved and automated methods	406:435	improved and automated methods	406:435	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	3	20	theme	similar	838:844	arg1	spectra					855:861	similar fragment spectra	838:861	similar fragment spectra	838:861	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	4	21	theme	Skyline	1043:1049	arg1	software					1051:1058	the Skyline software	1039:1058	the Skyline software	1039:1058	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	2	22	theme	mammalian	739:747	arg1	glycoproteins					755:767	mammalian model glycoproteins	739:767	mammalian model glycoproteins using negative mode resonance activation CID-MS/MS	739:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	3	23	theme	glycan	884:889	arg1	isomers					891:897	closely related glycan isomers	868:897	closely related glycan isomers that differ only in arm position and sialyl linkage	868:949	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	0	24	theme	Product	72:78	arg1	Ions					80:83	Diagnostic Product Ions	61:83	Diagnostic Product Ions	61:83	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	25	theme	glycosidic	337:346	arg1	bond					348:351	a single glycosidic bond	328:351	a single glycosidic bond	328:351	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	0	26	theme	diverse	241:247	arg1	roles					249:253	diverse roles	241:253	diverse roles	241:253	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	3	27	theme	arm	919:921	arg1	position					923:930	arm position	919:930	arm position	919:930	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	3	28	theme	product	952:958	arg1	ions					969:972	product fragment ions	952:972	product fragment ions for discrimination between these features	952:1014	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	2	29	theme	graphitized	646:656	arg1	carbon-LC-ESI-MS/MS					658:676	porous graphitized carbon-LC-ESI-MS/MS	639:676	porous graphitized carbon-LC-ESI-MS/MS	639:676	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	1	30	theme	significant	548:558	arg1	bottleneck					560:569	a significant bottleneck	546:569	a significant bottleneck in the analysis of data generated by glycomics experiments	546:628	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	0	31	theme	Ion	97:99	arg1	Profiling					119:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	32	theme	glycan	367:372	arg1	function					374:381	glycan function	367:381	glycan function	367:381	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	4	33	theme	diagnostic	1074:1083	arg1	ions					1094:1097	at least two diagnostic fragment ions	1061:1097	at least two diagnostic fragment ions of high specificity	1061:1117	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	4	34	theme	existing	1273:1280	arg1	ions					1304:1307	existing structural diagnostic ions	1273:1307	existing structural diagnostic ions	1273:1307	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	1	35	theme	glycomics	608:616	arg1	experiments					618:628	glycomics experiments	608:628	glycomics experiments	608:628	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	2	36	gly	glycoproteins	755:767	arg1	glycoproteins					755:767	mammalian model glycoproteins	739:767	mammalian model glycoproteins using negative mode resonance activation CID-MS/MS	739:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	4	37	theme	diagnostic	1293:1302	arg1	ions					1304:1307	existing structural diagnostic ions	1273:1307	existing structural diagnostic ions	1273:1307	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	0	38	theme	Isomers	18:24	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation	0:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	4	39	theme	position	1186:1193	arg1	sialylation					1223:1233	sialylation	1223:1233	sialylation	1223:1233	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	4	39	theme	position	1186:1193	arg1	discrimination					1148:1161	automated discrimination	1138:1161	automated discrimination of sialylation and arm position in N-glycan structures	1138:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	1	40	theme	data	590:593	arg1	analysis					578:585	the analysis	574:585	the analysis of data generated by glycomics experiments	574:628	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	0	41	theme	Released	29:36	arg1	N-					38:39	N-	38:39	N-	38:39	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	42	theme	exact	306:310	arg1	type					320:323	the exact linkage type	302:323	the exact linkage type	302:323	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	7	43	theme	platform-independent	1600:1619	arg1	workflow					1621:1628	This platform-independent workflow	1595:1628	This platform-independent workflow	1595:1628	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	4	44	gly	sialylation	1223:1233	arg1	sialylation					1166:1176	sialylation	1166:1176	sialylation	1166:1176	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	4	44	gly	sialylation	1223:1233	arg1	position					1186:1193	arm position	1182:1193	arm position	1182:1193	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	5	45	theme	cell	1479:1482	arg1	lysates					1484:1490	cell lysates	1479:1490	cell lysates	1479:1490	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	4	46	from	sialylation	1223:1233	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	5	47	dep	linear	1393:1398	arg1	spectrometers					1421:1433	spectrometers	1421:1433	spectrometers	1421:1433	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	6	48	theme	automated	1541:1549	arg1	assessment					1551:1560	automated assessment	1541:1560	automated assessment of glycan isomer discrimination	1541:1592	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	5	49	theme	diagnostic	1316:1325	arg1	ions					1327:1330	These diagnostic ions	1310:1330	These diagnostic ions	1310:1330	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	4	50	theme	high	1102:1105	arg1	specificity					1107:1117	high specificity	1102:1117	high specificity	1102:1117	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	5	51	theme	complex	1450:1456	arg1	mixtures					1465:1472	complex glycan mixtures	1450:1472	complex glycan mixtures	1450:1472	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	8	52	theme	Graphical	1745:1753	arg1	Abstract					1755:1762	Graphical Abstract	1745:1762	Graphical Abstract	1745:1762	Graphical Abstract ᅟ.					
29603058	2	53	theme	activation	799:808	arg1	CID-MS/MS					810:818	negative mode resonance activation CID-MS/MS	775:818	negative mode resonance activation CID-MS/MS	775:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	2	54	theme	negative	775:782	arg1	CID-MS/MS					810:818	negative mode resonance activation CID-MS/MS	775:818	negative mode resonance activation CID-MS/MS	775:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	0	55	theme	glycan	213:218	arg1	structures					220:229	highly similar glycan structures	198:229	highly similar glycan structures that play diverse roles in cellular physiology	198:276	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	4	56	from	structures	1207:1216	arg1	sialylation					1223:1233	sialylation	1223:1233	sialylation	1223:1233	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	4	56	from	structures	1207:1216	arg1	discrimination					1148:1161	automated discrimination	1138:1161	automated discrimination of sialylation and arm position in N-glycan structures	1138:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	2	57	theme	mode	784:787	arg1	CID-MS/MS					810:818	negative mode resonance activation CID-MS/MS	775:818	negative mode resonance activation CID-MS/MS	775:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	1	58	theme	structural	457:466	arg1	features					468:475	detailed structural features	448:475	detailed structural features	448:475	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	4	59	theme	sialylation	1166:1176	arg1	sialylation					1223:1233	sialylation	1223:1233	sialylation	1223:1233	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	4	59	theme	sialylation	1166:1176	arg1	discrimination					1148:1161	automated discrimination	1138:1161	automated discrimination of sialylation and arm position in N-glycan structures	1138:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	0	60	theme	cellular	129:136	arg1	glycosylation					146:158	cellular protein glycosylation	129:158	cellular protein glycosylation	129:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	7	61	theme	isomers	1736:1742	arg1	characterization					1674:1689	characterization	1674:1689	characterization	1674:1689	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	7	61	theme	isomers	1736:1742	arg1	quantitation					1695:1706	quantitation	1695:1706	quantitation	1695:1706	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	4	62	theme	arm	1182:1184	arg1	position					1186:1193	arm position	1182:1193	arm position	1182:1193	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	7	63	dep	characterization	1674:1689	arg1	the					1670:1672	the	1670:1672	the	1670:1672	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	6	64	theme	isomer	1572:1577	arg1	discrimination					1579:1592	glycan isomer discrimination	1565:1592	glycan isomer discrimination	1565:1592	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	4	65	from	sialylation	1166:1176	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	7	66	theme	challenging	1717:1727	arg1	isomers					1736:1742	other challenging glycan isomers	1711:1742	other challenging glycan isomers	1711:1742	This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.					
29603058	4	67	from	discrimination	1148:1161	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	2	68	theme	O-glycan	717:724	arg1	isomers					726:732	released N- and O-glycan isomers	701:732	released N- and O-glycan isomers	701:732	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	5	69	theme	isomer	1360:1365	arg1	discrimination					1367:1380	isomer discrimination	1360:1380	isomer discrimination using both linear and 3D ion trap mass spectrometers	1360:1433	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	0	70	theme	Diagnostic	61:70	arg1	Ions					80:83	Diagnostic Product Ions	61:83	Diagnostic Product Ions	61:83	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	2	71	theme	model	749:753	arg1	glycoproteins					755:767	mammalian model glycoproteins	739:767	mammalian model glycoproteins using negative mode resonance activation CID-MS/MS	739:818	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	3	72	theme	fragment	846:853	arg1	spectra					855:861	similar fragment spectra	838:861	similar fragment spectra	838:861	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	1	73	theme	single	330:335	arg1	bond					348:351	a single glycosidic bond	328:351	a single glycosidic bond	328:351	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	3	74	theme	sialyl	936:941	arg1	linkage					943:949	sialyl linkage	936:949	sialyl linkage	936:949	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	0	75	theme	Negative	88:95	arg1	Profiling					119:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	76	theme	bond	348:351	arg1	type					320:323	the exact linkage type	302:323	the exact linkage type	302:323	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	1	76	theme	bond	348:351	arg1	anomericity					286:296	the anomericity	282:296	the anomericity	282:296	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	5	77	theme	3D	1404:1405	arg1	mass					1416:1419	3D ion trap mass	1404:1419	3D ion trap mass	1404:1419	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	3	78	theme	related	876:882	arg1	isomers					891:897	closely related glycan isomers	868:897	closely related glycan isomers that differ only in arm position and sialyl linkage	868:949	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	0	79	theme	PGC-LC-ESI-MS/MS.	101:117	arg1	Profiling					119:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Negative Ion PGC-LC-ESI-MS/MS. Profiling	88:127	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	4	80	gly	sialylation	1166:1176	arg1	structures					1207:1216	N-glycan structures	1198:1216	N-glycan structures	1198:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	3	81	theme	fragment	960:967	arg1	ions					969:972	product fragment ions	952:972	product fragment ions for discrimination between these features	952:1014	By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered.					
29603058	4	82	theme	structural	1282:1291	arg1	ions					1304:1307	existing structural diagnostic ions	1273:1307	existing structural diagnostic ions	1273:1307	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	5	83	theme	trap	1411:1414	arg1	mass					1416:1419	3D ion trap mass	1404:1419	3D ion trap mass	1404:1419	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	0	84	theme	cellular	258:265	arg1	physiology					267:276	cellular physiology	258:276	cellular physiology	258:276	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	4	85	theme	fragment	1085:1092	arg1	ions					1094:1097	at least two diagnostic fragment ions	1061:1097	at least two diagnostic fragment ions of high specificity	1061:1117	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
29603058	0	86	theme	N-	38:39	arg1	Isomers					18:24	Isomers	18:24	Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation	18:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	5	87	theme	ion	1407:1409	arg1	mass					1416:1419	3D ion trap mass	1404:1419	3D ion trap mass	1404:1419	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	5	88	theme	glycan	1458:1463	arg1	mixtures					1465:1472	complex glycan mixtures	1450:1472	complex glycan mixtures	1450:1472	These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates.					
29603058	0	89	theme	O-Glycans	45:53	arg1	Isomers					18:24	Isomers	18:24	Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation	18:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	1	90	theme	linkage	312:318	arg1	type					320:323	the exact linkage type	302:323	the exact linkage type	302:323	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	0	91	dep	N-	38:39	arg1	glycosylation					146:158	cellular protein glycosylation	129:158	cellular protein glycosylation	129:158	Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS. Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology.					
29603058	6	92	theme	glycan	1565:1570	arg1	discrimination					1579:1592	glycan isomer discrimination	1565:1592	glycan isomer discrimination	1565:1592	Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination.					
29603058	2	93	theme	N-	710:711	arg1	isomers					726:732	released N- and O-glycan isomers	701:732	released N- and O-glycan isomers	701:732	We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS.					
29603058	1	94	theme	similar	518:524	arg1	isomers					526:532	structurally similar isomers	505:532	structurally similar isomers	505:532	As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments.					
29603058	4	95	theme	automated	1138:1146	arg1	discrimination					1148:1161	automated discrimination	1138:1161	automated discrimination of sialylation and arm position in N-glycan structures	1138:1216	Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions.					
30959459	0	0	theme	ST6Gal-I	94:101	arg1	activation					52:61	catalytic activation	42:61	catalytic activation	42:61	A Golgi-associated redox switch regulates catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	6	1	theme	O-linked	1402:1409	arg1	glycans					1411:1417	O-linked glycans	1402:1417	O-linked glycans in hypoxic cells	1402:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	6	2	theme	modifying	1293:1301	arg1	sialyltransferase					1303:1319	this O-glycan and glycolipid modifying sialyltransferase	1264:1319	sialyltransferase	1303:1319	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	1	3	theme	proteins	167:174	arg1	modification					142:153	a common modification	133:153	a common modification of cellular proteins and lipids	133:185	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	3	theme	proteins	167:174	arg1	Glycosylation					118:130	Glycosylation	118:130	Glycosylation	118:130	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	2	4	theme	glycans	478:484	arg1	sialylation					447:457	terminal sialylation	438:457	terminal sialylation of N- and O-linked glycans	438:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	5	5	theme	ST6Gal-I	1122:1129	arg1	inactivation					1102:1113	inactivation	1102:1113	inactivation of the ST6Gal-I	1102:1129	Cells expressing the inactive mutant, but not those expressing the wild type ST6Gal-I, were able to proliferate and migrate normally, supporting the view that inactivation of the ST6Gal-I help cells to adapt to hypoxic environment.					
30959459	6	6	theme	glycolipid	1282:1291	arg1	sialyltransferase					1303:1319	this O-glycan and glycolipid modifying sialyltransferase	1264:1319	sialyltransferase	1303:1319	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	6	7	link	O-linked	1402:1409	arg1	glycans					1411:1417	O-linked glycans	1402:1417	O-linked glycans in hypoxic cells	1402:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	7	8	theme	catalytic	1558:1566	arg1	activation					1568:1577	catalytic activation	1558:1577	catalytic activation	1558:1577	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	3	9	from	bonds	635:639	arg1	ability					715:721	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	9	from	bonds	635:639	arg1	domain					658:663	the catalytic domain	644:663	the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	644:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	9	from	bonds	635:639	arg1	α-2,6-sialyltransferase					672:694	the α-2,6-sialyltransferase	668:694	the α-2,6-sialyltransferase (ST6Gal-I)	668:705	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	10	theme	disulfide	625:633	arg1	bonds					635:639	two surface-exposed disulfide bonds	605:639	two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	605:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	6	11	theme	disulfide	1214:1222	arg1	bonds					1224:1228	similar disulfide bonds	1206:1228	similar disulfide bonds	1206:1228	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	5	12	theme	inactive	964:971	arg1	mutant					973:978	the inactive mutant	960:978	the inactive mutant	960:978	Cells expressing the inactive mutant, but not those expressing the wild type ST6Gal-I, were able to proliferate and migrate normally, supporting the view that inactivation of the ST6Gal-I help cells to adapt to hypoxic environment.					
30959459	6	13	theme	attenuated	1376:1385	arg1	sialylation					1387:1397	attenuated sialylation	1376:1397	attenuated sialylation of O-linked glycans in hypoxic cells	1376:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	7	14	theme	unknown	1486:1492	arg1	responsible					1538:1548	responsible	1538:1548	responsible	1538:1548	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	7	14	theme	unknown	1486:1492	arg1	switch					1500:1505	a previously unknown redox switch	1473:1505	a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I	1473:1631	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	6	15	theme	similar	1206:1212	arg1	bonds					1224:1228	similar disulfide bonds	1206:1228	similar disulfide bonds	1206:1228	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	1	16	gly	Glycosylation	118:130	arg1	diseases					208:215	diseases	208:215	diseases	208:215	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	16	gly	Glycosylation	118:130	arg1	states					240:245	pathophysiological states	221:245	pathophysiological states	221:245	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	16	gly	Glycosylation	118:130	arg1	hypoxia					255:261	hypoxia	255:261	hypoxia	255:261	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	4	17	from	residues	859:866	arg1	ST6Gal-I					871:878	ST6Gal-I	871:878	ST6Gal-I	871:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	4	18	theme	cysteine	850:857	arg1	residues					859:866	selected cysteine residues	841:866	selected cysteine residues in ST6Gal-I	841:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	1	19	theme	lipids	180:185	arg1	modification					142:153	a common modification	133:153	a common modification of cellular proteins and lipids	133:185	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	19	theme	lipids	180:185	arg1	Glycosylation					118:130	Glycosylation	118:130	Glycosylation	118:130	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	2	20	theme	independent	496:506	arg1	manner					508:513	a HIF- independent manner	489:513	a HIF- independent manner	489:513	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	6	21	from	sialylation	1387:1397	arg1	cells					1430:1434	hypoxic cells	1422:1434	hypoxic cells	1422:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	2	22	theme	O-linked	469:476	arg1	glycans					478:484	N- and O-linked glycans	462:484	N- and O-linked glycans	462:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	3	23	theme	complex	807:813	arg1	N-glycans					815:823	complex N-glycans	807:823	complex N-glycans	807:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	6	24	from	glycans	1411:1417	arg1	cells					1430:1434	hypoxic cells	1422:1434	hypoxic cells	1422:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	0	25	theme	Golgi-associated	2:17	arg1	switch					25:30	A Golgi-associated redox switch	0:30	A Golgi-associated redox switch	0:30	A Golgi-associated redox switch regulates catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	3	26	theme	ability	715:721	arg1	ability					715:721	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	26	theme	ability	715:721	arg1	domain					658:663	the catalytic domain	644:663	the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	644:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	26	theme	ability	715:721	arg1	α-2,6-sialyltransferase					672:694	the α-2,6-sialyltransferase	668:694	the α-2,6-sialyltransferase (ST6Gal-I)	668:705	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	5	27	theme	hypoxic	1154:1160	arg1	environment					1162:1172	hypoxic environment	1154:1172	hypoxic environment	1154:1172	Cells expressing the inactive mutant, but not those expressing the wild type ST6Gal-I, were able to proliferate and migrate normally, supporting the view that inactivation of the ST6Gal-I help cells to adapt to hypoxic environment.					
30959459	3	28	theme	state	565:569	arg1	change					571:576	This redox state change	554:576	This redox state change	554:576	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	7	29	theme	redox	1494:1498	arg1	responsible					1538:1548	responsible	1538:1548	responsible	1538:1548	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	7	29	theme	redox	1494:1498	arg1	switch					1500:1505	a previously unknown redox switch	1473:1505	a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I	1473:1631	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	2	30	theme	redox	388:392	arg1	screens					394:400	redox screens	388:400	redox screens	388:400	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	2	31	theme	terminal	438:445	arg1	sialylation					447:457	terminal sialylation	438:457	terminal sialylation of N- and O-linked glycans	438:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	1	32	theme	underlying	272:281	arg1	causes					293:298	the underlying molecular causes	268:298	the underlying molecular causes	268:298	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	4	33	theme	residues	859:866	arg1	Mutagenesis					826:836	Mutagenesis	826:836	Mutagenesis of selected cysteine residues in ST6Gal-I	826:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	2	34	link	O-linked	469:476	arg1	glycans					478:484	N- and O-linked glycans	462:484	N- and O-linked glycans	462:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	1	35	theme	molecular	283:291	arg1	causes					293:298	the underlying molecular causes	268:298	the underlying molecular causes	268:298	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	0	36	theme	redox	19:23	arg1	switch					25:30	A Golgi-associated redox switch	0:30	A Golgi-associated redox switch	0:30	A Golgi-associated redox switch regulates catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	7	37	theme	Golgi	1514:1518	arg1	apparatus					1520:1528	the Golgi apparatus	1510:1528	the Golgi apparatus	1510:1528	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	6	38	theme	Structure	1175:1183	arg1	comparisons					1185:1195	Structure comparisons	1175:1195	Structure comparisons	1175:1195	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	3	39	theme	redox	559:563	arg1	change					571:576	This redox state change	554:576	This redox state change	554:576	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	0	40	theme	catalytic	42:50	arg1	activation					52:61	catalytic activation	42:61	catalytic activation	42:61	A Golgi-associated redox switch regulates catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	2	41	theme	oxidative	533:541	arg1	potential					543:551	Golgi oxidative potential	527:551	Golgi oxidative potential	527:551	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	3	42	theme	catalytic	648:656	arg1	ability					715:721	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	42	theme	catalytic	648:656	arg1	domain					658:663	the catalytic domain	644:663	the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	644:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	42	theme	catalytic	648:656	arg1	α-2,6-sialyltransferase					672:694	the α-2,6-sialyltransferase	668:694	the α-2,6-sialyltransferase (ST6Gal-I)	668:705	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	5	43	theme	type	1015:1018	arg1	ST6Gal-I					1020:1027	the wild type ST6Gal-I	1006:1027	the wild type ST6Gal-I	1006:1027	Cells expressing the inactive mutant, but not those expressing the wild type ST6Gal-I, were able to proliferate and migrate normally, supporting the view that inactivation of the ST6Gal-I help cells to adapt to hypoxic environment.					
30959459	3	44	from	loss	597:600	arg1	ability					715:721	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	44	from	loss	597:600	arg1	domain					658:663	the catalytic domain	644:663	the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	644:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	44	from	loss	597:600	arg1	α-2,6-sialyltransferase					672:694	the α-2,6-sialyltransferase	668:694	the α-2,6-sialyltransferase (ST6Gal-I)	668:705	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	7	45	from	switch	1500:1505	arg1	apparatus					1520:1528	the Golgi apparatus	1510:1528	the Golgi apparatus	1510:1528	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	2	46	theme	Golgi	527:531	arg1	potential					543:551	Golgi oxidative potential	527:551	Golgi oxidative potential	527:551	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	3	47	theme	bonds	635:639	arg1	loss					597:600	loss	597:600	loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	597:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	6	48	gly	sialylation	1387:1397	arg1	glycans					1411:1417	O-linked glycans	1402:1417	O-linked glycans in hypoxic cells	1402:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	2	49	theme	N-	462:463	arg1	glycans					478:484	N- and O-linked glycans	462:484	N- and O-linked glycans	462:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	4	50	from	Mutagenesis	826:836	arg1	ST6Gal-I					871:878	ST6Gal-I	871:878	ST6Gal-I	871:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	2	51	theme	glycan	357:362	arg1	profiling					364:372	lectin microarray glycan profiling	339:372	lectin microarray glycan profiling	339:372	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	4	52	theme	inactive	933:940	arg1	enzyme					926:931	the enzyme inactive	922:940	the enzyme inactive	922:940	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	6	53	from	cells	1430:1434	arg1	sialylation					1387:1397	attenuated sialylation	1376:1397	attenuated sialylation of O-linked glycans in hypoxic cells	1376:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	4	54	theme	selected	841:848	arg1	residues					859:866	selected cysteine residues	841:866	selected cysteine residues in ST6Gal-I	841:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	2	55	theme	microarray	346:355	arg1	profiling					364:372	lectin microarray glycan profiling	339:372	lectin microarray glycan profiling	339:372	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	5	56	theme	wild	1010:1013	arg1	ST6Gal-I					1020:1027	the wild type ST6Gal-I	1006:1027	the wild type ST6Gal-I	1006:1027	Cells expressing the inactive mutant, but not those expressing the wild type ST6Gal-I, were able to proliferate and migrate normally, supporting the view that inactivation of the ST6Gal-I help cells to adapt to hypoxic environment.					
30959459	7	57	theme	ST6Gal-I	1610:1617	arg1	activation					1568:1577	catalytic activation	1558:1577	catalytic activation	1558:1577	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	3	58	theme	α-2,6-sialyltransferase	672:694	arg1	ability					715:721	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	711:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	58	theme	α-2,6-sialyltransferase	672:694	arg1	domain					658:663	the catalytic domain	644:663	the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	644:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	58	theme	α-2,6-sialyltransferase	672:694	arg1	α-2,6-sialyltransferase					672:694	the α-2,6-sialyltransferase	668:694	the α-2,6-sialyltransferase (ST6Gal-I)	668:705	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	3	59	theme	surface-exposed	609:623	arg1	bonds					635:639	two surface-exposed disulfide bonds	605:639	two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	605:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	1	60	theme	common	135:140	arg1	modification					142:153	a common modification	133:153	a common modification of cellular proteins and lipids	133:185	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	60	theme	common	135:140	arg1	Glycosylation					118:130	Glycosylation	118:130	Glycosylation	118:130	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	61	theme	pathophysiological	221:238	arg1	states					240:245	pathophysiological states	221:245	pathophysiological states	221:245	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	1	61	theme	pathophysiological	221:238	arg1	hypoxia					255:261	hypoxia	255:261	hypoxia	255:261	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	2	62	gly	sialylation	447:457	arg1	glycans					478:484	N- and O-linked glycans	462:484	N- and O-linked glycans	462:484	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	7	63	with	ST6Gal-I	1610:1617	arg1	B4GalT-I					1624:1631	B4GalT-I	1624:1631	B4GalT-I	1624:1631	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	1	64	theme	cellular	158:165	arg1	proteins					167:174	cellular proteins	158:174	cellular proteins	158:174	Glycosylation, a common modification of cellular proteins and lipids, is often altered in diseases and pathophysiological states such as hypoxia, yet the underlying molecular causes remain poorly understood.					
30959459	2	65	theme	lectin	339:344	arg1	profiling					364:372	lectin microarray glycan profiling	339:372	lectin microarray glycan profiling	339:372	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	3	66	from	domain	658:663	arg1	loss					597:600	loss	597:600	loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans	597:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	4	67	from	ST6Gal-I	871:878	arg1	Mutagenesis					826:836	Mutagenesis	826:836	Mutagenesis of selected cysteine residues in ST6Gal-I	826:878	Mutagenesis of selected cysteine residues in ST6Gal-I mimicked these effects, and also rendered the enzyme inactive.					
30959459	0	68	with	activation	52:61	arg1	B4GalT-I					108:115	B4GalT-I	108:115	B4GalT-I	108:115	A Golgi-associated redox switch regulates catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
30959459	6	69	theme	hypoxic	1422:1428	arg1	cells					1430:1434	hypoxic cells	1422:1434	hypoxic cells	1422:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	3	70	theme	preceding	784:792	arg1	galactose					794:802	the preceding galactose	780:802	the preceding galactose to complex N-glycans	780:823	This redox state change was accompanied by loss of two surface-exposed disulfide bonds in the catalytic domain of the α-2,6-sialyltransferase (ST6Gal-I) and its ability to functionally interact with B4GalT-I, an enzyme adding the preceding galactose to complex N-glycans.					
30959459	6	71	theme	glycans	1411:1417	arg1	sialylation					1387:1397	attenuated sialylation	1376:1397	attenuated sialylation of O-linked glycans in hypoxic cells	1376:1434	Structure comparisons revealed similar disulfide bonds also in ST3Gal-I, suggesting that this O-glycan and glycolipid modifying sialyltransferase is also sensitive to hypoxia and thereby contribute to attenuated sialylation of O-linked glycans in hypoxic cells.					
30959459	2	72	gly	inhibits	429:436	arg1	manner					508:513	a HIF- independent manner	489:513	a HIF- independent manner	489:513	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	2	73	theme	Golgi	375:379	arg1	pH					381:382	Golgi pH	375:382	Golgi pH	375:382	By utilizing lectin microarray glycan profiling, Golgi pH and redox screens, we show here that hypoxia inhibits terminal sialylation of N- and O-linked glycans in a HIF- independent manner by lowering Golgi oxidative potential.					
30959459	7	74	dep	activation	1568:1577	arg1	the					1554:1556	the	1554:1556	the	1554:1556	Collectively, these findings unveil a previously unknown redox switch in the Golgi apparatus that is responsible for the catalytic activation and cooperative functioning of ST6Gal-I with B4GalT-I.					
31467031	4	0	theme	affinity	1000:1007	arg1	binding					1017:1023	high affinity IgG1 Fc binding	995:1023	high affinity IgG1 Fc binding	995:1023	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	5	1	theme	intermediate	1144:1155	arg1	processing					1157:1166	intermediate processing	1144:1166	intermediate processing	1144:1166	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	6	2	theme	NK	1305:1306	arg1	CD16a					1313:1317	NK cell CD16a	1305:1317	NK cell CD16a	1305:1317	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	3	theme	cell	1308:1311	arg1	CD16a					1313:1317	NK cell CD16a	1305:1317	NK cell CD16a	1305:1317	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	3	4	theme	ligand	834:839	arg1	variability					780:790	the compositional variability	762:790	the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1	762:845	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	0	5	theme	Cell	70:73	arg1	Surface					75:81	the Cell Surface	66:81	the Cell Surface	66:81	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	5	6	dep	modified	1196:1203	arg1	complex-type					1206:1217	complex-type	1206:1217	complex-type	1206:1217	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	2	7	dep	show	456:459	arg1	known					520:524	known	520:524	is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood	517:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	1	8	theme	diverse	186:192	arg1	properties					205:214	diverse functional properties	186:214	diverse functional properties	186:214	Post-translational modification confers diverse functional properties to immune system proteins.					
31467031	6	9	theme	±	1376:1376	arg1	%					1382:1382	43.2 ± 11.0%	1371:1382	43.2 ± 11.0%	1371:1382	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	9	theme	±	1376:1376	arg1	fucosylation					1357:1368	antibody fucosylation	1348:1368	antibody fucosylation (43.2 ± 11.0%)	1348:1383	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	2	10	link	-linked	436:442	arg1	glycan					444:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	11	theme	ligands	589:595	arg1	composition					536:546	the composition	532:546	the composition of cell surface receptors or their bound ligands in situ	532:603	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	12	theme	circulating	637:647	arg1	blood					649:653	the circulating blood	633:653	the circulating blood	633:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	3	13	theme	primary	668:674	arg1	cells					679:683	primary NK cells	668:683	primary NK cells	668:683	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	2	14	theme	glycan	444:449	arg1	modification					374:385	a fucose modification	365:385	a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood	365:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	3	15	theme	bound	828:832	arg1	IgG1					842:845	IgG1	842:845	IgG1	842:845	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	3	15	theme	bound	828:832	arg1	ligand					834:839	its bound ligand	824:839	its bound ligand	824:839	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	6	16	theme	IgG1	1284:1287	arg1	ligand					1289:1294	the IgG1 ligand	1280:1294	the IgG1 ligand bound to NK cell CD16a	1280:1317	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	7	17	with	CD16a	1457:1461	arg1	patterns					1488:1495	unique modification patterns	1468:1495	unique modification patterns	1468:1495	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	1	18	theme	functional	194:203	arg1	properties					205:214	diverse functional properties	186:214	diverse functional properties	186:214	Post-translational modification confers diverse functional properties to immune system proteins.					
31467031	0	19	theme	CD16a	95:99	arg1	Glycoforms					101:110	CD16a Glycoforms	95:110	CD16a Glycoforms with Donor-dependent Variability	95:143	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	5	20	theme	Hybrid-type	1117:1127	arg1	N-glycans					1129:1137	Hybrid-type N-glycans	1117:1137	Hybrid-type N-glycans with intermediate processing	1117:1166	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	6	21	theme	same	1407:1410	arg1	%					1430:1430	89.7 ± 3.9%	1420:1430	89.7 ± 3.9%	1420:1430	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	21	theme	same	1407:1410	arg1	donors					1412:1417	the same donors	1403:1417	the same donors (89.7 ± 3.9%)	1403:1431	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	0	22	with	Glycoforms	101:110	arg1	Variability					133:143	Donor-dependent Variability	117:143	Donor-dependent Variability	117:143	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	6	23	theme	antibody	1348:1355	arg1	%					1382:1382	43.2 ± 11.0%	1371:1382	43.2 ± 11.0%	1371:1382	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	23	theme	antibody	1348:1355	arg1	fucosylation					1357:1368	antibody fucosylation	1348:1368	antibody fucosylation (43.2 ± 11.0%)	1348:1383	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	0	24	theme	Human	8:12	arg1	Cells					29:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	4	25	theme	high	995:998	arg1	binding					1017:1023	high affinity IgG1 Fc binding	995:1023	high affinity IgG1 Fc binding	995:1023	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	2	26	theme	low	616:618	arg1	abundance					620:628	low abundance	616:628	low abundance in the circulating blood	616:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	6	27	from	decrease	1336:1343	arg1	%					1382:1382	43.2 ± 11.0%	1371:1382	43.2 ± 11.0%	1371:1382	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	27	from	decrease	1336:1343	arg1	fucosylation					1357:1368	antibody fucosylation	1348:1368	antibody fucosylation (43.2 ± 11.0%)	1348:1383	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	2	28	theme	including	341:349	arg1	forms					351:355	disease including forms	333:355	disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood	333:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	0	29	theme	Primary	0:6	arg1	Cells					29:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	5	30	theme	modified	1196:1203	arg1	N-glycans					1219:1227	highly modified, complex-type N-glycans	1189:1227	highly modified, complex-type N-glycans decorated N38 and N74 from all donors	1189:1265	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	3	31	dep	plasma	718:723	arg1	donation					737:744	donation	737:744	donation	737:744	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	7	32	theme	cell	1568:1571	arg1	surface					1573:1579	the cell surface	1564:1579	the cell surface	1564:1579	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	2	33	theme	disease	333:339	arg1	forms					351:355	disease including forms	333:355	disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood	333:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	1	34	theme	immune	219:224	arg1	proteins					233:240	immune system proteins	219:240	immune system proteins	219:240	Post-translational modification confers diverse functional properties to immune system proteins.					
31467031	0	35	theme	Killer	22:27	arg1	Cells					29:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	0	36	theme	Donor-dependent	117:131	arg1	Variability					133:143	Donor-dependent Variability	117:143	Donor-dependent Variability	117:143	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	1	37	theme	system	226:231	arg1	proteins					233:240	immune system proteins	219:240	immune system proteins	219:240	Post-translational modification confers diverse functional properties to immune system proteins.					
31467031	0	38	theme	Natural	14:20	arg1	Cells					29:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells	0:33	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	2	39	theme	cell	551:554	arg1	receptors					564:572	cell surface receptors	551:572	cell surface receptors	551:572	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	0	40	from	Surface	75:81	arg1	IgG1					58:61	Proinflammatory IgG1	42:61	Proinflammatory IgG1 at the Cell Surface	42:81	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	2	41	theme	crystallizable	394:407	arg1	glycan					444:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	4	42	theme	Fc	1014:1015	arg1	binding					1017:1023	high affinity IgG1 Fc binding	995:1023	high affinity IgG1 Fc binding	995:1023	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	4	43	theme	oligomannose-type	939:955	arg1	N-glycans					957:965	only oligomannose-type N-glycans	934:965	only oligomannose-type N-glycans	934:965	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	6	44	from	donors	1412:1417	arg1	serum					1392:1396	serum	1392:1396	serum from the same donors (89.7 ± 3.9%)	1392:1431	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	2	45	theme	proteins	268:275	arg1	composition					247:257	The composition	243:257	The composition of serum proteins such as immunoglobulin G (IgG)	243:306	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	7	46	theme	NK	1440:1441	arg1	cells					1443:1447	NK cells	1440:1447	NK cells	1440:1447	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	4	47	theme	IgG1	1009:1012	arg1	binding					1017:1023	high affinity IgG1 Fc binding	995:1023	high affinity IgG1 Fc binding	995:1023	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	4	48	theme	only	934:937	arg1	N-glycans					957:965	only oligomannose-type N-glycans	934:965	only oligomannose-type N-glycans	934:965	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	7	49	theme	modification	1475:1486	arg1	patterns					1488:1495	unique modification patterns	1468:1495	unique modification patterns	1468:1495	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	2	50	theme	serum	262:266	arg1	proteins					268:275	serum proteins	262:275	serum proteins such as immunoglobulin G (IgG)	262:306	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	50	theme	serum	262:266	arg1	G					300:300	immunoglobulin G	285:300	immunoglobulin G (IgG)	285:306	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	6	51	theme	ligand	1289:1294	arg1	Analysis					1268:1275	Analysis	1268:1275	Analysis of the IgG1 ligand bound to NK cell CD16a	1268:1317	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	2	52	theme	effector	471:478	arg1	function					480:487	increased effector function	461:487	increased effector function	461:487	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	3	53	theme	Fc	795:796	arg1	receptor					800:807	Fc γ receptor IIIa/CD16a	795:818	Fc γ receptor IIIa/CD16a	795:818	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	2	54	theme	bound	583:587	arg1	ligands					589:595	their bound ligands	577:595	their bound ligands	577:595	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	4	55	theme	CD16a	848:852	arg1	N162-glycans					854:865	CD16a N162-glycans	848:865	CD16a N162-glycans	848:865	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	2	56	theme	fucose	367:372	arg1	modification					374:385	a fucose modification	365:385	a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood	365:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	0	57	theme	Proinflammatory	42:56	arg1	IgG1					58:61	Proinflammatory IgG1	42:61	Proinflammatory IgG1 at the Cell Surface	42:81	Primary Human Natural Killer Cells Retain Proinflammatory IgG1 at the Cell Surface and Express CD16a Glycoforms with Donor-dependent Variability.					
31467031	3	58	theme	compositional	766:778	arg1	variability					780:790	the compositional variability	762:790	the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1	762:845	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	2	59	theme	surface	556:562	arg1	receptors					564:572	cell surface receptors	551:572	cell surface receptors	551:572	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	60	theme	increased	461:469	arg1	function					480:487	increased effector function	461:487	increased effector function	461:487	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	7	61	theme	unique	1468:1473	arg1	patterns					1488:1495	unique modification patterns	1468:1495	unique modification patterns	1468:1495	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	4	62	theme	variability	1091:1101	arg1	degree					1067:1072	a high degree	1060:1072	a high degree of compositional variability	1060:1101	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	2	63	from	abundance	620:628	arg1	blood					649:653	the circulating blood	633:653	the circulating blood	633:653	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	7	64	theme	Fc	1538:1539	arg1	modification					1548:1559	the Fc fucose modification	1534:1559	the Fc fucose modification	1534:1559	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	3	65	theme	NK	676:677	arg1	cells					679:683	primary NK cells	668:683	primary NK cells	668:683	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	6	66	theme	±	1425:1425	arg1	%					1430:1430	89.7 ± 3.9%	1420:1430	89.7 ± 3.9%	1420:1430	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	6	66	theme	±	1425:1425	arg1	donors					1412:1417	the same donors	1403:1417	the same donors (89.7 ± 3.9%)	1403:1431	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	4	67	theme	compositional	1077:1089	arg1	variability					1091:1101	compositional variability	1077:1101	compositional variability	1077:1101	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	6	68	theme	sharp	1330:1334	arg1	decrease					1336:1343	a sharp decrease	1328:1343	a sharp decrease in antibody fucosylation (43.2 ± 11.0%)	1328:1383	Analysis of the IgG1 ligand bound to NK cell CD16a revealed a sharp decrease in antibody fucosylation (43.2 ± 11.0%) versus serum from the same donors (89.7 ± 3.9%).					
31467031	2	69	theme	receptors	564:572	arg1	composition					536:546	the composition	532:546	the composition of cell surface receptors or their bound ligands in situ	532:603	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	70	theme	immunoglobulin	285:298	arg1	IgG					303:305	IgG	303:305	IgG	303:305	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	2	70	theme	immunoglobulin	285:298	arg1	G					300:300	immunoglobulin G	285:300	immunoglobulin G (IgG)	285:306	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	4	71	theme	largest	878:884	arg1	differences					886:896	the largest differences	874:896	the largest differences between donors	874:911	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	2	72	from	composition	536:546	arg1	situ					600:603	situ	600:603	situ	600:603	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	5	73	dep	N38	1239:1241	arg1	donors					1260:1265	all donors	1256:1265	all donors	1256:1265	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	7	74	theme	fucose	1541:1546	arg1	modification					1548:1559	the Fc fucose modification	1534:1559	the Fc fucose modification	1534:1559	Thus, NK cells express CD16a with unique modification patterns and preferentially bind IgG1 without the Fc fucose modification at the cell surface.					
31467031	1	75	theme	Post-translational	146:163	arg1	modification					165:176	Post-translational modification	146:176	Post-translational modification	146:176	Post-translational modification confers diverse functional properties to immune system proteins.					
31467031	5	76	with	N-glycans	1129:1137	arg1	processing					1157:1166	intermediate processing	1144:1166	intermediate processing	1144:1166	Hybrid-type N-glycans with intermediate processing dominated at N45 and highly modified, complex-type N-glycans decorated N38 and N74 from all donors.					
31467031	3	77	theme	γ	798:798	arg1	receptor					800:807	Fc γ receptor IIIa/CD16a	795:818	Fc γ receptor IIIa/CD16a	795:818	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	3	78	theme	apheresis	690:698	arg1	filters					700:706	apheresis filters	690:706	apheresis filters following plasma or platelet donation	690:744	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31467031	2	79	theme	-linked	436:442	arg1	glycan					444:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	the crystallizable fragment (Fc) asparagine(N)-linked glycan	390:449	The composition of serum proteins such as immunoglobulin G (IgG) strongly associates with disease including forms lacking a fucose modification of the crystallizable fragment (Fc) asparagine(N)-linked glycan that show increased effector function, however, virtually nothing is known about the composition of cell surface receptors or their bound ligands in situ because of low abundance in the circulating blood.					
31467031	4	80	theme	other	1037:1041	arg1	donors					1043:1048	the other donors	1033:1048	the other donors	1033:1048	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	4	81	theme	high	1062:1065	arg1	degree					1067:1072	a high degree	1060:1072	a high degree of compositional variability	1060:1101	CD16a N162-glycans showed the largest differences between donors; one donor displayed only oligomannose-type N-glycans at N162 that correlate with high affinity IgG1 Fc binding whereas the other donors displayed a high degree of compositional variability at this site.					
31467031	3	82	theme	receptor	800:807	arg1	variability					780:790	the compositional variability	762:790	the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1	762:845	We isolated primary NK cells from apheresis filters following plasma or platelet donation to characterize the compositional variability of Fc γ receptor IIIa/CD16a and its bound ligand, IgG1.					
31205022	2	0	theme	recombinant	356:366	arg1	Ps_Cel5A					368:375	recombinant Ps_Cel5A	356:375	recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily,	356:447	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	1	1	theme	carbohydrates	251:263	arg1	synthesis					238:246	the chemoenzymatic synthesis	219:246	the chemoenzymatic synthesis of carbohydrates	219:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	2	2	dep	Pseudomonas	382:392	arg1	stutzeri					394:401	stutzeri	394:401	stutzeri	394:401	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	4	3	theme	molecular	725:733	arg1	basis					735:739	a molecular basis	723:739	a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis	723:841	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	0	4	theme	molecular	100:108	arg1	basis					110:114	a molecular basis	98:114	a molecular basis for glycosynthesis in GH5_5 enzymes	98:150	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	0	5	theme	Crystal	0:6	arg1	determination					18:30	Crystal structure determination	0:30	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.	0:151	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	1	6	theme	new	170:172	arg1	hydrolases					184:193	new glycoside hydrolases	170:193	new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates	170:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	0	7	theme	structure	8:16	arg1	determination					18:30	Crystal structure determination	0:30	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.	0:151	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	1	8	theme	glycoside	174:182	arg1	hydrolases					184:193	new glycoside hydrolases	170:193	new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates	170:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	2	9	theme	subfamily	438:446	arg1	A1501					403:407	Pseudomonas stutzeri A1501	382:407	Pseudomonas stutzeri A1501	382:407	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	2	9	theme	subfamily	438:446	arg1	member					418:423	a novel member	410:423	a novel member of the GH5_5 subfamily	410:446	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	1	10	theme	hydrolases	184:193	arg1	discovery					157:165	The discovery	153:165	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates	153:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	2	11	theme	GH5_5	432:436	arg1	subfamily					438:446	the GH5_5 subfamily	428:446	the GH5_5 subfamily	428:446	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	4	12	theme	mechanisms	775:784	arg1	understanding					754:766	a better understanding	745:766	a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis	745:841	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	5	13	dep	residues	906:913	arg1	Lys216					927:932	Lys216	927:932	Lys216	927:932	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	13	dep	residues	906:913	arg1	Arg178					916:921	Arg178	916:921	Arg178	916:921	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	13	dep	residues	906:913	arg1	residues					906:913	two nonconserved positively charged residues	870:913	two nonconserved positively charged residues (Arg178 and Lys216)	870:933	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	14	theme	positive	851:858	arg1	subsites					860:867	the positive subsites	847:867	the positive subsites	847:867	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	1	15	theme	promising	282:290	arg1	approach					292:299	a promising approach	280:299	a promising approach for various biotechnological processes	280:338	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	4	16	theme	structural	652:661	arg1	determinants					663:674	several structural determinants	644:674	several structural determinants in and around the positive subsites	644:710	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	0	17	theme	GH5_5	138:142	arg1	enzymes					144:150	GH5_5 enzymes	138:150	GH5_5 enzymes	138:150	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	1	18	used	utilized	207:214	arg2	hydrolases					184:193	new glycoside hydrolases	170:193	new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates	170:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	2	19	theme	Pseudomonas	382:392	arg1	A1501					403:407	Pseudomonas stutzeri A1501	382:407	Pseudomonas stutzeri A1501	382:407	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	2	19	theme	Pseudomonas	382:392	arg1	member					418:423	a novel member	410:423	a novel member of the GH5_5 subfamily	410:446	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	2	20	theme	novel	412:416	arg1	A1501					403:407	Pseudomonas stutzeri A1501	382:407	Pseudomonas stutzeri A1501	382:407	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	2	20	theme	novel	412:416	arg1	member					418:423	a novel member	410:423	a novel member of the GH5_5 subfamily	410:446	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	0	21	theme	Pseudomonas	35:45	arg1	Cel5A					76:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	6	22	theme	transglycosylating	1018:1035	arg1	β-mannanases					1037:1048	transglycosylating β-mannanases	1018:1048	transglycosylating β-mannanases of the GH5_7 subfamily	1018:1071	This adaptation has also been reported for transglycosylating β-mannanases of the GH5_7 subfamily.					
31205022	0	23	from	glycosynthesis	120:133	arg1	enzymes					144:150	GH5_5 enzymes	138:150	GH5_5 enzymes	138:150	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	6	24	gly	transglycosylating	1018:1035	arg0	β-mannanases					1037:1048	transglycosylating β-mannanases	1018:1048	transglycosylating β-mannanases of the GH5_7 subfamily	1018:1071	This adaptation has also been reported for transglycosylating β-mannanases of the GH5_7 subfamily.					
31205022	5	25	theme	nonconserved	874:885	arg1	Lys216					927:932	Lys216	927:932	Lys216	927:932	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	25	theme	nonconserved	874:885	arg1	Arg178					916:921	Arg178	916:921	Arg178	916:921	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	25	theme	nonconserved	874:885	arg1	residues					906:913	two nonconserved positively charged residues	870:913	two nonconserved positively charged residues (Arg178 and Lys216)	870:933	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	0	26	theme	endoglucanase	62:74	arg1	Cel5A					76:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	1	27	theme	various	305:311	arg1	processes					330:338	various biotechnological processes	305:338	various biotechnological processes	305:338	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	0	28	theme	A1501	56:60	arg1	Cel5A					76:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Pseudomonas stutzeri A1501 endoglucanase Cel5A	35:80	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	1	29	theme	biotechnological	313:328	arg1	processes					330:338	various biotechnological processes	305:338	various biotechnological processes	305:338	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	0	30	dep	Pseudomonas	35:45	arg1	stutzeri					47:54	stutzeri	47:54	stutzeri	47:54	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	3	31	theme	Preliminary	491:501	arg1	experiments					503:513	Preliminary experiments	491:513	Preliminary experiments	491:513	Preliminary experiments confirmed the ability of Ps_Cel5A to catalyze transglycosylation with cellotriose as a substrate.					
31205022	4	32	theme	positive	694:701	arg1	subsites					703:710	the positive subsites	690:710	the positive subsites	690:710	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	2	33	from	A1501	403:407	arg1	Ps_Cel5A					368:375	recombinant Ps_Cel5A	356:375	recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily,	356:447	In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized.					
31205022	4	34	theme	better	747:752	arg1	understanding					754:766	a better understanding	745:766	a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis	745:841	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	6	35	theme	subfamily	1063:1071	arg1	β-mannanases					1037:1048	transglycosylating β-mannanases	1018:1048	transglycosylating β-mannanases of the GH5_7 subfamily	1018:1071	This adaptation has also been reported for transglycosylating β-mannanases of the GH5_7 subfamily.					
31205022	3	36	theme	Ps_Cel5A	540:547	arg1	ability					529:535	the ability	525:535	the ability of Ps_Cel5A to catalyze transglycosylation with cellotriose as a substrate	525:610	Preliminary experiments confirmed the ability of Ps_Cel5A to catalyze transglycosylation with cellotriose as a substrate.					
31205022	0	37	dep	determination	18:30	arg1	search					87:92	the search	83:92	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.	0:151	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	4	38	theme	crystal	617:623	arg1	structure					625:633	The crystal structure	613:633	The crystal structure	613:633	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	4	39	theme	several	644:650	arg1	determinants					663:674	several structural determinants	644:674	several structural determinants in and around the positive subsites	644:710	The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis.					
31205022	5	40	theme	charged	898:904	arg1	Lys216					927:932	Lys216	927:932	Lys216	927:932	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	40	theme	charged	898:904	arg1	Arg178					916:921	Arg178	916:921	Arg178	916:921	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	40	theme	charged	898:904	arg1	residues					906:913	two nonconserved positively charged residues	870:913	two nonconserved positively charged residues (Arg178 and Lys216)	870:933	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	1	41	theme	chemoenzymatic	223:236	arg1	synthesis					238:246	the chemoenzymatic synthesis	219:246	the chemoenzymatic synthesis of carbohydrates	219:263	The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes.					
31205022	0	42	theme	Cel5A	76:80	arg1	determination					18:30	Crystal structure determination	0:30	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.	0:151	Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.					
31205022	5	43	located	found	940:944	arg2	Arg178					916:921	Arg178	916:921	Arg178	916:921	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	43	located	found	940:944	arg1	subsites					860:867	the positive subsites	847:867	the positive subsites	847:867	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	43	located	found	940:944	arg2	residues					906:913	two nonconserved positively charged residues	870:913	two nonconserved positively charged residues (Arg178 and Lys216)	870:933	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
31205022	5	43	located	found	940:944	arg2	Lys216					927:932	Lys216	927:932	Lys216	927:932	In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose.					
30560888	8	0	theme	forms	1332:1336	arg1	abundances					1276:1285	even significantly lower abundances	1251:1285	even significantly lower abundances of bisected, afucosylated and galactosylated forms	1251:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	10	1	theme	specific	1573:1580	arg1	features					1582:1589	phenotype specific features	1563:1589	phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies	1563:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	3	2	theme	Jo1-	509:512	arg1	patients					514:521	Jo1+ versus Jo1- patients	497:521	Jo1+ versus Jo1- patients	497:521	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	10	3	theme	phenotype	1563:1571	arg1	features					1582:1589	phenotype specific features	1563:1589	phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies	1563:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	9	4	theme	Fc-glycan	1467:1475	arg1	features					1477:1484	Jo1+ characteristic Fc-glycan features	1447:1484	Jo1+ characteristic Fc-glycan features	1447:1484	ASS and ILD diagnosis, as well as lysozyme and thrombospondin correlated with Jo1+ characteristic Fc-glycan features.					
30560888	4	5	theme	high	729:732	arg1	abundance					734:742	a high abundance	727:742	a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44)	727:806	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	10	6	theme	pathogenic	1614:1623	arg1	role					1625:1628	the pathogenic role	1610:1628	the pathogenic role of Jo1 autoantibodies	1610:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	4	7	theme	healthy	820:826	arg1	n = 24					850:855	n = 24	850:855	n = 24	850:855	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	7	theme	healthy	820:826	arg1	controls					840:847	healthy age matched controls	820:847	healthy age matched controls (n = 24)	820:856	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	3	8	theme	Fc-glycan	599:607	arg1	features					609:616	particular Fc-glycan features	588:616	particular Fc-glycan features	588:616	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	3	9	theme	characteristic	551:564	arg1	differences					566:576	characteristic differences	551:576	characteristic differences	551:576	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	7	10	theme	Jo1-	1183:1186	arg1	patients					1188:1195	Jo1- patients	1183:1195	Jo1- patients	1183:1195	Bisected and afucosylated Fc-glycans were significantly lower in Jo1+ compared to Jo1- patients.					
30560888	0	11	theme	anti-Jo1	110:117	arg1	autoantibodies					119:132	anti-Jo1 autoantibodies	110:132	anti-Jo1 autoantibodies	110:132	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	3	12	theme	Jo1+	497:500	arg1	patients					514:521	Jo1+ versus Jo1- patients	497:521	Jo1+ versus Jo1- patients	497:521	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	5	13	theme	intra-individual	865:880	arg1	normalization					882:894	intra-individual normalization	865:894	intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	865:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	14	theme	FA2-IgG2	948:955	arg1	FA2					932:934	FA2	932:934	FA2	932:934	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	14	theme	FA2-IgG2	948:955	arg1	glycan					924:929	the main agalactosylated glycan	899:929	the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	899:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	2	15	theme	lung	420:423	arg1	ILD					434:436	ILD	434:436	ILD	434:436	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	15	theme	lung	420:423	arg1	disease					425:431	associated interstitial lung disease	396:431	associated interstitial lung disease (ILD)	396:437	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	7	16	theme	Bisected	1101:1108	arg1	Fc-glycans					1127:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans were significantly lower in Jo1+ compared to Jo1- patients.					
30560888	8	17	contain	contained	1241:1249	arg2	abundances					1276:1285	even significantly lower abundances	1251:1285	even significantly lower abundances of bisected, afucosylated and galactosylated forms	1251:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	8	17	contain	contained	1241:1249	arg1	Anti-Jo1-IgG					1198:1209	Anti-Jo1-IgG	1198:1209	Anti-Jo1-IgG enriched from eleven patients	1198:1239	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	8	18	theme	bisected	1290:1297	arg1	forms					1332:1336	bisected, afucosylated and galactosylated forms	1290:1336	bisected, afucosylated and galactosylated forms	1290:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	2	19	theme	interstitial	407:418	arg1	ILD					434:436	ILD	434:436	ILD	434:436	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	19	theme	interstitial	407:418	arg1	disease					425:431	associated interstitial lung disease	396:431	associated interstitial lung disease (ILD)	396:437	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	10	20	theme	autoantibodies	1637:1650	arg1	role					1625:1628	the pathogenic role	1610:1628	the pathogenic role of Jo1 autoantibodies	1610:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	3	21	from	patients	514:521	arg1	Fc-glycans					481:490	the total-IgG Fc-glycans	467:490	the total-IgG Fc-glycans from Jo1+ versus Jo1- patients	467:521	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	3	21	from	patients	514:521	arg1	anti-Jo1-IgG					527:538	anti-Jo1-IgG	527:538	anti-Jo1-IgG	527:538	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	4	22	theme	based	691:695	arg1	spectrometry					702:713	proteomics based mass spectrometry	680:713	proteomics based mass spectrometry	680:713	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	23	theme	ASS/IIM	782:788	arg1	patients					790:797	ASS/IIM patients	782:797	ASS/IIM patients (n = 44)	782:806	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	23	theme	ASS/IIM	782:788	arg1	n = 44					800:805	n = 44	800:805	n = 44	800:805	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	2	24	theme	associated	396:405	arg1	ILD					434:436	ILD	434:436	ILD	434:436	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	24	theme	associated	396:405	arg1	disease					425:431	associated interstitial lung disease	396:431	associated interstitial lung disease (ILD)	396:437	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	10	25	theme	Jo1	1633:1635	arg1	autoantibodies					1637:1650	Jo1 autoantibodies	1633:1650	Jo1 autoantibodies	1633:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	3	26	theme	particular	588:597	arg1	features					609:616	particular Fc-glycan features	588:616	particular Fc-glycan features	588:616	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	1	27	theme	autoimmune	189:198	arg1	diseases					200:207	autoimmune diseases	189:207	autoimmune diseases	189:207	IgG Fc-glycans affect IgG function and are altered in autoimmune diseases and autoantibodies.					
30560888	8	28	theme	afucosylated	1300:1311	arg1	forms					1332:1336	bisected, afucosylated and galactosylated forms	1290:1336	bisected, afucosylated and galactosylated forms	1290:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	10	29	theme	anti-Jo1+	1518:1526	arg1	profile					1546:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	2	30	theme	inflammatory	326:337	arg1	IIM					351:353	IIM	351:353	IIM	351:353	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	30	theme	inflammatory	326:337	arg1	myopathies					339:348	idiopathic inflammatory myopathies	315:348	idiopathic inflammatory myopathies (IIM)	315:354	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	6	31	theme	Jo1+	1049:1052	arg1	n = 19					1064:1069	n = 19	1064:1069	n = 19	1064:1069	For Jo1+ patients (n = 19) the AUCs went up to 88 ± 6%.					
30560888	6	31	theme	Jo1+	1049:1052	arg1	patients					1054:1061	Jo1+ patients	1049:1061	Jo1+ patients (n = 19)	1049:1070	For Jo1+ patients (n = 19) the AUCs went up to 88 ± 6%.					
30560888	0	32	theme	anti-Jo1	14:21	arg1	antibodies					23:32	anti-Jo1 antibodies	14:32	anti-Jo1 antibodies	14:32	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	2	33	theme	idiopathic	315:324	arg1	IIM					351:353	IIM	351:353	IIM	351:353	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	33	theme	idiopathic	315:324	arg1	myopathies					339:348	idiopathic inflammatory myopathies	315:348	idiopathic inflammatory myopathies (IIM)	315:354	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	8	34	gly	afucosylated	1300:1311	arg1	forms					1332:1336	bisected, afucosylated and galactosylated forms	1290:1336	bisected, afucosylated and galactosylated forms	1290:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	3	35	theme	total-IgG	471:479	arg1	Fc-glycans					481:490	the total-IgG Fc-glycans	467:490	the total-IgG Fc-glycans from Jo1+ versus Jo1- patients	467:521	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	7	36	theme	afucosylated	1114:1125	arg1	Fc-glycans					1127:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans were significantly lower in Jo1+ compared to Jo1- patients.					
30560888	5	37	theme	glycan	924:929	arg1	normalization					882:894	intra-individual normalization	865:894	intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	865:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	4	38	theme	proteomics	680:689	arg1	spectrometry					702:713	proteomics based mass spectrometry	680:713	proteomics based mass spectrometry	680:713	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	39	theme	mass	697:700	arg1	spectrometry					702:713	proteomics based mass spectrometry	680:713	proteomics based mass spectrometry	680:713	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	5	40	theme	main	903:906	arg1	FA2					932:934	FA2	932:934	FA2	932:934	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	40	theme	main	903:906	arg1	glycan					924:929	the main agalactosylated glycan	899:929	the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	899:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	41	theme	agalactosylated	908:922	arg1	FA2					932:934	FA2	932:934	FA2	932:934	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	41	theme	agalactosylated	908:922	arg1	glycan					924:929	the main agalactosylated glycan	899:929	the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	899:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	2	42	theme	synthetase	248:257	arg1	anti-Jo1					275:282	anti-Jo1	275:282	anti-Jo1	275:282	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	42	theme	synthetase	248:257	arg1	autoantibodies					259:272	Anti-histidyl tRNA synthetase autoantibodies	229:272	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1)	229:283	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	8	43	theme	lower	1270:1274	arg1	abundances					1276:1285	even significantly lower abundances	1251:1285	even significantly lower abundances of bisected, afucosylated and galactosylated forms	1251:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	0	44	theme	IgG	59:61	arg1	profile					73:79	a characteristic IgG Fc-glycan profile	42:79	a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies	42:132	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	9	45	theme	Jo1+	1447:1450	arg1	features					1477:1484	Jo1+ characteristic Fc-glycan features	1447:1484	Jo1+ characteristic Fc-glycan features	1447:1484	ASS and ILD diagnosis, as well as lysozyme and thrombospondin correlated with Jo1+ characteristic Fc-glycan features.					
30560888	8	46	theme	matched	1350:1356	arg1	total-IgG					1358:1366	matched total-IgG	1350:1366	matched total-IgG	1350:1366	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	2	47	theme	tRNA	243:246	arg1	synthetase					248:257	Anti-histidyl tRNA synthetase	229:257	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1)	229:283	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	10	48	theme	Fc-glycan	1536:1544	arg1	profile					1546:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	2	49	theme	anti-synthetase	360:374	arg1	ASS					386:388	ASS	386:388	ASS	386:388	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	49	theme	anti-synthetase	360:374	arg1	syndrome					376:383	anti-synthetase syndrome	360:383	anti-synthetase syndrome (ASS)	360:389	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	0	50	theme	characteristic	44:57	arg1	profile					73:79	a characteristic IgG Fc-glycan profile	42:79	a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies	42:132	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	9	51	theme	ILD	1377:1379	arg1	diagnosis					1381:1389	ILD diagnosis	1377:1389	ILD diagnosis	1377:1389	ASS and ILD diagnosis, as well as lysozyme and thrombospondin correlated with Jo1+ characteristic Fc-glycan features.					
30560888	5	52	theme	%	1042:1042	arg1	curve					1021:1025	the curve	1017:1025	the curve (AUC) of 79 ± 6%	1017:1042	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	52	theme	%	1042:1042	arg1	AUC					1028:1030	AUC	1028:1030	AUC	1028:1030	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	2	53	theme	Anti-histidyl	229:241	arg1	synthetase					248:257	Anti-histidyl tRNA synthetase	229:257	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1)	229:283	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	7	54	gly	afucosylated	1114:1125	arg1	Fc-glycans					1127:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans	1101:1136	Bisected and afucosylated Fc-glycans were significantly lower in Jo1+ compared to Jo1- patients.					
30560888	4	55	theme	agalactosylated	747:761	arg1	Fc-glycans					768:777	agalactosylated IgG1 Fc-glycans	747:777	agalactosylated IgG1 Fc-glycans	747:777	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	56	theme	age	828:830	arg1	n = 24					850:855	n = 24	850:855	n = 24	850:855	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	56	theme	age	828:830	arg1	controls					840:847	healthy age matched controls	820:847	healthy age matched controls (n = 24)	820:856	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	3	57	theme	specific	643:650	arg1	manifestations					661:674	specific clinical manifestations	643:674	specific clinical manifestations	643:674	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	0	58	with	Patients	0:7	arg1	antibodies					23:32	anti-Jo1 antibodies	14:32	anti-Jo1 antibodies	14:32	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	1	59	theme	IgG	135:137	arg1	Fc-glycans					139:148	IgG Fc-glycans	135:148	IgG Fc-glycans	135:148	IgG Fc-glycans affect IgG function and are altered in autoimmune diseases and autoantibodies.					
30560888	2	60	from	frequent	289:296	arg1	patients					301:308	patients	301:308	patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD)	301:437	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	10	61	theme	patient	1528:1534	arg1	profile					1546:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	5	62	theme	IgG1	940:943	arg1	FA2					932:934	FA2	932:934	FA2	932:934	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	5	62	theme	IgG1	940:943	arg1	glycan					924:929	the main agalactosylated glycan	899:929	the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2	899:955	Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2, ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%.					
30560888	3	63	theme	clinical	652:659	arg1	manifestations					661:674	specific clinical manifestations	643:674	specific clinical manifestations	643:674	Thus, we hypothesized that the total-IgG Fc-glycans from Jo1+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations.					
30560888	4	64	theme	IgG1	763:766	arg1	Fc-glycans					768:777	agalactosylated IgG1 Fc-glycans	747:777	agalactosylated IgG1 Fc-glycans	747:777	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	65	theme	Fc-glycans	768:777	arg1	abundance					734:742	a high abundance	727:742	a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44)	727:806	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	10	66	contain	contains	1554:1561	arg1	profile					1546:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	the anti-Jo1+ patient Fc-glycan profile	1514:1552	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	10	66	contain	contains	1554:1561	arg2	features					1582:1589	phenotype specific features	1563:1589	phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies	1563:1650	These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.					
30560888	8	67	theme	galactosylated	1317:1330	arg1	forms					1332:1336	bisected, afucosylated and galactosylated forms	1290:1336	bisected, afucosylated and galactosylated forms	1290:1336	Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG.					
30560888	2	68	from	patients	301:308	arg1	frequent					289:296	frequent	289:296	frequent	289:296	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	0	69	theme	Fc-glycan	63:71	arg1	profile					73:79	a characteristic IgG Fc-glycan profile	42:79	a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies	42:132	Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.					
30560888	9	70	theme	characteristic	1452:1465	arg1	features					1477:1484	Jo1+ characteristic Fc-glycan features	1447:1484	Jo1+ characteristic Fc-glycan features	1447:1484	ASS and ILD diagnosis, as well as lysozyme and thrombospondin correlated with Jo1+ characteristic Fc-glycan features.					
30560888	4	71	theme	matched	832:838	arg1	n = 24					850:855	n = 24	850:855	n = 24	850:855	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	71	theme	matched	832:838	arg1	controls					840:847	healthy age matched controls	820:847	healthy age matched controls (n = 24)	820:856	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	1	72	theme	IgG	157:159	arg1	function					161:168	IgG function	157:168	IgG function	157:168	IgG Fc-glycans affect IgG function and are altered in autoimmune diseases and autoantibodies.					
30560888	4	73	from	abundance	734:742	arg1	patients					790:797	ASS/IIM patients	782:797	ASS/IIM patients (n = 44)	782:806	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	4	73	from	abundance	734:742	arg1	n = 44					800:805	n = 44	800:805	n = 44	800:805	By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24).					
30560888	2	74	with	patients	301:308	arg1	myopathies					339:348	idiopathic inflammatory myopathies	315:348	idiopathic inflammatory myopathies (IIM)	315:354	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	74	with	patients	301:308	arg1	syndrome					376:383	anti-synthetase syndrome	360:383	anti-synthetase syndrome (ASS)	360:389	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	74	with	patients	301:308	arg1	ASS					386:388	ASS	386:388	ASS	386:388	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	74	with	patients	301:308	arg1	disease					425:431	associated interstitial lung disease	396:431	associated interstitial lung disease (ILD)	396:437	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	74	with	patients	301:308	arg1	IIM					351:353	IIM	351:353	IIM	351:353	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30560888	2	74	with	patients	301:308	arg1	ILD					434:436	ILD	434:436	ILD	434:436	Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD).					
30948514	0	0	theme	Ser/Thr-rich	68:79	arg1	domain					81:86	an N-terminal Ser/Thr-rich domain	54:86	an N-terminal Ser/Thr-rich domain conserved in vertebrates	54:111	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	3	1	gly	O-glycosylated	697:710	arg1	hOPSG					664:668	hOPSG	664:668	hOPSG from human retina	664:686	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	1	gly	O-glycosylated	697:710	arg1	hOPSR					654:658	hOPSR	654:658	hOPSR	654:658	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	5	2	from	retina	1072:1077	arg1	hOPSR					1045:1049	hOPSR	1045:1049	hOPSR	1045:1049	In addition, we show that hOPSR and hOPSG from human retina are recognized by jacalin, a lectin that binds to O-glycans, preferentially to Gal-GalNAc.					
30948514	5	2	from	retina	1072:1077	arg1	hOPSG					1055:1059	hOPSG	1055:1059	hOPSG	1055:1059	In addition, we show that hOPSR and hOPSG from human retina are recognized by jacalin, a lectin that binds to O-glycans, preferentially to Gal-GalNAc.					
30948514	7	3	theme	bovine	1332:1337	arg1	OPSR					1339:1342	bovine OPSR	1332:1342	bovine OPSR	1332:1342	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	3	4	theme	human	489:493	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	4	theme	human	489:493	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	4	theme	human	489:493	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	0	5	theme	N-terminal	57:66	arg1	domain					81:86	an N-terminal Ser/Thr-rich domain	54:86	an N-terminal Ser/Thr-rich domain conserved in vertebrates	54:111	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	6	6	from	presence	1193:1200	arg1	OPSG					1227:1230	OPSG	1227:1230	OPSG	1227:1230	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	6	from	presence	1193:1200	arg1	OPSR					1218:1221	OPSR	1218:1221	OPSR	1218:1221	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	8	7	theme	rod	1744:1746	arg1	photoreceptors					1757:1770	rod and cone photoreceptors	1744:1770	rod and cone photoreceptors	1744:1770	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	2	8	theme	outer	341:345	arg1	segments					347:354	rod and cone outer segments	328:354	rod and cone outer segments	328:354	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	4	9	theme	green	1000:1004	arg1	opsins					1011:1016	red and green cone opsins	992:1016	red and green cone opsins	992:1016	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	3	10	with	O-glycosylated	697:710	arg1	occupancy					722:730	full occupancy	717:730	full occupancy	717:730	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	2	11	theme	essential	280:288	arg1	proteins					299:306	the only essential membrane proteins	271:306	the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences	271:425	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	2	11	theme	essential	280:288	arg1	pigments					258:265	Visual pigments	251:265	Visual pigments	251:265	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	8	12	theme	red	1532:1534	arg1	opsins					1551:1556	red and green cone opsins	1532:1556	red and green cone opsins	1532:1556	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	7	13	theme	OPSR	1339:1342	arg1	analysis					1320:1327	the analysis	1316:1327	the analysis of bovine OPSR by MS	1316:1348	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	4	14	theme	hOPSR	811:815	arg1	21DSTQSSIF28					795:806	the N-terminal sequence 21DSTQSSIF28	771:806	the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina	771:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	1	15	from	differences	136:146	arg1	structures					155:164	the structures	151:164	the structures of outer segments	151:182	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	3	16	theme	cone	513:516	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	16	theme	cone	513:516	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	16	theme	cone	513:516	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	8	17	theme	opsins	1551:1556	arg1	feature					1521:1527	a fundamental feature	1507:1527	a fundamental feature	1507:1527	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	17	theme	opsins	1551:1556	arg1	relevant					1572:1579	relevant	1572:1579	relevant	1572:1579	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	17	theme	opsins	1551:1556	arg1	O-glycosylation					1488:1502	O-glycosylation	1488:1502	O-glycosylation	1488:1502	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	6	18	theme	vertebrate	1245:1254	arg1	species					1256:1262	several vertebrate species	1237:1262	several vertebrate species	1237:1262	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	18	theme	vertebrate	1245:1254	arg1	mammals					1275:1281	mammals	1275:1281	mammals	1275:1281	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	18	theme	vertebrate	1245:1254	arg1	amphibians					1295:1304	amphibians	1295:1304	amphibians	1295:1304	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	18	theme	vertebrate	1245:1254	arg1	birds					1284:1288	birds	1284:1288	birds	1284:1288	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	19	theme	O-glycans	1205:1213	arg1	presence					1193:1200	the presence	1189:1200	the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians	1189:1304	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	3	20	theme	red	499:501	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	20	theme	red	499:501	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	20	theme	red	499:501	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	21	gly	N-glycosylated	561:574	arg2	Asn34					579:583	Asn34	579:583	Asn34	579:583	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	21	gly	N-glycosylated	561:574	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	21	gly	N-glycosylated	561:574	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	21	gly	N-glycosylated	561:574	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	22	theme	Human	428:432	arg1	rhodopsin					434:442	Human rhodopsin	428:442	Human rhodopsin	428:442	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	6	23	theme	several	1237:1243	arg1	species					1256:1262	several vertebrate species	1237:1262	several vertebrate species	1237:1262	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	23	theme	several	1237:1243	arg1	mammals					1275:1281	mammals	1275:1281	mammals	1275:1281	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	23	theme	several	1237:1243	arg1	amphibians					1295:1304	amphibians	1295:1304	amphibians	1295:1304	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	23	theme	several	1237:1243	arg1	birds					1284:1288	birds	1284:1288	birds	1284:1288	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	8	24	theme	different	1718:1726	arg1	morphologies					1728:1739	the different morphologies	1714:1739	the different morphologies of rod and cone photoreceptors	1714:1770	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	25	theme	post-translational	1657:1674	arg1	modification					1676:1687	this post-translational modification	1652:1687	this post-translational modification	1652:1687	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	1	26	theme	outer	169:173	arg1	segments					175:182	outer segments	169:182	outer segments	169:182	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	0	27	theme	red	6:8	arg1	opsins					25:30	Human red and green cone opsins	0:30	Human red and green cone opsins	0:30	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	6	28	from	species	1256:1262	arg1	OPSG					1227:1230	OPSG	1227:1230	OPSG	1227:1230	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	28	from	species	1256:1262	arg1	OPSR					1218:1221	OPSR	1218:1221	OPSR	1218:1221	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	4	29	theme	sequence	786:793	arg1	21DSTQSSIF28					795:806	the N-terminal sequence 21DSTQSSIF28	771:806	the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina	771:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	8	30	theme	photoreceptors	1757:1770	arg1	morphologies					1728:1739	the different morphologies	1714:1739	the different morphologies of rod and cone photoreceptors	1714:1770	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	3	31	dep	human	489:493	arg1	h					496:496	h	496:496	h	496:496	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	32	from	Asn34	579:583	arg1	N-glycosylated					561:574	N-glycosylated	561:574	N-glycosylated	561:574	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	1	33	theme	segments	175:182	arg1	structures					155:164	the structures	151:164	the structures of outer segments	151:182	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	0	34	theme	Human	0:4	arg1	opsins					25:30	Human red and green cone opsins	0:30	Human red and green cone opsins	0:30	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	3	35	theme	human	675:679	arg1	retina					681:686	human retina	675:686	human retina	675:686	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	36	from	retina	681:686	arg1	hOPSG					664:668	hOPSG	664:668	hOPSG from human retina	664:686	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	36	from	retina	681:686	arg1	hOPSR					654:658	hOPSR	654:658	hOPSR	654:658	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	0	37	theme	green	14:18	arg1	opsins					25:30	Human red and green cone opsins	0:30	Human red and green cone opsins	0:30	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	4	38	theme	retina	850:855	arg1	extracts					832:839	extracts	832:839	extracts of human retina	832:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	2	39	theme	structural	404:413	arg1	differences					415:425	these structural differences	398:425	these structural differences	398:425	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	4	40	theme	post-translational	957:974	arg1	modification					976:987	this post-translational modification	952:987	this post-translational modification of red and green cone opsins	952:1016	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	4	41	theme	human	844:848	arg1	retina					850:855	human retina	844:855	human retina	844:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	2	42	theme	membrane	290:297	arg1	proteins					299:306	the only essential membrane proteins	271:306	the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences	271:425	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	2	42	theme	membrane	290:297	arg1	pigments					258:265	Visual pigments	251:265	Visual pigments	251:265	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	8	43	theme	cone	1546:1549	arg1	opsins					1551:1556	red and green cone opsins	1532:1556	red and green cone opsins	1532:1556	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	44	theme	green	1540:1544	arg1	opsins					1551:1556	red and green cone opsins	1532:1556	red and green cone opsins	1532:1556	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	45	theme	cone	1752:1755	arg1	photoreceptors					1757:1770	rod and cone photoreceptors	1744:1770	rod and cone photoreceptors	1744:1770	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	0	46	theme	cone	20:23	arg1	opsins					25:30	Human red and green cone opsins	0:30	Human red and green cone opsins	0:30	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	4	47	theme	O-glycosidase	912:924	arg1	treatment					926:934	O-glycosidase treatment	912:934	O-glycosidase treatment	912:934	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	2	48	dep	contribute	384:393	arg1	likely					367:372	likely	367:372	likely	367:372	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	1	49	theme	photoreceptor	205:217	arg1	cells					219:223	photoreceptor cells	205:223	photoreceptor cells	205:223	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	5	50	theme	human	1066:1070	arg1	retina					1072:1077	human retina	1066:1077	human retina	1066:1077	In addition, we show that hOPSR and hOPSG from human retina are recognized by jacalin, a lectin that binds to O-glycans, preferentially to Gal-GalNAc.					
30948514	3	51	gly	N-glycosylated	447:460	arg2	Asn2					465:468	Asn2	465:468	Asn2	465:468	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	51	gly	N-glycosylated	447:460	arg1	rhodopsin					434:442	Human rhodopsin	428:442	Human rhodopsin	428:442	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	51	gly	N-glycosylated	447:460	arg1	Asn15					474:478	Asn15	474:478	Asn15	474:478	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	51	gly	N-glycosylated	447:460	arg2	rhodopsin					434:442	Human rhodopsin	428:442	Human rhodopsin	428:442	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	51	gly	N-glycosylated	447:460	arg2	Asn15					474:478	Asn15	474:478	Asn15	474:478	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	51	gly	N-glycosylated	447:460	arg1	Asn2					465:468	Asn2	465:468	Asn2	465:468	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	4	52	theme	N-terminal	775:784	arg1	21DSTQSSIF28					795:806	the N-terminal sequence 21DSTQSSIF28	771:806	the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina	771:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	2	53	theme	cone	336:339	arg1	segments					347:354	rod and cone outer segments	328:354	rod and cone outer segments	328:354	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	7	54	from	O-glycan	1364:1371	arg1	Ser					1417:1419	Ser	1417:1419	Ser	1417:1419	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	54	from	O-glycan	1364:1371	arg1	Thr					1424:1426	Thr	1424:1426	Thr	1424:1426	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	54	from	O-glycan	1364:1371	arg1	Ser22					1376:1380	Ser22	1376:1380	Ser22	1376:1380	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	54	from	O-glycan	1364:1371	arg1	residue					1385:1391	a residue	1383:1391	a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG	1383:1458	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	54	from	O-glycan	1364:1371	arg1	semi-conserved					1401:1414	semi-conserved	1401:1414	semi-conserved	1401:1414	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	4	55	theme	hOPSG	821:825	arg1	21DSTQSSIF28					795:806	the N-terminal sequence 21DSTQSSIF28	771:806	the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina	771:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	4	56	theme	7G8	752:754	arg1	mAB					756:758	7G8 mAB	752:758	7G8 mAB	752:758	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	8	57	from	differences	1637:1647	arg1	modification					1676:1687	this post-translational modification	1652:1687	this post-translational modification	1652:1687	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	4	58	theme	opsins	1011:1016	arg1	modification					976:987	this post-translational modification	952:987	this post-translational modification of red and green cone opsins	952:1016	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	4	59	from	extracts	832:839	arg1	21DSTQSSIF28					795:806	the N-terminal sequence 21DSTQSSIF28	771:806	the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina	771:855	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	7	60	dep	semi-conserved	1401:1414	arg1	Ser22					1376:1380	Ser22	1376:1380	Ser22	1376:1380	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	60	dep	semi-conserved	1401:1414	arg1	Thr					1424:1426	Thr	1424:1426	Thr	1424:1426	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	60	dep	semi-conserved	1401:1414	arg1	Ser					1417:1419	Ser	1417:1419	Ser	1417:1419	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	60	dep	semi-conserved	1401:1414	arg1	residue					1385:1391	a residue	1383:1391	a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG	1383:1458	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	7	60	dep	semi-conserved	1401:1414	arg1	semi-conserved					1401:1414	semi-conserved	1401:1414	semi-conserved	1401:1414	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	2	61	theme	rod	328:330	arg1	segments					347:354	rod and cone outer segments	328:354	rod and cone outer segments	328:354	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	4	62	theme	cone	1006:1009	arg1	opsins					1011:1016	red and green cone opsins	992:1016	red and green cone opsins	992:1016	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	7	63	theme	vertebrate	1435:1444	arg1	OPSR					1446:1449	vertebrate OPSR	1435:1449	vertebrate OPSR	1435:1449	Finally, the analysis of bovine OPSR by MS identified an O-glycan on Ser22, a residue that is semi-conserved (Ser or Thr) among vertebrate OPSR and OPSG.					
30948514	3	64	theme	monoclonal	603:612	arg1	antibody					614:621	a monoclonal antibody	601:621	a monoclonal antibody (7G8 mAB)	601:631	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	64	theme	monoclonal	603:612	arg1	mAB					628:630	7G8 mAB	624:630	7G8 mAB	624:630	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	2	65	theme	Visual	251:256	arg1	proteins					299:306	the only essential membrane proteins	271:306	the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences	271:425	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	2	65	theme	Visual	251:256	arg1	pigments					258:265	Visual pigments	251:265	Visual pigments	251:265	Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences.					
30948514	8	66	theme	cell	1610:1613	arg1	development					1615:1625	cone cell development	1605:1625	cone cell development	1605:1625	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	1	67	theme	fundamental	124:134	arg1	differences					136:146	fundamental differences	124:146	fundamental differences in the structures of outer segments between rod and cone photoreceptor cells	124:223	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	6	68	attach	presence	1193:1200	arg1	OPSG					1227:1230	OPSG	1227:1230	OPSG	1227:1230	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	68	attach	presence	1193:1200	arg1	OPSR					1218:1221	OPSR	1218:1221	OPSR	1218:1221	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	6	68	attach	presence	1193:1200	arg2	O-glycans					1205:1213	O-glycans	1205:1213	O-glycans	1205:1213	Next, we confirmed the presence of O-glycans on OPSR and OPSG from several vertebrate species, including mammals, birds, and amphibians.					
30948514	0	69	from	domain	81:86	arg1	O-glycosylated					36:49	O-glycosylated	36:49	O-glycosylated	36:49	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	1	70	dep	rod	192:194	arg1	cells					219:223	photoreceptor cells	205:223	photoreceptor cells	205:223	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30948514	8	71	theme	cone	1605:1608	arg1	development					1615:1625	cone cell development	1605:1625	cone cell development	1605:1625	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	3	72	dep	opsins	518:523	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	72	dep	opsins	518:523	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	72	dep	opsins	518:523	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	8	73	theme	fundamental	1509:1519	arg1	feature					1521:1527	a fundamental feature	1507:1527	a fundamental feature	1507:1527	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	73	theme	fundamental	1509:1519	arg1	relevant					1572:1579	relevant	1572:1579	relevant	1572:1579	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	8	73	theme	fundamental	1509:1519	arg1	O-glycosylation					1488:1502	O-glycosylation	1488:1502	O-glycosylation	1488:1502	These results suggest that O-glycosylation is a fundamental feature of red and green cone opsins, which may be relevant to their function or to cone cell development, and that differences in this post-translational modification also could contribute to the different morphologies of rod and cone photoreceptors.					
30948514	3	74	theme	full	717:720	arg1	occupancy					722:730	full occupancy	717:730	full occupancy	717:730	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	75	theme	green	507:511	arg1	hOPSG					536:540	hOPSG	536:540	hOPSG	536:540	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	75	theme	green	507:511	arg1	hOPSR					526:530	hOPSR	526:530	hOPSR	526:530	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	3	75	theme	green	507:511	arg1	opsins					518:523	human (h) red and green cone opsins	489:523	human (h) red and green cone opsins (hOPSR and hOPSG, respectively)	489:555	Human rhodopsin is N-glycosylated on Asn2 and Asn15, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn34 Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy.					
30948514	0	76	gly	O-glycosylated	36:49	arg1	opsins					25:30	Human red and green cone opsins	0:30	Human red and green cone opsins	0:30	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	0	76	gly	O-glycosylated	36:49	arg2	domain					81:86	an N-terminal Ser/Thr-rich domain	54:86	an N-terminal Ser/Thr-rich domain conserved in vertebrates	54:111	Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.					
30948514	4	77	theme	red	992:994	arg1	opsins					1011:1016	red and green cone opsins	992:1016	red and green cone opsins	992:1016	We determined that 7G8 mAB recognizes the N-terminal sequence 21DSTQSSIF28 of hOPSR and hOPSG from extracts of human retina, but only after their O-glycans have been removed with O-glycosidase treatment, thus revealing this post-translational modification of red and green cone opsins.					
30948514	1	78	theme	vertebrate	232:241	arg1	retina					243:248	the vertebrate retina	228:248	the vertebrate retina	228:248	There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina.					
30508661	2	0	theme	unprotected	488:498	arg1	APP					526:528	APP	526:528	APP	526:528	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	0	theme	unprotected	488:498	arg1	phenyl					518:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl	470:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides	470:540	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	1	theme	spacer	610:615	arg1	aglycon					617:623	cleavable spacer aglycon	600:623	cleavable spacer aglycon	600:623	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	0	2	theme	phenyl	69:74	arg1	glycosides					105:114	4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides	50:114	4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides	50:114	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	2	3	theme	cleavable	600:608	arg1	aglycon					617:623	cleavable spacer aglycon	600:623	cleavable spacer aglycon	600:623	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	1	4	theme	glycosides	229:238	arg1	derivatives					182:192	per-O-acyl derivatives	171:192	per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	171:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	0	5	theme	4-	80:81	arg1	phenyl					98:103	phenyl	98:103	phenyl	98:103	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	2	6	theme	Janus	578:582	arg1	glycosides					584:593	next-generation Janus glycosides	562:593	next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation	562:672	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	0	7	dep	phenyl	69:74	arg1	3-chloropropoxy					53:67	3-chloropropoxy	53:67	3-chloropropoxy	53:67	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	8	theme	preparative	146:156	arg1	synthesis					158:166	the preparative synthesis	142:166	the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	142:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	2	9	theme	CPP	396:398	arg1	glycosides					400:409	The CPP glycosides	392:409	The CPP glycosides obtained	392:418	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	1	10	dep	mono-	262:266	arg1	d-glucosamine					353:365	d-glucosamine	353:365	d-glucosamine	353:365	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	d-mannose					311:319	d-mannose	311:319	d-mannose	311:319	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	l-rhamnose					322:331	l-rhamnose	322:331	l-rhamnose	322:331	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	lactose					368:374	lactose	368:374	lactose	368:374	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	d-glucose					287:295	d-glucose	287:295	d-glucose	287:295	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	d-galactose					298:308	d-galactose	298:308	d-galactose	298:308	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	10	dep	mono-	262:266	arg1	d-arabinofuranose					334:350	d-arabinofuranose	334:350	d-arabinofuranose	334:350	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	2	11	theme	next-generation	562:576	arg1	glycosides					584:593	next-generation Janus glycosides	562:593	next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation	562:672	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	0	12	theme	Janus	0:4	arg1	glycosides					6:15	Janus glycosides	0:15	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.	0:115	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	2	13	theme	ready	626:630	arg1	aglycon					617:623	cleavable spacer aglycon	600:623	cleavable spacer aglycon	600:623	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	0	14	theme	next	20:23	arg1	generation					25:34	next generation	20:34	next generation	20:34	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	15	theme	per-O-acyl	171:180	arg1	derivatives					182:192	per-O-acyl derivatives	171:192	per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	171:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	16	theme	series	245:250	arg1	glycosides					229:238	4-(3-chloropropoxy)phenyl (CPP) glycosides	197:238	4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	197:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	2	17	theme	3-azidopropoxy	503:516	arg1	APP					526:528	APP	526:528	APP	526:528	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	17	theme	3-azidopropoxy	503:516	arg1	phenyl					518:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl	470:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides	470:540	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	18	with	glycosides	584:593	arg1	aglycon					617:623	cleavable spacer aglycon	600:623	cleavable spacer aglycon	600:623	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	1	19	theme	derivatives	182:192	arg1	synthesis					158:166	the preparative synthesis	142:166	the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	142:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	0	20	theme	glycosides	105:114	arg1	Synthesis					37:45	Synthesis	37:45	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.	0:115	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	0	21	theme	generation	25:34	arg1	glycosides					6:15	Janus glycosides	0:15	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.	0:115	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	22	theme	common	255:260	arg1	mono-					262:266	common mono-	255:266	common mono-	255:266	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	0	23	theme	phenyl	98:103	arg1	glycosides					105:114	4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides	50:114	4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides	50:114	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	24	theme	mono-	262:266	arg1	series					245:250	a series	243:250	a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	243:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	2	25	theme	quantitative	447:458	arg1	yields					460:465	almost quantitative yields	440:465	almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation	440:672	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	26	theme	corresponding	474:486	arg1	APP					526:528	APP	526:528	APP	526:528	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	26	theme	corresponding	474:486	arg1	phenyl					518:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl	470:523	the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides	470:540	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	2	27	theme	further	651:657	arg1	transformation					659:672	further transformation	651:672	further transformation	651:672	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	1	28	theme	disaccharides	272:284	arg1	series					245:250	a series	243:250	a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	243:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	2	29	theme	phenyl	518:523	arg1	glycosides					531:540	the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides	470:540	the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides	470:540	The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation.					
30508661	0	30	dep	phenyl	98:103	arg1	3-azidopropoxy					83:96	3-azidopropoxy	83:96	3-azidopropoxy	83:96	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	31	theme	3-chloropropoxy	200:214	arg1	CPP					224:226	CPP	224:226	CPP	224:226	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	31	theme	3-chloropropoxy	200:214	arg1	phenyl					216:221	4-(3-chloropropoxy)phenyl	197:221	4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	197:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	0	32	dep	glycosides	6:15	arg1	Synthesis					37:45	Synthesis	37:45	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.	0:115	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	0	33	theme	4-	50:51	arg1	phenyl					69:74	phenyl	69:74	phenyl	69:74	Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.					
30508661	1	34	theme	Efficient	117:125	arg1	procedures					127:136	Efficient procedures	117:136	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	117:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
30508661	1	35	theme	phenyl	216:221	arg1	glycosides					229:238	4-(3-chloropropoxy)phenyl (CPP) glycosides	197:238	4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose)	197:375	Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described.					
31450448	6	0	theme	sialic	957:962	arg1	acids					964:968	sialic acids	957:968	sialic acids	957:968	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	9	1	theme	invasive	1344:1351	arg1	alternative					1353:1363	a minimally invasive alternative	1332:1363	a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows	1332:1430	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	1	2	theme	biomarkers	152:161	arg1	profiling					122:130	glyco-proteoform profiling	105:130	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	1	2	theme	biomarkers	152:161	arg1	analysis					85:92	Body fluid N-glycome analysis	64:92	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	6	3	theme	chemical	930:937	arg1	derivatization					939:952	chemical derivatization	930:952	chemical derivatization of sialic acids and MS-measurement of N-glycan profiles	930:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	5	4	theme	total	742:746	arg1	MS					784:785	MS	784:785	MS	784:785	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	5	4	theme	total	742:746	arg1	spectrometry					770:781	total plasma N-glycome mass spectrometry	742:781	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	7	5	theme	abundant	1134:1141	arg1	glycans					1143:1149	the ten most abundant glycans	1121:1149	the ten most abundant glycans	1121:1149	With this method, more than 80 different glycan structures are identified from a DBS, with RSDs below 10% for the ten most abundant glycans.					
31450448	10	6	theme	simplified	1438:1447	arg1	sampling					1455:1462	simplified blood sampling	1438:1462	simplified blood sampling	1438:1462	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	4	7	theme	N-glycosylation	592:606	arg1	profiling					608:616	facile N-glycosylation profiling	585:616	facile N-glycosylation profiling from DBS	585:625	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	4	8	from	DBS	623:625	arg1	profiling					608:616	facile N-glycosylation profiling	585:616	facile N-glycosylation profiling from DBS	585:625	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	9	9	theme	DBS	1319:1321	arg1	profiling					1306:1314	fast N-glycosylation profiling	1285:1314	fast N-glycosylation profiling of DBS	1285:1321	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	8	10	theme	venous	1194:1199	arg1	blood					1201:1205	venous blood	1194:1205	venous blood	1194:1205	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	9	11	dep	serum	1381:1385	arg1	workflows					1422:1430	protein N-glycosylation workflows	1398:1430	protein N-glycosylation workflows	1398:1430	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	2	12	theme	spot	327:330	arg1	collection					299:308	the collection	295:308	the collection of a dried blood spot (DBS)	295:336	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	2	12	theme	spot	327:330	arg1	alternative					368:378	an alternative	365:378	an alternative to sampling milliliters of peripheral blood	365:422	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	4	13	theme	sialic	654:659	arg1	linkage					666:672	sialic acid linkage	654:672	sialic acid linkage differentiation	654:688	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	9	14	theme	conventional	1368:1379	arg1	serum					1381:1385	conventional serum	1368:1385	conventional serum	1368:1385	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	3	15	theme	N-glycosylation	495:509	arg1	analysis					511:518	protein N-glycosylation analysis	487:518	protein N-glycosylation analysis aiming for high-throughput clinical applications	487:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	8	16	theme	blood	1201:1205	arg1	profiles					1161:1168	N-glycan profiles	1152:1168	N-glycan profiles of finger-tip blood and venous blood	1152:1205	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	3	17	theme	blood	457:461	arg1	strategy					474:481	a blood collection strategy	455:481	a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications	455:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	3	17	theme	blood	457:461	arg1	cards					446:450	DBS cards	442:450	DBS cards	442:450	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	1	18	theme	Body	64:67	arg1	analysis					85:92	Body fluid N-glycome analysis	64:92	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	8	19	theme	blood	1184:1188	arg1	profiles					1161:1168	N-glycan profiles	1152:1168	N-glycan profiles of finger-tip blood and venous blood	1152:1205	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	10	20	theme	glycomics	1514:1522	arg1	applications					1524:1535	clinical glycomics applications	1505:1535	clinical glycomics applications	1505:1535	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	10	21	theme	DBS	1469:1471	arg1	approach					1473:1480	this DBS approach	1464:1480	this DBS approach	1464:1480	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	1	22	theme	fluid	69:73	arg1	analysis					85:92	Body fluid N-glycome analysis	64:92	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	2	23	theme	sampling	383:390	arg1	milliliters					392:402	sampling milliliters	383:402	sampling milliliters of peripheral blood	383:422	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	0	24	theme	blood	6:10	arg1	analysis					27:34	blood spot N-glycome analysis	6:34	blood spot N-glycome analysis	6:34	Dried blood spot N-glycome analysis by MALDI mass spectrometry.					
31450448	6	25	theme	profiles	1001:1008	arg1	MS-measurement					974:987	MS-measurement	974:987	MS-measurement of N-glycan profiles	974:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	6	25	theme	profiles	1001:1008	arg1	acids					964:968	sialic acids	957:968	sialic acids	957:968	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	10	26	theme	clinical	1505:1512	arg1	applications					1524:1535	clinical glycomics applications	1505:1535	clinical glycomics applications	1505:1535	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	1	27	theme	N-glycome	75:83	arg1	analysis					85:92	Body fluid N-glycome analysis	64:92	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	1	28	theme	stratification	186:199	arg1	layer					201:205	a stratification layer	184:205	a stratification layer additional to quantitative, diagnostic protein levels	184:259	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	5	29	theme	DBS	833:835	arg1	specimens					837:845	DBS specimens	833:845	DBS specimens	833:845	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	9	30	theme	protein	1398:1404	arg1	workflows					1422:1430	protein N-glycosylation workflows	1398:1430	protein N-glycosylation workflows	1398:1430	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	2	31	theme	blood	321:325	arg1	DBS					333:335	DBS	333:335	DBS	333:335	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	2	31	theme	blood	321:325	arg1	spot					327:330	a dried blood spot	313:330	a dried blood spot (DBS)	313:336	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	10	32	theme	vast	1486:1489	arg1	potential					1491:1499	vast potential	1486:1499	vast potential for clinical glycomics applications	1486:1535	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	0	33	theme	N-glycome	17:25	arg1	analysis					27:34	blood spot N-glycome analysis	6:34	blood spot N-glycome analysis	6:34	Dried blood spot N-glycome analysis by MALDI mass spectrometry.					
31450448	5	34	theme	established	730:740	arg1	method					788:793	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	10	35	theme	blood	1449:1453	arg1	sampling					1455:1462	simplified blood sampling	1438:1462	simplified blood sampling	1438:1462	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	2	36	theme	dried	315:319	arg1	DBS					333:335	DBS	333:335	DBS	333:335	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	2	36	theme	dried	315:319	arg1	spot					327:330	a dried blood spot	313:330	a dried blood spot (DBS)	313:336	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	1	37	theme	additional	207:216	arg1	layer					201:205	a stratification layer	184:205	a stratification layer additional to quantitative, diagnostic protein levels	184:259	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	0	38	theme	spot	12:15	arg1	analysis					27:34	blood spot N-glycome analysis	6:34	blood spot N-glycome analysis	6:34	Dried blood spot N-glycome analysis by MALDI mass spectrometry.					
31450448	4	39	theme	facile	585:590	arg1	profiling					608:616	facile N-glycosylation profiling	585:616	facile N-glycosylation profiling from DBS	585:625	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	3	40	theme	high-throughput	531:545	arg1	applications					556:567	high-throughput clinical applications	531:567	high-throughput clinical applications	531:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	2	41	theme	blood	418:422	arg1	milliliters					392:402	sampling milliliters	383:402	sampling milliliters of peripheral blood	383:422	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	8	42	theme	short-term	1224:1233	arg1	stability					1235:1243	short-term stability	1224:1243	short-term stability of DBS	1224:1250	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	3	43	theme	protein	487:493	arg1	analysis					511:518	protein N-glycosylation analysis	487:518	protein N-glycosylation analysis aiming for high-throughput clinical applications	487:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	5	44	theme	plasma	748:753	arg1	MS					784:785	MS	784:785	MS	784:785	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	5	44	theme	plasma	748:753	arg1	spectrometry					770:781	total plasma N-glycome mass spectrometry	742:781	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	2	45	theme	peripheral	407:416	arg1	blood					418:422	peripheral blood	407:422	peripheral blood	407:422	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	1	46	theme	quantitative	221:232	arg1	levels					254:259	quantitative, diagnostic protein levels	221:259	quantitative, diagnostic protein levels	221:259	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	8	47	theme	DBS	1248:1250	arg1	stability					1235:1243	short-term stability	1224:1243	short-term stability of DBS	1224:1250	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	6	48	theme	N-glycan	992:999	arg1	profiles					1001:1008	N-glycan profiles	992:1008	N-glycan profiles	992:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	5	49	theme	N-glycome	755:763	arg1	MS					784:785	MS	784:785	MS	784:785	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	5	49	theme	N-glycome	755:763	arg1	spectrometry					770:781	total plasma N-glycome mass spectrometry	742:781	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	8	50	theme	N-glycan	1152:1159	arg1	profiles					1161:1168	N-glycan profiles	1152:1168	N-glycan profiles of finger-tip blood and venous blood	1152:1205	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	5	51	theme	specimens	837:845	arg1	analysis					821:828	the analysis	817:828	the analysis of DBS specimens	817:845	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	7	52	theme	glycan	1052:1057	arg1	structures					1059:1068	more than 80 different glycan structures	1029:1068	more than 80 different glycan structures	1029:1068	With this method, more than 80 different glycan structures are identified from a DBS, with RSDs below 10% for the ten most abundant glycans.					
31450448	4	53	theme	linkage	666:672	arg1	differentiation					674:688	sialic acid linkage differentiation	654:688	sialic acid linkage differentiation	654:688	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	0	54	theme	mass	45:48	arg1	spectrometry					50:61	MALDI mass spectrometry	39:61	MALDI mass spectrometry	39:61	Dried blood spot N-glycome analysis by MALDI mass spectrometry.					
31450448	5	55	theme	mass	765:768	arg1	MS					784:785	MS	784:785	MS	784:785	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	5	55	theme	mass	765:768	arg1	spectrometry					770:781	total plasma N-glycome mass spectrometry	742:781	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
31450448	10	56	contain	has	1482:1484	arg1	approach					1473:1480	this DBS approach	1464:1480	this DBS approach	1464:1480	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	10	56	contain	has	1482:1484	arg2	potential					1491:1499	vast potential	1486:1499	vast potential for clinical glycomics applications	1486:1535	With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.					
31450448	0	57	theme	MALDI	39:43	arg1	spectrometry					50:61	MALDI mass spectrometry	39:61	MALDI mass spectrometry	39:61	Dried blood spot N-glycome analysis by MALDI mass spectrometry.					
31450448	6	58	theme	MS-measurement	974:987	arg1	derivatization					939:952	chemical derivatization	930:952	chemical derivatization of sialic acids and MS-measurement of N-glycan profiles	930:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	9	59	theme	N-glycosylation	1290:1304	arg1	profiling					1306:1314	fast N-glycosylation profiling	1285:1314	fast N-glycosylation profiling of DBS	1285:1321	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	6	60	theme	protein	871:877	arg1	solubilization					879:892	DBS-punching and protein solubilization	854:892	solubilization	879:892	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	1	61	theme	glyco-proteoform	105:120	arg1	profiling					122:130	glyco-proteoform profiling	105:130	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers	64:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	9	62	theme	N-glycosylation	1406:1420	arg1	workflows					1422:1430	protein N-glycosylation workflows	1398:1430	protein N-glycosylation workflows	1398:1430	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	1	63	dep	quantitative	221:232	arg1	diagnostic					235:244	diagnostic	235:244	diagnostic	235:244	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	7	64	theme	different	1042:1050	arg1	structures					1059:1068	more than 80 different glycan structures	1029:1068	more than 80 different glycan structures	1029:1068	With this method, more than 80 different glycan structures are identified from a DBS, with RSDs below 10% for the ten most abundant glycans.					
31450448	3	65	theme	collection	463:472	arg1	strategy					474:481	a blood collection strategy	455:481	a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications	455:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	3	65	theme	collection	463:472	arg1	cards					446:450	DBS cards	442:450	DBS cards	442:450	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	8	66	theme	finger-tip	1173:1182	arg1	blood					1184:1188	finger-tip blood	1173:1188	finger-tip blood	1173:1188	N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated.					
31450448	3	67	theme	clinical	547:554	arg1	applications					556:567	high-throughput clinical applications	531:567	high-throughput clinical applications	531:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	6	68	theme	DBS-punching	854:865	arg1	solubilization					879:892	DBS-punching and protein solubilization	854:892	solubilization	879:892	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	2	69	theme	omics	275:279	arg1	applications					281:292	clinical omics applications	266:292	clinical omics applications	266:292	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	1	70	theme	protein	246:252	arg1	levels					254:259	quantitative, diagnostic protein levels	221:259	quantitative, diagnostic protein levels	221:259	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	9	71	theme	fast	1285:1288	arg1	profiling					1306:1314	fast N-glycosylation profiling	1285:1314	fast N-glycosylation profiling of DBS	1285:1321	This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows.					
31450448	2	72	theme	clinical	266:273	arg1	applications					281:292	clinical omics applications	266:292	clinical omics applications	266:292	For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood.					
31450448	4	73	theme	acid	661:664	arg1	linkage					666:672	sialic acid linkage	654:672	sialic acid linkage differentiation	654:688	A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation.					
31450448	1	74	theme	existing	135:142	arg1	biomarkers					152:161	existing protein biomarkers	135:161	existing protein biomarkers	135:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	3	75	theme	DBS	442:444	arg1	strategy					474:481	a blood collection strategy	455:481	a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications	455:567	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	3	75	theme	DBS	442:444	arg1	cards					446:450	DBS cards	442:450	DBS cards	442:450	Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications.					
31450448	6	76	theme	acids	964:968	arg1	derivatization					939:952	chemical derivatization	930:952	chemical derivatization of sialic acids and MS-measurement of N-glycan profiles	930:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	6	77	dep	released	908:915	arg1	followed					918:925	followed	918:925	followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles	918:1008	After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles.					
31450448	1	78	theme	protein	144:150	arg1	biomarkers					152:161	existing protein biomarkers	135:161	existing protein biomarkers	135:161	Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels.					
31450448	5	79	theme	spectrometry	770:781	arg1	method					788:793	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	a previously established total plasma N-glycome mass spectrometry (MS) method	717:793	This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens.					
29437979	17	0	theme	modulatory	2800:2809	arg1	role					2828:2831	a modulatory but nonessential role	2798:2831	a modulatory but nonessential role of N-glycans for gH function	2798:2860	Thus, our results demonstrate a modulatory but nonessential role of N-glycans for gH function.					
29437979	8	1	theme	conserved	1170:1178	arg1	N627					1180:1183	the conserved N627	1166:1183	the conserved N627	1166:1183	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	12	2	theme	gH	1800:1801	arg1	inactivation					1750:1761	even simultaneous inactivation	1732:1761	even simultaneous inactivation of all five N-glycosylation sites of gH	1732:1801	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	14	3	contain	have	2236:2239	arg2	impact					2250:2255	a strong impact	2241:2255	a strong impact	2241:2255	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	3	contain	have	2236:2239	arg1	N-glycans					2215:2223	N-glycans	2215:2223	N-glycans	2215:2223	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	11	4	theme	gH	1719:1720	arg1	localization					1686:1697	proper localization	1679:1697	proper localization	1679:1697	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	11	4	theme	gH	1719:1720	arg1	function					1703:1710	function	1703:1710	function	1703:1710	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	2	5	theme	functional	304:313	arg1	relevance					315:323	the functional relevance	300:323	the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery	300:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	12	6	theme	viral	1924:1928	arg1	envelope					1930:1937	the viral envelope	1920:1937	the viral envelope	1920:1937	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	16	7	theme	sites	2506:2510	arg1	mutation					2485:2492	mutation	2485:2492	mutation of specific sites	2485:2510	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	12	8	theme	gH/gL	2049:2053	arg1	complex					2055:2061	the heterodimeric gH/gL complex	2031:2061	the heterodimeric gH/gL complex	2031:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	1	9	theme	envelope	92:99	arg1	proteins					101:108	Many viral envelope proteins	81:108	Many viral envelope proteins	81:108	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	11	10	from	role	1654:1657	arg1	localization					1686:1697	proper localization	1679:1697	proper localization	1679:1697	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	11	10	from	role	1654:1657	arg1	function					1703:1710	function	1703:1710	function	1703:1710	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	9	11	theme	endoplasmic	1401:1411	arg1	ER					1424:1425	ER	1424:1425	ER	1424:1425	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	9	11	theme	endoplasmic	1401:1411	arg1	reticulum					1413:1421	endoplasmic reticulum	1401:1421	endoplasmic reticulum (ER) retention	1401:1436	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	8	12	theme	PrV-specific	1146:1157	arg1	N77					1159:1161	the PrV-specific N77	1142:1161	the PrV-specific N77	1142:1161	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	16	13	theme	gH	2521:2522	arg1	transport					2524:2532	gH transport	2521:2532	gH transport	2521:2532	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	8	14	theme	in	1219:1220	arg1	activity					1235:1242	significantly reduced in vitro fusion activity	1197:1242	significantly reduced in vitro fusion activity	1197:1242	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	15	15	link	N-linked	2381:2388	arg1	sites					2404:2408	all five predicted N-linked glycosylation sites	2362:2408	all five predicted N-linked glycosylation sites	2362:2408	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	15	16	theme	sites	2404:2408	arg1	relevance					2349:2357	the functional relevance	2334:2357	the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH	2334:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	1	17	theme	physicochemical	200:214	arg1	properties					216:225	physicochemical properties	200:225	physicochemical properties	200:225	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	16	18	theme	sites	2690:2694	arg1	inactivation					2649:2660	even simultaneous inactivation	2631:2660	even simultaneous inactivation of all five N-glycosylation sites of gH	2631:2700	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	8	19	theme	fusion	1228:1233	arg1	activity					1235:1242	significantly reduced in vitro fusion activity	1197:1242	significantly reduced in vitro fusion activity	1197:1242	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	10	20	theme	Varicellovirus	1527:1540	arg1	genus					1542:1546	the Varicellovirus genus	1523:1546	the Varicellovirus genus	1523:1546	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	3	21	theme	gL	570:571	arg1	complex					552:558	the heterodimeric complex	534:558	the heterodimeric complex of gH and gL	534:571	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	15	22	theme	N-linked	2381:2388	arg1	sites					2404:2408	all five predicted N-linked glycosylation sites	2362:2408	all five predicted N-linked glycosylation sites	2362:2408	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	16	23	theme	gH	2699:2700	arg1	inactivation					2649:2660	even simultaneous inactivation	2631:2660	even simultaneous inactivation of all five N-glycosylation sites of gH	2631:2700	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	4	24	theme	potential	688:696	arg1	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	11	25	theme	N-glycans	1666:1674	arg1	role					1654:1657	a role	1652:1657	a role of the N-glycans in proper localization and function of PrV gH	1652:1720	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	2	26	theme	fusion	481:486	arg1	machinery					488:496	the conserved core herpesvirus fusion machinery	450:496	the conserved core herpesvirus fusion machinery	450:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	8	27	theme	penetration	1253:1263	arg1	kinetics					1265:1272	delayed penetration kinetics	1245:1272	delayed penetration kinetics	1245:1272	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	0	28	theme	Glycoprotein	64:75	arg1	gH					77:78	Pseudorabies Virus Glycoprotein gH	45:78	Pseudorabies Virus Glycoprotein gH	45:78	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	15	29	theme	functional	2338:2347	arg1	relevance					2349:2357	the functional relevance	2334:2357	the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH	2334:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	9	30	theme	surface	1450:1456	arg1	expression					1458:1467	reduced surface expression	1442:1467	reduced surface expression	1442:1467	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	2	31	theme	core	464:467	arg1	machinery					488:496	the conserved core herpesvirus fusion machinery	450:496	the conserved core herpesvirus fusion machinery	450:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	32	from	relevance	315:323	arg1	component					437:445	an essential component	424:445	an essential component of the conserved core herpesvirus fusion machinery	424:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	32	from	relevance	315:323	arg1	gH					410:411	gH	410:411	gH	410:411	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	32	from	relevance	315:323	arg1	H					407:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	8	33	theme	smaller	1279:1285	arg1	plaques					1293:1299	smaller virus plaques	1279:1299	smaller virus plaques	1279:1299	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	11	34	theme	proper	1679:1684	arg1	localization					1686:1697	proper localization	1679:1697	proper localization	1679:1697	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	4	35	gly	glycosylation	707:719	arg2	five					683:686	five	683:686	five	683:686	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	4	35	gly	glycosylation	707:719	arg2	positions					730:738	positions 77, 162, 542, 604, and 627	730:765	positions 77, 162, 542, 604, and 627	730:765	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	4	35	gly	glycosylation	707:719	arg2	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	12	36	theme	infectious	1841:1850	arg1	virus					1852:1856	infectious virus	1841:1856	infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1841:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	2	37	theme	glycoprotein	394:405	arg1	component					437:445	an essential component	424:445	an essential component of the conserved core herpesvirus fusion machinery	424:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	37	theme	glycoprotein	394:405	arg1	gH					410:411	gH	410:411	gH	410:411	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	37	theme	glycoprotein	394:405	arg1	H					407:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	15	38	theme	pseudorabies	2434:2445	arg1	PrV					2454:2456	PrV	2454:2456	PrV	2454:2456	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	15	38	theme	pseudorabies	2434:2445	arg1	virus					2447:2451	alphaherpesvirus pseudorabies virus	2417:2451	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	1	39	theme	Many	81:84	arg1	proteins					101:108	Many viral envelope proteins	81:108	Many viral envelope proteins	81:108	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	6	40	theme	PrV	1004:1006	arg1	genome					1008:1013	the PrV genome	1000:1013	the PrV genome	1000:1013	Additionally, the mutated gH genes were inserted into the PrV genome for analysis of function during virus infection.					
29437979	4	41	theme	glycosylation	707:719	arg1	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	10	42	theme	enhanced	1561:1568	arg1	activity					1586:1593	enhanced in vitro fusion activity	1561:1593	enhanced in vitro fusion activity	1561:1593	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	0	43	theme	Functional	0:9	arg1	Role					11:14	Functional Role	0:14	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.	0:79	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	5	44	theme	mutated	873:879	arg1	proteins					881:888	The mutated proteins	869:888	The mutated proteins	869:888	The mutated proteins were tested for correct expression and fusion activity.					
29437979	1	45	mod	modified	114:121	arg3	glycosylation					148:160	asparagine (N)-linked glycosylation	126:160	asparagine (N)-linked glycosylation	126:160	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	1	45	mod	modified	114:121	arg1	proteins					101:108	Many viral envelope proteins	81:108	Many viral envelope proteins	81:108	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	1	46	link	-linked	140:146	arg1	glycosylation					148:160	asparagine (N)-linked glycosylation	126:160	asparagine (N)-linked glycosylation	126:160	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	10	47	theme	viral	1599:1603	arg1	spread					1618:1623	viral cell-to-cell spread	1599:1623	viral cell-to-cell spread	1599:1623	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	16	48	dep	in	2535:2536	arg1	vitro					2538:2542	vitro	2538:2542	vitro	2538:2542	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	12	49	theme	N-glycosylation	1775:1789	arg1	sites					1791:1795	all five N-glycosylation sites	1766:1795	all five N-glycosylation sites	1766:1795	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	12	50	theme	fusion	1993:1998	arg1	machinery					2000:2008	the conserved fusion machinery	1979:2008	the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1979:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	0	51	theme	Glycosylation	28:40	arg1	Role					11:14	Functional Role	0:14	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.	0:79	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	13	52	theme	gH/gL	2127:2131	arg1	complex					2133:2139	the gH/gL complex	2123:2139	the gH/gL complex	2123:2139	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	6	53	theme	gH	972:973	arg1	genes					975:979	the mutated gH genes	960:979	the mutated gH genes	960:979	Additionally, the mutated gH genes were inserted into the PrV genome for analysis of function during virus infection.					
29437979	3	54	theme	viral	611:615	arg1	envelope					617:624	the viral envelope	607:624	the viral envelope	607:624	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	0	55	theme	Pseudorabies	45:56	arg1	gH					77:78	Pseudorabies Virus Glycoprotein gH	45:78	Pseudorabies Virus Glycoprotein gH	45:78	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	12	56	theme	simultaneous	1737:1748	arg1	inactivation					1750:1761	even simultaneous inactivation	1732:1761	even simultaneous inactivation of all five N-glycosylation sites of gH	1732:1801	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	2	57	theme	N-linked	328:335	arg1	glycans					337:343	N-linked glycans	328:343	N-linked glycans	328:343	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	5	58	theme	fusion	929:934	arg1	activity					936:943	fusion activity	929:943	fusion activity	929:943	The mutated proteins were tested for correct expression and fusion activity.					
29437979	12	59	theme	conserved	1983:1991	arg1	machinery					2000:2008	the conserved fusion machinery	1979:2008	the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1979:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	3	60	theme	host	635:638	arg1	membrane					645:652	the host cell membrane	631:652	the host cell membrane for viral entry	631:668	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	2	61	theme	virus	382:386	arg1	component					437:445	an essential component	424:445	an essential component of the conserved core herpesvirus fusion machinery	424:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	61	theme	virus	382:386	arg1	gH					410:411	gH	410:411	gH	410:411	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	61	theme	virus	382:386	arg1	H					407:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	1	62	theme	intracellular	228:240	arg1	transport					242:250	intracellular transport	228:250	intracellular transport	228:250	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	4	63	dep	mutations	807:815	arg1	asparagine					818:827	asparagine	818:827	asparagine to glutamine	818:840	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	12	64	theme	Herpesvirus	1879:1889	arg1	infection					1891:1899	particles.IMPORTANCE Herpesvirus infection	1858:1899	particles.IMPORTANCE Herpesvirus infection	1858:1899	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	14	65	gly	glycoproteins	2172:2184	arg1	gH					2195:2196	gH	2195:2196	gH	2195:2196	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	65	gly	glycoproteins	2172:2184	arg1	glycoproteins					2172:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	13	66	dep	bona	2068:2071	arg1	fide					2073:2076	fide	2073:2076	fide	2073:2076	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	14	67	theme	Viral	2157:2161	arg1	gH					2195:2196	gH	2195:2196	gH	2195:2196	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	67	theme	Viral	2157:2161	arg1	glycoproteins					2172:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	68	contain	contain	2207:2213	arg2	N-glycans					2215:2223	N-glycans	2215:2223	N-glycans	2215:2223	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	68	contain	contain	2207:2213	arg1	gH					2195:2196	gH	2195:2196	gH	2195:2196	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	68	contain	contain	2207:2213	arg1	glycoproteins					2172:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	2	69	theme	alphaherpesvirus	352:367	arg1	PrV					389:391	PrV	389:391	PrV	389:391	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	69	theme	alphaherpesvirus	352:367	arg1	virus					382:386	alphaherpesvirus pseudorabies virus	352:386	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	13	70	theme	protein	2085:2091	arg1	gB					2093:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	4	71	theme	conservative	794:805	arg1	mutations					807:815	conservative mutations	794:815	conservative mutations (asparagine to glutamine) singly or in combination	794:866	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	4	72	link	N-linked	698:705	arg1	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	3	73	theme	viral	658:662	arg1	entry					664:668	viral entry	658:668	viral entry	658:668	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	9	74	theme	gH	1363:1364	arg1	transport					1350:1358	transport	1350:1358	transport of gH in transfected cells	1350:1385	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	12	75	theme	envelope	1930:1937	arg1	fusion					1910:1915	fusion	1910:1915	fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1910:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	10	76	theme	N604	1495:1498	arg1	mutation					1483:1490	mutation	1483:1490	mutation	1483:1490	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	15	77	from	relevance	2349:2357	arg1	gH					2459:2460	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	3	78	theme	receptor	516:523	arg1	binding					525:531	gD-mediated receptor binding	504:531	gD-mediated receptor binding	504:531	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	9	79	theme	transfected	1369:1379	arg1	cells					1381:1385	transfected cells	1369:1385	transfected cells	1369:1385	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	12	80	theme	cellular	1944:1951	arg1	membranes					1953:1961	cellular membranes	1944:1961	cellular membranes	1944:1961	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	4	81	contain	contains	674:681	arg1	gH					671:672	gH	671:672	gH	671:672	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	4	81	contain	contains	674:681	arg2	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	16	82	theme	infectious	2740:2749	arg1	particles					2757:2765	infectious virus particles	2740:2765	infectious virus particles	2740:2765	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	8	83	theme	N627	1180:1183	arg1	Inactivation					1126:1137	Inactivation	1126:1137	Inactivation of the PrV-specific N77 or the conserved N627	1126:1183	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	16	84	gly	N-glycosylation	2674:2688	arg2	sites					2690:2694	all five N-glycosylation sites	2665:2694	all five N-glycosylation sites	2665:2694	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	16	84	gly	N-glycosylation	2674:2688	arg2	five					2669:2672	five	2669:2672	five	2669:2672	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	16	85	theme	virus	2751:2755	arg1	particles					2757:2765	infectious virus particles	2740:2765	infectious virus particles	2740:2765	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	3	86	theme	heterodimeric	538:550	arg1	complex					552:558	the heterodimeric complex	534:558	the heterodimeric complex of gH and gL	534:571	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	3	87	theme	gH	563:564	arg1	complex					552:558	the heterodimeric complex	534:558	the heterodimeric complex of gH and gL	534:571	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	16	88	theme	particles	2757:2765	arg1	formation					2727:2735	formation	2727:2735	formation of infectious virus particles	2727:2765	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	12	89	theme	heterodimeric	2035:2047	arg1	complex					2055:2061	the heterodimeric gH/gL complex	2031:2061	the heterodimeric gH/gL complex	2031:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	12	90	dep	virus	1852:1856	arg1	requires					1901:1908	requires	1901:1908	requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1901:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	11	91	theme	PrV	1715:1717	arg1	gH					1719:1720	PrV gH	1715:1720	PrV gH	1715:1720	These results demonstrate a role of the N-glycans in proper localization and function of PrV gH.					
29437979	13	92	theme	bona	2068:2071	arg1	gB					2093:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	9	93	theme	reticulum	1413:1421	arg1	retention					1428:1436	endoplasmic reticulum (ER) retention	1401:1436	endoplasmic reticulum (ER) retention	1401:1436	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	12	94	theme	particles.IMPORTANCE	1858:1877	arg1	infection					1891:1899	particles.IMPORTANCE Herpesvirus infection	1858:1899	particles.IMPORTANCE Herpesvirus infection	1858:1899	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	16	95	theme	in	2535:2536	arg1	activity					2551:2558	in vitro fusion activity	2535:2558	in vitro fusion activity	2535:2558	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	8	96	theme	reduced	1211:1217	arg1	activity					1235:1242	significantly reduced in vitro fusion activity	1197:1242	significantly reduced in vitro fusion activity	1197:1242	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	13	97	theme	fusion	2078:2083	arg1	gB					2093:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB	2064:2094	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	8	98	theme	N77	1159:1161	arg1	Inactivation					1126:1137	Inactivation	1126:1137	Inactivation of the PrV-specific N77 or the conserved N627	1126:1183	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	16	99	theme	fusion	2544:2549	arg1	activity					2551:2558	in vitro fusion activity	2535:2558	in vitro fusion activity	2535:2558	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	16	100	theme	N-glycosylation	2674:2688	arg1	sites					2690:2694	all five N-glycosylation sites	2665:2694	all five N-glycosylation sites	2665:2694	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	15	101	theme	glycosylation	2390:2402	arg1	sites					2404:2408	all five predicted N-linked glycosylation sites	2362:2408	all five predicted N-linked glycosylation sites	2362:2408	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	6	102	theme	mutated	964:970	arg1	genes					975:979	the mutated gH genes	960:979	the mutated gH genes	960:979	Additionally, the mutated gH genes were inserted into the PrV genome for analysis of function during virus infection.					
29437979	14	103	theme	strong	2243:2248	arg1	impact					2250:2255	a strong impact	2241:2255	a strong impact	2241:2255	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	16	104	theme	cell-to-cell	2565:2576	arg1	spread					2578:2583	cell-to-cell spread	2565:2583	cell-to-cell spread	2565:2583	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	15	105	theme	predicted	2371:2379	arg1	sites					2404:2408	all five predicted N-linked glycosylation sites	2362:2408	all five predicted N-linked glycosylation sites	2362:2408	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	9	106	theme	reduced	1442:1448	arg1	expression					1458:1467	reduced surface expression	1442:1467	reduced surface expression	1442:1467	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	2	107	theme	herpesvirus	469:479	arg1	machinery					488:496	the conserved core herpesvirus fusion machinery	450:496	the conserved core herpesvirus fusion machinery	450:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	8	108	theme	delayed	1245:1251	arg1	kinetics					1265:1272	delayed penetration kinetics	1245:1272	delayed penetration kinetics	1245:1272	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	3	109	with	fusion	597:602	arg1	membrane					645:652	the host cell membrane	631:652	the host cell membrane for viral entry	631:668	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	1	110	theme	-linked	140:146	arg1	glycosylation					148:160	asparagine (N)-linked glycosylation	126:160	asparagine (N)-linked glycosylation	126:160	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	2	111	theme	conserved	454:462	arg1	machinery					488:496	the conserved core herpesvirus fusion machinery	450:496	the conserved core herpesvirus fusion machinery	450:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	0	112	from	Role	11:14	arg1	gH					77:78	Pseudorabies Virus Glycoprotein gH	45:78	Pseudorabies Virus Glycoprotein gH	45:78	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	12	113	with	fusion	1910:1915	arg1	membranes					1953:1961	cellular membranes	1944:1961	cellular membranes	1944:1961	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	12	114	theme	virus	1852:1856	arg1	formation					1828:1836	formation	1828:1836	formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex	1828:2061	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	8	115	dep	in	1219:1220	arg1	vitro					1222:1226	vitro	1222:1226	vitro	1222:1226	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	16	116	theme	specific	2497:2504	arg1	sites					2506:2510	specific sites	2497:2510	specific sites	2497:2510	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	12	117	gly	N-glycosylation	1775:1789	arg2	sites					1791:1795	all five N-glycosylation sites	1766:1795	all five N-glycosylation sites	1766:1795	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	12	117	gly	N-glycosylation	1775:1789	arg2	five					1770:1773	five	1770:1773	five	1770:1773	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	10	118	theme	cell-to-cell	1605:1616	arg1	spread					1618:1623	viral cell-to-cell spread	1599:1623	viral cell-to-cell spread	1599:1623	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	4	119	from	positions	730:738	arg1	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	5	120	theme	correct	906:912	arg1	expression					914:923	correct expression	906:923	correct expression	906:923	The mutated proteins were tested for correct expression and fusion activity.					
29437979	16	121	theme	penetration	2609:2619	arg1	kinetics					2621:2628	delayed penetration kinetics	2601:2628	delayed penetration kinetics	2601:2628	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	2	122	theme	machinery	488:496	arg1	component					437:445	an essential component	424:445	an essential component of the conserved core herpesvirus fusion machinery	424:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	122	theme	machinery	488:496	arg1	H					407:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	123	gly	glycoprotein	394:405	arg1	glycoprotein					394:405	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	8	124	theme	virus	1287:1291	arg1	plaques					1293:1299	smaller virus plaques	1279:1299	smaller virus plaques	1279:1299	Inactivation of the PrV-specific N77 or the conserved N627 resulted in significantly reduced in vitro fusion activity, delayed penetration kinetics, and smaller virus plaques.					
29437979	10	125	theme	in	1570:1571	arg1	activity					1586:1593	enhanced in vitro fusion activity	1561:1593	enhanced in vitro fusion activity	1561:1593	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	15	126	theme	virus	2447:2451	arg1	gH					2459:2460	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	1	127	theme	viral	86:90	arg1	proteins					101:108	Many viral envelope proteins	81:108	Many viral envelope proteins	81:108	Many viral envelope proteins are modified by asparagine (N)-linked glycosylation, which can influence their structure, physicochemical properties, intracellular transport, and function.					
29437979	4	128	theme	N-linked	698:705	arg1	sites					721:725	five potential N-linked glycosylation sites	683:725	five potential N-linked glycosylation sites	683:725	gH contains five potential N-linked glycosylation sites at positions 77, 162, 542, 604, and 627, which were inactivated by conservative mutations (asparagine to glutamine) singly or in combination.					
29437979	17	129	theme	N-glycans	2836:2844	arg1	role					2828:2831	a modulatory but nonessential role	2798:2831	a modulatory but nonessential role of N-glycans for gH function	2798:2860	Thus, our results demonstrate a modulatory but nonessential role of N-glycans for gH function.					
29437979	0	130	theme	N-Linked	19:26	arg1	Glycosylation					28:40	N-Linked Glycosylation	19:40	N-Linked Glycosylation	19:40	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	12	131	theme	sites	1791:1795	arg1	inactivation					1750:1761	even simultaneous inactivation	1732:1761	even simultaneous inactivation of all five N-glycosylation sites of gH	1732:1801	However, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellular membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex.					
29437979	15	132	theme	alphaherpesvirus	2417:2432	arg1	PrV					2454:2456	PrV	2454:2456	PrV	2454:2456	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	15	132	theme	alphaherpesvirus	2417:2432	arg1	virus					2447:2451	alphaherpesvirus pseudorabies virus	2417:2451	the alphaherpesvirus pseudorabies virus (PrV) gH	2413:2460	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	16	133	theme	simultaneous	2636:2647	arg1	inactivation					2649:2660	even simultaneous inactivation	2631:2660	even simultaneous inactivation of all five N-glycosylation sites of gH	2631:2700	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	3	134	theme	envelope	617:624	arg1	fusion					597:602	fusion	597:602	fusion of the viral envelope with the host cell membrane for viral entry	597:668	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	17	135	theme	gH	2850:2851	arg1	function					2853:2860	gH function	2850:2860	gH function	2850:2860	Thus, our results demonstrate a modulatory but nonessential role of N-glycans for gH function.					
29437979	9	136	from	transport	1350:1358	arg1	cells					1381:1385	transfected cells	1369:1385	transfected cells	1369:1385	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	17	137	theme	nonessential	2815:2826	arg1	role					2828:2831	a modulatory but nonessential role	2798:2831	a modulatory but nonessential role of N-glycans for gH function	2798:2860	Thus, our results demonstrate a modulatory but nonessential role of N-glycans for gH function.					
29437979	10	138	theme	fusion	1579:1584	arg1	activity					1586:1593	enhanced in vitro fusion activity	1561:1593	enhanced in vitro fusion activity	1561:1593	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	0	139	theme	Virus	58:62	arg1	gH					77:78	Pseudorabies Virus Glycoprotein gH	45:78	Pseudorabies Virus Glycoprotein gH	45:78	Functional Role of N-Linked Glycosylation in Pseudorabies Virus Glycoprotein gH.					
29437979	13	140	theme	complex	2133:2139	arg1	presence					2111:2118	the presence	2107:2118	the presence of the gH/gL complex for activation	2107:2154	The bona fide fusion protein gB depends on the presence of the gH/gL complex for activation.					
29437979	2	141	theme	essential	427:435	arg1	component					437:445	an essential component	424:445	an essential component of the conserved core herpesvirus fusion machinery	424:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	141	theme	essential	427:435	arg1	H					407:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H	348:407	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	15	142	gly	glycosylation	2390:2402	arg2	sites					2404:2408	all five predicted N-linked glycosylation sites	2362:2408	all five predicted N-linked glycosylation sites	2362:2408	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	15	142	gly	glycosylation	2390:2402	arg2	five					2366:2369	five	2366:2369	five	2366:2369	Here, we systematically analyzed the functional relevance of all five predicted N-linked glycosylation sites in the alphaherpesvirus pseudorabies virus (PrV) gH.					
29437979	3	143	theme	cell	640:643	arg1	membrane					645:652	the host cell membrane	631:652	the host cell membrane for viral entry	631:668	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
29437979	2	144	theme	glycans	337:343	arg1	relevance					315:323	the functional relevance	300:323	the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery	300:496	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	6	145	theme	virus	1047:1051	arg1	infection					1053:1061	virus infection	1047:1061	virus infection	1047:1061	Additionally, the mutated gH genes were inserted into the PrV genome for analysis of function during virus infection.					
29437979	7	146	gly	glycosylated	1112:1123	arg1	sites					1102:1106	all five sites	1093:1106	all five sites	1093:1106	Our results demonstrate that all five sites are glycosylated.					
29437979	2	147	theme	pseudorabies	369:380	arg1	PrV					389:391	PrV	389:391	PrV	389:391	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	2	147	theme	pseudorabies	369:380	arg1	virus					382:386	alphaherpesvirus pseudorabies virus	352:386	the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH)	348:412	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	9	148	theme	N627	1328:1331	arg1	substitution					1312:1323	substitution	1312:1323	substitution of N627	1312:1331	Moreover, substitution of N627 greatly affected transport of gH in transfected cells, resulting in endoplasmic reticulum (ER) retention and reduced surface expression.					
29437979	6	149	theme	function	1031:1038	arg1	analysis					1019:1026	analysis	1019:1026	analysis of function during virus infection	1019:1061	Additionally, the mutated gH genes were inserted into the PrV genome for analysis of function during virus infection.					
29437979	16	150	theme	delayed	2601:2607	arg1	kinetics					2621:2628	delayed penetration kinetics	2601:2628	delayed penetration kinetics	2601:2628	Despite the fact that mutation of specific sites affected gH transport, in vitro fusion activity, and cell-to-cell spread and resulted in delayed penetration kinetics, even simultaneous inactivation of all five N-glycosylation sites of gH did not severely inhibit formation of infectious virus particles.					
29437979	14	151	theme	envelope	2163:2170	arg1	gH					2195:2196	gH	2195:2196	gH	2195:2196	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	14	151	theme	envelope	2163:2170	arg1	glycoproteins					2172:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins	2157:2184	Viral envelope glycoproteins, such as gH, usually contain N-glycans, which can have a strong impact on their folding, transport, and functions.					
29437979	2	152	link	N-linked	328:335	arg1	glycans					337:343	N-linked glycans	328:343	N-linked glycans	328:343	Here, we systematically analyzed the functional relevance of N-linked glycans in the alphaherpesvirus pseudorabies virus (PrV) glycoprotein H (gH), which is an essential component of the conserved core herpesvirus fusion machinery.					
29437979	10	153	dep	in	1570:1571	arg1	vitro					1573:1577	vitro	1573:1577	vitro	1573:1577	In contrast, mutation of N604, which is conserved in the Varicellovirus genus, resulted in enhanced in vitro fusion activity and viral cell-to-cell spread.					
29437979	3	154	theme	gD-mediated	504:514	arg1	binding					525:531	gD-mediated receptor binding	504:531	gD-mediated receptor binding	504:531	Upon gD-mediated receptor binding, the heterodimeric complex of gH and gL activates gB to mediate fusion of the viral envelope with the host cell membrane for viral entry.					
31375533	5	0	theme	Many	638:641	arg1	glycosyltransferases					643:662	Many glycosyltransferases	638:662	Many glycosyltransferases for N-glycan terminals	638:685	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	4	1	theme	epitopes	468:475	arg1	Expression					448:457	Expression	448:457	Expression of these epitopes in N-glycan	448:487	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	0	2	theme	N-glycan	69:76	arg1	Modification					53:64	Terminal Modification	44:64	Terminal Modification of N-glycan	44:76	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	6	3	theme	GnT-III-deficient	910:926	arg1	mice					928:931	GnT-III-deficient mice	910:931	GnT-III-deficient mice	910:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	5	4	dep	revealed	692:699	arg1	whereas					778:784	whereas	778:784	whereas	778:784	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	6	5	theme	epitopes	898:905	arg1	expression					862:871	the elevated expression	849:871	the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice	849:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	3	6	theme	terminal	358:365	arg1	epitopes					367:374	terminal epitopes	358:374	terminal epitopes in N-glycans	358:387	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	7	from	epitopes	367:374	arg1	N-glycans					379:387	N-glycans	379:387	N-glycans	379:387	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	5	8	theme	N-glycan	668:675	arg1	terminals					677:685	N-glycan terminals	668:685	N-glycan terminals	668:685	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	6	9	theme	enzymes	996:1002	arg1	specificity					964:974	the substrate specificity	950:974	the substrate specificity of the biosynthetic enzymes	950:1002	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	3	10	theme	various	341:347	arg1	types					349:353	various types	341:353	various types	341:353	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	10	theme	various	341:347	arg1	acid					415:418	sialic acid	408:418	sialic acid	408:418	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	10	theme	various	341:347	arg1	killer-1					438:445	human natural killer-1	424:445	human natural killer-1	424:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	10	theme	various	341:347	arg1	fucose					400:405	fucose	400:405	fucose	400:405	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	6	11	theme	N-glycan	889:896	arg1	epitopes					898:905	the terminal N-glycan epitopes	876:905	the terminal N-glycan epitopes in GnT-III-deficient mice	876:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	3	12	theme	human	424:428	arg1	killer-1					438:445	human natural killer-1	424:445	human natural killer-1	424:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	13	theme	types	349:353	arg1	biosynthesis					325:336	the biosynthesis	321:336	the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1	321:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	14	from	N-glycans	379:387	arg1	types					349:353	various types	341:353	various types	341:353	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	14	from	N-glycans	379:387	arg1	acid					415:418	sialic acid	408:418	sialic acid	408:418	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	14	from	N-glycans	379:387	arg1	killer-1					438:445	human natural killer-1	424:445	human natural killer-1	424:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	14	from	N-glycans	379:387	arg1	fucose					400:405	fucose	400:405	fucose	400:405	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	4	15	from	Expression	448:457	arg1	N-glycan					480:487	N-glycan	480:487	N-glycan	480:487	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	5	16	theme	mRNAs	812:816	arg1	up-regulation					789:801	no up-regulation	786:801	no up-regulation of their mRNAs	786:816	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	3	17	theme	natural	430:436	arg1	killer-1					438:445	human natural killer-1	424:445	human natural killer-1	424:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	6	18	from	mice	928:931	arg1	expression					862:871	the elevated expression	849:871	the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice	849:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	6	19	theme	biosynthetic	983:994	arg1	enzymes					996:1002	the biosynthetic enzymes	979:1002	the biosynthetic enzymes	979:1002	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	0	20	theme	Bisecting	0:8	arg1	Suppressor					30:39	a General Suppressor	20:39	a General Suppressor of Terminal Modification of N-glycan	20:76	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	0	20	theme	Bisecting	0:8	arg1	GlcNAc					10:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	4	21	theme	bisecting	545:553	arg1	GlcNAc					555:560	bisecting GlcNAc	545:560	bisecting GlcNAc	545:560	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	3	22	theme	epitopes	367:374	arg1	types					349:353	various types	341:353	various types	341:353	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	22	theme	epitopes	367:374	arg1	acid					415:418	sialic acid	408:418	sialic acid	408:418	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	22	theme	epitopes	367:374	arg1	killer-1					438:445	human natural killer-1	424:445	human natural killer-1	424:445	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	22	theme	epitopes	367:374	arg1	fucose					400:405	fucose	400:405	fucose	400:405	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	3	23	theme	branching	273:281	arg1	residue					289:295	a branching sugar residue	271:295	a branching sugar residue in N-glycan	271:307	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	6	24	from	expression	862:871	arg1	mice					928:931	GnT-III-deficient mice	910:931	GnT-III-deficient mice	910:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	3	25	theme	sugar	283:287	arg1	residue					289:295	a branching sugar residue	271:295	a branching sugar residue in N-glycan	271:307	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	2	26	theme	complex	185:191	arg1	biosynthesis					200:211	complex glycan biosynthesis	185:211	complex glycan biosynthesis	185:211	However, regulation mechanisms of complex glycan biosynthesis are largely unclear.					
31375533	8	27	theme	N-glycosylation	1199:1213	arg1	mechanism					1178:1186	a new regulation mechanism	1161:1186	a new regulation mechanism of protein N-glycosylation	1161:1213	These findings unveil a new regulation mechanism of protein N-glycosylation.					
31375533	4	28	theme	GlcNAc	555:560	arg1	GnT-III					563:569	GnT-III	563:569	GnT-III	563:569	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	4	28	theme	GlcNAc	555:560	arg1	enzyme					535:540	the biosynthetic enzyme	518:540	the biosynthetic enzyme of bisecting GlcNAc	518:560	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	5	29	theme	nonbisected	713:723	arg1	substrate					739:747	a substrate	737:747	a substrate to its bisected counterpart	737:775	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	5	29	theme	nonbisected	713:723	arg1	N-glycan					725:732	a nonbisected N-glycan	711:732	a nonbisected N-glycan	711:732	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	3	30	from	types	349:353	arg1	N-glycans					379:387	N-glycans	379:387	N-glycans	379:387	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	4	31	from	overexpression	613:626	arg1	cells					631:635	cells	631:635	cells	631:635	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	0	32	theme	General	22:28	arg1	Suppressor					30:39	a General Suppressor	20:39	a General Suppressor of Terminal Modification of N-glycan	20:76	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	0	32	theme	General	22:28	arg1	GlcNAc					10:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	6	33	theme	substrate	954:962	arg1	specificity					964:974	the substrate specificity	950:974	the substrate specificity of the biosynthetic enzymes	950:1002	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	3	34	theme	sialic	408:413	arg1	acid					415:418	sialic acid	408:418	sialic acid	408:418	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	6	35	theme	elevated	853:860	arg1	expression					862:871	the elevated expression	849:871	the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice	849:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	0	36	theme	Terminal	44:51	arg1	Modification					53:64	Terminal Modification	44:64	Terminal Modification of N-glycan	44:76	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	8	37	theme	new	1163:1165	arg1	mechanism					1178:1186	a new regulation mechanism	1161:1186	a new regulation mechanism of protein N-glycosylation	1161:1213	These findings unveil a new regulation mechanism of protein N-glycosylation.					
31375533	2	38	theme	regulation	160:169	arg1	mechanisms					171:180	regulation mechanisms	160:180	regulation mechanisms of complex glycan biosynthesis	160:211	However, regulation mechanisms of complex glycan biosynthesis are largely unclear.					
31375533	6	39	theme	terminal	880:887	arg1	epitopes					898:905	the terminal N-glycan epitopes	876:905	the terminal N-glycan epitopes in GnT-III-deficient mice	876:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	1	40	theme	complex	112:118	arg1	glycans					120:126	complex glycans	112:126	complex glycans for protein functions	112:148	Glycoproteins are decorated with complex glycans for protein functions.					
31375533	4	41	theme	biosynthetic	522:533	arg1	GnT-III					563:569	GnT-III	563:569	GnT-III	563:569	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	4	41	theme	biosynthetic	522:533	arg1	enzyme					535:540	the biosynthetic enzyme	518:540	the biosynthetic enzyme of bisecting GlcNAc	518:560	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	7	42	theme	nonbisected	1059:1069	arg1	glycans					1071:1077	nonbisected glycans	1059:1077	nonbisected glycans	1059:1077	Molecular dynamics simulations further confirmed that nonbisected glycans were preferentially accepted by those glycosyltransferases.					
31375533	6	43	from	epitopes	898:905	arg1	mice					928:931	GnT-III-deficient mice	910:931	GnT-III-deficient mice	910:931	This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes.					
31375533	5	44	theme	bisected	756:763	arg1	counterpart					765:775	its bisected counterpart	752:775	its bisected counterpart	752:775	Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found.					
31375533	3	45	from	residue	289:295	arg1	N-glycan					300:307	N-glycan	300:307	N-glycan	300:307	Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1.					
31375533	8	46	theme	regulation	1167:1176	arg1	mechanism					1178:1186	a new regulation mechanism	1161:1186	a new regulation mechanism of protein N-glycosylation	1161:1213	These findings unveil a new regulation mechanism of protein N-glycosylation.					
31375533	2	47	theme	biosynthesis	200:211	arg1	mechanisms					171:180	regulation mechanisms	160:180	regulation mechanisms of complex glycan biosynthesis	160:211	However, regulation mechanisms of complex glycan biosynthesis are largely unclear.					
31375533	7	48	theme	Molecular	1005:1013	arg1	simulations					1024:1034	Molecular dynamics simulations	1005:1034	Molecular dynamics simulations	1005:1034	Molecular dynamics simulations further confirmed that nonbisected glycans were preferentially accepted by those glycosyltransferases.					
31375533	1	49	theme	protein	132:138	arg1	functions					140:148	protein functions	132:148	protein functions	132:148	Glycoproteins are decorated with complex glycans for protein functions.					
31375533	8	50	theme	protein	1191:1197	arg1	N-glycosylation					1199:1213	protein N-glycosylation	1191:1213	protein N-glycosylation	1191:1213	These findings unveil a new regulation mechanism of protein N-glycosylation.					
31375533	2	51	theme	glycan	193:198	arg1	biosynthesis					200:211	complex glycan biosynthesis	185:211	complex glycan biosynthesis	185:211	However, regulation mechanisms of complex glycan biosynthesis are largely unclear.					
31375533	7	52	theme	dynamics	1015:1022	arg1	simulations					1024:1034	Molecular dynamics simulations	1005:1034	Molecular dynamics simulations	1005:1034	Molecular dynamics simulations further confirmed that nonbisected glycans were preferentially accepted by those glycosyltransferases.					
31375533	4	53	theme	GnT-III	605:611	arg1	overexpression					613:626	GnT-III overexpression	605:626	GnT-III overexpression in cells	605:635	Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells.					
31375533	0	54	theme	Modification	53:64	arg1	Suppressor					30:39	a General Suppressor	20:39	a General Suppressor of Terminal Modification of N-glycan	20:76	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
31375533	0	54	theme	Modification	53:64	arg1	GlcNAc					10:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc	0:15	Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.					
30651366	14	0	theme	cell	2565:2568	arg1	E2					2570:2571	both mammalian and insect cell E2	2539:2571	both mammalian and insect cell E2	2539:2571	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	5	1	dep	cells	814:818	arg1	i					792:792	i	792:792	i	792:792	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	7	2	theme	conformation-dependent	1206:1227	arg1	antibodies					1229:1238	conformation-dependent antibodies	1206:1238	conformation-dependent antibodies	1206:1238	Both proteins bound CD81 and were recognized by conformation-dependent antibodies.					
30651366	17	3	theme	complete	3006:3013	arg1	approach					2966:2973	A more productive approach	2948:2973	A more productive approach for vaccine development	2948:2997	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	17	3	theme	complete	3006:3013	arg1	deletion					3015:3022	complete deletion	3006:3022	complete deletion of specific glycans	3006:3042	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	6	4	link	Sf9-derived	1066:1076	arg1	sE2					1078:1080	Sf9-derived sE2	1066:1080	Sf9-derived sE2	1066:1080	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	14	5	theme	Mass	2452:2455	arg1	spectrometry					2457:2468	Mass spectrometry	2452:2468	Mass spectrometry	2452:2468	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	10	6	theme	overall	1769:1775	arg1	immunogenicity					1777:1790	overall immunogenicity	1769:1790	overall immunogenicity	1769:1790	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	9	7	theme	Sf9-derived	1427:1437	arg1	sE2					1439:1441	Sf9-derived sE2	1427:1441	Sf9-derived sE2	1427:1441	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	16	8	theme	neutralizing	2767:2778	arg1	responses					2789:2797	stronger neutralizing antibody responses	2758:2797	stronger neutralizing antibody responses	2758:2797	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	16	9	theme	neutralization	2895:2908	arg1	titers					2910:2915	comparable neutralization titers	2884:2915	comparable neutralization titers against heterologous isolates	2884:2945	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	10	10	dep	modification	1924:1935	arg1	development					2002:2012	The development	1998:2012	just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV)	1919:2053	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	5	11	theme	head-to-head	696:707	arg1	comparisons					747:757	head-to-head molecular, antigenic, and immunogenic comparisons	696:757	head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	696:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	3	12	theme	viral	562:566	arg1	escape					568:573	viral escape	562:573	viral escape	562:573	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	11	13	theme	HCV	2192:2194	arg1	E2					2214:2215	E2	2214:2215	E2	2214:2215	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	11	13	theme	HCV	2192:2194	arg1	protein					2205:2211	the HCV envelope protein	2188:2211	the HCV envelope protein	2188:2211	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	9	14	link	Sf9-derived	1427:1437	arg1	sE2					1439:1441	Sf9-derived sE2	1427:1441	Sf9-derived sE2	1427:1441	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	15	15	theme	polyclonal	2695:2704	arg1	responses					2715:2723	similar polyclonal antibody responses	2687:2723	similar polyclonal antibody responses	2687:2723	Mouse immunogenicity studies revealed similar polyclonal antibody responses.					
30651366	5	16	theme	high-mannose-type	854:870	arg1	glycans					872:878	high-mannose-type glycans	854:878	high-mannose-type glycans	854:878	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	14	17	gly	glycosylation	2502:2514	arg2	sites					2516:2520	the predicted glycosylation sites	2488:2520	the predicted glycosylation sites	2488:2520	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	8	18	theme	Mouse	1241:1245	arg1	studies					1262:1268	Mouse immunogenicity studies	1241:1268	Mouse immunogenicity studies	1241:1268	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	10	19	theme	C	2041:2041	arg1	HCV					2050:2052	HCV	2050:2052	HCV	2050:2052	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	19	theme	C	2041:2041	arg1	virus					2043:2047	hepatitis C virus	2031:2047	hepatitis C virus (HCV)	2031:2053	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	20	theme	antibody	1838:1845	arg1	deletion					1897:1904	complete deletion	1888:1904	complete deletion	1888:1904	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	20	theme	antibody	1838:1845	arg1	response					1847:1854	the antibody response	1834:1854	the antibody response to neutralizing epitopes	1834:1879	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	1	21	theme	C	199:199	arg1	HCV					208:210	HCV	208:210	HCV	208:210	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	1	21	theme	C	199:199	arg1	virus					201:205	hepatitis C virus	189:205	hepatitis C virus (HCV)	189:211	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	11	22	theme	vaccine	2112:2118	arg1	development					2120:2130	vaccine development	2112:2130	vaccine development	2112:2130	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	4	23	theme	E2	666:667	arg1	antigenicity					631:642	antigenicity	631:642	antigenicity	631:642	We hypothesized that glycosylation might influence the antigenicity and immunogenicity of E2.					
30651366	4	23	theme	E2	666:667	arg1	immunogenicity					648:661	immunogenicity	648:661	immunogenicity	648:661	We hypothesized that glycosylation might influence the antigenicity and immunogenicity of E2.					
30651366	5	24	theme	immunogenic	735:745	arg1	comparisons					747:757	head-to-head molecular, antigenic, and immunogenic comparisons	696:757	head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	696:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	2	25	theme	antibodies	295:304	arg1	importance					281:290	the importance	277:290	the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance	277:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	15	26	theme	immunogenicity	2655:2668	arg1	studies					2670:2676	Mouse immunogenicity studies	2649:2676	Mouse immunogenicity studies	2649:2676	Mouse immunogenicity studies revealed similar polyclonal antibody responses.					
30651366	9	27	theme	HEK293-derived	1490:1503	arg1	sE2					1505:1507	HEK293-derived sE2	1490:1507	HEK293-derived sE2 against the homologous HCV H77c isolate	1490:1547	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	17	28	theme	specific	3027:3034	arg1	glycans					3036:3042	specific glycans	3027:3042	specific glycans	3027:3042	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	10	29	theme	HCV	1672:1674	arg1	glycosylation					1679:1691	HCV E2 glycosylation	1672:1691	HCV E2 glycosylation	1672:1691	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	8	30	theme	polyclonal	1292:1301	arg1	responses					1312:1320	similar polyclonal antibody responses	1284:1320	similar polyclonal antibody responses	1284:1320	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	9	31	theme	homologous	1521:1530	arg1	isolate					1541:1547	the homologous HCV H77c isolate	1517:1547	the homologous HCV H77c isolate	1517:1547	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	10	32	theme	health	2072:2077	arg1	challenge					2079:2087	a global health challenge	2063:2087	a global health challenge	2063:2087	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	4	33	dep	antigenicity	631:642	arg1	the					627:629	the	627:629	the	627:629	We hypothesized that glycosylation might influence the antigenicity and immunogenicity of E2.					
30651366	2	34	theme	Cumulative	248:257	arg1	evidence					259:266	Cumulative evidence	248:266	Cumulative evidence	248:266	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	9	35	theme	H77c	1536:1539	arg1	isolate					1541:1547	the homologous HCV H77c isolate	1517:1547	the homologous HCV H77c isolate	1517:1547	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	6	36	theme	predicted	1000:1008	arg1	sites					1026:1030	all 11 predicted N-glycosylation sites	993:1030	all 11 predicted N-glycosylation sites	993:1030	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	17	37	theme	E2	3051:3052	arg1	protein					3054:3060	the E2 protein	3047:3060	the E2 protein	3047:3060	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	0	38	theme	Virus	75:79	arg1	Proteins					93:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	12	39	theme	E2	2338:2339	arg1	immunogenicity					2320:2333	the immunogenicity	2316:2333	the immunogenicity of E2	2316:2339	Modification of E2 by glycosylation might influence the immunogenicity of E2.					
30651366	5	40	dep	cells	903:907	arg1	ii					886:887	ii	886:887	ii	886:887	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	0	41	theme	Envelope	84:91	arg1	Proteins					93:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	1	42	theme	prophylactic	164:175	arg1	vaccine					177:183	a prophylactic vaccine	162:183	a prophylactic vaccine for hepatitis C virus (HCV)	162:211	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	5	43	theme	E2	770:771	arg1	comparisons					747:757	head-to-head molecular, antigenic, and immunogenic comparisons	696:757	head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	696:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	9	44	link	HEK293-derived	1490:1503	arg1	sE2					1505:1507	HEK293-derived sE2	1490:1507	HEK293-derived sE2 against the homologous HCV H77c isolate	1490:1547	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	6	45	used	utilized	1037:1044	arg2	sites					1026:1030	all 11 predicted N-glycosylation sites	993:1030	all 11 predicted N-glycosylation sites	993:1030	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	5	46	theme	paucimannose-type	930:946	arg1	glycans					948:954	mainly paucimannose-type glycans	923:954	mainly paucimannose-type glycans	923:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	6	47	theme	Mass	957:960	arg1	spectrometry					962:973	Mass spectrometry	957:973	Mass spectrometry	957:973	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	11	48	theme	conserved	2167:2175	arg1	regions					2177:2183	conserved regions	2167:2183	conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies	2167:2261	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	0	49	theme	Mammalian	115:123	arg1	Cells					136:140	Mammalian and Insect Cells	115:140	Mammalian and Insect Cells	115:140	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	9	50	theme	neutralization	1587:1600	arg1	titers					1602:1607	comparable neutralization titers	1576:1607	comparable neutralization titers against heterologous isolates	1576:1637	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	16	51	theme	antibody	2780:2787	arg1	responses					2789:2797	stronger neutralizing antibody responses	2758:2797	stronger neutralizing antibody responses	2758:2797	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	8	52	theme	E2	1373:1374	arg1	E					1368:1368	E	1368:1368	E of E2	1368:1374	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	3	53	theme	cell-based	421:430	arg1	vaccine					432:438	a B cell-based vaccine	417:438	a B cell-based vaccine	417:438	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	11	54	theme	protein	2205:2211	arg1	regions					2177:2183	conserved regions	2167:2183	conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies	2167:2261	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	17	55	theme	glycans	3036:3042	arg1	approach					2966:2973	A more productive approach	2948:2973	A more productive approach for vaccine development	2948:2997	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	17	55	theme	glycans	3036:3042	arg1	deletion					3015:3022	complete deletion	3006:3022	complete deletion of specific glycans	3006:3042	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	14	56	theme	cell	2610:2613	arg1	E2					2615:2616	insect cell E2	2603:2616	insect cell E2	2603:2616	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	16	57	theme	homologous	2811:2820	arg1	isolate					2822:2828	the homologous isolate	2807:2828	the homologous isolate used in the vaccine	2807:2848	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	13	58	theme	immunogenic	2382:2392	arg1	comparisons					2394:2404	molecular and immunogenic comparisons	2368:2404	molecular and immunogenic comparisons of E2 produced in mammalian and insect cells	2368:2449	Accordingly, we performed molecular and immunogenic comparisons of E2 produced in mammalian and insect cells.					
30651366	11	59	theme	capable	2218:2224	arg1	regions					2177:2183	conserved regions	2167:2183	conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies	2167:2261	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	2	60	gly	glycoprotein	336:347	arg1	glycoprotein					336:347	the HCV E2 envelope glycoprotein	316:347	the HCV E2 envelope glycoprotein to facilitate viral clearance	316:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	6	61	from	N-glycans	1092:1100	arg1	sE2					1112:1114	insect sE2	1105:1114	insect sE2	1105:1114	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	0	62	theme	Hepatitis	63:71	arg1	Virus					75:79	Hepatitis C Virus	63:79	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	14	63	theme	glycan	2587:2592	arg1	smaller					2623:2629	smaller	2623:2629	smaller	2623:2629	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	14	63	theme	glycan	2587:2592	arg1	types					2594:2598	the glycan types	2583:2598	the glycan types in insect cell E2	2583:2616	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	6	64	theme	insect	1105:1110	arg1	sE2					1112:1114	insect sE2	1105:1114	insect sE2	1105:1114	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	10	65	theme	specific	1941:1948	arg1	N-glycans					1950:1958	specific N-glycans	1941:1958	specific N-glycans proximal to these epitopes.IMPORTANCE	1941:1996	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	14	66	theme	predicted	2492:2500	arg1	sites					2516:2520	the predicted glycosylation sites	2488:2520	the predicted glycosylation sites	2488:2520	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	2	67	theme	envelope	327:334	arg1	glycoprotein					336:347	the HCV E2 envelope glycoprotein	316:347	the HCV E2 envelope glycoprotein to facilitate viral clearance	316:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	15	68	theme	Mouse	2649:2653	arg1	studies					2670:2676	Mouse immunogenicity studies	2649:2676	Mouse immunogenicity studies	2649:2676	Mouse immunogenicity studies revealed similar polyclonal antibody responses.					
30651366	9	69	theme	neutralizing	1386:1397	arg1	titers					1408:1413	neutralizing antibody titers	1386:1413	neutralizing antibody titers	1386:1413	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	16	70	theme	insect	2735:2740	arg1	E2					2747:2748	insect cell E2	2735:2748	insect cell E2	2735:2748	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	10	71	theme	different	1710:1718	arg1	hosts					1720:1724	different hosts	1710:1724	different hosts	1710:1724	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	9	72	dep	did	1486:1488	arg1	than					1481:1484	than	1481:1484	than	1481:1484	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	6	73	gly	N-glycosylation	1010:1024	arg2	sites					1026:1030	all 11 predicted N-glycosylation sites	993:1030	all 11 predicted N-glycosylation sites	993:1030	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	6	73	gly	N-glycosylation	1010:1024	arg2	11					997:998	11	997:998	11	997:998	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	3	74	theme	neutralizing	518:529	arg1	antibodies					531:540	neutralizing antibodies	518:540	neutralizing antibodies not associated with viral escape	518:573	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	9	75	theme	stronger	1462:1469	arg1	responses					1471:1479	moderately stronger responses	1451:1479	moderately stronger responses	1451:1479	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	1	76	theme	global	223:228	arg1	challenge					237:245	a global health challenge	221:245	a global health challenge	221:245	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	0	77	theme	C	73:73	arg1	Virus					75:79	Hepatitis C Virus	63:79	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	14	78	used	utilized	2527:2534	arg2	sites					2516:2520	the predicted glycosylation sites	2488:2520	the predicted glycosylation sites	2488:2520	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	2	79	theme	viral	363:367	arg1	clearance					369:377	viral clearance	363:377	viral clearance	363:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	14	80	theme	insect	2558:2563	arg1	E2					2570:2571	both mammalian and insect cell E2	2539:2571	both mammalian and insect cell E2	2539:2571	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	16	81	theme	stronger	2758:2765	arg1	responses					2789:2797	stronger neutralizing antibody responses	2758:2797	stronger neutralizing antibody responses	2758:2797	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	10	82	theme	proximal	1960:1967	arg1	N-glycans					1950:1958	specific N-glycans	1941:1958	specific N-glycans proximal to these epitopes.IMPORTANCE	1941:1996	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	14	83	theme	mammalian	2544:2552	arg1	E2					2570:2571	both mammalian and insect cell E2	2539:2571	both mammalian and insect cell E2	2539:2571	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	8	84	theme	antigenic	1345:1353	arg1	domains					1355:1361	antigenic domains	1345:1361	antigenic domains A to E of E2	1345:1374	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	10	85	theme	productive	1800:1809	arg1	approach					1811:1818	a more productive approach	1793:1818	a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge	1793:2087	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	85	theme	productive	1800:1809	arg1	antigenicity					1753:1764	antigenicity	1753:1764	antigenicity	1753:1764	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	1	86	theme	vaccine	177:183	arg1	development					147:157	The development	143:157	The development of a prophylactic vaccine for hepatitis C virus (HCV)	143:211	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	11	87	theme	envelope	2196:2203	arg1	E2					2214:2215	E2	2214:2215	E2	2214:2215	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	11	87	theme	envelope	2196:2203	arg1	protein					2205:2211	the HCV envelope protein	2188:2211	the HCV envelope protein	2188:2211	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	10	88	theme	global	1651:1656	arg1	alteration					1658:1667	global alteration	1651:1667	global alteration of HCV E2 glycosylation by expression in different hosts	1651:1724	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	2	89	theme	HCV	320:322	arg1	glycoprotein					336:347	the HCV E2 envelope glycoprotein	316:347	the HCV E2 envelope glycoprotein to facilitate viral clearance	316:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	15	90	theme	antibody	2706:2713	arg1	responses					2715:2723	similar polyclonal antibody responses	2687:2723	similar polyclonal antibody responses	2687:2723	Mouse immunogenicity studies revealed similar polyclonal antibody responses.					
30651366	1	91	theme	hepatitis	189:197	arg1	HCV					208:210	HCV	208:210	HCV	208:210	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	1	91	theme	hepatitis	189:197	arg1	virus					201:205	hepatitis C virus	189:205	hepatitis C virus (HCV)	189:211	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	16	92	theme	heterologous	2925:2936	arg1	isolates					2938:2945	heterologous isolates	2925:2945	heterologous isolates	2925:2945	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	10	93	theme	hepatitis	2031:2039	arg1	HCV					2050:2052	HCV	2050:2052	HCV	2050:2052	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	93	theme	hepatitis	2031:2039	arg1	virus					2043:2047	hepatitis C virus	2031:2047	hepatitis C virus (HCV)	2031:2053	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	15	94	theme	similar	2687:2693	arg1	responses					2715:2723	similar polyclonal antibody responses	2687:2723	similar polyclonal antibody responses	2687:2723	Mouse immunogenicity studies revealed similar polyclonal antibody responses.					
30651366	11	95	theme	major	2092:2096	arg1	challenge					2098:2106	A major challenge	2090:2106	A major challenge for vaccine development	2090:2130	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	8	96	theme	immunogenicity	1247:1260	arg1	studies					1262:1268	Mouse immunogenicity studies	1241:1268	Mouse immunogenicity studies	1241:1268	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	10	97	theme	E2	1676:1677	arg1	glycosylation					1679:1691	HCV E2 glycosylation	1672:1691	HCV E2 glycosylation	1672:1691	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	98	theme	vaccine	2019:2025	arg1	development					2002:2012	The development	1998:2012	just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV)	1919:2053	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	13	99	theme	E2	2409:2410	arg1	comparisons					2394:2404	molecular and immunogenic comparisons	2368:2404	molecular and immunogenic comparisons of E2 produced in mammalian and insect cells	2368:2449	Accordingly, we performed molecular and immunogenic comparisons of E2 produced in mammalian and insect cells.					
30651366	13	100	theme	molecular	2368:2376	arg1	comparisons					2394:2404	molecular and immunogenic comparisons	2368:2404	molecular and immunogenic comparisons of E2 produced in mammalian and insect cells	2368:2449	Accordingly, we performed molecular and immunogenic comparisons of E2 produced in mammalian and insect cells.					
30651366	10	101	theme	global	2065:2070	arg1	challenge					2079:2087	a global health challenge	2063:2087	a global health challenge	2063:2087	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	5	102	theme	molecular	709:717	arg1	comparisons					747:757	head-to-head molecular, antigenic, and immunogenic comparisons	696:757	head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	696:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	10	103	theme	neutralizing	1859:1870	arg1	epitopes					1872:1879	neutralizing epitopes	1859:1879	neutralizing epitopes	1859:1879	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	5	104	theme	insect	890:895	arg1	cells					903:907	insect (Sf9) cells	890:907	insect (Sf9) cells	890:907	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	11	105	theme	immune	2148:2153	arg1	response					2155:2162	the immune response	2144:2162	the immune response	2144:2162	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	8	106	theme	similar	1284:1290	arg1	responses					1312:1320	similar polyclonal antibody responses	1284:1320	similar polyclonal antibody responses	1284:1320	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	5	107	theme	antigenic	720:728	arg1	comparisons					747:757	head-to-head molecular, antigenic, and immunogenic comparisons	696:757	head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	696:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	9	108	theme	HCV	1532:1534	arg1	isolate					1541:1547	the homologous HCV H77c isolate	1517:1547	the homologous HCV H77c isolate	1517:1547	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	6	109	theme	N-glycosylation	1010:1024	arg1	sites					1026:1030	all 11 predicted N-glycosylation sites	993:1030	all 11 predicted N-glycosylation sites	993:1030	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	8	110	theme	antibody	1303:1310	arg1	responses					1312:1320	similar polyclonal antibody responses	1284:1320	similar polyclonal antibody responses	1284:1320	Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A to E of E2.					
30651366	3	111	theme	conserved	475:483	arg1	epitopes					488:495	conserved E2 epitopes	475:495	conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape	475:573	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	0	112	theme	E2	81:82	arg1	Proteins					93:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	17	113	theme	productive	2955:2964	arg1	approach					2966:2973	A more productive approach	2948:2973	A more productive approach for vaccine development	2948:2997	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	17	113	theme	productive	2955:2964	arg1	deletion					3015:3022	complete deletion	3006:3022	complete deletion of specific glycans	3006:3042	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	10	114	theme	complete	1888:1895	arg1	deletion					1897:1904	complete deletion	1888:1904	complete deletion	1888:1904	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	10	114	theme	complete	1888:1895	arg1	response					1847:1854	the antibody response	1834:1854	the antibody response to neutralizing epitopes	1834:1879	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	0	115	theme	Proteins	93:100	arg1	Immunogenicity					17:30	Immunogenicity	17:30	Immunogenicity	17:30	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	0	115	theme	Proteins	93:100	arg1	Antigenicity					0:11	Antigenicity	0:11	Antigenicity	0:11	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	17	116	from	deletion	3015:3022	arg1	protein					3054:3060	the E2 protein	3047:3060	the E2 protein	3047:3060	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	0	117	theme	Insect	129:134	arg1	Cells					136:140	Mammalian and Insect Cells	115:140	Mammalian and Insect Cells	115:140	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	10	118	dep	approach	1811:1818	arg1	increasing					1823:1832	increasing	1823:1832	to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge	1820:2087	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	5	119	theme	soluble	762:768	arg1	sE2					774:776	sE2	774:776	sE2	774:776	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	5	119	theme	soluble	762:768	arg1	E2					770:771	soluble E2	762:771	soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans	762:954	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	12	120	theme	E2	2280:2281	arg1	Modification					2264:2275	Modification	2264:2275	Modification of E2 by glycosylation	2264:2298	Modification of E2 by glycosylation might influence the immunogenicity of E2.					
30651366	2	121	theme	E2	324:325	arg1	glycoprotein					336:347	the HCV E2 envelope glycoprotein	316:347	the HCV E2 envelope glycoprotein to facilitate viral clearance	316:377	Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance.					
30651366	9	122	theme	comparable	1576:1585	arg1	titers					1602:1607	comparable neutralization titers	1576:1607	comparable neutralization titers against heterologous isolates	1576:1637	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	5	123	theme	Sf9	898:900	arg1	cells					903:907	insect (Sf9) cells	890:907	insect (Sf9) cells	890:907	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	6	124	theme	Sf9-derived	1066:1076	arg1	sE2					1078:1080	Sf9-derived sE2	1066:1080	Sf9-derived sE2	1066:1080	Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex.					
30651366	5	125	theme	HEK293	806:811	arg1	cells					814:818	mammalian (HEK293) cells	795:818	mammalian (HEK293) cells	795:818	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	3	126	theme	significant	391:401	arg1	challenge					403:411	a significant challenge	389:411	a significant challenge for a B cell-based vaccine	389:438	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	10	127	theme	glycosylation	1679:1691	arg1	alteration					1658:1667	global alteration	1651:1667	global alteration of HCV E2 glycosylation by expression in different hosts	1651:1724	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	9	128	theme	heterologous	1617:1628	arg1	isolates					1630:1637	heterologous isolates	1617:1637	heterologous isolates	1617:1637	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	14	129	from	types	2594:2598	arg1	E2					2615:2616	insect cell E2	2603:2616	insect cell E2	2603:2616	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	17	130	theme	vaccine	2979:2985	arg1	development					2987:2997	vaccine development	2979:2997	vaccine development	2979:2997	A more productive approach for vaccine development may be complete deletion of specific glycans in the E2 protein.					
30651366	0	131	theme	Glycosylated	50:61	arg1	Proteins					93:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins	35:100	Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells.					
30651366	14	132	theme	insect	2603:2608	arg1	E2					2615:2616	insect cell E2	2603:2616	insect cell E2	2603:2616	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	13	133	theme	insect	2438:2443	arg1	cells					2445:2449	mammalian and insect cells	2424:2449	mammalian and insect cells	2424:2449	Accordingly, we performed molecular and immunogenic comparisons of E2 produced in mammalian and insect cells.					
30651366	11	134	theme	neutralizing	2239:2250	arg1	antibodies					2252:2261	neutralizing antibodies	2239:2261	neutralizing antibodies	2239:2261	A major challenge for vaccine development is focusing the immune response on conserved regions of the HCV envelope protein, E2, capable of eliciting neutralizing antibodies.					
30651366	3	135	theme	immune	456:461	arg1	response					463:470	the immune response	452:470	the immune response	452:470	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	5	136	theme	mammalian	795:803	arg1	cells					814:818	mammalian (HEK293) cells	795:818	mammalian (HEK293) cells	795:818	Accordingly, we performed head-to-head molecular, antigenic, and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex- and high-mannose-type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose-type glycans.					
30651366	10	137	theme	N-glycans	1950:1958	arg1	modification					1924:1935	just modification	1919:1935	just modification	1919:1935	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	14	138	theme	glycosylation	2502:2514	arg1	sites					2516:2520	the predicted glycosylation sites	2488:2520	the predicted glycosylation sites	2488:2520	Mass spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and insect cell E2, although the glycan types in insect cell E2 were smaller and less complex.					
30651366	3	139	theme	capable	497:503	arg1	epitopes					488:495	conserved E2 epitopes	475:495	conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape	475:573	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	10	140	from	expression	1696:1705	arg1	hosts					1720:1724	different hosts	1710:1724	different hosts	1710:1724	Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes.IMPORTANCE The development of a vaccine for hepatitis C virus (HCV) remains a global health challenge.					
30651366	16	141	theme	cell	2742:2745	arg1	E2					2747:2748	insect cell E2	2735:2748	insect cell E2	2735:2748	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	16	142	theme	comparable	2884:2893	arg1	titers					2910:2915	comparable neutralization titers	2884:2915	comparable neutralization titers against heterologous isolates	2884:2945	However, insect cell E2 induced stronger neutralizing antibody responses against the homologous isolate used in the vaccine, albeit the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	3	143	theme	E2	485:486	arg1	epitopes					488:495	conserved E2 epitopes	475:495	conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape	475:573	However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape.					
30651366	9	144	theme	antibody	1399:1406	arg1	titers					1408:1413	neutralizing antibody titers	1386:1413	neutralizing antibody titers	1386:1413	Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than did HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates.					
30651366	13	145	theme	mammalian	2424:2432	arg1	cells					2445:2449	mammalian and insect cells	2424:2449	mammalian and insect cells	2424:2449	Accordingly, we performed molecular and immunogenic comparisons of E2 produced in mammalian and insect cells.					
30651366	1	146	theme	health	230:235	arg1	challenge					237:245	a global health challenge	221:245	a global health challenge	221:245	The development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge.					
29513734	12	0	theme	future	1929:1934	arg1	studies					1936:1942	especially high antigenicity, future studies	1899:1942	studies	1936:1942	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	7	1	theme	dimer	1075:1079	arg1	formation					1081:1089	dimer formation	1075:1089	dimer formation	1075:1089	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	10	2	theme	three-dimensional	1662:1678	arg1	structure					1680:1688	the overall three-dimensional structure	1650:1688	the overall three-dimensional structure	1650:1688	Although some differences in biochemical properties between thyroid and breast TPO were observed, they do not seem to be critical for the overall three-dimensional structure.					
29513734	10	3	from	differences	1530:1540	arg1	properties					1557:1566	biochemical properties	1545:1566	biochemical properties	1545:1566	Although some differences in biochemical properties between thyroid and breast TPO were observed, they do not seem to be critical for the overall three-dimensional structure.					
29513734	3	4	theme	TPO	411:413	arg1	properties					397:406	the biochemical properties	381:406	the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present	381:528	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	4	5	theme	TPO	629:631	arg1	weight					612:617	the molecular weight	598:617	the molecular weight of breast TPO	598:631	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	4	5	theme	TPO	629:631	arg1	lower					646:650	lower	646:650	lower	646:650	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	3	6	theme	TPO	518:520	arg1	present					522:528	TPO present	518:528	TPO present	518:528	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	5	7	theme	isoelectric	820:830	arg1	point					832:836	isoelectric point	820:836	isoelectric point	820:836	Breast TPO exhibit enzymatic activity and isoelectric point comparable to that of thyroid TPO.					
29513734	3	8	theme	human	544:548	arg1	lines					577:581	human breast tissue-derived cell lines	544:581	human breast tissue-derived cell lines	544:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	5	9	theme	comparable	838:847	arg1	activity					807:814	enzymatic activity	797:814	enzymatic activity	797:814	Breast TPO exhibit enzymatic activity and isoelectric point comparable to that of thyroid TPO.					
29513734	12	10	theme	antigenicity	1915:1926	arg1	studies					1936:1942	especially high antigenicity, future studies	1899:1942	studies	1936:1942	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	6	11	theme	cell	928:931	arg1	lines					933:937	mammary cell lines	920:937	mammary cell lines	920:937	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	4	12	theme	molecular	602:610	arg1	weight					612:617	the molecular weight	598:617	the molecular weight of breast TPO	598:631	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	4	12	theme	molecular	602:610	arg1	lower					646:650	lower	646:650	lower	646:650	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	1	13	theme	thyroid	176:182	arg1	tissues					195:201	thyroid and breast tissues	176:201	thyroid and breast tissues	176:201	Thyroid peroxidase (TPO) is an enzyme and autoantigen expressed in thyroid and breast tissues.					
29513734	8	14	theme	breast	1370:1375	arg1	tissues					1377:1383	breast tissues	1370:1383	breast tissues	1370:1383	Lactoperoxidase, a protein highly homologous to TPO expressed also in breast tissues, does not influence the obtained data.					
29513734	10	15	theme	biochemical	1545:1555	arg1	properties					1557:1566	biochemical properties	1545:1566	biochemical properties	1545:1566	Although some differences in biochemical properties between thyroid and breast TPO were observed, they do not seem to be critical for the overall three-dimensional structure.					
29513734	2	16	theme	Thyroid	204:210	arg1	TPO					212:214	Thyroid TPO	204:214	Thyroid TPO	204:214	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	1	17	theme	breast	188:193	arg1	tissues					195:201	thyroid and breast tissues	176:201	thyroid and breast tissues	176:201	Thyroid peroxidase (TPO) is an enzyme and autoantigen expressed in thyroid and breast tissues.					
29513734	6	18	theme	TPO	903:905	arg1	properties					889:898	The biochemical properties	873:898	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes	873:959	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	6	18	theme	TPO	903:905	arg1	similar					965:971	similar	965:971	similar	965:971	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	3	19	theme	tissue-derived	557:570	arg1	lines					577:581	human breast tissue-derived cell lines	544:581	human breast tissue-derived cell lines	544:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	1	20	theme	Thyroid	109:115	arg1	peroxidase					117:126	Thyroid peroxidase	109:126	Thyroid peroxidase (TPO)	109:132	Thyroid peroxidase (TPO) is an enzyme and autoantigen expressed in thyroid and breast tissues.					
29513734	1	20	theme	Thyroid	109:115	arg1	TPO					129:131	TPO	129:131	TPO	129:131	Thyroid peroxidase (TPO) is an enzyme and autoantigen expressed in thyroid and breast tissues.					
29513734	7	21	theme	enzymatic	1280:1288	arg1	activity					1290:1297	its enzymatic activity	1276:1297	its enzymatic activity	1276:1297	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	2	22	theme	complex	228:234	arg1	process					247:253	a complex maturation process	226:253	a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO	226:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	6	23	theme	glycan	983:988	arg1	content					990:996	glycan content	983:996	glycan content	983:996	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	5	24	theme	Breast	778:783	arg1	TPO					785:787	Breast TPO	778:787	Breast TPO	778:787	Breast TPO exhibit enzymatic activity and isoelectric point comparable to that of thyroid TPO.					
29513734	0	25	theme	human	64:68	arg1	breast					70:75	human breast	64:75	human breast	64:75	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	5	26	theme	thyroid	860:866	arg1	TPO					868:870	thyroid TPO	860:870	thyroid TPO	860:870	Breast TPO exhibit enzymatic activity and isoelectric point comparable to that of thyroid TPO.					
29513734	7	27	theme	TPO	1153:1155	arg1	protein					1157:1163	TPO protein	1153:1163	TPO protein	1153:1163	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	4	28	theme	Western	734:740	arg1	blot					742:745	Western blot	734:745	Western blot also shorter amino acid chain	734:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	0	29	theme	cell	97:100	arg1	lines					102:106	mammary-derived cell lines	81:106	mammary-derived cell lines	81:106	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	11	30	theme	conformation-sensitive	1801:1822	arg1	antibodies					1824:1833	conformation-sensitive antibodies	1801:1833	conformation-sensitive antibodies	1801:1833	This conclusion is supported by the fact that TPO expressed in breast tissues and cell lines reacts well with conformation-sensitive antibodies.					
29513734	0	31	theme	Biochemical	0:10	arg1	properties					12:21	Biochemical properties	0:21	Biochemical properties of thyroid peroxidase (TPO)	0:49	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	6	32	theme	isoelectric	1020:1030	arg1	point					1032:1036	isoelectric point	1020:1036	isoelectric point	1020:1036	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	3	33	theme	human	449:453	arg1	tissues					462:468	normal and cancerous human breast tissues	428:468	normal and cancerous human breast tissues	428:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	7	34	theme	cell	1120:1123	arg1	lines					1125:1129	these cell lines	1114:1129	these cell lines	1114:1129	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	9	35	theme	TPO-specific	1491:1502	arg1	antibodies					1504:1513	TPO-specific antibodies	1491:1513	TPO-specific antibodies	1491:1513	TPO expressed in the cell lines was recognized by a broad panel of TPO-specific antibodies.					
29513734	4	36	theme	chain	771:775	arg1	results					723:729	results	723:729	results of Western blot also shorter amino acid chain	723:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	0	37	theme	peroxidase	34:43	arg1	properties					12:21	Biochemical properties	0:21	Biochemical properties of thyroid peroxidase (TPO)	0:49	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	1	38	dep	enzyme	140:145	arg1	an					137:138	an	137:138	an	137:138	Thyroid peroxidase (TPO) is an enzyme and autoantigen expressed in thyroid and breast tissues.					
29513734	2	39	theme	TPO	340:342	arg1	modifications					306:318	post-translational modifications	287:318	post-translational modifications of breast-expressed TPO	287:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	10	40	dep	thyroid	1576:1582	arg1	TPO					1595:1597	TPO	1595:1597	TPO	1595:1597	Although some differences in biochemical properties between thyroid and breast TPO were observed, they do not seem to be critical for the overall three-dimensional structure.					
29513734	11	41	theme	breast	1754:1759	arg1	tissues					1761:1767	breast tissues	1754:1767	breast tissues	1754:1767	This conclusion is supported by the fact that TPO expressed in breast tissues and cell lines reacts well with conformation-sensitive antibodies.					
29513734	4	42	theme	amino	760:764	arg1	chain					771:775	Western blot also shorter amino acid chain	734:775	Western blot also shorter amino acid chain	734:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	7	43	located	localized	1169:1177	arg1	compartment					1198:1208	the cytoplasmic compartment	1182:1208	the cytoplasmic compartment	1182:1208	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	43	located	localized	1169:1177	arg2	majority					1141:1148	the majority	1137:1148	the majority of TPO protein	1137:1163	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	6	44	theme	biochemical	877:887	arg1	properties					889:898	The biochemical properties	873:898	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes	873:959	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	6	44	theme	biochemical	877:887	arg1	similar					965:971	similar	965:971	similar	965:971	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	0	45	link	mammary-derived	81:95	arg1	lines					102:106	mammary-derived cell lines	81:106	mammary-derived cell lines	81:106	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	3	46	theme	maturation	479:488	arg1	process					490:496	the maturation process	475:496	the maturation process	475:496	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	3	46	theme	maturation	479:488	arg1	TPO					411:413	TPO	411:413	TPO expressed in normal and cancerous human breast tissues	411:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	12	47	theme	potential	1967:1975	arg1	immunotherapies					1977:1991	the potential immunotherapies	1963:1991	the potential immunotherapies directed against breast-expressed TPO and its specific epitopes	1963:2055	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	7	48	theme	cytoplasmic	1186:1196	arg1	compartment					1198:1208	the cytoplasmic compartment	1182:1208	the cytoplasmic compartment	1182:1208	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	3	49	theme	present	522:528	arg1	process					490:496	the maturation process	475:496	the maturation process	475:496	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	3	49	theme	present	522:528	arg1	TPO					411:413	TPO	411:413	TPO expressed in normal and cancerous human breast tissues	411:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	3	49	theme	present	522:528	arg1	antigenicity					502:513	antigenicity	502:513	antigenicity of TPO present	502:528	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	7	50	theme	peroxidase	1051:1060	arg1	activity					1062:1069	no peroxidase activity	1048:1069	no peroxidase activity	1048:1069	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	51	located	detected	1095:1102	arg1	any					1107:1109	any	1107:1109	any	1107:1109	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	51	located	detected	1095:1102	arg2	formation					1081:1089	dimer formation	1075:1089	dimer formation	1075:1089	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	51	located	detected	1095:1102	arg2	activity					1062:1069	no peroxidase activity	1048:1069	no peroxidase activity	1048:1069	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	10	52	theme	overall	1654:1660	arg1	structure					1680:1688	the overall three-dimensional structure	1650:1688	the overall three-dimensional structure	1650:1688	Although some differences in biochemical properties between thyroid and breast TPO were observed, they do not seem to be critical for the overall three-dimensional structure.					
29513734	4	53	theme	breast	622:627	arg1	TPO					629:631	breast TPO	622:631	breast TPO	622:631	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	2	54	theme	post-translational	287:304	arg1	modifications					306:318	post-translational modifications	287:318	post-translational modifications of breast-expressed TPO	287:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	12	55	theme	high	1910:1913	arg1	studies					1936:1942	especially high antigenicity, future studies	1899:1942	studies	1936:1942	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	6	56	theme	mammary	920:926	arg1	lines					933:937	mammary cell lines	920:937	mammary cell lines	920:937	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	11	57	theme	cell	1773:1776	arg1	lines					1778:1782	cell lines	1773:1782	cell lines	1773:1782	This conclusion is supported by the fact that TPO expressed in breast tissues and cell lines reacts well with conformation-sensitive antibodies.					
29513734	3	58	theme	cell	572:575	arg1	lines					577:581	human breast tissue-derived cell lines	544:581	human breast tissue-derived cell lines	544:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	5	59	theme	enzymatic	797:805	arg1	activity					807:814	enzymatic activity	797:814	enzymatic activity	797:814	Breast TPO exhibit enzymatic activity and isoelectric point comparable to that of thyroid TPO.					
29513734	3	60	theme	breast	550:555	arg1	lines					577:581	human breast tissue-derived cell lines	544:581	human breast tissue-derived cell lines	544:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	4	61	theme	thyroid	665:671	arg1	TPO					673:675	thyroid TPO	665:675	thyroid TPO due to decreased glycosylation	665:706	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	2	62	theme	maturation	236:245	arg1	process					247:253	a complex maturation process	226:253	a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO	226:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	7	63	from	surface	1246:1252	arg1	low					1262:1264	low	1262:1264	low	1262:1264	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	63	from	surface	1246:1252	arg1	expression					1223:1232	the TPO expression	1215:1232	the TPO expression at the cell surface	1215:1252	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	8	64	theme	obtained	1409:1416	arg1	data					1418:1421	the obtained data	1405:1421	the obtained data	1405:1421	Lactoperoxidase, a protein highly homologous to TPO expressed also in breast tissues, does not influence the obtained data.					
29513734	12	65	theme	specific	2039:2046	arg1	epitopes					2048:2055	its specific epitopes	2035:2055	its specific epitopes	2035:2055	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	2	66	dep	process	247:253	arg1	known					275:279	known	275:279	is known about post-translational modifications of breast-expressed TPO	272:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	7	67	theme	protein	1157:1163	arg1	majority					1141:1148	the majority	1137:1148	the majority of TPO protein	1137:1163	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	9	68	theme	cell	1445:1448	arg1	lines					1450:1454	the cell lines	1441:1454	the cell lines	1441:1454	TPO expressed in the cell lines was recognized by a broad panel of TPO-specific antibodies.					
29513734	12	69	theme	breast-expressed	2010:2025	arg1	TPO					2027:2029	breast-expressed TPO	2010:2029	breast-expressed TPO	2010:2029	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	0	70	theme	mammary-derived	81:95	arg1	lines					102:106	mammary-derived cell lines	81:106	mammary-derived cell lines	81:106	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	12	71	theme	close	1858:1862	arg1	resemblance					1864:1874	a close resemblance	1856:1874	a close resemblance between both proteins	1856:1896	Taking into account a close resemblance between both proteins, especially high antigenicity, future studies should investigate the potential immunotherapies directed against breast-expressed TPO and its specific epitopes.					
29513734	6	72	theme	normal	943:948	arg1	thyrocytes					950:959	normal thyrocytes	943:959	normal thyrocytes	943:959	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	3	73	theme	normal	428:433	arg1	tissues					462:468	normal and cancerous human breast tissues	428:468	normal and cancerous human breast tissues	428:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	9	74	theme	broad	1476:1480	arg1	panel					1482:1486	a broad panel	1474:1486	a broad panel of TPO-specific antibodies	1474:1513	TPO expressed in the cell lines was recognized by a broad panel of TPO-specific antibodies.					
29513734	4	75	theme	decreased	684:692	arg1	glycosylation					694:706	decreased glycosylation	684:706	decreased glycosylation	684:706	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	0	76	theme	thyroid	26:32	arg1	TPO					46:48	TPO	46:48	TPO	46:48	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	0	76	theme	thyroid	26:32	arg1	peroxidase					34:43	thyroid peroxidase	26:43	thyroid peroxidase (TPO)	26:49	Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.					
29513734	7	77	theme	cell	1241:1244	arg1	surface					1246:1252	the cell surface	1237:1252	the cell surface	1237:1252	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	3	78	theme	breast	455:460	arg1	tissues					462:468	normal and cancerous human breast tissues	428:468	normal and cancerous human breast tissues	428:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	9	79	theme	antibodies	1504:1513	arg1	panel					1482:1486	a broad panel	1474:1486	a broad panel of TPO-specific antibodies	1474:1513	TPO expressed in the cell lines was recognized by a broad panel of TPO-specific antibodies.					
29513734	6	80	theme	molecular	999:1007	arg1	weight					1009:1014	molecular weight	999:1014	molecular weight	999:1014	The biochemical properties of TPO expressed in mammary cell lines and normal thyrocytes are similar regarding glycan content, molecular weight and isoelectric point.					
29513734	3	81	link	tissue-derived	557:570	arg1	lines					577:581	human breast tissue-derived cell lines	544:581	human breast tissue-derived cell lines	544:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	4	82	theme	acid	766:769	arg1	chain					771:775	Western blot also shorter amino acid chain	734:775	Western blot also shorter amino acid chain	734:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	3	83	theme	cancerous	439:447	arg1	tissues					462:468	normal and cancerous human breast tissues	428:468	normal and cancerous human breast tissues	428:468	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	7	84	theme	TPO	1219:1221	arg1	low					1262:1264	low	1262:1264	low	1262:1264	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	7	84	theme	TPO	1219:1221	arg1	expression					1223:1232	the TPO expression	1215:1232	the TPO expression at the cell surface	1215:1252	However, no peroxidase activity and dimer formation was detected in any of these cell lines since the majority of TPO protein was localized in the cytoplasmic compartment, and the TPO expression at the cell surface was too low to detect its enzymatic activity.					
29513734	3	85	theme	biochemical	385:395	arg1	properties					397:406	the biochemical properties	381:406	the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present	381:528	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	8	86	theme	homologous	1334:1343	arg1	Lactoperoxidase					1300:1314	Lactoperoxidase	1300:1314	Lactoperoxidase	1300:1314	Lactoperoxidase, a protein highly homologous to TPO expressed also in breast tissues, does not influence the obtained data.					
29513734	8	86	theme	homologous	1334:1343	arg1	protein					1319:1325	a protein	1317:1325	a protein highly homologous to TPO expressed also in breast tissues	1317:1383	Lactoperoxidase, a protein highly homologous to TPO expressed also in breast tissues, does not influence the obtained data.					
29513734	2	87	theme	breast-expressed	323:338	arg1	TPO					340:342	breast-expressed TPO	323:342	breast-expressed TPO	323:342	Thyroid TPO undergoes a complex maturation process however, nothing is known about post-translational modifications of breast-expressed TPO.					
29513734	3	88	theme	lines	577:581	arg1	panel					535:539	a panel	533:539	a panel of human breast tissue-derived cell lines	533:581	In this study, we have investigated the biochemical properties of TPO expressed in normal and cancerous human breast tissues, and the maturation process and antigenicity of TPO present in a panel of human breast tissue-derived cell lines.					
29513734	4	89	theme	blot	742:745	arg1	chain					771:775	Western blot also shorter amino acid chain	734:775	Western blot also shorter amino acid chain	734:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
29513734	4	90	theme	shorter	752:758	arg1	chain					771:775	Western blot also shorter amino acid chain	734:775	Western blot also shorter amino acid chain	734:775	We found that the molecular weight of breast TPO was slightly lower than that of thyroid TPO due to decreased glycosylation and as suggest results of Western blot also shorter amino acid chain.					
31316527	4	0	theme	predominant	837:847	arg1	glycans					849:855	their predominant glycans	831:855	their predominant glycans	831:855	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	0	theme	predominant	837:847	arg1	N-glycan					810:817	a complex-type N-glycan	795:817	a complex-type N-glycan (GnGnXF)	795:826	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	16	1	theme	protein	2771:2777	arg1	properties					2779:2788	protein properties	2771:2788	protein properties	2771:2788	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	11	2	theme	neutralization	1852:1865	arg1	TNA					1874:1876	TNA	1874:1876	TNA	1874:1876	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	11	2	theme	neutralization	1852:1865	arg1	assay					1867:1871	toxin neutralization assay	1846:1871	toxin neutralization assay (TNA)	1846:1877	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	1	3	theme	cellular	253:260	arg1	level					276:280	the cellular and molecular level	249:280	the cellular and molecular level	249:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	15	4	theme	higher	2668:2673	arg1	tendency					2687:2694	a possibly higher aggregation tendency	2657:2694	a possibly higher aggregation tendency of the ER variant	2657:2712	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	1	5	theme	molecular	266:274	arg1	level					276:280	the cellular and molecular level	249:280	the cellular and molecular level	249:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	14	6	theme	overnight	2369:2377	arg1	incubation					2379:2388	overnight incubation	2369:2388	overnight incubation at 37°C	2369:2396	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	3	7	theme	N-glycosylation	713:727	arg1	site					729:732	the N-glycosylation site	709:732	the N-glycosylation site	709:732	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	15	8	theme	higher	2526:2531	arg1	distance					2533:2540	quantitatively higher distance	2511:2540	quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA)	2511:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	16	9	theme	glycosylation	2831:2843	arg1	effects					2820:2826	the effects	2816:2826	the effects of glycosylation on protein molecular dynamics	2816:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	15	10	theme	Fc	2563:2564	arg1	domains					2566:2572	the CMG2 and Fc domains	2550:2572	domains	2566:2572	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	2	11	theme	N-glycosylation	569:583	arg1	profiles					585:592	N-glycosylation profiles	569:592	N-glycosylation profiles	569:592	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	3	12	theme	point	739:743	arg1	Agly					755:758	Agly	755:758	Agly	755:758	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	3	12	theme	point	739:743	arg1	mutation					745:752	a point mutation	737:752	a point mutation (Agly)	737:759	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	0	13	theme	Decoy	103:107	arg1	Protein					109:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	15	14	theme	MAN8	2487:2490	arg1	glycoform					2492:2500	the MAN8 glycoform	2483:2500	the MAN8 glycoform	2483:2500	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	12	15	theme	binding	2009:2015	arg1	kinetics					2017:2024	The binding kinetics	2005:2024	The binding kinetics between rCMG2-Fc and PA	2005:2048	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	11	16	dep	67.6	1945:1948	arg1	to					1934:1935	to	1934:1935	to	1934:1935	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	12	17	dep	temperatures	2173:2184	arg1	37°C					2194:2197	37°C	2194:2197	37°C	2194:2197	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	12	17	dep	temperatures	2173:2184	arg1	25					2187:2188	25	2187:2188	25	2187:2188	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	15	18	theme	variant	2706:2712	arg1	tendency					2687:2694	a possibly higher aggregation tendency	2657:2694	a possibly higher aggregation tendency of the ER variant	2657:2712	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	1	19	from	influences	198:207	arg1	processes					236:244	biological processes	225:244	biological processes at the cellular and molecular level	225:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	19	from	influences	198:207	arg1	variety					214:220	a variety	212:220	a variety of biological processes at the cellular and molecular level	212:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	16	20	from	effects	2820:2826	arg1	dynamics					2866:2873	protein molecular dynamics	2848:2873	protein molecular dynamics	2848:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	8	21	theme	glycosylation	1420:1432	arg1	effects					1409:1415	the effects	1405:1415	the effects of glycosylation at the molecular level	1405:1455	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	12	22	theme	sub-nanomolar	2108:2120	arg1	affinities					2122:2131	sub-nanomolar affinities	2108:2131	sub-nanomolar affinities regardless of protein glycosylation	2108:2167	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	5	23	theme	higher	888:893	arg1	concentration					895:907	a higher concentration	886:907	a higher concentration of mannose-type N-glycans (50%)	886:939	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	5	23	theme	higher	888:893	arg1	%					938:938	50%	936:938	50%	936:938	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	8	24	theme	dynamics	1268:1275	arg1	simulations					1282:1292	molecular dynamics (MD) simulations	1258:1292	molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1258:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	10	25	theme	Agly	1798:1801	arg1	variant					1803:1809	the Agly variant	1794:1809	the Agly variant	1794:1809	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	14	26	from	37°C	2393:2396	arg1	incubation					2379:2388	overnight incubation	2369:2388	overnight incubation at 37°C	2369:2396	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	7	27	theme	toxin	1147:1151	arg1	efficiency					1168:1177	toxin neutralization efficiency	1147:1177	toxin neutralization efficiency	1147:1177	The protein expression, sequence, N-glycosylation profile, binding kinetics to PA, toxin neutralization efficiency, and thermostability were determined experimentally.					
31316527	1	28	theme	important	148:156	arg1	modification					177:188	an important post-translational modification	145:188	an important post-translational modification	145:188	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	28	theme	important	148:156	arg1	N-glycosylation					126:140	Protein N-glycosylation	118:140	Protein N-glycosylation	118:140	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	9	29	from	variant	1489:1495	arg1	simulated					1514:1522	simulated	1514:1522	simulated	1514:1522	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	9	29	from	variant	1489:1495	arg1	glycoform					1467:1475	The MAN8 glycoform	1458:1475	The MAN8 glycoform from the ER variant	1458:1495	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	6	30	theme	decoy	946:950	arg1	protein					952:958	The decoy protein	942:958	The decoy protein	942:958	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	0	31	from	Effects	0:6	arg1	Stability					59:67	Stability	59:67	Stability	59:67	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	0	31	from	Effects	0:6	arg1	Structure					34:42	Structure	34:42	Structure	34:42	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	0	31	from	Effects	0:6	arg1	Function					45:52	Function	45:52	Function	45:52	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	4	32	contain	had	791:793	arg2	N-glycan					810:817	a complex-type N-glycan	795:817	a complex-type N-glycan (GnGnXF)	795:826	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	32	contain	had	791:793	arg1	variants					782:789	Both the APO and ER variants	762:789	variants	782:789	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	32	contain	had	791:793	arg2	glycans					849:855	their predominant glycans	831:855	their predominant glycans	831:855	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	32	contain	had	791:793	arg2	GnGnXF					820:825	GnGnXF	820:825	GnGnXF	820:825	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	9	33	theme	MAN8	1462:1465	arg1	simulated					1514:1522	simulated	1514:1522	simulated	1514:1522	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	9	33	theme	MAN8	1462:1465	arg1	glycoform					1467:1475	The MAN8 glycoform	1458:1475	The MAN8 glycoform from the ER variant	1458:1495	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	9	34	gly	glycoform	1467:1475	arg1	MAN8					1462:1465	The MAN8 glycoform	1458:1475	The MAN8 glycoform from the ER variant	1458:1495	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	12	35	theme	protein	2147:2153	arg1	glycosylation					2155:2167	protein glycosylation	2147:2167	protein glycosylation	2147:2167	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	0	36	theme	Fc-Fusion	85:93	arg1	Protein					109:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	2	37	theme	decoy	475:479	arg1	protein					481:487	an Fc-fusion anthrax decoy protein	454:487	an Fc-fusion anthrax decoy protein	454:487	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	2	37	theme	decoy	475:479	arg1	rCMG2-Fc					494:501	viz rCMG2-Fc	490:501	viz rCMG2-Fc	490:501	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	1	38	theme	Protein	118:124	arg1	modification					177:188	an important post-translational modification	145:188	an important post-translational modification	145:188	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	38	theme	Protein	118:124	arg1	N-glycosylation					126:140	Protein N-glycosylation	118:140	Protein N-glycosylation	118:140	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	8	39	theme	full-length	1313:1323	arg1	glycoform					1334:1342	the predominant full-length rCMG2-Fc glycoform	1297:1342	the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1297:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	10	40	theme	stabilizing	1608:1618	arg1	effects					1620:1626	strong stabilizing effects	1601:1626	strong stabilizing effects on rCMG2-Fc	1601:1638	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	16	41	theme	protein	2848:2854	arg1	dynamics					2866:2873	protein molecular dynamics	2848:2873	protein molecular dynamics	2848:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	14	42	theme	Agly	2449:2452	arg1	variants					2454:2461	Agly variants	2449:2461	Agly variants	2449:2461	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	9	43	theme	resolve	1527:1533	arg1	differences					1535:1545	resolve differences	1527:1545	resolve differences between the APO and ER variants	1527:1577	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	2	44	theme	Fc-fusion	457:465	arg1	protein					481:487	an Fc-fusion anthrax decoy protein	454:487	an Fc-fusion anthrax decoy protein	454:487	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	2	44	theme	Fc-fusion	457:465	arg1	rCMG2-Fc					494:501	viz rCMG2-Fc	490:501	viz rCMG2-Fc	490:501	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	8	45	theme	glycoform	1334:1342	arg1	simulations					1282:1292	molecular dynamics (MD) simulations	1258:1292	molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1258:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	10	46	theme	planta	1650:1655	arg1	accumulation					1657:1668	planta accumulation	1650:1668	planta accumulation	1650:1668	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	0	47	theme	N-Glycosylation	11:25	arg1	Effects					0:6	Effects	0:6	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein	0:115	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	5	48	theme	N-glycans	925:933	arg1	concentration					895:907	a higher concentration	886:907	a higher concentration of mannose-type N-glycans (50%)	886:939	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	5	48	theme	N-glycans	925:933	arg1	%					938:938	50%	936:938	50%	936:938	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	3	49	gly	N-glycosylation	713:727	arg2	site					729:732	the N-glycosylation site	709:732	the N-glycosylation site	709:732	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	11	50	theme	effective	1885:1893	arg1	concentration					1895:1907	effective concentration	1885:1907	effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER)	1885:2002	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	11	50	theme	effective	1885:1893	arg1	EC50					1910:1913	EC50	1910:1913	EC50	1910:1913	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	13	51	from	information	2284:2294	arg1	differences					2304:2314	EC50 differences	2299:2314	EC50 differences	2299:2314	The protein thermostability was examined utilizing the PA binding ELISA to provide information on EC50 differences.					
31316527	2	52	theme	protein	434:440	arg1	properties					442:451	protein properties	434:451	protein properties	434:451	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	15	53	theme	accessible	2613:2622	arg1	areas					2632:2636	higher hydrophobic solvent accessible surface areas	2586:2636	higher hydrophobic solvent accessible surface areas (SASA)	2586:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	15	53	theme	accessible	2613:2622	arg1	SASA					2639:2642	SASA	2639:2642	SASA	2639:2642	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	9	54	theme	APO	1559:1561	arg1	variants					1570:1577	the APO and ER variants	1555:1577	variants	1570:1577	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	14	55	theme	ER	2344:2345	arg1	variant					2347:2353	functional ER variant	2333:2353	functional ER variant	2333:2353	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	15	56	theme	hydrophobic	2593:2603	arg1	areas					2632:2636	higher hydrophobic solvent accessible surface areas	2586:2636	higher hydrophobic solvent accessible surface areas (SASA)	2586:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	15	56	theme	hydrophobic	2593:2603	arg1	SASA					2639:2642	SASA	2639:2642	SASA	2639:2642	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	1	57	theme	study	312:316	arg1	aspect					318:323	a major study aspect	304:323	a major study aspect for glycoprotein-based therapeutics	304:359	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	9	58	theme	ER	1567:1568	arg1	variants					1570:1577	the APO and ER variants	1555:1577	variants	1570:1577	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	8	59	theme	N-glycosylation	1366:1380	arg1	profiles					1382:1389	the three N-glycosylation profiles	1356:1389	the three N-glycosylation profiles	1356:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	15	60	theme	CMG2	2554:2557	arg1	domains					2566:2572	the CMG2 and Fc domains	2550:2572	domains	2566:2572	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	14	61	theme	significant	2406:2416	arg1	change					2418:2423	no significant change	2403:2423	no significant change	2403:2423	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	13	62	theme	binding	2259:2265	arg1	ELISA					2267:2271	the PA binding ELISA	2252:2271	the PA binding ELISA	2252:2271	The protein thermostability was examined utilizing the PA binding ELISA to provide information on EC50 differences.					
31316527	6	63	theme	toxin	1045:1049	arg1	endocytosis					1051:1061	toxin endocytosis	1045:1061	toxin endocytosis	1045:1061	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	11	64	from	ng/ml	1969:1973	arg1	rankings					1916:1923	effective concentration (EC50) rankings	1885:1923	effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER)	1885:2002	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	10	65	theme	higher	1700:1705	arg1	expression					1707:1716	2-fold higher expression	1693:1716	2-fold higher expression	1693:1716	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	1	66	theme	processes	236:244	arg1	processes					236:244	biological processes	225:244	biological processes at the cellular and molecular level	225:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	66	theme	processes	236:244	arg1	variety					214:220	a variety	212:220	a variety of biological processes at the cellular and molecular level	212:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	15	67	theme	higher	2586:2591	arg1	areas					2632:2636	higher hydrophobic solvent accessible surface areas	2586:2636	higher hydrophobic solvent accessible surface areas (SASA)	2586:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	15	67	theme	higher	2586:2591	arg1	SASA					2639:2642	SASA	2639:2642	SASA	2639:2642	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	11	68	from	ng/ml	1950:1954	arg1	rankings					1916:1923	effective concentration (EC50) rankings	1885:1923	effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER)	1885:2002	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	15	69	theme	aggregation	2675:2685	arg1	tendency					2687:2694	a possibly higher aggregation tendency	2657:2694	a possibly higher aggregation tendency of the ER variant	2657:2712	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	11	70	theme	toxin	1846:1850	arg1	TNA					1874:1876	TNA	1874:1876	TNA	1874:1876	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	11	70	theme	toxin	1846:1850	arg1	assay					1867:1871	toxin neutralization assay	1846:1871	toxin neutralization assay (TNA)	1846:1877	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	6	71	theme	CMG2	1020:1023	arg1	domain					1025:1030	its CMG2 domain	1016:1030	its CMG2 domain	1016:1030	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	10	72	theme	protein	1727:1733	arg1	degradation					1735:1745	less protein degradation	1722:1745	less protein degradation	1722:1745	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	1	73	theme	post-translational	158:175	arg1	modification					177:188	an important post-translational modification	145:188	an important post-translational modification	145:188	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	73	theme	post-translational	158:175	arg1	N-glycosylation					126:140	Protein N-glycosylation	118:140	Protein N-glycosylation	118:140	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	74	from	level	276:280	arg1	processes					236:244	biological processes	225:244	biological processes at the cellular and molecular level	225:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	74	from	level	276:280	arg1	variety					214:220	a variety	212:220	a variety of biological processes at the cellular and molecular level	212:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	10	75	theme	glycosylated	1760:1771	arg1	variants					1773:1780	glycosylated variants	1760:1780	glycosylated variants compared to the Agly variant	1760:1809	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	0	76	theme	Protein	109:115	arg1	Stability					59:67	Stability	59:67	Stability	59:67	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	0	76	theme	Protein	109:115	arg1	Structure					34:42	Structure	34:42	Structure	34:42	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	0	76	theme	Protein	109:115	arg1	Function					45:52	Function	45:52	Function	45:52	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	13	77	theme	EC50	2299:2302	arg1	differences					2304:2314	EC50 differences	2299:2314	EC50 differences	2299:2314	The protein thermostability was examined utilizing the PA binding ELISA to provide information on EC50 differences.					
31316527	5	78	theme	ER	871:872	arg1	variant					874:880	ER variant	871:880	ER variant	871:880	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	9	79	theme	ER	1486:1487	arg1	variant					1489:1495	the ER variant	1482:1495	the ER variant	1482:1495	The MAN8 glycoform from the ER variant was additionally simulated to resolve differences between the APO and ER variants.					
31316527	16	80	theme	N-glycosylation	2752:2766	arg1	impacts					2741:2747	the impacts	2737:2747	the impacts of N-glycosylation on protein properties	2737:2788	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	13	81	theme	protein	2205:2211	arg1	thermostability					2213:2227	The protein thermostability	2201:2227	The protein thermostability	2201:2227	The protein thermostability was examined utilizing the PA binding ELISA to provide information on EC50 differences.					
31316527	11	82	theme	Protein	1812:1818	arg1	function					1820:1827	Protein function	1812:1827	Protein function	1812:1827	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	15	83	theme	ER	2703:2704	arg1	variant					2706:2712	the ER variant	2699:2712	the ER variant	2699:2712	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	1	84	from	variety	214:220	arg1	level					276:280	the cellular and molecular level	249:280	the cellular and molecular level	249:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	5	85	contain	had	882:884	arg2	concentration					895:907	a higher concentration	886:907	a higher concentration of mannose-type N-glycans (50%)	886:939	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	5	85	contain	had	882:884	arg2	%					938:938	50%	936:938	50%	936:938	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	5	85	contain	had	882:884	arg1	variant					874:880	ER variant	871:880	ER variant	871:880	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	3	86	theme	plant	648:652	arg1	reticulum					682:690	endoplasmic reticulum	670:690	endoplasmic reticulum (ER)	670:695	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	3	86	theme	plant	648:652	arg1	APO					664:666	APO	664:666	APO	664:666	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	3	86	theme	plant	648:652	arg1	apoplast					654:661	plant apoplast	648:661	plant apoplast (APO)	648:667	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	6	87	theme	protective	973:982	arg1	PA					993:994	PA	993:994	PA	993:994	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	6	87	theme	protective	973:982	arg1	antigen					984:990	the protective antigen	969:990	the protective antigen (PA) of anthrax	969:1006	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	2	88	theme	profiles	585:592	arg1	types					560:564	three types	554:564	three types of N-glycosylation profiles	554:592	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	8	89	theme	molecular	1441:1449	arg1	level					1451:1455	the molecular level	1437:1455	the molecular level	1437:1455	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	11	90	from	ng/ml	1993:1997	arg1	rankings					1916:1923	effective concentration (EC50) rankings	1885:1923	effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER)	1885:2002	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	7	91	theme	neutralization	1153:1166	arg1	efficiency					1168:1177	toxin neutralization efficiency	1147:1177	toxin neutralization efficiency	1147:1177	The protein expression, sequence, N-glycosylation profile, binding kinetics to PA, toxin neutralization efficiency, and thermostability were determined experimentally.					
31316527	16	92	from	impacts	2741:2747	arg1	properties					2779:2788	protein properties	2771:2788	protein properties	2771:2788	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	8	93	theme	molecular	1258:1266	arg1	dynamics					1268:1275	molecular dynamics	1258:1275	molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1258:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	8	93	theme	molecular	1258:1266	arg1	MD					1278:1279	MD	1278:1279	MD	1278:1279	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	1	94	theme	glycoprotein-based	329:346	arg1	therapeutics					348:359	glycoprotein-based therapeutics	329:359	glycoprotein-based therapeutics	329:359	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	15	95	gly	glycoform	2492:2500	arg1	MAN8					2487:2490	the MAN8 glycoform	2483:2500	the MAN8 glycoform	2483:2500	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	0	96	theme	Plant-Made	74:83	arg1	Protein					109:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	0	97	theme	Anthrax	95:101	arg1	Protein					109:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	a Plant-Made Fc-Fusion Anthrax Decoy Protein	72:115	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	2	98	theme	Nicotiana	521:529	arg1	benthamiana					531:541	Nicotiana benthamiana plant	521:547	Nicotiana benthamiana plant	521:547	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	7	99	theme	binding	1123:1129	arg1	kinetics					1131:1138	binding kinetics	1123:1138	binding kinetics to PA	1123:1144	The protein expression, sequence, N-glycosylation profile, binding kinetics to PA, toxin neutralization efficiency, and thermostability were determined experimentally.					
31316527	16	100	theme	molecular	2856:2864	arg1	dynamics					2866:2873	protein molecular dynamics	2848:2873	protein molecular dynamics	2848:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	6	101	theme	anthrax	1000:1006	arg1	PA					993:994	PA	993:994	PA	993:994	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	6	101	theme	anthrax	1000:1006	arg1	antigen					984:990	the protective antigen	969:990	the protective antigen (PA) of anthrax	969:1006	The decoy protein binds to the protective antigen (PA) of anthrax through its CMG2 domain and inhibits toxin endocytosis.					
31316527	2	102	theme	anthrax	467:473	arg1	protein					481:487	an Fc-fusion anthrax decoy protein	454:487	an Fc-fusion anthrax decoy protein	454:487	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	2	102	theme	anthrax	467:473	arg1	rCMG2-Fc					494:501	viz rCMG2-Fc	490:501	viz rCMG2-Fc	490:501	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	8	103	theme	predominant	1301:1311	arg1	glycoform					1334:1342	the predominant full-length rCMG2-Fc glycoform	1297:1342	the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1297:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	10	104	theme	strong	1601:1606	arg1	effects					1620:1626	strong stabilizing effects	1601:1626	strong stabilizing effects on rCMG2-Fc	1601:1638	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	15	105	theme	surface	2624:2630	arg1	areas					2632:2636	higher hydrophobic solvent accessible surface areas	2586:2636	higher hydrophobic solvent accessible surface areas (SASA)	2586:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	15	105	theme	surface	2624:2630	arg1	SASA					2639:2642	SASA	2639:2642	SASA	2639:2642	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	10	106	gly	glycosylated	1760:1771	arg1	variants					1773:1780	glycosylated variants	1760:1780	glycosylated variants compared to the Agly variant	1760:1809	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	2	107	dep	benthamiana	531:541	arg1	plant					543:547	plant	543:547	Nicotiana benthamiana plant	521:547	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	5	108	theme	mannose-type	912:923	arg1	N-glycans					925:933	mannose-type N-glycans	912:933	mannose-type N-glycans	912:933	In addition, ER variant had a higher concentration of mannose-type N-glycans (50%).					
31316527	1	109	contain	has	194:196	arg2	influences					198:207	influences	198:207	influences on a variety of biological processes at the cellular and molecular level	198:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	109	contain	has	194:196	arg1	N-glycosylation					126:140	Protein N-glycosylation	118:140	Protein N-glycosylation	118:140	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	1	109	contain	has	194:196	arg1	modification					177:188	an important post-translational modification	145:188	an important post-translational modification	145:188	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	8	110	theme	rCMG2-Fc	1325:1332	arg1	glycoform					1334:1342	the predominant full-length rCMG2-Fc glycoform	1297:1342	the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1297:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	2	111	theme	comprehensive	375:387	arg1	understanding					389:401	a comprehensive understanding	373:401	a comprehensive understanding on how N-glycosylation impacts protein properties	373:451	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	15	112	theme	solvent	2605:2611	arg1	areas					2632:2636	higher hydrophobic solvent accessible surface areas	2586:2636	higher hydrophobic solvent accessible surface areas (SASA)	2586:2643	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	15	112	theme	solvent	2605:2611	arg1	SASA					2639:2642	SASA	2639:2642	SASA	2639:2642	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	16	113	gly	glycosylation	2831:2843	arg1	protein					2848:2854	protein molecular dynamics	2848:2873	protein molecular dynamics	2848:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	16	113	gly	glycosylation	2831:2843	arg1	molecular					2856:2864	protein molecular dynamics	2848:2873	protein molecular dynamics	2848:2873	This study highlights the impacts of N-glycosylation on protein properties and provides insight into the effects of glycosylation on protein molecular dynamics.					
31316527	2	114	theme	viz	490:492	arg1	protein					481:487	an Fc-fusion anthrax decoy protein	454:487	an Fc-fusion anthrax decoy protein	454:487	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	2	114	theme	viz	490:492	arg1	rCMG2-Fc					494:501	viz rCMG2-Fc	490:501	viz rCMG2-Fc	490:501	To achieve a comprehensive understanding on how N-glycosylation impacts protein properties, an Fc-fusion anthrax decoy protein, viz rCMG2-Fc, was expressed in Nicotiana benthamiana plant with three types of N-glycosylation profiles.					
31316527	12	115	theme	bio-layer	2069:2077	arg1	interferometry					2079:2092	bio-layer interferometry	2069:2092	bio-layer interferometry (BLI)	2069:2098	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	12	115	theme	bio-layer	2069:2077	arg1	BLI					2095:2097	BLI	2095:2097	BLI	2095:2097	The binding kinetics between rCMG2-Fc and PA were measured with bio-layer interferometry (BLI), giving sub-nanomolar affinities regardless of protein glycosylation and temperatures (25 and 37°C).					
31316527	14	116	theme	variant	2347:2353	arg1	fraction					2321:2328	The fraction	2317:2328	The fraction of functional ER variant	2317:2353	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	11	117	theme	concentration	1895:1907	arg1	rankings					1916:1923	effective concentration (EC50) rankings	1885:1923	effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER)	1885:2002	Protein function was confirmed by toxin neutralization assay (TNA), with effective concentration (EC50) rankings from low to high of 67.6 ng/ml (APO), 83.15 ng/ml (Agly), and 128.9 ng/ml (ER).					
31316527	7	118	theme	protein	1068:1074	arg1	expression					1076:1085	The protein expression	1064:1085	The protein expression	1064:1085	The protein expression, sequence, N-glycosylation profile, binding kinetics to PA, toxin neutralization efficiency, and thermostability were determined experimentally.					
31316527	1	119	theme	major	306:310	arg1	aspect					318:323	a major study aspect	304:323	a major study aspect for glycoprotein-based therapeutics	304:359	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	0	120	dep	Structure	34:42	arg1	the					30:32	the	30:32	the	30:32	Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein.					
31316527	4	121	theme	complex-type	797:808	arg1	glycans					849:855	their predominant glycans	831:855	their predominant glycans	831:855	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	121	theme	complex-type	797:808	arg1	GnGnXF					820:825	GnGnXF	820:825	GnGnXF	820:825	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	4	121	theme	complex-type	797:808	arg1	N-glycan					810:817	a complex-type N-glycan	795:817	a complex-type N-glycan (GnGnXF)	795:826	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	14	122	theme	functional	2333:2342	arg1	variant					2347:2353	functional ER variant	2333:2353	functional ER variant	2333:2353	The fraction of functional ER variant decayed after overnight incubation at 37°C, and no significant change was observed for APO or Agly variants.					
31316527	8	123	gly	glycoform	1334:1342	arg1	rCMG2-Fc					1325:1332	the predominant full-length rCMG2-Fc glycoform	1297:1342	the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles	1297:1389	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
31316527	13	124	theme	PA	2256:2257	arg1	ELISA					2267:2271	the PA binding ELISA	2252:2271	the PA binding ELISA	2252:2271	The protein thermostability was examined utilizing the PA binding ELISA to provide information on EC50 differences.					
31316527	10	125	from	effects	1620:1626	arg1	rCMG2-Fc					1631:1638	rCMG2-Fc	1631:1638	rCMG2-Fc	1631:1638	Glycosylation showed strong stabilizing effects on rCMG2-Fc during in planta accumulation, evidenced by the over 2-fold higher expression and less protein degradation observed for glycosylated variants compared to the Agly variant.					
31316527	3	126	theme	endoplasmic	670:680	arg1	reticulum					682:690	endoplasmic reticulum	670:690	endoplasmic reticulum (ER)	670:695	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	3	126	theme	endoplasmic	670:680	arg1	apoplast					654:661	plant apoplast	648:661	plant apoplast (APO)	648:667	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	3	126	theme	endoplasmic	670:680	arg1	ER					693:694	ER	693:694	ER	693:694	Three variants were produced by targeting protein to plant apoplast (APO), endoplasmic reticulum (ER) or removing the N-glycosylation site by a point mutation (Agly).					
31316527	4	127	theme	APO	771:773	arg1	variants					782:789	Both the APO and ER variants	762:789	variants	782:789	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	15	128	theme	MD	2467:2468	arg1	simulations					2470:2480	MD simulations	2467:2480	MD simulations	2467:2480	In MD simulations, the MAN8 glycoform exhibits quantitatively higher distance between the CMG2 and Fc domains, as well as higher hydrophobic solvent accessible surface areas (SASA), indicating a possibly higher aggregation tendency of the ER variant.					
31316527	1	129	theme	biological	225:234	arg1	processes					236:244	biological processes	225:244	biological processes at the cellular and molecular level	225:280	Protein N-glycosylation is an important post-translational modification and has influences on a variety of biological processes at the cellular and molecular level, making glycosylation a major study aspect for glycoprotein-based therapeutics.					
31316527	4	130	theme	ER	779:780	arg1	variants					782:789	Both the APO and ER variants	762:789	variants	782:789	Both the APO and ER variants had a complex-type N-glycan (GnGnXF) as their predominant glycans.					
31316527	7	131	theme	N-glycosylation	1098:1112	arg1	profile					1114:1120	N-glycosylation profile	1098:1120	N-glycosylation profile	1098:1120	The protein expression, sequence, N-glycosylation profile, binding kinetics to PA, toxin neutralization efficiency, and thermostability were determined experimentally.					
31316527	8	132	from	level	1451:1455	arg1	effects					1409:1415	the effects	1405:1415	the effects of glycosylation at the molecular level	1405:1455	In parallel, we performed molecular dynamics (MD) simulations of the predominant full-length rCMG2-Fc glycoform for each of the three N-glycosylation profiles to understand the effects of glycosylation at the molecular level.					
29858715	5	0	theme	uncommon	633:640	arg1	glycosylation					649:661	a very uncommon N-type glycosylation	626:661	a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva	626:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	2	1	theme	tumor	363:367	arg1	diagnosis					369:377	tumor diagnosis	363:377	tumor diagnosis	363:377	Important biological functions of PIP concentrations have been demonstrated, e.g. in tumor diagnosis and progression.					
29858715	1	2	theme	seminal	262:268	arg1	plasma					270:275	seminal plasma	262:275	seminal plasma	262:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	8	3	theme	first	1092:1096	arg1	time					1098:1101	the first time	1088:1101	the first time on a specific glycoprotein Ley antigens	1088:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	7	4	located	present	971:977	arg1	organs					942:947	most organs	937:947	most organs	937:947	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	7	4	located	present	971:977	arg2	epitopes					954:961	Ley epitopes	950:961	Ley epitopes	950:961	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	7	4	located	present	971:977	arg1	N-glycans					982:990	N-glycans	982:990	N-glycans	982:990	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	1	5	gly	glycoprotein	187:198	arg1	glycoprotein					187:198	a glycoprotein	185:198	a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma	185:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	1	5	gly	glycoprotein	187:198	arg1	protein					168:174	Prolactin-inducible protein	148:174	Prolactin-inducible protein (PIP)	148:180	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	8	6	gly	glycoprotein	1117:1128	arg1	glycoprotein					1117:1128	a specific glycoprotein Ley antigens	1106:1141	a specific glycoprotein Ley antigens	1106:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	4	7	attach	isolated	559:566	arg1	sources					583:589	different sources	573:589	different sources	573:589	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	4	7	attach	isolated	559:566	arg2	PIP					555:557	PIP	555:557	PIP isolated from different sources by LC-MS(/MS) and 1H-NMR	555:614	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	7	8	from	organs	942:947	arg1	present					971:977	present	971:977	present	971:977	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	10	9	theme	higher	1543:1548	arg1	degree					1550:1555	a higher degree	1541:1555	a higher degree of sialylation compared to individuals lacking the SNP	1541:1610	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	10	10	theme	nucleotide	1359:1368	arg1	SNP					1384:1386	SNP	1384:1386	SNP	1384:1386	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	10	10	theme	nucleotide	1359:1368	arg1	polymorphism					1370:1381	a nonsynonymous single nucleotide polymorphism	1336:1381	a nonsynonymous single nucleotide polymorphism (SNP)	1336:1387	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	2	11	theme	Important	278:286	arg1	functions					299:307	Important biological functions	278:307	Important biological functions of PIP concentrations	278:329	Important biological functions of PIP concentrations have been demonstrated, e.g. in tumor diagnosis and progression.					
29858715	8	12	theme	specific	1108:1115	arg1	antigens					1134:1141	a specific glycoprotein Ley antigens	1106:1141	a specific glycoprotein Ley antigens	1106:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	6	13	theme	tetraantennary	839:852	arg1	structures					854:863	tetraantennary structures	839:863	tetraantennary structures	839:863	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	5	14	gly	glycosylation	649:661	arg1	PIP					666:668	PIP	666:668	PIP in healthy individuals from both, seminal fluid and saliva	666:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	6	15	theme	fucosylated	757:767	arg1	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	8	16	theme	Ley	1130:1132	arg1	antigens					1134:1141	a specific glycoprotein Ley antigens	1106:1141	a specific glycoprotein Ley antigens	1106:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	8	17	theme	NMR	1198:1200	arg1	spectroscopy					1202:1213	NMR spectroscopy	1198:1213	NMR spectroscopy	1198:1213	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	10	18	theme	glycan	1518:1523	arg1	structures					1525:1534	glycan structures	1518:1534	glycan structures	1518:1534	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	0	19	theme	glycan	129:134	arg1	structures					136:145	highly-fucosylated glycan structures	110:145	highly-fucosylated glycan structures	110:145	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	2	20	theme	PIP	312:314	arg1	concentrations					316:329	PIP concentrations	312:329	PIP concentrations	312:329	Important biological functions of PIP concentrations have been demonstrated, e.g. in tumor diagnosis and progression.					
29858715	1	21	theme	body	209:212	arg1	secretions					214:223	body secretions	209:223	body secretions from exocrine glands like saliva and seminal plasma	209:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	6	22	gly	fucosylated	757:767	arg1	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	7	23	from	present	971:977	arg1	organs					942:947	most organs	937:947	most organs	937:947	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	1	24	located	found	200:204	arg1	secretions					214:223	body secretions	209:223	body secretions from exocrine glands like saliva and seminal plasma	209:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	1	24	located	found	200:204	arg2	protein					168:174	Prolactin-inducible protein	148:174	Prolactin-inducible protein (PIP)	148:180	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	1	24	located	found	200:204	arg2	glycoprotein					187:198	a glycoprotein	185:198	a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma	185:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	6	25	from	epitopes	813:820	arg1	tri-					830:833	tri-	830:833	tri-	830:833	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	6	25	from	epitopes	813:820	arg1	structures					854:863	tetraantennary structures	839:863	tetraantennary structures	839:863	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	6	25	from	epitopes	813:820	arg1	bi-					825:827	bi-	825:827	bi-	825:827	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	5	26	theme	PIP	666:668	arg1	glycosylation					649:661	a very uncommon N-type glycosylation	626:661	a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva	626:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	27	theme	healthy	673:679	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	7	28	theme	Ley	950:952	arg1	epitopes					954:961	Ley epitopes	950:961	Ley epitopes	950:961	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	1	29	theme	Prolactin-inducible	148:166	arg1	glycoprotein					187:198	a glycoprotein	185:198	a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma	185:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	1	29	theme	Prolactin-inducible	148:166	arg1	PIP					177:179	PIP	177:179	PIP	177:179	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	1	29	theme	Prolactin-inducible	148:166	arg1	protein					168:174	Prolactin-inducible protein	148:174	Prolactin-inducible protein (PIP)	148:180	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	9	30	gly	glycoproteins	1237:1249	arg1	glycoproteins					1237:1249	specific glycoproteins	1228:1249	specific glycoproteins	1228:1249	So far, for specific glycoproteins Ley epitopes had only been reported on O-glycans.					
29858715	4	31	gly	N-glycosylation	536:550	arg1	PIP					555:557	PIP	555:557	PIP isolated from different sources by LC-MS(/MS) and 1H-NMR	555:614	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	1	32	from	glands	239:244	arg1	secretions					214:223	body secretions	209:223	body secretions from exocrine glands like saliva and seminal plasma	209:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	6	33	theme	multiple	791:798	arg1	epitopes					813:820	multiple Lewisy (Ley) epitopes	791:820	multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan	791:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	10	34	contain	have	1513:1516	arg1	individuals					1429:1439	individuals	1429:1439	individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His	1429:1511	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	10	34	contain	have	1513:1516	arg2	structures					1525:1534	glycan structures	1518:1534	glycan structures	1518:1534	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	0	35	theme	LewisY	85:90	arg1	epitopes					92:99	multiple LewisY epitopes	76:99	multiple LewisY epitopes	76:99	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	9	36	theme	specific	1228:1235	arg1	glycoproteins					1237:1249	specific glycoproteins	1228:1249	specific glycoproteins	1228:1249	So far, for specific glycoproteins Ley epitopes had only been reported on O-glycans.					
29858715	9	37	theme	Ley	1251:1253	arg1	epitopes					1255:1262	Ley epitopes	1251:1262	Ley epitopes	1251:1262	So far, for specific glycoproteins Ley epitopes had only been reported on O-glycans.					
29858715	10	38	theme	glycosylation	1393:1405	arg1	pattern					1407:1413	glycosylation pattern	1393:1413	glycosylation pattern	1393:1413	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	0	39	theme	Unusual	0:6	arg1	glycosylation					15:27	Unusual N-type glycosylation	0:27	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.	0:146	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	6	40	contain	carries	734:740	arg1	PIP					730:732	PIP	730:732	PIP	730:732	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	6	40	contain	carries	734:740	arg2	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	4	41	theme	PIP	555:557	arg1	N-glycosylation					536:550	the N-glycosylation	532:550	the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR	532:614	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	10	42	theme	heterozygous	1441:1452	arg1	individuals					1429:1439	individuals	1429:1439	individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His	1429:1511	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	0	43	gly	highly-fucosylated	110:127	arg1	structures					136:145	highly-fucosylated glycan structures	110:145	highly-fucosylated glycan structures	110:145	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	44	theme	salivary	32:39	arg1	PIP					70:72	PIP	70:72	PIP	70:72	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	44	theme	salivary	32:39	arg1	protein					61:67	salivary prolactin-inducible protein	32:67	salivary prolactin-inducible protein (PIP)	32:73	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	45	theme	protein	61:67	arg1	glycosylation					15:27	Unusual N-type glycosylation	0:27	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.	0:146	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	4	46	theme	N-glycosylation	536:550	arg1	analysis					520:527	the analysis	516:527	the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR	516:614	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	3	47	theme	carcinoma	488:496	arg1	chemotherapy					464:475	chemotherapy	464:475	chemotherapy of mammary carcinoma	464:496	PIP quantity has been also found useful to determine the success of chemotherapy of mammary carcinoma.					
29858715	1	48	theme	exocrine	230:237	arg1	glands					239:244	exocrine glands	230:244	exocrine glands like saliva and seminal plasma	230:275	Prolactin-inducible protein (PIP) is a glycoprotein found in body secretions from exocrine glands like saliva and seminal plasma.					
29858715	0	49	dep	glycosylation	15:27	arg1	generate					101:108	generate	101:108	generate highly-fucosylated glycan structures	101:145	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	10	50	theme	exchange	1489:1496	arg1	51Gln					1498:1502	the amino acid exchange 51Gln	1474:1502	the amino acid exchange 51Gln to 51His	1474:1511	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	6	51	theme	fucosyl	889:895	arg1	residues					897:904	up to nine fucosyl residues	878:904	up to nine fucosyl residues on a tetraantennary glycan	878:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	10	52	theme	amino	1478:1482	arg1	51Gln					1498:1502	the amino acid exchange 51Gln	1474:1502	the amino acid exchange 51Gln to 51His	1474:1511	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	5	53	from	both	698:701	arg1	PIP					666:668	PIP	666:668	PIP in healthy individuals from both, seminal fluid and saliva	666:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	53	from	both	698:701	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	10	54	theme	single	1352:1357	arg1	SNP					1384:1386	SNP	1384:1386	SNP	1384:1386	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	10	54	theme	single	1352:1357	arg1	polymorphism					1370:1381	a nonsynonymous single nucleotide polymorphism	1336:1381	a nonsynonymous single nucleotide polymorphism (SNP)	1336:1387	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	5	55	theme	N-type	642:647	arg1	glycosylation					649:661	a very uncommon N-type glycosylation	626:661	a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva	626:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	6	56	theme	Lewisy	800:805	arg1	epitopes					813:820	multiple Lewisy (Ley) epitopes	791:820	multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan	791:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	5	57	from	individuals	681:691	arg1	glycosylation					649:661	a very uncommon N-type glycosylation	626:661	a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva	626:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	6	58	dep	nine	884:887	arg1	to					881:882	to	881:882	to	881:882	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	2	59	theme	biological	288:297	arg1	functions					299:307	Important biological functions	278:307	Important biological functions of PIP concentrations	278:329	Important biological functions of PIP concentrations have been demonstrated, e.g. in tumor diagnosis and progression.					
29858715	6	60	link	N-linked	769:776	arg1	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	6	61	theme	N-linked	769:776	arg1	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	8	62	theme	N-type	1181:1186	arg1	glycan					1188:1193	an N-type glycan	1178:1193	an N-type glycan	1178:1193	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	10	63	theme	nonsynonymous	1338:1350	arg1	SNP					1384:1386	SNP	1384:1386	SNP	1384:1386	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	10	63	theme	nonsynonymous	1338:1350	arg1	polymorphism					1370:1381	a nonsynonymous single nucleotide polymorphism	1336:1381	a nonsynonymous single nucleotide polymorphism (SNP)	1336:1387	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	2	64	theme	concentrations	316:329	arg1	functions					299:307	Important biological functions	278:307	Important biological functions of PIP concentrations	278:329	Important biological functions of PIP concentrations have been demonstrated, e.g. in tumor diagnosis and progression.					
29858715	8	65	theme	glycoprotein	1117:1128	arg1	antigens					1134:1141	a specific glycoprotein Ley antigens	1106:1141	a specific glycoprotein Ley antigens	1106:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	10	66	theme	sialylation	1560:1570	arg1	degree					1550:1555	a higher degree	1541:1555	a higher degree of sialylation compared to individuals lacking the SNP	1541:1610	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	5	67	from	saliva	722:727	arg1	PIP					666:668	PIP	666:668	PIP in healthy individuals from both, seminal fluid and saliva	666:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	67	from	saliva	722:727	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	6	68	theme	tetraantennary	911:924	arg1	glycan					926:931	a tetraantennary glycan	909:931	a tetraantennary glycan	909:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	5	69	from	fluid	712:716	arg1	PIP					666:668	PIP	666:668	PIP in healthy individuals from both, seminal fluid and saliva	666:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	69	from	fluid	712:716	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	0	70	theme	multiple	76:83	arg1	epitopes					92:99	multiple LewisY epitopes	76:99	multiple LewisY epitopes	76:99	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	5	71	theme	seminal	704:710	arg1	fluid					712:716	seminal fluid	704:716	seminal fluid	704:716	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	7	72	theme	most	937:940	arg1	organs					942:947	most organs	937:947	most organs	937:947	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	7	73	attach	present	971:977	arg1	organs					942:947	most organs	937:947	most organs	937:947	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	7	73	attach	present	971:977	arg2	epitopes					954:961	Ley epitopes	950:961	Ley epitopes	950:961	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	7	73	attach	present	971:977	arg1	N-glycans					982:990	N-glycans	982:990	N-glycans	982:990	In most organs, Ley epitopes are not present on N-glycans except in case of a tumor when it is highly up-regulated and important for prognosis.					
29858715	0	74	theme	highly-fucosylated	110:127	arg1	structures					136:145	highly-fucosylated glycan structures	110:145	highly-fucosylated glycan structures	110:145	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	75	theme	N-type	8:13	arg1	glycosylation					15:27	Unusual N-type glycosylation	0:27	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.	0:146	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	3	76	theme	PIP	396:398	arg1	quantity					400:407	PIP quantity	396:407	PIP quantity	396:407	PIP quantity has been also found useful to determine the success of chemotherapy of mammary carcinoma.					
29858715	4	77	theme	different	573:581	arg1	sources					583:589	different sources	573:589	different sources	573:589	Here, we present the analysis of the N-glycosylation of PIP isolated from different sources by LC-MS(/MS) and 1H-NMR.					
29858715	3	78	theme	mammary	480:486	arg1	carcinoma					488:496	mammary carcinoma	480:496	mammary carcinoma	480:496	PIP quantity has been also found useful to determine the success of chemotherapy of mammary carcinoma.					
29858715	0	79	theme	prolactin-inducible	41:59	arg1	PIP					70:72	PIP	70:72	PIP	70:72	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	79	theme	prolactin-inducible	41:59	arg1	protein					61:67	salivary prolactin-inducible protein	32:67	salivary prolactin-inducible protein (PIP)	32:73	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	6	80	theme	unusual	742:748	arg1	glycans					778:784	unusual highly fucosylated N-linked glycans	742:784	unusual highly fucosylated N-linked glycans	742:784	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	3	81	theme	chemotherapy	464:475	arg1	success					453:459	the success	449:459	the success of chemotherapy of mammary carcinoma	449:496	PIP quantity has been also found useful to determine the success of chemotherapy of mammary carcinoma.					
29858715	5	82	from	glycosylation	649:661	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	0	83	gly	glycosylation	15:27	arg1	PIP					70:72	PIP	70:72	PIP	70:72	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	0	83	gly	glycosylation	15:27	arg1	protein					61:67	salivary prolactin-inducible protein	32:67	salivary prolactin-inducible protein (PIP)	32:73	Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple LewisY epitopes generate highly-fucosylated glycan structures.					
29858715	6	84	from	residues	897:904	arg1	glycan					926:931	a tetraantennary glycan	909:931	a tetraantennary glycan	909:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	8	85	from	time	1098:1101	arg1	antigens					1134:1141	a specific glycoprotein Ley antigens	1106:1141	a specific glycoprotein Ley antigens	1106:1141	Here, for the first time on a specific glycoprotein Ley antigens are unambiguously characterized on an N-type glycan by NMR spectroscopy.					
29858715	6	86	theme	Ley	808:810	arg1	epitopes					813:820	multiple Lewisy (Ley) epitopes	791:820	multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan	791:931	PIP carries unusual highly fucosylated N-linked glycans with multiple Lewisy (Ley) epitopes on bi-, tri- and tetraantennary structures resulting in up to nine fucosyl residues on a tetraantennary glycan.					
29858715	10	87	theme	acid	1484:1487	arg1	51Gln					1498:1502	the amino acid exchange 51Gln	1474:1502	the amino acid exchange 51Gln to 51His	1474:1511	Furthermore, a correlation between a nonsynonymous single nucleotide polymorphism (SNP) and glycosylation pattern was detected: individuals heterozygous for the SNP causing the amino acid exchange 51Gln to 51His have glycan structures with a higher degree of sialylation compared to individuals lacking the SNP.					
29858715	5	88	from	PIP	666:668	arg1	saliva					722:727	saliva	722:727	saliva	722:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	88	from	PIP	666:668	arg1	both					698:701	both	698:701	both	698:701	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	88	from	PIP	666:668	arg1	fluid					712:716	seminal fluid	704:716	seminal fluid	704:716	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29858715	5	88	from	PIP	666:668	arg1	individuals					681:691	healthy individuals	673:691	healthy individuals from both, seminal fluid and saliva	673:727	We found a very uncommon N-type glycosylation of PIP in healthy individuals from both, seminal fluid and saliva.					
29557467	6	0	dep	mechanism	1260:1268	arg1	the					1256:1258	the	1256:1258	the	1256:1258	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	0	1	from	approach	8:15	arg1	factors					56:62	the structural and stereoelectronic factors	20:62	the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans	20:122	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	4	2	theme	2H3-E3	844:849	arg1	4C1					853:855	2H3 → 2H3-E3 → 4C1	838:855	2H3 → 2H3-E3 → 4C1	838:855	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	2	3	theme	atomistic	349:357	arg1	details					359:365	atomistic details	349:365	atomistic details	349:365	Our goal was to characterize, with atomistic details, the mechanism of sucrose hydrolysis and the catalytic factors that modulate this reaction.					
29557467	5	4	theme	transfer	1158:1165	arg1	mechanisms					1167:1176	electrostatic and charge transfer mechanisms	1133:1176	mechanisms	1167:1176	Our calculations also revealed that Arg475 and Asp588 play a critical role as TS-stabilizers by electrostatic and charge transfer mechanisms, respectively.					
29557467	4	5	theme	good	924:927	arg1	agreement					929:937	good agreement	924:937	good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage	924:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	6	from	matter	1346:1351	arg1	catalysis					1376:1384	enzyme catalysis	1369:1384	enzyme catalysis	1369:1384	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	4	7	theme	→	842:842	arg1	4C1					853:855	2H3 → 2H3-E3 → 4C1	838:855	2H3 → 2H3-E3 → 4C1	838:855	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	3	8	theme	sucrose	505:511	arg1	hydrolysis					513:522	sucrose hydrolysis	505:522	sucrose hydrolysis	505:522	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	9	theme	bond	1022:1025	arg1	breakage					1027:1034	the glycosidic bond breakage	1007:1034	the glycosidic bond breakage	1007:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	10	theme	specific	1235:1242	arg1	features					1244:1251	the specific features	1231:1251	the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1231:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	3	11	theme	first	537:541	arg1	event					543:547	the first event	533:547	the first event	533:547	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	12	theme	glycosidic	1011:1020	arg1	breakage					1027:1034	the glycosidic bond breakage	1007:1034	the glycosidic bond breakage	1007:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	13	theme	kcal	900:903	arg1	barrier					884:890	the calculated energy barrier	862:890	the calculated energy barrier	862:890	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	13	theme	kcal	900:903	arg1	mol-1					905:909	16.4 kcal mol-1	895:909	16.4 kcal mol-1	895:909	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	3	14	theme	Enzyme	710:715	arg1	intermediate					723:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	15	theme	→	851:851	arg1	4C1					853:855	2H3 → 2H3-E3 → 4C1	838:855	2H3 → 2H3-E3 → 4C1	838:855	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	2	16	theme	sucrose	385:391	arg1	hydrolysis					393:402	sucrose hydrolysis	385:402	sucrose hydrolysis	385:402	Our goal was to characterize, with atomistic details, the mechanism of sucrose hydrolysis and the catalytic factors that modulate this reaction.					
29557467	0	17	theme	Streptococcus	103:115	arg1	mutans					117:122	Streptococcus mutans	103:122	Streptococcus mutans	103:122	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	3	18	theme	Covalent	692:699	arg1	intermediate					723:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	19	theme	experimental	944:955	arg1	evidence					957:964	experimental evidence	944:964	experimental evidence showing a major contribution coming from the glycosidic bond breakage	944:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	20	theme	conformational	745:758	arg1	itinerary					760:768	A novel conformational itinerary	737:768	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism	737:820	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	21	theme	enzyme	1369:1374	arg1	catalysis					1376:1384	enzyme catalysis	1369:1384	enzyme catalysis	1369:1384	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	0	22	theme	QM/MM	2:6	arg1	approach					8:15	A QM/MM approach	0:15	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.	0:123	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	3	23	theme	Glycosyl	701:708	arg1	intermediate					723:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	24	theme	2H3	838:840	arg1	4C1					853:855	2H3 → 2H3-E3 → 4C1	838:855	2H3 → 2H3-E3 → 4C1	838:855	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	3	25	theme	intermediate	723:734	arg1	formation					675:683	the formation	671:683	the formation of the Covalent Glycosyl Enzyme (CGE) intermediate	671:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	6	26	theme	first	1205:1209	arg1	This					1193:1196	This	1193:1196	This	1193:1196	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	6	26	theme	first	1205:1209	arg1	report					1211:1216	the first report	1201:1216	the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1201:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	3	27	theme	glycosidic	568:577	arg1	breakage					584:591	the glycosidic bond breakage	564:591	the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477	564:657	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	5	28	theme	electrostatic	1133:1145	arg1	mechanisms					1167:1176	electrostatic and charge transfer mechanisms	1133:1176	mechanisms	1167:1176	Our calculations also revealed that Arg475 and Asp588 play a critical role as TS-stabilizers by electrostatic and charge transfer mechanisms, respectively.					
29557467	6	29	theme	GTF-SI	1305:1310	arg1	hydrolysis					1312:1321	GTF-SI hydrolysis	1305:1321	GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1305:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	2	30	theme	catalytic	412:420	arg1	factors					422:428	the catalytic factors	408:428	the catalytic factors that modulate this reaction	408:456	Our goal was to characterize, with atomistic details, the mechanism of sucrose hydrolysis and the catalytic factors that modulate this reaction.					
29557467	3	31	theme	CGE	718:720	arg1	intermediate					723:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	the Covalent Glycosyl Enzyme (CGE) intermediate	688:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	32	theme	energy	877:882	arg1	barrier					884:890	the calculated energy barrier	862:890	the calculated energy barrier	862:890	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	32	theme	energy	877:882	arg1	mol-1					905:909	16.4 kcal mol-1	895:909	16.4 kcal mol-1	895:909	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	33	theme	inhibitors	1448:1457	arg1	design					1419:1424	the design	1415:1424	the design of novel and specific inhibitors targeting GTF-SI	1415:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	0	34	theme	structural	24:33	arg1	factors					56:62	the structural and stereoelectronic factors	20:62	the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans	20:122	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	1	35	theme	caries	296:301	arg1	formation					303:311	caries formation	296:311	caries formation	296:311	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	3	36	theme	assisted	593:600	arg1	breakage					584:591	the glycosidic bond breakage	564:591	the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477	564:657	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	6	37	theme	specific	1439:1446	arg1	inhibitors					1448:1457	novel and specific inhibitors	1429:1457	novel and specific inhibitors targeting GTF-SI	1429:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	1	38	theme	formation	303:311	arg1	process					285:291	the process	281:291	the process of caries formation	281:311	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	6	39	theme	residues	1284:1291	arg1	features					1244:1251	the specific features	1231:1251	the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1231:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	4	40	with	agreement	929:937	arg1	evidence					957:964	experimental evidence	944:964	experimental evidence showing a major contribution coming from the glycosidic bond breakage	944:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	2	41	theme	hydrolysis	393:402	arg1	factors					422:428	the catalytic factors	408:428	the catalytic factors that modulate this reaction	408:456	Our goal was to characterize, with atomistic details, the mechanism of sucrose hydrolysis and the catalytic factors that modulate this reaction.					
29557467	2	41	theme	hydrolysis	393:402	arg1	mechanism					372:380	the mechanism	368:380	the mechanism of sucrose hydrolysis	368:402	Our goal was to characterize, with atomistic details, the mechanism of sucrose hydrolysis and the catalytic factors that modulate this reaction.					
29557467	0	42	theme	stereoelectronic	39:54	arg1	factors					56:62	the structural and stereoelectronic factors	20:62	the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans	20:122	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	5	43	theme	critical	1098:1105	arg1	role					1107:1110	a critical role	1096:1110	a critical role	1096:1110	Our calculations also revealed that Arg475 and Asp588 play a critical role as TS-stabilizers by electrostatic and charge transfer mechanisms, respectively.					
29557467	6	44	theme	catalytic	1274:1282	arg1	residues					1284:1291	catalytic residues	1274:1291	catalytic residues	1274:1291	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	3	45	theme	nucleophilic	629:640	arg1	attack					642:647	the nucleophilic attack	625:647	the nucleophilic attack of Asp477	625:657	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	3	46	theme	bond	579:582	arg1	breakage					584:591	the glycosidic bond breakage	564:591	the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477	564:657	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	6	47	theme	mechanism	1260:1268	arg1	features					1244:1251	the specific features	1231:1251	the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1231:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	3	48	theme	concerted	481:489	arg1	mechanism					491:499	a concerted mechanism	479:499	a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate	479:734	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	5	49	theme	charge	1151:1156	arg1	mechanisms					1167:1176	electrostatic and charge transfer mechanisms	1133:1176	mechanisms	1167:1176	Our calculations also revealed that Arg475 and Asp588 play a critical role as TS-stabilizers by electrostatic and charge transfer mechanisms, respectively.					
29557467	0	50	from	mutans	117:122	arg1	GTF-SI					91:96	GTF-SI	91:96	GTF-SI from Streptococcus mutans	91:122	A QM/MM approach on the structural and stereoelectronic factors governing glycosylation by GTF-SI from Streptococcus mutans.					
29557467	4	51	theme	reaction	803:810	arg1	mechanism					812:820	the reaction mechanism	799:820	the reaction mechanism	799:820	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	52	theme	calculated	866:875	arg1	barrier					884:890	the calculated energy barrier	862:890	the calculated energy barrier	862:890	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	4	52	theme	calculated	866:875	arg1	mol-1					905:909	16.4 kcal mol-1	895:909	16.4 kcal mol-1	895:909	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	53	theme	novel	1429:1433	arg1	inhibitors					1448:1457	novel and specific inhibitors	1429:1457	novel and specific inhibitors targeting GTF-SI	1429:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	1	54	theme	retaining	214:222	arg1	enzyme					251:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	4	55	theme	major	976:980	arg1	contribution					982:993	a major contribution	974:993	a major contribution coming from the glycosidic bond breakage	974:1034	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	3	56	theme	Asp477	652:657	arg1	attack					642:647	the nucleophilic attack	625:647	the nucleophilic attack of Asp477	625:657	Our results suggest a concerted mechanism for sucrose hydrolysis in which the first event corresponds to the glycosidic bond breakage assisted by Glu515, followed by the nucleophilic attack of Asp477, leading to the formation of the Covalent Glycosyl Enzyme (CGE) intermediate.					
29557467	4	57	theme	novel	739:743	arg1	itinerary					760:768	A novel conformational itinerary	737:768	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism	737:820	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	6	58	theme	sucrose	1326:1332	arg1	hydrolysis					1312:1321	GTF-SI hydrolysis	1305:1321	GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI	1305:1474	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	1	59	theme	glucosyltransferase	224:242	arg1	enzyme					251:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	4	60	theme	moiety	786:791	arg1	itinerary					760:768	A novel conformational itinerary	737:768	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism	737:820	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	1	61	theme	catalytic	187:195	arg1	mechanism					197:205	the catalytic mechanism	183:205	the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation	183:311	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	1	62	theme	GTF-SI	244:249	arg1	enzyme					251:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	the retaining glucosyltransferase GTF-SI enzyme	210:256	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	6	63	theme	relevance	1356:1364	arg1	sucrose					1326:1332	sucrose	1326:1332	sucrose	1326:1332	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	6	63	theme	relevance	1356:1364	arg1	matter					1346:1351	a matter	1344:1351	a matter of relevance	1344:1364	This is the first report dealing with the specific features of the mechanism and catalytic residues involved in GTF-SI hydrolysis of sucrose, which is a matter of relevance in enzyme catalysis and could be valuable to aid the design of novel and specific inhibitors targeting GTF-SI.					
29557467	4	64	theme	glucosyl	777:784	arg1	moiety					786:791	the glucosyl moiety	773:791	the glucosyl moiety	773:791	A novel conformational itinerary of the glucosyl moiety along the reaction mechanism was identified: 2H3 → 2H3-E3 → 4C1, and the calculated energy barrier is 16.4 kcal mol-1, which is in good agreement with experimental evidence showing a major contribution coming from the glycosidic bond breakage.					
29557467	1	65	theme	QM/MM	139:143	arg1	calculations					145:156	QM/MM calculations	139:156	QM/MM calculations	139:156	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29557467	1	66	theme	enzyme	251:256	arg1	mechanism					197:205	the catalytic mechanism	183:205	the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation	183:311	In this work, QM/MM calculations were employed to examine the catalytic mechanism of the retaining glucosyltransferase GTF-SI enzyme, which participates in the process of caries formation.					
29578688	7	0	theme	hybrid	1703:1708	arg1	structures					1710:1719	certain hybrid structures	1695:1719	certain hybrid structures	1695:1719	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	5	1	gly	glycoproteins	1284:1296	arg1	glycoproteins					1284:1296	HIV surface glycoproteins	1272:1296	HIV surface glycoproteins	1272:1296	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	1	2	theme	gp120	264:268	arg1	glycoproteins					289:301	gp120 and gp 41 envelope glycoproteins	264:301	gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	264:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	3	with	PG9	981:983	arg1	avidity					995:1001	high avidity	990:1001	high avidity	990:1001	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	4	theme	complex-type	1066:1077	arg1	arm					1079:1081	complex-type arm	1066:1081	complex-type arm	1066:1081	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	3	5	theme	PG9	691:693	arg1	studies					671:677	Recent epitope mapping studies	648:677	Recent epitope mapping studies of the bNAb PG9	648:693	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	2	6	theme	viral	562:566	arg1	glycans					576:582	the viral surface glycans	558:582	the viral surface glycans	558:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	6	7	theme	potent	1472:1477	arg1	bNAbs					1479:1483	several other highly potent bNAbs	1451:1483	several other highly potent bNAbs	1451:1483	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	6	7	theme	potent	1472:1477	arg1	PG16					1496:1499	PG16	1496:1499	PG16	1496:1499	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	1	8	theme	genetic	181:187	arg1	diversity					189:197	genetic diversity	181:197	genetic diversity	181:197	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	9	theme	sialyl	875:880	arg1	glycan					894:899	sialyl biantennary glycan	875:899	sialyl biantennary glycan	875:899	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	7	10	theme	strong	1659:1664	arg1	avidity					1666:1672	strong avidity	1659:1672	strong avidity (subnanomolar Kd)	1659:1690	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	7	10	theme	strong	1659:1664	arg1	Kd					1688:1689	subnanomolar Kd	1675:1689	subnanomolar Kd	1675:1689	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	6	11	theme	other	1459:1463	arg1	bNAbs					1479:1483	several other highly potent bNAbs	1451:1483	several other highly potent bNAbs	1451:1483	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	6	11	theme	other	1459:1463	arg1	PG16					1496:1499	PG16	1496:1499	PG16	1496:1499	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	4	12	theme	glycan	894:899	arg1	mixture					855:861	a 1:1 mixture	849:861	a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer	849:957	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	1	13	theme	virus	206:210	arg1	diversity					251:259	the substantial sequence diversity	226:259	the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	226:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	13	theme	virus	206:210	arg1	mutability					166:175	extraordinary mutability	152:175	extraordinary mutability	152:175	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	13	theme	virus	206:210	arg1	diversity					189:197	genetic diversity	181:197	genetic diversity	181:197	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	2	14	theme	surface	568:574	arg1	glycans					576:582	the viral surface glycans	558:582	the viral surface glycans	558:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	4	15	theme	PG9	1113:1115	arg1	ligand					1103:1108	the proper ligand	1092:1108	the proper ligand of PG9	1092:1115	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	15	theme	PG9	1113:1115	arg1	glycan					1037:1042	a hybrid glycan	1028:1042	a hybrid glycan with oligomannose and complex-type arm	1028:1081	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	3	16	theme	high	740:743	arg1	mannose					745:751	a properly spaced high mannose	722:751	a properly spaced high mannose	722:751	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	1	17	theme	HIV	115:117	arg1	vaccine					119:125	an HIV vaccine	112:125	an HIV vaccine	112:125	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	18	theme	Man5	866:869	arg1	mixture					855:861	a 1:1 mixture	849:861	a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer	849:957	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	5	19	theme	hybrid-type	1220:1230	arg1	structures					1241:1250	unusual hybrid-type N-glycan structures	1212:1250	unusual hybrid-type N-glycan structures	1212:1250	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	3	20	theme	glycan	772:777	arg1	requirement					707:717	a requirement	705:717	a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer	705:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	5	21	theme	structures	1241:1250	arg1	series					1202:1207	a series	1200:1207	a series	1200:1207	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	3	22	theme	complex	759:765	arg1	glycan					772:777	a complex type glycan	757:777	a complex type glycan connected by a short peptide spacer	757:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	3	23	theme	short	794:798	arg1	spacer					808:813	a short peptide spacer	792:813	a short peptide spacer	792:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	0	24	theme	Neutralizing	68:79	arg1	Antibodies					81:90	Neutralizing Antibodies	68:90	Neutralizing Antibodies	68:90	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1 Broadly Neutralizing Antibodies.					
29578688	1	25	theme	HIV	330:332	arg1	variants					334:341	more than 2000 HIV variants	315:341	more than 2000 HIV variants	315:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	26	theme	well-defined	906:917	arg1	distance					919:926	well-defined distance	906:926	well-defined distance	906:926	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	2	27	theme	antibodies	511:520	arg1	discovery					463:471	the discovery	459:471	the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans	459:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	5	28	theme	HIV	1272:1274	arg1	glycoproteins					1284:1296	HIV surface glycoproteins	1272:1296	HIV surface glycoproteins	1272:1296	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	0	29	theme	Unprecedented	0:12	arg1	Role					14:17	Unprecedented Role	0:17	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1	0:58	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1 Broadly Neutralizing Antibodies.					
29578688	7	30	theme	vaccines	1800:1807	arg1	design					1790:1795	the design	1786:1795	the design of vaccines against HIV	1786:1819	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	2	31	theme	silent	428:433	arg1	self-antigens					435:447	immunologically silent self-antigens	412:447	immunologically silent self-antigens	412:447	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	2	31	theme	silent	428:433	arg1	glycans					363:369	The highly diverse glycans	344:369	The highly diverse glycans on HIV spikes	344:383	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	4	32	with	mixture	855:861	arg1	distance					919:926	well-defined distance	906:926	well-defined distance	906:926	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	0	33	theme	N-Glycans	29:37	arg1	Role					14:17	Unprecedented Role	0:17	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1	0:58	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1 Broadly Neutralizing Antibodies.					
29578688	1	34	theme	glycoproteins	289:301	arg1	diversity					251:259	the substantial sequence diversity	226:259	the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	226:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	34	theme	glycoproteins	289:301	arg1	mutability					166:175	extraordinary mutability	152:175	extraordinary mutability	152:175	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	6	35	theme	hybrid	1361:1366	arg1	glycans					1368:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	6	36	used	used	1386:1389	arg2	glycans					1368:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	3	37	theme	mapping	663:669	arg1	studies					671:677	Recent epitope mapping studies	648:677	Recent epitope mapping studies of the bNAb PG9	648:693	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	6	38	theme	PG9	1443:1445	arg1	studies					1432:1438	the binding studies	1420:1438	the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074	1420:1591	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	4	39	with	glycan	1037:1042	arg1	oligomannose					1049:1060	oligomannose	1049:1060	oligomannose	1049:1060	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	39	with	glycan	1037:1042	arg1	arm					1079:1081	complex-type arm	1066:1081	complex-type arm	1066:1081	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	40	theme	hybrid	1030:1035	arg1	ligand					1103:1108	the proper ligand	1092:1108	the proper ligand of PG9	1092:1115	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	40	theme	hybrid	1030:1035	arg1	glycan					1037:1042	a hybrid glycan	1028:1042	a hybrid glycan with oligomannose and complex-type arm	1028:1081	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	3	41	theme	Recent	648:653	arg1	studies					671:677	Recent epitope mapping studies	648:677	Recent epitope mapping studies of the bNAb PG9	648:693	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	2	42	theme	diverse	355:361	arg1	self-antigens					435:447	immunologically silent self-antigens	412:447	immunologically silent self-antigens	412:447	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	2	42	theme	diverse	355:361	arg1	glycans					363:369	The highly diverse glycans	344:369	The highly diverse glycans on HIV spikes	344:383	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	7	43	theme	certain	1695:1701	arg1	structures					1710:1719	certain hybrid structures	1695:1719	certain hybrid structures	1695:1719	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	3	44	theme	bNAb	686:689	arg1	PG9					691:693	the bNAb PG9	682:693	the bNAb PG9	682:693	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	5	45	theme	N-glycosylation	1322:1336	arg1	pathway					1338:1344	the host-guided N-glycosylation pathway	1306:1344	the host-guided N-glycosylation pathway	1306:1344	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	1	46	theme	sequence	242:249	arg1	diversity					251:259	the substantial sequence diversity	226:259	the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	226:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	46	theme	sequence	242:249	arg1	mutability					166:175	extraordinary mutability	152:175	extraordinary mutability	152:175	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	2	47	from	glycans	363:369	arg1	spikes					378:383	HIV spikes	374:383	HIV spikes	374:383	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	7	48	theme	subnanomolar	1675:1686	arg1	avidity					1666:1672	strong avidity	1659:1672	strong avidity (subnanomolar Kd)	1659:1690	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	7	48	theme	subnanomolar	1675:1686	arg1	Kd					1688:1689	subnanomolar Kd	1675:1689	subnanomolar Kd	1675:1689	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	1	49	theme	extraordinary	152:164	arg1	diversity					251:259	the substantial sequence diversity	226:259	the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	226:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	49	theme	extraordinary	152:164	arg1	mutability					166:175	extraordinary mutability	152:175	extraordinary mutability	152:175	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	6	50	theme	bNAbs	1479:1483	arg1	studies					1432:1438	the binding studies	1420:1438	the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074	1420:1591	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	2	51	theme	major	597:601	arg1	question					603:610	a major question	595:610	a major question about the origin of their antigens	595:645	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	3	52	theme	spaced	733:738	arg1	mannose					745:751	a properly spaced high mannose	722:751	a properly spaced high mannose	722:751	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	2	53	theme	HIV	535:537	arg1	patients					539:546	HIV patients	535:546	HIV patients targeting the viral surface glycans	535:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	6	54	theme	several	1451:1457	arg1	bNAbs					1479:1483	several other highly potent bNAbs	1451:1483	several other highly potent bNAbs	1451:1483	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	6	54	theme	several	1451:1457	arg1	PG16					1496:1499	PG16	1496:1499	PG16	1496:1499	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	4	55	theme	biantennary	882:892	arg1	glycan					894:899	sialyl biantennary glycan	875:899	sialyl biantennary glycan	875:899	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	56	theme	1:1	851:853	arg1	mixture					855:861	a 1:1 mixture	849:861	a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer	849:957	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	5	57	theme	unusual	1212:1218	arg1	structures					1241:1250	unusual hybrid-type N-glycan structures	1212:1250	unusual hybrid-type N-glycan structures	1212:1250	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	3	58	theme	mannose	745:751	arg1	requirement					707:717	a requirement	705:717	a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer	705:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	1	59	theme	vaccine	119:125	arg1	development					97:107	The development	93:107	The development of an HIV vaccine	93:125	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	60	theme	proper	1096:1101	arg1	ligand					1103:1108	the proper ligand	1092:1108	the proper ligand of PG9	1092:1115	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	60	theme	proper	1096:1101	arg1	glycan					1037:1042	a hybrid glycan	1028:1042	a hybrid glycan with oligomannose and complex-type arm	1028:1081	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	5	61	theme	N-glycan	1232:1239	arg1	structures					1241:1250	unusual hybrid-type N-glycan structures	1212:1250	unusual hybrid-type N-glycan structures	1212:1250	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	6	62	theme	synthetic	1351:1359	arg1	glycans					1368:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans	1347:1374	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	1	63	gly	glycoproteins	289:301	arg1	glycoproteins					289:301	gp120 and gp 41 envelope glycoproteins	264:301	gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	264:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	7	64	theme	other	1637:1641	arg1	bNAbs					1643:1647	some other bNAbs	1632:1647	some other bNAbs	1632:1647	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	2	65	theme	potent	483:488	arg1	antibodies					511:520	highly potent broadly neutralizing antibodies	476:520	highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans	476:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	2	65	theme	potent	483:488	arg1	bNAbs					523:527	bNAbs	523:527	bNAbs	523:527	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	3	66	theme	type	767:770	arg1	glycan					772:777	a complex type glycan	757:777	a complex type glycan connected by a short peptide spacer	757:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	2	67	dep	potent	483:488	arg1	neutralizing					498:509	neutralizing	498:509	neutralizing	498:509	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	4	68	theme	peptide	944:950	arg1	spacer					952:957	the peptide spacer	940:957	the peptide spacer	940:957	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	2	69	theme	antigens	638:645	arg1	origin					622:627	the origin	618:627	the origin of their antigens	618:645	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	3	70	theme	peptide	800:806	arg1	spacer					808:813	a short peptide spacer	792:813	a short peptide spacer	792:813	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	2	71	from	discovery	463:471	arg1	patients					539:546	HIV patients	535:546	HIV patients targeting the viral surface glycans	535:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	5	72	theme	surface	1276:1282	arg1	glycoproteins					1284:1296	HIV surface glycoproteins	1272:1296	HIV surface glycoproteins	1272:1296	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	2	73	theme	HIV	374:376	arg1	spikes					378:383	HIV spikes	374:383	HIV spikes	374:383	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	6	74	theme	glycan	1402:1407	arg1	arrays					1409:1414	glycan arrays	1402:1414	glycan arrays	1402:1414	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	0	75	theme	Hybrid	22:27	arg1	N-Glycans					29:37	Hybrid N-Glycans	22:37	Hybrid N-Glycans	22:37	Unprecedented Role of Hybrid N-Glycans as Ligands for HIV-1 Broadly Neutralizing Antibodies.					
29578688	1	76	theme	gp	274:275	arg1	glycoproteins					289:301	gp120 and gp 41 envelope glycoproteins	264:301	gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	264:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	4	77	dep	proposed	1014:1021	arg1	recognized					967:976	recognized	967:976	is well recognized by PG9 with high avidity	959:1001	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	4	78	theme	high	990:993	arg1	avidity					995:1001	high avidity	990:1001	high avidity	990:1001	We have recently discovered that a 1:1 mixture of Man5 and sialyl biantennary glycan with well-defined distance and without the peptide spacer is well recognized by PG9 with high avidity and, thus, proposed that a hybrid glycan with oligomannose and complex-type arm could be the proper ligand of PG9.					
29578688	1	79	theme	envelope	280:287	arg1	glycoproteins					289:301	gp120 and gp 41 envelope glycoproteins	264:301	gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	264:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	7	80	theme	unusual	1744:1750	arg1	glycans					1752:1758	these unusual glycans	1738:1758	these unusual glycans	1738:1758	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	7	80	theme	unusual	1744:1750	arg1	epitopes					1773:1780	epitopes	1773:1780	epitopes for the design of vaccines against HIV	1773:1819	Our results demonstrated that PG9 and some other bNAbs bind with strong avidity (subnanomolar Kd) to certain hybrid structures, suggesting that these unusual glycans may serve as epitopes for the design of vaccines against HIV.					
29578688	3	81	theme	epitope	655:661	arg1	studies					671:677	Recent epitope mapping studies	648:677	Recent epitope mapping studies of the bNAb PG9	648:693	Recent epitope mapping studies of the bNAb PG9 indicated a requirement of a properly spaced high mannose and a complex type glycan connected by a short peptide spacer.					
29578688	1	82	dep	mutability	166:175	arg1	the					148:150	the	148:150	the	148:150	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	2	83	from	patients	539:546	arg1	antibodies					511:520	highly potent broadly neutralizing antibodies	476:520	highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans	476:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	2	83	from	patients	539:546	arg1	bNAbs					523:527	bNAbs	523:527	bNAbs	523:527	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	2	83	from	patients	539:546	arg1	discovery					463:471	the discovery	459:471	the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans	459:582	The highly diverse glycans on HIV spikes are commonly considered as immunologically silent self-antigens; however, the discovery of highly potent broadly neutralizing antibodies (bNAbs) from HIV patients targeting the viral surface glycans has raised a major question about the origin of their antigens.					
29578688	5	84	theme	host-guided	1310:1320	arg1	pathway					1338:1344	the host-guided N-glycosylation pathway	1306:1344	the host-guided N-glycosylation pathway	1306:1344	To verify this proposition, we first designed and chemo-enzymatically synthesized a series of unusual hybrid-type N-glycan structures, which may exist on HIV surface glycoproteins through the host-guided N-glycosylation pathway.					
29578688	6	85	theme	binding	1424:1430	arg1	studies					1432:1438	the binding studies	1420:1438	the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074	1420:1591	The synthetic hybrid glycans were then used to prepare glycan arrays for the binding studies of PG9 and several other highly potent bNAbs, including PG16, PGT121, PGT128-3C, 2G12, VRC13, VRC-PG05, VRC26.25, VRC26.09, PGDM1400, 35O22, and 10-1074.					
29578688	1	86	theme	substantial	230:240	arg1	diversity					251:259	the substantial sequence diversity	226:259	the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants	226:341	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
29578688	1	86	theme	substantial	230:240	arg1	mutability					166:175	extraordinary mutability	152:175	extraordinary mutability	152:175	The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity of the virus, particularly the substantial sequence diversity of gp120 and gp 41 envelope glycoproteins existing in more than 2000 HIV variants.					
31602612	3	0	from	glycoproteins	466:478	arg1	structure					408:416	structure	408:416	structure	408:416	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	0	from	glycoproteins	466:478	arg1	N-glycans					435:443	N-glycans	435:443	N-glycans from yeast cell wall glycoproteins	435:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	0	from	glycoproteins	466:478	arg1	occupancy					422:430	occupancy	422:430	occupancy	422:430	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	1	theme	data-independent	341:356	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	2	theme	mass	378:381	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	3	theme	spectrometry	383:394	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	4	gly	occupancy	422:430	arg2	N-glycans					435:443	N-glycans	435:443	N-glycans from yeast cell wall glycoproteins	435:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	1	5	theme	complex	75:81	arg1	critical					122:129	critical	122:129	critical	122:129	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	1	5	theme	complex	75:81	arg1	modification					101:112	a complex posttranslational modification	73:112	a complex posttranslational modification that is critical for regulating the functions of diverse proteins	73:178	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	1	5	theme	complex	75:81	arg1	Glycosylation					56:68	Glycosylation	56:68	Glycosylation	56:68	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	2	6	from	degree	247:252	arg1	structure					300:308	structure	300:308	structure	300:308	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	2	6	from	degree	247:252	arg1	occupancy					286:294	glycan occupancy	279:294	glycan occupancy	279:294	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	3	7	theme	SWATH	371:375	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	1	8	theme	posttranslational	83:99	arg1	critical					122:129	critical	122:129	critical	122:129	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	1	8	theme	posttranslational	83:99	arg1	modification					101:112	a complex posttranslational modification	73:112	a complex posttranslational modification that is critical for regulating the functions of diverse proteins	73:178	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	1	8	theme	posttranslational	83:99	arg1	Glycosylation					56:68	Glycosylation	56:68	Glycosylation	56:68	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	3	9	theme	wall	461:464	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	10	from	structure	408:416	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	11	theme	structure	408:416	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	12	theme	yeast	450:454	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	0	13	theme	Data-Independent	0:15	arg1	Acquisition					17:27	Data-Independent Acquisition	0:27	Data-Independent Acquisition	0:27	Data-Independent Acquisition for Yeast Glycoproteomics.					
31602612	3	14	theme	cell	456:459	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	0	15	theme	Yeast	33:37	arg1	Glycoproteomics					39:53	Yeast Glycoproteomics	33:53	Yeast Glycoproteomics	33:53	Data-Independent Acquisition for Yeast Glycoproteomics.					
31602612	3	16	theme	N-glycans	435:443	arg1	structure					408:416	structure	408:416	structure	408:416	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	3	16	theme	N-glycans	435:443	arg1	occupancy					422:430	occupancy	422:430	occupancy	422:430	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	2	17	theme	glycosylation	201:213	arg1	Analysis					181:188	Analysis	181:188	Analysis of protein glycosylation	181:213	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	2	18	theme	heterogeneity	257:269	arg1	degree					247:252	the high degree	238:252	the high degree of heterogeneity in both glycan occupancy and structure	238:308	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	2	19	theme	protein	193:199	arg1	glycosylation					201:213	protein glycosylation	193:213	protein glycosylation	193:213	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	3	20	theme	occupancy	422:430	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	2	21	theme	high	242:245	arg1	degree					247:252	the high degree	238:252	the high degree of heterogeneity in both glycan occupancy and structure	238:308	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	3	22	gly	glycoproteins	466:478	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	2	23	theme	glycan	279:284	arg1	occupancy					286:294	glycan occupancy	279:294	glycan occupancy	279:294	Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure.					
31602612	3	24	theme	acquisition	358:368	arg1	analysis					396:403	data-independent acquisition (SWATH) mass spectrometry analysis	341:403	data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins	341:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	0	25	dep	Acquisition	17:27	arg1	Glycoproteomics					39:53	Yeast Glycoproteomics	33:53	Yeast Glycoproteomics	33:53	Data-Independent Acquisition for Yeast Glycoproteomics.					
31602612	1	26	theme	diverse	163:169	arg1	proteins					171:178	diverse proteins	163:178	diverse proteins	163:178	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
31602612	3	27	from	occupancy	422:430	arg1	glycoproteins					466:478	yeast cell wall glycoproteins	450:478	yeast cell wall glycoproteins	450:478	Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.					
31602612	1	28	theme	proteins	171:178	arg1	functions					150:158	the functions	146:158	the functions of diverse proteins	146:178	Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins.					
30344053	0	0	theme	Leukocyte	94:102	arg1	Adhesion					104:111	Selectin Dependent Leukocyte Adhesion	75:111	Selectin Dependent Leukocyte Adhesion	75:111	Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion.					
30344053	4	1	theme	cell	556:559	arg1	surface					561:567	cell surface N- and O-linked glycan biosynthesis	556:603	cell surface N- and O-linked glycan biosynthesis	556:603	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	6	2	theme	lactosamine	822:832	arg1	chains					834:839	lactosamine chains	822:839	lactosamine chains	822:839	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	1	3	theme	biosynthetic	176:187	arg1	acceptors					189:197	naturally occurring biosynthetic acceptors	156:197	naturally occurring biosynthetic acceptors	156:197	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	7	4	theme	prior	1029:1033	arg1	thioglycosides					1014:1027	thioglycosides	1014:1027	thioglycosides prior to infusion into mouse	1014:1056	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	0	5	theme	Dependent	84:92	arg1	Adhesion					104:111	Selectin Dependent Leukocyte Adhesion	75:111	Selectin Dependent Leukocyte Adhesion	75:111	Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion.					
30344053	9	6	theme	inflammatory	1301:1312	arg1	accumulation					1324:1335	inflammatory leukocyte accumulation	1301:1335	inflammatory leukocyte accumulation	1301:1335	They reduce N-/O-linked glycan biosynthesis and inflammatory leukocyte accumulation.					
30344053	7	7	with	Treatment	983:991	arg1	thioglycosides					1014:1027	thioglycosides	1014:1027	thioglycosides prior to infusion into mouse	1014:1056	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	6	8	theme	inflamed	937:944	arg1	cells					958:962	inflamed endothelial cells	937:962	inflamed endothelial cells under fluid shear	937:980	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	1	9	theme	acceptors	189:197	arg1	analogs					145:151	synthetic analogs	135:151	synthetic analogs of naturally occurring biosynthetic acceptors	135:197	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	1	9	theme	acceptors	189:197	arg1	decoys					124:129	Metabolic decoys	114:129	Metabolic decoys	114:129	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	7	10	theme	granulocytes	996:1007	arg1	Treatment					983:991	Treatment	983:991	Treatment of granulocytes with thioglycosides prior to infusion into mouse	983:1056	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	6	11	theme	endothelial	946:956	arg1	cells					958:962	inflamed endothelial cells	937:962	inflamed endothelial cells under fluid shear	937:980	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	7	12	theme	inflammation	1104:1115	arg1	marrow					1126:1131	bone marrow	1121:1131	bone marrow	1121:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	12	theme	inflammation	1104:1115	arg1	sites					1089:1093	sites	1089:1093	sites of acute inflammation and bone marrow	1089:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	12	theme	inflammation	1104:1115	arg1	inflammation					1104:1115	acute inflammation	1098:1115	acute inflammation	1098:1115	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	13	theme	bone	1121:1124	arg1	marrow					1126:1131	bone marrow	1121:1131	bone marrow	1121:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	14	theme	marrow	1126:1131	arg1	marrow					1126:1131	bone marrow	1121:1131	bone marrow	1121:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	14	theme	marrow	1126:1131	arg1	sites					1089:1093	sites	1089:1093	sites of acute inflammation and bone marrow	1089:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	7	14	theme	marrow	1126:1131	arg1	inflammation					1104:1115	acute inflammation	1098:1115	acute inflammation	1098:1115	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	5	15	theme	intracellular	734:746	arg1	hexosaminidases					748:762	intracellular hexosaminidases	734:762	intracellular hexosaminidases	734:762	The >10-fold greater inhibition is in part due to the resistance of thioglycosides to hydrolysis by intracellular hexosaminidases.					
30344053	2	16	theme	enzymes	324:330	arg1	activity					304:311	the activity	300:311	the activity of natural enzymes	300:330	These compounds divert cellular biosynthetic pathways by acting as artificial substrates that usurp the activity of natural enzymes.					
30344053	4	17	theme	various	515:521	arg1	thioglycosides					523:536	various thioglycosides	515:536	various thioglycosides	515:536	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	6	18	theme	selectin	907:914	arg1	substrates					916:925	selectin substrates	907:925	selectin substrates including inflamed endothelial cells under fluid shear	907:980	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	6	18	theme	selectin	907:914	arg1	cells					958:962	inflamed endothelial cells	937:962	inflamed endothelial cells under fluid shear	937:980	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	2	19	theme	natural	316:322	arg1	enzymes					324:330	natural enzymes	316:330	natural enzymes	316:330	These compounds divert cellular biosynthetic pathways by acting as artificial substrates that usurp the activity of natural enzymes.					
30344053	9	20	theme	leukocyte	1314:1322	arg1	accumulation					1324:1335	inflammatory leukocyte accumulation	1301:1335	inflammatory leukocyte accumulation	1301:1335	They reduce N-/O-linked glycan biosynthesis and inflammatory leukocyte accumulation.					
30344053	3	21	from	concentrations	424:437	arg1	effective					395:403	effective	395:403	effective	395:403	While O-linked glycosides are common, they are only partially effective even at millimolar concentrations.					
30344053	6	22	theme	β-galactose	791:801	arg1	incorporation					803:815	β-galactose incorporation	791:815	β-galactose incorporation into lactosamine chains	791:839	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	4	23	link	O-linked	576:583	arg1	biosynthesis					592:603	O-linked glycan biosynthesis	576:603	O-linked glycan biosynthesis	576:603	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	5	24	theme	greater	647:653	arg1	inhibition					655:664	The >10-fold greater inhibition	634:664	The >10-fold greater inhibition	634:664	The >10-fold greater inhibition is in part due to the resistance of thioglycosides to hydrolysis by intracellular hexosaminidases.					
30344053	5	25	theme	thioglycosides	702:715	arg1	resistance					688:697	the resistance	684:697	the resistance of thioglycosides to hydrolysis by intracellular hexosaminidases	684:762	The >10-fold greater inhibition is in part due to the resistance of thioglycosides to hydrolysis by intracellular hexosaminidases.					
30344053	0	26	theme	Metabolic	29:37	arg1	Decoys					39:44	Efficient Metabolic Decoys	19:44	Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion	19:111	Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion.					
30344053	4	27	theme	10-100 μM	608:616	arg1	concentrations					618:631	10-100 μM concentrations	608:631	10-100 μM concentrations	608:631	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	7	28	theme	neutrophil	1068:1077	arg1	homing					1079:1084	neutrophil homing	1068:1084	neutrophil homing to sites of acute inflammation and bone marrow	1068:1131	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	1	29	theme	Metabolic	114:122	arg1	analogs					145:151	synthetic analogs	135:151	synthetic analogs of naturally occurring biosynthetic acceptors	135:197	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	1	29	theme	Metabolic	114:122	arg1	decoys					124:129	Metabolic decoys	114:129	Metabolic decoys	114:129	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	7	30	theme	acute	1098:1102	arg1	inflammation					1104:1115	acute inflammation	1098:1115	acute inflammation	1098:1115	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	3	31	theme	millimolar	413:422	arg1	concentrations					424:437	millimolar concentrations	413:437	millimolar concentrations	413:437	While O-linked glycosides are common, they are only partially effective even at millimolar concentrations.					
30344053	7	32	theme	∼80	1136:1138	arg1	%					1139:1139	%	1139:1139	%	1139:1139	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	9	33	theme	glycan	1277:1282	arg1	biosynthesis					1284:1295	N-/O-linked glycan biosynthesis	1265:1295	N-/O-linked glycan biosynthesis	1265:1295	They reduce N-/O-linked glycan biosynthesis and inflammatory leukocyte accumulation.					
30344053	3	34	link	O-linked	339:346	arg1	glycosides					348:357	O-linked glycosides	339:357	O-linked glycosides	339:357	While O-linked glycosides are common, they are only partially effective even at millimolar concentrations.					
30344053	8	35	theme	efficient	1211:1219	arg1	inhibitors					1231:1240	efficient metabolic inhibitors	1211:1240	efficient metabolic inhibitors	1211:1240	Overall, thioglycosides represent an easy to synthesize class of efficient metabolic inhibitors or decoys.					
30344053	9	36	theme	N-/O-linked	1265:1275	arg1	biosynthesis					1284:1295	N-/O-linked glycan biosynthesis	1265:1295	N-/O-linked glycan biosynthesis	1265:1295	They reduce N-/O-linked glycan biosynthesis and inflammatory leukocyte accumulation.					
30344053	4	37	theme	glycan	585:590	arg1	biosynthesis					592:603	O-linked glycan biosynthesis	576:603	O-linked glycan biosynthesis	576:603	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	2	38	theme	artificial	267:276	arg1	substrates					278:287	artificial substrates	267:287	artificial substrates that usurp the activity of natural enzymes	267:330	These compounds divert cellular biosynthetic pathways by acting as artificial substrates that usurp the activity of natural enzymes.					
30344053	7	39	theme	-90	1140:1142	arg1	%					1139:1139	%	1139:1139	%	1139:1139	Treatment of granulocytes with thioglycosides prior to infusion into mouse inhibited neutrophil homing to sites of acute inflammation and bone marrow by ∼80%-90%.					
30344053	4	40	theme	O-linked	576:583	arg1	biosynthesis					592:603	O-linked glycan biosynthesis	576:603	O-linked glycan biosynthesis	576:603	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	8	41	theme	inhibitors	1231:1240	arg1	class					1202:1206	class	1202:1206	class of efficient metabolic inhibitors or decoys	1202:1250	Overall, thioglycosides represent an easy to synthesize class of efficient metabolic inhibitors or decoys.					
30344053	6	42	theme	Lewis-X	862:868	arg1	expression					870:879	cell surface sialyl Lewis-X expression	842:879	cell surface sialyl Lewis-X expression	842:879	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	0	43	theme	Glycosylation	49:61	arg1	Decoys					39:44	Efficient Metabolic Decoys	19:44	Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion	19:111	Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion.					
30344053	4	44	dep	surface	561:567	arg1	biosynthesis					592:603	O-linked glycan biosynthesis	576:603	O-linked glycan biosynthesis	576:603	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	4	44	dep	surface	561:567	arg1	N-					569:570	N-	569:570	N-	569:570	In contrast, we report that N-acetylglucosamine (GlcNAc) incorporated into various thioglycosides robustly truncate cell surface N- and O-linked glycan biosynthesis at 10-100 μM concentrations.					
30344053	8	45	theme	metabolic	1221:1229	arg1	inhibitors					1231:1240	efficient metabolic inhibitors	1211:1240	efficient metabolic inhibitors	1211:1240	Overall, thioglycosides represent an easy to synthesize class of efficient metabolic inhibitors or decoys.					
30344053	6	46	theme	fluid	970:974	arg1	shear					976:980	fluid shear	970:980	fluid shear	970:980	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	6	47	theme	sialyl	855:860	arg1	expression					870:879	cell surface sialyl Lewis-X expression	842:879	cell surface sialyl Lewis-X expression	842:879	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	1	48	theme	synthetic	135:143	arg1	analogs					145:151	synthetic analogs	135:151	synthetic analogs of naturally occurring biosynthetic acceptors	135:197	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	1	48	theme	synthetic	135:143	arg1	decoys					124:129	Metabolic decoys	114:129	Metabolic decoys	114:129	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	8	49	theme	decoys	1245:1250	arg1	class					1202:1206	class	1202:1206	class of efficient metabolic inhibitors or decoys	1202:1250	Overall, thioglycosides represent an easy to synthesize class of efficient metabolic inhibitors or decoys.					
30344053	6	50	theme	surface	847:853	arg1	expression					870:879	cell surface sialyl Lewis-X expression	842:879	cell surface sialyl Lewis-X expression	842:879	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	3	51	theme	O-linked	339:346	arg1	glycosides					348:357	O-linked glycosides	339:357	O-linked glycosides	339:357	While O-linked glycosides are common, they are only partially effective even at millimolar concentrations.					
30344053	2	52	theme	biosynthetic	232:243	arg1	pathways					245:252	cellular biosynthetic pathways	223:252	cellular biosynthetic pathways	223:252	These compounds divert cellular biosynthetic pathways by acting as artificial substrates that usurp the activity of natural enzymes.					
30344053	6	53	theme	cell	842:845	arg1	expression					870:879	cell surface sialyl Lewis-X expression	842:879	cell surface sialyl Lewis-X expression	842:879	The thioglycosides reduce β-galactose incorporation into lactosamine chains, cell surface sialyl Lewis-X expression, and leukocyte rolling on selectin substrates including inflamed endothelial cells under fluid shear.					
30344053	2	54	theme	cellular	223:230	arg1	pathways					245:252	cellular biosynthetic pathways	223:252	cellular biosynthetic pathways	223:252	These compounds divert cellular biosynthetic pathways by acting as artificial substrates that usurp the activity of natural enzymes.					
30344053	0	55	theme	Efficient	19:27	arg1	Decoys					39:44	Efficient Metabolic Decoys	19:44	Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion	19:111	Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte Adhesion.					
30344053	1	56	theme	occurring	166:174	arg1	acceptors					189:197	naturally occurring biosynthetic acceptors	156:197	naturally occurring biosynthetic acceptors	156:197	Metabolic decoys are synthetic analogs of naturally occurring biosynthetic acceptors.					
30344053	9	57	link	N-/O-linked	1265:1275	arg1	biosynthesis					1284:1295	N-/O-linked glycan biosynthesis	1265:1295	N-/O-linked glycan biosynthesis	1265:1295	They reduce N-/O-linked glycan biosynthesis and inflammatory leukocyte accumulation.					
30943309	2	0	theme	glycosyltransferases	388:407	arg1	levels					353:358	similar gene expression levels	329:358	similar gene expression levels	329:358	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	2	0	theme	glycosyltransferases	388:407	arg1	activities					374:383	enzymatic activities	364:383	enzymatic activities	364:383	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	4	1	theme	EVs	584:586	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	1	theme	EVs	584:586	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	7	2	link	melanoma-derived	835:850	arg1	EVs					852:854	melanoma-derived EVs	835:854	melanoma-derived EVs	835:854	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	7	3	theme	high	861:864	arg1	expression					866:875	high expression	861:875	high expression of tumor-associated N-glycans	861:905	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	1	4	link	tumor-derived	210:222	arg1	EVs					248:250	EVs	248:250	EVs	248:250	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	4	link	tumor-derived	210:222	arg1	vesicles					238:245	tumor-derived extracellular vesicles	210:245	tumor-derived extracellular vesicles (EVs)	210:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	7	5	theme	tumor	1011:1015	arg1	cells					1017:1021	tumor cells	1011:1021	tumor cells to EVs	1011:1028	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	7	6	theme	melanoma-derived	835:850	arg1	EVs					852:854	melanoma-derived EVs	835:854	melanoma-derived EVs	835:854	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	6	7	from	N-glycomes	800:809	arg1	EVs					814:816	EVs	814:816	EVs	814:816	Sialylation of these N-glycans may generate cell-type-specific N-glycomes on EVs.					
30943309	4	8	theme	de-sialylated	537:549	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	8	theme	de-sialylated	537:549	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	2	9	theme	metastatic	302:311	arg1	potentials					313:322	distinct metastatic potentials	293:322	distinct metastatic potentials	293:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	3	10	contain	have	468:471	arg1	EVs					464:466	EVs	464:466	EVs	464:466	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	3	10	contain	have	468:471	arg1	variants					451:458	All melanoma variants	438:458	All melanoma variants	438:458	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	3	10	contain	have	468:471	arg2	profiles					490:497	nearly identical profiles	473:497	nearly identical profiles of de-sialylated N-glycans	473:524	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	1	11	theme	asparagine-linked	157:173	arg1	N-glycosylation					190:204	N-glycosylation	190:204	N-glycosylation	190:204	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	11	theme	asparagine-linked	157:173	arg1	glycosylation					175:187	asparagine-linked glycosylation	157:187	asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs)	157:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	4	12	dep	core-fucosylated	592:607	arg1	tetra-antennary					610:624	tetra-antennary	610:624	tetra-antennary	610:624	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	7	13	theme	B16	988:990	arg1	melanomas					992:1000	B16 melanomas	988:1000	B16 melanomas	988:1000	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	0	14	from	vesicles	50:57	arg1	melanomas					66:74	B16 melanomas	62:74	B16 melanomas	62:74	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.					
30943309	5	15	theme	N-acetyllactosamine	708:726	arg1	repeats					728:734	N-acetyllactosamine repeats	708:734	N-acetyllactosamine repeats	708:734	A few N-glycans are extended by N-acetyllactosamine repeats.					
30943309	2	16	theme	distinct	293:300	arg1	potentials					313:322	distinct metastatic potentials	293:322	distinct metastatic potentials	293:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	3	17	theme	melanoma	442:449	arg1	variants					451:458	All melanoma variants	438:458	All melanoma variants	438:458	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	4	18	theme	N-glycan	551:558	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	18	theme	N-glycan	551:558	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	2	19	theme	expression	342:351	arg1	levels					353:358	similar gene expression levels	329:358	similar gene expression levels	329:358	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	4	20	theme	major	531:535	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	20	theme	major	531:535	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	0	21	theme	N-glycome	0:8	arg1	inheritance					10:20	N-glycome inheritance	0:20	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas	0:74	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.					
30943309	5	22	theme	few	678:680	arg1	N-glycans					682:690	A few N-glycans	676:690	A few N-glycans	676:690	A few N-glycans are extended by N-acetyllactosamine repeats.					
30943309	2	23	theme	gene	337:340	arg1	levels					353:358	similar gene expression levels	329:358	similar gene expression levels	329:358	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	7	24	theme	selection	968:976	arg1	cycles					978:983	multiple selection cycles	959:983	multiple selection cycles of B16 melanomas	959:1000	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	3	25	theme	identical	480:488	arg1	profiles					490:497	nearly identical profiles	473:497	nearly identical profiles of de-sialylated N-glycans	473:524	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	1	26	link	asparagine-linked	157:173	arg1	N-glycosylation					190:204	N-glycosylation	190:204	N-glycosylation	190:204	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	26	link	asparagine-linked	157:173	arg1	glycosylation					175:187	asparagine-linked glycosylation	157:187	asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs)	157:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	2	27	theme	similar	329:335	arg1	levels					353:358	similar gene expression levels	329:358	similar gene expression levels	329:358	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	4	28	gly	de-sialylated	537:549	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	28	gly	de-sialylated	537:549	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	1	29	theme	tumor-derived	210:222	arg1	EVs					248:250	EVs	248:250	EVs	248:250	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	29	theme	tumor-derived	210:222	arg1	vesicles					238:245	tumor-derived extracellular vesicles	210:245	tumor-derived extracellular vesicles (EVs)	210:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	7	30	theme	structure	921:929	arg1	profile					931:937	the core structure profile	912:937	the core structure profile	912:937	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	7	31	theme	multiple	959:966	arg1	cycles					978:983	multiple selection cycles	959:983	multiple selection cycles of B16 melanomas	959:1000	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	1	32	theme	extracellular	224:236	arg1	EVs					248:250	EVs	248:250	EVs	248:250	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	32	theme	extracellular	224:236	arg1	vesicles					238:245	tumor-derived extracellular vesicles	210:245	tumor-derived extracellular vesicles (EVs)	210:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	0	33	theme	extracellular	36:48	arg1	vesicles					50:57	extracellular vesicles	36:57	extracellular vesicles in B16 melanomas	36:74	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.					
30943309	4	34	theme	β1,6-N-acetylglucosamine	641:664	arg1	branches					666:673	β1,6-N-acetylglucosamine branches	641:673	β1,6-N-acetylglucosamine branches	641:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	3	35	theme	de-sialylated	502:514	arg1	N-glycans					516:524	de-sialylated N-glycans	502:524	de-sialylated N-glycans	502:524	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	6	36	theme	cell-type-specific	781:798	arg1	N-glycomes					800:809	cell-type-specific N-glycomes	781:809	cell-type-specific N-glycomes on EVs	781:816	Sialylation of these N-glycans may generate cell-type-specific N-glycomes on EVs.					
30943309	1	37	theme	vesicles	238:245	arg1	N-glycosylation					190:204	N-glycosylation	190:204	N-glycosylation	190:204	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	37	theme	vesicles	238:245	arg1	behaviors					128:136	metastatic behaviors	117:136	metastatic behaviors of tumor cells	117:151	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	37	theme	vesicles	238:245	arg1	glycosylation					175:187	asparagine-linked glycosylation	157:187	asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs)	157:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	4	38	theme	core-fucosylated	592:607	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	38	theme	core-fucosylated	592:607	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	7	39	theme	melanomas	992:1000	arg1	cycles					978:983	multiple selection cycles	959:983	multiple selection cycles of B16 melanomas	959:1000	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	3	40	theme	N-glycans	516:524	arg1	profiles					490:497	nearly identical profiles	473:497	nearly identical profiles of de-sialylated N-glycans	473:524	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	7	41	theme	core	916:919	arg1	profile					931:937	the core structure profile	912:937	the core structure profile	912:937	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	1	42	theme	metastatic	117:126	arg1	behaviors					128:136	metastatic behaviors	117:136	metastatic behaviors of tumor cells	117:151	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	2	43	with	variants	279:286	arg1	potentials					313:322	distinct metastatic potentials	293:322	distinct metastatic potentials	293:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	4	44	theme	cells	574:578	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	44	theme	cells	574:578	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	3	45	gly	de-sialylated	502:514	arg1	N-glycans					516:524	de-sialylated N-glycans	502:524	de-sialylated N-glycans	502:524	All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans.					
30943309	2	46	theme	enzymatic	364:372	arg1	activities					374:383	enzymatic activities	364:383	enzymatic activities	364:383	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	1	47	gly	glycosylation	175:187	arg1	EVs					248:250	EVs	248:250	EVs	248:250	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	47	gly	glycosylation	175:187	arg1	vesicles					238:245	tumor-derived extracellular vesicles	210:245	tumor-derived extracellular vesicles (EVs)	210:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	47	gly	glycosylation	175:187	arg1	cells					147:151	tumor cells	141:151	tumor cells	141:151	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	7	48	theme	tumor-associated	880:895	arg1	N-glycans					897:905	tumor-associated N-glycans	880:905	tumor-associated N-glycans	880:905	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	4	49	with	N-glycans	626:634	arg1	branches					666:673	β1,6-N-acetylglucosamine branches	641:673	β1,6-N-acetylglucosamine branches	641:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	2	50	theme	B16	275:277	arg1	variants					279:286	Three mouse melanoma B16 variants	254:286	Three mouse melanoma B16 variants with distinct metastatic potentials	254:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	1	51	theme	tumor	141:145	arg1	cells					147:151	tumor cells	141:151	tumor cells	141:151	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	6	52	theme	N-glycans	758:766	arg1	Sialylation					737:747	Sialylation	737:747	Sialylation of these N-glycans	737:766	Sialylation of these N-glycans may generate cell-type-specific N-glycomes on EVs.					
30943309	7	53	theme	N-glycans	897:905	arg1	expression					866:875	high expression	861:875	high expression of tumor-associated N-glycans	861:905	Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.					
30943309	2	54	theme	melanoma	266:273	arg1	variants					279:286	Three mouse melanoma B16 variants	254:286	Three mouse melanoma B16 variants with distinct metastatic potentials	254:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
30943309	4	55	gly	core-fucosylated	592:607	arg1	structures					560:569	The major de-sialylated N-glycan structures	527:569	The major de-sialylated N-glycan structures of cells and EVs	527:586	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	4	55	gly	core-fucosylated	592:607	arg1	N-glycans					626:634	core-fucosylated, tetra-antennary N-glycans	592:634	core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches	592:673	The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches.					
30943309	1	56	theme	cells	147:151	arg1	N-glycosylation					190:204	N-glycosylation	190:204	N-glycosylation	190:204	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	56	theme	cells	147:151	arg1	behaviors					128:136	metastatic behaviors	117:136	metastatic behaviors of tumor cells	117:151	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	1	56	theme	cells	147:151	arg1	glycosylation					175:187	asparagine-linked glycosylation	157:187	asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs)	157:251	We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs).					
30943309	0	57	theme	B16	62:64	arg1	melanomas					66:74	B16 melanomas	62:74	B16 melanomas	62:74	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.					
30943309	0	58	from	cells	27:31	arg1	inheritance					10:20	N-glycome inheritance	0:20	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas	0:74	N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.					
30943309	2	59	theme	mouse	260:264	arg1	variants					279:286	Three mouse melanoma B16 variants	254:286	Three mouse melanoma B16 variants with distinct metastatic potentials	254:322	Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation.					
29909447	4	0	theme	prevalent	530:538	arg1	steps					612:616	laborious sample preparation steps	583:616	laborious sample preparation steps	583:616	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	4	0	theme	prevalent	530:538	arg1	stages					551:556	The most prevalent bottleneck stages	521:556	The most prevalent bottleneck stages for glycomic analyses	521:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	8	1	theme	comparable	1089:1098	arg1	results					1100:1106	very comparable results	1084:1106	very comparable results	1084:1106	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	6	2	theme	high-throughput	766:780	arg1	application					738:748	the successful application	723:748	the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates,	723:847	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	11	3	theme	step	1461:1464	arg1	forward					1466:1472	a step forward	1459:1472	a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1459:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	11	3	theme	step	1461:1464	arg1	procedures					1444:1453	these automated procedures	1428:1453	these automated procedures	1428:1453	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	7	4	theme	starting	1001:1008	arg1	materials					1010:1018	more complex biological starting materials	977:1018	more complex biological starting materials	977:1018	Therefore, we wanted to test if these developed HT methodologies could be applied to more complex biological starting materials.					
29909447	11	5	theme	materials	1599:1607	arg1	analysis					1568:1575	analysis	1568:1575	analysis of complex biological materials	1568:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	10	6	theme	automated	1319:1327	arg1	permethylation					1329:1342	automated permethylation	1319:1342	automated permethylation	1319:1342	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29909447	11	7	theme	profiling	1547:1555	arg1	system					1557:1562	a fully automated, fast and reliable glycomic profiling system	1501:1562	a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1501:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	8	8	theme	based	1049:1053	arg1	method					1070:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	8	9	theme	96-well-plate	1035:1047	arg1	method					1070:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	7	10	theme	complex	982:988	arg1	materials					1010:1018	more complex biological starting materials	977:1018	more complex biological starting materials	977:1018	Therefore, we wanted to test if these developed HT methodologies could be applied to more complex biological starting materials.					
29909447	2	11	theme	stability	302:310	arg1	folding					271:277	protein folding	263:277	protein folding	263:277	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	11	theme	stability	302:310	arg1	half-life					334:342	serum half-life	328:342	serum half-life	328:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	11	theme	stability	302:310	arg1	solubility					313:322	solubility	313:322	solubility	313:322	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	11	theme	stability	302:310	arg1	regulation					280:289	regulation	280:289	regulation of protein stability	280:310	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	6	12	gly	glycoproteins	877:889	arg1	glycoproteins					877:889	purified glycoproteins	868:889	purified glycoproteins	868:889	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	3	13	theme	ways	356:359	arg1	One					345:347	One	345:347	One	345:347	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	3	13	theme	ways	356:359	arg1	ways					356:359	the ways	352:359	the ways	352:359	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	6	14	theme	automated	756:764	arg1	HT					783:784	HT	783:784	HT	783:784	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	6	14	theme	automated	756:764	arg1	high-throughput					766:780	an automated high-throughput	753:780	an automated high-throughput (HT)	753:785	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	6	14	theme	automated	756:764	arg1	protocol					810:817	glycan permethylation protocol	788:817	glycan permethylation protocol based on 96-well microplates	788:846	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	8	15	theme	glycomic	1125:1132	arg1	methodology					1134:1144	established glycomic methodology	1113:1144	established glycomic methodology	1113:1144	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	8	16	theme	automated	1025:1033	arg1	method					1070:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	2	17	theme	protein	294:300	arg1	stability					302:310	protein stability	294:310	protein stability	294:310	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	9	18	theme	similar	1152:1158	arg1	profiles					1169:1176	Very similar glycomic profiles	1147:1176	Very similar glycomic profiles	1147:1176	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	1	19	theme	proteins	187:194	arg1	one					102:104	one	102:104	one	102:104	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	1	19	theme	proteins	187:194	arg1	proteins					187:194	proteins	187:194	proteins	187:194	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	1	19	theme	proteins	187:194	arg1	complex					118:124	the most complex	109:124	the most complex	109:124	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	0	20	theme	complex	44:50	arg1	materials					63:71	complex biological materials	44:71	complex biological materials	44:71	Towards automation of glycomic profiling of complex biological materials.					
29909447	8	21	theme	established	1113:1123	arg1	methodology					1134:1144	established glycomic methodology	1113:1144	established glycomic methodology	1113:1144	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	8	22	theme	permethylation	1055:1068	arg1	method					1070:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method	1021:1075	Our automated 96-well-plate based permethylation method showed very comparable results with established glycomic methodology.					
29909447	9	23	theme	glycomic	1160:1167	arg1	profiles					1169:1176	Very similar glycomic profiles	1147:1176	Very similar glycomic profiles	1147:1176	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	11	24	theme	system	1557:1562	arg1	development					1486:1496	the development	1482:1496	the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1482:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	3	25	theme	structural	398:407	arg1	characterizations					409:425	systematic structural characterizations	387:425	systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS)	387:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	5	26	theme	sample	663:668	arg1	preparations					670:681	sample preparations	663:681	sample preparations	663:681	Therefore, in this study, we aim to improve sample preparations by automation.					
29909447	6	27	theme	96-well	828:834	arg1	microplates					836:846	96-well microplates	828:846	96-well microplates	828:846	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	11	28	theme	glycomic	1538:1545	arg1	system					1557:1562	a fully automated, fast and reliable glycomic profiling system	1501:1562	a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1501:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	3	29	theme	glycomics	462:470	arg1	methodology					472:482	glycomics methodology	462:482	glycomics methodology based around mass spectrometry (MS)	462:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	4	30	theme	preparation	600:610	arg1	stages					551:556	The most prevalent bottleneck stages	521:556	The most prevalent bottleneck stages for glycomic analyses	521:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	4	30	theme	preparation	600:610	arg1	steps					612:616	laborious sample preparation steps	583:616	laborious sample preparation steps	583:616	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	2	31	theme	serum	328:332	arg1	half-life					334:342	serum half-life	328:342	serum half-life	328:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	6	32	theme	successful	727:736	arg1	application					738:748	the successful application	723:748	the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates,	723:847	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	0	33	theme	glycomic	22:29	arg1	profiling					31:39	glycomic profiling	22:39	glycomic profiling of complex biological materials	22:71	Towards automation of glycomic profiling of complex biological materials.					
29909447	4	34	theme	sample	593:598	arg1	stages					551:556	The most prevalent bottleneck stages	521:556	The most prevalent bottleneck stages for glycomic analyses	521:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	4	34	theme	sample	593:598	arg1	steps					612:616	laborious sample preparation steps	583:616	laborious sample preparation steps	583:616	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	11	35	theme	complex	1580:1586	arg1	materials					1599:1607	complex biological materials	1580:1607	complex biological materials	1580:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	3	36	theme	systematic	387:396	arg1	characterizations					409:425	systematic structural characterizations	387:425	systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS)	387:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	3	37	theme	glycosylation	438:450	arg1	characterizations					409:425	systematic structural characterizations	387:425	systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS)	387:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	9	38	attach	derived	1234:1240	arg1	tissues					1267:1273	heterogeneous mouse tissues	1247:1273	heterogeneous mouse tissues	1247:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	9	38	attach	derived	1234:1240	arg2	mixtures					1225:1232	complex glycoprotein/protein mixtures	1196:1232	complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues	1196:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	10	39	theme	samples	1347:1353	arg1	release					1295:1301	Automated N-glycan release	1276:1301	Automated N-glycan release	1276:1301	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29909447	10	39	theme	samples	1347:1353	arg1	enrichment					1304:1313	enrichment	1304:1313	enrichment	1304:1313	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29909447	10	39	theme	samples	1347:1353	arg1	permethylation					1329:1342	automated permethylation	1319:1342	automated permethylation	1319:1342	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29909447	9	40	theme	complex	1196:1202	arg1	mixtures					1225:1232	complex glycoprotein/protein mixtures	1196:1232	complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues	1196:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	3	41	theme	mass	497:500	arg1	spectrometry					502:513	mass spectrometry	497:513	mass spectrometry (MS)	497:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	3	41	theme	mass	497:500	arg1	MS					516:517	MS	516:517	MS	516:517	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	0	42	theme	profiling	31:39	arg1	automation					8:17	automation	8:17	automation of glycomic profiling of complex biological materials	8:71	Towards automation of glycomic profiling of complex biological materials.					
29909447	11	43	theme	automated	1434:1442	arg1	forward					1466:1472	a step forward	1459:1472	a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1459:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	11	43	theme	automated	1434:1442	arg1	procedures					1444:1453	these automated procedures	1428:1453	these automated procedures	1428:1453	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	9	44	theme	glycoprotein/protein	1204:1223	arg1	mixtures					1225:1232	complex glycoprotein/protein mixtures	1196:1232	complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues	1196:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	0	45	theme	biological	52:61	arg1	materials					63:71	complex biological materials	44:71	complex biological materials	44:71	Towards automation of glycomic profiling of complex biological materials.					
29909447	4	46	theme	glycomic	562:569	arg1	analyses					571:578	glycomic analyses	562:578	glycomic analyses	562:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	11	47	dep	automated	1509:1517	arg1	reliable					1529:1536	reliable	1529:1536	reliable	1529:1536	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	11	47	dep	automated	1509:1517	arg1	fast					1520:1523	fast	1520:1523	fast	1520:1523	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	6	48	theme	permethylation	795:808	arg1	high-throughput					766:780	an automated high-throughput	753:780	an automated high-throughput (HT)	753:785	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	6	48	theme	permethylation	795:808	arg1	protocol					810:817	glycan permethylation protocol	788:817	glycan permethylation protocol based on 96-well microplates	788:846	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	1	49	theme	most	113:116	arg1	complex					118:124	the most complex	109:124	the most complex	109:124	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	3	50	theme	protein	430:436	arg1	glycosylation					438:450	protein glycosylation	430:450	protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS)	430:518	One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS).					
29909447	4	51	theme	laborious	583:591	arg1	stages					551:556	The most prevalent bottleneck stages	521:556	The most prevalent bottleneck stages for glycomic analyses	521:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	4	51	theme	laborious	583:591	arg1	steps					612:616	laborious sample preparation steps	583:616	laborious sample preparation steps	583:616	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	2	52	theme	many	229:232	arg1	folding					271:277	protein folding	263:277	protein folding	263:277	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	52	theme	many	229:232	arg1	regulation					280:289	regulation	280:289	regulation of protein stability	280:310	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	52	theme	many	229:232	arg1	processes					245:253	many biological processes	229:253	many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life	229:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	52	theme	many	229:232	arg1	solubility					313:322	solubility	313:322	solubility	313:322	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	52	theme	many	229:232	arg1	half-life					334:342	serum half-life	328:342	serum half-life	328:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	53	theme	biological	234:243	arg1	folding					271:277	protein folding	263:277	protein folding	263:277	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	53	theme	biological	234:243	arg1	regulation					280:289	regulation	280:289	regulation of protein stability	280:310	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	53	theme	biological	234:243	arg1	processes					245:253	many biological processes	229:253	many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life	229:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	53	theme	biological	234:243	arg1	solubility					313:322	solubility	313:322	solubility	313:322	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	2	53	theme	biological	234:243	arg1	half-life					334:342	serum half-life	328:342	serum half-life	328:342	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	6	54	theme	glycan	788:793	arg1	high-throughput					766:780	an automated high-throughput	753:780	an automated high-throughput (HT)	753:785	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	6	54	theme	glycan	788:793	arg1	protocol					810:817	glycan permethylation protocol	788:817	glycan permethylation protocol based on 96-well microplates	788:846	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	1	55	theme	complex	118:124	arg1	one					102:104	one	102:104	one	102:104	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	1	55	theme	complex	118:124	arg1	proteins					187:194	proteins	187:194	proteins	187:194	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	1	55	theme	complex	118:124	arg1	complex					118:124	the most complex	109:124	the most complex	109:124	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	7	56	dep	complex	982:988	arg1	biological					990:999	biological	990:999	biological	990:999	Therefore, we wanted to test if these developed HT methodologies could be applied to more complex biological starting materials.					
29909447	6	57	theme	glycoproteins	877:889	arg1	analysis					856:863	the analysis	852:863	the analysis of purified glycoproteins	852:889	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	6	58	theme	purified	868:875	arg1	glycoproteins					877:889	purified glycoproteins	868:889	purified glycoproteins	868:889	We recently demonstrated the successful application of an automated high-throughput (HT), glycan permethylation protocol based on 96-well microplates, in the analysis of purified glycoproteins.					
29909447	9	59	theme	heterogeneous	1247:1259	arg1	tissues					1267:1273	heterogeneous mouse tissues	1247:1273	heterogeneous mouse tissues	1247:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	5	60	from	aim	648:650	arg1	study					638:642	this study	633:642	this study	633:642	Therefore, in this study, we aim to improve sample preparations by automation.					
29909447	7	61	theme	developed	930:938	arg1	methodologies					943:955	these developed HT methodologies	924:955	these developed HT methodologies	924:955	Therefore, we wanted to test if these developed HT methodologies could be applied to more complex biological starting materials.					
29909447	11	62	theme	biological	1588:1597	arg1	materials					1599:1607	complex biological materials	1580:1607	complex biological materials	1580:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	2	63	theme	important	210:218	arg1	roles					220:224	important roles	210:224	important roles	210:224	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	9	64	theme	mouse	1261:1265	arg1	tissues					1267:1273	heterogeneous mouse tissues	1247:1273	heterogeneous mouse tissues	1247:1273	Very similar glycomic profiles were obtained for complex glycoprotein/protein mixtures derived from heterogeneous mouse tissues.					
29909447	1	65	theme	diverse	143:149	arg1	modifications					170:182	structurally diverse post-translational modifications	130:182	structurally diverse post-translational modifications	130:182	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	7	66	theme	HT	940:941	arg1	methodologies					943:955	these developed HT methodologies	924:955	these developed HT methodologies	924:955	Therefore, we wanted to test if these developed HT methodologies could be applied to more complex biological starting materials.					
29909447	10	67	theme	N-glycan	1286:1293	arg1	release					1295:1301	Automated N-glycan release	1276:1301	Automated N-glycan release	1276:1301	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29909447	0	68	theme	materials	63:71	arg1	profiling					31:39	glycomic profiling	22:39	glycomic profiling of complex biological materials	22:71	Towards automation of glycomic profiling of complex biological materials.					
29909447	4	69	theme	bottleneck	540:549	arg1	steps					612:616	laborious sample preparation steps	583:616	laborious sample preparation steps	583:616	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	4	69	theme	bottleneck	540:549	arg1	stages					551:556	The most prevalent bottleneck stages	521:556	The most prevalent bottleneck stages for glycomic analyses	521:578	The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps.					
29909447	2	70	theme	protein	263:269	arg1	folding					271:277	protein folding	263:277	protein folding	263:277	Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life.					
29909447	1	71	theme	post-translational	151:168	arg1	modifications					170:182	structurally diverse post-translational modifications	130:182	structurally diverse post-translational modifications	130:182	Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins.					
29909447	11	72	theme	automated	1509:1517	arg1	system					1557:1562	a fully automated, fast and reliable glycomic profiling system	1501:1562	a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials	1501:1607	Therefore we conclude that these automated procedures are a step forward towards the development of a fully automated, fast and reliable glycomic profiling system for analysis of complex biological materials.					
29909447	10	73	theme	Automated	1276:1284	arg1	release					1295:1301	Automated N-glycan release	1276:1301	Automated N-glycan release	1276:1301	Automated N-glycan release, enrichment and automated permethylation of samples proved to be convenient, robust and reliable.					
29579062	3	0	theme	differing	678:686	arg1	sensitivity					688:698	differing sensitivity	678:698	differing sensitivity to anti-HIV Env antibodies	678:725	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	11	1	theme	HIV-1	1974:1978	arg1	isolates					1980:1987	HIV-1 isolates	1974:1987	HIV-1 isolates	1974:1987	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	4	2	theme	α1-2Man	840:846	arg1	lectins					856:862	α1-2Man binding lectins	840:862	α1-2Man binding lectins	840:862	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	9	3	from	enrichment	1625:1634	arg1	virus					1667:1671	the virus	1663:1671	the virus	1663:1671	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	6	4	theme	homogenous	1200:1209	arg1	glycans					1211:1217	more homogenous glycans	1195:1217	more homogenous glycans	1195:1217	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	3	5	theme	Env	712:714	arg1	antibodies					716:725	anti-HIV Env antibodies	703:725	anti-HIV Env antibodies	703:725	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	10	6	theme	HIV-1	1852:1856	arg1	glycans					1862:1868	HIV-1 Env glycans	1852:1868	HIV-1 Env glycans	1852:1868	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	1	7	theme	envelope	164:171	arg1	glycoprotein					179:190	HIV-1 envelope (Env) glycoprotein	158:190	HIV-1 envelope (Env) glycoprotein	158:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	10	8	from	resistant	1825:1833	arg1	addition					1677:1684	addition	1677:1684	addition	1677:1684	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	1	9	theme	Env	174:176	arg1	glycoprotein					179:190	HIV-1 envelope (Env) glycoprotein	158:190	HIV-1 envelope (Env) glycoprotein	158:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	6	10	theme	inhibitors	1173:1182	arg1	presence					1139:1146	the presence	1135:1146	the presence of different glycosidase inhibitors to express more homogenous glycans	1135:1217	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	2	11	theme	Env	323:325	arg1	interaction					334:344	Env glycan interaction	323:344	Env glycan interaction	323:344	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	1	12	theme	glycoprotein	179:190	arg1	surface					147:153	the surface	143:153	the surface of HIV-1 envelope (Env) glycoprotein	143:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	8	13	theme	α1-3Man-	1342:1349	arg1	lectins					1371:1377	The α1-3Man- and α1-6Man-binding lectins	1338:1377	The α1-3Man- and α1-6Man-binding lectins	1338:1377	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	8	13	theme	α1-3Man-	1342:1349	arg1	potent					1394:1399	potent	1394:1399	potent	1394:1399	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	10	14	theme	mannose-type	1722:1733	arg1	glycans					1735:1741	mannose-type glycans	1722:1741	mannose-type glycans	1722:1741	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	8	15	theme	α1-6Man-binding	1355:1369	arg1	lectins					1371:1377	The α1-3Man- and α1-6Man-binding lectins	1338:1377	The α1-3Man- and α1-6Man-binding lectins	1338:1377	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	8	15	theme	α1-6Man-binding	1355:1369	arg1	potent					1394:1399	potent	1394:1399	potent	1394:1399	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	3	16	theme	distinct	573:580	arg1	oligosaccharides					582:597	distinct oligosaccharides	573:597	distinct oligosaccharides	573:597	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	8	17	with	Man5GlcNAc2	1442:1452	arg1	α1-3Man					1492:1498	terminal α1-3Man	1483:1498	terminal α1-3Man	1483:1498	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	8	17	with	Man5GlcNAc2	1442:1452	arg1	α1-6Man					1504:1510	α1-6Man	1504:1510	α1-6Man	1504:1510	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	5	18	theme	Env	952:954	arg1	components					932:941	the oligosaccharide components	912:941	the oligosaccharide components of virus Env	912:954	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	0	19	theme	HIV-1	54:58	arg1	envelope					60:67	HIV-1 envelope	54:67	HIV-1 envelope	54:67	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	11	20	theme	glycans	2084:2090	arg1	microheterogeneity					2053:2070	the microheterogeneity	2049:2070	the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein	2049:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	2	21	theme	virus-neutralization	462:481	arg1	sensitivity					483:493	virus-neutralization sensitivity	462:493	virus-neutralization sensitivity to lectins	462:504	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	11	22	theme	glycoprotein	2134:2145	arg1	surface					2109:2115	the surface	2105:2115	the surface of the virus Env glycoprotein	2105:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	8	23	with	glycans	1470:1476	arg1	α1-3Man					1492:1498	terminal α1-3Man	1483:1498	terminal α1-3Man	1483:1498	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	8	23	with	glycans	1470:1476	arg1	α1-6Man					1504:1510	α1-6Man	1504:1510	α1-6Man	1504:1510	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	7	24	theme	Man5-9GlcNAc2	1261:1273	arg1	glycans					1275:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	Viruses enriched for α1-2Man terminating Man5-9GlcNAc2 glycans became similarly sensitive to α1-2Man-binding lectins.					
29579062	5	25	theme	oligosaccharide	916:930	arg1	components					932:941	the oligosaccharide components	912:941	the oligosaccharide components of virus Env	912:954	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	9	26	from	glycans	1652:1658	arg1	virus					1667:1671	the virus	1663:1671	the virus	1663:1671	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	0	27	from	Heterogeneity	0:12	arg1	composition					24:34	glycan composition	17:34	glycan composition	17:34	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	0	27	from	Heterogeneity	0:12	arg1	surface					43:49	the surface	39:49	the surface of HIV-1 envelope	39:67	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	7	28	theme	terminating	1249:1259	arg1	glycans					1275:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	Viruses enriched for α1-2Man terminating Man5-9GlcNAc2 glycans became similarly sensitive to α1-2Man-binding lectins.					
29579062	7	29	theme	α1-2Man	1241:1247	arg1	glycans					1275:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	α1-2Man terminating Man5-9GlcNAc2 glycans	1241:1281	Viruses enriched for α1-2Man terminating Man5-9GlcNAc2 glycans became similarly sensitive to α1-2Man-binding lectins.					
29579062	5	30	theme	glycan	981:986	arg1	heterogeneity					988:1000	glycan heterogeneity	981:1000	glycan heterogeneity among HIV-1 isolates	981:1021	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	10	31	theme	Endo-H	1761:1766	arg1	deglycosylation					1768:1782	Endo-H deglycosylation	1761:1782	Endo-H deglycosylation	1761:1782	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	2	32	theme	HIV-1	426:430	arg1	isolates					432:439	HIV-1 isolates	426:439	HIV-1 isolates	426:439	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	8	33	theme	hybrid	1458:1463	arg1	glycans					1470:1476	hybrid type glycans	1458:1476	hybrid type glycans with terminal α1-3Man and α1-6Man	1458:1510	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	11	34	theme	differential	1997:2008	arg1	sensitivity					2010:2020	differential sensitivity	1997:2020	differential sensitivity to lectins	1997:2031	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	4	35	theme	HIV-1	752:756	arg1	isolates					758:765	HIV-1 isolates	752:765	HIV-1 isolates	752:765	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	2	36	theme	Env	395:397	arg1	heterogeneity					406:418	Env glycan heterogeneity	395:418	Env glycan heterogeneity among HIV-1 isolates	395:439	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	0	37	theme	glycan	17:22	arg1	composition					24:34	glycan composition	17:34	glycan composition	17:34	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	3	38	theme	HIV-1	650:654	arg1	viruses					656:662	HIV-1 viruses	650:662	HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies	650:725	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	8	39	theme	terminal	1483:1490	arg1	α1-3Man					1492:1498	terminal α1-3Man	1483:1498	terminal α1-3Man	1483:1498	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	9	40	theme	lectin-mediated	1526:1540	arg1	inhibition					1542:1551	lectin-mediated inhibition	1526:1551	lectin-mediated inhibition	1526:1551	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	5	41	theme	differential	1040:1051	arg1	sensitivity					1053:1063	this differential sensitivity	1035:1063	this differential sensitivity	1035:1063	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	11	42	gly	microheterogeneity	2053:2070	arg1	glycans					2084:2090	N-linked glycans	2075:2090	N-linked glycans expressed on the surface of the virus Env glycoprotein	2075:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	10	43	theme	mannose	1924:1930	arg1	types					1932:1936	mannose types	1924:1936	mannose types	1924:1936	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	1	44	contain	have	192:195	arg2	potential					201:209	the potential	197:209	the potential for use as antiviral agents	197:237	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	1	44	contain	have	192:195	arg1	Lectins					110:116	Lectins	110:116	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein	110:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	10	45	theme	viruses	1802:1808	arg1	Env					1785:1787	Env	1785:1787	Env of untreated viruses	1785:1808	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	11	46	gly	glycoprotein	2134:2145	arg1	glycoprotein					2134:2145	the virus Env glycoprotein	2120:2145	the virus Env glycoprotein	2120:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	7	47	theme	α1-2Man-binding	1313:1327	arg1	lectins					1329:1335	α1-2Man-binding lectins	1313:1335	α1-2Man-binding lectins	1313:1335	Viruses enriched for α1-2Man terminating Man5-9GlcNAc2 glycans became similarly sensitive to α1-2Man-binding lectins.					
29579062	3	48	theme	anti-HIV	703:710	arg1	antibodies					716:725	anti-HIV Env antibodies	703:725	anti-HIV Env antibodies	703:725	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	2	49	theme	glycan	327:332	arg1	interaction					334:344	Env glycan interaction	323:344	Env glycan interaction	323:344	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	1	50	theme	antiviral	222:230	arg1	agents					232:237	antiviral agents	222:237	antiviral agents	222:237	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	2	51	theme	molecular	291:299	arg1	details					301:307	the molecular details	287:307	the molecular details of lectin and Env glycan interaction	287:344	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	1	52	gly	glycoprotein	179:190	arg1	glycoprotein					179:190	HIV-1 envelope (Env) glycoprotein	158:190	HIV-1 envelope (Env) glycoprotein	158:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	10	53	theme	Env	1858:1860	arg1	glycans					1862:1868	HIV-1 Env glycans	1852:1868	HIV-1 Env glycans	1852:1868	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	1	54	theme	HIV-1	158:162	arg1	glycoprotein					179:190	HIV-1 envelope (Env) glycoprotein	158:190	HIV-1 envelope (Env) glycoprotein	158:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	10	55	theme	viruses	1700:1706	arg1	Env					1693:1695	Env	1693:1695	Env of viruses enriched with mannose-type glycans	1693:1741	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	3	56	theme	fine	552:555	arg1	specificity					557:567	fine specificity	552:567	fine specificity for distinct oligosaccharides	552:597	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	4	57	theme	binding	848:854	arg1	lectins					856:862	α1-2Man binding lectins	840:862	α1-2Man binding lectins	840:862	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	3	58	theme	lectins	539:545	arg1	panel					530:534	a panel	528:534	a panel of lectins with fine specificity for distinct oligosaccharides	528:597	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	6	59	theme	glycosidase	1161:1171	arg1	inhibitors					1173:1182	different glycosidase inhibitors	1151:1182	different glycosidase inhibitors	1151:1182	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	9	60	theme	mannose-type	1639:1650	arg1	glycans					1652:1658	mannose-type glycans	1639:1658	mannose-type glycans on the virus	1639:1671	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	4	61	contain	have	767:770	arg2	sensitivity					782:792	different sensitivity	772:792	different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins	772:862	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	4	61	contain	have	767:770	arg1	isolates					758:765	HIV-1 isolates	752:765	HIV-1 isolates	752:765	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	5	62	theme	virus	946:950	arg1	Env					952:954	virus Env	946:954	virus Env	946:954	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	11	63	theme	virus	2124:2128	arg1	glycoprotein					2134:2145	the virus Env glycoprotein	2120:2145	the virus Env glycoprotein	2120:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	0	64	theme	envelope	60:67	arg1	surface					43:49	the surface	39:49	the surface of HIV-1 envelope	39:67	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	11	65	theme	N-linked	2075:2082	arg1	glycans					2084:2090	N-linked glycans	2075:2090	N-linked glycans expressed on the surface of the virus Env glycoprotein	2075:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	2	66	from	heterogeneity	406:418	arg1	sensitivity					483:493	virus-neutralization sensitivity	462:493	virus-neutralization sensitivity to lectins	462:504	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	0	67	theme	virus	80:84	arg1	sensitivity					86:96	virus sensitivity	80:96	virus sensitivity to lectins	80:107	Heterogeneity in glycan composition on the surface of HIV-1 envelope determines virus sensitivity to lectins.					
29579062	9	68	from	virus	1667:1671	arg1	enrichment					1625:1634	enrichment	1625:1634	enrichment of mannose-type glycans on the virus	1625:1671	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	9	69	theme	glycans	1652:1658	arg1	enrichment					1625:1634	enrichment	1625:1634	enrichment of mannose-type glycans on the virus	1625:1671	Furthermore, lectin-mediated inhibition was competitively alleviated by mannan and this effect was augmented by enrichment of mannose-type glycans on the virus.					
29579062	11	70	theme	Env	2130:2132	arg1	glycoprotein					2134:2145	the virus Env glycoprotein	2120:2145	the virus Env glycoprotein	2120:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
29579062	6	71	theme	acute	1104:1108	arg1	viruses					1110:1116	chronic and acute viruses	1092:1116	chronic and acute viruses	1092:1116	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	2	72	from	influence	449:457	arg1	sensitivity					483:493	virus-neutralization sensitivity	462:493	virus-neutralization sensitivity to lectins	462:504	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	4	73	theme	different	772:780	arg1	sensitivity					782:792	different sensitivity	772:792	different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins	772:862	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	3	74	with	lectins	539:545	arg1	specificity					557:567	fine specificity	552:567	fine specificity for distinct oligosaccharides	552:597	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	1	75	from	N-glycans	130:138	arg1	surface					147:153	the surface	143:153	the surface of HIV-1 envelope (Env) glycoprotein	143:190	Lectins that target N-glycans on the surface of HIV-1 envelope (Env) glycoprotein have the potential for use as antiviral agents.					
29579062	6	76	theme	chronic	1092:1098	arg1	viruses					1110:1116	chronic and acute viruses	1092:1116	chronic and acute viruses	1092:1116	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	2	77	theme	further	347:353	arg1	studies					355:361	further studies	347:361	further studies	347:361	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	2	78	theme	glycan	399:404	arg1	heterogeneity					406:418	Env glycan heterogeneity	395:418	Env glycan heterogeneity among HIV-1 isolates	395:439	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	8	79	theme	type	1465:1468	arg1	glycans					1470:1476	hybrid type glycans	1458:1476	hybrid type glycans with terminal α1-3Man and α1-6Man	1458:1510	The α1-3Man- and α1-6Man-binding lectins also were more potent against viruses expressing predominantly Man5GlcNAc2 and hybrid type glycans with terminal α1-3Man and α1-6Man.					
29579062	6	80	theme	different	1151:1159	arg1	inhibitors					1173:1182	different glycosidase inhibitors	1151:1182	different glycosidase inhibitors	1151:1182	To evaluate this further, chronic and acute viruses were produced in the presence of different glycosidase inhibitors to express more homogenous glycans.					
29579062	2	81	theme	interaction	334:344	arg1	details					301:307	the molecular details	287:307	the molecular details of lectin and Env glycan interaction	287:344	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	2	82	theme	lectin	312:317	arg1	details					301:307	the molecular details	287:307	the molecular details of lectin and Env glycan interaction	287:344	Although progress has been made in deciphering the molecular details of lectin and Env glycan interaction, further studies are needed to better understand Env glycan heterogeneity among HIV-1 isolates and its influence on virus-neutralization sensitivity to lectins.					
29579062	5	83	theme	HIV-1	1008:1012	arg1	isolates					1014:1021	HIV-1 isolates	1008:1021	HIV-1 isolates	1008:1021	Considering that lectins exclusively recognize the oligosaccharide components of virus Env, these data suggest that glycan heterogeneity among HIV-1 isolates may explain this differential sensitivity.					
29579062	3	84	theme	viruses	656:662	arg1	infection					637:645	infection	637:645	infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies	637:725	This study evaluated a panel of lectins with fine specificity for distinct oligosaccharides and assessed their ability to inhibit infection of HIV-1 viruses known to have differing sensitivity to anti-HIV Env antibodies.					
29579062	10	85	theme	untreated	1792:1800	arg1	viruses					1802:1808	untreated viruses	1792:1808	untreated viruses	1792:1808	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	10	86	from	addition	1677:1684	arg1	resistant					1825:1833	resistant	1825:1833	resistant	1825:1833	In addition, while Env of viruses enriched with mannose-type glycans were sensitive to Endo-H deglycosylation, Env of untreated viruses were partially resistant, indicating that HIV-1 Env glycans are heterogeneously comprised of complex, hybrid, and mannose types.					
29579062	4	87	theme	specific	805:812	arg1	lectins					797:803	lectins	797:803	lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins	797:862	The results showed that HIV-1 isolates have different sensitivity to lectins specific for α1-3Man, α1-6Man, and α1-2Man binding lectins.					
29579062	11	88	link	N-linked	2075:2082	arg1	glycans					2084:2090	N-linked glycans	2075:2090	N-linked glycans expressed on the surface of the virus Env glycoprotein	2075:2145	Overall, our data demonstrate that HIV-1 isolates display differential sensitivity to lectins, in part due to the microheterogeneity of N-linked glycans expressed on the surface of the virus Env glycoprotein.					
31081628	5	0	theme	lipid-linked	767:778	arg1	intermediates					799:811	intermediates	799:811	intermediates directly used in glycan biosynthesis	799:848	We reasoned that lipid-linked glycans, which are intermediates directly used in glycan biosynthesis, would offer an alternative.					
31081628	5	0	theme	lipid-linked	767:778	arg1	glycans					780:786	lipid-linked glycans	767:786	lipid-linked glycans	767:786	We reasoned that lipid-linked glycans, which are intermediates directly used in glycan biosynthesis, would offer an alternative.					
31081628	6	1	from	strategy	946:953	arg1	glutamicum					974:983	Corynebacterium glutamicum	958:983	Corynebacterium glutamicum	958:983	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	6	1	from	strategy	946:953	arg1	smegmatis					1003:1011	Mycobacterium smegmatis	989:1011	Mycobacterium smegmatis	989:1011	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	6	1	from	strategy	946:953	arg1	organisms					1014:1022	organisms	1014:1022	organisms that serve as models of Mycobacterium tuberculosis	1014:1073	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	9	2	theme	known	1482:1486	arg1	transferases					1505:1516	all five known arabinofuranosyl transferases	1473:1516	all five known arabinofuranosyl transferases	1473:1516	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	8	3	theme	labeled	1367:1373	arg1	substrate					1382:1390	an isotopically labeled glycan substrate	1351:1390	an isotopically labeled glycan substrate	1351:1390	The addition of an isotopically labeled glycan substrate facilitated cell wall characterization by NMR.					
31081628	1	4	from	ubiquity	74:81	arg1	biology					112:118	biology	112:118	biology	112:118	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	2	5	theme	nucleotide-sugar	317:332	arg1	intermediates					334:346	nucleotide-sugar intermediates	317:346	nucleotide-sugar intermediates	317:346	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	10	6	theme	wild-type	1665:1673	arg1	cells					1675:1679	wild-type cells	1665:1679	wild-type cells treated with an inhibitor of cell wall biosynthesis	1665:1731	The lipid-based probe could also rescue wild-type cells treated with an inhibitor of cell wall biosynthesis.					
31081628	11	7	theme	powerful	1898:1905	arg1	strategy					1907:1914	a powerful strategy	1896:1914	a powerful strategy for probing glycan structure and function	1896:1956	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	11	7	theme	powerful	1898:1905	arg1	incorporation					1879:1891	biosynthetic incorporation	1866:1891	biosynthetic incorporation	1866:1891	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	11	8	theme	biosynthetic	1866:1877	arg1	strategy					1907:1914	a powerful strategy	1896:1914	a powerful strategy for probing glycan structure and function	1896:1956	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	11	8	theme	biosynthetic	1866:1877	arg1	incorporation					1879:1891	biosynthetic incorporation	1866:1891	biosynthetic incorporation	1866:1891	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	7	9	theme	glycosyl	1150:1157	arg1	donors					1159:1164	synthetic glycosyl donors	1140:1164	synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation	1140:1332	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	7	10	theme	biosynthetic	1266:1277	arg1	intermediates					1279:1291	biosynthetic intermediates	1266:1291	biosynthetic intermediates	1266:1291	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	7	10	theme	biosynthetic	1266:1277	arg1	glycolipids					1241:1251	non-natural glycolipids	1229:1251	non-natural glycolipids	1229:1251	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	11	11	theme	lipid-linked	1779:1790	arg1	glycans					1792:1798	natural lipid-linked glycans	1771:1798	natural lipid-linked glycans	1771:1798	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	1	12	dep	methods	121:127	arg1	probe					132:136	probe	132:136	to probe their structures in cells	129:162	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	7	13	theme	chemical	1309:1316	arg1	complementation					1318:1332	chemical complementation	1309:1332	chemical complementation	1309:1332	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	2	14	theme	non-natural	262:272	arg1	sugars					274:279	non-natural sugars	262:279	non-natural sugars	262:279	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	9	15	theme	cell	1610:1613	arg1	envelope					1615:1622	the cell envelope	1606:1622	the cell envelope	1606:1622	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	7	16	dep	C.	1084:1085	arg1	glutamicum					1087:1096	glutamicum	1087:1096	glutamicum	1087:1096	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	6	17	theme	arabinofuranosyl	902:917	arg1	phospholipids					919:931	synthetic arabinofuranosyl phospholipids	892:931	synthetic arabinofuranosyl phospholipids	892:931	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	9	18	theme	exogenous	1536:1544	arg1	donor					1565:1569	the exogenous lipid-linked sugar donor	1532:1569	the exogenous lipid-linked sugar donor	1532:1569	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	9	19	theme	lipid-linked	1546:1557	arg1	donor					1565:1569	the exogenous lipid-linked sugar donor	1532:1569	the exogenous lipid-linked sugar donor	1532:1569	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	7	20	theme	cell	1190:1193	arg1	arabinan					1200:1207	cell wall arabinan	1190:1207	cell wall arabinan	1190:1207	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	6	21	theme	synthetic	892:900	arg1	phospholipids					919:931	synthetic arabinofuranosyl phospholipids	892:931	synthetic arabinofuranosyl phospholipids	892:931	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	7	22	theme	synthetic	1140:1148	arg1	donors					1159:1164	synthetic glycosyl donors	1140:1164	synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation	1140:1332	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	7	23	theme	non-natural	1229:1239	arg1	intermediates					1279:1291	biosynthetic intermediates	1266:1291	biosynthetic intermediates	1266:1291	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	7	23	theme	non-natural	1229:1239	arg1	glycolipids					1241:1251	non-natural glycolipids	1229:1251	non-natural glycolipids	1229:1251	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	11	24	link	lipid-linked	1779:1790	arg1	glycans					1792:1798	natural lipid-linked glycans	1771:1798	natural lipid-linked glycans	1771:1798	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	9	25	theme	sugar	1559:1563	arg1	donor					1565:1569	the exogenous lipid-linked sugar donor	1532:1569	the exogenous lipid-linked sugar donor	1532:1569	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	0	26	theme	Cell	10:13	arg1	Wall					15:18	Bacterial Cell Wall	0:18	Bacterial Cell Wall	0:18	Bacterial Cell Wall Modification with a Glycolipid Substrate.					
31081628	11	27	theme	natural	1771:1777	arg1	glycans					1792:1798	natural lipid-linked glycans	1771:1798	natural lipid-linked glycans	1771:1798	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	9	28	link	lipid-linked	1546:1557	arg1	donor					1565:1569	the exogenous lipid-linked sugar donor	1532:1569	the exogenous lipid-linked sugar donor	1532:1569	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	5	29	link	lipid-linked	767:778	arg1	intermediates					799:811	intermediates	799:811	intermediates directly used in glycan biosynthesis	799:848	We reasoned that lipid-linked glycans, which are intermediates directly used in glycan biosynthesis, would offer an alternative.					
31081628	5	29	link	lipid-linked	767:778	arg1	glycans					780:786	lipid-linked glycans	767:786	lipid-linked glycans	767:786	We reasoned that lipid-linked glycans, which are intermediates directly used in glycan biosynthesis, would offer an alternative.					
31081628	11	30	theme	glycans	1792:1798	arg1	surrogates					1757:1766	surrogates	1757:1766	surrogates of natural lipid-linked glycans	1757:1798	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	0	31	theme	Bacterial	0:8	arg1	Wall					15:18	Bacterial Cell Wall	0:18	Bacterial Cell Wall	0:18	Bacterial Cell Wall Modification with a Glycolipid Substrate.					
31081628	10	32	theme	wall	1715:1718	arg1	biosynthesis					1720:1731	cell wall biosynthesis	1710:1731	cell wall biosynthesis	1710:1731	The lipid-based probe could also rescue wild-type cells treated with an inhibitor of cell wall biosynthesis.					
31081628	4	33	theme	nucleotide	731:740	arg1	sugars					742:747	nucleotide sugars	731:747	nucleotide sugars	731:747	We sought to develop a strategy that probes structures recalcitrant to metabolic incorporation and that complements approaches focused on nucleotide sugars.					
31081628	4	34	theme	recalcitrant	648:659	arg1	structures					637:646	structures	637:646	structures recalcitrant to metabolic incorporation	637:686	We sought to develop a strategy that probes structures recalcitrant to metabolic incorporation and that complements approaches focused on nucleotide sugars.					
31081628	11	35	theme	glycan	1928:1933	arg1	structure					1935:1943	glycan structure	1928:1943	glycan structure	1928:1943	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	8	36	theme	wall	1409:1412	arg1	characterization					1414:1429	cell wall characterization	1404:1429	cell wall characterization	1404:1429	The addition of an isotopically labeled glycan substrate facilitated cell wall characterization by NMR.					
31081628	2	37	theme	metabolic	218:226	arg1	incorporation					228:240	metabolic incorporation	218:240	metabolic incorporation	218:240	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	2	37	theme	metabolic	218:226	arg1	process					245:251	a process	243:251	a process in which non-natural sugars are taken up by cells	243:301	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	5	38	theme	glycan	830:835	arg1	biosynthesis					837:848	glycan biosynthesis	830:848	glycan biosynthesis	830:848	We reasoned that lipid-linked glycans, which are intermediates directly used in glycan biosynthesis, would offer an alternative.					
31081628	10	39	theme	lipid-based	1629:1639	arg1	probe					1641:1645	The lipid-based probe	1625:1645	The lipid-based probe	1625:1645	The lipid-based probe could also rescue wild-type cells treated with an inhibitor of cell wall biosynthesis.					
31081628	1	40	theme	glycans	101:107	arg1	importance					87:96	importance	87:96	importance	87:96	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	1	40	theme	glycans	101:107	arg1	ubiquity					74:81	ubiquity	74:81	ubiquity	74:81	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	9	41	theme	envelope	1615:1622	arg1	recovery					1594:1601	the full recovery	1585:1601	the full recovery of the cell envelope	1585:1622	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	7	42	theme	wall	1195:1198	arg1	arabinan					1200:1207	cell wall arabinan	1190:1207	cell wall arabinan	1190:1207	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	9	43	theme	Structural	1439:1448	arg1	analysis					1450:1457	Structural analysis	1439:1457	Structural analysis	1439:1457	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	3	44	theme	diverse	576:582	arg1	glycans					584:590	diverse glycans	576:590	diverse glycans	576:590	These studies have revealed that glycan intermediates can be shunted through multiple pathways, and this complexity can be heightened in bacteria, as they can catabolize diverse glycans.					
31081628	3	45	theme	glycan	439:444	arg1	intermediates					446:458	glycan intermediates	439:458	glycan intermediates	439:458	These studies have revealed that glycan intermediates can be shunted through multiple pathways, and this complexity can be heightened in bacteria, as they can catabolize diverse glycans.					
31081628	9	46	theme	arabinofuranosyl	1488:1503	arg1	transferases					1505:1516	all five known arabinofuranosyl transferases	1473:1516	all five known arabinofuranosyl transferases	1473:1516	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	0	47	with	Modification	20:31	arg1	Substrate					51:59	a Glycolipid Substrate	38:59	a Glycolipid Substrate	38:59	Bacterial Cell Wall Modification with a Glycolipid Substrate.					
31081628	2	48	theme	biosynthetic	383:394	arg1	pathways					396:403	biosynthetic pathways	383:403	biosynthetic pathways	383:403	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	10	49	theme	biosynthesis	1720:1731	arg1	inhibitor					1697:1705	an inhibitor	1694:1705	an inhibitor of cell wall biosynthesis	1694:1731	The lipid-based probe could also rescue wild-type cells treated with an inhibitor of cell wall biosynthesis.					
31081628	7	50	theme	C.	1084:1085	arg1	mutant					1098:1103	a C. glutamicum mutant	1082:1103	a C. glutamicum mutant that lacks arabinan	1082:1123	Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can serve as biosynthetic intermediates and function in chemical complementation.					
31081628	3	51	theme	multiple	483:490	arg1	pathways					492:499	multiple pathways	483:499	multiple pathways	483:499	These studies have revealed that glycan intermediates can be shunted through multiple pathways, and this complexity can be heightened in bacteria, as they can catabolize diverse glycans.					
31081628	0	52	theme	Glycolipid	40:49	arg1	Substrate					51:59	a Glycolipid Substrate	38:59	a Glycolipid Substrate	38:59	Bacterial Cell Wall Modification with a Glycolipid Substrate.					
31081628	11	53	theme	traditional	1828:1838	arg1	workflow					1840:1847	the cell's traditional workflow	1817:1847	the cell's traditional workflow	1817:1847	Our data indicate that surrogates of natural lipid-linked glycans can intervene in the cell's traditional workflow, indicating that biosynthetic incorporation is a powerful strategy for probing glycan structure and function.					
31081628	1	54	dep	ubiquity	74:81	arg1	the					70:72	the	70:72	the	70:72	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	8	55	theme	substrate	1382:1390	arg1	addition					1339:1346	The addition	1335:1346	The addition of an isotopically labeled glycan substrate	1335:1390	The addition of an isotopically labeled glycan substrate facilitated cell wall characterization by NMR.					
31081628	2	56	theme	Mammalian	177:185	arg1	glycans					187:193	Mammalian glycans	177:193	Mammalian glycans	177:193	Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up by cells, converted to nucleotide-sugar intermediates, and incorporated into glycans via biosynthetic pathways.					
31081628	8	57	theme	glycan	1375:1380	arg1	substrate					1382:1390	an isotopically labeled glycan substrate	1351:1390	an isotopically labeled glycan substrate	1351:1390	The addition of an isotopically labeled glycan substrate facilitated cell wall characterization by NMR.					
31081628	6	58	theme	tuberculosis	1062:1073	arg1	models					1038:1043	models	1038:1043	models of Mycobacterium tuberculosis	1038:1073	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	6	58	theme	tuberculosis	1062:1073	arg1	organisms					1014:1022	organisms	1014:1022	organisms that serve as models of Mycobacterium tuberculosis	1014:1073	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	9	59	theme	full	1589:1592	arg1	recovery					1594:1601	the full recovery	1585:1601	the full recovery of the cell envelope	1585:1622	Structural analysis revealed that all five known arabinofuranosyl transferases could process the exogenous lipid-linked sugar donor, allowing for the full recovery of the cell envelope.					
31081628	8	60	theme	cell	1404:1407	arg1	characterization					1414:1429	cell wall characterization	1404:1429	cell wall characterization	1404:1429	The addition of an isotopically labeled glycan substrate facilitated cell wall characterization by NMR.					
31081628	1	61	from	importance	87:96	arg1	biology					112:118	biology	112:118	biology	112:118	Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited.					
31081628	6	62	theme	Mycobacterium	1048:1060	arg1	tuberculosis					1062:1073	Mycobacterium tuberculosis	1048:1073	Mycobacterium tuberculosis	1048:1073	We generated synthetic arabinofuranosyl phospholipids to test this strategy in Corynebacterium glutamicum and Mycobacterium smegmatis, organisms that serve as models of Mycobacterium tuberculosis.					
31081628	10	63	theme	cell	1710:1713	arg1	biosynthesis					1720:1731	cell wall biosynthesis	1710:1731	cell wall biosynthesis	1710:1731	The lipid-based probe could also rescue wild-type cells treated with an inhibitor of cell wall biosynthesis.					
31081628	4	64	theme	metabolic	664:672	arg1	incorporation					674:686	metabolic incorporation	664:686	metabolic incorporation	664:686	We sought to develop a strategy that probes structures recalcitrant to metabolic incorporation and that complements approaches focused on nucleotide sugars.					
31539226	5	0	theme	patterns	962:969	arg1	alteration					936:945	the alteration	932:945	the alteration of sialylation patterns	932:969	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	0	1	theme	Urinary	87:93	arg1	Exosomes					95:102	Human Urinary Exosomes	81:102	Human Urinary Exosomes	81:102	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	3	2	theme	sulfated	528:535	arg1	species					537:543	sulfated species	528:543	sulfated species	528:543	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	7	3	theme	structural	1451:1460	arg1	isomers					1462:1468	26 structural isomers	1448:1468	26 structural isomers	1448:1468	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	7	3	theme	structural	1451:1460	arg1	compositions					1402:1413	175 compositions	1398:1413	175 compositions	1398:1413	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	4	4	theme	sulfated	884:891	arg1	compositions					902:913	N-glycan and sulfated N-glycan compositions	871:913	N-glycan and sulfated N-glycan compositions	871:913	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	3	5	theme	urinary	562:568	arg1	exosomes					570:577	urinary exosomes	562:577	urinary exosomes	562:577	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	4	6	theme	liquid	791:796	arg1	spectrometry					825:836	capillary liquid chromatography-tandem mass spectrometry	781:836	capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS)	781:847	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	6	theme	liquid	791:796	arg1	LC-MS/MS					839:846	LC-MS/MS	839:846	LC-MS/MS	839:846	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	5	7	theme	various	1005:1011	arg1	states					1021:1026	various disease states	1005:1026	various disease states	1005:1026	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	5	8	theme	acids	1169:1173	arg1	isomers					1151:1157	positional isomers	1140:1157	positional isomers of sialic acids	1140:1173	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	4	9	theme	mass	820:823	arg1	spectrometry					825:836	capillary liquid chromatography-tandem mass spectrometry	781:836	capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS)	781:847	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	9	theme	mass	820:823	arg1	LC-MS/MS					839:846	LC-MS/MS	839:846	LC-MS/MS	839:846	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	3	10	theme	N-glycan	495:502	arg1	compositions					504:515	N-glycan compositions	495:515	N-glycan compositions	495:515	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	3	10	theme	N-glycan	495:502	arg1	species					537:543	sulfated species	528:543	sulfated species	528:543	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	4	11	theme	mass	747:750	arg1	MALDI-MS					766:773	MALDI-MS	766:773	MALDI-MS	766:773	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	11	theme	mass	747:750	arg1	spectrometry					752:763	matrix-assisted laser desorption/ionization mass spectrometry	703:763	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	703:774	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	3	12	theme	compositions	504:515	arg1	species					537:543	sulfated species	528:543	sulfated species	528:543	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	3	12	theme	compositions	504:515	arg1	number					485:490	the number	481:490	the number of N-glycan compositions, including sulfated species, identified from urinary exosomes	481:577	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	4	13	theme	Capillary	650:658	arg1	spectrometry					681:692	Capillary electrophoresis-mass spectrometry	650:692	Capillary electrophoresis-mass spectrometry (CE-MS)	650:700	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	13	theme	Capillary	650:658	arg1	CE-MS					695:699	CE-MS	695:699	CE-MS	695:699	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	14	theme	matrix-assisted	703:717	arg1	MALDI-MS					766:773	MALDI-MS	766:773	MALDI-MS	766:773	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	14	theme	matrix-assisted	703:717	arg1	spectrometry					752:763	matrix-assisted laser desorption/ionization mass spectrometry	703:763	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	703:774	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	7	15	theme	sialic	1419:1424	arg1	compositions					1402:1413	175 compositions	1398:1413	175 compositions	1398:1413	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	7	15	theme	sialic	1419:1424	arg1	isomers					1439:1445	64 sialic acid linkage isomers	1416:1445	64 sialic acid linkage isomers	1416:1445	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	4	16	theme	N-glycan	893:900	arg1	compositions					902:913	N-glycan and sulfated N-glycan compositions	871:913	N-glycan and sulfated N-glycan compositions	871:913	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	2	17	theme	noninvasive	269:279	arg1	investigations					281:294	noninvasive investigations	269:294	noninvasive investigations into the pathophysiological states of the urinary system	269:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	1	18	theme	important	184:192	arg1	roles					205:209	their potentially important biological roles	166:209	their potentially important biological roles	166:209	The study of exosomes has become increasingly popular due to their potentially important biological roles.					
31539226	6	19	theme	α2-3	1250:1253	arg1	treatment					1265:1273	α2-3 sialidase treatment	1250:1273	α2-3 sialidase treatment	1250:1273	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	4	20	theme	capillary	781:789	arg1	spectrometry					825:836	capillary liquid chromatography-tandem mass spectrometry	781:836	capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS)	781:847	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	20	theme	capillary	781:789	arg1	LC-MS/MS					839:846	LC-MS/MS	839:846	LC-MS/MS	839:846	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	7	21	theme	219	1361:1363	arg1	structures					1374:1383	219 N-glycan structures	1361:1383	219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans	1361:1493	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	2	22	theme	exosomal	394:401	arg1	components					403:412	exosomal components	394:412	exosomal components	394:412	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	0	23	theme	In-Depth	0:7	arg1	Compositional					9:21	In-Depth Compositional	0:21	In-Depth Compositional	0:21	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	2	24	from	characterization	374:389	arg1	individuals					425:435	healthy individuals	417:435	healthy individuals	417:435	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	3	25	theme	sialic	597:602	arg1	linkages					609:616	the sialic acid linkages	593:616	the sialic acid linkages for many of those compositions	593:647	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	2	26	used	used	225:228	arg2	Urine					212:216	Urine	212:216	Urine	212:216	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	2	26	used	used	225:228	arg2	source					246:251	an effective source	233:251	an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system	233:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	0	27	theme	Structural	27:36	arg1	Characterization					38:53	Structural Characterization	27:53	Structural Characterization	27:53	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	7	28	theme	linkage	1431:1437	arg1	compositions					1402:1413	175 compositions	1398:1413	175 compositions	1398:1413	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	7	28	theme	linkage	1431:1437	arg1	isomers					1439:1445	64 sialic acid linkage isomers	1416:1445	64 sialic acid linkage isomers	1416:1445	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	4	29	theme	chromatography-tandem	798:818	arg1	spectrometry					825:836	capillary liquid chromatography-tandem mass spectrometry	781:836	capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS)	781:847	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	29	theme	chromatography-tandem	798:818	arg1	LC-MS/MS					839:846	LC-MS/MS	839:846	LC-MS/MS	839:846	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	30	theme	N-glycan	871:878	arg1	compositions					902:913	N-glycan and sulfated N-glycan compositions	871:913	N-glycan and sulfated N-glycan compositions	871:913	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	31	theme	electrophoresis-mass	660:679	arg1	spectrometry					681:692	Capillary electrophoresis-mass spectrometry	650:692	Capillary electrophoresis-mass spectrometry (CE-MS)	650:700	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	31	theme	electrophoresis-mass	660:679	arg1	CE-MS					695:699	CE-MS	695:699	CE-MS	695:699	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	5	32	theme	disease	1013:1019	arg1	states					1021:1026	various disease states	1005:1026	various disease states	1005:1026	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	6	33	theme	sialic	1279:1284	arg1	alkylamidation					1308:1321	sialic acid linkage-specific alkylamidation	1279:1321	sialic acid linkage-specific alkylamidation (SALSA)	1279:1329	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	6	33	theme	sialic	1279:1284	arg1	SALSA					1324:1328	SALSA	1324:1328	SALSA	1324:1328	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	5	34	theme	positional	1140:1149	arg1	isomers					1151:1157	positional isomers	1140:1157	positional isomers of sialic acids	1140:1173	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	2	35	theme	pathophysiological	305:322	arg1	states					324:329	the pathophysiological states	301:329	the pathophysiological states of the urinary system	301:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	4	36	used	used	854:857	arg2	spectrometry					752:763	matrix-assisted laser desorption/ionization mass spectrometry	703:763	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	703:774	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	36	used	used	854:857	arg2	CE-MS					695:699	CE-MS	695:699	CE-MS	695:699	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	36	used	used	854:857	arg2	MALDI-MS					766:773	MALDI-MS	766:773	MALDI-MS	766:773	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	36	used	used	854:857	arg2	spectrometry					825:836	capillary liquid chromatography-tandem mass spectrometry	781:836	capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS)	781:847	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	36	used	used	854:857	arg2	LC-MS/MS					839:846	LC-MS/MS	839:846	LC-MS/MS	839:846	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	36	used	used	854:857	arg2	spectrometry					681:692	Capillary electrophoresis-mass spectrometry	650:692	Capillary electrophoresis-mass spectrometry (CE-MS)	650:700	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	5	37	theme	ion-exchange	1029:1040	arg1	chromatography					1042:1055	ion-exchange chromatography	1029:1055	ion-exchange chromatography	1029:1055	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	2	38	theme	effective	236:244	arg1	Urine					212:216	Urine	212:216	Urine	212:216	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	2	38	theme	effective	236:244	arg1	source					246:251	an effective source	233:251	an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system	233:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	2	39	theme	healthy	417:423	arg1	individuals					425:435	healthy individuals	417:435	healthy individuals	417:435	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	5	40	theme	sialic	1162:1167	arg1	acids					1169:1173	sialic acids	1162:1173	sialic acids	1162:1173	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	3	41	theme	acid	604:607	arg1	linkages					609:616	the sialic acid linkages	593:616	the sialic acid linkages for many of those compositions	593:647	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	1	42	theme	exosomes	118:125	arg1	study					109:113	The study	105:113	The study of exosomes	105:125	The study of exosomes has become increasingly popular due to their potentially important biological roles.					
31539226	0	43	theme	N-Glycans	58:66	arg1	Compositional					9:21	In-Depth Compositional	0:21	In-Depth Compositional	0:21	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	0	43	theme	N-Glycans	58:66	arg1	Characterization					38:53	Structural Characterization	27:53	Structural Characterization	27:53	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	1	44	dep	important	184:192	arg1	biological					194:203	biological	194:203	biological	194:203	The study of exosomes has become increasingly popular due to their potentially important biological roles.					
31539226	7	45	theme	N-glycan	1365:1372	arg1	structures					1374:1383	219 N-glycan structures	1361:1383	219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans	1361:1493	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	6	46	theme	isomers	1209:1215	arg1	Structures					1176:1185	Structures	1176:1185	Structures of the sialyl-linkage isomers	1176:1215	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	5	47	theme	capillary	1071:1079	arg1	CE					1098:1099	CE	1098:1099	CE	1098:1099	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	5	47	theme	capillary	1071:1079	arg1	electrophoresis					1081:1095	microfluidic capillary electrophoresis	1058:1095	microfluidic capillary electrophoresis (CE)	1058:1100	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	3	48	theme	compositions	636:647	arg1	many					622:625	many	622:625	many	622:625	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	3	48	theme	compositions	636:647	arg1	compositions					636:647	those compositions	630:647	those compositions	630:647	Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions.					
31539226	4	49	theme	laser	719:723	arg1	MALDI-MS					766:773	MALDI-MS	766:773	MALDI-MS	766:773	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	49	theme	laser	719:723	arg1	spectrometry					752:763	matrix-assisted laser desorption/ionization mass spectrometry	703:763	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	703:774	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	2	50	theme	system	346:351	arg1	states					324:329	the pathophysiological states	301:329	the pathophysiological states of the urinary system	301:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	7	51	theme	sulfated	1478:1485	arg1	glycans					1487:1493	27 sulfated glycans	1475:1493	27 sulfated glycans	1475:1493	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	7	51	theme	sulfated	1478:1485	arg1	compositions					1402:1413	175 compositions	1398:1413	175 compositions	1398:1413	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	5	52	theme	microfluidic	1058:1069	arg1	CE					1098:1099	CE	1098:1099	CE	1098:1099	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	5	52	theme	microfluidic	1058:1069	arg1	electrophoresis					1081:1095	microfluidic capillary electrophoresis	1058:1095	microfluidic capillary electrophoresis (CE)	1058:1100	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	2	53	theme	exosomes	256:263	arg1	Urine					212:216	Urine	212:216	Urine	212:216	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	2	53	theme	exosomes	256:263	arg1	source					246:251	an effective source	233:251	an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system	233:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	2	54	theme	urinary	338:344	arg1	system					346:351	the urinary system	334:351	the urinary system	334:351	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	7	55	theme	acid	1426:1429	arg1	compositions					1402:1413	175 compositions	1398:1413	175 compositions	1398:1413	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	7	55	theme	acid	1426:1429	arg1	isomers					1439:1445	64 sialic acid linkage isomers	1416:1445	64 sialic acid linkage isomers	1416:1445	In total, we have identified 219 N-glycan structures that include 175 compositions, 64 sialic acid linkage isomers, 26 structural isomers, and 27 sulfated glycans.					
31539226	6	56	theme	sialyl-linkage	1194:1207	arg1	isomers					1209:1215	the sialyl-linkage isomers	1190:1215	the sialyl-linkage isomers	1190:1215	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	0	57	theme	Human	81:85	arg1	Exosomes					95:102	Human Urinary Exosomes	81:102	Human Urinary Exosomes	81:102	In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.					
31539226	5	58	theme	sialylation	950:960	arg1	patterns					962:969	sialylation patterns	950:969	sialylation patterns	950:969	Second, because the alteration of sialylation patterns has been previously implicated in various disease states, ion-exchange chromatography, microfluidic capillary electrophoresis (CE), and MALDI-MS were adopted to resolve positional isomers of sialic acids.					
31539226	6	59	theme	linkage-specific	1291:1306	arg1	alkylamidation					1308:1321	sialic acid linkage-specific alkylamidation	1279:1321	sialic acid linkage-specific alkylamidation (SALSA)	1279:1329	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	6	59	theme	linkage-specific	1291:1306	arg1	SALSA					1324:1328	SALSA	1324:1328	SALSA	1324:1328	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	6	60	theme	sialidase	1255:1263	arg1	treatment					1265:1273	α2-3 sialidase treatment	1250:1273	α2-3 sialidase treatment	1250:1273	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	4	61	theme	desorption/ionization	725:745	arg1	MALDI-MS					766:773	MALDI-MS	766:773	MALDI-MS	766:773	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	4	61	theme	desorption/ionization	725:745	arg1	spectrometry					752:763	matrix-assisted laser desorption/ionization mass spectrometry	703:763	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	703:774	Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan compositions.					
31539226	2	62	theme	components	403:412	arg1	characterization					374:389	detailed characterization	365:389	detailed characterization of exosomal components in healthy individuals	365:435	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31539226	6	63	theme	acid	1286:1289	arg1	alkylamidation					1308:1321	sialic acid linkage-specific alkylamidation	1279:1321	sialic acid linkage-specific alkylamidation (SALSA)	1279:1329	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	6	63	theme	acid	1286:1289	arg1	SALSA					1324:1328	SALSA	1324:1328	SALSA	1324:1328	Structures of the sialyl-linkage isomers were assigned indirectly through α2-3 sialidase treatment and sialic acid linkage-specific alkylamidation (SALSA).					
31539226	2	64	theme	detailed	365:372	arg1	characterization					374:389	detailed characterization	365:389	detailed characterization of exosomal components in healthy individuals	365:435	Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential.					
31998308	2	0	theme	IgG	347:349	arg1	glycovariants					351:363	IgG glycovariants	347:363	IgG glycovariants rich in terminal sialic acid residues	347:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	5	1	from	mice	951:954	arg1	deficient					924:932	deficient	924:932	deficient	924:932	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	2	theme	IgG	244:246	arg1	functions					257:265	IgG effector functions	244:265	IgG effector functions	244:265	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	2	3	gly	afucosylated	274:285	arg1	glycovariants					291:303	afucosylated IgG glycovariants	274:303	afucosylated IgG glycovariants	274:303	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	1	4	attach	attached	154:161	arg1	fragment					170:177	the fragment crystallizable	166:192	the fragment crystallizable of immunoglobulin G (IgG) antibodies	166:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	1	4	attach	attached	154:161	arg2	moiety					147:152	the biantennary sugar moiety	125:152	the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies	125:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	3	5	theme	IgG	546:548	arg1	secretion					550:558	IgG secretion	546:558	IgG secretion	546:558	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	5	6	theme	IgG	878:880	arg1	sialylation					889:899	human intravenous IgG (IVIg) sialylation	860:899	human intravenous IgG (IVIg) sialylation	860:899	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	7	theme	crystallizable	179:192	arg1	fragment					170:177	the fragment crystallizable	166:192	the fragment crystallizable of immunoglobulin G (IgG) antibodies	166:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	2	8	from	rich	365:368	arg1	residues					394:401	terminal sialic acid residues	373:401	terminal sialic acid residues	373:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	6	9	theme	plus	1127:1130	arg1	MS					1132:1133	plus MS	1127:1133	plus MS	1127:1133	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	6	9	theme	plus	1127:1130	arg1	HILIC-UPLC-FLR					1111:1124	HILIC-UPLC-FLR	1111:1124	HILIC-UPLC-FLR (plus MS)	1111:1134	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	2	10	theme	IgG	287:289	arg1	glycovariants					291:303	afucosylated IgG glycovariants	274:303	afucosylated IgG glycovariants	274:303	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	1	11	theme	immunoglobulin	197:210	arg1	IgG					215:217	IgG	215:217	IgG	215:217	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	1	11	theme	immunoglobulin	197:210	arg1	G					212:212	immunoglobulin G	197:212	immunoglobulin G (IgG) antibodies	197:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	2	12	theme	terminal	373:380	arg1	residues					394:401	terminal sialic acid residues	373:401	terminal sialic acid residues	373:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	4	13	theme	glycosylation	654:666	arg1	status					668:673	their glycosylation status	648:673	their glycosylation status	648:673	These findings raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity.					
31998308	5	14	theme	human	860:864	arg1	sialylation					889:899	human intravenous IgG (IVIg) sialylation	860:899	human intravenous IgG (IVIg) sialylation	860:899	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	3	15	link	linked	491:496	arg1	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	5	16	theme	linked	1026:1031	arg1	residues					1045:1052	α2,6 linked sialic acid residues	1021:1052	α2,6 linked sialic acid residues	1021:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	6	17	theme	IgG	1185:1187	arg1	glycosylation					1189:1201	therapeutic IgG glycosylation	1173:1201	therapeutic IgG glycosylation	1173:1201	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	5	18	theme	acid	1040:1043	arg1	residues					1045:1052	α2,6 linked sialic acid residues	1021:1052	α2,6 linked sialic acid residues	1021:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	19	link	linked	1026:1031	arg1	residues					1045:1052	α2,6 linked sialic acid residues	1021:1052	α2,6 linked sialic acid residues	1021:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	20	theme	extrinsic	760:768	arg1	processes					782:790	extrinsic sialylation processes	760:790	extrinsic sialylation processes	760:790	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	21	theme	sugar	141:145	arg1	moiety					147:152	the biantennary sugar moiety	125:152	the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies	125:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	0	22	theme	Anti-Inflammatory	62:78	arg1	Preparations					84:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	0	23	theme	Preparations	84:95	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	3	24	theme	α2,6	486:489	arg1	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	3	25	theme	recent	447:452	arg1	evidence					454:461	More recent evidence	442:461	More recent evidence	442:461	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	5	26	theme	B	753:753	arg1	cell					755:758	cell	755:758	cell	755:758	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	27	from	deficient	924:932	arg1	cells					939:943	B cells	937:943	B cells	937:943	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	27	from	deficient	924:932	arg1	mice					951:954	mice	951:954	mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues	951:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	2	28	theme	sialic	382:387	arg1	residues					394:401	terminal sialic acid residues	373:401	terminal sialic acid residues	373:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	0	29	theme	Minimal	0:6	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	7	30	theme	sialic	1290:1295	arg1	structures					1302:1311	sialic acid structures	1290:1311	sialic acid structures	1290:1311	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	3	31	theme	terminal	477:484	arg1	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	0	32	theme	Cell	10:13	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	7	33	theme	molecules	1271:1279	arg1	fraction					1255:1262	Only a very small fraction	1237:1262	Only a very small fraction of IgG molecules	1237:1279	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	0	34	theme	IgG	25:27	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	5	35	theme	therapeutic	799:809	arg1	preparations					815:826	therapeutic IgG preparations	799:826	therapeutic IgG preparations	799:826	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	0	36	theme	Pro-	53:56	arg1	Preparations					84:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	2	37	contain	have	305:308	arg1	glycovariants					291:303	afucosylated IgG glycovariants	274:303	afucosylated IgG glycovariants	274:303	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	2	37	contain	have	305:308	arg2	activity					329:336	enhanced cytotoxic activity	310:336	enhanced cytotoxic activity	310:336	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	5	38	theme	α2,6	1021:1024	arg1	residues					1045:1052	α2,6 linked sialic acid residues	1021:1052	α2,6 linked sialic acid residues	1021:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	3	39	attach	attached	518:525	arg1	antibodies					530:539	antibodies	530:539	antibodies post IgG secretion	530:558	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	3	39	attach	attached	518:525	arg2	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	7	40	theme	small	1249:1253	arg1	fraction					1255:1262	Only a very small fraction	1237:1262	Only a very small fraction of IgG molecules	1237:1279	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	3	41	theme	linked	491:496	arg1	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	6	42	theme	glycan	1090:1095	arg1	analysis					1097:1104	IgG glycan analysis	1086:1104	IgG glycan analysis	1086:1104	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	4	43	theme	therapeutic	606:616	arg1	antibodies					618:627	therapeutic antibodies	606:627	therapeutic antibodies	606:627	These findings raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity.					
31998308	5	44	from	cells	939:943	arg1	deficient					924:932	deficient	924:932	deficient	924:932	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	45	theme	antibodies	220:229	arg1	fragment					170:177	the fragment crystallizable	166:192	the fragment crystallizable of immunoglobulin G (IgG) antibodies	166:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	5	46	theme	sialylation	770:780	arg1	processes					782:790	extrinsic sialylation processes	760:790	extrinsic sialylation processes	760:790	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	6	47	theme	time	1071:1074	arg1	course					1076:1081	a time course	1069:1081	a time course of IgG glycan analysis	1069:1104	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	2	48	theme	afucosylated	274:285	arg1	glycovariants					291:303	afucosylated IgG glycovariants	274:303	afucosylated IgG glycovariants	274:303	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	1	49	theme	effector	248:255	arg1	functions					257:265	IgG effector functions	244:265	IgG effector functions	244:265	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	7	50	theme	therapeutic	1402:1412	arg1	glycosylation					1423:1435	therapeutic antibody glycosylation	1402:1435	therapeutic antibody glycosylation	1402:1435	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	5	51	theme	intravenous	866:876	arg1	sialylation					889:899	human intravenous IgG (IVIg) sialylation	860:899	human intravenous IgG (IVIg) sialylation	860:899	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	7	52	theme	antibody	1414:1421	arg1	glycosylation					1423:1435	therapeutic antibody glycosylation	1402:1435	therapeutic antibody glycosylation	1402:1435	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	2	53	theme	enhanced	310:317	arg1	activity					329:336	enhanced cytotoxic activity	310:336	enhanced cytotoxic activity	310:336	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	2	54	from	residues	394:401	arg1	rich					365:368	rich	365:368	rich	365:368	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	5	55	theme	IVIg	883:886	arg1	sialylation					889:899	human intravenous IgG (IVIg) sialylation	860:899	human intravenous IgG (IVIg) sialylation	860:899	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	56	theme	G	212:212	arg1	antibodies					220:229	immunoglobulin G (IgG) antibodies	197:229	immunoglobulin G (IgG) antibodies	197:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	1	57	theme	Select	106:111	arg1	residues					113:120	Select residues	106:120	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies	106:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	5	58	theme	sialic	1033:1038	arg1	residues					1045:1052	α2,6 linked sialic acid residues	1021:1052	α2,6 linked sialic acid residues	1021:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	59	from	changes	849:855	arg1	sialylation					889:899	human intravenous IgG (IVIg) sialylation	860:899	human intravenous IgG (IVIg) sialylation	860:899	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	5	60	theme	deficient	924:932	arg1	mice					919:922	mice	919:922	mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues	919:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	6	61	theme	therapeutic	1173:1183	arg1	glycosylation					1189:1201	therapeutic IgG glycosylation	1173:1201	therapeutic IgG glycosylation	1173:1201	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	5	62	theme	residues	1045:1052	arg1	addition					1009:1016	the addition	1005:1016	the addition of α2,6 linked sialic acid residues	1005:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	1	63	theme	biantennary	129:139	arg1	moiety					147:152	the biantennary sugar moiety	125:152	the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies	125:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	0	64	theme	IgG	80:82	arg1	Preparations					84:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	5	65	from	injection	906:914	arg1	mice					919:922	mice	919:922	mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues	919:1052	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	2	66	theme	cytotoxic	319:327	arg1	activity					329:336	enhanced cytotoxic activity	310:336	enhanced cytotoxic activity	310:336	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	5	67	theme	extent	746:751	arg1	cell					755:758	cell	755:758	cell	755:758	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	4	68	dep	raise	576:580	arg1	concerns					582:589	concerns	582:589	raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity	576:720	These findings raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity.					
31998308	7	69	theme	acid	1297:1300	arg1	structures					1302:1311	sialic acid structures	1290:1311	sialic acid structures	1290:1311	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	2	70	theme	acid	389:392	arg1	residues					394:401	terminal sialic acid residues	373:401	terminal sialic acid residues	373:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	0	71	theme	B	8:8	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	5	72	theme	B	937:937	arg1	cells					939:943	B cells	937:943	B cells	937:943	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	0	73	mod	Modifications	36:48	arg3	Cell					10:13	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	0	73	mod	Modifications	36:48	arg3	B					8:8	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	0	73	mod	Modifications	36:48	arg3	IgG					25:27	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	0	73	mod	Modifications	36:48	arg1	Preparations					84:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Pro- and Anti-Inflammatory IgG Preparations	53:95	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	0	73	mod	Modifications	36:48	arg3	Glycan					29:34	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	3	74	theme	More	442:445	arg1	evidence					454:461	More recent evidence	442:461	More recent evidence	442:461	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	0	75	theme	Extrinsic	15:23	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	1	76	from	residues	113:120	arg1	moiety					147:152	the biantennary sugar moiety	125:152	the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies	125:229	Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions.					
31998308	7	77	theme	IgG	1267:1269	arg1	molecules					1271:1279	IgG molecules	1267:1279	IgG molecules	1267:1279	Only a very small fraction of IgG molecules acquired sialic acid structures predominantly in the Fab- but not the Fc-portion upon injection in vivo, suggesting that therapeutic antibody glycosylation will remain stable upon injection in vivo.					
31998308	2	78	theme	anti-inflammatory	415:431	arg1	effects					433:439	anti-inflammatory effects	415:439	anti-inflammatory effects	415:439	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31998308	0	79	theme	Glycan	29:34	arg1	Modifications					36:48	Minimal B Cell Extrinsic IgG Glycan Modifications	0:48	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo	0:103	Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo.					
31998308	5	80	theme	IgG	811:813	arg1	preparations					815:826	therapeutic IgG preparations	799:826	therapeutic IgG preparations	799:826	To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations in vivo, we analyzed changes in human intravenous IgG (IVIg) sialylation upon injection in mice deficient in B cells or in mice lacking the sialyltransferase 1, which catalyzes the addition of α2,6 linked sialic acid residues.					
31998308	6	81	theme	analysis	1097:1104	arg1	course					1076:1081	a time course	1069:1081	a time course of IgG glycan analysis	1069:1104	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	4	82	theme	antibodies	618:627	arg1	use					599:601	the use	595:601	the use of therapeutic antibodies	595:627	These findings raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity.					
31998308	3	83	theme	sialic	498:503	arg1	acids					505:509	terminal α2,6 linked sialic acids	477:509	terminal α2,6 linked sialic acids	477:509	More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion.					
31998308	6	84	theme	IgG	1086:1088	arg1	analysis					1097:1104	IgG glycan analysis	1086:1104	IgG glycan analysis	1086:1104	By performing a time course of IgG glycan analysis with HILIC-UPLC-FLR (plus MS) and xCGE-LIF our study suggests that therapeutic IgG glycosylation is stable upon injection in vivo.					
31998308	2	85	theme	rich	365:368	arg1	glycovariants					351:363	IgG glycovariants	347:363	IgG glycovariants rich in terminal sialic acid residues	347:401	Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects.					
31608555	4	0	theme	resolution	856:865	arg1	range					867:871	a resolution range	854:871	a resolution range of 1.6-2.0 Å	854:884	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	1	with	complex	717:723	arg1	α-glycosides					743:754	disaccharide α-glycosides	730:754	disaccharide α-glycosides	730:754	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	8	2	dep	along	1463:1467	arg1	with					1469:1472	with	1469:1472	with	1469:1472	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	6	3	theme	glycosyl	1147:1154	arg1	unit					1156:1159	the glucose and glycosyl unit	1131:1159	unit	1156:1159	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	6	4	theme	glucose	1135:1141	arg1	unit					1156:1159	the glucose and glycosyl unit	1131:1159	unit	1156:1159	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	9	5	theme	transmembrane	1855:1867	arg1	TMD					1877:1879	TMD	1877:1879	TMD	1877:1879	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	5	theme	transmembrane	1855:1867	arg1	domain					1869:1874	the transmembrane domain	1851:1874	the transmembrane domain (TMD)	1851:1880	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	2	6	dep	inside	290:295	arg1	achieved					309:316	achieved	309:316	inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system	290:408	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	4	7	dep	αGlyBP	658:663	arg1	TTHA0356					674:681	TTHA0356	674:681	TTHA0356	674:681	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	5	8	dep	linkage	993:999	arg1	both					977:980	both	977:980	both	977:980	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	2	9	theme	Selective	249:257	arg1	acquisition					259:269	Selective acquisition	249:269	Selective acquisition of these molecules	249:288	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	1	10	theme	epimeric	233:240	arg1	state					242:246	epimeric state	233:246	epimeric state	233:246	Carbohydrate (or sugar) molecules are extremely diverse regarding their length, linkage and epimeric state.					
31608555	9	11	theme	domain	1686:1691	arg1	movement					1699:1706	the N-terminal domain (NTD) movement	1671:1706	the N-terminal domain (NTD) movement	1671:1706	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	8	12	theme	open	1423:1426	arg1	conformations					1439:1451	open and closed conformations	1423:1451	open and closed conformations of αGlyBP	1423:1461	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	7	13	theme	disaccharide	1333:1344	arg1	α-glycosides					1346:1357	disaccharide α-glycosides	1333:1357	disaccharide α-glycosides	1333:1357	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	7	13	theme	disaccharide	1333:1344	arg1	s					1329:1329	the high-affinity ligand(s)	1304:1330	the high-affinity ligand(s) (disaccharide α-glycosides)	1304:1358	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	9	14	theme	substrate	1889:1897	arg1	translocation					1899:1911	substrate translocation	1889:1911	substrate translocation	1889:1911	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	4	15	theme	disaccharide	730:741	arg1	α-glycosides					743:754	disaccharide α-glycosides	730:754	disaccharide α-glycosides	730:754	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	5	16	theme	hydroxyl	1024:1031	arg1	group					1033:1037	an epimeric hydroxyl group	1012:1037	an epimeric hydroxyl group	1012:1037	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	9	17	theme	ligand	1621:1626	arg1	encapsulation					1604:1616	encapsulation	1604:1616	encapsulation of ligand inside the active site	1604:1649	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	8	18	theme	closed	1432:1437	arg1	conformations					1439:1451	open and closed conformations	1423:1451	open and closed conformations of αGlyBP	1423:1461	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	10	19	theme	RCSB	1957:1960	arg1	Bank					1975:1978	RCSB Protein Data Bank	1957:1978	RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE	1957:2124	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	4	20	dep	protein	649:655	arg1	ID					670:671	ORF ID	666:671	ORF ID	666:671	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	20	dep	protein	649:655	arg1	α-1,1					774:778	α-1,1	774:778	α-1,1	774:778	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	20	dep	protein	649:655	arg1	trehalose					763:771	trehalose	763:771	an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1)	626:779	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	20	dep	protein	649:655	arg1	αGlyBP					658:663	αGlyBP	658:663	αGlyBP	658:663	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	2	21	theme	molecules	280:288	arg1	acquisition					259:269	Selective acquisition	249:269	Selective acquisition of these molecules	249:288	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	1	22	theme	sugar	158:162	arg1	molecules					165:173	Carbohydrate (or sugar) molecules	141:173	molecules	165:173	Carbohydrate (or sugar) molecules are extremely diverse regarding their length, linkage and epimeric state.					
31608555	6	23	theme	α-glycosides	1177:1188	arg1	unit					1156:1159	the glucose and glycosyl unit	1131:1159	unit	1156:1159	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	0	24	theme	disaccharide	90:101	arg1	α-glycosides					103:114	disaccharide α-glycosides	90:114	disaccharide α-glycosides	90:114	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	9	25	theme	C-terminal	1721:1730	arg1	αGlyBP					1748:1753	αGlyBP	1748:1753	αGlyBP	1748:1753	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	25	theme	C-terminal	1721:1730	arg1	domain					1732:1737	the C-terminal domain	1717:1737	the C-terminal domain (CTD) of αGlyBP	1717:1753	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	25	theme	C-terminal	1721:1730	arg1	rigid					1775:1779	rigid	1775:1779	rigid	1775:1779	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	25	theme	C-terminal	1721:1730	arg1	responsible					1802:1812	responsible	1802:1812	responsible	1802:1812	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	25	theme	C-terminal	1721:1730	arg1	CTD					1740:1742	CTD	1740:1742	CTD	1740:1742	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	8	26	theme	mutagenic	1474:1482	arg1	studies					1502:1508	mutagenic and thermodynamic studies	1474:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies	1397:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	10	27	theme	Data	1970:1973	arg1	Bank					1975:1978	RCSB Protein Data Bank	1957:1978	RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE	1957:2124	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	8	28	theme	thermodynamic	1488:1500	arg1	studies					1502:1508	mutagenic and thermodynamic studies	1474:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies	1397:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	2	29	theme	transport	393:401	arg1	system					403:408	ATP-binding cassette (ABC) transport system	366:408	ATP-binding cassette (ABC) transport system	366:408	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	7	30	theme	ligand	1322:1327	arg1	α-glycosides					1346:1357	disaccharide α-glycosides	1333:1357	disaccharide α-glycosides	1333:1357	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	7	30	theme	ligand	1322:1327	arg1	s					1329:1329	the high-affinity ligand(s)	1304:1330	the high-affinity ligand(s) (disaccharide α-glycosides)	1304:1358	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	0	31	theme	Structural	0:9	arg1	correlation					29:39	Structural and thermodynamic correlation	0:39	Structural and thermodynamic correlation	0:39	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	5	32	theme	sugars	926:931	arg1	types					917:921	multiple types	908:921	multiple types of sugars	908:931	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	9	33	theme	αGlyBP	1748:1753	arg1	αGlyBP					1748:1753	αGlyBP	1748:1753	αGlyBP	1748:1753	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	33	theme	αGlyBP	1748:1753	arg1	domain					1732:1737	the C-terminal domain	1717:1737	the C-terminal domain (CTD) of αGlyBP	1717:1753	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	33	theme	αGlyBP	1748:1753	arg1	rigid					1775:1779	rigid	1775:1779	rigid	1775:1779	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	33	theme	αGlyBP	1748:1753	arg1	responsible					1802:1812	responsible	1802:1812	responsible	1802:1812	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	33	theme	αGlyBP	1748:1753	arg1	CTD					1740:1742	CTD	1740:1742	CTD	1740:1742	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	8	34	theme	hinge	1525:1529	arg1	site					1563:1566	the first interaction site	1541:1566	the first interaction site for the ligands	1541:1582	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	8	34	theme	hinge	1525:1529	arg1	region					1531:1536	the hinge region	1521:1536	the hinge region	1521:1536	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	0	35	theme	thermodynamic	15:27	arg1	correlation					29:39	Structural and thermodynamic correlation	0:39	Structural and thermodynamic correlation	0:39	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	9	36	dep	achieved	1654:1661	arg1	whereas					1709:1715	whereas	1709:1715	whereas	1709:1715	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	8	37	theme	first	1545:1549	arg1	site					1563:1566	the first interaction site	1541:1566	the first interaction site for the ligands	1541:1582	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	8	37	theme	first	1545:1549	arg1	region					1531:1536	the hinge region	1521:1536	the hinge region	1521:1536	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	2	38	theme	-binding	346:353	arg1	protein					355:361	the substrate (or solute)-binding protein	321:361	protein	355:361	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	0	39	theme	selective	57:65	arg1	mechanism					77:85	the selective transport mechanism	53:85	the selective transport mechanism of disaccharide α-glycosides	53:114	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	5	40	theme	epimeric	1015:1022	arg1	group					1033:1037	an epimeric hydroxyl group	1012:1037	an epimeric hydroxyl group	1012:1037	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	6	41	theme	subsite	1102:1108	arg1	B					1110:1110	subsite B	1102:1110	subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides	1102:1188	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	2	42	theme	solute	339:344	arg1	protein					355:361	the substrate (or solute)-binding protein	321:361	protein	355:361	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	10	43	from	Bank	1975:1978	arg1	available					1944:1952	available	1944:1952	available	1944:1952	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	43	from	Bank	1975:1978	arg1	DATABASE					1914:1921	DATABASE	1914:1921	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.	1914:2125	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	4	44	dep	Thermus	688:694	arg1	thermophilus					696:707	thermophilus	696:707	thermophilus	696:707	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	7	45	theme	paradoxical	1253:1263	arg1	behavior					1265:1272	the paradoxical behavior	1249:1272	the paradoxical behavior of glucose	1249:1283	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	10	46	theme	accession	1990:1998	arg1	number					2000:2005	the accession number	1986:2005	the accession number	1986:2005	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	46	theme	accession	1990:1998	arg1	s					2007:2007	s	2007:2007	s	2007:2007	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	4	47	theme	α-glycoside-binding	629:647	arg1	protein					649:655	an α-glycoside-binding protein	626:655	an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1)	626:779	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	3	48	theme	selective	459:467	arg1	transport					469:477	the selective transport	455:477	the selective transport of diverse carbohydrates	455:502	However, the molecular mechanism underlying the selective transport of diverse carbohydrates remains unclear mainly owing to their structural complexity and stereochemistry.					
31608555	8	49	theme	Comparative	1397:1407	arg1	assessment					1409:1418	Comparative assessment	1397:1418	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies	1397:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	2	50	theme	cassette	378:385	arg1	system					403:408	ATP-binding cassette (ABC) transport system	366:408	ATP-binding cassette (ABC) transport system	366:408	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	4	51	from	protein	649:655	arg1	complex					717:723	complex	717:723	complex with disaccharide α-glycosides	717:754	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	7	52	theme	structural	1224:1233	arg1	data					1235:1238	structural data	1224:1238	structural data	1224:1238	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	4	53	theme	crystal	604:610	arg1	maltose					799:805	maltose	799:805	maltose (α-1,4)	799:813	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	53	theme	crystal	604:610	arg1	sucrose					782:788	sucrose	782:788	sucrose (α-1,2)	782:796	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	53	theme	crystal	604:610	arg1	structures					612:621	crystal structures	604:621	crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1)	604:779	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	53	theme	crystal	604:610	arg1	glucose					839:845	glucose	839:845	glucose	839:845	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	53	theme	crystal	604:610	arg1	palatinose					816:825	palatinose	816:825	palatinose (α-1,6)	816:833	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	3	54	theme	structural	542:551	arg1	complexity					553:562	their structural complexity	536:562	their structural complexity	536:562	However, the molecular mechanism underlying the selective transport of diverse carbohydrates remains unclear mainly owing to their structural complexity and stereochemistry.					
31608555	9	55	theme	active	1639:1644	arg1	site					1646:1649	the active site	1635:1649	the active site	1635:1649	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	7	56	theme	protein	1388:1394	arg1	site					1376:1379	the active site	1365:1379	the active site of the protein	1365:1394	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	7	56	theme	protein	1388:1394	arg1	protein					1388:1394	the protein	1384:1394	the protein	1384:1394	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	4	57	theme	Thermus	688:694	arg1	HB8					709:711	Thermus thermophilus HB8	688:711	Thermus thermophilus HB8	688:711	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	7	58	theme	active	1369:1374	arg1	site					1376:1379	the active site	1365:1379	the active site of the protein	1365:1394	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	7	58	theme	active	1369:1374	arg1	protein					1388:1394	the protein	1384:1394	the protein	1384:1394	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	9	59	theme	N-terminal	1675:1684	arg1	NTD					1694:1696	NTD	1694:1696	NTD	1694:1696	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	59	theme	N-terminal	1675:1684	arg1	domain					1686:1691	the N-terminal domain	1671:1691	the N-terminal domain (NTD) movement	1671:1706	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	10	60	theme	Structural	1924:1933	arg1	data					1935:1938	Structural data	1924:1938	Structural data	1924:1938	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	8	61	theme	conformations	1439:1451	arg1	assessment					1409:1418	Comparative assessment	1397:1418	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies	1397:1508	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	5	62	theme	multiple	908:915	arg1	types					917:921	multiple types	908:921	multiple types of sugars	908:931	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	4	63	theme	1.6-2.0 Å	876:884	arg1	range					867:871	a resolution range	854:871	a resolution range of 1.6-2.0 Å	854:884	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	8	64	theme	αGlyBP	1456:1461	arg1	conformations					1439:1451	open and closed conformations	1423:1451	open and closed conformations of αGlyBP	1423:1461	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	3	65	theme	diverse	482:488	arg1	carbohydrates					490:502	diverse carbohydrates	482:502	diverse carbohydrates	482:502	However, the molecular mechanism underlying the selective transport of diverse carbohydrates remains unclear mainly owing to their structural complexity and stereochemistry.					
31608555	4	66	theme	ORF	666:668	arg1	ID					670:671	ORF ID	666:671	ORF ID	666:671	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	66	theme	ORF	666:668	arg1	αGlyBP					658:663	αGlyBP	658:663	αGlyBP	658:663	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	6	67	theme	disaccharide	1164:1175	arg1	α-glycosides					1177:1188	disaccharide α-glycosides	1164:1188	disaccharide α-glycosides	1164:1188	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	0	68	theme	α-glycosides	103:114	arg1	mechanism					77:85	the selective transport mechanism	53:85	the selective transport mechanism of disaccharide α-glycosides	53:114	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAH					2034:2037	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAH	2034:2037	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAM					2052:2055	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAM	2052:2055	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6J9Y					2016:2019	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6J9Y	2016:2019	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAN					2058:2061	6JAN	2058:2061	6JAN	2058:2061	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAD					2022:2025	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAD	2022:2025	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAI					2040:2043	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAI	2040:2043	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAL					2046:2049	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAL	2046:2049	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	10	69	dep	6JAO	2064:2067	arg1	6JAG					2028:2031	6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO	2010:2067	6JAG	2028:2031	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	0	70	theme	ABC	124:126	arg1	transporter					128:138	ABC transporter	124:138	ABC transporter	124:138	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	5	71	theme	glycosidic	982:991	arg1	linkage					993:999	glycosidic linkage	982:999	glycosidic linkage	982:999	Despite transporting multiple types of sugars, αGlyBP maintains its stereoselectivity for both glycosidic linkage as well as an epimeric hydroxyl group.					
31608555	10	72	theme	Protein	1962:1968	arg1	Bank					1975:1978	RCSB Protein Data Bank	1957:1978	RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE	1957:2124	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	4	73	from	structures	612:621	arg1	complex					717:723	complex	717:723	complex with disaccharide α-glycosides	717:754	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	7	74	theme	high-affinity	1308:1320	arg1	α-glycosides					1346:1357	disaccharide α-glycosides	1333:1357	disaccharide α-glycosides	1333:1357	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	7	74	theme	high-affinity	1308:1320	arg1	s					1329:1329	the high-affinity ligand(s)	1304:1330	the high-affinity ligand(s) (disaccharide α-glycosides)	1304:1358	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	9	75	theme	other	1592:1596	arg1	hand					1598:1601	the other hand	1588:1601	the other hand	1588:1601	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	3	76	theme	molecular	424:432	arg1	mechanism					434:442	the molecular mechanism	420:442	the molecular mechanism underlying the selective transport of diverse carbohydrates	420:502	However, the molecular mechanism underlying the selective transport of diverse carbohydrates remains unclear mainly owing to their structural complexity and stereochemistry.					
31608555	10	77	from	available	1944:1952	arg1	Bank					1975:1978	RCSB Protein Data Bank	1957:1978	RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE	1957:2124	DATABASE: Structural data are available in RCSB Protein Data Bank under the accession number(s) 6J9W, 6J9Y, 6JAD, 6JAG, 6JAH, 6JAI, 6JAL, 6JAM, 6JAN, 6JAO, 6JAP, 6JAQ, 6JAR, 6JAZ, 6JB0, 6JB4, 6JBA, 6JBB and 6JBE.					
31608555	7	78	theme	glucose	1277:1283	arg1	behavior					1265:1272	the paradoxical behavior	1249:1272	the paradoxical behavior of glucose	1249:1283	In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein.					
31608555	8	79	theme	interaction	1551:1561	arg1	site					1563:1566	the first interaction site	1541:1566	the first interaction site for the ligands	1541:1582	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	8	79	theme	interaction	1551:1561	arg1	region					1531:1536	the hinge region	1521:1536	the hinge region	1521:1536	Comparative assessment of open and closed conformations of αGlyBP along with mutagenic and thermodynamic studies identifies the hinge region as the first interaction site for the ligands.					
31608555	2	80	theme	system	403:408	arg1	substrate					325:333	the substrate (or solute)-binding protein	321:361	substrate	325:333	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	2	80	theme	system	403:408	arg1	protein					355:361	the substrate (or solute)-binding protein	321:361	protein	355:361	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	0	81	theme	transport	67:75	arg1	mechanism					77:85	the selective transport mechanism	53:85	the selective transport mechanism of disaccharide α-glycosides	53:114	Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.					
31608555	4	82	theme	protein	649:655	arg1	maltose					799:805	maltose	799:805	maltose (α-1,4)	799:813	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	82	theme	protein	649:655	arg1	sucrose					782:788	sucrose	782:788	sucrose (α-1,2)	782:796	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	82	theme	protein	649:655	arg1	structures					612:621	crystal structures	604:621	crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1)	604:779	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	82	theme	protein	649:655	arg1	glucose					839:845	glucose	839:845	glucose	839:845	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	82	theme	protein	649:655	arg1	palatinose					816:825	palatinose	816:825	palatinose (α-1,6)	816:833	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	9	83	with	interaction	1834:1844	arg1	TMD					1877:1879	TMD	1877:1879	TMD	1877:1879	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	9	83	with	interaction	1834:1844	arg1	domain					1869:1874	the transmembrane domain	1851:1874	the transmembrane domain (TMD)	1851:1880	On the other hand, encapsulation of ligand inside the active site is achieved through the N-terminal domain (NTD) movement, whereas the C-terminal domain (CTD) of αGlyBP is identified to be rigid and postulated to be responsible for maintaining the interaction with the transmembrane domain (TMD) during substrate translocation.					
31608555	3	84	theme	carbohydrates	490:502	arg1	transport					469:477	the selective transport	455:477	the selective transport of diverse carbohydrates	455:502	However, the molecular mechanism underlying the selective transport of diverse carbohydrates remains unclear mainly owing to their structural complexity and stereochemistry.					
31608555	6	85	theme	active-site	1082:1092	arg1	pocket					1094:1099	the active-site pocket	1078:1099	the active-site pocket	1078:1099	Out of the two subsites identified in the active-site pocket, subsite B which accommodates the glucose and glycosyl unit of disaccharide α-glycosides is highly conserved.					
31608555	2	86	theme	ATP-binding	366:376	arg1	ABC					388:390	ABC	388:390	ABC	388:390	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	2	86	theme	ATP-binding	366:376	arg1	cassette					378:385	ATP-binding cassette	366:385	ATP-binding cassette (ABC) transport system	366:408	Selective acquisition of these molecules inside the cell is achieved by the substrate (or solute)-binding protein of ATP-binding cassette (ABC) transport system.					
31608555	4	87	from	complex	717:723	arg1	maltose					799:805	maltose	799:805	maltose (α-1,4)	799:813	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	87	from	complex	717:723	arg1	sucrose					782:788	sucrose	782:788	sucrose (α-1,2)	782:796	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	87	from	complex	717:723	arg1	structures					612:621	crystal structures	604:621	crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1)	604:779	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	87	from	complex	717:723	arg1	glucose					839:845	glucose	839:845	glucose	839:845	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
31608555	4	87	from	complex	717:723	arg1	palatinose					816:825	palatinose	816:825	palatinose (α-1,6)	816:833	This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å.					
29317495	9	0	theme	glycoside	1631:1639	arg1	class					1655:1659	a new glycoside phosphorylase class	1625:1659	a new glycoside phosphorylase class	1625:1659	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	1	1	contain	have	139:142	arg1	phosphorylases					124:137	Glycoside phosphorylases	114:137	Glycoside phosphorylases	114:137	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	1	1	contain	have	139:142	arg2	potential					157:165	considerable potential	144:165	considerable potential	144:165	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	1	1	contain	have	139:142	arg2	catalysts					170:178	catalysts	170:178	catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials	170:290	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	6	2	theme	active	1195:1200	arg1	site					1202:1205	the active site	1191:1205	the active site important for substrate recognition	1191:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	9	3	contain	has	1769:1771	arg1	which					1763:1767	which	1763:1767	which	1763:1767	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	9	3	contain	has	1769:1771	arg2	implications					1773:1784	implications	1773:1784	implications for biotechnological applications	1773:1818	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	1	4	theme	novel	276:280	arg1	materials					282:290	novel materials	276:290	novel materials	276:290	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	2	5	theme	substrate	306:314	arg1	small					379:383	small	379:383	small	379:383	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	2	5	theme	substrate	306:314	arg1	diversity					316:324	the substrate diversity	302:324	the substrate diversity of currently identified phosphorylases	302:363	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	8	6	theme	metagenomics	1552:1563	arg1	library					1565:1571	a metagenomics library	1550:1571	a metagenomics library	1550:1571	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	6	7	theme	X-ray	1067:1071	arg1	analysis					1090:1097	X-ray crystallographic analysis	1067:1097	X-ray crystallographic analysis	1067:1097	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	0	8	theme	metagenomic	93:103	arg1	library					105:111	a metagenomic library	91:111	a metagenomic library	91:111	Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.					
29317495	9	9	theme	phosphorylase	1641:1653	arg1	class					1655:1659	a new glycoside phosphorylase class	1625:1659	a new glycoside phosphorylase class	1625:1659	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	4	10	theme	gene	820:823	arg1	bglP					825:828	the gene bglP	816:828	the gene bglP	816:828	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	6	11	from	residue	1180:1186	arg1	site					1202:1205	the active site	1191:1205	the active site important for substrate recognition	1191:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	3	12	theme	glycoside	614:622	arg1	activity					638:645	glycoside phosphorylase activity	614:645	glycoside phosphorylase activity	614:645	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	3	13	theme	2,4-dinitrophenyl	533:549	arg1	β-d-glucoside					551:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside	509:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc)	509:572	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	3	13	theme	2,4-dinitrophenyl	533:549	arg1	DNPGlc					566:571	DNPGlc	566:571	DNPGlc	566:571	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	8	14	theme	active	1529:1534	arg1	clones					1536:1541	active clones	1529:1541	active clones	1529:1541	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	8	15	attach	present	1375:1381	arg2	we					1372:1373	we	1372:1373	we	1372:1373	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	8	15	attach	present	1375:1381	arg1	summary					1363:1369	summary	1363:1369	summary	1363:1369	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	5	16	theme	glycosyl-enzyme	1037:1051	arg1	intermediate					1053:1064	a covalent glycosyl-enzyme intermediate	1026:1064	a covalent glycosyl-enzyme intermediate	1026:1064	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	6	17	theme	key	1166:1168	arg1	residue					1180:1186	a key glutamine residue	1164:1186	a key glutamine residue in the active site important for substrate recognition	1164:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	3	18	theme	high-throughput	468:482	arg1	approach					494:501	a high-throughput screening approach	466:501	a high-throughput screening approach	466:501	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	7	19	theme	substrate	1297:1305	arg1	specificity					1307:1317	the substrate specificity	1293:1317	the substrate specificity from glucoside to N-acetylglucosaminide	1293:1357	Substituting this glutamine for a serine swapped the substrate specificity from glucoside to N-acetylglucosaminide.					
29317495	5	20	theme	displacement	983:994	arg1	mechanism					1006:1014	a double displacement ping-pong mechanism	974:1014	a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate	974:1064	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	8	21	theme	high-throughput	1385:1399	arg1	approach					1411:1418	a high-throughput screening approach	1383:1418	a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library	1383:1571	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	3	22	theme	screening	484:492	arg1	approach					494:501	a high-throughput screening approach	466:501	a high-throughput screening approach	466:501	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	4	23	from	family	896:901	arg1	phosphorylase					864:876	a retaining β-glycoside phosphorylase	840:876	a retaining β-glycoside phosphorylase from the CAZy GH3 family	840:901	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	2	24	theme	identified	339:348	arg1	phosphorylases					350:363	currently identified phosphorylases	329:363	currently identified phosphorylases	329:363	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	1	25	theme	useful	200:205	arg1	glycans					207:213	useful glycans	200:213	useful glycans for products ranging from functional foods and prebiotics to novel materials	200:290	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	0	26	theme	Structural	0:9	arg1	analysis					27:34	Structural and mechanistic analysis	0:34	Structural and mechanistic analysis of a β-glycoside phosphorylase	0:65	Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.					
29317495	9	27	theme	simple	1695:1700	arg1	ways					1702:1705	devising simple ways	1686:1705	devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications	1686:1818	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	5	28	theme	mechanistic	916:926	arg1	analysis					928:935	Kinetic and mechanistic analysis	904:935	Kinetic and mechanistic analysis of the gene product, BglP,	904:962	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	4	29	theme	phosphate	794:802	arg1	presence					782:789	the presence	778:789	the presence of phosphate	778:802	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	1	30	theme	glycans	207:213	arg1	assembly					188:195	the assembly	184:195	the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials	184:290	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	8	31	theme	β-glycoside	1436:1446	arg1	phosphorylases					1448:1461	β-glycoside phosphorylases	1436:1461	β-glycoside phosphorylases	1436:1461	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	5	32	theme	double	976:981	arg1	mechanism					1006:1014	a double displacement ping-pong mechanism	974:1014	a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate	974:1064	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	3	33	theme	phosphorylase	624:636	arg1	activity					638:645	glycoside phosphorylase activity	614:645	glycoside phosphorylase activity	614:645	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	9	34	theme	screen	1597:1602	arg1	Implementation					1574:1587	Implementation	1574:1587	Implementation of this screen	1574:1602	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	0	35	theme	mechanistic	15:25	arg1	analysis					27:34	Structural and mechanistic analysis	0:34	Structural and mechanistic analysis of a β-glycoside phosphorylase	0:65	Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.					
29317495	5	36	theme	ping-pong	996:1004	arg1	mechanism					1006:1014	a double displacement ping-pong mechanism	974:1014	a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate	974:1064	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	1	37	theme	Glycoside	114:122	arg1	phosphorylases					124:137	Glycoside phosphorylases	114:137	Glycoside phosphorylases	114:137	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	3	38	theme	activated	513:521	arg1	β-d-glucoside					551:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside	509:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc)	509:572	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	3	38	theme	activated	513:521	arg1	DNPGlc					566:571	DNPGlc	566:571	DNPGlc	566:571	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	9	39	theme	new	1627:1629	arg1	class					1655:1659	a new glycoside phosphorylase class	1625:1659	a new glycoside phosphorylase class	1625:1659	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	5	40	theme	Kinetic	904:910	arg1	analysis					928:935	Kinetic and mechanistic analysis	904:935	Kinetic and mechanistic analysis of the gene product, BglP,	904:962	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	9	41	theme	enzyme	1716:1721	arg1	specificity					1723:1733	enzyme specificity	1716:1733	enzyme specificity	1716:1733	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	3	42	used	used	651:654	arg2	we					453:454	we	453:454	we	453:454	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	4	43	theme	2,4-dinitrophenyl	745:761	arg1	release					734:740	release	734:740	release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate	734:802	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	6	44	theme	crystallographic	1073:1088	arg1	analysis					1090:1097	X-ray crystallographic analysis	1067:1097	X-ray crystallographic analysis	1067:1097	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	8	45	theme	screening	1401:1409	arg1	approach					1411:1418	a high-throughput screening approach	1383:1418	a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library	1383:1571	In summary, we present a high-throughput screening approach for identifying β-glycoside phosphorylases, which was robust, simple to implement, and useful in identifying active clones within a metagenomics library.					
29317495	4	46	theme	initial	709:715	arg1	screen					717:722	The initial screen	705:722	The initial screen	705:722	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	0	47	theme	phosphorylase	53:65	arg1	analysis					27:34	Structural and mechanistic analysis	0:34	Structural and mechanistic analysis of a β-glycoside phosphorylase	0:65	Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.					
29317495	5	48	theme	gene	944:947	arg1	BglP					958:961	BglP	958:961	BglP	958:961	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	5	48	theme	gene	944:947	arg1	product					949:955	the gene product	940:955	the gene product	940:955	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	6	49	theme	phosphate-binding	1126:1142	arg1	mode					1144:1147	the phosphate-binding mode	1122:1147	the phosphate-binding mode	1122:1147	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	4	50	theme	GH3	892:894	arg1	family					896:901	the CAZy GH3 family	883:901	the CAZy GH3 family	883:901	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	9	51	theme	biotechnological	1790:1805	arg1	applications					1807:1818	biotechnological applications	1790:1818	biotechnological applications	1790:1818	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	0	52	theme	β-glycoside	41:51	arg1	phosphorylase					53:65	a β-glycoside phosphorylase	39:65	a β-glycoside phosphorylase	39:65	Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.					
29317495	7	53	from	glucoside	1324:1332	arg1	specificity					1307:1317	the substrate specificity	1293:1317	the substrate specificity from glucoside to N-acetylglucosaminide	1293:1357	Substituting this glutamine for a serine swapped the substrate specificity from glucoside to N-acetylglucosaminide.					
29317495	4	54	theme	CAZy	887:890	arg1	family					896:901	the CAZy GH3 family	883:901	the CAZy GH3 family	883:901	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	6	55	theme	substrate	1221:1229	arg1	recognition					1231:1241	substrate recognition	1221:1241	substrate recognition	1221:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	4	56	theme	β-glycoside	852:862	arg1	phosphorylase					864:876	a retaining β-glycoside phosphorylase	840:876	a retaining β-glycoside phosphorylase from the CAZy GH3 family	840:901	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	4	57	theme	retaining	842:850	arg1	phosphorylase					864:876	a retaining β-glycoside phosphorylase	840:876	a retaining β-glycoside phosphorylase from the CAZy GH3 family	840:901	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	3	58	theme	metagenomic	684:694	arg1	library					696:702	a large insert metagenomic library	669:702	a large insert metagenomic library	669:702	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	1	59	theme	considerable	144:155	arg1	catalysts					170:178	catalysts	170:178	catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials	170:290	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	1	59	theme	considerable	144:155	arg1	potential					157:165	considerable potential	144:165	considerable potential	144:165	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	6	60	theme	important	1207:1215	arg1	site					1202:1205	the active site	1191:1205	the active site important for substrate recognition	1191:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	1	61	theme	functional	241:250	arg1	foods					252:256	functional foods	241:256	functional foods	241:256	Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials.					
29317495	9	62	theme	devising	1686:1693	arg1	ways					1702:1705	devising simple ways	1686:1705	devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications	1686:1818	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	2	63	theme	phosphorylases	350:363	arg1	small					379:383	small	379:383	small	379:383	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	2	63	theme	phosphorylases	350:363	arg1	diversity					316:324	the substrate diversity	302:324	the substrate diversity of currently identified phosphorylases	302:363	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	3	64	theme	substrate	523:531	arg1	β-d-glucoside					551:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside	509:563	the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc)	509:572	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	3	64	theme	substrate	523:531	arg1	DNPGlc					566:571	DNPGlc	566:571	DNPGlc	566:571	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	5	65	theme	product	949:955	arg1	analysis					928:935	Kinetic and mechanistic analysis	904:935	Kinetic and mechanistic analysis of the gene product, BglP,	904:962	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	3	66	theme	large	671:675	arg1	library					696:702	a large insert metagenomic library	669:702	a large insert metagenomic library	669:702	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	9	67	theme	class	1655:1659	arg1	discovery					1612:1620	discovery	1612:1620	discovery of a new glycoside phosphorylase class	1612:1659	Implementation of this screen enabled discovery of a new glycoside phosphorylase class and has paved the way to devising simple ways in which enzyme specificity can be encoded and swapped, which has implications for biotechnological applications.					
29317495	2	68	theme	practical	401:409	arg1	applications					411:422	their practical applications	395:422	their practical applications	395:422	However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications.					
29317495	6	69	theme	glutamine	1170:1178	arg1	residue					1180:1186	a key glutamine residue	1164:1186	a key glutamine residue in the active site important for substrate recognition	1164:1241	X-ray crystallographic analysis provided insights into the phosphate-binding mode and identified a key glutamine residue in the active site important for substrate recognition.					
29317495	5	70	theme	covalent	1028:1035	arg1	intermediate					1053:1064	a covalent glycosyl-enzyme intermediate	1026:1064	a covalent glycosyl-enzyme intermediate	1026:1064	Kinetic and mechanistic analysis of the gene product, BglP, confirmed a double displacement ping-pong mechanism involving a covalent glycosyl-enzyme intermediate.					
29317495	3	71	theme	insert	677:682	arg1	library					696:702	a large insert metagenomic library	669:702	a large insert metagenomic library	669:702	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	3	72	theme	inorganic	578:586	arg1	phosphate					588:596	inorganic phosphate	578:596	inorganic phosphate for identifying glycoside phosphorylase activity	578:645	To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-d-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library.					
29317495	4	73	from	DNPGlc	768:773	arg1	presence					782:789	the presence	778:789	the presence of phosphate	778:802	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
29317495	4	73	from	DNPGlc	768:773	arg1	release					734:740	release	734:740	release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate	734:802	The initial screen, based on release of 2,4-dinitrophenyl from DNPGlc in the presence of phosphate, identified the gene bglP, encoding a retaining β-glycoside phosphorylase from the CAZy GH3 family.					
31264852	1	0	theme	hydroperoxides	337:350	arg1	set					315:317	a diverse set	305:317	a diverse set of cell-signaling hydroperoxides	305:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	1	0	theme	hydroperoxides	337:350	arg1	hydroperoxides					337:350	cell-signaling hydroperoxides	322:350	cell-signaling hydroperoxides	322:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	4	1	theme	important	703:711	arg1	target					713:718	an important target	700:718	an important target for the devastating rice blast disease	700:757	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	1	2	from	fungi	184:188	arg1	Lipoxygenases					154:166	Lipoxygenases	154:166	Lipoxygenases from pathogenic fungi	154:188	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	0	3	dep	Impact	120:125	arg1	Magnaporthe					100:110	Magnaporthe	100:110	Magnaporthe	100:110	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	0	3	dep	Impact	120:125	arg1	Characterization					8:23	Kinetic Characterization	0:23	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus	0:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	1	4	theme	C-H	251:253	arg1	activation					255:264	C-H activation	251:264	C-H activation of polyunsaturated fatty acids	251:295	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	7	5	theme	ground	1420:1425	arg1	state					1427:1431	the ground state	1416:1431	the ground state	1416:1431	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	8	6	theme	lipoxygenase	1530:1541	arg1	activation					1547:1556	lipoxygenase C-H activation	1530:1556	lipoxygenase C-H activation	1530:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	6	7	theme	disproportionate	1075:1090	arg1	increase					1092:1099	a disproportionate increase	1073:1099	a disproportionate increase in the activation energy for deuterium transfer	1073:1147	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	8	theme	deuterium	1001:1009	arg1	transfer					1011:1018	deuterium transfer	1001:1018	deuterium transfer	1001:1018	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	7	9	theme	positioning	1333:1343	arg1	impairment					1309:1318	an impairment	1306:1318	an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state	1306:1431	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	8	10	theme	C-H	1543:1545	arg1	activation					1547:1556	lipoxygenase C-H activation	1530:1556	lipoxygenase C-H activation	1530:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	8	11	theme	inhibitor	1600:1608	arg1	design					1610:1615	MoLOX inhibitor design	1594:1615	MoLOX inhibitor design	1594:1615	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	6	12	theme	kinetic	1031:1037	arg1	reporter					1039:1046	a kinetic reporter	1029:1046	a kinetic reporter of tunneling efficiency	1029:1070	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	12	theme	kinetic	1031:1037	arg1	barrier					976:982	the differential enthalpic barrier	949:982	the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa,	949:1024	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	0	13	theme	Pathogenic	82:91	arg1	Fungus					93:98	the Pathogenic Fungus	78:98	the Pathogenic Fungus	78:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	3	14	theme	enzymes	565:571	arg1	function					547:554	function	547:554	function	547:554	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	3	14	theme	enzymes	565:571	arg1	structure					533:541	structure	533:541	structure	533:541	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	0	15	dep	Magnaporthe	100:110	arg1	oryzae					112:117	Magnaporthe oryzae	100:117	Magnaporthe oryzae	100:117	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	1	16	theme	polyunsaturated	269:283	arg1	acids					291:295	polyunsaturated fatty acids	269:295	polyunsaturated fatty acids	269:295	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	6	17	theme	deuterium	1130:1138	arg1	transfer					1140:1147	deuterium transfer	1130:1147	deuterium transfer	1130:1147	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	8	18	contain	have	1562:1565	arg2	implications					1577:1588	important implications	1567:1588	important implications for MoLOX inhibitor design	1567:1615	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	8	18	contain	have	1562:1565	arg1	results					1440:1446	These results	1434:1446	These results	1434:1446	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	1	19	theme	pathogenic	173:182	arg1	fungi					184:188	pathogenic fungi	173:188	pathogenic fungi	173:188	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	2	20	theme	N-linked	477:484	arg1	glycans					486:492	N-linked glycans	477:492	N-linked glycans	477:492	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	4	21	theme	exemplary	602:610	arg1	system					612:617	One exemplary system	598:617	One exemplary system	598:617	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	3	22	from	impact	499:504	arg1	function					547:554	function	547:554	function	547:554	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	3	22	from	impact	499:504	arg1	structure					533:541	structure	533:541	structure	533:541	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	8	23	theme	important	1567:1575	arg1	implications					1577:1588	important implications	1567:1588	important implications for MoLOX inhibitor design	1567:1615	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	6	24	theme	differential	953:964	arg1	reporter					1039:1046	a kinetic reporter	1029:1046	a kinetic reporter of tunneling efficiency	1029:1070	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	24	theme	differential	953:964	arg1	barrier					976:982	the differential enthalpic barrier	949:982	the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa,	949:1024	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	2	25	link	N-linked	477:484	arg1	glycans					486:492	N-linked glycans	477:492	N-linked glycans	477:492	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	4	26	theme	blast	745:749	arg1	disease					751:757	the devastating rice blast disease	724:757	the devastating rice blast disease	724:757	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	4	27	from	oryzae	672:677	arg1	MoLOX					622:626	MoLOX	622:626	MoLOX	622:626	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	4	27	from	oryzae	672:677	arg1	lipoxygenase					631:642	a lipoxygenase	629:642	a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease	629:757	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	6	28	theme	efficiency	1061:1070	arg1	reporter					1039:1046	a kinetic reporter	1029:1046	a kinetic reporter of tunneling efficiency	1029:1070	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	28	theme	efficiency	1061:1070	arg1	barrier					976:982	the differential enthalpic barrier	949:982	the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa,	949:1024	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	0	29	theme	Kinetic	0:6	arg1	Characterization					8:23	Kinetic Characterization	0:23	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus	0:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	1	30	theme	fatty	285:289	arg1	acids					291:295	polyunsaturated fatty acids	269:295	polyunsaturated fatty acids	269:295	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	5	31	theme	acid	862:865	arg1	cleavage					827:834	C-H cleavage	823:834	C-H cleavage of the substrate linoleic acid by MoLOX	823:874	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	4	32	theme	rice	740:743	arg1	disease					751:757	the devastating rice blast disease	724:757	the devastating rice blast disease	724:757	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	6	33	theme	tunneling	1051:1059	arg1	efficiency					1061:1070	tunneling efficiency	1051:1070	tunneling efficiency	1051:1070	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	5	34	theme	C-H	823:825	arg1	cleavage					827:834	C-H cleavage	823:834	C-H cleavage of the substrate linoleic acid by MoLOX	823:874	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	1	35	theme	acids	291:295	arg1	activation					255:264	C-H activation	251:264	C-H activation of polyunsaturated fatty acids	251:295	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	7	36	with	consistent	1290:1299	arg1	impairment					1309:1318	an impairment	1306:1318	an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state	1306:1431	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	3	37	dep	structure	533:541	arg1	the					529:531	the	529:531	the	529:531	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	8	38	theme	glycosylation	1513:1525	arg1	consequences					1488:1499	the functional consequences	1473:1499	the functional consequences of N-linked glycosylation on lipoxygenase C-H activation	1473:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	2	39	theme	fungal	443:448	arg1	enzymes					450:456	these fungal enzymes	437:456	these fungal enzymes	437:456	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	6	40	theme	activation	1108:1117	arg1	energy					1119:1124	the activation energy	1104:1124	the activation energy for deuterium transfer	1104:1147	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	41	theme	enthalpic	966:974	arg1	reporter					1039:1046	a kinetic reporter	1029:1046	a kinetic reporter of tunneling efficiency	1029:1070	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	6	41	theme	enthalpic	966:974	arg1	barrier					976:982	the differential enthalpic barrier	949:982	the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa,	949:1024	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	8	42	theme	new	1456:1458	arg1	insight					1460:1466	new insight	1456:1466	new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation	1456:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	8	43	theme	MoLOX	1594:1598	arg1	design					1610:1615	MoLOX inhibitor design	1594:1615	MoLOX inhibitor design	1594:1615	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	1	44	theme	lipoxygenase	204:215	arg1	family					217:222	the lipoxygenase family	200:222	the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides	200:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	0	45	theme	Activation	36:45	arg1	Step					47:50	the C-H Activation Step	28:50	the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus	28:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	6	46	link	N-linked	1227:1234	arg1	carbohydrates					1236:1248	N-linked carbohydrates	1227:1248	N-linked carbohydrates from the protein	1227:1265	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	0	47	theme	N-Linked	130:137	arg1	Glycosylation					139:151	N-Linked Glycosylation	130:151	N-Linked Glycosylation	130:151	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	5	48	theme	substrate	843:851	arg1	acid					862:865	the substrate linoleic acid	839:865	the substrate linoleic acid	839:865	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	7	49	theme	tunneling	1390:1398	arg1	state					1406:1410	the tunneling ready state	1386:1410	the tunneling ready state	1386:1410	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	7	50	theme	substrate	1323:1331	arg1	positioning					1333:1343	substrate positioning	1323:1343	substrate positioning	1323:1343	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	4	51	theme	Magnaporthe	660:670	arg1	oryzae					672:677	the fungus Magnaporthe oryzae	649:677	the fungus Magnaporthe oryzae	649:677	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	0	52	theme	C-H	32:34	arg1	Step					47:50	the C-H Activation Step	28:50	the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus	28:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	5	53	theme	hydrogen	788:795	arg1	transfer					797:804	hydrogen transfer	788:804	hydrogen transfer	788:804	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	6	54	theme	N-linked	1227:1234	arg1	carbohydrates					1236:1248	N-linked carbohydrates	1227:1248	N-linked carbohydrates from the protein	1227:1265	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	7	55	theme	increased	1273:1281	arg1	ΔEa					1283:1285	This increased ΔEa	1268:1285	This increased ΔEa	1268:1285	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	4	56	theme	fungus	653:658	arg1	oryzae					672:677	the fungus Magnaporthe oryzae	649:677	the fungus Magnaporthe oryzae	649:677	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	1	57	theme	diverse	307:313	arg1	set					315:317	a diverse set	305:317	a diverse set of cell-signaling hydroperoxides	305:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	1	57	theme	diverse	307:313	arg1	hydroperoxides					337:350	cell-signaling hydroperoxides	322:350	cell-signaling hydroperoxides	322:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	6	58	with	treatment	1166:1174	arg1	peptide					1192:1198	a peptide	1190:1198	a peptide	1190:1198	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	8	59	theme	N-linked	1504:1511	arg1	glycosylation					1513:1525	N-linked glycosylation	1504:1525	N-linked glycosylation	1504:1525	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	3	60	theme	N-linked	509:516	arg1	glycans					518:524	N-linked glycans	509:524	N-linked glycans	509:524	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	0	61	theme	Step	47:50	arg1	Characterization					8:23	Kinetic Characterization	0:23	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus	0:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	6	62	theme	MoLOX	1179:1183	arg1	treatment					1166:1174	treatment	1166:1174	treatment of MoLOX with a peptide	1166:1198	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	0	63	theme	Glycosylation	139:151	arg1	Impact					120:125	Impact	120:125	Impact of N-Linked Glycosylation	120:151	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	8	64	theme	functional	1477:1486	arg1	consequences					1488:1499	the functional consequences	1473:1499	the functional consequences of N-linked glycosylation on lipoxygenase C-H activation	1473:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	6	65	from	protein	1259:1265	arg1	carbohydrates					1236:1248	N-linked carbohydrates	1227:1248	N-linked carbohydrates from the protein	1227:1265	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	3	66	theme	glycans	518:524	arg1	impact					499:504	The impact	495:504	The impact of N-linked glycans on the structure and function of these enzymes	495:571	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	3	67	link	N-linked	509:516	arg1	glycans					518:524	N-linked glycans	509:524	N-linked glycans	509:524	The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown.					
31264852	7	68	theme	ready	1400:1404	arg1	state					1406:1410	the tunneling ready state	1386:1410	the tunneling ready state	1386:1410	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	4	69	theme	devastating	728:738	arg1	disease					751:757	the devastating rice blast disease	724:757	the devastating rice blast disease	724:757	One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease.					
31264852	1	70	theme	enzymes	227:233	arg1	family					217:222	the lipoxygenase family	200:222	the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides	200:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	7	71	from	impairment	1309:1318	arg1	complex					1369:1375	the enzyme-substrate complex	1348:1375	the enzyme-substrate complex for both the tunneling ready state and the ground state	1348:1431	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	0	72	from	Fungus	93:98	arg1	Lipoxygenase					60:71	the Lipoxygenase	56:71	the Lipoxygenase from the Pathogenic Fungus	56:98	Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation.					
31264852	6	73	from	increase	1092:1099	arg1	energy					1119:1124	the activation energy	1104:1124	the activation energy for deuterium transfer	1104:1147	Using the differential enthalpic barrier for hydrogen and deuterium transfer, ΔEa, as a kinetic reporter of tunneling efficiency, a disproportionate increase in the activation energy for deuterium transfer is observed upon treatment of MoLOX with a peptide:N-glycosidase that cleaves N-linked carbohydrates from the protein.					
31264852	5	74	theme	tunneling	922:930	arg1	mechanism					932:940	a hydrogen tunneling mechanism	911:940	a hydrogen tunneling mechanism	911:940	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	8	75	from	consequences	1488:1499	arg1	activation					1547:1556	lipoxygenase C-H activation	1530:1556	lipoxygenase C-H activation	1530:1556	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31264852	5	76	theme	hydrogen	913:920	arg1	mechanism					932:940	a hydrogen tunneling mechanism	911:940	a hydrogen tunneling mechanism	911:940	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	5	77	theme	linoleic	853:860	arg1	acid					862:865	the substrate linoleic acid	839:865	the substrate linoleic acid	839:865	Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLOX, is rate-determining and occurs by a hydrogen tunneling mechanism.					
31264852	2	78	theme	lipoxygenase	363:374	arg1	similar					428:434	similar	428:434	similar	428:434	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	2	78	theme	lipoxygenase	363:374	arg1	domains					386:392	the lipoxygenase catalytic domains	359:392	the lipoxygenase catalytic domains	359:392	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	1	79	theme	cell-signaling	322:335	arg1	hydroperoxides					337:350	cell-signaling hydroperoxides	322:350	cell-signaling hydroperoxides	322:350	Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides.					
31264852	7	80	theme	enzyme-substrate	1352:1367	arg1	complex					1369:1375	the enzyme-substrate complex	1348:1375	the enzyme-substrate complex for both the tunneling ready state and the ground state	1348:1431	This increased ΔEa is consistent with an impairment of substrate positioning in the enzyme-substrate complex for both the tunneling ready state and the ground state.					
31264852	2	81	theme	catalytic	376:384	arg1	similar					428:434	similar	428:434	similar	428:434	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	2	81	theme	catalytic	376:384	arg1	domains					386:392	the lipoxygenase catalytic domains	359:392	the lipoxygenase catalytic domains	359:392	While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans.					
31264852	8	82	link	N-linked	1504:1511	arg1	glycosylation					1513:1525	N-linked glycosylation	1504:1525	N-linked glycosylation	1504:1525	These results provide new insight into the functional consequences of N-linked glycosylation on lipoxygenase C-H activation and have important implications for MoLOX inhibitor design.					
31179689	7	0	theme	assignments	1488:1498	arg1	accuracy					1469:1476	the accuracy	1465:1476	the accuracy of glycan assignments	1465:1498	To assess the accuracy of glycan assignments, a distance-based confidence metric was used.					
31179689	9	1	theme	Triple	1712:1717	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	5	2	theme	multiple	1202:1209	arg1	attributes					1218:1227	multiple glycan attributes	1202:1227	multiple glycan attributes	1202:1227	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	0	3	theme	Triple	186:191	arg1	Cancer					209:214	Triple Negative Breast Cancer	186:214	Triple Negative Breast Cancer	186:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	5	4	from	accuracy	1254:1261	arg1	assignments					1287:1297	automated structural assignments	1266:1297	automated structural assignments	1266:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	0	5	theme	Breast	202:207	arg1	Cancer					209:214	Triple Negative Breast Cancer	186:214	Triple Negative Breast Cancer	186:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	9	6	theme	glycosylation	1787:1799	arg1	signatures					1801:1810	potential GSL glycosylation signatures	1773:1810	potential GSL glycosylation signatures characteristic of different TNBC subtypes	1773:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	3	7	theme	hydrophilic	589:599	arg1	HILIC					653:657	HILIC	653:657	HILIC	653:657	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	7	theme	hydrophilic	589:599	arg1	chromatography					637:650	hydrophilic interaction high-performance liquid chromatography	589:650	hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	589:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	6	8	contain	containing	1390:1399	arg1	glycans					1382:1388	GSL glycans	1378:1388	GSL glycans containing GU, mass, and CCS values for each glycan	1378:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	6	8	contain	containing	1390:1399	arg2	mass					1405:1408	mass	1405:1408	mass	1405:1408	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	6	8	contain	containing	1390:1399	arg2	GU					1401:1402	GU	1401:1402	GU	1401:1402	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	6	8	contain	containing	1390:1399	arg2	values					1419:1424	CCS values	1415:1424	CCS values for each glycan	1415:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	3	9	theme	glycan	768:773	arg1	assignments					775:785	automated glycan assignments	758:785	automated glycan assignments	758:785	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	6	10	theme	reference	1356:1364	arg1	database					1366:1373	an experimental reference database	1340:1373	an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan	1340:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	3	11	theme	glycans	575:581	arg1	analyses					541:548	Conventional analyses	528:548	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	528:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	0	12	theme	Automated	112:120	arg1	Identifications					147:161	Automated Glycosphingolipid Glycan Identifications	112:161	Automated Glycosphingolipid Glycan Identifications	112:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	8	13	dep	better	1588:1593	arg1	compared					1595:1602	compared	1595:1602	compared to conventional HILIC-MS approaches (using mass and GU only)	1595:1663	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	1	14	theme	Glycan	217:222	arg1	head-groups					224:234	Glycan head-groups	217:234	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer	217:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	4	15	theme	separation	885:894	arg1	level					876:880	an additional level	862:880	an additional level of separation	862:894	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	0	16	theme	Glycan	140:145	arg1	Identifications					147:161	Automated Glycosphingolipid Glycan Identifications	112:161	Automated Glycosphingolipid Glycan Identifications	112:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	3	17	theme	high-performance	613:628	arg1	HILIC					653:657	HILIC	653:657	HILIC	653:657	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	17	theme	high-performance	613:628	arg1	chromatography					637:650	hydrophilic interaction high-performance liquid chromatography	589:650	hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	589:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	9	18	theme	Breast	1728:1733	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	1	19	theme	cellular	413:420	arg1	states					430:435	cellular disease states	413:435	cellular disease states	413:435	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	2	20	theme	structures	497:506	arg1	analyses					478:485	comprehensive analyses	464:485	comprehensive analyses of glycan structures	464:506	To identify such markers, comprehensive analyses of glycan structures must be undertaken.					
31179689	0	21	theme	Cross	44:48	arg1	Values					58:63	Collision Cross Section Values	34:63	Collision Cross Section Values	34:63	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	5	22	theme	GSL	1112:1114	arg1	glycans					1116:1122	procainamide-labeled GSL glycans	1091:1122	procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments	1091:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	5	23	theme	identification	1170:1183	arg1	strategy					1185:1192	a new, automated glycan identification strategy	1146:1192	a new, automated glycan identification strategy	1146:1192	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	1	24	theme	external	334:341	arg1	stimuli					343:349	external stimuli	334:349	external stimuli	334:349	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	9	25	theme	lines	1754:1758	arg1	study					1699:1703	the study	1695:1703	the study of two Triple Negative Breast Cancer (TNBC) cell lines	1695:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	5	26	theme	structural	1276:1285	arg1	assignments					1287:1297	automated structural assignments	1266:1297	automated structural assignments	1266:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	0	27	theme	Multiattribute	66:79	arg1	Data					81:84	Multiattribute Data	66:84	Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer	66:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	4	28	theme	structures	956:965	arg1	characterization					908:923	the characterization	904:923	the characterization of closely related or isomeric structures	904:965	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	2	29	theme	comprehensive	464:476	arg1	analyses					478:485	comprehensive analyses	464:485	comprehensive analyses of glycan structures	464:506	To identify such markers, comprehensive analyses of glycan structures must be undertaken.					
31179689	9	30	theme	GSL	1783:1785	arg1	signatures					1801:1810	potential GSL glycosylation signatures	1773:1810	potential GSL glycosylation signatures characteristic of different TNBC subtypes	1773:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	0	31	from	Application	171:181	arg1	Identifications					147:161	Automated Glycosphingolipid Glycan Identifications	112:161	Automated Glycosphingolipid Glycan Identifications	112:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	0	31	from	Application	171:181	arg1	Cancer					209:214	Triple Negative Breast Cancer	186:214	Triple Negative Breast Cancer	186:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	4	32	theme	identifiers	1030:1040	arg1	generation					979:988	the generation	975:988	the generation of glycan collision cross section (CCS) identifiers	975:1040	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	1	33	theme	cell	287:290	arg1	bilayer					301:307	the cell membrane bilayer	283:307	the cell membrane bilayer	283:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	4	34	theme	collision	1000:1008	arg1	CCS					1025:1027	CCS	1025:1027	CCS	1025:1027	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	4	34	theme	collision	1000:1008	arg1	section					1016:1022	glycan collision cross section	993:1022	glycan collision cross section (CCS) identifiers	993:1040	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	7	35	used	used	1540:1543	arg2	metric					1529:1534	a distance-based confidence metric	1501:1534	a distance-based confidence metric	1501:1534	To assess the accuracy of glycan assignments, a distance-based confidence metric was used.					
31179689	9	36	theme	different	1830:1838	arg1	subtypes					1845:1852	different TNBC subtypes	1830:1852	different TNBC subtypes	1830:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	4	37	theme	section	1016:1022	arg1	identifiers					1030:1040	glycan collision cross section (CCS) identifiers	993:1040	glycan collision cross section (CCS) identifiers	993:1040	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	3	38	theme	mass	673:676	arg1	spectrometry					678:689	mass spectrometry	673:689	mass spectrometry (MS)	673:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	38	theme	mass	673:676	arg1	MS					692:693	MS	692:693	MS	692:693	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	4	39	theme	ion	841:843	arg1	IM					855:856	IM	855:856	IM	855:856	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	4	39	theme	ion	841:843	arg1	mobility					845:852	ion mobility	841:852	ion mobility (IM)	841:857	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	5	40	theme	new	1148:1150	arg1	strategy					1185:1192	a new, automated glycan identification strategy	1146:1192	a new, automated glycan identification strategy	1146:1192	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	7	41	theme	distance-based	1503:1516	arg1	metric					1529:1534	a distance-based confidence metric	1501:1534	a distance-based confidence metric	1501:1534	To assess the accuracy of glycan assignments, a distance-based confidence metric was used.					
31179689	3	42	theme	relative	705:712	arg1	quantitation					714:725	relative quantitation	705:725	relative quantitation	705:725	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	8	43	theme	conventional	1607:1618	arg1	approaches					1629:1638	conventional HILIC-MS approaches	1607:1638	conventional HILIC-MS approaches (using mass and GU only)	1607:1663	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	0	44	theme	Collision	34:42	arg1	Values					58:63	Collision Cross Section Values	34:63	Collision Cross Section Values	34:63	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	7	45	theme	glycan	1481:1486	arg1	assignments					1488:1498	glycan assignments	1481:1498	glycan assignments	1481:1498	To assess the accuracy of glycan assignments, a distance-based confidence metric was used.					
31179689	0	46	dep	Combining	0:8	arg1	Data					81:84	Multiattribute Data	66:84	Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer	66:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	0	47	from	Accuracy	100:107	arg1	Identifications					147:161	Automated Glycosphingolipid Glycan Identifications	112:161	Automated Glycosphingolipid Glycan Identifications	112:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	0	47	from	Accuracy	100:107	arg1	Cancer					209:214	Triple Negative Breast Cancer	186:214	Triple Negative Breast Cancer	186:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	0	48	theme	Negative	193:200	arg1	Cancer					209:214	Triple Negative Breast Cancer	186:214	Triple Negative Breast Cancer	186:214	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	5	49	theme	glycan	1211:1216	arg1	attributes					1218:1227	multiple glycan attributes	1202:1227	multiple glycan attributes	1202:1227	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	4	50	theme	additional	865:874	arg1	level					876:880	an additional level	862:880	an additional level of separation	862:894	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	3	51	theme	automated	758:766	arg1	assignments					775:785	automated glycan assignments	758:785	automated glycan assignments	758:785	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	52	theme	Conventional	528:539	arg1	analyses					541:548	Conventional analyses	528:548	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	528:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	2	53	theme	such	450:453	arg1	markers					455:461	such markers	450:461	such markers	450:461	To identify such markers, comprehensive analyses of glycan structures must be undertaken.					
31179689	9	54	theme	TNBC	1840:1843	arg1	subtypes					1845:1852	different TNBC subtypes	1830:1852	different TNBC subtypes	1830:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	4	55	theme	mobility	845:852	arg1	use					834:836	The use	830:836	The use of ion mobility (IM) as an additional level of separation	830:894	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	3	56	theme	glucose	793:799	arg1	GU					807:808	GU	807:808	GU	807:808	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	56	theme	glucose	793:799	arg1	unit					801:804	glucose unit	793:804	glucose unit (GU)	793:809	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	57	theme	labeled	567:573	arg1	glycans					575:581	fluorescently labeled glycans	553:581	fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	553:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	6	58	theme	experimental	1343:1354	arg1	database					1366:1373	an experimental reference database	1340:1373	an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan	1340:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	1	59	theme	potential	376:384	arg1	markers					386:392	potential markers	376:392	potential markers	376:392	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	3	60	theme	liquid	630:635	arg1	HILIC					653:657	HILIC	653:657	HILIC	653:657	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	60	theme	liquid	630:635	arg1	chromatography					637:650	hydrophilic interaction high-performance liquid chromatography	589:650	hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	589:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	9	61	theme	TNBC	1743:1746	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	3	62	theme	interaction	601:611	arg1	HILIC					653:657	HILIC	653:657	HILIC	653:657	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	62	theme	interaction	601:611	arg1	chromatography					637:650	hydrophilic interaction high-performance liquid chromatography	589:650	hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	589:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	9	63	theme	Negative	1719:1726	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	9	64	theme	Cancer	1735:1740	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	0	65	theme	Section	50:56	arg1	Values					58:63	Collision Cross Section Values	34:63	Collision Cross Section Values	34:63	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	1	66	theme	disease	422:428	arg1	states					430:435	cellular disease states	413:435	cellular disease states	413:435	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	2	67	theme	glycan	490:495	arg1	structures					497:506	glycan structures	490:506	glycan structures	490:506	To identify such markers, comprehensive analyses of glycan structures must be undertaken.					
31179689	6	68	theme	glycans	1382:1388	arg1	database					1366:1373	an experimental reference database	1340:1373	an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan	1340:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	5	69	theme	glycans	1116:1122	arg1	analysis					1079:1086	the analysis	1075:1086	the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments	1075:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	9	70	theme	cell	1749:1752	arg1	lines					1754:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	two Triple Negative Breast Cancer (TNBC) cell lines	1708:1758	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	3	71	contain	has	731:733	arg1	analyses					541:548	Conventional analyses	528:548	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS)	528:694	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	3	71	contain	has	731:733	arg2	ability					739:745	the ability to perform automated glycan assignments using glucose unit (GU) and mass matching	735:827	the ability to perform automated glycan assignments using glucose unit (GU) and mass matching	735:827	Conventional analyses of fluorescently labeled glycans using hydrophilic interaction high-performance liquid chromatography (HILIC) coupled with mass spectrometry (MS) provides relative quantitation and has the ability to perform automated glycan assignments using glucose unit (GU) and mass matching.					
31179689	5	72	theme	automated	1266:1274	arg1	assignments					1287:1297	automated structural assignments	1266:1297	automated structural assignments	1266:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	4	73	theme	related	936:942	arg1	structures					956:965	closely related or isomeric structures	928:965	closely related or isomeric structures	928:965	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	5	74	dep	new	1148:1150	arg1	automated					1153:1161	automated	1153:1161	automated	1153:1161	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	6	75	theme	GSL	1378:1380	arg1	glycans					1382:1388	GSL glycans	1378:1388	GSL glycans containing GU, mass, and CCS values for each glycan	1378:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	8	76	dep	approaches	1629:1638	arg1	using					1641:1645	using	1641:1645	using mass and GU only	1641:1662	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	4	77	theme	isomeric	947:954	arg1	structures					956:965	closely related or isomeric structures	928:965	closely related or isomeric structures	928:965	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	0	78	theme	Increased	90:98	arg1	Accuracy					100:107	Increased Accuracy	90:107	Increased Accuracy in Automated Glycosphingolipid Glycan Identifications	90:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	0	79	theme	Glucose	10:16	arg1	Units					18:22	Glucose Units	10:22	Glucose Units	10:22	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	5	80	theme	procainamide-labeled	1091:1110	arg1	glycans					1116:1122	procainamide-labeled GSL glycans	1091:1122	procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments	1091:1297	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	0	81	theme	Glycosphingolipid	122:138	arg1	Identifications					147:161	Automated Glycosphingolipid Glycan Identifications	112:161	Automated Glycosphingolipid Glycan Identifications	112:161	Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer.					
31179689	8	82	theme	assignment	1550:1559	arg1	accuracy					1561:1568	The assignment accuracy	1546:1568	The assignment accuracy	1546:1568	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	8	82	theme	assignment	1550:1559	arg1	better					1588:1593	better	1588:1593	better	1588:1593	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	5	83	theme	glycan	1163:1168	arg1	strategy					1185:1192	a new, automated glycan identification strategy	1146:1192	a new, automated glycan identification strategy	1146:1192	Here, we present a workflow for the analysis of procainamide-labeled GSL glycans using HILIC-IM-MS and a new, automated glycan identification strategy whereby multiple glycan attributes are combined to increase accuracy in automated structural assignments.					
31179689	1	84	attach	attached	236:243	arg2	head-groups					224:234	Glycan head-groups	217:234	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer	217:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	1	84	attach	attached	236:243	arg1	glycosphingolipids					248:265	glycosphingolipids	248:265	glycosphingolipids (GSLs) found in the cell membrane bilayer	248:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	1	84	attach	attached	236:243	arg1	GSLs					268:271	GSLs	268:271	GSLs	268:271	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	9	85	theme	subtypes	1845:1852	arg1	characteristic					1812:1825	characteristic	1812:1825	characteristic	1812:1825	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	9	86	theme	characteristic	1812:1825	arg1	signatures					1801:1810	potential GSL glycosylation signatures	1773:1810	potential GSL glycosylation signatures characteristic of different TNBC subtypes	1773:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	1	87	theme	membrane	292:299	arg1	bilayer					301:307	the cell membrane bilayer	283:307	the cell membrane bilayer	283:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	4	88	theme	glycan	993:998	arg1	CCS					1025:1027	CCS	1025:1027	CCS	1025:1027	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	4	88	theme	glycan	993:998	arg1	section					1016:1022	glycan collision cross section	993:1022	glycan collision cross section (CCS) identifiers	993:1040	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	9	89	theme	potential	1773:1781	arg1	signatures					1801:1810	potential GSL glycosylation signatures	1773:1810	potential GSL glycosylation signatures characteristic of different TNBC subtypes	1773:1852	This workflow was applied to the study of two Triple Negative Breast Cancer (TNBC) cell lines and revealed potential GSL glycosylation signatures characteristic of different TNBC subtypes.					
31179689	6	90	theme	CCS	1415:1417	arg1	values					1419:1424	CCS values	1415:1424	CCS values for each glycan	1415:1440	For glycan matching and identification, an experimental reference database of GSL glycans containing GU, mass, and CCS values for each glycan was created.					
31179689	4	91	theme	cross	1010:1014	arg1	CCS					1025:1027	CCS	1025:1027	CCS	1025:1027	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	4	91	theme	cross	1010:1014	arg1	section					1016:1022	glycan collision cross section	993:1022	glycan collision cross section (CCS) identifiers	993:1040	The use of ion mobility (IM) as an additional level of separation can aid the characterization of closely related or isomeric structures through the generation of glycan collision cross section (CCS) identifiers.					
31179689	7	92	theme	confidence	1518:1527	arg1	metric					1529:1534	a distance-based confidence metric	1501:1534	a distance-based confidence metric	1501:1534	To assess the accuracy of glycan assignments, a distance-based confidence metric was used.					
31179689	8	93	theme	HILIC-MS	1620:1627	arg1	approaches					1629:1638	conventional HILIC-MS approaches	1607:1638	conventional HILIC-MS approaches (using mass and GU only)	1607:1663	The assignment accuracy was significantly better compared to conventional HILIC-MS approaches (using mass and GU only).					
31179689	1	94	located	found	274:278	arg2	GSLs					268:271	GSLs	268:271	GSLs	268:271	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	1	94	located	found	274:278	arg2	glycosphingolipids					248:265	glycosphingolipids	248:265	glycosphingolipids (GSLs) found in the cell membrane bilayer	248:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31179689	1	94	located	found	274:278	arg1	bilayer					301:307	the cell membrane bilayer	283:307	the cell membrane bilayer	283:307	Glycan head-groups attached to glycosphingolipids (GSLs) found in the cell membrane bilayer can alter in response to external stimuli and disease, making them potential markers and/or targets for cellular disease states.					
31790908	0	0	from	fold	11:14	arg1	structure					43:51	the structure	39:51	the structure of ICChI, a protein with chitinase and lysozyme activity	39:108	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	8	1	theme	consensus	972:980	arg1	sites					982:986	the consensus sites	968:986	the consensus sites for N-glycosylation viz.	968:1011	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	7	2	theme	triosephosphate	920:934	arg1	isomerase					936:944	a (α/β)8 triosephosphate isomerase	911:944	a (α/β)8 triosephosphate isomerase barrel fold	911:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	3	3	theme	crystal	431:437	arg1	resolution					449:458	a single crystal to 1.42 Å resolution	422:458	a single crystal to 1.42 Å resolution	422:458	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	1	4	theme	carnea	199:204	arg1	latex					173:177	the latex	169:177	the latex of the weed Ipomoea carnea	169:204	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	0	5	theme	ICChI	56:60	arg1	structure					43:51	the structure	39:51	the structure of ICChI, a protein with chitinase and lysozyme activity	39:108	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	5	6	from	model	678:682	arg1	sequence					735:742	the N-terminal sequence	720:742	the N-terminal sequence of ICChI	720:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	4	7	theme	space	509:513	arg1	P43212					521:526	the primitive tetragonal space group P43212	484:526	the primitive tetragonal space group P43212	484:526	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	5	8	theme	mixed	672:676	arg1	model					678:682	a mixed model	670:682	a mixed model of three homologous structures from the N-terminal sequence of ICChI	670:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	5	9	from	sequence	735:742	arg1	structures					704:713	three homologous structures	687:713	three homologous structures from the N-terminal sequence of ICChI	687:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	5	9	from	sequence	735:742	arg1	model					678:682	a mixed model	670:682	a mixed model of three homologous structures from the N-terminal sequence of ICChI	670:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	6	10	theme	refined	758:764	arg1	model					766:770	The refined model	754:770	The refined model	754:770	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	7	11	theme	globular	890:897	arg1	domain					899:904	a single globular domain	881:904	a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold	881:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	1	12	gly	glycosylated	134:145	arg1	ICChI					115:119	The ICChI	111:119	The ICChI	111:119	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	1	12	gly	glycosylated	134:145	arg1	protein					147:153	a 35-kDa, glycosylated protein	124:153	protein	147:153	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	10	13	theme	family	1259:1264	arg1	chitinases					1266:1275	GH18 family chitinases	1254:1275	GH18 family chitinases	1254:1275	The crystal structure may provide fundamental information of GH18 family chitinases.					
31790908	2	14	theme	lysozyme	233:240	arg1	activity					242:249	chitinase and lysozyme activity	219:249	chitinase and lysozyme activity	219:249	It displays chitinase and lysozyme activity, which could be important for the defense against pathogenic fungi, insects and bacteria.					
31790908	10	15	theme	GH18	1254:1257	arg1	chitinases					1266:1275	GH18 family chitinases	1254:1275	GH18 family chitinases	1254:1275	The crystal structure may provide fundamental information of GH18 family chitinases.					
31790908	1	16	theme	35-kDa	126:131	arg1	ICChI					115:119	The ICChI	111:119	The ICChI	111:119	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	1	16	theme	35-kDa	126:131	arg1	protein					147:153	a 35-kDa, glycosylated protein	124:153	protein	147:153	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	5	17	theme	ICChI	747:751	arg1	sequence					735:742	the N-terminal sequence	720:742	the N-terminal sequence of ICChI	720:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	4	18	theme	group	515:519	arg1	P43212					521:526	the primitive tetragonal space group P43212	484:526	the primitive tetragonal space group P43212	484:526	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	6	19	theme	acid	794:797	arg1	residues					799:806	272 amino acid residues	784:806	272 amino acid residues	784:806	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	2	20	theme	chitinase	219:227	arg1	activity					242:249	chitinase and lysozyme activity	219:249	chitinase and lysozyme activity	219:249	It displays chitinase and lysozyme activity, which could be important for the defense against pathogenic fungi, insects and bacteria.					
31790908	7	21	theme	barrel	946:951	arg1	fold					953:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	1	22	theme	glycosylated	134:145	arg1	ICChI					115:119	The ICChI	111:119	The ICChI	111:119	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	1	22	theme	glycosylated	134:145	arg1	protein					147:153	a 35-kDa, glycosylated protein	124:153	protein	147:153	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	8	23	theme	N-glycosylation	992:1006	arg1	viz.					1008:1011	N-glycosylation viz.	992:1011	N-glycosylation viz.	992:1011	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	0	24	with	protein	65:71	arg1	activity					101:108	lysozyme activity	92:108	lysozyme activity	92:108	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	0	24	with	protein	65:71	arg1	chitinase					78:86	chitinase	78:86	chitinase	78:86	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	8	25	theme	carbohydrate	1051:1062	arg1	xylose					1121:1126	xylose	1121:1126	xylose	1121:1126	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	25	theme	carbohydrate	1051:1062	arg1	mannose					1100:1106	mannose	1100:1106	mannose	1100:1106	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	25	theme	carbohydrate	1051:1062	arg1	fucose					1109:1114	fucose	1109:1114	fucose	1109:1114	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	25	theme	carbohydrate	1051:1062	arg1	moieties					1064:1071	carbohydrate moieties N-Acetylglucosamine (NAG)	1051:1097	carbohydrate moieties N-Acetylglucosamine (NAG)	1051:1097	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	0	26	theme	lysozyme	92:99	arg1	activity					101:108	lysozyme activity	92:108	lysozyme activity	92:108	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	6	27	theme	%	834:834	arg1	Rfactor					818:824	a Rfactor	816:824	a Rfactor of 18.93% and Rfree of 22.42%	816:854	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	4	28	theme	tetragonal	498:507	arg1	P43212					521:526	the primitive tetragonal space group P43212	484:526	the primitive tetragonal space group P43212	484:526	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	3	29	dep	resolution	449:458	arg1	to					439:440	to	439:440	to	439:440	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	6	30	theme	Rfree	840:844	arg1	Rfactor					818:824	a Rfactor	816:824	a Rfactor of 18.93% and Rfree of 22.42%	816:854	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	5	31	theme	N-terminal	724:733	arg1	sequence					735:742	the N-terminal sequence	720:742	the N-terminal sequence of ICChI	720:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	4	32	theme	primitive	488:496	arg1	P43212					521:526	the primitive tetragonal space group P43212	484:526	the primitive tetragonal space group P43212	484:526	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	3	33	theme	diffraction	382:392	arg1	set					399:401	a diffraction data set	380:401	a diffraction data set	380:401	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	0	34	theme	glycan	20:25	arg1	moieties					27:34	glycan moieties	20:34	glycan moieties	20:34	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	8	35	theme	sites	982:986	arg1	sites					982:986	the consensus sites	968:986	the consensus sites for N-glycosylation viz.	968:1011	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	35	theme	sites	982:986	arg1	Three					959:963	Three	959:963	Three	959:963	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	35	theme	sites	982:986	arg1	Asn172					1021:1026	Asn172	1021:1026	Asn172	1021:1026	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	35	theme	sites	982:986	arg1	Asn194					1033:1038	Asn194	1033:1038	Asn194	1033:1038	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	35	theme	sites	982:986	arg1	Asn45					1014:1018	Asn45	1014:1018	Asn45	1014:1018	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	7	36	with	domain	899:904	arg1	fold					953:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	7	37	theme	α/β	914:916	arg1	isomerase					936:944	a (α/β)8 triosephosphate isomerase	911:944	a (α/β)8 triosephosphate isomerase barrel fold	911:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	3	38	theme	data	394:397	arg1	set					399:401	a diffraction data set	380:401	a diffraction data set	380:401	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	7	39	theme	isomerase	936:944	arg1	fold					953:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	a (α/β)8 triosephosphate isomerase barrel fold	911:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	0	40	from	structure	43:51	arg1	fold					11:14	fold	11:14	fold	11:14	TIM barrel fold and glycan moieties in the structure of ICChI, a protein with chitinase and lysozyme activity.					
31790908	3	41	theme	ICChI	345:349	arg1	enzyme					351:356	The ICChI enzyme	341:356	The ICChI enzyme	341:356	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	6	42	theme	%	854:854	arg1	Rfree					840:844	Rfree	840:844	Rfree	840:844	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	6	42	theme	%	854:854	arg1	%					854:854	22.42%	849:854	22.42%	849:854	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	6	42	theme	%	854:854	arg1	%					834:834	18.93%	829:834	18.93%	829:834	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	5	43	theme	replacement	645:655	arg1	method					657:662	molecular replacement method	635:662	molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI	635:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	6	44	theme	amino	788:792	arg1	residues					799:806	272 amino acid residues	784:806	272 amino acid residues	784:806	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	4	45	dep	parameters	544:553	arg1	parameters					544:553	unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°	534:600	unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°	534:600	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	4	45	dep	parameters	544:553	arg1	c = 172.0 Å					569:579	c = 172.0 Å	569:579	c = 172.0 Å	569:579	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	4	45	dep	parameters	544:553	arg1	α = β = γ = 90°					586:600	α = β = γ = 90°	586:600	α = β = γ = 90°	586:600	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	4	45	dep	parameters	544:553	arg1	a = b = 57.9					555:566	a = b = 57.9	555:566	a = b = 57.9	555:566	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	10	46	theme	crystal	1197:1203	arg1	structure					1205:1213	The crystal structure	1193:1213	The crystal structure	1193:1213	The crystal structure may provide fundamental information of GH18 family chitinases.					
31790908	3	47	theme	single	424:429	arg1	resolution					449:458	a single crystal to 1.42 Å resolution	422:458	a single crystal to 1.42 Å resolution	422:458	The ICChI enzyme was crystallized, and a diffraction data set was collected from a single crystal to 1.42 Å resolution.					
31790908	8	48	dep	moieties	1064:1071	arg1	NAG					1094:1096	NAG	1094:1096	NAG	1094:1096	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	8	48	dep	moieties	1064:1071	arg1	N-Acetylglucosamine					1073:1091	N-Acetylglucosamine	1073:1091	carbohydrate moieties N-Acetylglucosamine (NAG)	1051:1097	Three of the consensus sites for N-glycosylation viz., Asn45, Asn172, and Asn194 containing carbohydrate moieties N-Acetylglucosamine (NAG), mannose, fucose, and xylose.					
31790908	1	49	attach	isolated	155:162	arg2	ICChI					115:119	The ICChI	111:119	The ICChI	111:119	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	1	49	attach	isolated	155:162	arg2	protein					147:153	a 35-kDa, glycosylated protein	124:153	protein	147:153	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	1	49	attach	isolated	155:162	arg1	latex					173:177	the latex	169:177	the latex of the weed Ipomoea carnea	169:204	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	10	50	theme	chitinases	1266:1275	arg1	information					1239:1249	fundamental information	1227:1249	fundamental information of GH18 family chitinases	1227:1275	The crystal structure may provide fundamental information of GH18 family chitinases.					
31790908	10	51	theme	fundamental	1227:1237	arg1	information					1239:1249	fundamental information	1227:1249	fundamental information of GH18 family chitinases	1227:1275	The crystal structure may provide fundamental information of GH18 family chitinases.					
31790908	4	52	theme	unit-cell	534:542	arg1	parameters					544:553	unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°	534:600	unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°	534:600	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	4	52	theme	unit-cell	534:542	arg1	c = 172.0 Å					569:579	c = 172.0 Å	569:579	c = 172.0 Å	569:579	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	4	52	theme	unit-cell	534:542	arg1	a = b = 57.9					555:566	a = b = 57.9	555:566	a = b = 57.9	555:566	The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 57.9, c = 172.0 Å, and α = β = γ = 90°.					
31790908	5	53	theme	molecular	635:643	arg1	method					657:662	molecular replacement method	635:662	molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI	635:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	9	54	theme	catalytic	1142:1150	arg1	Asp125					1165:1170	Asp125	1165:1170	Asp125	1165:1170	The putative catalytic residues are Asp125, Glu127, and Tyr184.					
31790908	9	54	theme	catalytic	1142:1150	arg1	residues					1152:1159	The putative catalytic residues	1129:1159	The putative catalytic residues	1129:1159	The putative catalytic residues are Asp125, Glu127, and Tyr184.					
31790908	6	55	contain	has	812:814	arg2	Rfactor					818:824	a Rfactor	816:824	a Rfactor of 18.93% and Rfree of 22.42%	816:854	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	6	55	contain	has	812:814	arg1	model					766:770	The refined model	754:770	The refined model	754:770	The refined model consists of 272 amino acid residues and has a Rfactor of 18.93% and Rfree of 22.42%.					
31790908	5	56	theme	homologous	693:702	arg1	structures					704:713	three homologous structures	687:713	three homologous structures from the N-terminal sequence of ICChI	687:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	2	57	theme	pathogenic	301:310	arg1	fungi					312:316	pathogenic fungi	301:316	pathogenic fungi	301:316	It displays chitinase and lysozyme activity, which could be important for the defense against pathogenic fungi, insects and bacteria.					
31790908	9	58	theme	putative	1133:1140	arg1	Asp125					1165:1170	Asp125	1165:1170	Asp125	1165:1170	The putative catalytic residues are Asp125, Glu127, and Tyr184.					
31790908	9	58	theme	putative	1133:1140	arg1	residues					1152:1159	The putative catalytic residues	1129:1159	The putative catalytic residues	1129:1159	The putative catalytic residues are Asp125, Glu127, and Tyr184.					
31790908	1	59	theme	weed	186:189	arg1	carnea					199:204	the weed Ipomoea carnea	182:204	the weed Ipomoea carnea	182:204	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
31790908	5	60	theme	structures	704:713	arg1	model					678:682	a mixed model	670:682	a mixed model of three homologous structures from the N-terminal sequence of ICChI	670:751	The structure was elucidated by molecular replacement method using a mixed model of three homologous structures from the N-terminal sequence of ICChI.					
31790908	7	61	theme	single	883:888	arg1	domain					899:904	a single globular domain	881:904	a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold	881:956	The protein consists of a single globular domain with a (α/β)8 triosephosphate isomerase barrel fold.					
31790908	1	62	theme	Ipomoea	191:197	arg1	carnea					199:204	the weed Ipomoea carnea	182:204	the weed Ipomoea carnea	182:204	The ICChI is a 35-kDa, glycosylated protein isolated from the latex of the weed Ipomoea carnea.					
30186849	4	0	theme	discovery	946:954	arg1	rate					956:959	a false discovery rate	938:959	a false discovery rate	938:959	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	2	1	gly	glycopeptides	455:467	arg2	glycopeptides					455:467	glycopeptides	455:467	glycopeptides	455:467	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	1	2	theme	spectrometry-based	162:179	arg1	glycoproteomics					181:195	mass spectrometry-based glycoproteomics	157:195	mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	157:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	3	theme	fragmentation	281:293	arg1	microheterogeneity					204:221	microheterogeneity	204:221	microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	204:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	4	4	theme	false	940:944	arg1	rate					956:959	a false discovery rate	938:959	a false discovery rate	938:959	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	4	5	dep	estimated	964:972	arg1	3					935:935	3	935:935	3	935:935	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	3	6	theme	glycopeptide	582:593	arg1	identification					595:608	intact N-linked glycopeptide identification	566:608	intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	566:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	2	7	theme	peptide	388:394	arg1	identification					396:409	peptide identification	388:409	peptide identification	388:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	4	8	theme	open	867:870	arg1	manner					893:898	an open (precursor tolerant) manner	864:898	an open (precursor tolerant) manner	864:898	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	4	9	theme	MS/MS	792:796	arg1	spectra					798:804	MS/MS spectra	792:804	(2) MS/MS spectra of glycopeptides	788:821	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	3	10	theme	search	550:555	arg1	pMatchGlyco					514:524	pMatchGlyco	514:524	pMatchGlyco	514:524	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	10	theme	search	550:555	arg1	tool					557:560	a new spectral library search tool	527:560	a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	527:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	2	11	theme	spectral	323:330	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	11	theme	spectral	323:330	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	12	theme	library	332:338	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	12	theme	library	332:338	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	1	13	theme	peptide	298:304	arg1	bones					306:310	peptide bones	298:310	peptide bones	298:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	14	gly	microheterogeneity	204:221	arg1	complexity					241:250	complexity	241:250	complexity of glycans	241:261	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	14	gly	microheterogeneity	204:221	arg1	fragmentation					281:293	insufficient fragmentation	268:293	insufficient fragmentation of peptide bones	268:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	14	gly	microheterogeneity	204:221	arg1	glycosylation					226:238	glycosylation	226:238	glycosylation	226:238	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	2	15	theme	mass	318:321	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	15	theme	mass	318:321	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	1	16	theme	bones	306:310	arg1	complexity					241:250	complexity	241:250	complexity of glycans	241:261	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	16	theme	bones	306:310	arg1	fragmentation					281:293	insufficient fragmentation	268:293	insufficient fragmentation of peptide bones	268:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	16	theme	bones	306:310	arg1	glycosylation					226:238	glycosylation	226:238	glycosylation	226:238	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	17	gly	glycopeptides	118:130	arg2	glycopeptides					118:130	intact glycopeptides	111:130	intact glycopeptides	111:130	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	5	18	theme	glycoprotein	1156:1167	arg1	set					1174:1176	a complex glycoprotein data set	1146:1176	a complex glycoprotein data set generated from human cancer cell line OVCAR3	1146:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	2	19	theme	Open	313:316	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	19	theme	Open	313:316	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	1	20	theme	due	197:199	arg1	glycoproteomics					181:195	mass spectrometry-based glycoproteomics	157:195	mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	157:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	4	21	gly	glycopeptides	809:821	arg2	glycopeptides					809:821	glycopeptides	809:821	glycopeptides	809:821	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	5	22	theme	set	1174:1176	arg1	sample					1105:1110	mixture sample	1097:1110	mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1097:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	1	23	gly	glycosylation	226:238	arg1	glycans					255:261	glycans	255:261	glycans	255:261	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	23	gly	glycosylation	226:238	arg1	bones					306:310	peptide bones	298:310	peptide bones	298:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	24	theme	Confident	81:89	arg1	task					149:152	a challenging task	135:152	a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	135:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	24	theme	Confident	81:89	arg1	characterization					91:106	Confident characterization	81:106	Confident characterization of intact glycopeptides	81:130	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	3	25	link	N-linked	573:580	arg1	identification					595:608	intact N-linked glycopeptide identification	566:608	intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	566:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	0	26	theme	Glycopeptide	9:20	arg1	Identification					22:35	N-Linked Glycopeptide Identification	0:35	N-Linked Glycopeptide Identification	0:35	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	4	27	from	spectra	842:848	arg1	library					853:859	library	853:859	library	853:859	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	1	28	from	task	149:152	arg1	glycoproteomics					181:195	mass spectrometry-based glycoproteomics	157:195	mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	157:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	3	29	theme	high-energy	616:626	arg1	data					692:695	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	4	30	dep	spectra	798:804	arg1	2					789:789	2	789:789	2	789:789	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	0	31	theme	N-Linked	0:7	arg1	Identification					22:35	N-Linked Glycopeptide Identification	0:35	N-Linked Glycopeptide Identification	0:35	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	4	32	theme	top-scored	978:987	arg1	matches					989:995	top-scored matches	978:995	top-scored matches above a threshold	978:1013	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	5	33	theme	mixture	1097:1103	arg1	sample					1105:1110	mixture sample	1097:1110	mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1097:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	3	34	theme	collisional	628:638	arg1	MS/MS					685:689	MS/MS	685:689	MS/MS	685:689	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	34	theme	collisional	628:638	arg1	spectrometry					671:682	collisional dissociation (HCD) tandem mass spectrometry	628:682	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	4	35	theme	MS/MS	718:722	arg1	spectra					724:730	MS/MS spectra	718:730	(1) MS/MS spectra of deglycopeptides	714:749	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	5	36	theme	human	1193:1197	arg1	line					1211:1214	human cancer cell line OVCAR3	1193:1221	human cancer cell line OVCAR3	1193:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	4	37	theme	glycopeptides	809:821	arg1	spectra					798:804	MS/MS spectra	792:804	(2) MS/MS spectra of glycopeptides	788:821	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	1	38	theme	intact	111:116	arg1	glycopeptides					118:130	intact glycopeptides	111:130	intact glycopeptides	111:130	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	39	theme	glycosylation	226:238	arg1	microheterogeneity					204:221	microheterogeneity	204:221	microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	204:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	4	40	gly	deglycopeptides	735:749	arg2	deglycopeptides					735:749	deglycopeptides	735:749	deglycopeptides	735:749	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	1	41	theme	glycopeptides	118:130	arg1	task					149:152	a challenging task	135:152	a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	135:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	41	theme	glycopeptides	118:130	arg1	characterization					91:106	Confident characterization	81:106	Confident characterization of intact glycopeptides	81:130	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	5	42	theme	cell	1206:1209	arg1	line					1211:1214	human cancer cell line OVCAR3	1193:1221	human cancer cell line OVCAR3	1193:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	2	43	theme	glycopeptides	455:467	arg1	identification					437:450	the identification	433:450	the identification of glycopeptides	433:467	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	0	44	theme	Open	46:49	arg1	Search					73:78	Open Mass Spectral Library Search	46:78	Open Mass Spectral Library Search	46:78	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	5	45	theme	complex	1148:1154	arg1	set					1174:1176	a complex glycoprotein data set	1146:1176	a complex glycoprotein data set generated from human cancer cell line OVCAR3	1146:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	3	46	theme	HCD	654:656	arg1	MS/MS					685:689	MS/MS	685:689	MS/MS	685:689	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	46	theme	HCD	654:656	arg1	spectrometry					671:682	collisional dissociation (HCD) tandem mass spectrometry	628:682	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	5	47	gly	glycoprotein	1156:1167	arg1	glycoprotein					1156:1167	a complex glycoprotein data set	1146:1176	a complex glycoprotein data set generated from human cancer cell line OVCAR3	1146:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	1	48	theme	complexity	241:250	arg1	microheterogeneity					204:221	microheterogeneity	204:221	microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	204:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	5	49	theme	sample	1105:1110	arg1	set					1090:1092	a data set	1083:1092	a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1083:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	49	theme	sample	1105:1110	arg1	sample					1105:1110	mixture sample	1097:1110	mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1097:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	50	theme	data	1169:1172	arg1	set					1174:1176	a complex glycoprotein data set	1146:1176	a complex glycoprotein data set generated from human cancer cell line OVCAR3	1146:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	2	51	from	potential	420:428	arg1	identification					437:450	the identification	433:450	the identification of glycopeptides	433:467	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	0	52	theme	Spectral	56:63	arg1	Search					73:78	Open Mass Spectral Library Search	46:78	Open Mass Spectral Library Search	46:78	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	3	53	theme	spectral	533:540	arg1	pMatchGlyco					514:524	pMatchGlyco	514:524	pMatchGlyco	514:524	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	53	theme	spectral	533:540	arg1	tool					557:560	a new spectral library search tool	527:560	a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	527:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	5	54	theme	pMatchGlyco	1050:1060	arg1	reliability					1035:1045	reliability	1035:1045	reliability	1035:1045	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	54	theme	pMatchGlyco	1050:1060	arg1	efficiency					1020:1029	efficiency	1020:1029	efficiency	1020:1029	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	3	55	theme	dissociation	640:651	arg1	MS/MS					685:689	MS/MS	685:689	MS/MS	685:689	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	55	theme	dissociation	640:651	arg1	spectrometry					671:682	collisional dissociation (HCD) tandem mass spectrometry	628:682	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	5	56	gly	glycoproteins	1128:1140	arg1	glycoproteins					1128:1140	six standard glycoproteins	1115:1140	six standard glycoproteins	1115:1140	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	4	57	theme	deglycopeptides	735:749	arg1	spectra					724:730	MS/MS spectra	718:730	(1) MS/MS spectra of deglycopeptides	714:749	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	0	58	theme	Mass	51:54	arg1	Search					73:78	Open Mass Spectral Library Search	46:78	Open Mass Spectral Library Search	46:78	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	3	59	theme	library	542:548	arg1	pMatchGlyco					514:524	pMatchGlyco	514:524	pMatchGlyco	514:524	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	59	theme	library	542:548	arg1	tool					557:560	a new spectral library search tool	527:560	a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	527:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	60	theme	new	529:531	arg1	pMatchGlyco					514:524	pMatchGlyco	514:524	pMatchGlyco	514:524	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	60	theme	new	529:531	arg1	tool					557:560	a new spectral library search tool	527:560	a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	527:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	5	61	theme	standard	1119:1126	arg1	glycoproteins					1128:1140	six standard glycoproteins	1115:1140	six standard glycoproteins	1115:1140	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	62	dep	efficiency	1020:1029	arg1	The					1016:1018	The	1016:1018	The	1016:1018	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	3	63	theme	spectrometry	671:682	arg1	data					692:695	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	4	64	used	used	755:758	arg2	spectra					724:730	MS/MS spectra	718:730	(1) MS/MS spectra of deglycopeptides	714:749	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	1	65	theme	challenging	137:147	arg1	task					149:152	a challenging task	135:152	a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones	135:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	65	theme	challenging	137:147	arg1	characterization					91:106	Confident characterization	81:106	Confident characterization of intact glycopeptides	81:130	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	66	theme	glycans	255:261	arg1	complexity					241:250	complexity	241:250	complexity of glycans	241:261	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	66	theme	glycans	255:261	arg1	fragmentation					281:293	insufficient fragmentation	268:293	insufficient fragmentation of peptide bones	268:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	1	66	theme	glycans	255:261	arg1	glycosylation					226:238	glycosylation	226:238	glycosylation	226:238	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30186849	3	67	theme	intact	566:571	arg1	identification					595:608	intact N-linked glycopeptide identification	566:608	intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	566:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	68	theme	N-linked	573:580	arg1	identification					595:608	intact N-linked glycopeptide identification	566:608	intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	566:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	4	69	theme	spectral	770:777	arg1	library					779:785	spectral library	770:785	spectral library	770:785	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	2	70	theme	promising	352:360	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	70	theme	promising	352:360	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	71	theme	computational	362:374	arg1	approach					376:383	a promising computational approach	350:383	a promising computational approach to peptide identification	350:409	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	2	71	theme	computational	362:374	arg1	search					340:345	Open mass spectral library search	313:345	Open mass spectral library search	313:345	Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored.					
30186849	3	72	theme	tandem	659:664	arg1	MS/MS					685:689	MS/MS	685:689	MS/MS	685:689	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	72	theme	tandem	659:664	arg1	spectrometry					671:682	collisional dissociation (HCD) tandem mass spectrometry	628:682	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	73	gly	glycopeptide	582:593	arg2	glycopeptide					582:593	intact N-linked glycopeptide identification	566:608	intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	566:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	5	74	theme	glycoproteins	1128:1140	arg1	sample					1105:1110	mixture sample	1097:1110	mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1097:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	75	theme	cancer	1199:1204	arg1	line					1211:1214	human cancer cell line OVCAR3	1193:1221	human cancer cell line OVCAR3	1193:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	0	76	theme	Library	65:71	arg1	Search					73:78	Open Mass Spectral Library Search	46:78	Open Mass Spectral Library Search	46:78	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.					
30186849	5	77	theme	data	1085:1088	arg1	set					1090:1092	a data set	1083:1092	a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1083:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	5	77	theme	data	1085:1088	arg1	sample					1105:1110	mixture sample	1097:1110	mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3	1097:1221	The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.					
30186849	3	78	theme	mass	666:669	arg1	MS/MS					685:689	MS/MS	685:689	MS/MS	685:689	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	3	78	theme	mass	666:669	arg1	spectrometry					671:682	collisional dissociation (HCD) tandem mass spectrometry	628:682	high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data	616:695	Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data.					
30186849	4	79	dep	open	867:870	arg1	tolerant					883:890	tolerant	883:890	tolerant	883:890	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	4	80	dep	spectra	724:730	arg1	1					715:715	1	715:715	1	715:715	In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold.					
30186849	1	81	theme	insufficient	268:279	arg1	fragmentation					281:293	insufficient fragmentation	268:293	insufficient fragmentation of peptide bones	268:310	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones.					
30259139	4	0	theme	secretory	957:965	arg1	pathways					967:974	the secretory pathways	953:974	the secretory pathways	953:974	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	7	1	theme	RF	1602:1603	arg1	N-glycome					1605:1613	the RF N-glycome	1598:1613	the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged	1598:1700	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	1	2	theme	conserved	121:129	arg1	organ					91:95	The subcommissural organ	72:95	The subcommissural organ (SCO)	72:101	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	2	theme	conserved	121:129	arg1	secreting					143:151	an ancient and conserved brain gland secreting	106:151	an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	106:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	3	theme	Reissner	212:219	arg1	fiber					221:225	the Reissner fiber	208:225	the Reissner fiber (RF)	208:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	3	theme	Reissner	212:219	arg1	RF					228:229	RF	228:229	RF	228:229	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	3	4	theme	antibodies	638:647	arg1	injection					625:633	injection	625:633	injection of antibodies against galectin-1 into the cerebrospinal fluid	625:695	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	4	theme	antibodies	638:647	arg1	CSF					620:622	the CSF	616:622	the CSF	616:622	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	8	5	theme	organization	1733:1744	arg1	model					1705:1709	A model	1703:1709	A model of the macromolecular organization of Reissner fiber	1703:1762	A model of the macromolecular organization of Reissner fiber is proposed.					
30259139	1	6	theme	brain	131:135	arg1	organ					91:95	The subcommissural organ	72:95	The subcommissural organ (SCO)	72:101	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	6	theme	brain	131:135	arg1	secreting					143:151	an ancient and conserved brain gland secreting	106:151	an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	106:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	3	7	theme	mass	793:796	arg1	spectrometry					798:809	mass spectrometry	793:809	mass spectrometry	793:809	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	8	used	used	763:766	arg2	RF					756:757	isolated bovine RF	740:757	isolated bovine RF	740:757	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	1	9	gly	glycoproteins	184:196	arg1	glycoproteins					184:196	cerebrospinal fluid (CSF) glycoproteins	158:196	cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	158:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	2	10	theme	present	237:243	arg1	investigation					245:257	The present investigation	233:257	The present investigation	233:257	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	7	11	theme	potential	1640:1648	arg1	functions					1650:1658	the potential functions	1636:1658	the potential functions of RF	1636:1664	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	3	12	theme	proteome	772:779	arg1	analyses					781:788	proteome analyses	772:788	proteome analyses	772:788	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	5	13	dep	in	1274:1275	arg1	vivo					1277:1280	vivo	1277:1280	vivo	1277:1280	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	8	14	theme	Reissner	1749:1756	arg1	fiber					1758:1762	Reissner fiber	1749:1762	Reissner fiber	1749:1762	A model of the macromolecular organization of Reissner fiber is proposed.					
30259139	4	15	theme	molecules	929:937	arg1	pool					909:912	a small pool	901:912	a small pool of SCO-spondin molecules	901:937	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	2	16	gly	glycoproteins	341:353	arg1	glycoproteins					341:353	RF glycoproteins	338:353	RF glycoproteins	338:353	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	7	17	theme	RF	1663:1664	arg1	functions					1650:1658	the potential functions	1636:1658	the potential functions of RF	1636:1664	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	1	18	theme	gland	137:141	arg1	organ					91:95	The subcommissural organ	72:95	The subcommissural organ (SCO)	72:101	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	18	theme	gland	137:141	arg1	secreting					143:151	an ancient and conserved brain gland secreting	106:151	an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	106:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	4	19	theme	SCO-spondin	917:927	arg1	molecules					929:937	SCO-spondin molecules	917:937	SCO-spondin molecules	917:937	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	2	20	theme	RF	415:416	arg1	proteome					418:425	the RF proteome	411:425	the RF proteome	411:425	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	5	21	theme	anti-galectin-1	1289:1303	arg1	use					1282:1284	the in vivo use	1270:1284	the in vivo use of anti-galectin-1	1270:1303	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	2	22	theme	glycoproteins	341:353	arg1	dynamic					299:305	dynamic	299:305	dynamic	299:305	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	0	23	theme	subcommissural	4:17	arg1	organ					19:23	The subcommissural organ	0:23	The subcommissural organ	0:23	The subcommissural organ and the Reissner fiber: old friends revisited.					
30259139	3	24	theme	cerebrospinal	677:689	arg1	fluid					691:695	the cerebrospinal fluid	673:695	the cerebrospinal fluid	673:695	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	5	25	theme	RF	1362:1363	arg1	assembly					1350:1357	the assembly	1346:1357	the assembly of RF	1346:1363	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	3	26	theme	Various	503:509	arg1	approaches					526:535	Various methodological approaches	503:535	Various methodological approaches	503:535	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	6	27	theme	multiciliated	1459:1471	arg1	cells					1483:1487	multiciliated ependymal cells	1459:1487	multiciliated ependymal cells lying close to the SCO	1459:1510	Galectin-1 is not secreted by the SCO but evidence was obtained that it would be secreted by multiciliated ependymal cells lying close to the SCO.					
30259139	2	28	theme	RF	338:339	arg1	glycoproteins					341:353	RF glycoproteins	338:353	RF glycoproteins	338:353	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	4	29	theme	small	903:907	arg1	pool					909:912	a small pool	901:912	a small pool of SCO-spondin molecules	901:937	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	3	30	theme	electron	708:715	arg1	CSF					620:622	the CSF	616:622	the CSF	616:622	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	30	theme	electron	708:715	arg1	methods					731:737	light and electron microscopical methods	698:737	light and electron microscopical methods	698:737	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	1	31	theme	subcommissural	76:89	arg1	SCO					98:100	SCO	98:100	SCO	98:100	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	31	theme	subcommissural	76:89	arg1	organ					91:95	The subcommissural organ	72:95	The subcommissural organ (SCO)	72:101	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	31	theme	subcommissural	76:89	arg1	secreting					143:151	an ancient and conserved brain gland secreting	106:151	an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	106:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	32	theme	cerebrospinal	158:170	arg1	CSF					179:181	CSF	179:181	CSF	179:181	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	32	theme	cerebrospinal	158:170	arg1	fluid					172:176	cerebrospinal fluid	158:176	cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	158:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	7	33	theme	structures	1568:1577	arg1	complexity					1547:1556	complexity	1547:1556	complexity of glycan structures	1547:1577	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	7	33	theme	structures	1568:1577	arg1	structures					1568:1577	glycan structures	1561:1577	glycan structures	1561:1577	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	7	33	theme	structures	1568:1577	arg1	variety					1535:1541	a surprising variety	1522:1541	a surprising variety	1522:1541	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	3	34	theme	microscopical	717:729	arg1	CSF					620:622	the CSF	616:622	the CSF	616:622	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	34	theme	microscopical	717:729	arg1	methods					731:737	light and electron microscopical methods	698:737	light and electron microscopical methods	698:737	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	2	35	theme	RF	485:486	arg1	glycoproteins					488:500	RF glycoproteins	485:500	RF glycoproteins	485:500	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	4	36	theme	endoplasmic	1061:1071	arg1	reticulum					1073:1081	the rough endoplasmic reticulum	1051:1081	the rough endoplasmic reticulum for hours or days	1051:1099	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	1	37	theme	fluid	172:176	arg1	glycoproteins					184:196	cerebrospinal fluid (CSF) glycoproteins	158:196	cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	158:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	3	38	theme	methodological	511:524	arg1	approaches					526:535	Various methodological approaches	503:535	Various methodological approaches	503:535	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	39	theme	light	698:702	arg1	CSF					620:622	the CSF	616:622	the CSF	616:622	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	39	theme	light	698:702	arg1	methods					731:737	light and electron microscopical methods	698:737	light and electron microscopical methods	698:737	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	5	40	theme	in	1274:1275	arg1	use					1282:1284	the in vivo use	1270:1284	the in vivo use of anti-galectin-1	1270:1303	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	2	41	theme	process	327:333	arg1	dynamic					299:305	dynamic	299:305	dynamic	299:305	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	0	42	theme	Reissner	33:40	arg1	friends					53:59	the Reissner fiber: old friends	29:59	the Reissner fiber: old friends	29:59	The subcommissural organ and the Reissner fiber: old friends revisited.					
30259139	7	43	theme	glycan	1561:1566	arg1	structures					1568:1577	glycan structures	1561:1577	glycan structures	1561:1577	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	2	44	theme	assembly	473:480	arg1	mechanism					460:468	the mechanism	456:468	the mechanism of assembly of RF glycoproteins	456:500	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	8	45	theme	fiber	1758:1762	arg1	organization					1733:1744	the macromolecular organization	1714:1744	the macromolecular organization of Reissner fiber	1714:1762	A model of the macromolecular organization of Reissner fiber is proposed.					
30259139	3	46	theme	isolated	740:747	arg1	RF					756:757	isolated bovine RF	740:757	isolated bovine RF	740:757	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	3	47	theme	bovine	749:754	arg1	RF					756:757	isolated bovine RF	740:757	isolated bovine RF	740:757	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	4	48	theme	labelling	862:870	arg1	study					872:876	The biosynthetic labelling study	845:876	The biosynthetic labelling study	845:876	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	0	49	theme	fiber	42:46	arg1	friends					53:59	the Reissner fiber: old friends	29:59	the Reissner fiber: old friends	29:59	The subcommissural organ and the Reissner fiber: old friends revisited.					
30259139	7	50	theme	surprising	1524:1533	arg1	structures					1568:1577	glycan structures	1561:1577	glycan structures	1561:1577	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	7	50	theme	surprising	1524:1533	arg1	variety					1535:1541	a surprising variety	1522:1541	a surprising variety	1522:1541	Further, a surprising variety and complexity of glycan structures were identified in the RF N-glycome that further expands the potential functions of RF to a level not previously envisaged.					
30259139	4	51	theme	biosynthetic	849:860	arg1	study					872:876	The biosynthetic labelling study	845:876	The biosynthetic labelling study	845:876	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	3	52	theme	glycome	815:821	arg1	analysis					823:830	glycome analysis	815:830	glycome analysis by xCGE-LIF	815:842	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	8	53	theme	macromolecular	1718:1731	arg1	organization					1733:1744	the macromolecular organization	1714:1744	the macromolecular organization of Reissner fiber	1714:1762	A model of the macromolecular organization of Reissner fiber is proposed.					
30259139	2	54	gly	glycoproteins	488:500	arg1	glycoproteins					488:500	RF glycoproteins	485:500	RF glycoproteins	485:500	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	5	55	theme	proteomic	1183:1191	arg1	analysis					1193:1200	The proteomic analysis	1179:1200	The proteomic analysis of RF	1179:1206	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	2	56	theme	glycoproteins	488:500	arg1	assembly					473:480	assembly	473:480	assembly of RF glycoproteins	473:500	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	4	57	theme	secretory	1121:1129	arg1	pathway					1131:1137	the secretory pathway	1117:1137	the secretory pathway	1117:1137	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	3	58	used	used	542:545	arg2	approaches					526:535	Various methodological approaches	503:535	Various methodological approaches	503:535	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	4	59	theme	rough	1055:1059	arg1	reticulum					1073:1081	the rough endoplasmic reticulum	1051:1081	the rough endoplasmic reticulum for hours or days	1051:1099	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	6	60	theme	ependymal	1473:1481	arg1	cells					1483:1487	multiciliated ependymal cells	1459:1487	multiciliated ependymal cells lying close to the SCO	1459:1510	Galectin-1 is not secreted by the SCO but evidence was obtained that it would be secreted by multiciliated ependymal cells lying close to the SCO.					
30259139	3	61	theme	biosynthetic	548:559	arg1	labelling					561:569	biosynthetic labelling	548:569	biosynthetic labelling	548:569	Various methodological approaches were used: biosynthetic labelling injecting 35S-cysteine and 3H-galactose into the CSF, injection of antibodies against galectin-1 into the cerebrospinal fluid, light and electron microscopical methods; isolated bovine RF was used for proteome analyses by mass spectrometry and glycome analysis by xCGE-LIF.					
30259139	1	62	theme	ancient	109:115	arg1	organ					91:95	The subcommissural organ	72:95	The subcommissural organ (SCO)	72:101	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	1	62	theme	ancient	109:115	arg1	secreting					143:151	an ancient and conserved brain gland secreting	106:151	an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF)	106:230	The subcommissural organ (SCO) is an ancient and conserved brain gland secreting into cerebrospinal fluid (CSF) glycoproteins that form the Reissner fiber (RF).					
30259139	5	63	theme	SCO-spondin	1257:1267	arg1	partners					1245:1252	partners	1245:1252	partners of SCO-spondin	1245:1267	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	5	63	theme	SCO-spondin	1257:1267	arg1	galectin-1					1231:1240	galectin-1	1231:1240	galectin-1	1231:1240	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	5	63	theme	SCO-spondin	1257:1267	arg1	clusterin					1217:1225	clusterin	1217:1225	clusterin	1217:1225	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
30259139	0	64	theme	old	49:51	arg1	friends					53:59	the Reissner fiber: old friends	29:59	the Reissner fiber: old friends	29:59	The subcommissural organ and the Reissner fiber: old friends revisited.					
30259139	2	65	theme	biosynthetic	314:325	arg1	process					327:333	the biosynthetic process	310:333	the biosynthetic process	310:333	The present investigation was designed to further investigate the dynamic of the biosynthetic process of RF glycoproteins prior and after their release into the CSF, to identify the RF proteome and N-glycome and to clarify the mechanism of assembly of RF glycoproteins.					
30259139	4	66	theme	SCO-spondin	1015:1025	arg1	molecules					1027:1035	the SCO-spondin molecules	1011:1035	the SCO-spondin molecules	1011:1035	The biosynthetic labelling study further supported that a small pool of SCO-spondin molecules rapidly enter the secretory pathways after its synthesis, while most of the SCO-spondin molecules are stored in the rough endoplasmic reticulum for hours or days before entering the secretory pathway and being released to assemble into RF.					
30259139	5	67	theme	RF	1205:1206	arg1	analysis					1193:1200	The proteomic analysis	1179:1200	The proteomic analysis of RF	1179:1206	The proteomic analysis of RF revealed clusterin and galectin-1 as partners of SCO-spondin; the in vivo use of anti-galectin-1 showed that this lectin is essential for the assembly of RF.					
29947113	6	0	theme	Endo-β-N-acetylglucosaminidase	691:720	arg1	H					722:722	Endo-β-N-acetylglucosaminidase H	691:722	Endo-β-N-acetylglucosaminidase H	691:722	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	4	1	theme	Several	388:394	arg1	methods					396:402	Several methods	388:402	Several methods	388:402	Several methods have been proposed to characterize the glycosylation changes.					
29947113	1	2	theme	genetic	142:148	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	3	3	theme	mannose	348:354	arg1	residue					356:362	the terminal mannose residue	335:362	the terminal mannose residue from the middle branch	335:385	MAN1B1 encodes an α1,2-mannosidase that removes the terminal mannose residue from the middle branch.					
29947113	2	4	theme	CDG-II	279:284	arg1	causes					269:274	the more frequent causes	251:274	the more frequent causes of CDG-II	251:284	With some 33 reported patients, MAN1B1-CDG belongs to the more frequent causes of CDG-II.					
29947113	5	5	theme	abnormal	485:492	arg1	structures					516:525	the abnormal accumulating N-glycan structures	481:525	the abnormal accumulating N-glycan structures	481:525	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	2	6	theme	frequent	260:267	arg1	causes					269:274	the more frequent causes	251:274	the more frequent causes of CDG-II	251:284	With some 33 reported patients, MAN1B1-CDG belongs to the more frequent causes of CDG-II.					
29947113	5	7	theme	accumulating	494:505	arg1	structures					516:525	the abnormal accumulating N-glycan structures	481:525	the abnormal accumulating N-glycan structures	481:525	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	1	8	theme	protein	150:156	arg1	group					133:137	heterogeneous group	119:137	heterogeneous group of genetic protein and lipid glycosylation abnormalities	119:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	8	theme	protein	150:156	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	8	theme	protein	150:156	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	8	theme	protein	150:156	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	3	9	theme	terminal	339:346	arg1	residue					356:362	the terminal mannose residue	335:362	the terminal mannose residue from the middle branch	335:385	MAN1B1 encodes an α1,2-mannosidase that removes the terminal mannose residue from the middle branch.					
29947113	5	10	theme	N-glycan	507:514	arg1	structures					516:525	the abnormal accumulating N-glycan structures	481:525	the abnormal accumulating N-glycan structures	481:525	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	3	11	theme	middle	373:378	arg1	branch					380:385	the middle branch	369:385	the middle branch	369:385	MAN1B1 encodes an α1,2-mannosidase that removes the terminal mannose residue from the middle branch.					
29947113	3	12	from	branch	380:385	arg1	residue					356:362	the terminal mannose residue	335:362	the terminal mannose residue from the middle branch	335:385	MAN1B1 encodes an α1,2-mannosidase that removes the terminal mannose residue from the middle branch.					
29947113	5	13	theme	total	574:578	arg1	serum					586:590	total human serum	574:590	total human serum	574:590	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	1	14	theme	Congenital	71:80	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	14	theme	Congenital	71:80	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	14	theme	Congenital	71:80	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	14	theme	Congenital	71:80	arg1	group					133:137	heterogeneous group	119:137	heterogeneous group of genetic protein and lipid glycosylation abnormalities	119:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	15	theme	lipid	162:166	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	5	16	theme	human	580:584	arg1	serum					586:590	total human serum	574:590	total human serum	574:590	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	1	17	theme	glycosylation	168:180	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	0	18	theme	H	23:23	arg1	Use					0:2	Use	0:2	Use of Endoglycosidase H as a diagnostic tool for MAN1B1-CDG patients.	0:69	Use of Endoglycosidase H as a diagnostic tool for MAN1B1-CDG patients.					
29947113	6	19	theme	mannosidase	767:777	arg1	defects					779:785	mannosidase defects	767:785	mannosidase defects	767:785	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	1	20	theme	abnormalities	182:194	arg1	group					133:137	heterogeneous group	119:137	heterogeneous group of genetic protein and lipid glycosylation abnormalities	119:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	20	theme	abnormalities	182:194	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	20	theme	abnormalities	182:194	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	20	theme	abnormalities	182:194	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	0	21	theme	Endoglycosidase	7:21	arg1	H					23:23	Endoglycosidase H	7:23	Endoglycosidase H	7:23	Use of Endoglycosidase H as a diagnostic tool for MAN1B1-CDG patients.					
29947113	6	22	theme	MAN1B1-CDG	743:752	arg1	patients					754:761	MAN1B1-CDG patients	743:761	MAN1B1-CDG patients	743:761	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	4	23	theme	glycosylation	443:455	arg1	changes					457:463	the glycosylation changes	439:463	the glycosylation changes	439:463	Several methods have been proposed to characterize the glycosylation changes.					
29947113	1	24	theme	glycosylation	95:107	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	24	theme	glycosylation	95:107	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	24	theme	glycosylation	95:107	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	24	theme	glycosylation	95:107	arg1	group					133:137	heterogeneous group	119:137	heterogeneous group of genetic protein and lipid glycosylation abnormalities	119:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	6	25	theme	straightforward	645:659	arg1	procedure					661:669	a straightforward procedure	643:669	a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects	643:785	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	5	26	from	absent	538:543	arg1	MAN1B1-CDG					469:478	MAN1B1-CDG	469:478	MAN1B1-CDG	469:478	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	2	27	theme	reported	210:217	arg1	patients					219:226	some 33 reported patients	202:226	some 33 reported patients	202:226	With some 33 reported patients, MAN1B1-CDG belongs to the more frequent causes of CDG-II.					
29947113	0	28	theme	diagnostic	30:39	arg1	tool					41:44	a diagnostic tool	28:44	a diagnostic tool for MAN1B1-CDG patients	28:68	Use of Endoglycosidase H as a diagnostic tool for MAN1B1-CDG patients.					
29947113	6	29	attach	present	635:641	arg2	we					632:633	we	632:633	we	632:633	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	6	29	attach	present	635:641	arg1	study					625:629	this study	620:629	this study	620:629	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29947113	5	30	from	MAN1B1-CDG	469:478	arg1	absent					538:543	absent	538:543	absent	538:543	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	5	31	theme	trace	557:561	arg1	amounts					563:569	trace amounts	557:569	trace amounts	557:569	In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum.					
29947113	0	32	theme	MAN1B1-CDG	50:59	arg1	patients					61:68	MAN1B1-CDG patients	50:68	MAN1B1-CDG patients	50:68	Use of Endoglycosidase H as a diagnostic tool for MAN1B1-CDG patients.					
29947113	1	33	theme	heterogeneous	119:131	arg1	group					133:137	heterogeneous group	119:137	heterogeneous group of genetic protein and lipid glycosylation abnormalities	119:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	33	theme	heterogeneous	119:131	arg1	protein					150:156	genetic protein	142:156	genetic protein	142:156	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	33	theme	heterogeneous	119:131	arg1	abnormalities					182:194	lipid glycosylation abnormalities	162:194	lipid glycosylation abnormalities	162:194	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	1	33	theme	heterogeneous	119:131	arg1	disorders					82:90	Congenital disorders	71:90	Congenital disorders of glycosylation (CDG)	71:113	Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities.					
29947113	6	34	theme	H	722:722	arg1	use					684:686	the use	680:686	the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects	680:785	To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo-β-N-acetylglucosaminidase H to easily diagnose MAN1B1-CDG patients and mannosidase defects.					
29446564	7	0	theme	parasite-vector	1247:1261	arg1	interactions					1263:1274	parasite-vector interactions	1247:1274	parasite-vector interactions	1247:1274	We propose that these molecules could be involved in the process of parasite-vector interactions.					
29446564	3	1	gly	glycoproteins	400:412	arg1	glycoproteins					400:412	several glycoproteins	392:412	several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis	392:509	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	6	2	theme	glycoproteins	1070:1082	arg1	glycoproteins					1070:1082	glycoproteins	1070:1082	glycoproteins with different sugar residues	1070:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	2	theme	glycoproteins	1070:1082	arg1	high-mannose					1134:1145	abundant high-mannose	1125:1145	abundant high-mannose	1125:1145	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	2	theme	glycoproteins	1070:1082	arg1	variety					1059:1065	a wide variety	1052:1065	a wide variety	1052:1065	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	2	theme	glycoproteins	1070:1082	arg1	complex					1151:1157	complex	1151:1157	complex	1151:1157	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	3	theme	abundant	1125:1132	arg1	high-mannose					1134:1145	abundant high-mannose	1125:1145	abundant high-mannose	1125:1145	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	4	4	from	tissues	692:698	arg1	extracts					661:668	glycoprotein extracts	648:668	glycoprotein extracts from these intestinal tissues	648:698	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	6	5	theme	PMM	1029:1031	arg1	extracts					1033:1040	PMM extracts	1029:1040	PMM extracts	1029:1040	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	0	6	theme	Triatoma	75:82	arg1	pallidipennis					93:105	Triatoma (Meccus) pallidipennis	75:105	Triatoma (Meccus) pallidipennis	75:105	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	4	7	gly	glycoprotein	648:659	arg1	glycoprotein					648:659	glycoprotein extracts	648:668	glycoprotein extracts from these intestinal tissues	648:698	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	3	8	theme	Meccus	489:494	arg1	pallidipennis					497:509	Triatoma (Meccus) pallidipennis	479:509	Triatoma (Meccus) pallidipennis	479:509	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	5	9	theme	different	993:1001	arg1	conditions					1017:1026	different physiological conditions	993:1026	different physiological conditions	993:1026	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	0	10	from	Glycosylation	0:12	arg1	proteins					17:24	proteins	17:24	proteins	17:24	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	3	11	theme	feeding	527:533	arg1	conditions					535:544	different feeding conditions	517:544	different feeding conditions	517:544	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	6	12	with	glycoproteins	1070:1082	arg1	residues					1105:1112	different sugar residues	1089:1112	different sugar residues	1089:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	4	13	theme	protein-linked	577:590	arg1	glycans					592:598	protein-linked glycans	577:598	protein-linked glycans	577:598	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	0	14	theme	Meccus	85:90	arg1	pallidipennis					93:105	Triatoma (Meccus) pallidipennis	75:105	Triatoma (Meccus) pallidipennis	75:105	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	5	15	dep	different	993:1001	arg1	physiological					1003:1015	physiological	1003:1015	physiological	1003:1015	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	6	16	contain	contained	1042:1050	arg2	variety					1059:1065	a wide variety	1052:1065	a wide variety	1052:1065	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	16	contain	contained	1042:1050	arg2	high-mannose					1134:1145	abundant high-mannose	1125:1145	abundant high-mannose	1125:1145	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	16	contain	contained	1042:1050	arg2	complex					1151:1157	complex	1151:1157	complex	1151:1157	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	16	contain	contained	1042:1050	arg1	extracts					1033:1040	PMM extracts	1029:1040	PMM extracts	1029:1040	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	16	contain	contained	1042:1050	arg2	glycoproteins					1070:1082	glycoproteins	1070:1082	glycoproteins with different sugar residues	1070:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	1	17	theme	insect	248:253	arg1	vector					255:260	its insect vector to multiply and reach the infective stage	244:302	its insect vector to multiply and reach the infective stage	244:302	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	4	18	from	changes	566:572	arg1	glycans					592:598	protein-linked glycans	577:598	protein-linked glycans	577:598	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	4	19	theme	lectin	614:619	arg1	analyses					636:643	lectin and immunoblot analyses	614:643	analyses	636:643	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	4	20	theme	wide	782:785	arg1	range					787:791	a wide range	780:791	a wide range of different glycans epitopes	780:821	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	5	21	theme	proteins	871:878	arg1	amount					845:850	amount	845:850	amount	845:850	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	21	theme	proteins	871:878	arg1	glycoproteins					884:896	glycoproteins	884:896	glycoproteins	884:896	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	21	theme	proteins	871:878	arg1	proteins					871:878	proteins	871:878	proteins	871:878	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	21	theme	proteins	871:878	arg1	composition					856:866	composition	856:866	composition	856:866	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	4	22	theme	glycoprotein	648:659	arg1	extracts					661:668	glycoprotein extracts	648:668	glycoprotein extracts from these intestinal tissues	648:698	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	0	23	theme	pallidipennis	93:105	arg1	intestine					33:41	intestine	33:41	intestine	33:41	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	0	23	theme	pallidipennis	93:105	arg1	membrane					63:70	perimicrovillar membrane	47:70	perimicrovillar membrane	47:70	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	3	24	theme	several	392:398	arg1	glycoproteins					400:412	several glycoproteins	392:412	several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis	392:509	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	3	25	theme	different	517:525	arg1	conditions					535:544	different feeding conditions	517:544	different feeding conditions	517:544	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	1	26	theme	causative	167:175	arg1	agent					177:181	the causative agent	163:181	the causative agent of Chagas disease	163:199	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	1	26	theme	causative	167:175	arg1	cruzi					156:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	7	27	theme	interactions	1263:1274	arg1	process					1236:1242	the process	1232:1242	the process of parasite-vector interactions	1232:1274	We propose that these molecules could be involved in the process of parasite-vector interactions.					
29446564	2	28	theme	midgut-specific	353:367	arg1	glycans					369:375	midgut-specific glycans	353:375	midgut-specific glycans	353:375	Many studies suggest that the parasite binds to midgut-specific glycans.					
29446564	1	29	theme	vector	255:260	arg1	midgut					234:239	the midgut	230:239	the midgut of its insect vector to multiply and reach the infective stage	230:302	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	6	30	gly	sialylated	1159:1168	arg1	glycans					1170:1176	sialylated glycans	1159:1176	sialylated glycans	1159:1176	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	4	31	from	analyses	636:643	arg1	extracts					661:668	glycoprotein extracts	648:668	glycoprotein extracts from these intestinal tissues	648:698	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	6	32	theme	different	1089:1097	arg1	residues					1105:1112	different sugar residues	1089:1112	different sugar residues	1089:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	0	33	theme	different	114:122	arg1	conditions					132:141	different feeding conditions	114:141	different feeding conditions	114:141	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	6	34	dep	high-mannose	1134:1145	arg1	glycans					1170:1176	sialylated glycans	1159:1176	sialylated glycans	1159:1176	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	1	35	theme	Chagas	186:191	arg1	disease					193:199	Chagas disease	186:199	Chagas disease	186:199	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	6	36	gly	glycoproteins	1070:1082	arg1	glycoproteins					1070:1082	glycoproteins	1070:1082	glycoproteins with different sugar residues	1070:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	5	37	theme	different	914:922	arg1	structures					932:941	different glycans structures	914:941	different glycans structures	914:941	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	1	38	theme	disease	193:199	arg1	agent					177:181	the causative agent	163:181	the causative agent of Chagas disease	163:199	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	1	38	theme	disease	193:199	arg1	cruzi					156:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	0	39	theme	intestine	33:41	arg1	proteins					17:24	proteins	17:24	proteins	17:24	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	4	40	theme	well-characterized	706:723	arg1	lectins					725:731	well-characterized lectins	706:731	well-characterized lectins	706:731	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	6	41	theme	sugar	1099:1103	arg1	residues					1105:1112	different sugar residues	1089:1112	different sugar residues	1089:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	0	42	theme	feeding	124:130	arg1	conditions					132:141	different feeding conditions	114:141	different feeding conditions	114:141	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	3	43	theme	perimicrovillar	445:459	arg1	PMM					471:473	perimicrovillar membrane (PMM)	445:474	perimicrovillar membrane (PMM)	445:474	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	0	44	theme	perimicrovillar	47:61	arg1	membrane					63:70	perimicrovillar membrane	47:70	perimicrovillar membrane	47:70	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	5	45	theme	glycoproteins	884:896	arg1	amount					845:850	amount	845:850	amount	845:850	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	45	theme	glycoproteins	884:896	arg1	glycoproteins					884:896	glycoproteins	884:896	glycoproteins	884:896	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	45	theme	glycoproteins	884:896	arg1	proteins					871:878	proteins	871:878	proteins	871:878	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	5	45	theme	glycoproteins	884:896	arg1	composition					856:866	composition	856:866	composition	856:866	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	0	46	dep	intestine	33:41	arg1	the					29:31	the	29:31	the	29:31	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	5	47	theme	glycans	924:930	arg1	structures					932:941	different glycans structures	914:941	different glycans structures	914:941	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	1	48	theme	infective	288:296	arg1	stage					298:302	the infective stage	284:302	the infective stage	284:302	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	5	49	gly	glycoproteins	884:896	arg1	glycoproteins					884:896	glycoproteins	884:896	glycoproteins	884:896	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	4	50	link	protein-linked	577:590	arg1	glycans					592:598	protein-linked glycans	577:598	protein-linked glycans	577:598	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	3	51	dep	intestine	431:439	arg1	the					427:429	the	427:429	the	427:429	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	3	52	theme	Triatoma	479:486	arg1	pallidipennis					497:509	Triatoma (Meccus) pallidipennis	479:509	Triatoma (Meccus) pallidipennis	479:509	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	5	53	dep	amount	845:850	arg1	the					841:843	the	841:843	the	841:843	We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.					
29446564	2	54	theme	Many	305:308	arg1	studies					310:316	Many studies	305:316	Many studies	305:316	Many studies suggest that the parasite binds to midgut-specific glycans.					
29446564	6	55	theme	wide	1054:1057	arg1	glycoproteins					1070:1082	glycoproteins	1070:1082	glycoproteins with different sugar residues	1070:1112	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	55	theme	wide	1054:1057	arg1	high-mannose					1134:1145	abundant high-mannose	1125:1145	abundant high-mannose	1125:1145	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	55	theme	wide	1054:1057	arg1	variety					1059:1065	a wide variety	1052:1065	a wide variety	1052:1065	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	6	55	theme	wide	1054:1057	arg1	complex					1151:1157	complex	1151:1157	complex	1151:1157	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	1	56	from	molecules	217:225	arg1	midgut					234:239	the midgut	230:239	the midgut of its insect vector to multiply and reach the infective stage	230:302	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	4	57	theme	range	787:791	arg1	epitopes					814:821	a wide range of different glycans epitopes	780:821	a wide range of different glycans epitopes	780:821	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	3	58	theme	pallidipennis	497:509	arg1	PMM					471:473	perimicrovillar membrane (PMM)	445:474	perimicrovillar membrane (PMM)	445:474	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	3	58	theme	pallidipennis	497:509	arg1	intestine					431:439	intestine	431:439	intestine	431:439	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	6	59	theme	sialylated	1159:1168	arg1	glycans					1170:1176	sialylated glycans	1159:1176	sialylated glycans	1159:1176	PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans.					
29446564	4	60	theme	immunoblot	625:634	arg1	analyses					636:643	lectin and immunoblot analyses	614:643	analyses	636:643	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	4	61	theme	glycans	806:812	arg1	range					787:791	a wide range	780:791	a wide range of different glycans epitopes	780:821	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	3	62	theme	membrane	461:468	arg1	PMM					471:473	perimicrovillar membrane (PMM)	445:474	perimicrovillar membrane (PMM)	445:474	We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.					
29446564	4	63	theme	intestinal	681:690	arg1	tissues					692:698	these intestinal tissues	675:698	these intestinal tissues	675:698	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	0	64	theme	membrane	63:70	arg1	proteins					17:24	proteins	17:24	proteins	17:24	Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions.					
29446564	4	65	theme	different	796:804	arg1	glycans					806:812	different glycans	796:812	different glycans	796:812	In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes.					
29446564	1	66	theme	Trypanosoma	144:154	arg1	agent					177:181	the causative agent	163:181	the causative agent of Chagas disease	163:199	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
29446564	1	66	theme	Trypanosoma	144:154	arg1	cruzi					156:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi	144:160	Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.					
30351481	6	0	theme	glycans	1310:1316	arg1	synthesis					1263:1271	the first synthesis	1253:1271	the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1253:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	2	1	theme	acid	614:617	arg1	monosaccharides					393:407	eight or nine monosaccharides	379:407	eight or nine monosaccharides	379:407	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	1	theme	acid	614:617	arg1	residues					625:632	3-deoxy-d-manno-2-octulosonic acid (KDO) residues	584:632	3-deoxy-d-manno-2-octulosonic acid (KDO) residues	584:632	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	4	2	theme	key	783:785	arg1	components					787:796	key components	783:796	key components of the LOSs	783:808	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	5	3	theme	suitable	1129:1136	arg1	reagent					1150:1156	a suitable α-directing reagent	1127:1156	a suitable α-directing reagent	1127:1156	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	3	theme	suitable	1129:1136	arg1	derivative					1061:1070	a 2,3-ene derivative	1051:1070	a 2,3-ene derivative of KDO	1051:1077	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	6	4	theme	challenging	1289:1299	arg1	glycans					1310:1316	structurally challenging LOS core glycans	1276:1316	structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1276:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	0	5	theme	Putative	92:99	arg1	Cause					101:105	A Putative Cause	90:105	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.	0:133	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	5	6	theme	phenylselenyl	1084:1096	arg1	trifluoromethanesulfonate					1098:1122	phenylselenyl trifluoromethanesulfonate	1084:1122	phenylselenyl trifluoromethanesulfonate	1084:1122	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	1	7	theme	Guillain-Barré	278:291	arg1	syndrome					293:300	Guillain-Barré syndrome	278:300	Guillain-Barré syndrome	278:300	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	0	8	theme	Guillain-Barré	110:123	arg1	Syndrome					125:132	Guillain-Barré Syndrome	110:132	Guillain-Barré Syndrome	110:132	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	6	9	theme	ganglioside	1329:1339	arg1	GM1					1341:1343	ganglioside GM1	1329:1343	ganglioside GM1	1329:1343	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	5	10	theme	difficult	973:981	arg1	constructions					1011:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	1	11	theme	branched	172:179	arg1	oligosaccharides					186:201	the highly branched core oligosaccharides	161:201	the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni	161:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	2	12	theme	building	451:458	arg1	blocks					460:465	key building blocks	447:465	key building blocks	447:465	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	12	theme	building	451:458	arg1	monosaccharides					393:407	eight or nine monosaccharides	379:407	eight or nine monosaccharides	379:407	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	4	13	theme	di-O-isopropylidene-protected	865:893	arg1	d-mannose					895:903	di-O-isopropylidene-protected d-mannose	865:903	di-O-isopropylidene-protected d-mannose	865:903	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	1	14	theme	core	181:184	arg1	oligosaccharides					186:201	the highly branched core oligosaccharides	161:201	the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni	161:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	2	15	theme	key	447:449	arg1	blocks					460:465	key building blocks	447:465	key building blocks	447:465	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	15	theme	key	447:449	arg1	monosaccharides					393:407	eight or nine monosaccharides	379:407	eight or nine monosaccharides	379:407	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	0	16	from	Oligosaccharides	22:37	arg1	jejuni					82:87	Campylobacter jejuni	68:87	Campylobacter jejuni	68:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	5	17	theme	2,3-ene	1053:1059	arg1	reagent					1150:1156	a suitable α-directing reagent	1127:1156	a suitable α-directing reagent	1127:1156	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	17	theme	2,3-ene	1053:1059	arg1	derivative					1061:1070	a 2,3-ene derivative	1051:1070	a 2,3-ene derivative of KDO	1051:1077	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	6	18	theme	synthetic	1218:1226	arg1	route					1228:1232	a convergent synthetic route	1205:1232	a convergent synthetic route	1205:1232	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	1	19	theme	oligosaccharides	186:201	arg1	synthesis					148:156	The chemical synthesis	135:156	The chemical synthesis	135:156	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	4	20	theme	LOSs	805:808	arg1	components					787:796	key components	783:796	key components of the LOSs	783:808	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	6	21	theme	core	1305:1308	arg1	glycans					1310:1316	structurally challenging LOS core glycans	1276:1316	structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1276:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	4	22	theme	KDO	766:768	arg1	residues					770:777	Less obtainable l-glycero-d-manno-heptose and KDO residues	720:777	residues	770:777	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	0	23	theme	Core	17:20	arg1	Oligosaccharides					22:37	the Core Oligosaccharides	13:37	the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni	13:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	4	24	theme	p-methoxyphenyl	833:847	arg1	d-mannoside					849:859	p-methoxyphenyl d-mannoside	833:859	p-methoxyphenyl d-mannoside	833:859	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	1	25	theme	lipooligosaccharides	206:225	arg1	oligosaccharides					186:201	the highly branched core oligosaccharides	161:201	the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni	161:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	5	26	theme	glycoside	943:951	arg1	one					957:959	one	957:959	one	957:959	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	26	theme	glycoside	943:951	arg1	constructions					1011:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	26	theme	glycoside	943:951	arg1	synthesis					924:932	The synthesis	920:932	The synthesis of α-KDO glycoside	920:951	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	0	27	theme	Syndrome	125:132	arg1	Cause					101:105	A Putative Cause	90:105	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.	0:133	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	5	28	theme	KDO	1075:1077	arg1	reagent					1150:1156	a suitable α-directing reagent	1127:1156	a suitable α-directing reagent	1127:1156	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	28	theme	KDO	1075:1077	arg1	derivative					1061:1070	a 2,3-ene derivative	1051:1070	a 2,3-ene derivative of KDO	1051:1077	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	2	29	theme	ganglioside-core	468:483	arg1	GM1-/GD1a-like					510:523	GM1-/GD1a-like	510:523	GM1-/GD1a-like	510:523	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	29	theme	ganglioside-core	468:483	arg1	tetra-/pentasaccharides					485:507	ganglioside-core tetra-/pentasaccharides	468:507	ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like)	468:524	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	29	theme	ganglioside-core	468:483	arg1	monosaccharides					393:407	eight or nine monosaccharides	379:407	eight or nine monosaccharides	379:407	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	1	30	theme	preceding	307:315	arg1	infection					317:325	a preceding infection	305:325	a preceding infection	305:325	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	2	31	theme	3-deoxy-d-manno-2-octulosonic	584:612	arg1	acid					614:617	3-deoxy-d-manno-2-octulosonic acid	584:617	3-deoxy-d-manno-2-octulosonic acid (KDO) residues	584:632	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	31	theme	3-deoxy-d-manno-2-octulosonic	584:612	arg1	KDO					620:622	KDO	620:622	KDO	620:622	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	5	32	theme	stereocontrolled	983:998	arg1	constructions					1011:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	4	33	theme	obtainable	725:734	arg1	l-glycero-d-manno-heptose					736:760	Less obtainable l-glycero-d-manno-heptose and KDO residues	720:777	l-glycero-d-manno-heptose	736:760	Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively.					
30351481	0	34	theme	Oligosaccharides	22:37	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.	0:133	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	0	35	from	jejuni	82:87	arg1	Lipooligosaccharides					42:61	Lipooligosaccharides	42:61	Lipooligosaccharides from Campylobacter jejuni	42:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	0	35	from	jejuni	82:87	arg1	Oligosaccharides					22:37	the Core Oligosaccharides	13:37	the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni	13:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	1	36	located	found	234:238	arg2	LOSs					228:231	LOSs	228:231	LOSs	228:231	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	1	36	located	found	234:238	arg1	jejuni					257:262	Campylobacter jejuni	243:262	Campylobacter jejuni	243:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	1	36	located	found	234:238	arg2	lipooligosaccharides					206:225	lipooligosaccharides	206:225	lipooligosaccharides (LOSs) found in Campylobacter jejuni	206:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	5	37	theme	α-KDO	937:941	arg1	glycoside					943:951	α-KDO glycoside	937:951	α-KDO glycoside	937:951	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	0	38	theme	Lipooligosaccharides	42:61	arg1	Oligosaccharides					22:37	the Core Oligosaccharides	13:37	the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni	13:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	6	39	theme	convergent	1207:1216	arg1	route					1228:1232	a convergent synthetic route	1205:1232	a convergent synthetic route	1205:1232	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	3	40	theme	synthetic	641:649	arg1	fragments					651:659	These synthetic fragments	635:659	These synthetic fragments	635:659	These synthetic fragments were obtained from commercially available monosaccharides.					
30351481	2	41	theme	target	346:351	arg1	LOS					353:355	The target LOS	342:355	The target LOS	342:355	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	6	42	theme	GD1a-core	1349:1357	arg1	sequences					1359:1367	GD1a-core sequences	1349:1367	GD1a-core sequences	1349:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	6	43	theme	LOS	1301:1303	arg1	glycans					1310:1316	structurally challenging LOS core glycans	1276:1316	structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1276:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	6	44	contain	containing	1318:1327	arg2	GM1					1341:1343	ganglioside GM1	1329:1343	ganglioside GM1	1329:1343	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	6	44	contain	containing	1318:1327	arg1	glycans					1310:1316	structurally challenging LOS core glycans	1276:1316	structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1276:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	6	44	contain	containing	1318:1327	arg2	sequences					1359:1367	GD1a-core sequences	1349:1367	GD1a-core sequences	1349:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	6	45	theme	first	1257:1261	arg1	synthesis					1263:1271	the first synthesis	1253:1271	the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences	1253:1367	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	3	46	theme	available	693:701	arg1	monosaccharides					703:717	commercially available monosaccharides	680:717	commercially available monosaccharides	680:717	These synthetic fragments were obtained from commercially available monosaccharides.					
30351481	1	47	theme	chemical	139:146	arg1	synthesis					148:156	The chemical synthesis	135:156	The chemical synthesis	135:156	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	6	48	theme	synthetic	1163:1171	arg1	blocks					1173:1178	All synthetic blocks	1159:1178	All synthetic blocks	1159:1178	All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.					
30351481	2	49	theme	l-glycero-d-manno-heptose-containing	527:562	arg1	monosaccharides					393:407	eight or nine monosaccharides	379:407	eight or nine monosaccharides	379:407	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	2	49	theme	l-glycero-d-manno-heptose-containing	527:562	arg1	trisaccharides					564:577	l-glycero-d-manno-heptose-containing trisaccharides	527:577	l-glycero-d-manno-heptose-containing trisaccharides	527:577	The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues.					
30351481	5	50	theme	α-directing	1138:1148	arg1	reagent					1150:1156	a suitable α-directing reagent	1127:1156	a suitable α-directing reagent	1127:1156	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	50	theme	α-directing	1138:1148	arg1	derivative					1061:1070	a 2,3-ene derivative	1051:1070	a 2,3-ene derivative of KDO	1051:1077	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	51	theme	glycosidic	1000:1009	arg1	constructions					1011:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	1	52	theme	Campylobacter	243:255	arg1	jejuni					257:262	Campylobacter jejuni	243:262	Campylobacter jejuni	243:262	The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described.					
30351481	0	53	theme	Campylobacter	68:80	arg1	jejuni					82:87	Campylobacter jejuni	68:87	Campylobacter jejuni	68:87	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	0	54	dep	Synthesis	0:8	arg1	Cause					101:105	A Putative Cause	90:105	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.	0:133	Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome.					
30351481	5	55	theme	constructions	1011:1023	arg1	one					957:959	one	957:959	one	957:959	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	55	theme	constructions	1011:1023	arg1	constructions					1011:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	the most difficult stereocontrolled glycosidic constructions	964:1023	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30351481	5	55	theme	constructions	1011:1023	arg1	synthesis					924:932	The synthesis	920:932	The synthesis of α-KDO glycoside	920:951	The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable α-directing reagent.					
30322851	13	0	theme	subsp	2270:2274	arg1	specificities					2237:2249	the relaxed specificities	2225:2249	the relaxed specificities of the N. elongata subsp	2225:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	2	1	theme	broad-spectrum	370:383	arg1	systems					422:428	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	6	2	theme	acid	957:960	arg1	biosynthesis					962:973	UDP-di-N-acetyl hexuronic acid biosynthesis	931:973	UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes	931:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	2	3	link	O-linked	385:392	arg1	Pgl					417:419	Pgl	417:419	Pgl	417:419	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	2	3	link	O-linked	385:392	arg1	glycosylation					402:414	O-linked protein glycosylation	385:414	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	13	4	theme	acid	2185:2188	arg1	utilization					2143:2153	the utilization	2139:2153	the utilization of UDP-di-N-acetyl-glucuronic acid	2139:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	6	5	theme	UDP-di-N-acetyl	931:945	arg1	biosynthesis					962:973	UDP-di-N-acetyl hexuronic acid biosynthesis	931:973	UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes	931:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	9	6	theme	conservative	1258:1269	arg1	nature					1271:1276	the conservative nature	1254:1276	the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1254:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	13	7	from	properties	2203:2212	arg1	utilization					2143:2153	the utilization	2139:2153	the utilization of UDP-di-N-acetyl-glucuronic acid	2139:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	6	8	located	found	975:979	arg2	biosynthesis					962:973	UDP-di-N-acetyl hexuronic acid biosynthesis	931:973	UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes	931:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	6	8	located	found	975:979	arg1	microbes					990:997	other microbes	984:997	other microbes	984:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	0	9	theme	Glycosylation	130:142	arg1	System					144:149	the O-Linked Protein Glycosylation System	109:149	the O-Linked Protein Glycosylation System of Neisseria elongata subsp	109:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	10	theme	UDP-di-N-acetyl	1291:1305	arg1	biosynthesis					1322:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	10	11	theme	surface	1782:1788	arg1	expression					1805:1814	microbial surface glycoconjugate expression	1772:1814	microbial surface glycoconjugate expression	1772:1814	Knowledge of how these systems relate structurally, biochemically, and evolutionarily to one another and to others associated with microbial surface glycoconjugate expression is still incomplete.					
30322851	10	12	dep	one	1730:1732	arg1	another					1734:1740	another	1734:1740	another	1734:1740	Knowledge of how these systems relate structurally, biochemically, and evolutionarily to one another and to others associated with microbial surface glycoconjugate expression is still incomplete.					
30322851	9	13	theme	acid	1317:1320	arg1	biosynthesis					1322:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	0	14	theme	elongata	164:171	arg1	subsp					173:177	Neisseria elongata subsp	154:177	Neisseria elongata subsp	154:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	5	15	from	end	870:872	arg1	acid					821:824	di-N-acetyl hexuronic acid	799:824	di-N-acetyl hexuronic acid	799:824	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	15	from	end	870:872	arg1	N-acetylhexosamine					832:849	a N-acetylhexosamine	830:849	a N-acetylhexosamine at the nonreducing end	830:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	13	16	from	utilization	2143:2153	arg1	degree					2089:2094	a significant degree	2075:2094	a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid	2075:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	13	16	from	utilization	2143:2153	arg1	properties					2203:2212	singular properties	2194:2212	singular properties related to the relaxed specificities of the N. elongata subsp	2194:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	6	17	theme	other	984:988	arg1	microbes					990:997	other microbes	984:997	other microbes	984:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	13	18	theme	intra-	2099:2104	arg1	conservation					2123:2134	intra- and transkingdom conservation	2099:2134	intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid	2099:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	9	19	theme	glycosylation	1343:1355	arg1	phenotypes					1357:1366	mutant glycosylation phenotypes	1336:1366	mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1336:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	6	20	theme	elongata	1068:1075	arg1	subsp					1077:1081	the N. elongata subsp	1061:1081	the N. elongata subsp	1061:1081	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	13	21	theme	transkingdom	2110:2121	arg1	conservation					2123:2134	intra- and transkingdom conservation	2099:2134	intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid	2099:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	11	22	theme	glycosylation	1912:1924	arg1	mutants					1926:1932	protein glycosylation mutants	1904:1932	protein glycosylation mutants of N. elongata subsp	1904:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	0	23	theme	Glycoform	63:71	arg1	Structure					73:81	Mature Glycoform Structure	56:81	Mature Glycoform Structure	56:81	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	5	24	theme	di-N-acetyl	799:809	arg1	acid					821:824	di-N-acetyl hexuronic acid	799:824	di-N-acetyl hexuronic acid	799:824	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	25	theme	unique	594:599	arg1	glycoform					617:625	a unique tetrasaccharide glycoform	592:625	a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end	592:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	25	theme	unique	594:599	arg1	glycolytica					579:589	glycolytica	579:589	glycolytica	579:589	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	11	26	theme	elongata	1940:1947	arg1	subsp					1949:1953	N. elongata subsp	1937:1953	N. elongata subsp	1937:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	5	27	theme	fragmentation	752:764	arg1	profile					766:772	spectrometric fragmentation profile	738:772	spectrometric fragmentation profile	738:772	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	13	28	from	degree	2089:2094	arg1	utilization					2143:2153	the utilization	2139:2153	the utilization of UDP-di-N-acetyl-glucuronic acid	2139:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	6	29	theme	related	1031:1037	arg1	components					1047:1056	related pathway components	1031:1056	related pathway components in the N. elongata subsp	1031:1081	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	0	30	theme	O-Linked	113:120	arg1	Glycosylation					130:142	O-Linked Protein Glycosylation	113:142	the O-Linked Protein Glycosylation System of Neisseria elongata subsp	109:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	13	31	theme	elongata	2261:2268	arg1	subsp					2270:2274	the N. elongata subsp	2254:2274	the N. elongata subsp	2254:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	0	32	theme	Sugar	41:45	arg1	Synthesis					10:18	Disrupted Synthesis	0:18	Disrupted Synthesis of a Di-N-acetylated Sugar	0:45	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	33	dep	unique	1375:1380	arg1	relaxed					1383:1389	relaxed	1383:1389	relaxed	1383:1389	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	3	34	theme	related	449:455	arg1	systems					461:467	related Pgl systems	449:467	related Pgl systems in other species in the genus	449:497	The potential for related Pgl systems in other species in the genus, however, remains to be determined.					
30322851	0	35	theme	Disrupted	0:8	arg1	Synthesis					10:18	Disrupted Synthesis	0:18	Disrupted Synthesis of a Di-N-acetylated Sugar	0:45	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	5	36	theme	nonreducing	858:868	arg1	end					870:872	the nonreducing end	854:872	the nonreducing end	854:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	6	37	theme	established	904:914	arg1	mechanisms					916:925	established mechanisms	904:925	established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes	904:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	4	38	theme	elongata	563:570	arg1	subsp					572:576	Neisseria elongata subsp	553:576	Neisseria elongata subsp	553:576	Using a strain of Neisseria elongata subsp.					
30322851	2	39	theme	genus	197:201	arg1	Neisseria					203:211	The genus Neisseria	193:211	The genus Neisseria	193:211	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	3	40	theme	other	472:476	arg1	species					478:484	other species	472:484	other species in the genus	472:497	The potential for related Pgl systems in other species in the genus, however, remains to be determined.					
30322851	0	41	theme	Di-N-acetylated	25:39	arg1	Sugar					41:45	a Di-N-acetylated Sugar	23:45	a Di-N-acetylated Sugar	23:45	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	42	theme	pathway	1479:1485	arg1	UDP-sugars					1500:1509	pathway intermediate UDP-sugars	1479:1509	pathway intermediate UDP-sugars	1479:1509	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	11	43	theme	reverse	1853:1859	arg1	efforts					1869:1875	reverse genetic efforts	1853:1875	reverse genetic efforts	1853:1875	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	3	44	from	species	478:484	arg1	genus					493:497	the genus	489:497	the genus	489:497	The potential for related Pgl systems in other species in the genus, however, remains to be determined.					
30322851	8	45	theme	genes	1147:1151	arg1	identification					1124:1137	the identification	1120:1137	the identification of such genes	1120:1151	Here, we detail the identification of such genes and the ensuing glycosylation phenotypes engendered by their inactivation.					
30322851	8	45	theme	genes	1147:1151	arg1	phenotypes					1183:1192	the ensuing glycosylation phenotypes	1157:1192	the ensuing glycosylation phenotypes engendered by their inactivation	1157:1225	Here, we detail the identification of such genes and the ensuing glycosylation phenotypes engendered by their inactivation.					
30322851	11	46	theme	protein	1904:1910	arg1	mutants					1926:1932	protein glycosylation mutants	1904:1932	protein glycosylation mutants of N. elongata subsp	1904:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	9	47	theme	mature	1516:1521	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	12	48	theme	UDP-sugar	2032:2040	arg1	precursor					2042:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	glycolytica that define the biosynthesis of a conserved but relatively rare UDP-sugar precursor.					
30322851	2	49	theme	glycosylation	402:414	arg1	systems					422:428	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	9	50	theme	Broad-spectrum	1545:1558	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	8	51	theme	glycosylation	1169:1181	arg1	phenotypes					1183:1192	the ensuing glycosylation phenotypes	1157:1192	the ensuing glycosylation phenotypes engendered by their inactivation	1157:1225	Here, we detail the identification of such genes and the ensuing glycosylation phenotypes engendered by their inactivation.					
30322851	5	52	gly	glycoform	617:625	arg1	tetrasaccharide					601:615	a unique tetrasaccharide glycoform	592:625	a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end	592:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	2	53	theme	O-linked	385:392	arg1	Pgl					417:419	Pgl	417:419	Pgl	417:419	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	2	53	theme	O-linked	385:392	arg1	glycosylation					402:414	O-linked protein glycosylation	385:414	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	9	54	theme	glycosylation	1568:1580	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	9	55	dep	phenotypes	1357:1366	arg1	reveal					1368:1373	reveal	1368:1373	reveal unique, relaxed specificities of the glycosyltransferases	1368:1431	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	9	55	dep	phenotypes	1357:1366	arg1	oligosaccharyltransferases					1437:1462	oligosaccharyltransferases	1437:1462	oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1437:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	5	56	theme	first	685:689	arg1	sugars					695:700	the first two sugars	681:700	the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end	681:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	13	57	theme	UDP-di-N-acetyl-glucuronic	2158:2183	arg1	acid					2185:2188	UDP-di-N-acetyl-glucuronic acid	2158:2188	UDP-di-N-acetyl-glucuronic acid	2158:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	9	58	theme	Pgl	1583:1585	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	6	59	theme	hexuronic	947:955	arg1	biosynthesis					962:973	UDP-di-N-acetyl hexuronic acid biosynthesis	931:973	UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes	931:997	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	13	60	theme	relaxed	2229:2235	arg1	specificities					2237:2249	the relaxed specificities	2225:2249	the relaxed specificities of the N. elongata subsp	2225:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	7	61	dep	genome	1096:1101	arg1	glycolytica					1084:1094	glycolytica	1084:1094	glycolytica	1084:1094	glycolytica genome.					
30322851	2	62	theme	Neisseria	336:344	arg1	lactamica					346:354	Neisseria lactamica	336:354	Neisseria lactamica	336:354	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	13	63	theme	conservation	2123:2134	arg1	degree					2089:2094	a significant degree	2075:2094	a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid	2075:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	13	63	theme	conservation	2123:2134	arg1	properties					2203:2212	singular properties	2194:2212	singular properties related to the relaxed specificities of the N. elongata subsp	2194:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	2	64	dep	disease	276:282	arg1	lactamica					346:354	Neisseria lactamica	336:354	Neisseria lactamica	336:354	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	2	64	dep	disease	276:282	arg1	gonorrhoeae					295:305	Neisseria gonorrhoeae	285:305	Neisseria gonorrhoeae	285:305	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	2	64	dep	disease	276:282	arg1	meningitidis					318:329	Neisseria meningitidis	308:329	Neisseria meningitidis	308:329	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	5	65	theme	rare	716:719	arg1	sugar					721:725	a rare sugar	714:725	a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end	714:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	9	66	theme	microbial	1281:1289	arg1	biosynthesis					1322:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	10	67	theme	glycoconjugate	1790:1803	arg1	expression					1805:1814	microbial surface glycoconjugate expression	1772:1814	microbial surface glycoconjugate expression	1772:1814	Knowledge of how these systems relate structurally, biochemically, and evolutionarily to one another and to others associated with microbial surface glycoconjugate expression is still incomplete.					
30322851	0	68	theme	Neisseria	154:162	arg1	subsp					173:177	Neisseria elongata subsp	154:177	Neisseria elongata subsp	154:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	69	theme	hexuronic	1307:1315	arg1	biosynthesis					1322:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1281:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	2	70	theme	human	259:263	arg1	health					265:270	human health	259:270	human health	259:270	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	10	71	theme	microbial	1772:1780	arg1	expression					1805:1814	microbial surface glycoconjugate expression	1772:1814	microbial surface glycoconjugate expression	1772:1814	Knowledge of how these systems relate structurally, biochemically, and evolutionarily to one another and to others associated with microbial surface glycoconjugate expression is still incomplete.					
30322851	0	72	theme	subsp	173:177	arg1	System					144:149	the O-Linked Protein Glycosylation System	109:149	the O-Linked Protein Glycosylation System of Neisseria elongata subsp	109:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	73	theme	biosynthesis	1322:1333	arg1	nature					1271:1276	the conservative nature	1254:1276	the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis	1254:1333	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	2	74	theme	importance	245:254	arg1	species					234:240	three major species	222:240	three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems	222:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	12	75	theme	precursor	2042:2050	arg1	biosynthesis					1984:1995	the biosynthesis	1980:1995	the biosynthesis of a conserved but relatively rare UDP-sugar precursor	1980:2050	glycolytica that define the biosynthesis of a conserved but relatively rare UDP-sugar precursor.					
30322851	2	76	theme	major	228:232	arg1	species					234:240	three major species	222:240	three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems	222:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	9	77	theme	mutant	1336:1341	arg1	phenotypes					1357:1366	mutant glycosylation phenotypes	1336:1366	mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1336:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	13	78	from	conservation	2123:2134	arg1	utilization					2143:2153	the utilization	2139:2153	the utilization of UDP-di-N-acetyl-glucuronic acid	2139:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	11	79	theme	N.	1937:1938	arg1	subsp					1949:1953	N. elongata subsp	1937:1953	N. elongata subsp	1937:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	6	80	theme	N.	1065:1066	arg1	subsp					1077:1081	the N. elongata subsp	1061:1081	the N. elongata subsp	1061:1081	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	5	81	with	consistent	783:792	arg1	acid					821:824	di-N-acetyl hexuronic acid	799:824	di-N-acetyl hexuronic acid	799:824	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	81	with	consistent	783:792	arg1	N-acetylhexosamine					832:849	a N-acetylhexosamine	830:849	a N-acetylhexosamine at the nonreducing end	830:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	11	82	theme	mutants	1926:1932	arg1	characterization					1884:1899	characterization	1884:1899	characterization of protein glycosylation mutants of N. elongata subsp	1884:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	9	83	theme	unique	1375:1380	arg1	specificities					1391:1403	unique, relaxed specificities	1375:1403	unique, relaxed specificities of the glycosyltransferases	1375:1431	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	13	84	theme	significant	2077:2087	arg1	degree					2089:2094	a significant degree	2075:2094	a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid	2075:2188	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	5	85	theme	spectrometric	738:750	arg1	profile					766:772	spectrometric fragmentation profile	738:772	spectrometric fragmentation profile	738:772	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	86	theme	tetrasaccharide	601:615	arg1	glycoform					617:625	a unique tetrasaccharide glycoform	592:625	a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end	592:872	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	5	86	theme	tetrasaccharide	601:615	arg1	glycolytica					579:589	glycolytica	579:589	glycolytica	579:589	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	6	87	theme	pathway	1039:1045	arg1	components					1047:1056	related pathway components	1031:1056	related pathway components in the N. elongata subsp	1031:1081	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	11	88	theme	subsp	1949:1953	arg1	mutants					1926:1932	protein glycosylation mutants	1904:1932	protein glycosylation mutants of N. elongata subsp	1904:1953	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	0	89	theme	Protein	122:128	arg1	Glycosylation					130:142	O-Linked Protein Glycosylation	113:142	the O-Linked Protein Glycosylation System of Neisseria elongata subsp	109:177	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	13	90	theme	N.	2258:2259	arg1	subsp					2270:2274	the N. elongata subsp	2254:2274	the N. elongata subsp	2254:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	9	91	theme	glycosyltransferases	1412:1431	arg1	specificities					1391:1403	unique, relaxed specificities	1375:1403	unique, relaxed specificities of the glycosyltransferases	1375:1431	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	4	92	theme	Neisseria	553:561	arg1	subsp					572:576	Neisseria elongata subsp	553:576	Neisseria elongata subsp	553:576	Using a strain of Neisseria elongata subsp.					
30322851	6	93	from	components	1047:1056	arg1	subsp					1077:1081	the N. elongata subsp	1061:1081	the N. elongata subsp	1061:1081	Based on established mechanisms for UDP-di-N-acetyl hexuronic acid biosynthesis found in other microbes, we searched for genes encoding related pathway components in the N. elongata subsp.					
30322851	3	94	theme	Pgl	457:459	arg1	systems					461:467	related Pgl systems	449:467	related Pgl systems in other species in the genus	449:497	The potential for related Pgl systems in other species in the genus, however, remains to be determined.					
30322851	4	95	theme	subsp	572:576	arg1	strain					543:548	a strain	541:548	a strain of Neisseria elongata subsp	541:576	Using a strain of Neisseria elongata subsp.					
30322851	11	96	theme	genetic	1861:1867	arg1	efforts					1869:1875	reverse genetic efforts	1853:1875	reverse genetic efforts	1853:1875	Here, we detail reverse genetic efforts toward characterization of protein glycosylation mutants of N. elongata subsp.					
30322851	5	97	theme	hexuronic	811:819	arg1	acid					821:824	di-N-acetyl hexuronic acid	799:824	di-N-acetyl hexuronic acid	799:824	glycolytica, a unique tetrasaccharide glycoform consisting of di-N-acetylbacillosamine and glucose as the first two sugars followed by a rare sugar whose mass spectrometric fragmentation profile was most consistent with di-N-acetyl hexuronic acid and a N-acetylhexosamine at the nonreducing end has been identified.					
30322851	8	98	theme	such	1142:1145	arg1	genes					1147:1151	such genes	1142:1151	such genes	1142:1151	Here, we detail the identification of such genes and the ensuing glycosylation phenotypes engendered by their inactivation.					
30322851	0	99	theme	Mature	56:61	arg1	Structure					73:81	Mature Glycoform Structure	56:81	Mature Glycoform Structure	56:81	Disrupted Synthesis of a Di-N-acetylated Sugar Perturbs Mature Glycoform Structure and Microheterogeneity in the O-Linked Protein Glycosylation System of Neisseria elongata subsp.					
30322851	9	100	theme	intermediate	1487:1498	arg1	UDP-sugars					1500:1509	pathway intermediate UDP-sugars	1479:1509	pathway intermediate UDP-sugars	1479:1509	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	12	101	theme	rare	2027:2030	arg1	precursor					2042:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	glycolytica that define the biosynthesis of a conserved but relatively rare UDP-sugar precursor.					
30322851	8	102	theme	ensuing	1161:1167	arg1	phenotypes					1183:1192	the ensuing glycosylation phenotypes	1157:1192	the ensuing glycosylation phenotypes engendered by their inactivation	1157:1225	Here, we detail the identification of such genes and the ensuing glycosylation phenotypes engendered by their inactivation.					
30322851	12	103	theme	conserved	2002:2010	arg1	precursor					2042:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	a conserved but relatively rare UDP-sugar precursor	2000:2050	glycolytica that define the biosynthesis of a conserved but relatively rare UDP-sugar precursor.					
30322851	13	104	theme	related	2214:2220	arg1	properties					2203:2212	singular properties	2194:2212	singular properties related to the relaxed specificities of the N. elongata subsp	2194:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	13	105	theme	singular	2194:2201	arg1	properties					2203:2212	singular properties	2194:2212	singular properties related to the relaxed specificities of the N. elongata subsp	2194:2274	The results show both a significant degree of intra- and transkingdom conservation in the utilization of UDP-di-N-acetyl-glucuronic acid and singular properties related to the relaxed specificities of the N. elongata subsp.					
30322851	9	106	theme	glycoforms.IMPORTANCE	1523:1543	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30322851	3	107	from	systems	461:467	arg1	species					478:484	other species	472:484	other species in the genus	472:497	The potential for related Pgl systems in other species in the genus, however, remains to be determined.					
30322851	2	108	theme	protein	394:400	arg1	Pgl					417:419	Pgl	417:419	Pgl	417:419	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	2	108	theme	protein	394:400	arg1	glycosylation					402:414	O-linked protein glycosylation	385:414	broad-spectrum O-linked protein glycosylation (Pgl) systems	370:428	The genus Neisseria includes three major species of importance to human health and disease (Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica) that express broad-spectrum O-linked protein glycosylation (Pgl) systems.					
30322851	9	109	theme	protein	1560:1566	arg1	systems					1588:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems	1516:1594	While the findings extend the conservative nature of microbial UDP-di-N-acetyl hexuronic acid biosynthesis, mutant glycosylation phenotypes reveal unique, relaxed specificities of the glycosyltransferases and oligosaccharyltransferases to incorporate pathway intermediate UDP-sugars into mature glycoforms.IMPORTANCE Broad-spectrum protein glycosylation (Pgl) systems are well recognized in bacteria and archaea.					
30315110	4	0	theme	bacterial	601:609	arg1	pathogen					611:618	The opportunistic bacterial pathogen	583:618	The opportunistic bacterial pathogen Vibrio vulnificus	583:636	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	2	1	theme	N-acetylneuraminic	357:374	arg1	which					350:354	which	350:354	which	350:354	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	1	theme	N-acetylneuraminic	357:374	arg1	acid					376:379	N-acetylneuraminic acid	357:379	N-acetylneuraminic acid (sialic acid)	357:393	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	1	theme	N-acetylneuraminic	357:374	arg1	acid					389:392	sialic acid	382:392	sialic acid	382:392	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	1	theme	N-acetylneuraminic	357:374	arg1	one					343:345	one	343:345	one	343:345	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	10	2	dep	V.	1736:1737	arg1	vulnificus					1739:1748	vulnificus	1739:1748	vulnificus	1739:1748	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	3	3	theme	host-pathogen	545:557	arg1	mechanisms					571:580	host-pathogen recognition mechanisms	545:580	host-pathogen recognition mechanisms	545:580	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	8	4	theme	bacterial	1310:1318	arg1	integrity					1335:1343	bacterial outer-membrane integrity	1310:1343	bacterial outer-membrane integrity	1310:1343	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	3	5	theme	molecules	530:538	arg1	identities					510:519	the identities	506:519	the identities of these molecules	506:538	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	5	6	theme	vulnificus	863:872	arg1	strain					874:879	a clinical V. vulnificus strain CMCP6	849:885	a clinical V. vulnificus strain CMCP6	849:885	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	7	7	theme	In	1135:1136	arg1	analysis					1145:1152	In silico analysis	1135:1152	In silico analysis	1135:1152	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	8	8	from	defects	1519:1525	arg1	formation					1538:1546	biofilm formation	1530:1546	biofilm formation	1530:1546	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	1	9	theme	α-keto	186:191	arg1	carbohydrates					198:210	α-keto acid carbohydrates	186:210	α-keto acid carbohydrates present across all branches of life	186:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	4	10	theme	NulO	662:665	arg1	biosynthesis					667:678	NulO biosynthesis	662:678	NulO biosynthesis	662:678	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	2	11	theme	related	302:308	arg1	isomers					331:337	several related prokaryotic-specific isomers	294:337	several related prokaryotic-specific isomers	294:337	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	1	12	theme	carbohydrates	198:210	arg1	acids					148:152	Nonulosonic acids	136:152	Nonulosonic acids (NulOs)	136:160	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	1	12	theme	carbohydrates	198:210	arg1	family					176:181	a diverse family	166:181	a diverse family of α-keto acid carbohydrates present across all branches of life	166:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	6	13	theme	modified	989:996	arg1	acid					1011:1014	modified legionaminic acid	989:1014	modified legionaminic acid	989:1014	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	7	14	dep	In	1135:1136	arg1	silico					1138:1143	silico	1138:1143	silico	1138:1143	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	10	15	theme	CMCP6	1925:1929	arg1	survivability					1875:1887	survivability	1875:1887	survivability	1875:1887	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	10	15	theme	CMCP6	1925:1929	arg1	pathogenicity					1894:1906	pathogenicity	1894:1906	pathogenicity	1894:1906	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	10	15	theme	CMCP6	1925:1929	arg1	physiology					1863:1872	physiology	1863:1872	physiology	1863:1872	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	5	16	theme	clinical	851:858	arg1	strain					874:879	a clinical V. vulnificus strain CMCP6	849:885	a clinical V. vulnificus strain CMCP6	849:885	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	4	17	theme	vulnificus	729:738	arg1	glycan					745:750	the V. vulnificus NulO glycan	722:750	the V. vulnificus NulO glycan	722:750	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	10	18	theme	V.	1911:1912	arg1	CMCP6					1925:1929	V. vulnificus CMCP6	1911:1929	V. vulnificus CMCP6	1911:1929	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	9	19	theme	Artemia	1577:1583	arg1	franciscana					1585:1595	Artemia franciscana	1577:1595	Artemia franciscana	1577:1595	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	9	19	theme	Artemia	1577:1583	arg1	model					1570:1574	the crustacean model	1555:1574	the crustacean model	1555:1574	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	6	20	theme	CMCP6	959:963	arg1	strain					965:970	The CMCP6 strain	955:970	The CMCP6 strain	955:970	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	7	21	attach	present	1244:1250	arg2	biosynthesis					1182:1193	Leg5Ac7AcAla biosynthesis	1169:1193	Leg5Ac7AcAla biosynthesis	1169:1193	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	7	21	attach	present	1244:1250	arg1	species					1273:1279	several bacterial species	1255:1279	several bacterial species	1255:1279	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	4	22	theme	glycan	745:750	arg1	functions					709:717	functions	709:717	functions	709:717	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	4	22	theme	glycan	745:750	arg1	structures					694:703	structures	694:703	structures	694:703	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	10	23	dep	physiology	1863:1872	arg1	the					1859:1861	the	1859:1861	the	1859:1861	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	0	24	theme	acid	70:73	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of a modified legionaminic acid	0:73	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	4	25	contain	possesses	638:646	arg1	pathogen					611:618	The opportunistic bacterial pathogen	583:618	The opportunistic bacterial pathogen Vibrio vulnificus	583:636	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	4	25	contain	possesses	638:646	arg2	genes					652:656	the genes	648:656	the genes for NulO biosynthesis	648:678	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	5	26	theme	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic	890:932	arg1	acid					934:937	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid	890:937	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla)	890:952	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	5	26	theme	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic	890:932	arg1	Leg5Ac7AcAla					940:951	Leg5Ac7AcAla	940:951	Leg5Ac7AcAla	940:951	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	5	26	theme	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic	890:932	arg1	NulO					832:835	the major NulO	822:835	the major NulO produced by a clinical V. vulnificus strain CMCP6	822:885	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	9	27	theme	Leg5Ac7AcAla-deficient	1618:1639	arg1	bacteria					1641:1648	Leg5Ac7AcAla-deficient bacteria	1618:1648	Leg5Ac7AcAla-deficient bacteria	1618:1648	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	6	28	theme	vulnificus	1028:1037	arg1	strain					1039:1044	V. vulnificus strain YJ016	1025:1050	V. vulnificus strain YJ016	1025:1050	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	7	29	theme	bacterial	1263:1271	arg1	species					1273:1279	several bacterial species	1255:1279	several bacterial species	1255:1279	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	8	30	theme	membrane	1438:1445	arg1	permeability					1447:1458	increased membrane permeability	1428:1458	increased membrane permeability	1428:1458	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	0	31	theme	bacterial	106:114	arg1	lipopolysaccharide					116:133	a bacterial lipopolysaccharide	104:133	a bacterial lipopolysaccharide	104:133	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	0	32	gly	glycosylation	87:99	arg1	lipopolysaccharide					116:133	a bacterial lipopolysaccharide	104:133	a bacterial lipopolysaccharide	104:133	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	6	33	theme	N-acetyl-d-alanyl	1103:1119	arg1	modification					1121:1132	the N-acetyl-d-alanyl modification	1099:1132	the N-acetyl-d-alanyl modification	1099:1132	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	0	34	theme	Structural	0:9	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of a modified legionaminic acid	0:73	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	10	35	theme	modified	1794:1801	arg1	Leg5Ac7AcAla					1821:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	0	36	theme	functional	15:24	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of a modified legionaminic acid	0:73	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	3	37	theme	NulO	454:457	arg1	carbohydrates					459:471	various NulO carbohydrates	446:471	various NulO carbohydrates on lipopolysaccharide (LPS)	446:499	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	5	38	theme	V.	860:861	arg1	strain					874:879	a clinical V. vulnificus strain CMCP6	849:885	a clinical V. vulnificus strain CMCP6	849:885	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	8	39	theme	antimicrobial	1491:1503	arg1	peptides					1505:1512	antimicrobial peptides	1491:1512	antimicrobial peptides	1491:1512	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	3	40	from	carbohydrates	459:471	arg1	LPS					496:498	LPS	496:498	LPS	496:498	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	3	40	from	carbohydrates	459:471	arg1	lipopolysaccharide					476:493	lipopolysaccharide	476:493	lipopolysaccharide (LPS)	476:499	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	0	41	theme	modified	48:55	arg1	acid					70:73	a modified legionaminic acid	46:73	a modified legionaminic acid	46:73	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	7	42	located	present	1244:1250	arg2	biosynthesis					1182:1193	Leg5Ac7AcAla biosynthesis	1169:1193	Leg5Ac7AcAla biosynthesis	1169:1193	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	7	42	located	present	1244:1250	arg1	species					1273:1279	several bacterial species	1255:1279	several bacterial species	1255:1279	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	10	43	theme	acid	1816:1819	arg1	Leg5Ac7AcAla					1821:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	2	44	theme	sialic	382:387	arg1	acid					376:379	N-acetylneuraminic acid	357:379	N-acetylneuraminic acid (sialic acid)	357:393	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	44	theme	sialic	382:387	arg1	acid					389:392	sialic acid	382:392	sialic acid	382:392	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	9	45	contain	have	1650:1653	arg1	bacteria					1641:1648	Leg5Ac7AcAla-deficient bacteria	1618:1648	Leg5Ac7AcAla-deficient bacteria	1618:1648	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	9	45	contain	have	1650:1653	arg2	potential					1675:1683	decreased virulence potential	1655:1683	decreased virulence potential	1655:1683	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	7	46	theme	Leg5Ac7AcAla	1169:1180	arg1	biosynthesis					1182:1193	Leg5Ac7AcAla biosynthesis	1169:1193	Leg5Ac7AcAla biosynthesis	1169:1193	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	10	47	theme	critical	1842:1849	arg1	role					1851:1854	a critical role	1840:1854	a critical role	1840:1854	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	8	48	from	sensitivity	1461:1471	arg1	formation					1538:1546	biofilm formation	1530:1546	biofilm formation	1530:1546	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	4	49	theme	opportunistic	587:599	arg1	pathogen					611:618	The opportunistic bacterial pathogen	583:618	The opportunistic bacterial pathogen Vibrio vulnificus	583:636	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	2	50	theme	several	294:300	arg1	isomers					331:337	several related prokaryotic-specific isomers	294:337	several related prokaryotic-specific isomers	294:337	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	8	51	from	permeability	1447:1458	arg1	formation					1538:1546	biofilm formation	1530:1546	biofilm formation	1530:1546	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	1	52	theme	diverse	168:174	arg1	acids					148:152	Nonulosonic acids	136:152	Nonulosonic acids (NulOs)	136:160	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	1	52	theme	diverse	168:174	arg1	family					176:181	a diverse family	166:181	a diverse family of α-keto acid carbohydrates present across all branches of life	166:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	8	53	theme	outer-membrane	1320:1333	arg1	integrity					1335:1343	bacterial outer-membrane integrity	1310:1343	bacterial outer-membrane integrity	1310:1343	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	2	54	theme	prokaryotic-specific	310:329	arg1	isomers					331:337	several related prokaryotic-specific isomers	294:337	several related prokaryotic-specific isomers	294:337	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	1	55	theme	acid	193:196	arg1	carbohydrates					198:210	α-keto acid carbohydrates	186:210	α-keto acid carbohydrates present across all branches of life	186:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	6	56	theme	legionaminic	998:1009	arg1	acid					1011:1014	modified legionaminic acid	989:1014	modified legionaminic acid	989:1014	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	7	57	from	present	1244:1250	arg1	species					1273:1279	several bacterial species	1255:1279	several bacterial species	1255:1279	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	10	58	dep	V.	1911:1912	arg1	vulnificus					1914:1923	vulnificus	1914:1923	vulnificus	1914:1923	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	4	59	dep	pathogen	611:618	arg1	vulnificus					627:636	Vibrio vulnificus	620:636	The opportunistic bacterial pathogen Vibrio vulnificus	583:636	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	9	60	theme	crustacean	1559:1568	arg1	franciscana					1585:1595	Artemia franciscana	1577:1595	Artemia franciscana	1577:1595	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	9	60	theme	crustacean	1559:1568	arg1	model					1570:1574	the crustacean model	1555:1574	the crustacean model	1555:1574	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	6	61	dep	catabolize	978:987	arg1	whereas					1017:1023	whereas	1017:1023	whereas	1017:1023	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	8	62	theme	mutant	1349:1354	arg1	strains					1356:1362	mutant strains	1349:1362	mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS	1349:1421	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	10	63	theme	different	1726:1734	arg1	strains					1750:1756	different V. vulnificus strains	1726:1756	different V. vulnificus strains	1726:1756	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	7	64	from	species	1273:1279	arg1	present					1244:1250	present	1244:1250	present	1244:1250	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	8	65	theme	unable	1364:1369	arg1	strains					1356:1362	mutant strains	1349:1362	mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS	1349:1421	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	3	66	theme	recognition	559:569	arg1	mechanisms					571:580	host-pathogen recognition mechanisms	545:580	host-pathogen recognition mechanisms	545:580	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	4	67	theme	NulO	740:743	arg1	glycan					745:750	the V. vulnificus NulO glycan	722:750	the V. vulnificus NulO glycan	722:750	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	5	68	theme	genetic	771:777	arg1	approaches					792:801	genetic and chemical approaches	771:801	genetic and chemical approaches	771:801	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	1	69	theme	Nonulosonic	136:146	arg1	acids					148:152	Nonulosonic acids	136:152	Nonulosonic acids (NulOs)	136:160	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	1	69	theme	Nonulosonic	136:146	arg1	family					176:181	a diverse family	166:181	a diverse family of α-keto acid carbohydrates present across all branches of life	166:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	1	69	theme	Nonulosonic	136:146	arg1	NulOs					155:159	NulOs	155:159	NulOs	155:159	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	5	70	theme	chemical	783:790	arg1	approaches					792:801	genetic and chemical approaches	771:801	genetic and chemical approaches	771:801	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	8	71	theme	biofilm	1530:1536	arg1	formation					1538:1546	biofilm formation	1530:1546	biofilm formation	1530:1546	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	7	72	theme	several	1255:1261	arg1	species					1273:1279	several bacterial species	1255:1279	several bacterial species	1255:1279	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	6	73	theme	V.	1025:1026	arg1	strain					1039:1044	V. vulnificus strain YJ016	1025:1050	V. vulnificus strain YJ016	1025:1050	The CMCP6 strain could catabolize modified legionaminic acid, whereas V. vulnificus strain YJ016 produced but did not catabolize a NulO without the N-acetyl-d-alanyl modification.					
30315110	0	74	theme	lipopolysaccharide	116:133	arg1	glycosylation					87:99	glycosylation	87:99	glycosylation of a bacterial lipopolysaccharide	87:133	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	9	75	theme	decreased	1655:1663	arg1	potential					1675:1683	decreased virulence potential	1655:1683	decreased virulence potential	1655:1683	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	8	76	contain	have	1423:1426	arg2	defects					1519:1525	defects	1519:1525	defects in biofilm formation	1519:1546	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	8	76	contain	have	1423:1426	arg2	sensitivity					1461:1471	sensitivity	1461:1471	sensitivity to bile salts and antimicrobial peptides	1461:1512	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	8	76	contain	have	1423:1426	arg2	permeability					1447:1458	increased membrane permeability	1428:1458	increased membrane permeability	1428:1458	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	8	76	contain	have	1423:1426	arg1	strains					1356:1362	mutant strains	1349:1362	mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS	1349:1421	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	8	77	theme	increased	1428:1436	arg1	permeability					1447:1458	increased membrane permeability	1428:1458	increased membrane permeability	1428:1458	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	10	78	theme	multiple	1766:1773	arg1	NulOs					1775:1779	multiple NulOs	1766:1779	multiple NulOs	1766:1779	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	5	79	theme	major	826:830	arg1	acid					934:937	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid	890:937	5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla)	890:952	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	5	79	theme	major	826:830	arg1	NulO					832:835	the major NulO	822:835	the major NulO produced by a clinical V. vulnificus strain CMCP6	822:885	Using genetic and chemical approaches, we show here that the major NulO produced by a clinical V. vulnificus strain CMCP6 is 5-N-acetyl-7-N-acetyl-d-alanyl-legionaminic acid (Leg5Ac7AcAla).					
30315110	7	80	theme	noncanonical	1205:1216	arg1	pathway					1218:1224	a noncanonical pathway	1203:1224	a noncanonical pathway	1203:1224	In silico analysis suggested that Leg5Ac7AcAla biosynthesis follows a noncanonical pathway but appears to be present in several bacterial species.					
30315110	1	81	theme	present	212:218	arg1	carbohydrates					198:210	α-keto acid carbohydrates	186:210	α-keto acid carbohydrates present across all branches of life	186:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	10	82	theme	V.	1736:1737	arg1	strains					1750:1756	different V. vulnificus strains	1726:1756	different V. vulnificus strains	1726:1756	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	8	83	theme	bile	1476:1479	arg1	salts					1481:1485	bile salts	1476:1485	bile salts	1476:1485	Leg5Ac7AcAla contributed to bacterial outer-membrane integrity, as mutant strains unable to produce or incorporate Leg5Ac7AcAla into the LPS have increased membrane permeability, sensitivity to bile salts and antimicrobial peptides, and defects in biofilm formation.					
30315110	0	84	theme	legionaminic	57:68	arg1	acid					70:73	a modified legionaminic acid	46:73	a modified legionaminic acid	46:73	Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.					
30315110	2	85	theme	which	350:354	arg1	which					350:354	which	350:354	which	350:354	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	85	theme	which	350:354	arg1	acid					376:379	N-acetylneuraminic acid	357:379	N-acetylneuraminic acid (sialic acid)	357:393	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	2	85	theme	which	350:354	arg1	one					343:345	one	343:345	one	343:345	Bacteria biosynthesize NulOs among which are several related prokaryotic-specific isomers and one of which, N-acetylneuraminic acid (sialic acid), is common among all vertebrates.					
30315110	4	86	dep	structures	694:703	arg1	the					690:692	the	690:692	the	690:692	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
30315110	9	87	theme	virulence	1665:1673	arg1	potential					1675:1683	decreased virulence potential	1655:1683	decreased virulence potential	1655:1683	Using the crustacean model, Artemia franciscana, we demonstrate that Leg5Ac7AcAla-deficient bacteria have decreased virulence potential compared with WT.					
30315110	10	88	theme	legionaminic	1803:1814	arg1	Leg5Ac7AcAla					1821:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	the modified legionaminic acid Leg5Ac7AcAla	1790:1832	Our data indicate that different V. vulnificus strains produce multiple NulOs and that the modified legionaminic acid Leg5Ac7AcAla plays a critical role in the physiology, survivability, and pathogenicity of V. vulnificus CMCP6.					
30315110	3	89	theme	various	446:452	arg1	carbohydrates					459:471	various NulO carbohydrates	446:471	various NulO carbohydrates on lipopolysaccharide (LPS)	446:499	Bacteria display various NulO carbohydrates on lipopolysaccharide (LPS), and the identities of these molecules tune host-pathogen recognition mechanisms.					
30315110	1	90	theme	life	243:246	arg1	branches					231:238	all branches	227:238	all branches of life	227:246	Nonulosonic acids (NulOs) are a diverse family of α-keto acid carbohydrates present across all branches of life.					
30315110	4	91	theme	V.	726:727	arg1	glycan					745:750	the V. vulnificus NulO glycan	722:750	the V. vulnificus NulO glycan	722:750	The opportunistic bacterial pathogen Vibrio vulnificus possesses the genes for NulO biosynthesis; however, the structures and functions of the V. vulnificus NulO glycan are unknown.					
29730471	6	0	dep	decrease	1135:1142	arg1	i					1130:1130	i	1130:1130	i	1130:1130	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	1	theme	lectin-binding	1007:1020	arg1	profiles					1022:1029	lectin-binding profiles	1007:1029	lectin-binding profiles of the secretory cells of Brunner's glands in these two groups	1007:1092	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	2	link	α1,2-linked	1175:1185	arg1	fucose					1187:1192	α1,2-linked fucose	1175:1192	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	2	link	α1,2-linked	1175:1185	arg1	A					1199:1199	Con A	1195:1199	Con A	1195:1199	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	3	3	theme	tissue	597:602	arg1	fragments					604:612	Duodenal tissue fragments	588:612	Duodenal tissue fragments	588:612	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	1	4	theme	lectin	391:396	arg1	histochemistry					411:424	conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry	303:424	histochemistry	411:424	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	4	theme	lectin	391:396	arg1	lectins					402:408	15 lectins	399:408	15 lectins	399:408	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	3	5	theme	%	629:629	arg1	paraformaldehyde					666:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	6	6	dep	fucose	1187:1192	arg1	loss					1217:1220	a loss	1215:1220	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	9	7	theme	animals	1888:1894	arg1	function					1852:1859	gastrointestinal function	1835:1859	gastrointestinal function	1835:1859	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	9	7	theme	animals	1888:1894	arg1	status					1872:1877	health status	1865:1877	health status	1865:1877	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	1	8	theme	diamine	369:375	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	8	theme	diamine	369:375	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	9	theme	carbohydrate	164:175	arg1	composition					177:187	the carbohydrate composition	160:187	the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs	160:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	8	10	theme	mucins	1725:1730	arg1	composition					1710:1720	the glycan composition	1699:1720	the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs	1699:1789	These results indicate that probiotic supplementation affects the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs.					
29730471	7	11	theme	Pro	1498:1500	arg1	samples					1502:1508	No-Pro and Pro samples	1487:1508	No-Pro and Pro samples	1487:1508	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	6	12	theme	UEA	1202:1204	arg1	I					1206:1206	UEA I	1202:1206	UEA I	1202:1206	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	2	13	theme	basal	483:487	arg1	diet					489:492	a control basal diet	473:492	a control basal diet without the probiotic blend (No-Pro)	473:529	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	13	theme	basal	483:487	arg1	treatments					461:470	two dietary treatments	449:470	two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro)	449:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	3	14	theme	alcohol	712:718	arg1	series					720:725	a graded alcohol series	703:725	a graded alcohol series	703:725	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	1	15	theme	high	359:362	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	15	theme	high	359:362	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	9	16	theme	growth	1939:1944	arg1	performance					1946:1956	higher growth performance	1932:1956	higher growth performance	1932:1956	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	3	17	from	fixed	619:623	arg1	paraformaldehyde					666:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	9	18	theme	higher	1932:1937	arg1	performance					1946:1956	higher growth performance	1932:1956	higher growth performance	1932:1956	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	9	19	from	quality	1967:1973	arg1	group					1997:2001	the test probiotic group	1978:2001	the test probiotic group	1978:2001	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	6	20	from	types	1233:1237	arg1	O-glycans					1454:1462	O-glycans	1454:1462	O-glycans containing GalNAc HPA	1454:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	4	21	gly	glycoproteins	846:858	arg1	glycoproteins					846:858	neutral glycoproteins	838:858	neutral glycoproteins	838:858	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	2	22	theme	probiotic	506:514	arg1	No-Pro					523:528	No-Pro	523:528	No-Pro	523:528	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	22	theme	probiotic	506:514	arg1	blend					516:520	the probiotic blend	502:520	the probiotic blend (No-Pro)	502:529	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	23	theme	test	537:540	arg1	diet					542:545	a test diet	535:545	a test diet that included the probiotic blend (Pro)	535:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	23	theme	test	537:540	arg1	treatments					461:470	two dietary treatments	449:470	two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro)	449:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	6	24	theme	α1,2-linked	1175:1185	arg1	fucose					1187:1192	α1,2-linked fucose	1175:1192	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	24	theme	α1,2-linked	1175:1185	arg1	A					1199:1199	Con A	1195:1199	Con A	1195:1199	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	4	25	from	pigs	814:817	arg1	glands					795:800	the Brunner's glands	781:800	the Brunner's glands from No-Pro pigs	781:817	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	6	26	theme	GSA	1359:1361	arg1	lactosamine					1284:1294	lactosamine (RCA120)	1284:1303	lactosamine (RCA120)	1284:1303	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	26	theme	GSA	1359:1361	arg1	I-B4					1363:1366	GSA I-B4	1359:1366	GSA I-B4	1359:1366	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	27	dep	increase	1442:1449	arg1	iii					1434:1436	iii	1434:1436	iii	1434:1436	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	27	dep	increase	1442:1449	arg1	terminating					1267:1277	terminating	1267:1277	terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA)	1267:1426	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	27	dep	increase	1442:1449	arg1	PHA-E					1260:1264	N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase	1242:1449	PHA-E	1260:1264	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	4	28	theme	mucins	903:908	arg1	glycoproteins					846:858	neutral glycoproteins	838:858	neutral glycoproteins	838:858	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	4	28	theme	mucins	903:908	arg1	amount					872:877	a small amount	864:877	a small amount of acidic non-sulphated mucins	864:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	4	28	theme	mucins	903:908	arg1	mucins					903:908	acidic non-sulphated mucins	882:908	acidic non-sulphated mucins	882:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	9	29	theme	meat	1962:1965	arg1	quality					1967:1973	meat quality	1962:1973	meat quality	1962:1973	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	8	30	theme	probiotic	1665:1673	arg1	supplementation					1675:1689	probiotic supplementation	1665:1689	probiotic supplementation	1665:1689	These results indicate that probiotic supplementation affects the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs.					
29730471	9	31	theme	health	1865:1870	arg1	status					1872:1877	health status	1865:1877	health status	1865:1877	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	1	32	theme	periodic	317:324	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	32	theme	periodic	317:324	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	33	theme	probiotic	141:149	arg1	blend					151:155	a dietary probiotic blend	131:155	a dietary probiotic blend	131:155	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	6	34	theme	GalNAc	1475:1480	arg1	HPA					1482:1484	GalNAc HPA	1475:1484	GalNAc HPA	1475:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	3	35	theme	paraffin	744:751	arg1	wax					753:755	paraffin wax	744:755	paraffin wax	744:755	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	8	36	theme	glycan	1703:1708	arg1	composition					1710:1720	the glycan composition	1699:1720	the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs	1699:1789	These results indicate that probiotic supplementation affects the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs.					
29730471	6	37	theme	terminal	1399:1406	arg1	PNA					1423:1425	PNA	1423:1425	PNA	1423:1425	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	37	theme	terminal	1399:1406	arg1	Galβ1,3GalNAc					1408:1420	terminal Galβ1,3GalNAc	1399:1420	terminal Galβ1,3GalNAc (PNA)	1399:1426	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	4	38	theme	acidic	882:887	arg1	mucins					903:908	acidic non-sulphated mucins	882:908	acidic non-sulphated mucins	882:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	3	39	from	paraformaldehyde	666:681	arg1	fixed					619:623	fixed	619:623	fixed	619:623	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	1	40	theme	Blue	346:349	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	40	theme	Blue	346:349	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	8	41	theme	pigs	1786:1789	arg1	glands					1758:1763	the Brunner's glands	1744:1763	the Brunner's glands of growing-finishing pigs	1744:1789	These results indicate that probiotic supplementation affects the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs.					
29730471	6	42	theme	increase	1442:1449	arg1	types					1233:1237	complex types	1225:1237	complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1225:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	0	43	theme	Probiotic	0:8	arg1	supplementation					10:24	Probiotic supplementation	0:24	Probiotic supplementation	0:24	Probiotic supplementation affects the glycan composition of mucins secreted by Brunner's glands of the pig duodenum.					
29730471	6	44	dep	loss	1217:1220	arg1	ii					1211:1212	ii	1211:1212	ii	1211:1212	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	1	45	theme	pigs	269:272	arg1	duodenum					239:246	the duodenum	235:246	the duodenum of growing-finishing pigs	235:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	6	46	from	glands	1067:1072	arg1	groups					1087:1092	these two groups	1077:1092	these two groups	1077:1092	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	9	47	theme	control	2022:2028	arg1	Tufarelli					2048:2056	Tufarelli	2048:2056	Tufarelli	2048:2056	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	9	47	theme	control	2022:2028	arg1	group					2041:2045	the control basal diet group	2018:2045	the control basal diet group (Tufarelli et al., 2017)	2018:2070	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	0	48	theme	glycan	38:43	arg1	composition					45:55	the glycan composition	34:55	the glycan composition of mucins secreted by Brunner's glands of the pig duodenum	34:114	Probiotic supplementation affects the glycan composition of mucins secreted by Brunner's glands of the pig duodenum.					
29730471	7	49	theme	GlcNAc	1579:1584	arg1	residues					1586:1593	terminal GlcNAc residues	1570:1593	terminal GlcNAc residues	1570:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	6	50	contain	containing	1464:1473	arg1	O-glycans					1454:1462	O-glycans	1454:1462	O-glycans containing GalNAc HPA	1454:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	50	contain	containing	1464:1473	arg2	HPA					1482:1484	GalNAc HPA	1475:1484	GalNAc HPA	1475:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	9	51	theme	diet	2036:2039	arg1	Tufarelli					2048:2056	Tufarelli	2048:2056	Tufarelli	2048:2056	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	9	51	theme	diet	2036:2039	arg1	group					2041:2045	the control basal diet group	2018:2045	the control basal diet group (Tufarelli et al., 2017)	2018:2070	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	6	52	theme	complex	1225:1231	arg1	types					1233:1237	complex types	1225:1237	complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1225:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	53	theme	cells	1048:1052	arg1	profiles					1022:1029	lectin-binding profiles	1007:1029	lectin-binding profiles of the secretory cells of Brunner's glands in these two groups	1007:1092	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	3	54	from	embedded	732:739	arg1	wax					753:755	paraffin wax	744:755	paraffin wax	744:755	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	3	55	theme	Duodenal	588:595	arg1	fragments					604:612	Duodenal tissue fragments	588:612	Duodenal tissue fragments	588:612	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	7	56	theme	α2,6	1548:1551	arg1	sialoglycans					1553:1564	α2,6 sialoglycans	1548:1564	α2,6 sialoglycans	1548:1564	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	9	57	theme	basal	2030:2034	arg1	Tufarelli					2048:2056	Tufarelli	2048:2056	Tufarelli	2048:2056	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	9	57	theme	basal	2030:2034	arg1	group					2041:2045	the control basal diet group	2018:2045	the control basal diet group (Tufarelli et al., 2017)	2018:2070	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	6	58	theme	types	1233:1237	arg1	loss					1217:1220	a loss	1215:1220	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	59	theme	profiles	1022:1029	arg1	comparison					993:1002	A comparison	991:1002	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups	991:1092	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	9	60	theme	gastrointestinal	1835:1850	arg1	function					1852:1859	gastrointestinal function	1835:1859	gastrointestinal function	1835:1859	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	8	61	theme	growing-finishing	1768:1784	arg1	pigs					1786:1789	growing-finishing pigs	1768:1789	growing-finishing pigs	1768:1789	These results indicate that probiotic supplementation affects the glycan composition of mucins produced in the Brunner's glands of growing-finishing pigs.					
29730471	6	62	theme	N-linked	1147:1154	arg1	glycans					1156:1162	N-linked glycans	1147:1162	N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1147:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	63	from	decrease	1135:1142	arg1	glycans					1156:1162	N-linked glycans	1147:1162	N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1147:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	64	link	N-linked	1147:1154	arg1	glycans					1156:1162	N-linked glycans	1147:1162	N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1147:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	3	65	theme	phosphate-buffered-saline-buffered	631:664	arg1	paraformaldehyde					666:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	4% phosphate-buffered-saline-buffered paraformaldehyde	628:681	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	1	66	theme	blend	151:155	arg1	effect					121:126	The effect	117:126	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs	117:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	4	67	theme	secretory	762:770	arg1	cells					772:776	The secretory cells	758:776	The secretory cells of the Brunner's glands from No-Pro pigs	758:817	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	3	68	theme	4	628:628	arg1	%					629:629	%	629:629	%	629:629	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	9	69	dep	function	1852:1859	arg1	the					1831:1833	the	1831:1833	the	1831:1833	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	6	70	link	α1,3-linked	1316:1326	arg1	LTA					1336:1338	LTA	1336:1338	LTA	1336:1338	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	70	link	α1,3-linked	1316:1326	arg1	fucose					1328:1333	α1,3-linked fucose	1316:1333	α1,3-linked fucose (LTA)	1316:1339	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	71	theme	α1,3-linked	1316:1326	arg1	LTA					1336:1338	LTA	1336:1338	LTA	1336:1338	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	71	theme	α1,3-linked	1316:1326	arg1	fucose					1328:1333	α1,3-linked fucose	1316:1333	α1,3-linked fucose (LTA)	1316:1339	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	4	72	theme	neutral	838:844	arg1	glycoproteins					846:858	neutral glycoproteins	838:858	neutral glycoproteins	838:858	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	5	73	dep	opposite	935:942	arg1	that					944:947	that	944:947	that	944:947	This glycan pattern was opposite that of the Brunner's glands from Pro animals.					
29730471	9	74	theme	probiotic	1908:1916	arg1	blend					1918:1922	the probiotic blend	1904:1922	the probiotic blend	1904:1922	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	1	75	from	glands	225:230	arg1	duodenum					239:246	the duodenum	235:246	the duodenum of growing-finishing pigs	235:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	76	theme	mucins	192:197	arg1	composition					177:187	the carbohydrate composition	160:187	the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs	160:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	6	77	dep	A	1199:1199	arg1	I					1206:1206	UEA I	1202:1206	UEA I	1202:1206	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	2	78	theme	control	475:481	arg1	diet					489:492	a control basal diet	473:492	a control basal diet without the probiotic blend (No-Pro)	473:529	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	78	theme	control	475:481	arg1	treatments					461:470	two dietary treatments	449:470	two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro)	449:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	6	79	theme	Con	1195:1197	arg1	fucose					1187:1192	α1,2-linked fucose	1175:1192	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	79	theme	Con	1195:1197	arg1	A					1199:1199	Con A	1195:1199	Con A	1195:1199	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	1	80	theme	iron	364:367	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	80	theme	iron	364:367	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	4	81	theme	No-Pro	807:812	arg1	pigs					814:817	No-Pro pigs	807:817	No-Pro pigs	807:817	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	3	82	theme	graded	705:710	arg1	series					720:725	a graded alcohol series	703:725	a graded alcohol series	703:725	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	9	83	from	performance	1946:1956	arg1	group					1997:2001	the test probiotic group	1978:2001	the test probiotic group	1978:2001	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	7	84	theme	No-Pro	1487:1492	arg1	samples					1502:1508	No-Pro and Pro samples	1487:1508	No-Pro and Pro samples	1487:1508	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	6	85	theme	Pro	1109:1111	arg1	pigs					1113:1116	Pro pigs	1109:1116	Pro pigs	1109:1116	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	5	86	theme	Pro	978:980	arg1	animals					982:988	Pro animals	978:988	Pro animals	978:988	This glycan pattern was opposite that of the Brunner's glands from Pro animals.					
29730471	7	87	from	affinity	1602:1609	arg1	expression					1534:1543	the expression	1530:1543	the expression of α2,6 sialoglycans and terminal GlcNAc residues	1530:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	4	88	theme	non-sulphated	889:901	arg1	mucins					903:908	acidic non-sulphated mucins	882:908	acidic non-sulphated mucins	882:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	6	89	from	increase	1442:1449	arg1	O-glycans					1454:1462	O-glycans	1454:1462	O-glycans containing GalNAc HPA	1454:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	1	90	theme	dietary	133:139	arg1	blend					151:155	a dietary probiotic blend	131:155	a dietary probiotic blend	131:155	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	0	91	theme	duodenum	107:114	arg1	glands					89:94	Brunner's glands	79:94	Brunner's glands of the pig duodenum	79:114	Probiotic supplementation affects the glycan composition of mucins secreted by Brunner's glands of the pig duodenum.					
29730471	4	92	theme	small	866:870	arg1	mucins					903:908	acidic non-sulphated mucins	882:908	acidic non-sulphated mucins	882:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	4	92	theme	small	866:870	arg1	amount					872:877	a small amount	864:877	a small amount of acidic non-sulphated mucins	864:908	The secretory cells of the Brunner's glands from No-Pro pigs primarily produced neutral glycoproteins and a small amount of acidic non-sulphated mucins.					
29730471	2	93	theme	probiotic	565:573	arg1	blend					575:579	the probiotic blend	561:579	the probiotic blend (Pro)	561:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	93	theme	probiotic	565:573	arg1	Pro					582:584	Pro	582:584	Pro	582:584	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	9	94	theme	probiotic	1987:1995	arg1	group					1997:2001	the test probiotic group	1978:2001	the test probiotic group	1978:2001	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	0	95	theme	pig	103:105	arg1	duodenum					107:114	the pig duodenum	99:114	the pig duodenum	99:114	Probiotic supplementation affects the glycan composition of mucins secreted by Brunner's glands of the pig duodenum.					
29730471	1	96	theme	Alcian	339:344	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	96	theme	Alcian	339:344	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	2	97	theme	dietary	453:459	arg1	diet					489:492	a control basal diet	473:492	a control basal diet without the probiotic blend (No-Pro)	473:529	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	97	theme	dietary	453:459	arg1	treatments					461:470	two dietary treatments	449:470	two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro)	449:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	97	theme	dietary	453:459	arg1	diet					542:545	a test diet	535:545	a test diet that included the probiotic blend (Pro)	535:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	5	98	from	animals	982:988	arg1	glands					966:971	the Brunner's glands	952:971	the Brunner's glands from Pro animals	952:988	This glycan pattern was opposite that of the Brunner's glands from Pro animals.					
29730471	1	99	theme	pH	351:352	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	99	theme	pH	351:352	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	100	from	effect	121:126	arg1	composition					177:187	the carbohydrate composition	160:187	the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs	160:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	6	101	contain	containing	1164:1173	arg1	glycans					1156:1162	N-linked glycans	1147:1162	N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1147:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	101	contain	containing	1164:1173	arg2	A					1199:1199	Con A	1195:1199	Con A	1195:1199	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	101	contain	containing	1164:1173	arg2	fucose					1187:1192	α1,2-linked fucose	1175:1192	α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1175:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	9	102	theme	test	1982:1985	arg1	group					1997:2001	the test probiotic group	1978:2001	the test probiotic group	1978:2001	These changes could effectively act on the gastrointestinal function and health status of these animals because the probiotic blend induced higher growth performance and meat quality in the test probiotic group than it did in the control basal diet group (Tufarelli et al., 2017).					
29730471	6	103	theme	PHA-L	1253:1257	arg1	increase					1442:1449	N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase	1242:1449	increase	1442:1449	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	1	104	theme	growing-finishing	251:267	arg1	pigs					269:272	growing-finishing pigs	251:272	growing-finishing pigs	251:272	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	2	105	dep	treatments	461:470	arg1	diet					489:492	a control basal diet	473:492	a control basal diet without the probiotic blend (No-Pro)	473:529	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	105	dep	treatments	461:470	arg1	diet					542:545	a test diet	535:545	a test diet that included the probiotic blend (Pro)	535:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	2	105	dep	treatments	461:470	arg1	treatments					461:470	two dietary treatments	449:470	two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro)	449:585	Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro).					
29730471	1	106	dep	conventional	303:314	arg1	staining					377:384	Alcian Blue pH 2.5, high iron diamine staining	339:384	staining	377:384	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	1	106	dep	conventional	303:314	arg1	acid-Schiff					326:336	periodic acid-Schiff	317:336	periodic acid-Schiff	317:336	The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry.					
29730471	3	107	from	wax	753:755	arg1	embedded					732:739	embedded	732:739	embedded	732:739	Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax.					
29730471	6	108	theme	N-glycans	1242:1250	arg1	increase					1442:1449	N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase	1242:1449	increase	1442:1449	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	6	109	from	O-glycans	1454:1462	arg1	types					1233:1237	complex types	1225:1237	complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA	1225:1484	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	0	110	theme	mucins	60:65	arg1	composition					45:55	the glycan composition	34:55	the glycan composition of mucins secreted by Brunner's glands of the pig duodenum	34:114	Probiotic supplementation affects the glycan composition of mucins secreted by Brunner's glands of the pig duodenum.					
29730471	7	111	theme	residues	1586:1593	arg1	expression					1534:1543	the expression	1530:1543	the expression of α2,6 sialoglycans and terminal GlcNAc residues	1530:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	5	112	theme	glycan	916:921	arg1	pattern					923:929	This glycan pattern	911:929	This glycan pattern	911:929	This glycan pattern was opposite that of the Brunner's glands from Pro animals.					
29730471	7	113	theme	terminal	1570:1577	arg1	residues					1586:1593	terminal GlcNAc residues	1570:1593	terminal GlcNAc residues	1570:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	6	114	theme	secretory	1038:1046	arg1	cells					1048:1052	the secretory cells	1034:1052	the secretory cells of Brunner's glands in these two groups	1034:1092	A comparison of lectin-binding profiles of the secretory cells of Brunner's glands in these two groups showed that in Pro pigs, there was (i) a decrease in N-linked glycans containing α1,2-linked fucose (Con A, UEA I); (ii) a loss of complex types of N-glycans (PHA-L, PHA-E) terminating with lactosamine (RCA120), α1,6- and α1,3-linked fucose (LTA), and α-galactose (GSA I-B4), as well as of O-glycans with terminal Galβ1,3GalNAc (PNA); and (iii) an increase in O-glycans containing GalNAc HPA.					
29730471	7	115	from	change	1520:1525	arg1	expression					1534:1543	the expression	1530:1543	the expression of α2,6 sialoglycans and terminal GlcNAc residues	1530:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
29730471	7	116	theme	sialoglycans	1553:1564	arg1	expression					1534:1543	the expression	1530:1543	the expression of α2,6 sialoglycans and terminal GlcNAc residues	1530:1593	No-Pro and Pro samples showed no change in the expression of α2,6 sialoglycans and terminal GlcNAc residues and no affinity for MAL II, DBA, and SBA.					
31877766	5	0	theme	operating	682:690	arg1	curve					708:712	the receiver operating characteristics curve	669:712	the receiver operating characteristics curve	669:712	The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients.					
31877766	4	1	theme	full-length-OPN	469:483	arg1	levels					485:490	The urinary full-length-OPN levels	457:490	The urinary full-length-OPN levels	457:490	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	2	2	from	role	166:169	arg1	urolithiasis					207:218	urolithiasis	207:218	urolithiasis	207:218	This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis.					
31877766	6	3	theme	p	961:961	arg1	volunteers					949:958	the healthy volunteers	937:958	the healthy volunteers (p < 0.0001)	937:971	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	3	theme	p	961:961	arg1	<					963:963	p < 0.0001	961:970	p < 0.0001	961:970	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	7	4	theme	urinary	1134:1140	arg1	full-length-OPN					1142:1156	urinary full-length-OPN	1134:1156	urinary full-length-OPN in stone forming urolithiasis patients	1134:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	9	5	used	used	1613:1616	arg2	levels					1597:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	9	5	used	used	1613:1616	arg2	biomarker					1636:1644	a urolithiasis biomarker	1621:1644	a urolithiasis biomarker	1621:1644	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	3	6	theme	full-length-OPN	296:310	arg1	levels					312:317	urinary full-length-OPN levels	288:317	urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers	288:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	8	7	theme	longitudinal	1288:1299	arg1	study					1311:1315	the prospective longitudinal follow-up study	1272:1315	the prospective longitudinal follow-up study	1272:1315	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	7	8	theme	urinary	1098:1104	arg1	full-length-OPN					1106:1120	urinary full-length-OPN	1098:1120	urinary full-length-OPN	1098:1120	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	9	theme	Gal3C-S-	830:837	arg1	OPN/full-length-OPN					840:858	urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN,	796:859	OPN/full-length-OPN	840:858	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	8	10	contain	had	1361:1363	arg2	levels					1393:1398	Gal3C-S-OPN/full-length-OPN levels	1365:1398	Gal3C-S-OPN/full-length-OPN levels below the cutoff value	1365:1421	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	8	10	contain	had	1361:1363	arg1	%					1322:1322	92.8%	1318:1322	92.8% of the stone-free urolithiasis group	1318:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	8	10	contain	had	1361:1363	arg1	group					1355:1359	the stone-free urolithiasis group	1327:1359	the stone-free urolithiasis group	1327:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	7	11	theme	esculentum	1068:1077	arg1	lectin					1079:1084	Lycopersicon esculentum lectin	1055:1084	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN	1051:1120	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	2	12	theme	OPN	200:202	arg1	glycosylation					183:195	aberrant glycosylation	174:195	aberrant glycosylation of OPN	174:202	This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis.					
31877766	6	13	theme	good	979:982	arg1	discrimination					984:997	good discrimination	979:997	good discrimination (AUC, 0.953)	979:1010	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	3	14	theme	urinary	288:294	arg1	levels					312:317	urinary full-length-OPN levels	288:317	urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers	288:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	8	15	theme	urolithiasis	1498:1509	arg1	group					1511:1515	the residual-stone urolithiasis group	1479:1515	the residual-stone urolithiasis group	1479:1515	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	3	16	from	levels	312:317	arg1	volunteers					364:373	157 healthy volunteers	352:373	157 healthy volunteers	352:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	3	16	from	levels	312:317	arg1	patients					339:346	110 urolithiasis patients	322:346	110 urolithiasis patients	322:346	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	2	17	theme	glycosylation	183:195	arg1	role					166:169	the role	162:169	the role of aberrant glycosylation of OPN in urolithiasis	162:218	This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis.					
31877766	1	18	theme	matrix	94:99	arg1	glycoprotein					101:112	a matrix glycoprotein	92:112	a matrix glycoprotein of urinary calculi	92:131	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	1	18	theme	matrix	94:99	arg1	Osteopontin					71:81	Osteopontin	71:81	Osteopontin (OPN)	71:87	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	8	19	theme	stone-free	1331:1340	arg1	group					1355:1359	the stone-free urolithiasis group	1327:1359	the stone-free urolithiasis group	1327:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	5	20	theme	forming	736:742	arg1	patients					757:764	stone forming urolithiasis patients	730:764	stone forming urolithiasis patients	730:764	The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients.					
31877766	8	21	theme	group	1355:1359	arg1	group					1355:1359	the stone-free urolithiasis group	1327:1359	the stone-free urolithiasis group	1327:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	8	21	theme	group	1355:1359	arg1	%					1322:1322	92.8%	1318:1322	92.8% of the stone-free urolithiasis group	1318:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	0	22	theme	Stone	54:58	arg1	Formation					60:68	Urinary Stone Formation	46:68	Urinary Stone Formation	46:68	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	1	23	theme	calculi	125:131	arg1	glycoprotein					101:112	a matrix glycoprotein	92:112	a matrix glycoprotein of urinary calculi	92:131	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	1	23	theme	calculi	125:131	arg1	Osteopontin					71:81	Osteopontin	71:81	Osteopontin (OPN)	71:87	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	4	24	theme	immunosorbent	526:538	arg1	assay					540:544	enzyme-linked immunosorbent assay	512:544	enzyme-linked immunosorbent assay	512:544	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	8	25	theme	Gal3C-S-OPN/full-length-OPN	1365:1391	arg1	levels					1393:1398	Gal3C-S-OPN/full-length-OPN levels	1365:1398	Gal3C-S-OPN/full-length-OPN levels below the cutoff value	1365:1421	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	7	26	theme	healthy	1249:1255	arg1	volunteers					1257:1266	healthy volunteers	1249:1266	healthy volunteers	1249:1266	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	27	theme	90	1013:1014	arg1	%					1015:1015	%	1015:1015	%	1015:1015	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	8	28	theme	cutoff	1410:1415	arg1	value					1417:1421	the cutoff value	1406:1421	the cutoff value	1406:1421	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	0	29	theme	Osteopontin	31:41	arg1	Glycosylation					14:26	Glycosylation	14:26	Glycosylation of Osteopontin	14:41	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	9	30	theme	urolithiasis	1623:1634	arg1	biomarker					1636:1644	a urolithiasis biomarker	1621:1644	a urolithiasis biomarker	1621:1644	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	9	30	theme	urolithiasis	1623:1634	arg1	levels					1597:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	3	31	theme	longitudinal	410:421	arg1	follow-up					423:431	longitudinal follow-up	410:431	longitudinal follow-up during stone treatment	410:454	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	6	32	theme	%	1036:1036	arg1	specificity					1038:1048	92% specificity	1034:1048	92% specificity	1034:1048	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	33	theme	lectin	812:817	arg1	OPN/full-length-OPN					840:858	urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN,	796:859	OPN/full-length-OPN	840:858	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	7	34	theme	forming	1167:1173	arg1	patients					1188:1195	stone forming urolithiasis patients	1161:1195	stone forming urolithiasis patients	1161:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	35	theme	forming	899:905	arg1	patients					920:927	the stone forming urolithiasis patients	889:927	the stone forming urolithiasis patients	889:927	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	0	36	gly	Glycosylation	14:26	arg1	Osteopontin					31:41	Osteopontin	31:41	Osteopontin	31:41	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	6	37	theme	urinary	796:802	arg1	OPN/full-length-OPN					840:858	urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN,	796:859	OPN/full-length-OPN	840:858	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	7	38	located	observed	1237:1244	arg2	structure					1214:1222	a polyLacNAc structure	1201:1222	a polyLacNAc structure that was not observed in healthy volunteers	1201:1266	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	7	38	located	observed	1237:1244	arg1	volunteers					1257:1266	healthy volunteers	1249:1266	healthy volunteers	1249:1266	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	0	39	theme	Urinary	46:52	arg1	Formation					60:68	Urinary Stone Formation	46:68	Urinary Stone Formation	46:68	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	4	40	theme	lectin	605:610	arg1	blotting					612:619	lectin blotting	605:619	lectin blotting	605:619	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	5	41	theme	characteristics	692:706	arg1	curve					708:712	the receiver operating characteristics curve	669:712	the receiver operating characteristics curve	669:712	The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients.					
31877766	3	42	gly	glycosylated	257:268	arg1	OPN					270:272	urinary glycosylated OPN	249:272	urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers	249:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	7	43	theme	full-length-OPN	1106:1120	arg1	analysis					1086:1093	The Lycopersicon esculentum lectin analysis	1051:1093	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN	1051:1120	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	44	theme	healthy	941:947	arg1	volunteers					949:958	the healthy volunteers	937:958	the healthy volunteers (p < 0.0001)	937:971	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	44	theme	healthy	941:947	arg1	<					963:963	p < 0.0001	961:970	p < 0.0001	961:970	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	7	45	contain	had	1197:1199	arg2	structure					1214:1222	a polyLacNAc structure	1201:1222	a polyLacNAc structure that was not observed in healthy volunteers	1201:1266	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	7	45	contain	had	1197:1199	arg1	full-length-OPN					1142:1156	urinary full-length-OPN	1134:1156	urinary full-length-OPN in stone forming urolithiasis patients	1134:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	4	46	gly	glycosylated	550:561	arg1	OPN					563:565	glycosylated OPN	550:565	glycosylated OPN	550:565	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	4	47	theme	glycosylated	550:561	arg1	OPN					563:565	glycosylated OPN	550:565	glycosylated OPN	550:565	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	8	48	theme	prospective	1276:1286	arg1	study					1311:1315	the prospective longitudinal follow-up study	1272:1315	the prospective longitudinal follow-up study	1272:1315	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	5	49	theme	urolithiasis	744:755	arg1	patients					757:764	stone forming urolithiasis patients	730:764	stone forming urolithiasis patients	730:764	The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients.					
31877766	4	50	theme	urinary	461:467	arg1	levels					485:490	The urinary full-length-OPN levels	457:490	The urinary full-length-OPN levels	457:490	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	8	51	theme	follow-up	1301:1309	arg1	study					1311:1315	the prospective longitudinal follow-up study	1272:1315	the prospective longitudinal follow-up study	1272:1315	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	7	52	theme	Lycopersicon	1055:1066	arg1	lectin					1079:1084	Lycopersicon esculentum lectin	1055:1084	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN	1051:1120	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	4	53	link	enzyme-linked	512:524	arg1	assay					540:544	enzyme-linked immunosorbent assay	512:544	enzyme-linked immunosorbent assay	512:544	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	3	54	theme	urinary	249:255	arg1	OPN					270:272	urinary glycosylated OPN	249:272	urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers	249:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	8	55	theme	group	1511:1515	arg1	group					1511:1515	the residual-stone urolithiasis group	1479:1515	the residual-stone urolithiasis group	1479:1515	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	8	55	theme	group	1511:1515	arg1	%					1474:1474	71.4%	1470:1474	71.4% of the residual-stone urolithiasis group	1470:1515	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	2	56	gly	glycosylation	183:195	arg1	OPN					200:202	OPN	200:202	OPN	200:202	This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis.					
31877766	7	57	theme	lectin	1079:1084	arg1	analysis					1086:1093	The Lycopersicon esculentum lectin analysis	1051:1093	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN	1051:1120	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	5	58	theme	stone	730:734	arg1	patients					757:764	stone forming urolithiasis patients	730:764	stone forming urolithiasis patients	730:764	The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients.					
31877766	6	59	theme	92	1034:1035	arg1	%					1036:1036	%	1036:1036	%	1036:1036	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	8	60	theme	decreased	1530:1538	arg1	levels					1540:1545	decreased levels	1530:1545	decreased levels after URS	1530:1555	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	1	61	theme	urinary	117:123	arg1	calculi					125:131	urinary calculi	117:131	urinary calculi	117:131	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	3	62	theme	urolithiasis	326:337	arg1	patients					339:346	110 urolithiasis patients	322:346	110 urolithiasis patients	322:346	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	8	63	theme	urolithiasis	1342:1353	arg1	group					1355:1359	the stone-free urolithiasis group	1327:1359	the stone-free urolithiasis group	1327:1359	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	9	64	theme	Gal3C-S-OPN/full-length-OPN	1569:1595	arg1	biomarker					1636:1644	a urolithiasis biomarker	1621:1644	a urolithiasis biomarker	1621:1644	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	9	64	theme	Gal3C-S-OPN/full-length-OPN	1569:1595	arg1	levels					1597:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Gal3C-S-OPN/full-length-OPN levels	1569:1602	Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker.					
31877766	6	65	theme	%	1015:1015	arg1	sensitivity					1017:1027	90% sensitivity	1013:1027	90% sensitivity	1013:1027	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	0	66	from	Impact	4:9	arg1	Formation					60:68	Urinary Stone Formation	46:68	Urinary Stone Formation	46:68	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	8	67	theme	residual-stone	1483:1496	arg1	group					1511:1515	the residual-stone urolithiasis group	1479:1515	the residual-stone urolithiasis group	1479:1515	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	3	68	theme	healthy	356:362	arg1	volunteers					364:373	157 healthy volunteers	352:373	157 healthy volunteers	352:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	4	69	theme	enzyme-linked	512:524	arg1	assay					540:544	enzyme-linked immunosorbent assay	512:544	enzyme-linked immunosorbent assay	512:544	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	7	70	theme	polyLacNAc	1203:1212	arg1	structure					1214:1222	a polyLacNAc structure	1201:1222	a polyLacNAc structure that was not observed in healthy volunteers	1201:1266	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	4	71	theme	lectin	588:593	arg1	array					595:599	a lectin array	586:599	a lectin array	586:599	The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting.					
31877766	3	72	theme	stone	440:444	arg1	treatment					446:454	stone treatment	440:454	stone treatment	440:454	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	0	73	theme	Glycosylation	14:26	arg1	Impact					4:9	The Impact	0:9	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation	0:68	The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.					
31877766	8	74	theme	ureteroscopic	1429:1441	arg1	URS					1456:1458	URS	1456:1458	URS	1456:1458	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	8	74	theme	ureteroscopic	1429:1441	arg1	lithotripsy					1443:1453	ureteroscopic lithotripsy	1429:1453	ureteroscopic lithotripsy (URS)	1429:1459	In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS.					
31877766	7	75	theme	urolithiasis	1175:1186	arg1	patients					1188:1195	stone forming urolithiasis patients	1161:1195	stone forming urolithiasis patients	1161:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	76	theme	reactive-	819:827	arg1	OPN/full-length-OPN					840:858	urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN,	796:859	OPN/full-length-OPN	840:858	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	3	77	theme	glycosylated	257:268	arg1	OPN					270:272	urinary glycosylated OPN	249:272	urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers	249:373	We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment.					
31877766	7	78	theme	stone	1161:1165	arg1	patients					1188:1195	stone forming urolithiasis patients	1161:1195	stone forming urolithiasis patients	1161:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	6	79	theme	urolithiasis	907:918	arg1	patients					920:927	the stone forming urolithiasis patients	889:927	the stone forming urolithiasis patients	889:927	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	1	80	gly	glycoprotein	101:112	arg1	glycoprotein					101:112	a matrix glycoprotein	92:112	a matrix glycoprotein of urinary calculi	92:131	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	1	80	gly	glycoprotein	101:112	arg1	Osteopontin					71:81	Osteopontin	71:81	Osteopontin (OPN)	71:87	Osteopontin (OPN) is a matrix glycoprotein of urinary calculi.					
31877766	7	81	from	full-length-OPN	1142:1156	arg1	patients					1188:1195	stone forming urolithiasis patients	1161:1195	stone forming urolithiasis patients	1161:1195	The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers.					
31877766	2	82	theme	aberrant	174:181	arg1	glycosylation					183:195	aberrant glycosylation	174:195	aberrant glycosylation of OPN	174:202	This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis.					
31877766	6	83	theme	Gal3C-S	804:810	arg1	OPN/full-length-OPN					840:858	urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN,	796:859	OPN/full-length-OPN	840:858	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	84	dep	discrimination	984:997	arg1	AUC					1000:1002	AUC	1000:1002	AUC	1000:1002	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	84	dep	discrimination	984:997	arg1	0.953					1005:1009	0.953	1005:1009	0.953	1005:1009	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	85	theme	stone	893:897	arg1	patients					920:927	the stone forming urolithiasis patients	889:927	the stone forming urolithiasis patients	889:927	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
31877766	6	86	theme	retrospective	774:786	arg1	cohort					788:793	the retrospective cohort	770:793	the retrospective cohort	770:793	In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity.					
29508223	4	0	theme	6-antenna	962:970	arg1	composition					943:953	the composition	939:953	the composition of the 6-antenna of several of the glycans	939:996	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	4	1	theme	fragment	781:788	arg1	cross-sections					800:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	1	2	theme	traveling-wave	175:188	arg1	separation					203:212	traveling-wave ion mobility separation	175:212	traveling-wave ion mobility separation	175:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	3	3	from	residue	653:659	arg1	isomers					676:682	Man8GlcNAc2 isomers	664:682	Man8GlcNAc2 isomers	664:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	1	4	theme	N-linked	284:291	arg1	glycans					293:299	N-linked glycans	284:299	N-linked glycans	284:299	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	5	5	theme	Potential	1096:1104	arg1	mechanisms					1106:1115	Potential mechanisms	1096:1115	Potential mechanisms for the formation of the various ions	1096:1153	Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.					
29508223	4	6	theme	several	975:981	arg1	6-antenna					962:970	the 6-antenna	958:970	the 6-antenna of several of the glycans	958:996	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	2	7	theme	glycans	332:338	arg1	Fragmentation					302:314	Fragmentation	302:314	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources,	302:411	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	4	8	theme	glycans	841:847	arg1	antennae					854:861	the antennae	850:861	the antennae	850:861	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	4	8	theme	glycans	841:847	arg1	identification					823:836	identification	823:836	identification of glycans, the antennae of which terminated in the antigenic α-galactose residue	823:918	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	5	9	theme	estimated	1177:1185	arg1	cross-sections					1199:1212	the estimated collisional cross-sections	1173:1212	the estimated collisional cross-sections	1173:1212	Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.					
29508223	4	10	from	ions	1061:1064	arg1	cross-sections					1032:1045	different cross-sections	1022:1045	different cross-sections from isomeric ions produced in the same spectra	1022:1093	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	2	11	theme	high-mannose	319:330	arg1	glycans					332:338	high-mannose glycans	319:338	high-mannose glycans released from several glycoproteins, including those from viral sources,	319:411	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	4	12	theme	Specific	772:779	arg1	cross-sections					800:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	5	13	theme	collisional	1187:1197	arg1	cross-sections					1199:1212	the estimated collisional cross-sections	1173:1212	the estimated collisional cross-sections	1173:1212	Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.					
29508223	1	14	link	N-linked	284:291	arg1	glycans					293:299	N-linked glycans	284:299	N-linked glycans	284:299	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	3	15	theme	cross-ring	578:587	arg1	fragments					589:597	cross-ring fragments	578:597	cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	578:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	3	16	from	isomers	676:682	arg1	cleavage					612:619	cleavage	612:619	cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	612:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	1	17	theme	ion	190:192	arg1	separation					203:212	traveling-wave ion mobility separation	175:212	traveling-wave ion mobility separation	175:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	0	18	from	Separation	37:46	arg1	Information					7:17	Information	7:17	Information	7:17	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	1	19	theme	Extracted	82:90	arg1	distributions					105:117	Extracted arrival time distributions	82:117	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	82:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	1	20	theme	mobility	194:201	arg1	separation					203:212	traveling-wave ion mobility separation	175:212	traveling-wave ion mobility separation	175:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	1	21	theme	arrival	92:98	arg1	distributions					105:117	Extracted arrival time distributions	82:117	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	82:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	1	22	theme	time	100:103	arg1	distributions					105:117	Extracted arrival time distributions	82:117	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	82:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	0	23	theme	Ion	24:26	arg1	Separation					37:46	Ion Mobility Separation	24:46	Ion Mobility Separation of High-Mannose Glycan Fragments	24:79	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	4	24	theme	α-galactose	900:910	arg1	residue					912:918	the antigenic α-galactose residue	886:918	the antigenic α-galactose residue	886:918	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	4	25	contain	have	1017:1020	arg2	cross-sections					1032:1045	different cross-sections	1022:1045	different cross-sections from isomeric ions produced in the same spectra	1022:1093	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	4	25	contain	have	1017:1020	arg1	ions					925:928	ions	925:928	ions defining the composition of the 6-antenna of several of the glycans	925:996	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	2	26	theme	several	354:360	arg1	those					387:391	those	387:391	those	387:391	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	26	theme	several	354:360	arg1	glycoproteins					362:374	several glycoproteins	354:374	several glycoproteins	354:374	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	27	attach	released	340:347	arg1	those					387:391	those	387:391	those	387:391	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	27	attach	released	340:347	arg2	glycans					332:338	high-mannose glycans	319:338	high-mannose glycans released from several glycoproteins, including those from viral sources,	319:411	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	27	attach	released	340:347	arg1	glycoproteins					362:374	several glycoproteins	354:374	several glycoproteins	354:374	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	28	theme	viral	398:402	arg1	sources					404:410	viral sources	398:410	viral sources	398:410	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	29	theme	additional	507:516	arg1	information					518:528	additional information	507:528	additional information used to assign structural isomers	507:562	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	4	30	theme	collision	790:798	arg1	cross-sections					800:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections	772:813	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	4	31	theme	different	1022:1030	arg1	cross-sections					1032:1045	different cross-sections	1022:1045	different cross-sections from isomeric ions produced in the same spectra	1022:1093	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	6	32	theme	Graphical	1229:1237	arg1	Abstract					1239:1246	Graphical Abstract	1229:1246	Graphical Abstract	1229:1246	Graphical Abstract ᅟ.					
29508223	3	33	theme	residue	653:659	arg1	cleavage					612:619	cleavage	612:619	cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	612:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	3	34	theme	reducing	628:635	arg1	residue					653:659	the reducing terminal GlcNAc residue	624:659	the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	624:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	0	35	theme	Mobility	28:35	arg1	Separation					37:46	Ion Mobility Separation	24:46	Ion Mobility Separation of High-Mannose Glycan Fragments	24:79	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	4	36	theme	same	1082:1085	arg1	spectra					1087:1093	the same spectra	1078:1093	the same spectra	1078:1093	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	3	37	theme	collision	696:704	arg1	cross-sections					706:719	unique collision cross-sections	689:719	unique collision cross-sections	689:719	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	2	38	theme	collisional	467:477	arg1	cross-sections					479:492	unique collisional cross-sections	460:492	unique collisional cross-sections	460:492	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	0	39	theme	High-Mannose	51:62	arg1	Fragments					71:79	High-Mannose Glycan Fragments	51:79	High-Mannose Glycan Fragments	51:79	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	1	40	link	CID-derived	135:145	arg1	fragments					147:155	negative ion CID-derived fragments	122:155	negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	122:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	2	41	gly	glycoproteins	362:374	arg1	those					387:391	those	387:391	those	387:391	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	41	gly	glycoproteins	362:374	arg1	glycoproteins					362:374	several glycoproteins	354:374	several glycoproteins	354:374	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	3	42	theme	terminal	637:644	arg1	residue					653:659	the reducing terminal GlcNAc residue	624:659	the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	624:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	1	43	theme	negative	122:129	arg1	fragments					147:155	negative ion CID-derived fragments	122:155	negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	122:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	1	44	from	information	269:279	arg1	glycans					293:299	N-linked glycans	284:299	N-linked glycans	284:299	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	3	45	theme	GlcNAc	646:651	arg1	residue					653:659	the reducing terminal GlcNAc residue	624:659	the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	624:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	1	46	theme	ion	131:133	arg1	fragments					147:155	negative ion CID-derived fragments	122:155	negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	122:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	3	47	from	cleavage	612:619	arg1	isomers					676:682	Man8GlcNAc2 isomers	664:682	Man8GlcNAc2 isomers	664:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	4	48	theme	antigenic	890:898	arg1	residue					912:918	the antigenic α-galactose residue	886:918	the antigenic α-galactose residue	886:918	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	1	49	theme	CID-derived	135:145	arg1	fragments					147:155	negative ion CID-derived fragments	122:155	negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	122:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	4	50	theme	glycans	990:996	arg1	several					975:981	several	975:981	several	975:981	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	5	51	theme	various	1142:1148	arg1	ions					1150:1153	the various ions	1138:1153	the various ions	1138:1153	Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.					
29508223	3	52	theme	unique	689:694	arg1	cross-sections					706:719	unique collision cross-sections	689:719	unique collision cross-sections	689:719	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	1	53	theme	fragments	147:155	arg1	distributions					105:117	Extracted arrival time distributions	82:117	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation	82:212	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	4	54	theme	isomeric	1052:1059	arg1	ions					1061:1064	isomeric ions	1052:1064	isomeric ions produced in the same spectra	1052:1093	Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra.					
29508223	0	55	theme	Fragments	71:79	arg1	Separation					37:46	Ion Mobility Separation	24:46	Ion Mobility Separation of High-Mannose Glycan Fragments	24:79	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	5	56	theme	ions	1150:1153	arg1	formation					1125:1133	the formation	1121:1133	the formation of the various ions	1121:1153	Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.					
29508223	3	57	theme	Man8GlcNAc2	664:674	arg1	isomers					676:682	Man8GlcNAc2 isomers	664:682	Man8GlcNAc2 isomers	664:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	2	58	theme	fragments	430:438	arg1	fragments					430:438	50 fragments	427:438	50 fragments	427:438	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	58	theme	fragments	430:438	arg1	many					441:444	many	441:444	many	441:444	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	1	59	theme	structural	258:267	arg1	information					269:279	structural information	258:279	structural information on N-linked glycans	258:299	Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans.					
29508223	0	60	theme	Glycan	64:69	arg1	Fragments					71:79	High-Mannose Glycan Fragments	51:79	High-Mannose Glycan Fragments	51:79	Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.					
29508223	2	61	theme	unique	460:465	arg1	cross-sections					479:492	unique collisional cross-sections	460:492	unique collisional cross-sections	460:492	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	2	62	theme	structural	545:554	arg1	isomers					556:562	structural isomers	545:562	structural isomers	545:562	Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers.					
29508223	3	63	contain	have	684:687	arg1	fragments					589:597	cross-ring fragments	578:597	cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers	578:682	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
29508223	3	63	contain	have	684:687	arg2	cross-sections					706:719	unique collision cross-sections	689:719	unique collision cross-sections	689:719	For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures.					
31260704	7	0	theme	capillary	1270:1278	arg1	electrophoresis					1284:1298	capillary gel electrophoresis	1270:1298	capillary gel electrophoresis with laser-induced fluorescence detection	1270:1340	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	4	1	theme	galactose	759:767	arg1	variation					733:741	the day-by-day variation	718:741	the day-by-day variation of the terminal galactose within two major N-glycoforms	718:797	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	5	2	theme	mass	838:841	arg1	MAMS					857:860	MAMS	857:860	MAMS	857:860	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	5	2	theme	mass	838:841	arg1	spectrometry					843:854	mass spectrometry	838:854	mass spectrometry (MAMS)	838:861	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	3	3	theme	IgG1	535:538	arg1	N-glycopeptides					516:530	N-glycopeptides	516:530	N-glycopeptides of IgG1 produced in a perfusion cell culture	516:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	8	4	theme	recombinant	1470:1480	arg1	proteins					1482:1489	recombinant proteins	1470:1489	recombinant proteins	1470:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	7	5	theme	observed	1213:1220	arg1	trend					1222:1226	The observed trend	1209:1226	The observed trend of released N-glycans	1209:1248	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	3	6	theme	cell	564:567	arg1	culture					569:575	a perfusion cell culture	552:575	a perfusion cell culture	552:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	6	7	theme	bioreactor	1189:1198	arg1	process					1200:1206	the bioreactor process	1185:1206	the bioreactor process	1185:1206	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	4	8	theme	interaction	638:648	arg1	chromatography					657:670	zwitterionic-hydrophilic interaction liquid chromatography	613:670	zwitterionic-hydrophilic interaction liquid chromatography for sample purification	613:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	7	9	theme	fluorescence	1319:1330	arg1	detection					1332:1340	laser-induced fluorescence detection	1305:1340	laser-induced fluorescence detection	1305:1340	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	7	10	theme	laser-induced	1305:1317	arg1	detection					1332:1340	laser-induced fluorescence detection	1305:1340	laser-induced fluorescence detection	1305:1340	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	4	11	theme	major	780:784	arg1	N-glycoforms					786:797	two major N-glycoforms	776:797	two major N-glycoforms	776:797	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	7	12	theme	N-glycans	1240:1248	arg1	trend					1222:1226	The observed trend	1209:1226	The observed trend of released N-glycans	1209:1248	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	6	13	theme	spectral	951:958	arg1	repeatability					960:972	The spectral repeatability	947:972	The spectral repeatability	947:972	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	2	14	theme	product	280:286	arg1	monitoring					288:297	product monitoring	280:297	product monitoring	280:297	Therefore, engineering and production of the mAb is highly regulated and requires product monitoring, especially in terms of N-glycosylation patterns.					
31260704	8	15	theme	glycosylation	1415:1427	arg1	control					1429:1435	glycosylation control	1415:1435	glycosylation control during the production process of recombinant proteins	1415:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	4	16	theme	zwitterionic-hydrophilic	613:636	arg1	chromatography					657:670	zwitterionic-hydrophilic interaction liquid chromatography	613:670	zwitterionic-hydrophilic interaction liquid chromatography for sample purification	613:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	3	17	theme	perfusion	554:562	arg1	culture					569:575	a perfusion cell culture	552:575	a perfusion cell culture	552:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	6	18	theme	N-glycosylation	1114:1128	arg1	variability					1130:1140	the N-glycosylation variability	1110:1140	the N-glycosylation variability resulting from operating conditions	1110:1176	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	3	19	theme	high-throughput	376:390	arg1	method					465:470	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method	374:470	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	7	20	theme	gel	1280:1282	arg1	electrophoresis					1284:1298	capillary gel electrophoresis	1270:1298	capillary gel electrophoresis with laser-induced fluorescence detection	1270:1340	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	3	21	gly	N-glycopeptides	516:530	arg1	IgG1					535:538	IgG1	535:538	IgG1 produced in a perfusion cell culture	535:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	21	gly	N-glycopeptides	516:530	arg2	N-glycopeptides					516:530	N-glycopeptides	516:530	N-glycopeptides of IgG1 produced in a perfusion cell culture	516:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	5	22	theme	carbohydrate	920:931	arg1	distribution					933:944	the carbohydrate distribution	916:944	the carbohydrate distribution	916:944	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	3	23	theme	matrix-assisted	392:406	arg1	MALDI-MS					455:462	MALDI-MS	455:462	MALDI-MS	455:462	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	23	theme	matrix-assisted	392:406	arg1	spectrometry					441:452	matrix-assisted laser desorption/ionization mass spectrometry	392:452	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	1	24	theme	manufactured	107:118	arg1	mAb					143:145	mAb	143:145	mAb	143:145	Biologically manufactured monoclonal antibodies (mAb) can strongly vary in their efficacy and affinity.					
31260704	1	24	theme	manufactured	107:118	arg1	antibodies					131:140	monoclonal antibodies	120:140	Biologically manufactured monoclonal antibodies (mAb)	94:146	Biologically manufactured monoclonal antibodies (mAb) can strongly vary in their efficacy and affinity.					
31260704	4	25	theme	day-by-day	722:731	arg1	variation					733:741	the day-by-day variation	718:741	the day-by-day variation of the terminal galactose within two major N-glycoforms	718:797	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	0	26	theme	glycosylation	23:35	arg1	pattern					37:43	antibody glycosylation pattern	14:43	antibody glycosylation pattern	14:43	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	8	27	theme	promising	1385:1393	arg1	tool					1406:1409	a promising analytical tool	1383:1409	a promising analytical tool for glycosylation control during the production process of recombinant proteins	1383:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	8	27	theme	promising	1385:1393	arg1	technology					1369:1378	the microarray technology	1354:1378	the microarray technology	1354:1378	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	1	28	theme	monoclonal	120:129	arg1	mAb					143:145	mAb	143:145	mAb	143:145	Biologically manufactured monoclonal antibodies (mAb) can strongly vary in their efficacy and affinity.					
31260704	1	28	theme	monoclonal	120:129	arg1	antibodies					131:140	monoclonal antibodies	120:140	Biologically manufactured monoclonal antibodies (mAb)	94:146	Biologically manufactured monoclonal antibodies (mAb) can strongly vary in their efficacy and affinity.					
31260704	0	29	theme	antibody	14:21	arg1	pattern					37:43	antibody glycosylation pattern	14:43	antibody glycosylation pattern	14:43	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	8	30	theme	production	1448:1457	arg1	process					1459:1465	the production process	1444:1465	the production process of recombinant proteins	1444:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	4	31	theme	terminal	750:757	arg1	galactose					759:767	the terminal galactose	746:767	the terminal galactose	746:767	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	3	32	theme	microarray	483:492	arg1	technology					494:503	a microarray technology	481:503	a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	481:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	33	theme	mass	436:439	arg1	MALDI-MS					455:462	MALDI-MS	455:462	MALDI-MS	455:462	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	33	theme	mass	436:439	arg1	spectrometry					441:452	matrix-assisted laser desorption/ionization mass spectrometry	392:452	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	4	34	theme	bottom-up	580:588	arg1	approach					590:597	A bottom-up approach	578:597	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification	578:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	7	35	theme	released	1231:1238	arg1	N-glycans					1240:1248	released N-glycans	1231:1248	released N-glycans	1231:1248	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	2	36	theme	mAb	243:245	arg1	production					225:234	production	225:234	production	225:234	Therefore, engineering and production of the mAb is highly regulated and requires product monitoring, especially in terms of N-glycosylation patterns.					
31260704	2	36	theme	mAb	243:245	arg1	engineering					209:219	engineering	209:219	engineering	209:219	Therefore, engineering and production of the mAb is highly regulated and requires product monitoring, especially in terms of N-glycosylation patterns.					
31260704	5	37	theme	distribution	933:944	arg1	determination					899:911	the rapid determination	889:911	the rapid determination of the carbohydrate distribution	889:944	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	5	38	theme	rapid	893:897	arg1	determination					899:911	the rapid determination	889:911	the rapid determination of the carbohydrate distribution	889:944	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	0	39	theme	pattern	37:43	arg1	Monitoring					0:9	Monitoring	0:9	Monitoring of antibody glycosylation pattern	0:43	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	6	40	theme	variations	1015:1024	arg1	coefficient					1000:1010	a low coefficient	994:1010	a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively)	994:1087	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	3	41	theme	spectrometry	441:452	arg1	method					465:470	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method	374:470	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	6	42	dep	variations	1015:1024	arg1	%					1030:1030	1.7%	1027:1030	1.7%	1027:1030	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	6	42	dep	variations	1015:1024	arg1	%					1039:1039	7.1%	1036:1039	7.1% for the FA2 and FA2G1 structures	1036:1072	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	5	43	theme	robust	869:874	arg1	microarrays					822:832	microarrays	822:832	microarrays for mass spectrometry (MAMS)	822:861	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	5	43	theme	robust	869:874	arg1	platform					876:883	a robust platform	867:883	a robust platform for the rapid determination of the carbohydrate distribution	867:944	Our results show that microarrays for mass spectrometry (MAMS) are a robust platform for the rapid determination of the carbohydrate distribution.					
31260704	3	44	theme	laser	408:412	arg1	MALDI-MS					455:462	MALDI-MS	455:462	MALDI-MS	455:462	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	44	theme	laser	408:412	arg1	spectrometry					441:452	matrix-assisted laser desorption/ionization mass spectrometry	392:452	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	0	45	theme	MALDI-TOF	65:73	arg1	spectrometry					80:91	microarray MALDI-TOF mass spectrometry	54:91	microarray MALDI-TOF mass spectrometry	54:91	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	3	46	theme	desorption/ionization	414:434	arg1	MALDI-MS					455:462	MALDI-MS	455:462	MALDI-MS	455:462	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	46	theme	desorption/ionization	414:434	arg1	spectrometry					441:452	matrix-assisted laser desorption/ionization mass spectrometry	392:452	a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture	374:575	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	6	47	theme	low	996:998	arg1	coefficient					1000:1010	a low coefficient	994:1010	a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively)	994:1087	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	8	48	theme	microarray	1358:1367	arg1	tool					1406:1409	a promising analytical tool	1383:1409	a promising analytical tool for glycosylation control during the production process of recombinant proteins	1383:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	8	48	theme	microarray	1358:1367	arg1	technology					1369:1378	the microarray technology	1354:1378	the microarray technology	1354:1378	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	6	49	theme	FA2G1	1057:1061	arg1	structures					1063:1072	the FA2 and FA2G1 structures	1045:1072	structures	1063:1072	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	2	50	theme	patterns	339:346	arg1	terms					314:318	terms	314:318	terms of N-glycosylation patterns	314:346	Therefore, engineering and production of the mAb is highly regulated and requires product monitoring, especially in terms of N-glycosylation patterns.					
31260704	6	51	theme	operating	1157:1165	arg1	conditions					1167:1176	operating conditions	1157:1176	operating conditions	1157:1176	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
31260704	4	52	used	used	700:703	arg2	approach					590:597	A bottom-up approach	578:597	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification	578:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	2	53	theme	N-glycosylation	323:337	arg1	patterns					339:346	N-glycosylation patterns	323:346	N-glycosylation patterns	323:346	Therefore, engineering and production of the mAb is highly regulated and requires product monitoring, especially in terms of N-glycosylation patterns.					
31260704	4	54	theme	liquid	650:655	arg1	chromatography					657:670	zwitterionic-hydrophilic interaction liquid chromatography	613:670	zwitterionic-hydrophilic interaction liquid chromatography for sample purification	613:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	0	55	theme	mass	75:78	arg1	spectrometry					80:91	microarray MALDI-TOF mass spectrometry	54:91	microarray MALDI-TOF mass spectrometry	54:91	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	3	56	attach	present	366:372	arg2	we					363:364	we	363:364	we	363:364	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	3	56	attach	present	366:372	arg1	work					357:360	this work	352:360	this work	352:360	In this work, we present a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method based on a microarray technology to monitor N-glycopeptides of IgG1 produced in a perfusion cell culture.					
31260704	7	57	with	electrophoresis	1284:1298	arg1	detection					1332:1340	laser-induced fluorescence detection	1305:1340	laser-induced fluorescence detection	1305:1340	The observed trend of released N-glycans was confirmed using capillary gel electrophoresis with laser-induced fluorescence detection.					
31260704	8	58	theme	proteins	1482:1489	arg1	process					1459:1465	the production process	1444:1465	the production process of recombinant proteins	1444:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	4	59	theme	sample	676:681	arg1	purification					683:694	sample purification	676:694	sample purification	676:694	A bottom-up approach combined with zwitterionic-hydrophilic interaction liquid chromatography for sample purification was used to determine the day-by-day variation of the terminal galactose within two major N-glycoforms.					
31260704	0	60	theme	microarray	54:63	arg1	spectrometry					80:91	microarray MALDI-TOF mass spectrometry	54:91	microarray MALDI-TOF mass spectrometry	54:91	Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.					
31260704	8	61	theme	analytical	1395:1404	arg1	tool					1406:1409	a promising analytical tool	1383:1409	a promising analytical tool for glycosylation control during the production process of recombinant proteins	1383:1489	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	8	61	theme	analytical	1395:1404	arg1	technology					1369:1378	the microarray technology	1354:1378	the microarray technology	1354:1378	Therefore, the microarray technology is a promising analytical tool for glycosylation control during the production process of recombinant proteins.					
31260704	6	62	theme	FA2	1049:1051	arg1	structures					1063:1072	the FA2 and FA2G1 structures	1045:1072	structures	1063:1072	The spectral repeatability is characterized by a low coefficient of variations (1.7% and 7.1% for the FA2 and FA2G1 structures, respectively) and allows to detect the N-glycosylation variability resulting from operating conditions during the bioreactor process.					
30294837	8	0	theme	aglycon	1500:1506	arg1	value					1487:1491	the pKa value	1479:1491	the pKa value	1479:1491	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	8	0	theme	aglycon	1500:1506	arg1	yield					1469:1473	the yield	1465:1473	the yield	1465:1473	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	7	1	dep	%	1215:1215	arg1	v/v					1218:1220	v/v	1218:1220	v/v	1218:1220	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	2	2	theme	anti-inflammatory	375:391	arg1	activities					408:417	anti-inflammatory and anticancer activities	375:417	anti-inflammatory and anticancer activities	375:417	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	7	3	theme	2-h	1154:1156	arg1	process					1158:1164	a 2-h process	1152:1164	a 2-h process at pH 5.0 and 30 °C	1152:1184	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	6	4	theme	glycosylated	998:1009	arg1	NMR					975:977	NMR	975:977	NMR	975:977	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	6	4	theme	glycosylated	998:1009	arg1	product					1011:1017	a single glycosylated product	989:1017	a single glycosylated product with a free hydroxyl group was formed	989:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	3	5	theme	7-O-rutinosylated	575:591	arg1	flavonoids					593:602	7-O-rutinosylated flavonoids	575:602	7-O-rutinosylated flavonoids	575:602	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	5	6	theme	alkaline	816:823	arg1	environment					833:843	alkaline aqueous environment	816:843	alkaline aqueous environment	816:843	Since HQ undergoes oxidation in a neutral to alkaline aqueous environment, the transglycosylation process was carried out at pH values ≤6.0.					
30294837	6	7	theme	single	991:996	arg1	NMR					975:977	NMR	975:977	NMR	975:977	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	6	7	theme	single	991:996	arg1	product					1011:1017	a single glycosylated product	989:1017	a single glycosylated product with a free hydroxyl group was formed	989:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	2	8	theme	pharmacokinetic	306:320	arg1	properties					322:331	physicochemical and pharmacokinetic properties	286:331	physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities	286:417	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	6	9	gly	glycosylated	998:1009	arg1	NMR					975:977	NMR	975:977	NMR	975:977	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	6	9	gly	glycosylated	998:1009	arg1	product					1011:1017	a single glycosylated product	989:1017	a single glycosylated product with a free hydroxyl group was formed	989:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	5	10	theme	aqueous	825:831	arg1	environment					833:843	alkaline aqueous environment	816:843	alkaline aqueous environment	816:843	Since HQ undergoes oxidation in a neutral to alkaline aqueous environment, the transglycosylation process was carried out at pH values ≤6.0.					
30294837	3	11	from	flavonoids	593:602	arg1	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose					527:567	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose	527:567	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose	527:567	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	11	from	flavonoids	593:602	arg1	moiety					519:524	the rutinosyl moiety	505:524	the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds	505:636	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	8	12	dep	compounds	1320:1328	arg1	resorcinol					1347:1356	resorcinol	1347:1356	resorcinol	1347:1356	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	8	12	dep	compounds	1320:1328	arg1	phloroglucinol					1331:1344	phloroglucinol	1331:1344	phloroglucinol	1331:1344	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	8	12	dep	compounds	1320:1328	arg1	catechol					1371:1378	catechol	1371:1378	catechol	1371:1378	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	8	12	dep	compounds	1320:1328	arg1	pyrogallol					1359:1368	pyrogallol	1359:1368	pyrogallol	1359:1368	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	6	13	with	product	1011:1017	arg1	group					1040:1044	a free hydroxyl group	1024:1044	a free hydroxyl group was formed	1024:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	8	14	theme	various	1303:1309	arg1	compounds					1320:1328	various phenolic compounds	1303:1328	various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol)	1303:1379	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	2	15	theme	physicochemical	286:300	arg1	properties					322:331	physicochemical and pharmacokinetic properties	286:331	physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities	286:417	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	0	16	theme	Acremonium	123:132	arg1	sp					134:135	Acremonium sp	123:135	Acremonium sp	123:135	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	9	17	theme	phenolic	1627:1634	arg1	compounds					1636:1644	small phenolic compounds	1621:1644	small phenolic compounds	1621:1644	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	3	18	theme	enzyme	424:429	arg1	EC					462:463	EC 3.2.1.168	462:473	EC 3.2.1.168	462:473	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	18	theme	enzyme	424:429	arg1	6-O-α-rhamnosyl-β-glucosidase					431:459	The enzyme 6-O-α-rhamnosyl-β-glucosidase	420:459	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168)	420:474	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	8	19	theme	pKa	1483:1485	arg1	value					1487:1491	the pKa value	1479:1491	the pKa value	1479:1491	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	5	20	theme	transglycosylation	850:867	arg1	process					869:875	the transglycosylation process	846:875	the transglycosylation process	846:875	Since HQ undergoes oxidation in a neutral to alkaline aqueous environment, the transglycosylation process was carried out at pH values ≤6.0.					
30294837	8	21	theme	direct	1430:1435	arg1	relationship					1444:1455	an apparent direct linear relationship	1418:1455	an apparent direct linear relationship between the yield and the pKa value of the aglycon	1418:1506	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	9	22	theme	convenient	1559:1568	arg1	processes					1586:1594	convenient and sustainable processes	1559:1594	convenient and sustainable processes	1559:1594	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	2	23	theme	entire	340:345	arg1	compound					347:354	the entire compound	336:354	the entire compound	336:354	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	0	24	theme	Enzyme-mediated	0:14	arg1	6-O-α-l-rhamnosyl-d-glucose					48:74	6-O-α-l-rhamnosyl-d-glucose	48:74	6-O-α-l-rhamnosyl-d-glucose	48:74	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	0	24	theme	Enzyme-mediated	0:14	arg1	transglycosylation					16:33	Enzyme-mediated transglycosylation	0:33	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.	0:136	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	4	25	theme	transglycosylation	644:661	arg1	reaction					663:670	This transglycosylation reaction	639:670	This transglycosylation reaction	639:670	This transglycosylation reaction was optimized using hydroquinone (HQ) and hesperidin as rutinose acceptor and donor, respectively.					
30294837	9	26	theme	compounds	1636:1644	arg1	glycosylation					1604:1616	the glycosylation	1600:1616	the glycosylation of small phenolic compounds	1600:1644	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	0	27	from	sp	134:135	arg1	diglycosidase					104:116	a diglycosidase	102:116	a diglycosidase from Acremonium sp	102:135	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	0	28	theme	rutinose	38:45	arg1	6-O-α-l-rhamnosyl-d-glucose					48:74	6-O-α-l-rhamnosyl-d-glucose	48:74	6-O-α-l-rhamnosyl-d-glucose	48:74	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	0	28	theme	rutinose	38:45	arg1	transglycosylation					16:33	Enzyme-mediated transglycosylation	0:33	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.	0:136	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	9	29	theme	small	1621:1625	arg1	compounds					1636:1644	small phenolic compounds	1621:1644	small phenolic compounds	1621:1644	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	9	30	theme	processes	1586:1594	arg1	development					1544:1554	the development	1540:1554	the development of convenient and sustainable processes	1540:1594	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	4	31	theme	rutinose	728:735	arg1	acceptor					737:744	rutinose acceptor	728:744	rutinose acceptor	728:744	This transglycosylation reaction was optimized using hydroquinone (HQ) and hesperidin as rutinose acceptor and donor, respectively.					
30294837	9	32	theme	sustainable	1574:1584	arg1	processes					1586:1594	convenient and sustainable processes	1559:1594	convenient and sustainable processes	1559:1594	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	7	33	from	pH	1169:1170	arg1	process					1158:1164	a 2-h process	1152:1164	a 2-h process at pH 5.0 and 30 °C	1152:1184	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	3	34	theme	hydroxylated	607:618	arg1	compounds					628:636	hydroxylated organic compounds	607:636	hydroxylated organic compounds	607:636	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	35	theme	rutinosyl	509:517	arg1	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose					527:567	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose	527:567	6-O-α-l-rhamnopyranosyl-β-d-glucopyranose	527:567	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	35	theme	rutinosyl	509:517	arg1	moiety					519:524	the rutinosyl moiety	505:524	the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds	505:636	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	7	36	dep	4-hydroxyphenyl-β-rutinoside	1079:1106	arg1	%					1111:1111	38%	1109:1111	38%	1109:1111	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	7	36	dep	4-hydroxyphenyl-β-rutinoside	1079:1106	arg1	hesperidin					1124:1133	regarding hesperidin	1114:1133	regarding hesperidin	1114:1133	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	7	37	theme	5	1214:1214	arg1	%					1215:1215	%	1215:1215	%	1215:1215	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	8	38	theme	same	1244:1247	arg1	conditions					1249:1258	the same conditions	1240:1258	the same conditions	1240:1258	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	2	39	theme	moiety	183:188	arg1	structure					153:161	The structure	149:161	The structure of the carbohydrate moiety of a natural phenolic glycoside	149:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	0	40	gly	transglycosylation	16:33	arg1	rutinose					38:45	rutinose	38:45	rutinose	38:45	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	8	41	theme	compounds	1320:1328	arg1	glycoconjugates					1284:1298	glycoconjugates	1284:1298	glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol)	1284:1379	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	7	42	theme	36 mM	1192:1196	arg1	OH-acceptor					1198:1208	36 mM OH-acceptor	1192:1208	36 mM OH-acceptor	1192:1208	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	6	43	theme	hydroxyl	1031:1038	arg1	group					1040:1044	a free hydroxyl group	1024:1044	a free hydroxyl group was formed	1024:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	8	44	theme	phenolic	1311:1318	arg1	compounds					1320:1328	various phenolic compounds	1303:1328	various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol)	1303:1379	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	2	45	theme	carbohydrate	170:181	arg1	moiety					183:188	the carbohydrate moiety	166:188	the carbohydrate moiety of a natural phenolic glycoside	166:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	8	46	theme	apparent	1421:1428	arg1	relationship					1444:1455	an apparent direct linear relationship	1418:1455	an apparent direct linear relationship between the yield and the pKa value of the aglycon	1418:1506	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	5	47	theme	pH	896:897	arg1	values					899:904	pH values ≤6.0	896:909	pH values ≤6.0	896:909	Since HQ undergoes oxidation in a neutral to alkaline aqueous environment, the transglycosylation process was carried out at pH values ≤6.0.					
30294837	6	48	theme	free	1026:1029	arg1	group					1040:1044	a free hydroxyl group	1024:1044	a free hydroxyl group was formed	1024:1055	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	3	49	contain	has	476:478	arg1	EC					462:463	EC 3.2.1.168	462:473	EC 3.2.1.168	462:473	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	49	contain	has	476:478	arg2	capacity					484:491	the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds	480:636	the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds	480:636	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	3	49	contain	has	476:478	arg1	6-O-α-rhamnosyl-β-glucosidase					431:459	The enzyme 6-O-α-rhamnosyl-β-glucosidase	420:459	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168)	420:474	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	6	50	theme	4-hydroxyphenyl-β-rutinoside	929:956	arg1	structure					916:924	The structure	912:924	The structure of 4-hydroxyphenyl-β-rutinoside	912:956	The structure of 4-hydroxyphenyl-β-rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed.					
30294837	1	51	theme	DSM	138:140	arg1	24697					142:146	DSM 24697	138:146	DSM 24697.	138:147	DSM 24697.					
30294837	7	52	theme	%	1215:1215	arg1	cosolvent					1223:1231	5% (v/v) cosolvent	1214:1231	5% (v/v) cosolvent	1214:1231	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	7	53	theme	regarding	1114:1122	arg1	%					1111:1111	38%	1109:1111	38%	1109:1111	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	7	53	theme	regarding	1114:1122	arg1	hesperidin					1124:1133	regarding hesperidin	1114:1133	regarding hesperidin	1114:1133	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	2	54	theme	glycoside	212:220	arg1	moiety					183:188	the carbohydrate moiety	166:188	the carbohydrate moiety of a natural phenolic glycoside	166:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	8	55	theme	linear	1437:1442	arg1	relationship					1444:1455	an apparent direct linear relationship	1418:1455	an apparent direct linear relationship between the yield and the pKa value of the aglycon	1418:1506	Under the same conditions, the enzyme synthesized glycoconjugates of various phenolic compounds (phloroglucinol, resorcinol, pyrogallol, catechol), with yields between 12% and 28% and an apparent direct linear relationship between the yield and the pKa value of the aglycon.					
30294837	2	56	theme	significant	233:243	arg1	effect					245:250	a significant effect	231:250	a significant effect	231:250	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	57	contain	have	226:229	arg1	structure					153:161	The structure	149:161	The structure of the carbohydrate moiety of a natural phenolic glycoside	149:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	57	contain	have	226:229	arg2	effect					245:250	a significant effect	231:250	a significant effect	231:250	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	3	58	theme	organic	620:626	arg1	compounds					628:636	hydroxylated organic compounds	607:636	hydroxylated organic compounds	607:636	The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2.1.168) has the capacity to transfer the rutinosyl moiety (6-O-α-l-rhamnopyranosyl-β-d-glucopyranose) from 7-O-rutinosylated flavonoids to hydroxylated organic compounds.					
30294837	2	59	theme	phenolic	203:210	arg1	glycoside					212:220	a natural phenolic glycoside	193:220	a natural phenolic glycoside	193:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	60	theme	molecular	259:267	arg1	interactions					269:280	the molecular interactions	255:280	the molecular interactions	255:280	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	61	theme	compound	347:354	arg1	properties					322:331	physicochemical and pharmacokinetic properties	286:331	physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities	286:417	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	61	theme	compound	347:354	arg1	interactions					269:280	the molecular interactions	255:280	the molecular interactions	255:280	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	2	62	theme	natural	195:201	arg1	glycoside					212:220	a natural phenolic glycoside	193:220	a natural phenolic glycoside	193:220	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	7	63	theme	4-hydroxyphenyl-β-rutinoside	1079:1106	arg1	yield					1070:1074	The highest yield	1058:1074	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin)	1058:1134	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	2	64	theme	anticancer	397:406	arg1	activities					408:417	anti-inflammatory and anticancer activities	375:417	anti-inflammatory and anticancer activities	375:417	The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities.					
30294837	0	65	theme	phenolic	80:87	arg1	compounds					89:97	phenolic compounds	80:97	phenolic compounds	80:97	Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp.					
30294837	7	66	theme	highest	1062:1068	arg1	yield					1070:1074	The highest yield	1058:1074	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin)	1058:1134	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
30294837	9	67	gly	glycosylation	1604:1616	arg1	compounds					1636:1644	small phenolic compounds	1621:1644	small phenolic compounds	1621:1644	This work is a contribution to the development of convenient and sustainable processes for the glycosylation of small phenolic compounds.					
30294837	7	68	from	30 °C	1180:1184	arg1	process					1158:1164	a 2-h process	1152:1164	a 2-h process at pH 5.0 and 30 °C	1152:1184	The highest yield of 4-hydroxyphenyl-β-rutinoside (38%, regarding hesperidin) was achieved in a 2-h process at pH 5.0 and 30 °C, with 36 mM OH-acceptor and 5% (v/v) cosolvent.					
29632092	6	0	dep	structure	1825:1833	arg1	the					1821:1823	the	1821:1823	the	1821:1823	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	8	1	theme	glycosylation	2196:2208	arg1	pathways					2210:2217	glycosylation pathways	2196:2217	glycosylation pathways	2196:2217	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	4	2	theme	outside	1045:1051	arg1	surface					1053:1059	the outside surface	1041:1059	the outside surface of the cell (GtcA; SAOUHSC_02722)	1041:1093	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	3	theme	environmental	1666:1678	arg1	media					1733:1737	growth media	1726:1737	growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1726:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	3	theme	environmental	1666:1678	arg1	conditions					1704:1713	environmental stress-inducing culture conditions	1666:1713	environmental stress-inducing culture conditions	1666:1713	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	4	theme	GlcNAcylated	1425:1436	arg1	LTA					1438:1440	GlcNAcylated LTA	1425:1440	GlcNAcylated LTA under normal growth conditions	1425:1471	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	5	theme	undecaprenol	901:912	arg1	GlcNAc					941:946	GlcNAc	941:946	GlcNAc	941:946	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	5	theme	undecaprenol	901:912	arg1	N-acetylglucosamine					920:938	an undecaprenol (Und) N-acetylglucosamine	898:938	an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	898:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	7	6	theme	sigma	2053:2057	arg1	SigB					2066:2069	the alternative sigma factor SigB	2037:2069	the alternative sigma factor SigB	2037:2069	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	4	7	theme	three-component	849:863	arg1	system					879:884	a membrane-associated three-component glycosylation system	827:884	a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	827:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	2	8	theme	cell	285:288	arg1	physiology					299:308	cell envelope physiology	285:308	cell envelope physiology	285:308	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	6	9	theme	overexpressing	1505:1518	arg1	CsbB					1520:1523	either overexpressing CsbB	1498:1523	either overexpressing CsbB	1498:1523	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	10	theme	LTA-specific	1111:1122	arg1	glycosyltransferase					1124:1142	an LTA-specific glycosyltransferase	1108:1142	an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	1108:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	2	11	theme	cell	332:335	arg1	autolysis					337:345	cell autolysis	332:345	cell autolysis	332:345	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	4	12	from	role	657:660	arg1	physiology					679:688	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	13	theme	culture	1696:1702	arg1	media					1733:1737	growth media	1726:1737	growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1726:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	13	theme	culture	1696:1702	arg1	conditions					1704:1713	environmental stress-inducing culture conditions	1666:1713	environmental stress-inducing culture conditions	1666:1713	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	14	theme	cell	665:668	arg1	physiology					679:688	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	15	theme	cell	1585:1588	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	7	16	theme	pathway	1985:1991	arg1	components					1933:1942	key components	1929:1942	key components of the LTA three-component glycosylation pathway	1929:1991	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	4	17	theme	Und	915:917	arg1	GlcNAc					941:946	GlcNAc	941:946	GlcNAc	941:946	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	17	theme	Und	915:917	arg1	N-acetylglucosamine					920:938	an undecaprenol (Und) N-acetylglucosamine	898:938	an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	898:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	0	18	from	System	90:95	arg1	aureus					115:120	Staphylococcus aureus	100:120	Staphylococcus aureus	100:120	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	8	19	theme	glycosylation	2270:2282	arg1	results					2284:2290	glycosylation results	2270:2290	glycosylation results	2270:2290	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	4	20	from	levels	736:741	arg1	aureus					776:781	S. aureus	773:781	S. aureus	773:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	21	from	role	1796:1799	arg1	function					1839:1846	function	1839:1846	function	1839:1846	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	21	from	role	1796:1799	arg1	structure					1825:1833	structure	1825:1833	structure	1825:1833	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	22	theme	low	732:734	arg1	levels					736:741	the low levels	728:741	the low levels of basal LTA glycosylation in S. aureus	728:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	7	23	theme	environmental	2135:2147	arg1	stresses					2149:2156	environmental stresses	2135:2156	environmental stresses	2135:2156	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	5	24	theme	cross	1262:1266	arg1	recognition					1268:1278	no cross recognition	1259:1278	no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids	1259:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	6	25	theme	endogenous	1535:1544	arg1	activity					1560:1567	endogenous d-alanylation activity	1535:1567	endogenous d-alanylation activity	1535:1567	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	3	26	mod	modified	446:453	arg3	groups					507:512	glycosyl groups	498:512	glycosyl groups	498:512	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	3	26	mod	modified	446:453	arg3	substituents					460:471	substituents	460:471	substituents	460:471	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	3	26	mod	modified	446:453	arg1	LTA					425:427	LTA	425:427	LTA	425:427	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	3	26	mod	modified	446:453	arg3	d-alanine					484:492	d-alanine	484:492	d-alanine	484:492	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	8	27	from	LTA	2249:2251	arg1	d-alanylation					2305:2317	decreased d-alanylation	2295:2317	decreased d-alanylation	2295:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	4	28	theme	α-GlcNAc	1154:1161	arg1	moieties					1163:1170	α-GlcNAc moieties	1154:1170	α-GlcNAc moieties	1154:1170	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	7	29	theme	tailoring	2104:2112	arg1	role					2076:2079	the role	2072:2079	the role of N-acetylglucosamine tailoring during adaptation to environmental stresses	2072:2156	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	7	30	theme	glycosylation	1971:1983	arg1	pathway					1985:1991	the LTA three-component glycosylation pathway	1947:1991	the LTA three-component glycosylation pathway	1947:1991	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	4	31	from	aureus	776:781	arg1	levels					736:741	the low levels	728:741	the low levels of basal LTA glycosylation in S. aureus	728:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	1	32	theme	poly-glycerophosphate	177:197	arg1	polymer					199:205	a poly-glycerophosphate polymer	175:205	a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane	175:256	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	1	32	theme	poly-glycerophosphate	177:197	arg1	acid					136:139	Lipoteichoic acid	123:139	Lipoteichoic acid (LTA) in Staphylococcus aureus	123:170	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	8	33	from	sites	2240:2244	arg1	induction					2257:2265	induction	2257:2265	induction of glycosylation results in decreased d-alanylation	2257:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	8	33	from	sites	2240:2244	arg1	LTA					2249:2251	LTA	2249:2251	LTA	2249:2251	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	7	34	theme	LTA	1951:1953	arg1	pathway					1985:1991	the LTA three-component glycosylation pathway	1947:1991	the LTA three-component glycosylation pathway	1947:1991	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	6	35	theme	factor	1635:1640	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	36	dep	levels	1755:1760	arg1	unknown					1908:1914	unknown	1908:1914	unknown	1908:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	36	dep	levels	1755:1760	arg1	role					1796:1799	The role	1792:1799	The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA)	1792:1895	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	37	theme	growth	1726:1731	arg1	media					1733:1737	growth media	1726:1737	growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1726:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	5	38	theme	polyribitol	1308:1318	arg1	phosphate					1320:1328	the structurally similar polyribitol phosphate	1283:1328	the structurally similar polyribitol phosphate containing wall teichoic acids	1283:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	5	39	with	LTA	1250:1252	arg1	recognition					1268:1278	no cross recognition	1259:1278	no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids	1259:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	6	40	theme	alternative	1617:1627	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	8	41	from	induction	2257:2265	arg1	d-alanylation					2305:2317	decreased d-alanylation	2295:2317	decreased d-alanylation	2295:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	2	42	theme	growth	406:411	arg1	conditions					413:422	environmental growth conditions	392:422	environmental growth conditions	392:422	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	0	43	theme	Salt-Induced	0:11	arg1	Stress					13:18	Salt-Induced Stress	0:18	Salt-Induced Stress	0:18	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	7	44	theme	key	1929:1931	arg1	components					1933:1942	key components	1929:1942	key components of the LTA three-component glycosylation pathway	1929:1991	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	6	45	theme	stress	1599:1604	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	1	46	theme	Lipoteichoic	123:134	arg1	LTA					142:144	LTA	142:144	LTA	142:144	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	1	46	theme	Lipoteichoic	123:134	arg1	acid					136:139	Lipoteichoic acid	123:139	Lipoteichoic acid (LTA) in Staphylococcus aureus	123:170	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	1	46	theme	Lipoteichoic	123:134	arg1	polymer					199:205	a poly-glycerophosphate polymer	175:205	a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane	175:256	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	4	47	theme	d-alanylation	577:589	arg1	determinants					561:572	the genetic determinants	549:572	the genetic determinants of d-alanylation	549:589	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	48	gly	glycosylation	1804:1816	arg1	LTA					1892:1894	LTA	1892:1894	LTA	1892:1894	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	48	gly	glycosylation	1804:1816	arg1	acid					1886:1889	Staphylococcus aureus lipoteichoic acid	1851:1889	Staphylococcus aureus lipoteichoic acid (LTA)	1851:1895	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	49	theme	LTA	752:754	arg1	glycosylation					756:768	basal LTA glycosylation	746:768	basal LTA glycosylation in S. aureus	746:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	50	theme	aureus	1394:1399	arg1	LTA					1401:1403	wild-type S. aureus LTA	1381:1403	wild-type S. aureus LTA	1381:1403	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	0	51	theme	Lipoteichoic	33:44	arg1	System					90:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System	31:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus	31:120	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	6	52	contain	has	1405:1407	arg2	trace					1416:1420	only a trace	1409:1420	only a trace of GlcNAcylated LTA under normal growth conditions	1409:1471	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	52	contain	has	1405:1407	arg1	LTA					1401:1403	wild-type S. aureus LTA	1381:1403	wild-type S. aureus LTA	1381:1403	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	7	53	theme	factor	2059:2064	arg1	SigB					2066:2069	the alternative sigma factor SigB	2037:2069	the alternative sigma factor SigB	2037:2069	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	0	54	theme	Three-Component	60:74	arg1	System					90:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System	31:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus	31:120	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	6	55	theme	sodium	1765:1770	arg1	chloride.IMPORTANCE					1772:1790	sodium chloride.IMPORTANCE	1765:1790	sodium chloride.IMPORTANCE	1765:1790	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	1	56	theme	cell	244:247	arg1	membrane					249:256	the cell membrane	240:256	the cell membrane	240:256	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	6	57	theme	lipoteichoic	1873:1884	arg1	LTA					1892:1894	LTA	1892:1894	LTA	1892:1894	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	57	theme	lipoteichoic	1873:1884	arg1	acid					1886:1889	Staphylococcus aureus lipoteichoic acid	1851:1889	Staphylococcus aureus lipoteichoic acid (LTA)	1851:1895	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	5	58	theme	teichoic	1346:1353	arg1	acids					1355:1359	wall teichoic acids	1341:1359	wall teichoic acids	1341:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	6	59	theme	normal	1448:1453	arg1	conditions					1462:1471	normal growth conditions	1448:1471	normal growth conditions	1448:1471	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	60	theme	S.	1391:1392	arg1	LTA					1401:1403	wild-type S. aureus LTA	1381:1403	wild-type S. aureus LTA	1381:1403	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	61	theme	glycosylation	635:647	arg1	route					622:626	the route	618:626	the route of LTA glycosylation	618:647	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	61	theme	glycosylation	635:647	arg1	role					657:660	its role	653:660	its role in cell envelope physiology	653:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	3	62	theme	cellular	524:531	arg1	function					533:540	cellular function	524:540	cellular function	524:540	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	6	63	theme	LTA	1438:1440	arg1	trace					1416:1420	only a trace	1409:1420	only a trace of GlcNAcylated LTA under normal growth conditions	1409:1471	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	2	64	contain	has	263:265	arg1	LTA					259:261	LTA	259:261	LTA	259:261	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	2	64	contain	has	263:265	arg2	roles					276:280	numerous roles	267:280	numerous roles	267:280	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	5	65	contain	containing	1330:1339	arg1	phosphate					1320:1328	the structurally similar polyribitol phosphate	1283:1328	the structurally similar polyribitol phosphate containing wall teichoic acids	1283:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	5	65	contain	containing	1330:1339	arg2	acids					1355:1359	wall teichoic acids	1341:1359	wall teichoic acids	1341:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	2	66	theme	envelope	290:297	arg1	physiology					299:308	cell envelope physiology	285:308	cell envelope physiology	285:308	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	6	67	theme	wild-type	1381:1389	arg1	LTA					1401:1403	wild-type S. aureus LTA	1381:1403	wild-type S. aureus LTA	1381:1403	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	8	68	theme	antimicrobial	2424:2436	arg1	peptides					2438:2445	antimicrobial peptides	2424:2445	antimicrobial peptides	2424:2445	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	4	69	theme	membrane-associated	829:847	arg1	system					879:884	a membrane-associated three-component glycosylation system	827:884	a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	827:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	70	theme	envelope	670:677	arg1	physiology					679:688	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	7	71	theme	alternative	2041:2051	arg1	SigB					2066:2069	the alternative sigma factor SigB	2037:2069	the alternative sigma factor SigB	2037:2069	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	4	72	theme	glycosylation	865:877	arg1	system					879:884	a membrane-associated three-component glycosylation system	827:884	a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213)	827:1199	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	8	73	theme	same	2235:2238	arg1	sites					2240:2244	the same sites	2231:2244	the same sites on LTA and induction of glycosylation results in decreased d-alanylation	2231:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	6	74	theme	envelope	1590:1597	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	1	75	theme	outer	223:227	arg1	surface					229:235	the outer surface	219:235	the outer surface of the cell membrane	219:256	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	7	76	theme	stress-induced	2008:2021	arg1	regulation					2023:2032	stress-induced regulation	2008:2032	stress-induced regulation by the alternative sigma factor SigB	2008:2069	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	6	77	theme	stress-inducing	1680:1694	arg1	media					1733:1737	growth media	1726:1737	growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1726:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	77	theme	stress-inducing	1680:1694	arg1	conditions					1704:1713	environmental stress-inducing culture conditions	1666:1713	environmental stress-inducing culture conditions	1666:1713	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	78	theme	due	721:723	arg1	part					716:719	part	716:719	part due to the low levels of basal LTA glycosylation in S. aureus	716:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	8	79	theme	results	2284:2290	arg1	induction					2257:2265	induction	2257:2265	induction of glycosylation results in decreased d-alanylation	2257:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	8	79	theme	results	2284:2290	arg1	LTA					2249:2251	LTA	2249:2251	LTA	2249:2251	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	4	80	gly	glycosylation	756:768	arg1	aureus					776:781	S. aureus	773:781	S. aureus	773:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	2	81	theme	numerous	267:274	arg1	roles					276:280	numerous roles	267:280	numerous roles	267:280	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	6	82	theme	d-alanylation	1546:1558	arg1	activity					1560:1567	endogenous d-alanylation activity	1535:1567	endogenous d-alanylation activity	1535:1567	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	8	83	theme	decreased	2295:2303	arg1	d-alanylation					2305:2317	decreased d-alanylation	2295:2317	decreased d-alanylation	2295:2317	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	6	84	theme	acid	1886:1889	arg1	function					1839:1846	function	1839:1846	function	1839:1846	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	84	theme	acid	1886:1889	arg1	structure					1825:1833	structure	1825:1833	structure	1825:1833	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	7	85	theme	three-component	1955:1969	arg1	pathway					1985:1991	the LTA three-component glycosylation pathway	1947:1991	the LTA three-component glycosylation pathway	1947:1991	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	6	86	theme	high	1750:1753	arg1	levels					1755:1760	high levels	1750:1760	high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1750:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	87	from	route	622:626	arg1	physiology					679:688	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	88	gly	glycosylation	635:647	arg1	cell					665:668	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	88	gly	glycosylation	635:647	arg1	envelope					670:677	cell envelope physiology	665:688	cell envelope physiology	665:688	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	89	theme	glycosylation	756:768	arg1	levels					736:741	the low levels	728:741	the low levels of basal LTA glycosylation in S. aureus	728:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	5	90	theme	similar	1300:1306	arg1	phosphate					1320:1328	the structurally similar polyribitol phosphate	1283:1328	the structurally similar polyribitol phosphate containing wall teichoic acids	1283:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	4	91	from	glycosylation	756:768	arg1	aureus					776:781	S. aureus	773:781	S. aureus	773:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	6	92	contain	containing	1739:1748	arg1	media					1733:1737	growth media	1726:1737	growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1726:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	92	contain	containing	1739:1748	arg2	levels					1755:1760	high levels	1750:1760	high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1750:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	5	93	theme	phosphate	1320:1328	arg1	recognition					1268:1278	no cross recognition	1259:1278	no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids	1259:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	6	94	theme	responsive	1606:1615	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	2	95	theme	environmental	392:404	arg1	conditions					413:422	environmental growth conditions	392:422	environmental growth conditions	392:422	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	6	96	theme	sigma	1629:1633	arg1	SigB					1642:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	the cell envelope stress responsive alternative sigma factor SigB	1581:1645	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	97	theme	glycosylation	1804:1816	arg1	unknown					1908:1914	unknown	1908:1914	unknown	1908:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	97	theme	glycosylation	1804:1816	arg1	role					1796:1799	The role	1792:1799	The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA)	1792:1895	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	4	98	theme	basal	746:750	arg1	glycosylation					756:768	basal LTA glycosylation	746:768	basal LTA glycosylation in S. aureus	746:781	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	3	99	theme	glycosyl	498:505	arg1	groups					507:512	glycosyl groups	498:512	glycosyl groups	498:512	LTA is often further modified with substituents, including d-alanine and glycosyl groups, to alter cellular function.					
29632092	0	100	theme	Acid-Specific	46:58	arg1	System					90:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System	31:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus	31:120	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	4	101	theme	putative	990:997	arg1	flippase					999:1006	a putative flippase	988:1006	a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722)	988:1093	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	102	theme	genetic	553:559	arg1	determinants					561:572	the genetic determinants	549:572	the genetic determinants of d-alanylation	549:589	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	0	103	theme	Glycosylation	76:88	arg1	System					90:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System	31:95	a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus	31:120	Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.					
29632092	7	104	theme	N-acetylglucosamine	2084:2102	arg1	tailoring					2104:2112	N-acetylglucosamine tailoring	2084:2112	N-acetylglucosamine tailoring	2084:2112	By defining key components of the LTA three-component glycosylation pathway and uncovering stress-induced regulation by the alternative sigma factor SigB, the role of N-acetylglucosamine tailoring during adaptation to environmental stresses can now be elucidated.					
29632092	6	105	theme	chloride.IMPORTANCE	1772:1790	arg1	levels					1755:1760	high levels	1750:1760	high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown	1750:1914	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	2	106	theme	cell	361:364	arg1	division					366:373	cell division	361:373	cell division	361:373	LTA has numerous roles in cell envelope physiology, including regulating cell autolysis, coordinating cell division, and adapting to environmental growth conditions.					
29632092	1	107	from	acid	136:139	arg1	aureus					165:170	Staphylococcus aureus	150:170	Staphylococcus aureus	150:170	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	4	108	theme	cell	1068:1071	arg1	surface					1053:1059	the outside surface	1041:1059	the outside surface of the cell (GtcA; SAOUHSC_02722)	1041:1093	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	4	109	theme	LTA	631:633	arg1	glycosylation					635:647	LTA glycosylation	631:647	LTA glycosylation	631:647	While the genetic determinants of d-alanylation have been largely defined, the route of LTA glycosylation and its role in cell envelope physiology have remained unknown, in part due to the low levels of basal LTA glycosylation in S. aureus We demonstrate here that S. aureus utilizes a membrane-associated three-component glycosylation system composed of an undecaprenol (Und) N-acetylglucosamine (GlcNAc) charging enzyme (CsbB; SAOUHSC_00713), a putative flippase to transport loaded substrate to the outside surface of the cell (GtcA; SAOUHSC_02722), and finally an LTA-specific glycosyltransferase that adds α-GlcNAc moieties to LTA (YfhO; SAOUHSC_01213).					
29632092	5	110	theme	wall	1341:1344	arg1	acids					1355:1359	wall teichoic acids	1341:1359	wall teichoic acids	1341:1359	We demonstrate that this system is specific for LTA with no cross recognition of the structurally similar polyribitol phosphate containing wall teichoic acids.					
29632092	6	111	theme	growth	1455:1460	arg1	conditions					1462:1471	normal growth conditions	1448:1471	normal growth conditions	1448:1471	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	1	112	theme	membrane	249:256	arg1	surface					229:235	the outer surface	219:235	the outer surface of the cell membrane	219:256	Lipoteichoic acid (LTA) in Staphylococcus aureus is a poly-glycerophosphate polymer anchored to the outer surface of the cell membrane.					
29632092	8	113	theme	modification	2350:2361	arg1	systems					2363:2369	the two modification systems	2342:2369	the two modification systems	2342:2369	As the dlt and glycosylation pathways compete for the same sites on LTA and induction of glycosylation results in decreased d-alanylation, the interplay between the two modification systems holds implications for resistance to antibiotics and antimicrobial peptides.					
29632092	6	114	theme	aureus	1866:1871	arg1	LTA					1892:1894	LTA	1892:1894	LTA	1892:1894	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
29632092	6	114	theme	aureus	1866:1871	arg1	acid					1886:1889	Staphylococcus aureus lipoteichoic acid	1851:1889	Staphylococcus aureus lipoteichoic acid (LTA)	1851:1895	We show that while wild-type S. aureus LTA has only a trace of GlcNAcylated LTA under normal growth conditions, amounts are raised upon either overexpressing CsbB, reducing endogenous d-alanylation activity, expressing the cell envelope stress responsive alternative sigma factor SigB, or by exposure to environmental stress-inducing culture conditions, including growth media containing high levels of sodium chloride.IMPORTANCE The role of glycosylation in the structure and function of Staphylococcus aureus lipoteichoic acid (LTA) is largely unknown.					
30230814	5	0	theme	2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose	835:887	arg1	synthesis					753:761	a synthesis	751:761	a synthesis	751:761	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	7	1	theme	Pse	1243:1245	arg1	biosynthesis					1247:1258	Pse biosynthesis	1243:1258	Pse biosynthesis	1243:1258	Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.					
30230814	1	2	from	rise	85:88	arg1	bacteria					114:121	antibiotic-resistant bacteria	93:121	antibiotic-resistant bacteria	93:121	The rise in antibiotic-resistant bacteria is causing worldwide concerns.					
30230814	7	3	theme	useful	1197:1202	arg1	assay					1204:1208	a useful assay	1195:1208	a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway	1195:1303	Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.					
30230814	0	4	from	Labeling	10:17	arg1	Surfaces					71:78	Bacterial Surfaces	61:78	Bacterial Surfaces	61:78	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	7	5	theme	biosynthesis	1247:1258	arg1	presence					1231:1238	phenotypic presence	1220:1238	phenotypic presence of Pse biosynthesis	1220:1258	Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.					
30230814	6	6	theme	vulnificus	1108:1117	arg1	strains					1148:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains.					
30230814	5	7	theme	2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose	766:818	arg1	synthesis					753:761	a synthesis	751:761	a synthesis	751:761	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	2	8	dep	drugs	192:196	arg1	calls					198:202	calls	198:202	new antibacterial drugs calls	174:202	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	5	9	theme	effective	935:943	arg1	Alt-4NAz					913:920	Alt-4NAz	913:920	Alt-4NAz	913:920	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	5	9	theme	effective	935:943	arg1	reporter					954:961	an effective chemical reporter	932:961	an effective chemical reporter to realize bacterial Pse metabolic labeling	932:1005	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	2	10	theme	antibacterial	293:305	arg1	targets					307:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	5	11	theme	bacterial	974:982	arg1	labeling					998:1005	bacterial Pse metabolic labeling	974:1005	bacterial Pse metabolic labeling	974:1005	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	2	12	theme	urgent	158:163	arg1	need					165:168	The urgent need	154:168	The urgent need for new antibacterial drugs calls for new thinking and strategies	154:234	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	7	13	theme	phenotypic	1220:1229	arg1	presence					1231:1238	phenotypic presence	1220:1238	phenotypic presence of Pse biosynthesis	1220:1258	Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.					
30230814	2	14	theme	pathogen-specific	275:291	arg1	targets					307:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	3	15	theme	sialic	443:448	arg1	acid					450:453	eukaryotic sialic acid	432:453	eukaryotic sialic acid	432:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	0	16	theme	Metabolic	0:8	arg1	Labeling					10:17	Metabolic Labeling	0:17	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces	0:78	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	1	17	theme	antibiotic-resistant	93:112	arg1	bacteria					114:121	antibiotic-resistant bacteria	93:121	antibiotic-resistant bacteria	93:121	The rise in antibiotic-resistant bacteria is causing worldwide concerns.					
30230814	5	18	theme	Pse	984:986	arg1	labeling					998:1005	bacterial Pse metabolic labeling	974:1005	bacterial Pse metabolic labeling	974:1005	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	3	19	theme	nonulosonic	371:381	arg1	carbohydrates					388:400	nonulosonic acid carbohydrates	371:400	nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid	371:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	19	theme	nonulosonic	371:381	arg1	acid					329:332	Legionaminic acid	316:332	Legionaminic acid (Leg)	316:338	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	19	theme	nonulosonic	371:381	arg1	acid					356:359	pseudaminic acid	344:359	pseudaminic acid (Pse)	344:365	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	6	20	theme	aeruginosa	1089:1098	arg1	strains					1148:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains.					
30230814	3	21	with	carbohydrates	388:400	arg1	similarity					418:427	structural similarity	407:427	structural similarity to eukaryotic sialic acid	407:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	4	22	theme	immune	663:668	arg1	mechanism					677:685	immune escape mechanism	663:685	immune escape mechanism	663:685	They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism.					
30230814	3	23	theme	acid	383:386	arg1	carbohydrates					388:400	nonulosonic acid carbohydrates	371:400	nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid	371:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	23	theme	acid	383:386	arg1	acid					329:332	Legionaminic acid	316:332	Legionaminic acid (Leg)	316:338	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	23	theme	acid	383:386	arg1	acid					356:359	pseudaminic acid	344:359	pseudaminic acid (Pse)	344:365	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	4	24	theme	host	597:600	arg1	interaction					602:612	the host interaction	593:612	the host interaction	593:612	They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism.					
30230814	3	25	theme	glycans	563:569	arg1	components					525:534	components	525:534	components of cell surface-associated glycans	525:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	26	theme	numerous	479:486	arg1	bacteria					513:520	numerous pathogenic Gram-negative bacteria	479:520	numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans	479:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	0	27	theme	Glycans	50:56	arg1	Labeling					10:17	Metabolic Labeling	0:17	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces	0:78	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	2	28	theme	novel	247:251	arg1	targets					307:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	5	29	theme	chemical	945:952	arg1	Alt-4NAz					913:920	Alt-4NAz	913:920	Alt-4NAz	913:920	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	5	29	theme	chemical	945:952	arg1	reporter					954:961	an effective chemical reporter	932:961	an effective chemical reporter to realize bacterial Pse metabolic labeling	932:1005	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	0	30	theme	Acid-Containing	34:48	arg1	Glycans					50:56	Pseudaminic Acid-Containing Glycans	22:56	Pseudaminic Acid-Containing Glycans	22:56	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	3	31	theme	eukaryotic	432:441	arg1	acid					450:453	eukaryotic sialic acid	432:453	eukaryotic sialic acid	432:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	2	32	theme	antibacterial	178:190	arg1	drugs					192:196	new antibacterial drugs	174:196	new antibacterial drugs calls	174:202	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	0	33	theme	Pseudaminic	22:32	arg1	Glycans					50:56	Pseudaminic Acid-Containing Glycans	22:56	Pseudaminic Acid-Containing Glycans	22:56	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	4	34	theme	escape	670:675	arg1	mechanism					677:685	immune escape mechanism	663:685	immune escape mechanism	663:685	They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism.					
30230814	0	35	theme	Bacterial	61:69	arg1	Surfaces					71:78	Bacterial Surfaces	61:78	Bacterial Surfaces	61:78	Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces.					
30230814	5	36	theme	metabolic	988:996	arg1	labeling					998:1005	bacterial Pse metabolic labeling	974:1005	bacterial Pse metabolic labeling	974:1005	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	2	37	theme	new	174:176	arg1	drugs					192:196	new antibacterial drugs	174:196	new antibacterial drugs calls	174:202	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	5	38	theme	biological	713:722	arg1	significance					724:735	their biological significance	707:735	their biological significance	707:735	To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling.					
30230814	6	39	theme	reporter	1043:1050	arg1	effectiveness					1012:1024	The effectiveness	1008:1024	The effectiveness of this chemical reporter	1008:1050	The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains.					
30230814	3	40	theme	Legionaminic	316:327	arg1	carbohydrates					388:400	nonulosonic acid carbohydrates	371:400	nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid	371:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	40	theme	Legionaminic	316:327	arg1	acid					329:332	Legionaminic acid	316:332	Legionaminic acid (Leg)	316:338	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	40	theme	Legionaminic	316:327	arg1	acid					356:359	pseudaminic acid	344:359	pseudaminic acid (Pse)	344:365	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	40	theme	Legionaminic	316:327	arg1	Leg					335:337	Leg	335:337	Leg	335:337	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	41	theme	pathogenic	488:497	arg1	bacteria					513:520	numerous pathogenic Gram-negative bacteria	479:520	numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans	479:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	4	42	theme	defense	640:646	arg1	mechanism					648:656	antiphage defense mechanism	630:656	antiphage defense mechanism	630:656	They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism.					
30230814	2	43	theme	new	208:210	arg1	thinking					212:219	new thinking	208:219	new thinking	208:219	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	3	44	theme	Gram-negative	499:511	arg1	bacteria					513:520	numerous pathogenic Gram-negative bacteria	479:520	numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans	479:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	4	45	theme	antiphage	630:638	arg1	mechanism					648:656	antiphage defense mechanism	630:656	antiphage defense mechanism	630:656	They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism.					
30230814	3	46	theme	pseudaminic	344:354	arg1	carbohydrates					388:400	nonulosonic acid carbohydrates	371:400	nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid	371:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	46	theme	pseudaminic	344:354	arg1	Pse					362:364	Pse	362:364	Pse	362:364	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	46	theme	pseudaminic	344:354	arg1	acid					356:359	pseudaminic acid	344:359	pseudaminic acid (Pse)	344:365	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	3	46	theme	pseudaminic	344:354	arg1	acid					329:332	Legionaminic acid	316:332	Legionaminic acid (Leg)	316:338	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	2	47	theme	narrow-spectrum	254:268	arg1	targets					307:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	novel, narrow-spectrum, and pathogen-specific antibacterial targets	247:313	The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets.					
30230814	3	48	theme	cell	539:542	arg1	glycans					563:569	cell surface-associated glycans	539:569	cell surface-associated glycans	539:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	6	49	theme	baumannii	1138:1146	arg1	strains					1148:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains	1077:1154	The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains.					
30230814	3	50	theme	structural	407:416	arg1	similarity					418:427	structural similarity	407:427	structural similarity to eukaryotic sialic acid	407:453	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30230814	6	51	theme	chemical	1034:1041	arg1	reporter					1043:1050	this chemical reporter	1029:1050	this chemical reporter	1029:1050	The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains.					
30230814	3	52	theme	surface-associated	544:561	arg1	glycans					563:569	cell surface-associated glycans	539:569	cell surface-associated glycans	539:569	Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans.					
30977143	0	0	theme	acacia	80:85	arg1	properties					41:50	structural and functional properties	15:50	structural and functional properties of whey protein isolate-gum acacia	15:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	5	1	theme	high-performance	767:782	arg1	chromatography					799:812	high-performance size exclusion chromatography	767:812	high-performance size exclusion chromatography	767:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	1	theme	high-performance	767:782	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	6	2	theme	WPI-GA	906:911	arg1	conjugates					913:922	WPI-GA conjugates	906:922	WPI-GA conjugates incubated for 1, 3, 5, and 7 days	906:956	The degrees of glycation for WPI-GA conjugates incubated for 1, 3, 5, and 7 days were 28.14%, 44.98%, 49.50%, and 51.20%, respectively.					
30977143	9	3	theme	browning	1371:1378	arg1	degree					1361:1366	a high degree	1354:1366	a high degree of browning	1354:1378	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
30977143	0	4	theme	isolate-gum	68:78	arg1	acacia					80:85	whey protein isolate-gum acacia	55:85	whey protein isolate-gum acacia	55:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	0	5	from	effect	5:10	arg1	properties					41:50	structural and functional properties	15:50	structural and functional properties of whey protein isolate-gum acacia	15:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	7	6	theme	WPI	1101:1103	arg1	hydrophobicity					1056:1069	surface hydrophobicity	1048:1069	surface hydrophobicity	1048:1069	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	7	6	theme	WPI	1101:1103	arg1	intensity					1088:1096	fluorescence intensity	1075:1096	fluorescence intensity	1075:1096	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	1	7	theme	whey	170:173	arg1	WPI					192:194	WPI	192:194	WPI	192:194	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	1	7	theme	whey	170:173	arg1	isolate					183:189	whey protein isolate	170:189	whey protein isolate (WPI)	170:195	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	8	8	theme	heat-induced	1210:1221	arg1	insolubility					1223:1234	heat-induced insolubility	1210:1234	heat-induced insolubility	1210:1234	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties were all superior to the control WPI.					
30977143	11	9	theme	reaction	1594:1601	arg1	degree					1720:1725	the highest glycation degree	1698:1725	the highest glycation degree that could be reached	1698:1747	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	11	9	theme	reaction	1594:1601	arg1	time					1603:1606	the reaction time	1590:1606	the reaction time suitable for producing conjugates with superior functional properties	1590:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	8	10	theme	Functional	1131:1140	arg1	properties					1142:1151	Functional properties	1131:1151	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties	1131:1259	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties were all superior to the control WPI.					
30977143	7	11	theme	fluorescence	1075:1086	arg1	intensity					1088:1096	fluorescence intensity	1075:1096	fluorescence intensity	1075:1096	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	10	12	with	conjugation	1493:1503	arg1	GA					1517:1518	GA	1517:1518	GA	1517:1518	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	1	13	dep	BACKGROUND	130:139	arg1	properties					156:165	The functional properties	141:165	The functional properties of whey protein isolate (WPI)	141:195	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	1	13	dep	BACKGROUND	130:139	arg1	sensitive					201:209	sensitive	201:209	sensitive	201:209	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	4	14	theme	Maillard	561:568	arg1	reaction					570:577	the dry-heating Maillard reaction	545:577	the dry-heating Maillard reaction	545:577	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	3	15	theme	proteins	353:360	arg1	conjugation					338:348	The conjugation	334:348	The conjugation of proteins with polysaccharides via the Maillard reaction	334:407	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	3	15	theme	proteins	353:360	arg1	method					425:430	an efficient method	412:430	an efficient method to improve the functionality of proteins	412:471	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	10	16	dep	CONCLUSION	1455:1464	arg1	indicated					1478:1486	indicated	1478:1486	indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties	1478:1578	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	10	17	theme	functional	1558:1567	arg1	properties					1569:1578	its functional properties	1554:1578	its functional properties	1554:1578	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	4	18	theme	dry-heating	549:559	arg1	reaction					570:577	the dry-heating Maillard reaction	545:577	the dry-heating Maillard reaction	545:577	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	0	19	theme	Maillard	111:118	arg1	reaction					120:127	Maillard reaction	111:127	Maillard reaction	111:127	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	5	20	theme	conjugates	865:874	arg1	formation					828:836	the formation	824:836	the formation of higher molecular weight conjugates	824:874	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	4	21	theme	physicochemical	633:647	arg1	properties					664:673	the physicochemical and functional properties	629:673	the physicochemical and functional properties of WPI-GA conjugates	629:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	1	22	theme	protein	175:181	arg1	WPI					192:194	WPI	192:194	WPI	192:194	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	1	22	theme	protein	175:181	arg1	isolate					183:189	whey protein isolate	170:189	whey protein isolate (WPI)	170:195	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	5	23	theme	gel	743:745	arg1	electrophoresis					747:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	23	theme	gel	743:745	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	11	24	theme	highest	1702:1708	arg1	degree					1720:1725	the highest glycation degree	1698:1725	the highest glycation degree that could be reached	1698:1747	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	11	24	theme	highest	1702:1708	arg1	time					1603:1606	the reaction time	1590:1606	the reaction time suitable for producing conjugates with superior functional properties	1590:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	5	25	theme	exclusion	789:797	arg1	chromatography					799:812	high-performance size exclusion chromatography	767:812	high-performance size exclusion chromatography	767:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	25	theme	exclusion	789:797	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	0	26	dep	conjugates	87:96	arg1	prepared					98:105	prepared	98:105	conjugates prepared via Maillard reaction	87:127	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	7	27	dep	hydrophobicity	1056:1069	arg1	the					1044:1046	the	1044:1046	the	1044:1046	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	1	28	theme	isolate	183:189	arg1	properties					156:165	The functional properties	141:165	The functional properties of whey protein isolate (WPI)	141:195	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	1	28	theme	isolate	183:189	arg1	sensitive					201:209	sensitive	201:209	sensitive	201:209	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	8	29	theme	conjugates	1160:1169	arg1	properties					1142:1151	Functional properties	1131:1151	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties	1131:1259	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties were all superior to the control WPI.					
30977143	5	30	theme	size	784:787	arg1	chromatography					799:812	high-performance size exclusion chromatography	767:812	high-performance size exclusion chromatography	767:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	30	theme	size	784:787	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	3	31	theme	efficient	415:423	arg1	conjugation					338:348	The conjugation	334:348	The conjugation of proteins with polysaccharides via the Maillard reaction	334:407	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	3	31	theme	efficient	415:423	arg1	method					425:430	an efficient method	412:430	an efficient method to improve the functionality of proteins	412:471	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	0	32	theme	Time	0:3	arg1	effect					5:10	Time effect	0:10	Time effect on structural and functional properties of whey protein isolate-gum acacia	0:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	7	33	theme	surface	1048:1054	arg1	hydrophobicity					1056:1069	surface hydrophobicity	1048:1069	surface hydrophobicity	1048:1069	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	9	34	theme	longer	1324:1329	arg1	time					1319:1322	a reaction time	1308:1322	a reaction time longer than a day	1308:1340	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
30977143	2	35	theme	food	292:295	arg1	systems					297:303	various food systems	284:303	various food systems	284:303	This prevents its application in various food systems and processing technologies.					
30977143	11	36	with	conjugates	1631:1640	arg1	properties					1667:1676	superior functional properties	1647:1676	superior functional properties	1647:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	11	37	theme	functional	1656:1665	arg1	properties					1667:1676	superior functional properties	1647:1676	superior functional properties	1647:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	0	38	theme	structural	15:24	arg1	properties					41:50	structural and functional properties	15:50	structural and functional properties of whey protein isolate-gum acacia	15:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	11	39	theme	suitable	1608:1615	arg1	degree					1720:1725	the highest glycation degree	1698:1725	the highest glycation degree that could be reached	1698:1747	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	11	39	theme	suitable	1608:1615	arg1	time					1603:1606	the reaction time	1590:1606	the reaction time suitable for producing conjugates with superior functional properties	1590:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	2	40	theme	various	284:290	arg1	systems					297:303	various food systems	284:303	various food systems	284:303	This prevents its application in various food systems and processing technologies.					
30977143	5	41	theme	weight	858:863	arg1	conjugates					865:874	higher molecular weight conjugates	841:874	higher molecular weight conjugates	841:874	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	4	42	with	acacia	520:525	arg1	WPI					537:539	WPI	537:539	WPI	537:539	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	9	43	theme	reaction	1310:1317	arg1	time					1319:1322	a reaction time	1308:1322	a reaction time longer than a day	1308:1340	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
30977143	11	44	theme	superior	1647:1654	arg1	properties					1667:1676	superior functional properties	1647:1676	superior functional properties	1647:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	10	45	theme	WPI	1508:1510	arg1	conjugation					1493:1503	conjugation	1493:1503	conjugation of WPI with GA	1493:1518	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	10	45	theme	WPI	1508:1510	arg1	way					1539:1541	a promising way	1527:1541	a promising way to enhance its functional properties	1527:1578	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	4	46	from	effect	602:607	arg1	properties					664:673	the physicochemical and functional properties	629:673	the physicochemical and functional properties of WPI-GA conjugates	629:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	3	47	theme	proteins	464:471	arg1	functionality					447:459	the functionality	443:459	the functionality of proteins	443:471	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	0	48	theme	functional	30:39	arg1	properties					41:50	structural and functional properties	15:50	structural and functional properties of whey protein isolate-gum acacia	15:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	6	49	theme	glycation	892:900	arg1	%					968:968	28.14%	963:968	28.14%	963:968	The degrees of glycation for WPI-GA conjugates incubated for 1, 3, 5, and 7 days were 28.14%, 44.98%, 49.50%, and 51.20%, respectively.					
30977143	6	49	theme	glycation	892:900	arg1	degrees					881:887	The degrees	877:887	The degrees of glycation for WPI-GA conjugates incubated for 1, 3, 5, and 7 days	877:956	The degrees of glycation for WPI-GA conjugates incubated for 1, 3, 5, and 7 days were 28.14%, 44.98%, 49.50%, and 51.20%, respectively.					
30977143	4	50	theme	reaction	612:619	arg1	time					621:624	reaction time	612:624	reaction time	612:624	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	4	51	theme	functional	653:662	arg1	properties					664:673	the physicochemical and functional properties	629:673	the physicochemical and functional properties of WPI-GA conjugates	629:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	4	52	theme	gum	516:518	arg1	acacia					520:525	conjugate gum acacia	506:525	conjugate gum acacia (GA) with WPI	506:539	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	4	52	theme	gum	516:518	arg1	GA					528:529	GA	528:529	GA	528:529	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	7	53	theme	glycation	1017:1025	arg1	reaction					1027:1034	The glycation reaction	1013:1034	The glycation reaction	1013:1034	The glycation reaction reduced the surface hydrophobicity and fluorescence intensity of WPI significantly (P < 0.05).					
30977143	3	54	with	conjugation	338:348	arg1	polysaccharides					367:381	polysaccharides	367:381	polysaccharides via the Maillard reaction	367:407	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	12	55	theme	Chemical	1768:1775	arg1	Industry					1777:1784	Chemical Industry	1768:1784	Chemical Industry	1768:1784	© 2019 Society of Chemical Industry.					
30977143	5	56	theme	higher	841:846	arg1	conjugates					865:874	higher molecular weight conjugates	841:874	higher molecular weight conjugates	841:874	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	3	57	theme	Maillard	391:398	arg1	reaction					400:407	the Maillard reaction	387:407	the Maillard reaction	387:407	The conjugation of proteins with polysaccharides via the Maillard reaction is an efficient method to improve the functionality of proteins.					
30977143	4	58	theme	conjugate	506:514	arg1	acacia					520:525	conjugate gum acacia	506:525	conjugate gum acacia (GA) with WPI	506:539	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	4	58	theme	conjugate	506:514	arg1	GA					528:529	GA	528:529	GA	528:529	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	5	59	dep	RESULTS	697:703	arg1	chromatography					799:812	high-performance size exclusion chromatography	767:812	high-performance size exclusion chromatography	767:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	59	dep	RESULTS	697:703	arg1	electrophoresis					747:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	59	dep	RESULTS	697:703	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	9	60	dep	decreased	1384:1392	arg1	P < 0.05					1444:1451	P < 0.05	1444:1451	P < 0.05	1444:1451	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
30977143	4	61	theme	conjugates	685:694	arg1	properties					664:673	the physicochemical and functional properties	629:673	the physicochemical and functional properties of WPI-GA conjugates	629:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	9	62	theme	conjugate	1419:1427	arg1	functionality					1398:1410	the functionality	1394:1410	the functionality of the conjugate	1394:1427	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
30977143	4	63	theme	WPI-GA	678:683	arg1	conjugates					685:694	WPI-GA conjugates	678:694	WPI-GA conjugates	678:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	2	64	theme	processing	309:318	arg1	technologies					320:331	processing technologies	309:331	processing technologies	309:331	This prevents its application in various food systems and processing technologies.					
30977143	5	65	theme	sulfate-polyacrylamide	720:741	arg1	electrophoresis					747:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	705:761	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	5	65	theme	sulfate-polyacrylamide	720:741	arg1	RESULTS					697:703	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography	697:812	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	2	66	from	application	269:279	arg1	technologies					320:331	processing technologies	309:331	processing technologies	309:331	This prevents its application in various food systems and processing technologies.					
30977143	2	66	from	application	269:279	arg1	systems					297:303	various food systems	284:303	various food systems	284:303	This prevents its application in various food systems and processing technologies.					
30977143	0	67	theme	protein	60:66	arg1	acacia					80:85	whey protein isolate-gum acacia	55:85	whey protein isolate-gum acacia	55:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	11	68	theme	glycation	1710:1718	arg1	degree					1720:1725	the highest glycation degree	1698:1725	the highest glycation degree that could be reached	1698:1747	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	11	68	theme	glycation	1710:1718	arg1	time					1603:1606	the reaction time	1590:1606	the reaction time suitable for producing conjugates with superior functional properties	1590:1676	However, the reaction time suitable for producing conjugates with superior functional properties was not necessarily the highest glycation degree that could be reached.					
30977143	8	69	theme	emulsion	1241:1248	arg1	properties					1250:1259	emulsion properties	1241:1259	emulsion properties	1241:1259	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties were all superior to the control WPI.					
30977143	1	70	theme	functional	145:154	arg1	properties					156:165	The functional properties	141:165	The functional properties of whey protein isolate (WPI)	141:195	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	1	70	theme	functional	145:154	arg1	sensitive					201:209	sensitive	201:209	sensitive	201:209	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	4	71	theme	time	621:624	arg1	effect					602:607	the effect	598:607	the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates	598:694	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	1	72	theme	ionic	218:222	arg1	strength					224:231	ionic strength	218:231	ionic strength	218:231	BACKGROUND The functional properties of whey protein isolate (WPI) are sensitive to pH, ionic strength, and temperature.					
30977143	0	73	theme	whey	55:58	arg1	acacia					80:85	whey protein isolate-gum acacia	55:85	whey protein isolate-gum acacia	55:85	Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction.					
30977143	4	74	theme	work	494:497	arg1	purpose					478:484	The purpose	474:484	The purpose of this work	474:497	The purpose of this work was to conjugate gum acacia (GA) with WPI via the dry-heating Maillard reaction and to investigate the effect of reaction time on the physicochemical and functional properties of WPI-GA conjugates.					
30977143	5	75	theme	molecular	848:856	arg1	conjugates					865:874	higher molecular weight conjugates	841:874	higher molecular weight conjugates	841:874	RESULTS Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance size exclusion chromatography confirmed the formation of higher molecular weight conjugates.					
30977143	10	76	theme	promising	1529:1537	arg1	conjugation					1493:1503	conjugation	1493:1503	conjugation of WPI with GA	1493:1518	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	10	76	theme	promising	1529:1537	arg1	way					1539:1541	a promising way	1527:1541	a promising way to enhance its functional properties	1527:1578	CONCLUSION The results indicated that conjugation of WPI with GA can be a promising way to enhance its functional properties.					
30977143	8	77	theme	control	1286:1292	arg1	WPI					1294:1296	the control WPI	1282:1296	the control WPI	1282:1296	Functional properties of the conjugates, such as solubility, stability against heat-induced insolubility, and emulsion properties were all superior to the control WPI.					
30977143	9	78	theme	high	1356:1359	arg1	degree					1361:1366	a high degree	1354:1366	a high degree of browning	1354:1378	However, a reaction time longer than a day resulted in a high degree of browning and decreased the functionality of the conjugate significantly (P < 0.05).					
29698574	11	0	theme	proximal	2016:2023	arg1	fluids					2038:2043	proximal breast tumor fluids	2016:2043	proximal breast tumor fluids	2016:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	8	1	theme	poor	1616:1619	arg1	prognosis					1621:1629	poor prognosis	1616:1629	poor prognosis	1616:1629	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	3	2	theme	normal	699:704	arg1	fluids					719:724	paired normal interstitial fluids	692:724	paired normal interstitial fluids (NIF, n = 54)	692:738	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	9	3	theme	GP1	1642:1644	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	6	4	theme	N-glycans	1193:1201	arg1	level					1164:1168	An increased level	1151:1168	An increased level of simple high mannose N-glycans in TIF	1151:1208	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	9	5	theme	GP8	1647:1649	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	6	6	theme	high	1180:1183	arg1	N-glycans					1193:1201	simple high mannose N-glycans	1173:1201	simple high mannose N-glycans	1173:1201	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	6	7	theme	lymphocytes	1270:1280	arg1	presence					1239:1246	the presence	1235:1246	the presence of tumor infiltrating lymphocytes within tumor	1235:1293	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	9	8	theme	GP9	1652:1654	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	7	9	theme	infiltrating	1403:1414	arg1	lymphocytes					1416:1426	infiltrating lymphocytes	1403:1426	infiltrating lymphocytes	1403:1426	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	6	10	theme	tumor	1251:1255	arg1	lymphocytes					1270:1280	tumor infiltrating lymphocytes	1251:1280	tumor infiltrating lymphocytes	1251:1280	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	9	11	theme	GP14	1657:1660	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	6	12	theme	increased	1154:1162	arg1	level					1164:1168	An increased level	1151:1168	An increased level of simple high mannose N-glycans in TIF	1151:1208	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	11	13	theme	tumor	2032:2036	arg1	fluids					2038:2043	proximal breast tumor fluids	2016:2043	proximal breast tumor fluids	2016:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	0	14	theme	tumor	113:117	arg1	biology					119:125	tumor biology	113:125	tumor biology	113:125	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	1	15	used	used	371:374	arg2	biomarkers					434:443	predictive or prognostic biomarkers	409:443	predictive or prognostic biomarkers	409:443	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	1	15	used	used	371:374	arg2	patterns					355:362	N-glycan patterns	346:362	N-glycan patterns	346:362	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	4	16	theme	N-linked	933:940	arg1	oligosaccharides					942:957	N-linked oligosaccharides	933:957	N-linked oligosaccharides	933:957	The segregation of N-linked oligosaccharides revealed 33 compositions, which exhibited differential abundances between TIF and NIF.					
29698574	1	17	theme	predictive	409:418	arg1	biomarkers					434:443	predictive or prognostic biomarkers	409:443	predictive or prognostic biomarkers	409:443	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	1	17	theme	predictive	409:418	arg1	patterns					355:362	N-glycan patterns	346:362	N-glycan patterns	346:362	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	11	18	theme	promising	2050:2058	arg1	profiling					1988:1996	profiling	1988:1996	profiling of N-glycans from proximal breast tumor fluids	1988:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	11	18	theme	promising	2050:2058	arg1	strategy					2060:2067	a promising strategy	2048:2067	a promising strategy for determining tumor-derived glyco-signature(s) in the blood	2048:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	1	19	theme	breast	212:217	arg1	malignancies					219:230	breast malignancies	212:230	breast malignancies	212:230	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	9	20	theme	GP28	1669:1672	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	11	21	from	s	2115:2115	arg1	blood					2125:2129	the blood	2121:2129	the blood	2121:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	1	22	theme	prognostic	423:432	arg1	biomarkers					434:443	predictive or prognostic biomarkers	409:443	predictive or prognostic biomarkers	409:443	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	1	22	theme	prognostic	423:432	arg1	patterns					355:362	N-glycan patterns	346:362	N-glycan patterns	346:362	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	11	23	theme	N-glycans	2001:2009	arg1	profiling					1988:1996	profiling	1988:1996	profiling of N-glycans from proximal breast tumor fluids	1988:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	11	23	theme	N-glycans	2001:2009	arg1	strategy					2060:2067	a promising strategy	2048:2067	a promising strategy for determining tumor-derived glyco-signature(s) in the blood	2048:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	3	24	theme	N-glycan	855:862	arg1	patterns					864:871	tumor-related N-glycan patterns	841:871	tumor-related N-glycan patterns in blood of patients with breast cancer	841:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	9	25	theme	GP37	1675:1678	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	3	26	theme	interstitial	657:668	arg1	fluids					670:675	tumor interstitial fluids	651:675	tumor interstitial fluids (TIF, n = 85)	651:689	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	27	dep	profiled	579:586	arg1	followed					767:774	followed	767:774	followed	767:774	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	9	28	theme	GP38	1681:1684	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	8	29	theme	TIF	1502:1504	arg1	abundance					1506:1514	increased TIF abundance	1492:1514	increased TIF abundance of GP24	1492:1522	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	7	30	theme	complex	1336:1342	arg1	N-glycans					1344:1352	highly complex N-glycans	1329:1352	highly complex N-glycans	1329:1352	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	3	31	theme	independent	779:789	arg1	evaluation					791:800	independent evaluation	779:800	independent evaluation	779:800	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	1	32	theme	N-glycan	346:353	arg1	biomarkers					434:443	predictive or prognostic biomarkers	409:443	predictive or prognostic biomarkers	409:443	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	1	32	theme	N-glycan	346:353	arg1	patterns					355:362	N-glycan patterns	346:362	N-glycan patterns	346:362	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	4	33	theme	differential	1001:1012	arg1	abundances					1014:1023	differential abundances	1001:1023	differential abundances between TIF and NIF	1001:1043	The segregation of N-linked oligosaccharides revealed 33 compositions, which exhibited differential abundances between TIF and NIF.					
29698574	1	34	theme	corresponding	295:307	arg1	microenvironment					309:324	the tumor and corresponding microenvironment	281:324	microenvironment	309:324	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	0	35	theme	breast	72:77	arg1	patients					86:93	breast cancer patients	72:93	breast cancer patients	72:93	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	1	36	theme	N-glycan	160:167	arg1	structures					169:178	Particular N-glycan structures	149:178	Particular N-glycan structures	149:178	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	10	37	theme	Cross-validation	1765:1780	arg1	analysis					1782:1789	Cross-validation analysis	1765:1789	Cross-validation analysis using an independent serum dataset	1765:1824	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	12	38	theme	N-glycans	2132:2140	arg1	structures					2142:2151	N-glycans structures	2132:2151	N-glycans structures validated in our study	2132:2174	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	12	38	theme	N-glycans	2132:2140	arg1	biomarkers					2195:2204	novel biomarkers	2189:2204	novel biomarkers	2189:2204	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	0	39	with	outcome	140:146	arg1	biology					119:125	tumor biology	113:125	tumor biology	113:125	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	0	40	theme	patients	86:93	arg1	serum					63:67	serum	63:67	serum	63:67	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	0	40	theme	patients	86:93	arg1	fluid					53:57	tumor interstitial fluid	34:57	tumor interstitial fluid	34:57	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	0	41	dep	association	96:106	arg1	signatures					9:18	N-glycan signatures	0:18	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients	0:93	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	1	42	theme	cancer	380:385	arg1	stratification					387:400	cancer stratification	380:400	cancer stratification	380:400	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	2	43	theme	clinical	532:539	arg1	relevance					541:549	corresponding clinical relevance	518:549	corresponding clinical relevance	518:549	However, the association between N-glycans secreted by breast tumor and corresponding clinical relevance remain to be elucidated.					
29698574	0	44	theme	N-glycan	0:7	arg1	signatures					9:18	N-glycan signatures	0:18	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients	0:93	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	1	45	theme	tumor	285:289	arg1	microenvironment					309:324	the tumor and corresponding microenvironment	281:324	microenvironment	309:324	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	9	46	theme	paired	1743:1748	arg1	samples					1756:1762	paired serum samples	1743:1762	paired serum samples	1743:1762	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	3	47	dep	fluids	719:724	arg1	n = 54					732:737	n = 54	732:737	n = 54	732:737	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	47	dep	fluids	719:724	arg1	NIF					727:729	NIF	727:729	NIF	727:729	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	2	48	theme	breast	501:506	arg1	tumor					508:512	breast tumor	501:512	breast tumor	501:512	However, the association between N-glycans secreted by breast tumor and corresponding clinical relevance remain to be elucidated.					
29698574	8	49	theme	GP10	1567:1570	arg1	levels					1557:1562	low levels	1553:1562	low levels of GP10, GP23, GP38, and coreF	1553:1593	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	10	50	theme	independent	1800:1810	arg1	dataset					1818:1824	an independent serum dataset	1797:1824	an independent serum dataset	1797:1824	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	6	51	from	level	1164:1168	arg1	TIF					1206:1208	TIF	1206:1208	TIF	1206:1208	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	0	52	theme	tumor	34:38	arg1	fluid					53:57	tumor interstitial fluid	34:57	tumor interstitial fluid	34:57	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	11	53	theme	tumor-derived	2085:2097	arg1	s					2115:2115	tumor-derived glyco-signature(s)	2085:2116	tumor-derived glyco-signature(s) in the blood	2085:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	8	54	theme	GP23	1573:1576	arg1	levels					1557:1562	low levels	1553:1562	low levels of GP10, GP23, GP38, and coreF	1553:1593	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	8	55	theme	GP38	1579:1582	arg1	levels					1557:1562	low levels	1553:1562	low levels of GP10, GP23, GP38, and coreF	1553:1593	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	3	56	theme	paired	692:697	arg1	fluids					719:724	paired normal interstitial fluids	692:724	paired normal interstitial fluids (NIF, n = 54)	692:738	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	57	dep	fluids	670:675	arg1	TIF					678:680	TIF	678:680	TIF	678:680	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	57	dep	fluids	670:675	arg1	n = 85					683:688	n = 85	683:688	n = 85	683:688	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	4	58	link	N-linked	933:940	arg1	oligosaccharides					942:957	N-linked oligosaccharides	933:957	N-linked oligosaccharides	933:957	The segregation of N-linked oligosaccharides revealed 33 compositions, which exhibited differential abundances between TIF and NIF.					
29698574	8	59	theme	Survival	1442:1449	arg1	analysis					1451:1458	Survival analysis	1442:1458	Survival analysis	1442:1458	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	1	60	theme	regulatory	256:265	arg1	events					267:272	various regulatory events	248:272	various regulatory events within the tumor and corresponding microenvironment	248:324	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	11	61	theme	breast	2025:2030	arg1	fluids					2038:2043	proximal breast tumor fluids	2016:2043	proximal breast tumor fluids	2016:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	12	62	theme	patients	2265:2272	arg1	stratification					2247:2260	the diagnostic and prognostic stratification	2217:2260	the diagnostic and prognostic stratification of patients with breast cancer	2217:2291	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	3	63	theme	interstitial	706:717	arg1	fluids					719:724	paired normal interstitial fluids	692:724	paired normal interstitial fluids (NIF, n = 54)	692:738	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	6	64	theme	mannose	1185:1191	arg1	N-glycans					1193:1201	simple high mannose N-glycans	1173:1201	simple high mannose N-glycans	1173:1201	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	12	65	theme	breast	2279:2284	arg1	cancer					2286:2291	breast cancer	2279:2291	breast cancer	2279:2291	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	6	66	theme	simple	1173:1178	arg1	N-glycans					1193:1201	simple high mannose N-glycans	1173:1201	simple high mannose N-glycans	1173:1201	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	6	67	theme	infiltrating	1257:1268	arg1	lymphocytes					1270:1280	tumor infiltrating lymphocytes	1251:1280	tumor infiltrating lymphocytes	1251:1280	An increased level of simple high mannose N-glycans in TIF strongly correlated with the presence of tumor infiltrating lymphocytes within tumor.					
29698574	0	68	with	association	96:106	arg1	biology					119:125	tumor biology	113:125	tumor biology	113:125	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	3	69	theme	HILIC	601:605	arg1	UPLC					607:610	HILIC UPLC	601:610	HILIC UPLC	601:610	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	7	70	theme	lymphocytes	1416:1426	arg1	presence					1391:1398	the presence	1387:1398	the presence of infiltrating lymphocytes within tumor	1387:1439	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	8	71	contain	had	1524:1526	arg1	patients					1472:1479	patients	1472:1479	patients exhibiting increased TIF abundance of GP24	1472:1522	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	8	71	contain	had	1524:1526	arg2	outcomes					1535:1542	better outcomes	1528:1542	better outcomes	1528:1542	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	GP23					1934:1937	GP23	1934:1937	GP23	1934:1937	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	GP8					1918:1920	GP8	1918:1920	GP8	1918:1920	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	GP9					1923:1925	GP9	1923:1925	GP9	1923:1925	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	coreF					1944:1948	coreF	1944:1948	coreF	1944:1948	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	groups					1910:1915	five of nine N-glycan groups	1888:1915	five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF	1888:1948	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	72	theme	N-glycan	1901:1908	arg1	GP14					1928:1931	GP14	1928:1931	GP14	1928:1931	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	12	73	theme	prognostic	2236:2245	arg1	stratification					2247:2260	the diagnostic and prognostic stratification	2217:2260	the diagnostic and prognostic stratification of patients with breast cancer	2217:2291	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	3	74	from	patterns	864:871	arg1	blood					876:880	blood	876:880	blood of patients with breast cancer	876:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	75	theme	serum	744:748	arg1	samples					750:756	serum samples	744:756	serum samples (n = 28)	744:765	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	3	75	theme	serum	744:748	arg1	n = 28					759:764	n = 28	759:764	n = 28	759:764	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	0	76	theme	clinical	131:138	arg1	outcome					140:146	clinical outcome	131:146	clinical outcome	131:146	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	3	77	theme	discovery	621:629	arg1	dataset					631:637	a discovery dataset	619:637	a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28)	619:765	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	11	78	from	fluids	2038:2043	arg1	profiling					1988:1996	profiling	1988:1996	profiling of N-glycans from proximal breast tumor fluids	1988:2043	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	11	78	from	fluids	2038:2043	arg1	strategy					2060:2067	a promising strategy	2048:2067	a promising strategy for determining tumor-derived glyco-signature(s) in the blood	2048:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	12	79	theme	novel	2189:2193	arg1	structures					2142:2151	N-glycans structures	2132:2151	N-glycans structures validated in our study	2132:2174	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	12	79	theme	novel	2189:2193	arg1	biomarkers					2195:2204	novel biomarkers	2189:2204	novel biomarkers	2189:2204	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	8	80	theme	increased	1492:1500	arg1	abundance					1506:1514	increased TIF abundance	1492:1514	increased TIF abundance of GP24	1492:1522	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	3	81	theme	tumor	651:655	arg1	fluids					670:675	tumor interstitial fluids	651:675	tumor interstitial fluids (TIF, n = 85)	651:689	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	12	82	theme	diagnostic	2221:2230	arg1	stratification					2247:2260	the diagnostic and prognostic stratification	2217:2260	the diagnostic and prognostic stratification of patients with breast cancer	2217:2291	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	3	83	theme	tumor-related	841:853	arg1	patterns					864:871	tumor-related N-glycan patterns	841:871	tumor-related N-glycan patterns in blood of patients with breast cancer	841:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	2	84	theme	corresponding	518:530	arg1	relevance					541:549	corresponding clinical relevance	518:549	corresponding clinical relevance	518:549	However, the association between N-glycans secreted by breast tumor and corresponding clinical relevance remain to be elucidated.					
29698574	8	85	theme	GP24	1519:1522	arg1	abundance					1506:1514	increased TIF abundance	1492:1514	increased TIF abundance of GP24	1492:1522	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	7	86	theme	N-glycans	1344:1352	arg1	level					1320:1324	a low level	1314:1324	a low level of highly complex N-glycans in TIF	1314:1359	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	0	87	theme	cancer	79:84	arg1	patients					86:93	breast cancer patients	72:93	breast cancer patients	72:93	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	5	88	theme	tumor	1132:1136	arg1	suppression					1138:1148	tumor suppression	1132:1148	tumor suppression	1132:1148	TIFs were depleted of bisecting N-glycans, which are known to play essential roles in tumor suppression.					
29698574	1	89	theme	Particular	149:158	arg1	structures					169:178	Particular N-glycan structures	149:178	Particular N-glycan structures	149:178	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
29698574	3	90	theme	patients	885:892	arg1	blood					876:880	blood	876:880	blood of patients with breast cancer	876:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	9	91	theme	coreF	1691:1695	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	8	92	theme	better	1528:1533	arg1	outcomes					1535:1542	better outcomes	1528:1542	better outcomes	1528:1542	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	3	93	with	patients	885:892	arg1	cancer					906:911	breast cancer	899:911	breast cancer	899:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	4	94	theme	oligosaccharides	942:957	arg1	segregation					918:928	The segregation	914:928	The segregation of N-linked oligosaccharides	914:957	The segregation of N-linked oligosaccharides revealed 33 compositions, which exhibited differential abundances between TIF and NIF.					
29698574	3	95	theme	ultimate	812:819	arg1	goal					821:824	the ultimate goal	808:824	the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer	808:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	8	96	theme	low	1553:1555	arg1	levels					1557:1562	low levels	1553:1562	low levels of GP10, GP23, GP38, and coreF	1553:1593	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	3	97	theme	breast	899:904	arg1	cancer					906:911	breast cancer	899:911	breast cancer	899:911	We profiled N-glycans by HILIC UPLC across a discovery dataset composed of tumor interstitial fluids (TIF, n = 85), paired normal interstitial fluids (NIF, n = 54) and serum samples (n = 28) followed by independent evaluation, with the ultimate goal of identifying tumor-related N-glycan patterns in blood of patients with breast cancer.					
29698574	7	98	theme	low	1316:1318	arg1	level					1320:1324	a low level	1314:1324	a low level of highly complex N-glycans in TIF	1314:1359	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	9	99	theme	GP23	1663:1666	arg1	Levels					1632:1637	Levels	1632:1637	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF	1632:1695	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	11	100	link	tumor-derived	2085:2097	arg1	s					2115:2115	tumor-derived glyco-signature(s)	2085:2116	tumor-derived glyco-signature(s) in the blood	2085:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	9	101	theme	serum	1750:1754	arg1	samples					1756:1762	paired serum samples	1743:1762	paired serum samples	1743:1762	Levels of GP1, GP8, GP9, GP14, GP23, GP28, GP37, GP38, and coreF were significantly correlated between TIF and paired serum samples.					
29698574	10	102	theme	serum	1812:1816	arg1	dataset					1818:1824	an independent serum dataset	1797:1824	an independent serum dataset	1797:1824	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	0	103	theme	interstitial	40:51	arg1	fluid					53:57	tumor interstitial fluid	34:57	tumor interstitial fluid	34:57	N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome.					
29698574	10	104	dep	groups	1910:1915	arg1	GP23					1934:1937	GP23	1934:1937	GP23	1934:1937	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	104	dep	groups	1910:1915	arg1	GP8					1918:1920	GP8	1918:1920	GP8	1918:1920	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	104	dep	groups	1910:1915	arg1	GP9					1923:1925	GP9	1923:1925	GP9	1923:1925	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	104	dep	groups	1910:1915	arg1	coreF					1944:1948	coreF	1944:1948	coreF	1944:1948	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	104	dep	groups	1910:1915	arg1	groups					1910:1915	five of nine N-glycan groups	1888:1915	five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF	1888:1948	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	10	104	dep	groups	1910:1915	arg1	GP14					1928:1931	GP14	1928:1931	GP14	1928:1931	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	7	105	theme	same	1303:1306	arg1	time					1308:1311	the same time	1299:1311	the same time	1299:1311	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	5	106	theme	essential	1113:1121	arg1	roles					1123:1127	essential roles	1113:1127	essential roles	1113:1127	TIFs were depleted of bisecting N-glycans, which are known to play essential roles in tumor suppression.					
29698574	11	107	theme	glyco-signature	2099:2113	arg1	s					2115:2115	tumor-derived glyco-signature(s)	2085:2116	tumor-derived glyco-signature(s) in the blood	2085:2129	Collectively, our results imply that profiling of N-glycans from proximal breast tumor fluids is a promising strategy for determining tumor-derived glyco-signature(s) in the blood.					
29698574	10	108	theme	observed	1840:1847	arg1	correlation					1849:1859	the observed correlation	1836:1859	the observed correlation between TIF and serum	1836:1881	Cross-validation analysis using an independent serum dataset supported the observed correlation between TIF and serum, for five of nine N-glycan groups: GP8, GP9, GP14, GP23, and coreF.					
29698574	8	109	theme	coreF	1589:1593	arg1	levels					1557:1562	low levels	1553:1562	low levels of GP10, GP23, GP38, and coreF	1553:1593	Survival analysis showed that patients exhibiting increased TIF abundance of GP24 had better outcomes, whereas low levels of GP10, GP23, GP38, and coreF were associated with poor prognosis.					
29698574	12	110	with	patients	2265:2272	arg1	cancer					2286:2291	breast cancer	2279:2291	breast cancer	2279:2291	N-glycans structures validated in our study may serve as novel biomarkers to improve the diagnostic and prognostic stratification of patients with breast cancer.					
29698574	7	111	from	level	1320:1324	arg1	TIF					1357:1359	TIF	1357:1359	TIF	1357:1359	At the same time, a low level of highly complex N-glycans in TIF inversely correlated with the presence of infiltrating lymphocytes within tumor.					
29698574	1	112	theme	various	248:254	arg1	events					267:272	various regulatory events	248:272	various regulatory events within the tumor and corresponding microenvironment	248:324	Particular N-glycan structures are known to be associated with breast malignancies by coordinating various regulatory events within the tumor and corresponding microenvironment, thus implying that N-glycan patterns may be used for cancer stratification and as predictive or prognostic biomarkers.					
31104946	0	0	theme	Protective	84:93	arg1	Epitope					101:107	a Subdominant but Broadly Protective Viral Epitope	58:107	a Subdominant but Broadly Protective Viral Epitope	58:107	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31104946	4	1	theme	such	520:523	arg1	modifications					525:537	such modifications	520:537	such modifications	520:537	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	8	2	theme	next-generation	1109:1123	arg1	vaccines					1131:1138	next-generation viral vaccines	1109:1138	next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s)	1109:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	3	3	dep	Understanding	339:351	arg1	affects					371:377	affects	371:377	affects immunodominance on complex protein antigens	371:421	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	6	4	theme	antibody	863:870	arg1	family					877:882	one antibody gene family	859:882	one antibody gene family	859:882	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	5	5	theme	antibody	749:756	arg1	repertoire					758:767	the initially diverse antibody repertoire	727:767	the initially diverse antibody repertoire	727:767	We found that glycan addition changed the initially diverse antibody repertoire into an epitope-focused, genetically restricted response.					
31104946	7	6	theme	influenza	1030:1038	arg1	mice					1057:1060	influenza virus-challenged mice	1030:1060	influenza virus-challenged mice	1030:1060	Passive transfer of this antibody conferred Fc-dependent protection to influenza virus-challenged mice.					
31104946	3	7	theme	complex	398:404	arg1	antigens					414:421	complex protein antigens	398:421	complex protein antigens	398:421	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	6	8	theme	head	943:946	arg1	interface					948:956	the head interface	939:956	the head interface	939:956	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	8	9	theme	viral	1125:1129	arg1	vaccines					1131:1138	next-generation viral vaccines	1109:1138	next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s)	1109:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	7	10	theme	Fc-dependent	1003:1014	arg1	protection					1016:1025	Fc-dependent protection	1003:1025	Fc-dependent protection	1003:1025	Passive transfer of this antibody conferred Fc-dependent protection to influenza virus-challenged mice.					
31104946	7	11	theme	antibody	984:991	arg1	transfer					967:974	Passive transfer	959:974	Passive transfer of this antibody	959:991	Passive transfer of this antibody conferred Fc-dependent protection to influenza virus-challenged mice.					
31104946	2	12	theme	mechanisms	282:291	arg1	viruses					293:299	the mechanisms viruses	278:299	the mechanisms viruses	278:299	Antigenic variation including glycan introduction or removal is among the mechanisms viruses have evolved to escape host immunity.					
31104946	8	13	theme	preferred	1179:1187	arg1	s					1197:1197	preferred epitope(s)	1179:1198	preferred epitope(s)	1179:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	0	14	theme	Antigen	10:16	arg1	Engineering					18:28	Influenza Antigen Engineering	0:28	Influenza Antigen Engineering	0:28	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31104946	8	15	theme	B	1159:1159	arg1	responses					1166:1174	B cell responses	1159:1174	B cell responses to preferred epitope(s)	1159:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	6	16	theme	Structural	827:836	arg1	analyses					838:845	Structural analyses	827:845	Structural analyses	827:845	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	1	17	theme	adaptive	181:188	arg1	responses					197:205	a host's adaptive immune responses	172:205	a host's adaptive immune responses	172:205	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	0	18	theme	Influenza	0:8	arg1	Engineering					18:28	Influenza Antigen Engineering	0:28	Influenza Antigen Engineering	0:28	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31104946	4	19	theme	virus	581:585	arg1	HA					602:603	HA	602:603	HA	602:603	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	4	19	theme	virus	581:585	arg1	hemagglutinin					587:599	the influenza virus hemagglutinin	567:599	the influenza virus hemagglutinin (HA)	567:604	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	2	20	theme	host	324:327	arg1	immunity					329:336	host immunity	324:336	host immunity	324:336	Antigenic variation including glycan introduction or removal is among the mechanisms viruses have evolved to escape host immunity.					
31104946	1	21	theme	immune	190:195	arg1	responses					197:205	a host's adaptive immune responses	172:205	a host's adaptive immune responses	172:205	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	3	22	theme	protein	406:412	arg1	antigens					414:421	complex protein antigens	398:421	complex protein antigens	398:421	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	4	23	theme	influenza	571:579	arg1	HA					602:603	HA	602:603	HA	602:603	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	4	23	theme	influenza	571:579	arg1	hemagglutinin					587:599	the influenza virus hemagglutinin	567:599	the influenza virus hemagglutinin (HA)	567:604	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	8	24	theme	potential	1082:1090	arg1	implications					1092:1103	potential implications	1082:1103	potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s)	1082:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	4	25	theme	elicited	654:661	arg1	immunity					671:678	the elicited humoral immunity	650:678	the elicited humoral immunity	650:678	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	1	26	gly	glycoproteins	116:128	arg1	glycoproteins					116:128	Viral glycoproteins	110:128	Viral glycoproteins	110:128	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	4	27	theme	B	485:485	arg1	responses					492:500	B cell responses	485:500	B cell responses	485:500	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	1	28	theme	Viral	110:114	arg1	glycoproteins					116:128	Viral glycoproteins	110:128	Viral glycoproteins	110:128	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	3	29	theme	B	452:452	arg1	biology					459:465	B cell biology	452:465	B cell biology	452:465	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	5	30	dep	epitope-focused	777:791	arg1	restricted					806:815	restricted	806:815	restricted	806:815	We found that glycan addition changed the initially diverse antibody repertoire into an epitope-focused, genetically restricted response.					
31104946	3	31	from	immunodominance	379:393	arg1	antigens					414:421	complex protein antigens	398:421	complex protein antigens	398:421	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	8	32	theme	cell	1161:1164	arg1	responses					1166:1174	B cell responses	1159:1174	B cell responses to preferred epitope(s)	1159:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	4	33	theme	immunity	671:678	arg1	features					638:645	the molecular features	624:645	the molecular features of the elicited humoral immunity in mice	624:686	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	0	34	theme	Immune	38:43	arg1	Responses					45:53	Immune Responses	38:53	Immune Responses to a Subdominant but Broadly Protective Viral Epitope	38:107	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31104946	6	35	dep	subdominant	906:916	arg1	occluded					919:926	occluded	919:926	occluded	919:926	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	3	36	theme	cell	454:457	arg1	biology					459:465	B cell biology	452:465	B cell biology	452:465	Understanding how glycosylation affects immunodominance on complex protein antigens may help decipher underlying B cell biology.					
31104946	6	37	theme	subdominant	906:916	arg1	epitope					928:934	a previously subdominant, occluded epitope	893:934	a previously subdominant, occluded epitope at the head interface	893:956	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	4	38	theme	humoral	663:669	arg1	immunity					671:678	the elicited humoral immunity	650:678	the elicited humoral immunity	650:678	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	4	39	theme	cell	487:490	arg1	responses					492:500	B cell responses	485:500	B cell responses	485:500	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	2	40	theme	glycan	238:243	arg1	introduction					245:256	glycan introduction	238:256	glycan introduction	238:256	Antigenic variation including glycan introduction or removal is among the mechanisms viruses have evolved to escape host immunity.					
31104946	5	41	theme	diverse	741:747	arg1	repertoire					758:767	the initially diverse antibody repertoire	727:767	the initially diverse antibody repertoire	727:767	We found that glycan addition changed the initially diverse antibody repertoire into an epitope-focused, genetically restricted response.					
31104946	8	42	theme	epitope	1189:1195	arg1	s					1197:1197	preferred epitope(s)	1179:1198	preferred epitope(s)	1179:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	7	43	theme	Passive	959:965	arg1	transfer					967:974	Passive transfer	959:974	Passive transfer of this antibody	959:991	Passive transfer of this antibody conferred Fc-dependent protection to influenza virus-challenged mice.					
31104946	6	44	from	interface	948:956	arg1	epitope					928:934	a previously subdominant, occluded epitope	893:934	a previously subdominant, occluded epitope at the head interface	893:956	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	5	45	theme	glycan	703:708	arg1	addition					710:717	glycan addition	703:717	glycan addition	703:717	We found that glycan addition changed the initially diverse antibody repertoire into an epitope-focused, genetically restricted response.					
31104946	2	46	theme	Antigenic	208:216	arg1	variation					218:226	Antigenic variation	208:226	Antigenic variation including glycan introduction or removal	208:267	Antigenic variation including glycan introduction or removal is among the mechanisms viruses have evolved to escape host immunity.					
31104946	4	47	from	features	638:645	arg1	mice					683:686	mice	683:686	mice	683:686	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	4	48	theme	molecular	628:636	arg1	features					638:645	the molecular features	624:645	the molecular features of the elicited humoral immunity in mice	624:686	To determine how B cell responses can be altered by such modifications, we engineered glycans onto the influenza virus hemagglutinin (HA) and characterized the molecular features of the elicited humoral immunity in mice.					
31104946	1	49	theme	constant	140:147	arg1	surveillance					156:167	constant immune surveillance	140:167	constant immune surveillance by a host's adaptive immune responses	140:205	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	5	50	theme	epitope-focused	777:791	arg1	response					817:824	an epitope-focused, genetically restricted response	774:824	an epitope-focused, genetically restricted response	774:824	We found that glycan addition changed the initially diverse antibody repertoire into an epitope-focused, genetically restricted response.					
31104946	6	51	theme	gene	872:875	arg1	family					877:882	one antibody gene family	859:882	one antibody gene family	859:882	Structural analyses showed that one antibody gene family targeted a previously subdominant, occluded epitope at the head interface.					
31104946	1	52	theme	immune	149:154	arg1	surveillance					156:167	constant immune surveillance	140:167	constant immune surveillance by a host's adaptive immune responses	140:205	Viral glycoproteins are under constant immune surveillance by a host's adaptive immune responses.					
31104946	0	53	theme	Subdominant	60:70	arg1	Epitope					101:107	a Subdominant but Broadly Protective Viral Epitope	58:107	a Subdominant but Broadly Protective Viral Epitope	58:107	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31104946	8	54	contain	have	1077:1080	arg2	implications					1092:1103	potential implications	1082:1103	potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s)	1082:1198	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	8	54	contain	have	1077:1080	arg1	results					1069:1075	These results	1063:1075	These results	1063:1075	These results have potential implications for next-generation viral vaccines aimed at directing B cell responses to preferred epitope(s).					
31104946	7	55	theme	virus-challenged	1040:1055	arg1	mice					1057:1060	influenza virus-challenged mice	1030:1060	influenza virus-challenged mice	1030:1060	Passive transfer of this antibody conferred Fc-dependent protection to influenza virus-challenged mice.					
31104946	0	56	theme	Viral	95:99	arg1	Epitope					101:107	a Subdominant but Broadly Protective Viral Epitope	58:107	a Subdominant but Broadly Protective Viral Epitope	58:107	Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope.					
31446948	8	0	theme	cancer	1632:1637	arg1	n = 16					1646:1651	n = 16	1646:1651	n = 16	1646:1651	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	8	0	theme	cancer	1632:1637	arg1	group					1639:1643	lung cancer group	1627:1643	lung cancer group (n = 16)	1627:1652	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	7	1	theme	quantitative	1433:1444	arg1	accuracy					1446:1453	good quantitative accuracy	1428:1453	good quantitative accuracy	1428:1453	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	3	2	theme	novel	717:721	arg1	method					723:728	a novel method	715:728	a novel method	715:728	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	6	3	theme	biological	1249:1258	arg1	sample					1260:1265	biological sample	1249:1265	biological sample	1249:1265	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	7	4	theme	high	1459:1462	arg1	reproducibility					1464:1478	high reproducibility	1459:1478	high reproducibility	1459:1478	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	2	5	theme	dynamic	635:641	arg1	mass					643:646	broad dynamic mass and ion abundance range	629:670	mass	643:646	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	6	6	theme	same	1148:1151	arg1	composition					1161:1171	same glycome composition	1148:1171	same glycome composition	1148:1171	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	8	7	theme	clinical	1713:1720	arg1	applications					1722:1733	clinical applications	1713:1733	clinical applications	1713:1733	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	6	8	dep	composition	1161:1171	arg1	the					1144:1146	the	1144:1146	the	1144:1146	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	8	9	from	sample	1617:1622	arg1	glycans					1599:1605	the serum glycans	1589:1605	the serum glycans from each sample	1589:1622	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	4	10	theme	glycan	924:929	arg1	Glycan-RAIL					962:972	Glycan-RAIL	962:972	Glycan-RAIL	962:972	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	4	10	theme	glycan	924:929	arg1	reducing					931:938	glycan reducing and isotope labeling	924:959	reducing	931:938	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	0	11	theme	N-glycome	127:135	arg1	quantitation					111:122	reliable and simple quantitation	91:122	reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	91:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	2	12	theme	structural	593:602	arg1	heterogeneity					604:616	the natural glycan structural heterogeneity	574:616	the natural glycan structural heterogeneity	574:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	0	13	theme	biopharmaceuticals	307:324	arg1	biomarkers					273:282	disease biomarkers	265:282	disease biomarkers	265:282	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	0	13	theme	biopharmaceuticals	307:324	arg1	control					296:302	quality control	288:302	quality control of biopharmaceuticals	288:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	2	14	theme	natural	578:584	arg1	heterogeneity					604:616	the natural glycan structural heterogeneity	574:616	the natural glycan structural heterogeneity	574:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	8	15	used	used	1518:1521	arg2	method					1494:1499	this method	1489:1499	this method	1489:1499	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	5	16	theme	glycan	1042:1047	arg1	analyte					1049:1055	its corresponding glycan analyte	1024:1055	its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference	1024:1109	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	6	17	theme	similar	1177:1183	arg1	profile					1193:1199	similar glycome profile	1177:1199	similar glycome profile	1177:1199	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	2	18	theme	available	507:515	arg1	libraries					542:550	available glycan internal standard libraries	507:550	available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	507:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	3	19	theme	glycan	784:789	arg1	quantitation					791:802	glycan quantitation	784:802	glycan quantitation by MALDI-MS	784:814	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	0	20	theme	isotope	70:76	arg1	labeling					78:85	glycan reducing and isotope labeling	50:85	labeling	78:85	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	4	21	from	samples	873:879	arg1	N-glycome					851:859	N-glycome	851:859	N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL)	851:973	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	2	22	theme	libraries	542:550	arg1	lack					499:502	the lack	495:502	the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	495:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	5	23	theme	H/D	1091:1093	arg1	difference					1100:1109	H/D mass difference	1091:1109	H/D mass difference	1091:1109	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	2	24	theme	internal	524:531	arg1	libraries					542:550	available glycan internal standard libraries	507:550	available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	507:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	2	25	theme	ion	652:654	arg1	range					666:670	broad dynamic mass and ion abundance range	629:670	range	666:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	1	26	theme	internal	398:405	arg1	standards					407:415	exogenous isotopic labeling internal standards	370:415	exogenous isotopic labeling internal standards	370:415	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards is promising as it is simple and accurate.					
31446948	0	27	theme	disease	265:271	arg1	biomarkers					273:282	disease biomarkers	265:282	disease biomarkers	265:282	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	8	28	theme	lung	1540:1543	arg1	cancer					1545:1550	lung cancer specific glycan markers	1540:1574	lung cancer specific glycan markers	1540:1574	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	2	29	with	libraries	542:550	arg1	coverage					562:569	good coverage	557:569	good coverage of the natural glycan structural heterogeneity	557:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	7	30	theme	complex	1324:1330	arg1	glycans					1332:1338	single glycan standard and complex glycans	1297:1338	single glycan standard and complex glycans released from model glycoprotein and serum	1297:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	8	31	theme	specific	1552:1559	arg1	markers					1568:1574	specific glycan markers	1552:1574	lung cancer specific glycan markers	1540:1574	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	8	32	from	potential	1700:1708	arg1	applications					1722:1733	clinical applications	1713:1733	clinical applications	1713:1733	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	7	33	theme	standard	1311:1318	arg1	glycans					1332:1338	single glycan standard and complex glycans	1297:1338	single glycan standard and complex glycans released from model glycoprotein and serum	1297:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	4	34	theme	Bionic	817:822	arg1	Glycome					824:830	Bionic Glycome	817:830	Bionic Glycome	817:830	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	0	35	theme	internal	28:35	arg1	Glycome					17:23	Bionic Glycome	10:23	Bionic Glycome	10:23	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	0	35	theme	internal	28:35	arg1	standards					37:45	internal standards	28:45	internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	28:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	5	36	theme	mass	1004:1007	arg1	increment					1009:1017	3 Da mass increment	999:1017	3 Da mass increment	999:1017	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	7	37	theme	single	1297:1302	arg1	glycans					1332:1338	single glycan standard and complex glycans	1297:1338	single glycan standard and complex glycans released from model glycoprotein and serum	1297:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	8	38	theme	serum	1593:1597	arg1	glycans					1599:1605	the serum glycans	1589:1605	the serum glycans from each sample	1589:1622	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	8	39	dep	cancer	1545:1550	arg1	markers					1568:1574	specific glycan markers	1552:1574	lung cancer specific glycan markers	1540:1574	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	6	40	theme	glycome	1153:1159	arg1	composition					1161:1171	same glycome composition	1148:1171	same glycome composition	1148:1171	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	5	41	theme	bionic	981:986	arg1	glycan					988:993	Each bionic glycan	976:993	Each bionic glycan	976:993	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	5	42	contain	has	995:997	arg1	glycan					988:993	Each bionic glycan	976:993	Each bionic glycan	976:993	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	5	42	contain	has	995:997	arg2	increment					1009:1017	3 Da mass increment	999:1017	3 Da mass increment	999:1017	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	7	43	contain	has	1424:1426	arg2	accuracy					1446:1453	good quantitative accuracy	1428:1453	good quantitative accuracy	1428:1453	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	7	43	contain	has	1424:1426	arg1	method					1417:1422	the method	1413:1422	the method	1413:1422	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	7	43	contain	has	1424:1426	arg2	reproducibility					1464:1478	high reproducibility	1459:1478	high reproducibility	1459:1478	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	0	44	theme	simple	167:172	arg1	methods					189:195	methods	189:195	Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	157:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	4	45	theme	pooled	866:871	arg1	samples					873:879	pooled samples	866:879	pooled samples	866:879	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	6	46	contain	has	1140:1142	arg1	Glycome					1132:1138	Bionic Glycome	1125:1138	Bionic Glycome	1125:1138	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	6	46	contain	has	1140:1142	arg2	composition					1161:1171	same glycome composition	1148:1171	same glycome composition	1148:1171	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	6	46	contain	has	1140:1142	arg2	profile					1193:1199	similar glycome profile	1177:1199	similar glycome profile	1177:1199	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	0	47	theme	economical	178:187	arg1	methods					189:195	methods	189:195	Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	157:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	2	48	theme	heterogeneity	604:616	arg1	coverage					562:569	good coverage	557:569	good coverage of the natural glycan structural heterogeneity	557:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	2	49	theme	mass	643:646	arg1	lack					499:502	the lack	495:502	the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	495:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	7	50	theme	good	1428:1431	arg1	accuracy					1446:1453	good quantitative accuracy	1428:1453	good quantitative accuracy	1428:1453	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	2	51	with	range	666:670	arg1	coverage					562:569	good coverage	557:569	good coverage of the natural glycan structural heterogeneity	557:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	7	52	gly	glycoprotein	1360:1371	arg1	glycoprotein					1360:1371	model glycoprotein	1354:1371	model glycoprotein	1354:1371	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	6	53	with	composition	1161:1171	arg1	N-glycome					1219:1227	N-glycome	1219:1227	N-glycome	1219:1227	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	2	54	theme	broad	629:633	arg1	mass					643:646	broad dynamic mass and ion abundance range	629:670	mass	643:646	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	3	55	theme	Bionic	742:747	arg1	Glycome					749:755	'Bionic Glycome'	741:756	'Bionic Glycome'	741:756	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	3	55	theme	Bionic	742:747	arg1	standards					770:778	internal standards	761:778	internal standards for glycan quantitation by MALDI-MS	761:814	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	2	56	with	mass	643:646	arg1	coverage					562:569	good coverage	557:569	good coverage of the natural glycan structural heterogeneity	557:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	0	57	theme	quality	288:294	arg1	control					296:302	quality control	288:302	quality control of biopharmaceuticals	288:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	2	58	theme	glycan	586:591	arg1	heterogeneity					604:616	the natural glycan structural heterogeneity	574:616	the natural glycan structural heterogeneity	574:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	3	59	theme	internal	761:768	arg1	Glycome					749:755	'Bionic Glycome'	741:756	'Bionic Glycome'	741:756	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	3	59	theme	internal	761:768	arg1	standards					770:778	internal standards	761:778	internal standards for glycan quantitation by MALDI-MS	761:814	To overcome this limitation, we developed a novel method, providing 'Bionic Glycome' as internal standards for glycan quantitation by MALDI-MS.					
31446948	0	60	theme	Accurate	157:164	arg1	methods					189:195	methods	189:195	Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	157:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	5	61	theme	corresponding	1028:1040	arg1	analyte					1049:1055	its corresponding glycan analyte	1024:1055	its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference	1024:1109	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	7	62	theme	model	1354:1358	arg1	glycoprotein					1360:1371	model glycoprotein	1354:1371	model glycoprotein	1354:1371	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	6	63	from	profile	1193:1199	arg1	abundance					1204:1212	abundance	1204:1212	abundance	1204:1212	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	4	64	theme	reducing	931:938	arg1	step					916:919	one step	912:919	one step of glycan reducing and isotope labeling (Glycan-RAIL)	912:973	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	1	65	theme	isotopic	380:387	arg1	standards					407:415	exogenous isotopic labeling internal standards	370:415	exogenous isotopic labeling internal standards	370:415	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards is promising as it is simple and accurate.					
31446948	6	66	with	profile	1193:1199	arg1	N-glycome					1219:1227	N-glycome	1219:1227	N-glycome	1219:1227	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	0	67	theme	reliable	91:98	arg1	quantitation					111:122	reliable and simple quantitation	91:122	reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	91:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	1	68	theme	exogenous	370:378	arg1	standards					407:415	exogenous isotopic labeling internal standards	370:415	exogenous isotopic labeling internal standards	370:415	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards is promising as it is simple and accurate.					
31446948	2	69	theme	standard	533:540	arg1	libraries					542:550	available glycan internal standard libraries	507:550	available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	507:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	2	70	theme	abundance	656:664	arg1	range					666:670	broad dynamic mass and ion abundance range	629:670	range	666:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	6	71	from	composition	1161:1171	arg1	abundance					1204:1212	abundance	1204:1212	abundance	1204:1212	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	0	72	theme	simple	104:109	arg1	quantitation					111:122	reliable and simple quantitation	91:122	reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	91:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	1	73	theme	labeling	389:396	arg1	standards					407:415	exogenous isotopic labeling internal standards	370:415	exogenous isotopic labeling internal standards	370:415	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards is promising as it is simple and accurate.					
31446948	7	74	theme	glycans	1332:1338	arg1	investigation					1280:1292	the investigation	1276:1292	the investigation of single glycan standard and complex glycans released from model glycoprotein and serum	1276:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	2	75	theme	glycan	517:522	arg1	libraries					542:550	available glycan internal standard libraries	507:550	available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range	507:670	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	8	76	theme	cancer	1545:1550	arg1	discovery					1527:1535	discovery	1527:1535	discovery of lung cancer specific glycan markers	1527:1574	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	4	77	theme	labeling	952:959	arg1	step					916:919	one step	912:919	one step of glycan reducing and isotope labeling (Glycan-RAIL)	912:973	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	0	78	theme	Bionic	10:15	arg1	Glycome					17:23	Bionic Glycome	10:23	Bionic Glycome	10:23	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	0	78	theme	Bionic	10:15	arg1	standards					37:45	internal standards	28:45	internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals	28:324	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	5	79	theme	mass	1095:1098	arg1	difference					1100:1109	H/D mass difference	1091:1109	H/D mass difference	1091:1109	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	2	80	theme	good	557:560	arg1	coverage					562:569	good coverage	557:569	good coverage of the natural glycan structural heterogeneity	557:616	However, it is largely hampered by the lack of available glycan internal standard libraries with good coverage of the natural glycan structural heterogeneity as well as broad dynamic mass and ion abundance range.					
31446948	8	81	theme	glycan	1561:1566	arg1	markers					1568:1574	specific glycan markers	1552:1574	lung cancer specific glycan markers	1540:1574	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	7	82	theme	glycan	1304:1309	arg1	glycans					1332:1338	single glycan standard and complex glycans	1297:1338	single glycan standard and complex glycans released from model glycoprotein and serum	1297:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	5	83	theme	3 Da	999:1002	arg1	increment					1009:1017	3 Da mass increment	999:1017	3 Da mass increment	999:1017	Each bionic glycan has 3 Da mass increment over its corresponding glycan analyte based on hemiacetals/alditols and H/D mass difference.					
31446948	6	84	theme	glycome	1185:1191	arg1	profile					1193:1199	similar glycome profile	1177:1199	similar glycome profile	1177:1199	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	4	85	theme	isotope	944:950	arg1	labeling					952:959	glycan reducing and isotope labeling	924:959	labeling	952:959	Bionic Glycome was produced using N-glycome from pooled samples to be analyzed as substrate by one step of glycan reducing and isotope labeling (Glycan-RAIL).					
31446948	0	86	theme	glycan	50:55	arg1	reducing					57:64	glycan reducing and isotope labeling	50:85	reducing	57:64	Providing Bionic Glycome as internal standards by glycan reducing and isotope labeling for reliable and simple quantitation of N-glycome based on MALDI- MS. Accurate, simple and economical methods for quantifying N-glycans are continuously required for discovering disease biomarkers and quality control of biopharmaceuticals.					
31446948	1	87	theme	Quantitative	327:338	arg1	N-glycomics					340:350	Quantitative N-glycomics	327:350	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards	327:415	Quantitative N-glycomics based on MS using exogenous isotopic labeling internal standards is promising as it is simple and accurate.					
31446948	6	88	theme	Bionic	1125:1130	arg1	Glycome					1132:1138	Bionic Glycome	1125:1138	Bionic Glycome	1125:1138	In addition, Bionic Glycome has the same glycome composition and similar glycome profile in abundance with N-glycome to be analyzed from biological sample.					
31446948	7	89	attach	released	1340:1347	arg1	glycoprotein					1360:1371	model glycoprotein	1354:1371	model glycoprotein	1354:1371	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	7	89	attach	released	1340:1347	arg2	glycans					1332:1338	single glycan standard and complex glycans	1297:1338	single glycan standard and complex glycans released from model glycoprotein and serum	1297:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	7	89	attach	released	1340:1347	arg1	serum					1377:1381	serum	1377:1381	serum	1377:1381	Through the investigation of single glycan standard and complex glycans released from model glycoprotein and serum, the results demonstrate that the method has good quantitative accuracy and high reproducibility.					
31446948	8	90	theme	lung	1627:1630	arg1	cancer					1632:1637	lung cancer	1627:1637	lung cancer group (n = 16)	1627:1652	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	8	91	theme	healthy	1658:1664	arg1	n = 16					1676:1681	n = 16	1676:1681	n = 16	1676:1681	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
31446948	8	91	theme	healthy	1658:1664	arg1	controls					1666:1673	healthy controls	1658:1673	healthy controls (n = 16)	1658:1682	Lastly, this method was successfully used for discovery of lung cancer specific glycan markers by comparing the serum glycans from each sample in lung cancer group (n = 16) and healthy controls (n = 16), indicating its potential in clinical applications.					
30018219	7	0	theme	expression	1229:1238	arg1	irrespective					1194:1205	irrespective	1194:1205	irrespective	1194:1205	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	6	1	theme	Notch1	894:899	arg1	repeats					905:911	recombinant Notch1 EGF repeats	882:911	recombinant Notch1 EGF repeats isolated from HEK293T cells	882:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	1	2	theme	modification	156:167	arg1	O-GlcNAc					127:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	2	theme	modification	156:167	arg1	class					147:151	a novel class	139:151	a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	139:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	7	3	theme	EGF20	1066:1070	arg1	domains					1072:1078	EGF20 domains	1066:1078	EGF20 domains	1066:1078	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	4	4	theme	model	588:592	arg1	substrates					594:603	model substrates	588:603	model substrates	588:603	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	5	5	mod	modified	763:770	arg3	form					790:793	an elongated form	777:793	an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues	777:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	5	5	mod	modified	763:770	arg1	majority					734:741	the majority	730:741	the majority of the proteins	730:757	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	0	6	from	Divergence	11:20	arg1	Glycans					34:40	O-GlcNAc Glycans	25:40	O-GlcNAc Glycans	25:40	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	7	7	theme	many	1140:1143	arg1	domains					1151:1157	many other domains	1140:1157	many other domains	1140:1157	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	0	8	theme	Notch1	105:110	arg1	Repeats					84:90	Epidermal Growth Factor-like Repeats	55:90	Epidermal Growth Factor-like Repeats of Mammalian Notch1	55:110	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	5	9	theme	Drosophila	672:681	arg1	EGF20					683:687	Drosophila EGF20	672:687	Drosophila EGF20 expressed in HEK293T cells	672:714	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	8	10	theme	receptors	1362:1370	arg1	function					1344:1351	function	1344:1351	function	1344:1351	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	8	10	theme	receptors	1362:1370	arg1	structure					1330:1338	structure	1330:1338	structure	1330:1338	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	3	11	theme	mammalian	401:409	arg1	cells					420:424	mammalian cultured cells	401:424	mammalian cultured cells	401:424	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	0	12	theme	Mammalian	95:103	arg1	Notch1					105:110	Mammalian Notch1	95:110	Mammalian Notch1	95:110	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	5	13	theme	EGF20	683:687	arg1	analysis					660:667	The analysis	656:667	The analysis of Drosophila EGF20 expressed in HEK293T cells	656:714	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	8	14	theme	Notch	1356:1360	arg1	receptors					1362:1370	Notch receptors	1356:1370	Notch receptors	1356:1370	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	6	15	theme	EGF	1012:1014	arg1	domains					1016:1022	the different EGF domains	998:1022	the different EGF domains	998:1022	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	5	16	theme	proteins	750:757	arg1	majority					734:741	the majority	730:741	the majority of the proteins	730:757	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	7	17	theme	Notch1	1050:1055	arg1	EGF2					1057:1060	Notch1 EGF2	1050:1060	Notch1 EGF2	1050:1060	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	7	18	theme	glycan	1116:1121	arg1	forms					1103:1107	the extended forms	1090:1107	the extended forms of the glycan	1090:1121	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	5	19	theme	acid	854:857	arg1	residues					859:866	terminal galactose or sialic acid residues	825:866	residues	859:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	3	20	theme	subsequent	440:449	arg1	glycosylation					451:463	subsequent glycosylation	440:463	subsequent glycosylation	440:463	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	3	21	theme	Previous	353:360	arg1	studies					362:368	Previous studies	353:368	Previous studies	353:368	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	3	22	from	O-GlcNAc	389:396	arg1	cells					420:424	mammalian cultured cells	401:424	mammalian cultured cells	401:424	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	4	23	theme	mouse	609:613	arg1	repeats					626:632	mouse Notch1 EGF repeats	609:632	mouse Notch1 EGF repeats	609:632	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	1	24	theme	epidermal	182:190	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	24	theme	epidermal	182:190	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	4	25	theme	Drosophila	568:577	arg1	EGF20					579:583	Drosophila EGF20	568:583	Drosophila EGF20	568:583	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	2	26	theme	Notch	311:315	arg1	signaling					317:325	ligand-mediated Notch signaling	295:325	ligand-mediated Notch signaling	295:325	In mammals, EOGT is required for ligand-mediated Notch signaling for vascular development.					
30018219	1	27	theme	growth	192:197	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	27	theme	growth	192:197	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	0	28	theme	Structural	0:9	arg1	Divergence					11:20	Structural Divergence	0:20	Structural Divergence in O-GlcNAc Glycans	0:40	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	6	29	theme	different	1002:1010	arg1	domains					1016:1022	the different EGF domains	998:1022	the different EGF domains	998:1022	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	7	30	from	O-GlcNAc	1128:1135	arg1	domains					1151:1157	many other domains	1140:1157	many other domains	1140:1157	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	2	31	theme	ligand-mediated	295:309	arg1	signaling					317:325	ligand-mediated Notch signaling	295:325	ligand-mediated Notch signaling	295:325	In mammals, EOGT is required for ligand-mediated Notch signaling for vascular development.					
30018219	1	32	theme	factor-like	199:209	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	32	theme	factor-like	199:209	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	0	33	theme	O-GlcNAc	25:32	arg1	Glycans					34:40	O-GlcNAc Glycans	25:40	O-GlcNAc Glycans	25:40	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	5	34	theme	terminal	825:832	arg1	galactose					834:842	terminal galactose or sialic acid residues	825:866	galactose	834:842	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	7	35	theme	extended	1094:1101	arg1	forms					1103:1107	the extended forms	1090:1107	the extended forms of the glycan	1090:1121	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	5	36	theme	O-GlcNAc	798:805	arg1	glycan					807:812	O-GlcNAc glycan	798:812	O-GlcNAc glycan comprising terminal galactose or sialic acid residues	798:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	7	37	contain	contained	1080:1088	arg2	forms					1103:1107	the extended forms	1090:1107	the extended forms of the glycan	1090:1121	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	7	37	contain	contained	1080:1088	arg1	majority					1038:1045	the majority	1034:1045	the majority of Notch1 EGF2 and EGF20 domains	1034:1078	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	5	38	theme	sialic	847:852	arg1	residues					859:866	terminal galactose or sialic acid residues	825:866	residues	859:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	4	39	theme	EGF20	579:583	arg1	glycans					557:563	the O-GlcNAc glycans	544:563	the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats	544:632	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	7	40	theme	EGF2	1057:1060	arg1	majority					1038:1045	the majority	1034:1045	the majority of Notch1 EGF2 and EGF20 domains	1034:1078	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	6	41	theme	recombinant	882:892	arg1	repeats					905:911	recombinant Notch1 EGF repeats	882:911	recombinant Notch1 EGF repeats isolated from HEK293T cells	882:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	1	42	theme	Extracellular	113:125	arg1	O-GlcNAc					127:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	42	theme	Extracellular	113:125	arg1	class					147:151	a novel class	139:151	a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	139:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	43	theme	EGF	212:214	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	43	theme	EGF	212:214	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	3	44	theme	regulation	504:513	arg1	layers					494:499	additional layers	483:499	additional layers of regulation	483:513	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	7	45	theme	irrespective	1194:1205	arg1	monosaccharide					1179:1192	a monosaccharide	1177:1192	a monosaccharide irrespective of the exogenous EOGT expression	1177:1238	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	6	46	theme	glycans	984:990	arg1	divergence					961:970	structural divergence	950:970	structural divergence of O-GlcNAc glycans among the different EGF domains	950:1022	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	7	47	theme	other	1145:1149	arg1	domains					1151:1157	many other domains	1140:1157	many other domains	1140:1157	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	6	48	theme	O-GlcNAc	975:982	arg1	glycans					984:990	O-GlcNAc glycans	975:990	O-GlcNAc glycans	975:990	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	5	49	theme	elongated	780:788	arg1	form					790:793	an elongated form	777:793	an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues	777:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	8	50	theme	O-GlcNAc	1290:1297	arg1	monosaccharides					1299:1313	O-GlcNAc monosaccharides	1290:1313	O-GlcNAc monosaccharides	1290:1313	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	1	51	theme	-domain	216:222	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	51	theme	-domain	216:222	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	0	52	theme	Epidermal	55:63	arg1	Repeats					84:90	Epidermal Growth Factor-like Repeats	55:90	Epidermal Growth Factor-like Repeats of Mammalian Notch1	55:110	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	7	53	theme	exogenous	1214:1222	arg1	expression					1229:1238	the exogenous EOGT expression	1210:1238	the exogenous EOGT expression	1210:1238	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	5	54	theme	glycan	807:812	arg1	form					790:793	an elongated form	777:793	an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues	777:866	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	6	55	theme	EGF	901:903	arg1	repeats					905:911	recombinant Notch1 EGF repeats	882:911	recombinant Notch1 EGF repeats isolated from HEK293T cells	882:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	3	56	theme	cultured	411:418	arg1	cells					420:424	mammalian cultured cells	401:424	mammalian cultured cells	401:424	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	4	57	theme	O-GlcNAc	548:555	arg1	glycans					557:563	the O-GlcNAc glycans	544:563	the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats	544:632	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	7	58	theme	domains	1072:1078	arg1	majority					1038:1045	the majority	1034:1045	the majority of Notch1 EGF2 and EGF20 domains	1034:1078	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	6	59	theme	HEK293T	927:933	arg1	cells					935:939	HEK293T cells	927:939	HEK293T cells	927:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	8	60	dep	structure	1330:1338	arg1	the					1326:1328	the	1326:1328	the	1326:1328	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	7	61	theme	EOGT	1224:1227	arg1	expression					1229:1238	the exogenous EOGT expression	1210:1238	the exogenous EOGT expression	1210:1238	Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression.					
30018219	2	62	theme	vascular	331:338	arg1	development					340:350	vascular development	331:350	vascular development	331:350	In mammals, EOGT is required for ligand-mediated Notch signaling for vascular development.					
30018219	6	63	theme	structural	950:959	arg1	divergence					961:970	structural divergence	950:970	structural divergence of O-GlcNAc glycans among the different EGF domains	950:1022	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	5	64	theme	HEK293T	702:708	arg1	cells					710:714	HEK293T cells	702:714	HEK293T cells	702:714	The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues.					
30018219	3	65	theme	additional	483:492	arg1	layers					494:499	additional layers	483:499	additional layers of regulation	483:513	Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation.					
30018219	1	66	theme	specific	224:231	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	66	theme	specific	224:231	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	67	theme	novel	141:145	arg1	O-GlcNAc					127:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc	113:134	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	67	theme	novel	141:145	arg1	class					147:151	a novel class	139:151	a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	139:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	68	theme	O-GlcNAc	233:240	arg1	transferase					242:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase	182:252	epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT)	182:259	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	1	68	theme	O-GlcNAc	233:240	arg1	EOGT					255:258	EOGT	255:258	EOGT	255:258	Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT).					
30018219	0	69	theme	Factor-like	72:82	arg1	Repeats					84:90	Epidermal Growth Factor-like Repeats	55:90	Epidermal Growth Factor-like Repeats of Mammalian Notch1	55:110	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	6	70	attach	isolated	913:920	arg1	cells					935:939	HEK293T cells	927:939	HEK293T cells	927:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	6	70	attach	isolated	913:920	arg2	repeats					905:911	recombinant Notch1 EGF repeats	882:911	recombinant Notch1 EGF repeats isolated from HEK293T cells	882:939	In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains.					
30018219	0	71	theme	Growth	65:70	arg1	Repeats					84:90	Epidermal Growth Factor-like Repeats	55:90	Epidermal Growth Factor-like Repeats of Mammalian Notch1	55:110	Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1.					
30018219	4	72	theme	Notch1	615:620	arg1	repeats					626:632	mouse Notch1 EGF repeats	609:632	mouse Notch1 EGF repeats	609:632	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
30018219	8	73	theme	monosaccharides	1299:1313	arg1	array					1281:1285	an array	1278:1285	an array of O-GlcNAc monosaccharides	1278:1313	Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.					
30018219	4	74	theme	EGF	622:624	arg1	repeats					626:632	mouse Notch1 EGF repeats	609:632	mouse Notch1 EGF repeats	609:632	This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry.					
31145522	3	0	theme	radical	631:637	arg1	prostatectomy					639:651	radical prostatectomy	631:651	radical prostatectomy in Australia (preop-PSA cohort)	631:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	8	1	theme	family	1621:1626	arg1	history					1628:1634	no family history information	1618:1646	no family history information prior to biopsy	1618:1662	Limitations include limited sample size, retrospective nature, and no family history information prior to biopsy.					
31145522	9	2	theme	LacdiNAc-glycosylated	1665:1685	arg1	PSA					1687:1689	LacdiNAc-glycosylated PSA	1665:1689	LacdiNAc-glycosylated PSA	1665:1689	LacdiNAc-glycosylated PSA is significantly better than the conventional PSA test in identifying patients with CSPC.					
31145522	2	3	theme	prostate-specific	307:323	arg1	LDN-PSA					334:340	LDN-PSA	334:340	LDN-PSA	334:340	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	3	theme	prostate-specific	307:323	arg1	antigen					325:331	LacdiNAc-glycosylated prostate-specific antigen	285:331	LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA)	285:341	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	4	dep	determined	255:264	arg1	identify					197:204	identify	197:204	To identify clinically significant prostate cancer (CSPC)	194:250	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	5	gly	LacdiNAc-glycosylated	285:305	arg1	LDN-PSA					334:340	LDN-PSA	334:340	LDN-PSA	334:340	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	5	gly	LacdiNAc-glycosylated	285:305	arg1	antigen					325:331	LacdiNAc-glycosylated prostate-specific antigen	285:331	LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA)	285:341	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	4	6	theme	curve	797:801	arg1	analyses					803:810	decision curve analyses	788:810	decision curve analyses	788:810	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
31145522	10	7	theme	Structure	1880:1888	arg1	Change					1890:1895	Carbohydrate Structure Change	1867:1895	Carbohydrate Structure Change in Urological Disease"	1867:1918	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	6	8	theme	decision	1097:1104	arg1	analysis					1112:1119	The decision curve analysis	1093:1119	The decision curve analysis	1093:1119	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	3	9	theme	academic	523:530	arg1	clinics					540:546	3 academic urology clinics	521:546	3 academic urology clinics	521:546	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	6	10	dep	adding	1167:1172	arg1	tPSA					1254:1257	tPSA	1254:1257	tPSA	1254:1257	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	10	dep	adding	1167:1172	arg1	status					1246:1251	digital rectal examination status	1219:1251	digital rectal examination status	1219:1251	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	10	dep	adding	1167:1172	arg1	age					1214:1216	age	1214:1216	age	1214:1216	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	10	dep	adding	1167:1172	arg1	PSA					1268:1270	F/T PSA	1264:1270	F/T PSA	1264:1270	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	9	11	theme	PSA	1737:1739	arg1	test					1741:1744	the conventional PSA test	1720:1744	the conventional PSA test	1720:1744	LacdiNAc-glycosylated PSA is significantly better than the conventional PSA test in identifying patients with CSPC.					
31145522	3	12	from	prostatectomy	639:651	arg1	Australia					656:664	Australia	656:664	Australia (preop-PSA cohort)	656:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	12	from	prostatectomy	639:651	arg1	cohort					677:682	preop-PSA cohort	667:682	preop-PSA cohort	667:682	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	6	13	theme	[base	1377:1381	arg1	model					1383:1387	[base model	1377:1387	2.23% [base model	1371:1387	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	3	14	theme	Pbx	569:571	arg1	cohort					573:578	Pbx cohort	569:578	Pbx cohort	569:578	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	14	theme	Pbx	569:571	arg1	Japan					551:555	Japan	551:555	Japan	551:555	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	8	15	theme	limited	1571:1577	arg1	size					1586:1589	limited sample size	1571:1589	limited sample size	1571:1589	Limitations include limited sample size, retrospective nature, and no family history information prior to biopsy.					
31145522	5	16	dep	PSA	1064:1066	arg1	AUC					1069:1071	AUC 0.661	1069:1077	AUC 0.661	1069:1077	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	5	16	dep	PSA	1064:1066	arg1	P < 0.0001					1080:1089	P < 0.0001	1080:1089	P < 0.0001	1080:1089	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	0	17	theme	cancer	96:101	arg1	detection					103:111	prostate cancer detection	87:111	prostate cancer detection	87:111	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	5	18	theme	Pbx	841:843	arg1	cohort					845:850	the Pbx cohort	837:850	the Pbx cohort	837:850	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	10	19	theme	ethics	1812:1817	arg1	committee					1819:1827	the ethics committee	1808:1827	the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195)	1808:1942	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	8	20	theme	retrospective	1592:1604	arg1	nature					1606:1611	retrospective nature	1592:1611	retrospective nature	1592:1611	Limitations include limited sample size, retrospective nature, and no family history information prior to biopsy.					
31145522	5	21	theme	better	897:902	arg1	performance					913:923	significantly better clinical performance	883:923	significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001)	883:1090	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	7	22	theme	tumor	1459:1463	arg1	volume					1465:1470	tumor volume	1459:1470	tumor volume	1459:1470	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	8	23	dep	history	1628:1634	arg1	information					1636:1646	information	1636:1646	no family history information prior to biopsy	1618:1662	Limitations include limited sample size, retrospective nature, and no family history information prior to biopsy.					
31145522	7	24	dep	pT3	1514:1516	arg1	pathological					1519:1530	pathological	1519:1530	pathological	1519:1530	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	10	25	dep	institution	1837:1847	arg1	Study					1855:1859	The Study	1851:1859	The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195	1851:1941	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	0	26	theme	Clinical	0:7	arg1	significance					9:20	Clinical significance	0:20	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.	0:112	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	3	27	theme	PSA/tPSA	461:468	arg1	values					480:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values	425:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort)	425:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	5	28	theme	F/T	1060:1062	arg1	PSA					1064:1066	F/T PSA	1060:1066	F/T PSA (AUC 0.661, P < 0.0001)	1060:1090	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	1	29	theme	unnecessary	124:134	arg1	Pbx					155:157	Pbx	155:157	Pbx	155:157	To reduce unnecessary prostate biopsies (Pbx), better discrimination is needed.					
31145522	1	29	theme	unnecessary	124:134	arg1	biopsies					145:152	unnecessary prostate biopsies	124:152	unnecessary prostate biopsies (Pbx)	124:158	To reduce unnecessary prostate biopsies (Pbx), better discrimination is needed.					
31145522	3	30	theme	total	434:438	arg1	tPSA					445:448	tPSA	445:448	tPSA	445:448	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	30	theme	total	434:438	arg1	PSA					440:442	total PSA	434:442	total PSA (tPSA)	434:449	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	5	31	dep	tPSA	1026:1029	arg1	P < 0.0001					1043:1052	P < 0.0001	1043:1052	P < 0.0001	1043:1052	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	5	31	dep	tPSA	1026:1029	arg1	AUC					1032:1034	AUC 0.712	1032:1040	AUC 0.712	1032:1040	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	3	32	theme	PC	592:593	arg1	patients					595:602	174 PC patients	588:602	174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort)	588:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	10	33	from	Change	1890:1895	arg1	"					1918:1918	Urological Disease"	1900:1918	Urological Disease"	1900:1918	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	6	34	theme	examination	1234:1244	arg1	status					1246:1251	digital rectal examination status	1219:1251	digital rectal examination status	1219:1251	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	0	35	theme	LacdiNAc-glycosylated	29:49	arg1	antigen					69:75	the LacdiNAc-glycosylated prostate-specific antigen	25:75	the LacdiNAc-glycosylated prostate-specific antigen assay	25:81	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	3	36	theme	LDN-PSA	425:431	arg1	values					480:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values	425:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort)	425:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	2	37	theme	prostate	369:376	arg1	LDN-PSAD					386:393	LDN-PSAD	386:393	LDN-PSAD	386:393	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	37	theme	prostate	369:376	arg1	volume					378:383	prostate volume	369:383	prostate volume (LDN-PSAD)	369:394	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	0	38	theme	antigen	69:75	arg1	assay					77:81	the LacdiNAc-glycosylated prostate-specific antigen assay	25:81	the LacdiNAc-glycosylated prostate-specific antigen assay	25:81	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	2	39	theme	significant	217:227	arg1	CSPC					246:249	CSPC	246:249	CSPC	246:249	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	39	theme	significant	217:227	arg1	cancer					238:243	clinically significant prostate cancer	206:243	clinically significant prostate cancer (CSPC)	206:250	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	3	40	theme	F/T	471:473	arg1	PSA/tPSA					461:468	free PSA/tPSA	456:468	free PSA/tPSA (F/T PSA)	456:478	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	40	theme	F/T	471:473	arg1	PSA					475:477	F/T PSA	471:477	F/T PSA	471:477	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	10	41	theme	institution	1837:1847	arg1	committee					1819:1827	the ethics committee	1808:1827	the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195)	1808:1942	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	4	42	theme	operating	746:754	arg1	characteristics					756:770	the receiver operating characteristics	733:770	the receiver operating characteristics curve (AUC)	733:782	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
31145522	0	43	theme	assay	77:81	arg1	significance					9:20	Clinical significance	0:20	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.	0:112	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	10	44	theme	Carbohydrate	1867:1878	arg1	Change					1890:1895	Carbohydrate Structure Change	1867:1895	Carbohydrate Structure Change in Urological Disease"	1867:1918	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	5	45	dep	PSAD	996:999	arg1	P < 0.0001					1013:1022	P < 0.0001	1013:1022	P < 0.0001	1013:1022	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	5	45	dep	PSAD	996:999	arg1	AUC					1002:1004	AUC 0.809	1002:1010	AUC 0.809	1002:1010	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	6	46	theme	risk	1141:1144	arg1	threshold					1146:1154	a risk threshold	1139:1154	a risk threshold of 20%	1139:1161	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	9	47	gly	LacdiNAc-glycosylated	1665:1685	arg1	PSA					1687:1689	LacdiNAc-glycosylated PSA	1665:1689	LacdiNAc-glycosylated PSA	1665:1689	LacdiNAc-glycosylated PSA is significantly better than the conventional PSA test in identifying patients with CSPC.					
31145522	10	48	dep	Study	1855:1859	arg1	no.					1930:1932	approval no. 2014-195	1921:1941	The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195	1851:1941	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	2	49	theme	antigen	325:331	arg1	performance					270:280	the performance	266:280	the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD)	266:394	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	6	50	theme	F/T	1264:1266	arg1	PSA					1268:1270	F/T PSA	1264:1270	F/T PSA	1264:1270	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	51	dep	CSPC	1331:1334	arg1	%					1375:1375	2.23%	1371:1375	2.23% [base model	1371:1387	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	51	dep	CSPC	1331:1334	arg1	%					1341:1341	9.89%	1337:1341	9.89%	1337:1341	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	51	dep	CSPC	1331:1334	arg1	%					1352:1352	18.11%	1347:1352	18.11%	1347:1352	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	10	52	theme	Urological	1900:1909	arg1	"					1918:1918	Urological Disease"	1900:1918	Urological Disease"	1900:1918	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	2	53	theme	LacdiNAc-glycosylated	285:305	arg1	LDN-PSA					334:340	LDN-PSA	334:340	LDN-PSA	334:340	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	53	theme	LacdiNAc-glycosylated	285:305	arg1	antigen					325:331	LacdiNAc-glycosylated prostate-specific antigen	285:331	LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA)	285:341	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	6	54	theme	digital	1219:1225	arg1	examination					1234:1244	digital rectal examination	1219:1244	digital rectal examination status	1219:1251	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	7	55	theme	Gleason	1532:1538	arg1	score ≥ 7					1540:1548	pT3, pathological Gleason score ≥ 7	1514:1548	pT3, pathological Gleason score ≥ 7	1514:1548	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	3	56	theme	preop-PSA	667:675	arg1	Australia					656:664	Australia	656:664	Australia (preop-PSA cohort)	656:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	56	theme	preop-PSA	667:675	arg1	cohort					677:682	preop-PSA cohort	667:682	preop-PSA cohort	667:682	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	6	57	theme	curve	1106:1110	arg1	analysis					1112:1119	The decision curve analysis	1093:1119	The decision curve analysis	1093:1119	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	3	58	theme	urology	532:538	arg1	clinics					540:546	3 academic urology clinics	521:546	3 academic urology clinics	521:546	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	7	59	theme	preop-PSA	1399:1407	arg1	cohort					1409:1414	the preop-PSA cohort	1395:1414	the preop-PSA cohort	1395:1414	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	4	60	theme	decision	788:795	arg1	analyses					803:810	decision curve analyses	788:810	decision curve analyses	788:810	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
31145522	10	61	theme	approval	1921:1928	arg1	no.					1930:1932	approval no. 2014-195	1921:1941	The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195	1851:1941	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	5	62	dep	LDN-PSA	963:969	arg1	P = 0.0024					983:992	P = 0.0024	983:992	P = 0.0024	983:992	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	5	62	dep	LDN-PSA	963:969	arg1	AUC					972:974	AUC 0.827	972:980	AUC 0.827	972:980	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	3	63	from	values	480:485	arg1	men					494:496	718 men	490:496	718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort)	490:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	9	64	theme	conventional	1724:1735	arg1	test					1741:1744	the conventional PSA test	1720:1744	the conventional PSA test	1720:1744	LacdiNAc-glycosylated PSA is significantly better than the conventional PSA test in identifying patients with CSPC.					
31145522	6	65	theme	biopsies	1306:1313	arg1	avoidance					1283:1291	avoidance	1283:1291	avoidance of even more biopsies	1283:1313	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	6	66	theme	%	1161:1161	arg1	threshold					1146:1154	a risk threshold	1139:1154	a risk threshold of 20%	1139:1161	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	1	67	theme	prostate	136:143	arg1	Pbx					155:157	Pbx	155:157	Pbx	155:157	To reduce unnecessary prostate biopsies (Pbx), better discrimination is needed.					
31145522	1	67	theme	prostate	136:143	arg1	biopsies					145:152	unnecessary prostate biopsies	124:152	unnecessary prostate biopsies (Pbx)	124:158	To reduce unnecessary prostate biopsies (Pbx), better discrimination is needed.					
31145522	7	68	theme	pT3	1514:1516	arg1	score ≥ 7					1540:1548	pT3, pathological Gleason score ≥ 7	1514:1548	pT3, pathological Gleason score ≥ 7	1514:1548	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	0	69	theme	prostate	87:94	arg1	detection					103:111	prostate cancer detection	87:111	prostate cancer detection	87:111	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	1	70	theme	better	161:166	arg1	discrimination					168:181	better discrimination	161:181	better discrimination	161:181	To reduce unnecessary prostate biopsies (Pbx), better discrimination is needed.					
31145522	3	71	from	Pbx	514:516	arg1	clinics					540:546	3 academic urology clinics	521:546	3 academic urology clinics	521:546	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	9	72	with	patients	1761:1768	arg1	CSPC					1775:1778	CSPC	1775:1778	CSPC	1775:1778	LacdiNAc-glycosylated PSA is significantly better than the conventional PSA test in identifying patients with CSPC.					
31145522	6	73	theme	base	1202:1205	arg1	model					1207:1211	the base model	1198:1211	the base model	1198:1211	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	8	74	theme	sample	1579:1584	arg1	size					1586:1589	limited sample size	1571:1589	limited sample size	1571:1589	Limitations include limited sample size, retrospective nature, and no family history information prior to biopsy.					
31145522	6	75	dep	%	1375:1375	arg1	model					1383:1387	[base model	1377:1387	2.23% [base model	1371:1387	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	5	76	theme	clinical	904:911	arg1	performance					913:923	significantly better clinical performance	883:923	significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001)	883:1090	In the Pbx cohort, LDN-PSAD (AUC 0.860) provided significantly better clinical performance for discriminating CSPC compared with LDN-PSA (AUC 0.827, P = 0.0024), PSAD (AUC 0.809, P < 0.0001), tPSA (AUC 0.712, P < 0.0001), and F/T PSA (AUC 0.661, P < 0.0001).					
31145522	10	77	theme	Disease	1911:1917	arg1	"					1918:1918	Urological Disease"	1900:1918	Urological Disease"	1900:1918	This study was approved by the ethics committee of each institution ("The Study about Carbohydrate Structure Change in Urological Disease"; approval no. 2014-195).					
31145522	0	78	theme	prostate-specific	51:67	arg1	antigen					69:75	the LacdiNAc-glycosylated prostate-specific antigen	25:75	the LacdiNAc-glycosylated prostate-specific antigen assay	25:81	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	7	79	theme	LDN-PSA	1417:1423	arg1	values					1425:1430	LDN-PSA values	1417:1430	LDN-PSA values	1417:1430	In the preop-PSA cohort, LDN-PSA values positively correlated with tumor volume and tPSA and were significantly higher in pT3, pathological Gleason score ≥ 7.					
31145522	3	80	theme	PSA	440:442	arg1	values					480:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values	425:485	LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort)	425:683	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	4	81	theme	characteristics	756:770	arg1	curve					772:776	the receiver operating characteristics curve	733:776	the receiver operating characteristics curve (AUC)	733:782	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
31145522	4	81	theme	characteristics	756:770	arg1	AUC					779:781	AUC	779:781	AUC	779:781	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
31145522	3	82	theme	free	456:459	arg1	PSA/tPSA					461:468	free PSA/tPSA	456:468	free PSA/tPSA (F/T PSA)	456:478	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	3	82	theme	free	456:459	arg1	PSA					475:477	F/T PSA	471:477	F/T PSA	471:477	We retrospectively measured LDN-PSA, total PSA (tPSA), and free PSA/tPSA (F/T PSA) values in 718 men who underwent a Pbx in 3 academic urology clinics in Japan and Canada (Pbx cohort) and in 174 PC patients who subsequently underwent radical prostatectomy in Australia (preop-PSA cohort).					
31145522	6	83	theme	rectal	1227:1232	arg1	examination					1234:1244	digital rectal examination	1219:1244	digital rectal examination status	1219:1251	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	2	84	theme	prostate	229:236	arg1	CSPC					246:249	CSPC	246:249	CSPC	246:249	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	2	84	theme	prostate	229:236	arg1	cancer					238:243	clinically significant prostate cancer	206:243	clinically significant prostate cancer (CSPC)	206:250	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	6	85	theme	more	1301:1304	arg1	biopsies					1306:1313	even more biopsies	1296:1313	even more biopsies	1296:1313	The decision curve analysis showed that using a risk threshold of 20% and adding LDN-PSA and LDN-PSAD to the base model (age, digital rectal examination status, tPSA, and F/T PSA) permitted avoidance of even more biopsies without missing CSPC (9.89% and 18.11%, respectively vs 2.23% [base model]).					
31145522	0	86	gly	LacdiNAc-glycosylated	29:49	arg1	antigen					69:75	the LacdiNAc-glycosylated prostate-specific antigen	25:75	the LacdiNAc-glycosylated prostate-specific antigen assay	25:81	Clinical significance of the LacdiNAc-glycosylated prostate-specific antigen assay for prostate cancer detection.					
31145522	2	87	theme	LDN-PSA	347:353	arg1	performance					270:280	the performance	266:280	the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD)	266:394	To identify clinically significant prostate cancer (CSPC) we determined the performance of LacdiNAc-glycosylated prostate-specific antigen (LDN-PSA) and LDN-PSA normalized by prostate volume (LDN-PSAD).					
31145522	4	88	theme	receiver	737:744	arg1	characteristics					756:770	the receiver operating characteristics	733:770	the receiver operating characteristics curve (AUC)	733:782	The assays were evaluated using the area under the receiver operating characteristics curve (AUC) and decision curve analyses to discriminate CSPC.					
30698702	4	0	theme	glycomic	527:534	arg1	assays					536:541	glycomic assays	527:541	glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues	527:617	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	5	1	theme	tumor	682:686	arg1	tissues					688:694	the colorectal tumor tissues	667:694	the colorectal tumor tissues	667:694	In total, aberrant N-glycans were expressed in the colorectal tumor tissues.					
30698702	8	2	theme	curve	1300:1304	arg1	value					1306:1310	the curve value	1296:1310	the curve value of 0.823	1296:1319	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	11	3	theme	colorectal	1616:1625	arg1	cancer					1627:1632	colorectal cancer	1616:1632	colorectal cancer	1616:1632	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	6	4	theme	M/Z	977:979	arg1	12282+					981:986	M/Z 12282+	977:986	M/Z 12282+ (bifucosylated N-glycan)	977:1011	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	4	theme	M/Z	977:979	arg1	N-glycan					1003:1010	bifucosylated N-glycan	989:1010	bifucosylated N-glycan	989:1010	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	7	5	theme	profile	1043:1049	arg1	data					1051:1054	the MS profile data	1036:1054	the MS profile data	1036:1054	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	11	6	theme	interesting	1582:1592	arg1	point					1594:1598	an interesting point	1579:1598	an interesting point in the study of colorectal cancer	1579:1632	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	11	6	theme	interesting	1582:1592	arg1	expression					1538:1547	the expression	1534:1547	the expression of bisecting N-glycans	1534:1570	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	6	7	theme	two-antennae	821:832	arg1	N-glycan					842:849	two-antennae complex N-glycan	821:849	two-antennae complex N-glycan	821:849	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	7	theme	two-antennae	821:832	arg1	10552+					813:818	M/Z 10552+	809:818	M/Z 10552+ (two-antennae complex N-glycan)	809:850	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	4	8	theme	ionization	484:493	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	7	9	theme	discriminant	1158:1169	arg1	analysis					1171:1178	orthogonal partial least squares discriminant analysis	1125:1178	orthogonal partial least squares discriminant analysis	1125:1178	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	4	10	from	assays	536:541	arg1	N-glycans					546:554	N-glycans	546:554	N-glycans obtained from solid CRC tissues and paired peritumoral tissues	546:617	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	5	11	theme	aberrant	630:637	arg1	N-glycans					639:647	aberrant N-glycans	630:647	aberrant N-glycans	630:647	In total, aberrant N-glycans were expressed in the colorectal tumor tissues.					
30698702	3	12	theme	colorectal	388:397	arg1	CRC					415:417	CRC	415:417	CRC	415:417	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	3	12	theme	colorectal	388:397	arg1	tissues					406:412	colorectal cancer tissues	388:412	colorectal cancer tissues (CRC)	388:418	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	2	13	theme	Tumor-associated	171:186	arg1	antigens					201:208	Tumor-associated carbohydrate antigens	171:208	Tumor-associated carbohydrate antigens	171:208	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	2	13	theme	Tumor-associated	171:186	arg1	N-glycans					221:229	N-glycans	221:229	N-glycans	221:229	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	6	14	dep	structures	727:736	arg1	13522+					790:795	13522+	790:795	13522+	790:795	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	9732+					743:747	M/Z 9732+	739:747	M/Z 9732+	739:747	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	11622+					766:771	11622+	766:771	11622+	766:771	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	11772+					774:779	11772+	774:779	11772+	774:779	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	10602+					750:755	10602+	750:755	10602+	750:755	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	10752+					758:763	10752+	758:763	10752+	758:763	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	14	dep	structures	727:736	arg1	12642+					782:787	12642+	782:787	12642+	782:787	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	10	15	theme	cell	1464:1467	arg1	carcinoma					1469:1477	esophageal squamous cell carcinoma	1444:1477	esophageal squamous cell carcinoma	1444:1477	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	9	16	theme	tumor	1397:1401	arg1	stages					1403:1408	the tumor stages	1393:1408	the tumor stages	1393:1408	Interestingly, we observed that the bisecting N-glycans decreased with the tumor stages.					
30698702	3	17	theme	aberrant	364:371	arg1	N-glycans					373:381	aberrant N-glycans	364:381	aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients	364:438	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	3	18	from	tissues	406:412	arg1	N-glycans					373:381	aberrant N-glycans	364:381	aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients	364:438	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	7	19	theme	several	1057:1063	arg1	tools					1077:1081	several statistical tools	1057:1081	several statistical tools	1057:1081	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	10	20	contain	had	1513:1515	arg1	N-glycans					1503:1511	the bisecting N-glycans	1489:1511	the bisecting N-glycans	1489:1511	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	10	20	contain	had	1513:1515	arg2	change					1520:1525	no change	1517:1525	no change	1517:1525	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	10	21	located	found	1435:1439	arg1	carcinoma					1469:1477	esophageal squamous cell carcinoma	1444:1477	esophageal squamous cell carcinoma	1444:1477	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	10	21	located	found	1435:1439	arg2	phenomenon					1416:1425	This phenomenon	1411:1425	This phenomenon	1411:1425	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	7	22	theme	squares	1150:1156	arg1	analysis					1171:1178	orthogonal partial least squares discriminant analysis	1125:1178	orthogonal partial least squares discriminant analysis	1125:1178	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	6	23	theme	three-antennae	868:881	arg1	N-glycan					891:898	three-antennae complex N-glycan	868:898	three-antennae complex N-glycan	868:898	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	23	theme	three-antennae	868:881	arg1	12792+					860:865	M/Z 12792+	856:865	M/Z 12792+ (three-antennae complex N-glycan)	856:899	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	7	24	theme	statistical	1065:1075	arg1	tools					1077:1081	several statistical tools	1057:1081	several statistical tools	1057:1081	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	1	25	theme	Aberrant	84:91	arg1	glycosylation					93:105	Aberrant glycosylation	84:105	Aberrant glycosylation	84:105	Aberrant glycosylation is strongly correlated with the development of various cancers.					
30698702	6	26	theme	M/Z	739:741	arg1	13522+					790:795	13522+	790:795	13522+	790:795	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	9732+					743:747	M/Z 9732+	739:747	M/Z 9732+	739:747	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	11622+					766:771	11622+	766:771	11622+	766:771	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	11772+					774:779	11772+	774:779	11772+	774:779	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	10602+					750:755	10602+	750:755	10602+	750:755	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	10752+					758:763	10752+	758:763	10752+	758:763	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	26	theme	M/Z	739:741	arg1	12642+					782:787	12642+	782:787	12642+	782:787	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	7	27	theme	least	1144:1148	arg1	squares					1150:1156	orthogonal partial least squares	1125:1156	orthogonal partial least squares discriminant analysis	1125:1178	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	0	28	theme	spectrometry	5:16	arg1	analysis					18:25	Mass spectrometry analysis	0:25	Mass spectrometry analysis	0:25	Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.					
30698702	10	29	theme	bisecting	1493:1501	arg1	N-glycans					1503:1511	the bisecting N-glycans	1489:1511	the bisecting N-glycans	1489:1511	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	7	30	theme	operating	1193:1201	arg1	curve					1218:1222	receiver operating characteristic curve	1184:1222	receiver operating characteristic curve	1184:1222	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	6	31	theme	complex	883:889	arg1	N-glycan					891:898	three-antennae complex N-glycan	868:898	three-antennae complex N-glycan	868:898	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	31	theme	complex	883:889	arg1	12792+					860:865	M/Z 12792+	856:865	M/Z 12792+ (three-antennae complex N-glycan)	856:899	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	32	theme	high-mannose	939:950	arg1	11162+					931:936	M/Z 11162+	927:936	M/Z 11162+ (high-mannose N-glycan)	927:960	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	32	theme	high-mannose	939:950	arg1	N-glycan					952:959	high-mannose N-glycan	939:959	high-mannose N-glycan	939:959	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	0	33	theme	Mass	0:3	arg1	analysis					18:25	Mass spectrometry analysis	0:25	Mass spectrometry analysis	0:25	Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.					
30698702	5	34	theme	colorectal	671:680	arg1	tissues					688:694	the colorectal tumor tissues	667:694	the colorectal tumor tissues	667:694	In total, aberrant N-glycans were expressed in the colorectal tumor tissues.					
30698702	4	35	theme	trap	455:458	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	3	36	theme	cancer	399:404	arg1	CRC					415:417	CRC	415:417	CRC	415:417	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	3	36	theme	cancer	399:404	arg1	tissues					406:412	colorectal cancer tissues	388:412	colorectal cancer tissues (CRC)	388:418	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	7	37	theme	MS	1040:1041	arg1	data					1051:1054	the MS profile data	1036:1054	the MS profile data	1036:1054	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	6	38	theme	bisecting	717:725	arg1	structures					727:736	seven bisecting structures	711:736	seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+)	711:796	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	11	39	theme	cancer	1627:1632	arg1	study					1607:1611	the study	1603:1611	the study of colorectal cancer	1603:1632	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	4	40	theme	peritumoral	599:609	arg1	tissues					611:617	paired peritumoral tissues	592:617	paired peritumoral tissues	592:617	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	3	41	theme	colorectal	312:321	arg1	cancer					323:328	colorectal cancer	312:328	colorectal cancer	312:328	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	4	42	theme	quadrupole-electrospray	460:482	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	3	43	theme	Chinese	423:429	arg1	patients					431:438	Chinese patients	423:438	Chinese patients	423:438	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	4	44	theme	paired	592:597	arg1	tissues					611:617	paired peritumoral tissues	592:617	paired peritumoral tissues	592:617	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	10	45	theme	squamous	1455:1462	arg1	carcinoma					1469:1477	esophageal squamous cell carcinoma	1444:1477	esophageal squamous cell carcinoma	1444:1477	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	3	46	from	N-glycans	373:381	arg1	patients					431:438	Chinese patients	423:438	Chinese patients	423:438	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	2	47	theme	tumor	267:271	arg1	surface					278:284	the tumor cell surface	263:284	the tumor cell surface	263:284	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	0	48	theme	aberrant	35:42	arg1	N-glycans					44:52	aberrant N-glycans	35:52	aberrant N-glycans	35:52	Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.					
30698702	7	49	theme	t	1117:1117	arg1	test					1119:1122	student's t test	1107:1122	student's t test	1107:1122	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	4	50	theme	mass	495:498	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	9	51	theme	bisecting	1358:1366	arg1	N-glycans					1368:1376	the bisecting N-glycans	1354:1376	the bisecting N-glycans	1354:1376	Interestingly, we observed that the bisecting N-glycans decreased with the tumor stages.					
30698702	8	52	theme	degree	1248:1253	arg1	measurement					1229:1239	The measurement	1225:1239	The measurement of the degree of bisecting N-glycans	1225:1276	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	0	53	theme	colorectal	57:66	arg1	tissues					75:81	colorectal cancer tissues	57:81	colorectal cancer tissues	57:81	Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.					
30698702	6	54	theme	M/Z	927:929	arg1	11162+					931:936	M/Z 11162+	927:936	M/Z 11162+ (high-mannose N-glycan)	927:960	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	54	theme	M/Z	927:929	arg1	N-glycan					952:959	high-mannose N-glycan	939:959	high-mannose N-glycan	939:959	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	8	55	contain	had	1278:1280	arg1	measurement					1229:1239	The measurement	1225:1239	The measurement of the degree of bisecting N-glycans	1225:1276	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	8	55	contain	had	1278:1280	arg2	area					1285:1288	an area	1282:1288	an area under the curve value of 0.823	1282:1319	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	2	56	theme	carbohydrate	188:199	arg1	antigens					201:208	Tumor-associated carbohydrate antigens	171:208	Tumor-associated carbohydrate antigens	171:208	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	2	56	theme	carbohydrate	188:199	arg1	N-glycans					221:229	N-glycans	221:229	N-glycans	221:229	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	11	57	theme	bisecting	1552:1560	arg1	N-glycans					1562:1570	bisecting N-glycans	1552:1570	bisecting N-glycans	1552:1570	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	8	58	theme	N-glycans	1268:1276	arg1	degree					1248:1253	the degree	1244:1253	the degree of bisecting N-glycans	1244:1276	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	10	59	theme	esophageal	1444:1453	arg1	carcinoma					1469:1477	esophageal squamous cell carcinoma	1444:1477	esophageal squamous cell carcinoma	1444:1477	This phenomenon was not found in esophageal squamous cell carcinoma, in which the bisecting N-glycans had no change.					
30698702	4	60	theme	CRC	576:578	arg1	tissues					580:586	solid CRC tissues	570:586	solid CRC tissues	570:586	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	3	61	from	China	341:345	arg1	high					333:336	high	333:336	high	333:336	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	3	61	from	China	341:345	arg1	incidence					299:307	the incidence	295:307	the incidence of colorectal cancer	295:328	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	8	62	theme	bisecting	1258:1266	arg1	N-glycans					1268:1276	bisecting N-glycans	1258:1276	bisecting N-glycans	1258:1276	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	4	63	theme	solid	570:574	arg1	tissues					580:586	solid CRC tissues	570:586	solid CRC tissues	570:586	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	3	64	theme	cancer	323:328	arg1	high					333:336	high	333:336	high	333:336	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	3	64	theme	cancer	323:328	arg1	incidence					299:307	the incidence	295:307	the incidence of colorectal cancer	295:328	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	6	65	theme	complex	834:840	arg1	N-glycan					842:849	two-antennae complex N-glycan	821:849	two-antennae complex N-glycan	821:849	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	65	theme	complex	834:840	arg1	10552+					813:818	M/Z 10552+	809:818	M/Z 10552+ (two-antennae complex N-glycan)	809:850	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	66	theme	M/Z	809:811	arg1	N-glycan					842:849	two-antennae complex N-glycan	821:849	two-antennae complex N-glycan	821:849	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	66	theme	M/Z	809:811	arg1	10552+					813:818	M/Z 10552+	809:818	M/Z 10552+ (two-antennae complex N-glycan)	809:850	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	8	67	theme	0.823	1315:1319	arg1	value					1306:1310	the curve value	1296:1310	the curve value of 0.823	1296:1319	The measurement of the degree of bisecting N-glycans had an area under the curve value of 0.823.					
30698702	4	68	theme	ion	451:453	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	7	69	theme	orthogonal	1125:1134	arg1	squares					1150:1156	orthogonal partial least squares	1125:1156	orthogonal partial least squares discriminant analysis	1125:1178	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	6	70	gly	bifucosylated	989:1001	arg1	12282+					981:986	M/Z 12282+	977:986	M/Z 12282+ (bifucosylated N-glycan)	977:1011	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	70	gly	bifucosylated	989:1001	arg1	N-glycan					1003:1010	bifucosylated N-glycan	989:1010	bifucosylated N-glycan	989:1010	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	71	theme	bifucosylated	989:1001	arg1	12282+					981:986	M/Z 12282+	977:986	M/Z 12282+ (bifucosylated N-glycan)	977:1011	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	71	theme	bifucosylated	989:1001	arg1	N-glycan					1003:1010	bifucosylated N-glycan	989:1010	bifucosylated N-glycan	989:1010	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	72	theme	M/Z	856:858	arg1	N-glycan					891:898	three-antennae complex N-glycan	868:898	three-antennae complex N-glycan	868:898	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	6	72	theme	M/Z	856:858	arg1	12792+					860:865	M/Z 12792+	856:865	M/Z 12792+ (three-antennae complex N-glycan)	856:899	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	4	73	theme	Linear	444:449	arg1	spectrometry					500:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	Linear ion trap quadrupole-electrospray ionization mass spectrometry	444:511	By Linear ion trap quadrupole-electrospray ionization mass spectrometry, we performed glycomic assays on N-glycans obtained from solid CRC tissues and paired peritumoral tissues.					
30698702	11	74	from	point	1594:1598	arg1	study					1607:1611	the study	1603:1611	the study of colorectal cancer	1603:1632	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	1	75	theme	various	154:160	arg1	cancers					162:168	various cancers	154:168	various cancers	154:168	Aberrant glycosylation is strongly correlated with the development of various cancers.					
30698702	7	76	theme	characteristic	1203:1216	arg1	curve					1218:1222	receiver operating characteristic curve	1184:1222	receiver operating characteristic curve	1184:1222	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30698702	3	77	from	high	333:336	arg1	China					341:345	China	341:345	China	341:345	Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients.					
30698702	0	78	theme	cancer	68:73	arg1	tissues					75:81	colorectal cancer tissues	57:81	colorectal cancer tissues	57:81	Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.					
30698702	6	79	theme	M/Z	912:914	arg1	10132+					916:921	M/Z 10132+	912:921	M/Z 10132+	912:921	Specifically, seven bisecting structures (M/Z 9732+, 10602+, 10752+, 11622+, 11772+, 12642+, 13522+) decreased, M/Z 10552+ (two-antennae complex N-glycan) and M/Z 12792+ (three-antennae complex N-glycan) decreased, M/Z 10132+ and M/Z 11162+ (high-mannose N-glycan) increased, and M/Z 12282+ (bifucosylated N-glycan) increased.					
30698702	2	80	theme	cell	273:276	arg1	surface					278:284	the tumor cell surface	263:284	the tumor cell surface	263:284	Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface.					
30698702	11	81	theme	N-glycans	1562:1570	arg1	expression					1538:1547	the expression	1534:1547	the expression of bisecting N-glycans	1534:1570	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	11	81	theme	N-glycans	1562:1570	arg1	point					1594:1598	an interesting point	1579:1598	an interesting point in the study of colorectal cancer	1579:1632	Thus, the expression of bisecting N-glycans may be an interesting point in the study of colorectal cancer.					
30698702	1	82	theme	cancers	162:168	arg1	development					139:149	the development	135:149	the development of various cancers	135:168	Aberrant glycosylation is strongly correlated with the development of various cancers.					
30698702	7	83	theme	partial	1136:1142	arg1	squares					1150:1156	orthogonal partial least squares	1125:1156	orthogonal partial least squares discriminant analysis	1125:1178	To evaluate the MS profile data, several statistical tools were applied, including student's t test, orthogonal partial least squares discriminant analysis and receiver operating characteristic curve.					
30087829	10	0	theme	structure	1106:1114	arg1	coordinates					1087:1097	The atomic coordinates	1076:1097	The atomic coordinates of the structure of DLac from Cerrena sp	1076:1138	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	7	1	from	N468	782:785	arg1	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	1	from	N468	782:785	arg1	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	5	2	theme	substrate-binding	388:404	arg1	loops					406:410	unique substrate-binding loops	381:410	unique substrate-binding loops from those in other laccases	381:439	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	5	3	theme	high	331:334	arg1	homology					336:343	high homology	331:343	high homology to monomeric laccases	331:365	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	0	4	theme	Cerrena	74:80	arg1	sp					82:83	Cerrena sp	74:83	Cerrena sp	74:83	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	0	5	from	analysis	8:15	arg1	sp					82:83	Cerrena sp	74:83	Cerrena sp	74:83	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	10	6	theme	atomic	1080:1085	arg1	coordinates					1087:1097	The atomic coordinates	1076:1097	The atomic coordinates of the structure of DLac from Cerrena sp	1076:1138	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	7	7	theme	conserved	709:717	arg1	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	8	8	theme	first	875:879	arg1	crucial					904:910	crucial	904:910	crucial	904:910	The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency.					
30087829	8	8	theme	first	875:879	arg1	N-acetylglucosamine					881:899	the first N-acetylglucosamine	871:899	the first N-acetylglucosamine	871:899	The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency.					
30087829	7	9	theme	glycosylation	719:731	arg1	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	6	10	theme	substrate-binding	509:525	arg1	loop					527:530	the short substrate-binding loop IV	499:533	the short substrate-binding loop IV	499:533	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	2	11	theme	high-efficiency	94:108	arg1	DLac					119:122	DLac	119:122	DLac	119:122	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	2	11	theme	high-efficiency	94:108	arg1	laccase					110:116	A high-efficiency laccase	92:116	A high-efficiency laccase	92:116	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	11	12	theme	Protein	1201:1207	arg1	Bank					1214:1217	the RCSB Protein Data Bank	1192:1217	the RCSB Protein Data Bank (PDB ID: 5Z1X)	1192:1232	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	11	12	theme	Protein	1201:1207	arg1	ID					1224:1225	PDB ID	1220:1225	PDB ID: 5Z1X	1220:1231	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	6	13	theme	short	503:507	arg1	loop					527:530	the short substrate-binding loop IV	499:533	the short substrate-binding loop IV	499:533	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	11	14	theme	Data	1209:1212	arg1	Bank					1214:1217	the RCSB Protein Data Bank	1192:1217	the RCSB Protein Data Bank (PDB ID: 5Z1X)	1192:1232	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	11	14	theme	Data	1209:1212	arg1	ID					1224:1225	PDB ID	1220:1225	PDB ID: 5Z1X	1220:1231	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	7	15	gly	glycosylated	632:643	arg1	laccases					652:659	highly glycosylated fungal laccases	625:659	highly glycosylated fungal laccases	625:659	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	5	16	theme	monomeric	348:356	arg1	laccases					358:365	monomeric laccases	348:365	monomeric laccases	348:365	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	9	17	theme	protein	979:985	arg1	yield					987:991	protein yield	979:991	protein yield	979:991	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	5	18	theme	crystal	235:241	arg1	structure					243:251	The crystal structure	231:251	The crystal structure of DLac	231:259	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	7	19	gly	glycosylation	719:731	arg2	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	19	gly	glycosylation	719:731	arg2	N468					782:785	N468	782:785	N468	782:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	19	gly	glycosylation	719:731	arg2	one					698:700	one	698:700	one	698:700	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	19	gly	glycosylation	719:731	arg2	N432					741:744	N432	741:744	N432	741:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	8	20	theme	substrate-binding	816:832	arg1	loops					834:838	the substrate-binding loops	812:838	the substrate-binding loops	812:838	The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency.					
30087829	10	21	theme	Cerrena	1129:1135	arg1	sp					1137:1138	Cerrena sp	1129:1138	Cerrena sp	1129:1138	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	6	22	theme	substrate	597:605	arg1	diffusion					607:615	large substrate diffusion	591:615	large substrate diffusion	591:615	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	0	23	from	studies	32:38	arg1	sp					82:83	Cerrena sp	74:83	Cerrena sp	74:83	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	9	24	theme	industrial	1042:1051	arg1	applications					1053:1064	industrial applications	1042:1064	industrial applications	1042:1064	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	11	25	theme	PDB	1220:1222	arg1	Bank					1214:1217	the RCSB Protein Data Bank	1192:1217	the RCSB Protein Data Bank (PDB ID: 5Z1X)	1192:1232	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	11	25	theme	PDB	1220:1222	arg1	ID					1224:1225	PDB ID	1220:1225	PDB ID: 5Z1X	1220:1231	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	7	26	theme	glycosylated	632:643	arg1	laccases					652:659	highly glycosylated fungal laccases	625:659	highly glycosylated fungal laccases	625:659	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	0	27	theme	Kinetic	0:6	arg1	analysis					8:15	Kinetic analysis	0:15	Kinetic analysis	0:15	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	7	28	gly	glycosylation	760:772	arg2	N468					782:785	N468	782:785	N468	782:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	28	gly	glycosylation	760:772	arg2	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	28	gly	glycosylation	760:772	arg2	N432					741:744	N432	741:744	N432	741:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	8	29	theme	catalytic	920:928	arg1	efficiency					930:939	the catalytic efficiency	916:939	the catalytic efficiency	916:939	The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency.					
30087829	7	30	contain	contains	689:696	arg2	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	30	contain	contains	689:696	arg2	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	30	contain	contains	689:696	arg1	DLac					684:687	the less-glycosylated DLac	662:687	the less-glycosylated DLac	662:687	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	0	31	theme	structural	21:30	arg1	studies					32:38	structural studies	21:38	structural studies	21:38	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	5	32	theme	overall	306:312	arg1	structure					314:322	its overall structure	302:322	its overall structure	302:322	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	5	33	from	those	417:421	arg1	loops					406:410	unique substrate-binding loops	381:410	unique substrate-binding loops from those in other laccases	381:439	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	7	34	from	N432	741:744	arg1	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	34	from	N432	741:744	arg1	site					733:736	one highly conserved glycosylation site	698:736	one highly conserved glycosylation site at N432	698:744	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	4	35	theme	kinetic	165:171	arg1	studies					173:179	The kinetic studies	161:179	The kinetic studies	161:179	The kinetic studies indicate that DLac is a diffusion-limited enzyme.					
30087829	6	36	theme	side	484:487	arg1	chain					489:493	small side chain	478:493	small side chain	478:493	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	0	37	from	sp	82:83	arg1	laccase					61:67	a high-efficiency laccase	43:67	a high-efficiency laccase from Cerrena sp	43:83	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	0	37	from	sp	82:83	arg1	analysis					8:15	Kinetic analysis	0:15	Kinetic analysis	0:15	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	0	37	from	sp	82:83	arg1	studies					32:38	structural studies	21:38	structural studies	21:38	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	11	38	dep	ID	1224:1225	arg1	5Z1X					1228:1231	5Z1X	1228:1231	PDB ID: 5Z1X	1220:1231	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	6	39	with	residues	464:471	arg1	loop					527:530	the short substrate-binding loop IV	499:533	the short substrate-binding loop IV	499:533	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	6	39	with	residues	464:471	arg1	chain					489:493	small side chain	478:493	small side chain	478:493	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	10	40	from	sp	1137:1138	arg1	coordinates					1087:1097	The atomic coordinates	1076:1097	The atomic coordinates of the structure of DLac from Cerrena sp	1076:1138	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	9	41	theme	submerged	1013:1021	arg1	method					1031:1036	the submerged culture method	1009:1036	the submerged culture method for industrial applications	1009:1064	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	6	42	theme	small	478:482	arg1	chain					489:493	small side chain	478:493	small side chain	478:493	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	11	43	theme	structural	1150:1159	arg1	factors					1161:1167	structural factors	1150:1167	structural factors	1150:1167	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	8	44	theme	protein	848:854	arg1	structure					856:864	the protein structure	844:864	the protein structure	844:864	The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency.					
30087829	9	45	theme	culture	1023:1029	arg1	method					1031:1036	the submerged culture method	1009:1036	the submerged culture method for industrial applications	1009:1064	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	5	46	theme	atomic	279:284	arg1	resolution					286:295	atomic resolution	279:295	atomic resolution	279:295	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	9	47	from	increase	967:974	arg1	yield					987:991	protein yield	979:991	protein yield	979:991	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	6	48	theme	large	591:595	arg1	diffusion					607:615	large substrate diffusion	591:615	large substrate diffusion	591:615	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	0	49	theme	high-efficiency	45:59	arg1	laccase					61:67	a high-efficiency laccase	43:67	a high-efficiency laccase from Cerrena sp	43:83	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	2	50	theme	Cerrena	143:149	arg1	sp					151:152	Cerrena sp	143:152	Cerrena sp	143:152	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	9	51	theme	fivefold	958:965	arg1	increase					967:974	a fivefold increase	956:974	a fivefold increase in protein yield	956:991	Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications.					
30087829	2	52	attach	isolated	129:136	arg2	DLac					119:122	DLac	119:122	DLac	119:122	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	2	52	attach	isolated	129:136	arg1	sp					151:152	Cerrena sp	143:152	Cerrena sp	143:152	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	2	52	attach	isolated	129:136	arg2	laccase					110:116	A high-efficiency laccase	92:116	A high-efficiency laccase	92:116	A high-efficiency laccase, DLac, was isolated from Cerrena sp.					
30087829	6	53	theme	substrate-binding	547:563	arg1	cavity					565:570	the substrate-binding cavity	543:570	the substrate-binding cavity	543:570	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	6	54	theme	substrate-binding	446:462	arg1	residues					464:471	The substrate-binding residues	442:471	The substrate-binding residues with small side chain and the short substrate-binding loop IV	442:533	The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion.					
30087829	4	55	theme	diffusion-limited	205:221	arg1	DLac					195:198	DLac	195:198	DLac	195:198	The kinetic studies indicate that DLac is a diffusion-limited enzyme.					
30087829	4	55	theme	diffusion-limited	205:221	arg1	enzyme					223:228	a diffusion-limited enzyme	203:228	a diffusion-limited enzyme	203:228	The kinetic studies indicate that DLac is a diffusion-limited enzyme.					
30087829	10	56	theme	DLac	1119:1122	arg1	structure					1106:1114	the structure	1102:1114	the structure of DLac	1102:1122	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	7	57	theme	fungal	645:650	arg1	laccases					652:659	highly glycosylated fungal laccases	625:659	highly glycosylated fungal laccases	625:659	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	11	58	theme	RCSB	1196:1199	arg1	Bank					1214:1217	the RCSB Protein Data Bank	1192:1217	the RCSB Protein Data Bank (PDB ID: 5Z1X)	1192:1232	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	11	58	theme	RCSB	1196:1199	arg1	ID					1224:1225	PDB ID	1220:1225	PDB ID: 5Z1X	1220:1231	RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X).					
30087829	7	59	theme	less-glycosylated	666:682	arg1	DLac					684:687	the less-glycosylated DLac	662:687	the less-glycosylated DLac	662:687	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	5	60	theme	other	426:430	arg1	laccases					432:439	other laccases	426:439	other laccases	426:439	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	7	61	gly	less-glycosylated	666:682	arg1	DLac					684:687	the less-glycosylated DLac	662:687	the less-glycosylated DLac	662:687	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	7	62	theme	unique	753:758	arg1	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
30087829	0	63	theme	laccase	61:67	arg1	analysis					8:15	Kinetic analysis	0:15	Kinetic analysis	0:15	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	0	63	theme	laccase	61:67	arg1	studies					32:38	structural studies	21:38	structural studies	21:38	Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp.					
30087829	5	64	theme	DLac	256:259	arg1	structure					243:251	The crystal structure	231:251	The crystal structure of DLac	231:259	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	10	65	dep	coordinates	1087:1097	arg1	DATABASE					1067:1074	DATABASE	1067:1074	DATABASE	1067:1074	DATABASE The atomic coordinates of the structure of DLac from Cerrena sp.					
30087829	5	66	theme	unique	381:386	arg1	loops					406:410	unique substrate-binding loops	381:410	unique substrate-binding loops from those in other laccases	381:439	The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases.					
30087829	7	67	theme	glycosylation	760:772	arg1	site					774:777	an unique glycosylation site	750:777	an unique glycosylation site at N468	750:785	Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468.					
31888963	6	0	theme	glycosylation	905:917	arg1	site					919:922	glycosylation site	905:922	glycosylation site	905:922	The composition of CD16a N-glycans varied by glycosylation site and donor.					
31888963	7	1	theme	IgG1	1120:1123	arg1	Fc					1125:1126	IgG1 Fc	1120:1126	IgG1 Fc	1120:1126	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	6	2	theme	CD16a	879:883	arg1	N-glycans					885:893	CD16a N-glycans	879:893	CD16a N-glycans	879:893	The composition of CD16a N-glycans varied by glycosylation site and donor.					
31888963	3	3	theme	post-translational	400:417	arg1	modification					419:430	post-translational modification	400:430	post-translational modification	400:430	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	10	4	theme	greater	1614:1620	arg1	fucosylation					1629:1640	greater branch fucosylation	1614:1640	greater branch fucosylation	1614:1640	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	11	5	theme	additional	1702:1711	arg1	analysis					1713:1720	The additional analysis	1698:1720	The additional analysis of CD32a from monocytes	1698:1744	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	3	6	theme	-linked	516:522	arg1	glycan					524:529	the asparagine(N)-linked glycan	499:529	the asparagine(N)-linked glycan	499:529	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	4	7	theme	post-translational	635:652	arg1	modifications					654:666	the post-translational modifications	631:666	the post-translational modifications of CD16a from endogenous monocytes	631:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	2	8	theme	therapeutic	282:292	arg1	mAbs					317:320	mAbs	317:320	mAbs	317:320	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	2	8	theme	therapeutic	282:292	arg1	antibodies					305:314	therapeutic monoclonal antibodies	282:314	therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity	282:359	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	1	9	theme	proper	165:170	arg1	cytotoxicity					229:240	antibody dependent cellular cytotoxicity	201:240	antibody dependent cellular cytotoxicity (ADCC)	201:247	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	9	theme	proper	165:170	arg1	functions					181:189	proper effector functions	165:189	proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP)	165:271	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	9	theme	proper	165:170	arg1	phagocytosis					253:264	phagocytosis	253:264	phagocytosis (ADCP)	253:271	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	11	10	theme	complex-type	1852:1863	arg1	N-glycans					1865:1873	a predominantly biantennary complex-type N-glycans	1824:1873	a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145)	1824:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	7	11	theme	oligomannose	1072:1083	arg1	types					1085:1089	∼20% hybrid and oligomannose types	1056:1089	∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses	1056:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	5	12	theme	N-glycans	825:833	arg1	composition					794:804	the composition	790:804	the composition of CD16a and CD32a N-glycans	790:833	Here we isolated monocytes from individual donors and characterized the composition of CD16a and CD32a N-glycans from all modified sites.					
31888963	2	13	theme	greater	344:350	arg1	affinity					352:359	greater affinity	344:359	greater affinity	344:359	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	7	14	theme	hybrid	1061:1066	arg1	types					1085:1089	∼20% hybrid and oligomannose types	1056:1089	∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses	1056:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	7	15	theme	binding	1167:1173	arg1	analyses					1175:1182	surface plasmon resonance binding analyses	1141:1182	surface plasmon resonance binding analyses	1141:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	8	16	theme	other	1245:1249	arg1	sites					1251:1255	other sites	1245:1255	other sites with >75% hybrid and oligomannose forms	1245:1295	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	10	17	theme	recent	1664:1669	arg1	analysis					1671:1678	a recent analysis	1662:1678	a recent analysis of NK cell CD16a	1662:1695	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	11	18	theme	sialic	1884:1889	arg1	acids					1891:1895	two sialic acids	1880:1895	two sialic acids at both sites (N64 and N145)	1880:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	7	19	theme	∼20	1056:1058	arg1	%					1059:1059	%	1059:1059	%	1059:1059	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	0	20	from	Analysis	23:30	arg1	Monocytes					86:94	Primary Human Monocytes	72:94	Primary Human Monocytes	72:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	10	21	theme	N-glycans	1487:1495	arg1	composition					1466:1476	The composition	1462:1476	The composition of CD16a N-glycans isolated from monocytes	1462:1519	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	0	22	theme	Human	80:84	arg1	Monocytes					86:94	Primary Human Monocytes	72:94	Primary Human Monocytes	72:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	7	23	theme	complex-type	961:972	arg1	N-glycans					986:994	primarily complex-type biantennary N-glycans	951:994	primarily complex-type biantennary N-glycans	951:994	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	2	24	theme	greater	369:375	arg1	efficacy					377:384	greater efficacy	369:384	greater efficacy	369:384	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	0	25	theme	Fc	52:53	arg1	Receptors					57:65	Antibody-binding Fc γ Receptors	35:65	Antibody-binding Fc γ Receptors from Primary Human Monocytes	35:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	10	26	attach	isolated	1497:1504	arg1	monocytes					1511:1519	monocytes	1511:1519	monocytes	1511:1519	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	10	26	attach	isolated	1497:1504	arg2	N-glycans					1487:1495	CD16a N-glycans	1481:1495	CD16a N-glycans isolated from monocytes	1481:1519	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	0	27	theme	Site-specific	0:12	arg1	Analysis					23:30	Site-specific N-glycan Analysis	0:30	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes	0:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	10	28	theme	CD16a	1691:1695	arg1	analysis					1671:1678	a recent analysis	1662:1678	a recent analysis of NK cell CD16a	1662:1695	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	9	29	theme	complex-type	1349:1360	arg1	N-glycans					1362:1370	highly processed complex-type N-glycans	1332:1370	highly processed complex-type N-glycans	1332:1370	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	10	30	theme	higher	1532:1537	arg1	proportion					1539:1548	a higher proportion	1530:1548	a higher proportion of oligomannose-type N-glycans	1530:1579	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	11	31	theme	different	1755:1763	arg1	features					1765:1772	different features	1755:1772	different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145)	1755:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	3	32	theme	binding	449:455	arg1	affinity					457:464	antibody binding affinity	440:464	antibody binding affinity	440:464	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	7	33	theme	resonance	1157:1165	arg1	analyses					1175:1182	surface plasmon resonance binding analyses	1141:1182	surface plasmon resonance binding analyses	1141:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	0	34	theme	Antibody-binding	35:50	arg1	Receptors					57:65	Antibody-binding Fc γ Receptors	35:65	Antibody-binding Fc γ Receptors from Primary Human Monocytes	35:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	7	35	theme	surface	1141:1147	arg1	resonance					1157:1165	surface plasmon resonance	1141:1165	surface plasmon resonance binding analyses	1141:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	10	36	theme	N-glycans	1571:1579	arg1	proportion					1539:1548	a higher proportion	1530:1548	a higher proportion of oligomannose-type N-glycans	1530:1579	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	1	37	theme	dependent	210:218	arg1	cytotoxicity					229:240	antibody dependent cellular cytotoxicity	201:240	antibody dependent cellular cytotoxicity (ADCC)	201:247	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	37	theme	dependent	210:218	arg1	ADCC					243:246	ADCC	243:246	ADCC	243:246	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	3	38	dep	affinity	457:464	arg1	composition					484:494	the composition	480:494	the composition of the asparagine(N)-linked glycan	480:529	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	4	39	theme	key	583:585	arg1	CD16a					549:553	CD16a	549:553	CD16a	549:553	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	4	39	theme	key	583:585	arg1	receptor					587:594	the key receptor	579:594	the key receptor for the monocyte response	579:620	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	11	40	dep	features	1765:1772	arg1	observed					1779:1786	observed	1779:1786	observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145)	1779:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	8	41	with	sites	1251:1255	arg1	forms					1291:1295	>75% hybrid and oligomannose forms	1262:1295	>75% hybrid and oligomannose forms	1262:1295	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	3	42	theme	glycan	524:529	arg1	composition					484:494	the composition	480:494	the composition of the asparagine(N)-linked glycan	480:529	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	4	43	from	modifications	654:666	arg1	monocytes					693:701	endogenous monocytes	682:701	endogenous monocytes	682:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	3	44	theme	CD16a	542:546	arg1	N162					534:537	N162	534:537	N162 of CD16a	534:546	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	6	45	theme	N-glycans	885:893	arg1	composition					864:874	The composition	860:874	The composition of CD16a N-glycans	860:893	The composition of CD16a N-glycans varied by glycosylation site and donor.					
31888963	10	46	theme	branch	1622:1627	arg1	fucosylation					1629:1640	greater branch fucosylation	1614:1640	greater branch fucosylation	1614:1640	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	11	47	theme	CD32a	1725:1729	arg1	analysis					1713:1720	The additional analysis	1698:1720	The additional analysis of CD32a from monocytes	1698:1744	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	8	48	theme	%	1265:1265	arg1	hybrid					1267:1272	>75% hybrid	1262:1272	>75% hybrid	1262:1272	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	5	49	theme	modified	844:851	arg1	sites					853:857	all modified sites	840:857	all modified sites	840:857	Here we isolated monocytes from individual donors and characterized the composition of CD16a and CD32a N-glycans from all modified sites.					
31888963	0	50	theme	γ	55:55	arg1	Receptors					57:65	Antibody-binding Fc γ Receptors	35:65	Antibody-binding Fc γ Receptors from Primary Human Monocytes	35:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	5	51	theme	individual	754:763	arg1	donors					765:770	individual donors	754:770	individual donors	754:770	Here we isolated monocytes from individual donors and characterized the composition of CD16a and CD32a N-glycans from all modified sites.					
31888963	2	52	theme	monoclonal	294:303	arg1	mAbs					317:320	mAbs	317:320	mAbs	317:320	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	2	52	theme	monoclonal	294:303	arg1	antibodies					305:314	therapeutic monoclonal antibodies	282:314	therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity	282:359	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
31888963	8	53	theme	CD16a	1189:1193	arg1	N45-glycans					1195:1205	The CD16a N45-glycans	1185:1205	The CD16a N45-glycans	1185:1205	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	8	54	theme	>75	1262:1264	arg1	%					1265:1265	%	1265:1265	%	1265:1265	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	1	55	theme	effector	172:179	arg1	cytotoxicity					229:240	antibody dependent cellular cytotoxicity	201:240	antibody dependent cellular cytotoxicity (ADCC)	201:247	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	55	theme	effector	172:179	arg1	functions					181:189	proper effector functions	165:189	proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP)	165:271	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	55	theme	effector	172:179	arg1	phagocytosis					253:264	phagocytosis	253:264	phagocytosis (ADCP)	253:271	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	0	56	from	Monocytes	86:94	arg1	Analysis					23:30	Site-specific N-glycan Analysis	0:30	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes	0:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	0	56	from	Monocytes	86:94	arg1	Receptors					57:65	Antibody-binding Fc γ Receptors	35:65	Antibody-binding Fc γ Receptors from Primary Human Monocytes	35:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	8	57	theme	hybrid	1267:1272	arg1	forms					1291:1295	>75% hybrid and oligomannose forms	1262:1295	>75% hybrid and oligomannose forms	1262:1295	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	10	58	theme	NK	1683:1684	arg1	CD16a					1691:1695	NK cell CD16a	1683:1695	NK cell CD16a	1683:1695	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	11	59	theme	N-glycans	1865:1873	arg1	presence					1812:1819	the presence	1808:1819	the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145)	1808:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	7	60	theme	%	1059:1059	arg1	hybrid					1061:1066	∼20% hybrid	1056:1066	∼20% hybrid	1056:1066	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	8	61	theme	less	1224:1227	arg1	processing					1229:1238	markedly less processing	1215:1238	markedly less processing	1215:1238	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	4	62	theme	monocyte	604:611	arg1	response					613:620	the monocyte response	600:620	the monocyte response	600:620	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	4	63	theme	endogenous	682:691	arg1	monocytes					693:701	endogenous monocytes	682:701	endogenous monocytes	682:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	7	64	theme	CD16a	1040:1044	arg1	V158					1046:1049	CD16a V158	1040:1049	CD16a V158	1040:1049	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	8	65	contain	contain	1207:1213	arg2	processing					1229:1238	markedly less processing	1215:1238	markedly less processing	1215:1238	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	8	65	contain	contain	1207:1213	arg1	N45-glycans					1195:1205	The CD16a N45-glycans	1185:1205	The CD16a N45-glycans	1185:1205	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	0	66	theme	Primary	72:78	arg1	Monocytes					86:94	Primary Human Monocytes	72:94	Primary Human Monocytes	72:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	5	67	theme	CD32a	819:823	arg1	N-glycans					825:833	CD32a N-glycans	819:833	CD32a N-glycans	819:833	Here we isolated monocytes from individual donors and characterized the composition of CD16a and CD32a N-glycans from all modified sites.					
31888963	9	68	theme	complex-type	1409:1420	arg1	N-glycans					1434:1442	complex-type biantennary N-glycans	1409:1442	complex-type biantennary N-glycans	1409:1442	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	6	69	gly	glycosylation	905:917	arg2	site					919:922	glycosylation site	905:922	glycosylation site	905:922	The composition of CD16a N-glycans varied by glycosylation site and donor.					
31888963	11	70	from	monocytes	1736:1744	arg1	analysis					1713:1720	The additional analysis	1698:1720	The additional analysis of CD32a from monocytes	1698:1744	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	11	70	from	monocytes	1736:1744	arg1	CD32a					1725:1729	CD32a	1725:1729	CD32a from monocytes	1725:1744	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	10	71	theme	CD16a	1481:1485	arg1	N-glycans					1487:1495	CD16a N-glycans	1481:1495	CD16a N-glycans isolated from monocytes	1481:1519	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	4	72	theme	CD16a	671:675	arg1	modifications					654:666	the post-translational modifications	631:666	the post-translational modifications of CD16a from endogenous monocytes	631:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	11	73	theme	biantennary	1840:1850	arg1	N-glycans					1865:1873	a predominantly biantennary complex-type N-glycans	1824:1873	a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145)	1824:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	7	74	theme	biantennary	974:984	arg1	N-glycans					986:994	primarily complex-type biantennary N-glycans	951:994	primarily complex-type biantennary N-glycans	951:994	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	11	75	dep	sites	1905:1909	arg1	N64					1912:1914	N64	1912:1914	N64	1912:1914	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	11	75	dep	sites	1905:1909	arg1	sites					1905:1909	both sites	1900:1909	both sites (N64 and N145)	1900:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	11	75	dep	sites	1905:1909	arg1	N145					1920:1923	N145	1920:1923	N145	1920:1923	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	3	76	link	-linked	516:522	arg1	glycan					524:529	the asparagine(N)-linked glycan	499:529	the asparagine(N)-linked glycan	499:529	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	9	77	theme	CD16a	1313:1317	arg1	CD16a					1313:1317	CD16a	1313:1317	CD16a	1313:1317	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	9	77	theme	CD16a	1313:1317	arg1	N38					1298:1300	N38	1298:1300	N38	1298:1300	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	11	78	from	sites	1905:1909	arg1	acids					1891:1895	two sialic acids	1880:1895	two sialic acids at both sites (N64 and N145)	1880:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	1	79	theme	cellular	220:227	arg1	cytotoxicity					229:240	antibody dependent cellular cytotoxicity	201:240	antibody dependent cellular cytotoxicity (ADCC)	201:247	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	1	79	theme	cellular	220:227	arg1	ADCC					243:246	ADCC	243:246	ADCC	243:246	FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).					
31888963	3	80	theme	antibody	440:447	arg1	affinity					457:464	antibody binding affinity	440:464	antibody binding affinity	440:464	Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a.					
31888963	0	81	theme	N-glycan	14:21	arg1	Analysis					23:30	Site-specific N-glycan Analysis	0:30	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes	0:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	11	82	with	N-glycans	1865:1873	arg1	acids					1891:1895	two sialic acids	1880:1895	two sialic acids at both sites (N64 and N145)	1880:1924	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	11	83	from	analysis	1713:1720	arg1	monocytes					1736:1744	monocytes	1736:1744	monocytes	1736:1744	The additional analysis of CD32a from monocytes revealed different features than observed for CD16a including the presence of a predominantly biantennary complex-type N-glycans with two sialic acids at both sites (N64 and N145).					
31888963	9	84	contain	contain	1324:1330	arg1	CD16a					1313:1317	CD16a	1313:1317	CD16a	1313:1317	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	9	84	contain	contain	1324:1330	arg1	N74					1306:1308	N74	1306:1308	N74	1306:1308	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	9	84	contain	contain	1324:1330	arg2	N-glycans					1362:1370	highly processed complex-type N-glycans	1332:1370	highly processed complex-type N-glycans	1332:1370	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	9	84	contain	contain	1324:1330	arg1	N38					1298:1300	N38	1298:1300	N38	1298:1300	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	10	85	theme	cell	1686:1689	arg1	CD16a					1691:1695	NK cell CD16a	1683:1695	NK cell CD16a	1683:1695	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	5	86	theme	CD16a	809:813	arg1	composition					794:804	the composition	790:804	the composition of CD16a and CD32a N-glycans	790:833	Here we isolated monocytes from individual donors and characterized the composition of CD16a and CD32a N-glycans from all modified sites.					
31888963	9	87	theme	processed	1339:1347	arg1	N-glycans					1362:1370	highly processed complex-type N-glycans	1332:1370	highly processed complex-type N-glycans	1332:1370	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	7	88	theme	plasmon	1149:1155	arg1	resonance					1157:1165	surface plasmon resonance	1141:1165	surface plasmon resonance binding analyses	1141:1182	CD16a displayed primarily complex-type biantennary N-glycans at N162, however some individuals expressed CD16a V158 with ∼20% hybrid and oligomannose types which increased affinity for IgG1 Fc according to surface plasmon resonance binding analyses.					
31888963	0	89	theme	Receptors	57:65	arg1	Analysis					23:30	Site-specific N-glycan Analysis	0:30	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes	0:94	Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.					
31888963	9	90	theme	N-acetyllactosamine	1377:1395	arg1	repeats					1397:1403	N-acetyllactosamine repeats	1377:1403	N-acetyllactosamine repeats	1377:1403	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	8	91	theme	oligomannose	1278:1289	arg1	forms					1291:1295	>75% hybrid and oligomannose forms	1262:1295	>75% hybrid and oligomannose forms	1262:1295	The CD16a N45-glycans contain markedly less processing than other sites with >75% hybrid and oligomannose forms.					
31888963	10	92	theme	oligomannose-type	1553:1569	arg1	N-glycans					1571:1579	oligomannose-type N-glycans	1553:1579	oligomannose-type N-glycans	1553:1579	The composition of CD16a N-glycans isolated from monocytes included a higher proportion of oligomannose-type N-glycans at N45 and less sialylation plus greater branch fucosylation than we observed in a recent analysis of NK cell CD16a.					
31888963	4	93	from	monocytes	693:701	arg1	CD16a					671:675	CD16a	671:675	CD16a from endogenous monocytes	671:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	4	93	from	monocytes	693:701	arg1	modifications					654:666	the post-translational modifications	631:666	the post-translational modifications of CD16a from endogenous monocytes	631:701	CD16a is widely recognized as the key receptor for the monocyte response, however the post-translational modifications of CD16a from endogenous monocytes are not described.					
31888963	9	94	theme	biantennary	1422:1432	arg1	N-glycans					1434:1442	complex-type biantennary N-glycans	1409:1442	complex-type biantennary N-glycans	1409:1442	N38 and N74 of CD16a both contain highly processed complex-type N-glycans with N-acetyllactosamine repeats and complex-type biantennary N-glycans dominate at N169.					
31888963	2	95	with	FcγRs	333:337	arg1	affinity					352:359	greater affinity	344:359	greater affinity	344:359	Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy.					
29440574	6	0	theme	cell	1087:1090	arg1	invasion					1092:1099	cell invasion	1087:1099	cell invasion	1087:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	1	theme	cells	935:939	arg1	similar					944:950	similar	944:950	similar	944:950	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	1	theme	cells	935:939	arg1	profile					890:896	The Shigella targeting profile	867:896	The Shigella targeting profile of ganglioside-loaded nonactivated T cells	867:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	4	2	theme	activated	611:619	arg1	lymphocytes					657:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes.					
29440574	2	3	theme	enteroinvasive	276:289	arg1	bacterium					291:299	the Gram-negative enteroinvasive bacterium	258:299	the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis	258:334	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	2	3	theme	enteroinvasive	276:289	arg1	Shigella					248:255	Shigella	248:255	Shigella	248:255	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	12	4	theme	CD4+	2048:2051	arg1	cells					2089:2093	nonactivated cells	2076:2093	nonactivated cells	2076:2093	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	4	theme	CD4+	2048:2051	arg1	lymphocytes					2055:2065	human-activated CD4+ T lymphocytes	2032:2065	human-activated CD4+ T lymphocytes	2032:2065	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	4	theme	CD4+	2048:2051	arg1	targets					2100:2106	targets	2100:2106	targets of Shigella	2100:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	6	5	theme	nonactivated	920:931	arg1	cells					935:939	ganglioside-loaded nonactivated T cells	901:939	ganglioside-loaded nonactivated T cells	901:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	9	6	theme	species	1681:1687	arg1	specificity					1689:1699	species specificity	1681:1699	species specificity	1681:1699	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	13	7	theme	type	2546:2549	arg1	system					2565:2570	the type III secretion system	2542:2570	the type III secretion system	2542:2570	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	0	8	theme	Glycan-Glycan	0:12	arg1	Interaction					14:24	Glycan-Glycan Interaction	0:24	Glycan-Glycan Interaction	0:24	Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes.					
29440574	13	9	theme	sugar	2367:2371	arg1	part					2379:2382	the sugar polar part	2363:2382	the sugar polar part of gangliosides	2363:2398	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	13	10	theme	secretion	2555:2563	arg1	system					2565:2570	the type III secretion system	2542:2570	the type III secretion system	2542:2570	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	2	11	theme	acute	317:321	arg1	rectocolitis					323:334	acute rectocolitis	317:334	acute rectocolitis	317:334	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	4	12	theme	human	644:648	arg1	lymphocytes					657:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes.					
29440574	1	13	theme	bacterial	110:118	arg1	glycans					129:135	bacterial and host glycans	110:135	bacterial and host glycans	110:135	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	5	14	theme	T	707:707	arg1	lymphocytes					709:719	nonactivated CD4+ T lymphocytes	689:719	nonactivated CD4+ T lymphocytes	689:719	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	2	15	theme	responsible	301:311	arg1	bacterium					291:299	the Gram-negative enteroinvasive bacterium	258:299	the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis	258:334	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	2	15	theme	responsible	301:311	arg1	Shigella					248:255	Shigella	248:255	Shigella	248:255	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	1	16	theme	bacteria	209:216	arg1	binding					187:193	the binding	183:193	the binding of pathogenic bacteria to host cells	183:230	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	14	17	theme	large	2664:2668	arg1	variety					2670:2676	a large variety	2662:2676	a large variety of glycans	2662:2687	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	14	17	theme	large	2664:2668	arg1	glycans					2681:2687	glycans	2681:2687	glycans	2681:2687	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	4	18	theme	T	655:655	arg1	lymphocytes					657:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes.					
29440574	7	19	theme	polysaccharide	1163:1176	arg1	moiety					1178:1183	the O-antigen polysaccharide moiety	1149:1183	the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen	1149:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	1	20	theme	host	221:224	arg1	cells					226:230	host cells	221:230	host cells	221:230	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	6	21	theme	activated	963:971	arg1	cells					975:979	activated T cells	963:979	activated T cells	963:979	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	8	22	theme	actin	1436:1440	arg1	polymerization					1442:1455	actin polymerization	1436:1455	actin polymerization	1436:1455	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	10	23	theme	Gram-negative	1760:1772	arg1	bacterium					1789:1797	the Gram-negative enteroinvasive bacterium	1756:1797	the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery	1756:1833	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	10	23	theme	Gram-negative	1760:1772	arg1	Shigella					1746:1753	Shigella	1746:1753	Shigella	1746:1753	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	5	24	with	loading	771:777	arg1	glycosphingolipids					820:837	sialylated glycosphingolipids	809:837	sialylated glycosphingolipids (also termed gangliosides)	809:864	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	12	25	theme	Shigella	2111:2118	arg1	cells					2089:2093	nonactivated cells	2076:2093	nonactivated cells	2076:2093	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	25	theme	Shigella	2111:2118	arg1	targets					2100:2106	targets	2100:2106	targets of Shigella	2100:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	25	theme	Shigella	2111:2118	arg1	lymphocytes					2055:2065	human-activated CD4+ T lymphocytes	2032:2065	human-activated CD4+ T lymphocytes	2032:2065	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	5	26	theme	nonactivated	689:700	arg1	lymphocytes					709:719	nonactivated CD4+ T lymphocytes	689:719	nonactivated CD4+ T lymphocytes	689:719	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	2	27	theme	Shigella	248:255	arg1	case					240:243	the case	236:243	the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis	236:334	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	9	28	theme	critical	1499:1506	arg1	role					1508:1511	the critical role	1495:1511	the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1495:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	12	29	theme	nonactivated	2076:2087	arg1	cells					2089:2093	nonactivated cells	2076:2093	nonactivated cells	2076:2093	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	29	theme	nonactivated	2076:2087	arg1	targets					2100:2106	targets	2100:2106	targets of Shigella	2100:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	29	theme	nonactivated	2076:2087	arg1	lymphocytes					2055:2065	human-activated CD4+ T lymphocytes	2032:2065	human-activated CD4+ T lymphocytes	2032:2065	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	7	30	theme	surface	1234:1240	arg1	antigen					1242:1248	the major bacterial surface antigen	1214:1248	the major bacterial surface antigen	1214:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	7	30	theme	surface	1234:1240	arg1	lipopolysaccharide					1188:1205	lipopolysaccharide	1188:1205	lipopolysaccharide (LPS)	1188:1211	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	10	31	theme	human	1857:1861	arg1	host					1863:1866	its exclusively human host	1841:1866	its exclusively human host	1841:1866	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	6	32	theme	effectors	1018:1026	arg1	injection					1005:1013	injection	1005:1013	injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion	1005:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	12	33	theme	activated	2312:2320	arg1	cells					2322:2326	activated cells	2312:2326	activated cells	2312:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	7	34	theme	major	1218:1222	arg1	antigen					1242:1248	the major bacterial surface antigen	1214:1248	the major bacterial surface antigen	1214:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	7	34	theme	major	1218:1222	arg1	lipopolysaccharide					1188:1205	lipopolysaccharide	1188:1205	lipopolysaccharide (LPS)	1188:1211	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	3	35	from	role	423:426	arg1	tropism					446:452	the tropism	442:452	the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state	442:552	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	9	36	theme	interactions	1530:1541	arg1	role					1508:1511	the critical role	1495:1511	the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1495:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	6	37	theme	injection	1005:1013	arg1	predominance					989:1000	a predominance	987:1000	a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion	987:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	9	38	theme	surface	1606:1612	arg1	Glycosylation					1579:1591	Shigella pathogenesis.IMPORTANCE Glycosylation	1546:1591	Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1546:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	10	39	theme	enteroinvasive	1774:1787	arg1	bacterium					1789:1797	the Gram-negative enteroinvasive bacterium	1756:1797	the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery	1756:1833	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	10	39	theme	enteroinvasive	1774:1787	arg1	Shigella					1746:1753	Shigella	1746:1753	Shigella	1746:1753	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	9	40	theme	Shigella	1546:1553	arg1	Glycosylation					1579:1591	Shigella pathogenesis.IMPORTANCE Glycosylation	1546:1591	Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1546:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	11	41	theme	step	1941:1944	arg1	determinants					1921:1932	the molecular determinants	1907:1932	the molecular determinants of the step of binding to host cells	1907:1969	However, the molecular determinants of the step of binding to host cells are poorly defined.					
29440574	5	42	theme	Shigella-targetable	740:758	arg1	cells					760:764	Shigella-targetable cells	740:764	Shigella-targetable cells	740:764	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	8	43	theme	T3SS	1362:1365	arg1	effectors					1367:1375	T3SS effectors	1362:1375	T3SS effectors	1362:1375	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	12	44	with	loading	2198:2204	arg1	gangliosides					2267:2278	gangliosides	2267:2278	gangliosides	2267:2278	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	44	with	loading	2198:2204	arg1	glycosphingolipids					2247:2264	sialylated glycosphingolipids	2236:2264	sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2236:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	3	45	theme	glycans	431:437	arg1	unknown					557:563	unknown	557:563	unknown	557:563	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	3	45	theme	glycans	431:437	arg1	role					423:426	the role	419:426	the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state	419:552	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	7	46	theme	lipopolysaccharide	1188:1205	arg1	moiety					1178:1183	the O-antigen polysaccharide moiety	1149:1183	the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen	1149:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	9	47	theme	host	1596:1599	arg1	surface					1606:1612	host cell surface	1596:1612	host cell surface	1596:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	13	48	theme	polysaccharide	2408:2421	arg1	moiety					2423:2428	the polysaccharide moiety	2404:2428	the polysaccharide moiety of Shigella lipopolysaccharide (LPS)	2404:2465	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	11	49	theme	host	1960:1963	arg1	cells					1965:1969	host cells	1960:1969	host cells	1960:1969	However, the molecular determinants of the step of binding to host cells are poorly defined.					
29440574	2	50	theme	Gram-negative	262:274	arg1	bacterium					291:299	the Gram-negative enteroinvasive bacterium	258:299	the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis	258:334	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	2	50	theme	Gram-negative	262:274	arg1	Shigella					248:255	Shigella	248:255	Shigella	248:255	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	14	51	theme	LPS	2581:2583	arg1	interaction					2585:2595	LPS interaction	2581:2595	LPS interaction with gangliosides	2581:2613	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	1	52	theme	host	124:127	arg1	glycans					129:135	bacterial and host glycans	110:135	bacterial and host glycans	110:135	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	12	53	located	present	2301:2307	arg1	cells					2322:2326	activated cells	2312:2326	activated cells	2312:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	53	located	present	2301:2307	arg2	gangliosides					2267:2278	gangliosides	2267:2278	gangliosides	2267:2278	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	53	located	present	2301:2307	arg2	glycosphingolipids					2247:2264	sialylated glycosphingolipids	2236:2264	sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2236:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	6	54	theme	secretion	1046:1054	arg1	system					1056:1061	the type III secretion system	1033:1061	the type III secretion system (T3SS) not resulting in cell invasion	1033:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	54	theme	secretion	1046:1054	arg1	T3SS					1064:1067	T3SS	1064:1067	T3SS	1064:1067	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	11	55	theme	binding	1949:1955	arg1	step					1941:1944	the step	1937:1944	the step of binding to host cells	1937:1969	However, the molecular determinants of the step of binding to host cells are poorly defined.					
29440574	5	56	theme	plasma	788:793	arg1	membrane					795:802	their plasma membrane	782:802	their plasma membrane	782:802	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	7	57	theme	O-antigen	1153:1161	arg1	moiety					1178:1183	the O-antigen polysaccharide moiety	1149:1183	the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen	1149:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	6	58	theme	Shigella	871:878	arg1	similar					944:950	similar	944:950	similar	944:950	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	58	theme	Shigella	871:878	arg1	profile					890:896	The Shigella targeting profile	867:896	The Shigella targeting profile of ganglioside-loaded nonactivated T cells	867:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	12	59	theme	plasma	2215:2220	arg1	membrane					2222:2229	their plasma membrane	2209:2229	their plasma membrane	2209:2229	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	10	60	theme	Cross	1732:1736	arg1	talk					1738:1741	Cross talk	1732:1741	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host	1732:1866	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	0	61	theme	Human	61:65	arg1	Lymphocytes					69:79	Human T Lymphocytes	61:79	Human T Lymphocytes	61:79	Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes.					
29440574	12	62	gly	sialylated	2236:2245	arg1	gangliosides					2267:2278	gangliosides	2267:2278	gangliosides	2267:2278	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	62	gly	sialylated	2236:2245	arg1	glycosphingolipids					2247:2264	sialylated glycosphingolipids	2236:2264	sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2236:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	13	63	theme	Shigella	2433:2440	arg1	LPS					2462:2464	LPS	2462:2464	LPS	2462:2464	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	13	63	theme	Shigella	2433:2440	arg1	lipopolysaccharide					2442:2459	Shigella lipopolysaccharide	2433:2459	Shigella lipopolysaccharide (LPS)	2433:2465	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	7	64	theme	CD4+	1286:1289	arg1	cells					1293:1297	CD4+ T cells	1286:1297	CD4+ T cells	1286:1297	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	2	65	theme	such	337:340	arg1	interactions					342:353	such interactions	337:353	such interactions	337:353	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	13	66	theme	gangliosides	2387:2398	arg1	moiety					2423:2428	the polysaccharide moiety	2404:2428	the polysaccharide moiety of Shigella lipopolysaccharide (LPS)	2404:2465	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	13	66	theme	gangliosides	2387:2398	arg1	part					2379:2382	the sugar polar part	2363:2382	the sugar polar part of gangliosides	2363:2398	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	6	67	with	similar	944:950	arg1	predominance					989:1000	a predominance	987:1000	a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion	987:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	5	68	dep	termed	845:850	arg1	also					840:843	also	840:843	also	840:843	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	11	69	theme	molecular	1911:1919	arg1	determinants					1921:1932	the molecular determinants	1907:1932	the molecular determinants of the step of binding to host cells	1907:1969	However, the molecular determinants of the step of binding to host cells are poorly defined.					
29440574	5	70	theme	membrane	795:802	arg1	loading					771:777	loading	771:777	loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides)	771:864	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	14	71	with	interaction	2585:2595	arg1	gangliosides					2602:2613	gangliosides	2602:2613	gangliosides	2602:2613	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	2	72	theme	bacterial	369:377	arg1	adherence					379:387	bacterial adherence	369:387	bacterial adherence to epithelial cells	369:407	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	12	73	theme	observation	2015:2025	arg1	advantage					1998:2006	advantage	1998:2006	advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella	1998:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	6	74	theme	T	933:933	arg1	cells					935:939	ganglioside-loaded nonactivated T cells	901:939	ganglioside-loaded nonactivated T cells	901:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	5	75	dep	glycosphingolipids	820:837	arg1	termed					845:850	termed	845:850	termed gangliosides	845:863	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	9	76	from	role	1508:1511	arg1	Glycosylation					1579:1591	Shigella pathogenesis.IMPORTANCE Glycosylation	1546:1591	Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1546:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	3	77	theme	glycosylation	489:501	arg1	pattern					503:509	pattern	503:509	pattern	503:509	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	3	78	theme	activation	537:546	arg1	state					548:552	their activation state	531:552	their activation state	531:552	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	12	79	theme	human-activated	2032:2046	arg1	cells					2089:2093	nonactivated cells	2076:2093	nonactivated cells	2076:2093	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	79	theme	human-activated	2032:2046	arg1	lymphocytes					2055:2065	human-activated CD4+ T lymphocytes	2032:2065	human-activated CD4+ T lymphocytes	2032:2065	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	79	theme	human-activated	2032:2046	arg1	targets					2100:2106	targets	2100:2106	targets of Shigella	2100:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	6	80	theme	ganglioside-loaded	901:918	arg1	cells					935:939	ganglioside-loaded nonactivated T cells	901:939	ganglioside-loaded nonactivated T cells	901:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	13	81	theme	polar	2373:2377	arg1	part					2379:2382	the sugar polar part	2363:2382	the sugar polar part of gangliosides	2363:2398	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	14	82	theme	glycans	2681:2687	arg1	variety					2670:2676	a large variety	2662:2676	a large variety of glycans	2662:2687	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	14	82	theme	glycans	2681:2687	arg1	glycans					2681:2687	glycans	2681:2687	glycans	2681:2687	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	12	83	theme	refractory	2151:2160	arg1	cells					2162:2166	the refractory cells	2147:2166	the refractory cells	2147:2166	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	5	84	theme	CD4+	702:705	arg1	lymphocytes					709:719	nonactivated CD4+ T lymphocytes	689:719	nonactivated CD4+ T lymphocytes	689:719	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	6	85	from	system	1056:1061	arg1	injection					1005:1013	injection	1005:1013	injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion	1005:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	85	from	system	1056:1061	arg1	effectors					1018:1026	effectors	1018:1026	effectors from the type III secretion system (T3SS) not resulting in cell invasion	1018:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	1	86	theme	pathogenic	198:207	arg1	bacteria					209:216	pathogenic bacteria	198:216	pathogenic bacteria	198:216	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	10	87	theme	bacillary	1815:1823	arg1	dysentery					1825:1833	bacillary dysentery	1815:1833	bacillary dysentery	1815:1833	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	9	88	theme	microorganisms	1716:1729	arg1	specificity					1689:1699	species specificity	1681:1699	species specificity	1681:1699	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	9	88	theme	microorganisms	1716:1729	arg1	tropism					1705:1711	tropism	1705:1711	tropism	1705:1711	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	12	89	attach	present	2301:2307	arg1	cells					2322:2326	activated cells	2312:2326	activated cells	2312:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	89	attach	present	2301:2307	arg2	gangliosides					2267:2278	gangliosides	2267:2278	gangliosides	2267:2278	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	89	attach	present	2301:2307	arg2	glycosphingolipids					2247:2264	sialylated glycosphingolipids	2236:2264	sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2236:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	14	90	theme	glycan-glycan	2720:2732	arg1	interactions					2734:2745	such glycan-glycan interactions	2715:2745	such glycan-glycan interactions	2715:2745	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	13	91	theme	effectors	2528:2536	arg1	injection					2515:2523	the injection	2511:2523	the injection of effectors	2511:2536	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	6	92	theme	T	973:973	arg1	cells					975:979	activated T cells	963:979	activated T cells	963:979	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	14	93	theme	immune	2847:2852	arg1	cells					2854:2858	immune cells	2847:2858	immune cells	2847:2858	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	4	94	theme	CD4+	650:653	arg1	lymphocytes					657:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes.					
29440574	6	95	theme	type	1037:1040	arg1	system					1056:1061	the type III secretion system	1033:1061	the type III secretion system (T3SS) not resulting in cell invasion	1033:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	95	theme	type	1037:1040	arg1	T3SS					1064:1067	T3SS	1064:1067	T3SS	1064:1067	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	14	96	theme	host	2825:2828	arg1	cells					2830:2834	host cells	2825:2834	host cells	2825:2834	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	14	96	theme	host	2825:2828	arg1	cells					2854:2858	immune cells	2847:2858	immune cells	2847:2858	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	14	97	theme	such	2715:2718	arg1	interactions					2734:2745	such glycan-glycan interactions	2715:2745	such glycan-glycan interactions	2715:2745	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	7	98	theme	bacterial	1224:1232	arg1	antigen					1242:1248	the major bacterial surface antigen	1214:1248	the major bacterial surface antigen	1214:1248	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	7	98	theme	bacterial	1224:1232	arg1	lipopolysaccharide					1188:1205	lipopolysaccharide	1188:1205	lipopolysaccharide (LPS)	1188:1211	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	8	99	theme	binding	1305:1311	arg1	step					1313:1316	This binding step	1300:1316	This binding step	1300:1316	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	8	99	theme	binding	1305:1311	arg1	dependent					1423:1431	dependent	1423:1431	dependent	1423:1431	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	8	99	theme	binding	1305:1311	arg1	step					1380:1383	a step	1378:1383	a step which we univocally demonstrate to be dependent on actin polymerization	1378:1455	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	9	100	theme	glycan-glycan	1516:1528	arg1	interactions					1530:1541	glycan-glycan interactions	1516:1541	glycan-glycan interactions	1516:1541	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	10	101	theme	responsible	1799:1809	arg1	bacterium					1789:1797	the Gram-negative enteroinvasive bacterium	1756:1797	the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery	1756:1833	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	10	101	theme	responsible	1799:1809	arg1	Shigella					1746:1753	Shigella	1746:1753	Shigella	1746:1753	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	13	102	theme	bacterial	2475:2483	arg1	binding					2485:2491	bacterial binding	2475:2491	bacterial binding	2475:2491	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	9	103	theme	pathogenesis.IMPORTANCE	1555:1577	arg1	Glycosylation					1579:1591	Shigella pathogenesis.IMPORTANCE Glycosylation	1546:1591	Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface	1546:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	14	104	theme	selective	2797:2805	arg1	interactions					2807:2818	selective interactions	2797:2818	selective interactions with host cells, including immune cells	2797:2858	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	9	105	dep	highlight	1485:1493	arg1	varies					1614:1619	varies	1614:1619	varies	1614:1619	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	5	106	theme	sialylated	809:818	arg1	glycosphingolipids					820:837	sialylated glycosphingolipids	809:837	sialylated glycosphingolipids (also termed gangliosides)	809:864	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	13	107	theme	lipopolysaccharide	2442:2459	arg1	moiety					2423:2428	the polysaccharide moiety	2404:2428	the polysaccharide moiety of Shigella lipopolysaccharide (LPS)	2404:2465	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	13	107	theme	lipopolysaccharide	2442:2459	arg1	part					2379:2382	the sugar polar part	2363:2382	the sugar polar part of gangliosides	2363:2398	We show that interactions between the sugar polar part of gangliosides and the polysaccharide moiety of Shigella lipopolysaccharide (LPS) promote bacterial binding, which results in the injection of effectors via the type III secretion system.					
29440574	6	108	from	injection	1005:1013	arg1	system					1056:1061	the type III secretion system	1033:1061	the type III secretion system (T3SS) not resulting in cell invasion	1033:1099	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	108	from	injection	1005:1013	arg1	T3SS					1064:1067	T3SS	1064:1067	T3SS	1064:1067	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	12	109	theme	membrane	2222:2229	arg1	loading					2198:2204	loading	2198:2204	loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2198:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	2	110	theme	epithelial	392:401	arg1	cells					403:407	epithelial cells	392:407	epithelial cells	392:407	In the case of Shigella, the Gram-negative enteroinvasive bacterium responsible for acute rectocolitis, such interactions contribute to bacterial adherence to epithelial cells.					
29440574	0	111	theme	Shigella	37:44	arg1	Tropism					46:52	Shigella Tropism	37:52	Shigella Tropism toward Human T Lymphocytes	37:79	Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes.					
29440574	9	112	theme	cell	1601:1604	arg1	surface					1606:1612	host cell surface	1596:1612	host cell surface	1596:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	14	113	theme	Shigella	2764:2771	arg1	pathogenesis					2773:2784	Shigella pathogenesis	2764:2784	Shigella pathogenesis	2764:2784	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	9	114	gly	Glycosylation	1579:1591	arg1	surface					1606:1612	host cell surface	1596:1612	host cell surface	1596:1612	Altogether, these findings highlight the critical role of glycan-glycan interactions in Shigella pathogenesis.IMPORTANCE Glycosylation of host cell surface varies with species and location in the body, thus contributing to species specificity and tropism of microorganisms.					
29440574	1	115	theme	Direct	82:87	arg1	interactions					89:100	Direct interactions	82:100	Direct interactions between bacterial and host glycans	82:135	Direct interactions between bacterial and host glycans have been recently reported to be involved in the binding of pathogenic bacteria to host cells.					
29440574	8	116	theme	effectors	1367:1375	arg1	injection					1349:1357	the subsequent injection	1334:1357	the subsequent injection of T3SS effectors	1334:1375	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	3	117	theme	immune	470:475	arg1	cells					477:481	immune cells	470:481	immune cells whose glycosylation pattern varies depending on their activation state	470:552	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	6	118	theme	targeting	880:888	arg1	similar					944:950	similar	944:950	similar	944:950	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	6	118	theme	targeting	880:888	arg1	profile					890:896	The Shigella targeting profile	867:896	The Shigella targeting profile of ganglioside-loaded nonactivated T cells	867:939	The Shigella targeting profile of ganglioside-loaded nonactivated T cells is similar to that of activated T cells, with a predominance of injection of effectors from the type III secretion system (T3SS) not resulting in cell invasion.					
29440574	8	119	dep	critical	1321:1328	arg1	step					1313:1316	This binding step	1300:1316	This binding step	1300:1316	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	8	119	dep	critical	1321:1328	arg1	dependent					1423:1431	dependent	1423:1431	dependent	1423:1431	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	8	119	dep	critical	1321:1328	arg1	step					1380:1383	a step	1378:1383	a step which we univocally demonstrate to be dependent on actin polymerization	1378:1455	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	7	120	theme	T	1291:1291	arg1	cells					1293:1297	CD4+ T cells	1286:1297	CD4+ T cells	1286:1297	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	12	121	theme	sialylated	2236:2245	arg1	gangliosides					2267:2278	gangliosides	2267:2278	gangliosides	2267:2278	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	121	theme	sialylated	2236:2245	arg1	glycosphingolipids					2247:2264	sialylated glycosphingolipids	2236:2264	sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells	2236:2326	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	5	122	gly	sialylated	809:818	arg1	glycosphingolipids					820:837	sialylated glycosphingolipids	809:837	sialylated glycosphingolipids (also termed gangliosides)	809:864	Here, we show that nonactivated CD4+ T lymphocytes can be turned into Shigella-targetable cells upon loading of their plasma membrane with sialylated glycosphingolipids (also termed gangliosides).					
29440574	3	123	theme	Shigella	457:464	arg1	tropism					446:452	the tropism	442:452	the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state	442:552	However, the role of glycans in the tropism of Shigella for immune cells whose glycosylation pattern varies depending on their activation state is unknown.					
29440574	0	124	theme	T	67:67	arg1	Lymphocytes					69:79	Human T Lymphocytes	61:79	Human T Lymphocytes	61:79	Glycan-Glycan Interaction Determines Shigella Tropism toward Human T Lymphocytes.					
29440574	12	125	theme	T	2053:2053	arg1	cells					2089:2093	nonactivated cells	2076:2093	nonactivated cells	2076:2093	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	125	theme	T	2053:2053	arg1	lymphocytes					2055:2065	human-activated CD4+ T lymphocytes	2032:2065	human-activated CD4+ T lymphocytes	2032:2065	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	12	125	theme	T	2053:2053	arg1	targets					2100:2106	targets	2100:2106	targets of Shigella	2100:2118	Taking advantage of the observation that human-activated CD4+ T lymphocytes, but not nonactivated cells, are targets of Shigella, we succeeded in rendering the refractory cells susceptible to targeting upon loading of their plasma membrane with sialylated glycosphingolipids (gangliosides) that are abundantly present on activated cells.					
29440574	10	126	with	talk	1738:1741	arg1	host					1863:1866	its exclusively human host	1841:1866	its exclusively human host	1841:1866	Cross talk by Shigella, the Gram-negative enteroinvasive bacterium responsible for bacillary dysentery, with its exclusively human host has been extensively studied.					
29440574	7	127	theme	Shigella	1266:1273	arg1	binding					1275:1281	Shigella binding	1266:1281	Shigella binding to CD4+ T cells	1266:1297	We demonstrate that gangliosides interact with the O-antigen polysaccharide moiety of lipopolysaccharide (LPS), the major bacterial surface antigen, thus promoting Shigella binding to CD4+ T cells.					
29440574	8	128	theme	subsequent	1338:1347	arg1	injection					1349:1357	the subsequent injection	1334:1357	the subsequent injection of T3SS effectors	1334:1375	This binding step is critical for the subsequent injection of T3SS effectors, a step which we univocally demonstrate to be dependent on actin polymerization.					
29440574	14	129	with	interactions	2807:2818	arg1	cells					2830:2834	host cells	2825:2834	host cells	2825:2834	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	14	129	with	interactions	2807:2818	arg1	cells					2854:2858	immune cells	2847:2858	immune cells	2847:2858	Whereas LPS interaction with gangliosides was proposed long ago and recently extended to a large variety of glycans, our findings reveal that such glycan-glycan interactions are critical for Shigella pathogenesis by driving selective interactions with host cells, including immune cells.					
29440574	4	130	theme	nonactivated	630:641	arg1	lymphocytes					657:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	activated, but not nonactivated, human CD4+ T lymphocytes	611:667	We previously reported that Shigella targets activated, but not nonactivated, human CD4+ T lymphocytes.					
30033944	5	0	theme	branched	691:698	arg1	N-glycans					711:719	branched sialylated N-glycans	691:719	branched sialylated N-glycans	691:719	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	4	1	theme	mouse	598:602	arg1	brain					604:608	the mouse brain	594:608	the mouse brain	594:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	11	2	theme	glycan	1452:1457	arg1	clusters					1459:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	6	3	theme	N-glycans	860:868	arg1	localization					824:835	localization	824:835	localization	824:835	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	6	3	theme	N-glycans	860:868	arg1	proteins					789:796	the carrier proteins	777:796	the carrier proteins	777:796	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	6	3	theme	N-glycans	860:868	arg1	partners					811:818	interacting partners	799:818	interacting partners	799:818	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	11	4	theme	immunoglobulin-like	1504:1522	arg1	molecule					1587:1594	a microglia-specific molecule	1566:1594	a microglia-specific molecule	1566:1594	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	11	4	theme	immunoglobulin-like	1504:1522	arg1	lectin					1524:1529	sialic acid-binding immunoglobulin-like lectin	1484:1529	sialic acid-binding immunoglobulin-like lectin H (Siglec-H)	1484:1542	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	6	5	theme	branched	840:847	arg1	N-glycans					860:868	branched sialylated N-glycans	840:868	branched sialylated N-glycans	840:868	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	11	6	theme	sialic	1484:1489	arg1	molecule					1587:1594	a microglia-specific molecule	1566:1594	a microglia-specific molecule	1566:1594	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	11	6	theme	sialic	1484:1489	arg1	lectin					1524:1529	sialic acid-binding immunoglobulin-like lectin	1484:1529	sialic acid-binding immunoglobulin-like lectin H (Siglec-H)	1484:1542	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	9	7	theme	2F	1236:1237	arg1	carriers					1239:1246	the candidate A2G'2F carriers	1218:1246	the candidate A2G'2F carriers	1218:1246	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	0	8	theme	Branched	0:7	arg1	N-glycans					20:28	Branched Sialylated N-glycans	0:28	Branched Sialylated N-glycans	0:28	Branched Sialylated N-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H.					
30033944	1	9	theme	system	185:190	arg1	function					192:199	nervous system function	177:199	nervous system function	177:199	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	5	10	theme	sialylated	700:709	arg1	N-glycans					711:719	branched sialylated N-glycans	691:719	branched sialylated N-glycans	691:719	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	9	11	theme	calreticulin	1258:1269	arg1	expression					1271:1280	calreticulin expression	1258:1280	calreticulin expression	1258:1280	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	11	12	theme	branched	1432:1439	arg1	clusters					1459:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	7	13	theme	brain	1113:1117	arg1	proteins					1119:1126	subcellular fractionated mouse brain proteins	1082:1126	subcellular fractionated mouse brain proteins	1082:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	2	14	theme	proteins	246:253	arg1	Identification					202:215	Identification	202:215	Identification of the localization, carrier proteins and interacting partners of N-glycans	202:291	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	7	15	theme	two-dimensional	1042:1056	arg1	gels					1074:1077	the two-dimensional electrophoresis gels	1038:1077	the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins	1038:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	12	16	theme	A2G	1660:1662	arg1	2F					1664:1665	branched sialylated A2G'2F	1640:1665	branched sialylated A2G'2F in synaptosomes	1640:1681	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	7	17	theme	fractionated	1094:1105	arg1	proteins					1119:1126	subcellular fractionated mouse brain proteins	1082:1126	subcellular fractionated mouse brain proteins	1082:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	12	18	gly	sialylated	1649:1658	arg1	2F					1664:1665	branched sialylated A2G'2F	1640:1665	branched sialylated A2G'2F in synaptosomes	1640:1681	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	9	19	theme	candidate	1222:1230	arg1	carriers					1239:1246	the candidate A2G'2F carriers	1218:1246	the candidate A2G'2F carriers	1218:1246	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	3	20	theme	present	356:362	arg1	study					364:368	The present study	352:368	The present study	352:368	The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-).					
30033944	5	21	gly	sialylated	700:709	arg1	N-glycans					711:719	branched sialylated N-glycans	691:719	branched sialylated N-glycans	691:719	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	9	22	theme	synaptosomal	1320:1331	arg1	fractions					1333:1341	synaptosomal fractions	1320:1341	synaptosomal fractions	1320:1341	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	2	23	theme	interacting	259:269	arg1	partners					271:278	interacting partners	259:278	interacting partners	259:278	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	1	24	theme	brain	155:159	arg1	development					161:171	normal brain development	148:171	normal brain development	148:171	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	12	25	theme	branched	1640:1647	arg1	2F					1664:1665	branched sialylated A2G'2F	1640:1665	branched sialylated A2G'2F in synaptosomes	1640:1681	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	5	26	from	expression	615:624	arg1	brain					639:643	the mouse brain	629:643	the mouse brain	629:643	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	4	27	theme	branched	544:551	arg1	2F					568:569	branched sialylated A2G'2F	544:569	branched sialylated A2G'2F	544:569	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	27	theme	branched	544:551	arg1	N-glycan					582:589	a major N-glycan	574:589	a major N-glycan in the mouse brain	574:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	9	28	theme	A2G	1232:1234	arg1	carriers					1239:1246	the candidate A2G'2F carriers	1218:1246	the candidate A2G'2F carriers	1218:1246	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	12	29	theme	spines	1728:1733	arg1	interaction					1703:1713	the interaction	1699:1713	the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H	1699:1836	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	4	30	theme	sialic	508:513	arg1	feature					531:537	a branched sialic acid structural feature	497:537	a branched sialic acid structural feature	497:537	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	1	31	gly	N-glycosylation	103:117	arg1	brain					155:159	normal brain development	148:171	normal brain development	148:171	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	1	31	gly	N-glycosylation	103:117	arg1	proteins					122:129	proteins	122:129	proteins	122:129	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	1	31	gly	N-glycosylation	103:117	arg1	system					185:190	nervous system function	177:199	nervous system function	177:199	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	12	32	theme	microglia.Key	1740:1752	arg1	words					1754:1758	microglia.Key words	1740:1758	microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H	1740:1836	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	4	33	theme	structural	520:529	arg1	feature					531:537	a branched sialic acid structural feature	497:537	a branched sialic acid structural feature	497:537	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	34	from	N-glycan	582:589	arg1	brain					604:608	the mouse brain	594:608	the mouse brain	594:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	1	35	theme	Proper	96:101	arg1	N-glycosylation					103:117	Proper N-glycosylation	96:117	Proper N-glycosylation of proteins	96:129	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	10	36	theme	cultured	1393:1400	arg1	neurons					1411:1417	cultured cortical neurons	1393:1417	cultured cortical neurons	1393:1417	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	0	37	theme	Brain	49:53	arg1	Synaptosomes					55:66	Brain Synaptosomes	49:66	Brain Synaptosomes	49:66	Branched Sialylated N-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H.					
30033944	12	38	from	2F	1664:1665	arg1	synaptosomes					1670:1681	synaptosomes	1670:1681	synaptosomes	1670:1681	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	8	39	theme	mouse	1155:1159	arg1	synaptosomes					1167:1178	mouse brain synaptosomes	1155:1178	mouse brain synaptosomes	1155:1178	A2G'2F was accumulated in mouse brain synaptosomes.					
30033944	12	40	with	interaction	1703:1713	arg1	words					1754:1758	microglia.Key words	1740:1758	microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H	1740:1836	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	11	41	theme	sialylated	1441:1450	arg1	clusters					1459:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	10	42	theme	neurons	1411:1417	arg1	spines					1383:1388	dendritic spines	1373:1388	dendritic spines of cultured cortical neurons	1373:1417	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	11	43	theme	acid-binding	1491:1502	arg1	molecule					1587:1594	a microglia-specific molecule	1566:1594	a microglia-specific molecule	1566:1594	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	11	43	theme	acid-binding	1491:1502	arg1	lectin					1524:1529	sialic acid-binding immunoglobulin-like lectin	1484:1529	sialic acid-binding immunoglobulin-like lectin H (Siglec-H)	1484:1542	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	6	44	theme	sialylated	849:858	arg1	N-glycans					860:868	branched sialylated N-glycans	840:868	branched sialylated N-glycans	840:868	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	5	45	theme	mouse	633:637	arg1	brain					639:643	the mouse brain	629:643	the mouse brain	629:643	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	12	46	dep	words	1754:1758	arg1	N-glycan					1761:1768	N-glycan	1761:1768	N-glycan	1761:1768	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	46	dep	words	1754:1758	arg1	spine					1822:1826	dendritic spine	1812:1826	dendritic spine	1812:1826	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	46	dep	words	1754:1758	arg1	fractionation					1783:1795	subcellular fractionation	1771:1795	subcellular fractionation	1771:1795	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	46	dep	words	1754:1758	arg1	Siglec-H					1829:1836	Siglec-H	1829:1836	Siglec-H	1829:1836	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	46	dep	words	1754:1758	arg1	calreticulin					1798:1809	calreticulin	1798:1809	calreticulin	1798:1809	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	1	47	theme	nervous	177:183	arg1	function					192:199	nervous system function	177:199	nervous system function	177:199	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	12	48	theme	dendritic	1812:1820	arg1	spine					1822:1826	dendritic spine	1812:1826	dendritic spine	1812:1826	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	48	theme	dendritic	1812:1820	arg1	N-glycan					1761:1768	N-glycan	1761:1768	N-glycan	1761:1768	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	9	49	theme	carriers	1239:1246	arg1	carriers					1239:1246	the candidate A2G'2F carriers	1218:1246	the candidate A2G'2F carriers	1218:1246	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	9	49	theme	carriers	1239:1246	arg1	one					1211:1213	one	1211:1213	one	1211:1213	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	2	50	theme	glycoproteins	337:349	arg1	roles					328:332	the roles	324:332	the roles of glycoproteins	324:349	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	4	51	gly	sialylated	553:562	arg1	2F					568:569	branched sialylated A2G'2F	544:569	branched sialylated A2G'2F	544:569	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	51	gly	sialylated	553:562	arg1	N-glycan					582:589	a major N-glycan	574:589	a major N-glycan in the mouse brain	574:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	5	52	theme	essential	726:734	arg1	roles					736:740	essential roles	726:740	essential roles	726:740	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	6	53	theme	interacting	799:809	arg1	partners					811:818	interacting partners	799:818	interacting partners	799:818	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	11	54	theme	Synthesized	1420:1430	arg1	clusters					1459:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	7	55	theme	mouse	1107:1111	arg1	proteins					1119:1126	subcellular fractionated mouse brain proteins	1082:1126	subcellular fractionated mouse brain proteins	1082:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	6	56	gly	sialylated	849:858	arg1	N-glycans					860:868	branched sialylated N-glycans	840:868	branched sialylated N-glycans	840:868	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	4	57	theme	branched	499:506	arg1	feature					531:537	a branched sialic acid structural feature	497:537	a branched sialic acid structural feature	497:537	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	11	58	dep	lectin	1524:1529	arg1	H					1531:1531	H (Siglec-H)	1531:1542	sialic acid-binding immunoglobulin-like lectin H (Siglec-H)	1484:1542	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	7	59	theme	subcellular	1082:1092	arg1	proteins					1119:1126	subcellular fractionated mouse brain proteins	1082:1126	subcellular fractionated mouse brain proteins	1082:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	2	60	theme	carrier	238:244	arg1	proteins					246:253	carrier proteins	238:253	carrier proteins	238:253	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	7	61	theme	electrophoresis	1058:1072	arg1	gels					1074:1077	the two-dimensional electrophoresis gels	1038:1077	the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins	1038:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	1	62	theme	proteins	122:129	arg1	N-glycosylation					103:117	Proper N-glycosylation	96:117	Proper N-glycosylation of proteins	96:129	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	10	63	located	observed	1361:1368	arg1	spines					1383:1388	dendritic spines	1373:1388	dendritic spines of cultured cortical neurons	1373:1417	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	10	63	located	observed	1361:1368	arg2	Calreticulin					1344:1355	Calreticulin	1344:1355	Calreticulin	1344:1355	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	7	64	theme	proteins	1119:1126	arg1	gels					1074:1077	the two-dimensional electrophoresis gels	1038:1077	the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins	1038:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	9	65	theme	endoplasmic	1294:1304	arg1	reticulum					1306:1314	the endoplasmic reticulum	1290:1314	the endoplasmic reticulum	1290:1314	We identified calreticulin as one of the candidate A2G'2F carriers and found calreticulin expression in both the endoplasmic reticulum and synaptosomal fractions.					
30033944	2	66	theme	N-glycans	283:291	arg1	proteins					246:253	carrier proteins	238:253	carrier proteins	238:253	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	2	66	theme	N-glycans	283:291	arg1	partners					271:278	interacting partners	259:278	interacting partners	259:278	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	2	66	theme	N-glycans	283:291	arg1	localization					224:235	the localization	220:235	the localization	220:235	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	12	67	theme	subcellular	1771:1781	arg1	N-glycan					1761:1768	N-glycan	1761:1768	N-glycan	1761:1768	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	12	67	theme	subcellular	1771:1781	arg1	fractionation					1783:1795	subcellular fractionation	1771:1795	subcellular fractionation	1771:1795	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	2	68	theme	partners	271:278	arg1	Identification					202:215	Identification	202:215	Identification of the localization, carrier proteins and interacting partners of N-glycans	202:291	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	1	69	theme	normal	148:153	arg1	development					161:171	normal brain development	148:171	normal brain development	148:171	Proper N-glycosylation of proteins is important for normal brain development and nervous system function.					
30033944	12	70	theme	sialylated	1649:1658	arg1	2F					1664:1665	branched sialylated A2G'2F	1640:1665	branched sialylated A2G'2F in synaptosomes	1640:1681	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	7	71	theme	small	1009:1013	arg1	fragments					1015:1023	small fragments	1009:1023	small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins	1009:1126	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	4	72	theme	sialylated	553:562	arg1	2F					568:569	branched sialylated A2G'2F	544:569	branched sialylated A2G'2F	544:569	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	72	theme	sialylated	553:562	arg1	N-glycan					582:589	a major N-glycan	574:589	a major N-glycan in the mouse brain	574:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	3	73	dep	2F	396:397	arg1	Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3					400:448	Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3	400:448	Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3	400:448	The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-).					
30033944	11	74	theme	microglia-specific	1568:1585	arg1	molecule					1587:1594	a microglia-specific molecule	1566:1594	a microglia-specific molecule	1566:1594	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	11	74	theme	microglia-specific	1568:1585	arg1	lectin					1524:1529	sialic acid-binding immunoglobulin-like lectin	1484:1529	sialic acid-binding immunoglobulin-like lectin H (Siglec-H)	1484:1542	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	4	75	theme	acid	515:518	arg1	feature					531:537	a branched sialic acid structural feature	497:537	a branched sialic acid structural feature	497:537	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	3	76	dep	Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3	400:448	arg1	Manβ1-4GlcNAcβ1-4[Fucα1-6					450:474	Manβ1-4GlcNAcβ1-4[Fucα1-6	450:474	Manβ1-4GlcNAcβ1-4[Fucα1-6	450:474	The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-).					
30033944	3	76	dep	Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3	400:448	arg1	GlcNAc-					476:482	GlcNAc-	476:482	GlcNAc-	476:482	The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-).					
30033944	12	77	theme	dendritic	1718:1726	arg1	spines					1728:1733	dendritic spines	1718:1733	dendritic spines	1718:1733	Taken together, these results suggest that branched sialylated A2G'2F in synaptosomes plays a role in the interaction of dendritic spines with microglia.Key words: N-glycan, subcellular fractionation, calreticulin, dendritic spine, Siglec-H.					
30033944	0	78	theme	Sialylated	9:18	arg1	N-glycans					20:28	Branched Sialylated N-glycans	0:28	Branched Sialylated N-glycans	0:28	Branched Sialylated N-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H.					
30033944	4	79	theme	major	576:580	arg1	2F					568:569	branched sialylated A2G'2F	544:569	branched sialylated A2G'2F	544:569	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	79	theme	major	576:580	arg1	N-glycan					582:589	a major N-glycan	574:589	a major N-glycan in the mouse brain	574:608	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	7	80	from	samples	955:961	arg1	N-glycans					934:942	N-glycans	934:942	N-glycans from trace samples	934:961	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	10	81	theme	dendritic	1373:1381	arg1	spines					1383:1388	dendritic spines	1373:1388	dendritic spines of cultured cortical neurons	1373:1417	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	11	82	gly	sialylated	1441:1450	arg1	clusters					1459:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters	1420:1466	Synthesized branched sialylated glycan clusters interacted with sialic acid-binding immunoglobulin-like lectin H (Siglec-H), which is known to be a microglia-specific molecule.					
30033944	10	83	theme	cortical	1402:1409	arg1	neurons					1411:1417	cultured cortical neurons	1393:1417	cultured cortical neurons	1393:1417	Calreticulin was observed in dendritic spines of cultured cortical neurons.					
30033944	4	84	contain	has	493:495	arg2	feature					531:537	a branched sialic acid structural feature	497:537	a branched sialic acid structural feature	497:537	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	4	84	contain	has	493:495	arg1	2F					490:491	A2G'2F	486:491	A2G'2F	486:491	A2G'2F has a branched sialic acid structural feature, and branched sialylated A2G'2F is a major N-glycan in the mouse brain.					
30033944	5	85	theme	brain	749:753	arg1	development					755:765	brain development	749:765	brain development	749:765	Its expression in the mouse brain increases during development, suggesting that branched sialylated N-glycans play essential roles during brain development.					
30033944	2	86	theme	localization	224:235	arg1	Identification					202:215	Identification	202:215	Identification of the localization, carrier proteins and interacting partners of N-glycans	202:291	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30033944	6	87	theme	carrier	781:787	arg1	proteins					789:796	the carrier proteins	777:796	the carrier proteins	777:796	However, the carrier proteins, interacting partners and localization of branched sialylated N-glycans remain unknown.					
30033944	8	88	theme	brain	1161:1165	arg1	synaptosomes					1167:1178	mouse brain synaptosomes	1155:1178	mouse brain synaptosomes	1155:1178	A2G'2F was accumulated in mouse brain synaptosomes.					
30033944	7	89	theme	trace	949:953	arg1	samples					955:961	trace samples	949:961	trace samples	949:961	We previously improved our method for analyzing N-glycans from trace samples, and here we succeeded in detecting A2G'2F in small fragments excised from the two-dimensional electrophoresis gels of subcellular fractionated mouse brain proteins.					
30033944	2	90	gly	glycoproteins	337:349	arg1	glycoproteins					337:349	glycoproteins	337:349	glycoproteins	337:349	Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins.					
30335377	0	0	theme	Granular	103:110	arg1	Sludge					112:117	Full-Scale Anammox Granular Sludge	84:117	Full-Scale Anammox Granular Sludge	84:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	5	1	contain	carrying	851:858	arg1	glycoprotein					837:848	a high abundant glycoprotein	821:848	a high abundant glycoprotein	821:848	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	5	1	contain	carrying	851:858	arg2	structure					885:893	a heterogeneous O-glycan structure	860:893	a heterogeneous O-glycan structure	860:893	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	1	2	theme	established	165:175	arg1	OXidation					139:147	ANaerobic AMMonium OXidation	120:147	ANaerobic AMMonium OXidation (anammox)	120:157	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	1	2	theme	established	165:175	arg1	process					177:183	an established process	162:183	an established process for efficient nitrogen removal from wastewater	162:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	0	3	theme	Anammox	95:101	arg1	Sludge					112:117	Full-Scale Anammox Granular Sludge	84:117	Full-Scale Anammox Granular Sludge	84:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	7	4	theme	large	1100:1104	arg1	fraction					1106:1113	a large fraction	1098:1113	a large fraction of the EPS	1098:1124	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	5	5	gly	glycoprotein	837:848	arg1	glycoprotein					837:848	a high abundant glycoprotein	821:848	a high abundant glycoprotein	821:848	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	2	6	theme	anammox	355:361	arg1	granules					363:370	anammox granules	355:370	anammox granules	355:370	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	1	7	theme	stable	269:274	arg1	biofilms					276:283	stable biofilms	269:283	stable biofilms	269:283	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	9	8	theme	different	1510:1518	arg1	glycoconjugates					1520:1534	different glycoconjugates	1510:1534	different glycoconjugates	1510:1534	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	6	9	gly	glycosylation	926:938	arg2	identical					959:967	identical	959:967	identical	959:967	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	6	9	gly	glycosylation	926:938	arg2	motif					949:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	9	10	theme	future	1539:1544	arg1	studies					1550:1556	future EPS studies	1539:1556	future EPS studies	1539:1556	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	8	11	theme	organisms	1353:1361	arg1	matrix					1329:1334	the extracellular matrix	1311:1334	the extracellular matrix of multicellular organisms	1311:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	9	12	theme	distinct	1412:1419	arg1	roles					1421:1425	distinct roles	1412:1425	distinct roles	1412:1425	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	0	13	theme	Sludge	112:117	arg1	Substances					70:79	Extracellular Polymeric Substances	46:79	Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge	46:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	3	14	theme	proteins	527:534	arg1	nature					513:518	the nature	509:518	the nature of the proteins	509:534	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	2	15	theme	granules	363:370	arg1	stability					342:350	stability	342:350	stability	342:350	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	2	15	theme	granules	363:370	arg1	formation					316:324	formation	316:324	formation	316:324	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	2	15	theme	granules	363:370	arg1	structure					327:335	structure	327:335	structure	327:335	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	9	16	theme	granular	1444:1451	arg1	sludge					1453:1458	granular sludge	1444:1458	granular sludge	1444:1458	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	4	17	theme	granular	659:666	arg1	sludge					668:673	full-scale anammox granular sludge	640:673	full-scale anammox granular sludge	640:673	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	8	18	theme	granules	1258:1265	arg1	structuring					1235:1245	the structuring	1231:1245	the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms	1231:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	4	19	theme	denaturing	730:739	arg1	electrophoresis					760:774	denaturing polyacrylamide gel electrophoresis	730:774	denaturing polyacrylamide gel electrophoresis	730:774	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	7	20	gly	glycosylated	1071:1082	arg1	protein					1084:1090	The heavily glycosylated protein	1059:1090	The heavily glycosylated protein	1059:1090	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	7	20	gly	glycosylated	1071:1082	arg1	located					1139:1145	located	1139:1145	located	1139:1145	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	5	21	theme	high	823:826	arg1	glycoprotein					837:848	a high abundant glycoprotein	821:848	a high abundant glycoprotein	821:848	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	7	22	theme	EPS	1122:1124	arg1	fraction					1106:1113	a large fraction	1098:1113	a large fraction of the EPS	1098:1124	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	5	23	theme	O-glycan	876:883	arg1	structure					885:893	a heterogeneous O-glycan structure	860:893	a heterogeneous O-glycan structure	860:893	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	8	24	theme	extracellular	1315:1327	arg1	matrix					1329:1334	the extracellular matrix	1311:1334	the extracellular matrix of multicellular organisms	1311:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	5	25	theme	heterogeneous	862:874	arg1	structure					885:893	a heterogeneous O-glycan structure	860:893	a heterogeneous O-glycan structure	860:893	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	1	26	theme	efficient	189:197	arg1	removal					208:214	efficient nitrogen removal	189:214	efficient nitrogen removal from wastewater	189:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	9	27	theme	glycoconjugates	1520:1534	arg1	characterization					1490:1505	more in-depth characterization	1476:1505	more in-depth characterization of different glycoconjugates	1476:1534	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	4	28	theme	Brocadia	702:709	arg1	"					710:710	" Candidatus Brocadia"	689:710	" Candidatus Brocadia"	689:710	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	9	29	contain	have	1407:1410	arg2	roles					1421:1425	distinct roles	1412:1425	distinct roles	1412:1425	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	9	29	contain	have	1407:1410	arg1	glycoconjugates					1387:1401	different glycoconjugates	1377:1401	different glycoconjugates	1377:1401	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	1	30	theme	nitrogen	199:206	arg1	removal					208:214	efficient nitrogen removal	189:214	efficient nitrogen removal from wastewater	189:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	2	31	theme	substances	449:458	arg1	composition					406:416	the composition	402:416	the composition of the extracellular polymeric substances (EPS)	402:464	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	7	32	theme	glycosylated	1071:1082	arg1	protein					1084:1090	The heavily glycosylated protein	1059:1090	The heavily glycosylated protein	1059:1090	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	7	32	theme	glycosylated	1071:1082	arg1	located					1139:1145	located	1139:1145	located	1139:1145	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	8	33	from	importance	1286:1295	arg1	matrix					1329:1334	the extracellular matrix	1311:1334	the extracellular matrix of multicellular organisms	1311:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	4	34	theme	Candidatus	691:700	arg1	"					710:710	" Candidatus Brocadia"	689:710	" Candidatus Brocadia"	689:710	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	6	35	theme	stuttgartiensis	1041:1055	arg1	protein					1008:1014	the surface layer protein	990:1014	the surface layer protein of " Candidatus Kuenenia stuttgartiensis"	990:1056	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	2	36	theme	polymeric	439:447	arg1	substances					449:458	the extracellular polymeric substances	421:458	the extracellular polymeric substances (EPS)	421:464	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	2	36	theme	polymeric	439:447	arg1	EPS					461:463	EPS	461:463	EPS	461:463	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	5	37	theme	further	780:786	arg1	analysis					788:795	further analysis	780:795	further analysis with mass spectrometry	780:818	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	1	38	theme	ANaerobic	120:128	arg1	anammox					150:156	anammox	150:156	anammox	150:156	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	1	38	theme	ANaerobic	120:128	arg1	OXidation					139:147	ANaerobic AMMonium OXidation	120:147	ANaerobic AMMonium OXidation (anammox)	120:157	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	1	38	theme	ANaerobic	120:128	arg1	process					177:183	an established process	162:183	an established process for efficient nitrogen removal from wastewater	162:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	5	39	theme	mass	802:805	arg1	spectrometry					807:818	mass spectrometry	802:818	mass spectrometry	802:818	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	2	40	theme	extracellular	425:437	arg1	substances					449:458	the extracellular polymeric substances	421:458	the extracellular polymeric substances (EPS)	421:464	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	2	40	theme	extracellular	425:437	arg1	EPS					461:463	EPS	461:463	EPS	461:463	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	1	41	theme	AMMonium	130:137	arg1	anammox					150:156	anammox	150:156	anammox	150:156	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	1	41	theme	AMMonium	130:137	arg1	OXidation					139:147	ANaerobic AMMonium OXidation	120:147	ANaerobic AMMonium OXidation (anammox)	120:157	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	1	41	theme	AMMonium	130:137	arg1	process					177:183	an established process	162:183	an established process for efficient nitrogen removal from wastewater	162:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	6	42	theme	Candidatus	1021:1030	arg1	stuttgartiensis					1041:1055	" Candidatus Kuenenia stuttgartiensis"	1019:1056	" Candidatus Kuenenia stuttgartiensis"	1019:1056	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	0	43	theme	Extracellular	46:58	arg1	Substances					70:79	Extracellular Polymeric Substances	46:79	Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge	46:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	6	44	theme	sequence	940:947	arg1	motif					949:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	6	44	theme	sequence	940:947	arg1	identical					959:967	identical	959:967	identical	959:967	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	8	45	from	role	1206:1209	arg1	structuring					1235:1245	the structuring	1231:1245	the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms	1231:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	7	46	theme	lectin	1150:1155	arg1	staining					1157:1164	lectin staining	1150:1164	lectin staining	1150:1164	The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining.					
30335377	8	47	theme	glycoproteins	1214:1226	arg1	role					1206:1209	an important role	1193:1209	an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms	1193:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	8	48	theme	anammox	1250:1256	arg1	granules					1258:1265	anammox granules	1250:1265	anammox granules	1250:1265	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	6	49	theme	glycosylation	926:938	arg1	motif					949:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	6	49	theme	glycosylation	926:938	arg1	identical					959:967	identical	959:967	identical	959:967	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	3	50	gly	glycosylated	602:613	arg1	which					537:541	which	537:541	which	537:541	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	3	50	gly	glycosylated	602:613	arg1	fraction					557:564	the major fraction	547:564	the major fraction of the EPS	547:575	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	8	51	gly	glycoproteins	1214:1226	arg1	glycoproteins					1214:1226	glycoproteins	1214:1226	glycoproteins	1214:1226	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	9	52	theme	different	1377:1385	arg1	glycoconjugates					1387:1401	different glycoconjugates	1377:1401	different glycoconjugates	1377:1401	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	8	53	theme	important	1196:1204	arg1	role					1206:1209	an important role	1193:1209	an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms	1193:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	6	54	theme	potential	916:924	arg1	motif					949:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif	912:953	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	6	54	theme	potential	916:924	arg1	identical					959:967	identical	959:967	identical	959:967	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	8	55	theme	multicellular	1339:1351	arg1	organisms					1353:1361	multicellular organisms	1339:1361	multicellular organisms	1339:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	9	56	theme	more	1476:1479	arg1	characterization					1490:1505	more in-depth characterization	1476:1505	more in-depth characterization of different glycoconjugates	1476:1534	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	3	57	theme	major	551:555	arg1	which					537:541	which	537:541	which	537:541	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	3	57	theme	major	551:555	arg1	fraction					557:564	the major fraction	547:564	the major fraction of the EPS	547:575	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	2	58	dep	formation	316:324	arg1	the					312:314	the	312:314	the	312:314	To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS).					
30335377	0	59	theme	Polymeric	60:68	arg1	Substances					70:79	Extracellular Polymeric Substances	46:79	Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge	46:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	9	60	theme	sludge	1453:1458	arg1	matrix					1434:1439	the matrix	1430:1439	the matrix	1430:1439	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	3	61	theme	research	483:490	arg1	aim					471:473	The aim	467:473	The aim of this research	467:490	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	9	62	theme	EPS	1546:1548	arg1	studies					1550:1556	future EPS studies	1539:1556	future EPS studies	1539:1556	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	6	63	theme	layer	1002:1006	arg1	protein					1008:1014	the surface layer protein	990:1014	the surface layer protein of " Candidatus Kuenenia stuttgartiensis"	990:1056	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	4	64	theme	gel	756:758	arg1	electrophoresis					760:774	denaturing polyacrylamide gel electrophoresis	730:774	denaturing polyacrylamide gel electrophoresis	730:774	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	1	65	from	wastewater	221:230	arg1	removal					208:214	efficient nitrogen removal	189:214	efficient nitrogen removal from wastewater	189:230	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	9	66	theme	in-depth	1481:1488	arg1	characterization					1490:1505	more in-depth characterization	1476:1505	more in-depth characterization of different glycoconjugates	1476:1534	Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.					
30335377	5	67	with	analysis	788:795	arg1	spectrometry					807:818	mass spectrometry	802:818	mass spectrometry	802:818	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	4	68	theme	polyacrylamide	741:754	arg1	electrophoresis					760:774	denaturing polyacrylamide gel electrophoresis	730:774	denaturing polyacrylamide gel electrophoresis	730:774	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	8	69	theme	comparable	1268:1277	arg1	granules					1258:1265	anammox granules	1250:1265	anammox granules	1250:1265	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	8	70	theme	glycans	1300:1306	arg1	importance					1286:1295	the importance	1282:1295	the importance of glycans in the extracellular matrix of multicellular organisms	1282:1361	Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms.					
30335377	1	71	theme	anammox	244:250	arg1	bacteria					252:259	anammox bacteria	244:259	anammox bacteria	244:259	ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules.					
30335377	0	72	theme	Full-Scale	84:93	arg1	Sludge					112:117	Full-Scale Anammox Granular Sludge	84:117	Full-Scale Anammox Granular Sludge	84:117	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	3	73	theme	EPS	573:575	arg1	which					537:541	which	537:541	which	537:541	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	3	73	theme	EPS	573:575	arg1	fraction					557:564	the major fraction	547:564	the major fraction of the EPS	547:575	The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated.					
30335377	6	74	theme	Kuenenia	1032:1039	arg1	stuttgartiensis					1041:1055	" Candidatus Kuenenia stuttgartiensis"	1019:1056	" Candidatus Kuenenia stuttgartiensis"	1019:1056	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	4	75	theme	anammox	651:657	arg1	sludge					668:673	full-scale anammox granular sludge	640:673	full-scale anammox granular sludge	640:673	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30335377	5	76	theme	abundant	828:835	arg1	glycoprotein					837:848	a high abundant glycoprotein	821:848	a high abundant glycoprotein	821:848	By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified.					
30335377	6	77	theme	surface	994:1000	arg1	protein					1008:1014	the surface layer protein	990:1014	the surface layer protein of " Candidatus Kuenenia stuttgartiensis"	990:1056	The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of " Candidatus Kuenenia stuttgartiensis".					
30335377	0	78	theme	Glycoproteins	18:30	arg1	Identification					0:13	Identification	0:13	Identification of Glycoproteins	0:30	Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.					
30335377	4	79	theme	full-scale	640:649	arg1	sludge					668:673	full-scale anammox granular sludge	640:673	full-scale anammox granular sludge	640:673	EPS were extracted from full-scale anammox granular sludge, dominated by " Candidatus Brocadia", and subjected to denaturing polyacrylamide gel electrophoresis.					
30959980	0	0	theme	Hyla	94:97	arg1	Mucin					117:121	Hyla Rabbit Intestinal Mucin	94:121	Hyla Rabbit Intestinal Mucin	94:121	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	7	1	theme	structural	1168:1177	arg1	characterization					1179:1194	These structural characterization	1162:1194	These structural characterization	1162:1194	These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.					
30959980	2	2	theme	intestinal	368:377	arg1	O-glycosylation					379:393	intestinal O-glycosylation	368:393	intestinal O-glycosylation	368:393	Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions.					
30959980	7	3	theme	hyla	1292:1295	arg1	rabbit					1297:1302	hyla rabbit	1292:1302	hyla rabbit	1292:1302	These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.					
30959980	0	4	theme	Intestinal	106:115	arg1	Mucin					117:121	Hyla Rabbit Intestinal Mucin	94:121	Hyla Rabbit Intestinal Mucin	94:121	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	3	5	theme	intestine	558:566	arg1	RIF6					537:540	RIF6	537:540	RIF6	537:540	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	5	theme	intestine	558:566	arg1	glycopeptide					523:534	a major mucin-type glycopeptide	504:534	a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine	504:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	1	6	theme	microbial	275:283	arg1	colonization					285:296	microbial colonization	275:296	microbial colonization	275:296	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	0	7	theme	Rabbit	99:104	arg1	Mucin					117:121	Hyla Rabbit Intestinal Mucin	94:121	Hyla Rabbit Intestinal Mucin	94:121	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	6	8	theme	rabbit	1137:1142	arg1	mucin					1155:1159	rabbit intestinal mucin	1137:1159	rabbit intestinal mucin	1137:1159	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	3	9	theme	mucin-type	512:521	arg1	RIF6					537:540	RIF6	537:540	RIF6	537:540	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	9	theme	mucin-type	512:521	arg1	glycopeptide					523:534	a major mucin-type glycopeptide	504:534	a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine	504:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	10	theme	hyla	546:549	arg1	intestine					558:566	hyla rabbit intestine	546:566	hyla rabbit intestine	546:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	1	11	theme	mucus	180:184	arg1	component					163:171	the major component	153:171	the major component of the mucus covering the epithelium of the gastrointestinal tract	153:238	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	7	12	theme	species	1206:1212	arg1	diversity					1214:1222	species diversity	1206:1222	species diversity	1206:1222	These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.					
30959980	5	13	gly	sialylated	893:902	arg1	O-glycans					904:912	sialylated O-glycans	893:912	sialylated O-glycans	893:912	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	3	14	theme	rabbit	551:556	arg1	intestine					558:566	hyla rabbit intestine	546:566	hyla rabbit intestine	546:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	7	15	theme	deeper	1240:1245	arg1	insights					1247:1254	deeper insights	1240:1254	deeper insights into explaining the adaptability of hyla rabbit to the environment	1240:1321	These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.					
30959980	7	16	theme	rabbit	1297:1302	arg1	adaptability					1276:1287	the adaptability	1272:1287	the adaptability of hyla rabbit to the environment	1272:1321	These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.					
30959980	3	17	gly	glycopeptide	523:534	arg1	intestine					558:566	hyla rabbit intestine	546:566	hyla rabbit intestine	546:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	17	gly	glycopeptide	523:534	arg2	RIF6					537:540	RIF6	537:540	RIF6	537:540	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	17	gly	glycopeptide	523:534	arg2	glycopeptide					523:534	a major mucin-type glycopeptide	504:534	a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine	504:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	6	18	located	detected	1125:1132	arg1	mucin					1155:1159	rabbit intestinal mucin	1137:1159	rabbit intestinal mucin	1137:1159	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	6	18	located	detected	1125:1132	arg2	glycans					1107:1113	sulfated-sialylated glycans	1087:1113	sulfated-sialylated glycans	1087:1113	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	6	18	located	detected	1125:1132	arg2	disufated					1073:1081	unique disufated	1066:1081	unique disufated	1066:1081	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	6	18	located	detected	1125:1132	arg2	glycans					1039:1045	Sulfated glycans	1030:1045	Sulfated glycans accounted for 58%	1030:1063	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	5	19	theme	Neu5Gc-containing	857:873	arg1	structures					875:884	Neu5Gc-containing structures	857:884	Neu5Gc-containing structures within sialylated O-glycans	857:912	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	5	20	from	that	969:972	arg1	different					954:962	different	954:962	different	954:962	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	2	21	theme	numbers	325:331	arg1	worldwide					333:341	large numbers worldwide	319:341	large numbers worldwide	319:341	Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions.					
30959980	2	22	theme	interactions	468:479	arg1	understanding					437:449	the understanding	433:449	the understanding of host-pathogen interactions	433:479	Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions.					
30959980	2	23	theme	large	319:323	arg1	numbers					325:331	large numbers	319:331	large numbers worldwide	319:341	Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions.					
30959980	5	24	theme	sialylated	893:902	arg1	O-glycans					904:912	sialylated O-glycans	893:912	sialylated O-glycans	893:912	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	2	25	theme	host-pathogen	454:466	arg1	interactions					468:479	host-pathogen interactions	454:479	host-pathogen interactions	454:479	Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions.					
30959980	6	26	gly	sulfated-sialylated	1087:1105	arg1	glycans					1107:1113	sulfated-sialylated glycans	1087:1113	sulfated-sialylated glycans	1087:1113	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	4	27	theme	Thirty-three	738:749	arg1	O-glycans					751:759	Thirty-three O-glycans	738:759	Thirty-three O-glycans	738:759	Thirty-three O-glycans were identified, and most of them were sulfated or sialylated glycans.					
30959980	0	28	theme	High	45:48	arg1	Neu5Gc					50:55	High Neu5Gc	45:55	High Neu5Gc	45:55	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	1	29	theme	gastrointestinal	217:232	arg1	tract					234:238	the gastrointestinal tract	213:238	the gastrointestinal tract	213:238	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	1	30	theme	tract	234:238	arg1	epithelium					199:208	the epithelium	195:208	the epithelium of the gastrointestinal tract	195:238	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	6	31	theme	unique	1066:1071	arg1	disufated					1073:1081	unique disufated	1066:1081	unique disufated	1066:1081	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	4	32	gly	sialylated	812:821	arg1	them					790:793	them	790:793	them	790:793	Thirty-three O-glycans were identified, and most of them were sulfated or sialylated glycans.					
30959980	4	32	gly	sialylated	812:821	arg1	most					782:785	most	782:785	most	782:785	Thirty-three O-glycans were identified, and most of them were sulfated or sialylated glycans.					
30959980	1	33	theme	Intestinal	124:133	arg1	mucins					135:140	Intestinal mucins	124:140	Intestinal mucins	124:140	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	0	34	theme	Neu5Gc	50:55	arg1	Profiling					0:8	Profiling	0:8	Profiling	0:8	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	0	34	theme	Neu5Gc	50:55	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	3	35	theme	bioinformatics	711:724	arg1	approaches					726:735	bioinformatics approaches	711:735	bioinformatics approaches	711:735	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	36	theme	liquid	639:644	arg1	LC-MS/MS					687:694	LC-MS/MS	687:694	LC-MS/MS	687:694	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	36	theme	liquid	639:644	arg1	spectrometry					673:684	liquid chromatography-tandem mass spectrometry	639:684	liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches	639:735	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	0	37	from	Mucin	117:121	arg1	Profiling					0:8	Profiling	0:8	Profiling	0:8	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	0	37	from	Mucin	117:121	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	3	38	theme	chromatography-tandem	646:666	arg1	LC-MS/MS					687:694	LC-MS/MS	687:694	LC-MS/MS	687:694	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	38	theme	chromatography-tandem	646:666	arg1	spectrometry					673:684	liquid chromatography-tandem mass spectrometry	639:684	liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches	639:735	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	5	39	theme	other	977:981	arg1	humans					1001:1006	humans	1001:1006	humans	1001:1006	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	5	39	theme	other	977:981	arg1	species					983:989	other species	977:989	other species including humans, mice, chickens, etc	977:1027	It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc.					
30959980	6	40	theme	Sulfated	1030:1037	arg1	glycans					1039:1045	Sulfated glycans	1030:1045	Sulfated glycans accounted for 58%	1030:1063	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	3	41	theme	mass	668:671	arg1	LC-MS/MS					687:694	LC-MS/MS	687:694	LC-MS/MS	687:694	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	41	theme	mass	668:671	arg1	spectrometry					673:684	liquid chromatography-tandem mass spectrometry	639:684	liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches	639:735	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	42	theme	major	506:510	arg1	RIF6					537:540	RIF6	537:540	RIF6	537:540	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	3	42	theme	major	506:510	arg1	glycopeptide					523:534	a major mucin-type glycopeptide	504:534	a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine	504:566	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	0	43	theme	O-glycans	79:87	arg1	Profiling					0:8	Profiling	0:8	Profiling	0:8	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	0	43	theme	O-glycans	79:87	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	3	44	theme	present	489:495	arg1	study					497:501	the present study	485:501	the present study	485:501	In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches.					
30959980	6	45	theme	sulfated-sialylated	1087:1105	arg1	glycans					1107:1113	sulfated-sialylated glycans	1087:1113	sulfated-sialylated glycans	1087:1113	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
30959980	1	46	theme	major	157:161	arg1	component					163:171	the major component	153:171	the major component of the mucus covering the epithelium of the gastrointestinal tract	153:238	Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization.					
30959980	0	47	theme	Sulfate-containing	60:77	arg1	O-glycans					79:87	Sulfate-containing O-glycans	60:87	Sulfate-containing O-glycans	60:87	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	0	48	theme	Structural	14:23	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.					
30959980	6	49	theme	intestinal	1144:1153	arg1	mucin					1155:1159	rabbit intestinal mucin	1137:1159	rabbit intestinal mucin	1137:1159	Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin.					
31554601	8	0	theme	fucosylation	1142:1153	arg1	levels					1155:1160	decreasing fucosylation levels	1131:1160	decreasing fucosylation levels	1131:1160	In comparison, when N-glycans are present, decreasing fucosylation levels enhanced the FcγR-binding affinity regardless of the types of N-glycans.					
31554601	4	1	theme	effector	547:554	arg1	functions					556:564	various immune effector functions	532:564	various immune effector functions	532:564	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	3	2	theme	different	479:487	arg1	isotypes					493:500	different IgG isotypes	479:500	different IgG isotypes	479:500	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	12	3	theme	N-glycan	1787:1794	arg1	patterns					1796:1803	antibody N-glycan patterns	1778:1803	antibody N-glycan patterns	1778:1803	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	8	4	theme	FcγR-binding	1175:1186	arg1	affinity					1188:1195	the FcγR-binding affinity	1171:1195	the FcγR-binding affinity regardless of the types of N-glycans	1171:1232	In comparison, when N-glycans are present, decreasing fucosylation levels enhanced the FcγR-binding affinity regardless of the types of N-glycans.					
31554601	4	5	theme	interactions	616:627	arg1	N-glycans					590:598	N-glycans	590:598	N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs	590:659	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	1	6	gly	glycoproteins	219:231	arg1	glycoproteins					219:231	glycoproteins	219:231	glycoproteins	219:231	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	11	7	theme	N-glycans	1575:1583	arg1	types					1566:1570	the types	1562:1570	the types of N-glycans	1562:1583	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	11	7	theme	N-glycans	1575:1583	arg1	levels					1518:1523	the levels	1514:1523	the levels of specific glycosylation	1514:1549	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	2	8	theme	IgG	287:289	arg1	function					275:282	The effector function	262:282	The effector function of IgG	262:289	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	12	9	from	understanding	1666:1678	arg1	general					1705:1711	general	1705:1711	general	1705:1711	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	10	10	theme	mIgG1	1437:1441	arg1	affinity					1425:1432	FcγR-binding affinity	1412:1432	FcγR-binding affinity of mIgG1	1412:1441	In conclusion, our findings clearly demonstrate that FcγR-binding affinity of mIgG1 is under the control of glycosylation.					
31554601	7	11	theme	mIgG1-FcγR	1068:1077	arg1	binding					1079:1085	mIgG1-FcγR binding	1068:1085	mIgG1-FcγR binding	1068:1085	The removal of N-glycans nearly completely abolished mIgG1-FcγR binding.					
31554601	4	12	theme	mIgG1	603:607	arg1	interactions					616:627	mIgG1 impact interactions	603:627	mIgG1 impact interactions between mIgG1s and murine FcγRs	603:659	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	9	13	theme	high-mannose	1248:1259	arg1	type					1261:1264	high-mannose type and hybrid type N-glycans	1248:1290	type	1261:1264	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	5	14	theme	processing	783:792	arg1	inhibitors					801:810	N-glycan processing enzyme inhibitors	774:810	N-glycan processing enzyme inhibitors	774:810	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	5	15	theme	hybridoma	819:827	arg1	media					837:841	the hybridoma culture media	815:841	the hybridoma culture media	815:841	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	7	16	theme	N-glycans	1030:1038	arg1	removal					1019:1025	The removal	1015:1025	The removal of N-glycans	1015:1038	The removal of N-glycans nearly completely abolished mIgG1-FcγR binding.					
31554601	5	17	theme	enzyme-linked	895:907	arg1	ELISA					930:934	ELISA	930:934	ELISA	930:934	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	5	17	theme	enzyme-linked	895:907	arg1	assay					923:927	enzyme-linked immunosorbent assay	895:927	enzyme-linked immunosorbent assay (ELISA) analysis	895:944	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	9	18	theme	type	1277:1280	arg1	N-glycans					1282:1290	high-mannose type and hybrid type N-glycans	1248:1290	N-glycans	1282:1290	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	5	19	theme	assay	923:927	arg1	analysis					937:944	enzyme-linked immunosorbent assay (ELISA) analysis	895:944	enzyme-linked immunosorbent assay (ELISA) analysis	895:944	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	12	20	theme	immune	1746:1751	arg1	responses					1753:1761	host immune responses	1741:1761	host immune responses	1741:1761	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	5	21	theme	immunosorbent	909:921	arg1	ELISA					930:934	ELISA	930:934	ELISA	930:934	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	5	21	theme	immunosorbent	909:921	arg1	assay					923:927	enzyme-linked immunosorbent assay	895:927	enzyme-linked immunosorbent assay (ELISA) analysis	895:944	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	4	22	theme	murine	648:653	arg1	FcγRs					655:659	murine FcγRs	648:659	murine FcγRs	648:659	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	5	23	theme	different	717:725	arg1	patterns					736:743	different N-glycan patterns	717:743	different N-glycan patterns	717:743	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	2	24	from	located	320:326	arg1	Fc					357:358	Fc	357:358	Fc	357:358	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	2	24	from	located	320:326	arg1	fragment					347:354	the crystalline fragment	331:354	the crystalline fragment (Fc)	331:359	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	10	25	theme	glycosylation	1467:1479	arg1	control					1456:1462	the control	1452:1462	the control of glycosylation	1452:1479	In conclusion, our findings clearly demonstrate that FcγR-binding affinity of mIgG1 is under the control of glycosylation.					
31554601	11	26	theme	glycosylation	1537:1549	arg1	types					1566:1570	the types	1562:1570	the types of N-glycans	1562:1583	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	11	26	theme	glycosylation	1537:1549	arg1	levels					1518:1523	the levels	1514:1523	the levels of specific glycosylation	1514:1549	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	3	27	theme	Fc	362:363	arg1	receptor					371:378	Fc gamma receptor	362:378	Fc gamma receptor	362:378	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	12	28	theme	N-glycans	1683:1691	arg1	function					1693:1700	N-glycans function	1683:1700	N-glycans function in general	1683:1711	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	1	29	theme	post-translational	138:155	arg1	modifications					157:169	the most common post-translational modifications	122:169	the most common post-translational modifications	122:169	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	3	30	dep	-binding	386:393	arg1	receptor					371:378	Fc gamma receptor	362:378	Fc gamma receptor	362:378	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	9	31	theme	complex	1335:1341	arg1	N-glycans					1348:1356	complex type N-glycans	1335:1356	complex type N-glycans	1335:1356	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	0	32	theme	FcγR-binding	0:11	arg1	affinity					13:20	FcγR-binding affinity	0:20	FcγR-binding affinity of monoclonal murine IgG1s	0:47	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	5	33	theme	enzyme	794:799	arg1	inhibitors					801:810	N-glycan processing enzyme inhibitors	774:810	N-glycan processing enzyme inhibitors	774:810	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	3	34	theme	effector	433:440	arg1	functions					442:450	the most important effector functions	414:450	the most important effector functions in IgG	414:457	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	0	35	theme	murine	36:41	arg1	IgG1s					43:47	monoclonal murine IgG1s	25:47	monoclonal murine IgG1s	25:47	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	3	36	theme	-binding	386:393	arg1	affinity					395:402	Fc gamma receptor (FcγR)-binding affinity	362:402	Fc gamma receptor (FcγR)-binding affinity	362:402	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	0	37	theme	N-linked	68:75	arg1	oligosaccharides					80:95	different N-linked Fc oligosaccharides	58:95	different N-linked Fc oligosaccharides	58:95	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	1	38	dep	structure	193:201	arg1	the					189:191	the	189:191	the	189:191	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	3	39	from	functions	442:450	arg1	IgG					455:457	IgG	455:457	IgG	455:457	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	12	40	theme	improved	1867:1874	arg1	characteristics					1876:1890	improved characteristics	1867:1890	improved characteristics	1867:1890	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	5	41	link	enzyme-linked	895:907	arg1	ELISA					930:934	ELISA	930:934	ELISA	930:934	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	5	41	link	enzyme-linked	895:907	arg1	assay					923:927	enzyme-linked immunosorbent assay	895:927	enzyme-linked immunosorbent assay (ELISA) analysis	895:944	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	3	42	theme	IgG	489:491	arg1	isotypes					493:500	different IgG isotypes	479:500	different IgG isotypes	479:500	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	5	43	theme	different	755:763	arg1	types					765:769	different types	755:769	different types of N-glycan processing enzyme inhibitors	755:810	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	8	44	theme	decreasing	1131:1140	arg1	levels					1155:1160	decreasing fucosylation levels	1131:1160	decreasing fucosylation levels	1131:1160	In comparison, when N-glycans are present, decreasing fucosylation levels enhanced the FcγR-binding affinity regardless of the types of N-glycans.					
31554601	2	45	from	fragment	347:354	arg1	located					320:326	located	320:326	located	320:326	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	4	46	theme	immune	540:545	arg1	functions					556:564	various immune effector functions	532:564	various immune effector functions	532:564	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	10	47	theme	FcγR-binding	1412:1423	arg1	affinity					1425:1432	FcγR-binding affinity	1412:1432	FcγR-binding affinity of mIgG1	1412:1441	In conclusion, our findings clearly demonstrate that FcγR-binding affinity of mIgG1 is under the control of glycosylation.					
31554601	2	48	theme	N-glycan	302:309	arg1	patterns					311:318	N-glycan patterns	302:318	N-glycan patterns located in the crystalline fragment (Fc)	302:359	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	12	49	from	function	1693:1700	arg1	general					1705:1711	general	1705:1711	general	1705:1711	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	5	50	theme	culture	829:835	arg1	media					837:841	the hybridoma culture media	815:841	the hybridoma culture media	815:841	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	4	51	theme	impact	609:614	arg1	interactions					616:627	mIgG1 impact interactions	603:627	mIgG1 impact interactions between mIgG1s and murine FcγRs	603:659	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	12	52	theme	antibody	1778:1785	arg1	patterns					1796:1803	antibody N-glycan patterns	1778:1803	antibody N-glycan patterns	1778:1803	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	2	53	theme	located	320:326	arg1	patterns					311:318	N-glycan patterns	302:318	N-glycan patterns located in the crystalline fragment (Fc)	302:359	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	1	54	theme	glycoproteins	219:231	arg1	function					207:214	function	207:214	function	207:214	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	1	54	theme	glycoproteins	219:231	arg1	structure					193:201	structure	193:201	structure	193:201	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	1	55	theme	immunoglobulin	238:251	arg1	IgG					256:258	IgG	256:258	IgG	256:258	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	1	55	theme	immunoglobulin	238:251	arg1	G					253:253	immunoglobulin G	238:253	immunoglobulin G (IgG)	238:259	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	5	56	with	mIgG1s	705:710	arg1	patterns					736:743	different N-glycan patterns	717:743	different N-glycan patterns	717:743	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	8	57	theme	N-glycans	1224:1232	arg1	types					1215:1219	the types	1211:1219	the types of N-glycans	1211:1232	In comparison, when N-glycans are present, decreasing fucosylation levels enhanced the FcγR-binding affinity regardless of the types of N-glycans.					
31554601	9	58	theme	hybrid	1270:1275	arg1	N-glycans					1282:1290	high-mannose type and hybrid type N-glycans	1248:1290	N-glycans	1282:1290	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	1	59	theme	common	131:136	arg1	modifications					157:169	the most common post-translational modifications	122:169	the most common post-translational modifications	122:169	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	0	60	theme	Fc	77:78	arg1	oligosaccharides					80:95	different N-linked Fc oligosaccharides	58:95	different N-linked Fc oligosaccharides	58:95	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	2	61	theme	effector	266:273	arg1	function					275:282	The effector function	262:282	The effector function of IgG	262:289	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	5	62	theme	inhibitors	801:810	arg1	types					765:769	different types	755:769	different types of N-glycan processing enzyme inhibitors	755:810	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	1	63	theme	modifications	157:169	arg1	one					115:117	one	115:117	one	115:117	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	1	63	theme	modifications	157:169	arg1	modifications					157:169	the most common post-translational modifications	122:169	the most common post-translational modifications	122:169	Glycosylation is one of the most common post-translational modifications which diversifies the structure and function of glycoproteins like immunoglobulin G (IgG).					
31554601	12	64	theme	host	1741:1744	arg1	responses					1753:1761	host immune responses	1741:1761	host immune responses	1741:1761	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	12	65	from	general	1705:1711	arg1	understanding					1666:1678	our understanding	1662:1678	our understanding of N-glycans function in general	1662:1711	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	11	66	theme	specific	1528:1535	arg1	glycosylation					1537:1549	specific glycosylation	1528:1549	specific glycosylation	1528:1549	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	5	67	theme	N-glycan	727:734	arg1	patterns					736:743	different N-glycan patterns	717:743	different N-glycan patterns	717:743	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	9	68	theme	FcγR-binding	1300:1311	arg1	affinity					1313:1320	FcγR-binding affinity	1300:1320	FcγR-binding affinity	1300:1320	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	5	69	theme	FcγR-binding	867:878	arg1	affinity					880:887	their FcγR-binding affinity	861:887	their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis	861:944	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	6	70	theme	mIgG1	990:994	arg1	affinity					996:1003	mIgG1 affinity	990:1003	mIgG1 affinity to FcγRs	990:1012	We showed that N-glycans critically affect mIgG1 affinity to FcγRs.					
31554601	3	71	theme	gamma	365:369	arg1	receptor					371:378	Fc gamma receptor	362:378	Fc gamma receptor	362:378	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	4	72	dep	triggers	523:530	arg1	remains					661:667	remains	661:667	remains largely unknown	661:683	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	11	73	dep	levels	1518:1523	arg1	both					1509:1512	both	1509:1512	both	1509:1512	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	3	74	theme	important	423:431	arg1	functions					442:450	the most important effector functions	414:450	the most important effector functions in IgG	414:457	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	9	75	theme	type	1343:1346	arg1	N-glycans					1348:1356	complex type N-glycans	1335:1356	complex type N-glycans	1335:1356	Furthermore, high-mannose type and hybrid type N-glycans reduced FcγR-binding affinity, compared to complex type N-glycans.					
31554601	12	76	theme	function	1693:1700	arg1	understanding					1666:1678	our understanding	1662:1678	our understanding of N-glycans function in general	1662:1711	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	0	77	theme	monoclonal	25:34	arg1	IgG1s					43:47	monoclonal murine IgG1s	25:47	monoclonal murine IgG1s	25:47	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	5	78	theme	N-glycan	774:781	arg1	inhibitors					801:810	N-glycan processing enzyme inhibitors	774:810	N-glycan processing enzyme inhibitors	774:810	Here, we generated mIgG1s with different N-glycan patterns by adding different types of N-glycan processing enzyme inhibitors to the hybridoma culture media, before comparing their FcγR-binding affinity using enzyme-linked immunosorbent assay (ELISA) analysis.					
31554601	4	79	theme	various	532:538	arg1	functions					556:564	various immune effector functions	532:564	various immune effector functions	532:564	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	0	80	theme	IgG1s	43:47	arg1	affinity					13:20	FcγR-binding affinity	0:20	FcγR-binding affinity of monoclonal murine IgG1s	0:47	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	4	81	theme	Murine	503:508	arg1	IgG1					510:513	Murine IgG1	503:513	Murine IgG1 (mIgG1)	503:521	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	4	81	theme	Murine	503:508	arg1	mIgG1					516:520	mIgG1	516:520	mIgG1	516:520	Murine IgG1 (mIgG1) triggers various immune effector functions via FcγRs, however, how N-glycans of mIgG1 impact interactions between mIgG1s and murine FcγRs remains largely unknown.					
31554601	3	82	theme	functions	442:450	arg1	functions					442:450	the most important effector functions	414:450	the most important effector functions in IgG	414:457	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	3	82	theme	functions	442:450	arg1	one					407:409	one	407:409	one	407:409	Fc gamma receptor (FcγR)-binding affinity is one of the most important effector functions in IgG, and it varies with different IgG isotypes.					
31554601	0	83	theme	different	58:66	arg1	oligosaccharides					80:95	different N-linked Fc oligosaccharides	58:95	different N-linked Fc oligosaccharides	58:95	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	12	84	theme	therapeutic	1839:1849	arg1	antibodies					1851:1860	therapeutic antibodies	1839:1860	therapeutic antibodies	1839:1860	Together, these insights should greatly expand our understanding of N-glycans function in general, and assist in manipulating host immune responses by controlling antibody N-glycan patterns, which is important for designing therapeutic antibodies with improved characteristics.					
31554601	11	85	theme	FcγR-binding	1592:1603	arg1	affinity					1605:1612	FcγR-binding affinity	1592:1612	FcγR-binding affinity	1592:1612	Importantly, we found that both the levels of specific glycosylation as well as the types of N-glycans affect FcγR-binding affinity.					
31554601	0	86	link	N-linked	68:75	arg1	oligosaccharides					80:95	different N-linked Fc oligosaccharides	58:95	different N-linked Fc oligosaccharides	58:95	FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides.					
31554601	2	87	theme	crystalline	335:345	arg1	Fc					357:358	Fc	357:358	Fc	357:358	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
31554601	2	87	theme	crystalline	335:345	arg1	fragment					347:354	the crystalline fragment	331:354	the crystalline fragment (Fc)	331:359	The effector function of IgG depends on N-glycan patterns located in the crystalline fragment (Fc).					
30776362	0	0	theme	glycosylation	78:90	arg1	patients					92:99	glycosylation patients	78:99	glycosylation patients	78:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	5	1	theme	p.Gly536Val	1085:1095	arg1	mutation					1097:1104	a previously undescribed p.Gly536Val mutation	1060:1104	a previously undescribed p.Gly536Val mutation	1060:1104	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	1	2	from	apparatus	309:317	arg1	processing					274:283	the processing	270:283	the processing of glycans in the Golgi apparatus	270:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	5	3	theme	Total	863:867	arg1	N-glycans					869:877	Total N-glycans	863:877	Total N-glycans from fifteen CDG-II patients	863:906	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	7	4	theme	concentrated	1414:1425	arg1	structures					1453:1462	less concentrated yet biologically relevant structures	1409:1462	less concentrated yet biologically relevant structures	1409:1462	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	7	5	theme	other	1527:1531	arg1	subtypes					1540:1547	other CDG-II subtypes	1527:1547	other CDG-II subtypes	1527:1547	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	6	6	theme	mass	1298:1301	arg1	strategy					1315:1322	a strategy	1313:1322	a strategy that lowers the variability of minor components	1313:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	6	6	theme	mass	1298:1301	arg1	spectrum					1303:1310	a mass spectrum	1296:1310	a mass spectrum	1296:1310	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	0	7	from	quantification	9:22	arg1	disorder					66:73	type II congenital disorder	47:73	type II congenital disorder of glycosylation patients	47:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	1	8	theme	Glycosylation	166:178	arg1	Disorders					153:161	BACKGROUND Type II Congenital Disorders	123:161	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II)	123:187	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	8	theme	Glycosylation	166:178	arg1	diseases					204:211	diseases	204:211	diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	204:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	8	theme	Glycosylation	166:178	arg1	group					195:199	a group	193:199	a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	193:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	7	9	theme	CDG-II	1533:1538	arg1	subtypes					1540:1547	other CDG-II subtypes	1527:1547	other CDG-II subtypes	1527:1547	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	7	10	from	uncovering	1485:1494	arg1	subtypes					1540:1547	other CDG-II subtypes	1527:1547	other CDG-II subtypes	1527:1547	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	7	11	theme	relevant	1444:1451	arg1	structures					1453:1462	less concentrated yet biologically relevant structures	1409:1462	less concentrated yet biologically relevant structures	1409:1462	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	5	12	theme	2ATP6V0A2-CDG	988:1000	arg1	siblings					1002:1009	2ATP6V0A2-CDG siblings	988:1009	2ATP6V0A2-CDG siblings	988:1009	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	13	dep	RESULTS	855:861	arg1	considered					966:975	considered	966:975	were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation	961:1104	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	13	dep	RESULTS	855:861	arg1	evaluated					913:921	evaluated	913:921	were evaluated	908:921	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	4	14	theme	METHODS	582:588	arg1	samples					597:603	METHODS Plasma samples	582:603	METHODS Plasma samples from control individuals	582:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	14	theme	METHODS	582:588	arg1	standards					713:721	internal standards	704:721	internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses	704:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	0	15	theme	patients	92:99	arg1	disorder					66:73	type II congenital disorder	47:73	type II congenital disorder of glycosylation patients	47:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	5	16	theme	them	1046:1049	arg1	them					1046:1049	them	1046:1049	them	1046:1049	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	16	theme	them	1046:1049	arg1	one					1039:1041	one	1039:1041	one	1039:1041	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	16	theme	them	1046:1049	arg1	patients					1029:1036	2 MAN1B1-CDG patients	1016:1036	2 MAN1B1-CDG patients	1016:1036	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	4	17	theme	-MS/MS	838:843	arg1	analyses					845:852	-MS/MS analyses	838:852	-MS/MS analyses	838:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	18	theme	internal	704:711	arg1	samples					597:603	METHODS Plasma samples	582:603	METHODS Plasma samples from control individuals	582:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	18	theme	internal	704:711	arg1	standards					713:721	internal standards	704:721	internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses	704:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	3	19	theme	N-glycan	462:469	arg1	structures					471:480	specific N-glycan structures	453:480	specific N-glycan structures	453:480	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	5	20	contain	carrying	1051:1058	arg1	them					1046:1049	them	1046:1049	them	1046:1049	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	20	contain	carrying	1051:1058	arg2	mutation					1097:1104	a previously undescribed p.Gly536Val mutation	1060:1104	a previously undescribed p.Gly536Val mutation	1060:1104	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	20	contain	carrying	1051:1058	arg1	one					1039:1041	one	1039:1041	one	1039:1041	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	20	contain	carrying	1051:1058	arg1	patients					1029:1036	2 MAN1B1-CDG patients	1016:1036	2 MAN1B1-CDG patients	1016:1036	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	6	21	theme	minor	1355:1359	arg1	components					1361:1370	minor components	1355:1370	minor components	1355:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	1	22	from	glycans	288:294	arg1	apparatus					309:317	the Golgi apparatus	299:317	the Golgi apparatus	299:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	3	23	from	changes	503:509	arg1	levels					523:528	relative levels	514:528	relative levels	514:528	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	6	24	theme	feasible	1144:1151	arg1	alternative					1153:1163	a feasible alternative	1142:1163	a feasible alternative	1142:1163	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	5	25	theme	CDG-II	892:897	arg1	patients					899:906	fifteen CDG-II patients	884:906	fifteen CDG-II patients	884:906	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	0	26	theme	mass	104:107	arg1	spectrometry					109:120	mass spectrometry	104:120	mass spectrometry	104:120	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	3	27	theme	relative	514:521	arg1	levels					523:528	relative levels	514:528	relative levels	514:528	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	3	28	theme	CDG-II	417:422	arg1	subtypes					424:431	some CDG-II subtypes	412:431	some CDG-II subtypes	412:431	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	0	29	from	N-glycans	34:42	arg1	disorder					66:73	type II congenital disorder	47:73	type II congenital disorder of glycosylation patients	47:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	1	30	from	processing	274:283	arg1	apparatus					309:317	the Golgi apparatus	299:317	the Golgi apparatus	299:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	5	31	from	patients	899:906	arg1	N-glycans					869:877	Total N-glycans	863:877	Total N-glycans from fifteen CDG-II patients	863:906	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	6	32	theme	summed	1275:1280	arg1	signal					1282:1287	the total summed signal	1265:1287	the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components	1265:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	0	33	theme	Relative	0:7	arg1	quantification					9:22	Relative quantification	0:22	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.	0:121	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	6	34	theme	glycan	1231:1236	arg1	fractions					1252:1260	fractions	1252:1260	fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components	1252:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	6	34	theme	glycan	1231:1236	arg1	structures					1238:1247	glycan structures	1231:1247	glycan structures	1231:1247	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	4	35	theme	MALDI	818:822	arg1	MS					824:825	MALDI MS	818:825	MALDI MS	818:825	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	2	36	theme	valuable	354:361	arg1	tool					363:366	a valuable tool	352:366	a valuable tool in the definition of CDG-II subtypes	352:403	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	2	36	theme	valuable	354:361	arg1	Spectrometry					325:336	Mass Spectrometry	320:336	Mass Spectrometry (MS)	320:341	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	0	37	theme	plasma	27:32	arg1	N-glycans					34:42	plasma N-glycans	27:42	plasma N-glycans in type II congenital disorder of glycosylation patients	27:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	4	38	used	used	696:699	arg2	standards					713:721	internal standards	704:721	internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses	704:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	38	used	used	696:699	arg2	samples					597:603	METHODS Plasma samples	582:603	METHODS Plasma samples from control individuals	582:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	2	39	theme	subtypes	396:403	arg1	definition					375:384	the definition	371:384	the definition of CDG-II subtypes	371:403	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	1	40	theme	glycans	288:294	arg1	processing					274:283	the processing	270:283	the processing of glycans in the Golgi apparatus	270:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	41	with	diseases	204:211	arg1	diagnostics					230:240	challenging diagnostics	218:240	challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	218:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	7	42	theme	novel	1499:1503	arg1	glycans					1516:1522	novel diagnostic glycans	1499:1522	novel diagnostic glycans	1499:1522	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	4	43	from	individuals	618:628	arg1	samples					597:603	METHODS Plasma samples	582:603	METHODS Plasma samples from control individuals	582:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	43	from	individuals	618:628	arg1	standards					713:721	internal standards	704:721	internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses	704:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	2	44	theme	CDG-II	389:394	arg1	subtypes					396:403	CDG-II subtypes	389:403	CDG-II subtypes	389:403	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	7	45	theme	diagnostic	1505:1514	arg1	glycans					1516:1522	novel diagnostic glycans	1499:1522	novel diagnostic glycans	1499:1522	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	4	46	theme	deuterated	660:669	arg1	I-CD3					684:688	I-CD3	684:688	I-CD3	684:688	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	46	theme	deuterated	660:669	arg1	iodomethane					671:681	deuterated iodomethane	660:681	deuterated iodomethane (I-CD3)	660:689	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	0	47	theme	N-glycans	34:42	arg1	quantification					9:22	Relative quantification	0:22	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.	0:121	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	1	48	from	defects	259:265	arg1	processing					274:283	the processing	270:283	the processing of glycans in the Golgi apparatus	270:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	0	49	from	disorder	66:73	arg1	quantification					9:22	Relative quantification	0:22	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.	0:121	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	2	50	from	tool	363:366	arg1	definition					375:384	the definition	371:384	the definition of CDG-II subtypes	371:403	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	1	51	theme	diseases	204:211	arg1	group					195:199	a group	193:199	a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	193:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	51	theme	diseases	204:211	arg1	Disorders					153:161	BACKGROUND Type II Congenital Disorders	123:161	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II)	123:187	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	51	theme	diseases	204:211	arg1	diseases					204:211	diseases	204:211	diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	204:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	5	52	with	cases	930:934	arg1	diagnosis					951:959	molecular diagnosis	941:959	molecular diagnosis	941:959	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	1	53	theme	Golgi	303:307	arg1	apparatus					309:317	the Golgi apparatus	299:317	the Golgi apparatus	299:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	6	54	theme	signal	1282:1287	arg1	fractions					1252:1260	fractions	1252:1260	fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components	1252:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	6	54	theme	signal	1282:1287	arg1	structures					1238:1247	glycan structures	1231:1247	glycan structures	1231:1247	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	3	55	theme	quantification	558:571	arg1	methods					573:579	quantification methods	558:579	quantification methods	558:579	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	7	56	theme	glycans	1516:1522	arg1	uncovering					1485:1494	the uncovering	1481:1494	the uncovering of novel diagnostic glycans in other CDG-II subtypes	1481:1547	Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes.					
30776362	0	57	theme	type	47:50	arg1	disorder					66:73	type II congenital disorder	47:73	type II congenital disorder of glycosylation patients	47:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	6	58	theme	components	1361:1370	arg1	variability					1340:1350	the variability	1336:1350	the variability of minor components	1336:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	3	59	theme	specific	453:460	arg1	structures					471:480	specific N-glycan structures	453:480	specific N-glycan structures	453:480	While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods.					
30776362	6	60	theme	CDG-II	1192:1197	arg1	diagnosis					1199:1207	CDG-II diagnosis	1192:1207	CDG-II diagnosis by MS	1192:1213	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	1	61	theme	BACKGROUND	123:132	arg1	Disorders					153:161	BACKGROUND Type II Congenital Disorders	123:161	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II)	123:187	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	61	theme	BACKGROUND	123:132	arg1	diseases					204:211	diseases	204:211	diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	204:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	61	theme	BACKGROUND	123:132	arg1	group					195:199	a group	193:199	a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	193:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	6	62	theme	total	1269:1273	arg1	signal					1282:1287	the total summed signal	1265:1287	the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components	1265:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	1	63	theme	Type	134:137	arg1	Disorders					153:161	BACKGROUND Type II Congenital Disorders	123:161	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II)	123:187	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	63	theme	Type	134:137	arg1	diseases					204:211	diseases	204:211	diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	204:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	63	theme	Type	134:137	arg1	group					195:199	a group	193:199	a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	193:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	5	64	theme	molecular	941:949	arg1	diagnosis					951:959	molecular diagnosis	941:959	molecular diagnosis	941:959	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	2	65	theme	Mass	320:323	arg1	MS					339:340	MS	339:340	MS	339:340	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	2	65	theme	Mass	320:323	arg1	Spectrometry					325:336	Mass Spectrometry	320:336	Mass Spectrometry (MS)	320:341	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	2	65	theme	Mass	320:323	arg1	tool					363:366	a valuable tool	352:366	a valuable tool in the definition of CDG-II subtypes	352:403	Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes.					
30776362	1	66	theme	challenging	218:228	arg1	diagnostics					230:240	challenging diagnostics	218:240	challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	218:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	5	67	theme	MAN1B1-CDG	1018:1027	arg1	them					1046:1049	them	1046:1049	them	1046:1049	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	67	theme	MAN1B1-CDG	1018:1027	arg1	one					1039:1041	one	1039:1041	one	1039:1041	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	5	67	theme	MAN1B1-CDG	1018:1027	arg1	patients					1029:1036	2 MAN1B1-CDG patients	1016:1036	2 MAN1B1-CDG patients	1016:1036	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	6	68	theme	current	1172:1178	arg1	methods					1180:1186	the current methods	1168:1186	the current methods for CDG-II diagnosis by MS	1168:1213	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	1	69	theme	Congenital	142:151	arg1	Disorders					153:161	BACKGROUND Type II Congenital Disorders	123:161	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II)	123:187	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	69	theme	Congenital	142:151	arg1	diseases					204:211	diseases	204:211	diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	204:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	1	69	theme	Congenital	142:151	arg1	group					195:199	a group	193:199	a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus	193:317	BACKGROUND Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus.					
30776362	6	70	dep	CONCLUSIONS	1107:1117	arg1	offers					1135:1140	offers	1135:1140	offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components	1135:1370	CONCLUSIONS Our methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components.					
30776362	4	71	theme	control	610:616	arg1	individuals					618:628	control individuals	610:628	control individuals	610:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	0	72	theme	congenital	55:64	arg1	disorder					66:73	type II congenital disorder	47:73	type II congenital disorder of glycosylation patients	47:99	Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry.					
30776362	5	73	theme	undescribed	1073:1083	arg1	mutation					1097:1104	a previously undescribed p.Gly536Val mutation	1060:1104	a previously undescribed p.Gly536Val mutation	1060:1104	RESULTS Total N-glycans from fifteen CDG-II patients were evaluated, and 4 cases with molecular diagnosis were considered in detail: 2ATP6V0A2-CDG siblings, and 2 MAN1B1-CDG patients, one of them carrying a previously undescribed p.Gly536Val mutation.					
30776362	4	74	theme	Plasma	590:595	arg1	samples					597:603	METHODS Plasma samples	582:603	METHODS Plasma samples from control individuals	582:628	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	74	theme	Plasma	590:595	arg1	standards					713:721	internal standards	704:721	internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses	704:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30776362	4	75	dep	derivatized	768:778	arg1	followed					806:813	followed	806:813	followed by MALDI MS, LC-MS and -MS/MS analyses	806:852	METHODS Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3), followed by MALDI MS, LC-MS and -MS/MS analyses.					
30802037	8	0	theme	decreased	1271:1279	arg1	space					1288:1292	decreased search space	1271:1292	decreased search space	1271:1292	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	6	1	theme	combinations	1041:1052	arg1	mass					1024:1027	every possible summed mass	1002:1027	every possible summed mass of O-glycan combinations on at most three S/T residues	1002:1082	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	7	2	theme	protein	1159:1165	arg1	database					1176:1183	the protein sequence database	1155:1183	the protein sequence database	1155:1183	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	10	3	theme	more	1641:1644	arg1	structures					1653:1662	more glycan structures	1641:1662	more glycan structures	1641:1662	It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.					
30802037	6	4	theme	O-glycan	1032:1039	arg1	combinations					1041:1052	O-glycan combinations	1032:1052	O-glycan combinations	1032:1052	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	6	5	theme	precursor	949:957	arg1	mass					959:962	The precursor mass	945:962	The precursor mass of the MS/MS spectrum	945:984	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	8	6	contain	had	1298:1300	arg1	method					1255:1260	this method	1250:1260	this method	1250:1260	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	8	6	contain	had	1298:1300	arg2	sensitivity					1312:1322	excellent sensitivity	1302:1322	excellent sensitivity	1302:1322	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	6	7	from	mass	1024:1027	arg1	residues					1075:1082	at most three S/T residues	1057:1082	at most three S/T residues	1057:1082	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	9	8	theme	peptide	1531:1537	arg1	identification					1548:1561	peptide sequence identification	1531:1561	peptide sequence identification	1531:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	9	theme	conventional	1384:1395	arg1	approach					1407:1414	the conventional searching approach	1380:1414	the conventional searching approach	1380:1414	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	10	10	theme	O-glycosylation	1712:1726	arg1	microheterogeneity					1690:1707	microheterogeneity	1690:1707	microheterogeneity of O-glycosylation	1690:1726	It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.					
30802037	9	11	theme	matching	1488:1495	arg1	spectra					1480:1486	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	4	12	theme	novel	740:744	arg1	approach					746:753	a novel approach	738:753	a novel approach	738:753	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	9	13	theme	79	1448:1449	arg1	%					1450:1450	%	1450:1450	%	1450:1450	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	3	14	from	tags	648:651	arg1	residues					668:675	the Ser/Thr residues	656:675	the Ser/Thr residues	656:675	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	3	15	theme	carbohydrate-related	497:516	arg1	tag					518:520	virtually no carbohydrate-related tag	484:520	virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD	484:594	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	4	16	theme	spectra	821:827	arg1	interpretation					784:797	the interpretation	780:797	the interpretation of O-glycopeptide HCD spectra	780:827	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	5	17	from	tags	867:870	arg1	residues					887:894	the Ser/Thr residues	875:894	the Ser/Thr residues	875:894	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	5	18	theme	peptide	930:936	arg1	level					938:942	the peptide level	926:942	the peptide level	926:942	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	6	19	theme	summed	1017:1022	arg1	mass					1024:1027	every possible summed mass	1002:1027	every possible summed mass of O-glycan combinations on at most three S/T residues	1002:1082	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	8	20	theme	search	1281:1286	arg1	space					1288:1292	decreased search space	1271:1292	decreased search space	1271:1292	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	9	21	theme	searching	1397:1405	arg1	approach					1407:1414	the conventional searching approach	1380:1414	the conventional searching approach	1380:1414	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	22	gly	glycopeptide	1467:1478	arg2	glycopeptide					1467:1478	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	2	23	gly	O-glycopeptides	394:408	arg2	O-glycopeptides					394:408	intact mucin-type O-glycopeptides	376:408	intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation	376:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	7	24	with	spectra	1093:1099	arg1	masses					1126:1131	these new precursor masses	1106:1131	these new precursor masses	1106:1131	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	2	25	theme	mucin-type	383:392	arg1	O-glycopeptides					394:408	intact mucin-type O-glycopeptides	376:408	intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation	376:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	2	26	theme	glycosylation	452:464	arg1	microheterogeneity					426:443	the high microheterogeneity	417:443	the high microheterogeneity of the glycosylation	417:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	7	27	theme	sequence	1167:1174	arg1	database					1176:1183	the protein sequence database	1155:1183	the protein sequence database	1155:1183	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	7	28	theme	new	1112:1114	arg1	masses					1126:1131	these new precursor masses	1106:1131	these new precursor masses	1106:1131	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	2	29	theme	intact	376:381	arg1	O-glycopeptides					394:408	intact mucin-type O-glycopeptides	376:408	intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation	376:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	7	30	theme	precursor	1116:1124	arg1	masses					1126:1131	these new precursor masses	1106:1131	these new precursor masses	1106:1131	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	9	31	from	improvement	1452:1462	arg1	spectra					1480:1486	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	31	from	improvement	1452:1462	arg1	identification					1548:1561	peptide sequence identification	1531:1561	peptide sequence identification	1531:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	31	from	improvement	1452:1462	arg1	identification					1511:1524	glycopeptide identification	1498:1524	glycopeptide identification	1498:1524	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	3	32	theme	Ser/Thr	660:666	arg1	residues					668:675	the Ser/Thr residues	656:675	the Ser/Thr residues	656:675	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	9	33	from	%	1441:1441	arg1	spectra					1480:1486	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	33	from	%	1441:1441	arg1	identification					1548:1561	peptide sequence identification	1531:1561	peptide sequence identification	1531:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	33	from	%	1441:1441	arg1	identification					1511:1524	glycopeptide identification	1498:1524	glycopeptide identification	1498:1524	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	5	34	theme	variable	904:911	arg1	tags					918:921	variable mass tags	904:921	variable mass tags	904:921	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	1	35	theme	variable	199:206	arg1	modification					208:219	a variable modification	197:219	a variable modification on the specific residue(s)	197:246	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	10	36	theme	structures	1653:1662	arg1	consideration					1624:1636	the consideration	1620:1636	the consideration of more glycan structures	1620:1662	It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.					
30802037	1	37	theme	modified	275:282	arg1	peptides					284:291	the modified peptides	271:291	the modified peptides for database searching	271:314	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	10	38	theme	glycan	1646:1651	arg1	structures					1653:1662	more glycan structures	1641:1662	more glycan structures	1641:1662	It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.					
30802037	3	39	from	tag	518:520	arg1	fragments					537:545	the peptide fragments	525:545	the peptide fragments after the O-glycopeptides are dissociated in HCD	525:594	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	5	40	theme	mass	913:916	arg1	tags					918:921	variable mass tags	904:921	variable mass tags	904:921	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	9	41	theme	sequence	1539:1546	arg1	identification					1548:1561	peptide sequence identification	1531:1561	peptide sequence identification	1531:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	2	42	theme	high	421:424	arg1	microheterogeneity					426:443	the high microheterogeneity	417:443	the high microheterogeneity of the glycosylation	417:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	0	43	theme	Searching	6:14	arg1	Strategy					16:23	A New Searching Strategy	0:23	A New Searching Strategy for the Identification of O-Linked Glycopeptides	0:72	A New Searching Strategy for the Identification of O-Linked Glycopeptides.					
30802037	9	44	gly	glycopeptide	1498:1509	arg2	glycopeptide					1498:1509	glycopeptide identification	1498:1524	glycopeptide identification	1498:1524	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	8	45	theme	excellent	1302:1310	arg1	sensitivity					1312:1322	excellent sensitivity	1302:1322	excellent sensitivity	1302:1322	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	1	46	theme	homogeneous	95:105	arg1	phosphorylation					156:170	protein phosphorylation	148:170	protein phosphorylation	148:170	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	1	46	theme	homogeneous	95:105	arg1	modifications					126:138	homogeneous post-translational modifications	95:138	homogeneous post-translational modifications such as protein phosphorylation and acetylation	95:186	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	1	46	theme	homogeneous	95:105	arg1	acetylation					176:186	acetylation	176:186	acetylation	176:186	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	9	47	theme	glycopeptide	1467:1478	arg1	spectra					1480:1486	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	3	48	theme	peptide	529:535	arg1	fragments					537:545	the peptide fragments	525:545	the peptide fragments after the O-glycopeptides are dissociated in HCD	525:594	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	6	49	theme	S/T	1071:1073	arg1	residues					1075:1082	at most three S/T residues	1057:1082	at most three S/T residues	1057:1082	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	8	50	contain	had	1262:1264	arg2	space					1288:1292	decreased search space	1271:1292	decreased search space	1271:1292	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	8	50	contain	had	1262:1264	arg1	method					1255:1260	this method	1250:1260	this method	1250:1260	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	7	51	theme	variable	1201:1208	arg1	modifications					1217:1229	variable glycan modifications	1201:1229	variable glycan modifications	1201:1229	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	8	52	theme	O-glycopeptides	1349:1363	arg1	identification					1331:1344	the identification	1327:1344	the identification of O-glycopeptides	1327:1363	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	5	53	theme	variable	853:860	arg1	tags					867:870	the variable mass tags	849:870	the variable mass tags on the Ser/Thr residues	849:894	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	6	54	theme	spectrum	977:984	arg1	mass					959:962	The precursor mass	945:962	The precursor mass of the MS/MS spectrum	945:984	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	1	55	theme	database	297:304	arg1	searching					306:314	database searching	297:314	database searching	297:314	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	3	56	theme	variable	634:641	arg1	tags					648:651	the variable mass tags	630:651	the variable mass tags on the Ser/Thr residues	630:675	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	1	57	theme	specific	228:235	arg1	s					245:245	the specific residue(s)	224:246	the specific residue(s)	224:246	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	5	58	theme	mass	862:865	arg1	tags					867:870	the variable mass tags	849:870	the variable mass tags on the Ser/Thr residues	849:894	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	3	59	theme	mass	643:646	arg1	tags					648:651	the variable mass tags	630:651	the variable mass tags on the Ser/Thr residues	630:675	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	4	60	theme	O-glycopeptide	802:815	arg1	spectra					821:827	O-glycopeptide HCD spectra	802:827	O-glycopeptide HCD spectra	802:827	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	1	61	theme	post-translational	107:124	arg1	phosphorylation					156:170	protein phosphorylation	148:170	protein phosphorylation	148:170	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	1	61	theme	post-translational	107:124	arg1	modifications					126:138	homogeneous post-translational modifications	95:138	homogeneous post-translational modifications such as protein phosphorylation and acetylation	95:186	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	1	61	theme	post-translational	107:124	arg1	acetylation					176:186	acetylation	176:186	acetylation	176:186	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	4	62	attach	present	730:736	arg2	we					727:728	we	727:728	we	727:728	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	4	62	attach	present	730:736	arg1	study					720:724	this study	715:724	this study	715:724	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	10	63	gly	microheterogeneity	1690:1707	arg1	O-glycosylation					1712:1726	O-glycosylation	1712:1726	O-glycosylation	1712:1726	It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.					
30802037	4	64	theme	HCD	817:819	arg1	spectra					821:827	O-glycopeptide HCD spectra	802:827	O-glycopeptide HCD spectra	802:827	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	1	65	from	modification	208:219	arg1	s					245:245	the specific residue(s)	224:246	the specific residue(s)	224:246	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	1	66	theme	modifications	126:138	arg1	analysis					83:90	the analysis	79:90	the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation	79:186	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	9	67	theme	glycopeptide	1498:1509	arg1	identification					1511:1524	glycopeptide identification	1498:1524	glycopeptide identification	1498:1524	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	68	from	%	1436:1436	arg1	spectra					1480:1486	glycopeptide spectra matching	1467:1495	glycopeptide spectra matching	1467:1495	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	68	from	%	1436:1436	arg1	identification					1548:1561	peptide sequence identification	1531:1561	peptide sequence identification	1531:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	9	68	from	%	1436:1436	arg1	identification					1511:1524	glycopeptide identification	1498:1524	glycopeptide identification	1498:1524	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	1	69	theme	residue	237:243	arg1	s					245:245	the specific residue(s)	224:246	the specific residue(s)	224:246	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	8	70	gly	O-glycopeptides	1349:1363	arg2	O-glycopeptides					1349:1363	O-glycopeptides	1349:1363	O-glycopeptides	1349:1363	It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides.					
30802037	5	71	theme	Ser/Thr	879:885	arg1	residues					887:894	the Ser/Thr residues	875:894	the Ser/Thr residues	875:894	Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level.					
30802037	6	72	theme	possible	1008:1015	arg1	mass					1024:1027	every possible summed mass	1002:1027	every possible summed mass of O-glycan combinations on at most three S/T residues	1002:1082	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	7	73	theme	glycan	1210:1215	arg1	modifications					1217:1229	variable glycan modifications	1201:1229	variable glycan modifications	1201:1229	All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications.					
30802037	6	74	theme	MS/MS	971:975	arg1	spectrum					977:984	the MS/MS spectrum	967:984	the MS/MS spectrum	967:984	The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues.					
30802037	0	75	theme	Glycopeptides	60:72	arg1	Identification					33:46	the Identification	29:46	the Identification of O-Linked Glycopeptides	29:72	A New Searching Strategy for the Identification of O-Linked Glycopeptides.					
30802037	2	76	theme	due	410:412	arg1	O-glycopeptides					394:408	intact mucin-type O-glycopeptides	376:408	intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation	376:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	2	77	gly	microheterogeneity	426:443	arg1	glycosylation					452:464	the glycosylation	448:464	the glycosylation	448:464	However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation.					
30802037	9	78	theme	%	1450:1450	arg1	improvement					1452:1462	79% improvement	1448:1462	79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification	1448:1561	Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively.					
30802037	1	79	theme	protein	148:154	arg1	phosphorylation					156:170	protein phosphorylation	148:170	protein phosphorylation	148:170	For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching.					
30802037	0	80	theme	O-Linked	51:58	arg1	Glycopeptides					60:72	O-Linked Glycopeptides	51:72	O-Linked Glycopeptides	51:72	A New Searching Strategy for the Identification of O-Linked Glycopeptides.					
30802037	3	81	theme	peptide	693:699	arg1	sequences					701:709	the peptide sequences	689:709	the peptide sequences	689:709	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	4	82	gly	O-glycopeptide	802:815	arg2	O-glycopeptide					802:815	O-glycopeptide HCD spectra	802:827	O-glycopeptide HCD spectra	802:827	In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra.					
30802037	3	83	gly	O-glycopeptides	557:571	arg2	O-glycopeptides					557:571	the O-glycopeptides	553:571	the O-glycopeptides	553:571	Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences.					
30802037	0	84	theme	New	2:4	arg1	Strategy					16:23	A New Searching Strategy	0:23	A New Searching Strategy for the Identification of O-Linked Glycopeptides	0:72	A New Searching Strategy for the Identification of O-Linked Glycopeptides.					
30513306	4	0	theme	periodic	671:678	arg1	expression					680:689	periodic expression	671:689	periodic expression	671:689	LmAbd-9 was highly expressed in the integument and showed periodic expression during molting.					
30513306	1	1	theme	chitin	142:147	arg1	filaments					149:157	chitin filaments	142:157	chitin filaments embedded in a proteinaceous matrix	142:192	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	0	2	theme	endocuticle	69:79	arg1	formation					52:60	the formation	48:60	the formation of the endocuticle during locust molting	48:101	Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting.					
30513306	7	3	theme	in	1396:1397	arg1	migratoria					1402:1411	the endocuticle in L. migratoria	1380:1411	the endocuticle in L. migratoria	1380:1411	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	3	4	gly	glycoprotein	406:417	arg1	LmAbd-9					388:394	LmAbd-9	388:394	LmAbd-9	388:394	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	4	gly	glycoprotein	406:417	arg1	glycoprotein					406:417	a glycoprotein	404:417	a glycoprotein	404:417	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	5	theme	family	489:494	arg1	subclass					469:476	RR-1 subclass	464:476	RR-1 subclass of the CPR family	464:494	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	7	6	theme	L.	1399:1400	arg1	migratoria					1402:1411	the endocuticle in L. migratoria	1380:1411	the endocuticle in L. migratoria	1380:1411	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	2	7	theme	endocuticle	272:282	arg1	LmAbd-9					314:320	LmAbd-9	314:320	LmAbd-9	314:320	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	2	7	theme	endocuticle	272:282	arg1	gene					308:311	an endocuticle structural glycoprotein gene	269:311	an endocuticle structural glycoprotein gene	269:311	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	2	8	theme	structural	284:293	arg1	LmAbd-9					314:320	LmAbd-9	314:320	LmAbd-9	314:320	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	2	8	theme	structural	284:293	arg1	gene					308:311	an endocuticle structural glycoprotein gene	269:311	an endocuticle structural glycoprotein gene	269:311	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	0	9	theme	locust	88:93	arg1	molting					95:101	locust molting	88:101	locust molting	88:101	Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting.					
30513306	3	10	theme	O-linked	521:528	arg1	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	10	theme	O-linked	521:528	arg1	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	10	theme	O-linked	521:528	arg1	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	11	theme	CPR	485:487	arg1	family					489:494	the CPR family	481:494	the CPR family	481:494	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	6	12	theme	nymphs	1064:1069	arg1	day					1042:1044	day 2	1042:1046	day 2 of fifth instar nymphs	1042:1069	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	7	13	theme	endocuticle	1384:1394	arg1	migratoria					1402:1411	the endocuticle in L. migratoria	1380:1411	the endocuticle in L. migratoria	1380:1411	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	3	14	with	glycoprotein	406:417	arg1	domain					441:446	a chitin binding domain 4	424:448	a chitin binding domain 4	424:448	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	5	15	theme	RNA	903:905	arg1	interference					907:918	double-stranded RNA-mediated RNA interference	874:918	double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta	874:966	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	1	16	theme	embedded	159:166	arg1	filaments					149:157	chitin filaments	142:157	chitin filaments embedded in a proteinaceous matrix	142:192	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	3	17	link	O-linked	521:528	arg1	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	17	link	O-linked	521:528	arg1	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	17	link	O-linked	521:528	arg1	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	5	18	theme	LmFTZ-F1beta	955:966	arg1	interference					907:918	double-stranded RNA-mediated RNA interference	874:918	double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta	874:966	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	0	19	theme	glycoprotein	11:22	arg1	LmAbd-9					24:30	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting.					
30513306	2	20	gly	glycoprotein	295:306	arg1	glycoprotein					295:306	an endocuticle structural glycoprotein gene	269:311	an endocuticle structural glycoprotein gene	269:311	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	6	21	theme	adults	1179:1184	arg1	thinner					1190:1196	thinner	1190:1196	thinner	1190:1196	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	6	21	theme	adults	1179:1184	arg1	cuticle					1164:1170	the cuticle	1160:1170	the cuticle of the adults	1160:1184	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	7	22	theme	signaling	1329:1337	arg1	pathway					1339:1345	the 20E signaling pathway	1321:1345	the 20E signaling pathway	1321:1345	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	2	23	theme	transcriptome	373:385	arg1	migratoria					362:371	the Locusta migratoria transcriptome	350:385	the Locusta migratoria transcriptome	350:385	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	0	24	theme	Structural	0:9	arg1	LmAbd-9					24:30	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting.					
30513306	6	25	from	injection	1016:1024	arg1	day					1042:1044	day 2	1042:1046	day 2 of fifth instar nymphs	1042:1069	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	5	26	dep	receptor	931:938	arg1	LmEcR					945:949	gene LmEcR	940:949	gene LmEcR	940:949	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	1	27	theme	proteinaceous	173:185	arg1	matrix					187:192	a proteinaceous matrix	171:192	a proteinaceous matrix	171:192	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	2	28	theme	glycoprotein	295:306	arg1	LmAbd-9					314:320	LmAbd-9	314:320	LmAbd-9	314:320	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	2	28	theme	glycoprotein	295:306	arg1	gene					308:311	an endocuticle structural glycoprotein gene	269:311	an endocuticle structural glycoprotein gene	269:311	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	5	29	theme	receptor	931:938	arg1	interference					907:918	double-stranded RNA-mediated RNA interference	874:918	double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta	874:966	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	5	30	theme	gene	940:943	arg1	LmEcR					945:949	gene LmEcR	940:949	gene LmEcR	940:949	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	1	31	theme	Insect	104:109	arg1	cuticle					111:117	Insect cuticle	104:117	Insect cuticle	104:117	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	1	31	theme	Insect	104:109	arg1	composite					124:132	a composite	122:132	a composite	122:132	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	6	32	theme	instar	1057:1062	arg1	nymphs					1064:1069	fifth instar nymphs	1051:1069	fifth instar nymphs	1051:1069	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	5	33	theme	fifth	980:984	arg1	nymphs					993:998	fifth instar nymphs	980:998	fifth instar nymphs for 48 h	980:1007	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	3	34	theme	chitin	426:431	arg1	domain					441:446	a chitin binding domain 4	424:448	a chitin binding domain 4	424:448	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	35	theme	binding	433:439	arg1	domain					441:446	a chitin binding domain 4	424:448	a chitin binding domain 4	424:448	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	0	36	gly	glycoprotein	11:22	arg1	glycoprotein					11:22	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9	0:30	Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting.					
30513306	6	37	theme	dsLmAbd-9	1029:1037	arg1	injection					1016:1024	injection	1016:1024	injection of dsLmAbd-9 on day 2 of fifth instar nymphs	1016:1069	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	3	38	contain	has	503:505	arg2	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	38	contain	has	503:505	arg2	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	38	contain	has	503:505	arg1	LmAbd-9					388:394	LmAbd-9	388:394	LmAbd-9	388:394	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	38	contain	has	503:505	arg1	glycoprotein					406:417	a glycoprotein	404:417	a glycoprotein	404:417	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	38	contain	has	503:505	arg2	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	5	39	theme	nymphs	993:998	arg1	day					971:973	day 2	971:975	day 2 of fifth instar nymphs for 48 h	971:1007	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	5	40	theme	double-stranded	874:888	arg1	interference					907:918	double-stranded RNA-mediated RNA interference	874:918	double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta	874:966	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	5	41	theme	20E	927:929	arg1	receptor					931:938	the 20E receptor gene LmEcR and LmFTZ-F1beta	923:966	receptor	931:938	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	6	42	theme	endocuticular	1234:1246	arg1	lamellae					1248:1255	significantly fewer endocuticular lamellae	1214:1255	significantly fewer endocuticular lamellae	1214:1255	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	1	43	from	matrix	187:192	arg1	embedded					159:166	embedded	159:166	embedded	159:166	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	5	44	theme	RNA-mediated	890:901	arg1	interference					907:918	double-stranded RNA-mediated RNA interference	874:918	double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta	874:966	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	6	45	theme	macroscopic	1128:1138	arg1	phenotype					1140:1148	no macroscopic phenotype	1125:1148	no macroscopic phenotype	1125:1148	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	6	46	theme	fewer	1228:1232	arg1	lamellae					1248:1255	significantly fewer endocuticular lamellae	1214:1255	significantly fewer endocuticular lamellae	1214:1255	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	2	47	theme	present	254:260	arg1	study					262:266	the present study	250:266	the present study	250:266	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	5	48	theme	instar	986:991	arg1	nymphs					993:998	fifth instar nymphs	980:998	fifth instar nymphs for 48 h	980:1007	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	3	49	theme	RR-1	464:467	arg1	subclass					469:476	RR-1 subclass	464:476	RR-1 subclass of the CPR family	464:494	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	50	theme	glycosylation	530:542	arg1	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	50	theme	glycosylation	530:542	arg1	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	50	theme	glycosylation	530:542	arg1	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	7	51	theme	20E	1325:1327	arg1	pathway					1339:1345	the 20E signaling pathway	1321:1345	the 20E signaling pathway	1321:1345	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	5	52	with	injection	780:788	arg1	20E					815:817	20E	815:817	20E	815:817	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	5	52	with	injection	780:788	arg1	20-hydroxyecdysone					795:812	20-hydroxyecdysone	795:812	20-hydroxyecdysone (20E)	795:818	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	3	53	theme	potential	511:519	arg1	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	53	theme	potential	511:519	arg1	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	53	theme	potential	511:519	arg1	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	54	gly	glycosylation	530:542	arg2	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	54	gly	glycosylation	530:542	arg2	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	54	gly	glycosylation	530:542	arg2	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	54	gly	glycosylation	530:542	arg2	two					507:509	two	507:509	two	507:509	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	1	55	theme	structural	216:225	arg1	proteins					237:244	structural cuticular proteins	216:244	structural cuticular proteins	216:244	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	5	56	theme	LmAbd-9	732:738	arg1	levels					722:727	The expression levels	707:727	The expression levels of LmAbd-9	707:738	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30513306	6	57	theme	fifth	1051:1055	arg1	nymphs					1064:1069	fifth instar nymphs	1051:1069	fifth instar nymphs	1051:1069	After injection of dsLmAbd-9 on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control.					
30513306	2	58	theme	Locusta	354:360	arg1	migratoria					362:371	the Locusta migratoria transcriptome	350:385	the Locusta migratoria transcriptome	350:385	In the present study, an endocuticle structural glycoprotein gene, LmAbd-9, was characterized based on the Locusta migratoria transcriptome.					
30513306	1	59	theme	cuticular	227:235	arg1	proteins					237:244	structural cuticular proteins	216:244	structural cuticular proteins	216:244	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	7	60	theme	migratoria	1402:1411	arg1	formation					1367:1375	the formation	1363:1375	the formation of the endocuticle in L. migratoria	1363:1411	Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle in L. migratoria.					
30513306	3	61	theme	glycosylation	575:587	arg1	modification					589:600	glycosylation modification	575:600	glycosylation modification	575:600	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	1	62	from	embedded	159:166	arg1	matrix					187:192	a proteinaceous matrix	171:192	a proteinaceous matrix	171:192	Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins.					
30513306	3	63	dep	sites	544:548	arg1	sites					544:548	two potential O-linked glycosylation sites	507:548	two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur	507:610	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	63	dep	sites	544:548	arg1	T137					560:563	T137	560:563	T137	560:563	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	3	63	dep	sites	544:548	arg1	S115					551:554	S115	551:554	S115	551:554	LmAbd-9 encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.					
30513306	5	64	theme	expression	711:720	arg1	levels					722:727	The expression levels	707:727	The expression levels of LmAbd-9	707:738	The expression levels of LmAbd-9 were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor gene LmEcR and LmFTZ-F1beta at day 2 of fifth instar nymphs for 48 h.					
30874698	5	0	theme	enthalpy	1753:1760	arg1	change					1762:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change	1167:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1)	1167:1799	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	0	theme	enthalpy	1753:1760	arg1	g-1					1796:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	1	theme	SPI	611:613	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	1	theme	SPI	611:613	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	1	theme	SPI	611:613	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	1	theme	SPI	611:613	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	1	theme	SPI	611:613	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	2	theme	aqueous	1227:1233	arg1	dispersion					1235:1244	aqueous dispersion	1227:1244	aqueous dispersion	1227:1244	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	3	theme	m2	1505:1506	arg1	protein					1512:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	3	theme	m2	1505:1506	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	1	4	theme	low-water	241:249	arg1	systems					311:317	solid-state and wet-heating reaction systems	274:317	solid-state and wet-heating reaction systems	274:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	1	4	theme	low-water	241:249	arg1	media					260:264	low-water reaction media	241:264	low-water reaction media such as solid-state and wet-heating reaction systems	241:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	5	5	dep	6.69	1333:1336	arg1	stability					1598:1606	reduced thermal stability	1582:1606	reduced thermal stability	1582:1606	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	5	dep	6.69	1333:1336	arg1	%					1714:1714	50.3%	1710:1714	50.3%	1710:1714	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	6	6	theme	greater	1953:1959	arg1	modification					1970:1981	about greater property modification	1947:1981	about greater property modification	1947:1981	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	3	7	theme	single	532:537	arg1	experiments					546:556	serial single factor experiments	525:556	serial single factor experiments	525:556	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	8	from	conditions	730:739	arg1	IL					767:768	this IL	762:768	this IL	762:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	4	9	theme	kg-1	893:896	arg1	protein					898:904	19.88 and 6.26 g kg-1 protein	876:904	19.88 and 6.26 g kg-1 protein	876:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	5	10	theme	-OH	1026:1028	arg1	structure					1059:1067	disordered secondary structure	1038:1067	disordered secondary structure	1038:1067	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	10	theme	-OH	1026:1028	arg1	aggregation					1086:1096	greater protein aggregation	1070:1096	greater protein aggregation	1070:1096	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	10	theme	-OH	1026:1028	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	6.69					1333:1336	6.69	1333:1336	6.69	1333:1336	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	loss					1704:1707	larger total mass loss	1686:1707	larger total mass loss	1686:1707	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	%					1727:1727	57.7%	1723:1727	57.7%	1723:1727	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	11	dep	water-binding	1318:1330	arg1	8.98					1345:1348	8.98	1345:1348	8.98	1345:1348	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	12	theme	1 	629:630	arg1	maltose					618:624	maltose	618:624	maltose of 1 	618:630	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	1	13	theme	Maillard	155:162	arg1	type					164:167	the Maillard type	151:167	the Maillard type	151:167	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	6	14	theme	SPI	1914:1916	arg1	glycation					1918:1926	the SPI glycation	1910:1926	the SPI glycation in this IL	1910:1937	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	5	15	contain	had	1017:1019	arg2	structure					1059:1067	disordered secondary structure	1038:1067	disordered secondary structure	1038:1067	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	15	contain	had	1017:1019	arg1	GSPI-II					1009:1015	GSPI-II	1009:1015	GSPI-II	1009:1015	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	15	contain	had	1017:1019	arg1	GSPI-I					998:1003	GSPI-I	998:1003	GSPI-I	998:1003	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	15	contain	had	1017:1019	arg2	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	15	contain	had	1017:1019	arg2	aggregation					1086:1096	greater protein aggregation	1070:1096	greater protein aggregation	1070:1096	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	16	theme	in	1284:1285	arg1	digestibility					1293:1305	in vitro digestibility	1284:1305	in vitro digestibility	1284:1305	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	17	theme	serial	525:530	arg1	experiments					546:556	serial single factor experiments	525:556	serial single factor experiments	525:556	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	2	18	theme	reaction	498:505	arg1	equilibrium					507:517	the reaction equilibrium	494:517	the reaction equilibrium	494:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	3	19	theme	factor	539:544	arg1	experiments					546:556	serial single factor experiments	525:556	serial single factor experiments	525:556	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	20	theme	kJ	1793:1794	arg1	g-1					1796:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	20	theme	kJ	1793:1794	arg1	change					1762:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change	1167:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1)	1167:1799	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	7	21	theme	product	2118:2124	arg1	properties					2126:2135	product properties	2118:2135	product properties	2118:2135	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	5	22	theme	larger	1686:1691	arg1	6.69					1333:1336	6.69	1333:1336	6.69	1333:1336	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	22	theme	larger	1686:1691	arg1	loss					1704:1707	larger total mass loss	1686:1707	larger total mass loss	1686:1707	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	0	23	theme	Maillard	111:118	arg1	reaction					120:127	the Maillard reaction	107:127	the Maillard reaction	107:127	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	6	24	theme	greater	1835:1841	arg1	change					1843:1848	greater change	1835:1848	greater change	1835:1848	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	5	25	theme	g	1417:1417	arg1	protein					1423:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	25	theme	g	1417:1417	arg1	capacities					1432:1441	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	capacities	1432:1441	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	26	theme	mass	1699:1702	arg1	6.69					1333:1336	6.69	1333:1336	6.69	1333:1336	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	26	theme	mass	1699:1702	arg1	loss					1704:1707	larger total mass loss	1686:1707	larger total mass loss	1686:1707	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	27	theme	thermal	1590:1596	arg1	stability					1598:1606	reduced thermal stability	1582:1606	reduced thermal stability	1582:1606	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	4	28	dep	IL	931:932	arg1	this					926:929	this	926:929	this	926:929	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	6	29	theme	property	1961:1968	arg1	modification					1970:1981	about greater property modification	1947:1981	about greater property modification	1947:1981	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	6	30	from	glycation	1918:1926	arg1	IL					1936:1937	this IL	1931:1937	this IL	1931:1937	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	5	31	theme	hydrodynamic	1110:1121	arg1	radius					1123:1128	larger hydrodynamic radius	1103:1128	larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm)	1103:1161	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	31	theme	hydrodynamic	1110:1121	arg1	nm					1159:1160	76.7 versus 143.8 and 105.1 nm	1131:1160	76.7 versus 143.8 and 105.1 nm	1131:1160	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	32	theme	many	1021:1024	arg1	structure					1059:1067	disordered secondary structure	1038:1067	disordered secondary structure	1038:1067	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	32	theme	many	1021:1024	arg1	aggregation					1086:1096	greater protein aggregation	1070:1096	greater protein aggregation	1070:1096	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	32	theme	many	1021:1024	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	33	theme	SPI	562:564	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	33	theme	SPI	562:564	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	33	theme	SPI	562:564	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	33	theme	SPI	562:564	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	33	theme	SPI	562:564	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	1	34	theme	Protein	130:136	arg1	glycation					138:146	Protein glycation	130:146	Protein glycation of the Maillard type	130:167	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	3	35	theme	SPI	745:747	arg1	glycation					749:757	SPI glycation	745:757	SPI glycation	745:757	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	36	theme	57.7	1723:1726	arg1	%					1727:1727	57.7%	1723:1727	57.7%	1723:1727	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	37	theme	protein	1078:1084	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	37	theme	protein	1078:1084	arg1	aggregation					1086:1096	greater protein aggregation	1070:1096	greater protein aggregation	1070:1096	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	2	38	theme	water	466:470	arg1	activity					472:479	water activity	466:479	water activity	466:479	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	5	39	theme	surface	1257:1263	arg1	hydrophobicity					1265:1278	decreased surface hydrophobicity	1247:1278	decreased surface hydrophobicity	1247:1278	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	2	40	theme	reaction	383:390	arg1	liquid					329:334	liquid	329:334	liquid	329:334	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	40	theme	reaction	383:390	arg1	medium					392:397	a promising reaction medium	371:397	a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium	371:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	5	41	dep	Compared	959:966	arg1	both					993:996	both	993:996	both	993:996	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	0	42	theme	property	14:21	arg1	changes					23:29	property changes	14:29	property changes	14:29	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	1	43	theme	solid-state	274:284	arg1	systems					311:317	solid-state and wet-heating reaction systems	274:317	solid-state and wet-heating reaction systems	274:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	2	44	theme	isolate	440:446	arg1	glycation					415:423	the maltose glycation	403:423	the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium	403:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	0	45	theme	isolate	50:56	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	0	45	theme	isolate	50:56	arg1	changes					23:29	property changes	14:29	property changes	14:29	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	5	46	theme	8.21	1354:1357	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	46	theme	8.21	1354:1357	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	46	theme	8.21	1354:1357	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	47	theme	emulsifying	1451:1461	arg1	protein					1512:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	47	theme	emulsifying	1451:1461	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	47	theme	emulsifying	1451:1461	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	2	48	theme	promising	373:381	arg1	liquid					329:334	liquid	329:334	liquid	329:334	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	48	theme	promising	373:381	arg1	medium					392:397	a promising reaction medium	371:397	a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium	371:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	1	49	theme	wet-heating	290:300	arg1	systems					311:317	solid-state and wet-heating reaction systems	274:317	solid-state and wet-heating reaction systems	274:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	4	50	with	GSPI-I	832:837	arg1	contents					864:871	maltose contents	856:871	maltose contents of 19.88 and 6.26 g kg-1 protein	856:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	51	theme	maltose	856:862	arg1	contents					864:871	maltose contents	856:871	maltose contents of 19.88 and 6.26 g kg-1 protein	856:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	1	52	theme	reaction	251:258	arg1	systems					311:317	solid-state and wet-heating reaction systems	274:317	solid-state and wet-heating reaction systems	274:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	1	52	theme	reaction	251:258	arg1	media					260:264	low-water reaction media	241:264	low-water reaction media such as solid-state and wet-heating reaction systems	241:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	3	53	theme	g	587:587	arg1	kg-1					589:592	150 g kg-1	583:592	150 g kg-1	583:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	54	theme	g-1	1508:1510	arg1	protein					1512:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	54	theme	g-1	1508:1510	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	0	55	theme	soy	38:40	arg1	isolate					50:56	the soy protein isolate	34:56	the soy protein isolate	34:56	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	5	56	theme	unglycated	977:986	arg1	SPI					988:990	the unglycated SPI	973:990	the unglycated SPI	973:990	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	1	57	theme	protein	203:209	arg1	functionalities					211:225	protein functionalities	203:225	protein functionalities	203:225	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	5	58	theme	oil-binding	1378:1388	arg1	protein					1423:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	58	theme	oil-binding	1378:1388	arg1	capacities					1432:1441	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	capacities	1432:1441	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	59	theme	decomposition	1619:1631	arg1	temperature					1633:1643	lower decomposition temperature	1613:1643	lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C)	1613:1683	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	60	theme	disordered	1038:1047	arg1	structure					1059:1067	disordered secondary structure	1038:1067	disordered secondary structure	1038:1067	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	60	theme	disordered	1038:1047	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	2	61	theme	soy	428:430	arg1	SPI					449:451	SPI	449:451	SPI	449:451	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	61	theme	soy	428:430	arg1	isolate					440:446	soy protein isolate	428:446	soy protein isolate (SPI)	428:452	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	5	62	theme	smaller	1745:1751	arg1	change					1762:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change	1167:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1)	1167:1799	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	62	theme	smaller	1745:1751	arg1	g-1					1796:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	7	63	theme	interesting	2034:2044	arg1	medium					2046:2051	an interesting medium	2031:2051	an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently	2031:2147	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	7	63	theme	interesting	2034:2044	arg1	IL					2008:2009	This IL	2003:2009	This IL	2003:2009	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	5	64	theme	zeta-potential	1176:1189	arg1	change					1762:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change	1167:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1)	1167:1799	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	64	theme	zeta-potential	1176:1189	arg1	g-1					1796:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	4	65	theme	protein	898:904	arg1	contents					864:871	maltose contents	856:871	maltose contents of 19.88 and 6.26 g kg-1 protein	856:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	5	66	theme	g-1	1361:1363	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	66	theme	g-1	1361:1363	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	66	theme	g-1	1361:1363	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	67	dep	ratio	602:606	arg1	 1					632:633	 1	632:633	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	68	theme	decreased	1247:1255	arg1	hydrophobicity					1265:1278	decreased surface hydrophobicity	1247:1278	decreased surface hydrophobicity	1247:1278	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	69	theme	g-1	1419:1421	arg1	protein					1423:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	2.80 versus 3.11 and 3.09 g g-1 protein	1391:1429	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	69	theme	g-1	1419:1421	arg1	capacities					1432:1441	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	capacities	1432:1441	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	70	theme	emulsion	1525:1532	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	70	theme	emulsion	1525:1532	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	1	71	theme	type	164:167	arg1	glycation					138:146	Protein glycation	130:146	Protein glycation of the Maillard type	130:167	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	4	72	with	GSPI-II	843:849	arg1	contents					864:871	maltose contents	856:871	maltose contents of 19.88 and 6.26 g kg-1 protein	856:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	5	73	theme	stability	1534:1542	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	73	theme	stability	1534:1542	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	74	theme	reaction	638:645	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	74	theme	reaction	638:645	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	74	theme	reaction	638:645	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	74	theme	reaction	638:645	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	74	theme	reaction	638:645	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	75	dep	in	1284:1285	arg1	vitro					1287:1291	vitro	1287:1291	vitro	1287:1291	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	76	theme	total	1693:1697	arg1	6.69					1333:1336	6.69	1333:1336	6.69	1333:1336	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	76	theme	total	1693:1697	arg1	loss					1704:1707	larger total mass loss	1686:1707	larger total mass loss	1686:1707	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	77	theme	reduced	1582:1588	arg1	stability					1598:1606	reduced thermal stability	1582:1606	reduced thermal stability	1582:1606	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	78	theme	°C	666:667	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	78	theme	°C	666:667	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	78	theme	°C	666:667	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	78	theme	°C	666:667	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	78	theme	°C	666:667	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	79	theme	suitable	721:728	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	79	theme	suitable	721:728	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	79	theme	suitable	721:728	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	79	theme	suitable	721:728	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	79	theme	suitable	721:728	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	80	theme	larger	1103:1108	arg1	radius					1123:1128	larger hydrodynamic radius	1103:1128	larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm)	1103:1161	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	80	theme	larger	1103:1108	arg1	nm					1159:1160	76.7 versus 143.8 and 105.1 nm	1131:1160	76.7 versus 143.8 and 105.1 nm	1131:1160	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	81	dep	temperature	1633:1643	arg1	°C					1668:1669	297.1 °C versus 289.1 °C and 292.9 °C	1646:1682	°C	1668:1669	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	81	dep	temperature	1633:1643	arg1	°C					1681:1682	297.1 °C versus 289.1 °C and 292.9 °C	1646:1682	°C	1681:1682	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	81	dep	temperature	1633:1643	arg1	°C					1652:1653	297.1 °C versus 289.1 °C and 292.9 °C	1646:1682	°C	1652:1653	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	82	theme	reaction	676:683	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	82	theme	reaction	676:683	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	82	theme	reaction	676:683	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	82	theme	reaction	676:683	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	82	theme	reaction	676:683	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	83	theme	greater	1070:1076	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	83	theme	greater	1070:1076	arg1	aggregation					1086:1096	greater protein aggregation	1070:1096	greater protein aggregation	1070:1096	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	3	84	theme	h	695:695	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	84	theme	h	695:695	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	84	theme	h	695:695	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	84	theme	h	695:695	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	84	theme	h	695:695	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	4	85	theme	glycated	802:809	arg1	GSPI-II					843:849	GSPI-II	843:849	GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein	843:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	85	theme	glycated	802:809	arg1	products					815:822	two glycated SPI products	798:822	two glycated SPI products	798:822	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	85	theme	glycated	802:809	arg1	GSPI-I					832:837	GSPI-I	832:837	GSPI-I	832:837	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	86	theme	SPI	811:813	arg1	GSPI-II					843:849	GSPI-II	843:849	GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein	843:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	86	theme	SPI	811:813	arg1	products					815:822	two glycated SPI products	798:822	two glycated SPI products	798:822	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	4	86	theme	SPI	811:813	arg1	GSPI-I					832:837	GSPI-I	832:837	GSPI-I	832:837	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	2	87	dep	glycation	415:423	arg1	shift					488:492	shift	488:492	to shift the reaction equilibrium	485:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	87	dep	glycation	415:423	arg1	decrease					457:464	decrease	457:464	to decrease water activity	454:479	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	88	theme	maltose	407:413	arg1	glycation					415:423	the maltose glycation	403:423	the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium	403:517	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	3	89	theme	mass	597:600	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	89	theme	mass	597:600	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	89	theme	mass	597:600	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	89	theme	mass	597:600	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	89	theme	mass	597:600	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	90	dep	zeta-potential	1176:1189	arg1	mV					1220:1221	-19.8 versus-20.6 and -20.3 mV	1192:1221	-19.8 versus-20.6 and -20.3 mV	1192:1221	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	90	dep	zeta-potential	1176:1189	arg1	increased					1308:1316	increased	1308:1316	increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%)	1308:1738	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	7	91	theme	glycation	2087:2095	arg1	extent					2097:2102	glycation extent	2087:2102	glycation extent	2087:2102	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	3	92	theme	kg-1	589:592	arg1	temperature					647:657	a reaction temperature	636:657	a reaction temperature of 100 °C	636:667	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	92	theme	kg-1	589:592	arg1	time					685:688	a reaction time	674:688	a reaction time of 2 h	674:695	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	92	theme	kg-1	589:592	arg1	ratio					602:606	a mass ratio	595:606	a mass ratio of SPI to maltose of 1 : 1	595:633	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	92	theme	kg-1	589:592	arg1	concentration					566:578	an SPI concentration	559:578	an SPI concentration of 150 g kg-1	559:592	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	3	92	theme	kg-1	589:592	arg1	conditions					730:739	the suitable conditions	717:739	the suitable conditions for SPI glycation in this IL	717:768	With serial single factor experiments, an SPI concentration of 150 g kg-1, a mass ratio of SPI to maltose of 1 : 1, a reaction temperature of 100 °C, and a reaction time of 2 h were verified to be the suitable conditions for SPI glycation in this IL.					
30874698	5	93	theme	higher	1444:1449	arg1	protein					1512:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	93	theme	higher	1444:1449	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	93	theme	higher	1444:1449	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	2	94	theme	protein	432:438	arg1	SPI					449:451	SPI	449:451	SPI	449:451	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	2	94	theme	protein	432:438	arg1	isolate					440:446	soy protein isolate	428:446	soy protein isolate (SPI)	428:452	An ionic liquid (IL) was explored in this study as a promising reaction medium for the maltose glycation of soy protein isolate (SPI) to decrease water activity and to shift the reaction equilibrium.					
30874698	5	95	theme	g	1359:1359	arg1	index					1544:1548	emulsion stability index	1525:1548	emulsion stability index (58.4% versus 86.8% and 69.3%)	1525:1579	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	95	theme	g	1359:1359	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	95	theme	g	1359:1359	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	7	96	dep	medium	2046:2051	arg1	change					2111:2116	change	2111:2116	to change product properties efficiently	2108:2147	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	7	96	dep	medium	2046:2051	arg1	increase					2078:2085	increase	2078:2085	to increase glycation extent	2075:2102	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	5	97	theme	activity	1463:1470	arg1	protein					1512:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	32.4 versus 56.5 and 40.9 m2 g-1 protein	1479:1518	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	97	theme	activity	1463:1470	arg1	protein					1365:1371	8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities	1354:1441	protein	1365:1371	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	97	theme	activity	1463:1470	arg1	index					1472:1476	higher emulsifying activity index	1444:1476	higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein)	1444:1519	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	0	98	theme	protein	42:48	arg1	isolate					50:56	the soy protein isolate	34:56	the soy protein isolate	34:56	Structure and property changes of the soy protein isolate glycated with maltose in an ionic liquid through the Maillard reaction.					
30874698	1	99	theme	reaction	302:309	arg1	systems					311:317	solid-state and wet-heating reaction systems	274:317	solid-state and wet-heating reaction systems	274:317	Protein glycation of the Maillard type is successfully applied to modify protein functionalities, mainly using low-water reaction media such as solid-state and wet-heating reaction systems.					
30874698	7	100	theme	protein	2057:2063	arg1	glycation					2065:2073	protein glycation	2057:2073	protein glycation	2057:2073	This IL is thus verified as an interesting medium for protein glycation to increase glycation extent and to change product properties efficiently.					
30874698	5	101	theme	lower	1613:1617	arg1	temperature					1633:1643	lower decomposition temperature	1613:1643	lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C)	1613:1683	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	6	102	theme	important	1859:1867	arg1	properties					1869:1878	these important properties	1853:1878	these important properties than GSPI-II	1853:1891	In general, GSPI-I always showed greater change in these important properties than GSPI-II, suggesting that the SPI glycation in this IL brought about greater property modification than that in water.					
30874698	4	103	theme	g	891:891	arg1	protein					898:904	19.88 and 6.26 g kg-1 protein	876:904	19.88 and 6.26 g kg-1 protein	876:904	Using the four conditions, two glycated SPI products, namely GSPI-I and GSPI-II with maltose contents of 19.88 and 6.26 g kg-1 protein, were prepared with this IL and water, respectively.					
30874698	5	104	dep	index	1544:1548	arg1	%					1555:1555	58.4% versus 86.8% and 69.3%	1551:1578	%	1555:1555	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	104	dep	index	1544:1548	arg1	%					1568:1568	58.4% versus 86.8% and 69.3%	1551:1578	%	1568:1568	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	104	dep	index	1544:1548	arg1	%					1578:1578	58.4% versus 86.8% and 69.3%	1551:1578	%	1578:1578	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	105	theme	negative	1167:1174	arg1	change					1762:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change	1167:1767	negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1)	1167:1799	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	105	theme	negative	1167:1174	arg1	g-1					1796:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	9.9 versus 4.4 and 7.2 kJ g-1	1770:1798	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	106	theme	secondary	1049:1057	arg1	structure					1059:1067	disordered secondary structure	1038:1067	disordered secondary structure	1038:1067	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30874698	5	106	theme	secondary	1049:1057	arg1	groups					1030:1035	many -OH groups	1021:1035	many -OH groups	1021:1035	Compared with the unglycated SPI, both GSPI-I and GSPI-II had many -OH groups, disordered secondary structure, greater protein aggregation with larger hydrodynamic radius (76.7 versus 143.8 and 105.1 nm) and negative zeta-potential (-19.8 versus-20.6 and -20.3 mV) in aqueous dispersion, decreased surface hydrophobicity and in vitro digestibility, increased water-binding (6.69 versus 8.98 and 8.21 g g-1 protein) and oil-binding (2.80 versus 3.11 and 3.09 g g-1 protein) capacities, higher emulsifying activity index (32.4 versus 56.5 and 40.9 m2 g-1 protein) and emulsion stability index (58.4% versus 86.8% and 69.3%), reduced thermal stability with lower decomposition temperature (297.1 °C versus 289.1 °C and 292.9 °C), larger total mass loss (50.3% versus 57.7% and 51.3%), and smaller enthalpy change (9.9 versus 4.4 and 7.2 kJ g-1).					
30305426	9	0	from	sugars	1504:1509	arg1	proteins					1527:1534	cell-surface proteins	1514:1534	cell-surface proteins	1514:1534	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	9	0	from	sugars	1504:1509	arg1	proteins					1542:1549	proteins	1542:1549	proteins secreted from an XBP1s-activated cell	1542:1587	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	7	1	theme	maturation	1292:1301	arg1	pathways					1303:1310	N-glycan maturation pathways	1283:1310	N-glycan maturation pathways	1283:1310	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	9	2	theme	stress	1730:1735	arg1	signaling					1737:1745	intracellular stress signaling	1716:1745	intracellular stress signaling	1716:1745	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	4	3	theme	XBP1s	589:593	arg1	activation					595:604	XBP1s activation	589:604	XBP1s activation	589:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	2	4	theme	-mediated	263:271	arg1	response					273:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	4	theme	-mediated	263:271	arg1	UPR					200:202	the UPR	196:202	the UPR	196:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	4	theme	-mediated	263:271	arg1	arm					189:191	The most ancient arm	172:191	The most ancient arm of the UPR	172:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	0	5	theme	XBP1s	0:4	arg1	activation					6:15	XBP1s activation	0:15	XBP1s activation	0:15	XBP1s activation can globally remodel N-glycan structure distribution patterns.					
30305426	4	6	theme	structures	669:678	arg1	distribution					644:655	the distribution	640:655	the distribution of N-glycan structures on endogenous membrane and secreted proteomes	640:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	9	7	theme	cell-surface	1514:1525	arg1	proteins					1527:1534	cell-surface proteins	1514:1534	cell-surface proteins	1514:1534	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	6	8	theme	XBP1s	993:997	arg1	activation					999:1008	stress-independent XBP1s activation	974:1008	stress-independent XBP1s activation	974:1008	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	9	9	theme	unactivated	1634:1644	arg1	cell					1646:1649	an unactivated cell	1631:1649	an unactivated cell	1631:1649	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	7	10	theme	mRNA	1118:1121	arg1	experiments					1133:1143	mRNA profiling experiments	1118:1143	mRNA profiling experiments	1118:1143	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	4	11	from	shift	631:635	arg1	membrane					694:701	endogenous membrane	683:701	endogenous membrane	683:701	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	11	from	shift	631:635	arg1	proteomes					716:724	secreted proteomes	707:724	secreted proteomes	707:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	11	from	shift	631:635	arg1	distribution					644:655	the distribution	640:655	the distribution of N-glycan structures on endogenous membrane and secreted proteomes	640:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	12	theme	secreted	707:714	arg1	proteomes					716:724	secreted proteomes	707:724	secreted proteomes	707:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	7	13	from	consequence	1225:1235	arg1	part					1217:1220	part	1217:1220	part	1217:1220	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	2	14	theme	spliced	224:230	arg1	protein					246:252	the IRE1-activated spliced X-box binding protein 1	205:254	the IRE1-activated spliced X-box binding protein 1 (XBP1s)	205:262	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	14	theme	spliced	224:230	arg1	XBP1s					257:261	XBP1s	257:261	XBP1s	257:261	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	6	15	theme	cell	958:961	arg1	membranes					963:971	HeLa cell membranes	953:971	HeLa cell membranes	953:971	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	8	16	theme	N-glycan	1353:1360	arg1	remodeling					1373:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	8	17	theme	remodeling	1373:1382	arg1	discovery					1326:1334	The discovery	1322:1334	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale	1322:1406	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	9	18	theme	XBP1s-activated	1568:1582	arg1	cell					1584:1587	an XBP1s-activated cell	1565:1587	an XBP1s-activated cell	1565:1587	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	6	19	theme	tetraantennary	1055:1068	arg1	population					1024:1033	the population	1020:1033	the population of high-mannose and tetraantennary N-glycans	1020:1078	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	4	20	theme	endogenous	683:692	arg1	membrane					694:701	endogenous membrane	683:701	endogenous membrane	683:701	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	7	21	theme	N-glycan	1283:1290	arg1	pathways					1303:1310	N-glycan maturation pathways	1283:1310	N-glycan maturation pathways	1283:1310	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	5	22	from	GlcNAc	801:806	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	2	23	theme	binding	238:244	arg1	protein					246:252	the IRE1-activated spliced X-box binding protein 1	205:254	the IRE1-activated spliced X-box binding protein 1 (XBP1s)	205:262	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	23	theme	binding	238:244	arg1	XBP1s					257:261	XBP1s	257:261	XBP1s	257:261	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	5	24	theme	oligomannose	904:915	arg1	N-glycans					917:925	oligomannose N-glycans	904:925	oligomannose N-glycans	904:925	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	9	25	from	those	1622:1626	arg1	distinct					1608:1615	distinct	1608:1615	distinct	1608:1615	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	9	25	from	those	1622:1626	arg1	sugars					1504:1509	the sugars	1500:1509	the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell	1500:1587	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	6	26	theme	high-mannose	1038:1049	arg1	population					1024:1033	the population	1020:1033	the population of high-mannose and tetraantennary N-glycans	1020:1078	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	5	27	theme	sialylation	775:785	arg1	levels					765:770	levels	765:770	levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome	765:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	27	theme	sialylation	775:785	arg1	secretome					844:852	secretome	844:852	secretome	844:852	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	7	28	theme	coordinated	1240:1250	arg1	resculpting					1268:1278	coordinated transcriptional resculpting	1240:1278	coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1240:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	0	29	theme	distribution	57:68	arg1	patterns					70:77	N-glycan structure distribution patterns	38:77	N-glycan structure distribution patterns	38:77	XBP1s activation can globally remodel N-glycan structure distribution patterns.					
30305426	6	30	dep	high-mannose	1038:1049	arg1	N-glycans					1070:1078	N-glycans	1070:1078	N-glycans	1070:1078	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	5	31	gly	sialylation	775:785	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	32	theme	HEK293	815:820	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	1	33	theme	unfolded	97:104	arg1	UPR					124:126	UPR	124:126	UPR	124:126	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	1	33	theme	unfolded	97:104	arg1	response					114:121	the unfolded protein response	93:121	the unfolded protein response (UPR)	93:127	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	6	34	theme	core	1099:1102	arg1	fucosylation					1104:1115	core fucosylation	1099:1115	core fucosylation	1099:1115	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	8	35	theme	master	1441:1446	arg1	XBP1s					1422:1426	XBP1s	1422:1426	XBP1s	1422:1426	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	8	35	theme	master	1441:1446	arg1	regulator					1448:1456	a master regulator	1439:1456	a master regulator of N-glycan maturation	1439:1479	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	2	36	theme	ancient	181:187	arg1	response					273:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	36	theme	ancient	181:187	arg1	arm					189:191	The most ancient arm	172:191	The most ancient arm of the UPR	172:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	36	theme	ancient	181:187	arg1	UPR					200:202	the UPR	196:202	the UPR	196:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	5	37	theme	bisecting	791:799	arg1	GlcNAc					801:806	bisecting GlcNAc	791:806	bisecting GlcNAc	791:806	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	3	38	theme	XBP1s	397:401	arg1	activation					403:412	XBP1s activation	397:412	XBP1s activation	397:412	Understanding the consequences of XBP1s activation for cellular processes is critical for elucidating mechanistic connections between XBP1s and development, immunity, and disease.					
30305426	8	39	theme	maturation	1470:1479	arg1	XBP1s					1422:1426	XBP1s	1422:1426	XBP1s	1422:1426	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	8	39	theme	maturation	1470:1479	arg1	regulator					1448:1456	a master regulator	1439:1456	a master regulator of N-glycan maturation	1439:1479	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	9	40	with	interactions	1760:1771	arg1	environment					1796:1806	the extracellular environment	1778:1806	the extracellular environment	1778:1806	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	0	41	theme	structure	47:55	arg1	patterns					70:77	N-glycan structure distribution patterns	38:77	N-glycan structure distribution patterns	38:77	XBP1s activation can globally remodel N-glycan structure distribution patterns.					
30305426	2	42	theme	UPR	200:202	arg1	response					273:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	42	theme	UPR	200:202	arg1	arm					189:191	The most ancient arm	172:191	The most ancient arm of the UPR	172:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	42	theme	UPR	200:202	arg1	UPR					200:202	the UPR	196:202	the UPR	196:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	43	theme	pathway	306:312	arg1	maturation					314:323	secretory pathway maturation	296:323	secretory pathway maturation	296:323	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	9	44	theme	new	1686:1688	arg1	mechanism					1690:1698	a potential new mechanism	1674:1698	a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment	1674:1806	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	1	45	theme	pathway	150:156	arg1	proteostasis					158:169	secretory pathway proteostasis	140:169	secretory pathway proteostasis	140:169	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	4	46	theme	activation	595:604	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	46	theme	activation	595:604	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	7	47	theme	N-glycome	1191:1199	arg1	remodeling					1173:1182	XBP1s-mediated remodeling	1158:1182	XBP1s-mediated remodeling of the N-glycome	1158:1199	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	7	47	theme	N-glycome	1191:1199	arg1	consequence					1225:1235	a consequence	1223:1235	a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1223:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	2	48	theme	proteostatic	342:353	arg1	stress					355:360	proteostatic stress	342:360	proteostatic stress	342:360	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	4	49	from	membrane	694:701	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	49	from	membrane	694:701	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	9	50	theme	intracellular	1716:1728	arg1	signaling					1737:1745	intracellular stress signaling	1716:1745	intracellular stress signaling	1716:1745	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	7	51	theme	pathways	1303:1310	arg1	resculpting					1268:1278	coordinated transcriptional resculpting	1240:1278	coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1240:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	4	52	theme	functional	568:577	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	52	theme	functional	568:577	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	7	53	theme	XBP1s-mediated	1158:1171	arg1	remodeling					1173:1182	XBP1s-mediated remodeling	1158:1182	XBP1s-mediated remodeling of the N-glycome	1158:1199	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	7	53	theme	XBP1s-mediated	1158:1171	arg1	consequence					1225:1235	a consequence	1223:1235	a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1223:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	9	54	theme	altered	1752:1758	arg1	interactions					1760:1771	altered interactions	1752:1771	altered interactions with the extracellular environment	1752:1806	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	7	55	theme	resculpting	1268:1278	arg1	remodeling					1173:1182	XBP1s-mediated remodeling	1158:1182	XBP1s-mediated remodeling of the N-glycome	1158:1199	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	7	55	theme	resculpting	1268:1278	arg1	consequence					1225:1235	a consequence	1223:1235	a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1223:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	7	56	theme	profiling	1123:1131	arg1	experiments					1133:1143	mRNA profiling experiments	1118:1143	mRNA profiling experiments	1118:1143	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	4	57	from	distribution	644:655	arg1	membrane					694:701	endogenous membrane	683:701	endogenous membrane	683:701	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	57	from	distribution	644:655	arg1	proteomes					716:724	secreted proteomes	707:724	secreted proteomes	707:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	58	theme	N-glycan	660:667	arg1	structures					669:678	N-glycan structures	660:678	N-glycan structures	660:678	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	6	59	theme	stress-independent	974:991	arg1	activation					999:1008	stress-independent XBP1s activation	974:1008	stress-independent XBP1s activation	974:1008	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	9	60	theme	extracellular	1782:1794	arg1	environment					1796:1806	the extracellular environment	1778:1806	the extracellular environment	1778:1806	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	4	61	theme	type-dependent	616:629	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	61	theme	type-dependent	616:629	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	2	62	theme	X-box	232:236	arg1	protein					246:252	the IRE1-activated spliced X-box binding protein 1	205:254	the IRE1-activated spliced X-box binding protein 1 (XBP1s)	205:262	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	62	theme	X-box	232:236	arg1	XBP1s					257:261	XBP1s	257:261	XBP1s	257:261	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	63	theme	IRE1-activated	209:222	arg1	protein					246:252	the IRE1-activated spliced X-box binding protein 1	205:254	the IRE1-activated spliced X-box binding protein 1 (XBP1s)	205:262	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	63	theme	IRE1-activated	209:222	arg1	XBP1s					257:261	XBP1s	257:261	XBP1s	257:261	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	6	64	theme	HeLa	953:956	arg1	membranes					963:971	HeLa cell membranes	953:971	HeLa cell membranes	953:971	In HeLa cell membranes, stress-independent XBP1s activation increased the population of high-mannose and tetraantennary N-glycans, and also enhanced core fucosylation.					
30305426	8	65	theme	XBP1s-induced	1339:1351	arg1	remodeling					1373:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	5	66	from	sialylation	775:785	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	67	from	secretome	844:852	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	8	68	theme	structural	1362:1371	arg1	remodeling					1373:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	XBP1s-induced N-glycan structural remodeling	1339:1382	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	5	69	from	levels	765:770	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	8	70	from	discovery	1326:1334	arg1	scale					1402:1406	a glycome-wide scale	1387:1406	a glycome-wide scale	1387:1406	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	1	71	theme	secretory	140:148	arg1	proteostasis					158:169	secretory pathway proteostasis	140:169	secretory pathway proteostasis	140:169	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	7	72	theme	transcriptional	1252:1266	arg1	resculpting					1268:1278	coordinated transcriptional resculpting	1240:1278	coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s	1240:1319	mRNA profiling experiments suggest that XBP1s-mediated remodeling of the N-glycome is, at least in part, a consequence of coordinated transcriptional resculpting of N-glycan maturation pathways by XBP1s.					
30305426	5	73	theme	N-glycans	917:925	arg1	population					890:899	the population	886:899	the population of oligomannose N-glycans	886:925	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	8	74	theme	glycome-wide	1389:1400	arg1	scale					1402:1406	a glycome-wide scale	1387:1406	a glycome-wide scale	1387:1406	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	2	75	from	roles	287:291	arg1	maturation					314:323	secretory pathway maturation	296:323	secretory pathway maturation	296:323	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	5	76	theme	XBP1s	740:744	arg1	activity					746:753	XBP1s activity	740:753	XBP1s activity	740:753	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	77	from	proteome	831:838	arg1	levels					765:770	levels	765:770	levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome	765:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	77	from	proteome	831:838	arg1	secretome					844:852	secretome	844:852	secretome	844:852	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	78	theme	membrane	822:829	arg1	proteome					831:838	the HEK293 membrane proteome	811:838	the HEK293 membrane proteome	811:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	1	79	theme	protein	106:112	arg1	UPR					124:126	UPR	124:126	UPR	124:126	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	1	79	theme	protein	106:112	arg1	response					114:121	the unfolded protein response	93:121	the unfolded protein response (UPR)	93:127	Classically, the unfolded protein response (UPR) safeguards secretory pathway proteostasis.					
30305426	4	80	theme	key	564:566	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	80	theme	key	564:566	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	0	81	theme	N-glycan	38:45	arg1	patterns					70:77	N-glycan structure distribution patterns	38:77	N-glycan structure distribution patterns	38:77	XBP1s activation can globally remodel N-glycan structure distribution patterns.					
30305426	3	82	theme	cellular	418:425	arg1	processes					427:435	cellular processes	418:435	cellular processes	418:435	Understanding the consequences of XBP1s activation for cellular processes is critical for elucidating mechanistic connections between XBP1s and development, immunity, and disease.					
30305426	3	83	theme	mechanistic	465:475	arg1	connections					477:487	mechanistic connections	465:487	mechanistic connections between XBP1s and development, immunity, and disease	465:540	Understanding the consequences of XBP1s activation for cellular processes is critical for elucidating mechanistic connections between XBP1s and development, immunity, and disease.					
30305426	5	84	theme	GlcNAc	801:806	arg1	levels					765:770	levels	765:770	levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome	765:838	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	5	84	theme	GlcNAc	801:806	arg1	secretome					844:852	secretome	844:852	secretome	844:852	For example, XBP1s activity decreased levels of sialylation and bisecting GlcNAc in the HEK293 membrane proteome and secretome, while substantially increasing the population of oligomannose N-glycans only in the secretome.					
30305426	2	85	contain	has	283:285	arg1	UPR					200:202	the UPR	196:202	the UPR	196:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	85	contain	has	283:285	arg1	response					273:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response	205:280	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	85	contain	has	283:285	arg1	arm					189:191	The most ancient arm	172:191	The most ancient arm of the UPR	172:202	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	2	85	contain	has	283:285	arg2	roles					287:291	roles	287:291	roles in secretory pathway maturation	287:323	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	3	86	theme	activation	403:412	arg1	consequences					381:392	the consequences	377:392	the consequences of XBP1s activation for cellular processes	377:435	Understanding the consequences of XBP1s activation for cellular processes is critical for elucidating mechanistic connections between XBP1s and development, immunity, and disease.					
30305426	8	87	theme	N-glycan	1461:1468	arg1	maturation					1470:1479	N-glycan maturation	1461:1479	N-glycan maturation	1461:1479	The discovery of XBP1s-induced N-glycan structural remodeling on a glycome-wide scale suggests that XBP1s can act as a master regulator of N-glycan maturation.					
30305426	9	88	theme	potential	1676:1684	arg1	mechanism					1690:1698	a potential new mechanism	1674:1698	a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment	1674:1806	Moreover, because the sugars on cell-surface proteins or on proteins secreted from an XBP1s-activated cell can be molecularly distinct from those of an unactivated cell, these findings reveal a potential new mechanism for translating intracellular stress signaling into altered interactions with the extracellular environment.					
30305426	2	89	theme	secretory	296:304	arg1	maturation					314:323	secretory pathway maturation	296:323	secretory pathway maturation	296:323	The most ancient arm of the UPR, the IRE1-activated spliced X-box binding protein 1 (XBP1s)-mediated response, has roles in secretory pathway maturation beyond resolving proteostatic stress.					
30305426	4	90	from	proteomes	716:724	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	90	from	proteomes	716:724	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	91	theme	cell	611:614	arg1	shift					631:635	a cell type-dependent shift	609:635	a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes	609:724	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
30305426	4	91	theme	cell	611:614	arg1	output					579:584	a key functional output	562:584	a key functional output of XBP1s activation	562:604	Here, we show that a key functional output of XBP1s activation is a cell type-dependent shift in the distribution of N-glycan structures on endogenous membrane and secreted proteomes.					
31881512	6	0	theme	optimized	1085:1093	arg1	method					1095:1100	the optimized method	1081:1100	the optimized method	1081:1100	The reproducibility of the optimized method was <3.5%.					
31881512	7	1	theme	Sixty-one	1113:1121	arg1	structures					1132:1141	Sixty-one N-glycan structures	1113:1141	Sixty-one N-glycan structures	1113:1141	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	1	2	theme	great	222:226	arg1	challenge					228:236	a great challenge	220:236	a great challenge to distinguish them based on symptoms only	220:279	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	9	3	theme	peak	1624:1627	arg1	areas					1629:1633	relative peak areas	1615:1633	relative peak areas	1615:1633	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	4	4	theme	human	552:556	arg1	samples					564:570	pooled human serum samples	545:570	pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	545:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	5	with	LC	631:632	arg1	COPD					639:642	COPD	639:642	COPD	639:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	7	6	with	cancer	1244:1249	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	4	7	theme	laser-induced	774:786	arg1	detection					801:809	high sensitivity laser-induced fluorescence detection	757:809	high sensitivity laser-induced fluorescence detection	757:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	8	theme	cancer	601:606	arg1	LC					631:632	LC	631:632	LC with COPD	631:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	8	theme	cancer	601:606	arg1	patients					645:652	lung cancer, COPD and comorbidity (LC with COPD) patients	596:652	patients	645:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	7	9	with	COPD	1252:1255	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	7	10	theme	control	1173:1179	arg1	sample					1193:1198	the pooled control human serum sample	1162:1198	the pooled control human serum sample	1162:1198	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	1	11	theme	chronic	147:153	arg1	ailments					206:213	prevalent ailments	196:213	prevalent ailments	196:213	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	11	theme	chronic	147:153	arg1	COPD					186:189	COPD	186:189	COPD	186:189	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	11	theme	chronic	147:153	arg1	cancer					131:136	Lung cancer	126:136	Lung cancer (LC)	126:141	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	11	theme	chronic	147:153	arg1	disease					177:183	chronic obstructive pulmonary disease	147:183	chronic obstructive pulmonary disease (COPD)	147:190	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	4	12	theme	COPD	609:612	arg1	LC					631:632	LC	631:632	LC with COPD	631:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	12	theme	COPD	609:612	arg1	patients					645:652	lung cancer, COPD and comorbidity (LC with COPD) patients	596:652	patients	645:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	7	13	with	comorbidity	1261:1271	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	10	14	theme	individual	1742:1751	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	14	theme	individual	1742:1751	arg1	subclasses					1762:1771	the individual N-glycan subclasses	1738:1771	the individual N-glycan subclasses	1738:1771	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	1	15	theme	pulmonary	167:175	arg1	ailments					206:213	prevalent ailments	196:213	prevalent ailments	196:213	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	15	theme	pulmonary	167:175	arg1	COPD					186:189	COPD	186:189	COPD	186:189	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	15	theme	pulmonary	167:175	arg1	cancer					131:136	Lung cancer	126:136	Lung cancer (LC)	126:141	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	15	theme	pulmonary	167:175	arg1	disease					177:183	chronic obstructive pulmonary disease	147:183	chronic obstructive pulmonary disease (COPD)	147:190	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	8	16	located	detected	1377:1384	arg2	structures					1361:1370	no other sugar structures	1346:1370	no other sugar structures	1346:1370	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	8	16	located	detected	1377:1384	arg1	any					1389:1391	any	1389:1391	any	1389:1391	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	0	17	theme	capillary	99:107	arg1	electrophoresis					109:123	capillary electrophoresis	99:123	capillary electrophoresis	99:123	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	9	18	dep	changes	1598:1604	arg1	%					1610:1610	>33%	1607:1610	>33% in relative peak areas	1607:1633	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	0	19	from	comorbidity	81:91	arg1	cancer					58:63	lung cancer	53:63	lung cancer	53:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	5	20	link	N-linked	1034:1041	arg1	carbohydrates					1043:1055	the N-linked carbohydrates	1030:1055	the N-linked carbohydrates	1030:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	0	21	theme	comorbidity	81:91	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity	0:91	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	5	22	theme	N-linked	1034:1041	arg1	carbohydrates					1043:1055	the N-linked carbohydrates	1030:1055	the N-linked carbohydrates	1030:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	5	23	theme	new	883:885	arg1	temperature					887:897	a new temperature	881:897	a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	881:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	10	24	from	alterations	1723:1733	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	24	from	alterations	1723:1733	arg1	subclasses					1762:1771	the individual N-glycan subclasses	1738:1771	the individual N-glycan subclasses	1738:1771	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	4	25	theme	comorbidity	618:628	arg1	LC					631:632	LC	631:632	LC with COPD	631:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	25	theme	comorbidity	618:628	arg1	patients					645:652	lung cancer, COPD and comorbidity (LC with COPD) patients	596:652	patients	645:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	26	with	electrophoresis	736:750	arg1	detection					801:809	high sensitivity laser-induced fluorescence detection	757:809	high sensitivity laser-induced fluorescence detection	757:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	0	27	theme	lung	53:56	arg1	cancer					58:63	lung cancer	53:63	lung cancer	53:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	10	28	theme	total	1782:1786	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	28	theme	total	1782:1786	arg1	degree					1802:1807	degree	1802:1807	degree of sialylation and branching	1802:1836	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	4	29	dep	individuals	697:707	arg1	comparison					675:684	comparison	675:684	comparison	675:684	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	30	dep	samples	564:570	arg1	patients					576:583	90 patients	573:583	90 patients each	573:588	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	5	31	theme	serum	855:859	arg1	samples					861:867	human serum samples	849:867	human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	849:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	4	32	theme	healthy	689:695	arg1	control					710:716	control	710:716	control	710:716	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	32	theme	healthy	689:695	arg1	individuals					697:707	healthy individuals	689:707	healthy individuals (control)	689:717	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	1	33	theme	Lung	126:129	arg1	ailments					206:213	prevalent ailments	196:213	prevalent ailments	196:213	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	33	theme	Lung	126:129	arg1	LC					139:140	LC	139:140	LC	139:140	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	33	theme	Lung	126:129	arg1	disease					177:183	chronic obstructive pulmonary disease	147:183	chronic obstructive pulmonary disease (COPD)	147:190	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	33	theme	Lung	126:129	arg1	cancer					131:136	Lung cancer	126:136	Lung cancer (LC)	126:141	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	0	34	from	N-glycome	40:48	arg1	cancer					58:63	lung cancer	53:63	lung cancer	53:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	4	35	from	profile	534:540	arg1	LC					631:632	LC	631:632	LC with COPD	631:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	35	from	profile	534:540	arg1	patients					645:652	lung cancer, COPD and comorbidity (LC with COPD) patients	596:652	patients	645:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	9	36	theme	structures	1520:1529	arg1	glycobiomarkers					1560:1574	potential glycobiomarkers	1550:1574	potential glycobiomarkers	1550:1574	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	9	36	theme	structures	1520:1529	arg1	panel					1499:1503	a panel	1497:1503	a panel of 13 N-glycan structures	1497:1529	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	0	37	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity	0:91	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	5	38	theme	adjusted	899:906	arg1	protocol					921:928	a new temperature adjusted denaturation protocol	881:928	a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	881:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	7	39	theme	patient	1298:1304	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	4	40	theme	pilot	502:506	arg1	study					508:512	this pilot study	497:512	this pilot study	497:512	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	8	41	theme	sugar	1355:1359	arg1	structures					1361:1370	no other sugar structures	1346:1370	no other sugar structures	1346:1370	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	9	42	theme	potential	1550:1558	arg1	glycobiomarkers					1560:1574	potential glycobiomarkers	1550:1574	potential glycobiomarkers	1550:1574	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	9	42	theme	potential	1550:1558	arg1	panel					1499:1503	a panel	1497:1503	a panel of 13 N-glycan structures	1497:1529	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	7	43	theme	lung	1286:1289	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	0	44	theme	human	28:32	arg1	N-glycome					40:48	the human serum N-glycome	24:48	the human serum N-glycome in lung cancer	24:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	9	45	dep	pathological	1648:1659	arg1	samples					1673:1679	samples	1673:1679	samples	1673:1679	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	7	46	theme	COPD	1276:1279	arg1	COPD					1252:1255	COPD	1252:1255	COPD	1252:1255	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	7	46	theme	COPD	1276:1279	arg1	cancer					1244:1249	pooled lung cancer	1232:1249	pooled lung cancer	1232:1249	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	7	46	theme	COPD	1276:1279	arg1	comorbidity					1261:1271	comorbidity	1261:1271	comorbidity of COPD with lung cancer patient samples	1261:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	4	47	theme	high	757:760	arg1	sensitivity					762:772	high sensitivity	757:772	high sensitivity laser-induced fluorescence detection	757:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	3	48	theme	cancer	444:449	arg1	development					451:461	lung cancer development	439:461	lung cancer development	439:461	Moreover, COPD increases the risk of lung cancer development, leading to their comorbidity.					
31881512	0	49	theme	N-glycome	40:48	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity	0:91	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	4	50	dep	patients	576:583	arg1	each					585:588	each	585:588	each	585:588	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	10	51	theme	profile	1706:1712	arg1	changes					1714:1720	N-glycan profile changes	1697:1720	N-glycan profile changes	1697:1720	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	5	52	theme	endoglycosidase	969:983	arg1	time					995:998	increased endoglycosidase digestion time	959:998	increased endoglycosidase digestion time	959:998	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	1	53	with	ailments	206:213	arg1	challenge					228:236	a great challenge	220:236	a great challenge to distinguish them based on symptoms only	220:279	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	4	54	theme	serum	558:562	arg1	samples					564:570	pooled human serum samples	545:570	pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	545:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	2	55	theme	capable	368:374	arg1	methods					360:366	non-invasive methods	347:366	non-invasive methods capable to readily diagnose them	347:399	Since they require different treatments, it is important to find non-invasive methods capable to readily diagnose them.					
31881512	8	56	theme	patient	1400:1406	arg1	groups					1408:1413	the patient groups	1396:1413	the patient groups	1396:1413	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	10	57	theme	N-glycan	1697:1704	arg1	changes					1714:1720	N-glycan profile changes	1697:1720	N-glycan profile changes	1697:1720	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	5	58	theme	Sample	812:817	arg1	preparation					819:829	Sample preparation	812:829	Sample preparation	812:829	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	6	59	theme	method	1095:1100	arg1	%					1110:1110	<3.5%	1106:1110	<3.5%	1106:1110	The reproducibility of the optimized method was <3.5%.					
31881512	6	59	theme	method	1095:1100	arg1	reproducibility					1062:1076	The reproducibility	1058:1076	The reproducibility of the optimized method	1058:1100	The reproducibility of the optimized method was <3.5%.					
31881512	9	60	theme	relative	1615:1622	arg1	areas					1629:1633	relative peak areas	1615:1633	relative peak areas	1615:1633	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	7	61	theme	pooled	1232:1237	arg1	cancer					1244:1249	pooled lung cancer	1232:1249	pooled lung cancer	1232:1249	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	10	62	theme	glycobiomarker	1862:1875	arg1	values					1877:1882	important glycobiomarker values	1852:1882	important glycobiomarker values	1852:1882	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	4	63	theme	pooled	545:550	arg1	samples					564:570	pooled human serum samples	545:570	pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	545:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	64	theme	sensitivity	762:772	arg1	detection					801:809	high sensitivity laser-induced fluorescence detection	757:809	high sensitivity laser-induced fluorescence detection	757:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	65	theme	lung	596:599	arg1	LC					631:632	LC	631:632	LC with COPD	631:642	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	65	theme	lung	596:599	arg1	patients					645:652	lung cancer, COPD and comorbidity (LC with COPD) patients	596:652	patients	645:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	10	66	theme	N-glycan	1753:1760	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	66	theme	N-glycan	1753:1760	arg1	subclasses					1762:1771	the individual N-glycan subclasses	1738:1771	the individual N-glycan subclasses	1738:1771	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	1	67	theme	obstructive	155:165	arg1	ailments					206:213	prevalent ailments	196:213	prevalent ailments	196:213	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	67	theme	obstructive	155:165	arg1	COPD					186:189	COPD	186:189	COPD	186:189	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	67	theme	obstructive	155:165	arg1	cancer					131:136	Lung cancer	126:136	Lung cancer (LC)	126:141	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	67	theme	obstructive	155:165	arg1	disease					177:183	chronic obstructive pulmonary disease	147:183	chronic obstructive pulmonary disease (COPD)	147:190	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	4	68	theme	fluorescence	788:799	arg1	detection					801:809	high sensitivity laser-induced fluorescence detection	757:809	high sensitivity laser-induced fluorescence detection	757:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	9	69	theme	significant	1586:1596	arg1	changes					1598:1604	significant changes	1586:1604	significant changes (>33% in relative peak areas)	1586:1634	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	0	70	from	COPD	66:69	arg1	cancer					58:63	lung cancer	53:63	lung cancer	53:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	2	71	theme	different	301:309	arg1	treatments					311:320	different treatments	301:320	different treatments	301:320	Since they require different treatments, it is important to find non-invasive methods capable to readily diagnose them.					
31881512	0	72	from	cancer	58:63	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity	0:91	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	4	73	gly	N-glycosylation	518:532	arg1	samples					564:570	pooled human serum samples	545:570	pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	545:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	8	74	theme	important	1319:1327	arg1	finding					1329:1335	One important finding	1315:1335	One important finding	1315:1335	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	5	75	theme	carbohydrates	1043:1055	arg1	removal					1019:1025	complete removal	1010:1025	complete removal of the N-linked carbohydrates	1010:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	0	76	theme	COPD	66:69	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity	0:91	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	5	77	theme	human	849:853	arg1	samples					861:867	human serum samples	849:867	human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	849:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	0	78	from	analysis	12:19	arg1	cancer					58:63	lung cancer	53:63	lung cancer	53:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	9	79	theme	comparative	1475:1485	arg1	exercise					1487:1494	this comparative exercise	1470:1494	this comparative exercise	1470:1494	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	7	80	theme	human	1181:1185	arg1	sample					1193:1198	the pooled control human serum sample	1162:1198	the pooled control human serum sample	1162:1198	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	8	81	theme	quantitative	1421:1432	arg1	differences					1434:1444	only quantitative differences	1416:1444	only quantitative differences	1416:1444	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	9	82	from	%	1610:1610	arg1	areas					1629:1633	relative peak areas	1615:1633	relative peak areas	1615:1633	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	2	83	theme	non-invasive	347:358	arg1	methods					360:366	non-invasive methods	347:366	non-invasive methods capable to readily diagnose them	347:399	Since they require different treatments, it is important to find non-invasive methods capable to readily diagnose them.					
31881512	7	84	theme	pooled	1166:1171	arg1	sample					1193:1198	the pooled control human serum sample	1162:1198	the pooled control human serum sample	1162:1198	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	5	85	theme	temperature	887:897	arg1	protocol					921:928	a new temperature adjusted denaturation protocol	881:928	a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	881:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	7	86	theme	cancer	1291:1296	arg1	samples					1306:1312	lung cancer patient samples	1286:1312	lung cancer patient samples	1286:1312	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	4	87	theme	capillary	722:730	arg1	electrophoresis					736:750	capillary gel electrophoresis	722:750	capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection	722:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	5	88	theme	denaturation	908:919	arg1	protocol					921:928	a new temperature adjusted denaturation protocol	881:928	a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates	881:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	4	89	theme	N-glycosylation	518:532	arg1	profile					534:540	the N-glycosylation profile	514:540	the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	514:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	9	90	theme	N-glycan	1511:1518	arg1	structures					1520:1529	13 N-glycan structures	1508:1529	13 N-glycan structures	1508:1529	Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (>33% in relative peak areas) between the pathological and control samples.					
31881512	10	91	theme	branching	1828:1836	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	91	theme	branching	1828:1836	arg1	degree					1802:1807	degree	1802:1807	degree of sialylation and branching	1802:1836	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	8	92	theme	other	1349:1353	arg1	structures					1361:1370	no other sugar structures	1346:1370	no other sugar structures	1346:1370	One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed.					
31881512	0	93	theme	serum	34:38	arg1	N-glycome					40:48	the human serum N-glycome	24:48	the human serum N-glycome in lung cancer	24:63	Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis.					
31881512	10	94	theme	sialylation	1812:1822	arg1	fucosylation					1788:1799	total fucosylation	1782:1799	total fucosylation	1782:1799	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	10	94	theme	sialylation	1812:1822	arg1	degree					1802:1807	degree	1802:1807	degree of sialylation and branching	1802:1836	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	4	95	theme	gel	732:734	arg1	electrophoresis					736:750	capillary gel electrophoresis	722:750	capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection	722:809	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	7	96	theme	serum	1187:1191	arg1	sample					1193:1198	the pooled control human serum sample	1162:1198	the pooled control human serum sample	1162:1198	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	7	97	theme	N-glycan	1123:1130	arg1	structures					1132:1141	Sixty-one N-glycan structures	1113:1141	Sixty-one N-glycan structures	1113:1141	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
31881512	5	98	theme	complete	1010:1017	arg1	removal					1019:1025	complete removal	1010:1025	complete removal of the N-linked carbohydrates	1010:1055	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	10	99	theme	important	1852:1860	arg1	values					1877:1882	important glycobiomarker values	1852:1882	important glycobiomarker values	1852:1882	In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.					
31881512	3	100	theme	development	451:461	arg1	risk					431:434	the risk	427:434	the risk of lung cancer development	427:461	Moreover, COPD increases the risk of lung cancer development, leading to their comorbidity.					
31881512	4	101	from	patients	645:652	arg1	samples					564:570	pooled human serum samples	545:570	pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	545:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	4	101	from	patients	645:652	arg1	profile					534:540	the N-glycosylation profile	514:540	the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	514:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	5	102	theme	increased	959:967	arg1	time					995:998	increased endoglycosidase digestion time	959:998	increased endoglycosidase digestion time	959:998	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	4	103	theme	samples	564:570	arg1	profile					534:540	the N-glycosylation profile	514:540	the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients	514:652	In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection.					
31881512	3	104	theme	lung	439:442	arg1	development					451:461	lung cancer development	439:461	lung cancer development	439:461	Moreover, COPD increases the risk of lung cancer development, leading to their comorbidity.					
31881512	1	105	theme	prevalent	196:204	arg1	ailments					206:213	prevalent ailments	196:213	prevalent ailments	196:213	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	105	theme	prevalent	196:204	arg1	disease					177:183	chronic obstructive pulmonary disease	147:183	chronic obstructive pulmonary disease (COPD)	147:190	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	1	105	theme	prevalent	196:204	arg1	cancer					131:136	Lung cancer	126:136	Lung cancer (LC)	126:141	Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only.					
31881512	5	106	theme	digestion	985:993	arg1	time					995:998	increased endoglycosidase digestion time	959:998	increased endoglycosidase digestion time	959:998	Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates.					
31881512	7	107	theme	lung	1239:1242	arg1	cancer					1244:1249	pooled lung cancer	1232:1249	pooled lung cancer	1232:1249	Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples.					
30988258	6	0	theme	modification	969:980	arg1	function					942:949	the biological function	927:949	the biological function of this important modification	927:980	With better modeling of N-glycosylation, the biological function of this important modification can be better and more easily understood.					
30988258	5	1	theme	carbohydrate	756:767	arg1	residues					769:776	16 452 carbohydrate residues	749:776	16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models	749:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	2	from	residues	769:776	arg1	trees					802:806	11 651 glycosylation trees	781:806	11 651 glycosylation trees in 4498 structure models	781:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	3	from	server	878:883	arg1	available					846:854	available	846:854	available	846:854	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	3	from	server	878:883	arg1	method					654:659	The method	650:659	The method	650:659	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	4	4	theme	glycosylation	527:539	arg1	trees					541:545	existing glycosylation trees	518:545	existing glycosylation trees	518:545	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	2	5	theme	residues	301:308	arg1	trees					249:253	N-Glycosylation trees	233:253	N-Glycosylation trees	233:253	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	2	5	theme	residues	301:308	arg1	structures					267:276	complex structures	259:276	complex structures of linked carbohydrate residues attached to asparagine residues	259:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	4	6	theme	glycosylation	594:606	arg1	trees					608:612	new glycosylation trees	590:612	new glycosylation trees if they are missing from the model	590:647	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	3	7	theme	protein	388:394	arg1	structures					396:405	protein structures	388:405	protein structures	388:405	While carbohydrates are typically modeled in protein structures, they are often incomplete or have the wrong chemistry.					
30988258	3	8	contain	have	437:440	arg1	they					408:411	they	408:411	they	408:411	While carbohydrates are typically modeled in protein structures, they are often incomplete or have the wrong chemistry.					
30988258	3	8	contain	have	437:440	arg2	chemistry					452:460	the wrong chemistry	442:460	the wrong chemistry	442:460	While carbohydrates are typically modeled in protein structures, they are often incomplete or have the wrong chemistry.					
30988258	2	9	theme	carbohydrate	288:299	arg1	residues					301:308	linked carbohydrate residues	281:308	linked carbohydrate residues attached to asparagine residues	281:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	4	10	theme	new	590:592	arg1	trees					608:612	new glycosylation trees	590:612	new glycosylation trees if they are missing from the model	590:647	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	4	11	theme	existing	518:525	arg1	trees					541:545	existing glycosylation trees	518:545	existing glycosylation trees	518:545	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	2	12	theme	linked	281:286	arg1	residues					301:308	linked carbohydrate residues	281:308	linked carbohydrate residues attached to asparagine residues	281:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	5	13	theme	glycosylation	788:800	arg1	trees					802:806	11 651 glycosylation trees	781:806	11 651 glycosylation trees in 4498 structure models	781:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	14	from	trees	802:806	arg1	models					826:831	4498 structure models	811:831	4498 structure models	811:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	6	15	theme	important	959:967	arg1	modification					969:980	this important modification	954:980	this important modification	954:980	With better modeling of N-glycosylation, the biological function of this important modification can be better and more easily understood.					
30988258	1	16	theme	protein	173:179	arg1	folding					181:187	protein folding	173:187	protein folding	173:187	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	6	17	theme	better	891:896	arg1	modeling					898:905	better modeling	891:905	better modeling of N-glycosylation	891:924	With better modeling of N-glycosylation, the biological function of this important modification can be better and more easily understood.					
30988258	2	18	link	linked	281:286	arg1	residues					301:308	linked carbohydrate residues	281:308	linked carbohydrate residues attached to asparagine residues	281:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	2	19	theme	asparagine	322:331	arg1	residues					333:340	asparagine residues	322:340	asparagine residues	322:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	4	20	from	model	643:647	arg1	missing					626:632	missing	626:632	missing	626:632	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	3	21	theme	wrong	446:450	arg1	chemistry					452:460	the wrong chemistry	442:460	the wrong chemistry	442:460	While carbohydrates are typically modeled in protein structures, they are often incomplete or have the wrong chemistry.					
30988258	5	22	theme	4498	811:814	arg1	models					826:831	4498 structure models	811:831	4498 structure models	811:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	23	theme	PDB-REDO	690:697	arg1	pipeline					699:706	the PDB-REDO pipeline	686:706	the PDB-REDO pipeline	686:706	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	5	24	theme	structure	816:824	arg1	models					826:831	4498 structure models	811:831	4498 structure models	811:831	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	2	25	attach	attached	310:317	arg2	residues					301:308	linked carbohydrate residues	281:308	linked carbohydrate residues attached to asparagine residues	281:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	2	25	attach	attached	310:317	arg1	residues					333:340	asparagine residues	322:340	asparagine residues	322:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	1	26	theme	common	98:103	arg1	modifications					124:136	the most common post-translational modifications	89:136	the most common post-translational modifications	89:136	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	0	27	theme	protein	37:43	arg1	models					55:60	protein structure models	37:60	protein structure models	37:60	Building and rebuilding N-glycans in protein structure models.					
30988258	2	28	theme	N-Glycosylation	233:247	arg1	trees					249:253	N-Glycosylation trees	233:253	N-Glycosylation trees	233:253	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	2	28	theme	N-Glycosylation	233:247	arg1	structures					267:276	complex structures	259:276	complex structures of linked carbohydrate residues attached to asparagine residues	259:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	6	29	theme	biological	931:940	arg1	function					942:949	the biological function	927:949	the biological function of this important modification	927:980	With better modeling of N-glycosylation, the biological function of this important modification can be better and more easily understood.					
30988258	1	30	with	interaction	193:203	arg1	receptors					222:230	receptors	222:230	receptors	222:230	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	1	30	with	interaction	193:203	arg1	ligands					210:216	ligands	210:216	ligands	210:216	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	4	31	theme	new	469:471	arg1	tools					473:477	new tools	469:477	new tools	469:477	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	1	32	theme	post-translational	105:122	arg1	modifications					124:136	the most common post-translational modifications	89:136	the most common post-translational modifications	89:136	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	0	33	theme	structure	45:53	arg1	models					55:60	protein structure models	37:60	protein structure models	37:60	Building and rebuilding N-glycans in protein structure models.					
30988258	1	34	with	folding	181:187	arg1	receptors					222:230	receptors	222:230	receptors	222:230	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	1	34	with	folding	181:187	arg1	ligands					210:216	ligands	210:216	ligands	210:216	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	1	35	theme	modifications	124:136	arg1	one					82:84	one	82:84	one	82:84	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	1	35	theme	modifications	124:136	arg1	modifications					124:136	the most common post-translational modifications	89:136	the most common post-translational modifications	89:136	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	6	36	theme	N-glycosylation	910:924	arg1	modeling					898:905	better modeling	891:905	better modeling of N-glycosylation	891:924	With better modeling of N-glycosylation, the biological function of this important modification can be better and more easily understood.					
30988258	2	37	theme	complex	259:265	arg1	trees					249:253	N-Glycosylation trees	233:253	N-Glycosylation trees	233:253	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	2	37	theme	complex	259:265	arg1	structures					267:276	complex structures	259:276	complex structures of linked carbohydrate residues attached to asparagine residues	259:340	N-Glycosylation trees are complex structures of linked carbohydrate residues attached to asparagine residues.					
30988258	1	38	gly	N-Glycosylation	63:77	arg1	interaction					193:203	interaction	193:203	interaction with ligands and receptors	193:230	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	1	38	gly	N-Glycosylation	63:77	arg1	folding					181:187	protein folding	173:187	protein folding	173:187	N-Glycosylation is one of the most common post-translational modifications and is implicated in, for example, protein folding and interaction with ligands and receptors.					
30988258	4	39	dep	trees	608:612	arg1	missing					626:632	missing	626:632	missing	626:632	Here, new tools are presented to automatically rebuild existing glycosylation trees, to extend them where possible, and to add new glycosylation trees if they are missing from the model.					
30988258	5	40	theme	PDB-REDO	865:872	arg1	server					878:883	the PDB-REDO web server	861:883	the PDB-REDO web server	861:883	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
30988258	0	41	from	N-glycans	24:32	arg1	models					55:60	protein structure models	37:60	protein structure models	37:60	Building and rebuilding N-glycans in protein structure models.					
30988258	5	42	theme	web	874:876	arg1	server					878:883	the PDB-REDO web server	861:883	the PDB-REDO web server	861:883	The method has been incorporated in the PDB-REDO pipeline and has been applied to build or rebuild 16 452 carbohydrate residues in 11 651 glycosylation trees in 4498 structure models, and is also available from the PDB-REDO web server.					
29842789	6	0	theme	inhibitory	1037:1046	arg1	activities					1048:1057	significant inhibitory activities	1025:1057	significant inhibitory activities	1025:1057	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	6	1	theme	significant	1025:1035	arg1	activities					1048:1057	significant inhibitory activities	1025:1057	significant inhibitory activities	1025:1057	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	1	2	theme	Circinella	165:174	arg1	muscae					176:181	Circinella muscae	165:181	Circinella muscae AS 3.2695	165:191	Biotransformation of oleanolic acid (OA) by Circinella muscae AS 3.2695 was investigated.					
29842789	0	3	theme	anti-inflammatory	91:107	arg1	activities					109:118	their anti-inflammatory activities	85:118	their anti-inflammatory activities	85:118	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	3	4	theme	3β,7β,21β-trihydroxyolean-12-en-28-oic	359:396	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	4	theme	3β,7β,21β-trihydroxyolean-12-en-28-oic	359:396	arg1	acid					398:401	3β,7β,21β-trihydroxyolean-12-en-28-oic acid	359:401	3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2)	359:405	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	4	theme	3β,7β,21β-trihydroxyolean-12-en-28-oic	359:396	arg1	2					404:404	2	404:404	2	404:404	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	4	5	theme	new	877:879	arg1	compounds					881:889	new compounds	877:889	new compounds	877:889	Among them, compounds 4 and 9 were new compounds.					
29842789	0	6	theme	acid	55:58	arg1	glycosidation					28:40	glycosidation	28:40	glycosidation	28:40	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	0	6	theme	acid	55:58	arg1	hydroxylation					10:22	Microbial hydroxylation	0:22	Microbial hydroxylation	0:22	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	3	7	theme	spectroscopic	818:830	arg1	analysis					832:839	spectroscopic analysis	818:839	spectroscopic analysis	818:839	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	8	theme	28-oic	441:446	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	8	theme	28-oic	441:446	arg1	3					454:454	3	454:454	3	454:454	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	8	theme	28-oic	441:446	arg1	acid					448:451	3β,7α,21β-trihydroxyolean-12-en- 28-oic acid	408:451	3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3)	408:455	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	5	9	theme	isolated	969:976	arg1	metabolites					978:988	the isolated metabolites	965:988	the isolated metabolites	965:988	In addition, anti-inflammatory activities were assayed and evaluated for the isolated metabolites.					
29842789	3	10	theme	3β,7α,21β-trihydroxyolean-12-en-	408:439	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	10	theme	3β,7α,21β-trihydroxyolean-12-en-	408:439	arg1	3					454:454	3	454:454	3	454:454	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	10	theme	3β,7α,21β-trihydroxyolean-12-en-	408:439	arg1	acid					448:451	3β,7α,21β-trihydroxyolean-12-en- 28-oic acid	408:451	3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3)	408:455	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	0	11	theme	Microbial	0:8	arg1	hydroxylation					10:22	Microbial hydroxylation	0:22	Microbial hydroxylation	0:22	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	3	12	theme	3β,7β,15α-trihydroxyolean-12-en-28-oic	458:495	arg1	acid					497:500	3β,7β,15α-trihydroxyolean-12-en-28-oic acid	458:500	3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4)	458:504	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	12	theme	3β,7β,15α-trihydroxyolean-12-en-28-oic	458:495	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	12	theme	3β,7β,15α-trihydroxyolean-12-en-28-oic	458:495	arg1	4					503:503	4	503:503	4	503:503	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	5	13	theme	anti-inflammatory	905:921	arg1	activities					923:932	anti-inflammatory activities	905:932	anti-inflammatory activities	905:932	In addition, anti-inflammatory activities were assayed and evaluated for the isolated metabolites.					
29842789	3	14	theme	oleanolic	606:614	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	14	theme	oleanolic	606:614	arg1	7					652:652	7	652:652	7	652:652	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	14	theme	oleanolic	606:614	arg1	ester					645:649	oleanolic acid-28-O-β-D-glucopyranosyl ester	606:649	oleanolic acid-28-O-β-D-glucopyranosyl ester (7)	606:653	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	6	15	theme	RAW	1107:1109	arg1	cells					1117:1121	RAW 264.7 cells	1107:1121	RAW 264.7 cells	1107:1121	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	3	16	theme	acid-28-O-β-D-glucopyranosyl	616:643	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	16	theme	acid-28-O-β-D-glucopyranosyl	616:643	arg1	7					652:652	7	652:652	7	652:652	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	16	theme	acid-28-O-β-D-glucopyranosyl	616:643	arg1	ester					645:649	oleanolic acid-28-O-β-D-glucopyranosyl ester	606:649	oleanolic acid-28-O-β-D-glucopyranosyl ester (7)	606:653	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	17	theme	7β,15α-dihydroxy-	507:523	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	17	theme	7β,15α-dihydroxy-	507:523	arg1	5					556:556	5	556:556	5	556:556	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	17	theme	7β,15α-dihydroxy-	507:523	arg1	acid					550:553	7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid	507:553	7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5)	507:557	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	0	18	theme	oleanolic	45:53	arg1	acid					55:58	oleanolic acid	45:58	oleanolic acid	45:58	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	3	19	theme	3β,21β-dihydroxyolean-12-en-28-	656:686	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	19	theme	3β,21β-dihydroxyolean-12-en-28-	656:686	arg1	8					728:728	8	728:728	8	728:728	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	19	theme	3β,21β-dihydroxyolean-12-en-28-	656:686	arg1	ester					721:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester	656:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8)	656:729	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	6	20	theme	NO	1090:1091	arg1	production					1093:1102	lipopolysaccharides-induced NO production	1062:1102	lipopolysaccharides-induced NO production in RAW 264.7 cells	1062:1121	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	3	21	theme	3β,7β-dihydroxyolean-12-en-28-oic	315:347	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	21	theme	3β,7β-dihydroxyolean-12-en-28-oic	315:347	arg1	1					355:355	1	355:355	1	355:355	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	21	theme	3β,7β-dihydroxyolean-12-en-28-oic	315:347	arg1	acid					349:352	3β,7β-dihydroxyolean-12-en-28-oic acid	315:352	3β,7β-dihydroxyolean-12-en-28-oic acid (1)	315:356	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	2	22	theme	glycosylated	233:244	arg1	metabolites					246:256	glycosylated metabolites	233:256	glycosylated metabolites	233:256	Nine hydroxylated and glycosylated metabolites (1-9) were obtained.					
29842789	2	23	gly	glycosylated	233:244	arg1	metabolites					246:256	glycosylated metabolites	233:256	glycosylated metabolites	233:256	Nine hydroxylated and glycosylated metabolites (1-9) were obtained.					
29842789	6	24	theme	lipopolysaccharides-induced	1062:1088	arg1	production					1093:1102	lipopolysaccharides-induced NO production	1062:1102	lipopolysaccharides-induced NO production in RAW 264.7 cells	1062:1121	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	3	25	theme	acid-28-O-β-D-glucopyranosyl	776:803	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	25	theme	acid-28-O-β-D-glucopyranosyl	776:803	arg1	9					812:812	9	812:812	9	812:812	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	25	theme	acid-28-O-β-D-glucopyranosyl	776:803	arg1	ester					805:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester	736:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9)	736:813	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	1	26	theme	oleanolic	142:150	arg1	acid					152:155	oleanolic acid	142:155	oleanolic acid (OA)	142:160	Biotransformation of oleanolic acid (OA) by Circinella muscae AS 3.2695 was investigated.					
29842789	1	26	theme	oleanolic	142:150	arg1	OA					158:159	OA	158:159	OA	158:159	Biotransformation of oleanolic acid (OA) by Circinella muscae AS 3.2695 was investigated.					
29842789	6	27	from	production	1093:1102	arg1	cells					1117:1121	RAW 264.7 cells	1107:1121	RAW 264.7 cells	1107:1121	Most of the metabolites exhibited significant inhibitory activities on lipopolysaccharides-induced NO production in RAW 264.7 cells.					
29842789	1	28	theme	acid	152:155	arg1	Biotransformation					121:137	Biotransformation	121:137	Biotransformation of oleanolic acid (OA) by Circinella muscae AS 3.2695	121:191	Biotransformation of oleanolic acid (OA) by Circinella muscae AS 3.2695 was investigated.					
29842789	3	29	theme	3β,7β,15α-trihydroxyolean-12-en-	736:767	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	29	theme	3β,7β,15α-trihydroxyolean-12-en-	736:767	arg1	9					812:812	9	812:812	9	812:812	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	29	theme	3β,7β,15α-trihydroxyolean-12-en-	736:767	arg1	ester					805:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester	736:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9)	736:813	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	30	theme	3-oxo-olean-12-en-28-oic	525:548	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	30	theme	3-oxo-olean-12-en-28-oic	525:548	arg1	5					556:556	5	556:556	5	556:556	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	30	theme	3-oxo-olean-12-en-28-oic	525:548	arg1	acid					550:553	7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid	507:553	7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5)	507:557	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	31	theme	28-oic	769:774	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	31	theme	28-oic	769:774	arg1	9					812:812	9	812:812	9	812:812	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	31	theme	28-oic	769:774	arg1	ester					805:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester	736:809	3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9)	736:813	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	32	theme	oic	688:690	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	32	theme	oic	688:690	arg1	8					728:728	8	728:728	8	728:728	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	32	theme	oic	688:690	arg1	ester					721:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester	656:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8)	656:729	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	33	theme	7β-hydroxy-3-oxo-olean-12-en-28-oic	560:594	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	33	theme	7β-hydroxy-3-oxo-olean-12-en-28-oic	560:594	arg1	6					602:602	6	602:602	6	602:602	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	33	theme	7β-hydroxy-3-oxo-olean-12-en-28-oic	560:594	arg1	acid					596:599	7β-hydroxy-3-oxo-olean-12-en-28-oic acid	560:599	7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6)	560:603	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	0	34	theme	Circinella	63:72	arg1	muscae					74:79	Circinella muscae	63:79	Circinella muscae	63:79	Microbial hydroxylation and glycosidation of oleanolic acid by Circinella muscae and their anti-inflammatory activities.					
29842789	3	35	theme	acid-28-O-β-D-glucopyranosyl	692:719	arg1	structures					285:294	Their structures	279:294	Their structures	279:294	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	35	theme	acid-28-O-β-D-glucopyranosyl	692:719	arg1	8					728:728	8	728:728	8	728:728	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
29842789	3	35	theme	acid-28-O-β-D-glucopyranosyl	692:719	arg1	ester					721:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester	656:725	3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8)	656:729	Their structures were elucidated as 3β,7β-dihydroxyolean-12-en-28-oic acid (1), 3β,7β,21β-trihydroxyolean-12-en-28-oic acid (2), 3β,7α,21β-trihydroxyolean-12-en- 28-oic acid (3), 3β,7β,15α-trihydroxyolean-12-en-28-oic acid (4), 7β,15α-dihydroxy- 3-oxo-olean-12-en-28-oic acid (5), 7β-hydroxy-3-oxo-olean-12-en-28-oic acid (6), oleanolic acid-28-O-β-D-glucopyranosyl ester (7), 3β,21β-dihydroxyolean-12-en-28- oic acid-28-O-β-D-glucopyranosyl ester (8), and 3β,7β,15α-trihydroxyolean-12-en- 28-oic acid-28-O-β-D-glucopyranosyl ester (9) by spectroscopic analysis.					
31053724	7	0	theme	glycan	1125:1130	arg1	structures					1132:1141	glycan structures	1125:1141	glycan structures much smaller than the theoretical one	1125:1179	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	7	1	theme	enzyme	1038:1043	arg1	combinations					1045:1056	a few thousand enzyme combinations	1023:1056	a few thousand enzyme combinations for glycan breakdown	1023:1077	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	2	2	theme	glycan	331:336	arg1	structures					338:347	glycan structures	331:347	glycan structures	331:347	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	7	3	theme	structures	1132:1141	arg1	diversity					1112:1120	diversity	1112:1120	diversity of glycan structures much smaller than the theoretical one	1112:1179	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	7	4	dep	global	1093:1098	arg1	estimate					1100:1107	estimate	1100:1107	estimate	1100:1107	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	5	5	from	composition	775:785	arg1	PULs					790:793	PULs	790:793	PULs	790:793	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	7	6	theme	diversity	1112:1120	arg1	global					1093:1098	global	1093:1098	global	1093:1098	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	2	7	theme	isomers	270:276	arg1	number					251:256	an astronomical number	235:256	an astronomical number of possible isomers (> 1012 hexasaccharides)	235:301	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	5	8	from	information	807:817	arg1	structure					826:834	the structure	822:834	the structure of the targeted glycans	822:858	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	2	9	from	diversity	318:326	arg1	nature					352:357	nature	352:357	nature	352:357	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	2	10	theme	possible	261:268	arg1	isomers					270:276	possible isomers	261:276	possible isomers (> 1012 hexasaccharides)	261:301	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	2	10	theme	possible	261:268	arg1	hexasaccharides					286:300	> 1012 hexasaccharides	279:300	> 1012 hexasaccharides	279:300	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	5	11	theme	bespoke	735:741	arg1	enzymes					743:749	bespoke enzymes	735:749	bespoke enzymes	735:749	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	4	12	theme	polysaccharide	617:630	arg1	loci					644:647	polysaccharide utilization loci	617:647	polysaccharide utilization loci (PULs)	617:654	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	4	12	theme	polysaccharide	617:630	arg1	PULs					650:653	PULs	650:653	PULs	650:653	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	5	13	theme	targeted	843:850	arg1	glycans					852:858	the targeted glycans	839:858	the targeted glycans	839:858	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	5	14	theme	enzyme	768:773	arg1	composition					775:785	the enzyme composition	764:785	the enzyme composition in PULs	764:793	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	5	15	theme	glycans	852:858	arg1	structure					826:834	the structure	822:834	the structure of the targeted glycans	822:858	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	3	16	theme	Bacteroidetes	389:401	arg1	Bacteria					373:380	Bacteria	373:380	Bacteria of the Bacteroidetes phylum	373:408	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	4	17	theme	carbohydrate-degrading	539:560	arg1	CAZymes					571:577	CAZymes	571:577	CAZymes	571:577	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	4	17	theme	carbohydrate-degrading	539:560	arg1	enzymes					562:568	carbohydrate-degrading enzymes	539:568	carbohydrate-degrading enzymes (CAZymes)	539:578	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	7	18	theme	theoretical	1165:1175	arg1	one					1177:1179	the theoretical one	1161:1179	the theoretical one	1161:1179	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	5	19	theme	complex	689:695	arg1	glycan					697:702	a given complex glycan	681:702	a given complex glycan	681:702	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	1	20	theme	glycan	91:96	arg1	chains					98:103	glycan chains	91:103	glycan chains	91:103	Unlike proteins, glycan chains are not directly encoded by DNA, but by the specificity of the enzymes that assemble them.					
31053724	4	21	theme	utilization	632:642	arg1	loci					644:647	polysaccharide utilization loci	617:647	polysaccharide utilization loci (PULs)	617:654	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	4	21	theme	utilization	632:642	arg1	PULs					650:653	PULs	650:653	PULs	650:653	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	5	22	theme	glycan	697:702	arg1	depolymerization					661:676	The depolymerization	657:676	The depolymerization of a given complex glycan by Bacteroidetes PULs	657:724	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	7	23	dep	collectively	980:991	arg1	Bacteroidetes					993:1005	Bacteroidetes	993:1005	Bacteroidetes	993:1005	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	1	24	theme	enzymes	168:174	arg1	specificity					149:159	the specificity	145:159	the specificity of the enzymes that assemble them	145:193	Unlike proteins, glycan chains are not directly encoded by DNA, but by the specificity of the enzymes that assemble them.					
31053724	0	25	theme	combinations	38:49	arg1	thousands					18:26	thousands	18:26	thousands of enzyme combinations	18:49	Bacteroidetes use thousands of enzyme combinations to break down glycans.					
31053724	6	26	theme	CAZyme	947:952	arg1	composition					954:964	their CAZyme composition	941:964	their CAZyme composition	941:964	Here we group the 13,537 PULs encoded by 964 Bacteroidetes genomes according to their CAZyme composition.					
31053724	7	27	theme	glycan	1062:1067	arg1	breakdown					1069:1077	glycan breakdown	1062:1077	glycan breakdown	1062:1077	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	3	28	dep	Bacteroidetes	389:401	arg1	phylum					403:408	phylum	403:408	phylum	403:408	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	3	29	theme	primary	425:431	arg1	degraders					433:441	primary degraders	425:441	primary degraders of polysaccharides	425:460	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	0	30	theme	enzyme	31:36	arg1	combinations					38:49	enzyme combinations	31:49	enzyme combinations	31:49	Bacteroidetes use thousands of enzyme combinations to break down glycans.					
31053724	5	31	theme	Bacteroidetes	707:719	arg1	PULs					721:724	Bacteroidetes PULs	707:724	Bacteroidetes PULs	707:724	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	2	32	theme	Theoretical	196:206	arg1	calculations					208:219	Theoretical calculations	196:219	Theoretical calculations	196:219	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	7	33	theme	few	1025:1027	arg1	combinations					1045:1056	a few thousand enzyme combinations	1023:1056	a few thousand enzyme combinations for glycan breakdown	1023:1077	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	3	34	theme	polysaccharides	446:460	arg1	degraders					433:441	primary degraders	425:441	primary degraders of polysaccharides	425:460	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	7	35	theme	smaller	1148:1154	arg1	structures					1132:1141	glycan structures	1125:1141	glycan structures much smaller than the theoretical one	1125:1179	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	2	36	theme	actual	311:316	arg1	diversity					318:326	the actual diversity	307:326	the actual diversity of glycan structures in nature	307:357	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	4	37	theme	Bacteroidetes	516:528	arg1	genomes					530:536	Bacteroidetes genomes	516:536	Bacteroidetes genomes	516:536	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	7	38	theme	thousand	1029:1036	arg1	combinations					1045:1056	a few thousand enzyme combinations	1023:1056	a few thousand enzyme combinations for glycan breakdown	1023:1077	We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.					
31053724	2	39	theme	astronomical	238:249	arg1	number					251:256	an astronomical number	235:256	an astronomical number of possible isomers (> 1012 hexasaccharides)	235:301	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
31053724	4	40	theme	gene	596:599	arg1	clusters					601:608	gene clusters	596:608	gene clusters termed polysaccharide utilization loci (PULs)	596:654	In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs).					
31053724	5	41	theme	given	683:687	arg1	glycan					697:702	a given complex glycan	681:702	a given complex glycan	681:702	The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans.					
31053724	3	42	located	found	475:479	arg1	ecosystems					488:497	all ecosystems	484:497	all ecosystems investigated	484:510	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	3	42	located	found	475:479	arg2	they					466:469	they	466:469	they	466:469	Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated.					
31053724	0	43	theme	down	60:63	arg1	glycans					65:71	down glycans	60:71	down glycans	60:71	Bacteroidetes use thousands of enzyme combinations to break down glycans.					
31053724	6	44	theme	Bacteroidetes	906:918	arg1	genomes					920:926	964 Bacteroidetes genomes	902:926	964 Bacteroidetes genomes according to their CAZyme composition	902:964	Here we group the 13,537 PULs encoded by 964 Bacteroidetes genomes according to their CAZyme composition.					
31053724	2	45	theme	structures	338:347	arg1	diversity					318:326	the actual diversity	307:326	the actual diversity of glycan structures in nature	307:357	Theoretical calculations have proposed an astronomical number of possible isomers (> 1012 hexasaccharides) but the actual diversity of glycan structures in nature is not known.					
29409697	1	0	theme	N-glycans	394:402	arg1	core					386:389	the trimanosyl core	371:389	the trimanosyl core of N-glycans	371:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	4	1	theme	120 μg	994:999	arg1	yield					971:975	a yield	969:975	a yield of approximately 120 μg from 10 mL hemolymph	969:1020	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	5	2	theme	enzymatic	1149:1157	arg1	cleavage					1159:1166	the enzymatic cleavage	1145:1166	the enzymatic cleavage of glycans	1145:1177	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	3	3	theme	N-terminal	788:797	arg1	tail					809:812	the N-terminal cytosolic tail	784:812	the N-terminal cytosolic tail	784:812	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	4	4	from	hemolymph	1012:1020	arg1	yield					971:975	a yield	969:975	a yield of approximately 120 μg from 10 mL hemolymph	969:1020	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	6	5	theme	glycans	1444:1450	arg1	removal					1429:1435	the removal	1425:1435	the removal of the glycans	1425:1450	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	1	6	theme	GnTII	233:237	arg1	EC					240:241	GnTII, EC 2.4.1.143	233:251	EC	240:241	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	6	theme	GnTII	233:237	arg1	β-1,2-N-Acetylglucosaminyltransferase					191:227	β-1,2-N-Acetylglucosaminyltransferase II	191:230	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143)	191:252	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	0	7	theme	Bombyx	132:137	arg1	nucleopolyhedrovirus					144:163	a silkworm-based Bombyx mori nucleopolyhedrovirus	115:163	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	4	8	theme	silkworm	881:888	arg1	hemolymph					897:905	silkworm larval hemolymph	881:905	silkworm larval hemolymph	881:905	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	5	9	gly	glycosylated	1247:1258	arg1	hGnTII					1186:1191	hGnTII	1186:1191	hGnTII expressed in insect cells	1186:1217	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	0	10	theme	silkworm-based	117:130	arg1	nucleopolyhedrovirus					144:163	a silkworm-based Bombyx mori nucleopolyhedrovirus	115:163	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	1	11	theme	essential	408:416	arg1	enzyme					297:302	a Golgi-localized type II transmembrane enzyme	257:302	a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans	257:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	11	theme	essential	408:416	arg1	step					418:421	an essential step	405:421	an essential step in the conversion of oligomannose-type to complex-type N-glycans	405:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	4	12	theme	larval	890:895	arg1	hemolymph					897:905	silkworm larval hemolymph	881:905	silkworm larval hemolymph	881:905	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	1	13	dep	N-glycans	478:486	arg1	to					462:463	to	462:463	to	462:463	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	0	14	theme	nucleopolyhedrovirus	144:163	arg1	system					183:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	3	15	theme	transmembrane	818:830	arg1	region					832:837	transmembrane region	818:837	transmembrane region	818:837	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	4	16	theme	affinity	936:943	arg1	purification					945:956	tandem affinity purification	929:956	tandem affinity purification tags	929:961	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	4	17	theme	10 mL	1006:1010	arg1	hemolymph					1012:1020	10 mL hemolymph	1006:1020	10 mL hemolymph	1006:1020	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	0	18	theme	mori	139:142	arg1	nucleopolyhedrovirus					144:163	a silkworm-based Bombyx mori nucleopolyhedrovirus	115:163	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	3	19	theme	cytosolic	799:807	arg1	tail					809:812	the N-terminal cytosolic tail	784:812	the N-terminal cytosolic tail	784:812	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	4	20	theme	tandem	929:934	arg1	purification					945:956	tandem affinity purification	929:956	tandem affinity purification tags	929:961	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	2	21	theme	heterologous	569:580	arg1	expression					582:591	heterologous expression	569:591	heterologous expression	569:591	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	6	22	theme	recombinant	1361:1371	arg1	hGnTII					1373:1378	the recombinant hGnTII	1357:1378	the recombinant hGnTII	1357:1378	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	0	23	theme	Heterologous	0:11	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression	0:22	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	1	24	theme	N-acetylglucosamine	335:353	arg1	transfer					323:330	the transfer	319:330	the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans	319:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	4	25	theme	purification	945:956	arg1	tags					958:961	tandem affinity purification tags	929:961	tandem affinity purification tags	929:961	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	2	26	theme	physiological	501:513	arg1	importance					515:524	its physiological importance	497:524	its physiological importance	497:524	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	0	27	theme	expression	172:181	arg1	system					183:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	4	28	theme	recombinant	844:854	arg1	hGnTII					856:861	The recombinant hGnTII	840:861	The recombinant hGnTII	840:861	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	1	29	from	step	418:421	arg1	conversion					430:439	the conversion	426:439	the conversion of oligomannose-type to complex-type N-glycans	426:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	0	30	theme	bacmid	165:170	arg1	system					183:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	115:188	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	5	31	theme	glycans	1171:1177	arg1	cleavage					1159:1166	the enzymatic cleavage	1145:1166	the enzymatic cleavage of glycans	1145:1177	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	6	32	theme	pauci-mannosidic-type	1296:1316	arg1	glycans					1318:1324	pauci-mannosidic-type glycans	1296:1324	pauci-mannosidic-type glycans	1296:1324	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	1	33	theme	Golgi-localized	259:273	arg1	enzyme					297:302	a Golgi-localized type II transmembrane enzyme	257:302	a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans	257:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	33	theme	Golgi-localized	259:273	arg1	step					418:421	an essential step	405:421	an essential step in the conversion of oligomannose-type to complex-type N-glycans	405:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	33	theme	Golgi-localized	259:273	arg1	β-1,2-N-Acetylglucosaminyltransferase					191:227	β-1,2-N-Acetylglucosaminyltransferase II	191:230	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143)	191:252	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	6	34	theme	enzymatic	1471:1479	arg1	activity					1481:1488	the enzymatic activity	1467:1488	the enzymatic activity	1467:1488	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	2	35	theme	structure-function	597:614	arg1	relationship					616:627	structure-function relationship	597:627	structure-function relationship	597:627	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	1	36	theme	oligomannose-type	444:460	arg1	N-glycans					478:486	oligomannose-type to complex-type N-glycans	444:486	oligomannose-type to complex-type N-glycans	444:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	3	37	theme	silkworm-based	668:681	arg1	nucleopolyhedrovirus					695:714	silkworm-based Bombyx mori nucleopolyhedrovirus	668:714	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	1	38	theme	type	275:278	arg1	enzyme					297:302	a Golgi-localized type II transmembrane enzyme	257:302	a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans	257:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	38	theme	type	275:278	arg1	step					418:421	an essential step	405:421	an essential step in the conversion of oligomannose-type to complex-type N-glycans	405:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	38	theme	type	275:278	arg1	β-1,2-N-Acetylglucosaminyltransferase					191:227	β-1,2-N-Acetylglucosaminyltransferase II	191:230	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143)	191:252	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	0	39	theme	human	62:66	arg1	β-1,2-N-acetylglucosaminyltransferase					68:104	human β-1,2-N-acetylglucosaminyltransferase II	62:107	human β-1,2-N-acetylglucosaminyltransferase II	62:107	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	4	40	theme	glycosyltransferase	1037:1055	arg1	activity					1057:1064	glycosyltransferase activity	1037:1064	glycosyltransferase activity	1037:1064	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	2	41	from	reports	554:560	arg1	expression					582:591	heterologous expression	569:591	heterologous expression	569:591	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	2	41	from	reports	554:560	arg1	relationship					616:627	structure-function relationship	597:627	structure-function relationship	597:627	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	5	42	theme	insect	1206:1211	arg1	cells					1213:1217	insect cells	1206:1217	insect cells	1206:1217	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	2	43	dep	expression	582:591	arg1	the					565:567	the	565:567	the	565:567	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	1	44	theme	trimanosyl	375:384	arg1	core					386:389	the trimanosyl core	371:389	the trimanosyl core of N-glycans	371:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	45	theme	complex-type	465:476	arg1	N-glycans					478:486	oligomannose-type to complex-type N-glycans	444:486	oligomannose-type to complex-type N-glycans	444:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	4	46	theme	strict	1070:1075	arg1	specificity					1087:1097	strict substrate specificity	1070:1097	strict substrate specificity	1070:1097	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	3	47	theme	expression	723:732	arg1	system					734:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	1	48	theme	core	386:389	arg1	6-arm					362:366	the 6-arm	358:366	the 6-arm of the trimanosyl core of N-glycans	358:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	48	theme	core	386:389	arg1	core					386:389	the trimanosyl core	371:389	the trimanosyl core of N-glycans	371:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	6	49	theme	complex	1407:1413	arg1	type					1415:1418	the complex type	1403:1418	the complex type	1403:1418	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	1	50	theme	N-glycans	478:486	arg1	conversion					430:439	the conversion	426:439	the conversion of oligomannose-type to complex-type N-glycans	426:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	3	51	theme	nucleopolyhedrovirus	695:714	arg1	system					734:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	6	52	theme	N-glycans	1344:1352	arg1	structure					1331:1339	the structure	1327:1339	the structure of N-glycans in the recombinant hGnTII	1327:1378	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	6	53	from	structure	1331:1339	arg1	hGnTII					1373:1378	the recombinant hGnTII	1357:1378	the recombinant hGnTII	1357:1378	Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity.					
29409697	2	54	theme	enzyme	637:642	arg1	expression					582:591	heterologous expression	569:591	heterologous expression	569:591	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	2	54	theme	enzyme	637:642	arg1	relationship					616:627	structure-function relationship	597:627	structure-function relationship	597:627	Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme.					
29409697	3	55	theme	human	755:759	arg1	GnTII					761:765	human GnTII	755:765	human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region	755:837	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	3	55	theme	human	755:759	arg1	hGnTII					768:773	hGnTII	768:773	hGnTII	768:773	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	3	56	theme	bacmid	716:721	arg1	system					734:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	1	57	theme	transmembrane	283:295	arg1	enzyme					297:302	a Golgi-localized type II transmembrane enzyme	257:302	a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans	257:402	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	57	theme	transmembrane	283:295	arg1	step					418:421	an essential step	405:421	an essential step in the conversion of oligomannose-type to complex-type N-glycans	405:486	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	1	57	theme	transmembrane	283:295	arg1	β-1,2-N-Acetylglucosaminyltransferase					191:227	β-1,2-N-Acetylglucosaminyltransferase II	191:230	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143)	191:252	β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans.					
29409697	4	58	theme	substrate	1077:1085	arg1	specificity					1087:1097	strict substrate specificity	1070:1097	strict substrate specificity	1070:1097	The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity.					
29409697	3	59	theme	Bombyx	683:688	arg1	nucleopolyhedrovirus					695:714	silkworm-based Bombyx mori nucleopolyhedrovirus	668:714	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
29409697	5	60	gly	N-glycosylated	1127:1140	arg1	N-glycosylated					1127:1140	N-glycosylated	1127:1140	N-glycosylated	1127:1140	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	5	60	gly	N-glycosylated	1127:1140	arg1	enzyme					1104:1109	The enzyme	1100:1109	The enzyme	1100:1109	The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated.					
29409697	0	61	theme	β-1,2-N-acetylglucosaminyltransferase	68:104	arg1	characterization					42:57	characterization	42:57	characterization	42:57	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	0	61	theme	β-1,2-N-acetylglucosaminyltransferase	68:104	arg1	purification					25:36	purification	25:36	purification	25:36	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	0	61	theme	β-1,2-N-acetylglucosaminyltransferase	68:104	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression	0:22	Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.					
29409697	3	62	theme	mori	690:693	arg1	nucleopolyhedrovirus					695:714	silkworm-based Bombyx mori nucleopolyhedrovirus	668:714	a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system	666:739	Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region.					
30592377	2	0	theme	high	653:656	arg1	MALDI-IMS					678:686	high resolution N-glycan MALDI-IMS	653:686	high resolution N-glycan MALDI-IMS	653:686	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	7	1	theme	metastatic	1300:1309	arg1	tissues					1311:1317	paired primary and metastatic tissues	1281:1317	paired primary and metastatic tissues	1281:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	3	2	theme	metastatic	737:746	arg1	tumors					755:760	primary and metastatic breast tumors	725:760	primary and metastatic breast tumors from the same donors	725:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	4	3	theme	glycan	931:936	arg1	classes					949:955	specific glycan structural classes	922:955	specific glycan structural classes	922:955	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	4	4	theme	classes	949:955	arg1	distribution					906:917	the distribution	902:917	the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions	902:995	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	1	5	theme	tissue	312:317	arg1	sections					319:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	6	theme	spectrometry	219:230	arg1	method					244:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	6	theme	spectrometry	219:230	arg1	cancer					265:270	human breast cancer	252:270	human breast cancer	252:270	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	2	7	theme	N-glycan	669:676	arg1	MALDI-IMS					678:686	high resolution N-glycan MALDI-IMS	653:686	high resolution N-glycan MALDI-IMS	653:686	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	6	8	theme	cancer	1216:1221	arg1	tissues					1223:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	6	9	theme	metastatic	1198:1207	arg1	cancer					1216:1221	metastatic breast cancer	1198:1221	metastatic breast cancer	1198:1221	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	1	10	dep	paraffin-embedded	287:303	arg1	FFPE					306:309	FFPE	306:309	FFPE	306:309	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	11	from	distribution	391:402	arg1	microenvironment					417:432	the tumor microenvironment	407:432	the tumor microenvironment	407:432	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	12	theme	human	252:256	arg1	method					244:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	12	theme	human	252:256	arg1	cancer					265:270	human breast cancer	252:270	human breast cancer	252:270	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	6	13	located	detected	1160:1167	arg2	series					1122:1127	a series	1120:1127	a series of polylactosamine glycans	1120:1154	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	6	13	located	detected	1160:1167	arg1	tissues					1223:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	2	14	theme	FFPE	617:620	arg1	format					628:633	FFPE slide format	617:633	FFPE slide format	617:633	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	1	15	theme	developed	152:160	arg1	method					244:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	15	theme	developed	152:160	arg1	cancer					265:270	human breast cancer	252:270	human breast cancer	252:270	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	2	16	theme	triple-negative	563:577	arg1	cancers					586:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers	484:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC)	484:599	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	2	16	theme	triple-negative	563:577	arg1	TNBC					595:598	TNBC	595:598	TNBC	595:598	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	8	17	theme	cancers	1596:1602	arg1	progression					1574:1584	progression	1574:1584	progression	1574:1584	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	8	17	theme	cancers	1596:1602	arg1	development					1558:1568	development	1558:1568	development	1558:1568	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	0	18	theme	HER2-Positive	70:82	arg1	Tissues					118:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	1	19	theme	laser	178:182	arg1	MALDI-IMS					233:241	MALDI-IMS	233:241	MALDI-IMS	233:241	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	19	theme	laser	178:182	arg1	spectrometry					219:230	matrix-assisted laser desorption/ionization imaging mass spectrometry	162:230	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	2	20	theme	receptor-positive	541:557	arg1	cancers					586:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers	484:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC)	484:599	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	2	20	theme	receptor-positive	541:557	arg1	TNBC					595:598	TNBC	595:598	TNBC	595:598	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	0	21	theme	Triple-Negative	88:102	arg1	Tissues					118:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	1	22	theme	N-linked	375:382	arg1	distribution					391:402	N-linked glycan distribution	375:402	N-linked glycan distribution in the tumor microenvironment	375:432	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	23	theme	imaging	206:212	arg1	MALDI-IMS					233:241	MALDI-IMS	233:241	MALDI-IMS	233:241	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	23	theme	imaging	206:212	arg1	spectrometry					219:230	matrix-assisted laser desorption/ionization imaging mass spectrometry	162:230	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	0	24	from	Increases	0:8	arg1	Polylactosamines					28:43	Tumor N-Glycan Polylactosamines	13:43	Tumor N-Glycan Polylactosamines	13:43	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	4	25	theme	cumulative	815:824	arg1	analysis					845:852	The cumulative N-glycan MALDI-IMS analysis	811:852	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs	803:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	0	26	theme	Cancer	111:116	arg1	Tissues					118:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	9	27	theme	therapeutic	1657:1667	arg1	strategies					1669:1678	therapeutic strategies	1657:1678	therapeutic strategies	1657:1678	The presence of these glycans could be targeted for therapeutic strategies and further evaluation as potential prognostic biomarkers.					
30592377	4	28	theme	MALDI-IMS	835:843	arg1	analysis					845:852	The cumulative N-glycan MALDI-IMS analysis	811:852	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs	803:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	2	29	theme	DESIGN	448:453	arg1	sections					462:469	EXPERIMENTAL DESIGN Tissue sections	435:469	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format	435:633	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	7	30	theme	N-glycan	1252:1259	arg1	species					1261:1267	tumor N-glycan species	1246:1267	tumor N-glycan species detected in paired primary and metastatic tissues	1246:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	8	31	from	prevalence	1411:1420	arg1	primary					1469:1475	primary	1469:1475	primary	1469:1475	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	8	31	from	prevalence	1411:1420	arg1	cancer					1499:1504	metastatic breast cancer	1481:1504	metastatic breast cancer	1481:1504	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	6	32	theme	polylactosamine	1132:1146	arg1	glycans					1148:1154	polylactosamine glycans	1132:1154	polylactosamine glycans	1132:1154	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	2	33	theme	factor	516:521	arg1	receptor					523:530	human epidermal growth factor receptor 2	493:532	human epidermal growth factor receptor 2	493:532	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	2	34	theme	epidermal	499:507	arg1	receptor					523:530	human epidermal growth factor receptor 2	493:532	human epidermal growth factor receptor 2	493:532	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	8	35	theme	metastatic	1481:1490	arg1	cancer					1499:1504	metastatic breast cancer	1481:1504	metastatic breast cancer	1481:1504	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	2	36	theme	multiple	484:491	arg1	cancers					586:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers	484:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC)	484:599	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	2	36	theme	multiple	484:491	arg1	TNBC					595:598	TNBC	595:598	TNBC	595:598	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	0	37	theme	Tumor	13:17	arg1	Polylactosamines					28:43	Tumor N-Glycan Polylactosamines	13:43	Tumor N-Glycan Polylactosamines	13:43	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	1	38	theme	tissue	332:337	arg1	TMA					352:354	TMA	352:354	TMA	352:354	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	38	theme	tissue	332:337	arg1	microarrays					339:349	tissue microarrays	332:349	tissue microarrays (TMA)	332:355	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	9	39	theme	potential	1706:1714	arg1	biomarkers					1727:1736	potential prognostic biomarkers	1706:1736	potential prognostic biomarkers	1706:1736	The presence of these glycans could be targeted for therapeutic strategies and further evaluation as potential prognostic biomarkers.					
30592377	9	40	theme	glycans	1627:1633	arg1	presence					1609:1616	The presence	1605:1616	The presence of these glycans	1605:1633	The presence of these glycans could be targeted for therapeutic strategies and further evaluation as potential prognostic biomarkers.					
30592377	3	41	theme	tissue	708:713	arg1	cohort					715:720	An additional FFPE tissue cohort	689:720	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors	689:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	1	42	theme	mass	214:217	arg1	MALDI-IMS					233:241	MALDI-IMS	233:241	MALDI-IMS	233:241	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	42	theme	mass	214:217	arg1	spectrometry					219:230	matrix-assisted laser desorption/ionization imaging mass spectrometry	162:230	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	3	43	theme	additional	692:701	arg1	cohort					715:720	An additional FFPE tissue cohort	689:720	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors	689:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	8	44	dep	development	1558:1568	arg1	the					1554:1556	the	1554:1556	the	1554:1556	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	6	45	theme	TNBC	1188:1191	arg1	tissues					1223:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	8	46	theme	mechanistic	1528:1538	arg1	insights					1540:1547	new mechanistic insights	1524:1547	new mechanistic insights into the development and progression of breast cancers	1524:1602	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	8	47	theme	breast	1492:1497	arg1	cancer					1499:1504	metastatic breast cancer	1481:1504	metastatic breast cancer	1481:1504	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	1	48	theme	breast	258:263	arg1	method					244:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	48	theme	breast	258:263	arg1	cancer					265:270	human breast cancer	252:270	human breast cancer	252:270	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	5	49	theme	glycans	1049:1055	arg1	series					1000:1005	A series	998:1005	A series of high-mannose, branched and fucosylated glycans	998:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	6	50	theme	HER2+	1181:1185	arg1	tissues					1223:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	3	51	from	cohort	715:720	arg1	donors					776:781	the same donors	767:781	the same donors	767:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	4	52	theme	cancer	864:869	arg1	analysis					845:852	The cumulative N-glycan MALDI-IMS analysis	811:852	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs	803:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	1	53	theme	formalin-fixed	272:285	arg1	sections					319:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	7	54	theme	minimal	1328:1334	arg1	changes					1336:1342	minimal changes	1328:1342	minimal changes between the two conditions	1328:1369	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	4	55	theme	specific	922:929	arg1	classes					949:955	specific glycan structural classes	922:955	specific glycan structural classes	922:955	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	3	56	theme	breast	748:753	arg1	tumors					755:760	primary and metastatic breast tumors	725:760	primary and metastatic breast tumors from the same donors	725:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	5	57	located	detected	1061:1068	arg2	series					1000:1005	A series	998:1005	A series of high-mannose, branched and fucosylated glycans	998:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	5	57	located	detected	1061:1068	arg1	regions					1097:1103	tumor regions	1091:1103	tumor regions	1091:1103	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	4	58	theme	structural	938:947	arg1	classes					949:955	specific glycan structural classes	922:955	specific glycan structural classes	922:955	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	4	59	dep	cancer	864:869	arg1	tissues					876:882	FFPE tissues	871:882	FFPE tissues	871:882	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	4	59	dep	cancer	864:869	arg1	TMAs					888:891	TMAs	888:891	TMAs	888:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	3	60	theme	same	771:774	arg1	donors					776:781	the same donors	767:781	the same donors	767:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	7	61	theme	paired	1281:1286	arg1	tissues					1311:1317	paired primary and metastatic tissues	1281:1317	paired primary and metastatic tissues	1281:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	8	62	dep	primary	1469:1475	arg1	tissues					1506:1512	tissues	1506:1512	tissues	1506:1512	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	2	63	theme	resolution	658:667	arg1	MALDI-IMS					678:686	high resolution N-glycan MALDI-IMS	653:686	high resolution N-glycan MALDI-IMS	653:686	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	6	64	theme	breast	1209:1214	arg1	cancer					1216:1221	metastatic breast cancer	1198:1221	metastatic breast cancer	1198:1221	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	3	65	from	donors	776:781	arg1	tumors					755:760	primary and metastatic breast tumors	725:760	primary and metastatic breast tumors from the same donors	725:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	3	65	from	donors	776:781	arg1	cohort					715:720	An additional FFPE tissue cohort	689:720	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors	689:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	4	66	theme	tumor	983:987	arg1	regions					989:995	tumor regions	983:995	tumor regions	983:995	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	4	67	dep	RESULTS	803:809	arg1	analysis					845:852	The cumulative N-glycan MALDI-IMS analysis	811:852	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs	803:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	2	68	theme	slide	622:626	arg1	format					628:633	FFPE slide format	617:633	FFPE slide format	617:633	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	8	69	theme	breast	1589:1594	arg1	cancers					1596:1602	breast cancers	1589:1602	breast cancers	1589:1602	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	8	70	theme	tumor-associated	1425:1440	arg1	glycans					1458:1464	tumor-associated polylactosamine glycans	1425:1464	tumor-associated polylactosamine glycans	1425:1464	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	7	71	theme	primary	1288:1294	arg1	tissues					1311:1317	paired primary and metastatic tissues	1281:1317	paired primary and metastatic tissues	1281:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	1	72	theme	matrix-assisted	162:176	arg1	MALDI-IMS					233:241	MALDI-IMS	233:241	MALDI-IMS	233:241	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	72	theme	matrix-assisted	162:176	arg1	spectrometry					219:230	matrix-assisted laser desorption/ionization imaging mass spectrometry	162:230	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	8	73	theme	CLINICAL	1388:1395	arg1	RELEVANCE					1397:1405	CLINICAL RELEVANCE	1388:1405	CLINICAL RELEVANCE	1388:1405	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	0	74	theme	Breast	104:109	arg1	Tissues					118:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	1	75	theme	desorption/ionization	184:204	arg1	MALDI-IMS					233:241	MALDI-IMS	233:241	MALDI-IMS	233:241	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	1	75	theme	desorption/ionization	184:204	arg1	spectrometry					219:230	matrix-assisted laser desorption/ionization imaging mass spectrometry	162:230	a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method	141:249	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	7	76	theme	species	1261:1267	arg1	Comparison					1232:1241	Comparison	1232:1241	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues	1232:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	1	77	theme	glycan	384:389	arg1	distribution					391:402	N-linked glycan distribution	375:402	N-linked glycan distribution in the tumor microenvironment	375:432	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	5	78	theme	high-mannose	1010:1021	arg1	glycans					1049:1055	high-mannose, branched and fucosylated glycans	1010:1055	high-mannose, branched and fucosylated glycans	1010:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	7	79	theme	tumor	1246:1250	arg1	species					1261:1267	tumor N-glycan species	1246:1267	tumor N-glycan species detected in paired primary and metastatic tissues	1246:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	5	80	theme	branched	1024:1031	arg1	glycans					1049:1055	high-mannose, branched and fucosylated glycans	1010:1055	high-mannose, branched and fucosylated glycans	1010:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	6	81	theme	glycans	1148:1154	arg1	series					1122:1127	a series	1120:1127	a series of polylactosamine glycans	1120:1154	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	2	82	theme	breast	579:584	arg1	cancers					586:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers	484:592	multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC)	484:599	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	2	82	theme	breast	579:584	arg1	TNBC					595:598	TNBC	595:598	TNBC	595:598	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	4	83	theme	N-glycan	826:833	arg1	analysis					845:852	The cumulative N-glycan MALDI-IMS analysis	811:852	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs	803:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	2	84	theme	EXPERIMENTAL	435:446	arg1	sections					462:469	EXPERIMENTAL DESIGN Tissue sections	435:469	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format	435:633	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	1	85	theme	tumor	411:415	arg1	microenvironment					417:432	the tumor microenvironment	407:432	the tumor microenvironment	407:432	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	9	86	theme	further	1684:1690	arg1	evaluation					1692:1701	further evaluation	1684:1701	further evaluation	1684:1701	The presence of these glycans could be targeted for therapeutic strategies and further evaluation as potential prognostic biomarkers.					
30592377	2	87	theme	growth	509:514	arg1	receptor					523:530	human epidermal growth factor receptor 2	493:532	human epidermal growth factor receptor 2	493:532	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	1	88	link	N-linked	375:382	arg1	distribution					391:402	N-linked glycan distribution	375:402	N-linked glycan distribution in the tumor microenvironment	375:432	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	0	89	theme	N-Glycan	19:26	arg1	Polylactosamines					28:43	Tumor N-Glycan Polylactosamines	13:43	Tumor N-Glycan Polylactosamines	13:43	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	2	90	theme	human	493:497	arg1	receptor					523:530	human epidermal growth factor receptor 2	493:532	human epidermal growth factor receptor 2	493:532	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	8	91	theme	glycans	1458:1464	arg1	prevalence					1411:1420	The prevalence	1407:1420	The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues	1407:1512	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	3	92	theme	FFPE	703:706	arg1	cohort					715:720	An additional FFPE tissue cohort	689:720	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors	689:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	8	93	dep	CONCLUSIONS	1372:1382	arg1	prevalence					1411:1420	The prevalence	1407:1420	The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues	1407:1512	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	9	94	theme	prognostic	1716:1725	arg1	biomarkers					1727:1736	potential prognostic biomarkers	1706:1736	potential prognostic biomarkers	1706:1736	The presence of these glycans could be targeted for therapeutic strategies and further evaluation as potential prognostic biomarkers.					
30592377	5	95	gly	fucosylated	1037:1047	arg1	glycans					1049:1055	high-mannose, branched and fucosylated glycans	1010:1055	high-mannose, branched and fucosylated glycans	1010:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	4	96	theme	FFPE	871:874	arg1	tissues					876:882	FFPE tissues	871:882	FFPE tissues	871:882	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	7	97	located	detected	1269:1276	arg1	tissues					1311:1317	paired primary and metastatic tissues	1281:1317	paired primary and metastatic tissues	1281:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	7	97	located	detected	1269:1276	arg2	species					1261:1267	tumor N-glycan species	1246:1267	tumor N-glycan species detected in paired primary and metastatic tissues	1246:1317	Comparison of tumor N-glycan species detected in paired primary and metastatic tissues indicate minimal changes between the two conditions.					
30592377	2	98	theme	Tissue	455:460	arg1	sections					462:469	EXPERIMENTAL DESIGN Tissue sections	435:469	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format	435:633	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	0	99	theme	Advanced	61:68	arg1	Tissues					118:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues	61:124	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.					
30592377	2	100	dep	receptor-positive	541:557	arg1	receptor					523:530	human epidermal growth factor receptor 2	493:532	human epidermal growth factor receptor 2	493:532	EXPERIMENTAL DESIGN Tissue sections representing multiple human epidermal growth factor receptor 2 (HER2) receptor-positive and triple-negative breast cancers (TNBC) in both TMA and FFPE slide format are processed for high resolution N-glycan MALDI-IMS.					
30592377	5	101	theme	tumor	1091:1095	arg1	regions					1097:1103	tumor regions	1091:1103	tumor regions	1091:1103	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	3	102	theme	primary	725:731	arg1	tumors					755:760	primary and metastatic breast tumors	725:760	primary and metastatic breast tumors from the same donors	725:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	1	103	dep	method	244:249	arg1	sections					319:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
30592377	5	104	theme	fucosylated	1037:1047	arg1	glycans					1049:1055	high-mannose, branched and fucosylated glycans	1010:1055	high-mannose, branched and fucosylated glycans	1010:1055	A series of high-mannose, branched and fucosylated glycans are detected predominantly within tumor regions.					
30592377	8	105	theme	polylactosamine	1442:1456	arg1	glycans					1458:1464	tumor-associated polylactosamine glycans	1425:1464	tumor-associated polylactosamine glycans	1425:1464	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	8	106	theme	new	1524:1526	arg1	insights					1540:1547	new mechanistic insights	1524:1547	new mechanistic insights into the development and progression of breast cancers	1524:1602	CONCLUSIONS AND CLINICAL RELEVANCE The prevalence of tumor-associated polylactosamine glycans in primary and metastatic breast cancer tissues indicates new mechanistic insights into the development and progression of breast cancers.					
30592377	6	107	theme	advanced	1172:1179	arg1	tissues					1223:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	advanced HER2+, TNBC, and metastatic breast cancer tissues	1172:1229	Additionally, a series of polylactosamine glycans are detected in advanced HER2+, TNBC, and metastatic breast cancer tissues.					
30592377	3	108	theme	tumors	755:760	arg1	cohort					715:720	An additional FFPE tissue cohort	689:720	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors	689:781	An additional FFPE tissue cohort of primary and metastatic breast tumors from the same donors are also evaluated.					
30592377	4	109	theme	breast	857:862	arg1	cancer					864:869	breast cancer FFPE tissues and TMAs	857:891	breast cancer FFPE tissues and TMAs	857:891	RESULTS The cumulative N-glycan MALDI-IMS analysis of breast cancer FFPE tissues and TMAs indicate the distribution of specific glycan structural classes to stromal, necrotic, and tumor regions.					
30592377	1	110	theme	paraffin-embedded	287:303	arg1	sections					319:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	formalin-fixed paraffin-embedded (FFPE) tissue sections	272:326	PURPOSE Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) are evaluated for N-linked glycan distribution in the tumor microenvironment.					
31279885	0	0	theme	solid-state	87:97	arg1	fermentation					99:110	solid-state fermentation	87:110	solid-state fermentation	87:110	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	3	1	theme	RAW	867:869	arg1	cells					888:892	mouse RAW 264.7 macrophage cells	861:892	mouse RAW 264.7 macrophage cells	861:892	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	2	2	theme	1 → 6	729:733	arg1	-Manp					749:753	(1 → 3)-Manp	742:753	(1 → 3)-Manp	742:753	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	2	theme	1 → 6	729:733	arg1	-Glcp					735:739	(1 → 6)-Glcp	728:739	(1 → 6)-Glcp	728:739	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	3	theme	1 → 6	760:764	arg1	-Galp					766:770	(1 → 6)-Galp	759:770	(1 → 6)-Galp	759:770	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	4	theme	1 → 2,6	516:522	arg1	-Manp					524:528	(1 → 2,6)-Manp	515:528	(1 → 2,6)-Manp	515:528	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	3	5	theme	mouse	861:865	arg1	cells					888:892	mouse RAW 264.7 macrophage cells	861:892	mouse RAW 264.7 macrophage cells	861:892	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	2	6	theme	1 → 5	701:705	arg1	-Glcp					721:725	(1 → 4)-Glcp	714:725	(1 → 4)-Glcp	714:725	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	6	theme	1 → 5	701:705	arg1	-Araf					707:711	(1 → 5)-Araf	700:711	(1 → 5)-Araf	700:711	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	0	7	from	Structure	0:8	arg1	solani					73:78	solani	73:78	solani	73:78	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	2	8	theme	glycosidic	547:556	arg1	linkages					558:565	(1 → 6)-Galp glycosidic linkages	534:565	(1 → 6)-Galp glycosidic linkages	534:565	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	6	9	theme	immunomodulatory	1410:1425	arg1	activity					1427:1434	immunomodulatory activity	1410:1434	immunomodulatory activity	1410:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	2	10	contain	contained	690:698	arg2	-Araf					707:711	(1 → 5)-Araf	700:711	(1 → 5)-Araf	700:711	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	10	contain	contained	690:698	arg2	-Glcp					721:725	(1 → 4)-Glcp	714:725	(1 → 4)-Glcp	714:725	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	10	contain	contained	690:698	arg2	-Galp					766:770	(1 → 6)-Galp	759:770	(1 → 6)-Galp	759:770	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	10	contain	contained	690:698	arg1	DGS1					333:336	DGS1	333:336	DGS1	333:336	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	10	contain	contained	690:698	arg2	-Manp					749:753	(1 → 3)-Manp	742:753	(1 → 3)-Manp	742:753	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	10	contain	contained	690:698	arg2	-Glcp					735:739	(1 → 6)-Glcp	728:739	(1 → 6)-Glcp	728:739	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	11	theme	-Galp	541:545	arg1	linkages					558:565	(1 → 6)-Galp glycosidic linkages	534:565	(1 → 6)-Galp glycosidic linkages	534:565	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	12	theme	molar	642:646	arg1	ratio					648:652	a molar ratio	640:652	a molar ratio of 3.5:8.1:2.1:1	640:669	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	6	13	theme	products	1361:1368	arg1	source					1343:1348	a commercially competitive source	1316:1348	a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity	1316:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	13	theme	products	1361:1368	arg1	potential					1303:1311	significant potential	1291:1311	significant potential	1291:1311	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	14	theme	fungal	1381:1386	arg1	polysaccharides					1388:1402	fungal polysaccharides	1381:1402	fungal polysaccharides	1381:1402	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	2	15	theme	1 → 6	535:539	arg1	linkages					558:565	(1 → 6)-Galp glycosidic linkages	534:565	(1 → 6)-Galp glycosidic linkages	534:565	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	5	16	contain	possessed	1135:1143	arg1	DGS2					1125:1128	DGS2	1125:1128	DGS2	1125:1128	Interestingly, DGS2 also possessed relatively high antioxidant activity.					
31279885	5	16	contain	possessed	1135:1143	arg2	activity					1173:1180	relatively high antioxidant activity	1145:1180	relatively high antioxidant activity	1145:1180	Interestingly, DGS2 also possessed relatively high antioxidant activity.					
31279885	1	17	theme	solid-state	166:176	arg1	SSF					192:194	SSF	192:194	SSF	192:194	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	17	theme	solid-state	166:176	arg1	fermentation					178:189	solid-state fermentation	166:189	solid-state fermentation (SSF) of Fusarium solani DO7	166:218	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	17	theme	solid-state	166:176	arg1	fungus					235:240	an endophytic fungus	221:240	an endophytic fungus isolated from the orchid Dendrobium officinale	221:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	6	18	theme	cost-effectiveness	1225:1242	arg1	features					1273:1280	its cost-effectiveness and environmentally friendly features	1221:1280	its cost-effectiveness and environmentally friendly features	1221:1280	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	2	19	contain	contained	449:457	arg2	-Araf					466:470	(1 → 5)-Araf	459:470	(1 → 5)-Araf	459:470	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg2	-Glcp					480:484	(1 → 4)-Glcp	473:484	(1 → 4)-Glcp	473:484	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg2	-Glcp					494:498	(1 → 6)-Glcp	487:498	(1 → 6)-Glcp	487:498	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg1	DGS1					333:336	DGS1	333:336	DGS1	333:336	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg2	-Manp					508:512	(1 → 3)-Manp	501:512	(1 → 3)-Manp	501:512	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg2	linkages					558:565	(1 → 6)-Galp glycosidic linkages	534:565	(1 → 6)-Galp glycosidic linkages	534:565	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	19	contain	contained	449:457	arg2	-Manp					524:528	(1 → 2,6)-Manp	515:528	(1 → 2,6)-Manp	515:528	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	20	theme	glycosidic	772:781	arg1	linkages					783:790	glycosidic linkages	772:790	glycosidic linkages	772:790	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	3	21	theme	macrophage	877:886	arg1	cells					888:892	mouse RAW 264.7 macrophage cells	861:892	mouse RAW 264.7 macrophage cells	861:892	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	2	22	dep	-Araf	707:711	arg1	linkages					783:790	glycosidic linkages	772:790	glycosidic linkages	772:790	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	5	23	theme	high	1156:1159	arg1	activity					1173:1180	relatively high antioxidant activity	1145:1180	relatively high antioxidant activity	1145:1180	Interestingly, DGS2 also possessed relatively high antioxidant activity.					
31279885	6	24	theme	friendly	1264:1271	arg1	features					1273:1280	its cost-effectiveness and environmentally friendly features	1221:1280	its cost-effectiveness and environmentally friendly features	1221:1280	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	1	25	theme	orchid	260:265	arg1	officinale					278:287	the orchid Dendrobium officinale	256:287	the orchid Dendrobium officinale	256:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	3	26	theme	human	829:833	arg1	cells					852:856	human embryonic kidney cells	829:856	human embryonic kidney cells	829:856	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	4	27	theme	activity	915:922	arg1	assay					924:928	An immunomodulatory activity assay	895:928	An immunomodulatory activity assay	895:928	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	6	28	theme	environmentally	1248:1262	arg1	features					1273:1280	its cost-effectiveness and environmentally friendly features	1221:1280	its cost-effectiveness and environmentally friendly features	1221:1280	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	1	29	theme	Dendrobium	267:276	arg1	officinale					278:287	the orchid Dendrobium officinale	256:287	the orchid Dendrobium officinale	256:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	3	30	theme	embryonic	835:843	arg1	cells					852:856	human embryonic kidney cells	829:856	human embryonic kidney cells	829:856	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	2	31	theme	3.5:8.1:2.1:1	657:669	arg1	ratio					648:652	a molar ratio	640:652	a molar ratio of 3.5:8.1:2.1:1	640:669	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	32	theme	2.9:13.4:3.0:1	415:428	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 2.9:13.4:3.0:1	398:428	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	0	33	theme	immunomodulatory	14:29	arg1	activity					31:38	immunomodulatory activity	14:38	immunomodulatory activity	14:38	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	2	34	theme	Structural	290:299	arg1	characterizations					301:317	Structural characterizations	290:317	Structural characterizations	290:317	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	6	35	theme	natural	1353:1359	arg1	polysaccharides					1388:1402	fungal polysaccharides	1381:1402	fungal polysaccharides	1381:1402	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	35	theme	natural	1353:1359	arg1	products					1361:1368	natural products	1353:1368	natural products	1353:1368	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	0	36	theme	polysaccharides	43:57	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	0	36	theme	polysaccharides	43:57	arg1	activity					31:38	immunomodulatory activity	14:38	immunomodulatory activity	14:38	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	4	37	theme	NO	1024:1025	arg1	levels					998:1003	the levels	994:1003	the levels of TNF-α, IL-6 and NO	994:1025	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	6	38	theme	competitive	1331:1341	arg1	source					1343:1348	a commercially competitive source	1316:1348	a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity	1316:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	38	theme	competitive	1331:1341	arg1	potential					1303:1311	significant potential	1291:1311	significant potential	1291:1311	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	2	39	theme	1 → 5	460:464	arg1	-Glcp					480:484	(1 → 4)-Glcp	473:484	(1 → 4)-Glcp	473:484	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	39	theme	1 → 5	460:464	arg1	-Araf					466:470	(1 → 5)-Araf	459:470	(1 → 5)-Araf	459:470	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	40	theme	1 → 6	488:492	arg1	-Glcp					494:498	(1 → 6)-Glcp	487:498	(1 → 6)-Glcp	487:498	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	6	41	with	polysaccharides	1388:1402	arg1	activity					1427:1434	immunomodulatory activity	1410:1434	immunomodulatory activity	1410:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	2	42	from	galactose	627:635	arg1	ratio					648:652	a molar ratio	640:652	a molar ratio of 3.5:8.1:2.1:1	640:669	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	4	43	theme	TNF-α	1008:1012	arg1	levels					998:1003	the levels	994:1003	the levels of TNF-α, IL-6 and NO	994:1025	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	1	44	theme	solani	209:214	arg1	SSF					192:194	SSF	192:194	SSF	192:194	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	44	theme	solani	209:214	arg1	fermentation					178:189	solid-state fermentation	166:189	solid-state fermentation (SSF) of Fusarium solani DO7	166:218	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	44	theme	solani	209:214	arg1	fungus					235:240	an endophytic fungus	221:240	an endophytic fungus isolated from the orchid Dendrobium officinale	221:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	0	45	from	solani	73:78	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Fusarium solani	43:78	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	0	45	from	solani	73:78	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	0	45	from	solani	73:78	arg1	activity					31:38	immunomodulatory activity	14:38	immunomodulatory activity	14:38	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	6	46	contain	has	1287:1289	arg1	SSF					1283:1285	SSF	1283:1285	SSF	1283:1285	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	46	contain	has	1287:1289	arg2	source					1343:1348	a commercially competitive source	1316:1348	a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity	1316:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	46	contain	has	1287:1289	arg2	potential					1303:1311	significant potential	1291:1311	significant potential	1291:1311	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	4	47	theme	gene	1062:1065	arg1	expression					1067:1076	iNOs gene expression	1057:1076	iNOs gene expression	1057:1076	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	4	48	theme	iNOs	1057:1060	arg1	expression					1067:1076	iNOs gene expression	1057:1076	iNOs gene expression	1057:1076	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	4	49	theme	IL-6	1015:1018	arg1	levels					998:1003	the levels	994:1003	the levels of TNF-α, IL-6 and NO	994:1025	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	2	50	theme	1 → 3	743:747	arg1	-Manp					749:753	(1 → 3)-Manp	742:753	(1 → 3)-Manp	742:753	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	50	theme	1 → 3	743:747	arg1	-Glcp					735:739	(1 → 6)-Glcp	728:739	(1 → 6)-Glcp	728:739	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	51	theme	1 → 3	502:506	arg1	-Manp					508:512	(1 → 3)-Manp	501:512	(1 → 3)-Manp	501:512	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	3	52	theme	kidney	845:850	arg1	cells					852:856	human embryonic kidney cells	829:856	human embryonic kidney cells	829:856	Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells.					
31279885	1	53	attach	isolated	242:249	arg1	officinale					278:287	the orchid Dendrobium officinale	256:287	the orchid Dendrobium officinale	256:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	53	attach	isolated	242:249	arg2	fungus					235:240	an endophytic fungus	221:240	an endophytic fungus isolated from the orchid Dendrobium officinale	221:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	53	attach	isolated	242:249	arg2	fermentation					178:189	solid-state fermentation	166:189	solid-state fermentation (SSF) of Fusarium solani DO7	166:218	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	2	54	from	mannose	615:621	arg1	ratio					648:652	a molar ratio	640:652	a molar ratio of 3.5:8.1:2.1:1	640:669	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	5	55	theme	antioxidant	1161:1171	arg1	activity					1173:1180	relatively high antioxidant activity	1145:1180	relatively high antioxidant activity	1145:1180	Interestingly, DGS2 also possessed relatively high antioxidant activity.					
31279885	4	56	theme	immunomodulatory	898:913	arg1	assay					924:928	An immunomodulatory activity assay	895:928	An immunomodulatory activity assay	895:928	An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2.					
31279885	2	57	theme	1 → 4	715:719	arg1	-Glcp					721:725	(1 → 4)-Glcp	714:725	(1 → 4)-Glcp	714:725	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	57	theme	1 → 4	715:719	arg1	-Araf					707:711	(1 → 5)-Araf	700:711	(1 → 5)-Araf	700:711	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	58	theme	1 → 4	474:478	arg1	-Glcp					480:484	(1 → 4)-Glcp	473:484	(1 → 4)-Glcp	473:484	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	58	theme	1 → 4	474:478	arg1	-Araf					466:470	(1 → 5)-Araf	459:470	(1 → 5)-Araf	459:470	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	2	59	theme	molar	400:404	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 2.9:13.4:3.0:1	398:428	Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages.					
31279885	0	60	from	activity	31:38	arg1	solani					73:78	solani	73:78	solani	73:78	Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.					
31279885	6	61	theme	significant	1291:1301	arg1	source					1343:1348	a commercially competitive source	1316:1348	a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity	1316:1434	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	6	61	theme	significant	1291:1301	arg1	potential					1303:1311	significant potential	1291:1311	significant potential	1291:1311	These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.					
31279885	1	62	theme	endophytic	224:233	arg1	fermentation					178:189	solid-state fermentation	166:189	solid-state fermentation (SSF) of Fusarium solani DO7	166:218	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
31279885	1	62	theme	endophytic	224:233	arg1	fungus					235:240	an endophytic fungus	221:240	an endophytic fungus isolated from the orchid Dendrobium officinale	221:287	Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale.					
29927116	7	0	theme	Flow	1271:1274	arg1	cytometry					1276:1284	Flow cytometry	1271:1284	Flow cytometry data	1271:1289	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	2	1	theme	cell	376:379	arg1	surface					381:387	the cell surface	372:387	the cell surface	372:387	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	1	2	theme	specific	252:259	arg1	structures					268:277	specific glycan structures	252:277	specific glycan structures	252:277	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	7	3	theme	cytometry	1276:1284	arg1	data					1286:1289	Flow cytometry data	1271:1289	Flow cytometry data	1271:1289	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	2	4	theme	glycans	355:361	arg1	heterogeneity					338:350	The complex chemical heterogeneity	317:350	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx	317:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	5	5	theme	multivalent	937:947	arg1	ligands					949:955	3D multivalent ligands	934:955	3D multivalent ligands projecting away from the cell surface	934:993	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	3	6	theme	glycans	536:542	arg1	study					516:520	the precise study	504:520	the precise study of individual glycans at different regions of the glycocalyx	504:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	1	7	theme	glycan	261:266	arg1	structures					268:277	specific glycan structures	252:277	specific glycan structures	252:277	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	6	8	theme	mimetic	1142:1148	arg1	incorporation					1150:1162	mimetic incorporation	1142:1162	mimetic incorporation	1142:1162	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	5	9	theme	2D	1082:1083	arg1	arrays					1085:1090	dynamic 2D arrays	1074:1090	dynamic 2D arrays proximal to the cell membrane	1074:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	10	theme	α-2,6-sialyllactose	1002:1020	arg1	conjugates					1033:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	1	11	theme	structures	268:277	arg1	display					241:247	cell surface display	228:247	cell surface display of specific glycan structures in predefined nanoscale arrangements	228:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	2	12	located	found	363:367	arg1	surface					381:387	the cell surface	372:387	the cell surface	372:387	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	2	12	located	found	363:367	arg1	glycocalyx					396:405	the glycocalyx	392:405	the glycocalyx	392:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	2	12	located	found	363:367	arg2	glycans					355:361	glycans	355:361	glycans found on the cell surface or the glycocalyx	355:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	4	13	dep	prepared	717:724	arg1	incubated					785:793	incubated	785:793	are incubated with the glycomaterials	781:817	CHO-Lec2 cells are prepared as adherent monolayers and, after reaching confluence, are incubated with the glycomaterials.					
29927116	7	14	theme	cell	1304:1307	arg1	remodeling					1317:1326	cell surface remodeling	1304:1326	cell surface remodeling with either glycoconjugate mimetic	1304:1361	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	3	15	theme	glycocalyx	572:581	arg1	regions					557:563	different regions	547:563	different regions of the glycocalyx	547:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	5	16	used	used	1048:1051	arg2	conjugates					1033:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	7	17	theme	surface	1309:1315	arg1	remodeling					1317:1326	cell surface remodeling	1304:1326	cell surface remodeling with either glycoconjugate mimetic	1304:1361	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	9	18	theme	John	1562:1565	arg1	Sons					1575:1578	John Wiley & Sons	1562:1578	John Wiley & Sons	1562:1578	© 2018 by John Wiley & Sons, Inc.					
29927116	5	19	from	glycans	1063:1069	arg1	arrays					1085:1090	dynamic 2D arrays	1074:1090	dynamic 2D arrays proximal to the cell membrane	1074:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	20	theme	ligands	949:955	arg1	form					926:929	the form	922:929	the form of 3D multivalent ligands projecting away from the cell surface	922:993	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	21	used	used	884:887	arg2	glycopolymers					830:842	Synthetic glycopolymers	820:842	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans	820:878	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	22	theme	dynamic	1074:1080	arg1	arrays					1085:1090	dynamic 2D arrays	1074:1090	dynamic 2D arrays proximal to the cell membrane	1074:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	3	23	theme	different	547:555	arg1	regions					557:563	different regions	547:563	different regions of the glycocalyx	547:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	1	24	with	cells	113:117	arg1	glycoprotein					134:145	synthetic glycoprotein	124:145	synthetic glycoprotein	124:145	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	1	24	with	cells	113:117	arg1	mimetics					162:169	glycolipid mimetics	151:169	glycolipid mimetics	151:169	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	8	25	with	glycocalyx	1498:1507	arg1	organization					1538:1549	organization	1538:1549	organization	1538:1549	Combinations of glycoconjugates can also be employed simultaneously to generate a mixed glycocalyx with tunable composition and organization.					
29927116	8	25	with	glycocalyx	1498:1507	arg1	composition					1522:1532	tunable composition	1514:1532	tunable composition	1514:1532	Combinations of glycoconjugates can also be employed simultaneously to generate a mixed glycocalyx with tunable composition and organization.					
29927116	5	26	theme	cell	982:985	arg1	surface					987:993	the cell surface	978:993	the cell surface	978:993	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	4	27	theme	CHO-Lec2	698:705	arg1	monolayers					738:747	adherent monolayers	729:747	adherent monolayers	729:747	CHO-Lec2 cells are prepared as adherent monolayers and, after reaching confluence, are incubated with the glycomaterials.					
29927116	4	27	theme	CHO-Lec2	698:705	arg1	cells					707:711	CHO-Lec2 cells	698:711	CHO-Lec2 cells	698:711	CHO-Lec2 cells are prepared as adherent monolayers and, after reaching confluence, are incubated with the glycomaterials.					
29927116	0	28	theme	Glycocalyx	0:9	arg1	Scaffolding					11:21	Glycocalyx Scaffolding	0:21	Glycocalyx Scaffolding to Control Cell Surface Glycan	0:52	Glycocalyx Scaffolding to Control Cell Surface Glycan Displays.					
29927116	1	29	theme	predefined	282:291	arg1	arrangements					303:314	predefined nanoscale arrangements	282:314	predefined nanoscale arrangements	282:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	3	30	from	study	516:520	arg1	regions					557:563	different regions	547:563	different regions of the glycocalyx	547:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	2	31	theme	chemical	329:336	arg1	heterogeneity					338:350	The complex chemical heterogeneity	317:350	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx	317:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	1	32	theme	lipid	200:204	arg1	anchors					206:212	lipid anchors	200:212	lipid anchors	200:212	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	0	33	theme	Control	26:32	arg1	Glycan					47:52	Control Cell Surface Glycan	26:52	Control Cell Surface Glycan	26:52	Glycocalyx Scaffolding to Control Cell Surface Glycan Displays.					
29927116	1	34	theme	nanoscale	293:301	arg1	arrangements					303:314	predefined nanoscale arrangements	282:314	predefined nanoscale arrangements	282:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	8	35	theme	mixed	1492:1496	arg1	glycocalyx					1498:1507	a mixed glycocalyx	1490:1507	a mixed glycocalyx with tunable composition and organization	1490:1549	Combinations of glycoconjugates can also be employed simultaneously to generate a mixed glycocalyx with tunable composition and organization.					
29927116	2	36	theme	complex	321:327	arg1	heterogeneity					338:350	The complex chemical heterogeneity	317:350	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx	317:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	2	37	theme	contributions	442:454	arg1	analysis					415:422	analysis	415:422	analysis of the individual contributions of glycans	415:465	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	6	38	theme	glycan	1168:1173	arg1	display					1175:1181	glycan display	1168:1181	glycan display	1168:1181	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	1	39	theme	remodeling	102:111	arg1	cells					113:117	remodeling cells	102:117	remodeling cells with synthetic glycoprotein and glycolipid mimetics	102:169	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	6	40	theme	sialic	1257:1262	arg1	acids					1264:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	2	41	theme	individual	431:440	arg1	contributions					442:454	the individual contributions	427:454	the individual contributions of glycans	427:465	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	0	42	theme	Surface	39:45	arg1	Glycan					47:52	Control Cell Surface Glycan	26:52	Control Cell Surface Glycan	26:52	Glycocalyx Scaffolding to Control Cell Surface Glycan Displays.					
29927116	1	43	gly	glycoprotein	134:145	arg1	glycoprotein					134:145	synthetic glycoprotein	124:145	synthetic glycoprotein	124:145	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	3	44	theme	stem	671:674	arg1	differentiation					681:695	stem cell differentiation	671:695	stem cell differentiation	671:695	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	9	45	theme	Wiley	1567:1571	arg1	Sons					1575:1578	John Wiley & Sons	1562:1578	John Wiley & Sons	1562:1578	© 2018 by John Wiley & Sons, Inc.					
29927116	0	46	theme	Cell	34:37	arg1	Glycan					47:52	Control Cell Surface Glycan	26:52	Control Cell Surface Glycan	26:52	Glycocalyx Scaffolding to Control Cell Surface Glycan Displays.					
29927116	7	47	with	remodeling	1317:1326	arg1	mimetic					1355:1361	mimetic	1355:1361	mimetic	1355:1361	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	2	48	theme	glycans	459:465	arg1	contributions					442:454	the individual contributions	427:454	the individual contributions of glycans	427:465	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	5	49	theme	cellular	901:908	arg1	surfaces					910:917	cellular surfaces	901:917	cellular surfaces	901:917	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	50	theme	Synthetic	820:828	arg1	glycopolymers					830:842	Synthetic glycopolymers	820:842	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans	820:878	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	3	51	theme	individual	525:534	arg1	glycans					536:542	individual glycans	525:542	individual glycans at different regions of the glycocalyx	525:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	1	52	from	display	241:247	arg1	arrangements					303:314	predefined nanoscale arrangements	282:314	predefined nanoscale arrangements	282:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	5	53	theme	glycolipid	1022:1031	arg1	conjugates					1033:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	α-2,6-sialyllactose glycolipid conjugates	1002:1042	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	6	54	theme	α-2,3-	1234:1239	arg1	acids					1264:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	5	55	theme	proximal	1092:1099	arg1	arrays					1085:1090	dynamic 2D arrays	1074:1090	dynamic 2D arrays proximal to the cell membrane	1074:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	6	56	link	α-2,6-linked	1244:1255	arg1	acids					1264:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	4	57	theme	adherent	729:736	arg1	monolayers					738:747	adherent monolayers	729:747	adherent monolayers	729:747	CHO-Lec2 cells are prepared as adherent monolayers and, after reaching confluence, are incubated with the glycomaterials.					
29927116	4	57	theme	adherent	729:736	arg1	cells					707:711	CHO-Lec2 cells	698:711	CHO-Lec2 cells	698:711	CHO-Lec2 cells are prepared as adherent monolayers and, after reaching confluence, are incubated with the glycomaterials.					
29927116	6	58	with	lectins	1205:1211	arg1	specificity					1218:1228	specificity	1218:1228	specificity for α-2,3- or α-2,6-linked sialic acids	1218:1268	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	8	59	theme	tunable	1514:1520	arg1	composition					1522:1532	tunable composition	1514:1532	tunable composition	1514:1532	Combinations of glycoconjugates can also be employed simultaneously to generate a mixed glycocalyx with tunable composition and organization.					
29927116	3	60	theme	glycan	620:625	arg1	interactions					627:638	glycan interactions	620:638	glycan interactions	620:638	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	1	61	theme	synthetic	124:132	arg1	glycoprotein					134:145	synthetic glycoprotein	124:145	synthetic glycoprotein	124:145	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	1	62	theme	cell	228:231	arg1	display					241:247	cell surface display	228:247	cell surface display of specific glycan structures in predefined nanoscale arrangements	228:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	3	63	theme	cell	676:679	arg1	differentiation					681:695	stem cell differentiation	671:695	stem cell differentiation	671:695	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	8	64	theme	glycoconjugates	1426:1440	arg1	Combinations					1410:1421	Combinations	1410:1421	Combinations of glycoconjugates	1410:1440	Combinations of glycoconjugates can also be employed simultaneously to generate a mixed glycocalyx with tunable composition and organization.					
29927116	3	65	theme	precise	508:514	arg1	study					516:520	the precise study	504:520	the precise study of individual glycans at different regions of the glycocalyx	504:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	7	66	theme	dose-dependent	1387:1400	arg1	manner					1402:1407	a dose-dependent manner	1385:1407	a dose-dependent manner	1385:1407	Flow cytometry data reveals that cell surface remodeling with either glycoconjugate mimetic occurs efficiently in a dose-dependent manner.					
29927116	1	67	theme	surface	233:239	arg1	display					241:247	cell surface display	228:247	cell surface display of specific glycan structures in predefined nanoscale arrangements	228:314	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	9	68	theme	&	1573:1573	arg1	Sons					1575:1578	John Wiley & Sons	1562:1578	John Wiley & Sons	1562:1578	© 2018 by John Wiley & Sons, Inc.					
29927116	5	69	theme	cell	1108:1111	arg1	membrane					1113:1120	the cell membrane	1104:1120	the cell membrane	1104:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	70	theme	anchor	1056:1061	arg1	glycans					1063:1069	anchor glycans	1056:1069	anchor glycans in dynamic 2D arrays proximal to the cell membrane	1056:1120	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	5	71	theme	3D	934:935	arg1	ligands					949:955	3D multivalent ligands	934:955	3D multivalent ligands projecting away from the cell surface	934:993	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
29927116	2	72	gly	heterogeneity	338:350	arg1	glycans					355:361	glycans	355:361	glycans found on the cell surface or the glycocalyx	355:405	The complex chemical heterogeneity of glycans found on the cell surface or the glycocalyx renders analysis of the individual contributions of glycans difficult.					
29927116	6	73	theme	α-2,6-linked	1244:1255	arg1	acids					1264:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	α-2,3- or α-2,6-linked sialic acids	1234:1268	Following washing, mimetic incorporation and glycan display can be analyzed using lectins with specificity for α-2,3- or α-2,6-linked sialic acids.					
29927116	1	74	theme	glycolipid	151:160	arg1	mimetics					162:169	glycolipid mimetics	151:169	glycolipid mimetics	151:169	This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale arrangements.					
29927116	3	75	from	regions	557:563	arg1	study					516:520	the precise study	504:520	the precise study of individual glycans at different regions of the glycocalyx	504:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	3	75	from	regions	557:563	arg1	glycans					536:542	individual glycans	525:542	individual glycans at different regions of the glycocalyx	525:581	This technique allows for the precise study of individual glycans at different regions of the glycocalyx, and may be useful for interrogating glycan interactions in infection or immunity or in stem cell differentiation.					
29927116	5	76	theme	α-2,3-sialyllactose	852:870	arg1	glycans					872:878	α-2,3-sialyllactose glycans	852:878	α-2,3-sialyllactose glycans	852:878	Synthetic glycopolymers bearing α-2,3-sialyllactose glycans are used to decorate cellular surfaces in the form of 3D multivalent ligands projecting away from the cell surface, while α-2,6-sialyllactose glycolipid conjugates are used to anchor glycans in dynamic 2D arrays proximal to the cell membrane.					
31485742	8	0	theme	indolicidin	1160:1170	arg1	analogue					1172:1179	an indolicidin analogue	1157:1179	an indolicidin analogue	1157:1179	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	1	1	theme	cathelicidin	146:157	arg1	family					159:164	cathelicidin family	146:164	cathelicidin family	146:164	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	8	2	theme	aromatic	1233:1240	arg1	acid					1248:1251	aromatic amino acid	1233:1251	aromatic amino acid	1233:1251	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	8	2	theme	aromatic	1233:1240	arg1	strategy					1297:1304	a promising strategy	1285:1304	a promising strategy for the first time	1285:1323	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	1	3	theme	family	159:164	arg1	Indolicidin					119:129	Indolicidin	119:129	Indolicidin	119:129	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	1	3	theme	family	159:164	arg1	member					136:141	a member	134:141	a member of cathelicidin family which displays broad spectrum antimicrobial activity	134:217	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	6	4	theme	peptide	916:922	arg1	killing					831:837	bacterial killing	821:837	bacterial killing	821:837	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	6	4	theme	peptide	916:922	arg1	stability					851:859	functional stability	840:859	functional stability	840:859	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	6	4	theme	peptide	916:922	arg1	binding					866:872	LPS binding	862:872	LPS binding	862:872	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	6	4	theme	peptide	916:922	arg1	potential					899:907	cytokine inhibitory potential	879:907	cytokine inhibitory potential of the peptide	879:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	6	5	theme	killing	831:837	arg1	mode					813:816	The mode	809:816	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide	809:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	4	6	theme	macrophage	676:685	arg1	cells					687:691	macrophage cells	676:691	macrophage cells	676:691	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	3	7	theme	glycosylation	383:395	arg1	strategy					397:404	glycosylation strategy	383:404	glycosylation strategy	383:404	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	4	8	theme	decreased	632:640	arg1	toxicity					642:649	decreased toxicity	632:649	decreased toxicity against erythrocytes and macrophage cells	632:691	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	3	9	from	effects	494:500	arg1	it					514:515	it	514:515	it	514:515	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	6	10	theme	bacterial	821:829	arg1	killing					831:837	bacterial killing	821:837	bacterial killing	821:837	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	4	11	theme	therapeutic	711:721	arg1	selectivity					723:733	a higher therapeutic selectivity	702:733	a higher therapeutic selectivity	702:733	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	12	theme	K7	603:604	arg1	peptide					585:591	a potent antibacterial glycosylated peptide	549:591	a potent antibacterial glycosylated peptide	549:591	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	12	theme	K7	603:604	arg1	indolicidin					606:616	K7]indolicidin	603:616	K7]indolicidin	603:616	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	8	13	theme	therapeutic	1195:1205	arg1	potential					1207:1215	improved therapeutic potential	1186:1215	improved therapeutic potential	1186:1215	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	3	14	theme	sugar	505:509	arg1	effects					494:500	structural and functional effects	468:500	structural and functional effects of sugar on it	468:515	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	2	15	theme	huge	298:301	arg1	limitation					303:312	a huge limitation	296:312	a huge limitation to its probable therapeutic application	296:352	Severe toxicity and aggregation propensity associated with indolicidin pose a huge limitation to its probable therapeutic application.					
31485742	7	16	from	structure	1006:1014	arg1	Absence					972:978	Absence	972:978	Absence of significant changes in structure upon glycosylation	972:1033	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	3	17	theme	structural	468:477	arg1	effects					494:500	structural and functional effects	468:500	structural and functional effects of sugar on it	468:515	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	0	18	theme	glycosylation	95:107	arg1	strategy					109:116	a glycosylation strategy	93:116	a glycosylation strategy	93:116	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	6	19	theme	potential	899:907	arg1	mode					813:816	The mode	809:816	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide	809:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	5	20	theme	peptide	800:806	arg1	solubility					782:791	the solubility	778:791	the solubility of the peptide	778:806	The incorporation of sugar also increased the solubility of the peptide.					
31485742	8	21	theme	amino	1271:1275	arg1	acid					1277:1280	glycosylated amino acid	1258:1280	glycosylated amino acid	1258:1280	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	6	22	theme	inhibitory	888:897	arg1	potential					899:907	cytokine inhibitory potential	879:907	cytokine inhibitory potential of the peptide	879:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	1	23	theme	broad	181:185	arg1	activity					210:217	broad spectrum antimicrobial activity	181:217	broad spectrum antimicrobial activity	181:217	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	4	24	theme	glycosylated	572:583	arg1	[βGlc-T9					594:601	[βGlc-T9	594:601	[βGlc-T9	594:601	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	24	theme	glycosylated	572:583	arg1	peptide					585:591	a potent antibacterial glycosylated peptide	549:591	a potent antibacterial glycosylated peptide	549:591	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	24	theme	glycosylated	572:583	arg1	indolicidin					606:616	K7]indolicidin	603:616	K7]indolicidin	603:616	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	7	25	theme	peptide	1106:1112	arg1	action					1092:1097	action	1092:1097	action of the peptide	1092:1112	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	2	26	theme	therapeutic	330:340	arg1	application					342:352	its probable therapeutic application	317:352	its probable therapeutic application	317:352	Severe toxicity and aggregation propensity associated with indolicidin pose a huge limitation to its probable therapeutic application.					
31485742	8	27	theme	promising	1287:1295	arg1	strategy					1297:1304	a promising strategy	1285:1304	a promising strategy for the first time	1285:1323	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	8	27	theme	promising	1287:1295	arg1	acid					1248:1251	aromatic amino acid	1233:1251	aromatic amino acid	1233:1251	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	1	28	theme	spectrum	187:194	arg1	activity					210:217	broad spectrum antimicrobial activity	181:217	broad spectrum antimicrobial activity	181:217	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	3	29	theme	strategy	397:404	arg1	use					376:378	the use	372:378	the use of glycosylation strategy to design an analogue of indolicidin	372:441	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	4	30	theme	antibacterial	558:570	arg1	[βGlc-T9					594:601	[βGlc-T9	594:601	[βGlc-T9	594:601	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	30	theme	antibacterial	558:570	arg1	peptide					585:591	a potent antibacterial glycosylated peptide	549:591	a potent antibacterial glycosylated peptide	549:591	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	30	theme	antibacterial	558:570	arg1	indolicidin					606:616	K7]indolicidin	603:616	K7]indolicidin	603:616	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	7	31	theme	action	1092:1097	arg1	functions					1070:1078	the possibly retained functions	1048:1078	the possibly retained functions	1048:1078	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	7	31	theme	action	1092:1097	arg1	mode					1084:1087	mode	1084:1087	mode of action of the peptide	1084:1112	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	8	32	gly	glycosylated	1258:1269	arg1	acid					1277:1280	glycosylated amino acid	1258:1280	glycosylated amino acid	1258:1280	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	1	33	theme	antimicrobial	196:208	arg1	activity					210:217	broad spectrum antimicrobial activity	181:217	broad spectrum antimicrobial activity	181:217	Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity.					
31485742	6	34	theme	LPS	862:864	arg1	binding					866:872	LPS binding	862:872	LPS binding	862:872	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	6	35	theme	cytokine	879:886	arg1	potential					899:907	cytokine inhibitory potential	879:907	cytokine inhibitory potential of the peptide	879:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	4	36	theme	higher	704:709	arg1	selectivity					723:733	a higher therapeutic selectivity	702:733	a higher therapeutic selectivity	702:733	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	2	37	dep	toxicity	227:234	arg1	propensity					252:261	propensity	252:261	propensity	252:261	Severe toxicity and aggregation propensity associated with indolicidin pose a huge limitation to its probable therapeutic application.					
31485742	3	38	theme	indolicidin	431:441	arg1	analogue					419:426	an analogue	416:426	an analogue of indolicidin	416:441	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	4	39	theme	peptide	585:591	arg1	design					539:544	the design	535:544	the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells	535:691	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	3	40	theme	functional	483:492	arg1	effects					494:500	structural and functional effects	468:500	structural and functional effects of sugar on it	468:515	We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it.					
31485742	0	41	theme	analogue	35:42	arg1	Design					0:5	Design	0:5	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin,	0:85	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	8	42	theme	amino	1242:1246	arg1	acid					1248:1251	aromatic amino acid	1233:1251	aromatic amino acid	1233:1251	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	8	42	theme	amino	1242:1246	arg1	strategy					1297:1304	a promising strategy	1285:1304	a promising strategy for the first time	1285:1323	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	0	43	theme	improved	26:33	arg1	indolicidin					74:84	indolicidin	74:84	indolicidin	74:84	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	0	43	theme	improved	26:33	arg1	analogue					35:42	therapeutically improved analogue	10:42	therapeutically improved analogue of the antimicrobial peptide	10:71	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	8	44	theme	improved	1186:1193	arg1	potential					1207:1215	improved therapeutic potential	1186:1215	improved therapeutic potential	1186:1215	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	0	45	theme	antimicrobial	51:63	arg1	peptide					65:71	the antimicrobial peptide	47:71	the antimicrobial peptide	47:71	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	6	46	theme	binding	866:872	arg1	mode					813:816	The mode	809:816	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide	809:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	4	47	gly	glycosylated	572:583	arg1	[βGlc-T9					594:601	[βGlc-T9	594:601	[βGlc-T9	594:601	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	47	gly	glycosylated	572:583	arg1	peptide					585:591	a potent antibacterial glycosylated peptide	549:591	a potent antibacterial glycosylated peptide	549:591	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	47	gly	glycosylated	572:583	arg1	indolicidin					606:616	K7]indolicidin	603:616	K7]indolicidin	603:616	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	48	theme	potent	551:556	arg1	[βGlc-T9					594:601	[βGlc-T9	594:601	[βGlc-T9	594:601	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	48	theme	potent	551:556	arg1	peptide					585:591	a potent antibacterial glycosylated peptide	549:591	a potent antibacterial glycosylated peptide	549:591	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	4	48	theme	potent	551:556	arg1	indolicidin					606:616	K7]indolicidin	603:616	K7]indolicidin	603:616	Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity.					
31485742	2	49	theme	Severe	220:225	arg1	toxicity					227:234	Severe toxicity	220:234	Severe toxicity	220:234	Severe toxicity and aggregation propensity associated with indolicidin pose a huge limitation to its probable therapeutic application.					
31485742	8	50	theme	first	1314:1318	arg1	time					1320:1323	the first time	1310:1323	the first time	1310:1323	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	7	51	theme	significant	983:993	arg1	changes					995:1001	significant changes	983:1001	significant changes in structure upon glycosylation	983:1033	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	7	52	theme	retained	1061:1068	arg1	functions					1070:1078	the possibly retained functions	1048:1078	the possibly retained functions	1048:1078	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	6	53	theme	stability	851:859	arg1	mode					813:816	The mode	809:816	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide	809:922	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	8	54	theme	glycosylated	1258:1269	arg1	acid					1277:1280	glycosylated amino acid	1258:1280	glycosylated amino acid	1258:1280	Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time.					
31485742	6	55	theme	functional	840:849	arg1	stability					851:859	functional stability	840:859	functional stability	840:859	The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation.					
31485742	7	56	from	changes	995:1001	arg1	structure					1006:1014	structure	1006:1014	structure	1006:1014	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	0	57	theme	peptide	65:71	arg1	indolicidin					74:84	indolicidin	74:84	indolicidin	74:84	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	0	57	theme	peptide	65:71	arg1	analogue					35:42	therapeutically improved analogue	10:42	therapeutically improved analogue of the antimicrobial peptide	10:71	Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy.					
31485742	7	58	theme	changes	995:1001	arg1	Absence					972:978	Absence	972:978	Absence of significant changes in structure upon glycosylation	972:1033	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	7	59	from	Absence	972:978	arg1	structure					1006:1014	structure	1006:1014	structure	1006:1014	Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide.					
31485742	5	60	theme	sugar	757:761	arg1	incorporation					740:752	The incorporation	736:752	The incorporation of sugar	736:761	The incorporation of sugar also increased the solubility of the peptide.					
31400301	4	0	theme	first	454:458	arg1	study					460:464	the first study	450:464	the first study to profile glycans in PTC tissues	450:498	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	4	0	theme	first	454:458	arg1	this					442:445	this	442:445	this	442:445	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	2	1	from	regulation	274:283	arg1	glands					325:330	thyroid glands	317:330	thyroid glands	317:330	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	4	2	theme	profile	469:475	arg1	glycans					477:483	profile glycans	469:483	profile glycans	469:483	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	5	3	theme	tissues	604:610	arg1	N-glycans					551:559	the N-glycans	547:559	the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study	547:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	1	4	theme	incidence	143:151	arg1	rate					153:156	rate	153:156	rate	153:156	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	7	5	theme	N-glycan	840:847	arg1	p < 0.001					863:871	p < 0.001	863:871	p < 0.001	863:871	The statistical analyses showed significant differences including six N-glycan compositions (p < 0.001) between patients and controls.					
31400301	7	5	theme	N-glycan	840:847	arg1	compositions					849:860	six N-glycan compositions	836:860	six N-glycan compositions (p < 0.001)	836:872	The statistical analyses showed significant differences including six N-glycan compositions (p < 0.001) between patients and controls.					
31400301	9	6	theme	N-glycan	1202:1209	arg1	traits					1211:1216	six different N-glycan traits	1188:1216	six different N-glycan traits	1188:1216	Besides, a significant difference was found in six different N-glycan traits.					
31400301	4	7	from	study	460:464	arg1	tissues					492:498	PTC tissues	488:498	PTC tissues	488:498	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	8	8	theme	them	936:939	arg1	them					936:939	them	936:939	them	936:939	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	8	theme	them	936:939	arg1	four					928:931	four	928:931	four	928:931	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	9	link	α2,6-linked	988:998	arg1	acid					1007:1010	α2,6-linked sialic acid	988:1010	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	10	dep	E	985:985	arg1	L					1013:1013	L	1013:1013	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	10	dep	E	985:985	arg1	acid					1007:1010	α2,6-linked sialic acid	988:1010	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	10	dep	E	985:985	arg1	acid					1035:1038	α2,3-linked sialic acid	1016:1038	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	5	11	theme	patients	615:622	arg1	tissues					604:610	formalin-fixed paraffin-embedded (FFPE) tissues	564:610	formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study	564:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	0	12	theme	N-glycan	0:7	arg1	profiling					9:17	N-glycan profiling	0:17	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS	0:72	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS.					
31400301	5	13	theme	formalin-fixed	564:577	arg1	tissues					604:610	formalin-fixed paraffin-embedded (FFPE) tissues	564:610	formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study	564:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	1	14	theme	Papillary	75:83	arg1	type					114:117	a type	112:117	a type of thyroid cancer whose incidence rate has increased recently all over the world	112:198	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	1	14	theme	Papillary	75:83	arg1	PTC					104:106	PTC	104:106	PTC	104:106	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	1	14	theme	Papillary	75:83	arg1	carcinoma					93:101	Papillary thyroid carcinoma	75:101	Papillary thyroid carcinoma (PTC)	75:107	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	8	15	link	α2,3-linked	1016:1026	arg1	acid					1035:1038	α2,3-linked sialic acid	1016:1038	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	5	16	dep	paraffin-embedded	579:595	arg1	FFPE					598:601	FFPE	598:601	FFPE	598:601	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	8	17	theme	α2,6-linked	988:998	arg1	acid					1007:1010	α2,6-linked sialic acid	988:1010	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	6	18	theme	N-glycan	728:735	arg1	compositions					737:748	35 enzymatically released N-glycan compositions	702:748	35 enzymatically released N-glycan compositions	702:748	Using MALDI-TOF(/TOF)-MS, 35 enzymatically released N-glycan compositions were characterized.					
31400301	1	19	theme	thyroid	85:91	arg1	type					114:117	a type	112:117	a type of thyroid cancer whose incidence rate has increased recently all over the world	112:198	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	1	19	theme	thyroid	85:91	arg1	PTC					104:106	PTC	104:106	PTC	104:106	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	1	19	theme	thyroid	85:91	arg1	carcinoma					93:101	Papillary thyroid carcinoma	75:101	Papillary thyroid carcinoma (PTC)	75:107	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	7	20	theme	significant	802:812	arg1	differences					814:824	significant differences	802:824	significant differences including six N-glycan compositions (p < 0.001) between patients and controls	802:902	The statistical analyses showed significant differences including six N-glycan compositions (p < 0.001) between patients and controls.					
31400301	7	20	theme	significant	802:812	arg1	compositions					849:860	six N-glycan compositions	836:860	six N-glycan compositions (p < 0.001)	836:872	The statistical analyses showed significant differences including six N-glycan compositions (p < 0.001) between patients and controls.					
31400301	6	21	theme	released	719:726	arg1	compositions					737:748	35 enzymatically released N-glycan compositions	702:748	35 enzymatically released N-glycan compositions	702:748	Using MALDI-TOF(/TOF)-MS, 35 enzymatically released N-glycan compositions were characterized.					
31400301	2	22	theme	hormone	296:302	arg1	synthesis					304:312	thyroid hormone synthesis	288:312	thyroid hormone synthesis	288:312	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	3	23	theme	N-glycosylation	370:384	arg1	change					386:391	the N-glycosylation change	366:391	the N-glycosylation change in PTC	366:398	However, our knowledge regarding the N-glycosylation change in PTC is limited.					
31400301	4	24	theme	mass	503:506	arg1	spectrometry					508:519	mass spectrometry	503:519	mass spectrometry	503:519	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	2	25	theme	thyroid	288:294	arg1	synthesis					304:312	thyroid hormone synthesis	288:312	thyroid hormone synthesis	288:312	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	10	26	dep	PTC	1231:1233	arg1	follicular					1236:1245	follicular	1236:1245	follicular	1236:1245	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	10	26	dep	PTC	1231:1233	arg1	classical					1248:1256	classical	1248:1256	classical	1248:1256	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	10	26	dep	PTC	1231:1233	arg1	cell					1266:1269	hurtle cell	1259:1269	hurtle cell	1259:1269	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	8	27	dep	N-glycans	1087:1095	arg1	H9N2					1107:1110	H9N2	1107:1110	H9N2	1107:1110	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	27	dep	N-glycans	1087:1095	arg1	H8N2					1098:1101	H8N2	1098:1101	H8N2	1098:1101	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	27	dep	N-glycans	1087:1095	arg1	N-glycans					1087:1095	two N-glycans	1083:1095	two N-glycans (H8N2 and H9N2)	1083:1111	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	0	28	theme	papillary	22:30	arg1	tissues					50:56	papillary thyroid carcinoma tissues	22:56	papillary thyroid carcinoma tissues	22:56	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS.					
31400301	8	29	theme	sialic	1000:1005	arg1	acid					1007:1010	α2,6-linked sialic acid	988:1010	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	30	dep	H5N4E1	942:947	arg1	E					985:985	E	985:985	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	10	31	theme	hurtle	1259:1264	arg1	follicular					1236:1245	follicular	1236:1245	follicular	1236:1245	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	10	31	theme	hurtle	1259:1264	arg1	cell					1266:1269	hurtle cell	1259:1269	hurtle cell	1259:1269	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	5	32	theme	matched	648:654	arg1	study					669:673	a matched case-control study	646:673	a matched case-control study	646:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	10	33	theme	specific	1300:1307	arg1	change					1318:1323	specific N-glycan change	1300:1323	specific N-glycan change for each variant	1300:1340	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	0	34	theme	carcinoma	40:48	arg1	tissues					50:56	papillary thyroid carcinoma tissues	22:56	papillary thyroid carcinoma tissues	22:56	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS.					
31400301	5	35	theme	case-control	656:667	arg1	study					669:673	a matched case-control study	646:673	a matched case-control study	646:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	9	36	located	found	1179:1183	arg2	difference					1164:1173	a significant difference	1150:1173	a significant difference	1150:1173	Besides, a significant difference was found in six different N-glycan traits.					
31400301	9	36	located	found	1179:1183	arg1	traits					1211:1216	six different N-glycan traits	1188:1216	six different N-glycan traits	1188:1216	Besides, a significant difference was found in six different N-glycan traits.					
31400301	7	37	theme	statistical	774:784	arg1	analyses					786:793	The statistical analyses	770:793	The statistical analyses	770:793	The statistical analyses showed significant differences including six N-glycan compositions (p < 0.001) between patients and controls.					
31400301	0	38	theme	thyroid	32:38	arg1	tissues					50:56	papillary thyroid carcinoma tissues	22:56	papillary thyroid carcinoma tissues	22:56	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS.					
31400301	8	39	theme	α2,3-linked	1016:1026	arg1	acid					1035:1038	α2,3-linked sialic acid	1016:1038	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	5	40	dep	formalin-fixed	564:577	arg1	paraffin-embedded					579:595	paraffin-embedded	579:595	paraffin-embedded	579:595	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	0	41	theme	tissues	50:56	arg1	profiling					9:17	N-glycan profiling	0:17	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS	0:72	N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS.					
31400301	9	42	theme	significant	1152:1162	arg1	difference					1164:1173	a significant difference	1150:1173	a significant difference	1150:1173	Besides, a significant difference was found in six different N-glycan traits.					
31400301	2	43	theme	thyroid	317:323	arg1	glands					325:330	thyroid glands	317:330	thyroid glands	317:330	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	4	44	theme	PTC	488:490	arg1	tissues					492:498	PTC tissues	488:498	PTC tissues	488:498	To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry.					
31400301	8	45	theme	sialic	1028:1033	arg1	acid					1035:1038	α2,3-linked sialic acid	1016:1038	E: α2,6-linked sialic acid; L: α2,3-linked sialic acid	985:1038	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	10	46	theme	N-glycan	1309:1316	arg1	change					1318:1323	specific N-glycan change	1300:1323	specific N-glycan change for each variant	1300:1340	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	10	47	theme	PTC	1231:1233	arg1	Variants					1219:1226	Variants	1219:1226	Variants of PTC (follicular, classical, hurtle cell)	1219:1270	Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant.					
31400301	8	48	dep	four	928:931	arg1	H5N4E1					942:947	H5N4E1	942:947	H5N4E1	942:947	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	48	dep	four	928:931	arg1	H5N4F1E2					975:982	H5N4F1E2	975:982	H5N4F1E2	975:982	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	48	dep	four	928:931	arg1	H5N4F1L1E1					960:969	H5N4F1L1E1	960:969	H5N4F1L1E1	960:969	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	8	48	dep	four	928:931	arg1	H5N4F1E1					950:957	H5N4F1E1	950:957	H5N4F1E1	950:957	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	5	49	from	PTC	639:641	arg1	study					669:673	a matched case-control study	646:673	a matched case-control study	646:673	Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study.					
31400301	2	50	theme	synthesis	304:312	arg1	regulation					274:283	the regulation	270:283	the regulation of thyroid hormone synthesis in thyroid glands	270:330	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	9	51	theme	different	1192:1200	arg1	traits					1211:1216	six different N-glycan traits	1188:1216	six different N-glycan traits	1188:1216	Besides, a significant difference was found in six different N-glycan traits.					
31400301	8	52	theme	PTC	1062:1064	arg1	tissues					1066:1072	PTC tissues	1062:1072	PTC tissues	1062:1072	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	2	53	theme	post-translational	228:245	arg1	PTM					261:263	PTM	261:263	PTM	261:263	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	2	53	theme	post-translational	228:245	arg1	modification					247:258	a crucial post-translational modification	218:258	a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands	218:330	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	2	53	theme	post-translational	228:245	arg1	Glycosylation					201:213	Glycosylation	201:213	Glycosylation	201:213	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	6	54	theme	MALDI-TOF	682:690	arg1	-MS					697:699	MALDI-TOF(/TOF)-MS	682:699	MALDI-TOF(/TOF)-MS	682:699	Using MALDI-TOF(/TOF)-MS, 35 enzymatically released N-glycan compositions were characterized.					
31400301	1	55	theme	thyroid	122:128	arg1	cancer					130:135	thyroid cancer	122:135	thyroid cancer	122:135	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	8	56	dep	determined	912:921	arg1	whereas					1075:1081	whereas	1075:1081	whereas	1075:1081	It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated.					
31400301	3	57	from	change	386:391	arg1	PTC					396:398	PTC	396:398	PTC	396:398	However, our knowledge regarding the N-glycosylation change in PTC is limited.					
31400301	2	58	theme	crucial	220:226	arg1	PTM					261:263	PTM	261:263	PTM	261:263	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	2	58	theme	crucial	220:226	arg1	modification					247:258	a crucial post-translational modification	218:258	a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands	218:330	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	2	58	theme	crucial	220:226	arg1	Glycosylation					201:213	Glycosylation	201:213	Glycosylation	201:213	Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands.					
31400301	1	59	theme	cancer	130:135	arg1	type					114:117	a type	112:117	a type of thyroid cancer whose incidence rate has increased recently all over the world	112:198	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
31400301	1	59	theme	cancer	130:135	arg1	carcinoma					93:101	Papillary thyroid carcinoma	75:101	Papillary thyroid carcinoma (PTC)	75:107	Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world.					
29502191	12	0	theme	carbohydrate-binding	1800:1819	arg1	proteins					1821:1828	carbohydrate-binding proteins	1800:1828	carbohydrate-binding proteins	1800:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	9	1	theme	unusual	1169:1175	arg1	N-glycans					1185:1193	these unusual complex N-glycans	1163:1193	these unusual complex N-glycans	1163:1193	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	12	2	theme	cell	1759:1762	arg1	biology					1764:1770	cancer cell biology	1752:1770	cancer cell biology	1752:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	10	3	theme	cancer	1353:1358	arg1	cells					1360:1364	cancer cells	1353:1364	cancer cells	1353:1364	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	9	4	theme	N-glycans	1185:1193	arg1	presence					1151:1158	The presence	1147:1158	The presence of these unusual complex N-glycans	1147:1193	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	9	5	gly	glycoproteins	1241:1253	arg1	glycoproteins					1241:1253	cellular glycoproteins	1232:1253	cellular glycoproteins	1232:1253	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	2	6	theme	structures	298:307	arg1	formation					261:269	the formation	257:269	the formation of highly branched complex structures within the Golgi apparatus	257:334	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	12	7	theme	"	1730:1730	arg1	oligosaccharides					1732:1747	"bisected" oligosaccharides	1721:1747	"bisected" oligosaccharides in cancer cell biology	1721:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	5	8	theme	cellular	618:625	arg1	context					627:633	the cellular context	614:633	the cellular context	614:633	However, the effects of GnT-III may be more diverse and depend on the cellular context.					
29502191	11	9	theme	"	1548:1548	arg1	GlcNAc					1550:1555	"bisecting" GlcNAc	1538:1555	"bisecting" GlcNAc	1538:1555	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	7	10	theme	labeled	839:845	arg1	N-glycans					847:855	2-AA labeled N-glycans	834:855	2-AA labeled N-glycans	834:855	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	8	11	theme	range	1017:1021	arg1	modification					993:1004	the modification	989:1004	the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them	989:1144	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	6	12	from	analysis	670:677	arg1	cells					758:762	WM266-4-GnT-III metastatic melanoma cells	722:762	WM266-4-GnT-III metastatic melanoma cells	722:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	9	13	theme	cellular	1232:1239	arg1	glycoproteins					1241:1253	cellular glycoproteins	1232:1253	cellular glycoproteins	1232:1253	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	8	14	with	introduction	1048:1059	arg1	structures					1124:1133	highly branched complex structures	1100:1133	highly branched complex structures among them	1100:1144	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	12	15	with	interactions	1782:1793	arg1	proteins					1821:1828	carbohydrate-binding proteins	1800:1828	carbohydrate-binding proteins	1800:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	11	16	theme	bisecting	1539:1547	arg1	GlcNAc					1550:1555	"bisecting" GlcNAc	1538:1555	"bisecting" GlcNAc	1538:1555	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	2	17	theme	branched	281:288	arg1	structures					298:307	highly branched complex structures	274:307	highly branched complex structures	274:307	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	12	18	theme	bisected	1722:1729	arg1	oligosaccharides					1732:1747	"bisected" oligosaccharides	1721:1747	"bisected" oligosaccharides in cancer cell biology	1721:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	0	19	theme	metastatic	77:86	arg1	cells					97:101	metastatic melanoma cells	77:101	metastatic melanoma cells	77:101	The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells.					
29502191	4	20	theme	cancer	508:513	arg1	cells					515:519	cancer cells	508:519	cancer cells	508:519	Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo.					
29502191	10	21	theme	branched	1441:1448	arg1	glycans					1450:1456	highly branched glycans	1434:1456	highly branched glycans	1434:1456	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	8	22	theme	N-glycan	1026:1033	arg1	types					1035:1039	N-glycan types	1026:1039	N-glycan types	1026:1039	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	12	23	theme	biological	1698:1707	arg1	function					1709:1716	the biological function	1694:1716	the biological function of "bisected" oligosaccharides in cancer cell biology	1694:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	3	24	theme	other	377:381	arg1	GnT-V					422:426	GnT-V	422:426	GnT-V	422:426	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	3	24	theme	other	377:381	arg1	transferases					390:401	other GlcNAc transferases	377:401	other GlcNAc transferases such as GnT-IV and GnT-V	377:426	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	3	24	theme	other	377:381	arg1	GnT-IV					411:416	GnT-IV	411:416	GnT-IV	411:416	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	11	25	theme	N-glycans	1505:1513	arg1	modification					1476:1487	the modification	1472:1487	the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc	1472:1555	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	0	26	from	overexpression	59:72	arg1	cells					97:101	metastatic melanoma cells	77:101	metastatic melanoma cells	77:101	The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells.					
29502191	6	27	theme	effect	686:691	arg1	analysis					670:677	the detailed glycomic analysis	648:677	the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells	648:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	12	28	with	function	1709:1716	arg1	proteins					1821:1828	carbohydrate-binding proteins	1800:1828	carbohydrate-binding proteins	1800:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	12	29	theme	further	1675:1681	arg1	studies					1683:1689	further studies	1675:1689	further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins	1675:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	8	30	from	overexpression	942:955	arg1	melanoma					971:978	melanoma	971:978	melanoma	971:978	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	4	31	theme	potential	495:503	arg1	inhibitor					467:475	an inhibitor	464:475	an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo	464:545	Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo.					
29502191	4	31	theme	potential	495:503	arg1	GnT-III					439:445	GnT-III	439:445	GnT-III	439:445	Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo.					
29502191	9	32	with	interactions	1216:1227	arg1	PHA-L					1264:1268	the PHA-L	1260:1268	the PHA-L	1260:1268	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	5	33	theme	GnT-III	572:578	arg1	effects					561:567	the effects	557:567	the effects of GnT-III	557:578	However, the effects of GnT-III may be more diverse and depend on the cellular context.					
29502191	5	33	theme	GnT-III	572:578	arg1	diverse					592:598	diverse	592:598	diverse	592:598	However, the effects of GnT-III may be more diverse and depend on the cellular context.					
29502191	3	34	theme	transferases	390:401	arg1	action					367:372	the action	363:372	the action of other GlcNAc transferases such as GnT-IV and GnT-V	363:426	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	6	35	theme	glycomic	661:668	arg1	analysis					670:677	the detailed glycomic analysis	648:677	the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells	648:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	8	36	theme	bisecting	1069:1077	arg1	residue					1087:1093	the "bisecting" GlcNAc residue	1064:1093	the "bisecting" GlcNAc residue	1064:1093	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	1	37	theme	branched	128:135	arg1	N-glycans					137:145	highly branched N-glycans	121:145	highly branched N-glycans	121:145	GnT-III modifies highly branched N-glycans.					
29502191	0	38	theme	N-acetylglucosaminyltransferase	23:53	arg1	effect					13:18	The glycomic effect	0:18	The glycomic effect of N-acetylglucosaminyltransferase III	0:57	The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells.					
29502191	8	39	theme	GlcNAc	1080:1085	arg1	residue					1087:1093	the "bisecting" GlcNAc residue	1064:1093	the "bisecting" GlcNAc residue	1064:1093	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	10	40	theme	GnT-III	1342:1348	arg1	activity					1330:1337	elevated activity	1321:1337	elevated activity of GnT-III in cancer cells	1321:1364	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	12	41	theme	oligosaccharides	1732:1747	arg1	interactions					1782:1793	their interactions	1776:1793	their interactions with carbohydrate-binding proteins	1776:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	12	41	theme	oligosaccharides	1732:1747	arg1	function					1709:1716	the biological function	1694:1716	the biological function of "bisected" oligosaccharides in cancer cell biology	1694:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	6	42	theme	metastatic	738:747	arg1	cells					758:762	WM266-4-GnT-III metastatic melanoma cells	722:762	WM266-4-GnT-III metastatic melanoma cells	722:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	8	43	theme	branched	1107:1114	arg1	structures					1124:1133	highly branched complex structures	1100:1133	highly branched complex structures among them	1100:1144	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	6	44	from	cells	758:762	arg1	analysis					670:677	the detailed glycomic analysis	648:677	the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells	648:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	6	45	theme	GnT-III	696:702	arg1	overexpression					704:717	GnT-III overexpression	696:717	GnT-III overexpression	696:717	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	7	46	theme	secreted	905:912	arg1	proteins					914:921	membrane-attached and secreted proteins	883:921	membrane-attached and secreted proteins	883:921	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	12	47	from	interactions	1782:1793	arg1	biology					1764:1770	cancer cell biology	1752:1770	cancer cell biology	1752:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	11	48	theme	plant	1640:1644	arg1	lectins					1646:1652	plant lectins	1640:1652	plant lectins	1640:1652	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	9	49	theme	complex	1177:1183	arg1	N-glycans					1185:1193	these unusual complex N-glycans	1163:1193	these unusual complex N-glycans	1163:1193	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	2	50	theme	Golgi	320:324	arg1	apparatus					326:334	the Golgi apparatus	316:334	the Golgi apparatus	316:334	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	7	51	theme	membrane-attached	883:899	arg1	proteins					914:921	membrane-attached and secreted proteins	883:921	membrane-attached and secreted proteins	883:921	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	11	52	theme	carbohydrate-binding	1602:1621	arg1	lectins					1646:1652	plant lectins	1640:1652	plant lectins	1640:1652	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	11	52	theme	carbohydrate-binding	1602:1621	arg1	proteins					1623:1630	carbohydrate-binding proteins	1602:1630	carbohydrate-binding proteins such as plant lectins	1602:1652	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	2	53	dep	catalyze	206:213	arg1	modulate					248:255	modulate	248:255	modulate the formation of highly branched complex structures within the Golgi apparatus	248:334	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	8	54	theme	broad	1011:1015	arg1	range					1017:1021	a broad range	1009:1021	a broad range of N-glycan types	1009:1039	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	6	55	from	effect	686:691	arg1	cells					758:762	WM266-4-GnT-III metastatic melanoma cells	722:762	WM266-4-GnT-III metastatic melanoma cells	722:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	9	56	theme	stronger	1207:1214	arg1	interactions					1216:1227	stronger interactions	1207:1227	stronger interactions of cellular glycoproteins with the PHA-L	1207:1268	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	12	57	theme	cancer	1752:1757	arg1	biology					1764:1770	cancer cell biology	1752:1770	cancer cell biology	1752:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	10	58	theme	total	1397:1401	arg1	abrogation					1403:1412	a total abrogation	1395:1412	a total abrogation of the formation of highly branched glycans	1395:1456	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	7	59	theme	N-glycans	847:855	arg1	HILIC-HPLC					820:829	HILIC-HPLC	820:829	HILIC-HPLC of 2-AA labeled N-glycans	820:855	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	9	60	theme	glycoproteins	1241:1253	arg1	interactions					1216:1227	stronger interactions	1207:1227	stronger interactions of cellular glycoproteins with the PHA-L	1207:1268	The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L.					
29502191	7	61	theme	2-AA	834:837	arg1	N-glycans					847:855	2-AA labeled N-glycans	834:855	2-AA labeled N-glycans	834:855	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	12	62	from	function	1709:1716	arg1	biology					1764:1770	cancer cell biology	1752:1770	cancer cell biology	1752:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	11	63	theme	GlcNAc	1550:1555	arg1	introduction					1522:1533	the introduction	1518:1533	the introduction of "bisecting" GlcNAc	1518:1555	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	2	64	theme	complex	290:296	arg1	structures					298:307	highly branched complex structures	274:307	highly branched complex structures	274:307	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	0	65	theme	melanoma	88:95	arg1	cells					97:101	metastatic melanoma cells	77:101	metastatic melanoma cells	77:101	The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells.					
29502191	4	66	theme	cells	515:519	arg1	potential					495:503	the metastatic potential	480:503	the metastatic potential of cancer cells	480:519	Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo.					
29502191	12	67	from	studies	1683:1689	arg1	interactions					1782:1793	their interactions	1776:1793	their interactions with carbohydrate-binding proteins	1776:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	12	67	from	studies	1683:1689	arg1	function					1709:1716	the biological function	1694:1716	the biological function of "bisected" oligosaccharides in cancer cell biology	1694:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	8	68	theme	types	1035:1039	arg1	range					1017:1021	a broad range	1009:1021	a broad range of N-glycan types	1009:1039	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	11	69	theme	pre-existing	1492:1503	arg1	N-glycans					1505:1513	pre-existing N-glycans	1492:1513	pre-existing N-glycans	1492:1513	In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins.					
29502191	3	70	theme	GlcNAc	383:388	arg1	GnT-V					422:426	GnT-V	422:426	GnT-V	422:426	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	3	70	theme	GlcNAc	383:388	arg1	transferases					390:401	other GlcNAc transferases	377:401	other GlcNAc transferases such as GnT-IV and GnT-V	377:426	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	3	70	theme	GlcNAc	383:388	arg1	GnT-IV					411:416	GnT-IV	411:416	GnT-IV	411:416	While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V.					
29502191	4	71	theme	metastatic	484:493	arg1	potential					495:503	the metastatic potential	480:503	the metastatic potential of cancer cells	480:519	Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo.					
29502191	10	72	theme	formation	1421:1429	arg1	abrogation					1403:1412	a total abrogation	1395:1412	a total abrogation of the formation of highly branched glycans	1395:1456	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	0	73	theme	glycomic	4:11	arg1	effect					13:18	The glycomic effect	0:18	The glycomic effect of N-acetylglucosaminyltransferase III	0:57	The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells.					
29502191	12	74	from	biology	1764:1770	arg1	interactions					1782:1793	their interactions	1776:1793	their interactions with carbohydrate-binding proteins	1776:1828	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	12	74	from	biology	1764:1770	arg1	function					1709:1716	the biological function	1694:1716	the biological function of "bisected" oligosaccharides in cancer cell biology	1694:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	10	75	theme	glycans	1450:1456	arg1	formation					1421:1429	the formation	1417:1429	the formation of highly branched glycans	1417:1456	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	10	76	theme	elevated	1321:1328	arg1	activity					1330:1337	elevated activity	1321:1337	elevated activity of GnT-III in cancer cells	1321:1364	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	8	77	theme	"	1078:1078	arg1	residue					1087:1093	the "bisecting" GlcNAc residue	1064:1093	the "bisecting" GlcNAc residue	1064:1093	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	2	78	theme	N-glycan	215:222	arg1	"					234:234	N-glycan "bisection"	215:234	N-glycan "bisection"	215:234	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	6	79	theme	detailed	652:659	arg1	analysis					670:677	the detailed glycomic analysis	648:677	the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells	648:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	8	80	theme	residue	1087:1093	arg1	introduction					1048:1059	the introduction	1044:1059	the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them	1044:1144	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	12	81	from	oligosaccharides	1732:1747	arg1	biology					1764:1770	cancer cell biology	1752:1770	cancer cell biology	1752:1770	Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.					
29502191	7	82	used	used	768:771	arg2	We					765:766	We	765:766	We	765:766	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	6	83	theme	melanoma	749:756	arg1	cells					758:762	WM266-4-GnT-III metastatic melanoma cells	722:762	WM266-4-GnT-III metastatic melanoma cells	722:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	8	84	theme	complex	1116:1122	arg1	structures					1124:1133	highly branched complex structures	1100:1133	highly branched complex structures among them	1100:1144	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	2	85	theme	bisection	225:233	arg1	"					234:234	N-glycan "bisection"	215:234	N-glycan "bisection"	215:234	N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus.					
29502191	6	86	theme	WM266-4-GnT-III	722:736	arg1	cells					758:762	WM266-4-GnT-III metastatic melanoma cells	722:762	WM266-4-GnT-III metastatic melanoma cells	722:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
29502191	7	87	theme	proteins	914:921	arg1	N-glycome					870:878	the N-glycome	866:878	the N-glycome of membrane-attached and secreted proteins	866:921	We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins.					
29502191	8	88	theme	GnT-III	960:966	arg1	overexpression					942:955	the overexpression	938:955	the overexpression of GnT-III in melanoma	938:978	We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them.					
29502191	10	89	from	activity	1330:1337	arg1	cells					1360:1364	cancer cells	1353:1364	cancer cells	1353:1364	Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans.					
29502191	6	90	theme	overexpression	704:717	arg1	effect					686:691	the effect	682:691	the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells	682:762	We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells.					
31669471	0	0	theme	growth	41:46	arg1	factor					48:53	insulin-like growth factor	28:53	insulin-like growth factor receptors	28:63	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	4	1	gly	glycosylation	687:699	arg1	receptors					712:720	receptors	712:720	receptors	712:720	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	2	2	theme	growth	385:390	arg1	factor					392:397	insulin-like growth factor	372:397	the insulin-like growth factor (IGF) receptors	368:413	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	2	2	theme	growth	385:390	arg1	IGF					400:402	IGF	400:402	IGF	400:402	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	5	3	attach	isolated	857:864	arg1	tissue					899:904	non-tumour colon tissue	882:904	non-tumour colon tissue	882:904	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	5	3	attach	isolated	857:864	arg2	proteins					848:855	the solubilised membrane proteins	823:855	the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer	823:939	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	5	3	attach	isolated	857:864	arg1	tumour					871:876	tumour	871:876	tumour	871:876	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	8	4	theme	Increased	1284:1292	arg1	levels					1294:1299	Increased levels	1284:1299	Increased levels of fucosylation and branched mannose structures	1284:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	7	5	theme	higher	1130:1135	arg1	level					1137:1141	higher level	1130:1141	higher level of α2,3 sialic acid residues	1130:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	6	6	from	glycome	1069:1075	arg1	present					1047:1053	present	1047:1053	present	1047:1053	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	3	7	theme	suitable	541:548	arg1	assay					550:554	suitable assay	541:554	suitable assay	541:554	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	9	8	theme	big	1504:1506	arg1	potential					1520:1528	a big application potential	1502:1528	a big application potential	1502:1528	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	5	9	theme	patients	909:916	arg1	tissue					899:904	non-tumour colon tissue	882:904	non-tumour colon tissue	882:904	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	5	9	theme	patients	909:916	arg1	tumour					871:876	tumour	871:876	tumour	871:876	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	3	10	theme	receptors	565:573	arg1	glycosylation					575:587	receptors glycosylation	565:587	receptors glycosylation	565:587	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	9	11	theme	e.g.	1533:1536	arg1	research					1548:1555	e.g. biomarker research	1533:1555	e.g. biomarker research	1533:1555	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	8	12	theme	mannose	1330:1336	arg1	structures					1338:1347	branched mannose structures	1321:1347	branched mannose structures	1321:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	0	13	theme	protein	149:155	arg1	microarray					157:166	lectin-based protein microarray	136:166	lectin-based protein microarray	136:166	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	1	14	theme	tumour	246:251	arg1	development					231:241	development	231:241	development of tumour	231:251	Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation.					
31669471	7	15	theme	sialic	1151:1156	arg1	residues					1163:1170	α2,3 sialic acid residues	1146:1170	α2,3 sialic acid residues	1146:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	1	16	from	changes	261:267	arg1	glycosylation					272:284	glycosylation	272:284	glycosylation	272:284	Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation.					
31669471	7	17	contain	possess	1122:1128	arg2	level					1182:1186	lower level	1176:1186	lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose	1176:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	17	contain	possess	1122:1128	arg1	receptors					1112:1120	receptors	1112:1120	receptors	1112:1120	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	17	contain	possess	1122:1128	arg2	level					1137:1141	higher level	1130:1141	higher level of α2,3 sialic acid residues	1130:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	5	18	theme	glycoprofile	782:793	arg1	receptors					809:817	glycoprofile IGF1 and IGF2 receptors	782:817	glycoprofile IGF1 and IGF2 receptors	782:817	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	5	19	theme	colorectal	923:932	arg1	cancer					934:939	colorectal cancer	923:939	colorectal cancer	923:939	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	6	20	from	present	1047:1053	arg1	glycome					1069:1075	the entire glycome	1058:1075	the entire glycome of membrane proteins	1058:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	1	21	theme	cell	186:189	arg1	receptors					191:199	cell receptors	186:199	cell receptors	186:199	Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation.					
31669471	6	22	theme	structures	1025:1034	arg1	partial					985:991	partial	985:991	partial	985:991	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	4	23	theme	glycosylation	687:699	arg1	pattern					701:707	the glycosylation pattern	683:707	the glycosylation pattern of receptors	683:720	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	0	24	theme	patients	95:102	arg1	tissue					85:90	colon tissue	79:90	colon tissue of patients with colorectal carcinoma	79:128	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	6	25	theme	major	1012:1016	arg1	structures					1025:1034	the major glycan structures	1008:1034	the major glycan structures with those present in the entire glycome of membrane proteins	1008:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	2	26	theme	important	434:442	arg1	ones					444:447	the most important ones	425:447	the most important ones	425:447	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	0	27	theme	colorectal	109:118	arg1	carcinoma					120:128	colorectal carcinoma	109:128	colorectal carcinoma	109:128	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	0	28	theme	factor	48:53	arg1	receptors					55:63	insulin-like growth factor receptors	28:63	insulin-like growth factor receptors	28:63	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	0	29	theme	colon	79:83	arg1	tissue					85:90	colon tissue	79:90	colon tissue of patients with colorectal carcinoma	79:128	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	0	30	theme	Sensitive	0:8	arg1	glycoprofiling					10:23	Sensitive glycoprofiling	0:23	Sensitive glycoprofiling of insulin-like growth factor receptors	0:63	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	3	31	dep	is	528:529	arg1	available					507:515	available	507:515	available	507:515	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	6	32	theme	proteins	1089:1096	arg1	glycome					1069:1075	the entire glycome	1058:1075	the entire glycome of membrane proteins	1058:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	7	33	theme	complex	1212:1218	arg1	N-glycans					1225:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	3	34	theme	small	464:468	arg1	quantities					470:479	only small quantities	459:479	only small quantities of isolated receptors	459:501	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	1	35	theme	receptors	191:199	arg1	Glycosylation					169:181	Glycosylation	169:181	Glycosylation of cell receptors	169:199	Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation.					
31669471	7	36	theme	lower	1176:1180	arg1	level					1182:1186	lower level	1176:1186	lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose	1176:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	6	37	theme	entire	1062:1067	arg1	glycome					1069:1075	the entire glycome	1058:1075	the entire glycome of membrane proteins	1058:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	2	38	theme	insulin-like	372:383	arg1	factor					392:397	insulin-like growth factor	372:397	the insulin-like growth factor (IGF) receptors	368:413	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	2	38	theme	insulin-like	372:383	arg1	IGF					400:402	IGF	400:402	IGF	400:402	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	0	39	theme	receptors	55:63	arg1	glycoprofiling					10:23	Sensitive glycoprofiling	0:23	Sensitive glycoprofiling of insulin-like growth factor receptors	0:63	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	9	40	contain	has	1498:1500	arg2	potential					1520:1528	a big application potential	1502:1528	a big application potential	1502:1528	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	9	40	contain	has	1498:1500	arg1	method					1453:1458	The described method	1439:1458	The described method enabling glycan analysis of receptors	1439:1496	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	3	41	theme	receptors	493:501	arg1	quantities					470:479	only small quantities	459:479	only small quantities of isolated receptors	459:501	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	9	42	theme	described	1443:1451	arg1	method					1453:1458	The described method	1439:1458	The described method enabling glycan analysis of receptors	1439:1496	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	4	43	theme	reverse-phase	629:641	arg1	method					662:667	a lectin-based reverse-phase protein microarray method	614:667	a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations	614:753	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	7	44	theme	mannose	1248:1254	arg1	level					1182:1186	lower level	1176:1186	lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose	1176:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	44	theme	mannose	1248:1254	arg1	level					1137:1141	higher level	1130:1141	higher level of α2,3 sialic acid residues	1130:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	45	theme	α2,3	1146:1149	arg1	residues					1163:1170	α2,3 sialic acid residues	1146:1170	α2,3 sialic acid residues	1146:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	2	46	theme	factor	392:397	arg1	receptors					405:413	the insulin-like growth factor (IGF) receptors	368:413	the insulin-like growth factor (IGF) receptors	368:413	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	3	47	theme	assay	550:554	arg1	need					533:536	a need	531:536	a need of suitable assay to study receptors glycosylation	531:587	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	9	48	theme	receptors	1488:1496	arg1	analysis					1476:1483	glycan analysis	1469:1483	glycan analysis of receptors	1469:1496	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	5	49	theme	colon	893:897	arg1	tissue					899:904	non-tumour colon tissue	882:904	non-tumour colon tissue	882:904	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	7	50	theme	residues	1163:1170	arg1	level					1182:1186	lower level	1176:1186	lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose	1176:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	50	theme	residues	1163:1170	arg1	level					1137:1141	higher level	1130:1141	higher level of α2,3 sialic acid residues	1130:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	2	51	theme	growth	349:354	arg1	factors					356:362	growth factors	349:362	growth factors	349:362	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	9	52	theme	application	1508:1518	arg1	potential					1520:1528	a big application potential	1502:1528	a big application potential	1502:1528	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	0	53	theme	lectin-based	136:147	arg1	microarray					157:166	lectin-based protein microarray	136:166	lectin-based protein microarray	136:166	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	0	54	with	patients	95:102	arg1	carcinoma					120:128	colorectal carcinoma	109:128	colorectal carcinoma	109:128	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	4	55	theme	microarray	651:660	arg1	method					662:667	a lectin-based reverse-phase protein microarray method	614:667	a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations	614:753	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	9	56	theme	biomarker	1538:1546	arg1	research					1548:1555	e.g. biomarker research	1533:1555	e.g. biomarker research	1533:1555	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31669471	6	57	attach	present	1047:1053	arg2	those					1041:1045	those	1041:1045	those	1041:1045	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	6	57	attach	present	1047:1053	arg1	glycome					1069:1075	the entire glycome	1058:1075	the entire glycome of membrane proteins	1058:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	8	58	theme	branched	1321:1328	arg1	structures					1338:1347	branched mannose structures	1321:1347	branched mannose structures	1321:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	1	59	gly	Glycosylation	169:181	arg1	receptors					191:199	cell receptors	186:199	cell receptors	186:199	Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation.					
31669471	8	60	theme	structures	1338:1347	arg1	levels					1294:1299	Increased levels	1284:1299	Increased levels of fucosylation and branched mannose structures	1284:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	8	61	attach	derived	1381:1387	arg2	receptors					1371:1379	both receptors	1366:1379	both receptors derived from tumour tissue	1366:1406	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	8	61	attach	derived	1381:1387	arg1	tissue					1401:1406	tumour tissue	1394:1406	tumour tissue	1394:1406	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	2	62	dep	bind	344:347	arg1	ones					444:447	the most important ones	425:447	the most important ones	425:447	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	7	63	theme	high-mannose	1259:1270	arg1	structures					1272:1281	high-mannose structures	1259:1281	high-mannose structures	1259:1281	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	2	64	theme	Cell	287:290	arg1	growth					292:297	Cell growth	287:297	Cell growth	287:297	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	6	65	with	structures	1025:1034	arg1	those					1041:1045	those	1041:1045	those	1041:1045	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	4	66	theme	receptors	712:720	arg1	pattern					701:707	the glycosylation pattern	683:707	the glycosylation pattern of receptors	683:720	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	7	67	theme	terminal	1239:1246	arg1	mannose					1248:1254	terminal mannose	1239:1254	terminal mannose	1239:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	5	68	theme	IGF1	795:798	arg1	receptors					809:817	glycoprofile IGF1 and IGF2 receptors	782:817	glycoprofile IGF1 and IGF2 receptors	782:817	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	4	69	theme	picomolar	725:733	arg1	concentrations					740:753	picomolar (pM) concentrations	725:753	picomolar (pM) concentrations	725:753	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	4	69	theme	picomolar	725:733	arg1	pM					736:737	pM	736:737	pM	736:737	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	2	70	theme	receptors	328:336	arg1	activation					314:323	the activation	310:323	the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones	310:447	Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones.					
31669471	6	71	theme	glycan	1018:1023	arg1	structures					1025:1034	the major glycan structures	1008:1034	the major glycan structures with those present in the entire glycome of membrane proteins	1008:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	8	72	theme	tumour	1394:1399	arg1	tissue					1401:1406	tumour tissue	1394:1406	tumour tissue	1394:1406	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	7	73	theme	type	1220:1223	arg1	N-glycans					1225:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	8	74	theme	fucosylation	1304:1315	arg1	levels					1294:1299	Increased levels	1284:1299	Increased levels of fucosylation and branched mannose structures	1284:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	5	75	theme	solubilised	827:837	arg1	proteins					848:855	the solubilised membrane proteins	823:855	the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer	823:939	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	8	76	theme	non-tumour	1420:1429	arg1	tissue					1431:1436	non-tumour tissue	1420:1436	non-tumour tissue	1420:1436	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	0	77	theme	insulin-like	28:39	arg1	factor					48:53	insulin-like growth factor	28:53	insulin-like growth factor receptors	28:63	Sensitive glycoprofiling of insulin-like growth factor receptors isolated from colon tissue of patients with colorectal carcinoma using lectin-based protein microarray.					
31669471	5	78	theme	membrane	839:846	arg1	proteins					848:855	the solubilised membrane proteins	823:855	the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer	823:939	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	6	79	theme	membrane	1080:1087	arg1	proteins					1089:1096	membrane proteins	1080:1096	membrane proteins	1080:1096	We found that common to both receptors was partial overlapping of the major glycan structures with those present in the entire glycome of membrane proteins.					
31669471	3	80	theme	isolated	484:491	arg1	receptors					493:501	isolated receptors	484:501	isolated receptors	484:501	Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study receptors glycosylation.					
31669471	5	81	theme	IGF2	804:807	arg1	receptors					809:817	glycoprofile IGF1 and IGF2 receptors	782:817	glycoprofile IGF1 and IGF2 receptors	782:817	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	8	82	located	observed	1354:1361	arg1	receptors					1371:1379	both receptors	1366:1379	both receptors derived from tumour tissue	1366:1406	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	8	82	located	observed	1354:1361	arg2	levels					1294:1299	Increased levels	1284:1299	Increased levels of fucosylation and branched mannose structures	1284:1347	Increased levels of fucosylation and branched mannose structures were observed in both receptors derived from tumour tissue compared to non-tumour tissue.					
31669471	7	83	theme	N-glycans	1225:1233	arg1	level					1182:1186	lower level	1176:1186	lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose	1176:1254	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	83	theme	N-glycans	1225:1233	arg1	level					1137:1141	higher level	1130:1141	higher level of α2,3 sialic acid residues	1130:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	7	84	theme	acid	1158:1161	arg1	residues					1163:1170	α2,3 sialic acid residues	1146:1170	α2,3 sialic acid residues	1146:1170	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	5	85	with	patients	909:916	arg1	cancer					934:939	colorectal cancer	923:939	colorectal cancer	923:939	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	4	86	theme	lectin-based	616:627	arg1	method					662:667	a lectin-based reverse-phase protein microarray method	614:667	a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations	614:753	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	5	87	theme	non-tumour	882:891	arg1	tissue					899:904	non-tumour colon tissue	882:904	non-tumour colon tissue	882:904	The method was applied to glycoprofile IGF1 and IGF2 receptors and the solubilised membrane proteins isolated from tumour and non-tumour colon tissue of patients with colorectal cancer.					
31669471	4	88	theme	protein	643:649	arg1	method					662:667	a lectin-based reverse-phase protein microarray method	614:667	a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations	614:753	Therefore, we developed a lectin-based reverse-phase protein microarray method for screening the glycosylation pattern of receptors in picomolar (pM) concentrations.					
31669471	7	89	theme	tri-/tetra-antennary	1191:1210	arg1	N-glycans					1225:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	tri-/tetra-antennary complex type N-glycans	1191:1233	In contrast, receptors possess higher level of α2,3 sialic acid residues and lower level of tri-/tetra-antennary complex type N-glycans and terminal mannose in high-mannose structures.					
31669471	9	90	theme	glycan	1469:1474	arg1	analysis					1476:1483	glycan analysis	1469:1483	glycan analysis of receptors	1469:1496	The described method enabling glycan analysis of receptors has a big application potential in e.g. biomarker research, biology and diagnostics.					
31160824	5	0	theme	structural	630:639	arg1	techniques					653:662	transcriptomic, biochemical, structural and genetic techniques	601:662	transcriptomic, biochemical, structural and genetic techniques	601:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	1	1	theme	carbon	157:162	arg1	Glycans					135:141	Glycans	135:141	Glycans	135:141	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	1	1	theme	carbon	157:162	arg1	sources					164:170	the major carbon sources	147:170	the major carbon sources available to the human colonic microbiota	147:212	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	5	2	theme	CNG	733:735	arg1	degradation					737:747	CNG degradation	733:747	CNG degradation	733:747	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	7	3	theme	classes	1131:1137	arg1	degradation					1110:1120	the degradation	1106:1120	the degradation of other classes of glycan	1106:1147	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	4	4	theme	complex	489:495	arg1	CNGs					508:511	CNGs	508:511	CNGs	508:511	have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins.					
31160824	4	4	theme	complex	489:495	arg1	nutrients					517:525	nutrients	517:525	nutrients	517:525	have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins.					
31160824	4	4	theme	complex	489:495	arg1	N-glycans					497:505	complex N-glycans	489:505	complex N-glycans (CNGs)	489:512	have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins.					
31160824	4	4	theme	complex	489:495	arg1	those					538:542	those	538:542	those	538:542	have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins.					
31160824	8	5	located	found	1287:1291	arg1	niche					1311:1315	the intestinal niche	1296:1315	the intestinal niche	1296:1315	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	8	5	located	found	1287:1291	arg2	variants					1257:1264	different structural variants	1236:1264	different structural variants of CNGs	1236:1272	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	8	5	located	found	1287:1291	arg2	myriad					1229:1234	the myriad different structural variants of CNGs likely to be found in the intestinal niche	1225:1315	the myriad different structural variants of CNGs likely to be found in the intestinal niche	1225:1315	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	5	6	theme	Detailed	566:573	arg1	studies					587:593	Detailed mechanistic studies	566:593	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques	566:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	2	7	dep	both	281:284	arg1	dietary					286:292	dietary	286:292	dietary	286:292	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	8	located	found	252:256	arg2	proteins					239:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	8	located	found	252:256	arg1	gut					271:273	the human gut	261:273	the human gut	261:273	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	0	9	theme	multiple	99:106	arg1	loci					129:132	multiple co-regulated genetic loci	99:132	multiple co-regulated genetic loci	99:132	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	5	10	theme	genetic	645:651	arg1	techniques					653:662	transcriptomic, biochemical, structural and genetic techniques	601:662	transcriptomic, biochemical, structural and genetic techniques	601:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	5	11	theme	mechanistic	575:585	arg1	studies					587:593	Detailed mechanistic studies	566:593	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques	566:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	8	12	with	Bt	1194:1195	arg1	capacity					1206:1213	the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche	1202:1315	the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche	1202:1315	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	8	13	theme	different	1236:1244	arg1	variants					1257:1264	different structural variants	1236:1264	different structural variants of CNGs	1236:1272	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	0	14	theme	genetic	121:127	arg1	loci					129:132	multiple co-regulated genetic loci	99:132	multiple co-regulated genetic loci	99:132	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	6	15	theme	different	912:920	arg1	families					922:929	different families	912:929	different families	912:929	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	6	15	theme	different	912:920	arg1	activity					974:981	a CNG-specific endo-glycosidase activity	942:981	a CNG-specific endo-glycosidase activity	942:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	5	16	theme	transcriptomic	601:614	arg1	techniques					653:662	transcriptomic, biochemical, structural and genetic techniques	601:662	transcriptomic, biochemical, structural and genetic techniques	601:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	6	17	theme	enzymatic	794:802	arg1	apparatus					804:812	an extensive enzymatic apparatus	781:812	an extensive enzymatic apparatus encoded by multiple non-adjacent loci	781:850	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	1	18	theme	available	172:180	arg1	Glycans					135:141	Glycans	135:141	Glycans	135:141	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	1	18	theme	available	172:180	arg1	sources					164:170	the major carbon sources	147:170	the major carbon sources available to the human colonic microbiota	147:212	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	0	19	theme	co-regulated	108:119	arg1	loci					129:132	multiple co-regulated genetic loci	99:132	multiple co-regulated genetic loci	99:132	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	6	20	theme	extensive	784:792	arg1	apparatus					804:812	an extensive enzymatic apparatus	781:812	an extensive enzymatic apparatus encoded by multiple non-adjacent loci	781:850	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	6	21	from	families	922:929	arg1	enzymes					899:905	19 different carbohydrate-active enzymes	866:905	19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity	866:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	0	22	theme	N-glycan	8:15	arg1	breakdown					17:25	Complex N-glycan breakdown	0:25	Complex N-glycan breakdown by gut Bacteroides	0:44	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	5	23	theme	biochemical	617:627	arg1	techniques					653:662	transcriptomic, biochemical, structural and genetic techniques	601:662	transcriptomic, biochemical, structural and genetic techniques	601:662	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	0	24	theme	Complex	0:6	arg1	breakdown					17:25	Complex N-glycan breakdown	0:25	Complex N-glycan breakdown by gut Bacteroides	0:44	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	6	25	theme	multiple	825:832	arg1	loci					847:850	multiple non-adjacent loci	825:850	multiple non-adjacent loci	825:850	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	8	26	theme	intestinal	1300:1309	arg1	niche					1311:1315	the intestinal niche	1296:1315	the intestinal niche	1296:1315	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	1	27	theme	human	189:193	arg1	microbiota					203:212	the human colonic microbiota	185:212	the human colonic microbiota	185:212	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	7	28	theme	other	1125:1129	arg1	classes					1131:1137	other classes	1125:1137	other classes of glycan	1125:1147	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	7	29	theme	CNG	997:999	arg1	degradation					1001:1011	CNG degradation	997:1011	CNG degradation	997:1011	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	3	30	theme	gut	439:441	arg1	spp					455:457	many mutualistic gut Bacteroides spp	422:457	many mutualistic gut Bacteroides spp	422:457	Here, we show that many mutualistic gut Bacteroides spp.					
31160824	7	31	theme	carbohydrate-active	1038:1056	arg1	enzymes					1058:1064	carbohydrate-active enzymes	1038:1064	carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan	1038:1147	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	5	32	theme	Bacteroides	695:705	arg1	Bt					725:726	Bt	725:726	Bt	725:726	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	5	32	theme	Bacteroides	695:705	arg1	thetaiotaomicron					707:722	Bacteroides thetaiotaomicron	695:722	Bacteroides thetaiotaomicron (Bt)	695:727	Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation.					
31160824	6	33	theme	carbohydrate-active	879:897	arg1	enzymes					899:905	19 different carbohydrate-active enzymes	866:905	19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity	866:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	3	34	theme	Bacteroides	443:453	arg1	spp					455:457	many mutualistic gut Bacteroides spp	422:457	many mutualistic gut Bacteroides spp	422:457	Here, we show that many mutualistic gut Bacteroides spp.					
31160824	8	35	theme	CNGs	1269:1272	arg1	variants					1257:1264	different structural variants	1236:1264	different structural variants of CNGs	1236:1272	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	6	36	theme	different	869:877	arg1	enzymes					899:905	19 different carbohydrate-active enzymes	866:905	19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity	866:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	3	37	theme	many	422:425	arg1	spp					455:457	many mutualistic gut Bacteroides spp	422:457	many mutualistic gut Bacteroides spp	422:457	Here, we show that many mutualistic gut Bacteroides spp.					
31160824	0	38	theme	gut	30:32	arg1	Bacteroides					34:44	gut Bacteroides	30:44	gut Bacteroides	30:44	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	6	39	theme	breakdown	754:762	arg1	process					764:770	The breakdown process	750:770	The breakdown process	750:770	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	2	40	theme	high	390:393	arg1	levels					395:400	high levels	390:400	high levels	390:400	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	41	gly	N-glycosylated	224:237	arg1	proteins					239:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	3	42	dep	show	412:415	arg1	spp					455:457	many mutualistic gut Bacteroides spp	422:457	many mutualistic gut Bacteroides spp	422:457	Here, we show that many mutualistic gut Bacteroides spp.					
31160824	8	43	theme	complex	1155:1161	arg1	apparatus					1175:1183	This complex and diverse apparatus	1150:1183	This complex and diverse apparatus	1150:1183	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	2	44	theme	Numerous	215:222	arg1	proteins					239:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	45	theme	N-glycosylated	224:237	arg1	proteins					239:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins	215:246	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	7	46	theme	enzymes	1058:1064	arg1	activity					1026:1033	the activity	1022:1033	the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan	1022:1147	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	6	47	theme	endo-glycosidase	957:972	arg1	activity					974:981	a CNG-specific endo-glycosidase activity	942:981	a CNG-specific endo-glycosidase activity	942:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	8	48	theme	diverse	1167:1173	arg1	apparatus					1175:1183	This complex and diverse apparatus	1150:1183	This complex and diverse apparatus	1150:1183	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	6	49	theme	non-adjacent	834:845	arg1	loci					847:850	multiple non-adjacent loci	825:850	multiple non-adjacent loci	825:850	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	2	50	theme	human	265:269	arg1	gut					271:273	the human gut	261:273	the human gut	261:273	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	51	dep	sources	303:309	arg1	both					281:284	both	281:284	both	281:284	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	2	51	dep	sources	303:309	arg1	host					298:301	host	298:301	host	298:301	Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels.					
31160824	7	52	theme	glycan	1142:1147	arg1	classes					1131:1137	other classes	1125:1137	other classes of glycan	1125:1147	Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan.					
31160824	0	53	theme	enzymatic	68:76	arg1	apparatus					78:86	an extensive enzymatic apparatus	55:86	an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci	55:132	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	3	54	theme	mutualistic	427:437	arg1	spp					455:457	many mutualistic gut Bacteroides spp	422:457	many mutualistic gut Bacteroides spp	422:457	Here, we show that many mutualistic gut Bacteroides spp.					
31160824	1	55	theme	colonic	195:201	arg1	microbiota					203:212	the human colonic microbiota	185:212	the human colonic microbiota	185:212	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	0	56	theme	extensive	58:66	arg1	apparatus					78:86	an extensive enzymatic apparatus	55:86	an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci	55:132	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.					
31160824	6	57	theme	CNG-specific	944:955	arg1	activity					974:981	a CNG-specific endo-glycosidase activity	942:981	a CNG-specific endo-glycosidase activity	942:981	The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity.					
31160824	8	58	theme	structural	1246:1255	arg1	variants					1257:1264	different structural variants	1236:1264	different structural variants of CNGs	1236:1272	This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.					
31160824	1	59	theme	major	151:155	arg1	Glycans					135:141	Glycans	135:141	Glycans	135:141	Glycans are the major carbon sources available to the human colonic microbiota.					
31160824	1	59	theme	major	151:155	arg1	sources					164:170	the major carbon sources	147:170	the major carbon sources available to the human colonic microbiota	147:212	Glycans are the major carbon sources available to the human colonic microbiota.					
31572782	3	0	theme	N-glycan	329:336	arg1	composition					338:348	the N-glycan composition	325:348	the N-glycan composition in intact glycoproteins and under physiological conditions	325:407	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	4	1	theme	human	529:533	arg1	cells					543:547	human HEK 293 cells	529:547	human HEK 293 cells	529:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	2	theme	glycan	610:615	arg1	epitopes					617:624	specific glycan epitopes	601:624	specific glycan epitopes	601:624	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	5	3	theme	Chemical	627:634	arg1	shifts					636:641	Chemical shifts	627:641	Chemical shifts	627:641	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	5	4	from	shifts	636:641	arg1	line-broadening					673:687	the signal line-broadening	662:687	the signal line-broadening	662:687	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	4	5	theme	specific	601:608	arg1	epitopes					617:624	specific glycan epitopes	601:624	specific glycan epitopes	601:624	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	5	6	theme	structural	759:768	arg1	able					795:798	able	795:798	able	795:798	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	5	6	theme	structural	759:768	arg1	model					770:774	a structural model	757:774	a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface	757:893	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	6	7	theme	solvent	944:950	arg1	accessibility					952:964	a large solvent accessibility	936:964	a large solvent accessibility	936:964	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	7	8	theme	new	1146:1148	arg1	avenues					1150:1156	new avenues	1146:1156	new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans	1146:1284	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	6	9	theme	complex	905:911	arg1	N-glycans					918:926	complex type N-glycans	905:926	complex type N-glycans	905:926	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	3	10	theme	physiological	384:396	arg1	conditions					398:407	physiological conditions	384:407	physiological conditions	384:407	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	6	11	theme	large	938:942	arg1	accessibility					952:964	a large solvent accessibility	936:964	a large solvent accessibility	936:964	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	4	12	theme	epitopes	617:624	arg1	presence					566:573	presence	566:573	presence	566:573	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	12	theme	epitopes	617:624	arg1	abundance					588:596	relative abundance	579:596	relative abundance	579:596	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	5	13	theme	intramolecular	812:825	arg1	interactions					827:838	intramolecular interactions	812:838	intramolecular interactions between high-mannose N-glycans	812:869	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	5	14	theme	FcεRIα	779:784	arg1	able					795:798	able	795:798	able	795:798	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	5	14	theme	FcεRIα	779:784	arg1	model					770:774	a structural model	757:774	a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface	757:893	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	7	15	theme	specific	1179:1186	arg1	epitopes					1197:1204	specific N-glycan epitopes	1179:1204	specific N-glycan epitopes	1179:1204	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	5	16	theme	high-mannose	848:859	arg1	N-glycans					861:869	high-mannose N-glycans	848:869	high-mannose N-glycans	848:869	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	5	17	theme	signal	666:671	arg1	line-broadening					673:687	the signal line-broadening	662:687	the signal line-broadening	662:687	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	1	18	theme	Protein	81:87	arg1	N-glycosylation					89:103	Protein N-glycosylation	81:103	Protein N-glycosylation	81:103	Protein N-glycosylation stands out for its intrinsic and functionally related heterogeneity.					
31572782	0	19	theme	Glycoprofile	0:11	arg1	Analysis					13:20	Glycoprofile Analysis	0:20	Glycoprofile Analysis of an Intact Glycoprotein As	0:49	Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy.					
31572782	4	20	theme	relative	579:586	arg1	abundance					588:596	relative abundance	579:596	relative abundance	579:596	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	21	theme	employed	414:421	arg1	methodology					423:433	The employed methodology	410:433	The employed methodology	410:433	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	22	theme	IgE	480:482	arg1	FcεRIα					508:513	FcεRIα	508:513	FcεRIα	508:513	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	22	theme	IgE	480:482	arg1	receptor					498:505	the IgE high-affinity receptor	476:505	the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells	476:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	7	23	theme	epitopes	1197:1204	arg1	detection					1166:1174	the detection	1162:1174	the detection of specific N-glycan epitopes	1162:1204	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	5	24	theme	protein	879:885	arg1	surface					887:893	the protein surface	875:893	the protein surface	875:893	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	3	25	theme	NMR-based	293:301	arg1	strategy					303:310	an NMR-based strategy	290:310	an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions	290:407	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	4	26	theme	high-affinity	484:496	arg1	FcεRIα					508:513	FcεRIα	508:513	FcεRIα	508:513	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	26	theme	high-affinity	484:496	arg1	receptor					498:505	the IgE high-affinity receptor	476:505	the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells	476:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	7	27	theme	N-glycan	1188:1195	arg1	epitopes					1197:1204	specific N-glycan epitopes	1179:1204	specific N-glycan epitopes	1179:1204	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	0	28	theme	Glycoprotein	35:46	arg1	Analysis					13:20	Glycoprofile Analysis	0:20	Glycoprofile Analysis of an Intact Glycoprotein As	0:49	Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy.					
31572782	6	29	theme	interaction	999:1009	arg1	role					991:994	a functional role	978:994	a functional role	978:994	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	6	29	theme	interaction	999:1009	arg1	sites					1011:1015	interaction sites	999:1015	interaction sites for receptors	999:1029	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	5	30	dep	native	701:706	arg1	states					725:730	states	725:730	states	725:730	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	2	31	theme	biomedical	186:195	arg1	interest					197:204	its biomedical interest	182:204	its biomedical interest	182:204	Despite its biomedical interest, Glycoprofile analysis still remains a major scientific challenge.					
31572782	2	32	theme	scientific	251:260	arg1	challenge					262:270	a major scientific challenge	243:270	a major scientific challenge	243:270	Despite its biomedical interest, Glycoprofile analysis still remains a major scientific challenge.					
31572782	0	33	theme	Intact	28:33	arg1	Glycoprotein					35:46	an Intact Glycoprotein	25:46	an Intact Glycoprotein As	25:49	Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy.					
31572782	3	34	gly	glycoproteins	360:372	arg1	glycoproteins					360:372	intact glycoproteins	353:372	intact glycoproteins	353:372	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	2	35	theme	major	245:249	arg1	challenge					262:270	a major scientific challenge	243:270	a major scientific challenge	243:270	Despite its biomedical interest, Glycoprofile analysis still remains a major scientific challenge.					
31572782	7	36	theme	glycoprotein-receptor	1229:1249	arg1	interactions					1251:1262	glycoprotein-receptor interactions	1229:1262	glycoprotein-receptor interactions mediated by N-glycans	1229:1284	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	4	37	theme	receptor	498:505	arg1	pattern					465:471	the glycan pattern	454:471	the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells	454:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	6	38	theme	type	913:916	arg1	N-glycans					918:926	complex type N-glycans	905:926	complex type N-glycans	905:926	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	6	39	theme	functional	980:989	arg1	role					991:994	a functional role	978:994	a functional role	978:994	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	6	39	theme	functional	980:989	arg1	sites					1011:1015	interaction sites	999:1015	interaction sites for receptors	999:1029	In turn, complex type N-glycans reflect a large solvent accessibility, suggesting a functional role as interaction sites for receptors.					
31572782	4	40	dep	presence	566:573	arg1	the					562:564	the	562:564	the	562:564	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	4	41	theme	glycan	458:463	arg1	pattern					465:471	the glycan pattern	454:471	the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells	454:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	7	42	theme	intact	1056:1061	arg1	FcεRIα					1063:1068	intact FcεRIα	1056:1068	intact FcεRIα	1056:1068	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	7	43	theme	lectin	1078:1083	arg1	hGal3					1085:1089	the lectin hGal3	1074:1089	the lectin hGal3	1074:1089	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31572782	3	44	from	composition	338:348	arg1	glycoproteins					360:372	intact glycoproteins	353:372	intact glycoproteins	353:372	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	1	45	theme	intrinsic	124:132	arg1	heterogeneity					159:171	its intrinsic and functionally related heterogeneity	120:171	its intrinsic and functionally related heterogeneity	120:171	Protein N-glycosylation stands out for its intrinsic and functionally related heterogeneity.					
31572782	2	46	theme	Glycoprofile	207:218	arg1	analysis					220:227	Glycoprofile analysis	207:227	Glycoprofile analysis	207:227	Despite its biomedical interest, Glycoprofile analysis still remains a major scientific challenge.					
31572782	5	47	from	differences	647:657	arg1	line-broadening					673:687	the signal line-broadening	662:687	the signal line-broadening	662:687	Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the protein surface.					
31572782	0	48	theme	NMR	63:65	arg1	Spectroscopy					67:78	NMR Spectroscopy	63:78	NMR Spectroscopy	63:78	Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy.					
31572782	3	49	theme	intact	353:358	arg1	glycoproteins					360:372	intact glycoproteins	353:372	intact glycoproteins	353:372	Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions.					
31572782	4	50	theme	HEK	535:537	arg1	cells					543:547	human HEK 293 cells	529:547	human HEK 293 cells	529:547	The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes.					
31572782	1	51	theme	related	151:157	arg1	heterogeneity					159:171	its intrinsic and functionally related heterogeneity	120:171	its intrinsic and functionally related heterogeneity	120:171	Protein N-glycosylation stands out for its intrinsic and functionally related heterogeneity.					
31572782	7	52	theme	interactions	1251:1262	arg1	studies					1218:1224	the studies	1214:1224	the studies of glycoprotein-receptor interactions mediated by N-glycans	1214:1284	The interaction between intact FcεRIα and the lectin hGal3, also studied here, confirms this hypothesis and opens new avenues for the detection of specific N-glycan epitopes and for the studies of glycoprotein-receptor interactions mediated by N-glycans.					
31747272	4	0	from	composition	527:537	arg1	terms					577:581	terms	577:581	terms of DP	577:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	10	1	theme	Bifidobacterial	1306:1320	arg1	strains					1322:1328	Bifidobacterial strains	1306:1328	Bifidobacterial strains	1306:1328	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	11	2	theme	genome-encoded	1770:1783	arg1	transporters					1785:1796	genome-encoded transporters	1770:1796	genome-encoded transporters	1770:1796	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	6	3	theme	probiotic	879:887	arg1	strains					889:895	the probiotic strains	875:895	the probiotic strains	875:895	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	5	4	theme	single	664:669	arg1	strains					682:688	13 (single) probiotic strains	660:688	13 (single) probiotic strains	660:688	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	2	5	theme	beneficial	327:336	arg1	bacteria					342:349	beneficial gut bacteria	327:349	beneficial gut bacteria	327:349	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	11	6	theme	carbohydrate-active	1802:1820	arg1	enzymes					1822:1828	carbohydrate-active enzymes	1802:1828	carbohydrate-active enzymes	1802:1828	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	10	7	theme	infantis	1480:1487	arg1	20088					1493:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	0	8	theme	Structural	0:9	arg1	Identity					11:18	Structural Identity	0:18	Structural Identity of Galactooligosaccharide Molecules	0:54	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	12	9	theme	bacterial	1922:1930	arg1	strains					1932:1938	probiotic bacterial strains	1912:1938	probiotic bacterial strains with GOS compounds that specifically stimulate their growth	1912:1998	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	6	10	theme	OD600nm	936:942	arg1	OD600nm					936:942	OD600nm	936:942	OD600nm	936:942	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	6	10	theme	OD600nm	936:942	arg1	%					931:931	30 and 100%	921:931	30 and 100% of OD600nm relative to positive controls with glucose	921:985	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	11	11	theme	genomic	1686:1692	arg1	information					1694:1704	their genomic information	1680:1704	their genomic information	1680:1704	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	5	12	theme	bacteria	697:704	arg1	strains					682:688	13 (single) probiotic strains	660:688	13 (single) probiotic strains	660:688	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	13	13	theme	pharmacy/medicine	2068:2084	arg1	applications					2086:2097	food/feed and/or pharmacy/medicine applications	2051:2097	food/feed and/or pharmacy/medicine applications	2051:2097	Such synbiotic combinations may be of interest in food/feed and/or pharmacy/medicine applications.					
31747272	2	14	theme	bacteria	342:349	arg1	growth					317:322	growth	317:322	growth of beneficial gut bacteria	317:349	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	11	15	theme	enzymes	1822:1828	arg1	presence					1758:1765	the presence	1754:1765	the presence of genome-encoded transporters and carbohydrate-active enzymes	1754:1828	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	7	16	theme	mixture	1030:1036	arg1	components					1007:1016	the components	1003:1016	the components of the pGOS mixture that remain after growth	1003:1061	By identifying the components of the pGOS mixture that remain after growth, we showed that strains varied in their consumption of specific GOS compounds.					
31747272	4	17	from	terms	577:581	arg1	composition					527:537	the composition	523:537	the composition of several commercial GOS mixtures in terms of DP	523:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	8	18	theme	GOS	1180:1182	arg1	pool					1188:1191	the GOS DP2 pool	1176:1191	the GOS DP2 pool	1176:1191	All strains commonly used most of the GOS DP2 pool.					
31747272	5	19	theme	probiotic	672:680	arg1	strains					682:688	13 (single) probiotic strains	660:688	13 (single) probiotic strains	660:688	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	9	20	theme	salivarius	1208:1217	arg1	W57					1219:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	11	21	theme	bacterial	1640:1648	arg1	profiles					1666:1673	these bacterial GOS consumption profiles	1634:1673	these bacterial GOS consumption profiles	1634:1673	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	13	22	theme	food/feed	2051:2059	arg1	applications					2086:2097	food/feed and/or pharmacy/medicine applications	2051:2097	food/feed and/or pharmacy/medicine applications	2051:2097	Such synbiotic combinations may be of interest in food/feed and/or pharmacy/medicine applications.					
31747272	3	23	with	compounds	375:383	arg1	linkages					467:474	diverse glycosidic linkages	448:474	diverse glycosidic linkages	448:474	GOS consists mainly of compounds with a degree of polymerization (DP) varying from 2-8 and with diverse glycosidic linkages.					
31747272	3	23	with	compounds	375:383	arg1	degree					392:397	a degree	390:397	a degree of polymerization (DP) varying from 2-8	390:437	GOS consists mainly of compounds with a degree of polymerization (DP) varying from 2-8 and with diverse glycosidic linkages.					
31747272	0	24	theme	Single	80:85	arg1	Cultures					87:94	Single Cultures	80:94	Single Cultures of Probiotic Bacterial Strains	80:125	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	5	25	link	GOS-derived	788:798	arg1	sample					800:805	a Vivinal GOS-derived sample	778:805	a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS)	778:859	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	10	26	dep	Lactobacillus	1429:1441	arg1	acidophilus					1443:1453	acidophilus	1443:1453	acidophilus	1443:1453	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	9	27	theme	branched	1245:1252	arg1	-d-Glc					1298:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	12	28	theme	detailed	1837:1844	arg1	insights					1846:1853	These detailed insights	1831:1853	These detailed insights	1831:1853	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	11	29	theme	consumption	1654:1664	arg1	profiles					1666:1673	these bacterial GOS consumption profiles	1634:1673	these bacterial GOS consumption profiles	1634:1673	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	0	30	theme	Bacterial	109:117	arg1	Strains					119:125	Probiotic Bacterial Strains	99:125	Probiotic Bacterial Strains	99:125	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	9	31	theme	β-d-Galp-	1263:1271	arg1	-d-Glc					1298:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	1	32	with	reaction	193:200	arg1	lactose					207:213	lactose	207:213	lactose	207:213	Various β-galactosidase enzymes catalyze the trans-glycosylation reaction with lactose.					
31747272	13	33	theme	Such	2001:2004	arg1	combinations					2016:2027	Such synbiotic combinations	2001:2027	Such synbiotic combinations	2001:2027	Such synbiotic combinations may be of interest in food/feed and/or pharmacy/medicine applications.					
31747272	4	34	from	mixtures	565:572	arg1	terms					577:581	terms	577:581	terms of DP	577:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	3	35	theme	diverse	448:454	arg1	linkages					467:474	diverse glycosidic linkages	448:474	diverse glycosidic linkages	448:474	GOS consists mainly of compounds with a degree of polymerization (DP) varying from 2-8 and with diverse glycosidic linkages.					
31747272	5	36	theme	GOS-derived	788:798	arg1	sample					800:805	a Vivinal GOS-derived sample	778:805	a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS)	778:859	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	2	37	theme	infant	287:292	arg1	nutrition					294:302	infant nutrition	287:302	infant nutrition	287:302	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	10	38	theme	DSM	1418:1420	arg1	20091					1422:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	7	39	theme	GOS	1127:1129	arg1	compounds					1131:1139	specific GOS compounds	1118:1139	specific GOS compounds	1118:1139	By identifying the components of the pGOS mixture that remain after growth, we showed that strains varied in their consumption of specific GOS compounds.					
31747272	1	40	theme	Various	128:134	arg1	enzymes					152:158	Various β-galactosidase enzymes	128:158	Various β-galactosidase enzymes	128:158	Various β-galactosidase enzymes catalyze the trans-glycosylation reaction with lactose.					
31747272	10	41	theme	Bifidobacterium	1396:1410	arg1	20091					1422:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	2	42	theme	resulting	220:228	arg1	mixtures					259:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	0	43	theme	Molecules	46:54	arg1	Identity					11:18	Structural Identity	0:18	Structural Identity of Galactooligosaccharide Molecules	0:54	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	9	44	dep	→	1275:1275	arg1	-[β-d-Galp-					1279:1289	-[β-d-Galp-	1279:1289	1 → 4)-[β-d-Galp-(1 → 2)	1273:1296	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	9	44	dep	→	1275:1275	arg1	4					1277:1277	4	1277:1277	4	1277:1277	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	4	45	theme	GOS	561:563	arg1	mixtures					565:572	several commercial GOS mixtures	542:572	several commercial GOS mixtures in terms of DP	542:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	10	46	theme	different	1579:1587	arg1	structures					1589:1598	the 40 different structures	1572:1598	the 40 different structures identified in pGOS	1572:1617	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	10	47	theme	Bifidobacterium	1464:1478	arg1	20088					1493:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	11	48	theme	transporters	1785:1796	arg1	presence					1758:1765	the presence	1754:1765	the presence of genome-encoded transporters and carbohydrate-active enzymes	1754:1828	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	4	49	theme	individual	624:633	arg1	compounds					635:643	the individual compounds	620:643	the individual compounds	620:643	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	13	50	from	interest	2039:2046	arg1	applications					2086:2097	food/feed and/or pharmacy/medicine applications	2051:2097	food/feed and/or pharmacy/medicine applications	2051:2097	Such synbiotic combinations may be of interest in food/feed and/or pharmacy/medicine applications.					
31747272	2	51	theme	gut	338:340	arg1	bacteria					342:349	beneficial gut bacteria	327:349	beneficial gut bacteria	327:349	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	4	52	theme	structural	597:606	arg1	identity					608:615	the structural identity	593:615	the structural identity of the individual compounds	593:643	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	10	53	theme	DSM	1489:1491	arg1	20088					1493:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterium infantis DSM 20088	1464:1497	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	12	54	theme	GOS	1945:1947	arg1	compounds					1949:1957	GOS compounds	1945:1957	GOS compounds	1945:1957	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	8	55	theme	DP2	1184:1186	arg1	pool					1188:1191	the GOS DP2 pool	1176:1191	the GOS DP2 pool	1176:1191	All strains commonly used most of the GOS DP2 pool.					
31747272	12	56	theme	combinations	1891:1902	arg1	design					1871:1876	the design	1867:1876	the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth	1867:1998	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	6	57	theme	relative	944:951	arg1	OD600nm					936:942	OD600nm	936:942	OD600nm	936:942	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	6	57	theme	relative	944:951	arg1	%					931:931	30 and 100%	921:931	30 and 100% of OD600nm relative to positive controls with glucose	921:985	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	5	58	theme	gut	693:695	arg1	bacteria					697:704	gut bacteria	693:704	gut bacteria	693:704	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	12	59	theme	probiotic	1912:1920	arg1	strains					1932:1938	probiotic bacterial strains	1912:1938	probiotic bacterial strains with GOS compounds that specifically stimulate their growth	1912:1998	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	8	60	used	used	1163:1166	arg2	strains					1146:1152	All strains	1142:1152	All strains	1142:1152	All strains commonly used most of the GOS DP2 pool.					
31747272	4	61	theme	recent	480:485	arg1	years					487:491	recent years	480:491	recent years	480:491	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	2	62	used	used	279:282	arg2	mixtures					259:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	7	63	theme	pGOS	1025:1028	arg1	mixture					1030:1036	the pGOS mixture	1021:1036	the pGOS mixture that remain after growth	1021:1061	By identifying the components of the pGOS mixture that remain after growth, we showed that strains varied in their consumption of specific GOS compounds.					
31747272	11	64	theme	metabolic	1730:1738	arg1	activity					1740:1747	metabolic activity	1730:1747	metabolic activity	1730:1747	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	9	65	theme	Lactobacillus	1194:1206	arg1	W57					1219:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	1	66	theme	β-galactosidase	136:150	arg1	enzymes					152:158	Various β-galactosidase enzymes	128:158	Various β-galactosidase enzymes	128:158	Various β-galactosidase enzymes catalyze the trans-glycosylation reaction with lactose.					
31747272	7	67	theme	specific	1118:1125	arg1	compounds					1131:1139	specific GOS compounds	1118:1139	specific GOS compounds	1118:1139	By identifying the components of the pGOS mixture that remain after growth, we showed that strains varied in their consumption of specific GOS compounds.					
31747272	11	68	theme	GOS	1650:1652	arg1	profiles					1666:1673	these bacterial GOS consumption profiles	1634:1673	these bacterial GOS consumption profiles	1634:1673	We correlated these bacterial GOS consumption profiles with their genomic information and were able to relate metabolic activity with the presence of genome-encoded transporters and carbohydrate-active enzymes.					
31747272	12	69	with	strains	1932:1938	arg1	compounds					1949:1957	GOS compounds	1945:1957	GOS compounds	1945:1957	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	1	70	theme	trans-glycosylation	173:191	arg1	reaction					193:200	the trans-glycosylation reaction	169:200	the trans-glycosylation reaction with lactose	169:213	Various β-galactosidase enzymes catalyze the trans-glycosylation reaction with lactose.					
31747272	6	71	with	controls	965:972	arg1	glucose					979:985	glucose	979:985	glucose	979:985	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	0	72	theme	Probiotic	99:107	arg1	Strains					119:125	Probiotic Bacterial Strains	99:125	Probiotic Bacterial Strains	99:125	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	9	73	theme	DP3	1241:1243	arg1	-d-Glc					1298:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	10	74	theme	higher	1353:1358	arg1	DP					1360:1361	higher DP	1353:1361	higher DP	1353:1361	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	0	75	theme	Strains	119:125	arg1	Cultures					87:94	Single Cultures	80:94	Single Cultures of Probiotic Bacterial Strains	80:125	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	9	76	theme	compound	1254:1261	arg1	β-d-Galp-					1263:1271	compound β-d-Galp-	1254:1271	the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc	1237:1303	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	9	76	theme	compound	1254:1261	arg1	→					1275:1275	1 → 4)-[β-d-Galp-(1 → 2)	1273:1296	1 → 4)-[β-d-Galp-(1 → 2)	1273:1296	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	6	77	theme	positive	956:963	arg1	controls					965:972	positive controls	956:972	positive controls with glucose	956:985	Growth among the probiotic strains varied strongly between 30 and 100% of OD600nm relative to positive controls with glucose.					
31747272	3	78	theme	polymerization	402:415	arg1	degree					392:397	a degree	390:397	a degree of polymerization (DP) varying from 2-8	390:437	GOS consists mainly of compounds with a degree of polymerization (DP) varying from 2-8 and with diverse glycosidic linkages.					
31747272	5	79	theme	different	723:731	arg1	species					733:739	11 different species	720:739	11 different species	720:739	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	10	80	with	GOS	1344:1346	arg1	branching					1367:1375	branching	1367:1375	branching	1367:1375	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	10	80	with	GOS	1344:1346	arg1	DP					1360:1361	higher DP	1353:1361	higher DP	1353:1361	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	5	81	theme	Vivinal	780:786	arg1	sample					800:805	a Vivinal GOS-derived sample	778:805	a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS)	778:859	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	13	82	theme	synbiotic	2006:2014	arg1	combinations					2016:2027	Such synbiotic combinations	2001:2027	Such synbiotic combinations	2001:2027	Such synbiotic combinations may be of interest in food/feed and/or pharmacy/medicine applications.					
31747272	3	83	theme	glycosidic	456:465	arg1	linkages					467:474	diverse glycosidic linkages	448:474	diverse glycosidic linkages	448:474	GOS consists mainly of compounds with a degree of polymerization (DP) varying from 2-8 and with diverse glycosidic linkages.					
31747272	0	84	theme	Galactooligosaccharide	23:44	arg1	Molecules					46:54	Galactooligosaccharide Molecules	23:54	Galactooligosaccharide Molecules	23:54	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.					
31747272	9	85	used	utilized	1228:1235	arg2	W57					1219:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57	1194:1221	Lactobacillus salivarius W57 also utilized the DP3 branched compound β-d-Galp-(1 → 4)-[β-d-Galp-(1 → 2)]-d-Glc.					
31747272	4	86	theme	DP	586:587	arg1	terms					577:581	terms	577:581	terms of DP	577:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	10	87	theme	breve	1412:1416	arg1	20091					1422:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterium breve DSM 20091	1396:1426	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	2	88	theme	galactooligosaccharide	230:251	arg1	mixtures					259:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	5	89	theme	stationary	756:765	arg1	phase					767:771	stationary phase	756:771	stationary phase	756:771	In this work, 13 (single) probiotic strains of gut bacteria, belonging to 11 different species, were grown to stationary phase with a Vivinal GOS-derived sample purified to remove lactose and monosaccharides (pGOS).					
31747272	4	90	theme	commercial	550:559	arg1	mixtures					565:572	several commercial GOS mixtures	542:572	several commercial GOS mixtures in terms of DP	542:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	4	91	theme	mixtures	565:572	arg1	composition					527:537	the composition	523:537	the composition of several commercial GOS mixtures in terms of DP	523:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	10	92	theme	Lactobacillus	1429:1441	arg1	W37					1455:1457	Lactobacillus acidophilus W37	1429:1457	Lactobacillus acidophilus W37	1429:1457	Bifidobacterial strains tended to use GOS with higher DP and branching than lactobacilli; Bifidobacterium breve DSM 20091, Lactobacillus acidophilus W37, and Bifidobacterium infantis DSM 20088 were exceptional in using 38, 36, and 35 compounds, respectively, out of the 40 different structures identified in pGOS.					
31747272	4	93	dep	detail	516:521	arg1	composition					527:537	the composition	523:537	the composition of several commercial GOS mixtures in terms of DP	523:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	2	94	theme	GOS	254:256	arg1	mixtures					259:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures	216:266	The resulting galactooligosaccharide (GOS) mixtures are widely used in infant nutrition to stimulate growth of beneficial gut bacteria.					
31747272	4	95	theme	several	542:548	arg1	mixtures					565:572	several commercial GOS mixtures	542:572	several commercial GOS mixtures in terms of DP	542:587	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	4	96	theme	compounds	635:643	arg1	identity					608:615	the structural identity	593:615	the structural identity of the individual compounds	593:643	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	4	96	theme	compounds	635:643	arg1	detail					516:521	detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds	516:643	detail	516:521	In recent years, we have elucidated in detail the composition of several commercial GOS mixtures in terms of DP and the structural identity of the individual compounds.					
31747272	12	97	theme	synbiotic	1881:1889	arg1	combinations					1891:1902	synbiotic combinations	1881:1902	synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth	1881:1998	These detailed insights may support the design of synbiotic combinations pairing probiotic bacterial strains with GOS compounds that specifically stimulate their growth.					
31747272	7	98	theme	compounds	1131:1139	arg1	consumption					1103:1113	their consumption	1097:1113	their consumption of specific GOS compounds	1097:1139	By identifying the components of the pGOS mixture that remain after growth, we showed that strains varied in their consumption of specific GOS compounds.					
30565163	4	0	theme	α2,3-NeuAc	1358:1367	arg1	moiety					1369:1374	α2,3-NeuAc moiety	1358:1374	α2,3-NeuAc moiety	1358:1374	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	1	1	theme	spectrometry	269:280	arg1	areas					255:259	other areas	249:259	other areas of mass spectrometry	249:280	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	2	2	theme	electrospray	637:648	arg1	dissociation					668:679	electrospray collision-induced dissociation	637:679	electrospray collision-induced dissociation in ion trap	637:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	8	3	theme	Other	1704:1708	arg1	[M+X					1731:1734	[M+X	1731:1734	[M+X	1731:1734	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	8	3	theme	Other	1704:1708	arg1	ions					1725:1728	Other singly-charged ions	1704:1728	Other singly-charged ions ([M+X]+)	1704:1737	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	3	4	theme	sialylated	789:798	arg1	isomers					816:822	native sialylated oligosaccharide isomers	782:822	native sialylated oligosaccharide isomers	782:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	1	5	used	used	299:302	arg2	library					187:193	Reference spectral library	168:193	Reference spectral library searching	168:203	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	4	6	theme	terminal	1134:1141	arg1	monosaccharide					1143:1156	the terminal monosaccharide	1130:1156	the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides	1130:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	8	7	theme	cross-ring	1759:1768	arg1	cleavages					1770:1778	similar cross-ring cleavages	1751:1778	similar cross-ring cleavages	1751:1778	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	2	8	theme	ion	684:686	arg1	trap					688:691	ion trap	684:691	ion trap	684:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	9	theme	oligosaccharide	588:602	arg1	isomers					604:610	such sialylated oligosaccharide isomers	572:610	such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	572:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	10	theme	dramatic	1021:1028	arg1	effect					1030:1035	a dramatic effect	1019:1035	a dramatic effect	1019:1035	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	11	theme	isomers	816:822	arg1	isomers					816:822	native sialylated oligosaccharide isomers	782:822	native sialylated oligosaccharide isomers	782:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	11	theme	isomers	816:822	arg1	sets					774:777	five sets	769:777	five sets of native sialylated oligosaccharide isomers	769:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	12	theme	such	572:575	arg1	isomers					604:610	such sialylated oligosaccharide isomers	572:610	such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	572:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	13	theme	search	852:857	arg1	method					859:864	a spectral library search method	833:864	a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds	833:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	0	14	theme	Library	149:155	arg1	Searching					157:165	Spectrum Library Searching	140:165	Spectrum Library Searching	140:165	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	2	15	dep	dissociation	668:679	arg1	spectrometers					726:738	mass spectrometers	721:738	mass spectrometers	721:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	7	16	theme	observed	1671:1678	arg1	patterns					1694:1701	the observed fragmentation patterns	1667:1701	the observed fragmentation patterns	1667:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	4	17	theme	sialyl	1201:1206	arg1	pentasaccharides					1208:1223	sialyl pentasaccharides	1201:1223	sialyl pentasaccharides	1201:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	2	18	from	fragmentation	707:719	arg1	trap					688:691	ion trap	684:691	ion trap	684:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	4	19	from	moiety	1369:1374	arg1	abundant					1346:1353	abundant	1346:1353	abundant	1346:1353	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	3	20	theme	spectral	835:842	arg1	method					859:864	a spectral library search method	833:864	a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds	833:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	21	theme	precursor	919:927	arg1	[M+2X-H					933:939	the precursor ion [M+2X-H]	915:940	the precursor ion [M+2X-H]	915:940	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	7	22	theme	reasonable	1639:1648	arg1	explanations					1650:1661	reasonable explanations	1639:1661	reasonable explanations for the observed fragmentation patterns	1639:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	1	23	theme	Reference	168:176	arg1	library					187:193	Reference spectral library	168:193	Reference spectral library searching	168:203	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	2	24	theme	library	509:515	arg1	methods					524:530	library search methods	509:530	library search methods	509:530	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	0	25	from	Patterns	25:32	arg1	Spectra					53:59	the Tandem Mass Spectra	37:59	the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	37:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	0	26	theme	Sialylated	78:87	arg1	Oligosaccharides					89:104	Underivatized Sialylated Oligosaccharides	64:104	Underivatized Sialylated Oligosaccharides	64:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	5	27	dep	NeuAc-α2,3-Gal-β1,4-Y	1411:1431	arg1	Y=GlcNAc					1434:1441	Y=GlcNAc	1434:1441	Y=GlcNAc	1434:1441	The 2,4A3-H2O peak is specific to NeuAc-α2,3-Gal-β1,4-Y (Y=GlcNAc or Glc).					
30565163	5	27	dep	NeuAc-α2,3-Gal-β1,4-Y	1411:1431	arg1	Glc					1446:1448	Glc	1446:1448	Glc	1446:1448	The 2,4A3-H2O peak is specific to NeuAc-α2,3-Gal-β1,4-Y (Y=GlcNAc or Glc).					
30565163	7	28	theme	α2,3-NeuAc	1604:1613	arg1	structures					1615:1624	α2,6-NeuAc and α2,3-NeuAc structures	1589:1624	α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns	1589:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	4	29	from	abundant	1346:1353	arg1	moiety					1369:1374	α2,3-NeuAc moiety	1358:1374	α2,3-NeuAc moiety	1358:1374	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	2	30	theme	mass	721:724	arg1	spectrometers					726:738	mass spectrometers	721:738	mass spectrometers	721:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	31	theme	sialylated	1065:1074	arg1	compounds					1076:1084	the sialylated compounds	1061:1084	the sialylated compounds	1061:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	7	32	theme	α2,6-NeuAc	1589:1598	arg1	structures					1615:1624	α2,6-NeuAc and α2,3-NeuAc structures	1589:1624	α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns	1589:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	12	33	dep	Abstract	2073:2080	arg1	Graphical					2063:2071	Graphical	2063:2071	Graphical	2063:2071	Graphical Abstract.					
30565163	2	34	theme	beam-type	697:705	arg1	fragmentation					707:719	beam-type fragmentation	697:719	beam-type fragmentation	697:719	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	0	35	theme	Special	116:122	arg1	Suitability					124:134	Their Special Suitability	110:134	Their Special Suitability for Spectrum Library Searching	110:165	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	4	36	from	spectra	1257:1263	arg1	abundant					1235:1242	abundant	1235:1242	abundant	1235:1242	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	37	theme	2,4A3-H2O	1316:1324	arg1	fragment					1327:1334	(2,4A3-H2O) fragment	1315:1334	(2,4A3-H2O) fragment	1315:1334	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	2	38	theme	sialylated	377:386	arg1	oligosaccharides					388:403	sialylated oligosaccharides	377:403	sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	377:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	39	theme	acid	973:976	arg1	linkage					978:984	Sialic acid linkage	966:984	Sialic acid linkage	966:984	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	40	gly	sialylated	789:798	arg1	isomers					816:822	native sialylated oligosaccharide isomers	782:822	native sialylated oligosaccharide isomers	782:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	41	theme	tandem	462:467	arg1	MS/MS					488:492	MS/MS	488:492	MS/MS	488:492	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	41	theme	tandem	462:467	arg1	spectrometry					474:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry	411:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	411:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	7	42	theme	conformational	1554:1567	arg1	differences					1569:1579	major conformational differences	1548:1579	major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns	1548:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	4	43	from	abundant	1235:1242	arg1	species					1278:1284	species	1278:1284	species	1278:1284	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	43	from	abundant	1235:1242	arg1	spectra					1257:1263	the MS/MS spectra	1247:1263	the MS/MS spectra of [M+2X-H]	1247:1275	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	44	theme	glycans	1300:1306	arg1	spectra					1257:1263	the MS/MS spectra	1247:1263	the MS/MS spectra of [M+2X-H]	1247:1275	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	2	45	theme	pressure-matrix-assisted	423:446	arg1	MS/MS					488:492	MS/MS	488:492	MS/MS	488:492	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	45	theme	pressure-matrix-assisted	423:446	arg1	spectrometry					474:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry	411:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	411:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	9	46	theme	sialyl	1884:1889	arg1	isomers					1891:1897	sialyl isomers	1884:1897	sialyl isomers	1884:1897	Implemented in a searchable library, these spectral differences provide a facile method to distinguish sialyl isomers without derivatization.					
30565163	3	47	gly	sialylated	1065:1074	arg1	compounds					1076:1084	the sialylated compounds	1061:1084	the sialylated compounds	1061:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	9	48	theme	searchable	1798:1807	arg1	library					1809:1815	a searchable library	1796:1815	a searchable library	1796:1815	Implemented in a searchable library, these spectral differences provide a facile method to distinguish sialyl isomers without derivatization.					
30565163	0	49	theme	Mass	48:51	arg1	Spectra					53:59	the Tandem Mass Spectra	37:59	the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	37:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	7	50	theme	Theoretical	1516:1526	arg1	calculations					1528:1539	Theoretical calculations	1516:1539	Theoretical calculations	1516:1539	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	4	51	from	species	1278:1284	arg1	abundant					1235:1242	abundant	1235:1242	abundant	1235:1242	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	3	52	theme	MS/MS	752:756	arg1	spectra					758:764	MS/MS spectra	752:764	MS/MS spectra of five sets of native sialylated oligosaccharide isomers	752:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	8	53	theme	singly-charged	1710:1723	arg1	[M+X					1731:1734	[M+X	1731:1734	[M+X	1731:1734	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	8	53	theme	singly-charged	1710:1723	arg1	ions					1725:1728	Other singly-charged ions	1704:1728	Other singly-charged ions ([M+X]+)	1704:1737	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	4	54	theme	2,4A3	1101:1105	arg1	fragment					1118:1125	2,4A3 cross-ring fragment	1101:1125	2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides	1101:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	3	55	theme	sets	774:777	arg1	spectra					758:764	MS/MS spectra	752:764	MS/MS spectra of five sets of native sialylated oligosaccharide isomers	752:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	5	56	theme	2,4A3-H2O	1381:1389	arg1	specific					1399:1406	specific	1399:1406	specific	1399:1406	The 2,4A3-H2O peak is specific to NeuAc-α2,3-Gal-β1,4-Y (Y=GlcNAc or Glc).					
30565163	5	56	theme	2,4A3-H2O	1381:1389	arg1	peak					1391:1394	The 2,4A3-H2O peak	1377:1394	The 2,4A3-H2O peak	1377:1394	The 2,4A3-H2O peak is specific to NeuAc-α2,3-Gal-β1,4-Y (Y=GlcNAc or Glc).					
30565163	1	57	theme	mass	264:267	arg1	spectrometry					269:280	mass spectrometry	264:280	mass spectrometry	264:280	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	2	58	theme	collision-induced	650:666	arg1	dissociation					668:679	electrospray collision-induced dissociation	637:679	electrospray collision-induced dissociation in ion trap	637:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	59	from	study	332:336	arg1	analysis					365:372	analysis	365:372	analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	365:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	60	theme	oligosaccharide	800:814	arg1	isomers					816:822	native sialylated oligosaccharide isomers	782:822	native sialylated oligosaccharide isomers	782:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	61	attach	derived	624:630	arg1	dissociation					668:679	electrospray collision-induced dissociation	637:679	electrospray collision-induced dissociation in ion trap	637:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	61	attach	derived	624:630	arg2	MS/MS					618:622	MS/MS	618:622	MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	618:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	61	attach	derived	624:630	arg1	fragmentation					707:719	beam-type fragmentation	697:719	beam-type fragmentation	697:719	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	62	theme	native	782:787	arg1	isomers					816:822	native sialylated oligosaccharide isomers	782:822	native sialylated oligosaccharide isomers	782:822	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	4	63	theme	[M+2X-H	1268:1274	arg1	spectra					1257:1263	the MS/MS spectra	1247:1263	the MS/MS spectra of [M+2X-H]	1247:1275	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	8	64	theme	similar	1751:1757	arg1	cleavages					1770:1778	similar cross-ring cleavages	1751:1778	similar cross-ring cleavages	1751:1778	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	4	65	from	monosaccharide	1143:1156	arg1	pentasaccharides					1208:1223	sialyl pentasaccharides	1201:1223	sialyl pentasaccharides	1201:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	65	from	monosaccharide	1143:1156	arg1	fragment					1118:1125	2,4A3 cross-ring fragment	1101:1125	2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides	1101:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	65	from	monosaccharide	1143:1156	arg1	sialyllactosamines					1177:1194	sialyllactosamines	1177:1194	sialyllactosamines	1177:1194	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	4	65	from	monosaccharide	1143:1156	arg1	sialyllactoses					1161:1174	sialyllactoses	1161:1174	sialyllactoses	1161:1174	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	0	66	theme	Spectrum	140:147	arg1	Searching					157:165	Spectrum Library Searching	140:165	Spectrum Library Searching	140:165	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	7	67	theme	fragmentation	1680:1692	arg1	patterns					1694:1701	the observed fragmentation patterns	1667:1701	the observed fragmentation patterns	1667:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	2	68	theme	sialylated	577:586	arg1	isomers					604:610	such sialylated oligosaccharide isomers	572:610	such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	572:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	11	69	from	http	2021:2024	arg1	available					2008:2016	available	2008:2016	available	2008:2016	MS/MS spectra and tools are available at http://chemdata.nist.gov/glycan/spectra .					
30565163	1	70	from	compounds	236:244	arg1	areas					255:259	other areas	249:259	other areas of mass spectrometry	249:280	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	0	71	from	Suitability	124:134	arg1	Spectra					53:59	the Tandem Mass Spectra	37:59	the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	37:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	1	72	used	used	219:222	arg2	library					187:193	Reference spectral library	168:193	Reference spectral library searching	168:203	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	3	73	theme	library	844:850	arg1	method					859:864	a spectral library search method	833:864	a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds	833:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	74	theme	isomers	604:610	arg1	differentiation					553:567	the automated differentiation	539:567	the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	539:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	4	75	theme	cross-ring	1107:1116	arg1	fragment					1118:1125	2,4A3 cross-ring fragment	1101:1125	2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides	1101:1223	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	0	76	theme	Underivatized	64:76	arg1	Oligosaccharides					89:104	Underivatized Sialylated Oligosaccharides	64:104	Underivatized Sialylated Oligosaccharides	64:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	2	77	gly	sialylated	577:586	arg1	isomers					604:610	such sialylated oligosaccharide isomers	572:610	such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	572:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	3	78	theme	ion	929:931	arg1	[M+2X-H					933:939	the precursor ion [M+2X-H]	915:940	the precursor ion [M+2X-H]	915:940	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	79	theme	search	517:522	arg1	methods					524:530	library search methods	509:530	library search methods	509:530	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	1	80	dep	used	299:302	arg1	used					219:222	used	219:222	widely used to identify compounds in other areas of mass spectrometry	212:280	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	8	81	dep	ions	1725:1728	arg1	+					1736:1736	+	1736:1736	+	1736:1736	Other singly-charged ions ([M+X]+) do not show similar cross-ring cleavages.					
30565163	9	82	theme	spectral	1824:1831	arg1	differences					1833:1843	these spectral differences	1818:1843	these spectral differences	1818:1843	Implemented in a searchable library, these spectral differences provide a facile method to distinguish sialyl isomers without derivatization.					
30565163	0	83	theme	Oligosaccharides	89:104	arg1	Spectra					53:59	the Tandem Mass Spectra	37:59	the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	37:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	3	84	theme	compounds	1076:1084	arg1	fragmentation					1044:1056	the fragmentation	1040:1056	the fragmentation of the sialylated compounds	1040:1084	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	1	85	theme	spectral	178:185	arg1	library					187:193	Reference spectral library	168:193	Reference spectral library searching	168:203	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	10	86	theme	good	1937:1940	arg1	spectral					1942:1949	good spectral	1937:1949	good spectral matching across instruments	1937:1977	We also found good spectral matching across instruments.					
30565163	2	87	theme	automated	543:551	arg1	differentiation					553:567	the automated differentiation	539:567	the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers	539:738	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	88	theme	oligosaccharides	388:403	arg1	analysis					365:372	analysis	365:372	analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	365:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	0	89	theme	Fragmentation	11:23	arg1	Patterns					25:32	Fragmentation Patterns	11:32	Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	11:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	9	90	theme	facile	1855:1860	arg1	method					1862:1867	a facile method	1853:1867	a facile method to distinguish sialyl isomers without derivatization	1853:1920	Implemented in a searchable library, these spectral differences provide a facile method to distinguish sialyl isomers without derivatization.					
30565163	2	91	gly	sialylated	377:386	arg1	oligosaccharides					388:403	sialylated oligosaccharides	377:403	sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	377:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	4	92	theme	MS/MS	1251:1255	arg1	spectra					1257:1263	the MS/MS spectra	1247:1263	the MS/MS spectra of [M+2X-H]	1247:1275	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	3	93	dep	X=Li	950:953	arg1	α2,6					996:999	α2,6	996:999	α2,6	996:999	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	93	dep	X=Li	950:953	arg1	α2,3					987:990	α2,3	987:990	α2,3	987:990	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	93	dep	X=Li	950:953	arg1	linkage					978:984	Sialic acid linkage	966:984	Sialic acid linkage	966:984	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	94	theme	mass	469:472	arg1	MS/MS					488:492	MS/MS	488:492	MS/MS	488:492	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	94	theme	mass	469:472	arg1	spectrometry					474:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry	411:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	411:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	95	from	dissociation	668:679	arg1	trap					688:691	ion trap	684:691	ion trap	684:691	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	96	theme	laser-induced	448:460	arg1	MS/MS					488:492	MS/MS	488:492	MS/MS	488:492	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	96	theme	laser-induced	448:460	arg1	spectrometry					474:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry	411:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	411:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	0	97	theme	Tandem	41:46	arg1	Spectra					53:59	the Tandem Mass Spectra	37:59	the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides	37:104	Cross-Ring Fragmentation Patterns in the Tandem Mass Spectra of Underivatized Sialylated Oligosaccharides and Their Special Suitability for Spectrum Library Searching.					
30565163	3	98	theme	Sialic	966:971	arg1	linkage					978:984	Sialic acid linkage	966:984	Sialic acid linkage	966:984	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	99	contain	have	1014:1017	arg2	effect					1030:1035	a dramatic effect	1019:1035	a dramatic effect	1019:1035	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	99	contain	have	1014:1017	arg1	Na					956:957	Na	956:957	Na	956:957	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	99	contain	have	1014:1017	arg1	K.					963:964	K.	963:964	K.	963:964	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	3	99	contain	have	1014:1017	arg1	X=Li					950:953	X=Li	950:953	X=Li	950:953	We compare MS/MS spectra of five sets of native sialylated oligosaccharide isomers and show a spectral library search method that can distinguish between these isomers using the precursor ion [M+2X-H]+, where X=Li, Na, or K. Sialic acid linkage (α2,3 vs. α2,6) is known to have a dramatic effect on the fragmentation of the sialylated compounds.					
30565163	2	100	theme	atmospheric	411:421	arg1	MS/MS					488:492	MS/MS	488:492	MS/MS	488:492	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	2	100	theme	atmospheric	411:421	arg1	spectrometry					474:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry	411:485	atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS)	411:493	Building on a study by Cotter and coworkers on analysis of sialylated oligosaccharides using atmospheric pressure-matrix-assisted laser-induced tandem mass spectrometry (MS/MS), we show that library search methods enable the automated differentiation of such sialylated oligosaccharide isomers using MS/MS derived from electrospray collision-induced dissociation in ion trap and beam-type fragmentation mass spectrometers.					
30565163	7	101	theme	major	1548:1552	arg1	differences					1569:1579	major conformational differences	1548:1579	major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns	1548:1701	Theoretical calculations reveal major conformational differences between α2,6-NeuAc and α2,3-NeuAc structures that provide reasonable explanations for the observed fragmentation patterns.					
30565163	4	102	theme	α2,6-NeuAc	1289:1298	arg1	glycans					1300:1306	α2,6-NeuAc glycans	1289:1306	α2,6-NeuAc glycans	1289:1306	We found that 2,4A3 cross-ring fragment at the terminal monosaccharide in sialyllactoses, sialyllactosamines, and sialyl pentasaccharides is highly abundant in the MS/MS spectra of [M+2X-H]+ species of α2,6-NeuAc glycans, while (2,4A3-H2O) fragment is highly abundant in α2,3-NeuAc moiety.					
30565163	11	103	dep	http	2021:2024	arg1	//chemdata.nist.gov/glycan/spectra					2026:2059	//chemdata.nist.gov/glycan/spectra	2026:2059	http://chemdata.nist.gov/glycan/spectra	2021:2059	MS/MS spectra and tools are available at http://chemdata.nist.gov/glycan/spectra .					
30565163	1	104	theme	other	249:253	arg1	areas					255:259	other areas	249:259	other areas of mass spectrometry	249:280	Reference spectral library searching, while widely used to identify compounds in other areas of mass spectrometry, is not commonly used in glycomics.					
30565163	11	105	theme	MS/MS	1980:1984	arg1	spectra					1986:1992	MS/MS spectra	1980:1992	MS/MS spectra	1980:1992	MS/MS spectra and tools are available at http://chemdata.nist.gov/glycan/spectra .					
31200590	0	0	theme	Potential	67:75	arg1	Alterations					77:87	Potential Alterations	67:87	Potential Alterations	67:87	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.					
31200590	3	1	theme	coating	700:706	arg1	strategy					708:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	2	2	theme	non-mass	590:597	arg1	additives					626:634	non-mass spectrometry (MS) friendly additives	590:634	non-mass spectrometry (MS) friendly additives within the separation electrolyte	590:668	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	6	3	theme	alterations	1119:1129	arg1	set					1095:1097	The set	1091:1097	The set of potential glycan alterations	1091:1129	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	6	3	theme	alterations	1119:1129	arg1	alterations					1119:1129	potential glycan alterations	1102:1129	potential glycan alterations	1102:1129	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	2	4	theme	capillaries	472:482	arg1	use					450:452	the use	446:452	the use of neutral coated capillaries	446:482	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	1	5	attach	present	108:114	arg1	study					98:102	this study	93:102	this study	93:102	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	5	attach	present	108:114	arg2	we					105:106	we	105:106	we	105:106	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	6	6	theme	glycan	1112:1117	arg1	alterations					1119:1129	potential glycan alterations	1102:1129	potential glycan alterations	1102:1129	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	5	7	theme	disease	1013:1019	arg1	patients					1021:1028	Parkinson's disease patients	1001:1028	Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids	1001:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	3	8	theme	online	685:690	arg1	strategy					708:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	4	9	theme	disease	897:903	arg1	patients					905:912	15 Parkinson's disease patients	882:912	15 Parkinson's disease patients	882:912	The annotated structures were quantified in 15 controls and 15 Parkinson's disease patients by label-free quantitation.					
31200590	3	10	with	combination	778:788	arg1	sequencing					810:819	exoglycosidase sequencing	795:819	exoglycosidase sequencing	795:819	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	1	11	theme	vector	237:242	arg1	SVM-RFE					343:349	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	SVM-RFE	343:349	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	11	theme	vector	237:242	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	11	theme	vector	237:242	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	12	theme	coating	508:514	arg1	strategy					516:523	a dynamic capillary coating strategy	488:523	a dynamic capillary coating strategy	488:523	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	3	13	theme	developed	675:683	arg1	strategy					708:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	2	14	theme	additives	626:634	arg1	need					582:585	the need	578:585	the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte	578:668	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	1	15	theme	machine-based	244:256	arg1	SVM-RFE					343:349	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	SVM-RFE	343:349	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	15	theme	machine-based	244:256	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	15	theme	machine-based	244:256	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	16	from	alterations	381:391	arg1	disease					408:414	Parkinson's disease	396:414	Parkinson's disease	396:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	3	17	gly	N-glycosylation	747:761	arg1	combination					778:788	combination	778:788	combination with exoglycosidase sequencing	778:819	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	1	18	from	application	120:130	arg1	combination					184:194	combination	184:194	combination with label-free quantitation	184:223	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	6	19	theme	efficient	1206:1214	arg1	classification					1216:1229	the efficient classification	1202:1229	the efficient classification of male patients	1202:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	1	20	theme	novel	137:141	arg1	method					174:179	a novel capillary electrophoresis (CE) method	135:179	a novel capillary electrophoresis (CE) method	135:179	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	21	theme	dynamic	490:496	arg1	strategy					516:523	a dynamic capillary coating strategy	488:523	a dynamic capillary coating strategy	488:523	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	1	22	theme	potential	364:372	arg1	alterations					381:391	potential glycan alterations	364:391	potential glycan alterations in Parkinson's disease	364:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	23	theme	separation	647:656	arg1	electrolyte					658:668	the separation electrolyte	643:668	the separation electrolyte	643:668	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	0	24	theme	Serum	0:4	arg1	N-Glycosylation					6:20	Serum N-Glycosylation	0:20	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.	0:88	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.					
31200590	5	25	from	patients	1021:1028	arg1	glycans					1047:1053	tri-antennary glycans	1033:1053	tri-antennary glycans with 2 and 3 terminal sialic acids	1033:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	1	26	theme	capillary	143:151	arg1	electrophoresis					153:167	capillary electrophoresis	143:167	a novel capillary electrophoresis (CE) method	135:179	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	26	theme	capillary	143:151	arg1	CE					170:171	CE	170:171	CE	170:171	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	5	27	theme	2	1060:1060	arg1	acids					1084:1088	2 and 3 terminal sialic acids	1060:1088	2 and 3 terminal sialic acids	1060:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	1	28	theme	glycan	374:379	arg1	alterations					381:391	potential glycan alterations	364:391	potential glycan alterations in Parkinson's disease	364:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	3	29	from	N-glycosylation	747:761	arg1	combination					778:788	combination	778:788	combination with exoglycosidase sequencing	778:819	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	5	30	theme	increased	964:972	arg1	fucosylation					974:985	increased fucosylation	964:985	increased fucosylation	964:985	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	1	31	theme	electrophoresis	153:167	arg1	method					174:179	a novel capillary electrophoresis (CE) method	135:179	a novel capillary electrophoresis (CE) method	135:179	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	0	32	from	N-Glycosylation	6:20	arg1	Disease					37:43	Parkinson's Disease	25:43	Parkinson's Disease	25:43	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.					
31200590	1	33	theme	feature	258:264	arg1	SVM-RFE					343:349	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	SVM-RFE	343:349	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	33	theme	feature	258:264	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	33	theme	feature	258:264	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	34	theme	spectrometry	599:610	arg1	additives					626:634	non-mass spectrometry (MS) friendly additives	590:634	non-mass spectrometry (MS) friendly additives within the separation electrolyte	590:668	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	6	35	theme	potential	1102:1110	arg1	alterations					1119:1129	potential glycan alterations	1102:1129	potential glycan alterations	1102:1129	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	5	36	theme	tri-antennary	1033:1045	arg1	glycans					1047:1053	tri-antennary glycans	1033:1053	tri-antennary glycans with 2 and 3 terminal sialic acids	1033:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	2	37	theme	stable	536:541	arg1	separations					558:568	stable and repeatable separations	536:568	stable and repeatable separations	536:568	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	2	38	theme	friendly	617:624	arg1	additives					626:634	non-mass spectrometry (MS) friendly additives	590:634	non-mass spectrometry (MS) friendly additives within the separation electrolyte	590:668	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	6	39	theme	elimination	1167:1177	arg1	algorithm					1179:1187	a recursive feature elimination algorithm	1147:1187	a recursive feature elimination algorithm resulting in the efficient classification of male patients	1147:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	6	40	theme	patients	1239:1246	arg1	classification					1216:1229	the efficient classification	1202:1229	the efficient classification of male patients	1202:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	2	41	theme	Specific	417:424	arg1	focus					426:430	Specific focus	417:430	Specific focus	417:430	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	2	42	theme	coated	465:470	arg1	capillaries					472:482	neutral coated capillaries	457:482	neutral coated capillaries	457:482	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	1	43	theme	support	277:283	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	43	theme	support	277:283	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	44	theme	method	174:179	arg1	application					120:130	the application	116:130	the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation	116:223	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	45	theme	neutral	457:463	arg1	capillaries					472:482	neutral coated capillaries	457:482	neutral coated capillaries	457:482	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	1	46	theme	vector	285:290	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	46	theme	vector	285:290	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	3	47	theme	exoglycosidase	795:808	arg1	sequencing					810:819	exoglycosidase sequencing	795:819	exoglycosidase sequencing	795:819	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	5	48	dep	Lower	942:946	arg1	sialylation					948:958	sialylation	948:958	sialylation	948:958	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	5	48	dep	Lower	942:946	arg1	fucosylation					974:985	increased fucosylation	964:985	increased fucosylation	964:985	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	5	49	located	found	992:996	arg1	patients					1021:1028	Parkinson's disease patients	1001:1028	Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids	1001:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	5	49	located	found	992:996	arg2	Lower					942:946	Lower	942:946	Lower	942:946	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	1	50	theme	machine-estimated	292:308	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	50	theme	machine-estimated	292:308	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	5	51	with	glycans	1047:1053	arg1	acids					1084:1088	2 and 3 terminal sialic acids	1060:1088	2 and 3 terminal sialic acids	1060:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	3	52	theme	serum	741:745	arg1	N-glycosylation					747:761	serum N-glycosylation	741:761	serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing	741:819	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	6	53	theme	feature	1159:1165	arg1	algorithm					1179:1187	a recursive feature elimination algorithm	1147:1187	a recursive feature elimination algorithm resulting in the efficient classification of male patients	1147:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	1	54	theme	recursive	310:318	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	54	theme	recursive	310:318	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	5	55	theme	terminal	1068:1075	arg1	acids					1084:1088	2 and 3 terminal sialic acids	1060:1088	2 and 3 terminal sialic acids	1060:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	5	56	theme	3	1066:1066	arg1	acids					1084:1088	2 and 3 terminal sialic acids	1060:1088	2 and 3 terminal sialic acids	1060:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	6	57	theme	recursive	1149:1157	arg1	algorithm					1179:1187	a recursive feature elimination algorithm	1147:1187	a recursive feature elimination algorithm resulting in the efficient classification of male patients	1147:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	1	58	theme	feature	320:326	arg1	elimination					328:338	support vector machine-estimated recursive feature elimination or SVM-RFE	277:349	elimination	328:338	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	1	58	theme	feature	320:326	arg1	selection					266:274	vector machine-based feature selection	237:274	vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease	237:414	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	2	59	theme	repeatable	547:556	arg1	separations					558:568	stable and repeatable separations	536:568	stable and repeatable separations	536:568	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	5	60	theme	sialic	1077:1082	arg1	acids					1084:1088	2 and 3 terminal sialic acids	1060:1088	2 and 3 terminal sialic acids	1060:1088	Lower sialylation and increased fucosylation were found in Parkinson's disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids.					
31200590	4	61	theme	annotated	826:834	arg1	structures					836:845	The annotated structures	822:845	The annotated structures	822:845	The annotated structures were quantified in 15 controls and 15 Parkinson's disease patients by label-free quantitation.					
31200590	4	62	theme	label-free	917:926	arg1	quantitation					928:939	label-free quantitation	917:939	label-free quantitation	917:939	The annotated structures were quantified in 15 controls and 15 Parkinson's disease patients by label-free quantitation.					
31200590	2	63	theme	capillary	498:506	arg1	strategy					516:523	a dynamic capillary coating strategy	488:523	a dynamic capillary coating strategy	488:523	Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte.					
31200590	0	64	dep	N-Glycosylation	6:20	arg1	Approach					54:61	A Novel Approach	46:61	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.	0:88	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.					
31200590	1	65	theme	label-free	201:210	arg1	quantitation					212:223	label-free quantitation	201:223	label-free quantitation	201:223	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
31200590	0	66	theme	Novel	48:52	arg1	Approach					54:61	A Novel Approach	46:61	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.	0:88	Serum N-Glycosylation in Parkinson's Disease: A Novel Approach for Potential Alterations.					
31200590	3	67	theme	dynamic	692:698	arg1	strategy					708:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy	671:715	The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing.					
31200590	6	68	theme	male	1234:1237	arg1	patients					1239:1246	male patients	1234:1246	male patients	1234:1246	The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.					
31200590	1	69	with	combination	184:194	arg1	quantitation					212:223	label-free quantitation	201:223	label-free quantitation	201:223	In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson's disease.					
30268928	3	0	from	model	417:421	arg1	pathogenic					375:384	pathogenic	375:384	pathogenic	375:384	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	3	1	theme	GXM-like	518:525	arg1	molecules					527:535	GXM-like molecules	518:535	GXM-like molecules	518:535	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	5	2	theme	T.	764:765	arg1	glycan					776:781	the T. mucoides glycan	760:781	the T. mucoides glycan	760:781	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	4	3	theme	Compositional	538:550	arg1	analysis					552:559	Compositional analysis	538:559	Compositional analysis of samples obtained from both pathogens	538:599	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	7	4	theme	yeast	1111:1115	arg1	forms					1117:1121	T. mucoides yeast forms	1099:1121	T. mucoides yeast forms	1099:1121	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	3	5	theme	molecules	527:535	arg1	characteristics					499:513	the characteristics	495:513	the characteristics of GXM-like molecules	495:535	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	8	6	theme	mucoides	1213:1220	arg1	glycan					1222:1227	the T. mucoides glycan	1206:1227	the T. mucoides glycan	1206:1227	Experiments with animal cells revealed that the T. mucoides glycan manifested antiphagocytic properties.					
30268928	7	7	theme	T.	1099:1100	arg1	forms					1117:1121	T. mucoides yeast forms	1099:1121	T. mucoides yeast forms	1099:1121	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	7	8	theme	cryptococcal	1071:1082	arg1	GXM					1084:1086	cryptococcal GXM	1071:1086	cryptococcal GXM	1071:1086	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	5	9	theme	GXM-like	788:795	arg1	molecule					797:804	a GXM-like molecule	786:804	a GXM-like molecule	786:804	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	5	10	theme	acapsular	952:960	arg1	mutant					962:967	an acapsular mutant	949:967	an acapsular mutant of C. neoformans	949:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	8	11	theme	antiphagocytic	1240:1253	arg1	properties					1255:1264	antiphagocytic properties	1240:1264	antiphagocytic properties	1240:1264	Experiments with animal cells revealed that the T. mucoides glycan manifested antiphagocytic properties.					
30268928	1	12	theme	immunomodulatory	175:190	arg1	polysaccharide					199:212	an immunomodulatory fungal polysaccharide	172:212	an immunomodulatory fungal polysaccharide	172:212	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	1	12	theme	immunomodulatory	175:190	arg1	glucuronoxylomannan					145:163	glucuronoxylomannan	145:163	glucuronoxylomannan (GXM)	145:169	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	4	13	located	detected	691:698	arg1	mucoides					706:713	T. mucoides	703:713	T. mucoides	703:713	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	4	13	located	detected	691:698	arg1	asahii					722:727	T. asahii	719:727	T. asahii	719:727	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	4	13	located	detected	691:698	arg1	fact					685:688	fact	685:688	fact	685:688	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	4	13	located	detected	691:698	arg2	components					620:629	the components	616:629	the components of GXM (mannose, xylose and glucuronic acid)	616:674	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	10	14	theme	GXM	1485:1487	arg1	receptors					1459:1467	the typical receptors	1447:1467	the typical receptors of cryptococcal GXM	1447:1487	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	5	15	theme	mutant	962:967	arg1	surface					938:944	the cell surface	929:944	the cell surface of an acapsular mutant of C. neoformans	929:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	1	16	theme	fungal	192:197	arg1	polysaccharide					199:212	an immunomodulatory fungal polysaccharide	172:212	an immunomodulatory fungal polysaccharide	172:212	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	1	16	theme	fungal	192:197	arg1	glucuronoxylomannan					145:163	glucuronoxylomannan	145:163	glucuronoxylomannan (GXM)	145:169	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	0	17	theme	glucuronoxylomannan-like	2:25	arg1	glycan					27:32	A glucuronoxylomannan-like glycan	0:32	A glucuronoxylomannan-like glycan	0:32	A glucuronoxylomannan-like glycan produced by Trichosporon mucoides.					
30268928	10	18	theme	Tlr2-/-	1547:1553	arg1	mice					1576:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	10	19	theme	cryptococcal	1472:1483	arg1	GXM					1485:1487	cryptococcal GXM	1472:1487	cryptococcal GXM	1472:1487	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	10	20	with	association	1397:1407	arg1	phagocytes					1418:1427	the phagocytes	1414:1427	the phagocytes	1414:1427	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	1	21	theme	Cryptococcus	101:112	arg1	species					114:120	Cryptococcus species	101:120	Cryptococcus species	101:120	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	3	22	theme	mellonella	435:444	arg1	model					417:421	an infection model	404:421	an infection model of Galleria mellonella	404:444	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	7	23	dep	T.	1099:1100	arg1	mucoides					1102:1109	mucoides	1102:1109	mucoides	1102:1109	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	9	24	theme	phagocytosis	1279:1290	arg1	assays					1292:1297	Comparative phagocytosis assays	1267:1297	Comparative phagocytosis assays	1267:1297	Comparative phagocytosis assays revealed that T. mucoides and T. asahii were similarly recognized by macrophages.					
30268928	11	25	theme	major	1726:1730	arg1	immunogen					1739:1747	this major fungal immunogen	1721:1747	this major fungal immunogen	1721:1747	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	11	26	theme	pathogens	1634:1642	arg1	list					1619:1622	the list	1615:1622	the list of fungal pathogens producing GXM-like glycans	1615:1669	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	6	27	theme	molecular	1033:1041	arg1	dimensions					1043:1052	molecular dimensions	1033:1052	molecular dimensions	1033:1052	T. mucoides and T. asahii glycans differed in molecular dimensions.					
30268928	7	28	theme	T.	1145:1146	arg1	cells					1155:1159	T. asahii cells	1145:1159	T. asahii cells	1145:1159	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	5	29	with	reactivity	827:836	arg1	antibody					856:863	a monoclonal antibody	843:863	a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans	843:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	10	30	theme	fungal	1390:1395	arg1	association					1397:1407	fungal association	1390:1407	fungal association with the phagocytes	1390:1427	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	4	31	dep	mucoides	706:713	arg1	glycans					729:735	glycans	729:735	glycans	729:735	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	7	32	theme	asahii	1148:1153	arg1	cells					1155:1159	T. asahii cells	1145:1159	T. asahii cells	1145:1159	The antibody to cryptococcal GXM recognized T. mucoides yeast forms less efficiently than T. asahii cells.					
30268928	5	33	dep	T.	764:765	arg1	mucoides					767:774	mucoides	767:774	mucoides	767:774	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	11	34	theme	functional	1697:1706	arg1	diversity					1708:1716	a high functional diversity	1690:1716	a high functional diversity of this major fungal immunogen	1690:1747	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	2	35	theme	Trichosporon	261:272	arg1	species					250:256	other opportunistic species	230:256	other opportunistic species of Trichosporon	230:272	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	3	36	theme	Galleria	426:433	arg1	mellonella					435:444	Galleria mellonella	426:444	Galleria mellonella	426:444	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	5	37	theme	glycan	776:781	arg1	identification					742:755	The identification	738:755	The identification of the T. mucoides glycan as a GXM-like molecule	738:804	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	3	38	from	pathogenic	375:384	arg1	model					417:421	an infection model	404:421	an infection model of Galleria mellonella	404:444	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	11	39	theme	immunogen	1739:1747	arg1	diversity					1708:1716	a high functional diversity	1690:1716	a high functional diversity of this major fungal immunogen	1690:1747	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	4	40	theme	glucuronic	659:668	arg1	acid					670:673	glucuronic acid	659:673	glucuronic acid	659:673	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	3	41	theme	infection	407:415	arg1	model					417:421	an infection model	404:421	an infection model of Galleria mellonella	404:444	In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this difference was related to the characteristics of GXM-like molecules.					
30268928	6	42	theme	asahii	1006:1011	arg1	glycans					1013:1019	T. mucoides and T. asahii glycans	987:1019	T. mucoides and T. asahii glycans	987:1019	T. mucoides and T. asahii glycans differed in molecular dimensions.					
30268928	10	43	theme	Cd14-/-	1559:1565	arg1	mice					1576:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	2	44	theme	species	250:256	arg1	ability					219:225	The ability	215:225	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides	215:308	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	2	44	theme	species	250:256	arg1	unknown					313:319	unknown	313:319	unknown	313:319	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	10	45	theme	typical	1451:1457	arg1	receptors					1459:1467	the typical receptors	1447:1467	the typical receptors of cryptococcal GXM	1447:1487	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	2	46	theme	other	230:234	arg1	species					250:256	other opportunistic species	230:256	other opportunistic species of Trichosporon	230:272	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	2	47	theme	opportunistic	236:248	arg1	species					250:256	other opportunistic species	230:256	other opportunistic species of Trichosporon	230:272	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	4	48	theme	samples	564:570	arg1	analysis					552:559	Compositional analysis	538:559	Compositional analysis of samples obtained from both pathogens	538:599	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	11	49	theme	fungal	1732:1737	arg1	immunogen					1739:1747	this major fungal immunogen	1721:1747	this major fungal immunogen	1721:1747	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	8	50	with	Experiments	1162:1172	arg1	cells					1186:1190	animal cells	1179:1190	animal cells	1179:1190	Experiments with animal cells revealed that the T. mucoides glycan manifested antiphagocytic properties.					
30268928	1	51	dep	Trichosporon	69:80	arg1	asahii					82:87	Trichosporon asahii	69:87	Trichosporon asahii	69:87	Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide.					
30268928	5	52	theme	cell	933:936	arg1	surface					938:944	the cell surface	929:944	the cell surface of an acapsular mutant of C. neoformans	929:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	9	53	theme	Comparative	1267:1277	arg1	assays					1292:1297	Comparative phagocytosis assays	1267:1297	Comparative phagocytosis assays	1267:1297	Comparative phagocytosis assays revealed that T. mucoides and T. asahii were similarly recognized by macrophages.					
30268928	2	54	theme	GXM-like	285:292	arg1	polysaccharides					294:308	GXM-like polysaccharides	285:308	GXM-like polysaccharides	285:308	The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown.					
30268928	5	55	theme	glycan	917:922	arg1	GXM					888:890	cryptococcal GXM	875:890	cryptococcal GXM	875:890	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	5	55	theme	glycan	917:922	arg1	incorporation					896:908	incorporation	896:908	incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans	896:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	4	56	theme	GXM	634:636	arg1	components					620:629	the components	616:629	the components of GXM (mannose, xylose and glucuronic acid)	616:674	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	10	57	theme	knockout	1567:1574	arg1	mice					1576:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	Tlr2-/- and Cd14-/- knockout mice	1547:1579	However, fungal association with the phagocytes did not depend on the typical receptors of cryptococcal GXM, as concluded from assays using macrophages obtained from Tlr2-/- and Cd14-/- knockout mice.					
30268928	4	58	dep	GXM	634:636	arg1	xylose					648:653	xylose	648:653	xylose	648:653	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	4	58	dep	GXM	634:636	arg1	acid					670:673	glucuronic acid	659:673	glucuronic acid	659:673	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	4	58	dep	GXM	634:636	arg1	mannose					639:645	mannose	639:645	mannose	639:645	Compositional analysis of samples obtained from both pathogens indicated that the components of GXM (mannose, xylose and glucuronic acid) were, in fact, detected in T. mucoides and T. asahii glycans.					
30268928	5	59	theme	neoformans	975:984	arg1	mutant					962:967	an acapsular mutant	949:967	an acapsular mutant of C. neoformans	949:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	11	60	theme	fungal	1627:1632	arg1	pathogens					1634:1642	fungal pathogens	1627:1642	fungal pathogens producing GXM-like glycans	1627:1669	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	5	61	theme	monoclonal	845:854	arg1	antibody					856:863	a monoclonal antibody	843:863	a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans	843:984	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	6	62	theme	mucoides	990:997	arg1	glycans					1013:1019	T. mucoides and T. asahii glycans	987:1019	T. mucoides and T. asahii glycans	987:1019	T. mucoides and T. asahii glycans differed in molecular dimensions.					
30268928	8	63	theme	T.	1210:1211	arg1	glycan					1222:1227	the T. mucoides glycan	1206:1227	the T. mucoides glycan	1206:1227	Experiments with animal cells revealed that the T. mucoides glycan manifested antiphagocytic properties.					
30268928	11	64	theme	GXM-like	1654:1661	arg1	glycans					1663:1669	GXM-like glycans	1654:1669	GXM-like glycans	1654:1669	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
30268928	8	65	theme	animal	1179:1184	arg1	cells					1186:1190	animal cells	1179:1190	animal cells	1179:1190	Experiments with animal cells revealed that the T. mucoides glycan manifested antiphagocytic properties.					
30268928	5	66	theme	cryptococcal	875:886	arg1	GXM					888:890	cryptococcal GXM	875:890	cryptococcal GXM	875:890	The identification of the T. mucoides glycan as a GXM-like molecule was confirmed by its reactivity with a monoclonal antibody raised to cryptococcal GXM and incorporation of the glycan into the cell surface of an acapsular mutant of C. neoformans.					
30268928	11	67	theme	high	1692:1695	arg1	diversity					1708:1716	a high functional diversity	1690:1716	a high functional diversity of this major fungal immunogen	1690:1747	These results add T. mucoides to the list of fungal pathogens producing GXM-like glycans, but also indicate a high functional diversity of this major fungal immunogen.					
31726038	3	0	theme	functional	496:505	arg1	foods					507:511	functional foods	496:511	functional foods	496:511	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	7	1	theme	shallow	1149:1155	arg1	site					1164:1167	a shallow active site	1147:1167	a shallow active site that is housed in the (β/α)8 domain	1147:1203	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	2	2	theme	natural	272:278	arg1	products					280:287	these natural products	266:287	these natural products	266:287	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	2	3	theme	natural	136:142	arg1	products					144:151	Flavonoid natural products	126:151	Flavonoid natural products	126:151	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	6	4	theme	RHA-P	901:905	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	4	theme	RHA-P	901:905	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	7	5	theme	β/α	1192:1194	arg1	domain					1198:1203	the (β/α)8 domain	1187:1203	the (β/α)8 domain	1187:1203	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	10	6	theme	organic	1953:1959	arg1	solvents					1961:1968	organic solvents	1953:1968	organic solvents	1953:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	7	7	theme	calcium-binding	1119:1133	arg1	residues					1135:1142	calcium-binding residues	1119:1142	calcium-binding residues	1119:1142	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	3	8	theme	libraries	565:573	arg1	modification					533:544	the modification	529:544	the modification of natural product libraries being tested for drug discovery	529:605	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	3	9	theme	natural	549:555	arg1	libraries					565:573	natural product libraries	549:573	natural product libraries being tested for drug discovery	549:605	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	10	10	from	efficiency	1904:1913	arg1	concentrations					1935:1948	significant concentrations	1923:1948	significant concentrations of organic solvents	1923:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	6	11	theme	RHA-P	970:974	arg1	characterization					950:965	the characterization	946:965	the characterization of RHA-P	946:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	8	12	theme	known	1254:1258	arg1	structure					1260:1268	the only known structure	1245:1268	the only known structure of an RHA-P homolog	1245:1288	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	12	theme	known	1254:1258	arg1	different					1409:1417	different	1409:1417	different	1409:1417	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	2	13	theme	anti-inflammatory	219:235	arg1	properties					237:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	8	14	gly	glycosylated	1480:1491	arg1	rutin					1538:1542	rutin	1538:1542	rutin	1538:1542	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	14	gly	glycosylated	1480:1491	arg1	quercitrin					1549:1558	quercitrin	1549:1558	quercitrin	1549:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	14	gly	glycosylated	1480:1491	arg1	naringin					1528:1535	naringin	1528:1535	naringin	1528:1535	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	14	gly	glycosylated	1480:1491	arg1	compounds					1498:1506	glycosylated aryl compounds	1480:1506	glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin	1480:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	14	gly	glycosylated	1480:1491	arg1	hesperidin					1516:1525	hesperidin	1516:1525	hesperidin	1516:1525	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	15	theme	substrate	1446:1454	arg1	preference					1456:1465	the substrate preference	1442:1465	the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin	1442:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	16	theme	RHA-P	1276:1280	arg1	homolog					1282:1288	an RHA-P homolog	1273:1288	an RHA-P homolog	1273:1288	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	6	17	theme	rhamnosylated	1035:1047	arg1	compounds					1057:1065	rhamnosylated natural compounds	1035:1065	rhamnosylated natural compounds	1035:1065	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	4	18	theme	bacterium	690:698	arg1	sp					716:717	the marine bacterium Novosphingobium sp	679:717	the marine bacterium Novosphingobium sp	679:717	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	9	19	theme	rhamnosylated	1776:1788	arg1	flavonoids					1790:1799	different rhamnosylated flavonoids	1766:1799	different rhamnosylated flavonoids	1766:1799	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	9	20	theme	kinetic	1733:1739	arg1	data					1741:1744	the known kinetic data	1723:1744	the known kinetic data for RHA-P acting on different rhamnosylated flavonoids	1723:1799	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	8	21	theme	aryl	1493:1496	arg1	rutin					1538:1542	rutin	1538:1542	rutin	1538:1542	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	21	theme	aryl	1493:1496	arg1	quercitrin					1549:1558	quercitrin	1549:1558	quercitrin	1549:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	21	theme	aryl	1493:1496	arg1	naringin					1528:1535	naringin	1528:1535	naringin	1528:1535	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	21	theme	aryl	1493:1496	arg1	compounds					1498:1506	glycosylated aryl compounds	1480:1506	glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin	1480:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	21	theme	aryl	1493:1496	arg1	hesperidin					1516:1525	hesperidin	1516:1525	hesperidin	1516:1525	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	22	theme	electrostatic	1307:1319	arg1	potentials					1321:1330	electrostatic potentials	1307:1330	electrostatic potentials	1307:1330	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	5	23	theme	α-1,6	753:757	arg1	linkages					780:787	α-1,6 and α-1,2 glycosidic linkages	753:787	α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety	753:838	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	5	24	theme	α-1,2	763:767	arg1	linkages					780:787	α-1,6 and α-1,2 glycosidic linkages	753:787	α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety	753:838	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	3	25	theme	rhamnosidases	382:394	arg1	lack					363:366	The lack	359:366	The lack of endogenous rhamnosidases in the human GI tract	359:416	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	8	26	theme	acid	1342:1345	arg1	composition					1347:1357	amino acid composition	1336:1357	amino acid composition	1336:1357	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	9	27	theme	different	1766:1774	arg1	flavonoids					1790:1799	different rhamnosylated flavonoids	1766:1799	different rhamnosylated flavonoids	1766:1799	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	3	28	theme	glycosylated	450:461	arg1	compounds					463:471	these glycosylated compounds	444:471	these glycosylated compounds	444:471	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	8	29	dep	pdbID	1231:1235	arg1	5MQN					1238:1241	5MQN	1238:1241	5MQN	1238:1241	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	3	30	theme	GI	409:410	arg1	tract					412:416	the human GI tract	399:416	the human GI tract	399:416	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	8	31	theme	substrate	1366:1374	arg1	pocket					1384:1389	the substrate binding pocket	1362:1389	the substrate binding pocket	1362:1389	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	9	32	dep	in	1663:1664	arg1	silico					1666:1671	silico	1666:1671	silico	1666:1671	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	6	33	theme	crystal	880:886	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	33	theme	crystal	880:886	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	4	34	dep	efficient	633:641	arg1	thermostable					644:655	thermostable	644:655	thermostable	644:655	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	6	35	theme	compounds	1057:1065	arg1	bioavailability					1016:1030	the bioavailability	1012:1030	the bioavailability of rhamnosylated natural compounds	1012:1065	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	8	36	theme	pocket	1384:1389	arg1	potentials					1321:1330	electrostatic potentials	1307:1330	electrostatic potentials	1307:1330	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	36	theme	pocket	1384:1389	arg1	different					1409:1417	different	1409:1417	different	1409:1417	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	36	theme	pocket	1384:1389	arg1	structure					1260:1268	the only known structure	1245:1268	the only known structure of an RHA-P homolog	1245:1288	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	36	theme	pocket	1384:1389	arg1	composition					1347:1357	amino acid composition	1336:1357	amino acid composition	1336:1357	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	36	theme	pocket	1384:1389	arg1	morphology					1295:1304	the morphology	1291:1304	the morphology	1291:1304	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	5	37	theme	a	821:821	arg1	moiety					833:838	a terminal rhamnose and a flavonoid moiety	797:838	moiety	833:838	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	6	38	theme	2.2 Å	863:867	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	38	theme	2.2 Å	863:867	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	4	39	theme	marine	683:688	arg1	sp					716:717	the marine bacterium Novosphingobium sp	679:717	the marine bacterium Novosphingobium sp	679:717	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	2	40	theme	beneficial	178:187	arg1	properties					237:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	6	41	theme	forward	935:941	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	41	theme	forward	935:941	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	0	42	from	sp	116:117	arg1	RHA-P					89:93	RHA-P	89:93	RHA-P from Novosphingobium sp	89:117	The crystal structure and insight into the substrate specificity of the α-L rhamnosidase RHA-P from Novosphingobium sp.					
31726038	6	43	from	forward	935:941	arg1	characterization					950:965	the characterization	946:965	the characterization of RHA-P	946:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	7	44	theme	active	1157:1162	arg1	site					1164:1167	a shallow active site	1147:1167	a shallow active site that is housed in the (β/α)8 domain	1147:1203	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	10	45	theme	catalytic	1894:1902	arg1	efficiency					1904:1913	catalytic efficiency	1894:1913	catalytic efficiency even in significant concentrations of organic solvents	1894:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	6	46	theme	essential	920:928	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	46	theme	essential	920:928	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	10	47	theme	known	1869:1873	arg1	rhamnosidases					1875:1887	other known rhamnosidases	1863:1887	other known rhamnosidases	1863:1887	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	10	48	from	thermostability	1835:1849	arg1	concentrations					1935:1948	significant concentrations	1923:1948	significant concentrations of organic solvents	1923:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	7	49	from	rhamnose-	1105:1113	arg1	site					1164:1167	a shallow active site	1147:1167	a shallow active site that is housed in the (β/α)8 domain	1147:1203	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	2	50	theme	Flavonoid	126:134	arg1	products					144:151	Flavonoid natural products	126:151	Flavonoid natural products	126:151	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	3	51	theme	product	557:563	arg1	libraries					565:573	natural product libraries	549:573	natural product libraries being tested for drug discovery	549:605	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	6	52	theme	potential	981:989	arg1	catalyst					991:998	a potential catalyst	979:998	a potential catalyst to increase the bioavailability of rhamnosylated natural compounds	979:1065	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	9	53	theme	in	1663:1664	arg1	docking					1673:1679	in silico docking	1663:1679	in silico docking	1663:1679	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	2	54	theme	antitumor	204:212	arg1	properties					237:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	7	55	theme	conserved	1095:1103	arg1	rhamnose-					1105:1113	highly conserved rhamnose-	1088:1113	highly conserved rhamnose-	1088:1113	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	3	56	from	value	487:491	arg1	foods					507:511	functional foods	496:511	functional foods	496:511	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	9	57	theme	docking	1673:1679	arg1	consistent					1707:1716	consistent	1707:1716	consistent	1707:1716	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	9	57	theme	docking	1673:1679	arg1	results					1686:1692	the results	1682:1692	the results	1682:1692	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	10	58	theme	significant	1923:1933	arg1	concentrations					1935:1948	significant concentrations	1923:1948	significant concentrations of organic solvents	1923:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	4	59	theme	efficient	633:641	arg1	α-l-rhamnosidase					657:672	a catalytically efficient, thermostable α-l-rhamnosidase	617:672	a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp	617:717	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	4	59	theme	efficient	633:641	arg1	RHA-P					608:612	RHA-P	608:612	RHA-P	608:612	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	3	60	from	lack	363:366	arg1	tract					412:416	the human GI tract	399:416	the human GI tract	399:416	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	10	61	theme	solvents	1961:1968	arg1	concentrations					1935:1948	significant concentrations	1923:1948	significant concentrations of organic solvents	1923:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	6	62	theme	natural	1049:1055	arg1	compounds					1057:1065	rhamnosylated natural compounds	1035:1065	rhamnosylated natural compounds	1035:1065	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	8	63	theme	RHA-P	1470:1474	arg1	preference					1456:1465	the substrate preference	1442:1465	the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin	1442:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	5	64	theme	glycosidic	769:778	arg1	linkages					780:787	α-1,6 and α-1,2 glycosidic linkages	753:787	α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety	753:838	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	9	65	with	consistent	1707:1716	arg1	data					1741:1744	the known kinetic data	1723:1744	the known kinetic data for RHA-P acting on different rhamnosylated flavonoids	1723:1799	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	8	66	theme	glycosylated	1480:1491	arg1	rutin					1538:1542	rutin	1538:1542	rutin	1538:1542	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	66	theme	glycosylated	1480:1491	arg1	quercitrin					1549:1558	quercitrin	1549:1558	quercitrin	1549:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	66	theme	glycosylated	1480:1491	arg1	naringin					1528:1535	naringin	1528:1535	naringin	1528:1535	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	66	theme	glycosylated	1480:1491	arg1	compounds					1498:1506	glycosylated aryl compounds	1480:1506	glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin	1480:1558	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	66	theme	glycosylated	1480:1491	arg1	hesperidin					1516:1525	hesperidin	1516:1525	hesperidin	1516:1525	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	67	theme	homolog	1282:1288	arg1	potentials					1321:1330	electrostatic potentials	1307:1330	electrostatic potentials	1307:1330	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	67	theme	homolog	1282:1288	arg1	different					1409:1417	different	1409:1417	different	1409:1417	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	67	theme	homolog	1282:1288	arg1	structure					1260:1268	the only known structure	1245:1268	the only known structure of an RHA-P homolog	1245:1288	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	67	theme	homolog	1282:1288	arg1	composition					1347:1357	amino acid composition	1336:1357	amino acid composition	1336:1357	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	8	67	theme	homolog	1282:1288	arg1	morphology					1295:1304	the morphology	1291:1304	the morphology	1291:1304	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	0	68	theme	α-L	72:74	arg1	specificity					53:63	the substrate specificity	39:63	the substrate specificity of the α-L	39:74	The crystal structure and insight into the substrate specificity of the α-L rhamnosidase RHA-P from Novosphingobium sp.					
31726038	9	69	theme	known	1727:1731	arg1	data					1741:1744	the known kinetic data	1723:1744	the known kinetic data for RHA-P acting on different rhamnosylated flavonoids	1723:1799	These preferences were further explored by using in silico docking, the results of which are consistent with the known kinetic data for RHA-P acting on different rhamnosylated flavonoids.					
31726038	4	70	theme	Novosphingobium	700:714	arg1	sp					716:717	the marine bacterium Novosphingobium sp	679:717	the marine bacterium Novosphingobium sp	679:717	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	10	71	theme	biocatalytic	2009:2020	arg1	applications					2022:2033	biocatalytic applications	2009:2033	biocatalytic applications	2009:2033	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	0	72	theme	Novosphingobium	100:114	arg1	sp					116:117	Novosphingobium sp	100:117	Novosphingobium sp	100:117	The crystal structure and insight into the substrate specificity of the α-L rhamnosidase RHA-P from Novosphingobium sp.					
31726038	5	73	theme	flavonoid	823:831	arg1	moiety					833:838	a terminal rhamnose and a flavonoid moiety	797:838	moiety	833:838	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	8	74	theme	amino	1336:1340	arg1	composition					1347:1357	amino acid composition	1336:1357	amino acid composition	1336:1357	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	3	75	theme	endogenous	371:380	arg1	rhamnosidases					382:394	endogenous rhamnosidases	371:394	endogenous rhamnosidases	371:394	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	7	76	from	residues	1135:1142	arg1	site					1164:1167	a shallow active site	1147:1167	a shallow active site that is housed in the (β/α)8 domain	1147:1203	The structure shows highly conserved rhamnose- and calcium-binding residues in a shallow active site that is housed in the (β/α)8 domain.					
31726038	8	77	dep	BT0986	1223:1228	arg1	comparison					1209:1218	comparison	1209:1218	comparison	1209:1218	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	10	78	theme	relative	1826:1833	arg1	thermostability					1835:1849	relative thermostability	1826:1849	relative thermostability compared to other known rhamnosidases	1826:1887	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	3	79	theme	compounds	463:471	arg1	many					436:439	many	436:439	many	436:439	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	3	79	theme	compounds	463:471	arg1	compounds					463:471	these glycosylated compounds	444:471	these glycosylated compounds	444:471	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	5	80	theme	terminal	799:806	arg1	rhamnose					808:815	a terminal rhamnose and a flavonoid moiety	797:838	rhamnose	808:815	PP1Y that selectively hydrolyzes α-1,6 and α-1,2 glycosidic linkages between a terminal rhamnose and a flavonoid moiety.					
31726038	3	81	gly	glycosylated	450:461	arg1	compounds					463:471	these glycosylated compounds	444:471	these glycosylated compounds	444:471	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	0	82	theme	substrate	43:51	arg1	specificity					53:63	the substrate specificity	39:63	the substrate specificity of the α-L	39:74	The crystal structure and insight into the substrate specificity of the α-L rhamnosidase RHA-P from Novosphingobium sp.					
31726038	6	83	theme	resolution	869:878	arg1	step					930:933	an essential step	917:933	an essential step forward in the characterization of RHA-P	917:974	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	6	83	theme	resolution	869:878	arg1	structure					888:896	the 2.2 Å resolution crystal structure	859:896	the 2.2 Å resolution crystal structure	859:896	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	3	84	theme	human	403:407	arg1	tract					412:416	the human GI tract	399:416	the human GI tract	399:416	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	10	85	theme	other	1863:1867	arg1	rhamnosidases					1875:1887	other known rhamnosidases	1863:1887	other known rhamnosidases	1863:1887	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	8	86	theme	binding	1376:1382	arg1	pocket					1384:1389	the substrate binding pocket	1362:1389	the substrate binding pocket	1362:1389	In comparison to BT0986 (pdbID: 5MQN), the only known structure of an RHA-P homolog, the morphology, electrostatic potentials and amino acid composition of the substrate binding pocket are significantly different, offering insight into the substrate preference of RHA-P for glycosylated aryl compounds such as hesperidin, naringin, rutin, and quercitrin, over polysaccharides, which are preferred by BT0986.					
31726038	3	87	theme	drug	592:595	arg1	discovery					597:605	drug discovery	592:605	drug discovery	592:605	The lack of endogenous rhamnosidases in the human GI tract not only prevents many of these glycosylated compounds from being of value in functional foods but also limits the modification of natural product libraries being tested for drug discovery.					
31726038	6	88	from	characterization	950:965	arg1	forward					935:941	forward	935:941	forward	935:941	This work reports the 2.2 Å resolution crystal structure of RHA-P, which is an essential step forward in the characterization of RHA-P as a potential catalyst to increase the bioavailability of rhamnosylated natural compounds.					
31726038	2	89	theme	antimicrobial	189:201	arg1	properties					237:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	their beneficial antimicrobial, antitumor, and anti-inflammatory properties	172:246	Flavonoid natural products are well known for their beneficial antimicrobial, antitumor, and anti-inflammatory properties, however, some of these natural products often are rhamnosylated, which severely limits their bioavailability.					
31726038	10	90	from	promiscuity	1813:1823	arg1	concentrations					1935:1948	significant concentrations	1923:1948	significant concentrations of organic solvents	1923:1968	Due to its promiscuity, relative thermostability compared to other known rhamnosidases, and catalytic efficiency even in significant concentrations of organic solvents, RHA-P continues to show potential for biocatalytic applications.					
31726038	0	91	theme	crystal	4:10	arg1	structure					12:20	The crystal structure	0:20	The crystal structure	0:20	The crystal structure and insight into the substrate specificity of the α-L rhamnosidase RHA-P from Novosphingobium sp.					
31726038	4	92	from	sp	716:717	arg1	α-l-rhamnosidase					657:672	a catalytically efficient, thermostable α-l-rhamnosidase	617:672	a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp	617:717	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
31726038	4	92	from	sp	716:717	arg1	RHA-P					608:612	RHA-P	608:612	RHA-P	608:612	RHA-P is a catalytically efficient, thermostable α-l-rhamnosidase from the marine bacterium Novosphingobium sp.					
29343515	5	0	theme	bioinformatics	740:753	arg1	approaches					786:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	10	1	theme	Gram-positive	1811:1823	arg1	bacteria					1825:1832	Gram-positive bacteria	1811:1832	Gram-positive bacteria	1811:1832	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	4	2	theme	LTA	678:680	arg1	glycosylation					661:673	the glycosylation	657:673	the glycosylation of LTA	657:680	However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized.					
29343515	0	3	theme	distinct	77:84	arg1	mechanisms					86:95	two distinct mechanisms	73:95	two distinct mechanisms for wall teichoic acid glycosylation	73:132	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	9	4	theme	similar	1570:1576	arg1	function					1561:1568	a function	1559:1568	a function similar to that of YfhO	1559:1592	Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous.					
29343515	3	5	theme	many	483:486	arg1	bacteria					502:509	many Gram-positive bacteria	483:509	many Gram-positive bacteria	483:509	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	6	6	theme	YfhO	963:966	arg1	lmo1079					938:944	the Listeria monocytogenes gene lmo1079	906:944	the Listeria monocytogenes gene lmo1079	906:944	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	6	6	theme	YfhO	963:966	arg1	homolog					968:974	a YfhO homolog	961:974	a YfhO homolog	961:974	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	0	7	theme	wall	101:104	arg1	acid					115:118	wall teichoic acid	101:118	wall teichoic acid glycosylation	101:132	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	8	8	theme	WTA	1347:1349	arg1	glycosylation					1351:1363	WTA glycosylation	1347:1363	WTA glycosylation	1347:1363	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	4	9	theme	proteins	632:639	arg1	function					616:623	the function	612:623	the function of and proteins responsible for the glycosylation of LTA	612:680	However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized.					
29343515	4	9	theme	proteins	632:639	arg1	unknown					693:699	unknown	693:699	unknown	693:699	However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized.					
29343515	10	10	theme	updated	1683:1689	arg1	model					1691:1695	an updated model	1680:1695	an updated model for LTA glycosylation	1680:1717	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	7	11	gly	glycosylated	1134:1145	arg1	LTA					1066:1068	LTA	1066:1068	LTA	1066:1068	LTA is polymerized on the outside of the cell and hence can only be glycosylated extracellularly.					
29343515	6	12	theme	WTA	1047:1049	arg1	glycosylation					1051:1063	WTA glycosylation	1047:1063	WTA glycosylation	1047:1063	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	2	13	theme	Gram-positive	325:337	arg1	wall					354:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	0	14	theme	acid	115:118	arg1	glycosylation					120:132	wall teichoic acid glycosylation	101:132	wall teichoic acid glycosylation	101:132	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	9	15	contain	has	1555:1557	arg1	protein					1547:1553	the encoded protein	1535:1553	the encoded protein	1535:1553	Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous.					
29343515	9	15	contain	has	1555:1557	arg2	function					1561:1568	a function	1559:1568	a function similar to that of YfhO	1559:1592	Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous.					
29343515	1	16	theme	important	165:173	arg1	wall					154:157	The bacterial cell wall	135:157	The bacterial cell wall	135:157	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	16	theme	important	165:173	arg1	structure					194:202	an important and highly complex structure	162:202	an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults	162:312	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	16	theme	important	165:173	arg1	essential					212:220	essential	212:220	essential	212:220	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	0	17	theme	teichoic	106:113	arg1	acid					115:118	wall teichoic acid	101:118	wall teichoic acid glycosylation	101:132	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	5	18	theme	genetic	756:762	arg1	approaches					786:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	10	19	theme	WTA	1759:1761	arg1	glycosylation					1742:1754	glycosylation	1742:1754	glycosylation of WTA	1742:1761	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	2	20	theme	secondary	396:404	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	20	theme	secondary	396:404	arg1	acid					440:443	wall teichoic acid	426:443	wall teichoic acid (WTA)	426:449	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	20	theme	secondary	396:404	arg1	acid					468:471	lipoteichoic acid	455:471	lipoteichoic acid (LTA)	455:477	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	5	21	theme	spectroscopy	773:784	arg1	approaches					786:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	bioinformatics, genetic, and NMR spectroscopy approaches	740:795	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	6	22	theme	gene	933:936	arg1	lmo1079					938:944	the Listeria monocytogenes gene lmo1079	906:944	the Listeria monocytogenes gene lmo1079	906:944	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	6	22	theme	gene	933:936	arg1	homolog					968:974	a YfhO homolog	961:974	a YfhO homolog	961:974	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	6	22	theme	gene	933:936	arg1	essential					1033:1041	essential	1033:1041	essential	1033:1041	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	2	23	theme	typical	317:323	arg1	wall					354:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	8	24	theme	genes	1195:1199	arg1	similarity					1177:1186	the similarity	1173:1186	the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation	1173:1324	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	10	25	from	mechanisms	1797:1806	arg1	bacteria					1825:1832	Gram-positive bacteria	1811:1832	Gram-positive bacteria	1811:1832	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	1	26	theme	cell	277:280	arg1	lysis					282:286	cell lysis	277:286	cell lysis	277:286	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	9	27	theme	LTA	1500:1502	arg1	glycosylation					1504:1516	LTA glycosylation	1500:1516	LTA glycosylation	1500:1516	Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous.					
29343515	1	28	theme	complex	186:192	arg1	wall					154:157	The bacterial cell wall	135:157	The bacterial cell wall	135:157	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	28	theme	complex	186:192	arg1	structure					194:202	an important and highly complex structure	162:202	an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults	162:312	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	28	theme	complex	186:192	arg1	essential					212:220	essential	212:220	essential	212:220	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	3	29	theme	polyglycerol-phosphate	521:542	arg1	chain					544:548	a polyglycerol-phosphate chain	519:548	a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues	519:600	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	3	29	theme	polyglycerol-phosphate	521:542	arg1	LTA					512:514	LTA	512:514	LTA	512:514	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	2	30	theme	wall	426:429	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	30	theme	wall	426:429	arg1	WTA					446:448	WTA	446:448	WTA	446:448	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	30	theme	wall	426:429	arg1	acid					440:443	wall teichoic acid	426:443	wall teichoic acid (WTA)	426:449	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	31	theme	teichoic	431:438	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	31	theme	teichoic	431:438	arg1	WTA					446:448	WTA	446:448	WTA	446:448	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	31	theme	teichoic	431:438	arg1	acid					440:443	wall teichoic acid	426:443	wall teichoic acid (WTA)	426:449	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	8	32	theme	WTA	1308:1310	arg1	glycosylation					1312:1324	WTA glycosylation	1308:1324	WTA glycosylation	1308:1324	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	3	33	theme	Gram-positive	488:500	arg1	bacteria					502:509	many Gram-positive bacteria	483:509	many Gram-positive bacteria	483:509	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	0	34	theme	lipoteichoic	32:43	arg1	glycosylation					50:62	lipoteichoic acid glycosylation	32:62	lipoteichoic acid glycosylation	32:62	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	1	35	theme	environmental	292:304	arg1	insults					306:312	environmental insults	292:312	environmental insults	292:312	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	10	36	gly	glycosylation	1742:1754	arg1	WTA					1759:1761	WTA	1759:1761	WTA	1759:1761	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	4	37	theme	responsible	641:651	arg1	proteins					632:639	and proteins	628:639	proteins	632:639	However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized.					
29343515	6	38	theme	LTA	998:1000	arg1	glycosylation					1002:1014	LTA glycosylation	998:1014	LTA glycosylation	998:1014	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	2	39	theme	lipoteichoic	455:466	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	39	theme	lipoteichoic	455:466	arg1	LTA					474:476	LTA	474:476	LTA	474:476	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	39	theme	lipoteichoic	455:466	arg1	acid					468:471	lipoteichoic acid	455:471	lipoteichoic acid (LTA)	455:477	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	6	40	theme	monocytogenes	919:931	arg1	lmo1079					938:944	the Listeria monocytogenes gene lmo1079	906:944	the Listeria monocytogenes gene lmo1079	906:944	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	6	40	theme	monocytogenes	919:931	arg1	homolog					968:974	a YfhO homolog	961:974	a YfhO homolog	961:974	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	6	40	theme	monocytogenes	919:931	arg1	essential					1033:1041	essential	1033:1041	essential	1033:1041	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	8	41	gly	glycosylation	1351:1363	arg2	species					1410:1416	Listeria species	1401:1416	Listeria species	1401:1416	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	8	42	theme	Listeria	1401:1408	arg1	species					1410:1416	Listeria species	1401:1416	Listeria species	1401:1416	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	10	43	theme	LTA	1701:1703	arg1	glycosylation					1705:1717	LTA glycosylation	1701:1717	LTA glycosylation	1701:1717	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	2	44	theme	wall	411:414	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	44	theme	wall	411:414	arg1	acid					440:443	wall teichoic acid	426:443	wall teichoic acid (WTA)	426:449	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	44	theme	wall	411:414	arg1	acid					468:471	lipoteichoic acid	455:471	lipoteichoic acid (LTA)	455:477	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	6	45	theme	Listeria	910:917	arg1	monocytogenes					919:931	Listeria monocytogenes	910:931	the Listeria monocytogenes gene lmo1079	906:944	Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation.					
29343515	5	46	theme	NMR	769:771	arg1	spectroscopy					773:784	NMR spectroscopy	769:784	NMR spectroscopy	769:784	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	5	47	theme	LTA	872:874	arg1	glycosylation					876:888	LTA glycosylation	872:888	LTA glycosylation	872:888	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	0	48	theme	acid	45:48	arg1	glycosylation					50:62	lipoteichoic acid glycosylation	32:62	lipoteichoic acid glycosylation	32:62	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
29343515	2	49	theme	cell	406:409	arg1	polymers					416:423	the secondary cell wall polymers	392:423	the secondary cell wall polymers	392:423	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	49	theme	cell	406:409	arg1	acid					440:443	wall teichoic acid	426:443	wall teichoic acid (WTA)	426:449	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	2	49	theme	cell	406:409	arg1	acid					468:471	lipoteichoic acid	455:471	lipoteichoic acid (LTA)	455:477	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	4	50	gly	glycosylation	661:673	arg1	LTA					678:680	LTA	678:680	LTA	678:680	However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized.					
29343515	10	51	theme	different	1787:1795	arg1	mechanisms					1797:1806	two different mechanisms	1783:1806	two different mechanisms in Gram-positive bacteria	1783:1832	Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.					
29343515	2	52	theme	cell	349:352	arg1	wall					354:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	5	53	theme	yfhO	843:846	arg1	essential					858:866	essential	858:866	essential	858:866	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	5	53	theme	yfhO	843:846	arg1	genes					848:852	the Bacillus subtilis csbB and yfhO genes	812:852	the Bacillus subtilis csbB and yfhO genes	812:852	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	9	54	theme	encoded	1539:1545	arg1	protein					1547:1553	the encoded protein	1535:1553	the encoded protein	1535:1553	Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous.					
29343515	2	55	theme	bacterial	339:347	arg1	wall					354:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall	315:357	A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA).					
29343515	3	56	from	chain	544:548	arg1	bacteria					502:509	many Gram-positive bacteria	483:509	many Gram-positive bacteria	483:509	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	8	57	theme	YfhO	1212:1215	arg1	homologs					1217:1224	YfhO homologs	1212:1224	YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation	1212:1324	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	5	58	dep	Bacillus	816:823	arg1	subtilis					825:832	subtilis	825:832	subtilis	825:832	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	1	59	theme	bacterial	139:147	arg1	wall					154:157	The bacterial cell wall	135:157	The bacterial cell wall	135:157	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	59	theme	bacterial	139:147	arg1	structure					194:202	an important and highly complex structure	162:202	an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults	162:312	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	59	theme	bacterial	139:147	arg1	essential					212:220	essential	212:220	essential	212:220	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	8	60	theme	LTA	1263:1265	arg1	glycosylation					1267:1279	LTA glycosylation	1263:1279	LTA glycosylation	1263:1279	Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species.					
29343515	1	61	theme	bacterial	226:234	arg1	growth					236:241	bacterial growth	226:241	bacterial growth	226:241	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	5	62	theme	Bacillus	816:823	arg1	csbB					834:837	Bacillus subtilis csbB	816:837	Bacillus subtilis csbB	816:837	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	3	63	theme	sugar	587:591	arg1	residues					593:600	sugar residues	587:600	sugar residues	587:600	In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues.					
29343515	1	64	theme	cell	149:152	arg1	wall					154:157	The bacterial cell wall	135:157	The bacterial cell wall	135:157	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	64	theme	cell	149:152	arg1	structure					194:202	an important and highly complex structure	162:202	an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults	162:312	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	1	64	theme	cell	149:152	arg1	essential					212:220	essential	212:220	essential	212:220	The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults.					
29343515	5	65	theme	csbB	834:837	arg1	essential					858:866	essential	858:866	essential	858:866	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	5	65	theme	csbB	834:837	arg1	genes					848:852	the Bacillus subtilis csbB and yfhO genes	812:852	the Bacillus subtilis csbB and yfhO genes	812:852	Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation.					
29343515	0	66	theme	genes	13:17	arg1	Discovery					0:8	Discovery	0:8	Discovery of genes required for lipoteichoic acid glycosylation	0:62	Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.					
31549691	2	0	contain	containing	436:445	arg1	disaccharides					422:434	disaccharides	422:434	disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal	422:528	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	2	0	contain	containing	436:445	arg2	moiety					468:473	the 2-deoxygalactose moiety	447:473	the 2-deoxygalactose moiety	447:473	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	1	1	theme	nucleophiles	227:238	arg1	addition					207:214	the direct stereoselective addition	180:214	the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal	180:278	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	3	2	theme	large	766:770	arg1	excess					772:777	a large excess	764:777	a large excess of nucleophiles or additives	764:806	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	3	theme	functional	674:683	arg1	groups					685:690	functional groups	674:690	functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives	674:806	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	4	4	theme	α-2-deoxygalactosides	876:896	arg1	variety					865:871	a variety	863:871	a variety of α-2-deoxygalactosides	863:896	The method is potentially useful for the synthesis of a variety of α-2-deoxygalactosides.					
31549691	4	4	theme	α-2-deoxygalactosides	876:896	arg1	α-2-deoxygalactosides					876:896	α-2-deoxygalactosides	876:896	α-2-deoxygalactosides	876:896	The method is potentially useful for the synthesis of a variety of α-2-deoxygalactosides.					
31549691	3	5	theme	groups	685:690	arg1	range					665:669	a range	663:669	a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives	663:806	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	5	theme	groups	685:690	arg1	acceptors					639:647	acceptors	639:647	acceptors	639:647	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	6	theme	alcohol	615:621	arg1	nucleophiles					623:634	alcohol nucleophiles	615:634	alcohol nucleophiles	615:634	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	7	theme	nucleophiles	623:634	arg1	range					606:610	a wide range	599:610	a wide range of alcohol nucleophiles	599:634	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	8	theme	glycosylation	562:574	arg1	method					576:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	1	9	theme	deactivated	243:253	arg1	d-galactal					269:278	deactivated peracetylated d-galactal	243:278	deactivated peracetylated d-galactal	243:278	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	3	10	theme	convenient	536:545	arg1	method					576:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	1	11	theme	peracetylated	255:267	arg1	d-galactal					269:278	deactivated peracetylated d-galactal	243:278	deactivated peracetylated d-galactal	243:278	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	0	12	theme	acid-catalyzed	23:36	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	0	13	from	synthesis	63:71	arg1	deactivated					101:111	deactivated	101:111	deactivated	101:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	1	14	theme	Perfluorophenylboronic	139:160	arg1	1c					167:168	Perfluorophenylboronic acid 1c	139:168	Perfluorophenylboronic acid 1c	139:168	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	2	15	theme	peracetylated	505:517	arg1	d-galactal					519:528	the deactivated peracetylated d-galactal	489:528	the deactivated peracetylated d-galactal	489:528	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	0	16	theme	Perfluorophenylboronic	0:21	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	1	17	theme	acid	162:165	arg1	1c					167:168	Perfluorophenylboronic acid 1c	139:168	Perfluorophenylboronic acid 1c	139:168	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	2	18	theme	deactivated	493:503	arg1	d-galactal					519:528	the deactivated peracetylated d-galactal	489:528	the deactivated peracetylated d-galactal	489:528	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	0	19	theme	α-stereoselective	45:61	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	3	20	theme	wide	601:604	arg1	range					606:610	a wide range	599:610	a wide range of alcohol nucleophiles	599:634	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	0	21	theme	direct	38:43	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	4	22	theme	variety	865:871	arg1	synthesis					850:858	the synthesis	846:858	the synthesis of a variety of α-2-deoxygalactosides	846:896	The method is potentially useful for the synthesis of a variety of α-2-deoxygalactosides.					
31549691	3	23	theme	byproduct	729:737	arg1	formation					704:712	the formation	700:712	the formation of the Ferrier byproduct	700:737	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	24	theme	metal-free	551:560	arg1	method					576:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method	531:581	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	1	25	with	2-deoxygalactosides	288:306	arg1	α-selectivity					338:350	complete α-selectivity	329:350	complete α-selectivity	329:350	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	1	26	theme	55-88	311:315	arg1	%					316:316	%	316:316	%	316:316	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	0	27	from	deactivated	101:111	arg1	2-deoxygalactosides					76:94	2-deoxygalactosides	76:94	2-deoxygalactosides from deactivated	76:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	0	27	from	deactivated	101:111	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	2	28	theme	2-deoxygalactose	451:466	arg1	moiety					468:473	the 2-deoxygalactose moiety	447:473	the 2-deoxygalactose moiety	447:473	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	0	29	theme	2-deoxygalactosides	76:94	arg1	synthesis					63:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis	0:71	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated	0:111	Perfluorophenylboronic acid-catalyzed direct α-stereoselective synthesis of 2-deoxygalactosides from deactivated peracetylated d-galactal.					
31549691	3	30	theme	additives	798:806	arg1	excess					772:777	a large excess	764:777	a large excess of nucleophiles or additives	764:806	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	3	31	theme	Ferrier	721:727	arg1	byproduct					729:737	the Ferrier byproduct	717:737	the Ferrier byproduct	717:737	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	1	32	theme	direct	184:189	arg1	addition					207:214	the direct stereoselective addition	180:214	the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal	180:278	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	2	33	theme	unprecedented	357:369	arg1	results					371:377	The unprecedented results	353:377	The unprecedented results reported here	353:391	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	1	34	from	2-deoxygalactosides	288:306	arg1	yield					318:322	55-88% yield	311:322	55-88% yield	311:322	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	1	35	theme	stereoselective	191:205	arg1	addition					207:214	the direct stereoselective addition	180:214	the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal	180:278	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	1	36	theme	%	316:316	arg1	yield					318:322	55-88% yield	311:322	55-88% yield	311:322	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	3	37	theme	nucleophiles	782:793	arg1	excess					772:777	a large excess	764:777	a large excess of nucleophiles or additives	764:806	This convenient and metal-free glycosylation method works well with a wide range of alcohol nucleophiles as acceptors and tolerates a range of functional groups without the formation of the Ferrier byproduct and without the need for a large excess of nucleophiles or additives.					
31549691	2	38	theme	disaccharides	422:434	arg1	synthesis					409:417	the synthesis	405:417	the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal	405:528	The unprecedented results reported here also enable the synthesis of disaccharides containing the 2-deoxygalactose moiety directly from the deactivated peracetylated d-galactal.					
31549691	1	39	theme	alcohol	219:225	arg1	nucleophiles					227:238	alcohol nucleophiles	219:238	alcohol nucleophiles	219:238	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
31549691	1	40	theme	complete	329:336	arg1	α-selectivity					338:350	complete α-selectivity	329:350	complete α-selectivity	329:350	Perfluorophenylboronic acid 1c catalyzes the direct stereoselective addition of alcohol nucleophiles to deactivated peracetylated d-galactal to give 2-deoxygalactosides in 55-88% yield with complete α-selectivity.					
29628253	0	0	theme	DSM	96:98	arg1	20193					100:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	6	1	theme	1b	898:899	arg1	dextran					901:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	0	2	theme	pseudomesenteroides	76:94	arg1	20193					100:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	1	3	theme	study	151:155	arg1	aim					139:141	The aim	135:141	The aim of this study	135:155	The aim of this study was to study the interactions between dextran and fava bean protein.					
29628253	4	4	theme	dextran	678:684	arg1	conjugation					663:673	the conjugation	659:673	the conjugation of dextran to FPI	659:691	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	8	5	theme	mixed	1093:1097	arg1	systems					1099:1105	the mixed systems	1089:1105	the mixed systems	1089:1105	The intermolecular FPI-dextran interactions played an important role in stabilizing the mixed systems at different pH.					
29628253	5	6	from	influences	799:808	arg1	properties					854:863	rheological properties	842:863	rheological properties	842:863	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	2	7	theme	DSM	283:285	arg1	20193					287:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	5	8	theme	rheological	842:852	arg1	properties					854:863	rheological properties	842:863	rheological properties	842:863	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	0	9	theme	cibaria	120:126	arg1	1b					131:132	Weissella cibaria Sj 1b	110:132	Weissella cibaria Sj 1b	110:132	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	4	10	theme	FPI	623:625	arg1	incubation					609:618	Dry-heating incubation	597:618	Dry-heating incubation of FPI and dextran mixture	597:645	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	2	11	theme	pseudomesenteroides	263:281	arg1	20193					287:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	0	12	theme	Weissella	110:118	arg1	1b					131:132	Weissella cibaria Sj 1b	110:132	Weissella cibaria Sj 1b	110:132	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	5	13	theme	formed	817:822	arg1	bonds					833:837	the formed covalent bonds	813:837	the formed covalent bonds	813:837	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	2	14	theme	Leuconostoc	251:261	arg1	20193					287:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Leuconostoc pseudomesenteroides DSM 20193	251:291	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	8	15	theme	different	1110:1118	arg1	pH					1120:1121	different pH	1110:1121	different pH	1110:1121	The intermolecular FPI-dextran interactions played an important role in stabilizing the mixed systems at different pH.					
29628253	7	16	dep	dextran	996:1002	arg1	pseudomesenteroides					966:984	pseudomesenteroides	966:984	pseudomesenteroides	966:984	pseudomesenteroides DSM 20193 dextran.					
29628253	2	17	theme	Sj	315:316	arg1	1b					318:319	Weissella cibaria Sj 1b	297:319	Weissella cibaria Sj 1b	297:319	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	6	18	theme	gel-strengthening	927:943	arg1	ability					945:951	a much higher gel-strengthening ability	913:951	a much higher gel-strengthening ability	913:951	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	6	19	theme	Sj	895:896	arg1	dextran					901:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	2	20	theme	cibaria	307:313	arg1	1b					318:319	Weissella cibaria Sj 1b	297:319	Weissella cibaria Sj 1b	297:319	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	2	21	theme	protein	360:366	arg1	FPI					377:379	FPI	377:379	FPI	377:379	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	2	21	theme	protein	360:366	arg1	isolate					368:374	fava bean protein isolate	350:374	fava bean protein isolate (FPI)	350:380	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	0	22	theme	bean	26:29	arg1	protein					31:37	fava bean protein	21:37	fava bean protein	21:37	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	4	23	theme	mixture	639:645	arg1	incubation					609:618	Dry-heating incubation	597:618	Dry-heating incubation of FPI and dextran mixture	597:645	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	7	24	theme	20193	990:994	arg1	dextran					996:1002	DSM 20193 dextran	986:1002	DSM 20193 dextran	986:1002	pseudomesenteroides DSM 20193 dextran.					
29628253	2	25	theme	Weissella	297:305	arg1	1b					318:319	Weissella cibaria Sj 1b	297:319	Weissella cibaria Sj 1b	297:319	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	3	26	theme	isolated	424:431	arg1	dextrans					433:440	The two isolated dextrans	416:440	The two isolated dextrans	416:440	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	0	27	theme	fava	21:24	arg1	protein					31:37	fava bean protein	21:37	fava bean protein	21:37	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	6	28	theme	W.	884:885	arg1	dextran					901:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	0	29	theme	Sj	128:129	arg1	1b					131:132	Weissella cibaria Sj 1b	110:132	Weissella cibaria Sj 1b	110:132	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	4	30	theme	Dry-heating	597:607	arg1	incubation					609:618	Dry-heating incubation	597:618	Dry-heating incubation of FPI and dextran mixture	597:645	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	5	31	theme	bonds	833:837	arg1	influences					799:808	different influences	789:808	different influences of the formed covalent bonds on rheological properties	789:863	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	8	32	theme	FPI-dextran	1024:1034	arg1	interactions					1036:1047	The intermolecular FPI-dextran interactions	1005:1047	The intermolecular FPI-dextran interactions	1005:1047	The intermolecular FPI-dextran interactions played an important role in stabilizing the mixed systems at different pH.					
29628253	3	33	theme	few	523:525	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	33	theme	few	523:525	arg1	branches					537:544	few α-(1 → 3) branches	523:544	few α-(1 → 3) branches	523:544	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	5	34	theme	different	789:797	arg1	influences					799:808	different influences	789:808	different influences of the formed covalent bonds on rheological properties	789:863	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	7	35	theme	DSM	986:988	arg1	dextran					996:1002	DSM 20193 dextran	986:1002	DSM 20193 dextran	986:1002	pseudomesenteroides DSM 20193 dextran.					
29628253	2	36	theme	different	397:405	arg1	buffers					407:413	different buffers	397:413	different buffers	397:413	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	6	37	theme	cibaria	887:893	arg1	dextran					901:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	3	38	theme	typical	454:460	arg1	linkages					498:505	mainly α-(1 → 6) linkages	481:505	mainly α-(1 → 6) linkages (above 95%)	481:517	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	38	theme	typical	454:460	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	38	theme	typical	454:460	arg1	branches					537:544	few α-(1 → 3) branches	523:544	few α-(1 → 3) branches	523:544	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	8	39	theme	intermolecular	1009:1022	arg1	interactions					1036:1047	The intermolecular FPI-dextran interactions	1005:1047	The intermolecular FPI-dextran interactions	1005:1047	The intermolecular FPI-dextran interactions played an important role in stabilizing the mixed systems at different pH.					
29628253	3	40	theme	dextran	462:468	arg1	linkages					498:505	mainly α-(1 → 6) linkages	481:505	mainly α-(1 → 6) linkages (above 95%)	481:517	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	40	theme	dextran	462:468	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	40	theme	dextran	462:468	arg1	branches					537:544	few α-(1 → 3) branches	523:544	few α-(1 → 3) branches	523:544	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	4	41	theme	Maillard	705:712	arg1	reaction					714:721	the Maillard reaction	701:721	the Maillard reaction	701:721	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	8	42	theme	important	1059:1067	arg1	role					1069:1072	an important role	1056:1072	an important role	1056:1072	The intermolecular FPI-dextran interactions played an important role in stabilizing the mixed systems at different pH.					
29628253	3	43	theme	α-	527:528	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	43	theme	α-	527:528	arg1	branches					537:544	few α-(1 → 3) branches	523:544	few α-(1 → 3) branches	523:544	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	4	44	theme	dextran	631:637	arg1	mixture					639:645	dextran mixture	631:645	dextran mixture	631:645	Dry-heating incubation of FPI and dextran mixture facilitated the conjugation of dextran to FPI through the Maillard reaction.					
29628253	6	45	contain	had	909:911	arg2	ability					945:951	a much higher gel-strengthening ability	913:951	a much higher gel-strengthening ability	913:951	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	6	45	contain	had	909:911	arg1	dextran					901:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran	880:907	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	1	46	theme	fava	207:210	arg1	protein					217:223	fava bean protein	207:223	fava bean protein	207:223	The aim of this study was to study the interactions between dextran and fava bean protein.					
29628253	5	47	theme	covalent	824:831	arg1	bonds					833:837	the formed covalent bonds	813:837	the formed covalent bonds	813:837	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	3	48	theme	α-	488:489	arg1	linkages					498:505	mainly α-(1 → 6) linkages	481:505	mainly α-(1 → 6) linkages (above 95%)	481:517	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	48	theme	α-	488:489	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	48	theme	α-	488:489	arg1	%					516:516	above 95%	508:516	above 95%	508:516	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	1	49	theme	bean	212:215	arg1	protein					217:223	fava bean protein	207:223	fava bean protein	207:223	The aim of this study was to study the interactions between dextran and fava bean protein.					
29628253	0	50	theme	Leuconostoc	64:74	arg1	20193					100:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Leuconostoc pseudomesenteroides DSM 20193	64:104	Interactions between fava bean protein and dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b.					
29628253	3	51	theme	molar	568:572	arg1	mass					574:577	molar mass	568:577	molar mass	568:577	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	5	52	theme	conjugated	739:748	arg1	systems					750:756	conjugated systems	739:756	conjugated systems	739:756	Both mixed and conjugated systems were further heat-treated, and different influences of the formed covalent bonds on rheological properties were observed.					
29628253	2	53	theme	bean	355:358	arg1	FPI					377:379	FPI	377:379	FPI	377:379	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	2	53	theme	bean	355:358	arg1	isolate					368:374	fava bean protein isolate	350:374	fava bean protein isolate (FPI)	350:380	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	6	54	theme	higher	920:925	arg1	ability					945:951	a much higher gel-strengthening ability	913:951	a much higher gel-strengthening ability	913:951	The W. cibaria Sj 1b dextran had a much higher gel-strengthening ability than the Ln.					
29628253	3	55	theme	1 → 3	530:534	arg1	structure					470:478	a typical dextran structure	452:478	a typical dextran structure	452:478	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	3	55	theme	1 → 3	530:534	arg1	branches					537:544	few α-(1 → 3) branches	523:544	few α-(1 → 3) branches	523:544	The two isolated dextrans presented a typical dextran structure, mainly α-(1 → 6) linkages (above 95%) and few α-(1 → 3) branches, but they differed in molar mass and conformation.					
29628253	2	56	theme	fava	350:353	arg1	FPI					377:379	FPI	377:379	FPI	377:379	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
29628253	2	56	theme	fava	350:353	arg1	isolate					368:374	fava bean protein isolate	350:374	fava bean protein isolate (FPI)	350:380	Two dextrans produced by Leuconostoc pseudomesenteroides DSM 20193 and Weissella cibaria Sj 1b were purified and mixed with fava bean protein isolate (FPI) in water or in different buffers.					
31761409	3	0	theme	Fc	763:764	arg1	domains					766:772	Fc domains	763:772	Fc domains	763:772	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	2	1	theme	mAbs	393:396	arg1	glycosylation					376:388	Fc glycosylation	373:388	Fc glycosylation of mAbs	373:396	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	7	2	theme	mAbs	1537:1540	arg1	thermostability					1518:1532	thermostability	1518:1532	thermostability of mAbs and ADCs with homogeneous glycoforms	1518:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	1	3	theme	biotherapeutics	266:280	arg1	property					254:261	a critical property	243:261	a critical property of biotherapeutics	243:280	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	2	4	theme	glycosylation	508:520	arg1	lack					488:491	the lack	484:491	the lack of homogeneous glycosylation for comparison	484:535	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	7	5	theme	ADCs	1546:1549	arg1	thermostability					1518:1532	thermostability	1518:1532	thermostability of mAbs and ADCs with homogeneous glycoforms	1518:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	4	6	theme	high-mannose-type	891:907	arg1	N-glycans					909:917	high-mannose-type N-glycans	891:917	high-mannose-type N-glycans	891:917	The results revealed that high-mannose-type N-glycans dramatically reduce the Tm value of Fc, compared to complex-type N-glycans.					
31761409	7	7	from	analysis	1506:1513	arg1	thermostability					1518:1532	thermostability	1518:1532	thermostability of mAbs and ADCs with homogeneous glycoforms	1518:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	5	8	theme	cytotoxicity	1275:1286	arg1	advantage					1220:1228	the advantage	1216:1228	the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC)	1216:1293	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	0	9	theme	scanning	124:131	arg1	analysis					145:152	differential scanning flouremitry analysis	111:152	differential scanning flouremitry analysis	111:152	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	5	10	theme	afucosylated	1185:1196	arg1	mAbs					1198:1201	afucosylated mAbs	1185:1201	afucosylated mAbs	1185:1201	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	6	11	theme	lysine-linked	1312:1324	arg1	ADCs					1349:1352	lysine-linked and glycosite-specific ADCs	1312:1352	lysine-linked and glycosite-specific ADCs	1312:1352	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	7	12	theme	glycoengineered	1628:1642	arg1	mAbs					1644:1647	glycoengineered mAbs	1628:1647	glycoengineered mAbs	1628:1647	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	0	13	theme	differential	111:122	arg1	analysis					145:152	differential scanning flouremitry analysis	111:152	differential scanning flouremitry analysis	111:152	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	5	14	theme	N-glycan	1136:1143	arg1	end					1117:1119	non-reducing end	1104:1119	non-reducing end of biantennary N-glycan	1104:1143	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	6	15	theme	ADCs	1349:1352	arg1	analysis					1300:1307	DSF analysis	1296:1307	DSF analysis of lysine-linked and glycosite-specific ADCs	1296:1352	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	7	16	theme	in-depth	1497:1504	arg1	analysis					1506:1513	an in-depth analysis	1494:1513	an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms	1494:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	7	17	theme	glycosite-specific	1653:1670	arg1	ADCs					1672:1675	glycosite-specific ADCs	1653:1675	glycosite-specific ADCs using unnatural glycan and stabilized linkage	1653:1721	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	2	18	theme	precise	542:548	arg1	impact					550:555	the precise impact	538:555	the precise impact of glycoforms on thermostability of mAbs and ADCs	538:605	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	6	19	theme	ADCs	1402:1405	arg1	thermostability					1369:1383	thermostability	1369:1383	thermostability of glycan-linked ADCs	1369:1405	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	2	20	theme	antibody	422:429	arg1	thermostability					451:465	thermostability	451:465	thermostability	451:465	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	2	20	theme	antibody	422:429	arg1	functions					431:439	antibody functions	422:439	antibody functions including thermostability	422:465	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	0	21	theme	flouremitry	133:143	arg1	analysis					145:152	differential scanning flouremitry analysis	111:152	differential scanning flouremitry analysis	111:152	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	2	22	theme	homogeneous	496:506	arg1	glycosylation					508:520	homogeneous glycosylation	496:520	homogeneous glycosylation	496:520	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	3	23	theme	N-glycan	829:836	arg1	structures					838:847	well-defined N-glycan structures	816:847	well-defined N-glycan structures	816:847	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	5	24	gly	afucosylated	1185:1196	arg1	mAbs					1198:1201	afucosylated mAbs	1185:1201	afucosylated mAbs	1185:1201	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	1	25	theme	antibody-drug	207:219	arg1	conjugates					221:230	antibody-drug conjugates	207:230	antibody-drug conjugates (ADCs)	207:237	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	1	25	theme	antibody-drug	207:219	arg1	ADCs					233:236	ADCs	233:236	ADCs	233:236	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	5	26	theme	antibody-dependent	1242:1259	arg1	ADCC					1289:1292	ADCC	1289:1292	ADCC	1289:1292	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	5	26	theme	antibody-dependent	1242:1259	arg1	cytotoxicity					1275:1286	enhanced antibody-dependent cell-mediated cytotoxicity	1233:1286	enhanced antibody-dependent cell-mediated cytotoxicity (ADCC)	1233:1293	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	5	27	theme	enhanced	1233:1240	arg1	ADCC					1289:1292	ADCC	1289:1292	ADCC	1289:1292	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	5	27	theme	enhanced	1233:1240	arg1	cytotoxicity					1275:1286	enhanced antibody-dependent cell-mediated cytotoxicity	1233:1286	enhanced antibody-dependent cell-mediated cytotoxicity (ADCC)	1233:1293	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	5	28	from	modification	1088:1099	arg1	end					1117:1119	non-reducing end	1104:1119	non-reducing end of biantennary N-glycan	1104:1143	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	2	29	gly	glycosylation	376:388	arg1	mAbs					393:396	mAbs	393:396	mAbs	393:396	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	1	30	theme	conjugates	221:230	arg1	Thermostability					155:169	Thermostability	155:169	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs)	155:237	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	0	31	theme	Thermostability	0:14	arg1	detection					16:24	Thermostability detection	0:24	Thermostability detection	0:24	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	5	32	theme	cell-mediated	1261:1273	arg1	ADCC					1289:1292	ADCC	1289:1292	ADCC	1289:1292	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	5	32	theme	cell-mediated	1261:1273	arg1	cytotoxicity					1275:1286	enhanced antibody-dependent cell-mediated cytotoxicity	1233:1286	enhanced antibody-dependent cell-mediated cytotoxicity (ADCC)	1233:1293	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	3	33	theme	differential	685:696	arg1	DSF					720:722	DSF	720:722	DSF	720:722	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	3	33	theme	differential	685:696	arg1	fluorimetry					707:717	differential scanning fluorimetry	685:717	differential scanning fluorimetry (DSF)	685:723	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	6	34	theme	glycosite-specific	1330:1347	arg1	ADCs					1349:1352	lysine-linked and glycosite-specific ADCs	1312:1352	lysine-linked and glycosite-specific ADCs	1312:1352	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	6	35	link	lysine-linked	1312:1324	arg1	ADCs					1349:1352	lysine-linked and glycosite-specific ADCs	1312:1352	lysine-linked and glycosite-specific ADCs	1312:1352	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	2	36	theme	crucial	406:412	arg1	role					414:417	a crucial role	404:417	a crucial role	404:417	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	3	37	theme	glycoengineered	775:789	arg1	mAbs					791:794	glycoengineered mAbs	775:794	glycoengineered mAbs	775:794	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	1	38	theme	physicochemical	306:320	arg1	processes					322:330	their physicochemical processes	300:330	their physicochemical processes	300:330	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	4	39	theme	Fc	955:956	arg1	value					946:950	the Tm value	939:950	the Tm value of Fc	939:956	The results revealed that high-mannose-type N-glycans dramatically reduce the Tm value of Fc, compared to complex-type N-glycans.					
31761409	5	40	theme	non-reducing	1104:1115	arg1	end					1117:1119	non-reducing end	1104:1119	non-reducing end of biantennary N-glycan	1104:1143	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	0	41	theme	glycoengineered	46:60	arg1	antibodies					62:71	glycoengineered antibodies	46:71	glycoengineered antibodies	46:71	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	3	42	theme	domains	766:772	arg1	thermostability					744:758	the thermostability	740:758	the thermostability of Fc domains, glycoengineered mAbs, and ADCs	740:804	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	7	43	theme	homogeneous	1556:1566	arg1	glycoforms					1568:1577	homogeneous glycoforms	1556:1577	homogeneous glycoforms	1556:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	5	44	theme	mAbs	1198:1201	arg1	stability					1172:1180	the reduced stability	1160:1180	the reduced stability of afucosylated mAbs	1160:1201	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	2	45	theme	glycoforms	560:569	arg1	impact					550:555	the precise impact	538:555	the precise impact of glycoforms on thermostability of mAbs and ADCs	538:605	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	7	46	theme	new	1598:1600	arg1	strategies					1602:1611	new strategies	1598:1611	new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage	1598:1721	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	5	47	theme	mAbs	1064:1067	arg1	thermostability					1045:1059	the thermostability	1041:1059	the thermostability of mAbs	1041:1067	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	3	48	theme	ADCs	801:804	arg1	thermostability					744:758	the thermostability	740:758	the thermostability of Fc domains, glycoengineered mAbs, and ADCs	740:804	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	4	49	theme	Tm	943:944	arg1	value					946:950	the Tm value	939:950	the Tm value of Fc	939:956	The results revealed that high-mannose-type N-glycans dramatically reduce the Tm value of Fc, compared to complex-type N-glycans.					
31761409	5	50	theme	biantennary	1124:1134	arg1	N-glycan					1136:1143	biantennary N-glycan	1124:1143	biantennary N-glycan	1124:1143	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	0	51	theme	antibodies	62:71	arg1	optimization					30:41	optimization	30:41	optimization	30:41	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	0	51	theme	antibodies	62:71	arg1	detection					16:24	Thermostability detection	0:24	Thermostability detection	0:24	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	3	52	theme	mAbs	791:794	arg1	thermostability					744:758	the thermostability	740:758	the thermostability of Fc domains, glycoengineered mAbs, and ADCs	740:804	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	2	53	theme	ADCs	602:605	arg1	thermostability					574:588	thermostability	574:588	thermostability of mAbs and ADCs	574:605	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	3	54	theme	scanning	698:705	arg1	DSF					720:722	DSF	720:722	DSF	720:722	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	3	54	theme	scanning	698:705	arg1	fluorimetry					707:717	differential scanning fluorimetry	685:717	differential scanning fluorimetry (DSF)	685:723	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	5	55	theme	unnatural	1078:1086	arg1	modification					1088:1099	the unnatural modification	1074:1099	the unnatural modification on non-reducing end of biantennary N-glycan	1074:1143	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	2	56	theme	Fc	373:374	arg1	glycosylation					376:388	Fc glycosylation	373:388	Fc glycosylation of mAbs	373:396	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	7	57	with	thermostability	1518:1532	arg1	glycoforms					1568:1577	homogeneous glycoforms	1556:1577	homogeneous glycoforms	1556:1577	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	7	58	theme	stabilized	1704:1713	arg1	linkage					1715:1721	stabilized linkage	1704:1721	stabilized linkage	1704:1721	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	6	59	theme	glycan-linked	1388:1400	arg1	ADCs					1402:1405	glycan-linked ADCs	1388:1405	glycan-linked ADCs	1388:1405	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	3	60	theme	fluorimetry	707:717	arg1	technique					672:680	the technique	668:680	the technique of differential scanning fluorimetry (DSF)	668:723	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	6	61	theme	DSF	1296:1298	arg1	analysis					1300:1307	DSF analysis	1296:1307	DSF analysis of lysine-linked and glycosite-specific ADCs	1296:1352	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	3	62	theme	well-defined	816:827	arg1	structures					838:847	well-defined N-glycan structures	816:847	well-defined N-glycan structures	816:847	In this paper, we employed the technique of differential scanning fluorimetry (DSF) to investigate the thermostability of Fc domains, glycoengineered mAbs, and ADCs, carrying well-defined N-glycan structures for comparison.					
31761409	2	63	theme	mAbs	593:596	arg1	thermostability					574:588	thermostability	574:588	thermostability of mAbs and ADCs	574:605	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	1	64	theme	monoclonal	174:183	arg1	mAbs					197:200	mAbs	197:200	mAbs	197:200	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	1	64	theme	monoclonal	174:183	arg1	antibodies					185:194	monoclonal antibodies	174:194	monoclonal antibodies (mAbs)	174:201	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	1	65	theme	critical	245:252	arg1	property					254:261	a critical property	243:261	a critical property of biotherapeutics	243:280	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	0	66	theme	conjugates	91:100	arg1	optimization					30:41	optimization	30:41	optimization	30:41	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	0	66	theme	conjugates	91:100	arg1	detection					16:24	Thermostability detection	0:24	Thermostability detection	0:24	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	2	67	from	impact	550:555	arg1	thermostability					574:588	thermostability	574:588	thermostability of mAbs and ADCs	574:605	Fc glycosylation of mAbs plays a crucial role in antibody functions including thermostability, however, due to the lack of homogeneous glycosylation for comparison, the precise impact of glycoforms on thermostability of mAbs and ADCs remains challenging to elucidate.					
31761409	6	68	theme	optimized	1456:1464	arg1	linkage					1466:1472	an optimized linkage	1453:1472	an optimized linkage	1453:1472	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	1	69	theme	antibodies	185:194	arg1	Thermostability					155:169	Thermostability	155:169	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs)	155:237	Thermostability of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), as a critical property of biotherapeutics, is important for their physicochemical processes, pharmacodynamics, and pharmacokinetics.					
31761409	0	70	theme	antibody-drug	77:89	arg1	conjugates					91:100	antibody-drug conjugates	77:100	antibody-drug conjugates	77:100	Thermostability detection and optimization of glycoengineered antibodies and antibody-drug conjugates based on differential scanning flouremitry analysis.					
31761409	5	71	theme	reduced	1164:1170	arg1	stability					1172:1180	the reduced stability	1160:1180	the reduced stability of afucosylated mAbs	1160:1201	We also found that core-fucose contributes to the thermostability of mAbs, and the unnatural modification on non-reducing end of biantennary N-glycan can compensate the reduced stability of afucosylated mAbs and maintain the advantage of enhanced antibody-dependent cell-mediated cytotoxicity (ADCC).					
31761409	7	72	theme	unnatural	1683:1691	arg1	glycan					1693:1698	unnatural glycan	1683:1698	unnatural glycan	1683:1698	This work provides an in-depth analysis on thermostability of mAbs and ADCs with homogeneous glycoforms, and also proposes new strategies for optimizing glycoengineered mAbs and glycosite-specific ADCs using unnatural glycan and stabilized linkage.					
31761409	6	73	link	glycan-linked	1388:1400	arg1	ADCs					1402:1405	glycan-linked ADCs	1388:1405	glycan-linked ADCs	1388:1405	DSF analysis of lysine-linked and glycosite-specific ADCs indicated that thermostability of glycan-linked ADCs is reduced, but it could be improved by using an optimized linkage.					
31761409	4	74	theme	complex-type	971:982	arg1	N-glycans					984:992	complex-type N-glycans	971:992	complex-type N-glycans	971:992	The results revealed that high-mannose-type N-glycans dramatically reduce the Tm value of Fc, compared to complex-type N-glycans.					
31289257	5	0	theme	affinity	795:802	arg1	chromatography					804:817	Subsequent lectin affinity chromatography	777:817	Subsequent lectin affinity chromatography coupled with mass spectrometry	777:848	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	1	1	theme	major	141:145	arg1	implications					147:158	major implications	141:158	major implications for human diseases	141:177	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	7	2	theme	Gal-	1267:1270	arg1	-GalNAc					1278:1284	Gal-(β-1,3)-GalNAc	1267:1284	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	3	dep	study	1214:1218	arg1	show					1310:1313	show	1310:1313	to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD	1307:1396	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	3	dep	study	1214:1218	arg1	described					1223:1231	described	1223:1231	to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides	1220:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	4	4	theme	exposed	725:731	arg1	-GalNAc					744:750	exposed Gal-(β-1,3)-GalNAc	725:750	exposed Gal-(β-1,3)-GalNAc linked to glycoproteins	725:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	7	5	theme	β-1,3	1272:1276	arg1	-GalNAc					1278:1284	Gal-(β-1,3)-GalNAc	1267:1284	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	8	6	theme	therapeutic	1474:1484	arg1	targets					1486:1492	new therapeutic targets	1470:1492	new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers	1470:1558	These findings provide potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers.					
31289257	8	7	theme	prognostic	1538:1547	arg1	biomarkers					1549:1558	prognostic biomarkers	1538:1558	new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers	1470:1558	These findings provide potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers.					
31289257	3	8	theme	tyrosine	555:562	arg1	hydroxylase					564:574	tyrosine hydroxylase	555:574	tyrosine hydroxylase	555:574	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	6	9	dep	In	969:970	arg1	situ					972:975	situ	972:975	situ	972:975	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	5	10	from	glycosylation	868:880	arg1	mice					931:934	PD mice	928:934	PD mice	928:934	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	4	11	theme	Gal-	733:736	arg1	-GalNAc					744:750	exposed Gal-(β-1,3)-GalNAc	725:750	exposed Gal-(β-1,3)-GalNAc linked to glycoproteins	725:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	4	12	gly	glycoproteins	762:774	arg1	glycoproteins					762:774	glycoproteins	762:774	glycoproteins	762:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	4	13	theme	β-1,3	738:742	arg1	-GalNAc					744:750	exposed Gal-(β-1,3)-GalNAc	725:750	exposed Gal-(β-1,3)-GalNAc linked to glycoproteins	725:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	5	14	theme	protein	908:914	arg1	glycosylation					868:880	enhanced glycosylation	859:880	enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice	859:934	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	1	15	contain	has	137:139	arg1	glycosylation					111:123	Aberrant glycosylation	102:123	Aberrant glycosylation of proteins	102:135	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	1	15	contain	has	137:139	arg2	implications					147:158	major implications	141:158	major implications for human diseases	141:177	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	6	16	theme	complexes	1170:1178	arg1	structure					1152:1160	structure	1152:1160	structure	1152:1160	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	6	16	theme	complexes	1170:1178	arg1	distribution					1135:1146	distribution	1135:1146	distribution	1135:1146	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	3	17	from	reductions	477:486	arg1	hydroxylase					564:574	tyrosine hydroxylase	555:574	tyrosine hydroxylase	555:574	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	17	from	reductions	477:486	arg1	5-hydroxytryptamine					531:549	5-hydroxytryptamine	531:549	5-hydroxytryptamine	531:549	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	17	from	reductions	477:486	arg1	markers					502:508	biological markers	491:508	biological markers	491:508	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	17	from	reductions	477:486	arg1	dopamine					521:528	dopamine	521:528	dopamine	521:528	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	2	18	theme	disease	270:276	arg1	pathogenesis					242:253	the pathogenesis	238:253	the pathogenesis of Parkinson's disease (PD)	238:281	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	1	19	theme	human	164:168	arg1	diseases					170:177	human diseases	164:177	human diseases	164:177	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	5	20	theme	Subsequent	777:786	arg1	chromatography					804:817	Subsequent lectin affinity chromatography	777:817	Subsequent lectin affinity chromatography coupled with mass spectrometry	777:848	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	5	21	theme	enhanced	859:866	arg1	glycosylation					868:880	enhanced glycosylation	859:880	enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice	859:934	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	2	22	theme	mouse	286:290	arg1	model					292:296	a mouse model	284:296	a mouse model of PD	284:302	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	5	23	theme	PD	928:929	arg1	mice					931:934	PD mice	928:934	PD mice	928:934	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	0	24	theme	protein	31:37	arg1	Role					0:3	Role	0:3	Role of microtubule-associated protein 6	0:39	Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.					
31289257	8	25	theme	PD	1515:1516	arg1	treatment					1502:1510	the treatment	1498:1510	the treatment of PD	1498:1516	These findings provide potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers.					
31289257	5	26	theme	mass	832:835	arg1	spectrometry					837:848	mass spectrometry	832:848	mass spectrometry	832:848	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	3	27	from	aggregation	592:602	arg1	hydroxylase					564:574	tyrosine hydroxylase	555:574	tyrosine hydroxylase	555:574	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	27	from	aggregation	592:602	arg1	5-hydroxytryptamine					531:549	5-hydroxytryptamine	531:549	5-hydroxytryptamine	531:549	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	27	from	aggregation	592:602	arg1	markers					502:508	biological markers	491:508	biological markers	491:508	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	27	from	aggregation	592:602	arg1	dopamine					521:528	dopamine	521:528	dopamine	521:528	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	5	28	theme	microtubule-associated	885:906	arg1	protein					908:914	microtubule-associated protein 6	885:916	microtubule-associated protein 6 (MAP6)	885:923	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	5	28	theme	microtubule-associated	885:906	arg1	MAP6					919:922	MAP6	919:922	MAP6	919:922	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	0	29	theme	microtubule-associated	8:29	arg1	protein					31:37	microtubule-associated protein 6	8:39	microtubule-associated protein 6	8:39	Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.					
31289257	0	30	from	-GalNAc	70:76	arg1	disease					93:99	Parkinson's disease	81:99	Parkinson's disease	81:99	Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.					
31289257	7	31	theme	-GalNAc	1278:1284	arg1	oligosaccharides					1286:1301	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	1	32	theme	proteins	128:135	arg1	glycosylation					111:123	Aberrant glycosylation	102:123	Aberrant glycosylation of proteins	102:135	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	3	33	theme	PD-like	402:408	arg1	features					410:417	PD-like features	402:417	PD-like features	402:417	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	7	34	theme	MAP6	1342:1345	arg1	hyperglycosylation					1320:1337	hyperglycosylation	1320:1337	hyperglycosylation of MAP6	1320:1345	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	4	35	theme	agaracus	674:681	arg1	lectin					692:697	biotinylated agaracus bisporus lectin	661:697	biotinylated agaracus bisporus lectin	661:697	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	3	36	theme	motor	445:449	arg1	impairment					451:460	motor impairment	445:460	motor impairment	445:460	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	37	theme	features	410:417	arg1	Induction					389:397	Induction	389:397	Induction of PD-like features	389:417	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	4	38	attach	linked	752:757	arg2	-GalNAc					744:750	exposed Gal-(β-1,3)-GalNAc	725:750	exposed Gal-(β-1,3)-GalNAc linked to glycoproteins	725:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	4	38	attach	linked	752:757	arg1	glycoproteins					762:774	glycoproteins	762:774	glycoproteins	762:774	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	1	39	gly	glycosylation	111:123	arg1	proteins					128:135	proteins	128:135	proteins	128:135	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	3	40	theme	α-synuclein	607:617	arg1	reductions					477:486	reductions	477:486	reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase	477:574	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	40	theme	α-synuclein	607:617	arg1	aggregation					592:602	the aggregation	588:602	the aggregation of α-synuclein	588:617	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	6	41	gly	glycosylated	1055:1066	arg1	MAP6					1047:1050	MAP6	1047:1050	MAP6	1047:1050	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	4	42	theme	biotinylated	661:672	arg1	lectin					692:697	biotinylated agaracus bisporus lectin	661:697	biotinylated agaracus bisporus lectin	661:697	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	0	43	theme	Gal-	59:62	arg1	-GalNAc					70:76	Gal-(β-1,3)-GalNAc	59:76	Gal-(β-1,3)-GalNAc in Parkinson's disease	59:99	Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.					
31289257	6	44	theme	co-immunofluorescence	982:1002	arg1	analysis					1004:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	7	45	gly	hyperglycosylation	1320:1337	arg1	MAP6					1342:1345	MAP6	1342:1345	MAP6	1342:1345	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	5	46	theme	lectin	788:793	arg1	chromatography					804:817	Subsequent lectin affinity chromatography	777:817	Subsequent lectin affinity chromatography coupled with mass spectrometry	777:848	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	8	47	theme	new	1470:1472	arg1	targets					1486:1492	new therapeutic targets	1470:1492	new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers	1470:1558	These findings provide potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers.					
31289257	3	48	theme	biological	491:500	arg1	hydroxylase					564:574	tyrosine hydroxylase	555:574	tyrosine hydroxylase	555:574	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	48	theme	biological	491:500	arg1	5-hydroxytryptamine					531:549	5-hydroxytryptamine	531:549	5-hydroxytryptamine	531:549	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	48	theme	biological	491:500	arg1	markers					502:508	biological markers	491:508	biological markers	491:508	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	3	48	theme	biological	491:500	arg1	dopamine					521:528	dopamine	521:528	dopamine	521:528	Induction of PD-like features was verified by assessing motor impairment and confirming reductions in biological markers, including dopamine, 5-hydroxytryptamine and tyrosine hydroxylase, as well as the aggregation of α-synuclein.					
31289257	6	49	theme	dual	977:980	arg1	analysis					1004:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	2	50	theme	protein	201:207	arg1	glycosylation					209:221	protein glycosylation	201:221	protein glycosylation	201:221	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	5	51	gly	glycosylation	868:880	arg1	mice					931:934	PD mice	928:934	PD mice	928:934	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	5	51	gly	glycosylation	868:880	arg1	protein					908:914	microtubule-associated protein 6	885:916	microtubule-associated protein 6 (MAP6)	885:923	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	5	51	gly	glycosylation	868:880	arg1	MAP6					919:922	MAP6	919:922	MAP6	919:922	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	1	52	theme	Aberrant	102:109	arg1	glycosylation					111:123	Aberrant glycosylation	102:123	Aberrant glycosylation of proteins	102:135	Aberrant glycosylation of proteins has major implications for human diseases.					
31289257	7	53	theme	first	1208:1212	arg1	study					1214:1218	the first study	1204:1218	the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD	1204:1396	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	53	theme	first	1208:1212	arg1	This					1196:1199	This	1196:1199	This	1196:1199	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	6	54	dep	distribution	1135:1146	arg1	the					1131:1133	the	1131:1133	the	1131:1133	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	2	55	theme	PD	301:302	arg1	model					292:296	a mouse model	284:296	a mouse model of PD	284:302	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	8	56	theme	valuable	1434:1441	arg1	information					1443:1453	potentially valuable information	1422:1453	potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers	1422:1558	These findings provide potentially valuable information for developing new therapeutic targets for the treatment of PD as well as reliably prognostic biomarkers.					
31289257	4	57	theme	Altered	620:626	arg1	glycosylation					628:640	Altered glycosylation	620:640	Altered glycosylation	620:640	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	5	58	theme	healthy	951:957	arg1	controls					959:966	healthy controls	951:966	healthy controls	951:966	Subsequent lectin affinity chromatography coupled with mass spectrometry revealed enhanced glycosylation of microtubule-associated protein 6 (MAP6) in PD mice as compared to healthy controls.					
31289257	7	59	gly	glycoprotein	1243:1254	arg1	MAP6					1233:1236	MAP6	1233:1236	MAP6	1233:1236	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	59	gly	glycoprotein	1243:1254	arg1	glycoprotein					1243:1254	a glycoprotein	1241:1254	a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides	1241:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	6	60	theme	MAP6	1165:1168	arg1	complexes					1170:1178	MAP6 complexes	1165:1178	MAP6 complexes	1165:1178	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	6	61	theme	β-1,3	1078:1082	arg1	-GalNAc					1084:1090	Gal-(β-1,3)-GalNAc	1073:1090	Gal-(β-1,3)-GalNAc oligosaccharides	1073:1107	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	0	62	theme	β-1,3	64:68	arg1	-GalNAc					70:76	Gal-(β-1,3)-GalNAc	59:76	Gal-(β-1,3)-GalNAc in Parkinson's disease	59:99	Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.					
31289257	6	63	theme	-GalNAc	1084:1090	arg1	oligosaccharides					1092:1107	Gal-(β-1,3)-GalNAc oligosaccharides	1073:1107	Gal-(β-1,3)-GalNAc oligosaccharides	1073:1107	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	7	64	contain	containing	1256:1265	arg1	MAP6					1233:1236	MAP6	1233:1236	MAP6	1233:1236	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	64	contain	containing	1256:1265	arg1	glycoprotein					1243:1254	a glycoprotein	1241:1254	a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides	1241:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	7	64	contain	containing	1256:1265	arg2	oligosaccharides					1286:1301	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	Gal-(β-1,3)-GalNAc oligosaccharides	1267:1301	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	2	65	theme	1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine	336:379	arg1	injection					323:331	injection	323:331	injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)	323:386	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	4	66	theme	bisporus	683:690	arg1	lectin					692:697	biotinylated agaracus bisporus lectin	661:697	biotinylated agaracus bisporus lectin	661:697	Altered glycosylation was detected using biotinylated agaracus bisporus lectin, which specifically binds exposed Gal-(β-1,3)-GalNAc linked to glycoproteins.					
31289257	2	67	gly	glycosylation	209:221	arg1	PD					279:280	PD	279:280	PD	279:280	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	2	67	gly	glycosylation	209:221	arg1	disease					270:276	Parkinson's disease	258:276	Parkinson's disease (PD)	258:281	To determine whether protein glycosylation contributes to the pathogenesis of Parkinson's disease (PD), a mouse model of PD was established by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).					
31289257	6	68	theme	In	969:970	arg1	analysis					1004:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis	969:1011	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
31289257	7	69	theme	PD	1395:1396	arg1	pathogenesis					1379:1390	the pathogenesis	1375:1390	the pathogenesis of PD	1375:1396	This is the first study to described MAP6 as a glycoprotein containing Gal-(β-1,3)-GalNAc oligosaccharides and to show that hyperglycosylation of MAP6 is strongly associated with the pathogenesis of PD.					
31289257	6	70	theme	Gal-	1073:1076	arg1	-GalNAc					1084:1090	Gal-(β-1,3)-GalNAc	1073:1090	Gal-(β-1,3)-GalNAc oligosaccharides	1073:1107	In situ dual co-immunofluorescence analysis and immunoblotting confirmed that MAP6 is glycosylated with Gal-(β-1,3)-GalNAc oligosaccharides, which in turn alters the distribution and structure of MAP6 complexes within neurons.					
29897488	4	0	theme	fluorescent	988:998	arg1	detection					1000:1008	fluorescent detection	988:1008	fluorescent detection	988:1008	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	6	1	theme	glycan	1389:1394	arg1	analysis					1396:1403	detailed glycan analysis	1380:1403	detailed glycan analysis	1380:1403	GlycoStore aims to support detailed glycan analysis by providing a resource that underpins current workflows.					
29897488	2	2	theme	available	354:362	arg1	datasets					377:384	publicly available experimental datasets	345:384	publicly available experimental datasets	345:384	The database is built on publicly available experimental datasets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT).					
29897488	5	3	from	available	1237:1245	arg1	GlycoBase					1250:1258	GlycoBase	1250:1258	GlycoBase	1250:1258	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	6	4	theme	detailed	1380:1387	arg1	analysis					1396:1403	detailed glycan analysis	1380:1403	detailed glycan analysis	1380:1403	GlycoStore aims to support detailed glycan analysis by providing a resource that underpins current workflows.					
29897488	4	5	theme	chromatography	875:888	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	5	theme	chromatography	875:888	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	6	dep	chromatography	1048:1061	arg1	ii					1012:1013	ii	1012:1013	ii	1012:1013	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	7	theme	HILIC	891:895	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	7	theme	HILIC	891:895	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	8	theme	phase	965:969	arg1	-U/HPLC					975:981	reversed phase (RP)-U/HPLC	956:981	reversed phase (RP)-U/HPLC	956:981	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	9	with	combination	1066:1076	arg1	detection					1093:1101	ESI-MS/MS detection	1083:1101	ESI-MS/MS detection	1083:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	9	10	theme	Supplementary	1635:1647	arg1	data					1675:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data are available at Bioinformatics online.					
29897488	1	11	theme	curated	92:98	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	11	theme	curated	92:98	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	7	12	theme	project	1558:1564	arg1	partners					1566:1573	the project partners	1554:1573	the project partners	1554:1573	It will be regularly updated by expert annotation of published data and data obtained from the project partners.					
29897488	4	13	theme	reversed	956:963	arg1	-U/HPLC					975:981	reversed phase (RP)-U/HPLC	956:981	reversed phase (RP)-U/HPLC	956:981	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	14	theme	structure	771:779	arg1	entries					781:787	approximately 850 unique glycan structure entries	739:787	approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF)	739:1189	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	3	15	dep	published	574:582	arg1	collections					602:612	collections	602:612	collections	602:612	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	3	15	dep	published	574:582	arg1	University					682:691	University	682:691	University	682:691	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	3	15	dep	published	574:582	arg1	Ltd.					704:707	Ltd.	704:707	Ltd.	704:707	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	5	16	theme	many	1212:1215	arg1	features					1217:1224	many features	1212:1224	many features previously available in GlycoBase	1212:1258	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	4	17	theme	RP	972:973	arg1	-U/HPLC					975:981	reversed phase (RP)-U/HPLC	956:981	reversed phase (RP)-U/HPLC	956:981	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	5	18	from	GlycoBase	1250:1258	arg1	available					1237:1245	available	1237:1245	available	1237:1245	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	5	19	theme	popular	1335:1341	arg1	resource					1343:1350	this popular resource	1330:1350	this popular resource	1330:1350	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	1	20	theme	N-	179:180	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	20	theme	N-	179:180	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	21	theme	porous	1016:1021	arg1	chromatography					1048:1061	porous graphitized carbon (PGC) chromatography	1016:1061	(ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection	1011:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	22	theme	Summary	68:74	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	22	theme	Summary	68:74	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	5	23	theme	resource	1343:1350	arg1	model					1321:1325	model	1321:1325	model of this popular resource	1321:1350	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	5	23	theme	resource	1343:1350	arg1	collections					1305:1315	the data collections	1296:1315	the data collections	1296:1315	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	2	24	theme	National	461:468	arg1	Institute					470:478	the National Institute	457:478	the National Institute for Bioprocessing Research and Training (NIBRT)	457:526	The database is built on publicly available experimental datasets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT).					
29897488	8	25	dep	//www.glycostore.org	1613:1632	arg1	implementation					1593:1606	implementation	1593:1606	implementation	1593:1606	Availability and implementation http://www.glycostore.org.					
29897488	8	25	dep	//www.glycostore.org	1613:1632	arg1	Availability					1576:1587	Availability	1576:1587	Availability	1576:1587	Availability and implementation http://www.glycostore.org.					
29897488	4	26	theme	interaction	863:873	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	26	theme	interaction	863:873	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	27	theme	O-	183:184	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	27	theme	O-	183:184	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	3	28	dep	University	682:691	arg1	Macquarie					672:680	Macquarie	672:680	Macquarie	672:680	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	4	29	theme	hydrophilic	851:861	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	29	theme	hydrophilic	851:861	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	8	30	dep	Availability	1576:1587	arg1	http					1608:1611	http	1608:1611	http	1608:1611	Availability and implementation http://www.glycostore.org.					
29897488	4	31	with	chromatography	928:941	arg1	detection					1000:1008	fluorescent detection	988:1008	fluorescent detection	988:1008	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	9	32	theme	Bioinformatics	1697:1710	arg1	online					1712:1717	Bioinformatics online	1697:1717	Bioinformatics online	1697:1717	Supplementary information Supplementary data are available at Bioinformatics online.					
29897488	4	33	theme	carbon	1035:1040	arg1	chromatography					1048:1061	porous graphitized carbon (PGC) chromatography	1016:1061	(ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection	1011:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	34	theme	glycoproteins	272:284	arg1	range					263:267	a range	261:267	a range of glycoproteins, glycolipids and biotherapeutics	261:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	35	theme	glycan	764:769	arg1	entries					781:787	approximately 850 unique glycan structure entries	739:787	approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF)	739:1189	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	36	theme	glycosphingolipid	187:203	arg1	glycans					211:217	glycosphingolipid (GSL) glycans	187:217	glycosphingolipid (GSL) glycans	187:217	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	37	theme	retention	812:820	arg1	positions					822:830	8500 retention positions	807:830	8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser	807:1149	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	38	dep	composition	155:165	arg1	mass-spectrometry					137:153	mass-spectrometry	137:153	mass-spectrometry	137:153	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	5	39	theme	model	1321:1325	arg1	limitations					1281:1291	the limitations	1277:1291	the limitations of the data collections and model of this popular resource	1277:1350	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	5	40	theme	collections	1305:1315	arg1	limitations					1281:1291	the limitations	1277:1291	the limitations of the data collections and model of this popular resource	1277:1350	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	0	41	theme	retention	26:34	arg1	properties					36:45	retention properties	26:45	retention properties	26:45	GlycoStore: a database of retention properties for glycan analysis.					
29897488	1	42	theme	glycolipids	287:297	arg1	range					263:267	a range	261:267	a range of glycoproteins, glycolipids and biotherapeutics	261:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	43	theme	GSL	206:208	arg1	glycans					211:217	glycosphingolipid (GSL) glycans	187:217	glycosphingolipid (GSL) glycans	187:217	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	2	44	theme	Bioprocessing	484:496	arg1	Research					498:505	Bioprocessing Research	484:505	Bioprocessing Research	484:505	The database is built on publicly available experimental datasets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT).					
29897488	9	45	from	online	1712:1717	arg1	available					1684:1692	available	1684:1692	available	1684:1692	Supplementary information Supplementary data are available at Bioinformatics online.					
29897488	7	46	theme	data	1535:1538	arg1	annotation					1502:1511	expert annotation	1495:1511	expert annotation of published data and data obtained from the project partners	1495:1573	It will be regularly updated by expert annotation of published data and data obtained from the project partners.					
29897488	5	47	theme	available	1237:1245	arg1	features					1217:1224	many features	1212:1224	many features previously available in GlycoBase	1212:1258	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	1	48	gly	glycoproteins	272:284	arg1	glycoproteins					272:284	glycoproteins	272:284	glycoproteins	272:284	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	7	49	theme	published	1516:1524	arg1	data					1526:1529	published data	1516:1529	published data	1516:1529	It will be regularly updated by expert annotation of published data and data obtained from the project partners.					
29897488	1	50	theme	biotherapeutics	303:317	arg1	range					263:267	a range	261:267	a range of glycoproteins, glycolipids and biotherapeutics	261:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	51	theme	performance	909:919	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	51	theme	performance	909:919	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	52	theme	graphitized	1023:1033	arg1	chromatography					1048:1061	porous graphitized carbon (PGC) chromatography	1016:1061	(ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection	1011:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	3	53	theme	in-house	588:595	arg1	data					597:600	in-house data	588:600	in-house data	588:600	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	1	54	theme	chromatographic	100:114	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	54	theme	chromatographic	100:114	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	0	55	theme	properties	36:45	arg1	database					14:21	a database	12:21	GlycoStore: a database of retention properties for glycan analysis.	0:66	GlycoStore: a database of retention properties for glycan analysis.					
29897488	4	56	theme	ultra-high	898:907	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	56	theme	ultra-high	898:907	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	7	57	theme	data	1526:1529	arg1	annotation					1502:1511	expert annotation	1495:1511	expert annotation of published data and data obtained from the project partners	1495:1573	It will be regularly updated by expert annotation of published data and data obtained from the project partners.					
29897488	9	58	theme	information	1649:1659	arg1	data					1675:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data are available at Bioinformatics online.					
29897488	4	59	with	-U/HPLC	975:981	arg1	detection					1000:1008	fluorescent detection	988:1008	fluorescent detection	988:1008	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	3	60	theme	Bioprocessing	623:635	arg1	BTI					659:661	BTI	659:661	BTI	659:661	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	3	60	theme	Bioprocessing	623:635	arg1	Institute					648:656	the Bioprocessing Technology Institute	619:656	the Bioprocessing Technology Institute (BTI)	619:662	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	4	61	theme	8500	807:810	arg1	positions					822:830	8500 retention positions	807:830	8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser	807:1149	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	3	62	theme	Technology	637:646	arg1	BTI					659:661	BTI	659:661	BTI	659:661	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	3	62	theme	Technology	637:646	arg1	Institute					648:656	the Bioprocessing Technology Institute	619:656	the Bioprocessing Technology Institute (BTI)	619:662	It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd.					
29897488	1	63	theme	electrophoretic	117:131	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	63	theme	electrophoretic	117:131	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	64	theme	glycans	211:217	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	64	theme	glycans	211:217	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	65	theme	liquid	921:926	arg1	U/HPLC					944:949	U/HPLC	944:949	U/HPLC	944:949	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	65	theme	liquid	921:926	arg1	chromatography					928:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography	847:941	(i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC)	847:950	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	66	theme	unique	757:762	arg1	entries					781:787	approximately 850 unique glycan structure entries	739:787	approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF)	739:1189	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	2	67	theme	experimental	364:375	arg1	datasets					377:384	publicly available experimental datasets	345:384	publicly available experimental datasets	345:384	The database is built on publicly available experimental datasets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT).					
29897488	4	68	theme	ESI-MS/MS	1083:1091	arg1	detection					1093:1101	ESI-MS/MS detection	1083:1101	ESI-MS/MS detection	1083:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	69	dep	chromatography	928:941	arg1	i					848:848	i	848:848	i	848:848	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	69	dep	chromatography	928:941	arg1	chromatography					1048:1061	porous graphitized carbon (PGC) chromatography	1016:1061	(ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection	1011:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	69	dep	chromatography	928:941	arg1	electrophoresis					1124:1138	electrophoresis	1124:1138	electrophoresis	1124:1138	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	6	70	theme	current	1444:1450	arg1	workflows					1452:1460	current workflows	1444:1460	current workflows	1444:1460	GlycoStore aims to support detailed glycan analysis by providing a resource that underpins current workflows.					
29897488	9	71	theme	Supplementary	1661:1673	arg1	data					1675:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data	1635:1678	Supplementary information Supplementary data are available at Bioinformatics online.					
29897488	4	72	from	chromatography	1048:1061	arg1	combination					1066:1076	combination	1066:1076	combination with ESI-MS/MS detection	1066:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	5	73	theme	data	1300:1303	arg1	collections					1305:1315	the data collections	1296:1315	the data collections	1296:1315	GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource.					
29897488	1	74	theme	free	223:226	arg1	oligosaccharides					228:243	free oligosaccharides	223:243	free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	223:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	0	75	theme	glycan	51:56	arg1	analysis					58:65	glycan analysis	51:65	glycan analysis	51:65	GlycoStore: a database of retention properties for glycan analysis.					
29897488	7	76	theme	expert	1495:1500	arg1	annotation					1502:1511	expert annotation	1495:1511	expert annotation of published data and data obtained from the project partners	1495:1573	It will be regularly updated by expert annotation of published data and data obtained from the project partners.					
29897488	0	77	dep	GlycoStore	0:9	arg1	database					14:21	a database	12:21	GlycoStore: a database of retention properties for glycan analysis.	0:66	GlycoStore: a database of retention properties for glycan analysis.					
29897488	4	78	theme	fluorescence	1159:1170	arg1	CE-LIF					1183:1188	CE-LIF	1183:1188	CE-LIF	1183:1188	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	4	78	theme	fluorescence	1159:1170	arg1	detection					1172:1180	fluorescence detection	1159:1180	fluorescence detection (CE-LIF)	1159:1189	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	79	theme	composition	155:165	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	79	theme	composition	155:165	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	4	80	theme	PGC	1043:1045	arg1	chromatography					1048:1061	porous graphitized carbon (PGC) chromatography	1016:1061	(ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection	1011:1101	GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
29897488	1	81	theme	oligosaccharides	228:243	arg1	database					167:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database	90:174	a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics	90:317	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
29897488	1	81	theme	oligosaccharides	228:243	arg1	GlycoStore					76:85	Summary GlycoStore	68:85	Summary GlycoStore	68:85	Summary GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics.					
31058489	7	0	theme	pluripotent	1622:1632	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	0	theme	pluripotent	1622:1632	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	4	1	theme	reproducible	1010:1021	arg1	times					1033:1037	highly reproducible migration times	1003:1037	highly reproducible migration times	1003:1037	GSL glycans were detected with highly reproducible migration times after repeated analysis by xCGE-LIF.					
31058489	0	2	theme	Cells	210:214	arg1	Markers					176:182	Cell-Surface Markers	163:182	Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes	163:241	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	7	3	theme	well-known	1605:1614	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	3	theme	well-known	1605:1614	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	4	theme	lacto-	1550:1555	arg1	structures					1579:1588	lacto- and globo-series core structures	1550:1588	structures	1579:1588	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	0	5	theme	Derived	220:226	arg1	Cardiomyocytes					228:241	Derived Cardiomyocytes	220:241	Derived Cardiomyocytes	220:241	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	6	6	theme	pluripotent	1302:1312	arg1	cells					1319:1323	105 human induced pluripotent stem cells	1284:1323	105 human induced pluripotent stem cells	1284:1323	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	8	7	theme	abundant	1829:1836	arg1	GSLs					1838:1841	highly abundant GSLs	1822:1841	highly abundant GSLs	1822:1841	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	7	8	theme	core	1574:1577	arg1	structures					1579:1588	lacto- and globo-series core structures	1550:1588	structures	1579:1588	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	6	9	theme	human	1288:1292	arg1	cells					1319:1323	105 human induced pluripotent stem cells	1284:1323	105 human induced pluripotent stem cells	1284:1323	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	3	10	theme	multiplexed	870:880	arg1	electrophoresis					896:910	multiplexed capillary gel electrophoresis	870:910	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	870:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	6	11	theme	GSL	1380:1382	arg1	glycosylation					1384:1396	GSL glycosylation	1380:1396	GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs	1380:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	12	theme	stem	1427:1430	arg1	hiPSCs					1439:1444	hiPSCs	1439:1444	hiPSCs	1439:1444	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	12	theme	stem	1427:1430	arg1	cells					1432:1436	pluripotent stem cells	1415:1436	pluripotent stem cells (hiPSCs)	1415:1445	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	7	13	theme	general	1512:1518	arg1	switch					1520:1525	a general switch	1510:1525	a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3	1510:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	8	14	theme	cell	1850:1853	arg1	surface					1855:1861	the cell surface	1846:1861	the cell surface of stem cell-derived cardiomyocytes	1846:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	0	15	theme	Cell-Surface	163:174	arg1	Markers					176:182	Cell-Surface Markers	163:182	Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes	163:241	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	5	16	theme	fucosyl	1204:1210	arg1	lactotetra					1212:1221	fucosyl lactotetra	1204:1221	fucosyl lactotetra	1204:1221	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	1	17	theme	human	259:263	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	17	theme	human	259:263	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	4	18	theme	repeated	1045:1052	arg1	analysis					1054:1061	repeated analysis	1045:1061	repeated analysis by xCGE-LIF	1045:1073	GSL glycans were detected with highly reproducible migration times after repeated analysis by xCGE-LIF.					
31058489	6	19	theme	global	1358:1363	arg1	alterations					1365:1375	global alterations	1358:1375	global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs	1358:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	3	20	theme	gel	892:894	arg1	electrophoresis					896:910	multiplexed capillary gel electrophoresis	870:910	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	870:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	8	21	theme	cell-derived	1871:1882	arg1	cardiomyocytes					1884:1897	stem cell-derived cardiomyocytes	1866:1897	stem cell-derived cardiomyocytes	1866:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	6	22	theme	pluripotent	1415:1425	arg1	hiPSCs					1439:1444	hiPSCs	1439:1444	hiPSCs	1439:1444	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	22	theme	pluripotent	1415:1425	arg1	cells					1432:1436	pluripotent stem cells	1415:1436	pluripotent stem cells (hiPSCs)	1415:1445	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	0	23	theme	Pluripotent	193:203	arg1	Cells					210:214	Human Pluripotent Stem Cells	187:214	Human Pluripotent Stem Cells	187:214	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	6	24	gly	glycosylation	1384:1396	arg1	hiPSCs					1439:1444	hiPSCs	1439:1444	hiPSCs	1439:1444	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	24	gly	glycosylation	1384:1396	arg1	hiPSC-CMs					1451:1459	hiPSC-CMs	1451:1459	hiPSC-CMs	1451:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	24	gly	glycosylation	1384:1396	arg1	cells					1432:1436	pluripotent stem cells	1415:1436	pluripotent stem cells (hiPSCs)	1415:1445	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	3	25	theme	laser-induced	923:935	arg1	detection					950:958	laser-induced fluorescence detection	923:958	laser-induced fluorescence detection (xCGE-LIF)	923:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	3	25	theme	laser-induced	923:935	arg1	xCGE-LIF					961:968	xCGE-LIF	961:968	xCGE-LIF	961:968	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	0	26	theme	Capillary	73:81	arg1	Electrophoresis					87:101	Multiplexed Capillary Gel Electrophoresis	61:101	Multiplexed Capillary Gel Electrophoresis	61:101	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	1	27	theme	cell-surface	388:399	arg1	characterization					414:429	cell-surface marker-based characterization	388:429	cell-surface marker-based characterization	388:429	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	3	28	theme	workflow	724:731	arg1	development					670:680	the development	666:680	the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	666:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	7	29	from	GSLs	1540:1543	arg1	hiPSCs					1707:1712	hiPSCs	1707:1712	hiPSCs	1707:1712	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	29	from	GSLs	1540:1543	arg1	switch					1520:1525	a general switch	1510:1525	a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3	1510:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	6	30	theme	induced	1407:1413	arg1	hiPSCs					1439:1444	hiPSCs	1439:1444	hiPSCs	1439:1444	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	30	theme	induced	1407:1413	arg1	cells					1432:1436	pluripotent stem cells	1415:1436	pluripotent stem cells (hiPSCs)	1415:1445	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	2	31	theme	analytical	625:634	arg1	challenges					636:645	significant analytical challenges	613:645	significant analytical challenges	613:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	0	32	theme	Fluorescence	128:139	arg1	Detection					141:149	Laser-Induced Fluorescence Detection	114:149	Laser-Induced Fluorescence Detection	114:149	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	7	33	with	GSLs	1540:1543	arg1	structures					1579:1588	lacto- and globo-series core structures	1550:1588	structures	1579:1588	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	5	34	theme	different	1128:1136	arg1	species					1145:1151	38 different glycan species	1125:1151	38 different glycan species	1125:1151	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	6	35	theme	human	1401:1405	arg1	hiPSCs					1439:1444	hiPSCs	1439:1444	hiPSCs	1439:1444	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	35	theme	human	1401:1405	arg1	cells					1432:1436	pluripotent stem cells	1415:1436	pluripotent stem cells (hiPSCs)	1415:1445	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	2	36	theme	biomolecules	525:536	arg1	Glycosphingolipids					452:469	Glycosphingolipids	452:469	Glycosphingolipids (GSLs)	452:476	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	36	theme	biomolecules	525:536	arg1	family					484:489	a family	482:489	a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges	482:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	37	theme	diverse	501:507	arg1	biomolecules					525:536	highly diverse surface-exposed biomolecules	494:536	highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges	494:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	37	theme	diverse	501:507	arg1	markers					584:590	potential surface markers	566:590	potential surface markers for hiPSC-CMs due to significant analytical challenges	566:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	5	38	theme	time	1100:1103	arg1	database					1105:1112	a migration time database	1088:1112	a migration time database comprising 38 different glycan species	1088:1151	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	2	39	theme	potential	566:574	arg1	markers					584:590	potential surface markers	566:590	potential surface markers for hiPSC-CMs due to significant analytical challenges	566:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	39	theme	potential	566:574	arg1	biomolecules					525:536	highly diverse surface-exposed biomolecules	494:536	highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges	494:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	3	40	theme	glycans	765:771	arg1	analysis					741:748	the analysis	737:748	the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	737:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	0	41	theme	Glycosphingolipid	26:42	arg1	Glycosylation					44:56	Glycosphingolipid Glycosylation	26:56	Glycosphingolipid Glycosylation	26:56	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	1	42	theme	regenerative	355:366	arg1	medicine					368:375	regenerative medicine	355:375	regenerative medicine	355:375	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	7	43	from	SSEA4	1698:1702	arg1	hiPSCs					1707:1712	hiPSCs	1707:1712	hiPSCs	1707:1712	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	43	from	SSEA4	1698:1702	arg1	switch					1520:1525	a general switch	1510:1525	a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3	1510:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	44	theme	embryonic	1666:1674	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	44	theme	embryonic	1666:1674	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	4	45	theme	GSL	972:974	arg1	glycans					976:982	GSL glycans	972:982	GSL glycans	972:982	GSL glycans were detected with highly reproducible migration times after repeated analysis by xCGE-LIF.					
31058489	7	46	theme	marker	1644:1649	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	46	theme	marker	1644:1649	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	3	47	theme	glycanase	791:799	arg1	digestion					801:809	ceramide glycanase digestion	782:809	ceramide glycanase digestion	782:809	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	1	48	theme	induced	265:271	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	48	theme	induced	265:271	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	7	49	theme	stem	1634:1637	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	49	theme	stem	1634:1637	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	6	50	theme	glycan	1243:1248	arg1	profiling					1250:1258	GSL glycan profiling	1239:1258	GSL glycan profiling	1239:1258	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	1	51	theme	stem	285:288	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	51	theme	stem	285:288	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	7	52	theme	human	1616:1620	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	52	theme	human	1616:1620	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	3	53	theme	acid	844:847	arg1	labeling					856:863	8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling	812:863	8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling	812:863	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	6	54	theme	cells	1432:1436	arg1	glycosylation					1384:1396	GSL glycosylation	1380:1396	GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs	1380:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	1	55	theme	cardiomyocytes	303:316	arg1	Application					244:254	Application	244:254	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine	244:375	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	3	56	link	GSL-derived	753:763	arg1	glycans					765:771	GSL-derived glycans	753:771	GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	753:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	0	57	theme	Cardiomyocytes	228:241	arg1	Markers					176:182	Cell-Surface Markers	163:182	Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes	163:241	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	8	58	theme	GM3	1804:1806	arg1	This					1771:1774	This	1771:1774	This	1771:1774	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	8	58	theme	GM3	1804:1806	arg1	description					1789:1799	the first description	1779:1799	the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes	1779:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	4	59	theme	migration	1023:1031	arg1	times					1033:1037	highly reproducible migration times	1003:1037	highly reproducible migration times	1003:1037	GSL glycans were detected with highly reproducible migration times after repeated analysis by xCGE-LIF.					
31058489	6	60	theme	stem	1314:1317	arg1	cells					1319:1323	105 human induced pluripotent stem cells	1284:1323	105 human induced pluripotent stem cells	1284:1323	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	1	61	from	transplants	340:350	arg1	medicine					368:375	regenerative medicine	355:375	regenerative medicine	355:375	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	8	62	theme	GD3	1812:1814	arg1	This					1771:1774	This	1771:1774	This	1771:1774	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	8	62	theme	GD3	1812:1814	arg1	description					1789:1799	the first description	1779:1799	the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes	1779:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	7	63	theme	globo-series	1561:1572	arg1	structures					1579:1588	lacto- and globo-series core structures	1550:1588	structures	1579:1588	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	6	64	theme	induced	1294:1300	arg1	cells					1319:1323	105 human induced pluripotent stem cells	1284:1323	105 human induced pluripotent stem cells	1284:1323	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	6	65	theme	glycosylation	1384:1396	arg1	alterations					1365:1375	global alterations	1358:1375	global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs	1358:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	1	66	link	cell-derived	290:301	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	66	link	cell-derived	290:301	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	7	67	theme	complex	1532:1538	arg1	GSLs					1540:1543	complex GSLs	1532:1543	complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1532:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	3	68	theme	capillary	882:890	arg1	electrophoresis					896:910	multiplexed capillary gel electrophoresis	870:910	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	870:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	3	69	theme	novel	687:691	arg1	workflow					724:731	a novel and high-throughput-compatible workflow	685:731	a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	685:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	0	70	theme	Human	187:191	arg1	Cells					210:214	Human Pluripotent Stem Cells	187:214	Human Pluripotent Stem Cells	187:214	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	5	71	theme	lactotetra	1212:1221	arg1	pg					1198:1199	as few as 10 pg	1185:1199	as few as 10 pg of fucosyl lactotetra	1185:1221	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	7	72	theme	simple	1725:1730	arg1	GM3					1745:1747	GM3	1745:1747	GM3	1745:1747	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	72	theme	simple	1725:1730	arg1	gangliosides					1732:1743	the simple gangliosides GM3 and GD3	1721:1755	the simple gangliosides GM3 and GD3	1721:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	72	theme	simple	1725:1730	arg1	GD3					1753:1755	GD3	1753:1755	GD3	1753:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	0	73	theme	Stem	205:208	arg1	Cells					210:214	Human Pluripotent Stem Cells	187:214	Human Pluripotent Stem Cells	187:214	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	7	74	with	SSEA4	1698:1702	arg1	structures					1579:1588	lacto- and globo-series core structures	1550:1588	structures	1579:1588	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	0	75	theme	Gel	83:85	arg1	Electrophoresis					87:101	Multiplexed Capillary Gel Electrophoresis	61:101	Multiplexed Capillary Gel Electrophoresis	61:101	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	3	76	theme	high-throughput-compatible	697:722	arg1	workflow					724:731	a novel and high-throughput-compatible workflow	685:731	a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	685:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	8	77	theme	cardiomyocytes	1884:1897	arg1	surface					1855:1861	the cell surface	1846:1861	the cell surface of stem cell-derived cardiomyocytes	1846:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	3	78	theme	fluorescence	937:948	arg1	detection					950:958	laser-induced fluorescence detection	923:958	laser-induced fluorescence detection (xCGE-LIF)	923:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	3	78	theme	fluorescence	937:948	arg1	xCGE-LIF					961:968	xCGE-LIF	961:968	xCGE-LIF	961:968	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	1	79	theme	marker-based	401:412	arg1	characterization					414:429	cell-surface marker-based characterization	388:429	cell-surface marker-based characterization	388:429	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	2	80	theme	surface	576:582	arg1	markers					584:590	potential surface markers	566:590	potential surface markers for hiPSC-CMs due to significant analytical challenges	566:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	80	theme	surface	576:582	arg1	biomolecules					525:536	highly diverse surface-exposed biomolecules	494:536	highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges	494:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	0	81	theme	Laser-Induced	114:126	arg1	Detection					141:149	Laser-Induced Fluorescence Detection	114:149	Laser-Induced Fluorescence Detection	114:149	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	2	82	theme	significant	613:623	arg1	challenges					636:645	significant analytical challenges	613:645	significant analytical challenges	613:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	7	83	dep	gangliosides	1732:1743	arg1	GM3					1745:1747	GM3	1745:1747	GM3	1745:1747	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	83	dep	gangliosides	1732:1743	arg1	gangliosides					1732:1743	the simple gangliosides GM3 and GD3	1721:1755	the simple gangliosides GM3 and GD3	1721:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	83	dep	gangliosides	1732:1743	arg1	GD3					1753:1755	GD3	1753:1755	GD3	1753:1755	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	2	84	theme	due	606:608	arg1	hiPSC-CMs					596:604	hiPSC-CMs	596:604	hiPSC-CMs due to significant analytical challenges	596:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	8	85	theme	stem	1866:1869	arg1	cardiomyocytes					1884:1897	stem cell-derived cardiomyocytes	1866:1897	stem cell-derived cardiomyocytes	1866:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	1	86	theme	tissue	333:338	arg1	transplants					340:350	tissue transplants	333:350	tissue transplants in regenerative medicine	333:375	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	5	87	theme	migration	1090:1098	arg1	database					1105:1112	a migration time database	1088:1112	a migration time database comprising 38 different glycan species	1088:1151	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	2	88	theme	surface-exposed	509:523	arg1	biomolecules					525:536	highly diverse surface-exposed biomolecules	494:536	highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges	494:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	2	88	theme	surface-exposed	509:523	arg1	markers					584:590	potential surface markers	566:590	potential surface markers for hiPSC-CMs due to significant analytical challenges	566:645	Glycosphingolipids (GSLs) are a family of highly diverse surface-exposed biomolecules that have been neglected as potential surface markers for hiPSC-CMs due to significant analytical challenges.					
31058489	3	89	theme	GSL-derived	753:763	arg1	glycans					765:771	GSL-derived glycans	753:771	GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	753:969	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	0	90	theme	Glycosylation	44:56	arg1	Profiling					13:21	Profiling	13:21	Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis	13:101	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	0	91	theme	Multiplexed	61:71	arg1	Electrophoresis					87:101	Multiplexed Capillary Gel Electrophoresis	61:101	Multiplexed Capillary Gel Electrophoresis	61:101	Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection To Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes.					
31058489	6	92	theme	hiPSC-CMs	1451:1459	arg1	glycosylation					1384:1396	GSL glycosylation	1380:1396	GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs	1380:1459	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	3	93	theme	ceramide	782:789	arg1	glycanase					791:799	ceramide glycanase	782:799	ceramide glycanase digestion	782:809	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	7	94	theme	stage-specific	1651:1664	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	94	theme	stage-specific	1651:1664	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	5	95	theme	glycan	1138:1143	arg1	species					1145:1151	38 different glycan species	1125:1151	38 different glycan species	1125:1151	We built up a migration time database comprising 38 different glycan species, and we showed exemplarily that as few as 10 pg of fucosyl lactotetra was detectable.					
31058489	8	96	theme	first	1783:1787	arg1	This					1771:1774	This	1771:1774	This	1771:1774	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	8	96	theme	first	1783:1787	arg1	description					1789:1799	the first description	1779:1799	the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes	1779:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	1	97	theme	pluripotent	273:283	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	97	theme	pluripotent	273:283	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	8	98	link	cell-derived	1871:1882	arg1	cardiomyocytes					1884:1897	stem cell-derived cardiomyocytes	1866:1897	stem cell-derived cardiomyocytes	1866:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	7	99	theme	cell	1639:1642	arg1	antigen					1676:1682	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3	1601:1684	the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3)	1601:1692	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	7	99	theme	cell	1639:1642	arg1	SSEA3					1687:1691	SSEA3	1687:1691	SSEA3	1687:1691	In our study, we observed a general switch from complex GSLs with lacto- and globo-series core structures comprising the well-known human pluripotent stem cell marker stage-specific embryonic antigen 3 (SSEA3) and SSEA4 in hiPSCs toward the simple gangliosides GM3 and GD3 in hiPSC-CMs.					
31058489	6	100	theme	GSL	1239:1241	arg1	profiling					1250:1258	GSL glycan profiling	1239:1258	GSL glycan profiling	1239:1258	GSL glycan profiling could be performed with 105 human induced pluripotent stem cells, and we quantitatively dissected global alterations of GSL glycosylation of human induced pluripotent stem cells (hiPSCs) and hiPSC-CMs by employing xCGE-LIF.					
31058489	3	101	theme	8-aminopyrene-1,3,6-trisulfonic	812:842	arg1	APTS					850:853	APTS	850:853	APTS	850:853	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	3	101	theme	8-aminopyrene-1,3,6-trisulfonic	812:842	arg1	acid					844:847	8-aminopyrene-1,3,6-trisulfonic acid	812:847	8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling	812:863	Here, we describe the development of a novel and high-throughput-compatible workflow for the analysis of GSL-derived glycans based on ceramide glycanase digestion, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) labeling, and multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
31058489	8	102	from	GSLs	1838:1841	arg1	surface					1855:1861	the cell surface	1846:1861	the cell surface of stem cell-derived cardiomyocytes	1846:1897	This is the first description of GM3 and GD3 being highly abundant GSLs on the cell surface of stem cell-derived cardiomyocytes.					
31058489	1	103	theme	cell-derived	290:301	arg1	hiPSC-CMs					319:327	hiPSC-CMs	319:327	hiPSC-CMs	319:327	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
31058489	1	103	theme	cell-derived	290:301	arg1	cardiomyocytes					303:316	human induced pluripotent stem cell-derived cardiomyocytes	259:316	human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	259:328	Application of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as tissue transplants in regenerative medicine depends on cell-surface marker-based characterization and/or purification.					
29674057	8	0	theme	sample	1330:1335	arg1	volume					1347:1352	the sample injection volume	1326:1352	the sample injection volume	1326:1352	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	8	0	theme	sample	1330:1335	arg1	larger					1362:1367	larger	1362:1367	larger	1362:1367	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	12	1	theme	difluoroacetic	2023:2036	arg1	acid					2038:2041	difluoroacetic acid	2023:2041	difluoroacetic acid	2023:2041	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	9	2	theme	methionine-loss	1590:1604	arg1	products					1606:1613	N-terminal methionine-loss products	1579:1613	N-terminal methionine-loss products	1579:1613	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	11	3	with	Comparisons	1798:1808	arg1	selectivity					1831:1841	the selectivity	1827:1841	the selectivity provided by capillary electrophoresis-MS for the same glycoproteins	1827:1909	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	11	4	theme	same	1892:1895	arg1	glycoproteins					1897:1909	the same glycoproteins	1888:1909	the same glycoproteins	1888:1909	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	5	5	theme	pharmaceutical	779:792	arg1	proteins					794:801	intact pharmaceutical proteins	772:801	intact pharmaceutical proteins	772:801	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	4	6	gly	glycoforms	589:598	arg1	protein					581:587	protein glycoforms	581:598	protein glycoforms	581:598	Yet, resolving protein glycoforms at the intact level is challenging.					
29674057	11	7	gly	glycoproteins	1897:1909	arg1	glycoproteins					1897:1909	the same glycoproteins	1888:1909	the same glycoproteins	1888:1909	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	10	8	theme	marginal	1762:1769	arg1	effect					1771:1776	a marginal effect	1760:1776	a marginal effect	1760:1776	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	6	9	theme	glycoform	932:940	arg1	separation					942:951	Efficient glycoform separation	922:951	Efficient glycoform separation	922:951	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	2	10	theme	glycans	323:329	arg1	position					307:314	position	307:314	position	307:314	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	2	10	theme	glycans	323:329	arg1	number					296:301	number	296:301	number	296:301	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	2	10	theme	glycans	323:329	arg1	nature					288:293	nature	288:293	nature	288:293	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	12	11	theme	glycoform	2195:2203	arg1	coverage					2205:2212	the overall rhEPO glycoform coverage	2177:2212	the overall rhEPO glycoform coverage to 72	2177:2218	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	5	12	theme	glycosylation	746:758	arg1	patterns					760:767	glycosylation patterns	746:767	glycosylation patterns of intact pharmaceutical proteins	746:801	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	9	13	theme	rhIFN-β - 1a	1392:1403	arg1	HILIC-MS					1380:1387	HILIC-MS	1380:1387	HILIC-MS of rhIFN-β - 1a and rhEPO	1380:1413	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	7	14	theme	In-source	1075:1083	arg1	dissociation					1103:1114	In-source collision-induced dissociation	1075:1114	In-source collision-induced dissociation	1075:1114	In-source collision-induced dissociation proved to be very useful to minimize protein-signal suppression effects by TFA.					
29674057	2	15	dep	nature	288:293	arg1	the					284:286	the	284:286	the	284:286	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	9	16	theme	rhEPO	1409:1413	arg1	HILIC-MS					1380:1387	HILIC-MS	1380:1387	HILIC-MS of rhIFN-β - 1a and rhEPO	1380:1413	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	5	17	gly	glycosylation	746:758	arg1	proteins					794:801	intact pharmaceutical proteins	772:801	intact pharmaceutical proteins	772:801	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	12	18	theme	overall	2181:2187	arg1	coverage					2205:2212	the overall rhEPO glycoform coverage	2177:2212	the overall rhEPO glycoform coverage to 72	2177:2218	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	3	19	theme	glycans	361:367	arg1	analysis					340:347	analysis	340:347	analysis of released glycans and glycopeptides	340:385	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	5	20	gly	glycoproteins	828:840	arg1	glycoproteins					828:840	the complex glycoproteins	816:840	the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	816:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	20	gly	glycoproteins	828:840	arg1	interferon-beta-1a					842:859	interferon-beta-1a	842:859	interferon-beta-1a (rhIFN-β - 1a)	842:874	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	20	gly	glycoproteins	828:840	arg1	erythropoietin					898:911	recombinant human erythropoietin	880:911	recombinant human erythropoietin (rhEPO)	880:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	1	21	theme	critical	158:165	arg1	attribute					175:183	a critical quality attribute	156:183	a critical quality attribute of therapeutic proteins	156:207	Glycosylation is considered a critical quality attribute of therapeutic proteins.					
29674057	7	22	theme	collision-induced	1085:1101	arg1	dissociation					1103:1114	In-source collision-induced dissociation	1075:1114	In-source collision-induced dissociation	1075:1114	In-source collision-induced dissociation proved to be very useful to minimize protein-signal suppression effects by TFA.					
29674057	7	23	theme	protein-signal	1153:1166	arg1	effects					1180:1186	protein-signal suppression effects	1153:1186	protein-signal suppression effects	1153:1186	In-source collision-induced dissociation proved to be very useful to minimize protein-signal suppression effects by TFA.					
29674057	0	24	theme	hydrophilic	70:80	arg1	spectrometry					114:125	hydrophilic interaction chromatography-mass spectrometry	70:125	hydrophilic interaction chromatography-mass spectrometry	70:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	9	25	theme	15	1456:1457	arg1	compositions					1476:1487	15 and 51 glycoform compositions	1456:1487	compositions	1476:1487	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	1	26	theme	therapeutic	188:198	arg1	proteins					200:207	therapeutic proteins	188:207	therapeutic proteins	188:207	Glycosylation is considered a critical quality attribute of therapeutic proteins.					
29674057	3	27	theme	glycopeptides	373:385	arg1	analysis					340:347	analysis	340:347	analysis of released glycans and glycopeptides	340:385	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	0	28	theme	chromatography-mass	94:112	arg1	spectrometry					114:125	hydrophilic interaction chromatography-mass spectrometry	70:125	hydrophilic interaction chromatography-mass spectrometry	70:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	9	29	theme	51	1463:1464	arg1	compositions					1476:1487	15 and 51 glycoform compositions	1456:1487	compositions	1476:1487	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	8	30	theme	Direct	1196:1201	arg1	injection					1203:1211	Direct injection	1196:1211	Direct injection of therapeutic proteins in aqueous formulation	1196:1258	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	5	31	theme	chromatography-mass	688:706	arg1	spectrometry					708:719	hydrophilic liquid chromatography-mass spectrometry	669:719	hydrophilic liquid chromatography-mass spectrometry (HILIC-MS)	669:730	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	31	theme	chromatography-mass	688:706	arg1	HILIC-MS					722:729	HILIC-MS	722:729	HILIC-MS	722:729	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	9	32	theme	compositions	1476:1487	arg1	assignment					1427:1436	the assignment	1423:1436	the assignment of, respectively, 15 and 51 glycoform compositions	1423:1487	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	3	33	theme	individual	511:520	arg1	proteoforms					522:532	individual proteoforms	511:532	individual proteoforms	511:532	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	5	34	theme	recombinant	880:890	arg1	erythropoietin					898:911	recombinant human erythropoietin	880:911	recombinant human erythropoietin (rhEPO)	880:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	34	theme	recombinant	880:890	arg1	glycoproteins					828:840	the complex glycoproteins	816:840	the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	816:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	34	theme	recombinant	880:890	arg1	rhEPO					914:918	rhEPO	914:918	rhEPO	914:918	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	8	35	theme	proteins	1228:1235	arg1	injection					1203:1211	Direct injection	1196:1211	Direct injection of therapeutic proteins in aqueous formulation	1196:1258	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	3	36	theme	glycan	453:458	arg1	composition					418:428	composition	418:428	composition	418:428	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	10	37	theme	glycan	1657:1662	arg1	units					1664:1668	neutral glycan units	1649:1668	neutral glycan units	1649:1668	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	0	38	theme	interaction	82:92	arg1	spectrometry					114:125	hydrophilic interaction chromatography-mass spectrometry	70:125	hydrophilic interaction chromatography-mass spectrometry	70:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	0	39	theme	High-resolution	0:14	arg1	profiling					26:34	High-resolution glycoform profiling	0:34	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry	0:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	8	40	theme	aqueous	1240:1246	arg1	formulation					1248:1258	aqueous formulation	1240:1258	aqueous formulation	1240:1258	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	5	41	theme	hydrophilic	669:679	arg1	spectrometry					708:719	hydrophilic liquid chromatography-mass spectrometry	669:719	hydrophilic liquid chromatography-mass spectrometry (HILIC-MS)	669:730	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	41	theme	hydrophilic	669:679	arg1	HILIC-MS					722:729	HILIC-MS	722:729	HILIC-MS	722:729	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	3	42	theme	modifications	551:563	arg1	assignment					497:506	assignment	497:506	assignment of individual proteoforms and co-occurring modifications	497:563	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	6	43	theme	trifluoroacetic	1028:1042	arg1	TFA					1050:1052	TFA	1050:1052	TFA	1050:1052	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	6	43	theme	trifluoroacetic	1028:1042	arg1	acid					1044:1047	trifluoroacetic acid	1028:1047	trifluoroacetic acid (TFA)	1028:1053	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	11	44	theme	remarkable	1923:1932	arg1	complementarity					1934:1948	a remarkable complementarity	1921:1948	a remarkable complementarity of the techniques	1921:1966	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	0	45	theme	intact	39:44	arg1	proteins					58:65	intact therapeutic proteins	39:65	intact therapeutic proteins	39:65	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	9	46	theme	modifications	1529:1541	arg1	oxidation					1565:1573	oxidation	1565:1573	oxidation	1565:1573	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	46	theme	modifications	1529:1541	arg1	succinimide					1552:1562	succinimide	1552:1562	succinimide	1552:1562	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	46	theme	modifications	1529:1541	arg1	products					1606:1613	N-terminal methionine-loss products	1579:1613	N-terminal methionine-loss products	1579:1613	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	46	theme	modifications	1529:1541	arg1	variety					1500:1506	a variety	1498:1506	a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products	1498:1613	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	46	theme	modifications	1529:1541	arg1	modifications					1529:1541	posttranslational modifications	1511:1541	posttranslational modifications	1511:1541	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	12	47	theme	relative	2106:2113	arg1	abundances					2115:2124	relative abundances	2106:2124	relative abundances below 1%	2106:2133	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	12	48	theme	peak	2044:2047	arg1	resolution					2049:2058	peak resolution	2044:2058	peak resolution	2044:2058	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	3	49	theme	intact	461:466	arg1	analysis					481:488	intact glycoprotein analysis	461:488	intact glycoprotein analysis	461:488	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	10	50	theme	sialic	1738:1743	arg1	acids					1745:1749	terminal sialic acids	1729:1749	terminal sialic acids	1729:1749	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	2	51	theme	Protein	210:216	arg1	heterogeneity					218:230	Protein heterogeneity	210:230	Protein heterogeneity introduced by glycosylation	210:258	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	0	52	theme	proteins	58:65	arg1	profiling					26:34	High-resolution glycoform profiling	0:34	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry	0:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	8	53	theme	extra	1289:1293	arg1	dispersion					1300:1309	extra band dispersion	1289:1309	extra band dispersion	1289:1309	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	6	54	theme	HILIC	1001:1005	arg1	phase					1018:1022	a superficially-porous amide HILIC stationary phase	972:1022	a superficially-porous amide HILIC stationary phase	972:1022	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	11	55	theme	techniques	1957:1966	arg1	complementarity					1934:1948	a remarkable complementarity	1921:1948	a remarkable complementarity of the techniques	1921:1966	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	3	56	gly	glycopeptides	373:385	arg2	glycopeptides					373:385	glycopeptides	373:385	glycopeptides	373:385	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	6	57	theme	superficially-porous	974:993	arg1	phase					1018:1022	a superficially-porous amide HILIC stationary phase	972:1022	a superficially-porous amide HILIC stationary phase	972:1022	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	9	58	theme	N-terminal	1579:1588	arg1	products					1606:1613	N-terminal methionine-loss products	1579:1613	N-terminal methionine-loss products	1579:1613	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	10	59	theme	HILIC	1781:1785	arg1	retention					1787:1795	HILIC retention	1781:1795	HILIC retention	1781:1795	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	5	60	theme	intact	772:777	arg1	proteins					794:801	intact pharmaceutical proteins	772:801	intact pharmaceutical proteins	772:801	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	8	61	theme	injection	1337:1345	arg1	volume					1347:1352	the sample injection volume	1326:1352	the sample injection volume	1326:1352	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	8	61	theme	injection	1337:1345	arg1	larger					1362:1367	larger	1362:1367	larger	1362:1367	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	2	62	from	differences	269:279	arg1	position					307:314	position	307:314	position	307:314	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	2	62	from	differences	269:279	arg1	number					296:301	number	296:301	number	296:301	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	2	62	from	differences	269:279	arg1	nature					288:293	nature	288:293	nature	288:293	Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position of the glycans.					
29674057	6	63	theme	Efficient	922:930	arg1	separation					942:951	Efficient glycoform separation	922:951	Efficient glycoform separation	922:951	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	3	64	dep	composition	418:428	arg1	the					414:416	the	414:416	the	414:416	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	12	65	theme	rhEPO	2189:2193	arg1	coverage					2205:2212	the overall rhEPO glycoform coverage	2177:2212	the overall rhEPO glycoform coverage to 72	2177:2218	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	5	66	theme	complex	820:826	arg1	glycoproteins					828:840	the complex glycoproteins	816:840	the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	816:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	66	theme	complex	820:826	arg1	interferon-beta-1a					842:859	interferon-beta-1a	842:859	interferon-beta-1a (rhIFN-β - 1a)	842:874	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	66	theme	complex	820:826	arg1	erythropoietin					898:911	recombinant human erythropoietin	880:911	recombinant human erythropoietin (rhEPO)	880:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	8	67	from	injection	1203:1211	arg1	formulation					1248:1258	aqueous formulation	1240:1258	aqueous formulation	1240:1258	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	8	68	dep	possible	1264:1271	arg1	provided					1312:1319	provided	1312:1319	provided that the sample injection volume was not larger than 2 μL	1312:1377	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	5	69	theme	proteins	794:801	arg1	patterns					760:767	glycosylation patterns	746:767	glycosylation patterns of intact pharmaceutical proteins	746:801	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	8	70	theme	therapeutic	1216:1226	arg1	proteins					1228:1235	therapeutic proteins	1216:1235	therapeutic proteins	1216:1235	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	1	71	theme	quality	167:173	arg1	attribute					175:183	a critical quality attribute	156:183	a critical quality attribute of therapeutic proteins	156:207	Glycosylation is considered a critical quality attribute of therapeutic proteins.					
29674057	3	72	theme	released	352:359	arg1	glycans					361:367	released glycans	352:367	released glycans	352:367	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	5	73	theme	human	892:896	arg1	erythropoietin					898:911	recombinant human erythropoietin	880:911	recombinant human erythropoietin (rhEPO)	880:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	73	theme	human	892:896	arg1	glycoproteins					828:840	the complex glycoproteins	816:840	the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	816:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	73	theme	human	892:896	arg1	rhEPO					914:918	rhEPO	914:918	rhEPO	914:918	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	7	74	theme	suppression	1168:1178	arg1	effects					1180:1186	protein-signal suppression effects	1153:1186	protein-signal suppression effects	1153:1186	In-source collision-induced dissociation proved to be very useful to minimize protein-signal suppression effects by TFA.					
29674057	1	75	theme	proteins	200:207	arg1	attribute					175:183	a critical quality attribute	156:183	a critical quality attribute of therapeutic proteins	156:207	Glycosylation is considered a critical quality attribute of therapeutic proteins.					
29674057	5	76	dep	glycoproteins	828:840	arg1	erythropoietin					898:911	recombinant human erythropoietin	880:911	recombinant human erythropoietin (rhEPO)	880:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	76	dep	glycoproteins	828:840	arg1	rhIFN-β - 1a					862:873	rhIFN-β - 1a	862:873	rhIFN-β - 1a	862:873	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	76	dep	glycoproteins	828:840	arg1	interferon-beta-1a					842:859	interferon-beta-1a	842:859	interferon-beta-1a (rhIFN-β - 1a)	842:874	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	76	dep	glycoproteins	828:840	arg1	glycoproteins					828:840	the complex glycoproteins	816:840	the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	816:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	76	dep	glycoproteins	828:840	arg1	rhEPO					914:918	rhEPO	914:918	rhEPO	914:918	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	4	77	theme	intact	607:612	arg1	level					614:618	the intact level	603:618	the intact level	603:618	Yet, resolving protein glycoforms at the intact level is challenging.					
29674057	9	78	theme	glycoform	1466:1474	arg1	compositions					1476:1487	15 and 51 glycoform compositions	1456:1487	compositions	1476:1487	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	3	79	gly	glycoprotein	468:479	arg1	glycoprotein					468:479	intact glycoprotein analysis	461:488	intact glycoprotein analysis	461:488	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	5	80	theme	spectrometry	708:719	arg1	capacity					657:664	the capacity	653:664	the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO)	653:919	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	10	81	theme	MS-based	1616:1623	arg1	assignments					1625:1635	MS-based assignments	1616:1635	MS-based assignments	1616:1635	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	12	82	gly	glycoforms	2090:2099	arg1	rhEPO					2084:2088	rhEPO glycoforms	2084:2099	rhEPO glycoforms with relative abundances below 1%	2084:2133	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	10	83	contain	had	1756:1758	arg1	acids					1745:1749	terminal sialic acids	1729:1749	terminal sialic acids	1729:1749	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	10	83	contain	had	1756:1758	arg2	effect					1771:1776	a marginal effect	1760:1776	a marginal effect	1760:1776	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	4	84	theme	protein	581:587	arg1	glycoforms					589:598	protein glycoforms	581:598	protein glycoforms	581:598	Yet, resolving protein glycoforms at the intact level is challenging.					
29674057	3	85	theme	proteoforms	522:532	arg1	assignment					497:506	assignment	497:506	assignment of individual proteoforms and co-occurring modifications	497:563	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	0	86	theme	glycoform	16:24	arg1	profiling					26:34	High-resolution glycoform profiling	0:34	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry	0:125	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	10	87	theme	neutral	1649:1655	arg1	units					1664:1668	neutral glycan units	1649:1668	neutral glycan units	1649:1668	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	5	88	theme	liquid	681:686	arg1	spectrometry					708:719	hydrophilic liquid chromatography-mass spectrometry	669:719	hydrophilic liquid chromatography-mass spectrometry (HILIC-MS)	669:730	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	5	88	theme	liquid	681:686	arg1	HILIC-MS					722:729	HILIC-MS	722:729	HILIC-MS	722:729	We have explored the capacity of hydrophilic liquid chromatography-mass spectrometry (HILIC-MS) for assessing glycosylation patterns of intact pharmaceutical proteins by analyzing the complex glycoproteins interferon-beta-1a (rhIFN-β - 1a) and recombinant human erythropoietin (rhEPO).					
29674057	10	89	theme	glycoform	1699:1707	arg1	separation					1709:1718	glycoform separation	1699:1718	glycoform separation	1699:1718	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	0	90	theme	therapeutic	46:56	arg1	proteins					58:65	intact therapeutic proteins	39:65	intact therapeutic proteins	39:65	High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.					
29674057	9	91	theme	posttranslational	1511:1527	arg1	oxidation					1565:1573	oxidation	1565:1573	oxidation	1565:1573	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	91	theme	posttranslational	1511:1527	arg1	succinimide					1552:1562	succinimide	1552:1562	succinimide	1552:1562	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	91	theme	posttranslational	1511:1527	arg1	products					1606:1613	N-terminal methionine-loss products	1579:1613	N-terminal methionine-loss products	1579:1613	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	9	91	theme	posttranslational	1511:1527	arg1	modifications					1529:1541	posttranslational modifications	1511:1541	posttranslational modifications	1511:1541	HILIC-MS of rhIFN-β - 1a and rhEPO allowed the assignment of, respectively, 15 and 51 glycoform compositions, next to a variety of posttranslational modifications, such as succinimide, oxidation and N-terminal methionine-loss products.					
29674057	3	92	theme	co-occurring	538:549	arg1	modifications					551:563	co-occurring modifications	538:563	co-occurring modifications	538:563	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	8	93	theme	band	1295:1298	arg1	dispersion					1300:1309	extra band dispersion	1289:1309	extra band dispersion	1289:1309	Direct injection of therapeutic proteins in aqueous formulation was possible without causing extra band dispersion, provided that the sample injection volume was not larger than 2 μL.					
29674057	6	94	theme	stationary	1007:1016	arg1	phase					1018:1022	a superficially-porous amide HILIC stationary phase	972:1022	a superficially-porous amide HILIC stationary phase	972:1022	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	3	95	theme	glycoprotein	468:479	arg1	analysis					481:488	intact glycoprotein analysis	461:488	intact glycoprotein analysis	461:488	Whereas analysis of released glycans and glycopeptides provides information about the composition and/or position of the glycan, intact glycoprotein analysis allows assignment of individual proteoforms and co-occurring modifications.					
29674057	10	96	theme	terminal	1729:1736	arg1	acids					1745:1749	terminal sialic acids	1729:1749	terminal sialic acids	1729:1749	MS-based assignments showed that neutral glycan units significantly contributed to glycoform separation, whereas terminal sialic acids only had a marginal effect on HILIC retention.					
29674057	12	97	theme	rhEPO	2084:2088	arg1	glycoforms					2090:2099	rhEPO glycoforms	2084:2099	rhEPO glycoforms with relative abundances below 1%	2084:2133	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
29674057	6	98	theme	amide	995:999	arg1	phase					1018:1022	a superficially-porous amide HILIC stationary phase	972:1022	a superficially-porous amide HILIC stationary phase	972:1022	Efficient glycoform separation was achieved using a superficially-porous amide HILIC stationary phase and trifluoroacetic acid (TFA) as eluent additive.					
29674057	11	99	theme	capillary	1855:1863	arg1	electrophoresis-MS					1865:1882	capillary electrophoresis-MS	1855:1882	capillary electrophoresis-MS for the same glycoproteins	1855:1909	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	11	100	theme	HILIC-MS	1813:1820	arg1	Comparisons					1798:1808	Comparisons	1798:1808	Comparisons	1798:1808	Comparisons of HILIC-MS with the selectivity provided by capillary electrophoresis-MS for the same glycoproteins, revealed a remarkable complementarity of the techniques.					
29674057	12	101	with	glycoforms	2090:2099	arg1	abundances					2115:2124	relative abundances	2106:2124	relative abundances below 1%	2106:2133	Finally it was demonstrated that by replacing TFA for difluoroacetic acid, peak resolution somewhat decreased, but rhEPO glycoforms with relative abundances below 1% could be detected by HILIC-MS, increasing the overall rhEPO glycoform coverage to 72.					
31285016	7	0	theme	lees	1512:1515	arg1	management					1492:1501	the management	1488:1501	the management of gross lees in a winery environment	1488:1539	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	7	1	theme	winemaking	1365:1374	arg1	techniques					1376:1385	winemaking techniques	1365:1385	winemaking techniques	1365:1385	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	3	2	from	origin	519:524	arg1	material					539:546	the grape material	529:546	the grape material	529:546	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	3	2	from	origin	519:524	arg1	polysaccharides					492:506	the polysaccharides	488:506	the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process	488:605	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	7	3	from	environment	1529:1539	arg1	management					1492:1501	the management	1488:1501	the management of gross lees in a winery environment	1488:1539	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	4	4	theme	sediment	863:870	arg1	compositions					872:883	wine sediment compositions	858:883	wine sediment compositions	858:883	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	2	5	theme	biochemical	282:292	arg1	composition					294:304	the biochemical composition	278:304	the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix	278:415	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	5	6	theme	extensins	1089:1097	arg1	abundance					1076:1084	an abundance	1073:1084	an abundance of extensins and arabinogalactan proteins	1073:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	4	7	theme	grapes	747:752	arg1	composition					713:723	the glycan-rich composition	697:723	the glycan-rich composition of particularly white grapes	697:752	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	3	8	theme	rapid	467:471	arg1	means					473:477	a relatively rapid means	454:477	a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process	454:605	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	4	9	theme	formulations	842:853	arg1	effects					797:803	the effects	793:803	the effects of recombinant and commercial enzyme formulations on wine sediment compositions	793:883	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	6	10	dep	homogalacturonan	1180:1195	arg1	off					1172:1174	off	1172:1174	off	1172:1174	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	6	10	dep	homogalacturonan	1180:1195	arg1	the					1176:1178	the	1176:1178	the	1176:1178	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	4	11	theme	Polymer	647:653	arg1	CoMPP					666:670	CoMPP	666:670	CoMPP	666:670	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	11	theme	Polymer	647:653	arg1	Profiling					655:663	Comprehensive Microarray Polymer Profiling	622:663	Comprehensive Microarray Polymer Profiling (CoMPP)	622:671	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	5	12	theme	arabinogalactan	1103:1117	arg1	proteins					1119:1126	arabinogalactan proteins	1103:1126	arabinogalactan proteins	1103:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	4	13	theme	enzyme	835:840	arg1	formulations					842:853	recombinant and commercial enzyme formulations	808:853	recombinant and commercial enzyme formulations	808:853	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	7	14	theme	sediment	1439:1446	arg1	compositions					1448:1459	the glycan-rich wine sediment compositions	1418:1459	the glycan-rich wine sediment compositions	1418:1459	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	3	15	theme	Glycan	418:423	arg1	technology					436:445	Glycan microarray technology	418:445	Glycan microarray technology	418:445	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	5	16	theme	proteins	1119:1126	arg1	abundance					1076:1084	an abundance	1073:1084	an abundance of extensins and arabinogalactan proteins	1073:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	4	17	theme	wine	858:861	arg1	compositions					872:883	wine sediment compositions	858:883	wine sediment compositions	858:883	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	18	used	used	677:680	arg2	CoMPP					666:670	CoMPP	666:670	CoMPP	666:670	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	18	used	used	677:680	arg2	Profiling					655:663	Comprehensive Microarray Polymer Profiling	622:663	Comprehensive Microarray Polymer Profiling (CoMPP)	622:671	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	2	19	theme	sediments	315:323	arg1	composition					294:304	the biochemical composition	278:304	the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix	278:415	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	7	20	theme	winery	1522:1527	arg1	environment					1529:1539	a winery environment	1520:1539	a winery environment	1520:1539	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	0	21	theme	Tracking	0:7	arg1	polysaccharides					9:23	Tracking polysaccharides	0:23	Tracking polysaccharides during white winemaking using glycan microarrays	0:72	Tracking polysaccharides during white winemaking using glycan microarrays reveals glycoprotein-rich sediments.					
31285016	6	22	theme	sediment	1265:1272	arg1	layer					1274:1278	a sediment layer	1263:1278	a sediment layer composed almost exclusively of extensins and arabinogalactan proteins	1263:1348	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	5	23	theme	enzymes	941:947	arg1	absence					930:936	the absence	926:936	the absence of enzymes	926:947	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	0	24	theme	white	32:36	arg1	winemaking					38:47	white winemaking	32:47	white winemaking using glycan microarrays	32:72	Tracking polysaccharides during white winemaking using glycan microarrays reveals glycoprotein-rich sediments.					
31285016	7	25	theme	glycan-rich	1422:1432	arg1	compositions					1448:1459	the glycan-rich wine sediment compositions	1418:1459	the glycan-rich wine sediment compositions	1418:1459	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	5	26	theme	arabinans	1034:1042	arg1	abundance					981:989	an abundance	978:989	an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins	978:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	3	27	theme	grape	533:537	arg1	material					539:546	the grape material	529:546	the grape material	529:546	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	6	28	theme	side	1241:1244	arg1	chains					1246:1251	its side chains	1237:1251	its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins	1237:1348	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	2	29	theme	grape	404:408	arg1	matrix					410:415	the grape matrix	400:415	the grape matrix	400:415	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	7	30	theme	wine	1434:1437	arg1	compositions					1448:1459	the glycan-rich wine sediment compositions	1418:1459	the glycan-rich wine sediment compositions	1418:1459	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	6	31	theme	enzymes	1145:1151	arg1	addition					1133:1140	The addition	1129:1140	The addition of enzymes	1129:1151	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	3	32	from	steps	575:579	arg1	process					599:605	the winemaking process	584:605	the winemaking process	584:605	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	7	33	from	management	1492:1501	arg1	environment					1529:1539	a winery environment	1520:1539	a winery environment	1520:1539	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	4	34	theme	Comprehensive	622:634	arg1	CoMPP					666:670	CoMPP	666:670	CoMPP	666:670	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	34	theme	Comprehensive	622:634	arg1	Profiling					655:663	Comprehensive Microarray Polymer Profiling	622:663	Comprehensive Microarray Polymer Profiling (CoMPP)	622:671	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	35	theme	recombinant	808:818	arg1	formulations					842:853	recombinant and commercial enzyme formulations	808:853	recombinant and commercial enzyme formulations	808:853	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	3	36	theme	microarray	425:434	arg1	technology					436:445	Glycan microarray technology	418:445	Glycan microarray technology	418:445	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	5	37	theme	rhamnogalacturonans	1013:1031	arg1	abundance					981:989	an abundance	978:989	an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins	978:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	2	38	theme	large	384:388	arg1	part					390:393	large part	384:393	large part	384:393	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	6	39	theme	arabinogalactan	1325:1339	arg1	proteins					1341:1348	arabinogalactan proteins	1325:1348	arabinogalactan proteins	1325:1348	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	4	40	theme	commercial	824:833	arg1	formulations					842:853	recombinant and commercial enzyme formulations	808:853	recombinant and commercial enzyme formulations	808:853	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	1	41	theme	significant	135:145	arg1	sediments					157:165	sediments	157:165	sediments that are formed in the tanks, the vats and in the bottles before and after fermentation	157:253	Winemaking results in a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation.					
31285016	1	41	theme	significant	135:145	arg1	amount					147:152	a significant amount	133:152	a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation	133:253	Winemaking results in a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation.					
31285016	0	42	theme	glycan	55:60	arg1	microarrays					62:72	glycan microarrays	55:72	glycan microarrays	55:72	Tracking polysaccharides during white winemaking using glycan microarrays reveals glycoprotein-rich sediments.					
31285016	2	43	attach	derived	373:379	arg2	they					350:353	they	350:353	they	350:353	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	2	43	attach	derived	373:379	arg1	matrix					410:415	the grape matrix	400:415	the grape matrix	400:415	Little is known about the biochemical composition of these sediments apart from the fact that they are assumed to be derived in large part from the grape matrix.					
31285016	3	44	theme	various	567:573	arg1	steps					575:579	the various steps	563:579	the various steps in the winemaking process	563:605	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
31285016	6	45	theme	rhamnogalacturonan	1213:1230	arg1	homogalacturonan					1180:1195	homogalacturonan	1180:1195	homogalacturonan	1180:1195	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	5	46	theme	homogalacturonans	994:1010	arg1	abundance					981:989	an abundance	978:989	an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins	978:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	5	47	theme	galactans	1048:1056	arg1	abundance					981:989	an abundance	978:989	an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins	978:1126	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	7	48	theme	techniques	1376:1385	arg1	effect					1355:1360	The effect	1351:1360	The effect of winemaking techniques	1351:1385	The effect of winemaking techniques was shown to have an effect on the glycan-rich wine sediment compositions and holds implications for the management of gross lees in a winery environment.					
31285016	4	49	theme	glycan-rich	701:711	arg1	composition					713:723	the glycan-rich composition	697:723	the glycan-rich composition of particularly white grapes	697:752	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	50	theme	white	741:745	arg1	grapes					747:752	particularly white grapes	728:752	particularly white grapes	728:752	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	5	51	dep	abundance	1076:1084	arg1	addition					1061:1068	addition	1061:1068	addition	1061:1068	The gross lees or sediments produced in the absence of enzymes were found to be composed of an abundance of homogalacturonans, rhamnogalacturonans, arabinans and galactans in addition to an abundance of extensins and arabinogalactan proteins.					
31285016	4	52	from	effects	797:803	arg1	compositions					872:883	wine sediment compositions	858:883	wine sediment compositions	858:883	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	1	53	theme	sediments	157:165	arg1	sediments					157:165	sediments	157:165	sediments that are formed in the tanks, the vats and in the bottles before and after fermentation	157:253	Winemaking results in a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation.					
31285016	1	53	theme	sediments	157:165	arg1	amount					147:152	a significant amount	133:152	a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation	133:253	Winemaking results in a significant amount of sediments that are formed in the tanks, the vats and in the bottles before and after fermentation.					
31285016	6	54	with	rhamnogalacturonan	1213:1230	arg1	chains					1246:1251	its side chains	1237:1251	its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins	1237:1348	The addition of enzymes was shown to strip off the homogalacturonan and much of the rhamnogalacturonan with its side chains revealing a sediment layer composed almost exclusively of extensins and arabinogalactan proteins.					
31285016	0	55	theme	glycoprotein-rich	82:98	arg1	sediments					100:108	glycoprotein-rich sediments	82:108	glycoprotein-rich sediments	82:108	Tracking polysaccharides during white winemaking using glycan microarrays reveals glycoprotein-rich sediments.					
31285016	4	56	theme	Microarray	636:645	arg1	CoMPP					666:670	CoMPP	666:670	CoMPP	666:670	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	4	56	theme	Microarray	636:645	arg1	Profiling					655:663	Comprehensive Microarray Polymer Profiling	622:663	Comprehensive Microarray Polymer Profiling (CoMPP)	622:671	In this study Comprehensive Microarray Polymer Profiling (CoMPP) was used to investigate the glycan-rich composition of particularly white grapes during winemaking and then investigate the effects of recombinant and commercial enzyme formulations on wine sediment compositions.					
31285016	3	57	theme	winemaking	588:597	arg1	process					599:605	the winemaking process	584:605	the winemaking process	584:605	Glycan microarray technology offers a relatively rapid means to track the polysaccharides from their origin in the grape material and throughout the various steps in the winemaking process.					
29975661	5	0	theme	pro-KLK	701:707	arg1	zymogens					709:716	soluble pro-KLK zymogens	693:716	soluble pro-KLK zymogens	693:716	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	4	1	theme	secretory	619:627	arg1	expression					629:638	secretory expression	619:638	secretory expression	619:638	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	1	2	theme	KLKs	178:181	arg1	group					165:169	the prostatic group	151:169	the prostatic group	151:169	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	2	theme	KLKs	178:181	arg1	KLKs					178:181	the KLKs	174:181	the KLKs	174:181	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	5	3	theme	zymogens	709:716	arg1	purification					677:688	the subsequent purification	662:688	the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms	662:765	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	7	4	theme	pro-KLKs	925:932	arg1	Activation					898:907	Activation	898:907	Activation of glycosylated pro-KLKs 4 and 11	898:941	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	8	5	with	KLKs	1060:1063	arg1	counterparts					1093:1104	their non-glycosylated counterparts	1070:1104	their non-glycosylated counterparts	1070:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	7	6	gly	glycosylated	912:923	arg1	pro-KLKs					925:932	glycosylated pro-KLKs 4 and 11	912:941	glycosylated pro-KLKs 4 and 11	912:941	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	3	7	theme	proteases	480:488	arg1	characterization					454:469	a detailed characterization	443:469	a detailed characterization of these proteases	443:488	However, this potential awaits a detailed characterization of these proteases.					
29975661	1	8	theme	major	190:194	arg1	function					210:217	function	210:217	function	210:217	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	5	9	theme	subsequent	666:675	arg1	purification					677:688	the subsequent purification	662:688	the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms	662:765	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	6	10	theme	mass	837:840	arg1	spectrometry					842:853	mass spectrometry	837:853	mass spectrometry	837:853	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	7	11	theme	glycosylated	912:923	arg1	pro-KLKs					925:932	glycosylated pro-KLKs 4 and 11	912:941	glycosylated pro-KLKs 4 and 11	912:941	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	9	12	theme	biology	1391:1397	arg1	understanding					1368:1380	a deeper understanding	1359:1380	a deeper understanding of their biology	1359:1397	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	8	13	theme	activation	1209:1218	arg1	profiles					1220:1227	their activation profiles	1203:1227	their activation profiles	1203:1227	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	5	14	theme	correct	723:729	arg1	propeptides					731:741	correct propeptides	723:741	correct propeptides	723:741	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	4	15	theme	prostatic	522:530	arg1	KLKs					532:535	glycosylated prostatic KLKs	509:535	glycosylated prostatic KLKs resembling the natural proteases	509:568	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	9	16	theme	functional	1278:1287	arg1	role					1289:1292	The functional role	1274:1292	The functional role of glycosylation in prostate-specific KLKs	1274:1335	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	4	17	theme	glycosylated	509:520	arg1	KLKs					532:535	glycosylated prostatic KLKs	509:535	glycosylated prostatic KLKs resembling the natural proteases	509:568	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	9	18	theme	prostate-specific	1314:1330	arg1	KLKs					1332:1335	prostate-specific KLKs	1314:1335	prostate-specific KLKs	1314:1335	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	4	19	gly	glycosylated	509:520	arg1	KLKs					532:535	glycosylated prostatic KLKs	509:535	glycosylated prostatic KLKs resembling the natural proteases	509:568	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	1	20	theme	Human	58:62	arg1	peptidases					83:92	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	20	theme	Human	58:62	arg1	activator					118:126	the activator	114:126	the activator of KLK3/PSA	114:138	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	8	21	gly	glycosylated	1037:1048	arg1	KLKs					1060:1063	the glycosylated prostatic KLKs	1033:1063	the glycosylated prostatic KLKs with their non-glycosylated counterparts	1033:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	2	22	theme	prostate	314:321	arg1	cancer					323:328	prostate cancer	314:328	prostate cancer	314:328	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	9	23	from	role	1289:1292	arg1	KLKs					1332:1335	prostate-specific KLKs	1314:1335	prostate-specific KLKs	1314:1335	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	1	24	theme	physiological	196:208	arg1	function					210:217	function	210:217	function	210:217	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	25	theme	kallikrein-related	64:81	arg1	peptidases					83:92	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	25	theme	kallikrein-related	64:81	arg1	activator					118:126	the activator	114:126	the activator of KLK3/PSA	114:138	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	26	theme	KLK3/PSA	131:138	arg1	peptidases					83:92	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2	58:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	26	theme	KLK3/PSA	131:138	arg1	activator					118:126	the activator	114:126	the activator of KLK3/PSA	114:138	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	5	27	theme	mature	754:759	arg1	forms					761:765	the mature forms	750:765	the mature forms	750:765	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	9	28	theme	glycosylation	1297:1309	arg1	role					1289:1292	The functional role	1274:1292	The functional role of glycosylation in prostate-specific KLKs	1274:1335	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	2	29	theme	clinical	346:353	arg1	KLKs					290:293	these KLKs	284:293	these KLKs	284:293	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	2	29	theme	clinical	346:353	arg1	biomarkers					355:364	clinical biomarkers	346:364	clinical biomarkers	346:364	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	5	30	theme	forms	761:765	arg1	purification					677:688	the subsequent purification	662:688	the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms	662:765	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	1	31	theme	semen	222:226	arg1	liquefaction					228:239	semen liquefaction	222:239	semen liquefaction	222:239	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	8	32	theme	glycosylated	1037:1048	arg1	KLKs					1060:1063	the glycosylated prostatic KLKs	1033:1063	the glycosylated prostatic KLKs with their non-glycosylated counterparts	1033:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	6	33	theme	deglycosylation	809:823	arg1	assays					825:830	enzymatic deglycosylation assays	799:830	enzymatic deglycosylation assays	799:830	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	4	34	theme	Leishmania	579:588	arg1	LEXSY					591:595	Leishmania (LEXSY) and HEK293 cells	579:613	LEXSY	591:595	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	0	35	theme	glycosylated	27:38	arg1	activity					15:22	activity	15:22	activity	15:22	Activation and activity of glycosylated KLKs 3, 4 and 11.					
29975661	0	35	theme	glycosylated	27:38	arg1	Activation					0:9	Activation	0:9	Activation	0:9	Activation and activity of glycosylated KLKs 3, 4 and 11.					
29975661	2	36	theme	therapeutic	391:401	arg1	KLKs					290:293	these KLKs	284:293	these KLKs	284:293	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	2	36	theme	therapeutic	391:401	arg1	targets					403:409	therapeutic targets	391:409	therapeutic targets	391:409	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	6	37	theme	enzymatic	799:807	arg1	assays					825:830	enzymatic deglycosylation assays	799:830	enzymatic deglycosylation assays	799:830	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	5	38	with	purification	677:688	arg1	propeptides					731:741	correct propeptides	723:741	correct propeptides	723:741	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	8	39	gly	non-glycosylated	1076:1091	arg1	counterparts					1093:1104	their non-glycosylated counterparts	1070:1104	their non-glycosylated counterparts	1070:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	8	40	theme	non-glycosylated	1076:1091	arg1	counterparts					1093:1104	their non-glycosylated counterparts	1070:1104	their non-glycosylated counterparts	1070:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	2	41	used	used	338:341	arg2	targets					403:409	therapeutic targets	391:409	therapeutic targets	391:409	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	2	41	used	used	338:341	arg2	biomarkers					355:364	clinical biomarkers	346:364	clinical biomarkers	346:364	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	2	41	used	used	338:341	arg2	KLKs					290:293	these KLKs	284:293	these KLKs	284:293	Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets.					
29975661	8	42	theme	KLK	1178:1180	arg1	proteases					1182:1190	the KLK proteases	1174:1190	the KLK proteases	1174:1190	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	6	43	theme	KLKs	892:895	arg1	glycosylation					869:881	the glycosylation	865:881	the glycosylation of these KLKs	865:895	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	8	44	theme	prostatic	1050:1058	arg1	KLKs					1060:1063	the glycosylated prostatic KLKs	1033:1063	the glycosylated prostatic KLKs with their non-glycosylated counterparts	1033:1104	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	1	45	theme	fertilization	252:264	arg1	process					266:272	the fertilization process	248:272	the fertilization process	248:272	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	8	46	theme	enzymatic	1239:1247	arg1	activity					1249:1256	their enzymatic activity	1233:1256	their enzymatic activity to some extent	1233:1271	By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent.					
29975661	6	47	theme	acid-Schiff	777:787	arg1	reaction					789:796	Periodic acid-Schiff reaction	768:796	Periodic acid-Schiff reaction	768:796	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	7	48	gly	glycosylated	978:989	arg1	KLK2					991:994	glycosylated KLK2	978:994	glycosylated KLK2	978:994	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	1	49	theme	prostatic	155:163	arg1	group					165:169	the prostatic group	151:169	the prostatic group	151:169	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	49	theme	prostatic	155:163	arg1	KLKs					178:181	the KLKs	174:181	the KLKs	174:181	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	7	50	theme	glycosylated	978:989	arg1	KLK2					991:994	glycosylated KLK2	978:994	glycosylated KLK2	978:994	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	6	51	theme	Periodic	768:775	arg1	reaction					789:796	Periodic acid-Schiff reaction	768:796	Periodic acid-Schiff reaction	768:796	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	4	52	used	used	574:577	arg2	we					571:572	we	571:572	we	571:572	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	3	53	theme	detailed	445:452	arg1	characterization					454:469	a detailed characterization	443:469	a detailed characterization of these proteases	443:488	However, this potential awaits a detailed characterization of these proteases.					
29975661	7	54	dep	pro-KLKs	925:932	arg1	11					940:941	11	940:941	11	940:941	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	7	54	dep	pro-KLKs	925:932	arg1	4					934:934	4	934:934	4	934:934	Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively.					
29975661	1	55	dep	peptidases	83:92	arg1	11					100:101	11	100:101	11	100:101	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	55	dep	peptidases	83:92	arg1	KLK2					108:111	KLK2	108:111	KLK2	108:111	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	55	dep	peptidases	83:92	arg1	4					97:97	4	97:97	4	97:97	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	1	55	dep	peptidases	83:92	arg1	3					94:94	3	94:94	3	94:94	Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process.					
29975661	5	56	theme	soluble	693:699	arg1	zymogens					709:716	soluble pro-KLK zymogens	693:716	soluble pro-KLK zymogens	693:716	Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms.					
29975661	9	57	theme	medical	1406:1412	arg1	applications					1414:1425	medical applications	1406:1425	medical applications	1406:1425	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	9	58	theme	deeper	1361:1366	arg1	understanding					1368:1380	a deeper understanding	1359:1380	a deeper understanding of their biology	1359:1397	The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.					
29975661	4	59	theme	HEK293	602:607	arg1	cells					609:613	Leishmania (LEXSY) and HEK293 cells	579:613	cells	609:613	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29975661	6	60	gly	glycosylation	869:881	arg1	KLKs					892:895	these KLKs	886:895	these KLKs	886:895	Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs.					
29975661	4	61	theme	natural	552:558	arg1	proteases					560:568	the natural proteases	548:568	the natural proteases	548:568	In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression.					
29593214	0	0	theme	cell	94:97	arg1	morphology					99:108	bacterial cell morphology	84:108	bacterial cell morphology	84:108	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	3	1	theme	structural	321:330	arg1	material					388:395	a highly ordered, crystalline material	358:395	a highly ordered, crystalline material	358:395	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	3	1	theme	structural	321:330	arg1	model					332:336	The textbook structural model	308:336	The textbook structural model of peptidoglycan	308:353	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	0	2	theme	bacterial	84:92	arg1	morphology					99:108	bacterial cell morphology	84:108	bacterial cell morphology	84:108	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	4	3	from	coli	508:511	arg1	peptidoglycan					477:489	peptidoglycan	477:489	peptidoglycan from Escherichia coli in unprecedented detail	477:535	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	8	4	theme	spheroid	1004:1011	arg1	shape					1013:1017	a spheroid shape	1002:1017	a spheroid shape	1002:1017	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	1	5	from	forces	174:179	arg1	bacteria					202:209	bacteria	202:209	bacteria	202:209	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	1	5	from	forces	174:179	arg1	fungi					192:196	fungi	192:196	fungi	192:196	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	1	5	from	forces	174:179	arg1	plants					184:189	plants	184:189	plants	184:189	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	3	6	theme	ordered	367:373	arg1	material					388:395	a highly ordered, crystalline material	358:395	a highly ordered, crystalline material	358:395	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	3	6	theme	ordered	367:373	arg1	model					332:336	The textbook structural model	308:336	The textbook structural model of peptidoglycan	308:353	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	4	7	theme	atomic	410:415	arg1	AFM					435:437	AFM	435:437	AFM	435:437	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	4	7	theme	atomic	410:415	arg1	microscopy					423:432	atomic force microscopy	410:432	atomic force microscopy (AFM)	410:438	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	4	8	theme	glycan	460:465	arg1	chains					467:472	individual glycan chains	449:472	individual glycan chains	449:472	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	4	9	from	peptidoglycan	477:489	arg1	detail					530:535	unprecedented detail	516:535	unprecedented detail	516:535	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	7	10	from	changes	858:864	arg1	properties					895:904	peptidoglycan biophysical properties	869:904	peptidoglycan biophysical properties	869:904	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
29593214	6	11	dep	chains	769:774	arg1	long					789:792	long	789:792	long	789:792	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	6	12	theme	glycan	762:767	arg1	chains					769:774	glycan chains	762:774	glycan chains up to 200 nm long	762:792	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	7	13	theme	cell	816:819	arg1	shape					821:825	altered cell shape	808:825	altered cell shape	808:825	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
29593214	8	14	from	Glycans	907:913	arg1	shape					946:950	its normal rod shape	931:950	its normal rod shape	931:950	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	0	15	theme	Molecular	0:8	arg1	imaging					10:16	Molecular imaging	0:16	Molecular imaging of glycan chains	0:33	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	5	16	theme	orientational	622:634	arg1	direction					611:619	a similar direction	601:619	a similar direction (orientational order)	601:641	We quantify and map the extent to which chains are oriented in a similar direction (orientational order), showing it is much less ordered than previously depicted.					
29593214	5	16	theme	orientational	622:634	arg1	order					636:640	orientational order	622:640	orientational order	622:640	We quantify and map the extent to which chains are oriented in a similar direction (orientational order), showing it is much less ordered than previously depicted.					
29593214	8	17	theme	rod	942:944	arg1	shape					946:950	its normal rod shape	931:950	its normal rod shape	931:950	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	5	18	theme	similar	603:609	arg1	direction					611:619	a similar direction	601:619	a similar direction (orientational order)	601:641	We quantify and map the extent to which chains are oriented in a similar direction (orientational order), showing it is much less ordered than previously depicted.					
29593214	5	18	theme	similar	603:609	arg1	order					636:640	orientational order	622:640	orientational order	622:640	We quantify and map the extent to which chains are oriented in a similar direction (orientational order), showing it is much less ordered than previously depicted.					
29593214	0	19	theme	glycan	21:26	arg1	chains					28:33	glycan chains	21:33	glycan chains	21:33	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	1	20	theme	Biopolymer	111:120	arg1	walls					137:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	1	21	theme	composite	122:130	arg1	walls					137:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	6	22	dep	long	789:792	arg1	200 nm					782:787	200 nm	782:787	200 nm	782:787	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	2	23	theme	antibiotic	237:246	arg1	target					248:253	a crucial antibiotic target	227:253	a crucial antibiotic target	227:253	Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria.					
29593214	2	23	theme	antibiotic	237:246	arg1	Peptidoglycan					212:224	Peptidoglycan	212:224	Peptidoglycan	212:224	Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria.					
29593214	6	24	dep	200 nm	782:787	arg1	to					779:780	to	779:780	to	779:780	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	0	25	theme	chains	28:33	arg1	imaging					10:16	Molecular imaging	0:16	Molecular imaging of glycan chains	0:33	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	4	26	theme	individual	449:458	arg1	chains					467:472	individual glycan chains	449:472	individual glycan chains	449:472	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	2	27	theme	crucial	229:235	arg1	target					248:253	a crucial antibiotic target	227:253	a crucial antibiotic target	227:253	Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria.					
29593214	2	27	theme	crucial	229:235	arg1	Peptidoglycan					212:224	Peptidoglycan	212:224	Peptidoglycan	212:224	Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria.					
29593214	8	28	from	coli	923:926	arg1	Glycans					907:913	Glycans	907:913	Glycans from E. coli in its normal rod shape	907:950	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	0	29	theme	polysaccharide	53:66	arg1	architecture					68:79	cell-wall polysaccharide architecture	43:79	cell-wall polysaccharide architecture	43:79	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	4	30	theme	unprecedented	516:528	arg1	detail					530:535	unprecedented detail	516:535	unprecedented detail	516:535	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	8	31	dep	induced	1022:1028	arg1	or					1042:1043	or	1042:1043	or	1042:1043	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	1	32	theme	cell	132:135	arg1	walls					137:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls	111:141	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	0	33	theme	cell-wall	43:51	arg1	architecture					68:79	cell-wall polysaccharide architecture	43:79	cell-wall polysaccharide architecture	43:79	Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.					
29593214	3	34	theme	textbook	312:319	arg1	material					388:395	a highly ordered, crystalline material	358:395	a highly ordered, crystalline material	358:395	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	3	34	theme	textbook	312:319	arg1	model					332:336	The textbook structural model	308:336	The textbook structural model of peptidoglycan	308:353	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	6	35	theme	exclusion	726:734	arg1	chromatography					736:749	size exclusion chromatography	721:749	size exclusion chromatography	721:749	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	7	36	theme	biophysical	883:893	arg1	properties					895:904	peptidoglycan biophysical properties	869:904	peptidoglycan biophysical properties	869:904	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
29593214	4	37	theme	force	417:421	arg1	AFM					435:437	AFM	435:437	AFM	435:437	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	4	37	theme	force	417:421	arg1	microscopy					423:432	atomic force microscopy	410:432	atomic force microscopy (AFM)	410:438	Here we use atomic force microscopy (AFM) to image individual glycan chains in peptidoglycan from Escherichia coli in unprecedented detail.					
29593214	6	38	theme	size	721:724	arg1	chromatography					736:749	size exclusion chromatography	721:749	size exclusion chromatography	721:749	Combining AFM with size exclusion chromatography, we reveal glycan chains up to 200 nm long.					
29593214	3	39	dep	ordered	367:373	arg1	crystalline					376:386	crystalline	376:386	crystalline	376:386	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	1	40	theme	cell	152:155	arg1	shape					157:161	cell shape	152:161	cell shape	152:161	Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria.					
29593214	7	41	theme	altered	808:814	arg1	shape					821:825	altered cell shape	808:825	altered cell shape	808:825	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
29593214	7	42	theme	peptidoglycan	869:881	arg1	properties					895:904	peptidoglycan biophysical properties	869:904	peptidoglycan biophysical properties	869:904	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
29593214	3	43	theme	peptidoglycan	341:353	arg1	material					388:395	a highly ordered, crystalline material	358:395	a highly ordered, crystalline material	358:395	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	3	43	theme	peptidoglycan	341:353	arg1	model					332:336	The textbook structural model	308:336	The textbook structural model of peptidoglycan	308:353	The textbook structural model of peptidoglycan is a highly ordered, crystalline material.					
29593214	8	44	theme	normal	935:940	arg1	shape					946:950	its normal rod shape	931:950	its normal rod shape	931:950	Glycans from E. coli in its normal rod shape are long and circumferentially oriented, but when a spheroid shape is induced (chemically or genetically) glycans become short and disordered.					
29593214	7	45	theme	substantial	846:856	arg1	changes					858:864	substantial changes	846:864	substantial changes in peptidoglycan biophysical properties	846:904	We show that altered cell shape is associated with substantial changes in peptidoglycan biophysical properties.					
30397756	0	0	theme	human	81:85	arg1	plasma-correlation					87:104	human plasma-correlation	81:104	human plasma-correlation	81:104	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	6	1	theme	relevant	1011:1018	arg1	disease					1034:1040	relevant chronic liver disease	1011:1040	relevant chronic liver disease	1011:1040	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	5	2	theme	variance	874:881	arg1	analysis					830:837	principal component analysis	810:837	principal component analysis (PCA)	810:843	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	5	2	theme	variance	874:881	arg1	analysis					862:869	multivariate analysis	849:869	multivariate analysis of variance (MANOVA)	849:890	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	5	2	theme	variance	874:881	arg1	PCA					840:842	PCA	840:842	PCA	840:842	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	6	3	theme	chronic	1020:1026	arg1	disease					1034:1040	relevant chronic liver disease	1011:1040	relevant chronic liver disease	1011:1040	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	3	4	theme	intact	474:479	arg1	glycoprotein					523:534	a glycoprotein	521:534	a glycoprotein predominantly produced in liver cells	521:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	4	theme	intact	474:479	arg1	Aα-					481:483	intact Aα-	474:483	intact Aα-	474:483	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	8	5	dep	decreased	1244:1252	arg1	whereas					1271:1277	whereas	1271:1277	whereas	1271:1277	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	3	6	theme	Aα-	481:483	arg1	PTM					466:468	PTM	466:468	PTM	466:468	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	6	theme	Aα-	481:483	arg1	modifications					451:463	the post-translational modifications	428:463	the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells	428:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	10	7	from	disease	1667:1673	arg1	less					1678:1681	less	1678:1681	less	1678:1681	Based on PTM of fibrinogen, starting from plasma we can assign the status of an individual as healthy or as liver disease in less than 3 h.					
30397756	1	8	theme	main	243:246	arg1	factor					253:258	the main risk factor	239:258	the main risk factor being liver cirrhosis (LC)	239:285	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	7	9	theme	robust	1136:1141	arg1	separation					1143:1152	a robust separation	1134:1152	a robust separation between LC and HCC	1134:1171	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
30397756	7	9	theme	robust	1136:1141	arg1	possible					1181:1188	possible	1181:1188	possible	1181:1188	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
30397756	6	10	with	patients	923:930	arg1	disease					943:949	liver disease	937:949	liver disease (HCC and/or LC)	937:965	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	2	11	from	glycoproteins	311:323	arg1	structures					295:304	Glycan structures	288:304	Glycan structures from glycoproteins	288:323	Glycan structures from glycoproteins are usually altered in cancer.					
30397756	3	12	theme	γ-subunits	495:504	arg1	PTM					466:468	PTM	466:468	PTM	466:468	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	12	theme	γ-subunits	495:504	arg1	modifications					451:463	the post-translational modifications	428:463	the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells	428:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	9	13	theme	O-glycans	1392:1400	arg1	amount					1382:1387	the amount	1378:1387	the amount of O-glycans in the Aα-subunit	1378:1418	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	13	theme	O-glycans	1392:1400	arg1	O-glycans					1392:1400	O-glycans	1392:1400	O-glycans	1392:1400	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	1	14	theme	risk	248:251	arg1	factor					253:258	the main risk factor	239:258	the main risk factor being liver cirrhosis (LC)	239:285	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	10	15	theme	fibrinogen	1569:1578	arg1	PTM					1562:1564	PTM	1562:1564	PTM of fibrinogen	1562:1578	Based on PTM of fibrinogen, starting from plasma we can assign the status of an individual as healthy or as liver disease in less than 3 h.					
30397756	5	16	theme	component	820:828	arg1	analysis					830:837	principal component analysis	810:837	principal component analysis (PCA)	810:843	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	5	16	theme	component	820:828	arg1	PCA					840:842	PCA	840:842	PCA	840:842	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	3	17	theme	post-translational	432:449	arg1	PTM					466:468	PTM	466:468	PTM	466:468	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	17	theme	post-translational	432:449	arg1	modifications					451:463	the post-translational modifications	428:463	the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells	428:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	18	theme	Bβ-	486:488	arg1	PTM					466:468	PTM	466:468	PTM	466:468	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	18	theme	Bβ-	486:488	arg1	modifications					451:463	the post-translational modifications	428:463	the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells	428:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	4	19	theme	species	624:630	arg1	phosphorylation					593:607	phosphorylation	593:607	phosphorylation	593:607	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	4	19	theme	species	624:630	arg1	Glycosylation					575:587	Glycosylation	575:587	Glycosylation	575:587	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	0	20	theme	liver	111:115	arg1	cancer					117:122	liver cancer	111:122	liver cancer	111:122	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	9	21	gly	sialylation	1455:1465	arg1	Bβ-					1505:1507	Bβ-	1505:1507	Bβ-	1505:1507	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	21	gly	sialylation	1455:1465	arg1	γ-subunits					1513:1522	γ-subunits	1513:1522	γ-subunits	1513:1522	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	21	gly	sialylation	1455:1465	arg1	HCC					1548:1550	HCC	1548:1550	HCC	1548:1550	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	21	gly	sialylation	1455:1465	arg1	glycans					1494:1500	N-type glycans	1487:1500	N-type glycans	1487:1500	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	21	gly	sialylation	1455:1465	arg1	LC					1541:1542	LC	1541:1542	LC	1541:1542	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	3	22	theme	healthy	378:384	arg1	donors					395:400	111 healthy and sick donors	374:400	111 healthy and sick donors	374:400	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	5	23	theme	statistical	780:790	arg1	classification					792:805	statistical classification	780:805	statistical classification	780:805	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	6	24	theme	%	1049:1049	arg1	sensitivity					1051:1061	91% sensitivity	1047:1061	91% sensitivity	1047:1061	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	4	25	theme	protein	616:622	arg1	species					624:630	the protein species	612:630	the protein species	612:630	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	3	26	theme	Blood	356:360	arg1	plasma					362:367	Blood plasma	356:367	Blood plasma from 111 healthy and sick donors	356:400	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	6	27	theme	91	1047:1048	arg1	%					1049:1049	%	1049:1049	%	1049:1049	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	9	28	gly	fucosylation	1471:1482	arg1	Bβ-					1505:1507	Bβ-	1505:1507	Bβ-	1505:1507	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	28	gly	fucosylation	1471:1482	arg1	γ-subunits					1513:1522	γ-subunits	1513:1522	γ-subunits	1513:1522	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	28	gly	fucosylation	1471:1482	arg1	HCC					1548:1550	HCC	1548:1550	HCC	1548:1550	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	28	gly	fucosylation	1471:1482	arg1	glycans					1494:1500	N-type glycans	1487:1500	N-type glycans	1487:1500	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	28	gly	fucosylation	1471:1482	arg1	LC					1541:1542	LC	1541:1542	LC	1541:1542	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	29	theme	glycans	1494:1500	arg1	fucosylation					1471:1482	fucosylation	1471:1482	fucosylation	1471:1482	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	29	theme	glycans	1494:1500	arg1	sialylation					1455:1465	sialylation	1455:1465	sialylation	1455:1465	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	3	30	theme	sick	390:393	arg1	donors					395:400	111 healthy and sick donors	374:400	111 healthy and sick donors	374:400	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	0	31	theme	Fast	0:3	arg1	analysis					16:23	Fast and facile analysis	0:23	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.	0:143	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	7	32	theme	patients	1105:1112	arg1	patients					1105:1112	patients	1105:1112	patients with liver disease	1105:1131	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
30397756	7	32	theme	patients	1105:1112	arg1	group					1096:1100	the group	1092:1100	the group of patients with liver disease	1092:1131	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
30397756	9	33	theme	Bβ-	1505:1507	arg1	fucosylation					1471:1482	fucosylation	1471:1482	fucosylation	1471:1482	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	33	theme	Bβ-	1505:1507	arg1	sialylation					1455:1465	sialylation	1455:1465	sialylation	1455:1465	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	8	34	theme	more	1194:1197	arg1	detail					1199:1204	more detail	1194:1204	more detail	1194:1204	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	5	35	theme	multivariate	849:860	arg1	analysis					862:869	multivariate analysis	849:869	multivariate analysis of variance (MANOVA)	849:890	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	6	36	theme	liver	937:941	arg1	disease					943:949	liver disease	937:949	liver disease (HCC and/or LC)	937:965	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	0	37	theme	liver	128:132	arg1	cirrhosis					134:142	liver cirrhosis	128:142	liver cirrhosis	128:142	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	4	38	gly	Glycosylation	575:587	arg1	species					624:630	the protein species	612:630	the protein species	612:630	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	4	39	theme	pathological	723:734	arg1	cases					736:740	pathological cases	723:740	pathological cases	723:740	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	9	40	theme	γ-subunits	1513:1522	arg1	fucosylation					1471:1482	fucosylation	1471:1482	fucosylation	1471:1482	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	9	40	theme	γ-subunits	1513:1522	arg1	sialylation					1455:1465	sialylation	1455:1465	sialylation	1455:1465	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	5	41	theme	principal	810:818	arg1	analysis					830:837	principal component analysis	810:837	principal component analysis (PCA)	810:843	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	5	41	theme	principal	810:818	arg1	PCA					840:842	PCA	840:842	PCA	840:842	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	0	42	theme	facile	9:14	arg1	analysis					16:23	Fast and facile analysis	0:23	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.	0:143	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	1	43	theme	liver	266:270	arg1	LC					283:284	LC	283:284	LC	283:284	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	1	43	theme	liver	266:270	arg1	cirrhosis					272:280	liver cirrhosis	266:280	liver cirrhosis (LC)	266:285	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	8	44	theme	monophosphorylated	1283:1300	arg1	state					1302:1306	the monophosphorylated state	1279:1306	the monophosphorylated state	1279:1306	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	3	45	theme	fibrinogen	509:518	arg1	Bβ-					486:488	Bβ-	486:488	Bβ-	486:488	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	45	theme	fibrinogen	509:518	arg1	γ-subunits					495:504	γ-subunits	495:504	γ-subunits	495:504	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	45	theme	fibrinogen	509:518	arg1	Aα-					481:483	intact Aα-	474:483	intact Aα-	474:483	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	45	theme	fibrinogen	509:518	arg1	glycoprotein					523:534	a glycoprotein	521:534	a glycoprotein predominantly produced in liver cells	521:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	9	46	theme	glycosylation	1363:1375	arg1	terms					1354:1358	terms	1354:1358	terms of glycosylation	1354:1375	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	1	47	theme	deadliest	190:198	arg1	cancers					200:206	the deadliest cancers	186:206	the deadliest cancers	186:206	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	0	48	theme	glycosylation	28:40	arg1	analysis					16:23	Fast and facile analysis	0:23	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.	0:143	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	8	49	theme	HCC	1257:1259	arg1	patients					1261:1268	HCC patients	1257:1268	HCC patients	1257:1268	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	6	50	dep	relevant	896:903	arg1	clinical					905:912	clinical	905:912	clinical	905:912	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	8	51	theme	LC	1338:1339	arg1	patients					1341:1348	LC patients	1338:1348	LC patients	1338:1348	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	6	52	theme	liver	1028:1032	arg1	disease					1034:1040	relevant chronic liver disease	1011:1040	relevant chronic liver disease	1011:1040	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	1	53	theme	cancers	200:206	arg1	one					179:181	one	179:181	one	179:181	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	1	53	theme	cancers	200:206	arg1	cancers					200:206	the deadliest cancers	186:206	the deadliest cancers	186:206	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	4	54	theme	liquid	651:656	arg1	chromatography					658:671	liquid chromatography	651:671	liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases	651:740	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	5	55	theme	PTMs	761:764	arg1	Quantities					743:752	Quantities	743:752	Quantities of the PTMs	743:764	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	0	56	with	analysis	16:23	arg1	cirrhosis					134:142	liver cirrhosis	128:142	liver cirrhosis	128:142	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	0	56	with	analysis	16:23	arg1	cancer					117:122	liver cancer	111:122	liver cancer	111:122	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	3	57	gly	glycoprotein	523:534	arg1	glycoprotein					523:534	a glycoprotein	521:534	a glycoprotein predominantly produced in liver cells	521:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	3	57	gly	glycoprotein	523:534	arg1	Aα-					481:483	intact Aα-	474:483	intact Aα-	474:483	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	0	58	theme	phosphorylation	46:60	arg1	analysis					16:23	Fast and facile analysis	0:23	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.	0:143	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	6	59	theme	100	1067:1069	arg1	%					1070:1070	%	1070:1070	%	1070:1070	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	4	60	theme	mass	684:687	arg1	spectrometry					689:700	mass spectrometry	684:700	mass spectrometry to correlate PTMs to pathological cases	684:740	Glycosylation and phosphorylation of the protein species were quantified by liquid chromatography coupled to mass spectrometry to correlate PTMs to pathological cases.					
30397756	9	61	theme	LC	1436:1437	arg1	patients					1439:1446	LC patients	1436:1446	LC patients	1436:1446	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	2	62	gly	glycoproteins	311:323	arg1	glycoproteins					311:323	glycoproteins	311:323	glycoproteins	311:323	Glycan structures from glycoproteins are usually altered in cancer.					
30397756	5	63	used	used	771:774	arg2	Quantities					743:752	Quantities	743:752	Quantities of the PTMs	743:764	Quantities of the PTMs were used for statistical classification by principal component analysis (PCA) and multivariate analysis of variance (MANOVA).					
30397756	6	64	theme	relevant	896:903	arg1	finding					914:920	relevant clinical finding	896:920	relevant clinical finding	896:920	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	6	64	theme	relevant	896:903	arg1	patients					923:930	patients	923:930	patients with liver disease (HCC and/or LC)	923:965	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	9	65	theme	N-type	1487:1492	arg1	glycans					1494:1500	N-type glycans	1487:1500	N-type glycans	1487:1500	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	6	66	with	individuals	991:1001	arg1	specificity					1072:1082	100% specificity	1067:1082	100% specificity	1067:1082	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	6	66	with	individuals	991:1001	arg1	sensitivity					1051:1061	91% sensitivity	1047:1061	91% sensitivity	1047:1061	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	3	67	from	donors	395:400	arg1	plasma					362:367	Blood plasma	356:367	Blood plasma from 111 healthy and sick donors	356:400	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	6	68	theme	%	1070:1070	arg1	specificity					1072:1082	100% specificity	1067:1082	100% specificity	1067:1082	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	7	69	with	patients	1105:1112	arg1	disease					1125:1131	liver disease	1119:1131	liver disease	1119:1131	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
30397756	6	70	dep	disease	943:949	arg1	LC					963:964	LC	963:964	LC	963:964	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	6	70	dep	disease	943:949	arg1	HCC					952:954	HCC	952:954	HCC	952:954	As relevant clinical finding, patients with liver disease (HCC and/or LC) were distinguished from individuals without relevant chronic liver disease with 91% sensitivity and 100% specificity.					
30397756	0	71	gly	glycosylation	28:40	arg1	fibrinogen					65:74	fibrinogen	65:74	fibrinogen	65:74	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	9	72	from	amount	1382:1387	arg1	Aα-subunit					1409:1418	the Aα-subunit	1405:1418	the Aα-subunit	1405:1418	In terms of glycosylation, the amount of O-glycans in the Aα-subunit is decreased in LC patients, while sialylation and fucosylation of N-type glycans of Bβ- and γ-subunits are increased in LC and HCC.					
30397756	2	73	theme	Glycan	288:293	arg1	structures					295:304	Glycan structures	288:304	Glycan structures from glycoproteins	288:323	Glycan structures from glycoproteins are usually altered in cancer.					
30397756	10	74	theme	liver	1661:1665	arg1	disease					1667:1673	liver disease	1661:1673	liver disease in less than 3 h	1661:1690	Based on PTM of fibrinogen, starting from plasma we can assign the status of an individual as healthy or as liver disease in less than 3 h.					
30397756	0	75	theme	fibrinogen	65:74	arg1	glycosylation					28:40	glycosylation	28:40	glycosylation	28:40	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	0	75	theme	fibrinogen	65:74	arg1	phosphorylation					46:60	phosphorylation	46:60	phosphorylation	46:60	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	0	76	from	plasma-correlation	87:104	arg1	glycosylation					28:40	glycosylation	28:40	glycosylation	28:40	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	0	76	from	plasma-correlation	87:104	arg1	analysis					16:23	Fast and facile analysis	0:23	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.	0:143	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	0	76	from	plasma-correlation	87:104	arg1	phosphorylation					46:60	phosphorylation	46:60	phosphorylation	46:60	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	3	77	theme	liver	562:566	arg1	cells					568:572	liver cells	562:572	liver cells	562:572	Blood plasma from 111 healthy and sick donors was analyzed to determine the post-translational modifications (PTM) of intact Aα-, Bβ-, and γ-subunits of fibrinogen, a glycoprotein predominantly produced in liver cells.					
30397756	1	78	with	diagnosis	224:232	arg1	factor					253:258	the main risk factor	239:258	the main risk factor being liver cirrhosis (LC)	239:285	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	1	79	theme	Hepatocellular	145:158	arg1	carcinoma					160:168	Hepatocellular carcinoma	145:168	Hepatocellular carcinoma (HCC)	145:174	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	1	79	theme	Hepatocellular	145:158	arg1	HCC					171:173	HCC	171:173	HCC	171:173	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	8	80	theme	Aα-subunit	1230:1239	arg1	phosphorylation					1211:1225	the phosphorylation	1207:1225	the phosphorylation of Aα-subunit	1207:1239	In more detail, the phosphorylation of Aα-subunit is decreased in HCC patients, whereas the monophosphorylated state is significantly increased in LC patients.					
30397756	1	81	theme	late	219:222	arg1	diagnosis					224:232	its late diagnosis	215:232	its late diagnosis with the main risk factor being liver cirrhosis (LC)	215:285	Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its late diagnosis with the main risk factor being liver cirrhosis (LC).					
30397756	10	82	theme	individual	1633:1642	arg1	status					1620:1625	the status	1616:1625	the status of an individual as healthy or as liver disease in less than 3 h	1616:1690	Based on PTM of fibrinogen, starting from plasma we can assign the status of an individual as healthy or as liver disease in less than 3 h.					
30397756	0	83	from	analysis	16:23	arg1	plasma-correlation					87:104	human plasma-correlation	81:104	human plasma-correlation	81:104	Fast and facile analysis of glycosylation and phosphorylation of fibrinogen from human plasma-correlation with liver cancer and liver cirrhosis.					
30397756	7	84	theme	liver	1119:1123	arg1	disease					1125:1131	liver disease	1119:1131	liver disease	1119:1131	Within the group of patients with liver disease, a robust separation between LC and HCC was not possible.					
31057084	8	0	theme	turns.Communicated	1084:1101	arg1	epitope					1064:1070	the antibody interacting epitope	1039:1070	the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma	1039:1123	Glycan changes the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma.					
31057084	7	1	theme	water	883:887	arg1	molecules					889:897	the water molecules	879:897	the water molecules	879:897	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	0	2	theme	glycoprotein	69:80	arg1	changes					35:41	the structural changes	20:41	the structural changes of myelin oligodendrocyte glycoprotein	20:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	4	3	theme	dynamics	470:477	arg1	simulation					479:488	the molecular dynamics simulation studies	456:496	the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody	456:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	7	4	theme	free	907:910	arg1	interaction					924:934	free protein MOG interaction	907:934	free protein MOG interaction with water molecules	907:955	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	1	5	theme	important	225:233	arg1	role					235:238	an important role	222:238	an important role	222:238	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	6	from	Role	0:3	arg1	changes					35:41	the structural changes	20:41	the structural changes of myelin oligodendrocyte glycoprotein	20:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	7	7	theme	protein	912:918	arg1	interaction					924:934	free protein MOG interaction	907:934	free protein MOG interaction with water molecules	907:955	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	5	8	theme	glycosylation	665:677	arg1	effect					655:660	the effect	651:660	the effect of glycosylation in the MOG conformational changes	651:711	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	4	9	dep	simulation	479:488	arg1	studies					490:496	studies	490:496	the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody	456:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	1	10	theme	Oligodendrocyte	123:137	arg1	MOG					153:155	MOG	153:155	MOG	153:155	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	1	10	theme	Oligodendrocyte	123:137	arg1	Glycoprotein					139:150	Myelin Oligodendrocyte Glycoprotein	116:150	Myelin Oligodendrocyte Glycoprotein (MOG)	116:156	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	7	11	with	interaction	847:857	arg1	molecules					889:897	the water molecules	879:897	the water molecules	879:897	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	6	12	from	flexibility	772:782	arg1	states					818:823	both free and bound states	798:823	both free and bound states	798:823	The results indicate that the glycosylation decreases the flexibility of protein in both free and bound states.					
31057084	0	13	gly	glycoprotein	69:80	arg1	glycoprotein					69:80	myelin oligodendrocyte glycoprotein	46:80	myelin oligodendrocyte glycoprotein	46:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	3	14	theme	protein	396:402	arg1	change					386:391	the sudden change	375:391	the sudden change of protein	375:402	The cause for the sudden change of protein to be autoantigen is still unclear.					
31057084	4	15	from	simulation	479:488	arg1	states					531:536	both unbound and bound states	508:536	both unbound and bound states with an antibody	508:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	7	16	with	interaction	924:934	arg1	molecules					947:955	water molecules	941:955	water molecules	941:955	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	8	17	dep	turns.Communicated	1084:1101	arg1	to					1081:1082	to	1081:1082	to	1081:1082	Glycan changes the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma.					
31057084	5	18	theme	MOG	686:688	arg1	changes					705:711	the MOG conformational changes	682:711	the MOG conformational changes	682:711	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	8	19	theme	antibody	1043:1050	arg1	epitope					1064:1070	the antibody interacting epitope	1039:1070	the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma	1039:1123	Glycan changes the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma.					
31057084	1	20	theme	neurodegenerative	243:259	arg1	diseases					261:268	neurodegenerative diseases	243:268	neurodegenerative diseases	243:268	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	21	with	complex	90:96	arg1	antibody					106:113	an antibody	103:113	an antibody	103:113	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	7	22	theme	complex	866:872	arg1	interaction					847:857	the interaction	843:857	the interaction of the complex with the water molecules	843:897	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	8	23	theme	adjacent	1027:1034	arg1	helices					1019:1025	the 310 helices	1011:1025	the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma	1011:1123	Glycan changes the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma.					
31057084	5	24	from	effect	655:660	arg1	changes					705:711	the MOG conformational changes	682:711	the MOG conformational changes	682:711	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	1	25	theme	external	174:181	arg1	surface					183:189	the external surface	170:189	the external surface of the myelin sheath	170:210	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	26	theme	N-glycan	8:15	arg1	complex					90:96	its complex	86:96	its complex with an antibody	86:113	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	0	26	theme	N-glycan	8:15	arg1	Role					0:3	Role	0:3	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein	0:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	1	27	located	found	161:165	arg1	surface					183:189	the external surface	170:189	the external surface of the myelin sheath	170:210	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	1	27	located	found	161:165	arg2	MOG					153:155	MOG	153:155	MOG	153:155	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	1	27	located	found	161:165	arg2	Glycoprotein					139:150	Myelin Oligodendrocyte Glycoprotein	116:150	Myelin Oligodendrocyte Glycoprotein (MOG)	116:156	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	28	theme	structural	24:33	arg1	changes					35:41	the structural changes	20:41	the structural changes of myelin oligodendrocyte glycoprotein	20:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	4	29	theme	unbound	513:519	arg1	states					531:536	both unbound and bound states	508:536	both unbound and bound states with an antibody	508:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	3	30	theme	sudden	379:384	arg1	change					386:391	the sudden change	375:391	the sudden change of protein	375:402	The cause for the sudden change of protein to be autoantigen is still unclear.					
31057084	4	31	theme	bound	525:529	arg1	states					531:536	both unbound and bound states	508:536	both unbound and bound states with an antibody	508:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	6	32	theme	free	803:806	arg1	states					818:823	both free and bound states	798:823	both free and bound states	798:823	The results indicate that the glycosylation decreases the flexibility of protein in both free and bound states.					
31057084	8	33	theme	interacting	1052:1062	arg1	epitope					1064:1070	the antibody interacting epitope	1039:1070	the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma	1039:1123	Glycan changes the 310 helices adjacent to the antibody interacting epitope MOG35-55 to turns.Communicated by Ramaswamy H. Sarma.					
31057084	5	34	theme	conformational	690:703	arg1	changes					705:711	the MOG conformational changes	682:711	the MOG conformational changes	682:711	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	6	35	theme	bound	812:816	arg1	states					818:823	both free and bound states	798:823	both free and bound states	798:823	The results indicate that the glycosylation decreases the flexibility of protein in both free and bound states.					
31057084	4	36	theme	molecular	460:468	arg1	simulation					479:488	the molecular dynamics simulation studies	456:496	the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody	456:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	4	37	theme	MOG	501:503	arg1	simulation					479:488	the molecular dynamics simulation studies	456:496	the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody	456:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	5	38	theme	N-glycan	619:626	arg1	absence					595:601	absence	595:601	absence	595:601	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	5	38	theme	N-glycan	619:626	arg1	presence					607:614	presence	607:614	presence	607:614	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	6	39	theme	protein	787:793	arg1	flexibility					772:782	the flexibility	768:782	the flexibility of protein in both free and bound states	768:823	The results indicate that the glycosylation decreases the flexibility of protein in both free and bound states.					
31057084	0	40	with	Role	0:3	arg1	antibody					106:113	an antibody	103:113	an antibody	103:113	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	1	41	theme	Myelin	116:121	arg1	MOG					153:155	MOG	153:155	MOG	153:155	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	1	41	theme	Myelin	116:121	arg1	Glycoprotein					139:150	Myelin Oligodendrocyte Glycoprotein	116:150	Myelin Oligodendrocyte Glycoprotein (MOG)	116:156	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	1	42	theme	myelin	198:203	arg1	sheath					205:210	the myelin sheath	194:210	the myelin sheath	194:210	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	43	from	complex	90:96	arg1	changes					35:41	the structural changes	20:41	the structural changes of myelin oligodendrocyte glycoprotein	20:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	2	44	theme	protein	296:302	arg1	autoantigen					319:329	an autoantigen	316:329	an autoantigen	316:329	It was observed that the protein MOG acts as an autoantigen and results in demyelination.					
31057084	2	44	theme	protein	296:302	arg1	MOG					304:306	the protein MOG	292:306	the protein MOG	292:306	It was observed that the protein MOG acts as an autoantigen and results in demyelination.					
31057084	1	45	theme	sheath	205:210	arg1	surface					183:189	the external surface	170:189	the external surface of the myelin sheath	170:210	Myelin Oligodendrocyte Glycoprotein (MOG) is found on the external surface of the myelin sheath and plays an important role in neurodegenerative diseases.					
31057084	0	46	theme	oligodendrocyte	53:67	arg1	glycoprotein					69:80	myelin oligodendrocyte glycoprotein	46:80	myelin oligodendrocyte glycoprotein	46:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	4	47	with	states	531:536	arg1	antibody					546:553	an antibody	543:553	an antibody	543:553	Here we present the molecular dynamics simulation studies of MOG in both unbound and bound states with an antibody.					
31057084	7	48	theme	water	941:945	arg1	molecules					947:955	water molecules	941:955	water molecules	941:955	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31057084	0	49	theme	myelin	46:51	arg1	glycoprotein					69:80	myelin oligodendrocyte glycoprotein	46:80	myelin oligodendrocyte glycoprotein	46:80	Role of N-glycan in the structural changes of myelin oligodendrocyte glycoprotein and its complex with an antibody.					
31057084	5	50	dep	absence	595:601	arg1	the					591:593	the	591:593	the	591:593	Both these systems were studied in the absence and presence of N-glycan in order to understand the effect of glycosylation in the MOG conformational changes.					
31057084	7	51	theme	MOG	920:922	arg1	interaction					924:934	free protein MOG interaction	907:934	free protein MOG interaction with water molecules	907:955	Glycan influence the interaction of the complex with the water molecules whereas free protein MOG interaction with water molecules was not affected by the glycosylation.					
31593866	1	0	theme	glycoproteins	370:382	arg1	N-glycosylation					339:353	the N-glycosylation	335:353	the N-glycosylation of therapeutic glycoproteins	335:382	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	1	1	gly	N-glycosylation	339:353	arg1	glycoproteins					370:382	therapeutic glycoproteins	358:382	therapeutic glycoproteins	358:382	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	2	2	theme	glycosylation	395:407	arg1	pattern					409:415	the glycosylation pattern	391:415	the glycosylation pattern of glycobiotherapeutics	391:439	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	1	3	theme	reproducible	254:265	arg1	methods					299:305	reproducible high throughput glycoanalytical methods	254:305	reproducible high throughput glycoanalytical methods	254:305	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	2	4	theme	high	558:561	arg1	importance					563:572	high importance	558:572	high importance	558:572	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	1	5	theme	high	267:270	arg1	methods					299:305	reproducible high throughput glycoanalytical methods	254:305	reproducible high throughput glycoanalytical methods	254:305	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	3	6	theme	multicapillary	608:621	arg1	electrophoresis					627:641	multicapillary gel electrophoresis	608:641	multicapillary gel electrophoresis	608:641	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	1	7	theme	throughput	272:281	arg1	methods					299:305	reproducible high throughput glycoanalytical methods	254:305	reproducible high throughput glycoanalytical methods	254:305	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	3	8	theme	gel	623:625	arg1	electrophoresis					627:641	multicapillary gel electrophoresis	608:641	multicapillary gel electrophoresis	608:641	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	1	9	gly	glycoproteins	370:382	arg1	glycoproteins					370:382	therapeutic glycoproteins	358:382	therapeutic glycoproteins	358:382	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	1	10	theme	glycoanalytical	283:297	arg1	methods					299:305	reproducible high throughput glycoanalytical methods	254:305	reproducible high throughput glycoanalytical methods	254:305	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	0	11	theme	glucose	122:128	arg1	database					135:142	a new glucose unit database	116:142	a new glucose unit database	116:142	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	2	12	theme	important	458:466	arg1	functions					479:487	their important biological functions	452:487	their important biological functions	452:487	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	0	13	theme	new	118:120	arg1	database					135:142	a new glucose unit database	116:142	a new glucose unit database	116:142	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	14	theme	carbohydrate	811:822	arg1	structures					824:833	the carbohydrate structures	807:833	the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	807:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	15	theme	high	646:649	arg1	analysis					662:669	high throughput analysis	646:669	high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	646:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	0	16	theme	N-glycosylation	0:14	arg1	analysis					16:23	N-glycosylation analysis	0:23	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.	0:143	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	17	theme	profile	847:853	arg1	adalimumab					875:884	adalimumab	875:884	adalimumab	875:884	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	17	theme	profile	847:853	arg1	biopharmaceuticals					855:872	two high profile biopharmaceuticals	838:872	two high profile biopharmaceuticals	838:872	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	17	theme	profile	847:853	arg1	etanercept					890:899	etanercept	890:899	etanercept	890:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	18	theme	throughput	651:660	arg1	analysis					662:669	high throughput analysis	646:669	high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	646:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	1	19	theme	biopharmaceutical	194:210	arg1	industry					212:219	the biopharmaceutical industry	190:219	the biopharmaceutical industry	190:219	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	0	20	theme	biopharmaceuticals	28:45	arg1	analysis					16:23	N-glycosylation analysis	0:23	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.	0:143	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	21	theme	biopharmaceuticals	855:872	arg1	structures					824:833	the carbohydrate structures	807:833	the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	807:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	0	22	theme	database	135:142	arg1	application					101:111	application	101:111	application	101:111	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	0	22	theme	database	135:142	arg1	Generation					86:95	Generation	86:95	Generation	86:95	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	23	theme	electrophoresis	627:641	arg1	use					601:603	the use	597:603	the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	597:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	24	theme	fluorophore	674:684	arg1	analysis					662:669	high throughput analysis	646:669	high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	646:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	0	25	theme	unit	130:133	arg1	database					135:142	a new glucose unit database	116:142	a new glucose unit database	116:142	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	26	theme	new	739:741	arg1	database					756:763	a new glucose unit database	737:763	a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	737:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	0	27	theme	multicapillary	50:63	arg1	electrophoresis					69:83	multicapillary gel electrophoresis	50:83	multicapillary gel electrophoresis	50:83	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	3	28	theme	high	842:845	arg1	adalimumab					875:884	adalimumab	875:884	adalimumab	875:884	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	28	theme	high	842:845	arg1	biopharmaceuticals					855:872	two high profile biopharmaceuticals	838:872	two high profile biopharmaceuticals	838:872	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	28	theme	high	842:845	arg1	etanercept					890:899	etanercept	890:899	etanercept	890:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	29	theme	glucose	743:749	arg1	database					756:763	a new glucose unit database	737:763	a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	737:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	0	30	gly	N-glycosylation	0:14	arg1	biopharmaceuticals					28:45	biopharmaceuticals	28:45	biopharmaceuticals	28:45	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	2	31	theme	glycobiotherapeutics	420:439	arg1	pattern					409:415	the glycosylation pattern	391:415	the glycosylation pattern of glycobiotherapeutics	391:439	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	2	32	gly	glycosylation	395:407	arg1	glycobiotherapeutics					420:439	glycobiotherapeutics	420:439	glycobiotherapeutics	420:439	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	2	33	theme	biological	468:477	arg1	functions					479:487	their important biological functions	452:487	their important biological functions	452:487	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	0	34	theme	gel	65:67	arg1	electrophoresis					69:83	multicapillary gel electrophoresis	50:83	multicapillary gel electrophoresis	50:83	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	2	35	theme	complex	534:540	arg1	molecules					542:550	these complex molecules	528:550	these complex molecules	528:550	Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance.					
31593866	3	36	theme	partitioned	694:704	arg1	libraries					715:723	partitioned N-glycan libraries	694:723	partitioned N-glycan libraries	694:723	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	37	theme	unit	751:754	arg1	database					756:763	a new glucose unit database	737:763	a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	737:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	1	38	theme	increasing	234:243	arg1	need					245:248	an increasing need	231:248	an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins	231:382	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	0	39	dep	analysis	16:23	arg1	application					101:111	application	101:111	application	101:111	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	0	39	dep	analysis	16:23	arg1	Generation					86:95	Generation	86:95	Generation	86:95	N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database.					
31593866	1	40	theme	glycomics	148:156	arg1	research					158:165	glycomics research	148:165	glycomics research	148:165	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	1	41	theme	therapeutic	358:368	arg1	glycoproteins					370:382	therapeutic glycoproteins	358:382	therapeutic glycoproteins	358:382	As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins.					
31593866	3	42	theme	N-glycan	706:713	arg1	libraries					715:723	partitioned N-glycan libraries	694:723	partitioned N-glycan libraries	694:723	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
31593866	3	43	from	use	601:603	arg1	analysis					662:669	high throughput analysis	646:669	high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept	646:899	This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.					
30640124	6	0	with	hospitals	1132:1140	arg1	potential					1159:1167	higher potential	1152:1167	higher potential	1152:1167	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	6	1	from	levels	1054:1059	arg1	serum					1071:1075	serum	1071:1075	serum	1071:1075	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	1	2	theme	chemical	151:158	arg1	sensors					160:166	chemical sensors	151:166	chemical sensors capable of detecting the specific glycosylation patterns of proteins	151:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	5	3	with	sensor	815:820	arg1	format					838:843	a sandwich format	827:843	a sandwich format employing the identified aptamer as a signaling receptor	827:900	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	4	theme	identified	859:868	arg1	aptamer					870:876	the identified aptamer	855:876	the identified aptamer	855:876	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	4	theme	identified	859:868	arg1	receptor					893:900	a signaling receptor	881:900	a signaling receptor	881:900	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	1	5	theme	sensors	160:166	arg1	development					136:146	The development	132:146	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins	132:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	0	6	theme	specific	93:100	arg1	detection					102:110	more specific detection	88:110	more specific detection of prostate cancer	88:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	6	7	theme	higher	1152:1157	arg1	potential					1159:1167	higher potential	1152:1167	higher potential	1152:1167	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	7	8	theme	sensor	1326:1331	arg1	demonstration					1292:1304	the first demonstration	1282:1304	the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety	1282:1378	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	7	8	theme	sensor	1326:1331	arg1	this					1274:1277	this	1274:1277	this	1274:1277	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	4	9	theme	reasonable	719:728	arg1	affinity					730:737	reasonable affinity	719:737	reasonable affinity (a dissociation constant of 177 ± 65 nM)	719:778	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	6	10	theme	different	1044:1052	arg1	levels					1054:1059	different levels	1044:1059	different levels of PSA in serum	1044:1075	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	5	11	theme	discriminating	922:935	arg1	PSA					943:945	discriminating human PSA	922:945	discriminating human PSA from the unglycosylated protein	922:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	1	12	theme	early	268:272	arg1	detection					274:282	the early detection	264:282	the early detection of cancer	264:292	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	1	13	gly	glycosylation	202:214	arg1	proteins					228:235	proteins	228:235	proteins	228:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	3	14	theme	simple	463:468	arg1	method					470:475	a simple method	461:475	a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins	461:564	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	3	14	theme	simple	463:468	arg1	target					615:620	a model target	607:620	a model target	607:620	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	5	15	theme	human	937:941	arg1	PSA					943:945	discriminating human PSA	922:945	discriminating human PSA from the unglycosylated protein	922:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	16	from	protein	971:977	arg1	tool					914:917	a tool	912:917	a tool of discriminating human PSA from the unglycosylated protein	912:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	16	from	protein	971:977	arg1	PSA					943:945	discriminating human PSA	922:945	discriminating human PSA from the unglycosylated protein	922:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	1	17	theme	capable	168:174	arg1	sensors					160:166	chemical sensors	151:166	chemical sensors capable of detecting the specific glycosylation patterns of proteins	151:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	7	18	from	validation	1233:1242	arg1	cohort					1256:1261	a larger cohort	1247:1261	a larger cohort	1247:1261	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	3	19	theme	glycan	528:533	arg1	structure					535:543	the glycan structure	524:543	the glycan structure of the glycoproteins	524:564	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	7	20	theme	first	1286:1290	arg1	demonstration					1292:1304	the first demonstration	1282:1304	the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety	1282:1378	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	7	20	theme	first	1286:1290	arg1	this					1274:1277	this	1274:1277	this	1274:1277	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	0	21	theme	aptamer	9:15	arg1	selection					17:25	aptamer selection	9:25	aptamer selection	9:25	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	6	22	theme	chemiluminescence	1100:1116	arg1	ELISA					1118:1122	chemiluminescence ELISA	1100:1122	chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer	1100:1221	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	5	23	theme	sandwich	829:836	arg1	format					838:843	a sandwich format	827:843	a sandwich format employing the identified aptamer as a signaling receptor	827:900	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	6	24	theme	prostate	1207:1214	arg1	cancer					1216:1221	clinically meaningful prostate cancer	1185:1221	clinically meaningful prostate cancer	1185:1221	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	4	25	theme	glycan	693:698	arg1	moiety					700:705	the glycan moiety	689:705	the glycan moiety of PSA	689:712	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	7	26	theme	aptamer-based	1312:1324	arg1	sensor					1326:1331	an aptamer-based sensor	1309:1331	an aptamer-based sensor	1309:1331	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	0	27	theme	cancer	124:129	arg1	detection					102:110	more specific detection	88:110	more specific detection of prostate cancer	88:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	4	28	theme	PSA	710:712	arg1	moiety					700:705	the glycan moiety	689:705	the glycan moiety of PSA	689:712	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	1	29	theme	specific	193:200	arg1	patterns					216:223	the specific glycosylation patterns	189:223	the specific glycosylation patterns of proteins	189:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	1	30	theme	cancer	287:292	arg1	detection					274:282	the early detection	264:282	the early detection of cancer	264:292	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	0	31	theme	prostate	115:122	arg1	cancer					124:129	prostate cancer	115:129	prostate cancer	115:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	3	32	theme	glycoproteins	552:564	arg1	structure					535:543	the glycan structure	524:543	the glycan structure of the glycoproteins	524:564	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	1	33	theme	glycosylation	202:214	arg1	patterns					216:223	the specific glycosylation patterns	189:223	the specific glycosylation patterns of proteins	189:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	0	34	theme	glycan	34:39	arg1	structure					41:49	the glycan structure	30:49	the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer	30:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	6	35	theme	meaningful	1196:1205	arg1	cancer					1216:1221	clinically meaningful prostate cancer	1185:1221	clinically meaningful prostate cancer	1185:1221	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	5	36	theme	electrochemical	799:813	arg1	sensor					815:820	an electrochemical sensor	796:820	an electrochemical sensor	796:820	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	4	37	theme	177 ± 65 nM	767:777	arg1	constant					755:762	a dissociation constant	740:762	a dissociation constant of 177 ± 65 nM	740:777	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	3	38	theme	prostate-specific	572:588	arg1	PSA					599:601	PSA	599:601	PSA	599:601	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	3	38	theme	prostate-specific	572:588	arg1	antigen					590:596	prostate-specific antigen	572:596	prostate-specific antigen (PSA)	572:602	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	5	39	gly	unglycosylated	956:969	arg1	protein					971:977	the unglycosylated protein	952:977	the unglycosylated protein	952:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	7	40	theme	larger	1249:1254	arg1	cohort					1256:1261	a larger cohort	1247:1261	a larger cohort	1247:1261	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	5	41	theme	detection	996:1004	arg1	limit					987:991	a limit	985:991	a limit of detection of 0.66 ng/mL	985:1018	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	4	42	theme	dissociation	742:753	arg1	constant					755:762	a dissociation constant	740:762	a dissociation constant of 177 ± 65 nM	740:777	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	7	43	theme	glycan	1366:1371	arg1	moiety					1373:1378	its glycan moiety	1362:1378	its glycan moiety	1362:1378	Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.					
30640124	3	44	gly	glycoproteins	552:564	arg1	glycoproteins					552:564	the glycoproteins	548:564	the glycoproteins	548:564	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	5	45	from	tool	914:917	arg1	protein					971:977	the unglycosylated protein	952:977	the unglycosylated protein	952:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	1	46	theme	proteins	228:235	arg1	patterns					216:223	the specific glycosylation patterns	189:223	the specific glycosylation patterns of proteins	189:235	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
30640124	5	47	theme	PSA	943:945	arg1	tool					914:917	a tool	912:917	a tool of discriminating human PSA from the unglycosylated protein	912:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	2	48	attach	linked	387:392	arg2	glycans					379:385	the glycans	375:385	the glycans linked to proteins	375:404	Unfortunately, this strategy is scarcely explored because receptors recognizing the glycans linked to proteins are challenging to discover.					
30640124	2	48	attach	linked	387:392	arg1	proteins					397:404	proteins	397:404	proteins	397:404	Unfortunately, this strategy is scarcely explored because receptors recognizing the glycans linked to proteins are challenging to discover.					
30640124	3	49	theme	model	609:613	arg1	target					615:620	a model target	607:620	a model target	607:620	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	3	49	theme	model	609:613	arg1	method					470:475	a simple method	461:475	a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins	461:564	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	6	50	theme	PSA	1064:1066	arg1	levels					1054:1059	different levels	1044:1059	different levels of PSA in serum	1044:1075	The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer.					
30640124	5	51	theme	signaling	883:891	arg1	aptamer					870:876	the identified aptamer	855:876	the identified aptamer	855:876	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	51	theme	signaling	883:891	arg1	receptor					893:900	a signaling receptor	881:900	a signaling receptor	881:900	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	0	52	theme	antigen	72:78	arg1	structure					41:49	the glycan structure	30:49	the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer	30:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	5	53	theme	0.66 ng/mL	1009:1018	arg1	detection					996:1004	detection	996:1004	detection of 0.66 ng/mL	996:1018	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	5	54	theme	unglycosylated	956:969	arg1	protein					971:977	the unglycosylated protein	952:977	the unglycosylated protein	952:977	Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL.					
30640124	3	55	theme	aptamers	508:515	arg1	selection					495:503	the selection	491:503	the selection of aptamers	491:515	In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target.					
30640124	0	56	theme	prostate-specific	54:70	arg1	antigen					72:78	prostate-specific antigen:	54:79	prostate-specific antigen: Toward more specific detection of prostate cancer	54:129	Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.					
30640124	4	57	dep	affinity	730:737	arg1	constant					755:762	a dissociation constant	740:762	a dissociation constant of 177 ± 65 nM	740:777	Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM).					
30640124	1	58	theme	powerful	246:253	arg1	mean					255:258	a powerful mean	244:258	a powerful mean for the early detection of cancer	244:292	The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer.					
29915149	7	0	theme	substantia	1442:1451	arg1	nigra					1453:1457	substantia nigra	1442:1457	substantia nigra	1442:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	10	1	theme	Disease	1890:1896	arg1	therapy					1908:1914	Parkinson Disease (PD) gene therapy	1880:1914	Parkinson Disease (PD) gene therapy	1880:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	6	2	from	HS	1179:1180	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	2	from	HS	1179:1180	arg1	nigra					1273:1277	young versus aged rat brain striatum and substantia nigra	1221:1277	nigra	1273:1277	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	7	3	theme	adult	1377:1381	arg1	rats					1383:1386	young adult rats	1371:1386	young adult rats	1371:1386	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	4	4	theme	primary	718:724	arg1	glycans					757:763	glycans	757:763	glycans	757:763	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	4	4	theme	primary	718:724	arg1	receptors					730:738	all primary AAV receptors	714:738	all primary AAV receptors of the virus	714:751	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	6	5	theme	proteomic	1197:1205	arg1	signatures					1207:1216	proteomic signatures	1197:1216	proteomic signatures	1197:1216	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	2	6	theme	symptomatic	278:288	arg1	relief					290:295	only symptomatic relief	273:295	only symptomatic relief	273:295	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	6	7	from	N-glycans	1183:1191	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	7	from	N-glycans	1183:1191	arg1	nigra					1273:1277	young versus aged rat brain striatum and substantia nigra	1221:1277	nigra	1273:1277	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	9	8	theme	brain	1631:1635	arg1	changes					1653:1659	brain region-specific changes	1631:1659	brain region-specific changes	1631:1659	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	2	9	theme	nigrostriatal	365:377	arg1	neurons					388:394	nigrostriatal dopamine neurons	365:394	nigrostriatal dopamine neurons	365:394	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	10	10	theme	gene	1903:1906	arg1	therapy					1908:1914	Parkinson Disease (PD) gene therapy	1880:1914	Parkinson Disease (PD) gene therapy	1880:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	5	11	theme	heparan	834:840	arg1	HS					851:852	HS	851:852	HS	851:852	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	5	11	theme	heparan	834:840	arg1	sulfate					842:848	heparan sulfate	834:848	heparan sulfate (HS) proteoglycans (receptor for rAAV2)	834:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	7	12	theme	brain	1392:1396	arg1	profiles					1414:1421	brain region-specific profiles	1392:1421	brain region-specific profiles in striatum versus substantia nigra	1392:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	1	13	theme	dopaminergic	173:184	arg1	neurons					186:192	functional dopaminergic neurons	162:192	functional dopaminergic neurons	162:192	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	9	14	theme	protein	1664:1670	arg1	expression					1672:1681	protein expression	1664:1681	protein expression in the aging nigrostriatal pathway	1664:1716	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	6	15	theme	substantia	1262:1271	arg1	nigra					1273:1277	young versus aged rat brain striatum and substantia nigra	1221:1277	nigra	1273:1277	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	7	16	theme	HS	1324:1325	arg1	profiles					1340:1347	different brain region-specific HS disaccharide profiles	1292:1347	different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra	1292:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	6	17	dep	analyzed	1121:1128	arg1	test					1098:1101	test	1098:1101	To test our hypothesis	1095:1116	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	5	18	theme	substantia	1026:1035	arg1	nigra					1037:1041	substantia nigra	1026:1041	substantia nigra	1026:1041	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	1	19	theme	nigrostriatal	201:213	arg1	pathway					215:221	the nigrostriatal pathway	197:221	the nigrostriatal pathway in the brain	197:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	9	20	theme	aging	1690:1694	arg1	pathway					1710:1716	the aging nigrostriatal pathway	1686:1716	the aging nigrostriatal pathway	1686:1716	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	7	21	theme	brain	1302:1306	arg1	profiles					1340:1347	different brain region-specific HS disaccharide profiles	1292:1347	different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra	1292:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	1	22	from	pathway	215:221	arg1	brain					230:234	the brain	226:234	the brain	226:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	6	23	dep	striatum	1134:1141	arg1	the					1130:1132	the	1130:1132	the	1130:1132	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	24	theme	rat	1239:1241	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	10	25	theme	region-specific	1769:1783	arg1	changes					1785:1791	brain region-specific changes	1763:1791	brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy	1763:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	5	26	from	changes	803:809	arg1	receptors					821:829	glycan receptors	814:829	glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2)	814:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	7	27	from	profiles	1340:1347	arg1	aged					1352:1355	aged	1352:1355	aged	1352:1355	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	6	28	theme	substantia	1147:1156	arg1	nigra					1158:1162	substantia nigra	1147:1162	substantia nigra	1147:1162	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	2	29	theme	current	246:252	arg1	treatments					254:263	current treatments	246:263	current treatments	246:263	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	6	30	from	changes	1168:1174	arg1	N-glycans					1183:1191	N-glycans	1183:1191	N-glycans	1183:1191	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	30	from	changes	1168:1174	arg1	signatures					1207:1216	proteomic signatures	1197:1216	proteomic signatures	1197:1216	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	30	from	changes	1168:1174	arg1	HS					1179:1180	HS	1179:1180	HS	1179:1180	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	5	31	theme	related	795:801	arg1	changes					803:809	age related changes	791:809	age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2)	791:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	1	32	from	loss	154:157	arg1	pathway					215:221	the nigrostriatal pathway	197:221	the nigrostriatal pathway in the brain	197:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	10	33	from	age-	1754:1757	arg1	proteome					1817:1824	proteome	1817:1824	proteome	1817:1824	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	10	33	from	age-	1754:1757	arg1	receptors					1803:1811	glycan receptors	1796:1811	glycan receptors	1796:1811	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	1	34	theme	progressive	142:152	arg1	loss					154:157	the progressive loss	138:157	the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain	138:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	10	35	from	changes	1785:1791	arg1	proteome					1817:1824	proteome	1817:1824	proteome	1817:1824	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	10	35	from	changes	1785:1791	arg1	receptors					1803:1811	glycan receptors	1796:1811	glycan receptors	1796:1811	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	9	36	from	expression	1672:1681	arg1	pathway					1710:1716	the aging nigrostriatal pathway	1686:1716	the aging nigrostriatal pathway	1686:1716	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	8	37	theme	brain	1472:1476	arg1	region-					1478:1484	brain region- and age-specific N-glycan compositional profiles	1472:1533	region-	1478:1484	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	2	38	theme	neurons	388:394	arg1	degeneration					349:360	the degeneration	345:360	the degeneration of nigrostriatal dopamine neurons in PD patients	345:409	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	5	39	dep	proteoglycans	855:867	arg1	receptor					870:877	receptor	870:877	receptor for rAAV2	870:887	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	3	40	from	models	613:618	arg1	successful					587:596	successful	587:596	successful	587:596	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	3	41	theme	Adeno-associated	412:427	arg1	AAV					438:440	AAV	438:440	AAV	438:440	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	3	41	theme	Adeno-associated	412:427	arg1	vectors					447:453	vectors	447:453	vectors of choice in gene therapy	447:479	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	3	41	theme	Adeno-associated	412:427	arg1	viruses					429:435	Adeno-associated viruses	412:435	Adeno-associated viruses (AAV)	412:441	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	0	42	theme	Glycomic	0:7	arg1	Changes					23:29	Glycomic and Proteomic Changes	0:29	Glycomic and Proteomic Changes in Aging Brain	0:44	Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.					
29915149	8	43	theme	N-glycan	1503:1510	arg1	profiles					1526:1533	brain region- and age-specific N-glycan compositional profiles	1472:1533	profiles	1526:1533	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	0	44	theme	Proteomic	13:21	arg1	Changes					23:29	Glycomic and Proteomic Changes	0:29	Glycomic and Proteomic Changes in Aging Brain	0:44	Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.					
29915149	0	45	dep	Pathway	60:66	arg1	Nigrostriatal					46:58	Nigrostriatal	46:58	Nigrostriatal	46:58	Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.					
29915149	2	46	theme	PD	399:400	arg1	patients					402:409	PD patients	399:409	PD patients	399:409	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	5	47	theme	poor	964:967	arg1	binding					992:998	poor adeno-associated virus binding	964:998	poor adeno-associated virus binding in either the striatum or substantia nigra, or both	964:1050	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	3	48	theme	gene	468:471	arg1	therapy					473:479	gene therapy	468:479	gene therapy	468:479	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	5	49	theme	virus	986:990	arg1	binding					992:998	poor adeno-associated virus binding	964:998	poor adeno-associated virus binding in either the striatum or substantia nigra, or both	964:1050	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	10	50	theme	vectors	1868:1874	arg1	design					1843:1848	design	1843:1848	design of improved viral vectors for Parkinson Disease (PD) gene therapy	1843:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	3	51	theme	choice	458:463	arg1	vectors					447:453	vectors	447:453	vectors of choice in gene therapy	447:479	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	3	51	theme	choice	458:463	arg1	viruses					429:435	Adeno-associated viruses	412:435	Adeno-associated viruses (AAV)	412:441	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	6	52	from	signatures	1207:1216	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	6	52	from	signatures	1207:1216	arg1	nigra					1273:1277	young versus aged rat brain striatum and substantia nigra	1221:1277	nigra	1273:1277	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	8	53	theme	galactose	1564:1572	arg1	receptors					1594:1602	receptors	1594:1602	receptors for AAV9	1594:1611	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	8	53	theme	galactose	1564:1572	arg1	units					1574:1578	the terminal galactose units	1551:1578	the terminal galactose units that serve as receptors for AAV9	1551:1611	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	10	54	theme	improved	1853:1860	arg1	vectors					1868:1874	improved viral vectors	1853:1874	improved viral vectors for Parkinson Disease (PD) gene therapy	1853:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	6	55	theme	young	1221:1225	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	10	56	theme	Parkinson	1880:1888	arg1	PD					1899:1900	PD	1899:1900	PD	1899:1900	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	10	56	theme	Parkinson	1880:1888	arg1	Disease					1890:1896	Parkinson Disease	1880:1896	Parkinson Disease (PD) gene therapy	1880:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	3	57	theme	efficacy	528:535	arg1	profiles					537:544	their well-characterized safety and efficacy profiles	492:544	their well-characterized safety and efficacy profiles	492:544	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	7	58	theme	region-specific	1398:1412	arg1	profiles					1414:1421	brain region-specific profiles	1392:1421	brain region-specific profiles in striatum versus substantia nigra	1392:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	3	59	theme	safety	517:522	arg1	profiles					537:544	their well-characterized safety and efficacy profiles	492:544	their well-characterized safety and efficacy profiles	492:544	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	5	60	theme	sulfate	842:848	arg1	proteoglycans					855:867	heparan sulfate (HS) proteoglycans	834:867	heparan sulfate (HS) proteoglycans (receptor for rAAV2)	834:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	9	61	theme	region-specific	1637:1651	arg1	changes					1653:1659	brain region-specific changes	1631:1659	brain region-specific changes	1631:1659	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	7	62	theme	young	1371:1375	arg1	rats					1383:1386	young adult rats	1371:1386	young adult rats	1371:1386	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	5	63	with	N-glycans	898:906	arg1	galactose					922:930	terminal galactose	913:930	terminal galactose (receptor for rAAV9)	913:951	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	10	64	theme	brain	1763:1767	arg1	changes					1785:1791	brain region-specific changes	1763:1791	brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy	1763:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	1	65	theme	functional	162:171	arg1	neurons					186:192	functional dopaminergic neurons	162:192	functional dopaminergic neurons	162:192	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	2	66	from	degeneration	349:360	arg1	patients					402:409	PD patients	399:409	PD patients	399:409	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	2	67	theme	gene	298:301	arg1	therapy					303:309	gene therapy	298:309	gene therapy	298:309	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	1	68	theme	neurons	186:192	arg1	loss					154:157	the progressive loss	138:157	the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain	138:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	7	69	from	profiles	1414:1421	arg1	striatum					1426:1433	striatum	1426:1433	striatum	1426:1433	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	7	69	from	profiles	1414:1421	arg1	nigra					1453:1457	substantia nigra	1442:1457	substantia nigra	1442:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	10	70	theme	glycan	1796:1801	arg1	receptors					1803:1811	glycan receptors	1796:1811	glycan receptors	1796:1811	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	5	71	theme	glycan	814:819	arg1	receptors					821:829	glycan receptors	814:829	glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2)	814:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	7	72	theme	disaccharide	1327:1338	arg1	profiles					1340:1347	different brain region-specific HS disaccharide profiles	1292:1347	different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra	1292:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	6	73	theme	brain	1243:1247	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	3	74	theme	gene	565:568	arg1	therapy					570:576	gene therapy	565:576	gene therapy	565:576	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	9	75	theme	nigrostriatal	1696:1708	arg1	pathway					1710:1716	the aging nigrostriatal pathway	1686:1716	the aging nigrostriatal pathway	1686:1716	We also observed brain region-specific changes in protein expression in the aging nigrostriatal pathway.					
29915149	7	76	theme	region-specific	1308:1322	arg1	profiles					1340:1347	different brain region-specific HS disaccharide profiles	1292:1347	different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra	1292:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	6	77	theme	aged	1234:1237	arg1	striatum					1249:1256	young versus aged rat brain striatum and substantia nigra	1221:1277	striatum	1249:1256	To test our hypothesis we analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain striatum and substantia nigra.					
29915149	3	78	theme	preclinical	601:611	arg1	models					613:618	preclinical models	601:618	preclinical models of the disease	601:633	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	1	79	theme	neurological	99:110	arg1	disorder					112:119	a neurological disorder	97:119	a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain	97:234	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	1	79	theme	neurological	99:110	arg1	disease					81:87	Parkinson's disease	69:87	Parkinson's disease (PD)	69:92	Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain.					
29915149	7	80	theme	different	1292:1300	arg1	profiles					1340:1347	different brain region-specific HS disaccharide profiles	1292:1347	different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra	1292:1457	We observed different brain region-specific HS disaccharide profiles in aged compared with young adult rats for brain region-specific profiles in striatum versus substantia nigra.					
29915149	5	81	dep	galactose	922:930	arg1	receptor					933:940	receptor	933:940	receptor for rAAV9	933:950	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	0	82	from	Changes	23:29	arg1	Brain					40:44	Aging Brain	34:44	Aging Brain	34:44	Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.					
29915149	5	83	from	binding	992:998	arg1	both					1047:1050	both	1047:1050	both	1047:1050	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	5	83	from	binding	992:998	arg1	striatum					1014:1021	striatum	1014:1021	striatum	1014:1021	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	5	83	from	binding	992:998	arg1	nigra					1037:1041	substantia nigra	1026:1041	substantia nigra	1026:1041	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	5	84	theme	proteoglycans	855:867	arg1	receptors					821:829	glycan receptors	814:829	glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2)	814:888	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	10	85	theme	viral	1862:1866	arg1	vectors					1868:1874	improved viral vectors	1853:1874	improved viral vectors for Parkinson Disease (PD) gene therapy	1853:1914	These studies provide insight into age- and brain region-specific changes in glycan receptors and proteome that will inform design of improved viral vectors for Parkinson Disease (PD) gene therapy.					
29915149	5	86	theme	gene	1080:1083	arg1	delivery					1085:1092	gene delivery	1080:1092	gene delivery	1080:1092	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	5	87	theme	terminal	913:920	arg1	galactose					922:930	terminal galactose	913:930	terminal galactose (receptor for rAAV9)	913:951	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	3	88	from	successful	587:596	arg1	models					613:618	preclinical models	601:618	preclinical models of the disease	601:633	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	8	89	theme	age-specific	1490:1501	arg1	profiles					1526:1533	brain region- and age-specific N-glycan compositional profiles	1472:1533	profiles	1526:1533	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	4	90	theme	virus	747:751	arg1	glycans					757:763	glycans	757:763	glycans	757:763	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	4	90	theme	virus	747:751	arg1	receptors					730:738	all primary AAV receptors	714:738	all primary AAV receptors of the virus	714:751	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	2	91	theme	dopamine	379:386	arg1	neurons					388:394	nigrostriatal dopamine neurons	365:394	nigrostriatal dopamine neurons	365:394	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	8	92	theme	compositional	1512:1524	arg1	profiles					1526:1533	brain region- and age-specific N-glycan compositional profiles	1472:1533	profiles	1526:1533	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	3	93	theme	disease	627:633	arg1	models					613:618	preclinical models	601:618	preclinical models of the disease	601:633	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	4	94	theme	AAV	726:728	arg1	glycans					757:763	glycans	757:763	glycans	757:763	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	4	94	theme	AAV	726:728	arg1	receptors					730:738	all primary AAV receptors	714:738	all primary AAV receptors of the virus	714:751	Significantly, all primary AAV receptors of the virus are glycans.					
29915149	0	95	theme	Aging	34:38	arg1	Brain					40:44	Aging Brain	34:44	Aging Brain	34:44	Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.					
29915149	5	96	theme	adeno-associated	969:984	arg1	binding					992:998	poor adeno-associated virus binding	964:998	poor adeno-associated virus binding in either the striatum or substantia nigra, or both	964:1050	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	8	97	theme	terminal	1555:1562	arg1	receptors					1594:1602	receptors	1594:1602	receptors for AAV9	1594:1611	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	8	97	theme	terminal	1555:1562	arg1	units					1574:1578	the terminal galactose units	1551:1578	the terminal galactose units that serve as receptors for AAV9	1551:1611	We observed brain region- and age-specific N-glycan compositional profiles with respect to the terminal galactose units that serve as receptors for AAV9.					
29915149	3	98	from	vectors	447:453	arg1	therapy					473:479	gene therapy	468:479	gene therapy	468:479	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	2	99	contain	has	311:313	arg1	therapy					303:309	gene therapy	298:309	gene therapy	298:309	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	2	99	contain	has	311:313	arg2	potential					319:327	the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients	315:409	the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients	315:409	Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients.					
29915149	3	100	theme	clinical	636:643	arg1	trials					645:650	clinical trials	636:650	clinical trials in humans	636:660	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	5	101	dep	striatum	1014:1021	arg1	the					1010:1012	the	1010:1012	the	1010:1012	We thus hypothesize that age related changes in glycan receptors of heparan sulfate (HS) proteoglycans (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) results in poor adeno-associated virus binding in either the striatum or substantia nigra, or both, affecting transduction and gene delivery.					
29915149	3	102	from	trials	645:650	arg1	humans					655:660	humans	655:660	humans	655:660	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
29915149	3	103	theme	well-characterized	498:515	arg1	safety					517:522	their well-characterized safety	492:522	their well-characterized safety	492:522	Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene therapy has been successful in preclinical models of the disease, clinical trials in humans have failed to demonstrate efficacy.					
31655162	0	0	theme	insight	121:127	arg1	characterization					12:27	Biochemical characterization	0:27	Biochemical characterization	0:27	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	0	0	theme	insight	121:127	arg1	analysis					44:51	mutational analysis	33:51	mutational analysis	33:51	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	4	1	theme	mori	1065:1068	arg1	N-glycome					1070:1078	the B. mori N-glycome	1058:1078	the B. mori N-glycome	1058:1078	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	1	2	theme	Bombyx	216:221	arg1	mori					223:226	Bombyx mori	216:226	Silkworm Bombyx mori	207:226	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	0	3	dep	mori	72:75	arg1	β-1,4-N-acetylgalactosaminyltransferase					77:115	β-1,4-N-acetylgalactosaminyltransferase	77:115	β-1,4-N-acetylgalactosaminyltransferase	77:115	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	4	4	theme	B.	1062:1063	arg1	N-glycome					1070:1078	the B. mori N-glycome	1058:1078	the B. mori N-glycome	1058:1078	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	3	5	theme	low	753:755	arg1	levels					757:762	very low levels	748:762	very low levels compared with levels of high mannose- and paucimannose-type glycans	748:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	6	6	theme	soluble	1371:1377	arg1	form					1379:1382	a soluble form	1369:1382	a soluble form using a silkworm expression system	1369:1417	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	5	7	contain	contains	1195:1202	arg2	β-1,4-galactosyltransferase					1214:1240	mammalian β-1,4-galactosyltransferase	1204:1240	mammalian β-1,4-galactosyltransferase	1204:1240	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	5	7	contain	contains	1195:1202	arg1	family					1133:1138	the β-1,4-galactosyltransferase family	1101:1138	the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase	1101:1291	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	5	7	contain	contains	1195:1202	arg2	β-1,4-N-acetylgalactosaminyltransferase					1253:1291	insect β-1,4-N-acetylgalactosaminyltransferase	1246:1291	insect β-1,4-N-acetylgalactosaminyltransferase	1246:1291	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	2	8	theme	posttranslational	399:415	arg1	modifications					417:429	proper posttranslational modifications	392:429	proper posttranslational modifications	392:429	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	8	theme	posttranslational	399:415	arg1	N-glycosylation					440:454	N-glycosylation	440:454	N-glycosylation	440:454	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	1	9	theme	industrial	306:315	arg1	levels					317:322	industrial levels	306:322	industrial levels	306:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	6	10	theme	strict	1448:1453	arg1	activity					1495:1502	strict β-1,4-N-acetylgalactosaminyltransferase activity	1448:1502	strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity	1448:1547	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	7	11	theme	substrate	1760:1768	arg1	specificity					1770:1780	substrate specificity	1760:1780	substrate specificity of GT7	1760:1787	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	8	12	contain	have	1942:1945	arg2	gene					1956:1959	an extra gene	1947:1959	an extra gene for a GT7 enzyme with different specificity	1947:2003	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	8	12	contain	have	1942:1945	arg1	mori					1931:1934	B. mori	1928:1934	B. mori	1928:1934	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	2	13	theme	other	337:341	arg1	cells					350:354	other insect cells	337:354	other insect cells	337:354	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	1	14	dep	Silkworm	207:214	arg1	mori					223:226	Bombyx mori	216:226	Silkworm Bombyx mori	207:226	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	2	15	theme	hybrid-	575:581	arg1	glycans					600:606	hybrid- and complex-type glycans	575:606	hybrid- and complex-type glycans	575:606	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	3	16	theme	high	788:791	arg1	glycans					824:830	high mannose- and paucimannose-type glycans	788:830	high mannose- and paucimannose-type glycans	788:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	8	17	theme	different	1983:1991	arg1	specificity					1993:2003	different specificity	1983:2003	different specificity	1983:2003	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	8	18	theme	extra	1950:1954	arg1	gene					1956:1959	an extra gene	1947:1959	an extra gene for a GT7 enzyme with different specificity	1947:2003	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	7	19	from	located	1608:1614	arg1	site					1630:1633	the active site	1619:1633	the active site	1619:1633	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	5	20	dep	family	1133:1138	arg1	known					1146:1150	known	1146:1150	known as glycosyltransferase family 7, GT7	1146:1187	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	3	21	theme	mannose-	793:800	arg1	glycans					824:830	high mannose- and paucimannose-type glycans	788:830	high mannose- and paucimannose-type glycans	788:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	4	22	theme	complex-type	913:924	arg1	N-glycans					926:934	insect complex-type N-glycans	906:934	insect complex-type N-glycans	906:934	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	3	23	from	ones	688:691	arg1	different					663:671	different	663:671	different	663:671	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	0	24	theme	substrate	138:146	arg1	specificity					148:158	the substrate specificity	134:158	the substrate specificity of β-1,4-galactosyltransferase family enzymes	134:204	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	6	25	theme	silkworm	1392:1399	arg1	system					1412:1417	a silkworm expression system	1390:1417	a silkworm expression system	1390:1417	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	4	26	theme	insect	906:911	arg1	N-glycans					926:934	insect complex-type N-glycans	906:934	insect complex-type N-glycans	906:934	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	7	27	located	located	1608:1614	arg2	located					1608:1614	located	1608:1614	located	1608:1614	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	7	27	located	located	1608:1614	arg1	site					1630:1633	the active site	1619:1633	the active site	1619:1633	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	7	27	located	located	1608:1614	arg2	mutation					1552:1559	A mutation	1550:1559	A mutation	1550:1559	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	6	28	theme	β-1,4-N-acetylgalactosaminyltransferase	1455:1493	arg1	activity					1495:1502	strict β-1,4-N-acetylgalactosaminyltransferase activity	1448:1502	strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity	1448:1547	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	4	29	theme	complex-type	955:966	arg1	N-glycans					968:976	complex-type N-glycans	955:976	complex-type N-glycans	955:976	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	0	30	theme	Biochemical	0:10	arg1	characterization					12:27	Biochemical characterization	0:27	Biochemical characterization	0:27	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	3	31	theme	glycans	824:830	arg1	levels					778:783	levels	778:783	levels of high mannose- and paucimannose-type glycans	778:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	4	32	theme	N-glycans	926:934	arg1	biosynthesis					890:901	the biosynthesis	886:901	the biosynthesis of insect complex-type N-glycans	886:934	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	8	33	theme	GT7	1967:1969	arg1	enzyme					1971:1976	a GT7 enzyme	1965:1976	a GT7 enzyme with different specificity	1965:2003	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	7	34	theme	active	1623:1628	arg1	site					1630:1633	the active site	1619:1633	the active site	1619:1633	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	5	35	theme	mammalian	1204:1212	arg1	β-1,4-galactosyltransferase					1214:1240	mammalian β-1,4-galactosyltransferase	1204:1240	mammalian β-1,4-galactosyltransferase	1204:1240	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	7	36	theme	glycosyltransferase	1648:1666	arg1	activity					1668:1675	its glycosyltransferase activity	1644:1675	its glycosyltransferase activity	1644:1675	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	0	37	theme	mutational	33:42	arg1	analysis					44:51	mutational analysis	33:51	mutational analysis	33:51	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	0	38	theme	β-1,4-galactosyltransferase	163:189	arg1	enzymes					198:204	β-1,4-galactosyltransferase family enzymes	163:204	β-1,4-galactosyltransferase family enzymes	163:204	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	6	39	theme	gene	1428:1431	arg1	product					1433:1439	the gene product	1424:1439	the gene product	1424:1439	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	8	40	theme	complex-type	1852:1863	arg1	N-glycans					1865:1873	the complex-type N-glycans	1848:1873	the complex-type N-glycans	1848:1873	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	2	41	theme	N-glycans	479:487	arg1	structures					465:474	the structures	461:474	the structures of N-glycans in B. mori	461:498	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	41	theme	N-glycans	479:487	arg1	mannose-					516:523	mannose-	516:523	mannose-	516:523	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	8	42	theme	known	2024:2028	arg1	glycosyltransferases					2041:2060	the known insect GT7 glycosyltransferases	2020:2060	the known insect GT7 glycosyltransferases	2020:2060	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	2	43	with	proteins	378:385	arg1	modifications					417:429	proper posttranslational modifications	392:429	proper posttranslational modifications	392:429	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	43	with	proteins	378:385	arg1	N-glycosylation					440:454	N-glycosylation	440:454	N-glycosylation	440:454	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	44	theme	insect	343:348	arg1	cells					350:354	other insect cells	337:354	other insect cells	337:354	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	7	45	theme	GT7	1785:1787	arg1	specificity					1770:1780	substrate specificity	1760:1780	substrate specificity of GT7	1760:1787	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	1	46	theme	hosts	249:253	arg1	hosts					249:253	the insect hosts	238:253	the insect hosts for recombinant protein production at academic and industrial levels	238:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	1	46	theme	hosts	249:253	arg1	one					231:233	one	231:233	one	231:233	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	3	47	theme	paucimannose-type	806:822	arg1	glycans					824:830	high mannose- and paucimannose-type glycans	788:830	high mannose- and paucimannose-type glycans	788:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	0	48	theme	enzymes	198:204	arg1	specificity					148:158	the substrate specificity	134:158	the substrate specificity of β-1,4-galactosyltransferase family enzymes	134:204	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	3	49	theme	insect	722:727	arg1	N-glycomes					734:743	some insect cell N-glycomes	717:743	some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans	717:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	8	50	theme	insect	2030:2035	arg1	glycosyltransferases					2041:2060	the known insect GT7 glycosyltransferases	2020:2060	the known insect GT7 glycosyltransferases	2020:2060	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	3	51	from	levels	757:762	arg1	N-glycomes					734:743	some insect cell N-glycomes	717:743	some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans	717:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	0	52	theme	family	191:196	arg1	enzymes					198:204	β-1,4-galactosyltransferase family enzymes	163:204	β-1,4-galactosyltransferase family enzymes	163:204	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	5	53	theme	glycosyltransferase	1155:1173	arg1	GT7					1185:1187	GT7	1185:1187	GT7	1185:1187	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	5	53	theme	glycosyltransferase	1155:1173	arg1	family					1175:1180	glycosyltransferase family 7	1155:1182	glycosyltransferase family 7	1155:1182	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	2	54	theme	complex-type	587:598	arg1	glycans					600:606	hybrid- and complex-type glycans	575:606	hybrid- and complex-type glycans	575:606	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	1	55	theme	recombinant	259:269	arg1	production					279:288	recombinant protein production	259:288	recombinant protein production at academic and industrial levels	259:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	0	56	theme	Bombyx	65:70	arg1	mori					72:75	silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes	56:204	mori	72:75	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	5	57	theme	insect	1246:1251	arg1	β-1,4-N-acetylgalactosaminyltransferase					1253:1291	insect β-1,4-N-acetylgalactosaminyltransferase	1246:1291	insect β-1,4-N-acetylgalactosaminyltransferase	1246:1291	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	6	58	theme	expression	1401:1410	arg1	system					1412:1417	a silkworm expression system	1390:1417	a silkworm expression system	1390:1417	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	2	59	theme	proper	392:397	arg1	modifications					417:429	proper posttranslational modifications	392:429	proper posttranslational modifications	392:429	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	59	theme	proper	392:397	arg1	N-glycosylation					440:454	N-glycosylation	440:454	N-glycosylation	440:454	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	0	60	theme	silkworm	56:63	arg1	mori					72:75	silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes	56:204	mori	72:75	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	7	61	theme	corresponding	1717:1729	arg1	responsible					1744:1754	responsible	1744:1754	responsible	1744:1754	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	7	61	theme	corresponding	1717:1729	arg1	residues					1731:1738	the corresponding residues	1713:1738	the corresponding residues	1713:1738	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	1	62	from	academic	293:300	arg1	production					279:288	recombinant protein production	259:288	recombinant protein production at academic and industrial levels	259:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	5	63	theme	β-1,4-galactosyltransferase	1105:1131	arg1	family					1133:1138	the β-1,4-galactosyltransferase family	1101:1138	the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase	1101:1291	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	7	64	from	site	1630:1633	arg1	mutation					1552:1559	A mutation	1550:1559	A mutation	1550:1559	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	7	64	from	site	1630:1633	arg1	located					1608:1614	located	1608:1614	located	1608:1614	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	8	65	theme	GT7	2037:2039	arg1	glycosyltransferases					2041:2060	the known insect GT7 glycosyltransferases	2020:2060	the known insect GT7 glycosyltransferases	2020:2060	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	8	66	theme	bioinformatics	1890:1903	arg1	analysis					1905:1912	bioinformatics analysis	1890:1912	bioinformatics analysis	1890:1912	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	1	67	theme	protein	271:277	arg1	production					279:288	recombinant protein production	259:288	recombinant protein production at academic and industrial levels	259:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	3	68	theme	cell	729:732	arg1	N-glycomes					734:743	some insect cell N-glycomes	717:743	some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans	717:830	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	8	69	with	enzyme	1971:1976	arg1	specificity					1993:2003	different specificity	1983:2003	different specificity	1983:2003	These results suggested that BmGalNAcT may be involved in the complex-type N-glycans, and moreover, bioinformatics analysis revealed that B. mori might have an extra gene for a GT7 enzyme with different specificity in addition to the known insect GT7 glycosyltransferases.					
31655162	2	70	from	structures	465:474	arg1	mori					495:498	B. mori	492:498	B. mori	492:498	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	2	71	theme	mammalian	368:376	arg1	proteins					378:385	mammalian proteins	368:385	mammalian proteins with proper posttranslational modifications, such as N-glycosylation	368:454	B. mori and other insect cells can produce mammalian proteins with proper posttranslational modifications, such as N-glycosylation, but the structures of N-glycans in B. mori are mainly high mannose- and paucimannose-type, while mammals also produce hybrid- and complex-type glycans.					
31655162	6	72	from	mori	1339:1342	arg1	BmGalNAcT					1320:1328	BmGalNAcT	1320:1328	BmGalNAcT	1320:1328	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	6	72	from	mori	1339:1342	arg1	protein					1311:1317	a GT7 protein	1305:1317	a GT7 protein (BmGalNAcT) from B. mori	1305:1342	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	3	73	theme	complex-type	619:630	arg1	N-glycans					632:640	complex-type N-glycans	619:640	complex-type N-glycans whose structures are different from mammalian ones	619:691	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	6	74	dep	cloned	1348:1353	arg1	expressed					1356:1364	expressed	1356:1364	expressed in a soluble form using a silkworm expression system	1356:1417	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	4	75	theme	N-acetylglucosamine-terminated	990:1019	arg1	glycans					1021:1027	N-acetylglucosamine-terminated glycans	990:1027	N-acetylglucosamine-terminated glycans	990:1027	However, their functions and the enzymes involved in the biosynthesis of insect complex-type N-glycans are not clear, and complex-type N-glycans, except for N-acetylglucosamine-terminated glycans, are still not identified in the B. mori N-glycome.					
31655162	3	76	theme	mammalian	678:686	arg1	ones					688:691	mammalian ones	678:691	mammalian ones	678:691	Recently, complex-type N-glycans whose structures are different from mammalian ones have been identified in some insect cell N-glycomes at very low levels compared with levels of high mannose- and paucimannose-type glycans.					
31655162	1	77	theme	insect	242:247	arg1	hosts					249:253	the insect hosts	238:253	the insect hosts for recombinant protein production at academic and industrial levels	238:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	6	78	theme	β-1,4-galactosyltransferase	1512:1538	arg1	activity					1540:1547	β-1,4-galactosyltransferase activity	1512:1547	strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity	1448:1547	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	5	79	dep	known	1146:1150	arg1	also					1141:1144	also	1141:1144	also	1141:1144	Here, we focused on the β-1,4-galactosyltransferase family (also known as glycosyltransferase family 7, GT7) that contains mammalian β-1,4-galactosyltransferase and insect β-1,4-N-acetylgalactosaminyltransferase.					
31655162	0	80	theme	mori	72:75	arg1	characterization					12:27	Biochemical characterization	0:27	Biochemical characterization	0:27	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	0	80	theme	mori	72:75	arg1	analysis					44:51	mutational analysis	33:51	mutational analysis	33:51	Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes.					
31655162	6	81	theme	GT7	1307:1309	arg1	BmGalNAcT					1320:1328	BmGalNAcT	1320:1328	BmGalNAcT	1320:1328	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	6	81	theme	GT7	1307:1309	arg1	protein					1311:1317	a GT7 protein	1305:1317	a GT7 protein (BmGalNAcT) from B. mori	1305:1342	A gene for a GT7 protein (BmGalNAcT) from B. mori was cloned, expressed in a soluble form using a silkworm expression system, and the gene product showed strict β-1,4-N-acetylgalactosaminyltransferase activity but not β-1,4-galactosyltransferase activity.					
31655162	1	82	from	levels	317:322	arg1	production					279:288	recombinant protein production	259:288	recombinant protein production at academic and industrial levels	259:322	Silkworm Bombyx mori is one of the insect hosts for recombinant protein production at academic and industrial levels.					
31655162	7	83	from	mutation	1552:1559	arg1	Ile310					1574:1579	Ile310	1574:1579	Ile310	1574:1579	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
31655162	7	83	from	mutation	1552:1559	arg1	Ile298					1564:1569	Ile298	1564:1569	Ile298	1564:1569	A mutation in Ile298 or Ile310, which are predicted to be located in the active site, reduced its glycosyltransferase activity, suggesting that these residues and the corresponding residues are responsible for substrate specificity of GT7.					
30389987	1	0	theme	conserved	308:316	arg1	N-X-T/S					325:331	the conserved sequon N-X-T/S	304:331	the conserved sequon N-X-T/S	304:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	4	1	theme	inhibitory	779:788	arg1	peptide					790:796	an inhibitory peptide	776:796	an inhibitory peptide	776:796	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	6	2	theme	reaction	1315:1322	arg1	reaction					1315:1322	the nucleophilic substitution reaction	1285:1322	the nucleophilic substitution reaction	1285:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	6	2	theme	reaction	1315:1322	arg1	group					1276:1280	the leaving group	1264:1280	the leaving group of the nucleophilic substitution reaction	1264:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	8	3	from	relevant	1612:1619	arg1	mechanisms					1638:1647	the catalytic mechanisms	1624:1647	the catalytic mechanisms of all OSTs	1624:1659	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	2	4	theme	LLO	443:445	arg1	analog					447:452	a non-hydrolyzable LLO analog	424:452	a non-hydrolyzable LLO analog	424:452	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	1	5	theme	sequon	318:323	arg1	N-X-T/S					325:331	the conserved sequon N-X-T/S	304:331	the conserved sequon N-X-T/S	304:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	6	link	lipid-linked	236:247	arg1	LLO					266:268	LLO	266:268	LLO	266:268	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	6	link	lipid-linked	236:247	arg1	oligosaccharide					249:263	a lipid-linked oligosaccharide	234:263	a lipid-linked oligosaccharide (LLO)	234:269	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	7	7	theme	reducing-end	1433:1444	arg1	sugar					1446:1450	the reducing-end sugar	1429:1450	the reducing-end sugar of the LLO	1429:1461	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	6	8	theme	substitution	1302:1313	arg1	reaction					1315:1322	the nucleophilic substitution reaction	1285:1322	the nucleophilic substitution reaction	1285:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	5	9	theme	closer	1077:1082	arg1	conformation					1064:1075	a conformation	1062:1075	a conformation closer to the transition state of the reaction	1062:1122	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	10	theme	previous	1009:1016	arg1	structure					1018:1026	the previous structure	1005:1026	the previous structure	1005:1026	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	2	11	theme	substrate-binding	582:598	arg1	contacts					600:607	specific substrate-binding contacts	573:607	specific substrate-binding contacts	573:607	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	6	12	theme	nucleophilic	1289:1300	arg1	reaction					1315:1322	the nucleophilic substitution reaction	1285:1322	the nucleophilic substitution reaction	1285:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	1	13	theme	lipid-linked	236:247	arg1	LLO					266:268	LLO	266:268	LLO	266:268	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	13	theme	lipid-linked	236:247	arg1	oligosaccharide					249:263	a lipid-linked oligosaccharide	234:263	a lipid-linked oligosaccharide (LLO)	234:269	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	2	14	theme	acceptor	470:477	arg1	peptide					479:485	a wild type acceptor peptide	458:485	a wild type acceptor peptide	458:485	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	5	15	theme	peptide	977:983	arg1	group					964:968	the -NH2 group	955:968	the -NH2 group of the peptide	955:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	15	theme	peptide	977:983	arg1	peptide					977:983	the peptide	973:983	the peptide	973:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	15	theme	peptide	977:983	arg1	LLO					947:949	LLO	947:949	LLO	947:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	15	theme	peptide	977:983	arg1	moiety					937:942	the GlcNAc moiety	926:942	the GlcNAc moiety of LLO	926:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	2	16	theme	external	530:537	arg1	EL5					545:547	EL5	545:547	EL5	545:547	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	16	theme	external	530:537	arg1	loop					539:542	an external loop	527:542	an external loop (EL5) of the enzyme	527:562	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	17	theme	specific	573:580	arg1	contacts					600:607	specific substrate-binding contacts	573:607	specific substrate-binding contacts	573:607	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	5	18	theme	N-glycosidic	900:911	arg1	bond					913:916	the N-glycosidic bond	896:916	the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide)	896:984	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	7	19	theme	bound	1521:1525	arg1	ion					1542:1544	a bound divalent metal ion	1519:1544	a bound divalent metal ion	1519:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	20	theme	interaction	1401:1411	arg1	network					1413:1419	an interaction network	1398:1419	an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion	1398:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	2	21	theme	type	465:468	arg1	peptide					479:485	a wild type acceptor peptide	458:485	a wild type acceptor peptide	458:485	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	3	22	theme	substrates	666:675	arg1	separation					648:657	a relatively large separation	629:657	a relatively large separation of the substrates at the active site	629:694	However, there was a relatively large separation of the substrates at the active site.					
30389987	2	23	theme	enzyme	557:562	arg1	EL5					545:547	EL5	545:547	EL5	545:547	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	23	theme	enzyme	557:562	arg1	loop					539:542	an external loop	527:542	an external loop (EL5) of the enzyme	527:562	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	7	24	theme	peptide	1506:1512	arg1	chain					1484:1488	the asparagine side chain	1464:1488	the asparagine side chain of the acceptor peptide	1464:1512	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	24	theme	peptide	1506:1512	arg1	sugar					1446:1450	the reducing-end sugar	1429:1450	the reducing-end sugar of the LLO	1429:1461	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	24	theme	peptide	1506:1512	arg1	ion					1542:1544	a bound divalent metal ion	1519:1544	a bound divalent metal ion	1519:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	25	theme	divalent	1527:1534	arg1	ion					1542:1544	a bound divalent metal ion	1519:1544	a bound divalent metal ion	1519:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	2	26	theme	complex	368:374	arg1	structure					345:353	A previous structure	334:353	A previous structure of a ternary complex of bacterial single subunit OST, PglB,	334:413	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	27	theme	wild	460:463	arg1	type					465:468	a wild type	458:468	a wild type acceptor peptide	458:485	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	6	28	theme	LLO	1211:1213	arg1	ion					1178:1180	the divalent metal ion	1159:1180	the divalent metal ion	1159:1180	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	6	28	theme	LLO	1211:1213	arg1	oxygen					1201:1206	the glycosidic oxygen	1186:1206	the glycosidic oxygen of LLO	1186:1213	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	4	29	theme	reactive	752:759	arg1	analog					765:770	a reactive LLO analog	750:770	a reactive LLO analog	750:770	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	8	30	theme	OSTs	1656:1659	arg1	mechanisms					1638:1647	the catalytic mechanisms	1624:1647	the catalytic mechanisms of all OSTs	1624:1659	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	1	31	theme	N-glycosylation	160:174	arg1	pathway					176:182	the N-glycosylation pathway	156:182	the N-glycosylation pathway	156:182	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	7	32	theme	metal	1536:1540	arg1	ion					1542:1544	a bound divalent metal ion	1519:1544	a bound divalent metal ion	1519:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	1	33	theme	pathway	176:182	arg1	enzyme					146:151	a key enzyme	140:151	a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S	140:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	33	theme	pathway	176:182	arg1	Oligosaccharyltransferase					105:129	Oligosaccharyltransferase	105:129	Oligosaccharyltransferase (OST)	105:135	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	3	34	theme	active	684:689	arg1	site					691:694	the active site	680:694	the active site	680:694	However, there was a relatively large separation of the substrates at the active site.					
30389987	3	35	from	site	691:694	arg1	separation					648:657	a relatively large separation	629:657	a relatively large separation of the substrates at the active site	629:694	However, there was a relatively large separation of the substrates at the active site.					
30389987	7	36	theme	carboxylate	1338:1348	arg1	group					1350:1354	the carboxylate group	1334:1354	the carboxylate group of a conserved aspartate of PglB	1334:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	36	theme	carboxylate	1338:1348	arg1	aspartate					1371:1379	a conserved aspartate	1359:1379	a conserved aspartate of PglB	1359:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	4	37	theme	LLO	761:763	arg1	analog					765:770	a reactive LLO analog	750:770	a reactive LLO analog	750:770	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	6	38	theme	glycosidic	1190:1199	arg1	oxygen					1201:1206	the glycosidic oxygen	1186:1206	the glycosidic oxygen of LLO	1186:1213	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	0	39	theme	oligosaccharyltransferase	23:47	arg1	PglB					49:52	bacterial oligosaccharyltransferase PglB	13:52	bacterial oligosaccharyltransferase PglB	13:52	Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.					
30389987	8	40	theme	catalytic	1628:1636	arg1	mechanisms					1638:1647	the catalytic mechanisms	1624:1647	the catalytic mechanisms of all OSTs	1624:1659	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	2	41	theme	subunit	396:402	arg1	OST					404:406	bacterial single subunit OST	379:406	bacterial single subunit OST	379:406	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	0	42	theme	bacterial	13:21	arg1	PglB					49:52	bacterial oligosaccharyltransferase PglB	13:52	bacterial oligosaccharyltransferase PglB	13:52	Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.					
30389987	7	43	theme	asparagine	1468:1477	arg1	chain					1484:1488	the asparagine side chain	1464:1488	the asparagine side chain of the acceptor peptide	1464:1512	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	1	44	from	oligosaccharide	249:263	arg1	transfer					208:215	the transfer	204:215	the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S	204:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	44	from	oligosaccharide	249:263	arg1	glycan					222:227	a glycan	220:227	a glycan from a lipid-linked oligosaccharide (LLO)	220:269	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	5	45	theme	-NH2	959:962	arg1	group					964:968	the -NH2 group	955:968	the -NH2 group of the peptide	955:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	45	theme	-NH2	959:962	arg1	peptide					977:983	the peptide	973:983	the peptide	973:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	45	theme	-NH2	959:962	arg1	LLO					947:949	LLO	947:949	LLO	947:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	7	46	theme	conserved	1361:1369	arg1	aspartate					1371:1379	a conserved aspartate	1359:1379	a conserved aspartate of PglB	1359:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	2	47	theme	single	389:394	arg1	OST					404:406	bacterial single subunit OST	379:406	bacterial single subunit OST	379:406	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	48	theme	non-hydrolyzable	426:441	arg1	analog					447:452	a non-hydrolyzable LLO analog	424:452	a non-hydrolyzable LLO analog	424:452	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	7	49	theme	side	1479:1482	arg1	chain					1484:1488	the asparagine side chain	1464:1488	the asparagine side chain of the acceptor peptide	1464:1512	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	50	theme	PglB	1384:1387	arg1	aspartate					1371:1379	a conserved aspartate	1359:1379	a conserved aspartate of PglB	1359:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	5	51	theme	group	964:968	arg1	C-1					919:921	C-1	919:921	C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide	919:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	7	52	theme	acceptor	1497:1504	arg1	peptide					1506:1512	the acceptor peptide	1493:1512	the acceptor peptide	1493:1512	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	2	53	theme	bacterial	379:387	arg1	OST					404:406	bacterial single subunit OST	379:406	bacterial single subunit OST	379:406	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	0	54	theme	PglB	49:52	arg1	Structure					0:8	Structure	0:8	Structure of bacterial oligosaccharyltransferase PglB	0:52	Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.					
30389987	4	55	theme	active	851:856	arg1	site					858:861	the active site	847:861	the active site	847:861	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	7	56	theme	aspartate	1371:1379	arg1	group					1350:1354	the carboxylate group	1334:1354	the carboxylate group of a conserved aspartate of PglB	1334:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	56	theme	aspartate	1371:1379	arg1	aspartate					1371:1379	a conserved aspartate	1359:1379	a conserved aspartate of PglB	1359:1387	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	4	57	theme	PglB	736:739	arg1	structure					723:731	the X-ray structure	713:731	the X-ray structure of PglB bound to a reactive LLO analog	713:770	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	4	57	theme	PglB	736:739	arg1	peptide					790:796	an inhibitory peptide	776:796	an inhibitory peptide	776:796	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	5	58	dep	bond	913:916	arg1	C-1					919:921	C-1	919:921	C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide	919:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	1	59	theme	acceptor	277:284	arg1	asparagine					286:295	an acceptor asparagine	274:295	an acceptor asparagine within the conserved sequon N-X-T/S	274:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	5	60	theme	GlcNAc	930:935	arg1	moiety					937:942	the GlcNAc moiety	926:942	the GlcNAc moiety of LLO	926:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	6	61	theme	divalent	1163:1170	arg1	ion					1178:1180	the divalent metal ion	1159:1180	the divalent metal ion	1159:1180	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	6	62	theme	metal	1172:1176	arg1	ion					1178:1180	the divalent metal ion	1159:1180	the divalent metal ion	1159:1180	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	4	63	from	interactions	831:842	arg1	site					858:861	the active site	847:861	the active site	847:861	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	5	64	theme	moiety	937:942	arg1	C-1					919:921	C-1	919:921	C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide	919:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	8	65	from	mechanisms	1638:1647	arg1	likely					1599:1604	likely	1599:1604	likely	1599:1604	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	8	65	from	mechanisms	1638:1647	arg1	relevant					1612:1619	relevant	1612:1619	relevant	1612:1619	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	8	65	from	mechanisms	1638:1647	arg1	interactions					1551:1562	The interactions	1547:1562	The interactions identified in this novel state	1547:1593	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	7	66	theme	LLO	1459:1461	arg1	chain					1484:1488	the asparagine side chain	1464:1488	the asparagine side chain of the acceptor peptide	1464:1512	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	66	theme	LLO	1459:1461	arg1	sugar					1446:1450	the reducing-end sugar	1429:1450	the reducing-end sugar of the LLO	1429:1461	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	7	66	theme	LLO	1459:1461	arg1	ion					1542:1544	a bound divalent metal ion	1519:1544	a bound divalent metal ion	1519:1544	Further, the carboxylate group of a conserved aspartate of PglB mediates an interaction network between the reducing-end sugar of the LLO, the asparagine side chain of the acceptor peptide, and a bound divalent metal ion.					
30389987	1	67	from	transfer	208:215	arg1	LLO					266:268	LLO	266:268	LLO	266:268	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	67	from	transfer	208:215	arg1	oligosaccharide					249:263	a lipid-linked oligosaccharide	234:263	a lipid-linked oligosaccharide (LLO)	234:269	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	5	68	theme	LLO	947:949	arg1	group					964:968	the -NH2 group	955:968	the -NH2 group of the peptide	955:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	68	theme	LLO	947:949	arg1	peptide					977:983	the peptide	973:983	the peptide	973:983	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	68	theme	LLO	947:949	arg1	LLO					947:949	LLO	947:949	LLO	947:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	5	68	theme	LLO	947:949	arg1	moiety					937:942	the GlcNAc moiety	926:942	the GlcNAc moiety of LLO	926:949	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	4	69	theme	X-ray	717:721	arg1	structure					723:731	the X-ray structure	713:731	the X-ray structure of PglB bound to a reactive LLO analog	713:770	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	5	70	theme	transition	1091:1100	arg1	state					1102:1106	the transition state	1087:1106	the transition state of the reaction	1087:1122	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	4	71	theme	unobserved	820:829	arg1	interactions					831:842	previously unobserved interactions	809:842	previously unobserved interactions in the active site	809:861	Here we present the X-ray structure of PglB bound to a reactive LLO analog and an inhibitory peptide, revealing previously unobserved interactions in the active site.					
30389987	2	72	theme	previous	336:343	arg1	structure					345:353	A previous structure	334:353	A previous structure of a ternary complex of bacterial single subunit OST, PglB,	334:413	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	8	73	theme	novel	1583:1587	arg1	state					1589:1593	this novel state	1578:1593	this novel state	1578:1593	The interactions identified in this novel state are likely to be relevant in the catalytic mechanisms of all OSTs.					
30389987	6	74	theme	leaving	1268:1274	arg1	reaction					1315:1322	the nucleophilic substitution reaction	1285:1322	the nucleophilic substitution reaction	1285:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	6	74	theme	leaving	1268:1274	arg1	group					1276:1280	the leaving group	1264:1280	the leaving group of the nucleophilic substitution reaction	1264:1322	We find that the distance between the divalent metal ion and the glycosidic oxygen of LLO is now 4 Å, suggesting that the metal stabilizes the leaving group of the nucleophilic substitution reaction.					
30389987	2	75	dep	showed	487:492	arg1	provided					564:571	provided	564:571	provided specific substrate-binding contacts	564:607	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	75	dep	showed	487:492	arg1	bind					514:517	bind	514:517	bind	514:517	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	76	theme	OST	404:406	arg1	PglB					409:412	PglB	409:412	PglB	409:412	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	76	theme	OST	404:406	arg1	complex					368:374	a ternary complex	358:374	a ternary complex of bacterial single subunit OST	358:406	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	77	theme	ternary	360:366	arg1	PglB					409:412	PglB	409:412	PglB	409:412	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	2	77	theme	ternary	360:366	arg1	complex					368:374	a ternary complex	358:374	a ternary complex of bacterial single subunit OST	358:406	A previous structure of a ternary complex of bacterial single subunit OST, PglB, bound to a non-hydrolyzable LLO analog and a wild type acceptor peptide showed how both substrates bind and how an external loop (EL5) of the enzyme provided specific substrate-binding contacts.					
30389987	0	78	theme	reactive	65:72	arg1	LLO					74:76	a reactive LLO	63:76	a reactive LLO	63:76	Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.					
30389987	1	79	theme	glycan	222:227	arg1	transfer					208:215	the transfer	204:215	the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S	204:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	3	80	theme	large	642:646	arg1	separation					648:657	a relatively large separation	629:657	a relatively large separation of the substrates at the active site	629:694	However, there was a relatively large separation of the substrates at the active site.					
30389987	1	81	theme	key	142:144	arg1	enzyme					146:151	a key enzyme	140:151	a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S	140:331	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	1	81	theme	key	142:144	arg1	Oligosaccharyltransferase					105:129	Oligosaccharyltransferase	105:129	Oligosaccharyltransferase (OST)	105:135	Oligosaccharyltransferase (OST) is a key enzyme of the N-glycosylation pathway, where it catalyzes the transfer of a glycan from a lipid-linked oligosaccharide (LLO) to an acceptor asparagine within the conserved sequon N-X-T/S.					
30389987	5	82	theme	reaction	1115:1122	arg1	state					1102:1106	the transition state	1087:1106	the transition state of the reaction	1087:1122	We found that the atoms forming the N-glycosidic bond (C-1 of the GlcNAc moiety of LLO and the -NH2 group of the peptide) are closer than in the previous structure, suggesting that we have captured a conformation closer to the transition state of the reaction.					
30389987	0	83	theme	inhibitory	85:94	arg1	peptide					96:102	an inhibitory peptide	82:102	an inhibitory peptide	82:102	Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.					
31276882	2	0	theme	orthogonal	385:394	arg1	activation					412:421	the orthogonal and consecutive activation	381:421	the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates	381:475	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	1	1	theme	structure-activity	121:138	arg1	relationships					140:152	the structure-activity relationships	117:152	the structure-activity relationships of β-(1,3)-glucans	117:171	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	0	2	theme	hexadecasaccharide	47:64	arg1	synthesis					17:25	Gold(I)-promoted synthesis	0:25	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.	0:100	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	0	3	theme	-glucan	39:45	arg1	hexadecasaccharide					47:64	a β-(1,3)-glucan hexadecasaccharide	30:64	a β-(1,3)-glucan hexadecasaccharide	30:64	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	1	4	theme	β-	218:219	arg1	polysaccharides					233:247	the β-(1,3)-glucan polysaccharides	214:247	the β-(1,3)-glucan polysaccharides	214:247	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	2	5	theme	thioglycosides	426:439	arg1	activation					412:421	the orthogonal and consecutive activation	381:421	the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates	381:475	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	1	6	theme	relationships	140:152	arg1	Elucidation					102:112	Elucidation	102:112	Elucidation of the structure-activity relationships of β-(1,3)-glucans	102:171	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	0	7	theme	1,3	35:37	arg1	hexadecasaccharide					47:64	a β-(1,3)-glucan hexadecasaccharide	30:64	a β-(1,3)-glucan hexadecasaccharide	30:64	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	2	8	theme	1,3	346:348	arg1	hexadecasaccharide					358:375	a β-(1,3)-glucan hexadecasaccharide	341:375	a β-(1,3)-glucan hexadecasaccharide	341:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	2	9	theme	-promoted	292:300	arg1	approach					302:309	a gold(I)-promoted approach	283:309	a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide	283:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	0	10	theme	convergent	81:90	arg1	strategy					92:99	the highly convergent strategy	70:99	the highly convergent strategy	70:99	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	2	11	theme	β-	343:344	arg1	hexadecasaccharide					358:375	a β-(1,3)-glucan hexadecasaccharide	341:375	a β-(1,3)-glucan hexadecasaccharide	341:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	1	12	theme	-glucan	225:231	arg1	polysaccharides					233:247	the β-(1,3)-glucan polysaccharides	214:247	the β-(1,3)-glucan polysaccharides	214:247	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	3	13	theme	biological	593:602	arg1	functions					604:612	their biological functions	587:612	their biological functions	587:612	The synthetic hexadecasaccharide serves as the basis for further evaluation of their biological functions.					
31276882	1	14	theme	-glucans	164:171	arg1	relationships					140:152	the structure-activity relationships	117:152	the structure-activity relationships of β-(1,3)-glucans	117:171	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	0	15	theme	-promoted	7:15	arg1	synthesis					17:25	Gold(I)-promoted synthesis	0:25	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.	0:100	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	2	16	theme	ortho-hexynylbenzoates	454:475	arg1	activation					412:421	the orthogonal and consecutive activation	381:421	the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates	381:475	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	2	17	theme	efficient	319:327	arg1	assembly					329:336	the efficient assembly	315:336	the efficient assembly of a β-(1,3)-glucan hexadecasaccharide	315:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	2	18	theme	hexadecasaccharide	358:375	arg1	assembly					329:336	the efficient assembly	315:336	the efficient assembly of a β-(1,3)-glucan hexadecasaccharide	315:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	2	19	theme	glucosyl	445:452	arg1	ortho-hexynylbenzoates					454:475	glucosyl ortho-hexynylbenzoates	445:475	glucosyl ortho-hexynylbenzoates	445:475	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	0	20	theme	β-	32:33	arg1	hexadecasaccharide					47:64	a β-(1,3)-glucan hexadecasaccharide	30:64	a β-(1,3)-glucan hexadecasaccharide	30:64	Gold(I)-promoted synthesis of a β-(1,3)-glucan hexadecasaccharide via the highly convergent strategy.					
31276882	3	21	theme	synthetic	512:520	arg1	basis					555:559	the basis	551:559	the basis for further evaluation of their biological functions	551:612	The synthetic hexadecasaccharide serves as the basis for further evaluation of their biological functions.					
31276882	3	21	theme	synthetic	512:520	arg1	hexadecasaccharide					522:539	The synthetic hexadecasaccharide	508:539	The synthetic hexadecasaccharide	508:539	The synthetic hexadecasaccharide serves as the basis for further evaluation of their biological functions.					
31276882	2	22	theme	-glucan	350:356	arg1	hexadecasaccharide					358:375	a β-(1,3)-glucan hexadecasaccharide	341:375	a β-(1,3)-glucan hexadecasaccharide	341:375	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	1	23	theme	natural	254:260	arg1	sources					262:268	natural sources	254:268	natural sources	254:268	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	1	24	attach	isolate	206:212	arg2	difficulty					192:201	the difficulty	188:201	the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources	188:268	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	1	24	attach	isolate	206:212	arg1	sources					262:268	natural sources	254:268	natural sources	254:268	Elucidation of the structure-activity relationships of β-(1,3)-glucans is hampered by the difficulty to isolate the β-(1,3)-glucan polysaccharides from natural sources.					
31276882	2	25	theme	convergent	489:498	arg1	manner					500:505	a highly convergent manner	480:505	a highly convergent manner	480:505	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
31276882	3	26	theme	functions	604:612	arg1	evaluation					573:582	further evaluation	565:582	further evaluation of their biological functions	565:612	The synthetic hexadecasaccharide serves as the basis for further evaluation of their biological functions.					
31276882	3	27	theme	further	565:571	arg1	evaluation					573:582	further evaluation	565:582	further evaluation of their biological functions	565:612	The synthetic hexadecasaccharide serves as the basis for further evaluation of their biological functions.					
31276882	2	28	theme	consecutive	400:410	arg1	activation					412:421	the orthogonal and consecutive activation	381:421	the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates	381:475	We describe a gold(I)-promoted approach for the efficient assembly of a β-(1,3)-glucan hexadecasaccharide via the orthogonal and consecutive activation of thioglycosides and glucosyl ortho-hexynylbenzoates in a highly convergent manner.					
30628786	0	0	theme	Sepioloidea	71:81	arg1	lineolata					83:91	Sepioloidea lineolata	71:91	Sepioloidea lineolata	71:91	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	1	from	Analysis	22:29	arg1	Squid					122:126	the Southern Bottletail Squid	98:126	the Southern Bottletail Squid	98:126	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	1	from	Analysis	22:29	arg1	Sepiadarium					129:139	Sepiadarium	129:139	Sepiadarium	129:139	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	1	from	Analysis	22:29	arg1	Squid					64:68	the Striped Pyjama Squid	45:68	the Striped Pyjama Squid	45:68	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	1	from	Analysis	22:29	arg1	lineolata					83:91	Sepioloidea lineolata	71:91	Sepioloidea lineolata	71:91	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	1	2	theme	family	230:235	arg1	squid					223:227	the striped pyjama squid	204:227	the striped pyjama squid (family Sepiadariidae)	204:250	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	2	theme	family	230:235	arg1	Sepiadariidae					237:249	family Sepiadariidae	230:249	family Sepiadariidae	230:249	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	7	3	with	cleavage	1293:1300	arg1	PNGase-F					1333:1340	PNGase-F	1333:1340	PNGase-F	1333:1340	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	7	3	with	cleavage	1293:1300	arg1	F					1330:1330	Peptide: N-glycosidase F	1307:1330	Peptide: N-glycosidase F (PNGase-F)	1307:1341	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	6	4	from	gels	1140:1143	arg1	distinct					1106:1113	distinct	1106:1113	distinct	1106:1113	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	1	5	theme	Southern	319:326	arg1	coast					341:345	the Southern Indo-Pacific coast	315:345	the Southern Indo-Pacific coast of Australia	315:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	8	6	theme	strong	1385:1390	arg1	evidence					1392:1399	strong evidence	1385:1399	strong evidence of N-glycosylation	1385:1418	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	5	7	with	proteins	956:963	arg1	homologues					973:982	no homologues	970:982	no homologues outside the class Cephalopoda	970:1012	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	1	8	theme	Indo-Pacific	328:339	arg1	coast					341:345	the Southern Indo-Pacific coast	315:345	the Southern Indo-Pacific coast of Australia	315:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	5	9	theme	abundant	896:903	arg1	proteins					905:912	abundant proteins	896:912	abundant proteins	896:912	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	6	10	theme	polymer-based	1126:1138	arg1	gels					1140:1143	mucin polymer-based gels	1120:1143	mucin polymer-based gels found in many vertebrate and echinoderm secretions	1120:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	4	11	theme	lineolata	714:722	arg1	slime					724:728	Sepioloidea lineolata slime	702:728	Sepioloidea lineolata slime	702:728	Of the 550 protein groups identified in Sepioloidea lineolata slime, 321 had orthologs in Sepiadarium austrinum, and the abundance of these (iBAQ) was highly correlated between species.					
30628786	7	12	theme	N-glycosidase	1316:1328	arg1	PNGase-F					1333:1340	PNGase-F	1333:1340	PNGase-F	1333:1340	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	7	12	theme	N-glycosidase	1316:1328	arg1	F					1330:1330	Peptide: N-glycosidase F	1307:1330	Peptide: N-glycosidase F (PNGase-F)	1307:1341	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	5	13	theme	proteins	905:912	arg1	number					886:891	a small number	878:891	a small number of abundant proteins	878:912	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	2	14	theme	sepiadariid	372:382	arg1	squids					384:389	other sepiadariid squids	366:389	other sepiadariid squids	366:389	Like other sepiadariid squids, it is known to secrete large volumes of viscous slime when stressed.					
30628786	8	15	from	increase	1475:1482	arg1	identification					1495:1508	peptide identification	1487:1508	peptide identification	1487:1508	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	0	16	from	Squid	122:126	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	16	from	Squid	122:126	arg1	Slime					34:38	Slime	34:38	Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae)	34:178	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	2	17	theme	other	366:370	arg1	squids					384:389	other sepiadariid squids	366:389	other sepiadariid squids	366:389	Like other sepiadariid squids, it is known to secrete large volumes of viscous slime when stressed.					
30628786	0	18	theme	Proteomic	12:20	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	1	19	theme	Australia	350:358	arg1	coast					341:345	the Southern Indo-Pacific coast	315:345	the Southern Indo-Pacific coast of Australia	315:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	6	20	theme	echinoderm	1174:1183	arg1	secretions					1185:1194	many vertebrate and echinoderm secretions	1154:1194	many vertebrate and echinoderm secretions	1154:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	7	21	from	extent	1201:1206	arg1	slime					1234:1238	the slime	1230:1238	the slime of Sepioloidea lineolata	1230:1263	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	3	22	theme	sepiadariid	523:533	arg1	slimes					535:540	sepiadariid slimes	523:540	sepiadariid slimes	523:540	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	7	23	theme	Sepioloidea	1243:1253	arg1	lineolata					1255:1263	Sepioloidea lineolata	1243:1263	Sepioloidea lineolata	1243:1263	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	0	24	theme	Comparative	0:10	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	4	25	theme	these	796:800	arg1	abundance					783:791	the abundance	779:791	the abundance of these (iBAQ)	779:807	Of the 550 protein groups identified in Sepioloidea lineolata slime, 321 had orthologs in Sepiadarium austrinum, and the abundance of these (iBAQ) was highly correlated between species.					
30628786	3	26	theme	slimes	535:540	arg1	function					511:518	the function	507:518	the function of sepiadariid slimes	507:540	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	7	27	theme	lineolata	1255:1263	arg1	slime					1234:1238	the slime	1230:1238	the slime of Sepioloidea lineolata	1230:1263	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	6	28	theme	mucin	1120:1124	arg1	gels					1140:1143	mucin polymer-based gels	1120:1143	mucin polymer-based gels found in many vertebrate and echinoderm secretions	1120:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	0	29	theme	Bottletail	111:120	arg1	Squid					122:126	the Southern Bottletail Squid	98:126	the Southern Bottletail Squid	98:126	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	29	theme	Bottletail	111:120	arg1	Sepiadarium					129:139	Sepiadarium	129:139	Sepiadarium	129:139	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	8	30	dep	led	1459:1461	arg1	compared					1516:1523	compared	1516:1523	compared with controls	1516:1537	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	4	31	theme	Sepioloidea	702:712	arg1	slime					724:728	Sepioloidea lineolata slime	702:728	Sepioloidea lineolata slime	702:728	Of the 550 protein groups identified in Sepioloidea lineolata slime, 321 had orthologs in Sepiadarium austrinum, and the abundance of these (iBAQ) was highly correlated between species.					
30628786	1	32	theme	Sepioloidea	181:191	arg1	squid					223:227	the striped pyjama squid	204:227	the striped pyjama squid (family Sepiadariidae)	204:250	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	32	theme	Sepioloidea	181:191	arg1	lineolata					193:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	32	theme	Sepioloidea	181:191	arg1	species					264:270	a small species	256:270	a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia	256:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	3	33	theme	key	482:484	arg1	proteins					486:493	key proteins	482:493	key proteins involved in the function of sepiadariid slimes	482:540	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	1	34	theme	small	258:262	arg1	lineolata					193:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	34	theme	small	258:262	arg1	species					264:270	a small species	256:270	a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia	256:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	0	35	theme	Southern	102:109	arg1	Squid					122:126	the Southern Bottletail Squid	98:126	the Southern Bottletail Squid	98:126	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	35	theme	Southern	102:109	arg1	Sepiadarium					129:139	Sepiadarium	129:139	Sepiadarium	129:139	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	8	36	theme	slight	1468:1473	arg1	increase					1475:1482	a slight increase	1466:1482	a slight increase in peptide identification	1466:1508	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	8	37	theme	few	1358:1360	arg1	proteins					1369:1376	very few (four) proteins	1353:1376	very few (four) proteins	1353:1376	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	2	38	theme	slime	440:444	arg1	volumes					421:427	large volumes	415:427	large volumes of viscous slime	415:444	Like other sepiadariid squids, it is known to secrete large volumes of viscous slime when stressed.					
30628786	7	39	theme	N-glycosylation	1211:1225	arg1	extent					1201:1206	The extent	1197:1206	The extent of N-glycosylation in the slime of Sepioloidea lineolata	1197:1263	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	6	40	theme	vertebrate	1159:1168	arg1	secretions					1185:1194	many vertebrate and echinoderm secretions	1154:1194	many vertebrate and echinoderm secretions	1154:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	8	41	with	treatment	1435:1443	arg1	PNGase-F					1450:1457	PNGase-F	1450:1457	PNGase-F	1450:1457	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	0	42	theme	Slime	34:38	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	2	43	theme	viscous	432:438	arg1	slime					440:444	viscous slime	432:444	viscous slime	432:444	Like other sepiadariid squids, it is known to secrete large volumes of viscous slime when stressed.					
30628786	0	44	from	Squid	64:68	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	44	from	Squid	64:68	arg1	Slime					34:38	Slime	34:38	Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae)	34:178	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	5	45	theme	short	941:945	arg1	proteins					956:963	short secreted proteins	941:963	short secreted proteins with no homologues outside the class Cephalopoda	941:1012	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	8	46	theme	peptide	1487:1493	arg1	identification					1495:1508	peptide identification	1487:1508	peptide identification	1487:1508	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	0	47	theme	Striped	49:55	arg1	Squid					64:68	the Striped Pyjama Squid	45:68	the Striped Pyjama Squid	45:68	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	1	48	theme	benthic	275:281	arg1	squid					291:295	benthic bobtail squid	275:295	benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia	275:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	5	49	theme	secreted	947:954	arg1	proteins					956:963	short secreted proteins	941:963	short secreted proteins with no homologues outside the class Cephalopoda	941:1012	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	0	50	dep	Cephalopoda	152:162	arg1	Sepiadariidae					165:177	Sepiadariidae	165:177	Sepiadariidae	165:177	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	1	51	theme	bobtail	283:289	arg1	squid					291:295	benthic bobtail squid	275:295	benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia	275:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	3	52	theme	related	622:628	arg1	species					630:636	a closely related species	612:636	a closely related species	612:636	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	7	53	theme	Peptide	1307:1313	arg1	PNGase-F					1333:1340	PNGase-F	1333:1340	PNGase-F	1333:1340	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	7	53	theme	Peptide	1307:1313	arg1	F					1330:1330	Peptide: N-glycosidase F	1307:1330	Peptide: N-glycosidase F (PNGase-F)	1307:1341	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	3	54	theme	lineolata	589:597	arg1	proteome					565:572	the slime proteome	555:572	the slime proteome of Sepioloidea lineolata	555:597	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	1	55	theme	squid	291:295	arg1	lineolata					193:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	55	theme	squid	291:295	arg1	species					264:270	a small species	256:270	a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia	256:358	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	7	56	theme	glycan	1286:1291	arg1	cleavage					1293:1300	glycan cleavage	1286:1300	glycan cleavage with Peptide: N-glycosidase F (PNGase-F)	1286:1341	The extent of N-glycosylation in the slime of Sepioloidea lineolata was also studied via glycan cleavage with Peptide: N-glycosidase F (PNGase-F).					
30628786	6	57	theme	many	1154:1157	arg1	secretions					1185:1194	many vertebrate and echinoderm secretions	1154:1194	many vertebrate and echinoderm secretions	1154:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	4	58	theme	protein	673:679	arg1	groups					681:686	the 550 protein groups	665:686	the 550 protein groups identified in Sepioloidea lineolata slime	665:728	Of the 550 protein groups identified in Sepioloidea lineolata slime, 321 had orthologs in Sepiadarium austrinum, and the abundance of these (iBAQ) was highly correlated between species.					
30628786	5	59	theme	small	880:884	arg1	number					886:891	a small number	878:891	a small number of abundant proteins	878:912	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	1	60	theme	striped	208:214	arg1	squid					223:227	the striped pyjama squid	204:227	the striped pyjama squid (family Sepiadariidae)	204:250	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	60	theme	striped	208:214	arg1	Sepiadariidae					237:249	family Sepiadariidae	230:249	family Sepiadariidae	230:249	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	60	theme	striped	208:214	arg1	lineolata					193:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	3	61	theme	Sepioloidea	577:587	arg1	lineolata					589:597	Sepioloidea lineolata	577:597	Sepioloidea lineolata	577:597	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	8	62	theme	N-glycosylation	1404:1418	arg1	evidence					1392:1399	strong evidence	1385:1399	strong evidence of N-glycosylation	1385:1418	Although very few (four) proteins showed strong evidence of N-glycosylation, we found that treatment with PNGase-F led to a slight increase in peptide identification rates compared with controls.					
30628786	0	63	dep	Sepiadarium	129:139	arg1	austrinum					141:149	Sepiadarium austrinum	129:149	Sepiadarium austrinum (Cephalopoda: Sepiadariidae)	129:178	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	63	dep	Sepiadarium	129:139	arg1	Cephalopoda					152:162	Cephalopoda	152:162	Cephalopoda	152:162	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	1	64	theme	pyjama	216:221	arg1	squid					223:227	the striped pyjama squid	204:227	the striped pyjama squid (family Sepiadariidae)	204:250	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	64	theme	pyjama	216:221	arg1	Sepiadariidae					237:249	family Sepiadariidae	230:249	family Sepiadariidae	230:249	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	1	64	theme	pyjama	216:221	arg1	lineolata					193:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata	181:201	Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic bobtail squid distributed along the Southern Indo-Pacific coast of Australia.					
30628786	0	65	from	lineolata	83:91	arg1	Analysis					22:29	Comparative Proteomic Analysis	0:29	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).	0:179	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	0	65	from	lineolata	83:91	arg1	Slime					34:38	Slime	34:38	Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae)	34:178	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	3	66	theme	slime	559:563	arg1	proteome					565:572	the slime proteome	555:572	the slime proteome of Sepioloidea lineolata	555:597	In order to identify key proteins involved in the function of sepiadariid slimes, we compared the slime proteome of Sepioloidea lineolata with that of a closely related species, Sepiadarium austrinum.					
30628786	0	67	theme	Pyjama	57:62	arg1	Squid					64:68	the Striped Pyjama Squid	45:68	the Striped Pyjama Squid	45:68	Comparative Proteomic Analysis of Slime from the Striped Pyjama Squid, Sepioloidea lineolata, and the Southern Bottletail Squid, Sepiadarium austrinum (Cephalopoda: Sepiadariidae).					
30628786	5	68	theme	these	930:934	arg1	several					919:925	several	919:925	several	919:925	Both slimes were dominated by a small number of abundant proteins, and several of these were short secreted proteins with no homologues outside the class Cephalopoda.					
30628786	6	69	located	found	1145:1149	arg1	secretions					1185:1194	many vertebrate and echinoderm secretions	1154:1194	many vertebrate and echinoderm secretions	1154:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	6	69	located	found	1145:1149	arg2	gels					1140:1143	mucin polymer-based gels	1120:1143	mucin polymer-based gels found in many vertebrate and echinoderm secretions	1120:1194	No mucins were identified within either species' slime, suggesting that it is structurally distinct from mucin polymer-based gels found in many vertebrate and echinoderm secretions.					
30628786	2	70	theme	large	415:419	arg1	volumes					421:427	large volumes	415:427	large volumes of viscous slime	415:444	Like other sepiadariid squids, it is known to secrete large volumes of viscous slime when stressed.					
31154130	2	0	theme	significant	441:451	arg1	glycoprotein					453:464	the most dominant and significant glycoprotein	419:464	the most dominant and significant glycoprotein in human serum	419:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	6	1	theme	ultra	1042:1046	arg1	UHPLC					1088:1092	UHPLC	1088:1092	UHPLC	1088:1092	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	6	1	theme	ultra	1042:1046	arg1	chromatography					1072:1085	ultra high performance liquid chromatography	1042:1085	ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS	1042:1113	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	7	2	theme	IgG-Fc	1132:1137	arg1	result					1121:1126	a result	1119:1126	a result	1119:1126	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	2	theme	IgG-Fc	1132:1137	arg1	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	2	theme	IgG-Fc	1132:1137	arg1	glycopeptides					1139:1151	83 IgG-Fc glycopeptides	1129:1151	83 IgG-Fc glycopeptides	1129:1151	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	6	3	gly	glycopeptides	953:965	arg2	glycopeptides					953:965	subclass-specific glycopeptides	935:965	subclass-specific glycopeptides	935:965	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	4	4	gly	glycopeptides	690:702	arg2	glycopeptides					690:702	IgG-Fc glycopeptides	683:702	IgG-Fc glycopeptides	683:702	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	0	5	theme	glycopeptides	78:90	arg1	profiling					41:49	comprehensive profiling	27:49	comprehensive profiling	27:49	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	0	5	theme	glycopeptides	78:90	arg1	quantitation					55:66	quantitation	55:66	quantitation	55:66	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	7	6	from	serum	1164:1168	arg1	result					1121:1126	a result	1119:1126	a result	1119:1126	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	6	from	serum	1164:1168	arg1	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	6	from	serum	1164:1168	arg1	glycopeptides					1139:1151	83 IgG-Fc glycopeptides	1129:1151	83 IgG-Fc glycopeptides	1129:1151	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	3	7	gly	glycosylation	486:498	arg1	moiety					510:515	IgG-Fc moiety	503:515	IgG-Fc moiety	503:515	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	2	8	theme	human	469:473	arg1	serum					475:479	human serum	469:479	human serum	469:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	7	9	gly	sialylated	1184:1193	arg1	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	8	10	theme	glycosylation	1428:1440	arg1	analysis					1412:1419	quantitative analysis	1399:1419	quantitative analysis of IgG glycosylation	1399:1440	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	2	11	theme	Human	313:317	arg1	G					334:334	Human immunoglobulin G	313:334	Human immunoglobulin G (IgG)	313:340	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	2	11	theme	Human	313:317	arg1	IgG					337:339	IgG	337:339	IgG	337:339	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	6	12	theme	MS/MS	1022:1026	arg1	data					1028:1031	high-resolution MS and MS/MS data	999:1031	data	1028:1031	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	5	13	theme	IgG	714:716	arg1	N-glycans					718:726	IgG N-glycans	714:726	IgG N-glycans	714:726	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	9	14	theme	potential	1470:1478	arg1	species					1567:1573	11 species	1564:1573	11 species from IgG4	1564:1583	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	14	theme	potential	1470:1478	arg1	species					1540:1546	12 species	1537:1546	12 species from IgG2/3	1537:1558	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	14	theme	potential	1470:1478	arg1	species					1518:1524	13 species	1515:1524	13 species from IgG1	1515:1534	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	14	theme	potential	1470:1478	arg1	biomarkers					1493:1502	36 potential glycopeptide biomarkers	1467:1502	36 potential glycopeptide biomarkers	1467:1502	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	0	15	gly	glycopeptides	78:90	arg2	glycopeptides					78:90	IgG-Fc glycopeptides	71:90	IgG-Fc glycopeptides	71:90	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	8	16	theme	high	1267:1270	arg1	sensitivity					1272:1282	high sensitivity	1267:1282	high sensitivity	1267:1282	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	5	17	theme	87 N‑glycans	852:863	arg1	characterization					832:847	characterization	832:847	characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions	832:922	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	0	18	theme	rheumatoid	112:121	arg1	arthritis					123:131	rheumatoid arthritis	112:131	rheumatoid arthritis	112:131	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	5	19	theme	chip	761:764	arg1	Q-TOF-MS					811:818	Q-TOF-MS	811:818	Q-TOF-MS	811:818	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	19	theme	chip	761:764	arg1	spectrometry					797:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry	751:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS)	751:819	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	4	20	theme	integrated	615:624	arg1	approach					626:633	an integrated approach	612:633	an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides	612:702	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	0	21	theme	integrated	3:12	arg1	approach					14:21	An integrated approach	0:21	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis	0:131	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	5	22	theme	mAb-Glyco	751:759	arg1	Q-TOF-MS					811:818	Q-TOF-MS	811:818	Q-TOF-MS	811:818	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	22	theme	mAb-Glyco	751:759	arg1	spectrometry					797:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry	751:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS)	751:819	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	1	23	gly	glycoproteins	224:236	arg1	glycoproteins					224:236	glycoproteins	224:236	glycoproteins	224:236	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	6	24	theme	MS	1015:1016	arg1	basis					990:994	the basis	986:994	the basis of high-resolution MS and MS/MS data	986:1031	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	2	25	gly	glycoprotein	453:464	arg1	glycoprotein					453:464	the most dominant and significant glycoprotein	419:464	the most dominant and significant glycoprotein in human serum	419:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	7	26	theme	sialylated	1184:1193	arg1	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	4	27	theme	comprehensive	639:651	arg1	profiling					653:661	comprehensive profiling	639:661	comprehensive profiling	639:661	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	8	28	with	method	1255:1260	arg1	repeatability					1288:1300	repeatability	1288:1300	repeatability	1288:1300	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	8	28	with	method	1255:1260	arg1	sensitivity					1272:1282	high sensitivity	1267:1282	high sensitivity	1267:1282	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	1	29	theme	various	295:301	arg1	diseases					303:310	various diseases	295:310	various diseases	295:310	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	9	30	gly	glycopeptide	1480:1491	arg2	glycopeptide					1480:1491	36 potential glycopeptide biomarkers	1467:1502	36 potential glycopeptide biomarkers	1467:1502	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	8	31	theme	IgG	1424:1426	arg1	glycosylation					1428:1440	IgG glycosylation	1424:1440	IgG glycosylation	1424:1440	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	1	32	theme	structure	198:206	arg1	maintenance					179:189	the maintenance	175:189	the maintenance of the structure and function of glycoproteins	175:236	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	6	33	theme	high-resolution	999:1013	arg1	MS					1015:1016	high-resolution MS and MS/MS data	999:1031	MS	1015:1016	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	9	34	from	IgG1	1531:1534	arg1	species					1567:1573	11 species	1564:1573	11 species from IgG4	1564:1583	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	34	from	IgG1	1531:1534	arg1	species					1540:1546	12 species	1537:1546	12 species from IgG2/3	1537:1558	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	34	from	IgG1	1531:1534	arg1	species					1518:1524	13 species	1515:1524	13 species from IgG1	1515:1534	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	8	35	theme	quantitative	1399:1410	arg1	analysis					1412:1419	quantitative analysis	1399:1419	quantitative analysis of IgG glycosylation	1399:1440	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	7	36	gly	glycopeptides	1139:1151	arg2	glycopeptides					1139:1151	83 IgG-Fc glycopeptides	1129:1151	83 IgG-Fc glycopeptides	1129:1151	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	36	gly	glycopeptides	1139:1151	arg1	serum					1164:1168	human serum	1158:1168	human serum	1158:1168	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	36	gly	glycopeptides	1139:1151	arg2	result					1121:1126	a result	1119:1126	a result	1119:1126	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	36	gly	glycopeptides	1139:1151	arg2	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	2	37	dep	subclasses	369:378	arg1	IgG1					381:384	IgG1	381:384	IgG1	381:384	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	2	38	theme	immunoglobulin	319:332	arg1	G					334:334	Human immunoglobulin G	313:334	Human immunoglobulin G (IgG)	313:340	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	2	38	theme	immunoglobulin	319:332	arg1	IgG					337:339	IgG	337:339	IgG	337:339	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	6	39	theme	subclass-specific	935:951	arg1	glycopeptides					953:965	subclass-specific glycopeptides	935:965	subclass-specific glycopeptides	935:965	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	1	40	theme	function	212:219	arg1	maintenance					179:189	the maintenance	175:189	the maintenance of the structure and function of glycoproteins	175:236	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	2	41	theme	dominant	428:435	arg1	glycoprotein					453:464	the most dominant and significant glycoprotein	419:464	the most dominant and significant glycoprotein in human serum	419:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	3	42	theme	various	548:554	arg1	states					587:592	various physiological and pathological states	548:592	various physiological and pathological states	548:592	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	9	43	theme	glycopeptide	1480:1491	arg1	species					1567:1573	11 species	1564:1573	11 species from IgG4	1564:1583	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	43	theme	glycopeptide	1480:1491	arg1	species					1540:1546	12 species	1537:1546	12 species from IgG2/3	1537:1558	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	43	theme	glycopeptide	1480:1491	arg1	species					1518:1524	13 species	1515:1524	13 species from IgG1	1515:1534	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	43	theme	glycopeptide	1480:1491	arg1	biomarkers					1493:1502	36 potential glycopeptide biomarkers	1467:1502	36 potential glycopeptide biomarkers	1467:1502	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	0	44	theme	comprehensive	27:39	arg1	profiling					41:49	comprehensive profiling	27:49	comprehensive profiling	27:49	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	5	45	theme	quadrupole	766:775	arg1	Q-TOF-MS					811:818	Q-TOF-MS	811:818	Q-TOF-MS	811:818	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	45	theme	quadrupole	766:775	arg1	spectrometry					797:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry	751:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS)	751:819	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	6	46	theme	data	1028:1031	arg1	basis					990:994	the basis	986:994	the basis of high-resolution MS and MS/MS data	986:1031	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	1	47	dep	structure	198:206	arg1	the					194:196	the	194:196	the	194:196	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	1	48	theme	glycoproteins	224:236	arg1	function					212:219	function	212:219	function	212:219	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	1	48	theme	glycoproteins	224:236	arg1	structure					198:206	structure	198:206	structure	198:206	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	5	49	theme	time-of-flight	777:790	arg1	Q-TOF-MS					811:818	Q-TOF-MS	811:818	Q-TOF-MS	811:818	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	49	theme	time-of-flight	777:790	arg1	spectrometry					797:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry	751:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS)	751:819	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	50	theme	different	885:893	arg1	compositions					911:922	29 different oligosaccharide compositions	882:922	29 different oligosaccharide compositions	882:922	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	2	51	from	glycoprotein	453:464	arg1	serum					475:479	human serum	469:479	human serum	469:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	4	52	theme	glycopeptides	690:702	arg1	quantitation					667:678	quantitation	667:678	quantitation	667:678	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	4	52	theme	glycopeptides	690:702	arg1	profiling					653:661	comprehensive profiling	639:661	comprehensive profiling	639:661	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	5	53	theme	mass	792:795	arg1	Q-TOF-MS					811:818	Q-TOF-MS	811:818	Q-TOF-MS	811:818	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	5	53	theme	mass	792:795	arg1	spectrometry					797:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry	751:808	mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS)	751:819	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	4	54	theme	IgG-Fc	683:688	arg1	glycopeptides					690:702	IgG-Fc glycopeptides	683:702	IgG-Fc glycopeptides	683:702	We herein report an integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides.					
31154130	7	55	theme	human	1158:1162	arg1	serum					1164:1168	human serum	1158:1168	human serum	1158:1168	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	7	56	gly	glycopeptides	1195:1207	arg2	glycopeptides					1195:1207	17 sialylated glycopeptides	1181:1207	17 sialylated glycopeptides	1181:1207	As a result, 83 IgG-Fc glycopeptides from human serum, including 17 sialylated glycopeptides, were identified.					
31154130	2	57	dep	IgG1	381:384	arg1	IgG3					393:396	IgG3	393:396	IgG3	393:396	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	2	57	dep	IgG1	381:384	arg1	IgG4					402:405	IgG4	402:405	IgG4	402:405	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	3	58	theme	physiological	556:568	arg1	states					587:592	various physiological and pathological states	548:592	various physiological and pathological states	548:592	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	8	59	theme	UHPLC	1327:1331	arg1	quadrupole					1340:1349	UHPLC triple quadrupole	1327:1349	UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients	1327:1455	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	8	59	theme	UHPLC	1327:1331	arg1	QQQ					1352:1354	QQQ	1352:1354	QQQ	1352:1354	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	9	60	from	IgG4	1580:1583	arg1	species					1567:1573	11 species	1564:1573	11 species from IgG4	1564:1583	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	60	from	IgG4	1580:1583	arg1	species					1540:1546	12 species	1537:1546	12 species from IgG2/3	1537:1558	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	60	from	IgG4	1580:1583	arg1	species					1518:1524	13 species	1515:1524	13 species from IgG1	1515:1534	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	6	61	theme	performance	1053:1063	arg1	UHPLC					1088:1092	UHPLC	1088:1092	UHPLC	1088:1092	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	6	61	theme	performance	1053:1063	arg1	chromatography					1072:1085	ultra high performance liquid chromatography	1042:1085	ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS	1042:1113	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	3	62	theme	IgG-Fc	503:508	arg1	moiety					510:515	IgG-Fc moiety	503:515	IgG-Fc moiety	503:515	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	0	63	with	quantitation	55:66	arg1	application					97:107	application	97:107	application to rheumatoid arthritis	97:131	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	8	64	theme	triple	1333:1338	arg1	quadrupole					1340:1349	UHPLC triple quadrupole	1327:1349	UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients	1327:1455	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	8	64	theme	triple	1333:1338	arg1	QQQ					1352:1354	QQQ	1352:1354	QQQ	1352:1354	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	6	65	theme	high	1048:1051	arg1	UHPLC					1088:1092	UHPLC	1088:1092	UHPLC	1088:1092	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	6	65	theme	high	1048:1051	arg1	chromatography					1072:1085	ultra high performance liquid chromatography	1042:1085	ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS	1042:1113	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	0	66	theme	IgG-Fc	71:76	arg1	glycopeptides					78:90	IgG-Fc glycopeptides	71:90	IgG-Fc glycopeptides	71:90	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	6	67	theme	liquid	1065:1070	arg1	UHPLC					1088:1092	UHPLC	1088:1092	UHPLC	1088:1092	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	6	67	theme	liquid	1065:1070	arg1	chromatography					1072:1085	ultra high performance liquid chromatography	1042:1085	ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS	1042:1113	Secondly, subclass-specific glycopeptides were identified on the basis of high-resolution MS and MS/MS data by using ultra high performance liquid chromatography (UHPLC) coupled to Q-TOF-MS.					
31154130	8	68	theme	RA	1445:1446	arg1	patients					1448:1455	RA patients	1445:1455	RA patients	1445:1455	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	9	69	from	IgG2/3	1553:1558	arg1	species					1567:1573	11 species	1564:1573	11 species from IgG4	1564:1583	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	69	from	IgG2/3	1553:1558	arg1	species					1540:1546	12 species	1537:1546	12 species from IgG2/3	1537:1558	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	9	69	from	IgG2/3	1553:1558	arg1	species					1518:1524	13 species	1515:1524	13 species from IgG1	1515:1534	Finally, 36 potential glycopeptide biomarkers, including 13 species from IgG1, 12 species from IgG2/3 and 11 species from IgG4 were identified.					
31154130	1	70	theme	important	157:165	arg1	role					167:170	an important role	154:170	an important role	154:170	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	8	71	theme	quantitation	1242:1253	arg1	method					1255:1260	a quantitation method	1240:1260	a quantitation method with high sensitivity and repeatability	1240:1300	In addition, a quantitation method with high sensitivity and repeatability was established by using UHPLC triple quadrupole (QQQ) MS. We applied this approach to carry out quantitative analysis of IgG glycosylation in RA patients.					
31154130	1	72	theme	aberrant	245:252	arg1	glycosylation					262:274	aberrant protein glycosylation	245:274	aberrant protein glycosylation	245:274	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31154130	2	73	theme	glycoprotein	453:464	arg1	glycoprotein					453:464	the most dominant and significant glycoprotein	419:464	the most dominant and significant glycoprotein in human serum	419:479	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	2	73	theme	glycoprotein	453:464	arg1	one					412:414	one	412:414	one	412:414	Human immunoglobulin G (IgG), which is composed of four subclasses (IgG1, IgG2, IgG3 and IgG4), is one of the most dominant and significant glycoprotein in human serum.					
31154130	3	74	theme	pathological	574:585	arg1	states					587:592	various physiological and pathological states	548:592	various physiological and pathological states	548:592	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	0	75	with	profiling	41:49	arg1	application					97:107	application	97:107	application to rheumatoid arthritis	97:131	An integrated approach for comprehensive profiling and quantitation of IgG-Fc glycopeptides with application to rheumatoid arthritis.					
31154130	5	76	theme	oligosaccharide	895:909	arg1	compositions					911:922	29 different oligosaccharide compositions	882:922	29 different oligosaccharide compositions	882:922	Firstly, IgG N-glycans were profiled by using mAb-Glyco chip quadrupole time-of-flight mass spectrometry (Q-TOF-MS), resulting characterization of 87 N‑glycans originating from 29 different oligosaccharide compositions.					
31154130	3	77	from	glycosylation	486:498	arg1	moiety					510:515	IgG-Fc moiety	503:515	IgG-Fc moiety	503:515	The glycosylation on IgG-Fc moiety is known to be alternated with various physiological and pathological states.					
31154130	1	78	theme	protein	254:260	arg1	glycosylation					262:274	aberrant protein glycosylation	245:274	aberrant protein glycosylation	245:274	Glycosylation plays an important role in the maintenance of the structure and function of glycoproteins, while aberrant protein glycosylation is correlated with various diseases.					
31216452	0	0	theme	Impact	96:101	arg1	Activities					114:123	Impact Biological Activities	96:123	Impact Biological Activities	96:123	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	8	1	theme	cell	1364:1367	arg1	stimulation					1369:1379	erythroid precursor cell stimulation	1344:1379	erythroid precursor cell stimulation in mice	1344:1387	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	1	2	theme	patients	262:269	arg1	treatment					242:250	treatment	242:250	treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy	242:331	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	6	3	theme	tertiary	1091:1098	arg1	structures					1108:1117	their secondary and tertiary protein structures	1071:1117	their secondary and tertiary protein structures	1071:1117	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	7	4	theme	EPOs	1167:1170	arg1	batches					1172:1178	Eight recombinant EPOs batches	1149:1178	Eight recombinant EPOs batches	1149:1178	Eight recombinant EPOs batches were produced under the same condition.					
31216452	1	5	theme	glycoprotein	173:184	arg1	factor					193:198	a glycoprotein growth factor	171:198	a glycoprotein growth factor	171:198	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	6	6	theme	various	1035:1041	arg1	I-numbers					1043:1051	various I-numbers	1035:1051	various I-numbers	1035:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	7	7	theme	recombinant	1155:1165	arg1	batches					1172:1178	Eight recombinant EPOs batches	1149:1178	Eight recombinant EPOs batches	1149:1178	Eight recombinant EPOs batches were produced under the same condition.					
31216452	8	8	from	stimulation	1369:1379	arg1	mice					1384:1387	mice	1384:1387	mice	1384:1387	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	5	9	gly	sialylation	802:812	arg1	Correlation					738:748	Correlation	738:748	Correlation between bioactivity and glycosylation with terminal sialylation	738:812	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	3	10	theme	negative	583:590	arg1	protein					600:606	more negative charged protein	578:606	more negative charged protein	578:606	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	1	11	theme	chronic	276:282	arg1	failure					290:296	chronic renal failure	276:296	chronic renal failure	276:296	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	0	12	theme	Biological	103:112	arg1	Activities					114:123	Impact Biological Activities	96:123	Impact Biological Activities	96:123	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	6	13	from	effect	1061:1066	arg1	efficacy					1139:1146	measured in vivo efficacy	1122:1146	measured in vivo efficacy	1122:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	9	14	theme	structural	1431:1440	arg1	studies					1442:1448	structural studies	1431:1448	structural studies	1431:1448	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	1	15	theme	renal	284:288	arg1	failure					290:296	chronic renal failure	276:296	chronic renal failure	276:296	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	3	16	theme	glycosylation	501:513	arg1	modification					515:526	glycosylation modification	501:526	glycosylation modification	501:526	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	5	17	theme	profile	886:892	arg1	amount					869:874	the amount	865:874	the amount of charge profile of the EPO variants called "I-number	865:929	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	5	17	theme	profile	886:892	arg1	profile					886:892	charge profile	879:892	charge profile of the EPO variants	879:912	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	3	18	theme	acid	551:554	arg1	antenna					556:562	more sialic acid antenna	539:562	more sialic acid antenna	539:562	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	6	19	theme	structures	1108:1117	arg1	relationship					967:978	the relationship	963:978	the relationship between bioactivities of different EPOs that contained various I-numbers	963:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	6	19	theme	structures	1108:1117	arg1	effect					1061:1066	the effect	1057:1066	the effect of their secondary and tertiary protein structures on measured in vivo efficacy	1057:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	1	20	theme	growth	186:191	arg1	factor					193:198	a glycoprotein growth factor	171:198	a glycoprotein growth factor	171:198	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	6	21	theme	measured	1122:1129	arg1	efficacy					1139:1146	measured in vivo efficacy	1122:1146	measured in vivo efficacy	1122:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	6	22	theme	in vivo	1131:1137	arg1	efficacy					1139:1146	measured in vivo efficacy	1122:1146	measured in vivo efficacy	1122:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	4	23	theme	moieties	680:687	arg1	numbers					660:666	numbers	660:666	numbers of N-glycan moieties	660:687	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	5	24	dep	achieved	831:838	arg1	"					931:931	"	931:931	"	931:931	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	2	25	theme	Biological	334:343	arg1	activity					345:352	Biological activity	334:352	Biological activity	334:352	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	2	25	theme	Biological	334:343	arg1	time					370:373	circulation time	358:373	circulation time	358:373	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	2	25	theme	Biological	334:343	arg1	parameters					381:390	2 parameters	379:390	2 parameters that are important to achieve EPO's efficacy	379:435	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	3	26	theme	Previous	438:445	arg1	efforts					447:453	Previous efforts	438:453	Previous efforts for increasing EPO's efficacy	438:483	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	0	27	theme	Aggregate	0:8	arg1	Forms					10:14	Aggregate Forms	0:14	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile	0:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	6	28	theme	EPOs	1015:1018	arg1	relationship					967:978	the relationship	963:978	the relationship between bioactivities of different EPOs that contained various I-numbers	963:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	6	28	theme	EPOs	1015:1018	arg1	effect					1061:1066	the effect	1057:1066	the effect of their secondary and tertiary protein structures on measured in vivo efficacy	1057:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	5	29	theme	EPO	901:903	arg1	variants					905:912	the EPO variants	897:912	the EPO variants	897:912	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	5	30	theme	variants	905:912	arg1	profile					886:892	charge profile	879:892	charge profile of the EPO variants	879:912	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	6	31	theme	protein	1100:1106	arg1	structures					1108:1117	their secondary and tertiary protein structures	1071:1117	their secondary and tertiary protein structures	1071:1117	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	0	32	theme	Recombinant	19:29	arg1	Erythropoietin					37:50	Recombinant Human Erythropoietin	19:50	Recombinant Human Erythropoietin With Different Charge Profile	19:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	6	33	theme	different	1005:1013	arg1	EPOs					1015:1018	different EPOs	1005:1018	different EPOs that contained various I-numbers	1005:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	6	34	theme	secondary	1077:1085	arg1	structures					1108:1117	their secondary and tertiary protein structures	1071:1117	their secondary and tertiary protein structures	1071:1117	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	5	35	theme	charge	879:884	arg1	profile					886:892	charge profile	879:892	charge profile of the EPO variants	879:912	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	6	36	contain	contained	1025:1033	arg2	I-numbers					1043:1051	various I-numbers	1035:1051	various I-numbers	1035:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	6	36	contain	contained	1025:1033	arg1	EPOs					1015:1018	different EPOs	1005:1018	different EPOs that contained various I-numbers	1005:1051	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	9	37	from	changes	1501:1507	arg1	structure					1520:1528	protein structure	1512:1528	protein structure	1512:1528	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	9	37	from	changes	1501:1507	arg1	presence					1534:1541	presence	1534:1541	presence	1534:1541	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	1	38	theme	Recombinant	126:136	arg1	rHuEPO					160:165	rHuEPO	160:165	rHuEPO	160:165	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	1	38	theme	Recombinant	126:136	arg1	erythropoietin					144:157	Recombinant human erythropoietin	126:157	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor	126:198	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	4	39	theme	acids	713:717	arg1	presence					694:701	presence	694:701	presence of sialic acids at their terminus	694:735	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	0	40	theme	Erythropoietin	37:50	arg1	Forms					10:14	Aggregate Forms	0:14	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile	0:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	8	41	theme	precursor	1354:1362	arg1	cell					1364:1367	erythroid precursor cell	1344:1367	erythroid precursor cell stimulation in mice	1344:1387	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	4	42	theme	N-glycan	671:678	arg1	moieties					680:687	N-glycan moieties	671:687	N-glycan moieties	671:687	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	1	43	theme	biological	222:231	arg1	drug					233:236	a biological drug	220:236	a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy	220:331	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	0	44	theme	Human	31:35	arg1	Erythropoietin					37:50	Recombinant Human Erythropoietin	19:50	Recombinant Human Erythropoietin With Different Charge Profile	19:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	1	45	theme	cancer	313:318	arg1	chemotherapy					320:331	cancer chemotherapy	313:331	cancer chemotherapy	313:331	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	7	46	theme	same	1204:1207	arg1	condition					1209:1217	the same condition	1200:1217	the same condition	1200:1217	Eight recombinant EPOs batches were produced under the same condition.					
31216452	4	47	attach	presence	694:701	arg1	terminus					728:735	their terminus	722:735	their terminus	722:735	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	4	47	attach	presence	694:701	arg2	acids					713:717	sialic acids	706:717	sialic acids	706:717	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	5	48	theme	amount	869:874	arg1	calculation					850:860	the calculation	846:860	the calculation of the amount of charge profile of the EPO variants called "I-number	846:929	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	9	49	theme	species	1557:1563	arg1	structure					1520:1528	protein structure	1512:1528	protein structure	1512:1528	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	9	49	theme	species	1557:1563	arg1	presence					1534:1541	presence	1534:1541	presence	1534:1541	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	0	50	theme	Different	57:65	arg1	Profile					74:80	Different Charge Profile	57:80	Different Charge Profile	57:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	9	51	theme	protein	1512:1518	arg1	structure					1520:1528	protein structure	1512:1528	protein structure	1512:1528	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	8	52	theme	charge	1249:1254	arg1	profiles					1256:1263	charge profiles	1249:1263	EPO's charge profiles determination using capillary electrophoresis and activities	1243:1324	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	9	53	theme	aggregated	1546:1555	arg1	species					1557:1563	aggregated species	1546:1563	aggregated species	1546:1563	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	1	54	theme	human	138:142	arg1	rHuEPO					160:165	rHuEPO	160:165	rHuEPO	160:165	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	1	54	theme	human	138:142	arg1	erythropoietin					144:157	Recombinant human erythropoietin	126:157	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor	126:198	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	8	55	dep	EPO	1243:1245	arg1	profiles					1256:1263	charge profiles	1249:1263	EPO's charge profiles determination using capillary electrophoresis and activities	1243:1324	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	1	56	with	patients	262:269	arg1	failure					290:296	chronic renal failure	276:296	chronic renal failure	276:296	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	2	57	theme	circulation	358:368	arg1	activity					345:352	Biological activity	334:352	Biological activity	334:352	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	2	57	theme	circulation	358:368	arg1	time					370:373	circulation time	358:373	circulation time	358:373	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	2	57	theme	circulation	358:368	arg1	parameters					381:390	2 parameters	379:390	2 parameters that are important to achieve EPO's efficacy	379:435	Biological activity and circulation time are 2 parameters that are important to achieve EPO's efficacy.					
31216452	3	58	theme	charged	592:598	arg1	protein					600:606	more negative charged protein	578:606	more negative charged protein	578:606	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	1	59	gly	glycoprotein	173:184	arg1	glycoprotein					173:184	a glycoprotein growth factor	171:198	a glycoprotein growth factor	171:198	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
31216452	6	60	from	relationship	967:978	arg1	efficacy					1139:1146	measured in vivo efficacy	1122:1146	measured in vivo efficacy	1122:1146	Here, we studied and compared the relationship between bioactivities of different EPOs that contained various I-numbers and the effect of their secondary and tertiary protein structures on measured in vivo efficacy.					
31216452	8	61	theme	erythroid	1344:1352	arg1	cell					1364:1367	erythroid precursor cell	1344:1367	erythroid precursor cell stimulation in mice	1344:1387	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	9	62	theme	conformational	1486:1499	arg1	changes					1501:1507	conformational changes	1486:1507	conformational changes in protein structure and presence of aggregated species	1486:1563	Analyzing the bioactivity, I-number, and structural studies revealed that in spite of I-number, conformational changes in protein structure and presence of aggregated species impact bioactivity substantially.					
31216452	4	63	theme	sialic	706:711	arg1	acids					713:717	sialic acids	706:717	sialic acids	706:717	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	5	64	theme	terminal	793:800	arg1	sialylation					802:812	terminal sialylation	793:812	terminal sialylation	793:812	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	5	65	with	Correlation	738:748	arg1	sialylation					802:812	terminal sialylation	793:812	terminal sialylation	793:812	Correlation between bioactivity and glycosylation with terminal sialylation is theoretically achieved using the calculation of the amount of charge profile of the EPO variants called "I-number."					
31216452	3	66	theme	more	539:542	arg1	antenna					556:562	more sialic acid antenna	539:562	more sialic acid antenna	539:562	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	4	67	from	terminus	728:735	arg1	presence					694:701	presence	694:701	presence of sialic acids at their terminus	694:735	Evidences cleared that EPO's activity increased by numbers of N-glycan moieties with presence of sialic acids at their terminus.					
31216452	0	68	theme	Charge	67:72	arg1	Profile					74:80	Different Charge Profile	57:80	Different Charge Profile	57:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	8	69	theme	capillary	1285:1293	arg1	electrophoresis					1295:1309	capillary electrophoresis	1285:1309	capillary electrophoresis	1285:1309	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	8	70	theme	EPO	1243:1245	arg1	determination					1265:1277	EPO's charge profiles determination	1243:1277	EPO's charge profiles determination using capillary electrophoresis and activities	1243:1324	I-numbers found out by EPO's charge profiles determination using capillary electrophoresis and activities were studied upon erythroid precursor cell stimulation in mice.					
31216452	0	71	with	Erythropoietin	37:50	arg1	Profile					74:80	Different Charge Profile	57:80	Different Charge Profile	57:80	Aggregate Forms of Recombinant Human Erythropoietin With Different Charge Profile Substantially Impact Biological Activities.					
31216452	3	72	theme	sialic	544:549	arg1	acid					551:554	sialic acid	544:554	more sialic acid antenna	539:562	Previous efforts for increasing EPO's efficacy have focused on glycosylation modification via adding more sialic acid antenna and generates more negative charged protein.					
31216452	1	73	theme	anemic	255:260	arg1	patients					262:269	anemic patients	255:269	anemic patients with chronic renal failure or who receive cancer chemotherapy	255:331	Recombinant human erythropoietin (rHuEPO) as a glycoprotein growth factor has been considered a biological drug for treatment of anemic patients with chronic renal failure or who receive cancer chemotherapy.					
29405497	0	0	theme	monocytogenes	78:90	arg1	insights					11:18	Structural insights	0:18	Structural insights into the binding	0:35	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	0	0	theme	monocytogenes	78:90	arg1	mechanisms					51:60	catalytic mechanisms	41:60	catalytic mechanisms of the Listeria monocytogenes	41:90	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	5	1	dep	acids	845:849	arg1	Trp10					862:866	Trp10	862:866	Trp10	862:866	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	1	dep	acids	845:849	arg1	Glu98					852:856	Glu98	852:856	Glu98	852:856	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	1	dep	acids	845:849	arg1	acids					845:849	key amino acids	835:849	key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization	835:917	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	4	2	theme	GH-25	707:711	arg1	family					713:718	the GH-25 family	703:718	the GH-25 family of glycosyl hydrolases	703:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	1	3	theme	cycle	275:279	arg1	end					252:254	the end	248:254	the end of the phage lytic cycle	248:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	0	4	theme	Listeria	69:76	arg1	monocytogenes					78:90	the Listeria monocytogenes	65:90	the Listeria monocytogenes	65:90	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	9	5	theme	structural	1488:1497	arg1	similarity					1499:1508	structural similarity	1488:1508	structural similarity of individual domains	1488:1530	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	8	6	theme	Listeria	1240:1247	arg1	endolysin					1255:1263	a different Listeria phage endolysin	1228:1263	a different Listeria phage endolysin	1228:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	6	7	theme	SH3	1010:1012	arg1	domains					1023:1029	SH3 and LysM domains	1010:1029	domains	1023:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	0	8	theme	glycosyl	106:113	arg1	PlyP40					125:130	bacteriophage glycosyl hydrolase PlyP40	92:130	bacteriophage glycosyl hydrolase PlyP40	92:130	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	0	9	dep	PlyP40	125:130	arg1	insights					11:18	Structural insights	0:18	Structural insights into the binding	0:35	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	0	9	dep	PlyP40	125:130	arg1	mechanisms					51:60	catalytic mechanisms	41:60	catalytic mechanisms of the Listeria monocytogenes	41:90	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	9	10	theme	Listeria	1456:1463	arg1	cells					1465:1469	Listeria cells	1456:1469	Listeria cells	1456:1469	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	0	11	theme	bacteriophage	92:104	arg1	PlyP40					125:130	bacteriophage glycosyl hydrolase PlyP40	92:130	bacteriophage glycosyl hydrolase PlyP40	92:130	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	7	12	theme	cell	1128:1131	arg1	wall					1133:1136	cell wall recognition and lysis	1128:1158	wall	1133:1136	Truncation analysis revealed that both domains are required for full activity but contribute to cell wall recognition and lysis differently.					
29405497	2	13	theme	modular	306:312	arg1	architecture					314:325	a distinct modular architecture	295:325	a distinct modular architecture	295:325	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	1	14	theme	bacteriophage-encoded	148:168	arg1	hydrolases					184:193	bacteriophage-encoded peptidoglycan hydrolases	148:193	bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle	148:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	1	14	theme	bacteriophage-encoded	148:168	arg1	Endolysins					133:142	Endolysins	133:142	Endolysins	133:142	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	9	15	theme	individual	1513:1522	arg1	domains					1524:1530	individual domains	1513:1530	individual domains	1513:1530	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	9	16	with	chimera	1426:1432	arg1	activity					1439:1446	activity	1439:1446	activity against Listeria cells	1439:1469	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	2	17	theme	distinct	297:304	arg1	architecture					314:325	a distinct modular architecture	295:325	a distinct modular architecture	295:325	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	3	18	theme	enzymes	452:458	arg1	analysis					427:434	Structural analysis	416:434	Structural analysis of the complete enzymes or individual domains	416:480	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	0	19	theme	hydrolase	115:123	arg1	PlyP40					125:130	bacteriophage glycosyl hydrolase PlyP40	92:130	bacteriophage glycosyl hydrolase PlyP40	92:130	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	9	20	theme	domain	1332:1337	arg1	swapping					1339:1346	domain swapping	1332:1346	domain swapping between PlyP40 and the streptococcal endolysin Cpl-1	1332:1399	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	4	21	theme	Listeria	782:789	arg1	phages					791:796	Listeria phages	782:796	Listeria phages	782:796	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	5	22	theme	key	835:837	arg1	Trp10					862:866	Trp10	862:866	Trp10	862:866	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	22	theme	key	835:837	arg1	Glu98					852:856	Glu98	852:856	Glu98	852:856	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	22	theme	key	835:837	arg1	acids					845:849	key amino acids	835:849	key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization	835:917	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	3	23	theme	degradation	551:561	arg1	mechanisms					523:532	the mechanisms	519:532	the mechanisms of peptidoglycan degradation	519:561	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	7	24	theme	full	1096:1099	arg1	activity					1101:1108	full activity	1096:1108	full activity	1096:1108	Truncation analysis revealed that both domains are required for full activity but contribute to cell wall recognition and lysis differently.					
29405497	3	25	theme	Structural	416:425	arg1	analysis					427:434	Structural analysis	416:434	Structural analysis of the complete enzymes or individual domains	416:480	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	1	26	theme	bacterial	225:233	arg1	wall					240:243	the bacterial cell wall	221:243	the bacterial cell wall at the end of the phage lytic cycle	221:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	0	27	theme	Structural	0:9	arg1	insights					11:18	Structural insights	0:18	Structural insights into the binding	0:35	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	8	28	theme	CBDP40	1188:1193	arg1	Replacement					1173:1183	Replacement	1173:1183	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin	1173:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	4	29	theme	glycosyl	723:730	arg1	hydrolases					732:741	glycosyl hydrolases	723:741	glycosyl hydrolases	723:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	1	30	theme	cell	235:238	arg1	wall					240:243	the bacterial cell wall	221:243	the bacterial cell wall at the end of the phage lytic cycle	221:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	7	31	dep	wall	1133:1136	arg1	recognition					1138:1148	recognition	1138:1148	recognition	1138:1148	Truncation analysis revealed that both domains are required for full activity but contribute to cell wall recognition and lysis differently.					
29405497	5	32	theme	amino	839:843	arg1	Trp10					862:866	Trp10	862:866	Trp10	862:866	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	32	theme	amino	839:843	arg1	Glu98					852:856	Glu98	852:856	Glu98	852:856	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	5	32	theme	amino	839:843	arg1	acids					845:849	key amino acids	835:849	key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization	835:917	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	8	33	with	Replacement	1173:1183	arg1	domain					1216:1221	a corresponding domain	1200:1221	a corresponding domain from a different Listeria phage endolysin	1200:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	7	34	theme	Truncation	1032:1041	arg1	analysis					1043:1050	Truncation analysis	1032:1050	Truncation analysis	1032:1050	Truncation analysis revealed that both domains are required for full activity but contribute to cell wall recognition and lysis differently.					
29405497	6	35	theme	CBD	981:983	arg1	modules					985:991	two heterogeneous CBD modules	963:991	two heterogeneous CBD modules with homology to SH3 and LysM domains	963:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	3	36	theme	rational	595:602	arg1	design					604:609	the rational design	591:609	the rational design of chimeric enzymes	591:629	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	3	37	theme	peptidoglycan	537:549	arg1	degradation					551:561	peptidoglycan degradation	537:561	peptidoglycan degradation	537:561	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	8	38	theme	different	1230:1238	arg1	endolysin					1255:1263	a different Listeria phage endolysin	1228:1263	a different Listeria phage endolysin	1228:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	6	39	theme	heterogeneous	967:979	arg1	modules					985:991	two heterogeneous CBD modules	963:991	two heterogeneous CBD modules with homology to SH3 and LysM domains	963:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	9	40	theme	enzyme	1543:1548	arg1	function					1550:1557	enzyme function	1543:1557	enzyme function	1543:1557	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	4	41	theme	EAD	676:678	arg1	structure					659:667	the crystal structure	647:667	the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases	647:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	5	42	dep	catalysis	881:889	arg1	stabilization					905:917	stabilization	905:917	stabilization	905:917	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	3	43	theme	complete	443:450	arg1	enzymes					452:458	the complete enzymes	439:458	the complete enzymes	439:458	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	3	44	theme	individual	463:472	arg1	domains					474:480	individual domains	463:480	individual domains	463:480	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	4	45	theme	PlyP40	683:688	arg1	EAD					676:678	the EAD	672:678	the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases	672:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	9	46	theme	streptococcal	1371:1383	arg1	Cpl-1					1395:1399	the streptococcal endolysin Cpl-1	1367:1399	the streptococcal endolysin Cpl-1	1367:1399	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	5	47	theme	Site-directed	799:811	arg1	mutagenesis					813:823	Site-directed mutagenesis	799:823	Site-directed mutagenesis	799:823	Site-directed mutagenesis confirmed key amino acids (Glu98 and Trp10) involved in catalysis and substrate stabilization.					
29405497	4	48	theme	crystal	651:657	arg1	structure					659:667	the crystal structure	647:667	the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases	647:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	3	49	theme	domains	474:480	arg1	analysis					427:434	Structural analysis	416:434	Structural analysis of the complete enzymes or individual domains	416:480	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	8	50	theme	phage	1249:1253	arg1	endolysin					1255:1263	a different Listeria phage endolysin	1228:1263	a different Listeria phage endolysin	1228:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	9	51	theme	endolysin	1385:1393	arg1	Cpl-1					1395:1399	the streptococcal endolysin Cpl-1	1367:1399	the streptococcal endolysin Cpl-1	1367:1399	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	3	52	theme	chimeric	614:621	arg1	enzymes					623:629	chimeric enzymes	614:629	chimeric enzymes	614:629	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	1	53	from	end	252:254	arg1	wall					240:243	the bacterial cell wall	221:243	the bacterial cell wall at the end of the phage lytic cycle	221:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	8	54	theme	pH	1311:1312	arg1	optimum					1314:1320	pH optimum	1311:1320	pH optimum	1311:1320	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	0	55	theme	catalytic	41:49	arg1	mechanisms					51:60	catalytic mechanisms	41:60	catalytic mechanisms of the Listeria monocytogenes	41:90	Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.					
29405497	3	56	theme	enzymes	623:629	arg1	design					604:609	the rational design	591:609	the rational design of chimeric enzymes	591:629	Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes.					
29405497	4	57	theme	family	713:718	arg1	PlyP40					683:688	PlyP40	683:688	PlyP40	683:688	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	4	57	theme	family	713:718	arg1	member					693:698	a member	691:698	a member of the GH-25 family of glycosyl hydrolases	691:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	8	58	theme	corresponding	1202:1214	arg1	domain					1216:1221	a corresponding domain	1200:1221	a corresponding domain from a different Listeria phage endolysin	1200:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	1	59	theme	peptidoglycan	170:182	arg1	hydrolases					184:193	bacteriophage-encoded peptidoglycan hydrolases	148:193	bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle	148:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	1	59	theme	peptidoglycan	170:182	arg1	Endolysins					133:142	Endolysins	133:142	Endolysins	133:142	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	8	60	from	endolysin	1255:1263	arg1	domain					1216:1221	a corresponding domain	1200:1221	a corresponding domain from a different Listeria phage endolysin	1200:1263	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	4	61	theme	hydrolases	732:741	arg1	family					713:718	the GH-25 family	703:718	the GH-25 family of glycosyl hydrolases	703:741	We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages.					
29405497	6	62	with	modules	985:991	arg1	homology					998:1005	homology	998:1005	homology to SH3 and LysM domains	998:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	8	63	from	shift	1302:1306	arg1	optimum					1314:1320	pH optimum	1311:1320	pH optimum	1311:1320	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	2	64	theme	active	356:361	arg1	EADs					372:375	EADs	372:375	EADs	372:375	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	2	64	theme	active	356:361	arg1	domains					363:369	enzymatically active domains	342:369	enzymatically active domains (EADs)	342:376	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	9	65	theme	intergeneric	1413:1424	arg1	chimera					1426:1432	an intergeneric chimera	1410:1432	an intergeneric chimera with activity against Listeria cells	1410:1469	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	9	66	theme	domains	1524:1530	arg1	similarity					1499:1508	structural similarity	1488:1508	structural similarity of individual domains	1488:1530	Finally, domain swapping between PlyP40 and the streptococcal endolysin Cpl-1 produced an intergeneric chimera with activity against Listeria cells, indicating that structural similarity of individual domains determines enzyme function.					
29405497	8	67	theme	significant	1290:1300	arg1	shift					1302:1306	a significant shift	1288:1306	a significant shift in pH optimum	1288:1320	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	6	68	contain	contains	954:961	arg2	modules					985:991	two heterogeneous CBD modules	963:991	two heterogeneous CBD modules with homology to SH3 and LysM domains	963:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	6	68	contain	contains	954:961	arg1	PlyP40					947:952	PlyP40	947:952	PlyP40	947:952	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	2	69	theme	wall-binding	387:398	arg1	CBDs					409:412	CBDs	409:412	CBDs	409:412	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	2	69	theme	wall-binding	387:398	arg1	domains					400:406	cell wall-binding domains	382:406	cell wall-binding domains (CBDs)	382:413	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	1	70	theme	phage	263:267	arg1	cycle					275:279	the phage lytic cycle	259:279	the phage lytic cycle	259:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29405497	8	71	with	enzyme	1276:1281	arg1	shift					1302:1306	a significant shift	1288:1306	a significant shift in pH optimum	1288:1320	Replacement of CBDP40 with a corresponding domain from a different Listeria phage endolysin yielded an enzyme with a significant shift in pH optimum.					
29405497	6	72	theme	LysM	1018:1021	arg1	domains					1023:1029	SH3 and LysM domains	1010:1029	domains	1023:1029	In addition, we found that PlyP40 contains two heterogeneous CBD modules with homology to SH3 and LysM domains.					
29405497	2	73	theme	cell	382:385	arg1	CBDs					409:412	CBDs	409:412	CBDs	409:412	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	2	73	theme	cell	382:385	arg1	domains					400:406	cell wall-binding domains	382:406	cell wall-binding domains (CBDs)	382:413	They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall-binding domains (CBDs).					
29405497	1	74	theme	lytic	269:273	arg1	cycle					275:279	the phage lytic cycle	259:279	the phage lytic cycle	259:279	Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle.					
29546786	7	0	theme	bacteria	1075:1082	arg1	Ags					1068:1070	dominant Ags	1059:1070	dominant Ags of bacteria	1059:1082	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	0	theme	bacteria	1075:1082	arg1	priming					967:973	the priming	963:973	the priming	963:973	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	8	1	theme	contact	1330:1336	arg1	importance					1310:1319	the importance	1306:1319	the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire	1306:1435	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	5	2	theme	anti-glycan	582:592	arg1	Abs					594:596	anti-glycan Abs	582:596	anti-glycan Abs	582:596	Sterile mice did not possess anti-glycan Abs.					
29546786	7	3	dep	glycoproteins	1150:1162	arg1	Abs					1186:1188	Abs	1186:1188	Abs of the ABH blood group system	1186:1218	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	5	4	contain	possess	574:580	arg1	mice					561:564	Sterile mice	553:564	Sterile mice	553:564	Sterile mice did not possess anti-glycan Abs.					
29546786	5	4	contain	possess	574:580	arg2	Abs					594:596	anti-glycan Abs	582:596	anti-glycan Abs	582:596	Sterile mice did not possess anti-glycan Abs.					
29546786	8	5	theme	naïve	1345:1349	arg1	system					1358:1363	a naïve immune system	1343:1363	a naïve immune system	1343:1363	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	6	6	theme	non-sterile	746:756	arg1	food					758:761	non-sterile food	746:761	non-sterile food	746:761	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	6	7	theme	Abs	817:819	arg1	formation					804:812	the formation	800:812	the formation of Abs	800:819	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	8	gly	glycoproteins	1150:1162	arg1	glycoproteins					1150:1162	mammalian glycoproteins	1140:1162	mammalian glycoproteins	1140:1162	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	9	theme	short-term	720:729	arg1	nutrition					731:739	short-term nutrition	720:739	short-term nutrition	720:739	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	10	dep	Abs	1186:1188	arg1	e.g.					1181:1184	e.g.	1181:1184	e.g.	1181:1184	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	11	theme	dominant	1059:1066	arg1	Ags					1068:1070	dominant Ags	1059:1070	dominant Ags of bacteria	1059:1082	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	11	theme	dominant	1059:1066	arg1	priming					967:973	the priming	963:973	the priming	963:973	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	12	theme	typical	1121:1127	arg1	glycans					1129:1135	typical glycans	1121:1135	typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides	1121:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	3	13	theme	anti-glycan	308:318	arg1	Abs					320:322	anti-glycan Abs	308:322	anti-glycan Abs	308:322	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	4	14	theme	mice	421:424	arg1	Abs					414:416	Abs	414:416	Abs of mice primed in this way	414:443	The repertoires of Abs of mice primed in this way were compared using a microarray that included about 350 glycans, as well as 150 bacterial polysaccharides.					
29546786	5	15	theme	Sterile	553:559	arg1	mice					561:564	Sterile mice	553:564	Sterile mice	553:564	Sterile mice did not possess anti-glycan Abs.					
29546786	1	16	theme	Innate	58:63	arg1	Abs					82:84	Innate immunity natural Abs	58:84	Innate immunity natural Abs (NAbs)	58:91	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	1	16	theme	Innate	58:63	arg1	NAbs					87:90	NAbs	87:90	NAbs	87:90	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	3	17	theme	Abs	320:322	arg1	repertoires					293:303	repertoires	293:303	repertoires of anti-glycan Abs	293:322	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	3	17	theme	Abs	320:322	arg1	Abs					320:322	anti-glycan Abs	308:322	anti-glycan Abs	308:322	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	1	18	theme	immunity	65:72	arg1	Abs					82:84	Innate immunity natural Abs	58:84	Innate immunity natural Abs (NAbs)	58:91	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	1	18	theme	immunity	65:72	arg1	NAbs					87:90	NAbs	87:90	NAbs	87:90	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	7	19	from	priming	967:973	arg1	composition					1005:1015	a composition	1003:1015	a composition of the bacterial cell envelope	1003:1046	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	20	from	animals	709:715	arg1	nutrition					731:739	short-term nutrition	720:739	short-term nutrition	720:739	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	8	21	theme	environment	1392:1402	arg1	microorganisms					1370:1383	microorganisms	1370:1383	microorganisms of the environment	1370:1402	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	7	22	contain	have	1233:1236	arg1	glycolipids					1168:1178	glycolipids	1168:1178	glycolipids	1168:1178	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	22	contain	have	1233:1236	arg1	glycoproteins					1150:1162	mammalian glycoproteins	1140:1162	mammalian glycoproteins	1140:1162	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	22	contain	have	1233:1236	arg2	similarity					1251:1260	a structural similarity	1238:1260	a structural similarity to the polysaccharides	1238:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	1	23	theme	natural	74:80	arg1	Abs					82:84	Innate immunity natural Abs	58:84	Innate immunity natural Abs (NAbs)	58:91	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	1	23	theme	natural	74:80	arg1	NAbs					87:90	NAbs	87:90	NAbs	87:90	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	6	24	theme	strain	628:633	arg1	inoculation					604:614	Oral inoculation	599:614	Oral inoculation of a single strain	599:633	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	6	24	theme	strain	628:633	arg1	combination					638:648	combination	638:648	combination of two to four strains of bacteria	638:683	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	3	25	theme	various	377:383	arg1	bacteria					385:392	os various bacteria	374:392	os various bacteria	374:392	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	3	26	theme	mice	351:354	arg1	blood					342:346	the peripheral blood	327:346	the peripheral blood of mice that received per os various bacteria	327:392	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	6	27	theme	mice	878:881	arg1	gavage					839:844	a single gavage	830:844	a single gavage of digested food of non-sterile mice	830:881	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	28	theme	polysaccharide	980:993	arg1	Ags					995:997	polysaccharide Ags	980:997	polysaccharide Ags	980:997	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	29	theme	structural	1240:1249	arg1	similarity					1251:1260	a structural similarity	1238:1260	a structural similarity to the polysaccharides	1238:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	30	theme	single	621:626	arg1	strain					628:633	a single strain	619:633	a single strain	619:633	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	8	31	theme	early	1324:1328	arg1	contact					1330:1336	early contact	1324:1336	early contact with a naïve immune system with microorganisms of the environment	1324:1402	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	3	32	theme	peripheral	331:340	arg1	blood					342:346	the peripheral blood	327:346	the peripheral blood of mice that received per os various bacteria	327:392	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	6	33	theme	non-sterile	866:876	arg1	mice					878:881	non-sterile mice	866:881	non-sterile mice	866:881	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	0	34	theme	Abs	14:16	arg1	Abs					14:16	Abs	14:16	Abs	14:16	Repertoire of Abs primed by bacteria in gnotobiotic mice.					
29546786	0	34	theme	Abs	14:16	arg1	Repertoire					0:9	Repertoire	0:9	Repertoire of Abs	0:16	Repertoire of Abs primed by bacteria in gnotobiotic mice.					
29546786	8	35	theme	immune	1351:1356	arg1	system					1358:1363	a naïve immune system	1343:1363	a naïve immune system	1343:1363	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	7	36	theme	glycolipids	1168:1178	arg1	glycans					1129:1135	typical glycans	1121:1135	typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides	1121:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	37	theme	cell	1034:1037	arg1	envelope					1039:1046	the bacterial cell envelope	1020:1046	the bacterial cell envelope	1020:1046	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	38	theme	food	858:861	arg1	gavage					839:844	a single gavage	830:844	a single gavage of digested food of non-sterile mice	830:881	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	39	theme	mammalian	1140:1148	arg1	glycoproteins					1150:1162	mammalian glycoproteins	1140:1162	mammalian glycoproteins	1140:1162	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	8	40	with	contact	1330:1336	arg1	system					1358:1363	a naïve immune system	1343:1363	a naïve immune system	1343:1363	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	8	40	with	contact	1330:1336	arg1	microorganisms					1370:1383	microorganisms	1370:1383	microorganisms of the environment	1370:1402	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	6	41	theme	natural	934:940	arg1	ones					942:945	the natural ones	930:945	the natural ones	930:945	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	3	42	theme	os	374:375	arg1	bacteria					385:392	os various bacteria	374:392	os various bacteria	374:392	Here, we compared repertoires of anti-glycan Abs in the peripheral blood of mice that received per os various bacteria.					
29546786	6	43	theme	digested	849:856	arg1	food					858:861	digested food	849:861	digested food	849:861	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	44	theme	envelope	1039:1046	arg1	composition					1005:1015	a composition	1003:1015	a composition of the bacterial cell envelope	1003:1046	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	45	theme	glycoproteins	1150:1162	arg1	glycans					1129:1135	typical glycans	1121:1135	typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides	1121:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	0	46	theme	gnotobiotic	40:50	arg1	mice					52:55	gnotobiotic mice	40:55	gnotobiotic mice	40:55	Repertoire of Abs primed by bacteria in gnotobiotic mice.					
29546786	6	47	with	animals	709:715	arg1	food					758:761	non-sterile food	746:761	non-sterile food	746:761	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	4	48	theme	Abs	414:416	arg1	Abs					414:416	Abs	414:416	Abs of mice primed in this way	414:443	The repertoires of Abs of mice primed in this way were compared using a microarray that included about 350 glycans, as well as 150 bacterial polysaccharides.					
29546786	4	48	theme	Abs	414:416	arg1	repertoires					399:409	The repertoires	395:409	The repertoires of Abs of mice primed in this way	395:443	The repertoires of Abs of mice primed in this way were compared using a microarray that included about 350 glycans, as well as 150 bacterial polysaccharides.					
29546786	7	49	theme	system	1213:1218	arg1	Abs					1186:1188	Abs	1186:1188	Abs of the ABH blood group system	1186:1218	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	7	50	theme	bacterial	1024:1032	arg1	envelope					1039:1046	the bacterial cell envelope	1020:1046	the bacterial cell envelope	1020:1046	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	8	51	theme	NAbs	1421:1424	arg1	repertoire					1426:1435	a normal NAbs repertoire	1412:1435	a normal NAbs repertoire	1412:1435	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	6	52	theme	bacteria	676:683	arg1	strains					665:671	two to four strains	653:671	two to four strains of bacteria	653:683	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	8	53	theme	normal	1414:1419	arg1	repertoire					1426:1435	a normal NAbs repertoire	1412:1435	a normal NAbs repertoire	1412:1435	The results support the importance of early contact with a naïve immune system with microorganisms of the environment to form a normal NAbs repertoire.					
29546786	6	54	theme	Oral	599:602	arg1	inoculation					604:614	Oral inoculation	599:614	Oral inoculation of a single strain	599:633	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	6	55	theme	single	832:837	arg1	gavage					839:844	a single gavage	830:844	a single gavage of digested food of non-sterile mice	830:881	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	56	theme	blood	1201:1205	arg1	system					1213:1218	the ABH blood group system	1193:1218	the ABH blood group system	1193:1218	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	2	57	theme	NAbs	229:232	arg1	formation					216:224	the formation	212:224	the formation of NAbs	212:232	Despite active research, the stimuli that induce the formation of NAbs are still described only hypothetically.					
29546786	2	58	theme	active	171:176	arg1	research					178:185	active research	171:185	active research	171:185	Despite active research, the stimuli that induce the formation of NAbs are still described only hypothetically.					
29546786	7	59	theme	group	1207:1211	arg1	system					1213:1218	the ABH blood group system	1193:1218	the ABH blood group system	1193:1218	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	60	theme	strains	665:671	arg1	inoculation					604:614	Oral inoculation	599:614	Oral inoculation of a single strain	599:633	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	6	60	theme	strains	665:671	arg1	combination					638:648	combination	638:648	combination of two to four strains of bacteria	638:683	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	61	theme	Abs	1109:1111	arg1	induction					1096:1104	the induction	1092:1104	the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides	1092:1283	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	4	62	theme	bacterial	526:534	arg1	polysaccharides					536:550	150 bacterial polysaccharides	522:550	150 bacterial polysaccharides	522:550	The repertoires of Abs of mice primed in this way were compared using a microarray that included about 350 glycans, as well as 150 bacterial polysaccharides.					
29546786	4	62	theme	bacterial	526:534	arg1	glycans					502:508	about 350 glycans	492:508	about 350 glycans	492:508	The repertoires of Abs of mice primed in this way were compared using a microarray that included about 350 glycans, as well as 150 bacterial polysaccharides.					
29546786	7	63	with	priming	967:973	arg1	Ags					995:997	polysaccharide Ags	980:997	polysaccharide Ags	980:997	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	6	64	dep	four	660:663	arg1	to					657:658	to	657:658	to	657:658	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	6	65	theme	repertoire	910:919	arg1	formation					895:903	the formation	891:903	the formation of a repertoire close to the natural ones	891:945	Oral inoculation of a single strain or combination of two to four strains of bacteria, as well as putting the animals on short-term nutrition with non-sterile food, did not contribute significantly to the formation of Abs, whereas a single gavage of digested food of non-sterile mice induced the formation of a repertoire close to the natural ones.					
29546786	7	66	theme	ABH	1197:1199	arg1	system					1213:1218	the ABH blood group system	1193:1218	the ABH blood group system	1193:1218	Interestingly, the priming with polysaccharide Ags (in a composition of the bacterial cell envelope), that is, dominant Ags of bacteria, led to the induction of Abs against typical glycans of mammalian glycoproteins and glycolipids (e.g. Abs of the ABH blood group system) that do not have a structural similarity to the polysaccharides.					
29546786	1	67	theme	functions	113:121	arg1	surveillance					149:160	surveillance	149:160	surveillance	149:160	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	1	67	theme	functions	113:121	arg1	protection					134:143	protection	134:143	protection	134:143	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
29546786	1	67	theme	functions	113:121	arg1	number					103:108	a number	101:108	a number of functions, including protection and surveillance	101:160	Innate immunity natural Abs (NAbs) execute a number of functions, including protection and surveillance.					
31751705	1	0	with	first	310:314	arg1	M3FX					350:353	M3FX	350:353	M3FX	350:353	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	0	with	first	310:314	arg1	N-glycan					340:347	plant complex type N-glycan	321:347	plant complex type N-glycan (M3FX)	321:354	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	1	theme	receptors	174:182	arg1	discovery					130:138	the discovery	126:138	the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants	126:217	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	7	2	theme	rapid	1558:1562	arg1	purification					1564:1575	rapid purification	1558:1575	rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract	1558:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	5	3	theme	amino	1159:1163	arg1	analysis					1182:1189	amino acid composition analysis	1159:1189	amino acid composition analysis after acid hydrolysis	1159:1211	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	5	4	theme	N-glycans	1107:1115	arg1	incorporation					1090:1102	The incorporation	1086:1102	The incorporation of N-glycans into γ-L-PGA (mol%)	1086:1135	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	7	5	theme	mannose-binding	1580:1594	arg1	protein					1596:1602	mannose-binding protein	1580:1602	mannose-binding protein	1580:1602	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	5	theme	mannose-binding	1580:1594	arg1	A					1618:1618	Concanavalin A	1605:1618	Concanavalin A	1605:1618	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	1	6	theme	N-glycopeptides	274:288	arg1	types					265:269	three types	259:269	three types of N-glycopeptides	259:288	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	5	7	theme	acid	1165:1168	arg1	analysis					1182:1189	amino acid composition analysis	1159:1189	amino acid composition analysis after acid hydrolysis	1159:1211	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	2	8	theme	storage	570:576	arg1	glycoproteins					578:590	storage glycoproteins	570:590	storage glycoproteins of Ginkgo biloba seeds	570:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	9	theme	angularis	622:630	arg1	seeds					632:636	Vigna angularis seeds	616:636	Vigna angularis seeds	616:636	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	0	10	gly	N-glycopeptide	78:91	arg2	N-glycopeptide					78:91	multivalent N-glycopeptide units	66:97	multivalent N-glycopeptide units	66:97	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	2	11	theme	each	691:694	arg1	glycoproteins					696:708	each glycoproteins	691:708	each glycoproteins	691:708	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	5	12	theme	mol	1131:1133	arg1	γ-L-PGA					1122:1128	γ-L-PGA	1122:1128	γ-L-PGA (mol%)	1122:1135	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	5	12	theme	mol	1131:1133	arg1	%					1134:1134	mol%	1131:1134	mol%	1131:1134	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	6	13	theme	Asn-M3FX	1241:1248	arg1	rates					1232:1236	The incorporation rates	1214:1236	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA	1214:1294	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	6	13	theme	Asn-M3FX	1241:1248	arg1	%					1305:1305	15.4%	1301:1305	15.4%	1301:1305	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	1	14	theme	animal	425:430	arg1	N-glycan					445:452	animal complex type N-glycan	425:452	animal complex type N-glycan (NeuAc2Gal2GN2M3)	425:470	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	14	theme	animal	425:430	arg1	NeuAc2Gal2GN2M3					455:469	NeuAc2Gal2GN2M3	455:469	NeuAc2Gal2GN2M3	455:469	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	7	15	contain	carrying	1484:1491	arg1	tool					1549:1552	a useful tool	1540:1552	a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract	1540:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	15	contain	carrying	1484:1491	arg1	neoglycopolymer					1468:1482	the neoglycopolymer	1464:1482	the neoglycopolymer carrying the multivalent high-mannose type N-glycans	1464:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	15	contain	carrying	1484:1491	arg2	N-glycans					1527:1535	the multivalent high-mannose type N-glycans	1493:1535	the multivalent high-mannose type N-glycans	1493:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	3	16	theme	4,6-Dimethoxy-1,3,5-triazin-2-yl	893:924	arg1	chloride					948:955	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate	890:965	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM)	890:974	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	16	theme	4,6-Dimethoxy-1,3,5-triazin-2-yl	893:924	arg1	DMT-MM					968:973	DMT-MM	968:973	DMT-MM	968:973	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	2	17	theme	Asn-oligosaccharide	488:506	arg1	types					479:483	Three types	473:483	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3)	473:549	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	7	18	theme	Concanavalin	1605:1616	arg1	protein					1596:1602	mannose-binding protein	1580:1602	mannose-binding protein	1580:1602	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	18	theme	Concanavalin	1605:1616	arg1	A					1618:1618	Concanavalin A	1605:1618	Concanavalin A	1605:1618	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	1	19	theme	complex	432:438	arg1	N-glycan					445:452	animal complex type N-glycan	425:452	animal complex type N-glycan (NeuAc2Gal2GN2M3)	425:470	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	19	theme	complex	432:438	arg1	NeuAc2Gal2GN2M3					455:469	NeuAc2Gal2GN2M3	455:469	NeuAc2Gal2GN2M3	455:469	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	20	theme	lectin-like	187:197	arg1	receptors					199:207	lectin-like receptors	187:207	lectin-like receptors	187:207	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	2	21	theme	seeds	609:613	arg1	glycoproteins					578:590	storage glycoproteins	570:590	storage glycoproteins of Ginkgo biloba seeds	570:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	21	theme	seeds	609:613	arg1	seeds					632:636	Vigna angularis seeds	616:636	Vigna angularis seeds	616:636	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	21	theme	seeds	609:613	arg1	glycopeptides					652:664	egg yolk glycopeptides	643:664	egg yolk glycopeptides	643:664	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	1	22	gly	N-glycopeptides	274:288	arg2	N-glycopeptides					274:288	N-glycopeptides	274:288	N-glycopeptides	274:288	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	23	theme	receptors	199:207	arg1	discovery					130:138	the discovery	126:138	the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants	126:217	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	24	theme	high-mannose	373:384	arg1	M8					401:402	M8	401:402	M8	401:402	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	24	theme	high-mannose	373:384	arg1	N-glycan					391:398	high-mannose type N-glycan	373:398	high-mannose type N-glycan (M8)	373:403	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	2	25	theme	biloba	602:607	arg1	seeds					609:613	Ginkgo biloba seeds	595:613	Ginkgo biloba seeds	595:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	26	theme	yolk	647:650	arg1	glycopeptides					652:664	egg yolk glycopeptides	643:664	egg yolk glycopeptides	643:664	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	1	27	from	discovery	130:138	arg1	plants					212:217	plants	212:217	plants	212:217	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	7	28	theme	high-mannose	1509:1520	arg1	N-glycans					1527:1535	the multivalent high-mannose type N-glycans	1493:1535	the multivalent high-mannose type N-glycans	1493:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	4	29	theme	gel-filtration	1047:1060	arg1	combination					1032:1042	a combination	1030:1042	a combination of gel-filtration and reverse-phase HPLC	1030:1083	The resulting neoglycopolymers were purified through a combination of gel-filtration and reverse-phase HPLC.					
31751705	2	30	theme	Ginkgo	595:600	arg1	seeds					609:613	Ginkgo biloba seeds	595:613	Ginkgo biloba seeds	595:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	31	theme	egg	643:645	arg1	glycopeptides					652:664	egg yolk glycopeptides	643:664	egg yolk glycopeptides	643:664	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	1	32	theme	present	107:113	arg1	study					115:119	the present study	103:119	the present study	103:119	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	33	theme	n-hydrate	957:965	arg1	chloride					948:955	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate	890:965	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM)	890:974	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	33	theme	n-hydrate	957:965	arg1	DMT-MM					968:973	DMT-MM	968:973	DMT-MM	968:973	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	4	34	theme	HPLC	1080:1083	arg1	combination					1032:1042	a combination	1030:1042	a combination of gel-filtration and reverse-phase HPLC	1030:1083	The resulting neoglycopolymers were purified through a combination of gel-filtration and reverse-phase HPLC.					
31751705	2	35	gly	glycopeptide	713:724	arg2	glycopeptide					713:724	glycopeptide	713:724	glycopeptide	713:724	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	36	dep	Asn-oligosaccharide	488:506	arg1	Asn-M8					519:524	Asn-M8	519:524	Asn-M8	519:524	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	36	dep	Asn-oligosaccharide	488:506	arg1	Asn-NeuAc2Gal2GN2M3					530:548	Asn-NeuAc2Gal2GN2M3	530:548	Asn-NeuAc2Gal2GN2M3	530:548	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	36	dep	Asn-oligosaccharide	488:506	arg1	Asn-M3FX					509:516	Asn-M3FX	509:516	Asn-M3FX	509:516	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	37	theme	Vigna	616:620	arg1	seeds					632:636	Vigna angularis seeds	616:636	Vigna angularis seeds	616:636	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	5	38	theme	composition	1170:1180	arg1	analysis					1182:1189	amino acid composition analysis	1159:1189	amino acid composition analysis after acid hydrolysis	1159:1211	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	7	39	theme	protein	1596:1602	arg1	purification					1564:1575	rapid purification	1558:1575	rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract	1558:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	2	40	gly	glycoproteins	578:590	arg1	glycoproteins					578:590	storage glycoproteins	570:590	storage glycoproteins of Ginkgo biloba seeds	570:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	0	41	theme	preliminary	14:24	arg1	evaluation					26:35	preliminary evaluation	14:35	preliminary evaluation	14:35	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	6	42	theme	incorporation	1218:1230	arg1	rates					1232:1236	The incorporation rates	1214:1236	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA	1214:1294	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	6	42	theme	incorporation	1218:1230	arg1	%					1305:1305	15.4%	1301:1305	15.4%	1301:1305	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	1	43	theme	type	386:389	arg1	M8					401:402	M8	401:402	M8	401:402	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	43	theme	type	386:389	arg1	N-glycan					391:398	high-mannose type N-glycan	373:398	high-mannose type N-glycan (M8)	373:403	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	44	theme	-4-methylmorpholinium	926:946	arg1	chloride					948:955	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate	890:965	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM)	890:974	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	44	theme	-4-methylmorpholinium	926:946	arg1	DMT-MM					968:973	DMT-MM	968:973	DMT-MM	968:973	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	0	45	theme	neoglycopolymers	40:55	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	0	45	theme	neoglycopolymers	40:55	arg1	evaluation					26:35	preliminary evaluation	14:35	preliminary evaluation	14:35	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	2	46	gly	glycoproteins	696:708	arg1	glycoproteins					696:708	each glycoproteins	691:708	each glycoproteins	691:708	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	7	47	theme	useful	1542:1547	arg1	tool					1549:1552	a useful tool	1540:1552	a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract	1540:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	47	theme	useful	1542:1547	arg1	neoglycopolymer					1468:1482	the neoglycopolymer	1464:1482	the neoglycopolymer carrying the multivalent high-mannose type N-glycans	1464:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	6	48	theme	Asn-NeuAc2Gal2GN2M3	1263:1281	arg1	rates					1232:1236	The incorporation rates	1214:1236	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA	1214:1294	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	6	48	theme	Asn-NeuAc2Gal2GN2M3	1263:1281	arg1	%					1305:1305	15.4%	1301:1305	15.4%	1301:1305	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	7	49	theme	type	1522:1525	arg1	N-glycans					1527:1535	the multivalent high-mannose type N-glycans	1493:1535	the multivalent high-mannose type N-glycans	1493:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	1	50	theme	plant	321:325	arg1	M3FX					350:353	M3FX	350:353	M3FX	350:353	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	50	theme	plant	321:325	arg1	N-glycan					340:347	plant complex type N-glycan	321:347	plant complex type N-glycan (M3FX)	321:354	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	51	theme	carboxyl	833:840	arg1	γ-L-PGA					876:882	γ-L-PGA	876:882	γ-L-PGA	876:882	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	51	theme	carboxyl	833:840	arg1	groups					842:847	the carboxyl groups	829:847	the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA)	829:883	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	51	theme	carboxyl	833:840	arg1	acid					870:873	poly-γ-L-glutamic acid	852:873	poly-γ-L-glutamic acid (γ-L-PGA)	852:883	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	0	52	theme	multivalent	66:76	arg1	units					93:97	multivalent N-glycopeptide units	66:97	multivalent N-glycopeptide units	66:97	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	1	53	theme	complex	327:333	arg1	M3FX					350:353	M3FX	350:353	M3FX	350:353	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	53	theme	complex	327:333	arg1	N-glycan					340:347	plant complex type N-glycan	321:347	plant complex type N-glycan (M3FX)	321:354	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	4	54	theme	resulting	981:989	arg1	neoglycopolymers					991:1006	The resulting neoglycopolymers	977:1006	The resulting neoglycopolymers	977:1006	The resulting neoglycopolymers were purified through a combination of gel-filtration and reverse-phase HPLC.					
31751705	7	55	from	extract	1636:1642	arg1	purification					1564:1575	rapid purification	1558:1575	rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract	1558:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	6	56	theme	N-glycans	1379:1387	arg1	molecules					1366:1374	nearly 890, 500, and 640 molecules	1341:1374	nearly 890, 500, and 640 molecules of N-glycans	1341:1387	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	1	57	theme	type	335:338	arg1	M3FX					350:353	M3FX	350:353	M3FX	350:353	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	57	theme	type	335:338	arg1	N-glycan					340:347	plant complex type N-glycan	321:347	plant complex type N-glycan (M3FX)	321:354	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	58	dep	coupled	818:824	arg1	such					761:764	such	761:764	such	761:764	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	6	59	theme	Asn-M8	1251:1256	arg1	rates					1232:1236	The incorporation rates	1214:1236	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA	1214:1294	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	6	59	theme	Asn-M8	1251:1256	arg1	%					1305:1305	15.4%	1301:1305	15.4%	1301:1305	The incorporation rates of Asn-M3FX, Asn-M8, and Asn-NeuAc2Gal2GN2M3 into γ-L-PGA were 15.4%, 8.6%, and 11.1%, indicating that nearly 890, 500, and 640 molecules of N-glycans were conjugated with γ-L-PGA, respectively.					
31751705	2	60	theme	actinase	671:678	arg1	digests					680:686	actinase digests	671:686	actinase digests of each glycoproteins or glycopeptide	671:724	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	3	61	theme	poly-γ-L-glutamic	852:868	arg1	γ-L-PGA					876:882	γ-L-PGA	876:882	γ-L-PGA	876:882	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	61	theme	poly-γ-L-glutamic	852:868	arg1	acid					870:873	poly-γ-L-glutamic acid	852:873	poly-γ-L-glutamic acid (γ-L-PGA)	852:883	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	5	62	theme	acid	1197:1200	arg1	hydrolysis					1202:1211	acid hydrolysis	1197:1211	acid hydrolysis	1197:1211	The incorporation of N-glycans into γ-L-PGA (mol%) was estimated through amino acid composition analysis after acid hydrolysis.					
31751705	1	63	theme	type	440:443	arg1	N-glycan					445:452	animal complex type N-glycan	425:452	animal complex type N-glycan (NeuAc2Gal2GN2M3)	425:470	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	63	theme	type	440:443	arg1	NeuAc2Gal2GN2M3					455:469	NeuAc2Gal2GN2M3	455:469	NeuAc2Gal2GN2M3	455:469	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	64	theme	acid	870:873	arg1	γ-L-PGA					876:882	γ-L-PGA	876:882	γ-L-PGA	876:882	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	64	theme	acid	870:873	arg1	groups					842:847	the carboxyl groups	829:847	the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA)	829:883	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	64	theme	acid	870:873	arg1	acid					870:873	poly-γ-L-glutamic acid	852:873	poly-γ-L-glutamic acid (γ-L-PGA)	852:883	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	65	theme	Asn-oligosaccharide	793:811	arg1	Asn-oligosaccharide					793:811	Asn-oligosaccharide	793:811	Asn-oligosaccharide	793:811	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	65	theme	Asn-oligosaccharide	793:811	arg1	groups					783:788	the α-amino groups	771:788	the α-amino groups of Asn-oligosaccharide	771:811	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	1	66	dep	neoglycopolymers	233:248	arg1	first					310:314	first	310:314	first	310:314	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	66	dep	neoglycopolymers	233:248	arg1	second					361:366	second	361:366	second	361:366	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	66	dep	neoglycopolymers	233:248	arg1	third					414:418	third	414:418	third	414:418	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	2	67	theme	glycopeptide	713:724	arg1	digests					680:686	actinase digests	671:686	actinase digests of each glycoproteins or glycopeptide	671:724	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	68	gly	glycopeptides	652:664	arg2	glycopeptides					652:664	egg yolk glycopeptides	643:664	egg yolk glycopeptides	643:664	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	2	68	gly	glycopeptides	652:664	arg1	seeds					609:613	Ginkgo biloba seeds	595:613	Ginkgo biloba seeds	595:613	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	7	69	theme	multivalent	1497:1507	arg1	N-glycans					1527:1535	the multivalent high-mannose type N-glycans	1493:1535	the multivalent high-mannose type N-glycans	1493:1535	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	7	70	theme	bean	1631:1634	arg1	extract					1636:1642	jack bean extract	1626:1642	jack bean extract	1626:1642	Furthermore, we confirmed that the neoglycopolymer carrying the multivalent high-mannose type N-glycans is a useful tool for rapid purification of mannose-binding protein, Concanavalin A, from jack bean extract.					
31751705	1	71	theme	uncharacterized	143:157	arg1	receptors					174:182	uncharacterized glycan-binding receptors	143:182	uncharacterized glycan-binding receptors	143:182	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	72	with	second	361:366	arg1	M8					401:402	M8	401:402	M8	401:402	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	1	72	with	second	361:366	arg1	N-glycan					391:398	high-mannose type N-glycan	373:398	high-mannose type N-glycan (M8)	373:403	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	0	73	theme	N-glycopeptide	78:91	arg1	units					93:97	multivalent N-glycopeptide units	66:97	multivalent N-glycopeptide units	66:97	Synthesis and preliminary evaluation of neoglycopolymers carrying multivalent N-glycopeptide units.					
31751705	4	74	theme	reverse-phase	1066:1078	arg1	HPLC					1080:1083	reverse-phase HPLC	1066:1083	reverse-phase HPLC	1066:1083	The resulting neoglycopolymers were purified through a combination of gel-filtration and reverse-phase HPLC.					
31751705	2	75	theme	glycoproteins	696:708	arg1	digests					680:686	actinase digests	671:686	actinase digests of each glycoproteins or glycopeptide	671:724	Three types of Asn-oligosaccharide (Asn-M3FX, Asn-M8, or Asn-NeuAc2Gal2GN2M3) were prepared from storage glycoproteins of Ginkgo biloba seeds, Vigna angularis seeds, and egg yolk glycopeptides from actinase digests of each glycoproteins or glycopeptide.					
31751705	1	76	theme	glycan-binding	159:172	arg1	receptors					174:182	uncharacterized glycan-binding receptors	143:182	uncharacterized glycan-binding receptors	143:182	In the present study, for the discovery of uncharacterized glycan-binding receptors or lectin-like receptors in plants, we developed neoglycopolymers to which three types of N-glycopeptides are conjugated; the first with plant complex type N-glycan (M3FX), the second with high-mannose type N-glycan (M8), and the third with animal complex type N-glycan (NeuAc2Gal2GN2M3).					
31751705	3	77	theme	α-amino	775:781	arg1	Asn-oligosaccharide					793:811	Asn-oligosaccharide	793:811	Asn-oligosaccharide	793:811	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
31751705	3	77	theme	α-amino	775:781	arg1	groups					783:788	the α-amino groups	771:788	the α-amino groups of Asn-oligosaccharide	771:811	Neoglycopolymers were synthesized such that the α-amino groups of Asn-oligosaccharide were coupled to the carboxyl groups of poly-γ-L-glutamic acid (γ-L-PGA) with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM).					
29339133	5	0	from	LMWH	707:710	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	0	from	LMWH	707:710	arg1	affinity					671:678	antithrombin III-binding affinity	646:678	antithrombin III-binding affinity	646:678	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	0	from	LMWH	707:710	arg1	fractions					687:695	the fractions	683:695	the fractions from this LMWH	683:710	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	0	from	LMWH	707:710	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	4	1	theme	weight	507:512	arg1	fractions					514:522	different molecular weight fractions	487:522	different molecular weight fractions	487:522	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	4	2	dep	enoxaparin	419:428	arg1	sodium					430:435	sodium	430:435	enoxaparin sodium (a generic version of Lovenox)	419:466	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	6	3	theme	larger-sized	888:899	arg1	chains					901:906	larger-sized chains	888:906	larger-sized chains	888:906	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	6	4	theme	III-binding	932:942	arg1	affinity					944:951	higher antithrombin III-binding affinity	912:951	higher antithrombin III-binding affinity	912:951	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	4	5	theme	LMWH	413:416	arg1	enoxaparin					419:428	enoxaparin sodium (a generic version of Lovenox)	419:466	enoxaparin sodium (a generic version of Lovenox)	419:466	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	6	6	theme	3-O-sulfo	803:811	arg1	tetrasaccharide					830:844	3-O-sulfo group-containing tetrasaccharide	803:844	3-O-sulfo group-containing tetrasaccharide	803:844	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	5	7	theme	antithrombin	646:657	arg1	affinity					671:678	antithrombin III-binding affinity	646:678	antithrombin III-binding affinity	646:678	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	7	theme	antithrombin	646:657	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	1	8	theme	Low-molecular-weight	82:101	arg1	heparins					103:110	Low-molecular-weight heparins	82:110	Low-molecular-weight heparins (LMWHs)	82:118	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	8	theme	Low-molecular-weight	82:101	arg1	drugs					162:166	clinical anticoagulant drugs	139:166	clinical anticoagulant drugs	139:166	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	8	theme	Low-molecular-weight	82:101	arg1	LMWHs					113:117	LMWHs	113:117	LMWHs	113:117	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	6	9	contain	containing	877:886	arg1	fraction					868:875	the fraction	864:875	the fraction containing larger-sized chains	864:906	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	6	9	contain	containing	877:886	arg2	chains					901:906	larger-sized chains	888:906	larger-sized chains	888:906	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	3	10	theme	quality	378:384	arg1	control					386:392	the drug quality control	369:392	the drug quality control	369:392	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	5	11	theme	III-binding	659:669	arg1	affinity					671:678	antithrombin III-binding affinity	646:678	antithrombin III-binding affinity	646:678	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	11	theme	III-binding	659:669	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	4	12	theme	molecular	497:505	arg1	fractions					514:522	different molecular weight fractions	487:522	different molecular weight fractions	487:522	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	5	13	theme	3-O-sulfo	585:593	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	13	theme	3-O-sulfo	585:593	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	0	14	theme	III-Binding	13:23	arg1	Analysis					30:37	Antithrombin III-Binding Site Analysis	0:37	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.	0:80	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	4	15	theme	different	487:495	arg1	fractions					514:522	different molecular weight fractions	487:522	different molecular weight fractions	487:522	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	5	16	theme	group-containing	595:610	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	16	theme	group-containing	595:610	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	0	17	theme	Antithrombin	0:11	arg1	Analysis					30:37	Antithrombin III-Binding Site Analysis	0:37	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.	0:80	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	4	18	theme	generic	440:446	arg1	version					448:454	a generic version	438:454	a generic version of Lovenox	438:465	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	5	19	theme	tetrasaccharides	612:627	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	19	theme	tetrasaccharides	612:627	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	3	20	theme	structural	322:331	arg1	essential					355:363	essential	355:363	essential	355:363	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	3	20	theme	structural	322:331	arg1	analysis					333:340	The detailed structural analysis	309:340	The detailed structural analysis of a LMWH	309:350	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	6	21	theme	tetrasaccharide	830:844	arg1	compositions					846:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	5	22	from	compositions	571:582	arg1	LMWH					707:710	this LMWH	702:710	this LMWH	702:710	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	0	23	theme	Site	25:28	arg1	Analysis					30:37	Antithrombin III-Binding Site Analysis	0:37	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.	0:80	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	4	24	theme	Lovenox	459:465	arg1	version					448:454	a generic version	438:454	a generic version of Lovenox	438:465	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	1	25	used	used	131:134	arg2	LMWHs					113:117	LMWHs	113:117	LMWHs	113:117	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	25	used	used	131:134	arg2	drugs					162:166	clinical anticoagulant drugs	139:166	clinical anticoagulant drugs	139:166	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	25	used	used	131:134	arg2	heparins					103:110	Low-molecular-weight heparins	82:110	Low-molecular-weight heparins (LMWHs)	82:118	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	0	26	theme	Low-Molecular-Weight	42:61	arg1	Fractions					71:79	Low-Molecular-Weight Heparin Fractions	42:79	Low-Molecular-Weight Heparin Fractions	42:79	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	6	27	contain	had	769:771	arg1	fractions					759:767	all the fractions	751:767	all the fractions	751:767	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	6	27	contain	had	769:771	arg2	compositions					846:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	4	28	theme	peptide	538:544	arg1	column					546:551	a Superdex peptide column	527:551	a Superdex peptide column	527:551	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	3	29	theme	detailed	313:320	arg1	essential					355:363	essential	355:363	essential	355:363	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	3	29	theme	detailed	313:320	arg1	analysis					333:340	The detailed structural analysis	309:340	The detailed structural analysis of a LMWH	309:350	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	2	30	theme	enzymatic	255:263	arg1	depolymerization					265:280	chemical or enzymatic depolymerization	243:280	chemical or enzymatic depolymerization of unfractionated heparin	243:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	6	31	theme	group-containing	813:828	arg1	tetrasaccharide					830:844	3-O-sulfo group-containing tetrasaccharide	803:844	3-O-sulfo group-containing tetrasaccharide	803:844	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	0	32	theme	Fractions	71:79	arg1	Analysis					30:37	Antithrombin III-Binding Site Analysis	0:37	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.	0:80	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	4	33	theme	Superdex	529:536	arg1	column					546:551	a Superdex peptide column	527:551	a Superdex peptide column	527:551	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
29339133	3	34	theme	LMWH	347:350	arg1	essential					355:363	essential	355:363	essential	355:363	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	3	34	theme	LMWH	347:350	arg1	analysis					333:340	The detailed structural analysis	309:340	The detailed structural analysis of a LMWH	309:350	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	2	35	theme	heterogeneous	179:191	arg1	glycans					223:229	heterogeneous and highly negatively charged glycans	179:229	heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin	179:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	2	35	theme	heterogeneous	179:191	arg1	LMWHs					169:173	LMWHs	169:173	LMWHs	169:173	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	5	36	theme	disaccharide	558:569	arg1	affinity					671:678	antithrombin III-binding affinity	646:678	antithrombin III-binding affinity	646:678	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	36	theme	disaccharide	558:569	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	36	theme	disaccharide	558:569	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	0	37	theme	Heparin	63:69	arg1	Fractions					71:79	Low-Molecular-Weight Heparin Fractions	42:79	Low-Molecular-Weight Heparin Fractions	42:79	Antithrombin III-Binding Site Analysis of Low-Molecular-Weight Heparin Fractions.					
29339133	6	38	theme	antithrombin	919:930	arg1	affinity					944:951	higher antithrombin III-binding affinity	912:951	higher antithrombin III-binding affinity	912:951	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	2	39	theme	chemical	243:250	arg1	depolymerization					265:280	chemical or enzymatic depolymerization	243:280	chemical or enzymatic depolymerization of unfractionated heparin	243:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	5	40	theme	fractions	687:695	arg1	affinity					671:678	antithrombin III-binding affinity	646:678	antithrombin III-binding affinity	646:678	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	40	theme	fractions	687:695	arg1	composition					629:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	3-O-sulfo group-containing tetrasaccharides composition	585:639	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	5	40	theme	fractions	687:695	arg1	compositions					571:582	The disaccharide compositions	554:582	The disaccharide compositions	554:582	The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed.					
29339133	6	41	theme	higher	912:917	arg1	affinity					944:951	higher antithrombin III-binding affinity	912:951	higher antithrombin III-binding affinity	912:951	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	6	42	theme	disaccharide	786:797	arg1	compositions					846:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	1	43	theme	clinical	139:146	arg1	heparins					103:110	Low-molecular-weight heparins	82:110	Low-molecular-weight heparins (LMWHs)	82:118	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	43	theme	clinical	139:146	arg1	drugs					162:166	clinical anticoagulant drugs	139:166	clinical anticoagulant drugs	139:166	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	2	44	theme	heparin	300:306	arg1	depolymerization					265:280	chemical or enzymatic depolymerization	243:280	chemical or enzymatic depolymerization of unfractionated heparin	243:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	6	45	theme	similar	778:784	arg1	compositions					846:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions	773:857	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	1	46	theme	anticoagulant	148:160	arg1	heparins					103:110	Low-molecular-weight heparins	82:110	Low-molecular-weight heparins (LMWHs)	82:118	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	1	46	theme	anticoagulant	148:160	arg1	drugs					162:166	clinical anticoagulant drugs	139:166	clinical anticoagulant drugs	139:166	Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs.					
29339133	2	47	theme	charged	215:221	arg1	glycans					223:229	heterogeneous and highly negatively charged glycans	179:229	heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin	179:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	2	47	theme	charged	215:221	arg1	LMWHs					169:173	LMWHs	169:173	LMWHs	169:173	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	2	48	theme	unfractionated	285:298	arg1	heparin					300:306	unfractionated heparin	285:306	unfractionated heparin	285:306	LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin.					
29339133	3	49	theme	drug	373:376	arg1	control					386:392	the drug quality control	369:392	the drug quality control	369:392	The detailed structural analysis of a LMWH is essential for the drug quality control.					
29339133	6	50	contain	had	908:910	arg1	fraction					868:875	the fraction	864:875	the fraction containing larger-sized chains	864:906	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	6	50	contain	had	908:910	arg2	affinity					944:951	higher antithrombin III-binding affinity	912:951	higher antithrombin III-binding affinity	912:951	The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.					
29339133	4	51	dep	sodium	430:435	arg1	version					448:454	a generic version	438:454	a generic version of Lovenox	438:465	In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column.					
31116986	0	0	theme	HIV	67:69	arg1	Template					79:86	an HIV Vaccine Template	64:86	an HIV Vaccine Template	64:86	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	7	1	theme	sequential	1140:1149	arg1	regimen					1164:1170	a sequential immunization regimen	1138:1170	a sequential immunization regimen	1138:1170	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	1	2	theme	antibody	140:147	arg1	responses					149:157	antibody responses	140:157	antibody responses to broadly neutralizing antibody (bnAb) sites	140:203	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	6	3	theme	trimer	918:923	arg1	cocktail					925:932	an HIV trimer cocktail	911:932	an HIV trimer cocktail	911:932	Subsequent boosting with an HIV trimer cocktail induces responses that exhibit some virus cross-neutralization.					
31116986	2	4	theme	HIV immunization	403:418	arg1	strategy					420:427	an HIV immunization strategy	400:427	an HIV immunization strategy	400:427	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	3	5	theme	HIV	587:589	arg1	specificities					591:603	other HIV specificities	581:603	other HIV specificities	581:603	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	7	6	theme	V2-apex	1182:1188	arg1	bnAbs					1190:1194	V2-apex bnAbs	1182:1194	V2-apex bnAbs	1182:1194	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	6	7	theme	HIV	914:916	arg1	cocktail					925:932	an HIV trimer cocktail	911:932	an HIV trimer cocktail	911:932	Subsequent boosting with an HIV trimer cocktail induces responses that exhibit some virus cross-neutralization.					
31116986	3	8	theme	Env	465:467	arg1	MT145K					477:482	MT145K	477:482	MT145K	477:482	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	3	8	theme	Env	465:467	arg1	trimer					469:474	a chimpanzee SIV Env trimer	448:474	a chimpanzee SIV Env trimer	448:474	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	4	9	theme	trimer	647:652	arg1	structure					623:631	the structure	619:631	the structure of the MT145K trimer by cryo-EM	619:663	We determine the structure of the MT145K trimer by cryo-EM and show that its architecture is remarkably similar to HIV Env.					
31116986	0	10	theme	Vaccine	71:77	arg1	Template					79:86	an HIV Vaccine Template	64:86	an HIV Vaccine Template	64:86	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	2	11	from	utility	389:395	arg1	strategy					420:427	an HIV immunization strategy	400:427	an HIV immunization strategy	400:427	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	5	12	theme	mouse	798:802	arg1	Immunization					730:741	Immunization	730:741	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer	730:836	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	7	13	theme	potential	1115:1123	arg1	component					1125:1133	an attractive potential component	1101:1133	an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs	1101:1194	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	2	14	theme	bnAb	313:316	arg1	-apex					348:352	the variable loop 2 (V2)-apex	324:352	the variable loop 2 (V2)-apex	324:352	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	14	theme	bnAb	313:316	arg1	site					318:321	a single bnAb site	304:321	a single bnAb site	304:321	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	6	15	theme	virus	970:974	arg1	cross-neutralization					976:995	some virus cross-neutralization	965:995	some virus cross-neutralization	965:995	Subsequent boosting with an HIV trimer cocktail induces responses that exhibit some virus cross-neutralization.					
31116986	3	16	theme	chimpanzee	450:459	arg1	MT145K					477:482	MT145K	477:482	MT145K	477:482	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	3	16	theme	chimpanzee	450:459	arg1	trimer					469:474	a chimpanzee SIV Env trimer	448:474	a chimpanzee SIV Env trimer	448:474	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	5	17	theme	bnAb	761:764	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	2	18	theme	V2	345:346	arg1	-apex					348:352	the variable loop 2 (V2)-apex	324:352	the variable loop 2 (V2)-apex	324:352	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	18	theme	V2	345:346	arg1	site					318:321	a single bnAb site	304:321	a single bnAb site	304:321	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	19	theme	virus	270:274	arg1	Env					292:294	Env	292:294	Env	292:294	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	19	theme	virus	270:274	arg1	envelope					282:289	A chimpanzee simian immunodeficiency virus (SIV) envelope	233:289	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env)	233:295	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	1	20	theme	broadly	162:168	arg1	bnAb					193:196	bnAb	193:196	bnAb	193:196	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	1	20	theme	broadly	162:168	arg1	antibody					183:190	broadly neutralizing antibody	162:190	broadly neutralizing antibody (bnAb) sites	162:203	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	0	21	theme	Chimpanzee	4:13	arg1	Trimer					28:33	The Chimpanzee SIV Envelope Trimer	0:33	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.	0:87	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	7	22	theme	immunization	1151:1162	arg1	regimen					1164:1170	a sequential immunization regimen	1138:1170	a sequential immunization regimen	1138:1170	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	7	23	theme	chimpanzee	1011:1020	arg1	trimer					1029:1034	the chimpanzee MT145K trimer	1007:1034	the chimpanzee MT145K trimer	1007:1034	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	4	24	theme	MT145K	640:645	arg1	trimer					647:652	the MT145K trimer	636:652	the MT145K trimer	636:652	We determine the structure of the MT145K trimer by cryo-EM and show that its architecture is remarkably similar to HIV Env.					
31116986	1	25	theme	neutralizing	170:181	arg1	bnAb					193:196	bnAb	193:196	bnAb	193:196	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	1	25	theme	neutralizing	170:181	arg1	antibody					183:190	broadly neutralizing antibody	162:190	broadly neutralizing antibody (bnAb) sites	162:203	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	7	26	theme	attractive	1104:1113	arg1	component					1125:1133	an attractive potential component	1101:1133	an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs	1101:1194	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	7	27	theme	MT145K	1022:1027	arg1	trimer					1029:1034	the chimpanzee MT145K trimer	1007:1034	the chimpanzee MT145K trimer	1007:1034	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	2	28	theme	loop	337:340	arg1	-apex					348:352	the variable loop 2 (V2)-apex	324:352	the variable loop 2 (V2)-apex	324:352	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	28	theme	loop	337:340	arg1	site					318:321	a single bnAb site	304:321	a single bnAb site	304:321	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	0	29	theme	Envelope	19:26	arg1	Trimer					28:33	The Chimpanzee SIV Envelope Trimer	0:33	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.	0:87	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	3	30	theme	HIV	521:523	arg1	bnAbs					533:537	HIV V2-apex bnAbs	521:537	HIV V2-apex bnAbs	521:537	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	3	31	theme	selective	500:508	arg1	binding					510:516	selective binding	500:516	selective binding to HIV V2-apex bnAbs and precursor versions	500:560	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	5	32	theme	V2-specific	850:860	arg1	responses					875:883	HIV V2-specific neutralizing responses	846:883	HIV V2-specific neutralizing responses	846:883	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	5	33	theme	neutralizing	862:873	arg1	responses					875:883	HIV V2-specific neutralizing responses	846:883	HIV V2-specific neutralizing responses	846:883	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	0	34	theme	SIV	15:17	arg1	Trimer					28:33	The Chimpanzee SIV Envelope Trimer	0:33	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.	0:87	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	6	35	with	boosting	897:904	arg1	cocktail					925:932	an HIV trimer cocktail	911:932	an HIV trimer cocktail	911:932	Subsequent boosting with an HIV trimer cocktail induces responses that exhibit some virus cross-neutralization.					
31116986	1	36	theme	Epitope-targeted	89:104	arg1	design					118:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	5	37	theme	Ab-expressing	776:788	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	2	38	theme	single	306:311	arg1	-apex					348:352	the variable loop 2 (V2)-apex	324:352	the variable loop 2 (V2)-apex	324:352	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	38	theme	single	306:311	arg1	site					318:321	a single bnAb site	304:321	a single bnAb site	304:321	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	1	39	theme	antibody	183:190	arg1	sites					199:203	broadly neutralizing antibody (bnAb) sites	162:203	broadly neutralizing antibody (bnAb) sites	162:203	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	5	40	theme	HIV	749:751	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	5	41	with	Immunization	730:741	arg1	trimer					831:836	the chimpanzee MT145K trimer	809:836	the chimpanzee MT145K trimer	809:836	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	3	42	theme	precursor	543:551	arg1	versions					553:560	precursor versions	543:560	precursor versions	543:560	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	5	43	theme	chimpanzee	813:822	arg1	trimer					831:836	the chimpanzee MT145K trimer	809:836	the chimpanzee MT145K trimer	809:836	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	4	44	theme	HIV	721:723	arg1	Env					725:727	HIV Env	721:727	HIV Env	721:727	We determine the structure of the MT145K trimer by cryo-EM and show that its architecture is remarkably similar to HIV Env.					
31116986	6	45	theme	Subsequent	886:895	arg1	boosting					897:904	Subsequent boosting	886:904	Subsequent boosting with an HIV trimer cocktail	886:932	Subsequent boosting with an HIV trimer cocktail induces responses that exhibit some virus cross-neutralization.					
31116986	1	46	theme	HIV	106:108	arg1	design					118:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	2	47	theme	possible	380:387	arg1	utility					389:395	its possible utility	376:395	its possible utility in an HIV immunization strategy	376:427	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	0	48	dep	Trimer	28:33	arg1	Deployment					50:59	Deployment	50:59	Deployment	50:59	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	0	48	dep	Trimer	28:33	arg1	Structure					36:44	Structure	36:44	Structure	36:44	The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.					
31116986	1	49	theme	vaccine	110:116	arg1	design					118:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design	89:123	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	3	50	theme	other	581:585	arg1	specificities					591:603	other HIV specificities	581:603	other HIV specificities	581:603	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	2	51	theme	immunodeficiency	253:268	arg1	SIV					277:279	SIV	277:279	SIV	277:279	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	51	theme	immunodeficiency	253:268	arg1	virus					270:274	A chimpanzee simian immunodeficiency virus	233:274	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env)	233:295	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	5	52	theme	MT145K	824:829	arg1	trimer					831:836	the chimpanzee MT145K trimer	809:836	the chimpanzee MT145K trimer	809:836	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	5	53	theme	precursor	766:774	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	3	54	theme	SIV	461:463	arg1	MT145K					477:482	MT145K	477:482	MT145K	477:482	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	3	54	theme	SIV	461:463	arg1	trimer					469:474	a chimpanzee SIV Env trimer	448:474	a chimpanzee SIV Env trimer	448:474	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	2	55	theme	simian	246:251	arg1	SIV					277:279	SIV	277:279	SIV	277:279	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	55	theme	simian	246:251	arg1	virus					270:274	A chimpanzee simian immunodeficiency virus	233:274	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env)	233:295	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	1	56	theme	sequential	208:217	arg1	immunization					219:230	sequential immunization	208:230	sequential immunization	208:230	Epitope-targeted HIV vaccine design seeks to focus antibody responses to broadly neutralizing antibody (bnAb) sites by sequential immunization.					
31116986	7	57	theme	regimen	1164:1170	arg1	component					1125:1133	an attractive potential component	1101:1133	an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs	1101:1194	Overall, the chimpanzee MT145K trimer behaves as expected from design both in vitro and in vivo and is an attractive potential component of a sequential immunization regimen to induce V2-apex bnAbs.					
31116986	2	58	theme	chimpanzee	235:244	arg1	SIV					277:279	SIV	277:279	SIV	277:279	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	58	theme	chimpanzee	235:244	arg1	virus					270:274	A chimpanzee simian immunodeficiency virus	233:274	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env)	233:295	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	59	theme	variable	328:335	arg1	-apex					348:352	the variable loop 2 (V2)-apex	324:352	the variable loop 2 (V2)-apex	324:352	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	2	59	theme	variable	328:335	arg1	site					318:321	a single bnAb site	304:321	a single bnAb site	304:321	A chimpanzee simian immunodeficiency virus (SIV) envelope (Env) shares a single bnAb site, the variable loop 2 (V2)-apex, with HIV, suggesting its possible utility in an HIV immunization strategy.					
31116986	5	60	theme	V2-apex	753:759	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31116986	3	61	theme	V2-apex	525:531	arg1	bnAbs					533:537	HIV V2-apex bnAbs	521:537	HIV V2-apex bnAbs	521:537	Here, we generate a chimpanzee SIV Env trimer, MT145K, which displays selective binding to HIV V2-apex bnAbs and precursor versions, but no binding to other HIV specificities.					
31116986	5	62	theme	knockin	790:796	arg1	mouse					798:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	an HIV V2-apex bnAb precursor Ab-expressing knockin mouse	746:802	Immunization of an HIV V2-apex bnAb precursor Ab-expressing knockin mouse with the chimpanzee MT145K trimer induces HIV V2-specific neutralizing responses.					
31420336	4	0	theme	endo-xyloglucanases	913:931	arg1	Enzymology					836:845	Enzymology	836:845	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	836:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	7	1	dep	essential	1840:1848	arg1	understand					1858:1867	understand	1858:1867	to understand the ecology of these complex communities	1855:1908	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	7	1	dep	essential	1840:1848	arg1	manipulate					1917:1926	manipulate	1917:1926	manipulate	1917:1926	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	8	2	theme	structural	2153:2162	arg1	variations					2164:2173	the structural variations	2149:2173	the structural variations of plant xyloglucans	2149:2194	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	2	3	theme	functional	361:370	arg1	studies					372:378	functional studies	361:378	functional studies	361:378	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	4	4	theme	surprising	942:951	arg1	differences					953:963	surprising differences	942:963	surprising differences	942:963	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	4	4	theme	surprising	942:951	arg1	ability					1030:1036	the ability	1026:1036	the ability of some homologs to hydrolyze congested branched positions	1026:1095	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	6	5	dep	composition	1709:1719	arg1	the					1705:1707	the	1705:1707	the	1705:1707	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	3	6	theme	substrate	763:771	arg1	specificity					773:783	substrate specificity	763:783	substrate specificity	763:783	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	1	7	theme	dietary	300:306	arg1	fiber					308:312	dietary fiber	300:312	dietary fiber	300:312	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	6	8	theme	proteins	1431:1438	arg1	characterization					1357:1372	characterization	1357:1372	characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL	1357:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	9	theme	gut	1741:1743	arg1	microbiota					1745:1754	the gut microbiota	1737:1754	the gut microbiota	1737:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	4	10	theme	GH5_4	906:910	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	9	11	from	guts	2322:2325	arg1	prediction					2242:2251	refined functional prediction	2223:2251	refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2223:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	6	12	theme	surface	1408:1414	arg1	proteins					1431:1438	highly sequence-divergent cell surface glycan-binding proteins	1377:1438	highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL	1377:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	12	theme	surface	1408:1414	arg1	SGBPs					1441:1445	SGBPs	1441:1445	SGBPs	1441:1445	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	1	13	theme	individual	201:210	arg1	species					212:218	individual species	201:218	individual species	201:218	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	6	14	theme	XyG	1496:1498	arg1	SGBPs					1525:1529	XyG oligosaccharide-specific SGBPs	1496:1529	XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1496:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	2	15	theme	same	322:325	arg1	time					327:330	the same time	318:330	the same time	318:330	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	4	16	theme	cleavage	994:1001	arg1	specificity					1003:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	6	17	theme	cell	1403:1406	arg1	proteins					1431:1438	highly sequence-divergent cell surface glycan-binding proteins	1377:1438	highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL	1377:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	17	theme	cell	1403:1406	arg1	SGBPs					1441:1445	SGBPs	1441:1445	SGBPs	1441:1445	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	5	18	theme	alpha-l-arabinofuranosidases	1145:1172	arg1	complement					1126:1135	the complement	1122:1135	the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases	1122:1201	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	3	19	theme	utilization	656:666	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	19	theme	utilization	656:666	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	1	20	theme	gut	234:236	arg1	microbiota					238:247	animal gut microbiota	227:247	animal gut microbiota	227:247	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	3	21	theme	polysaccharide	808:821	arg1	chains					828:833	distinct polysaccharide side chains	799:833	distinct polysaccharide side chains	799:833	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	22	theme	syntenic	636:643	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	22	theme	syntenic	636:643	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	7	23	theme	systems	1829:1835	arg1	characterization					1782:1797	detailed molecular characterization	1763:1797	detailed molecular characterization of dietary glycan utilization systems	1763:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	8	24	theme	new	2000:2002	arg1	insight					2004:2010	new insight	2000:2010	new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans	2000:2194	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	7	25	theme	glycan	1810:1815	arg1	systems					1829:1835	dietary glycan utilization systems	1802:1835	dietary glycan utilization systems	1802:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	0	26	theme	Gut	60:62	arg1	Loci					46:49	Syntenic Xyloglucan Utilization Loci	14:49	Syntenic Xyloglucan Utilization Loci of Human Gut	14:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	4	27	theme	homologs	1046:1053	arg1	ability					1030:1036	the ability	1026:1036	the ability of some homologs to hydrolyze congested branched positions	1026:1095	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	5	28	theme	distinct	1231:1238	arg1	abilities					1240:1248	distinct abilities	1231:1248	distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan	1231:1345	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	8	29	theme	ubiquitous	2021:2030	arg1	members					2032:2038	ubiquitous members	2021:2038	ubiquitous members of the human gut microbiota	2021:2066	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	7	30	theme	molecular	1772:1780	arg1	characterization					1782:1797	detailed molecular characterization	1763:1797	detailed molecular characterization of dietary glycan utilization systems	1763:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	9	31	theme	functional	2231:2240	arg1	prediction					2242:2251	refined functional prediction	2223:2251	refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2223:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	0	32	theme	Side	96:99	arg1	Chain					101:105	Polysaccharide Side Chain	81:105	Polysaccharide Side Chain Diversity	81:115	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	5	33	theme	GH95	1178:1181	arg1	alpha-l-fucosidases					1183:1201	GH95 alpha-l-fucosidases	1178:1201	GH95 alpha-l-fucosidases	1178:1201	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	0	34	theme	Human	54:58	arg1	Gut					60:62	Human Gut	54:62	Human Gut	54:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	6	35	theme	SGBPs	1525:1529	arg1	SGBPs					1525:1529	XyG oligosaccharide-specific SGBPs	1496:1529	XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1496:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	35	theme	SGBPs	1525:1529	arg1	group					1487:1491	a novel group	1479:1491	a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1479:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	36	dep	BacteroidesIMPORTANCE	1553:1573	arg1	drives					1698:1703	drives	1698:1703	drives the composition and function of the gut microbiota	1698:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	8	37	theme	human	2047:2051	arg1	microbiota					2057:2066	the human gut microbiota	2043:2066	the human gut microbiota	2043:2066	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	7	38	theme	communities	1898:1908	arg1	ecology					1873:1879	the ecology	1869:1879	the ecology of these complex communities	1869:1908	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	9	39	theme	utilization	2267:2277	arg1	prediction					2242:2251	refined functional prediction	2223:2251	refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2223:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	6	40	theme	endogenous	1641:1650	arg1	enzymes					1662:1668	the endogenous digestive enzymes	1637:1668	the endogenous digestive enzymes of humans and other animals	1637:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	5	41	theme	species-specific	1276:1291	arg1	arabinogalactoxyloglucan					1293:1316	plant species-specific arabinogalactoxyloglucan	1270:1316	plant species-specific arabinogalactoxyloglucan	1270:1316	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	8	42	theme	microbiota	2057:2066	arg1	members					2032:2038	ubiquitous members	2021:2038	ubiquitous members of the human gut microbiota	2021:2066	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	2	43	theme	gut	457:459	arg1	composition					472:482	the gut microbiota composition	453:482	the gut microbiota composition	453:482	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	9	44	theme	diverse	2285:2291	arg1	taxa					2307:2310	diverse environmental taxa	2285:2310	diverse environmental taxa	2285:2310	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	0	45	theme	Syntenic	14:21	arg1	Loci					46:49	Syntenic Xyloglucan Utilization Loci	14:49	Syntenic Xyloglucan Utilization Loci of Human Gut	14:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	4	46	theme	hydrolase	874:882	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	2	47	theme	glycan	428:433	arg1	structures					435:444	dietary glycan structures	420:444	dietary glycan structures	420:444	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	0	48	theme	Utilization	34:44	arg1	Loci					46:49	Syntenic Xyloglucan Utilization Loci	14:49	Syntenic Xyloglucan Utilization Loci of Human Gut	14:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	4	49	theme	branched	1078:1085	arg1	positions					1087:1095	congested branched positions	1068:1095	congested branched positions	1068:1095	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	8	50	theme	plant	2178:2182	arg1	xyloglucans					2184:2194	plant xyloglucans	2178:2194	plant xyloglucans	2178:2194	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	6	51	theme	complex	1593:1599	arg1	carbohydrates					1601:1613	complex carbohydrates	1593:1613	complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals	1593:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	2	52	theme	limited	345:351	arg1	body					353:356	a currently limited body	333:356	a currently limited body of functional studies	333:378	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	9	53	from	utilization	2267:2277	arg1	beyond					2331:2336	beyond	2331:2336	beyond	2331:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	9	53	from	utilization	2267:2277	arg1	guts					2322:2325	animal guts	2315:2325	animal guts	2315:2325	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	3	54	theme	Dysgonomonas	710:721	arg1	species					723:729	three Bacteroides and one Dysgonomonas species	684:729	species	723:729	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	4	55	theme	syntenic	855:862	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	6	56	theme	other	1684:1688	arg1	animals					1690:1696	other animals	1684:1696	other animals	1684:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	2	57	theme	community	488:496	arg1	dynamics					498:505	community dynamics	488:505	community dynamics	488:505	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	9	58	from	beyond	2331:2336	arg1	prediction					2242:2251	refined functional prediction	2223:2251	refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2223:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	5	59	from	differences	1107:1117	arg1	complement					1126:1135	the complement	1122:1135	the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases	1122:1201	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	6	60	theme	syntenic	1455:1462	arg1	XyGUL					1464:1468	syntenic XyGUL	1455:1468	syntenic XyGUL	1455:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	2	61	theme	studies	372:378	arg1	body					353:356	a currently limited body	333:356	a currently limited body of functional studies	333:378	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	6	62	theme	humans	1673:1678	arg1	enzymes					1662:1668	the endogenous digestive enzymes	1637:1668	the endogenous digestive enzymes of humans and other animals	1637:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	1	63	theme	complex	267:273	arg1	carbohydrates					275:287	the complex carbohydrates	263:287	the complex carbohydrates comprising dietary fiber	263:312	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	8	64	theme	clusters	2114:2121	arg1	set					2083:2085	a set	2081:2085	a set of microheterogeneous gene clusters	2081:2121	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	8	64	theme	clusters	2114:2121	arg1	clusters					2114:2121	microheterogeneous gene clusters	2090:2121	microheterogeneous gene clusters	2090:2121	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	9	65	theme	animal	2315:2320	arg1	guts					2322:2325	animal guts	2315:2325	animal guts	2315:2325	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	3	66	theme	human	740:744	arg1	gut					746:748	the human gut	736:748	the human gut	736:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	8	67	theme	microheterogeneous	2090:2107	arg1	clusters					2114:2121	microheterogeneous gene clusters	2090:2121	microheterogeneous gene clusters	2090:2121	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	6	68	theme	microbiota	1745:1754	arg1	function					1725:1732	function	1725:1732	function	1725:1732	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	68	theme	microbiota	1745:1754	arg1	composition					1709:1719	composition	1709:1719	composition	1709:1719	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	1	69	theme	predicted	178:186	arg1	abilities					188:196	the predicted abilities	174:196	the predicted abilities of individual species	174:218	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	4	70	theme	subfamily	893:901	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	6	71	theme	glycan-binding	1416:1429	arg1	proteins					1431:1438	highly sequence-divergent cell surface glycan-binding proteins	1377:1438	highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL	1377:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	71	theme	glycan-binding	1416:1429	arg1	SGBPs					1441:1445	SGBPs	1441:1445	SGBPs	1441:1445	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	3	72	theme	recombinant	610:620	arg1	proteins					622:629	recombinant proteins	610:629	recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	610:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	8	73	theme	gene	2109:2112	arg1	clusters					2114:2121	microheterogeneous gene clusters	2090:2121	microheterogeneous gene clusters	2090:2121	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	3	74	theme	distinct	799:806	arg1	chains					828:833	distinct polysaccharide side chains	799:833	distinct polysaccharide side chains	799:833	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	6	75	theme	oligosaccharide-specific	1500:1523	arg1	SGBPs					1525:1529	XyG oligosaccharide-specific SGBPs	1496:1529	XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1496:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	1	76	theme	species	212:218	arg1	abilities					188:196	the predicted abilities	174:196	the predicted abilities of individual species	174:218	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	4	77	theme	XyG	980:982	arg1	specificity					1003:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	5	78	theme	GH43	1140:1143	arg1	alpha-l-arabinofuranosidases					1145:1172	GH43 alpha-l-arabinofuranosidases	1140:1172	GH43 alpha-l-arabinofuranosidases	1140:1172	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	3	79	theme	xyloglucan	645:654	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	79	theme	xyloglucan	645:654	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	1	80	theme	animal	227:232	arg1	microbiota					238:247	animal gut microbiota	227:247	animal gut microbiota	227:247	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	4	81	theme	backbone	985:992	arg1	specificity					1003:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	6	82	theme	sequence-divergent	1384:1401	arg1	proteins					1431:1438	highly sequence-divergent cell surface glycan-binding proteins	1377:1438	highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL	1377:1468	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	82	theme	sequence-divergent	1384:1401	arg1	SGBPs					1441:1445	SGBPs	1441:1445	SGBPs	1441:1445	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	3	83	from	gut	746:748	arg1	Bacteroides					690:700	three Bacteroides and one Dysgonomonas species	684:729	Bacteroides	690:700	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	83	from	gut	746:748	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	83	from	gut	746:748	arg1	species					723:729	three Bacteroides and one Dysgonomonas species	684:729	species	723:729	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	83	from	gut	746:748	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	6	84	theme	select	1546:1551	arg1	BacteroidesIMPORTANCE					1553:1573	select BacteroidesIMPORTANCE	1546:1573	select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1546:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	85	theme	novel	1481:1485	arg1	SGBPs					1525:1529	XyG oligosaccharide-specific SGBPs	1496:1529	XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1496:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	6	85	theme	novel	1481:1485	arg1	group					1487:1491	a novel group	1479:1491	a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota	1479:1754	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	1	86	theme	Genome	118:123	arg1	sequencing					125:134	Genome sequencing	118:134	Genome sequencing	118:134	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	7	87	theme	utilization	1817:1827	arg1	systems					1829:1835	dietary glycan utilization systems	1802:1835	dietary glycan utilization systems	1802:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	3	88	theme	side	823:826	arg1	chains					828:833	distinct polysaccharide side chains	799:833	distinct polysaccharide side chains	799:833	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	7	89	theme	human	1965:1969	arg1	health					1971:1976	human health	1965:1976	human health	1965:1976	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	7	90	theme	dietary	1802:1808	arg1	systems					1829:1835	dietary glycan utilization systems	1802:1835	dietary glycan utilization systems	1802:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	4	91	theme	xyloglucan	968:977	arg1	specificity					1003:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	8	92	theme	xyloglucans	2184:2194	arg1	variations					2164:2173	the structural variations	2149:2173	the structural variations of plant xyloglucans	2149:2194	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	4	93	from	differences	953:963	arg1	specificity					1003:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	xyloglucan (XyG) backbone cleavage specificity	968:1013	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	1	94	theme	substantial	149:159	arg1	variation					161:169	substantial variation	149:169	substantial variation in the predicted abilities of individual species within animal gut microbiota	149:247	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	0	95	theme	Polysaccharide	81:94	arg1	Chain					101:105	Polysaccharide Side Chain	81:105	Polysaccharide Side Chain Diversity	81:115	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	7	96	theme	detailed	1763:1770	arg1	characterization					1782:1797	detailed molecular characterization	1763:1797	detailed molecular characterization of dietary glycan utilization systems	1763:1835	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	9	97	theme	refined	2223:2229	arg1	prediction					2242:2251	refined functional prediction	2223:2251	refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2223:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	0	98	theme	Chain	101:105	arg1	Diversity					107:115	Polysaccharide Side Chain Diversity	81:115	Polysaccharide Side Chain Diversity	81:115	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	3	99	from	loci	668:671	arg1	gut					746:748	the human gut	736:748	the human gut	736:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	99	from	loci	668:671	arg1	proteins					622:629	recombinant proteins	610:629	recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	610:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	5	100	theme	alpha-l-fucosidases	1183:1201	arg1	complement					1126:1135	the complement	1122:1135	the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases	1122:1201	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	1	101	from	variation	161:169	arg1	abilities					188:196	the predicted abilities	174:196	the predicted abilities of individual species	174:218	Genome sequencing has revealed substantial variation in the predicted abilities of individual species within animal gut microbiota to metabolize the complex carbohydrates comprising dietary fiber.					
31420336	9	102	theme	environmental	2293:2305	arg1	taxa					2307:2310	diverse environmental taxa	2285:2310	diverse environmental taxa	2285:2310	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	9	103	theme	xyloglucan	2256:2265	arg1	utilization					2267:2277	xyloglucan utilization	2256:2277	xyloglucan utilization among diverse environmental taxa in animal guts and beyond	2256:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	6	104	theme	digestive	1652:1660	arg1	enzymes					1662:1668	the endogenous digestive enzymes	1637:1668	the endogenous digestive enzymes of humans and other animals	1637:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	5	105	theme	syntenic	1209:1216	arg1	XyGUL					1218:1222	syntenic XyGUL	1209:1222	syntenic XyGUL	1209:1222	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	2	106	theme	microbiota	461:470	arg1	composition					472:482	the gut microbiota composition	453:482	the gut microbiota composition	453:482	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	2	107	theme	richer	392:397	arg1	understanding					399:411	a richer understanding	390:411	a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics	390:505	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	0	108	theme	Xyloglucan	23:32	arg1	Loci					46:49	Syntenic Xyloglucan Utilization Loci	14:49	Syntenic Xyloglucan Utilization Loci of Human Gut	14:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	8	109	theme	gut	2053:2055	arg1	microbiota					2057:2066	the human gut microbiota	2043:2066	the human gut microbiota	2043:2066	Our research reveals new insight into how ubiquitous members of the human gut microbiota have evolved a set of microheterogeneous gene clusters to efficiently respond to the structural variations of plant xyloglucans.					
31420336	7	110	theme	complex	1890:1896	arg1	communities					1898:1908	these complex communities	1884:1908	these complex communities	1884:1908	Thus, detailed molecular characterization of dietary glycan utilization systems is essential both to understand the ecology of these complex communities and to manipulate their compositions, e.g., to benefit human health.					
31420336	3	111	theme	Bacteroides	690:700	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	111	theme	Bacteroides	690:700	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	0	112	theme	Loci	46:49	arg1	Adaptation					0:9	Adaptation	0:9	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut	0:62	Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity.					
31420336	4	113	theme	glycoside	864:872	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	2	114	theme	dietary	420:426	arg1	structures					435:444	dietary glycan structures	420:444	dietary glycan structures	420:444	At the same time, a currently limited body of functional studies precludes a richer understanding of how dietary glycan structures affect the gut microbiota composition and community dynamics.					
31420336	6	115	theme	carbohydrates	1601:1613	arg1	catabolism					1579:1588	The catabolism	1575:1588	The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals	1575:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	4	116	theme	congested	1068:1076	arg1	positions					1087:1095	congested branched positions	1068:1095	congested branched positions	1068:1095	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	3	117	theme	species	723:729	arg1	XyGUL					674:678	XyGUL	674:678	XyGUL	674:678	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	117	theme	species	723:729	arg1	loci					668:671	syntenic xyloglucan utilization loci	636:671	syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut	636:748	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	4	118	theme	family	884:889	arg1	endo-xyloglucanases					913:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases	850:931	Enzymology of four syntenic glycoside hydrolase family 5 subfamily 4 (GH5_4) endo-xyloglucanases revealed surprising differences in xyloglucan (XyG) backbone cleavage specificity, including the ability of some homologs to hydrolyze congested branched positions.					
31420336	3	119	theme	biophysical	536:546	arg1	techniques					548:557	biochemical and biophysical techniques	520:557	biochemical and biophysical techniques	520:557	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	3	120	theme	biochemical	520:530	arg1	techniques					548:557	biochemical and biophysical techniques	520:557	biochemical and biophysical techniques	520:557	Here, using biochemical and biophysical techniques, we identified and characterized differences among recombinant proteins from syntenic xyloglucan utilization loci (XyGUL) of three Bacteroides and one Dysgonomonas species from the human gut, which drive substrate specificity and access to distinct polysaccharide side chains.					
31420336	5	121	theme	plant	1270:1274	arg1	arabinogalactoxyloglucan					1293:1316	plant species-specific arabinogalactoxyloglucan	1270:1316	plant species-specific arabinogalactoxyloglucan	1270:1316	Further, differences in the complement of GH43 alpha-l-arabinofuranosidases and GH95 alpha-l-fucosidases among syntenic XyGUL confer distinct abilities to fully saccharify plant species-specific arabinogalactoxyloglucan and/or fucogalactoxyloglucan.					
31420336	6	122	theme	animals	1690:1696	arg1	enzymes					1662:1668	the endogenous digestive enzymes	1637:1668	the endogenous digestive enzymes of humans and other animals	1637:1696	Finally, characterization of highly sequence-divergent cell surface glycan-binding proteins (SGBPs) across syntenic XyGUL revealed a novel group of XyG oligosaccharide-specific SGBPs encoded within select BacteroidesIMPORTANCE The catabolism of complex carbohydrates that otherwise escape the endogenous digestive enzymes of humans and other animals drives the composition and function of the gut microbiota.					
31420336	9	123	from	prediction	2242:2251	arg1	beyond					2331:2336	beyond	2331:2336	beyond	2331:2336	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
31420336	9	123	from	prediction	2242:2251	arg1	guts					2322:2325	animal guts	2315:2325	animal guts	2315:2325	The data here will enable refined functional prediction of xyloglucan utilization among diverse environmental taxa in animal guts and beyond.					
29579191	6	0	theme	GTf	946:948	arg1	genes					950:954	GTf genes	946:954	GTf genes	946:954	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	7	1	dep	little	1148:1153	arg1	overlap					1155:1161	overlap	1155:1161	overlap between the two groups of genes	1155:1193	To resolve this issue, we surveyed the GTf-genome for reported CDGs and GWAS candidates; we found little overlap between the two groups of genes.					
29579191	8	2	theme	RNAseq	1471:1476	arg1	data					1478:1481	RNAseq data	1471:1481	RNAseq data	1471:1481	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	8	3	theme	transcriptional	1428:1442	arg1	regulation					1444:1453	(iii) transcriptional regulation as evaluated by RNAseq data	1422:1481	(iii) transcriptional regulation as evaluated by RNAseq data	1422:1481	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	1	4	theme	glycan	371:376	arg1	structures					378:387	glycan structures	371:387	glycan structures	371:387	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	7	5	theme	GWAS	1122:1125	arg1	candidates					1127:1136	GWAS candidates	1122:1136	GWAS candidates	1122:1136	To resolve this issue, we surveyed the GTf-genome for reported CDGs and GWAS candidates; we found little overlap between the two groups of genes.					
29579191	1	6	theme	lipids	190:195	arg1	Glycosylation					163:175	Glycosylation	163:175	Glycosylation of proteins, lipids and proteoglycans in human cells	163:228	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	6	7	theme	large	1006:1010	arg1	number					1012:1017	the large number	1002:1017	the large number of GWAS pointing to GTf-genes	1002:1047	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	9	8	theme	biological	1725:1734	arg1	functions					1736:1744	important biological functions	1715:1744	important biological functions	1715:1744	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	1	9	theme	structures	378:387	arg1	types					342:346	diverse types	334:346	diverse types of glycoconjugates and glycan structures	334:387	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	7	10	theme	reported	1104:1111	arg1	CDGs					1113:1116	reported CDGs	1104:1116	reported CDGs	1104:1116	To resolve this issue, we surveyed the GTf-genome for reported CDGs and GWAS candidates; we found little overlap between the two groups of genes.					
29579191	3	11	theme	large	569:573	arg1	number					575:580	a large number	567:580	a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS)	567:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	8	12	dep	regulation	1444:1453	arg1	iii					1423:1425	iii	1423:1425	iii	1423:1425	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	0	13	theme	glycosyltransferase	103:121	arg1	genes					123:127	glycosyltransferase genes	103:127	glycosyltransferase genes associated with complex diseases	103:160	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	6	14	theme	structural	924:933	arg1	defects					935:941	structural defects	924:941	structural defects in GTf genes	924:954	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	8	15	from	potential	1356:1364	arg1	pathways					1341:1348	glycosylation pathways	1327:1348	glycosylation pathways	1327:1348	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	5	16	theme	distinct	880:887	arg1	traits					889:894	less severe and distinct traits	864:894	less severe and distinct traits	864:894	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	5	17	theme	genetic	823:829	arg1	variations					831:840	more common genetic variations	811:840	more common genetic variations	811:840	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	6	18	with	odds	992:995	arg1	number					1012:1017	the large number	1002:1017	the large number of GWAS pointing to GTf-genes	1002:1047	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	2	19	theme	genome-the	420:429	arg1	GTf-genome-cause					431:446	the genome-the GTf-genome-cause	416:446	the genome-the GTf-genome-cause	416:446	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	2	20	dep	rare	461:464	arg1	monogenic					467:475	monogenic	467:475	monogenic	467:475	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	2	21	from	Mutations	390:398	arg1	part					408:411	this part	403:411	this part of the genome-the GTf-genome-cause	403:446	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	8	22	theme	predicted	1308:1316	arg1	roles					1318:1322	their: (i) predicted roles	1297:1322	their: (i) predicted roles in glycosylation pathways	1297:1348	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	1	23	theme	proteoglycans	201:213	arg1	Glycosylation					163:175	Glycosylation	163:175	Glycosylation of proteins, lipids and proteoglycans in human cells	163:228	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	2	24	theme	congenital	477:486	arg1	CDGs					516:519	CDGs	516:519	CDGs	516:519	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	2	24	theme	congenital	477:486	arg1	disorders					488:496	58 rare, monogenic congenital disorders	458:496	58 rare, monogenic congenital disorders of glycosylation (CDGs)	458:520	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	9	25	theme	important	1715:1723	arg1	functions					1736:1744	important biological functions	1715:1744	important biological functions	1715:1744	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	9	26	theme	glycosylation	1626:1638	arg1	reactions					1640:1648	some glycosylation reactions	1621:1648	some glycosylation reactions	1621:1648	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	1	27	from	Glycosylation	163:175	arg1	cells					224:228	human cells	218:228	human cells	218:228	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	3	28	theme	phenotypes	593:602	arg1	number					575:580	a large number	567:580	a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS)	567:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	0	29	theme	Glycosyltransferase	0:18	arg1	genes					20:24	Glycosyltransferase genes	0:24	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation	0:83	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	9	30	theme	complex	1515:1521	arg1	traits					1523:1528	more complex traits	1510:1528	more complex traits	1510:1528	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	1	31	theme	human	218:222	arg1	cells					224:228	human cells	218:228	human cells	218:228	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	8	32	from	regulation	1444:1453	arg1	pathways					1341:1348	glycosylation pathways	1327:1348	glycosylation pathways	1327:1348	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	9	33	theme	functions	1736:1744	arg1	fine-tuning					1700:1710	fine-tuning	1700:1710	fine-tuning of important biological functions	1700:1744	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	3	34	theme	predispositions	617:631	arg1	number					575:580	a large number	567:580	a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS)	567:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	8	35	dep	roles	1318:1322	arg1	i					1305:1305	i	1305:1305	i	1305:1305	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	2	36	theme	rare	461:464	arg1	CDGs					516:519	CDGs	516:519	CDGs	516:519	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	2	36	theme	rare	461:464	arg1	disorders					488:496	58 rare, monogenic congenital disorders	458:496	58 rare, monogenic congenital disorders of glycosylation (CDGs)	458:520	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	0	37	theme	complex	145:151	arg1	diseases					153:160	complex diseases	145:160	complex diseases	145:160	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	8	38	theme	homologous	1400:1409	arg1	genes					1411:1415	closely homologous genes	1392:1415	closely homologous genes	1392:1415	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	3	39	theme	complex	585:591	arg1	phenotypes					593:602	complex phenotypes	585:602	complex phenotypes	585:602	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	0	40	theme	congenital	47:56	arg1	disorders					58:66	monogenic congenital disorders	37:66	monogenic congenital disorders of glycosylation	37:83	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	1	41	gly	Glycosylation	163:175	arg1	proteins					180:187	proteins	180:187	proteins	180:187	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	1	41	gly	Glycosylation	163:175	arg1	proteoglycans					201:213	proteoglycans	201:213	proteoglycans	201:213	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	1	41	gly	Glycosylation	163:175	arg1	lipids					190:195	lipids	190:195	lipids	190:195	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	5	42	from	contrast	773:780	arg1	likely					792:797	likely	792:797	likely	792:797	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	2	43	dep	more	448:451	arg1	Mutations					390:398	Mutations	390:398	Mutations in this part of the genome-the GTf-genome-cause	390:446	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	5	44	theme	severe	869:874	arg1	traits					889:894	less severe and distinct traits	864:894	less severe and distinct traits	864:894	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	2	45	theme	glycosylation	501:513	arg1	CDGs					516:519	CDGs	516:519	CDGs	516:519	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	2	45	theme	glycosylation	501:513	arg1	disorders					488:496	58 rare, monogenic congenital disorders	458:496	58 rare, monogenic congenital disorders of glycosylation (CDGs)	458:520	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	0	46	theme	monogenic	37:45	arg1	disorders					58:66	monogenic congenital disorders	37:66	monogenic congenital disorders of glycosylation	37:83	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	3	47	theme	Genome-Wide	662:672	arg1	Studies					686:692	Genome-Wide Association Studies	662:692	Genome-Wide Association Studies (GWAS)	662:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	3	47	theme	Genome-Wide	662:672	arg1	GWAS					695:698	GWAS	695:698	GWAS	695:698	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	9	48	theme	GTfs	1595:1598	arg1	deficiency					1581:1590	structural deficiency	1570:1590	dysregulation rather than structural deficiency of GTfs	1544:1598	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	2	49	theme	GTf-genome-cause	431:446	arg1	part					408:411	this part	403:411	this part of the genome-the GTf-genome-cause	403:446	Mutations in this part of the genome-the GTf-genome-cause more than 58 rare, monogenic congenital disorders of glycosylation (CDGs).					
29579191	1	50	theme	diverse	334:340	arg1	types					342:346	diverse types	334:346	diverse types of glycoconjugates and glycan structures	334:387	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	8	51	theme	glycosylation	1327:1339	arg1	pathways					1341:1348	glycosylation pathways	1327:1348	glycosylation pathways	1327:1348	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	3	52	theme	Association	674:684	arg1	Studies					686:692	Genome-Wide Association Studies	662:692	Genome-Wide Association Studies (GWAS)	662:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	3	52	theme	Association	674:684	arg1	GWAS					695:698	GWAS	695:698	GWAS	695:698	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	3	53	theme	diseases	605:612	arg1	number					575:580	a large number	567:580	a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS)	567:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	5	54	theme	common	816:821	arg1	variations					831:840	more common genetic variations	811:840	more common genetic variations	811:840	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	1	55	theme	types	342:346	arg1	biosynthesis					318:329	the biosynthesis	314:329	the biosynthesis of diverse types of glycoconjugates and glycan structures	314:387	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	8	56	theme	different	1263:1271	arg1	classes					1273:1279	different classes	1263:1279	different classes	1263:1279	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	8	57	from	roles	1318:1322	arg1	pathways					1341:1348	glycosylation pathways	1327:1348	glycosylation pathways	1327:1348	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	6	58	from	defects	935:941	arg1	genes					950:954	GTf genes	946:954	GTf genes	946:954	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	9	59	theme	tight	1679:1683	arg1	regulation					1685:1694	tight regulation	1679:1694	tight regulation	1679:1694	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	5	60	from	likely	792:797	arg1	contrast					773:780	contrast	773:780	contrast	773:780	In contrast, GWAS are likely to identify more common genetic variations and generally involve less severe and distinct traits.					
29579191	8	61	theme	partial	1370:1376	arg1	redundancy					1378:1387	partial redundancy	1370:1387	partial redundancy by closely homologous genes	1370:1415	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	4	62	theme	medical	748:754	arg1	consequences					756:767	severe medical consequences	741:767	severe medical consequences	741:767	CDGs are extremely rare and often with severe medical consequences.					
29579191	0	63	from	genes	123:127	arg1	distinct					89:96	distinct	89:96	distinct	89:96	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	9	64	theme	structural	1570:1579	arg1	deficiency					1581:1590	structural deficiency	1570:1590	dysregulation rather than structural deficiency of GTfs	1544:1598	Our analysis suggest that more complex traits are caused by dysregulation rather than structural deficiency of GTfs, which suggests that some glycosylation reactions may be predicted to be under tight regulation for fine-tuning of important biological functions.					
29579191	4	65	theme	severe	741:746	arg1	consequences					756:767	severe medical consequences	741:767	severe medical consequences	741:767	CDGs are extremely rare and often with severe medical consequences.					
29579191	1	66	theme	identified	252:261	arg1	glycosyltransferases					263:282	at least 167 identified glycosyltransferases	239:282	at least 167 identified glycosyltransferases (GTfs)	239:289	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	1	66	theme	identified	252:261	arg1	GTfs					285:288	GTfs	285:288	GTfs	285:288	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	3	67	theme	complex	636:642	arg1	diseases					644:651	complex diseases	636:651	complex diseases based on Genome-Wide Association Studies (GWAS)	636:699	They are also statistically associated with a large number of complex phenotypes, diseases or predispositions to complex diseases based on Genome-Wide Association Studies (GWAS).					
29579191	8	68	dep	potential	1356:1364	arg1	ii					1352:1353	ii	1352:1353	ii	1352:1353	Moreover, GTf-genes implicated by CDG or GWAS appear to constitute different classes with respect to their: (i) predicted roles in glycosylation pathways; (ii) potential for partial redundancy by closely homologous genes; and (iii) transcriptional regulation as evaluated by RNAseq data.					
29579191	6	69	theme	GWAS	1022:1025	arg1	number					1012:1017	the large number	1002:1017	the large number of GWAS pointing to GTf-genes	1002:1047	We recently confirmed that structural defects in GTf genes are extremely rare, which seemed at odds with the large number of GWAS pointing to GTf-genes.					
29579191	0	70	theme	glycosylation	71:83	arg1	disorders					58:66	monogenic congenital disorders	37:66	monogenic congenital disorders of glycosylation	37:83	Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases.					
29579191	1	71	theme	glycoconjugates	351:365	arg1	types					342:346	diverse types	334:346	diverse types of glycoconjugates and glycan structures	334:387	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	1	72	theme	proteins	180:187	arg1	Glycosylation					163:175	Glycosylation	163:175	Glycosylation of proteins, lipids and proteoglycans in human cells	163:228	Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures.					
29579191	7	73	theme	genes	1189:1193	arg1	genes					1189:1193	genes	1189:1193	genes	1189:1193	To resolve this issue, we surveyed the GTf-genome for reported CDGs and GWAS candidates; we found little overlap between the two groups of genes.					
29579191	7	73	theme	genes	1189:1193	arg1	groups					1179:1184	the two groups	1171:1184	the two groups of genes	1171:1193	To resolve this issue, we surveyed the GTf-genome for reported CDGs and GWAS candidates; we found little overlap between the two groups of genes.					
30999248	8	0	theme	glycan	978:983	arg1	identification					995:1008	glycan structure identification	978:1008	glycan structure identification using tree merging strategy	978:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	13	1	theme	invalid	1846:1852	arg1	structures					1854:1863	invalid structures	1846:1863	invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency	1846:1972	Unlike the traditional enumerating followed by filtering strategy, our approach performed deisomorphism to remove isomorphic subtrees, and ruled out invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency.					
30999248	5	2	theme	glycan	487:492	arg1	identification					505:518	glycan structural identification	487:518	glycan structural identification from mass spectra	487:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	8	3	theme	tree	1016:1019	arg1	strategy					1029:1036	tree merging strategy	1016:1036	tree merging strategy	1016:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	15	4	from	identification	2176:2189	arg1	minutes					2194:2200	minutes	2194:2200	minutes	2194:2200	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	7	5	theme	mass	867:870	arg1	spectra					872:878	mass spectra	867:878	mass spectra	867:878	Thus, how to reliably and efficiently identify glycan structures from mass spectra still remains challenging.					
30999248	5	6	theme	de	465:466	arg1	approaches					473:482	Most de novo approaches	460:482	Most de novo approaches to glycan structural identification from mass spectra	460:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	5	7	from	spectra	530:536	arg1	identification					505:518	glycan structural identification	487:518	glycan structural identification from mass spectra	487:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	3	8	theme	fine	275:278	arg1	details					280:286	the fine details	271:286	the fine details of their structures	271:306	These roles are primarily determined by the fine details of their structures, making glycan structural identification highly desirable.					
30999248	15	9	theme	standards	2128:2136	arg1	standards					2128:2136	10 N-glycan standards	2116:2136	10 N-glycan standards	2116:2136	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	15	9	theme	standards	2128:2136	arg1	set					2109:2111	a test set	2102:2111	a test set of 10 N-glycan standards	2102:2136	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	9	10	theme	underlying	1252:1261	arg1	structure					1275:1283	the underlying glycan tree structure	1248:1283	the underlying glycan tree structure	1248:1283	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	15	11	theme	highest	2256:2262	arg1	score					2264:2268	the manually-validated structure first three highest score	2211:2268	the manually-validated structure first three highest score	2211:2268	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	4	12	theme	glycan	441:446	arg1	structures					448:457	glycan structures	441:457	glycan structures	441:457	Mass spectrometry (MS) has become the major technology for elucidation of glycan structures.					
30999248	9	13	theme	tree	1270:1273	arg1	structure					1275:1283	the underlying glycan tree structure	1248:1283	the underlying glycan tree structure	1248:1283	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	15	14	theme	first	2244:2248	arg1	score					2264:2268	the manually-validated structure first three highest score	2211:2268	the manually-validated structure first three highest score	2211:2268	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	3	15	theme	structures	297:306	arg1	details					280:286	the fine details	271:286	the fine details of their structures	271:306	These roles are primarily determined by the fine details of their structures, making glycan structural identification highly desirable.					
30999248	13	16	theme	isomorphic	1811:1820	arg1	subtrees					1822:1829	isomorphic subtrees	1811:1829	isomorphic subtrees	1811:1829	Unlike the traditional enumerating followed by filtering strategy, our approach performed deisomorphism to remove isomorphic subtrees, and ruled out invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency.					
30999248	2	17	theme	biological	209:218	arg1	processes					220:228	biological processes	209:228	biological processes	209:228	Glycans play important roles in a great variety of biological processes.					
30999248	5	18	theme	mass	525:528	arg1	spectra					530:536	mass spectra	525:536	mass spectra	525:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	9	19	theme	corresponding	1130:1142	arg1	ion					1153:1155	its corresponding fragment ion	1126:1155	its corresponding fragment ion	1126:1155	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	3	20	theme	structural	323:332	arg1	identification					334:347	glycan structural identification	316:347	glycan structural identification highly desirable	316:364	These roles are primarily determined by the fine details of their structures, making glycan structural identification highly desirable.					
30999248	12	21	theme	complete	1605:1612	arg1	structures					1614:1623	the generated complete structures	1591:1623	the generated complete structures	1591:1623	Finally the generated complete structures were ranked according to their compatibility to the input mass spectra.					
30999248	16	22	theme	perfect	2421:2427	arg1	agreement					2429:2437	perfect agreement	2421:2437	perfect agreement with previous studies and CE analysis	2421:2475	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	0	23	theme	merging	70:76	arg1	strategy					78:85	tree merging strategy	65:85	tree merging strategy	65:85	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	4	24	theme	major	405:409	arg1	technology					411:420	the major technology	401:420	the major technology for elucidation of glycan structures	401:457	Mass spectrometry (MS) has become the major technology for elucidation of glycan structures.					
30999248	11	25	theme	complete	1544:1551	arg1	structure					1558:1566	a complete tree structure	1542:1566	a complete tree structure	1542:1566	During this process, the intermediate structures were represented as subtrees, which were merged iteratively until a complete tree structure was generated.					
30999248	9	26	theme	fragment	1144:1151	arg1	ion					1153:1155	its corresponding fragment ion	1126:1155	its corresponding fragment ion	1126:1155	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	1	27	theme	specific	132:139	arg1	structures					146:155	specific tree structures	132:155	specific tree structures	132:155	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	9	28	theme	monosaccharide	1096:1109	arg1	composition					1111:1121	monosaccharide composition	1096:1121	monosaccharide composition of its corresponding fragment ion	1096:1155	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	10	29	theme	connecting	1305:1314	arg1	constraints					1316:1326	these connecting constraints	1299:1326	these connecting constraints	1299:1326	According to these connecting constraints, we next merged constituting monosaccharides of the glycan into a complete structure step by step.					
30999248	0	30	theme	De	0:1	arg1	glycan					8:13	De novo glycan	0:13	De novo glycan	0:13	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	1	31	with	molecules	117:125	arg1	structures					146:155	specific tree structures	132:155	specific tree structures	132:155	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	5	32	theme	filtering	590:598	arg1	approaches					600:609	filtering approaches	590:609	filtering approaches	590:609	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	1	33	theme	MOTIVATION	88:97	arg1	Glycans					99:105	MOTIVATION Glycans	88:105	MOTIVATION Glycans	88:105	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	1	33	theme	MOTIVATION	88:97	arg1	molecules					117:125	large molecules	111:125	large molecules with specific tree structures	111:155	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	12	34	theme	mass	1683:1686	arg1	spectra					1688:1694	the input mass spectra	1673:1694	the input mass spectra	1673:1694	Finally the generated complete structures were ranked according to their compatibility to the input mass spectra.					
30999248	15	35	theme	test	2104:2107	arg1	standards					2128:2136	10 N-glycan standards	2116:2136	10 N-glycan standards	2116:2136	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	15	35	theme	test	2104:2107	arg1	set					2109:2111	a test set	2102:2111	a test set of 10 N-glycan standards	2102:2136	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	10	36	theme	constituting	1344:1355	arg1	monosaccharides					1357:1371	constituting monosaccharides	1344:1371	constituting monosaccharides of the glycan	1344:1385	According to these connecting constraints, we next merged constituting monosaccharides of the glycan into a complete structure step by step.					
30999248	13	37	theme	merging	1919:1925	arg1	step					1927:1930	each tree merging step	1909:1930	each tree merging step	1909:1930	Unlike the traditional enumerating followed by filtering strategy, our approach performed deisomorphism to remove isomorphic subtrees, and ruled out invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency.					
30999248	5	38	dep	enumerating	566:576	arg1	followed					578:585	followed	578:585	followed by filtering approaches, heuristic and dynamic programming-based approaches	578:661	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	9	39	theme	ion	1153:1155	arg1	composition					1111:1121	monosaccharide composition	1096:1121	monosaccharide composition of its corresponding fragment ion	1096:1155	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	15	40	theme	manually-validated	2215:2232	arg1	score					2264:2268	the manually-validated structure first three highest score	2211:2268	the manually-validated structure first three highest score	2211:2268	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	8	41	theme	efficient	943:951	arg1	approach					966:973	an efficient and reliable approach	940:973	an efficient and reliable approach to glycan structure identification using tree merging strategy	940:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	10	42	theme	glycan	1380:1385	arg1	monosaccharides					1357:1371	constituting monosaccharides	1344:1371	constituting monosaccharides of the glycan	1344:1385	According to these connecting constraints, we next merged constituting monosaccharides of the glycan into a complete structure step by step.					
30999248	0	43	theme	mass	46:49	arg1	spectra					51:57	mass spectra	46:57	mass spectra using tree merging strategy	46:85	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	4	44	theme	Mass	367:370	arg1	MS					386:387	MS	386:387	MS	386:387	Mass spectrometry (MS) has become the major technology for elucidation of glycan structures.					
30999248	4	44	theme	Mass	367:370	arg1	spectrometry					372:383	Mass spectrometry	367:383	Mass spectrometry (MS)	367:388	Mass spectrometry (MS) has become the major technology for elucidation of glycan structures.					
30999248	10	45	theme	structure	1403:1411	arg1	step					1413:1416	a complete structure step	1392:1416	a complete structure step	1392:1416	According to these connecting constraints, we next merged constituting monosaccharides of the glycan into a complete structure step by step.					
30999248	5	46	theme	Most	460:463	arg1	approaches					473:482	Most de novo approaches	460:482	Most de novo approaches to glycan structural identification from mass spectra	460:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	0	47	dep	De	0:1	arg1	novo					3:6	novo	3:6	novo	3:6	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	8	48	theme	structure	985:993	arg1	identification					995:1008	glycan structure identification	978:1008	glycan structure identification using tree merging strategy	978:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	13	49	theme	connection	1883:1892	arg1	constraints					1894:1904	the connection constraints	1879:1904	the connection constraints	1879:1904	Unlike the traditional enumerating followed by filtering strategy, our approach performed deisomorphism to remove isomorphic subtrees, and ruled out invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency.					
30999248	6	50	theme	low	692:694	arg1	efficiency					696:705	its low efficiency	688:705	its low efficiency	688:705	The former suffers from its low efficiency while the latter two suffer from the possibility of missing the actual glycan structures.					
30999248	6	51	theme	glycan	778:783	arg1	structures					785:794	the actual glycan structures	767:794	the actual glycan structures	767:794	The former suffers from its low efficiency while the latter two suffer from the possibility of missing the actual glycan structures.					
30999248	8	52	theme	merging	1021:1027	arg1	strategy					1029:1036	tree merging strategy	1016:1036	tree merging strategy	1016:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	16	53	theme	subsequent	2333:2342	arg1	assignment					2354:2363	subsequent structure assignment	2333:2363	subsequent structure assignment	2333:2363	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	5	54	theme	programming-based	634:650	arg1	approaches					652:661	dynamic programming-based approaches	626:661	dynamic programming-based approaches	626:661	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	16	55	theme	CE	2465:2466	arg1	analysis					2468:2475	CE analysis	2465:2475	CE analysis	2465:2475	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	5	56	theme	structural	494:503	arg1	identification					505:518	glycan structural identification	487:518	glycan structural identification from mass spectra	487:536	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	14	57	theme	complete	1992:1999	arg1	structures					2001:2010	all complete structures	1988:2010	all complete structures satisfying the connection constraints	1988:2048	In addition, all complete structures satisfying the connection constraints were enumerated without any missing structure.					
30999248	16	58	gly	glycoprotein	2390:2401	arg1	glycoprotein					2390:2401	glycoprotein mAb	2390:2405	glycoprotein mAb	2390:2405	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	8	59	dep	RESULTS	907:913	arg1	propose					932:938	propose	932:938	propose an efficient and reliable approach to glycan structure identification using tree merging strategy	932:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	8	60	theme	reliable	957:964	arg1	approach					966:973	an efficient and reliable approach	940:973	an efficient and reliable approach to glycan structure identification using tree merging strategy	940:1036	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	4	61	theme	structures	448:457	arg1	elucidation					426:436	elucidation	426:436	elucidation of glycan structures	426:457	Mass spectrometry (MS) has become the major technology for elucidation of glycan structures.					
30999248	16	62	theme	glycoprotein	2390:2401	arg1	mAb					2403:2405	glycoprotein mAb	2390:2405	glycoprotein mAb	2390:2405	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	9	63	theme	glycan	1263:1268	arg1	structure					1275:1283	the underlying glycan tree structure	1248:1283	the underlying glycan tree structure	1248:1283	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	7	64	theme	glycan	844:849	arg1	structures					851:860	glycan structures	844:860	glycan structures from mass spectra	844:878	Thus, how to reliably and efficiently identify glycan structures from mass spectra still remains challenging.					
30999248	15	65	theme	structure	2234:2242	arg1	score					2264:2268	the manually-validated structure first three highest score	2211:2268	the manually-validated structure first three highest score	2211:2268	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	8	66	from	RESULTS	907:913	arg1	study					923:927	this study	918:927	this study	918:927	RESULTS In this study we propose an efficient and reliable approach to glycan structure identification using tree merging strategy.					
30999248	16	67	attach	released	2376:2383	arg2	glycans					2368:2374	glycans	2368:2374	glycans released from glycoprotein mAb	2368:2405	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	16	67	attach	released	2376:2383	arg1	mAb					2403:2405	glycoprotein mAb	2390:2405	glycoprotein mAb	2390:2405	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	2	68	theme	processes	220:228	arg1	variety					198:204	a great variety	190:204	a great variety of biological processes	190:228	Glycans play important roles in a great variety of biological processes.					
30999248	2	68	theme	processes	220:228	arg1	processes					220:228	biological processes	209:228	biological processes	209:228	Glycans play important roles in a great variety of biological processes.					
30999248	1	69	theme	large	111:115	arg1	Glycans					99:105	MOTIVATION Glycans	88:105	MOTIVATION Glycans	88:105	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	1	69	theme	large	111:115	arg1	molecules					117:125	large molecules	111:125	large molecules with specific tree structures	111:155	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	12	70	theme	generated	1595:1603	arg1	structures					1614:1623	the generated complete structures	1591:1623	the generated complete structures	1591:1623	Finally the generated complete structures were ranked according to their compatibility to the input mass spectra.					
30999248	9	71	theme	MS	1057:1058	arg1	peak					1060:1063	each MS peak	1052:1063	each MS peak	1052:1063	Briefly, for each MS peak, our approach first calculated monosaccharide composition of its corresponding fragment ion, and then built a constraint that forces these monosaccharides to be directly connected in the underlying glycan tree structure.					
30999248	0	72	theme	tree	65:68	arg1	strategy					78:85	tree merging strategy	65:85	tree merging strategy	65:85	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	1	73	theme	tree	141:144	arg1	structures					146:155	specific tree structures	132:155	specific tree structures	132:155	MOTIVATION Glycans are large molecules with specific tree structures.					
30999248	5	74	dep	de	465:466	arg1	novo					468:471	novo	468:471	novo	468:471	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	11	75	theme	tree	1553:1556	arg1	structure					1558:1566	a complete tree structure	1542:1566	a complete tree structure	1542:1566	During this process, the intermediate structures were represented as subtrees, which were merged iteratively until a complete tree structure was generated.					
30999248	3	76	theme	glycan	316:321	arg1	identification					334:347	glycan structural identification	316:347	glycan structural identification highly desirable	316:364	These roles are primarily determined by the fine details of their structures, making glycan structural identification highly desirable.					
30999248	16	77	theme	previous	2444:2451	arg1	studies					2453:2459	previous studies	2444:2459	previous studies	2444:2459	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	0	78	from	spectra	51:57	arg1	identification					26:39	identification	26:39	identification from mass spectra using tree merging strategy	26:85	De novo glycan structural identification from mass spectra using tree merging strategy.					
30999248	5	79	theme	dynamic	626:632	arg1	approaches					652:661	dynamic programming-based approaches	626:661	dynamic programming-based approaches	626:661	Most de novo approaches to glycan structural identification from mass spectra fall into three categories: enumerating followed by filtering approaches, heuristic and dynamic programming-based approaches.					
30999248	3	80	theme	desirable	356:364	arg1	identification					334:347	glycan structural identification	316:347	glycan structural identification highly desirable	316:364	These roles are primarily determined by the fine details of their structures, making glycan structural identification highly desirable.					
30999248	11	81	theme	intermediate	1452:1463	arg1	subtrees					1496:1503	subtrees	1496:1503	subtrees	1496:1503	During this process, the intermediate structures were represented as subtrees, which were merged iteratively until a complete tree structure was generated.					
30999248	11	81	theme	intermediate	1452:1463	arg1	structures					1465:1474	the intermediate structures	1448:1474	the intermediate structures	1448:1474	During this process, the intermediate structures were represented as subtrees, which were merged iteratively until a complete tree structure was generated.					
30999248	15	82	theme	N-glycan	2119:2126	arg1	standards					2128:2136	10 N-glycan standards	2116:2136	10 N-glycan standards	2116:2136	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	14	83	theme	missing	2078:2084	arg1	structure					2086:2094	any missing structure	2074:2094	any missing structure	2074:2094	In addition, all complete structures satisfying the connection constraints were enumerated without any missing structure.					
30999248	12	84	theme	input	1677:1681	arg1	spectra					1688:1694	the input mass spectra	1673:1694	the input mass spectra	1673:1694	Finally the generated complete structures were ranked according to their compatibility to the input mass spectra.					
30999248	13	85	theme	tree	1914:1917	arg1	step					1927:1930	each tree merging step	1909:1930	each tree merging step	1909:1930	Unlike the traditional enumerating followed by filtering strategy, our approach performed deisomorphism to remove isomorphic subtrees, and ruled out invalid structures that violates the connection constraints at each tree merging step, thus significantly increasing efficiency.					
30999248	16	86	theme	structure	2344:2352	arg1	assignment					2354:2363	subsequent structure assignment	2333:2363	subsequent structure assignment	2333:2363	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	2	87	theme	important	171:179	arg1	roles					181:185	important roles	171:185	important roles	171:185	Glycans play important roles in a great variety of biological processes.					
30999248	6	88	theme	actual	771:776	arg1	structures					785:794	the actual glycan structures	767:794	the actual glycan structures	767:794	The former suffers from its low efficiency while the latter two suffer from the possibility of missing the actual glycan structures.					
30999248	16	89	with	agreement	2429:2437	arg1	studies					2453:2459	previous studies	2444:2459	previous studies	2444:2459	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	16	89	with	agreement	2429:2437	arg1	analysis					2468:2475	CE analysis	2465:2475	CE analysis	2465:2475	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	14	90	theme	connection	2027:2036	arg1	constraints					2038:2048	the connection constraints	2023:2048	the connection constraints	2023:2048	In addition, all complete structures satisfying the connection constraints were enumerated without any missing structure.					
30999248	2	91	theme	great	192:196	arg1	variety					198:204	a great variety	190:204	a great variety of biological processes	190:228	Glycans play important roles in a great variety of biological processes.					
30999248	2	91	theme	great	192:196	arg1	processes					220:228	biological processes	209:228	biological processes	209:228	Glycans play important roles in a great variety of biological processes.					
30999248	7	92	from	spectra	872:878	arg1	structures					851:860	glycan structures	844:860	glycan structures from mass spectra	844:878	Thus, how to reliably and efficiently identify glycan structures from mass spectra still remains challenging.					
30999248	16	93	theme	glycans	2368:2374	arg1	profiling					2319:2327	profiling	2319:2327	profiling	2319:2327	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	16	93	theme	glycans	2368:2374	arg1	assignment					2354:2363	subsequent structure assignment	2333:2363	subsequent structure assignment	2333:2363	We further successfully applied our approach to profiling and subsequent structure assignment of glycans released from glycoprotein mAb, which was in perfect agreement with previous studies and CE analysis.					
30999248	15	94	theme	structural	2165:2174	arg1	identification					2176:2189	structural identification	2165:2189	structural identification in minutes	2165:2200	Over a test set of 10 N-glycan standards, our approach accomplished structural identification in minutes and gave the manually-validated structure first three highest score.					
30999248	10	95	theme	complete	1394:1401	arg1	step					1413:1416	a complete structure step	1392:1416	a complete structure step	1392:1416	According to these connecting constraints, we next merged constituting monosaccharides of the glycan into a complete structure step by step.					
29730764	1	0	theme	O-glycopeptides	144:158	arg1	mixture					112:118	A very complex mixture	97:118	A very complex mixture	97:118	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	4	1	theme	success	712:718	arg1	rate					720:723	the identification success rate	693:723	the identification success rate	693:723	Thus, we investigated the reasons why was it so, to help to improve the identification success rate.					
29730764	8	2	theme	ETD	1350:1352	arg1	factor					1385:1390	a limiting factor	1374:1390	a limiting factor	1374:1390	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	8	2	theme	ETD	1350:1352	arg1	efficiency					1354:1363	ETD efficiency	1350:1363	ETD efficiency	1350:1363	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	0	3	theme	Mixtures	87:94	arg1	Characterization					37:52	the High-Throughput Characterization	17:52	the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures	17:94	Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.					
29730764	6	4	dep	provide	921:927	arg1	so					980:981	so	980:981	so	980:981	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	9	5	theme	results	1531:1537	arg1	limited					1512:1518	limited	1512:1518	limited	1512:1518	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	2	6	gly	glycopeptides	486:498	arg2	glycopeptides					486:498	O-linked glycopeptides	477:498	O-linked glycopeptides	477:498	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	1	7	theme	two-step	246:253	arg1	enrichment					271:280	a two-step lectin affinity enrichment	244:280	a two-step lectin affinity enrichment	244:280	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	1	8	theme	glycopeptide	341:352	arg1	spectra					360:366	approximately 45,000 glycopeptide EThcD spectra	320:366	approximately 45,000 glycopeptide EThcD spectra	320:366	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	6	9	theme	tetra-	1084:1089	arg1	presence					1070:1077	the presence	1066:1077	the presence of a tetra- or hexasaccharide structure	1066:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	1	10	theme	lectin	255:260	arg1	enrichment					271:280	a two-step lectin affinity enrichment	244:280	a two-step lectin affinity enrichment	244:280	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	2	11	theme	O-linked	477:484	arg1	glycopeptides					486:498	O-linked glycopeptides	477:498	O-linked glycopeptides	477:498	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	1	12	theme	EThcD	354:358	arg1	spectra					360:366	approximately 45,000 glycopeptide EThcD spectra	320:366	approximately 45,000 glycopeptide EThcD spectra	320:366	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	9	13	theme	search	1560:1565	arg1	engines					1567:1573	the two search engines	1552:1573	the two search engines	1552:1573	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	3	14	from	searches	582:589	arg1	low					571:573	low	571:573	low	571:573	The identification rate was very low in all searches, even when results were combined.					
29730764	3	14	from	searches	582:589	arg1	rate					557:560	The identification rate	538:560	The identification rate	538:560	The identification rate was very low in all searches, even when results were combined.					
29730764	5	15	theme	larger	788:793	arg1	ions					810:813	larger glycan oxonium ions	788:813	larger glycan oxonium ions	788:813	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	6	16	theme	Y	913:913	arg1	ions					915:918	terminal Y ions	904:918	terminal Y ions	904:918	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	2	17	used	used	425:428	arg2	Prospector					408:417	Protein Prospector	400:417	Protein Prospector	400:417	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	2	17	used	used	425:428	arg2	Byonic					389:394	Byonic	389:394	Byonic	389:394	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	2	17	used	used	425:428	arg2	engines					380:386	Two search engines	369:386	Two search engines	369:386	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	1	18	dep	intact	123:128	arg1	human					131:135	human	131:135	human	131:135	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	5	19	theme	glycan	795:800	arg1	ions					810:813	larger glycan oxonium ions	788:813	larger glycan oxonium ions	788:813	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	2	20	theme	data	460:463	arg1	interpretation					438:451	the interpretation	434:451	the interpretation of the data	434:463	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	6	21	theme	such	1165:1168	arg1	glycans					1170:1176	such glycans	1165:1176	such glycans	1165:1176	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	6	22	theme	terminal	904:911	arg1	ions					915:918	terminal Y ions	904:918	terminal Y ions	904:918	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	3	23	theme	identification	542:555	arg1	low					571:573	low	571:573	low	571:573	The identification rate was very low in all searches, even when results were combined.					
29730764	3	23	theme	identification	542:555	arg1	rate					557:560	The identification rate	538:560	The identification rate	538:560	The identification rate was very low in all searches, even when results were combined.					
29730764	2	24	theme	search	373:378	arg1	engines					380:386	Two search engines	369:386	Two search engines	369:386	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	2	24	theme	search	373:378	arg1	Byonic					389:394	Byonic	389:394	Byonic	389:394	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	2	24	theme	search	373:378	arg1	Prospector					408:417	Protein Prospector	400:417	Protein Prospector	400:417	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	8	25	theme	glycosylated	1408:1419	arg1	peptides					1421:1428	highly glycosylated peptides	1401:1428	highly glycosylated peptides	1401:1428	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	1	26	theme	affinity	262:269	arg1	enrichment					271:280	a two-step lectin affinity enrichment	244:280	a two-step lectin affinity enrichment	244:280	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	9	27	theme	O-linked	1641:1648	arg1	glycopeptides					1650:1662	O-linked glycopeptides	1641:1662	O-linked glycopeptides	1641:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	6	28	contain	containing	1154:1163	arg2	glycans					1170:1176	such glycans	1165:1176	such glycans	1165:1176	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	6	28	contain	containing	1154:1163	arg1	molecules					1144:1152	all molecules	1140:1152	all molecules containing such glycans	1140:1176	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	1	29	theme	tryptic	179:185	arg1	digest					187:192	the tryptic digest	175:192	the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment	175:280	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	6	30	dep	tetra-	1084:1089	arg1	structure					1109:1117	structure	1109:1117	structure	1109:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	1	31	theme	complex	104:110	arg1	mixture					112:118	A very complex mixture	97:118	A very complex mixture	97:118	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	1	32	gly	glycopeptide	341:352	arg2	glycopeptide					341:352	glycopeptide	341:352	glycopeptide	341:352	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	9	33	dep	limited	1512:1518	arg1	overlap					1520:1526	overlap	1520:1526	overlap	1520:1526	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	2	34	theme	separate	519:526	arg1	searches					528:535	separate searches	519:535	separate searches	519:535	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	6	35	theme	important	929:937	arg1	information					939:949	important information	929:949	important information about the glycan(s) present	929:977	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	7	36	theme	oxonium	1310:1316	arg1	ions					1318:1321	these oxonium ions	1304:1321	these oxonium ions	1304:1321	Our study showed that intact glycans frequently do not survive even mild supplemental activation, meaning one cannot rely on these oxonium ions exclusively.					
29730764	3	37	from	low	571:573	arg1	searches					582:589	all searches	578:589	all searches	578:589	The identification rate was very low in all searches, even when results were combined.					
29730764	2	38	theme	Protein	400:406	arg1	engines					380:386	Two search engines	369:386	Two search engines	369:386	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	2	38	theme	Protein	400:406	arg1	Prospector					408:417	Protein Prospector	400:417	Protein Prospector	400:417	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	6	39	theme	glycan	1035:1040	arg1	ions					1050:1053	glycan oxonium ions	1035:1053	glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure	1035:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	7	40	theme	intact	1201:1206	arg1	glycans					1208:1214	intact glycans	1201:1214	intact glycans	1201:1214	Our study showed that intact glycans frequently do not survive even mild supplemental activation, meaning one cannot rely on these oxonium ions exclusively.					
29730764	6	41	theme	present	971:977	arg1	s					968:968	the glycan(s)	957:969	the glycan(s) present	957:977	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	1	42	theme	urinary	197:203	arg1	proteins					205:212	urinary proteins	197:212	urinary proteins of three healthy donors	197:236	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	0	43	theme	High-Throughput	21:35	arg1	Characterization					37:52	the High-Throughput Characterization	17:52	the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures	17:94	Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.					
29730764	5	44	theme	oxonium	802:808	arg1	ions					810:813	larger glycan oxonium ions	788:813	larger glycan oxonium ions	788:813	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	5	45	link	O-linked	738:745	arg1	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	9	46	gly	glycopeptides	1650:1662	arg2	glycopeptides					1650:1662	O-linked glycopeptides	1641:1662	O-linked glycopeptides	1641:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	1	47	theme	proteins	205:212	arg1	digest					187:192	the tryptic digest	175:192	the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment	175:280	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	0	48	theme	Complex	57:63	arg1	Mixtures					87:94	Complex Intact O-Glycopeptide Mixtures	57:94	Complex Intact O-Glycopeptide Mixtures	57:94	Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.					
29730764	9	49	theme	automated	1608:1616	arg1	interpretation					1623:1636	automated data interpretation	1608:1636	automated data interpretation of O-linked glycopeptides	1608:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	9	50	theme	glycopeptides	1650:1662	arg1	interpretation					1623:1636	automated data interpretation	1608:1636	automated data interpretation of O-linked glycopeptides	1608:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	10	51	theme	Graphical	1699:1707	arg1	abstract					1709:1716	Graphical abstract	1699:1716	Graphical abstract	1699:1716	Graphical abstract ᅟ.					
29730764	8	52	theme	only	1435:1438	arg1	related					1474:1480	related	1474:1480	related	1474:1480	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	8	52	theme	only	1435:1438	arg1	information					1440:1450	the only information	1431:1450	the only information revealed in EThcD	1431:1468	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	6	53	theme	oxonium	1042:1048	arg1	ions					1050:1053	glycan oxonium ions	1035:1053	glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure	1035:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	5	54	theme	O-linked	738:745	arg1	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	9	55	theme	data	1618:1621	arg1	interpretation					1623:1636	automated data interpretation	1608:1636	automated data interpretation of O-linked glycopeptides	1608:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	8	56	theme	limiting	1376:1383	arg1	factor					1385:1390	a limiting factor	1374:1390	a limiting factor	1374:1390	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	8	56	theme	limiting	1376:1383	arg1	efficiency					1354:1363	ETD efficiency	1350:1363	ETD efficiency	1350:1363	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	1	57	theme	intact	123:128	arg1	N-					137:138	intact, human N-	123:138	intact, human N-	123:138	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	7	58	theme	activation	1265:1274	arg1	meaning					1277:1283	mild supplemental activation, meaning one	1247:1287	meaning	1277:1283	Our study showed that intact glycans frequently do not survive even mild supplemental activation, meaning one cannot rely on these oxonium ions exclusively.					
29730764	5	59	gly	glycopeptides	747:759	arg2	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	Focusing on O-linked glycopeptides, we noticed that in EThcD, larger glycan oxonium ions better survive the activation than those in HCD.					
29730764	9	60	link	O-linked	1641:1648	arg1	glycopeptides					1650:1662	O-linked glycopeptides	1641:1662	O-linked glycopeptides	1641:1662	The limited overlap of results delivered by the two search engines draws attention to the fact that automated data interpretation of O-linked glycopeptides is not even close to being solved.					
29730764	1	61	gly	O-glycopeptides	144:158	arg2	O-glycopeptides					144:158	O-glycopeptides	144:158	O-glycopeptides	144:158	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	4	62	theme	identification	697:710	arg1	rate					720:723	the identification success rate	693:723	the identification success rate	693:723	Thus, we investigated the reasons why was it so, to help to improve the identification success rate.					
29730764	2	63	link	O-linked	477:484	arg1	glycopeptides					486:498	O-linked glycopeptides	477:498	O-linked glycopeptides	477:498	Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches.					
29730764	8	64	gly	glycosylated	1408:1419	arg1	peptides					1421:1428	highly glycosylated peptides	1401:1428	highly glycosylated peptides	1401:1428	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	8	65	theme	glycan	1489:1494	arg1	structures					1496:1505	the glycan structures	1485:1505	the glycan structures	1485:1505	We found that ETD efficiency is still a limiting factor, and for highly glycosylated peptides, the only information revealed in EThcD was related to the glycan structures.					
29730764	0	66	theme	O-Glycopeptide	72:85	arg1	Mixtures					87:94	Complex Intact O-Glycopeptide Mixtures	57:94	Complex Intact O-Glycopeptide Mixtures	57:94	Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.					
29730764	6	67	theme	glycan	961:966	arg1	s					968:968	the glycan(s)	957:969	the glycan(s) present	957:977	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	7	68	theme	supplemental	1252:1263	arg1	meaning					1277:1283	mild supplemental activation, meaning one	1247:1287	meaning	1277:1283	Our study showed that intact glycans frequently do not survive even mild supplemental activation, meaning one cannot rely on these oxonium ions exclusively.					
29730764	1	69	theme	N-	137:138	arg1	mixture					112:118	A very complex mixture	97:118	A very complex mixture	97:118	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	7	70	theme	mild	1247:1250	arg1	meaning					1277:1283	mild supplemental activation, meaning one	1247:1287	meaning	1277:1283	Our study showed that intact glycans frequently do not survive even mild supplemental activation, meaning one cannot rely on these oxonium ions exclusively.					
29730764	1	71	theme	healthy	223:229	arg1	donors					231:236	three healthy donors	217:236	three healthy donors	217:236	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
29730764	0	72	theme	Intact	65:70	arg1	Mixtures					87:94	Complex Intact O-Glycopeptide Mixtures	57:94	Complex Intact O-Glycopeptide Mixtures	57:94	Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.					
29730764	6	73	theme	hexasaccharide	1094:1107	arg1	presence					1070:1077	the presence	1066:1077	the presence of a tetra- or hexasaccharide structure	1066:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	6	74	theme	filtering	1007:1015	arg1	peaklists					1021:1029	the peaklists	1017:1029	filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure	1007:1117	These fragments, combined with reducing terminal Y ions, provide important information about the glycan(s) present, so we investigated whether filtering the peaklists for glycan oxonium ions indicating the presence of a tetra- or hexasaccharide structure would help to reveal all molecules containing such glycans.					
29730764	1	75	theme	donors	231:236	arg1	proteins					205:212	urinary proteins	197:212	urinary proteins of three healthy donors	197:236	A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra.					
31500407	2	0	theme	membrane	356:363	arg1	anchors					365:371	membrane anchors	356:371	membrane anchors	356:371	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	1	theme	designed	475:482	arg1	domains					484:490	newly designed domains	469:490	newly designed domains	469:490	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	3	2	theme	additional	635:644	arg1	permutations					646:657	additional permutations	635:657	additional permutations	635:657	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	5	3	theme	glycan	875:880	arg1	structures					882:891	glycan structures	875:891	glycan structures	875:891	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	1	4	theme	eukaryotic	176:185	arg1	cells					187:191	eukaryotic cells	176:191	eukaryotic cells	176:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	5	5	theme	peptide	919:925	arg1	sequence					936:943	the underlying peptide backbone sequence	904:943	the underlying peptide backbone sequence	904:943	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	4	6	theme	mucins	783:788	arg1	engineering					768:778	engineering	768:778	engineering of mucins	768:788	We apply the library to explore sequence-specific effects on glycosylation for engineering of mucins.					
31500407	4	7	theme	sequence-specific	721:737	arg1	effects					739:745	sequence-specific effects	721:745	sequence-specific effects on glycosylation for engineering of mucins	721:788	We apply the library to explore sequence-specific effects on glycosylation for engineering of mucins.					
31500407	0	8	theme	Sequence-Specific	0:16	arg1	Mucins					18:23	Sequence-Specific Mucins	0:23	Sequence-Specific Mucins for Glycocalyx Engineering	0:50	Sequence-Specific Mucins for Glycocalyx Engineering.					
31500407	1	9	from	editing	165:171	arg1	cells					187:191	eukaryotic cells	176:191	eukaryotic cells	176:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	1	10	theme	stable	82:87	arg1	synthesis					106:114	the stable and controllable synthesis	78:114	the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells	78:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	2	11	theme	native	444:449	arg1	repeats					457:463	codon-optimized native mucin repeats	428:463	codon-optimized native mucin repeats	428:463	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	6	12	from	motifs	1003:1008	arg1	tail					1035:1038	the mucin cytoplasmic tail	1013:1038	the mucin cytoplasmic tail	1013:1038	Glycosylation could also be influenced through recycling motifs on the mucin cytoplasmic tail.					
31500407	4	13	from	effects	739:745	arg1	glycosylation					750:762	glycosylation	750:762	glycosylation	750:762	We apply the library to explore sequence-specific effects on glycosylation for engineering of mucins.					
31500407	2	14	theme	bricks	326:331	arg1	library					301:307	a library	299:307	a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins	299:511	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	15	theme	codon-optimized	428:442	arg1	repeats					457:463	codon-optimized native mucin repeats	428:463	codon-optimized native mucin repeats	428:463	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	16	theme	DNA	322:324	arg1	bricks					326:331	swappable DNA bricks	312:331	swappable DNA bricks	312:331	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	17	theme	cytoplasmic	374:384	arg1	motifs					386:391	cytoplasmic motifs	374:391	cytoplasmic motifs	374:391	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	0	18	theme	Glycocalyx	29:38	arg1	Engineering					40:50	Glycocalyx Engineering	29:50	Glycocalyx Engineering	29:50	Sequence-Specific Mucins for Glycocalyx Engineering.					
31500407	2	19	theme	cDNA	271:274	arg1	construction					276:287	cDNA construction	271:287	cDNA construction	271:287	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	7	20	theme	mucin	1060:1064	arg1	parts					1066:1070	the mucin parts inventory	1056:1080	the mucin parts inventory presented here	1056:1095	We expect that the mucin parts inventory presented here can be broadly applied for glycocalyx research and mucin-based biotechnologies.					
31500407	5	21	dep	Core	857:860	arg1	structures					882:891	glycan structures	875:891	glycan structures	875:891	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	2	22	theme	swappable	312:320	arg1	bricks					326:331	swappable DNA bricks	312:331	swappable DNA bricks	312:331	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	1	23	theme	controllable	93:104	arg1	synthesis					106:114	the stable and controllable synthesis	78:114	the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells	78:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	5	24	theme	backbone	927:934	arg1	sequence					936:943	the underlying peptide backbone sequence	904:943	the underlying peptide backbone sequence	904:943	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	3	25	theme	mucins	548:553	arg1	library					529:535	a library	527:535	a library of over 50 mucins, each with unique chemical, structural, and optical properties	527:616	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	5	26	theme	immature	825:832	arg1	Tn-antigen					843:852	the immature α-GalNAc Tn-antigen	821:852	the immature α-GalNAc Tn-antigen	821:852	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	2	27	theme	leader	343:348	arg1	tags					350:353	mucin leader tags	337:353	mucin leader tags	337:353	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	3	28	theme	chemical	573:580	arg1	properties					607:616	unique chemical, structural, and optical properties	566:616	unique chemical, structural, and optical properties	566:616	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	5	29	theme	α-GalNAc	834:841	arg1	Tn-antigen					843:852	the immature α-GalNAc Tn-antigen	821:852	the immature α-GalNAc Tn-antigen	821:852	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	7	30	theme	mucin-based	1148:1158	arg1	biotechnologies					1160:1174	mucin-based biotechnologies	1148:1174	mucin-based biotechnologies	1148:1174	We expect that the mucin parts inventory presented here can be broadly applied for glycocalyx research and mucin-based biotechnologies.					
31500407	1	31	gly	glycoproteins	136:148	arg1	glycoproteins					136:148	customized mucin glycoproteins	119:148	customized mucin glycoproteins	119:148	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	6	32	theme	recycling	993:1001	arg1	motifs					1003:1008	recycling motifs	993:1008	recycling motifs on the mucin cytoplasmic tail	993:1038	Glycosylation could also be influenced through recycling motifs on the mucin cytoplasmic tail.					
31500407	2	33	theme	biology-by-parts	242:257	arg1	approach					259:266	a biology-by-parts approach	240:266	a biology-by-parts approach to cDNA construction	240:287	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	34	theme	optical	398:404	arg1	reporters					406:414	optical reporters	398:414	optical reporters	398:414	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	7	35	theme	glycocalyx	1124:1133	arg1	research					1135:1142	glycocalyx research	1124:1142	glycocalyx research	1124:1142	We expect that the mucin parts inventory presented here can be broadly applied for glycocalyx research and mucin-based biotechnologies.					
31500407	7	36	theme	inventory	1072:1080	arg1	parts					1066:1070	the mucin parts inventory	1056:1080	the mucin parts inventory presented here	1056:1095	We expect that the mucin parts inventory presented here can be broadly applied for glycocalyx research and mucin-based biotechnologies.					
31500407	1	37	theme	customized	119:128	arg1	glycoproteins					136:148	customized mucin glycoproteins	119:148	customized mucin glycoproteins	119:148	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	2	38	theme	mucin	451:455	arg1	repeats					457:463	codon-optimized native mucin repeats	428:463	codon-optimized native mucin repeats	428:463	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	39	theme	synthesis	226:234	arg1	advantage					201:209	advantage	201:209	advantage of custom gene synthesis	201:234	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	39	theme	synthesis	226:234	arg1	approach					259:266	a biology-by-parts approach	240:266	a biology-by-parts approach to cDNA construction	240:287	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	3	40	theme	unique	566:571	arg1	properties					607:616	unique chemical, structural, and optical properties	566:616	unique chemical, structural, and optical properties	566:616	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	1	41	theme	mucin	130:134	arg1	glycoproteins					136:148	customized mucin glycoproteins	119:148	customized mucin glycoproteins	119:148	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	2	42	theme	gene	221:224	arg1	synthesis					226:234	custom gene synthesis	214:234	custom gene synthesis	214:234	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	6	43	theme	mucin	1017:1021	arg1	tail					1035:1038	the mucin cytoplasmic tail	1013:1038	the mucin cytoplasmic tail	1013:1038	Glycosylation could also be influenced through recycling motifs on the mucin cytoplasmic tail.					
31500407	1	44	theme	glycoproteins	136:148	arg1	synthesis					106:114	the stable and controllable synthesis	78:114	the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells	78:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	3	45	theme	structural	583:592	arg1	properties					607:616	unique chemical, structural, and optical properties	566:616	unique chemical, structural, and optical properties	566:616	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	6	46	theme	cytoplasmic	1023:1033	arg1	tail					1035:1038	the mucin cytoplasmic tail	1013:1038	the mucin cytoplasmic tail	1013:1038	Glycosylation could also be influenced through recycling motifs on the mucin cytoplasmic tail.					
31500407	2	47	theme	custom	214:219	arg1	synthesis					226:234	custom gene synthesis	214:234	custom gene synthesis	214:234	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	3	48	theme	optical	599:605	arg1	properties					607:616	unique chemical, structural, and optical properties	566:616	unique chemical, structural, and optical properties	566:616	We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed.					
31500407	5	49	theme	underlying	908:917	arg1	sequence					936:943	the underlying peptide backbone sequence	904:943	the underlying peptide backbone sequence	904:943	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	2	50	theme	mucin	337:341	arg1	tags					350:353	mucin leader tags	337:353	mucin leader tags	337:353	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	2	51	theme	synthetic	496:504	arg1	mucins					506:511	synthetic mucins	496:511	synthetic mucins	496:511	Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins.					
31500407	5	52	theme	Tn-antigen	843:852	arg1	extension					808:816	the extension	804:816	the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures	804:891	We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence.					
31500407	1	53	theme	Few	53:55	arg1	approaches					57:66	Few approaches	53:66	Few approaches	53:66	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
31500407	1	54	theme	glycocalyx	154:163	arg1	editing					165:171	glycocalyx editing	154:171	glycocalyx editing in eukaryotic cells	154:191	Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells.					
29374210	3	0	theme	ionization	518:527	arg1	condition					529:537	the electrospray ionization condition	501:537	the electrospray ionization condition using molecular dynamics simulations with enhanced sampling	501:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	4	1	theme	chemical	721:728	arg1	compositions					730:741	chemical compositions	721:741	chemical compositions	721:741	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	0	2	from	Characterization	0:15	arg1	Gas-Phase					76:84	the Gas-Phase	72:84	the Gas-Phase	72:84	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	2	3	with	isomers	306:312	arg1	differences					341:351	subtle conformational differences	319:351	subtle conformational differences	319:351	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	3	4	theme	enhanced	581:588	arg1	sampling					590:597	enhanced sampling	581:597	enhanced sampling	581:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	5	5	theme	physicochemical	779:793	arg1	basis					795:799	a physicochemical basis	777:799	a physicochemical basis of CCS for N-glycans	777:820	Providing a physicochemical basis of CCS for N-glycans helps not only to interpret IM-MS measurements but also to estimate CCSs of complex glycans.					
29374210	4	6	theme	folding	661:667	arg1	features					669:676	folding features	661:676	folding features	661:676	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	3	7	theme	dynamics	555:562	arg1	simulations					564:574	molecular dynamics simulations	545:574	molecular dynamics simulations with enhanced sampling	545:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	1	8	theme	structural	166:175	arg1	changes					177:183	structural changes	166:183	structural changes of biomolecules based on their collision cross section (CCS)	166:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	9	theme	Ion	87:89	arg1	IM-MS					119:123	IM-MS	119:123	IM-MS	119:123	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	9	theme	Ion	87:89	arg1	spectrometry					105:116	Ion mobility mass spectrometry	87:116	Ion mobility mass spectrometry (IM-MS)	87:124	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	9	theme	Ion	87:89	arg1	technique					131:139	a technique	129:139	a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS)	129:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	5	10	theme	complex	898:904	arg1	glycans					906:912	complex glycans	898:912	complex glycans	898:912	Providing a physicochemical basis of CCS for N-glycans helps not only to interpret IM-MS measurements but also to estimate CCSs of complex glycans.					
29374210	1	11	theme	mobility	91:98	arg1	IM-MS					119:123	IM-MS	119:123	IM-MS	119:123	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	11	theme	mobility	91:98	arg1	spectrometry					105:116	Ion mobility mass spectrometry	87:116	Ion mobility mass spectrometry (IM-MS)	87:124	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	11	theme	mobility	91:98	arg1	technique					131:139	a technique	129:139	a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS)	129:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	5	12	theme	glycans	906:912	arg1	CCSs					890:893	CCSs	890:893	CCSs of complex glycans	890:912	Providing a physicochemical basis of CCS for N-glycans helps not only to interpret IM-MS measurements but also to estimate CCSs of complex glycans.					
29374210	5	13	theme	IM-MS	850:854	arg1	measurements					856:867	IM-MS measurements	850:867	IM-MS measurements	850:867	Providing a physicochemical basis of CCS for N-glycans helps not only to interpret IM-MS measurements but also to estimate CCSs of complex glycans.					
29374210	2	14	theme	carbohydrate	293:304	arg1	isomers					306:312	separate carbohydrate isomers	284:312	separate carbohydrate isomers with subtle conformational differences	284:351	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	1	15	theme	biomolecules	188:199	arg1	changes					177:183	structural changes	166:183	structural changes of biomolecules based on their collision cross section (CCS)	166:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	0	16	theme	Ensembles	35:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.	0:85	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	2	17	theme	separate	284:291	arg1	isomers					306:312	separate carbohydrate isomers	284:312	separate carbohydrate isomers with subtle conformational differences	284:351	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	1	18	theme	mass	100:103	arg1	IM-MS					119:123	IM-MS	119:123	IM-MS	119:123	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	18	theme	mass	100:103	arg1	spectrometry					105:116	Ion mobility mass spectrometry	87:116	Ion mobility mass spectrometry (IM-MS)	87:124	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	18	theme	mass	100:103	arg1	technique					131:139	a technique	129:139	a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS)	129:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	2	19	from	advances	254:261	arg1	IM-MS					266:270	IM-MS	266:270	IM-MS	266:270	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	3	20	theme	gas-phase	475:483	arg1	N-glycans					485:493	gas-phase N-glycans	475:493	gas-phase N-glycans	475:493	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	0	21	theme	Protonated	48:57	arg1	N-glycans					59:67	Protonated N-glycans	48:67	Protonated N-glycans	48:67	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	3	22	theme	electrospray	505:516	arg1	condition					529:537	the electrospray ionization condition	501:537	the electrospray ionization condition using molecular dynamics simulations with enhanced sampling	501:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	4	23	theme	N-glycans	681:689	arg1	features					669:676	folding features	661:676	folding features	661:676	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	3	24	theme	conformational	447:460	arg1	ensembles					462:470	conformational ensembles	447:470	conformational ensembles of gas-phase N-glycans	447:493	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	2	25	theme	atomic	391:396	arg1	structure					398:406	atomic structure	391:406	atomic structure	391:406	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	3	26	with	simulations	564:574	arg1	sampling					590:597	enhanced sampling	581:597	enhanced sampling	581:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	0	27	from	Gas-Phase	76:84	arg1	Characterization					0:15	Characterization	0:15	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.	0:85	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	1	28	theme	collision	216:224	arg1	CCS					241:243	CCS	241:243	CCS	241:243	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	28	theme	collision	216:224	arg1	section					232:238	their collision cross section	210:238	their collision cross section (CCS)	210:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	4	29	theme	protonation	747:757	arg1	states					759:764	protonation states	747:764	protonation states	747:764	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	0	30	theme	N-glycans	59:67	arg1	Ensembles					35:43	Conformational Ensembles	20:43	Conformational Ensembles of Protonated N-glycans in the Gas-Phase	20:84	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	2	31	theme	conformational	326:339	arg1	differences					341:351	subtle conformational differences	319:351	subtle conformational differences	319:351	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	4	32	dep	determined	702:711	arg1	both					713:716	both	713:716	both	713:716	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	2	33	theme	subtle	319:324	arg1	differences					341:351	subtle conformational differences	319:351	subtle conformational differences	319:351	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	1	34	theme	cross	226:230	arg1	CCS					241:243	CCS	241:243	CCS	241:243	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	34	theme	cross	226:230	arg1	section					232:238	their collision cross section	210:238	their collision cross section (CCS)	210:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	2	35	theme	Recent	247:252	arg1	advances					254:261	Recent advances	247:261	Recent advances in IM-MS	247:270	Recent advances in IM-MS allow us to separate carbohydrate isomers with subtle conformational differences, but the relationship between CCS and atomic structure remains elusive.					
29374210	3	36	theme	molecular	545:553	arg1	simulations					564:574	molecular dynamics simulations	545:574	molecular dynamics simulations with enhanced sampling	545:597	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	3	37	theme	N-glycans	485:493	arg1	ensembles					462:470	conformational ensembles	447:470	conformational ensembles of gas-phase N-glycans	447:493	Here, we characterize conformational ensembles of gas-phase N-glycans under the electrospray ionization condition using molecular dynamics simulations with enhanced sampling.					
29374210	5	38	theme	CCS	804:806	arg1	basis					795:799	a physicochemical basis	777:799	a physicochemical basis of CCS for N-glycans	777:820	Providing a physicochemical basis of CCS for N-glycans helps not only to interpret IM-MS measurements but also to estimate CCSs of complex glycans.					
29374210	0	39	from	Ensembles	35:43	arg1	Gas-Phase					76:84	the Gas-Phase	72:84	the Gas-Phase	72:84	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	0	40	theme	Conformational	20:33	arg1	Ensembles					35:43	Conformational Ensembles	20:43	Conformational Ensembles of Protonated N-glycans in the Gas-Phase	20:84	Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.					
29374210	4	41	theme	CCSs	631:634	arg1	separation					617:626	the separation	613:626	the separation of CCSs between isomers	613:650	We show that the separation of CCSs between isomers reflects folding features of N-glycans, which are determined both by chemical compositions and protonation states.					
29374210	1	42	theme	capable	141:147	arg1	spectrometry					105:116	Ion mobility mass spectrometry	87:116	Ion mobility mass spectrometry (IM-MS)	87:124	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
29374210	1	42	theme	capable	141:147	arg1	technique					131:139	a technique	129:139	a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS)	129:244	Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).					
30782654	9	0	theme	GspA	1849:1852	arg1	conservation					1824:1835	the conservation	1820:1835	the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria	1820:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	11	1	theme	catalytic	2393:2401	arg1	residues					2407:2414	conserved catalytic Arg residues	2383:2414	conserved catalytic Arg residues	2383:2414	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	6	2	theme	LcpA	1139:1142	arg1	stability					1144:1152	LcpA stability	1139:1152	LcpA stability	1139:1152	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	1	3	from	bacteria	215:222	arg1	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	1	4	theme	wall	268:271	arg1	acid					282:285	wall teichoic acid	268:285	wall teichoic acid	268:285	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	13	5	theme	wall-anchored	2709:2721	arg1	protein					2723:2729	a cell wall-anchored protein	2702:2729	a cell wall-anchored protein	2702:2729	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	7	6	theme	LcpA	1355:1358	arg1	stability					1360:1368	LcpA stability	1355:1368	LcpA stability	1355:1368	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	9	7	theme	Gram-positive	1903:1915	arg1	bacteria					1917:1924	Gram-positive bacteria	1903:1924	Gram-positive bacteria	1903:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	8	theme	oris	1677:1680	arg1	enzyme					1708:1713	an archetypal LCP enzyme	1690:1713	an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1690:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	8	theme	oris	1677:1680	arg1	LcpA					1682:1685	A. oris LcpA	1674:1685	A. oris LcpA	1674:1685	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	7	9	from	folding	1238:1244	arg1	oris					1252:1255	A. oris	1249:1255	A. oris	1249:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	9	10	dep	phosphotransfer	1609:1623	arg1	support					1661:1667	support	1661:1667	support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1661:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	0	11	theme	Wall-Anchored	116:128	arg1	Protein					130:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	13	12	from	glycosylation	2685:2697	arg1	bacteria					2748:2755	Gram-positive bacteria	2734:2755	Gram-positive bacteria	2734:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	9	13	theme	conserved	1931:1939	arg1	enzymes					1952:1958	the conserved LCP family enzymes	1927:1958	the conserved LCP family enzymes studied to date	1927:1974	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	14	theme	archetypal	1693:1702	arg1	enzyme					1708:1713	an archetypal LCP enzyme	1690:1713	an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1690:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	14	theme	archetypal	1693:1702	arg1	LcpA					1682:1685	A. oris LcpA	1674:1685	A. oris LcpA	1674:1685	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	8	15	theme	recombinant	1413:1423	arg1	enzyme					1430:1435	the recombinant LcpA enzyme	1409:1435	the recombinant LcpA enzyme	1409:1435	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	7	16	theme	oxidoreductase	1307:1320	arg1	VKOR					1322:1325	the thiol-disulfide oxidoreductase VKOR	1287:1325	the thiol-disulfide oxidoreductase VKOR	1287:1325	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	9	17	theme	family	1945:1950	arg1	enzymes					1952:1958	the conserved LCP family enzymes	1927:1958	the conserved LCP family enzymes studied to date	1927:1974	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	5	18	theme	lcpA	998:1001	arg1	mutant					1003:1008	the lcpA mutant	994:1008	the lcpA mutant	994:1008	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	1	19	from	enzymes	190:196	arg1	bacteria					215:222	Gram-positive bacteria	201:222	Gram-positive bacteria	201:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	5	20	theme	GspA	884:887	arg1	glycosylation					889:901	GspA glycosylation	884:901	GspA glycosylation	884:901	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	10	21	theme	proteins	2128:2135	arg1	glycosylation					2103:2115	glycosylation	2103:2115	glycosylation of surface proteins	2103:2135	It is unknown if these enzymes catalyze glycosylation of surface proteins.					
30782654	3	22	gly	glycoprotein	525:536	arg1	GspA					517:520	GspA	517:520	GspA	517:520	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	22	gly	glycoprotein	525:536	arg1	glycoprotein					525:536	a glycoprotein	523:536	a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA	523:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	12	23	theme	protein	2545:2551	arg1	GspA					2553:2556	the surface protein GspA	2533:2556	the surface protein GspA	2533:2556	Importantly, LcpA mediates glycosylation of the surface protein GspA via phosphotransferase activity.					
30782654	1	24	from	family	180:185	arg1	bacteria					215:222	Gram-positive bacteria	201:222	Gram-positive bacteria	201:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	4	25	theme	structural	695:704	arg1	fold					706:709	an α-β-α structural fold	686:709	an α-β-α structural fold	686:709	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	8	26	theme	pyrophosphatase	1447:1461	arg1	activity					1463:1470	pyrophosphatase activity	1447:1470	pyrophosphatase activity	1447:1470	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	11	27	theme	disulfide	2442:2450	arg1	bond					2452:2455	a unique disulfide bond	2433:2455	a unique disulfide bond critical for protein stability	2433:2486	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	13	28	theme	archetypal	2649:2658	arg1	enzyme					2664:2669	an archetypal LCP enzyme	2646:2669	an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2646:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	1	29	theme	conserved	150:158	arg1	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	30	theme	cell	1735:1738	arg1	protein					1754:1760	a cell wall-anchored protein	1733:1760	a cell wall-anchored protein	1733:1760	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	30	theme	cell	1735:1738	arg1	process					1765:1771	a process	1763:1771	a process that may be conserved in Actinobacteria	1763:1811	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	11	31	theme	protein	2470:2476	arg1	stability					2478:2486	protein stability	2470:2486	protein stability	2470:2486	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	13	32	theme	enzyme	2664:2669	arg1	evidence					2634:2641	the first experimental evidence	2611:2641	the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2611:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	3	33	attach	attached	557:564	arg1	peptidoglycan					583:595	the bacterial peptidoglycan	569:595	the bacterial peptidoglycan	569:595	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	33	attach	attached	557:564	arg2	glycoprotein					525:536	a glycoprotein	523:536	a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA	523:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	33	attach	attached	557:564	arg3	SrtA					625:628	the housekeeping sortase SrtA	600:628	the housekeeping sortase SrtA	600:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	33	attach	attached	557:564	arg2	GspA					517:520	GspA	517:520	GspA	517:520	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	11	34	theme	biochemical	2220:2230	arg1	analyses					2232:2239	biochemical analyses	2220:2239	biochemical analyses	2220:2239	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	11	35	theme	critical	2457:2464	arg1	bond					2452:2455	a unique disulfide bond	2433:2455	a unique disulfide bond critical for protein stability	2433:2486	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	4	36	theme	conserved	724:732	arg1	domain					738:743	the conserved LCP domain	720:743	the conserved LCP domain	720:743	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	13	37	theme	experimental	2621:2632	arg1	evidence					2634:2641	the first experimental evidence	2611:2641	the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2611:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	5	38	theme	intermediate	934:945	arg1	form					947:950	an intermediate form	931:950	an intermediate form termed GspALMM	931:965	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	8	39	theme	diphosphate	1496:1506	arg1	bonds					1508:1512	diphosphate bonds	1496:1512	diphosphate bonds	1496:1512	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	0	40	theme	Bacterial	101:109	arg1	Protein					130:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	9	41	theme	wall	2021:2024	arg1	acid					2035:2038	wall teichoic acid	2021:2038	wall teichoic acid	2021:2038	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	1	42	theme	teichoic	273:280	arg1	acid					282:285	wall teichoic acid	268:285	wall teichoic acid	268:285	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	7	43	from	role	1184:1187	arg1	folding					1238:1244	oxidative protein folding	1220:1244	oxidative protein folding in A. oris	1220:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	6	44	theme	other	1018:1022	arg1	proteins					1028:1035	other LCP proteins	1018:1035	other LCP proteins characterized to date	1018:1057	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	11	45	theme	oris	2249:2252	arg1	LcpA					2254:2257	A. oris LcpA	2246:2257	A. oris LcpA	2246:2257	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	11	45	theme	oris	2249:2252	arg1	homolog					2269:2275	an LCP homolog	2262:2275	an LCP homolog	2262:2275	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	2	46	theme	surface	402:408	arg1	proteins					410:417	surface proteins	402:417	surface proteins	402:417	However, it is undetermined if these enzymes are capable of catalyzing glycan attachment to surface proteins.					
30782654	9	47	from	GspA	1581:1584	arg1	solution					1589:1596	solution	1589:1596	solution	1589:1596	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	4	48	theme	arginine	785:792	arg1	residues					794:801	characteristic catalytic arginine residues	760:801	characteristic catalytic arginine residues	760:801	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	3	49	theme	actinobacterium	427:441	arg1	oris					455:458	the actinobacterium Actinomyces oris	423:458	the actinobacterium Actinomyces oris	423:458	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	0	50	theme	LcpA	27:30	arg1	Mechanism					14:22	Mechanism	14:22	Mechanism	14:22	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	0	50	theme	LcpA	27:30	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	0	50	theme	LcpA	27:30	arg1	Phosphotransferase					35:52	a Phosphotransferase	33:52	a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein	33:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	7	51	theme	A.	1249:1250	arg1	oris					1252:1255	A. oris	1249:1255	A. oris	1249:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	9	52	theme	cell	2048:2051	arg1	envelope					2053:2060	the cell envelope	2044:2060	the cell envelope	2044:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	6	53	theme	stabilizing	1076:1086	arg1	bond					1098:1101	a stabilizing disulfide bond	1074:1101	a stabilizing disulfide bond	1074:1101	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	11	54	theme	pyrophosphatase	2289:2303	arg1	activities					2328:2337	pyrophosphatase and phosphotransferase activities	2289:2337	activities	2328:2337	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	8	55	theme	Biochemical	1371:1381	arg1	studies					1383:1389	Biochemical studies	1371:1389	Biochemical studies	1371:1389	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	5	56	located	observed	982:989	arg1	mutant					1003:1008	the lcpA mutant	994:1008	the lcpA mutant	994:1008	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	5	56	located	observed	982:989	arg2	accumulation					915:926	accumulation	915:926	accumulation	915:926	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	7	57	theme	oxidative	1220:1228	arg1	folding					1238:1244	oxidative protein folding	1220:1244	oxidative protein folding in A. oris	1220:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	12	58	theme	GspA	2553:2556	arg1	glycosylation					2516:2528	glycosylation	2516:2528	glycosylation of the surface protein GspA	2516:2556	Importantly, LcpA mediates glycosylation of the surface protein GspA via phosphotransferase activity.					
30782654	3	59	theme	Actinomyces	443:453	arg1	oris					455:458	the actinobacterium Actinomyces oris	423:458	the actinobacterium Actinomyces oris	423:458	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	4	60	theme	characteristic	760:773	arg1	residues					794:801	characteristic catalytic arginine residues	760:801	characteristic catalytic arginine residues	760:801	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	7	61	theme	formation	1207:1215	arg1	role					1184:1187	the established role	1168:1187	the established role of disulfide bond formation in oxidative protein folding in A. oris	1168:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	11	62	theme	Actinomyces	2174:2184	arg1	oris					2186:2189	the actinobacterium Actinomyces oris	2154:2189	the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses	2154:2239	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	13	63	theme	Gram-positive	2734:2746	arg1	bacteria					2748:2755	Gram-positive bacteria	2734:2755	Gram-positive bacteria	2734:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	7	64	theme	disulfide	1192:1200	arg1	formation					1207:1215	disulfide bond formation	1192:1215	disulfide bond formation	1192:1215	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	11	65	theme	phosphotransferase	2309:2326	arg1	activities					2328:2337	pyrophosphatase and phosphotransferase activities	2289:2337	activities	2328:2337	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	9	66	theme	recombinant	1533:1543	arg1	enzyme					1545:1550	this recombinant enzyme	1528:1550	this recombinant enzyme	1528:1550	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	8	67	contain	possesses	1437:1445	arg2	activity					1463:1470	pyrophosphatase activity	1447:1470	pyrophosphatase activity	1447:1470	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	8	67	contain	possesses	1437:1445	arg1	enzyme					1430:1435	the recombinant LcpA enzyme	1409:1435	the recombinant LcpA enzyme	1409:1435	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	9	68	theme	high-GC-content	1863:1877	arg1	organisms.IMPORTANCE					1879:1898	these high-GC-content organisms.IMPORTANCE	1857:1898	these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria	1857:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	11	69	theme	A.	2246:2247	arg1	LcpA					2254:2257	A. oris LcpA	2246:2257	A. oris LcpA	2246:2257	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	11	69	theme	A.	2246:2247	arg1	homolog					2269:2275	an LCP homolog	2262:2275	an LCP homolog	2262:2275	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	6	70	theme	disulfide	1088:1096	arg1	bond					1098:1101	a stabilizing disulfide bond	1074:1101	a stabilizing disulfide bond	1074:1101	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	13	71	theme	cell	2704:2707	arg1	protein					2723:2729	a cell wall-anchored protein	2702:2729	a cell wall-anchored protein	2702:2729	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	1	72	theme	LCP	175:177	arg1	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	12	73	gly	glycosylation	2516:2528	arg1	GspA					2553:2556	the surface protein GspA	2533:2556	the surface protein GspA	2533:2556	Importantly, LcpA mediates glycosylation of the surface protein GspA via phosphotransferase activity.					
30782654	13	74	theme	protein	2723:2729	arg1	glycosylation					2685:2697	glycosylation	2685:2697	glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2685:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	9	75	theme	A.	1674:1675	arg1	enzyme					1708:1713	an archetypal LCP enzyme	1690:1713	an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1690:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	75	theme	A.	1674:1675	arg1	LcpA					1682:1685	A. oris LcpA	1674:1685	A. oris LcpA	1674:1685	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	10	76	theme	surface	2120:2126	arg1	proteins					2128:2135	surface proteins	2120:2135	surface proteins	2120:2135	It is unknown if these enzymes catalyze glycosylation of surface proteins.					
30782654	0	77	theme	Protein	130:136	arg1	Glycosylation					68:80	Glycosylation	68:80	Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein	68:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	1	78	theme	enzymes	190:196	arg1	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	6	79	contain	contains	1065:1072	arg2	bond					1098:1101	a stabilizing disulfide bond	1074:1101	a stabilizing disulfide bond	1074:1101	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	6	79	contain	contains	1065:1072	arg1	LcpA					1060:1063	LcpA	1060:1063	LcpA	1060:1063	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	6	80	theme	bond	1098:1101	arg1	mutations					1104:1112	mutations	1104:1112	mutations	1104:1112	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	11	81	theme	LCP	2265:2267	arg1	LcpA					2254:2257	A. oris LcpA	2246:2257	A. oris LcpA	2246:2257	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	11	81	theme	LCP	2265:2267	arg1	homolog					2269:2275	an LCP homolog	2262:2275	an LCP homolog	2262:2275	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	5	82	theme	alanine	818:824	arg1	R266					868:871	R266	868:871	R266	868:871	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	5	82	theme	alanine	818:824	arg1	R149					859:862	R149	859:862	R149	859:862	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	5	82	theme	alanine	818:824	arg1	substitution					826:837	alanine substitution	818:837	alanine substitution for these residues	818:856	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	12	83	theme	surface	2537:2543	arg1	GspA					2553:2556	the surface protein GspA	2533:2556	the surface protein GspA	2533:2556	Importantly, LcpA mediates glycosylation of the surface protein GspA via phosphotransferase activity.					
30782654	8	84	theme	LcpA	1425:1428	arg1	enzyme					1430:1435	the recombinant LcpA enzyme	1409:1435	the recombinant LcpA enzyme	1409:1435	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	9	85	theme	LCP	1941:1943	arg1	enzymes					1952:1958	the conserved LCP family enzymes	1927:1958	the conserved LCP family enzymes studied to date	1927:1974	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	13	86	theme	first	2615:2619	arg1	evidence					2634:2641	the first experimental evidence	2611:2641	the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2611:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	1	87	attach	attach	236:241	arg1	envelope					300:307	the cell envelope	291:307	the cell envelope	291:307	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	1	87	attach	attach	236:241	arg2	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	88	gly	glycosylates	1720:1731	arg1	protein					1754:1760	a cell wall-anchored protein	1733:1760	a cell wall-anchored protein	1733:1760	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	88	gly	glycosylates	1720:1731	arg1	process					1765:1771	a process	1763:1771	a process that may be conserved in Actinobacteria	1763:1811	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	89	theme	LCP	1704:1706	arg1	enzyme					1708:1713	an archetypal LCP enzyme	1690:1713	an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1690:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	89	theme	LCP	1704:1706	arg1	LcpA					1682:1685	A. oris LcpA	1674:1685	A. oris LcpA	1674:1685	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	3	90	theme	sortase	617:623	arg1	SrtA					625:628	the housekeeping sortase SrtA	600:628	the housekeeping sortase SrtA	600:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	7	91	with	line	1158:1161	arg1	role					1184:1187	the established role	1168:1187	the established role of disulfide bond formation in oxidative protein folding in A. oris	1168:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	11	92	theme	Arg	2403:2405	arg1	residues					2407:2414	conserved catalytic Arg residues	2383:2414	conserved catalytic Arg residues	2383:2414	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	9	93	dep	protein	1754:1760	arg1	known					1980:1984	known	1980:1984	are known to attach glycopolymers, including wall teichoic acid, to the cell envelope	1976:2060	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	13	94	gly	glycosylation	2685:2697	arg1	bacteria					2748:2755	Gram-positive bacteria	2734:2755	Gram-positive bacteria	2734:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	13	94	gly	glycosylation	2685:2697	arg1	protein					2723:2729	a cell wall-anchored protein	2702:2729	a cell wall-anchored protein	2702:2729	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	7	95	theme	thiol-disulfide	1291:1305	arg1	VKOR					1322:1325	the thiol-disulfide oxidoreductase VKOR	1287:1325	the thiol-disulfide oxidoreductase VKOR	1287:1325	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	11	96	theme	unique	2435:2440	arg1	bond					2452:2455	a unique disulfide bond	2433:2455	a unique disulfide bond critical for protein stability	2433:2486	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	4	97	theme	α-β-α	689:693	arg1	fold					706:709	an α-β-α structural fold	686:709	an α-β-α structural fold	686:709	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	7	98	theme	established	1172:1182	arg1	role					1184:1187	the established role	1168:1187	the established role of disulfide bond formation in oxidative protein folding in A. oris	1168:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	3	99	theme	bacterial	573:581	arg1	peptidoglycan					583:595	the bacterial peptidoglycan	569:595	the bacterial peptidoglycan	569:595	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	11	100	theme	X-ray	2194:2198	arg1	crystallography					2200:2214	X-ray crystallography	2194:2214	X-ray crystallography	2194:2214	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	9	101	from	organisms.IMPORTANCE	1879:1898	arg1	bacteria					1917:1924	Gram-positive bacteria	1903:1924	Gram-positive bacteria	1903:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	4	102	theme	X-ray	647:651	arg1	crystallography					653:667	X-ray crystallography	647:667	X-ray crystallography	647:667	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	13	103	theme	LCP	2660:2662	arg1	enzyme					2664:2669	an archetypal LCP enzyme	2646:2669	an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria	2646:2755	Our studies provide the first experimental evidence of an archetypal LCP enzyme that promotes glycosylation of a cell wall-anchored protein in Gram-positive bacteria.					
30782654	1	104	theme	LytR-CpsA-Psr	160:172	arg1	family					180:185	The widely conserved LytR-CpsA-Psr (LCP) family	139:185	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria	139:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	105	theme	wall-anchored	1740:1752	arg1	protein					1754:1760	a cell wall-anchored protein	1733:1760	a cell wall-anchored protein	1733:1760	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	9	105	theme	wall-anchored	1740:1752	arg1	process					1765:1771	a process	1763:1771	a process that may be conserved in Actinobacteria	1763:1811	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	11	106	theme	LCP	2358:2360	arg1	enzymes					2362:2368	LCP enzymes	2358:2368	LCP enzymes that require conserved catalytic Arg residues	2358:2414	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	0	107	theme	Gram-Positive	87:99	arg1	Protein					130:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	9	108	from	conservation	1824:1835	arg1	organisms.IMPORTANCE					1879:1898	these high-GC-content organisms.IMPORTANCE	1857:1898	these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria	1857:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	8	109	theme	bonds	1508:1512	arg1	hydrolysis					1482:1491	hydrolysis	1482:1491	hydrolysis of diphosphate bonds	1482:1512	Biochemical studies demonstrated that the recombinant LcpA enzyme possesses pyrophosphatase activity, enabling hydrolysis of diphosphate bonds.					
30782654	0	110	theme	Cell	111:114	arg1	Protein					130:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	0	111	gly	Glycosylation	68:80	arg1	Protein					130:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	a Gram-Positive Bacterial Cell Wall-Anchored Protein	85:136	Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.					
30782654	7	112	theme	vkor	1270:1273	arg1	deletion					1258:1265	deletion	1258:1265	deletion	1258:1265	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	11	113	theme	conserved	2383:2391	arg1	residues					2407:2414	conserved catalytic Arg residues	2383:2414	conserved catalytic Arg residues	2383:2414	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	4	114	theme	LCP	734:736	arg1	domain					738:743	the conserved LCP domain	720:743	the conserved LCP domain	720:743	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	6	115	theme	LCP	1024:1026	arg1	proteins					1028:1035	other LCP proteins	1018:1035	other LCP proteins characterized to date	1018:1057	Unlike other LCP proteins characterized to date, LcpA contains a stabilizing disulfide bond, mutations of which severely affect LcpA stability.					
30782654	9	116	theme	teichoic	2026:2033	arg1	acid					2035:2038	wall teichoic acid	2021:2038	wall teichoic acid	2021:2038	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	5	117	theme	form	947:950	arg1	accumulation					915:926	accumulation	915:926	accumulation	915:926	Consistently, alanine substitution for these residues, R149 and R266, abrogates GspA glycosylation, leading to accumulation of an intermediate form termed GspALMM, which is also observed in the lcpA mutant.					
30782654	4	118	theme	akin	712:715	arg1	fold					706:709	an α-β-α structural fold	686:709	an α-β-α structural fold	686:709	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	1	119	theme	Gram-positive	201:213	arg1	bacteria					215:222	Gram-positive bacteria	201:222	Gram-positive bacteria	201:222	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	3	120	attach	linked	507:512	arg1	GspA					517:520	GspA	517:520	GspA	517:520	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	120	attach	linked	507:512	arg1	glycoprotein					525:536	a glycoprotein	523:536	a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA	523:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	120	attach	linked	507:512	arg3	oris					455:458	the actinobacterium Actinomyces oris	423:458	the actinobacterium Actinomyces oris	423:458	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	3	120	attach	linked	507:512	arg2	homolog					468:474	an LCP homolog	461:474	an LCP homolog here named LcpA	461:490	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	11	121	theme	actinobacterium	2158:2172	arg1	oris					2186:2189	the actinobacterium Actinomyces oris	2154:2189	the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses	2154:2239	We show here in the actinobacterium Actinomyces oris by X-ray crystallography and biochemical analyses that A. oris LcpA is an LCP homolog, possessing pyrophosphatase and phosphotransferase activities known to belong to LCP enzymes that require conserved catalytic Arg residues, while harboring a unique disulfide bond critical for protein stability.					
30782654	10	122	gly	glycosylation	2103:2115	arg1	proteins					2128:2135	surface proteins	2120:2135	surface proteins	2120:2135	It is unknown if these enzymes catalyze glycosylation of surface proteins.					
30782654	3	123	theme	housekeeping	604:615	arg1	SrtA					625:628	the housekeeping sortase SrtA	600:628	the housekeeping sortase SrtA	600:628	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	4	124	theme	catalytic	775:783	arg1	residues					794:801	characteristic catalytic arginine residues	760:801	characteristic catalytic arginine residues	760:801	Here we show by X-ray crystallography that LcpA adopts an α-β-α structural fold, akin to the conserved LCP domain, which harbors characteristic catalytic arginine residues.					
30782654	2	125	theme	glycan	381:386	arg1	attachment					388:397	glycan attachment	381:397	glycan attachment	381:397	However, it is undetermined if these enzymes are capable of catalyzing glycan attachment to surface proteins.					
30782654	1	126	theme	cell	295:298	arg1	envelope					300:307	the cell envelope	291:307	the cell envelope	291:307	The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30782654	7	127	theme	protein	1230:1236	arg1	folding					1238:1244	oxidative protein folding	1220:1244	oxidative protein folding in A. oris	1220:1255	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	12	128	theme	phosphotransferase	2562:2579	arg1	activity					2581:2588	phosphotransferase activity	2562:2588	phosphotransferase activity	2562:2588	Importantly, LcpA mediates glycosylation of the surface protein GspA via phosphotransferase activity.					
30782654	3	129	theme	LCP	464:466	arg1	homolog					468:474	an LCP homolog	461:474	an LCP homolog here named LcpA	461:490	In the actinobacterium Actinomyces oris, an LCP homolog here named LcpA is genetically linked to GspA, a glycoprotein that is covalently attached to the bacterial peptidoglycan by the housekeeping sortase SrtA.					
30782654	7	130	theme	bond	1202:1205	arg1	formation					1207:1215	disulfide bond formation	1192:1215	disulfide bond formation	1192:1215	In line with the established role of disulfide bond formation in oxidative protein folding in A. oris, deletion of vkor, coding for the thiol-disulfide oxidoreductase VKOR, also significantly reduces LcpA stability.					
30782654	9	131	theme	LcpA	1840:1843	arg1	conservation					1824:1835	the conservation	1820:1835	the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria	1820:1924	Furthermore, this recombinant enzyme, which weakly interacts with GspA in solution, catalyzes phosphotransfer to GspALMM Altogether, the findings support that A. oris LcpA is an archetypal LCP enzyme that glycosylates a cell wall-anchored protein, a process that may be conserved in Actinobacteria, given the conservation of LcpA and GspA in these high-GC-content organisms.IMPORTANCE In Gram-positive bacteria, the conserved LCP family enzymes studied to date are known to attach glycopolymers, including wall teichoic acid, to the cell envelope.					
30199683	0	0	theme	extracellular	74:86	arg1	substances					98:107	extracellular polymeric substances	74:107	extracellular polymeric substances	74:107	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	7	1	theme	stress	1516:1521	arg1	resistance					1523:1532	stress resistance	1516:1532	stress resistance	1516:1532	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	4	2	theme	cold	878:881	arg1	Peru					909:912	Peru	909:912	Peru	909:912	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	2	theme	cold	878:881	arg1	areas					883:887	two cold areas	874:887	two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica)	874:942	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	3	theme	diverse	725:731	arg1	biofilms					733:740	20 phylogenetically diverse biofilms	705:740	20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain)	705:992	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	2	4	from	present	300:306	arg1	biofilms					311:318	biofilms	311:318	biofilms	311:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	3	5	theme	suitable	513:520	arg1	characteristics					530:544	suitable biofilm characteristics	513:544	suitable biofilm characteristics	513:544	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	0	6	theme	substances	98:107	arg1	composition					59:69	the composition	55:69	the composition of extracellular polymeric substances in microbial mats from extreme environments	55:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	4	7	theme	geothermal	818:827	arg1	areas					829:833	two geothermal areas	814:833	two geothermal areas (Copahue, Argentina; Seltun, Iceland)	814:871	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	7	8	theme	heavy	1464:1468	arg1	toxicity					1476:1483	heavy metal toxicity	1464:1483	heavy metal toxicity	1464:1483	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	2	9	from	biofilms	311:318	arg1	present					300:306	present	300:306	present	300:306	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	0	10	theme	polymeric	88:96	arg1	substances					98:107	extracellular polymeric substances	74:107	extracellular polymeric substances	74:107	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	7	11	theme	metal	1470:1474	arg1	toxicity					1476:1483	heavy metal toxicity	1464:1483	heavy metal toxicity	1464:1483	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	6	12	theme	biofilm	1186:1192	arg1	characteristics					1194:1208	biofilm characteristics	1186:1208	biofilm characteristics	1186:1208	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	8	13	theme	ecological	1730:1739	arg1	adaptations					1741:1751	ecological adaptations	1730:1751	ecological adaptations in genetically modified microorganisms	1730:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	8	14	theme	extreme	1664:1670	arg1	environment					1672:1682	each extreme environment	1659:1682	each extreme environment	1659:1682	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	0	15	theme	microbial	112:120	arg1	mats					122:125	microbial mats	112:125	microbial mats from extreme environments	112:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	8	16	theme	conserved	1618:1626	arg1	patterns					1646:1653	conserved EPS compositional patterns	1618:1653	conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms	1618:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	5	17	theme	antibody	1097:1104	arg1	microarray					1106:1115	an antibody microarray	1094:1115	an antibody microarray	1094:1115	Biofilms were subjected to biochemical characterization, glycan profiling and immunoprofiling with an antibody microarray.					
30199683	6	18	theme	EPS	1275:1277	arg1	compositions					1279:1290	similar EPS compositions	1267:1290	similar EPS compositions regardless of the phylogeny of their main species	1267:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	4	19	dep	Peru	909:912	arg1	Antarctica					932:941	Antarctica	932:941	Antarctica	932:941	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	19	dep	Peru	909:912	arg1	Peninsula					921:929	Byers Peninsula	915:929	Byers Peninsula	915:929	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	20	dep	areas	829:833	arg1	Argentina					845:853	Argentina	845:853	Argentina	845:853	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	0	21	theme	Environmental	0:12	arg1	parameters					14:23	Environmental parameters	0:23	Environmental parameters	0:23	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	6	22	theme	similar	1267:1273	arg1	compositions					1279:1290	similar EPS compositions	1267:1290	similar EPS compositions regardless of the phylogeny of their main species	1267:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	6	23	theme	environmental	1142:1154	arg1	conditions					1156:1165	environmental conditions	1142:1165	environmental conditions	1142:1165	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	8	24	theme	modified	1768:1775	arg1	microorganisms					1777:1790	genetically modified microorganisms	1756:1790	genetically modified microorganisms	1756:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	5	25	theme	glycan	1052:1057	arg1	profiling					1059:1067	glycan profiling	1052:1067	glycan profiling	1052:1067	Biofilms were subjected to biochemical characterization, glycan profiling and immunoprofiling with an antibody microarray.					
30199683	7	26	theme	environmental	1416:1428	arg1	toxicity					1476:1483	heavy metal toxicity	1464:1483	heavy metal toxicity	1464:1483	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	7	26	theme	environmental	1416:1428	arg1	conditions					1430:1439	environmental conditions	1416:1439	environmental conditions such as temperature or heavy metal toxicity	1416:1483	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	7	26	theme	environmental	1416:1428	arg1	temperature					1449:1459	temperature	1449:1459	temperature	1449:1459	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	7	27	theme	monosaccharides	1369:1383	arg1	concentration					1347:1359	The concentration	1343:1359	The concentration of some monosaccharides in the EPS	1343:1394	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	7	27	theme	monosaccharides	1369:1383	arg1	related					1405:1411	related	1405:1411	related	1405:1411	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	4	28	theme	Byers	915:919	arg1	Peninsula					921:929	Byers Peninsula	915:929	Byers Peninsula	915:929	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	3	29	theme	important	563:571	arg1	process					573:579	an important process	560:579	an important process mediating the adaptation of microorganisms to novel environmental conditions	560:656	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	3	29	theme	important	563:571	arg1	acquisition					498:508	The acquisition	494:508	The acquisition of suitable biofilm characteristics	494:544	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	1	30	theme	key	193:195	arg1	trait					197:201	a key trait	191:201	a key trait for microorganisms growing in extreme environments	191:252	The ability to establish biofilms is a key trait for microorganisms growing in extreme environments.					
30199683	1	30	theme	key	193:195	arg1	ability					158:164	The ability to establish biofilms	154:186	The ability to establish biofilms	154:186	The ability to establish biofilms is a key trait for microorganisms growing in extreme environments.					
30199683	2	31	attach	present	300:306	arg2	substances					283:292	The extracellular polymeric substances	255:292	The extracellular polymeric substances (EPS) present in biofilms	255:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	31	attach	present	300:306	arg1	biofilms					311:318	biofilms	311:318	biofilms	311:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	31	attach	present	300:306	arg2	EPS					295:297	EPS	295:297	EPS	295:297	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	32	theme	present	300:306	arg1	EPS					295:297	EPS	295:297	EPS	295:297	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	32	theme	present	300:306	arg1	substances					283:292	The extracellular polymeric substances	255:292	The extracellular polymeric substances (EPS) present in biofilms	255:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	33	theme	metal	477:481	arg1	pollution					483:491	heavy metal pollution	471:491	heavy metal pollution	471:491	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	4	34	theme	biofilms	733:740	arg1	EPS					698:700	the EPS	694:700	the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain)	694:992	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	35	dep	Argentina	845:853	arg1	Iceland					864:870	Iceland	864:870	Iceland	864:870	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	3	36	theme	microorganisms	609:622	arg1	adaptation					595:604	the adaptation	591:604	the adaptation of microorganisms to novel environmental conditions	591:656	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	2	37	theme	stressors	412:420	arg1	kinds					389:393	all kinds	385:393	all kinds	385:393	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	37	theme	stressors	412:420	arg1	salinity					446:453	salinity	446:453	salinity	446:453	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	37	theme	stressors	412:420	arg1	pollution					483:491	heavy metal pollution	471:491	heavy metal pollution	471:491	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	37	theme	stressors	412:420	arg1	desiccation					433:443	desiccation	433:443	desiccation	433:443	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	37	theme	stressors	412:420	arg1	temperature					456:466	temperature	456:466	temperature	456:466	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	38	theme	heavy	471:475	arg1	pollution					483:491	heavy metal pollution	471:491	heavy metal pollution	471:491	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	0	39	theme	extreme	132:138	arg1	environments					140:151	extreme environments	132:151	extreme environments	132:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	0	40	from	composition	59:69	arg1	mats					122:125	microbial mats	112:125	microbial mats from extreme environments	112:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	0	40	from	composition	59:69	arg1	environments					140:151	extreme environments	132:151	extreme environments	132:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	8	41	theme	patterns	1646:1653	arg1	existence					1605:1613	the existence	1601:1613	the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms	1601:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	2	42	theme	environmental	398:410	arg1	stressors					412:420	environmental stressors	398:420	environmental stressors	398:420	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	7	43	theme	specific	1553:1560	arg1	sugars					1562:1567	specific sugars	1553:1567	specific sugars	1553:1567	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	6	44	theme	same	1240:1243	arg1	environment					1245:1255	the same environment	1236:1255	the same environment achieving similar EPS compositions regardless of the phylogeny of their main species	1236:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	6	45	theme	species	1334:1340	arg1	phylogeny					1310:1318	the phylogeny	1306:1318	the phylogeny of their main species	1306:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	2	46	theme	surface	337:343	arg1	attachment					345:354	surface attachment	337:354	surface attachment	337:354	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	6	47	theme	main	1329:1332	arg1	species					1334:1340	their main species	1323:1340	their main species	1323:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	2	48	theme	polymeric	273:281	arg1	EPS					295:297	EPS	295:297	EPS	295:297	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	48	theme	polymeric	273:281	arg1	substances					283:292	The extracellular polymeric substances	255:292	The extracellular polymeric substances (EPS) present in biofilms	255:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	8	49	from	adaptations	1741:1751	arg1	microorganisms					1777:1790	genetically modified microorganisms	1756:1790	genetically modified microorganisms	1756:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	4	50	theme	extreme	782:788	arg1	river					969:973	one extremely acidic river	948:973	one extremely acidic river (Río Tinto, Spain)	948:992	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	50	theme	extreme	782:788	arg1	areas					883:887	two cold areas	874:887	two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica)	874:942	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	50	theme	extreme	782:788	arg1	areas					829:833	two geothermal areas	814:833	two geothermal areas (Copahue, Argentina; Seltun, Iceland)	814:871	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	50	theme	extreme	782:788	arg1	environments					790:801	five contrasting extreme environments	765:801	five contrasting extreme environments	765:801	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	0	51	from	environments	140:151	arg1	mats					122:125	microbial mats	112:125	microbial mats from extreme environments	112:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	0	51	from	environments	140:151	arg1	composition					59:69	the composition	55:69	the composition of extracellular polymeric substances in microbial mats from extreme environments	55:151	Environmental parameters, and not phylogeny, determine the composition of extracellular polymeric substances in microbial mats from extreme environments.					
30199683	5	52	theme	biochemical	1022:1032	arg1	characterization					1034:1049	biochemical characterization	1022:1049	biochemical characterization	1022:1049	Biofilms were subjected to biochemical characterization, glycan profiling and immunoprofiling with an antibody microarray.					
30199683	2	53	theme	extracellular	259:271	arg1	EPS					295:297	EPS	295:297	EPS	295:297	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	2	53	theme	extracellular	259:271	arg1	substances					283:292	The extracellular polymeric substances	255:292	The extracellular polymeric substances (EPS) present in biofilms	255:318	The extracellular polymeric substances (EPS) present in biofilms provide not only surface attachment, but also protection against all kinds of environmental stressors, including desiccation, salinity, temperature or heavy metal pollution.					
30199683	4	54	theme	contrasting	770:780	arg1	river					969:973	one extremely acidic river	948:973	one extremely acidic river (Río Tinto, Spain)	948:992	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	54	theme	contrasting	770:780	arg1	areas					883:887	two cold areas	874:887	two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica)	874:942	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	54	theme	contrasting	770:780	arg1	areas					829:833	two geothermal areas	814:833	two geothermal areas (Copahue, Argentina; Seltun, Iceland)	814:871	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	54	theme	contrasting	770:780	arg1	environments					790:801	five contrasting extreme environments	765:801	five contrasting extreme environments	765:801	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	8	55	theme	EPS	1628:1630	arg1	patterns					1646:1653	conserved EPS compositional patterns	1618:1653	conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms	1618:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
30199683	4	56	theme	acidic	962:967	arg1	Spain					987:991	Spain	987:991	Spain	987:991	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	4	56	theme	acidic	962:967	arg1	river					969:973	one extremely acidic river	948:973	one extremely acidic river (Río Tinto, Spain)	948:992	In this work we have characterized the EPS of 20 phylogenetically diverse biofilms collected in situ from five contrasting extreme environments, including two geothermal areas (Copahue, Argentina; Seltun, Iceland), two cold areas (Pastoruri glacier, Peru; Byers Peninsula, Antarctica) and one extremely acidic river (Río Tinto, Spain).					
30199683	3	57	theme	novel	627:631	arg1	conditions					647:656	novel environmental conditions	627:656	novel environmental conditions	627:656	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	7	58	from	concentration	1347:1359	arg1	EPS					1392:1394	the EPS	1388:1394	the EPS	1388:1394	The concentration of some monosaccharides in the EPS could be related to environmental conditions such as temperature or heavy metal toxicity, suggesting that in some cases stress resistance can be mediated by specific sugars.					
30199683	3	59	theme	biofilm	522:528	arg1	characteristics					530:544	suitable biofilm characteristics	513:544	suitable biofilm characteristics	513:544	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	6	60	from	environment	1245:1255	arg1	microorganisms					1216:1229	microorganisms	1216:1229	microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species	1216:1340	Our results showed that environmental conditions strongly influence biofilm characteristics, with microorganisms from the same environment achieving similar EPS compositions regardless of the phylogeny of their main species.					
30199683	1	61	theme	extreme	233:239	arg1	environments					241:252	extreme environments	233:252	extreme environments	233:252	The ability to establish biofilms is a key trait for microorganisms growing in extreme environments.					
30199683	3	62	theme	environmental	633:645	arg1	conditions					647:656	novel environmental conditions	627:656	novel environmental conditions	627:656	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	3	63	theme	characteristics	530:544	arg1	process					573:579	an important process	560:579	an important process mediating the adaptation of microorganisms to novel environmental conditions	560:656	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	3	63	theme	characteristics	530:544	arg1	acquisition					498:508	The acquisition	494:508	The acquisition of suitable biofilm characteristics	494:544	The acquisition of suitable biofilm characteristics might thus be an important process mediating the adaptation of microorganisms to novel environmental conditions.					
30199683	8	64	theme	compositional	1632:1644	arg1	patterns					1646:1653	conserved EPS compositional patterns	1618:1653	conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms	1618:1790	Overall, our results highlight the existence of conserved EPS compositional patterns for each extreme environment, which could in turn be exploited to engineer ecological adaptations in genetically modified microorganisms.					
31291761	0	0	from	analysis	19:26	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	1	theme	high	145:148	arg1	processing					159:168	high pressure processing	145:168	high pressure processing	145:168	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	2	theme	wall-modifying	1074:1087	arg1	enzymes					1089:1095	cell wall-modifying enzymes	1069:1095	cell wall-modifying enzymes present in the primary cell walls	1069:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	4	3	theme	flowering	856:864	arg1	dicots					866:871	flowering dicots	856:871	flowering dicots	856:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	1	4	theme	cell	223:226	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	0	5	theme	oxidase	72:78	arg1	analysis					19:26	analysis	19:26	analysis	19:26	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	5	theme	oxidase	72:78	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	1	6	theme	fruit	309:313	arg1	varieties					315:323	two pawpaw (Asimina triloba) fruit varieties	280:323	two pawpaw (Asimina triloba) fruit varieties	280:323	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	6	theme	fruit	309:313	arg1	Belle					354:358	Belle	354:358	Belle	354:358	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	7	theme	pressure	389:396	arg1	processing					398:407	high pressure processing	384:407	high pressure processing	384:407	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	2	8	theme	postharvest	557:567	arg1	softening					576:584	pawpaw's deleterious postharvest tissue softening	536:584	pawpaw's deleterious postharvest tissue softening	536:584	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	1	9	theme	wall	228:231	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	0	10	from	Identification	0:13	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	2	11	theme	tissue	569:574	arg1	softening					576:584	pawpaw's deleterious postharvest tissue softening	536:584	pawpaw's deleterious postharvest tissue softening	536:584	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	5	12	theme	wall	912:915	arg1	structure					917:925	the fine cell wall structure	898:925	the fine cell wall structure	898:925	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	0	13	from	pulp	125:128	arg1	analysis					19:26	analysis	19:26	analysis	19:26	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	13	from	pulp	125:128	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	14	theme	pawpaw	83:88	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	15	theme	pressure	150:157	arg1	processing					159:168	high pressure processing	145:168	high pressure processing	145:168	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	6	16	theme	processing	1146:1155	arg1	treatment					1157:1165	High pressure processing treatment	1132:1165	High pressure processing treatment	1132:1165	High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.					
31291761	2	17	theme	pressure	471:478	arg1	processing					480:489	high pressure processing	466:489	high pressure processing	466:489	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	0	18	theme	refrigerated	174:185	arg1	storage					187:193	refrigerated storage	174:193	refrigerated storage	174:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	19	dep	varieties	939:947	arg1	inferred					956:963	inferred	956:963	can be inferred	949:963	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	6	20	theme	pressure	1137:1144	arg1	treatment					1157:1165	High pressure processing treatment	1132:1165	High pressure processing treatment	1132:1165	High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.					
31291761	4	21	theme	primary	834:840	arg1	walls					847:851	primary cell walls	834:851	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	2	22	theme	high	466:469	arg1	processing					480:489	high pressure processing	466:489	high pressure processing	466:489	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	0	23	from	wall	36:39	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	24	theme	present	1097:1103	arg1	enzymes					1089:1095	cell wall-modifying enzymes	1069:1095	cell wall-modifying enzymes present in the primary cell walls	1069:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	5	25	from	differences	883:893	arg1	structure					917:925	the fine cell wall structure	898:925	the fine cell wall structure	898:925	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	6	26	theme	High	1132:1135	arg1	treatment					1157:1165	High pressure processing treatment	1132:1165	High pressure processing treatment	1132:1165	High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.					
31291761	4	27	dep	type	827:830	arg1	walls					847:851	primary cell walls	834:851	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	3	28	theme	major	697:701	arg1	classes					703:709	most major classes	692:709	most major classes of cell wall glycans	692:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	6	29	theme	oxidase	1223:1229	arg1	activity					1231:1238	polyphenol oxidase activity	1212:1238	polyphenol oxidase activity	1212:1238	High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.					
31291761	3	30	theme	glycans	724:730	arg1	classes					703:709	most major classes	692:709	most major classes of cell wall glycans	692:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	6	31	theme	polyphenol	1212:1221	arg1	activity					1231:1238	polyphenol oxidase activity	1212:1238	polyphenol oxidase activity	1212:1238	High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.					
31291761	3	32	theme	epitopes	672:679	arg1	groups					655:660	19 groups	652:660	19 groups of glycan epitopes present in most major classes of cell wall glycans	652:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	3	32	theme	epitopes	672:679	arg1	epitopes					672:679	glycan epitopes	665:679	glycan epitopes present in most major classes of cell wall glycans	665:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	3	33	theme	glycan	665:670	arg1	epitopes					672:679	glycan epitopes	665:679	glycan epitopes present in most major classes of cell wall glycans	665:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	1	34	theme	polyphenol	249:258	arg1	activity					268:275	polyphenol oxidase activity	249:275	polyphenol oxidase activity	249:275	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	4	35	theme	typical	819:825	arg1	type					827:830	typical type I primary cell walls of flowering dicots	819:871	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	1	36	theme	refrigerated	424:435	arg1	storage					437:443	refrigerated storage	424:443	refrigerated storage	424:443	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	3	37	used	used	740:743	arg2	groupings					627:635	mAb groupings	623:635	mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition	623:778	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	3	38	from	classes	703:709	arg1	present					681:687	present	681:687	present	681:687	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	1	39	theme	oxidase	260:266	arg1	activity					268:275	polyphenol oxidase activity	249:275	polyphenol oxidase activity	249:275	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	2	40	theme	responsible	520:530	arg1	action					513:518	enzymatic action	503:518	enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning	503:597	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	1	41	theme	storage	437:443	arg1	processing					398:407	high pressure processing	384:407	high pressure processing	384:407	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	41	theme	storage	437:443	arg1	days					416:419	45 days	413:419	45 days of refrigerated storage	413:443	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	5	42	theme	cell	907:910	arg1	structure					917:925	the fine cell wall structure	898:925	the fine cell wall structure	898:925	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	5	43	theme	fine	902:905	arg1	structure					917:925	the fine cell wall structure	898:925	the fine cell wall structure	898:925	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	3	44	theme	cell	714:717	arg1	glycans					724:730	cell wall glycans	714:730	cell wall glycans	714:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	5	45	theme	cell	1069:1072	arg1	enzymes					1089:1095	cell wall-modifying enzymes	1069:1095	cell wall-modifying enzymes present in the primary cell walls	1069:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	0	46	theme	wall	36:39	arg1	analysis					19:26	analysis	19:26	analysis	19:26	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	46	theme	wall	36:39	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	3	47	theme	wall	719:722	arg1	glycans					724:730	cell wall glycans	714:730	cell wall glycans	714:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	2	48	theme	enzymatic	503:511	arg1	action					513:518	enzymatic action	503:518	enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning	503:597	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	0	49	from	oxidase	72:78	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	50	from	walls	1125:1129	arg1	present					1097:1103	present	1097:1103	present	1097:1103	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	0	51	theme	cell	31:34	arg1	wall					36:39	cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	31:193	wall	36:39	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	52	theme	primary	1112:1118	arg1	walls					1125:1129	the primary cell walls	1108:1129	the primary cell walls	1108:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	5	53	from	difference	1055:1064	arg1	enzymes					1089:1095	cell wall-modifying enzymes	1069:1095	cell wall-modifying enzymes present in the primary cell walls	1069:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	3	54	from	present	681:687	arg1	classes					703:709	most major classes	692:709	most major classes of cell wall glycans	692:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	1	55	theme	pawpaw	284:289	arg1	varieties					315:323	two pawpaw (Asimina triloba) fruit varieties	280:323	two pawpaw (Asimina triloba) fruit varieties	280:323	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	55	theme	pawpaw	284:289	arg1	Belle					354:358	Belle	354:358	Belle	354:358	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	2	56	theme	deleterious	545:555	arg1	softening					576:584	pawpaw's deleterious postharvest tissue softening	536:584	pawpaw's deleterious postharvest tissue softening	536:584	We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning.					
31291761	3	57	theme	mAb	623:625	arg1	groupings					627:635	mAb groupings	623:635	mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition	623:778	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	0	58	dep	wall	36:39	arg1	epitopes					48:55	glycan epitopes	41:55	glycan epitopes	41:55	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	3	59	theme	most	692:695	arg1	classes					703:709	most major classes	692:709	most major classes of cell wall glycans	692:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	5	60	attach	present	1097:1103	arg1	walls					1125:1129	the primary cell walls	1108:1129	the primary cell walls	1108:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	5	60	attach	present	1097:1103	arg2	enzymes					1089:1095	cell wall-modifying enzymes	1069:1095	cell wall-modifying enzymes present in the primary cell walls	1069:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	0	61	theme	glycan	41:46	arg1	epitopes					48:55	glycan epitopes	41:55	glycan epitopes	41:55	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	62	theme	fruit	119:123	arg1	pulp					125:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp	83:128	pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	83:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	1	63	dep	composition	233:243	arg1	the					219:221	the	219:221	the	219:221	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	3	64	theme	present	681:687	arg1	epitopes					672:679	glycan epitopes	665:679	glycan epitopes present in most major classes of cell wall glycans	665:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	1	65	theme	Asimina	292:298	arg1	varieties					315:323	two pawpaw (Asimina triloba) fruit varieties	280:323	two pawpaw (Asimina triloba) fruit varieties	280:323	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	65	theme	Asimina	292:298	arg1	Belle					354:358	Belle	354:358	Belle	354:358	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	66	theme	varieties	315:323	arg1	activity					268:275	polyphenol oxidase activity	249:275	polyphenol oxidase activity	249:275	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	66	theme	varieties	315:323	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	5	67	theme	refrigerated	1010:1021	arg1	storage					1023:1029	refrigerated storage	1010:1029	refrigerated storage	1010:1029	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	3	68	theme	Glycome	600:606	arg1	profiling					608:616	Glycome profiling	600:616	Glycome profiling	600:616	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	0	69	dep	pulp	125:128	arg1	Dunal					112:116	Dunal	112:116	Dunal	112:116	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	69	dep	pulp	125:128	arg1	affected					133:140	affected	133:140	affected by high pressure processing and refrigerated storage	133:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	0	69	dep	pulp	125:128	arg1	triloba					99:105	triloba	99:105	triloba	99:105	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	5	70	theme	cell	1120:1123	arg1	walls					1125:1129	the primary cell walls	1108:1129	the primary cell walls	1108:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	4	71	theme	dicots	866:871	arg1	type					827:830	typical type I primary cell walls of flowering dicots	819:871	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	4	72	theme	cell	842:845	arg1	walls					847:851	primary cell walls	834:851	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	0	73	theme	polyphenol	61:70	arg1	oxidase					72:78	polyphenol oxidase	61:78	polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage	61:193	Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.					
31291761	1	74	theme	high	384:387	arg1	processing					398:407	high pressure processing	384:407	high pressure processing	384:407	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	5	75	from	present	1097:1103	arg1	walls					1125:1129	the primary cell walls	1108:1129	the primary cell walls	1108:1129	However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls.					
31291761	4	76	contain	have	814:817	arg1	varieties					804:812	both varieties	799:812	both varieties	799:812	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	4	76	contain	have	814:817	arg2	type					827:830	typical type I primary cell walls of flowering dicots	819:871	typical type I primary cell walls of flowering dicots	819:871	Results show that both varieties have typical type I primary cell walls of flowering dicots.					
31291761	3	77	theme	cell	758:761	arg1	composition					768:778	cell wall composition	758:778	cell wall composition	758:778	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	1	78	theme	triloba	300:306	arg1	varieties					315:323	two pawpaw (Asimina triloba) fruit varieties	280:323	two pawpaw (Asimina triloba) fruit varieties	280:323	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	1	78	theme	triloba	300:306	arg1	Belle					354:358	Belle	354:358	Belle	354:358	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw (Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage.					
31291761	3	79	theme	wall	763:766	arg1	composition					768:778	cell wall composition	758:778	cell wall composition	758:778	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	3	80	attach	present	681:687	arg1	classes					703:709	most major classes	692:709	most major classes of cell wall glycans	692:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31291761	3	80	attach	present	681:687	arg2	epitopes					672:679	glycan epitopes	665:679	glycan epitopes present in most major classes of cell wall glycans	665:730	Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition.					
31096570	9	0	theme	glycans	1008:1014	arg1	presentation					992:1003	multivalent presentation	980:1003	multivalent presentation of glycans in the gel	980:1025	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	11	1	theme	infections	1376:1385	arg1	treatment					1363:1371	antibiotic treatment	1352:1371	antibiotic treatment of infections based on glycan interactions between host and pathogen	1352:1440	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	0	2	with	Interaction	42:52	arg1	Lectins					59:65	Lectins	59:65	Lectins	59:65	Functional Glyco-Nanogels for Multivalent Interaction with Lectins.					
31096570	8	3	theme	lectin	858:863	arg1	studies					873:879	lectin binding studies	858:879	lectin binding studies using model lectins	858:899	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	9	4	from	presentation	992:1003	arg1	gel					1023:1025	the gel	1019:1025	the gel	1019:1025	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	7	5	theme	reaction	746:753	arg1	parameters					755:764	reaction parameters	746:764	reaction parameters	746:764	The influence of reaction parameters like crosslinker type or stabilizer amount was investigated.					
31096570	8	6	theme	composition-dependent	921:941	arg1	inhibition					943:952	composition-dependent inhibition	921:952	composition-dependent inhibition of lectin binding	921:970	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	7	7	theme	stabilizer	791:800	arg1	amount					802:807	stabilizer amount	791:807	stabilizer amount	791:807	The influence of reaction parameters like crosslinker type or stabilizer amount was investigated.					
31096570	10	8	from	influence	1224:1232	arg1	secretion					1241:1249	the secretion	1237:1249	the secretion of virulence factors	1237:1270	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31096570	7	9	theme	crosslinker	771:781	arg1	type					783:786	crosslinker type	771:786	crosslinker type	771:786	The influence of reaction parameters like crosslinker type or stabilizer amount was investigated.					
31096570	11	10	theme	Functional	1273:1282	arg1	glycogels					1284:1292	Functional glycogels	1273:1292	Functional glycogels	1273:1292	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	5	11	theme	free	532:535	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	5	12	theme	glycomonomers	550:562	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	9	13	theme	glycan	1144:1149	arg1	loading					1151:1157	the glycan loading	1140:1157	the glycan loading	1140:1157	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	10	14	with	studies	1166:1172	arg1	aeruginosa					1191:1200	Pseudomonas aeruginosa	1179:1200	Pseudomonas aeruginosa	1179:1200	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31096570	9	15	theme	loading	1151:1157	arg1	determination					1123:1135	determination	1123:1135	determination of the glycan loading	1123:1157	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	8	16	theme	binding	865:871	arg1	studies					873:879	lectin binding studies	858:879	lectin binding studies using model lectins	858:899	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	6	17	theme	precipitation	639:651	arg1	polymerization					653:666	a precipitation polymerization	637:666	a precipitation polymerization	637:666	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	8	18	theme	binding	964:970	arg1	size					912:915	size	912:915	size	912:915	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	8	18	theme	binding	964:970	arg1	inhibition					943:952	composition-dependent inhibition	921:952	composition-dependent inhibition of lectin binding	921:970	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	11	19	theme	glycan	1396:1401	arg1	interactions					1403:1414	glycan interactions	1396:1414	glycan interactions between host and pathogen	1396:1440	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	4	20	dep	responsible	349:359	arg1	aeruginosa					337:346	Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections,	325:403	aeruginosa	337:346	Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers.					
31096570	0	21	theme	Functional	0:9	arg1	Glyco-Nanogels					11:24	Functional Glyco-Nanogels	0:24	Functional Glyco-Nanogels for Multivalent Interaction with Lectins	0:65	Functional Glyco-Nanogels for Multivalent Interaction with Lectins.					
31096570	3	22	theme	glycan-mediated	262:276	arg1	interactions					278:289	the glycan-mediated interactions	258:289	the glycan-mediated interactions between pathogens and host cells	258:322	Here, we emphasize the glycan-mediated interactions between pathogens and host cells.					
31096570	5	23	theme	microwave	496:504	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	0	24	theme	Multivalent	30:40	arg1	Interaction					42:52	Multivalent Interaction	30:52	Multivalent Interaction with Lectins	30:65	Functional Glyco-Nanogels for Multivalent Interaction with Lectins.					
31096570	10	25	theme	surprising	1213:1222	arg1	influence					1224:1232	a surprising influence	1211:1232	a surprising influence on the secretion of virulence factors	1211:1270	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31096570	3	26	theme	host	313:316	arg1	cells					318:322	host cells	313:322	host cells	313:322	Here, we emphasize the glycan-mediated interactions between pathogens and host cells.					
31096570	1	27	theme	tremendous	115:124	arg1	impact					126:131	tremendous impact	115:131	tremendous impact	115:131	Interactions between glycans and proteins have tremendous impact in biomolecular interactions.					
31096570	5	28	theme	assisted	506:513	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	7	29	theme	parameters	755:764	arg1	influence					733:741	The influence	729:741	The influence of reaction parameters like crosslinker type or stabilizer amount	729:807	The influence of reaction parameters like crosslinker type or stabilizer amount was investigated.					
31096570	5	30	theme	protecting	515:524	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	8	31	theme	lectin	957:962	arg1	binding					964:970	lectin binding	957:970	lectin binding	957:970	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	6	32	link	crosslinked	701:711	arg1	glyco-nanogels					713:726	crosslinked glyco-nanogels	701:726	crosslinked glyco-nanogels	701:726	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	11	33	theme	antibiotic	1352:1361	arg1	treatment					1363:1371	antibiotic treatment	1352:1371	antibiotic treatment of infections based on glycan interactions between host and pathogen	1352:1440	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	5	34	theme	group	526:530	arg1	synthesis					537:545	a microwave assisted protecting group free synthesis	494:545	a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose	494:601	We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose.					
31096570	8	35	theme	model	887:891	arg1	lectins					893:899	model lectins	887:899	model lectins	887:899	The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding.					
31096570	11	36	theme	future	1308:1313	arg1	alternatives					1322:1333	the future potent alternatives	1304:1333	the future potent alternatives	1304:1333	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	6	37	from	polymerization	653:666	arg1	polymerized					622:632	polymerized	622:632	polymerized	622:632	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	6	37	from	polymerization	653:666	arg1	monomers					608:615	The monomers	604:615	The monomers	604:615	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	9	38	theme	unmodified	1074:1083	arg1	saccharides					1085:1095	unmodified saccharides	1074:1095	unmodified saccharides	1074:1095	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	2	39	theme	cell-cell	186:194	arg1	interactions					196:207	cell-cell interactions	186:207	cell-cell interactions	186:207	They are important for cell-cell interactions, proliferation and much more.					
31096570	1	40	contain	have	110:113	arg2	impact					126:131	tremendous impact	115:131	tremendous impact	115:131	Interactions between glycans and proteins have tremendous impact in biomolecular interactions.					
31096570	1	40	contain	have	110:113	arg1	Interactions					68:79	Interactions	68:79	Interactions between glycans and proteins	68:108	Interactions between glycans and proteins have tremendous impact in biomolecular interactions.					
31096570	11	41	theme	potent	1315:1320	arg1	alternatives					1322:1333	the future potent alternatives	1304:1333	the future potent alternatives	1304:1333	Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.					
31096570	4	42	theme	infections	393:402	arg1	number					372:377	a huge number	365:377	a huge number of nosocomial infections	365:402	Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers.					
31096570	1	43	theme	biomolecular	136:147	arg1	interactions					149:160	biomolecular interactions	136:160	biomolecular interactions	136:160	Interactions between glycans and proteins have tremendous impact in biomolecular interactions.					
31096570	6	44	theme	crosslinked	701:711	arg1	glyco-nanogels					713:726	crosslinked glyco-nanogels	701:726	crosslinked glyco-nanogels	701:726	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	4	45	theme	nosocomial	382:391	arg1	infections					393:402	nosocomial infections	382:402	nosocomial infections	382:402	Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers.					
31096570	9	46	theme	multivalent	980:990	arg1	presentation					992:1003	multivalent presentation	980:1003	multivalent presentation of glycans in the gel	980:1025	Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading.					
31096570	6	47	from	polymerized	622:632	arg1	presence					675:682	the presence	671:682	the presence of NiPAm to form crosslinked glyco-nanogels	671:726	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	6	47	from	polymerized	622:632	arg1	polymerization					653:666	a precipitation polymerization	637:666	a precipitation polymerization	637:666	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	6	48	theme	NiPAm	687:691	arg1	presence					675:682	the presence	671:682	the presence of NiPAm to form crosslinked glyco-nanogels	671:726	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	10	49	theme	factors	1264:1270	arg1	secretion					1241:1249	the secretion	1237:1249	the secretion of virulence factors	1237:1270	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31096570	10	50	theme	First	1160:1164	arg1	studies					1166:1172	First studies	1160:1172	First studies with Pseudomonas aeruginosa	1160:1200	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31096570	6	51	from	presence	675:682	arg1	polymerized					622:632	polymerized	622:632	polymerized	622:632	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	6	51	from	presence	675:682	arg1	monomers					608:615	The monomers	604:615	The monomers	604:615	The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels.					
31096570	4	52	theme	huge	367:370	arg1	number					372:377	a huge number	365:377	a huge number of nosocomial infections	365:402	Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers.					
31096570	10	53	theme	virulence	1254:1262	arg1	factors					1264:1270	virulence factors	1254:1270	virulence factors	1254:1270	First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors.					
31525900	3	0	from	concentration	720:732	arg1	BGL1					707:710	BGL1	707:710	BGL1 at high concentration (200 μg mL-1)	707:746	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	6	1	theme	oligosaccharides	1403:1418	arg1	production					1367:1376	the production	1363:1376	the production of high value-added rare oligosaccharides	1363:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	6	2	dep	%	1204:1204	arg1	90					1202:1203	90	1202:1203	90	1202:1203	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	0	3	theme	Transglycosylation	82:99	arg1	Activity					101:108	a Distinctive Hydrolysis and Transglycosylation Activity	53:108	Activity	101:108	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	3	4	theme	200	735:737	arg1	μg					739:740	μg	739:740	μg	739:740	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	6	5	theme	rare	1398:1401	arg1	oligosaccharides					1403:1418	high value-added rare oligosaccharides	1381:1418	high value-added rare oligosaccharides	1381:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	5	6	dep	β	1021:1021	arg1	→					1025:1025	1 → 3	1023:1027	1 → 3	1023:1027	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	2	7	theme	activity	577:584	arg1	%					568:568	only 0.83 and 2.05%	550:568	only 0.83 and 2.05% of its activity toward laminaribiose	550:605	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	2	7	theme	activity	577:584	arg1	activity					577:584	its activity	573:584	its activity toward laminaribiose	573:605	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	3	8	theme	μg	739:740	arg1	mL-1					742:745	200 μg mL-1	735:745	200 μg mL-1	735:745	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	3	8	theme	μg	739:740	arg1	concentration					720:732	high concentration	715:732	high concentration (200 μg mL-1)	715:746	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	6	9	theme	value-added	1386:1396	arg1	oligosaccharides					1403:1418	high value-added rare oligosaccharides	1381:1418	high value-added rare oligosaccharides	1381:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	1	10	theme	hydrolytic	312:321	arg1	activity					323:330	hydrolytic activity	312:330	hydrolytic activity	312:330	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	6	11	theme	high	1381:1384	arg1	oligosaccharides					1403:1418	high value-added rare oligosaccharides	1381:1418	high value-added rare oligosaccharides	1381:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	5	12	from	laminarioligosaccharide	942:964	arg1	residue					919:925	one glucosyl residue	906:925	one glucosyl residue from substrate laminarioligosaccharide	906:964	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	6	13	theme	untreated	1267:1275	arg1	laminaritriose					1277:1290	untreated laminaritriose	1267:1290	untreated laminaritriose	1267:1290	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	5	14	theme	glucosyl	910:917	arg1	residue					919:925	one glucosyl residue	906:925	one glucosyl residue from substrate laminarioligosaccharide	906:964	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	6	15	theme	BGL1-digested	1149:1161	arg1	laminaritriose					1163:1176	The BGL1-digested laminaritriose	1145:1176	The BGL1-digested laminaritriose	1145:1176	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	1	16	theme	β-glucosidase	224:236	arg1	BGL1					238:241	β-glucosidase BGL1	224:241	β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea	224:300	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	5	17	from	acceptor	1007:1014	arg1	fashion					1042:1048	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	0	18	from	cinerea	36:42	arg1	BGL1					14:17	β-Glucosidase BGL1	0:17	β-Glucosidase BGL1 from Coprinopsis cinerea	0:42	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	4	19	theme	only	771:774	arg1	laminarioligosaccharides					776:799	only laminarioligosaccharides	771:799	only laminarioligosaccharides but not glucose, gentiobiose, and cellobiose	771:844	BGL1 utilizes only laminarioligosaccharides but not glucose, gentiobiose, and cellobiose to synthesize the higher oligosaccharides.					
31525900	0	20	theme	β-Glucosidase	0:12	arg1	BGL1					14:17	β-Glucosidase BGL1	0:17	β-Glucosidase BGL1 from Coprinopsis cinerea	0:42	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	3	21	theme	high	715:718	arg1	mL-1					742:745	200 μg mL-1	735:745	200 μg mL-1	735:745	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	3	21	theme	high	715:718	arg1	concentration					720:732	high concentration	715:732	high concentration (200 μg mL-1)	715:746	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	5	22	theme	β	1021:1021	arg1	fashion					1042:1048	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	2	23	theme	high	453:456	arg1	mL-1					480:483	200 μg mL-1	473:483	200 μg mL-1	473:483	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	2	23	theme	high	453:456	arg1	concentration					458:470	high concentration	453:470	high concentration (200 μg mL-1)	453:484	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	0	24	theme	Coprinopsis	24:34	arg1	cinerea					36:42	Coprinopsis cinerea	24:42	Coprinopsis cinerea	24:42	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	4	25	theme	higher	864:869	arg1	oligosaccharides					871:886	the higher oligosaccharides	860:886	the higher oligosaccharides	860:886	BGL1 utilizes only laminarioligosaccharides but not glucose, gentiobiose, and cellobiose to synthesize the higher oligosaccharides.					
31525900	6	26	theme	anti-oxidant	1225:1236	arg1	activity					1238:1245	the anti-oxidant activity	1221:1245	the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides	1221:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	3	27	from	concentration	649:661	arg1	BGL1					637:640	BGL1	637:640	BGL1 at low concentration (1.5 μg mL-1)	637:675	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	1	28	from	cinerea	294:300	arg1	BGL1					238:241	β-glucosidase BGL1	224:241	β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea	224:300	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	5	29	dep	β	1033:1033	arg1	→					1037:1037	1 → 6	1035:1039	1 → 6	1035:1039	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	6	30	theme	transglycosylation	1340:1357	arg1	application					1314:1324	a potential application	1302:1324	a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides	1302:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	1	31	theme	low	246:248	arg1	mL-1					271:274	15 μg mL-1	265:274	15 μg mL-1	265:274	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	1	31	theme	low	246:248	arg1	concentration					250:262	low concentration	246:262	low concentration (15 μg mL-1)	246:275	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	1	32	theme	15	265:266	arg1	μg					268:269	μg	268:269	μg	268:269	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	2	33	theme	200	473:475	arg1	μg					477:478	μg	477:478	μg	477:478	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	0	34	theme	3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides	147:193	arg1	Production					133:142	the Production	129:142	the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides	129:193	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	6	35	theme	BGL1-based	1329:1338	arg1	transglycosylation					1340:1357	BGL1-based transglycosylation	1329:1357	BGL1-based transglycosylation	1329:1357	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	2	36	from	concentration	458:470	arg1	BGL1					445:448	BGL1	445:448	BGL1 at high concentration (200 μg mL-1)	445:484	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	1	37	theme	μg	268:269	arg1	mL-1					271:274	15 μg mL-1	265:274	15 μg mL-1	265:274	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	1	37	theme	μg	268:269	arg1	concentration					250:262	low concentration	246:262	low concentration (15 μg mL-1)	246:275	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	0	38	theme	Distinctive	55:65	arg1	Hydrolysis					67:76	a Distinctive Hydrolysis and Transglycosylation Activity	53:108	Hydrolysis	67:76	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	3	39	theme	low	645:647	arg1	concentration					649:661	low concentration	645:661	low concentration (1.5 μg mL-1)	645:675	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	3	39	theme	low	645:647	arg1	mL-1					671:674	1.5 μg mL-1	664:674	1.5 μg mL-1	664:674	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	1	40	from	concentration	250:262	arg1	BGL1					238:241	β-glucosidase BGL1	224:241	β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea	224:300	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
31525900	6	41	dep	enhancement	1206:1216	arg1	%					1204:1204	%	1204:1204	%	1204:1204	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	5	42	theme	higher	1061:1066	arg1	laminarioligosaccharides					1068:1091	higher laminarioligosaccharides	1061:1091	higher laminarioligosaccharides	1061:1091	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	5	43	theme	β	1033:1033	arg1	fashion					1042:1048	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides	1019:1142	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	5	44	theme	substrate	932:940	arg1	laminarioligosaccharide					942:964	substrate laminarioligosaccharide	932:964	substrate laminarioligosaccharide	932:964	BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides.					
31525900	0	45	from	Application	114:124	arg1	Production					133:142	the Production	129:142	the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides	129:193	β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.					
31525900	6	46	from	enhancement	1206:1216	arg1	activity					1238:1245	the anti-oxidant activity	1221:1245	the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides	1221:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	2	47	theme	μg	477:478	arg1	mL-1					480:483	200 μg mL-1	473:483	200 μg mL-1	473:483	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	2	47	theme	μg	477:478	arg1	concentration					458:470	high concentration	453:470	high concentration (200 μg mL-1)	453:484	This study shows that BGL1 at high concentration (200 μg mL-1) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively.					
31525900	3	48	theme	1.5	664:666	arg1	μg					668:669	μg	668:669	μg	668:669	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	3	49	theme	μg	668:669	arg1	concentration					649:661	low concentration	645:661	low concentration (1.5 μg mL-1)	645:675	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	3	49	theme	μg	668:669	arg1	mL-1					671:674	1.5 μg mL-1	664:674	1.5 μg mL-1	664:674	Interestingly, BGL1 at low concentration (1.5 μg mL-1) showed transglycosylation but BGL1 at high concentration (200 μg mL-1) did not.					
31525900	6	50	theme	potential	1304:1312	arg1	application					1314:1324	a potential application	1302:1324	a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides	1302:1418	The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.					
31525900	1	51	theme	Coprinopsis	282:292	arg1	cinerea					294:300	Coprinopsis cinerea	282:300	Coprinopsis cinerea	282:300	We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL-1) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose.					
30513349	3	0	theme	infectious	691:700	arg1	particles					702:710	transmitting infectious particles	678:710	transmitting infectious particles	678:710	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	7	1	theme	N-linked	1123:1130	arg1	homogeneous					1175:1185	homogeneous	1175:1185	homogeneous	1175:1185	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	7	1	theme	N-linked	1123:1130	arg1	glyco-structures					1132:1147	the N-linked glyco-structures	1119:1147	the N-linked glyco-structures of rhA1AT and rhC1INH	1119:1169	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	2	2	theme	N-glycan	358:365	arg1	moieties					367:374	N-glycan moieties	358:374	N-glycan moieties	358:374	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	10	3	theme	unsafe	1851:1856	arg1	source					1866:1871	a limited and possibly unsafe protein source	1828:1871	a limited and possibly unsafe protein source for patients	1828:1884	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	6	4	theme	CRISPR/Cas9-mediated	1004:1023	arg1	disruption					1025:1034	CRISPR/Cas9-mediated disruption	1004:1034	CRISPR/Cas9-mediated disruption of 10 gene targets	1004:1053	This was achieved by combining CRISPR/Cas9-mediated disruption of 10 gene targets with overexpression of human ST6GAL1.					
30513349	7	5	theme	plasma-derived	1231:1244	arg1	A1AT					1246:1249	plasma-derived A1AT	1231:1249	plasma-derived A1AT	1231:1249	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	4	6	dep	rhA1AT	752:757	arg1	rhC1INH					760:766	rhC1INH	760:766	rhC1INH	760:766	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	10	7	theme	recombinant	1747:1757	arg1	versions					1759:1766	recombinant versions	1747:1766	recombinant versions	1747:1766	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	8	8	theme	in	1486:1487	arg1	activity					1495:1502	similar enzymatic in vitro activity	1468:1502	similar enzymatic in vitro activity	1468:1502	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	0	9	theme	N-glycosylation	109:123	arg1	profiles					125:132	fully humanized N-glycosylation profiles	93:132	fully humanized N-glycosylation profiles	93:132	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	4	10	theme	humanized	784:792	arg1	N-glycans					794:802	humanized N-glycans	784:802	humanized N-glycans	784:802	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	10	11	dep	envision	1693:1700	arg1	decreasing					1780:1789	decreasing	1780:1789	thereby decreasing our dependence on human donor blood	1772:1825	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	10	11	dep	envision	1693:1700	arg1	replacing					1702:1710	replacing	1702:1710	replacing plasma-derived A1AT and C1INH with recombinant versions	1702:1766	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	9	12	theme	glycoproteins	1636:1648	arg1	glycoproteins					1636:1648	glycoproteins	1636:1648	glycoproteins with fully humanized N-glycan profiles	1636:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	9	12	theme	glycoproteins	1636:1648	arg1	variety					1625:1631	a wider variety	1617:1631	a wider variety of glycoproteins with fully humanized N-glycan profiles	1617:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	8	13	theme	cell	1367:1370	arg1	line					1372:1375	our glyco-engineered cell line	1346:1375	our glyco-engineered cell line	1346:1375	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	9	14	theme	wider	1619:1623	arg1	glycoproteins					1636:1648	glycoproteins	1636:1648	glycoproteins with fully humanized N-glycan profiles	1636:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	9	14	theme	wider	1619:1623	arg1	variety					1625:1631	a wider variety	1617:1631	a wider variety of glycoproteins with fully humanized N-glycan profiles	1617:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	1	15	theme	N-glycosylation	272:286	arg1	profiles					288:295	N-glycosylation profiles	272:295	N-glycosylation profiles similar, but not identical, to humans	272:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	0	16	theme	humanized	99:107	arg1	profiles					125:132	fully humanized N-glycosylation profiles	93:132	fully humanized N-glycosylation profiles	93:132	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	8	17	theme	glyco-engineered	1350:1365	arg1	line					1372:1375	our glyco-engineered cell line	1346:1375	our glyco-engineered cell line	1346:1375	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	2	18	from	differences	343:353	arg1	moieties					367:374	N-glycan moieties	358:374	N-glycan moieties	358:374	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	5	19	theme	cell	887:890	arg1	lines					892:896	engineered CHO cell lines	872:896	engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles	872:970	Here, we present engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles.					
30513349	10	20	theme	donor	1815:1819	arg1	blood					1821:1825	human donor blood	1809:1825	human donor blood	1809:1825	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	4	21	theme	commercial	834:843	arg1	interest					845:852	clinical and commercial interest	821:852	clinical and commercial interest	821:852	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	9	22	theme	CHO	1596:1598	arg1	cells					1600:1604	CHO cells	1596:1604	CHO cells	1596:1604	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	8	23	from	counterparts	1435:1446	arg1	SDS-PAGE					1451:1458	SDS-PAGE	1451:1458	SDS-PAGE	1451:1458	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	7	24	theme	rhA1AT	1152:1157	arg1	homogeneous					1175:1185	homogeneous	1175:1185	homogeneous	1175:1185	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	7	24	theme	rhA1AT	1152:1157	arg1	glyco-structures					1132:1147	the N-linked glyco-structures	1119:1147	the N-linked glyco-structures of rhA1AT and rhC1INH	1119:1169	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	3	25	theme	particles	702:710	arg1	risk					670:673	a risk	668:673	a risk of transmitting infectious particles	668:710	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	0	26	theme	CHO	17:19	arg1	lines					26:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	10	27	theme	limited	1830:1836	arg1	source					1866:1871	a limited and possibly unsafe protein source	1828:1871	a limited and possibly unsafe protein source for patients	1828:1884	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	8	28	theme	detectable	1387:1396	arg1	differences					1398:1408	no detectable differences	1384:1408	no detectable differences	1384:1408	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	6	29	theme	ST6GAL1	1084:1090	arg1	overexpression					1060:1073	overexpression	1060:1073	overexpression of human ST6GAL1	1060:1090	This was achieved by combining CRISPR/Cas9-mediated disruption of 10 gene targets with overexpression of human ST6GAL1.					
30513349	0	30	theme	Glyco-engineered	0:15	arg1	lines					26:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	1	31	theme	similar	297:303	arg1	profiles					288:295	N-glycosylation profiles	272:295	N-glycosylation profiles similar, but not identical, to humans	272:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	10	32	from	dependence	1795:1804	arg1	blood					1821:1825	human donor blood	1809:1825	human donor blood	1809:1825	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	7	33	link	plasma-derived	1231:1244	arg1	A1AT					1246:1249	plasma-derived A1AT	1231:1249	plasma-derived A1AT	1231:1249	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	6	34	theme	human	1078:1082	arg1	ST6GAL1					1084:1090	human ST6GAL1	1078:1090	human ST6GAL1	1078:1090	This was achieved by combining CRISPR/Cas9-mediated disruption of 10 gene targets with overexpression of human ST6GAL1.					
30513349	8	35	theme	similar	1468:1474	arg1	activity					1495:1502	similar enzymatic in vitro activity	1468:1502	similar enzymatic in vitro activity	1468:1502	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	5	36	theme	humanized	937:945	arg1	profiles					963:970	fully humanized N-glycosylation profiles	931:970	fully humanized N-glycosylation profiles	931:970	Here, we present engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles.					
30513349	8	37	theme	plasma-purified	1419:1433	arg1	counterparts					1435:1446	their plasma-purified counterparts	1413:1446	their plasma-purified counterparts on SDS-PAGE	1413:1458	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	9	38	gly	glycoproteins	1636:1648	arg1	glycoproteins					1636:1648	glycoproteins	1636:1648	glycoproteins with fully humanized N-glycan profiles	1636:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	0	39	theme	cell	21:24	arg1	lines					26:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines	0:30	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	10	40	theme	human	1809:1813	arg1	blood					1821:1825	human donor blood	1809:1825	human donor blood	1809:1825	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	3	41	theme	cost-intensive	628:641	arg1	process					643:649	a cost-intensive process	626:649	a cost-intensive process	626:649	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	5	42	theme	N-glycosylation	947:961	arg1	profiles					963:970	fully humanized N-glycosylation profiles	931:970	fully humanized N-glycosylation profiles	931:970	Here, we present engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles.					
30513349	2	43	theme	protease	454:461	arg1	members					381:387	two members	377:387	two members of the serpin superfamily	377:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	43	theme	protease	454:461	arg1	C1INH					477:481	C1INH	477:481	C1INH	477:481	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	43	theme	protease	454:461	arg1	inhibitor					466:474	plasma protease C1 inhibitor	447:474	plasma protease C1 inhibitor (C1INH)	447:482	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	0	44	theme	alpha-1-antitrypsin	42:60	arg1	inhibitor					78:86	alpha-1-antitrypsin and C1 esterase inhibitor	42:86	inhibitor	78:86	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	8	45	contain	had	1464:1466	arg1	rhA1AT					1315:1320	rhA1AT	1315:1320	rhA1AT	1315:1320	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	8	45	contain	had	1464:1466	arg2	activity					1495:1502	similar enzymatic in vitro activity	1468:1502	similar enzymatic in vitro activity	1468:1502	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	7	46	link	N-linked	1123:1130	arg1	homogeneous					1175:1185	homogeneous	1175:1185	homogeneous	1175:1185	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	7	46	link	N-linked	1123:1130	arg1	glyco-structures					1132:1147	the N-linked glyco-structures	1119:1147	the N-linked glyco-structures of rhA1AT and rhC1INH	1119:1169	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	3	47	theme	therapeutic	574:584	arg1	proteins					586:593	therapeutic proteins	574:593	therapeutic proteins	574:593	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	2	48	theme	superfamily	403:413	arg1	alpha-1-antitrypsin					416:434	alpha-1-antitrypsin	416:434	alpha-1-antitrypsin	416:434	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	48	theme	superfamily	403:413	arg1	members					381:387	two members	377:387	two members of the serpin superfamily	377:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	48	theme	superfamily	403:413	arg1	inhibitor					466:474	plasma protease C1 inhibitor	447:474	plasma protease C1 inhibitor (C1INH)	447:482	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	49	theme	C1	463:464	arg1	members					381:387	two members	377:387	two members of the serpin superfamily	377:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	49	theme	C1	463:464	arg1	C1INH					477:481	C1INH	477:481	C1INH	477:481	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	49	theme	C1	463:464	arg1	inhibitor					466:474	plasma protease C1 inhibitor	447:474	plasma protease C1 inhibitor (C1INH)	447:482	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	1	50	theme	identical	314:322	arg1	profiles					288:295	N-glycosylation profiles	272:295	N-glycosylation profiles similar, but not identical, to humans	272:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	2	51	theme	human	512:516	arg1	plasma					518:523	human plasma	512:523	human plasma for treating A1AT and C1INH deficiency	512:562	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	10	52	theme	protein	1858:1864	arg1	source					1866:1871	a limited and possibly unsafe protein source	1828:1871	a limited and possibly unsafe protein source for patients	1828:1884	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	2	53	attach	derived	499:505	arg2	inhibitor					466:474	plasma protease C1 inhibitor	447:474	plasma protease C1 inhibitor (C1INH)	447:482	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	53	attach	derived	499:505	arg2	alpha-1-antitrypsin					416:434	alpha-1-antitrypsin	416:434	alpha-1-antitrypsin	416:434	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	53	attach	derived	499:505	arg1	plasma					518:523	human plasma	512:523	human plasma for treating A1AT and C1INH deficiency	512:562	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	53	attach	derived	499:505	arg2	members					381:387	two members	377:387	two members of the serpin superfamily	377:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	54	theme	serpin	396:401	arg1	superfamily					403:413	the serpin superfamily	392:413	the serpin superfamily	392:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	0	55	theme	C1	66:67	arg1	inhibitor					78:86	alpha-1-antitrypsin and C1 esterase inhibitor	42:86	inhibitor	78:86	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	9	56	with	glycoproteins	1636:1648	arg1	profiles					1680:1687	fully humanized N-glycan profiles	1655:1687	fully humanized N-glycan profiles	1655:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	4	57	theme	clinical	821:828	arg1	interest					845:852	clinical and commercial interest	821:852	clinical and commercial interest	821:852	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	9	58	theme	glyco-engineering	1566:1582	arg1	toolbox					1584:1590	the glyco-engineering toolbox	1562:1590	the glyco-engineering toolbox for CHO cells	1562:1604	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	1	59	theme	Recombinant	135:145	arg1	CHO					170:172	CHO	170:172	CHO	170:172	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	1	59	theme	Recombinant	135:145	arg1	ovary					163:167	Recombinant Chinese hamster ovary	135:167	Recombinant Chinese hamster ovary (CHO) cells	135:179	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	2	60	theme	plasma	447:452	arg1	members					381:387	two members	377:387	two members of the serpin superfamily	377:413	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	60	theme	plasma	447:452	arg1	C1INH					477:481	C1INH	477:481	C1INH	477:481	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	2	60	theme	plasma	447:452	arg1	inhibitor					466:474	plasma protease C1 inhibitor	447:474	plasma protease C1 inhibitor (C1INH)	447:482	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	8	61	dep	in	1486:1487	arg1	vitro					1489:1493	vitro	1489:1493	vitro	1489:1493	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	2	62	theme	C1INH	547:551	arg1	deficiency					553:562	C1INH deficiency	547:562	C1INH deficiency	547:562	Due to differences in N-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (A1AT) and plasma protease C1 inhibitor (C1INH), are currently derived from human plasma for treating A1AT and C1INH deficiency.					
30513349	9	63	theme	N-glycan	1671:1678	arg1	profiles					1680:1687	fully humanized N-glycan profiles	1655:1687	fully humanized N-glycan profiles	1655:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	1	64	theme	Chinese	147:153	arg1	CHO					170:172	CHO	170:172	CHO	170:172	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	1	64	theme	Chinese	147:153	arg1	ovary					163:167	Recombinant Chinese hamster ovary	135:167	Recombinant Chinese hamster ovary (CHO) cells	135:179	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	8	65	theme	enzymatic	1476:1484	arg1	activity					1495:1502	similar enzymatic in vitro activity	1468:1502	similar enzymatic in vitro activity	1468:1502	rhA1AT and rhC1INH produced in our glyco-engineered cell line showed no detectable differences to their plasma-purified counterparts on SDS-PAGE and had similar enzymatic in vitro activity.					
30513349	1	66	theme	humans	328:333	arg1	profiles					288:295	N-glycosylation profiles	272:295	N-glycosylation profiles similar, but not identical, to humans	272:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	10	67	link	plasma-derived	1712:1725	arg1	A1AT					1727:1730	plasma-derived A1AT	1712:1730	plasma-derived A1AT	1712:1730	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	1	68	theme	hamster	155:161	arg1	CHO					170:172	CHO	170:172	CHO	170:172	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	1	68	theme	hamster	155:161	arg1	ovary					163:167	Recombinant Chinese hamster ovary	135:167	Recombinant Chinese hamster ovary (CHO) cells	135:179	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	6	69	theme	gene	1042:1045	arg1	targets					1047:1053	10 gene targets	1039:1053	10 gene targets	1039:1053	This was achieved by combining CRISPR/Cas9-mediated disruption of 10 gene targets with overexpression of human ST6GAL1.					
30513349	9	70	theme	humanized	1661:1669	arg1	profiles					1680:1687	fully humanized N-glycan profiles	1655:1687	fully humanized N-glycan profiles	1655:1687	The work presented here shows the potential of expanding the glyco-engineering toolbox for CHO cells to produce a wider variety of glycoproteins with fully humanized N-glycan profiles.					
30513349	10	71	theme	plasma-derived	1712:1725	arg1	A1AT					1727:1730	plasma-derived A1AT	1712:1730	plasma-derived A1AT	1712:1730	We envision replacing plasma-derived A1AT and C1INH with recombinant versions and thereby decreasing our dependence on human donor blood, a limited and possibly unsafe protein source for patients.					
30513349	1	72	theme	ovary	163:167	arg1	cells					175:179	Recombinant Chinese hamster ovary (CHO) cells	135:179	Recombinant Chinese hamster ovary (CHO) cells	135:179	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	4	73	theme	produced	727:734	arg1	A1AT					736:739	Recombinantly produced A1AT	713:739	Recombinantly produced A1AT	713:739	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	4	73	theme	produced	727:734	arg1	rhA1AT					752:757	rhA1AT	752:757	rhA1AT	752:757	Recombinantly produced A1AT and C1INH (rhA1AT, rhC1INH) decorated with humanized N-glycans are therefore of clinical and commercial interest.					
30513349	6	74	theme	targets	1047:1053	arg1	disruption					1025:1034	CRISPR/Cas9-mediated disruption	1004:1034	CRISPR/Cas9-mediated disruption of 10 gene targets	1004:1053	This was achieved by combining CRISPR/Cas9-mediated disruption of 10 gene targets with overexpression of human ST6GAL1.					
30513349	1	75	contain	have	267:270	arg2	profiles					288:295	N-glycosylation profiles	272:295	N-glycosylation profiles similar, but not identical, to humans	272:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	1	75	contain	have	267:270	arg1	biopharmaceuticals					201:218	biopharmaceuticals	201:218	biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans	201:333	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	5	76	theme	engineered	872:881	arg1	lines					892:896	engineered CHO cell lines	872:896	engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles	872:970	Here, we present engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles.					
30513349	7	77	theme	rhC1INH	1163:1169	arg1	homogeneous					1175:1185	homogeneous	1175:1185	homogeneous	1175:1185	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	7	77	theme	rhC1INH	1163:1169	arg1	glyco-structures					1132:1147	the N-linked glyco-structures	1119:1147	the N-linked glyco-structures of rhA1AT and rhC1INH	1119:1169	We were able to show that the N-linked glyco-structures of rhA1AT and rhC1INH are homogeneous and similar to the structures obtained from plasma-derived A1AT and C1INH, marketed as Prolastin®-C and Cinryze®, respectively.					
30513349	1	78	theme	human	249:253	arg1	viruses					255:261	human viruses	249:261	human viruses	249:261	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
30513349	0	79	theme	esterase	69:76	arg1	inhibitor					78:86	alpha-1-antitrypsin and C1 esterase inhibitor	42:86	inhibitor	78:86	Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles.					
30513349	3	80	theme	transmitting	678:689	arg1	particles					702:710	transmitting infectious particles	678:710	transmitting infectious particles	678:710	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	3	81	theme	human	600:604	arg1	plasma					606:611	human plasma	600:611	human plasma	600:611	Deriving therapeutic proteins from human plasma is generally a cost-intensive process and also harbors a risk of transmitting infectious particles.					
30513349	5	82	theme	CHO	883:885	arg1	lines					892:896	engineered CHO cell lines	872:896	engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles	872:970	Here, we present engineered CHO cell lines producing rhA1AT or rhC1INH with fully humanized N-glycosylation profiles.					
30513349	1	83	theme	viruses	255:261	arg1	free					241:244	free	241:244	free	241:244	Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans.					
29444271	1	0	theme	ase	291:293	arg1	Sl-1					295:298	α-Fuc'ase Sl-1	285:298	α-Fuc'ase Sl-1	285:298	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	1	0	theme	ase	291:293	arg1	3/4-fucosidase					269:282	3/4-fucosidase	269:282	3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980)	269:341	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	2	1	theme	type	600:603	arg1	N-glycans					605:613	the complex type N-glycans	588:613	the complex type N-glycans containing Lea epitope(s)	588:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	1	2	dep	α1	265:266	arg1	lycopersicum					251:262	Solanum lycopersicum	243:262	Solanum lycopersicum	243:262	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	3	3	used	used	692:695	arg2	system					681:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	0	4	theme	α1,3-fucosyl	144:155	arg1	residue					157:163	the core α1,3-fucosyl residue	135:163	the core α1,3-fucosyl residue in plant N-glycans	135:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	5	theme	non-reducing	931:942	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	5	theme	non-reducing	931:942	arg1	residue					1091:1097	3-fucose residue	1082:1097	3-fucose residue	1082:1097	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	5	theme	non-reducing	931:942	arg1	residue					966:972	3-fucose residue	957:972	3-fucose residue on LNFP III and α1	957:991	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	5	theme	non-reducing	931:942	arg1	residues					1003:1010	4-fucose residues	994:1010	4-fucose residues of Lea epitopes on plant complex type N-glycans	994:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	5	theme	non-reducing	931:942	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	5	theme	non-reducing	931:942	arg1	α1					1078:1079	the core α1	1069:1079	the core α1	1069:1079	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	3	6	theme	anti-FLAG	733:741	arg1	tag					743:745	anti-FLAG tag	733:745	anti-FLAG tag	733:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	4	7	theme	type	1045:1048	arg1	N-glycans					1050:1058	plant complex type N-glycans	1031:1058	plant complex type N-glycans	1031:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	5	8	theme	Fucα1-3	1273:1279	arg1	GlcNAc					1281:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	4	9	theme	3-fucose	1082:1089	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	9	theme	3-fucose	1082:1089	arg1	residue					1091:1097	3-fucose residue	1082:1097	3-fucose residue	1082:1097	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	0	10	theme	plant	168:172	arg1	N-glycans					174:182	plant N-glycans	168:182	plant N-glycans	168:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	1	11	theme	ase	366:368	arg1	Sl-1					370:373	α-Fuc'ase Sl-1	360:373	α-Fuc'ase Sl-1	360:373	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	4	12	theme	terminal	944:951	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	12	theme	terminal	944:951	arg1	residue					1091:1097	3-fucose residue	1082:1097	3-fucose residue	1082:1097	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	12	theme	terminal	944:951	arg1	residue					966:972	3-fucose residue	957:972	3-fucose residue on LNFP III and α1	957:991	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	12	theme	terminal	944:951	arg1	residues					1003:1010	4-fucose residues	994:1010	4-fucose residues of Lea epitopes on plant complex type N-glycans	994:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	12	theme	terminal	944:951	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	12	theme	terminal	944:951	arg1	α1					1078:1079	the core α1	1069:1079	the core α1	1069:1079	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	1	13	theme	tomato	235:240	arg1	3/4-fucosidase					269:282	3/4-fucosidase	269:282	3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980)	269:341	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	1	13	theme	tomato	235:240	arg1	α1					265:266	a tomato (Solanum lycopersicum) α1	233:266	a tomato (Solanum lycopersicum) α1	233:266	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	3	14	theme	65	807:808	arg1	kDa					810:812	kDa	810:812	kDa	810:812	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	2	15	theme	ase	549:551	arg1	Sl-2					553:556	α-Fuc'ase Sl-2	543:556	α-Fuc'ase Sl-2	543:556	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	2	15	theme	ase	549:551	arg1	3/4-fucosidase					527:540	3/4-fucosidase	527:540	3/4-fucosidase (α-Fuc'ase Sl-2)	527:557	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	4	16	from	residues	1003:1010	arg1	N-glycans					1050:1058	plant complex type N-glycans	1031:1058	plant complex type N-glycans	1031:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	17	theme	ase	907:909	arg1	Sl-2					911:914	rFuc'ase Sl-2	902:914	rFuc'ase Sl-2	902:914	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	3	18	theme	expression	756:765	arg1	product					767:773	the expression product	752:773	the expression product (rFuc'ase Sl-2)	752:789	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	18	theme	expression	756:765	arg1	Sl-2					723:726	α-Fuc'ase Sl-2	713:726	α-Fuc'ase Sl-2 with anti-FLAG tag	713:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	18	theme	expression	756:765	arg1	protein					814:820	a 65 kDa protein	805:820	a 65 kDa protein using SDS-PAGE	805:835	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	18	theme	expression	756:765	arg1	Sl-2					785:788	rFuc'ase Sl-2	776:788	rFuc'ase Sl-2	776:788	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	2	19	contain	containing	615:624	arg1	N-glycans					605:613	the complex type N-glycans	588:613	the complex type N-glycans containing Lea epitope(s)	588:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	2	19	contain	containing	615:624	arg2	s					638:638	Lea epitope(s)	626:639	Lea epitope(s)	626:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	4	20	theme	core	1073:1076	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	20	theme	core	1073:1076	arg1	α1					1078:1079	the core α1	1069:1079	the core α1	1069:1079	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	2	21	theme	tomato	485:490	arg1	Solyc11g069010					498:511	Solyc11g069010	498:511	Solyc11g069010	498:511	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	2	21	theme	tomato	485:490	arg1	gene					492:495	another tomato gene	477:495	another tomato gene (Solyc11g069010)	477:512	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	5	22	theme	α1	1238:1239	arg1	residue					1251:1257	α1, 3-fucose residue	1238:1257	α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc	1238:1286	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	23	dep	α1	1238:1239	arg1	3-fucose					1242:1249	3-fucose	1242:1249	3-fucose	1242:1249	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	3	24	with	Sl-2	723:726	arg1	tag					743:745	anti-FLAG tag	733:745	anti-FLAG tag	733:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	25	theme	optimum	848:854	arg1	pH					856:857	an optimum pH	845:857	an optimum pH of around 5.0	845:871	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	5	26	theme	GlcNAc	1357:1362	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	26	theme	GlcNAc	1357:1362	arg1	residue					1364:1370	the proximal GlcNAc residue	1344:1370	the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1344:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	27	theme	plant	1409:1413	arg1	N-glycans					1424:1432	plant specific N-glycans	1409:1432	plant specific N-glycans	1409:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	28	theme	proximal	1348:1355	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	28	theme	proximal	1348:1355	arg1	residue					1364:1370	the proximal GlcNAc residue	1344:1370	the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1344:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	1	29	theme	tomato	314:319	arg1	Solyc03g006980					327:340	Solyc03g006980	327:340	Solyc03g006980	327:340	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	1	29	theme	tomato	314:319	arg1	gene					321:324	a tomato gene	312:324	a tomato gene (Solyc03g006980)	312:341	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	2	30	theme	epitope	630:636	arg1	s					638:638	Lea epitope(s)	626:639	Lea epitope(s)	626:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	5	31	theme	specific	1415:1422	arg1	N-glycans					1424:1432	plant specific N-glycans	1409:1432	plant specific N-glycans	1409:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	0	32	theme	Molecular	0:8	arg1	member					82:87	a member	80:87	a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	80:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	32	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	0:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	33	from	N-glycans	1050:1058	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	33	from	N-glycans	1050:1058	arg1	residues					1003:1010	4-fucose residues	994:1010	4-fucose residues of Lea epitopes on plant complex type N-glycans	994:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	33	from	N-glycans	1050:1058	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	6	34	theme	sites	1524:1528	arg1	structure					1497:1505	A 3 D modelled structure	1482:1505	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2	1482:1546	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	4	35	from	epitopes	1019:1026	arg1	N-glycans					1050:1058	plant complex type N-glycans	1031:1058	plant complex type N-glycans	1031:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	3	36	theme	ase	781:783	arg1	product					767:773	the expression product	752:773	the expression product (rFuc'ase Sl-2)	752:789	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	36	theme	ase	781:783	arg1	Sl-2					785:788	rFuc'ase Sl-2	776:788	rFuc'ase Sl-2	776:788	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	5	37	theme	N-glycans	1424:1432	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	0	38	theme	second	30:35	arg1	Sl-2					73:76	α-Fuc'ase Sl-2	63:76	α-Fuc'ase Sl-2	63:76	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	38	theme	second	30:35	arg1	α1,3/4-fucosidase					44:60	second tomato α1,3/4-fucosidase	30:60	second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	30:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	39	theme	hydrolase	101:109	arg1	family					111:116	glycosyl hydrolase family 29	92:119	glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	92:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	40	theme	Manβ1-4GlcNAcβ1-4	1102:1118	arg1	GlcNAc					1128:1133	Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc	1102:1133	Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc	1102:1133	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	41	theme	ase	892:894	arg1	Sl-1					896:899	rFuc'ase Sl-1	887:899	rFuc'ase Sl-1	887:899	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	0	42	from	residue	157:163	arg1	N-glycans					174:182	plant N-glycans	168:182	plant N-glycans	168:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	43	theme	glycosyl	92:99	arg1	family					111:116	glycosyl hydrolase family 29	92:119	glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	92:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	44	theme	plant	1031:1035	arg1	N-glycans					1050:1058	plant complex type N-glycans	1031:1058	plant complex type N-glycans	1031:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	5	45	theme	core	1379:1382	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	0	46	theme	α1,3/4-fucosidase	44:60	arg1	member					82:87	a member	80:87	a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	80:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	46	theme	α1,3/4-fucosidase	44:60	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	0:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	6	47	theme	D	1486:1486	arg1	structure					1497:1505	A 3 D modelled structure	1482:1505	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2	1482:1546	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	5	48	theme	GlcNAcβ1-4	1262:1271	arg1	GlcNAc					1281:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	4	49	theme	complex	1037:1043	arg1	N-glycans					1050:1058	plant complex type N-glycans	1031:1058	plant complex type N-glycans	1031:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	5	50	theme	tri-saccharide	1384:1397	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	0	51	theme	tomato	37:42	arg1	Sl-2					73:76	α-Fuc'ase Sl-2	63:76	α-Fuc'ase Sl-2	63:76	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	51	theme	tomato	37:42	arg1	α1,3/4-fucosidase					44:60	second tomato α1,3/4-fucosidase	30:60	second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	30:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	3	52	theme	baculovirus-insect	646:663	arg1	system					681:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	0	53	theme	family	111:116	arg1	member					82:87	a member	80:87	a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	80:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	53	theme	family	111:116	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	0:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	54	theme	4-fucose	994:1001	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	54	theme	4-fucose	994:1001	arg1	residues					1003:1010	4-fucose residues	994:1010	4-fucose residues of Lea epitopes on plant complex type N-glycans	994:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	54	theme	4-fucose	994:1001	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	55	from	residue	966:972	arg1	LNFP					977:980	LNFP III and α1	977:991	LNFP	977:980	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	55	from	residue	966:972	arg1	α1					990:991	α1	990:991	α1	990:991	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	2	56	theme	complex	592:598	arg1	N-glycans					605:613	the complex type N-glycans	588:613	the complex type N-glycans containing Lea epitope(s)	588:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	5	57	theme	moiety	1399:1404	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	57	theme	moiety	1399:1404	arg1	residue					1364:1370	the proximal GlcNAc residue	1344:1370	the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1344:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	3	58	theme	cell	665:668	arg1	system					681:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	1	59	theme	Lea	406:408	arg1	N-glycans					429:437	Lea epitope-containing N-glycans	406:437	Lea epitope-containing N-glycans	406:437	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	6	60	theme	fucose	1625:1630	arg1	residue					1632:1638	the α1, 3/4 fucose residue	1613:1638	residue	1632:1638	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	5	61	dep	ases	1187:1190	arg1	Sl-1					1192:1195	Sl-1	1192:1195	Sl-1	1192:1195	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	61	dep	ases	1187:1190	arg1	ases					1187:1190	both α-Fuc'ases Sl-1 and Sl-2	1176:1204	both α-Fuc'ases Sl-1 and Sl-2	1176:1204	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	61	dep	ases	1187:1190	arg1	Sl-2					1201:1204	Sl-2	1201:1204	Sl-2	1201:1204	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	4	62	theme	Fucα1-3	1120:1126	arg1	GlcNAc					1128:1133	Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc	1102:1133	Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc	1102:1133	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	0	63	theme	active	121:126	arg1	family					111:116	glycosyl hydrolase family 29	92:119	glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans	92:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	64	theme	epitopes	1019:1026	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	64	theme	epitopes	1019:1026	arg1	residues					1003:1010	4-fucose residues	994:1010	4-fucose residues of Lea epitopes on plant complex type N-glycans	994:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	64	theme	epitopes	1019:1026	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	1	65	theme	epitope-containing	410:427	arg1	N-glycans					429:437	Lea epitope-containing N-glycans	406:437	Lea epitope-containing N-glycans	406:437	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	6	66	theme	α1	1617:1618	arg1	residue					1632:1638	the α1, 3/4 fucose residue	1613:1638	residue	1632:1638	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	6	67	theme	modelled	1488:1495	arg1	structure					1497:1505	A 3 D modelled structure	1482:1505	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2	1482:1546	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	0	68	theme	core	139:142	arg1	residue					157:163	the core α1,3-fucosyl residue	135:163	the core α1,3-fucosyl residue in plant N-glycans	135:182	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	4	69	theme	Lea	1015:1017	arg1	epitopes					1019:1026	Lea epitopes	1015:1026	Lea epitopes on plant complex type N-glycans	1015:1058	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	1	70	theme	N-glycans	429:437	arg1	turnover					394:401	the turnover	390:401	the turnover of Lea epitope-containing N-glycans	390:437	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	1	71	theme	previous	190:197	arg1	study					199:203	a previous study	188:203	a previous study	188:203	In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans.					
29444271	3	72	with	product	767:773	arg1	tag					743:745	anti-FLAG tag	733:745	anti-FLAG tag	733:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	4	73	theme	3-fucose	957:964	arg1	α1					953:954	the non-reducing terminal α1	927:954	the non-reducing terminal α1	927:954	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	4	73	theme	3-fucose	957:964	arg1	residue					966:972	3-fucose residue	957:972	3-fucose residue on LNFP III and α1	957:991	Similarly to rFuc'ase Sl-1, rFuc'ase Sl-2 hydrolyzed the non-reducing terminal α1, 3-fucose residue on LNFP III and α1, 4-fucose residues of Lea epitopes on plant complex type N-glycans, but not the core α1, 3-fucose residue on Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc or Fucα1-3GlcNAc.					
29444271	2	74	theme	Lea	626:628	arg1	s					638:638	Lea epitope(s)	626:639	Lea epitope(s)	626:639	In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s).					
29444271	5	75	theme	ase	1468:1470	arg1	activity					1472:1479	α-Fuc'ase activity	1462:1479	α-Fuc'ase activity	1462:1479	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	6	76	theme	ase	1539:1541	arg1	Sl-2					1543:1546	α-Fuc'ase Sl-2	1533:1546	α-Fuc'ase Sl-2	1533:1546	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	0	77	theme	ase	69:71	arg1	Sl-2					73:76	α-Fuc'ase Sl-2	63:76	α-Fuc'ase Sl-2	63:76	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	0	77	theme	ase	69:71	arg1	α1,3/4-fucosidase					44:60	second tomato α1,3/4-fucosidase	30:60	second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2)	30:77	Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.					
29444271	3	78	theme	kDa	810:812	arg1	product					767:773	the expression product	752:773	the expression product (rFuc'ase Sl-2)	752:789	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	78	theme	kDa	810:812	arg1	Sl-2					723:726	α-Fuc'ase Sl-2	713:726	α-Fuc'ase Sl-2 with anti-FLAG tag	713:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	78	theme	kDa	810:812	arg1	protein					814:820	a 65 kDa protein	805:820	a 65 kDa protein using SDS-PAGE	805:835	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	79	theme	ase	719:721	arg1	Sl-2					723:726	α-Fuc'ase Sl-2	713:726	α-Fuc'ase Sl-2 with anti-FLAG tag	713:745	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	79	theme	ase	719:721	arg1	product					767:773	the expression product	752:773	the expression product (rFuc'ase Sl-2)	752:789	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	79	theme	ase	719:721	arg1	protein					814:820	a 65 kDa protein	805:820	a 65 kDa protein using SDS-PAGE	805:835	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	3	80	theme	expression	670:679	arg1	system					681:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system	642:686	The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.0.					
29444271	5	81	from	residue	1251:1257	arg1	GlcNAc					1281:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	GlcNAcβ1-4(Fucα1-3)GlcNAc	1262:1286	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	6	82	theme	catalytic	1514:1522	arg1	sites					1524:1528	the catalytic sites	1510:1528	the catalytic sites of α-Fuc'ase Sl-2	1510:1546	A 3 D modelled structure of the catalytic sites of α-Fuc'ase Sl-2 suggested that Asp192 and Glu236 may be important for binding to the α1, 3/4 fucose residue.					
29444271	5	83	theme	non-substituted	1309:1323	arg1	β-GlcNAc					1325:1332	the non-substituted β-GlcNAc	1305:1332	the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1305:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	83	theme	non-substituted	1309:1323	arg1	pre-requisite					1444:1456	a pre-requisite	1442:1456	a pre-requisite for α-Fuc'ase activity	1442:1479	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	84	attach	linked	1334:1339	arg2	β-GlcNAc					1325:1332	the non-substituted β-GlcNAc	1305:1332	the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1305:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	84	attach	linked	1334:1339	arg1	moiety					1399:1404	the core tri-saccharide moiety	1375:1404	the core tri-saccharide moiety of plant specific N-glycans	1375:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	84	attach	linked	1334:1339	arg1	residue					1364:1370	the proximal GlcNAc residue	1344:1370	the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans	1344:1432	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29444271	5	84	attach	linked	1334:1339	arg2	pre-requisite					1444:1456	a pre-requisite	1442:1456	a pre-requisite for α-Fuc'ase activity	1442:1479	However, we found that both α-Fuc'ases Sl-1 and Sl-2 were specifically active toward α1, 3-fucose residue on GlcNAcβ1-4(Fucα1-3)GlcNAc, indicating that the non-substituted β-GlcNAc linked to the proximal GlcNAc residue of the core tri-saccharide moiety of plant specific N-glycans must be a pre-requisite for α-Fuc'ase activity.					
29535710	9	0	theme	decreased	1545:1553	arg1	galactosylation					1555:1569	decreased galactosylation	1545:1569	decreased galactosylation	1545:1569	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	3	1	theme	IgG	451:453	arg1	glycosylation					455:467	IgG glycosylation	451:467	IgG glycosylation as well as corresponding subclass specificities	451:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	1	2	theme	wide	224:227	arg1	range					229:233	a wide range	222:233	a wide range of diseases	222:245	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	3	3	theme	subclass	494:501	arg1	specificities					503:515	corresponding subclass specificities	480:515	IgG glycosylation as well as corresponding subclass specificities	451:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	11	4	theme	IgG	2041:2043	arg1	glycosylation					2045:2057	IgG glycosylation	2041:2057	IgG glycosylation	2041:2057	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	2	5	theme	binding	272:278	arg1	region					280:285	the Fc binding region	265:285	the Fc binding region of IgGs, responsible for the antibody's effector function,	265:344	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	11	6	theme	immune	1978:1983	arg1	response					1985:1992	the immune response	1974:1992	the immune response	1974:1992	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	9	7	from	locus	1407:1411	arg1	chromosome					1416:1425	chromosome 1	1416:1427	chromosome 1	1416:1427	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	5	8	theme	IgG	886:888	arg1	glycans					890:896	IgG glycans	886:896	IgG glycans	886:896	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	1	9	gly	glycoprotein	100:111	arg1	G					89:89	Immunoglobulin G	74:89	Immunoglobulin G (IgG)	74:95	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	1	9	gly	glycoprotein	100:111	arg1	glycoprotein					100:111	a glycoprotein	98:111	a glycoprotein secreted by plasma B-cells	98:138	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	10	10	theme	class	1669:1673	arg1	switching					1675:1683	IgA class switching	1665:1683	IgA class switching	1665:1683	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	1	11	theme	adaptive	173:180	arg1	response					189:196	the human adaptive immune response	163:196	the human adaptive immune response	163:196	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	4	12	theme	association	601:611	arg1	GWAS					620:623	GWAS	620:623	GWAS	620:623	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	12	theme	association	601:611	arg1	study					613:617	a genome-wide association study	587:617	a genome-wide association study (GWAS)	587:624	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	11	13	theme	variants	1864:1871	arg1	impact					1854:1859	the impact	1850:1859	the impact of variants within RUNX3	1850:1884	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	4	14	theme	spectrometry	669:680	arg1	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	5	15	theme	glycosylation	984:996	arg1	pathways					998:1005	the glycosylation pathways	980:1005	the glycosylation pathways	980:1005	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	8	16	theme	glycosylation	1375:1387	arg1	patterns					1389:1396	the IgG glycosylation patterns	1367:1396	the IgG glycosylation patterns	1367:1396	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	10	17	theme	IgA	1665:1667	arg1	switching					1675:1683	IgA class switching	1665:1683	IgA class switching	1665:1683	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	4	18	from	Research	768:775	arg1	F4					806:807	F4	806:807	F4	806:807	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	18	from	Research	768:775	arg1	cohort					816:821	the Augsburg Region (KORA F4) study cohort	780:821	the Augsburg Region (KORA F4) study cohort	780:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	18	from	Research	768:775	arg1	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	1	19	theme	plasma	125:130	arg1	B-cells					132:138	plasma B-cells	125:138	plasma B-cells	125:138	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	4	20	theme	liquid	629:634	arg1	spectrometry					669:680	liquid chromatography electrospray mass spectrometry	629:680	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	20	theme	liquid	629:634	arg1	LC-ESI-MS					683:691	LC-ESI-MS	683:691	LC-ESI-MS	683:691	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	10	21	theme	T-cell	1718:1723	arg1	differentiation					1725:1739	T-cell differentiation	1718:1739	T-cell differentiation	1718:1739	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	0	22	from	Study	24:28	arg1	Patterns					64:71	Immunoglobulin G Glycosylation Patterns	33:71	Immunoglobulin G Glycosylation Patterns	33:71	Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns.					
29535710	11	23	theme	T-cell	1944:1949	arg1	differentiation					1951:1965	T-cell differentiation	1944:1965	T-cell differentiation	1944:1965	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	4	24	theme	electrospray	651:662	arg1	spectrometry					669:680	liquid chromatography electrospray mass spectrometry	629:680	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	24	theme	electrospray	651:662	arg1	LC-ESI-MS					683:691	LC-ESI-MS	683:691	LC-ESI-MS	683:691	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	7	25	theme	IgG	1163:1165	arg1	structures					1174:1183	single IgG glycan structures	1156:1183	single IgG glycan structures	1156:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	11	26	theme	B-cell	1922:1927	arg1	activation					1929:1938	B-cell activation	1922:1938	B-cell activation	1922:1938	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	1	27	theme	Immunoglobulin	74:87	arg1	G					89:89	Immunoglobulin G	74:89	Immunoglobulin G (IgG)	74:95	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	1	27	theme	Immunoglobulin	74:87	arg1	glycoprotein					100:111	a glycoprotein	98:111	a glycoprotein secreted by plasma B-cells	98:138	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	1	27	theme	Immunoglobulin	74:87	arg1	IgG					92:94	IgG	92:94	IgG	92:94	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	4	28	gly	glycopeptides	707:719	arg1	individuals					730:740	1,823 individuals	724:740	1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	724:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	28	gly	glycopeptides	707:719	arg2	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	29	from	glycopeptides	707:719	arg1	Research					768:775	the Cooperative Health Research	745:775	the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	745:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	8	30	contain	have	1341:1344	arg1	loci					1336:1339	other genetic loci	1322:1339	other genetic loci	1322:1339	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	8	30	contain	have	1341:1344	arg2	influences					1353:1362	strong influences	1346:1362	strong influences	1346:1362	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	4	31	theme	-measured	693:701	arg1	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	0	32	theme	Genome-Wide	0:10	arg1	Association					12:22	Genome-Wide Association	0:22	Genome-Wide Association	0:22	Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns.					
29535710	1	33	theme	diseases	238:245	arg1	range					229:233	a wide range	222:233	a wide range of diseases	222:245	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	5	34	from	GWAS	850:853	arg1	ratios					876:881	subclass-specific ratios	858:881	subclass-specific ratios of IgG glycans	858:896	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	9	35	theme	transcription	1467:1479	arg1	factor					1481:1486	a transcription factor	1465:1486	a transcription factor of the runt domain-containing family	1465:1523	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	2	36	theme	immune	382:387	arg1	response					389:396	a proper immune response	373:396	a proper immune response	373:396	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	11	37	from	involvement	1768:1778	arg1	glycosylation					1811:1823	IgG glycosylation	1807:1823	IgG glycosylation	1807:1823	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	0	38	theme	Immunoglobulin	33:46	arg1	Patterns					64:71	Immunoglobulin G Glycosylation Patterns	33:71	Immunoglobulin G Glycosylation Patterns	33:71	Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns.					
29535710	9	39	theme	runt	1495:1498	arg1	family					1518:1523	the runt domain-containing family	1491:1523	the runt domain-containing family	1491:1523	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	4	40	theme	Region	793:798	arg1	F4					806:807	F4	806:807	F4	806:807	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	40	theme	Region	793:798	arg1	cohort					816:821	the Augsburg Region (KORA F4) study cohort	780:821	the Augsburg Region (KORA F4) study cohort	780:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	3	41	theme	genetic	433:439	arg1	impact					441:446	the general genetic impact	421:446	the general genetic impact on IgG glycosylation as well as corresponding subclass specificities	421:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	0	42	theme	Glycosylation	50:62	arg1	Patterns					64:71	Immunoglobulin G Glycosylation Patterns	33:71	Immunoglobulin G Glycosylation Patterns	33:71	Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns.					
29535710	9	43	theme	family	1518:1523	arg1	factor					1481:1486	a transcription factor	1465:1486	a transcription factor of the runt domain-containing family	1465:1523	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	4	44	theme	Cooperative	749:759	arg1	Research					768:775	the Cooperative Health Research	745:775	the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	745:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	6	45	from	Study	1082:1086	arg1	individuals					1044:1054	1,836 individuals	1038:1054	1,836 individuals from the Leiden Longevity Study (LLS)	1038:1092	We replicated our findings in 1,836 individuals from the Leiden Longevity Study (LLS).					
29535710	2	46	theme	IgGs	290:293	arg1	region					280:285	the Fc binding region	265:285	the Fc binding region of IgGs, responsible for the antibody's effector function,	265:344	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	8	47	dep	genes	1239:1243	arg1	addition					1227:1234	addition	1227:1234	addition	1227:1234	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	6	48	theme	Leiden	1065:1070	arg1	LLS					1089:1091	LLS	1089:1091	LLS	1089:1091	We replicated our findings in 1,836 individuals from the Leiden Longevity Study (LLS).					
29535710	6	48	theme	Leiden	1065:1070	arg1	Study					1082:1086	the Leiden Longevity Study	1061:1086	the Leiden Longevity Study (LLS)	1061:1092	We replicated our findings in 1,836 individuals from the Leiden Longevity Study (LLS).					
29535710	4	49	theme	IgG	555:557	arg1	glycosylation					559:571	IgG glycosylation	555:571	IgG glycosylation	555:571	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	8	50	theme	strong	1346:1351	arg1	influences					1353:1362	strong influences	1346:1362	strong influences	1346:1362	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	5	51	theme	single	954:959	arg1	steps					971:975	single enzymatic steps	954:975	single enzymatic steps in the glycosylation pathways	954:1005	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	4	52	theme	study	810:814	arg1	F4					806:807	F4	806:807	F4	806:807	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	52	theme	study	810:814	arg1	cohort					816:821	the Augsburg Region (KORA F4) study cohort	780:821	the Augsburg Region (KORA F4) study cohort	780:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	53	theme	genetic	530:536	arg1	loci					538:541	genetic loci	530:541	genetic loci involved in IgG glycosylation	530:571	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	2	54	gly	Glycosylation	248:260	arg1	region					280:285	the Fc binding region	265:285	the Fc binding region of IgGs, responsible for the antibody's effector function,	265:344	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	8	55	theme	other	1322:1326	arg1	loci					1336:1339	other genetic loci	1322:1339	other genetic loci	1322:1339	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	3	56	theme	corresponding	480:492	arg1	specificities					503:515	corresponding subclass specificities	480:515	IgG glycosylation as well as corresponding subclass specificities	451:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	10	57	theme	B-cell	1689:1694	arg1	maturation					1696:1705	B-cell maturation	1689:1705	B-cell maturation	1689:1705	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	2	58	theme	responsible	296:306	arg1	IgGs					290:293	IgGs	290:293	IgGs	290:293	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	1	59	theme	major	149:153	arg1	role					155:158	a major role	147:158	a major role	147:158	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	2	60	theme	region	280:285	arg1	Glycosylation					248:260	Glycosylation	248:260	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function,	248:344	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	8	61	theme	replicated	1190:1199	arg1	results					1201:1207	The replicated results	1186:1207	The replicated results	1186:1207	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	3	62	from	impact	441:446	arg1	glycosylation					455:467	IgG glycosylation	451:467	IgG glycosylation as well as corresponding subclass specificities	451:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	3	62	from	impact	441:446	arg1	specificities					503:515	corresponding subclass specificities	480:515	IgG glycosylation as well as corresponding subclass specificities	451:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	2	63	theme	Fc	269:270	arg1	region					280:285	the Fc binding region	265:285	the Fc binding region of IgGs, responsible for the antibody's effector function,	265:344	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	1	64	theme	human	167:171	arg1	response					189:196	the human adaptive immune response	163:196	the human adaptive immune response	163:196	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	5	65	from	steps	971:975	arg1	pathways					998:1005	the glycosylation pathways	980:1005	the glycosylation pathways	980:1005	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	1	66	theme	immune	182:187	arg1	response					189:196	the human adaptive immune response	163:196	the human adaptive immune response	163:196	Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases.					
29535710	8	67	theme	IgG	1371:1373	arg1	patterns					1389:1396	the IgG glycosylation patterns	1367:1396	the IgG glycosylation patterns	1367:1396	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	4	68	theme	genome-wide	589:599	arg1	GWAS					620:623	GWAS	620:623	GWAS	620:623	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	68	theme	genome-wide	589:599	arg1	study					613:617	a genome-wide association study	587:617	a genome-wide association study (GWAS)	587:624	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	9	69	theme	novel	1401:1405	arg1	locus					1407:1411	A novel locus	1399:1411	A novel locus	1399:1411	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	11	70	theme	cell	2005:2008	arg1	migration					2010:2018	cell migration	2005:2018	cell migration	2005:2018	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	4	71	theme	mass	664:667	arg1	spectrometry					669:680	liquid chromatography electrospray mass spectrometry	629:680	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	71	theme	mass	664:667	arg1	LC-ESI-MS					683:691	LC-ESI-MS	683:691	LC-ESI-MS	683:691	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	5	72	theme	subclass-specific	858:874	arg1	ratios					876:881	subclass-specific ratios	858:881	subclass-specific ratios of IgG glycans	858:896	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	10	73	theme	family	1607:1612	arg1	members					1587:1593	members	1587:1593	members of the RUNX family	1587:1612	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	7	74	theme	glycan	1167:1172	arg1	structures					1174:1183	single IgG glycan structures	1156:1183	single IgG glycan structures	1156:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	7	75	theme	single	1156:1161	arg1	structures					1174:1183	single IgG glycan structures	1156:1183	single IgG glycan structures	1156:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	11	76	theme	glycosyltransferases	1783:1802	arg1	involvement					1768:1778	the involvement	1764:1778	the involvement of glycosyltransferases in IgG glycosylation	1764:1823	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
29535710	4	77	theme	chromatography	636:649	arg1	spectrometry					669:680	liquid chromatography electrospray mass spectrometry	629:680	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	77	theme	chromatography	636:649	arg1	LC-ESI-MS					683:691	LC-ESI-MS	683:691	LC-ESI-MS	683:691	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	78	from	individuals	730:740	arg1	Research					768:775	the Cooperative Health Research	745:775	the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	745:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	10	79	theme	RUNX	1602:1605	arg1	family					1607:1612	the RUNX family	1598:1612	the RUNX family	1598:1612	Interestingly, members of the RUNX family are cross-regulated, and RUNX3 is involved in both IgA class switching and B-cell maturation as well as T-cell differentiation and apoptosis.					
29535710	7	80	theme	subclass-specific	1116:1132	arg1	influences					1142:1151	subclass-specific genetic influences	1116:1151	subclass-specific genetic influences on single IgG glycan structures	1116:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	4	81	theme	individuals	730:740	arg1	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	2	82	theme	effector	327:334	arg1	function					336:343	the antibody's effector function	312:343	the antibody's effector function	312:343	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	5	83	theme	glycans	890:896	arg1	ratios					876:881	subclass-specific ratios	858:881	subclass-specific ratios of IgG glycans	858:896	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	2	84	theme	proper	375:380	arg1	response					389:396	a proper immune response	373:396	a proper immune response	373:396	Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response.					
29535710	4	85	theme	IgG	703:705	arg1	glycopeptides					707:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides	629:719	liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	629:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	0	86	theme	G	48:48	arg1	Patterns					64:71	Immunoglobulin G Glycosylation Patterns	33:71	Immunoglobulin G Glycosylation Patterns	33:71	Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns.					
29535710	8	87	dep	ST6GAL1	1286:1292	arg1	i.e.					1280:1283	i.e.	1280:1283	i.e.	1280:1283	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	5	88	theme	enzymatic	961:969	arg1	steps					971:975	single enzymatic steps	954:975	single enzymatic steps in the glycosylation pathways	954:1005	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	7	89	theme	genetic	1134:1140	arg1	influences					1142:1151	subclass-specific genetic influences	1116:1151	subclass-specific genetic influences on single IgG glycan structures	1116:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	9	90	theme	domain-containing	1500:1516	arg1	family					1518:1523	the runt domain-containing family	1491:1523	the runt domain-containing family	1491:1523	A novel locus on chromosome 1, harboring RUNX3, which encodes for a transcription factor of the runt domain-containing family, is associated with decreased galactosylation.					
29535710	4	91	theme	Augsburg	784:791	arg1	F4					806:807	F4	806:807	F4	806:807	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	4	91	theme	Augsburg	784:791	arg1	cohort					816:821	the Augsburg Region (KORA F4) study cohort	780:821	the Augsburg Region (KORA F4) study cohort	780:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	7	92	from	influences	1142:1151	arg1	structures					1174:1183	single IgG glycan structures	1156:1183	single IgG glycan structures	1156:1183	We were able to show subclass-specific genetic influences on single IgG glycan structures.					
29535710	5	93	theme	genetic	927:933	arg1	factors					935:941	genetic factors	927:941	genetic factors underlying single enzymatic steps in the glycosylation pathways	927:1005	In addition, we performed GWAS on subclass-specific ratios of IgG glycans to gain power in identifying genetic factors underlying single enzymatic steps in the glycosylation pathways.					
29535710	6	94	theme	Longevity	1072:1080	arg1	LLS					1089:1091	LLS	1089:1091	LLS	1089:1091	We replicated our findings in 1,836 individuals from the Leiden Longevity Study (LLS).					
29535710	6	94	theme	Longevity	1072:1080	arg1	Study					1082:1086	the Leiden Longevity Study	1061:1086	the Leiden Longevity Study (LLS)	1061:1092	We replicated our findings in 1,836 individuals from the Leiden Longevity Study (LLS).					
29535710	8	95	theme	genetic	1328:1334	arg1	loci					1336:1339	other genetic loci	1322:1339	other genetic loci	1322:1339	The replicated results indicate that, in addition to genes encoding for glycosyltransferases (i.e., ST6GAL1, B4GALT1, FUT8, and MGAT3), other genetic loci have strong influences on the IgG glycosylation patterns.					
29535710	3	96	theme	general	425:431	arg1	impact					441:446	the general genetic impact	421:446	the general genetic impact on IgG glycosylation as well as corresponding subclass specificities	421:515	This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities.					
29535710	4	97	theme	Health	761:766	arg1	Research					768:775	the Cooperative Health Research	745:775	the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort	745:821	To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort.					
29535710	11	98	theme	IgG	1807:1809	arg1	glycosylation					1811:1823	IgG glycosylation	1807:1823	IgG glycosylation	1807:1823	Besides the involvement of glycosyltransferases in IgG glycosylation, we suggest that, due to the impact of variants within RUNX3, potentially mechanisms involved in B-cell activation and T-cell differentiation during the immune response as well as cell migration and invasion involve IgG glycosylation.					
30835642	6	0	theme	serum	920:924	arg1	composition					905:915	The IgG N-glycan composition	888:915	The IgG N-glycan composition of serum	888:924	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	3	1	theme	cellular	521:528	arg1	processes					530:538	posttranslational cellular processes	503:538	posttranslational cellular processes	503:538	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	9	2	theme	body	1308:1311	arg1	index					1318:1322	body mass index	1308:1322	body mass index	1308:1322	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	9	3	from	subjects	1386:1393	arg1	individuals					1350:1360	individuals	1350:1360	individuals with hyperuricemia from subjects without hyperuricemia	1350:1415	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	2	4	theme	renal	362:366	arg1	damage					368:373	renal damage	362:373	renal damage	362:373	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	9	5	theme	gender	1329:1334	arg1	combination					1282:1292	The combination	1278:1292	The combination of GP9, GP10, body mass index, and gender	1278:1334	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	7	6	from	%	1063:1063	arg1	women					1084:1088	women	1084:1088	women	1084:1088	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	7	6	from	%	1063:1063	arg1	men					1068:1070	men	1068:1070	men	1068:1070	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	0	7	theme	Biomarkers	101:110	arg1	Study					85:89	A Community-Based Study	67:89	Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers	33:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	11	8	theme	diagnostics	1805:1815	arg1	development					1783:1793	the development	1779:1793	the development of glycan diagnostics	1779:1815	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	8	9	dep	correlated	1129:1138	arg1	whereas					1196:1202	whereas	1196:1202	whereas	1196:1202	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	9	10	theme	0.849	1456:1460	arg1	value					1447:1451	the curve value	1437:1451	the curve value of 0.849 (95% confidence interval: 0.784-0.915)	1437:1499	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	4	11	from	hyperuricemia	700:712	arg1	population					729:738	the general population	717:738	the general population	717:738	We evaluated whether immunoglobulin (Ig)G N-glycans play a role in hyperuricemia in the general population.					
30835642	0	12	theme	Glycan	94:99	arg1	Biomarkers					101:110	Glycan Biomarkers	94:110	Glycan Biomarkers	94:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	6	13	theme	N-glycan	896:903	arg1	composition					905:915	The IgG N-glycan composition	888:915	The IgG N-glycan composition of serum	888:924	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	11	14	from	hyperuricemia	1821:1833	arg1	future					1851:1856	future	1851:1856	future	1851:1856	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	4	15	theme	general	721:727	arg1	population					729:738	the general population	717:738	the general population	717:738	We evaluated whether immunoglobulin (Ig)G N-glycans play a role in hyperuricemia in the general population.					
30835642	9	16	theme	GP9	1297:1299	arg1	combination					1282:1292	The combination	1278:1292	The combination of GP9, GP10, body mass index, and gender	1278:1334	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	7	17	dep	%	1056:1056	arg1	%					1079:1079	1.6%	1076:1079	1.6% in women	1076:1088	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	7	17	dep	%	1056:1056	arg1	%					1063:1063	14.9%	1059:1063	14.9% in men	1059:1070	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	6	18	theme	IgG	892:894	arg1	composition					905:915	The IgG N-glycan composition	888:915	The IgG N-glycan composition of serum	888:924	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP					1159:1160	GP	1159:1160	GP	1159:1160	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP4					1170:1172	GP4	1170:1172	GP4	1170:1172	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP6					1175:1177	GP6	1175:1177	GP6	1175:1177	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP2					1165:1167	GP2	1165:1167	GP2	1165:1167	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP10					1180:1183	GP10	1180:1183	GP10	1180:1183	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	peaks					1152:1156	glycan peaks	1145:1156	glycan peaks (GP)1	1145:1162	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	8	19	theme	glycan	1145:1150	arg1	GP11					1190:1193	GP11	1190:1193	GP11	1190:1193	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	1	20	theme	public	191:196	arg1	prevalence					128:137	The increasing prevalence	113:137	The increasing prevalence of hyperuricemia	113:154	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	20	theme	public	191:196	arg1	concern					205:211	an emerging public health concern	179:211	an emerging public health concern in both developed and developing countries	179:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	3	21	theme	genetic	452:458	arg1	variation					460:468	human genetic variation	446:468	human genetic variation	446:468	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	3	21	theme	genetic	452:458	arg1	factor					495:500	a factor	493:500	a factor	493:500	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	7	22	located	observed	1024:1031	arg2	hyperuricemia					1010:1022	hyperuricemia	1010:1022	hyperuricemia observed in this sample	1010:1046	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	7	22	located	observed	1024:1031	arg1	sample					1041:1046	this sample	1036:1046	this sample	1036:1046	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	10	23	theme	possible	1540:1547	arg1	link					1549:1552	a possible link	1538:1552	a possible link	1538:1552	These findings collectively suggest a possible link between hyperuricemia and IgG N-glycans, which might be potentially mediated through inflammation-related mechanisms.					
30835642	1	24	theme	emerging	182:189	arg1	prevalence					128:137	The increasing prevalence	113:137	The increasing prevalence of hyperuricemia	113:154	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	24	theme	emerging	182:189	arg1	concern					205:211	an emerging public health concern	179:211	an emerging public health concern in both developed and developing countries	179:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	5	25	theme	ages	819:822	arg1	participants					781:792	635 participants	777:792	635 participants (208 men and 427 women)	777:816	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	5	25	theme	ages	819:822	arg1	years					828:832	ages ≥18 years	819:832	ages ≥18 years	819:832	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	3	26	theme	glycan	544:549	arg1	biomarkers					551:560	glycan biomarkers	544:560	glycan biomarkers	544:560	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	11	27	theme	independent	1716:1726	arg1	samples					1759:1765	independent and community-based population samples	1716:1765	independent and community-based population samples	1716:1765	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	8	28	theme	acid	1103:1106	arg1	level					1108:1112	Serum uric acid level	1092:1112	Serum uric acid level	1092:1112	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	9	29	with	individuals	1350:1360	arg1	hyperuricemia					1367:1379	hyperuricemia	1367:1379	hyperuricemia	1367:1379	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	2	30	theme	autoimmune	386:395	arg1	disorders					397:405	autoimmune disorders	386:405	autoimmune disorders	386:405	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	11	31	theme	Additional	1672:1681	arg1	research					1683:1690	Additional research	1672:1690	Additional research on glycan biomarkers in independent and community-based population samples	1672:1765	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	11	32	from	research	1683:1690	arg1	biomarkers					1702:1711	glycan biomarkers	1695:1711	glycan biomarkers	1695:1711	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	11	32	from	research	1683:1690	arg1	samples					1759:1765	independent and community-based population samples	1716:1765	independent and community-based population samples	1716:1765	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	2	33	theme	uric	331:334	arg1	acid					336:339	an elevated serum uric acid	313:339	an elevated serum uric acid	313:339	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	1	34	theme	health	198:203	arg1	prevalence					128:137	The increasing prevalence	113:137	The increasing prevalence of hyperuricemia	113:154	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	34	theme	health	198:203	arg1	concern					205:211	an emerging public health concern	179:211	an emerging public health concern in both developed and developing countries	179:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	2	35	theme	serum	325:329	arg1	acid					336:339	an elevated serum uric acid	313:339	an elevated serum uric acid	313:339	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	9	36	theme	mass	1313:1316	arg1	index					1318:1322	body mass index	1308:1322	body mass index	1308:1322	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	6	37	theme	chromatography	969:982	arg1	method					984:989	an ultraperformance liquid chromatography method	942:989	an ultraperformance liquid chromatography method	942:989	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	2	38	theme	elevated	316:323	arg1	acid					336:339	an elevated serum uric acid	313:339	an elevated serum uric acid	313:339	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	9	39	theme	index	1318:1322	arg1	combination					1282:1292	The combination	1278:1292	The combination of GP9, GP10, body mass index, and gender	1278:1334	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	9	40	dep	%	1465:1465	arg1	0.784-0.915					1488:1498	0.784-0.915	1488:1498	0.784-0.915	1488:1498	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	6	41	theme	liquid	962:967	arg1	method					984:989	an ultraperformance liquid chromatography method	942:989	an ultraperformance liquid chromatography method	942:989	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	11	42	theme	community-based	1732:1746	arg1	samples					1759:1765	independent and community-based population samples	1716:1765	independent and community-based population samples	1716:1765	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	5	43	theme	community-based	842:856	arg1	population					858:867	a community-based population	840:867	a community-based population in Beijing, China	840:885	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	3	44	theme	human	446:450	arg1	variation					460:468	human genetic variation	446:468	human genetic variation	446:468	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	3	44	theme	human	446:450	arg1	factor					495:500	a factor	493:500	a factor	493:500	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	2	45	theme	cardiovascular	412:425	arg1	diseases					427:434	cardiovascular diseases	412:434	cardiovascular diseases	412:434	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	0	46	theme	Immunoglobulin	33:46	arg1	G					48:48	Immunoglobulin G	33:48	Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers	33:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	6	47	theme	ultraperformance	945:960	arg1	method					984:989	an ultraperformance liquid chromatography method	942:989	an ultraperformance liquid chromatography method	942:989	The IgG N-glycan composition of serum was analyzed by an ultraperformance liquid chromatography method.					
30835642	5	48	theme	cross-sectional	746:760	arg1	study					762:766	This cross-sectional study	741:766	This cross-sectional study	741:766	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	10	49	theme	inflammation-related	1639:1658	arg1	mechanisms					1660:1669	inflammation-related mechanisms	1639:1669	inflammation-related mechanisms	1639:1669	These findings collectively suggest a possible link between hyperuricemia and IgG N-glycans, which might be potentially mediated through inflammation-related mechanisms.					
30835642	1	50	theme	developed	221:229	arg1	countries					246:254	both developed and developing countries	216:254	both developed and developing countries	216:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	7	51	from	%	1079:1079	arg1	women					1084:1088	women	1084:1088	women	1084:1088	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	7	51	from	%	1079:1079	arg1	men					1068:1070	men	1068:1070	men	1068:1070	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	0	52	dep	G	48:48	arg1	Study					85:89	A Community-Based Study	67:89	Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers	33:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	0	52	dep	G	48:48	arg1	N-Glycosylation					50:64	N-Glycosylation	50:64	Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers	33:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	5	53	from	population	858:867	arg1	China					881:885	China	881:885	China	881:885	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	1	54	theme	increasing	117:126	arg1	prevalence					128:137	The increasing prevalence	113:137	The increasing prevalence of hyperuricemia	113:154	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	54	theme	increasing	117:126	arg1	concern					205:211	an emerging public health concern	179:211	an emerging public health concern in both developed and developing countries	179:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	10	55	theme	IgG	1580:1582	arg1	N-glycans					1584:1592	IgG N-glycans	1580:1592	IgG N-glycans	1580:1592	These findings collectively suggest a possible link between hyperuricemia and IgG N-glycans, which might be potentially mediated through inflammation-related mechanisms.					
30835642	5	56	dep	participants	781:792	arg1	men					799:801	208 men	795:801	208 men	795:801	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	5	56	dep	participants	781:792	arg1	women					811:815	427 women	807:815	427 women	807:815	This cross-sectional study enrolled 635 participants (208 men and 427 women), ages ≥18 years, from a community-based population in Beijing, China.					
30835642	1	57	from	concern	205:211	arg1	countries					246:254	both developed and developing countries	216:254	both developed and developing countries	216:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	3	58	theme	posttranslational	503:519	arg1	processes					530:538	posttranslational cellular processes	503:538	posttranslational cellular processes	503:538	Although human genetic variation has been recognized as a factor, posttranslational cellular processes and glycan biomarkers have not been studied extensively for susceptibility to hyperuricemia.					
30835642	11	59	theme	population	1748:1757	arg1	samples					1759:1765	independent and community-based population samples	1716:1765	independent and community-based population samples	1716:1765	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	8	60	theme	Serum	1092:1096	arg1	level					1108:1112	Serum uric acid level	1092:1112	Serum uric acid level	1092:1112	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	2	61	theme	metabolic	276:284	arg1	condition					286:294	a metabolic condition	274:294	a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases	274:434	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	2	61	theme	metabolic	276:284	arg1	Hyperuricemia					257:269	Hyperuricemia	257:269	Hyperuricemia	257:269	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	11	62	from	gout	1839:1842	arg1	future					1851:1856	future	1851:1856	future	1851:1856	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	9	63	theme	curve	1441:1445	arg1	value					1447:1451	the curve value	1437:1451	the curve value of 0.849 (95% confidence interval: 0.784-0.915)	1437:1499	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	11	64	theme	glycan	1798:1803	arg1	diagnostics					1805:1815	glycan diagnostics	1798:1815	glycan diagnostics	1798:1815	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	8	65	dep	peaks	1152:1156	arg1	1					1162:1162	1	1162:1162	1	1162:1162	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	1	66	theme	hyperuricemia	142:154	arg1	prevalence					128:137	The increasing prevalence	113:137	The increasing prevalence of hyperuricemia	113:154	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	66	theme	hyperuricemia	142:154	arg1	concern					205:211	an emerging public health concern	179:211	an emerging public health concern in both developed and developing countries	179:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	1	67	theme	developing	235:244	arg1	countries					246:254	both developed and developing countries	216:254	both developed and developing countries	216:254	The increasing prevalence of hyperuricemia has been recognized as an emerging public health concern in both developed and developing countries.					
30835642	0	68	theme	Community-Based	69:83	arg1	Study					85:89	A Community-Based Study	67:89	Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers	33:110	Hyperuricemia is Associated with Immunoglobulin G N-Glycosylation: A Community-Based Study of Glycan Biomarkers.					
30835642	4	69	theme	G	673:673	arg1	N-glycans					675:683	immunoglobulin (Ig)G N-glycans	654:683	immunoglobulin (Ig)G N-glycans	654:683	We evaluated whether immunoglobulin (Ig)G N-glycans play a role in hyperuricemia in the general population.					
30835642	7	70	theme	hyperuricemia	1010:1022	arg1	%					1056:1056	5.98%	1052:1056	5.98%	1052:1056	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	7	70	theme	hyperuricemia	1010:1022	arg1	prevalence					996:1005	The prevalence	992:1005	The prevalence of hyperuricemia observed in this sample	992:1046	The prevalence of hyperuricemia observed in this sample was 5.98% (14.9% in men and 1.6% in women).					
30835642	9	71	theme	GP10	1302:1305	arg1	combination					1282:1292	The combination	1278:1292	The combination of GP9, GP10, body mass index, and gender	1278:1334	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	11	72	theme	glycan	1695:1700	arg1	biomarkers					1702:1711	glycan biomarkers	1695:1711	glycan biomarkers	1695:1711	Additional research on glycan biomarkers in independent and community-based population samples might allow the development of glycan diagnostics for hyperuricemia and gout in the future.					
30835642	8	73	theme	uric	1098:1101	arg1	level					1108:1112	Serum uric acid level	1092:1112	Serum uric acid level	1092:1112	Serum uric acid level was positively correlated with glycan peaks (GP)1, GP2, GP4, GP6, GP10, and GP11, whereas it was negatively correlated with GP12, GP13, GP14, GP15, GP18, and GP20.					
30835642	9	74	theme	interval	1478:1485	arg1	0.849					1456:1460	0.849	1456:1460	0.849	1456:1460	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	9	74	theme	interval	1478:1485	arg1	%					1465:1465	95%	1463:1465	95% confidence interval	1463:1485	The combination of GP9, GP10, body mass index, and gender distinguished individuals with hyperuricemia from subjects without hyperuricemia, with an area under the curve value of 0.849 (95% confidence interval: 0.784-0.915).					
30835642	2	75	dep	condition	286:294	arg1	associated					346:355	associated	346:355	associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases	346:434	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	2	75	dep	condition	286:294	arg1	characterized					296:308	characterized	296:308	characterized by an elevated serum uric acid	296:339	Hyperuricemia is a metabolic condition characterized by an elevated serum uric acid, and associated with renal damage, diabetes, autoimmune disorders, and cardiovascular diseases.					
30835642	4	76	theme	immunoglobulin	654:667	arg1	N-glycans					675:683	immunoglobulin (Ig)G N-glycans	654:683	immunoglobulin (Ig)G N-glycans	654:683	We evaluated whether immunoglobulin (Ig)G N-glycans play a role in hyperuricemia in the general population.					
29655002	5	0	theme	fibrinogen	644:653	arg1	chains					655:660	the individual fibrinogen chains	629:660	the individual fibrinogen chains	629:660	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	10	1	theme	healthy	1579:1585	arg1	individuals					1587:1597	healthy individuals	1579:1597	healthy individuals	1579:1597	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	11	2	theme	deeper	1691:1696	arg1	understanding					1698:1710	deeper understanding	1691:1710	deeper understanding of coagulopathies	1691:1728	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	5	3	theme	work	467:470	arg1	aim					455:457	The aim	451:457	The aim of this work	451:470	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	4	theme	carbohydrate	883:894	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	4	theme	carbohydrate	883:894	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	4	theme	carbohydrate	883:894	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	4	theme	carbohydrate	883:894	arg1	Man					985:987	terminal α-2,3 Sia and α-1,3 Man	956:987	Man	985:987	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	4	5	theme	main	392:395	arg1	Fibrinogen					374:383	Fibrinogen	374:383	Fibrinogen	374:383	Fibrinogen is the main protein involved in clot formation and wound healing.					
29655002	4	5	theme	main	392:395	arg1	protein					397:403	the main protein	388:403	the main protein involved in clot formation and wound healing	388:448	Fibrinogen is the main protein involved in clot formation and wound healing.					
29655002	7	6	theme	Aα	1099:1100	arg1	susceptible					1121:1131	susceptible	1121:1131	susceptible	1121:1131	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	7	6	theme	Aα	1099:1100	arg1	chain					1102:1106	Fibrinogen Aα chain	1088:1106	Fibrinogen Aα chain	1088:1106	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	0	7	theme	Structural	0:9	arg1	changes					11:17	Structural changes	0:17	Structural changes of fibrinogen	0:31	Structural changes of fibrinogen as a consequence of cirrhosis.					
29655002	3	8	theme	Portal	217:222	arg1	hypertension					224:235	Portal hypertension	217:235	Portal hypertension	217:235	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	5	9	with	patients	812:819	arg1	cirrhosis					826:834	cirrhosis	826:834	cirrhosis	826:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	8	10	theme	α	1243:1243	arg1	chain					1245:1249	the α chain	1239:1249	the α chain	1239:1249	Cirrhosis induced additional protein carbonylation, mostly on the α chain.					
29655002	11	11	from	molecules	1640:1648	arg1	process					1665:1671	coagulation process	1653:1671	coagulation process	1653:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	5	12	theme	secondary	697:705	arg1	structure					720:728	the molecular secondary and tertiary structure	683:728	the molecular secondary and tertiary structure	683:728	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	8	13	theme	protein	1206:1212	arg1	carbonylation					1214:1226	additional protein carbonylation	1195:1226	additional protein carbonylation	1195:1226	Cirrhosis induced additional protein carbonylation, mostly on the α chain.					
29655002	6	14	theme	terminal	956:963	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	14	theme	terminal	956:963	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	15	with	reactivity	734:743	arg1	protein					789:795	the insulin-like growth factor-binding protein 1	750:797	the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	750:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	15	with	reactivity	734:743	arg1	IGFBP-1					800:806	IGFBP-1	800:806	IGFBP-1	800:806	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	16	theme	tertiary	711:718	arg1	structure					720:728	the molecular secondary and tertiary structure	683:728	the molecular secondary and tertiary structure	683:728	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	9	17	theme	protein	1334:1340	arg1	unfolding					1342:1350	protein unfolding	1334:1350	protein unfolding	1334:1350	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	5	18	with	structure	720:728	arg1	protein					789:795	the insulin-like growth factor-binding protein 1	750:797	the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	750:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	18	with	structure	720:728	arg1	IGFBP-1					800:806	IGFBP-1	800:806	IGFBP-1	800:806	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	19	theme	fibrinogen	529:538	arg1	molecules					540:548	the isolated fibrinogen molecules	516:548	the isolated fibrinogen molecules	516:548	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	20	theme	Gal	938:940	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	20	theme	Gal	938:940	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	11	21	from	information	1777:1787	arg1	mechanisms					1805:1814	the possible mechanisms	1792:1814	the possible mechanisms involved in impaired coagulation due to cirrhosis	1792:1864	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	3	22	theme	bleeding	318:325	arg1	disorders					327:335	bleeding disorders	318:335	bleeding disorders	318:335	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	4	23	theme	clot	417:420	arg1	formation					422:430	clot formation	417:430	clot formation	417:430	Fibrinogen is the main protein involved in clot formation and wound healing.					
29655002	5	24	theme	insulin-like	754:765	arg1	protein					789:795	the insulin-like growth factor-binding protein 1	750:797	the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	750:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	24	theme	insulin-like	754:765	arg1	IGFBP-1					800:806	IGFBP-1	800:806	IGFBP-1	800:806	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	9	25	theme	CD	1275:1276	arg1	spectrometry					1278:1289	CD spectrometry	1275:1289	CD spectrometry	1275:1289	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	1	26	theme	various	125:131	arg1	conditions					133:142	various conditions	125:142	various conditions	125:142	Cirrhosis is a disease which may develop as a consequence of various conditions.					
29655002	6	27	from	decrease	996:1003	arg1	Fuc					1019:1021	core α-1,6 Fuc	1008:1021	core α-1,6 Fuc	1008:1021	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	27	from	decrease	996:1003	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	27	from	decrease	996:1003	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	27	from	decrease	996:1003	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	27	from	decrease	996:1003	arg1	N-glycans					1055:1063	bi-antennary galactosylated N-glycans	1027:1063	bi-antennary galactosylated N-glycans	1027:1063	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	27	from	decrease	996:1003	arg1	Man					985:987	terminal α-2,3 Sia and α-1,3 Man	956:987	Man	985:987	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	28	theme	factor-binding	774:787	arg1	protein					789:795	the insulin-like growth factor-binding protein 1	750:797	the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	750:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	28	theme	factor-binding	774:787	arg1	IGFBP-1					800:806	IGFBP-1	800:806	IGFBP-1	800:806	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	29	from	protein	789:795	arg1	patients					812:819	patients	812:819	patients with cirrhosis	812:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	30	theme	α-1,3	979:983	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	30	theme	α-1,3	979:983	arg1	Man					985:987	terminal α-2,3 Sia and α-1,3 Man	956:987	Man	985:987	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	7	31	dep	susceptible	1121:1131	arg1	followed					1151:1158	followed	1151:1158	followed by the Bβ chain	1151:1174	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	9	32	theme	α-helix	1317:1323	arg1	content					1325:1331	the α-helix content	1313:1331	the α-helix content	1313:1331	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	7	33	theme	Bβ	1167:1168	arg1	chain					1170:1174	the Bβ chain	1163:1174	the Bβ chain	1163:1174	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	5	34	theme	protein	566:572	arg1	microarray					574:583	lectin-based protein microarray	553:583	lectin-based protein microarray	553:583	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	35	theme	carbonylation	604:616	arg1	pattern					618:624	the carbonylation pattern	600:624	the carbonylation pattern of the individual fibrinogen chains	600:660	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	36	theme	galactosylated	1040:1053	arg1	N-glycans					1055:1063	bi-antennary galactosylated N-glycans	1027:1063	bi-antennary galactosylated N-glycans	1027:1063	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	10	37	theme	wound	1504:1508	arg1	healing					1510:1516	wound healing	1504:1516	wound healing	1504:1516	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	6	38	with	N-glycans	1055:1063	arg1	GlcNAc					1080:1085	bisecting GlcNAc	1070:1085	bisecting GlcNAc	1070:1085	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	0	39	theme	cirrhosis	53:61	arg1	consequence					38:48	a consequence	36:48	a consequence of cirrhosis	36:61	Structural changes of fibrinogen as a consequence of cirrhosis.					
29655002	2	40	theme	liver	157:161	arg1	disease					163:169	advanced liver disease	148:169	advanced liver disease	148:169	In advanced liver disease, blood coagulation can be seriously affected.					
29655002	6	41	theme	α-1,6	1013:1017	arg1	Fuc					1019:1021	core α-1,6 Fuc	1008:1021	core α-1,6 Fuc	1008:1021	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	11	42	from	process	1665:1671	arg1	knowledge					1616:1624	A more detailed knowledge	1600:1624	A more detailed knowledge of individual molecules in coagulation process	1600:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	11	43	theme	coagulopathies	1715:1728	arg1	understanding					1698:1710	deeper understanding	1691:1710	deeper understanding of coagulopathies	1691:1728	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	11	44	theme	detailed	1607:1614	arg1	knowledge					1616:1624	A more detailed knowledge	1600:1624	A more detailed knowledge of individual molecules in coagulation process	1600:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	9	45	theme	modified	1373:1380	arg1	residues					1393:1400	modified amino acid residues	1373:1400	modified amino acid residues in cirrhosis	1373:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	9	46	from	cirrhosis	1405:1413	arg1	appearance					1359:1368	appearance	1359:1368	appearance of modified amino acid residues in cirrhosis	1359:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	6	47	with	Fuc	1019:1021	arg1	GlcNAc					1080:1085	bisecting GlcNAc	1070:1085	bisecting GlcNAc	1070:1085	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	11	48	theme	study	1754:1758	arg1	results					1738:1744	the results	1734:1744	the results of this study	1734:1758	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	9	49	theme	acid	1388:1391	arg1	residues					1393:1400	modified amino acid residues	1373:1400	modified amino acid residues in cirrhosis	1373:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	9	50	from	residues	1393:1400	arg1	cirrhosis					1405:1413	cirrhosis	1405:1413	cirrhosis	1405:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	11	51	theme	molecules	1640:1648	arg1	knowledge					1616:1624	A more detailed knowledge	1600:1624	A more detailed knowledge of individual molecules in coagulation process	1600:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	5	52	theme	individual	633:642	arg1	chains					655:660	the individual fibrinogen chains	629:660	the individual fibrinogen chains	629:660	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	53	theme	tri/tetra-antennary	906:924	arg1	structures					926:935	tri/tetra-antennary structures	906:935	tri/tetra-antennary structures	906:935	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	10	54	with	patients	1547:1554	arg1	cirrhosis					1561:1569	cirrhosis	1561:1569	cirrhosis	1561:1569	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	11	55	theme	coagulation	1653:1663	arg1	process					1665:1671	coagulation process	1653:1671	coagulation process	1653:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	5	56	theme	chains	655:660	arg1	pattern					618:624	the carbonylation pattern	600:624	the carbonylation pattern of the individual fibrinogen chains	600:660	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	7	57	theme	Fibrinogen	1088:1097	arg1	susceptible					1121:1131	susceptible	1121:1131	susceptible	1121:1131	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	7	57	theme	Fibrinogen	1088:1097	arg1	chain					1102:1106	Fibrinogen Aα chain	1088:1106	Fibrinogen Aα chain	1088:1106	Fibrinogen Aα chain was the most susceptible to carbonylation, followed by the Bβ chain.					
29655002	11	58	from	knowledge	1616:1624	arg1	process					1665:1671	coagulation process	1653:1671	coagulation process	1653:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	6	59	theme	several	875:881	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	59	theme	several	875:881	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	59	theme	several	875:881	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	59	theme	several	875:881	arg1	Man					985:987	terminal α-2,3 Sia and α-1,3 Man	956:987	Man	985:987	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	3	60	theme	vascular	238:245	arg1	abnormalities					247:259	vascular abnormalities	238:259	vascular abnormalities	238:259	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	6	61	from	increase	863:870	arg1	Fuc					1019:1021	core α-1,6 Fuc	1008:1021	core α-1,6 Fuc	1008:1021	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	61	from	increase	863:870	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	61	from	increase	863:870	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	61	from	increase	863:870	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	61	from	increase	863:870	arg1	N-glycans					1055:1063	bi-antennary galactosylated N-glycans	1027:1063	bi-antennary galactosylated N-glycans	1027:1063	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	61	from	increase	863:870	arg1	Man					985:987	terminal α-2,3 Sia and α-1,3 Man	956:987	Man	985:987	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	62	theme	molecular	687:695	arg1	structure					720:728	the molecular secondary and tertiary structure	683:728	the molecular secondary and tertiary structure	683:728	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	63	theme	α-2,3	965:969	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	63	theme	α-2,3	965:969	arg1	Sia					971:973	terminal α-2,3 Sia and α-1,3 Man	956:987	Sia	971:973	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	4	64	theme	wound	436:440	arg1	healing					442:448	wound healing	436:448	wound healing	436:448	Fibrinogen is the main protein involved in clot formation and wound healing.					
29655002	11	65	theme	impaired	1828:1835	arg1	coagulation					1837:1847	impaired coagulation	1828:1847	impaired coagulation due to cirrhosis	1828:1864	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	9	66	from	reduction	1300:1308	arg1	content					1325:1331	the α-helix content	1313:1331	the α-helix content	1313:1331	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	5	67	theme	possible	663:670	arg1	changes					672:678	possible changes	663:678	possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	663:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	68	theme	isolated	520:527	arg1	molecules					540:548	the isolated fibrinogen molecules	516:548	the isolated fibrinogen molecules	516:548	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	69	theme	β-1,4	942:946	arg1	GlcNAc					948:953	Gal β-1,4 GlcNAc	938:953	Gal β-1,4 GlcNAc	938:953	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	6	69	theme	β-1,4	942:946	arg1	moieties					896:903	several carbohydrate moieties	875:903	several carbohydrate moieties	875:903	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	70	theme	molecules	540:548	arg1	pattern					505:511	the glycosylation pattern	487:511	the glycosylation pattern of the isolated fibrinogen molecules	487:548	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	6	71	theme	core	1008:1011	arg1	Fuc					1019:1021	core α-1,6 Fuc	1008:1021	core α-1,6 Fuc	1008:1021	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	3	72	theme	coagulation	284:294	arg1	factors					296:302	coagulation factors	284:302	coagulation factors	284:302	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	5	73	theme	glycosylation	491:503	arg1	pattern					505:511	the glycosylation pattern	487:511	the glycosylation pattern of the isolated fibrinogen molecules	487:548	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	11	74	theme	additional	1766:1775	arg1	information					1777:1787	additional information	1766:1787	additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis	1766:1864	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	5	75	theme	growth	767:772	arg1	protein					789:795	the insulin-like growth factor-binding protein 1	750:797	the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	750:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	75	theme	growth	767:772	arg1	IGFBP-1					800:806	IGFBP-1	800:806	IGFBP-1	800:806	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	1	76	theme	conditions	133:142	arg1	consequence					110:120	a consequence	108:120	a consequence of various conditions	108:142	Cirrhosis is a disease which may develop as a consequence of various conditions.					
29655002	9	77	from	appearance	1359:1368	arg1	cirrhosis					1405:1413	cirrhosis	1405:1413	cirrhosis	1405:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	10	78	theme	complexes	1430:1438	arg1	complexes					1430:1438	complexes	1430:1438	complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing	1430:1516	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	10	78	theme	complexes	1430:1438	arg1	amount					1420:1425	The amount	1416:1425	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing	1416:1516	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	10	78	theme	complexes	1430:1438	arg1	greater					1536:1542	greater	1536:1542	greater	1536:1542	The amount of complexes which fibrinogen forms with IGFBP-1, another factor involved in wound healing was significantly greater in patients with cirrhosis than in healthy individuals.					
29655002	11	79	theme	possible	1796:1803	arg1	mechanisms					1805:1814	the possible mechanisms	1792:1814	the possible mechanisms involved in impaired coagulation due to cirrhosis	1792:1864	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	6	80	theme	bisecting	1070:1078	arg1	GlcNAc					1080:1085	bisecting GlcNAc	1070:1085	bisecting GlcNAc	1070:1085	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	81	theme	lectin-based	553:564	arg1	microarray					574:583	lectin-based protein microarray	553:583	lectin-based protein microarray	553:583	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	0	82	theme	fibrinogen	22:31	arg1	changes					11:17	Structural changes	0:17	Structural changes of fibrinogen	0:31	Structural changes of fibrinogen as a consequence of cirrhosis.					
29655002	3	83	from	hypertension	224:235	arg1	factors					296:302	coagulation factors	284:302	coagulation factors	284:302	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	6	84	theme	bi-antennary	1027:1038	arg1	N-glycans					1055:1063	bi-antennary galactosylated N-glycans	1027:1063	bi-antennary galactosylated N-glycans	1027:1063	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	3	85	theme	thrombosis	362:371	arg1	development					347:357	the development	343:357	the development of thrombosis	343:371	Portal hypertension, vascular abnormalities and/or a dysbalance in coagulation factors may result in bleeding disorders or in the development of thrombosis.					
29655002	5	86	gly	glycosylation	491:503	arg1	molecules					540:548	the isolated fibrinogen molecules	516:548	the isolated fibrinogen molecules	516:548	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	8	87	theme	additional	1195:1204	arg1	carbonylation					1214:1226	additional protein carbonylation	1195:1226	additional protein carbonylation	1195:1226	Cirrhosis induced additional protein carbonylation, mostly on the α chain.					
29655002	2	88	theme	advanced	148:155	arg1	disease					163:169	advanced liver disease	148:169	advanced liver disease	148:169	In advanced liver disease, blood coagulation can be seriously affected.					
29655002	9	89	theme	amino	1382:1386	arg1	residues					1393:1400	modified amino acid residues	1373:1400	modified amino acid residues in cirrhosis	1373:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	2	90	theme	blood	172:176	arg1	coagulation					178:188	blood coagulation	172:188	blood coagulation	172:188	In advanced liver disease, blood coagulation can be seriously affected.					
29655002	11	91	theme	individual	1629:1638	arg1	molecules					1640:1648	individual molecules	1629:1648	individual molecules in coagulation process	1629:1671	A more detailed knowledge of individual molecules in coagulation process may contribute to deeper understanding of coagulopathies and the results of this study offer additional information on the possible mechanisms involved in impaired coagulation due to cirrhosis.					
29655002	9	92	theme	residues	1393:1400	arg1	appearance					1359:1368	appearance	1359:1368	appearance of modified amino acid residues in cirrhosis	1359:1413	Spectrofluorimetry and CD spectrometry detected reduction in the α-helix content, protein unfolding and/or appearance of modified amino acid residues in cirrhosis.					
29655002	0	93	dep	changes	11:17	arg1	consequence					38:48	a consequence	36:48	a consequence of cirrhosis	36:61	Structural changes of fibrinogen as a consequence of cirrhosis.					
29655002	6	94	dep	moieties	896:903	arg1	structures					926:935	tri/tetra-antennary structures	906:935	tri/tetra-antennary structures	906:935	The results pointed to an increase in several carbohydrate moieties: tri/tetra-antennary structures, Gal β-1,4 GlcNAc, terminal α-2,3 Sia and α-1,3 Man, and a decrease in core α-1,6 Fuc and bi-antennary galactosylated N-glycans with bisecting GlcNAc.					
29655002	5	95	from	changes	672:678	arg1	reactivity					734:743	reactivity	734:743	reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis	734:834	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
29655002	5	95	from	changes	672:678	arg1	structure					720:728	the molecular secondary and tertiary structure	683:728	the molecular secondary and tertiary structure	683:728	The aim of this work was to analyse the glycosylation pattern of the isolated fibrinogen molecules by lectin-based protein microarray, together with the carbonylation pattern of the individual fibrinogen chains, possible changes in the molecular secondary and tertiary structure and reactivity with the insulin-like growth factor-binding protein 1 (IGFBP-1) in patients with cirrhosis.					
31338503	6	0	from	lectins	990:996	arg1	comparison					953:962	a systematic comparison	940:962	a systematic comparison of microarray data from 8 lectins	940:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	0	from	lectins	990:996	arg1	data					978:981	microarray data	967:981	microarray data from 8 lectins	967:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	9	1	theme	microarray	1366:1375	arg1	data					1377:1380	microarray data	1366:1380	microarray data	1366:1380	The results provide insights for interpreting microarray data and designing future glycan microarrays.					
31338503	6	2	from	range	1007:1011	arg1	platforms					1068:1076	the CFG and neoglycoprotein array platforms	1034:1076	platforms	1068:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	2	from	range	1007:1011	arg1	CFG					1038:1040	the CFG and neoglycoprotein array platforms	1034:1076	CFG	1038:1040	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	3	3	theme	glycan	545:550	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	6	4	theme	microarray	967:976	arg1	data					978:981	microarray data	967:981	microarray data from 8 lectins	967:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	4	5	theme	many	632:635	arg1	interactions					658:669	many protein-carbohydrate interactions	632:669	many protein-carbohydrate interactions	632:669	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	3	6	theme	numerous	352:359	arg1	platforms					388:396	numerous distinct glycan microarray platforms	352:396	numerous distinct glycan microarray platforms	352:396	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	4	7	theme	Comparative	563:573	arg1	analyses					575:582	Comparative analyses	563:582	Comparative analyses of glycan microarray data	563:608	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	3	8	theme	densities	552:560	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	8	theme	densities	552:560	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	8	theme	densities	552:560	arg1	linkers					532:538	linkers	532:538	linkers	532:538	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	8	theme	densities	552:560	arg1	variety					441:447	a variety	439:447	a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities	439:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	8	theme	densities	552:560	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	8	theme	densities	552:560	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	6	9	theme	array	1062:1066	arg1	platforms					1068:1076	the CFG and neoglycoprotein array platforms	1034:1076	platforms	1068:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	10	from	comparison	953:962	arg1	lectins					990:996	8 lectins	988:996	8 lectins	988:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	11	theme	data	978:981	arg1	comparison					953:962	a systematic comparison	940:962	a systematic comparison of microarray data from 8 lectins	940:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	12	from	concentrations	1016:1029	arg1	platforms					1068:1076	the CFG and neoglycoprotein array platforms	1034:1076	platforms	1068:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	12	from	concentrations	1016:1029	arg1	CFG					1038:1040	the CFG and neoglycoprotein array platforms	1034:1076	CFG	1038:1040	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	13	theme	neoglycoprotein	1046:1060	arg1	platforms					1068:1076	the CFG and neoglycoprotein array platforms	1034:1076	platforms	1068:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	2	14	theme	Glycan	173:178	arg1	microarrays					180:190	Glycan microarrays	173:190	Glycan microarrays	173:190	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	4	15	theme	protein-carbohydrate	637:656	arg1	interactions					658:669	many protein-carbohydrate interactions	632:669	many protein-carbohydrate interactions	632:669	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	3	16	theme	microarray	377:386	arg1	platforms					388:396	numerous distinct glycan microarray platforms	352:396	numerous distinct glycan microarray platforms	352:396	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	9	17	theme	future	1396:1401	arg1	microarrays					1410:1420	future glycan microarrays	1396:1420	future glycan microarrays	1396:1420	The results provide insights for interpreting microarray data and designing future glycan microarrays.					
31338503	1	18	theme	Protein-carbohydrate	75:94	arg1	interactions					96:107	Protein-carbohydrate interactions	75:107	Protein-carbohydrate interactions	75:107	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	4	19	theme	various	735:741	arg1	formats					749:755	various array formats	735:755	various array formats	735:755	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	2	20	used	utilized	205:212	arg2	microarrays					180:190	Glycan microarrays	173:190	Glycan microarrays	173:190	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	9	21	theme	glycan	1403:1408	arg1	microarrays					1410:1420	future glycan microarrays	1396:1420	future glycan microarrays	1396:1420	The results provide insights for interpreting microarray data and designing future glycan microarrays.					
31338503	2	22	theme	protein-carbohydrate	288:307	arg1	interactions					309:320	these complex protein-carbohydrate interactions	274:320	these complex protein-carbohydrate interactions	274:320	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	3	23	theme	glycan-attachment	479:495	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	5	24	theme	variances	878:886	arg1	sources					867:873	sources	867:873	sources of variances across array platforms	867:909	For optimal construction and use of this technology, it is important to understand sources of variances across array platforms.					
31338503	6	25	theme	systematic	942:951	arg1	comparison					953:962	a systematic comparison	940:962	a systematic comparison of microarray data from 8 lectins	940:996	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	26	from	platforms	1068:1076	arg1	range					1007:1011	a range	1005:1011	a range of concentrations on the CFG and neoglycoprotein array platforms	1005:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	3	27	theme	slide-surface	452:464	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	7	28	theme	lectins	1152:1158	arg1	specificity					1133:1143	the binding specificity	1121:1143	the binding specificity of the lectins on the two arrays	1121:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	1	29	theme	significant	114:124	arg1	roles					126:130	significant roles	114:130	significant roles	114:130	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	3	30	theme	chemistries	466:476	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	30	theme	chemistries	466:476	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	30	theme	chemistries	466:476	arg1	linkers					532:538	linkers	532:538	linkers	532:538	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	30	theme	chemistries	466:476	arg1	variety					441:447	a variety	439:447	a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities	439:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	30	theme	chemistries	466:476	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	30	theme	chemistries	466:476	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	5	31	theme	array	895:899	arg1	platforms					901:909	array platforms	895:909	array platforms	895:909	For optimal construction and use of this technology, it is important to understand sources of variances across array platforms.					
31338503	4	32	theme	data	605:608	arg1	analyses					575:582	Comparative analyses	563:582	Comparative analyses of glycan microarray data	563:608	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	3	33	theme	glycan	510:515	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	7	34	theme	discrepancies	1210:1222	arg1	cases					1195:1199	some cases	1190:1199	some cases of large discrepancies	1190:1222	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	2	35	theme	interactions	309:320	arg1	sensitivity					246:256	sensitivity	246:256	sensitivity	246:256	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	2	35	theme	interactions	309:320	arg1	breadth					263:269	breadth	263:269	breadth	263:269	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	2	35	theme	interactions	309:320	arg1	selectivity					233:243	selectivity	233:243	selectivity	233:243	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	7	36	theme	general	1100:1106	arg1	agreement					1108:1116	good general agreement	1095:1116	good general agreement on the binding specificity of the lectins on the two arrays	1095:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	3	37	theme	presentations	517:529	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	37	theme	presentations	517:529	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	37	theme	presentations	517:529	arg1	linkers					532:538	linkers	532:538	linkers	532:538	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	37	theme	presentations	517:529	arg1	variety					441:447	a variety	439:447	a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities	439:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	37	theme	presentations	517:529	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	37	theme	presentations	517:529	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	0	38	theme	glycan	39:44	arg1	profiles					65:72	glycan microarray binding profiles	39:72	glycan microarray binding profiles	39:72	Factors contributing to variability of glycan microarray binding profiles.					
31338503	6	39	theme	concentrations	1016:1029	arg1	range					1007:1011	a range	1005:1011	a range of concentrations on the CFG and neoglycoprotein array platforms	1005:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	6	40	from	CFG	1038:1040	arg1	range					1007:1011	a range	1005:1011	a range of concentrations on the CFG and neoglycoprotein array platforms	1005:1076	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	8	41	theme	glycan	1240:1245	arg1	density					1247:1253	glycan density	1240:1253	glycan density	1240:1253	Differences in glycan density and linker composition contributed significantly to variability.					
31338503	4	42	theme	array	743:747	arg1	formats					749:755	various array formats	735:755	various array formats	735:755	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	3	43	theme	chemistries	497:507	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	43	theme	chemistries	497:507	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	43	theme	chemistries	497:507	arg1	linkers					532:538	linkers	532:538	linkers	532:538	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	43	theme	chemistries	497:507	arg1	variety					441:447	a variety	439:447	a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities	439:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	43	theme	chemistries	497:507	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	43	theme	chemistries	497:507	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	4	44	theme	different	765:773	arg1	results					775:781	different results	765:781	different results	765:781	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	7	45	theme	binding	1125:1131	arg1	specificity					1133:1143	the binding specificity	1121:1143	the binding specificity of the lectins on the two arrays	1121:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	7	46	from	specificity	1133:1143	arg1	arrays					1171:1176	the two arrays	1163:1176	the two arrays	1163:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	7	47	theme	large	1204:1208	arg1	discrepancies					1210:1222	large discrepancies	1204:1222	large discrepancies	1204:1222	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	5	48	theme	technology	825:834	arg1	use					813:815	use	813:815	use	813:815	For optimal construction and use of this technology, it is important to understand sources of variances across array platforms.					
31338503	5	48	theme	technology	825:834	arg1	construction					796:807	construction	796:807	construction	796:807	For optimal construction and use of this technology, it is important to understand sources of variances across array platforms.					
31338503	0	49	theme	binding	57:63	arg1	profiles					65:72	glycan microarray binding profiles	39:72	glycan microarray binding profiles	39:72	Factors contributing to variability of glycan microarray binding profiles.					
31338503	8	50	theme	linker	1259:1264	arg1	composition					1266:1276	linker composition	1259:1276	linker composition	1259:1276	Differences in glycan density and linker composition contributed significantly to variability.					
31338503	0	51	theme	profiles	65:72	arg1	variability					24:34	variability	24:34	variability of glycan microarray binding profiles	24:72	Factors contributing to variability of glycan microarray binding profiles.					
31338503	0	52	theme	microarray	46:55	arg1	profiles					65:72	glycan microarray binding profiles	39:72	glycan microarray binding profiles	39:72	Factors contributing to variability of glycan microarray binding profiles.					
31338503	4	53	theme	microarray	594:603	arg1	data					605:608	glycan microarray data	587:608	glycan microarray data	587:608	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	8	54	from	Differences	1225:1235	arg1	density					1247:1253	glycan density	1240:1253	glycan density	1240:1253	Differences in glycan density and linker composition contributed significantly to variability.					
31338503	8	54	from	Differences	1225:1235	arg1	composition					1266:1276	linker composition	1259:1276	linker composition	1259:1276	Differences in glycan density and linker composition contributed significantly to variability.					
31338503	1	55	theme	wide	137:140	arg1	variety					142:148	a wide variety	135:148	a wide variety of biological systems	135:170	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	1	55	theme	wide	137:140	arg1	systems					164:170	biological systems	153:170	biological systems	153:170	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	4	56	theme	glycan	587:592	arg1	data					605:608	glycan microarray data	587:608	glycan microarray data	587:608	Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results.					
31338503	7	57	theme	good	1095:1098	arg1	agreement					1108:1116	good general agreement	1095:1116	good general agreement on the binding specificity of the lectins on the two arrays	1095:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	2	58	theme	complex	280:286	arg1	interactions					309:320	these complex protein-carbohydrate interactions	274:320	these complex protein-carbohydrate interactions	274:320	Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions.					
31338503	6	59	gly	neoglycoprotein	1046:1060	arg1	neoglycoprotein					1046:1060	the CFG and neoglycoprotein array platforms	1034:1076	neoglycoprotein	1046:1060	In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms.					
31338503	3	60	theme	past	334:337	arg1	decades					343:349	the past two decades	330:349	the past two decades	330:349	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	densities					552:560	glycan densities	545:560	glycan densities	545:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	chemistries					466:476	slide-surface chemistries	452:476	slide-surface chemistries	452:476	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	linkers					532:538	linkers	532:538	linkers	532:538	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	variety					441:447	a variety	439:447	a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities	439:560	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	chemistries					497:507	glycan-attachment chemistries	479:507	glycan-attachment chemistries	479:507	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	3	61	theme	linkers	532:538	arg1	presentations					517:529	glycan presentations	510:529	glycan presentations	510:529	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	5	62	theme	optimal	788:794	arg1	construction					796:807	construction	796:807	construction	796:807	For optimal construction and use of this technology, it is important to understand sources of variances across array platforms.					
31338503	3	63	theme	distinct	361:368	arg1	platforms					388:396	numerous distinct glycan microarray platforms	352:396	numerous distinct glycan microarray platforms	352:396	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	1	64	theme	biological	153:162	arg1	systems					164:170	biological systems	153:170	biological systems	153:170	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	7	65	from	agreement	1108:1116	arg1	specificity					1133:1143	the binding specificity	1121:1143	the binding specificity of the lectins on the two arrays	1121:1176	While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies.					
31338503	3	66	theme	glycan	370:375	arg1	platforms					388:396	numerous distinct glycan microarray platforms	352:396	numerous distinct glycan microarray platforms	352:396	During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities.					
31338503	1	67	theme	systems	164:170	arg1	variety					142:148	a wide variety	135:148	a wide variety of biological systems	135:170	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31338503	1	67	theme	systems	164:170	arg1	systems					164:170	biological systems	153:170	biological systems	153:170	Protein-carbohydrate interactions play significant roles in a wide variety of biological systems.					
31478651	5	0	theme	tailspike	1046:1054	arg1	proteins					1056:1063	bacteriophage tailspike proteins	1032:1063	bacteriophage tailspike proteins	1032:1063	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion.					
31478651	3	1	from	dynamics	573:580	arg1	matrices					612:619	matrices	612:619	matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii	612:747	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	1	2	theme	polysaccharides	176:190	arg1	Biofilms					124:131	Biofilms	124:131	Biofilms	124:131	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	2	theme	polysaccharides	176:190	arg1	mixtures					145:152	complex mixtures	137:152	complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle	137:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	3	theme	bacterial	295:303	arg1	cycle					310:314	the bacterial life cycle	291:314	the bacterial life cycle	291:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	6	4	theme	diffusion	1250:1258	arg1	barrier					1260:1266	a selective diffusion barrier	1238:1266	a selective diffusion barrier for nanometer-sized objects	1238:1294	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	6	4	theme	diffusion	1250:1258	arg1	biofilm					1215:1221	the biofilm	1211:1221	the biofilm	1211:1221	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	2	5	theme	contributions	394:406	arg1	analysis					378:385	a precise analysis	368:385	a precise analysis of the contributions of individual matrix components to the biofilm structural organization	368:477	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	3	6	theme	nanometer-sized	585:599	arg1	objects					601:607	nanometer-sized objects	585:607	nanometer-sized objects	585:607	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	5	7	theme	bacteriophage	1032:1044	arg1	proteins					1056:1063	bacteriophage tailspike proteins	1032:1063	bacteriophage tailspike proteins	1032:1063	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion.					
31478651	2	8	theme	compositional	332:344	arg1	heterogeneity					346:358	their compositional heterogeneity	326:358	their compositional heterogeneity	326:358	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	1	9	theme	life	305:308	arg1	cycle					310:314	the bacterial life cycle	291:314	the bacterial life cycle	291:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	4	10	theme	notable	859:865	arg1	dynamics					880:887	notable subdiffusive dynamics	859:887	notable subdiffusive dynamics dependent on probe size and stewartan concentration	859:939	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	4	11	theme	dependent	889:897	arg1	dynamics					880:887	notable subdiffusive dynamics	859:887	notable subdiffusive dynamics dependent on probe size and stewartan concentration	859:939	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	6	12	theme	glycan	1135:1140	arg1	stewartan					1150:1158	the glycan polymer stewartan	1131:1158	the glycan polymer stewartan	1131:1158	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	4	13	theme	single-particle	792:806	arg1	tracking					808:815	single-particle tracking	792:815	single-particle tracking	792:815	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	6	14	theme	biofilm	1215:1221	arg1	properties					1197:1206	the major physicochemical properties	1171:1206	the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes	1171:1327	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	5	15	theme	unhindered	1082:1091	arg1	diffusion					1093:1101	unhindered diffusion	1082:1101	unhindered diffusion	1082:1101	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion.					
31478651	2	16	theme	biofilm	447:453	arg1	organization					466:477	the biofilm structural organization	443:477	the biofilm structural organization	443:477	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	4	17	theme	probe	902:906	arg1	size					908:911	probe size	902:911	probe size	902:911	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	6	18	theme	selective	1240:1248	arg1	barrier					1260:1266	a selective diffusion barrier	1238:1266	a selective diffusion barrier for nanometer-sized objects	1238:1294	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	6	18	theme	selective	1240:1248	arg1	biofilm					1215:1221	the biofilm	1211:1221	the biofilm	1211:1221	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	1	19	theme	bacterial	204:212	arg1	communities					214:224	bacterial communities	204:224	bacterial communities	204:224	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	19	theme	bacterial	204:212	arg1	barriers					240:247	protective barriers	229:247	protective barriers that can be biochemically modified during the bacterial life cycle	229:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	0	20	theme	Polysaccharide-Based	7:26	arg1	Matrix					36:41	Purely Polysaccharide-Based Biofilm Matrix	0:41	Purely Polysaccharide-Based Biofilm Matrix	0:41	Purely Polysaccharide-Based Biofilm Matrix Provides Size-Selective Diffusion Barriers for Nanoparticles and Bacteriophages.					
31478651	3	21	theme	objects	601:607	arg1	dynamics					573:580	the diffusion dynamics	559:580	the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii	559:747	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	22	theme	molecules	981:989	arg1	diffusion					962:970	free diffusion	957:970	free diffusion of small molecules	957:989	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	3	23	theme	polysaccharide	653:666	arg1	matrices					612:619	matrices	612:619	matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii	612:747	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	24	theme	correlation	763:773	arg1	spectroscopy					775:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	3	25	theme	anionic	645:651	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	25	theme	anionic	645:651	arg1	polysaccharide					653:666	the megadalton-sized anionic polysaccharide	624:666	the megadalton-sized anionic polysaccharide	624:666	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	26	theme	small	975:979	arg1	molecules					981:989	small molecules	975:989	small molecules	975:989	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	2	27	theme	components	429:438	arg1	contributions					394:406	the contributions	390:406	the contributions of individual matrix components to the biofilm structural organization	390:477	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	3	28	theme	megadalton-sized	628:643	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	28	theme	megadalton-sized	628:643	arg1	polysaccharide					653:666	the megadalton-sized anionic polysaccharide	624:666	the megadalton-sized anionic polysaccharide	624:666	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	1	29	theme	protective	229:238	arg1	communities					214:224	bacterial communities	204:224	bacterial communities	204:224	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	29	theme	protective	229:238	arg1	barriers					240:247	protective barriers	229:247	protective barriers that can be biochemically modified during the bacterial life cycle	229:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	0	30	theme	Biofilm	28:34	arg1	Matrix					36:41	Purely Polysaccharide-Based Biofilm Matrix	0:41	Purely Polysaccharide-Based Biofilm Matrix	0:41	Purely Polysaccharide-Based Biofilm Matrix Provides Size-Selective Diffusion Barriers for Nanoparticles and Bacteriophages.					
31478651	1	31	theme	complex	137:143	arg1	Biofilms					124:131	Biofilms	124:131	Biofilms	124:131	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	31	theme	complex	137:143	arg1	mixtures					145:152	complex mixtures	137:152	complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle	137:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	32	theme	DNA	167:169	arg1	Biofilms					124:131	Biofilms	124:131	Biofilms	124:131	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	32	theme	DNA	167:169	arg1	mixtures					145:152	complex mixtures	137:152	complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle	137:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	0	33	theme	Size-Selective	52:65	arg1	Barriers					77:84	Size-Selective Diffusion Barriers	52:84	Size-Selective Diffusion Barriers	52:84	Purely Polysaccharide-Based Biofilm Matrix Provides Size-Selective Diffusion Barriers for Nanoparticles and Bacteriophages.					
31478651	3	34	theme	Pantoea	731:737	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	34	theme	Pantoea	731:737	arg1	stewartii					739:747	Pantoea stewartii	731:747	Pantoea stewartii	731:747	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	35	theme	Fluorescence	750:761	arg1	spectroscopy					775:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	3	36	theme	structural	499:508	arg1	properties					510:519	the structural properties	495:519	the structural properties of glycan-based biofilms	495:544	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	5	37	theme	enzymatic	1002:1010	arg1	depolymerization					1012:1027	Stewartan enzymatic depolymerization	992:1027	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins	992:1063	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion.					
31478651	3	38	theme	plant	715:719	arg1	pathogen					721:728	the plant pathogen	711:728	the plant pathogen	711:728	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	39	theme	major	684:688	arg1	component					698:706	the major biofilm component	680:706	the major biofilm component of the plant pathogen	680:728	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	39	theme	major	684:688	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	2	40	theme	structural	455:464	arg1	organization					466:477	the biofilm structural organization	443:477	the biofilm structural organization	443:477	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	3	41	theme	pathogen	721:728	arg1	component					698:706	the major biofilm component	680:706	the major biofilm component of the plant pathogen	680:728	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	41	theme	pathogen	721:728	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	42	theme	free	957:960	arg1	diffusion					962:970	free diffusion	957:970	free diffusion of small molecules	957:989	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	4	43	theme	nanobeads	820:828	arg1	spectroscopy					775:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	4	43	theme	nanobeads	820:828	arg1	tracking					808:815	single-particle tracking	792:815	single-particle tracking	792:815	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	0	44	theme	Diffusion	67:75	arg1	Barriers					77:84	Size-Selective Diffusion Barriers	52:84	Size-Selective Diffusion Barriers	52:84	Purely Polysaccharide-Based Biofilm Matrix Provides Size-Selective Diffusion Barriers for Nanoparticles and Bacteriophages.					
31478651	3	45	theme	diffusion	563:571	arg1	dynamics					573:580	the diffusion dynamics	559:580	the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii	559:747	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	4	46	theme	stewartan	917:925	arg1	concentration					927:939	stewartan concentration	917:939	stewartan concentration	917:939	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	6	47	theme	physicochemical	1181:1195	arg1	properties					1197:1206	the major physicochemical properties	1171:1206	the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes	1171:1327	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	1	48	theme	proteins	157:164	arg1	Biofilms					124:131	Biofilms	124:131	Biofilms	124:131	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	1	48	theme	proteins	157:164	arg1	mixtures					145:152	complex mixtures	137:152	complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle	137:314	Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle.					
31478651	6	49	theme	nanometer-sized	1272:1286	arg1	objects					1288:1294	nanometer-sized objects	1272:1294	nanometer-sized objects	1272:1294	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	4	50	theme	subdiffusive	867:878	arg1	dynamics					880:887	notable subdiffusive dynamics	859:887	notable subdiffusive dynamics dependent on probe size and stewartan concentration	859:939	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	2	51	theme	precise	370:376	arg1	analysis					378:385	a precise analysis	368:385	a precise analysis of the contributions of individual matrix components to the biofilm structural organization	368:477	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	4	52	theme	bacteriophages	834:847	arg1	spectroscopy					775:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy	750:786	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	4	52	theme	bacteriophages	834:847	arg1	tracking					808:815	single-particle tracking	792:815	single-particle tracking	792:815	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	3	53	theme	glycan-based	524:535	arg1	biofilms					537:544	glycan-based biofilms	524:544	glycan-based biofilms	524:544	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	54	theme	biofilm	690:696	arg1	component					698:706	the major biofilm component	680:706	the major biofilm component of the plant pathogen	680:728	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	54	theme	biofilm	690:696	arg1	stewartan					669:677	stewartan	669:677	stewartan	669:677	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	3	55	theme	biofilms	537:544	arg1	properties					510:519	the structural properties	495:519	the structural properties of glycan-based biofilms	495:544	To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii.					
31478651	2	56	theme	matrix	422:427	arg1	components					429:438	individual matrix components	411:438	individual matrix components	411:438	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
31478651	5	57	theme	Stewartan	992:1000	arg1	depolymerization					1012:1027	Stewartan enzymatic depolymerization	992:1027	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins	992:1063	Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion.					
31478651	6	58	theme	major	1175:1179	arg1	properties					1197:1206	the major physicochemical properties	1171:1206	the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes	1171:1327	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	6	59	theme	polymer	1142:1148	arg1	stewartan					1150:1158	the glycan polymer stewartan	1131:1158	the glycan polymer stewartan	1131:1158	We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.					
31478651	4	60	dep	diffusion	962:970	arg1	contrast					945:952	contrast	945:952	contrast	945:952	Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules.					
31478651	2	61	theme	individual	411:420	arg1	components					429:438	individual matrix components	411:438	individual matrix components	411:438	However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization.					
29884769	6	0	dep	SC84	970:973	arg1	ST7					976:978	ST7	976:978	ST7	976:978	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	0	dep	SC84	970:973	arg1	virulence					991:999	very high virulence	981:999	very high virulence	981:999	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	1	theme	1,2-linked	1015:1024	arg1	residue					1035:1041	a 1,2-linked α-d-Glcp residue	1013:1041	a 1,2-linked α-d-Glcp residue as branching substituent	1013:1066	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	2	2	theme	S.	331:332	arg1	LTA					339:341	the S. suis LTA	327:341	the S. suis LTA	327:341	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	6	3	from	89-1591	1167:1173	arg1	present					1149:1155	present	1149:1155	present	1149:1155	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	7	4	theme	other	1324:1328	arg1	strains					1334:1340	the other two strains	1320:1340	the other two strains	1320:1340	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	6	5	theme	intermediate	1182:1193	arg1	ST25					1176:1179	ST25	1176:1179	ST25	1176:1179	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	5	theme	intermediate	1182:1193	arg1	virulence					1195:1203	intermediate virulence	1182:1203	intermediate virulence	1182:1203	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	8	6	theme	dendritic	1627:1635	arg1	cells					1637:1641	dendritic cells	1627:1641	dendritic cells	1627:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	3	7	from	origin	620:625	arg1	structures					510:519	the LTA structures	502:519	the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin	502:625	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	8	8	from	mutants	1391:1397	arg1	preparations					1353:1364	LTA preparations	1349:1364	LTA preparations	1349:1364	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	5	9	theme	ST-specific	824:834	arg1	difference					836:845	a ST-specific difference	822:845	a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs	822:914	We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs.					
29884769	2	10	from	LTA	339:341	arg1	absence					297:303	absence	297:303	absence of d-alanylation from the S. suis LTA	297:341	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	6	11	theme	strain	1160:1165	arg1	89-1591					1167:1173	strain 89-1591	1160:1173	strain 89-1591 (ST25, intermediate virulence)	1160:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	3	12	theme	sequence	583:590	arg1	type					592:595	sequence type	583:595	sequence type (ST)	583:600	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	4	13	attach	present	715:721	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	4	13	attach	present	715:721	arg2	LTA					711:713	the typical type I LTA	692:713	the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity	692:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	7	14	theme	glycerol	1271:1278	arg1	position					1284:1291	the glycerol O-2 position	1267:1291	the glycerol O-2 position	1267:1291	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	1	15	theme	Streptococcus	131:143	arg1	porcine					177:183	an important porcine and human pathogen	164:202	porcine	177:183	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	1	15	theme	Streptococcus	131:143	arg1	serotype					150:157	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	4	16	theme	typical	696:702	arg1	LTA					711:713	the typical type I LTA	692:713	the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity	692:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	6	17	theme	phosphate	1114:1122	arg1	moieties					1124:1131	glycerol phosphate moieties	1105:1131	glycerol phosphate moieties	1105:1131	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	5	18	from	difference	836:845	arg1	incorporation					854:866	the incorporation	850:866	the incorporation of glycosyl residues into these mixed-type LTAs	850:914	We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs.					
29884769	5	19	theme	mixed-type	900:909	arg1	LTAs					911:914	these mixed-type LTAs	894:914	these mixed-type LTAs	894:914	We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs.					
29884769	6	20	dep	P1/7	939:942	arg1	ST1					945:947	ST1	945:947	ST1	945:947	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	20	dep	P1/7	939:942	arg1	virulence					955:963	high virulence	950:963	high virulence	950:963	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	5	21	theme	glycosyl	871:878	arg1	residues					880:887	glycosyl residues	871:887	glycosyl residues	871:887	We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs.					
29884769	6	22	theme	high	950:953	arg1	ST1					945:947	ST1	945:947	ST1	945:947	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	22	theme	high	950:953	arg1	virulence					955:963	high virulence	950:963	high virulence	950:963	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	4	23	theme	other	726:730	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	6	24	attach	1,3-linked	1091:1100	arg1	moieties					1124:1131	glycerol phosphate moieties	1105:1131	glycerol phosphate moieties	1105:1131	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	24	attach	1,3-linked	1091:1100	arg2	1,3-linked					1091:1100	1,3-linked	1091:1100	1,3-linked	1091:1100	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	24	attach	1,3-linked	1091:1100	arg2	α-d-Glcp					1074:1081	an α-d-Glcp	1071:1081	an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence)	1071:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	0	25	theme	suis	106:109	arg1	strains					122:128	three Streptococcus suis serotype 2 strains	86:128	three Streptococcus suis serotype 2 strains	86:128	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	8	26	dep	S.	1521:1522	arg1	suis					1524:1527	suis	1524:1527	suis	1524:1527	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	9	27	theme	LTAs	1702:1705	arg1	complexity					1688:1697	an unexpected complexity	1674:1697	an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence	1674:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	4	28	theme	second	747:752	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	4	29	from	present	715:721	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	8	30	theme	prolipoprotein	1419:1432	arg1	transferase					1449:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	4	31	theme	I	709:709	arg1	LTA					711:713	the typical type I LTA	692:713	the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity	692:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	0	32	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	7	33	located	observed	1308:1315	arg1	strains					1334:1340	the other two strains	1320:1340	the other two strains	1320:1340	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	7	33	located	observed	1308:1315	arg2	position					1284:1291	the glycerol O-2 position	1267:1291	the glycerol O-2 position	1267:1291	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	8	34	theme	WT	1371:1372	arg1	strains					1374:1380	WT strains	1371:1380	WT strains	1371:1380	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	9	35	theme	S.	1724:1725	arg1	strains					1743:1749	three S. suis serotype 2 strains	1718:1749	three S. suis serotype 2 strains differing in genetic background and virulence	1718:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	4	36	theme	present	715:721	arg1	LTA					711:713	the typical type I LTA	692:713	the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity	692:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	9	37	theme	serotype	1732:1739	arg1	strains					1743:1749	three S. suis serotype 2 strains	1718:1749	three S. suis serotype 2 strains differing in genetic background and virulence	1718:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	2	38	theme	cationic	390:397	arg1	peptides					413:420	cationic antimicrobial peptides	390:420	cationic antimicrobial peptides	390:420	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	4	39	theme	molecules	780:788	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	3	40	theme	high-resolution	435:449	arg1	spectroscopy					455:466	high-resolution NMR spectroscopy	435:466	high-resolution NMR spectroscopy	435:466	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	8	41	theme	deficient	1475:1483	arg1	acylation					1497:1505	deficient lipoprotein acylation	1475:1505	deficient lipoprotein acylation	1475:1505	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	4	42	theme	high	793:796	arg1	complexity					798:807	high complexity	793:807	high complexity	793:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	0	43	theme	lipoteichoic	53:64	arg1	acids					66:70	lipoteichoic acids	53:70	lipoteichoic acids	53:70	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	6	44	located	present	1149:1155	arg1	89-1591					1167:1173	strain 89-1591	1160:1173	strain 89-1591 (ST25, intermediate virulence)	1160:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	44	located	present	1149:1155	arg2	that					1137:1140	that	1137:1140	that	1137:1140	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	8	45	from	cells	1637:1641	arg1	production					1611:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production	1554:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	3	46	theme	MS	472:473	arg1	analyses					475:482	MS analyses	472:482	MS analyses	472:482	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	3	47	theme	LTA	506:508	arg1	structures					510:519	the LTA structures	502:519	the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin	502:625	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	8	48	theme	Toll-like	1554:1562	arg1	receptor					1564:1571	Toll-like receptor	1554:1571	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	6	49	dep	89-1591	1167:1173	arg1	ST25					1176:1179	ST25	1176:1179	ST25	1176:1179	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	49	dep	89-1591	1167:1173	arg1	virulence					1195:1203	intermediate virulence	1182:1203	intermediate virulence	1182:1203	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	9	50	from	strains	1743:1749	arg1	present					1707:1713	present	1707:1713	present	1707:1713	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	6	51	theme	α-d-Glcp	1026:1033	arg1	residue					1035:1041	a 1,2-linked α-d-Glcp residue	1013:1041	a 1,2-linked α-d-Glcp residue as branching substituent	1013:1066	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	8	52	theme	2-dependent	1573:1583	arg1	production					1611:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production	1554:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	3	53	theme	S.	532:533	arg1	strains					551:557	serotype 2 strains	540:557	serotype 2 strains differing in virulence	540:580	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	8	54	theme	mediator	1602:1609	arg1	production					1611:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production	1554:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	6	55	theme	glycerol	1105:1112	arg1	moieties					1124:1131	glycerol phosphate moieties	1105:1131	glycerol phosphate moieties	1105:1131	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	56	attach	attach	1006:1011	arg2	strains					931:937	strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence)	931:1000	strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence)	931:1000	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	56	attach	attach	1006:1011	arg2	P1/7					939:942	P1/7	939:942	P1/7	939:942	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	56	attach	attach	1006:1011	arg1	1,3-linked					1091:1100	1,3-linked	1091:1100	1,3-linked	1091:1100	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	56	attach	attach	1006:1011	arg1	α-d-Glcp					1074:1081	an α-d-Glcp	1071:1081	an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence)	1071:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	56	attach	attach	1006:1011	arg2	SC84					970:973	SC84	970:973	SC84	970:973	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	4	57	theme	complexity	798:807	arg1	molecules					780:788	LTA molecules	776:788	LTA molecules of high complexity	776:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	1	58	theme	human	189:193	arg1	pathogen					195:202	an important porcine and human pathogen	164:202	pathogen	195:202	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	3	59	theme	serotype	540:547	arg1	strains					551:557	serotype 2 strains	540:557	serotype 2 strains differing in virulence	540:580	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	3	60	dep	type	592:595	arg1	ST					598:599	ST	598:599	ST	598:599	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	1	61	theme	important	167:175	arg1	porcine					177:183	an important porcine and human pathogen	164:202	porcine	177:183	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	1	61	theme	important	167:175	arg1	serotype					150:157	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	8	62	theme	LTA	1349:1351	arg1	preparations					1353:1364	LTA preparations	1349:1364	LTA preparations	1349:1364	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	8	63	from	strains	1374:1380	arg1	preparations					1353:1364	LTA preparations	1349:1364	LTA preparations	1349:1364	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	5	64	theme	residues	880:887	arg1	incorporation					854:866	the incorporation	850:866	the incorporation of glycosyl residues into these mixed-type LTAs	850:914	We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs.					
29884769	6	65	dep	strains	931:937	arg1	SC84					970:973	SC84	970:973	SC84	970:973	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	65	dep	strains	931:937	arg1	P1/7					939:942	P1/7	939:942	P1/7	939:942	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	65	dep	strains	931:937	arg1	strains					931:937	strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence)	931:1000	strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence)	931:1000	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	7	66	theme	O-2	1280:1282	arg1	position					1284:1291	the glycerol O-2 position	1267:1291	the glycerol O-2 position	1267:1291	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	6	67	from	present	1149:1155	arg1	89-1591					1167:1173	strain 89-1591	1160:1173	strain 89-1591 (ST25, intermediate virulence)	1160:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	68	attach	present	1149:1155	arg1	89-1591					1167:1173	strain 89-1591	1160:1173	strain 89-1591 (ST25, intermediate virulence)	1160:1204	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	68	attach	present	1149:1155	arg2	that					1137:1140	that	1137:1140	that	1137:1140	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	1	69	theme	suis	145:148	arg1	porcine					177:183	an important porcine and human pathogen	164:202	porcine	177:183	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	1	69	theme	suis	145:148	arg1	serotype					150:157	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2	131:159	Streptococcus suis serotype 2 is an important porcine and human pathogen.					
29884769	9	70	attach	present	1707:1713	arg2	LTAs					1702:1705	LTAs	1702:1705	LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence	1702:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	9	70	attach	present	1707:1713	arg1	strains					1743:1749	three S. suis serotype 2 strains	1718:1749	three S. suis serotype 2 strains differing in genetic background and virulence	1718:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	3	71	from	strains	551:557	arg1	structures					510:519	the LTA structures	502:519	the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin	502:625	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	2	72	theme	d-alanylation	308:320	arg1	absence					297:303	absence	297:303	absence of d-alanylation from the S. suis LTA	297:341	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	4	73	from	series	766:771	arg1	present					715:721	present	715:721	present	715:721	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	4	74	dep	second	747:752	arg1	mixed-type					755:764	mixed-type	755:764	mixed-type	755:764	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	0	75	theme	Streptococcus	92:104	arg1	strains					122:128	three Streptococcus suis serotype 2 strains	86:128	three Streptococcus suis serotype 2 strains	86:128	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	3	76	dep	strains	551:557	arg1	suis					535:538	three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin	526:625	suis	535:538	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	9	77	theme	unexpected	1677:1686	arg1	complexity					1688:1697	an unexpected complexity	1674:1697	an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence	1674:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	0	78	theme	serotype	111:118	arg1	strains					122:128	three Streptococcus suis serotype 2 strains	86:128	three Streptococcus suis serotype 2 strains	86:128	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	8	79	theme	inactivated	1407:1417	arg1	transferase					1449:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	2	80	dep	S.	331:332	arg1	suis					334:337	suis	334:337	suis	334:337	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	3	81	from	type	592:595	arg1	structures					510:519	the LTA structures	502:519	the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin	502:625	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	7	82	gly	glycosylate	1244:1254	arg1	LTA					1260:1262	its LTA	1256:1262	its LTA	1256:1262	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	9	83	theme	present	1707:1713	arg1	LTAs					1702:1705	LTAs	1702:1705	LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence	1702:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	8	84	theme	diacylglyceryl	1434:1447	arg1	transferase					1449:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	4	85	theme	streptococci-a	732:745	arg1	series					766:771	other streptococci-a second, mixed-type series	726:771	other streptococci-a second, mixed-type series of LTA molecules of high complexity	726:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	2	86	theme	increased	362:370	arg1	susceptibility					372:385	increased susceptibility	362:385	increased susceptibility to cationic antimicrobial peptides	362:420	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	7	87	theme	latter	1224:1229	arg1	strain					1231:1236	the latter strain	1220:1236	the latter strain	1220:1236	In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains.					
29884769	4	88	theme	type	704:707	arg1	LTA					711:713	the typical type I LTA	692:713	the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity	692:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	0	89	theme	immunostimulatory	24:40	arg1	potency					42:48	immunostimulatory potency	24:48	immunostimulatory potency	24:48	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	2	90	from	suis	237:240	arg1	LTA					224:226	LTA	224:226	LTA	224:226	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	2	90	from	suis	237:240	arg1	acid					218:221	Lipoteichoic acid	205:221	Lipoteichoic acid (LTA) from S. suis	205:240	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	6	91	theme	high	986:989	arg1	ST7					976:978	ST7	976:978	ST7	976:978	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	91	theme	high	986:989	arg1	virulence					991:999	very high virulence	981:999	very high virulence	981:999	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	9	92	theme	suis	1727:1730	arg1	strains					1743:1749	three S. suis serotype 2 strains	1718:1749	three S. suis serotype 2 strains differing in genetic background and virulence	1718:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	3	93	theme	geographical	607:618	arg1	origin					620:625	geographical origin	607:625	geographical origin	607:625	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	2	94	theme	antimicrobial	399:411	arg1	peptides					413:420	cationic antimicrobial peptides	390:420	cationic antimicrobial peptides	390:420	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	9	95	from	present	1707:1713	arg1	strains					1743:1749	three S. suis serotype 2 strains	1718:1749	three S. suis serotype 2 strains differing in genetic background and virulence	1718:1795	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	8	96	theme	lipoprotein	1485:1495	arg1	acylation					1497:1505	deficient lipoprotein acylation	1475:1505	deficient lipoprotein acylation	1475:1505	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	4	97	theme	LTA	776:778	arg1	molecules					780:788	LTA molecules	776:788	LTA molecules of high complexity	776:807	Our analyses revealed that these strains possess-in addition to the typical type I LTA present in other streptococci-a second, mixed-type series of LTA molecules of high complexity.					
29884769	3	98	theme	NMR	451:453	arg1	spectroscopy					455:466	high-resolution NMR spectroscopy	435:466	high-resolution NMR spectroscopy	435:466	Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin.					
29884769	0	99	theme	acids	66:70	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	0	99	theme	acids	66:70	arg1	potency					42:48	immunostimulatory potency	24:48	immunostimulatory potency	24:48	Structural analysis and immunostimulatory potency of lipoteichoic acids isolated from three Streptococcus suis serotype 2 strains.					
29884769	6	100	link	1,2-linked	1015:1024	arg1	residue					1035:1041	a 1,2-linked α-d-Glcp residue	1013:1041	a 1,2-linked α-d-Glcp residue as branching substituent	1013:1066	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	6	101	theme	branching	1046:1054	arg1	substituent					1056:1066	branching substituent	1046:1066	branching substituent	1046:1066	We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-d-Glcp residue as branching substituent to an α-d-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence).					
29884769	9	102	theme	genetic	1764:1770	arg1	background					1772:1781	genetic background	1764:1781	genetic background	1764:1781	In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.					
29884769	2	103	theme	Lipoteichoic	205:216	arg1	LTA					224:226	LTA	224:226	LTA	224:226	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	2	103	theme	Lipoteichoic	205:216	arg1	acid					218:221	Lipoteichoic acid	205:221	Lipoteichoic acid (LTA) from S. suis	205:240	Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides.					
29884769	8	104	theme	receptor	1564:1571	arg1	production					1611:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production	1554:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	8	105	with	mutants	1391:1397	arg1	transferase					1449:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	an inactivated prolipoprotein diacylglyceryl transferase	1404:1459	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	8	106	theme	S.	1521:1522	arg1	LTAs					1529:1532	S. suis LTAs	1521:1532	S. suis LTAs	1521:1532	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
29884769	8	107	theme	pro-inflammatory	1585:1600	arg1	production					1611:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production	1554:1620	Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells	1554:1641	Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2-dependent pro-inflammatory mediator production from dendritic cells.					
31122132	1	0	theme	pathogens	253:261	arg1	activity					218:225	their superior receptor-mediated cytotoxic activity	175:225	their superior receptor-mediated cytotoxic activity	175:225	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	0	theme	pathogens	253:261	arg1	neutralization					235:248	potent neutralization	228:248	potent neutralization of pathogens	228:261	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	0	theme	pathogens	253:261	arg1	ability					268:274	ability	268:274	ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs	268:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	2	1	theme	glycan	609:614	arg1	scavenging					625:634	glycan receptor scavenging	609:634	glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans	609:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	5	2	from	IgA	1318:1320	arg1	mice					1325:1328	mice	1325:1328	mice	1325:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	6	3	theme	systemic	1570:1577	arg1	exposure					1579:1586	low systemic exposure	1566:1586	low systemic exposure	1566:1586	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	2	4	theme	rapid	553:557	arg1	clearance					565:573	rapid serum clearance	553:573	rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans	553:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	4	5	theme	IgG-IgA	1126:1132	arg1	fusion					1137:1142	a polymeric IgG-IgA Fc fusion	1114:1142	a polymeric IgG-IgA Fc fusion	1114:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	3	6	theme	comprehensive	743:755	arg1	characterization					798:813	a comprehensive biochemical, biophysical, and structural characterization	741:813	a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA	741:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	1	7	theme	superior	181:188	arg1	activity					218:225	their superior receptor-mediated cytotoxic activity	175:225	their superior receptor-mediated cytotoxic activity	175:225	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	4	8	theme	polymeric	960:968	arg1	IgA					970:972	polymeric IgA	960:972	polymeric IgA	960:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	9	theme	fusion	1137:1142	arg1	generation					1100:1109	the generation	1096:1109	the generation of a polymeric IgG-IgA Fc fusion	1096:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	3	10	theme	structural	787:796	arg1	characterization					798:813	a comprehensive biochemical, biophysical, and structural characterization	741:813	a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA	741:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	4	11	from	removal	975:981	arg1	FcRn					1078:1081	FcRn	1078:1081	FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1078:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	1	12	theme	traditional	392:402	arg1	drugs					414:418	traditional IgG-based drugs	392:418	traditional IgG-based drugs	392:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	13	theme	cytotoxic	208:216	arg1	activity					218:225	their superior receptor-mediated cytotoxic activity	175:225	their superior receptor-mediated cytotoxic activity	175:225	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	0	14	from	extension	52:60	arg1	mice					92:95	mice	92:95	mice	92:95	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	5	15	from	mice	1325:1328	arg1	clearance					1295:1303	systemic clearance	1286:1303	systemic clearance of polymeric IgA in mice	1286:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	4	16	theme	rapid	935:939	arg1	clearance					947:955	the rapid serum clearance	931:955	the rapid serum clearance of polymeric IgA	931:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	2	17	theme	N-linked	706:713	arg1	glycans					715:721	incompletely sialylated N-linked glycans	682:721	incompletely sialylated N-linked glycans	682:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	5	18	theme	polymeric	1308:1316	arg1	IgA					1318:1320	polymeric IgA	1308:1320	polymeric IgA in mice	1308:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	3	19	theme	produced	832:839	arg1	IgA					880:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	1	20	theme	broad	118:122	arg1	platform					157:164	a novel therapeutic platform	137:164	a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs	137:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	20	theme	broad	118:122	arg1	potential					124:132	broad potential	118:132	broad potential	118:132	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	3	21	theme	human	874:878	arg1	IgA					880:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	4	22	theme	N-linked	990:997	arg1	sites					1013:1017	all N-linked glycosylation sites	986:1017	all N-linked glycosylation sites creating an aglycosylated polymeric IgA	986:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	1	23	theme	immunoglobulin	329:342	arg1	receptor					344:351	polymeric immunoglobulin receptor	319:351	polymeric immunoglobulin receptor	319:351	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	4	24	theme	sites	1013:1017	arg1	removal					975:981	removal	975:981	removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA	975:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	24	theme	sites	1013:1017	arg1	clearance					947:955	the rapid serum clearance	931:955	the rapid serum clearance of polymeric IgA	931:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	24	theme	sites	1013:1017	arg1	engineering					1063:1073	engineering	1063:1073	engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1063:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	0	25	from	characterization	12:27	arg1	mice					92:95	mice	92:95	mice	92:95	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	0	26	theme	polymeric	65:73	arg1	molecules					79:87	polymeric IgA molecules	65:87	polymeric IgA molecules	65:87	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	1	27	theme	novel	139:143	arg1	platform					157:164	a novel therapeutic platform	137:164	a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs	137:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	27	theme	novel	139:143	arg1	potential					124:132	broad potential	118:132	broad potential	118:132	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	0	28	from	Production	0:9	arg1	mice					92:95	mice	92:95	mice	92:95	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	2	29	theme	produced	653:660	arg1	monomer					666:672	recombinantly produced IgA monomer	639:672	recombinantly produced IgA monomer	639:672	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	0	30	theme	molecules	79:87	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	0	30	theme	molecules	79:87	arg1	Production					0:9	Production	0:9	Production	0:9	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	0	30	theme	molecules	79:87	arg1	extension					52:60	in vivo half-life extension	34:60	in vivo half-life extension	34:60	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	6	31	theme	IgA	1460:1462	arg1	platform					1464:1471	The developed IgA platform	1446:1471	The developed IgA platform	1446:1471	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	2	32	theme	IgA	448:450	arg1	transition					434:443	the transition	430:443	the transition of IgA into clinical development	430:476	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	2	33	theme	complex	501:507	arg1	expression					509:518	complex expression	501:518	complex expression	501:518	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	2	34	theme	monomer	666:672	arg1	scavenging					625:634	glycan receptor scavenging	609:634	glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans	609:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	4	35	link	N-linked	990:997	arg1	sites					1013:1017	all N-linked glycosylation sites	986:1017	all N-linked glycosylation sites creating an aglycosylated polymeric IgA	986:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	6	36	theme	developed	1450:1458	arg1	platform					1464:1471	The developed IgA platform	1446:1471	The developed IgA platform	1446:1471	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	1	37	theme	mucosal	298:304	arg1	barriers					306:313	mucosal barriers	298:313	mucosal barriers	298:313	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	4	38	theme	polymeric	1045:1053	arg1	IgA					1055:1057	an aglycosylated polymeric IgA	1028:1057	an aglycosylated polymeric IgA	1028:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	1	39	dep	-mediated	359:367	arg1	receptor					344:351	polymeric immunoglobulin receptor	319:351	polymeric immunoglobulin receptor	319:351	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	5	40	theme	major	1256:1260	arg1	role					1262:1265	a major role	1254:1265	a major role for monomeric IgA	1254:1283	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	4	41	theme	polymeric	1116:1124	arg1	fusion					1137:1142	a polymeric IgG-IgA Fc fusion	1114:1142	a polymeric IgG-IgA Fc fusion	1114:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	5	42	theme	receptor	1390:1397	arg1	clearance					1399:1407	glycan receptor clearance	1383:1407	glycan receptor clearance	1383:1407	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	6	43	theme	pIgR	1523:1526	arg1	tissues					1539:1545	target pIgR expressing tissues	1516:1545	target pIgR expressing tissues	1516:1545	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	1	44	theme	potent	228:233	arg1	neutralization					235:248	potent neutralization	228:248	potent neutralization of pathogens	228:261	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	2	45	theme	clinical	457:464	arg1	development					466:476	clinical development	457:476	clinical development	457:476	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	4	46	attach	removal	975:981	arg3	FcRn					1078:1081	FcRn	1078:1081	FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1078:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	46	attach	removal	975:981	arg2	sites					1013:1017	all N-linked glycosylation sites	986:1017	all N-linked glycosylation sites creating an aglycosylated polymeric IgA	986:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	46	attach	removal	975:981	arg2	IgA					970:972	polymeric IgA	960:972	polymeric IgA	960:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	1	47	contain	have	113:116	arg1	antibodies					102:111	IgA antibodies	98:111	IgA antibodies	98:111	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	47	contain	have	113:116	arg2	platform					157:164	a novel therapeutic platform	137:164	a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs	137:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	47	contain	have	113:116	arg2	potential					124:132	broad potential	118:132	broad potential	118:132	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	5	48	theme	glycan-mediated	1222:1236	arg1	clearance					1238:1246	glycan-mediated clearance	1222:1246	glycan-mediated clearance	1222:1246	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	5	49	from	clearance	1295:1303	arg1	mice					1325:1328	mice	1325:1328	mice	1325:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	1	50	theme	therapeutic	145:155	arg1	platform					157:164	a novel therapeutic platform	137:164	a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs	137:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	50	theme	therapeutic	145:155	arg1	potential					124:132	broad potential	118:132	broad potential	118:132	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	2	51	theme	receptor	616:623	arg1	scavenging					625:634	glycan receptor scavenging	609:634	glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans	609:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	4	52	theme	Fc	1134:1135	arg1	fusion					1137:1142	a polymeric IgG-IgA Fc fusion	1114:1142	a polymeric IgG-IgA Fc fusion	1114:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	3	53	theme	biophysical	770:780	arg1	characterization					798:813	a comprehensive biochemical, biophysical, and structural characterization	741:813	a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA	741:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	6	54	theme	low	1566:1568	arg1	exposure					1579:1586	low systemic exposure	1566:1586	low systemic exposure	1566:1586	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	4	55	theme	serum	941:945	arg1	clearance					947:955	the rapid serum clearance	931:955	the rapid serum clearance of polymeric IgA	931:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	3	56	theme	biochemical	757:767	arg1	characterization					798:813	a comprehensive biochemical, biophysical, and structural characterization	741:813	a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA	741:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	1	57	theme	receptor-mediated	190:206	arg1	activity					218:225	their superior receptor-mediated cytotoxic activity	175:225	their superior receptor-mediated cytotoxic activity	175:225	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	58	theme	IgG-based	404:412	arg1	drugs					414:418	traditional IgG-based drugs	392:418	traditional IgG-based drugs	392:418	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	1	59	theme	IgA	98:100	arg1	antibodies					102:111	IgA antibodies	98:111	IgA antibodies	98:111	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	2	60	theme	serum	559:563	arg1	clearance					565:573	rapid serum clearance	553:573	rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans	553:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	5	61	theme	IgA	1318:1320	arg1	clearance					1295:1303	systemic clearance	1286:1303	systemic clearance of polymeric IgA in mice	1286:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	3	62	theme	monomeric	841:849	arg1	IgA					880:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	3	63	theme	IgA	880:882	arg1	characterization					798:813	a comprehensive biochemical, biophysical, and structural characterization	741:813	a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA	741:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	2	64	link	N-linked	706:713	arg1	glycans					715:721	incompletely sialylated N-linked glycans	682:721	incompletely sialylated N-linked glycans	682:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	0	65	theme	IgA	75:77	arg1	molecules					79:87	polymeric IgA molecules	65:87	polymeric IgA molecules	65:87	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	5	66	theme	previous	1151:1158	arg1	reports					1160:1166	previous reports	1151:1166	previous reports	1151:1166	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	4	67	theme	glycosylation	999:1011	arg1	sites					1013:1017	all N-linked glycosylation sites	986:1017	all N-linked glycosylation sites creating an aglycosylated polymeric IgA	986:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	2	68	theme	IgA	662:664	arg1	monomer					666:672	recombinantly produced IgA monomer	639:672	recombinantly produced IgA monomer	639:672	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	5	69	theme	systemic	1286:1293	arg1	clearance					1295:1303	systemic clearance	1286:1303	systemic clearance of polymeric IgA in mice	1286:1328	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	3	70	theme	polymeric	864:872	arg1	IgA					880:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	4	71	theme	IgA	970:972	arg1	removal					975:981	removal	975:981	removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA	975:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	71	theme	IgA	970:972	arg1	clearance					947:955	the rapid serum clearance	931:955	the rapid serum clearance of polymeric IgA	931:972	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	4	71	theme	IgA	970:972	arg1	engineering					1063:1073	engineering	1063:1073	engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1063:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	3	72	theme	dimeric	852:858	arg1	IgA					880:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	recombinantly produced monomeric, dimeric and polymeric human IgA	818:882	Here, we present a comprehensive biochemical, biophysical, and structural characterization of recombinantly produced monomeric, dimeric and polymeric human IgA.					
31122132	1	73	theme	-mediated	359:367	arg1	transport					369:377	polymeric immunoglobulin receptor (pIgR)-mediated transport	319:377	polymeric immunoglobulin receptor (pIgR)-mediated transport	319:377	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	4	74	gly	glycosylation	999:1011	arg2	sites					1013:1017	all N-linked glycosylation sites	986:1017	all N-linked glycosylation sites creating an aglycosylated polymeric IgA	986:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	2	75	theme	sialylated	695:704	arg1	glycans					715:721	incompletely sialylated N-linked glycans	682:721	incompletely sialylated N-linked glycans	682:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	5	76	theme	other	1372:1376	arg1	mechanisms					1361:1370	mechanisms	1361:1370	mechanisms	1361:1370	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	5	76	theme	other	1372:1376	arg1	transcytosis					1432:1443	pIgR-mediated transcytosis	1418:1443	pIgR-mediated transcytosis	1418:1443	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	4	77	from	engineering	1063:1073	arg1	FcRn					1078:1081	FcRn	1078:1081	FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1078:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	0	78	theme	in	34:35	arg1	extension					52:60	in vivo half-life extension	34:60	in vivo half-life extension	34:60	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	0	79	theme	half-life	42:50	arg1	extension					52:60	in vivo half-life extension	34:60	in vivo half-life extension	34:60	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	0	80	dep	in	34:35	arg1	vivo					37:40	vivo	37:40	vivo	37:40	Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice.					
31122132	4	81	theme	aglycosylated	1031:1043	arg1	IgA					1055:1057	an aglycosylated polymeric IgA	1028:1057	an aglycosylated polymeric IgA	1028:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	5	82	theme	pIgR-mediated	1418:1430	arg1	transcytosis					1432:1443	pIgR-mediated transcytosis	1418:1443	pIgR-mediated transcytosis	1418:1443	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	4	83	from	clearance	947:955	arg1	FcRn					1078:1081	FcRn	1078:1081	FcRn binding with the generation of a polymeric IgG-IgA Fc fusion	1078:1142	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	1	84	theme	polymeric	319:327	arg1	receptor					344:351	polymeric immunoglobulin receptor	319:351	polymeric immunoglobulin receptor	319:351	IgA antibodies have broad potential as a novel therapeutic platform based on their superior receptor-mediated cytotoxic activity, potent neutralization of pathogens, and ability to transcytose across mucosal barriers via polymeric immunoglobulin receptor (pIgR)-mediated transport, compared to traditional IgG-based drugs.					
31122132	5	85	theme	glycan	1383:1388	arg1	clearance					1399:1407	glycan receptor clearance	1383:1407	glycan receptor clearance	1383:1407	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31122132	6	86	theme	expressing	1528:1537	arg1	tissues					1539:1545	target pIgR expressing tissues	1516:1545	target pIgR expressing tissues	1516:1545	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	2	87	gly	sialylated	695:704	arg1	glycans					715:721	incompletely sialylated N-linked glycans	682:721	incompletely sialylated N-linked glycans	682:721	However, the transition of IgA into clinical development has been challenged by complex expression and characterization, as well as rapid serum clearance that is thought to be mediated by glycan receptor scavenging of recombinantly produced IgA monomer bearing incompletely sialylated N-linked glycans.					
31122132	4	88	gly	aglycosylated	1031:1043	arg1	IgA					1055:1057	an aglycosylated polymeric IgA	1028:1057	an aglycosylated polymeric IgA	1028:1057	We further explore two strategies to overcome the rapid serum clearance of polymeric IgA: removal of all N-linked glycosylation sites creating an aglycosylated polymeric IgA and engineering in FcRn binding with the generation of a polymeric IgG-IgA Fc fusion.					
31122132	6	89	theme	target	1516:1521	arg1	tissues					1539:1545	target pIgR expressing tissues	1516:1545	target pIgR expressing tissues	1516:1545	The developed IgA platform now provides the potential to specifically target pIgR expressing tissues, while maintaining low systemic exposure.					
31122132	5	90	theme	monomeric	1271:1279	arg1	IgA					1281:1283	monomeric IgA	1271:1283	monomeric IgA	1271:1283	While previous reports and the results presented in this study indicate that glycan-mediated clearance plays a major role for monomeric IgA, systemic clearance of polymeric IgA in mice is predominantly controlled by mechanisms other than glycan receptor clearance, such as pIgR-mediated transcytosis.					
31754374	10	0	theme	additional	1554:1563	arg1	glycomutants					1565:1576	Six additional glycomutants	1550:1576	Six additional glycomutants	1550:1576	Six additional glycomutants were designed based on BxlBN1;5;7, to better understand its increased activity.					
31754374	8	1	theme	enzyme	1219:1224	arg1	secretion					1226:1234	no enzyme secretion	1216:1234	no enzyme secretion	1216:1234	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	14	2	gly	N-glycosylation	2400:2414	arg1	BxlB					2431:2434	A. nidulans BxlB production	2419:2445	A. nidulans BxlB production	2419:2445	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	4	3	theme	proteins	644:651	arg1	yields					627:632	high secretion yields	612:632	high secretion yields of fungal proteins for industrial applications	612:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	13	4	from	elements	2110:2117	arg1	vicinity					2126:2133	the vicinity	2122:2133	the vicinity of the catalytic pocket	2122:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	7	5	theme	increased	1172:1180	arg1	activity					1182:1189	increased activity	1172:1189	increased activity	1172:1189	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	2	6	theme	carbohydrate-active	311:329	arg1	CAZymes					340:346	CAZymes	340:346	CAZymes	340:346	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	6	theme	carbohydrate-active	311:329	arg1	enzymes					331:337	carbohydrate-active enzymes	311:337	carbohydrate-active enzymes (CAZymes)	311:347	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	9	7	theme	secondary	1451:1459	arg1	structures					1461:1470	similar secondary structures	1443:1470	similar secondary structures	1443:1470	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	14	8	from	influence	2387:2395	arg1	function					2451:2458	function	2451:2458	function	2451:2458	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	14	8	from	influence	2387:2395	arg1	production					2436:2445	A. nidulans BxlB production	2419:2445	A. nidulans BxlB production	2419:2445	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	11	9	theme	improved	1775:1782	arg1	efficiency					1794:1803	improved catalytic efficiency	1775:1803	improved catalytic efficiency	1775:1803	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	15	10	theme	biotechnological	2619:2634	arg1	applications					2636:2647	biotechnological applications	2619:2647	biotechnological applications for N-glycosylation modification of other CAZymes	2619:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	13	11	theme	catalytic	2291:2299	arg1	efficiency					2301:2310	catalytic efficiency	2291:2310	catalytic efficiency	2291:2310	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	8	12	theme	N-glycosylation	1263:1277	arg1	context					1279:1285	the N-glycosylation context	1259:1285	the N-glycosylation context	1259:1285	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	8	13	theme	N-glycosylation	1331:1345	arg1	BxlBCC					1354:1359	BxlBCC	1354:1359	BxlBCC	1354:1359	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	8	13	theme	N-glycosylation	1331:1345	arg1	sites					1347:1351	four new N-glycosylation sites	1322:1351	four new N-glycosylation sites (BxlBCC)	1322:1360	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	10	14	dep	BxlBN1	1601:1606	arg1	7					1610:1610	7	1610:1610	7	1610:1610	Six additional glycomutants were designed based on BxlBN1;5;7, to better understand its increased activity.					
31754374	10	14	dep	BxlBN1	1601:1606	arg1	5					1608:1608	5	1608:1608	5	1608:1608	Six additional glycomutants were designed based on BxlBN1;5;7, to better understand its increased activity.					
31754374	13	15	theme	structural	2099:2108	arg1	elements					2110:2117	structural elements	2099:2117	structural elements in the vicinity of the catalytic pocket	2099:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	9	16	theme	melting	1502:1508	arg1	temperatures					1510:1521	lower melting temperatures	1496:1521	lower melting temperatures	1496:1521	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	4	17	theme	greater	487:493	arg1	understanding					495:507	A greater understanding	485:507	A greater understanding of the N-glycosylation process	485:538	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	7	18	gly	glycosylated	1129:1140	arg1	mutant					1142:1147	a partially glycosylated mutant	1117:1147	a partially glycosylated mutant (BxlBN1;5;7)	1117:1160	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	14	19	theme	protein	2478:2484	arg1	glycoengineering					2486:2501	protein glycoengineering	2478:2501	protein glycoengineering	2478:2501	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	14	19	theme	protein	2478:2484	arg1	tool					2518:2521	a promising tool	2506:2521	a promising tool for enhancing thermal stability, secretion, and enzymatic activity	2506:2588	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	7	20	theme	glycosylated	1129:1140	arg1	mutant					1142:1147	a partially glycosylated mutant	1117:1147	a partially glycosylated mutant (BxlBN1;5;7)	1117:1160	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	11	21	gly	N-glycosylation	1717:1731	arg2	sites					1733:1737	only two N-glycosylation sites	1708:1737	only two N-glycosylation sites	1708:1737	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	11	21	gly	N-glycosylation	1717:1731	arg2	two					1713:1715	two	1713:1715	two	1713:1715	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	2	22	theme	genetic	278:284	arg1	model					286:290	an established genetic model	263:290	an established genetic model	263:290	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	22	theme	genetic	278:284	arg1	nidulans					251:258	Aspergillus nidulans	239:258	Aspergillus nidulans	239:258	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	3	23	theme	Most	350:353	arg1	enzymes					362:368	Most fungal enzymes	350:368	Most fungal enzymes	350:368	Most fungal enzymes are N-glycosylated, which influences their secretion, stability, activity, signalization, and protease protection.					
31754374	8	24	theme	high	1375:1378	arg1	levels					1391:1396	the high transcript levels	1371:1396	the high transcript levels	1371:1396	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	5	25	theme	N-glycosylation	839:853	arg1	engineering					855:865	N-glycosylation engineering	839:865	N-glycosylation engineering	839:865	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	14	26	theme	enzymatic	2571:2579	arg1	activity					2581:2588	enzymatic activity	2571:2588	enzymatic activity	2571:2588	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	13	27	theme	dynamics	2012:2019	arg1	simulations					2021:2031	Molecular dynamics simulations	2002:2031	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5	2002:2060	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	7	28	theme	enzyme	1045:1050	arg1	secretion					1052:1060	enzyme secretion	1045:1060	enzyme secretion	1045:1060	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	14	29	dep	CONCLUSIONS	2347:2357	arg1	demonstrates					2370:2381	demonstrates	2370:2381	demonstrates	2370:2381	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	3	30	theme	protease	464:471	arg1	protection					473:482	protease protection	464:482	protease protection	464:482	Most fungal enzymes are N-glycosylated, which influences their secretion, stability, activity, signalization, and protease protection.					
31754374	5	31	theme	N-glycosylation	800:814	arg1	sites-was					816:824	several N-glycosylation sites-was	792:824	several N-glycosylation sites-was selected for N-glycosylation engineering	792:865	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	4	32	theme	N-glycosylation	516:530	arg1	process					532:538	the N-glycosylation process	512:538	the N-glycosylation process	512:538	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	9	33	contain	had	1492:1494	arg2	temperatures					1510:1521	lower melting temperatures	1496:1521	lower melting temperatures	1496:1521	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	9	33	contain	had	1492:1494	arg1	mutants					1484:1490	the mutants	1480:1490	the mutants	1480:1490	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	12	34	theme	N-glycosylation	1879:1893	arg1	site					1895:1898	N-glycosylation site	1879:1898	The N-glycosylation site N5	1875:1901	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	12	34	theme	N-glycosylation	1879:1893	arg1	important					1906:1914	important	1906:1914	important	1906:1914	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	13	35	from	vicinity	2126:2133	arg1	pattern					2088:2094	the mobility pattern	2075:2094	the mobility pattern of structural elements in the vicinity of the catalytic pocket	2075:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	13	36	gly	N5 N-glycosylation	2179:2196	arg2	sites					2198:2202	N5 N-glycosylation sites	2179:2202	N5 N-glycosylation sites	2179:2202	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	1	37	theme	glycoside	120:128	arg1	GHs					142:144	GHs	142:144	GHs	142:144	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	1	37	theme	glycoside	120:128	arg1	β-Xylosidases					102:114	BACKGROUND β-Xylosidases	91:114	BACKGROUND β-Xylosidases	91:114	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	1	37	theme	glycoside	120:128	arg1	hydrolases					130:139	glycoside hydrolases	120:139	glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose	120:236	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	13	38	theme	binding	2225:2231	arg1	properties					2233:2242	substrate binding properties	2215:2242	substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5	2215:2344	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	4	39	theme	high	612:615	arg1	yields					627:632	high secretion yields	612:632	high secretion yields of fungal proteins for industrial applications	612:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	3	40	gly	N-glycosylated	374:387	arg1	enzymes					362:368	Most fungal enzymes	350:368	Most fungal enzymes	350:368	Most fungal enzymes are N-glycosylated, which influences their secretion, stability, activity, signalization, and protease protection.					
31754374	6	41	theme	BxlB	948:951	arg1	secretion					953:961	BxlB secretion	948:961	BxlB secretion	948:961	Several glycomutants were designed to investigate the influence of N-glycans on BxlB secretion and function.					
31754374	0	42	gly	N-glycosylation	12:26	arg2	sites					28:32	N-glycosylation sites	12:32	N-glycosylation sites	12:32	Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency.					
31754374	5	43	dep	RESULTS	682:688	arg1	secreted					719:726	secreted	719:726	secreted	719:726	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	13	44	dep	BxlBnon-glyc	2036:2047	arg1	5					2060:2060	5	2060:2060	5	2060:2060	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	8	45	gly	N-glycosylation	1331:1345	arg2	sites					1347:1351	four new N-glycosylation sites	1322:1351	four new N-glycosylation sites (BxlBCC)	1322:1360	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	8	45	gly	N-glycosylation	1331:1345	arg2	four					1322:1325	four	1322:1325	four	1322:1325	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	8	45	gly	N-glycosylation	1331:1345	arg2	BxlBCC					1354:1359	BxlBCC	1354:1359	BxlBCC	1354:1359	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	6	46	theme	N-glycans	935:943	arg1	influence					922:930	the influence	918:930	the influence of N-glycans on BxlB secretion and function	918:974	Several glycomutants were designed to investigate the influence of N-glycans on BxlB secretion and function.					
31754374	4	47	theme	current	579:585	arg1	bottlenecks					587:597	the current bottlenecks	575:597	the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications	575:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	13	48	theme	Molecular	2002:2010	arg1	simulations					2021:2031	Molecular dynamics simulations	2002:2031	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5	2002:2060	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	11	49	theme	other	1818:1822	arg1	mutants					1829:1835	the other four mutants	1814:1835	the other four mutants' catalytic efficiencies	1814:1859	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	11	50	dep	glycomutants	1678:1689	arg1	BxlBN1					1745:1750	BxlBN1	1745:1750	BxlBN1	1745:1750	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	11	50	dep	glycomutants	1678:1689	arg1	each					1739:1742	each	1739:1742	each	1739:1742	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	7	51	dep	mutant	1142:1147	arg1	BxlBN1					1150:1155	BxlBN1	1150:1155	BxlBN1;5;7	1150:1159	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	11	52	theme	N-glycosylation	1717:1731	arg1	sites					1733:1737	only two N-glycosylation sites	1708:1737	only two N-glycosylation sites	1708:1737	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	13	53	theme	pocket	2152:2157	arg1	vicinity					2126:2133	the vicinity	2122:2133	the vicinity of the catalytic pocket	2122:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	7	54	dep	BxlBN1	1150:1155	arg1	7					1159:1159	7	1159:1159	7	1159:1159	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	7	54	dep	BxlBN1	1150:1155	arg1	5					1157:1157	5	1157:1157	5	1157:1157	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	11	55	theme	catalytic	1838:1846	arg1	efficiencies					1848:1859	' catalytic efficiencies	1836:1859	the other four mutants' catalytic efficiencies	1814:1859	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	7	56	theme	non-glycosylated	981:996	arg1	BxlBnon-glyc					1006:1017	BxlBnon-glyc	1006:1017	BxlBnon-glyc	1006:1017	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	7	56	theme	non-glycosylated	981:996	arg1	mutant					998:1003	The non-glycosylated mutant	977:1003	The non-glycosylated mutant (BxlBnon-glyc)	977:1018	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	13	57	from	differences	2276:2286	arg1	efficiency					2301:2310	catalytic efficiency	2291:2310	catalytic efficiency	2291:2310	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	2	58	theme	enzymes	331:337	arg1	model					286:290	an established genetic model	263:290	an established genetic model	263:290	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	58	theme	enzymes	331:337	arg1	source					301:306	good source	296:306	good source of carbohydrate-active enzymes (CAZymes)	296:347	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	58	theme	enzymes	331:337	arg1	nidulans					251:258	Aspergillus nidulans	239:258	Aspergillus nidulans	239:258	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	9	59	theme	similar	1443:1449	arg1	structures					1461:1470	similar secondary structures	1443:1470	similar secondary structures	1443:1470	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	4	60	theme	fungal	637:642	arg1	proteins					644:651	fungal proteins	637:651	fungal proteins for industrial applications	637:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	13	61	from	pattern	2088:2094	arg1	vicinity					2126:2133	the vicinity	2122:2133	the vicinity of the catalytic pocket	2122:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	12	62	dep	site	1895:1898	arg1	N5					1900:1901	N5	1900:1901	The N-glycosylation site N5	1875:1901	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	6	63	theme	Several	868:874	arg1	glycomutants					876:887	Several glycomutants	868:887	Several glycomutants	868:887	Several glycomutants were designed to investigate the influence of N-glycans on BxlB secretion and function.					
31754374	15	64	theme	N-glycosylation	2653:2667	arg1	modification					2669:2680	N-glycosylation modification	2653:2680	N-glycosylation modification of other CAZymes	2653:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	1	65	theme	shorter	202:208	arg1	oligosaccharides					210:225	shorter oligosaccharides	202:225	shorter oligosaccharides	202:225	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	11	66	theme	catalytic	1784:1792	arg1	efficiency					1794:1803	improved catalytic efficiency	1775:1803	improved catalytic efficiency	1775:1803	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	7	67	gly	non-glycosylated	981:996	arg1	BxlBnon-glyc					1006:1017	BxlBnon-glyc	1006:1017	BxlBnon-glyc	1006:1017	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	7	67	gly	non-glycosylated	981:996	arg1	mutant					998:1003	The non-glycosylated mutant	977:1003	The non-glycosylated mutant (BxlBnon-glyc)	977:1018	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	6	68	from	influence	922:930	arg1	function					967:974	function	967:974	function	967:974	Several glycomutants were designed to investigate the influence of N-glycans on BxlB secretion and function.					
31754374	6	68	from	influence	922:930	arg1	secretion					953:961	BxlB secretion	948:961	BxlB secretion	948:961	Several glycomutants were designed to investigate the influence of N-glycans on BxlB secretion and function.					
31754374	11	69	dep	BxlBN1	1745:1750	arg1	BxlBN5					1758:1763	BxlBN5	1758:1763	BxlBN5	1758:1763	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	11	69	dep	BxlBN1	1745:1750	arg1	5					1752:1752	5	1752:1752	5	1752:1752	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	11	69	dep	BxlBN1	1745:1750	arg1	7					1765:1765	7	1765:1765	7	1765:1765	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	2	70	theme	established	266:276	arg1	model					286:290	an established genetic model	263:290	an established genetic model	263:290	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	70	theme	established	266:276	arg1	nidulans					251:258	Aspergillus nidulans	239:258	Aspergillus nidulans	239:258	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	9	71	theme	lower	1496:1500	arg1	temperatures					1510:1521	lower melting temperatures	1496:1521	lower melting temperatures	1496:1521	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	12	72	theme	improved	1920:1927	arg1	efficiency					1944:1953	improved BxlB catalytic efficiency	1920:1953	improved BxlB catalytic efficiency	1920:1953	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	9	73	dep	BxlBN1	1425:1430	arg1	5					1432:1432	5	1432:1432	5	1432:1432	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	9	73	dep	BxlBN1	1425:1430	arg1	7					1434:1434	7	1434:1434	7	1434:1434	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	8	74	theme	new	1327:1329	arg1	BxlBCC					1354:1359	BxlBCC	1354:1359	BxlBCC	1354:1359	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	8	74	theme	new	1327:1329	arg1	sites					1347:1351	four new N-glycosylation sites	1322:1351	four new N-glycosylation sites (BxlBCC)	1322:1360	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	5	75	theme	GH3	728:730	arg1	β-xylosidase					732:743	GH3 β-xylosidase	728:743	GH3 β-xylosidase	728:743	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	13	76	theme	elements	2110:2117	arg1	pattern					2088:2094	the mobility pattern	2075:2094	the mobility pattern of structural elements in the vicinity of the catalytic pocket	2075:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	12	77	theme	catalytic	1934:1942	arg1	efficiency					1944:1953	improved BxlB catalytic efficiency	1920:1953	improved BxlB catalytic efficiency	1920:1953	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	14	78	dep	A.	2419:2420	arg1	nidulans					2422:2429	nidulans	2422:2429	nidulans	2422:2429	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	8	79	theme	sites	1347:1351	arg1	introduction					1306:1317	the introduction	1302:1317	the introduction of four new N-glycosylation sites (BxlBCC)	1302:1360	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	10	80	dep	designed	1583:1590	arg1	based					1592:1596	based	1592:1596	based	1592:1596	Six additional glycomutants were designed based on BxlBN1;5;7, to better understand its increased activity.					
31754374	2	81	theme	good	296:299	arg1	source					301:306	good source	296:306	good source of carbohydrate-active enzymes (CAZymes)	296:347	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	13	82	theme	observed	2267:2274	arg1	differences					2276:2286	the observed differences	2263:2286	the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5	2263:2344	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	0	83	theme	enzymatic	69:77	arg1	efficiency					79:88	the enzymatic efficiency	65:88	the enzymatic efficiency	65:88	Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency.					
31754374	13	84	theme	mobility	2079:2086	arg1	pattern					2088:2094	the mobility pattern	2075:2094	the mobility pattern of structural elements in the vicinity of the catalytic pocket	2075:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	9	85	theme	wild	1539:1542	arg1	type					1544:1547	the wild type	1535:1547	the wild type	1535:1547	BxlBwt, BxlBnon-glyc, and BxlBN1;5;7 formed similar secondary structures, though the mutants had lower melting temperatures compared to the wild type.					
31754374	7	86	dep	showed	1020:1025	arg1	compared					1075:1082	compared	1075:1082	compared	1075:1082	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	13	87	theme	BxlBN1	2053:2058	arg1	simulations					2021:2031	Molecular dynamics simulations	2002:2031	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5	2002:2060	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	3	88	theme	fungal	355:360	arg1	enzymes					362:368	Most fungal enzymes	350:368	Most fungal enzymes	350:368	Most fungal enzymes are N-glycosylated, which influences their secretion, stability, activity, signalization, and protease protection.					
31754374	12	89	gly	N-glycosylation	1879:1893	arg2	important					1906:1914	important	1906:1914	important	1906:1914	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	12	89	gly	N-glycosylation	1879:1893	arg2	site					1895:1898	N-glycosylation site	1879:1898	The N-glycosylation site N5	1875:1901	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	7	90	theme	activity	1066:1073	arg1	levels					1035:1040	similar levels	1027:1040	similar levels of enzyme secretion and activity	1027:1073	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	15	91	mod	modification	2669:2680	arg1	CAZymes					2691:2697	other CAZymes	2685:2697	other CAZymes	2685:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	15	91	mod	modification	2669:2680	arg3	N-glycosylation					2653:2667	N-glycosylation modification	2653:2680	N-glycosylation modification of other CAZymes	2653:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	8	92	theme	transcript	1380:1389	arg1	levels					1391:1396	the high transcript levels	1371:1396	the high transcript levels	1371:1396	Additionally, there was no enzyme secretion in the mutant in which the N-glycosylation context was changed by the introduction of four new N-glycosylation sites (BxlBCC), despite the high transcript levels.					
31754374	5	93	theme	several	792:798	arg1	sites-was					816:824	several N-glycosylation sites-was	792:824	several N-glycosylation sites-was selected for N-glycosylation engineering	792:865	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	7	94	theme	secretion	1052:1060	arg1	levels					1035:1040	similar levels	1027:1040	similar levels of enzyme secretion and activity	1027:1073	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	11	95	dep	mutants	1829:1835	arg1	efficiencies					1848:1859	' catalytic efficiencies	1836:1859	the other four mutants' catalytic efficiencies	1814:1859	Among them, the two glycomutants which maintained only two N-glycosylation sites each (BxlBN1;5 and BxlBN5;7) showed improved catalytic efficiency, whereas the other four mutants' catalytic efficiencies were reduced.					
31754374	4	96	theme	process	532:538	arg1	understanding					495:507	A greater understanding	485:507	A greater understanding of the N-glycosylation process	485:538	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	12	97	theme	BxlB	1929:1932	arg1	efficiency					1944:1953	improved BxlB catalytic efficiency	1920:1953	improved BxlB catalytic efficiency	1920:1953	The N-glycosylation site N5 is important for improved BxlB catalytic efficiency, but needs to be complemented by N1 and/or N7.					
31754374	15	98	theme	other	2685:2689	arg1	CAZymes					2691:2697	other CAZymes	2685:2697	other CAZymes	2685:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	0	99	theme	N-glycosylation	12:26	arg1	sites					28:32	N-glycosylation sites	12:32	N-glycosylation sites	12:32	Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency.					
31754374	13	100	theme	BxlBnon-glyc	2036:2047	arg1	simulations					2021:2031	Molecular dynamics simulations	2002:2031	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5	2002:2060	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	1	101	theme	BACKGROUND	91:100	arg1	β-Xylosidases					102:114	BACKGROUND β-Xylosidases	91:114	BACKGROUND β-Xylosidases	91:114	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	1	101	theme	BACKGROUND	91:100	arg1	hydrolases					130:139	glycoside hydrolases	120:139	glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose	120:236	BACKGROUND β-Xylosidases are glycoside hydrolases (GHs) that cleave xylooligosaccharides and/or xylobiose into shorter oligosaccharides and xylose.					
31754374	10	102	theme	increased	1638:1646	arg1	activity					1648:1655	its increased activity	1634:1655	its increased activity	1634:1655	Six additional glycomutants were designed based on BxlBN1;5;7, to better understand its increased activity.					
31754374	7	103	theme	similar	1027:1033	arg1	levels					1035:1040	similar levels	1027:1040	similar levels of enzyme secretion and activity	1027:1073	The non-glycosylated mutant (BxlBnon-glyc) showed similar levels of enzyme secretion and activity compared to the wild-type (BxlBwt), while a partially glycosylated mutant (BxlBN1;5;7) exhibited increased activity.					
31754374	4	104	theme	secretion	617:625	arg1	yields					627:632	high secretion yields	612:632	high secretion yields of fungal proteins for industrial applications	612:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	0	105	theme	GH3	39:41	arg1	β-xylosidase					43:54	a GH3 β-xylosidase	37:54	a GH3 β-xylosidase	37:54	Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency.					
31754374	13	106	theme	substrate	2215:2223	arg1	properties					2233:2242	substrate binding properties	2215:2242	substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5	2215:2344	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	15	107	theme	CAZymes	2691:2697	arg1	modification					2669:2680	N-glycosylation modification	2653:2680	N-glycosylation modification of other CAZymes	2653:2697	Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.					
31754374	14	108	theme	thermal	2537:2543	arg1	stability					2545:2553	thermal stability	2537:2553	thermal stability	2537:2553	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	5	109	theme	high	774:777	arg1	activity					779:786	high activity	774:786	high activity	774:786	RESULTS In this study, BxlB-a highly secreted GH3 β-xylosidase from A. nidulans, presenting high activity and several N-glycosylation sites-was selected for N-glycosylation engineering.					
31754374	2	110	theme	Aspergillus	239:249	arg1	model					286:290	an established genetic model	263:290	an established genetic model	263:290	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	2	110	theme	Aspergillus	239:249	arg1	nidulans					251:258	Aspergillus nidulans	239:258	Aspergillus nidulans	239:258	Aspergillus nidulans is an established genetic model and good source of carbohydrate-active enzymes (CAZymes).					
31754374	14	111	theme	BxlB	2431:2434	arg1	production					2436:2445	A. nidulans BxlB production	2419:2445	A. nidulans BxlB production	2419:2445	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	13	112	theme	N5 N-glycosylation	2179:2196	arg1	sites					2198:2202	N5 N-glycosylation sites	2179:2202	N5 N-glycosylation sites	2179:2202	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	4	113	theme	industrial	657:666	arg1	applications					668:679	industrial applications	657:679	industrial applications	657:679	A greater understanding of the N-glycosylation process would contribute to better address the current bottlenecks in obtaining high secretion yields of fungal proteins for industrial applications.					
31754374	14	114	theme	A.	2419:2420	arg1	production					2436:2445	A. nidulans BxlB production	2419:2445	A. nidulans BxlB production	2419:2445	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	13	115	theme	catalytic	2142:2150	arg1	pocket					2152:2157	the catalytic pocket	2138:2157	the catalytic pocket	2138:2157	Molecular dynamics simulations of BxlBnon-glyc and BxlBN1;5 reveals that the mobility pattern of structural elements in the vicinity of the catalytic pocket changes upon N1 and N5 N-glycosylation sites, enhancing substrate binding properties which may underlie the observed differences in catalytic efficiency between BxlBnon-glyc and BxlBN1;5.					
31754374	14	116	theme	promising	2508:2516	arg1	glycoengineering					2486:2501	protein glycoengineering	2478:2501	protein glycoengineering	2478:2501	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	14	116	theme	promising	2508:2516	arg1	tool					2518:2521	a promising tool	2506:2521	a promising tool for enhancing thermal stability, secretion, and enzymatic activity	2506:2588	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
31754374	14	117	theme	N-glycosylation	2400:2414	arg1	influence					2387:2395	the influence	2383:2395	the influence of N-glycosylation on A. nidulans BxlB production and function	2383:2458	CONCLUSIONS This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity.					
30673159	3	0	theme	macromolecules	313:326	arg1	traffic					302:308	traffic	302:308	traffic	302:308	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	4	1	theme	capable	516:522	arg1	sequences					506:514	short peptide sequences	492:514	short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane	492:600	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	4	1	theme	capable	516:522	arg1	peptides					479:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	8	2	theme	zebrafish	1184:1192	arg1	models					1201:1206	zebrafish animal models	1184:1206	zebrafish animal models	1184:1206	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	7	3	theme	penetrating	925:935	arg1	peptides					937:944	penetrating peptides	925:944	penetrating peptides functionalized with glycan residues by an oxime bond connection	925:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	9	4	theme	different	1388:1396	arg1	lines					1403:1407	the different cell lines	1384:1407	the different cell lines tested	1384:1414	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	10	5	theme	peptides	1505:1512	arg1	glycosylation					1476:1488	the glycosylation	1472:1488	the glycosylation of penetrating peptides	1472:1512	The results reported herein highlight the potential of the glycosylation of penetrating peptides to modulate their activity.					
30673159	7	6	theme	oxime	988:992	arg1	connection					999:1008	an oxime bond connection	985:1008	an oxime bond connection	985:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	10	7	theme	glycosylation	1476:1488	arg1	potential					1459:1467	the potential	1455:1467	the potential of the glycosylation of penetrating peptides to modulate their activity	1455:1539	The results reported herein highlight the potential of the glycosylation of penetrating peptides to modulate their activity.					
30673159	8	8	theme	uptake	1015:1020	arg1	efficiency					1022:1031	uptake efficiency	1015:1031	uptake efficiency	1015:1031	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	1	9	theme	cell	52:55	arg1	membrane					57:64	The cell membrane	48:64	The cell membrane	48:64	The cell membrane regulates the exchange of molecules and information with the external environment.					
30673159	9	10	theme	residues	1243:1250	arg1	incorporation					1213:1225	The incorporation	1209:1225	The incorporation of these glycan residues into the peptide structure	1209:1277	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	9	11	theme	cell	1398:1401	arg1	lines					1403:1407	the different cell lines	1384:1407	the different cell lines tested	1384:1414	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	3	12	theme	great	366:370	arg1	challenge					372:380	a great challenge	364:380	a great challenge for the development of the next generation of therapies and diagnostic methods	364:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	4	13	theme	short	492:496	arg1	sequences					506:514	short peptide sequences	492:514	short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane	492:600	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	4	13	theme	short	492:496	arg1	peptides					479:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	7	14	theme	bond	994:997	arg1	connection					999:1008	an oxime bond connection	985:1008	an oxime bond connection	985:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	9	15	dep	efficiency	1310:1319	arg1	the					1290:1292	the	1290:1292	the	1290:1292	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	6	16	theme	materials	836:844	arg1	improvement					811:821	the biological improvement	796:821	the biological improvement of synthetic materials	796:844	Glycosylation has emerged as a potential promising strategy for the biological improvement of synthetic materials.					
30673159	2	17	theme	cellular	264:271	arg1	malfunctions					273:284	cellular malfunctions	264:284	cellular malfunctions	264:284	However, this control barrier hinders the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions.					
30673159	7	18	theme	new	880:882	arg1	strategy					895:902	a new convergent strategy	878:902	a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection	878:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	3	19	theme	diagnostic	442:451	arg1	methods					453:459	diagnostic methods	442:459	diagnostic methods	442:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	0	20	theme	Cell-Penetrating	13:28	arg1	Peptides					30:37	Glycosylated Cell-Penetrating Peptides	0:37	Glycosylated Cell-Penetrating Peptides (GCPPs)	0:45	Glycosylated Cell-Penetrating Peptides (GCPPs).					
30673159	0	20	theme	Cell-Penetrating	13:28	arg1	GCPPs					40:44	GCPPs	40:44	GCPPs	40:44	Glycosylated Cell-Penetrating Peptides (GCPPs).					
30673159	4	21	theme	peptide	498:504	arg1	sequences					506:514	short peptide sequences	492:514	short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane	492:600	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	4	21	theme	peptide	498:504	arg1	peptides					479:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	8	22	theme	flow	1138:1141	arg1	cytometry					1143:1151	flow cytometry	1138:1151	flow cytometry	1138:1151	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	6	23	theme	synthetic	826:834	arg1	materials					836:844	synthetic materials	826:844	synthetic materials	826:844	Glycosylation has emerged as a potential promising strategy for the biological improvement of synthetic materials.					
30673159	7	24	theme	convergent	884:893	arg1	strategy					895:902	a new convergent strategy	878:902	a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection	878:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	0	25	theme	Glycosylated	0:11	arg1	Peptides					30:37	Glycosylated Cell-Penetrating Peptides	0:37	Glycosylated Cell-Penetrating Peptides (GCPPs)	0:45	Glycosylated Cell-Penetrating Peptides (GCPPs).					
30673159	0	25	theme	Glycosylated	0:11	arg1	GCPPs					40:44	GCPPs	40:44	GCPPs	40:44	Glycosylated Cell-Penetrating Peptides (GCPPs).					
30673159	5	26	theme	related	671:677	arg1	limitations					651:661	limitations	651:661	limitations	651:661	However, penetrating peptides still suffer from limitations, mainly related to their lack of specificity and potential toxicity.					
30673159	8	27	theme	glycopeptides	1073:1085	arg1	efficiency					1022:1031	uptake efficiency	1015:1031	uptake efficiency	1015:1031	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	8	27	theme	glycopeptides	1073:1085	arg1	distribution					1051:1062	intracellular distribution	1037:1062	intracellular distribution	1037:1062	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	3	28	theme	therapies	428:436	arg1	generation					414:423	the next generation	405:423	the next generation of therapies and diagnostic methods	405:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	1	29	with	exchange	80:87	arg1	environment					136:146	the external environment	123:146	the external environment	123:146	The cell membrane regulates the exchange of molecules and information with the external environment.					
30673159	9	30	theme	internalization	1294:1308	arg1	efficiency					1310:1319	internalization efficiency	1294:1319	internalization efficiency	1294:1319	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	5	31	theme	penetrating	612:622	arg1	peptides					624:631	penetrating peptides	612:631	penetrating peptides	612:631	However, penetrating peptides still suffer from limitations, mainly related to their lack of specificity and potential toxicity.					
30673159	1	32	theme	molecules	92:100	arg1	exchange					80:87	the exchange	76:87	the exchange of molecules and information with the external environment	76:146	The cell membrane regulates the exchange of molecules and information with the external environment.					
30673159	9	33	theme	peptide	1261:1267	arg1	structure					1269:1277	the peptide structure	1257:1277	the peptide structure	1257:1277	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	8	34	dep	efficiency	1022:1031	arg1	The					1011:1013	The	1011:1013	The	1011:1013	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	3	35	theme	next	409:412	arg1	generation					414:423	the next generation	405:423	the next generation of therapies and diagnostic methods	405:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	4	36	theme	cellular	584:591	arg1	membrane					593:600	the cellular membrane	580:600	the cellular membrane	580:600	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	9	37	theme	cellular	1325:1332	arg1	toxicity					1334:1341	cellular toxicity	1325:1341	cellular toxicity	1325:1341	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	3	38	theme	methods	453:459	arg1	generation					414:423	the next generation	405:423	the next generation of therapies and diagnostic methods	405:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	3	39	theme	generation	414:423	arg1	development					390:400	the development	386:400	the development of the next generation of therapies and diagnostic methods	386:459	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	8	40	theme	intracellular	1037:1049	arg1	distribution					1051:1062	intracellular distribution	1037:1062	intracellular distribution	1037:1062	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	8	41	theme	confocal	1157:1164	arg1	microscopy					1166:1175	confocal microscopy	1157:1175	confocal microscopy	1157:1175	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	2	42	theme	control	163:169	arg1	barrier					171:177	this control barrier	158:177	this control barrier	158:177	However, this control barrier hinders the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions.					
30673159	6	43	theme	biological	800:809	arg1	improvement					811:821	the biological improvement	796:821	the biological improvement of synthetic materials	796:844	Glycosylation has emerged as a potential promising strategy for the biological improvement of synthetic materials.					
30673159	9	44	gly	glycopeptide	1360:1371	arg2	glycopeptide					1360:1371	the resulting glycopeptide hybrids	1346:1379	the resulting glycopeptide hybrids	1346:1379	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	10	45	gly	glycosylation	1476:1488	arg1	peptides					1505:1512	penetrating peptides	1493:1512	penetrating peptides	1493:1512	The results reported herein highlight the potential of the glycosylation of penetrating peptides to modulate their activity.					
30673159	4	46	theme	broad	540:544	arg1	range					546:550	a broad range	538:550	a broad range of biomacromolecules	538:571	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	5	47	theme	specificity	696:706	arg1	lack					688:691	their lack	682:691	their lack of specificity and potential toxicity	682:729	However, penetrating peptides still suffer from limitations, mainly related to their lack of specificity and potential toxicity.					
30673159	4	48	theme	Cell-penetrating	462:477	arg1	sequences					506:514	short peptide sequences	492:514	short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane	492:600	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	4	48	theme	Cell-penetrating	462:477	arg1	peptides					479:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides	462:486	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	2	49	theme	molecules	223:231	arg1	delivery					191:198	the delivery	187:198	the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions	187:284	However, this control barrier hinders the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions.					
30673159	8	50	theme	animal	1194:1199	arg1	models					1201:1206	zebrafish animal models	1184:1206	zebrafish animal models	1184:1206	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	1	51	theme	information	106:116	arg1	exchange					80:87	the exchange	76:87	the exchange of molecules and information with the external environment	76:146	The cell membrane regulates the exchange of molecules and information with the external environment.					
30673159	8	52	gly	glycopeptides	1073:1085	arg2	glycopeptides					1073:1085	these glycopeptides	1067:1085	these glycopeptides	1067:1085	The uptake efficiency and intracellular distribution of these glycopeptides have been systematically characterized by means of flow cytometry and confocal microscopy and in zebrafish animal models.					
30673159	4	53	theme	biomacromolecules	555:571	arg1	range					546:550	a broad range	538:550	a broad range of biomacromolecules	538:571	Cell-penetrating peptides are short peptide sequences capable of delivering a broad range of biomacromolecules across the cellular membrane.					
30673159	2	54	theme	exogenous	203:211	arg1	molecules					223:231	exogenous bioactive molecules	203:231	exogenous bioactive molecules that can be applied to correct cellular malfunctions	203:284	However, this control barrier hinders the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions.					
30673159	2	55	theme	bioactive	213:221	arg1	molecules					223:231	exogenous bioactive molecules	203:231	exogenous bioactive molecules that can be applied to correct cellular malfunctions	203:284	However, this control barrier hinders the delivery of exogenous bioactive molecules that can be applied to correct cellular malfunctions.					
30673159	5	56	theme	potential	712:720	arg1	toxicity					722:729	potential toxicity	712:729	potential toxicity	712:729	However, penetrating peptides still suffer from limitations, mainly related to their lack of specificity and potential toxicity.					
30673159	9	57	theme	resulting	1350:1358	arg1	hybrids					1373:1379	the resulting glycopeptide hybrids	1346:1379	the resulting glycopeptide hybrids	1346:1379	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	10	58	theme	penetrating	1493:1503	arg1	peptides					1505:1512	penetrating peptides	1493:1512	penetrating peptides	1493:1512	The results reported herein highlight the potential of the glycosylation of penetrating peptides to modulate their activity.					
30673159	7	59	theme	glycan	966:971	arg1	residues					973:980	glycan residues	966:980	glycan residues	966:980	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	5	60	theme	toxicity	722:729	arg1	lack					688:691	their lack	682:691	their lack of specificity and potential toxicity	682:729	However, penetrating peptides still suffer from limitations, mainly related to their lack of specificity and potential toxicity.					
30673159	9	61	theme	glycopeptide	1360:1371	arg1	hybrids					1373:1379	the resulting glycopeptide hybrids	1346:1379	the resulting glycopeptide hybrids	1346:1379	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	6	62	theme	promising	773:781	arg1	strategy					783:790	a potential promising strategy	761:790	a potential promising strategy for the biological improvement of synthetic materials	761:844	Glycosylation has emerged as a potential promising strategy for the biological improvement of synthetic materials.					
30673159	1	63	theme	external	127:134	arg1	environment					136:146	the external environment	123:146	the external environment	123:146	The cell membrane regulates the exchange of molecules and information with the external environment.					
30673159	7	64	theme	peptides	937:944	arg1	synthesis					912:920	the synthesis	908:920	the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection	908:1008	In this work we have developed a new convergent strategy for the synthesis of penetrating peptides functionalized with glycan residues by an oxime bond connection.					
30673159	9	65	theme	hybrids	1373:1379	arg1	toxicity					1334:1341	cellular toxicity	1325:1341	cellular toxicity	1325:1341	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	9	65	theme	hybrids	1373:1379	arg1	efficiency					1310:1319	internalization efficiency	1294:1319	internalization efficiency	1294:1319	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
30673159	6	66	theme	potential	763:771	arg1	strategy					783:790	a potential promising strategy	761:790	a potential promising strategy for the biological improvement of synthetic materials	761:844	Glycosylation has emerged as a potential promising strategy for the biological improvement of synthetic materials.					
30673159	3	67	theme	cell	339:342	arg1	membrane					344:351	the cell membrane	335:351	the cell membrane	335:351	Therefore, the traffic of macromolecules across the cell membrane represents a great challenge for the development of the next generation of therapies and diagnostic methods.					
30673159	9	68	theme	glycan	1236:1241	arg1	residues					1243:1250	these glycan residues	1230:1250	these glycan residues	1230:1250	The incorporation of these glycan residues into the peptide structure influenced the internalization efficiency and cellular toxicity of the resulting glycopeptide hybrids in the different cell lines tested.					
31592495	8	0	from	glycans	1221:1227	arg1	residues					1245:1252	residues N311 and N333	1245:1266	residues	1245:1252	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	8	0	from	glycans	1221:1227	arg1	N333					1263:1266	N333	1263:1266	N333	1263:1266	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	8	0	from	glycans	1221:1227	arg1	deficient					1189:1197	deficient	1189:1197	deficient	1189:1197	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	11	1	theme	genes	1753:1757	arg1	category					1716:1723	the largest category	1704:1723	the largest category of differentially expressed genes between HCC tumor and adjacent tissue	1704:1795	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	13	2	theme	cell-surface	2110:2121	arg1	CD73					2123:2126	cell-surface CD73	2110:2126	cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC	2110:2230	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	8	3	theme	hybrid	1202:1207	arg1	glycans					1221:1227	hybrid and complex glycans	1202:1227	glycans	1221:1227	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	6	4	theme	tumor	894:898	arg1	hepatocytes					900:910	tumor hepatocytes	894:910	tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence	894:1025	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	12	5	theme	tumor	1893:1897	arg1	tissue					1899:1904	tumor tissue	1893:1904	tumor tissue	1893:1904	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	8	6	theme	located	1268:1274	arg1	residues					1245:1252	residues N311 and N333	1245:1266	residues	1245:1252	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	11	7	theme	N-linked	1655:1662	arg1	genes					1686:1690	N-linked glycoprotein-encoding genes	1655:1690	N-linked glycoprotein-encoding genes	1655:1690	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	4	8	from	studies	659:665	arg1	cells					674:678	HCC cells	670:678	HCC cells	670:678	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	8	9	theme	complex	1213:1219	arg1	glycans					1221:1227	hybrid and complex glycans	1202:1227	glycans	1221:1227	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	4	10	theme	molecular	553:561	arg1	analyses					590:597	molecular, biochemical, and cellular analyses	553:597	molecular, biochemical, and cellular analyses	553:597	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	4	11	theme	HCC	670:672	arg1	cells					674:678	HCC cells	670:678	HCC cells	670:678	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	13	12	theme	therapeutic	2067:2077	arg1	antibodies					2089:2098	therapeutic candidate antibodies	2067:2098	therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC	2067:2230	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	6	13	theme	etiology	983:990	arg1	independent					964:974	independent	964:974	independent	964:974	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	12	14	theme	glycosylation	1865:1877	arg1	characterization					1840:1855	the first detailed characterization	1821:1855	the first detailed characterization of CD73 glycosylation in normal and tumor tissue	1821:1904	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	8	15	theme	catalytic	1294:1302	arg1	domain					1304:1309	the C-terminal catalytic domain	1279:1309	the C-terminal catalytic domain	1279:1309	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	7	16	theme	3-fold	1090:1095	arg1	decrease					1097:1104	a 3-fold decrease	1088:1104	a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001)	1088:1145	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	1	17	theme	cancer	212:217	arg1	immunotherapy					219:231	cancer immunotherapy	212:231	cancer immunotherapy	212:231	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	12	18	from	characterization	1840:1855	arg1	normal					1882:1887	normal	1882:1887	normal	1882:1887	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	12	18	from	characterization	1840:1855	arg1	tissue					1899:1904	tumor tissue	1893:1904	tumor tissue	1893:1904	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	1	19	gly	glycoprotein	138:149	arg1	glycoprotein					138:149	a cell-surface N-linked glycoprotein	114:149	a cell-surface N-linked glycoprotein that produces extracellular adenosine	114:187	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	1	19	gly	glycoprotein	138:149	arg1	CD73					108:111	CD73	108:111	CD73	108:111	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	12	20	from	suppression	1964:1974	arg1	cells					2003:2007	human HCC tumor cells	1987:2007	human HCC tumor cells	1987:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	7	21	theme	CD73	1071:1074	arg1	glycosylation					1037:1049	Aberrant glycosylation	1028:1049	Aberrant glycosylation of tumor-associated CD73	1028:1074	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	8	22	from	located	1268:1274	arg1	domain					1304:1309	the C-terminal catalytic domain	1279:1309	the C-terminal catalytic domain	1279:1309	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	1	23	theme	N-linked	129:136	arg1	glycoprotein					138:149	a cell-surface N-linked glycoprotein	114:149	a cell-surface N-linked glycoprotein that produces extracellular adenosine	114:187	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	1	23	theme	N-linked	129:136	arg1	CD73					108:111	CD73	108:111	CD73	108:111	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	12	24	theme	first	1825:1829	arg1	characterization					1840:1855	the first detailed characterization	1821:1855	the first detailed characterization of CD73 glycosylation in normal and tumor tissue	1821:1904	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	6	25	theme	N-linked	940:947	arg1	glycosylation					949:961	abnormal N-linked glycosylation	931:961	abnormal N-linked glycosylation	931:961	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	9	26	with	CD73	1383:1386	arg1	activity					1433:1440	significantly decreased 5'-nucleotidase activity	1393:1440	significantly decreased 5'-nucleotidase activity	1393:1440	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	6	27	link	N-linked	940:947	arg1	glycosylation					949:961	abnormal N-linked glycosylation	931:961	abnormal N-linked glycosylation	931:961	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	13	28	theme	translational	2036:2048	arg1	implications					2050:2061	translational implications	2036:2061	translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC	2036:2230	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	5	29	theme	other	763:767	arg1	glycoproteins					778:790	other N-linked glycoproteins	763:790	other N-linked glycoproteins	763:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	9	30	theme	Blocking	1312:1319	arg1	glycosylation					1331:1343	Blocking N311/N333 glycosylation	1312:1343	Blocking N311/N333 glycosylation by site-directed mutagenesis	1312:1372	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	1	31	theme	extracellular	165:177	arg1	adenosine					179:187	extracellular adenosine	165:187	extracellular adenosine	165:187	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	6	32	theme	fibrosis	1009:1016	arg1	presence					1018:1025	fibrosis presence	1009:1025	fibrosis presence	1009:1025	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	0	33	from	Glycosylation	24:36	arg1	Carcinoma					97:105	Human Hepatocellular Carcinoma	76:105	Human Hepatocellular Carcinoma	76:105	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	0	34	theme	Hepatocellular	82:95	arg1	Carcinoma					97:105	Human Hepatocellular Carcinoma	76:105	Human Hepatocellular Carcinoma	76:105	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	9	35	theme	site-directed	1348:1360	arg1	mutagenesis					1362:1372	site-directed mutagenesis	1348:1372	site-directed mutagenesis	1348:1372	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	6	36	theme	tumor	993:997	arg1	stage					999:1003	tumor stage	993:1003	tumor stage	993:1003	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	5	37	gly	glycoproteins	778:790	arg1	glycoproteins					778:790	other N-linked glycoproteins	763:790	other N-linked glycoproteins	763:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	12	38	theme	tumor	1997:2001	arg1	cells					2003:2007	human HCC tumor cells	1987:2007	human HCC tumor cells	1987:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	10	39	theme	structural	1549:1558	arg1	GM130					1568:1572	the Golgi structural protein GM130	1539:1572	the Golgi structural protein GM130	1539:1572	Glycosylation-deficient CD73 partially colocalized with the Golgi structural protein GM130, which was strongly induced in HCC tumors.					
31592495	0	40	theme	Tumor-Selective	0:14	arg1	Glycosylation					24:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	9	41	theme	decreased	1407:1415	arg1	activity					1433:1440	significantly decreased 5'-nucleotidase activity	1393:1440	significantly decreased 5'-nucleotidase activity	1393:1440	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	12	42	theme	CD73	1979:1982	arg1	suppression					1964:1974	the functional suppression	1949:1974	the functional suppression of CD73 in human HCC tumor cells	1949:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	3	43	theme	human	453:457	arg1	HCC					485:487	HCC	485:487	HCC	485:487	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	3	43	theme	human	453:457	arg1	carcinoma					474:482	human hepatocellular carcinoma	453:482	human hepatocellular carcinoma (HCC)	453:488	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	0	44	theme	Functional	42:51	arg1	Attenuation					53:63	Functional Attenuation	42:63	Functional Attenuation	42:63	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	12	45	theme	human	1987:1991	arg1	cells					2003:2007	human HCC tumor cells	1987:2007	human HCC tumor cells	1987:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	11	46	theme	largest	1708:1714	arg1	category					1716:1723	the largest category	1704:1723	the largest category of differentially expressed genes between HCC tumor and adjacent tissue	1704:1795	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	2	47	theme	target	364:369	arg1	cell					371:374	the target cell and tumor type	360:389	cell	371:374	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	8	48	from	deficient	1189:1197	arg1	glycans					1221:1227	hybrid and complex glycans	1202:1227	glycans	1221:1227	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	13	49	from	CD73	2123:2126	arg1	tumors					2137:2142	solid tumors	2131:2142	solid tumors	2131:2142	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	13	49	from	CD73	2123:2126	arg1	agonists					2182:2189	small-molecule adenosine receptor agonists	2148:2189	small-molecule adenosine receptor agonists	2148:2189	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	11	50	theme	expressed	1743:1751	arg1	genes					1753:1757	differentially expressed genes	1728:1757	differentially expressed genes	1728:1757	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	11	51	link	N-linked	1655:1662	arg1	genes					1686:1690	N-linked glycoprotein-encoding genes	1655:1690	N-linked glycoprotein-encoding genes	1655:1690	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	5	52	theme	mass	801:804	arg1	spectrometry					806:817	mass spectrometry glycomics and RNA sequencing (RNAseq)	801:855	spectrometry	806:817	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	6	53	theme	abnormal	931:938	arg1	glycosylation					949:961	abnormal N-linked glycosylation	931:961	abnormal N-linked glycosylation	931:961	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	12	54	theme	novel	1919:1923	arg1	mechanism					1925:1933	a novel mechanism	1917:1933	a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells	1917:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	4	55	theme	HCC	610:612	arg1	specimens					623:631	primary HCC surgical specimens	602:631	primary HCC surgical specimens	602:631	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	5	56	from	signatures	705:714	arg1	transcripts					742:752	transcripts	742:752	transcripts encoding other N-linked glycoproteins	742:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	8	57	theme	tumor-associated	1163:1178	arg1	CD73					1180:1183	tumor-associated CD73	1163:1183	tumor-associated CD73	1163:1183	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	11	58	theme	glycoprotein-encoding	1664:1684	arg1	genes					1686:1690	N-linked glycoprotein-encoding genes	1655:1690	N-linked glycoprotein-encoding genes	1655:1690	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	2	59	theme	protumor	313:320	arg1	functions					336:344	both protumor and antitumor functions	308:344	both protumor and antitumor functions	308:344	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	13	60	theme	adenosine	2163:2171	arg1	agonists					2182:2189	small-molecule adenosine receptor agonists	2148:2189	small-molecule adenosine receptor agonists	2148:2189	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	4	61	theme	cellular	581:588	arg1	analyses					590:597	molecular, biochemical, and cellular analyses	553:597	molecular, biochemical, and cellular analyses	553:597	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	7	62	theme	5'-nucleotidase	1109:1123	arg1	P < 0.0001					1135:1144	P < 0.0001	1135:1144	P < 0.0001	1135:1144	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	7	62	theme	5'-nucleotidase	1109:1123	arg1	activity					1125:1132	5'-nucleotidase activity	1109:1132	5'-nucleotidase activity (P < 0.0001)	1109:1145	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	11	63	theme	RNAseq	1617:1622	arg1	analysis					1624:1631	RNAseq analysis	1617:1631	RNAseq analysis	1617:1631	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	5	64	dep	spectrometry	806:817	arg1	glycomics					819:827	glycomics	819:827	glycomics	819:827	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	12	65	theme	CD73	1860:1863	arg1	glycosylation					1865:1877	CD73 glycosylation	1860:1877	CD73 glycosylation	1860:1877	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	4	66	theme	mechanistic	647:657	arg1	studies					659:665	mechanistic studies	647:665	mechanistic studies in HCC cells	647:678	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	11	67	theme	adjacent	1781:1788	arg1	tissue					1790:1795	HCC tumor and adjacent tissue	1767:1795	tissue	1790:1795	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	10	68	theme	Glycosylation-deficient	1483:1505	arg1	CD73					1507:1510	Glycosylation-deficient CD73	1483:1510	Glycosylation-deficient CD73	1483:1510	Glycosylation-deficient CD73 partially colocalized with the Golgi structural protein GM130, which was strongly induced in HCC tumors.					
31592495	5	69	link	N-linked	769:776	arg1	glycoproteins					778:790	other N-linked glycoproteins	763:790	other N-linked glycoproteins	763:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	1	70	theme	novel	195:199	arg1	CD73					108:111	CD73	108:111	CD73	108:111	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	1	70	theme	novel	195:199	arg1	target					201:206	a novel target	193:206	a novel target for cancer immunotherapy	193:231	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	5	71	theme	CD73	693:696	arg1	signatures					705:714	CD73 glycan signatures	693:714	CD73 glycan signatures	693:714	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	13	72	theme	candidate	2079:2087	arg1	antibodies					2089:2098	therapeutic candidate antibodies	2067:2098	therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC	2067:2230	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	2	73	theme	antitumor	326:334	arg1	functions					336:344	both protumor and antitumor functions	308:344	both protumor and antitumor functions	308:344	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	6	74	theme	HCC	979:981	arg1	etiology					983:990	HCC etiology	979:990	HCC etiology	979:990	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	8	75	theme	C-terminal	1283:1292	arg1	domain					1304:1309	the C-terminal catalytic domain	1279:1309	the C-terminal catalytic domain	1279:1309	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	7	76	theme	tumor-associated	1054:1069	arg1	CD73					1071:1074	tumor-associated CD73	1054:1074	tumor-associated CD73	1054:1074	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	5	77	from	alterations	727:737	arg1	transcripts					742:752	transcripts	742:752	transcripts encoding other N-linked glycoproteins	742:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	7	78	theme	Aberrant	1028:1035	arg1	glycosylation					1037:1049	Aberrant glycosylation	1028:1049	Aberrant glycosylation of tumor-associated CD73	1028:1074	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	1	79	theme	cell-surface	116:127	arg1	glycoprotein					138:149	a cell-surface N-linked glycoprotein	114:149	a cell-surface N-linked glycoprotein that produces extracellular adenosine	114:187	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	1	79	theme	cell-surface	116:127	arg1	CD73					108:111	CD73	108:111	CD73	108:111	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	13	80	theme	present	2014:2020	arg1	findings					2022:2029	The present findings	2010:2029	The present findings	2010:2029	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	7	81	gly	glycosylation	1037:1049	arg1	CD73					1071:1074	tumor-associated CD73	1054:1074	tumor-associated CD73	1054:1074	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	0	82	theme	Human	76:80	arg1	Carcinoma					97:105	Human Hepatocellular Carcinoma	76:105	Human Hepatocellular Carcinoma	76:105	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	2	83	theme	clinical	277:284	arg1	development					286:296	clinical development	277:296	clinical development	277:296	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	8	84	from	domain	1304:1309	arg1	located					1268:1274	located	1268:1274	located	1268:1274	Biochemically, tumor-associated CD73 was deficient in hybrid and complex glycans specifically on residues N311 and N333 located in the C-terminal catalytic domain.					
31592495	12	85	theme	detailed	1831:1838	arg1	characterization					1840:1855	the first detailed characterization	1821:1855	the first detailed characterization of CD73 glycosylation in normal and tumor tissue	1821:1904	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	6	86	theme	presence	1018:1025	arg1	independent					964:974	independent	964:974	independent	964:974	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	4	87	theme	CD73	503:506	arg1	expression					508:517	CD73 expression	503:517	CD73 expression	503:517	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	5	88	theme	N-linked	769:776	arg1	glycoproteins					778:790	other N-linked glycoproteins	763:790	other N-linked glycoproteins	763:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	9	89	theme	N311/N333	1321:1329	arg1	glycosylation					1331:1343	Blocking N311/N333 glycosylation	1312:1343	Blocking N311/N333 glycosylation by site-directed mutagenesis	1312:1372	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	5	90	theme	glycan	698:703	arg1	signatures					705:714	CD73 glycan signatures	693:714	CD73 glycan signatures	693:714	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	5	91	theme	global	720:725	arg1	alterations					727:737	global alterations	720:737	global alterations in transcripts encoding other N-linked glycoproteins	720:790	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	6	92	theme	stage	999:1003	arg1	independent					964:974	independent	964:974	independent	964:974	CD73 was expressed on tumor hepatocytes where it exhibited abnormal N-linked glycosylation, independent of HCC etiology, tumor stage, or fibrosis presence.					
31592495	0	93	from	Attenuation	53:63	arg1	Carcinoma					97:105	Human Hepatocellular Carcinoma	76:105	Human Hepatocellular Carcinoma	76:105	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	10	94	theme	protein	1560:1566	arg1	GM130					1568:1572	the Golgi structural protein GM130	1539:1572	the Golgi structural protein GM130	1539:1572	Glycosylation-deficient CD73 partially colocalized with the Golgi structural protein GM130, which was strongly induced in HCC tumors.					
31592495	0	95	theme	Altered	16:22	arg1	Glycosylation					24:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	13	96	theme	clinical	2203:2210	arg1	development					2212:2222	clinical development	2203:2222	clinical development	2203:2222	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	7	97	from	decrease	1097:1104	arg1	P < 0.0001					1135:1144	P < 0.0001	1135:1144	P < 0.0001	1135:1144	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	7	97	from	decrease	1097:1104	arg1	activity					1125:1132	5'-nucleotidase activity	1109:1132	5'-nucleotidase activity (P < 0.0001)	1109:1145	Aberrant glycosylation of tumor-associated CD73 resulted in a 3-fold decrease in 5'-nucleotidase activity (P < 0.0001).					
31592495	10	98	theme	Golgi	1543:1547	arg1	GM130					1568:1572	the Golgi structural protein GM130	1539:1572	the Golgi structural protein GM130	1539:1572	Glycosylation-deficient CD73 partially colocalized with the Golgi structural protein GM130, which was strongly induced in HCC tumors.					
31592495	3	99	theme	CD73	434:437	arg1	regulation					439:448	CD73 regulation	434:448	CD73 regulation in human hepatocellular carcinoma (HCC)	434:488	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	5	100	theme	RNA	833:835	arg1	RNAseq					849:854	RNAseq	849:854	RNAseq	849:854	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	5	100	theme	RNA	833:835	arg1	sequencing					837:846	RNA sequencing	833:846	RNA sequencing (RNAseq)	833:855	We analyzed CD73 glycan signatures and global alterations in transcripts encoding other N-linked glycoproteins by using mass spectrometry glycomics and RNA sequencing (RNAseq), respectively.					
31592495	2	101	contain	has	304:306	arg1	CD73					299:302	CD73	299:302	CD73	299:302	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	2	101	contain	has	304:306	arg2	functions					336:344	both protumor and antitumor functions	308:344	both protumor and antitumor functions	308:344	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	13	102	theme	solid	2131:2135	arg1	tumors					2137:2142	solid tumors	2131:2142	solid tumors	2131:2142	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	4	103	theme	biochemical	564:574	arg1	analyses					590:597	molecular, biochemical, and cellular analyses	553:597	molecular, biochemical, and cellular analyses	553:597	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	9	104	theme	5'-nucleotidase	1417:1431	arg1	activity					1433:1440	significantly decreased 5'-nucleotidase activity	1393:1440	significantly decreased 5'-nucleotidase activity	1393:1440	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31592495	12	105	theme	HCC	1993:1995	arg1	cells					2003:2007	human HCC tumor cells	1987:2007	human HCC tumor cells	1987:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	13	106	theme	small-molecule	2148:2161	arg1	agonists					2182:2189	small-molecule adenosine receptor agonists	2148:2189	small-molecule adenosine receptor agonists	2148:2189	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	3	107	theme	hepatocellular	459:472	arg1	HCC					485:487	HCC	485:487	HCC	485:487	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	3	107	theme	hepatocellular	459:472	arg1	carcinoma					474:482	human hepatocellular carcinoma	453:482	human hepatocellular carcinoma (HCC)	453:488	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	0	108	theme	CD73	68:71	arg1	Glycosylation					24:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation	0:36	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	0	108	theme	CD73	68:71	arg1	Attenuation					53:63	Functional Attenuation	42:63	Functional Attenuation	42:63	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	12	109	theme	functional	1953:1962	arg1	suppression					1964:1974	the functional suppression	1949:1974	the functional suppression of CD73 in human HCC tumor cells	1949:2007	Conclusion: We provide the first detailed characterization of CD73 glycosylation in normal and tumor tissue, revealing a novel mechanism that leads to the functional suppression of CD73 in human HCC tumor cells.					
31592495	0	110	gly	Glycosylation	24:36	arg1	CD73					68:71	CD73	68:71	CD73	68:71	Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular Carcinoma.					
31592495	3	111	theme	study	408:412	arg1	aim					396:398	The aim	392:398	The aim of this study	392:412	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	10	112	theme	HCC	1605:1607	arg1	tumors					1609:1614	HCC tumors	1605:1614	HCC tumors	1605:1614	Glycosylation-deficient CD73 partially colocalized with the Golgi structural protein GM130, which was strongly induced in HCC tumors.					
31592495	13	113	theme	receptor	2173:2180	arg1	agonists					2182:2189	small-molecule adenosine receptor agonists	2148:2189	small-molecule adenosine receptor agonists	2148:2189	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	13	114	contain	have	2031:2034	arg1	findings					2022:2029	The present findings	2010:2029	The present findings	2010:2029	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	13	114	contain	have	2031:2034	arg2	implications					2050:2061	translational implications	2036:2061	translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC	2036:2230	The present findings have translational implications for therapeutic candidate antibodies targeting cell-surface CD73 in solid tumors and small-molecule adenosine receptor agonists that are in clinical development for HCC.					
31592495	4	115	theme	primary	602:608	arg1	specimens					623:631	primary HCC surgical specimens	602:631	primary HCC surgical specimens	602:631	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	1	116	link	N-linked	129:136	arg1	glycoprotein					138:149	a cell-surface N-linked glycoprotein	114:149	a cell-surface N-linked glycoprotein that produces extracellular adenosine	114:187	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	1	116	link	N-linked	129:136	arg1	CD73					108:111	CD73	108:111	CD73	108:111	CD73, a cell-surface N-linked glycoprotein that produces extracellular adenosine, is a novel target for cancer immunotherapy.					
31592495	4	117	theme	surgical	614:621	arg1	specimens					623:631	primary HCC surgical specimens	602:631	primary HCC surgical specimens	602:631	We examined CD73 expression, localization, and activity using molecular, biochemical, and cellular analyses on primary HCC surgical specimens, coupled with mechanistic studies in HCC cells.					
31592495	11	118	theme	HCC	1767:1769	arg1	tumor					1771:1775	HCC tumor and adjacent tissue	1767:1795	tumor	1771:1775	RNAseq analysis further revealed that N-linked glycoprotein-encoding genes represented the largest category of differentially expressed genes between HCC tumor and adjacent tissue.					
31592495	3	119	from	regulation	439:448	arg1	HCC					485:487	HCC	485:487	HCC	485:487	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	3	119	from	regulation	439:448	arg1	carcinoma					474:482	human hepatocellular carcinoma	453:482	human hepatocellular carcinoma (HCC)	453:488	The aim of this study was to characterize CD73 regulation in human hepatocellular carcinoma (HCC).					
31592495	2	120	theme	tumor	380:384	arg1	type					386:389	the target cell and tumor type	360:389	type	386:389	Although anti-CD73 antibodies have entered clinical development, CD73 has both protumor and antitumor functions, depending on the target cell and tumor type.					
31592495	9	121	theme	primary	1467:1473	arg1	tumors					1475:1480	the primary tumors	1463:1480	the primary tumors	1463:1480	Blocking N311/N333 glycosylation by site-directed mutagenesis produced CD73 with significantly decreased 5'-nucleotidase activity in vitro, similar to the primary tumors.					
31597244	5	0	theme	structural	688:697	arg1	changes					699:705	structural changes	688:705	structural changes related to Steam Explosion	688:732	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	1	1	from	change	191:196	arg1	composition					211:221	the ionic composition	201:221	the ionic composition on the surface of the sugar cane bagasse	201:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	1	1	from	change	191:196	arg1	surface					230:236	the surface	226:236	the surface of the sugar cane bagasse	226:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	3	2	theme	Microwave	511:519	arg1	pretreatments					527:539	Microwave:H2SO4 pretreatments	511:539	Microwave:H2SO4 pretreatments	511:539	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	1	3	theme	cane	251:254	arg1	bagasse					256:262	the sugar cane bagasse	241:262	the sugar cane bagasse	241:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	5	4	theme	surface-bounded	748:762	arg1	Li+					764:766	released surface-bounded Li+	739:766	released surface-bounded Li+	739:766	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	6	5	theme	relative	858:865	arg1	concentration					867:879	relative concentration	858:879	relative concentration	858:879	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	11	6	theme	enzymatic	1539:1547	arg1	activities					1549:1558	the enzymatic activities	1535:1558	the enzymatic activities	1535:1558	However, there were correlations between the Li+ distribution patterns and the enzymatic activities that should to be shown.					
31597244	1	7	theme	bagasse	256:262	arg1	surface					230:236	the surface	226:236	the surface of the sugar cane bagasse	226:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	8	8	from	pattern	1207:1213	arg1	substrate					1222:1230	the substrate	1218:1230	the substrate	1218:1230	Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification.					
31597244	5	9	theme	related	707:713	arg1	changes					699:705	structural changes	688:705	structural changes related to Steam Explosion	688:732	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	3	10	theme	H2SO4	521:525	arg1	pretreatments					527:539	Microwave:H2SO4 pretreatments	511:539	Microwave:H2SO4 pretreatments	511:539	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	3	11	theme	structural	560:569	arg1	changes					571:577	unrelated structural changes	550:577	unrelated structural changes	550:577	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	9	12	theme	enzyme	1327:1332	arg1	activity					1334:1341	enzyme activity	1327:1341	enzyme activity	1327:1341	Apparently, it acts as a phase-dependent modulator of enzyme activity.					
31597244	0	13	theme	Hydrolytic	94:103	arg1	Saccharification					105:120	the Hydrolytic Saccharification	90:120	the Hydrolytic Saccharification of Sugarcane Bagasse	90:141	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	9	14	theme	activity	1334:1341	arg1	modulator					1314:1322	a phase-dependent modulator	1296:1322	a phase-dependent modulator of enzyme activity	1296:1341	Apparently, it acts as a phase-dependent modulator of enzyme activity.					
31597244	9	14	theme	activity	1334:1341	arg1	it					1285:1286	it	1285:1286	it	1285:1286	Apparently, it acts as a phase-dependent modulator of enzyme activity.					
31597244	7	15	from	decrease	1102:1109	arg1	yields					1131:1136	saccharification yields	1114:1136	saccharification yields	1114:1136	These changes correlated to 1.90-fold decrease in saccharification yields.					
31597244	0	16	theme	Sugarcane	125:133	arg1	Bagasse					135:141	Sugarcane Bagasse	125:141	Sugarcane Bagasse	125:141	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	8	17	theme	distribution	1194:1205	arg1	pattern					1207:1213	distribution pattern	1194:1213	distribution pattern	1194:1213	Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification.					
31597244	6	18	theme	higher	840:845	arg1	amounts					847:853	the higher amounts	836:853	the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol	836:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	1	19	theme	soft	186:189	arg1	change					191:196	the very soft change	177:196	the very soft change in the ionic composition on the surface of the sugar cane bagasse	177:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	8	20	from	activator	1150:1158	arg1	solution					1163:1170	solution	1163:1170	solution	1163:1170	Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification.					
31597244	3	21	theme	unrelated	550:558	arg1	changes					571:577	unrelated structural changes	550:577	unrelated structural changes	550:577	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	5	22	theme	reducing	793:800	arg1	sugars					802:807	2.04-fold more reducing sugars	778:807	2.04-fold more reducing sugars than the control	778:824	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	0	23	theme	Discriminant	7:18	arg1	Analysis					20:27	Matrix Discriminant Analysis	0:27	Matrix Discriminant Analysis	0:27	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	3	24	theme	similar	584:590	arg1	patterns					611:618	similar ionic distribution patterns	584:618	similar ionic distribution patterns	584:618	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	0	25	theme	Matrix	0:5	arg1	Analysis					20:27	Matrix Discriminant Analysis	0:27	Matrix Discriminant Analysis	0:27	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	7	26	theme	saccharification	1114:1129	arg1	yields					1131:1136	saccharification yields	1114:1136	saccharification yields	1114:1136	These changes correlated to 1.90-fold decrease in saccharification yields.					
31597244	10	27	theme	cocktail	1445:1452	arg1	changes					1401:1407	structural changes	1390:1407	structural changes	1390:1407	Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used.					
31597244	10	27	theme	cocktail	1445:1452	arg1	efficiency					1417:1426	the efficiency	1413:1426	the efficiency of the enzymatic cocktail used	1413:1457	Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used.					
31597244	3	28	theme	ionic	592:596	arg1	patterns					611:618	similar ionic distribution patterns	584:618	similar ionic distribution patterns	584:618	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	6	29	dep	amounts	847:853	arg1	DMSO					990:993	DMSO	990:993	DMSO	990:993	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	29	dep	amounts	847:853	arg1	Oxalate					1004:1010	Ammonium Oxalate	995:1010	Ammonium Oxalate	995:1010	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	30	located	observed	927:934	arg2	surface					919:925	the surface	915:925	the surface observed in the control	915:949	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	30	located	observed	927:934	arg1	control					943:949	the control	939:949	the control	939:949	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	31	theme	Ammonium	995:1002	arg1	Oxalate					1004:1010	Ammonium Oxalate	995:1010	Ammonium Oxalate	995:1010	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	5	32	theme	released	739:746	arg1	Li+					764:766	released surface-bounded Li+	739:766	released surface-bounded Li+	739:766	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	10	33	theme	enzymatic	1435:1443	arg1	cocktail					1445:1452	the enzymatic cocktail	1431:1452	the enzymatic cocktail used	1431:1457	Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used.					
31597244	11	34	theme	Li+	1505:1507	arg1	patterns					1522:1529	the Li+ distribution patterns	1501:1529	the Li+ distribution patterns	1501:1529	However, there were correlations between the Li+ distribution patterns and the enzymatic activities that should to be shown.					
31597244	1	35	theme	ionic	205:209	arg1	composition					211:221	the ionic composition	201:221	the ionic composition on the surface of the sugar cane bagasse	201:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	6	36	with	pretreatment	964:975	arg1	Ethanol					982:988	Ethanol	982:988	Ethanol	982:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	0	37	theme	Bagasse	135:141	arg1	Saccharification					105:120	the Hydrolytic Saccharification	90:120	the Hydrolytic Saccharification of Sugarcane Bagasse	90:141	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	2	38	theme	related	416:422	arg1	factor					409:414	the most important factor	390:414	the most important factor related to the increase of saccharification yields using enzyme cocktails	390:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	2	38	theme	related	416:422	arg1	Removal					335:341	Removal	335:341	Removal of Li+ by pretreatments exposing -OH sites	335:384	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	6	39	from	surface	919:925	arg1	amounts					847:853	the higher amounts	836:853	the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol	836:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	2	40	theme	enzyme	473:478	arg1	cocktails					480:488	enzyme cocktails	473:488	enzyme cocktails	473:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	1	41	theme	sugars	296:301	arg1	yields					286:291	yields	286:291	yields of sugars	286:301	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	6	42	from	concentration	867:879	arg1	surface					919:925	the surface	915:925	the surface observed in the control	915:949	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	5	43	theme	more	788:791	arg1	sugars					802:807	2.04-fold more reducing sugars	778:807	2.04-fold more reducing sugars than the control	778:824	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	6	44	theme	available	1032:1040	arg1	-OH					1021:1023	-OH	1021:1023	-OH	1021:1023	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	2	45	theme	important	399:407	arg1	factor					409:414	the most important factor	390:414	the most important factor related to the increase of saccharification yields using enzyme cocktails	390:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	2	45	theme	important	399:407	arg1	Removal					335:341	Removal	335:341	Removal of Li+ by pretreatments exposing -OH sites	335:384	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	0	46	theme	Important	61:69	arg1	Factor					71:76	an Important Factor	58:76	an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse	58:141	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	0	46	theme	Important	61:69	arg1	Surface-Lithium					39:53	Surface-Lithium	39:53	Surface-Lithium	39:53	Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse.					
31597244	5	47	theme	Steam	718:722	arg1	Explosion					724:732	Steam Explosion	718:732	Steam Explosion	718:732	NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control.					
31597244	3	48	theme	distribution	598:609	arg1	patterns					611:618	similar ionic distribution patterns	584:618	similar ionic distribution patterns	584:618	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	2	49	theme	Li+	346:348	arg1	factor					409:414	the most important factor	390:414	the most important factor related to the increase of saccharification yields using enzyme cocktails	390:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	2	49	theme	Li+	346:348	arg1	Removal					335:341	Removal	335:341	Removal of Li+ by pretreatments exposing -OH sites	335:384	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	2	50	theme	yields	460:465	arg1	increase					431:438	the increase	427:438	the increase of saccharification yields using enzyme cocktails	427:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	1	51	theme	hydrolytic	306:315	arg1	saccharification					317:332	hydrolytic saccharification	306:332	hydrolytic saccharification	306:332	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	7	52	theme	1.90-fold	1092:1100	arg1	decrease					1102:1109	1.90-fold decrease	1092:1109	1.90-fold decrease in saccharification yields	1092:1136	These changes correlated to 1.90-fold decrease in saccharification yields.					
31597244	6	53	from	amounts	847:853	arg1	surface					919:925	the surface	915:925	the surface observed in the control	915:949	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	53	from	amounts	847:853	arg1	structures					894:903	periodic structures	885:903	periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol	885:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	53	from	amounts	847:853	arg1	concentration					867:879	relative concentration	858:879	relative concentration	858:879	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	54	theme	Li+	908:910	arg1	concentration					867:879	relative concentration	858:879	relative concentration	858:879	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	6	54	theme	Li+	908:910	arg1	structures					894:903	periodic structures	885:903	periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol	885:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	1	55	from	surface	230:236	arg1	change					191:196	the very soft change	177:196	the very soft change in the ionic composition on the surface of the sugar cane bagasse	177:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	6	56	theme	periodic	885:892	arg1	structures					894:903	periodic structures	885:903	periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol	885:988	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	1	57	from	composition	211:221	arg1	surface					230:236	the surface	226:236	the surface of the sugar cane bagasse	226:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	1	58	theme	Statistical	144:154	arg1	evidence					156:163	Statistical evidence	144:163	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse	144:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
31597244	6	59	theme	ionic	1046:1050	arg1	sputtering					1052:1061	ionic sputtering	1046:1061	ionic sputtering	1046:1061	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	8	60	from	presence	1181:1188	arg1	substrate					1222:1230	the substrate	1218:1230	the substrate	1218:1230	Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification.					
31597244	3	61	theme	Steam	491:495	arg1	Explosion					497:505	Steam Explosion	491:505	Steam Explosion	491:505	Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns.					
31597244	11	62	theme	distribution	1509:1520	arg1	patterns					1522:1529	the Li+ distribution patterns	1501:1529	the Li+ distribution patterns	1501:1529	However, there were correlations between the Li+ distribution patterns and the enzymatic activities that should to be shown.					
31597244	2	63	theme	-OH	376:378	arg1	sites					380:384	-OH sites	376:384	-OH sites	376:384	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	4	64	theme	yield	657:661	arg1	1.74-fold					663:671	the saccharification yield 1.74-fold	636:671	the saccharification yield 1.74-fold	636:671	Both increased the saccharification yield 1.74-fold.					
31597244	6	65	from	structures	894:903	arg1	surface					919:925	the surface	915:925	the surface observed in the control	915:949	In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering.					
31597244	9	66	theme	phase-dependent	1298:1312	arg1	modulator					1314:1322	a phase-dependent modulator	1296:1322	a phase-dependent modulator of enzyme activity	1296:1341	Apparently, it acts as a phase-dependent modulator of enzyme activity.					
31597244	9	66	theme	phase-dependent	1298:1312	arg1	it					1285:1286	it	1285:1286	it	1285:1286	Apparently, it acts as a phase-dependent modulator of enzyme activity.					
31597244	4	67	theme	saccharification	640:655	arg1	1.74-fold					663:671	the saccharification yield 1.74-fold	636:671	the saccharification yield 1.74-fold	636:671	Both increased the saccharification yield 1.74-fold.					
31597244	10	68	theme	structural	1390:1399	arg1	changes					1401:1407	structural changes	1390:1407	structural changes	1390:1407	Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used.					
31597244	2	69	theme	saccharification	443:458	arg1	yields					460:465	saccharification yields	443:465	saccharification yields using enzyme cocktails	443:488	Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails.					
31597244	1	70	theme	sugar	245:249	arg1	bagasse					256:262	the sugar cane bagasse	241:262	the sugar cane bagasse	241:262	Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification.					
29852377	1	0	theme	transitions	184:194	arg1	formation					160:168	the formation	156:168	the formation of structural transitions in tissue that are key to physiological function	156:243	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	1	1	from	transitions	184:194	arg1	tissue					199:204	tissue	199:204	tissue	199:204	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	3	2	theme	growth	547:552	arg1	factors					554:560	growth factors	547:560	growth factors	547:560	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	5	3	theme	mineralized	1066:1076	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	4	from	Gradients	633:641	arg1	hydrogels					686:694	agarose hydrogels	678:694	agarose hydrogels	678:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	4	5	theme	clear	840:844	arg1	transition					854:863	a clear mineral transition	838:863	a clear mineral transition from bone to cartilage	838:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	3	6	theme	biomaterial	612:622	arg1	systems					624:630	biomaterial systems	612:630	biomaterial systems	612:630	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	3	7	theme	biochemical	574:584	arg1	gradients					586:594	biochemical gradients	574:594	biochemical gradients	574:594	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	4	8	theme	robust	788:793	arg1	constructs					816:825	robust osteochondral tissue constructs	788:825	robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage	788:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	5	9	theme	tidemark	1034:1041	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	10	theme	transition	1043:1052	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	11	theme	tissue	809:814	arg1	constructs					816:825	robust osteochondral tissue constructs	788:825	robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage	788:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	3	12	theme	nanoparticles	516:528	arg1	alignment					461:469	magnetic field alignment	446:469	magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors,	446:561	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	4	13	theme	mesenchymal	752:762	arg1	cells					769:773	human mesenchymal stem cells	746:773	human mesenchymal stem cells	746:773	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	3	14	theme	iron	505:508	arg1	nanoparticles					516:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	1	15	theme	developmental	95:107	arg1	biology					109:115	developmental biology	95:115	developmental biology	95:115	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	6	16	theme	platform	1176:1183	arg1	technology					1185:1194	This platform technology	1171:1194	This platform technology	1171:1194	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	3	17	theme	field	455:459	arg1	alignment					461:469	magnetic field alignment	446:469	magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors,	446:561	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	3	18	theme	rapid	421:425	arg1	platform					427:434	a facile and rapid platform	408:434	a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems	408:630	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	6	19	theme	exciting	1237:1244	arg1	opportunity					1250:1260	an exciting new opportunity	1234:1260	an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges	1234:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	2	20	dep	Failure	246:252	arg1	reproduce					257:265	reproduce	257:265	to reproduce these native features in an in vitro setting	254:310	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	2	21	theme	in vitro	295:302	arg1	setting					304:310	an in vitro setting	292:310	an in vitro setting	292:310	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	6	22	theme	great	1203:1207	arg1	versatility					1209:1219	great versatility	1203:1219	great versatility	1203:1219	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	0	23	theme	superparamagnetic	13:29	arg1	gradients					44:52	superparamagnetic nanoparticle gradients	13:52	superparamagnetic nanoparticle gradients for osteochondral tissue engineering	13:89	Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.					
29852377	1	24	from	formation	160:168	arg1	tissue					199:204	tissue	199:204	tissue	199:204	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	5	25	theme	growth	928:933	arg1	factor					935:940	growth factor	928:940	growth factor concentration	928:954	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	3	26	theme	facile	410:415	arg1	platform					427:434	a facile and rapid platform	408:434	a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems	408:630	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	4	27	theme	stem	764:767	arg1	cells					769:773	human mesenchymal stem cells	746:773	human mesenchymal stem cells	746:773	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	5	28	theme	non-mineralized	1082:1096	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	29	from	hydrogels	686:694	arg1	Gradients					633:641	Gradients	633:641	Gradients of bone morphogenetic protein 2 in agarose hydrogels	633:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	3	30	theme	magnetic	446:453	arg1	alignment					461:469	magnetic field alignment	446:469	magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors,	446:561	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	5	31	theme	structural	1001:1010	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	31	theme	structural	1001:1010	arg1	features					1012:1019	biologically-relevant, emergent structural features	969:1019	biologically-relevant, emergent structural features	969:1019	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	31	theme	structural	1001:1010	arg1	interface					1126:1134	an osteochondral interface	1109:1134	an osteochondral interface rich in hypertrophic chondrocytes	1109:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	32	theme	osteochondral	795:807	arg1	constructs					816:825	robust osteochondral tissue constructs	788:825	robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage	788:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	5	33	from	rich	1136:1139	arg1	chondrocytes					1157:1168	hypertrophic chondrocytes	1144:1168	hypertrophic chondrocytes	1144:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	3	34	theme	superparamagnetic	487:503	arg1	nanoparticles					516:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	0	35	theme	nanoparticle	31:42	arg1	gradients					44:52	superparamagnetic nanoparticle gradients	13:52	superparamagnetic nanoparticle gradients for osteochondral tissue engineering	13:89	Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.					
29852377	5	36	theme	demarcating	1054:1064	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	6	37	theme	engineering	1307:1317	arg1	challenges					1319:1328	interfacial tissue engineering challenges	1288:1328	interfacial tissue engineering challenges	1288:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	1	38	theme	bioactive	131:139	arg1	signals					141:147	bioactive signals	131:147	bioactive signals	131:147	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	1	39	theme	physiological	222:234	arg1	function					236:243	physiological function	222:243	physiological function	222:243	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	0	40	theme	osteochondral	58:70	arg1	engineering					79:89	osteochondral tissue engineering	58:89	osteochondral tissue engineering	58:89	Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.					
29852377	4	41	theme	human	746:750	arg1	cells					769:773	human mesenchymal stem cells	746:773	human mesenchymal stem cells	746:773	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	6	42	theme	new	1246:1248	arg1	opportunity					1250:1260	an exciting new opportunity	1234:1260	an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges	1234:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	5	43	theme	factor	935:940	arg1	concentration					942:954	growth factor concentration	928:954	growth factor concentration	928:954	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	44	dep	biologically-relevant	969:989	arg1	emergent					992:999	emergent	992:999	emergent	992:999	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	45	theme	osteochondral	1112:1124	arg1	interface					1126:1134	an osteochondral interface	1109:1134	an osteochondral interface rich in hypertrophic chondrocytes	1109:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	46	theme	smooth	908:913	arg1	gradients					915:923	the smooth gradients	904:923	the smooth gradients in growth factor concentration	904:954	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	3	47	theme	glycosylated	474:485	arg1	nanoparticles					516:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	5	48	from	chondrocytes	1157:1168	arg1	rich					1136:1139	rich	1136:1139	rich	1136:1139	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	3	49	gly	glycosylated	474:485	arg1	nanoparticles					516:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	1	50	theme	signals	141:147	arg1	gradients					118:126	gradients	118:126	gradients of bioactive signals	118:147	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	0	51	theme	tissue	72:77	arg1	engineering					79:89	osteochondral tissue engineering	58:89	osteochondral tissue engineering	58:89	Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.					
29852377	6	52	theme	tissue	1300:1305	arg1	challenges					1319:1328	interfacial tissue engineering challenges	1288:1328	interfacial tissue engineering challenges	1288:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	5	53	theme	rich	1136:1139	arg1	interface					1126:1134	an osteochondral interface	1109:1134	an osteochondral interface rich in hypertrophic chondrocytes	1109:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	3	54	theme	systems	624:630	arg1	range					603:607	a range	601:607	a range of biomaterial systems	601:630	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	2	55	theme	constructs	367:376	arg1	success					335:341	the success	331:341	the success of bioengineered tissue constructs	331:376	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	6	56	theme	challenges	1319:1328	arg1	range					1279:1283	a range	1277:1283	a range of interfacial tissue engineering challenges	1277:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	5	57	theme	hypertrophic	1144:1155	arg1	chondrocytes					1157:1168	hypertrophic chondrocytes	1144:1168	hypertrophic chondrocytes	1144:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	58	theme	mineral	846:852	arg1	transition					854:863	a clear mineral transition	838:863	a clear mineral transition from bone to cartilage	838:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	4	59	used	used	701:704	arg2	Gradients					633:641	Gradients	633:641	Gradients of bone morphogenetic protein 2 in agarose hydrogels	633:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	2	60	theme	tissue	360:365	arg1	constructs					367:376	bioengineered tissue constructs	346:376	bioengineered tissue constructs	346:376	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	5	61	from	gradients	915:923	arg1	concentration					942:954	growth factor concentration	928:954	growth factor concentration	928:954	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	62	theme	bone	646:649	arg1	protein					665:671	bone morphogenetic protein 2	646:673	bone morphogenetic protein 2 in agarose hydrogels	646:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	4	63	from	bone	870:873	arg1	transition					854:863	a clear mineral transition	838:863	a clear mineral transition from bone to cartilage	838:886	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	5	64	theme	biologically-relevant	969:989	arg1	tissue					1098:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	a tidemark transition demarcating mineralized and non-mineralized tissue	1032:1103	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	64	theme	biologically-relevant	969:989	arg1	features					1012:1019	biologically-relevant, emergent structural features	969:1019	biologically-relevant, emergent structural features	969:1019	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	5	64	theme	biologically-relevant	969:989	arg1	interface					1126:1134	an osteochondral interface	1109:1134	an osteochondral interface rich in hypertrophic chondrocytes	1109:1168	Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes.					
29852377	4	65	theme	cells	769:773	arg1	osteogenesis					730:741	the osteogenesis	726:741	the osteogenesis of human mesenchymal stem cells	726:773	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	2	66	theme	bioengineered	346:358	arg1	constructs					367:376	bioengineered tissue constructs	346:376	bioengineered tissue constructs	346:376	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	3	67	theme	oxide	510:514	arg1	nanoparticles					516:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	glycosylated superparamagnetic iron oxide nanoparticles	474:528	In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems.					
29852377	4	68	from	protein	665:671	arg1	hydrogels					686:694	agarose hydrogels	678:694	agarose hydrogels	678:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	4	69	theme	agarose	678:684	arg1	hydrogels					686:694	agarose hydrogels	678:694	agarose hydrogels	678:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	6	70	theme	interfacial	1288:1298	arg1	challenges					1319:1328	interfacial tissue engineering challenges	1288:1328	interfacial tissue engineering challenges	1288:1328	This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.					
29852377	4	71	theme	protein	665:671	arg1	Gradients					633:641	Gradients	633:641	Gradients of bone morphogenetic protein 2 in agarose hydrogels	633:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
29852377	2	72	theme	native	273:278	arg1	features					280:287	these native features	267:287	these native features	267:287	Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs.					
29852377	1	73	from	tissue	199:204	arg1	formation					160:168	the formation	156:168	the formation of structural transitions in tissue that are key to physiological function	156:243	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	1	74	theme	structural	173:182	arg1	transitions					184:194	structural transitions	173:194	structural transitions in tissue that are key to physiological function	173:243	In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function.					
29852377	4	75	theme	morphogenetic	651:663	arg1	protein					665:671	bone morphogenetic protein 2	646:673	bone morphogenetic protein 2 in agarose hydrogels	646:694	Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage.					
30611426	4	0	theme	compositional	718:730	arg1	homogeneity					732:742	compositional homogeneity	718:742	compositional homogeneity	718:742	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	1	1	mod	modified	181:188	arg3	carbohydrates					195:207	carbohydrates	195:207	carbohydrates	195:207	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	1	1	mod	modified	181:188	arg1	majority					102:109	The majority	98:109	The majority of proteins excreted by human cells and borne at the cell surface	98:175	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	6	2	theme	biological	989:998	arg1	macromolecules					1000:1013	biological macromolecules	989:1013	biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution	989:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	5	3	theme	glycan	847:852	arg1	moieties					854:861	the glycan moieties	843:861	particular the glycan moieties themselves	832:872	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	7	4	theme	new	1224:1226	arg1	avenues					1228:1234	new avenues	1224:1234	new avenues	1224:1234	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	4	5	theme	technical	581:589	arg1	availability					620:631	a limited availability	610:631	a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition	610:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	4	5	theme	technical	581:589	arg1	barriers					591:598	technical barriers	581:598	technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition	581:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	8	6	theme	expressing	1344:1353	arg1	proteins					1369:1376	expressing and purifying proteins	1344:1376	expressing and purifying proteins from prokaryotic expression hosts	1344:1410	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
30611426	4	7	theme	limited	690:696	arg1	methods					698:704	limited methods	690:704	limited methods	690:704	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	3	8	attach	attached	333:340	arg1	proteins					345:352	proteins	345:352	proteins	345:352	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	3	8	attach	attached	333:340	arg2	carbohydrates					308:320	The carbohydrates	304:320	The carbohydrates covalently attached to proteins during maturation in the cell	304:382	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	5	9	gly	glycoproteins	811:823	arg1	glycoproteins					811:823	glycoproteins	811:823	glycoproteins and in particular the glycan moieties themselves	811:872	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	2	10	theme	fundamental	268:278	arg1	roles					280:284	fundamental roles	268:284	fundamental roles in many diseases	268:301	Glycoproteins mediate a wide range of processes and adopt fundamental roles in many diseases.					
30611426	5	11	theme	conformational	905:918	arg1	heterogeneity					920:932	conformational heterogeneity	905:932	conformational heterogeneity	905:932	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	6	12	theme	powerful	966:973	arg1	tool					975:978	a powerful tool	964:978	a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution	964:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	6	12	theme	powerful	966:973	arg1	spectroscopy					948:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	8	13	theme	expression	1395:1404	arg1	hosts					1406:1410	prokaryotic expression hosts	1383:1410	prokaryotic expression hosts	1383:1410	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
30611426	7	14	gly	glycoproteins	1189:1201	arg1	glycoproteins					1189:1201	glycoproteins	1189:1201	glycoproteins	1189:1201	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	3	15	theme	integral	434:441	arg1	components					461:470	integral and indispensable components	434:470	components	461:470	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	6	16	theme	molecules	1068:1076	arg1	elements					1056:1063	mobile elements	1049:1063	mobile elements of molecules with atomic-level resolution	1049:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	1	17	dep	proteins	114:121	arg1	excreted					123:130	excreted	123:130	excreted by human cells	123:145	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	1	17	dep	proteins	114:121	arg1	borne					151:155	borne	151:155	borne at the cell surface	151:175	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	8	18	theme	purifying	1359:1367	arg1	proteins					1369:1376	expressing and purifying proteins	1344:1376	expressing and purifying proteins from prokaryotic expression hosts	1344:1410	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
30611426	1	19	theme	cell	164:167	arg1	surface					169:175	the cell surface	160:175	the cell surface	160:175	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	6	20	theme	NMR	944:946	arg1	tool					975:978	a powerful tool	964:978	a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution	964:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	6	20	theme	NMR	944:946	arg1	spectroscopy					948:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	0	21	theme	Solution	20:27	arg1	Spectroscopy					33:44	Solution NMR Spectroscopy	20:44	Solution NMR Spectroscopy	20:44	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	2	22	theme	many	289:292	arg1	diseases					294:301	many diseases	289:301	many diseases	289:301	Glycoproteins mediate a wide range of processes and adopt fundamental roles in many diseases.					
30611426	4	23	theme	protein	660:666	arg1	platforms					679:687	appropriate recombinant protein expression platforms	636:687	appropriate recombinant protein expression platforms	636:687	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	5	24	theme	high	890:893	arg1	degree					895:900	a high degree	888:900	a high degree of conformational heterogeneity	888:932	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	6	25	theme	mobile	1049:1054	arg1	elements					1056:1063	mobile elements	1049:1063	mobile elements of molecules with atomic-level resolution	1049:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	5	26	dep	particular	832:841	arg1	moieties					854:861	the glycan moieties	843:861	particular the glycan moieties themselves	832:872	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	4	27	theme	recombinant	648:658	arg1	platforms					679:687	appropriate recombinant protein expression platforms	636:687	appropriate recombinant protein expression platforms	636:687	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	7	28	dep	Methods	1108:1114	arg1	incorporate					1142:1152	incorporate	1142:1152	incorporate stable isotope labels	1142:1174	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	7	28	dep	Methods	1108:1114	arg1	analyze					1181:1187	analyze	1181:1187	analyze glycoproteins	1181:1201	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	7	28	dep	Methods	1108:1114	arg1	express					1119:1125	express	1119:1125	express glycoproteins	1119:1139	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	7	29	theme	isotope	1161:1167	arg1	labels					1169:1174	stable isotope labels	1154:1174	stable isotope labels	1154:1174	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	4	30	theme	platforms	679:687	arg1	availability					620:631	a limited availability	610:631	a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition	610:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	7	31	gly	glycoproteins	1263:1275	arg1	glycoproteins					1263:1275	glycoproteins	1263:1275	glycoproteins	1263:1275	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	8	32	theme	many	1310:1313	arg1	laboratories					1315:1326	many laboratories	1310:1326	many laboratories	1310:1326	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
30611426	3	33	theme	indispensable	447:459	arg1	components					461:470	integral and indispensable components	434:470	components	461:470	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	0	34	theme	NMR	29:31	arg1	Spectroscopy					33:44	Solution NMR Spectroscopy	20:44	Solution NMR Spectroscopy	20:44	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	4	35	gly	glycoprotein	772:783	arg1	glycoprotein					772:783	glycoprotein composition	772:795	glycoprotein composition	772:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	6	36	theme	Solution	935:942	arg1	tool					975:978	a powerful tool	964:978	a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution	964:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	6	36	theme	Solution	935:942	arg1	spectroscopy					948:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy	935:959	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	4	37	theme	expression	668:677	arg1	platforms					679:687	appropriate recombinant protein expression platforms	636:687	appropriate recombinant protein expression platforms	636:687	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	0	38	theme	Human	49:53	arg1	Glycoproteins					55:67	Human Glycoproteins	49:67	Human Glycoproteins	49:67	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	4	39	theme	glycoproteins	520:532	arg1	structure					507:515	the structure	503:515	the structure of glycoproteins to high resolution	503:551	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	3	40	from	maturation	361:370	arg1	cell					379:382	the cell	375:382	the cell	375:382	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	4	41	theme	difficulties	749:760	arg1	availability					620:631	a limited availability	610:631	a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition	610:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	3	42	theme	protein	400:406	arg1	structure					408:416	protein structure	400:416	protein structure	400:416	The carbohydrates covalently attached to proteins during maturation in the cell directly impact protein structure and function as integral and indispensable components.					
30611426	7	43	theme	stable	1154:1159	arg1	labels					1169:1174	stable isotope labels	1154:1174	stable isotope labels	1154:1174	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	1	44	theme	proteins	114:121	arg1	majority					102:109	The majority	98:109	The majority of proteins excreted by human cells and borne at the cell surface	98:175	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	4	45	theme	high	537:540	arg1	resolution					542:551	high resolution	537:551	high resolution	537:551	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	2	46	theme	processes	248:256	arg1	range					239:243	a wide range	232:243	a wide range of processes	232:256	Glycoproteins mediate a wide range of processes and adopt fundamental roles in many diseases.					
30611426	8	47	theme	prokaryotic	1383:1393	arg1	hosts					1406:1410	prokaryotic expression hosts	1383:1410	prokaryotic expression hosts	1383:1410	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
30611426	4	48	theme	glycoprotein	772:783	arg1	composition					785:795	glycoprotein composition	772:795	glycoprotein composition	772:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	5	49	from	glycoproteins	811:823	arg1	particular					832:841	particular	832:841	particular	832:841	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	6	50	with	molecules	1068:1076	arg1	resolution					1096:1105	atomic-level resolution	1083:1105	atomic-level resolution	1083:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	4	51	gly	glycoproteins	520:532	arg1	glycoproteins					520:532	glycoproteins	520:532	glycoproteins	520:532	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	7	52	gly	glycoproteins	1127:1139	arg1	glycoproteins					1127:1139	glycoproteins	1127:1139	glycoproteins	1127:1139	Methods to express glycoproteins, incorporate stable isotope labels, and analyze glycoproteins have recently opened new avenues to prepare and investigate glycoproteins.					
30611426	2	53	from	roles	280:284	arg1	diseases					294:301	many diseases	289:301	many diseases	289:301	Glycoproteins mediate a wide range of processes and adopt fundamental roles in many diseases.					
30611426	4	54	theme	limited	612:618	arg1	availability					620:631	a limited availability	610:631	a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition	610:795	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	5	55	theme	heterogeneity	920:932	arg1	degree					895:900	a high degree	888:900	a high degree of conformational heterogeneity	888:932	Furthermore, glycoproteins and in particular the glycan moieties themselves often exhibit a high degree of conformational heterogeneity.					
30611426	0	56	dep	Preparation	4:14	arg1	The					0:2	The	0:2	The	0:2	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	1	57	theme	human	135:139	arg1	cells					141:145	human cells	135:145	human cells	135:145	The majority of proteins excreted by human cells and borne at the cell surface are modified with carbohydrates.					
30611426	6	58	theme	atomic-level	1083:1094	arg1	resolution					1096:1105	atomic-level resolution	1083:1105	atomic-level resolution	1083:1105	Solution NMR spectroscopy is a powerful tool to study biological macromolecules that is capable of characterizing mobile elements of molecules with atomic-level resolution.					
30611426	4	59	theme	appropriate	636:646	arg1	platforms					679:687	appropriate recombinant protein expression platforms	636:687	appropriate recombinant protein expression platforms	636:687	However, the ability to study the structure of glycoproteins to high resolution was historically limited by technical barriers including a limited availability of appropriate recombinant protein expression platforms, limited methods to generate compositional homogeneity, and difficulties analyzing glycoprotein composition.					
30611426	0	60	theme	Glycoproteins	55:67	arg1	Spectroscopy					33:44	Solution NMR Spectroscopy	20:44	Solution NMR Spectroscopy	20:44	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	0	60	theme	Glycoproteins	55:67	arg1	Preparation					4:14	Preparation	4:14	Preparation	4:14	The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.					
30611426	2	61	theme	wide	234:237	arg1	range					239:243	a wide range	232:243	a wide range of processes	232:256	Glycoproteins mediate a wide range of processes and adopt fundamental roles in many diseases.					
30611426	8	62	from	hosts	1406:1410	arg1	proteins					1369:1376	expressing and purifying proteins	1344:1376	expressing and purifying proteins from prokaryotic expression hosts	1344:1410	These methods are accessible to many laboratories with experience expressing and purifying proteins from prokaryotic expression hosts.					
29921663	12	0	theme	Immunoglobulin	2091:2104	arg1	IgG					2109:2111	IgG	2109:2111	IgG	2109:2111	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	12	0	theme	Immunoglobulin	2091:2104	arg1	G					2106:2106	Immunoglobulin G	2091:2106	Immunoglobulin G (IgG)	2091:2112	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	14	1	theme	glycosylation	2506:2518	arg1	features					2520:2527	glycosylation features	2506:2527	glycosylation features that are different between meningococcal sepsis patients and healthy controls	2506:2605	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	7	2	theme	lower	1241:1245	arg1	fucosylation					1252:1263	lower IgG1 fucosylation	1241:1263	lower IgG1 fucosylation	1241:1263	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	10	3	theme	sepsis	1812:1817	arg1	sepsis					1852:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	9	4	theme	severe	1672:1677	arg1	outcome					1679:1685	severe outcome	1672:1685	severe outcome	1672:1685	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	11	5	theme	fulminant	1985:1993	arg1	infection					1995:2003	a more fulminant infection	1978:2003	a more fulminant infection	1978:2003	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	5	6	theme	pediatric	790:798	arg1	patients					821:828	60 pediatric meningococcal sepsis patients	787:828	60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit	787:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	5	7	theme	intensive	856:864	arg1	unit					871:874	the pediatric intensive care unit	842:874	the pediatric intensive care unit	842:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	16	8	theme	outcome	2870:2876	arg1	predictor					2878:2886	a disease outcome predictor	2860:2886	a disease outcome predictor	2860:2886	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	3	9	theme	fragment	562:569	arg1	region					591:596	the fragment crystallizable (Fc) region	558:596	the fragment crystallizable (Fc) region	558:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	15	10	theme	higher	2648:2653	arg1	susceptibility					2655:2668	a higher susceptibility	2646:2668	a higher susceptibility to meningococcal sepsis	2646:2692	These features might be indicative of a higher susceptibility to meningococcal sepsis.					
29921663	16	11	theme	disease	2810:2816	arg1	outcome					2818:2824	severe disease outcome	2803:2824	severe disease outcome	2803:2824	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	12	12	theme	humoral	2141:2147	arg1	responses					2156:2164	humoral immune responses	2141:2164	humoral immune responses	2141:2164	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	6	13	theme	severe	1104:1109	arg1	outcome					1111:1117	a severe outcome	1102:1117	a severe outcome (death or the need for amputation)	1102:1152	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	13	14	theme	Fc	2347:2348	arg1	portion					2351:2357	fragment crystallizable (Fc) portion	2322:2357	its fragment crystallizable (Fc) portion	2318:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	8	15	theme	immunologic	1421:1431	arg1	challenges					1433:1442	previous immunologic challenges	1412:1442	previous immunologic challenges	1412:1442	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	16	16	theme	severe	2803:2808	arg1	outcome					2818:2824	severe disease outcome	2803:2824	severe disease outcome	2803:2824	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	4	17	theme	Fc	628:629	arg1	glycosylation					631:643	IgG Fc glycosylation	624:643	IgG Fc glycosylation	624:643	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	5	18	theme	meningococcal	800:812	arg1	patients					821:828	60 pediatric meningococcal sepsis patients	787:828	60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit	787:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	5	19	from	differences	743:753	arg1	glycosylation					765:777	IgG Fc glycosylation	758:777	IgG Fc glycosylation	758:777	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	10	20	theme	meningococcal	1798:1810	arg1	sepsis					1852:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	8	21	theme	higher	1453:1458	arg1	susceptibility					1460:1473	a higher susceptibility	1451:1473	a higher susceptibility of these children	1451:1491	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	14	22	from	development	2428:2438	arg1	children					2467:2474	children	2467:2474	children	2467:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	10	23	from	studies	1695:1701	arg1	groups					1713:1718	larger groups	1706:1718	larger groups	1706:1718	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	4	24	theme	sepsis	714:719	arg1	susceptibility					669:682	susceptibility	669:682	susceptibility	669:682	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	4	24	theme	sepsis	714:719	arg1	severity					688:695	severity	688:695	severity	688:695	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	9	25	theme	IgG1	1568:1571	arg1	glycans					1585:1591	IgG1 hybrid-type glycans	1568:1591	IgG1 hybrid-type glycans	1568:1591	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	2	26	theme	meningococcal	286:298	arg1	infection					300:308	the meningococcal infection	282:308	the meningococcal infection itself	282:315	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	13	27	theme	crystallizable	2331:2344	arg1	portion					2351:2357	fragment crystallizable (Fc) portion	2322:2357	its fragment crystallizable (Fc) portion	2318:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	10	28	theme	Future	1688:1693	arg1	studies					1695:1701	Future studies	1688:1701	Future studies in larger groups	1688:1718	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	9	29	theme	patient	1543:1549	arg1	group					1551:1555	the young patient group	1533:1555	the young patient group	1533:1555	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	0	30	theme	IgG	15:17	arg1	Glycosylation					22:34	IgG Fc Glycosylation	15:34	IgG Fc Glycosylation	15:34	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	2	31	theme	young	238:242	arg1	children					244:251	young children	238:251	young children	238:251	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	8	32	theme	children	1484:1491	arg1	susceptibility					1460:1473	a higher susceptibility	1451:1473	a higher susceptibility of these children	1451:1491	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	13	33	from	influence	2257:2265	arg1	affinity					2283:2290	the receptor affinity	2270:2290	the receptor affinity of IgG	2270:2297	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	0	34	theme	Pediatric	67:75	arg1	Sepsis					91:96	Pediatric Meningococcal Sepsis	67:96	Pediatric Meningococcal Sepsis	67:96	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	5	35	theme	healthy	895:901	arg1	controls					903:910	46 age-matched healthy controls	880:910	46 age-matched healthy controls	880:910	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	0	36	from	Differences	0:10	arg1	Glycosylation					22:34	IgG Fc Glycosylation	15:34	IgG Fc Glycosylation	15:34	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	1	37	theme	meningococcal	109:121	arg1	sepsis					123:128	Pediatric meningococcal sepsis	99:128	Pediatric meningococcal sepsis	99:128	Pediatric meningococcal sepsis often results in morbidity and/or death, especially in young children.					
29921663	0	38	theme	Sepsis	91:96	arg1	Outcome					56:62	Outcome	56:62	Outcome of Pediatric Meningococcal Sepsis	56:96	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	5	39	theme	spectrometric	973:985	arg1	detection					987:995	mass spectrometric detection	968:995	mass spectrometric detection of tryptic IgG glycopeptides	968:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	12	40	theme	essential	2123:2131	arg1	role					2133:2136	an essential role	2120:2136	an essential role	2120:2136	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	3	41	theme	adaptive	414:421	arg1	response					430:437	the adaptive immune response	410:437	the adaptive immune response against meningococcal infections	410:470	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	7	42	theme	Meningococcal	1179:1191	arg1	patients					1200:1207	Meningococcal sepsis patients	1179:1207	Meningococcal sepsis patients under the age of 4 years	1179:1232	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	5	43	theme	liquid	941:946	arg1	chromatography					948:961	liquid chromatography	941:961	liquid chromatography	941:961	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	12	44	theme	meningococcal	2222:2234	arg1	infections					2236:2245	meningococcal infections	2222:2245	meningococcal infections	2222:2245	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	3	45	theme	meningococcal	447:459	arg1	infections					461:470	meningococcal infections	447:470	meningococcal infections	447:470	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	5	46	theme	sepsis	814:819	arg1	patients					821:828	60 pediatric meningococcal sepsis patients	787:828	60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit	787:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	6	47	with	patients	1088:1095	arg1	outcome					1170:1176	a nonsevere outcome	1158:1176	a nonsevere outcome	1158:1176	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	6	47	with	patients	1088:1095	arg1	outcome					1111:1117	a severe outcome	1102:1117	a severe outcome (death or the need for amputation)	1102:1152	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	9	48	theme	hybrid-type	1573:1583	arg1	glycans					1585:1591	IgG1 hybrid-type glycans	1568:1591	IgG1 hybrid-type glycans	1568:1591	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	6	49	dep	outcome	1111:1117	arg1	death					1120:1124	death	1120:1124	death	1120:1124	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	6	49	dep	outcome	1111:1117	arg1	need					1133:1136	the need	1129:1136	the need for amputation	1129:1151	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	11	50	theme	factors	1966:1972	arg1	Identification					1943:1956	Identification	1943:1956	Identification of risk factors for a more fulminant infection	1943:2003	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	5	51	theme	Fc	762:763	arg1	glycosylation					765:777	IgG Fc glycosylation	758:777	IgG Fc glycosylation	758:777	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	13	52	theme	Of	2248:2249	arg1	influence					2257:2265	great influence	2251:2265	Of great influence on the receptor affinity of IgG	2248:2297	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	13	52	theme	Of	2248:2249	arg1	N-glycan					2306:2313	the N-glycan	2302:2313	the N-glycan on its fragment crystallizable (Fc) portion	2302:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	14	53	theme	sepsis	2570:2575	arg1	patients					2577:2584	meningococcal sepsis patients	2556:2584	meningococcal sepsis patients	2556:2584	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	7	54	theme	healthy	1308:1314	arg1	controls					1316:1323	age-matched healthy controls	1296:1323	age-matched healthy controls	1296:1323	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	9	55	theme	sialylation	1604:1614	arg1	levels					1558:1563	levels	1558:1563	levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose	1558:1628	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	10	56	theme	young	1927:1931	arg1	children					1933:1940	young children	1927:1940	young children	1927:1940	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	12	57	theme	adaptive	2189:2196	arg1	response					2205:2212	the adaptive immune response	2185:2212	the adaptive immune response against meningococcal infections	2185:2245	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	14	58	theme	sepsis	2457:2462	arg1	development					2428:2438	the fast development	2419:2438	the fast development of meningococcal sepsis in children	2419:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	2	59	theme	fulminant	328:336	arg1	course					338:343	a more fulminant course	321:343	a more fulminant course of the disease	321:358	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	10	60	theme	Fc	1747:1748	arg1	predictor					1784:1792	a reliable predictor	1773:1792	a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children	1773:1940	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	10	60	theme	Fc	1747:1748	arg1	glycosylation					1750:1762	IgG Fc glycosylation	1743:1762	IgG Fc glycosylation	1743:1762	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	5	61	theme	IgG	1008:1010	arg1	glycopeptides					1012:1024	tryptic IgG glycopeptides	1000:1024	tryptic IgG glycopeptides	1000:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	7	62	theme	IgG1	1276:1279	arg1	bisection					1281:1289	higher IgG1 bisection	1269:1289	higher IgG1 bisection	1269:1289	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	6	63	theme	glycosylation	1043:1055	arg1	profiles					1057:1064	Fc glycosylation profiles	1040:1064	Fc glycosylation profiles	1040:1064	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	8	64	theme	previous	1412:1419	arg1	challenges					1433:1442	previous immunologic challenges	1412:1442	previous immunologic challenges	1412:1442	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	10	65	theme	IgG	1743:1745	arg1	Fc					1747:1748	IgG Fc	1743:1748	IgG Fc glycosylation	1743:1762	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	10	66	theme	significant	1866:1876	arg1	worldwide					1902:1910	significant mortality and morbidity worldwide	1866:1910	significant mortality and morbidity worldwide	1866:1910	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	14	67	theme	fast	2423:2426	arg1	development					2428:2438	the fast development	2419:2438	the fast development of meningococcal sepsis in children	2419:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	2	68	theme	disease	352:358	arg1	course					338:343	a more fulminant course	321:343	a more fulminant course of the disease	321:358	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	2	68	theme	disease	352:358	arg1	infection					300:308	the meningococcal infection	282:308	the meningococcal infection itself	282:315	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	9	69	theme	illness	1651:1657	arg1	severity					1659:1666	illness severity	1651:1666	illness severity	1651:1666	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	7	70	theme	IgG1	1247:1250	arg1	fucosylation					1252:1263	lower IgG1 fucosylation	1241:1263	lower IgG1 fucosylation	1241:1263	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	13	71	from	N-glycan	2306:2313	arg1	portion					2351:2357	fragment crystallizable (Fc) portion	2322:2357	its fragment crystallizable (Fc) portion	2318:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	3	72	theme	glycan	529:534	arg1	structure					536:544	the glycan structure	525:544	the glycan structure attached to the fragment crystallizable (Fc) region	525:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	16	73	theme	illness	2782:2788	arg1	severity					2790:2797	illness severity	2782:2797	illness severity	2782:2797	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	14	74	theme	IgG	2394:2396	arg1	glycosylation					2398:2410	IgG glycosylation	2394:2410	IgG glycosylation	2394:2410	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	11	75	theme	treatment	2041:2049	arg1	strategies					2051:2060	appropriate treatment strategies	2029:2060	appropriate treatment strategies for the individual patients	2029:2088	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	1	76	theme	young	185:189	arg1	children					191:198	young children	185:198	young children	185:198	Pediatric meningococcal sepsis often results in morbidity and/or death, especially in young children.					
29921663	5	77	theme	pediatric	846:854	arg1	unit					871:874	the pediatric intensive care unit	842:874	the pediatric intensive care unit	842:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	6	78	theme	nonsevere	1160:1168	arg1	outcome					1170:1176	a nonsevere outcome	1158:1176	a nonsevere outcome	1158:1176	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	15	79	theme	meningococcal	2673:2685	arg1	sepsis					2687:2692	meningococcal sepsis	2673:2692	meningococcal sepsis	2673:2692	These features might be indicative of a higher susceptibility to meningococcal sepsis.					
29921663	5	80	theme	care	866:869	arg1	unit					871:874	the pediatric intensive care unit	842:874	the pediatric intensive care unit	842:874	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	16	81	theme	disease	2862:2868	arg1	predictor					2878:2886	a disease outcome predictor	2860:2886	a disease outcome predictor	2860:2886	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	10	82	theme	reliable	1775:1782	arg1	predictor					1784:1792	a reliable predictor	1773:1792	a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children	1773:1940	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	10	82	theme	reliable	1775:1782	arg1	glycosylation					1750:1762	IgG Fc glycosylation	1743:1762	IgG Fc glycosylation	1743:1762	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	15	83	theme	susceptibility	2655:2668	arg1	indicative					2632:2641	indicative	2632:2641	indicative	2632:2641	These features might be indicative of a higher susceptibility to meningococcal sepsis.					
29921663	8	84	theme	challenges	1433:1442	arg1	reflection					1398:1407	a reflection	1396:1407	a reflection of previous immunologic challenges	1396:1442	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	8	84	theme	challenges	1433:1442	arg1	it					1381:1382	it	1381:1382	it	1381:1382	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	10	85	theme	larger	1706:1711	arg1	groups					1713:1718	larger groups	1706:1718	larger groups	1706:1718	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	10	86	theme	Meningococcal	1838:1850	arg1	sepsis					1852:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	14	87	from	sepsis	2457:2462	arg1	children					2467:2474	children	2467:2474	children	2467:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	4	88	theme	IgG	624:626	arg1	glycosylation					631:643	IgG Fc glycosylation	624:643	IgG Fc glycosylation	624:643	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	3	89	theme	crystallizable	571:584	arg1	region					591:596	the fragment crystallizable (Fc) region	558:596	the fragment crystallizable (Fc) region	558:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	14	90	from	children	2467:2474	arg1	development					2428:2438	the fast development	2419:2438	the fast development of meningococcal sepsis in children	2419:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	14	91	theme	present	2367:2373	arg1	study					2375:2379	the present study	2363:2379	the present study	2363:2379	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	10	92	theme	mortality	1878:1886	arg1	worldwide					1902:1910	significant mortality and morbidity worldwide	1866:1910	significant mortality and morbidity worldwide	1866:1910	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	13	93	theme	fragment	2322:2329	arg1	portion					2351:2357	fragment crystallizable (Fc) portion	2322:2357	its fragment crystallizable (Fc) portion	2318:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	11	94	theme	individual	2070:2079	arg1	patients					2081:2088	the individual patients	2066:2088	the individual patients	2066:2088	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	6	95	theme	Fc	1040:1041	arg1	profiles					1057:1064	Fc glycosylation profiles	1040:1064	Fc glycosylation profiles	1040:1064	In addition, Fc glycosylation profiles were compared between patients with a severe outcome (death or the need for amputation) and a nonsevere outcome.					
29921663	5	96	theme	mass	968:971	arg1	detection					987:995	mass spectrometric detection	968:995	mass spectrometric detection of tryptic IgG glycopeptides	968:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	4	97	theme	meningococcal	700:712	arg1	sepsis					714:719	meningococcal sepsis	700:719	meningococcal sepsis	700:719	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	7	98	theme	4 years	1226:1232	arg1	age					1219:1221	the age	1215:1221	the age of 4 years	1215:1232	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	13	99	theme	IgG	2295:2297	arg1	affinity					2283:2290	the receptor affinity	2270:2290	the receptor affinity of IgG	2270:2297	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	0	100	theme	Meningococcal	77:89	arg1	Sepsis					91:96	Pediatric Meningococcal Sepsis	67:96	Pediatric Meningococcal Sepsis	67:96	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	5	101	theme	age-matched	883:893	arg1	controls					903:910	46 age-matched healthy controls	880:910	46 age-matched healthy controls	880:910	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	8	102	theme	meningococcal	1504:1516	arg1	sepsis					1518:1523	meningococcal sepsis	1504:1523	meningococcal sepsis	1504:1523	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	9	103	theme	young	1537:1541	arg1	group					1551:1555	the young patient group	1533:1555	the young patient group	1533:1555	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	4	104	dep	susceptibility	669:682	arg1	the					665:667	the	665:667	the	665:667	It was hypothesized that IgG Fc glycosylation might be related to the susceptibility and severity of meningococcal sepsis.					
29921663	8	105	theme	direct	1342:1347	arg1	effect					1349:1354	a direct effect	1340:1354	a direct effect of the disease	1340:1369	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	8	105	theme	direct	1342:1347	arg1	This					1326:1329	This	1326:1329	This	1326:1329	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	7	106	theme	sepsis	1193:1198	arg1	patients					1200:1207	Meningococcal sepsis patients	1179:1207	Meningococcal sepsis patients under the age of 4 years	1179:1232	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	14	107	theme	healthy	2590:2596	arg1	controls					2598:2605	healthy controls	2590:2605	healthy controls	2590:2605	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	3	108	theme	immune	423:428	arg1	response					430:437	the adaptive immune response	410:437	the adaptive immune response against meningococcal infections	410:470	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	3	109	attach	attached	546:553	arg2	structure					536:544	the glycan structure	525:544	the glycan structure attached to the fragment crystallizable (Fc) region	525:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	3	109	attach	attached	546:553	arg1	region					591:596	the fragment crystallizable (Fc) region	558:596	the fragment crystallizable (Fc) region	558:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	2	110	theme	reasons	226:232	arg1	understanding					205:217	Our understanding	201:217	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease	201:358	Our understanding of the reasons why young children are more susceptible to both the meningococcal infection itself and a more fulminant course of the disease is limited.					
29921663	14	111	gly	glycosylation	2398:2410	arg1	study					2375:2379	the present study	2363:2379	the present study	2363:2379	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	8	112	theme	disease	1363:1369	arg1	effect					1349:1354	a direct effect	1340:1354	a direct effect of the disease	1340:1369	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	8	112	theme	disease	1363:1369	arg1	This					1326:1329	This	1326:1329	This	1326:1329	This might be a direct effect of the disease; however, it can also be a reflection of previous immunologic challenges and/or a higher susceptibility of these children to develop meningococcal sepsis.					
29921663	0	113	theme	Fc	19:20	arg1	Glycosylation					22:34	IgG Fc Glycosylation	15:34	IgG Fc Glycosylation	15:34	Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis.					
29921663	13	114	theme	receptor	2274:2281	arg1	affinity					2283:2290	the receptor affinity	2270:2290	the receptor affinity of IgG	2270:2297	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	1	115	theme	Pediatric	99:107	arg1	sepsis					123:128	Pediatric meningococcal sepsis	99:128	Pediatric meningococcal sepsis	99:128	Pediatric meningococcal sepsis often results in morbidity and/or death, especially in young children.					
29921663	3	116	theme	Fc	587:588	arg1	region					591:596	the fragment crystallizable (Fc) region	558:596	the fragment crystallizable (Fc) region	558:596	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	3	117	theme	Immunoglobulin	372:385	arg1	G					387:387	Immunoglobulin G	372:387	Immunoglobulin G (IgG)	372:393	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	3	117	theme	Immunoglobulin	372:385	arg1	IgG					390:392	IgG	390:392	IgG	390:392	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	5	118	theme	IgG	758:760	arg1	glycosylation					765:777	IgG Fc glycosylation	758:777	IgG Fc glycosylation	758:777	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	9	119	theme	glycans	1585:1591	arg1	levels					1558:1563	levels	1558:1563	levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose	1558:1628	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	7	120	theme	age-matched	1296:1306	arg1	controls					1316:1323	age-matched healthy controls	1296:1323	age-matched healthy controls	1296:1323	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	10	121	theme	morbidity	1892:1900	arg1	worldwide					1902:1910	significant mortality and morbidity worldwide	1866:1910	significant mortality and morbidity worldwide	1866:1910	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	13	122	theme	great	2251:2255	arg1	influence					2257:2265	great influence	2251:2265	Of great influence on the receptor affinity of IgG	2248:2297	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	13	122	theme	great	2251:2255	arg1	N-glycan					2306:2313	the N-glycan	2302:2313	the N-glycan on its fragment crystallizable (Fc) portion	2302:2357	Of great influence on the receptor affinity of IgG is the N-glycan on its fragment crystallizable (Fc) portion.					
29921663	14	123	theme	meningococcal	2556:2568	arg1	patients					2577:2584	meningococcal sepsis patients	2556:2584	meningococcal sepsis patients	2556:2584	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	9	124	theme	IgG2/3	1597:1602	arg1	sialylation					1604:1614	IgG2/3 sialylation	1597:1614	IgG2/3 sialylation per galactose	1597:1628	Within the young patient group, levels of IgG1 hybrid-type glycans and IgG2/3 sialylation per galactose were associated with illness severity and severe outcome.					
29921663	5	125	gly	glycopeptides	1012:1024	arg2	glycopeptides					1012:1024	tryptic IgG glycopeptides	1000:1024	tryptic IgG glycopeptides	1000:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	3	126	theme	effector	481:488	arg1	functions					490:498	its effector functions	477:498	its effector functions	477:498	Immunoglobulin G (IgG) is involved in the adaptive immune response against meningococcal infections, and its effector functions are highly influenced by the glycan structure attached to the fragment crystallizable (Fc) region.					
29921663	5	127	theme	tryptic	1000:1006	arg1	glycopeptides					1012:1024	tryptic IgG glycopeptides	1000:1024	tryptic IgG glycopeptides	1000:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	10	128	theme	outcome.IMPORTANCE	1819:1836	arg1	sepsis					1852:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis	1798:1857	Future studies in larger groups should explore whether IgG Fc glycosylation could be a reliable predictor for meningococcal sepsis outcome.IMPORTANCE Meningococcal sepsis causes significant mortality and morbidity worldwide, especially in young children.					
29921663	7	129	theme	higher	1269:1274	arg1	bisection					1281:1289	higher IgG1 bisection	1269:1289	higher IgG1 bisection	1269:1289	Meningococcal sepsis patients under the age of 4 years showed lower IgG1 fucosylation and higher IgG1 bisection than age-matched healthy controls.					
29921663	14	130	theme	meningococcal	2443:2455	arg1	sepsis					2457:2462	meningococcal sepsis	2443:2462	meningococcal sepsis in children	2443:2474	In the present study, we analyzed IgG glycosylation during the fast development of meningococcal sepsis in children, and we were able to identify glycosylation features that are different between meningococcal sepsis patients and healthy controls.					
29921663	11	131	theme	appropriate	2029:2039	arg1	strategies					2051:2060	appropriate treatment strategies	2029:2060	appropriate treatment strategies for the individual patients	2029:2088	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	12	132	theme	immune	2149:2154	arg1	responses					2156:2164	humoral immune responses	2141:2164	humoral immune responses	2141:2164	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
29921663	5	133	theme	glycopeptides	1012:1024	arg1	detection					987:995	mass spectrometric detection	968:995	mass spectrometric detection of tryptic IgG glycopeptides	968:1024	Because of this, the differences in IgG Fc glycosylation between 60 pediatric meningococcal sepsis patients admitted to the pediatric intensive care unit and 46 age-matched healthy controls were investigated, employing liquid chromatography with mass spectrometric detection of tryptic IgG glycopeptides.					
29921663	16	134	theme	glycosylation	2717:2729	arg1	features					2731:2738	glycosylation features	2717:2738	glycosylation features	2717:2738	In addition, we found glycosylation features in the patients that were associated with illness severity and severe disease outcome, having the potential to serve as a disease outcome predictor.					
29921663	11	135	theme	risk	1961:1964	arg1	factors					1966:1972	risk factors	1961:1972	risk factors for a more fulminant infection	1961:2003	Identification of risk factors for a more fulminant infection would help to decide on appropriate treatment strategies for the individual patients.					
29921663	12	136	theme	immune	2198:2203	arg1	response					2205:2212	the adaptive immune response	2185:2212	the adaptive immune response against meningococcal infections	2185:2245	Immunoglobulin G (IgG) plays an essential role in humoral immune responses and is involved in the adaptive immune response against meningococcal infections.					
30348313	10	0	theme	nuclease	1422:1429	arg1	activity					1431:1438	the nuclease activity	1418:1438	the nuclease activity	1418:1438	At the same time, the modifications can be interesting in designing the nuclease activity or specificity through its glycosylation.					
30348313	9	1	used	occupied	1231:1238	arg2	sites					1217:1221	sites	1217:1221	sites	1217:1221	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	1	2	theme	enzyme	130:135	arg1	activity					137:144	enzyme activity	130:144	enzyme activity	130:144	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	8	3	theme	partial	1044:1050	arg1	demannosylation					1052:1066	The partial demannosylation	1040:1066	The partial demannosylation of R-TBN1	1040:1076	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	4	4	theme	microheterogeneity	559:576	arg1	degree					538:543	a certain degree	528:543	a certain degree of macro- and microheterogeneity	528:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	4	5	theme	high-mannose	489:500	arg1	N-glycosylation					507:521	high-mannose type N-glycosylation	489:521	high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity	489:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	5	6	theme	hypo-	635:639	arg1	mutants					672:678	hypo- and hyperglycosylated nuclease mutants	635:678	hypo- and hyperglycosylated nuclease mutants	635:678	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	1	7	theme	activity	137:144	arg1	analysis					115:122	A unique analysis	106:122	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1	106:204	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	7	8	theme	N94D	865:868	arg1	mutants					880:886	both N94D and N112D mutants	860:886	both N94D and N112D mutants	860:886	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	9	9	theme	N.	1300:1301	arg1	benthamiana					1303:1313	N. benthamiana	1300:1313	N. benthamiana	1300:1313	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	8	10	theme	nuclease	1097:1104	arg1	activity					1106:1113	the nuclease activity	1093:1113	the nuclease activity	1093:1113	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	2	11	theme	S1-P1	271:275	arg1	family					286:291	the S1-P1 nuclease family	267:291	the S1-P1 nuclease family	267:291	R-TBN1, the non-specific nuclease belonging to the S1-P1 nuclease family, was recombinantly produced in N. benthamiana.					
30348313	5	12	gly	deglycosylated	726:739	arg1	R-TBN1					719:724	R-TBN1	719:724	R-TBN1 deglycosylated by PNGase F	719:751	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	3	13	theme	native	344:349	arg1	structure					351:359	The native structure	340:359	The native structure	340:359	The native structure is posttranslationally modified by N-glycosylation at three sites.					
30348313	5	14	theme	hyperglycosylated	645:661	arg1	mutants					672:678	hypo- and hyperglycosylated nuclease mutants	635:678	hypo- and hyperglycosylated nuclease mutants	635:678	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	3	15	mod	modified	384:391	arg1	structure					351:359	The native structure	340:359	The native structure	340:359	The native structure is posttranslationally modified by N-glycosylation at three sites.					
30348313	3	15	mod	modified	384:391	arg3	N-glycosylation					396:410	N-glycosylation	396:410	N-glycosylation	396:410	The native structure is posttranslationally modified by N-glycosylation at three sites.					
30348313	5	16	theme	N-glycosylation	602:616	arg1	role					594:597	the role	590:597	the role of N-glycosylation in its activity	590:632	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	6	17	theme	mutant	794:799	arg1	N94D/N112D					801:810	mutant N94D/N112D	794:810	mutant N94D/N112D	794:810	Deglycosylated R-TBN1 and mutant N94D/N112D were virtually inactive.					
30348313	5	18	gly	hyperglycosylated	645:661	arg1	mutants					672:678	hypo- and hyperglycosylated nuclease mutants	635:678	hypo- and hyperglycosylated nuclease mutants	635:678	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	0	19	theme	enzyme	89:94	arg1	activity					96:103	the enzyme activity	85:103	the enzyme activity	85:103	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	10	20	theme	same	1357:1360	arg1	time					1362:1365	the same time	1353:1365	the same time	1353:1365	At the same time, the modifications can be interesting in designing the nuclease activity or specificity through its glycosylation.					
30348313	7	21	theme	N186D	954:958	arg1	mutants					983:989	the N186D, D36S, and D36S/E104 N mutants	950:989	the N186D, D36S, and D36S/E104 N mutants	950:989	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	7	22	theme	R-TBN1	849:854	arg1	wt					856:857	R-TBN1 wt	849:857	R-TBN1 wt	849:857	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	1	23	theme	structure	153:161	arg1	modification					163:174	structure modification	153:174	structure modification	153:174	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	8	24	from	shift	1135:1139	arg1	specificity					1154:1164	substrate specificity	1144:1164	substrate specificity	1144:1164	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	1	25	theme	modification	163:174	arg1	analysis					115:122	A unique analysis	106:122	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1	106:204	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	4	26	mod	modified	477:484	arg1	nuclease					465:472	this nuclease	460:472	this nuclease	460:472	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	4	26	mod	modified	477:484	arg3	N-glycosylation					507:521	high-mannose type N-glycosylation	489:521	high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity	489:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	6	27	theme	Deglycosylated	768:781	arg1	R-TBN1					783:788	Deglycosylated R-TBN1	768:788	Deglycosylated R-TBN1	768:788	Deglycosylated R-TBN1 and mutant N94D/N112D were virtually inactive.					
30348313	1	28	dep	activity	137:144	arg1	an					127:128	an	127:128	an	127:128	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	0	29	theme	nuclease	26:33	arg1	TBN1					35:38	tomato nuclease TBN1	19:38	tomato nuclease TBN1	19:38	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	4	30	theme	certain	530:536	arg1	degree					538:543	a certain degree	528:543	a certain degree of macro- and microheterogeneity	528:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	7	31	theme	activity	920:927	arg1	%					911:911	10%	909:911	10% of the activity	909:927	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	7	31	theme	activity	920:927	arg1	activity					920:927	the activity	916:927	the activity	916:927	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	7	31	theme	activity	920:927	arg1	%					903:903	about 60%	895:903	about 60%	895:903	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	4	32	with	N-glycosylation	507:521	arg1	degree					538:543	a certain degree	528:543	a certain degree of macro- and microheterogeneity	528:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	0	33	theme	tomato	19:24	arg1	TBN1					35:38	tomato nuclease TBN1	19:38	tomato nuclease TBN1	19:38	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	4	34	theme	macro-	548:553	arg1	degree					538:543	a certain degree	528:543	a certain degree of macro- and microheterogeneity	528:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	0	35	from	effect	75:80	arg1	activity					96:103	the enzyme activity	85:103	the enzyme activity	85:103	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	2	36	theme	nuclease	277:284	arg1	family					286:291	the S1-P1 nuclease family	267:291	the S1-P1 nuclease family	267:291	R-TBN1, the non-specific nuclease belonging to the S1-P1 nuclease family, was recombinantly produced in N. benthamiana.					
30348313	8	37	gly	demannosylation	1052:1066	arg1	nuclease					1097:1104	the nuclease activity	1093:1113	the nuclease activity	1093:1113	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	8	37	gly	demannosylation	1052:1066	arg1	R-TBN1					1071:1076	R-TBN1	1071:1076	R-TBN1	1071:1076	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	1	38	theme	tomato	183:188	arg1	R-TBN1					199:204	the tomato nuclease R-TBN1	179:204	the tomato nuclease R-TBN1	179:204	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	6	39	gly	Deglycosylated	768:781	arg1	R-TBN1					783:788	Deglycosylated R-TBN1	768:788	Deglycosylated R-TBN1	768:788	Deglycosylated R-TBN1 and mutant N94D/N112D were virtually inactive.					
30348313	0	40	gly	N-glycosylation	0:14	arg1	TBN1					35:38	tomato nuclease TBN1	19:38	tomato nuclease TBN1	19:38	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	7	41	dep	N186D	954:958	arg1	the					950:952	the	950:952	the	950:952	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	1	42	theme	nuclease	190:197	arg1	R-TBN1					199:204	the tomato nuclease R-TBN1	179:204	the tomato nuclease R-TBN1	179:204	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	0	43	theme	TBN1	35:38	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of tomato nuclease TBN1	0:38	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	9	44	dep	facts	1201:1205	arg1	1					1208:1208	1	1208:1208	1	1208:1208	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	8	45	theme	little	1128:1133	arg1	shift					1135:1139	a little shift	1126:1139	a little shift in substrate specificity	1126:1164	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	1	46	theme	R-TBN1	199:204	arg1	activity					137:144	enzyme activity	130:144	enzyme activity	130:144	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	0	47	theme	N.	52:53	arg1	benthamiana					55:65	N. benthamiana	52:65	N. benthamiana	52:65	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	4	48	theme	type	502:505	arg1	N-glycosylation					507:521	high-mannose type N-glycosylation	489:521	high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity	489:576	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	5	49	theme	nuclease	663:670	arg1	mutants					672:678	hypo- and hyperglycosylated nuclease mutants	635:678	hypo- and hyperglycosylated nuclease mutants	635:678	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	0	50	from	benthamiana	55:65	arg1	activity					96:103	the enzyme activity	85:103	the enzyme activity	85:103	N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity.					
30348313	2	51	theme	N.	324:325	arg1	benthamiana					327:337	N. benthamiana	324:337	N. benthamiana	324:337	R-TBN1, the non-specific nuclease belonging to the S1-P1 nuclease family, was recombinantly produced in N. benthamiana.					
30348313	8	52	theme	substrate	1144:1152	arg1	specificity					1154:1164	substrate specificity	1144:1164	substrate specificity	1144:1164	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	10	53	from	time	1362:1365	arg1	modifications					1372:1384	the modifications	1368:1384	the modifications	1368:1384	At the same time, the modifications can be interesting in designing the nuclease activity or specificity through its glycosylation.					
30348313	10	53	from	time	1362:1365	arg1	interesting					1393:1403	interesting	1393:1403	interesting	1393:1403	At the same time, the modifications can be interesting in designing the nuclease activity or specificity through its glycosylation.					
30348313	9	54	dep	occupied	1231:1238	arg1	glycosylates					1315:1326	glycosylates	1315:1326	glycosylates the foreign nuclease	1315:1347	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	7	55	theme	D36S/E104 N	971:981	arg1	mutants					983:989	the N186D, D36S, and D36S/E104 N mutants	950:989	the N186D, D36S, and D36S/E104 N mutants	950:989	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	9	56	dep	glycosylates	1315:1326	arg1	2					1293:1293	2	1293:1293	2	1293:1293	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	7	57	theme	D36S	961:964	arg1	mutants					983:989	the N186D, D36S, and D36S/E104 N mutants	950:989	the N186D, D36S, and D36S/E104 N mutants	950:989	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	1	58	theme	unique	108:113	arg1	analysis					115:122	A unique analysis	106:122	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1	106:204	A unique analysis of an enzyme activity versus structure modification of the tomato nuclease R-TBN1 is presented.					
30348313	2	59	theme	non-specific	232:243	arg1	R-TBN1					220:225	R-TBN1	220:225	R-TBN1	220:225	R-TBN1, the non-specific nuclease belonging to the S1-P1 nuclease family, was recombinantly produced in N. benthamiana.					
30348313	2	59	theme	non-specific	232:243	arg1	nuclease					245:252	the non-specific nuclease	228:252	the non-specific nuclease belonging to the S1-P1 nuclease family	228:291	R-TBN1, the non-specific nuclease belonging to the S1-P1 nuclease family, was recombinantly produced in N. benthamiana.					
30348313	7	60	theme	R-TBN1	1029:1034	arg1	wt					1036:1037	R-TBN1 wt	1029:1037	R-TBN1 wt	1029:1037	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	5	61	theme	PNGase	744:749	arg1	F					751:751	PNGase F	744:751	PNGase F	744:751	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	9	62	gly	glycosylates	1315:1326	arg1	nuclease					1340:1347	the foreign nuclease	1328:1347	the foreign nuclease	1328:1347	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	5	63	from	role	594:597	arg1	activity					625:632	its activity	621:632	its activity	621:632	To monitor the role of N-glycosylation in its activity, hypo- and hyperglycosylated nuclease mutants, R-TBN1 digested by α-mannosidase, and R-TBN1 deglycosylated by PNGase F were prepared.					
30348313	4	64	located	found	449:453	arg2	it					442:443	it	442:443	it	442:443	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	4	64	located	found	449:453	arg1	work					436:439	this work	431:439	this work	431:439	In this work, it was found that this nuclease is modified by high-mannose type N-glycosylation with a certain degree of macro- and microheterogeneity.					
30348313	9	65	theme	foreign	1332:1338	arg1	nuclease					1340:1347	the foreign nuclease	1328:1347	the foreign nuclease	1328:1347	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
30348313	8	66	theme	R-TBN1	1071:1076	arg1	demannosylation					1052:1066	The partial demannosylation	1040:1066	The partial demannosylation of R-TBN1	1040:1076	The partial demannosylation of R-TBN1 did not affect the nuclease activity; moreover, a little shift in substrate specificity was observed.					
30348313	7	67	theme	N112D	874:878	arg1	mutants					880:886	both N94D and N112D mutants	860:886	both N94D and N112D mutants	860:886	Compared to R-TBN1 wt, both N94D and N112D mutants showed about 60% and 10% of the activity, respectively, while the N186D, D36S, and D36S/E104 N mutants were equally or even more active than R-TBN1 wt.					
30348313	9	68	theme	proper	1260:1265	arg1	folding					1267:1273	the proper folding	1256:1273	the proper folding	1256:1273	The results show two facts: 1) which sites must be occupied by a glycan for the proper folding and stability and 2) how N. benthamiana glycosylates the foreign nuclease.					
31914594	5	0	theme	unanticipated	784:796	arg1	role					798:801	a previously unanticipated role	771:801	a previously unanticipated role for this lectin in the regulation of angiogenesis	771:851	Furthermore, we identify a previously unanticipated role for this lectin in the regulation of angiogenesis within AT.					
31914594	7	1	theme	glycosylation-dependent	1014:1036	arg1	binding					1038:1044	glycosylation-dependent binding	1014:1044	glycosylation-dependent binding to endothelial cells	1014:1065	Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis.					
31914594	9	2	theme	cell	1451:1454	arg1	function					1456:1463	endothelial cell function	1439:1463	endothelial cell function	1439:1463	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	6	3	theme	droplets	935:942	arg1	side					921:924	the inner side	911:924	the inner side of lipid droplets	911:942	Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions.					
31914594	9	4	from	AT	1419:1420	arg1	features					1375:1382	biochemical, topological, and functional features	1334:1382	biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans	1334:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	2	5	theme	insulin	367:373	arg1	resistance					375:384	insulin resistance	367:384	insulin resistance	367:384	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	4	6	theme	biophysical	577:587	arg1	determinants					589:600	biochemical and biophysical determinants	561:600	biochemical and biophysical determinants of Gal-12 structure	561:620	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	0	7	theme	glycans	108:114	arg1	recognition					79:89	preferential recognition	66:89	preferential recognition of 3-fucosylated glycans	66:114	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	6	8	theme	lipid	929:933	arg1	droplets					935:942	lipid droplets	929:942	lipid droplets	929:942	Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions.					
31914594	2	9	theme	capillary	307:315	arg1	network					317:323	its capillary network	303:323	its capillary network	303:323	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	4	10	theme	Gal-12	605:610	arg1	structure					612:620	Gal-12 structure	605:620	Gal-12 structure	605:620	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	0	11	theme	3-fucosylated	94:106	arg1	glycans					108:114	3-fucosylated glycans	94:114	3-fucosylated glycans	94:114	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	9	12	from	galectin	1407:1414	arg1	AT					1419:1420	AT	1419:1420	AT	1419:1420	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	3	13	theme	adipocyte	482:490	arg1	differentiation					492:506	adipocyte differentiation	482:506	adipocyte differentiation	482:506	Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis.					
31914594	10	14	theme	metabolic	1646:1654	arg1	disorders					1656:1664	metabolic disorders	1646:1664	metabolic disorders	1646:1664	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	9	15	theme	topological	1347:1357	arg1	features					1375:1382	biochemical, topological, and functional features	1334:1382	biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans	1334:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	9	16	theme	glycans	1502:1508	arg1	recognition					1473:1483	recognition	1473:1483	recognition of 3-fucosylated glycans	1473:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	0	17	theme	cell	48:51	arg1	function					53:60	endothelial cell function	36:60	endothelial cell function	36:60	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	6	18	theme	inner	915:919	arg1	side					921:924	the inner side	911:924	the inner side of lipid droplets	911:942	Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions.					
31914594	10	19	theme	glycosylation-dependent	1517:1539	arg1	programs					1541:1548	glycosylation-dependent programs	1517:1548	glycosylation-dependent programs	1517:1548	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	4	20	theme	structure	612:620	arg1	determinants					589:600	biochemical and biophysical determinants	561:600	biochemical and biophysical determinants of Gal-12 structure	561:620	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	1	21	theme	anabolic	189:196	arg1	pressure					198:205	anabolic pressure	189:205	anabolic pressure	189:205	Upon overnutrition, adipocytes activate a homeostatic program to adjust anabolic pressure.					
31914594	0	22	theme	adipose	3:9	arg1	galectin					18:25	An adipose tissue galectin	0:25	An adipose tissue galectin	0:25	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	9	23	theme	biochemical	1334:1344	arg1	features					1375:1382	biochemical, topological, and functional features	1334:1382	biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans	1334:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	0	24	gly	3-fucosylated	94:106	arg1	glycans					108:114	3-fucosylated glycans	94:114	3-fucosylated glycans	94:114	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	10	25	theme	endothelial	1591:1601	arg1	biology					1608:1614	endothelial cell biology	1591:1614	endothelial cell biology	1591:1614	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	4	26	theme	unique	693:698	arg1	feature					700:706	a unique feature	691:706	a unique feature among members of the galectin family	691:743	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	4	26	theme	unique	693:698	arg1	recognition					650:660	its preferential recognition	633:660	its preferential recognition of 3-fucosylated structures	633:688	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	8	27	theme	wild-type	1230:1238	arg1	mice					1240:1243	wild-type mice	1230:1243	wild-type mice	1230:1243	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	9	28	theme	functional	1364:1373	arg1	features					1375:1382	biochemical, topological, and functional features	1334:1382	biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans	1334:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	4	29	gly	3-fucosylated	665:677	arg1	structures					679:688	3-fucosylated structures	665:688	3-fucosylated structures	665:688	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	8	30	theme	AT	1283:1284	arg1	angiogenesis					1286:1297	AT angiogenesis	1283:1297	AT angiogenesis	1283:1297	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	8	31	theme	in	1129:1130	arg1	vasculature					1140:1150	in vivo AT vasculature	1129:1150	in vivo AT vasculature	1129:1150	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	3	32	theme	key	470:472	arg1	role					474:477	a key role	468:477	a key role	468:477	Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis.					
31914594	0	33	theme	tissue	11:16	arg1	galectin					18:25	An adipose tissue galectin	0:25	An adipose tissue galectin	0:25	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	6	34	theme	hypoxic	986:992	arg1	conditions					994:1003	hypoxic conditions	986:1003	hypoxic conditions	986:1003	Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions.					
31914594	10	35	theme	AT	1562:1563	arg1	homeostasis					1565:1575	AT homeostasis	1562:1575	AT homeostasis	1562:1575	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	9	36	gly	3-fucosylated	1488:1500	arg1	glycans					1502:1508	3-fucosylated glycans	1488:1508	3-fucosylated glycans	1488:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	2	37	theme	network	317:323	arg1	enlargement					288:298	concomitant enlargement	276:298	concomitant enlargement of its capillary network	276:323	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	4	38	theme	preferential	637:648	arg1	feature					700:706	a unique feature	691:706	a unique feature among members of the galectin family	691:743	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	4	38	theme	preferential	637:648	arg1	recognition					650:660	its preferential recognition	633:660	its preferential recognition of 3-fucosylated structures	633:688	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	0	39	theme	endothelial	36:46	arg1	function					53:60	endothelial cell function	36:60	endothelial cell function	36:60	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	4	40	theme	biochemical	561:571	arg1	determinants					589:600	biochemical and biophysical determinants	561:600	biochemical and biophysical determinants of Gal-12 structure	561:620	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	8	41	theme	AT	1137:1138	arg1	vasculature					1140:1150	in vivo AT vasculature	1129:1150	in vivo AT vasculature	1129:1150	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	4	42	theme	3-fucosylated	665:677	arg1	structures					679:688	3-fucosylated structures	665:688	3-fucosylated structures	665:688	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	9	43	theme	hypoxia-regulated	1389:1405	arg1	galectin					1407:1414	a hypoxia-regulated galectin	1387:1414	a hypoxia-regulated galectin	1387:1414	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	6	44	theme	preferential	878:889	arg1	localization					891:902	preferential localization	878:902	preferential localization within the inner side of lipid droplets	878:942	Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions.					
31914594	9	45	from	features	1375:1382	arg1	AT					1419:1420	AT	1419:1420	AT	1419:1420	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	8	46	dep	in	1129:1130	arg1	vivo					1132:1135	vivo	1132:1135	vivo	1132:1135	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	9	47	theme	galectin	1407:1414	arg1	features					1375:1382	biochemical, topological, and functional features	1334:1382	biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans	1334:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	10	48	theme	cell	1603:1606	arg1	biology					1608:1614	endothelial cell biology	1591:1614	endothelial cell biology	1591:1614	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	8	49	from	networks	1176:1183	arg1	Gal-12-deficient					1188:1203	Gal-12-deficient	1188:1203	Gal-12-deficient (Lgals12-/-)	1188:1216	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	8	49	from	networks	1176:1183	arg1	Lgals12-/-					1206:1215	Lgals12-/-	1206:1215	Lgals12-/-	1206:1215	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	2	50	theme	tissue	249:254	arg1	expansion					261:269	adipose tissue (AT) expansion	241:269	adipose tissue (AT) expansion	241:269	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	8	51	from	role	1259:1262	arg1	angiogenesis					1286:1297	AT angiogenesis	1283:1297	AT angiogenesis	1283:1297	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	4	52	theme	galectin	729:736	arg1	family					738:743	the galectin family	725:743	the galectin family	725:743	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	3	53	theme	endogenous	412:421	arg1	lectin					423:428	an endogenous lectin	409:428	an endogenous lectin preferentially expressed in AT	409:459	Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis.					
31914594	3	53	theme	endogenous	412:421	arg1	Galectin-12					387:397	Galectin-12	387:397	Galectin-12 (Gal-12)	387:406	Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis.					
31914594	8	54	theme	vasculature	1140:1150	arg1	analysis					1117:1124	analysis	1117:1124	analysis of in vivo AT vasculature	1117:1150	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	2	55	theme	adipose	241:247	arg1	AT					257:258	AT	257:258	AT	257:258	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	2	55	theme	adipose	241:247	arg1	tissue					249:254	adipose tissue	241:254	adipose tissue (AT) expansion	241:269	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	7	56	theme	endothelial	1049:1059	arg1	cells					1061:1065	endothelial cells	1049:1065	endothelial cells	1049:1065	Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis.					
31914594	10	57	from	implications	1630:1641	arg1	inflammation					1670:1681	inflammation	1670:1681	inflammation	1670:1681	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	10	57	from	implications	1630:1641	arg1	disorders					1656:1664	metabolic disorders	1646:1664	metabolic disorders	1646:1664	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	8	58	theme	vascular	1167:1174	arg1	networks					1176:1183	reduced vascular networks	1159:1183	reduced vascular networks in Gal-12-deficient (Lgals12-/-)	1159:1216	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	5	59	from	role	798:801	arg1	regulation					826:835	the regulation	822:835	the regulation of angiogenesis	822:851	Furthermore, we identify a previously unanticipated role for this lectin in the regulation of angiogenesis within AT.					
31914594	2	60	theme	concomitant	276:286	arg1	enlargement					288:298	concomitant enlargement	276:298	concomitant enlargement of its capillary network	276:323	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	8	61	theme	reduced	1159:1165	arg1	networks					1176:1183	reduced vascular networks	1159:1183	reduced vascular networks in Gal-12-deficient (Lgals12-/-)	1159:1216	Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis.					
31914594	9	62	theme	3-fucosylated	1488:1500	arg1	glycans					1502:1508	3-fucosylated glycans	1488:1508	3-fucosylated glycans	1488:1508	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	7	63	theme	in	1084:1085	arg1	angiogenesis					1093:1104	in vitro angiogenesis	1084:1104	in vitro angiogenesis	1084:1104	Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis.					
31914594	2	64	theme	inflammatory	211:222	arg1	response					224:231	An inflammatory response	208:231	An inflammatory response	208:231	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	2	65	theme	energy	338:343	arg1	storage					345:351	energy storage	338:351	energy storage	338:351	An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance.					
31914594	4	66	theme	family	738:743	arg1	members					714:720	members	714:720	members of the galectin family	714:743	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	0	67	theme	preferential	66:77	arg1	recognition					79:89	preferential recognition	66:89	preferential recognition of 3-fucosylated glycans	66:114	An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.					
31914594	1	68	theme	homeostatic	159:169	arg1	program					171:177	a homeostatic program	157:177	a homeostatic program to adjust anabolic pressure	157:205	Upon overnutrition, adipocytes activate a homeostatic program to adjust anabolic pressure.					
31914594	4	69	theme	structures	679:688	arg1	feature					700:706	a unique feature	691:706	a unique feature among members of the galectin family	691:743	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	4	69	theme	structures	679:688	arg1	recognition					650:660	its preferential recognition	633:660	its preferential recognition of 3-fucosylated structures	633:688	Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family.					
31914594	10	70	theme	critical	1621:1628	arg1	implications					1630:1641	critical implications	1621:1641	critical implications in metabolic disorders and inflammation	1621:1681	Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.					
31914594	5	71	theme	angiogenesis	840:851	arg1	regulation					826:835	the regulation	822:835	the regulation of angiogenesis	822:851	Furthermore, we identify a previously unanticipated role for this lectin in the regulation of angiogenesis within AT.					
31914594	3	72	theme	glucose	524:530	arg1	homeostasis					532:542	glucose homeostasis	524:542	glucose homeostasis	524:542	Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis.					
31914594	9	73	theme	endothelial	1439:1449	arg1	function					1456:1463	endothelial cell function	1439:1463	endothelial cell function	1439:1463	In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans.					
31914594	7	74	dep	in	1084:1085	arg1	vitro					1087:1091	vitro	1087:1091	vitro	1087:1091	Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis.					
30959056	0	0	theme	Potential	111:119	arg1	Enzyme					121:126	Potential Enzyme	111:126	Potential Enzyme for Mucopolysaccharidosis IVA Treatment	111:166	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	1	1	theme	keratan	369:375	arg1	sulfate					377:383	keratan sulfate	369:383	keratan sulfate	369:383	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	5	2	theme	MPS	1228:1230	arg1	therapy					1255:1261	MPS IVA enzyme replacement therapy	1228:1261	MPS IVA enzyme replacement therapy	1228:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	2	3	theme	MPS	529:531	arg1	patients					537:544	MPS IVA patients	529:544	MPS IVA patients	529:544	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	1	4	theme	sulfate	377:383	arg1	storage					358:364	lysosomal storage	348:364	lysosomal storage of keratan sulfate and chondroitin-6-sulfate	348:409	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	2	5	theme	IVA	533:535	arg1	patients					537:544	MPS IVA patients	529:544	MPS IVA patients	529:544	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	5	6	theme	therapeutic	1206:1216	arg1	GALNS					1218:1222	a therapeutic GALNS	1204:1222	a therapeutic GALNS for MPS IVA enzyme replacement therapy	1204:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	4	7	theme	N-glycosylation	927:941	arg1	structure					943:951	N-glycosylation structure	927:951	N-glycosylation structure	927:951	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	5	8	theme	replacement	1243:1253	arg1	therapy					1255:1261	MPS IVA enzyme replacement therapy	1228:1261	MPS IVA enzyme replacement therapy	1228:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	1	9	theme	N-acetylgalactosamine-6-sulfate	286:316	arg1	GALNS					329:333	GALNS	329:333	GALNS	329:333	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	9	theme	N-acetylgalactosamine-6-sulfate	286:316	arg1	sulfatase					318:326	N-acetylgalactosamine-6-sulfate sulfatase	286:326	N-acetylgalactosamine-6-sulfate sulfatase (GALNS)	286:334	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	10	theme	chondroitin-6-sulfate	389:409	arg1	storage					358:364	lysosomal storage	348:364	lysosomal storage of keratan sulfate and chondroitin-6-sulfate	348:409	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	11	dep	IVA	191:193	arg1	syndrome					217:224	Morquio A syndrome	207:224	Morquio A syndrome	207:224	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	11	dep	IVA	191:193	arg1	IVA					200:202	MPS IVA	196:202	MPS IVA	196:202	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	4	12	theme	anti-prGALNS	1052:1063	arg1	antibodies					1065:1074	anti-prGALNS antibodies	1052:1074	anti-prGALNS antibodies	1052:1074	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	1	13	theme	sulfatase	318:326	arg1	deficiency					272:281	the deficiency	268:281	the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS)	268:334	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	0	14	theme	IVA	154:156	arg1	Treatment					158:166	Mucopolysaccharidosis IVA Treatment	132:166	Mucopolysaccharidosis IVA Treatment	132:166	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	3	15	theme	similar	765:771	arg1	processing					792:801	similar post-translational processing	765:801	similar post-translational processing	765:801	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	4	16	theme	prGALNS	883:889	arg1	potential					870:878	the therapeutic potential	854:878	the therapeutic potential of prGALNS	854:889	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	1	17	theme	Morquio	207:213	arg1	syndrome					217:224	Morquio A syndrome	207:224	Morquio A syndrome	207:224	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	0	18	theme	Mucopolysaccharidosis	132:152	arg1	Treatment					158:166	Mucopolysaccharidosis IVA Treatment	132:166	Mucopolysaccharidosis IVA Treatment	132:166	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	3	19	theme	receptor-mediated	735:751	arg1	process					753:759	a potential receptor-mediated process	723:759	a potential receptor-mediated process	723:759	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	4	20	theme	antibodies	1065:1074	arg1	structure					943:951	N-glycosylation structure	927:951	N-glycosylation structure	927:951	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	20	theme	antibodies	1065:1074	arg1	generation					1038:1047	generation	1038:1047	generation	1038:1047	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	20	theme	antibodies	1065:1074	arg1	uptake					968:973	in vitro cell uptake	954:973	in vitro cell uptake	954:973	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	20	theme	antibodies	1065:1074	arg1	biodistribution					1018:1032	in vivo biodistribution	1010:1032	in vivo biodistribution	1010:1032	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	20	theme	antibodies	1065:1074	arg1	sulfate					987:993	keratan sulfate	979:993	keratan sulfate	979:993	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	5	21	theme	P. pastoris-based	1159:1175	arg1	platform					1177:1184	a P. pastoris-based platform	1157:1184	a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy	1157:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	1	22	theme	A	215:215	arg1	syndrome					217:224	Morquio A syndrome	207:224	Morquio A syndrome	207:224	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	4	23	dep	structure	943:951	arg1	the					923:925	the	923:925	the	923:925	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	3	24	theme	active	599:604	arg1	enzyme					612:617	an active GALNS enzyme	596:617	an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme	596:817	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	5	25	theme	IVA	1232:1234	arg1	therapy					1255:1261	MPS IVA enzyme replacement therapy	1228:1261	MPS IVA enzyme replacement therapy	1228:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	2	26	theme	replacement	430:440	arg1	therapy					442:448	enzyme replacement therapy	423:448	enzyme replacement therapy using an enzyme produced in CHO cells	423:486	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	5	27	theme	enzyme	1236:1241	arg1	therapy					1255:1261	MPS IVA enzyme replacement therapy	1228:1261	MPS IVA enzyme replacement therapy	1228:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	3	28	theme	GALNS	606:610	arg1	enzyme					612:617	an active GALNS enzyme	596:617	an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme	596:817	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	2	29	theme	enzyme	423:428	arg1	therapy					442:448	enzyme replacement therapy	423:448	enzyme replacement therapy using an enzyme produced in CHO cells	423:486	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	0	30	theme	Recombinant	26:36	arg1	Sulfatase					70:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	3	31	theme	human	806:810	arg1	enzyme					812:817	human enzyme	806:817	human enzyme	806:817	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	0	32	theme	Human	20:24	arg1	Sulfatase					70:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	3	33	theme	post-translational	773:790	arg1	processing					792:801	similar post-translational processing	765:801	similar post-translational processing	765:801	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	4	34	theme	uptake	968:973	arg1	characterization					903:918	the characterization	899:918	the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies	899:1074	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	35	dep	uptake	968:973	arg1	reduction					995:1003	reduction	995:1003	reduction	995:1003	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	0	36	theme	Sulfatase	70:78	arg1	Characterization					0:15	Characterization	0:15	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	0:78	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	1	37	theme	lysosomal	232:240	arg1	disease					250:256	a lysosomal storage disease	230:256	a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS)	230:334	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	37	theme	lysosomal	232:240	arg1	IVA					191:193	Mucopolysaccharidosis IVA	169:193	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome)	169:225	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	0	38	theme	N-Acetylgalactosamine-6-Sulfate	38:68	arg1	Sulfatase					70:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase	20:78	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	5	39	theme	GALNS	1218:1222	arg1	production					1190:1199	production	1190:1199	production of a therapeutic GALNS for MPS IVA enzyme replacement therapy	1190:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	4	40	theme	keratan	979:985	arg1	sulfate					987:993	keratan sulfate	979:993	keratan sulfate	979:993	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	1	41	theme	storage	242:248	arg1	disease					250:256	a lysosomal storage disease	230:256	a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS)	230:334	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	41	theme	storage	242:248	arg1	IVA					191:193	Mucopolysaccharidosis IVA	169:193	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome)	169:225	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	2	42	theme	treatment	508:516	arg1	option					518:523	the main treatment option	499:523	the main treatment option for MPS IVA patients	499:544	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	4	43	theme	biodistribution	1018:1032	arg1	characterization					903:918	the characterization	899:918	the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies	899:1074	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	3	44	theme	enzyme	612:617	arg1	production					582:591	the production	578:591	the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme	578:817	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	3	44	theme	enzyme	612:617	arg1	alternative					553:563	an alternative	550:563	an alternative	550:563	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	5	45	theme	platform	1177:1184	arg1	development					1142:1152	the development	1138:1152	the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy	1138:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	2	46	theme	main	503:506	arg1	option					518:523	the main treatment option	499:523	the main treatment option for MPS IVA patients	499:544	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	1	47	theme	lysosomal	348:356	arg1	storage					358:364	lysosomal storage	348:364	lysosomal storage of keratan sulfate and chondroitin-6-sulfate	348:409	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	4	48	theme	structure	943:951	arg1	characterization					903:918	the characterization	899:918	the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies	899:1074	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	49	theme	therapeutic	858:868	arg1	potential					870:878	the therapeutic potential	854:878	the therapeutic potential of prGALNS	854:889	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	4	50	theme	in vivo	1010:1016	arg1	biodistribution					1018:1032	in vivo biodistribution	1010:1032	in vivo biodistribution	1010:1032	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	3	51	theme	Pichia	641:646	arg1	prGALNS					658:664	prGALNS	658:664	prGALNS	658:664	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	3	51	theme	Pichia	641:646	arg1	pastoris					648:655	yeast Pichia pastoris	635:655	the yeast Pichia pastoris (prGALNS)	631:665	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	4	52	theme	cell	963:966	arg1	uptake					968:973	in vitro cell uptake	954:973	in vitro cell uptake	954:973	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	3	53	theme	cultured	700:707	arg1	cells					709:713	cultured cells	700:713	cultured cells	700:713	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	5	54	from	step	1130:1133	arg1	development					1142:1152	the development	1138:1152	the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy	1138:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	1	55	theme	Mucopolysaccharidosis	169:189	arg1	disease					250:256	a lysosomal storage disease	230:256	a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS)	230:334	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	1	55	theme	Mucopolysaccharidosis	169:189	arg1	IVA					191:193	Mucopolysaccharidosis IVA	169:193	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome)	169:225	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
30959056	4	56	theme	in vitro	954:961	arg1	uptake					968:973	in vitro cell uptake	954:973	in vitro cell uptake	954:973	In this study, we further studied the therapeutic potential of prGALNS through the characterization of the N-glycosylation structure, in vitro cell uptake and keratan sulfate reduction, and in vivo biodistribution and generation of anti-prGALNS antibodies.					
30959056	5	57	theme	important	1120:1128	arg1	step					1130:1133	an important step	1117:1133	an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy	1117:1261	Taken together, these results represent an important step in the development of a P. pastoris-based platform for production of a therapeutic GALNS for MPS IVA enzyme replacement therapy.					
30959056	3	58	theme	yeast	635:639	arg1	prGALNS					658:664	prGALNS	658:664	prGALNS	658:664	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	3	58	theme	yeast	635:639	arg1	pastoris					648:655	yeast Pichia pastoris	635:655	the yeast Pichia pastoris (prGALNS)	631:665	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	0	59	theme	Pichia	92:97	arg1	pastoris					99:106	Pichia pastoris	92:106	Pichia pastoris	92:106	Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment.					
30959056	2	60	theme	CHO	478:480	arg1	cells					482:486	CHO cells	478:486	CHO cells	478:486	Currently, enzyme replacement therapy using an enzyme produced in CHO cells represents the main treatment option for MPS IVA patients.					
30959056	3	61	theme	potential	725:733	arg1	process					753:759	a potential receptor-mediated process	723:759	a potential receptor-mediated process	723:759	As an alternative, we reported the production of an active GALNS enzyme produced in the yeast Pichia pastoris (prGALNS), which showed internalization by cultured cells through a potential receptor-mediated process and similar post-translational processing as human enzyme.					
30959056	1	62	theme	MPS	196:198	arg1	IVA					200:202	MPS IVA	196:202	MPS IVA	196:202	Mucopolysaccharidosis IVA (MPS IVA or Morquio A syndrome) is a lysosomal storage disease caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal storage of keratan sulfate and chondroitin-6-sulfate.					
31881468	0	0	from	study	14:18	arg1	absence					94:100	absence	94:100	absence	94:100	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	0	0	from	study	14:18	arg1	presence					106:113	presence	106:113	presence	106:113	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	1	1	gly	glycoprotein	176:187	arg1	MOG					190:192	MOG	190:192	MOG	190:192	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	1	1	gly	glycoprotein	176:187	arg1	glycoprotein					176:187	Myelin Oligodendrocyte glycoprotein	153:187	Myelin Oligodendrocyte glycoprotein (MOG)	153:193	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	0	2	theme	glycoprotein	74:85	arg1	states					41:46	the intermediate states	24:46	the intermediate states of myelin oligodendrocyte glycoprotein	24:85	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	4	3	theme	MOG	794:796	arg1	stability					769:777	the stability	765:777	the stability of the protein MOG	765:796	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	1	4	theme	Myelin	153:158	arg1	MOG					190:192	MOG	190:192	MOG	190:192	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	1	4	theme	Myelin	153:158	arg1	glycoprotein					176:187	Myelin Oligodendrocyte glycoprotein	153:187	Myelin Oligodendrocyte glycoprotein (MOG)	153:193	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	3	5	theme	N-glycan	584:591	arg1	presence					572:579	presence	572:579	presence	572:579	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	5	theme	N-glycan	584:591	arg1	absence					560:566	absence	560:566	absence	560:566	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	6	from	presence	572:579	arg1	folding					521:527	folding	521:527	folding	521:527	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	6	from	presence	572:579	arg1	unfolding					507:515	unfolding	507:515	unfolding	507:515	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	4	7	theme	main	695:698	arg1	results					700:706	The main results	691:706	The main results from these studies	691:725	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	1	8	theme	structural	243:252	arg1	integrity					254:262	structural integrity	243:262	structural integrity	243:262	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	6	9	theme	glycan	1153:1158	arg1	presence					1141:1148	the presence	1137:1148	the presence of glycan	1137:1158	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	6	10	theme	protein	1064:1070	arg1	folding					1072:1078	protein folding	1064:1078	protein folding	1064:1078	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	3	11	dep	stability	649:657	arg1	the					645:647	the	645:647	the	645:647	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	0	12	gly	glycoprotein	74:85	arg1	glycoprotein					74:85	myelin oligodendrocyte glycoprotein	51:85	myelin oligodendrocyte glycoprotein	51:85	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	1	13	theme	Oligodendrocyte	160:174	arg1	MOG					190:192	MOG	190:192	MOG	190:192	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	1	13	theme	Oligodendrocyte	160:174	arg1	glycoprotein					176:187	Myelin Oligodendrocyte glycoprotein	153:187	Myelin Oligodendrocyte glycoprotein (MOG)	153:193	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	3	14	from	role	620:623	arg1	stability					649:657	stability	649:657	stability	649:657	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	14	from	role	620:623	arg1	flexibility					663:673	flexibility	663:673	flexibility	663:673	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	15	theme	simulation	485:494	arg1	studies					496:502	molecular dynamics simulation studies	466:502	molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein	466:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	16	from	absence	560:566	arg1	folding					521:527	folding	521:527	folding	521:527	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	16	from	absence	560:566	arg1	unfolding					507:515	unfolding	507:515	unfolding	507:515	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	0	17	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.	0:151	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	4	18	theme	protein	786:792	arg1	MOG					794:796	the protein MOG	782:796	the protein MOG	782:796	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	3	19	theme	folding	521:527	arg1	studies					496:502	molecular dynamics simulation studies	466:502	molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein	466:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	20	theme	dynamics	476:483	arg1	studies					496:502	molecular dynamics simulation studies	466:502	molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein	466:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	21	theme	protein	682:688	arg1	stability					649:657	stability	649:657	stability	649:657	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	21	theme	protein	682:688	arg1	flexibility					663:673	flexibility	663:673	flexibility	663:673	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	5	22	theme	near-native	953:963	arg1	conformation					972:983	the near-native folded conformation	949:983	the near-native folded conformation	949:983	From the folding studies using TMD, it was observed that the glycan helps the protein to attain the near-native folded conformation.					
31881468	2	23	theme	specific	387:394	arg1	property					396:403	this specific property	382:403	this specific property of being an auto-antigen	382:428	What causes this specific property of being an auto-antigen is still not known.					
31881468	1	24	theme	myelin	267:272	arg1	sheath					274:279	myelin sheath	267:279	myelin sheath	267:279	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	0	25	theme	glycan	118:123	arg1	absence					94:100	absence	94:100	absence	94:100	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	0	25	theme	glycan	118:123	arg1	presence					106:113	presence	106:113	presence	106:113	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	3	26	theme	unfolding	507:515	arg1	studies					496:502	molecular dynamics simulation studies	466:502	molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein	466:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	6	27	theme	states	1127:1132	arg1	trace-back					1100:1109	the trace-back	1096:1109	the trace-back of intermediate states in the presence of glycan	1096:1158	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	6	28	theme	folding	1072:1078	arg1	pathway					1053:1059	the pathway	1049:1059	the pathway of protein folding	1049:1078	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	0	29	dep	absence	94:100	arg1	the					90:92	the	90:92	the	90:92	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	1	30	theme	Multiple	345:352	arg1	MS					365:366	MS	365:366	MS	365:366	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	1	30	theme	Multiple	345:352	arg1	Sclerosis					354:362	Multiple Sclerosis	345:362	Multiple Sclerosis (MS)	345:367	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	6	31	theme	TMD	1032:1034	arg1	studies					1036:1042	the direct TMD studies	1021:1042	the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan	1021:1158	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	3	32	from	MOG	544:546	arg1	presence					572:579	presence	572:579	presence	572:579	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	32	from	MOG	544:546	arg1	absence					560:566	absence	560:566	absence	560:566	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	0	33	theme	intermediate	28:39	arg1	states					41:46	the intermediate states	24:46	the intermediate states of myelin oligodendrocyte glycoprotein	24:85	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	3	34	theme	MOG	544:546	arg1	folding					521:527	folding	521:527	folding	521:527	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	34	theme	MOG	544:546	arg1	unfolding					507:515	unfolding	507:515	unfolding	507:515	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	35	dep	absence	560:566	arg1	the					556:558	the	556:558	the	556:558	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	6	36	theme	intermediate	1114:1125	arg1	states					1127:1132	intermediate states	1114:1132	intermediate states	1114:1132	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	5	37	theme	folded	965:970	arg1	conformation					972:983	the near-native folded conformation	949:983	the near-native folded conformation	949:983	From the folding studies using TMD, it was observed that the glycan helps the protein to attain the near-native folded conformation.					
31881468	1	38	theme	same	288:291	arg1	time					293:296	the same time	284:296	the same time	284:296	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	6	39	theme	direct	1025:1030	arg1	studies					1036:1042	the direct TMD studies	1021:1042	the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan	1021:1158	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	0	40	theme	computational	129:141	arg1	approach					143:150	A computational approach	127:150	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.	0:151	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	3	41	theme	protein	536:542	arg1	MOG					544:546	the protein MOG	532:546	the protein MOG in both the absence and presence of N-glycan	532:591	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	42	from	unfolding	507:515	arg1	presence					572:579	presence	572:579	presence	572:579	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	42	from	unfolding	507:515	arg1	absence					560:566	absence	560:566	absence	560:566	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	4	43	theme	MOG	837:839	arg1	unfolding					824:832	the complete unfolding	811:832	the complete unfolding of MOG in the SMD	811:850	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	5	44	theme	folding	862:868	arg1	studies					870:876	the folding studies	858:876	the folding studies using TMD	858:886	From the folding studies using TMD, it was observed that the glycan helps the protein to attain the near-native folded conformation.					
31881468	4	45	from	unfolding	824:832	arg1	SMD					848:850	the SMD	844:850	the SMD	844:850	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	0	46	dep	study	14:18	arg1	approach					143:150	A computational approach	127:150	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.	0:151	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	3	47	from	folding	521:527	arg1	presence					572:579	presence	572:579	presence	572:579	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	47	from	folding	521:527	arg1	absence					560:566	absence	560:566	absence	560:566	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	0	48	theme	oligodendrocyte	58:72	arg1	glycoprotein					74:85	myelin oligodendrocyte glycoprotein	51:85	myelin oligodendrocyte glycoprotein	51:85	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	1	49	dep	providing	233:241	arg1	acts					301:304	acts	301:304	acts as an auto-antigen which might lead to Multiple Sclerosis (MS)	301:367	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	4	50	theme	complete	815:822	arg1	unfolding					824:832	the complete unfolding	811:832	the complete unfolding of MOG in the SMD	811:850	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	6	51	from	trace-back	1100:1109	arg1	presence					1141:1148	the presence	1137:1148	the presence of glycan	1137:1158	However, it was also observed from the direct TMD studies that the pathway of protein folding was enhanced by the trace-back of intermediate states in the presence of glycan.					
31881468	1	52	theme	important	215:223	arg1	role					225:228	an important role	212:228	an important role	212:228	Myelin Oligodendrocyte glycoprotein (MOG) is found to play an important role in providing structural integrity to myelin sheath at the same time it acts as an auto-antigen which might lead to Multiple Sclerosis (MS).					
31881468	0	53	theme	myelin	51:56	arg1	glycoprotein					74:85	myelin oligodendrocyte glycoprotein	51:85	myelin oligodendrocyte glycoprotein	51:85	A comparative study for the intermediate states of myelin oligodendrocyte glycoprotein in the absence and presence of glycan - A computational approach.					
31881468	3	54	theme	glycosylation	628:640	arg1	role					620:623	the role	616:623	the role of glycosylation in the stability and flexibility of the protein	616:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	4	55	from	studies	719:725	arg1	results					700:706	The main results	691:706	The main results from these studies	691:725	The main results from these studies show that the glycosylation increases the stability of the protein MOG and inhibits the complete unfolding of MOG in the SMD.					
31881468	3	56	theme	molecular	466:474	arg1	studies					496:502	molecular dynamics simulation studies	466:502	molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein	466:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
31881468	3	57	gly	glycosylation	628:640	arg1	protein					682:688	the protein	678:688	the protein	678:688	Here we present molecular dynamics simulation studies of unfolding and folding of the protein MOG in both the absence and presence of N-glycan in order to understand the role of glycosylation in the stability and flexibility of the protein.					
30983332	0	0	theme	Mucilage	79:86	arg1	Elucidation					56:66	Elucidation	56:66	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.	0:122	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	1	1	theme	consortium	155:164	arg1	recruitment					128:138	The recruitment	124:138	The recruitment of a bacterial consortium by the host	124:176	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants.					
30983332	1	1	theme	consortium	155:164	arg1	strategy					183:190	a strategy	181:190	a strategy not limited to animals	181:213	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants.					
30983332	4	2	theme	maize	558:562	arg1	polysaccharide					564:577	the maize polysaccharide	554:577	the maize polysaccharide	554:577	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	0	3	theme	Maize	73:77	arg1	Mucilage					79:86	a Maize Mucilage	71:86	a Maize Mucilage that Harbors Diazotrophic Bacteria	71:121	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	4	4	theme	glycosidic	706:715	arg1	linkage					717:723	glycosidic linkage	706:723	glycosidic linkage	706:723	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	6	5	theme	diazotrophic	967:978	arg1	community					980:988	the diazotrophic community	963:988	the diazotrophic community	963:988	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	4	6	attach	present	522:528	arg2	we					519:520	we	519:520	we	519:520	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	4	6	attach	present	522:528	arg1	report					511:516	this report	506:516	this report	506:516	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	7	7	theme	general	1233:1239	arg1	platform					1241:1248	a general platform	1231:1248	a general platform for polysaccharide analysis in the future	1231:1290	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	7	7	theme	general	1233:1239	arg1	power					1179:1183	the power	1175:1183	the power	1175:1183	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	2	8	theme	aerial	251:256	arg1	mucilage					263:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	4	9	theme	polysaccharide	564:577	arg1	characterization					534:549	the characterization	530:549	the characterization of the maize polysaccharide	530:577	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	5	10	theme	single	763:768	arg1	polysaccharide					784:797	a single heterogeneous polysaccharide	761:797	a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	761:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	7	11	theme	polysaccharide	1254:1267	arg1	analysis					1269:1276	polysaccharide analysis	1254:1276	polysaccharide analysis in the future	1254:1290	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	2	12	theme	maize	245:249	arg1	mucilage					263:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	2	13	theme	rich	364:367	arg1	environment					369:379	the carbohydrate rich environment	347:379	the carbohydrate rich environment	347:379	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	0	14	theme	Diazotrophic	101:112	arg1	Bacteria					114:121	Diazotrophic Bacteria	101:121	Diazotrophic Bacteria	101:121	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	7	15	from	analysis	1269:1276	arg1	future					1285:1290	future	1285:1290	future	1285:1290	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	7	16	theme	methods	1203:1209	arg1	platform					1241:1248	a general platform	1231:1248	a general platform for polysaccharide analysis in the future	1231:1290	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	7	16	theme	methods	1203:1209	arg1	power					1179:1183	the power	1175:1183	the power	1175:1183	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	4	17	dep	monosaccharide	687:700	arg1	quantitation					725:736	quantitation	725:736	quantitation	725:736	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	2	18	theme	carbohydrate	351:362	arg1	environment					369:379	the carbohydrate rich environment	347:379	the carbohydrate rich environment	347:379	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	5	19	theme	galactose	848:856	arg1	backbone					858:865	a highly fucosylated and xylosylated galactose backbone	811:865	a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	811:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	5	20	contain	contains	752:759	arg2	polysaccharide					784:797	a single heterogeneous polysaccharide	761:797	a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	761:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	5	20	contain	contains	752:759	arg1	mucilage					743:750	The mucilage	739:750	The mucilage	739:750	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	6	21	theme	glycosyl	1056:1063	arg1	hydrolases					1065:1074	the glycosyl hydrolases	1052:1074	the glycosyl hydrolases associated with the microbial community	1052:1114	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	7	22	theme	analytical	1192:1201	arg1	methods					1203:1209	the analytical methods	1188:1209	the analytical methods	1188:1209	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	4	23	theme	chemical	628:635	arg1	depolymerization					637:652	chemical depolymerization	628:652	chemical depolymerization	628:652	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	0	24	theme	Structural	13:22	arg1	Elucidation					24:34	Structural Elucidation	13:34	Structural Elucidation of Polysaccharides	13:53	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	5	25	with	backbone	858:865	arg1	branches					901:908	arabinan and mannoglucuronan branches	872:908	arabinan and mannoglucuronan branches	872:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	5	26	theme	mannoglucuronan	885:899	arg1	branches					901:908	arabinan and mannoglucuronan branches	872:908	arabinan and mannoglucuronan branches	872:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	0	27	theme	Polysaccharides	39:53	arg1	Elucidation					24:34	Structural Elucidation	13:34	Structural Elucidation of Polysaccharides	13:53	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	7	28	theme	structure	1153:1161	arg1	elucidation					1121:1131	The elucidation	1117:1131	The elucidation of this complicated structure	1117:1161	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	6	29	theme	polysaccharide	923:936	arg1	structure					938:946	This unique polysaccharide structure	911:946	This unique polysaccharide structure	911:946	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	3	30	theme	synbiotic	387:395	arg1	relationship					397:408	This synbiotic relationship	382:408	This synbiotic relationship	382:408	This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown.					
30983332	5	31	theme	fucosylated	820:830	arg1	backbone					858:865	a highly fucosylated and xylosylated galactose backbone	811:865	a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	811:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	6	32	theme	unique	916:921	arg1	structure					938:946	This unique polysaccharide structure	911:946	This unique polysaccharide structure	911:946	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	6	33	theme	microbial	1096:1104	arg1	community					1106:1114	the microbial community	1092:1114	the microbial community	1092:1114	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	5	34	theme	heterogeneous	770:782	arg1	polysaccharide					784:797	a single heterogeneous polysaccharide	761:797	a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	761:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	5	35	theme	arabinan	872:879	arg1	branches					901:908	arabinan and mannoglucuronan branches	872:908	arabinan and mannoglucuronan branches	872:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	1	36	used	used	227:230	arg2	strategy					183:190	a strategy	181:190	a strategy not limited to animals	181:213	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants.					
30983332	1	36	used	used	227:230	arg2	recruitment					128:138	The recruitment	124:138	The recruitment of a bacterial consortium by the host	124:176	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants.					
30983332	2	37	theme	fixing	309:314	arg1	bacteria					316:323	nitrogen fixing bacteria	300:323	nitrogen fixing bacteria that are attracted to the carbohydrate rich environment	300:379	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	5	38	theme	xylosylated	836:846	arg1	backbone					858:865	a highly fucosylated and xylosylated galactose backbone	811:865	a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	811:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	7	39	theme	complicated	1141:1151	arg1	structure					1153:1161	this complicated structure	1136:1161	this complicated structure	1136:1161	The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.					
30983332	2	40	theme	nitrogen	300:307	arg1	bacteria					316:323	nitrogen fixing bacteria	300:323	nitrogen fixing bacteria that are attracted to the carbohydrate rich environment	300:379	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	5	41	gly	fucosylated	820:830	arg1	backbone					858:865	a highly fucosylated and xylosylated galactose backbone	811:865	a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches	811:908	The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.					
30983332	3	42	theme	complicated	452:462	arg1	structure					464:472	structure	464:472	structure	464:472	This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown.					
30983332	4	43	theme	new	592:594	arg1	strategies					607:616	new analytical strategies	592:616	new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation	592:736	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	2	44	theme	root	258:261	arg1	mucilage					263:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage	243:270	A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment.					
30983332	4	45	theme	oligosaccharide	655:669	arg1	sequencing					671:680	oligosaccharide sequencing	655:680	oligosaccharide sequencing	655:680	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
30983332	6	46	theme	containing	993:1002	arg1	monosaccharides					1004:1018	containing monosaccharides	993:1018	containing monosaccharides	993:1018	This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community.					
30983332	0	47	dep	Strategy	0:7	arg1	Elucidation					56:66	Elucidation	56:66	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.	0:122	Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.					
30983332	1	48	theme	bacterial	145:153	arg1	consortium					155:164	a bacterial consortium	143:164	a bacterial consortium	143:164	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants.					
30983332	4	49	theme	analytical	596:605	arg1	strategies					607:616	new analytical strategies	592:616	new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation	592:736	In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation.					
31846317	2	0	theme	samples	441:447	arg1	browning					429:436	browning	429:436	browning of samples	429:447	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	1	1	theme	stabilized	322:331	arg1	emulsions					333:341	their stabilized emulsions	316:341	their stabilized emulsions	316:341	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	2	2	theme	different	553:561	arg1	orders					563:568	different orders	553:568	different orders	553:568	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	1	3	from	effects	194:200	arg1	emulsions					333:341	their stabilized emulsions	316:341	their stabilized emulsions	316:341	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	3	from	effects	194:200	arg1	WPH					307:309	WPH	307:309	WPH	307:309	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	3	from	effects	194:200	arg1	hydrolysates					293:304	whey protein hydrolysates	280:304	whey protein hydrolysates (WPH)	280:310	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	6	4	theme	stability	1023:1031	arg1	Results					1004:1010	Results	1004:1010	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion	1004:1089	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	6	5	theme	emulsion	1281:1288	arg1	stability					1264:1272	the stability	1260:1272	the stability of the emulsion	1260:1288	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	6	6	theme	storage	1015:1021	arg1	stability					1023:1031	storage stability	1015:1031	storage stability	1015:1031	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	3	7	theme	far-UV	641:646	arg1	dichroism					657:665	far-UV circular dichroism	641:665	far-UV circular dichroism (CD)	641:670	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	7	theme	far-UV	641:646	arg1	CD					668:669	CD	668:669	CD	668:669	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	1	8	theme	LD	263:264	arg1	glycation					267:275	linear dextrin (LD) glycation	247:275	linear dextrin (LD) glycation	247:275	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	0	9	theme	Protein	105:111	arg1	Hydrolysates					113:124	Whey Protein Hydrolysates	100:124	Whey Protein Hydrolysates	100:124	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	3	10	theme	circular	648:655	arg1	dichroism					657:665	far-UV circular dichroism	641:665	far-UV circular dichroism (CD)	641:670	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	10	theme	circular	648:655	arg1	CD					668:669	CD	668:669	CD	668:669	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	11	contain	had	721:723	arg2	effect					739:744	a significant effect	725:744	a significant effect on secondary structures of WPH	725:775	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	11	contain	had	721:723	arg1	order					691:695	the order	687:695	the order of modification methods	687:719	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	2	12	from	SA	540:541	arg1	orders					563:568	different orders	553:568	different orders	553:568	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	0	13	theme	Whey	100:103	arg1	Hydrolysates					113:124	Whey Protein Hydrolysates	100:124	Whey Protein Hydrolysates	100:124	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	4	14	theme	surface	840:846	arg1	hydrophobicity					848:861	the surface hydrophobicity	836:861	the surface hydrophobicity	836:861	Succinylation combined with glycation effectively reduced the surface hydrophobicity and increased the molecular flexibility of WPH.					
31846317	6	15	theme	curcumin	1174:1181	arg1	bioaccessibility					1183:1198	the curcumin bioaccessibility	1170:1198	the curcumin bioaccessibility	1170:1198	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	5	16	theme	-SH	937:939	arg1	Meanwhile					911:919	Meanwhile	911:919	Meanwhile	911:919	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	5	16	theme	-SH	937:939	arg1	content					941:947	the total free -SH content	922:947	the total free -SH content	922:947	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	1	17	theme	glycation	267:275	arg1	effects					194:200	The combined effects	181:200	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions	181:341	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	2	18	theme	competitive	466:476	arg1	displacement					478:489	a competitive displacement	464:489	a competitive displacement of reactive groups	464:508	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	6	19	theme	fate	1054:1057	arg1	Results					1004:1010	Results	1004:1010	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion	1004:1089	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	1	20	theme	combined	185:192	arg1	effects					194:200	The combined effects	181:200	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions	181:341	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	3	21	theme	WPH	773:775	arg1	effect					739:744	a significant effect	725:744	a significant effect on secondary structures of WPH	725:775	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	22	dep	Fourier	599:605	arg1	transform					607:615	transform	607:615	transform infrared	607:624	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	22	dep	Fourier	599:605	arg1	ATR-FTIR					627:634	ATR-FTIR	627:634	ATR-FTIR	627:634	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	6	23	theme	gastrointestinal	1037:1052	arg1	fate					1054:1057	gastrointestinal fate	1037:1057	gastrointestinal fate	1037:1057	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	2	24	from	LD	547:548	arg1	orders					563:568	different orders	553:568	different orders	553:568	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	6	25	theme	higher	1217:1222	arg1	DG					1224:1225	higher DG	1217:1225	higher DG	1217:1225	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	6	26	theme	higher	1127:1132	arg1	DS					1134:1135	higher DS	1127:1135	higher DS	1127:1135	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	1	27	theme	succinic	205:212	arg1	SA					225:226	SA	225:226	SA	225:226	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	27	theme	succinic	205:212	arg1	anhydride					214:222	succinic anhydride	205:222	succinic anhydride (SA) succinylation	205:241	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	0	28	theme	Stabilization	19:31	arg1	Mechanism					33:41	Stabilization Mechanism	19:41	Stabilization Mechanism	19:41	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	1	29	theme	whey	280:283	arg1	WPH					307:309	WPH	307:309	WPH	307:309	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	29	theme	whey	280:283	arg1	hydrolysates					293:304	whey protein hydrolysates	280:304	whey protein hydrolysates (WPH)	280:310	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	30	theme	anhydride	214:222	arg1	succinylation					229:241	succinic anhydride (SA) succinylation	205:241	succinic anhydride (SA) succinylation	205:241	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	31	theme	protein	285:291	arg1	WPH					307:309	WPH	307:309	WPH	307:309	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	1	31	theme	protein	285:291	arg1	hydrolysates					293:304	whey protein hydrolysates	280:304	whey protein hydrolysates (WPH)	280:310	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	4	32	theme	WPH	906:908	arg1	flexibility					891:901	the molecular flexibility	877:901	the molecular flexibility of WPH	877:908	Succinylation combined with glycation effectively reduced the surface hydrophobicity and increased the molecular flexibility of WPH.					
31846317	5	33	theme	total	926:930	arg1	Meanwhile					911:919	Meanwhile	911:919	Meanwhile	911:919	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	5	33	theme	total	926:930	arg1	content					941:947	the total free -SH content	922:947	the total free -SH content	922:947	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	2	34	theme	browning	429:436	arg1	degree					389:394	degree	389:394	degree of glycation (DG)	389:412	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	34	theme	browning	429:436	arg1	Degree					359:364	Degree	359:364	Degree of succinylation (DS)	359:386	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	34	theme	browning	429:436	arg1	degree					419:424	degree	419:424	degree of browning of samples	419:447	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	5	35	theme	free	932:935	arg1	Meanwhile					911:919	Meanwhile	911:919	Meanwhile	911:919	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	5	35	theme	free	932:935	arg1	content					941:947	the total free -SH content	922:947	the total free -SH content	922:947	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	2	36	theme	groups	503:508	arg1	displacement					478:489	a competitive displacement	464:489	a competitive displacement of reactive groups	464:508	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	5	37	theme	free	976:979	arg1	content					985:991	the exposed free -SH content	964:991	the exposed free -SH content	964:991	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	2	38	theme	succinylation	369:381	arg1	degree					389:394	degree	389:394	degree of glycation (DG)	389:412	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	38	theme	succinylation	369:381	arg1	Degree					359:364	Degree	359:364	Degree of succinylation (DS)	359:386	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	38	theme	succinylation	369:381	arg1	degree					419:424	degree	419:424	degree of browning of samples	419:447	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	0	39	theme	Mechanism	33:41	arg1	Exploration					0:10	Exploration	0:10	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.	0:179	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	2	40	theme	reactive	494:501	arg1	groups					503:508	reactive groups	494:508	reactive groups	494:508	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	3	41	theme	reflection	588:597	arg1	Fourier					599:605	Attenuated total reflection Fourier	571:605	Attenuated total reflection Fourier transform infrared (ATR-FTIR)	571:635	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	42	dep	transform	607:615	arg1	infrared					617:624	infrared	617:624	transform infrared	607:624	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	0	43	theme	Bioaccessibility	56:71	arg1	Exploration					0:10	Exploration	0:10	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.	0:179	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	3	44	theme	significant	727:737	arg1	effect					739:744	a significant effect	725:744	a significant effect on secondary structures of WPH	725:775	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	45	from	effect	739:744	arg1	structures					759:768	secondary structures	749:768	secondary structures	749:768	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	3	46	theme	modification	700:711	arg1	methods					713:719	modification methods	700:719	modification methods	700:719	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	6	47	theme	modified	1109:1116	arg1	WPH					1118:1120	the modified WPH	1105:1120	the modified WPH with higher DS	1105:1135	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	6	47	theme	modified	1109:1116	arg1	effective					1146:1154	effective	1146:1154	effective	1146:1154	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	0	48	theme	Curcumin	47:54	arg1	Bioaccessibility					56:71	Curcumin Bioaccessibility	47:71	Curcumin Bioaccessibility	47:71	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	3	49	theme	methods	713:719	arg1	order					691:695	the order	687:695	the order of modification methods	687:719	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	0	50	theme	Different	163:171	arg1	Orders					173:178	Different Orders	163:178	Different Orders	163:178	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	6	51	with	WPH	1118:1120	arg1	DS					1134:1135	higher DS	1127:1135	higher DS	1127:1135	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	0	52	dep	Mechanism	33:41	arg1	the					15:17	the	15:17	the	15:17	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	5	53	theme	exposed	968:974	arg1	content					985:991	the exposed free -SH content	964:991	the exposed free -SH content	964:991	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	4	54	theme	molecular	881:889	arg1	flexibility					891:901	the molecular flexibility	877:901	the molecular flexibility of WPH	877:908	Succinylation combined with glycation effectively reduced the surface hydrophobicity and increased the molecular flexibility of WPH.					
31846317	3	55	theme	Attenuated	571:580	arg1	Fourier					599:605	Attenuated total reflection Fourier	571:605	Attenuated total reflection Fourier transform infrared (ATR-FTIR)	571:635	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	5	56	theme	-SH	981:983	arg1	content					985:991	the exposed free -SH content	964:991	the exposed free -SH content	964:991	Meanwhile, the total free -SH content decreased, and the exposed free -SH content increased.					
31846317	1	57	theme	succinylation	229:241	arg1	effects					194:200	The combined effects	181:200	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions	181:341	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	3	58	theme	total	582:586	arg1	Fourier					599:605	Attenuated total reflection Fourier	571:605	Attenuated total reflection Fourier transform infrared (ATR-FTIR)	571:635	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	6	59	theme	curcumin-loaded	1066:1080	arg1	emulsion					1082:1089	the curcumin-loaded emulsion	1062:1089	the curcumin-loaded emulsion	1062:1089	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	0	60	from	Glycation	150:158	arg1	Orders					173:178	Different Orders	163:178	Different Orders	163:178	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	0	61	theme	Emulsions	76:84	arg1	Mechanism					33:41	Stabilization Mechanism	19:41	Stabilization Mechanism	19:41	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	0	61	theme	Emulsions	76:84	arg1	Bioaccessibility					56:71	Curcumin Bioaccessibility	47:71	Curcumin Bioaccessibility	47:71	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	2	62	theme	glycation	399:407	arg1	degree					389:394	degree	389:394	degree of glycation (DG)	389:412	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	62	theme	glycation	399:407	arg1	Degree					359:364	Degree	359:364	Degree of succinylation (DS)	359:386	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	2	62	theme	glycation	399:407	arg1	degree					419:424	degree	419:424	degree of browning of samples	419:447	Degree of succinylation (DS), degree of glycation (DG), and degree of browning of samples suggested that a competitive displacement of reactive groups existed when WPH reacted with SA and LD in different orders.					
31846317	6	63	theme	emulsion	1082:1089	arg1	stability					1023:1031	storage stability	1015:1031	storage stability	1015:1031	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	6	63	theme	emulsion	1082:1089	arg1	fate					1054:1057	gastrointestinal fate	1037:1057	gastrointestinal fate	1037:1057	Results of storage stability and gastrointestinal fate of the curcumin-loaded emulsion revealed that the modified WPH with higher DS was more effective for improving the curcumin bioaccessibility, while that with higher DG was more effective for enhancing the stability of the emulsion.					
31846317	1	64	theme	linear	247:252	arg1	glycation					267:275	linear dextrin (LD) glycation	247:275	linear dextrin (LD) glycation	247:275	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
31846317	0	65	from	Succinylation	132:144	arg1	Orders					173:178	Different Orders	163:178	Different Orders	163:178	Exploration of the Stabilization Mechanism and Curcumin Bioaccessibility of Emulsions Stabilized by Whey Protein Hydrolysates after Succinylation and Glycation in Different Orders.					
31846317	3	66	theme	secondary	749:757	arg1	structures					759:768	secondary structures	749:768	secondary structures	749:768	Attenuated total reflection Fourier transform infrared (ATR-FTIR) and far-UV circular dichroism (CD) indicated that the order of modification methods had a significant effect on secondary structures of WPH.					
31846317	1	67	theme	dextrin	254:260	arg1	glycation					267:275	linear dextrin (LD) glycation	247:275	linear dextrin (LD) glycation	247:275	The combined effects of succinic anhydride (SA) succinylation and linear dextrin (LD) glycation on whey protein hydrolysates (WPH) and their stabilized emulsions were evaluated.					
30503329	2	0	dep	adhesion	338:345	arg1	elements					397:404	cell wall structural elements	376:404	cell wall structural elements	376:404	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	1	theme	important	291:299	arg1	components					311:320	important cell wall components	291:320	important cell wall components	291:320	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	1	theme	important	291:299	arg1	proteins					278:285	The cell wall proteins	264:285	The cell wall proteins	264:285	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	2	theme	wall	381:384	arg1	elements					397:404	cell wall structural elements	376:404	cell wall structural elements	376:404	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	7	3	theme	side	1105:1108	arg1	chains					1110:1115	galactofuranose side chains	1089:1115	galactofuranose side chains	1089:1115	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	1	4	theme	matrix	200:205	arg1	formation					148:156	The formation	144:156	The formation of a glucan/chitin/glycoprotein cell wall matrix	144:205	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	1	4	theme	matrix	200:205	arg1	vital					210:214	vital	210:214	vital	210:214	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	6	5	theme	present	882:888	arg1	oligosaccharides					865:880	N-linked oligosaccharides	856:880	N-linked oligosaccharides present on the glycoproteins	856:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	4	6	theme	wall	648:651	arg1	glucan					653:658	a cell wall glucan	641:658	a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	641:727	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	6	theme	wall	648:651	arg1	lichenin					631:638	lichenin	631:638	lichenin	631:638	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	13	7	theme	cell	1804:1807	arg1	wall					1809:1812	the fungal cell wall	1793:1812	the fungal cell wall	1793:1812	The pathway effectively crosslinks glycoproteins into the fungal cell wall.					
30503329	7	8	theme	α-1,6-mannose	1061:1073	arg1	backbone					1075:1082	an α-1,6-mannose backbone	1058:1082	an α-1,6-mannose backbone with galactofuranose side chains	1058:1115	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	9	9	theme	third	1217:1221	arg1	step					1223:1226	the third step	1213:1226	the third step	1213:1226	In the third step, the glucan transferases cleave the lichenin and create substrate-enzyme intermediates.					
30503329	2	10	theme	wall	306:309	arg1	components					311:320	important cell wall components	291:320	important cell wall components	291:320	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	10	theme	wall	306:309	arg1	proteins					278:285	The cell wall proteins	264:285	The cell wall proteins	264:285	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	1	11	theme	fungal	220:225	arg1	survival					227:234	fungal survival	220:234	fungal survival	220:234	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	9	12	theme	glucan	1233:1238	arg1	transferases					1240:1251	the glucan transferases	1229:1251	the glucan transferases	1229:1251	In the third step, the glucan transferases cleave the lichenin and create substrate-enzyme intermediates.					
30503329	6	13	gly	glycoproteins	897:909	arg1	glycoproteins					897:909	the glycoproteins	893:909	the glycoproteins	893:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	7	14	theme	galactomannan	1025:1037	arg1	structure					1008:1016	the structure	1004:1016	the structure	1004:1016	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	2	15	theme	cell	268:271	arg1	components					311:320	important cell wall components	291:320	important cell wall components	291:320	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	15	theme	cell	268:271	arg1	proteins					278:285	The cell wall proteins	264:285	The cell wall proteins	264:285	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	12	16	theme	cleaved	1559:1565	arg1	bond					1578:1581	the cleaved glycosidic bond	1555:1581	the cleaved glycosidic bond in lichenin	1555:1593	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	0	17	theme	cell	75:78	arg1	galactomannan					107:119	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	1	18	gly	glucan/chitin/glycoprotein	163:188	arg1	glucan/chitin/glycoprotein					163:188	a glucan/chitin/glycoprotein cell wall matrix	161:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	4	19	theme	repeating	667:675	arg1	structure					719:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	0	20	theme	glycoprotein	85:96	arg1	galactomannan					107:119	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	12	21	from	bond	1578:1581	arg1	lichenin					1586:1593	lichenin	1586:1593	lichenin	1586:1593	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	12	21	from	bond	1578:1581	arg1	energy					1543:1548	The energy	1539:1548	The energy from the cleaved glycosidic bond in lichenin	1539:1593	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	1	22	theme	cell	190:193	arg1	matrix					200:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	12	23	theme	glycosidic	1669:1678	arg1	bond					1680:1683	a new glycosidic bond	1663:1683	a new glycosidic bond between the lichenin and the processed galactomannan	1663:1736	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	3	24	gly	glycoproteins	517:529	arg1	glycoproteins					517:529	cell wall glycoproteins	507:529	cell wall glycoproteins	507:529	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	11	25	theme	glucan	1440:1445	arg1	transferases					1447:1458	the N. crassa glucan transferases	1426:1458	the N. crassa glucan transferases	1426:1458	We demonstrate that the N. crassa glucan transferases have demonstrate specificity for the processed galactomannan and for lichenin.					
30503329	0	26	theme	cell	124:127	arg1	lichenin					134:141	cell wall lichenin	124:141	cell wall lichenin	124:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	4	27	dep	in	559:560	arg1	vitro					562:566	vitro	562:566	vitro	562:566	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	28	theme	wall	738:741	arg1	glycoproteins					743:755	cell wall glycoproteins	733:755	cell wall glycoproteins	733:755	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	11	29	theme	N.	1430:1431	arg1	crassa					1433:1438	N. crassa	1430:1438	the N. crassa glucan transferases	1426:1458	We demonstrate that the N. crassa glucan transferases have demonstrate specificity for the processed galactomannan and for lichenin.					
30503329	6	30	attach	present	882:888	arg2	oligosaccharides					865:880	N-linked oligosaccharides	856:880	N-linked oligosaccharides present on the glycoproteins	856:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	6	30	attach	present	882:888	arg1	glycoproteins					897:909	the glycoproteins	893:909	the glycoproteins	893:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	0	31	theme	Neurospora	0:9	arg1	crassa					11:16	Neurospora crassa	0:16	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	12	32	used	used	1648:1651	arg2	energy					1543:1548	The energy	1539:1548	The energy from the cleaved glycosidic bond in lichenin	1539:1593	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	5	33	contain	has	833:835	arg1	pathway					778:784	the pathway	774:784	the pathway for attachment of lichenin to the glycoprotein	774:831	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
30503329	5	33	contain	has	833:835	arg2	steps					842:846	four steps	837:846	four steps	837:846	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
30503329	3	34	theme	glucan	465:470	arg1	transferases					472:483	Neurospora crassa GH72 glucan transferases	442:483	Neurospora crassa GH72 glucan transferases	442:483	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	0	35	theme	family	18:23	arg1	glucanosyltransferases					30:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	3	36	theme	crassa	453:458	arg1	transferases					472:483	Neurospora crassa GH72 glucan transferases	442:483	Neurospora crassa GH72 glucan transferases	442:483	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	0	37	link	N-linked	98:105	arg1	galactomannan					107:119	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	5	38	gly	glycoprotein	820:831	arg1	glycoprotein					820:831	the glycoprotein	816:831	the glycoprotein	816:831	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
30503329	2	39	theme	signal	348:353	arg1	transduction					355:366	signal transduction	348:366	signal transduction	348:366	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	10	40	theme	final	1323:1327	arg1	step					1329:1332	the final step	1319:1332	the final step	1319:1332	In the final step, the transferases transfer the lichenin to the processed galactomannan.					
30503329	8	41	theme	second	1125:1130	arg1	step					1132:1135	the second step	1121:1135	the second step	1121:1135	In the second step, the galactomannan is processed by members of the GH76 α-1,6-mannanases.					
30503329	3	42	theme	wall	512:515	arg1	glycoproteins					517:529	cell wall glycoproteins	507:529	cell wall glycoproteins	507:529	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	10	43	theme	processed	1381:1389	arg1	galactomannan					1391:1403	the processed galactomannan	1377:1403	the processed galactomannan	1377:1403	In the final step, the transferases transfer the lichenin to the processed galactomannan.					
30503329	2	44	theme	cell	301:304	arg1	components					311:320	important cell wall components	291:320	important cell wall components	291:320	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	44	theme	cell	301:304	arg1	proteins					278:285	The cell wall proteins	264:285	The cell wall proteins	264:285	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	7	45	theme	galactofuranose	1089:1103	arg1	chains					1110:1115	galactofuranose side chains	1089:1115	galactofuranose side chains	1089:1115	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	3	46	theme	cell	540:543	arg1	wall					545:548	the cell wall	536:548	the cell wall	536:548	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	1	47	theme	wall	195:198	arg1	matrix					200:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	4	48	with	glucan	653:658	arg1	structure					719:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	12	49	theme	substrate-enzyme	1614:1629	arg1	intermediate					1631:1642	the substrate-enzyme intermediate	1610:1642	the substrate-enzyme intermediate	1610:1642	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	13	50	gly	glycoproteins	1774:1786	arg1	glycoproteins					1774:1786	glycoproteins	1774:1786	glycoproteins	1774:1786	The pathway effectively crosslinks glycoproteins into the fungal cell wall.					
30503329	4	51	theme	cell	643:646	arg1	glucan					653:658	a cell wall glucan	641:658	a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	641:727	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	51	theme	cell	643:646	arg1	lichenin					631:638	lichenin	631:638	lichenin	631:638	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	13	52	theme	fungal	1797:1802	arg1	wall					1809:1812	the fungal cell wall	1793:1812	the fungal cell wall	1793:1812	The pathway effectively crosslinks glycoproteins into the fungal cell wall.					
30503329	5	53	attach	attachment	790:799	arg2	lichenin					804:811	lichenin	804:811	lichenin	804:811	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
30503329	5	53	attach	attachment	790:799	arg1	glycoprotein					820:831	the glycoprotein	816:831	the glycoprotein	816:831	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
30503329	8	54	theme	α-1,6-mannanases	1192:1207	arg1	members					1172:1178	members	1172:1178	members of the GH76 α-1,6-mannanases	1172:1207	In the second step, the galactomannan is processed by members of the GH76 α-1,6-mannanases.					
30503329	6	55	theme	galactomannan	945:957	arg1	addition					931:938	the addition	927:938	the addition of a galactomannan	927:957	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	4	56	theme	β-1,4-glucose-β-1,4-glucose-β-1,3-glucose	677:717	arg1	structure					719:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure	665:727	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	11	57	theme	processed	1497:1505	arg1	galactomannan					1507:1519	the processed galactomannan	1493:1519	the processed galactomannan	1493:1519	We demonstrate that the N. crassa glucan transferases have demonstrate specificity for the processed galactomannan and for lichenin.					
30503329	2	58	theme	wall	273:276	arg1	components					311:320	important cell wall components	291:320	important cell wall components	291:320	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	2	58	theme	wall	273:276	arg1	proteins					278:285	The cell wall proteins	264:285	The cell wall proteins	264:285	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	12	59	theme	processed	1714:1722	arg1	galactomannan					1724:1736	the processed galactomannan	1710:1736	the processed galactomannan	1710:1736	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	12	60	theme	glycosidic	1567:1576	arg1	bond					1578:1581	the cleaved glycosidic bond	1555:1581	the cleaved glycosidic bond in lichenin	1555:1593	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	0	61	theme	wall	80:83	arg1	galactomannan					107:119	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	0	62	theme	N-linked	98:105	arg1	galactomannan					107:119	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	7	63	theme	report	975:980	arg1	part					963:966	part	963:966	part of our report	963:980	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	1	64	theme	glucan/chitin/glycoprotein	163:188	arg1	matrix					200:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	a glucan/chitin/glycoprotein cell wall matrix	161:205	The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis.					
30503329	6	65	mod	modified	915:922	arg3	addition					931:938	the addition	927:938	the addition of a galactomannan	927:957	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	6	65	mod	modified	915:922	arg1	oligosaccharides					865:880	N-linked oligosaccharides	856:880	N-linked oligosaccharides present on the glycoproteins	856:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	9	66	theme	substrate-enzyme	1284:1299	arg1	intermediates					1301:1313	substrate-enzyme intermediates	1284:1313	substrate-enzyme intermediates	1284:1313	In the third step, the glucan transferases cleave the lichenin and create substrate-enzyme intermediates.					
30503329	11	67	theme	crassa	1433:1438	arg1	transferases					1447:1458	the N. crassa glucan transferases	1426:1458	the N. crassa glucan transferases	1426:1458	We demonstrate that the N. crassa glucan transferases have demonstrate specificity for the processed galactomannan and for lichenin.					
30503329	0	68	theme	wall	129:132	arg1	lichenin					134:141	cell wall lichenin	124:141	cell wall lichenin	124:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	0	69	gly	glycoprotein	85:96	arg1	glycoprotein					85:96	cell wall glycoprotein N-linked galactomannan	75:119	cell wall glycoprotein N-linked galactomannan to cell wall lichenin	75:141	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	12	70	theme	new	1665:1667	arg1	bond					1680:1683	a new glycosidic bond	1663:1683	a new glycosidic bond between the lichenin and the processed galactomannan	1663:1736	The energy from the cleaved glycosidic bond in lichenin is retained in the substrate-enzyme intermediate and used to create a new glycosidic bond between the lichenin and the processed galactomannan.					
30503329	4	71	gly	glycoproteins	743:755	arg1	glycoproteins					743:755	cell wall glycoproteins	733:755	cell wall glycoproteins	733:755	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	0	72	theme	crassa	11:16	arg1	glucanosyltransferases					30:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	4	73	theme	in	559:560	arg1	assay					568:572	an in vitro assay	556:572	an in vitro assay	556:572	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	74	theme	cell	733:736	arg1	glycoproteins					743:755	cell wall glycoproteins	733:755	cell wall glycoproteins	733:755	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	2	75	theme	cell	376:379	arg1	elements					397:404	cell wall structural elements	376:404	cell wall structural elements	376:404	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	0	76	theme	GH72	25:28	arg1	glucanosyltransferases					30:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases	0:51	Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.					
30503329	6	77	theme	N-linked	856:863	arg1	oligosaccharides					865:880	N-linked oligosaccharides	856:880	N-linked oligosaccharides present on the glycoproteins	856:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	7	78	with	backbone	1075:1082	arg1	chains					1110:1115	galactofuranose side chains	1089:1115	galactofuranose side chains	1089:1115	As part of our report we have characterized the structure of the galactomannan, which consists of an α-1,6-mannose backbone with galactofuranose side chains.					
30503329	3	79	theme	cell	507:510	arg1	glycoproteins					517:529	cell wall glycoproteins	507:529	cell wall glycoproteins	507:529	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	2	80	theme	structural	386:395	arg1	elements					397:404	cell wall structural elements	376:404	cell wall structural elements	376:404	The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements.					
30503329	4	81	theme	glucan	592:597	arg1	able					616:619	able	616:619	able	616:619	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	81	theme	glucan	592:597	arg1	transferases					599:610	the glucan transferases	588:610	the glucan transferases	588:610	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	82	attach	attach	624:629	arg1	glycoproteins					743:755	cell wall glycoproteins	733:755	cell wall glycoproteins	733:755	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	82	attach	attach	624:629	arg2	able					616:619	able	616:619	able	616:619	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	4	82	attach	attach	624:629	arg2	transferases					599:610	the glucan transferases	588:610	the glucan transferases	588:610	With an in vitro assay, we show that the glucan transferases are able to attach lichenin, a cell wall glucan with a repeating β-1,4-glucose-β-1,4-glucose-β-1,3-glucose structure, to cell wall glycoproteins.					
30503329	6	83	link	N-linked	856:863	arg1	oligosaccharides					865:880	N-linked oligosaccharides	856:880	N-linked oligosaccharides present on the glycoproteins	856:909	First, N-linked oligosaccharides present on the glycoproteins are modified by the addition of a galactomannan.					
30503329	3	84	theme	Neurospora	442:451	arg1	crassa					453:458	Neurospora crassa GH72	442:463	Neurospora crassa GH72 glucan transferases	442:483	In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall.					
30503329	5	85	theme	lichenin	804:811	arg1	attachment					790:799	attachment	790:799	attachment of lichenin to the glycoprotein	790:831	We propose that the pathway for attachment of lichenin to the glycoprotein has four steps.					
31526239	14	0	theme	IgG	2196:2198	arg1	alterations					2211:2221	IgG N-glycomic alterations	2196:2221	IgG N-glycomic alterations	2196:2221	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	10	1	theme	IgG	1705:1707	arg1	glycans					1709:1715	IgG glycans	1705:1715	IgG glycans alone	1705:1721	The AUC for IgG glycans alone was 0.623 with a 95% confidence interval 0.580-0.666.					
31526239	1	2	theme	environmental	247:259	arg1	factors					287:293	multiple environmental, genomic, and postgenomic factors	238:293	multiple environmental, genomic, and postgenomic factors	238:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	12	3	theme	increased	1963:1971	arg1	status					2010:2015	an increased proinflammatory and biological aging status	1960:2015	an increased proinflammatory and biological aging status	1960:2015	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	1	4	theme	genomic	262:268	arg1	factors					287:293	multiple environmental, genomic, and postgenomic factors	238:293	multiple environmental, genomic, and postgenomic factors	238:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	8	5	link	derived	1319:1325	arg1	peaks					1334:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	2	6	theme	G	383:383	arg1	patterns					400:407	immunoglobulin G (IgG) N-glycan patterns	368:407	immunoglobulin G (IgG) N-glycan patterns	368:407	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	13	7	from	T2DM	2097:2100	arg1	population					2119:2128	an Australian population	2105:2128	an Australian population	2105:2128	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	13	8	theme	Australian	2108:2117	arg1	population					2119:2128	an Australian population	2105:2128	an Australian population	2105:2128	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	14	9	theme	renal	2367:2371	arg1	failure					2373:2379	renal failure	2367:2379	renal failure	2367:2379	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	13	10	theme	proinflammatory	2155:2169	arg1	mechanisms					2171:2180	proinflammatory mechanisms	2155:2180	proinflammatory mechanisms	2155:2180	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	2	11	from	association	348:358	arg1	population					465:474	an Australian population	451:474	an Australian population	451:474	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	10	12	theme	%	1742:1742	arg1	interval					1755:1762	a 95% confidence interval 0.580-0.666	1738:1774	a 95% confidence interval 0.580-0.666	1738:1774	The AUC for IgG glycans alone was 0.623 with a 95% confidence interval 0.580-0.666.					
31526239	1	13	theme	common	185:190	arg1	mellitus					164:171	Type 2 diabetes mellitus	148:171	Type 2 diabetes mellitus (T2DM)	148:178	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	13	theme	common	185:190	arg1	trait					200:204	a common complex trait	183:204	a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors	183:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	11	14	theme	N-glycan	1864:1871	arg1	stratification					1873:1886	IgG N-glycan stratification	1860:1886	IgG N-glycan stratification	1860:1886	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	3	15	theme	modifications	571:583	arg1	one					508:510	one	508:510	one	508:510	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	3	15	theme	modifications	571:583	arg1	modifications					571:583	the most frequently observed co- and post-translational modifications	515:583	the most frequently observed co- and post-translational modifications	515:583	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	0	16	from	Mechanisms	108:117	arg1	Population					136:145	an Australian Population	122:145	an Australian Population	122:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	8	17	theme	measured	1301:1308	arg1	peaks					1334:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	14	18	theme	progression	2326:2336	arg1	diagnosis					2292:2300	T2DM diagnosis	2287:2300	T2DM diagnosis	2287:2300	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	14	18	theme	progression	2326:2336	arg1	monitoring					2307:2316	monitoring	2307:2316	monitoring of T2DM progression to cardiovascular disease or renal failure	2307:2379	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	13	19	theme	IgG	2079:2081	arg1	N-glycome					2083:2091	IgG N-glycome	2079:2091	IgG N-glycome	2079:2091	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	4	20	theme	case-control	715:726	arg1	study					728:732	a community-based case-control study	697:732	a community-based case-control study	697:732	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	8	21	theme	N-glycan	1484:1491	arg1	traits					1493:1498	IgG N-glycan traits	1480:1498	IgG N-glycan traits	1480:1498	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	7	22	theme	risk	1238:1241	arg1	factors					1243:1249	clinical risk factors	1229:1249	clinical risk factors	1229:1249	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	8	23	dep	extensive	1402:1410	arg1	clinical					1412:1419	clinical	1412:1419	clinical	1412:1419	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	2	24	theme	Australian	454:463	arg1	population					465:474	an Australian population	451:474	an Australian population	451:474	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	8	25	theme	glycan	1327:1332	arg1	peaks					1334:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	13	26	theme	novel	2048:2052	arg1	associations					2054:2065	novel associations	2048:2065	novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms	2048:2180	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	5	27	theme	liquid	885:890	arg1	method					907:912	the ultraperformance liquid chromatography method	864:912	the ultraperformance liquid chromatography method	864:912	We applied the ultraperformance liquid chromatography method to analyze the composition of IgG N-glycans.					
31526239	0	28	theme	Putative	83:90	arg1	Mechanisms					108:117	Putative Proinflammatory Mechanisms	83:117	Putative Proinflammatory Mechanisms in an Australian Population	83:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	12	29	theme	glycan	1897:1902	arg1	traits					1904:1909	Six IgG glycan traits	1889:1909	Six IgG glycan traits	1889:1909	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	6	30	theme	clinical	1076:1083	arg1	factors					1090:1096	clinical risk factors	1076:1096	clinical risk factors	1076:1096	We then conducted Spearman's correlation analyses to explore the association between glycan biomarker candidates and clinical risk factors.					
31526239	8	31	theme	risk	1541:1544	arg1	factors					1546:1552	clinical risk factors	1532:1552	T2DM clinical risk factors	1527:1552	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	4	32	dep	participants	739:750	arg1	cases					757:761	217 cases	753:761	217 cases	753:761	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	4	32	dep	participants	739:750	arg1	controls					771:778	632 controls	767:778	632 controls	767:778	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	0	33	theme	G	63:63	arg1	N-Glycome					65:73	the Immunoglobulin G N-Glycome	44:73	the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population	44:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	14	34	theme	biomarker	2262:2270	arg1	candidates					2272:2281	inflammatory biomarker candidates	2249:2281	inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure	2249:2379	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	14	34	theme	biomarker	2262:2270	arg1	prospects					2236:2244	future prospects	2229:2244	future prospects	2229:2244	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	11	35	theme	IgG	1860:1862	arg1	stratification					1873:1886	IgG N-glycan stratification	1860:1886	IgG N-glycan stratification	1860:1886	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	0	36	theme	Type	0:3	arg1	Mellitus					16:23	Type 2 Diabetes Mellitus	0:23	Type 2 Diabetes Mellitus	0:23	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	9	37	theme	IgG	1576:1578	arg1	profiles					1587:1594	the IgG glycan profiles	1572:1594	the IgG glycan profiles	1572:1594	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	0	38	theme	Diabetes	7:14	arg1	Mellitus					16:23	Type 2 Diabetes Mellitus	0:23	Type 2 Diabetes Mellitus	0:23	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	1	39	theme	postgenomic	275:285	arg1	factors					287:293	multiple environmental, genomic, and postgenomic factors	238:293	multiple environmental, genomic, and postgenomic factors	238:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	5	40	theme	IgG	944:946	arg1	N-glycans					948:956	IgG N-glycans	944:956	IgG N-glycans	944:956	We applied the ultraperformance liquid chromatography method to analyze the composition of IgG N-glycans.					
31526239	6	41	theme	glycan	1044:1049	arg1	candidates					1061:1070	glycan biomarker candidates	1044:1070	glycan biomarker candidates	1044:1070	We then conducted Spearman's correlation analyses to explore the association between glycan biomarker candidates and clinical risk factors.					
31526239	14	42	theme	future	2229:2234	arg1	candidates					2272:2281	inflammatory biomarker candidates	2249:2281	inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure	2249:2379	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	14	42	theme	future	2229:2234	arg1	prospects					2236:2244	future prospects	2229:2244	future prospects	2229:2244	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	2	43	theme	N-glycan	391:398	arg1	patterns					400:407	immunoglobulin G (IgG) N-glycan patterns	368:407	immunoglobulin G (IgG) N-glycan patterns	368:407	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	9	44	dep	0.859	1686:1690	arg1	to					1683:1684	to	1683:1684	to	1683:1684	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	13	45	theme	mechanisms	2171:2180	arg1	T2DM					2097:2100	T2DM	2097:2100	T2DM	2097:2100	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	13	45	theme	mechanisms	2171:2180	arg1	N-glycome					2083:2091	IgG N-glycome	2079:2091	IgG N-glycome	2079:2091	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	13	45	theme	mechanisms	2171:2180	arg1	role					2147:2150	the putative role	2134:2150	the putative role of proinflammatory mechanisms	2134:2180	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	8	46	dep	derived	1319:1325	arg1	four					1314:1317	four	1314:1317	four	1314:1317	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	2	47	theme	risk	435:438	arg1	factors					440:446	their clinical risk factors	420:446	their clinical risk factors	420:446	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	0	48	theme	Immunoglobulin	48:61	arg1	N-Glycome					65:73	the Immunoglobulin G N-Glycome	44:73	the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population	44:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	4	49	theme	urban	804:808	arg1	community					810:818	an urban community	801:818	an urban community in Busselton, Western Australia	801:850	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	11	50	theme	diversity	1825:1833	arg1	evidence					1813:1820	new evidence	1809:1820	new evidence of diversity	1809:1833	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	3	51	theme	genomic	661:667	arg1	factors					685:691	the genomic and postgenomic factors	657:691	the genomic and postgenomic factors	657:691	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	9	52	theme	fasting	1599:1605	arg1	glucose					1613:1619	fasting blood glucose	1599:1619	fasting blood glucose in the logistic regression model	1599:1652	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	2	53	theme	first	315:319	arg1	attempt					321:327	the first attempt	311:327	the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population	311:474	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	13	54	theme	putative	2138:2145	arg1	role					2147:2150	the putative role	2134:2150	the putative role of proinflammatory mechanisms	2134:2180	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	8	55	theme	discovery	1443:1451	arg1	rate					1453:1456	false discovery rate	1437:1456	false discovery rate	1437:1456	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	3	56	theme	proteins	496:503	arg1	N-glycosylation					477:491	N-glycosylation	477:491	N-glycosylation of proteins	477:503	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	12	57	theme	biological	1993:2002	arg1	status					2010:2015	an increased proinflammatory and biological aging status	1960:2015	an increased proinflammatory and biological aging status	1960:2015	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	9	58	theme	regression	1637:1646	arg1	model					1648:1652	the logistic regression model	1624:1652	the logistic regression model	1624:1652	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	14	59	theme	N-glycomic	2200:2209	arg1	alterations					2211:2221	IgG N-glycomic alterations	2196:2221	IgG N-glycomic alterations	2196:2221	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	11	60	theme	T2DM	1838:1841	arg1	trait					1851:1855	T2DM complex trait	1838:1855	T2DM complex trait	1838:1855	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	1	61	theme	multiple	238:245	arg1	factors					287:293	multiple environmental, genomic, and postgenomic factors	238:293	multiple environmental, genomic, and postgenomic factors	238:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	4	62	theme	Western	834:840	arg1	Busselton					823:831	Busselton	823:831	Busselton	823:831	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	4	62	theme	Western	834:840	arg1	Australia					842:850	Western Australia	834:850	Western Australia	834:850	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	3	63	theme	postgenomic	673:683	arg1	factors					685:691	the genomic and postgenomic factors	657:691	the genomic and postgenomic factors	657:691	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	7	64	theme	area	1112:1115	arg1	analysis					1139:1146	area under the curve (AUC) analysis	1112:1146	area under the curve (AUC) analysis of the receiver operating characteristic curves	1112:1194	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	10	65	theme	95	1740:1741	arg1	%					1742:1742	%	1742:1742	%	1742:1742	The AUC for IgG glycans alone was 0.623 with a 95% confidence interval 0.580-0.666.					
31526239	5	66	theme	ultraperformance	868:883	arg1	method					907:912	the ultraperformance liquid chromatography method	864:912	the ultraperformance liquid chromatography method	864:912	We applied the ultraperformance liquid chromatography method to analyze the composition of IgG N-glycans.					
31526239	12	67	theme	proinflammatory	1973:1987	arg1	status					2010:2015	an increased proinflammatory and biological aging status	1960:2015	an increased proinflammatory and biological aging status	1960:2015	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	3	68	theme	co-	544:546	arg1	modifications					571:583	the most frequently observed co- and post-translational modifications	515:583	the most frequently observed co- and post-translational modifications	515:583	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	0	69	theme	Australian	125:134	arg1	Population					136:145	an Australian Population	122:145	an Australian Population	122:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	14	70	theme	cardiovascular	2341:2354	arg1	disease					2356:2362	cardiovascular disease	2341:2362	cardiovascular disease	2341:2362	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	10	71	theme	confidence	1744:1753	arg1	interval					1755:1762	a 95% confidence interval 0.580-0.666	1738:1774	a 95% confidence interval 0.580-0.666	1738:1774	The AUC for IgG glycans alone was 0.623 with a 95% confidence interval 0.580-0.666.					
31526239	1	72	theme	complex	192:198	arg1	mellitus					164:171	Type 2 diabetes mellitus	148:171	Type 2 diabetes mellitus (T2DM)	148:178	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	72	theme	complex	192:198	arg1	trait					200:204	a common complex trait	183:204	a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors	183:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	11	73	theme	new	1809:1811	arg1	evidence					1813:1820	new evidence	1809:1820	new evidence of diversity	1809:1833	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	3	74	theme	post-translational	552:569	arg1	modifications					571:583	the most frequently observed co- and post-translational modifications	515:583	the most frequently observed co- and post-translational modifications	515:583	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	5	75	theme	chromatography	892:905	arg1	method					907:912	the ultraperformance liquid chromatography method	864:912	the ultraperformance liquid chromatography method	864:912	We applied the ultraperformance liquid chromatography method to analyze the composition of IgG N-glycans.					
31526239	8	76	theme	IgG	1480:1482	arg1	traits					1493:1498	IgG N-glycan traits	1480:1498	IgG N-glycan traits	1480:1498	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	4	77	theme	community-based	699:713	arg1	study					728:732	a community-based case-control study	697:732	a community-based case-control study	697:732	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	14	78	theme	T2DM	2321:2324	arg1	progression					2326:2336	T2DM progression	2321:2336	T2DM progression	2321:2336	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	7	79	theme	clinical	1229:1236	arg1	factors					1243:1249	clinical risk factors	1229:1249	clinical risk factors	1229:1249	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	1	80	theme	Type	148:151	arg1	trait					200:204	a common complex trait	183:204	a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors	183:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	80	theme	Type	148:151	arg1	T2DM					174:177	T2DM	174:177	T2DM	174:177	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	80	theme	Type	148:151	arg1	mellitus					164:171	Type 2 diabetes mellitus	148:171	Type 2 diabetes mellitus (T2DM)	148:178	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	7	81	theme	fivefold	1199:1206	arg1	cross-validation					1208:1223	fivefold cross-validation	1199:1223	fivefold cross-validation for clinical risk factors, IgG glycans, and their combination	1199:1285	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	11	82	theme	complex	1843:1849	arg1	trait					1851:1855	T2DM complex trait	1838:1855	T2DM complex trait	1838:1855	In addition, our study provided new evidence of diversity in T2DM complex trait by IgG N-glycan stratification.					
31526239	12	83	theme	IgG	1893:1895	arg1	traits					1904:1909	Six IgG glycan traits	1889:1909	Six IgG glycan traits	1889:1909	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	1	84	theme	diabetes	155:162	arg1	trait					200:204	a common complex trait	183:204	a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors	183:293	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	84	theme	diabetes	155:162	arg1	T2DM					174:177	T2DM	174:177	T2DM	174:177	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	1	84	theme	diabetes	155:162	arg1	mellitus					164:171	Type 2 diabetes mellitus	148:171	Type 2 diabetes mellitus (T2DM)	148:178	Type 2 diabetes mellitus (T2DM) is a common complex trait arising from interactions among multiple environmental, genomic, and postgenomic factors.					
31526239	6	85	theme	correlation	988:998	arg1	analyses					1000:1007	Spearman's correlation analyses	977:1007	Spearman's correlation analyses	977:1007	We then conducted Spearman's correlation analyses to explore the association between glycan biomarker candidates and clinical risk factors.					
31526239	8	86	theme	derived	1319:1325	arg1	peaks					1334:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks	1288:1338	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	0	87	theme	Proinflammatory	92:106	arg1	Mechanisms					108:117	Putative Proinflammatory Mechanisms	83:117	Putative Proinflammatory Mechanisms in an Australian Population	83:145	Type 2 Diabetes Mellitus is Associated with the Immunoglobulin G N-Glycome through Putative Proinflammatory Mechanisms in an Australian Population.					
31526239	5	88	theme	N-glycans	948:956	arg1	composition					929:939	the composition	925:939	the composition of IgG N-glycans	925:956	We applied the ultraperformance liquid chromatography method to analyze the composition of IgG N-glycans.					
31526239	3	89	theme	observed	535:542	arg1	modifications					571:583	the most frequently observed co- and post-translational modifications	515:583	the most frequently observed co- and post-translational modifications	515:583	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	8	90	theme	clinical	1532:1539	arg1	factors					1546:1552	clinical risk factors	1532:1552	T2DM clinical risk factors	1527:1552	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	14	91	theme	T2DM	2287:2290	arg1	diagnosis					2292:2300	T2DM diagnosis	2287:2300	T2DM diagnosis	2287:2300	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	7	92	theme	characteristic	1174:1187	arg1	curves					1189:1194	characteristic curves	1174:1194	characteristic curves	1174:1194	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	6	93	theme	risk	1085:1088	arg1	factors					1090:1096	clinical risk factors	1076:1096	clinical risk factors	1076:1096	We then conducted Spearman's correlation analyses to explore the association between glycan biomarker candidates and clinical risk factors.					
31526239	3	94	gly	N-glycosylation	477:491	arg1	proteins					496:503	proteins	496:503	proteins	496:503	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	7	95	theme	receiver	1155:1162	arg1	analysis					1139:1146	area under the curve (AUC) analysis	1112:1146	area under the curve (AUC) analysis of the receiver operating characteristic curves	1112:1194	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	4	96	from	community	810:818	arg1	Busselton					823:831	Busselton	823:831	Busselton	823:831	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	4	96	from	community	810:818	arg1	Australia					842:850	Western Australia	834:850	Western Australia	834:850	In a community-based case-control study, 849 participants (217 cases and 632 controls) were recruited from an urban community in Busselton, Western Australia.					
31526239	14	97	theme	inflammatory	2249:2260	arg1	candidates					2272:2281	inflammatory biomarker candidates	2249:2281	inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure	2249:2379	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	14	97	theme	inflammatory	2249:2260	arg1	prospects					2236:2244	future prospects	2229:2244	future prospects	2229:2244	Furthermore, IgG N-glycomic alterations offer future prospects as inflammatory biomarker candidates for T2DM diagnosis, and monitoring of T2DM progression to cardiovascular disease or renal failure.					
31526239	2	98	theme	immunoglobulin	368:381	arg1	IgG					386:388	IgG	386:388	IgG	386:388	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	2	98	theme	immunoglobulin	368:381	arg1	G					383:383	immunoglobulin G	368:383	immunoglobulin G (IgG) N-glycan patterns	368:407	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	9	99	theme	glycan	1580:1585	arg1	profiles					1587:1594	the IgG glycan profiles	1572:1594	the IgG glycan profiles	1572:1594	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	9	100	from	glucose	1613:1619	arg1	model					1648:1652	the logistic regression model	1624:1652	the logistic regression model	1624:1652	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	6	101	theme	biomarker	1051:1059	arg1	candidates					1061:1070	glycan biomarker candidates	1044:1070	glycan biomarker candidates	1044:1070	We then conducted Spearman's correlation analyses to explore the association between glycan biomarker candidates and clinical risk factors.					
31526239	3	102	theme	real-time	615:623	arg1	status					625:630	the real-time status	611:630	the real-time status of the interplay between the genomic and postgenomic factors	611:691	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	9	103	theme	blood	1607:1611	arg1	glucose					1613:1619	fasting blood glucose	1599:1619	fasting blood glucose in the logistic regression model	1599:1652	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	2	104	theme	clinical	426:433	arg1	factors					440:446	their clinical risk factors	420:446	their clinical risk factors	420:446	We report here the first attempt to investigate the association between immunoglobulin G (IgG) N-glycan patterns, T2DM, and their clinical risk factors in an Australian population.					
31526239	8	105	theme	extensive	1402:1410	arg1	confounders					1421:1431	extensive clinical confounders	1402:1431	extensive clinical confounders	1402:1431	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	13	106	from	role	2147:2150	arg1	population					2119:2128	an Australian population	2105:2128	an Australian population	2105:2128	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	3	107	theme	interplay	639:647	arg1	status					625:630	the real-time status	611:630	the real-time status of the interplay between the genomic and postgenomic factors	611:691	N-glycosylation of proteins is one of the most frequently observed co- and post-translational modifications, reflecting, importantly, the real-time status of the interplay between the genomic and postgenomic factors.					
31526239	12	108	theme	aging	2004:2008	arg1	status					2010:2015	an increased proinflammatory and biological aging status	1960:2015	an increased proinflammatory and biological aging status	1960:2015	Six IgG glycan traits were firmly associated with T2DM, which reflects an increased proinflammatory and biological aging status.					
31526239	9	109	theme	logistic	1628:1635	arg1	model					1648:1652	the logistic regression model	1624:1652	the logistic regression model	1624:1652	Moreover, adding the IgG glycan profiles to fasting blood glucose in the logistic regression model increased the AUC from 0.799 to 0.859.					
31526239	7	110	theme	IgG	1252:1254	arg1	glycans					1256:1262	IgG glycans	1252:1262	IgG glycans	1252:1262	We performed area under the curve (AUC) analysis of the receiver operating characteristic curves by fivefold cross-validation for clinical risk factors, IgG glycans, and their combination.					
31526239	13	111	from	N-glycome	2083:2091	arg1	population					2119:2128	an Australian population	2105:2128	an Australian population	2105:2128	In summary, our study reports novel associations between the IgG N-glycome and T2DM in an Australian population and the putative role of proinflammatory mechanisms.					
31526239	8	112	theme	false	1437:1441	arg1	rate					1453:1456	false discovery rate	1437:1456	false discovery rate	1437:1456	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31526239	8	113	dep	T2DM	1527:1530	arg1	factors					1546:1552	clinical risk factors	1532:1552	T2DM clinical risk factors	1527:1552	Two directly measured and four derived glycan peaks were significantly associated with T2DM, after correction for extensive clinical confounders and false discovery rate, thus suggesting that IgG N-glycan traits are highly correlated with T2DM clinical risk factors.					
31851597	0	0	theme	cellular	96:103	arg1	adherence					105:113	cellular adherence	96:113	cellular adherence	96:113	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	2	1	link	O-linked	310:317	arg1	glycans					327:333	O-linked mannose glycans	310:333	O-linked mannose glycans	310:333	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	4	2	theme	engineered	744:753	arg1	cells					755:759	genetically engineered cells	732:759	genetically engineered cells lacking all four TMTC genes	732:787	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	6	3	theme	Cobblestone	1094:1104	arg1	lissencephaly					1106:1118	Cobblestone lissencephaly	1094:1118	Cobblestone lissencephaly	1094:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	7	4	theme	TMTC3	1133:1137	arg1	mutations					1139:1147	TMTC3 mutations	1133:1147	TMTC3 mutations associated with Cobblestone lissencephaly	1133:1189	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	2	5	theme	O-linked	523:530	arg1	mannoses					532:539	these O-linked mannoses	517:539	these O-linked mannoses	517:539	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	7	6	theme	gastrulation	1315:1326	arg1	rescue					1305:1310	TMTC3 rescue	1299:1310	TMTC3 rescue of gastrulation	1299:1326	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	4	7	theme	O-linked	822:829	arg1	glycosylation					831:843	O-linked glycosylation	822:843	O-linked glycosylation of E-cadherin and cell adherence	822:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	7	8	theme	Cobblestone	1165:1175	arg1	lissencephaly					1177:1189	Cobblestone lissencephaly	1165:1189	Cobblestone lissencephaly	1165:1189	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	2	9	theme	O-linked	310:317	arg1	glycans					327:333	O-linked mannose glycans	310:333	O-linked mannose glycans	310:333	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	0	10	theme	adherence	105:113	arg1	O-mannosylation					65:79	O-mannosylation	65:79	O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation	65:141	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	8	11	theme	cadherin-mediated	1430:1446	arg1	adherence					1448:1456	cadherin-mediated adherence	1430:1456	cadherin-mediated adherence	1430:1456	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	5	12	theme	TMTC3	1005:1009	arg1	addition					987:994	the addition	983:994	the addition of human TMTC3	983:1009	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	0	13	theme	embryonic	120:128	arg1	gastrulation					130:141	embryonic gastrulation	120:141	embryonic gastrulation	120:141	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	7	14	theme	reduced	1232:1238	arg1	stability					1240:1248	reduced stability	1232:1248	reduced stability	1232:1248	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	2	15	theme	molecules	294:302	arg1	superfamily					270:280	The cadherin superfamily	257:280	The cadherin superfamily of adhesion molecules	257:302	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	1	16	theme	protein	191:197	arg1	structure					199:207	protein structure	191:207	protein structure	191:207	Protein glycosylation plays essential roles in protein structure, stability, and activity such as cell adhesion.					
31851597	8	17	link	O-linked	1403:1410	arg1	glycosylation					1412:1424	O-linked glycosylation	1403:1424	O-linked glycosylation	1403:1424	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	2	18	theme	adhesion	285:292	arg1	molecules					294:302	adhesion molecules	285:302	adhesion molecules	285:302	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	8	19	theme	cellular	1496:1503	arg1	adherence					1505:1513	cellular adherence	1496:1513	cellular adherence	1496:1513	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	2	20	theme	tetratricopeptide	414:430	arg1	proteins					450:457	tetratricopeptide repeat-containing proteins	414:457	tetratricopeptide repeat-containing proteins	414:457	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	0	21	theme	reticulum	12:20	arg1	TMTC3					44:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	6	22	from	Mutations	1012:1020	arg1	TMTC3					1025:1029	TMTC3	1025:1029	TMTC3	1025:1029	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	7	23	theme	TMTC3	1299:1303	arg1	rescue					1305:1310	TMTC3 rescue	1299:1310	TMTC3 rescue of gastrulation	1299:1326	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	2	24	theme	conserved	338:346	arg1	sites					348:352	conserved sites	338:352	conserved sites	338:352	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	5	25	theme	laevis	917:922	arg1	embryos					924:930	Xenopus laevis embryos	909:930	Xenopus laevis embryos	909:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	0	26	theme	Endoplasmic	0:10	arg1	reticulum					12:20	Endoplasmic reticulum	0:20	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	8	27	theme	O-linked	1403:1410	arg1	glycosylation					1412:1424	O-linked glycosylation	1403:1424	O-linked glycosylation	1403:1424	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	7	28	theme	mutations	1139:1147	arg1	Analysis					1121:1128	Analysis	1121:1128	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly	1121:1189	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	4	29	theme	TMTC	778:781	arg1	genes					783:787	all four TMTC genes	769:787	all four TMTC genes	769:787	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	4	30	theme	TMTC3	808:812	arg1	overexpression					790:803	overexpression	790:803	overexpression of TMTC3	790:812	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	2	31	theme	gene	473:476	arg1	products					478:485	1-4 (TMTC1-4) gene products	459:485	1-4 (TMTC1-4) gene products	459:485	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	3	32	theme	organismal	582:591	arg1	analysis					593:600	biochemical, cell biological, and organismal analysis	548:600	biochemical, cell biological, and organismal analysis	548:600	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	5	33	from	delay	941:945	arg1	gastrulation					950:961	gastrulation	950:961	gastrulation	950:961	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	5	34	theme	human	999:1003	arg1	TMTC3					1005:1009	human TMTC3	999:1009	human TMTC3	999:1009	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	0	35	theme	transmembrane	22:34	arg1	TMTC3					44:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	2	36	contain	carry	304:308	arg3	sites					348:352	conserved sites	338:352	conserved sites	338:352	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	2	36	contain	carry	304:308	arg2	glycans					327:333	O-linked mannose glycans	310:333	O-linked mannose glycans	310:333	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	2	36	contain	carry	304:308	arg1	superfamily					270:280	The cadherin superfamily	257:280	The cadherin superfamily of adhesion molecules	257:302	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	0	37	theme	gastrulation	130:141	arg1	O-mannosylation					65:79	O-mannosylation	65:79	O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation	65:141	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	3	38	theme	cellular	679:686	arg1	adhesion					688:695	cellular adhesion	679:695	cellular adhesion	679:695	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	2	39	theme	mannose	319:325	arg1	glycans					327:333	O-linked mannose glycans	310:333	O-linked mannose glycans	310:333	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	5	40	theme	Xenopus	909:915	arg1	embryos					924:930	Xenopus laevis embryos	909:930	Xenopus laevis embryos	909:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	4	41	gly	glycosylation	831:843	arg1	E-cadherin					848:857	E-cadherin	848:857	E-cadherin	848:857	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	4	41	gly	glycosylation	831:843	arg1	adherence					868:876	cell adherence	863:876	cell adherence	863:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	6	42	theme	neuronal	1051:1058	arg1	diseases					1075:1082	neuronal cell migration diseases	1051:1082	neuronal cell migration diseases including Cobblestone lissencephaly	1051:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	42	theme	neuronal	1051:1058	arg1	lissencephaly					1106:1118	Cobblestone lissencephaly	1094:1118	Cobblestone lissencephaly	1094:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	3	43	theme	adhesion	688:695	arg1	O-mannosylation					648:662	the O-mannosylation	644:662	the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation	644:723	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	4	44	theme	adherence	868:876	arg1	glycosylation					831:843	O-linked glycosylation	822:843	O-linked glycosylation of E-cadherin and cell adherence	822:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	3	45	used	used	606:609	arg2	analysis					593:600	biochemical, cell biological, and organismal analysis	548:600	biochemical, cell biological, and organismal analysis	548:600	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	2	46	theme	repeat-containing	432:448	arg1	proteins					450:457	tetratricopeptide repeat-containing proteins	414:457	tetratricopeptide repeat-containing proteins	414:457	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	7	47	theme	variants	1215:1222	arg1	three					1202:1206	three	1202:1206	three	1202:1206	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	7	47	theme	variants	1215:1222	arg1	variants					1215:1222	the variants	1211:1222	the variants	1211:1222	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	3	48	theme	E-cadherin	667:676	arg1	O-mannosylation					648:662	the O-mannosylation	644:662	the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation	644:723	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	0	49	theme	protein	36:42	arg1	TMTC3					44:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3	0:48	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	2	50	link	O-linked	523:530	arg1	mannoses					532:539	these O-linked mannoses	517:539	these O-linked mannoses	517:539	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	5	51	theme	Tmtcs	900:904	arg1	knockdown					883:891	The knockdown	879:891	The knockdown of the Tmtcs in Xenopus laevis embryos	879:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	8	52	theme	lissencephaly	1580:1592	arg1	basis					1542:1546	well the basis	1533:1546	well the basis of TMTC3-associated Cobblestone lissencephaly	1533:1592	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	8	52	theme	lissencephaly	1580:1592	arg1	insight					1469:1475	insight	1469:1475	insight into its effect on cellular adherence and migration	1469:1527	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	5	53	from	Tmtcs	900:904	arg1	embryos					924:930	Xenopus laevis embryos	909:930	Xenopus laevis embryos	909:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	4	54	link	O-linked	822:829	arg1	glycosylation					831:843	O-linked glycosylation	822:843	O-linked glycosylation of E-cadherin and cell adherence	822:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	3	55	theme	embryonic	702:710	arg1	gastrulation					712:723	embryonic gastrulation	702:723	embryonic gastrulation	702:723	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	8	56	theme	Cobblestone	1568:1578	arg1	lissencephaly					1580:1592	TMTC3-associated Cobblestone lissencephaly	1551:1592	TMTC3-associated Cobblestone lissencephaly	1551:1592	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	5	57	from	knockdown	883:891	arg1	embryos					924:930	Xenopus laevis embryos	909:930	Xenopus laevis embryos	909:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	8	58	theme	TMTC3-associated	1551:1566	arg1	lissencephaly					1580:1592	TMTC3-associated Cobblestone lissencephaly	1551:1592	TMTC3-associated Cobblestone lissencephaly	1551:1592	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	4	59	theme	E-cadherin	848:857	arg1	glycosylation					831:843	O-linked glycosylation	822:843	O-linked glycosylation of E-cadherin and cell adherence	822:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	3	60	theme	gastrulation	712:723	arg1	O-mannosylation					648:662	the O-mannosylation	644:662	the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation	644:723	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	4	61	theme	cell	863:866	arg1	adherence					868:876	cell adherence	863:876	cell adherence	863:876	Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence.					
31851597	1	62	theme	Protein	144:150	arg1	glycosylation					152:164	Protein glycosylation	144:164	Protein glycosylation	144:164	Protein glycosylation plays essential roles in protein structure, stability, and activity such as cell adhesion.					
31851597	6	63	attach	linked	1041:1046	arg2	Mutations					1012:1020	Mutations	1012:1020	Mutations in TMTC3	1012:1029	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	63	attach	linked	1041:1046	arg1	diseases					1075:1082	neuronal cell migration diseases	1051:1082	neuronal cell migration diseases including Cobblestone lissencephaly	1051:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	63	attach	linked	1041:1046	arg1	lissencephaly					1106:1118	Cobblestone lissencephaly	1094:1118	Cobblestone lissencephaly	1094:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	64	theme	cell	1060:1063	arg1	diseases					1075:1082	neuronal cell migration diseases	1051:1082	neuronal cell migration diseases including Cobblestone lissencephaly	1051:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	64	theme	cell	1060:1063	arg1	lissencephaly					1106:1118	Cobblestone lissencephaly	1094:1118	Cobblestone lissencephaly	1094:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	3	65	theme	biological	566:575	arg1	analysis					593:600	biochemical, cell biological, and organismal analysis	548:600	biochemical, cell biological, and organismal analysis	548:600	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	2	66	dep	transmembrane	396:408	arg1	products					478:485	1-4 (TMTC1-4) gene products	459:485	1-4 (TMTC1-4) gene products	459:485	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	7	67	theme	Xenopus	1331:1337	arg1	development					1346:1356	Xenopus embryo development	1331:1356	Xenopus embryo development	1331:1356	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	1	68	theme	cell	242:245	arg1	adhesion					247:254	cell adhesion	242:254	cell adhesion	242:254	Protein glycosylation plays essential roles in protein structure, stability, and activity such as cell adhesion.					
31851597	0	69	theme	E-cadherin	84:93	arg1	O-mannosylation					65:79	O-mannosylation	65:79	O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation	65:141	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	6	70	theme	migration	1065:1073	arg1	diseases					1075:1082	neuronal cell migration diseases	1051:1082	neuronal cell migration diseases including Cobblestone lissencephaly	1051:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	6	70	theme	migration	1065:1073	arg1	lissencephaly					1106:1118	Cobblestone lissencephaly	1094:1118	Cobblestone lissencephaly	1094:1118	Mutations in TMTC3 have been linked to neuronal cell migration diseases including Cobblestone lissencephaly.					
31851597	2	71	theme	cadherin	261:268	arg1	superfamily					270:280	The cadherin superfamily	257:280	The cadherin superfamily of adhesion molecules	257:302	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	5	72	from	embryos	924:930	arg1	knockdown					883:891	The knockdown	879:891	The knockdown of the Tmtcs in Xenopus laevis embryos	879:930	The knockdown of the Tmtcs in Xenopus laevis embryos caused a delay in gastrulation that was rescued by the addition of human TMTC3.					
31851597	3	73	theme	biochemical	548:558	arg1	analysis					593:600	biochemical, cell biological, and organismal analysis	548:600	biochemical, cell biological, and organismal analysis	548:600	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	3	74	gly	O-mannosylation	648:662	arg1	E-cadherin					667:676	E-cadherin	667:676	E-cadherin	667:676	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	3	74	gly	O-mannosylation	648:662	arg1	adhesion					688:695	cellular adhesion	679:695	cellular adhesion	679:695	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	3	74	gly	O-mannosylation	648:662	arg1	gastrulation					712:723	embryonic gastrulation	702:723	embryonic gastrulation	702:723	Here, biochemical, cell biological, and organismal analysis was used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embryonic gastrulation.					
31851597	2	75	theme	mannoses	532:539	arg1	addition					505:512	the addition	501:512	the addition of these O-linked mannoses	501:539	The cadherin superfamily of adhesion molecules carry O-linked mannose glycans at conserved sites and it was recently demonstrated that the transmembrane and tetratricopeptide repeat-containing proteins 1-4 (TMTC1-4) gene products contribute to the addition of these O-linked mannoses.					
31851597	7	76	theme	missence	1254:1261	arg1	mutations					1263:1271	missence mutations	1254:1271	missence mutations	1254:1271	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	0	77	gly	O-mannosylation	65:79	arg1	gastrulation					130:141	embryonic gastrulation	120:141	embryonic gastrulation	120:141	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	0	77	gly	O-mannosylation	65:79	arg1	E-cadherin					84:93	E-cadherin	84:93	E-cadherin	84:93	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	0	77	gly	O-mannosylation	65:79	arg1	adherence					105:113	cellular adherence	96:113	cellular adherence	96:113	Endoplasmic reticulum transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, cellular adherence, and embryonic gastrulation.					
31851597	7	78	theme	embryo	1339:1344	arg1	development					1346:1356	Xenopus embryo development	1331:1356	Xenopus embryo development	1331:1356	Analysis of TMTC3 mutations associated with Cobblestone lissencephaly found that three of the variants exhibit reduced stability and missence mutations were unable to complement TMTC3 rescue of gastrulation in Xenopus embryo development.					
31851597	1	79	theme	essential	172:180	arg1	roles					182:186	essential roles	172:186	essential roles	172:186	Protein glycosylation plays essential roles in protein structure, stability, and activity such as cell adhesion.					
31851597	8	80	from	effect	1486:1491	arg1	adherence					1505:1513	cellular adherence	1496:1513	cellular adherence	1496:1513	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
31851597	8	80	from	effect	1486:1491	arg1	migration					1519:1527	migration	1519:1527	migration	1519:1527	Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly.					
29578022	4	0	theme	polysaccharides	698:712	arg1	%					684:684	63%	682:684	63% of sulfated polysaccharides	682:712	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	4	0	theme	polysaccharides	698:712	arg1	polysaccharides					698:712	sulfated polysaccharides	689:712	sulfated polysaccharides	689:712	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	1	1	theme	major	147:151	arg1	Autolysis					123:131	Autolysis	123:131	Autolysis	123:131	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	1	1	theme	major	147:151	arg1	reason					153:158	a major reason	145:158	a major reason for postharvest quality deterioration of sea cucumber	145:212	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	6	2	gly	fucosylated	1003:1013	arg1	sulfate					1027:1033	fucosylated chondroitin sulfate	1003:1033	fucosylated chondroitin sulfate ratio	1003:1039	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	7	3	theme	possible	1304:1311	arg1	changes					1313:1319	possible changes	1304:1319	possible changes on their bioactivities	1304:1342	To conclude, A-SP differed greatly from T-SP in the chemical composition, suggesting possible changes on their bioactivities.					
29578022	0	4	from	analysis	14:21	arg1	cucumber					60:67	sea cucumber	56:67	sea cucumber (Stichopus japonicus)	56:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	4	5	theme	Chemical	627:634	arg1	analysis					636:643	Chemical analysis	627:643	Chemical analysis	627:643	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	4	6	theme	sulfated	689:696	arg1	polysaccharides					698:712	sulfated polysaccharides	689:712	sulfated polysaccharides	689:712	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	6	7	theme	molecular	1101:1109	arg1	weight					1111:1116	a lower molecular weight	1093:1116	a lower molecular weight	1093:1116	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	1	8	theme	exogenous	252:260	arg1	protease					262:269	exogenous protease	252:269	exogenous protease	252:269	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	5	9	theme	analysis	892:899	arg1	HPLC					937:940	HPLC	937:940	HPLC	937:940	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	9	theme	analysis	892:899	arg1	FTIR					923:926	FTIR	923:926	FTIR	923:926	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	9	theme	analysis	892:899	arg1	techniques					901:910	analysis techniques	892:910	analysis techniques	892:910	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	9	theme	analysis	892:899	arg1	spectroscopy					951:962	mass spectroscopy	946:962	mass spectroscopy	946:962	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	9	theme	analysis	892:899	arg1	NMR					932:934	1H NMR	929:934	1H NMR	929:934	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	0	10	from	cucumber	60:67	arg1	analysis					14:21	Compositional analysis	0:21	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus)	0:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	0	10	from	cucumber	60:67	arg1	polysaccharides					35:49	sulfated polysaccharides	26:49	sulfated polysaccharides from sea cucumber (Stichopus japonicus)	26:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	4	11	theme	reducing	762:769	arg1	ends					771:774	their reducing ends	756:774	their reducing ends	756:774	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	3	12	theme	chemical	540:547	arg1	composition					549:559	the chemical composition	536:559	the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction	536:624	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	3	13	theme	autolysis	487:495	arg1	reaction					497:504	autolysis reaction	487:504	autolysis reaction	487:504	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	6	14	theme	Fuc	1170:1172	arg1	absent					1203:1208	absent	1203:1208	absent	1203:1208	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	14	theme	Fuc	1170:1172	arg1	residue					1174:1180	the di-sulfated Fuc residue	1154:1180	the di-sulfated Fuc residue observed in T-SP	1154:1197	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	2	15	theme	cucumber	409:416	arg1	polysaccharides					386:400	bioactive polysaccharides	376:400	bioactive polysaccharides of sea cucumber	376:416	However, little has been known about the effects of autolysis on bioactive polysaccharides of sea cucumber.					
29578022	5	16	theme	1H	929:930	arg1	techniques					901:910	analysis techniques	892:910	analysis techniques	892:910	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	16	theme	1H	929:930	arg1	FTIR					923:926	FTIR	923:926	FTIR	923:926	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	16	theme	1H	929:930	arg1	NMR					932:934	1H NMR	929:934	1H NMR	929:934	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	4	17	theme	3-day	666:670	arg1	autolysis					672:680	3-day autolysis	666:680	3-day autolysis	666:680	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	0	18	theme	autolysis	103:111	arg1	reaction					113:120	autolysis reaction	103:120	autolysis reaction	103:120	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	6	19	theme	di-sulfated	1158:1168	arg1	absent					1203:1208	absent	1203:1208	absent	1203:1208	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	19	theme	di-sulfated	1158:1168	arg1	residue					1174:1180	the di-sulfated Fuc residue	1154:1180	the di-sulfated Fuc residue observed in T-SP	1154:1197	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	20	theme	sulfate	1027:1033	arg1	ratio					1035:1039	fucosylated chondroitin sulfate ratio	1003:1039	fucosylated chondroitin sulfate ratio	1003:1039	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	2	21	theme	bioactive	376:384	arg1	polysaccharides					386:400	bioactive polysaccharides	376:400	bioactive polysaccharides of sea cucumber	376:416	However, little has been known about the effects of autolysis on bioactive polysaccharides of sea cucumber.					
29578022	6	22	theme	chondroitin	1015:1025	arg1	sulfate					1027:1033	fucosylated chondroitin sulfate	1003:1033	fucosylated chondroitin sulfate ratio	1003:1039	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	3	23	theme	sulfated	564:571	arg1	SPs					590:592	SPs	590:592	SPs	590:592	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	3	23	theme	sulfated	564:571	arg1	polysaccharides					573:587	sulfated polysaccharides	564:587	sulfated polysaccharides (SPs) released by autolysis reaction	564:624	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	1	24	theme	quality	176:182	arg1	deterioration					184:196	postharvest quality deterioration	164:196	postharvest quality deterioration of sea cucumber	164:212	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	0	25	theme	Compositional	0:12	arg1	analysis					14:21	Compositional analysis	0:21	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus)	0:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	3	26	theme	polysaccharides	573:587	arg1	composition					549:559	the chemical composition	536:559	the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction	536:624	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	2	27	theme	autolysis	363:371	arg1	effects					352:358	the effects	348:358	the effects of autolysis on bioactive polysaccharides of sea cucumber	348:416	However, little has been known about the effects of autolysis on bioactive polysaccharides of sea cucumber.					
29578022	0	28	theme	sulfated	26:33	arg1	polysaccharides					35:49	sulfated polysaccharides	26:49	sulfated polysaccharides from sea cucumber (Stichopus japonicus)	26:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	6	29	theme	fucosylated	1003:1013	arg1	sulfate					1027:1033	fucosylated chondroitin sulfate	1003:1033	fucosylated chondroitin sulfate ratio	1003:1039	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	2	30	from	effects	352:358	arg1	polysaccharides					386:400	bioactive polysaccharides	376:400	bioactive polysaccharides of sea cucumber	376:416	However, little has been known about the effects of autolysis on bioactive polysaccharides of sea cucumber.					
29578022	3	31	theme	sea	456:458	arg1	products					469:476	sea cucumber products	456:476	sea cucumber products involved autolysis reaction	456:504	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	4	32	theme	protein	738:744	arg1	chains					746:751	protein chains	738:751	protein chains	738:751	Chemical analysis indicated that after 3-day autolysis 63% of sulfated polysaccharides were liberated but with protein chains at their reducing ends.					
29578022	5	33	theme	techniques	901:910	arg1	series					882:887	a series	880:887	a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy	880:962	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	6	34	located	observed	1182:1189	arg2	residue					1174:1180	the di-sulfated Fuc residue	1154:1180	the di-sulfated Fuc residue observed in T-SP	1154:1197	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	34	located	observed	1182:1189	arg2	absent					1203:1208	absent	1203:1208	absent	1203:1208	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	34	located	observed	1182:1189	arg1	T-SP					1194:1197	T-SP	1194:1197	T-SP	1194:1197	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	1	35	theme	sea	201:203	arg1	cucumber					205:212	sea cucumber	201:212	sea cucumber	201:212	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	1	36	theme	cucumber	205:212	arg1	deterioration					184:196	postharvest quality deterioration	164:196	postharvest quality deterioration of sea cucumber	164:212	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	0	37	dep	cucumber	60:67	arg1	japonicus					80:88	Stichopus japonicus	70:88	Stichopus japonicus	70:88	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	5	38	theme	total	859:863	arg1	T-SP					870:873	T-SP	870:873	T-SP	870:873	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	38	theme	total	859:863	arg1	SPs					865:867	total SPs	859:867	total SPs (T-SP)	859:874	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	2	39	theme	sea	405:407	arg1	cucumber					409:416	sea cucumber	405:416	sea cucumber	405:416	However, little has been known about the effects of autolysis on bioactive polysaccharides of sea cucumber.					
29578022	0	40	theme	polysaccharides	35:49	arg1	analysis					14:21	Compositional analysis	0:21	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus)	0:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	6	41	from	absent	1203:1208	arg1	A-SP					1213:1216	A-SP	1213:1216	A-SP	1213:1216	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	7	42	theme	chemical	1271:1278	arg1	composition					1280:1290	the chemical composition	1267:1290	the chemical composition	1267:1290	To conclude, A-SP differed greatly from T-SP in the chemical composition, suggesting possible changes on their bioactivities.					
29578022	5	43	theme	SP	801:802	arg1	composition					786:796	Then the composition	777:796	Then the composition of SP obtained by autolysis (A-SP)	777:831	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	3	44	dep	quality	434:440	arg1	the					430:432	the	430:432	the	430:432	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	3	45	theme	cucumber	460:467	arg1	products					469:476	sea cucumber products	456:476	sea cucumber products involved autolysis reaction	456:504	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	0	46	theme	sea	56:58	arg1	cucumber					60:67	sea cucumber	56:67	sea cucumber (Stichopus japonicus)	56:89	Compositional analysis of sulfated polysaccharides from sea cucumber (Stichopus japonicus) released by autolysis reaction.					
29578022	6	47	theme	lower	1095:1099	arg1	weight					1111:1116	a lower molecular weight	1093:1116	a lower molecular weight	1093:1116	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	5	48	theme	mass	946:949	arg1	FTIR					923:926	FTIR	923:926	FTIR	923:926	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	48	theme	mass	946:949	arg1	techniques					901:910	analysis techniques	892:910	analysis techniques	892:910	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	5	48	theme	mass	946:949	arg1	spectroscopy					951:962	mass spectroscopy	946:962	mass spectroscopy	946:962	Then the composition of SP obtained by autolysis (A-SP) was compared with that of total SPs (T-SP) via a series of analysis techniques, including FTIR, 1H NMR, HPLC and mass spectroscopy.					
29578022	6	49	theme	major	1126:1130	arg1	fucan					1082:1086	fucan	1082:1086	fucan with a lower molecular weight	1082:1116	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	49	theme	major	1126:1130	arg1	fraction					1132:1139	the major fraction	1122:1139	the major fraction in A-SP	1122:1147	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	3	50	theme	products	469:476	arg1	quality					434:440	quality	434:440	quality	434:440	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	3	50	theme	products	469:476	arg1	safety					446:451	safety	446:451	safety	446:451	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	6	51	from	fraction	1132:1139	arg1	A-SP					1144:1147	A-SP	1144:1147	A-SP	1144:1147	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	52	from	A-SP	1213:1216	arg1	absent					1203:1208	absent	1203:1208	absent	1203:1208	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	6	52	from	A-SP	1213:1216	arg1	residue					1174:1180	the di-sulfated Fuc residue	1154:1180	the di-sulfated Fuc residue observed in T-SP	1154:1197	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	3	53	theme	present	511:517	arg1	study					519:523	the present study	507:523	the present study	507:523	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	1	54	theme	postharvest	164:174	arg1	deterioration					184:196	postharvest quality deterioration	164:196	postharvest quality deterioration of sea cucumber	164:212	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29578022	6	55	with	fucan	1082:1086	arg1	weight					1111:1116	a lower molecular weight	1093:1116	a lower molecular weight	1093:1116	As indicated by the results, fucan to fucosylated chondroitin sulfate ratio was found high in A-SP compared to T-SP, fucan with a lower molecular weight was the major fraction in A-SP, and the di-sulfated Fuc residue observed in T-SP was absent in A-SP.					
29578022	3	56	theme	autolysis	607:615	arg1	reaction					617:624	autolysis reaction	607:624	autolysis reaction	607:624	Concerning the quality and safety of sea cucumber products involved autolysis reaction, the present study focused on the chemical composition of sulfated polysaccharides (SPs) released by autolysis reaction.					
29578022	7	57	from	changes	1313:1319	arg1	bioactivities					1330:1342	their bioactivities	1324:1342	their bioactivities	1324:1342	To conclude, A-SP differed greatly from T-SP in the chemical composition, suggesting possible changes on their bioactivities.					
29578022	1	58	theme	promising	226:234	arg1	alternative					236:246	a promising alternative	224:246	a promising alternative for exogenous protease to produce peptides or polysaccharides	224:308	Autolysis is not only a major reason for postharvest quality deterioration of sea cucumber, but also a promising alternative for exogenous protease to produce peptides or polysaccharides.					
29751008	7	0	theme	receptor	1371:1378	arg1	properties					1388:1397	their FcγRIIIa receptor binding properties	1356:1397	their FcγRIIIa receptor binding properties	1356:1397	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	1	1	theme	Pichia	293:298	arg1	pastoris					300:307	Pichia pastoris	293:307	Pichia pastoris	293:307	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	3	2	theme	glycan	785:790	arg1	structure					792:800	glycan structure	785:800	glycan structure	785:800	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	1	3	theme	local	201:205	arg1	flexibility					216:226	local backbone flexibility	201:226	local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments	201:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	4	used	used	144:147	arg2	We					136:137	We	136:137	We	136:137	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	3	5	theme	pharmaceutical	812:825	arg1	properties					827:836	these pharmaceutical properties	806:836	these pharmaceutical properties	806:836	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	6	theme	results	610:616	arg1	Comparison					590:599	Comparison	590:599	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins	590:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	8	7	theme	well-defined	1406:1417	arg1	system					1462:1467	a useful model system	1447:1467	a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1447:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	7	theme	well-defined	1406:1417	arg1	glycoforms					1427:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	2	8	theme	backbone	568:575	arg1	flexibility					577:587	backbone flexibility	568:587	backbone flexibility	568:587	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	8	9	gly	glycoforms	1595:1604	arg1	IgG					1591:1593	various IgG glycoforms	1583:1604	various IgG glycoforms for potential applicability to future comparability	1583:1656	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	3	10	theme	physicochemical	642:656	arg1	stability					658:666	physicochemical stability	642:666	physicochemical stability	642:666	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	1	11	theme	IgG1-Fc	246:252	arg1	glycoforms					254:263	4 well-defined IgG1-Fc glycoforms	231:263	4 well-defined IgG1-Fc glycoforms	231:263	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	8	12	theme	future	1637:1642	arg1	comparability					1644:1656	future comparability	1637:1656	future comparability	1637:1656	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	13	theme	useful	1449:1454	arg1	system					1462:1467	a useful model system	1447:1467	a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1447:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	13	theme	useful	1449:1454	arg1	glycoforms					1427:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	3	14	theme	receptor	676:683	arg1	binding					685:691	Fcγ receptor binding	672:691	Fcγ receptor binding	672:691	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	6	15	theme	tryptophan	1137:1146	arg1	oxidation					1124:1132	oxidation	1124:1132	oxidation of tryptophan 277	1124:1150	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	7	16	theme	FcγRIIIa	1362:1369	arg1	properties					1388:1397	their FcγRIIIa receptor binding properties	1356:1397	their FcγRIIIa receptor binding properties	1356:1397	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	1	17	theme	enzymatic	361:369	arg1	treatments					371:380	subsequent in vitro enzymatic treatments	341:380	subsequent in vitro enzymatic treatments	341:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	5	18	theme	increased	991:999	arg1	flexibility					1016:1026	increased local backbone flexibility	991:1026	increased local backbone flexibility	991:1026	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	0	19	theme	IgG1-Fc	74:80	arg1	Glycoforms					82:91	Well-Defined IgG1-Fc Glycoforms	61:91	Well-Defined IgG1-Fc Glycoforms	61:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	5	20	theme	backbone	1007:1014	arg1	flexibility					1016:1026	increased local backbone flexibility	991:1026	increased local backbone flexibility	991:1026	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	0	21	from	Impact	0:5	arg1	Flexibility					46:56	the Local Backbone Flexibility	27:56	the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms	27:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	8	22	theme	stability	1497:1505	arg1	sets					1543:1546	physicochemical stability and local backbone flexibility data sets	1481:1546	physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1481:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	23	theme	local	1511:1515	arg1	flexibility					1526:1536	local backbone flexibility	1511:1536	local backbone flexibility	1511:1536	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	2	24	theme	oligosaccharide	438:452	arg1	size					412:415	the size	408:415	the size of the N-linked N297 oligosaccharide	408:452	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	0	25	theme	Exchange-Mass	108:120	arg1	Spectrometry					122:133	Hydrogen Exchange-Mass Spectrometry	99:133	Hydrogen Exchange-Mass Spectrometry	99:133	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	0	26	theme	Local	31:35	arg1	Flexibility					46:56	the Local Backbone Flexibility	27:56	the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms	27:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	6	27	theme	local	1201:1205	arg1	flexibility					1216:1226	decreased local backbone flexibility	1191:1226	decreased local backbone flexibility	1191:1226	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	8	28	theme	flexibility	1526:1536	arg1	sets					1543:1546	physicochemical stability and local backbone flexibility data sets	1481:1546	physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1481:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	2	29	theme	N-linked	424:431	arg1	oligosaccharide					438:452	the N-linked N297 oligosaccharide	420:452	the N-linked N297 oligosaccharide	420:452	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	7	30	theme	glycan	1310:1315	arg1	size					1317:1320	glycan size	1310:1320	glycan size	1310:1320	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	0	31	theme	Glycosylation	10:22	arg1	Impact					0:5	Impact	0:5	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms	0:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	4	32	theme	potential	903:911	arg1	regions					931:937	2 potential aggregation-prone regions	901:937	2 potential aggregation-prone regions	901:937	Flexibility significantly increased upon glycan truncation in 2 potential aggregation-prone regions.					
29751008	2	33	theme	high	459:462	arg1	Man8-Man12					473:482	Man8-Man12	473:482	Man8-Man12	473:482	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	2	33	theme	high	459:462	arg1	mannose					464:470	high mannose	459:470	high mannose (Man8-Man12)	459:483	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	7	34	theme	loop	1277:1280	arg1	flexibility					1282:1292	C'E glycopeptide loop flexibility	1260:1292	C'E glycopeptide loop flexibility	1260:1292	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	8	35	theme	various	1583:1589	arg1	glycoforms					1595:1604	various IgG glycoforms	1583:1604	various IgG glycoforms for potential applicability to future comparability	1583:1656	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	0	36	theme	Backbone	37:44	arg1	Flexibility					46:56	the Local Backbone Flexibility	27:56	the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms	27:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	1	37	theme	subsequent	341:350	arg1	treatments					371:380	subsequent in vitro enzymatic treatments	341:380	subsequent in vitro enzymatic treatments	341:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	3	38	theme	glycoproteins	719:731	arg1	set					712:714	the same set	703:714	the same set of glycoproteins	703:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	38	theme	glycoproteins	719:731	arg1	glycoproteins					719:731	glycoproteins	719:731	glycoproteins	719:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	4	39	theme	glycan	880:885	arg1	truncation					887:896	glycan truncation	880:896	glycan truncation	880:896	Flexibility significantly increased upon glycan truncation in 2 potential aggregation-prone regions.					
29751008	1	40	theme	glycoforms	254:263	arg1	2					310:310	2	310:310	2	310:310	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	40	theme	glycoforms	254:263	arg1	flexibility					216:226	local backbone flexibility	201:226	local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments	201:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	40	theme	glycoforms	254:263	arg1	glycoforms					254:263	4 well-defined IgG1-Fc glycoforms	231:263	4 well-defined IgG1-Fc glycoforms	231:263	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	41	theme	exchange-mass	158:170	arg1	spectrometry					172:183	hydrogen exchange-mass spectrometry	149:183	hydrogen exchange-mass spectrometry	149:183	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	8	42	theme	potential	1610:1618	arg1	applicability					1620:1632	potential applicability	1610:1632	potential applicability to future comparability	1610:1656	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	5	43	from	asparagine	1057:1066	arg1	flexibility					1016:1026	increased local backbone flexibility	991:1026	increased local backbone flexibility	991:1026	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	5	43	from	asparagine	1057:1066	arg1	deamidation					1042:1052	increased deamidation	1032:1052	increased deamidation at asparagine 315	1032:1070	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	7	44	theme	binding	1380:1386	arg1	properties					1388:1397	their FcγRIIIa receptor binding properties	1356:1397	their FcγRIIIa receptor binding properties	1356:1397	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	3	45	theme	improved	741:748	arg1	insights					750:757	improved insights	741:757	improved insights into correlations between glycan structure and these pharmaceutical properties	741:836	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	7	46	theme	E	1262:1262	arg1	flexibility					1282:1292	C'E glycopeptide loop flexibility	1260:1292	C'E glycopeptide loop flexibility	1260:1292	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	1	47	theme	backbone	207:214	arg1	flexibility					216:226	local backbone flexibility	201:226	local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments	201:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	6	48	theme	opposite	1092:1099	arg1	trend					1101:1105	the opposite trend	1088:1105	the opposite trend	1088:1105	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	3	49	with	data	693:696	arg1	set					712:714	the same set	703:714	the same set of glycoproteins	703:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	49	with	data	693:696	arg1	glycoproteins					719:731	glycoproteins	719:731	glycoproteins	719:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	50	theme	published	632:640	arg1	data					693:696	recently published physicochemical stability and Fcγ receptor binding data	623:696	recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins	623:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	1	51	theme	well-defined	233:244	arg1	glycoforms					254:263	4 well-defined IgG1-Fc glycoforms	231:263	4 well-defined IgG1-Fc glycoforms	231:263	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	52	dep	glycoforms	254:263	arg1	purified					279:286	purified	279:286	purified from Pichia pastoris	279:307	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	1	52	dep	glycoforms	254:263	arg1	expressed					265:273	expressed	265:273	expressed	265:273	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	8	53	theme	IgG1-Fc	1419:1425	arg1	system					1462:1467	a useful model system	1447:1467	a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1447:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	53	theme	IgG1-Fc	1419:1425	arg1	glycoforms					1427:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	54	theme	biosimilarity	1661:1673	arg1	assessments					1675:1685	biosimilarity assessments	1661:1685	biosimilarity assessments	1661:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	3	55	theme	Fcγ	672:674	arg1	binding					685:691	Fcγ receptor binding	672:691	Fcγ receptor binding	672:691	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	5	56	theme	increased	1032:1040	arg1	deamidation					1042:1052	increased deamidation	1032:1052	increased deamidation at asparagine 315	1032:1070	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	2	57	from	increases	555:563	arg1	flexibility					577:587	backbone flexibility	568:587	backbone flexibility	568:587	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	0	58	theme	Well-Defined	61:72	arg1	Glycoforms					82:91	Well-Defined IgG1-Fc Glycoforms	61:91	Well-Defined IgG1-Fc Glycoforms	61:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	8	59	gly	glycoforms	1427:1436	arg1	IgG1-Fc					1419:1425	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	3	60	theme	stability	658:666	arg1	data					693:696	recently published physicochemical stability and Fcγ receptor binding data	623:696	recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins	623:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	1	61	theme	hydrogen	149:156	arg1	spectrometry					172:183	hydrogen exchange-mass spectrometry	149:183	hydrogen exchange-mass spectrometry	149:183	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	2	62	theme	nonglycosylated	509:523	arg1	N297Q					525:529	nonglycosylated N297Q	509:529	nonglycosylated N297Q	509:529	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	8	63	theme	model	1456:1460	arg1	system					1462:1467	a useful model system	1447:1467	a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1447:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	8	63	theme	model	1456:1460	arg1	glycoforms					1427:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms	1400:1436	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	0	64	theme	Glycoforms	82:91	arg1	Flexibility					46:56	the Local Backbone Flexibility	27:56	the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms	27:91	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	5	65	theme	local	1001:1005	arg1	flexibility					1016:1026	increased local backbone flexibility	991:1026	increased local backbone flexibility	991:1026	In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315.					
29751008	0	66	theme	Hydrogen	99:106	arg1	Spectrometry					122:133	Hydrogen Exchange-Mass Spectrometry	99:133	Hydrogen Exchange-Mass Spectrometry	99:133	Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.					
29751008	1	67	gly	glycoforms	254:263	arg1	IgG1-Fc					246:252	4 well-defined IgG1-Fc glycoforms	231:263	4 well-defined IgG1-Fc glycoforms	231:263	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	2	68	theme	progressive	543:553	arg1	increases					555:563	progressive increases	543:563	progressive increases in backbone flexibility	543:587	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	8	69	theme	physicochemical	1481:1495	arg1	stability					1497:1505	physicochemical stability	1481:1505	physicochemical stability	1481:1505	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	6	70	theme	backbone	1207:1214	arg1	flexibility					1216:1226	decreased local backbone flexibility	1191:1226	decreased local backbone flexibility	1191:1226	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	2	71	theme	N297	433:436	arg1	oligosaccharide					438:452	the N-linked N297 oligosaccharide	420:452	the N-linked N297 oligosaccharide	420:452	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	3	72	with	Comparison	590:599	arg1	data					693:696	recently published physicochemical stability and Fcγ receptor binding data	623:696	recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins	623:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	2	73	gly	nonglycosylated	509:523	arg1	N297Q					525:529	nonglycosylated N297Q	509:529	nonglycosylated N297Q	509:529	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	6	74	theme	decreased	1191:1199	arg1	flexibility					1216:1226	decreased local backbone flexibility	1191:1226	decreased local backbone flexibility	1191:1226	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	8	75	theme	backbone	1517:1524	arg1	flexibility					1526:1536	local backbone flexibility	1511:1536	local backbone flexibility	1511:1536	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	2	76	link	N-linked	424:431	arg1	oligosaccharide					438:452	the N-linked N297 oligosaccharide	420:452	the N-linked N297 oligosaccharide	420:452	Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility.					
29751008	8	77	theme	data	1538:1541	arg1	sets					1543:1546	physicochemical stability and local backbone flexibility data sets	1481:1546	physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments	1481:1685	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
29751008	7	78	theme	glycopeptide	1264:1275	arg1	flexibility					1282:1292	C'E glycopeptide loop flexibility	1260:1292	C'E glycopeptide loop flexibility	1260:1292	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	6	79	theme	faster	1158:1163	arg1	oxidation					1165:1173	faster oxidation	1158:1173	faster oxidation	1158:1173	Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility.					
29751008	3	80	theme	binding	685:691	arg1	data					693:696	recently published physicochemical stability and Fcγ receptor binding data	623:696	recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins	623:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	7	81	gly	glycopeptide	1264:1275	arg2	glycopeptide					1264:1275	C'E glycopeptide loop flexibility	1260:1292	C'E glycopeptide loop flexibility	1260:1292	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	7	82	theme	C'	1260:1261	arg1	flexibility					1282:1292	C'E glycopeptide loop flexibility	1260:1292	C'E glycopeptide loop flexibility	1260:1292	Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties.					
29751008	4	83	theme	aggregation-prone	913:929	arg1	regions					931:937	2 potential aggregation-prone regions	901:937	2 potential aggregation-prone regions	901:937	Flexibility significantly increased upon glycan truncation in 2 potential aggregation-prone regions.					
29751008	1	84	theme	in vitro	352:359	arg1	treatments					371:380	subsequent in vitro enzymatic treatments	341:380	subsequent in vitro enzymatic treatments	341:380	We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments.					
29751008	3	85	gly	glycoproteins	719:731	arg1	glycoproteins					719:731	glycoproteins	719:731	glycoproteins	719:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	86	theme	same	707:710	arg1	set					712:714	the same set	703:714	the same set of glycoproteins	703:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	3	86	theme	same	707:710	arg1	glycoproteins					719:731	glycoproteins	719:731	glycoproteins	719:731	Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties.					
29751008	8	87	theme	IgG	1591:1593	arg1	glycoforms					1595:1604	various IgG glycoforms	1583:1604	various IgG glycoforms for potential applicability to future comparability	1583:1656	These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.					
31468229	0	0	theme	composition	80:90	arg1	analysis					92:99	related N-glycan composition analysis	63:99	related N-glycan composition analysis	63:99	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	4	1	theme	N-glycan	788:795	arg1	structure					797:805	each N-glycan structure	783:805	each N-glycan structure	783:805	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	2	gly	glycoprotein	893:904	arg1	glycoprotein					893:904	the whole glycoprotein	883:904	the whole glycoprotein	883:904	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	0	3	theme	N-glycan	71:78	arg1	analysis					92:99	related N-glycan composition analysis	63:99	related N-glycan composition analysis	63:99	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	7	4	theme	native	1345:1350	arg1	G-hPRL					1352:1357	native G-hPRL	1345:1357	native G-hPRL	1345:1357	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	2	5	contain	carrying	402:409	arg2	cDNA					420:423	the hPRL cDNA	411:423	the hPRL cDNA	411:423	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	2	5	contain	carrying	402:409	arg1	vector					395:400	the pcDNA-3.4-TOPO® vector	375:400	the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA	375:423	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	2	6	theme	kidney	323:328	arg1	cells					339:343	human embryonic kidney (HEK293) cells	307:343	human embryonic kidney (HEK293) cells	307:343	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	7	7	with	difference	1223:1232	arg1	latter					1243:1248	latter	1243:1248	latter	1243:1248	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	0	8	from	Expression	0:9	arg1	cells					53:57	HEK293 cells	46:57	HEK293 cells	46:57	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	2	9	from	synthesis	286:294	arg1	cells					339:343	human embryonic kidney (HEK293) cells	307:343	human embryonic kidney (HEK293) cells	307:343	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	1	10	theme	immune	236:241	arg1	regulation					243:252	immune regulation	236:252	immune regulation	236:252	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	4	11	dep	mass	853:856	arg1	ii					832:833	ii	832:833	ii	832:833	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	11	dep	mass	853:856	arg1	fraction					937:944	fraction	937:944	fraction	937:944	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	12	theme	in	1365:1366	arg1	bioactivity					1375:1385	The "in vitro" bioactivity	1360:1385	The "in vitro" bioactivity of HEK-G-hPRL	1360:1399	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	3	13	theme	human	519:523	arg1	prolactin					525:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	2	14	theme	present	259:265	arg1	work					267:270	The present work	255:270	The present work	255:270	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	7	15	located	observed	1333:1340	arg2	that					1328:1331	that	1328:1331	that	1328:1331	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	7	15	located	observed	1333:1340	arg1	G-hPRL					1352:1357	native G-hPRL	1345:1357	native G-hPRL	1345:1357	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	7	16	dep	lower	1296:1300	arg1	sialylation					1261:1271	sialylation	1261:1271	sialylation	1261:1271	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	3	17	theme	glycosylated	466:477	arg1	prolactin					525:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	3	18	dep	glycosylated	466:477	arg1	G-hPRL					480:485	G-hPRL	480:485	G-hPRL	480:485	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	6	19	link	CHO-derived	1194:1204	arg1	G-hPRL					1206:1211	CHO-derived G-hPRL	1194:1211	CHO-derived G-hPRL	1194:1211	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	1	20	theme	pituitary	147:155	arg1	gland					157:161	the pituitary gland	143:161	the pituitary gland	143:161	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	4	21	theme	glycoprotein	893:904	arg1	mass					932:935	relative carbohydrate mass	910:935	relative carbohydrate mass	910:935	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	21	theme	glycoprotein	893:904	arg1	mass					853:856	average N-glycan mass	836:856	average N-glycan mass	836:856	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	21	theme	glycoprotein	893:904	arg1	mass					875:878	(iii) molecular mass	859:878	(iii) molecular mass of the whole glycoprotein	859:904	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	22	theme	"	1373:1373	arg1	bioactivity					1375:1385	The "in vitro" bioactivity	1360:1385	The "in vitro" bioactivity of HEK-G-hPRL	1360:1399	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	4	23	theme	relative	910:917	arg1	mass					932:935	relative carbohydrate mass	910:935	relative carbohydrate mass	910:935	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	24	theme	carbohydrate	919:930	arg1	mass					932:935	relative carbohydrate mass	910:935	relative carbohydrate mass	910:935	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	25	theme	molecular	865:873	arg1	mass					875:878	(iii) molecular mass	859:878	(iii) molecular mass of the whole glycoprotein	859:904	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	26	theme	wide	658:661	arg1	range					663:667	the wide range	654:667	the wide range of 5-30% reported for pituitary-derived hPRL	654:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	2	27	theme	hPRL	415:418	arg1	cDNA					420:423	the hPRL cDNA	411:423	the hPRL cDNA	411:423	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	6	28	theme	HEK-derived	1076:1086	arg1	G-hPRL					1088:1093	HEK-derived G-hPRL	1076:1093	HEK-derived G-hPRL	1076:1093	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	3	29	theme	CHO-derived	610:620	arg1	hPRL					622:625	CHO-derived hPRL	610:625	CHO-derived hPRL (~ 10%)	610:633	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	3	29	theme	CHO-derived	610:620	arg1	%					632:632	~ 10%	628:632	~ 10%	628:632	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	4	30	dep	fraction	957:964	arg1	iv					948:949	iv	948:949	iv	948:949	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	31	dep	mass	875:878	arg1	iii					860:862	iii	860:862	iii	860:862	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	7	32	theme	main	1218:1221	arg1	due					1254:1256	due	1254:1256	due	1254:1256	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	7	32	theme	main	1218:1221	arg1	difference					1223:1232	The main difference	1214:1232	The main difference with the latter	1214:1248	The main difference with the latter was due to sialylation, which was ~ sevenfold lower, but slightly higher than that observed in native G-hPRL.					
31468229	1	33	theme	innumerable	168:178	arg1	reproduction					210:221	reproduction	210:221	reproduction	210:221	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	1	33	theme	innumerable	168:178	arg1	lactation					199:207	lactation	199:207	lactation	199:207	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	1	33	theme	innumerable	168:178	arg1	osmotic					224:230	osmotic	224:230	osmotic	224:230	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	1	33	theme	innumerable	168:178	arg1	functions					180:188	innumerable functions	168:188	innumerable functions	168:188	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	1	33	theme	innumerable	168:178	arg1	regulation					243:252	immune regulation	236:252	immune regulation	236:252	Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation.					
31468229	0	34	theme	human	27:31	arg1	prolactin					33:41	glycosylated human prolactin	14:41	glycosylated human prolactin	14:41	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	3	35	theme	%	676:676	arg1	range					663:667	the wide range	654:667	the wide range of 5-30% reported for pituitary-derived hPRL	654:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	6	36	theme	CHO-derived	1194:1204	arg1	G-hPRL					1206:1211	CHO-derived G-hPRL	1194:1211	CHO-derived G-hPRL	1194:1211	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	4	37	theme	monosaccharide	974:987	arg1	fraction					957:964	(iv) mass fraction	947:964	(iv) mass fraction of each monosaccharide	947:987	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	38	theme	concentration	428:440	arg1	of ~ 20 mg/L					442:453	A concentration of ~ 20 mg/L	426:453	A concentration of ~ 20 mg/L	426:453	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	0	39	theme	glycosylated	14:25	arg1	prolactin					33:41	glycosylated human prolactin	14:41	glycosylated human prolactin	14:41	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	2	40	theme	pcDNA-3.4-TOPO®	379:393	arg1	vector					395:400	the pcDNA-3.4-TOPO® vector	375:400	the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA	375:423	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	4	41	theme	whole	887:891	arg1	glycoprotein					893:904	the whole glycoprotein	883:904	the whole glycoprotein	883:904	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	42	theme	HEK-G-hPRL	1390:1399	arg1	bioactivity					1375:1385	The "in vitro" bioactivity	1360:1385	The "in vitro" bioactivity of HEK-G-hPRL	1360:1399	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	4	43	theme	N-glycan	844:851	arg1	mass					853:856	average N-glycan mass	836:856	average N-glycan mass	836:856	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	44	theme	structures	1506:1515	arg1	number					1487:1492	the number	1483:1492	the number of N-glycan structures	1483:1515	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	4	45	dep	provided	751:758	arg1	identification					765:778	(i) identification	761:778	(i) identification of each N-glycan structure and relative intensity	761:828	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	45	dep	provided	751:758	arg1	fraction					957:964	(iv) mass fraction	947:964	(iv) mass fraction of each monosaccharide	947:987	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	45	dep	provided	751:758	arg1	mass					853:856	average N-glycan mass	836:856	average N-glycan mass	836:856	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	45	dep	provided	751:758	arg1	mass					875:878	(iii) molecular mass	859:878	(iii) molecular mass of the whole glycoprotein	859:904	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	4	45	dep	provided	751:758	arg1	mass					932:935	relative carbohydrate mass	910:935	relative carbohydrate mass	910:935	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	0	46	theme	prolactin	33:41	arg1	Expression					0:9	Expression	0:9	Expression of glycosylated human prolactin in HEK293 cells	0:57	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	0	46	theme	prolactin	33:41	arg1	analysis					92:99	related N-glycan composition analysis	63:99	related N-glycan composition analysis	63:99	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	3	47	theme	pituitary-derived	691:707	arg1	hPRL					709:712	pituitary-derived hPRL	691:712	pituitary-derived hPRL	691:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	4	48	theme	average	836:842	arg1	mass					853:856	average N-glycan mass	836:856	average N-glycan mass	836:856	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	6	49	theme	whole	1064:1068	arg1	25,123 Da					1129:1137	25,123 Da	1129:1137	25,123 Da	1129:1137	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	6	49	theme	whole	1064:1068	arg1	MM					1070:1071	The whole MM	1060:1071	The whole MM	1060:1071	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	4	50	theme	G-hPRL	744:749	arg1	analysis					732:739	N-Glycoprofiling analysis	715:739	N-Glycoprofiling analysis of G-hPRL	715:749	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	5	51	link	CHO-derived	1040:1050	arg1	G-hPRL					1052:1057	pituitary- and CHO-derived G-hPRL	1025:1057	pituitary- and CHO-derived G-hPRL	1025:1057	The data obtained were compared to pituitary- and CHO-derived G-hPRL.					
31468229	5	52	theme	pituitary-	1025:1034	arg1	G-hPRL					1052:1057	pituitary- and CHO-derived G-hPRL	1025:1057	pituitary- and CHO-derived G-hPRL	1025:1057	The data obtained were compared to pituitary- and CHO-derived G-hPRL.					
31468229	4	53	theme	intensity	820:828	arg1	identification					765:778	(i) identification	761:778	(i) identification of each N-glycan structure and relative intensity	761:828	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	54	dep	lower	1416:1420	arg1	bioactivity					1375:1385	The "in vitro" bioactivity	1360:1385	The "in vitro" bioactivity of HEK-G-hPRL	1360:1399	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	0	55	gly	glycosylated	14:25	arg1	prolactin					33:41	glycosylated human prolactin	14:41	glycosylated human prolactin	14:41	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	2	56	theme	embryonic	313:321	arg1	HEK293					331:336	HEK293	331:336	HEK293	331:336	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	2	56	theme	embryonic	313:321	arg1	kidney					323:328	human embryonic kidney	307:328	human embryonic kidney (HEK293) cells	307:343	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	0	57	theme	HEK293	46:51	arg1	cells					53:57	HEK293 cells	46:57	HEK293 cells	46:57	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	5	58	theme	CHO-derived	1040:1050	arg1	G-hPRL					1052:1057	pituitary- and CHO-derived G-hPRL	1025:1057	pituitary- and CHO-derived G-hPRL	1025:1057	The data obtained were compared to pituitary- and CHO-derived G-hPRL.					
31468229	4	59	theme	relative	811:818	arg1	intensity					820:828	relative intensity	811:828	relative intensity	811:828	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	60	link	pituitary-derived	691:707	arg1	hPRL					709:712	pituitary-derived hPRL	691:712	pituitary-derived hPRL	691:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	2	61	theme	human	307:311	arg1	HEK293					331:336	HEK293	331:336	HEK293	331:336	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	2	61	theme	human	307:311	arg1	kidney					323:328	human embryonic kidney	307:328	human embryonic kidney (HEK293) cells	307:343	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	8	62	dep	in	1365:1366	arg1	vitro					1368:1372	vitro	1368:1372	vitro	1368:1372	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	4	63	theme	N-Glycoprofiling	715:730	arg1	analysis					732:739	N-Glycoprofiling analysis	715:739	N-Glycoprofiling analysis of G-hPRL	715:749	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	64	link	CHO-derived	610:620	arg1	hPRL					622:625	CHO-derived hPRL	610:625	CHO-derived hPRL (~ 10%)	610:633	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	3	64	link	CHO-derived	610:620	arg1	%					632:632	~ 10%	628:632	~ 10%	628:632	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	6	65	link	HEK-derived	1076:1086	arg1	G-hPRL					1088:1093	HEK-derived G-hPRL	1076:1093	HEK-derived G-hPRL	1076:1093	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	6	66	theme	G-hPRL	1088:1093	arg1	25,123 Da					1129:1137	25,123 Da	1129:1137	25,123 Da	1129:1137	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	6	66	theme	G-hPRL	1088:1093	arg1	MM					1070:1071	The whole MM	1060:1071	The whole MM	1060:1071	The whole MM of HEK-derived G-hPRL, determined via MALDI-TOF-MS, was 25,123 Da, which is 0.88% higher than pit- and 0.61% higher than CHO-derived G-hPRL.					
31468229	2	67	theme	hPRL	299:302	arg1	synthesis					286:294	the synthesis	282:294	the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA	282:423	The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO® vector carrying the hPRL cDNA.					
31468229	4	68	theme	structure	797:805	arg1	identification					765:778	(i) identification	761:778	(i) identification of each N-glycan structure and relative intensity	761:828	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	8	69	theme	native	1435:1440	arg1	G-hPRL					1442:1447	native G-hPRL	1435:1447	native G-hPRL	1435:1447	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
31468229	0	70	theme	related	63:69	arg1	analysis					92:99	related N-glycan composition analysis	63:99	related N-glycan composition analysis	63:99	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	0	71	from	analysis	92:99	arg1	cells					53:57	HEK293 cells	46:57	HEK293 cells	46:57	Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis.					
31468229	3	72	theme	G-hPRL	564:569	arg1	%					559:559	with ~ 19%	550:559	with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL	550:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	3	72	theme	G-hPRL	564:569	arg1	G-hPRL					564:569	G-hPRL	564:569	G-hPRL	564:569	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	4	73	theme	mass	952:955	arg1	fraction					957:964	(iv) mass fraction	947:964	(iv) mass fraction of each monosaccharide	947:987	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	74	dep	that	593:596	arg1	observed					598:605	observed	598:605	observed in CHO-derived hPRL (~ 10%)	598:633	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	3	74	dep	that	593:596	arg1	falling					639:645	falling	639:645	falling within the wide range of 5-30% reported for pituitary-derived hPRL	639:712	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	4	75	dep	identification	765:778	arg1	i					762:762	i	762:762	i	762:762	N-Glycoprofiling analysis of G-hPRL provided: (i) identification of each N-glycan structure and relative intensity; (ii) average N-glycan mass; (iii) molecular mass of the whole glycoprotein and relative carbohydrate mass fraction; (iv) mass fraction of each monosaccharide.					
31468229	3	76	theme	non-glycosylated	492:507	arg1	prolactin					525:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin	466:533	A concentration of ~ 20 mg/L, including glycosylated (G-hPRL) and non-glycosylated (NG-hPRL) human prolactin, was obtained, with ~ 19% of G-hPRL, which is higher than that observed in CHO-derived hPRL (~ 10%) and falling within the wide range of 5-30% reported for pituitary-derived hPRL.					
31468229	8	77	theme	N-glycan	1497:1504	arg1	structures					1506:1515	N-glycan structures	1497:1515	N-glycan structures	1497:1515	The "in vitro" bioactivity of HEK-G-hPRL was ~ fourfold lower than that of native G-hPRL, with which it had in common also the number of N-glycan structures.					
29685348	6	0	theme	rDApu	899:903	arg1	activity					887:894	The highest activity	875:894	The highest activity of rDApu	875:903	The highest activity of rDApu was measured at pH 5.0 and 95 °C.					
29685348	2	1	theme	utilization	384:394	arg1	cluster					401:407	a putative carbohydrate utilization gene cluster	360:407	a putative carbohydrate utilization gene cluster	360:407	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	0	2	theme	branched	81:88	arg1	cyclodextrin					90:101	highly branched cyclodextrin	74:101	highly branched cyclodextrin	74:101	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	1	3	theme	hyperthermophilic	189:205	arg1	amylolyticus					156:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	1	3	theme	hyperthermophilic	189:205	arg1	crenarchaeon					207:218	an anaerobic and hyperthermophilic crenarchaeon	172:218	an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources	172:271	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	8	4	theme	MALDI-TOF/TOF	1132:1144	arg1	MS					1146:1147	MALDI-TOF/TOF MS	1132:1147	MALDI-TOF/TOF MS	1132:1147	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	2	5	theme	carbohydrate	371:382	arg1	cluster					401:407	a putative carbohydrate utilization gene cluster	360:407	a putative carbohydrate utilization gene cluster	360:407	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	6	6	theme	highest	879:885	arg1	activity					887:894	The highest activity	875:894	The highest activity of rDApu	875:903	The highest activity of rDApu was measured at pH 5.0 and 95 °C.					
29685348	3	7	theme	71 kDa	548:553	arg1	mass					540:543	a molecular mass	528:543	a molecular mass of 71 kDa	528:553	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	2	8	theme	family	321:326	arg1	enzymes					342:348	family 57 amylolytic enzymes	321:348	a glycoside hydrolase family 57 amylolytic enzymes (DApu)	299:355	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	0	9	from	9188	60:63	arg1	amylopullulanase					5:20	GH57 amylopullulanase	0:20	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188	0:63	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	3	10	theme	molecular	530:538	arg1	mass					540:543	a molecular mass	528:543	a molecular mass of 71 kDa	528:553	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	0	11	theme	transglycosylation	111:128	arg1	activity					130:137	its transglycosylation activity	107:137	its transglycosylation activity	107:137	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	7	12	theme	maltose	1047:1053	arg1	unit					1055:1058	a maltose unit	1045:1058	a maltose unit to 6-O-α-maltosyl-β-CD	1045:1081	The half-life of rDApu was 12.7 h at 95 °C and 27 min at 98 °C. Interestingly, rDApu was able to transfer a maltose unit to 6-O-α-maltosyl-β-CD via transglycosylation.					
29685348	2	13	theme	glycoside	301:309	arg1	hydrolase					311:319	a glycoside hydrolase family 57 amylolytic enzymes	299:348	a glycoside hydrolase family 57 amylolytic enzymes (DApu)	299:355	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	2	13	theme	glycoside	301:309	arg1	DApu					351:354	DApu	351:354	DApu	351:354	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	3	14	theme	open	460:463	arg1	frame					473:477	an open reading frame	457:477	an open reading frame of 1,878 bp	457:489	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	8	15	theme	magnetic	1161:1168	arg1	resonance					1170:1178	nuclear magnetic resonance	1153:1178	nuclear magnetic resonance	1153:1178	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	8	16	theme	Structure	1107:1115	arg1	analysis					1117:1124	Structure analysis	1107:1124	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance	1107:1178	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	0	17	theme	GH57	0:3	arg1	amylopullulanase					5:20	GH57 amylopullulanase	0:20	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188	0:63	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	4	18	theme	Recombinant	556:566	arg1	rDApu					574:578	rDApu	574:578	rDApu	574:578	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	4	18	theme	Recombinant	556:566	arg1	DApu					568:571	Recombinant DApu	556:571	Recombinant DApu (rDApu)	556:579	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	0	19	theme	Desulfurococcus	27:41	arg1	9188					60:63	Desulfurococcus amylolyticus JCM 9188	27:63	Desulfurococcus amylolyticus JCM 9188	27:63	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	4	20	from	amylopectin	735:745	arg1	maltose					703:709	maltose	703:709	maltose	703:709	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	4	20	from	amylopectin	735:745	arg1	glucose					691:697	glucose	691:697	glucose	691:697	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	1	21	theme	various	233:239	arg1	carbohydrates					241:253	various carbohydrates	233:253	various carbohydrates	233:253	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	1	21	theme	various	233:239	arg1	sources					265:271	energy sources	258:271	energy sources	258:271	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	2	22	theme	amylolytic	331:340	arg1	enzymes					342:348	family 57 amylolytic enzymes	321:348	a glycoside hydrolase family 57 amylolytic enzymes (DApu)	299:355	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	3	23	theme	1,878 bp	482:489	arg1	frame					473:477	an open reading frame	457:477	an open reading frame of 1,878 bp	457:489	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	0	24	theme	JCM	56:58	arg1	9188					60:63	Desulfurococcus amylolyticus JCM 9188	27:63	Desulfurococcus amylolyticus JCM 9188	27:63	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	3	25	theme	reading	465:471	arg1	frame					473:477	an open reading frame	457:477	an open reading frame of 1,878 bp	457:489	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	0	26	theme	amylolyticus	43:54	arg1	9188					60:63	Desulfurococcus amylolyticus JCM 9188	27:63	Desulfurococcus amylolyticus JCM 9188	27:63	GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.					
29685348	2	27	dep	D.	426:427	arg1	amylolyticus					429:440	D. amylolyticus	426:440	D. amylolyticus	426:440	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	2	28	theme	D.	426:427	arg1	genome					416:421	the genome	412:421	the genome of D. amylolyticus	412:440	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	8	29	theme	transglycosylated	1202:1218	arg1	61					1234:1235	61	1234:1235	61	1234:1235	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	8	29	theme	transglycosylated	1202:1218	arg1	products					1220:1227	the new transglycosylated products	1194:1227	the new transglycosylated products	1194:1227	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	3	30	theme	amino	511:515	arg1	acids					517:521	626 amino acids	507:521	626 amino acids	507:521	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	5	31	with	reaction	855:862	arg1	γ-CD					869:872	γ-CD	869:872	γ-CD	869:872	Although rDApu showed no activity toward α-cyclodextrin (CD) and β-CD, maltooctaose (G8) was detected from reaction with γ-CD.					
29685348	2	32	theme	putative	362:369	arg1	cluster					401:407	a putative carbohydrate utilization gene cluster	360:407	a putative carbohydrate utilization gene cluster	360:407	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	8	33	theme	new	1198:1200	arg1	61					1234:1235	61	1234:1235	61	1234:1235	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	8	33	theme	new	1198:1200	arg1	products					1220:1227	the new transglycosylated products	1194:1227	the new transglycosylated products	1194:1227	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	3	34	contain	has	453:455	arg1	gene					448:451	This gene	443:451	This gene	443:451	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	3	34	contain	has	453:455	arg2	frame					473:477	an open reading frame	457:477	an open reading frame of 1,878 bp	457:489	This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa.					
29685348	1	35	theme	Desulfurococcus	140:154	arg1	amylolyticus					156:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	1	35	theme	Desulfurococcus	140:154	arg1	crenarchaeon					207:218	an anaerobic and hyperthermophilic crenarchaeon	172:218	an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources	172:271	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	8	36	gly	transglycosylated	1202:1218	arg1	61					1234:1235	61	1234:1235	61	1234:1235	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	8	36	gly	transglycosylated	1202:1218	arg1	products					1220:1227	the new transglycosylated products	1194:1227	the new transglycosylated products	1194:1227	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	4	37	theme	soluble	716:722	arg1	starch					724:729	soluble starch	716:729	soluble starch	716:729	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	4	38	from	starch	724:729	arg1	maltose					703:709	maltose	703:709	maltose	703:709	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	4	38	from	starch	724:729	arg1	glucose					691:697	glucose	691:697	glucose	691:697	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	8	39	theme	nuclear	1153:1159	arg1	resonance					1170:1178	nuclear magnetic resonance	1153:1178	nuclear magnetic resonance	1153:1178	Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD.					
29685348	7	40	theme	rDApu	956:960	arg1	half-life					943:951	The half-life	939:951	The half-life of rDApu	939:960	The half-life of rDApu was 12.7 h at 95 °C and 27 min at 98 °C. Interestingly, rDApu was able to transfer a maltose unit to 6-O-α-maltosyl-β-CD via transglycosylation.					
29685348	2	41	theme	gene	396:399	arg1	cluster					401:407	a putative carbohydrate utilization gene cluster	360:407	a putative carbohydrate utilization gene cluster	360:407	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	1	42	theme	energy	258:263	arg1	carbohydrates					241:253	various carbohydrates	233:253	various carbohydrates	233:253	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	1	42	theme	energy	258:263	arg1	sources					265:271	energy sources	258:271	energy sources	258:271	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	4	43	theme	α-1,6-glycosidic	640:655	arg1	linkages					657:664	α-1,6-glycosidic linkages	640:664	α-1,6-glycosidic linkages	640:664	Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin.					
29685348	2	44	dep	hydrolase	311:319	arg1	enzymes					342:348	family 57 amylolytic enzymes	321:348	a glycoside hydrolase family 57 amylolytic enzymes (DApu)	299:355	We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus.					
29685348	1	45	theme	anaerobic	175:183	arg1	amylolyticus					156:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus	140:167	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
29685348	1	45	theme	anaerobic	175:183	arg1	crenarchaeon					207:218	an anaerobic and hyperthermophilic crenarchaeon	172:218	an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources	172:271	Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources.					
30293150	5	0	theme	AglW	796:799	arg1	activity					784:791	the 4-epimerase activity	768:791	the 4-epimerase activity of AglW	768:799	The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties.					
30293150	0	1	from	characterization	19:34	arg1	maripaludis					92:102	the archaeon Methanococcus maripaludis	65:102	the archaeon Methanococcus maripaludis	65:102	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	7	2	theme	broad	1137:1141	arg1	specificity					1143:1153	a broad specificity	1135:1153	a broad specificity towards UDP-sugars	1135:1172	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	5	3	theme	work	730:733	arg1	goal					718:721	The goal	714:721	The goal of our work	714:733	The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties.					
30293150	2	4	from	archaella	379:387	arg1	surface					401:407	its cell surface	392:407	its cell surface	392:407	Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface.					
30293150	1	5	theme	harsh	182:186	arg1	conditions					188:197	harsh conditions	182:197	harsh conditions	182:197	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	0	6	from	Identification	0:13	arg1	maripaludis					92:102	the archaeon Methanococcus maripaludis	65:102	the archaeon Methanococcus maripaludis	65:102	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	2	7	theme	cell	396:399	arg1	surface					401:407	its cell surface	392:407	its cell surface	392:407	Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface.					
30293150	3	8	theme	archaella	590:598	arg1	function					578:585	function	578:585	function	578:585	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	8	theme	archaella	590:598	arg1	assembly					565:572	proper assembly	558:572	proper assembly	558:572	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	4	9	theme	4-epimerase	638:648	arg1	AglW					650:653	the putative 4-epimerase AglW	625:653	the putative 4-epimerase AglW	625:653	The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide.					
30293150	7	10	theme	4-epimerase	1243:1253	arg1	function					1255:1262	the 4-epimerase function	1239:1262	the 4-epimerase function of AglW	1239:1270	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	2	11	theme	methane-producing	324:340	arg1	archaeon					342:349	a methane-producing archaeon	322:349	a methane-producing archaeon	322:349	Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface.					
30293150	2	11	theme	methane-producing	324:340	arg1	maripaludis					309:319	Methanococcus maripaludis	295:319	Methanococcus maripaludis	295:319	Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface.					
30293150	4	12	theme	putative	629:636	arg1	AglW					650:653	the putative 4-epimerase AglW	625:653	the putative 4-epimerase AglW	625:653	The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide.					
30293150	8	13	theme	novel	1383:1387	arg1	4-epimerases					1389:1400	novel 4-epimerases	1383:1400	novel 4-epimerases	1383:1400	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	4	14	theme	tetrasaccharide	697:711	arg1	synthesis					680:688	the synthesis	676:688	the synthesis of the tetrasaccharide	676:711	The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide.					
30293150	8	15	theme	specificity	1360:1370	arg1	studies					1372:1378	specificity studies	1360:1378	specificity studies	1360:1378	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	5	16	theme	4-epimerase	772:782	arg1	activity					784:791	the 4-epimerase activity	768:791	the 4-epimerase activity of AglW	768:799	The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties.					
30293150	7	17	theme	conserved	1199:1207	arg1	sequon					1215:1220	a conserved YxxxK sequon	1197:1220	a conserved YxxxK sequon	1197:1220	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	3	18	theme	archaellum	461:470	arg1	proteins					431:438	The major structural proteins	410:438	The major structural proteins (archaellins) of the archaellum	410:470	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	18	theme	archaellum	461:470	arg1	glycoproteins					476:488	glycoproteins	476:488	glycoproteins	476:488	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	18	theme	archaellum	461:470	arg1	archaellins					441:451	archaellins	441:451	archaellins	441:451	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	8	19	theme	glycosyltransferase-coupled	1277:1303	arg1	assays					1305:1310	The glycosyltransferase-coupled assays	1273:1310	The glycosyltransferase-coupled assays	1273:1310	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	8	19	theme	glycosyltransferase-coupled	1277:1303	arg1	useful					1326:1331	useful	1326:1331	useful	1326:1331	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	4	20	theme	aglW	605:608	arg1	gene					610:613	The aglW gene	601:613	The aglW gene	601:613	The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide.					
30293150	1	21	theme	important	208:216	arg1	roles					218:222	important roles	208:222	important roles	208:222	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	3	22	theme	major	414:418	arg1	proteins					431:438	The major structural proteins	410:438	The major structural proteins (archaellins) of the archaellum	410:470	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	22	theme	major	414:418	arg1	glycoproteins					476:488	glycoproteins	476:488	glycoproteins	476:488	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	22	theme	major	414:418	arg1	archaellins					441:451	archaellins	441:451	archaellins	441:451	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	8	23	dep	identification	1341:1354	arg1	the					1337:1339	the	1337:1339	the	1337:1339	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	3	24	dep	assembly	565:572	arg1	the					554:556	the	554:556	the	554:556	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	25	theme	N-linked	505:512	arg1	tetrasaccharides					514:529	N-linked tetrasaccharides	505:529	N-linked tetrasaccharides that are essential for the proper assembly and function of archaella	505:598	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	26	theme	structural	420:429	arg1	proteins					431:438	The major structural proteins	410:438	The major structural proteins (archaellins) of the archaellum	410:470	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	26	theme	structural	420:429	arg1	glycoproteins					476:488	glycoproteins	476:488	glycoproteins	476:488	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	26	theme	structural	420:429	arg1	archaellins					441:451	archaellins	441:451	archaellins	441:451	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	1	27	with	cycles	242:247	arg1	applications					264:275	potential applications	254:275	potential applications in biotechnology	254:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	0	28	from	maripaludis	92:102	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	0	28	from	maripaludis	92:102	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	0	28	from	maripaludis	92:102	arg1	AglW					55:58	the 4-epimerase AglW	39:58	the 4-epimerase AglW from the archaeon Methanococcus maripaludis	39:102	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	1	29	theme	ubiquitous	117:126	arg1	Archaea					105:111	Archaea	105:111	Archaea	105:111	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	1	29	theme	ubiquitous	117:126	arg1	microorganisms					140:153	ubiquitous single-cell microorganisms	117:153	ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology	117:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	4	30	theme	key	664:666	arg1	role					668:671	a key role	662:671	a key role	662:671	The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide.					
30293150	0	31	theme	4-epimerase	43:53	arg1	AglW					55:58	the 4-epimerase AglW	39:58	the 4-epimerase AglW from the archaeon Methanococcus maripaludis	39:102	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	6	32	contain	carried	882:888	arg2	assays					894:899	assays	894:899	assays	894:899	We carried out assays using UDP-Galactose, UDP-Glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and N-acetylglucosamine/N-acetylgalactosamine-diphosphate - lipid as substrates, coupled with specific glycosyltransferases.					
30293150	6	32	contain	carried	882:888	arg1	We					879:880	We	879:880	We	879:880	We carried out assays using UDP-Galactose, UDP-Glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and N-acetylglucosamine/N-acetylgalactosamine-diphosphate - lipid as substrates, coupled with specific glycosyltransferases.					
30293150	7	33	contain	has	1131:1133	arg2	specificity					1143:1153	a broad specificity	1135:1153	a broad specificity towards UDP-sugars	1135:1172	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	7	33	contain	has	1131:1133	arg1	AglW					1126:1129	AglW	1126:1129	AglW	1126:1129	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	1	34	theme	biogeochemical	227:240	arg1	cycles					242:247	biogeochemical cycles	227:247	biogeochemical cycles with potential applications in biotechnology	227:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	1	35	from	applications	264:275	arg1	biotechnology					280:292	biotechnology	280:292	biotechnology	280:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	1	36	theme	single-cell	128:138	arg1	Archaea					105:111	Archaea	105:111	Archaea	105:111	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	1	36	theme	single-cell	128:138	arg1	microorganisms					140:153	ubiquitous single-cell microorganisms	117:153	ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology	117:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	0	37	theme	AglW	55:58	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	0	37	theme	AglW	55:58	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.					
30293150	3	38	theme	proper	558:563	arg1	assembly					565:572	proper assembly	558:572	proper assembly	558:572	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	39	link	N-linked	505:512	arg1	tetrasaccharides					514:529	N-linked tetrasaccharides	505:529	N-linked tetrasaccharides that are essential for the proper assembly and function of archaella	505:598	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	40	gly	glycoproteins	476:488	arg1	glycoproteins					476:488	glycoproteins	476:488	glycoproteins	476:488	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	3	40	gly	glycoproteins	476:488	arg1	proteins					431:438	The major structural proteins	410:438	The major structural proteins (archaellins) of the archaellum	410:470	The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.					
30293150	7	41	theme	YxxxK	1209:1213	arg1	sequon					1215:1220	a conserved YxxxK sequon	1197:1220	a conserved YxxxK sequon	1197:1220	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
30293150	2	42	theme	multiple	370:377	arg1	archaella					379:387	multiple archaella	370:387	multiple archaella on its cell surface	370:407	Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface.					
30293150	1	43	theme	potential	254:262	arg1	applications					264:275	potential applications	254:275	potential applications in biotechnology	254:292	Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.					
30293150	8	44	theme	4-epimerases	1389:1400	arg1	studies					1372:1378	specificity studies	1360:1378	specificity studies	1360:1378	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	8	44	theme	4-epimerases	1389:1400	arg1	identification					1341:1354	identification	1341:1354	identification	1341:1354	The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.					
30293150	6	45	theme	specific	1080:1087	arg1	glycosyltransferases					1089:1108	specific glycosyltransferases	1080:1108	specific glycosyltransferases	1080:1108	We carried out assays using UDP-Galactose, UDP-Glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and N-acetylglucosamine/N-acetylgalactosamine-diphosphate - lipid as substrates, coupled with specific glycosyltransferases.					
30293150	5	46	theme	substrate	841:849	arg1	specificity					851:861	its substrate specificity	837:861	its substrate specificity	837:861	The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties.					
30293150	7	47	theme	AglW	1267:1270	arg1	function					1255:1262	the 4-epimerase function	1239:1262	the 4-epimerase function of AglW	1239:1270	We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW.					
31869330	4	0	theme	related	895:901	arg1	polysaccharea					914:926	N. polysaccharea	911:926	N. polysaccharea	911:926	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	0	theme	related	895:901	arg1	lactamica					935:943	N. lactamica	932:943	N. lactamica	932:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	0	theme	related	895:901	arg1	species					903:909	the closely related species	883:909	the closely related species N. polysaccharea and N. lactamica	883:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	3	1	theme	genome	702:707	arg1	genes					688:692	those pgl genes	678:692	those pgl genes	678:692	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	3	1	theme	genome	702:707	arg1	constituents					709:720	core genome constituents	697:720	core genome constituents of the genus	697:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	4	2	theme	N.	911:912	arg1	polysaccharea					914:926	N. polysaccharea	911:926	N. polysaccharea	911:926	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	2	theme	N.	911:912	arg1	species					903:909	the closely related species	883:909	the closely related species N. polysaccharea and N. lactamica	883:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	3	3	theme	genus	729:733	arg1	genes					688:692	those pgl genes	678:692	those pgl genes	678:692	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	3	3	theme	genus	729:733	arg1	constituents					709:720	core genome constituents	697:720	core genome constituents of the genus	697:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	1	4	theme	interstrain	179:189	arg1	diversity					198:206	interstrain glycan diversity	179:206	interstrain glycan diversity	179:206	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	7	5	theme	epistasis	1501:1509	arg1	processes					1474:1482	processes	1474:1482	processes of compositional epistasis leading to gene decay	1474:1531	We suggest that the pseudogenization events are driven by processes of compositional epistasis leading to gene decay.					
31869330	6	6	dep	identification	1216:1229	arg1	Critical					1181:1188	Critical	1181:1188	Critical	1181:1188	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	3	7	theme	subset	585:590	arg1	conservation					567:578	the conservation	563:578	the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus	563:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	9	8	theme	glycosylation	1823:1835	arg1	systems					1837:1843	protein glycosylation systems	1815:1843	protein glycosylation systems	1815:1843	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	1	9	theme	O-linked	230:237	arg1	pgl					262:264	pgl	262:264	pgl	262:264	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	9	theme	O-linked	230:237	arg1	glycosylation					247:259	O-linked protein glycosylation	230:259	their O-linked protein glycosylation (pgl) systems	224:273	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	3	10	theme	genes	595:599	arg1	subset					585:590	a subset	583:590	a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus	583:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	7	11	theme	pseudogenization	1436:1451	arg1	events					1453:1458	the pseudogenization events	1432:1458	the pseudogenization events	1432:1458	We suggest that the pseudogenization events are driven by processes of compositional epistasis leading to gene decay.					
31869330	6	12	theme	gene	1295:1298	arg1	identification					1216:1229	the identification	1212:1229	the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis	1212:1413	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	6	13	theme	N.	1380:1381	arg1	gonorrhoeae					1383:1393	N. gonorrhoeae	1380:1393	N. gonorrhoeae	1380:1393	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	5	14	theme	pathway	1097:1103	arg1	components					1068:1077	pgl gene components	1059:1077	pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1059:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	3	15	theme	glycosylation	641:653	arg1	glycan					655:660	O-linked protein glycosylation glycan	624:660	O-linked protein glycosylation glycan	624:660	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	4	16	from	repertoires	832:842	arg1	species					903:909	the closely related species	883:909	the closely related species N. polysaccharea and N. lactamica	883:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	16	from	repertoires	832:842	arg1	polysaccharea					914:926	N. polysaccharea	911:926	N. polysaccharea	911:926	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	16	from	repertoires	832:842	arg1	lactamica					935:943	N. lactamica	932:943	N. lactamica	932:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	16	from	repertoires	832:842	arg1	gonorrhoeae					850:860	N. gonorrhoeae	847:860	N. gonorrhoeae	847:860	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	16	from	repertoires	832:842	arg1	meningitidis					866:877	N. meningitidis	863:877	N. meningitidis	863:877	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	1	17	theme	N.	137:138	arg1	meningitidis					140:151	N. meningitidis	137:151	N. meningitidis	137:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	17	theme	N.	137:138	arg1	pathogens					108:116	The human pathogens	98:116	The human pathogens N. gonorrhoeae and N. meningitidis	98:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	4	18	theme	pathway	1016:1022	arg1	replacement					968:978	the functional replacement	953:978	the functional replacement of a progenitor glycan biosynthetic pathway	953:1022	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	8	19	theme	inter-species	1578:1590	arg1	recombination					1592:1604	inter-species recombination	1578:1604	inter-species recombination	1578:1604	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	6	20	theme	unrecognized	1262:1273	arg1	pglP					1301:1304	pglP	1301:1304	pglP	1301:1304	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	6	20	theme	unrecognized	1262:1273	arg1	gene					1295:1298	a ubiquitous but previously unrecognized glycosyltransferase gene	1234:1298	a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis	1234:1413	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	0	21	theme	bacterial	58:66	arg1	system					90:95	a bacterial protein glycosylation system	56:95	a bacterial protein glycosylation system	56:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	3	22	theme	O-linked	624:631	arg1	glycan					655:660	O-linked protein glycosylation glycan	624:660	O-linked protein glycosylation glycan	624:660	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	9	23	theme	informative	1875:1885	arg1	differences					1896:1906	phylogenetically informative, genetic differences	1858:1906	phylogenetically informative, genetic differences associated with Neisseria species	1858:1940	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	0	24	theme	glycosylation	76:88	arg1	system					90:95	a bacterial protein glycosylation system	56:95	a bacterial protein glycosylation system	56:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	8	25	theme	gene	1621:1624	arg1	status					1626:1631	pgl gene status	1617:1631	pgl gene status	1617:1631	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	9	26	dep	informative	1875:1885	arg1	genetic					1888:1894	genetic	1888:1894	genetic	1888:1894	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	2	27	theme	glycoform	512:520	arg1	diversity					522:530	glycoform diversity	512:530	glycoform diversity	512:530	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	5	28	with	coincident	1105:1114	arg1	acquisition					1125:1135	the acquisition	1121:1135	the acquisition of two exogenous glycosyltransferase genes	1121:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	5	29	theme	glycosyltransferase	1154:1172	arg1	genes					1174:1178	two exogenous glycosyltransferase genes	1140:1178	two exogenous glycosyltransferase genes	1140:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	8	30	theme	discordant	1645:1654	arg1	interactions					1664:1675	discordant genetic interactions	1645:1675	discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks	1645:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	4	31	theme	mass	756:759	arg1	phenotyping					788:798	mass spectrometric-based glycan phenotyping	756:798	mass spectrometric-based glycan phenotyping	756:798	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	0	32	theme	Genetic	0:6	arg1	determinants					8:19	Genetic determinants	0:19	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system	0:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	4	33	theme	glycan	781:786	arg1	phenotyping					788:798	mass spectrometric-based glycan phenotyping	756:798	mass spectrometric-based glycan phenotyping	756:798	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	2	34	theme	Neisseria	428:436	arg1	species					438:444	Neisseria species	428:444	Neisseria species	428:444	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	4	35	theme	N.	847:848	arg1	gonorrhoeae					850:860	N. gonorrhoeae	847:860	N. gonorrhoeae	847:860	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	2	36	theme	human-restricted	410:425	arg1	species					438:444	Neisseria species	428:444	Neisseria species	428:444	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	0	37	theme	glycan	36:41	arg1	diversity					43:51	genus-level glycan diversity	24:51	genus-level glycan diversity	24:51	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	4	38	dep	species	903:909	arg1	polysaccharea					914:926	N. polysaccharea	911:926	N. polysaccharea	911:926	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	38	dep	species	903:909	arg1	lactamica					935:943	N. lactamica	932:943	N. lactamica	932:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	38	dep	species	903:909	arg1	species					903:909	the closely related species	883:909	the closely related species N. polysaccharea and N. lactamica	883:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	5	39	theme	gene	1063:1066	arg1	components					1068:1077	pgl gene components	1059:1077	pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1059:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	2	40	theme	glycosylation	348:360	arg1	function					328:335	function	328:335	function	328:335	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	2	40	theme	glycosylation	348:360	arg1	evolution					314:322	evolution	314:322	evolution	314:322	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	3	41	theme	core	697:700	arg1	genes					688:692	those pgl genes	678:692	those pgl genes	678:692	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	3	41	theme	core	697:700	arg1	constituents					709:720	core genome constituents	697:720	core genome constituents of the genus	697:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	4	42	theme	extant	815:820	arg1	repertoires					832:842	extant glycoform repertoires	815:842	extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica	815:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	1	43	theme	protein	239:245	arg1	pgl					262:264	pgl	262:264	pgl	262:264	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	43	theme	protein	239:245	arg1	glycosylation					247:259	O-linked protein glycosylation	230:259	their O-linked protein glycosylation (pgl) systems	224:273	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	8	44	theme	pgl	1721:1723	arg1	networks					1730:1737	pgl gene networks	1721:1737	pgl gene networks	1721:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	3	45	dep	genes	595:599	arg1	established					666:676	established	666:676	established those pgl genes as core genome constituents of the genus	666:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	3	45	dep	genes	595:599	arg1	required					611:618	required	611:618	minimally required for O-linked protein glycosylation glycan	601:660	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	8	46	theme	networks	1730:1737	arg1	nature					1711:1716	the multi-locus nature	1695:1716	the multi-locus nature of pgl gene networks	1695:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	6	47	theme	independent	1348:1358	arg1	pseudogenization					1360:1375	parallel but independent pseudogenization	1335:1375	parallel but independent pseudogenization	1335:1375	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	9	48	theme	novel	1777:1781	arg1	perspective					1783:1793	a novel perspective	1775:1793	a novel perspective on the evolution of protein glycosylation systems	1775:1843	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	6	49	theme	ubiquitous	1236:1245	arg1	pglP					1301:1304	pglP	1301:1304	pglP	1301:1304	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	6	49	theme	ubiquitous	1236:1245	arg1	gene					1295:1298	a ubiquitous but previously unrecognized glycosyltransferase gene	1234:1298	a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis	1234:1413	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	6	50	theme	parallel	1335:1342	arg1	pseudogenization					1360:1375	parallel but independent pseudogenization	1335:1375	parallel but independent pseudogenization	1335:1375	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	3	51	theme	Comparative	533:543	arg1	genomics					545:552	Comparative genomics	533:552	Comparative genomics	533:552	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	4	52	theme	N.	863:864	arg1	meningitidis					866:877	N. meningitidis	863:877	N. meningitidis	863:877	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	7	53	theme	gene	1522:1525	arg1	decay					1527:1531	gene decay	1522:1531	gene decay	1522:1531	We suggest that the pseudogenization events are driven by processes of compositional epistasis leading to gene decay.					
31869330	1	54	theme	glycan	191:196	arg1	diversity					198:206	interstrain glycan diversity	179:206	interstrain glycan diversity	179:206	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	9	55	from	perspective	1783:1793	arg1	evolution					1802:1810	the evolution	1798:1810	the evolution of protein glycosylation systems	1798:1843	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	9	56	theme	protein	1815:1821	arg1	systems					1837:1843	protein glycosylation systems	1815:1843	protein glycosylation systems	1815:1843	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	7	57	theme	compositional	1487:1499	arg1	epistasis					1501:1509	compositional epistasis	1487:1509	compositional epistasis leading to gene decay	1487:1531	We suggest that the pseudogenization events are driven by processes of compositional epistasis leading to gene decay.					
31869330	4	58	theme	functional	957:966	arg1	replacement					968:978	the functional replacement	953:978	the functional replacement of a progenitor glycan biosynthetic pathway	953:1022	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	6	59	theme	N.	1399:1400	arg1	meningitidis					1402:1413	N. meningitidis	1399:1413	N. meningitidis	1399:1413	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	1	60	theme	N.	118:119	arg1	gonorrhoeae					121:131	N. gonorrhoeae	118:131	N. gonorrhoeae	118:131	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	60	theme	N.	118:119	arg1	pathogens					108:116	The human pathogens	98:116	The human pathogens N. gonorrhoeae and N. meningitidis	98:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	5	61	theme	primordial	1086:1095	arg1	pathway					1097:1103	the primordial pathway	1082:1103	the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1082:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	3	62	theme	protein	633:639	arg1	glycan					655:660	O-linked protein glycosylation glycan	624:660	O-linked protein glycosylation glycan	624:660	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	4	63	theme	N.	932:933	arg1	lactamica					935:943	N. lactamica	932:943	N. lactamica	932:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	63	theme	N.	932:933	arg1	species					903:909	the closely related species	883:909	the closely related species N. polysaccharea and N. lactamica	883:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	6	64	theme	glycosyltransferase	1275:1293	arg1	pglP					1301:1304	pglP	1301:1304	pglP	1301:1304	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	6	64	theme	glycosyltransferase	1275:1293	arg1	gene					1295:1298	a ubiquitous but previously unrecognized glycosyltransferase gene	1234:1298	a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis	1234:1413	Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis.					
31869330	2	65	dep	evolution	314:322	arg1	the					310:312	the	310:312	the	310:312	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	0	66	theme	protein	68:74	arg1	system					90:95	a bacterial protein glycosylation system	56:95	a bacterial protein glycosylation system	56:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	5	67	theme	coincident	1105:1114	arg1	pathway					1097:1103	the primordial pathway	1082:1103	the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1082:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	4	68	theme	biosynthetic	1003:1014	arg1	pathway					1016:1022	a progenitor glycan biosynthetic pathway	983:1022	a progenitor glycan biosynthetic pathway	983:1022	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	8	69	theme	pgl	1617:1619	arg1	status					1626:1631	pgl gene status	1617:1631	pgl gene status	1617:1631	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	1	70	dep	pathogens	108:116	arg1	gonorrhoeae					121:131	N. gonorrhoeae	118:131	N. gonorrhoeae	118:131	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	70	dep	pathogens	108:116	arg1	meningitidis					140:151	N. meningitidis	137:151	N. meningitidis	137:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	70	dep	pathogens	108:116	arg1	pathogens					108:116	The human pathogens	98:116	The human pathogens N. gonorrhoeae and N. meningitidis	98:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	9	71	theme	Neisseria	1924:1932	arg1	species					1934:1940	Neisseria species	1924:1940	Neisseria species	1924:1940	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	1	72	theme	robust	161:166	arg1	intra-					168:173	robust intra-	161:173	robust intra-	161:173	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	0	73	from	determinants	8:19	arg1	system					90:95	a bacterial protein glycosylation system	56:95	a bacterial protein glycosylation system	56:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	1	74	link	O-linked	230:237	arg1	pgl					262:264	pgl	262:264	pgl	262:264	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	74	link	O-linked	230:237	arg1	glycosylation					247:259	O-linked protein glycosylation	230:259	their O-linked protein glycosylation (pgl) systems	224:273	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	2	75	theme	diversity	522:530	arg1	levels					502:507	the levels	498:507	the levels of glycoform diversity	498:530	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	5	76	theme	exogenous	1144:1152	arg1	genes					1174:1178	two exogenous glycosyltransferase genes	1140:1178	two exogenous glycosyltransferase genes	1140:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	4	77	theme	progenitor	985:994	arg1	pathway					1016:1022	a progenitor glycan biosynthetic pathway	983:1022	a progenitor glycan biosynthetic pathway	983:1022	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	8	78	theme	genetic	1656:1662	arg1	interactions					1664:1675	discordant genetic interactions	1645:1675	discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks	1645:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	4	79	theme	glycan	996:1001	arg1	pathway					1016:1022	a progenitor glycan biosynthetic pathway	983:1022	a progenitor glycan biosynthetic pathway	983:1022	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	5	80	theme	genes	1174:1178	arg1	acquisition					1125:1135	the acquisition	1121:1135	the acquisition of two exogenous glycosyltransferase genes	1121:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	3	81	link	O-linked	624:631	arg1	glycan					655:660	O-linked protein glycosylation glycan	624:660	O-linked protein glycosylation glycan	624:660	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	8	82	theme	due	1677:1679	arg1	interactions					1664:1675	discordant genetic interactions	1645:1675	discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks	1645:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	0	83	theme	genus-level	24:34	arg1	diversity					43:51	genus-level glycan diversity	24:51	genus-level glycan diversity	24:51	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	1	84	theme	human	102:106	arg1	gonorrhoeae					121:131	N. gonorrhoeae	118:131	N. gonorrhoeae	118:131	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	84	theme	human	102:106	arg1	meningitidis					140:151	N. meningitidis	137:151	N. meningitidis	137:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	1	84	theme	human	102:106	arg1	pathogens					108:116	The human pathogens	98:116	The human pathogens N. gonorrhoeae and N. meningitidis	98:151	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	4	85	theme	spectrometric-based	761:779	arg1	phenotyping					788:798	mass spectrometric-based glycan phenotyping	756:798	mass spectrometric-based glycan phenotyping	756:798	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	0	86	theme	diversity	43:51	arg1	determinants					8:19	Genetic determinants	0:19	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system	0:95	Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.					
31869330	5	87	theme	pgl	1059:1061	arg1	components					1068:1077	pgl gene components	1059:1077	pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1059:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	8	88	theme	multi-locus	1699:1709	arg1	nature					1711:1716	the multi-locus nature	1695:1716	the multi-locus nature of pgl gene networks	1695:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	2	89	theme	other	404:408	arg1	species					438:444	Neisseria species	428:444	Neisseria species	428:444	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	5	90	theme	components	1068:1077	arg1	loss					1051:1054	loss	1051:1054	loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes	1051:1178	This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes.					
31869330	9	91	theme	systems	1837:1843	arg1	evolution					1802:1810	the evolution	1798:1810	the evolution of protein glycosylation systems	1798:1843	In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.					
31869330	2	92	theme	protein	340:346	arg1	glycosylation					348:360	protein glycosylation	340:360	protein glycosylation operating there	340:376	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	2	93	gly	glycosylate	456:466	arg1	proteins					468:475	proteins	468:475	proteins	468:475	In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity.					
31869330	8	94	theme	gene	1725:1728	arg1	networks					1730:1737	pgl gene networks	1721:1737	pgl gene networks	1721:1737	Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks.					
31869330	3	95	theme	pgl	684:686	arg1	genes					688:692	those pgl genes	678:692	those pgl genes	678:692	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	3	95	theme	pgl	684:686	arg1	constituents					709:720	core genome constituents	697:720	core genome constituents of the genus	697:733	Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus.					
31869330	1	96	theme	glycosylation	247:259	arg1	systems					267:273	their O-linked protein glycosylation (pgl) systems	224:273	their O-linked protein glycosylation (pgl) systems	224:273	The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems.					
31869330	4	97	with	conjunction	739:749	arg1	phenotyping					788:798	mass spectrometric-based glycan phenotyping	756:798	mass spectrometric-based glycan phenotyping	756:798	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
31869330	4	98	theme	glycoform	822:830	arg1	repertoires					832:842	extant glycoform repertoires	815:842	extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica	815:943	In conjunction with mass spectrometric-based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway.					
29529546	1	0	attach	linked	144:149	arg2	saccharides					132:142	saccharides	132:142	saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes	132:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	1	0	attach	linked	144:149	arg1	glycoproteins					154:166	glycoproteins	154:166	glycoproteins that are known to be involved in a wide range of inflammatory processes	154:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	8	1	theme	first	1672:1676	arg1	evidence					1678:1685	the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD	1668:1890	the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD	1668:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	6	2	theme	trend	1365:1369	arg1	levels					1344:1349	higher levels	1337:1349	higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients	1337:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	3	theme	N-glycan	696:703	arg1	profile					705:711	the serum N-glycan profile	686:711	the serum N-glycan profile	686:711	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	8	4	theme	immune	1857:1862	arg1	changes					1864:1870	the immune changes	1853:1870	the immune changes associated with MDD	1853:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	3	5	theme	Assisted	750:757	arg1	DSA-FACE					789:796	DSA-FACE	789:796	DSA-FACE	789:796	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	5	theme	Assisted	750:757	arg1	Electrophoresis					772:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis	716:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:797	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	2	6	theme	low-grade	301:309	arg1	inflammation					311:322	chronic low-grade inflammation	293:322	chronic low-grade inflammation	293:322	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	3	7	with	subjects	635:642	arg1	episode					591:597	an acute depressive episode	571:597	an acute depressive episode	571:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	4	8	theme	structures	1078:1087	arg1	levels					1051:1056	the serum levels	1041:1056	the serum levels of several N-glycan structures	1041:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	3	9	theme	Sequencer	720:728	arg1	DSA-FACE					789:796	DSA-FACE	789:796	DSA-FACE	789:796	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	9	theme	Sequencer	720:728	arg1	Electrophoresis					772:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis	716:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:797	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	8	10	theme	serum	1747:1751	arg1	proteins					1753:1760	serum proteins	1747:1760	serum proteins	1747:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	3	11	theme	patients	557:564	arg1	cohort					537:542	a study cohort	529:542	a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years	529:671	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	2	12	from	changes	483:489	arg1	processes					507:515	inflammatory processes	494:515	inflammatory processes	494:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	6	13	theme	MDD	1317:1319	arg1	patients					1321:1328	MDD patients	1317:1328	MDD patients	1317:1328	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	4	14	theme	significant	1014:1024	arg1	differences					1026:1036	significant differences	1014:1036	significant differences in the serum levels of several N-glycan structures	1014:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	3	15	theme	depressive	580:589	arg1	episode					591:597	an acute depressive episode	571:597	an acute depressive episode	571:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	16	dep	-α	880:881	arg1	factor					868:873	tumor necrosis factor	853:873	tumor necrosis factor	853:873	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	1	17	theme	short	116:120	arg1	chains					122:127	short chains	116:127	short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes	116:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	1	17	theme	short	116:120	arg1	Glycans					104:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	3	18	theme	- 6	848:850	arg1	levels					822:827	the serum levels	812:827	the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP)	812:910	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	7	19	theme	confounders	1593:1603	arg1	size					1558:1561	The relatively small sample size	1530:1561	The relatively small sample size	1530:1561	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	7	19	theme	confounders	1593:1603	arg1	presence					1571:1578	the presence	1567:1578	the presence of potential confounders	1567:1603	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	8	20	dep	CONCLUSION	1639:1648	arg1	offer					1662:1666	offer	1662:1666	offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD	1662:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	3	21	theme	control	627:633	arg1	subjects					635:642	21 non-depressed female control subjects	603:642	21 non-depressed female control subjects	603:642	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	8	22	gly	N-glycosylation	1720:1734	arg1	proteins					1753:1760	serum proteins	1747:1760	serum proteins	1747:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	5	23	theme	sexual	1290:1295	arg1	abuse					1297:1301	childhood sexual abuse	1280:1301	childhood sexual abuse	1280:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	24	theme	non-depressed	606:618	arg1	subjects					635:642	21 non-depressed female control subjects	603:642	21 non-depressed female control subjects	603:642	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	0	25	theme	Major	66:70	arg1	Disorder					83:90	Major Depressive Disorder	66:90	Major Depressive Disorder	66:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	5	26	theme	N-glycan	1115:1122	arg1	profile					1124:1130	the serum N-glycan profile	1105:1130	the serum N-glycan profile	1105:1130	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	8	27	theme	unrecognized	1782:1793	arg1	level					1795:1799	a so far unrecognized level	1773:1799	a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD	1773:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	4	28	from	differences	1026:1036	arg1	levels					1051:1056	the serum levels	1041:1056	the serum levels of several N-glycan structures	1041:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	3	29	theme	tumor	853:857	arg1	factor					868:873	tumor necrosis factor	853:873	tumor necrosis factor	853:873	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	0	30	from	Alterations	0:10	arg1	patients					52:59	female patients	45:59	female patients with Major Depressive Disorder	45:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	6	31	theme	IL-6	1354:1357	arg1	levels					1344:1349	higher levels	1337:1349	higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients	1337:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	32	theme	-α	880:881	arg1	levels					822:827	the serum levels	812:827	the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP)	812:910	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	7	33	theme	small	1545:1549	arg1	size					1558:1561	The relatively small sample size	1530:1561	The relatively small sample size	1530:1561	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	8	34	theme	alterations	1815:1825	arg1	level					1795:1799	a so far unrecognized level	1773:1799	a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD	1773:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	3	35	with	patients	557:564	arg1	episode					591:597	an acute depressive episode	571:597	an acute depressive episode	571:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	5	36	theme	symptom	1164:1170	arg1	severity					1172:1179	depressive symptom severity	1153:1179	depressive symptom severity	1153:1179	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	2	37	theme	proteins	439:446	arg1	pattern					422:428	the N-glycosylation pattern	402:428	the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes	402:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	3	38	theme	C-reactive	887:896	arg1	protein					898:904	C-reactive protein	887:904	C-reactive protein (CRP)	887:910	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	38	theme	C-reactive	887:896	arg1	CRP					907:909	CRP	907:909	CRP	907:909	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	2	39	theme	associated	472:481	arg1	changes					483:489	associated changes	472:489	associated changes in inflammatory processes	472:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	2	40	theme	N-glycosylation	406:420	arg1	pattern					422:428	the N-glycosylation pattern	402:428	the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes	402:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	0	41	theme	N-glycan	25:32	arg1	profile					34:40	the serum N-glycan profile	15:40	the serum N-glycan profile	15:40	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	3	42	theme	chemiluminescence	915:931	arg1	immunoassays					933:944	chemiluminescence immunoassays	915:944	chemiluminescence immunoassays	915:944	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	43	theme	aged	644:647	arg1	patients					557:564	21 female patients	547:564	21 female patients with an acute depressive episode	547:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	6	44	theme	CRP	1382:1384	arg1	levels					1386:1391	higher CRP levels	1375:1391	higher CRP levels	1375:1391	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	1	45	theme	wide	203:206	arg1	range					208:212	a wide range	201:212	a wide range of inflammatory processes	201:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	4	46	theme	N-glycan	1069:1076	arg1	structures					1078:1087	several N-glycan structures	1061:1087	several N-glycan structures	1061:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	8	47	from	differences	1701:1711	arg1	pattern					1736:1742	the N-glycosylation pattern	1716:1742	the N-glycosylation pattern of serum proteins	1716:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	2	48	theme	Depressive	362:371	arg1	Disorder					373:380	Major Depressive Disorder	356:380	Major Depressive Disorder	356:380	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	6	49	theme	serum	1453:1457	arg1	profile					1468:1474	the serum N-glycan profile	1449:1474	the serum N-glycan profile as those characteristic for MDD patients	1449:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	50	theme	serum	690:694	arg1	profile					705:711	the serum N-glycan profile	686:711	the serum N-glycan profile	686:711	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	1	51	theme	processes	230:238	arg1	range					208:212	a wide range	201:212	a wide range of inflammatory processes	201:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	7	52	dep	BMI	1612:1614	arg1	e.g.					1606:1609	e.g.	1606:1609	e.g.	1606:1609	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	4	53	theme	serum	1045:1049	arg1	levels					1051:1056	the serum levels	1041:1056	the serum levels of several N-glycan structures	1041:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	3	54	from	METHODS	518:524	arg1	cohort					537:542	a study cohort	529:542	a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years	529:671	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	6	55	theme	similar	1426:1432	arg1	alterations					1434:1444	similar alterations	1426:1444	similar alterations in the serum N-glycan profile as those characteristic for MDD patients	1426:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	56	theme	DNA	716:718	arg1	DSA-FACE					789:796	DSA-FACE	789:796	DSA-FACE	789:796	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	56	theme	DNA	716:718	arg1	Electrophoresis					772:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis	716:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:797	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	0	57	with	patients	52:59	arg1	Disorder					83:90	Major Depressive Disorder	66:90	Major Depressive Disorder	66:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	3	58	theme	study	531:535	arg1	cohort					537:542	a study cohort	529:542	a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years	529:671	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	5	59	from	patients	1253:1260	arg1	pronounced					1235:1244	pronounced	1235:1244	pronounced	1235:1244	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	60	theme	Carbohydrate	759:770	arg1	DSA-FACE					789:796	DSA-FACE	789:796	DSA-FACE	789:796	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	60	theme	Carbohydrate	759:770	arg1	Electrophoresis					772:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis	716:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:797	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	6	61	theme	higher	1337:1342	arg1	levels					1344:1349	higher levels	1337:1349	higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients	1337:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	62	theme	female	550:555	arg1	patients					557:564	21 female patients	547:564	21 female patients with an acute depressive episode	547:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	8	63	theme	specific	1692:1699	arg1	differences					1701:1711	specific differences	1692:1711	specific differences in the N-glycosylation pattern of serum proteins	1692:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	6	64	theme	MDD	1504:1506	arg1	patients					1508:1515	MDD patients	1504:1515	MDD patients	1504:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	3	65	theme	Adapted-Fluorophore	730:748	arg1	DSA-FACE					789:796	DSA-FACE	789:796	DSA-FACE	789:796	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	65	theme	Adapted-Fluorophore	730:748	arg1	Electrophoresis					772:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis	716:786	DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:797	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	5	66	from	pronounced	1235:1244	arg1	patients					1253:1260	MDD patients	1249:1260	MDD patients with a history of childhood sexual abuse	1249:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	67	theme	acute	574:578	arg1	episode					591:597	an acute depressive episode	571:597	an acute depressive episode	571:597	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	8	68	theme	N-glycosylation	1720:1734	arg1	pattern					1736:1742	the N-glycosylation pattern	1716:1742	the N-glycosylation pattern of serum proteins	1716:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	7	69	theme	potential	1583:1591	arg1	confounders					1593:1603	potential confounders	1583:1603	potential confounders	1583:1603	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	1	70	gly	glycoproteins	154:166	arg1	glycoproteins					154:166	glycoproteins	154:166	glycoproteins that are known to be involved in a wide range of inflammatory processes	154:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	3	71	theme	female	620:625	arg1	subjects					635:642	21 non-depressed female control subjects	603:642	21 non-depressed female control subjects	603:642	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	2	72	theme	chronic	293:299	arg1	inflammation					311:322	chronic low-grade inflammation	293:322	chronic low-grade inflammation	293:322	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	5	73	theme	childhood	1280:1288	arg1	abuse					1297:1301	childhood sexual abuse	1280:1301	childhood sexual abuse	1280:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	74	theme	serum	816:820	arg1	levels					822:827	the serum levels	812:827	the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP)	812:910	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	6	75	theme	higher	1375:1380	arg1	levels					1386:1391	higher CRP levels	1375:1391	higher CRP levels	1375:1391	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	7	76	dep	size	1558:1561	arg1	BMI					1612:1614	BMI	1612:1614	BMI	1612:1614	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	7	76	dep	size	1558:1561	arg1	LIMITATIONS					1518:1528	LIMITATIONS	1518:1528	LIMITATIONS	1518:1528	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	7	76	dep	size	1558:1561	arg1	medication					1626:1635	medication	1626:1635	medication	1626:1635	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	7	76	dep	size	1558:1561	arg1	smoking					1617:1623	smoking	1617:1623	smoking	1617:1623	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	8	77	theme	proteins	1753:1760	arg1	pattern					1736:1742	the N-glycosylation pattern	1716:1742	the N-glycosylation pattern of serum proteins	1716:1760	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	1	78	theme	saccharides	132:142	arg1	chains					122:127	short chains	116:127	short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes	116:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	1	78	theme	saccharides	132:142	arg1	Glycans					104:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	2	79	theme	inflammatory	494:505	arg1	processes					507:515	inflammatory processes	494:515	inflammatory processes	494:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	5	80	theme	MDD	1249:1251	arg1	patients					1253:1260	MDD patients	1249:1260	MDD patients with a history of childhood sexual abuse	1249:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	0	81	theme	Depressive	72:81	arg1	Disorder					83:90	Major Depressive Disorder	66:90	Major Depressive Disorder	66:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	5	82	theme	serum	1109:1113	arg1	profile					1124:1130	the serum N-glycan profile	1105:1130	the serum N-glycan profile	1105:1130	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	7	83	theme	sample	1551:1556	arg1	size					1558:1561	The relatively small sample size	1530:1561	The relatively small sample size	1530:1561	LIMITATIONS The relatively small sample size and the presence of potential confounders (e.g., BMI, smoking, medication).					
29529546	5	84	with	patients	1253:1260	arg1	history					1269:1275	a history	1267:1275	a history of childhood sexual abuse	1267:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	85	theme	necrosis	859:866	arg1	factor					868:873	tumor necrosis factor	853:873	tumor necrosis factor	853:873	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	2	86	gly	N-glycosylation	406:420	arg1	proteins					439:446	serum proteins	433:446	serum proteins that might be linked to associated changes in inflammatory processes	433:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	2	87	attach	linked	462:467	arg1	changes					483:489	associated changes	472:489	associated changes in inflammatory processes	472:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	2	87	attach	linked	462:467	arg2	proteins					439:446	serum proteins	433:446	serum proteins that might be linked to associated changes in inflammatory processes	433:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	5	88	from	Alterations	1090:1100	arg1	profile					1124:1130	the serum N-glycan profile	1105:1130	the serum N-glycan profile	1105:1130	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	8	89	theme	biological	1804:1813	arg1	alterations					1815:1825	biological alterations	1804:1825	biological alterations that might be involved in the immune changes associated with MDD	1804:1890	CONCLUSION The results offer the first evidence that specific differences in the N-glycosylation pattern of serum proteins constitute a so far unrecognized level of biological alterations that might be involved in the immune changes associated with MDD.					
29529546	4	90	theme	MDD	994:996	arg1	patients					998:1005	MDD patients	994:1005	MDD patients	994:1005	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	4	90	theme	MDD	994:996	arg1	RESULTS					964:970	RESULTS	964:970	RESULTS Compared to controls	964:991	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	5	91	theme	abuse	1297:1301	arg1	history					1269:1275	a history	1267:1275	a history of childhood sexual abuse	1267:1301	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	0	92	theme	serum	19:23	arg1	profile					34:40	the serum N-glycan profile	15:40	the serum N-glycan profile	15:40	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	1	93	theme	BACKGROUND	93:102	arg1	chains					122:127	short chains	116:127	short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes	116:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	1	93	theme	BACKGROUND	93:102	arg1	Glycans					104:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans	93:110	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
29529546	2	94	theme	serum	433:437	arg1	proteins					439:446	serum proteins	433:446	serum proteins that might be linked to associated changes in inflammatory processes	433:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	0	95	theme	profile	34:40	arg1	Alterations					0:10	Alterations	0:10	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder	0:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	5	96	theme	exploratory	1185:1195	arg1	analyses					1197:1204	exploratory analyses	1185:1204	exploratory analyses	1185:1204	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	3	97	theme	protein	898:904	arg1	levels					822:827	the serum levels	812:827	the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP)	812:910	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	3	98	theme	subjects	635:642	arg1	cohort					537:542	a study cohort	529:542	a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years	529:671	METHODS In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry.					
29529546	0	99	theme	female	45:50	arg1	patients					52:59	female patients	45:59	female patients with Major Depressive Disorder	45:90	Alterations of the serum N-glycan profile in female patients with Major Depressive Disorder.					
29529546	5	100	theme	depressive	1153:1162	arg1	severity					1172:1179	depressive symptom severity	1153:1179	depressive symptom severity	1153:1179	Alterations in the serum N-glycan profile were associated with depressive symptom severity and exploratory analyses revealed that they were most pronounced in MDD patients with a history of childhood sexual abuse.					
29529546	2	101	with	patients	342:349	arg1	Disorder					373:380	Major Depressive Disorder	356:380	Major Depressive Disorder	356:380	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	6	102	from	alterations	1434:1444	arg1	profile					1468:1474	the serum N-glycan profile	1449:1474	the serum N-glycan profile as those characteristic for MDD patients	1449:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	2	103	from	alterations	387:397	arg1	pattern					422:428	the N-glycosylation pattern	402:428	the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes	402:515	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	6	104	theme	N-glycan	1459:1466	arg1	profile					1468:1474	the serum N-glycan profile	1449:1474	the serum N-glycan profile as those characteristic for MDD patients	1449:1515	Furthermore, MDD patients showed higher levels of IL-6 and a trend for higher CRP levels, which were also associated with similar alterations in the serum N-glycan profile as those characteristic for MDD patients.					
29529546	4	105	theme	several	1061:1067	arg1	structures					1078:1087	several N-glycan structures	1061:1087	several N-glycan structures	1061:1087	RESULTS Compared to controls, MDD patients showed significant differences in the serum levels of several N-glycan structures.					
29529546	2	106	theme	Major	356:360	arg1	Disorder					373:380	Major Depressive Disorder	356:380	Major Depressive Disorder	356:380	As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes.					
29529546	1	107	theme	inflammatory	217:228	arg1	processes					230:238	inflammatory processes	217:238	inflammatory processes	217:238	BACKGROUND Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes.					
31377636	2	0	theme	hydrolyzed	504:513	arg1	gum					533:535	hydrolyzed and oxidized guar gum	504:535	hydrolyzed and oxidized guar gum	504:535	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	0	1	from	interactions	38:49	arg1	composites					81:90	cellulose nanofibril-based composites	54:90	cellulose nanofibril-based composites	54:90	Understanding hemicellulose-cellulose interactions in cellulose nanofibril-based composites.					
31377636	2	2	dep	galactoglucomannan	479:496	arg1	gum					533:535	hydrolyzed and oxidized guar gum	504:535	hydrolyzed and oxidized guar gum	504:535	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	2	dep	galactoglucomannan	479:496	arg1	gum					557:559	guar gum	552:559	guar gum grafted with polyethylene glycol	552:592	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	2	dep	galactoglucomannan	479:496	arg1	GG-g-PEG					595:602	GG-g-PEG	595:602	GG-g-PEG	595:602	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	2	dep	galactoglucomannan	479:496	arg1	GGhydHox					538:545	GGhydHox	538:545	GGhydHox	538:545	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	2	dep	galactoglucomannan	479:496	arg1	GGM					499:501	GGM	499:501	GGM	499:501	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	6	3	theme	hemicellulose-cellulose	1632:1654	arg1	interactions					1656:1667	hemicellulose-cellulose interactions	1632:1667	hemicellulose-cellulose interactions	1632:1667	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	2	4	theme	composite	374:382	arg1	films					384:388	composite films	374:388	composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG)	374:603	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	4	5	theme	adsorbed	1101:1108	arg1	polysaccharides					1110:1124	the adsorbed polysaccharides	1097:1124	the adsorbed polysaccharides	1097:1124	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	3	6	theme	different	893:901	arg1	rates					914:918	different adsorption rates	893:918	different adsorption rates	893:918	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	4	7	from	model	1194:1198	arg1	friction					1230:1237	friction	1230:1237	friction	1230:1237	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	3	8	dep	although	879:886	arg1	with					888:891	with	888:891	with	888:891	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	1	9	theme	Plant-based	93:103	arg1	hemicellulose					136:148	hemicellulose	136:148	hemicellulose	136:148	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	9	theme	Plant-based	93:103	arg1	cellulose					122:130	cellulose	122:130	cellulose	122:130	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	9	theme	Plant-based	93:103	arg1	polysaccharides					105:119	Plant-based polysaccharides	93:119	Plant-based polysaccharides (cellulose and hemicellulose)	93:149	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	9	theme	Plant-based	93:103	arg1	option					174:179	a very interesting option	155:179	a very interesting option for the preparation of sustainable composite materials to replace fossil plastics	155:261	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	3	10	theme	adsorption	903:912	arg1	rates					914:918	different adsorption rates	893:918	different adsorption rates	893:918	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	4	11	theme	dry	1370:1372	arg1	films					1374:1378	dry films	1370:1378	dry films	1370:1378	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	2	12	theme	macroscopic	642:652	arg1	level					654:658	macroscopic level	642:658	macroscopic level	642:658	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	4	13	theme	polyelectrolyte	1172:1186	arg1	model					1194:1198	a polyelectrolyte brush model	1170:1198	a polyelectrolyte brush model	1170:1198	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	5	14	theme	High	1381:1384	arg1	affinity					1386:1393	High affinity	1381:1393	High affinity for cellulose and moderate hydration	1381:1430	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	1	15	theme	hard	310:313	arg1	components					324:333	the hard and soft components	306:333	the hard and soft components	306:333	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	6	16	theme	medical	1763:1769	arg1	applications					1771:1782	medical applications	1763:1782	medical applications	1763:1782	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	2	17	theme	cellulose	398:406	arg1	nanofibrils					408:418	cellulose nanofibrils	398:418	cellulose nanofibrils (CNF)	398:424	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	17	theme	cellulose	398:406	arg1	CNF					421:423	CNF	421:423	CNF	421:423	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	1	18	theme	sustainable	204:214	arg1	materials					226:234	sustainable composite materials	204:234	sustainable composite materials	204:234	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	2	19	theme	guar	528:531	arg1	gum					533:535	hydrolyzed and oxidized guar gum	504:535	hydrolyzed and oxidized guar gum	504:535	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	4	20	theme	force	1029:1033	arg1	experiments					1035:1045	Direct surface and friction force experiments	1001:1045	experiments	1035:1045	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	6	21	theme	different	1739:1747	arg1	packaging					1749:1757	different packaging	1739:1757	different packaging	1739:1757	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	6	22	from	information	1617:1627	arg1	interactions					1656:1667	hemicellulose-cellulose interactions	1632:1667	hemicellulose-cellulose interactions	1632:1667	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	2	23	theme	mechanical	773:782	arg1	properties					784:793	macroscopic mechanical properties	761:793	macroscopic mechanical properties	761:793	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	1	24	theme	composite	216:224	arg1	materials					226:234	sustainable composite materials	204:234	sustainable composite materials	204:234	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	0	25	theme	hemicellulose-cellulose	14:36	arg1	interactions					38:49	hemicellulose-cellulose interactions	14:49	hemicellulose-cellulose interactions in cellulose nanofibril-based composites	14:90	Understanding hemicellulose-cellulose interactions in cellulose nanofibril-based composites.					
31377636	4	26	theme	films	1374:1378	arg1	strength					1358:1365	the strength	1354:1365	the strength of dry films	1354:1378	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	5	27	theme	composites	1542:1551	arg1	properties					1518:1527	the mechanical properties	1503:1527	the mechanical properties of CNF-based composites in wet conditions	1503:1569	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	4	28	theme	Direct	1001:1006	arg1	surface					1008:1014	Direct surface and friction force experiments	1001:1045	surface	1008:1014	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	2	29	theme	macroscopic	761:771	arg1	properties					784:793	macroscopic mechanical properties	761:793	macroscopic mechanical properties	761:793	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	1	30	theme	materials	226:234	arg1	preparation					189:199	the preparation	185:199	the preparation of sustainable composite materials to replace fossil plastics	185:261	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	2	31	theme	unmodified	451:460	arg1	galactoglucomannan					479:496	galactoglucomannan	479:496	galactoglucomannan	479:496	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	31	theme	unmodified	451:460	arg1	polysaccharides					462:476	various modified and unmodified polysaccharides	430:476	various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG)	430:603	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	5	32	theme	wet	1556:1558	arg1	conditions					1560:1569	wet conditions	1556:1569	wet conditions	1556:1569	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	6	33	theme	materials	1725:1733	arg1	development					1689:1699	the development	1685:1699	the development of polysaccharide-based materials for different packaging and medical applications	1685:1782	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	5	34	theme	mechanical	1507:1516	arg1	properties					1518:1527	the mechanical properties	1503:1527	the mechanical properties of CNF-based composites in wet conditions	1503:1569	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	1	35	theme	soft	319:322	arg1	components					324:333	the hard and soft components	306:333	the hard and soft components	306:333	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	6	36	theme	polysaccharide-based	1704:1723	arg1	materials					1725:1733	polysaccharide-based materials	1704:1733	polysaccharide-based materials for different packaging and medical applications	1704:1782	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	3	37	theme	dissipation	969:979	arg1	QCM-D					993:997	QCM-D	993:997	QCM-D	993:997	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	3	37	theme	dissipation	969:979	arg1	monitoring					981:990	dissipation monitoring	969:990	dissipation monitoring (QCM-D)	969:998	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	2	38	theme	various	430:436	arg1	galactoglucomannan					479:496	galactoglucomannan	479:496	galactoglucomannan	479:496	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	38	theme	various	430:436	arg1	polysaccharides					462:476	various modified and unmodified polysaccharides	430:476	various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG)	430:603	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	39	theme	modified	438:445	arg1	galactoglucomannan					479:496	galactoglucomannan	479:496	galactoglucomannan	479:496	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	39	theme	modified	438:445	arg1	polysaccharides					462:476	various modified and unmodified polysaccharides	430:476	various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG)	430:603	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	40	theme	guar	552:555	arg1	gum					557:559	guar gum	552:559	guar gum grafted with polyethylene glycol	552:592	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	3	41	theme	crystal	943:949	arg1	microbalance					951:962	quartz crystal microbalance	936:962	quartz crystal microbalance with dissipation monitoring (QCM-D)	936:998	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	1	42	theme	fossil	247:252	arg1	plastics					254:261	fossil plastics	247:261	fossil plastics	247:261	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	0	43	theme	nanofibril-based	64:79	arg1	composites					81:90	cellulose nanofibril-based composites	54:90	cellulose nanofibril-based composites	54:90	Understanding hemicellulose-cellulose interactions in cellulose nanofibril-based composites.					
31377636	3	44	theme	quartz	936:941	arg1	microbalance					951:962	quartz crystal microbalance	936:962	quartz crystal microbalance with dissipation monitoring (QCM-D)	936:998	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	3	45	with	microbalance	951:962	arg1	QCM-D					993:997	QCM-D	993:997	QCM-D	993:997	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	3	45	with	microbalance	951:962	arg1	monitoring					981:990	dissipation monitoring	969:990	dissipation monitoring (QCM-D)	969:998	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	6	46	theme	work	1592:1595	arg1	results					1576:1582	The results	1572:1582	The results of this work	1572:1595	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	0	47	theme	cellulose	54:62	arg1	composites					81:90	cellulose nanofibril-based composites	54:90	cellulose nanofibril-based composites	54:90	Understanding hemicellulose-cellulose interactions in cellulose nanofibril-based composites.					
31377636	4	48	theme	friction	1020:1027	arg1	experiments					1035:1045	Direct surface and friction force experiments	1001:1045	experiments	1035:1045	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	2	49	theme	composite	714:722	arg1	components					724:733	the composite components	710:733	the composite components at nano/microscale	710:752	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	2	50	from	nano/microscale	738:752	arg1	components					724:733	the composite components	710:733	the composite components at nano/microscale	710:752	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	4	51	theme	brush	1188:1192	arg1	model					1194:1198	a polyelectrolyte brush model	1170:1198	a polyelectrolyte brush model	1170:1198	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	2	52	theme	polyethylene	574:585	arg1	glycol					587:592	polyethylene glycol	574:592	polyethylene glycol	574:592	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	3	53	dep	studied	849:855	arg1	adsorbed					857:864	adsorbed	857:864	adsorbed	857:864	All the polysaccharides studied adsorbed well on CNF, although with different adsorption rates, as measured by quartz crystal microbalance with dissipation monitoring (QCM-D).					
31377636	5	54	theme	CNF-based	1532:1540	arg1	composites					1542:1551	CNF-based composites	1532:1551	CNF-based composites	1532:1551	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	4	55	theme	CNF	1285:1287	arg1	aggregates					1289:1298	CNF aggregates	1285:1298	CNF aggregates	1285:1298	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	4	56	dep	prevent	1277:1283	arg1	enhance					1346:1352	enhance	1346:1352	enhance the strength of dry films	1346:1378	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	4	57	theme	cellulose	1247:1255	arg1	surfaces					1257:1264	cellulose surfaces	1247:1264	cellulose surfaces	1247:1264	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	1	58	theme	interesting	162:172	arg1	option					174:179	a very interesting option	155:179	a very interesting option for the preparation of sustainable composite materials to replace fossil plastics	155:261	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	58	theme	interesting	162:172	arg1	polysaccharides					105:119	Plant-based polysaccharides	93:119	Plant-based polysaccharides (cellulose and hemicellulose)	93:149	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	5	59	theme	important	1449:1457	arg1	requirements					1459:1470	important requirements	1449:1470	important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions	1449:1569	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	4	60	from	reduction	1217:1225	arg1	friction					1230:1237	friction	1230:1237	friction	1230:1237	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	4	61	theme	moderate	1208:1215	arg1	reduction					1217:1225	a moderate reduction	1206:1225	a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films	1206:1378	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	1	62	dep	polysaccharides	105:119	arg1	hemicellulose					136:148	hemicellulose	136:148	hemicellulose	136:148	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	62	dep	polysaccharides	105:119	arg1	cellulose					122:130	cellulose	122:130	cellulose	122:130	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	1	62	dep	polysaccharides	105:119	arg1	polysaccharides					105:119	Plant-based polysaccharides	93:119	Plant-based polysaccharides (cellulose and hemicellulose)	93:149	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	4	63	theme	composite	1307:1315	arg1	formation					1317:1325	composite formation	1307:1325	composite formation	1307:1325	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	6	64	theme	fundamental	1605:1615	arg1	information					1617:1627	fundamental information	1605:1627	fundamental information on hemicellulose-cellulose interactions	1605:1667	The results of this work provide fundamental information on hemicellulose-cellulose interactions and can support the development of polysaccharide-based materials for different packaging and medical applications.					
31377636	2	65	theme	oxidized	519:526	arg1	gum					533:535	hydrolyzed and oxidized guar gum	504:535	hydrolyzed and oxidized guar gum	504:535	In this work, composite films made of cellulose nanofibrils (CNF) and various modified and unmodified polysaccharides (galactoglucomannan, GGM; hydrolyzed and oxidized guar gum, GGhydHox; and guar gum grafted with polyethylene glycol, GG-g-PEG) were characterized from the nano- to macroscopic level to better understand how the interactions between the composite components at nano/microscale affect macroscopic mechanical properties, like toughness and strength.					
31377636	5	66	from	properties	1518:1527	arg1	conditions					1560:1569	wet conditions	1556:1569	wet conditions	1556:1569	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	5	67	theme	moderate	1413:1420	arg1	hydration					1422:1430	moderate hydration	1413:1430	moderate hydration	1413:1430	High affinity for cellulose and moderate hydration were found to be important requirements for polysaccharides to improve the mechanical properties of CNF-based composites in wet conditions.					
31377636	4	68	theme	repulsive	1135:1143	arg1	forces-well					1145:1155	repulsive forces-well	1135:1155	repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films	1135:1378	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	1	69	theme	optimum	272:278	arg1	mechanism					288:296	the optimum bonding mechanism	268:296	the optimum bonding mechanism between the hard and soft components	268:333	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
31377636	4	70	theme	probe	1067:1071	arg1	technique					1073:1081	the colloidal probe technique	1053:1081	the colloidal probe technique	1053:1081	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	4	71	theme	colloidal	1057:1065	arg1	technique					1073:1081	the colloidal probe technique	1053:1081	the colloidal probe technique	1053:1081	Direct surface and friction force experiments using the colloidal probe technique revealed that the adsorbed polysaccharides provided repulsive forces-well described by a polyelectrolyte brush model - and a moderate reduction in friction between cellulose surfaces, which may prevent CNF aggregates during composite formation and, consequently, enhance the strength of dry films.					
31377636	1	72	theme	bonding	280:286	arg1	mechanism					288:296	the optimum bonding mechanism	268:296	the optimum bonding mechanism between the hard and soft components	268:333	Plant-based polysaccharides (cellulose and hemicellulose) are a very interesting option for the preparation of sustainable composite materials to replace fossil plastics, but the optimum bonding mechanism between the hard and soft components is still not well known.					
30846831	2	0	theme	chronic	469:475	arg1	gastritis					477:485	chronic gastritis	469:485	chronic gastritis	469:485	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	7	1	theme	H.	1099:1100	arg1	ability					1115:1121	the H. suis-binding ability	1095:1121	the H. suis-binding ability of the mucins	1095:1135	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	2	2	from	%	418:418	arg1	age					441:443	slaughter age	431:443	slaughter age	431:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	3	3	theme	gastric	692:698	arg1	niche					706:710	the pig gastric mucus niche	684:710	the pig gastric mucus niche	684:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	4	dep	H.	555:556	arg1	suis					558:561	H. suis	555:561	experimental H. suis infection	542:571	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	0	5	theme	inhibiting	83:92	arg1	effect					94:99	the pathogen growth inhibiting effect	63:99	the pathogen growth inhibiting effect	63:99	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	2	6	from	age	441:443	arg1	pigs					423:426	pigs	423:426	pigs at slaughter age	423:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	2	6	from	age	441:443	arg1	%					418:418	60-95%	413:418	60-95% of pigs at slaughter age	413:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	7	7	theme	suis-binding	1102:1113	arg1	ability					1115:1121	the H. suis-binding ability	1095:1121	the H. suis-binding ability of the mucins	1095:1135	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	4	8	from	ability	744:750	arg1	humans					786:791	H. pylori-infected humans	767:791	H. pylori-infected humans	767:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	5	9	theme	growth-inhibiting	865:881	arg1	effect					883:888	the H. suis growth-inhibiting effect	853:888	the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs	853:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	5	10	from	pigs	933:936	arg1	mucins					893:898	mucins	893:898	mucins from both noninfected humans and pigs	893:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	5	10	from	pigs	933:936	arg1	effect					883:888	the H. suis growth-inhibiting effect	853:888	the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs	853:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	1	11	theme	chronic	269:275	arg1	gastritis					277:285	chronic gastritis	269:285	chronic gastritis	269:285	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	0	12	theme	binding	105:111	arg1	avidity					113:119	binding avidity	105:119	binding avidity of gastric mucins	105:137	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	3	13	theme	glycan	663:668	arg1	structures					670:679	H. suis-binding glycan structures	647:679	H. suis-binding glycan structures in the pig gastric mucus niche	647:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	5	14	theme	infected	994:1001	arg1	individuals/pigs					1003:1018	infected individuals/pigs	994:1018	infected individuals/pigs	994:1018	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	4	15	theme	H.	728:729	arg1	lower					796:800	lower	796:800	lower	796:800	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	4	15	theme	H.	728:729	arg1	ability					744:750	the H. suis-binding ability	724:750	the H. suis-binding ability of mucins from H. pylori-infected humans	724:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	3	16	theme	suis-binding	650:661	arg1	structures					670:679	H. suis-binding glycan structures	647:679	H. suis-binding glycan structures in the pig gastric mucus niche	647:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	7	17	dep	impair	1056:1061	arg1	decreasing					1144:1153	decreasing	1144:1153	decreasing the antiprolific activity that mucins can have on H. suis	1144:1211	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	7	17	dep	impair	1056:1061	arg1	decreasing					1084:1093	decreasing	1084:1093	decreasing the H. suis-binding ability of the mucins	1084:1135	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	8	18	theme	stable	1270:1275	arg1	niche					1293:1297	a more stable and inhabitable niche	1263:1297	a more stable and inhabitable niche for H. suis	1263:1309	Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis.					
30846831	2	19	theme	weight	498:503	arg1	gain					505:508	decreased weight gain	488:508	decreased weight gain	488:508	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	3	20	theme	mucus	700:704	arg1	niche					706:710	the pig gastric mucus niche	684:710	the pig gastric mucus niche	684:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	21	from	niche	706:710	arg1	structures					670:679	H. suis-binding glycan structures	647:679	H. suis-binding glycan structures in the pig gastric mucus niche	647:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	21	from	niche	706:710	arg1	amount					637:642	the amount	633:642	the amount of H. suis-binding glycan structures in the pig gastric mucus niche	633:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	9	22	theme	therapeutic	1435:1445	arg1	benefits					1447:1454	therapeutic benefits	1435:1454	therapeutic benefits	1435:1454	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	1	23	theme	prevalent	170:178	arg1	suis					153:156	Helicobacter suis	140:156	Helicobacter suis	140:156	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	23	theme	prevalent	170:178	arg1	species					217:223	the most prevalent non-Helicobacter pylori Helicobacter species	161:223	the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach	161:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	2	24	theme	decreased	488:496	arg1	gain					505:508	decreased weight gain	488:508	decreased weight gain	488:508	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	2	25	theme	slaughter	431:439	arg1	age					441:443	slaughter age	431:443	slaughter age	431:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	9	26	theme	importance	1330:1339	arg1	likely					1320:1325	likely	1320:1325	likely	1320:1325	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	1	27	theme	non-Helicobacter	180:195	arg1	suis					153:156	Helicobacter suis	140:156	Helicobacter suis	140:156	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	27	theme	non-Helicobacter	180:195	arg1	species					217:223	the most prevalent non-Helicobacter pylori Helicobacter species	161:223	the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach	161:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	0	28	theme	suis	13:16	arg1	infection					18:26	Helicobacter suis infection	0:26	Helicobacter suis infection	0:26	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	3	29	theme	pig	688:690	arg1	niche					706:710	the pig gastric mucus niche	684:710	the pig gastric mucus niche	684:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	4	30	theme	noninfected	815:825	arg1	individuals					827:837	noninfected individuals	815:837	noninfected individuals	815:837	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	1	31	theme	pylori	197:202	arg1	suis					153:156	Helicobacter suis	140:156	Helicobacter suis	140:156	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	31	theme	pylori	197:202	arg1	species					217:223	the most prevalent non-Helicobacter pylori Helicobacter species	161:223	the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach	161:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	0	32	theme	Helicobacter	0:11	arg1	infection					18:26	Helicobacter suis infection	0:26	Helicobacter suis infection	0:26	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	1	33	theme	peptic	288:293	arg1	disease					301:307	peptic ulcer disease	288:307	peptic ulcer disease	288:307	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	7	34	theme	mucus	1067:1071	arg1	barrier					1073:1079	the mucus barrier	1063:1079	the mucus barrier	1063:1079	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	5	35	from	effect	883:888	arg1	humans					922:927	noninfected humans	910:927	noninfected humans	910:927	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	5	35	from	effect	883:888	arg1	pigs					933:936	pigs	933:936	pigs	933:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	5	36	theme	noninfected	910:920	arg1	humans					922:927	noninfected humans	910:927	noninfected humans	910:927	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	1	37	theme	Helicobacter	204:215	arg1	suis					153:156	Helicobacter suis	140:156	Helicobacter suis	140:156	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	37	theme	Helicobacter	204:215	arg1	species					217:223	the most prevalent non-Helicobacter pylori Helicobacter species	161:223	the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach	161:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	38	theme	ulcer	295:299	arg1	disease					301:307	peptic ulcer disease	288:307	peptic ulcer disease	288:307	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	0	39	theme	mucins	132:137	arg1	effect					94:99	the pathogen growth inhibiting effect	63:99	the pathogen growth inhibiting effect	63:99	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	0	39	theme	mucins	132:137	arg1	avidity					113:119	binding avidity	105:119	binding avidity of gastric mucins	105:137	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	5	40	from	individuals/pigs	1003:1018	arg1	mucins					982:987	mucins	982:987	mucins from infected individuals/pigs	982:1018	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	8	41	theme	mucus-based	1234:1244	arg1	defenses					1246:1253	these mucus-based defenses	1228:1253	these mucus-based defenses	1228:1253	Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis.					
30846831	2	42	theme	pigs	423:426	arg1	pigs					423:426	pigs	423:426	pigs at slaughter age	423:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	2	42	theme	pigs	423:426	arg1	%					418:418	60-95%	413:418	60-95% of pigs at slaughter age	413:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	0	43	theme	gastric	124:130	arg1	mucins					132:137	gastric mucins	124:137	gastric mucins	124:137	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	5	44	dep	H.	857:858	arg1	suis					860:863	H. suis	857:863	H. suis	857:863	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	2	45	theme	%	418:418	arg1	mucosa					403:408	the gastric mucosa	391:408	the gastric mucosa of 60-95% of pigs at slaughter age	391:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	7	46	theme	antiprolific	1159:1170	arg1	activity					1172:1179	the antiprolific activity	1155:1179	the antiprolific activity that mucins can have on H. suis	1155:1211	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	4	47	from	humans	786:791	arg1	lower					796:800	lower	796:800	lower	796:800	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	4	47	from	humans	786:791	arg1	mucins					755:760	mucins	755:760	mucins from H. pylori-infected humans	755:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	4	47	from	humans	786:791	arg1	ability					744:750	the H. suis-binding ability	724:750	the H. suis-binding ability of mucins from H. pylori-infected humans	724:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	8	48	dep	H.	1303:1304	arg1	suis					1306:1309	H. suis	1303:1309	H. suis	1303:1309	Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis.					
30846831	7	49	theme	mucins	1130:1135	arg1	ability					1115:1121	the H. suis-binding ability	1095:1121	the H. suis-binding ability of the mucins	1095:1135	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	7	50	dep	H.	1205:1206	arg1	suis					1208:1211	H. suis	1205:1211	H. suis	1205:1211	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	4	51	theme	pylori-infected	770:784	arg1	humans					786:791	H. pylori-infected humans	767:791	H. pylori-infected humans	767:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	1	52	theme	human	232:236	arg1	stomach					238:244	the human stomach	228:244	the human stomach	228:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	3	53	from	structures	670:679	arg1	niche					706:710	the pig gastric mucus niche	684:710	the pig gastric mucus niche	684:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	1	54	theme	gastric	314:320	arg1	lymphoma					363:370	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	9	55	contain	have	1430:1433	arg1	impairments					1414:1424	these impairments	1408:1424	these impairments	1408:1424	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	9	55	contain	have	1430:1433	arg2	benefits					1447:1454	therapeutic benefits	1435:1454	therapeutic benefits	1435:1454	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	3	56	from	amount	637:642	arg1	niche					706:710	the pig gastric mucus niche	684:710	the pig gastric mucus niche	684:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	57	theme	experimental	542:553	arg1	infection					563:571	experimental H. suis infection	542:571	experimental H. suis infection	542:571	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	5	58	dep	growth-inhibiting	865:881	arg1	H.					857:858	H.	857:858	H.	857:858	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	8	59	theme	inhabitable	1281:1291	arg1	niche					1293:1297	a more stable and inhabitable niche	1263:1297	a more stable and inhabitable niche for H. suis	1263:1309	Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis.					
30846831	1	60	theme	mucosa-associated	322:338	arg1	MALT					357:360	MALT	357:360	MALT	357:360	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	60	theme	mucosa-associated	322:338	arg1	tissue					349:354	mucosa-associated lymphoid tissue	322:354	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	3	61	theme	structures	670:679	arg1	structures					670:679	H. suis-binding glycan structures	647:679	H. suis-binding glycan structures in the pig gastric mucus niche	647:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	61	theme	structures	670:679	arg1	amount					637:642	the amount	633:642	the amount of H. suis-binding glycan structures in the pig gastric mucus niche	633:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	62	theme	H.	555:556	arg1	infection					563:571	experimental H. suis infection	542:571	experimental H. suis infection	542:571	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	1	63	from	species	217:223	arg1	stomach					238:244	the human stomach	228:244	the human stomach	228:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	5	64	from	humans	922:927	arg1	mucins					893:898	mucins	893:898	mucins from both noninfected humans and pigs	893:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	5	64	from	humans	922:927	arg1	effect					883:888	the H. suis growth-inhibiting effect	853:888	the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs	853:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	1	65	theme	lymphoid	340:347	arg1	MALT					357:360	MALT	357:360	MALT	357:360	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	65	theme	lymphoid	340:347	arg1	tissue					349:354	mucosa-associated lymphoid tissue	322:354	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	3	66	theme	H.	647:648	arg1	structures					670:679	H. suis-binding glycan structures	647:679	H. suis-binding glycan structures in the pig gastric mucus niche	647:710	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	3	67	theme	mucin	585:589	arg1	composition					591:601	the mucin composition	581:601	the mucin composition	581:601	Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche.					
30846831	5	68	theme	mucins	893:898	arg1	effect					883:888	the H. suis growth-inhibiting effect	853:888	the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs	853:936	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	9	69	theme	long-term	1345:1353	arg1	colonization					1355:1366	long-term colonization	1345:1366	long-term colonization	1345:1366	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	2	70	dep	H.	373:374	arg1	suis					376:379	H. suis	373:379	H. suis	373:379	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	9	71	theme	infection	1383:1391	arg1	importance					1330:1339	importance	1330:1339	importance for long-term colonization and outcome of infection	1330:1391	This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.					
30846831	5	72	theme	growth-enhancing	955:970	arg1	effect					972:977	a growth-enhancing effect	953:977	a growth-enhancing effect	953:977	Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs.					
30846831	7	73	contain	have	1197:1200	arg1	mucins					1186:1191	mucins	1186:1191	mucins	1186:1191	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	7	73	contain	have	1197:1200	arg2	activity					1172:1179	the antiprolific activity	1155:1179	the antiprolific activity that mucins can have on H. suis	1155:1211	infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis.					
30846831	1	74	theme	Helicobacter	140:151	arg1	species					217:223	the most prevalent non-Helicobacter pylori Helicobacter species	161:223	the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach	161:244	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	1	74	theme	Helicobacter	140:151	arg1	suis					153:156	Helicobacter suis	140:156	Helicobacter suis	140:156	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	4	75	theme	mucins	755:760	arg1	lower					796:800	lower	796:800	lower	796:800	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	4	75	theme	mucins	755:760	arg1	ability					744:750	the H. suis-binding ability	724:750	the H. suis-binding ability of mucins from H. pylori-infected humans	724:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	0	76	theme	growth	76:81	arg1	effect					94:99	the pathogen growth inhibiting effect	63:99	the pathogen growth inhibiting effect	63:99	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	8	77	theme	defenses	1246:1253	arg1	Inhibition					1214:1223	Inhibition	1214:1223	Inhibition of these mucus-based defenses	1214:1253	Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis.					
30846831	4	78	theme	H.	767:768	arg1	humans					786:791	H. pylori-infected humans	767:791	H. pylori-infected humans	767:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	2	79	theme	gastric	395:401	arg1	mucosa					403:408	the gastric mucosa	391:408	the gastric mucosa of 60-95% of pigs at slaughter age	391:443	H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers.					
30846831	0	80	theme	pathogen	67:74	arg1	effect					94:99	the pathogen growth inhibiting effect	63:99	the pathogen growth inhibiting effect	63:99	Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.					
30846831	1	81	theme	tissue	349:354	arg1	lymphoma					363:370	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	gastric mucosa-associated lymphoid tissue (MALT) lymphoma	314:370	Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.					
30846831	4	82	theme	suis-binding	731:742	arg1	lower					796:800	lower	796:800	lower	796:800	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
30846831	4	82	theme	suis-binding	731:742	arg1	ability					744:750	the H. suis-binding ability	724:750	the H. suis-binding ability of mucins from H. pylori-infected humans	724:791	Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals.					
31494931	2	0	link	N-linked	373:380	arg1	structures					398:407	hybrid N-linked oligosaccharide structures	366:407	hybrid N-linked oligosaccharide structures	366:407	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	1	1	theme	structure	189:197	arg1	alteration					159:168	Abnormal alteration	150:168	Abnormal alteration of N-glycosylation structure	150:197	Abnormal alteration of N-glycosylation structure contributes to glioma progression.					
31494931	6	2	theme	glioma	695:700	arg1	proliferation					707:719	glioma cell proliferation	695:719	glioma cell proliferation	695:719	Knockdown of MGAT1 inhibits glioma cell proliferation and migration.					
31494931	4	3	theme	brain	568:572	arg1	tissues					574:580	normal brain tissues	561:580	normal brain tissues	561:580	Here, we found that the expression of MGAT1 is higher in glioblastoma compared to normal brain tissues.					
31494931	7	4	gly	N-glycosylation	772:786	arg1	transporter					799:809	glucose transporter 1	791:811	glucose transporter 1 (Glut1)	791:819	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	7	4	gly	N-glycosylation	772:786	arg1	Glut1					814:818	Glut1	814:818	Glut1	814:818	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	4	5	theme	normal	561:566	arg1	tissues					574:580	normal brain tissues	561:580	normal brain tissues	561:580	Here, we found that the expression of MGAT1 is higher in glioblastoma compared to normal brain tissues.					
31494931	2	6	theme	cores	344:348	arg1	conversion					307:316	the conversion	303:316	the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures	303:407	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	6	7	theme	MGAT1	680:684	arg1	Knockdown					667:675	Knockdown	667:675	Knockdown of MGAT1	667:684	Knockdown of MGAT1 inhibits glioma cell proliferation and migration.					
31494931	7	8	theme	transporter	799:809	arg1	N-glycosylation					772:786	complex N-glycosylation	764:786	complex N-glycosylation of glucose transporter 1 (Glut1)	764:819	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	3	9	theme	MGAT1	426:430	arg1	function					414:421	The function	410:421	The function of MGAT1 in glioma development	410:452	The function of MGAT1 in glioma development remains largely unknown.					
31494931	2	10	theme	high-mannose	331:342	arg1	cores					344:348	processed high-mannose cores	321:348	processed high-mannose cores	321:348	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	0	11	theme	transporter	131:141	arg1	stability					106:114	the stability	102:114	the stability of the glucose transporter GLUT1	102:147	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	2	12	theme	processed	321:329	arg1	cores					344:348	processed high-mannose cores	321:348	processed high-mannose cores	321:348	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	0	13	theme	glucose	123:129	arg1	transporter					131:141	the glucose transporter GLUT1	119:147	the glucose transporter GLUT1	119:147	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	5	14	theme	serum	624:628	arg1	starvation					630:639	serum starvation	624:639	serum starvation	624:639	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	7	15	theme	Glut1	835:839	arg1	levels					849:854	Glut1 protein levels	835:854	Glut1 protein levels	835:854	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	1	16	theme	glioma	214:219	arg1	progression					221:231	glioma progression	214:231	glioma progression	214:231	Abnormal alteration of N-glycosylation structure contributes to glioma progression.					
31494931	5	17	theme	starvation	630:639	arg1	Inhibition					583:592	Inhibition	583:592	Inhibition of EGFR signalling pathway or serum starvation	583:639	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	2	18	theme	hybrid	366:371	arg1	structures					398:407	hybrid N-linked oligosaccharide structures	366:407	hybrid N-linked oligosaccharide structures	366:407	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	8	19	theme	glioma	951:956	arg1	cells					958:962	glioma cells	951:962	glioma cells	951:962	In summary, our findings indicate that MGAT1 is highly expressed in glioblastoma and promotes glioma cells at least partly through upregulation of Glut1 protein.					
31494931	7	20	theme	glucose	791:797	arg1	transporter					799:809	glucose transporter 1	791:811	glucose transporter 1 (Glut1)	791:819	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	7	20	theme	glucose	791:797	arg1	Glut1					814:818	Glut1	814:818	Glut1	814:818	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	0	21	theme	glioma	43:48	arg1	cell					50:53	glioma cell proliferation and migration	43:81	glioma cell proliferation and migration	43:81	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	5	22	theme	MGAT1	649:653	arg1	expression					655:664	MGAT1 expression	649:664	MGAT1 expression	649:664	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	3	23	theme	glioma	435:440	arg1	development					442:452	glioma development	435:452	glioma development	435:452	The function of MGAT1 in glioma development remains largely unknown.					
31494931	8	24	theme	protein	1010:1016	arg1	upregulation					988:999	upregulation	988:999	upregulation of Glut1 protein	988:1016	In summary, our findings indicate that MGAT1 is highly expressed in glioblastoma and promotes glioma cells at least partly through upregulation of Glut1 protein.					
31494931	3	25	from	function	414:421	arg1	development					442:452	glioma development	435:452	glioma development	435:452	The function of MGAT1 in glioma development remains largely unknown.					
31494931	2	26	theme	essential	285:293	arg1	role					295:298	an essential role	282:298	an essential role	282:298	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	4	27	theme	MGAT1	517:521	arg1	expression					503:512	the expression	499:512	the expression of MGAT1	499:521	Here, we found that the expression of MGAT1 is higher in glioblastoma compared to normal brain tissues.					
31494931	4	27	theme	MGAT1	517:521	arg1	higher					526:531	higher	526:531	higher	526:531	Here, we found that the expression of MGAT1 is higher in glioblastoma compared to normal brain tissues.					
31494931	5	28	theme	EGFR	597:600	arg1	pathway					613:619	EGFR signalling pathway	597:619	EGFR signalling pathway	597:619	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	5	29	theme	signalling	602:611	arg1	pathway					613:619	EGFR signalling pathway	597:619	EGFR signalling pathway	597:619	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	7	30	theme	protein	841:847	arg1	levels					849:854	Glut1 protein levels	835:854	Glut1 protein levels	835:854	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	0	31	dep	cell	50:53	arg1	proliferation					55:67	proliferation	55:67	proliferation	55:67	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	0	31	dep	cell	50:53	arg1	migration					73:81	migration	73:81	migration	73:81	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	5	32	theme	pathway	613:619	arg1	Inhibition					583:592	Inhibition	583:592	Inhibition of EGFR signalling pathway or serum starvation	583:639	Inhibition of EGFR signalling pathway or serum starvation reduces MGAT1 expression.					
31494931	7	33	theme	complex	764:770	arg1	N-glycosylation					772:786	complex N-glycosylation	764:786	complex N-glycosylation of glucose transporter 1 (Glut1)	764:819	Furthermore, MGAT1 promotes complex N-glycosylation of glucose transporter 1 (Glut1) and increases Glut1 protein levels.					
31494931	8	34	theme	Glut1	1004:1008	arg1	protein					1010:1016	Glut1 protein	1004:1016	Glut1 protein	1004:1016	In summary, our findings indicate that MGAT1 is highly expressed in glioblastoma and promotes glioma cells at least partly through upregulation of Glut1 protein.					
31494931	1	35	theme	Abnormal	150:157	arg1	alteration					159:168	Abnormal alteration	150:168	Abnormal alteration of N-glycosylation structure	150:197	Abnormal alteration of N-glycosylation structure contributes to glioma progression.					
31494931	0	36	dep	transporter	131:141	arg1	GLUT1					143:147	GLUT1	143:147	the glucose transporter GLUT1	119:147	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1.					
31494931	2	37	theme	N-linked	373:380	arg1	structures					398:407	hybrid N-linked oligosaccharide structures	366:407	hybrid N-linked oligosaccharide structures	366:407	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	2	38	theme	oligosaccharide	382:396	arg1	structures					398:407	hybrid N-linked oligosaccharide structures	366:407	hybrid N-linked oligosaccharide structures	366:407	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	2	39	theme	N-acetylglucosaminyltransferase	234:264	arg1	I					266:266	N-acetylglucosaminyltransferase I	234:266	N-acetylglucosaminyltransferase I (MGAT1)	234:274	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	2	39	theme	N-acetylglucosaminyltransferase	234:264	arg1	MGAT1					269:273	MGAT1	269:273	MGAT1	269:273	N-acetylglucosaminyltransferase I (MGAT1) plays an essential role in the conversion of processed high-mannose cores into complex or hybrid N-linked oligosaccharide structures.					
31494931	1	40	theme	N-glycosylation	173:187	arg1	structure					189:197	N-glycosylation structure	173:197	N-glycosylation structure	173:197	Abnormal alteration of N-glycosylation structure contributes to glioma progression.					
31494931	6	41	theme	cell	702:705	arg1	proliferation					707:719	glioma cell proliferation	695:719	glioma cell proliferation	695:719	Knockdown of MGAT1 inhibits glioma cell proliferation and migration.					
30423744	2	0	from	effects	390:396	arg1	growth					425:430	biofilm growth	417:430	biofilm growth	417:430	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	7	1	theme	polysaccharide	987:1000	arg1	productions					1002:1012	polysaccharide productions	987:1012	polysaccharide productions	987:1012	Lactic acid and polysaccharide productions were measured to assess biofilm cariogenicity.					
30423744	9	2	theme	control	1298:1304	arg1	composite					1306:1314	control composite	1298:1314	control composite	1298:1314	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	5	3	from	72 h	864:867	arg1	composites					872:881	composites	872:881	composites	872:881	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	12	4	theme	%	1609:1609	arg1	proportion					1581:1590	an overwhelming S. mutans proportion	1555:1590	an overwhelming S. mutans proportion of 92.2% and 91.2%	1555:1609	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	5	5	from	48 h	855:858	arg1	composites					872:881	composites	872:881	composites	872:881	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	11	6	from	biofilm	1491:1497	arg1	composite					1506:1514	the composite	1502:1514	the composite with 3% DMAHDM	1502:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	8	7	theme	real-time	1068:1076	arg1	reaction					1095:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	13	8	theme	anti-biofilm	1760:1771	arg1	properties					1773:1782	potent anti-biofilm properties	1753:1782	potent anti-biofilm properties	1753:1782	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	8	9	theme	chain	1089:1093	arg1	reaction					1095:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	2	10	theme	first	513:517	arg1	time					519:522	the first time	509:522	the first time	509:522	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	8	11	theme	species	1149:1155	arg1	proportion					1130:1139	the proportion	1126:1139	the proportion of each species in the biofilm	1126:1170	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	14	12	theme	oral	1977:1980	arg1	species					1990:1996	oral biofilm species	1977:1996	oral biofilm species	1977:1996	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	1	13	theme	composite	203:211	arg1	restorations					213:224	dental composite restorations	196:224	dental composite restorations	196:224	Recurrent caries often occurs and is a primary reason for the failure of dental composite restorations.					
30423744	8	14	used	used	1108:1111	arg2	reaction					1095:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	2	15	dep	develop	269:275	arg1	1					266:266	1	266:266	1	266:266	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	14	16	theme	cavity	1945:1950	arg1	restorations					1952:1963	tooth cavity restorations	1939:1963	tooth cavity restorations	1939:1963	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	1	17	theme	Recurrent	123:131	arg1	caries					133:138	Recurrent caries	123:138	Recurrent caries	123:138	Recurrent caries often occurs and is a primary reason for the failure of dental composite restorations.					
30423744	12	18	theme	overwhelming	1558:1569	arg1	proportion					1581:1590	an overwhelming S. mutans proportion	1555:1590	an overwhelming S. mutans proportion of 92.2% and 91.2%	1555:1609	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	2	19	theme	study	250:254	arg1	develop					269:275	develop	269:275	develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM)	269:353	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	2	19	theme	study	250:254	arg1	objectives					231:240	The objectives	227:240	The objectives of this study	227:254	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	12	20	theme	mutans	1574:1579	arg1	proportion					1581:1590	an overwhelming S. mutans proportion	1555:1590	an overwhelming S. mutans proportion of 92.2% and 91.2%	1555:1609	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	6	21	theme	Colony-forming	884:897	arg1	CFUs					906:909	CFUs	906:909	CFUs	906:909	Colony-forming units (CFUs), metabolic activity and live/dead staining were evaluated.					
30423744	6	21	theme	Colony-forming	884:897	arg1	units					899:903	Colony-forming units	884:903	Colony-forming units (CFUs)	884:910	Colony-forming units (CFUs), metabolic activity and live/dead staining were evaluated.					
30423744	0	22	from	species	97:103	arg1	biofilms					113:120	oral biofilms	108:120	oral biofilms	108:120	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	3	23	theme	mass	569:572	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	23	theme	mass	569:572	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	23	theme	mass	569:572	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	23	theme	mass	569:572	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	23	theme	mass	569:572	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	23	theme	mass	569:572	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	2	24	from	suppression	402:412	arg1	growth					425:430	biofilm growth	417:430	biofilm growth	417:430	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	14	25	theme	tooth	1939:1943	arg1	restorations					1952:1963	tooth cavity restorations	1939:1963	tooth cavity restorations	1939:1963	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	9	26	theme	DMAHDM-containing	1173:1189	arg1	composite					1191:1199	DMAHDM-containing composite	1173:1199	DMAHDM-containing composite	1173:1199	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	2	27	theme	species	485:491	arg1	composition					493:503	biofilm species composition	477:503	biofilm species composition for the first time	477:522	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	10	28	theme	metabolic	1325:1333	arg1	activity					1335:1342	Biofilm metabolic activity	1317:1342	Biofilm metabolic activity	1317:1342	Biofilm metabolic activity, lactic acid and polysaccharides were decreased substantially, compared to control (p < 0.05).					
30423744	5	29	theme	growing	834:840	arg1	biofilms					842:849	growing biofilms	834:849	growing biofilms	834:849	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	0	30	theme	oral	108:111	arg1	biofilms					113:120	oral biofilms	108:120	oral biofilms	108:120	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	9	31	theme	strong	1207:1212	arg1	function					1227:1234	a strong anti-biofilm function	1205:1234	a strong anti-biofilm function	1205:1234	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	4	32	theme	composite	622:630	arg1	Heliomolar					632:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar served as a comparative control.					
30423744	4	32	theme	composite	622:630	arg1	control					667:673	a comparative control	653:673	a comparative control	653:673	A commercial composite Heliomolar served as a comparative control.					
30423744	0	33	theme	Novel	0:4	arg1	composite					13:21	Novel dental composite	0:21	Novel dental composite with capability	0:37	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	6	34	theme	metabolic	913:921	arg1	activity					923:930	metabolic activity	913:930	metabolic activity	913:930	Colony-forming units (CFUs), metabolic activity and live/dead staining were evaluated.					
30423744	7	35	theme	biofilm	1038:1044	arg1	cariogenicity					1046:1058	biofilm cariogenicity	1038:1058	biofilm cariogenicity	1038:1058	Lactic acid and polysaccharide productions were measured to assess biofilm cariogenicity.					
30423744	11	36	from	composite	1506:1514	arg1	proportion					1473:1482	the cariogenic S. mutans proportion	1448:1482	the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM	1448:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	36	from	composite	1506:1514	arg1	%					1539:1539	19.9%	1535:1539	19.9%	1535:1539	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	5	37	dep	sanguinis	754:762	arg1	sanguinis					768:776	S. sanguinis	765:776	S. sanguinis	765:776	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	9	38	contain	had	1201:1203	arg2	function					1227:1234	a strong anti-biofilm function	1205:1234	a strong anti-biofilm function	1205:1234	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	9	38	contain	had	1201:1203	arg1	composite					1191:1199	DMAHDM-containing composite	1173:1199	DMAHDM-containing composite	1173:1199	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	2	39	theme	antibacterial	376:388	arg1	effects					390:396	its antibacterial effects	372:396	its antibacterial effects	372:396	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	14	40	theme	new	1869:1871	arg1	composite					1880:1888	The new DMAHDM composite	1865:1888	The new DMAHDM composite	1865:1888	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	14	40	theme	new	1869:1871	arg1	promising					1893:1901	promising	1893:1901	promising	1893:1901	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	3	41	theme	%	579:579	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	41	theme	%	579:579	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	41	theme	%	579:579	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	41	theme	%	579:579	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	41	theme	%	579:579	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	41	theme	%	579:579	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	0	42	with	composite	13:21	arg1	capability					28:37	capability	28:37	capability	28:37	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	9	43	theme	2-3	1261:1263	arg1	orders					1265:1270	2-3 orders	1261:1270	2-3 orders of magnitude	1261:1283	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	12	44	theme	%	1658:1658	arg1	DMAHDM					1660:1665	0% DMAHDM	1657:1665	0% DMAHDM	1657:1665	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	3	45	theme	%	592:592	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	45	theme	%	592:592	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	45	theme	%	592:592	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	45	theme	%	592:592	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	45	theme	%	592:592	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	45	theme	%	592:592	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	11	46	theme	mutans	1466:1471	arg1	proportion					1473:1482	the cariogenic S. mutans proportion	1448:1482	the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM	1448:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	46	theme	mutans	1466:1471	arg1	%					1539:1539	19.9%	1535:1539	19.9%	1535:1539	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	1	47	theme	restorations	213:224	arg1	failure					185:191	the failure	181:191	the failure of dental composite restorations	181:224	Recurrent caries often occurs and is a primary reason for the failure of dental composite restorations.					
30423744	2	48	dep	investigate	360:370	arg1	2					357:357	2	357:357	2	357:357	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	5	49	theme	biofilm	678:684	arg1	model					686:690	A biofilm model	676:690	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii)	676:818	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	12	50	theme	commercial	1634:1643	arg1	control					1645:1651	commercial control	1634:1651	commercial control	1634:1651	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	11	51	theme	%	1522:1522	arg1	DMAHDM					1524:1529	3% DMAHDM	1521:1529	3% DMAHDM	1521:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	13	52	theme	species	1811:1817	arg1	composition					1819:1829	the biofilm species composition	1799:1829	the biofilm species composition toward a non-cariogenic tendency	1799:1862	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	5	53	dep	mutans	720:725	arg1	mutans					731:736	S. mutans	728:736	S. mutans	728:736	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	13	54	theme	potent	1753:1758	arg1	properties					1773:1782	potent anti-biofilm properties	1753:1782	potent anti-biofilm properties	1753:1782	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	7	55	theme	Lactic	971:976	arg1	acid					978:981	Lactic acid	971:981	Lactic acid	971:981	Lactic acid and polysaccharide productions were measured to assess biofilm cariogenicity.					
30423744	14	56	from	applications	1907:1918	arg1	range					1930:1934	a wide range	1923:1934	a wide range of tooth cavity restorations	1923:1963	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	1	57	theme	primary	162:168	arg1	reason					170:175	a primary reason	160:175	a primary reason for the failure of dental composite restorations	160:224	Recurrent caries often occurs and is a primary reason for the failure of dental composite restorations.					
30423744	3	58	theme	%	606:606	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	58	theme	%	606:606	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	58	theme	%	606:606	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	58	theme	%	606:606	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	58	theme	%	606:606	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	58	theme	%	606:606	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	11	59	theme	cariogenic	1452:1461	arg1	proportion					1473:1482	the cariogenic S. mutans proportion	1448:1482	the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM	1448:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	59	theme	cariogenic	1452:1461	arg1	%					1539:1539	19.9%	1535:1539	19.9%	1535:1539	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	2	60	theme	bioactive	279:287	arg1	composite					289:297	a bioactive composite	277:297	a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM)	277:353	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	8	61	theme	TaqMan	1061:1066	arg1	reaction					1095:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	12	62	theme	%	1599:1599	arg1	proportion					1581:1590	an overwhelming S. mutans proportion	1555:1590	an overwhelming S. mutans proportion of 92.2% and 91.2%	1555:1609	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	14	63	theme	biofilm	1982:1988	arg1	species					1990:1996	oral biofilm species	1977:1996	oral biofilm species	1977:1996	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	8	64	theme	polymerase	1078:1087	arg1	reaction					1095:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction	1061:1102	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	11	65	from	proportion	1473:1482	arg1	biofilm					1491:1497	the biofilm	1487:1497	the biofilm on the composite with 3% DMAHDM	1487:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	65	from	proportion	1473:1482	arg1	composite					1506:1514	the composite	1502:1514	the composite with 3% DMAHDM	1502:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	14	66	theme	restorations	1952:1963	arg1	range					1930:1934	a wide range	1923:1934	a wide range of tooth cavity restorations	1923:1963	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	8	67	from	proportion	1130:1139	arg1	biofilm					1164:1170	the biofilm	1160:1170	the biofilm	1160:1170	TaqMan real-time polymerase chain reaction was used to determine the proportion of each species in the biofilm.					
30423744	11	68	from	72 h	1442:1445	arg1	proportion					1473:1482	the cariogenic S. mutans proportion	1448:1482	the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM	1448:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	68	from	72 h	1442:1445	arg1	%					1539:1539	19.9%	1535:1539	19.9%	1535:1539	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	13	69	theme	non-cariogenic	1840:1853	arg1	tendency					1855:1862	a non-cariogenic tendency	1838:1862	a non-cariogenic tendency	1838:1862	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	12	70	theme	S.	1571:1572	arg1	proportion					1581:1590	an overwhelming S. mutans proportion	1555:1590	an overwhelming S. mutans proportion of 92.2% and 91.2%	1555:1609	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	0	71	theme	non-cariogenic	82:95	arg1	species					97:103	non-cariogenic species	82:103	non-cariogenic species in oral biofilms	82:120	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	2	72	contain	containing	299:308	arg1	composite					289:297	a bioactive composite	277:297	a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM)	277:353	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	2	72	contain	containing	299:308	arg2	methacrylate					333:344	dimethylaminohexadecyl methacrylate	310:344	dimethylaminohexadecyl methacrylate (DMAHDM)	310:353	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	2	72	contain	containing	299:308	arg2	DMAHDM					347:352	DMAHDM	347:352	DMAHDM	347:352	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	14	73	theme	wide	1925:1928	arg1	range					1930:1934	a wide range	1923:1934	a wide range of tooth cavity restorations	1923:1963	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	13	74	theme	dental	1723:1728	arg1	composite					1730:1738	dental composite	1723:1738	dental composite	1723:1738	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	10	75	dep	decreased	1382:1390	arg1	compared					1407:1414	compared	1407:1414	compared to control (p < 0.05)	1407:1436	Biofilm metabolic activity, lactic acid and polysaccharides were decreased substantially, compared to control (p < 0.05).					
30423744	2	76	theme	biofilm	477:483	arg1	composition					493:503	biofilm species composition	477:503	biofilm species composition for the first time	477:522	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	10	77	theme	Biofilm	1317:1323	arg1	activity					1335:1342	Biofilm metabolic activity	1317:1342	Biofilm metabolic activity	1317:1342	Biofilm metabolic activity, lactic acid and polysaccharides were decreased substantially, compared to control (p < 0.05).					
30423744	6	78	theme	live/dead	936:944	arg1	staining					946:953	live/dead staining	936:953	live/dead staining	936:953	Colony-forming units (CFUs), metabolic activity and live/dead staining were evaluated.					
30423744	5	79	dep	gordonii	797:804	arg1	gordonii					810:817	S. gordonii	807:817	S. gordonii	807:817	A biofilm model consisting of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii) was tested by growing biofilms for 48 h and 72 h on composites.					
30423744	3	80	from	%	573:573	arg1	composite					556:564	a composite	554:564	a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	554:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	0	81	theme	dental	6:11	arg1	composite					13:21	Novel dental composite	0:21	Novel dental composite with capability	0:37	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	9	82	theme	anti-biofilm	1214:1225	arg1	function					1227:1234	a strong anti-biofilm function	1205:1234	a strong anti-biofilm function	1205:1234	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	2	83	theme	biofilm	417:423	arg1	growth					425:430	biofilm growth	417:430	biofilm growth	417:430	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	10	84	theme	lactic	1345:1350	arg1	acid					1352:1355	lactic acid	1345:1355	lactic acid	1345:1355	Biofilm metabolic activity, lactic acid and polysaccharides were decreased substantially, compared to control (p < 0.05).					
30423744	4	85	theme	commercial	611:620	arg1	Heliomolar					632:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar served as a comparative control.					
30423744	4	85	theme	commercial	611:620	arg1	control					667:673	a comparative control	653:673	a comparative control	653:673	A commercial composite Heliomolar served as a comparative control.					
30423744	9	86	theme	biofilm	1246:1252	arg1	CFU					1254:1256	biofilm CFU	1246:1256	biofilm CFU	1246:1256	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	1	87	theme	dental	196:201	arg1	restorations					213:224	dental composite restorations	196:224	dental composite restorations	196:224	Recurrent caries often occurs and is a primary reason for the failure of dental composite restorations.					
30423744	14	88	theme	DMAHDM	1873:1878	arg1	composite					1880:1888	The new DMAHDM composite	1865:1888	The new DMAHDM composite	1865:1888	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	14	88	theme	DMAHDM	1873:1878	arg1	promising					1893:1901	promising	1893:1901	promising	1893:1901	The new DMAHDM composite is promising for applications in a wide range of tooth cavity restorations to modulate oral biofilm species and combat caries.					
30423744	0	89	theme	cariogenic	51:60	arg1	species					62:68	cariogenic species	51:68	cariogenic species	51:68	Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms.					
30423744	2	90	dep	investigate	441:451	arg1	3					438:438	3	438:438	3	438:438	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	13	91	dep	incorporating	1697:1709	arg1	1					1742:1742	1	1742:1742	1	1742:1742	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	13	91	dep	incorporating	1697:1709	arg1	yielded					1745:1751	yielded	1745:1751	yielded potent anti-biofilm properties	1745:1782	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	13	91	dep	incorporating	1697:1709	arg1	2					1786:1786	2	1786:1786	2	1786:1786	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	3	92	theme	%	599:599	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	92	theme	%	599:599	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	92	theme	%	599:599	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	92	theme	%	599:599	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	92	theme	%	599:599	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	92	theme	%	599:599	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	11	93	theme	S.	1463:1464	arg1	proportion					1473:1482	the cariogenic S. mutans proportion	1448:1482	the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM	1448:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	11	93	theme	S.	1463:1464	arg1	%					1539:1539	19.9%	1535:1539	19.9%	1535:1539	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	2	94	theme	dimethylaminohexadecyl	310:331	arg1	DMAHDM					347:352	DMAHDM	347:352	DMAHDM	347:352	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	2	94	theme	dimethylaminohexadecyl	310:331	arg1	methacrylate					333:344	dimethylaminohexadecyl methacrylate	310:344	dimethylaminohexadecyl methacrylate (DMAHDM)	310:353	The objectives of this study were to: (1) develop a bioactive composite containing dimethylaminohexadecyl methacrylate (DMAHDM), (2) investigate its antibacterial effects and suppression on biofilm growth, and (3) investigate its ability to modulate biofilm species composition for the first time.					
30423744	12	95	theme	0	1657:1657	arg1	%					1658:1658	%	1658:1658	%	1658:1658	In contrast, an overwhelming S. mutans proportion of 92.2% and 91.2% existed in biofilms on commercial control and 0% DMAHDM, respectively.					
30423744	4	96	theme	comparative	655:665	arg1	Heliomolar					632:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar	609:641	A commercial composite Heliomolar served as a comparative control.					
30423744	4	96	theme	comparative	655:665	arg1	control					667:673	a comparative control	653:673	a comparative control	653:673	A commercial composite Heliomolar served as a comparative control.					
30423744	3	97	theme	%	586:586	arg1	%					606:606	3%	605:606	3%	605:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	97	theme	%	586:586	arg1	%					579:579	0%	578:579	0%	578:579	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	97	theme	%	586:586	arg1	%					599:599	2.25%	595:599	2.25%	595:599	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	97	theme	%	586:586	arg1	%					573:573	mass%	569:573	mass% of 0%, 0.75%, 1.5%, 2.25% and 3%	569:606	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	97	theme	%	586:586	arg1	%					586:586	0.75%	582:586	0.75%	582:586	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	3	97	theme	%	586:586	arg1	%					592:592	1.5%	589:592	1.5%	589:592	DMAHDM was incorporated into a composite at mass% of 0%, 0.75%, 1.5%, 2.25% and 3%.					
30423744	9	98	theme	magnitude	1275:1283	arg1	orders					1265:1270	2-3 orders	1261:1270	2-3 orders of magnitude	1261:1283	DMAHDM-containing composite had a strong anti-biofilm function, reducing biofilm CFU by 2-3 orders of magnitude, compared to control composite.					
30423744	11	99	theme	3	1521:1521	arg1	%					1522:1522	%	1522:1522	%	1522:1522	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30423744	13	100	theme	biofilm	1803:1809	arg1	species					1811:1817	biofilm species	1803:1817	the biofilm species composition toward a non-cariogenic tendency	1799:1862	In conclusion, incorporating DMAHDM into dental composite: (1) yielded potent anti-biofilm properties; (2) modulated the biofilm species composition toward a non-cariogenic tendency.					
30423744	11	101	with	composite	1506:1514	arg1	DMAHDM					1524:1529	3% DMAHDM	1521:1529	3% DMAHDM	1521:1529	At 72 h, the cariogenic S. mutans proportion in the biofilm on the composite with 3% DMAHDM was 19.9%.					
30074767	0	0	theme	Simple	71:76	arg1	Method					78:83	a Simple Method	69:83	a Simple Method for Dane Particle Enrichment	69:112	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	12	1	theme	clinical	1388:1395	arg1	tests					1397:1401	clinical tests	1388:1401	clinical tests	1388:1401	We measured HBsAg titer and viral DNA load in each fraction using clinical tests.					
30074767	14	2	from	method	1624:1629	arg1	particle					1592:1599	the intact HBV particle	1577:1599	the intact HBV particle	1577:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	14	3	attach	presented	1528:1536	arg1	study					1514:1518	this study	1509:1518	this study	1509:1518	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	14	3	attach	presented	1528:1536	arg2	we					1520:1521	we	1520:1521	we	1520:1521	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	4	4	theme	infectivity	457:467	arg1	hazardous					423:431	hazardous	423:431	hazardous	423:431	Dane particles are hazardous because of their strong infectivity, unlike SVPs.					
30074767	5	5	theme	glycoprotein	530:541	arg1	envelope					518:525	an envelope	515:525	an envelope of glycoprotein called HBV surface antigen (HBsAg)	515:576	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	10	6	theme	particle	1152:1159	arg1	secretion					1161:1169	Dane particle secretion	1147:1169	Dane particle secretion	1147:1169	Specifically, jacalin, which recognizes O-glycan, showed specific and strong reactivity to the M-HBsAg required for Dane particle secretion.					
30074767	4	7	theme	strong	450:455	arg1	infectivity					457:467	their strong infectivity	444:467	their strong infectivity	444:467	Dane particles are hazardous because of their strong infectivity, unlike SVPs.					
30074767	11	8	theme	HBV	1229:1231	arg1	particles					1233:1241	HBV particles	1229:1241	HBV particles	1229:1241	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	8	9	theme	intact	897:902	arg1	structure					919:927	the intact viral particle structure	893:927	the intact viral particle structure	893:927	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	9	10	theme	lectin	1004:1009	arg1	results					1022:1028	the lectin microarray results	1000:1028	the lectin microarray results	1000:1028	Several typical lectins were chosen from the lectin microarray results.					
30074767	10	11	theme	Dane	1147:1150	arg1	secretion					1161:1169	Dane particle secretion	1147:1169	Dane particle secretion	1147:1169	Specifically, jacalin, which recognizes O-glycan, showed specific and strong reactivity to the M-HBsAg required for Dane particle secretion.					
30074767	6	12	gly	glycosylation	585:597	arg1	secretion					643:651	secretion	643:651	secretion	643:651	HBsAg glycosylation is involved in viral particle formation and secretion.					
30074767	6	12	gly	glycosylation	585:597	arg1	formation					629:637	viral particle formation	614:637	viral particle formation	614:637	HBsAg glycosylation is involved in viral particle formation and secretion.					
30074767	5	13	theme	HBV	550:552	arg1	HBsAg					571:575	HBsAg	571:575	HBsAg	571:575	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	5	13	theme	HBV	550:552	arg1	antigen					562:568	HBV surface antigen	550:568	HBV surface antigen (HBsAg)	550:576	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	7	14	theme	patient	780:786	arg1	serum					788:792	patient serum	780:792	patient serum	780:792	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	2	15	theme	subviral	285:292	arg1	others					260:265	the others	256:265	the others	256:265	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	2	15	theme	subviral	285:292	arg1	SVPs					305:308	SVPs	305:308	SVPs	305:308	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	2	15	theme	subviral	285:292	arg1	particles					294:302	noninfectious subviral particles	271:302	noninfectious subviral particles (SVPs)	271:309	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	0	16	theme	Particle	94:101	arg1	Enrichment					103:112	Dane Particle Enrichment	89:112	Dane Particle Enrichment	89:112	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	9	17	theme	microarray	1011:1020	arg1	results					1022:1028	the lectin microarray results	1000:1028	the lectin microarray results	1000:1028	Several typical lectins were chosen from the lectin microarray results.					
30074767	5	18	theme	surface	554:560	arg1	HBsAg					571:575	HBsAg	571:575	HBsAg	571:575	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	5	18	theme	surface	554:560	arg1	antigen					562:568	HBV surface antigen	550:568	HBV surface antigen (HBsAg)	550:576	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	2	19	theme	Dane	237:240	arg1	particles					242:250	Dane particles	237:250	Dane particles	237:250	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	7	20	theme	serum	788:792	arg1	aliquots					768:775	small aliquots	762:775	small aliquots of patient serum	762:792	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	3	21	theme	higher	376:381	arg1	abundance					343:351	abundance	343:351	abundance	343:351	In blood, SVPs are detected in abundance, about 1000-10000 fold higher than Dane particles.					
30074767	0	22	theme	Dane	89:92	arg1	Enrichment					103:112	Dane Particle Enrichment	89:112	Dane Particle Enrichment	89:112	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	11	23	theme	unbound	1284:1290	arg1	fractions					1292:1300	jacalin-bound and unbound fractions	1266:1300	jacalin-bound and unbound fractions from patient serum	1266:1319	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	8	24	theme	nonreducing	935:945	arg1	conditions					947:956	nonreducing conditions	935:956	nonreducing conditions	935:956	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	7	25	theme	novel	686:690	arg1	method					713:718	a novel and highly sensitive method	684:718	a novel and highly sensitive method for viral glycan profiling of HBsAg	684:754	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	8	26	theme	microarray	806:815	arg1	system					817:822	Our lectin microarray system	795:822	Our lectin microarray system	795:822	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	0	27	theme	Sensitive	7:15	arg1	Profiling					24:32	Highly Sensitive Glycan Profiling	0:32	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles	0:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	11	28	from	serum	1315:1319	arg1	fractions					1292:1300	jacalin-bound and unbound fractions	1266:1300	jacalin-bound and unbound fractions from patient serum	1266:1319	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	2	29	theme	noninfectious	271:283	arg1	others					260:265	the others	256:265	the others	256:265	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	2	29	theme	noninfectious	271:283	arg1	SVPs					305:308	SVPs	305:308	SVPs	305:308	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	2	29	theme	noninfectious	271:283	arg1	particles					294:302	noninfectious subviral particles	271:302	noninfectious subviral particles (SVPs)	271:309	The virions are called Dane particles and the others are noninfectious subviral particles (SVPs).					
30074767	8	30	theme	lectin	799:804	arg1	system					817:822	Our lectin microarray system	795:822	Our lectin microarray system	795:822	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	1	31	theme	double-stranded	144:158	arg1	virus					127:131	Hepatitis B virus	115:131	Hepatitis B virus (HBV)	115:137	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	31	theme	double-stranded	144:158	arg1	virus					164:168	a double-stranded DNA virus	142:168	a double-stranded DNA virus composed of three types of viral particles	142:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	14	32	theme	HBV	1588:1590	arg1	particle					1592:1599	the intact HBV particle	1577:1599	the intact HBV particle	1577:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	7	33	theme	glycan	730:735	arg1	profiling					737:745	viral glycan profiling	724:745	viral glycan profiling of HBsAg	724:754	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	11	34	theme	jacalin-bound	1266:1278	arg1	fractions					1292:1300	jacalin-bound and unbound fractions	1266:1300	jacalin-bound and unbound fractions from patient serum	1266:1319	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	14	35	theme	simple	1617:1622	arg1	method					1624:1629	an easy and simple method	1605:1629	an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation	1605:1698	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	6	36	theme	particle	620:627	arg1	formation					629:637	viral particle formation	614:637	viral particle formation	614:637	HBsAg glycosylation is involved in viral particle formation and secretion.					
30074767	1	37	theme	DNA	160:162	arg1	virus					127:131	Hepatitis B virus	115:131	Hepatitis B virus (HBV)	115:137	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	37	theme	DNA	160:162	arg1	virus					164:168	a double-stranded DNA virus	142:168	a double-stranded DNA virus composed of three types of viral particles	142:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	10	38	theme	strong	1101:1106	arg1	reactivity					1108:1117	specific and strong reactivity	1088:1117	specific and strong reactivity	1088:1117	Specifically, jacalin, which recognizes O-glycan, showed specific and strong reactivity to the M-HBsAg required for Dane particle secretion.					
30074767	8	39	theme	particle	910:917	arg1	structure					919:927	the intact viral particle structure	893:927	the intact viral particle structure	893:927	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	12	40	theme	DNA	1356:1358	arg1	load					1360:1363	viral DNA load	1350:1363	viral DNA load	1350:1363	We measured HBsAg titer and viral DNA load in each fraction using clinical tests.					
30074767	6	41	theme	viral	614:618	arg1	formation					629:637	viral particle formation	614:637	viral particle formation	614:637	HBsAg glycosylation is involved in viral particle formation and secretion.					
30074767	0	42	theme	Glycan	17:22	arg1	Profiling					24:32	Highly Sensitive Glycan Profiling	0:32	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles	0:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	13	43	theme	HBV	1480:1482	arg1	load					1494:1497	the HBV viral DNA load	1476:1497	the HBV viral DNA load	1476:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	11	44	theme	lectin-fractionation	1186:1205	arg1	method					1207:1212	the lectin-fractionation method	1182:1212	the lectin-fractionation method using jacalin	1182:1226	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	14	45	theme	Dane	1641:1644	arg1	particles					1646:1654	Dane particles	1641:1654	Dane particles	1641:1654	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	12	46	theme	viral	1350:1354	arg1	load					1360:1363	viral DNA load	1350:1363	viral DNA load	1350:1363	We measured HBsAg titer and viral DNA load in each fraction using clinical tests.					
30074767	14	47	theme	intact	1581:1586	arg1	particle					1592:1599	the intact HBV particle	1577:1599	the intact HBV particle	1577:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	0	48	theme	Hepatitis	37:45	arg1	Particles					55:63	Hepatitis B Viral Particles	37:63	Hepatitis B Viral Particles	37:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	7	49	theme	viral	724:728	arg1	profiling					737:745	viral glycan profiling	724:745	viral glycan profiling of HBsAg	724:754	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	8	50	theme	viral	904:908	arg1	structure					919:927	the intact viral particle structure	893:927	the intact viral particle structure	893:927	Our lectin microarray system could sensitively profile the glycans exposed on HBV while retaining the intact viral particle structure under nonreducing conditions.					
30074767	7	51	theme	HBsAg	750:754	arg1	profiling					737:745	viral glycan profiling	724:745	viral glycan profiling of HBsAg	724:754	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	3	52	theme	Dane	388:391	arg1	particles					393:401	Dane particles	388:401	Dane particles	388:401	In blood, SVPs are detected in abundance, about 1000-10000 fold higher than Dane particles.					
30074767	7	53	theme	sensitive	703:711	arg1	method					713:718	a novel and highly sensitive method	684:718	a novel and highly sensitive method for viral glycan profiling of HBsAg	684:754	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	14	54	theme	patient	1661:1667	arg1	serum					1669:1673	patient serum	1661:1673	patient serum	1661:1673	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	4	55	theme	Dane	404:407	arg1	particles					409:417	Dane particles	404:417	Dane particles	404:417	Dane particles are hazardous because of their strong infectivity, unlike SVPs.					
30074767	14	56	theme	jacalin	1678:1684	arg1	fractionation					1686:1698	jacalin fractionation	1678:1698	jacalin fractionation	1678:1698	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	5	57	theme	Dane	483:486	arg1	particles					488:496	Dane particles	483:496	Dane particles	483:496	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	13	58	theme	major	1458:1462	arg1	fraction					1464:1471	a major fraction	1456:1471	a major fraction of the HBV viral DNA load	1456:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	0	59	theme	Viral	49:53	arg1	Particles					55:63	Hepatitis B Viral Particles	37:63	Hepatitis B Viral Particles	37:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	7	60	theme	small	762:766	arg1	aliquots					768:775	small aliquots	762:775	small aliquots of patient serum	762:792	In this study, we established a novel and highly sensitive method for viral glycan profiling of HBsAg using small aliquots of patient serum.					
30074767	12	61	theme	HBsAg	1334:1338	arg1	titer					1340:1344	HBsAg titer	1334:1344	HBsAg titer	1334:1344	We measured HBsAg titer and viral DNA load in each fraction using clinical tests.					
30074767	14	62	theme	profiling	1547:1555	arg1	method					1557:1562	a glycan profiling method	1538:1562	a glycan profiling method for HBsAg on the intact HBV particle	1538:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	0	63	theme	B	47:47	arg1	Particles					55:63	Hepatitis B Viral Particles	37:63	Hepatitis B Viral Particles	37:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	3	64	located	detected	331:338	arg1	abundance					343:351	abundance	343:351	abundance	343:351	In blood, SVPs are detected in abundance, about 1000-10000 fold higher than Dane particles.					
30074767	3	64	located	detected	331:338	arg2	SVPs					322:325	SVPs	322:325	SVPs	322:325	In blood, SVPs are detected in abundance, about 1000-10000 fold higher than Dane particles.					
30074767	3	64	located	detected	331:338	arg1	blood					315:319	blood	315:319	blood	315:319	In blood, SVPs are detected in abundance, about 1000-10000 fold higher than Dane particles.					
30074767	14	65	theme	glycan	1540:1545	arg1	method					1557:1562	a glycan profiling method	1538:1562	a glycan profiling method for HBsAg on the intact HBV particle	1538:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	9	66	theme	Several	959:965	arg1	lectins					975:981	Several typical lectins	959:981	Several typical lectins	959:981	Several typical lectins were chosen from the lectin microarray results.					
30074767	10	67	theme	specific	1088:1095	arg1	reactivity					1108:1117	specific and strong reactivity	1088:1117	specific and strong reactivity	1088:1117	Specifically, jacalin, which recognizes O-glycan, showed specific and strong reactivity to the M-HBsAg required for Dane particle secretion.					
30074767	13	68	contain	contained	1446:1454	arg1	fraction					1437:1444	the jacalin-bound fraction	1419:1444	the jacalin-bound fraction	1419:1444	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	13	68	contain	contained	1446:1454	arg2	fraction					1464:1471	a major fraction	1456:1471	a major fraction of the HBV viral DNA load	1456:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	13	69	theme	viral	1484:1488	arg1	load					1494:1497	the HBV viral DNA load	1476:1497	the HBV viral DNA load	1476:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	1	70	theme	Hepatitis	115:123	arg1	HBV					134:136	HBV	134:136	HBV	134:136	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	70	theme	Hepatitis	115:123	arg1	virus					164:168	a double-stranded DNA virus	142:168	a double-stranded DNA virus composed of three types of viral particles	142:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	70	theme	Hepatitis	115:123	arg1	virus					127:131	Hepatitis B virus	115:131	Hepatitis B virus (HBV)	115:137	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	9	71	theme	typical	967:973	arg1	lectins					975:981	Several typical lectins	959:981	Several typical lectins	959:981	Several typical lectins were chosen from the lectin microarray results.					
30074767	14	72	theme	easy	1608:1611	arg1	method					1624:1629	an easy and simple method	1605:1629	an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation	1605:1698	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	13	73	theme	DNA	1490:1492	arg1	load					1494:1497	the HBV viral DNA load	1476:1497	the HBV viral DNA load	1476:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	1	74	theme	B	125:125	arg1	HBV					134:136	HBV	134:136	HBV	134:136	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	74	theme	B	125:125	arg1	virus					164:168	a double-stranded DNA virus	142:168	a double-stranded DNA virus composed of three types of viral particles	142:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	1	74	theme	B	125:125	arg1	virus					127:131	Hepatitis B virus	115:131	Hepatitis B virus (HBV)	115:137	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	6	75	theme	HBsAg	579:583	arg1	glycosylation					585:597	HBsAg glycosylation	579:597	HBsAg glycosylation	579:597	HBsAg glycosylation is involved in viral particle formation and secretion.					
30074767	5	76	gly	glycoprotein	530:541	arg1	glycoprotein					530:541	glycoprotein	530:541	glycoprotein called HBV surface antigen (HBsAg)	530:576	Dane particles are covered with an envelope of glycoprotein called HBV surface antigen (HBsAg).					
30074767	13	77	theme	load	1494:1497	arg1	fraction					1464:1471	a major fraction	1456:1471	a major fraction of the HBV viral DNA load	1456:1497	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	13	78	theme	jacalin-bound	1423:1435	arg1	fraction					1437:1444	the jacalin-bound fraction	1419:1444	the jacalin-bound fraction	1419:1444	Interestingly, the jacalin-bound fraction contained a major fraction of the HBV viral DNA load.					
30074767	1	79	theme	viral	197:201	arg1	particles					203:211	viral particles	197:211	viral particles	197:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
30074767	0	80	theme	Particles	55:63	arg1	Profiling					24:32	Highly Sensitive Glycan Profiling	0:32	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles	0:63	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	0	80	theme	Particles	55:63	arg1	Method					78:83	a Simple Method	69:83	a Simple Method for Dane Particle Enrichment	69:112	Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.					
30074767	14	81	from	method	1557:1562	arg1	particle					1592:1599	the intact HBV particle	1577:1599	the intact HBV particle	1577:1599	Thus, in this study we have presented a glycan profiling method for HBsAg on the intact HBV particle and an easy and simple method to enrich Dane particles from patient serum by jacalin fractionation.					
30074767	11	82	theme	patient	1307:1313	arg1	serum					1315:1319	patient serum	1307:1319	patient serum	1307:1319	Employing the lectin-fractionation method using jacalin, HBV particles were fractionated into jacalin-bound and unbound fractions from patient serum.					
30074767	1	83	theme	particles	203:211	arg1	types					188:192	three types	182:192	three types of viral particles	182:211	Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles.					
29959030	9	0	theme	major	1393:1397	arg1	sorting					1380:1386	glycan-based protein sorting	1359:1386	glycan-based protein sorting	1359:1386	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	9	0	theme	major	1393:1397	arg1	mechanism					1399:1407	a major mechanism	1391:1407	a major mechanism for protein incorporation into ectosomes	1391:1448	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	4	1	theme	membrane	571:578	arg1	fractions					580:588	membrane fractions	571:588	membrane fractions	571:588	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	8	2	contain	contain	1235:1241	arg2	signal					1312:1317	a specific sorting signal	1293:1317	a specific sorting signal	1293:1317	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	2	contain	contain	1235:1241	arg2	composition					1259:1269	a unique glycan composition	1243:1269	a unique glycan composition	1243:1269	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	2	contain	contain	1235:1241	arg1	SIGNIFICANCE					1113:1124	SIGNIFICANCE	1113:1124	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane	1113:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	4	3	theme	Western	632:638	arg1	Blot					640:643	Western Blot	632:643	Western Blot	632:643	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	7	4	theme	proteins	1082:1089	arg1	glycosylation					1056:1068	the glycosylation	1052:1068	the glycosylation of ectosome proteins released by CM cells	1052:1110	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	5	5	theme	fucose	788:793	arg1	N-glycans					812:820	fucose and complex type N-glycans	788:820	N-glycans	812:820	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	6	6	theme	lectin	948:953	arg1	patterns					963:970	similar lectin binding patterns	940:970	similar lectin binding patterns	940:970	Moreover, similar lectin binding patterns were observed between related cell lines.					
29959030	8	7	theme	glycan	1252:1257	arg1	composition					1259:1269	a unique glycan composition	1243:1269	a unique glycan composition	1243:1269	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	7	theme	glycan	1252:1257	arg1	signal					1312:1317	a specific sorting signal	1293:1317	a specific sorting signal	1293:1317	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	4	8	theme	lectins	618:624	arg1	panel					609:613	a panel	607:613	a panel of lectins using Western Blot and flow cytometry	607:662	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	5	9	theme	bisecting	827:835	arg1	GlcNAc					837:842	bisecting GlcNAc	827:842	bisecting GlcNAc	827:842	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	7	10	theme	first	1024:1028	arg1	It					1014:1015	It	1014:1015	It	1014:1015	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	7	10	theme	first	1024:1028	arg1	study					1030:1034	the first study	1020:1034	the first study to characterize the glycosylation of ectosome proteins released by CM cells	1020:1110	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	1	11	theme	extracellular	128:140	arg1	vesicles					142:149	extracellular vesicles	128:149	extracellular vesicles	128:149	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	4	12	theme	disease	689:695	arg1	stage					697:701	disease stage	689:701	disease stage	689:701	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	5	13	theme	parental	905:912	arg1	membranes					919:927	the parental cell membranes	901:927	the parental cell membranes	901:927	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	1	14	theme	vesicles	142:149	arg1	involvement					113:123	the involvement	109:123	the involvement of extracellular vesicles in cancer development and progression	109:187	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	8	15	theme	specific	1295:1302	arg1	composition					1259:1269	a unique glycan composition	1243:1269	a unique glycan composition	1243:1269	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	15	theme	specific	1295:1302	arg1	signal					1312:1317	a specific sorting signal	1293:1317	a specific sorting signal	1293:1317	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	5	16	theme	FINDINGS	727:734	arg1	proteins					746:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	5	17	theme	complex	799:805	arg1	N-glycans					812:820	fucose and complex type N-glycans	788:820	N-glycans	812:820	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	5	18	theme	specific	861:868	arg1	glycoepitopes					870:882	specific glycoepitopes	861:882	specific glycoepitopes	861:882	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	6	19	theme	related	994:1000	arg1	lines					1007:1011	related cell lines	994:1011	related cell lines	994:1011	Moreover, similar lectin binding patterns were observed between related cell lines.					
29959030	8	20	dep	findings	1159:1166	arg1	derive					1183:1188	derive	1183:1188	derive from particular regions of the cell membrane	1183:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	4	21	from	fractions	580:588	arg1	Proteins					518:525	Proteins	518:525	Proteins from ectosomes, the whole cell extracts and membrane fractions	518:588	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	9	22	theme	clinical	1519:1526	arg1	implications					1528:1539	possible clinical implications	1510:1539	possible clinical implications	1510:1539	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	3	23	theme	METHODS	354:360	arg1	Ectosomes					362:370	MAIN METHODS Ectosomes	349:370	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	349:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	3	24	dep	primary	384:390	arg1	WM1205Lu					432:439	WM1205Lu	432:439	WM1205Lu	432:439	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	3	24	dep	primary	384:390	arg1	WM266-4					423:429	WM266-4	423:429	WM266-4	423:429	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	3	24	dep	primary	384:390	arg1	WM793					400:404	WM793	400:404	WM793	400:404	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	2	25	with	lines	318:322	arg1	use					333:335	the use	329:335	the use of lectins	329:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	1	26	theme	Numerous	82:89	arg1	studies					91:97	AIMS Numerous studies	77:97	AIMS Numerous studies	77:97	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	3	27	theme	primary	384:390	arg1	cells					445:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	8	28	dep	SIGNIFICANCE	1113:1124	arg1	supports					1146:1153	supports	1146:1153	supports the findings that ectosomes derive from particular regions of the cell membrane	1146:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	0	29	theme	Human	0:4	arg1	ectosomes					23:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes are enriched with specific glycan epitopes.					
29959030	4	30	from	ectosomes	532:540	arg1	Proteins					518:525	Proteins	518:525	Proteins from ectosomes, the whole cell extracts and membrane fractions	518:588	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	2	31	theme	present	194:200	arg1	study					202:206	The present study	190:206	The present study	190:206	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	8	32	theme	membrane	1226:1233	arg1	regions					1206:1212	particular regions	1195:1212	particular regions of the cell membrane	1195:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	2	33	theme	lectins	340:346	arg1	use					333:335	the use	329:335	the use of lectins	329:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	0	34	theme	specific	51:58	arg1	epitopes					67:74	specific glycan epitopes	51:74	specific glycan epitopes	51:74	Human melanoma-derived ectosomes are enriched with specific glycan epitopes.					
29959030	2	35	theme	ectosomes	262:270	arg1	composition					247:257	the glycan composition	236:257	the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins	236:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	1	36	from	involvement	113:123	arg1	development					161:171	cancer development	154:171	cancer development	154:171	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	1	36	from	involvement	113:123	arg1	progression					177:187	progression	177:187	progression	177:187	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	9	37	theme	glycan-based	1359:1370	arg1	sorting					1380:1386	glycan-based protein sorting	1359:1386	glycan-based protein sorting	1359:1386	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	9	37	theme	glycan-based	1359:1370	arg1	mechanism					1399:1407	a major mechanism	1391:1407	a major mechanism for protein incorporation into ectosomes	1391:1448	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	2	38	theme	cutaneous	289:297	arg1	CM					309:310	CM	309:310	CM	309:310	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	2	38	theme	cutaneous	289:297	arg1	melanoma					299:306	cutaneous melanoma	289:306	human cutaneous melanoma (CM) cell lines with the use of lectins	283:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	7	39	theme	CM	1103:1104	arg1	cells					1106:1110	CM cells	1103:1110	CM cells	1103:1110	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	3	40	theme	conditioned	470:480	arg1	media					482:486	conditioned media	470:486	conditioned media	470:486	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	4	41	theme	cell	553:556	arg1	extracts					558:565	the whole cell extracts	543:565	the whole cell extracts	543:565	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	0	42	link	melanoma-derived	6:21	arg1	ectosomes					23:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes are enriched with specific glycan epitopes.					
29959030	9	43	theme	possible	1510:1517	arg1	implications					1528:1539	possible clinical implications	1510:1539	possible clinical implications	1510:1539	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	7	44	theme	ectosome	1073:1080	arg1	proteins					1082:1089	ectosome proteins	1073:1089	ectosome proteins released by CM cells	1073:1110	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	2	45	theme	melanoma	299:306	arg1	lines					318:322	human cutaneous melanoma (CM) cell lines	283:322	human cutaneous melanoma (CM) cell lines with the use of lectins	283:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	9	46	theme	protein	1413:1419	arg1	incorporation					1421:1433	protein incorporation	1413:1433	protein incorporation into ectosomes	1413:1448	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	5	47	with	enrichment	764:773	arg1	N-glycans					812:820	fucose and complex type N-glycans	788:820	N-glycans	812:820	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	8	48	theme	cell	1221:1224	arg1	membrane					1226:1233	the cell membrane	1217:1233	the cell membrane	1217:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	6	49	theme	binding	955:961	arg1	patterns					963:970	similar lectin binding patterns	940:970	similar lectin binding patterns	940:970	Moreover, similar lectin binding patterns were observed between related cell lines.					
29959030	7	50	gly	glycosylation	1056:1068	arg1	proteins					1082:1089	ectosome proteins	1073:1089	ectosome proteins released by CM cells	1073:1110	It is the first study to characterize the glycosylation of ectosome proteins released by CM cells.					
29959030	2	51	theme	cell	313:316	arg1	lines					318:322	human cutaneous melanoma (CM) cell lines	283:322	human cutaneous melanoma (CM) cell lines with the use of lectins	283:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	5	52	theme	type	807:810	arg1	N-glycans					812:820	fucose and complex type N-glycans	788:820	N-glycans	812:820	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	6	53	theme	similar	940:946	arg1	patterns					963:970	similar lectin binding patterns	940:970	similar lectin binding patterns	940:970	Moreover, similar lectin binding patterns were observed between related cell lines.					
29959030	8	54	theme	unique	1245:1250	arg1	composition					1259:1269	a unique glycan composition	1243:1269	a unique glycan composition	1243:1269	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	54	theme	unique	1245:1250	arg1	signal					1312:1317	a specific sorting signal	1293:1317	a specific sorting signal	1293:1317	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	9	55	theme	new	1462:1464	arg1	means					1466:1470	new means	1462:1470	new means to control vesicular content and have possible clinical implications	1462:1539	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	8	56	theme	particular	1195:1204	arg1	regions					1206:1212	particular regions	1195:1212	particular regions of the cell membrane	1195:1233	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	2	57	theme	glycan	240:245	arg1	composition					247:257	the glycan composition	236:257	the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins	236:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	2	58	theme	human	283:287	arg1	lines					318:322	human cutaneous melanoma (CM) cell lines	283:322	human cutaneous melanoma (CM) cell lines with the use of lectins	283:346	The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins.					
29959030	9	59	theme	vesicular	1483:1491	arg1	content					1493:1499	vesicular content	1483:1499	vesicular content	1483:1499	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	5	60	theme	KEY	723:725	arg1	proteins					746:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	5	61	from	depletion	848:856	arg1	comparison					887:896	comparison	887:896	comparison to the parental cell membranes	887:927	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	1	62	theme	cancer	154:159	arg1	development					161:171	cancer development	154:171	cancer development	154:171	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	6	63	theme	cell	1002:1005	arg1	lines					1007:1011	related cell lines	994:1011	related cell lines	994:1011	Moreover, similar lectin binding patterns were observed between related cell lines.					
29959030	4	64	theme	flow	649:652	arg1	cytometry					654:662	flow cytometry	649:662	flow cytometry	649:662	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	5	65	theme	Ectosomal	736:744	arg1	proteins					746:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins	723:753	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	5	66	theme	glycoepitopes	870:882	arg1	enrichment					764:773	enrichment	764:773	enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc)	764:843	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	5	66	theme	glycoepitopes	870:882	arg1	depletion					848:856	depletion	848:856	depletion of specific glycoepitopes in comparison to the parental cell membranes	848:927	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	8	67	theme	sorting	1304:1310	arg1	composition					1259:1269	a unique glycan composition	1243:1269	a unique glycan composition	1243:1269	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	8	67	theme	sorting	1304:1310	arg1	signal					1312:1317	a specific sorting signal	1293:1317	a specific sorting signal	1293:1317	SIGNIFICANCE Our data indirectly supports the findings that ectosomes derive from particular regions of the cell membrane contain a unique glycan composition, which could serve as a specific sorting signal.					
29959030	5	68	from	enrichment	764:773	arg1	comparison					887:896	comparison	887:896	comparison to the parental cell membranes	887:927	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	4	69	from	extracts	558:565	arg1	Proteins					518:525	Proteins	518:525	Proteins from ectosomes, the whole cell extracts and membrane fractions	518:588	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	1	70	theme	AIMS	77:80	arg1	studies					91:97	AIMS Numerous studies	77:97	AIMS Numerous studies	77:97	AIMS Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression.					
29959030	3	71	theme	MAIN	349:352	arg1	Ectosomes					362:370	MAIN METHODS Ectosomes	349:370	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	349:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	0	72	theme	melanoma-derived	6:21	arg1	ectosomes					23:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes	0:31	Human melanoma-derived ectosomes are enriched with specific glycan epitopes.					
29959030	5	73	theme	cell	914:917	arg1	membranes					919:927	the parental cell membranes	901:927	the parental cell membranes	901:927	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	4	74	theme	stage	697:701	arg1	terms					680:684	terms	680:684	terms of disease stage and glycosignature	680:720	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	3	75	theme	metastatic	411:420	arg1	cells					445:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	4	76	theme	whole	547:551	arg1	extracts					558:565	the whole cell extracts	543:565	the whole cell extracts	543:565	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	4	77	theme	glycosignature	707:720	arg1	terms					680:684	terms	680:684	terms of disease stage and glycosignature	680:720	Proteins from ectosomes, the whole cell extracts and membrane fractions were probed with a panel of lectins using Western Blot and flow cytometry and compared in terms of disease stage and glycosignature.					
29959030	3	78	theme	CM	442:443	arg1	cells					445:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	384:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	5	79	with	N-glycans	812:820	arg1	GlcNAc					837:842	bisecting GlcNAc	827:842	bisecting GlcNAc	827:842	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	0	80	theme	glycan	60:65	arg1	epitopes					67:74	specific glycan epitopes	51:74	specific glycan epitopes	51:74	Human melanoma-derived ectosomes are enriched with specific glycan epitopes.					
29959030	5	81	with	depletion	848:856	arg1	N-glycans					812:820	fucose and complex type N-glycans	788:820	N-glycans	812:820	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	9	82	theme	protein	1372:1378	arg1	sorting					1380:1386	glycan-based protein sorting	1359:1386	glycan-based protein sorting	1359:1386	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	9	82	theme	protein	1372:1378	arg1	mechanism					1399:1407	a major mechanism	1391:1407	a major mechanism for protein incorporation into ectosomes	1391:1448	If proven correct, the hypothesis that glycan-based protein sorting is a major mechanism for protein incorporation into ectosomes may provide new means to control vesicular content and have possible clinical implications.					
29959030	3	83	attach	isolated	456:463	arg2	Ectosomes					362:370	MAIN METHODS Ectosomes	349:370	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells	349:449	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	3	83	attach	isolated	456:463	arg1	media					482:486	conditioned media	470:486	conditioned media	470:486	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
29959030	5	84	dep	N-glycans	812:820	arg1	mainly					776:781	mainly	776:781	mainly	776:781	KEY FINDINGS Ectosomal proteins revealed enrichment (mainly with fucose and complex type N-glycans with bisecting GlcNAc) or depletion of specific glycoepitopes in comparison to the parental cell membranes.					
29959030	3	85	theme	sequential	491:500	arg1	centrifugation					502:515	sequential centrifugation	491:515	sequential centrifugation	491:515	MAIN METHODS Ectosomes released by primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cells were isolated from conditioned media by sequential centrifugation.					
30138037	12	0	theme	hybrid/complex	2213:2226	arg1	N-glycosylation					2228:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	6	1	theme	hybrid/complex	1140:1153	arg1	N-glycosylation					1155:1169	hybrid/complex N-glycosylation	1140:1169	hybrid/complex N-glycosylation	1140:1169	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	9	2	theme	L-type	1535:1540	arg1	Cav					1570:1572	Cav	1570:1572	Cav	1570:1572	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	2	theme	L-type	1535:1540	arg1	channel					1561:1567	L-type voltage-gated Ca2+ channel	1535:1567	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	12	3	from	changes	2188:2194	arg1	N-glycosylation					2228:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	1	4	theme	essential	99:107	arg1	modification					127:138	an essential posttranslational modification	96:138	an essential posttranslational modification that affects a myriad of physiologic processes	96:185	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	1	4	theme	essential	99:107	arg1	glycosylation					79:91	Protein glycosylation	71:91	Protein glycosylation	71:91	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	9	5	theme	Ca2+	1556:1559	arg1	Cav					1570:1572	Cav	1570:1572	Cav	1570:1572	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	5	theme	Ca2+	1556:1559	arg1	channel					1561:1567	L-type voltage-gated Ca2+ channel	1535:1567	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	12	6	theme	relevant	2179:2186	arg1	changes					2188:2194	clinically relevant changes	2168:2194	clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation	2168:2242	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	11	7	from	levels	1941:1946	arg1	function					1905:1912	aberrant cardiac function	1888:1912	aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease	1888:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	2	8	theme	cardiac	300:306	arg1	deficits					308:315	significant cardiac deficits	288:315	significant cardiac deficits	288:315	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	12	9	theme	cardiac	2138:2144	arg1	disease					2146:2152	cardiac disease	2138:2152	cardiac disease	2138:2152	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	12	10	theme	cardiomyocyte	2199:2211	arg1	N-glycosylation					2228:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	cardiomyocyte hybrid/complex N-glycosylation	2199:2242	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	10	11	theme	Mgat1KO	1703:1709	arg1	handling					1730:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Consistently, Mgat1KO cardiomyocyte Ca2+ handling is altered and contraction is dyssynchronous compared with controls.					
30138037	9	12	theme	membrane	1668:1675	arg1	potentials					1677:1686	more-depolarized membrane potentials	1651:1686	more-depolarized membrane potentials	1651:1686	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	13	theme	more-depolarized	1651:1666	arg1	potentials					1677:1686	more-depolarized membrane potentials	1651:1686	more-depolarized membrane potentials	1651:1686	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	11	14	theme	altered	1978:1984	arg1	glycosylation					1986:1998	altered glycosylation	1978:1998	altered glycosylation	1978:1998	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	5	15	theme	N-glycan	771:778	arg1	structures					780:789	hybrid and complex N-glycan structures	752:789	hybrid and complex N-glycan structures	752:789	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	10	16	theme	Ca2+	1725:1728	arg1	handling					1730:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Consistently, Mgat1KO cardiomyocyte Ca2+ handling is altered and contraction is dyssynchronous compared with controls.					
30138037	5	17	theme	hybrid/complex	950:963	arg1	N-glycosylation					965:979	hybrid/complex N-glycosylation	950:979	hybrid/complex N-glycosylation	950:979	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	3	18	theme	heart	327:331	arg1	diseases					333:340	Acquired heart diseases	318:340	Acquired heart diseases	318:340	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	12	19	theme	early	2276:2280	arg1	death.-Ednie					2282:2293	early death.-Ednie	2276:2293	early death.-Ednie	2276:2293	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	11	20	theme	cardiac	1897:1903	arg1	function					1905:1912	aberrant cardiac function	1888:1912	aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease	1888:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	5	21	from	Golgi	805:809	arg1	necessary					721:729	necessary	721:729	necessary	721:729	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	22	theme	glycosyltransferase	553:571	arg1	gene					573:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	22	theme	glycosyltransferase	553:571	arg1	N-acetylglucosaminyltransferase					617:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	9	23	theme	GlcNAcT1	1595:1602	arg1	target					1604:1609	a GlcNAcT1 target	1593:1609	a GlcNAcT1 target	1593:1609	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	23	theme	GlcNAcT1	1595:1602	arg1	subunit					1575:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit	1519:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	8	24	theme	systolic	1427:1434	arg1	dysfunction					1436:1446	systolic dysfunction	1427:1446	systolic dysfunction resembling human dilated cardiomyopathy (DCM)	1427:1492	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	3	25	from	importance	437:446	arg1	disease					465:471	human cardiac disease	451:471	human cardiac disease	451:471	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	5	26	theme	end-stage	855:863	arg1	cardiomyopathy					865:878	human end-stage cardiomyopathy	849:878	human end-stage cardiomyopathy	849:878	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	1	27	theme	processes	177:185	arg1	myriad					155:160	a myriad	153:160	a myriad of physiologic processes	153:185	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	6	28	theme	KO	1064:1065	arg1	mouse					1068:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse	1023:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease	1023:1202	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	3	29	theme	associated	352:361	arg1	factors					368:374	their associated risk factors	346:374	their associated risk factors	346:374	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	9	30	theme	Cav	1624:1626	arg1	activity					1628:1635	Mgat1KO Cav activity	1616:1635	Mgat1KO Cav activity	1616:1635	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	6	31	theme	knockout	1054:1061	arg1	mouse					1068:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse	1023:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease	1023:1202	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	8	32	theme	dilated	1465:1471	arg1	cardiomyopathy					1473:1486	human dilated cardiomyopathy	1459:1486	human dilated cardiomyopathy (DCM)	1459:1492	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	8	32	theme	dilated	1465:1471	arg1	DCM					1489:1491	DCM	1489:1491	DCM	1489:1491	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	7	33	theme	truncated	1296:1304	arg1	structures					1315:1324	predominately truncated N-glycan structures	1282:1324	predominately truncated N-glycan structures	1282:1324	Biochemical and glycomic analyses showed that Mgat1KO cardiomyocytes produce predominately truncated N-glycan structures.					
30138037	6	34	theme	cardiomyocyte-specific	1025:1046	arg1	mouse					1068:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse	1023:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease	1023:1202	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	5	35	theme	medial	798:803	arg1	Golgi					805:809	the medial Golgi	794:809	the medial Golgi	794:809	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	36	from	levels	839:844	arg1	cardiomyopathy					865:878	human end-stage cardiomyopathy	849:878	human end-stage cardiomyopathy	849:878	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	37	theme	β-1,2-	610:615	arg1	Mgat1					650:654	Mgat1	650:654	Mgat1	650:654	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	37	theme	β-1,2-	610:615	arg1	N-acetylglucosaminyltransferase					617:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	37	theme	β-1,2-	610:615	arg1	gene					573:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	12	38	theme	Reduced	2340:2346	arg1	N-glycosylation					2364:2378	Reduced myocyte complex N-glycosylation	2340:2378	Reduced myocyte complex N-glycosylation	2340:2378	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	0	39	theme	Reduced	0:6	arg1	N-glycosylation					24:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	1	40	theme	posttranslational	109:125	arg1	modification					127:138	an essential posttranslational modification	96:138	an essential posttranslational modification that affects a myriad of physiologic processes	96:185	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	1	40	theme	posttranslational	109:125	arg1	glycosylation					79:91	Protein glycosylation	71:91	Protein glycosylation	71:91	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	12	41	theme	complex	2356:2362	arg1	N-glycosylation					2364:2378	Reduced myocyte complex N-glycosylation	2340:2378	Reduced myocyte complex N-glycosylation	2340:2378	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	0	42	theme	complex	16:22	arg1	N-glycosylation					24:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	3	43	theme	human	451:455	arg1	disease					465:471	human cardiac disease	451:471	human cardiac disease	451:471	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	7	44	theme	Mgat1KO	1251:1257	arg1	cardiomyocytes					1259:1272	Mgat1KO cardiomyocytes	1251:1272	Mgat1KO cardiomyocytes	1251:1272	Biochemical and glycomic analyses showed that Mgat1KO cardiomyocytes produce predominately truncated N-glycan structures.					
30138037	7	45	theme	glycomic	1221:1228	arg1	analyses					1230:1237	Biochemical and glycomic analyses	1205:1237	Biochemical and glycomic analyses	1205:1237	Biochemical and glycomic analyses showed that Mgat1KO cardiomyocytes produce predominately truncated N-glycan structures.					
30138037	5	46	theme	reduced	831:837	arg1	levels					839:844	reduced levels	831:844	reduced levels in human end-stage cardiomyopathy	831:878	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	12	47	from	reductions	2106:2115	arg1	glycosylation					2120:2132	glycosylation	2120:2132	glycosylation	2120:2132	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	12	47	from	reductions	2106:2115	arg1	disease					2146:2152	cardiac disease	2138:2152	cardiac disease	2138:2152	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	6	48	theme	cardiac	1175:1181	arg1	function					1183:1190	cardiac function	1175:1190	cardiac function	1175:1190	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	11	49	theme	hybrid/complex	1842:1855	arg1	N-glycosylation					1857:1871	reduced hybrid/complex N-glycosylation	1834:1871	reduced hybrid/complex N-glycosylation	1834:1871	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	9	50	theme	cardiomyocyte	1521:1533	arg1	target					1604:1609	a GlcNAcT1 target	1593:1609	a GlcNAcT1 target	1593:1609	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	50	theme	cardiomyocyte	1521:1533	arg1	α2δ1					1584:1587	α2δ1	1584:1587	α2δ1	1584:1587	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	50	theme	cardiomyocyte	1521:1533	arg1	subunit					1575:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit	1519:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	2	51	from	defects	208:214	arg1	glycosylation					219:231	glycosylation	219:231	glycosylation	219:231	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	9	52	theme	voltage-gated	1542:1554	arg1	Cav					1570:1572	Cav	1570:1572	Cav	1570:1572	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	52	theme	voltage-gated	1542:1554	arg1	channel					1561:1567	L-type voltage-gated Ca2+ channel	1535:1567	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	53	theme	channel	1561:1567	arg1	target					1604:1609	a GlcNAcT1 target	1593:1609	a GlcNAcT1 target	1593:1609	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	53	theme	channel	1561:1567	arg1	α2δ1					1584:1587	α2δ1	1584:1587	α2δ1	1584:1587	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	9	53	theme	channel	1561:1567	arg1	subunit					1575:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit	1519:1581	a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1)	1519:1588	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	7	54	theme	Biochemical	1205:1215	arg1	analyses					1230:1237	Biochemical and glycomic analyses	1205:1237	Biochemical and glycomic analyses	1205:1237	Biochemical and glycomic analyses showed that Mgat1KO cardiomyocytes produce predominately truncated N-glycan structures.					
30138037	8	55	theme	Mgat1KO	1331:1337	arg1	mice					1339:1342	All Mgat1KO mice	1327:1342	All Mgat1KO mice	1327:1342	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	10	56	theme	cardiomyocyte	1711:1723	arg1	handling					1730:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Mgat1KO cardiomyocyte Ca2+ handling	1703:1737	Consistently, Mgat1KO cardiomyocyte Ca2+ handling is altered and contraction is dyssynchronous compared with controls.					
30138037	5	57	theme	attractive	902:911	arg1	target					913:918	an attractive target	899:918	an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis	899:1003	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	11	58	theme	direct	1958:1963	arg1	link					1965:1968	a direct link	1956:1968	a direct link between altered glycosylation and heart disease	1956:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	5	59	theme	hybrid	752:757	arg1	structures					780:789	hybrid and complex N-glycan structures	752:789	hybrid and complex N-glycan structures	752:789	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	3	60	theme	Acquired	318:325	arg1	diseases					333:340	Acquired heart diseases	318:340	Acquired heart diseases	318:340	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	5	61	theme	complex	763:769	arg1	N-glycan					771:778	complex N-glycan	763:778	complex N-glycan	763:778	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	12	62	theme	systemic	2097:2104	arg1	reductions					2106:2115	systemic reductions	2097:2115	systemic reductions in glycosylation and cardiac disease	2097:2152	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	8	63	theme	control	1376:1382	arg1	mice					1384:1387	control mice	1376:1387	control mice	1376:1387	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	2	64	theme	truncated	250:258	arg1	glycans					260:266	truncated glycans	250:266	truncated glycans	250:266	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	5	65	theme	α-1,3-	589:594	arg1	Mgat1					650:654	Mgat1	650:654	Mgat1	650:654	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	65	theme	α-1,3-	589:594	arg1	N-acetylglucosaminyltransferase					617:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	65	theme	α-1,3-	589:594	arg1	gene					573:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	2	66	theme	significant	288:298	arg1	deficits					308:315	significant cardiac deficits	288:315	significant cardiac deficits	288:315	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	5	67	theme	-glycoprotein	596:608	arg1	Mgat1					650:654	Mgat1	650:654	Mgat1	650:654	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	67	theme	-glycoprotein	596:608	arg1	N-acetylglucosaminyltransferase					617:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	67	theme	-glycoprotein	596:608	arg1	gene					573:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	68	theme	N-glycosylation	965:979	arg1	role					942:945	the role	938:945	the role of hybrid/complex N-glycosylation in cardiac pathogenesis	938:1003	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	8	69	theme	chamber	1406:1412	arg1	dilation					1414:1421	chamber dilation	1406:1421	chamber dilation	1406:1421	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	1	70	theme	physiologic	165:175	arg1	processes					177:185	physiologic processes	165:185	physiologic processes	165:185	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	11	71	theme	aberrant	1888:1895	arg1	function					1905:1912	aberrant cardiac function	1888:1912	aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease	1888:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	7	72	theme	N-glycan	1306:1313	arg1	structures					1315:1324	predominately truncated N-glycan structures	1282:1324	predominately truncated N-glycan structures	1282:1324	Biochemical and glycomic analyses showed that Mgat1KO cardiomyocytes produce predominately truncated N-glycan structures.					
30138037	5	73	theme	mannosyl	579:586	arg1	Mgat1					650:654	Mgat1	650:654	Mgat1	650:654	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	73	theme	mannosyl	579:586	arg1	N-acetylglucosaminyltransferase					617:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	73	theme	mannosyl	579:586	arg1	gene					573:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene	549:576	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	9	74	theme	Mgat1KO	1616:1622	arg1	activity					1628:1635	Mgat1KO Cav activity	1616:1635	Mgat1KO Cav activity	1616:1635	Data also indicate that a cardiomyocyte L-type voltage-gated Ca2+ channel (Cav) subunit (α2δ1) is a GlcNAcT1 target, and Mgat1KO Cav activity is shifted to more-depolarized membrane potentials.					
30138037	3	75	theme	risk	363:366	arg1	factors					368:374	their associated risk factors	346:374	their associated risk factors	346:374	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	3	76	attach	linked	386:391	arg2	diseases					333:340	Acquired heart diseases	318:340	Acquired heart diseases	318:340	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	3	76	attach	linked	386:391	arg1	glycosylation					405:417	aberrant glycosylation	396:417	aberrant glycosylation	396:417	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	3	76	attach	linked	386:391	arg2	factors					368:374	their associated risk factors	346:374	their associated risk factors	346:374	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	11	77	theme	myocyte	1933:1939	arg1	levels					1941:1946	whole-heart and myocyte levels	1917:1946	whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease	1917:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	5	78	theme	structures	780:789	arg1	formation					739:747	the formation	735:747	the formation of hybrid and complex N-glycan structures	735:789	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	79	from	role	942:945	arg1	pathogenesis					992:1003	cardiac pathogenesis	984:1003	cardiac pathogenesis	984:1003	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	1	80	theme	Protein	71:77	arg1	modification					127:138	an essential posttranslational modification	96:138	an essential posttranslational modification that affects a myriad of physiologic processes	96:185	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	1	80	theme	Protein	71:77	arg1	glycosylation					79:91	Protein glycosylation	71:91	Protein glycosylation	71:91	Protein glycosylation is an essential posttranslational modification that affects a myriad of physiologic processes.					
30138037	6	81	theme	Mgat1	1048:1052	arg1	mouse					1068:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse	1023:1072	a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease	1023:1202	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	3	82	theme	aberrant	396:403	arg1	glycosylation					405:417	aberrant glycosylation	396:417	aberrant glycosylation	396:417	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	8	83	theme	human	1459:1463	arg1	cardiomyopathy					1473:1486	human dilated cardiomyopathy	1459:1486	human dilated cardiomyopathy (DCM)	1459:1492	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	8	83	theme	human	1459:1463	arg1	DCM					1489:1491	DCM	1489:1491	DCM	1489:1491	All Mgat1KO mice died significantly younger than control mice and demonstrated chamber dilation and systolic dysfunction resembling human dilated cardiomyopathy (DCM).					
30138037	11	84	theme	whole-heart	1917:1927	arg1	levels					1941:1946	whole-heart and myocyte levels	1917:1946	whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease	1917:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	12	85	theme	dilated	2387:2393	arg1	cardiomyopathy					2395:2408	dilated cardiomyopathy	2387:2408	dilated cardiomyopathy	2387:2408	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	0	86	theme	myocyte	8:14	arg1	N-glycosylation					24:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation	0:38	Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	5	87	theme	cardiac	984:990	arg1	pathogenesis					992:1003	cardiac pathogenesis	984:1003	cardiac pathogenesis	984:1003	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	5	88	theme	human	849:853	arg1	cardiomyopathy					865:878	human end-stage cardiomyopathy	849:878	human end-stage cardiomyopathy	849:878	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	6	89	theme	useful	1095:1100	arg1	model					1089:1093	a model	1087:1093	a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease	1087:1202	Here, we created a cardiomyocyte-specific Mgat1 knockout (KO) mouse to establish a model useful in exploring the relationship between hybrid/complex N-glycosylation and cardiac function and disease.					
30138037	5	90	gly	-glycoprotein	596:608	arg1	-glycoprotein					596:608	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase	579:647	mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1)	579:655	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	12	91	theme	myocyte	2348:2354	arg1	N-glycosylation					2364:2378	Reduced myocyte complex N-glycosylation	2340:2378	Reduced myocyte complex N-glycosylation	2340:2378	Thus, the Mgat1KO provides a model for investigating the relationship between systemic reductions in glycosylation and cardiac disease, showing that clinically relevant changes in cardiomyocyte hybrid/complex N-glycosylation are sufficient to cause DCM and early death.-Ednie, A. R., Deng, W., Yip, K.-P., Bennett, E. S. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	0	92	theme	dilated	47:53	arg1	cardiomyopathy					55:68	dilated cardiomyopathy	47:68	dilated cardiomyopathy	47:68	Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.					
30138037	2	93	with	Humans	188:193	arg1	defects					208:214	genetic defects	200:214	genetic defects in glycosylation	200:231	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	3	94	theme	cardiac	457:463	arg1	disease					465:471	human cardiac disease	451:471	human cardiac disease	451:471	Acquired heart diseases and their associated risk factors were also linked to aberrant glycosylation, highlighting its importance in human cardiac disease.					
30138037	2	95	theme	genetic	200:206	arg1	defects					208:214	genetic defects	200:214	genetic defects in glycosylation	200:231	Humans with genetic defects in glycosylation, which result in truncated glycans, often present with significant cardiac deficits.					
30138037	5	96	from	necessary	721:729	arg1	Golgi					805:809	the medial Golgi	794:809	the medial Golgi	794:809	The glycosyltransferase gene, mannosyl (α-1,3-)-glycoprotein β-1,2- N-acetylglucosaminyltransferase (Mgat1), whose product, N-acetylglucosaminyltransferase 1 (GlcNAcT1) is necessary for the formation of hybrid and complex N-glycan structures in the medial Golgi, was shown to be at reduced levels in human end-stage cardiomyopathy, thus making Mgat1 an attractive target for investigating the role of hybrid/complex N-glycosylation in cardiac pathogenesis.					
30138037	11	97	theme	reduced	1834:1840	arg1	N-glycosylation					1857:1871	reduced hybrid/complex N-glycosylation	1834:1871	reduced hybrid/complex N-glycosylation	1834:1871	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
30138037	11	98	theme	heart	2004:2008	arg1	disease					2010:2016	heart disease	2004:2016	heart disease	2004:2016	The data demonstrate that reduced hybrid/complex N-glycosylation contributes to aberrant cardiac function at whole-heart and myocyte levels drawing a direct link between altered glycosylation and heart disease.					
29461547	2	0	from	analysis	382:389	arg1	surfaces					412:419	living cell surfaces	400:419	living cell surfaces	400:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	8	1	theme	proposed	1577:1584	arg1	methods					1586:1592	The proposed methods	1573:1592	The proposed methods	1573:1592	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	5	2	used	used	1156:1159	arg2	PCDA-Nap					1089:1096	the monomer PCDA-Nap	1077:1096	the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore	1077:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	2	3	theme	Effective	326:334	arg1	methods					336:342	Effective methods	326:342	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces	326:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	7	4	theme	strong	1425:1430	arg1	binding					1432:1438	the strong binding	1421:1438	the strong binding of SA with PBA moieties	1421:1462	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	9	5	theme	cell	1820:1823	arg1	membranes					1825:1833	living cell membranes	1813:1833	living cell membranes	1813:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	8	6	theme	SA	1696:1697	arg1	detection					1628:1636	the fluorescence turn-on detection	1603:1636	the fluorescence turn-on detection of free SA in aqueous solution	1603:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	8	6	theme	SA	1696:1697	arg1	imaging					1685:1691	the in situ imaging	1673:1691	the in situ imaging of SA	1673:1697	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	7	7	with	SA	1443:1444	arg1	moieties					1455:1462	PBA moieties	1451:1462	PBA moieties	1451:1462	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	5	8	theme	fluorescence	1165:1176	arg1	signaling					1178:1186	fluorescence signaling	1165:1186	fluorescence signaling	1165:1186	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	3	9	theme	turn-on	677:683	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	9	theme	turn-on	677:683	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	2	10	theme	living	400:405	arg1	surfaces					412:419	living cell surfaces	400:419	living cell surfaces	400:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	1	11	theme	located	162:168	arg1	acid					152:155	Sialic acid	145:155	Sialic acid (SA) located at the terminal end of glycans on cell membranes	145:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	1	11	theme	located	162:168	arg1	SA					158:159	SA	158:159	SA	158:159	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	7	12	theme	abundant	1405:1412	arg1	cells					1414:1418	SA abundant cells	1402:1418	SA abundant cells	1402:1418	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	9	13	from	analysis	1795:1802	arg1	membranes					1825:1833	living cell membranes	1813:1833	living cell membranes	1813:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	3	14	from	sensor	685:690	arg1	surfaces					805:812	living cell surfaces	793:812	living cell surfaces	793:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	15	theme	free	713:716	arg1	SA					718:719	free SA	713:719	free SA	713:719	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	6	16	theme	composite	1198:1206	arg1	liposomes					1212:1220	the composite PDA liposomes	1194:1220	the composite PDA liposomes	1194:1220	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	3	17	theme	PDA	527:529	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	17	theme	PDA	527:529	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	17	theme	PDA	527:529	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	6	18	dep	fluorophore	1267:1277	arg1	the					1263:1265	the	1263:1265	the	1263:1265	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	3	19	from	surfaces	805:812	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	19	from	surfaces	805:812	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	19	from	surfaces	805:812	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	0	20	theme	cell-surface	116:127	arg1	imaging					136:142	cell-surface glycan imaging	116:142	cell-surface glycan imaging	116:142	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	5	21	theme	PBA	1010:1012	arg1	molecules					1014:1022	PBA molecules	1010:1022	PBA molecules	1010:1022	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	3	22	theme	polydiacetylene	510:524	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	22	theme	polydiacetylene	510:524	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	22	theme	polydiacetylene	510:524	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	2	23	from	significance	434:445	arg1	terms					450:454	terms	450:454	terms of clinical diagnostics and therapeutics	450:495	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	1	24	theme	glycans	193:199	arg1	end					186:188	the terminal end	173:188	the terminal end of glycans on cell membranes	173:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	5	25	contain	containing	1098:1107	arg1	PCDA-Nap					1089:1096	the monomer PCDA-Nap	1077:1096	the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore	1077:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	5	25	contain	containing	1098:1107	arg2	fluorophore					1140:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	1	26	theme	important	245:253	arg1	role					271:274	an important yet distinctive role	242:274	an important yet distinctive role	242:274	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	5	27	theme	monomer	979:985	arg1	receptor					1042:1049	a receptor	1040:1049	a receptor for SA recognition	1040:1068	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	5	27	theme	monomer	979:985	arg1	PCDA-pBA					987:994	The monomer PCDA-pBA	975:994	The monomer PCDA-pBA modified with PBA molecules	975:1022	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	3	28	theme	liposome-based	532:545	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	28	theme	liposome-based	532:545	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	28	theme	liposome-based	532:545	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	8	29	theme	MCF-7	1709:1713	arg1	surfaces					1720:1727	living MCF-7 cell surfaces	1702:1727	living MCF-7 cell surfaces	1702:1727	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	8	30	theme	living	1702:1707	arg1	surfaces					1720:1727	living MCF-7 cell surfaces	1702:1727	living MCF-7 cell surfaces	1702:1727	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	3	31	theme	living	793:798	arg1	surfaces					805:812	living cell surfaces	793:812	living cell surfaces	793:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	0	32	theme	fluorescence	58:69	arg1	sensor					79:84	a fluorescence turn-on sensor	56:84	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.	0:143	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	5	33	theme	SA	1055:1056	arg1	recognition					1058:1068	SA recognition	1055:1068	SA recognition	1055:1068	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	3	34	theme	phenylboronic	569:581	arg1	PBA					589:591	PBA	589:591	PBA	589:591	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	34	theme	phenylboronic	569:581	arg1	acid					583:586	phenylboronic acid	569:586	phenylboronic acid (PBA)	569:592	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	35	theme	glycans	782:788	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	35	theme	glycans	782:788	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	35	theme	glycans	782:788	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	2	36	theme	therapeutics	484:495	arg1	terms					450:454	terms	450:454	terms of clinical diagnostics and therapeutics	450:495	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	8	37	from	detection	1628:1636	arg1	solution					1660:1667	aqueous solution	1652:1667	aqueous solution	1652:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	0	38	theme	sialic	90:95	arg1	detection					102:110	sialic acid detection	90:110	sialic acid detection	90:110	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	2	39	theme	diagnostics	468:478	arg1	terms					450:454	terms	450:454	terms of clinical diagnostics and therapeutics	450:495	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	3	40	from	detection	700:708	arg1	solution					732:739	aqueous solution	724:739	aqueous solution	724:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	8	41	dep	in	1677:1678	arg1	situ					1680:1683	situ	1680:1683	situ	1680:1683	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	9	42	theme	potential	1755:1763	arg1	tool					1765:1768	a new potential tool	1749:1768	a new potential tool for simple and selective analysis of SA on living cell membranes	1749:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	2	43	theme	cell	407:410	arg1	surfaces					412:419	living cell surfaces	400:419	living cell surfaces	400:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	0	44	theme	turn-on	71:77	arg1	sensor					79:84	a fluorescence turn-on sensor	56:84	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.	0:143	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	0	45	theme	Polydiacetylene	0:14	arg1	liposomes					16:24	Polydiacetylene liposomes	0:24	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.	0:143	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	5	46	theme	derivative	1129:1138	arg1	fluorophore					1140:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	7	47	theme	pendent	1478:1484	arg1	conformation					1497:1508	the pendent side chain conformation	1474:1508	the pendent side chain conformation	1474:1508	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	2	48	theme	great	428:432	arg1	significance					434:445	great significance	428:445	great significance	428:445	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	3	49	from	glycans	782:788	arg1	surfaces					805:812	living cell surfaces	793:812	living cell surfaces	793:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	50	theme	1,8-naphthalimide	598:614	arg1	moieties					636:643	1,8-naphthalimide derived fluorophore moieties	598:643	1,8-naphthalimide derived fluorophore moieties	598:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	0	51	theme	acid	45:48	arg1	tags					50:53	phenylboronic acid tags	31:53	phenylboronic acid tags	31:53	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	4	52	theme	PDA	954:956	arg1	sensor					967:972	the composite PDA liposome sensor	940:972	the composite PDA liposome sensor	940:972	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	8	53	from	solution	1660:1667	arg1	detection					1628:1636	the fluorescence turn-on detection	1603:1636	the fluorescence turn-on detection of free SA in aqueous solution	1603:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	8	53	from	solution	1660:1667	arg1	imaging					1685:1691	the in situ imaging	1673:1691	the in situ imaging of SA	1673:1697	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	1	54	theme	various	279:285	arg1	processes					315:323	various biological and pathological processes	279:323	various biological and pathological processes	279:323	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	7	55	theme	PBA	1451:1453	arg1	moieties					1455:1462	PBA moieties	1451:1462	PBA moieties	1451:1462	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	3	56	link	derived	616:622	arg1	moieties					636:643	1,8-naphthalimide derived fluorophore moieties	598:643	1,8-naphthalimide derived fluorophore moieties	598:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	7	57	theme	SA	1443:1444	arg1	binding					1432:1438	the strong binding	1421:1438	the strong binding of SA with PBA moieties	1421:1462	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	3	58	theme	fluorophore	624:634	arg1	moieties					636:643	1,8-naphthalimide derived fluorophore moieties	598:643	1,8-naphthalimide derived fluorophore moieties	598:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	8	59	theme	turn-on	1620:1626	arg1	detection					1628:1636	the fluorescence turn-on detection	1603:1636	the fluorescence turn-on detection of free SA in aqueous solution	1603:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	9	60	theme	SA	1807:1808	arg1	analysis					1795:1802	simple and selective analysis	1774:1802	simple and selective analysis of SA on living cell membranes	1774:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	3	61	theme	fluorescence	664:675	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	61	theme	fluorescence	664:675	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	6	62	theme	energy	1239:1244	arg1	transfer					1246:1253	the energy transfer	1235:1253	the energy transfer between the fluorophore and the conjugated backbone	1235:1305	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	9	63	theme	living	1813:1818	arg1	membranes					1825:1833	living cell membranes	1813:1833	living cell membranes	1813:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	8	64	theme	SA	1646:1647	arg1	detection					1628:1636	the fluorescence turn-on detection	1603:1636	the fluorescence turn-on detection of free SA in aqueous solution	1603:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	8	64	theme	SA	1646:1647	arg1	imaging					1685:1691	the in situ imaging	1673:1691	the in situ imaging of SA	1673:1697	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	4	65	theme	sensor	822:827	arg1	system					829:834	the sensor system	818:834	the sensor system	818:834	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	8	66	theme	aqueous	1652:1658	arg1	solution					1660:1667	aqueous solution	1652:1667	aqueous solution	1652:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	9	67	theme	simple	1774:1779	arg1	analysis					1795:1802	simple and selective analysis	1774:1802	simple and selective analysis of SA on living cell membranes	1774:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	7	68	theme	SA	1402:1403	arg1	cells					1414:1418	SA abundant cells	1402:1418	SA abundant cells	1402:1418	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	7	69	theme	additional	1385:1394	arg1	SA					1396:1397	additional SA	1385:1397	additional SA	1385:1397	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	6	70	theme	PDA	1208:1210	arg1	liposomes					1212:1220	the composite PDA liposomes	1194:1220	the composite PDA liposomes	1194:1220	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	3	71	theme	SA	718:719	arg1	detection					700:708	the detection	696:708	the detection of free SA in aqueous solution	696:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	0	72	theme	glycan	129:134	arg1	imaging					136:142	cell-surface glycan imaging	116:142	cell-surface glycan imaging	116:142	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	3	73	theme	new	506:508	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	73	theme	new	506:508	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	73	theme	new	506:508	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	8	74	theme	in	1677:1678	arg1	imaging					1685:1691	the in situ imaging	1673:1691	the in situ imaging of SA	1673:1697	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	1	75	theme	terminal	177:184	arg1	end					186:188	the terminal end	173:188	the terminal end of glycans on cell membranes	173:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	3	76	dep	in	749:750	arg1	situ					752:755	situ	752:755	situ	752:755	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	77	theme	sensor	547:552	arg1	sensor					685:690	a fluorescence turn-on sensor	662:690	a fluorescence turn-on sensor for the detection of free SA in aqueous solution	662:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	77	theme	sensor	547:552	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	77	theme	sensor	547:552	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	7	78	theme	fluorophore	1560:1570	arg1	restoration					1541:1551	the fluorescence restoration	1524:1551	the fluorescence restoration of the fluorophore	1524:1570	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	9	79	theme	new	1751:1753	arg1	tool					1765:1768	a new potential tool	1749:1768	a new potential tool for simple and selective analysis of SA on living cell membranes	1749:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	1	80	from	membranes	209:217	arg1	end					186:188	the terminal end	173:188	the terminal end of glycans on cell membranes	173:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	1	81	from	glycans	193:199	arg1	membranes					209:217	cell membranes	204:217	cell membranes	204:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	3	82	theme	aqueous	724:730	arg1	solution					732:739	aqueous solution	724:739	aqueous solution	724:739	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	1	83	from	end	186:188	arg1	membranes					209:217	cell membranes	204:217	cell membranes	204:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	1	83	from	end	186:188	arg1	located					162:168	located	162:168	located	162:168	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	7	84	dep	SA	1396:1397	arg1	the					1369:1371	the	1369:1371	the	1369:1371	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	7	84	dep	SA	1396:1397	arg1	presence					1373:1380	presence	1373:1380	presence	1373:1380	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	7	85	theme	fluorescence	1528:1539	arg1	restoration					1541:1551	the fluorescence restoration	1524:1551	the fluorescence restoration of the fluorophore	1524:1570	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	4	86	theme	diacetylene	843:853	arg1	PCDA-pBA					865:872	PCDA-pBA	865:872	PCDA-pBA	865:872	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	4	86	theme	diacetylene	843:853	arg1	monomers					855:862	three diacetylene monomers	837:862	three diacetylene monomers	837:862	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	4	86	theme	diacetylene	843:853	arg1	PCDA-EA					888:894	PCDA-EA	888:894	PCDA-EA	888:894	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	4	86	theme	diacetylene	843:853	arg1	PCDA-Nap					875:882	PCDA-Nap	875:882	PCDA-Nap	875:882	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	6	87	theme	fluorophore	1353:1363	arg1	fluorescence					1333:1344	the fluorescence	1329:1344	the fluorescence of the fluorophore	1329:1363	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	8	88	theme	cell	1715:1718	arg1	surfaces					1720:1727	living MCF-7 cell surfaces	1702:1727	living MCF-7 cell surfaces	1702:1727	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	1	89	theme	Sialic	145:150	arg1	acid					152:155	Sialic acid	145:155	Sialic acid (SA) located at the terminal end of glycans on cell membranes	145:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	1	89	theme	Sialic	145:150	arg1	SA					158:159	SA	158:159	SA	158:159	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	3	90	theme	cell	800:803	arg1	surfaces					805:812	living cell surfaces	793:812	living cell surfaces	793:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	91	theme	in	749:750	arg1	system					554:559	a new polydiacetylene (PDA) liposome-based sensor system	504:559	a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties	504:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	91	theme	in	749:750	arg1	imaging					757:763	the in situ imaging	745:763	the in situ imaging of SA-terminated glycans on living cell surfaces	745:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	1	92	theme	pathological	302:313	arg1	processes					315:323	various biological and pathological processes	279:323	various biological and pathological processes	279:323	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	6	93	theme	conjugated	1287:1296	arg1	backbone					1298:1305	the conjugated backbone	1283:1305	the conjugated backbone	1283:1305	When the composite PDA liposomes were formed, the energy transfer between the fluorophore and the conjugated backbone could directly quench the fluorescence of the fluorophore.					
29461547	0	94	theme	acid	97:100	arg1	detection					102:110	sialic acid detection	90:110	sialic acid detection	90:110	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	7	95	theme	chain	1491:1495	arg1	conformation					1497:1508	the pendent side chain conformation	1474:1508	the pendent side chain conformation	1474:1508	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	3	96	theme	SA-terminated	768:780	arg1	glycans					782:788	SA-terminated glycans	768:788	SA-terminated glycans on living cell surfaces	768:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	2	97	theme	clinical	459:466	arg1	diagnostics					468:478	clinical diagnostics	459:478	clinical diagnostics	459:478	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	2	98	theme	sensitive	360:368	arg1	analysis					382:389	the facile, sensitive and in situ analysis	348:389	the facile, sensitive and in situ analysis of SA on living cell surfaces	348:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	4	99	theme	liposome	958:965	arg1	sensor					967:972	the composite PDA liposome sensor	940:972	the composite PDA liposome sensor	940:972	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	8	100	from	SA	1646:1647	arg1	solution					1660:1667	aqueous solution	1652:1667	aqueous solution	1652:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	2	101	theme	facile	352:357	arg1	analysis					382:389	the facile, sensitive and in situ analysis	348:389	the facile, sensitive and in situ analysis of SA on living cell surfaces	348:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	0	102	theme	phenylboronic	31:43	arg1	tags					50:53	phenylboronic acid tags	31:53	phenylboronic acid tags	31:53	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	5	103	theme	1,8-naphthalimide	1111:1127	arg1	fluorophore					1140:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	a 1,8-naphthalimide derivative fluorophore	1109:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	2	104	theme	SA	394:395	arg1	analysis					382:389	the facile, sensitive and in situ analysis	348:389	the facile, sensitive and in situ analysis of SA on living cell surfaces	348:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	1	105	theme	distinctive	259:269	arg1	role					271:274	an important yet distinctive role	242:274	an important yet distinctive role	242:274	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	7	106	theme	side	1486:1489	arg1	conformation					1497:1508	the pendent side chain conformation	1474:1508	the pendent side chain conformation	1474:1508	In the presence of additional SA or SA abundant cells, the strong binding of SA with PBA moieties disturbed the pendent side chain conformation, resulting in the fluorescence restoration of the fluorophore.					
29461547	2	107	dep	in	374:375	arg1	situ					377:380	situ	377:380	situ	377:380	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	2	108	theme	in	374:375	arg1	analysis					382:389	the facile, sensitive and in situ analysis	348:389	the facile, sensitive and in situ analysis of SA on living cell surfaces	348:419	Effective methods for the facile, sensitive and in situ analysis of SA on living cell surfaces are of great significance in terms of clinical diagnostics and therapeutics.					
29461547	5	109	theme	monomer	1081:1087	arg1	PCDA-Nap					1089:1096	the monomer PCDA-Nap	1077:1096	the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore	1077:1150	The monomer PCDA-pBA modified with PBA molecules was employed as a receptor for SA recognition, while the monomer PCDA-Nap containing a 1,8-naphthalimide derivative fluorophore was used for fluorescence signaling.					
29461547	4	110	theme	composite	944:952	arg1	sensor					967:972	the composite PDA liposome sensor	940:972	the composite PDA liposome sensor	940:972	In the sensor system, three diacetylene monomers, PCDA-pBA, PCDA-Nap and PCDA-EA, were designed and synthesized to construct the composite PDA liposome sensor.					
29461547	0	111	with	liposomes	16:24	arg1	tags					50:53	phenylboronic acid tags	31:53	phenylboronic acid tags	31:53	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	8	112	theme	fluorescence	1607:1618	arg1	detection					1628:1636	the fluorescence turn-on detection	1603:1636	the fluorescence turn-on detection of free SA in aqueous solution	1603:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	1	113	theme	biological	287:296	arg1	processes					315:323	various biological and pathological processes	279:323	various biological and pathological processes	279:323	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	9	114	theme	selective	1785:1793	arg1	analysis					1795:1802	simple and selective analysis	1774:1802	simple and selective analysis of SA on living cell membranes	1774:1833	This work provides a new potential tool for simple and selective analysis of SA on living cell membranes.					
29461547	3	115	from	imaging	757:763	arg1	surfaces					805:812	living cell surfaces	793:812	living cell surfaces	793:812	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	3	116	theme	derived	616:622	arg1	moieties					636:643	1,8-naphthalimide derived fluorophore moieties	598:643	1,8-naphthalimide derived fluorophore moieties	598:643	Here, a new polydiacetylene (PDA) liposome-based sensor system bearing phenylboronic acid (PBA) and 1,8-naphthalimide derived fluorophore moieties was developed as a fluorescence turn-on sensor for the detection of free SA in aqueous solution and the in situ imaging of SA-terminated glycans on living cell surfaces.					
29461547	8	117	from	imaging	1685:1691	arg1	solution					1660:1667	aqueous solution	1652:1667	aqueous solution	1652:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29461547	1	118	theme	cell	204:207	arg1	membranes					209:217	cell membranes	204:217	cell membranes	204:217	Sialic acid (SA) located at the terminal end of glycans on cell membranes has been shown to play an important yet distinctive role in various biological and pathological processes.					
29461547	0	119	dep	liposomes	16:24	arg1	sensor					79:84	a fluorescence turn-on sensor	56:84	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.	0:143	Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging.					
29461547	8	120	theme	free	1641:1644	arg1	SA					1646:1647	free SA	1641:1647	free SA in aqueous solution	1641:1667	The proposed methods realized the fluorescence turn-on detection of free SA in aqueous solution and the in situ imaging of SA on living MCF-7 cell surfaces.					
29949794	3	0	theme	glycosaminoglycan	357:373	arg1	biosynthesis					375:386	glycosaminoglycan biosynthesis	357:386	glycosaminoglycan biosynthesis	357:386	However, other studies indicate that genistein does not always inhibit glycosaminoglycan biosynthesis.					
29949794	8	1	theme	type-dependent	1272:1285	arg1	manner					1287:1292	a cell type-dependent manner	1265:1292	a cell type-dependent manner	1265:1292	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	5	2	theme	glycosaminoglycan	690:706	arg1	compositions					721:732	the glycosaminoglycan disaccharide compositions	686:732	the glycosaminoglycan disaccharide compositions	686:732	In addition, the glycosaminoglycan disaccharide compositions were obtained by stable isotope labeling coupled with LC/MS analysis.					
29949794	7	3	theme	glycan	972:977	arg1	quantities					1001:1010	both glycan and glycosaminoglycan quantities	967:1010	quantities	1001:1010	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	4	4	theme	acid	630:633	arg1	hydrolysis					635:644	thorough acid hydrolysis	621:644	thorough acid hydrolysis followed by HPLC analysis	621:670	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	8	5	theme	cell	1267:1270	arg1	manner					1287:1292	a cell type-dependent manner	1265:1292	a cell type-dependent manner	1265:1292	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	5	6	theme	disaccharide	708:719	arg1	compositions					721:732	the glycosaminoglycan disaccharide compositions	686:732	the glycosaminoglycan disaccharide compositions	686:732	In addition, the glycosaminoglycan disaccharide compositions were obtained by stable isotope labeling coupled with LC/MS analysis.					
29949794	6	7	theme	glycosaminoglycans	890:907	arg1	amount					880:885	the amount	876:885	the amount of glycosaminoglycans in HCT116 cells	876:923	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	6	7	theme	glycosaminoglycans	890:907	arg1	glycosaminoglycans					890:907	glycosaminoglycans	890:907	glycosaminoglycans	890:907	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	2	8	contain	has	164:166	arg1	It					161:162	It	161:162	It	161:162	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	2	8	contain	has	164:166	arg2	effects					179:185	beneficial effects	168:185	beneficial effects for patients with mucopolysaccharidose type III	168:233	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	4	9	theme	thorough	621:628	arg1	hydrolysis					635:644	thorough acid hydrolysis	621:644	thorough acid hydrolysis followed by HPLC analysis	621:670	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	4	10	from	quantity	556:563	arg1	lysate					594:599	the cell lysate	585:599	the cell lysate	585:599	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	7	11	theme	CHOK1	1015:1019	arg1	CHO3.1					1022:1027	CHOK1, CHO3.1, and CHO3.3 cells	1015:1045	CHO3.1	1022:1027	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	7	12	theme	differential	1062:1073	arg1	changes					1075:1081	differential changes	1062:1081	differential changes in glycosaminoglycan disaccharide compositions	1062:1128	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	8	13	theme	Genistein	1142:1150	arg1	treatment					1152:1160	CONCLUSION Genistein treatment	1131:1160	CONCLUSION Genistein treatment	1131:1160	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	4	14	from	compositions	539:550	arg1	lysate					594:599	the cell lysate	585:599	the cell lysate	585:599	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	7	15	theme	CHO3.3	1034:1039	arg1	cells					1041:1045	CHOK1, CHO3.1, and CHO3.3 cells	1015:1045	cells	1041:1045	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	7	16	theme	genistein	939:947	arg1	treatment					949:957	genistein treatment	939:957	genistein treatment	939:957	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	6	17	from	amount	880:885	arg1	cells					919:923	HCT116 cells	912:923	HCT116 cells	912:923	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	3	18	theme	other	295:299	arg1	studies					301:307	other studies	295:307	other studies	295:307	However, other studies indicate that genistein does not always inhibit glycosaminoglycan biosynthesis.					
29949794	4	19	theme	monosaccharide	524:537	arg1	compositions					539:550	the monosaccharide compositions	520:550	the monosaccharide compositions	520:550	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	4	20	theme	molecular	426:434	arg1	mechanisms					436:445	the underlying molecular mechanisms	411:445	the underlying molecular mechanisms	411:445	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	1	21	theme	BACKGROUND/AIMS	89:103	arg1	Genistein					105:113	BACKGROUND/AIMS Genistein	89:113	BACKGROUND/AIMS Genistein	89:113	BACKGROUND/AIMS Genistein is a natural isoflavone enriched in soybeans.					
29949794	1	21	theme	BACKGROUND/AIMS	89:103	arg1	isoflavone					128:137	a natural isoflavone	118:137	a natural isoflavone enriched in soybeans	118:158	BACKGROUND/AIMS Genistein is a natural isoflavone enriched in soybeans.					
29949794	8	22	theme	glycan	1178:1183	arg1	quantity					1185:1192	overall glycan quantity	1170:1192	overall glycan quantity	1170:1192	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	4	23	theme	CHOK1	448:452	arg1	CHO3.3					463:468	CHOK1, CHO3.1, CHO3.3, and HCT116 cells	448:486	CHO3.3	463:468	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	8	24	theme	overall	1170:1176	arg1	quantity					1185:1192	overall glycan quantity	1170:1192	overall glycan quantity	1170:1192	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	7	25	from	changes	1075:1081	arg1	compositions					1117:1128	glycosaminoglycan disaccharide compositions	1086:1128	glycosaminoglycan disaccharide compositions	1086:1128	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	6	26	theme	glycans	854:860	arg1	glycans					854:860	glycans	854:860	glycans	854:860	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	6	26	theme	glycans	854:860	arg1	amount					844:849	the amount	840:849	the amount of glycans	840:860	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	8	27	theme	glycosaminoglycan	1198:1214	arg1	quantities					1216:1225	glycosaminoglycan quantities	1198:1225	glycosaminoglycan quantities	1198:1225	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	7	28	from	quantities	1001:1010	arg1	CHO3.1					1022:1027	CHOK1, CHO3.1, and CHO3.3 cells	1015:1045	CHO3.1	1022:1027	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	7	28	from	quantities	1001:1010	arg1	cells					1041:1045	CHOK1, CHO3.1, and CHO3.3 cells	1015:1045	cells	1041:1045	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	4	29	from	lysate	594:599	arg1	quantity					556:563	quantity	556:563	quantity	556:563	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	4	29	from	lysate	594:599	arg1	glycans					572:578	all glycans	568:578	all glycans from the cell lysate	568:599	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	4	29	from	lysate	594:599	arg1	compositions					539:550	the monosaccharide compositions	520:550	the monosaccharide compositions	520:550	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	0	30	theme	Glycosaminoglycan	30:46	arg1	Quantity					48:55	Glycosaminoglycan Quantity	30:55	Glycosaminoglycan Quantity	30:55	Genistein Enhances or Reduces Glycosaminoglycan Quantity in a Cell Type-Specific Manner.					
29949794	5	31	theme	LC/MS	788:792	arg1	analysis					794:801	LC/MS analysis	788:801	LC/MS analysis	788:801	In addition, the glycosaminoglycan disaccharide compositions were obtained by stable isotope labeling coupled with LC/MS analysis.					
29949794	2	32	theme	glycosaminoglycan	254:270	arg1	biosynthesis					272:283	glycosaminoglycan biosynthesis	254:283	glycosaminoglycan biosynthesis	254:283	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	7	33	theme	disaccharide	1104:1115	arg1	compositions					1117:1128	glycosaminoglycan disaccharide compositions	1086:1128	glycosaminoglycan disaccharide compositions	1086:1128	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	6	34	theme	RESULTS	804:810	arg1	treatment					822:830	RESULTS Genistein treatment	804:830	RESULTS Genistein treatment	804:830	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	4	35	theme	glycans	572:578	arg1	quantity					556:563	quantity	556:563	quantity	556:563	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	4	35	theme	glycans	572:578	arg1	compositions					539:550	the monosaccharide compositions	520:550	the monosaccharide compositions	520:550	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	2	36	theme	beneficial	168:177	arg1	effects					179:185	beneficial effects	168:185	beneficial effects for patients with mucopolysaccharidose type III	168:233	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	1	37	theme	natural	120:126	arg1	Genistein					105:113	BACKGROUND/AIMS Genistein	89:113	BACKGROUND/AIMS Genistein	89:113	BACKGROUND/AIMS Genistein is a natural isoflavone enriched in soybeans.					
29949794	1	37	theme	natural	120:126	arg1	isoflavone					128:137	a natural isoflavone	118:137	a natural isoflavone enriched in soybeans	118:158	BACKGROUND/AIMS Genistein is a natural isoflavone enriched in soybeans.					
29949794	4	38	theme	cell	589:592	arg1	lysate					594:599	the cell lysate	585:599	the cell lysate	585:599	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	8	39	theme	CONCLUSION	1131:1140	arg1	treatment					1152:1160	CONCLUSION Genistein treatment	1131:1160	CONCLUSION Genistein treatment	1131:1160	CONCLUSION Genistein treatment reduced overall glycan quantity but glycosaminoglycan quantities were either increased or decreased in a cell type-dependent manner.					
29949794	4	40	theme	underlying	415:424	arg1	mechanisms					436:445	the underlying molecular mechanisms	411:445	the underlying molecular mechanisms	411:445	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	5	41	theme	stable	751:756	arg1	labeling					766:773	stable isotope labeling	751:773	stable isotope labeling coupled with LC/MS analysis	751:801	In addition, the glycosaminoglycan disaccharide compositions were obtained by stable isotope labeling coupled with LC/MS analysis.					
29949794	7	42	theme	glycosaminoglycan	1086:1102	arg1	compositions					1117:1128	glycosaminoglycan disaccharide compositions	1086:1128	glycosaminoglycan disaccharide compositions	1086:1128	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	2	43	with	patients	191:198	arg1	type					226:229	mucopolysaccharidose type III	205:233	mucopolysaccharidose type III	205:233	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	4	44	theme	HPLC	658:661	arg1	analysis					663:670	HPLC analysis	658:670	HPLC analysis	658:670	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	5	45	theme	isotope	758:764	arg1	labeling					766:773	stable isotope labeling	751:773	stable isotope labeling coupled with LC/MS analysis	751:801	In addition, the glycosaminoglycan disaccharide compositions were obtained by stable isotope labeling coupled with LC/MS analysis.					
29949794	2	46	theme	mucopolysaccharidose	205:224	arg1	type					226:229	mucopolysaccharidose type III	205:233	mucopolysaccharidose type III	205:233	It has beneficial effects for patients with mucopolysaccharidose type III through inhibiting glycosaminoglycan biosynthesis.					
29949794	6	47	theme	Genistein	812:820	arg1	treatment					822:830	RESULTS Genistein treatment	804:830	RESULTS Genistein treatment	804:830	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	0	48	theme	Type-Specific	67:79	arg1	Manner					81:86	a Cell Type-Specific Manner	60:86	a Cell Type-Specific Manner	60:86	Genistein Enhances or Reduces Glycosaminoglycan Quantity in a Cell Type-Specific Manner.					
29949794	4	49	theme	HCT116	475:480	arg1	cells					482:486	CHOK1, CHO3.1, CHO3.3, and HCT116 cells	448:486	cells	482:486	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
29949794	6	50	theme	HCT116	912:917	arg1	cells					919:923	HCT116 cells	912:923	HCT116 cells	912:923	RESULTS Genistein treatment reduced the amount of glycans but increased the amount of glycosaminoglycans in HCT116 cells.					
29949794	7	51	theme	glycosaminoglycan	983:999	arg1	quantities					1001:1010	both glycan and glycosaminoglycan quantities	967:1010	quantities	1001:1010	In contrast, genistein treatment reduced both glycan and glycosaminoglycan quantities in CHOK1, CHO3.1, and CHO3.3 cells in addition to differential changes in glycosaminoglycan disaccharide compositions.					
29949794	4	52	dep	CHO3.3	463:468	arg1	CHO3.1					455:460	CHOK1, CHO3.1, CHO3.3, and HCT116 cells	448:486	CHO3.1	455:460	METHODS To understand the underlying molecular mechanisms, CHOK1, CHO3.1, CHO3.3, and HCT116 cells were treated with genistein and the monosaccharide compositions and quantity of all glycans from the cell lysate were measured after thorough acid hydrolysis followed by HPLC analysis.					
30646817	3	0	from	state	576:580	arg1	matter					601:606	this matter	596:606	this matter	596:606	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	6	1	theme	derived	804:810	arg1	PrPSc					812:816	brain derived PrPSc	798:816	brain derived PrPSc	798:816	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	8	2	theme	infectious	1000:1009	arg1	amyloid					1021:1027	the infectious PrP23-144 amyloid	996:1027	the infectious PrP23-144 amyloid	996:1027	On the other hand, the infectious PrP23-144 amyloid exhibits a PIRIBS architecture.					
30646817	2	3	theme	PrPSc	293:297	arg1	structure					299:307	the PrPSc structure	289:307	the PrPSc structure	289:307	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	1	4	theme	sine	113:116	arg1	qua					118:120	a sine qua	111:120	a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers	111:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	3	5	theme	art	589:591	arg1	state					576:580	the state	572:580	the state of the art in this matter	572:606	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	0	6	theme	PrPSc	50:54	arg1	structure					37:45	the structure	33:45	The prion 2018 round tables (I): the structure of PrPSc.	0:55	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	1	7	theme	process	203:209	arg1	barriers					258:265	the strain phenomenon and transmission barriers	219:265	barriers	258:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	1	7	theme	process	203:209	arg1	phenomenon					230:239	the strain phenomenon and transmission barriers	219:265	phenomenon	230:239	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	1	7	theme	process	203:209	arg1	features					186:193	critical features	177:193	critical features of that process such as the strain phenomenon and transmission barriers	177:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	1	7	theme	process	203:209	arg1	propagation					155:165	PrPSc propagation	149:165	PrPSc propagation	149:165	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	6	8	theme	experimental	770:781	arg1	constraints					783:793	experimental constraints	770:793	experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies	770:874	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	9	9	theme	structure	1084:1092	arg1	types					1075:1079	both types	1070:1079	both types of structure	1070:1092	Perhaps, both types of structure co-exist.					
30646817	3	10	theme	commentary	440:449	arg1	article					451:457	This commentary article	435:457	This commentary article	435:457	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	2	11	theme	difficulties	326:337	arg1	full					318:321	full	318:321	full	318:321	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	6	12	dep	derived	804:810	arg1	brain					798:802	brain	798:802	brain	798:802	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	1	13	theme	non	122:124	arg1	qua					118:120	a sine qua	111:120	a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers	111:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	0	14	theme	prion	4:8	arg1	I					29:29	I	29:29	I	29:29	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	0	14	theme	prion	4:8	arg1	tables					21:26	The prion 2018 round tables	0:26	The prion 2018 round tables (I): the structure of PrPSc.	0:55	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	6	15	theme	X-ray	844:848	arg1	diffraction					856:866	X-ray fiber diffraction	844:866	X-ray fiber diffraction	844:866	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	0	16	theme	round	15:19	arg1	I					29:29	I	29:29	I	29:29	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	0	16	theme	round	15:19	arg1	tables					21:26	The prion 2018 round tables	0:26	The prion 2018 round tables (I): the structure of PrPSc.	0:55	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	8	17	theme	PIRIBS	1040:1045	arg1	architecture					1047:1058	a PIRIBS architecture	1038:1058	a PIRIBS architecture	1038:1058	On the other hand, the infectious PrP23-144 amyloid exhibits a PIRIBS architecture.					
30646817	6	18	link	derived	804:810	arg1	PrPSc					812:816	brain derived PrPSc	798:816	brain derived PrPSc	798:816	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	2	19	theme	large	354:358	arg1	amount					360:365	a large amount	352:365	a large amount of structural information that allows us to begin to understand it	352:432	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	2	19	theme	large	354:358	arg1	information					381:391	structural information	370:391	structural information	370:391	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	5	20	contain	have	706:709	arg1	Both					693:696	Both	693:696	Both	693:696	Both of them have relevant features.					
30646817	5	20	contain	have	706:709	arg1	them					701:704	them	701:704	them	701:704	Both of them have relevant features.					
30646817	5	20	contain	have	706:709	arg2	features					720:727	relevant features	711:727	relevant features	711:727	Both of them have relevant features.					
30646817	3	21	theme	round	472:476	arg1	table					478:482	a round table	470:482	a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter	470:606	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	0	22	theme	2018	10:13	arg1	I					29:29	I	29:29	I	29:29	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	0	22	theme	2018	10:13	arg1	tables					21:26	The prion 2018 round tables	0:26	The prion 2018 round tables (I): the structure of PrPSc.	0:55	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	6	23	theme	fiber	850:854	arg1	diffraction					856:866	X-ray fiber diffraction	844:866	X-ray fiber diffraction	844:866	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	4	24	theme	PrPSc	635:639	arg1	models					625:630	Two alternative models	609:630	Two alternative models of PrPSc	609:639	Two alternative models of PrPSc exist: the PIRIBS and the 4-rung β-solenoid models.					
30646817	5	25	theme	relevant	711:718	arg1	features					720:727	relevant features	711:727	relevant features	711:727	Both of them have relevant features.					
30646817	1	26	theme	strain	223:228	arg1	phenomenon					230:239	the strain phenomenon and transmission barriers	219:265	phenomenon	230:239	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	6	27	theme	β-solenoid	741:750	arg1	model					752:756	The 4-rung β-solenoid model	730:756	The 4-rung β-solenoid model	730:756	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	1	28	theme	PrPSc	149:153	arg1	propagation					155:165	PrPSc propagation	149:165	PrPSc propagation	149:165	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	7	29	theme	brain-derived	956:968	arg1	PrPSc					970:974	brain-derived PrPSc	956:974	brain-derived PrPSc	956:974	Furthermore, it allows facile accommodation of the bulky glycans that decorate brain-derived PrPSc.					
30646817	6	30	theme	4-rung	734:739	arg1	model					752:756	The 4-rung β-solenoid model	730:756	The 4-rung β-solenoid model	730:756	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	3	31	theme	Prion	511:515	arg1	2018					517:520	the Prion 2018 meeting	507:528	the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter	507:606	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	2	32	theme	information	381:391	arg1	amount					360:365	a large amount	352:365	a large amount of structural information that allows us to begin to understand it	352:432	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	2	32	theme	information	381:391	arg1	information					381:391	structural information	370:391	structural information	370:391	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	7	33	theme	glycans	934:940	arg1	accommodation					907:919	facile accommodation	900:919	facile accommodation of the bulky glycans that decorate brain-derived PrPSc	900:974	Furthermore, it allows facile accommodation of the bulky glycans that decorate brain-derived PrPSc.					
30646817	6	34	dep	cryo-EM	832:838	arg1	studies					868:874	studies	868:874	studies	868:874	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	2	35	theme	structural	370:379	arg1	information					381:391	structural information	370:391	structural information	370:391	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	1	36	theme	transmission	245:256	arg1	barriers					258:265	the strain phenomenon and transmission barriers	219:265	barriers	258:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	0	37	dep	tables	21:26	arg1	structure					37:45	the structure	33:45	The prion 2018 round tables (I): the structure of PrPSc.	0:55	The prion 2018 round tables (I): the structure of PrPSc.					
30646817	4	38	theme	β-solenoid	674:683	arg1	models					685:690	the 4-rung β-solenoid models	663:690	the 4-rung β-solenoid models	663:690	Two alternative models of PrPSc exist: the PIRIBS and the 4-rung β-solenoid models.					
30646817	8	39	theme	PrP23-144	1011:1019	arg1	amyloid					1021:1027	the infectious PrP23-144 amyloid	996:1027	the infectious PrP23-144 amyloid	996:1027	On the other hand, the infectious PrP23-144 amyloid exhibits a PIRIBS architecture.					
30646817	4	40	theme	4-rung	667:672	arg1	models					685:690	the 4-rung β-solenoid models	663:690	the 4-rung β-solenoid models	663:690	Two alternative models of PrPSc exist: the PIRIBS and the 4-rung β-solenoid models.					
30646817	7	41	theme	facile	900:905	arg1	accommodation					907:919	facile accommodation	900:919	facile accommodation of the bulky glycans that decorate brain-derived PrPSc	900:974	Furthermore, it allows facile accommodation of the bulky glycans that decorate brain-derived PrPSc.					
30646817	1	42	theme	PrPSc	88:92	arg1	structure					75:83	the structure	71:83	the structure of PrPSc	71:92	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	3	43	dep	Santiago	538:545	arg1	Compostela					550:559	Santiago de Compostela	538:559	Santiago de Compostela	538:559	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	7	44	theme	bulky	928:932	arg1	glycans					934:940	the bulky glycans	924:940	the bulky glycans	924:940	Furthermore, it allows facile accommodation of the bulky glycans that decorate brain-derived PrPSc.					
30646817	7	45	link	brain-derived	956:968	arg1	PrPSc					970:974	brain-derived PrPSc	956:974	brain-derived PrPSc	956:974	Furthermore, it allows facile accommodation of the bulky glycans that decorate brain-derived PrPSc.					
30646817	4	46	theme	alternative	613:623	arg1	models					625:630	Two alternative models	609:630	Two alternative models of PrPSc	609:639	Two alternative models of PrPSc exist: the PIRIBS and the 4-rung β-solenoid models.					
30646817	2	47	contain	have	347:350	arg1	we					340:341	we	340:341	we	340:341	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	2	47	contain	have	347:350	arg2	information					381:391	structural information	370:391	structural information	370:391	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	2	47	contain	have	347:350	arg2	amount					360:365	a large amount	352:365	a large amount of structural information that allows us to begin to understand it	352:432	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	6	48	theme	PrPSc	812:816	arg1	constraints					783:793	experimental constraints	770:793	experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies	770:874	The 4-rung β-solenoid model agrees with experimental constraints of brain derived PrPSc obtained from cryo-EM and X-ray fiber diffraction studies.					
30646817	3	49	theme	meeting	522:528	arg1	2018					517:520	the Prion 2018 meeting	507:528	the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter	507:606	This commentary article summarizes a round table that took place within the Prion 2018 meeting held in Santiago de Compostela to discuss the state of the art in this matter.					
30646817	2	50	theme	structure	299:307	arg1	elucidation					274:284	elucidation	274:284	elucidation of the PrPSc structure	274:307	While elucidation of the PrPSc structure has been full of difficulties, we now have a large amount of structural information that allows us to begin to understand it.					
30646817	8	51	theme	other	984:988	arg1	hand					990:993	the other hand	980:993	the other hand	980:993	On the other hand, the infectious PrP23-144 amyloid exhibits a PIRIBS architecture.					
30646817	1	52	theme	critical	177:184	arg1	phenomenon					230:239	the strain phenomenon and transmission barriers	219:265	phenomenon	230:239	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	1	52	theme	critical	177:184	arg1	features					186:193	critical features	177:193	critical features of that process such as the strain phenomenon and transmission barriers	177:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
30646817	1	52	theme	critical	177:184	arg1	barriers					258:265	the strain phenomenon and transmission barriers	219:265	barriers	258:265	Understanding the structure of PrPSc is without doubt a sine qua non to understand not only PrPSc propagation, but also critical features of that process such as the strain phenomenon and transmission barriers.					
31050875	9	0	theme	sensitive	1284:1292	arg1	platform					1343:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	7	1	theme	highest	994:1000	arg1	potential					1002:1010	the highest potential	990:1010	the highest potential to differentiate between population groups	990:1053	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	4	2	theme	assay	812:816	arg1	sensitivity					793:803	high sensitivity	788:803	high sensitivity of the assay	788:816	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	6	3	theme	mL-L	927:930	arg1	concentration					904:916	concentration	904:916	concentration of 50 µg mL-L	904:930	hTf is spotted at concentration of 50 µg mL-L .					
31050875	7	4	theme	population	1037:1046	arg1	groups					1048:1053	population groups	1037:1053	population groups	1037:1053	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	3	5	theme	colorectal	499:508	arg1	CRC					521:523	CRC	521:523	CRC	521:523	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	5	theme	colorectal	499:508	arg1	carcinoma					510:518	colorectal carcinoma	499:518	colorectal carcinoma (CRC) patients	499:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	6	6	theme	50 µg	921:925	arg1	mL-L					927:930	50 µg mL-L	921:930	50 µg mL-L	921:930	hTf is spotted at concentration of 50 µg mL-L .					
31050875	7	7	theme	Phaseolus	1117:1125	arg1	PHA-E					1137:1141	PHA-E	1137:1141	PHA-E	1137:1141	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	7	7	theme	Phaseolus	1117:1125	arg1	vulgaris					1127:1134	Phaseolus vulgaris	1117:1134	Phaseolus vulgaris (PHA-E)	1117:1142	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	9	8	theme	glycan	1395:1400	arg1	biomarkers					1440:1449	biomarkers	1440:1449	biomarkers of ageing or a disease	1440:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	8	theme	glycan	1395:1400	arg1	structures					1402:1411	hTf glycan structures	1391:1411	hTf glycan structures which can be considered as biomarkers of ageing or a disease	1391:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	7	9	dep	aurantia	1073:1080	arg1	lectins					1144:1150	lectins	1144:1150	lectins	1144:1150	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	3	10	theme	mellitus	473:480	arg1	type					482:485	mellitus type 2	473:487	diabetes mellitus type 2 (T2DM)	464:494	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	1	11	theme	PURPOSE	91:97	arg1	Disease					99:105	PURPOSE Disease	91:105	PURPOSE Disease	91:105	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	3	12	theme	healthy	430:436	arg1	persons					438:444	healthy persons	430:444	healthy persons of different age	430:461	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	9	13	theme	protein	1324:1330	arg1	platform					1343:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	14	theme	lectin-based	1311:1322	arg1	platform					1343:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	2	15	attach	attached	331:338	arg3	form					376:379	their native form	363:379	their native form	363:379	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	15	attach	attached	331:338	arg2	glycans					322:328	hTf glycans	318:328	hTf glycans	318:328	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	15	attach	attached	331:338	arg1	core					355:358	the protein core	343:358	the protein core	343:358	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	4	16	theme	high	788:791	arg1	sensitivity					793:803	high sensitivity	788:803	high sensitivity of the assay	788:816	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	3	17	theme	DESIGN	395:400	arg1	molecules					406:414	EXPERIMENTAL DESIGN hTf molecules	382:414	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients	382:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	1	18	theme	glycosylation	179:191	arg1	pattern					193:199	human transferrin (hTf) glycosylation pattern	155:199	human transferrin (hTf) glycosylation pattern	155:199	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	3	19	theme	EXPERIMENTAL	382:393	arg1	molecules					406:414	EXPERIMENTAL DESIGN hTf molecules	382:414	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients	382:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	8	20	theme	initial	1157:1163	arg1	low					1209:1211	low	1209:1211	low	1209:1211	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	20	theme	initial	1157:1163	arg1	pg					1217:1218	75 pg	1214:1218	75 pg	1214:1218	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	20	theme	initial	1157:1163	arg1	hTf					1175:1177	hTf	1175:1177	hTf	1175:1177	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	20	theme	initial	1157:1163	arg1	amount					1165:1170	The initial amount	1153:1170	The initial amount of hTf which can be analyzed	1153:1199	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	9	21	theme	ageing	1454:1459	arg1	biomarkers					1440:1449	biomarkers	1440:1449	biomarkers of ageing or a disease	1440:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	21	theme	ageing	1454:1459	arg1	structures					1402:1411	hTf glycan structures	1391:1411	hTf glycan structures which can be considered as biomarkers of ageing or a disease	1391:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	1	22	theme	pattern	193:199	arg1	alteration					141:150	alteration	141:150	alteration of human transferrin (hTf) glycosylation pattern	141:199	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	0	23	theme	Diagnostic	0:9	arg1	Potential					11:19	Diagnostic Potential	0:19	Diagnostic Potential of Transferrin	0:34	Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.					
31050875	9	24	from	changes	1380:1386	arg1	biomarkers					1440:1449	biomarkers	1440:1449	biomarkers of ageing or a disease	1440:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	24	from	changes	1380:1386	arg1	structures					1402:1411	hTf glycan structures	1391:1411	hTf glycan structures which can be considered as biomarkers of ageing or a disease	1391:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	2	25	theme	glycans	322:328	arg1	investigation					301:313	the investigation	297:313	the investigation of hTf glycans, attached to the protein core in their native form	297:379	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	4	26	dep	criteria	614:621	arg1	ratio					689:693	i) signal-to-noise ratio	670:693	three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay	608:816	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	4	27	dep	units	745:749	arg1	ensuring					779:786	ensuring	779:786	ensuring high sensitivity of the assay	779:816	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	0	28	theme	Transferrin	24:34	arg1	Potential					11:19	Diagnostic Potential	0:19	Diagnostic Potential of Transferrin	0:34	Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.					
31050875	8	29	dep	low	1209:1211	arg1	low					1209:1211	low	1209:1211	low	1209:1211	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	29	dep	low	1209:1211	arg1	pg					1217:1218	75 pg	1214:1218	75 pg	1214:1218	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	29	dep	low	1209:1211	arg1	hTf					1175:1177	hTf	1175:1177	hTf	1175:1177	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	29	dep	low	1209:1211	arg1	amount					1165:1170	The initial amount	1153:1170	The initial amount of hTf which can be analyzed	1153:1199	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	7	30	dep	potential	1002:1010	arg1	differentiate					1015:1027	differentiate	1015:1027	to differentiate between population groups	1012:1053	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	2	31	theme	hTf	318:320	arg1	glycans					322:328	hTf glycans	318:328	hTf glycans	318:328	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	3	32	used	used	539:542	arg2	molecules					406:414	EXPERIMENTAL DESIGN hTf molecules	382:414	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients	382:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	33	theme	method	548:553	arg1	validation					555:564	method validation	548:564	method validation	548:564	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	4	34	theme	arbitrary	735:743	arg1	units					745:749	250 arbitrary units	731:749	250 arbitrary units	731:749	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	9	35	theme	disease	1466:1472	arg1	biomarkers					1440:1449	biomarkers	1440:1449	biomarkers of ageing or a disease	1440:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	35	theme	disease	1466:1472	arg1	structures					1402:1411	hTf glycan structures	1391:1411	hTf glycan structures which can be considered as biomarkers of ageing or a disease	1391:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	1	36	theme	human	155:159	arg1	transferrin					161:171	human transferrin	155:171	human transferrin (hTf) glycosylation pattern	155:199	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	1	36	theme	human	155:159	arg1	hTf					174:176	hTf	174:176	hTf	174:176	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	0	37	theme	Lectin-Based	49:60	arg1	Approach					81:88	Lectin-Based Protein Microarray Approach	49:88	Lectin-Based Protein Microarray Approach	49:88	Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.					
31050875	4	38	theme	hTf-lectin	645:654	arg1	interactions					656:667	hTf-lectin interactions	645:667	hTf-lectin interactions	645:667	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	3	39	attach	isolated	416:423	arg2	molecules					406:414	EXPERIMENTAL DESIGN hTf molecules	382:414	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients	382:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	39	attach	isolated	416:423	arg1	patients					526:533	colorectal carcinoma (CRC) patients	499:533	colorectal carcinoma (CRC) patients	499:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	39	attach	isolated	416:423	arg1	diabetes					464:471	diabetes mellitus type 2	464:487	diabetes mellitus type 2 (T2DM)	464:494	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	39	attach	isolated	416:423	arg1	persons					438:444	healthy persons	430:444	healthy persons of different age	430:461	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	39	attach	isolated	416:423	arg1	T2DM					490:493	T2DM	490:493	T2DM	490:493	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	4	40	theme	hTf	761:763	arg1	concentration					765:777	hTf concentration	761:777	hTf concentration	761:777	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	3	41	theme	age	459:461	arg1	patients					526:533	colorectal carcinoma (CRC) patients	499:533	colorectal carcinoma (CRC) patients	499:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	41	theme	age	459:461	arg1	diabetes					464:471	diabetes mellitus type 2	464:487	diabetes mellitus type 2 (T2DM)	464:494	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	41	theme	age	459:461	arg1	persons					438:444	healthy persons	430:444	healthy persons of different age	430:461	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	41	theme	age	459:461	arg1	T2DM					490:493	T2DM	490:493	T2DM	490:493	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	5	42	theme	RESULTS	819:825	arg1	lectins					831:837	RESULTS Six lectins	819:837	RESULTS Six lectins	819:837	RESULTS Six lectins, out of fourteen tested, satisfy the criteria.					
31050875	2	43	theme	protein	247:253	arg1	microarray					255:264	lectin-based protein microarray	234:264	lectin-based protein microarray	234:264	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	43	theme	protein	247:253	arg1	platform					224:231	A specific analytical platform	202:231	A specific analytical platform	202:231	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	4	44	theme	signal	708:713	arg1	intensity					715:723	3, ii) signal intensity	701:723	intensity	715:723	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	0	45	theme	Microarray	70:79	arg1	Approach					81:88	Lectin-Based Protein Microarray Approach	49:88	Lectin-Based Protein Microarray Approach	49:88	Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.					
31050875	9	46	theme	microarray	1332:1341	arg1	platform					1343:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	a very sensitive, high-throughput lectin-based protein microarray platform	1277:1350	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	2	47	theme	lectin-based	234:245	arg1	microarray					255:264	lectin-based protein microarray	234:264	lectin-based protein microarray	234:264	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	47	theme	lectin-based	234:245	arg1	platform					224:231	A specific analytical platform	202:231	A specific analytical platform	202:231	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	48	theme	protein	347:353	arg1	core					355:358	the protein core	343:358	the protein core	343:358	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	0	49	theme	Protein	62:68	arg1	Approach					81:88	Lectin-Based Protein Microarray Approach	49:88	Lectin-Based Protein Microarray Approach	49:88	Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.					
31050875	9	50	theme	RELEVANCE	1246:1254	arg1	Results					1256:1262	CONCLUSION AND CLINICAL RELEVANCE Results	1222:1262	CONCLUSION AND CLINICAL RELEVANCE Results	1222:1262	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	51	dep	sensitive	1284:1292	arg1	high-throughput					1295:1309	high-throughput	1295:1309	high-throughput	1295:1309	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	4	52	theme	signal-to-noise	673:687	arg1	ratio					689:693	i) signal-to-noise ratio	670:693	three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay	608:816	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	7	53	theme	isolated	962:969	arg1	samples					953:959	physiological samples	939:959	physiological samples (isolated hTf)	939:974	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	7	53	theme	isolated	962:969	arg1	hTf					971:973	isolated hTf	962:973	isolated hTf	962:973	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	2	54	theme	analytical	213:222	arg1	microarray					255:264	lectin-based protein microarray	234:264	lectin-based protein microarray	234:264	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	54	theme	analytical	213:222	arg1	platform					224:231	A specific analytical platform	202:231	A specific analytical platform	202:231	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	9	55	theme	CONCLUSION	1222:1231	arg1	Results					1256:1262	CONCLUSION AND CLINICAL RELEVANCE Results	1222:1262	CONCLUSION AND CLINICAL RELEVANCE Results	1222:1262	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	4	56	theme	interactions	656:667	arg1	evaluation					631:640	the evaluation	627:640	the evaluation of hTf-lectin interactions	627:667	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	9	57	theme	hTf	1391:1393	arg1	biomarkers					1440:1449	biomarkers	1440:1449	biomarkers of ageing or a disease	1440:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	9	57	theme	hTf	1391:1393	arg1	structures					1402:1411	hTf glycan structures	1391:1411	hTf glycan structures which can be considered as biomarkers of ageing or a disease	1391:1472	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31050875	1	58	theme	specific	112:119	arg1	condition					121:129	a specific condition	110:129	a specific condition	110:129	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	4	59	theme	results	586:592	arg1	Reliability					567:577	Reliability	567:577	Reliability of the results	567:592	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	7	60	theme	physiological	939:951	arg1	samples					953:959	physiological samples	939:959	physiological samples (isolated hTf)	939:974	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	7	60	theme	physiological	939:951	arg1	hTf					971:973	isolated hTf	962:973	isolated hTf	962:973	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	2	61	theme	specific	204:211	arg1	microarray					255:264	lectin-based protein microarray	234:264	lectin-based protein microarray	234:264	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	2	61	theme	specific	204:211	arg1	platform					224:231	A specific analytical platform	202:231	A specific analytical platform	202:231	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	3	62	theme	carcinoma	510:518	arg1	patients					526:533	colorectal carcinoma (CRC) patients	499:533	colorectal carcinoma (CRC) patients	499:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	7	63	dep	Triticum	1089:1096	arg1	vulgaris					1098:1105	Triticum vulgaris (WGA)	1089:1111	Triticum vulgaris (WGA)	1089:1111	When physiological samples (isolated hTf) are analyzed, the highest potential to differentiate between population groups expresses Aleuria aurantia (AAL), Triticum vulgaris (WGA) and Phaseolus vulgaris (PHA-E) lectins.					
31050875	8	64	theme	hTf	1175:1177	arg1	low					1209:1211	low	1209:1211	low	1209:1211	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	64	theme	hTf	1175:1177	arg1	pg					1217:1218	75 pg	1214:1218	75 pg	1214:1218	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	64	theme	hTf	1175:1177	arg1	hTf					1175:1177	hTf	1175:1177	hTf	1175:1177	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	8	64	theme	hTf	1175:1177	arg1	amount					1165:1170	The initial amount	1153:1170	The initial amount of hTf which can be analyzed	1153:1199	The initial amount of hTf which can be analyzed is very low (75 pg).					
31050875	3	65	dep	diabetes	464:471	arg1	type					482:485	mellitus type 2	473:487	diabetes mellitus type 2 (T2DM)	464:494	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	4	66	dep	ensuring	779:786	arg1	iii					756:758	iii	756:758	iii	756:758	Reliability of the results is ensured by three criteria for the evaluation of hTf-lectin interactions: i) signal-to-noise ratio above 3, ii) signal intensity above 250 arbitrary units, and iii) hTf concentration ensuring high sensitivity of the assay.					
31050875	2	67	theme	native	369:374	arg1	form					376:379	their native form	363:379	their native form	363:379	A specific analytical platform, lectin-based protein microarray, is designed and optimized for the investigation of hTf glycans, attached to the protein core in their native form.					
31050875	3	68	theme	different	449:457	arg1	age					459:461	different age	449:461	different age	449:461	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	3	69	theme	hTf	402:404	arg1	molecules					406:414	EXPERIMENTAL DESIGN hTf molecules	382:414	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients	382:533	EXPERIMENTAL DESIGN hTf molecules isolated from healthy persons of different age, diabetes mellitus type 2 (T2DM) or colorectal carcinoma (CRC) patients are used for method validation.					
31050875	1	70	theme	transferrin	161:171	arg1	pattern					193:199	human transferrin (hTf) glycosylation pattern	155:199	human transferrin (hTf) glycosylation pattern	155:199	PURPOSE Disease or a specific condition may cause alteration of human transferrin (hTf) glycosylation pattern.					
31050875	9	71	theme	CLINICAL	1237:1244	arg1	RELEVANCE					1246:1254	CLINICAL RELEVANCE	1237:1254	CLINICAL RELEVANCE	1237:1254	CONCLUSION AND CLINICAL RELEVANCE Results confirm that a very sensitive, high-throughput lectin-based protein microarray platform can be formulated to detect changes in hTf glycan structures which can be considered as biomarkers of ageing or a disease.					
31084922	9	0	theme	healthy	1583:1589	arg1	donors					1591:1596	healthy donors	1583:1596	healthy donors	1583:1596	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	2	1	gly	O-glycosylated	296:309	arg1	IgA1					277:280	Serum IgA1	271:280	Serum IgA1	271:280	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	2	1	gly	O-glycosylated	296:309	arg1	nephropathy					318:328	IgA nephropathy	314:328	IgA nephropathy	314:328	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	2	1	gly	O-glycosylated	296:309	arg2	IgA1					277:280	Serum IgA1	271:280	Serum IgA1	271:280	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	3	2	link	enzyme-linked	461:473	arg1	assay					489:493	lectin enzyme-linked immunosorbent assay	454:493	lectin enzyme-linked immunosorbent assay	454:493	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	2	3	from	nephropathy	318:328	arg1	O-glycosylated					296:309	O-glycosylated	296:309	O-glycosylated	296:309	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	4	4	theme	effective	603:611	arg1	spectrometry					584:595	Mass spectrometry	579:595	Mass spectrometry	579:595	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	4	4	theme	effective	603:611	arg1	means					613:617	an effective means	600:617	an effective means of quantification of O-glycans	600:648	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	10	5	attach	attachment	1714:1723	arg2	fell					1697:1700	GalNAc fell	1690:1700	GalNAc fell	1690:1700	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	10	5	attach	attachment	1714:1723	arg1	GalNAc					1728:1733	GalNAc	1728:1733	GalNAc	1728:1733	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	1	6	theme	BACKGROUND	98:107	arg1	deposition					120:129	BACKGROUND Glomerular deposition	98:129	BACKGROUND Glomerular deposition of IgA1	98:137	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	6	theme	BACKGROUND	98:107	arg1	feature					151:157	a common feature	142:157	a common feature of Henoch-Schönlein purpura nephritis (HSPN)	142:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	10	7	theme	Gal	1710:1712	arg1	attachment					1714:1723	the Gal attachment	1706:1723	the Gal attachment to GalNAc	1706:1733	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	3	8	theme	exact	536:540	arg1	information					553:563	the exact structural information	532:563	the exact structural information of O-glycans	532:576	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	5	9	with	recipients	822:831	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	9	with	recipients	822:831	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	10	theme	other	873:877	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	10	theme	other	873:877	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	11	theme	healthy	917:923	arg1	donors					925:930	26 normal healthy donors	907:930	26 normal healthy donors	907:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	3	12	theme	O-glycans	568:576	arg1	information					553:563	the exact structural information	532:563	the exact structural information of O-glycans	532:576	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	5	13	theme	IgAN	837:840	arg1	recipients					842:851	26 IgAN recipients	834:851	26 IgAN recipients	834:851	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	9	14	theme	Gal/GalNAc	1497:1506	arg1	ratio					1508:1512	the Gal/GalNAc ratio	1493:1512	the Gal/GalNAc ratio of HSPN recipients	1493:1531	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	9	14	theme	Gal/GalNAc	1497:1506	arg1	lower					1552:1556	lower	1552:1556	lower	1552:1556	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	7	15	theme	GalNAc	1154:1159	arg1	percentage					1140:1149	The percentage	1136:1149	The percentage of GalNAc 3 in HSPN recipients	1136:1180	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	15	theme	GalNAc	1154:1159	arg1	higher					1200:1205	higher	1200:1205	higher	1200:1205	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	5	16	from	recipients	842:851	arg1	IgA1					805:808	serum IgA1	799:808	serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	799:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	10	17	theme	healthy	1785:1791	arg1	donors					1793:1798	healthy donors	1785:1798	healthy donors	1785:1798	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	1	18	theme	common	144:149	arg1	deposition					120:129	BACKGROUND Glomerular deposition	98:129	BACKGROUND Glomerular deposition of IgA1	98:137	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	18	theme	common	144:149	arg1	feature					151:157	a common feature	142:157	a common feature of Henoch-Schönlein purpura nephritis (HSPN)	142:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	10	19	theme	purpura	1643:1649	arg1	recipients					1651:1660	Henoch-Schönlein purpura recipients	1626:1660	Henoch-Schönlein purpura recipients	1626:1660	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	4	20	theme	IgA1	686:689	arg1	O-glycans					691:699	IgA1 O-glycans	686:699	IgA1 O-glycans	686:699	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	7	21	theme	OKDs	1220:1223	arg1	recipients					1225:1234	OKDs recipients	1220:1234	OKDs recipients	1220:1234	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	2	22	from	O-glycosylated	296:309	arg1	nephropathy					318:328	IgA nephropathy	314:328	IgA nephropathy	314:328	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	6	23	theme	combinations	986:997	arg1	RESULTS					957:963	RESULTS	957:963	RESULTS Of the 14 GalNac-Gal combinations	957:997	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	1	24	theme	purpura	179:185	arg1	HSPN					198:201	HSPN	198:201	HSPN	198:201	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	24	theme	purpura	179:185	arg1	nephritis					187:195	Henoch-Schönlein purpura nephritis	162:195	Henoch-Schönlein purpura nephritis (HSPN)	162:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	0	25	from	Evaluation	0:9	arg1	Nephritis					63:71	Henoch-Schönlein Purpura Nephritis	38:71	Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry	38:95	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	9	26	theme	HSPN	1517:1520	arg1	recipients					1522:1531	HSPN recipients	1517:1531	HSPN recipients	1517:1531	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	5	27	from	recipients	822:831	arg1	IgA1					805:808	serum IgA1	799:808	serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	799:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	3	28	theme	immunosorbent	475:487	arg1	assay					489:493	lectin enzyme-linked immunosorbent assay	454:493	lectin enzyme-linked immunosorbent assay	454:493	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	8	29	theme	GalNAc	1325:1330	arg1	lower					1371:1375	lower	1371:1375	lower	1371:1375	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	8	29	theme	GalNAc	1325:1330	arg1	percentage					1311:1320	the percentage	1307:1320	the percentage of GalNAc 5 in HSPN recipients	1307:1351	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	10	30	theme	CONCLUSIONS	1599:1609	arg1	Examination					1611:1621	CONCLUSIONS Examination	1599:1621	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients	1599:1660	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	2	31	theme	glomerular	374:383	arg1	injury					385:390	glomerular injury	374:390	glomerular injury	374:390	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	5	32	theme	HSPN	817:820	arg1	recipients					822:831	7 HSPN recipients	815:831	7 HSPN recipients	815:831	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	3	33	located	detected	431:438	arg1	HSPN					443:446	HSPN	443:446	HSPN	443:446	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	3	33	located	detected	431:438	arg2	IgA1					417:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	1	34	theme	Glomerular	109:118	arg1	deposition					120:129	BACKGROUND Glomerular deposition	98:129	BACKGROUND Glomerular deposition of IgA1	98:137	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	34	theme	Glomerular	109:118	arg1	feature					151:157	a common feature	142:157	a common feature of Henoch-Schönlein purpura nephritis (HSPN)	142:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	5	35	with	recipients	842:851	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	35	with	recipients	842:851	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	3	36	theme	enzyme-linked	461:473	arg1	assay					489:493	lectin enzyme-linked immunosorbent assay	454:493	lectin enzyme-linked immunosorbent assay	454:493	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	0	37	theme	IgA1	14:17	arg1	O-glycosylation					19:33	IgA1 O-glycosylation	14:33	IgA1 O-glycosylation	14:33	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	7	38	dep	donors	1248:1253	arg1	P =					1256:1258	P =	1256:1258	P =	1256:1258	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	38	dep	donors	1248:1253	arg1	P <					1270:1272	P <	1270:1272	P <	1270:1272	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	38	dep	donors	1248:1253	arg1	donors					1248:1253	healthy donors	1240:1253	healthy donors (P = .0027 and P < .0001, respectively)	1240:1293	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	39	theme	HSPN	1166:1169	arg1	recipients					1171:1180	HSPN recipients	1166:1180	HSPN recipients	1166:1180	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	5	40	theme	serum	799:803	arg1	IgA1					805:808	serum IgA1	799:808	serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	799:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	3	41	theme	Abnormal	393:400	arg1	IgA1					417:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	11	42	theme	IgA1	1805:1808	arg1	similar					1851:1857	similar	1851:1857	similar	1851:1857	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	11	42	theme	IgA1	1805:1808	arg1	profile					1826:1832	The IgA1 O-glycosylation profile	1801:1832	The IgA1 O-glycosylation profile of HSPN	1801:1840	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	0	43	theme	Purpura	55:61	arg1	Nephritis					63:71	Henoch-Schönlein Purpura Nephritis	38:71	Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry	38:95	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	9	44	theme	OKDs	1563:1566	arg1	recipients					1568:1577	OKDs recipients	1563:1577	OKDs recipients	1563:1577	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	10	45	theme	fell	1697:1700	arg1	attachment					1714:1723	the Gal attachment	1706:1723	the Gal attachment to GalNAc	1706:1733	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	10	45	theme	fell	1697:1700	arg1	number					1680:1685	the number	1676:1685	the number of GalNAc fell	1676:1700	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	4	46	theme	quantification	622:635	arg1	spectrometry					584:595	Mass spectrometry	579:595	Mass spectrometry	579:595	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	4	46	theme	quantification	622:635	arg1	means					613:617	an effective means	600:617	an effective means of quantification of O-glycans	600:648	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	6	47	theme	combination	1073:1083	arg1	percentage					1037:1046	the percentage	1033:1046	the percentage of the only 6GalNAc-2Gal combination	1033:1083	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	6	47	theme	combination	1073:1083	arg1	different					1103:1111	different	1103:1111	different	1103:1111	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	3	48	theme	structural	542:551	arg1	information					553:563	the exact structural information	532:563	the exact structural information of O-glycans	532:576	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	2	49	theme	Serum	271:275	arg1	IgA1					277:280	Serum IgA1	271:280	Serum IgA1	271:280	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	1	50	theme	nephritis	187:195	arg1	deposition					120:129	BACKGROUND Glomerular deposition	98:129	BACKGROUND Glomerular deposition of IgA1	98:137	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	50	theme	nephritis	187:195	arg1	feature					151:157	a common feature	142:157	a common feature of Henoch-Schönlein purpura nephritis (HSPN)	142:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	5	51	with	donors	925:930	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	51	with	donors	925:930	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	2	52	theme	IgA	314:316	arg1	nephropathy					318:328	IgA nephropathy	314:328	IgA nephropathy	314:328	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	8	53	theme	healthy	1410:1416	arg1	donors					1418:1423	healthy donors	1410:1423	healthy donors (P = .0008, P < .0001, respectively)	1410:1460	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	4	54	theme	O-glycans	640:648	arg1	quantification					622:635	quantification	622:635	quantification of O-glycans	622:648	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	10	55	theme	kidney	1765:1770	arg1	diseases					1772:1779	other kidney diseases	1759:1779	other kidney diseases	1759:1779	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	1	56	theme	IgA1	134:137	arg1	deposition					120:129	BACKGROUND Glomerular deposition	98:129	BACKGROUND Glomerular deposition of IgA1	98:137	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	56	theme	IgA1	134:137	arg1	feature					151:157	a common feature	142:157	a common feature of Henoch-Schönlein purpura nephritis (HSPN)	142:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	11	57	gly	O-glycosylation	1810:1824	arg1	HSPN					1837:1840	HSPN	1837:1840	HSPN	1837:1840	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	1	58	from	that	234:237	arg1	indistinguishable					211:227	indistinguishable	211:227	indistinguishable	211:227	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	6	59	theme	mass	1014:1017	arg1	spectrometry					1019:1030	mass spectrometry	1014:1030	mass spectrometry	1014:1030	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	5	60	dep	MATERIALS	734:742	arg1	investigated					759:770	investigated	759:770	investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	759:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	61	theme	mass	938:941	arg1	spectrometry					943:954	mass spectrometry	938:954	mass spectrometry	938:954	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	4	62	theme	mass	715:718	arg1	spectrometry					720:731	mass spectrometry	715:731	mass spectrometry	715:731	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	0	63	theme	Mass	79:82	arg1	Spectrometry					84:95	Mass Spectrometry	79:95	Mass Spectrometry	79:95	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	5	64	theme	kidney	879:884	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	64	theme	kidney	879:884	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	65	theme	O-glycosylation	772:786	arg1	profile					788:794	O-glycosylation profile	772:794	O-glycosylation profile	772:794	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	66	from	recipients	857:866	arg1	IgA1					805:808	serum IgA1	799:808	serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	799:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	1	67	theme	Henoch-Schönlein	162:177	arg1	HSPN					198:201	HSPN	198:201	HSPN	198:201	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	67	theme	Henoch-Schönlein	162:177	arg1	nephritis					187:195	Henoch-Schönlein purpura nephritis	162:195	Henoch-Schönlein purpura nephritis (HSPN)	162:202	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	6	68	theme	GalNac-Gal	975:984	arg1	combinations					986:997	the 14 GalNac-Gal combinations	968:997	the 14 GalNac-Gal combinations	968:997	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	7	69	from	percentage	1140:1149	arg1	recipients					1171:1180	HSPN recipients	1166:1180	HSPN recipients	1166:1180	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	3	70	gly	O-glycosylated	402:415	arg1	IgA1					417:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	9	71	theme	Gal	1477:1479	arg1	lower					1552:1556	lower	1552:1556	lower	1552:1556	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	9	71	theme	Gal	1477:1479	arg1	content					1481:1487	the Gal content	1473:1487	the Gal content	1473:1487	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	6	72	theme	6GalNAc-2Gal	1060:1071	arg1	combination					1073:1083	the only 6GalNAc-2Gal combination	1051:1083	the only 6GalNAc-2Gal combination	1051:1083	RESULTS Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN.					
31084922	2	73	theme	injury	385:390	arg1	development					359:369	the development	355:369	the development of glomerular injury	355:390	Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury.					
31084922	5	74	theme	normal	910:915	arg1	donors					925:930	26 normal healthy donors	907:930	26 normal healthy donors	907:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	11	75	theme	HSPN	1837:1840	arg1	similar					1851:1857	similar	1851:1857	similar	1851:1857	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	11	75	theme	HSPN	1837:1840	arg1	profile					1826:1832	The IgA1 O-glycosylation profile	1801:1832	The IgA1 O-glycosylation profile of HSPN	1801:1840	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	0	76	theme	O-glycosylation	19:33	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.	0:96	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	4	77	theme	Mass	579:582	arg1	spectrometry					584:595	Mass spectrometry	579:595	Mass spectrometry	579:595	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	4	77	theme	Mass	579:582	arg1	means					613:617	an effective means	600:617	an effective means of quantification of O-glycans	600:648	Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry.					
31084922	8	78	theme	HSPN	1337:1340	arg1	recipients					1342:1351	HSPN recipients	1337:1351	HSPN recipients	1337:1351	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	10	79	theme	Henoch-Schönlein	1626:1641	arg1	recipients					1651:1660	Henoch-Schönlein purpura recipients	1626:1660	Henoch-Schönlein purpura recipients	1626:1660	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	0	80	theme	Henoch-Schönlein	38:53	arg1	Nephritis					63:71	Henoch-Schönlein Purpura Nephritis	38:71	Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry	38:95	Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.					
31084922	9	81	theme	recipients	1522:1531	arg1	ratio					1508:1512	the Gal/GalNAc ratio	1493:1512	the Gal/GalNAc ratio of HSPN recipients	1493:1531	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	9	81	theme	recipients	1522:1531	arg1	lower					1552:1556	lower	1552:1556	lower	1552:1556	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	9	81	theme	recipients	1522:1531	arg1	content					1481:1487	the Gal content	1473:1487	the Gal content	1473:1487	Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors.					
31084922	3	82	theme	lectin	454:459	arg1	assay					489:493	lectin enzyme-linked immunosorbent assay	454:493	lectin enzyme-linked immunosorbent assay	454:493	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	8	83	dep	donors	1418:1423	arg1	P =					1426:1428	P =	1426:1428	P =	1426:1428	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	8	83	dep	donors	1418:1423	arg1	P <					1437:1439	P <	1437:1439	P <	1437:1439	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	3	84	theme	O-glycosylated	402:415	arg1	IgA1					417:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1	393:420	Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans.					
31084922	11	85	theme	O-glycosylation	1810:1824	arg1	similar					1851:1857	similar	1851:1857	similar	1851:1857	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	11	85	theme	O-glycosylation	1810:1824	arg1	profile					1826:1832	The IgA1 O-glycosylation profile	1801:1832	The IgA1 O-glycosylation profile of HSPN	1801:1840	The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.					
31084922	5	86	from	donors	925:930	arg1	IgA1					805:808	serum IgA1	799:808	serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors	799:930	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	10	87	theme	other	1759:1763	arg1	diseases					1772:1779	other kidney diseases	1759:1779	other kidney diseases	1759:1779	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	5	88	with	recipients	857:866	arg1	OKDs					896:899	OKDs	896:899	OKDs	896:899	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	5	88	with	recipients	857:866	arg1	diseases					886:893	other kidney diseases	873:893	other kidney diseases (OKDs)	873:900	MATERIALS AND METHODS We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry.					
31084922	10	89	theme	recipients	1651:1660	arg1	Examination					1611:1621	CONCLUSIONS Examination	1599:1621	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients	1599:1660	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	8	90	from	percentage	1311:1320	arg1	recipients					1342:1351	HSPN recipients	1337:1351	HSPN recipients	1337:1351	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	7	91	theme	healthy	1240:1246	arg1	P =					1256:1258	P =	1256:1258	P =	1256:1258	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	91	theme	healthy	1240:1246	arg1	P <					1270:1272	P <	1270:1272	P <	1270:1272	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	7	91	theme	healthy	1240:1246	arg1	donors					1248:1253	healthy donors	1240:1253	healthy donors (P = .0027 and P < .0001, respectively)	1240:1293	The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively).					
31084922	8	92	theme	OKDs	1390:1393	arg1	recipients					1395:1404	OKDs recipients	1390:1404	OKDs recipients	1390:1404	Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively).					
31084922	10	93	theme	GalNAc	1690:1695	arg1	fell					1697:1700	GalNAc fell	1690:1700	GalNAc fell	1690:1700	CONCLUSIONS Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors.					
31084922	1	94	theme	IgA	247:249	arg1	IgAN					264:267	IgAN	264:267	IgAN	264:267	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
31084922	1	94	theme	IgA	247:249	arg1	nephropathy					251:261	IgA nephropathy	247:261	IgA nephropathy (IgAN)	247:268	BACKGROUND Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN).					
29325159	6	0	theme	triple	877:882	arg1	mutant					884:889	the Arabidopsis gux1/2/3 triple mutant	852:889	the Arabidopsis gux1/2/3 triple mutant	852:889	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	9	1	theme	xylosyl	1621:1627	arg1	chains					1634:1639	xylosyl side chains	1621:1639	xylosyl side chains	1621:1639	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	2	from	present	1306:1312	arg1	xylan					1339:1343	wild-type Arabidopsis xylan	1317:1343	wild-type Arabidopsis xylan	1317:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	3	theme	xylosyl	1218:1224	arg1	residues					1226:1233	the side chain xylosyl residues	1203:1233	the side chain xylosyl residues	1203:1233	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	8	4	dep	Oryza	1466:1470	arg1	arabinosyltransferase2					1485:1506	xylan arabinosyltransferase2	1479:1506	Oryza sativa xylan arabinosyltransferase2	1466:1506	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	8	4	dep	Oryza	1466:1470	arg1	sativa					1472:1477	Oryza sativa xylan arabinosyltransferase2	1466:1506	Oryza sativa xylan arabinosyltransferase2	1466:1506	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	10	5	from	assay	1877:1881	arg1	findings					1779:1786	Our findings	1775:1786	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay	1775:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	6	6	theme	Arabidopsis	856:866	arg1	mutant					884:889	the Arabidopsis gux1/2/3 triple mutant	852:889	the Arabidopsis gux1/2/3 triple mutant	852:889	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	4	7	theme	sativa	574:579	arg1	XYXT1					608:612	XYXT1	608:612	XYXT1 (xylan xylosyltransferase1)	608:640	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	4	7	theme	sativa	574:579	arg1	glycosyltransferase					587:605	a rice (Oryza sativa) GT61 glycosyltransferase	560:605	a rice (Oryza sativa) GT61 glycosyltransferase	560:605	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	9	8	theme	xylooligomer	1646:1657	arg1	acceptors					1659:1667	xylooligomer acceptors	1646:1667	xylooligomer acceptors	1646:1667	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	9	theme	structural	1051:1060	arg1	characterization					1062:1077	comprehensive structural characterization	1037:1077	comprehensive structural characterization	1037:1077	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	2	10	theme	residues	249:256	arg1	backbone					216:223	a linear backbone	207:223	a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides	207:322	It consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides.					
29325159	1	11	theme	secondary	158:166	arg1	walls					168:172	both primary and secondary walls	141:172	both primary and secondary walls of grass species	141:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	0	12	theme	Side	70:73	arg1	Chains					75:80	2-O-Xylosyl Side Chains	58:80	2-O-Xylosyl Side Chains	58:80	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	10	13	theme	chains	1981:1986	arg1	addition					1956:1963	the addition	1952:1963	the addition of xylosyl side chains onto xylan	1952:1997	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	9	14	theme	recombinant	1678:1688	arg1	OsXAT2					1690:1695	recombinant OsXAT2	1678:1695	recombinant OsXAT2	1678:1695	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	10	15	theme	xylosyl	1968:1974	arg1	chains					1981:1986	xylosyl side chains	1968:1986	xylosyl side chains	1968:1986	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	7	16	theme	linkage	1016:1022	arg1	analysis					1024:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	1	17	theme	species	183:189	arg1	walls					168:172	both primary and secondary walls	141:172	both primary and secondary walls of grass species	141:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	10	18	dep	in	1839:1840	arg1	vitro					1842:1846	vitro	1842:1846	vitro	1842:1846	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	10	19	theme	in	1801:1802	arg1	study					1826:1830	an in vivo gain-of-function study	1798:1830	an in vivo gain-of-function study	1798:1830	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	8	20	theme	xylan	1422:1426	arg1	chains					1412:1417	the arabinosyl side chains	1392:1417	the arabinosyl side chains of xylan	1392:1426	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	9	21	contain	possessed	1586:1594	arg2	activity					1599:1606	an activity	1596:1606	an activity transferring xylosyl side chains onto xylooligomer acceptors	1596:1667	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	9	21	contain	possessed	1586:1594	arg1	XYXT1					1580:1584	recombinant XYXT1	1568:1584	recombinant XYXT1	1568:1584	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	9	22	theme	arabinosyl	1723:1732	arg1	chains					1739:1744	arabinosyl side chains	1723:1744	arabinosyl side chains	1723:1744	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	23	theme	Arabidopsis	1327:1337	arg1	xylan					1339:1343	wild-type Arabidopsis xylan	1317:1343	wild-type Arabidopsis xylan	1317:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	2	24	theme	β-1,4-linked	228:239	arg1	residues					249:256	β-1,4-linked xylosyl residues	228:256	β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides	228:322	It consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides.					
29325159	1	25	theme	major	118:122	arg1	Xylan					107:111	Xylan	107:111	Xylan	107:111	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	1	25	theme	major	118:122	arg1	hemicellulose					124:136	a major hemicellulose	116:136	a major hemicellulose in both primary and secondary walls of grass species	116:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	9	26	theme	chains	1739:1744	arg1	addition					1711:1718	the addition	1707:1718	the addition of arabinosyl side chains onto xylooligomer acceptors	1707:1772	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	27	theme	chain	1212:1216	arg1	residues					1226:1233	the side chain xylosyl residues	1203:1233	the side chain xylosyl residues	1203:1233	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	10	28	theme	gain-of-function	1809:1824	arg1	study					1826:1830	an in vivo gain-of-function study	1798:1830	an in vivo gain-of-function study	1798:1830	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	9	29	theme	xylooligomer	1751:1762	arg1	acceptors					1764:1772	xylooligomer acceptors	1751:1772	xylooligomer acceptors	1751:1772	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	0	30	theme	Chains	75:80	arg1	Addition					46:53	the Addition	42:53	the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone	42:104	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	1	31	theme	primary	146:152	arg1	walls					168:172	both primary and secondary walls	141:172	both primary and secondary walls of grass species	141:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	1	32	from	hemicellulose	124:136	arg1	walls					168:172	both primary and secondary walls	141:172	both primary and secondary walls of grass species	141:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	6	33	theme	sugar	932:936	arg1	substitutions					938:950	sugar substitutions	932:950	sugar substitutions	932:950	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	8	34	theme	xylan	1479:1483	arg1	arabinosyltransferase2					1485:1506	xylan arabinosyltransferase2	1479:1506	Oryza sativa xylan arabinosyltransferase2	1466:1506	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	7	35	theme	present	1306:1312	arg1	O-2					1283:1285	O-2	1283:1285	O-2	1283:1285	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	35	theme	present	1306:1312	arg1	substituent					1290:1300	a substituent	1288:1300	a substituent not present in wild-type Arabidopsis xylan	1288:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	3	36	theme	Xylosyl	325:331	arg1	substitutions					333:345	Xylosyl substitutions	325:345	Xylosyl substitutions directly on the xylan backbone	325:376	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	7	37	theme	Arabidopsis	1153:1163	arg1	plants					1165:1170	transgenic Arabidopsis plants	1142:1170	transgenic Arabidopsis plants expressing XYXT1	1142:1187	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	10	38	from	study	1826:1830	arg1	findings					1779:1786	Our findings	1775:1786	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay	1775:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	4	39	theme	functional	529:538	arg1	characterization					540:555	functional characterization	529:555	functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions	529:677	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	0	40	theme	Rice	8:11	arg1	Xylosyltransferase					13:30	A Novel Rice Xylosyltransferase	0:30	A Novel Rice Xylosyltransferase	0:30	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	3	41	theme	grass	404:408	arg1	species					410:416	grass species	404:416	grass species	404:416	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	8	42	theme	triple	1525:1530	arg1	mutant					1532:1537	the gux1/2/3 triple mutant	1512:1537	the gux1/2/3 triple mutant	1512:1537	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	10	43	theme	recombinant	1848:1858	arg1	assay					1877:1881	an in vitro recombinant protein activity assay	1836:1881	an in vitro recombinant protein activity assay	1836:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	6	44	theme	xylosyl	975:981	arg1	chains					988:993	xylosyl side chains	975:993	xylosyl side chains	975:993	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	5	45	theme	different	728:736	arg1	organs					743:748	different rice organs	728:748	different rice organs	728:748	XYXT1 was found to be ubiquitously expressed in different rice organs and its encoded protein was targeted to the Golgi, the site for xylan biosynthesis.					
29325159	7	46	theme	xylanase	1109:1116	arg1	digestion					1118:1126	xylanase digestion	1109:1126	xylanase digestion of xylan	1109:1135	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	47	from	O-2	1283:1285	arg1	backbone					1271:1278	the xylan backbone	1261:1278	the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan	1261:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	48	theme	xylan	1265:1269	arg1	backbone					1271:1278	the xylan backbone	1261:1278	the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan	1261:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	3	49	theme	grass	468:472	arg1	species					474:480	grass species	468:480	grass species	468:480	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	0	50	theme	2-O-Xylosyl	58:68	arg1	Chains					75:80	2-O-Xylosyl Side Chains	58:80	2-O-Xylosyl Side Chains	58:80	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	10	51	theme	activity	1868:1875	arg1	assay					1877:1881	an in vitro recombinant protein activity assay	1836:1881	an in vitro recombinant protein activity assay	1836:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	8	52	theme	side	1407:1410	arg1	chains					1412:1417	the arabinosyl side chains	1392:1417	the arabinosyl side chains of xylan	1392:1426	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	5	53	theme	xylan	814:818	arg1	biosynthesis					820:831	xylan biosynthesis	814:831	xylan biosynthesis	814:831	XYXT1 was found to be ubiquitously expressed in different rice organs and its encoded protein was targeted to the Golgi, the site for xylan biosynthesis.					
29325159	4	54	theme	glycosyltransferase	587:605	arg1	characterization					540:555	functional characterization	529:555	functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions	529:677	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	6	55	from	mutant	884:889	arg1	devoid					922:927	devoid	922:927	devoid	922:927	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	7	56	theme	xylooligomers	1082:1094	arg1	analysis					1024:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	56	theme	xylooligomers	1082:1094	arg1	characterization					1062:1077	comprehensive structural characterization	1037:1077	comprehensive structural characterization	1037:1077	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	6	57	theme	gux1/2/3	868:875	arg1	mutant					884:889	the Arabidopsis gux1/2/3 triple mutant	852:889	the Arabidopsis gux1/2/3 triple mutant	852:889	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	9	58	theme	side	1629:1632	arg1	chains					1634:1639	xylosyl side chains	1621:1639	xylosyl side chains	1621:1639	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	10	59	theme	in	1839:1840	arg1	assay					1877:1881	an in vitro recombinant protein activity assay	1836:1881	an in vitro recombinant protein activity assay	1836:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	4	60	theme	rice	562:565	arg1	XYXT1					608:612	XYXT1	608:612	XYXT1 (xylan xylosyltransferase1)	608:640	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	4	60	theme	rice	562:565	arg1	glycosyltransferase					587:605	a rice (Oryza sativa) GT61 glycosyltransferase	560:605	a rice (Oryza sativa) GT61 glycosyltransferase	560:605	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	4	61	from	role	651:654	arg1	substitutions					665:677	xylan substitutions	659:677	xylan substitutions	659:677	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	4	62	theme	Oryza	568:572	arg1	sativa					574:579	Oryza sativa	568:579	a rice (Oryza sativa) GT61 glycosyltransferase	560:605	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	7	63	theme	comprehensive	1037:1049	arg1	characterization					1062:1077	comprehensive structural characterization	1037:1077	comprehensive structural characterization	1037:1077	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	64	attach	attached	1249:1256	arg1	backbone					1271:1278	the xylan backbone	1261:1278	the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan	1261:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	64	attach	attached	1249:1256	arg2	residues					1226:1233	the side chain xylosyl residues	1203:1233	the side chain xylosyl residues	1203:1233	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	10	65	theme	side	1976:1979	arg1	chains					1981:1986	xylosyl side chains	1968:1986	xylosyl side chains	1968:1986	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	7	66	theme	Glycosyl	1007:1014	arg1	analysis					1024:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis	1007:1031	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	1	67	theme	grass	177:181	arg1	species					183:189	grass species	177:189	grass species	177:189	Xylan is a major hemicellulose in both primary and secondary walls of grass species.					
29325159	2	68	theme	linear	209:214	arg1	backbone					216:223	a linear backbone	207:223	a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides	207:322	It consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides.					
29325159	4	69	theme	xylan	615:619	arg1	XYXT1					608:612	XYXT1	608:612	XYXT1 (xylan xylosyltransferase1)	608:640	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	4	69	theme	xylan	615:619	arg1	xylosyltransferase1					621:639	xylan xylosyltransferase1	615:639	xylan xylosyltransferase1	615:639	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	2	70	theme	xylosyl	241:247	arg1	residues					249:256	β-1,4-linked xylosyl residues	228:256	β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides	228:322	It consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides.					
29325159	9	71	theme	side	1734:1737	arg1	chains					1739:1744	arabinosyl side chains	1723:1744	arabinosyl side chains	1723:1744	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	72	theme	side	1207:1210	arg1	residues					1226:1233	the side chain xylosyl residues	1203:1233	the side chain xylosyl residues	1203:1233	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	3	73	from	substitutions	333:345	arg1	backbone					369:376	the xylan backbone	359:376	the xylan backbone	359:376	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	7	74	theme	wild-type	1317:1325	arg1	xylan					1339:1343	wild-type Arabidopsis xylan	1317:1343	wild-type Arabidopsis xylan	1317:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	5	75	theme	rice	738:741	arg1	organs					743:748	different rice organs	728:748	different rice organs	728:748	XYXT1 was found to be ubiquitously expressed in different rice organs and its encoded protein was targeted to the Golgi, the site for xylan biosynthesis.					
29325159	4	76	theme	xylan	659:663	arg1	substitutions					665:677	xylan substitutions	659:677	xylan substitutions	659:677	Here, we report functional characterization of a rice (Oryza sativa) GT61 glycosyltransferase, XYXT1 (xylan xylosyltransferase1), for its role in xylan substitutions.					
29325159	0	77	theme	Xylan	91:95	arg1	Backbone					97:104	the Xylan Backbone	87:104	the Xylan Backbone	87:104	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	6	78	theme	substitutions	938:950	arg1	devoid					922:927	devoid	922:927	devoid	922:927	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	3	79	theme	xylan	363:367	arg1	backbone					369:376	the xylan backbone	359:376	the xylan backbone	359:376	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	2	80	link	β-1,4-linked	228:239	arg1	residues					249:256	β-1,4-linked xylosyl residues	228:256	β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides	228:322	It consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides.					
29325159	3	81	theme	responsible	429:439	arg1	genes					423:427	genes	423:427	genes responsible for xylan substitutions in grass species	423:480	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	0	82	theme	Novel	2:6	arg1	Xylosyltransferase					13:30	A Novel Rice Xylosyltransferase	0:30	A Novel Rice Xylosyltransferase	0:30	A Novel Rice Xylosyltransferase Catalyzes the Addition of 2-O-Xylosyl Side Chains onto the Xylan Backbone.					
29325159	7	83	from	xylan	1339:1343	arg1	present					1306:1312	present	1306:1312	present	1306:1312	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	8	84	theme	gux1/2/3	1516:1523	arg1	mutant					1532:1537	the gux1/2/3 triple mutant	1512:1537	the gux1/2/3 triple mutant	1512:1537	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	7	85	theme	xylan	1131:1135	arg1	digestion					1118:1126	xylanase digestion	1109:1126	xylanase digestion of xylan	1109:1135	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	10	86	theme	protein	1860:1866	arg1	assay					1877:1881	an in vitro recombinant protein activity assay	1836:1881	an in vitro recombinant protein activity assay	1836:1881	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	6	87	theme	side	983:986	arg1	chains					988:993	xylosyl side chains	975:993	xylosyl side chains	975:993	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	6	88	from	devoid	922:927	arg1	mutant					884:889	the Arabidopsis gux1/2/3 triple mutant	852:889	the Arabidopsis gux1/2/3 triple mutant	852:889	When expressed in the Arabidopsis gux1/2/3 triple mutant, in which xylan was completely devoid of sugar substitutions, XYXT1 was able to add xylosyl side chains onto xylan.					
29325159	3	89	theme	xylan	445:449	arg1	substitutions					451:463	xylan substitutions	445:463	xylan substitutions in grass species	445:480	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	5	90	theme	encoded	758:764	arg1	protein					766:772	its encoded protein	754:772	its encoded protein	754:772	XYXT1 was found to be ubiquitously expressed in different rice organs and its encoded protein was targeted to the Golgi, the site for xylan biosynthesis.					
29325159	9	91	theme	recombinant	1568:1578	arg1	XYXT1					1580:1584	recombinant XYXT1	1568:1584	recombinant XYXT1	1568:1584	Furthermore, we showed that recombinant XYXT1 possessed an activity transferring xylosyl side chains onto xylooligomer acceptors, whereas recombinant OsXAT2 catalyzed the addition of arabinosyl side chains onto xylooligomer acceptors.					
29325159	7	92	attach	present	1306:1312	arg2	substituent					1290:1300	a substituent	1288:1300	a substituent not present in wild-type Arabidopsis xylan	1288:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	92	attach	present	1306:1312	arg1	xylan					1339:1343	wild-type Arabidopsis xylan	1317:1343	wild-type Arabidopsis xylan	1317:1343	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	7	92	attach	present	1306:1312	arg2	O-2					1283:1285	O-2	1283:1285	O-2	1283:1285	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
29325159	8	93	theme	xylosyl	1370:1376	arg1	residues					1378:1385	xylosyl residues	1370:1385	xylosyl residues	1370:1385	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	3	94	from	substitutions	451:463	arg1	species					474:480	grass species	468:480	grass species	468:480	Xylosyl substitutions directly on the xylan backbone have not been reported in grass species, and genes responsible for xylan substitutions in grass species have not been well elucidated.					
29325159	10	95	dep	in	1801:1802	arg1	vivo					1804:1807	vivo	1804:1807	vivo	1804:1807	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	10	96	theme	novel	1911:1915	arg1	XYXT1					1900:1904	XYXT1	1900:1904	XYXT1	1900:1904	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	10	96	theme	novel	1911:1915	arg1	β-1,2-xylosyltransferase					1917:1940	a novel β-1,2-xylosyltransferase	1909:1940	a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan	1909:1997	Our findings from both an in vivo gain-of-function study and an in vitro recombinant protein activity assay demonstrate that XYXT1 is a novel β-1,2-xylosyltransferase mediating the addition of xylosyl side chains onto xylan.					
29325159	8	97	theme	arabinosyl	1396:1405	arg1	chains					1412:1417	the arabinosyl side chains	1392:1417	the arabinosyl side chains of xylan	1392:1426	XYXT1 was unable to add xylosyl residues onto the arabinosyl side chains of xylan when it was co-expressed with OsXAT2 (Oryza sativa xylan arabinosyltransferase2) in the gux1/2/3 triple mutant.					
29325159	7	98	theme	transgenic	1142:1151	arg1	plants					1165:1170	transgenic Arabidopsis plants	1142:1170	transgenic Arabidopsis plants expressing XYXT1	1142:1187	Glycosyl linkage analysis and comprehensive structural characterization of xylooligomers generated by xylanase digestion of xylan from transgenic Arabidopsis plants expressing XYXT1 revealed that the side chain xylosyl residues were directly attached to the xylan backbone at O-2, a substituent not present in wild-type Arabidopsis xylan.					
31826523	1	0	theme	acid	176:179	arg1	composites					155:164	Microencapsulated phase change material (MPCM) composites	108:164	Microencapsulated phase change material (MPCM) composites of capric acid	108:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	0	theme	acid	176:179	arg1	acid					176:179	capric acid	169:179	capric acid	169:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	5	1	theme	chemical	800:807	arg1	bonds					809:813	chemical bonds	800:813	chemical bonds	800:813	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	3	2	theme	scanning	545:552	arg1	DSC					567:569	DSC	567:569	DSC	567:569	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	2	theme	scanning	545:552	arg1	calorimeter					554:564	differential scanning calorimeter	532:564	differential scanning calorimeter (DSC)	532:570	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	2	3	theme	Mechanical	294:303	arg1	stabilities					317:327	Mechanical and thermal stabilities	294:327	Mechanical and thermal stabilities of these MPCM composites	294:352	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	5	4	theme	energy-dispersive	748:764	arg1	data					773:776	EDX (energy-dispersive X-ray) data	743:776	EDX (energy-dispersive X-ray) data	743:776	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	3	5	theme	differential	532:543	arg1	DSC					567:569	DSC	567:569	DSC	567:569	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	5	theme	differential	532:543	arg1	calorimeter					554:564	differential scanning calorimeter	532:564	differential scanning calorimeter (DSC)	532:570	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	5	6	theme	X-ray	766:770	arg1	data					773:776	EDX (energy-dispersive X-ray) data	743:776	EDX (energy-dispersive X-ray) data	743:776	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	6	7	theme	thermal	912:918	arg1	stability					920:928	thermal stability	912:928	thermal stability	912:928	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	8	theme	cross-link	1081:1090	arg1	reaction					1092:1099	cross-link reaction	1081:1099	cross-link reaction	1081:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	9	theme	process	1021:1027	arg1	parameters					1029:1038	process parameters	1021:1038	process parameters i.e. cross-linker amount and duration of cross-link reaction	1021:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	4	10	theme	electron	707:714	arg1	SEM					728:730	SEM	728:730	SEM	728:730	Morphology and particle size were analyzed using scanning electron microscope (SEM).					
31826523	4	10	theme	electron	707:714	arg1	microscope					716:725	scanning electron microscope	698:725	scanning electron microscope (SEM)	698:731	Morphology and particle size were analyzed using scanning electron microscope (SEM).					
31826523	5	11	theme	bonds	809:813	arg1	nature					790:795	nature	790:795	nature of chemical bonds	790:813	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	7	12	theme	heating/cooling	1202:1216	arg1	cycles					1218:1223	50 heating/cooling cycles	1199:1223	50 heating/cooling cycles	1199:1223	The composite tested and found stable for 50 heating/cooling cycles.					
31826523	3	13	theme	Thermal	435:441	arg1	enthalpy					455:462	enthalpy	455:462	enthalpy	455:462	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	13	theme	Thermal	435:441	arg1	melting/crystallization					469:491	melting/crystallization	469:491	melting/crystallization	469:491	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	13	theme	Thermal	435:441	arg1	properties					443:452	Thermal properties	435:452	Thermal properties (enthalpy and, melting/crystallization and cyclic tests)	435:509	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	6	14	theme	reaction	1092:1099	arg1	duration					1069:1076	duration	1069:1076	duration of cross-link reaction	1069:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	14	theme	reaction	1092:1099	arg1	reaction					1092:1099	cross-link reaction	1081:1099	cross-link reaction	1081:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	14	theme	reaction	1092:1099	arg1	amount					1058:1063	cross-linker amount	1045:1063	cross-linker amount	1045:1063	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	2	15	theme	silica	406:411	arg1	coating					413:419	silica coating	406:419	silica coating	406:419	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	6	16	theme	shell	965:969	arg1	ratio					971:975	the core: shell ratio	955:975	the core: shell ratio of 2:3	955:982	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	2	17	theme	thermal	309:315	arg1	stabilities					317:327	Mechanical and thermal stabilities	294:327	Mechanical and thermal stabilities of these MPCM composites	294:352	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	1	18	theme	Microencapsulated	108:124	arg1	MPCM					149:152	MPCM	149:152	MPCM	149:152	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	18	theme	Microencapsulated	108:124	arg1	material					139:146	Microencapsulated phase change material	108:146	Microencapsulated phase change material (MPCM) composites of capric acid	108:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	19	theme	protein	208:214	arg1	-polysaccharide					229:243	protein (Gelatin, GE)-polysaccharide	208:243	-polysaccharide	229:243	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	19	theme	protein	208:214	arg1	Arabic					250:255	Gum Arabic	246:255	Gum Arabic	246:255	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	6	20	theme	2:3	980:982	arg1	ratio					971:975	the core: shell ratio	955:975	the core: shell ratio of 2:3	955:982	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	3	21	dep	properties	443:452	arg1	properties					443:452	Thermal properties	435:452	Thermal properties (enthalpy and, melting/crystallization and cyclic tests)	435:509	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	21	dep	properties	443:452	arg1	enthalpy					455:462	enthalpy	455:462	enthalpy	455:462	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	21	dep	properties	443:452	arg1	melting/crystallization					469:491	melting/crystallization	469:491	melting/crystallization	469:491	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	21	dep	properties	443:452	arg1	tests					504:508	cyclic tests	497:508	cyclic tests	497:508	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	1	22	theme	phase	126:130	arg1	MPCM					149:152	MPCM	149:152	MPCM	149:152	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	22	theme	phase	126:130	arg1	material					139:146	Microencapsulated phase change material	108:146	Microencapsulated phase change material (MPCM) composites of capric acid	108:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	4	23	theme	scanning	698:705	arg1	SEM					728:730	SEM	728:730	SEM	728:730	Morphology and particle size were analyzed using scanning electron microscope (SEM).					
31826523	4	23	theme	scanning	698:705	arg1	microscope					716:725	scanning electron microscope	698:725	scanning electron microscope (SEM)	698:731	Morphology and particle size were analyzed using scanning electron microscope (SEM).					
31826523	0	24	theme	phase	47:51	arg1	composites					69:78	microencapsulated phase change material composites	29:78	microencapsulated phase change material composites for thermal energy storage	29:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	1	25	theme	shell	278:282	arg1	material					284:291	shell material	278:291	shell material	278:291	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	26	theme	change	132:137	arg1	MPCM					149:152	MPCM	149:152	MPCM	149:152	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	26	theme	change	132:137	arg1	material					139:146	Microencapsulated phase change material	108:146	Microencapsulated phase change material (MPCM) composites of capric acid	108:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	27	theme	Gelatin	217:223	arg1	-polysaccharide					229:243	protein (Gelatin, GE)-polysaccharide	208:243	-polysaccharide	229:243	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	27	theme	Gelatin	217:223	arg1	Arabic					250:255	Gum Arabic	246:255	Gum Arabic	246:255	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	0	28	theme	microencapsulated	29:45	arg1	composites					69:78	microencapsulated phase change material composites	29:78	microencapsulated phase change material composites for thermal energy storage	29:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	6	29	dep	amount	1058:1063	arg1	i.e.					1040:1043	i.e.	1040:1043	i.e.	1040:1043	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	1	30	theme	material	139:146	arg1	composites					155:164	Microencapsulated phase change material (MPCM) composites	108:164	Microencapsulated phase change material (MPCM) composites of capric acid	108:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	30	theme	material	139:146	arg1	acid					176:179	capric acid	169:179	capric acid	169:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	6	31	theme	cross-linker	1045:1056	arg1	reaction					1092:1099	cross-link reaction	1081:1099	cross-link reaction	1081:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	31	theme	cross-linker	1045:1056	arg1	amount					1058:1063	cross-linker amount	1045:1063	cross-linker amount	1045:1063	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	0	32	theme	material	60:67	arg1	composites					69:78	microencapsulated phase change material composites	29:78	microencapsulated phase change material composites for thermal energy storage	29:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	6	33	theme	encapsulation	1136:1148	arg1	ratio					1150:1154	encapsulation ratio	1136:1154	encapsulation ratio	1136:1154	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	1	34	dep	Arabic	250:255	arg1	GA					258:259	GA	258:259	GA	258:259	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	5	35	theme	X-Ray	868:872	arg1	XRD					863:865	XRD	863:865	XRD (X-Ray Diffraction)	863:885	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	5	35	theme	X-Ray	868:872	arg1	Diffraction					874:884	X-Ray Diffraction	868:884	X-Ray Diffraction	868:884	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	0	36	theme	change	53:58	arg1	composites					69:78	microencapsulated phase change material composites	29:78	microencapsulated phase change material composites for thermal energy storage	29:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	5	37	theme	EDX	743:745	arg1	data					773:776	EDX (energy-dispersive X-ray) data	743:776	EDX (energy-dispersive X-ray) data	743:776	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	1	38	dep	-polysaccharide	229:243	arg1	GE					226:227	protein (Gelatin, GE)-polysaccharide	208:243	GE	226:227	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	6	39	theme	parameters	1029:1038	arg1	role					1013:1016	the role	1009:1016	the role of process parameters i.e. cross-linker amount and duration of cross-link reaction	1009:1099	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	40	theme	core	959:962	arg1	ratio					971:975	the core: shell ratio	955:975	the core: shell ratio of 2:3	955:982	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	6	41	theme	surfactant	1106:1115	arg1	amount					1117:1122	surfactant amount	1106:1122	surfactant amount	1106:1122	Based on morphology and thermal stability, the composite made with the core: shell ratio of 2:3 was chosen for analyzing the role of process parameters i.e. cross-linker amount and duration of cross-link reaction, and surfactant amount influencing encapsulation ratio.					
31826523	2	42	theme	composites	343:352	arg1	stabilities					317:327	Mechanical and thermal stabilities	294:327	Mechanical and thermal stabilities of these MPCM composites	294:352	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	5	43	theme	crystalloid	821:831	arg1	structures					833:842	crystalloid structures	821:842	crystalloid structures	821:842	FTIR and EDX (energy-dispersive X-ray) data interpreted nature of chemical bonds while crystalloid structures were obtained from XRD (X-Ray Diffraction).					
31826523	3	44	theme	thermogravimetric	615:631	arg1	analyzer					633:640	thermogravimetric analyzer	615:640	thermogravimetric analyzer (TGA)	615:646	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	3	44	theme	thermogravimetric	615:631	arg1	TGA					643:645	TGA	643:645	TGA	643:645	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	2	45	theme	MPCM	338:341	arg1	composites					343:352	these MPCM composites	332:352	these MPCM composites	332:352	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	3	46	theme	thermal	579:585	arg1	stability					587:595	thermal stability	579:595	thermal stability	579:595	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	1	47	theme	-polysaccharide	229:243	arg1	interactions					262:273	protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions	208:273	protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions	208:273	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	0	48	theme	energy	92:97	arg1	storage					99:105	thermal energy storage	84:105	thermal energy storage	84:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	3	49	theme	cyclic	497:502	arg1	tests					504:508	cyclic tests	497:508	cyclic tests	497:508	Thermal properties (enthalpy and, melting/crystallization and cyclic tests) were estimated using differential scanning calorimeter (DSC) while, thermal stability was obtained from thermogravimetric analyzer (TGA).					
31826523	2	50	theme	glutaraldehyde	374:387	arg1	cross-linker					389:400	glutaraldehyde cross-linker	374:400	glutaraldehyde cross-linker	374:400	Mechanical and thermal stabilities of these MPCM composites were achieved using glutaraldehyde cross-linker and silica coating respectively.					
31826523	1	51	theme	Gum	246:248	arg1	-polysaccharide					229:243	protein (Gelatin, GE)-polysaccharide	208:243	-polysaccharide	229:243	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	1	51	theme	Gum	246:248	arg1	Arabic					250:255	Gum Arabic	246:255	Gum Arabic	246:255	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
31826523	0	52	theme	thermal	84:90	arg1	storage					99:105	thermal energy storage	84:105	thermal energy storage	84:105	Protein-polysaccharide based microencapsulated phase change material composites for thermal energy storage.					
31826523	4	53	theme	particle	664:671	arg1	size					673:676	particle size	664:676	particle size	664:676	Morphology and particle size were analyzed using scanning electron microscope (SEM).					
31826523	1	54	theme	capric	169:174	arg1	acid					176:179	capric acid	169:179	capric acid	169:179	Microencapsulated phase change material (MPCM) composites of capric acid were synthesized utilizing protein (Gelatin, GE)-polysaccharide (Gum Arabic, GA) interactions as shell material.					
30448401	7	0	theme	N-glycan	1341:1348	arg1	branching					1350:1358	decreased N-glycan branching	1331:1358	decreased N-glycan branching	1331:1358	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	2	1	from	inhibition	412:421	arg1	Golgi					476:480	the Golgi	472:480	the Golgi	472:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	2	2	theme	N-glycan-processing	347:365	arg1	deficiency					367:376	N-glycan-processing deficiency	347:376	N-glycan-processing deficiency	347:376	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	8	3	theme	ocular	1596:1601	arg1	diseases					1614:1621	ocular autoimmune diseases	1596:1621	ocular autoimmune diseases	1596:1621	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	1	4	theme	tissue	165:170	arg1	function					172:179	tissue function	165:179	tissue function	165:179	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	3	5	from	structures	584:593	arg1	cells					638:642	differentiated human corneal epithelial cells	598:642	differentiated human corneal epithelial cells	598:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	5	6	theme	biantennary	1051:1061	arg1	structures					1063:1072	biantennary structures	1051:1072	biantennary structures	1051:1072	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	7	7	with	patients	1205:1212	arg1	pemphigoid					1242:1251	ocular mucous membrane pemphigoid	1219:1251	ocular mucous membrane pemphigoid	1219:1251	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	2	8	theme	new	297:299	arg1	role					301:304	a new role	295:304	a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi	295:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	3	9	theme	corneal	619:625	arg1	cells					638:642	differentiated human corneal epithelial cells	598:642	differentiated human corneal epithelial cells	598:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	7	10	from	expression	1316:1325	arg1	conjunctiva					1367:1377	the conjunctiva	1363:1377	the conjunctiva	1363:1377	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	8	11	theme	enzymes	1504:1510	arg1	expression					1470:1479	the expression	1466:1479	the expression of N-glycan-processing enzymes in the Golgi	1466:1523	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	6	12	theme	N-glycan	1175:1182	arg1	branching					1184:1192	impaired N-glycan branching	1166:1192	impaired N-glycan branching	1166:1192	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	4	13	theme	glycogenes	763:772	arg1	expression					749:758	the expression	745:758	the expression of glycogenes involved in the biosynthesis of N-glycans	745:814	Remarkably, significant differences were detected between the efficacies of cytokines in regulating the expression of glycogenes involved in the biosynthesis of N-glycans.					
30448401	2	14	from	Golgi	476:480	arg1	inhibition					412:421	inhibition	412:421	inhibition of N-acetylglucosaminyltransferase expression in the Golgi	412:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	1	15	from	loss	157:160	arg1	diseases					202:209	ocular autoimmune diseases	184:209	ocular autoimmune diseases	184:209	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	3	16	theme	major	569:573	arg1	structures					584:593	major N-glycan structures	569:593	major N-glycan structures in differentiated human corneal epithelial cells	569:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	3	16	theme	major	569:573	arg1	oligosaccharides					533:548	complex-type biantennary oligosaccharides	508:548	complex-type biantennary oligosaccharides	508:548	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	5	17	theme	Golgi	934:938	arg1	pathway					950:956	the Golgi branching pathway	930:956	the Golgi branching pathway	930:956	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	1	18	theme	function	172:179	arg1	loss					157:160	a loss	155:160	a loss of tissue function in ocular autoimmune diseases	155:209	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	5	19	theme	necrosis	823:830	arg1	factor					832:837	Tumor necrosis factor α	817:839	Tumor necrosis factor α but not IL-1β	817:853	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	0	20	from	Deficiency	47:56	arg1	Disease					79:85	Ocular Autoimmune Disease	61:85	Ocular Autoimmune Disease	61:85	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	7	21	theme	ocular	1219:1224	arg1	pemphigoid					1242:1251	ocular mucous membrane pemphigoid	1219:1251	ocular mucous membrane pemphigoid	1219:1251	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	1	22	theme	High	88:91	arg1	levels					93:98	High levels	88:98	High levels of proinflammatory cytokines	88:127	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	6	23	theme	impaired	1166:1173	arg1	branching					1184:1192	impaired N-glycan branching	1166:1192	impaired N-glycan branching	1166:1192	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	4	24	theme	cytokines	721:729	arg1	efficacies					707:716	the efficacies	703:716	the efficacies of cytokines in regulating the expression of glycogenes involved in the biosynthesis of N-glycans	703:814	Remarkably, significant differences were detected between the efficacies of cytokines in regulating the expression of glycogenes involved in the biosynthesis of N-glycans.					
30448401	6	25	from	decrease	1080:1087	arg1	expression					1097:1106	gene expression	1092:1106	gene expression	1092:1106	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	2	26	theme	tumor	310:314	arg1	factor					325:330	tumor necrosis factor α	310:332	tumor necrosis factor α	310:332	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	6	27	theme	enzymatic	1143:1151	arg1	activity					1153:1160	enzymatic activity	1143:1160	enzymatic activity	1143:1160	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	1	28	theme	ocular	184:189	arg1	diseases					202:209	ocular autoimmune diseases	184:209	ocular autoimmune diseases	184:209	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	0	29	theme	Inflammatory	0:11	arg1	Stress					13:18	Inflammatory Stress	0:18	Inflammatory Stress	0:18	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	3	30	theme	human	613:617	arg1	cells					638:642	differentiated human corneal epithelial cells	598:642	differentiated human corneal epithelial cells	598:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	6	31	from	reduction	1130:1138	arg1	branching					1184:1192	impaired N-glycan branching	1166:1192	impaired N-glycan branching	1166:1192	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	6	31	from	reduction	1130:1138	arg1	activity					1153:1160	enzymatic activity	1143:1160	enzymatic activity	1143:1160	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	3	32	theme	epithelial	627:636	arg1	cells					638:642	differentiated human corneal epithelial cells	598:642	differentiated human corneal epithelial cells	598:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	1	33	theme	autoimmune	191:200	arg1	diseases					202:209	ocular autoimmune diseases	184:209	ocular autoimmune diseases	184:209	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	0	34	theme	N-Glycan	27:34	arg1	Processing					36:45	N-Glycan Processing	27:45	N-Glycan Processing Deficiency in Ocular Autoimmune Disease	27:85	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	4	35	theme	N-glycans	806:814	arg1	biosynthesis					790:801	the biosynthesis	786:801	the biosynthesis of N-glycans	786:814	Remarkably, significant differences were detected between the efficacies of cytokines in regulating the expression of glycogenes involved in the biosynthesis of N-glycans.					
30448401	1	36	theme	proinflammatory	103:117	arg1	cytokines					119:127	proinflammatory cytokines	103:127	proinflammatory cytokines	103:127	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	2	37	theme	N-acetylglucosaminyltransferase	426:456	arg1	expression					458:467	N-acetylglucosaminyltransferase expression	426:467	N-acetylglucosaminyltransferase expression in the Golgi	426:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	8	38	theme	autoimmune	1603:1612	arg1	diseases					1614:1621	ocular autoimmune diseases	1596:1621	ocular autoimmune diseases	1596:1621	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	1	39	theme	cytokines	119:127	arg1	levels					93:98	High levels	88:98	High levels of proinflammatory cytokines	88:127	High levels of proinflammatory cytokines have been associated with a loss of tissue function in ocular autoimmune diseases, but the basis for this relationship remains poorly understood.					
30448401	3	40	theme	mass	489:492	arg1	spectrometry					494:505	mass spectrometry	489:505	mass spectrometry	489:505	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	7	41	theme	membrane	1233:1240	arg1	pemphigoid					1242:1251	ocular mucous membrane pemphigoid	1219:1251	ocular mucous membrane pemphigoid	1219:1251	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	8	42	theme	proinflammatory	1415:1429	arg1	cytokines					1431:1439	proinflammatory cytokines	1415:1439	proinflammatory cytokines	1415:1439	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	7	43	theme	N-acetylglucosaminyltransferase	1284:1314	arg1	expression					1316:1325	marginal N-acetylglucosaminyltransferase expression	1275:1325	marginal N-acetylglucosaminyltransferase expression	1275:1325	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	7	44	theme	marginal	1275:1282	arg1	expression					1316:1325	marginal N-acetylglucosaminyltransferase expression	1275:1325	marginal N-acetylglucosaminyltransferase expression	1275:1325	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	8	45	theme	future	1547:1552	arg1	studies					1554:1560	future studies	1547:1560	future studies	1547:1560	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	5	46	theme	branching	940:948	arg1	pathway					950:956	the Golgi branching pathway	930:956	the Golgi branching pathway	930:956	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	5	47	theme	enzymes	910:916	arg1	expression					896:905	the expression	892:905	the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures	892:1072	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	0	48	theme	Processing	36:45	arg1	Deficiency					47:56	N-Glycan Processing Deficiency	27:56	N-Glycan Processing Deficiency in Ocular Autoimmune Disease	27:85	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	2	49	theme	expression	458:467	arg1	inhibition					412:421	inhibition	412:421	inhibition of N-acetylglucosaminyltransferase expression in the Golgi	412:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	2	50	theme	eye	400:402	arg1	surface					385:391	the surface	381:391	the surface of the eye	381:402	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	0	51	theme	Ocular	61:66	arg1	Disease					79:85	Ocular Autoimmune Disease	61:85	Ocular Autoimmune Disease	61:85	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	8	52	theme	diseases	1614:1621	arg1	pathophysiology					1577:1591	the pathophysiology	1573:1591	the pathophysiology of ocular autoimmune diseases	1573:1621	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	3	53	theme	complex-type	508:519	arg1	structures					584:593	major N-glycan structures	569:593	major N-glycan structures in differentiated human corneal epithelial cells	569:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	3	53	theme	complex-type	508:519	arg1	oligosaccharides					533:548	complex-type biantennary oligosaccharides	508:548	complex-type biantennary oligosaccharides	508:548	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	5	54	theme	profound	861:868	arg1	effect					870:875	a profound effect	859:875	a profound effect	859:875	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	8	55	from	expression	1470:1479	arg1	Golgi					1519:1523	the Golgi	1515:1523	the Golgi	1515:1523	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	3	56	theme	biantennary	521:531	arg1	structures					584:593	major N-glycan structures	569:593	major N-glycan structures in differentiated human corneal epithelial cells	569:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	3	56	theme	biantennary	521:531	arg1	oligosaccharides					533:548	complex-type biantennary oligosaccharides	508:548	complex-type biantennary oligosaccharides	508:548	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	7	57	theme	mucous	1226:1231	arg1	pemphigoid					1242:1251	ocular mucous membrane pemphigoid	1219:1251	ocular mucous membrane pemphigoid	1219:1251	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	7	58	from	branching	1350:1358	arg1	conjunctiva					1367:1377	the conjunctiva	1363:1377	the conjunctiva	1363:1377	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
30448401	5	59	theme	structures	1063:1072	arg1	formation					1038:1046	the formation	1034:1046	the formation of biantennary structures	1034:1072	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	3	60	theme	differentiated	598:611	arg1	cells					638:642	differentiated human corneal epithelial cells	598:642	differentiated human corneal epithelial cells	598:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	5	61	theme	Tumor	817:821	arg1	factor					832:837	Tumor necrosis factor α	817:839	Tumor necrosis factor α but not IL-1β	817:853	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	3	62	theme	N-glycan	575:582	arg1	structures					584:593	major N-glycan structures	569:593	major N-glycan structures in differentiated human corneal epithelial cells	569:642	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	3	62	theme	N-glycan	575:582	arg1	oligosaccharides					533:548	complex-type biantennary oligosaccharides	508:548	complex-type biantennary oligosaccharides	508:548	Using mass spectrometry, complex-type biantennary oligosaccharides were identified as major N-glycan structures in differentiated human corneal epithelial cells.					
30448401	5	63	contain	had	855:857	arg1	IL-1β					849:853	IL-1β	849:853	Tumor necrosis factor α but not IL-1β	817:853	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	5	63	contain	had	855:857	arg2	effect					870:875	a profound effect	859:875	a profound effect	859:875	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	5	63	contain	had	855:857	arg1	factor					832:837	Tumor necrosis factor α	817:839	Tumor necrosis factor α but not IL-1β	817:853	Tumor necrosis factor α but not IL-1β had a profound effect in suppressing the expression of enzymes involved in the Golgi branching pathway, including N-acetylglucosaminyltransferases 1 and 2, which are required for the formation of biantennary structures.					
30448401	2	64	theme	necrosis	316:323	arg1	factor					325:330	tumor necrosis factor α	310:332	tumor necrosis factor α	310:332	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	8	65	theme	N-glycan-processing	1484:1502	arg1	enzymes					1504:1510	N-glycan-processing enzymes	1484:1510	N-glycan-processing enzymes	1484:1510	Together, these data indicate that proinflammatory cytokines differentially influence the expression of N-glycan-processing enzymes in the Golgi and set the stage for future studies to explore the pathophysiology of ocular autoimmune diseases.					
30448401	4	66	theme	significant	657:667	arg1	differences					669:679	significant differences	657:679	significant differences	657:679	Remarkably, significant differences were detected between the efficacies of cytokines in regulating the expression of glycogenes involved in the biosynthesis of N-glycans.					
30448401	6	67	theme	gene	1092:1095	arg1	expression					1097:1106	gene expression	1092:1106	gene expression	1092:1106	This decrease in gene expression was correlated with a reduction in enzymatic activity and impaired N-glycan branching.					
30448401	0	68	theme	Autoimmune	68:77	arg1	Disease					79:85	Ocular Autoimmune Disease	61:85	Ocular Autoimmune Disease	61:85	Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.					
30448401	2	69	from	expression	458:467	arg1	Golgi					476:480	the Golgi	472:480	the Golgi	472:480	Here we investigate a new role for tumor necrosis factor α in promoting N-glycan-processing deficiency at the surface of the eye through inhibition of N-acetylglucosaminyltransferase expression in the Golgi.					
30448401	7	70	theme	decreased	1331:1339	arg1	branching					1350:1358	decreased N-glycan branching	1331:1358	decreased N-glycan branching	1331:1358	Moreover, patients with ocular mucous membrane pemphigoid were characterized by marginal N-acetylglucosaminyltransferase expression and decreased N-glycan branching in the conjunctiva.					
29729312	7	0	theme	interchain	1207:1216	arg1	bonds					1228:1232	the interchain disulfide bonds	1203:1232	the interchain disulfide bonds of IgG at pH 4.5	1203:1249	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	3	1	theme	acids	501:505	arg1	protein					480:486	a protein	478:486	a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites	478:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	4	2	from	similar	705:711	arg1	shape					716:720	shape	716:720	shape	716:720	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	2	from	similar	705:711	arg1	arrangement					737:747	positional arrangement	726:747	positional arrangement	726:747	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	6	3	theme	head	1019:1022	arg1	cells					1031:1035	spleen and head kidney cells	1008:1035	spleen and head kidney cells	1008:1035	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	0	4	theme	silver	101:106	arg1	Hypophthalmichthys					114:131	Hypophthalmichthys	114:131	Hypophthalmichthys	114:131	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	0	4	theme	silver	101:106	arg1	carp					108:111	silver carp	101:111	silver carp (Hypophthalmichthys molitrix)	101:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	8	5	theme	disulfide	1314:1322	arg1	bonds					1324:1328	disulfide bonds	1314:1328	disulfide bonds reduction	1314:1338	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	3	6	contain	possesses	512:520	arg1	protein					480:486	a protein	478:486	a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites	478:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	3	6	contain	possesses	512:520	arg2	motif					579:583	active-site CXXC motif	562:583	active-site CXXC motif	562:583	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	3	6	contain	possesses	512:520	arg2	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	3	6	contain	possesses	512:520	arg2	sequence					537:544	GILT signature sequence CQHGX2ECX2NX4C	522:559	GILT signature sequence CQHGX2ECX2NX4C	522:559	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	7	7	theme	Recombinant	1117:1127	arg1	ScGILT					1129:1134	Recombinant ScGILT	1117:1134	Recombinant ScGILT fused with a His6 tag	1117:1156	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	5	8	theme	head	895:898	arg1	kidney					900:905	head kidney	895:905	head kidney	895:905	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	8	theme	head	895:898	arg1	organs					876:881	fish immune organs	864:881	fish immune organs	864:881	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	3	9	theme	CXXC	574:577	arg1	motif					579:583	active-site CXXC motif	562:583	active-site CXXC motif	562:583	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	1	10	theme	class	288:292	arg1	antigens					308:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	7	11	from	pH	1244:1245	arg1	bonds					1228:1232	the interchain disulfide bonds	1203:1232	the interchain disulfide bonds of IgG at pH 4.5	1203:1249	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	6	12	theme	HeLa	1105:1108	arg1	cells					1110:1114	HeLa cells	1105:1114	HeLa cells	1105:1114	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	4	13	from	shape	716:720	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	13	from	shape	716:720	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	1	14	theme	II-restricted	294:306	arg1	antigens					308:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	6	15	with	stimulation	914:924	arg1	lipopolysaccharide					931:948	lipopolysaccharide	931:948	lipopolysaccharide	931:948	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	0	16	dep	Hypophthalmichthys	114:131	arg1	molitrix					133:140	Hypophthalmichthys molitrix	114:140	Hypophthalmichthys molitrix	114:140	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	4	17	theme	GILTs	688:692	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	17	theme	GILTs	688:692	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	5	18	theme	immune	869:874	arg1	kidney					900:905	head kidney	895:905	head kidney	895:905	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	18	theme	immune	869:874	arg1	organs					876:881	fish immune organs	864:881	fish immune organs	864:881	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	18	theme	immune	869:874	arg1	spleen					884:889	spleen	884:889	spleen	884:889	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	3	19	link	N-linked	604:611	arg1	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	3	20	theme	open	427:430	arg1	frame					440:444	Its open reading frame	423:444	Its open reading frame	423:444	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	2	21	theme	silver	410:415	arg1	carp					417:420	silver carp	410:420	silver carp	410:420	Herein, a GILT homolog (ScGILT) was identified from silver carp.					
29729312	5	22	theme	detected	816:823	arg1	tissues					825:831	all detected tissues	812:831	all detected tissues	812:831	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	6	23	theme	ScGILT	969:974	arg1	mRNA					976:979	ScGILT mRNA	969:979	ScGILT mRNA	969:979	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	7	24	theme	disulfide	1218:1226	arg1	bonds					1228:1232	the interchain disulfide bonds	1203:1232	the interchain disulfide bonds of IgG at pH 4.5	1203:1249	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	4	25	theme	ScGILT	671:676	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	25	theme	ScGILT	671:676	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	1	26	theme	important	213:221	arg1	role					223:226	an important role	210:226	an important role	210:226	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	0	27	theme	Molecular	0:8	arg1	characterization					25:40	Molecular and biological characterization	0:40	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).	0:142	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	1	28	theme	antigens	308:315	arg1	processing					235:244	the processing	231:244	the processing of major histocompatibility complex (MHC) class II-restricted antigens	231:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	4	29	theme	positional	726:735	arg1	arrangement					737:747	positional arrangement	726:747	positional arrangement	726:747	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	3	30	gly	glycosylation	613:625	arg2	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	3	30	gly	glycosylation	613:625	arg2	two					590:592	two	590:592	two	590:592	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	8	31	theme	II-restricted	1410:1422	arg1	antigens					1424:1431	MHC class II-restricted antigens	1400:1431	MHC class II-restricted antigens in silver carp	1400:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	8	32	theme	silver	1436:1441	arg1	carp					1443:1446	silver carp	1436:1446	silver carp	1436:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	3	33	theme	active-site	562:572	arg1	motif					579:583	active-site CXXC motif	562:583	active-site CXXC motif	562:583	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	4	34	theme	tertiary	648:655	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	34	theme	tertiary	648:655	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	0	35	theme	biological	14:23	arg1	characterization					25:40	Molecular and biological characterization	0:40	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).	0:142	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	6	36	theme	mRNA	976:979	arg1	expression					955:964	the expression	951:964	the expression of ScGILT mRNA	951:979	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	3	37	theme	signature	527:535	arg1	sequence					537:544	GILT signature sequence CQHGX2ECX2NX4C	522:559	GILT signature sequence CQHGX2ECX2NX4C	522:559	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	0	38	from	carp	108:111	arg1	characterization					25:40	Molecular and biological characterization	0:40	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).	0:142	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	3	39	theme	amino	495:499	arg1	acids					501:505	256 amino acids	491:505	256 amino acids	491:505	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	8	40	from	processing	1386:1395	arg1	carp					1443:1446	silver carp	1436:1446	silver carp	1436:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	4	41	theme	predicted	638:646	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	41	theme	predicted	638:646	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	0	42	theme	gamma-interferon-inducible	45:70	arg1	reductase					88:96	gamma-interferon-inducible lysosomal thiol reductase	45:96	gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix)	45:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	1	43	theme	disulfide	331:339	arg1	bonds					341:345	disulfide bonds	331:345	disulfide bonds reduction	331:355	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	8	44	theme	antigens	1424:1431	arg1	processing					1386:1395	the processing	1382:1395	the processing of MHC class II-restricted antigens in silver carp	1382:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	3	45	theme	potential	594:602	arg1	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	4	46	theme	other	682:686	arg1	GILTs					688:692	other GILTs	682:692	other GILTs	682:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	3	47	theme	reading	432:438	arg1	frame					440:444	Its open reading frame	423:444	Its open reading frame	423:444	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	1	48	theme	bonds	341:345	arg1	reduction					347:355	disulfide bonds reduction	331:355	disulfide bonds reduction	331:355	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	8	49	theme	important	1364:1372	arg1	role					1374:1377	an important role	1361:1377	an important role	1361:1377	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	3	50	theme	N-linked	604:611	arg1	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	5	51	theme	fish	864:867	arg1	kidney					900:905	head kidney	895:905	head kidney	895:905	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	51	theme	fish	864:867	arg1	organs					876:881	fish immune organs	864:881	fish immune organs	864:881	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	51	theme	fish	864:867	arg1	spleen					884:889	spleen	884:889	spleen	884:889	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	0	52	theme	thiol	82:86	arg1	reductase					88:96	gamma-interferon-inducible lysosomal thiol reductase	45:96	gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix)	45:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	6	53	theme	ScGILT	1042:1047	arg1	protein					1049:1055	ScGILT protein	1042:1055	ScGILT protein	1042:1055	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	0	54	from	reductase	88:96	arg1	Hypophthalmichthys					114:131	Hypophthalmichthys	114:131	Hypophthalmichthys	114:131	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	0	54	from	reductase	88:96	arg1	carp					108:111	silver carp	101:111	silver carp (Hypophthalmichthys molitrix)	101:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	3	55	theme	256	491:493	arg1	acids					501:505	256 amino acids	491:505	256 amino acids	491:505	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	0	56	theme	lysosomal	72:80	arg1	reductase					88:96	gamma-interferon-inducible lysosomal thiol reductase	45:96	gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix)	45:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	6	57	theme	spleen	1008:1013	arg1	cells					1031:1035	spleen and head kidney cells	1008:1035	spleen and head kidney cells	1008:1035	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	1	58	theme	Gamma-interferon-inducible	144:169	arg1	GILT					198:201	GILT	198:201	GILT	198:201	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	1	58	theme	Gamma-interferon-inducible	144:169	arg1	reductase					187:195	Gamma-interferon-inducible lysosomal thiol reductase	144:195	Gamma-interferon-inducible lysosomal thiol reductase (GILT)	144:202	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	4	59	from	arrangement	737:747	arg1	similar					705:711	similar	705:711	similar	705:711	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	59	from	arrangement	737:747	arg1	structures					657:666	The predicted tertiary structures	634:666	The predicted tertiary structures of ScGILT and other GILTs	634:692	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	8	60	from	antigens	1424:1431	arg1	carp					1443:1446	silver carp	1436:1446	silver carp	1436:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	8	61	theme	MHC	1400:1402	arg1	antigens					1424:1431	MHC class II-restricted antigens	1400:1431	MHC class II-restricted antigens in silver carp	1400:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	7	62	theme	His6	1149:1152	arg1	tag					1154:1156	a His6 tag	1147:1156	a His6 tag	1147:1156	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	5	63	theme	ScGILT	768:773	arg1	mRNA					775:778	ScGILT mRNA	768:778	ScGILT mRNA	768:778	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	1	64	theme	lysosomal	171:179	arg1	GILT					198:201	GILT	198:201	GILT	198:201	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	1	64	theme	lysosomal	171:179	arg1	reductase					187:195	Gamma-interferon-inducible lysosomal thiol reductase	144:195	Gamma-interferon-inducible lysosomal thiol reductase (GILT)	144:202	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	4	65	theme	motifs	760:765	arg1	shape					716:720	shape	716:720	shape	716:720	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	4	65	theme	motifs	760:765	arg1	arrangement					737:747	positional arrangement	726:747	positional arrangement	726:747	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	1	66	theme	major	249:253	arg1	complex					274:280	major histocompatibility complex	249:280	major histocompatibility complex (MHC) class II-restricted antigens	249:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	1	66	theme	major	249:253	arg1	MHC					283:285	MHC	283:285	MHC	283:285	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	5	67	from	expression	850:859	arg1	kidney					900:905	head kidney	895:905	head kidney	895:905	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	67	from	expression	850:859	arg1	organs					876:881	fish immune organs	864:881	fish immune organs	864:881	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	5	67	from	expression	850:859	arg1	spleen					884:889	spleen	884:889	spleen	884:889	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	6	68	theme	late	1073:1076	arg1	endosomes					1078:1086	late endosomes	1073:1086	late endosomes	1073:1086	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
29729312	4	69	theme	key	756:758	arg1	motifs					760:765	the key motifs	752:765	the key motifs	752:765	The predicted tertiary structures of ScGILT and other GILTs were quite similar in shape and positional arrangement of the key motifs.					
29729312	1	70	theme	thiol	181:185	arg1	GILT					198:201	GILT	198:201	GILT	198:201	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	1	70	theme	thiol	181:185	arg1	reductase					187:195	Gamma-interferon-inducible lysosomal thiol reductase	144:195	Gamma-interferon-inducible lysosomal thiol reductase (GILT)	144:202	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	8	71	theme	bonds	1324:1328	arg1	reduction					1330:1338	disulfide bonds reduction	1314:1338	disulfide bonds reduction	1314:1338	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	1	72	theme	histocompatibility	255:272	arg1	complex					274:280	major histocompatibility complex	249:280	major histocompatibility complex (MHC) class II-restricted antigens	249:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	1	72	theme	histocompatibility	255:272	arg1	MHC					283:285	MHC	283:285	MHC	283:285	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	8	73	from	carp	1443:1446	arg1	processing					1386:1395	the processing	1382:1395	the processing of MHC class II-restricted antigens in silver carp	1382:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	2	74	theme	GILT	368:371	arg1	ScGILT					382:387	ScGILT	382:387	ScGILT	382:387	Herein, a GILT homolog (ScGILT) was identified from silver carp.					
29729312	2	74	theme	GILT	368:371	arg1	homolog					373:379	a GILT homolog	366:379	a GILT homolog (ScGILT)	366:388	Herein, a GILT homolog (ScGILT) was identified from silver carp.					
29729312	3	75	theme	glycosylation	613:625	arg1	sites					627:631	two potential N-linked glycosylation sites	590:631	two potential N-linked glycosylation sites	590:631	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	5	76	theme	high-level	839:848	arg1	expression					850:859	high-level expression	839:859	high-level expression in fish immune organs, spleen and head kidney	839:905	ScGILT mRNA was constitutively expressed in all detected tissues, with high-level expression in fish immune organs, spleen and head kidney.					
29729312	3	77	theme	base	457:460	arg1	pairs					462:466	771 base pairs	453:466	771 base pairs	453:466	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	1	78	theme	complex	274:280	arg1	antigens					308:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	major histocompatibility complex (MHC) class II-restricted antigens	249:315	Gamma-interferon-inducible lysosomal thiol reductase (GILT) plays an important role in the processing of major histocompatibility complex (MHC) class II-restricted antigens by catalyzing disulfide bonds reduction.					
29729312	0	79	theme	reductase	88:96	arg1	characterization					25:40	Molecular and biological characterization	0:40	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).	0:142	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	0	80	from	characterization	25:40	arg1	Hypophthalmichthys					114:131	Hypophthalmichthys	114:131	Hypophthalmichthys	114:131	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	0	80	from	characterization	25:40	arg1	carp					108:111	silver carp	101:111	silver carp (Hypophthalmichthys molitrix)	101:141	Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix).					
29729312	8	81	theme	class	1404:1408	arg1	antigens					1424:1431	MHC class II-restricted antigens	1400:1431	MHC class II-restricted antigens in silver carp	1400:1446	These results suggested that ScGILT was capable of catalyzing disulfide bonds reduction, and then might play an important role in the processing of MHC class II-restricted antigens in silver carp.					
29729312	3	82	theme	GILT	522:525	arg1	sequence					537:544	GILT signature sequence CQHGX2ECX2NX4C	522:559	GILT signature sequence CQHGX2ECX2NX4C	522:559	Its open reading frame covers 771 base pairs, encoding a protein of 256 amino acids that possesses GILT signature sequence CQHGX2ECX2NX4C, active-site CXXC motif, and two potential N-linked glycosylation sites.					
29729312	7	83	theme	IgG	1237:1239	arg1	bonds					1228:1232	the interchain disulfide bonds	1203:1232	the interchain disulfide bonds of IgG at pH 4.5	1203:1249	Recombinant ScGILT fused with a His6 tag was expressed and purified, and could reduce the interchain disulfide bonds of IgG at pH 4.5.					
29729312	6	84	theme	kidney	1024:1029	arg1	cells					1031:1035	spleen and head kidney cells	1008:1035	spleen and head kidney cells	1008:1035	After stimulation with lipopolysaccharide, the expression of ScGILT mRNA significantly increased in spleen and head kidney cells, and ScGILT protein translocated to late endosomes and lysosomes in HeLa cells.					
30994122	1	0	theme	chemical	156:163	arg1	biology					165:171	chemical biology	156:171	chemical biology	156:171	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	0	1	theme	block	79:83	arg1	synthesis					10:18	Efficient synthesis	0:18	Efficient synthesis	0:18	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	0	1	theme	block	79:83	arg1	extension					34:42	enzymatic extension	24:42	enzymatic extension	24:42	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	3	2	theme	phosphine-mediated	488:505	arg1	reduction					513:521	phosphine-mediated azide reduction	488:521	phosphine-mediated azide reduction	488:521	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	4	3	theme	varying	774:780	arg1	size					782:785	varying size	774:785	varying size	774:785	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	2	4	theme	practical	281:289	arg1	synthesis					291:299	a practical synthesis	279:299	a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS)	279:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	3	5	theme	resulting	532:540	arg1	amine					551:555	the resulting glycosyl amine	528:555	the resulting glycosyl amine	528:555	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	1	6	from	tool	148:151	arg1	biology					165:171	chemical biology	156:171	chemical biology	156:171	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	2	7	theme	building	330:337	arg1	OH					324:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	7	theme	building	330:337	arg1	block					339:343	a building block	328:343	a building block for solid phase peptide synthesis (SPPS)	328:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	8	theme	N-Ac3GlcNAz	312:322	arg1	OH					324:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	8	theme	N-Ac3GlcNAz	312:322	arg1	block					339:343	a building block	328:343	a building block for solid phase peptide synthesis (SPPS)	328:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	5	9	theme	added	875:879	arg1	bulk					888:891	The added steric bulk	871:891	The added steric bulk resulting from incorporation of the azide	871:933	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	3	10	theme	Protecting	387:396	arg1	manipulations					404:416	Protecting group manipulations	387:416	Protecting group manipulations	387:416	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	2	11	theme	peptide	361:367	arg1	SPPS					380:383	SPPS	380:383	SPPS	380:383	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	11	theme	peptide	361:367	arg1	synthesis					369:377	solid phase peptide synthesis	349:377	solid phase peptide synthesis (SPPS)	349:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	5	12	theme	extension	1006:1014	arg1	yield					980:984	the yield	976:984	the yield of enzymatic glycan extension	976:1014	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	4	13	theme	enzymatic	721:729	arg1	extension					738:746	enzymatic glycan extension	721:746	enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases)	721:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	2	14	theme	phase	355:359	arg1	SPPS					380:383	SPPS	380:383	SPPS	380:383	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	14	theme	phase	355:359	arg1	synthesis					369:377	solid phase peptide synthesis	349:377	solid phase peptide synthesis (SPPS)	349:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	0	15	theme	Efficient	0:8	arg1	synthesis					10:18	Efficient synthesis	0:18	Efficient synthesis	0:18	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	2	16	theme	FmocAsn	304:310	arg1	OH					324:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	FmocAsn(N-Ac3GlcNAz)OH	304:325	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	16	theme	FmocAsn	304:310	arg1	block					339:343	a building block	328:343	a building block for solid phase peptide synthesis (SPPS)	328:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	4	17	theme	sugar	754:758	arg1	oxazolines					760:769	sugar oxazolines	754:769	sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases)	754:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	2	18	theme	solid	349:353	arg1	SPPS					380:383	SPPS	380:383	SPPS	380:383	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	2	18	theme	solid	349:353	arg1	synthesis					369:377	solid phase peptide synthesis	349:377	solid phase peptide synthesis (SPPS)	349:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	0	19	theme	enzymatic	24:32	arg1	extension					34:42	enzymatic extension	24:42	enzymatic extension	24:42	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	4	20	with	oxazolines	760:769	arg1	ENGases					861:867	ENGases	861:867	ENGases	861:867	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	4	20	with	oxazolines	760:769	arg1	endo-β-N-acetylglucosaminidases					828:858	several endo-β-N-acetylglucosaminidases	820:858	several endo-β-N-acetylglucosaminidases (ENGases)	820:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	3	21	theme	glycosyl	542:549	arg1	amine					551:555	the resulting glycosyl amine	528:555	the resulting glycosyl amine	528:555	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	5	22	theme	a	958:958	arg1	impact					966:971	no or a minor impact	952:971	impact	966:971	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	5	23	theme	azide	929:933	arg1	incorporation					908:920	incorporation	908:920	incorporation of the azide	908:933	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	4	24	theme	glycan	731:736	arg1	extension					738:746	enzymatic glycan extension	721:746	enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases)	721:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	5	25	contain	have	947:950	arg2	impact					966:971	no or a minor impact	952:971	impact	966:971	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	5	25	contain	have	947:950	arg1	bulk					888:891	The added steric bulk	871:891	The added steric bulk resulting from incorporation of the azide	871:933	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	4	26	theme	block	677:681	arg1	application					649:659	potential application	639:659	potential application of the building block	639:681	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	3	27	theme	chemoselectivity	468:483	arg1	advantage					442:450	advantage	442:450	advantage of the inherent chemoselectivity of phosphine-mediated azide reduction	442:521	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	5	28	theme	glycan	999:1004	arg1	extension					1006:1014	enzymatic glycan extension	989:1014	enzymatic glycan extension	989:1014	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	3	29	theme	group	398:402	arg1	manipulations					404:416	Protecting group manipulations	387:416	Protecting group manipulations	387:416	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	1	30	theme	stable	205:210	arg1	azide					180:184	the azide	176:184	the azide	176:184	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	1	30	theme	stable	205:210	arg1	handle					227:232	a metabolically stable yet accessible handle	189:232	a metabolically stable yet accessible handle within biological systems	189:258	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	5	31	theme	steric	881:886	arg1	bulk					888:891	The added steric bulk	871:891	The added steric bulk resulting from incorporation of the azide	871:933	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	3	32	theme	anhydride	620:628	arg1	opening					585:591	the opening	581:591	the opening of Fmoc protected aspartic anhydride	581:628	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	4	33	theme	significance	802:813	arg1	oxazolines					760:769	sugar oxazolines	754:769	sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases)	754:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	0	34	theme	N-GlcNAz	50:57	arg1	block					79:83	an N-GlcNAz asparagine building block	47:83	an N-GlcNAz asparagine building block	47:83	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	5	35	theme	minor	960:964	arg1	impact					966:971	no or a minor impact	952:971	impact	966:971	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	3	36	theme	inherent	459:466	arg1	chemoselectivity					468:483	the inherent chemoselectivity	455:483	the inherent chemoselectivity of phosphine-mediated azide reduction	455:521	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	4	37	theme	several	820:826	arg1	ENGases					861:867	ENGases	861:867	ENGases	861:867	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	4	37	theme	several	820:826	arg1	endo-β-N-acetylglucosaminidases					828:858	several endo-β-N-acetylglucosaminidases	820:858	several endo-β-N-acetylglucosaminidases (ENGases)	820:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	2	38	theme	OH	324:325	arg1	synthesis					291:299	a practical synthesis	279:299	a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS)	279:384	Herein, we report a practical synthesis of FmocAsn(N-Ac3GlcNAz)OH, a building block for solid phase peptide synthesis (SPPS).					
30994122	5	39	theme	enzymatic	989:997	arg1	extension					1006:1014	enzymatic glycan extension	989:1014	enzymatic glycan extension	989:1014	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	4	40	theme	size	782:785	arg1	oxazolines					760:769	sugar oxazolines	754:769	sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases)	754:868	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	3	41	theme	protected	601:609	arg1	anhydride					620:628	Fmoc protected aspartic anhydride	596:628	Fmoc protected aspartic anhydride	596:628	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	4	42	theme	building	668:675	arg1	block					677:681	the building block	664:681	the building block	664:681	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	5	43	theme	no	952:953	arg1	impact					966:971	no or a minor impact	952:971	impact	966:971	The added steric bulk resulting from incorporation of the azide is shown to have no or a minor impact on the yield of enzymatic glycan extension.					
30994122	1	44	theme	accessible	216:225	arg1	azide					180:184	the azide	176:184	the azide	176:184	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	1	44	theme	accessible	216:225	arg1	handle					227:232	a metabolically stable yet accessible handle	189:232	a metabolically stable yet accessible handle within biological systems	189:258	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	3	45	theme	aspartic	611:618	arg1	anhydride					620:628	Fmoc protected aspartic anhydride	596:628	Fmoc protected aspartic anhydride	596:628	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	1	46	theme	useful	141:146	arg1	N-Azidoacetyl-d-glucosamine					86:112	N-Azidoacetyl-d-glucosamine	86:112	N-Azidoacetyl-d-glucosamine (GlcNAz)	86:121	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	1	46	theme	useful	141:146	arg1	tool					148:151	a particularly useful tool	126:151	a particularly useful tool in chemical biology	126:171	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30994122	0	47	theme	building	70:77	arg1	block					79:83	an N-GlcNAz asparagine building block	47:83	an N-GlcNAz asparagine building block	47:83	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	4	48	theme	biological	791:800	arg1	significance					802:813	biological significance	791:813	biological significance	791:813	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	3	49	theme	azide	507:511	arg1	reduction					513:521	phosphine-mediated azide reduction	488:521	phosphine-mediated azide reduction	488:521	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	4	50	theme	potential	639:647	arg1	application					649:659	potential application	639:659	potential application of the building block	639:681	We show potential application of the building block by establishing it as a substrate for enzymatic glycan extension using sugar oxazolines of varying size and biological significance with several endo-β-N-acetylglucosaminidases (ENGases).					
30994122	0	51	theme	asparagine	59:68	arg1	block					79:83	an N-GlcNAz asparagine building block	47:83	an N-GlcNAz asparagine building block	47:83	Efficient synthesis and enzymatic extension of an N-GlcNAz asparagine building block.					
30994122	3	52	theme	Fmoc	596:599	arg1	anhydride					620:628	Fmoc protected aspartic anhydride	596:628	Fmoc protected aspartic anhydride	596:628	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	3	53	theme	reduction	513:521	arg1	chemoselectivity					468:483	the inherent chemoselectivity	455:483	the inherent chemoselectivity of phosphine-mediated azide reduction	455:521	Protecting group manipulations are minimised by taking advantage of the inherent chemoselectivity of phosphine-mediated azide reduction, and the resulting glycosyl amine is employed directly in the opening of Fmoc protected aspartic anhydride.					
30994122	1	54	theme	biological	241:250	arg1	systems					252:258	biological systems	241:258	biological systems	241:258	N-Azidoacetyl-d-glucosamine (GlcNAz) is a particularly useful tool in chemical biology as the azide is a metabolically stable yet accessible handle within biological systems.					
30467689	4	0	located	present	811:817	arg1	cases					781:785	all cases	777:785	all cases	777:785	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	0	located	present	811:817	arg2	present					811:817	present	811:817	present	811:817	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	0	located	present	811:817	arg2	moiety					800:805	an L-fucose moiety	788:805	an L-fucose moiety	788:805	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	2	1	with	prebiotics	478:487	arg1	β-D-galactosidase					523:539	β-D-galactosidase	523:539	β-D-galactosidase	523:539	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	1	with	prebiotics	478:487	arg1	α-D-glucosidase					642:656	α-D-glucosidase	642:656	α-D-glucosidase	642:656	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	5	2	theme	High	891:894	arg1	concentrations					896:909	High concentrations	891:909	High concentrations of L-fucose as the acceptor substrate	891:947	High concentrations of L-fucose as the acceptor substrate enhanced the synthesis of the oligosaccharides in all cases.					
30467689	2	3	theme	donor	429:433	arg1	substrate					435:443	a donor substrate	427:443	a donor substrate	427:443	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	3	theme	donor	429:433	arg1	D-lactose					414:422	D-lactose	414:422	D-lactose	414:422	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	3	theme	donor	429:433	arg1	two					446:448	two	446:448	two	446:448	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	4	from	cerevisiae	677:686	arg1	prebiotics					478:487	prebiotics	478:487	prebiotics	478:487	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	4	from	cerevisiae	677:686	arg1	β-D-galactosyl-L-fucose					494:516	one β-D-galactosyl-L-fucose	490:516	one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor	490:600	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	4	from	cerevisiae	677:686	arg1	α-D-glucosyl-L-fucosides					612:635	four α-D-glucosyl-L-fucosides	607:635	four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	607:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	5	theme	donor	709:713	arg1	substrate					715:723	a donor substrate	707:723	a donor substrate	707:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	5	theme	donor	709:713	arg1	D-maltose					694:702	D-maltose	694:702	D-maltose	694:702	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	5	6	theme	L-fucose	914:921	arg1	concentrations					896:909	High concentrations	891:909	High concentrations of L-fucose as the acceptor substrate	891:947	High concentrations of L-fucose as the acceptor substrate enhanced the synthesis of the oligosaccharides in all cases.					
30467689	2	7	from	oryzae	558:563	arg1	prebiotics					478:487	prebiotics	478:487	prebiotics	478:487	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	7	from	oryzae	558:563	arg1	β-D-galactosyl-L-fucose					494:516	one β-D-galactosyl-L-fucose	490:516	one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor	490:600	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	7	from	oryzae	558:563	arg1	α-D-glucosyl-L-fucosides					612:635	four α-D-glucosyl-L-fucosides	607:635	four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	607:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	4	8	theme	mass	872:875	arg1	spectrometry					877:888	mass spectrometry	872:888	mass spectrometry	872:888	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	5	9	theme	oligosaccharides	979:994	arg1	synthesis					962:970	the synthesis	958:970	the synthesis of the oligosaccharides in all cases	958:1007	High concentrations of L-fucose as the acceptor substrate enhanced the synthesis of the oligosaccharides in all cases.					
30467689	6	10	from	oryzae	1205:1210	arg1	β-D-galactosidase					1170:1186	β-D-galactosidase	1170:1186	β-D-galactosidase from Aspergillus oryzae	1170:1210	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	1	11	theme	different	211:219	arg1	substrates					227:236	different donor substrates	211:236	different donor substrates	211:236	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	11	theme	different	211:219	arg1	substrate					267:275	the acceptor substrate	254:275	the acceptor substrate	254:275	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	11	theme	different	211:219	arg1	L-fucose					242:249	L-fucose	242:249	L-fucose	242:249	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	6	12	theme	acceptor	1114:1121	arg1	L-fucose					1089:1096	L-fucose	1089:1096	L-fucose	1089:1096	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	6	12	theme	acceptor	1114:1121	arg1	substrate					1123:1131	the acceptor substrate	1110:1131	the acceptor substrate	1110:1131	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	4	13	from	cases	781:785	arg1	present					811:817	present	811:817	present	811:817	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	13	from	cases	781:785	arg1	moiety					800:805	an L-fucose moiety	788:805	an L-fucose moiety	788:805	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	1	14	theme	donor	221:225	arg1	substrates					227:236	different donor substrates	211:236	different donor substrates	211:236	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	14	theme	donor	221:225	arg1	substrate					267:275	the acceptor substrate	254:275	the acceptor substrate	254:275	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	14	theme	donor	221:225	arg1	L-fucose					242:249	L-fucose	242:249	L-fucose	242:249	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	4	15	attach	present	811:817	arg1	cases					781:785	all cases	777:785	all cases	777:785	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	15	attach	present	811:817	arg2	present					811:817	present	811:817	present	811:817	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	15	attach	present	811:817	arg2	moiety					800:805	an L-fucose moiety	788:805	an L-fucose moiety	788:805	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	1	16	theme	various	111:117	arg1	origins					119:125	various origins	111:125	various origins	111:125	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	5	17	theme	acceptor	930:937	arg1	substrate					939:947	the acceptor substrate	926:947	the acceptor substrate	926:947	High concentrations of L-fucose as the acceptor substrate enhanced the synthesis of the oligosaccharides in all cases.					
30467689	2	18	with	α-D-glucosyl-L-fucosides	612:635	arg1	β-D-galactosidase					523:539	β-D-galactosidase	523:539	β-D-galactosidase	523:539	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	18	with	α-D-glucosyl-L-fucosides	612:635	arg1	α-D-glucosidase					642:656	α-D-glucosidase	642:656	α-D-glucosidase	642:656	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	19	theme	CloneZyme	387:395	arg1	Gly-001-02					397:406	glycosidase CloneZyme Gly-001-02	375:406	glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	375:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	20	dep	synthesized	313:323	arg1	β-D-galactosyl-L-fucosides					343:368	three β-D-galactosyl-L-fucosides	337:368	three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	337:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	0	21	theme	Disaccharides	31:43	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.	0:87	Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.					
30467689	2	22	theme	glycosidase	375:385	arg1	Gly-001-02					397:406	glycosidase CloneZyme Gly-001-02	375:406	glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	375:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	1	23	theme	origins	119:125	arg1	Glycosylhydrolases					89:106	Glycosylhydrolases	89:106	Glycosylhydrolases of various origins	89:125	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	2	24	theme	different	284:292	arg1	follows					328:334	follows	328:334	follows	328:334	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	24	theme	different	284:292	arg1	disaccharides					294:306	Eight different disaccharides	278:306	Eight different disaccharides	278:306	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	25	theme	similar	467:473	arg1	structure					457:465	a structure	455:465	a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	455:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	6	26	theme	highest	1142:1148	arg1	yield					1150:1154	the highest yield	1138:1154	the highest yield	1138:1154	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	6	26	theme	highest	1142:1148	arg1	%					1162:1162	20%	1160:1162	20%	1160:1162	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	1	27	theme	acceptor	258:265	arg1	substrates					227:236	different donor substrates	211:236	different donor substrates	211:236	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	27	theme	acceptor	258:265	arg1	substrate					267:275	the acceptor substrate	254:275	the acceptor substrate	254:275	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	1	27	theme	acceptor	258:265	arg1	L-fucose					242:249	L-fucose	242:249	L-fucose	242:249	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	0	28	theme	Various	72:78	arg1	Origins					80:86	Various Origins	72:86	Various Origins	72:86	Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.					
30467689	0	29	from	Origins	80:86	arg1	Glycosylhydrolases					48:65	Glycosylhydrolases	48:65	Glycosylhydrolases from Various Origins	48:86	Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.					
30467689	2	30	with	β-D-galactosyl-L-fucose	494:516	arg1	β-D-galactosidase					523:539	β-D-galactosidase	523:539	β-D-galactosidase	523:539	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	30	with	β-D-galactosyl-L-fucose	494:516	arg1	α-D-glucosidase					642:656	α-D-glucosidase	642:656	α-D-glucosidase	642:656	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	1	31	used	used	132:135	arg2	Glycosylhydrolases					89:106	Glycosylhydrolases	89:106	Glycosylhydrolases of various origins	89:125	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	6	32	used	used	1102:1105	arg2	L-fucose					1089:1096	L-fucose	1089:1096	L-fucose	1089:1096	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	6	32	used	used	1102:1105	arg2	substrate					1123:1131	the acceptor substrate	1110:1131	the acceptor substrate	1110:1131	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	6	33	theme	fucose-containing	1052:1068	arg1	disaccharides					1070:1082	fucose-containing disaccharides	1052:1082	fucose-containing disaccharides	1052:1082	The three enzymes were able to synthesize fucose-containing disaccharides when L-fucose was used as the acceptor substrate, and the highest yield was 20% using β-D-galactosidase from Aspergillus oryzae.					
30467689	5	34	from	synthesis	962:970	arg1	cases					1003:1007	all cases	999:1007	all cases	999:1007	High concentrations of L-fucose as the acceptor substrate enhanced the synthesis of the oligosaccharides in all cases.					
30467689	1	35	theme	fucose-containing	148:164	arg1	disaccharides					166:178	fucose-containing disaccharides	148:178	fucose-containing disaccharides	148:178	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	4	36	theme	L-fucose	791:798	arg1	present					811:817	present	811:817	present	811:817	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	4	36	theme	L-fucose	791:798	arg1	moiety					800:805	an L-fucose moiety	788:805	an L-fucose moiety	788:805	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
30467689	2	37	with	β-D-galactosyl-L-fucosides	343:368	arg1	Gly-001-02					397:406	glycosidase CloneZyme Gly-001-02	375:406	glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate	375:723	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	38	theme	substrate	586:594	arg1	donor					596:600	a substrate donor	584:600	a substrate donor	584:600	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	2	38	theme	substrate	586:594	arg1	D-lactose					571:579	D-lactose	571:579	D-lactose	571:579	Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate.					
30467689	0	39	theme	Fucose-Containing	13:29	arg1	Disaccharides					31:43	Fucose-Containing Disaccharides	13:43	Fucose-Containing Disaccharides	13:43	Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.					
30467689	1	40	theme	prebiotic	185:193	arg1	potential					195:203	prebiotic potential	185:203	prebiotic potential using different donor substrates and L-fucose as the acceptor substrate	185:275	Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate.					
30467689	4	41	from	present	811:817	arg1	cases					781:785	all cases	777:785	all cases	777:785	In all cases, an L-fucose moiety was present, and it was confirmed for β-D-galactosyl-L-fucose by mass spectrometry.					
31655584	0	0	theme	plantarum	89:97	arg1	WCFS1					99:103	Lactobacillus plantarum WCFS1	75:103	Lactobacillus plantarum WCFS1	75:103	Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.					
31655584	3	1	theme	plantarum	334:342	arg1	WCFS1					344:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1 possesses eleven proteins included in GH13 family.					
31655584	3	2	contain	possesses	350:358	arg1	WCFS1					344:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1 possesses eleven proteins included in GH13 family.					
31655584	3	2	contain	possesses	350:358	arg2	proteins					367:374	eleven proteins	360:374	eleven proteins included in GH13 family	360:398	Lactobacillus plantarum WCFS1 possesses eleven proteins included in GH13 family.					
31655584	1	3	theme	internal	172:179	arg1	linkages					199:206	the internal α-1, 4-glucosidic linkages	168:206	the internal α-1, 4-glucosidic linkages of starch	168:216	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	8	4	theme	high	865:868	arg1	activity					881:888	high hydrolytic activity	865:888	high hydrolytic activity	865:888	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	0	5	theme	Lactobacillus	75:87	arg1	WCFS1					99:103	Lactobacillus plantarum WCFS1	75:103	Lactobacillus plantarum WCFS1	75:103	Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.					
31655584	1	6	theme	α-1	181:183	arg1	linkages					199:206	the internal α-1, 4-glucosidic linkages	168:206	the internal α-1, 4-glucosidic linkages of starch	168:216	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	6	7	theme	structural	658:667	arg1	features					669:676	structural features	658:676	structural features typical of GH13_20 subfamily which were absent in Lp_0179	658:734	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31655584	11	8	theme	substrate	1351:1359	arg1	range					1361:1365	The substrate range	1347:1365	The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase	1347:1445	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	9	from	present	1553:1559	arg1	number					1572:1577	a broad number	1564:1577	a broad number of starch-carbohydrates	1564:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	9	10	theme	exotype	1118:1124	arg1	hydrolase					1133:1141	an exotype glucan hydrolase	1115:1141	an exotype glucan hydrolase	1115:1141	In relation to the hydrolysis of starch-related carbohydrates, Lp_0179 only hydrolysed maltopentaose and dextrin, demonstrating that is an exotype glucan hydrolase.					
31655584	10	11	theme	great	1263:1267	arg1	preference					1269:1278	a great preference	1261:1278	a great preference towards α-1,4-linkages typical of maltogenic amylases	1261:1332	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	11	12	theme	glycosidic	1533:1542	arg1	linkages					1544:1551	α-1,4 glycosidic linkages	1527:1551	α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates	1527:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	9	13	theme	glucan	1126:1131	arg1	hydrolase					1133:1141	an exotype glucan hydrolase	1115:1141	an exotype glucan hydrolase	1115:1141	In relation to the hydrolysis of starch-related carbohydrates, Lp_0179 only hydrolysed maltopentaose and dextrin, demonstrating that is an exotype glucan hydrolase.					
31655584	11	14	dep	CONCLUSIONS	1335:1345	arg1	suggest					1447:1453	suggest	1447:1453	suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions	1447:1720	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	15	theme	biochemical	1382:1392	arg1	properties					1394:1403	the biochemical properties	1378:1403	The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase	1347:1445	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	6	16	from	Lp_0179	728:734	arg1	absent					718:723	absent	718:723	absent	718:723	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31655584	10	17	theme	typical	1303:1309	arg1	α-1,4-linkages					1288:1301	α-1,4-linkages	1288:1301	α-1,4-linkages typical of maltogenic amylases	1288:1332	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	11	18	theme	promising	1488:1496	arg1	enzyme					1465:1470	this enzyme	1460:1470	this enzyme	1460:1470	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	18	theme	promising	1488:1496	arg1	enzyme					1498:1503	a very promising enzyme	1481:1503	a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity	1481:1682	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	7	19	dep	Escherichia	812:822	arg1	coli					824:827	coli	824:827	coli	824:827	Genes encoding Lp_0179 (Amy2) and Lp_2757 were cloned and overexpressed in Escherichia coli BL21(DE3).					
31655584	8	20	theme	Purified	840:847	arg1	proteins					849:856	Purified proteins	840:856	Purified proteins	840:856	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	11	21	theme	hypothetical	1643:1654	arg1	activity					1675:1682	an hypothetical transglucosylation activity	1640:1682	an hypothetical transglucosylation activity	1640:1682	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	2	22	theme	α-amylase	266:274	arg1	family					244:249	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	22	theme	α-amylase	266:274	arg1	family					276:281	the main α-amylase family	257:281	the main α-amylase family in the carbohydrate-active database	257:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	10	23	theme	maltogenic	1314:1323	arg1	amylases					1325:1332	maltogenic amylases	1314:1332	maltogenic amylases	1314:1332	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	11	24	theme	Lp_2757	1418:1424	arg1	α-amylase					1437:1445	Lp_2757 maltogenic α-amylase	1418:1445	Lp_2757 maltogenic α-amylase	1418:1445	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	25	theme	transglucosylation	1656:1673	arg1	activity					1675:1682	an hypothetical transglucosylation activity	1640:1682	an hypothetical transglucosylation activity	1640:1682	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	6	26	from	absent	718:723	arg1	Lp_0179					728:734	Lp_0179	728:734	Lp_0179	728:734	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31655584	1	27	theme	linkages	199:206	arg1	hydrolysis					154:163	the hydrolysis	150:163	the hydrolysis of the internal α-1, 4-glucosidic linkages of starch	150:216	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	9	28	theme	starch-related	1012:1025	arg1	carbohydrates					1027:1039	starch-related carbohydrates	1012:1039	starch-related carbohydrates	1012:1039	In relation to the hydrolysis of starch-related carbohydrates, Lp_0179 only hydrolysed maltopentaose and dextrin, demonstrating that is an exotype glucan hydrolase.					
31655584	11	29	theme	maltogenic	1426:1435	arg1	α-amylase					1437:1445	Lp_2757 maltogenic α-amylase	1418:1445	Lp_2757 maltogenic α-amylase	1418:1445	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	1	30	dep	α-1	181:183	arg1	4-glucosidic					186:197	4-glucosidic	186:197	4-glucosidic	186:197	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	11	31	theme	present	1553:1559	arg1	linkages					1544:1551	α-1,4 glycosidic linkages	1527:1551	α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates	1527:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	9	32	theme	carbohydrates	1027:1039	arg1	hydrolysis					998:1007	the hydrolysis	994:1007	the hydrolysis of starch-related carbohydrates	994:1039	In relation to the hydrolysis of starch-related carbohydrates, Lp_0179 only hydrolysed maltopentaose and dextrin, demonstrating that is an exotype glucan hydrolase.					
31655584	11	33	from	number	1572:1577	arg1	present					1553:1559	present	1553:1559	present	1553:1559	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	1	34	theme	BACKGROUND	106:115	arg1	α-Amylases					117:126	BACKGROUND α-Amylases	106:126	BACKGROUND α-Amylases	106:126	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	5	35	dep	Lp_0179	545:551	arg1	α-amylases					578:587	L. plantarum α-amylases	565:587	L. plantarum α-amylases	565:587	RESULTS In this study, Lp_0179 and Lp_2757 L. plantarum α-amylases were structurally and biochemically characterized.					
31655584	1	36	theme	starch	211:216	arg1	linkages					199:206	the internal α-1, 4-glucosidic linkages	168:206	the internal α-1, 4-glucosidic linkages of starch	168:216	BACKGROUND α-Amylases specifically catalyse the hydrolysis of the internal α-1, 4-glucosidic linkages of starch.					
31655584	11	37	theme	activity	1675:1682	arg1	investigation					1623:1635	the investigation	1619:1635	the investigation of an hypothetical transglucosylation activity	1619:1682	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	38	theme	appropriate	1690:1700	arg1	conditions					1711:1720	appropriate reaction conditions	1690:1720	appropriate reaction conditions	1690:1720	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	2	39	theme	carbohydrate-active	290:308	arg1	database					310:317	the carbohydrate-active database	286:317	the carbohydrate-active database	286:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	8	40	theme	Lp_0179	952:958	arg1	efficiency					938:947	the catalytic efficiency	924:947	the catalytic efficiency of Lp_0179 remarkably higher	924:976	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	0	41	theme	hydrolase	39:47	arg1	family					49:54	glycoside hydrolase family 13	29:57	glycoside hydrolase family 13	29:57	Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.					
31655584	11	42	theme	α-1,4	1527:1531	arg1	linkages					1544:1551	α-1,4 glycosidic linkages	1527:1551	α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates	1527:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	43	attach	present	1553:1559	arg1	number					1572:1577	a broad number	1564:1577	a broad number of starch-carbohydrates	1564:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	43	attach	present	1553:1559	arg2	linkages					1544:1551	α-1,4 glycosidic linkages	1527:1551	α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates	1527:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	44	theme	linkages	1544:1551	arg1	hydrolysis					1513:1522	the hydrolysis	1509:1522	the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates	1509:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	0	45	theme	glycoside	29:37	arg1	family					49:54	glycoside hydrolase family 13	29:57	glycoside hydrolase family 13	29:57	Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.					
31655584	7	46	theme	Escherichia	812:822	arg1	DE3					834:836	DE3	834:836	DE3	834:836	Genes encoding Lp_0179 (Amy2) and Lp_2757 were cloned and overexpressed in Escherichia coli BL21(DE3).					
31655584	7	46	theme	Escherichia	812:822	arg1	BL21					829:832	Escherichia coli BL21	812:832	Escherichia coli BL21(DE3)	812:837	Genes encoding Lp_0179 (Amy2) and Lp_2757 were cloned and overexpressed in Escherichia coli BL21(DE3).					
31655584	5	47	dep	RESULTS	522:528	arg1	characterized					625:637	characterized	625:637	were structurally and biochemically characterized	589:637	RESULTS In this study, Lp_0179 and Lp_2757 L. plantarum α-amylases were structurally and biochemically characterized.					
31655584	10	48	theme	non-cyclic	1212:1221	arg1	oligo-					1223:1228	other non-cyclic oligo-	1206:1228	other non-cyclic oligo-	1206:1228	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	4	49	theme	maltogenic	476:485	arg1	Lp_2757					498:504	Lp_2757	498:504	Lp_2757	498:504	Among these, proteins annotated as maltose-forming α-amylase (Lp_0179) and maltogenic α-amylase (Lp_2757) were included.					
31655584	4	49	theme	maltogenic	476:485	arg1	α-amylase					487:495	maltogenic α-amylase	476:495	maltogenic α-amylase (Lp_2757)	476:505	Among these, proteins annotated as maltose-forming α-amylase (Lp_0179) and maltogenic α-amylase (Lp_2757) were included.					
31655584	2	50	theme	GH	240:241	arg1	family					244:249	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	50	theme	GH	240:241	arg1	family					276:281	the main α-amylase family	257:281	the main α-amylase family in the carbohydrate-active database	257:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	11	51	theme	reaction	1702:1709	arg1	conditions					1711:1720	appropriate reaction conditions	1690:1720	appropriate reaction conditions	1690:1720	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	11	52	theme	starch-carbohydrates	1582:1601	arg1	number					1572:1577	a broad number	1564:1577	a broad number of starch-carbohydrates	1564:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	0	53	theme	family	49:54	arg1	diversity					16:24	the diversity	12:24	the diversity of glycoside hydrolase family 13	12:57	Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.					
31655584	10	54	theme	other	1206:1210	arg1	oligo-					1223:1228	other non-cyclic oligo-	1206:1228	other non-cyclic oligo-	1206:1228	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	3	55	theme	Lactobacillus	320:332	arg1	WCFS1					344:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1	320:348	Lactobacillus plantarum WCFS1 possesses eleven proteins included in GH13 family.					
31655584	2	56	theme	hydrolase	229:237	arg1	family					244:249	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	56	theme	hydrolase	229:237	arg1	family					276:281	the main α-amylase family	257:281	the main α-amylase family in the carbohydrate-active database	257:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	4	57	theme	maltose-forming	436:450	arg1	Lp_0179					463:469	Lp_0179	463:469	Lp_0179	463:469	Among these, proteins annotated as maltose-forming α-amylase (Lp_0179) and maltogenic α-amylase (Lp_2757) were included.					
31655584	4	57	theme	maltose-forming	436:450	arg1	α-amylase					452:460	maltose-forming α-amylase	436:460	maltose-forming α-amylase (Lp_0179)	436:470	Among these, proteins annotated as maltose-forming α-amylase (Lp_0179) and maltogenic α-amylase (Lp_2757) were included.					
31655584	5	58	theme	L.	565:566	arg1	α-amylases					578:587	L. plantarum α-amylases	565:587	L. plantarum α-amylases	565:587	RESULTS In this study, Lp_0179 and Lp_2757 L. plantarum α-amylases were structurally and biochemically characterized.					
31655584	2	59	from	family	276:281	arg1	database					310:317	the carbohydrate-active database	286:317	the carbohydrate-active database	286:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	60	theme	Glycoside	219:227	arg1	family					244:249	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	60	theme	Glycoside	219:227	arg1	family					276:281	the main α-amylase family	257:281	the main α-amylase family in the carbohydrate-active database	257:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	61	theme	main	261:264	arg1	family					244:249	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13	219:252	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	2	61	theme	main	261:264	arg1	family					276:281	the main α-amylase family	257:281	the main α-amylase family in the carbohydrate-active database	257:317	Glycoside hydrolase (GH) family 13 is the main α-amylase family in the carbohydrate-active database.					
31655584	5	62	theme	plantarum	568:576	arg1	α-amylases					578:587	L. plantarum α-amylases	565:587	L. plantarum α-amylases	565:587	RESULTS In this study, Lp_0179 and Lp_2757 L. plantarum α-amylases were structurally and biochemically characterized.					
31655584	6	63	theme	subfamily	697:705	arg1	typical					678:684	typical	678:684	typical	678:684	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31655584	8	64	theme	higher	971:976	arg1	efficiency					938:947	the catalytic efficiency	924:947	the catalytic efficiency of Lp_0179 remarkably higher	924:976	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	8	65	theme	catalytic	928:936	arg1	efficiency					938:947	the catalytic efficiency	924:947	the catalytic efficiency of Lp_0179 remarkably higher	924:976	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	6	66	theme	GH13_20	689:695	arg1	subfamily					697:705	GH13_20 subfamily	689:705	GH13_20 subfamily which were absent in Lp_0179	689:734	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31655584	10	67	theme	amylases	1325:1332	arg1	typical					1303:1309	typical	1303:1309	typical	1303:1309	However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards α-1,4-linkages typical of maltogenic amylases.					
31655584	8	68	theme	hydrolytic	870:879	arg1	activity					881:888	high hydrolytic activity	865:888	high hydrolytic activity	865:888	Purified proteins showed high hydrolytic activity on pNP-α-D-maltopyranoside, being the catalytic efficiency of Lp_0179 remarkably higher.					
31655584	11	69	theme	broad	1566:1570	arg1	number					1572:1577	a broad number	1564:1577	a broad number of starch-carbohydrates	1564:1601	CONCLUSIONS The substrate range as well as the biochemical properties exhibited by Lp_2757 maltogenic α-amylase suggest that this enzyme could be a very promising enzyme for the hydrolysis of α-1,4 glycosidic linkages present in a broad number of starch-carbohydrates, as well as for the investigation of an hypothetical transglucosylation activity under appropriate reaction conditions.					
31655584	6	70	theme	typical	678:684	arg1	features					669:676	structural features	658:676	structural features typical of GH13_20 subfamily which were absent in Lp_0179	658:734	Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179.					
31260765	7	0	contain	have	1022:1025	arg2	application					1037:1047	plausible application	1027:1047	plausible application in food industry	1027:1064	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	7	0	contain	have	1022:1025	arg1	hydrogel					1009:1016	the composite hydrogel	995:1016	the composite hydrogel	995:1016	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	1	1	theme	mechanical	241:250	arg1	properties					252:261	their signature physiochemical and mechanical properties	206:261	their signature physiochemical and mechanical properties	206:261	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	0	2	theme	xanthan	67:73	arg1	hydrogel					55:62	a novel composite hydrogel	37:62	a novel composite hydrogel of xanthan, gellan and pullulan	37:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	5	3	theme	viscoelastic	729:740	arg1	properties					742:751	viscoelastic properties	729:751	viscoelastic properties	729:751	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	7	4	theme	Overall	964:970	arg1	results					972:978	Overall results	964:978	Overall results	964:978	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	6	5	theme	swelling	829:836	arg1	ratio					838:842	swelling ratio	829:842	swelling ratio	829:842	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	4	6	theme	stable	594:599	arg1	gel					601:603	a stable gel	592:603	a stable gel having high water retention ability and swelling ratio	592:658	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	0	7	theme	gellan	76:81	arg1	hydrogel					55:62	a novel composite hydrogel	37:62	a novel composite hydrogel of xanthan, gellan and pullulan	37:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	5	8	theme	synergistic	665:675	arg1	effects					677:683	The synergistic effects	661:683	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour	661:770	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	2	9	theme	polysaccharides	279:293	arg1	Use					264:266	Use	264:266	Use of natural polysaccharides as the components of hydrogel composite	264:333	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	1	10	theme	dimensional	117:127	arg1	structures					139:148	three dimensional polymeric structures	111:148	three dimensional polymeric structures	111:148	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	1	10	theme	dimensional	117:127	arg1	Hydrogels					97:105	Hydrogels	97:105	Hydrogels	97:105	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	5	11	from	effects	677:683	arg1	properties					742:751	viscoelastic properties	729:751	viscoelastic properties	729:751	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	5	11	from	effects	677:683	arg1	behaviour					762:770	flow behaviour	757:770	flow behaviour	757:770	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	7	12	from	application	1037:1047	arg1	industry					1057:1064	food industry	1052:1064	food industry	1052:1064	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	2	13	theme	natural	271:277	arg1	polysaccharides					279:293	natural polysaccharides	271:293	natural polysaccharides	271:293	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	0	14	theme	pullulan	87:94	arg1	hydrogel					55:62	a novel composite hydrogel	37:62	a novel composite hydrogel of xanthan, gellan and pullulan	37:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	6	15	theme	components	909:918	arg1	ionisation					886:895	the ionisation	882:895	the ionisation of hydrogel components	882:918	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	1	16	theme	great	161:165	arg1	importance					167:176	great importance	161:176	great importance	161:176	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	6	17	theme	hydrogel	900:907	arg1	components					909:918	hydrogel components	900:918	hydrogel components	900:918	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	7	18	theme	behaviour	1110:1118	arg1	values					1130:1135	flow behaviour index (n) values	1105:1135	flow behaviour index (n) values for composites within the desirable range	1105:1177	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	7	18	theme	behaviour	1110:1118	arg1	n					1127:1127	n	1127:1127	n	1127:1127	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	6	19	theme	hydrogel	795:802	arg1	composite					804:812	The hydrogel composite	791:812	The hydrogel composite	791:812	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	2	20	theme	hydrogel	316:323	arg1	composite					325:333	hydrogel composite	316:333	hydrogel composite	316:333	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	7	21	theme	viscoelastic	1081:1092	arg1	nature					1094:1099	viscoelastic nature	1081:1099	viscoelastic nature	1081:1099	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	7	22	theme	index	1120:1124	arg1	values					1130:1135	flow behaviour index (n) values	1105:1135	flow behaviour index (n) values for composites within the desirable range	1105:1177	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	7	22	theme	index	1120:1124	arg1	n					1127:1127	n	1127:1127	n	1127:1127	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	2	23	theme	non-toxic	369:377	arg1	nature					379:384	their non-toxic nature	363:384	their non-toxic nature	363:384	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	2	24	theme	viscoelastic	426:437	arg1	properties					439:448	viscoelastic properties	426:448	viscoelastic properties	426:448	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	0	25	theme	rheological	14:24	arg1	studies					26:32	rheological studies	14:32	rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan	14:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	7	26	theme	flow	1105:1108	arg1	values					1130:1135	flow behaviour index (n) values	1105:1135	flow behaviour index (n) values for composites within the desirable range	1105:1177	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	7	26	theme	flow	1105:1108	arg1	n					1127:1127	n	1127:1127	n	1127:1127	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	3	27	theme	novel	479:483	arg1	hydrogel					485:492	a novel hydrogel	477:492	a novel hydrogel composed of xanthan, gellan and pullulan	477:533	The present study reports a novel hydrogel composed of xanthan, gellan and pullulan.					
31260765	7	28	theme	desirable	1163:1171	arg1	range					1173:1177	the desirable range	1159:1177	the desirable range	1159:1177	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	5	29	theme	hydrogel	717:724	arg1	components					699:708	individual components	688:708	individual components of the hydrogel	688:724	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	1	30	theme	due	199:201	arg1	sector					192:197	industrial sector	181:197	industrial sector due to their signature physiochemical and mechanical properties	181:261	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	4	31	theme	water	617:621	arg1	retention					623:631	high water retention ability and swelling ratio	612:658	retention	623:631	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	5	32	theme	flow	757:760	arg1	behaviour					762:770	flow behaviour	757:770	flow behaviour	757:770	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	0	33	theme	composite	45:53	arg1	hydrogel					55:62	a novel composite hydrogel	37:62	a novel composite hydrogel of xanthan, gellan and pullulan	37:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	7	34	theme	food	1052:1055	arg1	industry					1057:1064	food industry	1052:1064	food industry	1052:1064	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	2	35	theme	great	341:345	arg1	interest					347:354	great interest	341:354	great interest	341:354	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	0	36	theme	novel	39:43	arg1	hydrogel					55:62	a novel composite hydrogel	37:62	a novel composite hydrogel of xanthan, gellan and pullulan	37:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	6	37	theme	moieties	954:961	arg1	presence					939:946	the presence	935:946	the presence of OH moieties	935:961	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	4	38	dep	retention	623:631	arg1	ability					633:639	ability	633:639	ability	633:639	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	4	38	dep	retention	623:631	arg1	ratio					654:658	ratio	654:658	ratio	654:658	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	7	39	theme	plausible	1027:1035	arg1	application					1037:1047	plausible application	1027:1047	plausible application in food industry	1027:1064	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	6	40	theme	OH	951:952	arg1	moieties					954:961	OH moieties	951:961	OH moieties	951:961	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	5	41	theme	individual	688:697	arg1	components					699:708	individual components	688:708	individual components of the hydrogel	688:724	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	6	42	dep	presence	939:946	arg1	response					923:930	response	923:930	response	923:930	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	5	43	theme	components	699:708	arg1	effects					677:683	The synergistic effects	661:683	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour	661:770	The synergistic effects of individual components of the hydrogel on viscoelastic properties and flow behaviour were also studied.					
31260765	6	44	theme	alkaline	847:854	arg1	environment					856:866	alkaline environment	847:866	alkaline environment attributed to the ionisation of hydrogel components	847:918	The hydrogel composite also indicated swelling ratio in alkaline environment attributed to the ionisation of hydrogel components in response to the presence of OH moieties.					
31260765	2	45	theme	composite	325:333	arg1	components					302:311	the components	298:311	the components of hydrogel composite	298:333	Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties.					
31260765	1	46	theme	industrial	181:190	arg1	sector					192:197	industrial sector	181:197	industrial sector due to their signature physiochemical and mechanical properties	181:261	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	1	47	theme	polymeric	129:137	arg1	structures					139:148	three dimensional polymeric structures	111:148	three dimensional polymeric structures	111:148	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	1	47	theme	polymeric	129:137	arg1	Hydrogels					97:105	Hydrogels	97:105	Hydrogels	97:105	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	3	48	theme	present	455:461	arg1	study					463:467	The present study	451:467	The present study	451:467	The present study reports a novel hydrogel composed of xanthan, gellan and pullulan.					
31260765	1	49	theme	physiochemical	222:235	arg1	properties					252:261	their signature physiochemical and mechanical properties	206:261	their signature physiochemical and mechanical properties	206:261	Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties.					
31260765	0	50	theme	hydrogel	55:62	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	0	50	theme	hydrogel	55:62	arg1	studies					26:32	rheological studies	14:32	rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan	14:94	Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.					
31260765	4	51	contain	having	605:610	arg2	retention					623:631	high water retention ability and swelling ratio	612:658	retention	623:631	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	4	51	contain	having	605:610	arg2	swelling					645:652	swelling	645:652	swelling	645:652	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	4	51	contain	having	605:610	arg1	gel					601:603	a stable gel	592:603	a stable gel having high water retention ability and swelling ratio	592:658	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
31260765	7	52	theme	composite	999:1007	arg1	hydrogel					1009:1016	the composite hydrogel	995:1016	the composite hydrogel	995:1016	Overall results indicated that the composite hydrogel can have plausible application in food industry as it displays viscoelastic nature and flow behaviour index (n) values for composites within the desirable range.					
31260765	4	53	theme	high	612:615	arg1	retention					623:631	high water retention ability and swelling ratio	612:658	retention	623:631	Unlike, pullulan and xanthan, this tri-composite formed a stable gel having high water retention ability and swelling ratio.					
29628264	6	0	theme	bacterial	1005:1013	arg1	cellulose					1015:1023	bacterial cellulose	1005:1023	bacterial cellulose produced in the presence of wood-based xylan	1005:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	2	1	theme	commercial	397:406	arg1	mixture					418:424	a commercial cellulase mixture	395:424	a commercial cellulase mixture	395:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	2	2	from	effects	336:342	arg1	hydrolysis					379:388	the enzymatic hydrolysis	365:388	the enzymatic hydrolysis with a commercial cellulase mixture	365:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	6	3	theme	enzymatic	981:989	arg1	hydrolysis					991:1000	The reported easier enzymatic hydrolysis	961:1000	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan	961:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	6	4	theme	new	1077:1079	arg1	insights					1081:1088	new insights	1077:1088	new insights	1077:1088	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	3	5	theme	enzymatic	548:556	arg1	hydrolysis					558:567	enzymatic hydrolysis	548:567	enzymatic hydrolysis	548:567	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	6	6	theme	biomass	1102:1108	arg1	recalcitrance					1110:1122	biomass recalcitrance	1102:1122	biomass recalcitrance	1102:1122	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	5	7	theme	small-angle	802:812	arg1	scattering					820:829	small-angle X-ray scattering	802:829	small-angle X-ray scattering	802:829	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	6	8	theme	easier	974:979	arg1	hydrolysis					991:1000	The reported easier enzymatic hydrolysis	961:1000	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan	961:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	3	9	theme	bacterial	593:601	arg1	cellulose					603:611	hemicellulose-free bacterial cellulose	574:611	hemicellulose-free bacterial cellulose	574:611	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	1	10	theme	enzymatic	191:199	arg1	hydrolysis					201:210	enzymatic hydrolysis	191:210	enzymatic hydrolysis	191:210	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	5	11	theme	X-ray	814:818	arg1	scattering					820:829	small-angle X-ray scattering	802:829	small-angle X-ray scattering	802:829	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	6	12	theme	reported	965:972	arg1	hydrolysis					991:1000	The reported easier enzymatic hydrolysis	961:1000	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan	961:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	5	13	used	used	835:838	arg2	scattering					820:829	small-angle X-ray scattering	802:829	small-angle X-ray scattering	802:829	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	1	14	theme	biofuels	99:106	arg1	production					85:94	The production	81:94	The production of biofuels and other chemicals from lignocellulosic biomass	81:155	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	1	15	theme	hydrolysis	201:210	arg1	inefficiency					175:186	the inefficiency	171:186	the inefficiency of enzymatic hydrolysis	171:210	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	5	16	theme	nanoscale	865:873	arg1	morphology					875:884	the average nanoscale morphology	853:884	the average nanoscale morphology of bacterial cellulose	853:907	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	3	17	theme	hemicelluloses	478:491	arg1	presence					466:473	the presence	462:473	the presence of hemicelluloses	462:491	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	0	18	theme	Enzymatic	0:8	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites	0:78	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites.					
29628264	1	19	theme	other	112:116	arg1	chemicals					118:126	other chemicals	112:126	other chemicals	112:126	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	0	20	theme	biomimetic	24:33	arg1	composites					69:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites.					
29628264	6	21	theme	xylan	1064:1068	arg1	presence					1041:1048	the presence	1037:1048	the presence of wood-based xylan	1037:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	6	22	theme	wood-based	1053:1062	arg1	xylan					1064:1068	wood-based xylan	1053:1068	wood-based xylan	1053:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	1	23	from	biomass	149:155	arg1	production					85:94	The production	81:94	The production of biofuels and other chemicals from lignocellulosic biomass	81:155	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	1	24	theme	chemicals	118:126	arg1	production					85:94	The production	81:94	The production of biofuels and other chemicals from lignocellulosic biomass	81:155	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	5	25	theme	cellulose	899:907	arg1	morphology					875:884	the average nanoscale morphology	853:884	the average nanoscale morphology of bacterial cellulose	853:907	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	2	26	theme	hemicelluloses	347:360	arg1	effects					336:342	the effects	332:342	the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture	332:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	4	27	theme	easier	633:638	arg1	hydrolysis					640:649	the easier hydrolysis	629:649	the easier hydrolysis	629:649	The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles.					
29628264	0	28	theme	cellulose-hemicellulose	45:67	arg1	composites					69:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites.					
29628264	2	29	theme	cellulose	274:282	arg1	hemicelluloses					299:312	bacterial cellulose and wood-based hemicelluloses	264:312	bacterial cellulose and wood-based hemicelluloses	264:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	0	30	theme	bacterial	35:43	arg1	composites					69:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	biomimetic bacterial cellulose-hemicellulose composites	24:78	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites.					
29628264	2	31	theme	bacterial	264:272	arg1	hemicelluloses					299:312	bacterial cellulose and wood-based hemicelluloses	264:312	bacterial cellulose and wood-based hemicelluloses	264:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	4	32	theme	substrate	702:710	arg1	structure					685:693	the nanoscale structure	671:693	the nanoscale structure of the substrate	671:710	The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles.					
29628264	5	33	theme	average	857:863	arg1	morphology					875:884	the average nanoscale morphology	853:884	the average nanoscale morphology of bacterial cellulose	853:907	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	2	34	with	hydrolysis	379:388	arg1	mixture					418:424	a commercial cellulase mixture	395:424	a commercial cellulase mixture	395:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	1	35	theme	lignocellulosic	133:147	arg1	biomass					149:155	lignocellulosic biomass	133:155	lignocellulosic biomass	133:155	The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis.					
29628264	0	36	theme	composites	69:78	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites	0:78	Enzymatic hydrolysis of biomimetic bacterial cellulose-hemicellulose composites.					
29628264	2	37	theme	composite	231:239	arg1	material					241:248	a biomimetic composite material	218:248	a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses	218:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	4	38	theme	cellulose	741:749	arg1	microfibrils					751:762	cellulose microfibrils	741:762	cellulose microfibrils into ribbons or bundles	741:786	The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles.					
29628264	2	39	theme	enzymatic	369:377	arg1	hydrolysis					379:388	the enzymatic hydrolysis	365:388	the enzymatic hydrolysis with a commercial cellulase mixture	365:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	6	40	theme	genetic	1132:1138	arg1	engineering					1140:1150	genetic engineering	1132:1150	genetic engineering	1132:1150	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	2	41	theme	biomimetic	220:229	arg1	material					241:248	a biomimetic composite material	218:248	a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses	218:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	5	42	theme	bacterial	889:897	arg1	cellulose					899:907	bacterial cellulose	889:907	bacterial cellulose	889:907	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	3	43	theme	hemicellulose-free	574:591	arg1	cellulose					603:611	hemicellulose-free bacterial cellulose	574:611	hemicellulose-free bacterial cellulose	574:611	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	2	44	theme	wood-based	288:297	arg1	hemicelluloses					299:312	bacterial cellulose and wood-based hemicelluloses	264:312	bacterial cellulose and wood-based hemicelluloses	264:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	4	45	theme	microfibrils	751:762	arg1	packing					730:736	the packing	726:736	the packing of cellulose microfibrils into ribbons or bundles	726:786	The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles.					
29628264	5	46	theme	enzymatic	939:947	arg1	hydrolysis					949:958	the enzymatic hydrolysis	935:958	the enzymatic hydrolysis	935:958	In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis.					
29628264	6	47	theme	cellulose	1015:1023	arg1	hydrolysis					991:1000	The reported easier enzymatic hydrolysis	961:1000	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan	961:1068	The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.					
29628264	3	48	theme	Bacterial	427:435	arg1	cellulose					437:445	Bacterial cellulose	427:445	Bacterial cellulose synthesized in the presence of hemicelluloses	427:491	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	3	48	theme	Bacterial	427:435	arg1	xylan					505:509	xylan	505:509	especially xylan	494:509	Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose.					
29628264	2	49	theme	cellulase	408:416	arg1	mixture					418:424	a commercial cellulase mixture	395:424	a commercial cellulase mixture	395:424	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	2	50	used	used	318:321	arg2	material					241:248	a biomimetic composite material	218:248	a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses	218:312	Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture.					
29628264	4	51	theme	nanoscale	675:683	arg1	structure					685:693	the nanoscale structure	671:693	the nanoscale structure of the substrate	671:710	The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles.					
30530654	7	0	with	coformulation	1353:1365	arg1	glycoconjugates					1401:1415	both isolated polysaccharide glycoconjugates	1372:1415	both isolated polysaccharide glycoconjugates	1372:1415	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	6	1	theme	pentavalent	1125:1135	arg1	vaccine					1166:1172	A pentavalent semisynthetic glycoconjugate vaccine	1123:1172	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5)	1123:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	4	2	theme	marketed	750:757	arg1	vaccines					784:791	marketed CPS-based glycoconjugate vaccines	750:791	marketed CPS-based glycoconjugate vaccines	750:791	Here, we show that marketed CPS-based glycoconjugate vaccines can be improved by adding synthetic glycoconjugates representing serotypes that are not covered by existing vaccines.					
30530654	7	3	theme	multivalent	1505:1515	arg1	vaccines					1528:1535	multivalent conjugated vaccines	1505:1535	multivalent conjugated vaccines	1505:1535	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	6	4	contain	containing	1174:1183	arg1	vaccine					1166:1172	A pentavalent semisynthetic glycoconjugate vaccine	1123:1172	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5)	1123:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	6	4	contain	containing	1174:1183	arg2	sPCV5					1209:1213	sPCV5	1209:1213	sPCV5	1209:1213	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	6	4	contain	containing	1174:1183	arg2	antigens					1199:1206	five serotype antigens	1185:1206	five serotype antigens (sPCV5)	1185:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	1	5	theme	conjugate	173:181	arg1	vaccines					202:209	conjugate and polysaccharide vaccines	173:209	vaccines	202:209	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	7	6	theme	synthetic	1311:1319	arg1	oligosaccharides					1321:1336	synthetic oligosaccharides	1311:1336	synthetic oligosaccharides	1311:1336	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	5	7	theme	conjugate	1077:1085	arg1	vaccines					1087:1094	13-valent conjugate vaccines	1067:1094	13-valent conjugate vaccines	1067:1094	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	7	8	theme	conjugated	1517:1526	arg1	vaccines					1528:1535	multivalent conjugated vaccines	1505:1535	multivalent conjugated vaccines	1505:1535	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	6	9	theme	strong	1240:1245	arg1	activity					1273:1280	strong in vitro opsonophagocytic activity	1240:1280	strong in vitro opsonophagocytic activity	1240:1280	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	5	10	dep	vaccines	986:993	arg1	10-valent					1032:1040	10-valent	1032:1040	10-valent	1032:1040	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	10	dep	vaccines	986:993	arg1	13-valent					1006:1014	13-valent	1006:1014	13-valent	1006:1014	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	10	dep	vaccines	986:993	arg1	Synflorix					1021:1029	Synflorix	1021:1029	Synflorix (10-valent)	1021:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	10	dep	vaccines	986:993	arg1	Prevnar13					995:1003	Prevnar13	995:1003	Prevnar13 (13-valent)	995:1015	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	10	dep	vaccines	986:993	arg1	vaccines					986:993	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	4	11	theme	synthetic	819:827	arg1	glycoconjugates					829:843	synthetic glycoconjugates	819:843	synthetic glycoconjugates representing serotypes that are not covered by existing vaccines	819:908	Here, we show that marketed CPS-based glycoconjugate vaccines can be improved by adding synthetic glycoconjugates representing serotypes that are not covered by existing vaccines.					
30530654	1	12	theme	Streptococcus	77:89	arg1	pneumoniae					91:100	Streptococcus pneumoniae	77:100	Streptococcus pneumoniae	77:100	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	3	13	dep	stability	571:579	arg1	the					567:569	the	567:569	the	567:569	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	5	14	theme	synthetic	942:950	arg1	glycoconjugates					952:966	synthetic glycoconjugates	942:966	synthetic glycoconjugates	942:966	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	2	15	theme	serotype	408:415	arg1	distribution					417:428	geographic and demographic serotype distribution	381:428	geographic and demographic serotype distribution	381:428	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	7	16	dep	produce	1479:1485	arg1	defined					1497:1503	defined	1497:1503	to produce precisely defined multivalent conjugated vaccines	1476:1535	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	2	17	theme	common	285:290	arg1	serotypes					292:300	The most common serotypes	276:300	The most common serotypes that cause infection	276:321	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	6	18	theme	serotype	1190:1197	arg1	sPCV5					1209:1213	sPCV5	1209:1213	sPCV5	1209:1213	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	6	18	theme	serotype	1190:1197	arg1	antigens					1199:1206	five serotype antigens	1185:1206	five serotype antigens (sPCV5)	1185:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	2	19	theme	demographic	396:406	arg1	distribution					417:428	geographic and demographic serotype distribution	381:428	geographic and demographic serotype distribution	381:428	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	6	20	theme	opsonophagocytic	1256:1271	arg1	activity					1273:1280	strong in vitro opsonophagocytic activity	1240:1280	strong in vitro opsonophagocytic activity	1240:1280	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	3	21	theme	candidates	621:630	arg1	immunogenicity					585:598	immunogenicity	585:598	immunogenicity	585:598	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	21	theme	candidates	621:630	arg1	stability					571:579	stability	571:579	stability	571:579	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	5	22	theme	glycoconjugates	952:966	arg1	coformulation					924:936	coformulation	924:936	coformulation	924:936	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	22	theme	glycoconjugates	952:966	arg1	Combination					911:921	Combination	911:921	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	911:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	1	23	theme	deadly	112:117	arg1	disease					119:125	a deadly disease	110:125	a deadly disease in small children and the elderly	110:159	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	2	24	theme	geographic	381:390	arg1	distribution					417:428	geographic and demographic serotype distribution	381:428	geographic and demographic serotype distribution	381:428	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	2	25	from	differences	366:376	arg1	distribution					417:428	geographic and demographic serotype distribution	381:428	geographic and demographic serotype distribution	381:428	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	3	26	theme	synthetic	603:611	arg1	candidates					621:630	synthetic vaccine candidates	603:630	synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies	603:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	6	27	theme	semisynthetic	1137:1149	arg1	vaccine					1166:1172	A pentavalent semisynthetic glycoconjugate vaccine	1123:1172	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5)	1123:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	2	28	theme	leading	456:462	arg1	vaccines					464:471	leading vaccines	456:471	leading vaccines	456:471	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	3	29	theme	specific	699:706	arg1	antibodies					719:728	specific protective antibodies	699:728	specific protective antibodies	699:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	30	theme	vaccine	613:619	arg1	candidates					621:630	synthetic vaccine candidates	603:630	synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies	603:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	1	31	theme	isolated	220:227	arg1	CPS					255:257	CPS	255:257	CPS	255:257	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	1	31	theme	isolated	220:227	arg1	polysaccharides					238:252	isolated capsular polysaccharides	220:252	isolated capsular polysaccharides (CPS)	220:258	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	5	32	with	Combination	911:921	arg1	vaccines					986:993	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	32	with	Combination	911:921	arg1	Synflorix					1021:1029	Synflorix	1021:1029	Synflorix (10-valent)	1021:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	32	with	Combination	911:921	arg1	Prevnar13					995:1003	Prevnar13	995:1003	Prevnar13 (13-valent)	995:1015	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	3	33	theme	protective	708:717	arg1	antibodies					719:728	specific protective antibodies	699:728	specific protective antibodies	699:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	4	34	theme	existing	892:899	arg1	vaccines					901:908	existing vaccines	892:908	existing vaccines	892:908	Here, we show that marketed CPS-based glycoconjugate vaccines can be improved by adding synthetic glycoconjugates representing serotypes that are not covered by existing vaccines.					
30530654	6	35	theme	glycoconjugate	1151:1164	arg1	vaccine					1166:1172	A pentavalent semisynthetic glycoconjugate vaccine	1123:1172	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5)	1123:1214	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	1	36	theme	capsular	229:236	arg1	CPS					255:257	CPS	255:257	CPS	255:257	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	1	36	theme	capsular	229:236	arg1	polysaccharides					238:252	isolated capsular polysaccharides	220:252	isolated capsular polysaccharides (CPS)	220:258	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	6	37	dep	in	1247:1248	arg1	vitro					1250:1254	vitro	1250:1254	vitro	1250:1254	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	3	38	theme	levels	689:694	arg1	induction					669:677	the induction	665:677	the induction of higher levels of specific protective antibodies	665:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	39	theme	chemistry	488:496	arg1	approach					498:505	The medicinal chemistry approach	474:505	The medicinal chemistry approach to glycoconjugate vaccine development	474:543	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	40	theme	several	636:642	arg1	serotypes					644:652	several serotypes	636:652	several serotypes leading to the induction of higher levels of specific protective antibodies	636:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	6	41	with	antibodies	1224:1233	arg1	activity					1273:1280	strong in vitro opsonophagocytic activity	1240:1280	strong in vitro opsonophagocytic activity	1240:1280	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	1	42	theme	small	130:134	arg1	children					136:143	small children	130:143	small children	130:143	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	0	43	theme	synthetic	58:66	arg1	glycans					68:74	synthetic glycans	58:74	synthetic glycans	58:74	Improving vaccines against Streptococcus pneumoniae using synthetic glycans.					
30530654	4	44	theme	CPS-based	759:767	arg1	vaccines					784:791	marketed CPS-based glycoconjugate vaccines	750:791	marketed CPS-based glycoconjugate vaccines	750:791	Here, we show that marketed CPS-based glycoconjugate vaccines can be improved by adding synthetic glycoconjugates representing serotypes that are not covered by existing vaccines.					
30530654	1	45	from	disease	119:125	arg1	children					136:143	small children	130:143	small children	130:143	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	1	45	from	disease	119:125	arg1	elderly					153:159	elderly	153:159	elderly	153:159	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	7	46	theme	isolated	1377:1384	arg1	glycoconjugates					1401:1415	both isolated polysaccharide glycoconjugates	1372:1415	both isolated polysaccharide glycoconjugates	1372:1415	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	4	47	theme	glycoconjugate	769:782	arg1	vaccines					784:791	marketed CPS-based glycoconjugate vaccines	750:791	marketed CPS-based glycoconjugate vaccines	750:791	Here, we show that marketed CPS-based glycoconjugate vaccines can be improved by adding synthetic glycoconjugates representing serotypes that are not covered by existing vaccines.					
30530654	5	48	theme	13-valent	1067:1075	arg1	vaccines					1087:1094	13-valent conjugate vaccines	1067:1094	13-valent conjugate vaccines	1067:1094	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	7	49	used	used	1345:1348	arg2	oligosaccharides					1321:1336	synthetic oligosaccharides	1311:1336	synthetic oligosaccharides	1311:1336	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	3	50	theme	glycoconjugate	510:523	arg1	development					533:543	glycoconjugate vaccine development	510:543	glycoconjugate vaccine development	510:543	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	6	51	theme	in	1247:1248	arg1	activity					1273:1280	strong in vitro opsonophagocytic activity	1240:1280	strong in vitro opsonophagocytic activity	1240:1280	A pentavalent semisynthetic glycoconjugate vaccine containing five serotype antigens (sPCV5) elicits antibodies with strong in vitro opsonophagocytic activity.					
30530654	7	52	from	vaccines	1452:1459	arg1	protection					1427:1436	protection	1427:1436	protection from existing vaccines	1427:1459	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	3	53	theme	antibodies	719:728	arg1	levels					689:694	higher levels	682:694	higher levels of specific protective antibodies	682:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	54	theme	higher	682:687	arg1	levels					689:694	higher levels	682:694	higher levels of specific protective antibodies	682:728	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	3	55	theme	vaccine	525:531	arg1	development					533:543	glycoconjugate vaccine development	510:543	glycoconjugate vaccine development	510:543	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	2	56	used	used	327:330	arg2	serotypes					292:300	The most common serotypes	276:300	The most common serotypes that cause infection	276:321	The most common serotypes that cause infection are used in vaccines around the world, but differences in geographic and demographic serotype distribution compromises protection by leading vaccines.					
30530654	5	57	dep	yields	1043:1048	arg1	improved					1050:1057	improved	1050:1057	improved	1050:1057	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	3	58	theme	medicinal	478:486	arg1	approach					498:505	The medicinal chemistry approach	474:505	The medicinal chemistry approach to glycoconjugate vaccine development	474:543	The medicinal chemistry approach to glycoconjugate vaccine development has helped to improve the stability and immunogenicity of synthetic vaccine candidates for several serotypes leading to the induction of higher levels of specific protective antibodies.					
30530654	7	59	theme	existing	1443:1450	arg1	vaccines					1452:1459	existing vaccines	1443:1459	existing vaccines	1443:1459	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	1	60	theme	polysaccharide	187:200	arg1	vaccines					202:209	conjugate and polysaccharide vaccines	173:209	vaccines	202:209	Streptococcus pneumoniae remains a deadly disease in small children and the elderly even though conjugate and polysaccharide vaccines based on isolated capsular polysaccharides (CPS) are successful.					
30530654	7	61	theme	polysaccharide	1386:1399	arg1	glycoconjugates					1401:1415	both isolated polysaccharide glycoconjugates	1372:1415	both isolated polysaccharide glycoconjugates	1372:1415	This study illustrates that synthetic oligosaccharides can be used in coformulation with both isolated polysaccharide glycoconjugates to expand protection from existing vaccines and each other to produce precisely defined multivalent conjugated vaccines.					
30530654	5	62	theme	licensed	977:984	arg1	vaccines					986:993	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent)	973:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	62	theme	licensed	977:984	arg1	Synflorix					1021:1029	Synflorix	1021:1029	Synflorix (10-valent)	1021:1041	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30530654	5	62	theme	licensed	977:984	arg1	Prevnar13					995:1003	Prevnar13	995:1003	Prevnar13 (13-valent)	995:1015	Combination (coformulation) of synthetic glycoconjugates with the licensed vaccines Prevnar13 (13-valent) and Synflorix (10-valent) yields improved 15- and 13-valent conjugate vaccines, respectively, in rabbits.					
30503789	4	0	theme	atypical	723:730	arg1	details					748:754	atypical fine structural details	723:754	atypical fine structural details	723:754	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	4	1	theme	structural	737:746	arg1	details					748:754	atypical fine structural details	723:754	atypical fine structural details	723:754	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	4	2	theme	oligosaccharides	592:607	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	4	3	theme	Sugar	526:530	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	4	4	theme	UV-spectrometric	552:567	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	6	5	theme	region	887:892	arg1	chains					864:869	large neutral side chains	845:869	large neutral side chains of the ramified region and ester linked phenolic acids	845:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	2	6	theme	sequential	335:344	arg1	extraction					346:355	sequential extraction	335:355	sequential extraction with inorganic solvents	335:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	7	7	theme	homogeneous	1056:1066	arg1	complex					1082:1088	a homogeneous water-soluble complex	1054:1088	a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M)	1054:1151	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	0	8	with	activity	76:83	arg1	albumin					119:125	bovine serum albumin	106:125	bovine serum albumin	106:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	5	9	theme	dose-dependent	791:804	arg1	activity					818:825	dose-dependent antioxidant activity	791:825	dose-dependent antioxidant activity	791:825	Biochemical analysis demonstrated dose-dependent antioxidant activity.					
30503789	7	10	theme	binding	1117:1123	arg1	K = 2.91 × 106/M					1135:1150	K = 2.91 × 106/M	1135:1150	K = 2.91 × 106/M	1135:1150	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	7	10	theme	binding	1117:1123	arg1	constant					1125:1132	binding constant	1117:1132	binding constant	1117:1132	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	2	11	theme	chemical	264:271	arg1	compositions					273:284	chemical compositions	264:284	chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents	264:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	0	12	theme	serum	113:117	arg1	albumin					119:125	bovine serum albumin	106:125	bovine serum albumin	106:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	1	13	used	used	147:150	arg2	vulgaris					135:142	Thymus vulgaris	128:142	Thymus vulgaris	128:142	Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent.					
30503789	7	14	dep	albumin	1108:1114	arg1	K = 2.91 × 106/M					1135:1150	K = 2.91 × 106/M	1135:1150	K = 2.91 × 106/M	1135:1150	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	7	14	dep	albumin	1108:1114	arg1	constant					1125:1132	binding constant	1117:1132	binding constant	1117:1132	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	1	15	theme	health	164:169	arg1	benefits					171:178	various health benefits	156:178	various health benefits	156:178	Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent.					
30503789	0	16	theme	bovine	106:111	arg1	albumin					119:125	bovine serum albumin	106:125	bovine serum albumin	106:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	6	17	theme	functional	946:955	arg1	sites					957:961	the functional sites	942:961	the functional sites	942:961	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	17	theme	functional	946:955	arg1	combination					830:840	A combination	828:840	A combination of large neutral side chains of the ramified region and ester linked phenolic acids	828:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	18	theme	chains	864:869	arg1	sites					957:961	the functional sites	942:961	the functional sites	942:961	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	18	theme	chains	864:869	arg1	combination					830:840	A combination	828:840	A combination of large neutral side chains of the ramified region and ester linked phenolic acids	828:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	7	19	with	complex	1082:1088	arg1	albumin					1108:1114	bovine serum albumin	1095:1114	bovine serum albumin (binding constant, K = 2.91 × 106/M)	1095:1151	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	3	20	theme	I	445:445	arg1	polysaccharide					452:465	a unique rhamnogalacturonan I type polysaccharide	417:465	a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	417:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	8	21	theme	dietetic	1295:1302	arg1	antioxidant					1304:1314	natural dietetic antioxidant	1287:1314	natural dietetic antioxidant	1287:1314	Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.					
30503789	0	22	from	leaf	37:40	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.	0:126	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	8	23	theme	water	1168:1172	arg1	extraction					1174:1183	water extraction	1168:1183	water extraction	1168:1183	Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.					
30503789	6	24	theme	side	859:862	arg1	chains					864:869	large neutral side chains	845:869	large neutral side chains of the ramified region and ester linked phenolic acids	845:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	3	25	theme	chemical	397:404	arg1	profile					406:412	chemical profile	397:412	chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	397:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	6	26	link	linked	904:909	arg1	acids					920:924	ester linked phenolic acids	898:924	ester linked phenolic acids	898:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	8	27	theme	natural	1287:1293	arg1	antioxidant					1304:1314	natural dietetic antioxidant	1287:1314	natural dietetic antioxidant	1287:1314	Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.					
30503789	6	28	theme	acids	920:924	arg1	chains					864:869	large neutral side chains	845:869	large neutral side chains of the ramified region and ester linked phenolic acids	845:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	29	theme	neutral	851:857	arg1	chains					864:869	large neutral side chains	845:869	large neutral side chains of the ramified region and ester linked phenolic acids	845:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	2	30	theme	polysaccharides	293:307	arg1	compositions					273:284	chemical compositions	264:284	chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents	264:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	3	31	theme	unique	419:424	arg1	polysaccharide					452:465	a unique rhamnogalacturonan I type polysaccharide	417:465	a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	417:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	0	32	theme	vulgaris	28:35	arg1	leaf					37:40	Thymus vulgaris leaf	21:40	Thymus vulgaris leaf	21:40	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	2	33	attach	isolated	309:316	arg1	leaf					327:330	its leaf	323:330	its leaf	323:330	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	2	33	attach	isolated	309:316	arg2	polysaccharides					293:307	the polysaccharides	289:307	the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents	289:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	0	34	with	interaction	89:99	arg1	albumin					119:125	bovine serum albumin	106:125	bovine serum albumin	106:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	0	35	theme	Thymus	21:26	arg1	leaf					37:40	Thymus vulgaris leaf	21:40	Thymus vulgaris leaf	21:40	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	6	36	theme	large	845:849	arg1	chains					864:869	large neutral side chains	845:869	large neutral side chains of the ramified region and ester linked phenolic acids	845:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	37	theme	phenolic	911:918	arg1	acids					920:924	ester linked phenolic acids	898:924	ester linked phenolic acids	898:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	1	38	theme	various	156:162	arg1	benefits					171:178	various health benefits	156:178	various health benefits	156:178	Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent.					
30503789	3	39	theme	ester	478:482	arg1	acids					500:504	ester linked phenolic acids	478:504	ester linked phenolic acids	478:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	4	40	theme	ESI-MS	573:578	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	5	41	theme	Biochemical	757:767	arg1	analysis					769:776	Biochemical analysis	757:776	Biochemical analysis	757:776	Biochemical analysis demonstrated dose-dependent antioxidant activity.					
30503789	4	42	theme	acid	676:679	arg1	hydrolysis					681:690	controlled acid hydrolysis	665:690	controlled acid hydrolysis	665:690	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	4	43	theme	Smith	696:700	arg1	degradation					702:712	Smith degradation	696:712	Smith degradation	696:712	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	3	44	theme	type	447:450	arg1	polysaccharide					452:465	a unique rhamnogalacturonan I type polysaccharide	417:465	a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	417:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	3	45	theme	rhamnogalacturonan	426:443	arg1	polysaccharide					452:465	a unique rhamnogalacturonan I type polysaccharide	417:465	a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	417:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	3	46	theme	polysaccharide	452:465	arg1	profile					406:412	chemical profile	397:412	chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	397:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	4	47	theme	compositional	532:544	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	3	48	link	linked	484:489	arg1	acids					500:504	ester linked phenolic acids	478:504	ester linked phenolic acids	478:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	3	49	contain	containing	467:476	arg1	polysaccharide					452:465	a unique rhamnogalacturonan I type polysaccharide	417:465	a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids	417:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	3	49	contain	containing	467:476	arg2	acids					500:504	ester linked phenolic acids	478:504	ester linked phenolic acids	478:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	1	50	from	report	208:213	arg1	macromolecules					222:235	its macromolecules	218:235	its macromolecules	218:235	Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent.					
30503789	0	51	theme	Structural	43:52	arg1	features					54:61	Structural features	43:61	Structural features	43:61	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	2	52	with	extraction	346:355	arg1	solvents					372:379	inorganic solvents	362:379	inorganic solvents	362:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	7	53	theme	serum	1102:1106	arg1	albumin					1108:1114	bovine serum albumin	1095:1114	bovine serum albumin (binding constant, K = 2.91 × 106/M)	1095:1151	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	4	54	theme	TLC	547:549	arg1	analyses					580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses	526:587	analyses	580:587	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	6	55	theme	linked	904:909	arg1	acids					920:924	ester linked phenolic acids	898:924	ester linked phenolic acids	898:924	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	6	56	theme	ramified	878:885	arg1	region					887:892	the ramified region	874:892	the ramified region	874:892	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	1	57	theme	Thymus	128:133	arg1	vulgaris					135:142	Thymus vulgaris	128:142	Thymus vulgaris	128:142	Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent.					
30503789	4	58	theme	fine	732:735	arg1	details					748:754	atypical fine structural details	723:754	atypical fine structural details	723:754	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	5	59	theme	antioxidant	806:816	arg1	activity					818:825	dose-dependent antioxidant activity	791:825	dose-dependent antioxidant activity	791:825	Biochemical analysis demonstrated dose-dependent antioxidant activity.					
30503789	8	60	theme	pharmacological	1224:1238	arg1	effect					1240:1245	pharmacological effect	1224:1245	pharmacological effect	1224:1245	Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.					
30503789	8	61	theme	thyme	1278:1282	arg1	impact					1268:1273	the impact	1264:1273	the impact of thyme	1264:1282	Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.					
30503789	0	62	with	features	54:61	arg1	albumin					119:125	bovine serum albumin	106:125	bovine serum albumin	106:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	3	63	theme	phenolic	491:498	arg1	acids					500:504	ester linked phenolic acids	478:504	ester linked phenolic acids	478:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	7	64	theme	Ultraviolet	964:974	arg1	spectrometric					976:988	Ultraviolet spectrometric and fluorimetric analyses	964:1014	spectrometric	976:988	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	3	65	theme	linked	484:489	arg1	acids					500:504	ester linked phenolic acids	478:504	ester linked phenolic acids	478:504	In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described.					
30503789	0	66	theme	antioxidant	64:74	arg1	activity					76:83	antioxidant activity	64:83	antioxidant activity	64:83	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	4	67	theme	controlled	665:674	arg1	hydrolysis					681:690	controlled acid hydrolysis	665:690	controlled acid hydrolysis	665:690	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	7	68	theme	bovine	1095:1100	arg1	albumin					1108:1114	bovine serum albumin	1095:1114	bovine serum albumin (binding constant, K = 2.91 × 106/M)	1095:1151	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	2	69	theme	inorganic	362:370	arg1	solvents					372:379	inorganic solvents	362:379	inorganic solvents	362:379	Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents.					
30503789	7	70	theme	fluorimetric	994:1005	arg1	analyses					1007:1014	Ultraviolet spectrometric and fluorimetric analyses	964:1014	analyses	1007:1014	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30503789	4	71	theme	enzyme	647:652	arg1	digestion					654:662	enzyme digestion	647:662	enzyme digestion	647:662	Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details.					
30503789	6	72	dep	linked	904:909	arg1	ester					898:902	ester	898:902	ester	898:902	A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites.					
30503789	0	73	dep	Polysaccharides	0:14	arg1	activity					76:83	antioxidant activity	64:83	antioxidant activity	64:83	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	0	73	dep	Polysaccharides	0:14	arg1	interaction					89:99	interaction	89:99	interaction with bovine serum albumin	89:125	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	0	73	dep	Polysaccharides	0:14	arg1	features					54:61	Structural features	43:61	Structural features	43:61	Polysaccharides from Thymus vulgaris leaf: Structural features, antioxidant activity and interaction with bovine serum albumin.					
30503789	7	74	theme	water-soluble	1068:1080	arg1	complex					1082:1088	a homogeneous water-soluble complex	1054:1088	a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M)	1054:1151	Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 106/M).					
30693728	4	0	theme	powder	661:666	arg1	slices					668:673	powder slices	661:673	powder slices	661:673	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	7	1	theme	boiled	1409:1414	arg1	velvet					1423:1428	the boiled antler velvet	1405:1428	the boiled antler velvet	1405:1428	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	4	2	theme	boiled	712:717	arg1	velvet					726:731	the boiled antler velvet	708:731	the boiled antler velvet	708:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	2	3	theme	phenol-sulfuric	289:303	arg1	colorimetry					310:320	a phenol-sulfuric acid colorimetry	287:320	a phenol-sulfuric acid colorimetry	287:320	The content was determined by a phenol-sulfuric acid colorimetry.					
30693728	7	4	from	contents	1393:1400	arg1	velvet					1423:1428	the boiled antler velvet	1405:1428	the boiled antler velvet	1405:1428	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	4	5	theme	velvet	726:731	arg1	slices					698:703	bone slices	693:703	bone slices	693:703	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	5	theme	velvet	726:731	arg1	slices					653:658	wax slices	649:658	wax slices	649:658	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	5	theme	velvet	726:731	arg1	slices					668:673	powder slices	661:673	powder slices	661:673	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	5	theme	velvet	726:731	arg1	slices					682:687	gauze slices	676:687	gauze slices	676:687	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	6	theme	bone	693:696	arg1	slices					698:703	bone slices	693:703	bone slices	693:703	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	8	7	theme	bone	1826:1829	arg1	pieces					1831:1836	bone pieces	1826:1836	bone pieces	1826:1836	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	3	8	from	compositions	387:398	arg1	parts					448:452	different parts	438:452	different parts of the antler velvet after different processing methods	438:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	9	9	theme	theoretical	1861:1871	arg1	reference					1873:1881	a theoretical reference	1859:1881	a theoretical reference for velvet processing and product development	1859:1927	This study provides a theoretical reference for velvet processing and product development.					
30693728	1	10	theme	alcohol	234:240	arg1	precipitation					242:254	alcohol precipitation	234:254	alcohol precipitation	234:254	The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation.					
30693728	6	11	theme	glucuronic	1153:1162	arg1	monosaccharides					1106:1120	Eight monosaccharides	1100:1120	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose)	1100:1226	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	11	theme	glucuronic	1153:1162	arg1	acid					1164:1167	glucuronic acid	1153:1167	glucuronic acid	1153:1167	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	4	12	theme	1.13	759:762	arg1	g/kg					764:767	1.74, 1.67, 1.03 and 1.13 g/kg	738:767	g/kg	764:767	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	12	theme	1.13	759:762	arg1	contents					637:644	the water-soluble polysaccharide contents	604:644	the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet	604:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	8	13	theme	same	1717:1720	arg1	method					1722:1727	the same method	1713:1727	the same method	1713:1727	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	4	14	dep	g/kg	764:767	arg1	whereas					784:790	whereas	784:790	whereas	784:790	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	6	15	located	detected	1233:1240	arg2	acid					1183:1186	galacturonic acid	1170:1186	galacturonic acid	1170:1186	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	galactose					1217:1225	galactose	1217:1225	galactose	1217:1225	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	monosaccharides					1106:1120	Eight monosaccharides	1100:1120	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose)	1100:1226	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	ribose					1145:1150	ribose	1145:1150	ribose	1145:1150	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	mannose					1123:1129	mannose	1123:1129	mannose	1123:1129	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg1	compositions					1249:1260	the compositions	1245:1260	the compositions of water-soluble polysaccharides in the antler velvet	1245:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	aminogalactose					1189:1202	aminogalactose	1189:1202	aminogalactose	1189:1202	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	glucosamine					1132:1142	glucosamine	1132:1142	glucosamine	1132:1142	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	glucose					1205:1211	glucose	1205:1211	glucose	1205:1211	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	15	located	detected	1233:1240	arg2	acid					1164:1167	glucuronic acid	1153:1167	glucuronic acid	1153:1167	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	3	16	theme	various	364:370	arg1	compositions					387:398	the various monosaccharide compositions	360:398	the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods	360:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	17	from	compositions	101:112	arg1	Pantotrichum					129:140	Cervi Cornu Pantotrichum	117:140	Cervi Cornu Pantotrichum	117:140	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	9	18	theme	product	1909:1915	arg1	development					1917:1927	product development	1909:1927	product development	1909:1927	This study provides a theoretical reference for velvet processing and product development.					
30693728	4	19	theme	1.74	738:741	arg1	g/kg					764:767	1.74, 1.67, 1.03 and 1.13 g/kg	738:767	g/kg	764:767	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	19	theme	1.74	738:741	arg1	contents					637:644	the water-soluble polysaccharide contents	604:644	the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet	604:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	6	20	theme	processing	1335:1344	arg1	methods					1346:1352	different processing methods	1325:1352	different processing methods	1325:1352	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	5	21	theme	polysaccharide	901:914	arg1	contents					916:923	The water-soluble polysaccharide contents	883:923	The water-soluble polysaccharide contents in in the same four parts of the antler velvet	883:970	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	5	22	from	contents	916:923	arg1	parts					945:949	the same four parts	931:949	the same four parts	931:949	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	1	23	theme	antler	181:186	arg1	velvet					188:193	antler velvet	181:193	antler velvet	181:193	The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation.					
30693728	3	24	theme	water-soluble	403:415	arg1	polysaccharides					417:431	water-soluble polysaccharides	403:431	water-soluble polysaccharides from different parts of the antler velvet after different processing methods	403:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	25	from	[Effects	0:7	arg1	contents					73:80	water soluble polysaccharide contents	44:80	water soluble polysaccharide contents	44:80	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	0	25	from	[Effects	0:7	arg1	compositions					101:112	monosaccharide compositions	86:112	monosaccharide compositions	86:112	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	0	26	theme	Cervi	117:121	arg1	Pantotrichum					129:140	Cervi Cornu Pantotrichum	117:140	Cervi Cornu Pantotrichum	117:140	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	3	27	from	parts	448:452	arg1	compositions					387:398	the various monosaccharide compositions	360:398	the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods	360:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	27	from	parts	448:452	arg1	polysaccharides					417:431	water-soluble polysaccharides	403:431	water-soluble polysaccharides from different parts of the antler velvet after different processing methods	403:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	28	theme	different	481:489	arg1	methods					502:508	different processing methods	481:508	different processing methods	481:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	4	29	from	contents	637:644	arg1	slices					698:703	bone slices	693:703	bone slices	693:703	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	29	from	contents	637:644	arg1	slices					653:658	wax slices	649:658	wax slices	649:658	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	29	from	contents	637:644	arg1	slices					668:673	powder slices	661:673	powder slices	661:673	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	29	from	contents	637:644	arg1	slices					682:687	gauze slices	676:687	gauze slices	676:687	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	30	theme	water-soluble	608:620	arg1	g/kg					764:767	1.74, 1.67, 1.03 and 1.13 g/kg	738:767	g/kg	764:767	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	30	theme	water-soluble	608:620	arg1	contents					637:644	the water-soluble polysaccharide contents	604:644	the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet	604:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	3	31	theme	ultra	527:531	arg1	chromatography					557:570	ultra high performance liquid chromatography	527:570	ultra high performance liquid chromatography (UPLC)	527:577	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	31	theme	ultra	527:531	arg1	UPLC					573:576	UPLC	573:576	UPLC	573:576	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	32	theme	different	12:20	arg1	methods					33:39	different processing methods	12:39	different processing methods	12:39	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	5	33	theme	antler	958:963	arg1	velvet					965:970	the antler velvet	954:970	the antler velvet	954:970	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	1	34	theme	water-soluble	148:160	arg1	polysaccharides					162:176	The water-soluble polysaccharides	144:176	The water-soluble polysaccharides in antler velvet	144:193	The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation.					
30693728	3	35	theme	velvet	468:473	arg1	parts					448:452	different parts	438:452	different parts of the antler velvet after different processing methods	438:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	36	theme	methods	33:39	arg1	[Effects					0:7	[Effects	0:7	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum	0:140	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	7	37	from	contents	1510:1517	arg1	velvet					1582:1587	the antler velvet	1571:1587	the antler velvet processed without blood	1571:1611	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	7	38	theme	freeze-dried	1459:1470	arg1	velvet					1479:1484	the freeze-dried antler velvet	1455:1484	the freeze-dried antler velvet	1455:1484	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	0	39	theme	polysaccharide	58:71	arg1	contents					73:80	water soluble polysaccharide contents	44:80	water soluble polysaccharide contents	44:80	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	0	40	theme	water	44:48	arg1	contents					73:80	water soluble polysaccharide contents	44:80	water soluble polysaccharide contents	44:80	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	6	41	from	compositions	1249:1260	arg1	velvet					1309:1314	the antler velvet	1298:1314	the antler velvet	1298:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	4	42	theme	antler	818:823	arg1	velvet					825:830	the freeze-dried antler velvet	801:830	the freeze-dried antler velvet	801:830	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	3	43	theme	performance	538:548	arg1	chromatography					557:570	ultra high performance liquid chromatography	527:570	ultra high performance liquid chromatography (UPLC)	527:577	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	43	theme	performance	538:548	arg1	UPLC					573:576	UPLC	573:576	UPLC	573:576	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	4	44	theme	gauze	676:680	arg1	slices					682:687	gauze slices	676:687	gauze slices	676:687	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	6	45	theme	different	1325:1333	arg1	methods					1346:1352	different processing methods	1325:1352	different processing methods	1325:1352	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	7	46	theme	antler	1416:1421	arg1	velvet					1423:1428	the boiled antler velvet	1405:1428	the boiled antler velvet	1405:1428	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	4	47	theme	wax	649:651	arg1	slices					653:658	wax slices	649:658	wax slices	649:658	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	7	48	theme	monosaccharide	1378:1391	arg1	contents					1393:1400	the monosaccharide contents	1374:1400	the monosaccharide contents in the boiled antler velvet	1374:1428	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	7	48	theme	monosaccharide	1378:1391	arg1	lower					1435:1439	lower	1435:1439	lower	1435:1439	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	8	49	theme	gauze	1809:1813	arg1	slices					1815:1820	gauze slices	1809:1820	gauze slices	1809:1820	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	2	50	theme	acid	305:308	arg1	colorimetry					310:320	a phenol-sulfuric acid colorimetry	287:320	a phenol-sulfuric acid colorimetry	287:320	The content was determined by a phenol-sulfuric acid colorimetry.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	aminogalactose					1189:1202	aminogalactose	1189:1202	aminogalactose	1189:1202	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	mannose					1123:1129	mannose	1123:1129	mannose	1123:1129	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	glucose					1205:1211	glucose	1205:1211	glucose	1205:1211	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	acid					1164:1167	glucuronic acid	1153:1167	glucuronic acid	1153:1167	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	glucosamine					1132:1142	glucosamine	1132:1142	glucosamine	1132:1142	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	acid					1183:1186	galacturonic acid	1170:1186	galacturonic acid	1170:1186	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	galactose					1217:1225	galactose	1217:1225	galactose	1217:1225	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	monosaccharides					1106:1120	Eight monosaccharides	1100:1120	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose)	1100:1226	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	51	dep	monosaccharides	1106:1120	arg1	ribose					1145:1150	ribose	1145:1150	ribose	1145:1150	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	7	52	theme	antler	1575:1580	arg1	velvet					1582:1587	the antler velvet	1571:1587	the antler velvet processed without blood	1571:1611	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	7	53	theme	same	1363:1366	arg1	part					1368:1371	the same part	1359:1371	the same part	1359:1371	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	4	54	theme	antler	719:724	arg1	velvet					726:731	the boiled antler velvet	708:731	the boiled antler velvet	708:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	8	55	theme	monosaccharide	1734:1747	arg1	contents					1749:1756	the monosaccharide contents	1730:1756	the monosaccharide contents in wax and powder pieces	1730:1781	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	8	55	theme	monosaccharide	1734:1747	arg1	higher					1788:1793	higher	1788:1793	higher	1788:1793	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	1	56	theme	water	213:217	arg1	extraction					219:228	water extraction	213:228	water extraction	213:228	The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation.					
30693728	6	57	theme	water-soluble	1265:1277	arg1	polysaccharides					1279:1293	water-soluble polysaccharides	1265:1293	water-soluble polysaccharides in the antler velvet	1265:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	0	58	from	contents	73:80	arg1	Pantotrichum					129:140	Cervi Cornu Pantotrichum	117:140	Cervi Cornu Pantotrichum	117:140	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	6	59	theme	galacturonic	1170:1181	arg1	monosaccharides					1106:1120	Eight monosaccharides	1100:1120	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose)	1100:1226	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	59	theme	galacturonic	1170:1181	arg1	acid					1183:1186	galacturonic acid	1170:1186	galacturonic acid	1170:1186	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	8	60	theme	different	1682:1690	arg1	parts					1692:1696	different parts	1682:1696	different parts processed with the same method	1682:1727	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	9	61	theme	velvet	1887:1892	arg1	processing					1894:1903	velvet processing	1887:1903	velvet processing	1887:1903	This study provides a theoretical reference for velvet processing and product development.					
30693728	8	62	from	contents	1749:1756	arg1	wax					1761:1763	wax	1761:1763	wax	1761:1763	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	8	62	from	contents	1749:1756	arg1	pieces					1776:1781	powder pieces	1769:1781	powder pieces	1769:1781	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	5	63	theme	same	935:938	arg1	parts					945:949	the same four parts	931:949	the same four parts	931:949	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	5	64	dep	processed	972:980	arg1	1.55					1024:1027	1.55	1024:1027	1.55	1024:1027	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	5	64	dep	processed	972:980	arg1	without					996:1002	without	996:1002	without	996:1002	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	5	65	theme	water-soluble	887:899	arg1	contents					916:923	The water-soluble polysaccharide contents	883:923	The water-soluble polysaccharide contents in in the same four parts of the antler velvet	883:970	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	3	66	theme	monosaccharide	372:385	arg1	compositions					387:398	the various monosaccharide compositions	360:398	the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods	360:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	67	theme	monosaccharide	86:99	arg1	compositions					101:112	monosaccharide compositions	86:112	monosaccharide compositions	86:112	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	7	68	theme	monosaccharide	1495:1508	arg1	contents					1510:1517	the monosaccharide contents	1491:1517	the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood	1491:1611	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	7	68	theme	monosaccharide	1495:1508	arg1	lower					1618:1622	lower	1618:1622	lower	1618:1622	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	6	69	theme	polysaccharides	1279:1293	arg1	compositions					1249:1260	the compositions	1245:1260	the compositions of water-soluble polysaccharides in the antler velvet	1245:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	5	70	theme	velvet	965:970	arg1	contents					916:923	The water-soluble polysaccharide contents	883:923	The water-soluble polysaccharide contents in in the same four parts of the antler velvet	883:970	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	7	71	theme	antler	1642:1647	arg1	velvet					1649:1654	the antler velvet	1638:1654	the antler velvet processed with blood	1638:1675	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	3	72	theme	polysaccharides	417:431	arg1	compositions					387:398	the various monosaccharide compositions	360:398	the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods	360:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	73	theme	Cornu	123:127	arg1	Pantotrichum					129:140	Cervi Cornu Pantotrichum	117:140	Cervi Cornu Pantotrichum	117:140	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	1	74	from	polysaccharides	162:176	arg1	velvet					188:193	antler velvet	181:193	antler velvet	181:193	The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation.					
30693728	5	75	theme	antler	982:987	arg1	velvet					989:994	antler velvet	982:994	antler velvet	982:994	The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively.					
30693728	6	76	theme	antler	1302:1307	arg1	velvet					1309:1314	the antler velvet	1298:1314	the antler velvet	1298:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	6	77	from	velvet	1309:1314	arg1	compositions					1249:1260	the compositions	1245:1260	the compositions of water-soluble polysaccharides in the antler velvet	1245:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	4	78	theme	polysaccharide	622:635	arg1	g/kg					764:767	1.74, 1.67, 1.03 and 1.13 g/kg	738:767	g/kg	764:767	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	4	78	theme	polysaccharide	622:635	arg1	contents					637:644	the water-soluble polysaccharide contents	604:644	the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet	604:731	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	3	79	theme	processing	491:500	arg1	methods					502:508	different processing methods	481:508	different processing methods	481:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	0	80	theme	processing	22:31	arg1	methods					33:39	different processing methods	12:39	different processing methods	12:39	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	8	81	theme	powder	1769:1774	arg1	pieces					1776:1781	powder pieces	1769:1781	powder pieces	1769:1781	For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces.					
30693728	3	82	theme	different	438:446	arg1	parts					448:452	different parts	438:452	different parts of the antler velvet after different processing methods	438:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	83	theme	high	533:536	arg1	chromatography					557:570	ultra high performance liquid chromatography	527:570	ultra high performance liquid chromatography (UPLC)	527:577	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	83	theme	high	533:536	arg1	UPLC					573:576	UPLC	573:576	UPLC	573:576	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	6	84	from	polysaccharides	1279:1293	arg1	velvet					1309:1314	the antler velvet	1298:1314	the antler velvet	1298:1314	Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods.					
30693728	0	85	theme	soluble	50:56	arg1	contents					73:80	water soluble polysaccharide contents	44:80	water soluble polysaccharide contents	44:80	[Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum].					
30693728	7	86	theme	antler	1472:1477	arg1	velvet					1479:1484	the freeze-dried antler velvet	1455:1484	the freeze-dried antler velvet	1455:1484	For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood.					
30693728	3	87	theme	antler	461:466	arg1	velvet					468:473	the antler velvet	457:473	the antler velvet after different processing methods	457:508	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	4	88	theme	freeze-dried	805:816	arg1	velvet					825:830	the freeze-dried antler velvet	801:830	the freeze-dried antler velvet	801:830	The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively.					
30693728	3	89	theme	liquid	550:555	arg1	chromatography					557:570	ultra high performance liquid chromatography	527:570	ultra high performance liquid chromatography (UPLC)	527:577	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
30693728	3	89	theme	liquid	550:555	arg1	UPLC					573:576	UPLC	573:576	UPLC	573:576	After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC).					
31301102	5	0	gly	glycosylation	962:974	arg1	antibodies					932:941	antibodies	932:941	antibodies with typical plant glycosylation	932:974	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	7	1	theme	plant	1321:1325	arg1	glycosylation					1327:1339	plant glycosylation	1321:1339	plant glycosylation	1321:1339	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	0	2	theme	Fc	77:78	arg1	receptors					80:88	human Fc receptors	71:88	human Fc receptors	71:88	Engineering the interactions between a plant-produced HIV antibody and human Fc receptors.					
31301102	5	3	contain	have	976:979	arg1	antibodies					932:941	antibodies	932:941	antibodies with typical plant glycosylation	932:974	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	5	3	contain	have	976:979	arg2	capacity					991:998	a limited capacity	981:998	a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa	981:1045	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	3	4	theme	different	509:517	arg1	glycovariants					519:531	different glycovariants	509:531	different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody,	509:589	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	6	5	contain	had	1180:1182	arg2	affinity					1203:1210	a slightly reduced affinity	1184:1210	a slightly reduced affinity to the neonatal Fc receptor (FcRn)	1184:1245	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	6	5	contain	had	1180:1182	arg1	glycovariants					1166:1178	All plant-made glycovariants	1151:1178	All plant-made glycovariants	1151:1178	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	6	6	theme	cell-derived	1265:1276	arg1	antibody					1278:1285	HEK cell-derived antibody	1261:1285	HEK cell-derived antibody	1261:1285	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	3	7	theme	broadly	545:551	arg1	VRC01					536:540	VRC01	536:540	VRC01	536:540	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	7	theme	broadly	545:551	arg1	antibody					581:588	a broadly neutralizing HIV monoclonal antibody	543:588	a broadly neutralizing HIV monoclonal antibody	543:588	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	7	8	theme	glycosylation	1327:1339	arg1	independent					1306:1316	independent	1306:1316	independent	1306:1316	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	2	9	theme	Fc	299:300	arg1	region					302:307	the antibody Fc region	286:307	the antibody Fc region	286:307	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	2	10	theme	Fc	341:342	arg1	receptors					344:352	Fc receptors	341:352	Fc receptors	341:352	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	5	11	theme	Surface	795:801	arg1	assays					827:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	6	12	theme	Fc	1228:1229	arg1	FcRn					1241:1244	FcRn	1241:1244	FcRn	1241:1244	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	6	12	theme	Fc	1228:1229	arg1	receptor					1231:1238	the neonatal Fc receptor	1215:1238	the neonatal Fc receptor (FcRn)	1215:1245	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	3	13	theme	human	489:493	arg1	receptors					498:506	human Fc receptors	489:506	human Fc receptors	489:506	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	2	14	theme	antibody	290:297	arg1	region					302:307	the antibody Fc region	286:307	the antibody Fc region	286:307	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	2	15	theme	antibody	399:406	arg1	functions					417:425	antibody effector functions	399:425	antibody effector functions	399:425	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	1	16	theme	therapeutic	169:179	arg1	antibodies					192:201	therapeutic monoclonal antibodies	169:201	therapeutic monoclonal antibodies	169:201	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	5	17	theme	plasmon	803:809	arg1	assays					827:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	1	18	theme	monoclonal	181:190	arg1	antibodies					192:201	therapeutic monoclonal antibodies	169:201	therapeutic monoclonal antibodies	169:201	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	5	19	theme	antibody-FcγR	886:898	arg1	interactions					900:911	antibody-FcγR interactions	886:911	antibody-FcγR interactions	886:911	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	5	20	theme	resonance-based	811:825	arg1	assays					827:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays	795:832	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	3	21	theme	monoclonal	570:579	arg1	VRC01					536:540	VRC01	536:540	VRC01	536:540	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	21	theme	monoclonal	570:579	arg1	antibody					581:588	a broadly neutralizing HIV monoclonal antibody	543:588	a broadly neutralizing HIV monoclonal antibody	543:588	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	1	22	theme	antibodies	192:201	arg1	production					155:164	production	155:164	production of therapeutic monoclonal antibodies	155:201	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	5	23	theme	interactions	900:911	arg1	evaluation					872:881	kinetic/affinity evaluation	855:881	kinetic/affinity evaluation of antibody-FcγR interactions	855:911	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	5	24	theme	typical	948:954	arg1	glycosylation					962:974	typical plant glycosylation	948:974	typical plant glycosylation	948:974	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	5	25	theme	plant	956:960	arg1	glycosylation					962:974	typical plant glycosylation	948:974	typical plant glycosylation	948:974	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	4	26	theme	characteristic	692:705	arg1	α1,3-fucose					707:717	plant characteristic α1,3-fucose and β1,2-xylose residues	686:742	α1,3-fucose	707:717	These include glycovariants lacking plant characteristic α1,3-fucose and β1,2-xylose residues and glycans extended with terminal β1,4-galactose.					
31301102	2	27	theme	Glycan	265:270	arg1	structures					272:281	Glycan structures	265:281	Glycan structures in the antibody Fc region	265:307	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	5	28	with	antibodies	932:941	arg1	glycosylation					962:974	typical plant glycosylation	948:974	typical plant glycosylation	948:974	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	3	29	theme	neutralizing	553:564	arg1	VRC01					536:540	VRC01	536:540	VRC01	536:540	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	29	theme	neutralizing	553:564	arg1	antibody					581:588	a broadly neutralizing HIV monoclonal antibody	543:588	a broadly neutralizing HIV monoclonal antibody	543:588	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	30	theme	VRC01	536:540	arg1	glycovariants					519:531	different glycovariants	509:531	different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody,	509:589	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	31	theme	HIV	566:568	arg1	VRC01					536:540	VRC01	536:540	VRC01	536:540	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	31	theme	HIV	566:568	arg1	antibody					581:588	a broadly neutralizing HIV monoclonal antibody	543:588	a broadly neutralizing HIV monoclonal antibody	543:588	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	7	32	theme	methionine	1385:1394	arg1	residues					1396:1403	two methionine residues	1381:1403	two methionine residues	1381:1403	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	0	33	theme	plant-produced	39:52	arg1	antibody					58:65	a plant-produced HIV antibody	37:65	a plant-produced HIV antibody	37:65	Engineering the interactions between a plant-produced HIV antibody and human Fc receptors.					
31301102	6	34	theme	neonatal	1219:1226	arg1	FcRn					1241:1244	FcRn	1241:1244	FcRn	1241:1244	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	6	34	theme	neonatal	1219:1226	arg1	receptor					1231:1238	the neonatal Fc receptor	1215:1238	the neonatal Fc receptor (FcRn)	1215:1245	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	2	35	from	structures	272:281	arg1	region					302:307	the antibody Fc region	286:307	the antibody Fc region	286:307	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	2	36	theme	functions	417:425	arg1	modulation					385:394	modulation	385:394	modulation of antibody effector functions	385:425	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	5	37	theme	kinetic/affinity	855:870	arg1	evaluation					872:881	kinetic/affinity evaluation	855:881	kinetic/affinity evaluation of antibody-FcγR interactions	855:911	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	8	38	theme	plant-produced	1530:1543	arg1	antibodies					1545:1554	plant-produced antibodies	1530:1554	plant-produced antibodies	1530:1554	This points towards a need for process optimization to control oxidation levels and improve the quality of plant-produced antibodies.					
31301102	8	39	theme	process	1454:1460	arg1	optimization					1462:1473	process optimization	1454:1473	process optimization	1454:1473	This points towards a need for process optimization to control oxidation levels and improve the quality of plant-produced antibodies.					
31301102	7	40	from	status	1371:1376	arg1	region					1415:1420	the Fc region	1408:1420	the Fc region	1408:1420	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	6	41	theme	plant-made	1155:1164	arg1	glycovariants					1166:1178	All plant-made glycovariants	1151:1178	All plant-made glycovariants	1151:1178	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	3	42	theme	glycosylation	450:462	arg1	impact					440:445	the impact	436:445	the impact of glycosylation in detail	436:472	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	3	43	theme	Fc	495:496	arg1	receptors					498:506	human Fc receptors	489:506	human Fc receptors	489:506	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	0	44	theme	HIV	54:56	arg1	antibody					58:65	a plant-produced HIV antibody	37:65	a plant-produced HIV antibody	37:65	Engineering the interactions between a plant-produced HIV antibody and human Fc receptors.					
31301102	6	45	theme	HEK	1261:1263	arg1	antibody					1278:1285	HEK cell-derived antibody	1261:1285	HEK cell-derived antibody	1261:1285	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	8	46	theme	antibodies	1545:1554	arg1	quality					1519:1525	the quality	1515:1525	the quality of plant-produced antibodies	1515:1554	This points towards a need for process optimization to control oxidation levels and improve the quality of plant-produced antibodies.					
31301102	7	47	theme	Fc	1412:1413	arg1	region					1415:1420	the Fc region	1408:1420	the Fc region	1408:1420	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	1	48	theme	cost-effective	112:125	arg1	technology					140:149	a cost-effective and scalable technology	110:149	a cost-effective and scalable technology for production of therapeutic monoclonal antibodies	110:201	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	5	49	theme	targeted	1124:1131	arg1	glycoengineering					1133:1148	targeted glycoengineering	1124:1148	targeted glycoengineering	1124:1148	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	5	50	theme	binding	1061:1067	arg1	characteristics					1069:1083	the binding characteristics	1057:1083	the binding characteristics	1057:1083	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	4	51	theme	terminal	770:777	arg1	β1,4-galactose					779:792	terminal β1,4-galactose	770:792	terminal β1,4-galactose	770:792	These include glycovariants lacking plant characteristic α1,3-fucose and β1,2-xylose residues and glycans extended with terminal β1,4-galactose.					
31301102	2	52	theme	binding	319:325	arg1	properties					327:336	binding properties	319:336	binding properties	319:336	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	1	53	theme	precise	227:233	arg1	engineering					235:245	precise engineering	227:245	precise engineering of glycosylation	227:262	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	8	54	theme	oxidation	1486:1494	arg1	levels					1496:1501	oxidation levels	1486:1501	oxidation levels	1486:1501	This points towards a need for process optimization to control oxidation levels and improve the quality of plant-produced antibodies.					
31301102	3	55	from	impact	440:445	arg1	detail					467:472	detail	467:472	detail	467:472	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	1	56	theme	scalable	131:138	arg1	technology					140:149	a cost-effective and scalable technology	110:149	a cost-effective and scalable technology for production of therapeutic monoclonal antibodies	110:201	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31301102	7	57	theme	residues	1396:1403	arg1	status					1371:1376	the oxidation status	1357:1376	the oxidation status of two methionine residues in the Fc region	1357:1420	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	0	58	theme	human	71:75	arg1	receptors					80:88	human Fc receptors	71:88	human Fc receptors	71:88	Engineering the interactions between a plant-produced HIV antibody and human Fc receptors.					
31301102	6	59	theme	reduced	1195:1201	arg1	affinity					1203:1210	a slightly reduced affinity	1184:1210	a slightly reduced affinity to the neonatal Fc receptor (FcRn)	1184:1245	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	3	60	theme	Nicotiana	609:617	arg1	benthamiana					619:629	Nicotiana benthamiana	609:629	Nicotiana benthamiana	609:629	To test the impact of glycosylation in detail, on binding to human Fc receptors, different glycovariants of VRC01, a broadly neutralizing HIV monoclonal antibody, were generated in Nicotiana benthamiana and characterized.					
31301102	4	61	theme	β1,2-xylose	723:733	arg1	residues					735:742	plant characteristic α1,3-fucose and β1,2-xylose residues	686:742	residues	735:742	These include glycovariants lacking plant characteristic α1,3-fucose and β1,2-xylose residues and glycans extended with terminal β1,4-galactose.					
31301102	2	62	theme	effector	408:415	arg1	functions					417:425	antibody effector functions	399:425	antibody effector functions	399:425	Glycan structures in the antibody Fc region influence binding properties to Fc receptors, which opens opportunities for modulation of antibody effector functions.					
31301102	6	63	link	cell-derived	1265:1276	arg1	antibody					1278:1285	HEK cell-derived antibody	1261:1285	HEK cell-derived antibody	1261:1285	All plant-made glycovariants had a slightly reduced affinity to the neonatal Fc receptor (FcRn) compared with HEK cell-derived antibody.					
31301102	7	64	theme	oxidation	1361:1369	arg1	status					1371:1376	the oxidation status	1357:1376	the oxidation status of two methionine residues in the Fc region	1357:1420	However, this was independent of plant glycosylation, but related to the oxidation status of two methionine residues in the Fc region.					
31301102	5	65	theme	limited	983:989	arg1	capacity					991:998	a limited capacity	981:998	a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa	981:1045	Surface plasmon resonance-based assays were established for kinetic/affinity evaluation of antibody-FcγR interactions, and revealed that antibodies with typical plant glycosylation have a limited capacity to engage FcγRI, FcγRIIa, FcγRIIb and FcγRIIIa; however, the binding characteristics can be restored and even improved with targeted glycoengineering.					
31301102	1	66	theme	glycosylation	250:262	arg1	engineering					235:245	precise engineering	227:245	precise engineering of glycosylation	227:262	Plants can provide a cost-effective and scalable technology for production of therapeutic monoclonal antibodies, with the potential for precise engineering of glycosylation.					
31022552	0	0	theme	myricetin	75:83	arg1	Development					0:10	Development	0:10	Development of M10	0:17	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	0	theme	myricetin	75:83	arg1	derivative					61:70	a derivative	59:70	a derivative of myricetin as a potent agent of anti-chronic colonic inflammation	59:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	7	1	theme	potential	1067:1075	arg1	agent					1089:1093	a potential therapeutic agent	1065:1093	a potential therapeutic agent for treatment of ulcerative colitis	1065:1129	Based on these results, M10 could be developed as a potential therapeutic agent for treatment of ulcerative colitis.					
31022552	7	1	theme	potential	1067:1075	arg1	M10					1039:1041	M10	1039:1041	M10	1039:1041	Based on these results, M10 could be developed as a potential therapeutic agent for treatment of ulcerative colitis.					
31022552	5	2	theme	sodium	889:894	arg1	model					915:919	a dextran sulfate sodium (DSS) induced mice model	871:919	a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis	871:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	1	3	theme	anti-inflammatory	239:255	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	6	4	from	safety	983:988	arg1	mice					1009:1012	mice	1009:1012	mice	1009:1012	In addition, M10 also exhibited high safety (LD50 > 5 g/kg) in mice.					
31022552	2	5	theme	poor	370:373	arg1	water-solubility					375:390	poor water-solubility	370:390	poor water-solubility	370:390	However, myricetin exhibited substantial limitations, such as poor water-solubility, and low stability in body when it was administrated by oral.					
31022552	4	6	theme	rat	760:762	arg1	plasma					764:769	Wistar rat plasma and liver microsomes	753:790	plasma	764:769	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	7	theme	salt	655:658	arg1	derivative					660:669	a sodium salt derivative	646:669	a sodium salt derivative	646:669	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	1	8	theme	anti-carcinogenic	258:274	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	4	9	theme	better	733:738	arg1	stability					740:748	better stability	733:748	better stability in Wistar rat plasma and liver microsomes	733:790	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	10	theme	Wistar	753:758	arg1	plasma					764:769	Wistar rat plasma and liver microsomes	753:790	plasma	764:769	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	11	theme	excellent	688:696	arg1	>100 mg/mL					716:725	>100 mg/mL	716:725	>100 mg/mL	716:725	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	11	theme	excellent	688:696	arg1	water-solubility					698:713	excellent water-solubility	688:713	excellent water-solubility (>100 mg/mL)	688:726	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	12	from	derivative	660:669	arg1	plasma					764:769	Wistar rat plasma and liver microsomes	753:790	plasma	764:769	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	12	from	derivative	660:669	arg1	microsomes					781:790	Wistar rat plasma and liver microsomes	753:790	microsomes	781:790	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	1	13	theme	natural	156:162	arg1	Myricetin					141:149	Myricetin	141:149	Myricetin	141:149	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	13	theme	natural	156:162	arg1	compound					182:189	a natural dietary flavonoid compound	154:189	a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	154:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	0	14	theme	potent	90:95	arg1	agent					97:101	a potent agent	88:101	a potent agent of anti-chronic colonic inflammation	88:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	6	15	theme	high	978:981	arg1	LD50 > 5 g/kg					991:1003	LD50 > 5 g/kg	991:1003	LD50 > 5 g/kg	991:1003	In addition, M10 also exhibited high safety (LD50 > 5 g/kg) in mice.					
31022552	6	15	theme	high	978:981	arg1	safety					983:988	high safety	978:988	high safety (LD50 > 5 g/kg) in mice	978:1012	In addition, M10 also exhibited high safety (LD50 > 5 g/kg) in mice.					
31022552	1	16	theme	dietary	164:170	arg1	Myricetin					141:149	Myricetin	141:149	Myricetin	141:149	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	16	theme	dietary	164:170	arg1	compound					182:189	a natural dietary flavonoid compound	154:189	a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	154:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	4	17	from	stability	740:748	arg1	plasma					764:769	Wistar rat plasma and liver microsomes	753:790	plasma	764:769	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	17	from	stability	740:748	arg1	microsomes					781:790	Wistar rat plasma and liver microsomes	753:790	microsomes	781:790	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	2	18	theme	substantial	337:347	arg1	water-solubility					375:390	poor water-solubility	370:390	poor water-solubility	370:390	However, myricetin exhibited substantial limitations, such as poor water-solubility, and low stability in body when it was administrated by oral.					
31022552	2	18	theme	substantial	337:347	arg1	limitations					349:359	substantial limitations	337:359	substantial limitations	337:359	However, myricetin exhibited substantial limitations, such as poor water-solubility, and low stability in body when it was administrated by oral.					
31022552	1	19	theme	flavonoid	172:180	arg1	Myricetin					141:149	Myricetin	141:149	Myricetin	141:149	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	19	theme	flavonoid	172:180	arg1	compound					182:189	a natural dietary flavonoid compound	154:189	a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	154:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	20	theme	anti-proliferative	280:297	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	2	21	theme	low	397:399	arg1	stability					401:409	low stability	397:409	low stability	397:409	However, myricetin exhibited substantial limitations, such as poor water-solubility, and low stability in body when it was administrated by oral.					
31022552	5	22	with	model	915:919	arg1	colitis					937:943	ulcerative colitis	926:943	ulcerative colitis	926:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	0	23	theme	anti-chronic	106:117	arg1	inflammation					127:138	anti-chronic colonic inflammation	106:138	anti-chronic colonic inflammation	106:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	5	24	theme	ulcerative	926:935	arg1	colitis					937:943	ulcerative colitis	926:943	ulcerative colitis	926:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	5	25	theme	higher	822:827	arg1	efficacy					829:836	higher efficacy	822:836	higher efficacy than myricetin and mesalazine	822:866	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	0	26	theme	M10	15:17	arg1	Development					0:10	Development	0:10	Development of M10	0:17	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	26	theme	M10	15:17	arg1	salt					53:56	myricetin-3-O-β-d-lactose sodium salt	20:56	myricetin-3-O-β-d-lactose sodium salt	20:56	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	26	theme	M10	15:17	arg1	derivative					61:70	a derivative	59:70	a derivative of myricetin as a potent agent of anti-chronic colonic inflammation	59:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	3	27	theme	myricetin	557:565	arg1	structure					544:552	the structure	540:552	the structure of myricetin	540:565	To solve these problems, we designed and synthesized a series of derivatives based on the structure of myricetin.					
31022552	1	28	theme	multiple	196:203	arg1	activities					205:214	multiple activities	196:214	multiple activities	196:214	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	28	theme	multiple	196:203	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	0	29	theme	sodium	46:51	arg1	Development					0:10	Development	0:10	Development of M10	0:17	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	29	theme	sodium	46:51	arg1	salt					53:56	myricetin-3-O-β-d-lactose sodium salt	20:56	myricetin-3-O-β-d-lactose sodium salt	20:56	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	30	theme	inflammation	127:138	arg1	agent					97:101	a potent agent	88:101	a potent agent of anti-chronic colonic inflammation	88:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	7	31	dep	potential	1067:1075	arg1	therapeutic					1077:1087	therapeutic	1077:1087	therapeutic	1077:1087	Based on these results, M10 could be developed as a potential therapeutic agent for treatment of ulcerative colitis.					
31022552	0	32	theme	myricetin-3-O-β-d-lactose	20:44	arg1	Development					0:10	Development	0:10	Development of M10	0:17	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	32	theme	myricetin-3-O-β-d-lactose	20:44	arg1	salt					53:56	myricetin-3-O-β-d-lactose sodium salt	20:56	myricetin-3-O-β-d-lactose sodium salt	20:56	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	0	33	theme	colonic	119:125	arg1	inflammation					127:138	anti-chronic colonic inflammation	106:138	anti-chronic colonic inflammation	106:138	Development of M10, myricetin-3-O-β-d-lactose sodium salt, a derivative of myricetin as a potent agent of anti-chronic colonic inflammation.					
31022552	7	34	theme	ulcerative	1112:1121	arg1	colitis					1123:1129	ulcerative colitis	1112:1129	ulcerative colitis	1112:1129	Based on these results, M10 could be developed as a potential therapeutic agent for treatment of ulcerative colitis.					
31022552	1	35	with	compound	182:189	arg1	activities					205:214	multiple activities	196:214	multiple activities	196:214	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	1	35	with	compound	182:189	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	5	36	theme	induced	902:908	arg1	model					915:919	a dextran sulfate sodium (DSS) induced mice model	871:919	a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis	871:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	4	37	theme	hydrophilic	597:607	arg1	group					623:627	a hydrophilic glycosylation group	595:627	a hydrophilic glycosylation group	595:627	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	4	38	theme	liver	775:779	arg1	microsomes					781:790	Wistar rat plasma and liver microsomes	753:790	microsomes	781:790	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	5	39	theme	mice	910:913	arg1	model					915:919	a dextran sulfate sodium (DSS) induced mice model	871:919	a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis	871:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	5	40	theme	sulfate	881:887	arg1	sodium					889:894	dextran sulfate sodium	873:894	a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis	871:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	5	40	theme	sulfate	881:887	arg1	DSS					897:899	DSS	897:899	DSS	897:899	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	5	41	theme	dextran	873:879	arg1	sodium					889:894	dextran sulfate sodium	873:894	a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis	871:943	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	5	41	theme	dextran	873:879	arg1	DSS					897:899	DSS	897:899	DSS	897:899	In vivo study, M10 exhibited higher efficacy than myricetin and mesalazine in a dextran sulfate sodium (DSS) induced mice model with ulcerative colitis.					
31022552	4	42	theme	sodium	648:653	arg1	derivative					660:669	a sodium salt derivative	646:669	a sodium salt derivative	646:669	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31022552	1	43	theme	anti-oxidant	225:236	arg1	effects					299:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects	225:305	Myricetin is a natural dietary flavonoid compound with multiple activities, such as anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-proliferative effects.					
31022552	7	44	theme	colitis	1123:1129	arg1	treatment					1099:1107	treatment	1099:1107	treatment of ulcerative colitis	1099:1129	Based on these results, M10 could be developed as a potential therapeutic agent for treatment of ulcerative colitis.					
31022552	3	45	theme	derivatives	519:529	arg1	series					509:514	a series	507:514	a series of derivatives based on the structure of myricetin	507:565	To solve these problems, we designed and synthesized a series of derivatives based on the structure of myricetin.					
31022552	4	46	theme	glycosylation	609:621	arg1	group					623:627	a hydrophilic glycosylation group	595:627	a hydrophilic glycosylation group	595:627	M10 was produced by adding a hydrophilic glycosylation group and then forming a sodium salt derivative, which exhibited excellent water-solubility (>100 mg/mL), and better stability in Wistar rat plasma and liver microsomes.					
31031563	4	0	theme	imaging	569:575	arg1	approach					577:584	a recently developed mass spectrometry imaging approach	530:584	a recently developed mass spectrometry imaging approach	530:584	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	2	1	theme	reflective	322:331	arg1	remnants					312:319	cellular membrane remnants	294:319	cellular membrane remnants	294:319	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	2	2	theme	regions	262:268	arg1	histopathology					235:248	The histopathology	231:248	The histopathology of necrotic regions	231:268	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	4	3	from	differences	677:687	arg1	structures					703:712	the glycan structures	692:712	the glycan structures of regions of tumor, stroma and necrosis	692:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	6	4	theme	liver	1165:1169	arg1	cancer					1171:1176	liver cancer	1165:1176	liver cancer	1165:1176	While this phenomenon was initially classified in breast cancer tissues, it has been also seen in cervical, thyroid and liver cancer samples.					
31031563	6	5	dep	cervical	1143:1150	arg1	samples					1178:1184	samples	1178:1184	samples	1178:1184	While this phenomenon was initially classified in breast cancer tissues, it has been also seen in cervical, thyroid and liver cancer samples.					
31031563	3	6	theme	progression	511:521	arg1	feature					493:499	another common feature	478:499	another common feature of cancer progression	478:521	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	1	7	theme	solid	154:158	arg1	tumors					160:165	advanced and aggressive solid tumors	130:165	advanced and aggressive solid tumors	130:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	5	8	theme	high	877:880	arg1	mannose					882:888	high mannose	877:888	high mannose	877:888	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	8	theme	high	877:880	arg1	classes					799:805	the structural glycan classes	777:805	the structural glycan classes detected in the tumor and stromal regions	777:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	4	9	theme	human	631:635	arg1	tissues					668:674	human formalin-fixed clinical cancer tissues	631:674	human formalin-fixed clinical cancer tissues	631:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	3	10	theme	common	486:491	arg1	feature					493:499	another common feature	478:499	another common feature of cancer progression	478:521	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	5	11	theme	stromal	833:839	arg1	regions					841:847	the tumor and stromal regions	819:847	regions	841:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	0	12	from	Tissues	71:77	arg1	Areas					44:48	Areas	44:48	Areas of Necrosis in Tumor Tissues	44:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	7	13	theme	necrosis	1399:1406	arg1	markers					1388:1394	prognostic markers	1377:1394	prognostic markers of necrosis	1377:1406	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	4	14	theme	clinical	652:659	arg1	tissues					668:674	human formalin-fixed clinical cancer tissues	631:674	human formalin-fixed clinical cancer tissues	631:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	4	15	theme	regions	717:723	arg1	structures					703:712	the glycan structures	692:712	the glycan structures of regions of tumor, stroma and necrosis	692:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	4	16	theme	tumor	728:732	arg1	regions					717:723	regions	717:723	regions of tumor, stroma and necrosis	717:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	1	17	from	tumors	160:165	arg1	common					120:125	common	120:125	common	120:125	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	1	18	theme	death	114:118	arg1	necrosis					87:94	Tissue necrosis	80:94	Tissue necrosis	80:94	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	1	18	theme	death	114:118	arg1	form					101:104	a form	99:104	a form of cell death common in advanced and aggressive solid tumors	99:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	7	19	theme	necrotic	1229:1236	arg1	regions					1238:1244	the necrotic regions	1225:1244	the necrotic regions	1225:1244	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	5	20	theme	tumor	823:827	arg1	regions					841:847	the tumor and stromal regions	819:847	regions	841:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	21	located	found	923:927	arg1	regions					941:947	necrotic regions	932:947	necrotic regions	932:947	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	21	located	found	923:927	arg2	glycans					915:921	the glycans	911:921	the glycans found in necrotic regions	911:947	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	1	22	from	common	120:125	arg1	tumors					160:165	advanced and aggressive solid tumors	130:165	advanced and aggressive solid tumors	130:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	2	23	theme	events	369:374	arg1	reflective					322:331	reflective	322:331	reflective	322:331	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	7	24	theme	research	1341:1348	arg1	directions					1350:1359	new research directions	1337:1359	new research directions for identifying prognostic markers of necrosis	1337:1406	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	0	25	theme	Tumor	65:69	arg1	Tissues					71:77	Tumor Tissues	65:77	Tumor Tissues	65:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	4	26	theme	stroma	735:740	arg1	regions					717:723	regions	717:723	regions of tumor, stroma and necrosis	717:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	2	27	theme	cell	352:355	arg1	degradation					357:367	cell degradation	352:367	cell degradation	352:367	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	0	28	theme	Specific	0:7	arg1	Patterns					32:39	Specific N-Linked Glycosylation Patterns	0:39	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.	0:78	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	5	29	theme	necrotic	932:939	arg1	regions					941:947	necrotic regions	932:947	necrotic regions	932:947	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	4	30	theme	necrosis	746:753	arg1	regions					717:723	regions	717:723	regions of tumor, stroma and necrosis	717:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	2	31	theme	cellular	397:404	arg1	pathway					412:418	this cellular death pathway	392:418	this cellular death pathway	392:418	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	0	32	theme	Glycosylation	18:30	arg1	Patterns					32:39	Specific N-Linked Glycosylation Patterns	0:39	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.	0:78	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	3	33	theme	cancer	504:509	arg1	progression					511:521	cancer progression	504:521	cancer progression	504:521	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	4	34	theme	developed	541:549	arg1	approach					577:584	a recently developed mass spectrometry imaging approach	530:584	a recently developed mass spectrometry imaging approach	530:584	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	5	35	theme	structural	781:790	arg1	glycans					902:908	branched glycans	893:908	branched glycans	893:908	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	35	theme	structural	781:790	arg1	mannose					882:888	high mannose	877:888	high mannose	877:888	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	35	theme	structural	781:790	arg1	classes					799:805	the structural glycan classes	777:805	the structural glycan classes detected in the tumor and stromal regions	777:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	4	36	link	N-linked	598:605	arg1	distributions					614:626	N-linked glycan distributions	598:626	N-linked glycan distributions in human formalin-fixed clinical cancer tissues	598:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	5	37	theme	fucose	1023:1028	arg1	modifications					1030:1042	fucose modifications	1023:1042	fucose modifications	1023:1042	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	7	38	from	changes	1300:1306	arg1	tissue					1318:1323	cancer tissue	1311:1323	cancer tissue	1311:1323	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	3	39	theme	proteins	466:473	arg1	glycosylation					436:448	the glycosylation	432:448	the glycosylation of cell surface proteins	432:473	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	7	40	theme	necrotic	1291:1298	arg1	changes					1300:1306	necrotic changes	1291:1306	necrotic changes in cancer tissue	1291:1323	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	3	41	theme	cell	453:456	arg1	proteins					466:473	cell surface proteins	453:473	cell surface proteins	453:473	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	5	42	theme	acid	995:998	arg1	modifications					1000:1012	sialic acid modifications	988:1012	sialic acid modifications	988:1012	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	2	43	theme	hypoxia	340:346	arg1	events					369:374	the hypoxia and cell degradation events	336:374	the hypoxia and cell degradation events associated with this cellular death pathway	336:418	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	1	44	theme	intratumoral	200:211	arg1	ischemia					221:228	intratumoral chronic ischemia	200:228	intratumoral chronic ischemia	200:228	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	5	45	theme	glycan	792:797	arg1	glycans					902:908	branched glycans	893:908	branched glycans	893:908	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	45	theme	glycan	792:797	arg1	mannose					882:888	high mannose	877:888	high mannose	877:888	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	45	theme	glycan	792:797	arg1	classes					799:805	the structural glycan classes	777:805	the structural glycan classes detected in the tumor and stromal regions	777:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	7	46	theme	further	1260:1266	arg1	insight					1280:1286	further mechanistic insight	1260:1286	further mechanistic insight to necrotic changes in cancer tissue	1260:1323	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	4	47	theme	glycan	607:612	arg1	distributions					614:626	N-linked glycan distributions	598:626	N-linked glycan distributions in human formalin-fixed clinical cancer tissues	598:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	3	48	from	Changes	421:427	arg1	glycosylation					436:448	the glycosylation	432:448	the glycosylation of cell surface proteins	432:473	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	1	49	theme	ischemia	221:228	arg1	areas					191:195	areas	191:195	areas of intratumoral chronic ischemia	191:228	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	6	50	theme	cancer	1102:1107	arg1	tissues					1109:1115	breast cancer tissues	1095:1115	breast cancer tissues	1095:1115	While this phenomenon was initially classified in breast cancer tissues, it has been also seen in cervical, thyroid and liver cancer samples.					
31031563	4	51	theme	spectrometry	556:567	arg1	approach					577:584	a recently developed mass spectrometry imaging approach	530:584	a recently developed mass spectrometry imaging approach	530:584	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	5	52	theme	branched	893:900	arg1	glycans					902:908	branched glycans	893:908	branched glycans	893:908	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	52	theme	branched	893:900	arg1	classes					799:805	the structural glycan classes	777:805	the structural glycan classes detected in the tumor and stromal regions	777:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	53	contain	contained	978:986	arg1	glycans					915:921	the glycans	911:921	the glycans found in necrotic regions	911:947	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	53	contain	contained	978:986	arg2	modifications					1000:1012	sialic acid modifications	988:1012	sialic acid modifications	988:1012	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	2	54	theme	necrotic	253:260	arg1	regions					262:268	necrotic regions	253:268	necrotic regions	253:268	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	1	55	theme	aggressive	143:152	arg1	tumors					160:165	advanced and aggressive solid tumors	130:165	advanced and aggressive solid tumors	130:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	4	56	theme	cancer	661:666	arg1	tissues					668:674	human formalin-fixed clinical cancer tissues	631:674	human formalin-fixed clinical cancer tissues	631:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	7	57	from	changes	1193:1199	arg1	glycosylation					1204:1216	glycosylation	1204:1216	glycosylation within the necrotic regions	1204:1244	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	4	58	theme	formalin-fixed	637:650	arg1	tissues					668:674	human formalin-fixed clinical cancer tissues	631:674	human formalin-fixed clinical cancer tissues	631:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	1	59	theme	cell	109:112	arg1	death					114:118	cell death	109:118	cell death	109:118	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	7	60	theme	prognostic	1377:1386	arg1	markers					1388:1394	prognostic markers	1377:1394	prognostic markers of necrosis	1377:1406	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	2	61	theme	membrane	303:310	arg1	remnants					312:319	cellular membrane remnants	294:319	cellular membrane remnants	294:319	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	4	62	from	distributions	614:626	arg1	tissues					668:674	human formalin-fixed clinical cancer tissues	631:674	human formalin-fixed clinical cancer tissues	631:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	1	63	theme	common	120:125	arg1	necrosis					87:94	Tissue necrosis	80:94	Tissue necrosis	80:94	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	1	63	theme	common	120:125	arg1	form					101:104	a form	99:104	a form of cell death common in advanced and aggressive solid tumors	99:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	0	64	from	Patterns	32:39	arg1	Areas					44:48	Areas	44:48	Areas of Necrosis in Tumor Tissues	44:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	1	65	theme	advanced	130:137	arg1	tumors					160:165	advanced and aggressive solid tumors	130:165	advanced and aggressive solid tumors	130:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	4	66	theme	glycan	696:701	arg1	structures					703:712	the glycan structures	692:712	the glycan structures of regions of tumor, stroma and necrosis	692:753	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	5	67	located	detected	807:814	arg1	regions					841:847	the tumor and stromal regions	819:847	regions	841:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	67	located	detected	807:814	arg2	mannose					882:888	high mannose	877:888	high mannose	877:888	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	67	located	detected	807:814	arg2	glycans					902:908	branched glycans	893:908	branched glycans	893:908	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	67	located	detected	807:814	arg2	classes					799:805	the structural glycan classes	777:805	the structural glycan classes detected in the tumor and stromal regions	777:847	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	5	68	theme	limited	959:965	arg1	branching					967:975	limited branching	959:975	limited branching	959:975	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	0	69	from	Necrosis	53:60	arg1	Tissues					71:77	Tumor Tissues	65:77	Tumor Tissues	65:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	3	70	theme	surface	458:464	arg1	proteins					466:473	cell surface proteins	453:473	cell surface proteins	453:473	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	7	71	theme	new	1337:1339	arg1	directions					1350:1359	new research directions	1337:1359	new research directions for identifying prognostic markers of necrosis	1337:1406	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	0	72	from	Areas	44:48	arg1	Tissues					71:77	Tumor Tissues	65:77	Tumor Tissues	65:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	2	73	theme	degradation	357:367	arg1	events					369:374	the hypoxia and cell degradation events	336:374	the hypoxia and cell degradation events associated with this cellular death pathway	336:418	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	0	74	theme	N-Linked	9:16	arg1	Patterns					32:39	Specific N-Linked Glycosylation Patterns	0:39	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.	0:78	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	1	75	theme	Tissue	80:85	arg1	necrosis					87:94	Tissue necrosis	80:94	Tissue necrosis	80:94	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	1	75	theme	Tissue	80:85	arg1	form					101:104	a form	99:104	a form of cell death common in advanced and aggressive solid tumors	99:165	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
31031563	7	76	theme	cancer	1311:1316	arg1	tissue					1318:1323	cancer tissue	1311:1323	cancer tissue	1311:1323	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	2	77	theme	death	406:410	arg1	pathway					412:418	this cellular death pathway	392:418	this cellular death pathway	392:418	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	4	78	theme	mass	551:554	arg1	approach					577:584	a recently developed mass spectrometry imaging approach	530:584	a recently developed mass spectrometry imaging approach	530:584	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	6	79	theme	breast	1095:1100	arg1	tissues					1109:1115	breast cancer tissues	1095:1115	breast cancer tissues	1095:1115	While this phenomenon was initially classified in breast cancer tissues, it has been also seen in cervical, thyroid and liver cancer samples.					
31031563	3	80	gly	glycosylation	436:448	arg1	proteins					466:473	cell surface proteins	453:473	cell surface proteins	453:473	Changes in the glycosylation of cell surface proteins is another common feature of cancer progression.					
31031563	0	81	theme	Necrosis	53:60	arg1	Areas					44:48	Areas	44:48	Areas of Necrosis in Tumor Tissues	44:77	Specific N-Linked Glycosylation Patterns in Areas of Necrosis in Tumor Tissues.					
31031563	2	82	theme	remnants	312:319	arg1	scaffold					282:289	a scaffold	280:289	a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway	280:418	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	5	83	theme	sialic	988:993	arg1	modifications					1000:1012	sialic acid modifications	988:1012	sialic acid modifications	988:1012	While the structural glycan classes detected in the tumor and stromal regions are typically classified as high mannose or branched glycans, the glycans found in necrotic regions displayed limited branching, contained sialic acid modifications and lack fucose modifications.					
31031563	7	84	theme	mechanistic	1268:1278	arg1	insight					1280:1286	further mechanistic insight	1260:1286	further mechanistic insight to necrotic changes in cancer tissue	1260:1323	These changes in glycosylation within the necrotic regions could provide further mechanistic insight to necrotic changes in cancer tissue and provide new research directions for identifying prognostic markers of necrosis.					
31031563	4	85	theme	N-linked	598:605	arg1	distributions					614:626	N-linked glycan distributions	598:626	N-linked glycan distributions in human formalin-fixed clinical cancer tissues	598:674	Using a recently developed mass spectrometry imaging approach to evaluate N-linked glycan distributions in human formalin-fixed clinical cancer tissues, differences in the glycan structures of regions of tumor, stroma and necrosis were evaluated.					
31031563	2	86	theme	cellular	294:301	arg1	remnants					312:319	cellular membrane remnants	294:319	cellular membrane remnants	294:319	The histopathology of necrotic regions appear as a scaffold of cellular membrane remnants, reflective of the hypoxia and cell degradation events associated with this cellular death pathway.					
31031563	1	87	theme	chronic	213:219	arg1	ischemia					221:228	intratumoral chronic ischemia	200:228	intratumoral chronic ischemia	200:228	Tissue necrosis is a form of cell death common in advanced and aggressive solid tumors, and is associated with areas of intratumoral chronic ischemia.					
30669833	4	0	theme	multisialylated	804:818	arg1	structures					848:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	3	1	from	patients	651:658	arg1	sera					594:597	sera	594:597	sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	594:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	6	2	from	patients	1163:1170	arg1	zone					1188:1191	the PSA gray zone	1175:1191	the PSA gray zone	1175:1191	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	6	3	theme	validation	1122:1131	arg1	set					1140:1142	an independent validation sample set	1107:1142	an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone)	1107:1192	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	6	4	theme	gray	1183:1186	arg1	zone					1188:1191	the PSA gray zone	1175:1191	the PSA gray zone	1175:1191	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	7	5	theme	PSA	1315:1317	arg1	glycoforms					1319:1328	both PSA glycoforms	1310:1328	both PSA glycoforms	1310:1328	Moreover, both PSA glycoforms showed significant correlation with Gleason scores.					
30669833	9	6	theme	grading	1680:1686	arg1	biomarker					1688:1696	a potential grading biomarker	1668:1696	a potential grading biomarker for PCa	1668:1704	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	4	7	gly	multisialylated	804:818	arg1	structures					848:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	0	8	theme	Specific	80:87	arg1	Signatures					96:105	Highly Prostate Cancer Specific Glycan Signatures	57:105	Highly Prostate Cancer Specific Glycan Signatures on PSA	57:112	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	9	9	theme	effective	1568:1576	arg1	method					1598:1603	an effective secondary screening method	1565:1603	not only an effective secondary screening method	1556:1603	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	9	9	theme	effective	1568:1576	arg1	G-index					1533:1539	PSA G-index	1529:1539	PSA G-index	1529:1539	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	3	10	theme	zone	695:698	arg1	"					699:699	the "gray zone"	685:699	the "gray zone" (4.0-10.0 ng/mL)	685:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	10	theme	zone	695:698	arg1	ng/mL					711:715	4.0-10.0 ng/mL	702:715	4.0-10.0 ng/mL	702:715	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	2	11	theme	ion	537:539	arg1	technology					552:561	mass spectrometric oxonium ion monitoring technology	510:561	mass spectrometric oxonium ion monitoring technology	510:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	6	12	theme	BPH	1159:1161	arg1	patients					1163:1170	15 PCa and 15 BPH patients	1145:1170	patients	1163:1170	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	5	13	dep	A	934:934	arg1	couple					936:941	couple	936:941	couple	936:941	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	6	14	theme	G-index	1209:1215	arg1	1.00					1221:1224	1.00	1221:1224	1.00	1221:1224	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	6	14	theme	G-index	1209:1215	arg1	AUC					1198:1200	an AUC	1195:1200	an AUC of PSA G-index	1195:1215	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	5	15	theme	PCa-specific	1018:1029	arg1	model					1041:1045	a novel PCa-specific diagnosis model	1010:1045	a novel PCa-specific diagnosis model (PSA G-index)	1010:1059	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	5	15	theme	PCa-specific	1018:1029	arg1	G-index					1052:1058	PSA G-index	1048:1058	PSA G-index	1048:1058	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	8	16	contain	containing	1476:1485	arg1	glycoproteins					1462:1474	glycoproteins	1462:1474	glycoproteins containing LacdiNAc and sialic acids moieties	1462:1520	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	8	16	contain	containing	1476:1485	arg2	moieties					1513:1520	LacdiNAc and sialic acids moieties	1487:1520	LacdiNAc and sialic acids moieties	1487:1520	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	6	17	dep	set	1140:1142	arg1	patients					1163:1170	15 PCa and 15 BPH patients	1145:1170	patients	1163:1170	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	6	17	dep	set	1140:1142	arg1	PCa					1148:1150	15 PCa and 15 BPH patients	1145:1170	PCa	1148:1150	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	3	18	theme	analysis	579:586	arg1	structures					729:738	52 glycan structures	719:738	52 glycan structures on PSA	719:745	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	18	theme	analysis	579:586	arg1	result					569:574	a result	567:574	a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	567:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	1	19	theme	antigen	139:145	arg1	test					153:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	19	theme	antigen	139:145	arg1	gold					173:176	the current gold	161:176	the current gold standard for screening and diagnosis of prostate cancer (PCa)	161:238	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	9	20	theme	false	1616:1620	arg1	diagnosis					1631:1639	false positive diagnosis	1616:1639	false positive diagnosis in PSA screening	1616:1656	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	6	21	from	PCa	1148:1150	arg1	zone					1188:1191	the PSA gray zone	1175:1191	the PSA gray zone	1175:1191	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	4	22	theme	BPH	923:925	arg1	group					927:931	the BPH group	919:931	the BPH group	919:931	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	9	23	dep	method	1598:1603	arg1	only					1560:1563	only	1560:1563	only	1560:1563	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	3	24	theme	glycan	722:727	arg1	structures					729:738	52 glycan structures	719:738	52 glycan structures on PSA	719:745	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	24	theme	glycan	722:727	arg1	result					569:574	a result	567:574	a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	567:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	6	25	theme	PSA	1254:1256	arg1	ratio					1262:1266	PSA f/T ratio	1254:1266	PSA f/T ratio	1254:1266	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	9	26	theme	PSA	1644:1646	arg1	screening					1648:1656	PSA screening	1644:1656	PSA screening	1644:1656	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	9	27	theme	positive	1622:1629	arg1	diagnosis					1631:1639	false positive diagnosis	1616:1639	false positive diagnosis in PSA screening	1616:1656	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	0	28	theme	Glycan	89:94	arg1	Signatures					96:105	Highly Prostate Cancer Specific Glycan Signatures	57:105	Highly Prostate Cancer Specific Glycan Signatures on PSA	57:112	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	2	29	theme	spectrometric	515:527	arg1	technology					552:561	mass spectrometric oxonium ion monitoring technology	510:561	mass spectrometric oxonium ion monitoring technology	510:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	8	30	theme	Lectin	1382:1387	arg1	analysis					1412:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	2	31	theme	PSA	378:380	arg1	test					382:385	PSA test	378:385	PSA test	378:385	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	5	32	theme	PSA	1048:1050	arg1	model					1041:1045	a novel PCa-specific diagnosis model	1010:1045	a novel PCa-specific diagnosis model (PSA G-index)	1010:1059	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	5	32	theme	PSA	1048:1050	arg1	G-index					1052:1058	PSA G-index	1048:1058	PSA G-index	1048:1058	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	9	33	theme	potential	1670:1678	arg1	biomarker					1688:1696	a potential grading biomarker	1668:1696	a potential grading biomarker for PCa	1668:1704	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	8	34	theme	staining	1403:1410	arg1	analysis					1412:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	2	35	theme	glycoforms	490:499	arg1	profiling					473:481	in-depth quantitative profiling	451:481	in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology	451:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	2	36	gly	glycoforms	490:499	arg1	PSA					486:488	PSA glycoforms	486:499	PSA glycoforms based on mass spectrometric oxonium ion monitoring technology	486:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	2	37	theme	glycosylation	401:413	arg1	subtypes					415:422	PCa-specific glycosylation subtypes	388:422	PCa-specific glycosylation subtypes of PSA	388:429	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	0	38	theme	Multisialylated	18:32	arg1	Structures					43:52	Multisialylated LacdiNAc Structures	18:52	Multisialylated LacdiNAc Structures	18:52	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	1	39	theme	Serum	115:119	arg1	PSA					148:150	PSA	148:150	PSA	148:150	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	39	theme	Serum	115:119	arg1	antigen					139:145	Serum prostate-specific antigen	115:145	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	2	40	theme	quantitative	460:471	arg1	profiling					473:481	in-depth quantitative profiling	451:481	in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology	451:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	3	41	theme	hyperplasia	633:643	arg1	patients					651:658	15 benign prostate hyperplasia (BPH) patients	614:658	15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	614:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	0	42	theme	Structures	43:52	arg1	Identification					0:13	Identification	0:13	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.	0:113	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	2	43	from	diagnosis	365:373	arg1	test					382:385	PSA test	378:385	PSA test	378:385	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	3	44	theme	benign	617:622	arg1	BPH					646:648	BPH	646:648	BPH	646:648	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	44	theme	benign	617:622	arg1	hyperplasia					633:643	benign prostate hyperplasia	617:643	15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	614:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	1	45	theme	prostate	218:225	arg1	PCa					235:237	PCa	235:237	PCa	235:237	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	45	theme	prostate	218:225	arg1	cancer					227:232	prostate cancer	218:232	prostate cancer (PCa)	218:238	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	9	46	from	diagnosis	1631:1639	arg1	screening					1648:1656	PSA screening	1644:1656	PSA screening	1644:1656	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	8	47	theme	LacdiNAc	1487:1494	arg1	moieties					1513:1520	LacdiNAc and sialic acids moieties	1487:1520	LacdiNAc and sialic acids moieties	1487:1520	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	2	48	theme	false	350:354	arg1	diagnosis					365:373	a false positive diagnosis	348:373	a false positive diagnosis in PSA test	348:385	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	7	49	theme	significant	1337:1347	arg1	correlation					1349:1359	significant correlation	1337:1359	significant correlation with Gleason scores	1337:1379	Moreover, both PSA glycoforms showed significant correlation with Gleason scores.					
30669833	8	50	theme	sialic	1500:1505	arg1	acids					1507:1511	sialic acids	1500:1511	sialic acids	1500:1511	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	3	51	theme	PSA	666:668	arg1	levels					670:675	levels	670:675	levels	670:675	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	9	52	theme	PSA	1529:1531	arg1	method					1598:1603	an effective secondary screening method	1565:1603	not only an effective secondary screening method	1556:1603	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	9	52	theme	PSA	1529:1531	arg1	G-index					1533:1539	PSA G-index	1529:1539	PSA G-index	1529:1539	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	7	53	with	correlation	1349:1359	arg1	scores					1374:1379	Gleason scores	1366:1379	Gleason scores	1366:1379	Moreover, both PSA glycoforms showed significant correlation with Gleason scores.					
30669833	4	54	theme	LacdiNAc	820:827	arg1	structures					848:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	9	55	theme	screening	1588:1596	arg1	method					1598:1603	an effective secondary screening method	1565:1603	not only an effective secondary screening method	1556:1603	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	9	55	theme	screening	1588:1596	arg1	G-index					1533:1539	PSA G-index	1529:1539	PSA G-index	1529:1539	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	6	56	theme	independent	1110:1120	arg1	set					1140:1142	an independent validation sample set	1107:1142	an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone)	1107:1192	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	3	57	theme	gray	690:693	arg1	"					699:699	the "gray zone"	685:699	the "gray zone" (4.0-10.0 ng/mL)	685:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	57	theme	gray	690:693	arg1	ng/mL					711:715	4.0-10.0 ng/mL	702:715	4.0-10.0 ng/mL	702:715	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	6	58	theme	PSA	1179:1181	arg1	zone					1188:1191	the PSA gray zone	1175:1191	the PSA gray zone	1175:1191	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	1	59	theme	current	165:171	arg1	test					153:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	59	theme	current	165:171	arg1	gold					173:176	the current gold	161:176	the current gold standard for screening and diagnosis of prostate cancer (PCa)	161:238	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	60	theme	standard	178:185	arg1	test					153:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	60	theme	standard	178:185	arg1	gold					173:176	the current gold	161:176	the current gold standard for screening and diagnosis of prostate cancer (PCa)	161:238	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	2	61	theme	monitoring	541:550	arg1	technology					552:561	mass spectrometric oxonium ion monitoring technology	510:561	mass spectrometric oxonium ion monitoring technology	510:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	0	62	from	Signatures	96:105	arg1	PSA					110:112	PSA	110:112	PSA	110:112	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	6	63	theme	diagnostic	1071:1080	arg1	power					1082:1086	the diagnostic power	1067:1086	the diagnostic power	1067:1086	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	5	64	theme	novel	1012:1016	arg1	model					1041:1045	a novel PCa-specific diagnosis model	1010:1045	a novel PCa-specific diagnosis model (PSA G-index)	1010:1059	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	5	64	theme	novel	1012:1016	arg1	G-index					1052:1058	PSA G-index	1048:1058	PSA G-index	1048:1058	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	2	65	theme	oxonium	529:535	arg1	technology					552:561	mass spectrometric oxonium ion monitoring technology	510:561	mass spectrometric oxonium ion monitoring technology	510:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	6	66	theme	PSA	1205:1207	arg1	G-index					1209:1215	PSA G-index	1205:1215	PSA G-index	1205:1215	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	5	67	theme	diagnosis	1031:1039	arg1	model					1041:1045	a novel PCa-specific diagnosis model	1010:1045	a novel PCa-specific diagnosis model (PSA G-index)	1010:1059	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	5	67	theme	diagnosis	1031:1039	arg1	G-index					1052:1058	PSA G-index	1048:1058	PSA G-index	1048:1058	A couple of those glycoforms were then extracted and subjected to establish a novel PCa-specific diagnosis model (PSA G-index).					
30669833	1	68	theme	prostate-specific	121:137	arg1	PSA					148:150	PSA	148:150	PSA	148:150	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	68	theme	prostate-specific	121:137	arg1	antigen					139:145	Serum prostate-specific antigen	115:145	Serum prostate-specific antigen (PSA) test	115:156	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	4	69	theme	GalNAcβ1-4GlcNAc	830:845	arg1	structures					848:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	804:857	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	4	70	theme	structures	848:857	arg1	abundance					791:799	abundance	791:799	abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures	791:857	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	6	71	theme	f/T	1258:1260	arg1	ratio					1262:1266	PSA f/T ratio	1254:1266	PSA f/T ratio	1254:1266	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	2	72	theme	PSA	486:488	arg1	glycoforms					490:499	PSA glycoforms	486:499	PSA glycoforms based on mass spectrometric oxonium ion monitoring technology	486:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	6	73	theme	sample	1133:1138	arg1	set					1140:1142	an independent validation sample set	1107:1142	an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone)	1107:1192	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	4	74	theme	PCa	897:899	arg1	group					901:905	the PCa group	893:905	the PCa group	893:905	We found that abundance of multisialylated LacdiNAc (GalNAcβ1-4GlcNAc) structures were significantly upregulated in the PCa group compared to the BPH group.					
30669833	9	75	theme	secondary	1578:1586	arg1	method					1598:1603	an effective secondary screening method	1565:1603	not only an effective secondary screening method	1556:1603	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	9	75	theme	secondary	1578:1586	arg1	G-index					1533:1539	PSA G-index	1529:1539	PSA G-index	1529:1539	Thus, PSA G-index could serve as not only an effective secondary screening method to exclude false positive diagnosis in PSA screening, but also a potential grading biomarker for PCa.					
30669833	6	76	theme	total	1241:1245	arg1	PSA					1247:1249	total PSA	1241:1249	total PSA	1241:1249	When the diagnostic power was assessed using an independent validation sample set (15 PCa and 15 BPH patients in the PSA gray zone), an AUC of PSA G-index was 1.00, while that of total PSA or PSA f/T ratio was 0.50 or 0.60, respectively.					
30669833	2	77	theme	mass	510:513	arg1	technology					552:561	mass spectrometric oxonium ion monitoring technology	510:561	mass spectrometric oxonium ion monitoring technology	510:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	8	78	theme	histochemical	1389:1401	arg1	analysis					1412:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis	1382:1419	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	3	79	from	PCa	607:609	arg1	sera					594:597	sera	594:597	sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	594:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	2	80	theme	PSA	427:429	arg1	subtypes					415:422	PCa-specific glycosylation subtypes	388:422	PCa-specific glycosylation subtypes of PSA	388:429	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	2	81	theme	positive	356:363	arg1	diagnosis					365:373	a false positive diagnosis	348:373	a false positive diagnosis in PSA test	348:385	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	0	82	theme	LacdiNAc	34:41	arg1	Structures					43:52	Multisialylated LacdiNAc Structures	18:52	Multisialylated LacdiNAc Structures	18:52	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	8	83	gly	glycoproteins	1462:1474	arg1	glycoproteins					1462:1474	glycoproteins	1462:1474	glycoproteins containing LacdiNAc and sialic acids moieties	1462:1520	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	8	84	theme	PCa	1438:1440	arg1	cells					1442:1446	PCa cells	1438:1446	PCa cells	1438:1446	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30669833	3	85	theme	prostate	624:631	arg1	BPH					646:648	BPH	646:648	BPH	646:648	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	3	85	theme	prostate	624:631	arg1	hyperplasia					633:643	benign prostate hyperplasia	617:643	15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL)	614:716	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	2	86	theme	PCa-specific	388:399	arg1	subtypes					415:422	PCa-specific glycosylation subtypes	388:422	PCa-specific glycosylation subtypes of PSA	388:429	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	1	87	theme	social	283:288	arg1	overtreatment					265:277	overtreatment	265:277	overtreatment	265:277	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	87	theme	social	283:288	arg1	problems					290:297	social problems	283:297	social problems	283:297	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	87	theme	social	283:288	arg1	overdiagnosis					247:259	overdiagnosis	247:259	overdiagnosis	247:259	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	7	88	gly	glycoforms	1319:1328	arg1	PSA					1315:1317	both PSA glycoforms	1310:1328	both PSA glycoforms	1310:1328	Moreover, both PSA glycoforms showed significant correlation with Gleason scores.					
30669833	2	89	theme	in-depth	451:458	arg1	profiling					473:481	in-depth quantitative profiling	451:481	in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology	451:561	In order to improve the specificity and exclude a false positive diagnosis in PSA test, PCa-specific glycosylation subtypes of PSA were explored using in-depth quantitative profiling of PSA glycoforms based on mass spectrometric oxonium ion monitoring technology.					
30669833	0	90	theme	Prostate	64:71	arg1	Cancer					73:78	Prostate Cancer	64:78	Prostate Cancer	64:78	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	0	91	gly	Multisialylated	18:32	arg1	Structures					43:52	Multisialylated LacdiNAc Structures	18:52	Multisialylated LacdiNAc Structures	18:52	Identification of Multisialylated LacdiNAc Structures as Highly Prostate Cancer Specific Glycan Signatures on PSA.					
30669833	3	92	from	structures	729:738	arg1	PSA					743:745	PSA	743:745	PSA	743:745	As a result of analysis using sera from 15 PCa or 15 benign prostate hyperplasia (BPH) patients whose PSA levels were in the "gray zone" (4.0-10.0 ng/mL), 52 glycan structures on PSA were quantitatively observed.					
30669833	1	93	theme	cancer	227:232	arg1	diagnosis					205:213	diagnosis	205:213	diagnosis	205:213	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	1	93	theme	cancer	227:232	arg1	screening					191:199	screening	191:199	screening	191:199	Serum prostate-specific antigen (PSA) test is the current gold standard for screening and diagnosis of prostate cancer (PCa), while overdiagnosis and overtreatment are social problems.					
30669833	7	94	theme	Gleason	1366:1372	arg1	scores					1374:1379	Gleason scores	1366:1379	Gleason scores	1366:1379	Moreover, both PSA glycoforms showed significant correlation with Gleason scores.					
30669833	8	95	theme	acids	1507:1511	arg1	moieties					1513:1520	LacdiNAc and sialic acids moieties	1487:1520	LacdiNAc and sialic acids moieties	1487:1520	Lectin histochemical staining analysis also showed that PCa cells overexpressed glycoproteins containing LacdiNAc and sialic acids moieties.					
30241071	0	0	theme	polysaccharides	76:90	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	0	0	theme	polysaccharides	76:90	arg1	activity					37:44	antioxidant activity	25:44	antioxidant activity	25:44	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	8	1	theme	Maca	1125:1128	arg1	oil					1140:1142	black Maca essential oil	1119:1142	black Maca essential oil	1119:1142	All of black Maca essential oil, lipid and polysaccharide showed higher effect than yellow and red phenotype.					
30241071	1	2	theme	Lepidium	155:162	arg1	Maca					173:176	Maca	173:176	Maca	173:176	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	1	2	theme	Lepidium	155:162	arg1	meyenii					164:170	Lepidium meyenii	155:170	Lepidium meyenii (Maca)	155:177	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	10	3	dep	analysis	1357:1364	arg1	results					1372:1378	results	1372:1378	results	1372:1378	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	1	4	theme	activities	246:255	arg1	difference					195:204	their difference	189:204	their difference of chemical compositions and biological activities	189:255	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	8	5	theme	essential	1130:1138	arg1	oil					1140:1142	black Maca essential oil	1119:1142	black Maca essential oil	1119:1142	All of black Maca essential oil, lipid and polysaccharide showed higher effect than yellow and red phenotype.					
30241071	0	6	from	analysis	12:19	arg1	phenotypes					105:114	different phenotypes	95:114	different phenotypes of Lepidium meyenii	95:134	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	1	7	theme	meyenii	164:170	arg1	phenotypes					141:150	The phenotypes	137:150	The phenotypes of Lepidium meyenii (Maca)	137:177	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	0	8	theme	different	95:103	arg1	phenotypes					105:114	different phenotypes	95:114	different phenotypes of Lepidium meyenii	95:134	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	2	9	from	components	321:330	arg1	Maca					335:338	Maca	335:338	Maca	335:338	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	6	10	theme	predominant	985:995	arg1	ingredients					997:1007	predominant ingredients	985:1007	predominant ingredients	985:1007	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	11	11	theme	quality	1549:1555	arg1	attributes					1557:1566	the quality attributes	1545:1566	the quality attributes	1545:1566	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	8	12	dep	yellow	1196:1201	arg1	phenotype					1211:1219	phenotype	1211:1219	phenotype	1211:1219	All of black Maca essential oil, lipid and polysaccharide showed higher effect than yellow and red phenotype.					
30241071	10	13	theme	essential	1397:1405	arg1	oils					1407:1410	four essential oils	1392:1410	four essential oils	1392:1410	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	3	14	theme	black	536:540	arg1	Maca					542:545	black Maca	536:545	black Maca	536:545	The essential oil, lipid and polysaccharide compositions were analyzed by GC-MS after the samples were properly pre-treated from yellow, red and black Maca.					
30241071	7	15	theme	oil	1094:1096	arg1	stronger					1102:1109	stronger	1102:1109	stronger	1102:1109	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	7	15	theme	oil	1094:1096	arg1	effect					1074:1079	the DPPH scavenging effect	1054:1079	the DPPH scavenging effect of essential oil	1054:1096	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	6	16	theme	lipid	785:789	arg1	samples					810:816	lipid and polysaccharide samples	785:816	samples	810:816	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	3	17	theme	lipid	410:414	arg1	compositions					435:446	The essential oil, lipid and polysaccharide compositions	391:446	compositions	435:446	The essential oil, lipid and polysaccharide compositions were analyzed by GC-MS after the samples were properly pre-treated from yellow, red and black Maca.					
30241071	3	18	theme	polysaccharide	420:433	arg1	compositions					435:446	The essential oil, lipid and polysaccharide compositions	391:446	compositions	435:446	The essential oil, lipid and polysaccharide compositions were analyzed by GC-MS after the samples were properly pre-treated from yellow, red and black Maca.					
30241071	3	19	theme	essential	395:403	arg1	compositions					435:446	The essential oil, lipid and polysaccharide compositions	391:446	compositions	435:446	The essential oil, lipid and polysaccharide compositions were analyzed by GC-MS after the samples were properly pre-treated from yellow, red and black Maca.					
30241071	4	20	theme	types	588:592	arg1	properties					564:573	The antioxidant properties	548:573	The antioxidant properties of the three types of components	548:606	The antioxidant properties of the three types of components were examined using the DPPH scavenging assay.					
30241071	4	21	theme	scavenging	637:646	arg1	assay					648:652	the DPPH scavenging assay	628:652	the DPPH scavenging assay	628:652	The antioxidant properties of the three types of components were examined using the DPPH scavenging assay.					
30241071	8	22	theme	higher	1177:1182	arg1	effect					1184:1189	higher effect	1177:1189	higher effect	1177:1189	All of black Maca essential oil, lipid and polysaccharide showed higher effect than yellow and red phenotype.					
30241071	0	23	from	activity	37:44	arg1	phenotypes					105:114	different phenotypes	95:114	different phenotypes of Lepidium meyenii	95:134	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	5	24	theme	statistical	742:752	arg1	method					754:759	a statistical method	740:759	a statistical method	740:759	The correlation of main compositions and the antioxidant activities was evaluated by a statistical method.					
30241071	2	25	theme	active	314:319	arg1	components					321:330	the main active components	305:330	the main active components	305:330	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	25	theme	active	314:319	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	25	theme	active	314:319	arg1	lipids					274:279	lipids	274:279	lipids	274:279	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	25	theme	active	314:319	arg1	oils					268:271	Essential oils	258:271	Essential oils	258:271	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	10	26	theme	antioxidant	1488:1498	arg1	activity					1500:1507	the antioxidant activity	1484:1507	the antioxidant activity	1484:1507	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	2	27	theme	antioxidant	371:381	arg1	effect					383:388	potential antioxidant effect	361:388	potential antioxidant effect	361:388	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	0	28	theme	Composition	0:10	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	9	29	theme	antioxidant	1298:1308	arg1	activity					1310:1317	the antioxidant activity	1294:1317	the antioxidant activity of entire Maca	1294:1332	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	6	30	from	ingredients	997:1007	arg1	which					916:920	which	916:920	which	916:920	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	31	theme	essential	770:778	arg1	oil					780:782	Maca essential oil	765:782	Maca essential oil	765:782	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	2	32	theme	main	309:312	arg1	components					321:330	the main active components	305:330	the main active components	305:330	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	32	theme	main	309:312	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	32	theme	main	309:312	arg1	lipids					274:279	lipids	274:279	lipids	274:279	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	32	theme	main	309:312	arg1	oils					268:271	Essential oils	258:271	Essential oils	258:271	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	11	33	theme	phenotypes	1615:1624	arg1	application					1590:1600	the comprehensive application	1572:1600	the comprehensive application of different phenotypes of Maca	1572:1632	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	11	33	theme	phenotypes	1615:1624	arg1	attributes					1557:1566	the quality attributes	1545:1566	the quality attributes	1545:1566	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	2	34	theme	potential	361:369	arg1	effect					383:388	potential antioxidant effect	361:388	potential antioxidant effect	361:388	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	0	35	theme	antioxidant	25:35	arg1	activity					37:44	antioxidant activity	25:44	antioxidant activity	25:44	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	9	36	theme	activity	1310:1317	arg1	%					1289:1289	about 60%	1281:1289	about 60% of the antioxidant activity of entire Maca	1281:1332	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	9	36	theme	activity	1310:1317	arg1	activity					1310:1317	the antioxidant activity	1294:1317	the antioxidant activity of entire Maca	1294:1332	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	0	37	theme	meyenii	128:134	arg1	phenotypes					105:114	different phenotypes	95:114	different phenotypes of Lepidium meyenii	95:134	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	6	38	theme	Maca	765:768	arg1	oil					780:782	Maca essential oil	765:782	Maca essential oil	765:782	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	10	39	theme	fatty	1419:1423	arg1	acids					1425:1429	three fatty acids	1413:1429	three fatty acids	1413:1429	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	11	40	theme	different	1605:1613	arg1	phenotypes					1615:1624	different phenotypes	1605:1624	different phenotypes of Maca	1605:1632	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	4	41	theme	antioxidant	552:562	arg1	properties					564:573	The antioxidant properties	548:573	The antioxidant properties of the three types of components	548:606	The antioxidant properties of the three types of components were examined using the DPPH scavenging assay.					
30241071	0	42	theme	Lepidium	119:126	arg1	meyenii					128:134	Lepidium meyenii	119:134	Lepidium meyenii	119:134	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	7	43	theme	essential	1084:1092	arg1	oil					1094:1096	essential oil	1084:1096	essential oil	1084:1096	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	3	44	theme	oil	405:407	arg1	compositions					435:446	The essential oil, lipid and polysaccharide compositions	391:446	compositions	435:446	The essential oil, lipid and polysaccharide compositions were analyzed by GC-MS after the samples were properly pre-treated from yellow, red and black Maca.					
30241071	6	45	theme	fatty	956:960	arg1	acids					962:966	unsaturation fatty acids	943:966	unsaturation fatty acids	943:966	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	5	46	theme	antioxidant	700:710	arg1	activities					712:721	the antioxidant activities	696:721	the antioxidant activities	696:721	The correlation of main compositions and the antioxidant activities was evaluated by a statistical method.					
30241071	6	47	theme	fatty	858:862	arg1	acids					864:868	twenty-nine fatty acids	846:868	twenty-nine fatty acids	846:868	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	4	48	theme	DPPH	632:635	arg1	assay					648:652	the DPPH scavenging assay	628:652	the DPPH scavenging assay	628:652	The antioxidant properties of the three types of components were examined using the DPPH scavenging assay.					
30241071	6	49	theme	unsaturation	943:954	arg1	acids					962:966	unsaturation fatty acids	943:966	unsaturation fatty acids	943:966	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	5	50	theme	activities	712:721	arg1	correlation					659:669	The correlation	655:669	The correlation of main compositions and the antioxidant activities	655:721	The correlation of main compositions and the antioxidant activities was evaluated by a statistical method.					
30241071	6	51	theme	twenty-nine	846:856	arg1	acids					864:868	twenty-nine fatty acids	846:868	twenty-nine fatty acids	846:868	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	52	located	detected	903:910	arg1	oil					780:782	Maca essential oil	765:782	Maca essential oil	765:782	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	52	located	detected	903:910	arg2	acids					864:868	twenty-nine fatty acids	846:868	twenty-nine fatty acids	846:868	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	52	located	detected	903:910	arg2	samples					810:816	lipid and polysaccharide samples	785:816	samples	810:816	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	52	located	detected	903:910	arg2	compounds					835:843	thirty volatile compounds	819:843	thirty volatile compounds	819:843	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	6	52	located	detected	903:910	arg2	monosaccharides					878:892	six monosaccharides	874:892	six monosaccharides	874:892	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	0	53	theme	oils	59:62	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	0	53	theme	oils	59:62	arg1	activity					37:44	antioxidant activity	25:44	antioxidant activity	25:44	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	9	54	theme	Maca	1329:1332	arg1	activity					1310:1317	the antioxidant activity	1294:1317	the antioxidant activity of entire Maca	1294:1332	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	7	55	theme	components	1042:1051	arg1	types					1033:1037	three types	1027:1037	three types of components	1027:1051	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	7	56	theme	DPPH	1058:1061	arg1	stronger					1102:1109	stronger	1102:1109	stronger	1102:1109	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	7	56	theme	DPPH	1058:1061	arg1	effect					1074:1079	the DPPH scavenging effect	1054:1079	the DPPH scavenging effect of essential oil	1054:1096	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	0	57	theme	essential	49:57	arg1	oils					59:62	essential oils	49:62	essential oils	49:62	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	7	58	theme	scavenging	1063:1072	arg1	stronger					1102:1109	stronger	1102:1109	stronger	1102:1109	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	7	58	theme	scavenging	1063:1072	arg1	effect					1074:1079	the DPPH scavenging effect	1054:1079	the DPPH scavenging effect of essential oil	1054:1096	In three types of components, the DPPH scavenging effect of essential oil was stronger.					
30241071	9	59	theme	components	1263:1272	arg1	types					1254:1258	the three types	1244:1258	the three types of components	1244:1272	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	9	60	theme	total	1226:1230	arg1	activity					1232:1239	The total activity	1222:1239	The total activity of the three types of components	1222:1272	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	5	61	theme	main	674:677	arg1	compositions					679:690	main compositions	674:690	main compositions	674:690	The correlation of main compositions and the antioxidant activities was evaluated by a statistical method.					
30241071	6	62	theme	volatile	826:833	arg1	compounds					835:843	thirty volatile compounds	819:843	thirty volatile compounds	819:843	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	9	63	theme	entire	1322:1327	arg1	Maca					1329:1332	entire Maca	1322:1332	entire Maca	1322:1332	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	6	64	theme	polysaccharide	795:808	arg1	samples					810:816	lipid and polysaccharide samples	785:816	samples	810:816	In Maca essential oil, lipid and polysaccharide samples, thirty volatile compounds, twenty-nine fatty acids and six monosaccharides had been detected, in which benzeneacetonitrile, unsaturation fatty acids and mannose were predominant ingredients, respectively.					
30241071	1	65	theme	chemical	209:216	arg1	compositions					218:229	chemical compositions	209:229	chemical compositions	209:229	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	5	66	theme	compositions	679:690	arg1	correlation					659:669	The correlation	655:669	The correlation of main compositions and the antioxidant activities	655:721	The correlation of main compositions and the antioxidant activities was evaluated by a statistical method.					
30241071	2	67	theme	Essential	258:266	arg1	components					321:330	the main active components	305:330	the main active components	305:330	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	67	theme	Essential	258:266	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	67	theme	Essential	258:266	arg1	lipids					274:279	lipids	274:279	lipids	274:279	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	2	67	theme	Essential	258:266	arg1	oils					268:271	Essential oils	258:271	Essential oils	258:271	Essential oils, lipids and polysaccharides are the main active components in Maca, all of which showed potential antioxidant effect.					
30241071	10	68	theme	correlation	1345:1355	arg1	CCA					1367:1369	CCA	1367:1369	CCA	1367:1369	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	10	68	theme	correlation	1345:1355	arg1	analysis					1357:1364	Canonical correlation analysis	1335:1364	Canonical correlation analysis (CCA) results	1335:1378	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	1	69	theme	compositions	218:229	arg1	difference					195:204	their difference	189:204	their difference of chemical compositions and biological activities	189:255	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
30241071	0	70	theme	lipids	65:70	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	0	70	theme	lipids	65:70	arg1	activity					37:44	antioxidant activity	25:44	antioxidant activity	25:44	Composition analysis and antioxidant activity of essential oils, lipids and polysaccharides in different phenotypes of Lepidium meyenii.					
30241071	9	71	theme	types	1254:1258	arg1	activity					1232:1239	The total activity	1222:1239	The total activity of the three types of components	1222:1272	The total activity of the three types of components showed about 60% of the antioxidant activity of entire Maca.					
30241071	10	72	theme	Canonical	1335:1343	arg1	CCA					1367:1369	CCA	1367:1369	CCA	1367:1369	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	10	72	theme	Canonical	1335:1343	arg1	analysis					1357:1364	Canonical correlation analysis	1335:1364	Canonical correlation analysis (CCA) results	1335:1378	Canonical correlation analysis (CCA) results showed that four essential oils, three fatty acids and four monosaccharaides were positively related to the antioxidant activity.					
30241071	8	73	theme	black	1119:1123	arg1	oil					1140:1142	black Maca essential oil	1119:1142	black Maca essential oil	1119:1142	All of black Maca essential oil, lipid and polysaccharide showed higher effect than yellow and red phenotype.					
30241071	11	74	theme	Maca	1629:1632	arg1	phenotypes					1615:1624	different phenotypes	1605:1624	different phenotypes of Maca	1605:1632	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	4	75	theme	components	597:606	arg1	types					588:592	the three types	578:592	the three types of components	578:606	The antioxidant properties of the three types of components were examined using the DPPH scavenging assay.					
30241071	11	76	theme	comprehensive	1576:1588	arg1	application					1590:1600	the comprehensive application	1572:1600	the comprehensive application of different phenotypes of Maca	1572:1632	The research is helpful to address the quality attributes and the comprehensive application of different phenotypes of Maca.					
30241071	1	77	theme	biological	235:244	arg1	activities					246:255	biological activities	235:255	biological activities	235:255	The phenotypes of Lepidium meyenii (Maca) relate to their difference of chemical compositions and biological activities.					
31924008	7	0	theme	infection	1527:1535	arg1	prevention					1537:1546	infection prevention	1527:1546	infection prevention	1527:1546	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	7	0	theme	infection	1527:1535	arg1	materials					1496:1504	materials biocompatibility and infection prevention	1496:1546	materials biocompatibility and infection prevention	1496:1546	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	4	1	theme	composites	832:841	arg1	features					815:822	biological features	804:822	biological features	804:822	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	4	1	theme	composites	832:841	arg1	morphology					777:786	morphology	777:786	morphology	777:786	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	4	1	theme	composites	832:841	arg1	composition					764:774	surface composition	756:774	surface composition	756:774	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	6	2	theme	preventive	1326:1335	arg1	activity					1337:1344	antibacterial preventive activity	1312:1344	antibacterial preventive activity	1312:1344	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	3	3	theme	Manuka	646:651	arg1	gum					627:629	gellan gum	620:629	gellan gum	620:629	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	3	theme	Manuka	646:651	arg1	honey					653:657	antibacterial Manuka honey	632:657	antibacterial Manuka honey	632:657	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	6	4	theme	in	1281:1282	arg1	cytocompatibility					1290:1306	in vitro cytocompatibility	1281:1306	in vitro cytocompatibility	1281:1306	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	7	5	theme	subcutaneous	1447:1458	arg1	implantation					1460:1471	subcutaneous implantation	1447:1471	subcutaneous implantation in mice	1447:1479	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	5	6	with	incubation	996:1005	arg1	cells					1035:1039	human mesenchymal stem cells	1012:1039	human mesenchymal stem cells	1012:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	1	7	theme	limited	262:268	arg1	capacity					292:299	the tissue's limited intrinsic self-repair capacity	249:299	the tissue's limited intrinsic self-repair capacity	249:299	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	4	8	theme	biological	804:813	arg1	features					815:822	biological features	804:822	biological features	804:822	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	3	9	theme	inorganic	666:674	arg1	clay					676:679	an inorganic clay	663:679	an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes)	663:749	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	9	theme	inorganic	666:674	arg1	gum					627:629	gellan gum	620:629	gellan gum	620:629	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	9	10	theme	promising	1726:1734	arg1	tool					1736:1739	a very promising tool	1719:1739	a very promising tool for cartilage tissue engineering	1719:1772	Accordingly, GG-MH-MS composites represent a very promising tool for cartilage tissue engineering.					
31924008	1	11	theme	intrinsic	270:278	arg1	capacity					292:299	the tissue's limited intrinsic self-repair capacity	249:299	the tissue's limited intrinsic self-repair capacity	249:299	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	0	12	theme	honey	143:147	arg1	hydrogels					149:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	3	13	theme	sodium‑calcium	701:714	arg1	bentonite					716:724	sodium‑calcium bentonite	701:724	sodium‑calcium bentonite	701:724	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	1	14	theme	defects	193:199	arg1	treatment					170:178	Effective treatment	160:178	Effective treatment of cartilage defects	160:199	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	9	15	theme	tissue	1755:1760	arg1	engineering					1762:1772	cartilage tissue engineering	1745:1772	cartilage tissue engineering	1745:1772	Accordingly, GG-MH-MS composites represent a very promising tool for cartilage tissue engineering.					
31924008	0	16	theme	Gellan	125:130	arg1	hydrogels					149:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	1	17	theme	self-repair	280:290	arg1	capacity					292:299	the tissue's limited intrinsic self-repair capacity	249:299	the tissue's limited intrinsic self-repair capacity	249:299	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	6	18	theme	mesoporous	1220:1229	arg1	silica					1231:1236	mesoporous silica	1220:1236	mesoporous silica	1220:1236	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	6	19	theme	antibacterial	1312:1324	arg1	activity					1337:1344	antibacterial preventive activity	1312:1344	antibacterial preventive activity	1312:1344	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	6	20	theme	cytocompatibility	1290:1306	arg1	terms					1272:1276	terms	1272:1276	terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model	1272:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	1	21	theme	poor	404:407	arg1	properties					420:429	poor mechanical properties	404:429	poor mechanical properties	404:429	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	0	22	theme	inorganic	47:55	arg1	clays					57:61	inorganic clays	47:61	inorganic clays	47:61	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	1	23	theme	scaffolds	323:331	arg1	use					306:308	the use	302:308	the use of polymeric scaffolds as tissue	302:341	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	5	24	theme	mesenchymal	1018:1028	arg1	cells					1035:1039	human mesenchymal stem cells	1012:1039	human mesenchymal stem cells	1012:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	1	25	theme	Effective	160:168	arg1	treatment					170:178	Effective treatment	160:178	Effective treatment of cartilage defects	160:199	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	4	26	theme	superior	904:911	arg1	scaffold					913:920	a superior scaffold	902:920	a superior scaffold for cartilage repair	902:941	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	0	27	from	Advances	0:7	arg1	repair					22:27	cartilage repair	12:27	cartilage repair	12:27	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	0	28	theme	mechanical	74:83	arg1	properties					97:106	mechanical and healing properties	74:106	properties	97:106	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	5	29	theme	silica-composite	1057:1072	arg1	hydrogels					1074:1082	the mesoporous silica-composite hydrogels	1042:1082	the mesoporous silica-composite hydrogels	1042:1082	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	2	30	theme	healing	496:502	arg1	process					504:510	the healing process	492:510	the healing process	492:510	Moreover, the onset of an infection can irreversibly affect the healing process.					
31924008	0	31	theme	healing	89:95	arg1	properties					97:106	mechanical and healing properties	74:106	properties	97:106	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	5	32	theme	superior	1178:1185	arg1	properties					1198:1207	superior mechanical properties	1178:1207	superior mechanical properties	1178:1207	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	7	33	dep	materials	1496:1504	arg1	biocompatibility					1506:1521	biocompatibility	1506:1521	biocompatibility	1506:1521	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	7	33	dep	materials	1496:1504	arg1	prevention					1537:1546	infection prevention	1527:1546	infection prevention	1527:1546	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	7	33	dep	materials	1496:1504	arg1	materials					1496:1504	materials biocompatibility and infection prevention	1496:1546	materials biocompatibility and infection prevention	1496:1546	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	1	34	theme	mechanical	409:418	arg1	properties					420:429	poor mechanical properties	404:429	poor mechanical properties	404:429	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	0	35	theme	cartilage	12:20	arg1	repair					22:27	cartilage repair	12:27	cartilage repair	12:27	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	8	36	theme	immune	1607:1612	arg1	response					1614:1621	severe immune response	1600:1621	severe immune response	1600:1621	Results demonstrated that composites did not cause severe immune response as well as they were able to restrain the infection.					
31924008	5	37	theme	in	987:988	arg1	incubation					996:1005	in vitro incubation	987:1005	in vitro incubation with human mesenchymal stem cells	987:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	3	38	theme	scaffolds	601:609	arg1	preparation					576:586	the preparation	572:586	the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes)	572:749	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	5	39	theme	incubation	996:1005	arg1	45 days					976:982	45 days	976:982	45 days of in vitro incubation with human mesenchymal stem cells	976:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	3	40	theme	antibacterial	632:644	arg1	gum					627:629	gellan gum	620:629	gellan gum	620:629	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	40	theme	antibacterial	632:644	arg1	honey					653:657	antibacterial Manuka honey	632:657	antibacterial Manuka honey	632:657	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	5	41	theme	dynamic	1134:1140	arg1	moduli					1142:1147	dynamic moduli	1134:1147	dynamic moduli	1134:1147	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	0	42	dep	Advances	0:7	arg1	influence					34:42	The influence	30:42	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.	0:158	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	6	43	from	cytocompatibility	1290:1306	arg1	protection					1349:1358	protection	1349:1358	protection of cells in a co-culture model	1349:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	5	44	theme	mechanical	1187:1196	arg1	properties					1198:1207	superior mechanical properties	1178:1207	superior mechanical properties	1178:1207	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	4	45	theme	such	827:830	arg1	composites					832:841	such composites	827:841	such composites	827:841	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	3	46	theme	mesoporous	682:691	arg1	silica					693:698	mesoporous silica	682:698	mesoporous silica	682:698	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	4	47	dep	composition	764:774	arg1	The					752:754	The	752:754	The	752:754	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	6	48	dep	in	1281:1282	arg1	vitro					1284:1288	vitro	1284:1288	vitro	1284:1288	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	6	49	theme	best	1251:1254	arg1	performances					1256:1267	the best performances	1247:1267	the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model	1247:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	1	50	theme	cartilage	183:191	arg1	defects					193:199	cartilage defects	183:199	cartilage defects	183:199	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	5	51	dep	in	987:988	arg1	vitro					990:994	vitro	990:994	vitro	990:994	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	0	52	theme	antibacterial	111:123	arg1	hydrogels					149:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	9	53	theme	cartilage	1745:1753	arg1	engineering					1762:1772	cartilage tissue engineering	1745:1772	cartilage tissue engineering	1745:1772	Accordingly, GG-MH-MS composites represent a very promising tool for cartilage tissue engineering.					
31924008	7	54	theme	selected	1408:1415	arg1	composition					1417:1427	this selected composition	1403:1427	this selected composition	1403:1427	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	0	55	theme	gum-Manuka	132:141	arg1	hydrogels					149:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	antibacterial Gellan gum-Manuka honey hydrogels	111:157	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	3	56	theme	first	560:564	arg1	time					566:569	the first time	556:569	the first time	556:569	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	1	57	theme	challenging	214:224	arg1	problem					226:232	a challenging problem	212:232	a challenging problem	212:232	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	0	58	theme	hydrogels	149:157	arg1	properties					97:106	mechanical and healing properties	74:106	properties	97:106	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	5	59	theme	mesoporous	1046:1055	arg1	hydrogels					1074:1082	the mesoporous silica-composite hydrogels	1042:1082	the mesoporous silica-composite hydrogels	1042:1082	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	6	60	from	performances	1256:1267	arg1	terms					1272:1276	terms	1272:1276	terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model	1272:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	3	61	theme	halloysite	729:738	arg1	nanotubes					740:748	halloysite nanotubes	729:748	halloysite nanotubes	729:748	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	5	62	theme	human	1012:1016	arg1	cells					1035:1039	human mesenchymal stem cells	1012:1039	human mesenchymal stem cells	1012:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	0	63	theme	clays	57:61	arg1	influence					34:42	The influence	30:42	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.	0:158	Advances in cartilage repair: The influence of inorganic clays to improve mechanical and healing properties of antibacterial Gellan gum-Manuka honey hydrogels.					
31924008	5	64	theme	stem	1030:1033	arg1	cells					1035:1039	human mesenchymal stem cells	1012:1039	human mesenchymal stem cells	1012:1039	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	4	65	theme	cartilage	926:934	arg1	repair					936:941	cartilage repair	926:941	cartilage repair	926:941	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	6	66	from	model	1385:1389	arg1	protection					1349:1358	protection	1349:1358	protection of cells in a co-culture model	1349:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	2	67	theme	infection	458:466	arg1	onset					446:450	the onset	442:450	the onset of an infection	442:466	Moreover, the onset of an infection can irreversibly affect the healing process.					
31924008	6	68	theme	co-culture	1374:1383	arg1	model					1385:1389	a co-culture model	1372:1389	a co-culture model	1372:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	7	69	from	implantation	1460:1471	arg1	mice					1476:1479	mice	1476:1479	mice	1476:1479	Therefore, this selected composition was exploited for subcutaneous implantation in mice to investigate materials biocompatibility and infection prevention.					
31924008	6	70	from	cells	1363:1367	arg1	model					1385:1389	a co-culture model	1372:1389	a co-culture model	1372:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	4	71	theme	scaffold	913:920	arg1	composition					887:897	the composition	883:897	the composition of a superior scaffold for cartilage repair	883:941	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
31924008	5	72	theme	significant	1094:1104	arg1	changes					1106:1112	significant changes	1094:1112	significant changes in peak elastic and dynamic moduli	1094:1147	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	9	73	theme	GG-MH-MS	1689:1696	arg1	composites					1698:1707	GG-MH-MS composites	1689:1707	GG-MH-MS composites	1689:1707	Accordingly, GG-MH-MS composites represent a very promising tool for cartilage tissue engineering.					
31924008	8	74	theme	severe	1600:1605	arg1	response					1614:1621	severe immune response	1600:1621	severe immune response	1600:1621	Results demonstrated that composites did not cause severe immune response as well as they were able to restrain the infection.					
31924008	6	75	from	activity	1337:1344	arg1	protection					1349:1358	protection	1349:1358	protection of cells in a co-culture model	1349:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	6	76	from	protection	1349:1358	arg1	model					1385:1389	a co-culture model	1372:1389	a co-culture model	1372:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	6	76	from	protection	1349:1358	arg1	terms					1272:1276	terms	1272:1276	terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model	1272:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	3	77	theme	composite	591:599	arg1	scaffolds					601:609	composite scaffolds	591:609	composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes)	591:749	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	6	78	theme	cells	1363:1367	arg1	protection					1349:1358	protection	1349:1358	protection of cells in a co-culture model	1349:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	5	79	from	changes	1106:1112	arg1	moduli					1142:1147	dynamic moduli	1134:1147	dynamic moduli	1134:1147	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	5	79	from	changes	1106:1112	arg1	elastic					1122:1128	elastic	1122:1128	elastic	1122:1128	Results demonstrated that after 45 days of in vitro incubation with human mesenchymal stem cells, the mesoporous silica-composite hydrogels exhibited significant changes in peak elastic and dynamic moduli over time thus demonstrating superior mechanical properties.					
31924008	3	80	dep	clay	676:679	arg1	silica					693:698	mesoporous silica	682:698	mesoporous silica	682:698	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	80	dep	clay	676:679	arg1	bentonite					716:724	sodium‑calcium bentonite	701:724	sodium‑calcium bentonite	701:724	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	80	dep	clay	676:679	arg1	nanotubes					740:748	halloysite nanotubes	729:748	halloysite nanotubes	729:748	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	6	81	theme	activity	1337:1344	arg1	terms					1272:1276	terms	1272:1276	terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model	1272:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	1	82	theme	polymeric	313:321	arg1	scaffolds					323:331	polymeric scaffolds	313:331	polymeric scaffolds	313:331	Effective treatment of cartilage defects represents a challenging problem, mainly due to the tissue's limited intrinsic self-repair capacity; the use of polymeric scaffolds as tissue substitute is rapidly increasing, but it is still limited by poor mechanical properties.					
31924008	3	83	theme	gellan	620:625	arg1	gum					627:629	gellan gum	620:629	gellan gum	620:629	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	83	theme	gellan	620:625	arg1	honey					653:657	antibacterial Manuka honey	632:657	antibacterial Manuka honey	632:657	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	3	83	theme	gellan	620:625	arg1	clay					676:679	an inorganic clay	663:679	an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes)	663:749	Accordingly, in this work we describe, for the first time, the preparation of composite scaffolds based on gellan gum, antibacterial Manuka honey and an inorganic clay (mesoporous silica, sodium‑calcium bentonite or halloysite nanotubes).					
31924008	6	84	from	terms	1272:1276	arg1	protection					1349:1358	protection	1349:1358	protection of cells in a co-culture model	1349:1389	Moreover, mesoporous silica provided the best performances in terms of in vitro cytocompatibility and antibacterial preventive activity in protection of cells in a co-culture model.					
31924008	4	85	theme	surface	756:762	arg1	composition					764:774	surface composition	756:774	surface composition	756:774	The surface composition, morphology, mechanical and biological features of such composites are herein assessed, aiming to optimize the composition of a superior scaffold for cartilage repair.					
30638505	6	0	theme	2→6	927:929	arg1	-puerarin					931:939	fructosyl-β-(2→6)-puerarin	914:939	fructosyl-β-(2→6)-puerarin	914:939	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
30638505	1	1	theme	compounds	187:195	arg1	bioactivity					163:173	bioactivity	163:173	bioactivity	163:173	Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits.					
30638505	1	1	theme	compounds	187:195	arg1	stability					149:157	stability	149:157	stability	149:157	Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits.					
30638505	1	1	theme	compounds	187:195	arg1	solubility					137:146	solubility	137:146	solubility	137:146	Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits.					
30638505	3	2	theme	conversion	548:557	arg1	rates					559:563	conversion rates	548:563	conversion rates of 93% and 25.1%, respectively	548:594	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	6	3	theme	fructosyl-β-	914:925	arg1	-puerarin					931:939	fructosyl-β-(2→6)-puerarin	914:939	fructosyl-β-(2→6)-puerarin	914:939	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
30638505	2	4	theme	phenolic	379:386	arg1	compounds					388:396	phenolic compounds	379:396	phenolic compounds	379:396	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	2	5	theme	compounds	388:396	arg1	classes					368:374	different classes	358:374	different classes of phenolic compounds	358:396	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	4	6	theme	32:8:1	686:691	arg1	ratio					665:669	a ratio	663:669	a ratio of 37:14:1 and 32:8:1, respectively	663:705	In both cases, mono-, di-, and trifructosides were synthesized at a ratio of 37:14:1 and 32:8:1, respectively.					
30638505	2	7	from	diazotrophicus	268:281	arg1	Levansucrase					232:243	Levansucrase	232:243	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10)	232:301	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	3	8	theme	phenol	467:472	arg1	compounds					532:540	the most efficiently fructosylated compounds	497:540	the most efficiently fructosylated compounds	497:540	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	8	theme	phenol	467:472	arg1	alcohol					484:490	the phenol coniferyl alcohol	463:490	the phenol coniferyl alcohol	463:490	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	8	theme	phenol	467:472	arg1	puerarin					450:457	the isoflavone puerarin	435:457	the isoflavone puerarin	435:457	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	5	9	theme	mono-fructoside	752:766	arg1	characterization					719:734	Structural characterization	708:734	Structural characterization of the puerarin mono-fructoside	708:766	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	6	10	theme	water	894:898	arg1	solubility					900:909	The water solubility	890:909	The water solubility of fructosyl-β-(2→6)-puerarin	890:939	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
30638505	3	11	theme	%	570:570	arg1	rates					559:563	conversion rates	548:563	conversion rates of 93% and 25.1%, respectively	548:594	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	12	theme	coniferyl	474:482	arg1	compounds					532:540	the most efficiently fructosylated compounds	497:540	the most efficiently fructosylated compounds	497:540	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	12	theme	coniferyl	474:482	arg1	alcohol					484:490	the phenol coniferyl alcohol	463:490	the phenol coniferyl alcohol	463:490	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	12	theme	coniferyl	474:482	arg1	puerarin					450:457	the isoflavone puerarin	435:457	the isoflavone puerarin	435:457	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	13	with	compounds	532:540	arg1	rates					559:563	conversion rates	548:563	conversion rates of 93% and 25.1%, respectively	548:594	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	2	14	theme	sucrose	347:353	arg1	unit					339:342	the fructosyl unit	325:342	the fructosyl unit of sucrose	325:353	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	1	15	theme	health	215:220	arg1	benefits					222:229	their health benefits	209:229	their health benefits	209:229	Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits.					
30638505	5	16	theme	sucrose	829:835	arg1	moiety					819:824	the fructosyl moiety	805:824	the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin	805:887	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	0	17	theme	compounds	27:35	arg1	Fructosylation					0:13	Fructosylation	0:13	Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.	0:90	Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.					
30638505	3	18	theme	%	580:580	arg1	rates					559:563	conversion rates	548:563	conversion rates of 93% and 25.1%, respectively	548:594	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	0	19	theme	phenolic	18:25	arg1	compounds					27:35	phenolic compounds	18:35	phenolic compounds	18:35	Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.					
30638505	2	20	theme	fructosyl	329:337	arg1	unit					339:342	the fructosyl unit	325:342	the fructosyl unit of sucrose	325:353	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	3	21	theme	isoflavone	439:448	arg1	compounds					532:540	the most efficiently fructosylated compounds	497:540	the most efficiently fructosylated compounds	497:540	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	21	theme	isoflavone	439:448	arg1	alcohol					484:490	the phenol coniferyl alcohol	463:490	the phenol coniferyl alcohol	463:490	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	21	theme	isoflavone	439:448	arg1	puerarin					450:457	the isoflavone puerarin	435:457	the isoflavone puerarin	435:457	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	5	22	theme	Structural	708:717	arg1	characterization					719:734	Structural characterization	708:734	Structural characterization of the puerarin mono-fructoside	708:766	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	5	23	theme	unit	872:875	arg1	O6-position					844:854	the O6-position	840:854	the O6-position of the glucosyl unit of puerarin	840:887	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	5	24	theme	puerarin	880:887	arg1	unit					872:875	the glucosyl unit	859:875	the glucosyl unit of puerarin	859:887	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	2	25	theme	LsdA	284:287	arg1	diazotrophicus					268:281	Gluconacetobacter diazotrophicus	250:281	Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10)	250:301	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	2	25	theme	LsdA	284:287	arg1	EC					290:291	LsdA, EC 2.4.1.10	284:300	EC	290:291	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	5	26	theme	glucosyl	863:870	arg1	unit					872:875	the glucosyl unit	859:875	the glucosyl unit of puerarin	859:887	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	0	27	theme	Gluconacetobacter	58:74	arg1	diazotrophicus					76:89	Gluconacetobacter diazotrophicus	58:89	Gluconacetobacter diazotrophicus	58:89	Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.					
30638505	0	28	from	diazotrophicus	76:89	arg1	levansucrase					40:51	levansucrase	40:51	levansucrase from Gluconacetobacter diazotrophicus	40:89	Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.					
30638505	2	29	theme	Gluconacetobacter	250:266	arg1	diazotrophicus					268:281	Gluconacetobacter diazotrophicus	250:281	Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10)	250:301	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	2	29	theme	Gluconacetobacter	250:266	arg1	EC					290:291	LsdA, EC 2.4.1.10	284:300	EC	290:291	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	6	30	theme	-puerarin	931:939	arg1	solubility					900:909	The water solubility	890:909	The water solubility of fructosyl-β-(2→6)-puerarin	890:939	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
30638505	4	31	theme	37:14:1	674:680	arg1	ratio					665:669	a ratio	663:669	a ratio of 37:14:1 and 32:8:1, respectively	663:705	In both cases, mono-, di-, and trifructosides were synthesized at a ratio of 37:14:1 and 32:8:1, respectively.					
30638505	2	32	theme	different	358:366	arg1	classes					368:374	different classes	358:374	different classes of phenolic compounds	358:396	Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds.					
30638505	5	33	theme	puerarin	743:750	arg1	mono-fructoside					752:766	the puerarin mono-fructoside	739:766	the puerarin mono-fructoside	739:766	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	6	34	dep	16.2 g	970:975	arg1	to					967:968	to	967:968	to	967:968	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
30638505	3	35	theme	fructosylated	518:530	arg1	compounds					532:540	the most efficiently fructosylated compounds	497:540	the most efficiently fructosylated compounds	497:540	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	35	theme	fructosylated	518:530	arg1	alcohol					484:490	the phenol coniferyl alcohol	463:490	the phenol coniferyl alcohol	463:490	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	3	35	theme	fructosylated	518:530	arg1	puerarin					450:457	the isoflavone puerarin	435:457	the isoflavone puerarin	435:457	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	5	36	theme	fructosyl	809:817	arg1	moiety					819:824	the fructosyl moiety	805:824	the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin	805:887	Structural characterization of the puerarin mono-fructoside revealed that the enzyme transferred the fructosyl moiety of sucrose to the O6-position of the glucosyl unit of puerarin.					
30638505	3	37	theme	various	409:415	arg1	acceptors					417:425	the various acceptors	405:425	the various acceptors tested	405:432	Among the various acceptors tested, the isoflavone puerarin and the phenol coniferyl alcohol were the most efficiently fructosylated compounds, with conversion rates of 93% and 25.1%, respectively.					
30638505	1	38	theme	phenolic	178:185	arg1	compounds					187:195	phenolic compounds	178:195	phenolic compounds	178:195	Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits.					
30638505	6	39	theme	antioxidant	992:1002	arg1	capacity					1004:1011	its antioxidant capacity	988:1011	its antioxidant capacity	988:1011	The water solubility of fructosyl-β-(2→6)-puerarin was increased 23-fold, up to 16.2 g L-1, while its antioxidant capacity was only decreased 1.25-fold compared with that of puerarin.					
31004636	0	0	theme	Prunus	81:86	arg1	polysaccharide					103:116	Prunus cerasifera gum polysaccharide	81:116	Prunus cerasifera gum polysaccharide	81:116	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	3	1	theme	acids	920:924	arg1	mannose					901:907	mannose	901:907	mannose	901:907	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	1	theme	acids	920:924	arg1	acids					920:924	uronic acids	913:924	uronic acids	913:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	1	theme	acids	920:924	arg1	xylose					893:898	xylose	893:898	xylose	893:898	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	1	theme	acids	920:924	arg1	amount					883:888	minor amount	877:888	minor amount of xylose, mannose and uronic acids	877:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	2	2	theme	tree	368:371	arg1	gum					373:375	Prunus cerasifera tree gum	350:375	Prunus cerasifera tree gum	350:375	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	3	3	theme	cerasifera	782:791	arg1	gum					798:800	Prunus cerasifera tree gum	775:800	Prunus cerasifera tree gum	775:800	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	2	4	theme	high-performance	396:411	arg1	HPAEC					444:448	HPAEC	444:448	HPAEC	444:448	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	4	theme	high-performance	396:411	arg1	chromatography					428:441	a high-performance anion exchange chromatography	394:441	a high-performance anion exchange chromatography (HPAEC) system	394:456	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	5	theme	electron	581:588	arg1	SEM					602:604	SEM	602:604	SEM	602:604	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	5	theme	electron	581:588	arg1	microscope					590:599	scanning electron microscope	572:599	scanning electron microscope (SEM)	572:605	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	6	theme	cerasifera	357:366	arg1	gum					373:375	Prunus cerasifera tree gum	350:375	Prunus cerasifera tree gum	350:375	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	7	7	theme	environmentally	1665:1679	arg1	materials					1699:1707	large-scale environmentally friendly advanced materials	1653:1707	large-scale environmentally friendly advanced materials with various potential applications	1653:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	2	8	theme	obtained	490:497	arg1	copolymer					505:513	the obtained PG-AM copolymer	486:513	the obtained PG-AM copolymer	486:513	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	0	9	theme	gum	99:101	arg1	polysaccharide					103:116	Prunus cerasifera gum polysaccharide	81:116	Prunus cerasifera gum polysaccharide	81:116	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	4	10	theme	potassium	1128:1136	arg1	50 °C					1162:1166	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	2	11	theme	scanning	572:579	arg1	SEM					602:604	SEM	602:604	SEM	602:604	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	11	theme	scanning	572:579	arg1	microscope					590:599	scanning electron microscope	572:599	scanning electron microscope (SEM)	572:605	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	5	12	theme	diameters	1280:1288	arg1	distribution					1290:1301	a diameters distribution	1278:1301	a diameters distribution between 2 and 10 μm	1278:1321	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	0	13	theme	cerasifera	88:97	arg1	polysaccharide					103:116	Prunus cerasifera gum polysaccharide	81:116	Prunus cerasifera gum polysaccharide	81:116	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	2	14	theme	PG-AM	499:503	arg1	copolymer					505:513	the obtained PG-AM copolymer	486:513	the obtained PG-AM copolymer	486:513	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	15	theme	Prunus	350:355	arg1	gum					373:375	Prunus cerasifera tree gum	350:375	Prunus cerasifera tree gum	350:375	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	1	16	with	copolymer	134:142	arg1	stability					167:175	excellent thermal stability	149:175	excellent thermal stability	149:175	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	16	with	copolymer	134:142	arg1	capacity					190:197	swelling capacity	181:197	swelling capacity	181:197	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	2	17	theme	thermogravimetric	608:624	arg1	TGA					636:638	TGA	636:638	TGA	636:638	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	17	theme	thermogravimetric	608:624	arg1	analysis					626:633	thermogravimetric analysis	608:633	thermogravimetric analysis (TGA)	608:639	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	4	18	theme	copolymerization	1042:1057	arg1	acrylamide					1103:1112	copolymerization between polysaccharide and 3 times (weight) acrylamide	1042:1112	copolymerization between polysaccharide and 3 times (weight) acrylamide	1042:1112	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	1	19	theme	swelling	181:188	arg1	capacity					190:197	swelling capacity	181:197	swelling capacity	181:197	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	0	20	theme	gum	46:48	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	0	20	theme	gum	46:48	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	2	21	theme	13C	473:475	arg1	NMR					477:479	13C NMR	473:479	13C NMR	473:479	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	22	theme	scanning	658:665	arg1	DSC					680:682	DSC	680:682	DSC	680:682	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	22	theme	scanning	658:665	arg1	calorimetry					667:677	differential scanning calorimetry	645:677	differential scanning calorimetry (DSC)	645:683	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	3	23	theme	tree	793:796	arg1	gum					798:800	Prunus cerasifera tree gum	775:800	Prunus cerasifera tree gum	775:800	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	5	24	theme	PG-AM	1256:1260	arg1	materials					1262:1270	the prepared PG-AM materials	1243:1270	the prepared PG-AM materials	1243:1270	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	2	25	dep	Fourier	536:542	arg1	transform					544:552	transform	544:552	transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively	544:697	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	6	26	theme	synthesized	1346:1356	arg1	PG-AM					1368:1372	the synthesized copolymer PG-AM	1342:1372	the synthesized copolymer PG-AM	1342:1372	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	2	27	theme	differential	645:656	arg1	DSC					680:682	DSC	680:682	DSC	680:682	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	27	theme	differential	645:656	arg1	calorimetry					667:677	differential scanning calorimetry	645:677	differential scanning calorimetry (DSC)	645:683	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	5	28	theme	conjoint	1211:1218	arg1	pores					1220:1224	isolated and conjoint pores	1198:1224	isolated and conjoint pores	1198:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	4	29	theme	grafting	955:962	arg1	efficiency					964:973	the grafting efficiency	951:973	the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM	951:1023	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	7	30	theme	various	1714:1720	arg1	applications					1732:1743	various potential applications	1714:1743	various potential applications	1714:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	4	31	theme	at	1159:1160	arg1	50 °C					1162:1166	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	5	32	theme	pores	1220:1224	arg1	pores					1220:1224	isolated and conjoint pores	1198:1224	isolated and conjoint pores	1198:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	5	32	theme	pores	1220:1224	arg1	lots					1190:1193	lots	1190:1193	lots of isolated and conjoint pores	1190:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	7	33	theme	synthesized	1597:1607	arg1	copolymer					1609:1617	the synthesized copolymer	1593:1617	the synthesized copolymer	1593:1617	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	4	34	theme	initiator	1149:1157	arg1	50 °C					1162:1166	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	2	35	theme	monosaccharide	301:314	arg1	compositions					316:327	The monosaccharide compositions	297:327	The monosaccharide compositions	297:327	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	6	36	theme	swelling	1431:1438	arg1	capacity					1440:1447	swelling capacity	1431:1447	swelling capacity	1431:1447	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	7	37	theme	excellent	1503:1511	arg1	stability					1521:1529	excellent thermal stability	1503:1529	excellent thermal stability	1503:1529	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	4	38	theme	3 mmol/L	1119:1126	arg1	50 °C					1162:1166	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	7	39	theme	detailed	1454:1461	arg1	characteristic					1474:1487	The detailed structural characteristic	1450:1487	The detailed structural characteristic together with excellent thermal stability and swelling properties	1450:1553	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	40	theme	friendly	1681:1688	arg1	materials					1699:1707	large-scale environmentally friendly advanced materials	1653:1707	large-scale environmentally friendly advanced materials with various potential applications	1653:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	41	theme	copolymer	1609:1617	arg1	precursor					1624:1632	a precursor	1622:1632	a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications	1622:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	41	theme	copolymer	1609:1617	arg1	utilization					1578:1588	efficient utilization	1568:1588	efficient utilization of the synthesized copolymer	1568:1617	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	2	42	theme	chromatography	428:441	arg1	system					451:456	a high-performance anion exchange chromatography (HPAEC) system	394:456	a high-performance anion exchange chromatography (HPAEC) system	394:456	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	0	43	theme	tree	41:44	arg1	gum					46:48	porous tree gum	34:48	porous tree gum	34:48	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	5	44	theme	prepared	1247:1254	arg1	materials					1262:1270	the prepared PG-AM materials	1243:1270	the prepared PG-AM materials	1243:1270	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	6	45	theme	copolymer	1358:1366	arg1	PG-AM					1368:1372	the synthesized copolymer PG-AM	1342:1372	the synthesized copolymer PG-AM	1342:1372	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	1	46	theme	soluble	226:232	arg1	polysaccharide					256:269	water soluble Prunus cerasifera gum polysaccharide	220:269	water soluble Prunus cerasifera gum polysaccharide (PG)	220:274	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	46	theme	soluble	226:232	arg1	PG					272:273	PG	272:273	PG	272:273	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	6	47	from	performance	1394:1404	arg1	stability					1417:1425	thermal stability	1409:1425	thermal stability	1409:1425	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	6	47	from	performance	1394:1404	arg1	capacity					1440:1447	swelling capacity	1431:1447	swelling capacity	1431:1447	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	3	48	theme	minor	877:881	arg1	mannose					901:907	mannose	901:907	mannose	901:907	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	48	theme	minor	877:881	arg1	acids					920:924	uronic acids	913:924	uronic acids	913:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	48	theme	minor	877:881	arg1	xylose					893:898	xylose	893:898	xylose	893:898	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	48	theme	minor	877:881	arg1	amount					883:888	minor amount	877:888	minor amount of xylose, mannose and uronic acids	877:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	2	49	theme	exchange	419:426	arg1	HPAEC					444:448	HPAEC	444:448	HPAEC	444:448	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	49	theme	exchange	419:426	arg1	chromatography					428:441	a high-performance anion exchange chromatography	394:441	a high-performance anion exchange chromatography (HPAEC) system	394:456	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	0	50	theme	porous	34:39	arg1	gum					46:48	porous tree gum	34:48	porous tree gum	34:48	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	7	51	dep	detailed	1454:1461	arg1	structural					1463:1472	structural	1463:1472	structural	1463:1472	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	4	52	theme	maximum	931:937	arg1	percent					939:945	The maximum percent	927:945	The maximum percent	927:945	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	7	53	theme	large-scale	1653:1663	arg1	materials					1699:1707	large-scale environmentally friendly advanced materials	1653:1707	large-scale environmentally friendly advanced materials with various potential applications	1653:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	3	54	theme	mannose	901:907	arg1	mannose					901:907	mannose	901:907	mannose	901:907	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	54	theme	mannose	901:907	arg1	acids					920:924	uronic acids	913:924	uronic acids	913:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	54	theme	mannose	901:907	arg1	xylose					893:898	xylose	893:898	xylose	893:898	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	54	theme	mannose	901:907	arg1	amount					883:888	minor amount	877:888	minor amount of xylose, mannose and uronic acids	877:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	2	55	theme	anion	413:417	arg1	HPAEC					444:448	HPAEC	444:448	HPAEC	444:448	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	55	theme	anion	413:417	arg1	chromatography					428:441	a high-performance anion exchange chromatography	394:441	a high-performance anion exchange chromatography (HPAEC) system	394:456	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	7	56	theme	efficient	1568:1576	arg1	precursor					1624:1632	a precursor	1622:1632	a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications	1622:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	56	theme	efficient	1568:1576	arg1	utilization					1578:1588	efficient utilization	1568:1588	efficient utilization of the synthesized copolymer	1568:1617	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	57	theme	swelling	1535:1542	arg1	properties					1544:1553	swelling properties	1535:1553	swelling properties	1535:1553	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	7	58	theme	potential	1722:1730	arg1	applications					1732:1743	various potential applications	1714:1743	various potential applications	1714:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	0	59	attach	derived	68:74	arg2	copolymer					58:66	copolymer	58:66	copolymer derived from Prunus cerasifera gum polysaccharide	58:116	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	0	59	attach	derived	68:74	arg1	polysaccharide					103:116	Prunus cerasifera gum polysaccharide	81:116	Prunus cerasifera gum polysaccharide	81:116	Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide.					
31004636	1	60	theme	Porous	119:124	arg1	copolymer					134:142	Porous grafted copolymer	119:142	Porous grafted copolymer with excellent thermal stability and swelling capacity	119:197	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	2	61	theme	1H	462:463	arg1	NMR					465:467	1H NMR	462:467	1H NMR	462:467	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	7	62	with	materials	1699:1707	arg1	applications					1732:1743	various potential applications	1714:1743	various potential applications	1714:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	6	63	theme	excellent	1384:1392	arg1	performance					1394:1404	an excellent performance	1381:1404	an excellent performance in thermal stability and swelling capacity	1381:1447	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	3	64	theme	xylose	893:898	arg1	mannose					901:907	mannose	901:907	mannose	901:907	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	64	theme	xylose	893:898	arg1	acids					920:924	uronic acids	913:924	uronic acids	913:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	64	theme	xylose	893:898	arg1	xylose					893:898	xylose	893:898	xylose	893:898	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	3	64	theme	xylose	893:898	arg1	amount					883:888	minor amount	877:888	minor amount of xylose, mannose and uronic acids	877:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	1	65	theme	grafted	126:132	arg1	copolymer					134:142	Porous grafted copolymer	119:142	Porous grafted copolymer with excellent thermal stability and swelling capacity	119:197	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	6	66	theme	thermal	1409:1415	arg1	stability					1417:1425	thermal stability	1409:1425	thermal stability	1409:1425	Compared with PG, the synthesized copolymer PG-AM showed an excellent performance in thermal stability and swelling capacity.					
31004636	3	67	theme	soluble	737:743	arg1	polysaccharides					745:759	the water soluble polysaccharides	727:759	the water soluble polysaccharides obtained from Prunus cerasifera tree gum	727:800	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	7	68	theme	thermal	1513:1519	arg1	stability					1521:1529	excellent thermal stability	1503:1529	excellent thermal stability	1503:1529	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	4	69	theme	persulfate	1138:1147	arg1	50 °C					1162:1166	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	7	70	theme	materials	1699:1707	arg1	preparation					1638:1648	preparation	1638:1648	preparation of large-scale environmentally friendly advanced materials with various potential applications	1638:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	5	71	theme	isolated	1198:1205	arg1	pores					1220:1224	isolated and conjoint pores	1198:1224	isolated and conjoint pores	1198:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	1	72	theme	cerasifera	241:250	arg1	polysaccharide					256:269	water soluble Prunus cerasifera gum polysaccharide	220:269	water soluble Prunus cerasifera gum polysaccharide (PG)	220:274	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	72	theme	cerasifera	241:250	arg1	PG					272:273	PG	272:273	PG	272:273	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	4	73	from	50 °C	1162:1166	arg1	initiator					1149:1157	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	4	73	from	50 °C	1162:1166	arg1	potassium					1128:1136	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	4	73	from	50 °C	1162:1166	arg1	at					1159:1160	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	4	73	from	50 °C	1162:1166	arg1	persulfate					1138:1147	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	4	73	from	50 °C	1162:1166	arg1	3 mmol/L					1119:1126	3 mmol/L potassium persulfate initiator at 50 °C	1119:1166	3 mmol/L potassium persulfate initiator at 50 °C for 1 h	1119:1174	The maximum percent and the grafting efficiency of grafting acrylamide (AM) onto PG to form PG-AM were obtained by copolymerization between polysaccharide and 3 times (weight) acrylamide with 3 mmol/L potassium persulfate initiator at 50 °C for 1 h.					
31004636	2	74	theme	gum	373:375	arg1	compositions					316:327	The monosaccharide compositions	297:327	The monosaccharide compositions	297:327	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	74	theme	gum	373:375	arg1	structure					337:345	the structure	333:345	the structure of Prunus cerasifera tree gum	333:375	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	1	75	theme	gum	252:254	arg1	polysaccharide					256:269	water soluble Prunus cerasifera gum polysaccharide	220:269	water soluble Prunus cerasifera gum polysaccharide (PG)	220:274	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	75	theme	gum	252:254	arg1	PG					272:273	PG	272:273	PG	272:273	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	76	theme	Prunus	234:239	arg1	polysaccharide					256:269	water soluble Prunus cerasifera gum polysaccharide	220:269	water soluble Prunus cerasifera gum polysaccharide (PG)	220:274	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	76	theme	Prunus	234:239	arg1	PG					272:273	PG	272:273	PG	272:273	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	1	77	theme	excellent	149:157	arg1	stability					167:175	excellent thermal stability	149:175	excellent thermal stability	149:175	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	5	78	located	observed	1231:1238	arg2	pores					1220:1224	isolated and conjoint pores	1198:1224	isolated and conjoint pores	1198:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	5	78	located	observed	1231:1238	arg2	lots					1190:1193	lots	1190:1193	lots of isolated and conjoint pores	1190:1224	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	5	78	located	observed	1231:1238	arg1	addition					1180:1187	addition	1180:1187	addition	1180:1187	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	5	78	located	observed	1231:1238	arg1	materials					1262:1270	the prepared PG-AM materials	1243:1270	the prepared PG-AM materials	1243:1270	In addition, lots of isolated and conjoint pores were observed in the prepared PG-AM materials, with a diameters distribution between 2 and 10 μm.					
31004636	2	79	dep	transform	544:552	arg1	infrared					554:561	infrared	554:561	infrared	554:561	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	2	80	dep	infrared	554:561	arg1	FT-IR					564:568	FT-IR	564:568	FT-IR	564:568	The monosaccharide compositions and the structure of Prunus cerasifera tree gum were detected by a high-performance anion exchange chromatography (HPAEC) system and 1H NMR and 13C NMR, and the obtained PG-AM copolymer was characterized by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively.					
31004636	3	81	theme	uronic	913:918	arg1	acids					920:924	uronic acids	913:924	uronic acids	913:924	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
31004636	1	82	theme	thermal	159:165	arg1	stability					167:175	excellent thermal stability	149:175	excellent thermal stability	149:175	Porous grafted copolymer with excellent thermal stability and swelling capacity was synthesized from water soluble Prunus cerasifera gum polysaccharide (PG) and acrylamide (AM).					
31004636	7	83	theme	advanced	1690:1697	arg1	materials					1699:1707	large-scale environmentally friendly advanced materials	1653:1707	large-scale environmentally friendly advanced materials with various potential applications	1653:1743	The detailed structural characteristic together with excellent thermal stability and swelling properties will benefit efficient utilization of the synthesized copolymer as a precursor for preparation of large-scale environmentally friendly advanced materials with various potential applications.					
31004636	3	84	theme	Prunus	775:780	arg1	gum					798:800	Prunus cerasifera tree gum	775:800	Prunus cerasifera tree gum	775:800	The results indicated that the water soluble polysaccharides obtained from Prunus cerasifera tree gum were mainly composed of l-arabinose (39.78%) and d-galactose (40.59%) with minor amount of xylose, mannose and uronic acids.					
30537500	0	0	theme	bone	90:93	arg1	engineering					102:112	bone tissue engineering	90:112	bone tissue engineering	90:112	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	2	1	theme	other	497:501	arg1	forms					511:515	other mineral forms	497:515	other mineral forms	497:515	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	2	theme	magnetic	609:616	arg1	composites					618:627	the magnetic composites	605:627	the magnetic composites	605:627	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	0	3	with	composites	33:42	arg1	inclusions					49:58	inclusions	49:58	inclusions of magnetic nanoparticles for bone tissue engineering	49:112	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	6	4	theme	calcium	1453:1459	arg1	Hya/Bsa					1483:1489	Hya/Bsa	1483:1489	Hya/Bsa	1483:1489	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	4	theme	calcium	1453:1459	arg1	chitosan					1473:1480	chitosan	1473:1480	chitosan	1473:1480	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	4	theme	calcium	1453:1459	arg1	phosphates					1461:1470	calcium phosphates	1453:1470	calcium phosphates	1453:1470	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	4	theme	calcium	1453:1459	arg1	MNPs					1511:1514	MNPs	1511:1514	MNPs	1511:1514	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	4	theme	calcium	1453:1459	arg1	%					1506:1506	more than 3%	1495:1506	more than 3% of MNPs	1495:1514	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	3	5	theme	scaffolds	921:929	arg1	formation					939:947	the formation	935:947	the formation of various types of calcium phosphates with amorphous nature	935:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	5	theme	scaffolds	921:929	arg1	composition					902:912	the composition	898:912	the composition of the scaffolds	898:929	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	6	with	phosphates	977:986	arg1	nature					1003:1008	amorphous nature	993:1008	amorphous nature	993:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	4	7	theme	in	1015:1016	arg1	studies					1036:1042	The in vitro degradation studies	1011:1042	The in vitro degradation studies	1011:1042	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	4	8	with	connection	1129:1138	arg1	phosphates					1177:1186	calcium phosphates	1169:1186	calcium phosphates	1169:1186	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	2	9	theme	biomimetic	342:351	arg1	strategy					353:360	The biomimetic strategy	338:360	The biomimetic strategy	338:360	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	6	10	theme	MNPs	1511:1514	arg1	Hya/Bsa					1483:1489	Hya/Bsa	1483:1489	Hya/Bsa	1483:1489	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	10	theme	MNPs	1511:1514	arg1	chitosan					1473:1480	chitosan	1473:1480	chitosan	1473:1480	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	10	theme	MNPs	1511:1514	arg1	phosphates					1461:1470	calcium phosphates	1453:1470	calcium phosphates	1453:1470	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	10	theme	MNPs	1511:1514	arg1	MNPs					1511:1514	MNPs	1511:1514	MNPs	1511:1514	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	6	10	theme	MNPs	1511:1514	arg1	%					1506:1506	more than 3%	1495:1506	more than 3% of MNPs	1495:1514	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	5	11	theme	osteoblasts	1333:1343	arg1	cell					1345:1348	osteoblasts cell	1333:1348	osteoblasts cell	1333:1348	The magnetic scaffolds exhibited no negative effect on osteoblasts cell, emphasizing a good biocompatibility.					
30537500	5	12	theme	negative	1314:1321	arg1	effect					1323:1328	no negative effect	1311:1328	no negative effect	1311:1328	The magnetic scaffolds exhibited no negative effect on osteoblasts cell, emphasizing a good biocompatibility.					
30537500	6	13	dep	in	1561:1562	arg1	vivo					1564:1567	vivo	1564:1567	vivo	1564:1567	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	4	14	theme	enzyme	1206:1211	arg1	access					1213:1218	the enzyme access	1202:1218	the enzyme access to the degradable components and material disintegration	1202:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	3	15	theme	Energy-dispersive	845:861	arg1	EDX					883:885	EDX	883:885	EDX	883:885	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	15	theme	Energy-dispersive	845:861	arg1	spectroscopy					869:880	Energy-dispersive X-ray spectroscopy	845:880	Energy-dispersive X-ray spectroscopy (EDX)	845:886	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	0	16	theme	tissue	95:100	arg1	engineering					102:112	bone tissue engineering	90:112	bone tissue engineering	90:112	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	2	17	theme	morphology	591:600	arg1	conditions					553:562	physiological conditions	539:562	physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids	539:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	18	theme	electron	653:660	arg1	microscopy					662:671	scanning electron microscopy	644:671	scanning electron microscopy (SEM)	644:677	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	18	theme	electron	653:660	arg1	SEM					674:676	SEM	674:676	SEM	674:676	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	6	19	theme	in	1561:1562	arg1	tests					1569:1573	in vivo tests	1561:1573	in vivo tests	1561:1573	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	3	20	theme	X-ray	863:867	arg1	EDX					883:885	EDX	883:885	EDX	883:885	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	20	theme	X-ray	863:867	arg1	spectroscopy					869:880	Energy-dispersive X-ray spectroscopy	845:880	Energy-dispersive X-ray spectroscopy (EDX)	845:886	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	2	21	theme	scanning	644:651	arg1	microscopy					662:671	scanning electron microscopy	644:671	scanning electron microscopy (SEM)	644:677	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	21	theme	scanning	644:651	arg1	SEM					674:676	SEM	674:676	SEM	674:676	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	22	theme	fluids	769:774	arg1	retention					735:743	the retention	731:743	the retention of simulated biological fluids	731:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	1	23	theme	hyaluronic	173:182	arg1	acid					184:187	hyaluronic acid	173:187	hyaluronic acid	173:187	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	23	theme	hyaluronic	173:182	arg1	biopolymers					150:160	biopolymers	150:160	biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin)	150:224	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	4	24	theme	material	1253:1260	arg1	disintegration					1262:1275	material disintegration	1253:1275	material disintegration	1253:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	2	25	theme	biological	758:767	arg1	fluids					769:774	simulated biological fluids	748:774	simulated biological fluids	748:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	26	theme	mineralization	385:398	arg1	processes					400:408	natural mineralization processes	377:408	natural mineralization processes	377:408	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	4	27	theme	degradation	1058:1068	arg1	process					1070:1076	a slow degradation process	1051:1076	a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration	1051:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	2	28	theme	simulated	748:756	arg1	fluids					769:774	simulated biological fluids	748:774	simulated biological fluids	748:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	29	theme	natural	377:383	arg1	processes					400:408	natural mineralization processes	377:408	natural mineralization processes	377:408	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	4	30	theme	slow	1053:1056	arg1	process					1070:1076	a slow degradation process	1051:1076	a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration	1051:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	1	31	theme	magnetic	255:262	arg1	nanoparticles					264:276	magnetic nanoparticles	255:276	magnetic nanoparticles	255:276	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	2	32	theme	composites	618:627	arg1	morphology					591:600	The morphology	587:600	The morphology of the magnetic composites	587:627	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	32	theme	composites	618:627	arg1	temperature					567:577	temperature	567:577	temperature	567:577	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	4	33	theme	polymeric	1147:1155	arg1	matrix					1157:1162	the polymeric matrix	1143:1162	the polymeric matrix	1143:1162	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	1	34	theme	bovine	193:198	arg1	albumin					206:212	bovine serum albumin	193:212	bovine serum albumin	193:212	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	34	theme	bovine	193:198	arg1	biopolymers					150:160	biopolymers	150:160	biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin)	150:224	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	0	35	theme	phosphates	22:31	arg1	composites					33:42	Calcium phosphates composites	14:42	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.	0:113	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	1	36	theme	nanoparticles	264:276	arg1	combination					135:145	combination	135:145	combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles	135:276	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	37	dep	biopolymers	150:160	arg1	albumin					206:212	bovine serum albumin	193:212	bovine serum albumin	193:212	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	37	dep	biopolymers	150:160	arg1	chitosan					163:170	chitosan	163:170	chitosan	163:170	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	37	dep	biopolymers	150:160	arg1	acid					184:187	hyaluronic acid	173:187	hyaluronic acid	173:187	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	37	dep	biopolymers	150:160	arg1	biopolymers					150:160	biopolymers	150:160	biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin)	150:224	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	3	38	theme	calcium	969:975	arg1	phosphates					977:986	calcium phosphates	969:986	calcium phosphates with amorphous nature	969:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	1	39	theme	serum	200:204	arg1	albumin					206:212	bovine serum albumin	193:212	bovine serum albumin	193:212	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	39	theme	serum	200:204	arg1	biopolymers					150:160	biopolymers	150:160	biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin)	150:224	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	0	40	theme	Calcium	14:20	arg1	composites					33:42	Calcium phosphates composites	14:42	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.	0:113	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	6	41	theme	scaffolds	1404:1412	arg1	properties					1414:1423	the scaffolds properties	1400:1423	the scaffolds properties	1400:1423	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	4	42	theme	matrix	1157:1162	arg1	connection					1129:1138	the tightly connection	1117:1138	the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration	1117:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	3	43	theme	various	952:958	arg1	types					960:964	various types	952:964	various types of calcium phosphates with amorphous nature	952:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	44	theme	transformed	785:795	arg1	spectroscopy					806:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	4	45	theme	calcium	1169:1175	arg1	phosphates					1177:1186	calcium phosphates	1169:1186	calcium phosphates	1169:1186	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	2	46	theme	physiological	539:551	arg1	conditions					553:562	physiological conditions	539:562	physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids	539:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	3	47	theme	types	960:964	arg1	formation					939:947	the formation	935:947	the formation of various types of calcium phosphates with amorphous nature	935:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	47	theme	types	960:964	arg1	composition					902:912	the composition	898:912	the composition of the scaffolds	898:929	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	2	48	dep	morphology	591:600	arg1	pH.					583:585	pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM)	583:677	pH.	583:585	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	3	49	theme	infrared	797:804	arg1	spectroscopy					806:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	4	50	theme	degradation	1024:1034	arg1	studies					1036:1042	The in vitro degradation studies	1011:1042	The in vitro degradation studies	1011:1042	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	3	51	theme	amorphous	993:1001	arg1	nature					1003:1008	amorphous nature	993:1008	amorphous nature	993:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	3	52	theme	X-ray	823:827	arg1	diffraction					829:839	X-ray diffraction	823:839	X-ray diffraction	823:839	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	2	53	theme	mineral	503:509	arg1	forms					511:515	other mineral forms	497:515	other mineral forms	497:515	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	4	54	theme	degradable	1227:1236	arg1	components					1238:1247	the degradable components	1223:1247	the degradable components	1223:1247	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	3	55	theme	Fourier	777:783	arg1	spectroscopy					806:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy	777:817	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	4	56	dep	in	1015:1016	arg1	vitro					1018:1022	vitro	1018:1022	vitro	1018:1022	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	3	57	theme	phosphates	977:986	arg1	types					960:964	various types	952:964	various types of calcium phosphates with amorphous nature	952:1008	Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature.					
30537500	4	58	theme	tightly	1121:1127	arg1	connection					1129:1138	the tightly connection	1117:1138	the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration	1117:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	1	59	theme	biomimetic	302:311	arg1	method					330:335	a biomimetic co-precipitation method	300:335	a biomimetic co-precipitation method	300:335	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	2	60	theme	macroporous	691:701	arg1	structure					703:711	a macroporous structure	689:711	a macroporous structure	689:711	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	2	61	theme	temperature	567:577	arg1	conditions					553:562	physiological conditions	539:562	physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids	539:774	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	4	62	theme	magnetic	1082:1089	arg1	composites					1091:1100	magnetic composites	1082:1100	magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration	1082:1275	The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration.					
30537500	5	63	theme	good	1365:1368	arg1	biocompatibility					1370:1385	a good biocompatibility	1363:1385	a good biocompatibility	1363:1385	The magnetic scaffolds exhibited no negative effect on osteoblasts cell, emphasizing a good biocompatibility.					
30537500	6	64	theme	further	1536:1542	arg1	optimization					1544:1555	further optimization	1536:1555	further optimization	1536:1555	Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.					
30537500	5	65	theme	magnetic	1282:1289	arg1	scaffolds					1291:1299	The magnetic scaffolds	1278:1299	The magnetic scaffolds	1278:1299	The magnetic scaffolds exhibited no negative effect on osteoblasts cell, emphasizing a good biocompatibility.					
30537500	0	66	theme	nanoparticles	72:84	arg1	inclusions					49:58	inclusions	49:58	inclusions of magnetic nanoparticles for bone tissue engineering	49:112	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	2	67	theme	synthesized	421:431	arg1	minerals					433:440	the synthesized minerals	417:440	the synthesized minerals	417:440	The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids.					
30537500	1	68	theme	biopolymers	150:160	arg1	combination					135:145	combination	135:145	combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles	135:276	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	69	theme	calcium	227:233	arg1	CP					247:248	CP	247:248	CP	247:248	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	1	69	theme	calcium	227:233	arg1	phosphates					235:244	calcium phosphates	227:244	calcium phosphates (CP)	227:249	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	0	70	theme	magnetic	63:70	arg1	nanoparticles					72:84	magnetic nanoparticles	63:84	magnetic nanoparticles for bone tissue engineering	63:112	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	1	71	theme	co-precipitation	313:328	arg1	method					330:335	a biomimetic co-precipitation method	300:335	a biomimetic co-precipitation method	300:335	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30537500	0	72	dep	Biopolymers	0:10	arg1	composites					33:42	Calcium phosphates composites	14:42	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.	0:113	Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.					
30537500	1	73	theme	phosphates	235:244	arg1	combination					135:145	combination	135:145	combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles	135:276	Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method.					
30770394	6	0	theme	control	916:922	arg1	population					924:933	the healthy control population	904:933	the healthy control population	904:933	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	8	1	with	patients	1105:1112	arg1	VWF					1123:1125	low VWF	1119:1125	low VWF	1119:1125	Importantly, we also observed evidence of aberrant glycosylation in a subgroup of patients with low VWF.					
30770394	2	2	theme	different	323:331	arg1	models					339:344	different mouse models	323:344	different mouse models	323:344	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	12	3	theme	qualitative	1723:1733	arg1	variations					1735:1744	quantitative and qualitative variations	1706:1744	quantitative and qualitative variations in VWF levels in the normal population	1706:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	10	4	theme	estimated	1332:1340	arg1	half-life					1346:1354	estimated VWF half-life	1332:1354	estimated VWF half-life	1332:1354	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	2	5	theme	von	279:281	arg1	VWD					303:305	VWD	303:305	VWD	303:305	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	2	5	theme	von	279:281	arg1	disease					294:300	von Willebrand disease	279:300	von Willebrand disease (VWD)	279:306	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	5	6	theme	healthy	792:798	arg1	controls					800:807	O blood group-matched healthy controls	770:807	O blood group-matched healthy controls	770:807	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	6	7	from	heterogeneity	853:865	arg1	expression					881:890	VWF glycan expression	870:890	VWF glycan expression	870:890	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	3	8	from	importance	471:480	arg1	pathogenesis					512:523	human VWD pathogenesis	502:523	human VWD pathogenesis	502:523	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	12	9	theme	quantitative	1706:1717	arg1	variations					1735:1744	quantitative and qualitative variations	1706:1744	quantitative and qualitative variations in VWF levels in the normal population	1706:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	4	10	theme	human	622:626	arg1	characterization					639:654	human VWF glycan characterization	622:654	human VWF glycan characterization	622:654	To address this, we developed a novel lectin-binding panel to enable human VWF glycan characterization.					
30770394	5	11	theme	glycan	697:702	arg1	expression					704:713	glycan expression	697:713	glycan expression	697:713	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	4	12	theme	glycan	632:637	arg1	characterization					639:654	human VWF glycan characterization	622:654	human VWF glycan characterization	622:654	To address this, we developed a novel lectin-binding panel to enable human VWF glycan characterization.					
30770394	9	13	theme	low	1207:1209	arg1	VWF					1211:1213	low VWF	1207:1213	low VWF	1207:1213	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	9	14	from	increase	1233:1240	arg1	exposure					1261:1268	galactose (Gal) exposure	1245:1268	galactose (Gal) exposure	1245:1268	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	7	15	theme	group	998:1002	arg1	expression					1004:1013	ABO(H) blood group expression	985:1013	ABO(H) blood group expression	985:1013	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	1	16	theme	von	121:123	arg1	VWF					144:146	VWF	144:146	VWF	144:146	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	1	16	theme	von	121:123	arg1	factor					136:141	von Willebrand factor	121:141	von Willebrand factor (VWF)	121:147	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	5	17	with	patients	734:741	arg1	VWF					752:754	low VWF	748:754	low VWF	748:754	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	10	18	theme	VWF	1401:1403	arg1	clearance					1405:1413	enhanced VWF clearance	1392:1413	enhanced VWF clearance	1392:1413	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	0	19	theme	von	75:77	arg1	factor					90:95	low von Willebrand factor	71:95	low von Willebrand factor	71:95	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30770394	9	20	theme	galactose	1245:1253	arg1	exposure					1261:1268	galactose (Gal) exposure	1245:1268	galactose (Gal) exposure	1245:1268	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	12	21	theme	carbohydrate	1654:1665	arg1	expression					1667:1676	VWF carbohydrate expression	1650:1676	VWF carbohydrate expression	1650:1676	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	11	22	theme	terminal	1477:1484	arg1	sialylation					1486:1496	terminal sialylation	1477:1496	terminal sialylation	1477:1496	Together, these findings support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinning low VWF in at least a subgroup of patients by promoting enhanced clearance.					
30770394	7	23	theme	terminal	960:967	arg1	sialylation					969:979	terminal sialylation	960:979	terminal sialylation	960:979	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	13	24	theme	#	1841:1841	arg1	NCT03167320					1842:1852	#NCT03167320	1841:1852	#NCT03167320	1841:1852	This trial was registered at www.clinicaltrials.gov as #NCT03167320.					
30770394	13	24	theme	#	1841:1841	arg1	trial					1791:1795	This trial	1786:1795	This trial	1786:1795	This trial was registered at www.clinicaltrials.gov as #NCT03167320.					
30770394	3	25	theme	accurate	420:427	arg1	assessment					429:438	accurate assessment	420:438	accurate assessment of VWF glycan composition	420:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	3	26	theme	VWD	508:510	arg1	pathogenesis					512:523	human VWD pathogenesis	502:523	human VWD pathogenesis	502:523	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	0	27	theme	low	71:73	arg1	factor					90:95	low von Willebrand factor	71:95	low von Willebrand factor	71:95	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30770394	0	28	theme	Increased	0:8	arg1	expression					20:29	Increased galactose expression	0:29	Increased galactose expression	0:29	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30770394	11	29	theme	sialylation	1486:1496	arg1	loss					1469:1472	loss	1469:1472	loss of terminal sialylation	1469:1496	Together, these findings support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinning low VWF in at least a subgroup of patients by promoting enhanced clearance.					
30770394	3	30	theme	glycan	447:452	arg1	composition					454:464	VWF glycan composition	443:464	VWF glycan composition	443:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	8	31	theme	aberrant	1065:1072	arg1	glycosylation					1074:1086	aberrant glycosylation	1065:1086	aberrant glycosylation	1065:1086	Importantly, we also observed evidence of aberrant glycosylation in a subgroup of patients with low VWF.					
30770394	6	32	theme	glycan	874:879	arg1	expression					881:890	VWF glycan expression	870:890	VWF glycan expression	870:890	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	3	33	theme	technical	383:391	arg1	challenges					393:402	the significant technical challenges	367:402	the significant technical challenges associated with accurate assessment of VWF glycan composition	367:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	7	34	dep	blood	992:996	arg1	H					989:989	H	989:989	H	989:989	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	7	34	dep	blood	992:996	arg1	ABO					985:987	ABO	985:987	ABO	985:987	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	5	35	theme	blood	772:776	arg1	controls					800:807	O blood group-matched healthy controls	770:807	O blood group-matched healthy controls	770:807	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	6	36	theme	interindividual	837:851	arg1	heterogeneity					853:865	significant interindividual heterogeneity	825:865	significant interindividual heterogeneity in VWF glycan expression	825:890	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	1	37	from	determinants	105:116	arg1	VWF					144:146	VWF	144:146	VWF	144:146	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	1	37	from	determinants	105:116	arg1	factor					136:141	von Willebrand factor	121:141	von Willebrand factor (VWF)	121:147	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	8	38	theme	patients	1105:1112	arg1	subgroup					1093:1100	a subgroup	1091:1100	a subgroup of patients with low VWF	1091:1125	Importantly, we also observed evidence of aberrant glycosylation in a subgroup of patients with low VWF.					
30770394	4	39	theme	novel	585:589	arg1	panel					606:610	a novel lectin-binding panel	583:610	a novel lectin-binding panel to enable human VWF glycan characterization	583:654	To address this, we developed a novel lectin-binding panel to enable human VWF glycan characterization.					
30770394	10	40	theme	Gal	1315:1317	arg1	exposure					1319:1326	Gal exposure	1315:1326	Gal exposure	1315:1326	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	3	41	theme	human	502:506	arg1	pathogenesis					512:523	human VWD pathogenesis	502:523	human VWD pathogenesis	502:523	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	8	42	theme	low	1119:1121	arg1	VWF					1123:1125	low VWF	1119:1125	low VWF	1119:1125	Importantly, we also observed evidence of aberrant glycosylation in a subgroup of patients with low VWF.					
30770394	12	43	theme	normal	1767:1772	arg1	population					1774:1783	the normal population	1763:1783	the normal population	1763:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	10	44	located	observed	1360:1367	arg1	patients					1378:1385	those patients	1372:1385	those patients with enhanced VWF clearance	1372:1413	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	10	44	located	observed	1360:1367	arg2	correlation					1295:1305	an inverse correlation	1284:1305	an inverse correlation between Gal exposure and estimated VWF half-life	1284:1354	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	5	45	theme	low	748:750	arg1	VWF					752:754	low VWF	748:754	low VWF	748:754	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	9	46	theme	α	1152:1152	arg1	sialylation					1166:1176	terminal α(2-6)-linked sialylation	1143:1176	terminal α(2-6)-linked sialylation	1143:1176	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	2	47	theme	mouse	333:337	arg1	models					339:344	different mouse models	323:344	different mouse models	323:344	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	6	48	theme	healthy	908:914	arg1	population					924:933	the healthy control population	904:933	the healthy control population	904:933	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	12	49	from	levels	1753:1758	arg1	population					1774:1783	the normal population	1763:1783	the normal population	1763:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	9	50	link	-linked	1158:1164	arg1	sialylation					1166:1176	terminal α(2-6)-linked sialylation	1143:1176	terminal α(2-6)-linked sialylation	1143:1176	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	10	51	theme	VWF	1342:1344	arg1	half-life					1346:1354	estimated VWF half-life	1332:1354	estimated VWF half-life	1332:1354	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	2	52	theme	Willebrand	283:292	arg1	VWD					303:305	VWD	303:305	VWD	303:305	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	2	52	theme	Willebrand	283:292	arg1	disease					294:300	von Willebrand disease	279:300	von Willebrand disease (VWD)	279:306	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	12	53	theme	VWF	1749:1751	arg1	levels					1753:1758	VWF levels	1749:1758	VWF levels in the normal population	1749:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	12	54	from	addition	1625:1632	arg1	likely					1682:1687	likely	1682:1687	likely	1682:1687	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	9	55	theme	-linked	1158:1164	arg1	sialylation					1166:1176	terminal α(2-6)-linked sialylation	1143:1176	terminal α(2-6)-linked sialylation	1143:1176	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	1	56	theme	critical	154:161	arg1	roles					163:167	critical roles	154:167	critical roles	154:167	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	11	57	theme	low	1546:1548	arg1	VWF					1550:1552	low VWF	1546:1552	low VWF	1546:1552	Together, these findings support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinning low VWF in at least a subgroup of patients by promoting enhanced clearance.					
30770394	5	58	used	used	683:686	arg2	methodology					662:672	This methodology	657:672	This methodology	657:672	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	3	59	theme	carbohydrates	485:497	arg1	importance					471:480	the importance	467:480	the importance of carbohydrates in human VWD pathogenesis	467:523	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	2	60	theme	Abnormal	232:239	arg1	glycosylation					241:253	Abnormal glycosylation	232:253	Abnormal glycosylation	232:253	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	9	61	gly	sialylation	1166:1176	arg1	patients					1193:1200	patients	1193:1200	patients with low VWF	1193:1213	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	9	61	gly	sialylation	1166:1176	arg1	particular					1131:1140	particular	1131:1140	particular	1131:1140	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	12	62	from	likely	1682:1687	arg1	addition					1625:1632	addition	1625:1632	addition	1625:1632	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	4	63	theme	VWF	628:630	arg1	characterization					639:654	human VWF glycan characterization	622:654	human VWF glycan characterization	622:654	To address this, we developed a novel lectin-binding panel to enable human VWF glycan characterization.					
30770394	1	64	theme	Willebrand	125:134	arg1	VWF					144:146	VWF	144:146	VWF	144:146	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	1	64	theme	Willebrand	125:134	arg1	factor					136:141	von Willebrand factor	121:141	von Willebrand factor (VWF)	121:147	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	5	65	theme	patients	734:741	arg1	cohort					720:725	a cohort	718:725	a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls	718:807	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	9	66	theme	secondary	1223:1231	arg1	increase					1233:1240	a secondary increase	1221:1240	a secondary increase in galactose (Gal) exposure	1221:1268	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	7	67	theme	blood	992:996	arg1	expression					1004:1013	ABO(H) blood group expression	985:1013	ABO(H) blood group expression	985:1013	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	12	68	from	alterations	1635:1645	arg1	expression					1667:1676	VWF carbohydrate expression	1650:1676	VWF carbohydrate expression	1650:1676	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	4	69	theme	lectin-binding	591:604	arg1	panel					606:610	a novel lectin-binding panel	583:610	a novel lectin-binding panel to enable human VWF glycan characterization	583:654	To address this, we developed a novel lectin-binding panel to enable human VWF glycan characterization.					
30770394	0	70	theme	Willebrand	79:88	arg1	factor					90:95	low von Willebrand factor	71:95	low von Willebrand factor	71:95	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30770394	10	71	theme	enhanced	1392:1399	arg1	clearance					1405:1413	enhanced VWF clearance	1392:1413	enhanced VWF clearance	1392:1413	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	12	72	theme	VWF	1650:1652	arg1	expression					1667:1676	VWF carbohydrate expression	1650:1676	VWF carbohydrate expression	1650:1676	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	10	73	with	patients	1378:1385	arg1	clearance					1405:1413	enhanced VWF clearance	1392:1413	enhanced VWF clearance	1392:1413	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	5	74	theme	group-matched	778:790	arg1	controls					800:807	O blood group-matched healthy controls	770:807	O blood group-matched healthy controls	770:807	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	12	75	from	variations	1735:1744	arg1	levels					1753:1758	VWF levels	1749:1758	VWF levels in the normal population	1749:1783	In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population.					
30770394	8	76	theme	glycosylation	1074:1086	arg1	evidence					1053:1060	evidence	1053:1060	evidence of aberrant glycosylation	1053:1086	Importantly, we also observed evidence of aberrant glycosylation in a subgroup of patients with low VWF.					
30770394	1	77	theme	Glycan	98:103	arg1	determinants					105:116	Glycan determinants	98:116	Glycan determinants on von Willebrand factor (VWF)	98:147	Glycan determinants on von Willebrand factor (VWF) play critical roles in regulating its susceptibility to proteolysis and clearance.					
30770394	11	78	theme	patients	1580:1587	arg1	subgroup					1568:1575	at least a subgroup	1557:1575	at least a subgroup of patients	1557:1587	Together, these findings support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinning low VWF in at least a subgroup of patients by promoting enhanced clearance.					
30770394	9	79	with	patients	1193:1200	arg1	VWF					1211:1213	low VWF	1207:1213	low VWF	1207:1213	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	3	80	theme	composition	454:464	arg1	assessment					429:438	accurate assessment	420:438	accurate assessment of VWF glycan composition	420:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	6	81	theme	VWF	870:872	arg1	expression					881:890	VWF glycan expression	870:890	VWF glycan expression	870:890	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	10	82	theme	inverse	1287:1293	arg1	correlation					1295:1305	an inverse correlation	1284:1305	an inverse correlation between Gal exposure and estimated VWF half-life	1284:1354	Furthermore, an inverse correlation between Gal exposure and estimated VWF half-life was observed in those patients with enhanced VWF clearance.					
30770394	3	83	theme	VWF	443:445	arg1	composition					454:464	VWF glycan composition	443:464	VWF glycan composition	443:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	5	84	theme	O	770:770	arg1	controls					800:807	O blood group-matched healthy controls	770:807	O blood group-matched healthy controls	770:807	This methodology was then used to study glycan expression in a cohort of 110 patients with low VWF compared with O blood group-matched healthy controls.					
30770394	3	85	theme	significant	371:381	arg1	challenges					393:402	the significant technical challenges	367:402	the significant technical challenges associated with accurate assessment of VWF glycan composition	367:464	However, because of the significant technical challenges associated with accurate assessment of VWF glycan composition, the importance of carbohydrates in human VWD pathogenesis remains largely unexplored.					
30770394	6	86	theme	significant	825:835	arg1	heterogeneity					853:865	significant interindividual heterogeneity	825:865	significant interindividual heterogeneity in VWF glycan expression	825:890	Interestingly, significant interindividual heterogeneity in VWF glycan expression was seen in the healthy control population.					
30770394	7	87	gly	included	951:958	arg1	VWF					1018:1020	VWF	1018:1020	VWF	1018:1020	This variation included terminal sialylation and ABO(H) blood group expression on VWF.					
30770394	0	88	with	patients	57:64	arg1	factor					90:95	low von Willebrand factor	71:95	low von Willebrand factor	71:95	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30770394	2	89	theme	models	339:344	arg1	number					313:318	a number	311:318	a number of different mouse models	311:344	Abnormal glycosylation has been shown to cause von Willebrand disease (VWD) in a number of different mouse models.					
30770394	11	90	theme	enhanced	1602:1609	arg1	clearance					1611:1619	enhanced clearance	1602:1619	enhanced clearance	1602:1619	Together, these findings support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinning low VWF in at least a subgroup of patients by promoting enhanced clearance.					
30770394	9	91	theme	terminal	1143:1150	arg1	sialylation					1166:1176	terminal α(2-6)-linked sialylation	1143:1176	terminal α(2-6)-linked sialylation	1143:1176	In particular, terminal α(2-6)-linked sialylation was reduced in patients with low VWF, with a secondary increase in galactose (Gal) exposure.					
30770394	0	92	theme	galactose	10:18	arg1	expression					20:29	Increased galactose expression	0:29	Increased galactose expression	0:29	Increased galactose expression and enhanced clearance in patients with low von Willebrand factor.					
30530886	0	0	theme	structures	84:93	arg1	synthesis					35:43	the synthesis	31:43	the synthesis of human milk oligosaccharide backbone structures	31:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	2	1	theme	purified	532:539	arg1	compound					541:548	a purified compound	530:548	a purified compound	530:548	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	2	1	theme	purified	532:539	arg1	oxazoline					419:427	the oxazoline	415:427	the oxazoline	415:427	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	1	2	theme	reactive	163:170	arg1	oxazoline					172:180	a reactive oxazoline	161:180	a reactive oxazoline	161:180	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	0	3	theme	backbone	75:82	arg1	structures					84:93	human milk oligosaccharide backbone structures	48:93	human milk oligosaccharide backbone structures	48:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	2	4	used	used	522:525	arg2	oxazoline					419:427	the oxazoline	415:427	the oxazoline	415:427	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	2	4	used	used	522:525	arg2	either					440:445	either	440:445	either	440:445	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	2	4	used	used	522:525	arg2	compound					541:548	a purified compound	530:548	a purified compound	530:548	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	5	theme	oxazoline	701:709	arg1	use					685:687	the use	681:687	the use of purified oxazoline	681:709	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	6	theme	hydrolase	1039:1047	arg1	family					1049:1054	the glycoside hydrolase family 20	1025:1057	the glycoside hydrolase family 20	1025:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	7	theme	highest	555:561	arg1	mM					656:657	13.7 mM	651:657	13.7 mM	651:657	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	7	theme	highest	555:561	arg1	concentration					572:584	The highest observed concentration	551:584	The highest observed concentration of overall transglycosylation products in a cascade reaction	551:645	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	2	8	theme	oligosaccharide	321:335	arg1	lacto-N-triose					347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers	306:386	lacto-N-triose	347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	9	theme	significant	914:924	arg1	reagents					937:944	reagents	937:944	reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20	937:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	9	theme	significant	914:924	arg1	triethylamine					954:966	triethylamine	954:966	triethylamine	954:966	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	9	theme	significant	914:924	arg1	amounts					926:932	significant amounts	914:932	significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20	914:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	10	theme	products	616:623	arg1	mM					656:657	13.7 mM	651:657	13.7 mM	651:657	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	10	theme	products	616:623	arg1	concentration					572:584	The highest observed concentration	551:584	The highest observed concentration of overall transglycosylation products in a cascade reaction	551:645	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	11	from	family	1049:1054	arg1	enzyme					1013:1018	an enzyme	1010:1018	an enzyme from the glycoside hydrolase family 20	1010:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	2	12	theme	milk	316:319	arg1	lacto-N-triose					347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers	306:386	lacto-N-triose	347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	13	theme	glycoside	1029:1037	arg1	family					1049:1054	the glycoside hydrolase family 20	1025:1057	the glycoside hydrolase family 20	1025:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	2	14	theme	human	310:314	arg1	lacto-N-triose					347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers	306:386	lacto-N-triose	347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	15	theme	overall	589:595	arg1	products					616:623	overall transglycosylation products	589:623	overall transglycosylation products	589:623	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	16	theme	reagents	937:944	arg1	reagents					937:944	reagents	937:944	reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20	937:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	16	theme	reagents	937:944	arg1	triethylamine					954:966	triethylamine	954:966	triethylamine	954:966	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	16	theme	reagents	937:944	arg1	amounts					926:932	significant amounts	914:932	significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20	914:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	17	theme	donor	185:189	arg1	molecule					191:198	donor molecule	185:198	donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures	185:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	0	18	theme	chemo-enzymatic	2:16	arg1	approach					18:25	A chemo-enzymatic approach	0:25	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures	0:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	3	19	theme	cascade	630:636	arg1	reaction					638:645	a cascade reaction	628:645	a cascade reaction	628:645	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	20	dep	mM	656:657	arg1	used					828:831	used	828:831	could be used without any further purification	819:864	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	2	21	theme	sequential	496:505	arg1	cascade					507:513	a chemo-enzymatic sequential cascade	478:513	a chemo-enzymatic sequential cascade	478:513	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	22	theme	in	791:792	arg1	oxazoline					809:817	the in situ-generated oxazoline	787:817	the in situ-generated oxazoline	787:817	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	2	23	theme	chemo-enzymatic	480:494	arg1	cascade					507:513	a chemo-enzymatic sequential cascade	478:513	a chemo-enzymatic sequential cascade	478:513	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	24	theme	situ-generated	794:807	arg1	oxazoline					809:817	the in situ-generated oxazoline	787:817	the in situ-generated oxazoline	787:817	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	25	from	concentration	572:584	arg1	reaction					638:645	a cascade reaction	628:645	a cascade reaction	628:645	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	26	theme	transglycosylation	204:221	arg1	reaction					223:230	transglycosylation reaction	204:230	transglycosylation reaction to synthesize human milk oligosaccharide backbone structures	204:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	2	27	theme	precursor	337:345	arg1	lacto-N-triose					347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers	306:386	lacto-N-triose	347:360	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	28	theme	purified	692:699	arg1	oxazoline					701:709	purified oxazoline	692:709	purified oxazoline	692:709	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	29	theme	first	995:999	arg1	time					1001:1004	the first time	991:1004	the first time for an enzyme from the glycoside hydrolase family 20	991:1057	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	0	30	theme	human	48:52	arg1	structures					84:93	human milk oligosaccharide backbone structures	48:93	human milk oligosaccharide backbone structures	48:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	1	31	theme	engineered	114:123	arg1	β-N-acetylhexosaminidase					125:148	an engineered β-N-acetylhexosaminidase	111:148	an engineered β-N-acetylhexosaminidase	111:148	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	3	32	theme	used	892:895	arg1	enzyme					897:902	the used enzyme	888:902	the used enzyme	888:902	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	33	theme	β-N-acetylhexosaminidase	125:148	arg1	ability					100:106	The ability	96:106	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures	96:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	1	34	theme	human	246:250	arg1	structures					282:291	human milk oligosaccharide backbone structures	246:291	human milk oligosaccharide backbone structures	246:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	3	35	used	used	828:831	arg2	oxazoline					809:817	the in situ-generated oxazoline	787:817	the in situ-generated oxazoline	787:817	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	36	theme	transglycosylation	597:614	arg1	products					616:623	overall transglycosylation products	589:623	overall transglycosylation products	589:623	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	37	theme	milk	252:255	arg1	structures					282:291	human milk oligosaccharide backbone structures	246:291	human milk oligosaccharide backbone structures	246:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	0	38	theme	oligosaccharide	59:73	arg1	structures					84:93	human milk oligosaccharide backbone structures	48:93	human milk oligosaccharide backbone structures	48:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	2	39	dep	either	440:445	arg1	situ-generated					450:463	situ-generated	450:463	situ-generated	450:463	The human milk oligosaccharide precursor lacto-N-triose II and three regioisomers could be synthesized using the oxazoline, which was either in situ-generated resulting in a chemo-enzymatic sequential cascade or was used as a purified compound.					
30530886	3	40	theme	observed	563:570	arg1	mM					656:657	13.7 mM	651:657	13.7 mM	651:657	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	40	theme	observed	563:570	arg1	concentration					572:584	The highest observed concentration	551:584	The highest observed concentration of overall transglycosylation products in a cascade reaction	551:645	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	41	theme	oligosaccharide	257:271	arg1	structures					282:291	human milk oligosaccharide backbone structures	246:291	human milk oligosaccharide backbone structures	246:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	0	42	theme	milk	54:57	arg1	structures					84:93	human milk oligosaccharide backbone structures	48:93	human milk oligosaccharide backbone structures	48:93	A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures.					
30530886	3	43	theme	transglycosylation	734:751	arg1	products					753:760	transglycosylation products	734:760	transglycosylation products	734:760	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	44	dep	reaction	223:230	arg1	synthesize					235:244	synthesize	235:244	to synthesize human milk oligosaccharide backbone structures	232:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
30530886	3	45	theme	products	753:760	arg1	mM					728:729	25.0 mM	723:729	25.0 mM of transglycosylation products after 6.5 h.	723:773	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	3	46	theme	further	845:851	arg1	purification					853:864	any further purification	841:864	any further purification	841:864	The highest observed concentration of overall transglycosylation products in a cascade reaction was 13.7 mM after 18.5 h, whereas the use of purified oxazoline resulted in 25.0 mM of transglycosylation products after 6.5 h. Remarkably, the in situ-generated oxazoline could be used without any further purification and it was shown that the used enzyme tolerated significant amounts of reagents such as triethylamine, which is reported for the first time for an enzyme from the glycoside hydrolase family 20.					
30530886	1	47	theme	backbone	273:280	arg1	structures					282:291	human milk oligosaccharide backbone structures	246:291	human milk oligosaccharide backbone structures	246:291	The ability of an engineered β-N-acetylhexosaminidase to utilize a reactive oxazoline as donor molecule for transglycosylation reaction to synthesize human milk oligosaccharide backbone structures was studied.					
31703890	5	0	theme	triple	789:794	arg1	detection					796:804	triple detection	789:804	triple detection	789:804	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	7	1	theme	structural	1348:1357	arg1	diversity					1359:1367	considerable structural diversity	1335:1367	considerable structural diversity	1335:1367	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	7	2	theme	considerable	1335:1346	arg1	diversity					1359:1367	considerable structural diversity	1335:1367	considerable structural diversity	1335:1367	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	1	3	theme	fucose-containing	172:188	arg1	polysaccharides					199:213	fucose-containing sulfated polysaccharides	172:213	fucose-containing sulfated polysaccharides	172:213	Fucoidans represent an intriguing class of fucose-containing sulfated polysaccharides.					
31703890	6	4	from	characteristics	1222:1236	arg1	solution					1294:1301	solution	1294:1301	solution	1294:1301	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	0	5	theme	size-exclusion	74:87	arg1	chromatography					89:102	size-exclusion chromatography	74:102	size-exclusion chromatography with multiple detection	74:126	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	8	6	dep	Fuc-FE	1528:1533	arg1	ones					1560:1563	the narrowest ones	1546:1563	the narrowest ones	1546:1563	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	0	7	with	chromatography	89:102	arg1	detection					118:126	multiple detection	109:126	multiple detection	109:126	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	1	8	theme	sulfated	190:197	arg1	polysaccharides					199:213	fucose-containing sulfated polysaccharides	172:213	fucose-containing sulfated polysaccharides	172:213	Fucoidans represent an intriguing class of fucose-containing sulfated polysaccharides.					
31703890	10	9	theme	side	1749:1752	arg1	chains					1754:1759	partly long side chains	1737:1759	partly long side chains	1737:1759	The conformation data suggest branched structures with partly long side chains.					
31703890	0	10	theme	multiple	109:116	arg1	detection					118:126	multiple detection	109:126	multiple detection	109:126	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	7	11	theme	large	1379:1383	arg1	differences					1385:1395	large differences	1379:1395	large differences in their MW profiles	1379:1416	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	7	12	theme	tested	1308:1313	arg1	fucoidans					1315:1323	The tested fucoidans	1304:1323	The tested fucoidans	1304:1323	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	5	13	theme	column	760:765	arg1	calibration					767:777	column calibration	760:777	column calibration	760:777	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	6	14	theme	present	918:924	arg1	study					926:930	the present study	914:930	the present study	914:930	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	5	15	dep	Mw	837:838	arg1	only					819:822	only	819:822	only	819:822	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	3	16	theme	whole	579:583	arg1	molecules					585:593	the whole molecules	575:593	the whole molecules	575:593	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	9	17	theme	expanded	1640:1647	arg1	chains					1658:1663	expanded flexible chains	1640:1663	expanded flexible chains in PBS solution	1640:1679	Most of the fucoidan fractions (except for Fuc-DF) turned out to exist as expanded flexible chains in PBS solution.					
31703890	6	18	from	conformation	1278:1289	arg1	solution					1294:1301	solution	1294:1301	solution	1294:1301	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	3	19	theme	glycan	532:537	arg1	structure					539:547	the glycan structure	528:547	the glycan structure	528:547	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	9	20	theme	flexible	1649:1656	arg1	chains					1658:1663	expanded flexible chains	1640:1663	expanded flexible chains in PBS solution	1640:1679	Most of the fucoidan fractions (except for Fuc-DF) turned out to exist as expanded flexible chains in PBS solution.					
31703890	11	21	theme	further	1813:1819	arg1	fractionation					1821:1833	further fractionation	1813:1833	further fractionation	1813:1833	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	2	22	theme	chain	414:418	arg1	conformation					420:431	chain conformation	414:431	chain conformation	414:431	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	1	23	theme	polysaccharides	199:213	arg1	class					163:167	an intriguing class	149:167	an intriguing class of fucose-containing sulfated polysaccharides	149:213	Fucoidans represent an intriguing class of fucose-containing sulfated polysaccharides.					
31703890	6	24	used	used	936:939	arg2	we					933:934	we	933:934	we	933:934	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	25	from	distribution	1242:1253	arg1	solution					1294:1301	solution	1294:1301	solution	1294:1301	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	11	26	theme	various	1940:1946	arg1	fucoidans					1948:1956	the various fucoidans	1936:1956	the various fucoidans	1936:1956	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	3	27	theme	spectrometry	475:486	arg1	techniques					488:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	5	28	theme	absolute	828:835	arg1	Mw					837:838	the absolute Mw	824:838	the absolute Mw	824:838	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	6	29	dep	F.	1017:1018	arg1	serratus					1020:1027	F. serratus (FS)	1017:1032	F. serratus (FS)	1017:1032	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	30	theme	Mw	1172:1173	arg1	mass					1166:1169	their molar mass	1154:1169	their molar mass (Mw, Mn, Mp, dispersity)	1154:1194	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	30	theme	Mw	1172:1173	arg1	dispersity					1184:1193	Mw, Mn, Mp, dispersity	1172:1193	dispersity	1184:1193	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	11	31	theme	structural	1839:1848	arg1	characterization					1850:1865	structural characterization	1839:1865	structural characterization	1839:1865	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	3	32	theme	NMR	462:464	arg1	techniques					488:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	0	33	theme	Size	0:3	arg1	distribution					5:16	Size distribution	0:16	Size distribution	0:16	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	9	34	theme	fucoidan	1578:1585	arg1	fractions					1587:1595	the fucoidan fractions	1574:1595	the fucoidan fractions	1574:1595	Most of the fucoidan fractions (except for Fuc-DF) turned out to exist as expanded flexible chains in PBS solution.					
31703890	11	35	theme	bioactivity	1904:1914	arg1	differences					1916:1926	the bioactivity differences	1900:1926	the bioactivity differences between the various fucoidans	1900:1956	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	3	36	theme	native	604:609	arg1	state					611:615	their native state	598:615	their native state	598:615	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	11	37	theme	differences	1916:1926	arg1	fractionation					1821:1833	further fractionation	1813:1833	further fractionation	1813:1833	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	11	37	theme	differences	1916:1926	arg1	characterization					1850:1865	structural characterization	1839:1865	structural characterization	1839:1865	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	11	37	theme	differences	1916:1926	arg1	interpretation					1882:1895	the interpretation	1878:1895	the interpretation of the bioactivity differences between the various fucoidans	1878:1956	The knowledge obtained by this study is useful for further fractionation and structural characterization as well as the interpretation of the bioactivity differences between the various fucoidans.					
31703890	0	38	theme	chain	22:26	arg1	conformation					28:39	chain conformation	22:39	chain conformation	22:39	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	5	39	theme	MW	731:732	arg1	determination					734:746	traditional MW determination	719:746	traditional MW determination by SEC with column calibration	719:777	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	10	40	with	structures	1721:1730	arg1	chains					1754:1759	partly long side chains	1737:1759	partly long side chains	1737:1759	The conformation data suggest branched structures with partly long side chains.					
31703890	2	41	theme	sulfation	353:361	arg1	conformation					420:431	chain conformation	414:431	chain conformation	414:431	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	2	41	theme	sulfation	353:361	arg1	degree					343:348	their degree	337:348	their degree of sulfation	337:361	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	2	41	theme	sulfation	353:361	arg1	distribution					397:408	molecular weight (MW) distribution	375:408	molecular weight (MW) distribution	375:408	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	10	42	theme	conformation	1686:1697	arg1	data					1699:1702	The conformation data	1682:1702	The conformation data	1682:1702	The conformation data suggest branched structures with partly long side chains.					
31703890	6	43	dep	F.	1035:1036	arg1	evanescens					1038:1047	F. evanescens (FE)	1035:1052	F. evanescens (FE)	1035:1052	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	2	44	theme	biological	220:229	arg1	related					271:277	related	271:277	related	271:277	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	2	44	theme	biological	220:229	arg1	activities					231:240	The biological activities	216:240	The biological activities of these polysaccharides	216:265	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	5	45	with	SEC	751:753	arg1	calibration					767:777	column calibration	760:777	column calibration	760:777	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	6	46	dep	radius	1210:1215	arg1	Rh					1218:1219	Rh	1218:1219	Rh	1218:1219	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	3	47	theme	structure	539:547	arg1	details					517:523	details	517:523	details of the glycan structure	517:547	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	10	48	theme	long	1744:1747	arg1	chains					1754:1759	partly long side chains	1737:1759	partly long side chains	1737:1759	The conformation data suggest branched structures with partly long side chains.					
31703890	6	49	theme	mass	1166:1169	arg1	characteristics					1222:1236	their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics	1154:1236	their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics	1154:1236	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	4	50	theme	fucoidans	669:677	arg1	latter					655:660	latter	655:660	latter	655:660	Accordingly, the knowledge about the latter of the fucoidans is currently still limited.					
31703890	3	51	theme	mass	470:473	arg1	spectrometry					475:486	mass spectrometry	470:486	mass spectrometry	470:486	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	3	52	theme	Modern	455:460	arg1	techniques					488:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques	455:497	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	6	53	theme	molar	1160:1164	arg1	dispersity					1184:1193	Mw, Mn, Mp, dispersity	1172:1193	dispersity	1184:1193	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	53	theme	molar	1160:1164	arg1	mass					1166:1169	their molar mass	1154:1169	their molar mass (Mw, Mn, Mp, dispersity)	1154:1194	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	2	54	theme	important	438:446	arg1	roles					448:452	important roles	438:452	important roles	438:452	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	0	55	theme	different	48:56	arg1	fucoidans					58:66	six different fucoidans	44:66	six different fucoidans	44:66	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	5	56	theme	molecule	885:892	arg1	characteristics					894:908	additional molecule characteristics	874:908	additional molecule characteristics	874:908	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	9	57	from	chains	1658:1663	arg1	solution					1672:1679	PBS solution	1668:1679	PBS solution	1668:1679	Most of the fucoidan fractions (except for Fuc-DF) turned out to exist as expanded flexible chains in PBS solution.					
31703890	5	58	from	information	859:869	arg1	characteristics					894:908	additional molecule characteristics	874:908	additional molecule characteristics	874:908	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	2	59	theme	weight	385:390	arg1	distribution					397:408	molecular weight (MW) distribution	375:408	molecular weight (MW) distribution	375:408	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	6	60	theme	rms	1206:1208	arg1	size					1200:1203	size	1200:1203	size (rms radius, Rh)	1200:1220	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	60	theme	rms	1206:1208	arg1	radius					1210:1215	rms radius	1206:1215	rms radius	1206:1215	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	8	61	theme	broadest	1490:1497	arg1	those					1517:1521	those	1517:1521	those	1517:1521	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	8	61	theme	broadest	1490:1497	arg1	distributions					1502:1514	the broadest MW distributions	1486:1514	the broadest MW distributions	1486:1514	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	6	62	theme	size	1200:1203	arg1	characteristics					1222:1236	their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics	1154:1236	their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics	1154:1236	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	2	63	theme	polysaccharides	251:265	arg1	related					271:277	related	271:277	related	271:277	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	2	63	theme	polysaccharides	251:265	arg1	activities					231:240	The biological activities	216:240	The biological activities of these polysaccharides	216:265	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	6	64	dep	Dictyosiphon	1055:1066	arg1	foeniculaceus					1068:1080	Dictyosiphon foeniculaceus (DF)	1055:1085	Dictyosiphon foeniculaceus (DF)	1055:1085	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	9	65	theme	PBS	1668:1670	arg1	solution					1672:1679	PBS solution	1668:1679	PBS solution	1668:1679	Most of the fucoidan fractions (except for Fuc-DF) turned out to exist as expanded flexible chains in PBS solution.					
31703890	2	66	theme	molecular	375:383	arg1	MW					393:394	MW	393:394	MW	393:394	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	2	66	theme	molecular	375:383	arg1	weight					385:390	molecular weight	375:390	molecular weight (MW) distribution	375:408	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	8	67	theme	narrowest	1550:1558	arg1	ones					1560:1563	the narrowest ones	1546:1563	the narrowest ones	1546:1563	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	3	68	theme	molecules	585:593	arg1	structure					562:570	the structure	558:570	the structure of the whole molecules	558:593	Modern NMR and mass spectrometry techniques allow elucidating details of the glycan structure, but not the structure of the whole molecules in their native state.					
31703890	5	69	theme	traditional	719:729	arg1	determination					734:746	traditional MW determination	719:746	traditional MW determination by SEC with column calibration	719:777	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	6	70	dep	Laminaria	1088:1096	arg1	digitata					1098:1105	Laminaria digitata (LD)	1088:1110	Laminaria digitata (LD)	1088:1110	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	2	71	theme	structural	306:315	arg1	parameters					317:326	their compositional and structural parameters	282:326	their compositional and structural parameters	282:326	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	6	72	dep	dispersity	1184:1193	arg1	Mp					1180:1181	Mw, Mn, Mp, dispersity	1172:1193	Mp	1180:1181	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	6	72	dep	dispersity	1184:1193	arg1	Mn					1176:1177	Mw, Mn, Mp, dispersity	1172:1193	Mn	1176:1177	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	1	73	theme	intriguing	152:161	arg1	class					163:167	an intriguing class	149:167	an intriguing class of fucose-containing sulfated polysaccharides	149:213	Fucoidans represent an intriguing class of fucose-containing sulfated polysaccharides.					
31703890	8	74	theme	MW	1499:1500	arg1	those					1517:1521	those	1517:1521	those	1517:1521	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	8	74	theme	MW	1499:1500	arg1	distributions					1502:1514	the broadest MW distributions	1486:1514	the broadest MW distributions	1486:1514	Fuc-FV and Fuc-SL showed the broadest MW distributions, those from Fuc-FE and Fuc-DF the narrowest ones.					
31703890	7	75	theme	MW	1406:1407	arg1	profiles					1409:1416	their MW profiles	1400:1416	their MW profiles	1400:1416	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	5	76	with	SEC	780:782	arg1	detection					796:804	triple detection	789:804	triple detection	789:804	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	6	77	theme	chain	1272:1276	arg1	conformation					1278:1289	their chain conformation	1266:1289	their chain conformation in solution	1266:1301	In the present study, we used this method to compare six fucoidans extracted from Fucus vesiculosus (FV), F. serratus (FS), F. evanescens (FE), Dictyosiphon foeniculaceus (DF), Laminaria digitata (LD), and Saccharina latissima (SL) concerning their molar mass (Mw, Mn, Mp, dispersity) and size (rms radius, Rh) characteristics and distribution as well as their chain conformation in solution.					
31703890	5	78	theme	additional	874:883	arg1	characteristics					894:908	additional molecule characteristics	874:908	additional molecule characteristics	874:908	Contrary to traditional MW determination by SEC with column calibration, SEC with triple detection provides not only the absolute Mw, but can also give information on additional molecule characteristics.					
31703890	0	79	theme	fucoidans	58:66	arg1	distribution					5:16	Size distribution	0:16	Size distribution	0:16	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	0	79	theme	fucoidans	58:66	arg1	conformation					28:39	chain conformation	22:39	chain conformation	22:39	Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection.					
31703890	7	80	from	differences	1385:1395	arg1	profiles					1409:1416	their MW profiles	1400:1416	their MW profiles	1400:1416	The tested fucoidans displayed considerable structural diversity including large differences in their MW profiles and showed to be heterogeneously composed.					
31703890	2	81	theme	compositional	288:300	arg1	parameters					317:326	their compositional and structural parameters	282:326	their compositional and structural parameters	282:326	The biological activities of these polysaccharides are related to their compositional and structural parameters, whereby their degree of sulfation, as well as molecular weight (MW) distribution and chain conformation play important roles.					
31703890	10	82	theme	branched	1712:1719	arg1	structures					1721:1730	branched structures	1712:1730	branched structures with partly long side chains	1712:1759	The conformation data suggest branched structures with partly long side chains.					
31174247	0	0	theme	Oligosaccharyl	68:81	arg1	Activities					123:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	3	1	theme	convenient	710:719	arg1	substrates					737:746	convenient non-radioactive substrates	710:746	convenient non-radioactive substrates	710:746	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	8	2	theme	lipid	1506:1510	arg1	analogs					1515:1521	fluorescent lipid II analogs	1494:1521	fluorescent lipid II analogs	1494:1521	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	8	2	theme	lipid	1506:1510	arg1	substrates					1554:1563	convenient non-radioactive substrates	1527:1563	convenient non-radioactive substrates for investigating DLODP and DLODP-like activities	1527:1613	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	7	3	theme	lipid	1389:1393	arg1	hydrolysis					1375:1384	hydrolysis	1375:1384	hydrolysis of lipid II by a eukaryotic enzyme	1375:1419	To conclude, we show, for the first time, hydrolysis of lipid II by a eukaryotic enzyme.					
31174247	0	4	theme	Mammalian	58:66	arg1	Activities					123:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	1	5	theme	PP	358:359	arg1	DLO					372:374	DLO	372:374	DLO	372:374	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	5	theme	PP	358:359	arg1	-dolichol					361:369	Glc3Man9GlcNAc2-diphospho (PP)-dolichol	331:369	Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO)	331:375	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	6	6	theme	DLODP	1317:1321	arg1	activity					1323:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	1	7	theme	mannose	273:279	arg1	N-acetylglucosamine					238:256	N-acetylglucosamine	238:256	N-acetylglucosamine	238:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	7	theme	mannose	273:279	arg1	residues					287:294	3 residues	285:294	3 residues of glucose (Glc3Man9 GlcNAc2)	285:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	7	theme	mannose	273:279	arg1	residues					261:268	9 residues	259:268	9 residues of mannose	259:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	7	theme	mannose	273:279	arg1	glucose					299:305	glucose	299:305	glucose (Glc3Man9 GlcNAc2)	299:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	7	theme	mannose	273:279	arg1	residues					226:233	2 residues	224:233	2 residues of N-acetylglucosamine	224:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	7	theme	mannose	273:279	arg1	mannose					273:279	mannose	273:279	mannose	273:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	0	8	theme	Diphosphatase	101:113	arg1	Activities					123:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	2	9	theme	unidentified	576:587	arg1	mechanisms					589:598	unidentified mechanisms	576:598	unidentified mechanisms	576:598	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	6	10	theme	biochemical	1225:1235	arg1	characteristics					1237:1251	similar biochemical characteristics	1217:1251	similar biochemical characteristics	1217:1251	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	8	11	theme	convenient	1527:1536	arg1	analogs					1515:1521	fluorescent lipid II analogs	1494:1521	fluorescent lipid II analogs	1494:1521	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	8	11	theme	convenient	1527:1536	arg1	substrates					1554:1563	convenient non-radioactive substrates	1527:1563	convenient non-radioactive substrates for investigating DLODP and DLODP-like activities	1527:1613	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	0	12	theme	Diphosphodolichol	83:99	arg1	Activities					123:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	8	13	theme	fluorescent	1494:1504	arg1	analogs					1515:1521	fluorescent lipid II analogs	1494:1521	fluorescent lipid II analogs	1494:1521	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	8	13	theme	fluorescent	1494:1504	arg1	substrates					1554:1563	convenient non-radioactive substrates	1527:1563	convenient non-radioactive substrates for investigating DLODP and DLODP-like activities	1527:1613	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	6	14	theme	HepG2	1306:1310	arg1	activity					1323:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	5	15	theme	mouse	971:975	arg1	extracts					992:999	mouse liver membrane extracts	971:999	mouse liver membrane extracts	971:999	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	16	theme	mass	932:935	arg1	spectrometry					937:948	mass spectrometry	932:948	mass spectrometry	932:948	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	0	17	theme	DLODP	116:120	arg1	Activities					123:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities	58:132	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	5	18	theme	GlcNAc-MurNAc	1109:1121	arg1	-P					1143:1144	GlcNAc-MurNAc(dansyl-pentapeptide)-P	1109:1144	GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P)	1109:1151	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	18	theme	GlcNAc-MurNAc	1109:1121	arg1	GM5P					1147:1150	GM5P	1147:1150	GM5P	1147:1150	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	6	19	theme	carcinoma	1296:1304	arg1	activity					1323:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	5	20	theme	liver	977:981	arg1	extracts					992:999	mouse liver membrane extracts	971:999	mouse liver membrane extracts	971:999	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	21	theme	extraction	862:871	arg1	procedure					873:881	a vancomycin-based solid-phase extraction procedure	831:881	a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry	831:948	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	1	22	from	proteins	382:389	arg1	reticulum					410:418	the endoplasmic reticulum	394:418	the endoplasmic reticulum (ER)	394:423	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	22	from	proteins	382:389	arg1	ER					421:422	ER	421:422	ER	421:422	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	2	23	theme	DLO	468:470	arg1	biosynthesis					472:483	DLO biosynthesis	468:483	DLO biosynthesis	468:483	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	5	24	theme	membrane	983:990	arg1	extracts					992:999	mouse liver membrane extracts	971:999	mouse liver membrane extracts	971:999	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	2	25	theme	truncated	503:511	arg1	DLO					513:515	truncated DLO	503:515	truncated DLO	503:515	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	0	26	theme	Lipid	10:14	arg1	Analogs					19:25	Bacterial Lipid II Analogs	0:25	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	5	27	theme	dansyl-pentapeptide	1123:1141	arg1	-P					1143:1144	GlcNAc-MurNAc(dansyl-pentapeptide)-P	1109:1144	GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P)	1109:1151	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	27	theme	dansyl-pentapeptide	1123:1141	arg1	GM5P					1147:1150	GM5P	1147:1150	GM5P	1147:1150	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	6	28	theme	human	1275:1279	arg1	carcinoma					1296:1304	human hepatocellular carcinoma	1275:1304	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	7	29	theme	eukaryotic	1403:1412	arg1	enzyme					1414:1419	a eukaryotic enzyme	1401:1419	a eukaryotic enzyme	1401:1419	To conclude, we show, for the first time, hydrolysis of lipid II by a eukaryotic enzyme.					
31174247	0	30	theme	Bacterial	0:8	arg1	Analogs					19:25	Bacterial Lipid II Analogs	0:25	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	3	31	theme	substrates	737:746	arg1	lack					702:705	a lack	700:705	a lack of convenient non-radioactive substrates	700:746	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	5	32	theme	dansyl-pentapeptide	1062:1080	arg1	-PP-undecaprenol					1082:1097	GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1048:1097	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	6	33	theme	GM5P	1154:1157	arg1	production					1159:1168	GM5P production	1154:1168	GM5P production by solubilized liver microsomal proteins	1154:1209	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	1	34	theme	oligosaccharide	197:211	arg1	transfer					182:189	the transfer	178:189	the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER)	178:423	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	5	35	theme	fluorescent	1011:1021	arg1	II					1039:1040	fluorescent bacterial lipid II	1011:1040	fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P)	1011:1151	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	35	theme	fluorescent	1011:1021	arg1	LII					1043:1045	LII	1043:1045	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	2	36	theme	oligosaccharyl	540:553	arg1	OSP					567:569	OSP	567:569	OSP	567:569	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	2	36	theme	oligosaccharyl	540:553	arg1	phosphates					555:564	oligosaccharyl phosphates	540:564	oligosaccharyl phosphates (OSP)	540:570	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	7	37	theme	first	1363:1367	arg1	time					1369:1372	the first time	1359:1372	the first time	1359:1372	To conclude, we show, for the first time, hydrolysis of lipid II by a eukaryotic enzyme.					
31174247	5	38	theme	lipid	1033:1037	arg1	II					1039:1040	fluorescent bacterial lipid II	1011:1040	fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P)	1011:1151	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	38	theme	lipid	1033:1037	arg1	LII					1043:1045	LII	1043:1045	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	0	39	theme	II	16:17	arg1	Analogs					19:25	Bacterial Lipid II Analogs	0:25	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	1	40	theme	glucose	299:305	arg1	N-acetylglucosamine					238:256	N-acetylglucosamine	238:256	N-acetylglucosamine	238:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	40	theme	glucose	299:305	arg1	residues					287:294	3 residues	285:294	3 residues of glucose (Glc3Man9 GlcNAc2)	285:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	40	theme	glucose	299:305	arg1	residues					261:268	9 residues	259:268	9 residues of mannose	259:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	40	theme	glucose	299:305	arg1	glucose					299:305	glucose	299:305	glucose (Glc3Man9 GlcNAc2)	299:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	40	theme	glucose	299:305	arg1	residues					226:233	2 residues	224:233	2 residues of N-acetylglucosamine	224:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	40	theme	glucose	299:305	arg1	mannose					273:279	mannose	273:279	mannose	273:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	6	41	theme	solubilized	1173:1183	arg1	proteins					1202:1209	solubilized liver microsomal proteins	1173:1209	solubilized liver microsomal proteins	1173:1209	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	6	42	theme	microsomal	1191:1200	arg1	proteins					1202:1209	solubilized liver microsomal proteins	1173:1209	solubilized liver microsomal proteins	1173:1209	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	8	43	theme	non-radioactive	1538:1552	arg1	analogs					1515:1521	fluorescent lipid II analogs	1494:1521	fluorescent lipid II analogs	1494:1521	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	8	43	theme	non-radioactive	1538:1552	arg1	substrates					1554:1563	convenient non-radioactive substrates	1527:1563	convenient non-radioactive substrates for investigating DLODP and DLODP-like activities	1527:1613	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	5	44	theme	thin	896:899	arg1	chromatography					907:920	thin layer chromatography	896:920	thin layer chromatography (TLC)	896:926	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	44	theme	thin	896:899	arg1	TLC					923:925	TLC	923:925	TLC	923:925	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	0	45	theme	Novel	28:32	arg1	Substrates					43:52	Novel In Vitro Substrates	28:52	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	3	46	theme	non-radioactive	721:735	arg1	substrates					737:746	convenient non-radioactive substrates	710:746	convenient non-radioactive substrates	710:746	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	4	47	theme	DLO	809:811	arg1	hydrolysis					813:822	DLO hydrolysis	809:822	DLO hydrolysis	809:822	Our objective was to develop a fluorescence-based assay for DLO hydrolysis.					
31174247	5	48	theme	vancomycin-based	833:848	arg1	procedure					873:881	a vancomycin-based solid-phase extraction procedure	831:881	a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry	831:948	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	1	49	theme	Glc3Man9	308:315	arg1	glucose					299:305	glucose	299:305	glucose (Glc3Man9 GlcNAc2)	299:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	49	theme	Glc3Man9	308:315	arg1	GlcNAc2					317:323	Glc3Man9 GlcNAc2	308:323	Glc3Man9 GlcNAc2	308:323	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	0	50	dep	In	34:35	arg1	Vitro					37:41	Vitro	37:41	Vitro	37:41	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	5	51	theme	solid-phase	850:860	arg1	procedure					873:881	a vancomycin-based solid-phase extraction procedure	831:881	a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry	831:948	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	8	52	theme	same	1459:1462	arg1	enzymes					1485:1491	the same, or closely related, enzymes	1455:1491	the same, or closely related, enzymes	1455:1491	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	5	53	theme	bacterial	1023:1031	arg1	II					1039:1040	fluorescent bacterial lipid II	1011:1040	fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P)	1011:1151	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	53	theme	bacterial	1023:1031	arg1	LII					1043:1045	LII	1043:1045	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	0	54	theme	In	34:35	arg1	Substrates					43:52	Novel In Vitro Substrates	28:52	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	6	55	theme	liver	1185:1189	arg1	proteins					1202:1209	solubilized liver microsomal proteins	1173:1209	solubilized liver microsomal proteins	1173:1209	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	8	56	theme	related	1476:1482	arg1	enzymes					1485:1491	the same, or closely related, enzymes	1455:1491	the same, or closely related, enzymes	1455:1491	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	1	57	theme	endoplasmic	398:408	arg1	reticulum					410:418	the endoplasmic reticulum	394:418	the endoplasmic reticulum (ER)	394:423	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	57	theme	endoplasmic	398:408	arg1	ER					421:422	ER	421:422	ER	421:422	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	6	58	theme	hepatocellular	1281:1294	arg1	carcinoma					1296:1304	human hepatocellular carcinoma	1275:1304	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	6	59	theme	cell	1312:1315	arg1	activity					1323:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	human hepatocellular carcinoma HepG2 cell DLODP activity	1275:1330	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	4	60	theme	fluorescence-based	780:797	arg1	assay					799:803	a fluorescence-based assay	778:803	a fluorescence-based assay for DLO hydrolysis	778:822	Our objective was to develop a fluorescence-based assay for DLO hydrolysis.					
31174247	5	61	theme	GlcNAc-MurNAc	1048:1060	arg1	-PP-undecaprenol					1082:1097	GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1048:1097	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	0	62	dep	Analogs	19:25	arg1	Substrates					43:52	Novel In Vitro Substrates	28:52	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.	0:133	Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	N-acetylglucosamine					238:256	N-acetylglucosamine	238:256	N-acetylglucosamine	238:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	residues					287:294	3 residues	285:294	3 residues of glucose (Glc3Man9 GlcNAc2)	285:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	residues					261:268	9 residues	259:268	9 residues of mannose	259:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	glucose					299:305	glucose	299:305	glucose (Glc3Man9 GlcNAc2)	299:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	residues					226:233	2 residues	224:233	2 residues of N-acetylglucosamine	224:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	63	theme	N-acetylglucosamine	238:256	arg1	mannose					273:279	mannose	273:279	mannose	273:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	6	64	theme	similar	1217:1223	arg1	characteristics					1237:1251	similar biochemical characteristics	1217:1251	similar biochemical characteristics	1217:1251	GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity.					
31174247	1	65	theme	Mammalian	135:143	arg1	N-glycosylation					153:167	Mammalian protein N-glycosylation	135:167	Mammalian protein N-glycosylation	135:167	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	2	66	theme	pathophysiological	437:454	arg1	conditions					456:465	some pathophysiological conditions	432:465	some pathophysiological conditions	432:465	Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms.					
31174247	8	67	theme	DLODP-like	1593:1602	arg1	activities					1604:1613	DLODP-like activities	1593:1613	DLODP-like activities	1593:1613	As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.					
31174247	1	68	contain	containing	213:222	arg2	residues					287:294	3 residues	285:294	3 residues of glucose (Glc3Man9 GlcNAc2)	285:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg2	mannose					273:279	mannose	273:279	mannose	273:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg1	oligosaccharide					197:211	an oligosaccharide	194:211	an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO)	194:375	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg2	glucose					299:305	glucose	299:305	glucose (Glc3Man9 GlcNAc2)	299:324	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg2	residues					226:233	2 residues	224:233	2 residues of N-acetylglucosamine	224:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg2	N-acetylglucosamine					238:256	N-acetylglucosamine	238:256	N-acetylglucosamine	238:256	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	68	contain	containing	213:222	arg2	residues					261:268	9 residues	259:268	9 residues of mannose	259:279	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	3	69	theme	DLO	601:603	arg1	DLODP					629:633	DLODP	629:633	DLODP	629:633	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	3	69	theme	DLO	601:603	arg1	activity					619:626	DLO diphosphatase activity	601:626	DLO diphosphatase activity (DLODP)	601:634	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	1	70	theme	protein	145:151	arg1	N-glycosylation					153:167	Mammalian protein N-glycosylation	135:167	Mammalian protein N-glycosylation	135:167	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	5	71	dep	LII	1043:1045	arg1	-PP-undecaprenol					1082:1097	GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1048:1097	LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol	1043:1097	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	1	72	theme	Glc3Man9GlcNAc2-diphospho	331:355	arg1	DLO					372:374	DLO	372:374	DLO	372:374	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	1	72	theme	Glc3Man9GlcNAc2-diphospho	331:355	arg1	-dolichol					361:369	Glc3Man9GlcNAc2-diphospho (PP)-dolichol	331:369	Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO)	331:375	Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER).					
31174247	3	73	theme	diphosphatase	605:617	arg1	DLODP					629:633	DLODP	629:633	DLODP	629:633	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	3	73	theme	diphosphatase	605:617	arg1	activity					619:626	DLO diphosphatase activity	601:626	DLO diphosphatase activity (DLODP)	601:634	DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates.					
31174247	5	74	theme	layer	901:905	arg1	chromatography					907:920	thin layer chromatography	896:920	thin layer chromatography (TLC)	896:926	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
31174247	5	74	theme	layer	901:905	arg1	TLC					923:925	TLC	923:925	TLC	923:925	Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P).					
29532951	4	0	from	Studies	573:579	arg1	bean					599:602	bean	599:602	bean	599:602	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	4	0	from	Studies	573:579	arg1	lupin					608:612	lupin	608:612	lupin	608:612	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	4	0	from	Studies	573:579	arg1	pulses					584:589	pulses	584:589	pulses such as bean and lupin	584:612	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	3	1	theme	downstream	472:481	arg1	processing					483:492	downstream processing	472:492	downstream processing	472:492	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	5	2	theme	abundance	936:944	arg1	details					886:892	details	886:892	details of polysaccharide composition, structure, abundance, and location	886:958	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	0	3	dep	Cicer	108:112	arg1	L.					124:125	Cicer arietinum L.	108:125	Cicer arietinum L.	108:125	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	8	4	theme	important	1437:1445	arg1	role					1447:1450	a potentially important role	1423:1450	a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea	1423:1565	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	0	5	theme	desi	93:96	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	0	5	theme	desi	93:96	arg1	cotyledons					128:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	1	6	dep	Cicer	150:154	arg1	L.					166:167	Cicer arietinum L.	150:167	Cicer arietinum L.	150:167	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	4	7	theme	desi	728:731	arg1	composition					752:762	desi chickpea cotyledon composition	728:762	desi chickpea cotyledon composition	728:762	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	4	8	theme	underlying	696:705	arg1	relationship					707:718	the underlying relationship	692:718	the underlying relationship between desi chickpea cotyledon composition and cooking performance	692:786	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	0	9	theme	mature	86:91	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	0	9	theme	mature	86:91	arg1	cotyledons					128:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	8	10	theme	field	1399:1403	arg1	sites					1405:1409	distinct field sites	1390:1409	distinct field sites	1390:1409	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	5	11	used	utilized	814:821	arg2	we					811:812	we	811:812	we	811:812	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	5	12	theme	composition	912:922	arg1	details					886:892	details	886:892	details of polysaccharide composition, structure, abundance, and location	886:958	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	0	13	from	variation	48:56	arg1	composition					71:81	cell wall composition	61:81	cell wall composition	61:81	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	0	13	from	variation	48:56	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	0	13	from	variation	48:56	arg1	cotyledons					128:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	6	14	theme	abundant	1033:1040	arg1	components					1052:1061	the most abundant cell wall components	1024:1061	the most abundant cell wall components	1024:1061	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	14	theme	abundant	1033:1040	arg1	polysaccharides					1003:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	4	15	theme	cotyledon	742:750	arg1	composition					752:762	desi chickpea cotyledon composition	728:762	desi chickpea cotyledon composition	728:762	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	3	16	theme	desi	405:408	arg1	genotypes					410:418	desi genotypes	405:418	desi genotypes	405:418	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	0	17	theme	chickpea	98:105	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	0	17	theme	chickpea	98:105	arg1	cotyledons					128:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	mature desi chickpea (Cicer arietinum L.) cotyledons	86:137	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	6	18	theme	linkage	1113:1119	arg1	content					1121:1127	glycosidic linkage content	1102:1127	glycosidic linkage content	1102:1127	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	4	19	theme	chickpea	733:740	arg1	composition					752:762	desi chickpea cotyledon composition	728:762	desi chickpea cotyledon composition	728:762	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	6	20	from	differences	1068:1078	arg1	monosaccharide					1083:1096	monosaccharide	1083:1096	monosaccharide	1083:1096	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	20	from	differences	1068:1078	arg1	content					1121:1127	glycosidic linkage content	1102:1127	glycosidic linkage content	1102:1127	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	21	theme	genetic	1160:1166	arg1	factors					1168:1174	both environmental and genetic factors	1137:1174	both environmental and genetic factors	1137:1174	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	5	22	theme	chemical	836:843	arg1	assays					868:873	chemical and immunohistological assays	836:873	chemical and immunohistological assays	836:873	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	0	23	theme	Genetic	0:6	arg1	factors					26:32	Genetic and environmental factors	0:32	Genetic and environmental factors	0:32	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	5	24	theme	structure	925:933	arg1	details					886:892	details	886:892	details of polysaccharide composition, structure, abundance, and location	886:958	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	6	25	theme	wall	1047:1050	arg1	components					1052:1061	the most abundant cell wall components	1024:1061	the most abundant cell wall components	1024:1061	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	25	theme	wall	1047:1050	arg1	polysaccharides					1003:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	8	26	theme	regulatory	1503:1512	arg1	machinery					1514:1522	their underlying regulatory machinery	1486:1522	their underlying regulatory machinery	1486:1522	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	7	27	theme	pectin	1282:1287	arg1	methylesterification					1289:1308	pectin methylesterification	1282:1308	pectin methylesterification	1282:1308	Genotype-specific differences were identified in arabinan structure, pectin methylesterification, and calcium-mediated pectin dimerization.					
29532951	2	28	theme	chickpea	268:275	arg1	seeds					277:281	desi chickpea seeds	263:281	desi chickpea seeds	263:281	Prior to cooking, desi chickpea seeds are most often dehulled and cleaved to release the split cotyledons, referred to as dhal.					
29532951	8	29	theme	cell	1456:1459	arg1	polysaccharides					1466:1480	cell wall polysaccharides	1456:1480	cell wall polysaccharides	1456:1480	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	3	30	theme	significant	426:436	arg1	impact					438:443	a significant impact	424:443	a significant impact	424:443	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	0	31	theme	environmental	12:24	arg1	factors					26:32	Genetic and environmental factors	0:32	Genetic and environmental factors	0:32	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	5	32	theme	location	951:958	arg1	details					886:892	details	886:892	details of polysaccharide composition, structure, abundance, and location	886:958	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	6	33	theme	cotyledon	1190:1198	arg1	composition					1200:1210	cotyledon composition	1190:1210	cotyledon composition	1190:1210	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	2	34	theme	desi	263:266	arg1	seeds					277:281	desi chickpea seeds	263:281	desi chickpea seeds	263:281	Prior to cooking, desi chickpea seeds are most often dehulled and cleaved to release the split cotyledons, referred to as dhal.					
29532951	4	35	theme	wall	640:643	arg1	polysaccharides					645:659	cell wall polysaccharides	635:659	cell wall polysaccharides	635:659	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	7	36	theme	Genotype-specific	1213:1229	arg1	differences					1231:1241	Genotype-specific differences	1213:1241	Genotype-specific differences	1213:1241	Genotype-specific differences were identified in arabinan structure, pectin methylesterification, and calcium-mediated pectin dimerization.					
29532951	3	37	contain	has	420:422	arg2	impact					438:443	a significant impact	424:443	a significant impact	424:443	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	3	37	contain	has	420:422	arg1	variation					387:395	Compositional variation	373:395	Compositional variation between desi genotypes	373:418	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	3	38	theme	protein	556:562	arg1	content					564:570	protein content	556:570	protein content	556:570	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	1	39	theme	important	176:184	arg1	crop					212:215	an important nutritionally rich legume crop	173:215	an important nutritionally rich legume crop that is consumed worldwide	173:242	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	1	39	theme	important	176:184	arg1	Chickpea					140:147	Chickpea	140:147	Chickpea (Cicer arietinum L.)	140:168	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	3	40	theme	Compositional	373:385	arg1	variation					387:395	Compositional variation	373:395	Compositional variation between desi genotypes	373:418	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	3	41	theme	content	564:570	arg1	terms					536:540	terms	536:540	terms of starch and protein content	536:570	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	5	42	theme	immunohistological	849:866	arg1	assays					868:873	chemical and immunohistological assays	836:873	chemical and immunohistological assays	836:873	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	7	43	theme	arabinan	1262:1269	arg1	structure					1271:1279	arabinan structure	1262:1279	arabinan structure	1262:1279	Genotype-specific differences were identified in arabinan structure, pectin methylesterification, and calcium-mediated pectin dimerization.					
29532951	3	44	theme	starch	545:550	arg1	terms					536:540	terms	536:540	terms of starch and protein content	536:570	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	4	45	theme	time	672:675	arg1	variation					677:685	time variation	672:685	time variation	672:685	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	6	46	theme	environmental	1142:1154	arg1	factors					1168:1174	both environmental and genetic factors	1137:1174	both environmental and genetic factors	1137:1174	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	7	47	theme	calcium-mediated	1315:1330	arg1	dimerization					1339:1350	calcium-mediated pectin dimerization	1315:1350	calcium-mediated pectin dimerization	1315:1350	Genotype-specific differences were identified in arabinan structure, pectin methylesterification, and calcium-mediated pectin dimerization.					
29532951	7	48	theme	pectin	1332:1337	arg1	dimerization					1339:1350	calcium-mediated pectin dimerization	1315:1350	calcium-mediated pectin dimerization	1315:1350	Genotype-specific differences were identified in arabinan structure, pectin methylesterification, and calcium-mediated pectin dimerization.					
29532951	8	49	theme	underlying	1492:1501	arg1	machinery					1514:1522	their underlying regulatory machinery	1486:1522	their underlying regulatory machinery	1486:1522	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	8	50	from	role	1447:1450	arg1	control					1531:1537	the control	1527:1537	the control of cooking time in chickpea	1527:1565	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	1	51	dep	important	176:184	arg1	rich					200:203	rich	200:203	rich	200:203	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	5	52	theme	polysaccharide	897:910	arg1	composition					912:922	polysaccharide composition	897:922	polysaccharide composition	897:922	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	6	53	theme	glycosidic	1102:1111	arg1	content					1121:1127	glycosidic linkage content	1102:1127	glycosidic linkage content	1102:1127	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	5	54	theme	desi	971:974	arg1	cotyledon					985:993	the desi chickpea cotyledon	967:993	the desi chickpea cotyledon	967:993	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	3	55	theme	nutritional	448:458	arg1	quality					460:466	nutritional quality	448:466	nutritional quality	448:466	Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content.					
29532951	8	56	theme	distinct	1390:1397	arg1	sites					1405:1409	distinct field sites	1390:1409	distinct field sites	1390:1409	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29532951	1	57	theme	legume	205:210	arg1	crop					212:215	an important nutritionally rich legume crop	173:215	an important nutritionally rich legume crop that is consumed worldwide	173:242	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	1	57	theme	legume	205:210	arg1	Chickpea					140:147	Chickpea	140:147	Chickpea (Cicer arietinum L.)	140:168	Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide.					
29532951	0	58	theme	wall	66:69	arg1	composition					71:81	cell wall composition	61:81	cell wall composition	61:81	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	5	59	theme	assays	868:873	arg1	assays					868:873	chemical and immunohistological assays	836:873	chemical and immunohistological assays	836:873	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	5	59	theme	assays	868:873	arg1	variety					825:831	a variety	823:831	a variety of chemical and immunohistological assays	823:873	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	5	60	theme	chickpea	976:983	arg1	cotyledon					985:993	the desi chickpea cotyledon	967:993	the desi chickpea cotyledon	967:993	Here, we utilized a variety of chemical and immunohistological assays to examine details of polysaccharide composition, structure, abundance, and location within the desi chickpea cotyledon.					
29532951	6	61	theme	cell	1042:1045	arg1	components					1052:1061	the most abundant cell wall components	1024:1061	the most abundant cell wall components	1024:1061	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	61	theme	cell	1042:1045	arg1	polysaccharides					1003:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	2	62	theme	split	334:338	arg1	cotyledons					340:349	the split cotyledons	330:349	the split cotyledons	330:349	Prior to cooking, desi chickpea seeds are most often dehulled and cleaved to release the split cotyledons, referred to as dhal.					
29532951	6	63	theme	Pectic	996:1001	arg1	components					1052:1061	the most abundant cell wall components	1024:1061	the most abundant cell wall components	1024:1061	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	6	63	theme	Pectic	996:1001	arg1	polysaccharides					1003:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides	996:1017	Pectic polysaccharides were the most abundant cell wall components, and differences in monosaccharide and glycosidic linkage content suggest both environmental and genetic factors contribute to cotyledon composition.					
29532951	0	64	theme	cell	61:64	arg1	composition					71:81	cell wall composition	61:81	cell wall composition	61:81	Genetic and environmental factors contribute to variation in cell wall composition in mature desi chickpea (Cicer arietinum L.) cotyledons.					
29532951	4	65	theme	cooking	768:774	arg1	performance					776:786	cooking performance	768:786	cooking performance	768:786	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	4	66	theme	cell	635:638	arg1	polysaccharides					645:659	cell wall polysaccharides	635:659	cell wall polysaccharides	635:659	Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear.					
29532951	8	67	theme	wall	1461:1464	arg1	polysaccharides					1466:1480	cell wall polysaccharides	1456:1480	cell wall polysaccharides	1456:1480	These differences were replicated in distinct field sites and suggest a potentially important role for cell wall polysaccharides and their underlying regulatory machinery in the control of cooking time in chickpea.					
29572115	9	0	theme	glycome	1323:1329	arg1	composition					1331:1341	IgG glycome composition	1319:1341	IgG glycome composition	1319:1341	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	5	1	theme	IgG	864:866	arg1	glycans					868:874	IgG glycans	864:874	IgG glycans	864:874	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	4	2	from	traits	658:663	arg1	subjects					672:679	179 subjects	668:679	179 subjects who developed RA within 10-years follow-up and 358 matched controls	668:747	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	9	3	theme	RA	1382:1383	arg1	mechanisms					1368:1377	the underlying mechanisms	1353:1377	the underlying mechanisms of RA	1353:1383	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	3	theme	RA	1382:1383	arg1	factor					1443:1448	a pre-existing risk factor	1423:1448	a pre-existing risk factor involved in the disease development	1423:1484	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	3	theme	RA	1382:1383	arg1	galactosylation					1404:1418	decreased galactosylation	1394:1418	decreased galactosylation	1394:1418	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	2	4	theme	RA	324:325	arg1	patients					327:334	Most RA patients	319:334	Most RA patients	319:334	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	0	5	theme	arthritis	90:98	arg1	diagnosis					66:74	future diagnosis	59:74	future diagnosis of rheumatoid arthritis	59:98	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	6	6	theme	RA	884:885	arg1	diagnosis					887:895	Future RA diagnosis	877:895	Future RA diagnosis	877:895	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	6	7	theme	Future	877:882	arg1	diagnosis					887:895	Future RA diagnosis	877:895	Future RA diagnosis	877:895	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	4	8	theme	10-years	705:712	arg1	follow-up					714:722	10-years follow-up	705:722	10-years follow-up	705:722	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	0	9	theme	rheumatoid	79:88	arg1	arthritis					90:98	rheumatoid arthritis	79:98	rheumatoid arthritis	79:98	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	1	10	contain	have	154:157	arg1	Antibodies					130:139	Antibodies	130:139	Antibodies	130:139	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	10	contain	have	154:157	arg2	role					172:175	an important role	159:175	an important role	159:175	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	4	11	theme	IgG	567:569	arg1	glycosylation					571:583	IgG glycosylation	567:583	IgG glycosylation in RA	567:589	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	3	12	theme	altered	498:504	arg1	glycosylation					510:522	altered IgG glycosylation	498:522	altered IgG glycosylation	498:522	We undertook this study to determine whether altered IgG glycosylation precedes the disease diagnosis.					
29572115	5	13	theme	chromatography	775:788	arg1	method					790:795	Ultra-performance liquid chromatography method	750:795	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC)	750:842	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	3	14	theme	IgG	506:508	arg1	glycosylation					510:522	altered IgG glycosylation	498:522	altered IgG glycosylation	498:522	We undertook this study to determine whether altered IgG glycosylation precedes the disease diagnosis.					
29572115	1	15	theme	important	162:170	arg1	role					172:175	an important role	159:175	an important role	159:175	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	9	16	theme	IgG	1319:1321	arg1	composition					1331:1341	IgG glycome composition	1319:1341	IgG glycome composition	1319:1341	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	6	17	theme	matched	1008:1014	arg1	controls					1016:1023	the matched controls	1004:1023	the matched controls	1004:1023	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	2	18	theme	immunoglobulin	367:380	arg1	G					382:382	immunoglobulin G	367:382	immunoglobulin G (IgG)	367:388	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	2	18	theme	immunoglobulin	367:380	arg1	IgG					385:387	IgG	385:387	IgG	385:387	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	0	19	theme	Low	0:2	arg1	galactosylation					4:18	Low galactosylation	0:18	Low galactosylation of IgG	0:25	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	9	20	theme	long-acting	1289:1299	arg1	factors					1301:1307	long-acting factors	1289:1307	long-acting factors affecting IgG glycome composition	1289:1341	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	8	21	theme	IgG	1193:1195	arg1	glycosylation					1197:1209	IgG glycosylation	1193:1209	IgG glycosylation	1193:1209	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	2	22	theme	IgG	405:407	arg1	glycosylation					409:421	IgG glycosylation	405:421	IgG glycosylation	405:421	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	1	23	theme	prevalent	242:250	arg1	diseases					273:280	the most prevalent chronic inflammatory diseases	233:280	the most prevalent chronic inflammatory diseases	233:280	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	0	24	theme	follow-up	119:127	arg1	10 years					107:114	10 years	107:114	10 years of follow-up	107:127	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	4	25	gly	glycosylation	571:583	arg1	RA					588:589	RA	588:589	RA	588:589	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	4	25	gly	glycosylation	571:583	arg1	N = 14,749					619:628	N = 14,749	619:628	N = 14,749	619:628	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	4	25	gly	glycosylation	571:583	arg1	cohorts					610:616	two prospective cohorts	594:616	two prospective cohorts (N = 14,749)	594:629	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	5	26	used	used	848:851	arg2	method					790:795	Ultra-performance liquid chromatography method	750:795	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC)	750:842	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	9	27	theme	risk	1438:1441	arg1	factor					1443:1448	a pre-existing risk factor	1423:1448	a pre-existing risk factor involved in the disease development	1423:1484	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	27	theme	risk	1438:1441	arg1	galactosylation					1404:1418	decreased galactosylation	1394:1418	decreased galactosylation	1394:1418	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	5	28	theme	hydrophilic	806:816	arg1	HILIC-UPLC					832:841	HILIC-UPLC	832:841	HILIC-UPLC	832:841	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	5	28	theme	hydrophilic	806:816	arg1	interactions					818:829	hydrophilic interactions	806:829	hydrophilic interactions (HILIC-UPLC)	806:842	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	1	29	theme	chronic	252:258	arg1	diseases					273:280	the most prevalent chronic inflammatory diseases	233:280	the most prevalent chronic inflammatory diseases	233:280	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	3	30	theme	disease	537:543	arg1	diagnosis					545:553	the disease diagnosis	533:553	the disease diagnosis	533:553	We undertook this study to determine whether altered IgG glycosylation precedes the disease diagnosis.					
29572115	9	31	theme	decreased	1394:1402	arg1	factor					1443:1448	a pre-existing risk factor	1423:1448	a pre-existing risk factor involved in the disease development	1423:1484	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	31	theme	decreased	1394:1402	arg1	galactosylation					1404:1418	decreased galactosylation	1394:1418	decreased galactosylation	1394:1418	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	4	32	theme	prospective	598:608	arg1	N = 14,749					619:628	N = 14,749	619:628	N = 14,749	619:628	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	4	32	theme	prospective	598:608	arg1	cohorts					610:616	two prospective cohorts	594:616	two prospective cohorts (N = 14,749)	594:629	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	7	33	theme	median	1150:1155	arg1	RA					1146:1147	RA	1146:1147	RA (median time 4.31 years)	1146:1172	In RA cases, these traits did not correlate with the time between being recruited to the study and being diagnosed with RA (median time 4.31 years).					
29572115	7	33	theme	median	1150:1155	arg1	4.31 years					1162:1171	median time 4.31 years	1150:1171	median time 4.31 years	1150:1171	In RA cases, these traits did not correlate with the time between being recruited to the study and being diagnosed with RA (median time 4.31 years).					
29572115	1	34	theme	inflammatory	260:271	arg1	diseases					273:280	the most prevalent chronic inflammatory diseases	233:280	the most prevalent chronic inflammatory diseases	233:280	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	9	35	theme	disease	1466:1472	arg1	development					1474:1484	the disease development	1462:1484	the disease development	1462:1484	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	36	theme	pre-existing	1425:1436	arg1	factor					1443:1448	a pre-existing risk factor	1423:1448	a pre-existing risk factor involved in the disease development	1423:1484	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	9	36	theme	pre-existing	1425:1436	arg1	galactosylation					1404:1418	decreased galactosylation	1394:1418	decreased galactosylation	1394:1418	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	5	37	theme	Ultra-performance	750:766	arg1	method					790:795	Ultra-performance liquid chromatography method	750:795	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC)	750:842	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	1	38	theme	diseases	273:280	arg1	one					226:228	one	226:228	one	226:228	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	38	theme	diseases	273:280	arg1	arthritis					210:218	rheumatoid arthritis	199:218	rheumatoid arthritis (RA)	199:223	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	38	theme	diseases	273:280	arg1	diseases					273:280	the most prevalent chronic inflammatory diseases	233:280	the most prevalent chronic inflammatory diseases	233:280	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	6	39	dep	lower	931:935	arg1	sialylation					957:967	sialylation	957:967	sialylation	957:967	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	6	39	dep	lower	931:935	arg1	galactosylation					937:951	galactosylation	937:951	galactosylation	937:951	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	0	40	theme	IgG	23:25	arg1	galactosylation					4:18	Low galactosylation	0:18	Low galactosylation of IgG	0:25	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	1	41	theme	rheumatoid	199:208	arg1	RA					221:222	RA	221:222	RA	221:222	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	41	theme	rheumatoid	199:208	arg1	one					226:228	one	226:228	one	226:228	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	41	theme	rheumatoid	199:208	arg1	arthritis					210:218	rheumatoid arthritis	199:218	rheumatoid arthritis (RA)	199:223	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	1	41	theme	rheumatoid	199:208	arg1	diseases					273:280	the most prevalent chronic inflammatory diseases	233:280	the most prevalent chronic inflammatory diseases	233:280	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	5	42	theme	liquid	768:773	arg1	method					790:795	Ultra-performance liquid chromatography method	750:795	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC)	750:842	Ultra-performance liquid chromatography method based on hydrophilic interactions (HILIC-UPLC) was used to analyse IgG glycans.					
29572115	4	43	from	glycosylation	571:583	arg1	RA					588:589	RA	588:589	RA	588:589	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	2	44	theme	Most	319:322	arg1	patients					327:334	Most RA patients	319:334	Most RA patients	319:334	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	0	45	theme	higher	43:48	arg1	risk					50:53	higher risk	43:53	higher risk for future diagnosis of rheumatoid arthritis	43:98	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	6	46	theme	related	920:926	arg1	traits					913:918	traits	913:918	traits related to lower galactosylation and sialylation of IgG	913:974	Future RA diagnosis associated with traits related to lower galactosylation and sialylation of IgG when comparing the cases to the matched controls.					
29572115	7	47	theme	RA	1029:1030	arg1	cases					1032:1036	RA cases	1029:1036	RA cases	1029:1036	In RA cases, these traits did not correlate with the time between being recruited to the study and being diagnosed with RA (median time 4.31 years).					
29572115	8	48	theme	present	1237:1243	arg1	difference					1179:1188	The difference	1175:1188	The difference in IgG glycosylation	1175:1209	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	8	48	theme	present	1237:1243	arg1	years					1245:1249	relatively stable and present years	1215:1249	relatively stable and present years	1215:1249	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	8	49	from	difference	1179:1188	arg1	glycosylation					1197:1209	IgG glycosylation	1193:1209	IgG glycosylation	1193:1209	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	1	50	theme	arthritis	210:218	arg1	development					184:194	the development	180:194	the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints	180:316	Antibodies are known to have an important role in the development of rheumatoid arthritis (RA), one of the most prevalent chronic inflammatory diseases which primarily involves the joints.					
29572115	4	51	theme	matched	732:738	arg1	controls					740:747	358 matched controls	728:747	358 matched controls	728:747	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	9	52	theme	underlying	1357:1366	arg1	mechanisms					1368:1377	the underlying mechanisms	1353:1377	the underlying mechanisms of RA	1353:1383	This indicates that long-acting factors affecting IgG glycome composition are among the underlying mechanisms of RA and that decreased galactosylation is a pre-existing risk factor involved in the disease development.					
29572115	0	53	theme	future	59:64	arg1	diagnosis					66:74	future diagnosis	59:74	future diagnosis of rheumatoid arthritis	59:98	Low galactosylation of IgG associates with higher risk for future diagnosis of rheumatoid arthritis during 10 years of follow-up.					
29572115	8	54	theme	stable	1226:1231	arg1	difference					1179:1188	The difference	1175:1188	The difference in IgG glycosylation	1175:1209	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	8	54	theme	stable	1226:1231	arg1	years					1245:1249	relatively stable and present years	1215:1249	relatively stable and present years	1215:1249	The difference in IgG glycosylation was relatively stable and present years before diagnosis.					
29572115	4	55	theme	glycan	651:656	arg1	traits					658:663	28 IgG glycan traits	644:663	28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls	644:747	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	2	56	from	changes	394:400	arg1	glycosylation					409:421	IgG glycosylation	405:421	IgG glycosylation	405:421	Most RA patients develop autoantibodies against immunoglobulin G (IgG) and changes in IgG glycosylation have been associated with RA.					
29572115	4	57	theme	IgG	647:649	arg1	traits					658:663	28 IgG glycan traits	644:663	28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls	644:747	We studied IgG glycosylation in RA in two prospective cohorts (N = 14,749) by measuring 28 IgG glycan traits in 179 subjects who developed RA within 10-years follow-up and 358 matched controls.					
29572115	7	58	theme	time	1157:1160	arg1	RA					1146:1147	RA	1146:1147	RA (median time 4.31 years)	1146:1172	In RA cases, these traits did not correlate with the time between being recruited to the study and being diagnosed with RA (median time 4.31 years).					
29572115	7	58	theme	time	1157:1160	arg1	4.31 years					1162:1171	median time 4.31 years	1150:1171	median time 4.31 years	1150:1171	In RA cases, these traits did not correlate with the time between being recruited to the study and being diagnosed with RA (median time 4.31 years).					
30086262	12	0	theme	C1GALT1	1962:1968	arg1	knockout					1950:1957	knockout	1950:1957	knockout	1950:1957	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	7	1	theme	PDAC	1229:1232	arg1	cells					1234:1238	knockout PDAC cells	1220:1238	knockout PDAC cells	1220:1238	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	10	2	contain	had	1785:1787	arg2	production					1799:1808	increased production	1789:1808	increased production of Tn	1789:1814	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	2	contain	had	1785:1787	arg1	tumors					1636:1641	Pancreatic tumors	1625:1641	Pancreatic tumors that developed in KPCC mice	1625:1669	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	3	theme	Tn	1813:1814	arg1	production					1799:1808	increased production	1789:1808	increased production of Tn	1789:1814	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	4	theme	KPCC	1661:1664	arg1	mice					1666:1669	KPCC mice	1661:1669	KPCC mice	1661:1669	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	12	5	theme	Human	1928:1932	arg1	cells					1939:1943	Human PDAC cells	1928:1943	Human PDAC cells with knockout of C1GALT1	1928:1968	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	9	6	theme	survival	1422:1429	arg1	times					1431:1435	significantly shorter survival times	1400:1435	significantly shorter survival times (median 102 days)	1400:1453	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	6	theme	survival	1422:1429	arg1	days					1449:1452	median 102 days	1438:1452	median 102 days	1438:1452	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	7	theme	KPC	1615:1617	arg1	mice					1619:1622	KPC mice	1615:1622	KPC mice	1615:1622	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	13	8	with	mice	2142:2145	arg1	disruption					2152:2161	disruption	2152:2161	disruption of C1galt1	2152:2172	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	12	9	theme	genes	2062:2066	arg1	cells					2028:2032	control cells	2020:2032	control cells	2020:2032	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	12	9	theme	genes	2062:2066	arg1	expression					2048:2057	increased expression	2038:2057	increased expression of genes that regulate tumorigenesis and metastasis	2038:2109	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	14	10	theme	increased	2301:2309	arg1	tumorigenicity					2311:2324	increased tumorigenicity	2301:2324	increased tumorigenicity	2301:2324	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	14	11	theme	increased	2396:2404	arg1	aggressiveness					2406:2419	increased aggressiveness	2396:2419	increased aggressiveness	2396:2419	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	8	12	theme	C1GALT1	1241:1247	arg1	expression					1249:1258	C1GALT1 expression	1241:1258	C1GALT1 expression	1241:1258	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	2	13	theme	synthase	293:300	arg1	3-β-galactosyltransferase					337:361	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1	286:363	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1)	286:373	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	2	13	theme	synthase	293:300	arg1	C1GALT1					366:372	C1GALT1	366:372	C1GALT1	366:372	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	1	14	theme	truncated	187:195	arg1	structures					206:215	truncated O-glycan structures	187:215	truncated O-glycan structures (such as Tn and sTn)	187:236	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	6	15	used	used	941:944	arg2	We					938:939	We	938:939	We	938:939	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	2	16	theme	core	286:289	arg1	3-β-galactosyltransferase					337:361	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1	286:363	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1)	286:373	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	2	16	theme	core	286:289	arg1	C1GALT1					366:372	C1GALT1	366:372	C1GALT1	366:372	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	1	17	theme	structures	206:215	arg1	levels					177:182	higher levels	170:182	higher levels of truncated O-glycan structures (such as Tn and sTn)	170:236	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	1	18	theme	Pancreatic	120:129	arg1	PDACs					155:159	PDACs	155:159	PDACs	155:159	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	1	18	theme	Pancreatic	120:129	arg1	adenocarcinomas					138:152	Pancreatic ductal adenocarcinomas	120:152	Pancreatic ductal adenocarcinomas (PDACs)	120:160	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	12	19	contain	had	1970:1972	arg1	cells					1939:1943	Human PDAC cells	1928:1943	Human PDAC cells with knockout of C1GALT1	1928:1968	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	12	19	contain	had	1970:1972	arg2	glycosylation					1983:1995	aberrant glycosylation	1974:1995	aberrant glycosylation of MUC16	1974:2004	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	10	20	theme	KPC	1737:1739	arg1	mice					1741:1744	KPC mice	1737:1744	KPC mice	1737:1744	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	9	21	theme	KPC	1460:1462	arg1	mice					1464:1467	KPC mice	1460:1467	KPC mice (median 200 days)	1460:1485	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	21	theme	KPC	1460:1462	arg1	days					1481:1484	median 200 days	1470:1484	median 200 days	1470:1484	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	12	22	with	cells	1939:1943	arg1	knockout					1950:1957	knockout	1950:1957	knockout	1950:1957	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	11	23	theme	lower	1872:1876	arg1	levels					1878:1883	significantly lower levels	1858:1883	significantly lower levels of C1GALT1	1858:1894	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	5	24	theme	lectin	921:926	arg1	staining					928:935	lectin staining	921:935	lectin staining	921:935	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	9	25	from	weeks	1550:1554	arg1	PDAC					1557:1560	PDAC	1557:1560	PDAC at 5 weeks	1557:1571	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	25	from	weeks	1550:1554	arg1	neoplasias					1534:1543	early pancreatic intraepithelial neoplasias	1501:1543	early pancreatic intraepithelial neoplasias at 3 weeks	1501:1554	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	25	from	weeks	1550:1554	arg1	metastasis					1578:1587	metastasis	1578:1587	metastasis	1578:1587	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	11	26	theme	well-differentiated	1901:1919	arg1	PDACs					1921:1925	well-differentiated PDACs	1901:1925	well-differentiated PDACs	1901:1925	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	12	27	theme	control	2020:2026	arg1	cells					2028:2032	control cells	2020:2032	control cells	2020:2032	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	9	28	theme	median	1470:1475	arg1	mice					1464:1467	KPC mice	1460:1467	KPC mice (median 200 days)	1460:1485	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	28	theme	median	1470:1475	arg1	days					1481:1484	median 200 days	1470:1484	median 200 days	1470:1484	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	4	29	theme	KPCC	700:703	arg1	mice					705:708	KPCC mice	700:708	KPCC mice	700:708	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	9	30	theme	shorter	1414:1420	arg1	times					1431:1435	significantly shorter survival times	1400:1435	significantly shorter survival times (median 102 days)	1400:1453	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	30	theme	shorter	1414:1420	arg1	days					1449:1452	median 102 days	1438:1452	median 102 days	1438:1452	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	8	31	theme	differentiated	1316:1329	arg1	samples					1345:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	0	32	theme	Adenocarcinomas	77:91	arg1	Development					36:46	Development	36:46	Development	36:46	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	0	32	theme	Adenocarcinomas	77:91	arg1	Metastasis					52:61	Metastasis	52:61	Metastasis	52:61	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	6	33	theme	PDAC	997:1000	arg1	T3M4					1009:1012	T3M4	1009:1012	T3M4	1009:1012	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	33	theme	PDAC	997:1000	arg1	cells					1002:1006	human PDAC cells	991:1006	human PDAC cells (T3M4 and CD18/HPAF)	991:1027	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	33	theme	PDAC	997:1000	arg1	CD18/HPAF					1018:1026	CD18/HPAF	1018:1026	CD18/HPAF	1018:1026	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	8	34	theme	n =	1332:1334	arg1	samples					1345:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	5	35	theme	tumors	748:753	arg1	progression					722:732	progression	722:732	progression	722:732	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	5	35	theme	tumors	748:753	arg1	Growth					711:716	Growth	711:716	Growth	711:716	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	9	36	theme	pancreatic	1507:1516	arg1	neoplasias					1534:1543	early pancreatic intraepithelial neoplasias	1501:1543	early pancreatic intraepithelial neoplasias at 3 weeks	1501:1554	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	1	37	dep	AIMS	115:118	arg1	produce					162:168	produce	162:168	produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata	162:258	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	2	38	theme	truncated	414:422	arg1	O-glycans					424:432	these truncated O-glycans	408:432	these truncated O-glycans	408:432	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	12	39	gly	glycosylation	1983:1995	arg1	MUC16					2000:2004	MUC16	2000:2004	MUC16	2000:2004	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	7	40	theme	sequencing	1160:1169	arg1	analyses					1171:1178	RNA sequencing analyses	1156:1178	RNA sequencing analyses	1156:1178	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	6	41	theme	O-glycans	1043:1051	arg1	levels					1033:1038	levels	1033:1038	levels of O-glycans	1033:1051	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	7	42	dep	control	1200:1206	arg1	cells					1234:1238	knockout PDAC cells	1220:1238	knockout PDAC cells	1220:1238	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	10	43	theme	Pancreatic	1625:1634	arg1	tumors					1636:1641	Pancreatic tumors	1625:1641	Pancreatic tumors that developed in KPCC mice	1625:1669	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	0	44	theme	C1galt1	14:20	arg1	Gene					22:25	C1galt1 Gene	14:25	C1galt1 Gene	14:25	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	1	45	theme	&	113:113	arg1	AIMS					115:118	BACKGROUND & AIMS	102:118	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.	102:259	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	12	46	theme	aberrant	1974:1981	arg1	glycosylation					1983:1995	aberrant glycosylation	1974:1995	aberrant glycosylation of MUC16	1974:2004	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	6	47	from	C1GALT1	980:986	arg1	T3M4					1009:1012	T3M4	1009:1012	T3M4	1009:1012	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	47	from	C1GALT1	980:986	arg1	cells					1002:1006	human PDAC cells	991:1006	human PDAC cells (T3M4 and CD18/HPAF)	991:1027	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	47	from	C1GALT1	980:986	arg1	CD18/HPAF					1018:1026	CD18/HPAF	1018:1026	CD18/HPAF	1018:1026	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	10	48	theme	stroma	1773:1778	arg1	stroma					1773:1778	stroma	1773:1778	stroma	1773:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	48	theme	stroma	1773:1778	arg1	amount					1763:1768	a decreased amount	1751:1768	a decreased amount of stroma	1751:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	11	49	theme	differentiated	1824:1837	arg1	specimens					1844:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	7	50	theme	Orthotopic	1133:1142	arg1	studies					1144:1150	Orthotopic studies	1133:1150	Orthotopic studies	1133:1150	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	13	51	theme	KPC	2138:2140	arg1	mice					2142:2145	KPC mice	2138:2145	KPC mice with disruption of C1galt1	2138:2172	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	4	52	dep	Pdx1-Cre	670:677	arg1	KPC					680:682	KPC	680:682	KPC	680:682	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	7	53	theme	knockout	1220:1227	arg1	cells					1234:1238	knockout PDAC cells	1220:1238	knockout PDAC cells	1220:1238	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	10	54	theme	increased	1789:1797	arg1	production					1799:1808	increased production	1789:1808	increased production of Tn	1789:1814	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	11	55	theme	PDAC	1839:1842	arg1	specimens					1844:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	2	56	theme	increased	384:392	arg1	expression					394:403	increased expression	384:403	increased expression of these truncated O-glycans	384:432	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	9	57	theme	KPCC	1386:1389	arg1	mice					1391:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	8	58	dep	well-differentiated	1276:1294	arg1	n =					1297:1299	n = 36	1297:1302	n = 36	1297:1302	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	14	59	theme	O-glycosylation	2344:2358	arg1	truncation					2330:2339	truncation	2330:2339	truncation	2330:2339	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	14	59	theme	O-glycosylation	2344:2358	arg1	tumorigenicity					2311:2324	increased tumorigenicity	2301:2324	increased tumorigenicity	2301:2324	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	3	60	theme	PDAC	537:540	arg1	progression					522:532	progression	522:532	progression	522:532	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	3	60	theme	PDAC	537:540	arg1	development					506:516	development	506:516	development	506:516	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	6	61	theme	lectin	1070:1075	arg1	blotting					1077:1084	lectin blotting	1070:1084	lectin blotting	1070:1084	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	4	62	theme	floxed	608:613	arg1	C1galt1loxP/loxP					621:636	C1galt1loxP/loxP	621:636	C1galt1loxP/loxP	621:636	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	4	62	theme	floxed	608:613	arg1	mice					615:618	C1galt1 floxed mice	600:618	C1galt1 floxed mice (C1galt1loxP/loxP)	600:637	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	3	63	with	mice	548:551	arg1	disruption					558:567	disruption	558:567	disruption of C1galt1	558:578	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	3	64	theme	C1galt1	572:578	arg1	disruption					558:567	disruption	558:567	disruption of C1galt1	558:578	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	1	65	theme	normal	243:248	arg1	pancreata					250:258	normal pancreata	243:258	normal pancreata	243:258	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	14	66	from	tumorigenicity	2311:2324	arg1	MUC16					2363:2367	MUC16	2363:2367	MUC16	2363:2367	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	2	67	theme	Dysregulated	261:272	arg1	activity					274:281	Dysregulated activity	261:281	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1)	261:373	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	1	68	theme	higher	170:175	arg1	levels					177:182	higher levels	170:182	higher levels of truncated O-glycan structures (such as Tn and sTn)	170:236	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	12	69	theme	PDAC	1934:1937	arg1	cells					1939:1943	Human PDAC cells	1928:1943	Human PDAC cells with knockout of C1GALT1	1928:1968	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	14	70	from	truncation	2330:2339	arg1	MUC16					2363:2367	MUC16	2363:2367	MUC16	2363:2367	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	5	71	theme	KPCC	785:788	arg1	mice					790:793	KPCC mice	785:793	KPCC mice	785:793	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	2	72	theme	glycoprotein-N-acetylgalactosamine	302:335	arg1	3-β-galactosyltransferase					337:361	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1	286:363	core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1)	286:373	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	2	72	theme	glycoprotein-N-acetylgalactosamine	302:335	arg1	C1GALT1					366:372	C1GALT1	366:372	C1GALT1	366:372	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	9	73	theme	median	1438:1443	arg1	times					1431:1435	significantly shorter survival times	1400:1435	significantly shorter survival times (median 102 days)	1400:1453	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	73	theme	median	1438:1443	arg1	days					1449:1452	median 102 days	1438:1452	median 102 days	1438:1452	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	74	contain	had	1396:1398	arg2	times					1431:1435	significantly shorter survival times	1400:1435	significantly shorter survival times (median 102 days)	1400:1453	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	74	contain	had	1396:1398	arg1	mice					1391:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	74	contain	had	1396:1398	arg2	days					1449:1452	median 102 days	1438:1452	median 102 days	1438:1452	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	1	75	theme	O-glycan	197:204	arg1	structures					206:215	truncated O-glycan structures	187:215	truncated O-glycan structures (such as Tn and sTn)	187:236	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	6	76	theme	pulldown	1117:1124	arg1	assay					1126:1130	lectin pulldown assay	1110:1130	lectin pulldown assay	1110:1130	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	5	77	theme	red	899:901	arg1	Sirius					892:897	Sirius red	892:901	Sirius red	892:901	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	6	78	theme	CRISPR/Cas9	950:960	arg1	system					962:967	the CRISPR/Cas9 system	946:967	the CRISPR/Cas9 system	946:967	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	13	79	dep	CONCLUSIONS	2112:2122	arg1	studies					2127:2133	studies	2127:2133	studies of KPC mice with disruption of C1galt1	2127:2172	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	4	80	theme	Pdx1-Cre	670:677	arg1	mice					685:688	Pdx1-Cre (KPC) mice	670:688	KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice	644:688	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	13	81	theme	C1galt1	2197:2203	arg1	loss					2189:2192	loss	2189:2192	loss of C1galt1	2189:2203	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	12	82	theme	MUC16	2000:2004	arg1	glycosylation					1983:1995	aberrant glycosylation	1974:1995	aberrant glycosylation of MUC16	1974:2004	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	8	83	theme	well-differentiated	1276:1294	arg1	samples					1345:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	13	84	theme	increased	2250:2258	arg1	metastasis					2260:2269	increased metastasis	2250:2269	increased metastasis	2250:2269	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	14	85	theme	C1galt1	2284:2290	arg1	Knockout					2272:2279	Knockout	2272:2279	Knockout	2272:2279	Knockout of C1galt1 leads to increased tumorigenicity and truncation of O-glycosylation on MUC16, which could contribute to increased aggressiveness.					
30086262	4	86	dep	KrasG12D/+	644:653	arg1	Trp53R172H/+					656:667	Trp53R172H/+	656:667	Trp53R172H/+	656:667	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	4	86	dep	KrasG12D/+	644:653	arg1	mice					685:688	Pdx1-Cre (KPC) mice	670:688	KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice	644:688	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	10	87	theme	decreased	1753:1761	arg1	stroma					1773:1778	stroma	1773:1778	stroma	1773:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	87	theme	decreased	1753:1761	arg1	amount					1763:1768	a decreased amount	1751:1768	a decreased amount of stroma	1751:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	1	88	theme	ductal	131:136	arg1	PDACs					155:159	PDACs	155:159	PDACs	155:159	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	1	88	theme	ductal	131:136	arg1	adenocarcinomas					138:152	Pancreatic ductal adenocarcinomas	120:152	Pancreatic ductal adenocarcinomas (PDACs)	120:160	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	3	89	dep	development	506:516	arg1	the					502:504	the	502:504	the	502:504	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	0	90	theme	Pancreatic	66:75	arg1	Adenocarcinomas					77:91	Pancreatic Adenocarcinomas	66:91	Pancreatic Adenocarcinomas	66:91	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	11	91	theme	C1GALT1	1888:1894	arg1	levels					1878:1883	significantly lower levels	1858:1883	significantly lower levels of C1GALT1	1858:1894	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	13	92	theme	aggressive	2229:2238	arg1	PDACs					2240:2244	aggressive PDACs	2229:2244	aggressive PDACs	2229:2244	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	10	93	dep	aggressive	1681:1690	arg1	metastases					1711:1720	metastases	1711:1720	metastases	1711:1720	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	93	dep	aggressive	1681:1690	arg1	invasive					1698:1705	invasive	1698:1705	invasive	1698:1705	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	6	94	dep	cells	1002:1006	arg1	T3M4					1009:1012	T3M4	1009:1012	T3M4	1009:1012	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	94	dep	cells	1002:1006	arg1	cells					1002:1006	human PDAC cells	991:1006	human PDAC cells (T3M4 and CD18/HPAF)	991:1027	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	94	dep	cells	1002:1006	arg1	CD18/HPAF					1018:1026	CD18/HPAF	1018:1026	CD18/HPAF	1018:1026	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	5	95	theme	pancreatic	737:746	arg1	tumors					748:753	pancreatic tumors	737:753	pancreatic tumors	737:753	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	11	96	contain	had	1854:1856	arg1	specimens					1844:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens	1817:1852	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	11	96	contain	had	1854:1856	arg2	levels					1878:1883	significantly lower levels	1858:1883	significantly lower levels of C1GALT1	1858:1894	Poorly differentiated PDAC specimens had significantly lower levels of C1GALT1 than well-differentiated PDACs.					
30086262	9	97	theme	early	1501:1505	arg1	neoplasias					1534:1543	early pancreatic intraepithelial neoplasias	1501:1543	early pancreatic intraepithelial neoplasias at 3 weeks	1501:1554	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	6	98	theme	human	991:995	arg1	T3M4					1009:1012	T3M4	1009:1012	T3M4	1009:1012	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	98	theme	human	991:995	arg1	cells					1002:1006	human PDAC cells	991:1006	human PDAC cells (T3M4 and CD18/HPAF)	991:1027	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	6	98	theme	human	991:995	arg1	CD18/HPAF					1018:1026	CD18/HPAF	1018:1026	CD18/HPAF	1018:1026	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	0	99	from	Metastasis	52:61	arg1	Mice					96:99	Mice	96:99	Mice	96:99	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	13	100	theme	PDACs	2240:2244	arg1	development					2214:2224	development	2214:2224	development of aggressive PDACs	2214:2244	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	13	100	theme	PDACs	2240:2244	arg1	metastasis					2260:2269	increased metastasis	2250:2269	increased metastasis	2250:2269	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	9	101	theme	intraepithelial	1518:1532	arg1	neoplasias					1534:1543	early pancreatic intraepithelial neoplasias	1501:1543	early pancreatic intraepithelial neoplasias at 3 weeks	1501:1554	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	0	102	from	Development	36:46	arg1	Mice					96:99	Mice	96:99	Mice	96:99	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	3	103	theme	truncated	467:475	arg1	O-glycans					477:485	truncated O-glycans	467:485	truncated O-glycans	467:485	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	7	104	theme	RNA	1156:1158	arg1	analyses					1171:1178	RNA sequencing analyses	1156:1178	RNA sequencing analyses	1156:1178	Orthotopic studies and RNA sequencing analyses were performed with control and C1GALT1 knockout PDAC cells.					
30086262	4	105	dep	METHODS	581:587	arg1	We					589:590	We	589:590	We	589:590	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	3	106	dep	investigated	438:449	arg1	whether					451:457	whether	451:457	whether	451:457	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	8	107	theme	PDAC	1340:1343	arg1	samples					1345:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples	1276:1351	C1GALT1 expression was analyzed in well-differentiated (n = 36) and poorly differentiated (n = 23) PDAC samples by immunohistochemistry.					
30086262	0	108	theme	Gene	22:25	arg1	Disruption					0:9	Disruption	0:9	Disruption of C1galt1 Gene	0:25	Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice.					
30086262	13	109	theme	C1galt1	2166:2172	arg1	disruption					2152:2161	disruption	2152:2161	disruption of C1galt1	2152:2172	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	1	110	theme	BACKGROUND	102:111	arg1	AIMS					115:118	BACKGROUND & AIMS	102:118	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.	102:259	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
30086262	9	111	from	weeks	1567:1571	arg1	PDAC					1557:1560	PDAC	1557:1560	PDAC at 5 weeks	1557:1571	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	111	from	weeks	1567:1571	arg1	neoplasias					1534:1543	early pancreatic intraepithelial neoplasias	1501:1543	early pancreatic intraepithelial neoplasias at 3 weeks	1501:1554	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	9	111	from	weeks	1567:1571	arg1	metastasis					1578:1587	metastasis	1578:1587	metastasis	1578:1587	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	4	112	theme	C1galt1	600:606	arg1	C1galt1loxP/loxP					621:636	C1galt1loxP/loxP	621:636	C1galt1loxP/loxP	621:636	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	4	112	theme	C1galt1	600:606	arg1	mice					615:618	C1galt1 floxed mice	600:618	C1galt1 floxed mice (C1galt1loxP/loxP)	600:637	METHODS We crossed C1galt1 floxed mice (C1galt1loxP/loxP) with KrasG12D/+; Trp53R172H/+; Pdx1-Cre (KPC) mice to create KPCC mice.					
30086262	3	113	dep	whether	451:457	arg1	contributes					487:497	contributes	487:497	contributes to the development and progression of PDAC using mice with disruption of C1galt1	487:578	We investigated whether and how truncated O-glycans contributes to the development and progression of PDAC using mice with disruption of C1galt1.					
30086262	10	114	contain	had	1747:1749	arg2	amount					1763:1768	a decreased amount	1751:1768	a decreased amount of stroma	1751:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	114	contain	had	1747:1749	arg1	tumors					1636:1641	Pancreatic tumors	1625:1641	Pancreatic tumors that developed in KPCC mice	1625:1669	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	10	114	contain	had	1747:1749	arg2	stroma					1773:1778	stroma	1773:1778	stroma	1773:1778	Pancreatic tumors that developed in KPCC mice were more aggressive (more invasive and metastases) than those in KPC mice, had a decreased amount of stroma, and had increased production of Tn.					
30086262	2	115	theme	O-glycans	424:432	arg1	expression					394:403	increased expression	384:403	increased expression of these truncated O-glycans	384:432	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	5	116	theme	pancreatic	796:805	arg1	tissues					807:813	pancreatic tissues	796:813	pancreatic tissues	796:813	Growth and progression of pancreatic tumors were compared between KPC and KPCC mice; pancreatic tissues were collected and analyzed by immunohistochemistry; immunofluorescence; and Sirius red, alcian blue, and lectin staining.					
30086262	13	117	theme	mice	2142:2145	arg1	studies					2127:2133	studies	2127:2133	studies of KPC mice with disruption of C1galt1	2127:2172	CONCLUSIONS In studies of KPC mice with disruption of C1galt1, we found that loss of C1galt1 promotes development of aggressive PDACs and increased metastasis.					
30086262	6	118	theme	lectin	1110:1115	arg1	assay					1126:1130	lectin pulldown assay	1110:1130	lectin pulldown assay	1110:1130	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	2	119	theme	3-β-galactosyltransferase	337:361	arg1	activity					274:281	Dysregulated activity	261:281	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1)	261:373	Dysregulated activity of core 1 synthase glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1 (C1GALT1) leads to increased expression of these truncated O-glycans.					
30086262	9	120	theme	RESULTS	1378:1384	arg1	mice					1391:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice	1378:1394	RESULTS KPCC mice had significantly shorter survival times (median 102 days) than KPC mice (median 200 days) and developed early pancreatic intraepithelial neoplasias at 3 weeks, PDAC at 5 weeks, and metastasis at 10 weeks compared with KPC mice.					
30086262	12	121	theme	increased	2038:2046	arg1	expression					2048:2057	increased expression	2038:2057	increased expression of genes that regulate tumorigenesis and metastasis	2038:2109	Human PDAC cells with knockout of C1GALT1 had aberrant glycosylation of MUC16 compared with control cells and increased expression of genes that regulate tumorigenesis and metastasis.					
30086262	6	122	theme	mass	1087:1090	arg1	spectrometry					1092:1103	mass spectrometry	1087:1103	mass spectrometry	1087:1103	We used the CRISPR/Cas9 system to disrupt C1GALT1 in human PDAC cells (T3M4 and CD18/HPAF) and levels of O-glycans were analyzed by lectin blotting, mass spectrometry, and lectin pulldown assay.					
30086262	1	123	dep	structures	206:215	arg1	such					218:221	such	218:221	such	218:221	BACKGROUND & AIMS Pancreatic ductal adenocarcinomas (PDACs) produce higher levels of truncated O-glycan structures (such as Tn and sTn) than normal pancreata.					
31987138	7	0	theme	low	1200:1202	arg1	levels					1209:1214	low fmol levels	1200:1214	low fmol levels	1200:1214	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	0	theme	low	1200:1202	arg1	sensitivity					1187:1197	sensitivity	1187:1197	sensitivity (low fmol levels)	1187:1215	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	3	1	theme	current	425:431	arg1	reactions					451:459	current covalent coupling reactions	425:459	current covalent coupling reactions for N-glycopeptides enrichment	425:490	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	3	2	theme	coupling	518:525	arg1	time					527:530	long coupling time	513:530	long coupling time	513:530	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	4	3	theme	formation-based	599:613	arg1	approach					615:622	a thiazolidine formation-based approach	584:622	a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	584:699	Herein, we developed a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	7	4	theme	non-glycopeptides	1296:1312	arg1	mixture					1267:1273	the mixture	1263:1273	the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio	1263:1335	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	5	theme	fmol	1204:1207	arg1	levels					1209:1214	low fmol levels	1200:1214	low fmol levels	1200:1214	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	5	theme	fmol	1204:1207	arg1	sensitivity					1187:1197	sensitivity	1187:1197	sensitivity (low fmol levels)	1187:1215	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	1	6	theme	selective	170:178	arg1	step					244:247	a crucial step	234:247	a crucial step to reduce sample complexity	234:275	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	6	theme	selective	170:178	arg1	enrichment					180:189	selective enrichment	170:189	selective enrichment of N-glycopeptides prior to MS analysis	170:229	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	7	theme	sample	259:264	arg1	complexity					266:275	sample complexity	259:275	sample complexity	259:275	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	7	8	gly	N-glycopeptides	1242:1256	arg2	N-glycopeptides					1242:1256	N-glycopeptides	1242:1256	N-glycopeptides	1242:1256	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	9	theme	sensitivity	1187:1197	arg1	terms					1178:1182	terms	1178:1182	terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%)	1178:1366	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	10	from	ratio	1331:1335	arg1	mixture					1267:1273	the mixture	1263:1273	the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio	1263:1335	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	2	11	theme	covalent	298:305	arg1	coupling					307:314	covalent coupling	298:314	covalent coupling	298:314	Enrichment based on covalent coupling is as an increasingly attractive strategy due to the unbiased and highly specific features.					
31987138	6	12	theme	catalyst	1056:1063	arg1	addition					1038:1045	the addition	1034:1045	the addition of toxic catalyst or sample-destroying reducing agent	1034:1099	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	0	13	theme	N-Glycoproteome	101:115	arg1	extraction					87:96	ultrafast and highly efficient solid-phase extraction	44:96	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	44:115	A thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	5	14	from	groups	875:880	arg1	surface					889:895	the surface	885:895	the surface of magnetic nanoparticles through thiazolidine formation	885:952	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	1	15	gly	N-glycopeptides	194:208	arg2	N-glycopeptides					194:208	N-glycopeptides	194:208	N-glycopeptides	194:208	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	7	16	theme	1:100 molar	1319:1329	arg1	ratio					1331:1335	a 1:100 molar ratio	1317:1335	a 1:100 molar ratio	1317:1335	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	6	17	theme	toxic	1050:1054	arg1	catalyst					1056:1063	toxic catalyst	1050:1063	toxic catalyst	1050:1063	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	5	18	theme	glycan	790:795	arg1	moieties					797:804	the oxidized glycan moieties	777:804	the oxidized glycan moieties on glycopeptides	777:821	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	2	19	theme	due	358:360	arg1	strategy					349:356	an increasingly attractive strategy	322:356	an increasingly attractive strategy due to the unbiased and highly specific features	322:405	Enrichment based on covalent coupling is as an increasingly attractive strategy due to the unbiased and highly specific features.					
31987138	4	20	theme	solid-phase	659:669	arg1	extraction					671:680	ultrafast and highly efficient solid-phase extraction	628:680	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	628:699	Herein, we developed a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	3	21	theme	coupling	542:549	arg1	conditions					551:560	harsh coupling conditions	536:560	harsh coupling conditions	536:560	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	1	22	theme	N-glycopeptides	194:208	arg1	step					244:247	a crucial step	234:247	a crucial step to reduce sample complexity	234:275	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	22	theme	N-glycopeptides	194:208	arg1	enrichment					180:189	selective enrichment	170:189	selective enrichment of N-glycopeptides prior to MS analysis	170:229	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	3	23	theme	coupling	442:449	arg1	reactions					451:459	current covalent coupling reactions	425:459	current covalent coupling reactions for N-glycopeptides enrichment	425:490	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	8	24	theme	complex	1570:1576	arg1	samples					1589:1595	complex biological samples	1570:1595	complex biological samples	1570:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	6	25	theme	sample-destroying	1068:1084	arg1	agent					1095:1099	sample-destroying reducing agent	1068:1099	sample-destroying reducing agent	1068:1099	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	4	26	theme	ultrafast	628:636	arg1	extraction					671:680	ultrafast and highly efficient solid-phase extraction	628:680	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	628:699	Herein, we developed a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	8	27	from	serum	1478:1482	arg1	N-glycoproteome					1446:1460	the N-glycoproteome	1442:1460	the N-glycoproteome	1442:1460	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	5	28	theme	β-amino	860:866	arg1	groups					875:880	the β-amino thiols groups	856:880	the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation	856:952	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	7	29	theme	enrichment	1118:1127	arg1	performance					1129:1139	the great enrichment performance	1108:1139	the great enrichment performance for N-glycopeptides	1108:1159	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	2	30	theme	specific	389:396	arg1	features					398:405	the unbiased and highly specific features	365:405	the unbiased and highly specific features	365:405	Enrichment based on covalent coupling is as an increasingly attractive strategy due to the unbiased and highly specific features.					
31987138	5	31	theme	thiols	868:873	arg1	groups					875:880	the β-amino thiols groups	856:880	the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation	856:952	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	7	32	theme	selectivity	1218:1228	arg1	terms					1178:1182	terms	1178:1182	terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%)	1178:1366	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	33	dep	selectivity	1218:1228	arg1	extracting					1231:1240	extracting	1231:1240	extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio	1231:1335	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	3	34	theme	covalent	433:440	arg1	reactions					451:459	current covalent coupling reactions	425:459	current covalent coupling reactions for N-glycopeptides enrichment	425:490	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	5	35	theme	Cys-terminated	738:751	arg1	nanoparticles					762:774	Cys-terminated magnetic nanoparticles	738:774	Cys-terminated magnetic nanoparticles	738:774	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	7	36	gly	non-glycopeptides	1296:1312	arg2	non-glycopeptides					1296:1312	non-glycopeptides	1296:1312	non-glycopeptides	1296:1312	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	7	37	gly	glycopeptides	1278:1290	arg2	glycopeptides					1278:1290	glycopeptides	1278:1290	glycopeptides	1278:1290	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	8	38	from	samples	1589:1595	arg1	analysis					1537:1544	analysis	1537:1544	analysis of N-glycoproteome from complex biological samples	1537:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	8	38	from	samples	1589:1595	arg1	N-glycoproteome					1549:1563	N-glycoproteome	1549:1563	N-glycoproteome from complex biological samples	1549:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	5	39	theme	magnetic	753:760	arg1	nanoparticles					762:774	Cys-terminated magnetic nanoparticles	738:774	Cys-terminated magnetic nanoparticles	738:774	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	1	40	theme	mass	122:125	arg1	MS					141:142	MS	141:142	MS	141:142	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	40	theme	mass	122:125	arg1	spectrometry					127:138	mass spectrometry	122:138	mass spectrometry (MS)-based N-glycoproteomics	122:167	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	41	theme	MS	219:220	arg1	analysis					222:229	MS analysis	219:229	MS analysis	219:229	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	0	42	theme	ultrafast	44:52	arg1	extraction					87:96	ultrafast and highly efficient solid-phase extraction	44:96	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	44:115	A thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	5	43	theme	nanoparticles	762:774	arg1	synthesis					725:733	facile synthesis	718:733	facile synthesis of Cys-terminated magnetic nanoparticles	718:774	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	5	44	from	moieties	797:804	arg1	glycopeptides					809:821	glycopeptides	809:821	glycopeptides	809:821	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	8	45	theme	proposed	1383:1390	arg1	method					1392:1397	this proposed method	1378:1397	this proposed method	1378:1397	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	4	46	theme	N-Glycoproteome	685:699	arg1	extraction					671:680	ultrafast and highly efficient solid-phase extraction	628:680	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	628:699	Herein, we developed a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	5	47	theme	nanoparticles	909:921	arg1	surface					889:895	the surface	885:895	the surface of magnetic nanoparticles through thiazolidine formation	885:952	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	5	48	theme	thiazolidine	931:942	arg1	formation					944:952	thiazolidine formation	931:952	thiazolidine formation	931:952	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	6	49	theme	agent	1095:1099	arg1	addition					1038:1045	the addition	1034:1045	the addition of toxic catalyst or sample-destroying reducing agent	1034:1099	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	3	50	theme	N-glycopeptides	465:479	arg1	enrichment					481:490	N-glycopeptides enrichment	465:490	N-glycopeptides enrichment	465:490	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	7	51	theme	great	1112:1116	arg1	performance					1129:1139	the great enrichment performance	1108:1139	the great enrichment performance for N-glycopeptides	1108:1159	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	8	52	from	analysis	1537:1544	arg1	samples					1589:1595	complex biological samples	1570:1595	complex biological samples	1570:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	3	53	gly	N-glycopeptides	465:479	arg2	N-glycopeptides					465:479	N-glycopeptides enrichment	465:490	N-glycopeptides enrichment	465:490	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	1	54	theme	spectrometry	127:138	arg1	N-glycoproteomics					151:167	mass spectrometry (MS)-based N-glycoproteomics	122:167	mass spectrometry (MS)-based N-glycoproteomics	122:167	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	6	55	theme	mild	1006:1009	arg1	condition					1011:1019	mild condition	1006:1019	mild condition	1006:1019	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	5	56	theme	facile	718:723	arg1	synthesis					725:733	facile synthesis	718:733	facile synthesis of Cys-terminated magnetic nanoparticles	718:774	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	7	57	theme	glycopeptides	1278:1290	arg1	mixture					1267:1273	the mixture	1263:1273	the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio	1263:1335	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	3	58	theme	long	513:516	arg1	time					527:530	long coupling time	513:530	long coupling time	513:530	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	8	59	theme	alternative	1501:1511	arg1	method					1526:1531	an alternative purification method	1498:1531	an alternative purification method for analysis of N-glycoproteome from complex biological samples	1498:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	5	60	theme	synthesis	725:733	arg1	use					711:713	the use	707:713	the use of facile synthesis of Cys-terminated magnetic nanoparticles	707:774	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	8	61	theme	N-glycoproteome	1549:1563	arg1	analysis					1537:1544	analysis	1537:1544	analysis of N-glycoproteome from complex biological samples	1537:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	8	62	theme	human	1472:1476	arg1	serum					1478:1482	2 μL human serum	1467:1482	2 μL human serum	1467:1482	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	2	63	theme	attractive	338:347	arg1	strategy					349:356	an increasingly attractive strategy	322:356	an increasingly attractive strategy due to the unbiased and highly specific features	322:405	Enrichment based on covalent coupling is as an increasingly attractive strategy due to the unbiased and highly specific features.					
31987138	8	64	theme	2 μL	1467:1470	arg1	serum					1478:1482	2 μL human serum	1467:1482	2 μL human serum	1467:1482	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	7	65	gly	N-glycopeptides	1145:1159	arg2	N-glycopeptides					1145:1159	N-glycopeptides	1145:1159	N-glycopeptides	1145:1159	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	8	66	theme	biological	1578:1587	arg1	samples					1589:1595	complex biological samples	1570:1595	complex biological samples	1570:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	5	67	gly	glycopeptides	809:821	arg2	glycopeptides					809:821	glycopeptides	809:821	glycopeptides	809:821	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	3	68	theme	harsh	536:540	arg1	conditions					551:560	harsh coupling conditions	536:560	harsh coupling conditions	536:560	However, most of current covalent coupling reactions for N-glycopeptides enrichment are still limited by long coupling time and harsh coupling conditions.					
31987138	1	69	theme	crucial	236:242	arg1	step					244:247	a crucial step	234:247	a crucial step to reduce sample complexity	234:275	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	1	69	theme	crucial	236:242	arg1	enrichment					180:189	selective enrichment	170:189	selective enrichment of N-glycopeptides prior to MS analysis	170:229	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	0	70	theme	solid-phase	75:85	arg1	extraction					87:96	ultrafast and highly efficient solid-phase extraction	44:96	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	44:115	A thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	5	71	theme	magnetic	900:907	arg1	nanoparticles					909:921	magnetic nanoparticles	900:921	magnetic nanoparticles	900:921	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
31987138	8	72	theme	purification	1513:1524	arg1	method					1526:1531	an alternative purification method	1498:1531	an alternative purification method for analysis of N-glycoproteome from complex biological samples	1498:1595	Finally, this proposed method was successfully demonstrated by analyzing the N-glycoproteome from 2 μL human serum, which offers an alternative purification method for analysis of N-glycoproteome from complex biological samples.					
31987138	1	73	theme	-based	144:149	arg1	N-glycoproteomics					151:167	mass spectrometry (MS)-based N-glycoproteomics	122:167	mass spectrometry (MS)-based N-glycoproteomics	122:167	For mass spectrometry (MS)-based N-glycoproteomics, selective enrichment of N-glycopeptides prior to MS analysis is a crucial step to reduce sample complexity.					
31987138	4	74	theme	efficient	649:657	arg1	extraction					671:680	ultrafast and highly efficient solid-phase extraction	628:680	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	628:699	Herein, we developed a thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	0	75	theme	efficient	65:73	arg1	extraction					87:96	ultrafast and highly efficient solid-phase extraction	44:96	ultrafast and highly efficient solid-phase extraction of N-Glycoproteome	44:115	A thiazolidine formation-based approach for ultrafast and highly efficient solid-phase extraction of N-Glycoproteome.					
31987138	6	76	theme	reducing	1086:1093	arg1	agent					1095:1099	sample-destroying reducing agent	1068:1099	sample-destroying reducing agent	1068:1099	The coupling could be achieved within 30 min under mild condition, eliminating the addition of toxic catalyst or sample-destroying reducing agent.					
31987138	7	77	theme	reproducibility	1342:1356	arg1	terms					1178:1182	terms	1178:1182	terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%)	1178:1366	Also, the great enrichment performance for N-glycopeptides were obtained in terms of sensitivity (low fmol levels), selectivity (extracting N-glycopeptides from the mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio) and reproducibility (CVs<26%).					
31987138	2	78	theme	unbiased	369:376	arg1	features					398:405	the unbiased and highly specific features	365:405	the unbiased and highly specific features	365:405	Enrichment based on covalent coupling is as an increasingly attractive strategy due to the unbiased and highly specific features.					
31987138	5	79	theme	oxidized	781:788	arg1	moieties					797:804	the oxidized glycan moieties	777:804	the oxidized glycan moieties on glycopeptides	777:821	With the use of facile synthesis of Cys-terminated magnetic nanoparticles, the oxidized glycan moieties on glycopeptides could be selectively captured by the β-amino thiols groups on the surface of magnetic nanoparticles through thiazolidine formation.					
30283149	8	0	theme	particle	1206:1213	arg1	structure					1215:1223	the particle structure	1202:1223	the particle structure	1202:1223	We demonstrated that the particle-protein interaction could be modulated by the particle structure and composition.					
30283149	1	1	theme	macromolecular	180:193	arg1	design					195:200	macromolecular design	180:200	macromolecular design	180:200	Polysaccharides represent a versatile class of building blocks that are used in macromolecular design.					
30283149	9	2	theme	drug	1496:1499	arg1	delivery					1501:1508	drug delivery	1496:1508	drug delivery	1496:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	5	3	theme	copolymers	811:820	arg1	co-nanoprecipitation					779:798	co-nanoprecipitation	779:798	co-nanoprecipitation of the two copolymers	779:820	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	9	4	theme	versatile	1331:1339	arg1	method					1278:1283	this method	1273:1283	this method based on nanoprecipitation	1273:1310	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	9	4	theme	versatile	1331:1339	arg1	way					1341:1343	a practical and versatile way	1315:1343	a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery	1315:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	2	5	theme	nanostructures	391:404	arg1	level					330:334	the level	326:334	the level of the polymer chains and the resulting self-assembled nanostructures	326:404	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	5	6	theme	hybrid	698:703	arg1	particles					705:713	hybrid particles	698:713	hybrid particles containing both HA and LAM in different ratios	698:760	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	2	7	dep	level	330:334	arg1	both					318:321	both	318:321	both	318:321	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	5	8	from	LAM	738:740	arg1	ratios					755:760	different ratios	745:760	different ratios	745:760	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	9	9	theme	controllable	1370:1381	arg1	interactions					1383:1394	controllable interactions	1370:1394	controllable interactions	1370:1394	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	0	10	theme	Multivalent	0:10	arg1	particles					53:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition.					
30283149	2	11	theme	self-assembled	376:389	arg1	nanostructures					391:404	the resulting self-assembled nanostructures	362:404	the resulting self-assembled nanostructures	362:404	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	6	12	theme	self-assembly	842:854	arg1	process					856:862	the self-assembly process	838:862	the self-assembly process	838:862	By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed.					
30283149	3	13	theme	poly	495:498	arg1	PBLG					522:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	1	14	used	used	172:175	arg2	blocks					156:161	building blocks	147:161	building blocks that are used in macromolecular design	147:200	Polysaccharides represent a versatile class of building blocks that are used in macromolecular design.					
30283149	3	15	theme	amphiphilic	428:438	arg1	copolymers					448:457	amphiphilic diblock copolymers	428:457	amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM)	428:598	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	2	16	theme	chains	351:356	arg1	level					330:334	the level	326:334	the level of the polymer chains and the resulting self-assembled nanostructures	326:404	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	3	17	theme	diblock	440:446	arg1	copolymers					448:457	amphiphilic diblock copolymers	428:457	amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM)	428:598	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	6	18	theme	particle	870:877	arg1	samples					879:885	five particle samples	865:885	five particle samples with different morphologies and compositions	865:930	By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed.					
30283149	2	19	theme	polymer	343:349	arg1	chains					351:356	the polymer chains	339:356	the polymer chains	339:356	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	3	20	theme	helical	487:493	arg1	PBLG					522:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	0	21	theme	polysaccharide-based	32:51	arg1	particles					53:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition.					
30283149	7	22	theme	relevant	1003:1010	arg1	proteins					1012:1019	biologically relevant proteins	990:1019	biologically relevant proteins for HA and LAM	990:1034	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	7	22	theme	relevant	1003:1010	arg1	Dectin-1					1053:1060	Dectin-1	1053:1060	Dectin-1 respectively	1053:1073	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	7	22	theme	relevant	1003:1010	arg1	CD44					1044:1047	CD44	1044:1047	CD44	1044:1047	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	6	23	with	samples	879:885	arg1	compositions					919:930	compositions	919:930	compositions	919:930	By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed.					
30283149	6	23	with	samples	879:885	arg1	morphologies					902:913	different morphologies	892:913	different morphologies	892:913	By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed.					
30283149	1	24	theme	versatile	128:136	arg1	class					138:142	a versatile class	126:142	a versatile class of building blocks that are used in macromolecular design	126:200	Polysaccharides represent a versatile class of building blocks that are used in macromolecular design.					
30283149	3	25	theme	hyaluronic	559:568	arg1	acid					570:573	hyaluronic acid	559:573	hyaluronic acid (HA)	559:578	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	3	25	theme	hyaluronic	559:568	arg1	polysaccharides					535:549	two polysaccharides	531:549	two polysaccharides	531:549	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	3	25	theme	hyaluronic	559:568	arg1	HA					576:577	HA	576:577	HA	576:577	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	2	26	theme	resulting	366:374	arg1	nanostructures					391:404	the resulting self-assembled nanostructures	362:404	the resulting self-assembled nanostructures	362:404	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	5	27	theme	different	745:753	arg1	ratios					755:760	different ratios	745:760	different ratios	745:760	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	2	28	theme	biological	278:287	arg1	properties					289:298	various physico-chemical and biological properties	249:298	properties	289:298	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	3	29	theme	hydrophobic	471:481	arg1	PBLG					522:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG	469:525	Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM).					
30283149	9	30	theme	appropriate	1426:1436	arg1	properties					1449:1458	the appropriate biological properties	1422:1458	the appropriate biological properties for biomedical applications such as drug delivery	1422:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	0	31	theme	receptor	78:85	arg1	recognition					87:97	controlled receptor recognition	67:97	controlled receptor recognition	67:97	Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition.					
30283149	9	32	theme	biological	1438:1447	arg1	properties					1449:1458	the appropriate biological properties	1422:1458	the appropriate biological properties for biomedical applications such as drug delivery	1422:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	7	33	theme	plasmon	1101:1107	arg1	SPR					1120:1122	SPR	1120:1122	SPR	1120:1122	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	7	33	theme	plasmon	1101:1107	arg1	resonance					1109:1117	surface plasmon resonance	1093:1117	surface plasmon resonance (SPR)	1093:1123	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	1	34	theme	building	147:154	arg1	blocks					156:161	building blocks	147:161	building blocks that are used in macromolecular design	147:200	Polysaccharides represent a versatile class of building blocks that are used in macromolecular design.					
30283149	0	35	theme	controlled	67:76	arg1	recognition					87:97	controlled receptor recognition	67:97	controlled receptor recognition	67:97	Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition.					
30283149	9	36	theme	practical	1317:1325	arg1	method					1278:1283	this method	1273:1283	this method based on nanoprecipitation	1273:1310	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	9	36	theme	practical	1317:1325	arg1	way					1341:1343	a practical and versatile way	1315:1343	a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery	1315:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	8	37	theme	particle-protein	1151:1166	arg1	interaction					1168:1178	the particle-protein interaction	1147:1178	the particle-protein interaction	1147:1178	We demonstrated that the particle-protein interaction could be modulated by the particle structure and composition.					
30283149	6	38	theme	different	892:900	arg1	morphologies					902:913	different morphologies	892:913	different morphologies	892:913	By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed.					
30283149	1	39	theme	blocks	156:161	arg1	class					138:142	a versatile class	126:142	a versatile class of building blocks that are used in macromolecular design	126:200	Polysaccharides represent a versatile class of building blocks that are used in macromolecular design.					
30283149	5	40	contain	containing	715:724	arg2	HA					731:732	HA	731:732	HA	731:732	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	5	40	contain	containing	715:724	arg2	LAM					738:740	LAM	738:740	LAM	738:740	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	5	40	contain	containing	715:724	arg1	particles					705:713	hybrid particles	698:713	hybrid particles containing both HA and LAM in different ratios	698:760	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	5	41	from	HA	731:732	arg1	ratios					755:760	different ratios	745:760	different ratios	745:760	In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers.					
30283149	7	42	theme	surface	1093:1099	arg1	SPR					1120:1122	SPR	1120:1122	SPR	1120:1122	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	7	42	theme	surface	1093:1099	arg1	resonance					1109:1117	surface plasmon resonance	1093:1117	surface plasmon resonance (SPR)	1093:1123	The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR).					
30283149	2	43	theme	saccharide	231:240	arg1	block					242:246	the appropriate saccharide block	215:246	the appropriate saccharide block	215:246	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	2	44	theme	appropriate	219:229	arg1	block					242:246	the appropriate saccharide block	215:246	the appropriate saccharide block	215:246	By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures.					
30283149	9	45	with	interactions	1383:1394	arg1	properties					1449:1458	the appropriate biological properties	1422:1458	the appropriate biological properties for biomedical applications such as drug delivery	1422:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	9	45	with	interactions	1383:1394	arg1	proteins					1401:1408	proteins	1401:1408	proteins	1401:1408	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	0	46	theme	multifunctional	16:30	arg1	particles					53:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles	0:61	Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition.					
30283149	9	47	theme	biomedical	1464:1473	arg1	delivery					1501:1508	drug delivery	1496:1508	drug delivery	1496:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30283149	9	47	theme	biomedical	1464:1473	arg1	applications					1475:1486	biomedical applications	1464:1486	biomedical applications such as drug delivery	1464:1508	It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.					
30412755	5	0	from	studies	889:895	arg1	composition					856:866	the optimum composition	844:866	the optimum composition from electrochemical studies	844:895	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	6	1	theme	ordinate	972:979	arg1	material					981:988	a super ordinate material	964:988	a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties	964:1064	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	3	2	dep	viability	627:635	arg1	study					637:641	study	637:641	study	637:641	Cytotoxicity of bio-nanocomposite has been inquired by cell viability study, which reveals its eco-friendly nature.					
30412755	6	3	theme	pure	995:998	arg1	PPy					1000:1002	pure PPy	995:1002	pure PPy	995:1002	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	5	4	theme	optimum	848:854	arg1	composition					856:866	the optimum composition	844:866	the optimum composition from electrochemical studies	844:895	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	1	5	theme	copolymer	347:355	arg1	HPG-g-PLLA					220:229	HPG-g-PLLA	220:229	HPG-g-PLLA	220:229	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	5	theme	copolymer	347:355	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	5	theme	copolymer	347:355	arg1	gum					215:217	hydroxypropyl guar gum	196:217	hydroxypropyl guar gum (HPG-g-PLLA)	196:230	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	5	theme	copolymer	347:355	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	5	theme	copolymer	347:355	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	7	6	theme	interfacial	1246:1256	arg1	complications					1258:1270	all the interfacial complications	1238:1270	all the interfacial complications arising from binders	1238:1291	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	6	7	theme	nanocomposite	911:923	arg1	copolymer					943:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer	898:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer	898:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	1	8	theme	poly	164:167	arg1	PLLA					182:185	PLLA	182:185	PLLA	182:185	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	8	theme	poly	164:167	arg1	acid					176:179	poly lactic acid	164:179	poly lactic acid (PLLA)	164:186	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	9	theme	polypyrrole/carboxylated	236:259	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	9	theme	polypyrrole/carboxylated	236:259	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	9	theme	polypyrrole/carboxylated	236:259	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	0	10	theme	electrochemical	100:114	arg1	efficacy					116:123	their electrochemical efficacy	94:123	their electrochemical efficacy	94:123	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	6	11	theme	hydrogel	902:909	arg1	copolymer					943:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer	898:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer	898:951	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	1	12	theme	multiwalled	261:271	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	12	theme	multiwalled	261:271	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	12	theme	multiwalled	261:271	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	5	13	theme	1 wt	819:822	arg1	C-MWCNT					825:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	1	14	theme	one	360:362	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	14	theme	one	360:362	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	15	theme	carbon	273:278	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	15	theme	carbon	273:278	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	15	theme	carbon	273:278	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	4	16	theme	voltammetric	764:775	arg1	studies					777:783	cyclic voltammetric studies	757:783	cyclic voltammetric studies	757:783	The electrochemical properties of the biomaterials have been appraised by cyclic voltammetric studies.					
30412755	7	17	theme	binder-free	1215:1225	arg1	nanocomposite					1155:1167	the hydrogel nanocomposite	1142:1167	the hydrogel nanocomposite	1142:1167	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	7	17	theme	binder-free	1215:1225	arg1	fabrication					1187:1197	making electrode fabrication	1170:1197	making electrode fabrication more simple and binder-free	1170:1225	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	3	18	theme	bio-nanocomposite	583:599	arg1	Cytotoxicity					567:578	Cytotoxicity	567:578	Cytotoxicity of bio-nanocomposite	567:599	Cytotoxicity of bio-nanocomposite has been inquired by cell viability study, which reveals its eco-friendly nature.					
30412755	1	19	theme	lactic	169:174	arg1	PLLA					182:185	PLLA	182:185	PLLA	182:185	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	19	theme	lactic	169:174	arg1	acid					176:179	poly lactic acid	164:179	poly lactic acid (PLLA)	164:186	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	0	20	theme	efficacy	116:123	arg1	study					85:89	study	85:89	study of their electrochemical efficacy	85:123	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	0	20	theme	efficacy	116:123	arg1	nanocomposite					67:79	hybrid hydrogel nanocomposite	51:79	hybrid hydrogel nanocomposite	51:79	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	0	20	theme	efficacy	116:123	arg1	efficacy					116:123	their electrochemical efficacy	94:123	their electrochemical efficacy	94:123	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	1	21	dep	one	360:362	arg1	synthesized					398:408	synthesized	398:408	have been synthesized successfully by in-situ polymerization	388:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	22	theme	nanocomposites	373:386	arg1	one					360:362	one	360:362	one	360:362	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	22	theme	nanocomposites	373:386	arg1	nanocomposites					373:386	these nanocomposites	367:386	these nanocomposites	367:386	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	7	23	theme	simple	1204:1209	arg1	nanocomposite					1155:1167	the hydrogel nanocomposite	1142:1167	the hydrogel nanocomposite	1142:1167	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	7	23	theme	simple	1204:1209	arg1	fabrication					1187:1197	making electrode fabrication	1170:1197	making electrode fabrication more simple and binder-free	1170:1225	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	2	24	theme	affable	470:476	arg1	nanocomposites					478:491	The environmentally affable nanocomposites	450:491	The environmentally affable nanocomposites	450:491	The environmentally affable nanocomposites have been characterized by spectroscopy, microscopy and thermogravimetry.					
30412755	1	25	theme	nanotube	280:287	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	25	theme	nanotube	280:287	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	25	theme	nanotube	280:287	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	2	26	theme	environmentally	454:468	arg1	nanocomposites					478:491	The environmentally affable nanocomposites	450:491	The environmentally affable nanocomposites	450:491	The environmentally affable nanocomposites have been characterized by spectroscopy, microscopy and thermogravimetry.					
30412755	6	27	theme	current	1071:1077	arg1	density					1079:1085	current density	1071:1085	current density	1071:1085	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	1	28	theme	PPy/C-MWCNT	290:300	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	28	theme	PPy/C-MWCNT	290:300	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	28	theme	PPy/C-MWCNT	290:300	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	6	29	theme	super	966:970	arg1	material					981:988	a super ordinate material	964:988	a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties	964:1064	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	3	30	theme	cell	622:625	arg1	viability					627:635	cell viability study	622:641	cell viability study	622:641	Cytotoxicity of bio-nanocomposite has been inquired by cell viability study, which reveals its eco-friendly nature.					
30412755	4	31	theme	electrochemical	687:701	arg1	properties					703:712	The electrochemical properties	683:712	The electrochemical properties of the biomaterials	683:732	The electrochemical properties of the biomaterials have been appraised by cyclic voltammetric studies.					
30412755	6	32	theme	properties	1055:1064	arg1	aspect					1029:1034	every aspect	1023:1034	every aspect of electrochemical properties	1023:1064	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	5	33	theme	PPy/C-MWCNT	790:800	arg1	C-MWCNT					825:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	1	34	theme	hydroxypropyl	196:208	arg1	HPG-g-PLLA					220:229	HPG-g-PLLA	220:229	HPG-g-PLLA	220:229	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	34	theme	hydroxypropyl	196:208	arg1	gum					215:217	hydroxypropyl guar gum	196:217	hydroxypropyl guar gum (HPG-g-PLLA)	196:230	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	0	35	theme	biopolymer	34:43	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	0	35	theme	biopolymer	34:43	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	6	36	theme	electrochemical	1039:1053	arg1	properties					1055:1064	electrochemical properties	1039:1064	electrochemical properties	1039:1064	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	5	37	theme	composite	802:810	arg1	C-MWCNT					825:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	1	38	theme	guar	210:213	arg1	HPG-g-PLLA					220:229	HPG-g-PLLA	220:229	HPG-g-PLLA	220:229	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	38	theme	guar	210:213	arg1	gum					215:217	hydroxypropyl guar gum	196:217	hydroxypropyl guar gum (HPG-g-PLLA)	196:230	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	0	39	theme	hydrogel	58:65	arg1	nanocomposite					67:79	hybrid hydrogel nanocomposite	51:79	hybrid hydrogel nanocomposite	51:79	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	0	39	theme	hydrogel	58:65	arg1	efficacy					116:123	their electrochemical efficacy	94:123	their electrochemical efficacy	94:123	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	5	40	theme	having	812:817	arg1	C-MWCNT					825:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	7	41	theme	electrode	1177:1185	arg1	nanocomposite					1155:1167	the hydrogel nanocomposite	1142:1167	the hydrogel nanocomposite	1142:1167	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	7	41	theme	electrode	1177:1185	arg1	fabrication					1187:1197	making electrode fabrication	1170:1197	making electrode fabrication more simple and binder-free	1170:1225	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	0	42	theme	hybrid	51:56	arg1	nanocomposite					67:79	hybrid hydrogel nanocomposite	51:79	hybrid hydrogel nanocomposite	51:79	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	0	42	theme	hybrid	51:56	arg1	efficacy					116:123	their electrochemical efficacy	94:123	their electrochemical efficacy	94:123	Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy.					
30412755	1	43	theme	various	316:322	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	44	theme	binary	324:329	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	45	theme	in-situ	426:432	arg1	polymerization					434:447	in-situ polymerization	426:447	in-situ polymerization	426:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	46	theme	competent	135:143	arg1	material					145:152	A highly competent material	126:152	A highly competent material	126:152	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	5	47	theme	%	823:823	arg1	C-MWCNT					825:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT	786:831	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	7	48	theme	hydrogel	1146:1153	arg1	nanocomposite					1155:1167	the hydrogel nanocomposite	1142:1167	the hydrogel nanocomposite	1142:1167	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	7	48	theme	hydrogel	1146:1153	arg1	fabrication					1187:1197	making electrode fabrication	1170:1197	making electrode fabrication more simple and binder-free	1170:1225	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	6	49	from	material	981:988	arg1	aspect					1029:1034	every aspect	1023:1034	every aspect of electrochemical properties	1023:1064	The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility.					
30412755	3	50	theme	eco-friendly	662:673	arg1	nature					675:680	its eco-friendly nature	658:680	its eco-friendly nature	658:680	Cytotoxicity of bio-nanocomposite has been inquired by cell viability study, which reveals its eco-friendly nature.					
30412755	4	51	theme	cyclic	757:762	arg1	studies					777:783	cyclic voltammetric studies	757:783	cyclic voltammetric studies	757:783	The electrochemical properties of the biomaterials have been appraised by cyclic voltammetric studies.					
30412755	4	52	theme	biomaterials	721:732	arg1	properties					703:712	The electrochemical properties	683:712	The electrochemical properties of the biomaterials	683:732	The electrochemical properties of the biomaterials have been appraised by cyclic voltammetric studies.					
30412755	5	53	theme	electrochemical	873:887	arg1	studies					889:895	electrochemical studies	873:895	electrochemical studies	873:895	The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies.					
30412755	1	54	theme	composition	331:341	arg1	HPG-g-PLLA					220:229	HPG-g-PLLA	220:229	HPG-g-PLLA	220:229	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	54	theme	composition	331:341	arg1	copolymer					347:355	copolymer	347:355	copolymer	347:355	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	54	theme	composition	331:341	arg1	gum					215:217	hydroxypropyl guar gum	196:217	hydroxypropyl guar gum (HPG-g-PLLA)	196:230	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	54	theme	composition	331:341	arg1	composition					331:341	various binary composition	316:341	various binary composition	316:341	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	1	54	theme	composition	331:341	arg1	composite					303:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite	236:311	polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization	236:447	A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization.					
30412755	7	55	theme	making	1170:1175	arg1	nanocomposite					1155:1167	the hydrogel nanocomposite	1142:1167	the hydrogel nanocomposite	1142:1167	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30412755	7	55	theme	making	1170:1175	arg1	fabrication					1187:1197	making electrode fabrication	1170:1197	making electrode fabrication more simple and binder-free	1170:1225	Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.					
30092985	0	0	theme	Anemarrhena	123:133	arg1	polysaccharides					155:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	1	1	theme	acids	391:395	arg1	detection					343:351	detection	343:351	detection	343:351	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	1	theme	acids	391:395	arg1	separation					328:337	simultaneous baseline separation	306:337	simultaneous baseline separation	306:337	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	7	2	from	analysis	1499:1506	arg1	medicines					1558:1566	traditional Chinese medicines	1538:1566	traditional Chinese medicines	1538:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	6	3	theme	polysaccharides	1220:1234	arg1	analysis					1191:1198	A comparative analysis	1177:1198	A comparative analysis of A. asphodeloides polysaccharides	1177:1234	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	5	4	from	Bunge	1170:1174	arg1	polysaccharides					1113:1127	polysaccharides	1113:1127	polysaccharides from the plant Anemarrhena asphodeloides Bunge	1113:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	5	4	from	Bunge	1170:1174	arg1	quantitation					1072:1083	quantitation	1072:1083	quantitation	1072:1083	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	5	4	from	Bunge	1170:1174	arg1	analysis					1101:1108	fingerprint analysis	1089:1108	fingerprint analysis	1089:1108	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	2	5	dep	sensitivity	520:530	arg1	the					506:508	the	506:508	the	506:508	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	2	6	theme	uronic	556:561	arg1	acids					563:567	uronic acids	556:567	uronic acids	556:567	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	1	7	theme	gas	210:212	arg1	spectrometry					234:245	gas chromatography mass spectrometry	210:245	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	7	theme	gas	210:212	arg1	GCMS					248:251	GCMS	248:251	GCMS	248:251	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	7	8	theme	quantitative	1486:1497	arg1	analysis					1499:1506	qualitative and quantitative analysis	1470:1506	qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1470:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	1	9	theme	simultaneous	306:317	arg1	separation					328:337	simultaneous baseline separation	306:337	simultaneous baseline separation	306:337	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	0	10	theme	Bunge	149:153	arg1	polysaccharides					155:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	3	11	theme	TMSA	772:775	arg1	derivatives					777:787	TMSA derivatives	772:787	TMSA derivatives	772:787	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	7	12	theme	traditional	1538:1548	arg1	medicines					1558:1566	traditional Chinese medicines	1538:1566	traditional Chinese medicines	1538:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	5	13	theme	plant	1138:1142	arg1	Bunge					1170:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	2	14	dep	Using	412:416	arg1	whereas					612:618	whereas	612:618	whereas	612:618	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	6	15	theme	other	1275:1279	arg1	types					1286:1290	other four types	1275:1290	other four types of derivatizations	1275:1309	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	1	16	theme	baseline	319:326	arg1	separation					328:337	simultaneous baseline separation	306:337	simultaneous baseline separation	306:337	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	0	17	theme	asphodeloides	135:147	arg1	polysaccharides					155:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Anemarrhena asphodeloides Bunge polysaccharides	123:169	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	2	18	theme	neutral	657:663	arg1	saccharides					665:675	neutral saccharides	657:675	neutral saccharides	657:675	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	5	19	from	analysis	1101:1108	arg1	Bunge					1170:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	6	20	theme	A.	1203:1204	arg1	polysaccharides					1220:1234	A. asphodeloides polysaccharides	1203:1234	A. asphodeloides polysaccharides	1203:1234	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	7	21	theme	qualitative	1470:1480	arg1	analysis					1499:1506	qualitative and quantitative analysis	1470:1506	qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1470:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	1	22	theme	chromatography	214:227	arg1	spectrometry					234:245	gas chromatography mass spectrometry	210:245	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	22	theme	chromatography	214:227	arg1	GCMS					248:251	GCMS	248:251	GCMS	248:251	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	0	23	theme	polysaccharides	155:169	arg1	quantitation					82:93	quantitation	82:93	quantitation	82:93	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	0	23	theme	polysaccharides	155:169	arg1	analysis					111:118	fingerprint analysis	99:118	fingerprint analysis	99:118	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	3	24	theme	GCMS	800:803	arg1	chromatograms					805:817	GCMS chromatograms	800:817	GCMS chromatograms	800:817	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	4	25	theme	ratios	928:933	arg1	effects					878:884	The effects	874:884	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives	874:989	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	6	26	theme	derivatizations	1295:1309	arg1	types					1286:1290	other four types	1275:1290	other four types of derivatizations	1275:1309	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	6	26	theme	derivatizations	1295:1309	arg1	TMSA					1266:1269	TMSA	1266:1269	TMSA	1266:1269	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	1	27	theme	mass	229:232	arg1	spectrometry					234:245	gas chromatography mass spectrometry	210:245	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	27	theme	mass	229:232	arg1	GCMS					248:251	GCMS	248:251	GCMS	248:251	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	2	28	theme	detection	510:518	arg1	sensitivity					520:530	detection sensitivity	510:530	detection sensitivity	510:530	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	7	29	theme	fingerprint	1402:1412	arg1	analysis					1414:1421	fingerprint analysis	1402:1421	fingerprint analysis	1402:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	5	30	theme	GCMS	1031:1034	arg1	method					1036:1041	The established GCMS method	1015:1041	The established GCMS method	1015:1041	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	4	31	theme	TMSA	974:977	arg1	derivatives					979:989	TMSA derivatives	974:989	TMSA derivatives	974:989	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	1	32	theme	spectrometry	234:245	arg1	derivatives					290:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives	210:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	0	33	theme	Gas	0:2	arg1	chromatography-mass					4:22	Gas chromatography-mass	0:22	Gas chromatography-mass	0:22	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	3	34	theme	subsequent	748:757	arg1	formation					759:767	the subsequent formation	744:767	the subsequent formation of TMSA derivatives	744:787	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	6	35	theme	comparative	1179:1189	arg1	analysis					1191:1198	A comparative analysis	1177:1198	A comparative analysis of A. asphodeloides polysaccharides	1177:1234	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	0	36	theme	trimethylsilyl-alditol	43:64	arg1	derivatives					66:76	trimethylsilyl-alditol derivatives	43:76	trimethylsilyl-alditol derivatives	43:76	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	2	37	theme	obvious	623:629	arg1	effects					631:637	no obvious effects	620:637	no obvious effects	620:637	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	7	38	theme	plant	1511:1515	arg1	polysaccharides					1517:1531	plant polysaccharides	1511:1531	plant polysaccharides from traditional Chinese medicines	1511:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	5	39	theme	asphodeloides	1156:1168	arg1	Bunge					1170:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	2	40	theme	de-lactonization	594:609	arg1	occurrence					580:589	occurrence	580:589	occurrence of de-lactonization	580:609	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	6	41	theme	asphodeloides	1206:1218	arg1	polysaccharides					1220:1234	A. asphodeloides polysaccharides	1203:1234	A. asphodeloides polysaccharides	1203:1234	A comparative analysis of A. asphodeloides polysaccharides was further performed between TMSA and other four types of derivatizations.					
30092985	7	42	from	medicines	1558:1566	arg1	polysaccharides					1517:1531	plant polysaccharides	1511:1531	plant polysaccharides from traditional Chinese medicines	1511:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	7	42	from	medicines	1558:1566	arg1	analysis					1499:1506	qualitative and quantitative analysis	1470:1506	qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1470:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	2	43	theme	alkaline	423:430	arg1	conditions					432:441	mild alkaline conditions	418:441	mild alkaline conditions	418:441	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	7	44	theme	GCMS	1336:1339	arg1	technique					1456:1464	a comprehensive and effective technique	1426:1464	a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1426:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	7	44	theme	GCMS	1336:1339	arg1	analysis					1341:1348	GCMS analysis	1336:1348	GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis	1336:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	3	45	theme	single	834:839	arg1	peak					841:844	a single peak	832:844	a single peak for each derivatized sugar	832:871	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	46	theme	aldoses	732:738	arg1	aldoses					732:738	aldoses	732:738	aldoses	732:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	46	theme	aldoses	732:738	arg1	group					723:727	the carbonyl group	710:727	the carbonyl group of aldoses	710:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	4	47	theme	temperatures	903:914	arg1	effects					878:884	The effects	874:884	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives	874:989	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	1	48	theme	-based	253:258	arg1	derivatives					290:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives	210:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	2	49	theme	sample	536:541	arg1	stability					543:551	sample stability	536:551	sample stability	536:551	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	1	50	theme	neutral	358:364	arg1	saccharides					366:376	8 neutral saccharides	356:376	8 neutral saccharides	356:376	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	3	51	theme	carbonyl	714:721	arg1	aldoses					732:738	aldoses	732:738	aldoses	732:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	51	theme	carbonyl	714:721	arg1	group					723:727	the carbonyl group	710:727	the carbonyl group of aldoses	710:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	2	52	dep	sample	459:464	arg1	both					501:504	both	501:504	both	501:504	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	7	53	theme	Chinese	1550:1556	arg1	medicines					1558:1566	traditional Chinese medicines	1538:1566	traditional Chinese medicines	1538:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	1	54	theme	trimethylsilyl-alditol	260:281	arg1	derivatives					290:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives	210:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	55	theme	saccharides	366:376	arg1	detection					343:351	detection	343:351	detection	343:351	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	1	55	theme	saccharides	366:376	arg1	separation					328:337	simultaneous baseline separation	306:337	simultaneous baseline separation	306:337	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	4	56	theme	derivatives	979:989	arg1	formations					960:969	formations	960:969	formations of TMSA derivatives	960:989	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	2	57	theme	acids	563:567	arg1	sensitivity					520:530	detection sensitivity	510:530	detection sensitivity	510:530	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	2	57	theme	acids	563:567	arg1	stability					543:551	sample stability	536:551	sample stability	536:551	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	4	58	theme	solvent	920:926	arg1	ratios					928:933	solvent ratios	920:933	solvent ratios between HMDS and TMCS	920:955	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	3	59	theme	derivatives	777:787	arg1	reduction					697:705	Sodium borohydride reduction	678:705	Sodium borohydride reduction of the carbonyl group of aldoses	678:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	59	theme	derivatives	777:787	arg1	formation					759:767	the subsequent formation	744:767	the subsequent formation of TMSA derivatives	744:787	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	60	theme	group	723:727	arg1	reduction					697:705	Sodium borohydride reduction	678:705	Sodium borohydride reduction of the carbonyl group of aldoses	678:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	3	60	theme	group	723:727	arg1	formation					759:767	the subsequent formation	744:767	the subsequent formation of TMSA derivatives	744:787	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	5	61	theme	Anemarrhena	1144:1154	arg1	Bunge					1170:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	4	62	theme	reaction	894:901	arg1	temperatures					903:914	reaction temperatures	894:914	reaction temperatures	894:914	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	3	63	theme	derivatized	855:865	arg1	sugar					867:871	each derivatized sugar	850:871	each derivatized sugar	850:871	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	7	64	theme	polysaccharides	1517:1531	arg1	analysis					1499:1506	qualitative and quantitative analysis	1470:1506	qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1470:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	5	65	theme	fingerprint	1089:1099	arg1	analysis					1101:1108	fingerprint analysis	1089:1108	fingerprint analysis	1089:1108	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	3	66	theme	Sodium	678:683	arg1	reduction					697:705	Sodium borohydride reduction	678:705	Sodium borohydride reduction of the carbonyl group of aldoses	678:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	7	67	theme	comprehensive	1428:1440	arg1	technique					1456:1464	a comprehensive and effective technique	1426:1464	a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1426:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	7	67	theme	comprehensive	1428:1440	arg1	analysis					1341:1348	GCMS analysis	1336:1348	GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis	1336:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	5	68	theme	established	1019:1029	arg1	method					1036:1041	The established GCMS method	1015:1041	The established GCMS method	1015:1041	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	1	69	theme	TMSA	284:287	arg1	derivatives					290:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives	210:300	gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	210:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	7	70	theme	precolumn	1359:1367	arg1	derivatization					1374:1387	precolumn TMSA derivatization	1359:1387	precolumn TMSA derivatization coupled with fingerprint analysis	1359:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	7	71	theme	effective	1446:1454	arg1	technique					1456:1464	a comprehensive and effective technique	1426:1464	a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines	1426:1566	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	7	71	theme	effective	1446:1454	arg1	analysis					1341:1348	GCMS analysis	1336:1348	GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis	1336:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	3	72	theme	borohydride	685:695	arg1	reduction					697:705	Sodium borohydride reduction	678:705	Sodium borohydride reduction of the carbonyl group of aldoses	678:738	Sodium borohydride reduction of the carbonyl group of aldoses and the subsequent formation of TMSA derivatives simplifies GCMS chromatograms by producing a single peak for each derivatized sugar.					
30092985	2	73	from	sample	459:464	arg1	advance					469:475	advance	469:475	advance	469:475	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	1	74	theme	novel	189:193	arg1	approach					195:202	a novel approach	187:202	a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min	187:409	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	7	75	theme	TMSA	1369:1372	arg1	derivatization					1374:1387	precolumn TMSA derivatization	1359:1387	precolumn TMSA derivatization coupled with fingerprint analysis	1359:1421	The results showed that GCMS analysis based on precolumn TMSA derivatization coupled with fingerprint analysis is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from traditional Chinese medicines.					
30092985	0	76	theme	fingerprint	99:109	arg1	analysis					111:118	fingerprint analysis	99:118	fingerprint analysis	99:118	Gas chromatography-mass spectrometry-based trimethylsilyl-alditol derivatives for quantitation and fingerprint analysis of Anemarrhena asphodeloides Bunge polysaccharides.					
30092985	1	77	theme	uronic	384:389	arg1	acids					391:395	2 uronic acids	382:395	2 uronic acids	382:395	Here we report a novel approach using gas chromatography mass spectrometry (GCMS)-based trimethylsilyl-alditol (TMSA) derivatives for simultaneous baseline separation and detection of 8 neutral saccharides and 2 uronic acids within 25 min.					
30092985	5	78	theme	polysaccharides	1113:1127	arg1	quantitation					1072:1083	quantitation	1072:1083	quantitation	1072:1083	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	5	78	theme	polysaccharides	1113:1127	arg1	analysis					1101:1108	fingerprint analysis	1089:1108	fingerprint analysis	1089:1108	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30092985	4	79	from	effects	878:884	arg1	formations					960:969	formations	960:969	formations of TMSA derivatives	960:989	The effects of both reaction temperatures and solvent ratios between HMDS and TMCS on formations of TMSA derivatives were also investigated.					
30092985	2	80	theme	mild	418:421	arg1	conditions					432:441	mild alkaline conditions	418:441	mild alkaline conditions	418:441	Using mild alkaline conditions to dissolve the sample in advance significantly increased both the detection sensitivity and sample stability of uronic acids because of occurrence of de-lactonization, whereas no obvious effects were observed for neutral saccharides.					
30092985	5	81	from	quantitation	1072:1083	arg1	Bunge					1170:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	the plant Anemarrhena asphodeloides Bunge	1134:1174	The established GCMS method was successfully applied for quantitation and fingerprint analysis of polysaccharides from the plant Anemarrhena asphodeloides Bunge.					
30055556	2	0	theme	small	542:546	arg1	EPS					559:561	EPS	559:561	EPS	559:561	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	0	theme	small	542:546	arg1	biomass					576:582	mycelial biomass	567:582	mycelial biomass	567:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	0	theme	small	542:546	arg1	amounts					548:554	small amounts	542:554	small amounts of EPS and mycelial biomass	542:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	6	1	theme	0.3-49.2	1326:1333	arg1	%					1334:1334	%	1334:1334	%	1334:1334	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	5	2	theme	estimated	1112:1120	arg1	weight					1132:1137	an estimated molecular weight	1109:1137	an estimated molecular weight < 10 kDa	1109:1146	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	3	3	theme	charged	657:663	arg1	EPSs					665:668	different net charged EPSs	643:668	different net charged EPSs	643:668	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	3	4	theme	different	643:651	arg1	EPSs					665:668	different net charged EPSs	643:668	different net charged EPSs	643:668	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	6	5	theme	submerged	1415:1423	arg1	medium					1425:1430	a glucose-peptone submerged medium	1397:1430	a glucose-peptone submerged medium	1397:1430	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	4	6	theme	gel	786:788	arg1	chromatography					801:814	gel filtration chromatography	786:814	gel filtration chromatography	786:814	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	1	7	theme	culinary-medicinal	361:378	arg1	mushroom					380:387	the culinary-medicinal mushroom	357:387	the culinary-medicinal mushroom	357:387	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	2	8	theme	32.80	501:505	arg1	mg					507:508	mg	507:508	mg	507:508	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	7	9	theme	species	1636:1642	arg1	bodies					1608:1613	the fruiting bodies	1595:1613	the fruiting bodies of the same mushroom species grown in conventional culture	1595:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	5	10	theme	EPSs	1000:1003	arg1	composition					976:986	the carbohydrate composition	959:986	the carbohydrate composition of purified EPSs	959:1003	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	1	11	theme	peptone	191:197	arg1	effect					181:186	the effect	177:186	the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus	177:413	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	4	12	theme	EPSs	860:863	arg1	range					851:855	a range	849:855	a range of EPSs with different molecular weights	849:896	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	0	13	theme	Nitrogen	130:137	arg1	Source					139:144	the Nitrogen Source	126:144	the Nitrogen Source in the Medium	126:158	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	2	14	theme	mycelial	520:527	arg1	biomass					529:535	32.80 mg EPS/g dry mycelial biomass	501:535	32.80 mg EPS/g dry mycelial biomass	501:535	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	5	15	theme	carbohydrate	963:974	arg1	composition					976:986	the carbohydrate composition	959:986	the carbohydrate composition of purified EPSs	959:1003	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	2	16	theme	EPS/g	510:514	arg1	biomass					529:535	32.80 mg EPS/g dry mycelial biomass	501:535	32.80 mg EPS/g dry mycelial biomass	501:535	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	5	17	dep	weight	1132:1137	arg1	kDa					1144:1146	< 10 kDa	1139:1146	an estimated molecular weight < 10 kDa	1109:1146	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	18	theme	molecular	1122:1130	arg1	weight					1132:1137	an estimated molecular weight	1109:1137	an estimated molecular weight < 10 kDa	1109:1146	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	3	19	theme	Flow	730:733	arg1	column					735:740	a DEAE Sepharose Fast Flow column	708:740	a DEAE Sepharose Fast Flow column	708:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	3	20	theme	Sepharose	715:723	arg1	column					735:740	a DEAE Sepharose Fast Flow column	708:740	a DEAE Sepharose Fast Flow column	708:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	6	21	theme	diverse	1256:1262	arg1	glucose					1317:1323	6.3-80.3% glucose	1307:1323	6.3-80.3% glucose	1307:1323	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	21	theme	diverse	1256:1262	arg1	N-acetylglucosamine					1373:1391	7.6-26.3% N-acetylglucosamine	1363:1391	7.6-26.3% N-acetylglucosamine	1363:1391	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	21	theme	diverse	1256:1262	arg1	N-acetylgalactosamine					1336:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	21	theme	diverse	1256:1262	arg1	mannose					1298:1304	11.1-42.3% mannose	1287:1304	11.1-42.3% mannose	1287:1304	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	21	theme	diverse	1256:1262	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	3	22	from	fractionations	690:703	arg1	column					735:740	a DEAE Sepharose Fast Flow column	708:740	a DEAE Sepharose Fast Flow column	708:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	1	23	theme	carbohydrate	290:301	arg1	compositions					303:314	the molecular weight and carbohydrate compositions	265:314	compositions	303:314	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	7	24	theme	nitrogen	1453:1460	arg1	sources					1462:1468	various nitrogen sources	1445:1468	various nitrogen sources	1445:1468	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	2	25	theme	specific	476:483	arg1	EPSs					485:488	more specific EPSs	471:488	more specific EPSs	471:488	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	0	26	from	Characteristics	0:14	arg1	Mushroom					62:69	the Citrine Oyster Mushroom	43:69	the Citrine Oyster Mushroom	43:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	5	27	theme	main	1027:1030	arg1	%					1174:1174	95.9%	1170:1174	95.9%	1170:1174	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	27	theme	main	1027:1030	arg1	component					1032:1040	the main component	1023:1040	the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa,	1023:1147	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	27	theme	main	1027:1030	arg1	rich					1153:1156	rich	1153:1156	rich	1153:1156	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	4	28	with	EPSs	860:863	arg1	weights					890:896	different molecular weights	870:896	different molecular weights	870:896	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	2	29	theme	mycelial	567:574	arg1	biomass					576:582	mycelial biomass	567:582	mycelial biomass	567:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	7	30	theme	molecules	1559:1567	arg1	possibility					1534:1544	the possibility	1530:1544	the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture	1530:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	2	31	theme	NH4	422:424	arg1	2SO4					426:429	(NH4)2SO4	421:429	(NH4)2SO4	421:429	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	6	32	theme	%	1315:1315	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	32	theme	%	1315:1315	arg1	glucose					1317:1323	6.3-80.3% glucose	1307:1323	6.3-80.3% glucose	1307:1323	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	5	33	theme	2SO4	1091:1094	arg1	medium					1096:1101	a glucose-(NH4)2SO4 medium	1076:1101	a glucose-(NH4)2SO4 medium	1076:1101	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	0	34	theme	Exopolysaccharides	19:36	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom	0:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	0	34	theme	Exopolysaccharides	19:36	arg1	Pleurotus					72:80	Pleurotus	72:80	Pleurotus	72:80	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	1	35	theme	submerged	244:252	arg1	medium					254:259	a submerged medium	242:259	a submerged medium	242:259	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	4	36	theme	Purified	743:750	arg1	EPSs					752:755	Purified EPSs	743:755	Purified EPSs	743:755	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	6	37	theme	%	1296:1296	arg1	mannose					1298:1304	11.1-42.3% mannose	1287:1304	11.1-42.3% mannose	1287:1304	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	37	theme	%	1296:1296	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	5	38	theme	glucose-	1078:1085	arg1	medium					1096:1101	a glucose-(NH4)2SO4 medium	1076:1101	a glucose-(NH4)2SO4 medium	1076:1101	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	4	39	theme	strategy	937:944	arg1	use					925:927	the use	921:927	the use of this strategy	921:944	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	0	40	theme	Oyster	55:60	arg1	Mushroom					62:69	the Citrine Oyster Mushroom	43:69	the Citrine Oyster Mushroom	43:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	6	41	theme	7.6-26.3	1363:1370	arg1	%					1371:1371	%	1371:1371	%	1371:1371	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	1	42	theme	NH4	204:206	arg1	effect					181:186	the effect	177:186	the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus	177:413	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	6	43	theme	%	1334:1334	arg1	N-acetylgalactosamine					1336:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	43	theme	%	1334:1334	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	4	44	theme	filtration	790:799	arg1	chromatography					801:814	gel filtration chromatography	786:814	gel filtration chromatography	786:814	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	2	45	used	used	435:438	arg2	2SO4					426:429	(NH4)2SO4	421:429	(NH4)2SO4	421:429	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	1	46	theme	nitrogen	222:229	arg1	sources					231:237	nitrogen sources	222:237	nitrogen sources	222:237	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	7	47	located	found	1586:1590	arg2	those					1580:1584	those	1580:1584	those	1580:1584	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	7	47	located	found	1586:1590	arg1	bodies					1608:1613	the fruiting bodies	1595:1613	the fruiting bodies of the same mushroom species grown in conventional culture	1595:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	7	48	theme	conventional	1653:1664	arg1	culture					1666:1672	conventional culture	1653:1672	conventional culture	1653:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	5	49	from	glucose	1161:1167	arg1	%					1174:1174	95.9%	1170:1174	95.9%	1170:1174	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	49	from	glucose	1161:1167	arg1	rich					1153:1156	rich	1153:1156	rich	1153:1156	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	49	from	glucose	1161:1167	arg1	component					1032:1040	the main component	1023:1040	the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa,	1023:1147	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	3	50	theme	EPSs	665:668	arg1	production					629:638	the production	625:638	the production of different net charged EPSs	625:668	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	2	51	theme	EPS	559:561	arg1	EPS					559:561	EPS	559:561	EPS	559:561	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	51	theme	EPS	559:561	arg1	biomass					576:582	mycelial biomass	567:582	mycelial biomass	567:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	51	theme	EPS	559:561	arg1	amounts					548:554	small amounts	542:554	small amounts of EPS and mycelial biomass	542:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	5	52	from	rich	1153:1156	arg1	glucose					1161:1167	glucose	1161:1167	glucose	1161:1167	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	3	53	theme	net	653:655	arg1	EPSs					665:668	different net charged EPSs	643:668	different net charged EPSs	643:668	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	6	54	theme	glucose-peptone	1399:1413	arg1	medium					1425:1430	a glucose-peptone submerged medium	1397:1430	a glucose-peptone submerged medium	1397:1430	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	7	55	theme	mushroom	1627:1634	arg1	species					1636:1642	the same mushroom species	1618:1642	the same mushroom species grown in conventional culture	1618:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	3	56	theme	crude	684:688	arg1	fractionations					690:703	crude fractionations	684:703	crude fractionations on a DEAE Sepharose Fast Flow column	684:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	5	57	with	medium	1096:1101	arg1	weight					1132:1137	an estimated molecular weight	1109:1137	an estimated molecular weight < 10 kDa	1109:1146	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	7	58	used	used	1514:1517	arg2	results					1497:1503	these results	1491:1503	these results	1491:1503	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	4	59	theme	different	870:878	arg1	weights					890:896	different molecular weights	870:896	different molecular weights	870:896	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	2	60	theme	dry	516:518	arg1	biomass					529:535	32.80 mg EPS/g dry mycelial biomass	501:535	32.80 mg EPS/g dry mycelial biomass	501:535	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	5	61	dep	rich	1153:1156	arg1	%					1174:1174	95.9%	1170:1174	95.9%	1170:1174	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	61	dep	rich	1153:1156	arg1	component					1032:1040	the main component	1023:1040	the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa,	1023:1147	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	61	dep	rich	1153:1156	arg1	rich					1153:1156	rich	1153:1156	rich	1153:1156	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	5	62	theme	composition	976:986	arg1	Analysis					947:954	Analysis	947:954	Analysis of the carbohydrate composition of purified EPSs	947:1003	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	7	63	theme	same	1622:1625	arg1	species					1636:1642	the same mushroom species	1618:1642	the same mushroom species grown in conventional culture	1618:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	7	64	theme	submerged	1473:1481	arg1	medium					1483:1488	submerged medium	1473:1488	submerged medium	1473:1488	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	2	65	theme	mg	507:508	arg1	biomass					529:535	32.80 mg EPS/g dry mycelial biomass	501:535	32.80 mg EPS/g dry mycelial biomass	501:535	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	5	66	theme	purified	991:998	arg1	EPSs					1000:1003	purified EPSs	991:1003	purified EPSs	991:1003	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	1	67	from	effect	181:186	arg1	weight					279:284	the molecular weight and carbohydrate compositions	265:314	weight	279:284	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	1	67	from	effect	181:186	arg1	compositions					303:314	the molecular weight and carbohydrate compositions	265:314	compositions	303:314	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	3	68	theme	Fast	725:728	arg1	column					735:740	a DEAE Sepharose Fast Flow column	708:740	a DEAE Sepharose Fast Flow column	708:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	7	69	theme	fruiting	1599:1606	arg1	bodies					1608:1613	the fruiting bodies	1595:1613	the fruiting bodies of the same mushroom species grown in conventional culture	1595:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	7	70	theme	various	1445:1451	arg1	sources					1462:1468	various nitrogen sources	1445:1468	various nitrogen sources	1445:1468	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	0	71	from	Mushroom	62:69	arg1	Exopolysaccharides					19:36	Exopolysaccharides	19:36	Exopolysaccharides from the Citrine Oyster Mushroom	19:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	0	71	from	Mushroom	62:69	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom	0:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	0	71	from	Mushroom	62:69	arg1	Pleurotus					72:80	Pleurotus	72:80	Pleurotus	72:80	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	2	72	theme	biomass	576:582	arg1	EPS					559:561	EPS	559:561	EPS	559:561	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	72	theme	biomass	576:582	arg1	biomass					576:582	mycelial biomass	567:582	mycelial biomass	567:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	2	72	theme	biomass	576:582	arg1	amounts					548:554	small amounts	542:554	small amounts of EPS and mycelial biomass	542:582	When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass.					
30055556	1	73	theme	exopolysaccharides	319:336	arg1	weight					279:284	the molecular weight and carbohydrate compositions	265:314	weight	279:284	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	1	73	theme	exopolysaccharides	319:336	arg1	compositions					303:314	the molecular weight and carbohydrate compositions	265:314	compositions	303:314	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	4	74	theme	molecular	880:888	arg1	weights					890:896	different molecular weights	870:896	different molecular weights	870:896	Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy.					
30055556	5	75	dep	glucose-	1078:1085	arg1	NH4					1087:1089	NH4	1087:1089	NH4	1087:1089	Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan.					
30055556	6	76	dep	heteropolysaccharides	1264:1284	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	76	dep	heteropolysaccharides	1264:1284	arg1	N-acetylglucosamine					1373:1391	7.6-26.3% N-acetylglucosamine	1363:1391	7.6-26.3% N-acetylglucosamine	1363:1391	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	76	dep	heteropolysaccharides	1264:1284	arg1	N-acetylgalactosamine					1336:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	0.3-49.2% N-acetylgalactosamine	1326:1356	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	76	dep	heteropolysaccharides	1264:1284	arg1	mannose					1298:1304	11.1-42.3% mannose	1287:1304	11.1-42.3% mannose	1287:1304	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	76	dep	heteropolysaccharides	1264:1284	arg1	glucose					1317:1323	6.3-80.3% glucose	1307:1323	6.3-80.3% glucose	1307:1323	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	7	77	theme	similar	1569:1575	arg1	molecules					1559:1567	obtaining molecules	1549:1567	obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture	1549:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	6	78	theme	6.3-80.3	1307:1314	arg1	%					1315:1315	%	1315:1315	%	1315:1315	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	0	79	dep	Pleurotus	72:80	arg1	citrinopileatus					82:96	Pleurotus citrinopileatus	72:96	Pleurotus citrinopileatus (Agaricomycetes)	72:113	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	7	80	theme	obtaining	1549:1557	arg1	molecules					1559:1567	obtaining molecules	1549:1567	obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture	1549:1672	By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.					
30055556	0	81	theme	Citrine	47:53	arg1	Mushroom					62:69	the Citrine Oyster Mushroom	43:69	the Citrine Oyster Mushroom	43:69	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	6	82	theme	11.1-42.3	1287:1295	arg1	%					1296:1296	%	1296:1296	%	1296:1296	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	83	theme	%	1371:1371	arg1	N-acetylglucosamine					1373:1391	7.6-26.3% N-acetylglucosamine	1363:1391	7.6-26.3% N-acetylglucosamine	1363:1391	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	6	83	theme	%	1371:1371	arg1	heteropolysaccharides					1264:1284	diverse heteropolysaccharides	1256:1284	diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine)	1256:1392	Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium.					
30055556	1	84	theme	molecular	269:277	arg1	weight					279:284	the molecular weight and carbohydrate compositions	265:314	weight	279:284	We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus.					
30055556	0	85	from	Source	139:144	arg1	Medium					153:158	the Medium	149:158	the Medium	149:158	Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium.					
30055556	3	86	theme	nitrogen	591:598	arg1	sources					600:606	These nitrogen sources	585:606	These nitrogen sources	585:606	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30055556	3	87	theme	DEAE	710:713	arg1	column					735:740	a DEAE Sepharose Fast Flow column	708:740	a DEAE Sepharose Fast Flow column	708:740	These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column.					
30818370	0	0	theme	dense	80:84	arg1	glycosylation					100:112	dense β-galactoside glycosylation	80:112	dense β-galactoside glycosylation	80:112	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	2	1	theme	Plasmacytoid	329:340	arg1	pDCs					359:362	pDCs	359:362	pDCs	359:362	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	2	1	theme	Plasmacytoid	329:340	arg1	cells					352:356	Plasmacytoid dendritic cells	329:356	Plasmacytoid dendritic cells (pDCs)	329:363	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	1	2	theme	infected	311:318	arg1	T-cells					320:326	infected T-cells	311:326	infected T-cells	311:326	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	6	3	theme	cell	1137:1140	arg1	structures					1174:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	5	4	theme	surface	953:959	arg1	pattern					961:967	the cell surface pattern	944:967	the cell surface pattern of terminal β-galactoside glycosylation	944:1007	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	3	5	theme	cell	517:520	arg1	contact					522:528	physical cell contact	508:528	physical cell contact with HTLV-infected cells	508:553	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	0	6	theme	β-galactoside	86:98	arg1	glycosylation					100:112	dense β-galactoside glycosylation	80:112	dense β-galactoside glycosylation	80:112	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	4	7	theme	antiviral	857:865	arg1	response					867:874	the antiviral response	853:874	the antiviral response by pDCs	853:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	4	8	theme	glycan-rich	725:735	arg1	structure					737:745	glycan-rich structure	725:745	glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs	725:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	1	9	theme	innate	240:245	arg1	immunity					247:254	the innate immunity	236:254	the innate immunity	236:254	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	6	10	theme	surface-associated	1142:1159	arg1	structures					1174:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	4	11	theme	described	816:824	arg1	structure					832:840	a newly described viral structure	808:840	a newly described viral structure triggering the antiviral response by pDCs	808:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	6	12	gly	glycosylated	1161:1172	arg1	structures					1174:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	3	13	theme	type	484:487	arg1	interferons					491:501	type I interferons	484:501	type I interferons	484:501	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	2	14	theme	type	414:417	arg1	interferon					421:430	their robust type I interferon	401:430	their robust type I interferon production	401:441	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	1	15	from	inhibition	267:276	arg1	T-cells					320:326	infected T-cells	311:326	infected T-cells	311:326	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	3	16	theme	I	489:489	arg1	interferons					491:501	type I interferons	484:501	type I interferons	484:501	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	2	17	theme	robust	407:412	arg1	interferon					421:430	their robust type I interferon	401:430	their robust type I interferon production	401:441	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	4	18	with	present	712:718	arg1	structure					737:745	glycan-rich structure	725:745	glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs	725:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	1	19	theme	immunity	247:254	arg1	escape					226:231	viral escape	220:231	viral escape of the innate immunity	220:254	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	4	20	theme	viral	826:830	arg1	structure					832:840	a newly described viral structure	808:840	a newly described viral structure triggering the antiviral response by pDCs	808:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	4	21	theme	surface	688:694	arg1	associated-HTLV					696:710	surface associated-HTLV	688:710	surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs	688:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	0	22	theme	HTLV	27:30	arg1	Sensing					0:6	Sensing	0:6	Sensing of cell-associated HTLV by plasmacytoid dendritic cells	0:62	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	6	23	theme	glycosylated	1161:1172	arg1	structures					1174:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	3	24	theme	physical	508:515	arg1	contact					522:528	physical cell contact	508:528	physical cell contact with HTLV-infected cells	508:553	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	2	25	theme	viral	385:389	arg1	escape					391:396	viral escape	385:396	viral escape	385:396	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	0	26	theme	cell-associated	11:25	arg1	HTLV					27:30	cell-associated HTLV	11:30	cell-associated HTLV	11:30	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	6	27	theme	immunity	1213:1220	arg1	activation					1192:1201	the activation	1188:1201	the activation of innate immunity	1188:1220	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	1	28	theme	Human	115:119	arg1	infection					149:157	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	0	29	theme	plasmacytoid	35:46	arg1	cells					58:62	plasmacytoid dendritic cells	35:62	plasmacytoid dendritic cells	35:62	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	5	30	theme	heparan	899:905	arg1	proteoglycans					915:927	heparan sulfate proteoglycans	899:927	heparan sulfate proteoglycans	899:927	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	5	31	theme	immunostimulatory	1043:1059	arg1	RNA					1061:1063	the immunostimulatory RNA	1039:1063	the immunostimulatory RNA	1039:1063	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	2	32	theme	interferon	421:430	arg1	production					432:441	their robust type I interferon production	401:441	their robust type I interferon production	401:441	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	3	33	with	contact	522:528	arg1	cells					549:553	HTLV-infected cells	535:553	HTLV-infected cells	535:553	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	1	34	theme	type	281:284	arg1	interferon					288:297	type I interferon	281:297	type I interferon response	281:306	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	3	35	theme	HTLV-infected	535:547	arg1	cells					549:553	HTLV-infected cells	535:553	HTLV-infected cells	535:553	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	6	36	theme	virus-containing	1120:1135	arg1	structures					1174:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	virus-containing cell surface-associated glycosylated structures	1120:1183	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	1	37	theme	T	121:121	arg1	virus					136:140	T Lymphotropic virus	121:140	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	1	37	theme	T	121:121	arg1	HTLV					143:146	HTLV	143:146	HTLV	143:146	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	0	38	theme	dendritic	48:56	arg1	cells					58:62	plasmacytoid dendritic cells	35:62	plasmacytoid dendritic cells	35:62	Sensing of cell-associated HTLV by plasmacytoid dendritic cells is regulated by dense β-galactoside glycosylation.					
30818370	6	39	theme	innate	1206:1211	arg1	immunity					1213:1220	innate immunity	1206:1220	innate immunity	1206:1220	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	3	40	theme	cells	635:639	arg1	ability					605:611	the ability	601:611	the ability of the HTLV-producing cells to transmit infection	601:661	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	5	41	theme	RNA	1061:1063	arg1	transmission					1023:1034	the transmission	1019:1034	the transmission of the immunostimulatory RNA to pDCs	1019:1071	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	1	42	theme	Lymphotropic	123:134	arg1	virus					136:140	T Lymphotropic virus	121:140	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	1	42	theme	Lymphotropic	123:134	arg1	HTLV					143:146	HTLV	143:146	HTLV	143:146	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	1	43	theme	interferon	288:297	arg1	response					299:306	type I interferon response	281:306	type I interferon response	281:306	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	5	44	theme	β-galactoside	981:993	arg1	glycosylation					995:1007	terminal β-galactoside glycosylation	972:1007	terminal β-galactoside glycosylation	972:1007	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	4	45	theme	biofilm-like	762:773	arg1	structure					775:783	biofilm-like structure	762:783	biofilm-like structure	762:783	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	1	46	theme	virus	136:140	arg1	infection					149:157	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection	115:157	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	6	47	theme	structures	1174:1183	arg1	function					1108:1115	a function	1106:1115	a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity	1106:1220	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	5	48	theme	terminal	972:979	arg1	glycosylation					995:1007	terminal β-galactoside glycosylation	972:1007	terminal β-galactoside glycosylation	972:1007	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	5	49	theme	glycosylation	995:1007	arg1	pattern					961:967	the cell surface pattern	944:967	the cell surface pattern of terminal β-galactoside glycosylation	944:1007	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	5	49	theme	glycosylation	995:1007	arg1	proteoglycans					915:927	heparan sulfate proteoglycans	899:927	heparan sulfate proteoglycans	899:927	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	4	50	theme	present	712:718	arg1	associated-HTLV					696:710	surface associated-HTLV	688:710	surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs	688:882	We show that pDCs sense surface associated-HTLV present with glycan-rich structure referred to as biofilm-like structure, which thus represents a newly described viral structure triggering the antiviral response by pDCs.					
30818370	5	51	theme	sulfate	907:913	arg1	proteoglycans					915:927	heparan sulfate proteoglycans	899:927	heparan sulfate proteoglycans	899:927	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	3	52	theme	HTLV-producing	620:633	arg1	cells					635:639	the HTLV-producing cells	616:639	the HTLV-producing cells	616:639	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	3	53	theme	pDC	560:562	arg1	activation					564:573	pDC activation	560:573	pDC activation	560:573	Here, we demonstrated that pDCs produce type I interferons upon physical cell contact with HTLV-infected cells, yet pDC activation inversely correlates with the ability of the HTLV-producing cells to transmit infection.					
30818370	5	54	theme	cell	948:951	arg1	pattern					961:967	the cell surface pattern	944:967	the cell surface pattern of terminal β-galactoside glycosylation	944:1007	Consistently, heparan sulfate proteoglycans and especially the cell surface pattern of terminal β-galactoside glycosylation, modulate the transmission of the immunostimulatory RNA to pDCs.					
30818370	1	55	theme	response	299:306	arg1	inhibition					267:276	inhibition	267:276	inhibition of type I interferon response in infected T-cells	267:326	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30818370	6	56	from	function	1108:1115	arg1	activation					1192:1201	the activation	1188:1201	the activation of innate immunity	1188:1220	Altogether, our results uncover a function of virus-containing cell surface-associated glycosylated structures in the activation of innate immunity.					
30818370	2	57	theme	dendritic	342:350	arg1	pDCs					359:362	pDCs	359:362	pDCs	359:362	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	2	57	theme	dendritic	342:350	arg1	cells					352:356	Plasmacytoid dendritic cells	329:356	Plasmacytoid dendritic cells (pDCs)	329:363	Plasmacytoid dendritic cells (pDCs) are known to bypass viral escape by their robust type I interferon production.					
30818370	1	58	theme	viral	220:224	arg1	escape					226:231	viral escape	220:231	viral escape of the innate immunity	220:254	Human T Lymphotropic virus (HTLV) infection can persist in individuals resulting, at least in part, from viral escape of the innate immunity, including inhibition of type I interferon response in infected T-cells.					
30805695	8	0	theme	health	2109:2114	arg1	benefits					2116:2123	health benefits	2109:2123	health benefits	2109:2123	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	4	1	dep	showed	1018:1023	arg1	48 h					1193:1196	48 h	1193:1196	48 h	1193:1196	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	5	2	theme	bacterial	1421:1429	arg1	populations					1431:1441	human fecal bacterial populations	1409:1441	human fecal bacterial populations	1409:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	6	3	theme	acid	1541:1544	arg1	higher					1580:1585	higher	1580:1585	higher	1580:1585	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	6	3	theme	acid	1541:1544	arg1	concentrations					1486:1499	The concentrations	1482:1499	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA	1482:1559	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	1	4	theme	fiber	249:253	arg1	source					239:244	a rich source	232:244	a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	232:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	4	theme	fiber	249:253	arg1	seaweeds					207:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	3	5	theme	depolymerized	718:730	arg1	DE					744:745	DE	744:745	DE	744:745	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	5	theme	depolymerized	718:730	arg1	extract					735:741	a depolymerized CE extract	716:741	a depolymerized CE extract (DE)	716:746	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	4	6	theme	significant	1028:1038	arg1	changes					1040:1046	no significant changes	1025:1046	no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE	1025:1110	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	0	7	attach	derived	41:47	arg2	extract					33:39	a polysaccharide-rich extract	11:39	a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata	11:85	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	0	7	attach	derived	41:47	arg1	digitata					78:85	Irish-sourced Laminaria digitata	54:85	Irish-sourced Laminaria digitata	54:85	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	4	8	theme	time	1154:1157	arg1	point					1159:1163	any time point	1150:1163	any time point measured	1150:1172	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	5	9	theme	human	1409:1413	arg1	populations					1431:1441	human fecal bacterial populations	1409:1441	human fecal bacterial populations	1409:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	6	10	theme	propionic	1517:1525	arg1	acid					1527:1530	propionic acid	1517:1530	propionic acid	1517:1530	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	7	11	theme	propionate	1708:1717	arg1	ratio					1719:1723	the acetate:propionate ratio	1696:1723	the acetate:propionate ratio	1696:1723	Furthermore, the acetate:propionate ratio was significantly reduced (p < 0.05) for both CD and DE following 24, 36 and 48 h fermentation.					
30805695	8	12	theme	microbiota-associated	1836:1856	arg1	changes					1886:1892	The microbiota-associated metabolic and compositional changes	1832:1892	The microbiota-associated metabolic and compositional changes noted	1832:1898	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	6	13	theme	acid	1511:1514	arg1	higher					1580:1585	higher	1580:1585	higher	1580:1585	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	6	13	theme	acid	1511:1514	arg1	concentrations					1486:1499	The concentrations	1482:1499	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA	1482:1559	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	1	14	theme	Brown	201:205	arg1	source					239:244	a rich source	232:244	a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	232:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	14	theme	Brown	201:205	arg1	seaweeds					207:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	2	15	from	effect	493:498	arg1	microbiota					555:564	the microbiota	551:564	the microbiota	551:564	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	4	16	theme	cellulose-negative	1120:1137	arg1	control					1139:1145	the cellulose-negative control	1116:1145	the cellulose-negative control	1116:1145	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	0	17	theme	human	136:140	arg1	microbiota					146:155	the human gut microbiota	132:155	the human gut microbiota	132:155	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	4	18	theme	cultured	1051:1058	arg1	lactobacilli					1060:1071	lactobacilli	1060:1071	lactobacilli	1060:1071	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	8	19	theme	initial	1908:1914	arg1	indication					1916:1925	initial indication	1908:1925	initial indication of putative beneficial health benefits of L. digitata	1908:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	3	20	theme	gut	755:757	arg1	composition					770:780	the gut microbiota composition	751:780	the gut microbiota composition	751:780	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	0	21	theme	microbiota	146:155	arg1	composition					94:104	composition	94:104	composition	94:104	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	0	21	theme	microbiota	146:155	arg1	activity					120:127	metabolic activity	110:127	metabolic activity	110:127	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	3	22	theme	metagenomic	848:858	arg1	analysis					874:881	metagenomic compositional analysis	848:881	metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing	848:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	2	23	theme	collective	507:516	arg1	component					538:546	the collective brown seaweed fiber component	503:546	the collective brown seaweed fiber component	503:546	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	7	24	theme	acetate	1700:1706	arg1	ratio					1719:1723	the acetate:propionate ratio	1696:1723	the acetate:propionate ratio	1696:1723	Furthermore, the acetate:propionate ratio was significantly reduced (p < 0.05) for both CD and DE following 24, 36 and 48 h fermentation.					
30805695	0	25	theme	Laminaria	68:76	arg1	digitata					78:85	Irish-sourced Laminaria digitata	54:85	Irish-sourced Laminaria digitata	54:85	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	6	26	theme	total	1550:1554	arg1	SCFA					1556:1559	total SCFA	1550:1559	total SCFA	1550:1559	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	3	27	theme	16S	889:891	arg1	pyrosequencing					911:924	16S rRNA FLX amplicon pyrosequencing	889:924	16S rRNA FLX amplicon pyrosequencing	889:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	4	28	theme	Selective	991:999	arg1	analysis					1009:1016	Selective culture analysis	991:1016	Selective culture analysis	991:1016	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	3	29	theme	batch	821:825	arg1	model					835:839	an in vitro fecal batch culture model	803:839	an in vitro fecal batch culture model	803:839	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	8	30	theme	putative	1930:1937	arg1	benefits					1957:1964	putative beneficial health benefits	1930:1964	putative beneficial health benefits of L. digitata	1930:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	2	31	theme	component	538:546	arg1	effect					493:498	the effect	489:498	the effect of the collective brown seaweed fiber component on the microbiota	489:564	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	0	32	from	Effects	0:6	arg1	composition					94:104	composition	94:104	composition	94:104	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	0	32	from	Effects	0:6	arg1	activity					120:127	metabolic activity	110:127	metabolic activity	110:127	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	8	33	theme	health	1950:1955	arg1	benefits					1957:1964	putative beneficial health benefits	1930:1964	putative beneficial health benefits of L. digitata	1930:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	6	34	dep	CE	1600:1601	arg1	the					1596:1598	the	1596:1598	the	1596:1598	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	2	35	theme	seaweed	524:530	arg1	component					538:546	the collective brown seaweed fiber component	503:546	the collective brown seaweed fiber component	503:546	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	3	36	from	effect	628:633	arg1	metabolism					786:795	metabolism	786:795	metabolism	786:795	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	36	from	effect	628:633	arg1	composition					770:780	the gut microbiota composition	751:780	the gut microbiota composition	751:780	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	0	37	theme	metabolic	110:118	arg1	activity					120:127	metabolic activity	110:127	metabolic activity	110:127	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	0	38	dep	composition	94:104	arg1	the					90:92	the	90:92	the	90:92	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	3	39	theme	FLX	898:900	arg1	pyrosequencing					911:924	16S rRNA FLX amplicon pyrosequencing	889:924	16S rRNA FLX amplicon pyrosequencing	889:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	8	40	theme	digitata	1972:1979	arg1	benefits					1957:1964	putative beneficial health benefits	1930:1964	putative beneficial health benefits of L. digitata	1930:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	3	41	theme	acid	948:951	arg1	analysis					960:967	short-chain fatty acid (SCFA) analysis	930:967	short-chain fatty acid (SCFA) analysis using GC-FID	930:980	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	2	42	theme	gut	442:444	arg1	composition					457:467	the gut microbiota composition	438:467	the gut microbiota composition	438:467	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	4	43	dep	RESULTS	983:989	arg1	showed					1018:1023	showed	1018:1023	showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h)	1018:1197	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	4	44	from	changes	1040:1046	arg1	bifidobacteria					1076:1089	bifidobacteria	1076:1089	bifidobacteria	1076:1089	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	4	44	from	changes	1040:1046	arg1	lactobacilli					1060:1071	lactobacilli	1060:1071	lactobacilli	1060:1071	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	4	44	from	changes	1040:1046	arg1	DE					1109:1110	DE	1109:1110	DE	1109:1110	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	3	45	theme	short-chain	930:940	arg1	SCFA					954:957	SCFA	954:957	SCFA	954:957	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	45	theme	short-chain	930:940	arg1	acid					948:951	short-chain fatty acid	930:951	short-chain fatty acid (SCFA) analysis using GC-FID	930:980	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	8	46	theme	L.	2033:2034	arg1	fiber					2053:2057	L. digitata-derived fiber	2033:2057	L. digitata-derived fiber	2033:2057	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	0	47	theme	extract	33:39	arg1	Effects					0:6	Effects	0:6	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota	0:155	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	1	48	theme	several	276:282	arg1	fucoidan					336:343	fucoidan	336:343	fucoidan	336:343	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	48	theme	several	276:282	arg1	laminarin					325:333	laminarin	325:333	laminarin	325:333	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	48	theme	several	276:282	arg1	polysaccharides					299:313	several non-digestible polysaccharides	276:313	several non-digestible polysaccharides including laminarin, fucoidan and alginate	276:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	48	theme	several	276:282	arg1	alginate					349:356	alginate	349:356	alginate	349:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	3	49	theme	polysaccharide-rich	646:664	arg1	extract					666:672	a crude polysaccharide-rich extract	638:672	a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE)	638:746	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	1	50	theme	polysaccharides	299:313	arg1	presence					264:271	the presence	260:271	the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	260:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	3	51	theme	Laminaria	688:696	arg1	CE					708:709	CE	708:709	CE	708:709	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	51	theme	Laminaria	688:696	arg1	digitata					698:705	Laminaria digitata	688:705	Laminaria digitata (CE)	688:710	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	2	52	theme	individual	365:374	arg1	polysaccharides					376:390	These individual polysaccharides	359:390	These individual polysaccharides	359:390	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	8	53	theme	compositional	1872:1884	arg1	changes					1886:1892	The microbiota-associated metabolic and compositional changes	1832:1892	The microbiota-associated metabolic and compositional changes noted	1832:1898	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	0	54	theme	colonic	175:181	arg1	model					183:187	an in vitro colonic model	163:187	an in vitro colonic model	163:187	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	4	55	from	control	1139:1145	arg1	bifidobacteria					1076:1089	bifidobacteria	1076:1089	bifidobacteria	1076:1089	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	4	55	from	control	1139:1145	arg1	lactobacilli					1060:1071	lactobacilli	1060:1071	lactobacilli	1060:1071	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	4	55	from	control	1139:1145	arg1	DE					1109:1110	DE	1109:1110	DE	1109:1110	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	1	56	theme	rich	234:237	arg1	source					239:244	a rich source	232:244	a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	232:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	56	theme	rich	234:237	arg1	seaweeds					207:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	5	57	theme	populations	1431:1441	arg1	abundance					1281:1289	the relative abundance	1268:1289	the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations	1268:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	6	58	theme	butyric	1533:1539	arg1	acid					1541:1544	butyric acid	1533:1544	butyric acid	1533:1544	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	3	59	dep	METHODS	592:598	arg1	investigated					611:622	investigated	611:622	investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID	611:980	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	60	theme	CE	732:733	arg1	DE					744:745	DE	744:745	DE	744:745	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	60	theme	CE	732:733	arg1	extract					735:741	a depolymerized CE extract	716:741	a depolymerized CE extract (DE)	716:746	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	6	61	theme	acid	1527:1530	arg1	higher					1580:1585	higher	1580:1585	higher	1580:1585	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	6	61	theme	acid	1527:1530	arg1	concentrations					1486:1499	The concentrations	1482:1499	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA	1482:1559	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	8	62	theme	metabolic	1858:1866	arg1	changes					1886:1892	The microbiota-associated metabolic and compositional changes	1832:1892	The microbiota-associated metabolic and compositional changes noted	1832:1898	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	5	63	theme	fecal	1415:1419	arg1	populations					1431:1441	human fecal bacterial populations	1409:1441	human fecal bacterial populations	1409:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	1	64	theme	BACKGROUND	190:199	arg1	source					239:244	a rich source	232:244	a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	232:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	64	theme	BACKGROUND	190:199	arg1	seaweeds					207:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds	190:214	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	0	65	theme	gut	142:144	arg1	microbiota					146:155	the human gut microbiota	132:155	the human gut microbiota	132:155	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	6	66	theme	acetic	1504:1509	arg1	acid					1511:1514	acetic acid	1504:1514	acetic acid	1504:1514	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	3	67	dep	in	806:807	arg1	vitro					809:813	vitro	809:813	vitro	809:813	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	0	68	dep	in	166:167	arg1	vitro					169:173	vitro	169:173	vitro	169:173	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	3	69	theme	microbiota	759:768	arg1	composition					770:780	the gut microbiota composition	751:780	the gut microbiota composition	751:780	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	0	70	theme	in	166:167	arg1	model					183:187	an in vitro colonic model	163:187	an in vitro colonic model	163:187	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	3	71	theme	compositional	860:872	arg1	analysis					874:881	metagenomic compositional analysis	848:881	metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing	848:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	8	72	link	digitata-derived	2036:2051	arg1	fiber					2053:2057	L. digitata-derived fiber	2033:2057	L. digitata-derived fiber	2033:2057	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	3	73	theme	in	806:807	arg1	model					835:839	an in vitro fecal batch culture model	803:839	an in vitro fecal batch culture model	803:839	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	6	74	theme	SCFA	1556:1559	arg1	higher					1580:1585	higher	1580:1585	higher	1580:1585	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	6	74	theme	SCFA	1556:1559	arg1	concentrations					1486:1499	The concentrations	1482:1499	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA	1482:1559	The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05).					
30805695	8	75	dep	CONCLUSION	1821:1830	arg1	provide					1900:1906	provide	1900:1906	provide initial indication of putative beneficial health benefits of L. digitata in vitro	1900:1988	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	5	76	theme	metagenomic	1210:1220	arg1	analysis					1222:1229	metagenomic analysis	1210:1229	metagenomic analysis	1210:1229	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	8	77	theme	beneficial	1939:1948	arg1	benefits					1957:1964	putative beneficial health benefits	1930:1964	putative beneficial health benefits of L. digitata	1930:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	3	78	theme	rRNA	893:896	arg1	pyrosequencing					911:924	16S rRNA FLX amplicon pyrosequencing	889:924	16S rRNA FLX amplicon pyrosequencing	889:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	79	theme	culture	827:833	arg1	model					835:839	an in vitro fecal batch culture model	803:839	an in vitro fecal batch culture model	803:839	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	8	80	theme	benefits	1957:1964	arg1	indication					1916:1925	initial indication	1908:1925	initial indication of putative beneficial health benefits of L. digitata	1908:1979	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	2	81	theme	fiber	532:536	arg1	component					538:546	the collective brown seaweed fiber component	503:546	the collective brown seaweed fiber component	503:546	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	3	82	theme	fecal	815:819	arg1	model					835:839	an in vitro fecal batch culture model	803:839	an in vitro fecal batch culture model	803:839	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	2	83	theme	brown	518:522	arg1	component					538:546	the collective brown seaweed fiber component	503:546	the collective brown seaweed fiber component	503:546	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	5	84	theme	families	1302:1309	arg1	abundance					1281:1289	the relative abundance	1268:1289	the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations	1268:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	3	85	theme	amplicon	902:909	arg1	pyrosequencing					911:924	16S rRNA FLX amplicon pyrosequencing	889:924	16S rRNA FLX amplicon pyrosequencing	889:924	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	2	86	theme	microbiota	446:455	arg1	composition					457:467	the gut microbiota composition	438:467	the gut microbiota composition	438:467	These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.					
30805695	0	87	theme	polysaccharide-rich	13:31	arg1	extract					33:39	a polysaccharide-rich extract	11:39	a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata	11:85	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	8	88	theme	digitata-derived	2036:2051	arg1	fiber					2053:2057	L. digitata-derived fiber	2033:2057	L. digitata-derived fiber	2033:2057	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	3	89	theme	extract	666:672	arg1	effect					628:633	the effect	624:633	the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism	624:795	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	5	90	theme	relative	1272:1279	arg1	abundance					1281:1289	the relative abundance	1268:1289	the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations	1268:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	5	91	theme	several	1294:1300	arg1	Ruminococcus					1373:1384	Ruminococcus	1373:1384	Ruminococcus	1373:1384	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	5	91	theme	several	1294:1300	arg1	Lachnospiraceae					1321:1335	Lachnospiraceae	1321:1335	Lachnospiraceae	1321:1335	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	5	91	theme	several	1294:1300	arg1	families					1302:1309	several families	1294:1309	several families including Lachnospiraceae	1294:1335	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	5	91	theme	several	1294:1300	arg1	Parabacteroides					1390:1404	Parabacteroides	1390:1404	Parabacteroides	1390:1404	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	5	91	theme	several	1294:1300	arg1	Streptococcus					1358:1370	Streptococcus	1358:1370	Streptococcus	1358:1370	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
30805695	3	92	theme	fatty	942:946	arg1	SCFA					954:957	SCFA	954:957	SCFA	954:957	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	3	92	theme	fatty	942:946	arg1	acid					948:951	short-chain fatty acid	930:951	short-chain fatty acid (SCFA) analysis using GC-FID	930:980	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	4	93	theme	culture	1001:1007	arg1	analysis					1009:1016	Selective culture analysis	991:1016	Selective culture analysis	991:1016	RESULTS Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h).					
30805695	3	94	theme	crude	640:644	arg1	extract					666:672	a crude polysaccharide-rich extract	638:672	a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE)	638:746	METHODS This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.					
30805695	0	95	theme	Irish-sourced	54:66	arg1	digitata					78:85	Irish-sourced Laminaria digitata	54:85	Irish-sourced Laminaria digitata	54:85	Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.					
30805695	1	96	theme	non-digestible	284:297	arg1	fucoidan					336:343	fucoidan	336:343	fucoidan	336:343	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	96	theme	non-digestible	284:297	arg1	laminarin					325:333	laminarin	325:333	laminarin	325:333	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	96	theme	non-digestible	284:297	arg1	polysaccharides					299:313	several non-digestible polysaccharides	276:313	several non-digestible polysaccharides including laminarin, fucoidan and alginate	276:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	96	theme	non-digestible	284:297	arg1	alginate					349:356	alginate	349:356	alginate	349:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	1	97	with	source	239:244	arg1	presence					264:271	the presence	260:271	the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate	260:356	BACKGROUND Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate.					
30805695	8	98	theme	gut	2083:2085	arg1	microbiota					2087:2096	the gut microbiota	2079:2096	the gut microbiota	2079:2096	CONCLUSION The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.					
30805695	7	99	dep	reduced	1743:1749	arg1	p < 0.05					1752:1759	p < 0.05	1752:1759	p < 0.05	1752:1759	Furthermore, the acetate:propionate ratio was significantly reduced (p < 0.05) for both CD and DE following 24, 36 and 48 h fermentation.					
30805695	5	100	theme	genera	1341:1346	arg1	abundance					1281:1289	the relative abundance	1268:1289	the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations	1268:1441	Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h.					
31311856	0	0	theme	O-glycoprotein	82:95	arg1	pathway					109:115	an O-glycoprotein degradation pathway	79:115	an O-glycoprotein degradation pathway	79:115	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	6	1	theme	critical	1325:1332	arg1	insights					1334:1341	critical insights	1325:1341	critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins	1325:1420	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	6	2	theme	O-glycoproteins	1290:1304	arg1	degradation					1275:1285	the degradation	1271:1285	the degradation of O-glycoproteins in yeast	1271:1313	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	3	3	contain	containing	729:738	arg2	source					762:767	the carbon source	751:767	the carbon source	751:767	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	3	3	contain	containing	729:738	arg2	mannose					740:746	mannose	740:746	mannose	740:746	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	3	3	contain	containing	729:738	arg1	medium					722:727	medium	722:727	medium containing mannose as the carbon source	722:767	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	6	4	from	evidence	1224:1231	arg1	support					1236:1242	support	1236:1242	support of a regulated pathway for the degradation of O-glycoproteins in yeast	1236:1313	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	5	5	theme	severe	1089:1094	arg1	defect					1103:1108	a severe growth defect	1087:1108	a severe growth defect	1087:1108	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	0	6	theme	pathway	109:115	arg1	presence					67:74	the presence	63:74	the presence of an O-glycoprotein degradation pathway in yeast	63:124	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	1	7	theme	donor	396:400	arg1	oligosaccharides					378:393	lipid-linked oligosaccharides	365:393	lipid-linked oligosaccharides	365:393	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	7	theme	donor	396:400	arg1	substrates					402:411	donor substrates	396:411	donor substrates for N-glycosylation of proteins	396:443	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	5	8	theme	transcription	976:988	arg1	repressor					990:998	a general transcription repressor	966:998	a general transcription repressor	966:998	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	1	9	theme	misfolded	269:277	arg1	N-glycoproteins					279:293	misfolded N-glycoproteins	269:293	misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation	269:355	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	0	10	theme	degradation	97:107	arg1	pathway					109:115	an O-glycoprotein degradation pathway	79:115	an O-glycoprotein degradation pathway	79:115	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	3	11	theme	carbon	755:760	arg1	mannose					740:746	mannose	740:746	mannose	740:746	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	3	11	theme	carbon	755:760	arg1	source					762:767	the carbon source	751:767	the carbon source	751:767	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	0	12	attach	derived	13:19	arg1	glycoproteins					41:53	O-mannosylated glycoproteins	26:53	O-mannosylated glycoproteins	26:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	0	12	attach	derived	13:19	arg2	glycans					5:11	Free glycans	0:11	Free glycans derived from O-mannosylated glycoproteins	0:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	5	13	from	integrity	1192:1200	arg1	level					1130:1134	the level	1126:1134	the level of an O-mannosylated protein	1126:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	2	14	theme	other	558:562	arg1	types					564:568	other types	558:568	other types of free glycans	558:584	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	5	15	theme	cyc8	937:940	arg1	gene					942:945	the cyc8 gene	933:945	the cyc8 gene	933:945	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	4	16	theme	O-mannosyl	866:875	arg1	glycans					877:883	O-mannosyl glycans	866:883	O-mannosyl glycans that are attached to glycoproteins	866:918	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	0	17	gly	O-glycoprotein	82:95	arg1	O-glycoprotein					82:95	an O-glycoprotein degradation pathway	79:115	an O-glycoprotein degradation pathway	79:115	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	5	18	gly	O-mannosylated	1142:1155	arg1	protein					1157:1163	an O-mannosylated protein	1139:1163	an O-mannosylated protein	1139:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	6	19	gly	O-glycosylated	1398:1411	arg1	proteins					1413:1420	O-glycosylated proteins	1398:1420	O-glycosylated proteins	1398:1420	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	6	20	theme	pathway	1259:1265	arg1	support					1236:1242	support	1236:1242	support of a regulated pathway for the degradation of O-glycoproteins in yeast	1236:1313	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	5	21	with	concomitant	1070:1080	arg1	integrity					1192:1200	compromised cell wall integrity	1170:1200	compromised cell wall integrity	1170:1200	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	21	with	concomitant	1070:1080	arg1	defect					1103:1108	a severe growth defect	1087:1108	a severe growth defect	1087:1108	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	21	with	concomitant	1070:1080	arg1	reduction					1113:1121	a reduction	1111:1121	a reduction in the level of an O-mannosylated protein	1111:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	4	22	gly	glycoproteins	906:918	arg1	glycoproteins					906:918	glycoproteins	906:918	glycoproteins	906:918	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	0	23	theme	Free	0:3	arg1	glycans					5:11	Free glycans	0:11	Free glycans derived from O-mannosylated glycoproteins	0:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	5	24	theme	cell	1182:1185	arg1	integrity					1192:1200	compromised cell wall integrity	1170:1200	compromised cell wall integrity	1170:1200	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	6	25	theme	regulated	1249:1257	arg1	pathway					1259:1265	a regulated pathway	1247:1265	a regulated pathway for the degradation of O-glycoproteins in yeast	1247:1313	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	1	26	gly	N-glycoproteins	279:293	arg1	N-glycoproteins					279:293	misfolded N-glycoproteins	269:293	misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation	269:355	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	2	27	theme	free	494:497	arg1	N-glycans					499:507	free N-glycans	494:507	free N-glycans	494:507	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	5	28	theme	wall	1187:1190	arg1	integrity					1192:1200	compromised cell wall integrity	1170:1200	compromised cell wall integrity	1170:1200	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	6	29	theme	catabolic	1352:1360	arg1	mechanisms					1362:1371	the catabolic mechanisms	1348:1371	the catabolic mechanisms that control the fate of O-glycosylated proteins	1348:1420	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	5	30	theme	general	968:974	arg1	repressor					990:998	a general transcription repressor	966:998	a general transcription repressor	966:998	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	31	from	reduction	1113:1121	arg1	level					1130:1134	the level	1126:1134	the level of an O-mannosylated protein	1126:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	2	32	theme	N-glycans	499:507	arg1	generation					480:489	the generation	476:489	the generation of free N-glycans	476:507	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	1	33	theme	proteins	436:443	arg1	N-glycosylation					417:431	N-glycosylation	417:431	N-glycosylation of proteins	417:443	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	4	34	theme	structural	772:781	arg1	analysis					783:790	A structural analysis	770:790	A structural analysis of these glycans	770:807	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	1	35	link	lipid-linked	365:376	arg1	oligosaccharides					378:393	lipid-linked oligosaccharides	365:393	lipid-linked oligosaccharides	365:393	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	35	link	lipid-linked	365:376	arg1	substrates					402:411	donor substrates	396:411	donor substrates for N-glycosylation of proteins	396:443	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	36	theme	endoplasmic	312:322	arg1	degradation					345:355	the endoplasmic reticulum-associated degradation	308:355	the endoplasmic reticulum-associated degradation	308:355	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	5	37	theme	growth	1096:1101	arg1	defect					1103:1108	a severe growth defect	1087:1108	a severe growth defect	1087:1108	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	38	theme	amounts	1043:1049	arg1	accumulation					1017:1028	the accumulation	1013:1028	the accumulation of excessive amounts of free O-glycans	1013:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	1	39	gly	N-glycosylation	417:431	arg1	proteins					436:443	proteins	436:443	proteins	436:443	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	3	40	dep	free	654:657	arg1	O-mannosylated					660:673	O-mannosylated	660:673	O-mannosylated	660:673	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	0	41	theme	O-mannosylated	26:39	arg1	glycoproteins					41:53	O-mannosylated glycoproteins	26:53	O-mannosylated glycoproteins	26:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	1	42	theme	reticulum-associated	324:343	arg1	degradation					345:355	the endoplasmic reticulum-associated degradation	308:355	the endoplasmic reticulum-associated degradation	308:355	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	2	43	theme	glycans	578:584	arg1	types					564:568	other types	558:568	other types of free glycans	558:584	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	1	44	dep	N-glycans	232:240	arg1	i.e.					222:225	i.e.	222:225	i.e.	222:225	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	0	45	attach	presence	67:74	arg2	pathway					109:115	an O-glycoprotein degradation pathway	79:115	an O-glycoprotein degradation pathway	79:115	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	0	45	attach	presence	67:74	arg1	yeast					120:124	yeast	120:124	yeast	120:124	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	1	46	theme	free	227:230	arg1	N-glycans					232:240	free N-glycans	227:240	free N-glycans	227:240	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	46	theme	free	227:230	arg1	oligosaccharides					161:176	unconjugated oligosaccharides	148:176	unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans)	148:241	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	2	47	theme	free	573:576	arg1	glycans					578:584	free glycans	573:584	free glycans	573:584	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	5	48	from	defect	1103:1108	arg1	level					1130:1134	the level	1126:1134	the level of an O-mannosylated protein	1126:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	0	49	from	presence	67:74	arg1	yeast					120:124	yeast	120:124	yeast	120:124	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	5	50	theme	free	1054:1057	arg1	O-glycans					1059:1067	free O-glycans	1054:1067	free O-glycans	1054:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	51	theme	gene	942:945	arg1	Deletion					921:928	Deletion	921:928	Deletion of the cyc8 gene, which encodes for a general transcription repressor,	921:999	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	52	theme	O-mannosylated	1142:1155	arg1	protein					1157:1163	an O-mannosylated protein	1139:1163	an O-mannosylated protein	1139:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	3	53	theme	glycans	675:681	arg1	accumulation					638:649	the accumulation	634:649	the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source	634:767	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	5	54	theme	O-glycans	1059:1067	arg1	amounts					1043:1049	excessive amounts	1033:1049	excessive amounts of free O-glycans	1033:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	54	theme	O-glycans	1059:1067	arg1	O-glycans					1059:1067	free O-glycans	1054:1067	free O-glycans	1054:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	55	theme	protein	1157:1163	arg1	level					1130:1134	the level	1126:1134	the level of an O-mannosylated protein	1126:1163	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	4	56	attach	attached	894:901	arg1	glycoproteins					906:918	glycoproteins	906:918	glycoproteins	906:918	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	4	56	attach	attached	894:901	arg2	glycans					877:883	O-mannosyl glycans	866:883	O-mannosyl glycans that are attached to glycoproteins	866:918	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	5	57	theme	compromised	1170:1180	arg1	integrity					1192:1200	compromised cell wall integrity	1170:1200	compromised cell wall integrity	1170:1200	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	3	58	theme	free	654:657	arg1	glycans					675:681	free, O-mannosylated glycans	654:681	free, O-mannosylated glycans	654:681	Here, we report on the accumulation of free, O-mannosylated glycans in budding yeast that were cultured in medium containing mannose as the carbon source.					
31311856	0	59	gly	O-mannosylated	26:39	arg1	glycoproteins					41:53	O-mannosylated glycoproteins	26:53	O-mannosylated glycoproteins	26:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	1	60	theme	eukaryotic	130:139	arg1	cells					141:145	eukaryotic cells	130:145	eukaryotic cells	130:145	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	4	61	theme	glycans	801:807	arg1	analysis					783:790	A structural analysis	770:790	A structural analysis of these glycans	770:807	A structural analysis of these glycans revealed that their structures are identical to those of O-mannosyl glycans that are attached to glycoproteins.					
31311856	0	62	gly	glycoproteins	41:53	arg1	glycoproteins					41:53	O-mannosylated glycoproteins	26:53	O-mannosylated glycoproteins	26:53	Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast.					
31311856	6	63	from	degradation	1275:1285	arg1	yeast					1309:1313	yeast	1309:1313	yeast	1309:1313	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	6	64	theme	proteins	1413:1420	arg1	fate					1390:1393	the fate	1386:1393	the fate of O-glycosylated proteins	1386:1420	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	2	65	theme	responsible	460:470	arg1	well-understood					516:530	well-understood	516:530	well-understood	516:530	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	2	65	theme	responsible	460:470	arg1	mechanism					450:458	The mechanism	446:458	The mechanism responsible for the generation of free N-glycans	446:507	The mechanism responsible for the generation of free N-glycans is now well-understood, but the issue of whether other types of free glycans are present remains unclear.					
31311856	6	66	gly	O-glycoproteins	1290:1304	arg1	O-glycoproteins					1290:1304	O-glycoproteins	1290:1304	O-glycoproteins	1290:1304	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	6	67	theme	O-glycosylated	1398:1411	arg1	proteins					1413:1420	O-glycosylated proteins	1398:1420	O-glycosylated proteins	1398:1420	Our findings provide evidence in support of a regulated pathway for the degradation of O-glycoproteins in yeast and offer critical insights into the catabolic mechanisms that control the fate of O-glycosylated proteins.					
31311856	1	68	theme	lipid-linked	365:376	arg1	oligosaccharides					378:393	lipid-linked oligosaccharides	365:393	lipid-linked oligosaccharides	365:393	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	68	theme	lipid-linked	365:376	arg1	substrates					402:411	donor substrates	396:411	donor substrates for N-glycosylation of proteins	396:443	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	5	69	theme	excessive	1033:1041	arg1	amounts					1043:1049	excessive amounts	1033:1049	excessive amounts of free O-glycans	1033:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	5	69	theme	excessive	1033:1041	arg1	O-glycans					1059:1067	free O-glycans	1054:1067	free O-glycans	1054:1067	Deletion of the cyc8 gene, which encodes for a general transcription repressor, resulted in the accumulation of excessive amounts of free O-glycans, concomitant with a severe growth defect, a reduction in the level of an O-mannosylated protein, and compromised cell wall integrity.					
31311856	1	70	theme	unconjugated	148:159	arg1	N-glycans					232:240	free N-glycans	227:240	free N-glycans	227:240	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31311856	1	70	theme	unconjugated	148:159	arg1	oligosaccharides					161:176	unconjugated oligosaccharides	148:176	unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans)	148:241	In eukaryotic cells, unconjugated oligosaccharides that are structurally related to N-glycans (i.e. free N-glycans) are generated either from misfolded N-glycoproteins destined for the endoplasmic reticulum-associated degradation or from lipid-linked oligosaccharides, donor substrates for N-glycosylation of proteins.					
31494161	3	0	theme	MMT	686:688	arg1	nanoclay					690:697	the reinforced MMT nanoclay	671:697	the reinforced MMT nanoclay	671:697	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	8	1	from	interaction	1199:1209	arg1	multicomponent					1235:1248	multicomponent	1235:1248	multicomponent	1235:1248	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	5	2	theme	composite	859:867	arg1	solution					869:876	The AGM composite solution	851:876	The AGM composite solution	851:876	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	0	3	theme	agar/gellan	72:82	arg1	properties					58:67	Rheological, morphological, mechanical, and water-barrier properties	0:67	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan	0:82	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	8	4	from	structure	1178:1186	arg1	multicomponent					1235:1248	multicomponent	1235:1248	multicomponent	1235:1248	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	4	5	theme	rheological	818:828	arg1	properties					830:839	the rheological properties	814:839	the rheological properties	814:839	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	10	6	theme	new	1420:1422	arg1	route					1424:1428	a new route	1418:1428	a new route for enhancing the nanocomposites for sustainable materials	1418:1487	The results of this study might introduce a new route for enhancing the nanocomposites for sustainable materials.					
31494161	0	7	theme	composite	109:117	arg1	films					119:123	clay composite films	104:123	clay composite films	104:123	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	8	8	theme	nanocomposite	1164:1176	arg1	structure					1178:1186	The nanocomposite structure	1160:1186	The nanocomposite structure	1160:1186	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	4	9	theme	contact	732:738	arg1	56.8°-49.4°					747:757	56.8°-49.4°	747:757	56.8°-49.4°	747:757	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	4	9	theme	contact	732:738	arg1	angle					740:744	contact angle	732:744	contact angle (56.8°-49.4°)	732:758	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	0	10	theme	clay	104:107	arg1	films					119:123	clay composite films	104:123	clay composite films	104:123	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	8	11	from	interaction	1216:1226	arg1	multicomponent					1235:1248	multicomponent	1235:1248	multicomponent	1235:1248	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	7	12	theme	shear	1098:1102	arg1	viscosity					1104:1112	the shear viscosity	1094:1112	the shear viscosity	1094:1112	The complex viscosity differed from the shear viscosity and remained higher than the shear viscosity.					
31494161	5	13	theme	high	941:944	arg1	rate					946:949	a high rate	939:949	a high rate	939:949	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	9	14	theme	reinforcement	1354:1366	arg1	effect					1368:1373	a synergistic reinforcement effect	1340:1373	a synergistic reinforcement effect	1340:1373	The AG and AGM nanocomposites exhibited a synergistic reinforcement effect.					
31494161	1	15	theme	solution-casting	231:246	arg1	method					248:253	the solution-casting method	227:253	the solution-casting method for food packaging applications	227:285	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	4	16	theme	clay	801:804	arg1	incorporation					780:792	the incorporation	776:792	the incorporation of MMT clay	776:804	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	8	17	theme	XRD	1278:1280	arg1	analysis					1290:1297	FT-IR, XRD and SEM analysis	1271:1297	FT-IR, XRD and SEM analysis	1271:1297	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	1	18	dep	Agar	126:129	arg1	films					203:207	based ternary nanocomposite films	175:207	based ternary nanocomposite films	175:207	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	5	19	theme	thinning	894:901	arg1	behavior					903:910	shear thinning behavior	888:910	shear thinning behavior	888:910	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	2	20	theme	structure-property	368:385	arg1	relationships					387:399	the structure-property relationships	364:399	the structure-property relationships	364:399	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	9	21	theme	AG	1304:1305	arg1	nanocomposites					1315:1328	The AG and AGM nanocomposites	1300:1328	The AG and AGM nanocomposites	1300:1328	The AG and AGM nanocomposites exhibited a synergistic reinforcement effect.					
31494161	4	22	theme	MMT	797:799	arg1	clay					801:804	MMT clay	797:804	MMT clay	797:804	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	2	23	from	effect	342:347	arg1	relationships					387:399	the structure-property relationships	364:399	the structure-property relationships	364:399	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	2	24	theme	rheological	434:444	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	2	25	theme	thermal	459:465	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	2	26	theme	ultraviolet	468:478	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	7	27	theme	complex	1062:1068	arg1	viscosity					1070:1078	The complex viscosity	1058:1078	The complex viscosity	1058:1078	The complex viscosity differed from the shear viscosity and remained higher than the shear viscosity.					
31494161	2	28	theme	prepared	292:299	arg1	nanocomposites					301:314	The prepared nanocomposites	288:314	The prepared nanocomposites	288:314	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	6	29	theme	storage	1012:1018	arg1	modulus					1029:1035	significantly higher storage and loss modulus	991:1035	significantly higher storage and loss modulus	991:1035	Additionally, the composites exhibited significantly higher storage and loss modulus at high frequencies.					
31494161	0	30	theme	Rheological	0:10	arg1	properties					58:67	Rheological, morphological, mechanical, and water-barrier properties	0:67	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan	0:82	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	9	31	theme	AGM	1311:1313	arg1	nanocomposites					1315:1328	The AG and AGM nanocomposites	1300:1328	The AG and AGM nanocomposites	1300:1328	The AG and AGM nanocomposites exhibited a synergistic reinforcement effect.					
31494161	3	32	theme	thermal	568:574	arg1	stability					576:584	the thermal stability	564:584	the thermal stability (T5%: 119.4-174.7)	564:603	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	2	33	theme	clay	356:359	arg1	effect					342:347	the effect	338:347	the effect of MMT clay on the structure-property relationships	338:399	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	7	34	theme	shear	1143:1147	arg1	viscosity					1149:1157	the shear viscosity	1139:1157	the shear viscosity	1139:1157	The complex viscosity differed from the shear viscosity and remained higher than the shear viscosity.					
31494161	8	35	theme	SEM	1286:1288	arg1	analysis					1290:1297	FT-IR, XRD and SEM analysis	1271:1297	FT-IR, XRD and SEM analysis	1271:1297	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	3	36	dep	stability	576:584	arg1	%					589:589	T5%	587:589	T5%: 119.4-174.7	587:602	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	2	37	theme	microstructural	417:431	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	1	38	theme	food	259:262	arg1	applications					274:285	food packaging applications	259:285	food packaging applications	259:285	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	5	39	theme	viscosity	916:924	arg1	reduction					926:934	viscosity reduction	916:934	viscosity reduction	916:934	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	1	40	theme	packaging	264:272	arg1	applications					274:285	food packaging applications	259:285	food packaging applications	259:285	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	3	41	theme	T5	587:588	arg1	%					589:589	T5%	587:589	T5%: 119.4-174.7	587:602	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	3	42	dep	%	589:589	arg1	119.4-174.7					592:602	119.4-174.7	592:602	119.4-174.7	592:602	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	6	43	theme	loss	1024:1027	arg1	modulus					1029:1035	significantly higher storage and loss modulus	991:1035	significantly higher storage and loss modulus	991:1035	Additionally, the composites exhibited significantly higher storage and loss modulus at high frequencies.					
31494161	0	44	theme	mechanical	28:37	arg1	properties					58:67	Rheological, morphological, mechanical, and water-barrier properties	0:67	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan	0:82	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	9	45	theme	synergistic	1342:1352	arg1	effect					1368:1373	a synergistic reinforcement effect	1340:1373	a synergistic reinforcement effect	1340:1373	The AG and AGM nanocomposites exhibited a synergistic reinforcement effect.					
31494161	2	46	theme	composites	525:534	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	3	47	theme	tensile	609:615	arg1	29.9-44 MPa					627:637	29.9-44 MPa	627:637	29.9-44 MPa	627:637	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	3	47	theme	tensile	609:615	arg1	strength					617:624	tensile strength	609:624	tensile strength (29.9-44 MPa)	609:638	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	8	48	theme	molecular	1189:1197	arg1	interaction					1199:1209	molecular interaction	1189:1209	molecular interaction	1189:1209	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	1	49	theme	based	175:179	arg1	films					203:207	based ternary nanocomposite films	175:207	based ternary nanocomposite films	175:207	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	0	50	theme	water-barrier	44:56	arg1	properties					58:67	Rheological, morphological, mechanical, and water-barrier properties	0:67	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan	0:82	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	2	51	theme	hydrogel	516:523	arg1	composites					525:534	AG hydrogel composites	513:534	AG hydrogel composites	513:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	2	52	theme	mechanical	447:456	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	5	53	theme	shear	888:892	arg1	behavior					903:910	shear thinning behavior	888:910	shear thinning behavior	888:910	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	10	54	theme	study	1396:1400	arg1	results					1380:1386	The results	1376:1386	The results of this study	1376:1400	The results of this study might introduce a new route for enhancing the nanocomposites for sustainable materials.					
31494161	6	55	theme	higher	1005:1010	arg1	modulus					1029:1035	significantly higher storage and loss modulus	991:1035	significantly higher storage and loss modulus	991:1035	Additionally, the composites exhibited significantly higher storage and loss modulus at high frequencies.					
31494161	4	56	theme	water	704:708	arg1	1.9-1.7					719:725	1.9-1.7	719:725	1.9-1.7	719:725	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	4	56	theme	water	704:708	arg1	barrier					710:716	The water barrier	700:716	The water barrier (1.9-1.7)	700:726	The water barrier (1.9-1.7) and contact angle (56.8°-49.4°) were reduced by the incorporation of MMT clay whereas the rheological properties improved.					
31494161	1	57	theme	ternary	181:187	arg1	films					203:207	based ternary nanocomposite films	175:207	based ternary nanocomposite films	175:207	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	6	58	theme	high	1040:1043	arg1	frequencies					1045:1055	high frequencies	1040:1055	high frequencies	1040:1055	Additionally, the composites exhibited significantly higher storage and loss modulus at high frequencies.					
31494161	10	59	theme	sustainable	1467:1477	arg1	materials					1479:1487	sustainable materials	1467:1487	sustainable materials	1467:1487	The results of this study might introduce a new route for enhancing the nanocomposites for sustainable materials.					
31494161	5	60	theme	AGM	855:857	arg1	solution					869:876	The AGM composite solution	851:876	The AGM composite solution	851:876	The AGM composite solution exhibited shear thinning behavior and viscosity reduction at a high rate.					
31494161	1	61	theme	nanocomposite	189:201	arg1	films					203:207	based ternary nanocomposite films	175:207	based ternary nanocomposite films	175:207	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	2	62	theme	water-barrier	485:497	arg1	properties					499:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties	413:508	the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites	413:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	8	63	theme	FT-IR	1271:1275	arg1	analysis					1290:1297	FT-IR, XRD and SEM analysis	1271:1297	FT-IR, XRD and SEM analysis	1271:1297	The nanocomposite structure, molecular interaction, and interaction in the multicomponent were investigated by FT-IR, XRD and SEM analysis.					
31494161	2	64	theme	MMT	352:354	arg1	clay					356:359	MMT clay	352:359	MMT clay	352:359	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
31494161	3	65	theme	reinforced	675:684	arg1	nanoclay					690:697	the reinforced MMT nanoclay	671:697	the reinforced MMT nanoclay	671:697	The results indicated that the thermal stability (T5%: 119.4-174.7) and tensile strength (29.9-44 MPa) were significantly enhanced in the reinforced MMT nanoclay.					
31494161	0	66	theme	morphological	13:25	arg1	properties					58:67	Rheological, morphological, mechanical, and water-barrier properties	0:67	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan	0:82	Rheological, morphological, mechanical, and water-barrier properties of agar/gellan gum/montmorillonite clay composite films.					
31494161	1	67	theme	gellan	136:141	arg1	G					148:148	G	148:148	G	148:148	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	1	67	theme	gellan	136:141	arg1	gum					143:145	gellan gum	136:145	gellan gum (G)	136:149	Agar (A), gellan gum (G) and montmorillonite (M) based ternary nanocomposite films were prepared via the solution-casting method for food packaging applications.					
31494161	2	68	theme	AG	513:514	arg1	composites					525:534	AG hydrogel composites	513:534	AG hydrogel composites	513:534	The prepared nanocomposites were investigated for the effect of MMT clay on the structure-property relationships by studying the microstructural, rheological, mechanical, thermal, ultraviolet, and water-barrier properties of AG hydrogel composites.					
28921428	1	0	theme	natural	136:142	arg1	counterpart					144:154	their natural counterpart	130:154	their natural counterpart	130:154	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	4	1	theme	protein	791:797	arg1	quantities					761:770	high quantities	756:770	high quantities of the recombinant protein	756:797	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	2	2	theme	product	499:505	arg1	efficacy					471:478	the efficacy	467:478	the efficacy of the recombinant product	467:505	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	0	3	from	Production	0:9	arg1	Lines					52:56	Human Cell Lines	41:56	Human Cell Lines	41:56	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	4	4	theme	high	756:759	arg1	quantities					761:770	high quantities	756:770	high quantities of the recombinant protein	756:797	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	4	5	theme	cell	701:704	arg1	line					706:709	the human cell line SK-HEP	691:716	the human cell line SK-HEP	691:716	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	2	6	theme	recombinant	487:497	arg1	product					499:505	the recombinant product	483:505	the recombinant product	483:505	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	4	7	theme	human	695:699	arg1	line					706:709	the human cell line SK-HEP	691:716	the human cell line SK-HEP	691:716	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	2	8	theme	Galα1,3-Gal	338:348	arg1	residues					401:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	1	9	theme	similar	119:125	arg1	proteins					100:107	recombinant proteins	88:107	recombinant proteins much more similar to their natural counterpart	88:154	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	1	10	theme	Human	59:63	arg1	lines					70:74	Human cell lines	59:74	Human cell lines	59:74	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	2	11	theme	acid	387:390	arg1	residues					401:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	1	12	theme	cell	65:68	arg1	lines					70:74	Human cell lines	59:74	Human cell lines	59:74	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	2	13	theme	N-glycolylneuraminic	366:385	arg1	Neu5Gc					393:398	Neu5Gc	393:398	Neu5Gc	393:398	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	2	13	theme	N-glycolylneuraminic	366:385	arg1	acid					387:390	N-glycolylneuraminic acid	366:390	N-glycolylneuraminic acid (Neu5Gc)	366:399	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	4	14	theme	recombinant	779:789	arg1	protein					791:797	the recombinant protein	775:797	the recombinant protein	775:797	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	2	15	theme	structure	303:311	arg1	epitopes					320:327	its structure glycan epitopes	299:327	its structure glycan epitopes	299:327	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	2	15	theme	structure	303:311	arg1	residues					401:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	3	16	theme	factor	549:554	arg1	rFVIII					562:567	rFVIII	562:567	rFVIII	562:567	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	16	theme	factor	549:554	arg1	VIII					556:559	recombinant factor VIII	537:559	recombinant factor VIII (rFVIII)	537:568	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	1	17	theme	other	169:173	arg1	lines					190:194	other mammalian cell lines	169:194	other mammalian cell lines	169:194	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	0	18	theme	Factor	26:31	arg1	VIII					33:36	Recombinant Factor VIII	14:36	Recombinant Factor VIII	14:36	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	4	19	theme	lentiviral	727:736	arg1	vector					738:743	a lentiviral vector	725:743	a lentiviral vector to produce high quantities of the recombinant protein	725:797	Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.					
28921428	1	20	theme	mammalian	175:183	arg1	lines					190:194	other mammalian cell lines	169:194	other mammalian cell lines	169:194	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	0	21	theme	Recombinant	14:24	arg1	VIII					33:36	Recombinant Factor VIII	14:36	Recombinant Factor VIII	14:36	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	2	22	theme	nonhuman	272:279	arg1	cells					281:285	nonhuman cells	272:285	nonhuman cells	272:285	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	3	23	theme	new	595:597	arg1	approach					599:606	a new approach	593:606	a new approach to avoid nonhuman glycosylation	593:638	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	23	theme	new	595:597	arg1	production					523:532	the production	519:532	the production of recombinant factor VIII (rFVIII) in human cell lines	519:588	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	1	24	theme	recombinant	88:98	arg1	proteins					100:107	recombinant proteins	88:107	recombinant proteins much more similar to their natural counterpart	88:154	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	1	25	theme	cell	185:188	arg1	lines					190:194	other mammalian cell lines	169:194	other mammalian cell lines	169:194	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	0	26	theme	VIII	33:36	arg1	Production					0:9	Production	0:9	Production of Recombinant Factor VIII in Human Cell Lines.	0:57	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	0	27	theme	Cell	47:50	arg1	Lines					52:56	Human Cell Lines	41:56	Human Cell Lines	41:56	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	3	28	theme	VIII	556:559	arg1	approach					599:606	a new approach	593:606	a new approach to avoid nonhuman glycosylation	593:638	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	28	theme	VIII	556:559	arg1	production					523:532	the production	519:532	the production of recombinant factor VIII (rFVIII) in human cell lines	519:588	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	0	29	theme	Human	41:45	arg1	Lines					52:56	Human Cell Lines	41:56	Human Cell Lines	41:56	Production of Recombinant Factor VIII in Human Cell Lines.					
28921428	3	30	theme	nonhuman	617:624	arg1	glycosylation					626:638	nonhuman glycosylation	617:638	nonhuman glycosylation	617:638	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	31	from	production	523:532	arg1	lines					584:588	human cell lines	573:588	human cell lines	573:588	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	32	theme	recombinant	537:547	arg1	rFVIII					562:567	rFVIII	562:567	rFVIII	562:567	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	32	theme	recombinant	537:547	arg1	VIII					556:559	recombinant factor VIII	537:559	recombinant factor VIII (rFVIII)	537:568	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	3	33	theme	cell	579:582	arg1	lines					584:588	human cell lines	573:588	human cell lines	573:588	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
28921428	1	34	dep	lines	190:194	arg1	reducing					197:204	reducing	197:204	reducing potential immunogenic reactions	197:236	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	2	35	theme	glycan	313:318	arg1	epitopes					320:327	its structure glycan epitopes	299:327	its structure glycan epitopes	299:327	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	2	35	theme	glycan	313:318	arg1	residues					401:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues	338:408	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	1	36	theme	potential	206:214	arg1	reactions					228:236	potential immunogenic reactions	206:236	potential immunogenic reactions	206:236	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	2	37	theme	Recombinant	239:249	arg1	proteins					251:258	Recombinant proteins	239:258	Recombinant proteins produced in nonhuman cells	239:285	Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product.					
28921428	1	38	theme	immunogenic	216:226	arg1	reactions					228:236	potential immunogenic reactions	206:236	potential immunogenic reactions	206:236	Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions.					
28921428	3	39	theme	human	573:577	arg1	lines					584:588	human cell lines	573:588	human cell lines	573:588	Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation.					
30685086	1	0	theme	mucin-type	198:207	arg1	O-glycosylation					209:223	mucin-type O-glycosylation	198:223	mucin-type O-glycosylation	198:223	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	1	theme	Thr	301:303	arg1	residues					305:312	Ser or Thr residues	294:312	Ser or Thr residues of target proteins	294:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	1	theme	Thr	301:303	arg1	proteins					324:331	target proteins	317:331	target proteins	317:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	2	2	theme	capable	394:400	arg1	GalNAc-T7					372:380	GalNAc-T7	372:380	GalNAc-T7	372:380	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	2	2	theme	capable	394:400	arg1	GalNAc-T					385:392	a GalNAc-T	383:392	a GalNAc-T capable of glycosylating consecutive sites	383:435	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	2	3	with	complex	449:455	arg1	UDP-GalNAc					482:491	the donor substrate UDP-GalNAc	462:491	the donor substrate UDP-GalNAc	462:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	1	4	theme	target	317:322	arg1	proteins					324:331	target proteins	317:331	target proteins	317:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	5	5	theme	significant	861:871	arg1	changes					888:894	significant conformational changes	861:894	significant conformational changes	861:894	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	3	6	theme	lectin	541:546	arg1	domain					548:553	C-terminal lectin domain	530:553	C-terminal lectin domain	530:553	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	3	7	theme	flexible	574:581	arg1	linker					583:588	a flexible linker	572:588	a flexible linker	572:588	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	5	8	theme	catalytic	929:937	arg1	reaction					939:946	the catalytic reaction	925:946	the catalytic reaction	925:946	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	5	9	theme	catalytic	824:832	arg1	center					834:839	the catalytic center	820:839	the catalytic center of the complex	820:854	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	4	10	theme	glycosyl	697:704	arg1	group					706:710	the glycosyl group	693:710	the glycosyl group	693:710	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	0	11	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.	0:113	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	1	12	theme	proteins	324:331	arg1	residues					305:312	Ser or Thr residues	294:312	Ser or Thr residues of target proteins	294:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	12	theme	proteins	324:331	arg1	proteins					324:331	target proteins	317:331	target proteins	317:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	2	13	theme	sites	431:435	arg1	capable					394:400	capable	394:400	capable	394:400	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	2	14	theme	substrate	472:480	arg1	UDP-GalNAc					482:491	the donor substrate UDP-GalNAc	462:491	the donor substrate UDP-GalNAc	462:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	2	15	gly	glycosylating	405:417	arg0	sites					431:435	glycosylating consecutive sites	405:435	glycosylating consecutive sites	405:435	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	2	16	theme	crystal	350:356	arg1	structures					358:367	the crystal structures	346:367	the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc	346:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	3	17	theme	catalytic	509:517	arg1	domain					519:524	The N-terminal catalytic domain	494:524	The N-terminal catalytic domain	494:524	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	2	18	theme	donor	466:470	arg1	UDP-GalNAc					482:491	the donor substrate UDP-GalNAc	462:491	the donor substrate UDP-GalNAc	462:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	3	19	theme	narrow	601:606	arg1	cleft					608:612	a narrow cleft	599:612	a narrow cleft for the acceptor substrate	599:639	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	0	20	theme	activity	42:49	arg1	basis					11:15	Structural basis	0:15	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.	0:113	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	4	21	theme	domain	677:682	arg1	domain					677:682	the lectin domain	666:682	the lectin domain	666:682	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	4	21	theme	domain	677:682	arg1	subdomain					653:661	Only the α subdomain	642:661	Only the α subdomain of the lectin domain	642:682	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	4	22	theme	lectin	670:675	arg1	domain					677:682	the lectin domain	666:682	the lectin domain	666:682	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	2	23	theme	complex	449:455	arg1	structures					358:367	the crystal structures	346:367	the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc	346:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	0	24	theme	transfer	33:40	arg1	activity					42:49	carbohydrate transfer activity	20:49	carbohydrate transfer activity of UDP-GalNAc	20:63	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	0	25	theme	carbohydrate	20:31	arg1	activity					42:49	carbohydrate transfer activity	20:49	carbohydrate transfer activity of UDP-GalNAc	20:63	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	5	26	theme	conformational	873:886	arg1	changes					888:894	significant conformational changes	861:894	significant conformational changes	861:894	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	1	27	theme	UDP-	279:282	arg1	GalNAc					284:289	UDP- GalNAc	279:289	UDP- GalNAc	279:289	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	0	28	theme	UDP-GalNAc	54:63	arg1	activity					42:49	carbohydrate transfer activity	20:49	carbohydrate transfer activity of UDP-GalNAc	20:63	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	1	29	theme	polypeptide	130:140	arg1	GalNAc-Ts					178:186	GalNAc-Ts	178:186	GalNAc-Ts	178:186	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	29	theme	polypeptide	130:140	arg1	N-acetylgalactosaminyltransferases					142:175	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	115:175	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts)	115:187	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	3	30	theme	acceptor	622:629	arg1	substrate					631:639	the acceptor substrate	618:639	the acceptor substrate	618:639	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	4	31	theme	key	729:731	arg1	residues					733:740	key residues	729:740	key residues	729:740	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	4	32	theme	substrate	752:760	arg1	binding					762:768	substrate binding	752:768	substrate binding	752:768	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	1	33	theme	UDP-GalNAc	119:128	arg1	GalNAc-Ts					178:186	GalNAc-Ts	178:186	GalNAc-Ts	178:186	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	33	theme	UDP-GalNAc	119:128	arg1	N-acetylgalactosaminyltransferases					142:175	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	115:175	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts)	115:187	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	5	34	theme	reaction	939:946	arg1	mechanism					912:920	the mechanism	908:920	the mechanism of the catalytic reaction	908:946	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	2	35	theme	GalNAc-T7	372:380	arg1	structures					358:367	the crystal structures	346:367	the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc	346:491	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	0	36	theme	Polypeptide	66:76	arg1	N-acetylgalactosaminyltransferase					78:110	Polypeptide N-acetylgalactosaminyltransferase 7	66:112	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.	0:113	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	3	37	theme	N-terminal	498:507	arg1	domain					519:524	The N-terminal catalytic domain	494:524	The N-terminal catalytic domain	494:524	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	0	38	dep	basis	11:15	arg1	N-acetylgalactosaminyltransferase					78:110	Polypeptide N-acetylgalactosaminyltransferase 7	66:112	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.	0:113	Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7.					
30685086	5	39	theme	complex	848:854	arg1	center					834:839	the catalytic center	820:839	the catalytic center of the complex	820:854	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	2	40	theme	consecutive	419:429	arg1	sites					431:435	glycosylating consecutive sites	405:435	glycosylating consecutive sites	405:435	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	3	41	theme	C-terminal	530:539	arg1	domain					548:553	C-terminal lectin domain	530:553	C-terminal lectin domain	530:553	The N-terminal catalytic domain and C-terminal lectin domain are connected by a flexible linker, forming a narrow cleft for the acceptor substrate.					
30685086	1	42	theme	Ser	294:296	arg1	residues					305:312	Ser or Thr residues	294:312	Ser or Thr residues of target proteins	294:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	1	42	theme	Ser	294:296	arg1	proteins					324:331	target proteins	317:331	target proteins	317:331	The UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) catalyze mucin-type O-glycosylation by transferring α-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to Ser or Thr residues of target proteins.					
30685086	5	43	theme	Apo	787:789	arg1	structure					791:799	the Apo structure	783:799	the Apo structure	783:799	Compared to the Apo structure, the loop covering the catalytic center of the complex show significant conformational changes, indicating the mechanism of the catalytic reaction.					
30685086	2	44	theme	glycosylating	405:417	arg1	sites					431:435	glycosylating consecutive sites	405:435	glycosylating consecutive sites	405:435	We resolved the crystal structures of GalNAc-T7, a GalNAc-T capable of glycosylating consecutive sites, and of its complex with the donor substrate UDP-GalNAc.					
30685086	4	45	theme	α	651:651	arg1	domain					677:682	the lectin domain	666:682	the lectin domain	666:682	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
30685086	4	45	theme	α	651:651	arg1	subdomain					653:661	Only the α subdomain	642:661	Only the α subdomain of the lectin domain	642:682	Only the α subdomain of the lectin domain binds to the glycosyl group, indicating that key residues determine substrate binding.					
31169903	8	0	theme	older	1153:1157	arg1	children					1159:1166	older children	1153:1166	older children	1153:1166	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	8	1	from	RESULTS	1015:1021	arg1	children					1026:1033	children	1026:1033	children up to 18 months of age, most TSH molecules	1026:1076	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	9	2	dep	months	1262:1267	arg1	up					1254:1255	up	1254:1255	up	1254:1255	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	13	3	theme	sulfonated	1821:1830	arg1	molecules					1836:1844	the highly sulfonated TSH molecules	1810:1844	the highly sulfonated TSH molecules in serum samples of infants	1810:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	5	4	theme	MAIN	662:665	arg1	DESIGN					640:645	DESIGN	640:645	DESIGN	640:645	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	5	4	theme	MAIN	662:665	arg1	MEASURES					675:682	MAIN OUTCOME MEASURES	662:682	MAIN OUTCOME MEASURES	662:682	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	6	5	theme	di-glycosylated	797:811	arg1	TSH					813:815	di-glycosylated TSH	797:815	di-glycosylated TSH	797:815	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	10	6	dep	months	1373:1378	arg1	up					1360:1361	up	1360:1361	up	1360:1361	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	1	7	theme	different	234:242	arg1	situations					271:280	different physiological and clinical situations	234:280	different physiological and clinical situations	234:280	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	9	8	from	extracts	1245:1252	arg1	similar					1214:1220	similar	1214:1220	similar	1214:1220	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	9	8	from	extracts	1245:1252	arg1	degree					1184:1189	The degree	1180:1189	The degree of N-glycosylation	1180:1208	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	12	9	from	thyroid	1650:1656	arg1	biopotency					1636:1645	a high biopotency	1629:1645	a high biopotency at thyroid and extrathyroidal TSH receptors	1629:1689	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	4	10	theme	TSH	607:609	arg1	molecules					611:619	circulating TSH molecules	595:619	circulating TSH molecules	595:619	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	10	11	theme	age	1366:1368	arg1	months					1373:1378	age 18 months	1366:1378	age 18 months	1366:1378	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	5	12	theme	DESIGN	640:645	arg1	samples					690:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples	640:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples	640:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	3	13	from	compositions	461:472	arg1	children					483:490	young children	477:490	young children	477:490	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	13	14	theme	function	1762:1769	arg1	explanation					1795:1805	a likely explanation	1786:1805	a likely explanation of the highly sulfonated TSH molecules in serum samples of infants	1786:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	14	theme	function	1762:1769	arg1	development					1702:1712	A delayed development	1692:1712	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth	1692:1781	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	10	15	theme	low-sialylated	1384:1397	arg1	molecules					1403:1411	low-sialylated TSH molecules	1384:1411	low-sialylated TSH molecules	1384:1411	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	2	16	theme	sialylation	297:307	arg1	degree					287:292	The degree	283:292	The degree of sialylation of serum TSH	283:320	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	2	16	theme	sialylation	297:307	arg1	low					346:348	low	346:348	low	346:348	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	1	17	theme	human	169:173	arg1	molecules					179:187	human TSH molecules	169:187	human TSH molecules	169:187	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	5	18	theme	euthyroid	717:725	arg1	individuals					727:737	euthyroid individuals	717:737	euthyroid individuals	717:737	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	1	19	theme	molecules	179:187	arg1	N-glycosylation					127:141	CONTEXT N-glycosylation	119:141	CONTEXT N-glycosylation	119:141	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	1	19	theme	molecules	179:187	arg1	composition					154:164	glycan composition	147:164	glycan composition	147:164	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	4	20	from	N-glycosylation	553:567	arg1	children					630:637	young children	624:637	young children	624:637	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	6	21	gly	di-glycosylated	797:811	arg1	TSH					813:815	di-glycosylated TSH	797:815	di-glycosylated TSH	797:815	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	13	22	theme	SO3-N-acetylgalactosamine	1727:1751	arg1	function					1762:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	12	23	from	receptors	1681:1689	arg1	biopotency					1636:1645	a high biopotency	1629:1645	a high biopotency at thyroid and extrathyroidal TSH receptors	1629:1689	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	1	24	gly	N-glycosylation	127:141	arg1	molecules					179:187	human TSH molecules	169:187	human TSH molecules	169:187	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	11	25	theme	Most	1469:1472	arg1	molecules					1478:1486	Most TSH molecules	1469:1486	Most TSH molecules in young children	1469:1504	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	0	26	theme	Sulfonation	52:62	arg1	Pattern					7:13	Unique Pattern	0:13	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children	0:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	9	27	theme	age	1272:1274	arg1	months					1262:1267	3 months	1260:1267	3 months of age and after that was higher in serum than in pituitary extracts	1260:1336	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	0	28	dep	18	108:109	arg1	to					105:106	to	105:106	to	105:106	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	12	29	theme	extrathyroidal	1662:1675	arg1	receptors					1681:1689	extrathyroidal TSH receptors	1662:1689	extrathyroidal TSH receptors	1662:1689	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	0	30	theme	TSH	67:69	arg1	Molecules					71:79	TSH Molecules	67:79	TSH Molecules in Serum of Children	67:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	13	31	theme	delayed	1694:1700	arg1	explanation					1795:1805	a likely explanation	1786:1805	a likely explanation of the highly sulfonated TSH molecules in serum samples of infants	1786:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	31	theme	delayed	1694:1700	arg1	development					1702:1712	A delayed development	1692:1712	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth	1692:1781	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	10	32	theme	similar	1414:1420	arg1	molecules					1403:1411	low-sialylated TSH molecules	1384:1411	low-sialylated TSH molecules	1384:1411	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	11	33	theme	young	1491:1495	arg1	children					1497:1504	young children	1491:1504	young children	1491:1504	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	3	34	theme	glycan	454:459	arg1	compositions					461:472	their glycan compositions	448:472	their glycan compositions	448:472	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	3	35	theme	circulating	417:427	arg1	glycoforms					433:442	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	5	36	theme	age	753:755	arg1	weeks					759:763	age 2 weeks	753:763	age 2 weeks to 3 years	753:774	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	5	36	theme	age	753:755	arg1	children					743:750	33 children	740:750	33 children	740:750	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	8	37	gly	low-N-glycosylated	1083:1100	arg1	RESULTS					1015:1021	RESULTS	1015:1021	RESULTS In children up to 18 months of age, most TSH molecules	1015:1076	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	2	38	theme	normal	353:358	arg1	fetuses					376:382	normal third-trimester fetuses	353:382	normal third-trimester fetuses compared with normal adults	353:410	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	0	39	theme	Unique	0:5	arg1	Pattern					7:13	Unique Pattern	0:13	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children	0:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	1	40	theme	clinical	262:269	arg1	situations					271:280	different physiological and clinical situations	234:280	different physiological and clinical situations	234:280	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	11	41	theme	smaller	1514:1520	arg1	size					1522:1525	smaller size	1514:1525	smaller size	1514:1525	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	13	42	theme	molecules	1836:1844	arg1	explanation					1795:1805	a likely explanation	1786:1805	a likely explanation of the highly sulfonated TSH molecules in serum samples of infants	1786:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	42	theme	molecules	1836:1844	arg1	development					1702:1712	A delayed development	1692:1712	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth	1692:1781	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	9	43	theme	pituitary	1319:1327	arg1	extracts					1329:1336	pituitary extracts	1319:1336	pituitary extracts	1319:1336	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	8	44	theme	TSH	1064:1066	arg1	months					1044:1049	18 months	1041:1049	18 months of age	1041:1056	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	8	44	theme	TSH	1064:1066	arg1	molecules					1068:1076	most TSH molecules	1059:1076	most TSH molecules	1059:1076	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	11	45	from	molecules	1478:1486	arg1	children					1497:1504	young children	1491:1504	young children	1491:1504	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	13	46	theme	serum	1849:1853	arg1	samples					1855:1861	serum samples	1849:1861	serum samples of infants	1849:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	3	47	theme	young	477:481	arg1	children					483:490	young children	477:490	young children	477:490	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	6	48	theme	TSH	838:840	arg1	glycoforms					842:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms	793:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms	793:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	13	49	from	molecules	1836:1844	arg1	samples					1855:1861	serum samples	1849:1861	serum samples of infants	1849:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	2	50	from	fetuses	376:382	arg1	degree					287:292	The degree	283:292	The degree of sialylation of serum TSH	283:320	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	2	50	from	fetuses	376:382	arg1	low					346:348	low	346:348	low	346:348	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	11	51	theme	charged	1547:1553	arg1	size					1522:1525	smaller size	1514:1525	smaller size	1514:1525	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	2	52	theme	TSH	318:320	arg1	sialylation					297:307	sialylation	297:307	sialylation of serum TSH	297:320	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	1	53	theme	biological	202:211	arg1	properties					213:222	the biological properties	198:222	the biological properties of TSH	198:229	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	1	54	theme	CONTEXT	119:125	arg1	N-glycosylation					127:141	CONTEXT N-glycosylation	119:141	CONTEXT N-glycosylation	119:141	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	7	55	gly	N-glycosylation	940:954	arg1	extracts					987:994	pituitary extracts	977:994	pituitary extracts of 75 individuals	977:1012	The TSH N-glycosylation was also examined in pituitary extracts of 75 individuals.					
31169903	2	56	gly	sialylation	297:307	arg1	TSH					318:320	serum TSH	312:320	serum TSH	312:320	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	4	57	theme	young	624:628	arg1	children					630:637	young children	624:637	young children	624:637	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	10	58	contain	had	1380:1382	arg2	molecules					1403:1411	low-sialylated TSH molecules	1384:1411	low-sialylated TSH molecules	1384:1411	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	10	58	contain	had	1380:1382	arg1	Children					1351:1358	CONCLUSIONS Children	1339:1358	CONCLUSIONS Children up to age 18 months	1339:1378	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	8	59	dep	months	1044:1049	arg1	up					1035:1036	up	1035:1036	up	1035:1036	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	5	60	theme	OUTCOME	667:673	arg1	DESIGN					640:645	DESIGN	640:645	DESIGN	640:645	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	5	60	theme	OUTCOME	667:673	arg1	MEASURES					675:682	MAIN OUTCOME MEASURES	662:682	MAIN OUTCOME MEASURES	662:682	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	1	61	theme	physiological	244:256	arg1	situations					271:280	different physiological and clinical situations	234:280	different physiological and clinical situations	234:280	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	4	62	theme	circulating	595:605	arg1	molecules					611:619	circulating TSH molecules	595:619	circulating TSH molecules	595:619	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	13	63	theme	likely	1788:1793	arg1	explanation					1795:1805	a likely explanation	1786:1805	a likely explanation of the highly sulfonated TSH molecules in serum samples of infants	1786:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	63	theme	likely	1788:1793	arg1	development					1702:1712	A delayed development	1692:1712	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth	1692:1781	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	9	64	theme	N-glycosylation	1194:1208	arg1	similar					1214:1220	similar	1214:1220	similar	1214:1220	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	9	64	theme	N-glycosylation	1194:1208	arg1	degree					1184:1189	The degree	1180:1189	The degree of N-glycosylation	1180:1208	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	1	65	theme	glycan	147:152	arg1	composition					154:164	glycan composition	147:164	glycan composition	147:164	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	13	66	theme	receptor	1753:1760	arg1	function					1762:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	10	67	theme	TSH	1399:1401	arg1	molecules					1403:1411	low-sialylated TSH molecules	1384:1411	low-sialylated TSH molecules	1384:1411	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	2	68	from	low	346:348	arg1	fetuses					376:382	normal third-trimester fetuses	353:382	normal third-trimester fetuses compared with normal adults	353:410	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	1	69	theme	TSH	175:177	arg1	molecules					179:187	human TSH molecules	169:187	human TSH molecules	169:187	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
31169903	0	70	from	Molecules	71:79	arg1	Serum					84:88	Serum	84:88	Serum of Children	84:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	9	71	theme	pituitary	1235:1243	arg1	extracts					1245:1252	pituitary extracts	1235:1252	pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts	1235:1336	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	13	72	theme	liver	1721:1725	arg1	function					1762:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	the liver SO3-N-acetylgalactosamine receptor function	1717:1769	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	5	73	theme	Serum	684:688	arg1	samples					690:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples	640:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples	640:696	DESIGN, PARTICIPANTS, MAIN OUTCOME MEASURES Serum samples were obtained from euthyroid individuals: 33 children, age 2 weeks to 3 years, and 264 adults.					
31169903	4	74	gly	N-glycosylation	553:567	arg1	molecules					611:619	circulating TSH molecules	595:619	circulating TSH molecules	595:619	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	4	74	gly	N-glycosylation	553:567	arg1	children					630:637	young children	624:637	young children	624:637	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	9	75	from	serum	1225:1229	arg1	similar					1214:1220	similar	1214:1220	similar	1214:1220	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	9	75	from	serum	1225:1229	arg1	degree					1184:1189	The degree	1180:1189	The degree of N-glycosylation	1180:1208	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	11	76	theme	TSH	1474:1476	arg1	molecules					1478:1486	Most TSH molecules	1469:1486	Most TSH molecules in young children	1469:1504	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	9	77	from	similar	1214:1220	arg1	extracts					1245:1252	pituitary extracts	1235:1252	pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts	1235:1336	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	9	77	from	similar	1214:1220	arg1	serum					1225:1229	serum	1225:1229	serum	1225:1229	The degree of N-glycosylation was similar in serum and pituitary extracts up to 3 months of age and after that was higher in serum than in pituitary extracts.					
31169903	7	78	theme	individuals	1002:1012	arg1	extracts					987:994	pituitary extracts	977:994	pituitary extracts of 75 individuals	977:1012	The TSH N-glycosylation was also examined in pituitary extracts of 75 individuals.					
31169903	0	79	dep	Months	111:116	arg1	Pattern					7:13	Unique Pattern	0:13	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children	0:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	0	80	from	Pattern	7:13	arg1	Molecules					71:79	TSH Molecules	67:79	TSH Molecules in Serum of Children	67:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	7	81	theme	pituitary	977:985	arg1	extracts					987:994	pituitary extracts	977:994	pituitary extracts of 75 individuals	977:1012	The TSH N-glycosylation was also examined in pituitary extracts of 75 individuals.					
31169903	12	82	theme	TSH	1677:1679	arg1	receptors					1681:1689	extrathyroidal TSH receptors	1662:1689	extrathyroidal TSH receptors	1662:1689	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	6	83	gly	tri-glycosylated	821:836	arg1	TSH					838:840	tri-glycosylated TSH	821:840	tri-glycosylated TSH	821:840	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	0	84	theme	Children	93:100	arg1	Serum					84:88	Serum	84:88	Serum of Children	84:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	8	85	theme	age	1054:1056	arg1	months					1044:1049	18 months	1041:1049	18 months of age	1041:1056	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	8	85	theme	age	1054:1056	arg1	molecules					1068:1076	most TSH molecules	1059:1076	most TSH molecules	1059:1076	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	12	86	theme	high	1631:1634	arg1	biopotency					1636:1645	a high biopotency	1629:1645	a high biopotency at thyroid and extrathyroidal TSH receptors	1629:1689	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	10	87	theme	third-trimester	1444:1458	arg1	fetuses					1460:1466	third-trimester fetuses	1444:1466	third-trimester fetuses	1444:1466	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	4	88	theme	molecules	611:619	arg1	N-glycosylation					553:567	N-glycosylation	553:567	N-glycosylation	553:567	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	4	88	theme	molecules	611:619	arg1	composition					580:590	glycan composition	573:590	glycan composition	573:590	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	13	89	theme	infants	1866:1872	arg1	samples					1855:1861	serum samples	1849:1861	serum samples of infants	1849:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	2	90	theme	third-trimester	360:374	arg1	fetuses					376:382	normal third-trimester fetuses	353:382	normal third-trimester fetuses compared with normal adults	353:410	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	8	91	theme	most	1059:1062	arg1	months					1044:1049	18 months	1041:1049	18 months of age	1041:1056	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	8	91	theme	most	1059:1062	arg1	molecules					1068:1076	most TSH molecules	1059:1076	most TSH molecules	1059:1076	RESULTS In children up to 18 months of age, most TSH molecules were low-N-glycosylated, high-sulfonated, and low-sialylated compared with older children and adults.					
31169903	13	92	theme	TSH	1832:1834	arg1	molecules					1836:1844	the highly sulfonated TSH molecules	1810:1844	the highly sulfonated TSH molecules in serum samples of infants	1810:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	93	from	explanation	1795:1805	arg1	samples					1855:1861	serum samples	1849:1861	serum samples of infants	1849:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	0	94	theme	N-Glycosylation	18:32	arg1	Pattern					7:13	Unique Pattern	0:13	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children	0:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	7	95	theme	TSH	936:938	arg1	N-glycosylation					940:954	The TSH N-glycosylation	932:954	The TSH N-glycosylation	932:954	The TSH N-glycosylation was also examined in pituitary extracts of 75 individuals.					
31169903	12	96	theme	low	1606:1608	arg1	sialylation					1610:1620	The low sialylation	1602:1620	The low sialylation	1602:1620	The low sialylation favors a high biopotency at thyroid and extrathyroidal TSH receptors.					
31169903	10	97	gly	low-sialylated	1384:1397	arg1	molecules					1403:1411	low-sialylated TSH molecules	1384:1411	low-sialylated TSH molecules	1384:1411	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	4	98	theme	glycan	573:578	arg1	composition					580:590	glycan composition	573:590	glycan composition	573:590	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	6	99	gly	glycoforms	842:851	arg1	TSH					813:815	di-glycosylated TSH	797:815	di-glycosylated TSH	797:815	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	6	99	gly	glycoforms	842:851	arg1	TSH					838:840	tri-glycosylated TSH	821:840	tri-glycosylated TSH	821:840	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	2	100	theme	normal	398:403	arg1	adults					405:410	normal adults	398:410	normal adults	398:410	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	0	101	theme	Sialylation	35:45	arg1	Pattern					7:13	Unique Pattern	0:13	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children	0:100	Unique Pattern of N-Glycosylation, Sialylation, and Sulfonation on TSH Molecules in Serum of Children Up to 18 Months.					
31169903	3	102	from	glycoforms	433:442	arg1	children					483:490	young children	477:490	young children	477:490	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	4	103	from	composition	580:590	arg1	children					630:637	young children	624:637	young children	624:637	OBJECTIVE To characterize N-glycosylation and glycan composition of circulating TSH molecules in young children.					
31169903	3	104	gly	glycoforms	433:442	arg1	TSH					429:431	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	3	104	gly	glycoforms	433:442	arg2	children					483:490	young children	477:490	young children	477:490	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	6	105	theme	tri-glycosylated	821:836	arg1	TSH					838:840	tri-glycosylated TSH	821:840	tri-glycosylated TSH	821:840	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	11	106	theme	target	1586:1591	arg1	tissues					1593:1599	their target tissues	1580:1599	their target tissues	1580:1599	Most TSH molecules in young children were of smaller size and less negatively charged, favoring transport into their target tissues.					
31169903	2	107	theme	serum	312:316	arg1	TSH					318:320	serum TSH	312:320	serum TSH	312:320	The degree of sialylation of serum TSH was reported to be very low in normal third-trimester fetuses compared with normal adults.					
31169903	6	108	theme	TSH	813:815	arg1	glycoforms					842:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms	793:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms	793:851	The di-glycosylated TSH and tri-glycosylated TSH glycoforms were determined and characterized with respect to sialylation and sulfonation.					
31169903	13	109	from	samples	1855:1861	arg1	explanation					1795:1805	a likely explanation	1786:1805	a likely explanation of the highly sulfonated TSH molecules in serum samples of infants	1786:1872	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	13	109	from	samples	1855:1861	arg1	development					1702:1712	A delayed development	1692:1712	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth	1692:1781	A delayed development of the liver SO3-N-acetylgalactosamine receptor function after birth is a likely explanation of the highly sulfonated TSH molecules in serum samples of infants.					
31169903	3	110	theme	TSH	429:431	arg1	glycoforms					433:442	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms	413:442	The circulating TSH glycoforms and their glycan compositions in young children have hitherto not been determined.					
31169903	10	111	theme	CONCLUSIONS	1339:1349	arg1	Children					1351:1358	CONCLUSIONS Children	1339:1358	CONCLUSIONS Children up to age 18 months	1339:1378	CONCLUSIONS Children up to age 18 months had low-sialylated TSH molecules, similar to those reported for third-trimester fetuses.					
31169903	1	112	theme	TSH	227:229	arg1	properties					213:222	the biological properties	198:222	the biological properties of TSH	198:229	CONTEXT N-glycosylation and glycan composition of human TSH molecules modulate the biological properties of TSH in different physiological and clinical situations.					
30539395	7	0	theme	colony	1005:1010	arg1	density					1012:1018	colony density	1005:1018	colony density	1005:1018	In addition, colony density increased when TB-EPS was added, compared to that of MX-EPS.					
30539395	3	1	contain	containing	555:564	arg1	medium					548:553	a medium	546:553	a medium containing EPS, Ca2+ ion, and Mg2+ ion	546:592	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	3	1	contain	containing	555:564	arg2	ion					576:578	Ca2+ ion	571:578	Ca2+ ion	571:578	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	3	1	contain	containing	555:564	arg2	ion					590:592	Mg2+ ion	585:592	Mg2+ ion	585:592	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	3	1	contain	containing	555:564	arg2	EPS					566:568	EPS	566:568	EPS	566:568	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	3	2	theme	type	618:621	arg1	effect					603:608	the effect	599:608	the effect of each type of EPS on the colony formation of M. aeruginosa	599:669	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	8	3	from	present	1221:1227	arg1	TB-EPS					1232:1237	TB-EPS	1232:1237	TB-EPS	1232:1237	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	0	4	theme	colony	103:108	arg1	formation					110:118	the colony formation	99:118	the colony formation of unicellular Microcystis aeruginosa	99:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	1	5	theme	cyanobacterial	303:316	arg1	blooms					318:323	cyanobacterial blooms	303:323	cyanobacterial blooms	303:323	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	4	6	contain	contained	776:784	arg2	ions					800:803	Ca2+ and Mg2+ ions	786:803	Ca2+ and Mg2+ ions	786:803	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	4	6	contain	contained	776:784	arg1	medium					769:774	the TB-EPS medium	758:774	the TB-EPS medium	758:774	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	9	7	theme	Ca2+	1270:1273	arg1	ion					1275:1277	Ca2+ ion	1270:1277	Ca2+ ion chelated with EDTA	1270:1296	During the MX-EPS extraction, Ca2+ ion chelated with EDTA was removed during ethanol precipitation.					
30539395	8	8	attach	present	1221:1227	arg2	ion					1217:1219	Ca2+ ion	1212:1219	Ca2+ ion present in TB-EPS	1212:1237	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	8	8	attach	present	1221:1227	arg1	TB-EPS					1232:1237	TB-EPS	1232:1237	TB-EPS	1232:1237	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	4	9	theme	Ca2+	786:789	arg1	ions					800:803	Ca2+ and Mg2+ ions	786:803	Ca2+ and Mg2+ ions	786:803	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	6	10	located	present	952:958	arg1	concentration					972:984	250 mg/L concentration	963:984	250 mg/L concentration each	963:989	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	6	10	located	present	952:958	arg2	ions					942:945	Ca2+ and Mg2+ ions	928:945	Ca2+ and Mg2+ ions	928:945	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	6	11	theme	formation	878:886	arg1	experiment					888:897	the colony formation experiment	867:897	the colony formation experiment	867:897	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	8	12	theme	Ca2+	1212:1215	arg1	ion					1217:1219	Ca2+ ion	1212:1219	Ca2+ ion present in TB-EPS	1212:1237	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	6	13	theme	mg/L	967:970	arg1	concentration					972:984	250 mg/L concentration	963:984	250 mg/L concentration each	963:989	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	1	14	theme	different	331:339	arg1	methods					341:347	different methods	331:347	different methods	331:347	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	8	15	contain	containing	1123:1132	arg1	medium					1116:1121	the medium	1112:1121	the medium containing only TB-EPS (100 mg/L)	1112:1155	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	8	15	contain	containing	1123:1132	arg2	mg/L					1151:1154	100 mg/L	1147:1154	100 mg/L	1147:1154	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	8	15	contain	containing	1123:1132	arg2	TB-EPS					1139:1144	only TB-EPS	1134:1144	only TB-EPS (100 mg/L)	1134:1155	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	6	16	theme	250	963:965	arg1	mg/L					967:970	mg/L	967:970	mg/L	967:970	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	6	17	from	concentration	972:984	arg1	present					952:958	present	952:958	present	952:958	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	3	18	theme	colony	637:642	arg1	formation					644:652	the colony formation	633:652	the colony formation of M. aeruginosa	633:669	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	4	19	theme	TB-EPS	762:767	arg1	medium					769:774	the TB-EPS medium	758:774	the TB-EPS medium	758:774	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	0	20	theme	cyanobacterial	74:87	arg1	blooms					89:94	cyanobacterial blooms	74:94	cyanobacterial blooms	74:94	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	8	21	theme	present	1221:1227	arg1	ion					1217:1219	Ca2+ ion	1212:1219	Ca2+ ion present in TB-EPS	1212:1237	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	10	22	theme	Ca2+	1442:1445	arg1	ions					1447:1450	Ca2+ ions	1442:1450	Ca2+ ions	1442:1450	Therefore, the extraction protocol followed for TB-EPS was better than that of MX-EPS for maintaining Ca2+ ions, and thereby maintaining an EPS composition that enables for colony formation.					
30539395	5	23	located	detected	829:836	arg1	medium					852:857	the MX-EPS medium	841:857	the MX-EPS medium	841:857	These cations were not detected in the MX-EPS medium.					
30539395	5	23	located	detected	829:836	arg2	cations					812:818	These cations	806:818	These cations	806:818	These cations were not detected in the MX-EPS medium.					
30539395	6	24	theme	colony	871:876	arg1	formation					878:886	the colony formation	867:886	the colony formation experiment	867:897	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	1	25	theme	extracellular	187:199	arg1	EPS					269:271	tightly bound EPS	255:271	tightly bound EPS (TB-EPS)	255:280	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	25	theme	extracellular	187:199	arg1	EPS					218:220	EPS	218:220	EPS	218:220	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	25	theme	extracellular	187:199	arg1	EPS					238:240	mixed EPS	232:240	mixed EPS (MX-EPS)	232:249	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	25	theme	extracellular	187:199	arg1	polysaccharides					201:215	extracellular polysaccharides	187:215	extracellular polysaccharides (EPS)	187:221	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	0	26	theme	types	21:25	arg1	Effects					0:6	Effects	0:6	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.	0:157	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	4	27	theme	carboxy	721:727	arg1	groups					729:734	more carboxy groups	716:734	more carboxy groups	716:734	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	0	28	attach	isolated	60:67	arg1	blooms					89:94	cyanobacterial blooms	74:94	cyanobacterial blooms	74:94	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	0	28	attach	isolated	60:67	arg2	polysaccharides					44:58	extracellular polysaccharides	30:58	extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa	30:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	5	29	theme	MX-EPS	845:850	arg1	medium					852:857	the MX-EPS medium	841:857	the MX-EPS medium	841:857	These cations were not detected in the MX-EPS medium.					
30539395	2	30	theme	elemental	395:403	arg1	composition					405:415	elemental composition	395:415	elemental composition	395:415	To evaluate their compositional differences, elemental composition, FTIR, and TG/DTA profile were measured for both EPS samples.					
30539395	0	31	theme	different	11:19	arg1	types					21:25	different types	11:25	different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa	11:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	1	32	theme	bound	263:267	arg1	TB-EPS					274:279	TB-EPS	274:279	TB-EPS	274:279	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	32	theme	bound	263:267	arg1	EPS					269:271	tightly bound EPS	255:271	tightly bound EPS (TB-EPS)	255:280	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	32	theme	bound	263:267	arg1	polysaccharides					201:215	extracellular polysaccharides	187:215	extracellular polysaccharides (EPS)	187:221	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	8	33	theme	only	1134:1137	arg1	TB-EPS					1139:1144	only TB-EPS	1134:1144	only TB-EPS (100 mg/L)	1134:1155	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	8	33	theme	only	1134:1137	arg1	mg/L					1151:1154	100 mg/L	1147:1154	100 mg/L	1147:1154	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	0	34	theme	extracellular	30:42	arg1	polysaccharides					44:58	extracellular polysaccharides	30:58	extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa	30:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	3	35	theme	aeruginosa	660:669	arg1	formation					644:652	the colony formation	633:652	the colony formation of M. aeruginosa	633:669	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	9	36	theme	ethanol	1317:1323	arg1	precipitation					1325:1337	ethanol precipitation	1317:1337	ethanol precipitation	1317:1337	During the MX-EPS extraction, Ca2+ ion chelated with EDTA was removed during ethanol precipitation.					
30539395	9	37	theme	MX-EPS	1251:1256	arg1	extraction					1258:1267	the MX-EPS extraction	1247:1267	the MX-EPS extraction	1247:1267	During the MX-EPS extraction, Ca2+ ion chelated with EDTA was removed during ethanol precipitation.					
30539395	10	38	theme	EPS	1480:1482	arg1	composition					1484:1494	an EPS composition	1477:1494	an EPS composition that enables for colony formation	1477:1528	Therefore, the extraction protocol followed for TB-EPS was better than that of MX-EPS for maintaining Ca2+ ions, and thereby maintaining an EPS composition that enables for colony formation.					
30539395	6	39	theme	Mg2+	937:940	arg1	ions					942:945	Ca2+ and Mg2+ ions	928:945	Ca2+ and Mg2+ ions	928:945	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	0	40	theme	aeruginosa	147:156	arg1	formation					110:118	the colony formation	99:118	the colony formation of unicellular Microcystis aeruginosa	99:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	3	41	theme	Mg2+	585:588	arg1	ion					590:592	Mg2+ ion	585:592	Mg2+ ion	585:592	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	6	42	dep	concentration	972:984	arg1	each					986:989	each	986:989	each	986:989	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	2	43	theme	compositional	368:380	arg1	differences					382:392	their compositional differences	362:392	their compositional differences	362:392	To evaluate their compositional differences, elemental composition, FTIR, and TG/DTA profile were measured for both EPS samples.					
30539395	2	44	theme	TG/DTA	428:433	arg1	profile					435:441	TG/DTA profile	428:441	TG/DTA profile	428:441	To evaluate their compositional differences, elemental composition, FTIR, and TG/DTA profile were measured for both EPS samples.					
30539395	6	45	attach	present	952:958	arg1	concentration					972:984	250 mg/L concentration	963:984	250 mg/L concentration each	963:989	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	6	45	attach	present	952:958	arg2	ions					942:945	Ca2+ and Mg2+ ions	928:945	Ca2+ and Mg2+ ions	928:945	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	0	46	theme	polysaccharides	44:58	arg1	types					21:25	different types	11:25	different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa	11:156	Effects of different types of extracellular polysaccharides isolated from cyanobacterial blooms on the colony formation of unicellular Microcystis aeruginosa.					
30539395	4	47	theme	Mg2+	795:798	arg1	ions					800:803	Ca2+ and Mg2+ ions	786:803	Ca2+ and Mg2+ ions	786:803	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	10	48	theme	colony	1513:1518	arg1	formation					1520:1528	colony formation	1513:1528	colony formation	1513:1528	Therefore, the extraction protocol followed for TB-EPS was better than that of MX-EPS for maintaining Ca2+ ions, and thereby maintaining an EPS composition that enables for colony formation.					
30539395	8	49	located	observed	1100:1107	arg1	medium					1116:1121	the medium	1112:1121	the medium containing only TB-EPS (100 mg/L)	1112:1155	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	8	49	located	observed	1100:1107	arg2	Colonies					1081:1088	Colonies	1081:1088	Colonies	1081:1088	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	4	50	theme	more	716:719	arg1	groups					729:734	more carboxy groups	716:734	more carboxy groups	716:734	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	6	51	theme	Ca2+	928:931	arg1	ions					942:945	Ca2+ and Mg2+ ions	928:945	Ca2+ and Mg2+ ions	928:945	During the colony formation experiment, colonies were observed when Ca2+ and Mg2+ ions were present at 250 mg/L concentration each.					
30539395	10	52	theme	extraction	1355:1364	arg1	better					1399:1404	better	1399:1404	better	1399:1404	Therefore, the extraction protocol followed for TB-EPS was better than that of MX-EPS for maintaining Ca2+ ions, and thereby maintaining an EPS composition that enables for colony formation.					
30539395	10	52	theme	extraction	1355:1364	arg1	protocol					1366:1373	the extraction protocol	1351:1373	the extraction protocol followed for TB-EPS	1351:1393	Therefore, the extraction protocol followed for TB-EPS was better than that of MX-EPS for maintaining Ca2+ ions, and thereby maintaining an EPS composition that enables for colony formation.					
30539395	2	53	theme	EPS	466:468	arg1	samples					470:476	both EPS samples	461:476	both EPS samples	461:476	To evaluate their compositional differences, elemental composition, FTIR, and TG/DTA profile were measured for both EPS samples.					
30539395	3	54	theme	Ca2+	571:574	arg1	ion					576:578	Ca2+ ion	571:578	Ca2+ ion	571:578	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	1	55	theme	polysaccharides	201:215	arg1	types					178:182	two types	174:182	two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS),	174:281	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	3	56	from	effect	603:608	arg1	formation					644:652	the colony formation	633:652	the colony formation of M. aeruginosa	633:669	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	3	57	theme	EPS	626:628	arg1	type					618:621	each type	613:621	each type of EPS	613:628	Following that, unicellular Microcystis aeruginosa was cultured in a medium containing EPS, Ca2+ ion, and Mg2+ ion, and the effect of each type of EPS on the colony formation of M. aeruginosa was examined.					
30539395	4	58	contain	had	712:714	arg1	TB-EPS					705:710	TB-EPS	705:710	TB-EPS	705:710	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	4	58	contain	had	712:714	arg2	groups					729:734	more carboxy groups	716:734	more carboxy groups	716:734	Results showed that TB-EPS had more carboxy groups than MX-EPS, and that the TB-EPS medium contained Ca2+ and Mg2+ ions.					
30539395	8	59	from	TB-EPS	1232:1237	arg1	present					1221:1227	present	1221:1227	present	1221:1227	Colonies were also observed in the medium containing only TB-EPS (100 mg/L), indicating that M. aeruginosa can form colonies using Ca2+ ion present in TB-EPS.					
30539395	1	60	theme	mixed	232:236	arg1	MX-EPS					243:248	MX-EPS	243:248	MX-EPS	243:248	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	60	theme	mixed	232:236	arg1	polysaccharides					201:215	extracellular polysaccharides	187:215	extracellular polysaccharides (EPS)	187:221	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30539395	1	60	theme	mixed	232:236	arg1	EPS					238:240	mixed EPS	232:240	mixed EPS (MX-EPS)	232:249	In this study, two types of extracellular polysaccharides (EPS), namely, mixed EPS (MX-EPS) and tightly bound EPS (TB-EPS), were extracted from cyanobacterial blooms using different methods.					
30336974	6	0	theme	cell	1093:1096	arg1	glycoproteins					1106:1118	cell surface glycoproteins	1093:1118	cell surface glycoproteins in both the cell lines with varying degrees of efficiency	1093:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	12	1	dep	expression	1828:1837	arg1	the					1824:1826	the	1824:1826	the	1824:1826	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	1	2	theme	translational	256:268	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	6	3	theme	efficiency	1167:1176	arg1	degrees					1156:1162	varying degrees	1148:1162	varying degrees of efficiency	1148:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	5	4	theme	metabolic	831:839	arg1	strategy					866:873	the metabolic glycan engineering (MGE) strategy	827:873	the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue,	827:967	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	1	5	theme	glycosylation	200:212	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	5	theme	glycosylation	200:212	arg1	structure					166:174	structure	166:174	structure	166:174	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	5	theme	glycosylation	200:212	arg1	roles					191:195	functional roles	180:195	functional roles of glycosylation	180:212	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	9	6	from	antigen	1505:1511	arg1	lines					1527:1531	these cell lines	1516:1531	these cell lines	1516:1531	Additionally, we studied how the glycan modifications vary on a given CD antigen in these cell lines.					
30336974	0	7	theme	myeloid	129:135	arg1	cells					137:141	myeloid cells	129:141	myeloid cells	129:141	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	10	8	theme	glycan-dependent	1622:1637	arg1	antibodies					1662:1671	glycan-dependent and glycan-independent antibodies	1622:1671	glycan-dependent and glycan-independent antibodies	1622:1671	Particularly, MTOG on CD43 was differentially inhibited in K562 and U937 as revealed by glycan-dependent and glycan-independent antibodies.					
30336974	9	9	theme	given	1496:1500	arg1	antigen					1505:1511	a given CD antigen	1494:1511	a given CD antigen in these cell lines	1494:1531	Additionally, we studied how the glycan modifications vary on a given CD antigen in these cell lines.					
30336974	8	10	theme	glycosylation	1300:1312	arg1	machinery					1314:1322	endogenous glycosylation machinery	1289:1322	endogenous glycosylation machinery of K562	1289:1330	It was observed that endogenous glycosylation machinery of K562 is relatively more stringent for selecting GalNTGc whereas U937 is flexible towards this selection.					
30336974	3	11	gly	O-glycosylation	552:566	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	11	gly	O-glycosylation	552:566	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	11	gly	O-glycosylation	552:566	arg1	lines					616:620	two functionally divergent human cell lines	578:620	two functionally divergent human cell lines	578:620	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	12	theme	lines	616:620	arg1	O-glycosylation					552:566	the mucin-type O-glycosylation	537:566	the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins	537:709	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	12	theme	lines	616:620	arg1	MTOG					569:572	MTOG	569:572	MTOG	569:572	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	11	13	theme	global	1736:1741	arg1	effects					1743:1749	global effects	1736:1749	global effects on both cell lines	1736:1768	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	7	14	theme	differential	1235:1246	arg1	inhibition					1248:1257	differential inhibition	1235:1257	differential inhibition of MTOG	1235:1265	Notably, metabolic incorporation of GalNTGc resulted in differential inhibition of MTOG.					
30336974	1	15	theme	challenging	332:342	arg1	Investigations					144:157	Investigations	144:157	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes	144:319	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	15	theme	challenging	332:342	arg1	task					344:347	a challenging task	330:347	a challenging task	330:347	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	3	16	theme	human	605:609	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	16	theme	human	605:609	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	16	theme	human	605:609	arg1	lines					616:620	two functionally divergent human cell lines	578:620	two functionally divergent human cell lines	578:620	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	5	17	theme	MGE	861:863	arg1	strategy					866:873	the metabolic glycan engineering (MGE) strategy	827:873	the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue,	827:967	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	0	18	theme	peracetyl	72:80	arg1	Ac5GalNTGc					114:123	Ac5GalNTGc	114:123	Ac5GalNTGc	114:123	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	0	18	theme	peracetyl	72:80	arg1	N-thioglycolyl-d-galactosamine					82:111	peracetyl N-thioglycolyl-d-galactosamine	72:111	peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells	72:141	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	12	19	theme	cell	1925:1928	arg1	surface					1930:1936	cell surface	1925:1936	cell surface	1925:1936	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	1	20	from	structure	166:174	arg1	proteins					286:293	proteins	286:293	proteins	286:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	20	from	structure	166:174	arg1	processes					311:319	biological processes	300:319	biological processes	300:319	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	12	21	from	glycosylation	1908:1920	arg1	surface					1930:1936	cell surface	1925:1936	cell surface	1925:1936	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	12	21	from	glycosylation	1908:1920	arg1	glycoproteins					1961:1973	specific glycoproteins	1952:1973	specific glycoproteins	1952:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	11	22	from	effect	1699:1704	arg1	CD43					1716:1719	CD43	1716:1719	CD43	1716:1719	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	11	23	theme	cell	1759:1762	arg1	lines					1764:1768	both cell lines	1754:1768	both cell lines	1754:1768	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	5	24	theme	synthetic	942:950	arg1	N-thioglycolyl-d-galactosamine					895:924	the peracetyl N-thioglycolyl-d-galactosamine	881:924	the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc)	881:937	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	5	24	theme	synthetic	942:950	arg1	analogue					959:966	a synthetic GalNAc analogue	940:966	a synthetic GalNAc analogue	940:966	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	7	25	theme	metabolic	1188:1196	arg1	incorporation					1198:1210	metabolic incorporation	1188:1210	metabolic incorporation of GalNTGc	1188:1221	Notably, metabolic incorporation of GalNTGc resulted in differential inhibition of MTOG.					
30336974	1	26	dep	structure	166:174	arg1	the					162:164	the	162:164	the	162:164	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	2	27	theme	specific	393:400	arg1	type					407:410	the specific cell type	389:410	the specific cell type	389:410	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	0	28	theme	Differential	0:11	arg1	inhibition					13:22	Differential inhibition	0:22	Differential inhibition of mucin-type O-glycosylation (MTOG)	0:59	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	12	29	theme	various	1855:1861	arg1	transferases					1872:1883	various glycosyl transferases	1855:1883	various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins	1855:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	10	30	from	MTOG	1548:1551	arg1	CD43					1556:1559	CD43	1556:1559	CD43	1556:1559	Particularly, MTOG on CD43 was differentially inhibited in K562 and U937 as revealed by glycan-dependent and glycan-independent antibodies.					
30336974	3	31	theme	chronic	629:635	arg1	leukemia					645:652	chronic myeloid leukemia	629:652	chronic myeloid leukemia	629:652	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	31	theme	chronic	629:635	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	0	32	theme	O-glycosylation	38:52	arg1	inhibition					13:22	Differential inhibition	0:22	Differential inhibition of mucin-type O-glycosylation (MTOG)	0:59	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	12	33	theme	transferases	1872:1883	arg1	activity					1843:1850	activity	1843:1850	activity	1843:1850	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	12	33	theme	transferases	1872:1883	arg1	expression					1828:1837	expression	1828:1837	expression	1828:1837	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	2	34	theme	Glycan	350:355	arg1	modifications					357:369	Glycan modifications	350:369	Glycan modifications	350:369	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	4	35	theme	N-acetyl-α-d-galactosamine	749:774	arg1	addition					737:744	the addition	733:744	the addition of N-acetyl-α-d-galactosamine (GalNAc) to Ser/Thr of glycoproteins	733:811	MTOG is initiated by the addition of N-acetyl-α-d-galactosamine (GalNAc) to Ser/Thr of glycoproteins.					
30336974	1	36	theme	complex	230:236	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	12	37	theme	MGE	1787:1789	arg1	Consequences					1771:1782	Consequences	1771:1782	Consequences of MGE using GalNAc analogues	1771:1812	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	12	38	theme	GalNAc	1797:1802	arg1	analogues					1804:1812	GalNAc analogues	1797:1812	GalNAc analogues	1797:1812	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	6	39	theme	surface	1098:1104	arg1	glycoproteins					1106:1118	cell surface glycoproteins	1093:1118	cell surface glycoproteins in both the cell lines with varying degrees of efficiency	1093:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	1	40	theme	post	251:254	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	3	41	from	aim	495:497	arg1	study					485:489	the present study	473:489	the present study	473:489	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	42	theme	myeloid	695:701	arg1	origins					703:709	myeloid origins	695:709	myeloid origins	695:709	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	9	43	theme	glycan	1465:1470	arg1	modifications					1472:1484	the glycan modifications	1461:1484	the glycan modifications	1461:1484	Additionally, we studied how the glycan modifications vary on a given CD antigen in these cell lines.					
30336974	1	44	theme	functional	180:189	arg1	roles					191:195	functional roles	180:195	functional roles of glycosylation	180:212	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	6	45	with	glycoproteins	1106:1118	arg1	degrees					1156:1162	varying degrees	1148:1162	varying degrees of efficiency	1148:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	4	46	theme	glycoproteins	799:811	arg1	Ser/Thr					788:794	Ser/Thr	788:794	Ser/Thr of glycoproteins	788:811	MTOG is initiated by the addition of N-acetyl-α-d-galactosamine (GalNAc) to Ser/Thr of glycoproteins.					
30336974	5	47	theme	glycan	841:846	arg1	strategy					866:873	the metabolic glycan engineering (MGE) strategy	827:873	the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue,	827:967	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	6	48	theme	varying	1148:1154	arg1	degrees					1156:1162	varying degrees	1148:1162	varying degrees of efficiency	1148:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	1	49	from	modification	270:281	arg1	proteins					286:293	proteins	286:293	proteins	286:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	49	from	modification	270:281	arg1	processes					311:319	biological processes	300:319	biological processes	300:319	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	12	50	gly	glycoproteins	1961:1973	arg1	glycoproteins					1961:1973	specific glycoproteins	1952:1973	specific glycoproteins	1952:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	3	51	theme	mucin-type	541:550	arg1	O-glycosylation					552:566	the mucin-type O-glycosylation	537:566	the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins	537:709	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	51	theme	mucin-type	541:550	arg1	MTOG					569:572	MTOG	569:572	MTOG	569:572	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	4	52	gly	glycoproteins	799:811	arg1	glycoproteins					799:811	glycoproteins	799:811	glycoproteins	799:811	MTOG is initiated by the addition of N-acetyl-α-d-galactosamine (GalNAc) to Ser/Thr of glycoproteins.					
30336974	0	53	from	N-thioglycolyl-d-galactosamine	82:111	arg1	cells					137:141	myeloid cells	129:141	myeloid cells	129:141	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	5	54	theme	engineering	848:858	arg1	strategy					866:873	the metabolic glycan engineering (MGE) strategy	827:873	the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue,	827:967	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	1	55	theme	intricate	219:227	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	9	56	theme	CD	1502:1503	arg1	antigen					1505:1511	a given CD antigen	1494:1511	a given CD antigen in these cell lines	1494:1531	Additionally, we studied how the glycan modifications vary on a given CD antigen in these cell lines.					
30336974	12	57	theme	specific	1952:1959	arg1	glycoproteins					1961:1973	specific glycoproteins	1952:1973	specific glycoproteins	1952:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	11	58	from	effects	1743:1749	arg1	lines					1764:1768	both cell lines	1754:1768	both cell lines	1754:1768	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	8	59	theme	endogenous	1289:1298	arg1	machinery					1314:1322	endogenous glycosylation machinery	1289:1322	endogenous glycosylation machinery of K562	1289:1330	It was observed that endogenous glycosylation machinery of K562 is relatively more stringent for selecting GalNTGc whereas U937 is flexible towards this selection.					
30336974	3	60	theme	cell	611:614	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	60	theme	cell	611:614	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	60	theme	cell	611:614	arg1	lines					616:620	two functionally divergent human cell lines	578:620	two functionally divergent human cell lines	578:620	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	5	61	theme	peracetyl	885:893	arg1	analogue					959:966	a synthetic GalNAc analogue	940:966	a synthetic GalNAc analogue	940:966	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	5	61	theme	peracetyl	885:893	arg1	Ac5GalNTGc					927:936	Ac5GalNTGc	927:936	Ac5GalNTGc	927:936	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	5	61	theme	peracetyl	885:893	arg1	N-thioglycolyl-d-galactosamine					895:924	the peracetyl N-thioglycolyl-d-galactosamine	881:924	the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc)	881:937	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	3	62	contain	having	688:693	arg2	origins					703:709	myeloid origins	695:709	myeloid origins	695:709	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	62	contain	having	688:693	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	62	contain	having	688:693	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	62	contain	having	688:693	arg1	lines					616:620	two functionally divergent human cell lines	578:620	two functionally divergent human cell lines	578:620	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	63	theme	divergent	595:603	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	63	theme	divergent	595:603	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	63	theme	divergent	595:603	arg1	lines					616:620	two functionally divergent human cell lines	578:620	two functionally divergent human cell lines	578:620	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	9	64	theme	cell	1522:1525	arg1	lines					1527:1531	these cell lines	1516:1531	these cell lines	1516:1531	Additionally, we studied how the glycan modifications vary on a given CD antigen in these cell lines.					
30336974	8	65	theme	K562	1327:1330	arg1	machinery					1314:1322	endogenous glycosylation machinery	1289:1322	endogenous glycosylation machinery of K562	1289:1330	It was observed that endogenous glycosylation machinery of K562 is relatively more stringent for selecting GalNTGc whereas U937 is flexible towards this selection.					
30336974	7	66	theme	GalNTGc	1215:1221	arg1	incorporation					1198:1210	metabolic incorporation	1188:1210	metabolic incorporation of GalNTGc	1188:1221	Notably, metabolic incorporation of GalNTGc resulted in differential inhibition of MTOG.					
30336974	1	67	from	roles	191:195	arg1	proteins					286:293	proteins	286:293	proteins	286:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	67	from	roles	191:195	arg1	processes					311:319	biological processes	300:319	biological processes	300:319	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	6	68	gly	glycoproteins	1106:1118	arg1	glycoproteins					1106:1118	cell surface glycoproteins	1093:1118	cell surface glycoproteins in both the cell lines with varying degrees of efficiency	1093:1176	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	12	69	gly	glycosylation	1908:1920	arg1	surface					1930:1936	cell surface	1925:1936	cell surface	1925:1936	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	12	69	gly	glycosylation	1908:1920	arg1	glycoproteins					1961:1973	specific glycoproteins	1952:1973	specific glycoproteins	1952:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	12	70	theme	global	1901:1906	arg1	glycosylation					1908:1920	global glycosylation	1901:1920	global glycosylation on cell surface as well as on specific glycoproteins	1901:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	5	71	theme	GalNAc	952:957	arg1	N-thioglycolyl-d-galactosamine					895:924	the peracetyl N-thioglycolyl-d-galactosamine	881:924	the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc)	881:937	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	5	71	theme	GalNAc	952:957	arg1	analogue					959:966	a synthetic GalNAc analogue	940:966	a synthetic GalNAc analogue	940:966	We exploited the metabolic glycan engineering (MGE) strategy using the peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc), a synthetic GalNAc analogue, to engineer the glycoconjugates.					
30336974	2	72	theme	cell	402:405	arg1	type					407:410	the specific cell type	389:410	the specific cell type	389:410	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	1	73	from	Investigations	144:157	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	73	from	Investigations	144:157	arg1	structure					166:174	structure	166:174	structure	166:174	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	1	73	from	Investigations	144:157	arg1	roles					191:195	functional roles	180:195	functional roles of glycosylation	180:212	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	2	74	theme	resting	442:448	arg1	state					463:467	resting or activated state	442:467	resting or activated state	442:467	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	10	75	theme	glycan-independent	1643:1660	arg1	antibodies					1662:1671	glycan-dependent and glycan-independent antibodies	1622:1671	glycan-dependent and glycan-independent antibodies	1622:1671	Particularly, MTOG on CD43 was differentially inhibited in K562 and U937 as revealed by glycan-dependent and glycan-independent antibodies.					
30336974	0	76	theme	mucin-type	27:36	arg1	MTOG					55:58	MTOG	55:58	MTOG	55:58	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	0	76	theme	mucin-type	27:36	arg1	O-glycosylation					38:52	mucin-type O-glycosylation	27:52	mucin-type O-glycosylation (MTOG)	27:59	Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells.					
30336974	12	77	theme	glycosyl	1863:1870	arg1	transferases					1872:1883	various glycosyl transferases	1855:1883	various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins	1855:1973	Consequences of MGE using GalNAc analogues depend on the expression and activity of various glycosyl transferases which determine global glycosylation on cell surface as well as on specific glycoproteins.					
30336974	11	78	theme	MGE	1709:1711	arg1	effect					1699:1704	the effect	1695:1704	the effect of MGE on CD43	1695:1719	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	11	78	theme	MGE	1709:1711	arg1	similar					1725:1731	similar	1725:1731	similar	1725:1731	It was observed that the effect of MGE on CD43 was similar to global effects on both cell lines.					
30336974	2	79	theme	developmental	417:429	arg1	stage					431:435	its developmental stage	413:435	its developmental stage	413:435	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	1	80	theme	biological	300:309	arg1	processes					311:319	biological processes	300:319	biological processes	300:319	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30336974	6	81	theme	cell	1132:1135	arg1	lines					1137:1141	both the cell lines	1123:1141	both the cell lines	1123:1141	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	2	82	theme	activated	453:461	arg1	state					463:467	resting or activated state	442:467	resting or activated state	442:467	Glycan modifications vary depending on the specific cell type, its developmental stage, and resting or activated state.					
30336974	3	83	theme	myeloid	637:643	arg1	leukemia					645:652	chronic myeloid leukemia	629:652	chronic myeloid leukemia	629:652	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	83	theme	myeloid	637:643	arg1	K562					623:626	K562	623:626	K562 (chronic myeloid leukemia)	623:653	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	84	theme	present	477:483	arg1	study					485:489	the present study	473:489	the present study	473:489	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	7	85	theme	MTOG	1262:1265	arg1	inhibition					1248:1257	differential inhibition	1235:1257	differential inhibition of MTOG	1235:1265	Notably, metabolic incorporation of GalNTGc resulted in differential inhibition of MTOG.					
30336974	3	86	theme	histiocytic	665:675	arg1	U937					659:662	U937	659:662	U937 (histiocytic lymphoma)	659:685	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	3	86	theme	histiocytic	665:675	arg1	lymphoma					677:684	histiocytic lymphoma	665:684	histiocytic lymphoma	665:684	In the present study, we aim to understand the differences between the mucin-type O-glycosylation (MTOG) of two functionally divergent human cell lines, K562 (chronic myeloid leukemia) and U937 (histiocytic lymphoma), having myeloid origins.					
30336974	6	87	from	glycoproteins	1106:1118	arg1	lines					1137:1141	both the cell lines	1123:1141	both the cell lines	1123:1141	Ac5GalNTGc was metabolized and incorporated as N-thioglycolyl-d-galactosamine (GalNTGc) in cell surface glycoproteins in both the cell lines with varying degrees of efficiency.					
30336974	1	88	theme	dynamic	243:249	arg1	modification					270:281	an intricate, complex, and dynamic post translational modification	216:281	an intricate, complex, and dynamic post translational modification on proteins	216:293	Investigations on the structure and functional roles of glycosylation - an intricate, complex, and dynamic post translational modification on proteins - in biological processes has been a challenging task.					
30708332	5	0	theme	average	673:679	arg1	energy					686:691	the average bond energy	669:691	the average bond energy of chemical compositions	669:716	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	3	1	contain	had	497:499	arg1	lignin					490:495	lignin	490:495	lignin	490:495	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	3	1	contain	had	497:499	arg2	rate					522:525	the highest residual rate	501:525	the highest residual rate	501:525	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	0	2	theme	protein	54:60	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	6	3	theme	reactions	886:894	arg1	number					868:873	a large number	860:873	a large number of surface reactions	860:894	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	4	4	theme	Kissinger-Akahira-Sunose	602:625	arg1	method					627:632	Kissinger-Akahira-Sunose method	602:632	Kissinger-Akahira-Sunose method	602:632	The apparent activation energy of these model compounds was calculated by Kissinger-Akahira-Sunose method.					
30708332	1	5	theme	corn	187:190	arg1	starch					192:197	corn starch	187:197	corn starch	187:197	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	6	6	theme	surface	878:884	arg1	reactions					886:894	surface reactions	878:894	surface reactions	878:894	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	1	7	theme	starch	192:197	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	0	8	theme	lignin	63:68	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	6	9	dep	controlled	987:996	arg1	surface					979:985	surface	979:985	surface	979:985	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	3	10	theme	widest	452:457	arg1	zone					481:484	the widest pyrolysis temperature zone	448:484	the widest pyrolysis temperature zone	448:484	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	2	11	dep	behavior	257:264	arg1	The					243:245	The	243:245	The	243:245	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	6	12	theme	model	954:958	arg1	compounds					960:968	other model compounds	948:968	other model compounds	948:968	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	5	13	theme	kinetic	639:645	arg1	results					647:653	The kinetic results	635:653	The kinetic results	635:653	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	7	14	theme	model	1056:1060	arg1	compounds					1062:1070	six model compounds	1052:1070	six model compounds	1052:1070	The thermodynamic parameters including G, ΔS, ΔH for six model compounds were also calculated.					
30708332	4	15	theme	model	568:572	arg1	compounds					574:582	these model compounds	562:582	these model compounds	562:582	The apparent activation energy of these model compounds was calculated by Kissinger-Akahira-Sunose method.					
30708332	0	16	theme	xylan	71:75	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	6	17	theme	large	862:866	arg1	number					868:873	a large number	860:873	a large number of surface reactions	860:894	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	5	18	theme	compositions	705:716	arg1	energy					686:691	the average bond energy	669:691	the average bond energy of chemical compositions	669:716	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	5	19	theme	chemical	696:703	arg1	compositions					705:716	chemical compositions	696:716	chemical compositions	696:716	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	5	20	theme	bond	681:684	arg1	energy					686:691	the average bond energy	669:691	the average bond energy of chemical compositions	669:716	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	1	21	theme	work	131:134	arg1	aim					119:121	The aim	115:121	The aim of this work	115:134	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	0	22	theme	Thermal	0:6	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	1	23	theme	soy	200:202	arg1	protein					204:210	soy protein	200:210	soy protein	200:210	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	4	24	theme	compounds	574:582	arg1	energy					552:557	The apparent activation energy	528:557	The apparent activation energy of these model compounds	528:582	The apparent activation energy of these model compounds was calculated by Kissinger-Akahira-Sunose method.					
30708332	0	25	theme	castor	25:30	arg1	oil					32:34	castor oil	25:34	castor oil	25:34	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	1	26	theme	protein	204:210	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	0	27	theme	cellulose	82:90	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	3	28	theme	pyrolysis	459:467	arg1	zone					481:484	the widest pyrolysis temperature zone	448:484	the widest pyrolysis temperature zone	448:484	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	2	29	theme	model	331:335	arg1	compounds					337:345	these model compounds	325:345	these model compounds	325:345	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	2	30	theme	compounds	337:345	arg1	kinetics					294:301	kinetics	294:301	kinetics	294:301	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	2	30	theme	compounds	337:345	arg1	evolution					283:291	gaseous product evolution	267:291	gaseous product evolution	267:291	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	2	30	theme	compounds	337:345	arg1	thermodynamics					307:320	thermodynamics	307:320	thermodynamics	307:320	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	2	30	theme	compounds	337:345	arg1	behavior					257:264	pyrolysis behavior	247:264	pyrolysis behavior	247:264	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	3	31	theme	temperature	469:479	arg1	zone					481:484	the widest pyrolysis temperature zone	448:484	the widest pyrolysis temperature zone	448:484	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	4	32	theme	apparent	532:539	arg1	energy					552:557	The apparent activation energy	528:557	The apparent activation energy of these model compounds	528:582	The apparent activation energy of these model compounds was calculated by Kissinger-Akahira-Sunose method.					
30708332	2	33	theme	heating	388:394	arg1	rates					396:400	high heating rates	383:400	high heating rates	383:400	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	1	34	theme	lignin	213:218	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	0	35	theme	fast	99:102	arg1	pyrolysis					104:112	fast pyrolysis	99:112	fast pyrolysis	99:112	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	0	36	theme	oil	32:34	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	3	37	contain	had	444:446	arg1	oil					440:442	castor oil	433:442	castor oil	433:442	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	3	37	contain	had	444:446	arg2	zone					481:484	the widest pyrolysis temperature zone	448:484	the widest pyrolysis temperature zone	448:484	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	2	38	theme	high	383:386	arg1	rates					396:400	high heating rates	383:400	high heating rates	383:400	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	3	39	theme	highest	505:511	arg1	rate					522:525	the highest residual rate	501:525	the highest residual rate	501:525	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	1	40	theme	pyrolysis	153:161	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	0	41	theme	starch	42:47	arg1	decomposition					8:20	Thermal decomposition	0:20	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.	0:113	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	1	42	theme	xylan	221:225	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	2	43	theme	pyrolysis	247:255	arg1	behavior					257:264	pyrolysis behavior	247:264	pyrolysis behavior	247:264	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	0	44	theme	corn	37:40	arg1	starch					42:47	corn starch	37:47	corn starch	37:47	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	6	45	theme	soy	900:902	arg1	protein					904:910	soy protein	900:910	soy protein	900:910	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	5	46	theme	lipid > lignin > starch > cellulose > protein > hemicellulose	738:798	arg1	order					729:733	the order	725:733	the order of lipid > lignin > starch > cellulose > protein > hemicellulose	725:798	The kinetic results revealed that the average bond energy of chemical compositions was in the order of lipid > lignin > starch > cellulose > protein > hemicellulose.					
30708332	3	47	theme	TG/DTG	407:412	arg1	curves					414:419	The TG/DTG curves	403:419	The TG/DTG curves	403:419	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	7	48	theme	thermodynamic	1003:1015	arg1	G					1038:1038	G	1038:1038	G	1038:1038	The thermodynamic parameters including G, ΔS, ΔH for six model compounds were also calculated.					
30708332	7	48	theme	thermodynamic	1003:1015	arg1	parameters					1017:1026	The thermodynamic parameters	999:1026	The thermodynamic parameters including G, ΔS, ΔH for six model compounds	999:1070	The thermodynamic parameters including G, ΔS, ΔH for six model compounds were also calculated.					
30708332	6	49	theme	factor	821:826	arg1	analysis					828:835	The pre-exponential factor analysis	801:835	The pre-exponential factor analysis	801:835	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	6	50	theme	pre-exponential	805:819	arg1	analysis					828:835	The pre-exponential factor analysis	801:835	The pre-exponential factor analysis	801:835	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	3	51	theme	castor	433:438	arg1	oil					440:442	castor oil	433:442	castor oil	433:442	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	0	52	theme	soy	50:52	arg1	protein					54:60	soy protein	50:60	soy protein	50:60	Thermal decomposition of castor oil, corn starch, soy protein, lignin, xylan, and cellulose during fast pyrolysis.					
30708332	3	53	theme	residual	513:520	arg1	rate					522:525	the highest residual rate	501:525	the highest residual rate	501:525	The TG/DTG curves showed that castor oil had the widest pyrolysis temperature zone and lignin had the highest residual rate.					
30708332	4	54	theme	activation	541:550	arg1	energy					552:557	The apparent activation energy	528:557	The apparent activation energy of these model compounds	528:582	The apparent activation energy of these model compounds was calculated by Kissinger-Akahira-Sunose method.					
30708332	2	55	theme	product	275:281	arg1	evolution					283:291	gaseous product evolution	267:291	gaseous product evolution	267:291	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	1	56	theme	castor	175:180	arg1	oil					182:184	castor oil	175:184	castor oil	175:184	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	1	57	theme	cellulose	232:240	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
30708332	6	58	theme	other	948:952	arg1	compounds					960:968	other model compounds	948:968	other model compounds	948:968	The pre-exponential factor analysis showed that there were a large number of surface reactions for soy protein and xylan during pyrolysis, however other model compounds were not surface controlled.					
30708332	2	59	theme	gaseous	267:273	arg1	evolution					283:291	gaseous product evolution	267:291	gaseous product evolution	267:291	The pyrolysis behavior, gaseous product evolution, kinetics and thermodynamics of these model compounds were investigated via TG-FTIR under high heating rates.					
30708332	1	60	theme	oil	182:184	arg1	behavior					163:170	the pyrolysis behavior	149:170	the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose	149:240	The aim of this work was to study the pyrolysis behavior of castor oil, corn starch, soy protein, lignin, xylan, and cellulose.					
31030410	7	0	theme	cell	1150:1153	arg1	assay					1169:1173	cell proliferation assay	1150:1173	cell proliferation assay	1150:1173	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	4	1	from	groups	825:830	arg1	EPSs					841:844	three EPSs	835:844	three EPSs	835:844	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	4	2	theme	FT-IR	721:725	arg1	analysis					736:743	FT-IR spectral analysis	721:743	FT-IR spectral analysis	721:743	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	6	3	theme	shape	1021:1025	arg1	form					1027:1030	nearly globular shape form	1005:1030	nearly globular shape form in aqueous solution	1005:1050	However, all EPSs molecule existed as nearly globular shape form in aqueous solution.					
31030410	2	4	theme	EPS	543:545	arg1	yield					547:551	the EPS yield	539:551	the EPS yield	539:551	Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield.					
31030410	6	5	from	form	1027:1030	arg1	solution					1043:1050	aqueous solution	1035:1050	aqueous solution	1035:1050	However, all EPSs molecule existed as nearly globular shape form in aqueous solution.					
31030410	1	6	theme	different	179:187	arg1	sources					202:208	different three carbon sources	179:208	different three carbon sources	179:208	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	6	theme	different	179:187	arg1	fructose					229:236	fructose	229:236	fructose	229:236	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	6	theme	different	179:187	arg1	sucrose					243:249	sucrose	243:249	sucrose	243:249	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	6	theme	different	179:187	arg1	glucose					220:226	glucose	220:226	glucose	220:226	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	3	7	theme	monosaccharide	652:665	arg1	different					696:704	different	696:704	different	696:704	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	7	theme	monosaccharide	652:665	arg1	composition					667:677	the monosaccharide composition	648:677	the monosaccharide composition of EPSs	648:685	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	0	8	theme	areolatum	117:125	arg1	Ehrenb					127:132	Scleroderma areolatum Ehrenb	105:132	Scleroderma areolatum Ehrenb	105:132	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	0	9	theme	Scleroderma	105:115	arg1	Ehrenb					127:132	Scleroderma areolatum Ehrenb	105:132	Scleroderma areolatum Ehrenb	105:132	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	8	10	theme	molecular	1327:1335	arg1	weight					1337:1342	low molecular weight	1323:1342	low molecular weight	1323:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	1	11	from	effects	168:174	arg1	properties					277:286	molecular properties	267:286	molecular properties	267:286	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	11	from	effects	168:174	arg1	activity					310:317	antiproliferative activity	292:317	antiproliferative activity of exopolysaccharide (EPS)	292:344	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	11	from	effects	168:174	arg1	production					255:264	production	255:264	production	255:264	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	4	12	theme	characteristic	810:823	arg1	groups					825:830	the prominent characteristic groups	796:830	the prominent characteristic groups in three EPSs	796:844	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	5	13	theme	carbon	897:902	arg1	source					904:909	carbon source	897:909	carbon source	897:909	The molecular weight of EPSs was also affected by carbon source, being generally lower compared with that with glucose.					
31030410	4	14	theme	prominent	800:808	arg1	groups					825:830	the prominent characteristic groups	796:830	the prominent characteristic groups in three EPSs	796:844	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	1	15	theme	carbon	195:200	arg1	sources					202:208	different three carbon sources	179:208	different three carbon sources	179:208	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	15	theme	carbon	195:200	arg1	fructose					229:236	fructose	229:236	fructose	229:236	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	15	theme	carbon	195:200	arg1	sucrose					243:249	sucrose	243:249	sucrose	243:249	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	15	theme	carbon	195:200	arg1	glucose					220:226	glucose	220:226	glucose	220:226	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	8	16	theme	antiproliferative	1225:1241	arg1	activity					1243:1250	higher antiproliferative activity	1218:1250	higher antiproliferative activity	1218:1250	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	7	17	dep	affected	1090:1097	arg1	examined					1126:1133	examined	1126:1133	affected antiproliferative activity examined in vitro using cell proliferation assay	1090:1173	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	7	18	theme	antiproliferative	1099:1115	arg1	activity					1117:1124	antiproliferative activity	1099:1124	antiproliferative activity	1099:1124	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	8	19	with	EPS	1314:1316	arg1	weight					1337:1342	low molecular weight	1323:1342	low molecular weight	1323:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	1	20	theme	sources	202:208	arg1	effects					168:174	The effects	164:174	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS),	164:345	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	8	21	theme	higher	1218:1223	arg1	activity					1243:1250	higher antiproliferative activity	1218:1250	higher antiproliferative activity	1218:1250	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	3	22	theme	predominant	567:577	arg1	acid					630:633	gluconic acid	621:633	gluconic acid	621:633	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	22	theme	predominant	567:577	arg1	compositions					592:603	the predominant carbohydrate compositions	563:603	the predominant carbohydrate compositions identified	563:614	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	7	23	theme	proliferation	1155:1167	arg1	assay					1169:1173	cell proliferation assay	1150:1173	cell proliferation assay	1150:1173	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	5	24	theme	molecular	851:859	arg1	weight					861:866	The molecular weight	847:866	The molecular weight of EPSs	847:874	The molecular weight of EPSs was also affected by carbon source, being generally lower compared with that with glucose.					
31030410	1	25	theme	molecular	267:275	arg1	properties					277:286	molecular properties	267:286	molecular properties	267:286	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	25	theme	molecular	267:275	arg1	production					255:264	production	255:264	production	255:264	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	0	26	theme	carbon	149:154	arg1	source					156:161	different carbon source	139:161	different carbon source	139:161	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	2	27	theme	carbon	426:431	arg1	sources					433:439	carbon sources	426:439	carbon sources examined	426:448	Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield.					
31030410	6	28	theme	EPSs	980:983	arg1	molecule					985:992	all EPSs molecule	976:992	all EPSs molecule	976:992	However, all EPSs molecule existed as nearly globular shape form in aqueous solution.					
31030410	0	29	theme	different	139:147	arg1	source					156:161	different carbon source	139:161	different carbon source	139:161	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	1	30	theme	submerged	369:377	arg1	culture					379:385	the submerged culture	365:385	the submerged culture of Scleroderma areolatum Ehrenb	365:417	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	4	31	theme	significant	767:777	arg1	difference					779:788	no significant difference	764:788	no significant difference	764:788	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	5	32	theme	EPSs	871:874	arg1	weight					861:866	The molecular weight	847:866	The molecular weight of EPSs	847:874	The molecular weight of EPSs was also affected by carbon source, being generally lower compared with that with glucose.					
31030410	2	33	theme	sucrose	467:473	arg1	addition					455:462	the addition	451:462	the addition of sucrose	451:473	Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield.					
31030410	8	34	theme	low	1323:1325	arg1	weight					1337:1342	low molecular weight	1323:1342	low molecular weight	1323:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	7	35	theme	carbon	1070:1075	arg1	sources					1077:1083	carbon sources	1070:1083	carbon sources	1070:1083	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	1	36	theme	antiproliferative	292:308	arg1	activity					310:317	antiproliferative activity	292:317	antiproliferative activity of exopolysaccharide (EPS)	292:344	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	36	theme	antiproliferative	292:308	arg1	production					255:264	production	255:264	production	255:264	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	6	37	theme	globular	1012:1019	arg1	form					1027:1030	nearly globular shape form	1005:1030	nearly globular shape form in aqueous solution	1005:1050	However, all EPSs molecule existed as nearly globular shape form in aqueous solution.					
31030410	8	38	theme	carbon	1197:1202	arg1	source					1204:1209	optimal carbon source	1189:1209	optimal carbon source	1189:1209	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	1	39	theme	Scleroderma	390:400	arg1	Ehrenb					412:417	Scleroderma areolatum Ehrenb	390:417	Scleroderma areolatum Ehrenb	390:417	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	3	40	theme	carbohydrate	579:590	arg1	acid					630:633	gluconic acid	621:633	gluconic acid	621:633	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	40	theme	carbohydrate	579:590	arg1	compositions					592:603	the predominant carbohydrate compositions	563:603	the predominant carbohydrate compositions identified	563:614	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	7	41	theme	sources	1077:1083	arg1	variation					1057:1065	The variation	1053:1065	The variation of carbon sources	1053:1083	The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay.					
31030410	0	42	theme	antiproliferative	45:61	arg1	activity					63:70	antiproliferative activity	45:70	antiproliferative activity	45:70	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	1	43	theme	areolatum	402:410	arg1	Ehrenb					412:417	Scleroderma areolatum Ehrenb	390:417	Scleroderma areolatum Ehrenb	390:417	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	8	44	from	contents	1288:1295	arg1	EPS					1314:1316	the EPS	1310:1316	the EPS with low molecular weight	1310:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	4	45	theme	spectral	727:734	arg1	analysis					736:743	FT-IR spectral analysis	721:743	FT-IR spectral analysis	721:743	FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs.					
31030410	1	46	theme	exopolysaccharide	322:338	arg1	properties					277:286	molecular properties	267:286	molecular properties	267:286	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	46	theme	exopolysaccharide	322:338	arg1	activity					310:317	antiproliferative activity	292:317	antiproliferative activity of exopolysaccharide (EPS)	292:344	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	46	theme	exopolysaccharide	322:338	arg1	production					255:264	production	255:264	production	255:264	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	1	47	theme	Ehrenb	412:417	arg1	culture					379:385	the submerged culture	365:385	the submerged culture of Scleroderma areolatum Ehrenb	365:417	The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb.					
31030410	8	48	theme	xylose	1300:1305	arg1	contents					1288:1295	the relatively high contents	1268:1295	the relatively high contents of xylose in the EPS with low molecular weight	1268:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	6	49	theme	aqueous	1035:1041	arg1	solution					1043:1050	aqueous solution	1035:1050	aqueous solution	1035:1050	However, all EPSs molecule existed as nearly globular shape form in aqueous solution.					
31030410	0	50	theme	exopolysaccharide	75:91	arg1	Production					0:9	Production	0:9	Production	0:9	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	0	50	theme	exopolysaccharide	75:91	arg1	characterization					23:38	structural characterization	12:38	structural characterization	12:38	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	0	50	theme	exopolysaccharide	75:91	arg1	activity					63:70	antiproliferative activity	45:70	antiproliferative activity	45:70	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	3	51	theme	EPSs	682:685	arg1	different					696:704	different	696:704	different	696:704	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	51	theme	EPSs	682:685	arg1	composition					667:677	the monosaccharide composition	648:677	the monosaccharide composition of EPSs	648:685	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	52	theme	gluconic	621:628	arg1	acid					630:633	gluconic acid	621:633	gluconic acid	621:633	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	3	52	theme	gluconic	621:628	arg1	compositions					592:603	the predominant carbohydrate compositions	563:603	the predominant carbohydrate compositions identified	563:614	Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly.					
31030410	8	53	theme	high	1283:1286	arg1	contents					1288:1295	the relatively high contents	1268:1295	the relatively high contents of xylose in the EPS with low molecular weight	1268:1342	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31030410	2	54	theme	mycelia	489:495	arg1	production					497:506	the mycelia production	485:506	the mycelia production	485:506	Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield.					
31030410	0	55	theme	structural	12:21	arg1	characterization					23:38	structural characterization	12:38	structural characterization	12:38	Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source.					
31030410	8	56	theme	optimal	1189:1195	arg1	source					1204:1209	optimal carbon source	1189:1209	optimal carbon source	1189:1209	Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.					
31608625	0	0	theme	Heparan	99:105	arg1	Sulfate					107:113	Heparan Sulfate	99:113	Heparan Sulfate	99:113	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	9	1	theme	biological	1455:1464	arg1	sources					1466:1472	biological sources	1455:1472	biological sources	1455:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	4	2	from	end	654:656	arg1	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	5	3	from	preparation	820:830	arg1	scale					948:952	a milligram scale	936:952	a milligram scale	936:952	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	2	4	theme	glucosamine	374:384	arg1	3-sulfotransferases					386:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	The 3-O-sulfated glucosamine is biosynthesized by heparan sulfate glucosamine 3-sulfotransferases.					
31608625	6	5	theme	synthetic	1055:1063	arg1	octasaccharides					1065:1079	synthetic octasaccharides	1055:1079	synthetic octasaccharides using heparin lyases	1055:1100	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	7	6	from	study	1248:1252	arg1	findings					1223:1230	the findings	1219:1230	the findings from a previous study	1219:1252	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	4	7	theme	nonreducing	642:652	arg1	due					668:670	due	668:670	due	668:670	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	4	7	theme	nonreducing	642:652	arg1	end					654:656	the nonreducing end	638:656	the nonreducing end	638:656	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	0	8	theme	Sulfate	107:113	arg1	Analysis					87:94	Compositional Analysis	73:94	Compositional Analysis of Heparan Sulfate	73:113	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	6	9	theme	3-O-sulfated	972:983	arg1	standards					1018:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	1	10	theme	thrombotic	273:282	arg1	disorders					284:292	thrombotic disorders	273:292	thrombotic disorders in hospitals	273:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	4	11	theme	analysis	763:770	arg1	procedure					772:780	the compositional analysis procedure	745:780	the compositional analysis procedure	745:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	7	12	theme	basic	1190:1194	arg1	conditions					1196:1205	basic conditions	1190:1205	basic conditions	1190:1205	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	5	13	theme	disaccharide	852:863	arg1	preparation					820:830	the preparation	816:830	the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale	816:952	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	4	14	contain	contain	582:588	arg2	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	4	14	contain	contain	582:588	arg1	oligosaccharides					560:575	These oligosaccharides	554:575	These oligosaccharides	554:575	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	3	15	theme	3-O-sulfated	467:478	arg1	standards					496:504	3-O-sulfated oligosaccharide standards	467:504	3-O-sulfated oligosaccharide standards	467:504	Because of its biological significance, there is a need for 3-O-sulfated oligosaccharide standards to facilitate the compositional analysis of HS.					
31608625	8	16	theme	unwanted	1259:1266	arg1	degradation					1268:1278	The unwanted degradation	1255:1278	The unwanted degradation	1255:1278	The unwanted degradation was reduced by decreasing the pH in the presence of phosphate buffer.					
31608625	5	17	theme	milligram	938:946	arg1	scale					948:952	a milligram scale	936:952	a milligram scale	936:952	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	7	18	theme	previous	1239:1246	arg1	study					1248:1252	a previous study	1237:1252	a previous study	1237:1252	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	3	19	theme	oligosaccharide	480:494	arg1	standards					496:504	3-O-sulfated oligosaccharide standards	467:504	3-O-sulfated oligosaccharide standards	467:504	Because of its biological significance, there is a need for 3-O-sulfated oligosaccharide standards to facilitate the compositional analysis of HS.					
31608625	3	20	theme	biological	422:431	arg1	significance					433:444	its biological significance	418:444	its biological significance	418:444	Because of its biological significance, there is a need for 3-O-sulfated oligosaccharide standards to facilitate the compositional analysis of HS.					
31608625	4	21	theme	ΔUA	622:624	arg1	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	1	22	from	disorders	284:292	arg1	hospitals					297:305	hospitals	297:305	hospitals	297:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	8	23	dep	buffer	1342:1347	arg1	presence					1320:1327	presence	1320:1327	presence	1320:1327	The unwanted degradation was reduced by decreasing the pH in the presence of phosphate buffer.					
31608625	8	23	dep	buffer	1342:1347	arg1	the					1316:1318	the	1316:1318	the	1316:1318	The unwanted degradation was reduced by decreasing the pH in the presence of phosphate buffer.					
31608625	4	24	theme	heparin	718:724	arg1	lyases					726:731	heparin lyases	718:731	heparin lyases used during the compositional analysis procedure	718:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	8	25	theme	phosphate	1332:1340	arg1	buffer					1342:1347	phosphate buffer	1332:1347	phosphate buffer	1332:1347	The unwanted degradation was reduced by decreasing the pH in the presence of phosphate buffer.					
31608625	0	26	theme	Disaccharide	26:37	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide	0:57	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	4	27	theme	acid	616:619	arg1	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	4	28	theme	compositional	749:761	arg1	procedure					772:780	the compositional analysis procedure	745:780	the compositional analysis procedure	745:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	2	29	theme	3-O-sulfated	312:323	arg1	glucosamine					325:335	The 3-O-sulfated glucosamine	308:335	The 3-O-sulfated glucosamine	308:335	The 3-O-sulfated glucosamine is biosynthesized by heparan sulfate glucosamine 3-sulfotransferases.					
31608625	0	30	theme	3-O-Sulfated	13:24	arg1	Disaccharide					26:37	3-O-Sulfated Disaccharide	13:37	3-O-Sulfated Disaccharide	13:37	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	6	31	theme	standards	1018:1026	arg1	synthesis					959:967	The synthesis	955:967	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards	955:1026	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	3	32	theme	HS	550:551	arg1	analysis					538:545	the compositional analysis	520:545	the compositional analysis of HS	520:551	Because of its biological significance, there is a need for 3-O-sulfated oligosaccharide standards to facilitate the compositional analysis of HS.					
31608625	1	33	theme	various	197:203	arg1	activity					255:262	the anticoagulant activity	237:262	the anticoagulant activity to treat thrombotic disorders in hospitals	237:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	33	theme	various	197:203	arg1	functions					216:224	various biological functions	197:224	various biological functions	197:224	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	0	34	theme	Tetrasaccharide	43:57	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide	0:57	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	2	35	theme	heparan	358:364	arg1	3-sulfotransferases					386:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	The 3-O-sulfated glucosamine is biosynthesized by heparan sulfate glucosamine 3-sulfotransferases.					
31608625	7	36	theme	3-O-sulfated	1134:1145	arg1	labile					1177:1182	labile	1177:1182	labile	1177:1182	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	7	36	theme	3-O-sulfated	1134:1145	arg1	standards					1163:1171	3-O-sulfated oligosaccharide standards	1134:1171	3-O-sulfated oligosaccharide standards	1134:1171	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	4	37	theme	depolymerization	679:694	arg1	reaction					696:703	the depolymerization reaction	675:703	the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	675:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	6	38	theme	tetrasaccharide	1002:1016	arg1	standards					1018:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	1	39	theme	biological	205:214	arg1	activity					255:262	the anticoagulant activity	237:262	the anticoagulant activity to treat thrombotic disorders in hospitals	237:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	39	theme	biological	205:214	arg1	functions					216:224	various biological functions	197:224	various biological functions	197:224	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	5	40	theme	tetrasaccharides	901:916	arg1	preparation					820:830	the preparation	816:830	the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale	816:952	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	7	41	theme	oligosaccharide	1147:1161	arg1	labile					1177:1182	labile	1177:1182	labile	1177:1182	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	7	41	theme	oligosaccharide	1147:1161	arg1	standards					1163:1171	3-O-sulfated oligosaccharide standards	1134:1171	3-O-sulfated oligosaccharide standards	1134:1171	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	1	42	attach	linked	187:192	arg2	3-O-Sulfation					116:128	3-O-Sulfation	116:128	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS)	116:182	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	42	attach	linked	187:192	arg1	activity					255:262	the anticoagulant activity	237:262	the anticoagulant activity to treat thrombotic disorders in hospitals	237:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	42	attach	linked	187:192	arg1	functions					216:224	various biological functions	197:224	various biological functions	197:224	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	5	43	theme	3-O-sulfated	888:899	arg1	compound					919:926	compound 1-3	919:930	compound 1-3	919:930	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	5	43	theme	3-O-sulfated	888:899	arg1	tetrasaccharides					901:916	three 3-O-sulfated tetrasaccharides	882:916	three 3-O-sulfated tetrasaccharides (compound 1-3)	882:931	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	6	44	theme	disaccharide	985:996	arg1	standards					1018:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	3-O-sulfated disaccharide and tetrasaccharide standards	972:1026	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	9	45	attach	derived	1442:1448	arg2	HS					1439:1440	HS	1439:1440	HS derived from biological sources	1439:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	9	45	attach	derived	1442:1448	arg1	sources					1466:1472	biological sources	1455:1472	biological sources	1455:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	2	46	theme	sulfate	366:372	arg1	3-sulfotransferases					386:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	heparan sulfate glucosamine 3-sulfotransferases	358:404	The 3-O-sulfated glucosamine is biosynthesized by heparan sulfate glucosamine 3-sulfotransferases.					
31608625	5	47	theme	3-O-sulfated	839:850	arg1	compound					866:873	compound 4	866:875	compound 4	866:875	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	5	47	theme	3-O-sulfated	839:850	arg1	disaccharide					852:863	one 3-O-sulfated disaccharide	835:863	one 3-O-sulfated disaccharide (compound 4)	835:876	Here, we describe a protocol for the preparation of one 3-O-sulfated disaccharide (compound 4) and three 3-O-sulfated tetrasaccharides (compound 1-3) in a milligram scale.					
31608625	4	48	theme	uronic	609:614	arg1	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	1	49	theme	glucosamine	137:147	arg1	unit					155:158	the glucosamine sugar unit	133:158	the glucosamine sugar unit	133:158	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	50	from	3-O-Sulfation	116:128	arg1	HS					180:181	HS	180:181	HS	180:181	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	50	from	3-O-Sulfation	116:128	arg1	unit					155:158	the glucosamine sugar unit	133:158	the glucosamine sugar unit	133:158	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	50	from	3-O-Sulfation	116:128	arg1	sulfate					171:177	heparan sulfate	163:177	heparan sulfate (HS)	163:182	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	4	51	theme	Δ4,5-unsaturated	592:607	arg1	residue					627:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue	590:633	a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure	590:780	These oligosaccharides must contain a Δ4,5-unsaturated uronic acid (ΔUA) residue at the nonreducing end, which is due to the depolymerization reaction catalyzed by heparin lyases used during the compositional analysis procedure.					
31608625	7	52	theme	Further	1103:1109	arg1	analysis					1111:1118	Further analysis	1103:1118	Further analysis	1103:1118	Further analysis revealed that 3-O-sulfated oligosaccharide standards are labile under basic conditions, confirming the findings from a previous study.					
31608625	1	53	theme	sugar	149:153	arg1	unit					155:158	the glucosamine sugar unit	133:158	the glucosamine sugar unit	133:158	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	9	54	theme	3-O-sulfated	1354:1365	arg1	reagents					1397:1404	reagents	1397:1404	reagents to characterize 3-O-sulfation in HS derived from biological sources	1397:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	9	54	theme	3-O-sulfated	1354:1365	arg1	standards					1383:1391	The 3-O-sulfated oligosaccharide standards	1350:1391	The 3-O-sulfated oligosaccharide standards	1350:1391	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	3	55	theme	compositional	524:536	arg1	analysis					538:545	the compositional analysis	520:545	the compositional analysis of HS	520:551	Because of its biological significance, there is a need for 3-O-sulfated oligosaccharide standards to facilitate the compositional analysis of HS.					
31608625	9	56	theme	oligosaccharide	1367:1381	arg1	reagents					1397:1404	reagents	1397:1404	reagents to characterize 3-O-sulfation in HS derived from biological sources	1397:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	9	56	theme	oligosaccharide	1367:1381	arg1	standards					1383:1391	The 3-O-sulfated oligosaccharide standards	1350:1391	The 3-O-sulfated oligosaccharide standards	1350:1391	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	6	57	theme	heparin	1087:1093	arg1	lyases					1095:1100	heparin lyases	1087:1100	heparin lyases	1087:1100	The synthesis of 3-O-sulfated disaccharide and tetrasaccharide standards was completed by degrading synthetic octasaccharides using heparin lyases.					
31608625	0	58	theme	Compositional	73:85	arg1	Analysis					87:94	Compositional Analysis	73:94	Compositional Analysis of Heparan Sulfate	73:113	Synthesis of 3-O-Sulfated Disaccharide and Tetrasaccharide Standards for Compositional Analysis of Heparan Sulfate.					
31608625	9	59	from	3-O-sulfation	1422:1434	arg1	HS					1439:1440	HS	1439:1440	HS derived from biological sources	1439:1472	The 3-O-sulfated oligosaccharide standards are reagents to characterize 3-O-sulfation in HS derived from biological sources.					
31608625	1	60	theme	heparan	163:169	arg1	HS					180:181	HS	180:181	HS	180:181	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	60	theme	heparan	163:169	arg1	sulfate					171:177	heparan sulfate	163:177	heparan sulfate (HS)	163:182	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
31608625	1	61	theme	anticoagulant	241:253	arg1	activity					255:262	the anticoagulant activity	237:262	the anticoagulant activity to treat thrombotic disorders in hospitals	237:305	3-O-Sulfation on the glucosamine sugar unit in heparan sulfate (HS) is linked to various biological functions, including the anticoagulant activity to treat thrombotic disorders in hospitals.					
30130389	0	0	theme	Ionic	92:96	arg1	Liquids					98:104	Ionic Liquids	92:104	Ionic Liquids	92:104	A Hierarchical Model To Understand the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids.					
30130389	2	1	from	dissolution	460:470	arg1	regeneration					492:503	regeneration	492:503	regeneration	492:503	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	2	1	from	dissolution	460:470	arg1	liquid					481:486	liquid	481:486	liquid	481:486	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	7	2	dep	shows	1411:1415	arg1	exposed					1429:1435	exposed	1429:1435	shows differently exposed surface area depending on the blend	1411:1471	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	1	3	theme	renewable	155:163	arg1	sources					165:171	abundant and renewable sources	142:171	abundant and renewable sources	142:171	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	9	4	theme	Young	1727:1731	arg1	modulus					1735:1741	the Young's modulus	1723:1741	the Young's modulus	1723:1741	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	2	5	from	liquid	481:486	arg1	water					508:512	water	508:512	water	508:512	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	10	6	theme	experimental	1812:1823	arg1	trends					1825:1830	experimental trends	1812:1830	experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend	1812:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	8	7	theme	differential	1483:1494	arg1	DSC					1518:1520	DSC	1518:1520	DSC	1518:1520	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	8	7	theme	differential	1483:1494	arg1	calorimetry					1505:1515	differential scanning calorimetry	1483:1515	differential scanning calorimetry (DSC)	1483:1521	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	3	8	theme	theoretical	534:544	arg1	model					546:550	a novel theoretical model	526:550	a novel theoretical model that correlates the composite's microscopic structure to its bulk properties	526:627	We propose a novel theoretical model that correlates the composite's microscopic structure to its bulk properties.					
30130389	4	9	theme	biomolecules	828:839	arg1	mixture					811:817	a mixture	809:817	a mixture of these biomolecules	809:839	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	1	10	from	sources	165:171	arg1	biomaterials					124:135	biomaterials	124:135	biomaterials from abundant and renewable sources	124:171	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	2	11	theme	molecular	283:291	arg1	simulations					302:312	molecular dynamics simulations	283:312	molecular dynamics simulations	283:312	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	1	12	theme	recent	110:115	arg1	years					117:121	recent years	110:121	recent years	110:121	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	6	13	theme	biocomposites	1199:1211	arg1	temperature					1172:1182	the decomposition temperature	1154:1182	the decomposition temperature of the blended biocomposites compared to their pure counterparts	1154:1247	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	10	14	from	concentration	1883:1895	arg1	blend					1923:1927	the biopolymer blend	1908:1927	the biopolymer blend	1908:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	5	15	theme	biopolymer	996:1005	arg1	blend					1007:1011	the biopolymer blend	992:1011	the biopolymer blend	992:1011	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	3	16	theme	novel	528:532	arg1	model					546:550	a novel theoretical model	526:550	a novel theoretical model that correlates the composite's microscopic structure to its bulk properties	526:627	We propose a novel theoretical model that correlates the composite's microscopic structure to its bulk properties.					
30130389	4	17	theme	thermal	771:777	arg1	properties					795:804	structural, thermal, and mechanical properties	759:804	structural, thermal, and mechanical properties of a mixture of these biomolecules	759:839	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	0	18	from	Processing	39:48	arg1	Liquids					98:104	Ionic Liquids	92:104	Ionic Liquids	92:104	A Hierarchical Model To Understand the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids.					
30130389	7	19	theme	scanning	1372:1379	arg1	microscopy					1390:1399	scanning electron microscopy	1372:1399	scanning electron microscopy (SEM)	1372:1405	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	7	19	theme	scanning	1372:1379	arg1	SEM					1402:1404	SEM	1402:1404	SEM	1402:1404	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	5	20	theme	blend	1007:1011	arg1	crystallinity					975:987	crystallinity	975:987	crystallinity of the biopolymer blend	975:1011	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	5	21	theme	pure	900:903	arg1	substance					905:913	the pure substance	896:913	the pure substance	896:913	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	5	22	dep	spectroscopy	1085:1096	arg1	ATR-FTIR					1099:1106	ATR-FTIR	1099:1106	ATR-FTIR	1099:1106	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	2	23	from	water	508:512	arg1	liquid					481:486	liquid	481:486	liquid	481:486	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	2	24	theme	theoretical	261:271	arg1	modeling					273:280	theoretical modeling	261:280	theoretical modeling	261:280	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	2	25	theme	chitin/silk-	402:413	arg1	regeneration					369:380	the regeneration	365:380	the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water	365:512	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	0	26	theme	Hierarchical	2:13	arg1	Model					15:19	A Hierarchical Model	0:19	A Hierarchical Model	0:19	A Hierarchical Model To Understand the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids.					
30130389	8	27	from	process	1638:1644	arg1	water					1649:1653	water	1649:1653	water	1649:1653	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	4	28	theme	mechanical	784:793	arg1	properties					795:804	structural, thermal, and mechanical properties	759:804	structural, thermal, and mechanical properties of a mixture of these biomolecules	759:839	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	7	29	theme	electron	1381:1388	arg1	microscopy					1390:1399	scanning electron microscopy	1372:1399	scanning electron microscopy (SEM)	1372:1405	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	7	29	theme	electron	1381:1388	arg1	SEM					1402:1404	SEM	1402:1404	SEM	1402:1404	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	5	30	dep	transform	1066:1074	arg1	infrared					1076:1083	infrared	1076:1083	transform infrared spectroscopy (ATR-FTIR)	1066:1107	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	10	31	theme	Young	1848:1852	arg1	modulus					1856:1862	the Young's modulus	1844:1862	the Young's modulus due to varying the concentration of silk in the biopolymer blend	1844:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	6	32	theme	blended	1191:1197	arg1	biocomposites					1199:1211	the blended biocomposites	1187:1211	the blended biocomposites	1187:1211	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	2	33	theme	cellulose/silk-	385:399	arg1	regeneration					369:380	the regeneration	365:380	the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water	365:512	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	6	34	theme	Thermogravimetric	1110:1126	arg1	TGA					1138:1140	TGA	1138:1140	TGA	1138:1140	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	6	34	theme	Thermogravimetric	1110:1126	arg1	analysis					1128:1135	Thermogravimetric analysis	1110:1135	Thermogravimetric analysis (TGA)	1110:1141	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	9	35	theme	model	1679:1683	arg1	prediction					1709:1718	our model's prediction	1697:1718	our model's prediction of the Young's modulus	1697:1741	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	9	35	theme	model	1679:1683	arg1	test					1667:1670	a final test	1659:1670	a final test of the model	1659:1683	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	8	36	theme	regeneration	1625:1636	arg1	process					1638:1644	the regeneration process	1621:1644	the regeneration process in water	1621:1653	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	3	37	theme	microscopic	584:594	arg1	structure					596:604	the composite's microscopic structure	568:604	the composite's microscopic structure to its bulk properties	568:627	We propose a novel theoretical model that correlates the composite's microscopic structure to its bulk properties.					
30130389	6	38	theme	theoretical	1283:1293	arg1	predictions					1295:1305	our theoretical predictions	1279:1305	our theoretical predictions	1279:1305	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	6	39	theme	pure	1231:1234	arg1	counterparts					1236:1247	their pure counterparts	1225:1247	their pure counterparts	1225:1247	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	8	40	theme	scanning	1496:1503	arg1	DSC					1518:1520	DSC	1518:1520	DSC	1518:1520	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	8	40	theme	scanning	1496:1503	arg1	calorimetry					1505:1515	differential scanning calorimetry	1483:1515	differential scanning calorimetry (DSC)	1483:1521	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	4	41	link	non-cross-linked	650:665	arg1	biopolymers					667:677	modeling non-cross-linked biopolymers	641:677	modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets	641:729	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	2	42	theme	dynamics	293:300	arg1	simulations					302:312	molecular dynamics simulations	283:312	molecular dynamics simulations	283:312	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	2	43	theme	biocomposites	440:452	arg1	regeneration					369:380	the regeneration	365:380	the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water	365:512	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	6	44	theme	decomposition	1158:1170	arg1	temperature					1172:1182	the decomposition temperature	1154:1182	the decomposition temperature of the blended biocomposites compared to their pure counterparts	1154:1247	Thermogravimetric analysis (TGA) shows that the decomposition temperature of the blended biocomposites compared to their pure counterparts is reduced in accordance with our theoretical predictions.					
30130389	4	45	theme	layer-like	692:701	arg1	structures					703:712	layer-like structures	692:712	layer-like structures such as β-sheets	692:729	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	4	45	theme	layer-like	692:701	arg1	β-sheets					722:729	β-sheets	722:729	β-sheets	722:729	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	5	46	theme	total	1040:1044	arg1	Fourier					1058:1064	attenuated total reflectance Fourier	1029:1064	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR)	1029:1107	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	2	47	theme	chitosan/silk-based	420:438	arg1	biocomposites					440:452	chitosan/silk-based biocomposites	420:452	chitosan/silk-based biocomposites	420:452	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	5	48	dep	Fourier	1058:1064	arg1	transform					1066:1074	transform	1066:1074	transform infrared spectroscopy (ATR-FTIR)	1066:1107	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	9	49	theme	modulus	1735:1741	arg1	prediction					1709:1718	our model's prediction	1697:1718	our model's prediction of the Young's modulus	1697:1741	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	9	49	theme	modulus	1735:1741	arg1	test					1667:1670	a final test	1659:1670	a final test of the model	1659:1683	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	5	50	theme	reflectance	1046:1056	arg1	Fourier					1058:1064	attenuated total reflectance Fourier	1029:1064	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR)	1029:1107	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	1	51	theme	science	220:226	arg1	alike					228:232	science alike	220:232	science alike	220:232	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	5	52	theme	chosen	922:927	arg1	solvent					929:935	the chosen solvent	918:935	the chosen solvent	918:935	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	8	53	theme	essential	1596:1604	arg1	content					1571:1577	the residual water content	1552:1577	the residual water content	1552:1577	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	3	54	theme	bulk	613:616	arg1	properties					618:627	its bulk properties	609:627	its bulk properties	609:627	We propose a novel theoretical model that correlates the composite's microscopic structure to its bulk properties.					
30130389	7	55	theme	material	1330:1337	arg1	morphology					1312:1321	The morphology	1308:1321	The morphology of the material	1308:1337	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	8	56	theme	water	1565:1569	arg1	content					1571:1577	the residual water content	1552:1577	the residual water content	1552:1577	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	7	57	theme	surface	1437:1443	arg1	area					1445:1448	surface area	1437:1448	surface area	1437:1448	The morphology of the material is further characterized through scanning electron microscopy (SEM) and shows differently exposed surface area depending on the blend.					
30130389	10	58	theme	biopolymer	1912:1921	arg1	blend					1923:1927	the biopolymer blend	1908:1927	the biopolymer blend	1908:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	4	59	theme	structural	759:768	arg1	properties					795:804	structural, thermal, and mechanical properties	759:804	structural, thermal, and mechanical properties of a mixture of these biomolecules	759:839	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	5	60	theme	attenuated	1029:1038	arg1	Fourier					1058:1064	attenuated total reflectance Fourier	1029:1064	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR)	1029:1107	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	10	61	theme	due	1864:1866	arg1	modulus					1856:1862	the Young's modulus	1844:1862	the Young's modulus due to varying the concentration of silk in the biopolymer blend	1844:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	5	62	from	solubility	882:891	arg1	solvent					929:935	the chosen solvent	918:935	the chosen solvent	918:935	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	2	63	theme	experimental	327:338	arg1	techniques					340:349	several experimental techniques	319:349	several experimental techniques	319:349	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	5	64	theme	crystallinity	975:987	arg1	crystallinity					975:987	crystallinity	975:987	crystallinity of the biopolymer blend	975:1011	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	5	64	theme	crystallinity	975:987	arg1	amount					965:970	the amount	961:970	the amount of crystallinity of the biopolymer blend	961:1011	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	10	65	theme	silk	1900:1903	arg1	concentration					1883:1895	the concentration	1879:1895	the concentration of silk in the biopolymer blend	1879:1927	The model correctly reproduces experimental trends observed in the Young's modulus due to varying the concentration of silk in the biopolymer blend.					
30130389	4	66	theme	mixture	811:817	arg1	properties					795:804	structural, thermal, and mechanical properties	759:804	structural, thermal, and mechanical properties of a mixture of these biomolecules	759:839	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	2	67	theme	several	319:325	arg1	techniques					340:349	several experimental techniques	319:349	several experimental techniques	319:349	In this study, we combine theoretical modeling, molecular dynamics simulations, and several experimental techniques to understand the regeneration of cellulose/silk-, chitin/silk-, and chitosan/silk-based biocomposites after dissolution in ionic liquid and regeneration in water.					
30130389	0	68	theme	Films	83:87	arg1	Processing					39:48	the Processing	35:48	the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids	35:104	A Hierarchical Model To Understand the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids.					
30130389	1	69	dep	medicine	197:204	arg1	alike					228:232	science alike	220:232	science alike	220:232	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	1	70	theme	abundant	142:149	arg1	sources					165:171	abundant and renewable sources	142:171	abundant and renewable sources	142:171	In recent years, biomaterials from abundant and renewable sources have shown potential in medicine and materials science alike.					
30130389	5	71	theme	substance	905:913	arg1	solubility					882:891	the solubility	878:891	the solubility of the pure substance in the chosen solvent	878:935	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	4	72	theme	non-cross-linked	650:665	arg1	biopolymers					667:677	modeling non-cross-linked biopolymers	641:677	modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets	641:729	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30130389	0	73	theme	Polysaccharides/Protein-Based	53:81	arg1	Films					83:87	Polysaccharides/Protein-Based Films	53:87	Polysaccharides/Protein-Based Films	53:87	A Hierarchical Model To Understand the Processing of Polysaccharides/Protein-Based Films in Ionic Liquids.					
30130389	8	74	theme	residual	1556:1563	arg1	content					1571:1577	the residual water content	1552:1577	the residual water content	1552:1577	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	5	75	used	used	944:947	arg2	solubility					882:891	the solubility	878:891	the solubility of the pure substance in the chosen solvent	878:935	Our model and experiments show that the solubility of the pure substance in the chosen solvent can be used to modulate the amount of crystallinity of the biopolymer blend, as measured by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR).					
30130389	8	76	from	content	1571:1577	arg1	material					1586:1593	the material	1582:1593	the material	1582:1593	Finally, differential scanning calorimetry (DSC) is performed to characterize the residual water content in the material, essential for explaining the regeneration process in water.					
30130389	9	77	theme	final	1661:1665	arg1	prediction					1709:1718	our model's prediction	1697:1718	our model's prediction of the Young's modulus	1697:1741	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	9	77	theme	final	1661:1665	arg1	test					1667:1670	a final test	1659:1670	a final test of the model	1659:1683	As a final test of the model, we compare our model's prediction of the Young's modulus with existing data in the literature.					
30130389	4	78	theme	modeling	641:648	arg1	biopolymers					667:677	modeling non-cross-linked biopolymers	641:677	modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets	641:729	We rely on modeling non-cross-linked biopolymers that present layer-like structures such as β-sheets and we successfully predict structural, thermal, and mechanical properties of a mixture of these biomolecules.					
30373703	0	0	theme	extracellular	80:92	arg1	composition					101:111	extracellular matrix composition	80:111	extracellular matrix composition	80:111	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	9	1	theme	threshold	1487:1495	arg1	levels					1497:1502	minimum threshold levels	1479:1502	minimum threshold levels	1479:1502	These data suggest that HA and HCs can support cumulus expansion provided that they are present above minimum threshold levels.					
30373703	5	2	dep	expansion	872:880	arg1	gonadotrophin					908:920	9 h-post-human chorionic gonadotrophin	883:920	9 h-post-human chorionic gonadotrophin (hCG)	883:926	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	2	dep	expansion	872:880	arg1	hCG					923:925	hCG	923:925	hCG	923:925	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	7	3	theme	DM	1100:1101	arg1	COCs					1103:1106	DM COCs	1100:1106	DM COCs	1100:1106	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	8	4	theme	Control	1360:1366	arg1	samples					1368:1374	DM or Control samples	1354:1374	DM or Control samples	1354:1374	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	10	5	theme	C1galt1	1549:1555	arg1	ablation					1537:1544	oocyte-specific ablation	1521:1544	oocyte-specific ablation of C1galt1 and Mgat1	1521:1565	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	6	6	theme	HA	1001:1002	arg1	levels					991:996	the levels	987:996	the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group)	987:1083	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	5	7	theme	chorionic	898:906	arg1	gonadotrophin					908:920	9 h-post-human chorionic gonadotrophin	883:920	9 h-post-human chorionic gonadotrophin (hCG)	883:926	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	7	theme	chorionic	898:906	arg1	hCG					923:925	hCG	923:925	hCG	923:925	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	8	theme	h-post-human	885:896	arg1	gonadotrophin					908:920	9 h-post-human chorionic gonadotrophin	883:920	9 h-post-human chorionic gonadotrophin (hCG)	883:926	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	8	theme	h-post-human	885:896	arg1	hCG					923:925	hCG	923:925	hCG	923:925	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	4	9	theme	mutant	537:542	arg1	mouse					549:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	0	10	theme	matrix	94:99	arg1	composition					101:111	extracellular matrix composition	80:111	extracellular matrix composition	80:111	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	5	11	dep	gonadotrophin	908:920	arg1	mice					941:944	control n=3 mice	929:944	control n=3 mice	929:944	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	11	dep	gonadotrophin	908:920	arg1	n=3					950:952	DM n=3	947:952	DM n=3 per group	947:962	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	6	12	theme	COCs	1069:1072	arg1	group					1078:1082	12-25 COCs per group	1063:1082	12-25 COCs per group	1063:1082	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	6	12	theme	COCs	1069:1072	arg1	phosphorylated-SMAD1/5/8					1026:1049	phosphorylated-SMAD1/5/8	1026:1049	phosphorylated-SMAD1/5/8	1026:1049	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	4	13	theme	I	721:721	arg1	deletion					629:636	oocyte-specific deletion	613:636	oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1)	613:729	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	10	14	theme	Mgat1	1561:1565	arg1	ablation					1537:1544	oocyte-specific ablation	1521:1544	oocyte-specific ablation of C1galt1 and Mgat1	1521:1565	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	4	15	theme	β1,3-galactosyltransferase	648:673	arg1	deletion					629:636	oocyte-specific deletion	613:636	oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1)	613:729	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	6	16	theme	TSG-6	1016:1020	arg1	levels					991:996	the levels	987:996	the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group)	987:1083	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	10	17	theme	oocyte-specific	1521:1535	arg1	ablation					1537:1544	oocyte-specific ablation	1521:1544	oocyte-specific ablation of C1galt1 and Mgat1	1521:1565	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	2	18	theme	matrix	258:263	arg1	constituent					265:275	the major matrix constituent	248:275	the major matrix constituent	248:275	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	18	theme	matrix	258:263	arg1	Hyaluronan					231:240	Hyaluronan	231:240	Hyaluronan (HA)	231:245	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	5	19	theme	mare	821:824	arg1	PMSG					847:850	PMSG	847:850	PMSG	847:850	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	19	theme	mare	821:824	arg1	gonadotrophin					832:844	48 h-post-pregnant mare serum gonadotrophin	802:844	48 h-post-pregnant mare serum gonadotrophin (PMSG)	802:851	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	4	20	theme	double	530:535	arg1	mouse					549:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	2	21	theme	major	252:256	arg1	constituent					265:275	the major matrix constituent	248:275	the major matrix constituent	248:275	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	21	theme	major	252:256	arg1	Hyaluronan					231:240	Hyaluronan	231:240	Hyaluronan (HA)	231:245	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	5	22	theme	h-post-pregnant	805:819	arg1	PMSG					847:850	PMSG	847:850	PMSG	847:850	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	22	theme	h-post-pregnant	805:819	arg1	gonadotrophin					832:844	48 h-post-pregnant mare serum gonadotrophin	802:844	48 h-post-pregnant mare serum gonadotrophin (PMSG)	802:851	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	7	23	theme	cell	1156:1159	arg1	density					1161:1167	cell density	1156:1167	cell density	1156:1167	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	10	24	theme	morphogenetic	1583:1595	arg1	synthesis					1608:1616	bone morphogenetic protein 15 synthesis	1578:1616	bone morphogenetic protein 15 synthesis	1578:1616	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	2	25	theme	inter-α-inhibitor	298:314	arg1	HCs					330:332	HCs	330:332	HCs	330:332	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	25	theme	inter-α-inhibitor	298:314	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	25	theme	inter-α-inhibitor	298:314	arg1	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	25	theme	inter-α-inhibitor	298:314	arg1	PTX3					349:352	pentraxin 3 (PTX3)	336:353	pentraxin 3 (PTX3)	336:353	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	7	26	theme	cumulus	1140:1146	arg1	size					1148:1151	cumulus size	1140:1151	cumulus size	1140:1151	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	4	27	theme	due	606:608	arg1	N-					589:590	complex N-	581:590	complex N-	581:590	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	0	28	theme	Oocyte-specific	0:14	arg1	ablation					16:23	Oocyte-specific ablation	0:23	Oocyte-specific ablation of N- and O-glycans	0:43	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	8	29	theme	cumulus	1338:1344	arg1	area					1346:1349	cumulus area	1338:1349	cumulus area	1338:1349	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	5	30	theme	serum	826:830	arg1	PMSG					847:850	PMSG	847:850	PMSG	847:850	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	30	theme	serum	826:830	arg1	gonadotrophin					832:844	48 h-post-pregnant mare serum gonadotrophin	802:844	48 h-post-pregnant mare serum gonadotrophin (PMSG)	802:851	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	0	31	theme	N-	28:29	arg1	ablation					16:23	Oocyte-specific ablation	0:23	Oocyte-specific ablation of N- and O-glycans	0:43	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	5	32	theme	control	929:935	arg1	mice					941:944	control n=3 mice	929:944	control n=3 mice	929:944	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	33	theme	late-stage	861:870	arg1	expansion					872:880	late-stage expansion	861:880	late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group)	861:963	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	4	34	theme	N-acetylglucosaminyltransferase	689:719	arg1	Mgat1					724:728	Mgat1	724:728	Mgat1	724:728	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	4	34	theme	N-acetylglucosaminyltransferase	689:719	arg1	I					721:721	N-acetylglucosaminyltransferase I	689:721	N-acetylglucosaminyltransferase I (Mgat1)	689:729	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	10	35	theme	HA	1681:1682	arg1	production					1684:1693	HA production	1681:1693	HA production	1681:1693	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	3	36	theme	oocyte-secreted	469:483	arg1	factors					485:491	oocyte-secreted factors	469:491	oocyte-secreted factors	469:491	All except HCs are secreted by cumulus cells in response to oocyte-secreted factors, which signal via SMAD pathways.					
30373703	1	37	theme	Cumulus-oocyte	114:127	arg1	COC					138:140	COC	138:140	COC	138:140	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	1	37	theme	Cumulus-oocyte	114:127	arg1	complex					129:135	Cumulus-oocyte complex	114:135	Cumulus-oocyte complex (COC) expansion	114:151	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	2	38	theme	factor-stimulated	375:391	arg1	TSG-6					401:405	TSG-6	401:405	TSG-6	401:405	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	38	theme	factor-stimulated	375:391	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	38	theme	factor-stimulated	375:391	arg1	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	0	39	theme	O-glycans	35:43	arg1	ablation					16:23	Oocyte-specific ablation	0:23	Oocyte-specific ablation of N- and O-glycans	0:43	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	2	40	attach	cross-linked	281:292	arg3	PTX3					349:352	pentraxin 3 (PTX3)	336:353	pentraxin 3 (PTX3)	336:353	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	40	attach	cross-linked	281:292	arg3	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	40	attach	cross-linked	281:292	arg3	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	40	attach	cross-linked	281:292	arg3	HCs					330:332	HCs	330:332	HCs	330:332	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	10	41	theme	SMAD1/5/8	1651:1659	arg1	phosphorylation					1661:1675	SMAD1/5/8 phosphorylation	1651:1675	SMAD1/5/8 phosphorylation	1651:1675	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	4	42	theme	complex	581:587	arg1	N-					589:590	complex N-	581:590	complex N-	581:590	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	5	43	theme	n=3	937:939	arg1	mice					941:944	control n=3 mice	929:944	control n=3 mice	929:944	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	2	44	theme	necrosis	366:373	arg1	TSG-6					401:405	TSG-6	401:405	TSG-6	401:405	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	44	theme	necrosis	366:373	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	44	theme	necrosis	366:373	arg1	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	4	45	theme	core	641:644	arg1	C1galt1					676:682	C1galt1	676:682	C1galt1	676:682	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	4	45	theme	core	641:644	arg1	β1,3-galactosyltransferase					648:673	core 1 β1,3-galactosyltransferase	641:673	core 1 β1,3-galactosyltransferase (C1galt1)	641:683	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	2	46	theme	tumour	359:364	arg1	TSG-6					401:405	TSG-6	401:405	TSG-6	401:405	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	46	theme	tumour	359:364	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	46	theme	tumour	359:364	arg1	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	0	47	theme	cumulus	52:58	arg1	signalling					65:74	cumulus cell signalling	52:74	cumulus cell signalling	52:74	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	7	48	theme	HA	1195:1196	arg1	levels					1205:1210	HA and HC levels	1195:1210	HA and HC levels	1195:1210	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	5	49	theme	DM	947:948	arg1	n=3					950:952	DM n=3	947:952	DM n=3 per group	947:962	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	6	50	theme	HCs	1005:1007	arg1	levels					991:996	the levels	987:996	the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group)	987:1083	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	1	51	theme	complex	129:135	arg1	expansion					143:151	Cumulus-oocyte complex (COC) expansion	114:151	Cumulus-oocyte complex (COC) expansion	114:151	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	10	52	theme	bone	1578:1581	arg1	synthesis					1608:1616	bone morphogenetic protein 15 synthesis	1578:1616	bone morphogenetic protein 15 synthesis	1578:1616	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	1	53	attach	linked	205:210	arg1	quality					222:228	oocyte quality	215:228	oocyte quality	215:228	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	1	53	attach	linked	205:210	arg2	expansion					143:151	Cumulus-oocyte complex (COC) expansion	114:151	Cumulus-oocyte complex (COC) expansion	114:151	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	8	54	theme	molecules	1324:1332	arg1	levels					1307:1312	the levels	1303:1312	the levels of matrix molecules	1303:1332	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	8	54	theme	molecules	1324:1332	arg1	area					1346:1349	cumulus area	1338:1349	cumulus area	1338:1349	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	3	55	theme	cumulus	440:446	arg1	cells					448:452	cumulus cells	440:452	cumulus cells	440:452	All except HCs are secreted by cumulus cells in response to oocyte-secreted factors, which signal via SMAD pathways.					
30373703	3	56	theme	SMAD	511:514	arg1	pathways					516:523	SMAD pathways	511:523	SMAD pathways	511:523	All except HCs are secreted by cumulus cells in response to oocyte-secreted factors, which signal via SMAD pathways.					
30373703	7	57	theme	HC	1202:1203	arg1	levels					1205:1210	HA and HC levels	1195:1210	HA and HC levels	1195:1210	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	5	58	dep	expansion	791:799	arg1	PMSG					847:850	PMSG	847:850	PMSG	847:850	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	5	58	dep	expansion	791:799	arg1	gonadotrophin					832:844	48 h-post-pregnant mare serum gonadotrophin	802:844	48 h-post-pregnant mare serum gonadotrophin (PMSG)	802:851	We compared COCs before expansion (48 h-post-pregnant mare serum gonadotrophin (PMSG)) and at late-stage expansion (9 h-post-human chorionic gonadotrophin (hCG); control n=3 mice, DM n=3 per group).					
30373703	9	59	theme	minimum	1479:1485	arg1	levels					1497:1502	minimum threshold levels	1479:1502	minimum threshold levels	1479:1502	These data suggest that HA and HCs can support cumulus expansion provided that they are present above minimum threshold levels.					
30373703	6	60	theme	PTX3	1010:1013	arg1	levels					991:996	the levels	987:996	the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group)	987:1083	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	6	61	theme	phosphorylated-SMAD1/5/8	1026:1049	arg1	levels					991:996	the levels	987:996	the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group)	987:1083	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	4	62	theme	DM	545:546	arg1	mouse					549:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	10	63	theme	protein	1597:1603	arg1	synthesis					1608:1616	bone morphogenetic protein 15 synthesis	1578:1616	bone morphogenetic protein 15 synthesis	1578:1616	We propose that oocyte-specific ablation of C1galt1 and Mgat1 may affect bone morphogenetic protein 15 synthesis or bioactivity, thereby reducing SMAD1/5/8 phosphorylation and HA production.					
30373703	6	64	theme	per	1074:1076	arg1	group					1078:1082	12-25 COCs per group	1063:1082	12-25 COCs per group	1063:1082	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	6	64	theme	per	1074:1076	arg1	phosphorylated-SMAD1/5/8					1026:1049	phosphorylated-SMAD1/5/8	1026:1049	phosphorylated-SMAD1/5/8	1026:1049	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	7	65	from	h-post-hCG	1174:1183	arg1	size					1148:1151	cumulus size	1140:1151	cumulus size	1140:1151	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	7	65	from	h-post-hCG	1174:1183	arg1	density					1161:1167	cell density	1156:1167	cell density	1156:1167	DM COCs did not differ from Controls in cumulus size or cell density at 9 h-post-hCG; however, HA and HC levels and phosphorylated-SMAD1/5/8 were reduced.					
30373703	2	66	theme	heavy	316:320	arg1	HCs					330:332	HCs	330:332	HCs	330:332	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	66	theme	heavy	316:320	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	66	theme	heavy	316:320	arg1	gene					393:396	tumour necrosis factor-stimulated gene 6	359:398	tumour necrosis factor-stimulated gene 6 (TSG-6)	359:406	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	66	theme	heavy	316:320	arg1	PTX3					349:352	pentraxin 3 (PTX3)	336:353	pentraxin 3 (PTX3)	336:353	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	8	67	located	found	1289:1293	arg2	correlations					1271:1282	no correlations	1268:1282	no correlations	1268:1282	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	8	67	located	found	1289:1293	arg1	samples					1368:1374	DM or Control samples	1354:1374	DM or Control samples	1354:1374	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	6	68	dep	assessed	1090:1097	arg1	Using					966:970	Using	966:970	Using histochemistry	966:985	Using histochemistry the levels of HA, HCs, PTX3, TSG-6 and phosphorylated-SMAD1/5/8 and -SMAD2 (12-25 COCs per group) were assessed.					
30373703	8	69	theme	matrix	1317:1322	arg1	molecules					1324:1332	matrix molecules	1317:1332	matrix molecules	1317:1332	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	1	70	theme	oocyte	215:220	arg1	quality					222:228	oocyte quality	215:228	oocyte quality	215:228	Cumulus-oocyte complex (COC) expansion is essential for ovulation and fertilisation and is linked to oocyte quality.					
30373703	4	71	theme	cumulus	748:754	arg1	expansion					756:764	modified cumulus expansion	739:764	modified cumulus expansion	739:764	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	4	72	theme	oocyte-specific	613:627	arg1	deletion					629:636	oocyte-specific deletion	613:636	oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1)	613:729	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	9	73	theme	cumulus	1424:1430	arg1	expansion					1432:1440	cumulus expansion	1424:1440	cumulus expansion	1424:1440	These data suggest that HA and HCs can support cumulus expansion provided that they are present above minimum threshold levels.					
30373703	0	74	theme	cell	60:63	arg1	signalling					65:74	cumulus cell signalling	52:74	cumulus cell signalling	52:74	Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition.					
30373703	8	75	theme	DM	1354:1355	arg1	samples					1368:1374	DM or Control samples	1354:1374	DM or Control samples	1354:1374	Furthermore, no correlations were found between the levels of matrix molecules and cumulus area in DM or Control samples.					
30373703	4	76	theme	modified	739:746	arg1	expansion					756:764	modified cumulus expansion	739:764	modified cumulus expansion	739:764	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	2	77	theme	pentraxin	336:344	arg1	PTX3					349:352	pentraxin 3 (PTX3)	336:353	pentraxin 3 (PTX3)	336:353	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	2	77	theme	pentraxin	336:344	arg1	chains					322:327	inter-α-inhibitor heavy chains	298:327	inter-α-inhibitor heavy chains (HCs)	298:333	Hyaluronan (HA), the major matrix constituent, is cross-linked via inter-α-inhibitor heavy chains (HCs), pentraxin 3 (PTX3) and tumour necrosis factor-stimulated gene 6 (TSG-6).					
30373703	4	78	contain	has	735:737	arg2	expansion					756:764	modified cumulus expansion	739:764	modified cumulus expansion	739:764	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
30373703	4	78	contain	has	735:737	arg1	mouse					549:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse	526:553	The double mutant (DM) mouse generates oocytes lacking complex N- and O-glycans due to oocyte-specific deletion of core 1 β1,3-galactosyltransferase (C1galt1) and N-acetylglucosaminyltransferase I (Mgat1) and has modified cumulus expansion.					
29580425	5	0	theme	base	602:605	arg1	matrices					607:614	base matrices	602:614	base matrices	602:614	Nano-Cellulose is synthesized via acid hydrolysis and incorporated in base matrices through wet processing.					
29580425	8	1	theme	nano-cellulose	895:908	arg1	composition					910:920	nano-cellulose composition	895:920	nano-cellulose composition	895:920	The results show that increasing nano-cellulose composition to 10% leads to increase the tensile strength at break to 8121 MN/m2 and decrease the elongation at break.					
29580425	9	2	theme	food	1094:1097	arg1	preservation					1099:1110	food preservation	1094:1110	food preservation up to 15 days	1094:1124	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	1	3	dep	Environmental	142:154	arg1	concerns					156:163	concerns	156:163	concerns	156:163	Environmental concerns have led to extensive research for replacing polymer-based food packaging with bio-nano-composites.					
29580425	6	4	theme	nano-composite	764:777	arg1	films					779:783	the nano-composite films	760:783	the nano-composite films	760:783	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	0	5	theme	food	113:116	arg1	applications					128:139	food packaging applications	113:139	food packaging applications	113:139	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	9	6	dep	%	1073:1073	arg1	to					1075:1076	to	1075:1076	to	1075:1076	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	9	7	from	%	1080:1080	arg1	composition					1055:1065	increasing chitosan composition	1035:1065	increasing chitosan composition from 5% to 30%	1035:1080	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	1	8	theme	extensive	177:185	arg1	research					187:194	extensive research	177:194	extensive research for replacing polymer-based food packaging with bio-nano-composites	177:262	Environmental concerns have led to extensive research for replacing polymer-based food packaging with bio-nano-composites.					
29580425	0	9	theme	starch-based	35:46	arg1	films					63:67	gelatin and starch-based nano-composite films	23:67	gelatin and starch-based nano-composite films	23:67	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	9	10	theme	increasing	1035:1044	arg1	composition					1055:1065	increasing chitosan composition	1035:1065	increasing chitosan composition from 5% to 30%	1035:1080	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	2	11	theme	nano-cellulose	297:310	arg1	incorporation					280:292	incorporation	280:292	incorporation of nano-cellulose into gelatin and starch matrices	280:343	In this study, incorporation of nano-cellulose into gelatin and starch matrices is investigated for this purpose.					
29580425	5	12	theme	acid	566:569	arg1	hydrolysis					571:580	acid hydrolysis	566:580	acid hydrolysis	566:580	Nano-Cellulose is synthesized via acid hydrolysis and incorporated in base matrices through wet processing.					
29580425	0	13	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study of gelatin and starch-based nano-composite films	0:67	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	3	14	theme	anti-fungal	419:429	arg1	properties					446:455	mechanical, anti-fungal and waterproof properties	407:455	mechanical, anti-fungal and waterproof properties	407:455	Chitosan is used to improve mechanical, anti-fungal and waterproof properties.					
29580425	3	15	used	used	391:394	arg2	Chitosan					379:386	Chitosan	379:386	Chitosan	379:386	Chitosan is used to improve mechanical, anti-fungal and waterproof properties.					
29580425	8	16	theme	tensile	951:957	arg1	strength					959:966	the tensile strength	947:966	the tensile strength at break to 8121 MN/m2	947:989	The results show that increasing nano-cellulose composition to 10% leads to increase the tensile strength at break to 8121 MN/m2 and decrease the elongation at break.					
29580425	8	17	from	break	971:975	arg1	strength					959:966	the tensile strength	947:966	the tensile strength at break to 8121 MN/m2	947:989	The results show that increasing nano-cellulose composition to 10% leads to increase the tensile strength at break to 8121 MN/m2 and decrease the elongation at break.					
29580425	1	18	theme	polymer-based	210:222	arg1	packaging					229:237	polymer-based food packaging	210:237	polymer-based food packaging	210:237	Environmental concerns have led to extensive research for replacing polymer-based food packaging with bio-nano-composites.					
29580425	3	19	theme	mechanical	407:416	arg1	properties					446:455	mechanical, anti-fungal and waterproof properties	407:455	mechanical, anti-fungal and waterproof properties	407:455	Chitosan is used to improve mechanical, anti-fungal and waterproof properties.					
29580425	7	20	theme	optimal	847:853	arg1	films					855:859	the optimal films	843:859	the optimal films	843:859	DSC/TGA and air permeability tests are also performed on the optimal films.					
29580425	0	21	theme	packaging	118:126	arg1	applications					128:139	food packaging applications	113:139	food packaging applications	113:139	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	2	22	theme	starch	329:334	arg1	matrices					336:343	starch matrices	329:343	starch matrices	329:343	In this study, incorporation of nano-cellulose into gelatin and starch matrices is investigated for this purpose.					
29580425	6	23	theme	contact	729:735	arg1	angle					737:741	visible and UV and water contact angle	704:741	angle	737:741	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	1	24	theme	food	224:227	arg1	packaging					229:237	polymer-based food packaging	210:237	polymer-based food packaging	210:237	Environmental concerns have led to extensive research for replacing polymer-based food packaging with bio-nano-composites.					
29580425	6	25	theme	tensile	646:652	arg1	strength					654:661	tensile strength	646:661	tensile strength test	646:666	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	26	theme	water	723:727	arg1	angle					737:741	visible and UV and water contact angle	704:741	angle	737:741	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	0	27	theme	gelatin	23:29	arg1	films					63:67	gelatin and starch-based nano-composite films	23:67	gelatin and starch-based nano-composite films	23:67	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	9	28	theme	chitosan	1046:1053	arg1	composition					1055:1065	increasing chitosan composition	1035:1065	increasing chitosan composition from 5% to 30%	1035:1080	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	4	29	theme	surface	511:517	arg1	methodology					519:529	response surface methodology	502:529	response surface methodology	502:529	Experiments are designed and analyzed using response surface methodology.					
29580425	0	30	theme	nano-composite	48:61	arg1	films					63:67	gelatin and starch-based nano-composite films	23:67	gelatin and starch-based nano-composite films	23:67	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	4	31	theme	response	502:509	arg1	methodology					519:529	response surface methodology	502:529	response surface methodology	502:529	Experiments are designed and analyzed using response surface methodology.					
29580425	3	32	theme	waterproof	435:444	arg1	properties					446:455	mechanical, anti-fungal and waterproof properties	407:455	mechanical, anti-fungal and waterproof properties	407:455	Chitosan is used to improve mechanical, anti-fungal and waterproof properties.					
29580425	5	33	theme	wet	624:626	arg1	processing					628:637	wet processing	624:637	wet processing	624:637	Nano-Cellulose is synthesized via acid hydrolysis and incorporated in base matrices through wet processing.					
29580425	9	34	theme	5	1072:1072	arg1	%					1073:1073	%	1073:1073	%	1073:1073	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	6	35	from	transparency	688:699	arg1	angle					737:741	visible and UV and water contact angle	704:741	angle	737:741	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	35	from	transparency	688:699	arg1	UV					716:717	visible and UV and water contact angle	704:741	UV	716:717	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	35	from	transparency	688:699	arg1	visible					704:710	visible	704:710	visible	704:710	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	8	36	from	break	1022:1026	arg1	elongation					1008:1017	the elongation	1004:1017	the elongation at break	1004:1026	The results show that increasing nano-cellulose composition to 10% leads to increase the tensile strength at break to 8121 MN/m2 and decrease the elongation at break.					
29580425	9	37	theme	30	1078:1079	arg1	%					1073:1073	%	1073:1073	%	1073:1073	Also, increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.					
29580425	7	38	theme	air	798:800	arg1	tests					815:819	air permeability tests	798:819	air permeability tests	798:819	DSC/TGA and air permeability tests are also performed on the optimal films.					
29580425	0	39	theme	films	63:67	arg1	study					14:18	A comparative study	0:18	A comparative study of gelatin and starch-based nano-composite films	0:67	A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications.					
29580425	6	40	theme	strength	654:661	arg1	transparency					688:699	transparency	688:699	transparency in visible and UV and water contact angle	688:741	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	40	theme	strength	654:661	arg1	test					663:666	tensile strength test	646:666	tensile strength test	646:666	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	40	theme	strength	654:661	arg1	preservation					674:685	food preservation	669:685	food preservation	669:685	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	41	theme	food	669:672	arg1	test					663:666	tensile strength test	646:666	tensile strength test	646:666	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	6	41	theme	food	669:672	arg1	preservation					674:685	food preservation	669:685	food preservation	669:685	Also, tensile strength test, food preservation, transparency in visible and UV and water contact angle are performed on the nano-composite films.					
29580425	7	42	theme	permeability	802:813	arg1	tests					815:819	air permeability tests	798:819	air permeability tests	798:819	DSC/TGA and air permeability tests are also performed on the optimal films.					
29463753	0	0	theme	gp120	81:85	arg1	antigenicity					65:76	antigenicity	65:76	antigenicity	65:76	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	0	0	theme	gp120	81:85	arg1	glycosylation					47:59	glycosylation	47:59	glycosylation	47:59	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	4	1	theme	specific	528:535	arg1	position					537:544	a specific position	526:544	a specific position in the Env signal peptide (SP)	526:575	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	1	2	theme	viruses	142:148	arg1	Env					116:118	Env	116:118	Env	116:118	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	2	theme	viruses	142:148	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	2	theme	viruses	142:148	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	12	3	theme	natural	1550:1556	arg1	variation					1558:1566	natural variation	1550:1566	natural variation in the SP of HIV Env	1550:1587	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	5	4	theme	gp120	655:659	arg1	glycosylation					661:673	gp120 glycosylation	655:673	gp120 glycosylation	655:673	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	12	5	theme	gp120	1641:1645	arg1	antigenicity					1618:1629	the antigenicity	1614:1629	the antigenicity of mature gp120	1614:1645	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	4	6	from	position	537:544	arg1	peptide					564:570	the Env signal peptide	549:570	the Env signal peptide (SP)	549:575	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	4	6	from	position	537:544	arg1	SP					573:574	SP	573:574	SP	573:574	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	3	7	theme	isolates	421:428	arg1	fitness					392:398	the fitness	388:398	the fitness of early-replicating isolates	388:428	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	4	8	from	signature	445:453	arg1	Env					458:460	Env	458:460	Env	458:460	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	12	9	theme	mature	1634:1639	arg1	gp120					1641:1645	mature gp120	1634:1645	mature gp120	1634:1645	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	8	10	theme	resonance	1063:1071	arg1	assay					1081:1085	a surface plasmon resonance binding assay	1045:1085	a surface plasmon resonance binding assay	1045:1085	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	4	11	theme	residues	514:521	arg1	overrepresentation					475:492	the overrepresentation	471:492	the overrepresentation of basic amino acid residues	471:521	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	5	12	theme	potential	614:622	arg1	impact					624:629	the potential impact	610:629	the potential impact of this SP signature on gp120 glycosylation and antigenicity	610:690	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	0	13	theme	Signal	0:5	arg1	peptide					7:13	Signal peptide	0:13	Signal peptide of HIV envelope protein	0:37	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	2	14	link	N-linked	258:265	arg1	PNGs					288:291	PNGs	288:291	PNGs	288:291	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	14	link	N-linked	258:265	arg1	sites					281:285	potential N-linked glycosylation sites	248:285	potential N-linked glycosylation sites (PNGs)	248:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	3	15	theme	posttranslational	353:369	arg1	modifications					371:383	posttranslational modifications	353:383	posttranslational modifications	353:383	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	4	16	theme	acid	509:512	arg1	residues					514:521	basic amino acid residues	497:521	basic amino acid residues	497:521	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	8	17	theme	plasmon	1055:1061	arg1	resonance					1063:1071	surface plasmon resonance	1047:1071	a surface plasmon resonance binding assay	1045:1085	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	4	18	theme	additional	434:443	arg1	signature					445:453	An additional signature	431:453	An additional signature in Env	431:460	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	6	19	from	isolate	827:833	arg1	second					794:799	second	794:799	second	794:799	Two recombinant gp120s were constructed, one derived from an isolate that lacks this signature and a second from an early-replicating isolate that includes this signature.					
29463753	1	20	theme	several	158:164	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	20	theme	several	158:164	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	3	21	theme	modifications	371:383	arg1	importance					339:348	the importance	335:348	the importance of posttranslational modifications in the fitness of early-replicating isolates	335:428	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	8	22	theme	surface	1047:1053	arg1	resonance					1063:1071	surface plasmon resonance	1047:1071	a surface plasmon resonance binding assay	1045:1085	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	8	23	theme	structure-	1001:1010	arg1	probes					1035:1040	glycan-, structure- and receptor- specific probes	992:1040	glycan-, structure- and receptor- specific probes	992:1040	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	2	24	theme	sites	281:285	arg1	number					238:243	a reduced number	228:243	a reduced number of potential N-linked glycosylation sites (PNGs)	228:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	1	25	theme	distinct	166:173	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	25	theme	distinct	166:173	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	3	26	theme	early-replicating	403:419	arg1	isolates					421:428	early-replicating isolates	403:428	early-replicating isolates	403:428	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	5	27	theme	SP	639:640	arg1	signature					642:650	this SP signature	634:650	this SP signature	634:650	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	7	28	theme	Chimeric	865:872	arg1	gp120s					874:879	Chimeric gp120s	865:879	Chimeric gp120s	865:879	Chimeric gp120s were also constructed in which the two SPs were swapped between the isolates.					
29463753	1	29	theme	transmission	175:186	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	29	theme	transmission	175:186	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	2	30	gly	glycosylation	267:279	arg2	sites					281:285	potential N-linked glycosylation sites	248:285	potential N-linked glycosylation sites (PNGs)	248:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	30	gly	glycosylation	267:279	arg2	PNGs					288:291	PNGs	288:291	PNGs	288:291	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	5	31	theme	signature	642:650	arg1	impact					624:629	the potential impact	610:629	the potential impact of this SP signature on gp120 glycosylation and antigenicity	610:690	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	8	32	theme	receptor-	1016:1024	arg1	probes					1035:1040	glycan-, structure- and receptor- specific probes	992:1040	glycan-, structure- and receptor- specific probes	992:1040	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	6	33	theme	recombinant	697:707	arg1	gp120s					709:714	Two recombinant gp120s	693:714	Two recombinant gp120s	693:714	Two recombinant gp120s were constructed, one derived from an isolate that lacks this signature and a second from an early-replicating isolate that includes this signature.					
29463753	8	34	theme	specific	1026:1033	arg1	probes					1035:1040	glycan-, structure- and receptor- specific probes	992:1040	glycan-, structure- and receptor- specific probes	992:1040	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	0	35	theme	HIV	18:20	arg1	protein					31:37	HIV envelope protein	18:37	HIV envelope protein	18:37	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	9	36	theme	Env	1208:1210	arg1	antigenicity					1192:1203	the antigenicity	1188:1203	the antigenicity of Env	1188:1210	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	1	37	theme	HIV-1	92:96	arg1	Env					116:118	Env	116:118	Env	116:118	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	37	theme	HIV-1	92:96	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	37	theme	HIV-1	92:96	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	6	38	attach	derived	738:744	arg2	one					734:736	one	734:736	one	734:736	Two recombinant gp120s were constructed, one derived from an isolate that lacks this signature and a second from an early-replicating isolate that includes this signature.					
29463753	6	38	attach	derived	738:744	arg1	isolate					754:760	an isolate	751:760	an isolate that lacks this signature	751:786	Two recombinant gp120s were constructed, one derived from an isolate that lacks this signature and a second from an early-replicating isolate that includes this signature.					
29463753	1	39	theme	envelope	98:105	arg1	Env					116:118	Env	116:118	Env	116:118	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	39	theme	envelope	98:105	arg1	protein					107:113	The HIV-1 envelope protein	88:113	The HIV-1 envelope protein (Env) of early-replicating viruses	88:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	1	39	theme	envelope	98:105	arg1	signatures					188:197	several distinct transmission signatures	158:197	several distinct transmission signatures	158:197	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	12	40	theme	Env	1585:1587	arg1	SP					1575:1576	the SP	1571:1576	the SP of HIV Env	1571:1587	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	10	41	theme	lectin	1278:1283	arg1	receptor					1285:1292	a lectin receptor	1276:1292	a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission	1276:1377	The SP impacts the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission.					
29463753	10	41	theme	lectin	1278:1283	arg1	DC-SIGN					1267:1273	DC-SIGN	1267:1273	DC-SIGN	1267:1273	The SP impacts the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission.					
29463753	0	42	theme	protein	31:37	arg1	peptide					7:13	Signal peptide	0:13	Signal peptide of HIV envelope protein	0:37	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	10	43	theme	Env	1259:1261	arg1	affinity					1247:1254	the affinity	1243:1254	the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission	1243:1377	The SP impacts the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission.					
29463753	12	44	from	variation	1558:1566	arg1	SP					1575:1576	the SP	1571:1576	the SP of HIV Env	1571:1587	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	9	45	theme	major	1217:1221	arg1	way					1223:1225	a major way	1215:1225	a major way	1215:1225	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	11	46	theme	monoclonal	1411:1420	arg1	antibodies					1422:1431	the monoclonal antibodies 17b and A32	1407:1443	the monoclonal antibodies 17b and A32	1407:1443	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	46	theme	monoclonal	1411:1420	arg1	A32					1441:1443	A32	1441:1443	A32	1441:1443	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	46	theme	monoclonal	1411:1420	arg1	17b					1433:1435	17b	1433:1435	17b	1433:1435	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	47	theme	Env	1498:1500	arg1	conformation					1482:1493	a CD4-induced, open conformation	1462:1493	a CD4-induced, open conformation of Env	1462:1500	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	0	48	theme	envelope	22:29	arg1	protein					31:37	HIV envelope protein	18:37	HIV envelope protein	18:37	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	4	49	theme	signal	557:562	arg1	peptide					564:570	the Env signal peptide	549:570	the Env signal peptide (SP)	549:575	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	4	49	theme	signal	557:562	arg1	SP					573:574	SP	573:574	SP	573:574	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	13	50	theme	antibody-mediated	1682:1698	arg1	pressure					1707:1714	antibody-mediated immune pressure	1682:1714	antibody-mediated immune pressure	1682:1714	Thus, the SP is likely subject to antibody-mediated immune pressure.					
29463753	4	51	theme	amino	503:507	arg1	residues					514:521	basic amino acid residues	497:521	basic amino acid residues	497:521	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	2	52	theme	reduced	230:236	arg1	number					238:243	a reduced number	228:243	a reduced number of potential N-linked glycosylation sites (PNGs)	228:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	9	53	theme	Env	1112:1114	arg1	SP					1106:1107	the SP	1102:1107	the SP of Env	1102:1114	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	4	54	theme	Env	553:555	arg1	peptide					564:570	the Env signal peptide	549:570	the Env signal peptide (SP)	549:575	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	4	54	theme	Env	553:555	arg1	SP					573:574	SP	573:574	SP	573:574	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	3	55	theme	transmission	300:311	arg1	signature					313:321	This transmission signature	295:321	This transmission signature	295:321	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	4	56	theme	basic	497:501	arg1	residues					514:521	basic amino acid residues	497:521	basic amino acid residues	497:521	An additional signature in Env involves the overrepresentation of basic amino acid residues at a specific position in the Env signal peptide (SP).					
29463753	8	57	theme	glycan-	992:998	arg1	probes					1035:1040	glycan-, structure- and receptor- specific probes	992:1040	glycan-, structure- and receptor- specific probes	992:1040	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	10	58	theme	mucosal	1358:1364	arg1	transmission					1366:1377	mucosal transmission	1358:1377	mucosal transmission	1358:1377	The SP impacts the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission.					
29463753	9	59	theme	qualitative	1127:1137	arg1	aspects					1139:1145	qualitative aspects	1127:1145	qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way	1127:1225	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	8	60	theme	binding	1073:1079	arg1	assay					1081:1085	a surface plasmon resonance binding assay	1045:1085	a surface plasmon resonance binding assay	1045:1085	All four gp120s were probed with glycan-, structure- and receptor- specific probes in a surface plasmon resonance binding assay.					
29463753	2	61	theme	such	204:207	arg1	signature					209:217	One such signature	200:217	One such signature	200:217	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	62	theme	glycosylation	267:279	arg1	PNGs					288:291	PNGs	288:291	PNGs	288:291	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	62	theme	glycosylation	267:279	arg1	sites					281:285	potential N-linked glycosylation sites	248:285	potential N-linked glycosylation sites (PNGs)	248:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	6	63	theme	early-replicating	809:825	arg1	isolate					827:833	an early-replicating isolate	806:833	an early-replicating isolate that includes this signature	806:862	Two recombinant gp120s were constructed, one derived from an isolate that lacks this signature and a second from an early-replicating isolate that includes this signature.					
29463753	11	64	dep	antibodies	1422:1431	arg1	antibodies					1422:1431	the monoclonal antibodies 17b and A32	1407:1443	the monoclonal antibodies 17b and A32	1407:1443	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	64	dep	antibodies	1422:1431	arg1	A32					1441:1443	A32	1441:1443	A32	1441:1443	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	64	dep	antibodies	1422:1431	arg1	17b					1433:1435	17b	1433:1435	17b	1433:1435	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	11	65	dep	CD4-induced	1464:1474	arg1	open					1477:1480	open	1477:1480	open	1477:1480	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	3	66	from	importance	339:348	arg1	fitness					392:398	the fitness	388:398	the fitness of early-replicating isolates	388:428	This transmission signature underscores the importance of posttranslational modifications in the fitness of early-replicating isolates.					
29463753	2	67	theme	N-linked	258:265	arg1	PNGs					288:291	PNGs	288:291	PNGs	288:291	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	67	theme	N-linked	258:265	arg1	sites					281:285	potential N-linked glycosylation sites	248:285	potential N-linked glycosylation sites (PNGs)	248:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	12	68	theme	HIV	1581:1583	arg1	Env					1585:1587	HIV Env	1581:1587	HIV Env	1581:1587	These results demonstrate that natural variation in the SP of HIV Env can significantly impact the antigenicity of mature gp120.					
29463753	5	69	from	impact	624:629	arg1	antigenicity					679:690	antigenicity	679:690	antigenicity	679:690	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	5	69	from	impact	624:629	arg1	glycosylation					661:673	gp120 glycosylation	655:673	gp120 glycosylation	655:673	In this report, we investigated the potential impact of this SP signature on gp120 glycosylation and antigenicity.					
29463753	10	70	theme	dendritic	1307:1315	arg1	cells					1317:1321	dendritic cells	1307:1321	dendritic cells that is believed to play a role in mucosal transmission	1307:1377	The SP impacts the affinity of Env for DC-SIGN, a lectin receptor expressed on dendritic cells that is believed to play a role in mucosal transmission.					
29463753	2	71	theme	potential	248:256	arg1	PNGs					288:291	PNGs	288:291	PNGs	288:291	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	2	71	theme	potential	248:256	arg1	sites					281:285	potential N-linked glycosylation sites	248:285	potential N-linked glycosylation sites (PNGs)	248:292	One such signature involves a reduced number of potential N-linked glycosylation sites (PNGs).					
29463753	9	72	theme	Env	1150:1152	arg1	glycosylation					1154:1166	Env glycosylation	1150:1166	Env glycosylation	1150:1166	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	0	73	gly	glycosylation	47:59	arg1	gp120					81:85	gp120	81:85	gp120	81:85	Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120.					
29463753	9	74	theme	glycosylation	1154:1166	arg1	aspects					1139:1145	qualitative aspects	1127:1145	qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way	1127:1225	We found that the SP of Env influences qualitative aspects of Env glycosylation that in turn affect the antigenicity of Env in a major way.					
29463753	1	75	theme	early-replicating	124:140	arg1	viruses					142:148	early-replicating viruses	124:148	early-replicating viruses	124:148	The HIV-1 envelope protein (Env) of early-replicating viruses encodes several distinct transmission signatures.					
29463753	11	76	theme	CD4-induced	1464:1474	arg1	conformation					1482:1493	a CD4-induced, open conformation	1462:1493	a CD4-induced, open conformation of Env	1462:1500	Additionally, affinity for the monoclonal antibodies 17b and A32, which recognize a CD4-induced, open conformation of Env is also altered.					
29463753	13	77	theme	immune	1700:1705	arg1	pressure					1707:1714	antibody-mediated immune pressure	1682:1714	antibody-mediated immune pressure	1682:1714	Thus, the SP is likely subject to antibody-mediated immune pressure.					
29636238	12	0	from	components	1984:1993	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	0	from	components	1984:1993	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	10	1	theme	direct	1680:1685	arg1	contact					1687:1693	direct contact	1680:1693	direct contact between the osteoid tissue and lamellar bone structures and beads	1680:1759	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	5	2	dep	In	816:817	arg1	vitro					819:823	vitro	819:823	vitro	819:823	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	12	3	theme	clinical	2229:2236	arg1	use					2238:2240	clinical use	2229:2240	clinical use	2229:2240	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	4	theme	dense	2076:2080	arg1	bone					2082:2085	a dense bone	2074:2085	a dense bone	2074:2085	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	3	5	theme	cell	570:573	arg1	adhesion					575:582	cell adhesion	570:582	cell adhesion	570:582	Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.					
29636238	4	6	from	dispersion	693:702	arg1	buffer					723:728	a physiological buffer	707:728	a physiological buffer	707:728	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	6	7	theme	scanning	1118:1125	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	7	theme	scanning	1118:1125	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	7	8	from	time	1238:1241	arg1	analyses					1209:1216	CBCT analyses	1204:1216	CBCT analyses at the implantation time	1204:1241	CBCT analyses at the implantation time revealed the radiolucent properties of these matrices.					
29636238	6	9	dep	microscopy	1136:1145	arg1	SEM					1148:1150	SEM	1148:1150	SEM	1148:1150	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	10	10	theme	bone	1735:1738	arg1	structures					1740:1749	the osteoid tissue and lamellar bone structures	1703:1749	the osteoid tissue and lamellar bone structures	1703:1749	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	1	11	theme	OBJECTIVE	139:147	arg1	matrices					180:187	OBJECTIVE Polysaccharide-based composite matrices	139:187	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA)	139:292	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	6	12	theme	months	1178:1183	arg1	post-implantation					1185:1201	6 months post-implantation	1176:1201	6 months post-implantation	1176:1201	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	9	13	theme	total	1452:1456	arg1	volume					1458:1463	total volume	1452:1463	total volume	1452:1463	The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss® used as positive control.					
29636238	4	14	theme	large	753:757	arg1	sheep					773:777	sheep	773:777	sheep	773:777	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	4	14	theme	large	753:757	arg1	model					766:770	a large animal model	751:770	a large animal model (sheep) for a sinus lift procedure	751:805	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	5	15	theme	Endothelial	938:948	arg1	EC					957:958	EC	957:958	EC	957:958	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	5	15	theme	Endothelial	938:948	arg1	Cells					950:954	Endothelial Cells	938:954	Endothelial Cells (EC)	938:959	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	2	16	theme	lift	461:464	arg1	model					466:470	a sinus lift model	453:470	a sinus lift model in the sheep	453:483	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	10	17	dep	tissue	1715:1720	arg1	the					1703:1705	the	1703:1705	the	1703:1705	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	0	18	theme	injectable	49:58	arg1	material					100:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	5	19	from	250-550μm	865:873	arg1	diameter					878:885	diameter	878:885	diameter	878:885	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	5	20	theme	vascular	894:901	arg1	adhesion					908:915	vascular cell adhesion	894:915	vascular cell adhesion	894:915	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	6	21	theme	In	992:993	arg1	studies					1000:1006	In vivo studies	992:1006	In vivo studies	992:1006	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	2	22	from	model	466:470	arg1	sheep					479:483	the sheep	475:483	the sheep	475:483	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	12	23	theme	osteoconductive	2013:2027	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	23	theme	osteoconductive	2013:2027	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	0	24	theme	composite	90:98	arg1	material					100:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	1	25	theme	natural	203:209	arg1	pullulan					228:235	pullulan	228:235	pullulan	228:235	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	1	25	theme	natural	203:209	arg1	polysaccharides					211:225	natural polysaccharides	203:225	natural polysaccharides	203:225	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	1	25	theme	natural	203:209	arg1	dextran					241:247	dextran	241:247	dextran	241:247	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	1	26	theme	composite	170:178	arg1	matrices					180:187	OBJECTIVE Polysaccharide-based composite matrices	139:187	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA)	139:292	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	6	27	dep	In	992:993	arg1	vivo					995:998	vivo	995:998	vivo	995:998	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	2	28	theme	sinus	455:459	arg1	model					466:470	a sinus lift model	453:470	a sinus lift model in the sheep	453:483	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	4	29	theme	sinus	786:790	arg1	procedure					797:805	a sinus lift procedure	784:805	a sinus lift procedure	784:805	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	0	30	theme	floor	119:123	arg1	augmentation					125:136	sinus floor augmentation	113:136	sinus floor augmentation	113:136	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	12	31	theme	material	2216:2223	arg1	great					2183:2187	great	2183:2187	great	2183:2187	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	11	32	from	network	1798:1804	arg1	contact					1905:1911	close contact	1899:1911	close contact	1899:1911	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	2	33	theme	new	431:433	arg1	formation					440:448	new bone formation	431:448	new bone formation	431:448	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	0	34	theme	In-vitro	0:7	arg1	design					21:26	design	21:26	design	21:26	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	4	35	from	METHODS	603:609	arg1	report					619:624	this report	614:624	this report	614:624	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	8	36	theme	dense	1344:1348	arg1	tissue					1362:1367	a dense mineralized tissue	1342:1367	a dense mineralized tissue	1342:1367	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	12	37	theme	initial	2120:2126	arg1	property					2140:2147	the initial radiolucent property	2116:2147	the initial radiolucent property to follow graft integration	2116:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	10	38	theme	inflammatory	1646:1657	arg1	events					1659:1664	any long term inflammatory events	1632:1664	any long term inflammatory events	1632:1664	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	0	39	theme	in-vivo	13:19	arg1	design					21:26	design	21:26	design	21:26	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	10	40	theme	tissue	1715:1720	arg1	structures					1740:1749	the osteoid tissue and lamellar bone structures	1703:1749	the osteoid tissue and lamellar bone structures	1703:1749	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	11	41	theme	vascularized	1869:1880	arg1	tissue					1889:1894	a newly vascularized formed tissue	1861:1894	a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials	1790:1931	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	8	42	theme	tissue	1362:1367	arg1	increase					1330:1337	an increase	1327:1337	an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation	1327:1421	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	10	43	theme	long	1636:1639	arg1	events					1659:1664	any long term inflammatory events	1632:1664	any long term inflammatory events	1632:1664	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	3	44	theme	in	524:525	arg1	properties					533:542	in vitro properties	524:542	in vitro properties of the material regarding cell adhesion and proliferation	524:600	Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.					
29636238	8	45	dep	months	1400:1405	arg1	up					1392:1393	up	1392:1393	up	1392:1393	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	6	46	theme	Beam	1087:1090	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	46	theme	Beam	1087:1090	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	5	47	dep	microbeads	853:862	arg1	250-550μm					865:873	250-550μm	865:873	250-550μm in diameter	865:885	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	11	48	theme	dense	1792:1796	arg1	network					1798:1804	a dense network	1790:1804	a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials	1790:1931	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	10	49	theme	osteoid	1707:1713	arg1	tissue					1715:1720	osteoid tissue	1707:1720	osteoid tissue	1707:1720	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	4	50	theme	such	632:635	arg1	matrix					647:652	such composite matrix	632:652	such composite matrix	632:652	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	2	51	theme	composite	401:409	arg1	material					411:418	this composite material	396:418	this composite material	396:418	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	12	52	theme	components	1984:1993	arg1	absence					1966:1972	the absence	1962:1972	the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue,	1962:2110	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	52	theme	components	1984:1993	arg1	SIGNIFICANCE					1934:1945	SIGNIFICANCE	1934:1945	SIGNIFICANCE In conclusion	1934:1959	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	52	theme	components	1984:1993	arg1	property					2140:2147	the initial radiolucent property	2116:2147	the initial radiolucent property to follow graft integration	2116:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	6	53	theme	formed	1052:1057	arg1	tissue					1059:1064	newly formed tissue	1046:1064	newly formed tissue	1046:1064	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	10	54	theme	lamellar	1726:1733	arg1	bone					1735:1738	lamellar bone	1726:1738	lamellar bone	1726:1738	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	8	55	from	increase	1330:1337	arg1	group					1386:1390	the Matrix-HA group	1372:1390	the Matrix-HA group	1372:1390	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	12	56	from	absence	1966:1972	arg1	conclusion					1950:1959	conclusion	1950:1959	conclusion	1950:1959	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	56	from	absence	1966:1972	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	56	from	absence	1966:1972	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	57	dep	great	2183:2187	arg1	promises					2189:2196	promises	2189:2196	promises	2189:2196	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	7	58	theme	implantation	1225:1236	arg1	time					1238:1241	the implantation time	1221:1241	the implantation time	1221:1241	CBCT analyses at the implantation time revealed the radiolucent properties of these matrices.					
29636238	2	59	theme	principal	328:336	arg1	objective					338:346	The principal objective	324:346	The principal objective of this study	324:360	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	5	60	dep	RESULTS	808:814	arg1	revealed					833:840	revealed	833:840	revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture	833:989	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	6	61	theme	electron	1127:1134	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	61	theme	electron	1127:1134	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	10	62	theme	Histological	1563:1574	arg1	examination					1576:1586	Histological examination	1563:1586	Histological examination	1563:1586	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	6	63	theme	CBCT	1113:1116	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	63	theme	CBCT	1113:1116	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	2	64	theme	study	356:360	arg1	objective					338:346	The principal objective	324:346	The principal objective of this study	324:360	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	12	65	dep	property	2140:2147	arg1	follow					2152:2157	follow	2152:2157	to follow graft integration	2149:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	9	66	theme	comparable	1488:1497	arg1	values					1499:1504	comparable values	1488:1504	comparable values	1488:1504	The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss® used as positive control.					
29636238	9	67	theme	mineralized	1428:1438	arg1	volume					1440:1445	The mineralized volume	1424:1445	The mineralized volume over total volume after 6 months	1424:1478	The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss® used as positive control.					
29636238	8	68	theme	implantation	1410:1421	arg1	months					1400:1405	3 months	1398:1405	3 months of implantation	1398:1421	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	4	69	theme	injectable	670:679	arg1	beads					681:685	injectable beads	670:685	injectable beads	670:685	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	4	69	theme	injectable	670:679	arg1	METHODS					603:609	METHODS	603:609	METHODS	603:609	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	11	70	theme	close	1899:1903	arg1	contact					1905:1911	close contact	1899:1911	close contact	1899:1911	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	12	71	from	Matrix-HA	2041:2049	arg1	sheep					2054:2058	sheep	2054:2058	sheep	2054:2058	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	11	72	from	tissue	1889:1894	arg1	contact					1905:1911	close contact	1899:1911	close contact	1899:1911	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	12	73	from	property	2029:2036	arg1	sheep					2054:2058	sheep	2054:2058	sheep	2054:2058	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	1	74	theme	Polysaccharide-based	149:168	arg1	matrices					180:187	OBJECTIVE Polysaccharide-based composite matrices	139:187	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA)	139:292	OBJECTIVE Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed.					
29636238	12	75	theme	vascularized	2091:2102	arg1	tissue					2104:2109	vascularized tissue	2091:2109	vascularized tissue	2091:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	76	theme	Matrix-HA	2041:2049	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	76	theme	Matrix-HA	2041:2049	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	0	77	theme	polysaccharide-hydroxyapatite	60:88	arg1	material					100:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	an injectable polysaccharide-hydroxyapatite composite material	46:107	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	4	78	theme	physiological	709:721	arg1	buffer					723:728	a physiological buffer	707:728	a physiological buffer	707:728	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	0	79	theme	material	100:107	arg1	validation					32:41	validation	32:41	validation	32:41	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	0	79	theme	material	100:107	arg1	design					21:26	design	21:26	design	21:26	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	12	80	from	Matrix-HA	1998:2006	arg1	absence					1966:1972	the absence	1962:1972	the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue,	1962:2110	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	80	from	Matrix-HA	1998:2006	arg1	SIGNIFICANCE					1934:1945	SIGNIFICANCE	1934:1945	SIGNIFICANCE In conclusion	1934:1959	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	80	from	Matrix-HA	1998:2006	arg1	property					2140:2147	the initial radiolucent property	2116:2147	the initial radiolucent property to follow graft integration	2116:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	5	81	theme	cell	903:906	arg1	adhesion					908:915	vascular cell adhesion	894:915	vascular cell adhesion	894:915	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	0	82	theme	sinus	113:117	arg1	augmentation					125:136	sinus floor augmentation	113:136	sinus floor augmentation	113:136	In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation.					
29636238	4	83	theme	lift	792:795	arg1	procedure					797:805	a sinus lift procedure	784:805	a sinus lift procedure	784:805	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	12	84	theme	composite	2206:2214	arg1	material					2216:2223	this composite material	2201:2223	this composite material for clinical use	2201:2240	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	8	85	theme	Quantitative	1298:1309	arg1	analysis					1311:1318	Quantitative analysis	1298:1318	Quantitative analysis	1298:1318	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	3	86	dep	in	524:525	arg1	vitro					527:531	vitro	527:531	vitro	527:531	Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.					
29636238	7	87	theme	matrices	1288:1295	arg1	properties					1268:1277	the radiolucent properties	1252:1277	the radiolucent properties of these matrices	1252:1295	CBCT analyses at the implantation time revealed the radiolucent properties of these matrices.					
29636238	2	88	theme	bone	435:438	arg1	formation					440:448	new bone formation	431:448	new bone formation	431:448	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	5	89	theme	In	816:817	arg1	studies					825:831	In vitro studies	816:831	In vitro studies	816:831	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	5	90	theme	Cells	950:954	arg1	proliferation					921:933	proliferation	921:933	proliferation of Endothelial Cells (EC)	921:959	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	5	90	theme	Cells	950:954	arg1	adhesion					908:915	vascular cell adhesion	894:915	vascular cell adhesion	894:915	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	4	91	theme	animal	759:764	arg1	sheep					773:777	sheep	773:777	sheep	773:777	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	4	91	theme	animal	759:764	arg1	model					766:770	a large animal model	751:770	a large animal model (sheep) for a sinus lift procedure	751:805	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	12	92	from	sheep	2054:2058	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	92	from	sheep	2054:2058	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	6	93	theme	adult	1029:1033	arg1	sheep					1035:1039	12 adult sheep	1026:1039	12 adult sheep	1026:1039	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	12	94	theme	graft	2159:2163	arg1	integration					2165:2175	graft integration	2159:2175	graft integration	2159:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	10	95	theme	term	1641:1644	arg1	events					1659:1664	any long term inflammatory events	1632:1664	any long term inflammatory events	1632:1664	Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads.					
29636238	12	96	from	SIGNIFICANCE	1934:1945	arg1	conclusion					1950:1959	conclusion	1950:1959	conclusion	1950:1959	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	96	from	SIGNIFICANCE	1934:1945	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	96	from	SIGNIFICANCE	1934:1945	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	9	97	theme	positive	1545:1552	arg1	control					1554:1560	positive control	1545:1560	positive control	1545:1560	The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss® used as positive control.					
29636238	8	98	theme	mineralized	1350:1360	arg1	tissue					1362:1367	a dense mineralized tissue	1342:1367	a dense mineralized tissue	1342:1367	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	3	99	theme	Secondary	486:494	arg1	objectives					496:505	Secondary objectives	486:505	Secondary objectives	486:505	Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.					
29636238	6	100	theme	Computed	1092:1099	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	100	theme	Computed	1092:1099	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	12	101	theme	radiolucent	2128:2138	arg1	property					2140:2147	the initial radiolucent property	2116:2147	the initial radiolucent property to follow graft integration	2116:2175	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	11	102	theme	osteocytes	1809:1818	arg1	tissue					1889:1894	a newly vascularized formed tissue	1861:1894	a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials	1790:1931	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	11	102	theme	osteocytes	1809:1818	arg1	network					1798:1804	a dense network	1790:1804	a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials	1790:1931	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	12	103	from	property	2140:2147	arg1	conclusion					1950:1959	conclusion	1950:1959	conclusion	1950:1959	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	103	from	property	2140:2147	arg1	Matrix-HA					1998:2006	Matrix-HA	1998:2006	Matrix-HA	1998:2006	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	12	103	from	property	2140:2147	arg1	property					2029:2036	the osteoconductive property	2009:2036	the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue	2009:2109	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	11	104	dep	vascularized	1869:1880	arg1	formed					1882:1887	formed	1882:1887	formed	1882:1887	After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.					
29636238	6	105	theme	Cone	1082:1085	arg1	microscopy					1136:1145	CBCT scanning electron microscopy	1113:1145	CBCT scanning electron microscopy	1113:1145	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	6	105	theme	Cone	1082:1085	arg1	Tomography					1101:1110	Cone Beam Computed Tomography	1082:1110	Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM)	1082:1151	In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation.					
29636238	4	106	theme	composite	637:645	arg1	matrix					647:652	such composite matrix	632:652	such composite matrix	632:652	METHODS In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.					
29636238	8	107	theme	Matrix-HA	1376:1384	arg1	group					1386:1390	the Matrix-HA group	1372:1390	the Matrix-HA group	1372:1390	Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation.					
29636238	2	108	theme	material	411:418	arg1	capacities					382:391	the capacities	378:391	the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep	378:483	The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep.					
29636238	12	109	theme	animal	1977:1982	arg1	components					1984:1993	animal components	1977:1993	animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue,	1977:2110	SIGNIFICANCE In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.					
29636238	5	110	theme	culture	983:989	arg1	days					975:978	1 and 7 days	967:978	1 and 7 days of culture	967:989	RESULTS In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture.					
29636238	7	111	theme	CBCT	1204:1207	arg1	analyses					1209:1216	CBCT analyses	1204:1216	CBCT analyses at the implantation time	1204:1241	CBCT analyses at the implantation time revealed the radiolucent properties of these matrices.					
29636238	7	112	theme	radiolucent	1256:1266	arg1	properties					1268:1277	the radiolucent properties	1252:1277	the radiolucent properties of these matrices	1252:1295	CBCT analyses at the implantation time revealed the radiolucent properties of these matrices.					
29636238	3	113	theme	material	551:558	arg1	properties					533:542	in vitro properties	524:542	in vitro properties of the material regarding cell adhesion and proliferation	524:600	Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.					
31905645	2	0	theme	fossil	351:356	arg1	WPC					397:399	WPC	397:399	WPC	397:399	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	0	theme	fossil	351:356	arg1	composites					385:394	conventional fossil polymer-based wood-plastic composites	338:394	conventional fossil polymer-based wood-plastic composites (WPC)	338:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	1	1	theme	composites	241:250	arg1	manufacturing					134:146	manufacturing	134:146	manufacturing	134:146	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	1	1	theme	composites	241:250	arg1	characterization					152:167	characterization	152:167	characterization	152:167	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	13	2	theme	MCC	1802:1804	arg1	modification					1815:1826	MCC chemical modification	1802:1826	MCC chemical modification	1802:1826	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	9	3	theme	glass	1301:1305	arg1	transition					1307:1316	The glass transition	1297:1316	The glass transition temperature	1297:1328	The glass transition temperature was directly affected by the composition and its value was -15 °C for PBS, -30 °C for EST, and -10 °C for MAH composites.					
31905645	4	4	theme	alkyl	629:633	arg1	EST					642:644	EST	642:644	EST	642:644	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	4	theme	alkyl	629:633	arg1	ester					635:639	alkyl ester	629:639	alkyl ester (EST)	629:645	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	2	5	theme	sustainable	290:300	arg1	composites					259:268	These composites	253:268	These composites	253:268	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	5	theme	sustainable	290:300	arg1	alternative					323:333	a sustainable woody-like composite alternative	288:333	a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC)	288:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	5	6	theme	filler	786:791	arg1	addition					774:781	The addition	770:781	The addition of filler into PBS	770:800	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	4	7	theme	polyhydroxy	603:613	arg1	amides					615:620	polyhydroxy amides	603:620	polyhydroxy amides (PHA)	603:626	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	7	theme	polyhydroxy	603:613	arg1	PHA					623:625	PHA	623:625	PHA	623:625	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	3	8	theme	MCC	478:480	arg1	MCC					478:480	MCC	478:480	MCC processed from bleached softwood	478:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	3	8	theme	MCC	478:480	arg1	%					473:473	70 wt%	468:473	70 wt% of MCC processed from bleached softwood	468:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	11	9	from	agglomeration	1615:1627	arg1	composites					1655:1664	the PBS/MCC composites	1643:1664	the PBS/MCC composites	1643:1664	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	1	10	theme	micro	208:212	arg1	cellulose					214:222	micro cellulose	208:222	micro cellulose	208:222	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	12	11	theme	polymer	1779:1785	arg1	crystallinity					1787:1799	polymer crystallinity	1779:1799	polymer crystallinity	1779:1799	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	11	12	theme	PBS/MCC	1647:1653	arg1	composites					1655:1664	the PBS/MCC composites	1643:1664	the PBS/MCC composites	1643:1664	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	5	13	theme	4.5-fold	811:818	arg1	improvement					820:830	a 4.5-fold improvement	809:830	a 4.5-fold improvement of Young's modulus E for the MCC composite	809:873	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	11	14	theme	microscopy	1581:1590	arg1	analysis					1592:1599	Scanning electron microscopy analysis	1563:1599	Scanning electron microscopy analysis	1563:1599	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	11	15	theme	Scanning	1563:1570	arg1	microscopy					1581:1590	Scanning electron microscopy	1563:1590	Scanning electron microscopy analysis	1563:1599	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	8	16	theme	viscoelasticity	1179:1193	arg1	properties					1195:1204	viscoelasticity properties	1179:1204	viscoelasticity properties	1179:1204	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	0	17	theme	Woody-Like	77:86	arg1	Application					107:117	Woody-Like Advanced Materials Application	77:117	Woody-Like Advanced Materials Application	77:117	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	1	18	dep	manufacturing	134:146	arg1	the					130:132	the	130:132	the	130:132	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	0	19	theme	Materials	97:105	arg1	Application					107:117	Woody-Like Advanced Materials Application	77:117	Woody-Like Advanced Materials Application	77:117	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	12	20	theme	particles	1751:1759	arg1	dispersion					1733:1742	the uniform dispersion	1721:1742	the uniform dispersion of MCC particles	1721:1759	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	12	20	theme	particles	1751:1759	arg1	decrease					1767:1774	a decrease	1765:1774	a decrease of polymer crystallinity	1765:1799	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	4	21	theme	anhydride	702:710	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	13	22	theme	composites	1884:1893	arg1	stability					1867:1875	the thermal stability	1855:1875	the thermal stability of MCC composites	1855:1893	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	2	23	theme	composite	313:321	arg1	composites					259:268	These composites	253:268	These composites	253:268	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	23	theme	composite	313:321	arg1	alternative					323:333	a sustainable woody-like composite alternative	288:333	a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC)	288:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	7	24	theme	storage	1044:1050	arg1	modulus					1052:1058	the storage modulus E'	1040:1061	the storage modulus E'	1040:1061	At room temperature, the storage modulus E' was found to improve by almost 4-fold for the APTMS composite.					
31905645	12	25	theme	uniform	1725:1731	arg1	dispersion					1733:1742	the uniform dispersion	1721:1742	the uniform dispersion of MCC particles	1721:1759	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	8	26	from	enhancement	1164:1174	arg1	properties					1195:1204	viscoelasticity properties	1179:1204	viscoelasticity properties	1179:1204	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	8	27	theme	long	1242:1245	arg1	chains					1253:1258	flexible long alkyl chains	1233:1258	flexible long alkyl chains	1233:1258	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	4	28	theme	polymeric	723:731	arg1	PMDI					763:766	PMDI	763:766	PMDI	763:766	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	28	theme	polymeric	723:731	arg1	diisocyanate					749:760	polymeric diphenylmethane diisocyanate	723:760	polymeric diphenylmethane diisocyanate (PMDI)	723:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	0	29	theme	Cellulose/Poly	14:27	arg1	Sustainable					50:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	12	30	theme	CDI	1684:1686	arg1	composites					1688:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	3	31	theme	melt	451:454	arg1	blending					456:463	a melt blending	449:463	a melt blending of 70 wt% of MCC processed from bleached softwood	449:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	8	32	theme	chains	1253:1258	arg1	introduction					1217:1228	the introduction	1213:1228	the introduction of flexible long alkyl chains	1213:1258	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	10	33	theme	bonding	1492:1498	arg1	generation					1471:1480	the generation	1467:1480	the generation of strong bonding between the polymer and cellulose components in the composite	1467:1560	FTIR indicated the generation of strong bonding between the polymer and cellulose components in the composite.					
31905645	0	34	theme	butylene	30:37	arg1	Sustainable					50:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	7	35	theme	room	1022:1025	arg1	temperature					1027:1037	room temperature	1022:1037	room temperature	1022:1037	At room temperature, the storage modulus E' was found to improve by almost 4-fold for the APTMS composite.					
31905645	6	36	theme	1.6-fold	907:914	arg1	increase					916:923	The 1.6-fold increase	903:923	The 1.6-fold increase of E	903:928	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	4	37	theme	diisocyanate	749:760	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	2	38	theme	conventional	338:349	arg1	WPC					397:399	WPC	397:399	WPC	397:399	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	38	theme	conventional	338:349	arg1	composites					385:394	conventional fossil polymer-based wood-plastic composites	338:394	conventional fossil polymer-based wood-plastic composites (WPC)	338:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	12	39	theme	PMDI	1667:1670	arg1	composites					1688:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	6	40	theme	unmodified	993:1002	arg1	composite					1008:1016	the unmodified MCC composite	989:1016	the unmodified MCC composite	989:1016	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	8	41	theme	other	1277:1281	arg1	compositions					1283:1294	other compositions	1277:1294	other compositions	1277:1294	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	13	42	theme	stability	1867:1875	arg1	enhancement					1840:1850	the enhancement	1836:1850	the enhancement of the thermal stability of MCC composites	1836:1893	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	3	43	theme	wt	471:472	arg1	MCC					478:480	MCC	478:480	MCC processed from bleached softwood	478:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	3	43	theme	wt	471:472	arg1	%					473:473	70 wt%	468:473	70 wt% of MCC processed from bleached softwood	468:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	1	44	theme	cellulose	214:222	arg1	composites					241:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	4	45	theme	ester	635:639	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	46	theme	carbodiimide	583:594	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	3	47	theme	bleached	497:504	arg1	softwood					506:513	bleached softwood	497:513	bleached softwood	497:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	7	48	located	found	1067:1071	arg2	modulus					1052:1058	the storage modulus E'	1040:1061	the storage modulus E'	1040:1061	At room temperature, the storage modulus E' was found to improve by almost 4-fold for the APTMS composite.					
31905645	7	48	located	found	1067:1071	arg1	temperature					1027:1037	room temperature	1022:1037	room temperature	1022:1037	At room temperature, the storage modulus E' was found to improve by almost 4-fold for the APTMS composite.					
31905645	2	49	theme	polymer-based	358:370	arg1	WPC					397:399	WPC	397:399	WPC	397:399	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	49	theme	polymer-based	358:370	arg1	composites					385:394	conventional fossil polymer-based wood-plastic composites	338:394	conventional fossil polymer-based wood-plastic composites (WPC)	338:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	1	50	theme	woody-like	230:239	arg1	composites					241:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	6	51	theme	modified	951:958	arg1	composition					960:970	CDI modified composition	947:970	CDI modified composition in comparison to the unmodified MCC composite	947:1016	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	10	52	from	components	1534:1543	arg1	composite					1552:1560	the composite	1548:1560	the composite	1548:1560	FTIR indicated the generation of strong bonding between the polymer and cellulose components in the composite.					
31905645	13	53	theme	chemical	1806:1813	arg1	modification					1815:1826	MCC chemical modification	1802:1826	MCC chemical modification	1802:1826	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	1	54	theme	butylene	178:185	arg1	poly					172:175	poly	172:175	poly (butylene succinate) (PBS)	172:202	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	1	54	theme	butylene	178:185	arg1	succinate					187:195	butylene succinate	178:195	butylene succinate	178:195	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	2	55	theme	woody-like	302:311	arg1	composites					259:268	These composites	253:268	These composites	253:268	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	55	theme	woody-like	302:311	arg1	alternative					323:333	a sustainable woody-like composite alternative	288:333	a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC)	288:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	9	56	theme	transition	1307:1316	arg1	temperature					1318:1328	The glass transition temperature	1297:1328	The glass transition temperature	1297:1328	The glass transition temperature was directly affected by the composition and its value was -15 °C for PBS, -30 °C for EST, and -10 °C for MAH composites.					
31905645	12	57	theme	crystallinity	1787:1799	arg1	dispersion					1733:1742	the uniform dispersion	1721:1742	the uniform dispersion of MCC particles	1721:1759	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	12	57	theme	crystallinity	1787:1799	arg1	decrease					1767:1774	a decrease	1765:1774	a decrease of polymer crystallinity	1765:1799	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	11	58	theme	MCC	1636:1638	arg1	agglomeration					1615:1627	the agglomeration	1611:1627	the agglomeration of the MCC in the PBS/MCC composites	1611:1664	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	4	59	theme	amides	615:620	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	8	60	theme	EST	1130:1132	arg1	composite					1134:1142	The EST composite	1126:1142	The EST composite	1126:1142	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	4	61	theme	maleic	690:695	arg1	MAH					713:715	MAH	713:715	MAH	713:715	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	61	theme	maleic	690:695	arg1	anhydride					702:710	maleic acid anhydride	690:710	maleic acid anhydride (MAH)	690:716	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	62	theme	3-Aminopropyl	649:661	arg1	APTMS					682:686	APTMS	682:686	APTMS	682:686	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	62	theme	3-Aminopropyl	649:661	arg1	trimethoxysilane					664:679	(3-Aminopropyl) trimethoxysilane	648:679	(3-Aminopropyl) trimethoxysilane (APTMS)	648:687	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	8	63	theme	pronounced	1153:1162	arg1	enhancement					1164:1174	a pronounced enhancement	1151:1174	a pronounced enhancement in viscoelasticity properties	1151:1204	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	11	64	theme	electron	1572:1579	arg1	microscopy					1581:1590	Scanning electron microscopy	1563:1590	Scanning electron microscopy analysis	1563:1599	Scanning electron microscopy analysis evidenced the agglomeration of the MCC in the PBS/MCC composites.					
31905645	0	65	theme	Advanced	88:95	arg1	Application					107:117	Woody-Like Advanced Materials Application	77:117	Woody-Like Advanced Materials Application	77:117	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	12	66	theme	MCC	1747:1749	arg1	particles					1751:1759	MCC particles	1747:1759	MCC particles	1747:1759	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	1	67	theme	poly	172:175	arg1	composites					241:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites	172:250	We report the manufacturing and characterization of poly (butylene succinate) (PBS) and micro cellulose (MCC) woody-like composites.					
31905645	10	68	theme	polymer	1512:1518	arg1	components					1534:1543	the polymer and cellulose components	1508:1543	components	1534:1543	FTIR indicated the generation of strong bonding between the polymer and cellulose components in the composite.					
31905645	13	69	theme	MCC	1880:1882	arg1	composites					1884:1893	MCC composites	1880:1893	MCC composites	1880:1893	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	5	70	theme	MCC	861:863	arg1	composite					865:873	the MCC composite	857:873	the MCC composite	857:873	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	6	71	theme	CDI	947:949	arg1	composition					960:970	CDI modified composition	947:970	CDI modified composition in comparison to the unmodified MCC composite	947:1016	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	4	72	theme	acid	697:700	arg1	MAH					713:715	MAH	713:715	MAH	713:715	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	72	theme	acid	697:700	arg1	anhydride					702:710	maleic acid anhydride	690:710	maleic acid anhydride (MAH)	690:716	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	0	73	theme	Loaded	7:12	arg1	Sustainable					50:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	5	74	theme	Young	835:839	arg1	E					851:851	Young's modulus E	835:851	Young's modulus E	835:851	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	4	75	theme	trimethoxysilane	664:679	arg1	way					576:578	way	576:578	way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI)	576:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	5	76	theme	modulus	843:849	arg1	E					851:851	Young's modulus E	835:851	Young's modulus E	835:851	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	6	77	theme	E	928:928	arg1	increase					916:923	The 1.6-fold increase	903:923	The 1.6-fold increase of E	903:928	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	8	78	theme	flexible	1233:1240	arg1	chains					1253:1258	flexible long alkyl chains	1233:1258	flexible long alkyl chains	1233:1258	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	0	79	theme	succinate	39:47	arg1	Sustainable					50:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable	0:60	Highly Loaded Cellulose/Poly (butylene succinate) Sustainable Composites for Woody-Like Advanced Materials Application.					
31905645	5	80	theme	neat	893:896	arg1	PBS					898:900	neat PBS	893:900	neat PBS	893:900	The addition of filler into PBS led to a 4.5-fold improvement of Young's modulus E for the MCC composite, in comparison to neat PBS.					
31905645	8	81	theme	alkyl	1247:1251	arg1	chains					1253:1258	flexible long alkyl chains	1233:1258	flexible long alkyl chains	1233:1258	The EST composite showed a pronounced enhancement in viscoelasticity properties due to the introduction of flexible long alkyl chains in comparison to other compositions.					
31905645	10	82	theme	strong	1485:1490	arg1	bonding					1492:1498	strong bonding	1485:1498	strong bonding between the polymer and cellulose components in the composite	1485:1560	FTIR indicated the generation of strong bonding between the polymer and cellulose components in the composite.					
31905645	12	83	theme	APTMS	1673:1677	arg1	composites					1688:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites	1667:1697	PMDI, APTMS, and CDI composites were characterized by the uniform dispersion of MCC particles and a decrease of polymer crystallinity.					
31905645	6	84	theme	MCC	1004:1006	arg1	composite					1008:1016	the unmodified MCC composite	989:1016	the unmodified MCC composite	989:1016	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	3	85	theme	PBS/MCC	407:413	arg1	composites					415:424	The PBS/MCC composites	403:424	The PBS/MCC composites	403:424	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	4	86	theme	diphenylmethane	733:747	arg1	PMDI					763:766	PMDI	763:766	PMDI	763:766	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	4	86	theme	diphenylmethane	733:747	arg1	diisocyanate					749:760	polymeric diphenylmethane diisocyanate	723:760	polymeric diphenylmethane diisocyanate (PMDI)	723:767	MCC was modified to enhance dispersion and compatibility by way of carbodiimide (CDI), polyhydroxy amides (PHA), alkyl ester (EST), (3-Aminopropyl) trimethoxysilane (APTMS), maleic acid anhydride (MAH), and polymeric diphenylmethane diisocyanate (PMDI).					
31905645	13	87	theme	thermal	1859:1865	arg1	stability					1867:1875	the thermal stability	1855:1875	the thermal stability of MCC composites	1855:1893	MCC chemical modification induced the enhancement of the thermal stability of MCC composites.					
31905645	10	88	theme	cellulose	1524:1532	arg1	components					1534:1543	the polymer and cellulose components	1508:1543	components	1534:1543	FTIR indicated the generation of strong bonding between the polymer and cellulose components in the composite.					
31905645	9	89	theme	MAH	1436:1438	arg1	composites					1440:1449	MAH composites	1436:1449	MAH composites	1436:1449	The glass transition temperature was directly affected by the composition and its value was -15 °C for PBS, -30 °C for EST, and -10 °C for MAH composites.					
31905645	7	90	theme	APTMS	1109:1113	arg1	composite					1115:1123	the APTMS composite	1105:1123	the APTMS composite	1105:1123	At room temperature, the storage modulus E' was found to improve by almost 4-fold for the APTMS composite.					
31905645	6	91	from	composition	960:970	arg1	comparison					975:984	comparison	975:984	comparison to the unmodified MCC composite	975:1016	The 1.6-fold increase of E was obtained for CDI modified composition in comparison to the unmodified MCC composite.					
31905645	3	92	theme	%	473:473	arg1	blending					456:463	a melt blending	449:463	a melt blending of 70 wt% of MCC processed from bleached softwood	449:513	The PBS/MCC composites were prepared by using a melt blending of 70 wt% of MCC processed from bleached softwood.					
31905645	2	93	theme	wood-plastic	372:383	arg1	WPC					397:399	WPC	397:399	WPC	397:399	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
31905645	2	93	theme	wood-plastic	372:383	arg1	composites					385:394	conventional fossil polymer-based wood-plastic composites	338:394	conventional fossil polymer-based wood-plastic composites (WPC)	338:400	These composites can be applied as a sustainable woody-like composite alternative to conventional fossil polymer-based wood-plastic composites (WPC).					
30691252	2	0	theme	MalQ	460:463	arg1	involvement					445:455	the involvement	441:455	the involvement of MalQ in glycogen degradation	441:487	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	4	1	theme	faster	899:904	arg1	glucose-1-P					870:880	glucose-1-P	870:880	glucose-1-P (glc-1-P) 3-fold faster than without MalQ	870:922	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	4	1	theme	faster	899:904	arg1	glc-1-P					883:889	glc-1-P	883:889	glc-1-P	883:889	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	3	2	theme	coli	686:689	arg1	GlgP					772:775	GlgP	772:775	GlgP	772:775	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	2	theme	coli	686:689	arg1	MalP					737:740	MalP	737:740	MalP (maltodextrin phosphorylase)	737:769	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	2	theme	coli	686:689	arg1	enzymes					691:697	E. coli enzymes	683:697	E. coli enzymes	683:697	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	2	theme	coli	686:689	arg1	GlgX					710:713	GlgX	710:713	GlgX (debranching enzyme)	710:734	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	2	theme	coli	686:689	arg1	MalQ					781:784	MalQ	781:784	MalQ	781:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	3	dep	cyclodextrin	551:562	arg1	maltotetraosyl-β-CD					565:583	maltotetraosyl-β-CD	565:583	maltotetraosyl-β-CD: G4- β-CD	565:593	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	8	4	theme	MalQ-involved	1511:1523	arg1	pathways					1525:1532	two possible MalQ-involved pathways	1498:1532	two possible MalQ-involved pathways in glycogen degradation	1498:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	7	5	theme	resultant	1351:1359	arg1	G4					1361:1362	the resultant G4	1347:1362	the resultant G4	1347:1362	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	1	6	dep	in	269:270	arg1	vivo					272:275	vivo	272:275	vivo	272:275	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	4	7	from	reaction	815:822	arg1	substrates					850:859	substrates	850:859	substrates	850:859	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	1	8	theme	in	256:257	arg1	assays					277:282	both in vitro and in vivo assays	251:282	both in vitro and in vivo assays	251:282	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	9	9	theme	operon	1563:1568	arg1	structure					1570:1578	The operon structure	1559:1578	The operon structure of MalP-defecting enterobacteria	1559:1611	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	3	10	theme	variable	658:665	arg1	combinations					667:678	variable combinations	658:678	variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ	658:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	6	11	theme	MalQ	1142:1145	arg1	reaction					1115:1122	the reaction	1111:1122	the reaction of GlgX, GlgP and MalQ	1111:1145	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	3	12	theme	glycogen-mimic	516:529	arg1	substrate					531:539	glycogen-mimic substrate	516:539	glycogen-mimic substrate	516:539	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	13	theme	branched	542:549	arg1	cyclodextrin					551:562	branched cyclodextrin	542:562	branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD)	542:594	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	0	14	theme	Role	93:96	arg1	Reaction					43:50	the Transglycosylation Reaction	20:50	the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli	20:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	3	15	theme	enzymes	691:697	arg1	combinations					667:678	variable combinations	658:678	variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ	658:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	7	16	theme	GlgX	1306:1309	arg1	hydrolysis					1311:1320	GlgX hydrolysis	1306:1320	GlgX hydrolysis	1306:1320	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	9	17	theme	enterobacteria	1598:1611	arg1	structure					1570:1578	The operon structure	1559:1578	The operon structure of MalP-defecting enterobacteria	1559:1611	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	0	18	from	4-α-Glucanotransferase	55:76	arg1	coli					135:138	Escherichia coli	123:138	Escherichia coli	123:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	0	18	from	4-α-Glucanotransferase	55:76	arg1	Breakdown					110:118	Glycogen Breakdown	101:118	Glycogen Breakdown	101:118	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	6	19	theme	GlgP	1133:1136	arg1	reaction					1115:1122	the reaction	1111:1122	the reaction of GlgX, GlgP and MalQ	1111:1145	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	9	20	theme	alternative	1667:1677	arg1	pathways					1679:1686	alternative pathways	1667:1686	alternative pathways in glycogen degradation	1667:1710	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	9	20	theme	alternative	1667:1677	arg1	involvement					1635:1645	the involvement	1631:1645	the involvement of MalQ and GlgP	1631:1662	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	3	21	dep	maltotetraosyl-β-CD	565:583	arg1	β-CD					590:593	G4- β-CD	586:593	maltotetraosyl-β-CD: G4- β-CD	565:593	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	0	22	theme	Glycogen	101:108	arg1	Breakdown					110:118	Glycogen Breakdown	101:118	Glycogen Breakdown	101:118	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	2	23	from	involvement	445:455	arg1	degradation					477:487	glycogen degradation	468:487	glycogen degradation	468:487	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	0	24	from	coli	135:138	arg1	Reaction					43:50	the Transglycosylation Reaction	20:50	the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli	20:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	0	25	from	Reaction	43:50	arg1	coli					135:138	Escherichia coli	123:138	Escherichia coli	123:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	0	25	from	Reaction	43:50	arg1	Breakdown					110:118	Glycogen Breakdown	101:118	Glycogen Breakdown	101:118	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	6	26	theme	GlgX	1127:1130	arg1	reaction					1115:1122	the reaction	1111:1122	the reaction of GlgX, GlgP and MalQ	1111:1145	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	7	27	theme	GlgP-limit	1271:1280	arg1	dextrin					1282:1288	GlgP-limit dextrin	1271:1288	GlgP-limit dextrin	1271:1288	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	5	28	attach	released	983:990	arg1	G4					1037:1038	G4	1037:1038	G4	1037:1038	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	5	28	attach	released	983:990	arg1	dextrin					1008:1014	GlgP-limit dextrin	997:1014	GlgP-limit dextrin to another acceptor	997:1034	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	5	28	attach	released	983:990	arg2	G4					980:981	disproportionate G4	963:981	disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP	963:1071	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	1	29	theme	MalQ	179:182	arg1	involvement					164:174	the involvement	160:174	the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown	160:246	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	2	30	theme	wild-type	412:420	arg1	strain					422:427	the wild-type strain	408:427	the wild-type strain	408:427	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	1	31	theme	in	269:270	arg1	assays					277:282	both in vitro and in vivo assays	251:282	both in vitro and in vivo assays	251:282	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	0	32	from	Role	93:96	arg1	coli					135:138	Escherichia coli	123:138	Escherichia coli	123:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	0	32	from	Role	93:96	arg1	Breakdown					110:118	Glycogen Breakdown	101:118	Glycogen Breakdown	101:118	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	7	33	theme	dextrin	1282:1288	arg1	chains					1261:1266	the G4-branch chains	1247:1266	the G4-branch chains of GlgP-limit dextrin	1247:1288	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	0	34	theme	Transglycosylation	24:41	arg1	Reaction					43:50	the Transglycosylation Reaction	20:50	the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli	20:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	3	35	theme	debranching	716:726	arg1	GlgX					710:713	GlgX	710:713	GlgX (debranching enzyme)	710:734	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	35	theme	debranching	716:726	arg1	enzyme					728:733	debranching enzyme	716:733	debranching enzyme	716:733	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	9	36	theme	MalQ	1650:1653	arg1	pathways					1679:1686	alternative pathways	1667:1686	alternative pathways in glycogen degradation	1667:1710	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	9	36	theme	MalQ	1650:1653	arg1	involvement					1635:1645	the involvement	1631:1645	the involvement of MalQ and GlgP	1631:1662	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	3	37	dep	In	490:491	arg1	vitro					493:497	vitro	493:497	vitro	493:497	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	4	38	theme	GlgX	833:836	arg1	reaction					815:822	the reaction	811:822	the reaction of MalP, GlgX and MalQ on substrates	811:859	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	2	39	theme	In	285:286	arg1	tests					293:297	In vivo tests	285:297	In vivo tests of the knock-out mutant, ΔmalQ,	285:329	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	2	40	dep	In	285:286	arg1	vivo					288:291	vivo	288:291	vivo	288:291	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	6	41	theme	glc-1-P	1194:1200	arg1	production					1180:1189	a 1.6-fold increased production	1159:1189	a 1.6-fold increased production of glc-1-P	1159:1200	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	3	42	theme	-limit	629:634	arg1	dextrin					636:642	-limit dextrin	629:642	-limit dextrin	629:642	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	43	theme	G4-	586:588	arg1	β-CD					590:593	G4- β-CD	586:593	maltotetraosyl-β-CD: G4- β-CD	565:593	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	2	44	theme	stationary	379:388	arg1	phase					390:394	the stationary phase	375:394	the stationary phase compared to the wild-type strain	375:427	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	0	45	theme	Reaction	43:50	arg1	Characterization					0:15	Characterization	0:15	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.	0:139	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	4	46	theme	MalQ	842:845	arg1	reaction					815:822	the reaction	811:822	the reaction of MalP, GlgX and MalQ on substrates	811:859	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	7	47	theme	branch	1382:1387	arg1	chain					1389:1393	another branch chain	1374:1393	another branch chain	1374:1393	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	7	48	theme	G4-branch	1251:1259	arg1	chains					1261:1266	the G4-branch chains	1247:1266	the G4-branch chains of GlgP-limit dextrin	1247:1288	The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP.					
30691252	1	49	theme	Escherichia	212:222	arg1	breakdown					238:246	Escherichia coli glycogen breakdown	212:246	Escherichia coli glycogen breakdown	212:246	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	4	50	dep	GlgP	805:808	arg1	the					790:792	the	790:792	the	790:792	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	4	50	dep	GlgP	805:808	arg1	absence					794:800	absence	794:800	absence	794:800	In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ.					
30691252	5	51	theme	GlgP-limit	997:1006	arg1	G4					1037:1038	G4	1037:1038	G4	1037:1038	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	5	51	theme	GlgP-limit	997:1006	arg1	dextrin					1008:1014	GlgP-limit dextrin	997:1014	GlgP-limit dextrin to another acceptor	997:1034	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	3	52	theme	E.	683:684	arg1	GlgP					772:775	GlgP	772:775	GlgP	772:775	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	52	theme	E.	683:684	arg1	MalP					737:740	MalP	737:740	MalP (maltodextrin phosphorylase)	737:769	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	52	theme	E.	683:684	arg1	enzymes					691:697	E. coli enzymes	683:697	E. coli enzymes	683:697	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	52	theme	E.	683:684	arg1	GlgX					710:713	GlgX	710:713	GlgX (debranching enzyme)	710:734	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	52	theme	E.	683:684	arg1	MalQ					781:784	MalQ	781:784	MalQ	781:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	0	53	theme	4-α-Glucanotransferase	55:76	arg1	Reaction					43:50	the Transglycosylation Reaction	20:50	the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli	20:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	6	54	theme	increased	1170:1178	arg1	production					1180:1189	a 1.6-fold increased production	1159:1189	a 1.6-fold increased production of glc-1-P	1159:1200	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	0	55	from	Breakdown	110:118	arg1	Reaction					43:50	the Transglycosylation Reaction	20:50	the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli	20:138	Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli.					
30691252	8	56	from	degradation	1546:1556	arg1	model					1489:1493	a model	1487:1493	a model of two possible MalQ-involved pathways in glycogen degradation	1487:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	9	57	theme	GlgP	1659:1662	arg1	pathways					1679:1686	alternative pathways	1667:1686	alternative pathways in glycogen degradation	1667:1710	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	9	57	theme	GlgP	1659:1662	arg1	involvement					1635:1645	the involvement	1631:1645	the involvement of MalQ and GlgP	1631:1662	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	2	58	theme	mutant	316:321	arg1	tests					293:297	In vivo tests	285:297	In vivo tests of the knock-out mutant, ΔmalQ,	285:329	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	5	59	theme	disproportionate	963:978	arg1	G4					980:981	disproportionate G4	963:981	disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP	963:1071	The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP.					
30691252	9	60	theme	MalP-defecting	1583:1596	arg1	enterobacteria					1598:1611	MalP-defecting enterobacteria	1583:1611	MalP-defecting enterobacteria	1583:1611	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	3	61	theme	glycogen	600:607	arg1	GlgP					624:627	GlgP	624:627	GlgP	624:627	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	61	theme	glycogen	600:607	arg1	phosphorylase					609:621	glycogen phosphorylase	600:621	glycogen phosphorylase (GlgP)	600:628	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	2	62	theme	knock-out	306:314	arg1	mutant					316:321	the knock-out mutant	302:321	the knock-out mutant	302:321	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	2	62	theme	knock-out	306:314	arg1	ΔmalQ					324:328	ΔmalQ	324:328	ΔmalQ	324:328	In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation.					
30691252	8	63	from	pathways	1525:1532	arg1	degradation					1546:1556	glycogen degradation	1537:1556	glycogen degradation	1537:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	8	64	from	model	1489:1493	arg1	degradation					1546:1556	glycogen degradation	1537:1556	glycogen degradation	1537:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	1	65	dep	Escherichia	212:222	arg1	coli					224:227	coli	224:227	coli	224:227	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	3	66	dep	substrate	531:539	arg1	dextrin					636:642	-limit dextrin	629:642	-limit dextrin	629:642	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	8	67	theme	possible	1502:1509	arg1	pathways					1525:1532	two possible MalQ-involved pathways	1498:1532	two possible MalQ-involved pathways in glycogen degradation	1498:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	1	68	theme	glycogen	229:236	arg1	breakdown					238:246	Escherichia coli glycogen breakdown	212:246	Escherichia coli glycogen breakdown	212:246	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	1	69	dep	in	256:257	arg1	vitro					259:263	vitro	259:263	vitro	259:263	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	3	70	theme	In	490:491	arg1	assays					499:504	In vitro assays	490:504	In vitro assays	490:504	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	9	71	from	pathways	1679:1686	arg1	degradation					1700:1710	glycogen degradation	1691:1710	glycogen degradation	1691:1710	The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.					
30691252	3	72	theme	combinations	667:678	arg1	combinations					667:678	variable combinations	658:678	variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ	658:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	72	theme	combinations	667:678	arg1	set					651:653	a set	649:653	a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ	649:784	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	1	73	from	involvement	164:174	arg1	breakdown					238:246	Escherichia coli glycogen breakdown	212:246	Escherichia coli glycogen breakdown	212:246	We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays.					
30691252	6	74	dep	MalP	1105:1108	arg1	absence					1094:1100	absence	1094:1100	absence	1094:1100	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	6	74	dep	MalP	1105:1108	arg1	the					1090:1092	the	1090:1092	the	1090:1092	In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ.					
30691252	8	75	theme	pathways	1525:1532	arg1	model					1489:1493	a model	1487:1493	a model of two possible MalQ-involved pathways in glycogen degradation	1487:1556	Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation.					
30691252	3	76	theme	maltodextrin	743:754	arg1	phosphorylase					756:768	maltodextrin phosphorylase	743:768	maltodextrin phosphorylase	743:768	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
30691252	3	76	theme	maltodextrin	743:754	arg1	MalP					737:740	MalP	737:740	MalP (maltodextrin phosphorylase)	737:769	In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4- β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ.					
29933001	1	0	theme	polysaccharides	203:217	arg1	analysis					191:198	composition analysis	179:198	composition analysis of polysaccharides	179:217	Releasing all monosaccharides during acid hydrolysis for composition analysis of polysaccharides has been a time consuming process.					
29933001	0	1	theme	polysaccharides	105:119	arg1	analyses					83:90	monosaccharide composition analyses	56:90	monosaccharide composition analyses of different polysaccharides	56:119	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	3	2	theme	neutral	759:765	arg1	polysaccharides					767:781	acidic, basic, and neutral polysaccharides	740:781	acidic, basic, and neutral polysaccharides	740:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	3	3	theme	chondroitin	894:904	arg1	sulfates					906:913	chondroitin sulfates	894:913	chondroitin sulfates	894:913	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	3	4	theme	hydrolysis	638:647	arg1	procedure					649:657	the conventional hydrolysis procedure	621:657	the conventional hydrolysis procedure	621:657	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	2	5	theme	sensitive	314:322	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	6	theme	detection	583:591	arg1	analysis					599:606	pulsed amperometric detection (PAD) analysis	563:606	pulsed amperometric detection (PAD) analysis	563:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	0	7	theme	microwave-assisted	11:28	arg1	conditions					41:50	microwave-assisted hydrolysis conditions	11:50	microwave-assisted hydrolysis conditions	11:50	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	2	8	theme	amperometric	570:581	arg1	PAD					594:596	PAD	594:596	PAD	594:596	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	8	theme	amperometric	570:581	arg1	detection					583:591	pulsed amperometric detection	563:591	pulsed amperometric detection (PAD) analysis	563:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	9	theme	composition	350:360	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	10	theme	high-performance	494:509	arg1	HPAEC					542:546	HPAEC	542:546	HPAEC	542:546	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	10	theme	high-performance	494:509	arg1	chromatography					526:539	high-performance anion-exchange chromatography	494:539	high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis	494:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	11	theme	monosaccharide	335:348	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	12	theme	HCl	435:437	arg1	10 min					451:456	10 min	451:456	10 min	451:456	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	12	theme	HCl	435:437	arg1	hydrolysis					439:448	microwave-assisted HCl hydrolysis	416:448	microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis	416:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	13	theme	quick	329:333	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	14	theme	polysaccharides	466:480	arg1	10 min					451:456	10 min	451:456	10 min	451:456	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	14	theme	polysaccharides	466:480	arg1	hydrolysis					439:448	microwave-assisted HCl hydrolysis	416:448	microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis	416:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	0	15	theme	hydrolysis	30:39	arg1	conditions					41:50	microwave-assisted hydrolysis conditions	11:50	microwave-assisted hydrolysis conditions	11:50	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	3	16	theme	conventional	625:636	arg1	procedure					649:657	the conventional hydrolysis procedure	621:657	the conventional hydrolysis procedure	621:657	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	0	17	theme	monosaccharide	56:69	arg1	analyses					83:90	monosaccharide composition analyses	56:90	monosaccharide composition analyses of different polysaccharides	56:119	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	2	18	theme	efficient	275:283	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	1	19	theme	consuming	235:243	arg1	process					245:251	a time consuming process	228:251	a time consuming process	228:251	Releasing all monosaccharides during acid hydrolysis for composition analysis of polysaccharides has been a time consuming process.					
29933001	2	20	dep	efficient	275:283	arg1	acid					307:310	10 μL sample + 10 μL acid	286:310	10 μL sample + 10 μL acid	286:310	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	3	21	theme	monosaccharide	701:714	arg1	analysis					728:735	monosaccharide composition analysis	701:735	monosaccharide composition analysis of acidic, basic, and neutral polysaccharides	701:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	3	22	theme	composition	716:726	arg1	analysis					728:735	monosaccharide composition analysis	701:735	monosaccharide composition analysis of acidic, basic, and neutral polysaccharides	701:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	1	23	theme	acid	159:162	arg1	hydrolysis					164:173	acid hydrolysis	159:173	acid hydrolysis for composition analysis of polysaccharides	159:217	Releasing all monosaccharides during acid hydrolysis for composition analysis of polysaccharides has been a time consuming process.					
29933001	0	24	theme	composition	71:81	arg1	analyses					83:90	monosaccharide composition analyses	56:90	monosaccharide composition analyses of different polysaccharides	56:119	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	2	25	theme	current	257:263	arg1	study					265:269	current study	257:269	current study	257:269	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	26	theme	polysaccharides	374:388	arg1	analysis					362:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis	272:369	an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides	272:388	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	3	27	theme	acidic	740:745	arg1	polysaccharides					767:781	acidic, basic, and neutral polysaccharides	740:781	acidic, basic, and neutral polysaccharides	740:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	2	28	theme	sample + 10 μL	292:305	arg1	acid					307:310	10 μL sample + 10 μL acid	286:310	10 μL sample + 10 μL acid	286:310	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	1	29	theme	composition	179:189	arg1	analysis					191:198	composition analysis	179:198	composition analysis of polysaccharides	179:217	Releasing all monosaccharides during acid hydrolysis for composition analysis of polysaccharides has been a time consuming process.					
29933001	0	30	theme	different	95:103	arg1	polysaccharides					105:119	different polysaccharides	95:119	different polysaccharides	95:119	Optimizing microwave-assisted hydrolysis conditions for monosaccharide composition analyses of different polysaccharides.					
29933001	2	31	theme	10 μL	286:290	arg1	acid					307:310	10 μL sample + 10 μL acid	286:310	10 μL sample + 10 μL acid	286:310	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	32	theme	microwave-assisted	416:433	arg1	10 min					451:456	10 min	451:456	10 min	451:456	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	32	theme	microwave-assisted	416:433	arg1	hydrolysis					439:448	microwave-assisted HCl hydrolysis	416:448	microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis	416:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	3	33	theme	basic	748:752	arg1	polysaccharides					767:781	acidic, basic, and neutral polysaccharides	740:781	acidic, basic, and neutral polysaccharides	740:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	2	34	theme	pulsed	563:568	arg1	PAD					594:596	PAD	594:596	PAD	594:596	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	34	theme	pulsed	563:568	arg1	detection					583:591	pulsed amperometric detection	563:591	pulsed amperometric detection (PAD) analysis	563:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	3	35	theme	polysaccharides	767:781	arg1	analysis					728:735	monosaccharide composition analysis	701:735	monosaccharide composition analysis of acidic, basic, and neutral polysaccharides	701:781	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	3	36	theme	efficient	678:686	arg1	method					665:670	this method	660:670	this method	660:670	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	3	36	theme	efficient	678:686	arg1	approach					688:695	an efficient approach	675:695	an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates	675:913	Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.					
29933001	2	37	theme	anion-exchange	511:524	arg1	HPAEC					542:546	HPAEC	542:546	HPAEC	542:546	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29933001	2	37	theme	anion-exchange	511:524	arg1	chromatography					526:539	high-performance anion-exchange chromatography	494:539	high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis	494:606	In current study, an efficient (10 μL sample + 10 μL acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10 min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis.					
29297680	1	0	contain	have	124:127	arg2	functions					173:181	biological functions	162:181	biological functions	162:181	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	1	0	contain	have	124:127	arg1	glycoconjugates					108:122	glycoconjugates	108:122	glycoconjugates	108:122	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	1	0	contain	have	124:127	arg1	glycans					96:102	Naturally occurring glycans	76:102	Naturally occurring glycans	76:102	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	1	0	contain	have	124:127	arg2	structures					147:156	structures	147:156	structures	147:156	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	3	1	from	substitution	505:516	arg1	carbon					553:558	the anomeric carbon	540:558	the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile)	540:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	2	2	theme	new	296:298	arg1	agents					312:317	new therapeutic agents	296:317	new therapeutic agents	296:317	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	5	3	attach	present	889:895	arg2	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	5	3	attach	present	889:895	arg1	group					912:916	the leaving group	900:916	the leaving group	900:916	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	14	4	theme	latter	2677:2682	arg1	intermediate					2697:2708	the latter vinylgold(I) intermediate	2673:2708	the latter vinylgold(I) intermediate	2673:2708	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	14	4	theme	latter	2677:2682	arg1	I					2694:2694	I	2694:2694	I	2694:2694	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	17	5	theme	future	3259:3264	arg1	developments					3266:3277	future developments	3259:3277	future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations	3259:3406	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	2	6	theme	those	279:283	arg1	interest					267:274	the interest	263:274	the interest of those developing new therapeutic agents	263:317	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	8	7	theme	side	1652:1655	arg1	reactions					1657:1665	few side reactions	1648:1665	few side reactions	1648:1665	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	10	8	theme	tetrahydrofuran	2192:2206	arg1	glycosylation					2056:2068	The direct glycosylation	2045:2068	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes,	2045:2140	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	8	theme	tetrahydrofuran	2192:2206	arg1	polymerization					2174:2187	the glycosylation-initiated polymerization	2146:2187	the glycosylation-initiated polymerization of tetrahydrofuran	2146:2206	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	3	9	theme	glycosyl	565:572	arg1	donor					574:578	a glycosyl donor	563:578	a glycosyl donor	563:578	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	16	10	with	C-Glycosylation	2932:2946	arg1	nucleophiles					2959:2970	silyl nucleophiles	2953:2970	silyl nucleophiles	2953:2970	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	10	11	theme	sugar	2128:2132	arg1	oximes					2134:2139	sugar oximes	2128:2139	sugar oximes	2128:2139	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	6	12	theme	reactions	1141:1149	arg1	development					1108:1118	the development	1104:1118	the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters	1104:1223	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	10	13	theme	direct	2049:2054	arg1	glycosylation					2056:2068	The direct glycosylation	2045:2068	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes,	2045:2140	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	13	14	theme	specific	2594:2601	arg1	cases					2603:2607	specific cases	2594:2607	specific cases	2594:2607	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	13	14	theme	specific	2594:2601	arg1	β-mannosylation/rhamnosylation					2618:2647	β-mannosylation/rhamnosylation	2618:2647	β-mannosylation/rhamnosylation	2618:2647	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	5	15	theme	nucleophilic	842:853	arg1	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	8	16	theme	new	1441:1443	arg1	protocol					1459:1466	this new glycosylation protocol	1436:1466	this new glycosylation protocol	1436:1466	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	5	17	theme	electrophilic	856:868	arg1	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	8	18	theme	protocol	1459:1466	arg1	merits					1426:1431	The merits	1422:1431	The merits of this new glycosylation protocol	1422:1466	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	16	19	theme	required	3089:3096	arg1	step					3114:3117	the required protodeauration step	3085:3117	the required protodeauration step	3085:3117	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	15	20	theme	acid	2819:2822	arg1	addition					2798:2805	the addition	2794:2805	the addition of a strong acid as a cocatalyst	2794:2838	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	7	21	theme	gold	1373:1376	arg1	catalysis					1381:1389	gold(I) catalysis	1373:1389	gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf	1373:1419	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	13	22	gly	glycosylations	2562:2575	arg1	cases					2603:2607	specific cases	2594:2607	specific cases	2594:2607	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	13	22	gly	glycosylations	2562:2575	arg1	β-mannosylation/rhamnosylation					2618:2647	β-mannosylation/rhamnosylation	2618:2647	β-mannosylation/rhamnosylation	2618:2647	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	14	23	theme	catalytic	2771:2779	arg1	cycle					2781:2785	the gold(I) catalytic cycle	2759:2785	the gold(I) catalytic cycle	2759:2785	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	12	24	dep	1-glycosyloxyisochromenylium-4-gold	2381:2415	arg1	I					2417:2417	I	2417:2417	I	2417:2417	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	5	25	theme	promoter	926:933	arg1	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	6	26	theme	conventional	1183:1194	arg1	use					1172:1174	the use	1168:1174	the use of the conventional leaving groups and promoters	1168:1223	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	10	27	theme	aglycones	2088:2096	arg1	glycosylation					2056:2068	The direct glycosylation	2045:2068	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes,	2045:2140	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	27	theme	aglycones	2088:2096	arg1	polymerization					2174:2187	the glycosylation-initiated polymerization	2146:2187	the glycosylation-initiated polymerization of tetrahydrofuran	2146:2206	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	13	28	theme	SN2-type	2553:2560	arg1	glycosylations					2562:2575	SN2-type glycosylations	2553:2575	SN2-type glycosylations	2553:2575	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	17	29	theme	glycosylation	3208:3220	arg1	method					3222:3227	the present glycosylation method	3196:3227	the present glycosylation method	3196:3227	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	5	30	theme	leaving	904:910	arg1	group					912:916	the leaving group	900:916	the leaving group	900:916	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	10	31	theme	dammarane	2107:2115	arg1	C20-ol					2117:2122	dammarane C20-ol	2107:2122	dammarane C20-ol	2107:2122	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	4	32	theme	unreactive	753:762	arg1	donors/acceptors					764:779	inherently unstable or unreactive donors/acceptors	730:779	inherently unstable or unreactive donors/acceptors	730:779	However, glycosylations involving inherently unstable or unreactive donors/acceptors are still problematic.					
29297680	13	33	theme	former	2512:2517	arg1	intermediate					2539:2550	the former glycosyloxypyrylium intermediate	2508:2550	the former glycosyloxypyrylium intermediate	2508:2550	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	3	34	theme	anomeric	544:551	arg1	carbon					553:558	the anomeric carbon	540:558	the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile)	540:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	8	35	theme	conventional	1741:1752	arg1	methods					1754:1760	conventional methods	1741:1760	conventional methods	1741:1760	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	1	36	theme	diverse	139:145	arg1	structures					147:156	structures	147:156	structures	147:156	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	glycoconjugates					1915:1929	glycoconjugates	1915:1929	glycoconjugates	1915:1929	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	peptides					2035:2042	peptides	2035:2042	peptides	2035:2042	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	nucleobases					1974:1984	nucleobases	1974:1984	nucleobases	1974:1984	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	variety					1884:1890	a wide variety	1877:1890	a wide variety	1877:1890	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	steroids					2008:2015	steroids	2008:2015	steroids	2008:2015	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	triterpenes					2018:2028	triterpenes	2018:2028	triterpenes	2018:2028	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	glycosides					1950:1959	complex glycosides	1942:1959	complex glycosides of epoxides	1942:1971	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	flavonoids					1987:1996	flavonoids	1987:1996	flavonoids	1987:1996	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	glycans					1903:1909	complex glycans	1895:1909	complex glycans	1895:1909	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	37	theme	glycoconjugates	1915:1929	arg1	lignans					1999:2005	lignans	1999:2005	lignans	1999:2005	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	3	38	theme	methods	448:454	arg1	array					425:429	a wide array	418:429	a wide array of glycosylation methods	418:454	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	6	39	theme	gold	1083:1086	arg1	catalysts					1091:1099	alkynophilic gold(I) catalysts	1070:1099	alkynophilic gold(I) catalysts	1070:1099	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	11	40	theme	glycosylation	2285:2297	arg1	protocol					2299:2306	the present glycosylation protocol	2273:2306	the present glycosylation protocol	2273:2306	The gold(I) catalytic cycle of the present glycosylation protocol has been fully elucidated.					
29297680	16	41	theme	H+	3072:3073	arg1	moisture					3003:3010	moisture	3003:3010	moisture	3003:3010	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	16	41	theme	H+	3072:3073	arg1	donor					3075:3079	the H+ donor	3068:3079	the H+ donor for the required protodeauration step	3068:3117	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	1	42	theme	occurring	86:94	arg1	glycans					96:102	Naturally occurring glycans	76:102	Naturally occurring glycans	76:102	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	17	43	theme	catalytic	3369:3377	arg1	efficiency					3379:3388	catalytic efficiency	3369:3388	catalytic efficiency	3369:3388	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	4	44	theme	unstable	741:748	arg1	donors/acceptors					764:779	inherently unstable or unreactive donors/acceptors	730:779	inherently unstable or unreactive donors/acceptors	730:779	However, glycosylations involving inherently unstable or unreactive donors/acceptors are still problematic.					
29297680	9	45	theme	wide	1879:1882	arg1	glycoconjugates					1915:1929	glycoconjugates	1915:1929	glycoconjugates	1915:1929	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	peptides					2035:2042	peptides	2035:2042	peptides	2035:2042	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	nucleobases					1974:1984	nucleobases	1974:1984	nucleobases	1974:1984	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	variety					1884:1890	a wide variety	1877:1890	a wide variety	1877:1890	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	steroids					2008:2015	steroids	2008:2015	steroids	2008:2015	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	triterpenes					2018:2028	triterpenes	2018:2028	triterpenes	2018:2028	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	glycosides					1950:1959	complex glycosides	1942:1959	complex glycosides of epoxides	1942:1971	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	flavonoids					1987:1996	flavonoids	1987:1996	flavonoids	1987:1996	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	glycans					1903:1909	complex glycans	1895:1909	complex glycans	1895:1909	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	45	theme	wide	1879:1882	arg1	lignans					1999:2005	lignans	1999:2005	lignans	1999:2005	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	6	46	theme	developed	1047:1055	arg1	chemistry					1057:1065	the recently developed chemistry	1034:1065	the recently developed chemistry of alkynophilic gold(I) catalysts	1034:1099	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	3	47	theme	wide	420:423	arg1	array					425:429	a wide array	418:429	a wide array of glycosylation methods	418:454	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	9	48	theme	epoxides	1964:1971	arg1	peptides					2035:2042	peptides	2035:2042	peptides	2035:2042	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	nucleobases					1974:1984	nucleobases	1974:1984	nucleobases	1974:1984	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	steroids					2008:2015	steroids	2008:2015	steroids	2008:2015	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	triterpenes					2018:2028	triterpenes	2018:2028	triterpenes	2018:2028	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	glycosides					1950:1959	complex glycosides	1942:1959	complex glycosides of epoxides	1942:1971	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	flavonoids					1987:1996	flavonoids	1987:1996	flavonoids	1987:1996	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	48	theme	epoxides	1964:1971	arg1	lignans					1999:2005	lignans	1999:2005	lignans	1999:2005	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	0	49	theme	-Catalyzed	7:16	arg1	Glycosylation					18:30	Gold(I)-Catalyzed Glycosylation	0:30	Gold(I)-Catalyzed Glycosylation with Glycosyl	0:44	Gold(I)-Catalyzed Glycosylation with Glycosyl o-Alkynylbenzoates as Donors.					
29297680	8	50	dep	include	1468:1474	arg1	1					1492:1492	1	1492:1492	1	1492:1492	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	50	dep	include	1468:1474	arg1	observed					1671:1678	observed	1671:1678	are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple	1667:1804	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	50	dep	include	1468:1474	arg1	scope					1608:1612	donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope	1499:1612	the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope	1495:1612	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	50	dep	include	1468:1474	arg1	wide					1627:1630	wide	1627:1630	wide	1627:1630	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	50	dep	include	1468:1474	arg1	4					1634:1634	4	1634:1634	4	1634:1634	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	51	dep	orthogonal	1718:1727	arg1	conditions					1703:1712	the glycosylation conditions	1685:1712	the glycosylation conditions	1685:1712	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	51	dep	orthogonal	1718:1727	arg1	5					1682:1682	5	1682:1682	5	1682:1682	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	51	dep	orthogonal	1718:1727	arg1	orthogonal					1718:1727	orthogonal	1718:1727	orthogonal	1718:1727	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	catalytic					1579:1587	catalytic	1579:1587	catalytic	1579:1587	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	prepared					1517:1524	prepared	1517:1524	are easily prepared	1506:1524	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	2					1559:1559	2	1559:1559	2	1559:1559	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	3					1591:1591	3	1591:1591	3	1591:1591	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	shelf-stable					1544:1555	shelf-stable	1544:1555	shelf-stable	1544:1555	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	52	dep	scope	1608:1612	arg1	promotion					1566:1574	the promotion	1562:1574	the promotion	1562:1574	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	14	53	theme	vinylgold	2684:2692	arg1	intermediate					2697:2708	the latter vinylgold(I) intermediate	2673:2708	the latter vinylgold(I) intermediate	2673:2708	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	14	53	theme	vinylgold	2684:2692	arg1	I					2694:2694	I	2694:2694	I	2694:2694	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	2	54	theme	molecules	203:211	arg1	mimics					234:239	their artificial mimics	217:239	their artificial mimics	217:239	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	2	54	theme	molecules	203:211	arg1	Syntheses					184:192	Syntheses	184:192	Syntheses of these molecules	184:211	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	6	55	theme	catalysts	1091:1099	arg1	chemistry					1057:1065	the recently developed chemistry	1034:1065	the recently developed chemistry of alkynophilic gold(I) catalysts	1034:1099	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	11	56	theme	gold	2246:2249	arg1	cycle					2264:2268	The gold(I) catalytic cycle	2242:2268	The gold(I) catalytic cycle of the present glycosylation protocol	2242:2306	The gold(I) catalytic cycle of the present glycosylation protocol has been fully elucidated.					
29297680	2	57	theme	various	373:379	arg1	linkages					392:399	the various glycosidic linkages	369:399	the various glycosidic linkages	369:399	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	10	58	theme	glycosylation-initiated	2150:2172	arg1	polymerization					2174:2187	the glycosylation-initiated polymerization	2146:2187	the glycosylation-initiated polymerization of tetrahydrofuran	2146:2206	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	7	59	theme	glycosyl	1240:1247	arg1	o-alkynylbenzoates					1249:1266	glycosyl o-alkynylbenzoates	1240:1266	glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates)	1240:1330	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	10	60	gly	glycosylation	2056:2068	arg1	tetrahydrofuran					2192:2206	tetrahydrofuran	2192:2206	tetrahydrofuran	2192:2206	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	60	gly	glycosylation	2056:2068	arg1	C20-ol					2117:2122	dammarane C20-ol	2107:2122	dammarane C20-ol	2107:2122	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	60	gly	glycosylation	2056:2068	arg1	aglycones					2088:2096	some sensitive aglycones	2073:2096	some sensitive aglycones	2073:2096	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	60	gly	glycosylation	2056:2068	arg1	oximes					2134:2139	sugar oximes	2128:2139	sugar oximes	2128:2139	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	2	61	theme	therapeutic	300:310	arg1	agents					312:317	new therapeutic agents	296:317	new therapeutic agents	296:317	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	3	62	dep	promoter	646:653	arg1	electrophile					681:692	a stoichiometric electrophile	664:692	usually a stoichiometric electrophile	656:692	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	7	63	theme	glycosyl	1277:1284	arg1	o-hexynyl-					1286:1295	glycosyl o-hexynyl-	1277:1295	glycosyl o-hexynyl-	1277:1295	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	3	64	theme	group	531:535	arg1	substitution					505:516	the substitution	501:516	the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile)	501:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	14	65	theme	gold	2763:2766	arg1	cycle					2781:2785	the gold(I) catalytic cycle	2759:2785	the gold(I) catalytic cycle	2759:2785	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	12	66	theme	1-glycosyloxyisochromenylium-4-gold	2381:2415	arg1	complexes					2447:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	8	67	theme	few	1648:1650	arg1	reactions					1657:1665	few side reactions	1648:1665	few side reactions	1648:1665	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	5	68	theme	acidic	874:879	arg1	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	3	69	theme	promoter	646:653	arg1	action					623:628	the action	619:628	the action of a particular promoter (usually a stoichiometric electrophile)	619:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	12	70	theme	key	2350:2352	arg1	intermediates					2354:2366	key intermediates	2350:2366	key intermediates	2350:2366	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	12	70	theme	key	2350:2352	arg1	complexes					2447:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	17	71	theme	glycosylation	3282:3294	arg1	methodologies					3296:3308	glycosylation methodologies	3282:3308	glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations	3282:3406	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	11	72	theme	catalytic	2254:2262	arg1	cycle					2264:2268	The gold(I) catalytic cycle	2242:2268	The gold(I) catalytic cycle of the present glycosylation protocol	2242:2306	The gold(I) catalytic cycle of the present glycosylation protocol has been fully elucidated.					
29297680	10	73	theme	first	2230:2234	arg1	time					2236:2239	the first time	2226:2239	the first time	2226:2239	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	8	74	theme	glycosylation	1445:1457	arg1	protocol					1459:1466	this new glycosylation protocol	1436:1466	this new glycosylation protocol	1436:1466	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	6	75	theme	glycosylation	1127:1139	arg1	reactions					1141:1149	new glycosylation reactions	1123:1149	new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters	1123:1223	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	17	76	dep	diastereoselectivity	3344:3363	arg1	the					3340:3342	the	3340:3342	the	3340:3342	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	3	77	theme	donor	574:578	arg1	carbon					553:558	the anomeric carbon	540:558	the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile)	540:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	11	78	theme	protocol	2299:2306	arg1	cycle					2264:2268	The gold(I) catalytic cycle	2242:2268	The gold(I) catalytic cycle of the present glycosylation protocol	2242:2306	The gold(I) catalytic cycle of the present glycosylation protocol has been fully elucidated.					
29297680	17	79	theme	broad	3173:3177	arg1	applicability					3179:3191	broad applicability	3173:3191	broad applicability	3173:3191	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	9	80	theme	glycans	1903:1909	arg1	glycoconjugates					1915:1929	glycoconjugates	1915:1929	glycoconjugates	1915:1929	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	peptides					2035:2042	peptides	2035:2042	peptides	2035:2042	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	nucleobases					1974:1984	nucleobases	1974:1984	nucleobases	1974:1984	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	variety					1884:1890	a wide variety	1877:1890	a wide variety	1877:1890	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	steroids					2008:2015	steroids	2008:2015	steroids	2008:2015	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	triterpenes					2018:2028	triterpenes	2018:2028	triterpenes	2018:2028	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	glycosides					1950:1959	complex glycosides	1942:1959	complex glycosides of epoxides	1942:1971	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	flavonoids					1987:1996	flavonoids	1987:1996	flavonoids	1987:1996	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	glycans					1903:1909	complex glycans	1895:1909	complex glycans	1895:1909	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	9	80	theme	glycans	1903:1909	arg1	lignans					1999:2005	lignans	1999:2005	lignans	1999:2005	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	14	81	theme	intermediate	2697:2708	arg1	protodeauration					2654:2668	The protodeauration	2650:2668	The protodeauration of the latter vinylgold(I) intermediate	2650:2708	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	14	81	theme	intermediate	2697:2708	arg1	important					2745:2753	important	2745:2753	important	2745:2753	The protodeauration of the latter vinylgold(I) intermediate has been reported to be critically important for the gold(I) catalytic cycle.					
29297680	17	82	dep	merits	3162:3167	arg1	the					3158:3160	the	3158:3160	the	3158:3160	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	16	83	theme	silyl	2953:2957	arg1	nucleophiles					2959:2970	silyl nucleophiles	2953:2970	silyl nucleophiles	2953:2970	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	8	84	theme	glycosylation	1689:1701	arg1	conditions					1703:1712	the glycosylation conditions	1685:1712	the glycosylation conditions	1685:1712	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	84	theme	glycosylation	1689:1701	arg1	5					1682:1682	5	1682:1682	5	1682:1682	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	84	theme	glycosylation	1689:1701	arg1	orthogonal					1718:1727	orthogonal	1718:1727	orthogonal	1718:1727	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	5	85	theme	present	889:895	arg1	species					881:887	nucleophilic, electrophilic, or acidic species	842:887	nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter	842:933	In those systems, reactions involving nucleophilic, electrophilic, or acidic species present on the leaving group and the promoter could become competitive and detrimental to the glycosylation.					
29297680	8	86	theme	substrate	1598:1606	arg1	scope					1608:1612	donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope	1499:1612	the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope	1495:1612	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	86	theme	substrate	1598:1606	arg1	wide					1627:1630	wide	1627:1630	wide	1627:1630	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	87	dep	simple	1799:1804	arg1	6					1768:1768	6	1768:1768	6	1768:1768	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	87	dep	simple	1799:1804	arg1	method					1775:1780	the method	1771:1780	the method	1771:1780	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	87	dep	simple	1799:1804	arg1	simple					1799:1804	simple	1799:1804	simple	1799:1804	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	15	88	theme	strong	2812:2817	arg1	acid					2819:2822	a strong acid	2810:2822	a strong acid	2810:2822	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	10	89	theme	sensitive	2078:2086	arg1	oximes					2134:2139	sugar oximes	2128:2139	sugar oximes	2128:2139	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	89	theme	sensitive	2078:2086	arg1	C20-ol					2117:2122	dammarane C20-ol	2107:2122	dammarane C20-ol	2107:2122	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	10	89	theme	sensitive	2078:2086	arg1	aglycones					2088:2096	some sensitive aglycones	2073:2096	some sensitive aglycones	2073:2096	The direct glycosylation of some sensitive aglycones, such as dammarane C20-ol and sugar oximes, and the glycosylation-initiated polymerization of tetrahydrofuran were achieved for the first time.					
29297680	17	90	theme	present	3200:3206	arg1	method					3222:3227	the present glycosylation method	3196:3227	the present glycosylation method	3196:3227	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	1	91	theme	biological	162:171	arg1	functions					173:181	biological functions	162:181	biological functions	162:181	Naturally occurring glycans and glycoconjugates have extremely diverse structures and biological functions.					
29297680	9	92	theme	complex	1895:1901	arg1	glycans					1903:1909	complex glycans	1895:1909	complex glycans	1895:1909	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	17	93	theme	method	3222:3227	arg1	merits					3162:3167	merits	3162:3167	merits	3162:3167	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	17	93	theme	method	3222:3227	arg1	applicability					3179:3191	broad applicability	3173:3191	broad applicability	3173:3191	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	16	94	theme	molecular	3037:3045	arg1	sieves					3047:3052	molecular sieves	3037:3052	molecular sieves	3037:3052	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	9	95	theme	variety	1884:1890	arg1	synthesis					1864:1872	the synthesis	1860:1872	the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides	1860:2042	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	7	96	with	catalysis	1381:1389	arg1	Ph3PAuOTf					1411:1419	Ph3PAuOTf	1411:1419	Ph3PAuOTf	1411:1419	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	7	96	with	catalysis	1381:1389	arg1	Ph3PAuNTf2					1396:1405	Ph3PAuNTf2	1396:1405	Ph3PAuNTf2	1396:1405	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	8	97	dep	catalytic	1579:1587	arg1	catalytic					1579:1587	catalytic	1579:1587	catalytic	1579:1587	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	97	dep	catalytic	1579:1587	arg1	3					1591:1591	3	1591:1591	3	1591:1591	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	97	dep	catalytic	1579:1587	arg1	promotion					1566:1574	the promotion	1562:1574	the promotion	1562:1574	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	8	97	dep	catalytic	1579:1587	arg1	2					1559:1559	2	1559:1559	2	1559:1559	The merits of this new glycosylation protocol include the following: (1) the donors are easily prepared and are generally shelf-stable; (2) the promotion is catalytic; (3) the substrate scope is extremely wide; (4) relatively few side reactions are observed; (5) the glycosylation conditions are orthogonal to those of conventional methods; and (6) the method is operationally simple.					
29297680	15	98	dep	0.001	2918:2922	arg1	to					2915:2916	to	2915:2916	to	2915:2916	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	15	99	theme	gold	2892:2895	arg1	catalyst					2900:2907	the gold(I) catalyst	2888:2907	the gold(I) catalyst (down to 0.001 equiv)	2888:2929	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	15	99	theme	gold	2892:2895	arg1	I					2897:2897	I	2897:2897	I	2897:2897	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	15	99	theme	gold	2892:2895	arg1	equiv					2924:2928	down to 0.001 equiv	2910:2928	down to 0.001 equiv	2910:2928	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	17	100	from	developments	3266:3277	arg1	methodologies					3296:3308	glycosylation methodologies	3282:3308	glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations	3282:3406	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	11	101	theme	present	2277:2283	arg1	protocol					2299:2306	the present glycosylation protocol	2273:2306	the present glycosylation protocol	2273:2306	The gold(I) catalytic cycle of the present glycosylation protocol has been fully elucidated.					
29297680	17	102	theme	unique	3132:3137	arg1	mechanism					3139:3147	the unique mechanism	3128:3147	the unique mechanism	3128:3147	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	13	103	theme	glycosyloxypyrylium	2519:2537	arg1	intermediate					2539:2550	the former glycosyloxypyrylium intermediate	2508:2550	the former glycosyloxypyrylium intermediate	2508:2550	Exploiting the former glycosyloxypyrylium intermediate, SN2-type glycosylations were realized in specific cases, such as β-mannosylation/rhamnosylation.					
29297680	3	104	with	carbon	553:558	arg1	acceptor					588:595	an acceptor	585:595	an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile)	585:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	3	104	with	carbon	553:558	arg1	nucleophile					600:610	a nucleophile	598:610	a nucleophile	598:610	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	6	105	theme	alkynophilic	1070:1081	arg1	I					1088:1088	I	1088:1088	I	1088:1088	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	6	105	theme	alkynophilic	1070:1081	arg1	gold					1083:1086	alkynophilic gold	1070:1086	alkynophilic gold(I) catalysts	1070:1099	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	3	106	theme	particular	635:644	arg1	promoter					646:653	a particular promoter	633:653	a particular promoter (usually a stoichiometric electrophile)	633:693	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	9	107	theme	complex	1942:1948	arg1	glycosides					1950:1959	complex glycosides	1942:1959	complex glycosides of epoxides	1942:1971	Indeed, this method has been successfully applied in the synthesis of a wide variety of complex glycans and glycoconjugates, including complex glycosides of epoxides, nucleobases, flavonoids, lignans, steroids, triterpenes, and peptides.					
29297680	3	108	theme	glycosylation	434:446	arg1	methods					448:454	glycosylation methods	434:454	glycosylation methods	434:454	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	12	109	dep	isochromen-4-ylgold	2424:2442	arg1	I					2444:2444	I	2444:2444	I	2444:2444	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	7	110	dep	o-alkynylbenzoates	1249:1266	arg1	o-cyclopropylethynylbenzoates					1301:1329	o-cyclopropylethynylbenzoates	1301:1329	o-cyclopropylethynylbenzoates	1301:1329	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	7	110	dep	o-alkynylbenzoates	1249:1266	arg1	o-hexynyl-					1286:1295	glycosyl o-hexynyl-	1277:1295	glycosyl o-hexynyl-	1277:1295	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	15	111	theme	required	2868:2875	arg1	loading					2877:2883	the required loading	2864:2883	the required loading of the gold(I) catalyst (down to 0.001 equiv)	2864:2929	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	16	112	theme	protodeauration	3098:3112	arg1	step					3114:3117	the required protodeauration step	3085:3117	the required protodeauration step	3085:3117	C-Glycosylation with silyl nucleophiles can proceed catalytically when moisture, which is sequestered by molecular sieves, can serve as the H+ donor for the required protodeauration step.					
29297680	17	113	theme	glycosylations	3393:3406	arg1	diastereoselectivity					3344:3363	diastereoselectivity	3344:3363	diastereoselectivity	3344:3363	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	17	113	theme	glycosylations	3393:3406	arg1	efficiency					3379:3388	catalytic efficiency	3369:3388	catalytic efficiency	3369:3388	Indeed, the unique mechanism explains the merits and broad applicability of the present glycosylation method and provides a foundation for future developments in glycosylation methodologies that mainly involve improving the diastereoselectivity and catalytic efficiency of glycosylations.					
29297680	6	114	theme	new	1123:1125	arg1	reactions					1141:1149	new glycosylation reactions	1123:1149	new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters	1123:1223	To address this problem, we applied the recently developed chemistry of alkynophilic gold(I) catalysts to the development of new glycosylation reactions that would avoid the use of the conventional leaving groups and promoters.					
29297680	2	115	theme	glycosidic	381:390	arg1	linkages					392:399	the various glycosidic linkages	369:399	the various glycosidic linkages	369:399	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	2	116	theme	artificial	223:232	arg1	mimics					234:239	their artificial mimics	217:239	their artificial mimics	217:239	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	12	117	theme	isochromen-4-ylgold	2424:2442	arg1	complexes					2447:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes	2377:2455	In particular, key intermediates, such as the 1-glycosyloxyisochromenylium-4-gold(I) and isochromen-4-ylgold(I) complexes, have been unambiguously characterized.					
29297680	2	118	theme	glycosylation	328:340	arg1	methodologies					342:354	glycosylation methodologies	328:354	glycosylation methodologies	328:354	Syntheses of these molecules and their artificial mimics, which have attracted the interest of those developing new therapeutic agents, rely on glycosylation methodologies to construct the various glycosidic linkages.					
29297680	15	119	theme	catalyst	2900:2907	arg1	loading					2877:2883	the required loading	2864:2883	the required loading of the gold(I) catalyst (down to 0.001 equiv)	2864:2929	Thus, the addition of a strong acid as a cocatalyst can dramatically reduce the required loading of the gold(I) catalyst (down to 0.001 equiv).					
29297680	7	120	theme	privileged	1349:1358	arg1	donors					1360:1365	privileged donors	1349:1365	privileged donors	1349:1365	Gratifyingly, glycosyl o-alkynylbenzoates (namely, glycosyl o-hexynyl- and o-cyclopropylethynylbenzoates) turned out to be privileged donors under gold(I) catalysis with Ph3PAuNTf2 and Ph3PAuOTf.					
29297680	3	121	theme	stoichiometric	666:679	arg1	electrophile					681:692	a stoichiometric electrophile	664:692	usually a stoichiometric electrophile	656:692	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
29297680	0	122	with	Glycosylation	18:30	arg1	Glycosyl					37:44	Glycosyl	37:44	Glycosyl	37:44	Gold(I)-Catalyzed Glycosylation with Glycosyl o-Alkynylbenzoates as Donors.					
29297680	3	123	theme	leaving	523:529	arg1	group					531:535	a leaving group	521:535	a leaving group	521:535	In this regard, a wide array of glycosylation methods have been developed, and they mainly involve the substitution of a leaving group on the anomeric carbon of a glycosyl donor with an acceptor (a nucleophile) under the action of a particular promoter (usually a stoichiometric electrophile).					
30824758	0	0	theme	environmental	80:92	arg1	conditions					94:103	environmental conditions	80:103	environmental conditions	80:103	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	3	1	theme	β-GlcNAc	681:688	arg1	substitutions					699:711	the α- and β-GlcNAc anomeric substitutions	670:711	the α- and β-GlcNAc anomeric substitutions	670:711	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	1	2	with	constituents	141:152	arg1	roles					221:225	important roles	211:225	important roles in the bacteria's physiology	211:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	7	3	theme	aureus	1202:1207	arg1	strain					1216:1221	prototype S. aureus Newman strain	1189:1221	the prototype S. aureus Newman strain	1185:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	8	4	theme	complete	1412:1419	arg1	switch					1421:1426	a complete switch	1410:1426	a complete switch to β-glycosylation	1410:1445	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	5	5	theme	β-GlcNAc	891:898	arg1	form					904:907	the β-GlcNAc WTA form	887:907	the β-GlcNAc WTA form	887:907	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	7	6	theme	prototype	1189:1197	arg1	strain					1216:1221	prototype S. aureus Newman strain	1189:1221	the prototype S. aureus Newman strain	1185:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	1	7	theme	biofilm	328:334	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	7	theme	biofilm	328:334	arg1	formation					336:344	biofilm formation	328:344	biofilm formation	328:344	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	9	8	theme	environmental	1590:1602	arg1	conditions					1604:1613	environmental conditions	1590:1613	environmental conditions	1590:1613	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	3	9	theme	aureus	514:519	arg1	pathways					538:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	6	10	theme	high	1126:1129	arg1	concentration					1136:1148	high NaCl concentration	1126:1148	high NaCl concentration	1126:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	7	11	theme	S.	1199:1200	arg1	strain					1216:1221	prototype S. aureus Newman strain	1189:1221	the prototype S. aureus Newman strain	1185:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	5	12	theme	tarS	948:951	arg1	gene					953:956	the tarS gene	944:956	the tarS gene in all strains assessed	944:980	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	3	13	theme	WTA	521:523	arg1	pathways					538:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	7	14	theme	different	1256:1264	arg1	peritonitis					1288:1298	peritonitis	1288:1298	peritonitis	1288:1298	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	14	theme	different	1256:1264	arg1	infection					1315:1323	deep wound infection	1304:1323	deep wound infection	1304:1323	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	14	theme	different	1256:1264	arg1	models					1273:1278	two different animal models	1252:1278	two different animal models	1252:1278	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	15	theme	animal	1266:1271	arg1	peritonitis					1288:1298	peritonitis	1288:1298	peritonitis	1288:1298	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	15	theme	animal	1266:1271	arg1	infection					1315:1323	deep wound infection	1304:1323	deep wound infection	1304:1323	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	15	theme	animal	1266:1271	arg1	models					1273:1278	two different animal models	1252:1278	two different animal models	1252:1278	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	6	16	theme	α-GlcNAc	1054:1061	arg1	anomer					1063:1068	the α-GlcNAc anomer	1050:1068	the α-GlcNAc anomer	1050:1068	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	3	17	theme	complete	502:509	arg1	pathways					538:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	5	18	theme	S.	860:861	arg1	strains					870:876	the S. aureus strains	856:876	the S. aureus strains	856:876	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	2	19	theme	repeat	381:386	arg1	units					388:392	ribitol phosphate repeat units	363:392	ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc)	363:495	They consist of ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc).					
30824758	3	20	theme	anomeric	690:697	arg1	substitutions					699:711	the α- and β-GlcNAc anomeric substitutions	670:711	the α- and β-GlcNAc anomeric substitutions	670:711	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	6	21	theme	NaCl	1131:1134	arg1	concentration					1136:1148	high NaCl concentration	1126:1148	high NaCl concentration	1126:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	9	22	theme	aureus	1539:1544	arg1	glycosylation					1550:1562	S. aureus WTA glycosylation	1536:1562	S. aureus WTA glycosylation	1536:1562	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	6	23	theme	anomer	1063:1068	arg1	expense					1039:1045	the expense	1035:1045	the expense of the α-GlcNAc anomer	1035:1068	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	1	24	theme	aureus	172:177	arg1	envelopes					196:204	Staphylococcus aureus (S. aureus) cell envelopes	157:204	Staphylococcus aureus (S. aureus) cell envelopes	157:204	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	7	25	theme	WTA	1164:1166	arg1	glycosylation					1168:1180	WTA glycosylation	1164:1180	WTA glycosylation of the prototype S. aureus Newman strain	1164:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	26	theme	Newman	1209:1214	arg1	strain					1216:1221	prototype S. aureus Newman strain	1189:1221	the prototype S. aureus Newman strain	1185:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	4	27	theme	structural	727:736	arg1	analyses					738:745	structural analyses	727:745	structural analyses	727:745	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	5	28	from	strains	965:971	arg1	presence					932:939	the presence	928:939	the presence of the tarS gene in all strains assessed	928:980	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	2	29	theme	ribitol	363:369	arg1	units					388:392	ribitol phosphate repeat units	363:392	ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc)	363:495	They consist of ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc).					
30824758	9	30	theme	WTA	1546:1548	arg1	glycosylation					1550:1562	S. aureus WTA glycosylation	1536:1562	S. aureus WTA glycosylation	1536:1562	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	8	31	theme	infected	1463:1470	arg1	kidneys					1472:1478	infected kidneys	1463:1478	infected kidneys	1463:1478	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	5	32	theme	aureus	863:868	arg1	strains					870:876	the S. aureus strains	856:876	the S. aureus strains	856:876	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	0	33	theme	aureus	32:37	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	4	34	theme	aureus	790:795	arg1	strains					797:803	24 S. aureus strains	784:803	24 S. aureus strains responsible for invasive infections	784:839	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	8	35	theme	α-GlcNAc	1396:1403	arg1	WTA					1405:1407	α-GlcNAc WTA	1396:1407	α-GlcNAc WTA	1396:1407	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	1	36	theme	Wall	106:109	arg1	WTA					126:128	WTA	126:128	WTA	126:128	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	36	theme	Wall	106:109	arg1	acid					120:123	Wall teichoic acid	106:123	Wall teichoic acid (WTA)	106:129	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	36	theme	Wall	106:109	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	4	37	theme	responsible	805:815	arg1	strains					797:803	24 S. aureus strains	784:803	24 S. aureus strains responsible for invasive infections	784:839	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	0	38	theme	Staphylococcus	17:30	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	5	39	theme	gene	953:956	arg1	presence					932:939	the presence	928:939	the presence of the tarS gene in all strains assessed	928:980	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	1	40	theme	teichoic	111:118	arg1	WTA					126:128	WTA	126:128	WTA	126:128	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	40	theme	teichoic	111:118	arg1	acid					120:123	Wall teichoic acid	106:123	Wall teichoic acid (WTA)	106:129	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	40	theme	teichoic	111:118	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	0	41	theme	wall	44:47	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	1	42	theme	antimicrobial	271:283	arg1	molecules					285:293	antimicrobial molecules	271:293	antimicrobial molecules	271:293	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	0	43	gly	Glycosylation	0:12	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	7	44	theme	wound	1309:1313	arg1	infection					1315:1323	deep wound infection	1304:1323	deep wound infection	1304:1323	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	44	theme	wound	1309:1313	arg1	models					1273:1278	two different animal models	1252:1278	two different animal models	1252:1278	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	1	45	theme	cell	191:194	arg1	envelopes					196:204	Staphylococcus aureus (S. aureus) cell envelopes	157:204	Staphylococcus aureus (S. aureus) cell envelopes	157:204	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	0	46	theme	cell	39:42	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	4	47	theme	strains	797:803	arg1	panel					775:779	a panel	773:779	a panel of 24 S. aureus strains responsible for invasive infections	773:839	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	1	48	theme	envelopes	196:204	arg1	formation					336:344	biofilm formation	328:344	biofilm formation	328:344	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	48	theme	envelopes	196:204	arg1	interaction					301:311	host interaction	296:311	host interaction	296:311	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	48	theme	envelopes	196:204	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	48	theme	envelopes	196:204	arg1	resistance					257:266	resistance	257:266	resistance to antimicrobial molecules	257:293	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	48	theme	envelopes	196:204	arg1	acid					120:123	Wall teichoic acid	106:123	Wall teichoic acid (WTA)	106:129	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	48	theme	envelopes	196:204	arg1	virulence					314:322	virulence	314:322	virulence	314:322	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	0	49	theme	acid	58:61	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of Staphylococcus aureus cell wall teichoic acid	0:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	1	50	from	roles	221:225	arg1	physiology					245:254	the bacteria's physiology	230:254	the bacteria's physiology	230:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	6	51	contain	containing	1115:1124	arg1	medium					1108:1113	stress-inducing culture medium	1084:1113	stress-inducing culture medium containing high NaCl concentration	1084:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	6	51	contain	containing	1115:1124	arg2	concentration					1136:1148	high NaCl concentration	1126:1148	high NaCl concentration	1126:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	4	52	theme	invasive	821:828	arg1	infections					830:839	invasive infections	821:839	invasive infections	821:839	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	5	53	theme	WTA	900:902	arg1	form					904:907	the β-GlcNAc WTA form	887:907	the β-GlcNAc WTA form	887:907	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	0	54	theme	teichoic	49:56	arg1	acid					58:61	Staphylococcus aureus cell wall teichoic acid	17:61	Staphylococcus aureus cell wall teichoic acid	17:61	Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions.					
30824758	1	55	theme	host	296:299	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	55	theme	host	296:299	arg1	interaction					301:311	host interaction	296:311	host interaction	296:311	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	3	56	theme	responsible	654:664	arg1	TarS					635:638	TarS	635:638	TarS	635:638	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	3	56	theme	responsible	654:664	arg1	TarM					626:629	TarM	626:629	TarM	626:629	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	3	56	theme	responsible	654:664	arg1	glycosyltransferases					604:623	the two glycosyltransferases	596:623	the two glycosyltransferases	596:623	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	1	57	dep	aureus	172:177	arg1	aureus					183:188	S. aureus	180:188	S. aureus	180:188	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	9	58	theme	competitive	1655:1665	arg1	advantage					1667:1675	competitive advantage	1655:1675	competitive advantage	1655:1675	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	5	59	from	presence	932:939	arg1	strains					965:971	all strains	961:971	all strains assessed	961:980	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	4	60	theme	24 S.	784:788	arg1	strains					797:803	24 S. aureus strains	784:803	24 S. aureus strains responsible for invasive infections	784:839	We performed structural analyses to characterize WTAs from a panel of 24 S. aureus strains responsible for invasive infections.					
30824758	7	61	gly	glycosylation	1168:1180	arg1	peritonitis					1288:1298	peritonitis	1288:1298	peritonitis	1288:1298	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	61	gly	glycosylation	1168:1180	arg1	infection					1315:1323	deep wound infection	1304:1323	deep wound infection	1304:1323	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	61	gly	glycosylation	1168:1180	arg1	strain					1216:1221	prototype S. aureus Newman strain	1189:1221	the prototype S. aureus Newman strain	1185:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	61	gly	glycosylation	1168:1180	arg1	models					1273:1278	two different animal models	1252:1278	two different animal models	1252:1278	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	9	62	theme	β-GlcNAc	1632:1639	arg1	WTA					1641:1643	β-GlcNAc WTA	1632:1643	β-GlcNAc WTA	1632:1643	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	6	63	theme	culture	1100:1106	arg1	medium					1108:1113	stress-inducing culture medium	1084:1113	stress-inducing culture medium containing high NaCl concentration	1084:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	3	64	theme	biosynthesis	525:536	arg1	pathways					538:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways	498:545	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	3	65	theme	glycosyltransferases	604:623	arg1	identification					578:591	the identification	574:591	the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions	574:711	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	6	66	theme	stress-inducing	1084:1098	arg1	medium					1108:1113	stress-inducing culture medium	1084:1113	stress-inducing culture medium containing high NaCl concentration	1084:1148	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	7	67	theme	deep	1304:1307	arg1	infection					1315:1323	deep wound infection	1304:1323	deep wound infection	1304:1323	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	7	67	theme	deep	1304:1307	arg1	models					1273:1278	two different animal models	1252:1278	two different animal models	1252:1278	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	1	68	theme	important	211:219	arg1	roles					221:225	important roles	211:225	important roles in the bacteria's physiology	211:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	7	69	theme	strain	1216:1221	arg1	glycosylation					1168:1180	WTA glycosylation	1164:1180	WTA glycosylation of the prototype S. aureus Newman strain	1164:1221	Furthermore, WTA glycosylation of the prototype S. aureus Newman strain was characterized in vivo in two different animal models, namely peritonitis and deep wound infection.					
30824758	8	70	located	observed	1451:1458	arg2	switch					1421:1426	a complete switch	1410:1426	a complete switch to β-glycosylation	1410:1445	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	8	70	located	observed	1451:1458	arg1	muscles					1492:1498	muscles	1492:1498	muscles	1492:1498	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	8	70	located	observed	1451:1458	arg1	livers					1481:1486	livers	1481:1486	livers	1481:1486	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	8	70	located	observed	1451:1458	arg1	kidneys					1472:1478	infected kidneys	1463:1478	infected kidneys	1463:1478	While the inoculum used to infect animals produced almost exclusively α-GlcNAc WTA, a complete switch to β-glycosylation was observed in infected kidneys, livers and muscles.					
30824758	3	71	theme	α-	674:675	arg1	substitutions					699:711	the α- and β-GlcNAc anomeric substitutions	670:711	the α- and β-GlcNAc anomeric substitutions	670:711	The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions.					
30824758	5	72	theme	strains	870:876	arg1	majority					844:851	A majority	842:851	A majority of the S. aureus strains	842:876	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
30824758	2	73	theme	phosphate	371:379	arg1	units					388:392	ribitol phosphate repeat units	363:392	ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc)	363:495	They consist of ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc).					
30824758	6	74	theme	β-GlcNAc	987:994	arg1	anomer					996:1001	The β-GlcNAc anomer	983:1001	The β-GlcNAc anomer	983:1001	The β-GlcNAc anomer was preferentially expressed at the expense of the α-GlcNAc anomer when grown on stress-inducing culture medium containing high NaCl concentration.					
30824758	2	75	theme	ribitol	407:413	arg1	residue					415:421	the ribitol residue	403:421	the ribitol residue	403:421	They consist of ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc).					
30824758	9	76	theme	S.	1536:1537	arg1	glycosylation					1550:1562	S. aureus WTA glycosylation	1536:1562	S. aureus WTA glycosylation	1536:1562	Overall, our data demonstrate that S. aureus WTA glycosylation is strongly influenced by environmental conditions and suggest that β-GlcNAc WTA may bring competitive advantage in vivo.					
30824758	1	77	theme	major	135:139	arg1	formation					336:344	biofilm formation	328:344	biofilm formation	328:344	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	77	theme	major	135:139	arg1	interaction					301:311	host interaction	296:311	host interaction	296:311	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	77	theme	major	135:139	arg1	constituents					141:152	major constituents	135:152	major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology	135:254	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	77	theme	major	135:139	arg1	resistance					257:266	resistance	257:266	resistance to antimicrobial molecules	257:293	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	77	theme	major	135:139	arg1	acid					120:123	Wall teichoic acid	106:123	Wall teichoic acid (WTA)	106:129	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	1	77	theme	major	135:139	arg1	virulence					314:322	virulence	314:322	virulence	314:322	Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation.					
30824758	5	78	from	gene	953:956	arg1	strains					965:971	all strains	961:971	all strains assessed	961:980	A majority of the S. aureus strains produced the β-GlcNAc WTA form in accordance with the presence of the tarS gene in all strains assessed.					
29783688	3	0	theme	present	317:323	arg1	study					325:329	The present study	313:329	The present study	313:329	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	8	1	theme	GAG	1021:1023	arg1	chains					1025:1030	the whelk GAG chains	1011:1030	the whelk GAG chains	1011:1030	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	4	2	with	GAG	494:496	arg1	activity					513:520	cytotoxic activity	503:520	cytotoxic activity on cancer cells	503:536	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	5	3	with	digestion	577:585	arg1	heparinases					592:602	heparinases I, II, and III	592:617	heparinases I, II, and III	592:617	Disaccharides were obtained by sample digestion with heparinases I, II, and III and chondroitinase ABC.					
29783688	8	4	theme	whelk	1015:1019	arg1	chains					1025:1030	the whelk GAG chains	1011:1030	the whelk GAG chains	1011:1030	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	8	5	dep	amounts	901:907	arg1	assessed					942:949	assessed	942:949	assessed by monosaccharides analysis	942:977	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	8	6	theme	chains	1025:1030	arg1	proportion					997:1006	a proportion	995:1006	a proportion of the whelk GAG chains	995:1030	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	11	7	theme	commercial	1502:1511	arg1	GAGs					1523:1526	commercial mammalian GAGs	1502:1526	commercial mammalian GAGs	1502:1526	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	11	8	theme	differences	1467:1477	arg1	evidence					1437:1444	clear evidence	1431:1444	clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs	1431:1526	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	4	9	theme	cancer	525:530	arg1	cells					532:536	cancer cells	525:536	cancer cells	525:536	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	10	10	theme	GAGs	1384:1387	arg1	chains					1389:1394	the anti-cancer GAGs chains	1368:1394	the anti-cancer GAGs chains	1368:1394	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	11	11	theme	mammalian	1513:1521	arg1	GAGs					1523:1526	commercial mammalian GAGs	1502:1526	commercial mammalian GAGs	1502:1526	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	4	12	dep	new	466:468	arg1	undescribed					482:492	undescribed	482:492	undescribed	482:492	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	9	13	theme	disaccharide	1137:1148	arg1	proportions					1111:1121	the proportions	1107:1121	the proportions of individual disaccharide produced in this analysis	1107:1174	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	4	14	from	activity	513:520	arg1	cells					532:536	cancer cells	525:536	cancer cells	525:536	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	6	15	theme	larger	793:798	arg1	size					800:803	their larger size	787:803	their larger size	787:803	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	2	16	theme	cytokines	286:294	arg1	cytokines					286:294	cytokines	286:294	cytokines	286:294	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	16	theme	cytokines	286:294	arg1	variety					259:265	a variety	257:265	a variety of growth factors, cytokines, and chemokines	257:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	16	theme	cytokines	286:294	arg1	factors					277:283	growth factors	270:283	growth factors	270:283	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	16	theme	cytokines	286:294	arg1	chemokines					301:310	chemokines	301:310	chemokines	301:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	7	17	theme	Reduction	855:863	arg1	Labeling					873:880	Glycan Reduction Isotope Labeling	848:880	Glycan Reduction Isotope Labeling (GRIL LCQ-MS)	848:894	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	7	17	theme	Reduction	855:863	arg1	LCQ-MS					888:893	GRIL LCQ-MS	883:893	GRIL LCQ-MS	883:893	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	3	18	theme	key	346:348	arg1	differences					364:374	the key compositional differences	342:374	the key compositional differences between the GAGs isolated from whelk and mammalian GAGs	342:430	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	11	19	theme	clear	1431:1435	arg1	evidence					1437:1444	clear evidence	1431:1444	clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs	1431:1526	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	5	20	theme	chondroitinase	623:636	arg1	ABC					638:640	chondroitinase ABC	623:640	chondroitinase ABC	623:640	Disaccharides were obtained by sample digestion with heparinases I, II, and III and chondroitinase ABC.					
29783688	7	21	theme	Isotope	865:871	arg1	Labeling					873:880	Glycan Reduction Isotope Labeling	848:880	Glycan Reduction Isotope Labeling (GRIL LCQ-MS)	848:894	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	7	21	theme	Isotope	865:871	arg1	LCQ-MS					888:893	GRIL LCQ-MS	883:893	GRIL LCQ-MS	883:893	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	3	22	theme	compositional	350:362	arg1	differences					364:374	the key compositional differences	342:374	the key compositional differences between the GAGs isolated from whelk and mammalian GAGs	342:430	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	4	23	theme	cytotoxic	503:511	arg1	activity					513:520	cytotoxic activity	503:520	cytotoxic activity on cancer cells	503:536	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	0	24	theme	Bioactive	38:46	arg1	Glycosaminoglycans					48:65	Bioactive Glycosaminoglycans	38:65	Bioactive Glycosaminoglycans	38:65	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	2	25	theme	factors	277:283	arg1	cytokines					286:294	cytokines	286:294	cytokines	286:294	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	25	theme	factors	277:283	arg1	variety					259:265	a variety	257:265	a variety of growth factors, cytokines, and chemokines	257:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	25	theme	factors	277:283	arg1	factors					277:283	growth factors	270:283	growth factors	270:283	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	25	theme	factors	277:283	arg1	chemokines					301:310	chemokines	301:310	chemokines	301:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	6	26	theme	due	780:782	arg1	filter					773:778	the filter	769:778	the filter due to their larger size	769:803	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	7	27	theme	Glycan	848:853	arg1	Labeling					873:880	Glycan Reduction Isotope Labeling	848:880	Glycan Reduction Isotope Labeling (GRIL LCQ-MS)	848:894	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	7	27	theme	Glycan	848:853	arg1	LCQ-MS					888:893	GRIL LCQ-MS	883:893	GRIL LCQ-MS	883:893	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	9	28	theme	overall	1204:1210	arg1	proportions					1212:1222	the overall proportions	1200:1222	the overall proportions of disaccharide types within the intact whelk GAGs chain	1200:1279	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	6	29	from	GAGs	685:688	arg1	oligosaccharides					657:672	The resistant oligosaccharides	643:672	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC	643:750	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	6	30	theme	heparinase	703:712	arg1	I					714:714	heparinase I	703:714	heparinase I	703:714	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	2	31	theme	chemokines	301:310	arg1	cytokines					286:294	cytokines	286:294	cytokines	286:294	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	31	theme	chemokines	301:310	arg1	variety					259:265	a variety	257:265	a variety of growth factors, cytokines, and chemokines	257:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	31	theme	chemokines	301:310	arg1	factors					277:283	growth factors	270:283	growth factors	270:283	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	31	theme	chemokines	301:310	arg1	chemokines					301:310	chemokines	301:310	chemokines	301:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	10	32	theme	chains	1389:1394	arg1	make-up					1357:1363	make-up	1357:1363	make-up	1357:1363	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	10	32	theme	chains	1389:1394	arg1	classification					1338:1351	classification	1338:1351	classification	1338:1351	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	0	33	theme	Composition	14:24	arg1	Analysis					26:33	Composition Analysis	14:33	Composition Analysis	14:33	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	4	34	theme	new	466:468	arg1	GAG					494:496	a new, previously undescribed GAG	464:496	a new, previously undescribed GAG with cytotoxic activity on cancer cells	464:536	This polysaccharide represents a new, previously undescribed GAG with cytotoxic activity on cancer cells.					
29783688	7	35	theme	Disaccharide	806:817	arg1	analysis					819:826	Disaccharide analysis	806:826	Disaccharide analysis	806:826	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	2	36	contain	have	201:204	arg2	activity					228:235	remarkable biological activity	206:235	remarkable biological activity	206:235	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	36	contain	have	201:204	arg1	GAGs					196:199	GAGs	196:199	GAGs	196:199	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	36	contain	have	201:204	arg2	co-receptors					240:251	co-receptors	240:251	co-receptors for a variety of growth factors, cytokines, and chemokines	240:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	3	37	theme	mammalian	417:425	arg1	GAGs					427:430	mammalian GAGs	417:430	mammalian GAGs	417:430	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	10	38	dep	classification	1338:1351	arg1	the					1334:1336	the	1334:1336	the	1334:1336	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	8	39	theme	disaccharide	1078:1089	arg1	analysis					1091:1098	the disaccharide analysis	1074:1098	the disaccharide analysis	1074:1098	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	9	40	theme	disaccharide	1227:1238	arg1	types					1240:1244	disaccharide types	1227:1244	disaccharide types	1227:1244	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	11	41	theme	whelk	1487:1491	arg1	GAGs					1493:1496	whelk GAGs	1487:1496	whelk GAGs	1487:1496	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	9	42	theme	types	1240:1244	arg1	proportions					1212:1222	the overall proportions	1200:1222	the overall proportions of disaccharide types within the intact whelk GAGs chain	1200:1279	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	8	43	theme	filter-retained	912:926	arg1	fragments					928:936	filter-retained fragments	912:936	filter-retained fragments	912:936	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	11	44	theme	compositional	1453:1465	arg1	differences					1467:1477	the compositional differences	1449:1477	the compositional differences between whelk GAGs and commercial mammalian GAGs	1449:1526	Furthermore, these data represent clear evidence of the compositional differences between whelk GAGs and commercial mammalian GAGs.					
29783688	0	45	from	Whelk	72:76	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	0	45	from	Whelk	72:76	arg1	Analysis					26:33	Composition Analysis	14:33	Composition Analysis	14:33	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	5	46	theme	sample	570:575	arg1	digestion					577:585	sample digestion	570:585	sample digestion with heparinases I, II, and III	570:617	Disaccharides were obtained by sample digestion with heparinases I, II, and III and chondroitinase ABC.					
29783688	0	47	theme	Glycosaminoglycans	48:65	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	0	47	theme	Glycosaminoglycans	48:65	arg1	Analysis					26:33	Composition Analysis	14:33	Composition Analysis	14:33	Isolation and Composition Analysis of Bioactive Glycosaminoglycans from Whelk.					
29783688	2	48	theme	biological	217:226	arg1	co-receptors					240:251	co-receptors	240:251	co-receptors for a variety of growth factors, cytokines, and chemokines	240:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	48	theme	biological	217:226	arg1	activity					228:235	remarkable biological activity	206:235	remarkable biological activity	206:235	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	3	49	attach	isolated	393:400	arg1	whelk					407:411	whelk	407:411	whelk	407:411	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	3	49	attach	isolated	393:400	arg2	GAGs					388:391	the GAGs	384:391	the GAGs isolated from whelk and mammalian GAGs	384:430	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	3	49	attach	isolated	393:400	arg1	GAGs					427:430	mammalian GAGs	417:430	mammalian GAGs	417:430	The present study identifies the key compositional differences between the GAGs isolated from whelk and mammalian GAGs.					
29783688	6	50	theme	whelk	679:683	arg1	GAGs					685:688	whelk GAGs	679:688	whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC	679:750	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	2	51	theme	remarkable	206:215	arg1	co-receptors					240:251	co-receptors	240:251	co-receptors for a variety of growth factors, cytokines, and chemokines	240:310	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	51	theme	remarkable	206:215	arg1	activity					228:235	remarkable biological activity	206:235	remarkable biological activity	206:235	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	2	52	theme	growth	270:275	arg1	factors					277:283	growth factors	270:283	growth factors	270:283	GAGs have remarkable biological activity as co-receptors for a variety of growth factors, cytokines, and chemokines.					
29783688	8	53	theme	fragments	928:936	arg1	fragments					928:936	filter-retained fragments	912:936	filter-retained fragments	912:936	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	8	53	theme	fragments	928:936	arg1	amounts					901:907	The amounts	897:907	The amounts	897:907	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	10	54	theme	anti-cancer	1372:1382	arg1	chains					1389:1394	the anti-cancer GAGs chains	1368:1394	the anti-cancer GAGs chains	1368:1394	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	9	55	theme	individual	1126:1135	arg1	disaccharide					1137:1148	individual disaccharide	1126:1148	individual disaccharide produced in this analysis	1126:1174	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	9	56	theme	whelk	1264:1268	arg1	chain					1275:1279	the intact whelk GAGs chain	1253:1279	the intact whelk GAGs chain	1253:1279	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	6	57	theme	chondroitinase	733:746	arg1	ABC					748:750	chondroitinase ABC	733:750	chondroitinase ABC	733:750	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	5	58	with	ABC	638:640	arg1	heparinases					592:602	heparinases I, II, and III	592:617	heparinases I, II, and III	592:617	Disaccharides were obtained by sample digestion with heparinases I, II, and III and chondroitinase ABC.					
29783688	10	59	theme	important	1308:1316	arg1	descriptors					1318:1328	important descriptors	1308:1328	important descriptors for the classification and make-up of the anti-cancer GAGs chains	1308:1394	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	10	59	theme	important	1308:1316	arg1	they					1291:1294	they	1291:1294	they	1291:1294	However, they do serve as important descriptors for the classification and make-up of the anti-cancer GAGs chains.					
29783688	9	60	theme	GAGs	1270:1273	arg1	chain					1275:1279	the intact whelk GAGs chain	1253:1279	the intact whelk GAGs chain	1253:1279	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	9	61	theme	intact	1257:1262	arg1	chain					1275:1279	the intact whelk GAGs chain	1253:1279	the intact whelk GAGs chain	1253:1279	Thus, the proportions of individual disaccharide produced in this analysis may not truly represent the overall proportions of disaccharide types within the intact whelk GAGs chain.					
29783688	6	62	theme	resistant	647:655	arg1	oligosaccharides					657:672	The resistant oligosaccharides	643:672	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC	643:750	The resistant oligosaccharides from whelk GAGs treated with heparinase I, II, and III and chondroitinase ABC were retained by the filter due to their larger size.					
29783688	1	63	attach	attached	126:133	arg1	proteins					138:145	proteins	138:145	proteins	138:145	Glycosaminoglycans (GAGs) are found covalently attached to proteins, which create conjugates known as proteoglycans.					
29783688	1	63	attach	attached	126:133	arg2	GAGs					99:102	GAGs	99:102	GAGs	99:102	Glycosaminoglycans (GAGs) are found covalently attached to proteins, which create conjugates known as proteoglycans.					
29783688	1	63	attach	attached	126:133	arg2	Glycosaminoglycans					79:96	Glycosaminoglycans	79:96	Glycosaminoglycans (GAGs)	79:103	Glycosaminoglycans (GAGs) are found covalently attached to proteins, which create conjugates known as proteoglycans.					
29783688	8	64	theme	monosaccharides	954:968	arg1	analysis					970:977	monosaccharides analysis	954:977	monosaccharides analysis	954:977	The amounts of filter-retained fragments, as assessed by monosaccharides analysis, suggested that a proportion of the whelk GAG chains remained resistant to the enzymes used in the disaccharide analysis.					
29783688	1	65	dep	found	109:113	arg1	attached					126:133	attached	126:133	attached to proteins, which create conjugates known as proteoglycans	126:193	Glycosaminoglycans (GAGs) are found covalently attached to proteins, which create conjugates known as proteoglycans.					
29783688	7	66	theme	GRIL	883:886	arg1	Labeling					873:880	Glycan Reduction Isotope Labeling	848:880	Glycan Reduction Isotope Labeling (GRIL LCQ-MS)	848:894	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29783688	7	66	theme	GRIL	883:886	arg1	LCQ-MS					888:893	GRIL LCQ-MS	883:893	GRIL LCQ-MS	883:893	Disaccharide analysis was performed using Glycan Reduction Isotope Labeling (GRIL LCQ-MS).					
29895348	2	0	theme	vera	455:458	arg1	activities					438:447	the biological activities	423:447	the biological activities of A. vera	423:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	1	1	theme	human	295:299	arg1	body					301:304	the human body	291:304	the human body are increasing	291:319	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	6	2	theme	tissue	1253:1258	arg1	screening					1220:1228	high-throughput screening	1204:1228	high-throughput screening of aloe leaf mesophyll tissue	1204:1258	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	5	3	dep	Methods	926:932	arg1	method					939:944	The method	935:944	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.	926:1125	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	2	4	theme	A.	452:453	arg1	vera					455:458	A. vera	452:458	A. vera	452:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	3	5	theme	polysaccharide	627:640	arg1	compositions					642:653	polysaccharide compositions	627:653	polysaccharide compositions	627:653	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	4	6	theme	carbohydrate	801:812	arg1	microarrays					814:824	carbohydrate microarrays	801:824	carbohydrate microarrays	801:824	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	8	7	theme	Aloe	1537:1540	arg1	species					1526:1532	true species	1521:1532	true species of Aloe	1521:1540	Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.					
29895348	3	8	theme	compositions	642:653	arg1	studies					616:622	large comparative studies	598:622	large comparative studies of polysaccharide compositions	598:653	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	6	9	theme	leaf	1238:1241	arg1	tissue					1253:1258	aloe leaf mesophyll tissue	1233:1258	aloe leaf mesophyll tissue	1233:1258	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	6	10	theme	Preliminary	1136:1146	arg1	findings					1148:1155	Preliminary findings	1136:1155	Preliminary findings	1136:1155	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	6	11	used	used	1195:1198	arg2	CoMPP					1169:1173	CoMPP	1169:1173	CoMPP	1169:1173	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	4	12	used	used	835:838	arg2	microarrays					814:824	carbohydrate microarrays	801:824	carbohydrate microarrays	801:824	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	4	13	theme	polysaccharides	886:900	arg1	analysis					864:871	the high-throughput analysis	844:871	the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll	844:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	6	14	theme	aloe	1233:1236	arg1	tissue					1253:1258	aloe leaf mesophyll tissue	1233:1258	aloe leaf mesophyll tissue	1233:1258	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	7	15	theme	tissues	1404:1410	arg1	compositions					1374:1385	the polysaccharide compositions	1355:1385	the polysaccharide compositions of the mesophyll tissues	1355:1410	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	4	16	from	polysaccharides	886:900	arg1	mesophyll					915:923	aloe leaf mesophyll	905:923	aloe leaf mesophyll	905:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	4	17	theme	leaf	910:913	arg1	mesophyll					915:923	aloe leaf mesophyll	905:923	aloe leaf mesophyll	905:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	5	18	theme	high-throughput	1035:1049	arg1	capacity					1051:1058	the high-throughput capacity	1031:1058	the high-throughput capacity of microarray technology with the specificity of molecular probes	1031:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	4	19	theme	aloe	905:908	arg1	mesophyll					915:923	aloe leaf mesophyll	905:923	aloe leaf mesophyll	905:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	1	20	dep	effects	270:276	arg1	they					278:281	they	278:281	they	278:281	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	2	21	theme	leaf	353:356	arg1	mesophyll					358:366	the leaf mesophyll	349:366	the leaf mesophyll	349:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	6	22	theme	high-throughput	1204:1218	arg1	screening					1220:1228	high-throughput screening	1204:1228	high-throughput screening of aloe leaf mesophyll tissue	1204:1258	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	8	23	theme	true	1521:1524	arg1	species					1526:1532	true species	1521:1532	true species of Aloe	1521:1540	Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.					
29895348	7	24	theme	polysaccharide	1359:1372	arg1	compositions					1374:1385	the polysaccharide compositions	1355:1385	the polysaccharide compositions of the mesophyll tissues	1355:1410	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	7	25	theme	Aloe	1282:1285	arg1	species					1271:1277	Seventeen species	1261:1277	Seventeen species of Aloe	1261:1285	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	7	25	theme	Aloe	1282:1285	arg1	genera					1307:1312	closely related genera	1291:1312	closely related genera	1291:1312	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	2	26	theme	responsible	407:417	arg1	Polysaccharides					322:336	Polysaccharides	322:336	Polysaccharides present in the leaf mesophyll	322:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	2	26	theme	responsible	407:417	arg1	components					396:405	the components	392:405	the components responsible for the biological activities of A. vera	392:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	1	27	dep	body	301:304	arg1	increasing					310:319	increasing	310:319	are increasing	306:319	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	8	28	theme	preliminary	1441:1451	arg1	data					1453:1456	These preliminary data	1435:1456	These preliminary data	1435:1456	Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.					
29895348	2	29	from	present	338:344	arg1	mesophyll					358:366	the leaf mesophyll	349:366	the leaf mesophyll	349:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	6	30	theme	mesophyll	1243:1251	arg1	tissue					1253:1258	aloe leaf mesophyll tissue	1233:1258	aloe leaf mesophyll tissue	1233:1258	Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue.					
29895348	0	31	theme	Polysaccharides	17:31	arg1	Analyses					0:7	Analyses	0:7	Analyses of Aloe Polysaccharides	0:31	Analyses of Aloe Polysaccharides Using Carbohydrate Microarray Profiling.					
29895348	3	32	theme	near-complete	694:706	arg1	fractionation					708:720	a complete or near-complete fractionation	680:720	a complete or near-complete fractionation of the polymers	680:736	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	2	33	theme	past	502:505	arg1	decades					507:513	the past decades	498:513	the past decades	498:513	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	5	34	theme	comprehensive	967:979	arg1	profiling					1000:1008	comprehensive microarray polymer profiling	967:1008	comprehensive microarray polymer profiling (CoMPP)	967:1016	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	34	theme	comprehensive	967:979	arg1	CoMPP					1011:1015	CoMPP	1011:1015	CoMPP	1011:1015	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	7	35	theme	clear	1335:1339	arg1	difference					1341:1350	a clear difference	1333:1350	a clear difference in the polysaccharide compositions of the mesophyll tissues	1333:1410	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	1	36	theme	individual	178:187	arg1	components					197:206	the individual polymer components	174:206	the individual polymer components of the leaf mesophyll	174:228	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	0	37	theme	Aloe	12:15	arg1	Polysaccharides					17:31	Aloe Polysaccharides	12:31	Aloe Polysaccharides	12:31	Analyses of Aloe Polysaccharides Using Carbohydrate Microarray Profiling.					
29895348	5	38	theme	technology	1074:1083	arg1	capacity					1051:1058	the high-throughput capacity	1031:1058	the high-throughput capacity of microarray technology with the specificity of molecular probes	1031:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	1	39	theme	Aloe	107:110	arg1	extracts					117:124	Aloe vera extracts	107:124	Aloe vera extracts	107:124	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	1	40	theme	polymer	189:195	arg1	components					197:206	the individual polymer components	174:206	the individual polymer components of the leaf mesophyll	174:228	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	0	41	theme	Carbohydrate	39:50	arg1	Profiling					63:71	Carbohydrate Microarray Profiling	39:71	Carbohydrate Microarray Profiling	39:71	Analyses of Aloe Polysaccharides Using Carbohydrate Microarray Profiling.					
29895348	3	42	theme	complete	682:689	arg1	fractionation					708:720	a complete or near-complete fractionation	680:720	a complete or near-complete fractionation of the polymers	680:736	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	4	43	theme	wall	881:884	arg1	polysaccharides					886:900	cell wall polysaccharides	876:900	cell wall polysaccharides in aloe leaf mesophyll	876:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	3	44	theme	used	538:541	arg1	methods					543:549	the commonly used methods	525:549	the commonly used methods	525:549	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	7	45	from	difference	1341:1350	arg1	compositions					1374:1385	the polysaccharide compositions	1355:1385	the polysaccharide compositions of the mesophyll tissues	1355:1410	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	4	46	theme	cell	876:879	arg1	polysaccharides					886:900	cell wall polysaccharides	876:900	cell wall polysaccharides in aloe leaf mesophyll	876:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	3	47	theme	desired	570:576	arg1	platform					578:585	the desired platform	566:585	the desired platform to conduct large comparative studies of polysaccharide compositions	566:653	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	5	48	dep	method	939:944	arg1	chose					949:953	chose	949:953	chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes	949:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	2	49	theme	biological	427:436	arg1	activities					438:447	the biological activities	423:447	the biological activities of A. vera	423:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	3	50	theme	polymers	729:736	arg1	fractionation					708:720	a complete or near-complete fractionation	680:720	a complete or near-complete fractionation of the polymers	680:736	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	4	51	from	analysis	864:871	arg1	mesophyll					915:923	aloe leaf mesophyll	905:923	aloe leaf mesophyll	905:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	8	52	dep	genera	1568:1573	arg1	segregate					1558:1566	segregate	1558:1566	segregate	1558:1566	Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.					
29895348	1	53	theme	vera	112:115	arg1	extracts					117:124	Aloe vera extracts	107:124	Aloe vera extracts	107:124	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	0	54	theme	Microarray	52:61	arg1	Profiling					63:71	Carbohydrate Microarray Profiling	39:71	Carbohydrate Microarray Profiling	39:71	Analyses of Aloe Polysaccharides Using Carbohydrate Microarray Profiling.					
29895348	5	55	theme	molecular	1109:1117	arg1	probes					1119:1124	molecular probes	1109:1124	molecular probes	1109:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	56	theme	microarray	981:990	arg1	profiling					1000:1008	comprehensive microarray polymer profiling	967:1008	comprehensive microarray polymer profiling (CoMPP)	967:1016	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	56	theme	microarray	981:990	arg1	CoMPP					1011:1015	CoMPP	1011:1015	CoMPP	1011:1015	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	57	theme	polymer	992:998	arg1	profiling					1000:1008	comprehensive microarray polymer profiling	967:1008	comprehensive microarray polymer profiling (CoMPP)	967:1016	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	57	theme	polymer	992:998	arg1	CoMPP					1011:1015	CoMPP	1011:1015	CoMPP	1011:1015	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	2	58	from	mesophyll	358:366	arg1	present					338:344	present	338:344	present	338:344	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	1	59	theme	extracts	117:124	arg1	popularity					93:102	the popularity	89:102	the popularity of Aloe vera extracts	89:124	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	5	60	with	capacity	1051:1058	arg1	specificity					1094:1104	the specificity	1090:1104	the specificity of molecular probes	1090:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	5	61	theme	probes	1119:1124	arg1	specificity					1094:1104	the specificity	1090:1104	the specificity of molecular probes	1090:1124	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	4	62	theme	high-throughput	848:862	arg1	analysis					864:871	the high-throughput analysis	844:871	the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll	844:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
29895348	1	63	dep	one	249:251	arg1	another					253:259	another	253:259	another	253:259	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	3	64	theme	large	598:602	arg1	studies					616:622	large comparative studies	598:622	large comparative studies of polysaccharide compositions	598:653	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	7	65	theme	mesophyll	1394:1402	arg1	tissues					1404:1410	the mesophyll tissues	1390:1410	the mesophyll tissues	1390:1410	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	1	66	theme	leaf	215:218	arg1	mesophyll					220:228	the leaf mesophyll	211:228	the leaf mesophyll	211:228	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	3	67	theme	comparative	604:614	arg1	studies					616:622	large comparative studies	598:622	large comparative studies of polysaccharide compositions	598:653	However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.					
29895348	2	68	attach	present	338:344	arg2	Polysaccharides					322:336	Polysaccharides	322:336	Polysaccharides present in the leaf mesophyll	322:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	2	68	attach	present	338:344	arg1	mesophyll					358:366	the leaf mesophyll	349:366	the leaf mesophyll	349:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	2	68	attach	present	338:344	arg2	components					396:405	the components	392:405	the components responsible for the biological activities of A. vera	392:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	2	69	theme	present	338:344	arg1	Polysaccharides					322:336	Polysaccharides	322:336	Polysaccharides present in the leaf mesophyll	322:366	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	2	69	theme	present	338:344	arg1	components					396:405	the components	392:405	the components responsible for the biological activities of A. vera	392:458	Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades.					
29895348	7	70	theme	related	1299:1305	arg1	genera					1307:1312	closely related genera	1291:1312	closely related genera	1291:1312	Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.					
29895348	1	71	theme	mesophyll	220:228	arg1	components					197:206	the individual polymer components	174:206	the individual polymer components of the leaf mesophyll	174:228	Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing.					
29895348	5	72	theme	microarray	1063:1072	arg1	technology					1074:1083	microarray technology	1063:1083	microarray technology	1063:1083	Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.					
29895348	4	73	from	mesophyll	915:923	arg1	analysis					864:871	the high-throughput analysis	844:871	the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll	844:923	Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.					
31351958	0	0	theme	nanoparticles	87:99	arg1	Effect					71:76	Effect	71:76	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.	0:164	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	5	1	theme	BC	866:867	arg1	yield					869:873	the BC yield	862:873	the BC yield	862:873	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	0	2	theme	glass	81:85	arg1	nanoparticles					87:99	glass nanoparticles	81:99	glass nanoparticles	81:99	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	3	3	theme	culture	648:654	arg1	medium					656:661	BC producing culture medium	635:661	BC producing culture medium	635:661	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	7	4	theme	bone	1201:1204	arg1	regeneration					1206:1217	bone regeneration	1201:1217	bone regeneration	1201:1217	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	5	5	from	NBG	923:925	arg1	value					937:941	the pH value	930:941	the pH value of the culture medium	930:963	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	0	6	theme	cellulose	104:112	arg1	yield					114:118	cellulose yield	104:118	cellulose yield	104:118	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	7	7	with	regeneration	1206:1217	arg1	unique					1246:1251	unique	1246:1251	unique	1246:1251	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	4	8	theme	cellulose	720:728	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	1	9	theme	little	263:268	arg1	bioactivity					270:280	little bioactivity	263:280	little bioactivity	263:280	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	7	10	theme	wound	1223:1227	arg1	healing					1229:1235	wound healing	1223:1235	wound healing	1223:1235	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	4	11	theme	biocompatibility	731:746	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	4	12	theme	antimicrobial	765:777	arg1	activity					779:786	antimicrobial activity	765:786	antimicrobial activity	765:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	5	13	theme	pH	934:935	arg1	value					937:941	the pH value	930:941	the pH value of the culture medium	930:963	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	1	14	theme	bacterial	192:200	arg1	cellulose					202:210	bacterial cellulose	192:210	bacterial cellulose (BC)	192:215	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	1	14	theme	bacterial	192:200	arg1	BC					213:214	BC	213:214	BC	213:214	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	7	15	theme	biomedical	1171:1180	arg1	regeneration					1206:1217	bone regeneration	1201:1217	bone regeneration	1201:1217	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	7	15	theme	biomedical	1171:1180	arg1	healing					1229:1235	wound healing	1223:1235	wound healing	1223:1235	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	7	15	theme	biomedical	1171:1180	arg1	industries					1182:1191	biomedical industries	1171:1191	biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans	1171:1282	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	1	16	theme	cellulose	202:210	arg1	advantages					178:187	the advantages	174:187	the advantages of bacterial cellulose (BC) over traditional cellulose	174:242	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	0	17	theme	Green	0:4	arg1	synthesis					6:14	Green synthesis	0:14	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.	0:164	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	7	18	theme	BC/NBG	1114:1119	arg1	composite					1121:1129	BC/NBG composite	1114:1129	BC/NBG composite	1114:1129	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	0	19	theme	bacterial	19:27	arg1	nanocomposites					55:68	bacterial cellulose/bioactive glass nanocomposites	19:68	bacterial cellulose/bioactive glass nanocomposites	19:68	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	3	20	theme	in	566:567	arg1	approach					587:594	in situ fermentation approach	566:594	in situ fermentation approach	566:594	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	3	21	dep	in	566:567	arg1	situ					569:572	situ	569:572	situ	569:572	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	2	22	theme	nanobioactive	402:414	arg1	NBG					423:425	NBG	423:425	NBG	423:425	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	2	22	theme	nanobioactive	402:414	arg1	glass					416:420	a nanobioactive glass	400:420	a nanobioactive glass (NBG)	400:426	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	2	23	theme	green	506:510	arg1	method					512:517	a novel and simple green method	487:517	a novel and simple green method	487:517	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	4	24	theme	production	698:707	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	0	25	theme	glass	49:53	arg1	nanocomposites					55:68	bacterial cellulose/bioactive glass nanocomposites	19:68	bacterial cellulose/bioactive glass nanocomposites	19:68	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	2	26	theme	simple	499:504	arg1	method					512:517	a novel and simple green method	487:517	a novel and simple green method	487:517	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	3	27	theme	NBG	616:618	arg1	particles					620:628	NBG particles	616:628	NBG particles	616:628	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	5	28	theme	medium	958:963	arg1	value					937:941	the pH value	930:941	the pH value of the culture medium	930:963	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	0	29	theme	cellulose/bioactive	29:47	arg1	nanocomposites					55:68	bacterial cellulose/bioactive glass nanocomposites	19:68	bacterial cellulose/bioactive glass nanocomposites	19:68	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	0	30	theme	antimicrobial	142:154	arg1	activity					156:163	antimicrobial activity	142:163	antimicrobial activity	142:163	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	4	31	theme	activity	779:786	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	1	32	theme	traditional	222:232	arg1	cellulose					234:242	traditional cellulose	222:242	traditional cellulose	222:242	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	2	33	theme	novel	489:493	arg1	method					512:517	a novel and simple green method	487:517	a novel and simple green method	487:517	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	0	34	from	Effect	71:76	arg1	biocompatibility					121:136	biocompatibility	121:136	biocompatibility	121:136	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	0	34	from	Effect	71:76	arg1	activity					156:163	antimicrobial activity	142:163	antimicrobial activity	142:163	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	0	34	from	Effect	71:76	arg1	yield					114:118	cellulose yield	104:118	cellulose yield	104:118	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	6	35	theme	BC	1084:1085	arg1	biocompatibility					1035:1050	biocompatibility	1035:1050	biocompatibility	1035:1050	Moreover, it was effectively improved biocompatibility and antimicrobial properties of BC.					
31351958	6	35	theme	BC	1084:1085	arg1	properties					1070:1079	antimicrobial properties	1056:1079	antimicrobial properties	1056:1079	Moreover, it was effectively improved biocompatibility and antimicrobial properties of BC.					
31351958	0	36	theme	nanocomposites	55:68	arg1	synthesis					6:14	Green synthesis	0:14	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.	0:164	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	3	37	theme	BC	635:636	arg1	medium					656:661	BC producing culture medium	635:661	BC producing culture medium	635:661	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	5	38	theme	fermentation	976:987	arg1	period					989:994	the fermentation period	972:994	the fermentation period	972:994	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	3	39	theme	producing	638:646	arg1	medium					656:661	BC producing culture medium	635:661	BC producing culture medium	635:661	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	3	40	theme	fermentation	574:585	arg1	approach					587:594	in situ fermentation approach	566:594	in situ fermentation approach	566:594	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	4	41	theme	addition	682:689	arg1	effect					668:673	The effect	664:673	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	664:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	3	42	theme	particles	620:628	arg1	incorporation					599:611	incorporation	599:611	incorporation of NBG particles into BC producing culture medium	599:661	Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium.					
31351958	4	43	theme	NBG	678:680	arg1	addition					682:689	NBG addition	678:689	NBG addition	678:689	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	4	44	theme	bioactivity	749:759	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	2	45	theme	bioactive	459:467	arg1	nanocomposites					469:482	BC/NBG bioactive nanocomposites	452:482	BC/NBG bioactive nanocomposites	452:482	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	2	46	theme	BC	385:386	arg1	yield					388:392	the BC yield	381:392	the BC yield using a nanobioactive glass (NBG)	381:426	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	0	47	dep	synthesis	6:14	arg1	Effect					71:76	Effect	71:76	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.	0:164	Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity.					
31351958	7	48	with	healing	1229:1235	arg1	unique					1246:1251	unique	1246:1251	unique	1246:1251	This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.					
31351958	4	49	from	effect	668:673	arg1	process					709:715	the production process	694:715	the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity	694:786	The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated.					
31351958	2	50	theme	BC/NBG	452:457	arg1	nanocomposites					469:482	BC/NBG bioactive nanocomposites	452:482	BC/NBG bioactive nanocomposites	452:482	This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method.					
31351958	1	51	theme	industrial	318:327	arg1	scale					329:333	an industrial scale	315:333	an industrial scale	315:333	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
31351958	5	52	theme	culture	950:956	arg1	medium					958:963	the culture medium	946:963	the culture medium	946:963	The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period.					
31351958	6	53	theme	antimicrobial	1056:1068	arg1	properties					1070:1079	antimicrobial properties	1056:1079	antimicrobial properties	1056:1079	Moreover, it was effectively improved biocompatibility and antimicrobial properties of BC.					
31351958	1	54	theme	low	249:251	arg1	yield					253:257	low yield	249:257	low yield	249:257	Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale.					
30003921	8	0	theme	carbohydrate	1042:1053	arg1	assay					1067:1071	the limited carbohydrate competition assay	1030:1071	the limited carbohydrate competition assay	1030:1071	In the limited carbohydrate competition assay, arabinose, fucose and fetuin inhibited BinA toxicity towards Culex larvae.					
30003921	9	1	theme	different	1260:1268	arg1	proteins					1283:1290	diverse and structurally different glycosylated proteins	1235:1290	diverse and structurally different glycosylated proteins	1235:1290	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	12	2	theme	polypeptide	1834:1844	arg1	core					1802:1805	glycan core	1795:1805	glycan core of GPI anchor and receptor polypeptide	1795:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	5	3	theme	high-throughput	721:735	arg1	screening					737:745	initial high-throughput screening	713:745	initial high-throughput screening	713:745	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	2	4	theme	BinAB	308:312	arg1	structure					295:303	Recently reported crystal structure	269:303	Recently reported crystal structure of BinAB	269:312	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	9	5	gly	glycosylated	1270:1281	arg1	proteins					1283:1290	diverse and structurally different glycosylated proteins	1235:1290	diverse and structurally different glycosylated proteins	1235:1290	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	12	6	theme	GPI	1810:1812	arg1	anchor					1814:1819	GPI anchor	1810:1819	GPI anchor	1810:1819	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	2	7	theme	lectin-like	350:360	arg1	domain					362:367	N-terminal lectin-like domain	339:367	N-terminal lectin-like domain	339:367	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	5	8	gly	glycoproteins	684:696	arg1	glycoproteins					684:696	glycoproteins	684:696	glycoproteins	684:696	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	1	9	theme	several	231:237	arg1	species					245:251	several Aedes species	231:251	several Aedes species	231:251	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	12	10	theme	toxin-receptor	1702:1715	arg1	specificity					1717:1727	toxin-receptor specificity	1702:1727	toxin-receptor specificity of BinA in Culex	1702:1744	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	10	11	theme	intracellular	1328:1340	arg1	cytotoxicity					1342:1353	its intracellular cytotoxicity	1324:1353	its intracellular cytotoxicity	1324:1353	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	11	12	gly	glycoproteins	1460:1472	arg1	glycoproteins					1460:1472	The glycoproteins	1456:1472	The glycoproteins	1456:1472	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	12	13	theme	receptor	1660:1667	arg1	polypeptide					1669:1679	receptor polypeptide	1660:1679	receptor polypeptide	1660:1679	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	1	14	theme	BinAB	148:152	arg1	toxin					154:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	12	15	theme	glycan	1795:1800	arg1	core					1802:1805	glycan core	1795:1805	glycan core of GPI anchor and receptor polypeptide	1795:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	10	16	gly	N-glycosylation	1367:1381	arg1	larvae					1448:1453	insect larvae	1441:1453	insect larvae	1441:1453	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	12	17	with	interaction	1770:1780	arg1	core					1802:1805	glycan core	1795:1805	glycan core of GPI anchor and receptor polypeptide	1795:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	2	18	from	presence	327:334	arg1	BinA					372:375	BinA	372:375	BinA	372:375	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	1	19	dep	Lysinibacillus	111:124	arg1	sphaericus					126:135	sphaericus	126:135	sphaericus	126:135	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	11	20	theme	pull-down	1542:1550	arg1	assay					1552:1556	the pull-down assay	1538:1556	the pull-down assay using affinity immobilized BinA	1538:1588	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	2	21	theme	reported	278:285	arg1	structure					295:303	Recently reported crystal structure	269:303	Recently reported crystal structure of BinAB	269:312	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	1	22	theme	larvicidal	178:187	arg1	activity					189:196	mosquito larvicidal activity	169:196	mosquito larvicidal activity against Culex and Anopheles	169:224	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	13	23	theme	toxin	1978:1982	arg1	specificity					1957:1967	target specificity	1950:1967	target specificity of BinAB toxin	1950:1982	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	0	24	theme	BinA	83:86	arg1	cytotoxicity					97:108	BinA mediated cytotoxicity	83:108	BinA mediated cytotoxicity	83:108	Mosquito-larvicidal BinA toxin displays affinity for glycoconjugates: Proposal for BinA mediated cytotoxicity.					
30003921	8	25	theme	BinA	1113:1116	arg1	toxicity					1118:1125	BinA toxicity	1113:1125	BinA toxicity towards Culex larvae	1113:1146	In the limited carbohydrate competition assay, arabinose, fucose and fetuin inhibited BinA toxicity towards Culex larvae.					
30003921	12	26	theme	BinA	1785:1788	arg1	interaction					1770:1780	dual interaction	1765:1780	dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide	1765:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	4	27	theme	BinA	528:531	arg1	specificity					513:523	carbohydrate specificity	500:523	carbohydrate specificity of BinA	500:531	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	13	28	theme	target	1950:1955	arg1	specificity					1957:1967	target specificity	1950:1967	target specificity of BinAB toxin	1950:1982	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	5	29	theme	saccharides	635:645	arg1	total					623:627	A total	621:627	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins)	621:697	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	4	30	theme	high-throughput	536:550	arg1	approaches					552:561	high-throughput approaches	536:561	high-throughput approaches using extrinsic fluorescence and thermofluor shift assay	536:618	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	0	31	theme	Mosquito-larvicidal	0:18	arg1	toxin					25:29	Mosquito-larvicidal BinA toxin	0:29	Mosquito-larvicidal BinA toxin	0:29	Mosquito-larvicidal BinA toxin displays affinity for glycoconjugates: Proposal for BinA mediated cytotoxicity.					
30003921	10	32	theme	protein	1359:1365	arg1	N-glycosylation					1367:1381	protein N-glycosylation	1359:1381	protein N-glycosylation	1359:1381	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	12	33	theme	BinA	1732:1735	arg1	specificity					1717:1727	toxin-receptor specificity	1702:1727	toxin-receptor specificity of BinA in Culex	1702:1744	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	9	34	theme	direct	1188:1193	arg1	evidence					1195:1202	direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins	1188:1290	direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins	1188:1290	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	7	35	theme	differential	880:891	arg1	binding					893:899	differential binding	880:899	differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin)	880:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	3	36	theme	present	454:460	arg1	studies					462:468	the present studies	450:468	the present studies	450:468	Hemagglutination and hemolytic activities were not observed for BinA in the present studies.					
30003921	11	37	with	complexes	1493:1501	arg1	BinA					1508:1511	BinA	1508:1511	BinA	1508:1511	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	11	38	theme	affinity	1564:1571	arg1	BinA					1585:1588	affinity immobilized BinA	1564:1588	affinity immobilized BinA	1564:1588	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	13	39	dep	strategies	1886:1895	arg1	broadening					1939:1948	broadening	1939:1948	broadening target specificity of BinAB toxin	1939:1982	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	13	39	dep	strategies	1886:1895	arg1	improving					1901:1909	improving	1901:1909	improving larvicidal activity	1901:1929	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	0	40	dep	glycoconjugates	53:67	arg1	Proposal					70:77	Proposal	70:77	glycoconjugates: Proposal	53:77	Mosquito-larvicidal BinA toxin displays affinity for glycoconjugates: Proposal for BinA mediated cytotoxicity.					
30003921	4	41	theme	thermofluor	596:606	arg1	assay					614:618	extrinsic fluorescence and thermofluor shift assay	569:618	assay	614:618	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	7	42	theme	Surface	845:851	arg1	resonance					861:869	Surface plasmon resonance	845:869	Surface plasmon resonance	845:869	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	3	43	located	observed	429:436	arg2	activities					409:418	Hemagglutination and hemolytic activities	378:418	activities	409:418	Hemagglutination and hemolytic activities were not observed for BinA in the present studies.					
30003921	3	43	located	observed	429:436	arg1	studies					462:468	the present studies	450:468	the present studies	450:468	Hemagglutination and hemolytic activities were not observed for BinA in the present studies.					
30003921	8	44	theme	limited	1034:1040	arg1	assay					1067:1071	the limited carbohydrate competition assay	1030:1071	the limited carbohydrate competition assay	1030:1071	In the limited carbohydrate competition assay, arabinose, fucose and fetuin inhibited BinA toxicity towards Culex larvae.					
30003921	9	45	theme	diverse	1235:1241	arg1	proteins					1283:1290	diverse and structurally different glycosylated proteins	1235:1290	diverse and structurally different glycosylated proteins	1235:1290	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	8	46	theme	competition	1055:1065	arg1	assay					1067:1071	the limited carbohydrate competition assay	1030:1071	the limited carbohydrate competition assay	1030:1071	In the limited carbohydrate competition assay, arabinose, fucose and fetuin inhibited BinA toxicity towards Culex larvae.					
30003921	10	47	theme	larvae	1448:1453	arg1	survival					1429:1436	survival	1429:1436	survival	1429:1436	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	10	47	theme	larvae	1448:1453	arg1	development					1413:1423	development	1413:1423	development	1413:1423	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	13	48	theme	BinAB	1972:1976	arg1	toxin					1978:1982	BinAB toxin	1972:1982	BinAB toxin	1972:1982	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	7	49	with	affinity	973:980	arg1	asialofetuin					937:948	asialofetuin	937:948	asialofetuin	937:948	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	49	with	affinity	973:980	arg1	fetuin					929:934	fetuin	929:934	fetuin	929:934	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	49	with	affinity	973:980	arg1	glycoproteins					914:926	glycoproteins	914:926	glycoproteins (fetuin, asialofetuin and thyroglobulin)	914:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	49	with	affinity	973:980	arg1	thyroglobulin					954:966	thyroglobulin	954:966	thyroglobulin	954:966	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	6	50	from	change	807:812	arg1	intensity					834:842	the fluorescence intensity	817:842	the fluorescence intensity	817:842	The promising glycans were identified based on significant change in the fluorescence intensity.					
30003921	5	51	dep	saccharides	635:645	arg1	mono-					648:652	mono-	648:652	mono-	648:652	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	51	dep	saccharides	635:645	arg1	saccharides					635:645	34 saccharides	632:645	34 saccharides (mono-, di- and polysaccharides, and glycoproteins)	632:697	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	51	dep	saccharides	635:645	arg1	polysaccharides					663:677	polysaccharides	663:677	polysaccharides	663:677	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	51	dep	saccharides	635:645	arg1	di-					655:657	di-	655:657	di-	655:657	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	52	theme	initial	713:719	arg1	screening					737:745	initial high-throughput screening	713:745	initial high-throughput screening	713:745	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	9	53	theme	glycosylated	1270:1281	arg1	proteins					1283:1290	diverse and structurally different glycosylated proteins	1235:1290	diverse and structurally different glycosylated proteins	1235:1290	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	5	54	used	used	704:707	arg2	total					623:627	A total	621:627	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins)	621:697	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	12	55	theme	anchor	1814:1819	arg1	core					1802:1805	glycan core	1795:1805	glycan core of GPI anchor and receptor polypeptide	1795:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	7	56	with	binding	893:899	arg1	asialofetuin					937:948	asialofetuin	937:948	asialofetuin	937:948	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	56	with	binding	893:899	arg1	fetuin					929:934	fetuin	929:934	fetuin	929:934	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	56	with	binding	893:899	arg1	glycoproteins					914:926	glycoproteins	914:926	glycoproteins (fetuin, asialofetuin and thyroglobulin)	914:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	56	with	binding	893:899	arg1	thyroglobulin					954:966	thyroglobulin	954:966	thyroglobulin	954:966	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	2	57	theme	domain	362:367	arg1	presence					327:334	the presence	323:334	the presence of N-terminal lectin-like domain in BinA	323:375	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	12	58	theme	receptor	1825:1832	arg1	polypeptide					1834:1844	receptor polypeptide	1825:1844	receptor polypeptide	1825:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	2	59	theme	N-terminal	339:348	arg1	domain					362:367	N-terminal lectin-like domain	339:367	N-terminal lectin-like domain	339:367	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	12	60	with	affinity	1646:1653	arg1	polypeptide					1669:1679	receptor polypeptide	1660:1679	receptor polypeptide	1660:1679	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	4	61	theme	carbohydrate	500:511	arg1	specificity					513:523	carbohydrate specificity	500:523	carbohydrate specificity of BinA	500:531	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	1	62	theme	Aedes	239:243	arg1	species					245:251	several Aedes species	231:251	several Aedes species	231:251	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	1	63	theme	parasporal	137:146	arg1	toxin					154:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	11	64	theme	immobilized	1573:1583	arg1	BinA					1585:1588	affinity immobilized BinA	1564:1588	affinity immobilized BinA	1564:1588	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	5	65	theme	34	632:633	arg1	mono-					648:652	mono-	648:652	mono-	648:652	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	65	theme	34	632:633	arg1	saccharides					635:645	34 saccharides	632:645	34 saccharides (mono-, di- and polysaccharides, and glycoproteins)	632:697	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	5	65	theme	34	632:633	arg1	di-					655:657	di-	655:657	di-	655:657	A total of 34 saccharides (mono-, di- and polysaccharides, and glycoproteins) were used for initial high-throughput screening.					
30003921	12	66	theme	mild	1641:1644	arg1	affinity					1646:1653	only mild affinity	1636:1653	only mild affinity with receptor polypeptide	1636:1679	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	2	67	theme	crystal	287:293	arg1	structure					295:303	Recently reported crystal structure	269:303	Recently reported crystal structure of BinAB	269:312	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	1	68	theme	mosquito	169:176	arg1	activity					189:196	mosquito larvicidal activity	169:196	mosquito larvicidal activity against Culex and Anopheles	169:224	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	3	69	theme	Hemagglutination	378:393	arg1	activities					409:418	Hemagglutination and hemolytic activities	378:418	activities	409:418	Hemagglutination and hemolytic activities were not observed for BinA in the present studies.					
30003921	4	70	theme	extrinsic	569:577	arg1	fluorescence					579:590	extrinsic fluorescence and thermofluor shift assay	569:618	fluorescence	579:590	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	11	71	theme	native-PAGE	1597:1607	arg1	analysis					1609:1616	native-PAGE analysis	1597:1616	native-PAGE analysis	1597:1616	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	0	72	theme	mediated	88:95	arg1	cytotoxicity					97:108	BinA mediated cytotoxicity	83:108	BinA mediated cytotoxicity	83:108	Mosquito-larvicidal BinA toxin displays affinity for glycoconjugates: Proposal for BinA mediated cytotoxicity.					
30003921	12	73	theme	dual	1765:1768	arg1	interaction					1770:1780	dual interaction	1765:1780	dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide	1765:1844	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	7	74	gly	glycoproteins	914:926	arg1	asialofetuin					937:948	asialofetuin	937:948	asialofetuin	937:948	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	74	gly	glycoproteins	914:926	arg1	fetuin					929:934	fetuin	929:934	fetuin	929:934	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	74	gly	glycoproteins	914:926	arg1	glycoproteins					914:926	glycoproteins	914:926	glycoproteins (fetuin, asialofetuin and thyroglobulin)	914:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	74	gly	glycoproteins	914:926	arg1	thyroglobulin					954:966	thyroglobulin	954:966	thyroglobulin	954:966	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	8	75	theme	Culex	1135:1139	arg1	larvae					1141:1146	Culex larvae	1135:1146	Culex larvae	1135:1146	In the limited carbohydrate competition assay, arabinose, fucose and fetuin inhibited BinA toxicity towards Culex larvae.					
30003921	3	76	theme	hemolytic	399:407	arg1	activities					409:418	Hemagglutination and hemolytic activities	378:418	activities	409:418	Hemagglutination and hemolytic activities were not observed for BinA in the present studies.					
30003921	9	77	theme	first	1168:1172	arg1	time					1174:1177	the first time	1164:1177	the first time	1164:1177	This study for the first time provides direct evidence that BinA is competent to bind diverse and structurally different glycosylated proteins.					
30003921	0	78	theme	BinA	20:23	arg1	toxin					25:29	Mosquito-larvicidal BinA toxin	0:29	Mosquito-larvicidal BinA toxin	0:29	Mosquito-larvicidal BinA toxin displays affinity for glycoconjugates: Proposal for BinA mediated cytotoxicity.					
30003921	6	79	theme	promising	752:760	arg1	glycans					762:768	The promising glycans	748:768	The promising glycans	748:768	The promising glycans were identified based on significant change in the fluorescence intensity.					
30003921	7	80	theme	BinA	904:907	arg1	affinity					973:980	affinity	973:980	affinity for simple sugars, l-fucose and l-arabinose	973:1024	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	80	theme	BinA	904:907	arg1	binding					893:899	differential binding	880:899	differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin)	880:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	12	81	from	specificity	1717:1727	arg1	Culex					1740:1744	Culex	1740:1744	Culex	1740:1744	As BinA displays only mild affinity with receptor polypeptide, we hypothesize that toxin-receptor specificity of BinA in Culex may be mediated by dual interaction of BinA with glycan core of GPI anchor and receptor polypeptide.					
30003921	1	82	theme	Lysinibacillus	111:124	arg1	toxin					154:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin	111:158	Lysinibacillus sphaericus parasporal BinAB toxin displays mosquito larvicidal activity against Culex and Anopheles, but several Aedes species are refractory.					
30003921	7	83	dep	glycoproteins	914:926	arg1	asialofetuin					937:948	asialofetuin	937:948	asialofetuin	937:948	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	83	dep	glycoproteins	914:926	arg1	fetuin					929:934	fetuin	929:934	fetuin	929:934	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	83	dep	glycoproteins	914:926	arg1	glycoproteins					914:926	glycoproteins	914:926	glycoproteins (fetuin, asialofetuin and thyroglobulin)	914:967	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	83	dep	glycoproteins	914:926	arg1	thyroglobulin					954:966	thyroglobulin	954:966	thyroglobulin	954:966	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	4	84	theme	shift	608:612	arg1	assay					614:618	extrinsic fluorescence and thermofluor shift assay	569:618	assay	614:618	We attempted to characterize carbohydrate specificity of BinA by high-throughput approaches using extrinsic fluorescence and thermofluor shift assay.					
30003921	10	85	theme	insect	1441:1446	arg1	larvae					1448:1453	insect larvae	1441:1453	insect larvae	1441:1453	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	13	86	theme	larvicidal	1911:1920	arg1	activity					1922:1929	larvicidal activity	1911:1929	larvicidal activity	1911:1929	The study shall be useful for refining strategies for improving larvicidal activity and for broadening target specificity of BinAB toxin.					
30003921	6	87	theme	fluorescence	821:832	arg1	intensity					834:842	the fluorescence intensity	817:842	the fluorescence intensity	817:842	The promising glycans were identified based on significant change in the fluorescence intensity.					
30003921	7	88	theme	plasmon	853:859	arg1	resonance					861:869	Surface plasmon resonance	845:869	Surface plasmon resonance	845:869	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	10	89	attach	linked	1314:1319	arg2	activity					1298:1305	This activity	1293:1305	This activity	1293:1305	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	10	89	attach	linked	1314:1319	arg1	cytotoxicity					1342:1353	its intracellular cytotoxicity	1324:1353	its intracellular cytotoxicity	1324:1353	This activity may be linked to its intracellular cytotoxicity, as protein N-glycosylation is thought to be critical for development and survival of insect larvae.					
30003921	7	90	theme	simple	986:991	arg1	sugars					993:998	simple sugars	986:998	simple sugars	986:998	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	90	theme	simple	986:991	arg1	l-arabinose					1014:1024	l-arabinose	1014:1024	l-arabinose	1014:1024	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	7	90	theme	simple	986:991	arg1	l-fucose					1001:1008	l-fucose	1001:1008	l-fucose	1001:1008	Surface plasmon resonance revealed differential binding of BinA with glycoproteins (fetuin, asialofetuin and thyroglobulin) and affinity for simple sugars, l-fucose and l-arabinose.					
30003921	11	91	theme	stable	1486:1491	arg1	complexes					1493:1501	stable complexes	1486:1501	stable complexes with BinA	1486:1511	The glycoproteins do not form stable complexes with BinA, however, as observed in the pull-down assay using affinity immobilized BinA and in native-PAGE analysis.					
30003921	6	92	theme	significant	795:805	arg1	change					807:812	significant change	795:812	significant change in the fluorescence intensity	795:842	The promising glycans were identified based on significant change in the fluorescence intensity.					
30003921	2	93	attach	presence	327:334	arg2	domain					362:367	N-terminal lectin-like domain	339:367	N-terminal lectin-like domain	339:367	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
30003921	2	93	attach	presence	327:334	arg1	BinA					372:375	BinA	372:375	BinA	372:375	Recently reported crystal structure of BinAB revealed the presence of N-terminal lectin-like domain in BinA.					
31000219	6	0	theme	physicochemical	1362:1376	arg1	exposure					1315:1322	the exposure	1311:1322	the exposure of hydrophobic regions	1311:1345	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	6	0	theme	physicochemical	1362:1376	arg1	characteristic					1378:1391	the leading physicochemical characteristic	1350:1391	the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages	1350:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	2	1	from	60 °C	409:413	arg1	solution					418:425	solution	418:425	solution	418:425	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	6	2	theme	increased	1257:1265	arg1	hydrophobicity					1267:1280	the increased hydrophobicity	1253:1280	the increased hydrophobicity	1253:1280	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	2	3	theme	different	344:352	arg1	methods					365:371	3 different processing methods	342:371	3 different processing methods	342:371	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	1	4	theme	protein	237:243	arg1	structure					245:253	food protein structure	232:253	food protein structure	232:253	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	3	5	theme	THP-1	655:659	arg1	macrophages					661:671	THP-1 macrophages	655:671	THP-1 macrophages	655:671	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	4	6	theme	samples	814:820	arg1	parameters					790:799	physicochemical parameters	774:799	physicochemical parameters of processed samples	774:820	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	6	7	theme	regions	1339:1345	arg1	characteristic					1378:1391	the leading physicochemical characteristic	1350:1391	the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages	1350:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	6	7	theme	regions	1339:1345	arg1	exposure					1315:1322	the exposure	1311:1322	the exposure of hydrophobic regions	1311:1345	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	6	8	theme	THP-1	1446:1450	arg1	macrophages					1452:1462	THP-1 macrophages	1446:1462	THP-1 macrophages	1446:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	1	9	theme	structure	245:253	arg1	modifications					215:227	modifications	215:227	modifications of food protein structure	215:253	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	2	10	theme	dry	489:491	arg1	heating					493:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	3	11	theme	BLG	648:650	arg1	uptake					638:643	the uptake	634:643	the uptake of BLG by THP-1 macrophages	634:671	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	2	12	theme	saccharides	537:547	arg1	types					528:532	8 types	526:532	8 types of saccharides	526:547	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	6	13	theme	molecular	1220:1228	arg1	weight					1230:1235	molecular weight	1220:1235	molecular weight	1220:1235	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	7	14	contain	have	1568:1571	arg1	processing					1513:1522	differential thermal processing	1492:1522	differential thermal processing of foods	1492:1531	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	7	14	contain	have	1568:1571	arg2	impact					1576:1581	an impact	1573:1581	an impact	1573:1581	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	2	15	dep	heating	493:499	arg1	50 °C					468:472	50 °C	468:472	50 °C	468:472	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	2	15	dep	heating	493:499	arg1	130 °C					458:463	130 °C	458:463	130 °C	458:463	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	5	16	theme	wet	1045:1047	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	3	17	contain	had	708:710	arg1	type					684:687	the type	680:687	the type of saccharide	680:701	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	3	17	contain	had	708:710	arg2	influence					715:723	an influence	712:723	an influence	712:723	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	0	18	theme	processed	96:104	arg1	β-lactoglobulin					106:120	thermally processed β-lactoglobulin	86:120	thermally processed β-lactoglobulin	86:120	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	5	19	theme	heating	1049:1055	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	4	20	theme	parameters	790:799	arg1	set					767:769	A set	765:769	A set of physicochemical parameters of processed samples	765:820	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	20	theme	parameters	790:799	arg1	parameters					790:799	physicochemical parameters	774:799	physicochemical parameters of processed samples	774:820	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	5	21	attach	linked	1031:1036	arg1	percentage					1079:1088	percentage	1079:1088	percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1079:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	21	attach	linked	1031:1036	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	21	attach	linked	1031:1036	arg2	uptake					1018:1023	the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1014:1164	the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1014:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	4	22	theme	amyloid-like	898:909	arg1	structure					911:919	amyloid-like structure	898:919	amyloid-like structure	898:919	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	22	theme	amyloid-like	898:909	arg1	weight					874:879	the samples' molecular weight	851:879	the samples' molecular weight	851:879	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	5	23	theme	processing	1057:1066	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	4	24	theme	physicochemical	774:788	arg1	parameters					790:799	physicochemical parameters	774:799	physicochemical parameters of processed samples	774:820	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	1	25	theme	study	160:164	arg1	aim					148:150	The aim	144:150	The aim of this study	144:164	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	6	26	theme	samples	1435:1441	arg1	uptake					1410:1415	the observed uptake	1397:1415	the observed uptake of wet heated BLG samples by THP-1 macrophages	1397:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	6	27	theme	leading	1354:1360	arg1	exposure					1315:1322	the exposure	1311:1322	the exposure of hydrophobic regions	1311:1345	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	6	27	theme	leading	1354:1360	arg1	characteristic					1378:1391	the leading physicochemical characteristic	1350:1391	the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages	1350:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	4	28	theme	processed	804:812	arg1	samples					814:820	processed samples	804:820	processed samples	804:820	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	3	29	theme	saccharide	692:701	arg1	type					684:687	the type	680:687	the type of saccharide	680:701	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	6	30	theme	BLG	1431:1433	arg1	samples					1435:1441	wet heated BLG samples	1420:1441	wet heated BLG samples	1420:1441	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	1	31	theme	thermal	262:268	arg1	processing					270:279	thermal processing	262:279	thermal processing	262:279	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	7	32	theme	protein	1542:1548	arg1	modification					1550:1561	protein modification	1542:1561	protein modification	1542:1561	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	6	33	theme	heated	1424:1429	arg1	samples					1435:1441	wet heated BLG samples	1420:1441	wet heated BLG samples	1420:1441	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	5	34	theme	polymers	1137:1144	arg1	percentage					1079:1088	percentage	1079:1088	percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1079:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	34	theme	polymers	1137:1144	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	4	35	theme	molecular	864:872	arg1	charge					930:935	surface charge	922:935	surface charge	922:935	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	35	theme	molecular	864:872	arg1	hydrophobicity					882:895	hydrophobicity	882:895	hydrophobicity	882:895	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	35	theme	molecular	864:872	arg1	structure					911:919	amyloid-like structure	898:919	amyloid-like structure	898:919	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	35	theme	molecular	864:872	arg1	weight					874:879	the samples' molecular weight	851:879	the samples' molecular weight	851:879	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	35	theme	molecular	864:872	arg1	structure					951:959	secondary structure	941:959	secondary structure	941:959	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	5	36	theme	alterations	992:1002	arg1	Analysis					962:969	Analysis	962:969	Analysis of protein structure alterations	962:1002	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	7	37	with	interaction	1590:1600	arg1	system					1618:1623	the immune system	1607:1623	the immune system	1607:1623	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	6	38	theme	wet	1420:1422	arg1	samples					1435:1441	wet heated BLG samples	1420:1441	wet heated BLG samples	1420:1441	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	0	39	theme	β-lactoglobulin	106:120	arg1	uptake					76:81	uptake	76:81	uptake of thermally processed β-lactoglobulin by THP-1 macrophages	76:141	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	4	40	theme	surface	922:928	arg1	weight					874:879	the samples' molecular weight	851:879	the samples' molecular weight	851:879	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	40	theme	surface	922:928	arg1	charge					930:935	surface charge	922:935	surface charge	922:935	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	2	41	theme	low-temperature	441:455	arg1	heating					493:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	1	42	theme	immunological	188:200	arg1	relevance					202:210	the immunological relevance	184:210	the immunological relevance of modifications of food protein structure due to thermal processing	184:279	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	2	43	theme	wet	396:398	arg1	heating					400:406	wet heating	396:406	wet heating (60 °C in solution)	396:426	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	4	44	theme	secondary	941:949	arg1	weight					874:879	the samples' molecular weight	851:879	the samples' molecular weight	851:879	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	4	44	theme	secondary	941:949	arg1	structure					951:959	secondary structure	941:959	secondary structure	941:959	A set of physicochemical parameters of processed samples was determined, to determine the samples' molecular weight, hydrophobicity, amyloid-like structure, surface charge and secondary structure.					
31000219	5	45	theme	structure	1101:1109	arg1	percentage					1079:1088	percentage	1079:1088	percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1079:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	45	theme	structure	1101:1109	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	0	46	theme	THP-1	125:129	arg1	macrophages					131:141	THP-1 macrophages	125:141	THP-1 macrophages	125:141	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	7	47	theme	foods	1527:1531	arg1	processing					1513:1522	differential thermal processing	1492:1522	differential thermal processing of foods	1492:1531	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	5	48	theme	protein	974:980	arg1	alterations					992:1002	protein structure alterations	974:1002	protein structure alterations	974:1002	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	6	49	theme	hydrophobic	1327:1337	arg1	regions					1339:1345	hydrophobic regions	1327:1345	hydrophobic regions	1327:1345	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	2	50	theme	high-	432:436	arg1	heating					493:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	high- or low-temperature (130 °C or 50 °C, respectively) dry heating	432:499	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	7	51	theme	immune	1611:1616	arg1	system					1618:1623	the immune system	1607:1623	the immune system	1607:1623	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	5	52	theme	structure	982:990	arg1	alterations					992:1002	protein structure alterations	974:1002	protein structure alterations	974:1002	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	53	theme	α-helix	1093:1099	arg1	structure					1101:1109	α-helix structure	1093:1109	α-helix structure	1093:1109	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	54	theme	amyloid-like	1112:1123	arg1	structures					1125:1134	amyloid-like structures	1112:1134	amyloid-like structures	1112:1134	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	2	55	dep	heating	400:406	arg1	60 °C					409:413	60 °C	409:413	60 °C in solution	409:425	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	5	56	theme	structures	1125:1134	arg1	percentage					1079:1088	percentage	1079:1088	percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1079:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	56	theme	structures	1125:1134	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	6	57	theme	amyloid-like	1192:1203	arg1	structures					1205:1214	amyloid-like structures	1192:1214	amyloid-like structures	1192:1214	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	3	58	theme	heated	749:754	arg1	samples					756:762	high-temperature dry heated samples	728:762	high-temperature dry heated samples	728:762	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	7	59	theme	differential	1492:1503	arg1	processing					1513:1522	differential thermal processing	1492:1522	differential thermal processing of foods	1492:1531	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	3	60	theme	processing	576:585	arg1	method					587:592	The processing method	572:592	The processing method that was applied	572:609	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	5	61	theme	hydrophobicity	1151:1164	arg1	percentage					1079:1088	percentage	1079:1088	percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity	1079:1164	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	5	61	theme	hydrophobicity	1151:1164	arg1	method					1068:1073	the wet heating processing method	1041:1073	the wet heating processing method	1041:1073	Analysis of protein structure alterations indicated the uptake to be linked to the wet heating processing method and percentage of α-helix structure, amyloid-like structures, polymers, and hydrophobicity.					
31000219	2	62	theme	β-lactoglobulin	312:326	arg1	uptake					302:307	the uptake	298:307	the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages	298:393	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	1	63	theme	modifications	215:227	arg1	relevance					202:210	the immunological relevance	184:210	the immunological relevance of modifications of food protein structure due to thermal processing	184:279	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
31000219	0	64	theme	key	55:57	arg1	determinants					59:70	key determinants	55:70	key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages	55:141	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	0	64	theme	key	55:57	arg1	aggregation					19:29	aggregation	19:29	aggregation	19:29	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	0	64	theme	key	55:57	arg1	Hydrophobicity					0:13	Hydrophobicity	0:13	Hydrophobicity	0:13	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	0	64	theme	key	55:57	arg1	glycation					40:48	glycation	40:48	glycation	40:48	Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages.					
31000219	7	65	theme	thermal	1505:1511	arg1	processing					1513:1522	differential thermal processing	1492:1522	differential thermal processing of foods	1492:1531	This work demonstrates how differential thermal processing of foods, through protein modification, can have an impact on its interaction with the immune system.					
31000219	2	66	theme	THP-1	377:381	arg1	macrophages					383:393	THP-1 macrophages	377:393	THP-1 macrophages	377:393	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	3	67	theme	high-temperature	728:743	arg1	samples					756:762	high-temperature dry heated samples	728:762	high-temperature dry heated samples	728:762	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	6	68	theme	observed	1401:1408	arg1	uptake					1410:1415	the observed uptake	1397:1415	the observed uptake of wet heated BLG samples by THP-1 macrophages	1397:1462	We hypothesize that both amyloid-like structures and molecular weight were related to the increased hydrophobicity and therefore postulate that the exposure of hydrophobic regions is the leading physicochemical characteristic for the observed uptake of wet heated BLG samples by THP-1 macrophages.					
31000219	3	69	theme	dry	745:747	arg1	samples					756:762	high-temperature dry heated samples	728:762	high-temperature dry heated samples	728:762	The processing method that was applied significantly affected the uptake of BLG by THP-1 macrophages, while the type of saccharide only had an influence in high-temperature dry heated samples.					
31000219	2	70	theme	processing	354:363	arg1	methods					365:371	3 different processing methods	342:371	3 different processing methods	342:371	We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 macrophages: wet heating (60 °C in solution) and high- or low-temperature (130 °C or 50 °C, respectively) dry heating, combined with either of 8 types of saccharides or without saccharide.					
31000219	1	71	theme	food	232:235	arg1	structure					245:253	food protein structure	232:253	food protein structure	232:253	The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing.					
30663306	1	0	theme	subsequent	318:327	arg1	colonization					329:340	subsequent colonization	318:340	subsequent colonization of microbes	318:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	3	1	theme	bovine	688:693	arg1	BCF					715:717	BCF	715:717	BCF	715:717	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	3	1	theme	bovine	688:693	arg1	fraction					705:712	a bovine colostrum fraction	686:712	a bovine colostrum fraction (BCF)	686:718	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	3	2	theme	colostrum	695:703	arg1	BCF					715:717	BCF	715:717	BCF	715:717	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	3	2	theme	colostrum	695:703	arg1	fraction					705:712	a bovine colostrum fraction	686:712	a bovine colostrum fraction (BCF)	686:718	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	0	3	theme	Cells	78:82	arg1	Proteome					44:51	the Proteome	40:51	the Proteome of Intestinal Epithelial Cells	40:82	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	3	4	theme	cells	662:666	arg1	receptor					608:615	the surface receptor and glycan profile	596:634	receptor	608:615	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	3	4	theme	cells	662:666	arg1	profile					628:634	the surface receptor and glycan profile	596:634	profile	628:634	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	4	5	theme	profiles	840:847	arg1	Integration					765:775	Integration	765:775	Integration of label-free quantitative proteomic analysis and lectin array profiles	765:847	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	2	6	theme	post-translational	495:512	arg1	modifications					514:526	the post-translational modifications	491:526	the post-translational modifications associated with them	491:547	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	1	7	from	preparing	288:296	arg1	important					192:200	important	192:200	important	192:200	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	8	theme	gut	254:256	arg1	structure					258:266	gut structure	254:266	gut structure	254:266	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	0	9	theme	Integrated	87:96	arg1	Proteomics					98:107	Proteomics	98:107	Proteomics	98:107	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	1	10	theme	microbes	345:352	arg1	colonization					329:340	subsequent colonization	318:340	subsequent colonization of microbes	318:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	11	theme	bioactive	161:169	arg1	components					171:180	bioactive components	161:180	bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes	161:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	2	12	theme	surface	479:485	arg1	receptors					429:437	receptors	429:437	receptors	429:437	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	12	theme	surface	479:485	arg1	proteins					452:459	membrane proteins	443:459	membrane proteins	443:459	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	5	13	theme	intestinal	1084:1093	arg1	cells					1095:1099	intestinal cells	1084:1099	intestinal cells	1084:1099	This study contributes to our understanding of how milk components may regulate intestinal cells and prime them for bacterial interaction.					
30663306	4	14	theme	analysis	814:821	arg1	profiles					840:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	1	15	theme	components	171:180	arg1	colostrum					131:139	Bovine colostrum	124:139	Bovine colostrum	124:139	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	15	theme	components	171:180	arg1	source					151:156	a rich source	144:156	a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes	144:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	2	16	theme	proteins	452:459	arg1	receptors					429:437	receptors	429:437	receptors	429:437	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	16	theme	proteins	452:459	arg1	proteins					452:459	membrane proteins	443:459	membrane proteins	443:459	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	16	theme	proteins	452:459	arg1	repertoire					415:424	the repertoire	411:424	the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them	411:547	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	4	17	theme	array	834:838	arg1	profiles					840:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	2	18	theme	intestinal	468:477	arg1	surface					479:485	the intestinal surface	464:485	the intestinal surface	464:485	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	4	19	gly	glycoproteins	913:925	arg1	glycoproteins					913:925	glycoproteins	913:925	glycoproteins present at the cell surface	913:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	20	theme	lectin	827:832	arg1	array					834:838	lectin array	827:838	lectin array	827:838	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	21	theme	glycosylation	981:993	arg1	pattern					995:1001	their glycosylation pattern	975:1001	their glycosylation pattern	975:1001	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	3	22	theme	present	557:563	arg1	work					565:568	the present work	553:568	the present work	553:568	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	0	23	theme	Changes	29:35	arg1	Interrogation					0:12	Interrogation	0:12	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.	0:122	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	4	24	from	changes	888:894	arg1	levels					903:908	the levels	899:908	the levels of glycoproteins present at the cell surface	899:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	25	theme	label-free	780:789	arg1	profiles					840:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	label-free quantitative proteomic analysis and lectin array profiles	780:847	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	0	26	theme	Milk-Driven	17:27	arg1	Changes					29:35	Milk-Driven Changes	17:35	Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells	17:82	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	3	27	theme	glycan	621:626	arg1	profile					628:634	the surface receptor and glycan profile	596:634	profile	628:634	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	2	28	theme	membrane	443:450	arg1	proteins					452:459	membrane proteins	443:459	membrane proteins	443:459	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	29	dep	is	383:384	arg1	clear					367:371	clear	367:371	clear	367:371	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	3	30	theme	proteomic	732:740	arg1	analyses					755:762	proteomic and glycomic analyses	732:762	proteomic and glycomic analyses	732:762	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	1	31	from	stimulating	242:252	arg1	important					192:200	important	192:200	important	192:200	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	5	32	theme	milk	1055:1058	arg1	components					1060:1069	milk components	1055:1069	milk components	1055:1069	This study contributes to our understanding of how milk components may regulate intestinal cells and prime them for bacterial interaction.					
30663306	3	33	theme	glycomic	746:753	arg1	analyses					755:762	proteomic and glycomic analyses	732:762	proteomic and glycomic analyses	732:762	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	2	34	theme	receptors	429:437	arg1	receptors					429:437	receptors	429:437	receptors	429:437	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	34	theme	receptors	429:437	arg1	proteins					452:459	membrane proteins	443:459	membrane proteins	443:459	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	34	theme	receptors	429:437	arg1	repertoire					415:424	the repertoire	411:424	the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them	411:547	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	4	35	theme	present	927:933	arg1	glycoproteins					913:925	glycoproteins	913:925	glycoproteins present at the cell surface	913:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	36	theme	BCF	864:866	arg1	exposure					868:875	BCF exposure	864:875	BCF exposure	864:875	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	37	theme	cell	942:945	arg1	surface					947:953	the cell surface	938:953	the cell surface	938:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	38	theme	proteomic	804:812	arg1	analysis					814:821	quantitative proteomic analysis	791:821	quantitative proteomic analysis	791:821	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	1	39	from	development	209:219	arg1	important					192:200	important	192:200	important	192:200	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	2	40	theme	modifications	514:526	arg1	receptors					429:437	receptors	429:437	receptors	429:437	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	2	40	theme	modifications	514:526	arg1	proteins					452:459	membrane proteins	443:459	membrane proteins	443:459	What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them.					
30663306	1	41	theme	gut	302:304	arg1	surface					306:312	the gut surface	298:312	the gut surface	298:312	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	3	42	theme	HT-29	645:649	arg1	cells					662:666	human HT-29 intestinal cells	639:666	human HT-29 intestinal cells	639:666	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	4	43	theme	quantitative	791:802	arg1	analysis					814:821	quantitative proteomic analysis	791:821	quantitative proteomic analysis	791:821	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	1	44	from	important	192:200	arg1	stimulating					242:252	stimulating	242:252	stimulating gut structure and function	242:279	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	44	from	important	192:200	arg1	preparing					288:296	preparing	288:296	preparing the gut surface for subsequent colonization of microbes	288:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	44	from	important	192:200	arg1	development					209:219	the development	205:219	the development of the intestine	205:236	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	45	theme	Bovine	124:129	arg1	colostrum					131:139	Bovine colostrum	124:139	Bovine colostrum	124:139	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	45	theme	Bovine	124:129	arg1	source					151:156	a rich source	144:156	a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes	144:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	4	46	from	surface	947:953	arg1	present					927:933	present	927:933	present	927:933	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	5	47	theme	bacterial	1120:1128	arg1	interaction					1130:1140	bacterial interaction	1120:1140	bacterial interaction	1120:1140	This study contributes to our understanding of how milk components may regulate intestinal cells and prime them for bacterial interaction.					
30663306	3	48	theme	intestinal	651:660	arg1	cells					662:666	human HT-29 intestinal cells	639:666	human HT-29 intestinal cells	639:666	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	3	49	theme	surface	600:606	arg1	receptor					608:615	the surface receptor and glycan profile	596:634	receptor	608:615	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	0	50	theme	Epithelial	67:76	arg1	Cells					78:82	Intestinal Epithelial Cells	56:82	Intestinal Epithelial Cells	56:82	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	3	51	theme	human	639:643	arg1	cells					662:666	human HT-29 intestinal cells	639:666	human HT-29 intestinal cells	639:666	In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses.					
30663306	4	52	attach	present	927:933	arg2	glycoproteins					913:925	glycoproteins	913:925	glycoproteins present at the cell surface	913:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	4	52	attach	present	927:933	arg1	surface					947:953	the cell surface	938:953	the cell surface	938:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
30663306	1	53	theme	intestine	228:236	arg1	development					209:219	the development	205:219	the development of the intestine	205:236	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	0	54	theme	Intestinal	56:65	arg1	Cells					78:82	Intestinal Epithelial Cells	56:82	Intestinal Epithelial Cells	56:82	Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics.					
30663306	1	55	theme	rich	146:149	arg1	colostrum					131:139	Bovine colostrum	124:139	Bovine colostrum	124:139	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	1	55	theme	rich	146:149	arg1	source					151:156	a rich source	144:156	a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes	144:352	Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes.					
30663306	4	56	theme	glycoproteins	913:925	arg1	levels					903:908	the levels	899:908	the levels of glycoproteins present at the cell surface	899:953	Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern.					
29410168	0	0	theme	composite	74:82	arg1	mitigation					51:60	corrosion mitigation	41:60	corrosion mitigation of aluminum composite	41:82	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	4	1	theme	morphology	685:694	arg1	studies					696:702	Surface morphology studies	677:702	Surface morphology studies	677:702	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	1	2	theme	corrosion	147:155	arg1	ability					168:174	the corrosion inhibition ability	143:174	the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	143:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	0	3	theme	aluminum	65:72	arg1	composite					74:82	aluminum composite	65:82	aluminum composite	65:82	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	3	4	theme	323K	671:674	arg1	range					656:660	the temperature range	640:660	the temperature range of 308K- 323K	640:674	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	8	5	theme	corrosion	1264:1272	arg1	control					1274:1280	the corrosion control	1260:1280	the corrosion control of Al-CM	1260:1289	Pullulan can be considered as effective, eco friendly green inhibitor for the corrosion control of Al-CM.					
29410168	1	6	theme	inhibition	157:166	arg1	ability					168:174	the corrosion inhibition ability	143:174	the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	143:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	6	7	theme	parameters	958:967	arg1	Evaluation					918:927	Evaluation	918:927	Evaluation of kinetic and thermodynamic parameters	918:967	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	6	8	theme	adsorption	1071:1080	arg1	isotherm					1082:1089	Freundlich adsorption isotherm	1060:1089	Freundlich adsorption isotherm	1060:1089	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	5	9	from	303K	870:873	arg1	efficiency					849:858	a maximum efficiency	839:858	a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor	839:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	5	9	from	303K	870:873	arg1	%					865:865	89%	863:865	89% at 303K for the addition of 1.0 gL-1 of inhibitor	863:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	5	10	theme	inhibitor	907:915	arg1	gL-1					899:902	1.0 gL-1	895:902	1.0 gL-1 of inhibitor	895:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	6	11	theme	thermodynamic	944:956	arg1	parameters					958:967	kinetic and thermodynamic parameters	932:967	kinetic and thermodynamic parameters	932:967	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	1	12	dep	composite	285:293	arg1	%					273:273	6061Aluminum-15%	258:273	6061Aluminum-15%	258:273	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	4	13	theme	inhibitor	744:752	arg1	adsorption					730:739	the adsorption	726:739	the adsorption of inhibitor on the surface of composite material	726:789	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	2	14	theme	electrochemical	317:331	arg1	studies					438:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	14	theme	electrochemical	317:331	arg1	measurements					333:344	The electrochemical measurements	313:344	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	313:444	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	0	15	theme	surface	105:111	arg1	studies					113:119	surface studies	105:119	surface studies	105:119	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	0	16	dep	Electrochemical	85:99	arg1	Pullulan					0:7	Pullulan	0:7	Pullulan	0:7	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	2	17	theme	potentiodynamic	354:368	arg1	polarization					370:381	potentiodynamic polarization	354:381	potentiodynamic polarization (PDP)	354:387	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	17	theme	potentiodynamic	354:368	arg1	PDP					384:386	PDP	384:386	PDP	384:386	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	18	theme	inhibition	481:490	arg1	studies					492:498	the corrosion inhibition studies	467:498	the corrosion inhibition studies	467:498	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	6	19	theme	Freundlich	1060:1069	arg1	isotherm					1082:1089	Freundlich adsorption isotherm	1060:1089	Freundlich adsorption isotherm	1060:1089	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	2	20	theme	polarization	370:381	arg1	studies					438:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	4	21	from	adsorption	730:739	arg1	surface					761:767	the surface	757:767	the surface of composite material	757:789	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	6	22	theme	Al-CM	1043:1047	arg1	surface					1032:1038	the surface	1028:1038	the surface of Al-CM	1028:1047	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	1	23	theme	pullulan	179:186	arg1	ability					168:174	the corrosion inhibition ability	143:174	the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	143:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	7	24	theme	pullulan	1167:1174	arg1	molecule					1176:1183	pullulan molecule	1167:1183	pullulan molecule	1167:1183	The surface characterization like SEM-EDX, AFM confirmed the adsorption of pullulan molecule.					
29410168	6	25	theme	kinetic	932:938	arg1	parameters					958:967	kinetic and thermodynamic parameters	932:967	kinetic and thermodynamic parameters	932:967	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	5	26	theme	gL-1	899:902	arg1	addition					883:890	the addition	879:890	the addition of 1.0 gL-1 of inhibitor	879:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	4	27	theme	Surface	677:683	arg1	studies					696:702	Surface morphology studies	677:702	Surface morphology studies	677:702	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	0	28	theme	potent	14:19	arg1	inhibitor					27:35	a potent green inhibitor	12:35	a potent green inhibitor for corrosion mitigation of aluminum composite	12:82	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	8	29	dep	effective	1216:1224	arg1	green					1240:1244	green	1240:1244	green	1240:1244	Pullulan can be considered as effective, eco friendly green inhibitor for the corrosion control of Al-CM.					
29410168	3	30	theme	maximum	537:543	arg1	efficiency					556:565	maximum inhibition efficiency	537:565	maximum inhibition efficiency	537:565	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	2	31	theme	electrochemical	393:407	arg1	EIS					433:435	EIS	433:435	EIS	433:435	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	31	theme	electrochemical	393:407	arg1	spectroscopy					419:430	electrochemical impedance spectroscopy	393:430	electrochemical impedance spectroscopy (EIS)	393:436	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	32	theme	impedance	409:417	arg1	EIS					433:435	EIS	433:435	EIS	433:435	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	2	32	theme	impedance	409:417	arg1	spectroscopy					419:430	electrochemical impedance spectroscopy	393:430	electrochemical impedance spectroscopy (EIS)	393:436	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	3	33	theme	inhibition	545:554	arg1	efficiency					556:565	maximum inhibition efficiency	537:565	maximum inhibition efficiency	537:565	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	5	34	theme	inhibitor	824:832	arg1	type					816:819	mixed type	810:819	mixed type of inhibitor	810:832	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	4	35	theme	material	782:789	arg1	surface					761:767	the surface	757:767	the surface of composite material	757:789	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	1	36	theme	benign	208:213	arg1	pullulan					179:186	pullulan	179:186	pullulan	179:186	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	1	36	theme	benign	208:213	arg1	polysaccharide					222:235	an environmentally benign fungal polysaccharide	189:235	an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	189:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	0	37	theme	green	21:25	arg1	inhibitor					27:35	a potent green inhibitor	12:35	a potent green inhibitor for corrosion mitigation of aluminum composite	12:82	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	3	38	theme	inhibitor	626:634	arg1	concentrations					608:621	varying concentrations	600:621	varying concentrations of inhibitor	600:634	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	2	39	theme	spectroscopy	419:430	arg1	studies					438:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies	354:444	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	1	40	theme	fungal	215:220	arg1	pullulan					179:186	pullulan	179:186	pullulan	179:186	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	1	40	theme	fungal	215:220	arg1	polysaccharide					222:235	an environmentally benign fungal polysaccharide	189:235	an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	189:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	0	41	theme	corrosion	41:49	arg1	mitigation					51:60	corrosion mitigation	41:60	corrosion mitigation of aluminum composite	41:82	Pullulan as a potent green inhibitor for corrosion mitigation of aluminum composite: Electrochemical and surface studies.					
29410168	1	42	theme	composite	285:293	arg1	Al-CM					305:309	Al-CM	305:309	Al-CM	305:309	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	1	42	theme	composite	285:293	arg1	material					295:302	6061Aluminum-15%(v) SiC(P) composite material	258:302	6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	258:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	1	43	theme	material	295:302	arg1	corrosion					245:253	acid corrosion	240:253	acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	240:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	1	44	from	polysaccharide	222:235	arg1	corrosion					245:253	acid corrosion	240:253	acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	240:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	3	45	theme	308K-	665:669	arg1	323K					671:674	308K- 323K	665:674	308K- 323K	665:674	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	4	46	theme	composite	772:780	arg1	material					782:789	composite material	772:789	composite material	772:789	Surface morphology studies were done to reaffirm the adsorption of inhibitor on the surface of composite material.					
29410168	6	47	theme	physical	1003:1010	arg1	adsorption					1012:1021	physical adsorption	1003:1021	physical adsorption onto the surface of Al-CM	1003:1047	Evaluation of kinetic and thermodynamic parameters revealed that inhibitor underwent physical adsorption onto the surface of Al-CM and obeyed Freundlich adsorption isotherm.					
29410168	3	48	theme	temperature	644:654	arg1	range					656:660	the temperature range	640:660	the temperature range of 308K- 323K	640:674	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	5	49	theme	mixed	810:814	arg1	type					816:819	mixed type	810:819	mixed type of inhibitor	810:832	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	5	50	from	efficiency	849:858	arg1	303K					870:873	303K	870:873	303K for the addition of 1.0 gL-1 of inhibitor	870:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	8	51	theme	Al-CM	1285:1289	arg1	control					1274:1280	the corrosion control	1260:1280	the corrosion control of Al-CM	1260:1289	Pullulan can be considered as effective, eco friendly green inhibitor for the corrosion control of Al-CM.					
29410168	2	52	theme	corrosion	471:479	arg1	studies					492:498	the corrosion inhibition studies	467:498	the corrosion inhibition studies	467:498	The electrochemical measurements such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) studies were carried out for the corrosion inhibition studies.					
29410168	5	53	theme	maximum	841:847	arg1	efficiency					849:858	a maximum efficiency	839:858	a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor	839:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	1	54	theme	acid	240:243	arg1	corrosion					245:253	acid corrosion	240:253	acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM)	240:310	This work emphasizes the corrosion inhibition ability of pullulan, an environmentally benign fungal polysaccharide on acid corrosion of 6061Aluminum-15%(v) SiC(P) composite material (Al-CM).					
29410168	8	55	theme	effective	1216:1224	arg1	Pullulan					1186:1193	Pullulan	1186:1193	Pullulan	1186:1193	Pullulan can be considered as effective, eco friendly green inhibitor for the corrosion control of Al-CM.					
29410168	8	55	theme	effective	1216:1224	arg1	inhibitor					1246:1254	effective, eco friendly green inhibitor	1216:1254	effective, eco friendly green inhibitor for the corrosion control of Al-CM	1216:1289	Pullulan can be considered as effective, eco friendly green inhibitor for the corrosion control of Al-CM.					
29410168	7	56	theme	molecule	1176:1183	arg1	adsorption					1153:1162	the adsorption	1149:1162	the adsorption of pullulan molecule	1149:1183	The surface characterization like SEM-EDX, AFM confirmed the adsorption of pullulan molecule.					
29410168	3	57	theme	varying	600:606	arg1	concentrations					608:621	varying concentrations	600:621	varying concentrations of inhibitor	600:634	Conditions were optimized to obtain maximum inhibition efficiency, by performing the experiment at varying concentrations of inhibitor, in the temperature range of 308K- 323K.					
29410168	5	58	theme	%	865:865	arg1	efficiency					849:858	a maximum efficiency	839:858	a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor	839:915	Pullulan acted as mixed type of inhibitor with a maximum efficiency of 89% at 303K for the addition of 1.0 gL-1 of inhibitor.					
29410168	7	59	theme	surface	1096:1102	arg1	characterization					1104:1119	The surface characterization	1092:1119	The surface characterization like SEM-EDX, AFM	1092:1137	The surface characterization like SEM-EDX, AFM confirmed the adsorption of pullulan molecule.					
31040271	0	0	theme	CHO	89:91	arg1	cells					93:97	CHO cells	89:97	CHO cells	89:97	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	4	1	from	screen	643:648	arg1	cells					675:679	Chinese hamster ovary cells	653:679	Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	653:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	1	2	theme	rare	168:171	arg1	deficiencies					201:212	rare congenital lysosomal enzyme deficiencies	168:212	rare congenital lysosomal enzyme deficiencies	168:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	5	3	theme	mouse	955:959	arg1	model					961:965	a Fabry disease mouse model	939:965	a Fabry disease mouse model	939:965	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	1	4	theme	congenital	173:182	arg1	deficiencies					201:212	rare congenital lysosomal enzyme deficiencies	168:212	rare congenital lysosomal enzyme deficiencies	168:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	5	5	gly	sialylated	990:999	arg1	glycoform					1001:1009	an α2-3 sialylated glycoform	982:1009	an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors	982:1087	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	6	6	theme	enzyme	1280:1285	arg1	therapeutics					1299:1310	enzyme replacement therapeutics	1280:1310	enzyme replacement therapeutics	1280:1310	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	4	7	theme	enzymes	718:724	arg1	production					694:703	production	694:703	production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	694:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	3	8	theme	different	567:575	arg1	features					584:591	different glycan features	567:591	different glycan features	567:591	It has not been possible to design and systematically study effects of different glycan features.					
31040271	4	9	theme	gene	626:629	arg1	screen					643:648	a comprehensive gene engineering screen	610:648	a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	610:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	6	10	theme	cell	1224:1227	arg1	lines					1229:1233	engineered CHO cell lines	1209:1233	engineered CHO cell lines	1209:1233	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	6	11	theme	CHO	1220:1222	arg1	lines					1229:1233	engineered CHO cell lines	1209:1233	engineered CHO cell lines	1209:1233	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	3	12	theme	glycan	577:582	arg1	features					584:591	different glycan features	567:591	different glycan features	567:591	It has not been possible to design and systematically study effects of different glycan features.					
31040271	2	13	theme	cell	386:389	arg1	receptors					406:414	cell surface glycan receptors	386:414	cell surface glycan receptors	386:414	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
31040271	1	14	theme	short	279:283	arg1	half-life					297:305	circulatory half-life	285:305	circulatory half-life	285:305	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	5	15	theme	selected	895:902	arg1	glycoforms					904:913	selected glycoforms	895:913	selected glycoforms of α-galactosidase A	895:934	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	1	16	theme	lysosomal	184:192	arg1	deficiencies					201:212	rare congenital lysosomal enzyme deficiencies	168:212	rare congenital lysosomal enzyme deficiencies	168:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	4	17	theme	comprehensive	612:624	arg1	screen					643:648	a comprehensive gene engineering screen	610:648	a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	610:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	6	18	theme	developed	1181:1189	arg1	matrix					1198:1203	The developed design matrix	1177:1203	The developed design matrix	1177:1203	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	2	19	theme	circulation	478:488	arg1	time					490:493	circulation time	478:493	circulation time	478:493	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
31040271	1	20	theme	enzyme	194:199	arg1	deficiencies					201:212	rare congenital lysosomal enzyme deficiencies	168:212	rare congenital lysosomal enzyme deficiencies	168:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	0	21	theme	glycosylation	4:16	arg1	space					25:29	The glycosylation design space	0:29	The glycosylation design space for recombinant lysosomal replacement enzymes	0:75	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	4	22	with	enzymes	718:724	arg1	N-glycans					731:739	N-glycans	731:739	N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	731:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	4	23	theme	cellular	795:802	arg1	uptake					804:809	cellular uptake	795:809	cellular uptake	795:809	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	4	24	theme	lysosomal	708:716	arg1	enzymes					718:724	lysosomal enzymes	708:724	lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	708:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	1	25	theme	circulatory	285:295	arg1	half-life					297:305	circulatory half-life	285:305	circulatory half-life	285:305	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	5	26	gly	glycoforms	904:913	arg1	A					934:934	α-galactosidase A	918:934	α-galactosidase A	918:934	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	27	theme	α-galactosidase	918:932	arg1	A					934:934	α-galactosidase A	918:934	α-galactosidase A	918:934	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	2	28	theme	Replacement	340:350	arg1	enzymes					352:358	Replacement enzymes	340:358	Replacement enzymes	340:358	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
31040271	5	29	theme	organ	873:877	arg1	distribution					879:890	organ distribution	873:890	organ distribution	873:890	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	4	30	theme	glycan	771:776	arg1	features					778:785	key glycan features	767:785	key glycan features guiding cellular uptake and circulation	767:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	1	31	theme	Lysosomal	100:108	arg1	enzymes					122:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	1	31	theme	Lysosomal	100:108	arg1	options					156:162	essential therapeutic options	134:162	essential therapeutic options for rare congenital lysosomal enzyme deficiencies	134:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	4	32	theme	ovary	669:673	arg1	cells					675:679	Chinese hamster ovary cells	653:679	Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	653:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	0	33	theme	design	18:23	arg1	space					25:29	The glycosylation design space	0:29	The glycosylation design space for recombinant lysosomal replacement enzymes	0:75	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	5	34	theme	sialylated	990:999	arg1	glycoform					1001:1009	an α2-3 sialylated glycoform	982:1009	an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors	982:1087	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	1	35	theme	replacement	110:120	arg1	enzymes					122:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	1	35	theme	replacement	110:120	arg1	options					156:162	essential therapeutic options	134:162	essential therapeutic options for rare congenital lysosomal enzyme deficiencies	134:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	4	36	theme	hamster	661:667	arg1	cells					675:679	Chinese hamster ovary cells	653:679	Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	653:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	0	37	theme	recombinant	35:45	arg1	enzymes					69:75	recombinant lysosomal replacement enzymes	35:75	recombinant lysosomal replacement enzymes	35:75	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	1	38	theme	inefficient	311:321	arg1	biodistribution					323:337	inefficient biodistribution	311:337	inefficient biodistribution	311:337	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	6	39	theme	design	1191:1196	arg1	matrix					1198:1203	The developed design matrix	1177:1203	The developed design matrix	1177:1203	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	5	40	theme	glycoforms	904:913	arg1	time					864:867	distinct circulation time	843:867	distinct circulation time	843:867	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	40	theme	glycoforms	904:913	arg1	distribution					879:890	organ distribution	873:890	organ distribution	873:890	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	2	41	theme	glycan	421:426	arg1	structures					428:437	glycan structures	421:437	glycan structures	421:437	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
31040271	5	42	theme	hard-to-reach	1141:1153	arg1	heart					1170:1174	heart	1170:1174	heart	1170:1174	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	42	theme	hard-to-reach	1141:1153	arg1	organs					1155:1160	hard-to-reach organs	1141:1160	hard-to-reach organs such as heart	1141:1174	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	43	theme	mannose	1071:1077	arg1	receptors					1079:1087	the mannose 6-phosphate and mannose receptors	1043:1087	receptors	1079:1087	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	1	44	theme	clinical	230:237	arg1	use					239:241	clinical use	230:241	clinical use	230:241	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	0	45	theme	replacement	57:67	arg1	enzymes					69:75	recombinant lysosomal replacement enzymes	35:75	recombinant lysosomal replacement enzymes	35:75	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	5	46	theme	Fabry	941:945	arg1	model					961:965	a Fabry disease mouse model	939:965	a Fabry disease mouse model	939:965	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	47	theme	circulation	852:862	arg1	time					864:867	distinct circulation time	843:867	distinct circulation time	843:867	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	0	48	theme	lysosomal	47:55	arg1	enzymes					69:75	recombinant lysosomal replacement enzymes	35:75	recombinant lysosomal replacement enzymes	35:75	The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells.					
31040271	6	49	theme	systematic	1243:1252	arg1	studies					1254:1260	systematic studies	1243:1260	systematic studies	1243:1260	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	5	50	theme	improved	1098:1105	arg1	time					1119:1122	improved circulation time	1098:1122	improved circulation time	1098:1122	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	6	51	theme	engineered	1209:1218	arg1	lines					1229:1233	engineered CHO cell lines	1209:1233	engineered CHO cell lines	1209:1233	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	4	52	theme	key	767:769	arg1	features					778:785	key glycan features	767:785	key glycan features guiding cellular uptake and circulation	767:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	5	53	theme	circulation	1107:1117	arg1	time					1119:1122	improved circulation time	1098:1122	improved circulation time	1098:1122	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	6	54	theme	replacement	1287:1297	arg1	therapeutics					1299:1310	enzyme replacement therapeutics	1280:1310	enzyme replacement therapeutics	1280:1310	The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.					
31040271	1	55	from	enzymes	219:225	arg1	use					239:241	clinical use	230:241	clinical use	230:241	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	1	56	theme	essential	134:142	arg1	enzymes					122:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	1	56	theme	essential	134:142	arg1	options					156:162	essential therapeutic options	134:162	essential therapeutic options for rare congenital lysosomal enzyme deficiencies	134:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	3	57	theme	features	584:591	arg1	effects					556:562	effects	556:562	effects of different glycan features	556:591	It has not been possible to design and systematically study effects of different glycan features.					
31040271	5	58	dep	sialylated	990:999	arg1	α2-3					985:988	α2-3	985:988	α2-3	985:988	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	1	59	theme	therapeutic	144:154	arg1	enzymes					122:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes	100:128	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	1	59	theme	therapeutic	144:154	arg1	options					156:162	essential therapeutic options	134:162	essential therapeutic options for rare congenital lysosomal enzyme deficiencies	134:212	Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.					
31040271	4	60	theme	engineering	631:641	arg1	screen					643:648	a comprehensive gene engineering screen	610:648	a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	610:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	5	61	theme	disease	947:953	arg1	model					961:965	a Fabry disease mouse model	939:965	a Fabry disease mouse model	939:965	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	5	62	theme	mannose	1047:1053	arg1	6-phosphate					1055:1065	the mannose 6-phosphate and mannose receptors	1043:1087	6-phosphate	1055:1065	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	2	63	theme	glycan	399:404	arg1	receptors					406:414	cell surface glycan receptors	386:414	cell surface glycan receptors	386:414	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
31040271	5	64	theme	A	934:934	arg1	glycoforms					904:913	selected glycoforms	895:913	selected glycoforms of α-galactosidase A	895:934	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	4	65	theme	Chinese	653:659	arg1	cells					675:679	Chinese hamster ovary cells	653:679	Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation	653:825	Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation.					
31040271	5	66	theme	distinct	843:850	arg1	time					864:867	distinct circulation time	843:867	distinct circulation time	843:867	We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart.					
31040271	2	67	theme	surface	391:397	arg1	receptors					406:414	cell surface glycan receptors	386:414	cell surface glycan receptors	386:414	Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time.					
29665394	2	0	theme	further	457:463	arg1	process					475:481	the further isolation process	453:481	the further isolation process	453:481	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	2	1	with	%	446:446	arg1	process					475:481	the further isolation process	453:481	the further isolation process	453:481	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	1	2	theme	response	174:181	arg1	RSM					204:206	RSM	204:206	RSM	204:206	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	1	2	theme	response	174:181	arg1	methodology					191:201	response surface methodology	174:201	response surface methodology (RSM) method	174:214	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	4	3	theme	significant	918:928	arg1	activity					943:950	the significant anti-fatigue activity	914:950	the significant anti-fatigue activity	914:950	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	1	4	theme	surface	183:189	arg1	RSM					204:206	RSM	204:206	RSM	204:206	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	1	4	theme	surface	183:189	arg1	methodology					191:201	response surface methodology	174:201	response surface methodology (RSM) method	174:214	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	4	5	theme	urea	800:803	arg1	BUN					815:817	BUN	815:817	BUN	815:817	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	5	theme	urea	800:803	arg1	nitrogen					805:812	blood urea nitrogen	794:812	blood urea nitrogen (BUN)	794:818	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	5	theme	urea	800:803	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	2	6	theme	maca	294:297	arg1	yield					323:327	The maca polysaccharides extract yield	290:327	The maca polysaccharides extract yield of RSM	290:334	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	1	7	theme	optimal	121:127	arg1	conditions					140:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	4	8	theme	blood	794:798	arg1	BUN					815:817	BUN	815:817	BUN	815:817	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	8	theme	blood	794:798	arg1	nitrogen					805:812	blood urea nitrogen	794:812	blood urea nitrogen (BUN)	794:818	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	8	theme	blood	794:798	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	9	theme	anti-fatigue	685:696	arg1	activity					698:705	the anti-fatigue activity	681:705	the anti-fatigue activity	681:705	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	10	theme	polysaccharides	888:902	arg1	group					904:908	the low-dose maca polysaccharides group	870:908	the low-dose maca polysaccharides group	870:908	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	3	11	theme	2.34:10.21:1.00	651:665	arg1	ratio					642:646	the ratio	638:646	the ratio of 2.34:10.21:1.00	638:665	And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00.					
29665394	2	12	dep	model	371:375	arg1	predicts					377:384	predicts	377:384	predicts	377:384	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	4	13	theme	low-dose	874:881	arg1	group					904:908	the low-dose maca polysaccharides group	870:908	the low-dose maca polysaccharides group	870:908	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	14	theme	liver	773:777	arg1	LG					789:790	LG	789:790	LG	789:790	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	14	theme	liver	773:777	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	14	theme	liver	773:777	arg1	glycogen					779:786	liver glycogen	773:786	liver glycogen (LG)	773:791	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	15	contain	had	910:912	arg1	group					904:908	the low-dose maca polysaccharides group	870:908	the low-dose maca polysaccharides group	870:908	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	15	contain	had	910:912	arg2	activity					943:950	the significant anti-fatigue activity	914:950	the significant anti-fatigue activity	914:950	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	3	16	theme	monosaccharide	506:519	arg1	compositions					521:532	the monosaccharide compositions	502:532	the monosaccharide compositions obtained by gas chromatograph (GC)	502:567	And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00.					
29665394	0	17	theme	Maca	0:3	arg1	polysaccharides					5:19	Maca polysaccharides	0:19	Maca polysaccharides	0:19	Maca polysaccharides: Extraction optimization, structural features and anti-fatigue activities.					
29665394	3	18	theme	gas	546:548	arg1	GC					565:566	GC	565:566	GC	565:566	And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00.					
29665394	3	18	theme	gas	546:548	arg1	chromatograph					550:562	gas chromatograph	546:562	gas chromatograph (GC)	546:567	And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00.					
29665394	1	19	theme	maca	100:103	arg1	conditions					140:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	0	20	theme	Extraction	22:31	arg1	optimization					33:44	Extraction optimization	22:44	Extraction optimization	22:44	Maca polysaccharides: Extraction optimization, structural features and anti-fatigue activities.					
29665394	2	21	theme	extract	315:321	arg1	yield					323:327	The maca polysaccharides extract yield	290:327	The maca polysaccharides extract yield of RSM	290:334	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	2	22	theme	protein	411:417	arg1	purity					419:424	the total sugar and protein purity	391:424	purity	419:424	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	2	23	theme	polysaccharides	299:313	arg1	yield					323:327	The maca polysaccharides extract yield	290:327	The maca polysaccharides extract yield of RSM	290:334	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	4	24	dep	parameters	761:770	arg1	glycogen					779:786	liver glycogen	773:786	liver glycogen (LG)	773:791	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	acid					832:835	lactic acid	825:835	lactic acid (LD)	825:840	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	nitrogen					805:812	blood urea nitrogen	794:812	blood urea nitrogen (BUN)	794:818	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	LG					789:790	LG	789:790	LG	789:790	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	BUN					815:817	BUN	815:817	BUN	815:817	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	24	dep	parameters	761:770	arg1	LD					838:839	LD	838:839	LD	838:839	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	25	theme	biochemistry	748:759	arg1	nitrogen					805:812	blood urea nitrogen	794:812	blood urea nitrogen (BUN)	794:818	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	25	theme	biochemistry	748:759	arg1	parameter					737:745	the swimming parameter	724:745	the swimming parameter	724:745	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	25	theme	biochemistry	748:759	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	25	theme	biochemistry	748:759	arg1	glycogen					779:786	liver glycogen	773:786	liver glycogen (LG)	773:791	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	25	theme	biochemistry	748:759	arg1	acid					832:835	lactic acid	825:835	lactic acid (LD)	825:840	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	26	theme	lactic	825:830	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	26	theme	lactic	825:830	arg1	LD					838:839	LD	838:839	LD	838:839	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	26	theme	lactic	825:830	arg1	acid					832:835	lactic acid	825:835	lactic acid (LD)	825:840	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	1	27	theme	anti-fatigue	224:235	arg1	activity					237:244	the anti-fatigue activity	220:244	the anti-fatigue activity of maca polysaccharides (MCP)	220:274	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	4	28	theme	maca	883:886	arg1	group					904:908	the low-dose maca polysaccharides group	870:908	the low-dose maca polysaccharides group	870:908	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	1	29	dep	methodology	191:201	arg1	method					209:214	method	209:214	response surface methodology (RSM) method	174:214	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	1	30	theme	extraction	129:138	arg1	conditions					140:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	0	31	theme	structural	47:56	arg1	features					58:65	structural features	47:65	structural features	47:65	Maca polysaccharides: Extraction optimization, structural features and anti-fatigue activities.					
29665394	0	32	dep	optimization	33:44	arg1	polysaccharides					5:19	Maca polysaccharides	0:19	Maca polysaccharides	0:19	Maca polysaccharides: Extraction optimization, structural features and anti-fatigue activities.					
29665394	2	33	with	%	436:436	arg1	process					475:481	the further isolation process	453:481	the further isolation process	453:481	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	1	34	theme	polysaccharides	105:119	arg1	conditions					140:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions	96:149	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	2	35	theme	RSM	332:334	arg1	yield					323:327	The maca polysaccharides extract yield	290:327	The maca polysaccharides extract yield of RSM	290:334	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	0	36	theme	anti-fatigue	71:82	arg1	activities					84:93	anti-fatigue activities	71:93	anti-fatigue activities	71:93	Maca polysaccharides: Extraction optimization, structural features and anti-fatigue activities.					
29665394	2	37	theme	total	395:399	arg1	sugar					401:405	the total sugar and protein purity	391:424	sugar	401:405	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	2	37	theme	total	395:399	arg1	%					436:436	61.00%	431:436	61.00%	431:436	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	1	38	theme	maca	249:252	arg1	MCP					271:273	MCP	271:273	MCP	271:273	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	1	38	theme	maca	249:252	arg1	polysaccharides					254:268	maca polysaccharides	249:268	maca polysaccharides (MCP)	249:274	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
29665394	2	39	theme	isolation	465:473	arg1	process					475:481	the further isolation process	453:481	the further isolation process	453:481	The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively.					
29665394	4	40	theme	swimming	728:735	arg1	parameter					737:745	the swimming parameter	724:745	the swimming parameter	724:745	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	40	theme	swimming	728:735	arg1	parameters					761:770	biochemistry parameters	748:770	biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD))	748:841	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	4	41	theme	anti-fatigue	930:941	arg1	activity					943:950	the significant anti-fatigue activity	914:950	the significant anti-fatigue activity	914:950	Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.					
29665394	1	42	theme	polysaccharides	254:268	arg1	activity					237:244	the anti-fatigue activity	220:244	the anti-fatigue activity of maca polysaccharides (MCP)	220:274	The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored.					
30963354	8	0	theme	heparan	1112:1118	arg1	HS					1129:1130	HS	1129:1130	HS	1129:1130	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	0	theme	heparan	1112:1118	arg1	sulfate					1120:1126	heparan sulfate	1112:1126	heparan sulfate (HS)	1112:1131	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	12	1	theme	retina	1715:1720	arg1	profile					1692:1698	a distinctive GAG composition profile	1662:1698	a distinctive GAG composition profile of the axolotl retina	1662:1720	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	1	2	theme	blindness	232:240	arg1	worldwide					242:250	blindness worldwide	232:250	blindness worldwide	232:250	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	6	3	theme	decellularized	873:886	arg1	retina					908:913	native and decellularized axolotl and porcine retina	862:913	native and decellularized axolotl and porcine retina	862:913	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	12	4	theme	GAG	1676:1678	arg1	profile					1692:1698	a distinctive GAG composition profile	1662:1698	a distinctive GAG composition profile of the axolotl retina	1662:1720	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	5	5	theme	extracellular	650:662	arg1	ECM					672:674	ECM	672:674	ECM	672:674	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	5	5	theme	extracellular	650:662	arg1	matrix					664:669	extracellular matrix	650:669	extracellular matrix (ECM)	650:675	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	11	6	theme	HS	1461:1462	arg1	greater					1480:1486	greater	1480:1486	greater	1480:1486	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	11	6	theme	HS	1461:1462	arg1	composition					1464:1474	The HS composition	1457:1474	The HS composition	1457:1474	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	4	7	with	interaction	540:550	arg1	FGF					557:559	FGF	557:559	FGF	557:559	Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway.					
30963354	6	8	theme	native	862:867	arg1	retina					908:913	native and decellularized axolotl and porcine retina	862:913	native and decellularized axolotl and porcine retina	862:913	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	8	9	theme	native	1179:1184	arg1	axolotl					1205:1211	both native and decellularized axolotl	1174:1211	both native and decellularized axolotl	1174:1211	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	9	10	with	comparison	1299:1308	arg1	content					1350:1356	a higher content	1341:1356	a higher content of hyaluronic acid	1341:1375	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	9	10	with	comparison	1299:1308	arg1	retina					1323:1328	porcine retina	1315:1328	porcine retina	1315:1328	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	8	11	theme	hyaluronic	1134:1143	arg1	acid					1145:1148	hyaluronic acid	1134:1148	hyaluronic acid	1134:1148	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	12	theme	decellularized	1190:1203	arg1	axolotl					1205:1211	both native and decellularized axolotl	1174:1211	both native and decellularized axolotl	1174:1211	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	1	13	theme	retinitis	185:193	arg1	RP					207:208	RP	207:208	RP	207:208	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	13	theme	retinitis	185:193	arg1	pigmentosa					195:204	retinitis pigmentosa	185:204	retinitis pigmentosa (RP)	185:209	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	5	14	dep	amphibians	736:745	arg1	investigated					776:787	investigated	776:787	have not been investigated in the context of retinal regeneration	762:826	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	8	15	theme	keratan	1155:1161	arg1	sulfate					1163:1169	keratan sulfate	1155:1169	keratan sulfate	1155:1169	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	6	16	theme	mass	943:946	arg1	spectrometry					948:959	liquid chromatography mass spectrometry	921:959	liquid chromatography mass spectrometry	921:959	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	9	17	theme	acid	1372:1375	arg1	content					1350:1356	a higher content	1341:1356	a higher content of hyaluronic acid	1341:1375	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	5	18	theme	retinal	807:813	arg1	regeneration					815:826	retinal regeneration	807:826	retinal regeneration	807:826	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	6	19	theme	liquid	921:926	arg1	spectrometry					948:959	liquid chromatography mass spectrometry	921:959	liquid chromatography mass spectrometry	921:959	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	7	20	theme	mammalian	980:988	arg1	model					1001:1005	a mammalian vertebrate model	978:1005	a mammalian vertebrate model without the ability to regenerate retina	978:1046	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	7	20	theme	mammalian	980:988	arg1	Pig					962:964	Pig	962:964	Pig	962:964	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	2	21	dep	axolotl	295:301	arg1	mexicanum					314:322	Ambystoma mexicanum	304:322	Ambystoma mexicanum	304:322	Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood.					
30963354	10	22	located	observed	1429:1436	arg1	retina					1449:1454	axolotl retina	1441:1454	axolotl retina	1441:1454	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	10	22	located	observed	1429:1436	arg1	CS					1381:1382	CS	1381:1382	CS	1381:1382	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	10	22	located	observed	1429:1436	arg2	levels					1392:1397	higher levels	1385:1397	higher levels of 4- and 6- O-sulfation	1385:1422	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	1	23	theme	macular	154:160	arg1	AMD					176:178	AMD	176:178	AMD	176:178	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	23	theme	macular	154:160	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	7	24	used	used	970:973	arg2	Pig					962:964	Pig	962:964	Pig	962:964	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	7	24	used	used	970:973	arg2	model					1001:1005	a mammalian vertebrate model	978:1005	a mammalian vertebrate model without the ability to regenerate retina	978:1046	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	0	25	theme	Retina	95:100	arg1	analysis					33:40	Glycosaminoglycans compositional analysis	0:40	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.	0:101	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	2	26	theme	retinal	371:377	arg1	tissue					379:384	retinal tissue	371:384	retinal tissue	371:384	Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood.					
30963354	5	27	theme	lens	715:718	arg1	regeneration					720:731	lens regeneration	715:731	lens regeneration	715:731	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	9	28	theme	GAG	1272:1274	arg1	pattern					1288:1294	a distinctive GAG composition pattern	1258:1294	a distinctive GAG composition pattern	1258:1294	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	12	29	theme	axolotl	1804:1810	arg1	retina					1812:1817	the axolotl retina	1800:1817	the axolotl retina	1800:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	3	30	theme	Classic	408:414	arg1	pathways					426:433	Classic signaling pathways	408:433	Classic signaling pathways	408:433	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	3	30	theme	Classic	408:414	arg1	factor					464:469	fibroblast growth factor	446:469	fibroblast growth factor (FGF)	446:475	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	0	31	theme	Glycosaminoglycans	0:17	arg1	analysis					33:40	Glycosaminoglycans compositional analysis	0:40	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.	0:101	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	8	32	theme	Chondroitin	1049:1059	arg1	GAG					1095:1097	the main retinal GAG	1078:1097	the main retinal GAG	1078:1097	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	32	theme	Chondroitin	1049:1059	arg1	CS					1070:1071	CS	1070:1071	CS	1070:1071	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	32	theme	Chondroitin	1049:1059	arg1	sulfate					1061:1067	Chondroitin sulfate	1049:1067	Chondroitin sulfate (CS)	1049:1072	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	3	33	theme	growth	457:462	arg1	FGF					472:474	FGF	472:474	FGF	472:474	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	3	33	theme	growth	457:462	arg1	factor					464:469	fibroblast growth factor	446:469	fibroblast growth factor (FGF)	446:475	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	12	34	theme	regenerative	1773:1784	arg1	conditions					1786:1795	homeostatic and regenerative conditions	1757:1795	homeostatic and regenerative conditions of the axolotl retina	1757:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	1	35	theme	degenerative	111:122	arg1	diseases					124:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	35	theme	degenerative	111:122	arg1	causes					222:227	major causes	216:227	major causes of blindness worldwide	216:250	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	35	theme	degenerative	111:122	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	35	theme	degenerative	111:122	arg1	pigmentosa					195:204	retinitis pigmentosa	185:204	retinitis pigmentosa (RP)	185:209	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	9	36	theme	porcine	1315:1321	arg1	content					1350:1356	a higher content	1341:1356	a higher content of hyaluronic acid	1341:1375	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	9	36	theme	porcine	1315:1321	arg1	retina					1323:1328	porcine retina	1315:1328	porcine retina	1315:1328	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	11	37	theme	porcine	1534:1540	arg1	retina					1542:1547	porcine retina	1534:1547	porcine retina	1534:1547	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	12	38	theme	GAGs	1749:1752	arg1	role					1741:1744	role	1741:1744	role of GAGs in homeostatic and regenerative conditions of the axolotl retina	1741:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	12	39	from	role	1741:1744	arg1	conditions					1786:1795	homeostatic and regenerative conditions	1757:1795	homeostatic and regenerative conditions of the axolotl retina	1757:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	4	40	theme	Glycosaminoglycan	516:532	arg1	interaction					540:550	Glycosaminoglycan (GAG) interaction	516:550	Glycosaminoglycan (GAG) interaction with FGF	516:559	Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway.					
30963354	12	41	theme	homeostatic	1757:1767	arg1	conditions					1786:1795	homeostatic and regenerative conditions	1757:1795	homeostatic and regenerative conditions of the axolotl retina	1757:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	12	42	theme	axolotl	1707:1713	arg1	retina					1715:1720	the axolotl retina	1703:1720	the axolotl retina	1703:1720	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	4	43	theme	signal	577:582	arg1	transduction					584:595	signal transduction	577:595	signal transduction in this pathway	577:611	Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway.					
30963354	1	44	theme	major	216:220	arg1	diseases					124:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	44	theme	major	216:220	arg1	causes					222:227	major causes	216:227	major causes of blindness worldwide	216:250	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	44	theme	major	216:220	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	44	theme	major	216:220	arg1	pigmentosa					195:204	retinitis pigmentosa	185:204	retinitis pigmentosa (RP)	185:209	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	6	45	theme	porcine	900:906	arg1	retina					908:913	native and decellularized axolotl and porcine retina	862:913	native and decellularized axolotl and porcine retina	862:913	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	4	46	from	transduction	584:595	arg1	pathway					605:611	this pathway	600:611	this pathway	600:611	Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway.					
30963354	5	47	theme	anionic	623:629	arg1	GAGs					614:617	GAGs	614:617	GAGs	614:617	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	5	47	theme	anionic	623:629	arg1	polysaccharides					631:645	anionic polysaccharides	623:645	anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration	623:826	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	9	48	theme	hyaluronic	1361:1370	arg1	acid					1372:1375	hyaluronic acid	1361:1375	hyaluronic acid	1361:1375	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	6	49	theme	axolotl	888:894	arg1	retina					908:913	native and decellularized axolotl and porcine retina	862:913	native and decellularized axolotl and porcine retina	862:913	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	12	50	theme	composition	1680:1690	arg1	profile					1692:1698	a distinctive GAG composition profile	1662:1698	a distinctive GAG composition profile of the axolotl retina	1662:1720	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	1	51	theme	worldwide	242:250	arg1	diseases					124:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	51	theme	worldwide	242:250	arg1	causes					222:227	major causes	216:227	major causes of blindness worldwide	216:250	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	51	theme	worldwide	242:250	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	51	theme	worldwide	242:250	arg1	pigmentosa					195:204	retinitis pigmentosa	185:204	retinitis pigmentosa (RP)	185:209	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	11	52	theme	different	1586:1594	arg1	patterns					1606:1613	different sulfation patterns	1586:1613	different sulfation patterns	1586:1613	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	5	53	theme	regeneration	815:826	arg1	context					796:802	the context	792:802	the context of retinal regeneration	792:826	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	12	54	theme	distinctive	1664:1674	arg1	profile					1692:1698	a distinctive GAG composition profile	1662:1698	a distinctive GAG composition profile of the axolotl retina	1662:1720	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	11	55	theme	decellularized	1491:1504	arg1	tissues					1506:1512	decellularized tissues	1491:1512	decellularized tissues	1491:1512	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	12	56	theme	retinal	1856:1862	arg1	models					1877:1882	retinal regenerative models	1856:1882	retinal regenerative models	1856:1882	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	6	57	theme	chromatography	928:941	arg1	spectrometry					948:959	liquid chromatography mass spectrometry	921:959	liquid chromatography mass spectrometry	921:959	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	8	58	theme	porcine	1217:1223	arg1	retina					1225:1230	porcine retina	1217:1230	porcine retina	1217:1230	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	12	59	theme	models	1877:1882	arg1	understanding					1839:1851	our understanding	1835:1851	our understanding of retinal regenerative models	1835:1882	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	7	60	theme	vertebrate	990:999	arg1	model					1001:1005	a mammalian vertebrate model	978:1005	a mammalian vertebrate model without the ability to regenerate retina	978:1046	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	7	60	theme	vertebrate	990:999	arg1	Pig					962:964	Pig	962:964	Pig	962:964	Pig was used as a mammalian vertebrate model without the ability to regenerate retina.					
30963354	12	61	dep	suggest	1654:1660	arg1	set					1722:1724	set	1722:1724	set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina	1722:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	12	61	dep	suggest	1654:1660	arg1	understanding					1839:1851	our understanding	1835:1851	our understanding of retinal regenerative models	1835:1882	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	1	62	theme	age-related	142:152	arg1	AMD					176:178	AMD	176:178	AMD	176:178	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	62	theme	age-related	142:152	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	0	63	theme	Porcine	87:93	arg1	Retina					95:100	Porcine Retina	87:100	Porcine Retina	87:100	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	9	64	theme	Axolotl	1233:1239	arg1	retina					1241:1246	Axolotl retina	1233:1246	Axolotl retina	1233:1246	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	5	65	theme	amphibians	736:745	arg1	limb					706:709	limb	706:709	limb	706:709	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	5	65	theme	amphibians	736:745	arg1	regeneration					720:731	lens regeneration	715:731	lens regeneration	715:731	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	8	66	theme	retinal	1087:1093	arg1	sulfate					1061:1067	Chondroitin sulfate	1049:1067	Chondroitin sulfate (CS)	1049:1072	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	66	theme	retinal	1087:1093	arg1	GAG					1095:1097	the main retinal GAG	1078:1097	the main retinal GAG	1078:1097	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	67	from	GAG	1095:1097	arg1	axolotl					1205:1211	both native and decellularized axolotl	1174:1211	both native and decellularized axolotl	1174:1211	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	67	from	GAG	1095:1097	arg1	retina					1225:1230	porcine retina	1217:1230	porcine retina	1217:1230	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	10	68	theme	higher	1385:1390	arg1	levels					1392:1397	higher levels	1385:1397	higher levels of 4- and 6- O-sulfation	1385:1422	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	9	69	theme	distinctive	1260:1270	arg1	pattern					1288:1294	a distinctive GAG composition pattern	1258:1294	a distinctive GAG composition pattern	1258:1294	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	12	70	theme	retina	1812:1817	arg1	conditions					1786:1795	homeostatic and regenerative conditions	1757:1795	homeostatic and regenerative conditions of the axolotl retina	1757:1817	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	12	71	theme	regenerative	1864:1875	arg1	models					1877:1882	retinal regenerative models	1856:1882	retinal regenerative models	1856:1882	Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.					
30963354	0	72	theme	compositional	19:31	arg1	analysis					33:40	Glycosaminoglycans compositional analysis	0:40	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.	0:101	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	9	73	theme	composition	1276:1286	arg1	pattern					1288:1294	a distinctive GAG composition pattern	1258:1294	a distinctive GAG composition pattern	1258:1294	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	11	74	located	detected	1620:1627	arg1	axolotl					1632:1638	axolotl	1632:1638	axolotl	1632:1638	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	11	74	located	detected	1620:1627	arg2	patterns					1606:1613	different sulfation patterns	1586:1613	different sulfation patterns	1586:1613	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	3	75	theme	signaling	416:424	arg1	pathways					426:433	Classic signaling pathways	408:433	Classic signaling pathways	408:433	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	3	75	theme	signaling	416:424	arg1	factor					464:469	fibroblast growth factor	446:469	fibroblast growth factor (FGF)	446:475	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	1	76	theme	Retinal	103:109	arg1	diseases					124:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases	103:131	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	76	theme	Retinal	103:109	arg1	causes					222:227	major causes	216:227	major causes of blindness worldwide	216:250	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	76	theme	Retinal	103:109	arg1	degeneration					162:173	age-related macular degeneration	142:173	age-related macular degeneration (AMD)	142:179	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	1	76	theme	Retinal	103:109	arg1	pigmentosa					195:204	retinitis pigmentosa	185:204	retinitis pigmentosa (RP)	185:209	Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide.					
30963354	0	77	theme	axolotl	53:59	arg1	analysis					33:40	Glycosaminoglycans compositional analysis	0:40	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.	0:101	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	6	78	theme	GAG	829:831	arg1	composition					833:843	GAG composition	829:843	GAG composition	829:843	GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry.					
30963354	10	79	theme	O-sulfation	1412:1422	arg1	levels					1392:1397	higher levels	1385:1397	higher levels of 4- and 6- O-sulfation	1385:1422	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	11	80	theme	sulfation	1596:1604	arg1	patterns					1606:1613	different sulfation patterns	1586:1613	different sulfation patterns	1586:1613	The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl.					
30963354	3	81	theme	fibroblast	446:455	arg1	FGF					472:474	FGF	472:474	FGF	472:474	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	3	81	theme	fibroblast	446:455	arg1	factor					464:469	fibroblast growth factor	446:469	fibroblast growth factor (FGF)	446:475	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	0	82	theme	Ambystoma	62:70	arg1	axolotl					53:59	Urodele axolotl	45:59	Urodele axolotl (Ambystoma mexicanum)	45:81	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	0	82	theme	Ambystoma	62:70	arg1	mexicanum					72:80	Ambystoma mexicanum	62:80	Ambystoma mexicanum	62:80	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	8	83	theme	main	1082:1085	arg1	sulfate					1061:1067	Chondroitin sulfate	1049:1067	Chondroitin sulfate (CS)	1049:1072	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	8	83	theme	main	1082:1085	arg1	GAG					1095:1097	the main retinal GAG	1078:1097	the main retinal GAG	1078:1097	Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina.					
30963354	0	84	dep	axolotl	53:59	arg1	Urodele					45:51	Urodele	45:51	Urodele	45:51	Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.					
30963354	5	85	from	polysaccharides	631:645	arg1	ECM					672:674	ECM	672:674	ECM	672:674	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	5	85	from	polysaccharides	631:645	arg1	matrix					664:669	extracellular matrix	650:669	extracellular matrix (ECM)	650:675	GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration.					
30963354	10	86	theme	axolotl	1441:1447	arg1	retina					1449:1454	axolotl retina	1441:1454	axolotl retina	1441:1454	In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina.					
30963354	3	87	theme	axolotl	494:500	arg1	regeneration					502:513	axolotl regeneration	494:513	axolotl regeneration	494:513	Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration.					
30963354	9	88	theme	higher	1343:1348	arg1	content					1350:1356	a higher content	1341:1356	a higher content of hyaluronic acid	1341:1375	Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid.					
30963354	2	89	theme	laboratory-bred	328:342	arg1	axolotl					295:301	axolotl	295:301	axolotl (Ambystoma mexicanum)	295:323	Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood.					
30963354	2	89	theme	laboratory-bred	328:342	arg1	salamander					344:353	a laboratory-bred salamander	326:353	a laboratory-bred salamander	326:353	Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood.					
31130941	5	0	theme	C-terminal	1155:1164	arg1	hydrolysis					1144:1153	hydrolysis C-terminal	1144:1164	hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h	1144:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	15	1	theme	potential	2797:2805	arg1	tools					2830:2834	potential novel biotechnological tools	2797:2834	potential novel biotechnological tools	2797:2834	This further highlights the potential of B. bacteriovorus as a source to identify potential novel biotechnological tools.					
31130941	10	2	theme	diverse	2061:2067	arg1	functions					2069:2077	distinctive and diverse functions	2045:2077	distinctive and diverse functions in the immune system	2045:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	1	3	theme	inherent	308:315	arg1	plethora					317:324	their inherent plethora	302:324	their inherent plethora of hydrolytic enzymes	302:346	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	10	4	theme	vast	1998:2001	arg1	repertoire					2003:2012	a vast repertoire	1996:2012	a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system	1996:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	10	4	theme	vast	1998:2001	arg1	glycovariants					2026:2038	antibody glycovariants	2017:2038	antibody glycovariants with distinctive and diverse functions in the immune system	2017:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	13	5	theme	novel	2493:2497	arg1	tools					2499:2503	novel tools	2493:2503	novel tools	2493:2503	Such research has proven to be technically challenging and demonstrates a need for novel tools to facilitate such investigations.					
31130941	4	6	theme	proteins	872:879	arg1	Proteolysis					817:827	Proteolysis	817:827	Proteolysis of other native immunoglobulins and plasma proteins	817:879	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	10	7	from	functions	2069:2077	arg1	system					2093:2098	the immune system	2082:2098	the immune system	2082:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	10	8	with	glycovariants	2026:2038	arg1	functions					2069:2077	distinctive and diverse functions	2045:2077	distinctive and diverse functions in the immune system	2045:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	15	9	theme	bacteriovorus	2759:2771	arg1	potential					2743:2751	the potential	2739:2751	the potential of B. bacteriovorus	2739:2771	This further highlights the potential of B. bacteriovorus as a source to identify potential novel biotechnological tools.					
31130941	15	9	theme	bacteriovorus	2759:2771	arg1	source					2778:2783	a source	2776:2783	a source to identify potential novel biotechnological tools	2776:2834	This further highlights the potential of B. bacteriovorus as a source to identify potential novel biotechnological tools.					
31130941	10	10	theme	glycovariants	2026:2038	arg1	repertoire					2003:2012	a vast repertoire	1996:2012	a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system	1996:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	10	10	theme	glycovariants	2026:2038	arg1	glycovariants					2026:2038	antibody glycovariants	2017:2038	antibody glycovariants with distinctive and diverse functions in the immune system	2017:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	3	11	theme	secretory	767:775	arg1	SC					788:789	SC	788:789	SC	788:789	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	11	theme	secretory	767:775	arg1	component					777:785	the secretory component	763:785	the secretory component (SC) of secretory IgA (SIgA)	763:814	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	0	12	theme	IgA	150:152	arg1	Pattern					168:174	Site-Specific IgA Glycosylation Pattern	136:174	Site-Specific IgA Glycosylation Pattern	136:174	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	1	13	theme	biotechnological	420:435	arg1	tools					437:441	biotechnological tools	420:441	biotechnological tools	420:441	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	3	14	theme	preferential	620:631	arg1	sites					633:637	Mapping preferential sites	612:637	Mapping preferential sites of proteolytic activity	612:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	14	theme	preferential	620:631	arg1	activity					654:661	proteolytic activity	642:661	proteolytic activity	642:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	8	15	theme	Bdellovibrio	1615:1626	arg1	biology					1628:1634	Bdellovibrio biology	1615:1634	Bdellovibrio biology	1615:1634	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	7	16	theme	glycan	1505:1510	arg1	characterization					1512:1527	the specific glycan characterization	1492:1527	the specific glycan characterization of the released pIgA Fc-tail (Asn459)	1492:1565	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	3	17	theme	secretory	795:803	arg1	SIgA					810:813	SIgA	810:813	SIgA	810:813	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	17	theme	secretory	795:803	arg1	IgA					805:807	secretory IgA	795:807	secretory IgA (SIgA)	795:814	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	0	18	theme	Pattern	168:174	arg1	Elucidation					121:131	Elucidation	121:131	Elucidation of Site-Specific IgA Glycosylation Pattern	121:174	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	6	19	theme	enzymatic	1302:1310	arg1	activity					1312:1319	The enzymatic activity	1298:1319	The enzymatic activity	1298:1319	The enzymatic activity remained stable at pH 5.0-9.0 but was drastically reduced in the presence of MnCl2 and completely inhibited by ZnCl2.					
31130941	2	20	from	bacteriovorus	546:558	arg1	protease					495:502	a novel protease	487:502	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	9	21	theme	key	1828:1830	arg1	components					1832:1841	key components	1828:1841	key components of the immune system	1828:1862	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	3	22	theme	activity	654:661	arg1	sites					633:637	Mapping preferential sites	612:637	Mapping preferential sites of proteolytic activity	612:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	22	theme	activity	654:661	arg1	activity					654:661	proteolytic activity	642:661	proteolytic activity	642:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	10	23	theme	distinctive	2045:2055	arg1	functions					2069:2077	distinctive and diverse functions	2045:2077	distinctive and diverse functions in the immune system	2045:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	5	24	theme	R	1184:1184	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	4	25	theme	multiple	961:968	arg1	sites					979:983	multiple cleavage sites	961:983	multiple cleavage sites	961:983	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	15	26	theme	biotechnological	2813:2828	arg1	tools					2830:2834	potential novel biotechnological tools	2797:2834	potential novel biotechnological tools	2797:2834	This further highlights the potential of B. bacteriovorus as a source to identify potential novel biotechnological tools.					
31130941	9	27	theme	immune	1850:1855	arg1	system					1857:1862	the immune system	1846:1862	the immune system	1846:1862	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	5	28	theme	P	1187:1187	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	0	29	theme	Predatory	62:70	arg1	bacteriovorus					95:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	3	30	theme	proteolytic	679:689	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	30	theme	proteolytic	679:689	arg1	site					700:703	a single proteolytic cleavage site	670:703	a single proteolytic cleavage site of native plasma IgA (pIgA)	670:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	1	31	theme	increased	181:189	arg1	interest					191:198	The increased interest	177:198	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria	177:279	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	2	32	theme	Bdellovibrio	533:544	arg1	bacteriovorus					546:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	5	33	theme	S	1178:1178	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	0	34	theme	Bdellovibrio	82:93	arg1	bacteriovorus					95:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	2	35	dep	protease	495:502	arg1	protease					601:608	Bdellovibrio elastase-like serine protease	567:608	Bdellovibrio elastase-like serine protease	567:608	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	2	35	dep	protease	495:502	arg1	BspE					561:564	BspE	561:564	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	1	36	from	interest	191:198	arg1	bacteria					213:220	predatory bacteria	203:220	predatory bacteria	203:220	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	2	37	theme	elastase-like	580:592	arg1	protease					601:608	Bdellovibrio elastase-like serine protease	567:608	Bdellovibrio elastase-like serine protease	567:608	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	2	37	theme	elastase-like	580:592	arg1	BspE					561:564	BspE	561:564	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	5	38	theme	L	1181:1181	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	13	39	theme	such	2519:2522	arg1	investigations					2524:2537	such investigations	2519:2537	such investigations	2519:2537	Such research has proven to be technically challenging and demonstrates a need for novel tools to facilitate such investigations.					
31130941	1	40	theme	novel	391:395	arg1	agents					409:414	novel therapeutic agents	391:414	novel therapeutic agents	391:414	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	11	41	theme	available	2113:2121	arg1	information					2123:2133	the available information	2109:2133	the available information regarding antibody glycosylation	2109:2166	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	11	42	theme	antibody	2259:2266	arg1	treatments					2268:2277	therapeutic antibody treatments	2247:2277	therapeutic antibody treatments	2247:2277	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	5	43	theme	BspE	1200:1203	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	5	44	theme	Y	1169:1169	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	1	45	theme	agents	409:414	arg1	sources					380:386	natural sources	372:386	natural sources of novel therapeutic agents and biotechnological tools	372:441	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	8	46	theme	potential	1691:1699	arg1	tool					1701:1704	a potential tool	1689:1704	a potential tool to investigate the importance, and biological function of the pIgA Fc-tail	1689:1779	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	8	46	theme	potential	1691:1699	arg1	BspE					1667:1670	BspE	1667:1670	BspE	1667:1670	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	14	47	theme	IgA	2655:2657	arg1	study					2639:2643	the study	2635:2643	the study of plasma IgA	2635:2657	Here we have identified and characterized a novel protease from B. bacteriovorus, facilitating the study of plasma IgA by cleaving the Fc-tail, including the Asn459 N-glycan.					
31130941	2	48	theme	novel	489:493	arg1	protease					495:502	a novel protease	487:502	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	9	49	theme	glycosylation	1896:1908	arg1	importance					1873:1882	the importance	1869:1882	the importance of antibody glycosylation	1869:1908	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	5	50	theme	results	1221:1227	arg1	hydrolysis					1144:1153	hydrolysis C-terminal	1144:1164	hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h	1144:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	5	51	from	bonds	1065:1069	arg1	peptides					1082:1089	shorter peptides	1074:1089	shorter peptides	1074:1089	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	5	51	from	bonds	1065:1069	arg1	proteins					1105:1112	denatured proteins	1095:1112	denatured proteins	1095:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	13	52	theme	Such	2410:2413	arg1	research					2415:2422	Such research	2410:2422	Such research	2410:2422	Such research has proven to be technically challenging and demonstrates a need for novel tools to facilitate such investigations.					
31130941	7	53	theme	pIgA	1545:1548	arg1	Asn459					1559:1564	Asn459	1559:1564	Asn459	1559:1564	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	7	53	theme	pIgA	1545:1548	arg1	Fc-tail					1550:1556	the released pIgA Fc-tail	1532:1556	the released pIgA Fc-tail (Asn459)	1532:1565	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	5	54	theme	E	1190:1190	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	0	55	theme	Broad-Spectrum	8:21	arg1	Protease					44:51	A Novel Broad-Spectrum Elastase-Like Serine Protease	0:51	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus	0:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	1	56	theme	hydrolytic	329:338	arg1	enzymes					340:346	hydrolytic enzymes	329:346	hydrolytic enzymes	329:346	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	11	57	with	studies	2180:2186	arg1	IgG					2193:2195	IgG	2193:2195	IgG	2193:2195	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	2	58	theme	predatory	513:521	arg1	bacteriovorus					546:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	12	59	theme	glycosylation	2339:2351	arg1	Fc-structure					2361:2372	the Fc-structure	2357:2372	the Fc-structure for the remaining antibody classes	2357:2407	However, much is still unknown regarding the importance of glycosylation and the Fc-structure for the remaining antibody classes.					
31130941	12	59	theme	glycosylation	2339:2351	arg1	importance					2325:2334	the importance	2321:2334	the importance of glycosylation	2321:2351	However, much is still unknown regarding the importance of glycosylation and the Fc-structure for the remaining antibody classes.					
31130941	3	60	theme	IgA	722:724	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	60	theme	IgA	722:724	arg1	site					700:703	a single proteolytic cleavage site	670:703	a single proteolytic cleavage site of native plasma IgA (pIgA)	670:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	0	61	theme	Serine	37:42	arg1	Protease					44:51	A Novel Broad-Spectrum Elastase-Like Serine Protease	0:51	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus	0:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	5	62	theme	denatured	1095:1103	arg1	proteins					1105:1112	denatured proteins	1095:1112	denatured proteins	1095:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	4	63	dep	absent	892:897	arg1	4					995:995	4	995:995	4	995:995	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	albumin					917:923	albumin	917:923	albumin	917:923	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	IgG1					900:903	IgG1	900:903	IgG1	900:903	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	2					909:909	2	909:909	2	909:909	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	orosomucoid					929:939	orosomucoid	929:939	orosomucoid	929:939	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	IgG3					986:989	IgG3	986:989	IgG3	986:989	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	63	dep	absent	892:897	arg1	IgM					912:914	IgM	912:914	IgM	912:914	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	3	64	theme	native	708:713	arg1	pIgA					727:730	pIgA	727:730	pIgA	727:730	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	64	theme	native	708:713	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	4	65	theme	native	838:843	arg1	immunoglobulins					845:859	other native immunoglobulins	832:859	other native immunoglobulins	832:859	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	1	66	theme	antibiotic	251:260	arg1	bacteria					272:279	antibiotic resistant bacteria	251:279	antibiotic resistant bacteria	251:279	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	11	67	theme	antibody	2145:2152	arg1	glycosylation					2154:2166	antibody glycosylation	2145:2166	antibody glycosylation	2145:2166	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	5	68	theme	numerous	1232:1239	arg1	products					1250:1257	numerous cleavage products	1232:1257	numerous cleavage products sustaining activity for more than 6 h	1232:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	5	69	theme	shorter	1074:1080	arg1	peptides					1082:1089	shorter peptides	1074:1089	shorter peptides	1074:1089	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	10	70	theme	antibodies	1960:1969	arg1	Modifications					1943:1955	Modifications	1943:1955	Modifications of antibodies by glycosylation	1943:1986	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	4	71	dep	IgG3	986:989	arg1	IgE					998:1000	IgE	998:1000	IgE	998:1000	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	4	71	dep	IgG3	986:989	arg1	IgD					1003:1005	IgD	1003:1005	IgD	1003:1005	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	15	72	theme	novel	2807:2811	arg1	tools					2830:2834	potential novel biotechnological tools	2797:2834	potential novel biotechnological tools	2797:2834	This further highlights the potential of B. bacteriovorus as a source to identify potential novel biotechnological tools.					
31130941	12	73	theme	antibody	2392:2399	arg1	classes					2401:2407	the remaining antibody classes	2378:2407	the remaining antibody classes	2378:2407	However, much is still unknown regarding the importance of glycosylation and the Fc-structure for the remaining antibody classes.					
31130941	8	74	theme	biological	1741:1750	arg1	function					1752:1759	biological function	1741:1759	biological function	1741:1759	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	1	75	theme	predatory	203:211	arg1	bacteria					213:220	predatory bacteria	203:220	predatory bacteria	203:220	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	4	76	theme	immunoglobulins	845:859	arg1	Proteolysis					817:827	Proteolysis	817:827	Proteolysis of other native immunoglobulins and plasma proteins	817:879	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	2	77	theme	bacterium	523:531	arg1	bacteriovorus					546:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	the predatory bacterium Bdellovibrio bacteriovorus	509:558	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	11	78	dep	the	2232:2234	arg1	advance					2236:2242	advance	2236:2242	advance	2236:2242	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	6	79	theme	MnCl2	1398:1402	arg1	presence					1386:1393	the presence	1382:1393	the presence of MnCl2	1382:1402	The enzymatic activity remained stable at pH 5.0-9.0 but was drastically reduced in the presence of MnCl2 and completely inhibited by ZnCl2.					
31130941	4	80	theme	plasma	865:870	arg1	proteins					872:879	plasma proteins	865:879	plasma proteins	865:879	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	9	81	theme	IMPORTANCE	1782:1791	arg1	Antibodies					1793:1802	IMPORTANCE Antibodies	1782:1802	IMPORTANCE Antibodies	1782:1802	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	0	82	theme	Glycosylation	154:166	arg1	Pattern					168:174	Site-Specific IgA Glycosylation Pattern	136:174	Site-Specific IgA Glycosylation Pattern	136:174	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	3	83	theme	Mapping	612:618	arg1	sites					633:637	Mapping preferential sites	612:637	Mapping preferential sites of proteolytic activity	612:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	83	theme	Mapping	612:618	arg1	activity					654:661	proteolytic activity	642:661	proteolytic activity	642:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	8	84	theme	basic	1596:1600	arg1	knowledge					1602:1610	the basic knowledge	1592:1610	the basic knowledge of Bdellovibrio biology and proteases	1592:1648	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	7	85	theme	specific	1496:1503	arg1	characterization					1512:1527	the specific glycan characterization	1492:1527	the specific glycan characterization of the released pIgA Fc-tail (Asn459)	1492:1565	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	0	86	from	bacteriovorus	95:107	arg1	Protease					44:51	A Novel Broad-Spectrum Elastase-Like Serine Protease	0:51	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus	0:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	10	87	theme	antibody	2017:2024	arg1	glycovariants					2026:2038	antibody glycovariants	2017:2038	antibody glycovariants with distinctive and diverse functions in the immune system	2017:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	5	88	from	hydrolysis	1144:1153	arg1	products					1250:1257	numerous cleavage products	1232:1257	numerous cleavage products sustaining activity for more than 6 h	1232:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	1	89	theme	tools	437:441	arg1	sources					380:386	natural sources	372:386	natural sources of novel therapeutic agents and biotechnological tools	372:441	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	2	90	theme	serine	594:599	arg1	protease					601:608	Bdellovibrio elastase-like serine protease	567:608	Bdellovibrio elastase-like serine protease	567:608	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	2	90	theme	serine	594:599	arg1	BspE					561:564	BspE	561:564	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	14	91	theme	Asn459	2698:2703	arg1	N-glycan					2705:2712	the Asn459 N-glycan	2694:2712	the Asn459 N-glycan	2694:2712	Here we have identified and characterized a novel protease from B. bacteriovorus, facilitating the study of plasma IgA by cleaving the Fc-tail, including the Asn459 N-glycan.					
31130941	3	92	theme	IgA	805:807	arg1	SC					788:789	SC	788:789	SC	788:789	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	92	theme	IgA	805:807	arg1	component					777:785	the secretory component	763:785	the secretory component (SC) of secretory IgA (SIgA)	763:814	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	93	theme	proteolytic	642:652	arg1	activity					654:661	proteolytic activity	642:661	proteolytic activity	642:661	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	8	94	theme	biology	1628:1634	arg1	knowledge					1602:1610	the basic knowledge	1592:1610	the basic knowledge of Bdellovibrio biology and proteases	1592:1648	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	8	95	theme	pIgA	1768:1771	arg1	Fc-tail					1773:1779	the pIgA Fc-tail	1764:1779	the pIgA Fc-tail	1764:1779	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	7	96	used	utilized	1479:1486	arg2	hydrolysis					1443:1452	The hydrolysis	1439:1452	The hydrolysis of pIgA	1439:1460	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	3	97	theme	cleavage	691:698	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	97	theme	cleavage	691:698	arg1	site					700:703	a single proteolytic cleavage site	670:703	a single proteolytic cleavage site of native plasma IgA (pIgA)	670:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	8	98	theme	proteases	1640:1648	arg1	knowledge					1602:1610	the basic knowledge	1592:1610	the basic knowledge of Bdellovibrio biology and proteases	1592:1648	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	4	99	with	absent	892:897	arg1	sites					979:983	multiple cleavage sites	961:983	multiple cleavage sites	961:983	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	7	100	theme	pIgA	1457:1460	arg1	hydrolysis					1443:1452	The hydrolysis	1439:1452	The hydrolysis of pIgA	1439:1460	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	14	101	from	bacteriovorus	2607:2619	arg1	protease					2590:2597	a novel protease	2582:2597	a novel protease from B. bacteriovorus	2582:2619	Here we have identified and characterized a novel protease from B. bacteriovorus, facilitating the study of plasma IgA by cleaving the Fc-tail, including the Asn459 N-glycan.					
31130941	0	102	theme	Bacterium	72:80	arg1	bacteriovorus					95:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	the Predatory Bacterium Bdellovibrio bacteriovorus	58:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	3	103	theme	single	672:677	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	103	theme	single	672:677	arg1	site					700:703	a single proteolytic cleavage site	670:703	a single proteolytic cleavage site of native plasma IgA (pIgA)	670:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	9	104	theme	system	1857:1862	arg1	components					1832:1841	key components	1828:1841	key components of the immune system	1828:1862	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	8	105	dep	importance	1725:1734	arg1	the					1721:1723	the	1721:1723	the	1721:1723	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	1	106	theme	natural	372:378	arg1	sources					380:386	natural sources	372:386	natural sources of novel therapeutic agents and biotechnological tools	372:441	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	5	107	theme	most	1049:1052	arg1	bonds					1065:1069	most amino acid bonds	1049:1069	most amino acid bonds in shorter peptides and denatured proteins	1049:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	4	108	theme	cleavage	970:977	arg1	sites					979:983	multiple cleavage sites	961:983	multiple cleavage sites	961:983	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	2	109	theme	Bdellovibrio	567:578	arg1	protease					601:608	Bdellovibrio elastase-like serine protease	567:608	Bdellovibrio elastase-like serine protease	567:608	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	2	109	theme	Bdellovibrio	567:578	arg1	BspE					561:564	BspE	561:564	a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease)	487:609	Here, we have identified and characterized a novel protease from the predatory bacterium Bdellovibrio bacteriovorus: BspE (Bdellovibrio elastase-like serine protease).					
31130941	11	110	theme	therapeutic	2247:2257	arg1	treatments					2268:2277	therapeutic antibody treatments	2247:2277	therapeutic antibody treatments	2247:2277	Most of the available information regarding antibody glycosylation is based on studies with IgG, which have contributed greatly to the advance of therapeutic antibody treatments.					
31130941	8	111	used	used	1681:1684	arg2	BspE					1667:1670	BspE	1667:1670	BspE	1667:1670	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	8	111	used	used	1681:1684	arg2	tool					1701:1704	a potential tool	1689:1704	a potential tool to investigate the importance, and biological function of the pIgA Fc-tail	1689:1779	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	0	112	theme	Site-Specific	136:148	arg1	Pattern					168:174	Site-Specific IgA Glycosylation Pattern	136:174	Site-Specific IgA Glycosylation Pattern	136:174	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	1	113	theme	therapeutic	397:407	arg1	agents					409:414	novel therapeutic agents	391:414	novel therapeutic agents	391:414	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	10	114	theme	immune	2086:2091	arg1	system					2093:2098	the immune system	2082:2098	the immune system	2082:2098	Modifications of antibodies by glycosylation creates a vast repertoire of antibody glycovariants with distinctive and diverse functions in the immune system.					
31130941	9	115	theme	antibody	1887:1894	arg1	glycosylation					1896:1908	antibody glycosylation	1887:1908	antibody glycosylation	1887:1908	IMPORTANCE Antibodies are well-established as key components of the immune system, and the importance of antibody glycosylation is steadily gaining recognition.					
31130941	1	116	theme	resistant	262:270	arg1	bacteria					272:279	antibiotic resistant bacteria	251:279	antibiotic resistant bacteria	251:279	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	5	117	theme	autoproteolysis	1205:1219	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	0	118	theme	Novel	2:6	arg1	Protease					44:51	A Novel Broad-Spectrum Elastase-Like Serine Protease	0:51	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus	0:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	5	119	theme	amino	1054:1058	arg1	bonds					1065:1069	most amino acid bonds	1049:1069	most amino acid bonds in shorter peptides and denatured proteins	1049:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	14	120	theme	plasma	2648:2653	arg1	IgA					2655:2657	plasma IgA	2648:2657	plasma IgA	2648:2657	Here we have identified and characterized a novel protease from B. bacteriovorus, facilitating the study of plasma IgA by cleaving the Fc-tail, including the Asn459 N-glycan.					
31130941	12	121	theme	remaining	2382:2390	arg1	classes					2401:2407	the remaining antibody classes	2378:2407	the remaining antibody classes	2378:2407	However, much is still unknown regarding the importance of glycosylation and the Fc-structure for the remaining antibody classes.					
31130941	5	122	theme	broad	1026:1030	arg1	activity					1032:1039	a broad activity	1024:1039	a broad activity against most amino acid bonds in shorter peptides and denatured proteins	1024:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	0	123	theme	Elastase-Like	23:35	arg1	Protease					44:51	A Novel Broad-Spectrum Elastase-Like Serine Protease	0:51	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus	0:107	A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory Bacterium Bdellovibrio bacteriovorus Facilitates Elucidation of Site-Specific IgA Glycosylation Pattern.					
31130941	5	124	theme	slight	1122:1127	arg1	preference					1129:1138	a slight preference	1120:1138	a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h	1120:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	7	125	theme	Fc-tail	1550:1556	arg1	characterization					1512:1527	the specific glycan characterization	1492:1527	the specific glycan characterization of the released pIgA Fc-tail (Asn459)	1492:1565	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	3	126	theme	plasma	715:720	arg1	pIgA					727:730	pIgA	727:730	pIgA	727:730	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	3	126	theme	plasma	715:720	arg1	IgA					722:724	native plasma IgA	708:724	native plasma IgA (pIgA)	708:731	Mapping preferential sites of proteolytic activity showed a single proteolytic cleavage site of native plasma IgA (pIgA) in the Fc-tail; as well as in the secretory component (SC) of secretory IgA (SIgA).					
31130941	1	127	theme	enzymes	340:346	arg1	plethora					317:324	their inherent plethora	302:324	their inherent plethora of hydrolytic enzymes	302:346	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	1	127	theme	enzymes	340:346	arg1	potential					359:367	their potential	353:367	their potential as natural sources of novel therapeutic agents and biotechnological tools	353:441	The increased interest in predatory bacteria due to their ability to kill antibiotic resistant bacteria has also highlighted their inherent plethora of hydrolytic enzymes, and their potential as natural sources of novel therapeutic agents and biotechnological tools.					
31130941	7	128	theme	released	1536:1543	arg1	Asn459					1559:1564	Asn459	1559:1564	Asn459	1559:1564	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	7	128	theme	released	1536:1543	arg1	Fc-tail					1550:1556	the released pIgA Fc-tail	1532:1556	the released pIgA Fc-tail (Asn459)	1532:1565	The hydrolysis of pIgA was subsequently utilized for the specific glycan characterization of the released pIgA Fc-tail (Asn459).					
31130941	5	129	theme	acid	1060:1063	arg1	bonds					1065:1069	most amino acid bonds	1049:1069	most amino acid bonds in shorter peptides and denatured proteins	1049:1112	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	8	130	theme	Fc-tail	1773:1779	arg1	importance					1725:1734	importance	1725:1734	importance	1725:1734	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	8	130	theme	Fc-tail	1773:1779	arg1	function					1752:1759	biological function	1741:1759	biological function	1741:1759	Besides contributing to the basic knowledge of Bdellovibrio biology and proteases, we propose that BspE could be used as a potential tool to investigate the importance, and biological function of the pIgA Fc-tail.					
31130941	4	131	theme	other	832:836	arg1	immunoglobulins					845:859	other native immunoglobulins	832:859	other native immunoglobulins	832:859	Proteolysis of other native immunoglobulins and plasma proteins was either absent (IgG1 and 2, IgM, albumin and orosomucoid) or unspecific with multiple cleavage sites (IgG3 and 4, IgE, IgD).					
31130941	5	132	theme	V	1172:1172	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	14	133	theme	novel	2584:2588	arg1	protease					2590:2597	a novel protease	2582:2597	a novel protease from B. bacteriovorus	2582:2619	Here we have identified and characterized a novel protease from B. bacteriovorus, facilitating the study of plasma IgA by cleaving the Fc-tail, including the Asn459 N-glycan.					
31130941	5	134	theme	cleavage	1241:1248	arg1	products					1250:1257	numerous cleavage products	1232:1257	numerous cleavage products sustaining activity for more than 6 h	1232:1295	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31130941	5	135	theme	F	1175:1175	arg1	results					1221:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results	1169:1227	BspE displayed a broad activity against most amino acid bonds in shorter peptides and denatured proteins, with a slight preference for hydrolysis C-terminal of Y, V, F, S, L, R, P, E, and K. BspE autoproteolysis results in numerous cleavage products sustaining activity for more than 6 h.					
31601857	6	0	theme	overall	1235:1241	arg1	rate					1252:1255	the overall survival rate	1231:1255	the overall survival rate	1231:1255	Accordingly, the overall survival rate was found to significantly correlate with the fAGP level.					
31601857	4	1	contain	had	771:773	arg2	levels					799:804	significantly high fAGP levels	775:804	significantly high fAGP levels above the cut-off value (H-fAGP)	775:837	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	4	1	contain	had	771:773	arg1	patients					762:769	Twenty-three patients	749:769	Twenty-three patients	749:769	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	4	2	theme	Twenty-three	749:760	arg1	patients					762:769	Twenty-three patients	749:769	Twenty-three patients	749:769	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	0	3	theme	tumor	79:83	arg1	immunotherapy					85:97	tumor immunotherapy	79:97	tumor immunotherapy of patients with lung cancer	79:126	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	5	4	theme	cut-off	1096:1102	arg1	L-fAGP					1111:1116	L-fAGP	1111:1116	L-fAGP	1111:1116	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	5	4	theme	cut-off	1096:1102	arg1	value					1104:1108	the cut-off value	1092:1108	the cut-off value (L-fAGP)	1092:1117	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	2	5	gly	α1,3fucosylated	455:469	arg1	fAGP					476:479	fAGP	476:479	fAGP	476:479	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	5	gly	α1,3fucosylated	455:469	arg1	AGP					471:473	α1,3fucosylated AGP	455:473	α1,3fucosylated AGP (fAGP) levels	455:487	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	6	theme	glycoprotein	387:398	arg1	structures					359:368	glycan structures	352:368	glycan structures of serum α1-acid glycoprotein (AGP)	352:404	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	0	7	theme	patients	102:109	arg1	immunotherapy					85:97	tumor immunotherapy	79:97	tumor immunotherapy of patients with lung cancer	79:126	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	5	8	theme	2-year	1138:1143	arg1	observation					1145:1155	a 2-year observation even though 5 patients in this group had no tumor shrinkage	1136:1215	a 2-year observation even though 5 patients in this group had no tumor shrinkage	1136:1215	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	1	9	theme	treatment	294:302	arg1	outcomes					304:311	treatment outcomes	294:311	treatment outcomes	294:311	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	2	10	theme	chemotherapy	558:569	arg1	treatments					571:580	chemotherapy treatments	558:580	chemotherapy treatments	558:580	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	4	11	theme	cut-off	816:822	arg1	value					824:828	the cut-off value	812:828	the cut-off value (H-fAGP)	812:837	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	4	11	theme	cut-off	816:822	arg1	H-fAGP					831:836	H-fAGP	831:836	H-fAGP	831:836	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	0	12	theme	lung	116:119	arg1	cancer					121:126	lung cancer	116:126	lung cancer	116:126	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	4	13	from	patients	888:895	arg1	group					905:909	this group	900:909	this group	900:909	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	2	14	theme	cancer	430:435	arg1	patients					437:444	cancer patients	430:444	cancer patients	430:444	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	4	15	theme	high	959:962	arg1	levels					969:974	high fAGP levels	959:974	high fAGP levels	959:974	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	1	16	theme	remarkable	194:203	arg1	benefits					214:221	remarkable clinical benefits	194:221	remarkable clinical benefits in cancer patients	194:240	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	0	17	theme	α1-acid	12:18	arg1	glycoprotein					20:31	Fucosylated α1-acid glycoprotein	0:31	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis	0:67	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	2	18	theme	disease	510:516	arg1	progression					518:528	disease progression	510:528	disease progression	510:528	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	5	19	from	patients	1171:1178	arg1	group					1188:1192	this group	1183:1192	this group	1183:1192	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	2	20	theme	glycan	352:357	arg1	structures					359:368	glycan structures	352:368	glycan structures of serum α1-acid glycoprotein (AGP)	352:404	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	6	21	theme	fAGP	1303:1306	arg1	level					1308:1312	the fAGP level	1299:1312	the fAGP level	1299:1312	Accordingly, the overall survival rate was found to significantly correlate with the fAGP level.					
31601857	1	22	theme	clinical	205:212	arg1	benefits					214:221	remarkable clinical benefits	194:221	remarkable clinical benefits in cancer patients	194:240	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	0	23	theme	Fucosylated	0:10	arg1	glycoprotein					20:31	Fucosylated α1-acid glycoprotein	0:31	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis	0:67	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	5	24	contain	had	1194:1196	arg2	shrinkage					1207:1215	no tumor shrinkage	1198:1215	no tumor shrinkage	1198:1215	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	5	24	contain	had	1194:1196	arg1	patients					1171:1178	5 patients	1169:1178	5 patients in this group	1169:1192	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	0	25	with	patients	102:109	arg1	cancer					121:126	lung cancer	116:126	lung cancer	116:126	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	4	26	theme	fAGP	794:797	arg1	levels					799:804	significantly high fAGP levels	775:804	significantly high fAGP levels above the cut-off value (H-fAGP)	775:837	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	7	27	theme	Multivariate	1315:1326	arg1	analyses					1328:1335	Multivariate analyses	1315:1335	Multivariate analyses	1315:1335	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	8	28	theme	fAGP	1432:1435	arg1	level					1437:1441	the fAGP level	1428:1441	the fAGP level	1428:1441	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	8	28	theme	fAGP	1432:1435	arg1	biomarker					1469:1477	a reliable biomarker	1458:1477	a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab	1458:1542	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	7	29	theme	independent	1369:1379	arg1	H-fAGP					1355:1360	the H-fAGP	1351:1360	the H-fAGP	1351:1360	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	7	29	theme	independent	1369:1379	arg1	factor					1386:1391	an independent risk factor	1366:1391	an independent risk factor for cancer progression	1366:1414	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	2	30	theme	α1,3fucosylated	455:469	arg1	fAGP					476:479	fAGP	476:479	fAGP	476:479	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	30	theme	α1,3fucosylated	455:469	arg1	AGP					471:473	α1,3fucosylated AGP	455:473	α1,3fucosylated AGP (fAGP) levels	455:487	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	31	theme	AGP	471:473	arg1	levels					482:487	α1,3fucosylated AGP (fAGP) levels	455:487	α1,3fucosylated AGP (fAGP) levels	455:487	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	8	32	theme	immunotherapy	1515:1527	arg1	efficacy					1503:1510	clinical efficacy	1494:1510	clinical efficacy of immunotherapy with nivolumab	1494:1542	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	7	33	theme	cancer	1397:1402	arg1	progression					1404:1414	cancer progression	1397:1414	cancer progression	1397:1414	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	1	34	theme	cancer	226:231	arg1	patients					233:240	cancer patients	226:240	cancer patients	226:240	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	2	35	gly	glycoprotein	387:398	arg1	AGP					401:403	AGP	401:403	AGP	401:403	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	35	gly	glycoprotein	387:398	arg1	glycoprotein					387:398	serum α1-acid glycoprotein	373:398	serum α1-acid glycoprotein (AGP)	373:404	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	7	36	theme	risk	1381:1384	arg1	H-fAGP					1355:1360	the H-fAGP	1351:1360	the H-fAGP	1351:1360	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	7	36	theme	risk	1381:1384	arg1	factor					1386:1391	an independent risk factor	1366:1391	an independent risk factor for cancer progression	1366:1414	Multivariate analyses revealed that the H-fAGP was an independent risk factor for cancer progression.					
31601857	3	37	theme	lung	673:676	arg1	cancer					678:683	advanced lung cancer	664:683	advanced lung cancer	664:683	Here, the fAGP was analyzed in sera prospectively obtained from 39 patients with advanced lung cancer who underwent immunotherapy with anti-PD-1 antibody, nivolumab.					
31601857	8	38	theme	reliable	1460:1467	arg1	level					1437:1441	the fAGP level	1428:1441	the fAGP level	1428:1441	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	8	38	theme	reliable	1460:1467	arg1	biomarker					1469:1477	a reliable biomarker	1458:1477	a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab	1458:1542	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	0	39	gly	glycoprotein	20:31	arg1	glycoprotein					20:31	Fucosylated α1-acid glycoprotein	0:31	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis	0:67	Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer.					
31601857	2	40	theme	α1-acid	379:385	arg1	AGP					401:403	AGP	401:403	AGP	401:403	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	40	theme	α1-acid	379:385	arg1	glycoprotein					387:398	serum α1-acid glycoprotein	373:398	serum α1-acid glycoprotein (AGP)	373:404	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	4	41	theme	tumor	918:922	arg1	sizes					924:928	sizes	924:928	sizes	924:928	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	3	42	theme	anti-PD-1	718:726	arg1	nivolumab					738:746	nivolumab	738:746	nivolumab	738:746	Here, the fAGP was analyzed in sera prospectively obtained from 39 patients with advanced lung cancer who underwent immunotherapy with anti-PD-1 antibody, nivolumab.					
31601857	3	42	theme	anti-PD-1	718:726	arg1	antibody					728:735	anti-PD-1 antibody	718:735	anti-PD-1 antibody	718:735	Here, the fAGP was analyzed in sera prospectively obtained from 39 patients with advanced lung cancer who underwent immunotherapy with anti-PD-1 antibody, nivolumab.					
31601857	5	43	theme	fAGP	1050:1053	arg1	levels					1055:1060	levels	1055:1060	levels	1055:1060	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	2	44	theme	serum	373:377	arg1	AGP					401:403	AGP	401:403	AGP	401:403	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	2	44	theme	serum	373:377	arg1	glycoprotein					387:398	serum α1-acid glycoprotein	373:398	serum α1-acid glycoprotein (AGP)	373:404	Recently, we demonstrated that glycan structures of serum α1-acid glycoprotein (AGP) changed dramatically in cancer patients and that α1,3fucosylated AGP (fAGP) levels increased along with disease progression and decreased responding to chemotherapy treatments.					
31601857	5	45	theme	tumor	1201:1205	arg1	shrinkage					1207:1215	no tumor shrinkage	1198:1215	no tumor shrinkage	1198:1215	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	6	46	theme	survival	1243:1250	arg1	rate					1252:1255	the overall survival rate	1231:1255	the overall survival rate	1231:1255	Accordingly, the overall survival rate was found to significantly correlate with the fAGP level.					
31601857	4	47	theme	high	789:792	arg1	levels					799:804	significantly high fAGP levels	775:804	significantly high fAGP levels above the cut-off value (H-fAGP)	775:837	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
31601857	3	48	theme	advanced	664:671	arg1	cancer					678:683	advanced lung cancer	664:683	advanced lung cancer	664:683	Here, the fAGP was analyzed in sera prospectively obtained from 39 patients with advanced lung cancer who underwent immunotherapy with anti-PD-1 antibody, nivolumab.					
31601857	5	49	theme	other	1025:1029	arg1	patients					1034:1041	the other 16 patients	1021:1041	the other 16 patients	1021:1041	However, the other 16 patients, whose fAGP levels decreased or maintained below the cut-off value (L-fAGP), survived during a 2-year observation even though 5 patients in this group had no tumor shrinkage.					
31601857	1	50	from	benefits	214:221	arg1	patients					233:240	cancer patients	226:240	cancer patients	226:240	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	1	51	theme	immune	153:158	arg1	molecules					171:179	immune checkpoint molecules	153:179	immune checkpoint molecules	153:179	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	8	52	with	efficacy	1503:1510	arg1	nivolumab					1534:1542	nivolumab	1534:1542	nivolumab	1534:1542	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	1	53	theme	relevant	260:267	arg1	biomarker					269:277	no clinically relevant biomarker	246:277	no clinically relevant biomarker for predicting treatment outcomes	246:311	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	3	54	with	patients	650:657	arg1	cancer					678:683	advanced lung cancer	664:683	advanced lung cancer	664:683	Here, the fAGP was analyzed in sera prospectively obtained from 39 patients with advanced lung cancer who underwent immunotherapy with anti-PD-1 antibody, nivolumab.					
31601857	8	55	theme	clinical	1494:1501	arg1	efficacy					1503:1510	clinical efficacy	1494:1510	clinical efficacy of immunotherapy with nivolumab	1494:1542	Therefore, the fAGP level appeared to be a reliable biomarker for predicting clinical efficacy of immunotherapy with nivolumab.					
31601857	1	56	theme	checkpoint	160:169	arg1	molecules					171:179	immune checkpoint molecules	153:179	immune checkpoint molecules	153:179	Immunotherapy targeting immune checkpoint molecules has provided remarkable clinical benefits in cancer patients but no clinically relevant biomarker for predicting treatment outcomes exists.					
31601857	4	57	theme	fAGP	964:967	arg1	levels					969:974	high fAGP levels	959:974	high fAGP levels	959:974	Twenty-three patients had significantly high fAGP levels above the cut-off value (H-fAGP) at one month after starting the treatment and 20 patients in this group, whose tumor sizes did not decrease, maintained high fAGP levels continuously and subsequently died.					
30884721	0	0	theme	agents	86:91	arg1	release					61:67	pH controlled release	47:67	pH controlled release of antiglycation agents	47:91	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	10	1	theme	antiglycation	1397:1409	arg1	activity					1411:1418	antiglycation activity	1397:1418	antiglycation activity	1397:1418	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	3	2	theme	calcium	470:476	arg1	chloride					478:485	calcium chloride	470:485	calcium chloride	470:485	Microbeads were obtained by a vibrating nozzle method using calcium chloride as hardening agent.					
30884721	1	3	theme	leading	223:229	arg1	cause					231:235	the leading cause	219:235	the leading cause of hyperglycaemia damage	219:260	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	3	theme	leading	223:229	arg1	sugars					178:183	reducing sugars	169:183	reducing sugars	169:183	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	3	theme	leading	223:229	arg1	compounds					199:207	dicarbonyl compounds	188:207	dicarbonyl compounds	188:207	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	0	4	theme	antiglycation	72:84	arg1	agents					86:91	antiglycation agents	72:91	antiglycation agents	72:91	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	6	5	theme	flavonol	829:836	arg1	release					838:844	flavonol release	829:844	flavonol release	829:844	The percent release depended on the compound: procyanidin B1 release was 74%, catechin and epicatechin release was ~ 50%, while anthocyanin and flavonol release was less than 11%.					
30884721	2	6	theme	alginate	319:326	arg1	hydrogel					328:335	alginate hydrogel	319:335	alginate hydrogel	319:335	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	11	7	theme	diseases	1660:1667	arg1	prevention					1607:1616	the prevention	1603:1616	the prevention of advanced-glycation-endproducts related diseases	1603:1667	This functionalized polymer could be applied in the prevention of advanced-glycation-endproducts related diseases.					
30884721	6	8	theme	procyanidin	731:741	arg1	release					746:752	procyanidin B1 release	731:752	procyanidin B1 release	731:752	The percent release depended on the compound: procyanidin B1 release was 74%, catechin and epicatechin release was ~ 50%, while anthocyanin and flavonol release was less than 11%.					
30884721	8	9	from	CE/kg	1175:1179	arg1	system					1226:1231	the methylglyoxal model system	1202:1231	the methylglyoxal model system	1202:1231	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	9	from	CE/kg	1175:1179	arg1	system					1156:1161	the fructose model system	1137:1161	the fructose model system	1137:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	10	theme	model	1220:1224	arg1	system					1226:1231	the methylglyoxal model system	1202:1231	the methylglyoxal model system	1202:1231	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	11	theme	antiglycation	1051:1063	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	11	theme	antiglycation	1051:1063	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	12	theme	methylglyoxal	1206:1218	arg1	system					1226:1231	the methylglyoxal model system	1202:1231	the methylglyoxal model system	1202:1231	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	1	13	theme	hyperglycaemia	240:253	arg1	damage					255:260	hyperglycaemia damage	240:260	hyperglycaemia damage	240:260	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	8	14	theme	246	1078:1080	arg1	mmol					1082:1085	mmol	1082:1085	mmol	1082:1085	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	15	theme	mmol	1170:1173	arg1	CE/kg					1175:1179	78 mmol CE/kg	1167:1179	78 mmol CE/kg of dry microbeads	1167:1197	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	5	16	theme	alginate	576:583	arg1	microbeads					585:594	the alginate microbeads	572:594	the alginate microbeads	572:594	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	2	17	used	used	341:344	arg2	hydrogel					328:335	alginate hydrogel	319:335	alginate hydrogel	319:335	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	8	18	theme	equivalents	1096:1106	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	18	theme	equivalents	1096:1106	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	1	19	theme	proteins	153:160	arg1	damage					143:148	structural damage	132:148	structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases	132:301	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	9	20	theme	expected	1282:1289	arg1	activity					1291:1298	the expected activity	1278:1298	the expected activity	1278:1298	These values corresponded to 68% and 62% of the expected activity, probably due to interaction between phenolics and the alginate carrier.					
30884721	11	21	theme	related	1652:1658	arg1	diseases					1660:1667	advanced-glycation-endproducts related diseases	1621:1667	advanced-glycation-endproducts related diseases	1621:1667	This functionalized polymer could be applied in the prevention of advanced-glycation-endproducts related diseases.					
30884721	6	22	theme	B1	743:744	arg1	release					746:752	procyanidin B1 release	731:752	procyanidin B1 release	731:752	The percent release depended on the compound: procyanidin B1 release was 74%, catechin and epicatechin release was ~ 50%, while anthocyanin and flavonol release was less than 11%.					
30884721	10	23	theme	GS	1541:1542	arg1	phenolics					1544:1552	GS phenolics	1541:1552	GS phenolics	1541:1552	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	2	24	theme	GS	361:362	arg1	phenolics					364:372	GS phenolics	361:372	GS phenolics	361:372	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	2	24	theme	GS	361:362	arg1	system					402:407	a pH dependent releasing system	377:407	a pH dependent releasing system	377:407	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	8	25	theme	catechin	1087:1094	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	25	theme	catechin	1087:1094	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	3	26	theme	hardening	490:498	arg1	agent					500:504	hardening agent	490:504	hardening agent	490:504	Microbeads were obtained by a vibrating nozzle method using calcium chloride as hardening agent.					
30884721	7	27	theme	serum	974:978	arg1	albumin					980:986	bovine serum albumin	967:986	bovine serum albumin	967:986	At pH 7.4, the microbeads were dissolved and formed a viscous solution that showed ability to protect bovine serum albumin from glycation induced by both fructose and methylglyoxal.					
30884721	4	28	theme	total	536:540	arg1	phenolics					542:550	total phenolics	536:550	total phenolics	536:550	Encapsulation efficiency for total phenolics was 68%.					
30884721	8	29	from	/kg	1112:1114	arg1	system					1226:1231	the methylglyoxal model system	1202:1231	the methylglyoxal model system	1202:1231	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	29	from	/kg	1112:1114	arg1	system					1156:1161	the fructose model system	1137:1161	the fructose model system	1137:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	30	theme	mmol	1082:1085	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	30	theme	mmol	1082:1085	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	1	31	theme	damage	255:260	arg1	cause					231:235	the leading cause	219:235	the leading cause of hyperglycaemia damage	219:260	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	31	theme	damage	255:260	arg1	sugars					178:183	reducing sugars	169:183	reducing sugars	169:183	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	31	theme	damage	255:260	arg1	compounds					199:207	dicarbonyl compounds	188:207	dicarbonyl compounds	188:207	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	8	32	theme	microbeads	1123:1132	arg1	CE/kg					1175:1179	78 mmol CE/kg	1167:1179	78 mmol CE/kg of dry microbeads	1167:1197	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	32	theme	microbeads	1123:1132	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	32	theme	microbeads	1123:1132	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	1	33	theme	Grape	94:98	arg1	phenolics					110:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	34	theme	reducing	169:176	arg1	cause					231:235	the leading cause	219:235	the leading cause of hyperglycaemia damage	219:260	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	34	theme	reducing	169:176	arg1	sugars					178:183	reducing sugars	169:183	reducing sugars	169:183	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	34	theme	reducing	169:176	arg1	compounds					199:207	dicarbonyl compounds	188:207	dicarbonyl compounds	188:207	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	0	35	theme	skin	28:31	arg1	phenolics					33:41	grape skin phenolics	22:41	grape skin phenolics	22:41	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	9	36	theme	activity	1291:1298	arg1	activity					1291:1298	the expected activity	1278:1298	the expected activity	1278:1298	These values corresponded to 68% and 62% of the expected activity, probably due to interaction between phenolics and the alginate carrier.					
30884721	9	36	theme	activity	1291:1298	arg1	%					1265:1265	68%	1263:1265	68%	1263:1265	These values corresponded to 68% and 62% of the expected activity, probably due to interaction between phenolics and the alginate carrier.					
30884721	9	36	theme	activity	1291:1298	arg1	%					1273:1273	62%	1271:1273	62%	1271:1273	These values corresponded to 68% and 62% of the expected activity, probably due to interaction between phenolics and the alginate carrier.					
30884721	1	37	theme	skin	100:103	arg1	phenolics					110:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	2	38	theme	dependent	382:390	arg1	phenolics					364:372	GS phenolics	361:372	GS phenolics	361:372	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	2	38	theme	dependent	382:390	arg1	system					402:407	a pH dependent releasing system	377:407	a pH dependent releasing system	377:407	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	0	39	theme	grape	22:26	arg1	phenolics					33:41	grape skin phenolics	22:41	grape skin phenolics	22:41	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	7	40	theme	bovine	967:972	arg1	albumin					980:986	bovine serum albumin	967:986	bovine serum albumin	967:986	At pH 7.4, the microbeads were dissolved and formed a viscous solution that showed ability to protect bovine serum albumin from glycation induced by both fructose and methylglyoxal.					
30884721	8	41	from	system	1156:1161	arg1	CE/kg					1175:1179	78 mmol CE/kg	1167:1179	78 mmol CE/kg of dry microbeads	1167:1197	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	41	from	system	1156:1161	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	41	from	system	1156:1161	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	42	theme	fructose	1141:1148	arg1	system					1156:1161	the fructose model system	1137:1161	the fructose model system	1137:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	5	43	theme	microbeads	660:669	arg1	compounds					643:651	total phenolic compounds	628:651	total phenolic compounds of the microbeads	628:669	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	10	44	theme	released	1521:1528	arg1	system					1530:1535	a pH controlled released system	1505:1535	a pH controlled released system for GS phenolics	1505:1552	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	1	45	theme	dicarbonyl	188:197	arg1	cause					231:235	the leading cause	219:235	the leading cause of hyperglycaemia damage	219:260	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	45	theme	dicarbonyl	188:197	arg1	sugars					178:183	reducing sugars	169:183	reducing sugars	169:183	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	1	45	theme	dicarbonyl	188:197	arg1	compounds					199:207	dicarbonyl compounds	188:207	dicarbonyl compounds	188:207	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	0	46	theme	phenolics	33:41	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.	0:92	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	8	47	theme	CE	1109:1110	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	47	theme	CE	1109:1110	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	48	theme	dry	1184:1186	arg1	microbeads					1188:1197	dry microbeads	1184:1197	dry microbeads	1184:1197	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	10	49	theme	study	1452:1456	arg1	results					1436:1442	results	1436:1442	results of this study	1436:1456	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	10	50	theme	controlled	1510:1519	arg1	system					1530:1535	a pH controlled released system	1505:1535	a pH controlled released system for GS phenolics	1505:1552	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	0	51	theme	controlled	50:59	arg1	release					61:67	pH controlled release	47:67	pH controlled release of antiglycation agents	47:91	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	1	52	theme	inflammatory	281:292	arg1	diseases					294:301	inflammatory diseases	281:301	inflammatory diseases	281:301	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	7	53	theme	viscous	919:925	arg1	solution					927:934	a viscous solution	917:934	a viscous solution that showed ability to protect bovine serum albumin from glycation induced by both fructose and methylglyoxal	917:1044	At pH 7.4, the microbeads were dissolved and formed a viscous solution that showed ability to protect bovine serum albumin from glycation induced by both fructose and methylglyoxal.					
30884721	1	54	theme	GS	106:107	arg1	phenolics					110:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics	94:118	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	0	55	theme	pH	47:48	arg1	release					61:67	pH controlled release	47:67	pH controlled release of antiglycation agents	47:91	Microencapsulation of grape skin phenolics for pH controlled release of antiglycation agents.					
30884721	11	56	theme	advanced-glycation-endproducts	1621:1650	arg1	diseases					1660:1667	advanced-glycation-endproducts related diseases	1621:1667	advanced-glycation-endproducts related diseases	1621:1667	This functionalized polymer could be applied in the prevention of advanced-glycation-endproducts related diseases.					
30884721	5	57	theme	total	628:632	arg1	compounds					643:651	total phenolic compounds	628:651	total phenolic compounds of the microbeads	628:669	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	6	58	dep	catechin	763:770	arg1	release					788:794	release	788:794	release	788:794	The percent release depended on the compound: procyanidin B1 release was 74%, catechin and epicatechin release was ~ 50%, while anthocyanin and flavonol release was less than 11%.					
30884721	8	59	from	microbeads	1123:1132	arg1	system					1156:1161	the fructose model system	1137:1161	the fructose model system	1137:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	60	theme	78	1167:1168	arg1	mmol					1170:1173	mmol	1170:1173	mmol	1170:1173	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	4	61	theme	Encapsulation	507:519	arg1	efficiency					521:530	Encapsulation efficiency	507:530	Encapsulation efficiency for total phenolics	507:550	Encapsulation efficiency for total phenolics was 68%.					
30884721	5	62	theme	phenolic	634:641	arg1	compounds					643:651	total phenolic compounds	628:651	total phenolic compounds of the microbeads	628:669	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	3	63	theme	vibrating	440:448	arg1	method					457:462	a vibrating nozzle method	438:462	a vibrating nozzle method using calcium chloride as hardening agent	438:504	Microbeads were obtained by a vibrating nozzle method using calcium chloride as hardening agent.					
30884721	5	64	theme	compounds	643:651	arg1	compounds					643:651	total phenolic compounds	628:651	total phenolic compounds of the microbeads	628:669	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	5	64	theme	compounds	643:651	arg1	%					623:623	only 13%	616:623	only 13% of total phenolic compounds of the microbeads	616:669	At pH 1.4, the alginate microbeads remained intact and only 13% of total phenolic compounds of the microbeads was released.					
30884721	11	65	theme	functionalized	1560:1573	arg1	polymer					1575:1581	This functionalized polymer	1555:1581	This functionalized polymer	1555:1581	This functionalized polymer could be applied in the prevention of advanced-glycation-endproducts related diseases.					
30884721	6	66	theme	percent	689:695	arg1	release					697:703	The percent release	685:703	The percent release	685:703	The percent release depended on the compound: procyanidin B1 release was 74%, catechin and epicatechin release was ~ 50%, while anthocyanin and flavonol release was less than 11%.					
30884721	3	67	theme	nozzle	450:455	arg1	method					457:462	a vibrating nozzle method	438:462	a vibrating nozzle method using calcium chloride as hardening agent	438:504	Microbeads were obtained by a vibrating nozzle method using calcium chloride as hardening agent.					
30884721	9	68	theme	alginate	1355:1362	arg1	carrier					1364:1370	the alginate carrier	1351:1370	the alginate carrier	1351:1370	These values corresponded to 68% and 62% of the expected activity, probably due to interaction between phenolics and the alginate carrier.					
30884721	8	69	theme	model	1150:1154	arg1	system					1156:1161	the fructose model system	1137:1161	the fructose model system	1137:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	70	theme	microbeads	1188:1197	arg1	CE/kg					1175:1179	78 mmol CE/kg	1167:1179	78 mmol CE/kg of dry microbeads	1167:1197	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	70	theme	microbeads	1188:1197	arg1	activity					1065:1072	The antiglycation activity	1047:1072	The antiglycation activity	1047:1072	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	70	theme	microbeads	1188:1197	arg1	/kg					1112:1114	246 mmol catechin equivalents (CE)/kg	1078:1114	246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system	1078:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	8	71	theme	dry	1119:1121	arg1	microbeads					1123:1132	dry microbeads	1119:1132	dry microbeads in the fructose model system	1119:1161	The antiglycation activity was 246 mmol catechin equivalents (CE)/kg of dry microbeads in the fructose model system and 78 mmol CE/kg of dry microbeads in the methylglyoxal model system.					
30884721	10	72	theme	activity	1411:1418	arg1	incomplete					1424:1433	incomplete	1424:1433	incomplete	1424:1433	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	10	72	theme	activity	1411:1418	arg1	recovery					1385:1392	the recovery	1381:1392	the recovery of antiglycation activity	1381:1418	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
30884721	2	73	theme	releasing	392:400	arg1	phenolics					364:372	GS phenolics	361:372	GS phenolics	361:372	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	2	73	theme	releasing	392:400	arg1	system					402:407	a pH dependent releasing system	377:407	a pH dependent releasing system	377:407	In this study, alginate hydrogel was used to encapsulate GS phenolics as a pH dependent releasing system.					
30884721	1	74	theme	structural	132:141	arg1	damage					143:148	structural damage	132:148	structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases	132:301	Grape skin (GS) phenolics can prevent structural damage of proteins due to reducing sugars or dicarbonyl compounds, which is the leading cause of hyperglycaemia damage and is involved in inflammatory diseases.					
30884721	10	75	theme	alginate	1486:1493	arg1	efficiency					1472:1481	the efficiency	1468:1481	the efficiency of alginate to act as a pH controlled released system for GS phenolics	1468:1552	Despite the recovery of antiglycation activity was incomplete, results of this study confirmed the efficiency of alginate to act as a pH controlled released system for GS phenolics.					
29392676	3	0	theme	Secondary	306:314	arg1	walls					316:320	Secondary walls	306:320	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.	306:484	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.					
29392676	5	1	theme	cell	724:727	arg1	wall					729:732	cell wall	724:732	cell wall	724:732	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	4	2	theme	different	640:648	arg1	stages					664:669	different developmental stages	640:669	different developmental stages	640:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	5	3	theme	wall	729:732	arg1	structure					695:703	structure	695:703	structure	695:703	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	5	3	theme	wall	729:732	arg1	composition					709:719	composition	709:719	composition	709:719	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	6	4	theme	wall	852:855	arg1	polysaccharides					857:871	cell wall polysaccharides	847:871	cell wall polysaccharides as their alcohol-insoluble residues (AIR)	847:913	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	5	attach	present	994:1000	arg2	monosaccharides					978:992	the neutral and acidic monosaccharides	955:992	the neutral and acidic monosaccharides present in the wall	955:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	5	attach	present	994:1000	arg1	wall					1009:1012	the wall	1005:1012	the wall	1005:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	6	from	wall	1009:1012	arg1	present					994:1000	present	994:1000	present	994:1000	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	3	7	dep	20	462:463	arg1	to					459:460	to	459:460	to	459:460	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.					
29392676	6	8	theme	cell	847:850	arg1	polysaccharides					857:871	cell wall polysaccharides	847:871	cell wall polysaccharides as their alcohol-insoluble residues (AIR)	847:913	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	9	theme	alcohol-insoluble	882:898	arg1	residues					900:907	their alcohol-insoluble residues	876:907	their alcohol-insoluble residues (AIR)	876:913	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	9	theme	alcohol-insoluble	882:898	arg1	AIR					910:912	AIR	910:912	AIR	910:912	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	5	10	theme	various	757:763	arg1	types					765:769	various types	757:769	various types of plant senescence	757:789	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	1	11	theme	Plant	69:73	arg1	walls					80:84	Plant cell walls	69:84	Plant cell walls	69:84	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	6	12	from	present	994:1000	arg1	wall					1009:1012	the wall	1005:1012	the wall	1005:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	1	13	theme	cell	75:78	arg1	walls					80:84	Plant cell walls	69:84	Plant cell walls	69:84	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	4	14	theme	species	605:611	arg1	walls					580:584	the cell walls	571:584	the cell walls of different plant species, different cell types, and different developmental stages	571:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	0	15	theme	Cell	6:9	arg1	Walls					11:15	Plant Cell Walls	0:15	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.	0:67	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	15	theme	Cell	6:9	arg1	Analysis					59:66	Monosaccharide Composition Analysis	32:66	Monosaccharide Composition Analysis	32:66	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	15	theme	Cell	6:9	arg1	Isolation					18:26	Isolation	18:26	Isolation	18:26	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	4	16	theme	cell	624:627	arg1	types					629:633	different cell types	614:633	different cell types	614:633	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	0	17	theme	Plant	0:4	arg1	Walls					11:15	Plant Cell Walls	0:15	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.	0:67	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	17	theme	Plant	0:4	arg1	Analysis					59:66	Monosaccharide Composition Analysis	32:66	Monosaccharide Composition Analysis	32:66	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	17	theme	Plant	0:4	arg1	Isolation					18:26	Isolation	18:26	Isolation	18:26	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	2	18	theme	minerals	296:303	arg1	amounts					273:279	small amounts	267:279	small amounts of protein and minerals	267:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	18	theme	minerals	296:303	arg1	minerals					296:303	minerals	296:303	minerals	296:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	18	theme	minerals	296:303	arg1	protein					284:290	protein	284:290	protein	284:290	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	4	19	theme	different	614:622	arg1	types					629:633	different cell types	614:633	different cell types	614:633	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	4	20	theme	polysaccharides	499:513	arg1	types					490:494	The types	486:494	The types of polysaccharides and their structure and abundance	486:547	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	1	21	dep	plant	128:132	arg1	growth					134:139	growth	134:139	growth	134:139	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	1	21	dep	plant	128:132	arg1	development					145:155	development	145:155	development	145:155	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	5	22	theme	senescence	780:789	arg1	types					765:769	various types	757:769	various types of plant senescence	757:789	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	5	23	theme	plant	774:778	arg1	senescence					780:789	plant senescence	774:789	plant senescence	774:789	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	4	24	theme	structure	525:533	arg1	types					490:494	The types	486:494	The types of polysaccharides and their structure and abundance	486:547	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	2	25	theme	small	267:271	arg1	amounts					273:279	small amounts	267:279	small amounts of protein and minerals	267:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	25	theme	small	267:271	arg1	minerals					296:303	minerals	296:303	minerals	296:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	25	theme	small	267:271	arg1	protein					284:290	protein	284:290	protein	284:290	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	26	theme	protein	284:290	arg1	amounts					273:279	small amounts	267:279	small amounts of protein and minerals	267:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	26	theme	protein	284:290	arg1	minerals					296:303	minerals	296:303	minerals	296:303	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	26	theme	protein	284:290	arg1	protein					284:290	protein	284:290	protein	284:290	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	6	27	theme	polysaccharides	857:871	arg1	isolation					834:842	the isolation	830:842	the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR)	830:913	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	27	theme	polysaccharides	857:871	arg1	procedures					919:928	procedures	919:928	procedures for the determination of the neutral and acidic monosaccharides present in the wall	919:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	6	28	theme	general	811:817	arg1	method					819:824	a general method	809:824	a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall	809:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	2	29	theme	growing	232:238	arg1	cells					246:250	growing plant cells	232:250	growing plant cells	232:250	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	4	30	theme	plant	599:603	arg1	species					605:611	different plant species	589:611	different plant species	589:611	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	5	31	theme	Significant	672:682	arg1	changes					684:690	Significant changes	672:690	Significant changes in structure and composition of cell wall	672:732	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	5	32	from	changes	684:690	arg1	structure					695:703	structure	695:703	structure	695:703	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	5	32	from	changes	684:690	arg1	composition					709:719	composition	709:719	composition	709:719	Significant changes in structure and composition of cell wall have been described in various types of plant senescence.					
29392676	6	33	theme	neutral	959:965	arg1	monosaccharides					978:992	the neutral and acidic monosaccharides	955:992	the neutral and acidic monosaccharides present in the wall	955:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	4	34	theme	different	589:597	arg1	species					605:611	different plant species	589:611	different plant species	589:611	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	0	35	theme	Composition	47:57	arg1	Walls					11:15	Plant Cell Walls	0:15	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.	0:67	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	35	theme	Composition	47:57	arg1	Analysis					59:66	Monosaccharide Composition Analysis	32:66	Monosaccharide Composition Analysis	32:66	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	6	36	theme	monosaccharides	978:992	arg1	determination					938:950	the determination	934:950	the determination of the neutral and acidic monosaccharides present in the wall	934:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	2	37	theme	walls	214:218	arg1	components					188:197	the major components	178:197	the major components of the primary walls surrounding growing plant cells	178:250	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	37	theme	walls	214:218	arg1	Polysaccharides					158:172	Polysaccharides	158:172	Polysaccharides	158:172	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	4	38	theme	stages	664:669	arg1	walls					580:584	the cell walls	571:584	the cell walls of different plant species, different cell types, and different developmental stages	571:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	0	39	theme	Monosaccharide	32:45	arg1	Walls					11:15	Plant Cell Walls	0:15	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.	0:67	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	39	theme	Monosaccharide	32:45	arg1	Analysis					59:66	Monosaccharide Composition Analysis	32:66	Monosaccharide Composition Analysis	32:66	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	1	40	contain	have	86:89	arg2	roles					101:105	important roles	91:105	important roles	91:105	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	1	40	contain	have	86:89	arg1	walls					80:84	Plant cell walls	69:84	Plant cell walls	69:84	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	2	41	theme	primary	206:212	arg1	walls					214:218	the primary walls	202:218	the primary walls surrounding growing plant cells	202:250	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	4	42	theme	developmental	650:662	arg1	stages					664:669	different developmental stages	640:669	different developmental stages	640:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	1	43	theme	important	91:99	arg1	roles					101:105	important roles	91:105	important roles	91:105	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	6	44	theme	acidic	971:976	arg1	monosaccharides					978:992	the neutral and acidic monosaccharides	955:992	the neutral and acidic monosaccharides present in the wall	955:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
29392676	3	45	dep	%	464:464	arg1	w/w					466:468	w/w	466:468	up to 20% w/w of these walls	456:483	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.					
29392676	0	46	dep	Walls	11:15	arg1	Walls					11:15	Plant Cell Walls	0:15	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.	0:67	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	46	dep	Walls	11:15	arg1	Analysis					59:66	Monosaccharide Composition Analysis	32:66	Monosaccharide Composition Analysis	32:66	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	0	46	dep	Walls	11:15	arg1	Isolation					18:26	Isolation	18:26	Isolation	18:26	Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.					
29392676	4	47	theme	types	629:633	arg1	walls					580:584	the cell walls	571:584	the cell walls of different plant species, different cell types, and different developmental stages	571:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	2	48	theme	major	182:186	arg1	components					188:197	the major components	178:197	the major components of the primary walls surrounding growing plant cells	178:250	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	2	48	theme	major	182:186	arg1	Polysaccharides					158:172	Polysaccharides	158:172	Polysaccharides	158:172	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	1	49	theme	plant	128:132	arg1	phases					118:123	all phases	114:123	all phases of plant growth and development	114:155	Plant cell walls have important roles during all phases of plant growth and development.					
29392676	4	50	theme	cell	575:578	arg1	walls					580:584	the cell walls	571:584	the cell walls of different plant species, different cell types, and different developmental stages	571:669	The types of polysaccharides and their structure and abundance often vary greatly in the cell walls of different plant species, different cell types, and different developmental stages.					
29392676	2	51	theme	plant	240:244	arg1	cells					246:250	growing plant cells	232:250	growing plant cells	232:250	Polysaccharides are the major components of the primary walls surrounding growing plant cells, together with small amounts of protein and minerals.					
29392676	3	52	theme	walls	479:483	arg1	%					464:464	up to 20%	456:464	up to 20% w/w of these walls	456:483	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.					
29392676	3	52	theme	walls	479:483	arg1	walls					479:483	these walls	473:483	these walls	473:483	Secondary walls that are deposited when a cell has ceased to grow are also composed predominantly of polysaccharides, although lignin may account for up to 20% w/w of these walls.					
29392676	6	53	theme	present	994:1000	arg1	monosaccharides					978:992	the neutral and acidic monosaccharides	955:992	the neutral and acidic monosaccharides present in the wall	955:1012	Here we describe a general method for the isolation of cell wall polysaccharides as their alcohol-insoluble residues (AIR) and procedures for the determination of the neutral and acidic monosaccharides present in the wall.					
31100397	9	0	contain	have	1488:1491	arg2	effect					1518:1523	a decidedly hypoglycemic effect	1493:1523	a decidedly hypoglycemic effect	1493:1523	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	9	0	contain	have	1488:1491	arg1	HDPs					1483:1486	HDPs	1483:1486	HDPs	1483:1486	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	2	1	theme	antioxidant	343:353	arg1	yields					283:288	The polysaccharide yields	264:288	The polysaccharide yields	264:288	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	1	theme	antioxidant	343:353	arg1	activities					355:364	antioxidant activities	343:364	antioxidant activities	343:364	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	1	2	theme	Hovenia	225:231	arg1	HDPs					257:260	HDPs	257:260	HDPs	257:260	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	1	2	theme	Hovenia	225:231	arg1	polysaccharides					240:254	extract Hovenia dulcis polysaccharides	217:254	extract Hovenia dulcis polysaccharides (HDPs)	217:261	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	5	3	theme	extraction	913:922	arg1	method					924:929	each extraction method	908:929	each extraction method	908:929	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	8	4	theme	chelating	1423:1431	arg1	activity					1433:1440	higher Fe2+ chelating activity	1411:1440	higher Fe2+ chelating activity	1411:1440	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	1	5	theme	dulcis	233:238	arg1	HDPs					257:260	HDPs	257:260	HDPs	257:260	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	1	5	theme	dulcis	233:238	arg1	polysaccharides					240:254	extract Hovenia dulcis polysaccharides	217:254	extract Hovenia dulcis polysaccharides (HDPs)	217:261	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	4	6	theme	%	737:737	arg1	yield					717:721	the maximum HWE-HDP yield	697:721	the maximum HWE-HDP yield of 6.89 ± 0.21%	697:737	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	8	7	theme	superior	1306:1313	arg1	power					1324:1328	superior reducing power	1306:1328	superior reducing power	1306:1328	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	7	8	theme	HDPs	1115:1118	arg1	solutions					1120:1128	the HDPs solutions	1111:1128	the HDPs solutions	1111:1128	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	8	9	dep	displayed	1237:1245	arg1	whereas					1384:1390	whereas	1384:1390	whereas	1384:1390	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	4	10	theme	HWE-HDP	709:715	arg1	yield					717:721	the maximum HWE-HDP yield	697:721	the maximum HWE-HDP yield of 6.89 ± 0.21%	697:737	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	0	11	theme	rheological	96:106	arg1	properties					108:117	rheological properties	96:117	rheological properties	96:117	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	4	12	theme	maximum	701:707	arg1	yield					717:721	the maximum HWE-HDP yield	697:721	the maximum HWE-HDP yield of 6.89 ± 0.21%	697:737	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	8	13	theme	antioxidant	1254:1264	arg1	activities					1266:1275	better antioxidant activities	1247:1275	better antioxidant activities	1247:1275	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	3	14	theme	response	523:530	arg1	method					540:545	a response surface method	521:545	a response surface method (RSM)	521:551	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	3	14	theme	response	523:530	arg1	RSM					548:550	RSM	548:550	RSM	548:550	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	2	15	theme	hypoglycemic	371:382	arg1	effects					384:390	hypoglycemic effects	371:390	hypoglycemic effects	371:390	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	15	theme	hypoglycemic	371:382	arg1	yields					283:288	The polysaccharide yields	264:288	The polysaccharide yields	264:288	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	6	16	theme	typical	999:1005	arg1	behavior					1022:1029	typical shear thinning behavior	999:1029	typical shear thinning behavior	999:1029	Both HDPs exhibited typical shear thinning behavior and non-Newtonian properties.					
31100397	0	17	theme	Japanese	0:7	arg1	polysaccharides					32:46	Japanese grape (Hovenia dulcis) polysaccharides	0:46	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	1	18	theme	accelerated	154:164	arg1	technology					191:200	accelerated solvent extraction (ASE) technology	154:200	accelerated solvent extraction (ASE) technology	154:200	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	0	19	theme	grape	9:13	arg1	polysaccharides					32:46	Japanese grape (Hovenia dulcis) polysaccharides	0:46	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	7	20	theme	gelatinous	1197:1206	arg1	 > G″					1220:1224	G' > G″	1218:1224	G' > G″	1218:1224	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	20	theme	gelatinous	1197:1206	arg1	behavior					1208:1215	gelatinous behavior	1197:1215	gelatinous behavior (G' > G″)	1197:1225	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	9	21	theme	decidedly	1495:1503	arg1	effect					1518:1523	a decidedly hypoglycemic effect	1493:1523	a decidedly hypoglycemic effect	1493:1523	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	2	22	theme	physicochemical	291:305	arg1	yields					283:288	The polysaccharide yields	264:288	The polysaccharide yields	264:288	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	22	theme	physicochemical	291:305	arg1	properties					307:316	physicochemical properties	291:316	physicochemical properties	291:316	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	1	23	theme	solvent	166:172	arg1	ASE					186:188	ASE	186:188	ASE	186:188	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	1	23	theme	solvent	166:172	arg1	extraction					174:183	solvent extraction	166:183	accelerated solvent extraction (ASE) technology	154:200	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	2	24	theme	extracted	437:445	arg1	HWE-HDPs					464:471	HWE-HDPs	464:471	HWE-HDPs	464:471	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	24	theme	extracted	437:445	arg1	polysaccharides					447:461	ASE-HDPs and hot water extracted polysaccharides	414:461	ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs)	414:472	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	7	25	theme	solutions	1120:1128	arg1	modulus					1073:1079	The storage modulus	1061:1079	The storage modulus (G')	1061:1084	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	25	theme	solutions	1120:1128	arg1	G″					1104:1105	G″	1104:1105	G″	1104:1105	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	25	theme	solutions	1120:1128	arg1	modulus					1095:1101	loss modulus	1090:1101	loss modulus (G″)	1090:1106	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	25	theme	solutions	1120:1128	arg1	G					1082:1082	G'	1082:1083	G'	1082:1083	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	3	26	theme	optimized	558:566	arg1	conditions					572:581	optimized ASE conditions	558:581	optimized ASE conditions comprising 2 cycles at 130 °C for 23 min	558:622	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	1	27	theme	extraction	174:183	arg1	technology					191:200	accelerated solvent extraction (ASE) technology	154:200	accelerated solvent extraction (ASE) technology	154:200	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	0	28	theme	Hovenia	16:22	arg1	polysaccharides					32:46	Japanese grape (Hovenia dulcis) polysaccharides	0:46	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	8	29	theme	scavenging	1364:1373	arg1	ability					1375:1381	scavenging ability	1364:1381	scavenging ability	1364:1381	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	5	30	theme	primary	942:948	arg1	structure					950:958	the primary structure	938:958	the primary structure	938:958	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	2	31	theme	hot	427:429	arg1	water					431:435	hot water	427:435	hot water	427:435	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	9	32	theme	in	1447:1448	arg1	experiments					1456:1466	The in vitro experiments	1443:1466	The in vitro experiments	1443:1466	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	8	33	theme	reducing	1315:1322	arg1	power					1324:1328	superior reducing power	1306:1328	superior reducing power	1306:1328	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	6	34	theme	shear	1007:1011	arg1	behavior					1022:1029	typical shear thinning behavior	999:1029	typical shear thinning behavior	999:1029	Both HDPs exhibited typical shear thinning behavior and non-Newtonian properties.					
31100397	2	35	theme	polysaccharide	268:281	arg1	effects					384:390	hypoglycemic effects	371:390	hypoglycemic effects	371:390	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	35	theme	polysaccharide	268:281	arg1	yields					283:288	The polysaccharide yields	264:288	The polysaccharide yields	264:288	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	35	theme	polysaccharide	268:281	arg1	properties					307:316	physicochemical properties	291:316	physicochemical properties	291:316	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	35	theme	polysaccharide	268:281	arg1	properties					331:340	rheological properties	319:340	rheological properties	319:340	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	35	theme	polysaccharide	268:281	arg1	activities					355:364	antioxidant activities	343:364	antioxidant activities	343:364	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	9	36	theme	hypoglycemic	1505:1516	arg1	effect					1518:1523	a decidedly hypoglycemic effect	1493:1523	a decidedly hypoglycemic effect	1493:1523	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	2	37	theme	ASE-HDPs	414:421	arg1	HWE-HDPs					464:471	HWE-HDPs	464:471	HWE-HDPs	464:471	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	37	theme	ASE-HDPs	414:421	arg1	polysaccharides					447:461	ASE-HDPs and hot water extracted polysaccharides	414:461	ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs)	414:472	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	5	38	theme	molecular	767:775	arg1	Mw					785:786	Mw	785:786	Mw	785:786	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	38	theme	molecular	767:775	arg1	weight					777:782	molecular weight	767:782	molecular weight (Mw) distributions	767:801	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	4	39	theme	%	666:666	arg1	higher					685:690	higher	685:690	higher	685:690	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	4	39	theme	%	666:666	arg1	yield					646:650	The maximum ASE-HDPs yield	625:650	The maximum ASE-HDPs yield of 8.62 ± 0.29%	625:666	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	7	40	theme	storage	1065:1071	arg1	modulus					1073:1079	The storage modulus	1061:1079	The storage modulus (G')	1061:1084	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	40	theme	storage	1065:1071	arg1	G					1082:1082	G'	1082:1083	G'	1082:1083	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	5	41	theme	antioxidant	828:838	arg1	activities					840:849	antioxidant activities	828:849	antioxidant activities	828:849	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	4	42	theme	ASE-HDPs	637:644	arg1	higher					685:690	higher	685:690	higher	685:690	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	4	42	theme	ASE-HDPs	637:644	arg1	yield					646:650	The maximum ASE-HDPs yield	625:650	The maximum ASE-HDPs yield of 8.62 ± 0.29%	625:666	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	0	43	theme	dulcis	24:29	arg1	polysaccharides					32:46	Japanese grape (Hovenia dulcis) polysaccharides	0:46	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	5	44	theme	weight	777:782	arg1	distributions					789:801	molecular weight (Mw) distributions	767:801	molecular weight (Mw) distributions	767:801	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	8	45	theme	better	1247:1252	arg1	activities					1266:1275	better antioxidant activities	1247:1275	better antioxidant activities	1247:1275	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	0	46	dep	polysaccharides	32:46	arg1	insight					53:59	New insight	49:59	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	4	47	theme	maximum	629:635	arg1	higher					685:690	higher	685:690	higher	685:690	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	4	47	theme	maximum	629:635	arg1	yield					646:650	The maximum ASE-HDPs yield	625:650	The maximum ASE-HDPs yield of 8.62 ± 0.29%	625:666	The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21%.					
31100397	5	48	theme	hypoglycemic	856:867	arg1	effects					869:875	hypoglycemic effects	856:875	hypoglycemic effects	856:875	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	49	theme	chemical	744:751	arg1	compositions					753:764	The chemical compositions	740:764	The chemical compositions	740:764	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	3	50	theme	ASE	568:570	arg1	conditions					572:581	optimized ASE conditions	558:581	optimized ASE conditions comprising 2 cycles at 130 °C for 23 min	558:622	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	3	51	theme	ASE-HDPs	487:494	arg1	yield					496:500	The maximum ASE-HDPs yield	475:500	The maximum ASE-HDPs yield	475:500	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	2	52	theme	water	431:435	arg1	HWE-HDPs					464:471	HWE-HDPs	464:471	HWE-HDPs	464:471	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	52	theme	water	431:435	arg1	polysaccharides					447:461	ASE-HDPs and hot water extracted polysaccharides	414:461	ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs)	414:472	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	7	53	theme	loss	1090:1093	arg1	G″					1104:1105	G″	1104:1105	G″	1104:1105	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	7	53	theme	loss	1090:1093	arg1	modulus					1095:1101	loss modulus	1090:1101	loss modulus (G″)	1090:1106	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	2	54	theme	rheological	319:329	arg1	yields					283:288	The polysaccharide yields	264:288	The polysaccharide yields	264:288	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	2	54	theme	rheological	319:329	arg1	properties					331:340	rheological properties	319:340	rheological properties	319:340	The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs).					
31100397	8	55	theme	higher	1411:1416	arg1	activity					1433:1440	higher Fe2+ chelating activity	1411:1440	higher Fe2+ chelating activity	1411:1440	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	9	56	dep	in	1447:1448	arg1	vitro					1450:1454	vitro	1450:1454	vitro	1450:1454	The in vitro experiments indicated that HDPs have a decidedly hypoglycemic effect.					
31100397	8	57	dep	DPPH	1280:1283	arg1	ability					1375:1381	scavenging ability	1364:1381	scavenging ability	1364:1381	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	8	58	theme	superoxide	1347:1356	arg1	anion					1358:1362	superoxide anion	1347:1362	superoxide anion	1347:1362	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	3	59	theme	surface	532:538	arg1	method					540:545	a response surface method	521:545	a response surface method (RSM)	521:551	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	3	59	theme	surface	532:538	arg1	RSM					548:550	RSM	548:550	RSM	548:550	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	8	60	theme	Fe2+	1418:1421	arg1	activity					1433:1440	higher Fe2+ chelating activity	1411:1440	higher Fe2+ chelating activity	1411:1440	ASE-HDPs displayed better antioxidant activities in DPPH and ABTS, as well as superior reducing power and hydroxyl and superoxide anion scavenging ability, whereas HWE-HDPs exhibited higher Fe2+ chelating activity.					
31100397	3	61	theme	maximum	479:485	arg1	yield					496:500	The maximum ASE-HDPs yield	475:500	The maximum ASE-HDPs yield	475:500	The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min.					
31100397	6	62	theme	non-Newtonian	1035:1047	arg1	properties					1049:1058	non-Newtonian properties	1035:1058	non-Newtonian properties	1035:1058	Both HDPs exhibited typical shear thinning behavior and non-Newtonian properties.					
31100397	6	63	theme	thinning	1013:1020	arg1	behavior					1022:1029	typical shear thinning behavior	999:1029	typical shear thinning behavior	999:1029	Both HDPs exhibited typical shear thinning behavior and non-Newtonian properties.					
31100397	0	64	theme	New	49:51	arg1	insight					53:59	New insight	49:59	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.	0:137	Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.					
31100397	7	65	theme	oscillation	1160:1170	arg1	frequency					1172:1180	oscillation frequency	1160:1180	oscillation frequency	1160:1180	The storage modulus (G') and loss modulus (G″) of the HDPs solutions were enhanced with increasing oscillation frequency, and displayed gelatinous behavior (G' > G″).					
31100397	5	66	theme	rheological	804:814	arg1	properties					816:825	rheological properties	804:825	rheological properties	804:825	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	67	theme	polysaccharides	880:894	arg1	distributions					789:801	molecular weight (Mw) distributions	767:801	molecular weight (Mw) distributions	767:801	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	67	theme	polysaccharides	880:894	arg1	properties					816:825	rheological properties	804:825	rheological properties	804:825	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	67	theme	polysaccharides	880:894	arg1	activities					840:849	antioxidant activities	828:849	antioxidant activities	828:849	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	67	theme	polysaccharides	880:894	arg1	compositions					753:764	The chemical compositions	740:764	The chemical compositions	740:764	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	5	67	theme	polysaccharides	880:894	arg1	effects					869:875	hypoglycemic effects	856:875	hypoglycemic effects	856:875	The chemical compositions, molecular weight (Mw) distributions, rheological properties, antioxidant activities, and hypoglycemic effects of polysaccharides varied with each extraction method, while the primary structure remained the same.					
31100397	1	68	theme	extract	217:223	arg1	HDPs					257:260	HDPs	257:260	HDPs	257:260	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
31100397	1	68	theme	extract	217:223	arg1	polysaccharides					240:254	extract Hovenia dulcis polysaccharides	217:254	extract Hovenia dulcis polysaccharides (HDPs)	217:261	In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs).					
30135430	5	0	theme	melanoma	728:735	arg1	growth					747:752	melanoma xenograft growth	728:752	melanoma xenograft growth	728:752	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	7	1	theme	growth	1072:1077	arg1	receptor					1086:1093	insulin-like growth factor receptor	1059:1093	insulin-like growth factor receptor	1059:1093	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	8	2	theme	subtle	1144:1149	arg1	changes					1151:1157	subtle changes	1144:1157	subtle changes in glycan structure	1144:1177	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	5	3	theme	xenograft	737:745	arg1	growth					747:752	melanoma xenograft growth	728:752	melanoma xenograft growth	728:752	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	4	4	theme	gene	449:452	arg1	signature					454:462	This gene signature	444:462	This gene signature	444:462	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	7	5	theme	families	1031:1038	arg1	responses					986:994	reduced signaling responses	968:994	reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	968:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	6	6	theme	GCNT2	840:844	arg1	overexpression					822:835	overexpression	822:835	overexpression of GCNT2	822:844	Conversely, overexpression of GCNT2 decreased melanoma xenograft growth, inhibited colony formation, and increased cell death.					
30135430	6	7	theme	colony	893:898	arg1	formation					900:908	colony formation	893:908	colony formation	893:908	Conversely, overexpression of GCNT2 decreased melanoma xenograft growth, inhibited colony formation, and increased cell death.					
30135430	7	8	theme	focused	942:948	arg1	analyses					950:957	More focused analyses	937:957	More focused analyses	937:957	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	3	9	theme	significant	376:386	arg1	changes					404:410	significant transcriptional changes	376:410	significant transcriptional changes in glycosylation-related genes	376:441	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	4	10	theme	I-branching	534:544	arg1	GCNT2					567:571	GCNT2	567:571	GCNT2	567:571	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	4	10	theme	I-branching	534:544	arg1	glycosyltransferase					546:564	I-branching glycosyltransferase	534:564	I-branching glycosyltransferase	534:564	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	8	11	theme	glycan	1162:1167	arg1	structure					1169:1177	glycan structure	1162:1177	glycan structure	1162:1177	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	7	12	theme	insulin-like	1059:1070	arg1	receptor					1086:1093	insulin-like growth factor receptor	1059:1093	insulin-like growth factor receptor	1059:1093	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	1	13	theme	Cancer	72:77	arg1	cells					79:83	Cancer cells	72:83	Cancer cells	72:83	Cancer cells often display altered cell-surface glycans compared to their nontransformed counterparts.					
30135430	3	14	theme	transcriptional	388:402	arg1	changes					404:410	significant transcriptional changes	376:410	significant transcriptional changes in glycosylation-related genes	376:441	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	7	15	theme	factor	1079:1084	arg1	receptor					1086:1093	insulin-like growth factor receptor	1059:1093	insulin-like growth factor receptor	1059:1093	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	8	16	from	changes	1151:1157	arg1	structure					1169:1177	glycan structure	1162:1177	glycan structure	1162:1177	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	8	17	theme	malignancy-associated	1200:1220	arg1	pathways					1222:1229	several malignancy-associated pathways	1192:1229	several malignancy-associated pathways	1192:1229	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	5	18	theme	GCNT2	712:716	arg1	loss					704:707	loss	704:707	loss of GCNT2	704:716	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	6	19	theme	xenograft	865:873	arg1	growth					875:880	melanoma xenograft growth	856:880	melanoma xenograft growth	856:880	Conversely, overexpression of GCNT2 decreased melanoma xenograft growth, inhibited colony formation, and increased cell death.					
30135430	3	20	theme	glycosylation-related	415:435	arg1	genes					437:441	glycosylation-related genes	415:441	glycosylation-related genes	415:441	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	4	21	theme	glycans	619:625	arg1	loss					587:590	a loss	585:590	a loss of cell-surface I-branched glycans	585:625	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	8	22	theme	several	1192:1198	arg1	pathways					1222:1229	several malignancy-associated pathways	1192:1229	several malignancy-associated pathways	1192:1229	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	6	23	theme	melanoma	856:863	arg1	growth					875:880	melanoma xenograft growth	856:880	melanoma xenograft growth	856:880	Conversely, overexpression of GCNT2 decreased melanoma xenograft growth, inhibited colony formation, and increased cell death.					
30135430	1	24	theme	altered	99:105	arg1	glycans					120:126	altered cell-surface glycans	99:126	altered cell-surface glycans	99:126	Cancer cells often display altered cell-surface glycans compared to their nontransformed counterparts.					
30135430	0	25	theme	GCNT2/I-branched	8:23	arg1	glycans					25:31	GCNT2/I-branched glycans	8:31	GCNT2/I-branched glycans	8:31	Loss of GCNT2/I-branched glycans enhances melanoma growth and survival.					
30135430	1	26	theme	cell-surface	107:118	arg1	glycans					120:126	altered cell-surface glycans	99:126	altered cell-surface glycans	99:126	Cancer cells often display altered cell-surface glycans compared to their nontransformed counterparts.					
30135430	0	27	theme	melanoma	42:49	arg1	growth					51:56	melanoma growth	42:56	melanoma growth	42:56	Loss of GCNT2/I-branched glycans enhances melanoma growth and survival.					
30135430	3	28	theme	expression-based	294:309	arg1	analyses					311:318	expression-based analyses	294:318	expression-based analyses across cancer lineages	294:341	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	3	29	from	changes	404:410	arg1	genes					437:441	glycosylation-related genes	415:441	glycosylation-related genes	415:441	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	0	30	theme	glycans	25:31	arg1	Loss					0:3	Loss	0:3	Loss of GCNT2/I-branched glycans	0:31	Loss of GCNT2/I-branched glycans enhances melanoma growth and survival.					
30135430	5	31	theme	colony	764:769	arg1	formation					771:779	colony formation	764:779	colony formation	764:779	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	5	32	theme	cell	795:798	arg1	survival					800:807	cell survival	795:807	cell survival	795:807	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	4	33	theme	I-branched	608:617	arg1	glycans					619:625	cell-surface I-branched glycans	595:625	cell-surface I-branched glycans	595:625	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	7	34	gly	glycoprotein	1018:1029	arg1	glycoprotein					1018:1029	two representative glycoprotein families	999:1038	two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	999:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	2	35	theme	functional	184:193	arg1	contributions					195:207	functional contributions	184:207	functional contributions of glycans to cancer initiation and progression	184:255	However, functional contributions of glycans to cancer initiation and progression remain poorly understood.					
30135430	7	36	theme	representative	1003:1016	arg1	families					1031:1038	two representative glycoprotein families	999:1038	two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	999:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	4	37	theme	cell-surface	595:606	arg1	glycans					619:625	cell-surface I-branched glycans	595:625	cell-surface I-branched glycans	595:625	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	3	38	theme	cancer	327:332	arg1	lineages					334:341	cancer lineages	327:341	cancer lineages	327:341	Here, from expression-based analyses across cancer lineages, we found that melanomas exhibit significant transcriptional changes in glycosylation-related genes.					
30135430	6	39	theme	cell	925:928	arg1	death					930:934	cell death	925:934	cell death	925:934	Conversely, overexpression of GCNT2 decreased melanoma xenograft growth, inhibited colony formation, and increased cell death.					
30135430	5	40	theme	clinical	674:681	arg1	progression					683:693	clinical progression	674:693	clinical progression	674:693	We found that GCNT2 inversely correlated with clinical progression and that loss of GCNT2 increased melanoma xenograft growth, promoted colony formation, and enhanced cell survival.					
30135430	7	41	theme	glycoprotein	1018:1029	arg1	families					1031:1038	two representative glycoprotein families	999:1038	two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	999:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	8	42	theme	melanoma	1241:1248	arg1	signaling					1250:1258	melanoma signaling	1241:1258	melanoma signaling	1241:1258	Overall, these studies reveal how subtle changes in glycan structure can regulate several malignancy-associated pathways and alter melanoma signaling, growth, and survival.					
30135430	4	43	theme	normal	491:496	arg1	melanocytes					498:508	normal melanocytes	491:508	normal melanocytes	491:508	This gene signature revealed that, compared to normal melanocytes, melanomas downregulate I-branching glycosyltransferase, GCNT2, leading to a loss of cell-surface I-branched glycans.					
30135430	7	44	theme	reduced	968:974	arg1	responses					986:994	reduced signaling responses	968:994	reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	968:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	2	45	theme	glycans	212:218	arg1	contributions					195:207	functional contributions	184:207	functional contributions of glycans to cancer initiation and progression	184:255	However, functional contributions of glycans to cancer initiation and progression remain poorly understood.					
30135430	1	46	theme	nontransformed	146:159	arg1	counterparts					161:172	their nontransformed counterparts	140:172	their nontransformed counterparts	140:172	Cancer cells often display altered cell-surface glycans compared to their nontransformed counterparts.					
30135430	7	47	theme	signaling	976:984	arg1	responses					986:994	reduced signaling responses	968:994	reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins	968:1107	More focused analyses revealed reduced signaling responses of two representative glycoprotein families modified by GCNT2, insulin-like growth factor receptor and integrins.					
30135430	2	48	dep	cancer	223:228	arg1	initiation					230:239	initiation	230:239	initiation	230:239	However, functional contributions of glycans to cancer initiation and progression remain poorly understood.					
30987427	9	0	theme	molecular	1439:1447	arg1	weight					1449:1454	similar molecular weight	1431:1454	similar molecular weight	1431:1454	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	6	1	theme	low-molecular-weight	921:940	arg1	heparin					942:948	low-molecular-weight heparin	921:948	low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin	921:1089	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	4	2	theme	single	533:538	arg1	species					556:562	a single dominant animal species	531:562	a single dominant animal species	531:562	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	7	3	attach	derived	1197:1203	arg1	intestine					1216:1224	ovine intestine	1210:1224	ovine intestine	1210:1224	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	7	3	attach	derived	1197:1203	arg2	heparin					1189:1195	low-molecular-weight heparin	1168:1195	low-molecular-weight heparin derived from ovine intestine	1168:1224	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	2	4	from	practice	221:228	arg1	management					321:330	the management	317:330	the management of venous thromboembolism	317:356	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	2	4	from	practice	221:228	arg1	dialysis					276:283	kidney dialysis	269:283	kidney dialysis	269:283	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	2	4	from	practice	221:228	arg1	applications					245:256	applications	245:256	applications	245:256	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	2	4	from	practice	221:228	arg1	cardiopulmonary					286:300	cardiopulmonary	286:300	cardiopulmonary bypass	286:307	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	11	5	theme	heparin	1774:1780	arg1	products					1782:1789	these ovine and porcine heparin products	1750:1789	products	1782:1789	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	10	6	theme	low-molecular-weight	1702:1721	arg1	heparin					1723:1729	porcine enoxaparin and ovine low-molecular-weight heparin	1673:1729	heparin	1723:1729	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	10	7	from	differences	1635:1645	arg1	composition					1555:1565	Some disaccharide compositional, oligosaccharide composition	1506:1565	composition	1555:1565	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	10	8	theme	ovine	1696:1700	arg1	heparin					1723:1729	porcine enoxaparin and ovine low-molecular-weight heparin	1673:1729	heparin	1723:1729	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	11	9	theme	preclinical	1811:1821	arg1	evaluation					1823:1832	their preclinical evaluation	1805:1832	their preclinical evaluation	1805:1832	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	10	10	theme	compositional	1524:1536	arg1	composition					1555:1565	Some disaccharide compositional, oligosaccharide composition	1506:1565	composition	1555:1565	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	1	11	theme	used	158:161	arg1	anticoagulants					172:185	widely used clinical anticoagulants	151:185	widely used clinical anticoagulants	151:185	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	11	theme	used	158:161	arg1	Heparin					90:96	Heparin	90:96	Heparin	90:96	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	11	theme	used	158:161	arg1	heparin					127:133	its low-molecular-weight heparin	102:133	its low-molecular-weight heparin	102:133	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	4	12	theme	worldwide	496:504	arg1	dependence					506:515	The worldwide dependence	492:515	The worldwide dependence on the pig as a single dominant animal species	492:562	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	7	13	theme	ovine	1210:1214	arg1	intestine					1216:1224	ovine intestine	1210:1224	ovine intestine	1210:1224	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	8	14	theme	daughter	1298:1305	arg1	heparins					1328:1335	daughter low-molecular-weight heparins	1298:1335	daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins	1298:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	7	15	theme	compositional	1123:1135	arg1	analyses					1137:1144	top-down, bottom-up, and compositional analyses	1098:1144	analyses	1137:1144	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	4	16	theme	critical	599:606	arg1	drug					608:611	this critical drug	594:611	this critical drug quite fragile	594:625	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	10	17	theme	reducing	1574:1581	arg1	ends					1599:1602	nonreducing ends	1587:1602	nonreducing ends	1587:1602	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	10	18	theme	disaccharide	1511:1522	arg1	composition					1555:1565	Some disaccharide compositional, oligosaccharide composition	1506:1565	composition	1555:1565	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	9	19	theme	anticoagulant	1469:1481	arg1	activities					1483:1492	in vitro anticoagulant activities	1460:1492	in vitro anticoagulant activities	1460:1492	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	4	20	theme	other	654:658	arg1	sources					660:666	other sources	654:666	other sources	654:666	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	4	20	theme	other	654:658	arg1	use					707:709	the expanded use	694:709	the expanded use of bovine tissues	694:727	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	9	21	theme	similar	1431:1437	arg1	weight					1449:1454	similar molecular weight	1431:1454	similar molecular weight	1431:1454	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	4	22	from	dependence	506:515	arg1	pig					524:526	the pig	520:526	the pig	520:526	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	2	23	theme	venous	335:340	arg1	thromboembolism					342:356	venous thromboembolism	335:356	venous thromboembolism	335:356	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	9	24	dep	in	1460:1461	arg1	vitro					1463:1467	vitro	1463:1467	vitro	1463:1467	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	2	25	dep	cardiopulmonary	286:300	arg1	bypass					302:307	bypass	302:307	bypass	302:307	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	11	26	theme	porcine	1766:1772	arg1	products					1782:1789	these ovine and porcine heparin products	1750:1789	products	1782:1789	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	9	27	theme	Ovine	1409:1413	arg1	enoxaparins					1415:1425	Ovine enoxaparins	1409:1425	Ovine enoxaparins	1409:1425	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	10	28	dep	composition	1555:1565	arg1	oligosaccharide					1539:1553	Some disaccharide compositional, oligosaccharide composition	1506:1565	oligosaccharide	1539:1553	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	4	29	theme	tissues	721:727	arg1	use					707:709	the expanded use	694:709	the expanded use of bovine tissues	694:727	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	3	30	theme	bovine	458:463	arg1	intestine					465:473	bovine intestine	458:473	bovine intestine	458:473	Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung.					
30987427	0	31	theme	Heparins	35:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of Low-Molecular-Weight Heparins	0:42	Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin.					
30987427	10	32	theme	nonreducing	1587:1597	arg1	ends					1599:1602	nonreducing ends	1587:1602	nonreducing ends	1587:1602	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	8	33	theme	ovine	1372:1376	arg1	heparins					1385:1392	three unfractionated ovine parent heparins	1351:1392	three unfractionated ovine parent heparins	1351:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	1	34	theme	low-molecular-weight	106:125	arg1	anticoagulants					172:185	widely used clinical anticoagulants	151:185	widely used clinical anticoagulants	151:185	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	34	theme	low-molecular-weight	106:125	arg1	Heparin					90:96	Heparin	90:96	Heparin	90:96	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	34	theme	low-molecular-weight	106:125	arg1	heparin					127:133	its low-molecular-weight heparin	102:133	its low-molecular-weight heparin	102:133	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	8	35	theme	heparins	1328:1335	arg1	compositions					1282:1293	the compositions	1278:1293	the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins	1278:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	0	36	theme	Low-Molecular-Weight	14:33	arg1	Heparins					35:42	Low-Molecular-Weight Heparins	14:42	Low-Molecular-Weight Heparins	14:42	Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin.					
30987427	6	37	theme	chemical	986:993	arg1	β-elimination					995:1007	chemical β-elimination	986:1007	chemical β-elimination	986:1007	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	6	37	theme	chemical	986:993	arg1	process					1012:1018	a process	1010:1018	a process currently used to prepare the low-molecular-weight heparin, enoxaparin	1010:1089	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	11	38	theme	clinical	1849:1856	arg1	assessment					1858:1867	ultimately clinical assessment	1838:1867	ultimately clinical assessment	1838:1867	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	8	39	theme	low-molecular-weight	1307:1326	arg1	heparins					1328:1335	daughter low-molecular-weight heparins	1298:1335	daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins	1298:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	4	40	theme	supply	577:582	arg1	chain					584:588	the supply chain	573:588	the supply chain for this critical drug quite fragile	573:625	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	11	41	theme	ovine	1756:1760	arg1	similarity					1736:1745	The similarity	1732:1745	The similarity of these ovine and porcine heparin products	1732:1789	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	3	42	theme	bovine	479:484	arg1	lung					486:489	bovine lung	479:489	bovine lung	479:489	Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung.					
30987427	6	43	theme	low-molecular-weight	1050:1069	arg1	enoxaparin					1080:1089	enoxaparin	1080:1089	enoxaparin	1080:1089	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	6	43	theme	low-molecular-weight	1050:1069	arg1	heparin					1071:1077	the low-molecular-weight heparin	1046:1077	the low-molecular-weight heparin	1046:1077	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	4	44	theme	animal	549:554	arg1	species					556:562	a single dominant animal species	531:562	a single dominant animal species	531:562	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	7	45	theme	top-down	1098:1105	arg1	bottom-up					1108:1116	top-down, bottom-up, and compositional analyses	1098:1144	bottom-up	1108:1116	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	5	46	theme	porcine	859:865	arg1	tissues					877:883	porcine and ovine tissues	859:883	tissues	877:883	A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues.					
30987427	3	47	theme	porcine	420:426	arg1	intestine					428:436	porcine intestine	420:436	porcine intestine	420:436	Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung.					
30987427	4	48	theme	dominant	540:547	arg1	species					556:562	a single dominant animal species	531:562	a single dominant animal species	531:562	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	9	49	contain	had	1427:1429	arg2	weight					1449:1454	similar molecular weight	1431:1454	similar molecular weight	1431:1454	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	9	49	contain	had	1427:1429	arg1	enoxaparins					1415:1425	Ovine enoxaparins	1409:1425	Ovine enoxaparins	1409:1425	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	9	49	contain	had	1427:1429	arg2	activities					1483:1492	in vitro anticoagulant activities	1460:1492	in vitro anticoagulant activities	1460:1492	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	8	50	theme	unfractionated	1357:1370	arg1	heparins					1385:1392	three unfractionated ovine parent heparins	1351:1392	three unfractionated ovine parent heparins	1351:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	0	51	theme	Ovine	58:62	arg1	Heparins					64:71	Ovine Heparins	58:71	Ovine Heparins With Enoxaparin	58:87	Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin.					
30987427	10	52	theme	intact	1608:1613	arg1	differences					1635:1645	intact chain compositional differences	1608:1645	intact chain compositional differences	1608:1645	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	2	53	theme	medicine	233:240	arg1	practice					221:228	the practice	217:228	the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism	217:356	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	1	54	dep	Heparin	90:96	arg1	derivatives					135:145	derivatives	135:145	derivatives	135:145	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	9	55	theme	in	1460:1461	arg1	activities					1483:1492	in vitro anticoagulant activities	1460:1492	in vitro anticoagulant activities	1460:1492	Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox.					
30987427	10	56	theme	compositional	1621:1633	arg1	differences					1635:1645	intact chain compositional differences	1608:1645	intact chain compositional differences	1608:1645	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	10	57	from	ends	1599:1602	arg1	composition					1555:1565	Some disaccharide compositional, oligosaccharide composition	1506:1565	composition	1555:1565	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	7	58	theme	low-molecular-weight	1168:1187	arg1	heparin					1189:1195	low-molecular-weight heparin	1168:1195	low-molecular-weight heparin derived from ovine intestine	1168:1224	Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin.					
30987427	4	59	theme	bovine	714:719	arg1	tissues					721:727	bovine tissues	714:727	bovine tissues	714:727	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	4	60	theme	fragile	619:625	arg1	drug					608:611	this critical drug	594:611	this critical drug quite fragile	594:625	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	10	61	theme	chain	1615:1619	arg1	differences					1635:1645	intact chain compositional differences	1608:1645	intact chain compositional differences	1608:1645	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	5	62	theme	low-molecular-weight	815:834	arg1	heparins					836:843	low-molecular-weight heparins	815:843	low-molecular-weight heparins	815:843	A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues.					
30987427	0	63	with	Heparins	64:71	arg1	Enoxaparin					78:87	Enoxaparin	78:87	Enoxaparin	78:87	Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin.					
30987427	2	64	theme	thromboembolism	342:356	arg1	management					321:330	the management	317:330	the management of venous thromboembolism	317:356	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	8	65	theme	parent	1378:1383	arg1	heparins					1385:1392	three unfractionated ovine parent heparins	1351:1392	three unfractionated ovine parent heparins	1351:1392	Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared.					
30987427	10	66	theme	porcine	1673:1679	arg1	enoxaparin					1681:1690	porcine enoxaparin and ovine low-molecular-weight heparin	1673:1729	enoxaparin	1681:1690	Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin.					
30987427	1	67	theme	clinical	163:170	arg1	anticoagulants					172:185	widely used clinical anticoagulants	151:185	widely used clinical anticoagulants	151:185	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	67	theme	clinical	163:170	arg1	Heparin					90:96	Heparin	90:96	Heparin	90:96	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	1	67	theme	clinical	163:170	arg1	heparin					127:133	its low-molecular-weight heparin	102:133	its low-molecular-weight heparin	102:133	Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants.					
30987427	5	68	theme	ovine	871:875	arg1	tissues					877:883	porcine and ovine tissues	859:883	tissues	877:883	A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues.					
30987427	11	69	theme	products	1782:1789	arg1	similarity					1736:1745	The similarity	1732:1745	The similarity of these ovine and porcine heparin products	1732:1789	The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.					
30987427	5	70	theme	laboratories	742:753	arg1	number					732:737	A number	730:737	A number of laboratories	730:753	A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues.					
30987427	3	71	attach	derived	386:392	arg2	drugs					376:380	these drugs	370:380	these drugs	370:380	Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung.					
30987427	3	71	attach	derived	386:392	arg1	livestock					399:407	livestock	399:407	livestock	399:407	Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung.					
30987427	2	72	theme	kidney	269:274	arg1	dialysis					276:283	kidney dialysis	269:283	kidney dialysis	269:283	These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.					
30987427	4	73	theme	drugs	677:681	arg1	sources					660:666	other sources	654:666	other sources	654:666	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	4	73	theme	drugs	677:681	arg1	use					707:709	the expanded use	694:709	the expanded use of bovine tissues	694:727	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30987427	6	74	theme	ovine	964:968	arg1	heparin					970:976	ovine heparin	964:976	ovine heparin	964:976	This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin.					
30987427	4	75	theme	expanded	698:705	arg1	use					707:709	the expanded use	694:709	the expanded use of bovine tissues	694:727	The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues.					
30167786	3	0	theme	structural	531:540	arg1	antioxidant					576:586	antioxidant	576:586	antioxidant	576:586	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	0	theme	structural	531:540	arg1	activity					611:618	tyrosinase inhibitory activity	589:618	tyrosinase inhibitory activity	589:618	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	0	theme	structural	531:540	arg1	activies					635:642	antifungal activies	624:642	antifungal activies	624:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	0	theme	structural	531:540	arg1	properties					557:566	their compositional, structural and biological properties	510:566	their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies	510:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	2	1	theme	negligible	417:426	arg1	lipids					451:456	lipids	451:456	lipids	451:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	1	theme	negligible	417:426	arg1	proteins					438:445	proteins	438:445	proteins	438:445	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	1	theme	negligible	417:426	arg1	amount					428:433	negligible amount	417:433	WSPs along with negligible amount of proteins and lipids	401:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	1	2	theme	water-soluble	190:202	arg1	WSPs					221:224	WSPs	221:224	WSPs	221:224	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	2	theme	water-soluble	190:202	arg1	polysaccharides					204:218	water-soluble polysaccharides	190:218	water-soluble polysaccharides (WSPs)	190:225	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	3	3	theme	inhibitory	600:609	arg1	activity					611:618	tyrosinase inhibitory activity	589:618	tyrosinase inhibitory activity	589:618	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	4	theme	biological	546:555	arg1	antioxidant					576:586	antioxidant	576:586	antioxidant	576:586	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	4	theme	biological	546:555	arg1	activity					611:618	tyrosinase inhibitory activity	589:618	tyrosinase inhibitory activity	589:618	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	4	theme	biological	546:555	arg1	activies					635:642	antifungal activies	624:642	antifungal activies	624:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	4	theme	biological	546:555	arg1	properties					557:566	their compositional, structural and biological properties	510:566	their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies	510:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	2	5	theme	Compositional	326:338	arg1	analysis					340:347	Compositional analysis	326:347	Compositional analysis of these BAS	326:360	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	1	6	from	species	317:323	arg1	substances					263:272	the significant bioactive substances	237:272	the significant bioactive substances (BAS) from these photosynthetic microalgae species	237:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	6	from	species	317:323	arg1	BAS					275:277	BAS	275:277	BAS	275:277	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	4	7	theme	antioxidant	679:689	arg1	activities					729:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	These WSPs showed the significant antioxidant, antifungal and tyrosinase inhibitory activities, respectively.					
30167786	1	8	theme	polysaccharides	204:218	arg1	characteristics					171:185	the biological characteristics	156:185	the biological characteristics of water-soluble polysaccharides (WSPs)	156:225	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	5	9	theme	biological	833:842	arg1	moieties					844:851	biological moieties	833:851	biological moieties	833:851	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	4	10	theme	tyrosinase	707:716	arg1	activities					729:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	These WSPs showed the significant antioxidant, antifungal and tyrosinase inhibitory activities, respectively.					
30167786	0	11	theme	biomedical	7:16	arg1	applications					18:29	Potent biomedical applications	0:29	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.	0:101	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	1	12	theme	Microalgae	103:112	arg1	species					126:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	0	13	theme	Potent	0:5	arg1	applications					18:29	Potent biomedical applications	0:29	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.	0:101	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	1	14	theme	photosynthetic	291:304	arg1	species					317:323	these photosynthetic microalgae species	285:323	these photosynthetic microalgae species	285:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	4	15	theme	inhibitory	718:727	arg1	activities					729:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	These WSPs showed the significant antioxidant, antifungal and tyrosinase inhibitory activities, respectively.					
30167786	1	16	theme	Tetraselmis	114:124	arg1	species					126:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	17	theme	microalgae	306:315	arg1	species					317:323	these photosynthetic microalgae species	285:323	these photosynthetic microalgae species	285:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	3	18	theme	tyrosinase	589:598	arg1	activity					611:618	tyrosinase inhibitory activity	589:618	tyrosinase inhibitory activity	589:618	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	5	19	theme	potent	900:905	arg1	activities					918:927	specific potent biological activities	891:927	specific potent biological activities	891:927	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	2	20	theme	lipids	451:456	arg1	lipids					451:456	lipids	451:456	lipids	451:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	20	theme	lipids	451:456	arg1	proteins					438:445	proteins	438:445	proteins	438:445	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	20	theme	lipids	451:456	arg1	amount					428:433	negligible amount	417:433	WSPs along with negligible amount of proteins and lipids	401:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	1	21	used	used	139:142	arg2	species					126:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species	103:132	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	2	22	theme	proteins	438:445	arg1	lipids					451:456	lipids	451:456	lipids	451:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	22	theme	proteins	438:445	arg1	proteins					438:445	proteins	438:445	proteins	438:445	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	2	22	theme	proteins	438:445	arg1	amount					428:433	negligible amount	417:433	WSPs along with negligible amount of proteins and lipids	401:456	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	0	23	theme	polysaccharides	43:57	arg1	applications					18:29	Potent biomedical applications	0:29	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.	0:101	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	5	24	theme	study	776:780	arg1	outcomes					759:766	The outcomes	755:766	The outcomes of this study	755:780	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	0	25	from	species	94:100	arg1	applications					18:29	Potent biomedical applications	0:29	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.	0:101	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	0	26	theme	isolated	34:41	arg1	polysaccharides					43:57	isolated polysaccharides	34:57	isolated polysaccharides	34:57	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	5	27	theme	moieties	844:851	arg1	source					823:828	the potent source	812:828	the potent source of biological moieties for further investigations	812:878	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	5	27	theme	moieties	844:851	arg1	WSPs					800:803	WSPs	800:803	WSPs	800:803	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	4	28	theme	significant	667:677	arg1	activities					729:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	These WSPs showed the significant antioxidant, antifungal and tyrosinase inhibitory activities, respectively.					
30167786	4	29	theme	antifungal	692:701	arg1	activities					729:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	the significant antioxidant, antifungal and tyrosinase inhibitory activities	663:738	These WSPs showed the significant antioxidant, antifungal and tyrosinase inhibitory activities, respectively.					
30167786	0	30	theme	marine	64:69	arg1	species					94:100	marine microalgae Tetraselmis species	64:100	marine microalgae Tetraselmis species	64:100	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	5	31	theme	potent	816:821	arg1	source					823:828	the potent source	812:828	the potent source of biological moieties for further investigations	812:878	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	5	31	theme	potent	816:821	arg1	WSPs					800:803	WSPs	800:803	WSPs	800:803	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	3	32	theme	antifungal	624:633	arg1	activies					635:642	antifungal activies	624:642	antifungal activies	624:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	5	33	theme	biological	907:916	arg1	activities					918:927	specific potent biological activities	891:927	specific potent biological activities	891:927	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	3	34	theme	compositional	516:528	arg1	antioxidant					576:586	antioxidant	576:586	antioxidant	576:586	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	34	theme	compositional	516:528	arg1	activity					611:618	tyrosinase inhibitory activity	589:618	tyrosinase inhibitory activity	589:618	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	34	theme	compositional	516:528	arg1	activies					635:642	antifungal activies	624:642	antifungal activies	624:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	3	34	theme	compositional	516:528	arg1	properties					557:566	their compositional, structural and biological properties	510:566	their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies	510:642	WSPs were partially purified and characterized for their compositional, structural and biological properties such as antioxidant, tyrosinase inhibitory activity and antifungal activies.					
30167786	5	35	theme	further	857:863	arg1	investigations					865:878	further investigations	857:878	further investigations	857:878	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30167786	2	36	dep	along	406:410	arg1	with					412:415	with	412:415	with	412:415	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	1	37	theme	significant	241:251	arg1	substances					263:272	the significant bioactive substances	237:272	the significant bioactive substances (BAS) from these photosynthetic microalgae species	237:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	37	theme	significant	241:251	arg1	BAS					275:277	BAS	275:277	BAS	275:277	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	2	38	theme	BAS	358:360	arg1	analysis					340:347	Compositional analysis	326:347	Compositional analysis of these BAS	326:360	Compositional analysis of these BAS shows that they are mainly composed of WSPs along with negligible amount of proteins and lipids.					
30167786	1	39	theme	bioactive	253:261	arg1	substances					263:272	the significant bioactive substances	237:272	the significant bioactive substances (BAS) from these photosynthetic microalgae species	237:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	39	theme	bioactive	253:261	arg1	BAS					275:277	BAS	275:277	BAS	275:277	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	0	40	theme	Tetraselmis	82:92	arg1	species					94:100	marine microalgae Tetraselmis species	64:100	marine microalgae Tetraselmis species	64:100	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	1	41	theme	biological	160:169	arg1	characteristics					171:185	the biological characteristics	156:185	the biological characteristics of water-soluble polysaccharides (WSPs)	156:225	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	42	theme	substances	263:272	arg1	substances					263:272	the significant bioactive substances	237:272	the significant bioactive substances (BAS) from these photosynthetic microalgae species	237:323	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	42	theme	substances	263:272	arg1	one					230:232	one	230:232	one	230:232	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	1	42	theme	substances	263:272	arg1	BAS					275:277	BAS	275:277	BAS	275:277	Microalgae Tetraselmis species were used to evaluate the biological characteristics of water-soluble polysaccharides (WSPs) as one of the significant bioactive substances (BAS) from these photosynthetic microalgae species.					
30167786	0	43	theme	microalgae	71:80	arg1	species					94:100	marine microalgae Tetraselmis species	64:100	marine microalgae Tetraselmis species	64:100	Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species.					
30167786	5	44	theme	specific	891:898	arg1	activities					918:927	specific potent biological activities	891:927	specific potent biological activities	891:927	The outcomes of this study demonstrated that WSPs can be the potent source of biological moieties for further investigations along with specific potent biological activities.					
30223004	2	0	from	pyrophosphorylase	444:460	arg1	E. gracilis					483:493	E. gracilis	483:493	E. gracilis	483:493	Herein, we report the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis.					
30223004	3	1	theme	gene	533:536	arg1	expression					515:524	heterologous expression	502:524	heterologous expression of the gene in Escherichia coli	502:556	After heterologous expression of the gene in Escherichia coli, the recombinant enzyme was characterized structural and functionally.					
30223004	9	2	theme	cytosolic	1444:1452	arg1	localization					1480:1491	a cytosolic (mainly in the flagellum) localization	1442:1491	a cytosolic (mainly in the flagellum) localization of the enzyme	1442:1505	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	0	3	theme	Kinetic	77:83	arg1	characterization					85:100	Kinetic characterization	77:100	Kinetic characterization	77:100	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	0	4	from	paramylon	12:20	arg1	gracilis					67:74	Euglena gracilis	59:74	Euglena gracilis	59:74	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	3	5	theme	recombinant	563:573	arg1	enzyme					575:580	the recombinant enzyme	559:580	the recombinant enzyme	559:580	After heterologous expression of the gene in Escherichia coli, the recombinant enzyme was characterized structural and functionally.					
30223004	4	6	theme	purified	636:643	arg1	EgrUDP-GlcPPase					645:659	Highly purified EgrUDP-GlcPPase	629:659	Highly purified EgrUDP-GlcPPase	629:659	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	6	7	with	reduction	989:997	arg1	thioredoxin					1022:1032	thioredoxin	1022:1032	thioredoxin	1022:1032	Oxidation by hydrogen peroxide inactivated the enzyme, an effect reversed by reduction with dithiothreitol or thioredoxin.					
30223004	6	7	with	reduction	989:997	arg1	dithiothreitol					1004:1017	dithiothreitol	1004:1017	dithiothreitol	1004:1017	Oxidation by hydrogen peroxide inactivated the enzyme, an effect reversed by reduction with dithiothreitol or thioredoxin.					
30223004	0	8	theme	cellular	117:124	arg1	localization					126:137	cellular localization	117:137	cellular localization	117:137	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	6	9	theme	hydrogen	925:932	arg1	peroxide					934:941	hydrogen peroxide	925:941	hydrogen peroxide	925:941	Oxidation by hydrogen peroxide inactivated the enzyme, an effect reversed by reduction with dithiothreitol or thioredoxin.					
30223004	7	10	theme	residues	1124:1131	arg1	pair					1101:1104	no pair	1098:1104	no pair of the 7 cysteine residues	1098:1131	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	0	11	from	metabolism	45:54	arg1	gracilis					67:74	Euglena gracilis	59:74	Euglena gracilis	59:74	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	5	12	dep	used	839:842	arg1	efficiency					866:875	lower catalytic efficiency	850:875	lower catalytic efficiency	850:875	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	8	13	theme	inactive	1309:1316	arg1	conformations					1329:1341	many enzymatically inactive structural conformations	1290:1341	many enzymatically inactive structural conformations; which were also detected in vivo	1290:1375	Electrophoresis studies suggest that, after oxidation, the enzyme arranges in many enzymatically inactive structural conformations; which were also detected in vivo.					
30223004	2	14	theme	gene	403:406	arg1	cloning					390:396	the molecular cloning	376:396	the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis	376:493	Herein, we report the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis.					
30223004	9	15	from	localization	1480:1491	arg1	flagellum					1469:1477	the flagellum	1465:1477	the flagellum	1465:1477	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	3	16	from	expression	515:524	arg1	coli					553:556	Escherichia coli	541:556	Escherichia coli	541:556	After heterologous expression of the gene in Escherichia coli, the recombinant enzyme was characterized structural and functionally.					
30223004	1	17	dep	oligo	178:182	arg1	including					205:213	including	205:213	including paramylon	205:223	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	4	18	theme	UDP-glucose	724:734	arg1	synthesis					711:719	synthesis	711:719	synthesis of UDP-glucose with a Vmax of 3350 U.mg-1	711:761	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	7	19	theme	cysteine	1115:1122	arg1	residues					1124:1131	the 7 cysteine residues	1109:1131	the 7 cysteine residues	1109:1131	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	0	20	theme	other	26:30	arg1	metabolism					45:54	other carbohydrate metabolism	26:54	other carbohydrate metabolism	26:54	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	1	21	theme	glycosyl	302:309	arg1	transferases					311:322	glycosyl transferases	302:322	glycosyl transferases using UDP-glucose as a substrate	302:355	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	5	22	used	used	839:842	arg2	enzyme					827:832	the enzyme	823:832	the enzyme	823:832	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	8	23	theme	Electrophoresis	1212:1226	arg1	studies					1228:1234	Electrophoresis studies	1212:1234	Electrophoresis studies	1212:1234	Electrophoresis studies suggest that, after oxidation, the enzyme arranges in many enzymatically inactive structural conformations; which were also detected in vivo.					
30223004	2	24	theme	UDP-glucose	432:442	arg1	EgrUDP-GlcPPase					463:477	EgrUDP-GlcPPase	463:477	EgrUDP-GlcPPase	463:477	Herein, we report the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis.					
30223004	2	24	theme	UDP-glucose	432:442	arg1	pyrophosphorylase					444:460	a UDP-glucose pyrophosphorylase	430:460	a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis	430:493	Herein, we report the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis.					
30223004	5	25	theme	catalytic	856:864	arg1	efficiency					866:875	lower catalytic efficiency	850:875	lower catalytic efficiency	850:875	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	5	26	theme	lower	850:854	arg1	efficiency					866:875	lower catalytic efficiency	850:875	lower catalytic efficiency	850:875	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	0	27	theme	pyrophosphorylase	154:170	arg1	structure					103:111	structure	103:111	structure	103:111	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	0	27	theme	pyrophosphorylase	154:170	arg1	localization					126:137	cellular localization	117:137	cellular localization	117:137	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	0	27	theme	pyrophosphorylase	154:170	arg1	characterization					85:100	Kinetic characterization	77:100	Kinetic characterization	77:100	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	9	28	theme	enzyme	1500:1505	arg1	localization					1480:1491	a cytosolic (mainly in the flagellum) localization	1442:1491	a cytosolic (mainly in the flagellum) localization of the enzyme	1442:1505	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	8	29	theme	many	1290:1293	arg1	conformations					1329:1341	many enzymatically inactive structural conformations	1290:1341	many enzymatically inactive structural conformations; which were also detected in vivo	1290:1375	Electrophoresis studies suggest that, after oxidation, the enzyme arranges in many enzymatically inactive structural conformations; which were also detected in vivo.					
30223004	0	30	theme	carbohydrate	32:43	arg1	metabolism					45:54	other carbohydrate metabolism	26:54	other carbohydrate metabolism	26:54	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	7	31	from	close	1136:1140	arg1	structure					1159:1167	the 3D structure	1152:1167	the 3D structure of the protein to form a disulfide bridge	1152:1209	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	0	32	theme	UDP-glucose	142:152	arg1	pyrophosphorylase					154:170	UDP-glucose pyrophosphorylase	142:170	UDP-glucose pyrophosphorylase	142:170	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	4	33	with	synthesis	711:719	arg1	Vmax					743:746	a Vmax	741:746	a Vmax of 3350 U.mg-1	741:761	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	4	34	theme	monomeric	673:681	arg1	structure					683:691	a monomeric structure	671:691	a monomeric structure	671:691	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	1	35	from	critical	230:237	arg1	life-cycle					263:272	the Euglena gracilis life-cycle	242:272	the Euglena gracilis life-cycle	242:272	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	4	36	theme	able	694:697	arg1	structure					683:691	a monomeric structure	671:691	a monomeric structure	671:691	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	0	37	theme	Euglena	59:65	arg1	gracilis					67:74	Euglena gracilis	59:74	Euglena gracilis	59:74	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	9	38	theme	confocal	1387:1394	arg1	microscopy					1409:1418	confocal fluorescence microscopy	1387:1418	confocal fluorescence microscopy	1387:1418	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	7	39	theme	sulfenic	1067:1074	arg1	formation					1081:1089	sulfenic acid formation	1067:1089	sulfenic acid formation	1067:1089	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	4	40	theme	U.mg-1	756:761	arg1	Vmax					743:746	a Vmax	741:746	a Vmax of 3350 U.mg-1	741:761	Highly purified EgrUDP-GlcPPase exhibited a monomeric structure, able to catalyze synthesis of UDP-glucose with a Vmax of 3350 U.mg-1.					
30223004	9	41	theme	fluorescence	1396:1407	arg1	microscopy					1409:1418	confocal fluorescence microscopy	1387:1418	confocal fluorescence microscopy	1387:1418	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	1	42	from	life-cycle	263:272	arg1	critical					230:237	critical	230:237	critical	230:237	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	9	43	dep	flagellum	1469:1477	arg1	mainly					1455:1460	mainly	1455:1460	mainly	1455:1460	Finally, confocal fluorescence microscopy provided evidence for a cytosolic (mainly in the flagellum) localization of the enzyme.					
30223004	7	44	theme	protein	1176:1182	arg1	structure					1159:1167	the 3D structure	1152:1167	the 3D structure of the protein to form a disulfide bridge	1152:1209	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	7	45	theme	acid	1076:1079	arg1	formation					1081:1089	sulfenic acid formation	1067:1089	sulfenic acid formation	1067:1089	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	7	46	from	structure	1159:1167	arg1	close					1136:1140	close	1136:1140	close	1136:1140	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	3	47	theme	heterologous	502:513	arg1	expression					515:524	heterologous expression	502:524	heterologous expression of the gene in Escherichia coli	502:556	After heterologous expression of the gene in Escherichia coli, the recombinant enzyme was characterized structural and functionally.					
30223004	8	48	theme	structural	1318:1327	arg1	conformations					1329:1341	many enzymatically inactive structural conformations	1290:1341	many enzymatically inactive structural conformations; which were also detected in vivo	1290:1375	Electrophoresis studies suggest that, after oxidation, the enzyme arranges in many enzymatically inactive structural conformations; which were also detected in vivo.					
30223004	1	49	theme	Euglena	246:252	arg1	gracilis					254:261	Euglena gracilis	246:261	the Euglena gracilis life-cycle	242:272	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	5	50	theme	preferred	792:800	arg1	substrates					802:811	the preferred substrates	788:811	the preferred substrates	788:811	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	5	50	theme	preferred	792:800	arg1	UTP					779:781	UTP	779:781	UTP	779:781	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	5	50	theme	preferred	792:800	arg1	Glucose-1P					764:773	Glucose-1P	764:773	Glucose-1P	764:773	Glucose-1P and UTP were the preferred substrates, although the enzyme also used (with lower catalytic efficiency) TTP, galactose-1P and mannose-1P.					
30223004	7	51	theme	redox	1039:1043	arg1	process					1045:1051	The redox process	1035:1051	The redox process	1035:1051	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	1	52	theme	gracilis	254:261	arg1	life-cycle					263:272	the Euglena gracilis life-cycle	242:272	the Euglena gracilis life-cycle	242:272	Many oligo and polysaccharides (including paramylon) are critical in the Euglena gracilis life-cycle and they are synthesized by glycosyl transferases using UDP-glucose as a substrate.					
30223004	7	53	theme	3D	1156:1157	arg1	structure					1159:1167	the 3D structure	1152:1167	the 3D structure of the protein to form a disulfide bridge	1152:1209	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	7	54	theme	disulfide	1194:1202	arg1	bridge					1204:1209	a disulfide bridge	1192:1209	a disulfide bridge	1192:1209	The redox process would involve sulfenic acid formation, since no pair of the 7 cysteine residues is close enough in the 3D structure of the protein to form a disulfide bridge.					
30223004	0	55	dep	Elucidating	0:10	arg1	structure					103:111	structure	103:111	structure	103:111	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	0	55	dep	Elucidating	0:10	arg1	localization					126:137	cellular localization	117:137	cellular localization	117:137	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	0	55	dep	Elucidating	0:10	arg1	characterization					85:100	Kinetic characterization	77:100	Kinetic characterization	77:100	Elucidating paramylon and other carbohydrate metabolism in Euglena gracilis: Kinetic characterization, structure and cellular localization of UDP-glucose pyrophosphorylase.					
30223004	2	56	theme	molecular	380:388	arg1	cloning					390:396	the molecular cloning	376:396	the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis	376:493	Herein, we report the molecular cloning of a gene putatively coding for a UDP-glucose pyrophosphorylase (EgrUDP-GlcPPase) in E. gracilis.					
31896526	8	0	theme	class-I	1651:1657	arg1	context					1607:1613	the context	1603:1613	the context of major histocompatibility complex class-I (MHC-I)	1603:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	6	1	theme	CD8+	1201:1204	arg1	cells					1208:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	4	2	theme	heparan	605:611	arg1	HS					622:623	HS	622:623	HS	622:623	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	4	2	theme	heparan	605:611	arg1	sulfate					613:619	heparan sulfate	605:619	a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1	603:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	1	3	theme	recent	97:102	arg1	points					113:118	recent research points	97:118	recent research points to the importance of glycans in cancer immunity	97:166	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	8	4	from	presentation	1587:1598	arg1	context					1607:1613	the context	1603:1613	the context of major histocompatibility complex class-I (MHC-I)	1603:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	7	5	theme	species	1506:1512	arg1	sulfation					1465:1473	reduced sulfation	1457:1473	reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS	1457:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	6	6	theme	TIL	1196:1198	arg1	cells					1208:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	2	7	theme	robust	393:398	arg1	responses					400:408	robust responses	393:408	robust responses	393:408	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	6	8	dep	cells	1167:1171	arg1	purified					1214:1221	purified	1214:1221	purified ex-vivo	1214:1229	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	6	8	dep	cells	1167:1171	arg1	tested					1236:1241	tested	1236:1241	tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals	1236:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	6	9	theme	cytolytic	1272:1280	arg1	assays					1282:1287	pooled effector-to-target cytolytic assays	1246:1287	pooled effector-to-target cytolytic assays	1246:1287	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	8	10	theme	under-sulfation	1743:1757	arg1	setting					1729:1735	the setting	1725:1735	the setting of HS under-sulfation	1725:1757	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	7	11	theme	tri-sulfated	1480:1491	arg1	species					1506:1512	a tri-sulfated disaccharide species	1478:1512	a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS	1478:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	7	11	theme	tri-sulfated	1480:1491	arg1	abundant					1536:1543	abundant	1536:1543	abundant	1536:1543	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	6	12	theme	tumor-infiltrating	1176:1193	arg1	cells					1208:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	6	13	theme	pooled	1246:1251	arg1	assays					1282:1287	pooled effector-to-target cytolytic assays	1246:1287	pooled effector-to-target cytolytic assays	1246:1287	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	2	14	theme	recent	304:309	arg1	therapies					311:319	some recent therapies	299:319	some recent therapies	299:319	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	5	15	theme	major	884:888	arg1	proteoglycan					896:907	a major DC HS proteoglycan	882:907	a major DC HS proteoglycan (syndecan-4)	882:920	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	15	theme	major	884:888	arg1	syndecan-4					910:919	syndecan-4	910:919	syndecan-4	910:919	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	7	16	theme	reduced	1420:1426	arg1	sulfation					1436:1444	reduced overall sulfation	1420:1444	reduced overall sulfation	1420:1444	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	5	17	theme	T	936:936	arg1	cells					938:942	splenic CD8+ T cells	923:942	splenic CD8+ T cells	923:942	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	8	18	theme	immunologic	1940:1950	arg1	targeting					1952:1960	immunologic targeting	1940:1960	immunologic targeting of HS proteoglycans in cancer	1940:1990	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	19	from	mutation	643:650	arg1	Ndst1					679:683	the HS sulfating enzyme Ndst1	655:683	the HS sulfating enzyme Ndst1	655:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	8	20	theme	regulatory	1781:1790	arg1	role					1792:1795	a likely regulatory role	1772:1795	a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface;	1772:1856	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	21	theme	Ndst1f/f	720:727	arg1	DCs					715:717	predominantly conventional DCs	688:717	predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation)	688:747	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	4	21	theme	Ndst1f/f	720:727	arg1	mutation					739:746	Ndst1f/f CD11cCre+ mutation	720:746	Ndst1f/f CD11cCre+ mutation	720:746	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	8	22	theme	proteoglycans	1968:1980	arg1	targeting					1952:1960	immunologic targeting	1940:1960	immunologic targeting of HS proteoglycans in cancer	1940:1990	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	2	23	theme	lung	219:222	arg1	carcinoma					224:232	lung carcinoma	219:232	lung carcinoma among other tumors	219:251	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	8	24	theme	antigen-specific	1903:1918	arg1	responses					1927:1935	antigen-specific T cell responses	1903:1935	antigen-specific T cell responses	1903:1935	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	8	25	from	effects	1714:1720	arg1	setting					1729:1735	the setting	1725:1735	the setting of HS under-sulfation	1725:1757	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	6	26	theme	respective	1314:1323	arg1	animals					1325:1331	respective animals	1314:1331	respective animals	1314:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	2	27	theme	anti-tumor	254:263	arg1	immunity					265:272	anti-tumor immunity	254:272	anti-tumor immunity	254:272	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	5	28	theme	splenic	923:929	arg1	cells					938:942	splenic CD8+ T cells	923:942	splenic CD8+ T cells	923:942	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	6	29	theme	Ndst1f/f	1032:1039	arg1	mutants					1052:1058	Ndst1f/f CD11cCre + mutants	1032:1058	Ndst1f/f CD11cCre + mutants	1032:1058	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	0	30	theme	Proteoglycan	67:78	arg1	Targeting					80:88	Genetic Proteoglycan Targeting	59:88	Genetic Proteoglycan Targeting	59:88	Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting.					
31896526	5	31	from	deficient	869:877	arg1	proteoglycan					896:907	a major DC HS proteoglycan	882:907	a major DC HS proteoglycan (syndecan-4)	882:920	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	31	from	deficient	869:877	arg1	syndecan-4					910:919	syndecan-4	910:919	syndecan-4	910:919	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	32	theme	HS	893:894	arg1	proteoglycan					896:907	a major DC HS proteoglycan	882:907	a major DC HS proteoglycan (syndecan-4)	882:920	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	32	theme	HS	893:894	arg1	syndecan-4					910:919	syndecan-4	910:919	syndecan-4	910:919	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	8	33	theme	mutant	1683:1688	arg1	DCs					1690:1692	mutant DCs	1683:1692	mutant DCs	1683:1692	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	5	34	from	proteoglycan	896:907	arg1	deficient					869:877	deficient	869:877	deficient	869:877	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	35	theme	anti-tumor	961:970	arg1	responses					982:990	increased anti-tumor cytotoxic responses	951:990	increased anti-tumor cytotoxic responses relative to controls	951:1011	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	1	36	theme	glycans	141:147	arg1	importance					127:136	the importance	123:136	the importance of glycans in cancer immunity	123:166	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	4	37	theme	lung	787:790	arg1	carcinoma					792:800	Lewis lung carcinoma	781:800	Lewis lung carcinoma growth	781:807	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	3	38	theme	antigen-specific	441:456	arg1	responses					465:473	tumor antigen-specific immune responses	435:473	tumor antigen-specific immune responses	435:473	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	0	39	theme	Functional	0:9	arg1	Mechanisms					31:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting.					
31896526	8	40	theme	future	1871:1876	arg1	opportunities					1878:1890	future opportunities	1871:1890	future opportunities	1871:1890	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	1	41	theme	research	104:111	arg1	points					113:118	recent research points	97:118	recent research points to the importance of glycans in cancer immunity	97:166	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	7	42	theme	compositional	1344:1356	arg1	analysis					1358:1365	glycan compositional analysis	1337:1365	glycan compositional analysis	1337:1365	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	4	43	theme	marked	760:765	arg1	inhibition					767:776	marked inhibition	760:776	marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells	760:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	0	44	theme	Anti-Tumor	20:29	arg1	Mechanisms					31:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting.					
31896526	4	45	theme	tumor-associated	830:845	arg1	cells					854:858	increased tumor-associated CD8+ T cells	820:858	increased tumor-associated CD8+ T cells	820:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	3	46	theme	endogenous	478:487	arg1	DCs					506:508	DCs	506:508	DCs	506:508	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	3	46	theme	endogenous	478:487	arg1	cells					499:503	endogenous dendritic cells	478:503	endogenous dendritic cells (DCs)	478:509	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	4	47	theme	sulfating	662:670	arg1	Ndst1					679:683	the HS sulfating enzyme Ndst1	655:683	the HS sulfating enzyme Ndst1	655:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	6	48	from	animals	1325:1331	arg1	cells					1303:1307	tumor cells	1297:1307	tumor cells from respective animals	1297:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	4	49	theme	T	852:852	arg1	cells					854:858	increased tumor-associated CD8+ T cells	820:858	increased tumor-associated CD8+ T cells	820:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	7	50	theme	DC	1557:1558	arg1	HS					1560:1561	wildtype DC HS	1548:1561	wildtype DC HS	1548:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	8	51	theme	T	1920:1920	arg1	responses					1927:1935	antigen-specific T cell responses	1903:1935	antigen-specific T cell responses	1903:1935	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	6	52	theme	splenic	1152:1158	arg1	cells					1167:1171	splenic CD8+ T cells	1152:1171	splenic CD8+ T cells	1152:1171	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	8	53	theme	histocompatibility	1624:1641	arg1	complex					1643:1649	major histocompatibility complex	1618:1649	major histocompatibility complex class-I (MHC-I)	1618:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	54	theme	growth	802:807	arg1	inhibition					767:776	marked inhibition	760:776	marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells	760:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	1	55	from	knowledge	169:177	arg1	mechanisms					193:202	functional mechanisms	182:202	functional mechanisms	182:202	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	1	56	theme	cancer	152:157	arg1	immunity					159:166	cancer immunity	152:166	cancer immunity	152:166	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	4	57	theme	sulfate	613:619	arg1	mutation					643:650	a heparan sulfate (HS) loss-of-function mutation	603:650	a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1	603:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	6	58	from	increase	1106:1113	arg1	cytolysis					1129:1137	anti-tumor cytolysis	1118:1137	anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals	1118:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	3	59	theme	specificity	531:541	arg1	standpoint					543:552	a specificity standpoint	529:552	a specificity standpoint	529:552	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	6	60	theme	anti-tumor	1118:1127	arg1	cytolysis					1129:1137	anti-tumor cytolysis	1118:1137	anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals	1118:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	8	61	theme	striking	1705:1712	arg1	effects					1714:1720	more striking effects	1700:1720	more striking effects in the setting of HS under-sulfation	1700:1757	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	6	62	theme	tumor	1297:1301	arg1	cells					1303:1307	tumor cells	1297:1307	tumor cells from respective animals	1297:1331	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	7	63	contain	had	1416:1418	arg1	HS					1368:1369	HS	1368:1369	HS purified from Ndst1f/f CD11cCre + mutant DCs	1368:1414	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	7	63	contain	had	1416:1418	arg2	sulfation					1436:1444	reduced overall sulfation	1420:1444	reduced overall sulfation	1420:1444	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	7	64	theme	disaccharide	1493:1504	arg1	species					1506:1512	a tri-sulfated disaccharide species	1478:1512	a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS	1478:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	7	64	theme	disaccharide	1493:1504	arg1	abundant					1536:1543	abundant	1536:1543	abundant	1536:1543	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	8	65	theme	cell	1922:1925	arg1	responses					1927:1935	antigen-specific T cell responses	1903:1935	antigen-specific T cell responses	1903:1935	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	5	66	theme	deficient	869:877	arg1	mice					864:867	mice	864:867	mice deficient in a major DC HS proteoglycan (syndecan-4)	864:920	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	7	67	theme	reduced	1457:1463	arg1	sulfation					1465:1473	reduced sulfation	1457:1473	reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS	1457:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	4	68	theme	HS	659:660	arg1	Ndst1					679:683	the HS sulfating enzyme Ndst1	655:683	the HS sulfating enzyme Ndst1	655:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	6	69	theme	effector-to-target	1253:1270	arg1	assays					1282:1287	pooled effector-to-target cytolytic assays	1246:1287	pooled effector-to-target cytolytic assays	1246:1287	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	8	70	theme	HS	1740:1741	arg1	under-sulfation					1743:1757	HS under-sulfation	1740:1757	HS under-sulfation	1740:1757	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	1	71	theme	functional	182:191	arg1	mechanisms					193:202	functional mechanisms	182:202	functional mechanisms	182:202	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	4	72	theme	enzyme	672:677	arg1	Ndst1					679:683	the HS sulfating enzyme Ndst1	655:683	the HS sulfating enzyme Ndst1	655:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	6	73	theme	T	1165:1165	arg1	cells					1167:1171	splenic CD8+ T cells	1152:1171	splenic CD8+ T cells	1152:1171	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	4	74	theme	loss-of-function	626:641	arg1	mutation					643:650	a heparan sulfate (HS) loss-of-function mutation	603:650	a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1	603:683	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	8	75	theme	HS	1965:1966	arg1	proteoglycans					1968:1980	HS proteoglycans	1965:1980	HS proteoglycans	1965:1980	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	2	76	theme	other	240:244	arg1	tumors					246:251	other tumors	240:251	other tumors	240:251	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	8	77	from	interface	1847:1855	arg1	role					1792:1795	a likely regulatory role	1772:1795	a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface;	1772:1856	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	8	77	from	interface	1847:1855	arg1	opportunities					1878:1890	future opportunities	1871:1890	future opportunities	1871:1890	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	8	78	theme	likely	1774:1779	arg1	role					1792:1795	a likely regulatory role	1772:1795	a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface;	1772:1856	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	7	79	theme	mutant	1405:1410	arg1	DCs					1412:1414	mutant DCs	1405:1414	mutant DCs	1405:1414	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	6	80	theme	CD11cCre +	1041:1050	arg1	mutants					1052:1058	Ndst1f/f CD11cCre + mutants	1032:1058	Ndst1f/f CD11cCre + mutants	1032:1058	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	4	81	theme	CD11cCre+	729:737	arg1	DCs					715:717	predominantly conventional DCs	688:717	predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation)	688:747	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	4	81	theme	CD11cCre+	729:737	arg1	mutation					739:746	Ndst1f/f CD11cCre+ mutation	720:746	Ndst1f/f CD11cCre+ mutation	720:746	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	8	82	theme	sulfated	1800:1807	arg1	glycans					1809:1815	sulfated glycans	1800:1815	sulfated glycans	1800:1815	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	5	83	theme	DC	890:891	arg1	proteoglycan					896:907	a major DC HS proteoglycan	882:907	a major DC HS proteoglycan (syndecan-4)	882:920	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	5	83	theme	DC	890:891	arg1	syndecan-4					910:919	syndecan-4	910:919	syndecan-4	910:919	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	6	84	theme	T	1206:1206	arg1	cells					1208:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	tumor-infiltrating (TIL) CD8+ T cells	1176:1212	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	4	85	theme	conventional	702:713	arg1	DCs					715:717	predominantly conventional DCs	688:717	predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation)	688:747	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	4	85	theme	conventional	702:713	arg1	mutation					739:746	Ndst1f/f CD11cCre+ mutation	720:746	Ndst1f/f CD11cCre+ mutation	720:746	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	8	86	theme	antigen/MHC-I	1824:1836	arg1	interface					1847:1855	the antigen/MHC-I - T-cell interface	1820:1855	the antigen/MHC-I - T-cell interface	1820:1855	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	5	87	theme	increased	951:959	arg1	responses					982:990	increased anti-tumor cytotoxic responses	951:990	increased anti-tumor cytotoxic responses relative to controls	951:1011	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	7	88	theme	overall	1428:1434	arg1	sulfation					1436:1444	reduced overall sulfation	1420:1444	reduced overall sulfation	1420:1444	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	8	89	theme	T-cell	1840:1845	arg1	interface					1847:1855	the antigen/MHC-I - T-cell interface	1820:1855	the antigen/MHC-I - T-cell interface	1820:1855	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	5	90	theme	cytotoxic	972:980	arg1	responses					982:990	increased anti-tumor cytotoxic responses	951:990	increased anti-tumor cytotoxic responses relative to controls	951:1011	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	8	91	theme	antigen	1579:1585	arg1	presentation					1587:1598	antigen presentation	1579:1598	antigen presentation in the context of major histocompatibility complex class-I (MHC-I)	1579:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	92	theme	Lewis	781:785	arg1	carcinoma					792:800	Lewis lung carcinoma	781:800	Lewis lung carcinoma growth	781:807	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	0	93	theme	Cellular	11:18	arg1	Mechanisms					31:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms	0:40	Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting.					
31896526	5	94	theme	CD8+	931:934	arg1	cells					938:942	splenic CD8+ T cells	923:942	splenic CD8+ T cells	923:942	In mice deficient in a major DC HS proteoglycan (syndecan-4), splenic CD8+ T cells showed increased anti-tumor cytotoxic responses relative to controls.					
31896526	7	95	theme	glycan	1337:1342	arg1	analysis					1358:1365	glycan compositional analysis	1337:1365	glycan compositional analysis	1337:1365	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	3	96	theme	immune	458:463	arg1	responses					465:473	tumor antigen-specific immune responses	435:473	tumor antigen-specific immune responses	435:473	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	1	97	from	importance	127:136	arg1	immunity					159:166	cancer immunity	152:166	cancer immunity	152:166	While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking.					
31896526	3	98	theme	tumor	435:439	arg1	responses					465:473	tumor antigen-specific immune responses	435:473	tumor antigen-specific immune responses	435:473	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	8	99	theme	major	1618:1622	arg1	complex					1643:1649	major histocompatibility complex	1618:1649	major histocompatibility complex class-I (MHC-I)	1618:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	100	theme	increased	820:828	arg1	cells					854:858	increased tumor-associated CD8+ T cells	820:858	increased tumor-associated CD8+ T cells	820:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	3	101	theme	dendritic	489:497	arg1	DCs					506:508	DCs	506:508	DCs	506:508	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	3	101	theme	dendritic	489:497	arg1	cells					499:503	endogenous dendritic cells	478:503	endogenous dendritic cells (DCs)	478:509	Augmenting tumor antigen-specific immune responses by endogenous dendritic cells (DCs) is appealing from a specificity standpoint, but challenging.					
31896526	0	102	theme	Genetic	59:65	arg1	Targeting					80:88	Genetic Proteoglycan Targeting	59:88	Genetic Proteoglycan Targeting	59:88	Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting.					
31896526	6	103	theme	CD8+	1160:1163	arg1	cells					1167:1171	splenic CD8+ T cells	1152:1171	splenic CD8+ T cells	1152:1171	Studies examining Ndst1f/f CD11cCre + mutants revealed that mutation was associated with an increase in anti-tumor cytolysis using either splenic CD8+ T cells or tumor-infiltrating (TIL) CD8+ T cells purified ex-vivo, and tested in pooled effector-to-target cytolytic assays against tumor cells from respective animals.					
31896526	8	104	theme	complex	1643:1649	arg1	MHC-I					1660:1664	MHC-I	1660:1664	MHC-I	1660:1664	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	8	104	theme	complex	1643:1649	arg1	class-I					1651:1657	major histocompatibility complex class-I	1618:1657	major histocompatibility complex class-I (MHC-I)	1618:1665	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	4	105	theme	CD8+	847:850	arg1	cells					854:858	increased tumor-associated CD8+ T cells	820:858	increased tumor-associated CD8+ T cells	820:858	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	2	106	theme	mediated	334:341	arg1	immunity					343:350	T-cell mediated immunity	327:350	T-cell mediated immunity	327:350	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
31896526	4	107	theme	carcinoma	792:800	arg1	growth					802:807	Lewis lung carcinoma growth	781:807	Lewis lung carcinoma growth	781:807	Here, we show that restricting a heparan sulfate (HS) loss-of-function mutation in the HS sulfating enzyme Ndst1 to predominantly conventional DCs (Ndst1f/f CD11cCre+ mutation) results in marked inhibition of Lewis lung carcinoma growth along with increased tumor-associated CD8+ T cells.					
31896526	7	108	theme	wildtype	1548:1555	arg1	HS					1560:1561	wildtype DC HS	1548:1561	wildtype DC HS	1548:1561	On glycan compositional analysis, HS purified from Ndst1f/f CD11cCre + mutant DCs had reduced overall sulfation, including reduced sulfation of a tri-sulfated disaccharide species that was intriguingly abundant on wildtype DC HS.					
31896526	8	109	from	targeting	1952:1960	arg1	cancer					1985:1990	cancer	1985:1990	cancer	1985:1990	Interestingly, antigen presentation in the context of major histocompatibility complex class-I (MHC-I) was enhanced in mutant DCs, with more striking effects in the setting of HS under-sulfation, pointing to a likely regulatory role by sulfated glycans at the antigen/MHC-I - T-cell interface; and possibly future opportunities to improve antigen-specific T cell responses by immunologic targeting of HS proteoglycans in cancer.					
31896526	2	110	theme	immune-checkpoint	365:381	arg1	pathways					383:390	immune-checkpoint pathways	365:390	immune-checkpoint pathways	365:390	In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated immunity by targeting immune-checkpoint pathways, robust responses are uncommon.					
30409628	4	0	dep	Deglycosylation	532:546	arg1	allergenicity					560:572	enhanced TM allergenicity	548:572	enhanced TM allergenicity	548:572	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	6	1	gly	deglycosylated	983:996	arg1	TM					998:999	the deglycosylated TM	979:999	the deglycosylated TM	979:999	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	7	2	from	inhibition	1127:1136	arg1	proliferation					1161:1173	colon cell (Caco-2) proliferation	1141:1173	colon cell (Caco-2) proliferation	1141:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	5	3	theme	digests	804:810	arg1	allergenicity					787:799	allergenicity	787:799	allergenicity of digests	787:810	Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests.					
30409628	1	4	theme	human	340:344	arg1	sensitization					351:363	human cell sensitization	340:363	human cell sensitization	340:363	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	7	5	theme	IL-8	1191:1194	arg1	release					1196:1202	IL-8 release	1191:1202	IL-8 release	1191:1202	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	3	6	theme	other	495:499	arg1	structures					511:520	other secondary structures	495:520	other secondary structures	495:520	After deglycosylation and glycation, the α-helix of TM reduced, while other secondary structures promoted.					
30409628	1	7	dep	deglycosylation	233:247	arg1	enzyme					250:255	enzyme	250:255	enzyme	250:255	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	7	dep	deglycosylation	233:247	arg1	regents					270:276	chemical regents	261:276	chemical regents	261:276	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	7	8	theme	IL-8	1267:1270	arg1	release					1272:1278	IL-8 release	1267:1278	IL-8 release	1267:1278	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	1	9	theme	cell	346:349	arg1	sensitization					351:363	human cell sensitization	340:363	human cell sensitization	340:363	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	4	10	dep	digests	608:614	arg1	weakened					660:667	weakened	660:667	weakened the allergenicity and digestibility of TM	660:709	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	1	11	theme	chemical	261:268	arg1	regents					270:276	chemical regents	261:276	chemical regents	261:276	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	5	12	theme	Gastric	754:760	arg1	digestion					762:770	Gastric digestion	754:770	Gastric digestion	754:770	Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests.					
30409628	1	13	dep	conformation	308:319	arg1	the					304:306	the	304:306	the	304:306	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	4	14	theme	weaker	732:737	arg1	allergenicity					739:751	weaker allergenicity	732:751	weaker allergenicity	732:751	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	1	15	gly	glycoprotein	204:215	arg1	glycoprotein					204:215	glycoprotein	204:215	glycoprotein	204:215	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	16	gly	deglycosylation	233:247	arg1	cell					346:349	human cell sensitization	340:363	human cell sensitization	340:363	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	16	gly	deglycosylation	233:247	arg1	TM					368:369	TM	368:369	TM	368:369	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	6	17	theme	KU812	1085:1089	arg1	activation					1091:1100	TM weakened KU812 activation	1073:1100	TM weakened KU812 activation	1073:1100	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	5	18	theme	intestinal	813:822	arg1	digestion					824:832	intestinal digestion	813:832	intestinal digestion	813:832	Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests.					
30409628	1	19	theme	TM	368:369	arg1	allergenicity					322:334	allergenicity	322:334	allergenicity	322:334	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	19	theme	TM	368:369	arg1	conformation					308:319	conformation	308:319	conformation	308:319	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	19	theme	TM	368:369	arg1	sensitization					351:363	human cell sensitization	340:363	human cell sensitization	340:363	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	7	20	theme	cell	1147:1150	arg1	proliferation					1161:1173	colon cell (Caco-2) proliferation	1141:1173	colon cell (Caco-2) proliferation	1141:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	0	21	dep	Exopalaemon	85:95	arg1	modestus					97:104	Exopalaemon modestus	85:104	Exopalaemon modestus	85:104	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	6	22	theme	basophil	1032:1039	arg1	activation					1018:1027	stronger activation	1009:1027	stronger activation of basophil (KU812)	1009:1047	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	7	23	theme	Caco-2	1242:1247	arg1	proliferation					1249:1261	Caco-2 proliferation	1242:1261	Caco-2 proliferation	1242:1261	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	7	24	theme	glucose	1211:1217	arg1	TM					1228:1229	glucose glycated TM	1211:1229	glucose glycated TM	1211:1229	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	7	25	theme	Caco-2	1153:1158	arg1	proliferation					1161:1173	colon cell (Caco-2) proliferation	1141:1173	colon cell (Caco-2) proliferation	1141:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	4	26	with	digests	719:725	arg1	allergenicity					739:751	weaker allergenicity	732:751	weaker allergenicity	732:751	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	7	27	theme	glycated	1219:1226	arg1	TM					1228:1229	glucose glycated TM	1211:1229	glucose glycated TM	1211:1229	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	4	28	theme	TM	557:558	arg1	allergenicity					560:572	enhanced TM allergenicity	548:572	enhanced TM allergenicity	548:572	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	7	29	theme	colon	1141:1145	arg1	proliferation					1161:1173	colon cell (Caco-2) proliferation	1141:1173	colon cell (Caco-2) proliferation	1141:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	1	30	theme	Maillard	283:290	arg1	reaction					292:299	Maillard reaction	283:299	Maillard reaction	283:299	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	0	31	theme	deglycosylation	118:132	arg1	Effects					107:113	Effects	107:113	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.	0:155	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	32	from	Exopalaemon	85:95	arg1	allergenicity					14:26	allergenicity	14:26	allergenicity	14:26	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	32	from	Exopalaemon	85:95	arg1	Conformation					0:11	Conformation	0:11	Conformation	0:11	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	32	from	Exopalaemon	85:95	arg1	sensitization					51:63	human cell allergy sensitization	32:63	human cell allergy sensitization	32:63	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	4	33	theme	glucose	642:648	arg1	glycation					650:658	glucose glycation	642:658	glucose glycation	642:658	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	0	34	theme	cell	38:41	arg1	sensitization					51:63	human cell allergy sensitization	32:63	human cell allergy sensitization	32:63	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	1	35	theme	reaction	292:299	arg1	effects					222:228	the effects	218:228	the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM	218:369	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	0	36	theme	reaction	147:154	arg1	Effects					107:113	Effects	107:113	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.	0:155	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	1	37	dep	Exopalaemon	179:189	arg1	modestus					191:198	Exopalaemon modestus	179:198	Exopalaemon modestus	179:198	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	0	38	theme	human	32:36	arg1	sensitization					51:63	human cell allergy sensitization	32:63	human cell allergy sensitization	32:63	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	39	theme	Maillard	138:145	arg1	reaction					147:154	Maillard reaction	138:154	Maillard reaction	138:154	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	6	40	theme	TM	1073:1074	arg1	activation					1091:1100	TM weakened KU812 activation	1073:1100	TM weakened KU812 activation	1073:1100	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	7	41	theme	release	1196:1202	arg1	increase					1179:1186	increase	1179:1186	increase of IL-8 release	1179:1202	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	7	41	theme	release	1196:1202	arg1	inhibition					1127:1136	inhibition	1127:1136	inhibition on colon cell (Caco-2) proliferation	1127:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30409628	6	42	theme	stronger	1009:1016	arg1	activation					1018:1027	stronger activation	1009:1027	stronger activation of basophil (KU812)	1009:1047	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	2	43	contain	had	393:395	arg1	TM					390:391	TM	390:391	TM	390:391	TM had both N-glycan and O-glycan.					
30409628	2	43	contain	had	393:395	arg2	O-glycan					415:422	O-glycan	415:422	O-glycan	415:422	TM had both N-glycan and O-glycan.					
30409628	2	43	contain	had	393:395	arg2	N-glycan					402:409	N-glycan	402:409	N-glycan	402:409	TM had both N-glycan and O-glycan.					
30409628	5	44	theme	gastrointestinal	873:888	arg1	digestion					890:898	gastrointestinal digestion	873:898	gastrointestinal digestion	873:898	Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests.					
30409628	0	45	theme	allergy	43:49	arg1	sensitization					51:63	human cell allergy sensitization	32:63	human cell allergy sensitization	32:63	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	4	46	theme	enhanced	548:555	arg1	allergenicity					560:572	enhanced TM allergenicity	548:572	enhanced TM allergenicity	548:572	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	4	47	dep	allergenicity	673:685	arg1	the					669:671	the	669:671	the	669:671	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	1	48	from	effects	222:228	arg1	allergenicity					322:334	allergenicity	322:334	allergenicity	322:334	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	48	from	effects	222:228	arg1	conformation					308:319	conformation	308:319	conformation	308:319	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	48	from	effects	222:228	arg1	sensitization					351:363	human cell sensitization	340:363	human cell sensitization	340:363	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	4	49	theme	lower	621:625	arg1	allergenicity					627:639	lower allergenicity	621:639	lower allergenicity	621:639	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	1	50	from	Exopalaemon	179:189	arg1	Tropomyosin					157:167	Tropomyosin	157:167	Tropomyosin (TM) from Exopalaemon modestus	157:198	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	1	50	from	Exopalaemon	179:189	arg1	TM					170:171	TM	170:171	TM	170:171	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	3	51	theme	TM	477:478	arg1	α-helix					466:472	the α-helix	462:472	the α-helix of TM	462:478	After deglycosylation and glycation, the α-helix of TM reduced, while other secondary structures promoted.					
30409628	6	52	theme	weakened	1076:1083	arg1	activation					1091:1100	TM weakened KU812 activation	1073:1100	TM weakened KU812 activation	1073:1100	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	0	53	theme	tropomyosin	68:78	arg1	allergenicity					14:26	allergenicity	14:26	allergenicity	14:26	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	53	theme	tropomyosin	68:78	arg1	Conformation					0:11	Conformation	0:11	Conformation	0:11	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	0	53	theme	tropomyosin	68:78	arg1	sensitization					51:63	human cell allergy sensitization	32:63	human cell allergy sensitization	32:63	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	5	54	theme	digests	947:953	arg1	allergenicity					930:942	the allergenicity	926:942	the allergenicity of digests	926:953	Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests.					
30409628	3	55	theme	secondary	501:509	arg1	structures					511:520	other secondary structures	495:520	other secondary structures	495:520	After deglycosylation and glycation, the α-helix of TM reduced, while other secondary structures promoted.					
30409628	1	56	theme	deglycosylation	233:247	arg1	effects					222:228	the effects	218:228	the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM	218:369	Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated.					
30409628	6	57	theme	deglycosylated	983:996	arg1	TM					998:999	the deglycosylated TM	979:999	the deglycosylated TM	979:999	After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation.					
30409628	0	58	dep	Conformation	0:11	arg1	Effects					107:113	Effects	107:113	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.	0:155	Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.					
30409628	4	59	theme	TM	708:709	arg1	digestibility					691:703	digestibility	691:703	digestibility	691:703	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	4	59	theme	TM	708:709	arg1	allergenicity					673:685	allergenicity	673:685	allergenicity	673:685	Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity.					
30409628	7	60	from	increase	1179:1186	arg1	proliferation					1161:1173	colon cell (Caco-2) proliferation	1141:1173	colon cell (Caco-2) proliferation	1141:1173	Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.					
30030471	2	0	theme	liquid	343:348	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	0	theme	liquid	343:348	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	0	1	theme	extensive	89:97	arg1	glycan					99:104	extensive glycan and lipid changes	89:122	glycan	99:104	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	2	2	theme	performance	331:341	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	2	theme	performance	331:341	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	0	3	theme	lipid	110:114	arg1	changes					116:122	extensive glycan and lipid changes	89:122	changes	116:122	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	3	4	theme	GSL	692:694	arg1	portion					677:683	the ceramide portion	664:683	the ceramide portion of the GSL	664:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	5	5	from	time-points	887:897	arg1	samples					853:859	Caco-2 samples	846:859	Caco-2 samples from five differentiation time-points	846:897	A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points.					
30030471	2	6	theme	cell	545:548	arg1	lines					550:554	cultured cell lines	536:554	cultured cell lines	536:554	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	1	7	located	found	155:159	arg2	Glycosphingolipids					125:142	Glycosphingolipids	125:142	Glycosphingolipids (GSLs)	125:149	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	1	7	located	found	155:159	arg1	membranes					173:181	cellular membranes	164:181	cellular membranes of most organisms	164:199	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	1	7	located	found	155:159	arg2	GSLs					145:148	GSLs	145:148	GSLs	145:148	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	5	8	theme	differentiation	871:885	arg1	time-points					887:897	five differentiation time-points	866:897	five differentiation time-points	866:897	A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points.					
30030471	2	9	theme	cultured	536:543	arg1	lines					550:554	cultured cell lines	536:554	cultured cell lines	536:554	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	4	10	used	used	704:707	arg2	It					697:698	It	697:698	It	697:698	It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation.					
30030471	4	11	theme	cells	763:767	arg1	glycolipidome					739:751	the glycolipidome	735:751	the glycolipidome of Caco-2 cells	735:767	It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation.					
30030471	5	12	theme	Caco-2	846:851	arg1	samples					853:859	Caco-2 samples	846:859	Caco-2 samples from five differentiation time-points	846:897	A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points.					
30030471	2	13	theme	Chip-Q-TOF	424:433	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	13	theme	Chip-Q-TOF	424:433	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	14	theme	intact	487:492	arg1	GSLs					494:497	intact GSLs	487:497	intact GSLs	487:497	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	4	15	theme	Caco-2	756:761	arg1	cells					763:767	Caco-2 cells	756:767	Caco-2 cells	756:767	It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation.					
30030471	2	16	theme	nanoHPLC	415:422	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	16	theme	nanoHPLC	415:422	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	0	17	theme	glycosphingolipidomic	7:27	arg1	analysis					29:36	Intact glycosphingolipidomic analysis	0:36	Intact glycosphingolipidomic analysis of the cell membrane during differentiation	0:80	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	1	18	theme	most	186:189	arg1	organisms					191:199	most organisms	186:199	most organisms	186:199	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	0	19	theme	Intact	0:5	arg1	analysis					29:36	Intact glycosphingolipidomic analysis	0:36	Intact glycosphingolipidomic analysis of the cell membrane during differentiation	0:80	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	3	20	from	composition	599:609	arg1	portion					677:683	the ceramide portion	664:683	the ceramide portion of the GSL	664:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	1	21	theme	organisms	191:199	arg1	membranes					173:181	cellular membranes	164:181	cellular membranes of most organisms	164:199	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	6	22	theme	cell	964:967	arg1	profile					951:957	a comprehensive glycolipidomic profile	920:957	a comprehensive glycolipidomic profile of a cell	920:967	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	3	23	dep	composition	599:609	arg1	the					595:597	the	595:597	the	595:597	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	6	24	theme	structures	1037:1046	arg1	variation					1013:1021	the dynamic variation	1001:1021	the dynamic variation of intact GSL structures	1001:1046	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	1	25	theme	cellular	164:171	arg1	membranes					173:181	cellular membranes	164:181	cellular membranes of most organisms	164:199	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	3	26	from	sequence	615:622	arg1	portion					677:683	the ceramide portion	664:683	the ceramide portion of the GSL	664:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	5	27	theme	unique	817:822	arg1	GSLs					824:827	226 unique GSLs	813:827	226 unique GSLs	813:827	A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points.					
30030471	0	28	theme	cell	45:48	arg1	membrane					50:57	the cell membrane	41:57	the cell membrane	41:57	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	2	29	theme	animal	560:565	arg1	tissue					567:572	animal tissue	560:572	animal tissue	560:572	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	5	30	theme	GSLs	824:827	arg1	total					804:808	A total	802:808	A total of 226 unique GSLs	802:827	A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points.					
30030471	1	31	theme	important	210:218	arg1	roles					220:224	important roles	210:224	important roles	210:224	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	3	32	from	variations	650:659	arg1	portion					677:683	the ceramide portion	664:683	the ceramide portion of the GSL	664:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	6	33	theme	GSL	1033:1035	arg1	structures					1037:1046	intact GSL structures	1026:1046	intact GSL structures	1026:1046	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	6	34	theme	glycolipidomic	936:949	arg1	profile					951:957	a comprehensive glycolipidomic profile	920:957	a comprehensive glycolipidomic profile of a cell	920:967	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	2	35	theme	high	326:329	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	35	theme	high	326:329	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	0	36	theme	membrane	50:57	arg1	analysis					29:36	Intact glycosphingolipidomic analysis	0:36	Intact glycosphingolipidomic analysis of the cell membrane during differentiation	0:80	Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes.					
30030471	6	37	theme	comprehensive	922:934	arg1	profile					951:957	a comprehensive glycolipidomic profile	920:957	a comprehensive glycolipidomic profile of a cell	920:967	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	2	38	theme	nanoflow	317:324	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	38	theme	nanoflow	317:324	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	4	39	from	profile	712:718	arg1	glycolipidome					739:751	the glycolipidome	735:751	the glycolipidome of Caco-2 cells	735:767	It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation.					
30030471	6	40	theme	intact	1026:1031	arg1	structures					1037:1046	intact GSL structures	1026:1046	intact GSL structures	1026:1046	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
30030471	1	41	theme	cell-cell	229:237	arg1	recognition					239:249	cell-cell recognition	229:249	cell-cell recognition	229:249	Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others.					
30030471	3	42	theme	ceramide	668:675	arg1	portion					677:683	the ceramide portion	664:683	the ceramide portion of the GSL	664:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	2	43	theme	mass	396:399	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	43	theme	mass	396:399	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	44	theme	samples	517:523	arg1	samples					517:523	samples	517:523	samples	517:523	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	44	theme	samples	517:523	arg1	tissue					567:572	animal tissue	560:572	animal tissue	560:572	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	44	theme	samples	517:523	arg1	variety					506:512	a variety	504:512	a variety	504:512	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	44	theme	samples	517:523	arg1	lines					550:554	cultured cell lines	536:554	cultured cell lines	536:554	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	3	45	theme	glycan	631:636	arg1	variations					650:659	variations	650:659	variations in the ceramide portion of the GSL	650:694	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	3	45	theme	glycan	631:636	arg1	sequence					615:622	sequence	615:622	sequence	615:622	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	3	45	theme	glycan	631:636	arg1	composition					599:609	composition	599:609	composition	599:609	The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL.					
30030471	2	46	theme	chromatography-chip-quadrupole-time-of-flight	350:394	arg1	spectrometry					401:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry	317:412	nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS)	317:437	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	2	46	theme	chromatography-chip-quadrupole-time-of-flight	350:394	arg1	MS					435:436	nanoHPLC Chip-Q-TOF MS	415:436	nanoHPLC Chip-Q-TOF MS	415:436	A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue.					
30030471	6	47	theme	dynamic	1005:1011	arg1	variation					1013:1021	the dynamic variation	1001:1021	the dynamic variation of intact GSL structures	1001:1046	The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.					
29556960	12	0	theme	in	1900:1901	arg1	analyses					1919:1926	the in vitro and in vivo functional analyses	1883:1926	the in vitro and in vivo functional analyses	1883:1926	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	13	1	theme	PK	1929:1930	arg1	parameters					1932:1941	PK parameters	1929:1941	PK parameters of LBDE	1929:1949	PK parameters of LBDE were in good agreement with those of NESP®.					
29556960	8	2	theme	Non-reducing	1297:1308	arg1	RESULTS					1289:1295	RESULTS	1289:1295	RESULTS Non-reducing	1289:1308	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	8	3	theme	reducing	1314:1321	arg1	mapping					1337:1343	reducing Lys-C peptide mapping	1314:1343	reducing Lys-C peptide mapping	1314:1343	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	11	4	theme	minor	1784:1788	arg1	bands					1790:1794	no minor bands	1781:1794	no minor bands	1781:1794	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	3	5	theme	protein	584:590	arg1	structures					592:601	higher-order protein structures	571:601	higher-order protein structures	571:601	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	9	6	theme	relative	1584:1591	arg1	contents					1593:1600	their relative contents	1578:1600	their relative contents	1578:1600	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	8	7	theme	peptide	1329:1335	arg1	mapping					1337:1343	reducing Lys-C peptide mapping	1314:1343	reducing Lys-C peptide mapping	1314:1343	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	3	8	theme	peptide	629:635	arg1	mapping					637:643	Lys-C peptide mapping	623:643	Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy	623:778	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	14	9	theme	CONCLUSION	1995:2004	arg1	LBDE					2006:2009	CONCLUSION LBDE	1995:2009	CONCLUSION LBDE	1995:2009	CONCLUSION LBDE shows high similarity to NESP® with regard to structure and function.					
29556960	6	10	theme	binding	1109:1115	arg1	affinity					1117:1124	binding affinity	1109:1124	binding affinity for human erythropoietin receptor	1109:1158	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	0	11	theme	Originator	121:130	arg1	Alfa					144:147	Its Originator Darbepoetin Alfa	117:147	Its Originator Darbepoetin Alfa	117:147	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	0	11	theme	Originator	121:130	arg1	NESP®					150:154	NESP®	150:154	NESP®	150:154	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	5	12	theme	size-exclusion	918:931	arg1	SEC-HPLC					988:995	SEC-HPLC	988:995	SEC-HPLC	988:995	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	12	theme	size-exclusion	918:931	arg1	chromatography					972:985	size-exclusion chromatography-high-performance liquid chromatography	918:985	size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC)	918:996	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	0	13	theme	Alfa	144:147	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	3	14	theme	chromatography-mass	657:675	arg1	LC-MS					691:695	LC-MS	691:695	LC-MS	691:695	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	14	theme	chromatography-mass	657:675	arg1	spectrometry					677:688	liquid chromatography-mass spectrometry	650:688	liquid chromatography-mass spectrometry (LC-MS)	650:696	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	5	15	theme	liquid	965:970	arg1	SEC-HPLC					988:995	SEC-HPLC	988:995	SEC-HPLC	988:995	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	15	theme	liquid	965:970	arg1	chromatography					972:985	size-exclusion chromatography-high-performance liquid chromatography	918:985	size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC)	918:996	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	8	16	theme	peak	1369:1372	arg1	profile					1374:1380	a highly similar peak profile	1352:1380	a highly similar peak profile	1352:1380	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	8	17	theme	same	1423:1426	arg1	structure					1436:1444	the same primary structure	1419:1444	the same primary structure	1419:1444	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	5	18	theme	gel	1040:1042	arg1	SDS-PAGE					1061:1068	SDS-PAGE	1061:1068	SDS-PAGE	1061:1068	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	18	theme	gel	1040:1042	arg1	electrophoresis					1044:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis	1002:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	1002:1069	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	8	19	theme	disulfide	1450:1458	arg1	bonds					1460:1464	disulfide bonds	1450:1464	disulfide bonds	1450:1464	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	0	20	theme	Biosimilar	78:87	arg1	Alfa					101:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	5	21	theme	sodium	1002:1007	arg1	SDS-PAGE					1061:1068	SDS-PAGE	1061:1068	SDS-PAGE	1061:1068	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	21	theme	sodium	1002:1007	arg1	electrophoresis					1044:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis	1002:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	1002:1069	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	2	22	theme	biological	516:525	arg1	characterization					527:542	a comprehensive physicochemical and biological characterization	480:542	a comprehensive physicochemical and biological characterization study	480:548	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	0	23	theme	Alfa	101:104	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	5	24	theme	sulfate	1017:1023	arg1	SDS-PAGE					1061:1068	SDS-PAGE	1061:1068	SDS-PAGE	1061:1068	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	24	theme	sulfate	1017:1023	arg1	electrophoresis					1044:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis	1002:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	1002:1069	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	6	25	theme	erythropoietin	1136:1149	arg1	receptor					1151:1158	human erythropoietin receptor	1130:1158	human erythropoietin receptor	1130:1158	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	0	26	theme	LBDE	107:110	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	8	27	theme	primary	1428:1434	arg1	structure					1436:1444	the same primary structure	1419:1444	the same primary structure	1419:1444	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	6	28	dep	in	1194:1195	arg1	vivo					1197:1200	vivo	1197:1200	vivo	1197:1200	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	12	29	dep	in	1900:1901	arg1	vivo					1903:1906	vivo	1903:1906	vivo	1903:1906	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	1	30	dep	BACKGROUND	157:166	arg1	ensured					270:276	ensured	270:276	should be ensured through thorough physicochemical and biological characterization	260:341	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	2	31	theme	biosimilar	411:420	arg1	alfa					434:437	the proposed biosimilar darbepoetin alfa	398:437	the proposed biosimilar darbepoetin alfa	398:437	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	0	32	theme	Comprehensive	0:12	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	9	33	theme	Glycosylation	1467:1479	arg1	analyses					1493:1500	Glycosylation and isoform analyses	1467:1500	Glycosylation and isoform analyses	1467:1500	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	10	34	theme	indistinguishable	1654:1670	arg1	spectra					1672:1678	indistinguishable spectra	1654:1678	indistinguishable spectra	1654:1678	Spectroscopic analysis of LBDE showed indistinguishable spectra with NESP®.					
29556960	9	35	theme	isoform	1485:1491	arg1	analyses					1493:1500	Glycosylation and isoform analyses	1467:1500	Glycosylation and isoform analyses	1467:1500	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	12	36	from	difference	1858:1867	arg1	analyses					1919:1926	the in vitro and in vivo functional analyses	1883:1926	the in vitro and in vivo functional analyses	1883:1926	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	2	37	theme	physicochemical	496:510	arg1	characterization					527:542	a comprehensive physicochemical and biological characterization	480:542	a comprehensive physicochemical and biological characterization study	480:548	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	10	38	theme	LBDE	1642:1645	arg1	analysis					1630:1637	Spectroscopic analysis	1616:1637	Spectroscopic analysis of LBDE	1616:1645	Spectroscopic analysis of LBDE showed indistinguishable spectra with NESP®.					
29556960	14	39	theme	high	2017:2020	arg1	similarity					2022:2031	high similarity	2017:2031	high similarity to NESP® with regard to structure and function	2017:2078	CONCLUSION LBDE shows high similarity to NESP® with regard to structure and function.					
29556960	1	40	theme	biological	315:324	arg1	characterization					326:341	biological characterization	315:341	biological characterization	315:341	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	3	41	theme	disulfide	699:707	arg1	bond					709:712	disulfide bond	699:712	disulfide bond identification	699:727	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	11	42	theme	size	1740:1743	arg1	variants					1745:1752	size variants	1740:1752	size variants	1740:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	7	43	theme	injection	1271:1279	arg1	routes					1281:1286	two injection routes	1267:1286	two injection routes	1267:1286	Pharmacokinetics (PK) were evaluated using rats through two injection routes.					
29556960	4	44	theme	zone	862:865	arg1	electrophoresis					867:881	capillary zone electrophoresis	852:881	capillary zone electrophoresis	852:881	Glycosylation and isoform distribution were analyzed using MS, LC, and capillary zone electrophoresis.					
29556960	1	45	theme	product	227:233	arg1	comparability					197:209	the comparability	193:209	the comparability of a biosimilar product to an originator product	193:258	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	12	46	with	difference	1858:1867	arg1	NESP®					1874:1878	NESP®	1874:1878	NESP®	1874:1878	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	3	47	theme	circular	730:737	arg1	dichroism					739:747	circular dichroism	730:747	circular dichroism	730:747	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	48	theme	Primary	559:565	arg1	METHODS					551:557	METHODS	551:557	METHODS Primary	551:565	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	9	49	theme	O-glycan	1540:1547	arg1	N-glycan					1527:1534	the attached N-glycan and O-glycan structures	1514:1558	N-glycan	1527:1534	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	9	49	theme	O-glycan	1540:1547	arg1	structures					1549:1558	the attached N-glycan and O-glycan structures	1514:1558	structures	1549:1558	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	9	49	theme	O-glycan	1540:1547	arg1	same					1569:1572	same	1569:1572	same	1569:1572	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	8	50	contain	have	1414:1417	arg1	NESP®					1408:1412	NESP®	1408:1412	NESP®	1408:1412	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	8	50	contain	have	1414:1417	arg2	bonds					1460:1464	disulfide bonds	1450:1464	disulfide bonds	1450:1464	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	8	50	contain	have	1414:1417	arg2	structure					1436:1444	the same primary structure	1419:1444	the same primary structure	1419:1444	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	8	50	contain	have	1414:1417	arg1	LBDE					1399:1402	LBDE	1399:1402	LBDE	1399:1402	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	3	51	theme	fluorescence	754:765	arg1	spectroscopy					767:778	fluorescence spectroscopy	754:778	fluorescence spectroscopy	754:778	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	12	52	theme	in	1887:1888	arg1	analyses					1919:1926	the in vitro and in vivo functional analyses	1883:1926	the in vitro and in vivo functional analyses	1883:1926	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	3	53	theme	higher-order	571:582	arg1	structures					592:601	higher-order protein structures	571:601	higher-order protein structures	571:601	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	1	54	theme	regulatory	172:181	arg1	approval					183:190	regulatory approval	172:190	regulatory approval	172:190	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	3	55	theme	Lys-C	623:627	arg1	mapping					637:643	Lys-C peptide mapping	623:643	Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy	623:778	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	8	56	theme	Lys-C	1323:1327	arg1	mapping					1337:1343	reducing Lys-C peptide mapping	1314:1343	reducing Lys-C peptide mapping	1314:1343	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	0	57	theme	Darbepoetin	132:142	arg1	Alfa					144:147	Its Originator Darbepoetin Alfa	117:147	Its Originator Darbepoetin Alfa	117:147	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	0	57	theme	Darbepoetin	132:142	arg1	NESP®					150:154	NESP®	150:154	NESP®	150:154	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	3	58	theme	liquid	650:655	arg1	LC-MS					691:695	LC-MS	691:695	LC-MS	691:695	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	58	theme	liquid	650:655	arg1	spectrometry					677:688	liquid chromatography-mass spectrometry	650:688	liquid chromatography-mass spectrometry (LC-MS)	650:696	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	5	59	theme	chromatography-high-performance	933:963	arg1	SEC-HPLC					988:995	SEC-HPLC	988:995	SEC-HPLC	988:995	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	59	theme	chromatography-high-performance	933:963	arg1	chromatography					972:985	size-exclusion chromatography-high-performance liquid chromatography	918:985	size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC)	918:996	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	6	60	theme	Biological	1072:1081	arg1	characterization					1083:1098	Biological characterization	1072:1098	Biological characterization	1072:1098	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	5	61	theme	polyacrylamide	1025:1038	arg1	SDS-PAGE					1061:1068	SDS-PAGE	1061:1068	SDS-PAGE	1061:1068	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	61	theme	polyacrylamide	1025:1038	arg1	electrophoresis					1044:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis	1002:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	1002:1069	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	8	62	theme	similar	1361:1367	arg1	profile					1374:1380	a highly similar peak profile	1352:1380	a highly similar peak profile	1352:1380	RESULTS Non-reducing and reducing Lys-C peptide mapping showed a highly similar peak profile, confirming that LBDE and NESP® have the same primary structure and disulfide bonds.					
29556960	13	63	with	agreement	1964:1972	arg1	those					1979:1983	those	1979:1983	those	1979:1983	PK parameters of LBDE were in good agreement with those of NESP®.					
29556960	11	64	located	detected	1801:1808	arg2	bands					1790:1794	no minor bands	1781:1794	no minor bands	1781:1794	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	11	64	located	detected	1801:1808	arg1	SDS-PAGE					1813:1820	SDS-PAGE	1813:1820	SDS-PAGE	1813:1820	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	6	65	theme	cell	1170:1173	arg1	proliferation					1175:1187	in vitro cell proliferation	1161:1187	in vitro cell proliferation	1161:1187	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	4	66	theme	isoform	799:805	arg1	distribution					807:818	isoform distribution	799:818	isoform distribution	799:818	Glycosylation and isoform distribution were analyzed using MS, LC, and capillary zone electrophoresis.					
29556960	11	67	located	found	1758:1762	arg1	SEC-HPLC					1767:1774	SEC-HPLC	1767:1774	SEC-HPLC	1767:1774	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	11	67	located	found	1758:1762	arg2	variants					1745:1752	size variants	1740:1752	size variants	1740:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	11	67	located	found	1758:1762	arg2	amount					1730:1735	a very small amount	1717:1735	a very small amount of size variants	1717:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	0	68	theme	Darbepoetin	89:99	arg1	Alfa					101:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	6	69	theme	in	1161:1162	arg1	proliferation					1175:1187	in vitro cell proliferation	1161:1187	in vitro cell proliferation	1161:1187	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	2	70	theme	characterization	527:542	arg1	study					544:548	a comprehensive physicochemical and biological characterization study	480:548	a comprehensive physicochemical and biological characterization study	480:548	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	12	71	dep	in	1887:1888	arg1	vitro					1890:1894	vitro	1890:1894	vitro	1890:1894	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	5	72	theme	dodecyl	1009:1015	arg1	SDS-PAGE					1061:1068	SDS-PAGE	1061:1068	SDS-PAGE	1061:1068	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	5	72	theme	dodecyl	1009:1015	arg1	electrophoresis					1044:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis	1002:1058	sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	1002:1069	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	3	73	with	mapping	637:643	arg1	spectroscopy					767:778	fluorescence spectroscopy	754:778	fluorescence spectroscopy	754:778	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	73	with	mapping	637:643	arg1	LC-MS					691:695	LC-MS	691:695	LC-MS	691:695	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	73	with	mapping	637:643	arg1	identification					714:727	disulfide bond identification	699:727	disulfide bond identification	699:727	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	73	with	mapping	637:643	arg1	dichroism					739:747	circular dichroism	730:747	circular dichroism	730:747	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	3	73	with	mapping	637:643	arg1	spectrometry					677:688	liquid chromatography-mass spectrometry	650:688	liquid chromatography-mass spectrometry (LC-MS)	650:696	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	6	74	theme	human	1130:1134	arg1	receptor					1151:1158	human erythropoietin receptor	1130:1158	human erythropoietin receptor	1130:1158	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	6	75	dep	in	1161:1162	arg1	vitro					1164:1168	vitro	1164:1168	vitro	1164:1168	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	10	76	theme	Spectroscopic	1616:1628	arg1	analysis					1630:1637	Spectroscopic analysis	1616:1637	Spectroscopic analysis of LBDE	1616:1645	Spectroscopic analysis of LBDE showed indistinguishable spectra with NESP®.					
29556960	2	77	theme	darbepoetin	422:432	arg1	alfa					434:437	the proposed biosimilar darbepoetin alfa	398:437	the proposed biosimilar darbepoetin alfa	398:437	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	5	78	theme	Size	884:887	arg1	variants					889:896	Size variants	884:896	Size variants	884:896	Size variants were evaluated with size-exclusion chromatography-high-performance liquid chromatography (SEC-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).					
29556960	2	79	theme	proposed	402:409	arg1	alfa					434:437	the proposed biosimilar darbepoetin alfa	398:437	the proposed biosimilar darbepoetin alfa	398:437	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	0	80	theme	Biological	34:43	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	1	81	theme	thorough	286:293	arg1	physicochemical					295:309	thorough physicochemical	286:309	thorough physicochemical	286:309	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	6	82	theme	in	1194:1195	arg1	potency					1202:1208	in vivo potency	1194:1208	in vivo potency	1194:1208	Biological characterization included binding affinity for human erythropoietin receptor, in vitro cell proliferation, and in vivo potency.					
29556960	3	83	theme	bond	709:712	arg1	identification					714:727	disulfide bond identification	699:727	disulfide bond identification	699:727	METHODS Primary and higher-order protein structures were analyzed using Lys-C peptide mapping with liquid chromatography-mass spectrometry (LC-MS), disulfide bond identification, circular dichroism, and fluorescence spectroscopy.					
29556960	13	84	theme	LBDE	1946:1949	arg1	parameters					1932:1941	PK parameters	1929:1941	PK parameters of LBDE	1929:1949	PK parameters of LBDE were in good agreement with those of NESP®.					
29556960	13	85	theme	good	1959:1962	arg1	agreement					1964:1972	good agreement	1959:1972	good agreement with those of NESP®	1959:1992	PK parameters of LBDE were in good agreement with those of NESP®.					
29556960	2	86	theme	comprehensive	482:494	arg1	characterization					527:542	a comprehensive physicochemical and biological characterization	480:542	a comprehensive physicochemical and biological characterization study	480:548	OBJECTIVE To evaluate the biosimilarity between LBDE, the proposed biosimilar darbepoetin alfa, and NESP®, its originator, we performed a comprehensive physicochemical and biological characterization study.					
29556960	0	87	theme	Proposed	69:76	arg1	Alfa					101:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	the Proposed Biosimilar Darbepoetin Alfa	65:104	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	12	88	theme	functional	1908:1917	arg1	analyses					1919:1926	the in vitro and in vivo functional analyses	1883:1926	the in vitro and in vivo functional analyses	1883:1926	Furthermore, LBDE did not show any difference with NESP® in the in vitro and in vivo functional analyses.					
29556960	11	89	theme	small	1724:1728	arg1	amount					1730:1735	a very small amount	1717:1735	a very small amount of size variants	1717:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	11	89	theme	small	1724:1728	arg1	variants					1745:1752	size variants	1740:1752	size variants	1740:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	9	90	theme	attached	1518:1525	arg1	N-glycan					1527:1534	the attached N-glycan and O-glycan structures	1514:1558	N-glycan	1527:1534	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	9	90	theme	attached	1518:1525	arg1	structures					1549:1558	the attached N-glycan and O-glycan structures	1514:1558	structures	1549:1558	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	9	90	theme	attached	1518:1525	arg1	same					1569:1572	same	1569:1572	same	1569:1572	Glycosylation and isoform analyses showed that the attached N-glycan and O-glycan structures were the same and their relative contents were similar.					
29556960	4	91	theme	capillary	852:860	arg1	electrophoresis					867:881	capillary zone electrophoresis	852:881	capillary zone electrophoresis	852:881	Glycosylation and isoform distribution were analyzed using MS, LC, and capillary zone electrophoresis.					
29556960	1	92	theme	biosimilar	216:225	arg1	product					227:233	a biosimilar product	214:233	a biosimilar product	214:233	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
29556960	11	93	theme	variants	1745:1752	arg1	amount					1730:1735	a very small amount	1717:1735	a very small amount of size variants	1717:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	11	93	theme	variants	1745:1752	arg1	variants					1745:1752	size variants	1740:1752	size variants	1740:1752	For both LBDE and NESP®, a very small amount of size variants was found in SEC-HPLC, and no minor bands were detected in SDS-PAGE.					
29556960	0	94	theme	Physicochemical	14:28	arg1	Characterization					45:60	Comprehensive Physicochemical and Biological Characterization	0:60	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.	0:155	Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.					
29556960	1	95	theme	originator	241:250	arg1	product					252:258	an originator product	238:258	an originator product	238:258	BACKGROUND For regulatory approval, the comparability of a biosimilar product to an originator product should be ensured through thorough physicochemical and biological characterization.					
31584586	5	0	theme	colitis-associated	754:771	arg1	tumorigenesis					779:791	colitis-associated colon tumorigenesis	754:791	colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	754:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	11	1	theme	suppressed	1869:1878	arg1	inflammation					1888:1899	inflammation	1888:1899	inflammation	1888:1899	These compositional alterations induced by the NVPS were associated with the suppressed colonic inflammation and carcinogenesis.					
31584586	12	2	theme	prebiotic	2101:2109	arg1	potential					2068:2076	the potential	2064:2076	the potential of NVPS	2064:2084	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	12	2	theme	prebiotic	2101:2109	arg1	candidate					2111:2119	a promising prebiotic candidate	2089:2119	a promising prebiotic candidate for the prevention and treatment of colorectal cancer	2089:2173	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	3	3	from	nutrients	551:559	arg1	rich					543:546	rich	543:546	rich	543:546	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	1	4	theme	cancer	270:275	arg1	progression					244:254	progression	244:254	progression	244:254	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	1	4	theme	cancer	270:275	arg1	occurrence					229:238	occurrence	229:238	occurrence	229:238	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	5	5	theme	colon	773:777	arg1	tumorigenesis					779:791	colitis-associated colon tumorigenesis	754:791	colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	754:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	6	from	tumorigenesis	779:791	arg1	mice					796:799	mice	796:799	mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	796:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	1	7	theme	gut	183:185	arg1	microbiota					187:196	the gut microbiota	179:196	the gut microbiota	179:196	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	12	8	theme	promising	2091:2099	arg1	potential					2068:2076	the potential	2064:2076	the potential of NVPS	2064:2084	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	12	8	theme	promising	2091:2099	arg1	candidate					2111:2119	a promising prebiotic candidate	2089:2119	a promising prebiotic candidate for the prevention and treatment of colorectal cancer	2089:2173	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	5	9	theme	N.	728:729	arg1	NVPS					740:743	NVPS	740:743	NVPS	740:743	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	9	theme	N.	728:729	arg1	commune					731:737	N. commune	728:737	N. commune (NVPS)	728:744	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	12	10	theme	colorectal	2157:2166	arg1	cancer					2168:2173	colorectal cancer	2157:2173	colorectal cancer	2157:2173	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	0	11	attach	isolated	16:23	arg2	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides isolated from Nostoc commune Vaucher	0:51	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	0	11	attach	isolated	16:23	arg1	Vaucher					45:51	Vaucher	45:51	Vaucher	45:51	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	10	12	theme	acid-producing	1646:1659	arg1	genera					1661:1666	acetic acid-producing genera	1639:1666	acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia)	1639:1730	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	9	13	theme	AOM/DSS	1403:1409	arg1	treatment					1411:1419	the AOM/DSS treatment	1399:1419	the AOM/DSS treatment	1399:1419	The results demonstrated that the alterations in the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment were significantly reversed in response to the NVPS treatment.					
31584586	10	14	theme	NVPS	1773:1776	arg1	intervention					1778:1789	the NVPS intervention	1769:1789	the NVPS intervention	1769:1789	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	4	15	theme	commune	632:638	arg1	weight					619:624	the dry weight	611:624	the dry weight of N. commune	611:638	Particularly, polysaccharides account for 60% of the dry weight of N. commune.					
31584586	12	16	theme	NVPS	2081:2084	arg1	potential					2068:2076	the potential	2064:2076	the potential of NVPS	2064:2084	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	12	16	theme	NVPS	2081:2084	arg1	candidate					2111:2119	a promising prebiotic candidate	2089:2119	a promising prebiotic candidate for the prevention and treatment of colorectal cancer	2089:2173	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	9	17	theme	Bacteroidetes	1375:1387	arg1	abundance					1347:1355	the abundance	1343:1355	the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment	1343:1419	The results demonstrated that the alterations in the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment were significantly reversed in response to the NVPS treatment.					
31584586	10	18	dep	genera	1579:1584	arg1	Butyrivibrio					1603:1614	Butyrivibrio	1603:1614	Butyrivibrio	1603:1614	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	10	18	dep	genera	1579:1584	arg1	Butyricimonas					1620:1632	Butyricimonas	1620:1632	Butyricimonas	1620:1632	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	3	19	from	rich	543:546	arg1	nutrients					551:559	nutrients	551:559	nutrients	551:559	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	10	20	theme	acetic	1639:1644	arg1	genera					1661:1666	acetic acid-producing genera	1639:1666	acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia)	1639:1730	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	5	21	theme	sodium	853:858	arg1	DSS					866:868	dextran sulfate sodium salt (DSS)	837:869	dextran sulfate sodium salt (DSS)	837:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	0	22	theme	gut	121:123	arg1	microbiota					125:134	gut microbiota	121:134	gut microbiota	121:134	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	6	23	theme	intragastric	983:994	arg1	gavage					996:1001	an intragastric gavage	980:1001	an intragastric gavage for 14 weeks	980:1014	NVPS were administered to mice through an intragastric gavage for 14 weeks.					
31584586	7	24	theme	tumors	1119:1124	arg1	sizes					1110:1114	sizes	1110:1114	sizes	1110:1114	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	7	24	theme	tumors	1119:1124	arg1	number					1099:1104	number	1099:1104	number	1099:1104	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	7	25	theme	tumors	1166:1171	arg1	incidence					1142:1150	the incidence	1138:1150	the incidence of intestinal tumors	1138:1171	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	5	26	theme	salt	860:863	arg1	DSS					866:868	dextran sulfate sodium salt (DSS)	837:869	dextran sulfate sodium salt (DSS)	837:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	3	27	theme	Nostoc	422:427	arg1	commune					429:435	Nostoc commune	422:435	Nostoc commune Vaucher (N. commune)	422:456	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	2	28	theme	non-digestible	334:347	arg1	polysaccharides					349:363	non-digestible polysaccharides	334:363	non-digestible polysaccharides	334:363	Commonly known as a kind of constituent macromolecules, non-digestible polysaccharides have always been fermented by the intestinal microbiota.					
31584586	5	29	attach	isolated	714:721	arg2	polysaccharides					698:712	the polysaccharides	694:712	the polysaccharides isolated from N. commune (NVPS)	694:744	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	29	attach	isolated	714:721	arg1	NVPS					740:743	NVPS	740:743	NVPS	740:743	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	29	attach	isolated	714:721	arg1	commune					731:737	N. commune	728:737	N. commune (NVPS)	728:744	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	3	30	theme	commune	429:435	arg1	Vaucher					437:443	Nostoc commune Vaucher	422:443	Nostoc commune Vaucher (N. commune)	422:456	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	3	30	theme	commune	429:435	arg1	commune					449:455	N. commune	446:455	N. commune	446:455	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	5	31	theme	intestinal	918:927	arg1	microbiota					929:938	the intestinal microbiota	914:938	the intestinal microbiota	914:938	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	8	32	theme	bacterial	1231:1239	arg1	composition					1241:1251	the bacterial composition	1227:1251	the bacterial composition of the fecal samples obtained from mice	1227:1291	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	1	33	theme	important	207:215	arg1	role					217:220	an important role	204:220	an important role	204:220	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	0	34	theme	Nostoc	30:35	arg1	commune					37:43	Nostoc commune	30:43	Nostoc commune Vaucher	30:51	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	11	35	theme	compositional	1798:1810	arg1	alterations					1812:1822	These compositional alterations	1792:1822	These compositional alterations induced by the NVPS	1792:1842	These compositional alterations induced by the NVPS were associated with the suppressed colonic inflammation and carcinogenesis.					
31584586	2	36	theme	macromolecules	318:331	arg1	kind					298:301	a kind	296:301	a kind of constituent macromolecules	296:331	Commonly known as a kind of constituent macromolecules, non-digestible polysaccharides have always been fermented by the intestinal microbiota.					
31584586	8	37	theme	16S	1180:1182	arg1	sequencing					1194:1203	16S rRNA gene sequencing	1180:1203	16S rRNA gene sequencing	1180:1203	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	5	38	theme	polysaccharides	698:712	arg1	effects					683:689	the protective effects	668:689	the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	668:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	38	theme	polysaccharides	698:712	arg1	impact					879:884	the impact	875:884	the impact of these polysaccharides on the intestinal microbiota	875:938	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	2	39	theme	constituent	306:316	arg1	macromolecules					318:331	constituent macromolecules	306:331	constituent macromolecules	306:331	Commonly known as a kind of constituent macromolecules, non-digestible polysaccharides have always been fermented by the intestinal microbiota.					
31584586	3	40	theme	healthy	491:497	arg1	food					499:502	a healthy food	489:502	a healthy food	489:502	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	3	41	theme	N.	446:447	arg1	Vaucher					437:443	Nostoc commune Vaucher	422:443	Nostoc commune Vaucher (N. commune)	422:456	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	3	41	theme	N.	446:447	arg1	commune					449:455	N. commune	446:455	N. commune	446:455	Nostoc commune Vaucher (N. commune) has always been appreciated as a healthy food and supplementation worldwide as it is rich in nutrients.					
31584586	8	42	theme	fecal	1260:1264	arg1	samples					1266:1272	the fecal samples	1256:1272	the fecal samples obtained from mice	1256:1291	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	7	43	with	treatment	1051:1059	arg1	NVPS					1066:1069	NVPS	1066:1069	NVPS	1066:1069	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	5	44	theme	dextran	837:843	arg1	DSS					866:868	dextran sulfate sodium salt (DSS)	837:869	dextran sulfate sodium salt (DSS)	837:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	8	45	theme	gene	1189:1192	arg1	sequencing					1194:1203	16S rRNA gene sequencing	1180:1203	16S rRNA gene sequencing	1180:1203	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	0	46	from	tumorigenesis	86:98	arg1	mice					103:106	mice	103:106	mice	103:106	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	8	47	theme	rRNA	1184:1187	arg1	sequencing					1194:1203	16S rRNA gene sequencing	1180:1203	16S rRNA gene sequencing	1180:1203	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	12	48	theme	gut	2010:2012	arg1	profile					2025:2031	the gut microbiota profile	2006:2031	the gut microbiota profile altered by AOM/DSS	2006:2050	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	5	49	theme	sulfate	845:851	arg1	DSS					866:868	dextran sulfate sodium salt (DSS)	837:869	dextran sulfate sodium salt (DSS)	837:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	0	50	theme	colitis-associated	61:78	arg1	tumorigenesis					86:98	colitis-associated colon tumorigenesis	61:98	colitis-associated colon tumorigenesis in mice	61:106	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	10	51	theme	butyric	1556:1562	arg1	genera					1579:1584	butyric acid-producing genera	1556:1584	butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas)	1556:1633	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	11	52	theme	colonic	1880:1886	arg1	inflammation					1888:1899	inflammation	1888:1899	inflammation	1888:1899	These compositional alterations induced by the NVPS were associated with the suppressed colonic inflammation and carcinogenesis.					
31584586	8	53	theme	samples	1266:1272	arg1	composition					1241:1251	the bacterial composition	1227:1251	the bacterial composition of the fecal samples obtained from mice	1227:1291	Using 16S rRNA gene sequencing and qPCR, we analyzed the bacterial composition of the fecal samples obtained from mice.					
31584586	12	54	theme	appreciable	1964:1974	arg1	capability					1976:1985	an appreciable capability	1961:1985	an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS	1961:2050	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	5	55	theme	protective	672:681	arg1	effects					683:689	the protective effects	668:689	the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	668:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	9	56	from	alterations	1328:1338	arg1	abundance					1347:1355	the abundance	1343:1355	the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment	1343:1419	The results demonstrated that the alterations in the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment were significantly reversed in response to the NVPS treatment.					
31584586	1	57	theme	Accumulating	137:148	arg1	evidences					150:158	Accumulating evidences	137:158	Accumulating evidences	137:158	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	10	58	dep	genera	1661:1666	arg1	UCG					1685:1687	UCG	1685:1687	UCG	1685:1687	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	10	58	dep	genera	1661:1666	arg1	UCG					1710:1712	UCG	1710:1712	UCG	1710:1712	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	10	58	dep	genera	1661:1666	arg1	Blautia					1723:1729	Blautia	1723:1729	Blautia	1723:1729	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	12	59	dep	prevention	2129:2138	arg1	the					2125:2127	the	2125:2127	the	2125:2127	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	10	60	theme	short-chain	1498:1508	arg1	SCFA					1522:1525	SCFA	1522:1525	SCFA	1522:1525	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	10	60	theme	short-chain	1498:1508	arg1	acid					1516:1519	the short-chain fatty acid	1494:1519	the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia),	1494:1731	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	7	61	theme	intestinal	1155:1164	arg1	tumors					1166:1171	intestinal tumors	1155:1171	intestinal tumors	1155:1171	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	12	62	theme	cancer	2168:2173	arg1	treatment					2144:2152	treatment	2144:2152	treatment	2144:2152	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	12	62	theme	cancer	2168:2173	arg1	prevention					2129:2138	prevention	2129:2138	prevention	2129:2138	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	5	63	from	impact	879:884	arg1	microbiota					929:938	the intestinal microbiota	914:938	the intestinal microbiota	914:938	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	64	theme	polysaccharides	895:909	arg1	effects					683:689	the protective effects	668:689	the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS)	668:869	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	5	64	theme	polysaccharides	895:909	arg1	impact					879:884	the impact	875:884	the impact of these polysaccharides on the intestinal microbiota	875:938	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	9	65	theme	Firmicutes	1360:1369	arg1	abundance					1347:1355	the abundance	1343:1355	the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment	1343:1419	The results demonstrated that the alterations in the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment were significantly reversed in response to the NVPS treatment.					
31584586	4	66	theme	weight	619:624	arg1	weight					619:624	the dry weight	611:624	the dry weight of N. commune	611:638	Particularly, polysaccharides account for 60% of the dry weight of N. commune.					
31584586	4	66	theme	weight	619:624	arg1	%					606:606	60%	604:606	60% of the dry weight of N. commune	604:638	Particularly, polysaccharides account for 60% of the dry weight of N. commune.					
31584586	7	67	dep	number	1099:1104	arg1	the					1095:1097	the	1095:1097	the	1095:1097	Our results demonstrated that the treatment with NVPS significantly decreased the number and sizes of tumors and reduced the incidence of intestinal tumors.					
31584586	12	68	theme	microbiota	2014:2023	arg1	profile					2025:2031	the gut microbiota profile	2006:2031	the gut microbiota profile altered by AOM/DSS	2006:2050	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	1	69	dep	occurrence	229:238	arg1	the					225:227	the	225:227	the	225:227	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31584586	4	70	theme	dry	615:617	arg1	weight					619:624	the dry weight	611:624	the dry weight of N. commune	611:638	Particularly, polysaccharides account for 60% of the dry weight of N. commune.					
31584586	9	71	theme	NVPS	1468:1471	arg1	treatment					1473:1481	the NVPS treatment	1464:1481	the NVPS treatment	1464:1481	The results demonstrated that the alterations in the abundance of Firmicutes and Bacteroidetes caused by the AOM/DSS treatment were significantly reversed in response to the NVPS treatment.					
31584586	12	72	theme	NVPS	1990:1993	arg1	capability					1976:1985	an appreciable capability	1961:1985	an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS	1961:2050	In conclusion, our results demonstrated an appreciable capability of NVPS to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of NVPS as a promising prebiotic candidate for the prevention and treatment of colorectal cancer.					
31584586	2	73	theme	intestinal	399:408	arg1	microbiota					410:419	the intestinal microbiota	395:419	the intestinal microbiota	395:419	Commonly known as a kind of constituent macromolecules, non-digestible polysaccharides have always been fermented by the intestinal microbiota.					
31584586	10	74	theme	fatty	1510:1514	arg1	SCFA					1522:1525	SCFA	1522:1525	SCFA	1522:1525	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	10	74	theme	fatty	1510:1514	arg1	acid					1516:1519	the short-chain fatty acid	1494:1519	the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia),	1494:1731	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	4	75	theme	N.	629:630	arg1	commune					632:638	N. commune	629:638	N. commune	629:638	Particularly, polysaccharides account for 60% of the dry weight of N. commune.					
31584586	0	76	theme	colon	80:84	arg1	tumorigenesis					86:98	colitis-associated colon tumorigenesis	61:98	colitis-associated colon tumorigenesis in mice	61:106	Polysaccharides isolated from Nostoc commune Vaucher inhibit colitis-associated colon tumorigenesis in mice and modulate gut microbiota.					
31584586	10	77	theme	acid-producing	1564:1577	arg1	genera					1579:1584	butyric acid-producing genera	1556:1584	butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas)	1556:1633	Moreover, the short-chain fatty acid (SCFA)-producing genera, including butyric acid-producing genera (Butyricicoccus, Butyrivibrio and Butyricimonas) and acetic acid-producing genera (Lachnospiraceae UCG 001, Lachnospiraceae UCG 006, and Blautia), were drastically enriched following the NVPS intervention.					
31584586	5	78	from	effects	683:689	arg1	microbiota					929:938	the intestinal microbiota	914:938	the intestinal microbiota	914:938	In this study, we examined the protective effects of the polysaccharides isolated from N. commune (NVPS) against colitis-associated colon tumorigenesis in mice treated with azoxymethane (AOM) and dextran sulfate sodium salt (DSS) and the impact of these polysaccharides on the intestinal microbiota.					
31584586	1	79	theme	colorectal	259:268	arg1	cancer					270:275	colorectal cancer	259:275	colorectal cancer	259:275	Accumulating evidences have reported that the gut microbiota plays an important role in the occurrence and progression of colorectal cancer.					
31505886	4	0	theme	elevated	903:910	arg1	concentrations					912:925	elevated concentrations	903:925	elevated concentrations of yeast-derived polysaccharides	903:958	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	4	1	theme	wine	1113:1116	arg1	matrix					1118:1123	the wine matrix	1109:1123	the wine matrix	1109:1123	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	2	2	from	variables	582:590	arg1	particular					596:605	particular	596:605	particular	596:605	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	6	3	theme	wine	1516:1519	arg1	data					1535:1538	the wine compositional data	1512:1538	the wine compositional data	1512:1538	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	1	4	theme	wine	262:265	arg1	properties					275:284	wine sensory properties	262:284	wine sensory properties	262:284	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	8	5	theme	previous	1790:1797	arg1	reports					1799:1805	previous reports	1790:1805	previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes	1790:1906	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	4	6	link	yeast-derived	930:942	arg1	polysaccharides					944:958	yeast-derived polysaccharides	930:958	yeast-derived polysaccharides	930:958	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	6	7	theme	months	1313:1318	arg1	ageing					1320:1325	24 months ageing	1310:1325	24 months ageing	1310:1325	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	3	8	theme	polysaccharides	778:792	arg1	concentration					731:743	the highest concentration	719:743	the highest concentration of both yeast- and grape-derived polysaccharides	719:792	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	2	9	theme	wine	563:566	arg1	variables					582:590	wine compositional variables	563:590	wine compositional variables	563:590	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	5	10	theme	tannin	1131:1136	arg1	concentration					1138:1150	Wine tannin concentration	1126:1150	Wine tannin concentration	1126:1150	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	1	11	theme	yeast	225:229	arg1	strains					231:237	Saccharomyces cerevisiae yeast strains	200:237	Saccharomyces cerevisiae yeast strains	200:237	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	6	12	theme	wines	1298:1302	arg1	analysis					1282:1289	Sensory analysis	1274:1289	Sensory analysis of the wines after 24 months ageing	1274:1325	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	6	13	theme	squares	1487:1493	arg1	regression					1495:1504	partial least squares regression	1473:1504	partial least squares regression using the wine compositional data	1473:1538	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	8	14	theme	strain	1846:1851	arg1	choice					1830:1835	the choice	1826:1835	the choice of yeast strain	1826:1851	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	6	15	dep	attributes	1400:1409	arg1	opacity					1411:1417	opacity	1411:1417	opacity (visual colour)	1411:1433	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	6	15	dep	attributes	1400:1409	arg1	colour					1427:1432	visual colour	1420:1432	visual colour	1420:1432	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	6	15	dep	attributes	1400:1409	arg1	astringency					1439:1449	astringency	1439:1449	astringency	1439:1449	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	6	15	dep	attributes	1400:1409	arg1	attributes					1400:1409	only the attributes opacity (visual colour) and astringency	1391:1449	only the attributes opacity (visual colour) and astringency	1391:1449	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	4	16	theme	polysaccharides	944:958	arg1	concentrations					912:925	elevated concentrations	903:925	elevated concentrations of yeast-derived polysaccharides	903:958	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	2	17	theme	variables	582:590	arg1	number					553:558	the number	549:558	the number of wine compositional variables, in particular the concentration of tannin and polysaccharide	549:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	5	18	contain	having	1215:1220	arg1	L2323					1209:1213	L2323	1209:1213	L2323 having the highest	1209:1232	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	5	18	contain	having	1215:1220	arg2	highest					1226:1232	highest	1226:1232	highest	1226:1232	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	6	19	theme	Sensory	1274:1280	arg1	analysis					1282:1289	Sensory analysis	1274:1289	Sensory analysis of the wines after 24 months ageing	1274:1325	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	1	20	theme	Shiraz	424:429	arg1	wines					431:435	Shiraz wines	424:435	Shiraz wines	424:435	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	3	21	link	grape-derived	764:776	arg1	polysaccharides					778:792	both yeast- and grape-derived polysaccharides	748:792	both yeast- and grape-derived polysaccharides	748:792	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	8	22	theme	wine	1888:1891	arg1	outcomes					1899:1906	shape wine style outcomes	1882:1906	shape wine style outcomes	1882:1906	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	4	23	theme	mannoprotein	1052:1063	arg1	production					1065:1074	mannoprotein production	1052:1074	mannoprotein production	1052:1074	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	0	24	theme	Polysaccharide	89:102	arg1	Composition					104:114	Polysaccharide Composition	89:114	Polysaccharide Composition	89:114	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	1	25	from	knowledge	175:183	arg1	hybrids					250:256	their hybrids	244:256	their hybrids	244:256	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	1	25	from	knowledge	175:183	arg1	role					192:195	the role	188:195	the role of Saccharomyces cerevisiae yeast strains	188:237	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	1	25	from	knowledge	175:183	arg1	properties					275:284	wine sensory properties	262:284	wine sensory properties	262:284	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	4	26	contain	have	898:901	arg2	concentrations					912:925	elevated concentrations	903:925	elevated concentrations of yeast-derived polysaccharides	903:958	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	4	26	contain	have	898:901	arg1	Wines					795:799	Wines	795:799	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides)	795:888	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	7	27	theme	polysaccharide	1637:1650	arg1	concentrations					1603:1616	higher concentrations	1596:1616	higher concentrations of both tannin and polysaccharide	1596:1650	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	4	28	theme	corresponding	1001:1013	arg1	fermentations					1021:1033	corresponding model fermentations	1001:1033	corresponding model fermentations	1001:1033	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	5	29	with	strains	1195:1201	arg1	L2323					1209:1213	L2323	1209:1213	L2323 having the highest	1209:1232	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	6	30	theme	visual	1420:1425	arg1	opacity					1411:1417	opacity	1411:1417	opacity (visual colour)	1411:1433	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	6	30	theme	visual	1420:1425	arg1	colour					1427:1432	visual colour	1420:1432	visual colour	1420:1432	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	5	31	theme	high	1161:1164	arg1	level					1166:1170	a high level	1159:1170	a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration	1159:1271	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	7	32	theme	tannin	1626:1631	arg1	concentrations					1603:1616	higher concentrations	1596:1616	higher concentrations of both tannin and polysaccharide	1596:1650	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	0	33	theme	Commercial	0:9	arg1	Strains					42:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	2	34	theme	Significant	438:448	arg1	differences					450:460	Significant differences	438:460	Significant differences	438:460	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	2	35	from	particular	596:605	arg1	number					553:558	the number	549:558	the number of wine compositional variables, in particular the concentration of tannin and polysaccharide	549:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	6	36	theme	least	1481:1485	arg1	squares					1487:1493	partial least squares	1473:1493	partial least squares regression using the wine compositional data	1473:1538	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	4	37	theme	high	858:861	arg1	levels					863:868	high levels	858:868	high levels of polysaccharides	858:887	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	1	38	theme	yeast	313:317	arg1	strains					319:325	10 commercially available yeast strains	287:325	10 commercially available yeast strains	287:325	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	2	39	theme	post-alcoholic	475:488	arg1	fermentation					490:501	post-alcoholic fermentation	475:501	post-alcoholic fermentation	475:501	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	4	40	theme	polysaccharides	873:887	arg1	levels					863:868	high levels	858:868	high levels of polysaccharides	858:887	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	4	41	theme	strain	838:843	arg1	HPS					853:855	the mannoprotein-producing strain Uvaferm HPS	811:855	the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides)	811:888	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	5	42	with	concentration	1259:1271	arg1	L2323					1209:1213	L2323	1209:1213	L2323 having the highest	1209:1232	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	7	43	from	reports	1665:1671	arg1	literature					1680:1689	the literature	1676:1689	the literature which suggested that polysaccharide exerts a moderating effect on astringency	1676:1767	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	7	44	theme	astringency	1554:1564	arg1	attribute					1566:1574	the astringency attribute	1550:1574	the astringency attribute	1550:1574	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	0	45	with	Impact	64:69	arg1	Implications					121:132	Implications	121:132	Implications for Wine Colour and Astringency	121:164	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	3	46	theme	highest	723:729	arg1	concentration					731:743	the highest concentration	719:743	the highest concentration of both yeast- and grape-derived polysaccharides	719:792	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	6	47	theme	compositional	1521:1533	arg1	data					1535:1538	the wine compositional data	1512:1538	the wine compositional data	1512:1538	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	5	48	theme	lowest	1252:1257	arg1	concentration					1259:1271	the lowest concentration	1248:1271	AWRI1503 the lowest concentration	1239:1271	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	6	49	theme	significant	1336:1346	arg1	differences					1348:1358	significant differences	1336:1358	significant differences between the yeast strains	1336:1384	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	1	50	theme	sensory	267:273	arg1	properties					275:284	wine sensory properties	262:284	wine sensory properties	262:284	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	3	51	theme	yeast-	753:758	arg1	polysaccharides					778:792	both yeast- and grape-derived polysaccharides	748:792	both yeast- and grape-derived polysaccharides	748:792	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	4	52	dep	HPS	853:855	arg1	levels					863:868	high levels	858:868	high levels of polysaccharides	858:887	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	2	53	theme	polysaccharide	639:652	arg1	concentration					611:623	the concentration	607:623	particular the concentration of tannin and polysaccharide	596:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	2	54	theme	tannin	628:633	arg1	concentration					611:623	the concentration	607:623	particular the concentration of tannin and polysaccharide	596:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	1	55	theme	cerevisiae	214:223	arg1	strains					231:237	Saccharomyces cerevisiae yeast strains	200:237	Saccharomyces cerevisiae yeast strains	200:237	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	3	56	theme	grape-derived	764:776	arg1	polysaccharides					778:792	both yeast- and grape-derived polysaccharides	748:792	both yeast- and grape-derived polysaccharides	748:792	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	1	57	theme	strains	231:237	arg1	hybrids					250:256	their hybrids	244:256	their hybrids	244:256	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	1	57	theme	strains	231:237	arg1	role					192:195	the role	188:195	the role of Saccharomyces cerevisiae yeast strains	188:237	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	2	58	dep	particular	596:605	arg1	concentration					611:623	the concentration	607:623	particular the concentration of tannin and polysaccharide	596:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	8	59	theme	yeast	1840:1844	arg1	strain					1846:1851	yeast strain	1840:1851	yeast strain	1840:1851	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	4	60	theme	yeast-derived	930:942	arg1	polysaccharides					944:958	yeast-derived polysaccharides	930:958	yeast-derived polysaccharides	930:958	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	6	61	theme	yeast	1372:1376	arg1	strains					1378:1384	the yeast strains	1368:1384	the yeast strains	1368:1384	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	2	62	theme	compositional	568:580	arg1	variables					582:590	wine compositional variables	563:590	wine compositional variables	563:590	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	5	63	theme	Wine	1126:1129	arg1	concentration					1138:1150	Wine tannin concentration	1126:1150	Wine tannin concentration	1126:1150	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	8	64	theme	shape	1882:1886	arg1	outcomes					1899:1906	shape wine style outcomes	1882:1906	shape wine style outcomes	1882:1906	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	1	65	used	used	408:411	arg2	strains					319:325	10 commercially available yeast strains	287:325	10 commercially available yeast strains	287:325	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	1	66	theme	novel	387:391	arg1	properties					393:402	novel properties	387:402	novel properties	387:402	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	8	67	theme	style	1893:1897	arg1	outcomes					1899:1906	shape wine style outcomes	1882:1906	shape wine style outcomes	1882:1906	The results confirm previous reports demonstrating that the choice of yeast strain represents an opportunity to shape wine style outcomes.					
31505886	0	68	theme	Wine	138:141	arg1	Colour					143:148	Wine Colour	138:148	Wine Colour	138:148	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	1	69	theme	widespread	363:372	arg1	usage					374:378	their widespread usage	357:378	their widespread usage	357:378	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	2	70	theme	ageing	526:531	arg1	months					516:521	24 months	513:521	24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide	513:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	2	70	theme	ageing	526:531	arg1	fermentation					490:501	post-alcoholic fermentation	475:501	post-alcoholic fermentation	475:501	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	0	71	theme	Saccharomyces	11:23	arg1	Strains					42:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	5	72	theme	variability	1175:1185	arg1	level					1166:1170	a high level	1159:1170	a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration	1159:1271	Wine tannin concentration showed a high level of variability between strains, with L2323 having the highest, and AWRI1503 the lowest concentration.					
31505886	2	73	with	ageing	526:531	arg1	regards					538:544	regards	538:544	regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide	538:652	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	0	74	theme	Yeast	36:40	arg1	Strains					42:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains	0:48	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	6	75	theme	partial	1473:1479	arg1	squares					1487:1493	partial least squares	1473:1493	partial least squares regression using the wine compositional data	1473:1538	Sensory analysis of the wines after 24 months ageing revealed significant differences between the yeast strains, but only the attributes opacity (visual colour) and astringency could be predicted by partial least squares regression using the wine compositional data.					
31505886	1	76	theme	available	303:311	arg1	strains					319:325	10 commercially available yeast strains	287:325	10 commercially available yeast strains	287:325	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	7	77	theme	moderating	1736:1745	arg1	effect					1747:1752	a moderating effect	1734:1752	a moderating effect	1734:1752	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	1	78	dep	usage	374:378	arg1	basis					348:352	basis	348:352	basis	348:352	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	1	78	dep	usage	374:378	arg1	the					344:346	the	344:346	the	344:346	To gain knowledge on the role of Saccharomyces cerevisiae yeast strains (and their hybrids) on wine sensory properties, 10 commercially available yeast strains were selected on the basis of their widespread usage and/or novel properties and used to produce Shiraz wines.					
31505886	3	79	theme	Strain	655:660	arg1	L2323					662:666	Strain L2323	655:666	Strain L2323	655:666	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	4	80	theme	model	1015:1019	arg1	fermentations					1021:1033	corresponding model fermentations	1001:1033	corresponding model fermentations	1001:1033	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	0	81	theme	Shiraz	71:76	arg1	Tannin					78:83	Shiraz Tannin	71:83	Shiraz Tannin	71:83	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	7	82	theme	higher	1596:1601	arg1	concentrations					1603:1616	higher concentrations	1596:1616	higher concentrations of both tannin and polysaccharide	1596:1650	Notably, the astringency attribute was associated with higher concentrations of both tannin and polysaccharide, contrary to reports in the literature which suggested that polysaccharide exerts a moderating effect on astringency.					
31505886	3	83	theme	pectinolytic	685:696	arg1	activity					698:705	its pectinolytic activity	681:705	its pectinolytic activity	681:705	Strain L2323 is known for its pectinolytic activity and yielded the highest concentration of both yeast- and grape-derived polysaccharides.					
31505886	2	84	from	number	553:558	arg1	particular					596:605	particular	596:605	particular	596:605	Significant differences were evident post-alcoholic fermentation and after 24 months of ageing with regards to the number of wine compositional variables, in particular the concentration of tannin and polysaccharide.					
31505886	4	85	theme	mannoprotein-producing	815:836	arg1	HPS					853:855	the mannoprotein-producing strain Uvaferm HPS	811:855	the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides)	811:888	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
31505886	0	86	dep	Saccharomyces	11:23	arg1	cerevisiae					25:34	cerevisiae	25:34	cerevisiae	25:34	Commercial Saccharomyces cerevisiae Yeast Strains Significantly Impact Shiraz Tannin and Polysaccharide Composition with Implications for Wine Colour and Astringency.					
31505886	4	87	theme	Uvaferm	845:851	arg1	HPS					853:855	the mannoprotein-producing strain Uvaferm HPS	811:855	the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides)	811:888	Wines made with the mannoprotein-producing strain Uvaferm HPS (high levels of polysaccharides) did not have elevated concentrations of yeast-derived polysaccharides, despite this observation being made for corresponding model fermentations, suggesting that mannoprotein production or retention might be limited by the wine matrix.					
30637588	1	0	theme	biological	397:406	arg1	processes					408:416	major biological processes	391:416	major biological processes	391:416	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	3	1	theme	native	758:763	arg1	composition					774:784	the native ECM-like composition	754:784	the native ECM-like composition	754:784	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	6	2	theme	cell-derived	1345:1356	arg1	ECM					1358:1360	the different cell-derived ECM	1331:1360	the different cell-derived ECM	1331:1360	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	6	3	link	cell-derived	1345:1356	arg1	ECM					1358:1360	the different cell-derived ECM	1331:1360	the different cell-derived ECM	1331:1360	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	8	4	theme	novel	1786:1790	arg1	biomaterials					1801:1812	novel ECM-like biomaterials	1786:1812	novel ECM-like biomaterials for tissue engineering applications	1786:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	1	5	theme	proteins	175:182	arg1	matrix					118:123	The extracellular matrix	100:123	The extracellular matrix (ECM)	100:129	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	5	theme	proteins	175:182	arg1	meshwork					163:170	a highly dynamic and complex meshwork	134:170	a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs)	134:212	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	8	6	theme	biomaterials	1801:1812	arg1	development					1771:1781	the development	1767:1781	the development of novel ECM-like biomaterials for tissue engineering applications	1767:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	1	7	theme	glycosaminoglycans	188:205	arg1	matrix					118:123	The extracellular matrix	100:123	The extracellular matrix (ECM)	100:129	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	7	theme	glycosaminoglycans	188:205	arg1	meshwork					163:170	a highly dynamic and complex meshwork	134:170	a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs)	134:212	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	8	8	theme	tissue	1818:1823	arg1	applications					1837:1848	tissue engineering applications	1818:1848	tissue engineering applications	1818:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	1	9	theme	major	391:395	arg1	processes					408:416	major biological processes	391:416	major biological processes	391:416	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	3	10	theme	fabricated	620:629	arg1	ECM					644:646	in vitro fabricated cell-derived ECM	611:646	in vitro fabricated cell-derived ECM	611:646	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	4	11	theme	molecular	937:945	arg1	composition					974:984	GAG composition	970:984	GAG composition	970:984	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	8	12	theme	novel	1531:1535	arg1	approach					1537:1544	a novel approach	1529:1544	a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses	1529:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	6	13	theme	sulfation	1294:1302	arg1	patterns					1304:1311	disaccharide sulfation patterns	1281:1311	disaccharide sulfation patterns	1281:1311	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	7	14	theme	disaccharide	1431:1442	arg1	composition					1453:1463	the GAG and disaccharide relative composition	1419:1463	composition	1453:1463	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	3	15	theme	pathogen	845:852	arg1	risks					863:867	pathogen transfer risks	845:867	pathogen transfer risks	845:867	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	3	16	theme	scaffolds	891:899	arg1	availability					828:839	availability	828:839	availability	828:839	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	3	16	theme	scaffolds	891:899	arg1	risks					863:867	pathogen transfer risks	845:867	pathogen transfer risks	845:867	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	2	17	theme	pathways	513:520	arg1	modulators					489:498	modulators	489:498	modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation	489:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	6	18	theme	GAG	1260:1262	arg1	compositions					1264:1275	Distinct GAG compositions	1251:1275	Distinct GAG compositions	1251:1275	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	3	19	theme	availability	828:839	arg1	limitations					813:823	the limitations	809:823	the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds	809:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	0	20	theme	extracellular	76:88	arg1	matrices					90:97	cell-derived extracellular matrices	63:97	cell-derived extracellular matrices	63:97	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	5	21	theme	sulfation	1149:1157	arg1	patterns					1159:1166	sulfation patterns	1149:1166	sulfation patterns	1149:1166	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	3	22	theme	tissue-derived	872:885	arg1	scaffolds					891:899	tissue-derived ECM scaffolds	872:899	tissue-derived ECM scaffolds	872:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	1	23	with	meshwork	163:170	arg1	role					229:232	a crucial role	219:232	a crucial role in tissue homeostasis and organization	219:271	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	0	24	from	Compositional	0:12	arg1	matrices					90:97	cell-derived extracellular matrices	63:97	cell-derived extracellular matrices	63:97	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	3	25	theme	risks	863:867	arg1	limitations					813:823	the limitations	809:823	the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds	809:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	5	26	theme	content	1124:1130	arg1	terms					1105:1109	terms	1105:1109	terms of their GAG content, composition and sulfation patterns	1105:1166	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	2	27	theme	several	533:539	arg1	cell					568:571	cell growth and differentiation	568:598	cell growth and differentiation	568:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	2	27	theme	several	533:539	arg1	processes					550:558	several cellular processes	533:558	several cellular processes such as cell growth and differentiation	533:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	1	28	theme	chemical	363:370	arg1	cues					372:375	chemical cues	363:375	chemical cues that regulate major biological processes	363:416	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	2	29	theme	physiological	454:466	arg1	functions					468:476	important physiological functions	444:476	important physiological functions	444:476	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	5	30	theme	mass	1222:1225	arg1	spectrometry					1227:1238	liquid chromatography-tandem mass spectrometry	1193:1238	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	7	31	theme	method	1409:1414	arg1	effect					1381:1386	the effect	1377:1386	the effect of decellularization method on the GAG and disaccharide relative composition	1377:1463	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	0	32	theme	structural	18:27	arg1	analysis					29:36	structural analysis	18:36	structural analysis	18:36	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	1	33	theme	tissue	294:299	arg1	architecture					301:312	defining tissue architecture	285:312	defining tissue architecture	285:312	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	8	34	theme	better	1635:1640	arg1	understanding					1642:1654	a better understanding	1633:1654	a better understanding of ECM role in directing cellular responses	1633:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	3	35	theme	promising	664:672	arg1	materials					674:682	promising materials	664:682	promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds	664:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	5	36	theme	liquid	1193:1198	arg1	spectrometry					1227:1238	liquid chromatography-tandem mass spectrometry	1193:1238	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	7	37	from	effect	1381:1386	arg1	GAG					1423:1425	the GAG and disaccharide relative composition	1419:1463	GAG	1423:1425	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	7	37	from	effect	1381:1386	arg1	composition					1453:1463	the GAG and disaccharide relative composition	1419:1463	composition	1453:1463	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	8	38	link	cell-derived	1582:1593	arg1	ECM					1595:1597	cell-derived ECM	1582:1597	cell-derived ECM	1582:1597	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	8	39	theme	role	1663:1666	arg1	understanding					1642:1654	a better understanding	1633:1654	a better understanding of ECM role in directing cellular responses	1633:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	5	40	theme	cell-derived	1044:1055	arg1	ECM					1057:1059	three different cell-derived ECM	1028:1059	three different cell-derived ECM	1028:1059	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	1	41	theme	tissue	237:242	arg1	homeostasis					244:254	tissue homeostasis	237:254	tissue homeostasis	237:254	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	3	42	theme	ECM-like	765:772	arg1	composition					774:784	the native ECM-like composition	754:784	the native ECM-like composition	754:784	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	6	43	theme	different	1335:1343	arg1	ECM					1358:1360	the different cell-derived ECM	1331:1360	the different cell-derived ECM	1331:1360	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	2	44	dep	cell	568:571	arg1	differentiation					584:598	differentiation	584:598	differentiation	584:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	2	44	dep	cell	568:571	arg1	growth					573:578	growth	573:578	growth	573:578	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	0	45	link	cell-derived	63:74	arg1	matrices					90:97	cell-derived extracellular matrices	63:97	cell-derived extracellular matrices	63:97	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	5	46	link	cell-derived	1044:1055	arg1	ECM					1057:1059	three different cell-derived ECM	1028:1059	three different cell-derived ECM	1028:1059	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	8	47	theme	ECM-like	1792:1799	arg1	biomaterials					1801:1812	novel ECM-like biomaterials	1786:1812	novel ECM-like biomaterials for tissue engineering applications	1786:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	4	48	theme	GAG	970:972	arg1	composition					974:984	GAG composition	970:984	GAG composition	970:984	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	1	49	theme	mechanical	318:327	arg1	properties					329:338	mechanical properties	318:338	mechanical properties	318:338	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	50	from	role	229:232	arg1	homeostasis					244:254	tissue homeostasis	237:254	tissue homeostasis	237:254	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	50	from	role	229:232	arg1	organization					260:271	organization	260:271	organization	260:271	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	5	51	theme	GAG	1120:1122	arg1	content					1124:1130	their GAG content	1114:1130	their GAG content	1114:1130	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	8	52	theme	engineering	1825:1835	arg1	applications					1837:1848	tissue engineering applications	1818:1848	tissue engineering applications	1818:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	8	53	theme	cell-derived	1582:1593	arg1	ECM					1595:1597	cell-derived ECM	1582:1597	cell-derived ECM	1582:1597	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	6	54	theme	disaccharide	1281:1292	arg1	patterns					1304:1311	disaccharide sulfation patterns	1281:1311	disaccharide sulfation patterns	1281:1311	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	8	55	theme	GAG	1563:1565	arg1	composition					1567:1577	the GAG composition	1559:1577	the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses	1559:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	7	56	theme	relative	1444:1451	arg1	composition					1453:1463	the GAG and disaccharide relative composition	1419:1463	composition	1453:1463	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	3	57	theme	transfer	854:861	arg1	risks					863:867	pathogen transfer risks	845:867	pathogen transfer risks	845:867	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	3	58	theme	cell-derived	631:642	arg1	ECM					644:646	in vitro fabricated cell-derived ECM	611:646	in vitro fabricated cell-derived ECM	611:646	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	1	59	theme	dynamic	143:149	arg1	matrix					118:123	The extracellular matrix	100:123	The extracellular matrix (ECM)	100:129	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	59	theme	dynamic	143:149	arg1	meshwork					163:170	a highly dynamic and complex meshwork	134:170	a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs)	134:212	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	2	60	theme	signaling	503:511	arg1	pathways					513:520	signaling pathways	503:520	signaling pathways regulating several cellular processes such as cell growth and differentiation	503:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	0	61	from	analysis	29:36	arg1	matrices					90:97	cell-derived extracellular matrices	63:97	cell-derived extracellular matrices	63:97	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	6	62	theme	Distinct	1251:1258	arg1	compositions					1264:1275	Distinct GAG compositions	1251:1275	Distinct GAG compositions	1251:1275	Distinct GAG compositions and disaccharide sulfation patterns were verified for the different cell-derived ECM.					
30637588	5	63	theme	patterns	1159:1166	arg1	terms					1105:1109	terms	1105:1109	terms of their GAG content, composition and sulfation patterns	1105:1166	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	1	64	theme	complex	155:161	arg1	matrix					118:123	The extracellular matrix	100:123	The extracellular matrix (ECM)	100:129	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	64	theme	complex	155:161	arg1	meshwork					163:170	a highly dynamic and complex meshwork	134:170	a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs)	134:212	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	8	65	theme	ECM	1595:1597	arg1	composition					1567:1577	the GAG composition	1559:1577	the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses	1559:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	8	66	contain	has	1704:1706	arg1	method					1500:1505	the method	1496:1505	the method presented here	1496:1520	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	8	66	contain	has	1704:1706	arg2	potential					1712:1720	the potential	1708:1720	the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications	1708:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	3	67	theme	ECM	887:889	arg1	scaffolds					891:899	tissue-derived ECM scaffolds	872:899	tissue-derived ECM scaffolds	872:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	4	68	dep	composition	974:984	arg1	the					933:935	the	933:935	the	933:935	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	2	69	theme	cellular	541:548	arg1	cell					568:571	cell growth and differentiation	568:598	cell growth and differentiation	568:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	2	69	theme	cellular	541:548	arg1	processes					550:558	several cellular processes	533:558	several cellular processes such as cell growth and differentiation	533:598	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	3	70	link	tissue-derived	872:885	arg1	scaffolds					891:899	tissue-derived ECM scaffolds	872:899	tissue-derived ECM scaffolds	872:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	4	71	theme	cell-derived	995:1006	arg1	ECM					1008:1010	these cell-derived ECM	989:1010	these cell-derived ECM	989:1010	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	4	72	theme	ECM	1008:1010	arg1	composition					974:984	GAG composition	970:984	GAG composition	970:984	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	1	73	theme	extracellular	104:116	arg1	matrix					118:123	The extracellular matrix	100:123	The extracellular matrix (ECM)	100:129	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	73	theme	extracellular	104:116	arg1	meshwork					163:170	a highly dynamic and complex meshwork	134:170	a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs)	134:212	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	1	73	theme	extracellular	104:116	arg1	ECM					126:128	ECM	126:128	ECM	126:128	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	7	74	theme	decellularization	1391:1407	arg1	method					1409:1414	decellularization method	1391:1414	decellularization method	1391:1414	Additionally, the effect of decellularization method on the GAG and disaccharide relative composition was also assessed.					
30637588	2	75	theme	important	444:452	arg1	functions					468:476	important physiological functions	444:476	important physiological functions	444:476	GAGs are associated with important physiological functions, acting as modulators of signaling pathways regulating several cellular processes such as cell growth and differentiation.					
30637588	8	76	from	use	1760:1762	arg1	development					1771:1781	the development	1767:1781	the development of novel ECM-like biomaterials for tissue engineering applications	1767:1848	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	5	77	theme	spectrometry	1227:1238	arg1	technique					1240:1248	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	0	78	theme	glycosaminoglycans	41:58	arg1	Compositional					0:12	Compositional	0:12	Compositional	0:12	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	0	78	theme	glycosaminoglycans	41:58	arg1	analysis					29:36	structural analysis	18:36	structural analysis	18:36	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	1	79	theme	defining	285:292	arg1	architecture					301:312	defining tissue architecture	285:312	defining tissue architecture	285:312	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	0	80	theme	cell-derived	63:74	arg1	matrices					90:97	cell-derived extracellular matrices	63:97	cell-derived extracellular matrices	63:97	Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices.					
30637588	5	81	theme	sensitive	1183:1191	arg1	technique					1240:1248	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	5	82	theme	composition	1133:1143	arg1	terms					1105:1109	terms	1105:1109	terms of their GAG content, composition and sulfation patterns	1105:1166	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	8	83	theme	ECM	1659:1661	arg1	role					1663:1666	ECM role	1659:1666	ECM role in directing cellular responses	1659:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	4	84	link	cell-derived	995:1006	arg1	ECM					1008:1010	these cell-derived ECM	989:1010	these cell-derived ECM	989:1010	However, little is known about the molecular and more specifically, GAG composition of these cell-derived ECM.					
30637588	5	85	theme	chromatography-tandem	1200:1220	arg1	spectrometry					1227:1238	liquid chromatography-tandem mass spectrometry	1193:1238	a highly sensitive liquid chromatography-tandem mass spectrometry technique	1174:1248	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	3	86	theme	regenerative	688:699	arg1	medicine					701:708	regenerative medicine	688:708	regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds	688:899	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
30637588	8	87	theme	important	1737:1745	arg1	knowledge					1747:1755	important knowledge	1737:1755	important knowledge	1737:1755	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	1	88	theme	crucial	221:227	arg1	role					229:232	a crucial role	219:232	a crucial role in tissue homeostasis and organization	219:271	The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and mechanical properties, but also by providing chemical cues that regulate major biological processes.					
30637588	5	89	theme	different	1034:1042	arg1	ECM					1057:1059	three different cell-derived ECM	1028:1059	three different cell-derived ECM	1028:1059	In this study, three different cell-derived ECM were produced in vitro and characterized in terms of their GAG content, composition and sulfation patterns using a highly sensitive liquid chromatography-tandem mass spectrometry technique.					
30637588	8	90	theme	cellular	1681:1688	arg1	responses					1690:1698	cellular responses	1681:1698	cellular responses	1681:1698	In summary, the method presented here offers a novel approach to determine the GAG composition of cell-derived ECM, which we believe is critical for a better understanding of ECM role in directing cellular responses and has the potential for generating important knowledge to use in the development of novel ECM-like biomaterials for tissue engineering applications.					
30637588	3	91	link	cell-derived	631:642	arg1	ECM					644:646	in vitro fabricated cell-derived ECM	611:646	in vitro fabricated cell-derived ECM	611:646	Recently, in vitro fabricated cell-derived ECM have emerged as promising materials for regenerative medicine due to their ability of better recapitulate the native ECM-like composition and structure, without the limitations of availability and pathogen transfer risks of tissue-derived ECM scaffolds.					
31426995	6	0	theme	GPC	636:638	arg1	results					640:646	GPC results	636:646	GPC results	636:646	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	5	1	theme	105.15 mg/g	623:633	arg1	yield					614:618	a hemicelluloses yield	597:618	a hemicelluloses yield of 105.15 mg/g	597:633	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	6	2	dep	4.88 kDa	851:858	arg1	to					848:849	to	848:849	to	848:849	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	8	3	theme	extraction	1172:1181	arg1	process					1183:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	1	4	from	biomass	223:229	arg1	extraction					148:157	Microwave-assisted hydrothermal extraction	116:157	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass	116:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	5	5	theme	200 °C	517:522	arg1	temperature					502:512	A temperature	500:512	A temperature of 200 °C without holding	500:538	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	5	5	theme	200 °C	517:522	arg1	condition					576:584	the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g	557:633	the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g	557:633	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	8	6	theme	new	1197:1199	arg1	avenues					1201:1207	new avenues	1197:1207	new avenues for a sustainable tobacco-based biorefinery	1197:1251	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	4	7	theme	hemicelluloses	473:486	arg1	extraction					488:497	hemicelluloses extraction	473:497	hemicelluloses extraction	473:497	The extracted stem residue was further treated for hemicelluloses extraction.					
31426995	8	8	theme	developed	1130:1138	arg1	process					1183:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	6	9	theme	hemicelluloses	690:703	arg1	values					667:672	the Mw values	660:672	the Mw values of precipitated hemicelluloses	660:703	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	6	10	theme	un-precipitated	799:813	arg1	fraction					815:822	the un-precipitated fraction	795:822	the un-precipitated fraction	795:822	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	0	11	theme	hydrothermal	19:30	arg1	extraction					32:41	Microwave-assisted hydrothermal extraction	0:41	Microwave-assisted hydrothermal extraction of non-structural carbohydrates	0:73	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass.					
31426995	8	12	theme	sustainable	1215:1225	arg1	biorefinery					1241:1251	a sustainable tobacco-based biorefinery	1213:1251	a sustainable tobacco-based biorefinery	1213:1251	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	1	13	theme	Microwave-assisted	116:133	arg1	extraction					148:157	Microwave-assisted hydrothermal extraction	116:157	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass	116:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	0	14	theme	Microwave-assisted	0:17	arg1	extraction					32:41	Microwave-assisted hydrothermal extraction	0:41	Microwave-assisted hydrothermal extraction of non-structural carbohydrates	0:73	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass.					
31426995	3	15	theme	maximum	329:335	arg1	118.57 mg/g					371:381	118.57 mg/g	371:381	118.57 mg/g	371:381	The maximum yields for the leaf and stem were 118.57 mg/g and 120.33 mg/g biomass, respectively.					
31426995	3	15	theme	maximum	329:335	arg1	yields					337:342	The maximum yields	325:342	The maximum yields for the leaf and stem	325:364	The maximum yields for the leaf and stem were 118.57 mg/g and 120.33 mg/g biomass, respectively.					
31426995	8	16	theme	hydrothermal	1159:1170	arg1	process					1183:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	1	17	theme	hydrothermal	135:146	arg1	extraction					148:157	Microwave-assisted hydrothermal extraction	116:157	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass	116:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	6	18	theme	precipitated	677:688	arg1	hemicelluloses					690:703	precipitated hemicelluloses	677:703	precipitated hemicelluloses	677:703	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	8	19	theme	microwave-assisted	1140:1157	arg1	process					1183:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process	1126:1189	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	8	20	theme	tobacco-based	1227:1239	arg1	biorefinery					1241:1251	a sustainable tobacco-based biorefinery	1213:1251	a sustainable tobacco-based biorefinery	1213:1251	The developed microwave-assisted hydrothermal extraction process opens new avenues for a sustainable tobacco-based biorefinery.					
31426995	2	21	theme	carbohydrates	264:276	arg1	extraction					278:287	Non-structural carbohydrates extraction	249:287	Non-structural carbohydrates extraction	249:287	Non-structural carbohydrates extraction was optimized by an Optimal design.					
31426995	7	22	theme	compositional	963:975	arg1	type					977:980	heterogeneous compositional type	949:980	heterogeneous compositional type	949:980	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	7	22	theme	compositional	963:975	arg1	hemicelluloses					899:912	hemicelluloses	899:912	hemicelluloses extracted at lower temperature	899:943	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	7	23	theme	heterogeneous	949:961	arg1	type					977:980	heterogeneous compositional type	949:980	heterogeneous compositional type	949:980	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	7	23	theme	heterogeneous	949:961	arg1	hemicelluloses					899:912	hemicelluloses	899:912	hemicelluloses extracted at lower temperature	899:943	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	0	24	theme	carbohydrates	61:73	arg1	extraction					32:41	Microwave-assisted hydrothermal extraction	0:41	Microwave-assisted hydrothermal extraction of non-structural carbohydrates	0:73	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass.					
31426995	7	25	theme	lower	927:931	arg1	temperature					933:943	lower temperature	927:943	lower temperature	927:943	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	2	26	theme	Non-structural	249:262	arg1	extraction					278:287	Non-structural carbohydrates extraction	249:287	Non-structural carbohydrates extraction	249:287	Non-structural carbohydrates extraction was optimized by an Optimal design.					
31426995	5	27	theme	efficient	566:574	arg1	temperature					502:512	A temperature	500:512	A temperature of 200 °C without holding	500:538	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	5	27	theme	efficient	566:574	arg1	condition					576:584	the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g	557:633	the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g	557:633	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	0	28	theme	non-structural	46:59	arg1	carbohydrates					61:73	non-structural carbohydrates	46:73	non-structural carbohydrates	46:73	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass.					
31426995	6	29	theme	Mw	664:665	arg1	values					667:672	the Mw values	660:672	the Mw values of precipitated hemicelluloses	660:703	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	4	30	theme	extracted	426:434	arg1	residue					441:447	The extracted stem residue	422:447	The extracted stem residue	422:447	The extracted stem residue was further treated for hemicelluloses extraction.					
31426995	2	31	theme	Optimal	309:315	arg1	design					317:322	an Optimal design	306:322	an Optimal design	306:322	Non-structural carbohydrates extraction was optimized by an Optimal design.					
31426995	1	32	theme	non-structural	162:175	arg1	carbohydrates					177:189	non-structural carbohydrates	162:189	non-structural carbohydrates	162:189	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	3	33	theme	120.33 mg/g	387:397	arg1	biomass					399:405	120.33 mg/g biomass	387:405	120.33 mg/g biomass	387:405	The maximum yields for the leaf and stem were 118.57 mg/g and 120.33 mg/g biomass, respectively.					
31426995	6	34	dep	13.25 kDa	733:741	arg1	to					730:731	to	730:731	to	730:731	GPC results showed that the Mw values of precipitated hemicelluloses decreased from 143.5 kDa to 13.25 kDa with increasing temperature and holding time, while the un-precipitated fraction were ranging from 11.83 to 4.88 kDa.					
31426995	1	35	theme	carbohydrates	177:189	arg1	extraction					148:157	Microwave-assisted hydrothermal extraction	116:157	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass	116:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	4	36	theme	stem	436:439	arg1	residue					441:447	The extracted stem residue	422:447	The extracted stem residue	422:447	The extracted stem residue was further treated for hemicelluloses extraction.					
31426995	7	37	theme	xylose	1051:1056	arg1	ratio					1042:1046	the ratio	1038:1046	the ratio of xylose	1038:1056	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	7	38	theme	Monosaccharide	861:874	arg1	analysis					876:883	Monosaccharide analysis	861:883	Monosaccharide analysis	861:883	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	5	39	theme	hemicelluloses	599:612	arg1	yield					614:618	a hemicelluloses yield	597:618	a hemicelluloses yield of 105.15 mg/g	597:633	A temperature of 200 °C without holding was proved to be the most efficient condition to produce a hemicelluloses yield of 105.15 mg/g.					
31426995	1	40	theme	hemicelluloses	195:208	arg1	extraction					148:157	Microwave-assisted hydrothermal extraction	116:157	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass	116:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	0	41	theme	tobacco	99:105	arg1	biomass					107:113	tobacco biomass	99:113	tobacco biomass	99:113	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass.					
31426995	7	42	dep	72.64	1089:1093	arg1	to					1086:1087	to	1086:1087	to	1086:1087	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31426995	3	43	dep	leaf	352:355	arg1	the					348:350	the	348:350	the	348:350	The maximum yields for the leaf and stem were 118.57 mg/g and 120.33 mg/g biomass, respectively.					
31426995	1	44	theme	tobacco	215:221	arg1	biomass					223:229	tobacco biomass	215:229	tobacco biomass	215:229	Microwave-assisted hydrothermal extraction of non-structural carbohydrates and hemicelluloses from tobacco biomass was investigated.					
31426995	7	45	dep	increased	1058:1066	arg1	%					1094:1094	up to 72.64%	1083:1094	up to 72.64%	1083:1094	Monosaccharide analysis revealed that hemicelluloses extracted at lower temperature are heterogeneous compositional type, including xylan, glucuronoxylan and xylanglucan, while the ratio of xylose increased significantly (up to 72.64%) with increasing temperature.					
31771413	5	0	theme	model	749:753	arg1	system					755:760	the Caenorhabditis elegans model system	722:760	the Caenorhabditis elegans model system	722:760	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	7	1	theme	genetic	1219:1225	arg1	evidence					1227:1234	genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection	1219:1345	genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection	1219:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	5	2	used	used	717:720	arg2	we					714:715	we	714:715	we	714:715	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	4	3	theme	Hailey-Hailey	677:689	arg1	disease					691:697	Hailey-Hailey disease	677:697	Hailey-Hailey disease	677:697	PMR-1 function is evolutionary conserved from yeast to human, where mutations in the orthologous gene ATP2C1 cause Hailey-Hailey disease.					
31771413	4	4	theme	PMR-1	562:566	arg1	function					568:575	PMR-1 function	562:575	PMR-1 function	562:575	PMR-1 function is evolutionary conserved from yeast to human, where mutations in the orthologous gene ATP2C1 cause Hailey-Hailey disease.					
31771413	7	5	dep	interfered	1247:1256	arg1	pmr-1					1241:1245	pmr-1	1241:1245	pmr-1	1241:1245	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	6	6	gly	glycoproteins	944:956	arg1	glycoproteins					944:956	glycoproteins	944:956	glycoproteins	944:956	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	7	7	dep	C.	1154:1155	arg1	elegans					1157:1163	elegans	1157:1163	elegans	1157:1163	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	2	8	theme	spatial	310:316	arg1	manner					331:336	a spatial and temporal manner	308:336	a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology	308:408	Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology.					
31771413	7	9	theme	microbial	1190:1198	arg1	agents					1200:1205	microbial agents	1190:1205	microbial agents	1190:1205	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	0	10	theme	response	128:135	arg1	pathways					137:144	stress response pathways	121:144	stress response pathways	121:144	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	7	11	gly	glycoproteins	1173:1185	arg1	glycoproteins					1173:1185	C. elegans surface glycoproteins	1154:1185	C. elegans surface glycoproteins	1154:1185	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	2	12	theme	host	394:397	arg1	physiology					399:408	the host physiology	390:408	the host physiology	390:408	Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology.					
31771413	1	13	theme	different	189:197	arg1	pathways					199:206	different pathways	189:206	different pathways involved in an extreme variety of biological processes	189:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	0	14	theme	stress	121:126	arg1	pathways					137:144	stress response pathways	121:144	stress response pathways	121:144	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	5	15	theme	downstream	787:796	arg1	response					798:805	the downstream response	783:805	the downstream response elicited by the loss of pmr-1 gene	783:840	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	5	16	theme	gene	837:840	arg1	loss					823:826	the loss	819:826	the loss of pmr-1 gene	819:840	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	7	17	theme	C.	1154:1155	arg1	glycoproteins					1173:1185	C. elegans surface glycoproteins	1154:1185	C. elegans surface glycoproteins	1154:1185	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	7	18	theme	increased	1065:1073	arg1	resistance					1075:1084	increased resistance	1065:1084	increased resistance to the infection	1065:1101	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	5	19	theme	Caenorhabditis	726:739	arg1	system					755:760	the Caenorhabditis elegans model system	722:760	the Caenorhabditis elegans model system	722:760	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	4	20	theme	gene	659:662	arg1	ATP2C1					664:669	the orthologous gene ATP2C1	643:669	the orthologous gene ATP2C1	643:669	PMR-1 function is evolutionary conserved from yeast to human, where mutations in the orthologous gene ATP2C1 cause Hailey-Hailey disease.					
31771413	0	21	theme	Pmr-1	0:4	arg1	gene					6:9	Pmr-1 gene	0:9	Pmr-1 gene	0:9	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	6	22	theme	glycoproteins	944:956	arg1	structure					931:939	the oligosaccharide structure	911:939	the oligosaccharide structure of glycoproteins	911:956	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	4	23	from	mutations	630:638	arg1	ATP2C1					664:669	the orthologous gene ATP2C1	643:669	the orthologous gene ATP2C1	643:669	PMR-1 function is evolutionary conserved from yeast to human, where mutations in the orthologous gene ATP2C1 cause Hailey-Hailey disease.					
31771413	4	24	theme	orthologous	647:657	arg1	ATP2C1					664:669	the orthologous gene ATP2C1	643:669	the orthologous gene ATP2C1	643:669	PMR-1 function is evolutionary conserved from yeast to human, where mutations in the orthologous gene ATP2C1 cause Hailey-Hailey disease.					
31771413	7	25	theme	recognition	1139:1149	arg1	lack					1123:1126	lack	1123:1126	lack of regular recognition of C. elegans surface glycoproteins by microbial agents	1123:1205	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	8	26	theme	immune	1448:1453	arg1	response					1455:1462	the worm's innate immune response	1430:1462	the worm's innate immune response	1430:1462	Thus, our observations indicate pmr-1 as a candidate gene implicated in mediating the worm's innate immune response.					
31771413	2	27	theme	temporal	322:329	arg1	manner					331:336	a spatial and temporal manner	308:336	a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology	308:408	Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology.					
31771413	8	28	theme	candidate	1391:1399	arg1	gene					1401:1404	a candidate gene	1389:1404	a candidate gene implicated in mediating the worm's innate immune response	1389:1462	Thus, our observations indicate pmr-1 as a candidate gene implicated in mediating the worm's innate immune response.					
31771413	8	28	theme	candidate	1391:1399	arg1	pmr-1					1380:1384	pmr-1	1380:1384	pmr-1	1380:1384	Thus, our observations indicate pmr-1 as a candidate gene implicated in mediating the worm's innate immune response.					
31771413	7	29	theme	immune	1294:1299	arg1	response					1301:1308	a stronger innate immune response	1276:1308	a stronger innate immune response to Gram-positive bacterial infection	1276:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	8	30	theme	innate	1441:1446	arg1	response					1455:1462	the worm's innate immune response	1430:1462	the worm's innate immune response	1430:1462	Thus, our observations indicate pmr-1 as a candidate gene implicated in mediating the worm's innate immune response.					
31771413	3	31	theme	calcium	541:547	arg1	homeostasis					549:559	calcium homeostasis	541:559	calcium homeostasis	541:559	The Ca2+-ATPase, encoded by pmr-1 gene, was first identified in yeast and localized to the Golgi and it appears to be involved in calcium homeostasis.					
31771413	7	32	theme	regular	1131:1137	arg1	recognition					1139:1149	regular recognition	1131:1149	regular recognition of C. elegans surface glycoproteins by microbial agents	1131:1205	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	1	33	theme	extreme	223:229	arg1	variety					231:237	an extreme variety	220:237	an extreme variety of biological processes	220:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	1	33	theme	extreme	223:229	arg1	processes					253:261	biological processes	242:261	biological processes	242:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	0	34	theme	Caenorhabditis	37:50	arg1	elegans					52:58	Caenorhabditis elegans	37:58	Caenorhabditis elegans	37:58	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	6	35	theme	oligosaccharide	915:929	arg1	structure					931:939	the oligosaccharide structure	911:939	the oligosaccharide structure of glycoproteins	911:956	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	7	36	theme	stronger	1278:1285	arg1	response					1301:1308	a stronger innate immune response	1276:1308	a stronger innate immune response to Gram-positive bacterial infection	1276:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	2	37	theme	Calcium	264:270	arg1	levels					272:277	Calcium levels	264:277	Calcium levels	264:277	Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology.					
31771413	3	38	theme	pmr-1	439:443	arg1	gene					445:448	pmr-1 gene	439:448	pmr-1 gene	439:448	The Ca2+-ATPase, encoded by pmr-1 gene, was first identified in yeast and localized to the Golgi and it appears to be involved in calcium homeostasis.					
31771413	6	39	theme	reduced	1009:1015	arg1	susceptibility					1017:1030	reduced susceptibility	1009:1030	reduced susceptibility to bacterial infection	1009:1053	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	5	40	theme	pmr-1	831:835	arg1	gene					837:840	pmr-1 gene	831:840	pmr-1 gene	831:840	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	6	41	theme	cell	965:968	arg1	surface					970:976	the cell surface	961:976	the cell surface	961:976	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	7	42	theme	bacterial	1327:1335	arg1	infection					1337:1345	Gram-positive bacterial infection	1313:1345	Gram-positive bacterial infection	1313:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	7	43	theme	glycoproteins	1173:1185	arg1	recognition					1139:1149	regular recognition	1131:1149	regular recognition of C. elegans surface glycoproteins by microbial agents	1131:1205	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	6	44	from	defects	900:906	arg1	structure					931:939	the oligosaccharide structure	911:939	the oligosaccharide structure of glycoproteins	911:956	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	5	45	theme	elegans	741:747	arg1	system					755:760	the Caenorhabditis elegans model system	722:760	the Caenorhabditis elegans model system	722:760	In this work, we used the Caenorhabditis elegans model system to gain insight into the downstream response elicited by the loss of pmr-1 gene.					
31771413	0	46	theme	elegans	52:58	arg1	susceptibility					19:32	susceptibility	19:32	susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection	19:93	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	7	47	theme	Gram-positive	1313:1325	arg1	infection					1337:1345	Gram-positive bacterial infection	1313:1345	Gram-positive bacterial infection	1313:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	7	48	theme	interfered	1247:1256	arg1	nematodes					1258:1266	pmr-1 interfered nematodes	1241:1266	pmr-1 interfered nematodes	1241:1266	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	1	49	theme	biological	242:251	arg1	processes					253:261	biological processes	242:261	biological processes	242:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	6	50	theme	bacterial	1035:1043	arg1	infection					1045:1053	bacterial infection	1035:1053	bacterial infection	1035:1053	We found that pmr-1 knocked down animals not only showed defects in the oligosaccharide structure of glycoproteins at the cell surface but also were characterized by reduced susceptibility to bacterial infection.					
31771413	1	51	theme	processes	253:261	arg1	variety					231:237	an extreme variety	220:237	an extreme variety of biological processes	220:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	1	51	theme	processes	253:261	arg1	processes					253:261	biological processes	242:261	biological processes	242:261	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	0	52	theme	aureus	78:83	arg1	infection					85:93	Staphylococcus aureus infection	63:93	Staphylococcus aureus infection	63:93	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	7	53	theme	innate	1287:1292	arg1	response					1301:1308	a stronger innate immune response	1276:1308	a stronger innate immune response to Gram-positive bacterial infection	1276:1345	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31771413	1	54	theme	Calcium	160:166	arg1	signaling					168:176	Calcium signaling	160:176	Calcium signaling	160:176	Calcium signaling can elicit different pathways involved in an extreme variety of biological processes.					
31771413	0	55	theme	Staphylococcus	63:76	arg1	infection					85:93	Staphylococcus aureus infection	63:93	Staphylococcus aureus infection	63:93	Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways' alterations.					
31771413	7	56	theme	surface	1165:1171	arg1	glycoproteins					1173:1185	C. elegans surface glycoproteins	1154:1185	C. elegans surface glycoproteins	1154:1185	Although increased resistance to the infection might be related to lack of regular recognition of C. elegans surface glycoproteins by microbial agents, we provide genetic evidence that pmr-1 interfered nematodes mounted a stronger innate immune response to Gram-positive bacterial infection.					
31837366	0	0	theme	fecal	92:96	arg1	microflora					98:107	fecal microflora	92:107	fecal microflora	92:107	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	3	1	theme	significant	713:723	arg1	increases					725:733	significant increases	713:733	significant increases in the short chain fatty acid (SCFA) production	713:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	2	2	theme	Cordyceps	353:361	arg1	fermentation					388:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	3	3	theme	fecal	682:686	arg1	fermentation					688:699	fecal fermentation	682:699	fecal fermentation	682:699	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	5	4	theme	beneficial	1067:1076	arg1	effect					1078:1083	a beneficial effect	1065:1083	a beneficial effect	1065:1083	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	5	4	theme	beneficial	1067:1076	arg1	Proteobacteria					1036:1049	Proteobacteria	1036:1049	Proteobacteria	1036:1049	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	0	5	from	Effects	0:6	arg1	microflora					98:107	fecal microflora	92:107	fecal microflora	92:107	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	4	6	theme	EPS	870:872	arg1	fractions					874:882	the different EPS fractions	856:882	the different EPS fractions	856:882	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	5	7	theme	fecal	946:950	arg1	microflora					952:961	the fecal microflora	942:961	the fecal microflora	942:961	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	5	8	theme	relative	979:986	arg1	abundance					988:996	the relative abundance	975:996	the relative abundance of Firmicutes	975:1010	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	6	9	contain	has	1154:1156	arg1	EPS					1150:1152	the Cs-HK1 EPS	1139:1152	the Cs-HK1 EPS	1139:1152	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	6	9	contain	has	1154:1156	arg2	activity					1180:1187	significant prebiotic activity	1158:1187	significant prebiotic activity which is dependent on its molecular properties	1158:1234	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	4	10	theme	different	860:868	arg1	fractions					874:882	the different EPS fractions	856:882	the different EPS fractions	856:882	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	0	11	dep	in	116:117	arg1	vitro					119:123	vitro	119:123	vitro	119:123	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	2	12	theme	lower	513:517	arg1	MW					519:520	a lower MW	511:520	a lower MW attained by two-step ethanol precipitation	511:563	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	4	13	theme	SCFAs	829:833	arg1	rate					800:803	consumption rate	788:803	consumption rate	788:803	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	4	13	theme	SCFAs	829:833	arg1	level					820:824	production level	809:824	production level	809:824	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	1	14	theme	potential	173:181	arg1	function					193:200	the potential prebiotic function	169:200	the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus	169:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	0	15	theme	in	116:117	arg1	fermentation					125:136	in vitro fermentation	116:136	in vitro fermentation	116:136	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	1	16	dep	in	305:306	arg1	vitro					308:312	vitro	308:312	vitro	308:312	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	5	17	dep	influenced	898:907	arg1	suppressing					1016:1026	suppressing	1016:1026	suppressing that of Proteobacteria, which may be a beneficial effect for human health	1016:1100	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	5	17	dep	influenced	898:907	arg1	increasing					964:973	increasing	964:973	increasing the relative abundance of Firmicutes	964:1010	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	1	18	theme	prebiotic	183:191	arg1	function					193:200	the potential prebiotic function	169:200	the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus	169:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	2	19	with	fractions	426:434	arg1	contents					466:473	different monosaccharide contents	441:473	different monosaccharide contents	441:473	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	19	with	fractions	426:434	arg1	MW					586:587	an intermediate MW	570:587	an intermediate MW	570:587	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	19	with	fractions	426:434	arg1	MW					503:504	MW	503:504	MW	503:504	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	19	with	fractions	426:434	arg1	weight					495:500	a higher molecular weight	476:500	a higher molecular weight (MW)	476:505	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	3	20	theme	fermentation	688:699	arg1	24-48 h					671:677	24-48 h	671:677	24-48 h of fecal fermentation	671:699	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	3	21	theme	chain	748:752	arg1	SCFA					766:769	SCFA	766:769	SCFA	766:769	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	3	21	theme	chain	748:752	arg1	acid					760:763	the short chain fatty acid	738:763	the short chain fatty acid (SCFA) production	738:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	2	22	theme	EPS	618:620	arg1	ultrasound-degradation					592:613	ultrasound-degradation	592:613	ultrasound-degradation of EPS	592:620	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	23	theme	mycelial	379:386	arg1	fermentation					388:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	0	24	theme	fractions	29:37	arg1	Effects					0:6	Effects	0:6	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora	0:107	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	1	25	theme	molecular	281:289	arg1	properties					291:300	the molecular properties	277:300	the molecular properties by in vitro human fecal fermentation	277:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	26	from	relationship	261:272	arg1	fungus					246:251	a medicinal fungus	234:251	a medicinal fungus	234:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	6	27	theme	molecular	1215:1223	arg1	properties					1225:1234	its molecular properties	1211:1234	its molecular properties	1211:1234	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	1	28	theme	exopolysaccharide	205:221	arg1	function					193:200	the potential prebiotic function	169:200	the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus	169:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	28	theme	exopolysaccharide	205:221	arg1	relationship					261:272	the relationship	257:272	the relationship to the molecular properties by in vitro human fecal fermentation	257:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	0	29	theme	exopolysaccharide	11:27	arg1	fractions					29:37	exopolysaccharide fractions	11:37	exopolysaccharide fractions with different molecular weights and compositions	11:87	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	2	30	theme	ethanol	543:549	arg1	precipitation					551:563	two-step ethanol precipitation	534:563	two-step ethanol precipitation	534:563	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	6	31	theme	significant	1158:1168	arg1	activity					1180:1187	significant prebiotic activity	1158:1187	significant prebiotic activity which is dependent on its molecular properties	1158:1234	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	0	32	theme	different	44:52	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	3	33	theme	short	742:746	arg1	SCFA					766:769	SCFA	766:769	SCFA	766:769	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	3	33	theme	short	742:746	arg1	acid					760:763	the short chain fatty acid	738:763	the short chain fatty acid (SCFA) production	738:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	2	34	theme	two-step	534:541	arg1	precipitation					551:563	two-step ethanol precipitation	534:563	two-step ethanol precipitation	534:563	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	5	35	theme	Firmicutes	1001:1010	arg1	abundance					988:996	the relative abundance	975:996	the relative abundance of Firmicutes	975:1010	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	6	36	theme	prebiotic	1170:1178	arg1	activity					1180:1187	significant prebiotic activity	1158:1187	significant prebiotic activity which is dependent on its molecular properties	1158:1234	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	2	37	theme	molecular	485:493	arg1	MW					503:504	MW	503:504	MW	503:504	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	37	theme	molecular	485:493	arg1	weight					495:500	a higher molecular weight	476:500	a higher molecular weight (MW)	476:505	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	1	38	theme	in	305:306	arg1	fermentation					326:337	in vitro human fecal fermentation	305:337	in vitro human fecal fermentation	305:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	3	39	theme	acid	760:763	arg1	production					772:781	the short chain fatty acid (SCFA) production	738:781	the short chain fatty acid (SCFA) production	738:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	2	40	theme	monosaccharide	451:464	arg1	contents					466:473	different monosaccharide contents	441:473	different monosaccharide contents	441:473	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	0	41	with	fractions	29:37	arg1	compositions					76:87	compositions	76:87	compositions	76:87	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	0	41	with	fractions	29:37	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	4	42	dep	rate	800:803	arg1	The					784:786	The	784:786	The	784:786	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	2	43	theme	higher	478:483	arg1	MW					503:504	MW	503:504	MW	503:504	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	43	theme	higher	478:483	arg1	weight					495:500	a higher molecular weight	476:500	a higher molecular weight (MW)	476:505	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	3	44	used	utilized	655:662	arg2	fractions					635:643	All the EPS fractions	623:643	All the EPS fractions	623:643	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	2	45	theme	intermediate	573:584	arg1	MW					586:587	an intermediate MW	570:587	an intermediate MW	570:587	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	3	46	theme	fatty	754:758	arg1	SCFA					766:769	SCFA	766:769	SCFA	766:769	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	3	46	theme	fatty	754:758	arg1	acid					760:763	the short chain fatty acid	738:763	the short chain fatty acid (SCFA) production	738:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	5	47	theme	microflora	952:961	arg1	diversity					929:937	diversity	929:937	diversity	929:937	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	5	47	theme	microflora	952:961	arg1	composition					913:923	composition	913:923	composition	913:923	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	5	48	theme	human	1089:1093	arg1	health					1095:1100	human health	1089:1100	human health	1089:1100	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	0	49	theme	molecular	54:62	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	1	50	theme	human	314:318	arg1	fermentation					326:337	in vitro human fecal fermentation	305:337	in vitro human fecal fermentation	305:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	51	from	fungus	246:251	arg1	function					193:200	the potential prebiotic function	169:200	the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus	169:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	51	from	fungus	246:251	arg1	EPS					224:226	EPS	224:226	EPS	224:226	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	51	from	fungus	246:251	arg1	relationship					261:272	the relationship	257:272	the relationship to the molecular properties by in vitro human fecal fermentation	257:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	1	51	from	fungus	246:251	arg1	exopolysaccharide					205:221	exopolysaccharide	205:221	exopolysaccharide (EPS) from a medicinal fungus	205:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	4	52	theme	production	809:818	arg1	level					820:824	production level	809:824	production level	809:824	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	3	53	theme	EPS	631:633	arg1	fractions					635:643	All the EPS fractions	623:643	All the EPS fractions	623:643	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31837366	1	54	theme	fecal	320:324	arg1	fermentation					326:337	in vitro human fecal fermentation	305:337	in vitro human fecal fermentation	305:337	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	6	55	theme	Cs-HK1	1143:1148	arg1	EPS					1150:1152	the Cs-HK1 EPS	1139:1152	the Cs-HK1 EPS	1139:1152	Overall the results have shown that the Cs-HK1 EPS has significant prebiotic activity which is dependent on its molecular properties.					
31837366	2	56	from	fermentation	388:399	arg1	EPS					344:346	The EPS	340:346	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation	340:399	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	1	57	from	function	193:200	arg1	fungus					246:251	a medicinal fungus	234:251	a medicinal fungus	234:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	5	58	dep	composition	913:923	arg1	the					909:911	the	909:911	the	909:911	The EPS also influenced the composition and diversity of the fecal microflora, increasing the relative abundance of Firmicutes but suppressing that of Proteobacteria, which may be a beneficial effect for human health.					
31837366	4	59	theme	consumption	788:798	arg1	rate					800:803	consumption rate	788:803	consumption rate	788:803	The consumption rate and production level of SCFAs varied slightly with the different EPS fractions.					
31837366	2	60	theme	different	441:449	arg1	contents					466:473	different monosaccharide contents	441:473	different monosaccharide contents	441:473	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	2	61	theme	Cs-HK1	372:377	arg1	fermentation					388:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	1	62	theme	medicinal	236:244	arg1	fungus					246:251	a medicinal fungus	234:251	a medicinal fungus	234:251	This study was to investigate the potential prebiotic function of exopolysaccharide (EPS) from a medicinal fungus and the relationship to the molecular properties by in vitro human fecal fermentation.					
31837366	0	63	dep	Effects	0:6	arg1	fermentation					125:136	in vitro fermentation	116:136	in vitro fermentation	116:136	Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation.					
31837366	2	64	theme	sinensis	363:370	arg1	fermentation					388:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	Cordyceps sinensis Cs-HK1 mycelial fermentation	353:399	The EPS from Cordyceps sinensis Cs-HK1 mycelial fermentation was processed into three fractions with different monosaccharide contents, a higher molecular weight (MW) and a lower MW attained by two-step ethanol precipitation, and an intermediate MW by ultrasound-degradation of EPS.					
31837366	3	65	from	increases	725:733	arg1	production					772:781	the short chain fatty acid (SCFA) production	738:781	the short chain fatty acid (SCFA) production	738:781	All the EPS fractions were well utilized during 24-48 h of fecal fermentation, leading to significant increases in the short chain fatty acid (SCFA) production.					
31812750	8	0	theme	uncoated	1361:1368	arg1	TSP					1370:1372	the uncoated TSP	1357:1372	the uncoated TSP	1357:1372	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	7	1	theme	TSP	1158:1160	arg1	formulation					1162:1172	the LGCR@TSP formulation	1149:1172	the LGCR@TSP formulation composite within 3 day	1149:1195	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	6	2	theme	highest	989:995	arg1	absorbency					1003:1012	water absorbency	997:1012	water absorbency	997:1012	The results showed that the lignin-carrageenan formulation (LGCR) exhibited the highest water absorbency and elastic modulus.					
31812750	3	3	theme	alkali	614:619	arg1	method					621:626	the alkali method	610:626	the alkali method	610:626	The lignin used was extracted from olive pomace (OP) biomass using the alkali method.					
31812750	8	4	theme	100	1337:1339	arg1	%					1340:1340	%	1340:1340	%	1340:1340	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	2	5	dep	kappa-carrageenan	445:461	arg1	salt					502:505	carboxymethyl cellulose sodium salt	471:505	carboxymethyl cellulose sodium salt	471:505	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	5	dep	kappa-carrageenan	445:461	arg1	CR					464:465	CR	464:465	CR	464:465	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	1	6	theme	coating	254:260	arg1	lignin					242:247	lignin	242:247	lignin	242:247	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	1	6	theme	coating	254:260	arg1	polysaccharides					222:236	polysaccharides	222:236	polysaccharides	222:236	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	1	6	theme	coating	254:260	arg1	material					262:269	a coating material	252:269	a coating material for water-soluble triple superphosphate (TSP) granular fertilizers	252:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	8	7	theme	P	1230:1230	arg1	%					1215:1215	59.5%	1211:1215	59.5%	1211:1215	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	8	7	theme	P	1230:1230	arg1	%					1225:1225	72.5%	1221:1225	72.5%	1221:1225	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	8	7	theme	P	1230:1230	arg1	P					1230:1230	P	1230:1230	P	1230:1230	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	2	8	theme	sodium	495:500	arg1	salt					502:505	carboxymethyl cellulose sodium salt	471:505	carboxymethyl cellulose sodium salt	471:505	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	7	9	theme	Release	1035:1041	arg1	tests					1043:1047	Release tests	1035:1047	Release tests	1035:1047	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	4	10	theme	thermal	698:704	arg1	behavior					706:713	the thermal behavior	694:713	The morphological, mechanical, and surface properties as well as the thermal behavior of the coatings	629:729	The morphological, mechanical, and surface properties as well as the thermal behavior of the coatings were characterized and compared.					
31812750	6	11	theme	elastic	1018:1024	arg1	modulus					1026:1032	elastic modulus	1018:1032	elastic modulus	1018:1032	The results showed that the lignin-carrageenan formulation (LGCR) exhibited the highest water absorbency and elastic modulus.					
31812750	0	12	theme	formulation	106:116	arg1	properties					118:127	formulation properties	106:127	formulation properties	106:127	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	7	13	theme	mass	1089:1092	arg1	ratio					1094:1098	the TSP/biopolymer mass ratio	1070:1098	the TSP/biopolymer mass ratio	1070:1098	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	7	14	theme	slowest	1113:1119	arg1	release					1123:1129	the slowest P release	1109:1129	the slowest P release	1109:1129	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	1	15	theme	work	179:182	arg1	objective					161:169	The main objective	152:169	The main objective of this work	152:182	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	0	16	theme	biological	69:78	arg1	macromolecules					80:93	biological macromolecules	69:93	biological macromolecules	69:93	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	4	17	theme	surface	664:670	arg1	properties					672:681	The morphological, mechanical, and surface properties	629:681	properties	672:681	The morphological, mechanical, and surface properties as well as the thermal behavior of the coatings were characterized and compared.					
31812750	0	18	theme	New	0:2	arg1	generation					4:13	New generation	0:13	New generation of controlled release phosphorus	0:46	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	5	19	theme	scanning	807:814	arg1	SEM					837:839	SEM	837:839	SEM	837:839	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	5	19	theme	scanning	807:814	arg1	microscopy					825:834	scanning electron microscopy	807:834	scanning electron microscopy (SEM)	807:840	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	0	20	theme	controlled	18:27	arg1	phosphorus					37:46	controlled release phosphorus	18:46	controlled release phosphorus	18:46	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	1	21	theme	water-soluble	275:287	arg1	fertilizers					326:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	0	22	theme	phosphorus	132:141	arg1	release					143:149	phosphorus release	132:149	phosphorus release	132:149	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	8	23	theme	mass	1282:1285	arg1	ratios					1287:1292	the TSP/biopolymer mass ratios	1263:1292	the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively	1263:1322	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	2	24	dep	alginate	404:411	arg1	AL					441:442	AL	441:442	AL	441:442	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	24	dep	alginate	404:411	arg1	CM					508:509	CM	508:509	CM	508:509	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	24	dep	alginate	404:411	arg1	kappa-carrageenan					445:461	kappa-carrageenan	445:461	kappa-carrageenan: CR and carboxymethyl cellulose sodium salt	445:505	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	25	theme	sodium	397:402	arg1	salt					434:437	alginic acid sodium salt	414:437	alginic acid sodium salt	414:437	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	25	theme	sodium	397:402	arg1	alginate					404:411	sodium alginate	397:411	sodium alginate (alginic acid sodium salt)	397:438	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	25	theme	sodium	397:402	arg1	polysaccharides					380:394	three polysaccharides	374:394	three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM)	374:510	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	26	theme	acid	422:425	arg1	salt					434:437	alginic acid sodium salt	414:437	alginic acid sodium salt	414:437	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	26	theme	acid	422:425	arg1	alginate					404:411	sodium alginate	397:411	sodium alginate (alginic acid sodium salt)	397:438	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	1	27	theme	triple	289:294	arg1	fertilizers					326:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	8	28	theme	TSP/biopolymer	1267:1280	arg1	ratios					1287:1292	the TSP/biopolymer mass ratios	1263:1292	the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively	1263:1322	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	5	29	dep	fertilizer	875:884	arg1	materials					898:906	materials	898:906	materials	898:906	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	0	30	theme	phosphorus	37:46	arg1	generation					4:13	New generation	0:13	New generation of controlled release phosphorus	0:46	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	1	31	theme	superphosphate	296:309	arg1	fertilizers					326:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	7	32	theme	composite	1174:1182	arg1	formulation					1162:1172	the LGCR@TSP formulation	1149:1172	the LGCR@TSP formulation composite within 3 day	1149:1195	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	0	33	theme	properties	118:127	arg1	Effect					96:101	Effect	96:101	Effect of formulation properties on phosphorus release	96:149	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	6	34	theme	water	997:1001	arg1	absorbency					1003:1012	water absorbency	997:1012	water absorbency	997:1012	The results showed that the lignin-carrageenan formulation (LGCR) exhibited the highest water absorbency and elastic modulus.					
31812750	8	35	dep	released	1236:1243	arg1	compared					1325:1332	compared	1325:1332	compared to 100% P release with the uncoated TSP	1325:1372	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	0	36	theme	release	29:35	arg1	phosphorus					37:46	controlled release phosphorus	18:46	controlled release phosphorus	18:46	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	7	37	theme	ratio	1094:1098	arg1	effect					1060:1065	the effect	1056:1065	the effect of the TSP/biopolymer mass ratio	1056:1098	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	3	38	theme	olive	578:582	arg1	OP					592:593	OP	592:593	OP	592:593	The lignin used was extracted from olive pomace (OP) biomass using the alkali method.					
31812750	3	38	theme	olive	578:582	arg1	pomace					584:589	olive pomace	578:589	olive pomace (OP) biomass	578:602	The lignin used was extracted from olive pomace (OP) biomass using the alkali method.					
31812750	0	39	dep	fertilizers	48:58	arg1	Effect					96:101	Effect	96:101	Effect of formulation properties on phosphorus release	96:149	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	0	39	dep	fertilizers	48:58	arg1	based					60:64	based	60:64	fertilizers based on biological macromolecules	48:93	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	2	40	theme	cellulose	485:493	arg1	salt					502:505	carboxymethyl cellulose sodium salt	471:505	carboxymethyl cellulose sodium salt	471:505	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	1	41	theme	TSP	312:314	arg1	fertilizers					326:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	3	42	theme	pomace	584:589	arg1	biomass					596:602	olive pomace (OP) biomass	578:602	olive pomace (OP) biomass	578:602	The lignin used was extracted from olive pomace (OP) biomass using the alkali method.					
31812750	2	43	theme	sodium	427:432	arg1	salt					434:437	alginic acid sodium salt	414:437	alginic acid sodium salt	414:437	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	43	theme	sodium	427:432	arg1	alginate					404:411	sodium alginate	397:411	sodium alginate (alginic acid sodium salt)	397:438	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	7	44	theme	LGCR	1153:1156	arg1	formulation					1162:1172	the LGCR@TSP formulation	1149:1172	the LGCR@TSP formulation composite within 3 day	1149:1195	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	8	45	theme	5/1	1297:1299	arg1	ratios					1287:1292	the TSP/biopolymer mass ratios	1263:1292	the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively	1263:1322	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	8	46	theme	%	1340:1340	arg1	release					1344:1350	100% P release	1337:1350	100% P release with the uncoated TSP	1337:1372	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	1	47	theme	granular	317:324	arg1	fertilizers					326:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	water-soluble triple superphosphate (TSP) granular fertilizers	275:336	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	8	48	theme	P	1342:1342	arg1	release					1344:1350	100% P release	1337:1350	100% P release with the uncoated TSP	1337:1372	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	7	49	theme	TSP/biopolymer	1074:1087	arg1	ratio					1094:1098	the TSP/biopolymer mass ratio	1070:1098	the TSP/biopolymer mass ratio	1070:1098	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	5	50	theme	good	849:852	arg1	adhesion					854:861	good adhesion	849:861	good adhesion between the fertilizer and coating materials	849:906	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	8	51	with	3 days	1251:1256	arg1	ratios					1287:1292	the TSP/biopolymer mass ratios	1263:1292	the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively	1263:1322	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	8	52	theme	15/1	1305:1308	arg1	ratios					1287:1292	the TSP/biopolymer mass ratios	1263:1292	the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively	1263:1322	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	2	53	theme	carboxymethyl	471:483	arg1	salt					502:505	carboxymethyl cellulose sodium salt	471:505	carboxymethyl cellulose sodium salt	471:505	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	0	54	from	Effect	96:101	arg1	release					143:149	phosphorus release	132:149	phosphorus release	132:149	New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.					
31812750	4	55	theme	coatings	722:729	arg1	behavior					706:713	the thermal behavior	694:713	The morphological, mechanical, and surface properties as well as the thermal behavior of the coatings	629:729	The morphological, mechanical, and surface properties as well as the thermal behavior of the coatings were characterized and compared.					
31812750	2	56	theme	alginic	414:420	arg1	salt					434:437	alginic acid sodium salt	414:437	alginic acid sodium salt	414:437	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	2	56	theme	alginic	414:420	arg1	alginate					404:411	sodium alginate	397:411	sodium alginate (alginic acid sodium salt)	397:438	In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and carboxymethyl cellulose sodium salt: CM) and lignin (LG) were prepared.					
31812750	5	57	theme	electron	816:823	arg1	SEM					837:839	SEM	837:839	SEM	837:839	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	5	57	theme	electron	816:823	arg1	microscopy					825:834	scanning electron microscopy	807:834	scanning electron microscopy (SEM)	807:840	Their morphology and thickness revealed by scanning electron microscopy (SEM) showed good adhesion between the fertilizer and coating materials.					
31812750	7	58	theme	@	1157:1157	arg1	formulation					1162:1172	the LGCR@TSP formulation	1149:1172	the LGCR@TSP formulation composite within 3 day	1149:1195	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	7	59	theme	P	1121:1121	arg1	release					1123:1129	the slowest P release	1109:1129	the slowest P release	1109:1129	Release tests showed the effect of the TSP/biopolymer mass ratio and that the slowest P release was obtained with the LGCR@TSP formulation composite within 3 day.					
31812750	1	60	theme	main	156:159	arg1	objective					161:169	The main objective	152:169	The main objective of this work	152:182	The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers.					
31812750	8	61	with	release	1344:1350	arg1	TSP					1370:1372	the uncoated TSP	1357:1372	the uncoated TSP	1357:1372	In addition, 59.5% and 72.5% of P was released after 3 days with the TSP/biopolymer mass ratios of 5/1 and 15/1, respectively, compared to 100% P release with the uncoated TSP.					
31812750	6	62	theme	lignin-carrageenan	937:954	arg1	formulation					956:966	the lignin-carrageenan formulation	933:966	the lignin-carrageenan formulation (LGCR)	933:973	The results showed that the lignin-carrageenan formulation (LGCR) exhibited the highest water absorbency and elastic modulus.					
31812750	6	62	theme	lignin-carrageenan	937:954	arg1	LGCR					969:972	LGCR	969:972	LGCR	969:972	The results showed that the lignin-carrageenan formulation (LGCR) exhibited the highest water absorbency and elastic modulus.					
30280458	5	0	theme	MeOPN	712:716	arg1	group					718:722	a MeOPN group	710:722	a MeOPN group	710:722	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	8	1	theme	structurally-interesting	1076:1099	arg1	modification					1101:1112	this structurally-interesting modification	1071:1112	this structurally-interesting modification	1071:1112	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	1	2	theme	O-methyl	169:176	arg1	MeOPN					195:199	MeOPN	195:199	MeOPN	195:199	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	2	theme	O-methyl	169:176	arg1	phosphoramidate					178:192	O-methyl phosphoramidate	169:192	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	3	theme	Unit	86:89	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	0:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	1	4	theme	phosphoramidate	178:192	arg1	motif					202:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	4	theme	phosphoramidate	178:192	arg1	modification					232:243	a non-stoichiometric modification	211:243	a non-stoichiometric modification of capsular polysaccharides (CPS)	211:277	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	4	5	theme	absolute	545:552	arg1	stereochemistry					554:568	the absolute stereochemistry	541:568	the absolute stereochemistry at this atom	541:581	However, to date, the absolute stereochemistry at this atom has been undefined.					
30280458	0	6	theme	Absolute	103:110	arg1	Configuration					112:124	the Absolute Configuration	99:124	the Absolute Configuration of its O-Methyl Phosphoramidate Motif	99:162	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	5	7	contain	possesses	700:708	arg2	group					718:722	a MeOPN group	710:722	a MeOPN group	710:722	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	5	7	contain	possesses	700:708	arg1	these					694:698	these	694:698	these	694:698	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	5	7	contain	possesses	700:708	arg1	one					687:689	one	687:689	one	687:689	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	7	8	theme	stereochemistry	900:914	arg1	proof					891:895	the first unequivocal proof	869:895	the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS	869:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	6	9	theme	studies	748:754	arg1	course					732:737	the course	728:737	the course of these studies	728:754	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	8	10	theme	modification	1101:1112	arg1	biosynthesis					1055:1066	biosynthesis	1055:1066	biosynthesis	1055:1066	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	8	10	theme	modification	1101:1112	arg1	function					1042:1049	function	1042:1049	function	1042:1049	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	7	11	theme	CPS	947:949	arg1	jejuni					940:945	any C. jejuni CPS	933:949	any C. jejuni CPS	933:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	7	12	theme	unequivocal	879:889	arg1	proof					891:895	the first unequivocal proof	869:895	the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS	869:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	2	13	theme	food-borne	357:366	arg1	illnesses					368:376	food-borne illnesses	357:376	food-borne illnesses	357:376	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	6	14	from	stereochemistry	780:794	arg1	group					833:837	this MeOPN group	822:837	this MeOPN group	822:837	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	6	15	theme	MeOPN	827:831	arg1	group					833:837	this MeOPN group	822:837	this MeOPN group	822:837	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	3	16	theme	MeOPN	434:438	arg1	stereoisomer					509:520	a single stereoisomer	500:520	a single stereoisomer	500:520	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	3	16	theme	MeOPN	434:438	arg1	stereogenic					472:482	stereogenic	472:482	stereogenic	472:482	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	3	16	theme	MeOPN	434:438	arg1	atom					451:454	The MeOPN phosphorus atom	430:454	The MeOPN phosphorus atom in these CPS	430:467	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	5	17	theme	these	694:698	arg1	these					694:698	these	694:698	these	694:698	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	5	17	theme	these	694:698	arg1	one					687:689	one	687:689	one	687:689	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	3	18	theme	phosphorus	440:449	arg1	stereoisomer					509:520	a single stereoisomer	500:520	a single stereoisomer	500:520	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	3	18	theme	phosphorus	440:449	arg1	stereogenic					472:482	stereogenic	472:482	stereogenic	472:482	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	3	18	theme	phosphorus	440:449	arg1	atom					451:454	The MeOPN phosphorus atom	430:454	The MeOPN phosphorus atom in these CPS	430:467	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	4	19	from	atom	578:581	arg1	stereochemistry					554:568	the absolute stereochemistry	541:568	the absolute stereochemistry at this atom	541:581	However, to date, the absolute stereochemistry at this atom has been undefined.					
30280458	7	20	theme	first	873:877	arg1	proof					891:895	the first unequivocal proof	869:895	the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS	869:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	5	21	theme	units	650:654	arg1	synthesis					617:625	the synthesis	613:625	the synthesis of the three repeating units found in C. jejuni 81-176 CPS	613:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	8	22	dep	function	1042:1049	arg1	the					1038:1040	the	1038:1040	the	1038:1040	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	6	23	theme	phosphorus	803:812	arg1	atom					814:817	the phosphorus atom	799:817	the phosphorus atom	799:817	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	0	24	theme	Campylobacter	17:29	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	0	25	theme	O-Methyl	133:140	arg1	Motif					158:162	its O-Methyl Phosphoramidate Motif	129:162	its O-Methyl Phosphoramidate Motif	129:162	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	5	26	theme	jejuni	668:673	arg1	CPS					682:684	C. jejuni 81-176 CPS	665:684	C. jejuni 81-176 CPS	665:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	6	27	theme	atom	814:817	arg1	R					842:842	R	842:842	R	842:842	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	6	27	theme	atom	814:817	arg1	stereochemistry					780:794	the stereochemistry	776:794	the stereochemistry of the phosphorus atom in this MeOPN group	776:837	In the course of these studies we established that the stereochemistry of the phosphorus atom in this MeOPN group is R.					
30280458	2	28	theme	virulence	411:419	arg1	CPS					386:388	the CPS	382:388	the CPS they produce	382:401	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	2	28	theme	virulence	411:419	arg1	factors					421:427	key virulence factors	407:427	key virulence factors	407:427	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	0	29	theme	81-176	38:43	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	1	30	theme	Campylobacter	295:307	arg1	strains					316:322	all Campylobacter jejuni strains	291:322	all Campylobacter jejuni strains	291:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	31	theme	Motif	158:162	arg1	Configuration					112:124	the Absolute Configuration	99:124	the Absolute Configuration of its O-Methyl Phosphoramidate Motif	99:162	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	2	32	theme	key	407:409	arg1	CPS					386:388	the CPS	382:388	the CPS they produce	382:401	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	2	32	theme	key	407:409	arg1	factors					421:427	key virulence factors	407:427	key virulence factors	407:427	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	8	33	theme	useful	997:1002	arg1	compounds					956:964	The compounds	952:964	The compounds produced	952:973	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	8	33	theme	useful	997:1002	arg1	tools					1004:1008	useful tools	997:1008	useful tools	997:1008	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	5	34	located	found	656:660	arg2	units					650:654	the three repeating units	630:654	the three repeating units found in C. jejuni 81-176 CPS	630:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	5	34	located	found	656:660	arg1	CPS					682:684	C. jejuni 81-176 CPS	665:684	C. jejuni 81-176 CPS	665:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	1	35	theme	non-stoichiometric	213:230	arg1	motif					202:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	35	theme	non-stoichiometric	213:230	arg1	modification					232:243	a non-stoichiometric modification	211:243	a non-stoichiometric modification of capsular polysaccharides (CPS)	211:277	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	36	theme	jejuni	31:36	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	1	37	theme	jejuni	309:314	arg1	strains					316:322	all Campylobacter jejuni strains	291:322	all Campylobacter jejuni strains	291:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	38	theme	Phosphoramidate	142:156	arg1	Motif					158:162	its O-Methyl Phosphoramidate Motif	129:162	its O-Methyl Phosphoramidate Motif	129:162	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	8	39	from	tools	1004:1008	arg1	investigations					1013:1026	investigations	1013:1026	investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter	1013:1168	The compounds produced are anticipated to be useful tools in investigations targeting the function and biosynthesis of this structurally-interesting modification, which so far has only been identified in campylobacter.					
30280458	3	40	from	atom	451:454	arg1	CPS					465:467	these CPS	459:467	these CPS	459:467	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	0	41	theme	Capsular	52:59	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	1	42	theme	strains	316:322	arg1	strains					316:322	all Campylobacter jejuni strains	291:322	all Campylobacter jejuni strains	291:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	42	theme	strains	316:322	arg1	%					286:286	≈70 %	282:286	≈70 % of all Campylobacter jejuni strains	282:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	5	43	theme	repeating	640:648	arg1	units					650:654	the three repeating units	630:654	the three repeating units found in C. jejuni 81-176 CPS	630:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	1	44	from	modification	232:243	arg1	strains					316:322	all Campylobacter jejuni strains	291:322	all Campylobacter jejuni strains	291:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	44	from	modification	232:243	arg1	%					286:286	≈70 %	282:286	≈70 % of all Campylobacter jejuni strains	282:322	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	45	theme	Strain	45:50	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	5	46	theme	C.	665:666	arg1	CPS					682:684	C. jejuni 81-176 CPS	665:684	C. jejuni 81-176 CPS	665:684	We report the synthesis of the three repeating units found in C. jejuni 81-176 CPS; one of these possesses a MeOPN group.					
30280458	2	47	dep	CPS	386:388	arg1	produce					395:401	produce	395:401	produce	395:401	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	7	48	theme	C.	937:938	arg1	jejuni					940:945	any C. jejuni CPS	933:949	any C. jejuni CPS	933:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	2	49	theme	C.	339:340	arg1	jejuni					342:347	C. jejuni	339:347	C. jejuni	339:347	Infections by C. jejuni lead to food-borne illnesses and the CPS they produce are key virulence factors.					
30280458	7	50	theme	group	924:928	arg1	stereochemistry					900:914	stereochemistry	900:914	stereochemistry of this group	900:928	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	7	51	from	proof	891:895	arg1	jejuni					940:945	any C. jejuni CPS	933:949	any C. jejuni CPS	933:949	These studies represent the first unequivocal proof of stereochemistry of this group in any C. jejuni CPS.					
30280458	1	52	theme	capsular	248:255	arg1	polysaccharides					257:271	capsular polysaccharides	248:271	capsular polysaccharides (CPS)	248:277	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	52	theme	capsular	248:255	arg1	CPS					274:276	CPS	274:276	CPS	274:276	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	53	theme	Repeating	76:84	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	1	54	theme	polysaccharides	257:271	arg1	motif					202:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif	165:206	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	1	54	theme	polysaccharides	257:271	arg1	modification					232:243	a non-stoichiometric modification	211:243	a non-stoichiometric modification of capsular polysaccharides (CPS)	211:277	The O-methyl phosphoramidate (MeOPN) motif is a non-stoichiometric modification of capsular polysaccharides (CPS) in ≈70 % of all Campylobacter jejuni strains.					
30280458	0	55	theme	Polysaccharide	61:74	arg1	Unit					86:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit	13:89	Synthesis of the Campylobacter jejuni 81-176 Strain Capsular Polysaccharide Repeating Unit Reveals the Absolute Configuration of its O-Methyl Phosphoramidate Motif.					
30280458	3	56	theme	single	502:507	arg1	stereoisomer					509:520	a single stereoisomer	500:520	a single stereoisomer	500:520	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
30280458	3	56	theme	single	502:507	arg1	atom					451:454	The MeOPN phosphorus atom	430:454	The MeOPN phosphorus atom in these CPS	430:467	The MeOPN phosphorus atom in these CPS is stereogenic and is found as a single stereoisomer.					
29882469	1	0	theme	human-compatible	156:171	arg1	structures					180:189	human-compatible glycan structures	156:189	human-compatible glycan structures	156:189	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	4	1	theme	cells	608:612	arg1	defects					591:597	the growth defects	580:597	the growth defects of och1Δ cells	580:612	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	6	2	theme	promoter	805:812	arg1	region					814:819	the promoter region	801:819	the promoter region of BEM4 gene	801:832	The PB element was introduced into the promoter region of BEM4 gene and upregulated the BEM4 expression.					
29882469	1	3	theme	glycan	173:178	arg1	structures					180:189	human-compatible glycan structures	156:189	human-compatible glycan structures	156:189	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	4	4	theme	och1Δ	602:606	arg1	cells					608:612	och1Δ cells	602:612	och1Δ cells	602:612	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	0	5	theme	yeast	100:104	arg1	cells					106:110	och1-disrupted yeast cells	85:110	och1-disrupted yeast cells	85:110	PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.					
29882469	4	6	theme	in	617:618	arg1	mutagenesis					625:635	in vivo mutagenesis	617:635	in vivo mutagenesis using piggyBac (PB)-based transposon	617:672	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	0	7	theme	och1-disrupted	85:98	arg1	cells					106:110	och1-disrupted yeast cells	85:110	och1-disrupted yeast cells	85:110	PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.					
29882469	4	8	theme	-based	656:661	arg1	transposon					663:672	piggyBac (PB)-based transposon	643:672	piggyBac (PB)-based transposon	643:672	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	1	9	gly	glycoproteins	254:266	arg1	glycoproteins					254:266	therapeutic glycoproteins	242:266	therapeutic glycoproteins	242:266	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	3	10	theme	gene	467:470	arg1	disruption					472:481	the gene disruption	463:481	the gene disruption	463:481	However, the gene disruption causes cell wall defects leading to growth defects.					
29882469	2	11	theme	structures	442:451	arg1	elimination					403:413	the elimination	399:413	the elimination of yeast-specific N-glycan structures	399:451	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	3	12	theme	cell	490:493	arg1	defects					500:506	cell wall defects	490:506	cell wall defects leading to growth defects	490:532	However, the gene disruption causes cell wall defects leading to growth defects.					
29882469	5	13	theme	parental	744:751	arg1	cells					759:763	parental och1Δ cells	744:763	parental och1Δ cells	744:763	We isolated a mutant strain, named 121, which could grow faster than parental och1Δ cells.					
29882469	2	14	theme	mannan-type	350:360	arg1	formation					371:379	mannan-type N-glycan formation	350:379	mannan-type N-glycan formation	350:379	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	2	15	theme	yeast-specific	418:431	arg1	structures					442:451	yeast-specific N-glycan structures	418:451	yeast-specific N-glycan structures	418:451	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	0	16	theme	PiggyBac-based	0:13	arg1	screening					15:23	PiggyBac-based screening	0:23	PiggyBac-based screening	0:23	PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.					
29882469	4	17	theme	growth	584:589	arg1	defects					591:597	the growth defects	580:597	the growth defects of och1Δ cells	580:612	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	6	18	theme	PB	770:771	arg1	element					773:779	The PB element	766:779	The PB element	766:779	The PB element was introduced into the promoter region of BEM4 gene and upregulated the BEM4 expression.					
29882469	9	19	theme	glycoengineered	1128:1142	arg1	yeast					1144:1148	glycoengineered yeast	1128:1148	glycoengineered yeast	1128:1148	Our results indicate that BEM4 would be useful to produce therapeutic proteins in glycoengineered yeast without the growth defects.					
29882469	7	20	theme	och1Δ	923:927	arg1	cells					929:933	och1Δ cells	923:933	och1Δ cells	923:933	Overexpression of BEM4 suppressed growth defects in och1Δ cells.					
29882469	9	21	theme	growth	1162:1167	arg1	defects					1169:1175	the growth defects	1158:1175	the growth defects	1158:1175	Our results indicate that BEM4 would be useful to produce therapeutic proteins in glycoengineered yeast without the growth defects.					
29882469	4	22	dep	in	617:618	arg1	vivo					620:623	vivo	620:623	vivo	620:623	Here, we tried to identify factors to rescue the growth defects of och1Δ cells by in vivo mutagenesis using piggyBac (PB)-based transposon.					
29882469	7	23	from	defects	912:918	arg1	cells					929:933	och1Δ cells	923:933	och1Δ cells	923:933	Overexpression of BEM4 suppressed growth defects in och1Δ cells.					
29882469	1	24	theme	host	220:223	arg1	cells					225:229	host cells	220:229	host cells	220:229	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	0	25	from	defects	74:80	arg1	cells					106:110	och1-disrupted yeast cells	85:110	och1-disrupted yeast cells	85:110	PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.					
29882469	3	26	theme	wall	495:498	arg1	defects					500:506	cell wall defects	490:506	cell wall defects leading to growth defects	490:532	However, the gene disruption causes cell wall defects leading to growth defects.					
29882469	8	27	dep	phenotypes	950:959	arg1	grow					945:948	grow	945:948	grow	945:948	The slow grow phenotypes were partially rescued by expression of Rho1p, whose function is regulated by Bem4p.					
29882469	6	28	theme	BEM4	854:857	arg1	expression					859:868	the BEM4 expression	850:868	the BEM4 expression	850:868	The PB element was introduced into the promoter region of BEM4 gene and upregulated the BEM4 expression.					
29882469	9	29	theme	therapeutic	1104:1114	arg1	proteins					1116:1123	therapeutic proteins	1104:1123	therapeutic proteins	1104:1123	Our results indicate that BEM4 would be useful to produce therapeutic proteins in glycoengineered yeast without the growth defects.					
29882469	1	30	theme	Glycoengineered	113:127	arg1	cells					135:139	Glycoengineered yeast cells	113:139	Glycoengineered yeast cells	113:139	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	5	31	theme	mutant	689:694	arg1	strain					696:701	a mutant strain	687:701	a mutant strain	687:701	We isolated a mutant strain, named 121, which could grow faster than parental och1Δ cells.					
29882469	6	32	theme	gene	829:832	arg1	region					814:819	the promoter region	801:819	the promoter region of BEM4 gene	801:832	The PB element was introduced into the promoter region of BEM4 gene and upregulated the BEM4 expression.					
29882469	1	33	theme	yeast	129:133	arg1	cells					135:139	Glycoengineered yeast cells	113:139	Glycoengineered yeast cells	113:139	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	7	34	theme	growth	905:910	arg1	defects					912:918	growth defects	905:918	growth defects in och1Δ cells	905:933	Overexpression of BEM4 suppressed growth defects in och1Δ cells.					
29882469	8	35	theme	Rho1p	1001:1005	arg1	expression					987:996	expression	987:996	expression	987:996	The slow grow phenotypes were partially rescued by expression of Rho1p, whose function is regulated by Bem4p.					
29882469	6	36	theme	BEM4	824:827	arg1	gene					829:832	BEM4 gene	824:832	BEM4 gene	824:832	The PB element was introduced into the promoter region of BEM4 gene and upregulated the BEM4 expression.					
29882469	2	37	theme	gene	288:291	arg1	Disruption					269:278	Disruption	269:278	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation,	269:380	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	3	38	theme	growth	519:524	arg1	defects					526:532	growth defects	519:532	growth defects	519:532	However, the gene disruption causes cell wall defects leading to growth defects.					
29882469	2	39	theme	OCH1	283:286	arg1	α-1,6-mannosyltransferase					311:335	an α-1,6-mannosyltransferase	308:335	an α-1,6-mannosyltransferase required for mannan-type N-glycan formation	308:379	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	2	39	theme	OCH1	283:286	arg1	gene					288:291	OCH1 gene	283:291	OCH1 gene	283:291	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	8	40	theme	slow	940:943	arg1	phenotypes					950:959	The slow grow phenotypes	936:959	The slow grow phenotypes	936:959	The slow grow phenotypes were partially rescued by expression of Rho1p, whose function is regulated by Bem4p.					
29882469	2	41	theme	N-glycan	362:369	arg1	formation					371:379	mannan-type N-glycan formation	350:379	mannan-type N-glycan formation	350:379	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	7	42	theme	BEM4	889:892	arg1	Overexpression					871:884	Overexpression	871:884	Overexpression of BEM4	871:892	Overexpression of BEM4 suppressed growth defects in och1Δ cells.					
29882469	1	43	theme	therapeutic	242:252	arg1	glycoproteins					254:266	therapeutic glycoproteins	242:266	therapeutic glycoproteins	242:266	Glycoengineered yeast cells, which express human-compatible glycan structures, are particularly attractive host cells to produce therapeutic glycoproteins.					
29882469	0	44	theme	growth	67:72	arg1	defects					74:80	growth defects	67:80	growth defects in och1-disrupted yeast cells	67:110	PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.					
29882469	2	45	theme	N-glycan	433:440	arg1	structures					442:451	yeast-specific N-glycan structures	418:451	yeast-specific N-glycan structures	418:451	Disruption of OCH1 gene, which encodes an α-1,6-mannosyltransferase required for mannan-type N-glycan formation, is essential for the elimination of yeast-specific N-glycan structures.					
29882469	5	46	theme	och1Δ	753:757	arg1	cells					759:763	parental och1Δ cells	744:763	parental och1Δ cells	744:763	We isolated a mutant strain, named 121, which could grow faster than parental och1Δ cells.					
31227364	9	0	theme	important	1469:1477	arg1	mechanism					1479:1487	an important mechanism	1466:1487	an important mechanism for the function of dietary polyphenols	1466:1527	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	9	0	theme	important	1469:1477	arg1	disturbance					1421:1431	disturbance	1421:1431	disturbance of mucin-type O-glycosylation	1421:1461	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	1	1	theme	colonic	194:200	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	1	theme	colonic	194:200	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	1	theme	colonic	194:200	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	4	2	theme	enzyme	698:703	arg1	assay					705:709	HPLC-based enzyme assay	687:709	HPLC-based enzyme assay	687:709	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	0	3	theme	polyphenols	102:112	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases	0:62	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	0	3	theme	polyphenols	102:112	arg1	mechanism					81:89	an underlying mechanism	67:89	an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis	67:148	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	6	4	theme	site-directed	945:957	arg1	mutagenesis					959:969	site-directed mutagenesis	945:969	site-directed mutagenesis	945:969	Computational simulations combined with site-directed mutagenesis further support the inhibitors' mode of action.					
31227364	2	5	theme	multiple	446:453	arg1	processes					466:474	multiple biological processes	446:474	multiple biological processes	446:474	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	4	6	theme	HPLC-based	687:696	arg1	assay					705:709	HPLC-based enzyme assay	687:709	HPLC-based enzyme assay	687:709	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	9	7	theme	mucin-type	1436:1445	arg1	O-glycosylation					1447:1461	mucin-type O-glycosylation	1436:1461	mucin-type O-glycosylation	1436:1461	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	9	8	theme	cell	1322:1325	arg1	O-glycosylation					1327:1341	cell O-glycosylation	1322:1341	cell O-glycosylation	1322:1341	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	1	9	theme	metabolite	202:211	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	9	theme	metabolite	202:211	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	9	theme	metabolite	202:211	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	0	10	theme	colorectal	125:134	arg1	tumorigenesis					136:148	colorectal tumorigenesis	125:148	colorectal tumorigenesis	125:148	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	9	11	theme	O-glycosylation	1447:1461	arg1	mechanism					1479:1487	an important mechanism	1466:1487	an important mechanism for the function of dietary polyphenols	1466:1527	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	9	11	theme	O-glycosylation	1447:1461	arg1	disturbance					1421:1431	disturbance	1421:1431	disturbance of mucin-type O-glycosylation	1421:1461	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	6	12	theme	Computational	905:917	arg1	simulations					919:929	Computational simulations	905:929	Computational simulations combined with site-directed mutagenesis	905:969	Computational simulations combined with site-directed mutagenesis further support the inhibitors' mode of action.					
31227364	2	13	theme	biological	455:464	arg1	processes					466:474	multiple biological processes	446:474	multiple biological processes	446:474	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	3	14	theme	tumor	598:602	arg1	behavior					604:611	tumor behavior	598:611	tumor behavior of colorectal cancer cells (CRC)	598:644	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	5	15	with	manner	873:878	arg1	affinity					895:902	nanomolar affinity	885:902	nanomolar affinity	885:902	In particular, UroD inhibits ppGalNAc-T2 through a peptide/protein-competitive manner with nanomolar affinity.					
31227364	2	16	theme	Mucin-type	329:338	arg1	O-glycosylation					340:354	Mucin-type O-glycosylation	329:354	Mucin-type O-glycosylation	329:354	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	5	17	theme	peptide/protein-competitive	845:871	arg1	manner					873:878	a peptide/protein-competitive manner	843:878	a peptide/protein-competitive manner with nanomolar affinity	843:902	In particular, UroD inhibits ppGalNAc-T2 through a peptide/protein-competitive manner with nanomolar affinity.					
31227364	6	18	theme	action	1011:1016	arg1	mode					1003:1006	the inhibitors' mode	987:1006	the inhibitors' mode of action	987:1016	Computational simulations combined with site-directed mutagenesis further support the inhibitors' mode of action.					
31227364	1	19	theme	functional	228:237	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	19	theme	functional	228:237	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	19	theme	functional	228:237	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	9	20	theme	specific	1236:1243	arg1	polyphenols					1258:1268	specific tannin-based polyphenols	1236:1268	specific tannin-based polyphenols	1236:1268	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	1	21	theme	dietary	239:245	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	21	theme	dietary	239:245	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	21	theme	dietary	239:245	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	0	22	theme	N-acetyl-α-galactosaminyltransferases	26:62	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases	0:62	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	0	22	theme	N-acetyl-α-galactosaminyltransferases	26:62	arg1	mechanism					81:89	an underlying mechanism	67:89	an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis	67:148	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	8	23	theme	cells	1208:1212	arg1	invasion					1192:1199	invasion	1192:1199	invasion	1192:1199	Transwell experiments prove that UroD inhibits migration and invasion of CRC cells.					
31227364	8	23	theme	cells	1208:1212	arg1	migration					1178:1186	migration	1178:1186	migration	1178:1186	Transwell experiments prove that UroD inhibits migration and invasion of CRC cells.					
31227364	7	24	theme	metabolic	1049:1057	arg1	labelling					1059:1067	metabolic labelling	1049:1067	metabolic labelling	1049:1067	Moreover, lectin analysis and metabolic labelling reveal that UroD can reduce cell O-glycans but not N-glycans.					
31227364	2	25	theme	polypeptide	370:380	arg1	ppGalNAc-Ts					421:431	ppGalNAc-Ts	421:431	ppGalNAc-Ts	421:431	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	2	25	theme	polypeptide	370:380	arg1	N-acetyl-α-galactosaminyltransferases					382:418	polypeptide N-acetyl-α-galactosaminyltransferases	370:418	polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts)	370:432	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	3	26	theme	specific	557:564	arg1	polyphenols					579:589	specific tannin-based polyphenols	557:589	specific tannin-based polyphenols	557:589	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	0	27	theme	polypeptide	14:24	arg1	N-acetyl-α-galactosaminyltransferases					26:62	polypeptide N-acetyl-α-galactosaminyltransferases	14:62	polypeptide N-acetyl-α-galactosaminyltransferases	14:62	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	8	28	theme	CRC	1204:1206	arg1	cells					1208:1212	CRC cells	1204:1212	CRC cells	1204:1212	Transwell experiments prove that UroD inhibits migration and invasion of CRC cells.					
31227364	1	29	theme	Ellagitannin-derived	151:170	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	29	theme	Ellagitannin-derived	151:170	arg1	EA					186:187	EA	186:187	EA	186:187	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	29	theme	Ellagitannin-derived	151:170	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	29	theme	Ellagitannin-derived	151:170	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	3	30	theme	tannin-based	566:577	arg1	polyphenols					579:589	specific tannin-based polyphenols	557:589	specific tannin-based polyphenols	557:589	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	4	31	theme	gallic	747:752	arg1	GA					760:761	GA	760:761	GA	760:761	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	4	31	theme	gallic	747:752	arg1	acid					754:757	gallic acid	747:757	gallic acid (GA)	747:762	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	1	32	theme	cancer	263:268	arg1	prevention					270:279	cancer prevention	263:279	cancer prevention	263:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	9	33	theme	tannin-based	1245:1256	arg1	polyphenols					1258:1268	specific tannin-based polyphenols	1236:1268	specific tannin-based polyphenols	1236:1268	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	2	34	theme	cancer	507:512	arg1	progression					514:524	cancer progression	507:524	cancer progression	507:524	Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression.					
31227364	5	35	theme	nanomolar	885:893	arg1	affinity					895:902	nanomolar affinity	885:902	nanomolar affinity	885:902	In particular, UroD inhibits ppGalNAc-T2 through a peptide/protein-competitive manner with nanomolar affinity.					
31227364	9	36	theme	dietary	1509:1515	arg1	polyphenols					1517:1527	dietary polyphenols	1509:1527	dietary polyphenols	1509:1527	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	1	37	theme	ellagic	172:178	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	37	theme	ellagic	172:178	arg1	EA					186:187	EA	186:187	EA	186:187	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	37	theme	ellagic	172:178	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	37	theme	ellagic	172:178	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	0	38	theme	underlying	70:79	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases	0:62	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	0	38	theme	underlying	70:79	arg1	mechanism					81:89	an underlying mechanism	67:89	an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis	67:148	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	9	39	theme	polyphenols	1517:1527	arg1	function					1497:1504	the function	1493:1504	the function of dietary polyphenols	1493:1527	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	9	40	theme	CRC	1394:1396	arg1	cells					1398:1402	CRC cells	1394:1402	CRC cells	1394:1402	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	3	41	theme	colorectal	616:625	arg1	CRC					641:643	CRC	641:643	CRC	641:643	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	3	41	theme	colorectal	616:625	arg1	cells					634:638	colorectal cancer cells	616:638	colorectal cancer cells (CRC)	616:644	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	7	42	theme	lectin	1029:1034	arg1	analysis					1036:1043	lectin analysis	1029:1043	lectin analysis	1029:1043	Moreover, lectin analysis and metabolic labelling reveal that UroD can reduce cell O-glycans but not N-glycans.					
31227364	8	43	theme	Transwell	1131:1139	arg1	experiments					1141:1151	Transwell experiments	1131:1151	Transwell experiments	1131:1151	Transwell experiments prove that UroD inhibits migration and invasion of CRC cells.					
31227364	9	44	theme	cells	1398:1402	arg1	invasion					1382:1389	invasion	1382:1389	invasion	1382:1389	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	9	44	theme	cells	1398:1402	arg1	migration					1368:1376	migration	1368:1376	migration	1368:1376	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	4	45	dep	find	715:718	arg1	inhibit					773:779	inhibit	773:779	find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts	715:791	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	0	46	theme	dietary	94:100	arg1	polyphenols					102:112	dietary polyphenols	94:112	dietary polyphenols preventing colorectal tumorigenesis	94:148	Inhibition of polypeptide N-acetyl-α-galactosaminyltransferases is an underlying mechanism of dietary polyphenols preventing colorectal tumorigenesis.					
31227364	9	47	theme	ppGalNAc-Ts	1291:1301	arg1	activity					1303:1310	ppGalNAc-Ts activity	1291:1310	ppGalNAc-Ts activity	1291:1310	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	3	48	theme	cancer	627:632	arg1	CRC					641:643	CRC	641:643	CRC	641:643	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	3	48	theme	cancer	627:632	arg1	cells					634:638	colorectal cancer cells	616:638	colorectal cancer cells (CRC)	616:644	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	9	49	dep	migration	1368:1376	arg1	the					1364:1366	the	1364:1366	the	1364:1366	Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols.					
31227364	1	50	link	Ellagitannin-derived	151:170	arg1	urolithins					213:222	colonic metabolite urolithins	194:222	colonic metabolite urolithins	194:222	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	50	link	Ellagitannin-derived	151:170	arg1	EA					186:187	EA	186:187	EA	186:187	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	50	link	Ellagitannin-derived	151:170	arg1	acid					180:183	Ellagitannin-derived ellagic acid	151:183	Ellagitannin-derived ellagic acid (EA)	151:188	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	1	50	link	Ellagitannin-derived	151:170	arg1	ingredients					247:257	functional dietary ingredients	228:257	functional dietary ingredients for cancer prevention	228:279	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
31227364	4	51	theme	urolithin	720:728	arg1	UroD					733:736	UroD	733:736	UroD	733:736	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	4	51	theme	urolithin	720:728	arg1	D					730:730	urolithin D	720:730	urolithin D (UroD)	720:737	Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts.					
31227364	7	52	theme	cell	1097:1100	arg1	O-glycans					1102:1110	cell O-glycans	1097:1110	cell O-glycans but not N-glycans	1097:1128	Moreover, lectin analysis and metabolic labelling reveal that UroD can reduce cell O-glycans but not N-glycans.					
31227364	3	53	theme	cells	634:638	arg1	behavior					604:611	tumor behavior	598:611	tumor behavior of colorectal cancer cells (CRC)	598:644	Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation.					
31227364	1	54	theme	underlying	290:299	arg1	mechanism					301:309	the underlying mechanism	286:309	the underlying mechanism	286:309	Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation.					
29412175	6	0	theme	α-d-glucosidase	934:948	arg1	activity					961:968	α-d-glucosidase inhibitory activity	934:968	α-d-glucosidase inhibitory activity	934:968	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	5	1	from	106.6KDa	880:887	arg1	PR-1					826:829	PR-1	826:829	PR-1 (from 6.2×103 to 7.4KDa)	826:854	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	5	1	from	106.6KDa	880:887	arg1	fractions					815:823	two fractions	811:823	two fractions	811:823	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	5	1	from	106.6KDa	880:887	arg1	PR-2					860:863	PR-2	860:863	PR-2 (from 559.8 to 106.6KDa)	860:888	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	8	2	theme	%	1505:1505	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	4	3	dep	power	551:555	arg1	18min					580:584	18min	580:584	18min	580:584	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	575W					558:561	575W	558:561	575W	558:561	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	dose					636:639	enzyme dose	629:639	enzyme dose	629:639	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	ratio					606:610	liquid-to-material ratio	587:610	liquid-to-material ratio	587:610	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	13.5:1mL/g					613:622	13.5:1mL/g	613:622	13.5:1mL/g	613:622	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	6.5g/mL					642:648	6.5g/mL	642:648	6.5g/mL	642:648	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	3	dep	power	551:555	arg1	time					574:577	microwave time	564:577	microwave time	564:577	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	3	4	theme	Plackett-Burman	399:413	arg1	design					420:425	Plackett-Burman (PB) design	399:425	Plackett-Burman (PB) design	399:425	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	8	5	with	rhamnose	1315:1322	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	4	6	theme	Specific	520:527	arg1	conditions					529:538	Specific conditions	520:538	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL)	520:649	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	6	theme	Specific	520:527	arg1	power					551:555	microwave power	541:555	microwave power	541:555	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	7	theme	microwave	541:549	arg1	conditions					529:538	Specific conditions	520:538	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL)	520:649	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	4	7	theme	microwave	541:549	arg1	power					551:555	microwave power	541:555	microwave power	541:555	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	8	8	theme	%	1478:1478	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	9	theme	%	1498:1498	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	10	theme	%	1491:1491	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	11	theme	%	1471:1471	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	3	12	theme	central	431:437	arg1	design					449:454	central composite design	431:454	central composite design	431:454	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	6	13	theme	antioxidant	909:919	arg1	activity					921:928	the antioxidant activity	905:928	the antioxidant activity	905:928	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	5	14	dep	7.4KDa	848:853	arg1	to					845:846	to	845:846	to	845:846	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	7	15	theme	1-phenyl-3-methyl-5-pyrazolone	1196:1225	arg1	method					1252:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
29412175	5	16	theme	cellulose	784:792	arg1	column					794:799	a DEAE-52 cellulose column	774:799	a DEAE-52 cellulose column	774:799	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	6	17	theme	α-d-glucosidase	1070:1084	arg1	activity					1097:1104	α-d-glucosidase inhibitory activity	1070:1104	α-d-glucosidase inhibitory activity	1070:1104	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	2	18	from	polysaccharides	270:284	arg1	roxburghii					294:303	Rosa roxburghii	289:303	Rosa roxburghii	289:303	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	4	19	theme	microwave	564:572	arg1	time					574:577	microwave time	564:577	microwave time	564:577	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	3	20	dep	Rosa	471:474	arg1	roxburghii					476:485	roxburghii	476:485	roxburghii	476:485	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	2	21	theme	surface	334:340	arg1	methodology					342:352	response surface methodology	325:352	response surface methodology	325:352	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	5	22	from	7.4KDa	848:853	arg1	PR-1					826:829	PR-1	826:829	PR-1 (from 6.2×103 to 7.4KDa)	826:854	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	5	22	from	7.4KDa	848:853	arg1	fractions					815:823	two fractions	811:823	two fractions	811:823	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	5	22	from	7.4KDa	848:853	arg1	PR-2					860:863	PR-2	860:863	PR-2 (from 559.8 to 106.6KDa)	860:888	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	7	23	theme	monosaccharide	1130:1143	arg1	composition					1145:1155	the monosaccharide composition	1126:1155	the monosaccharide composition of PR-1	1126:1163	Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
29412175	8	24	with	acid	1363:1366	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	3	25	theme	extraction	502:511	arg1	yield					513:517	the Rosa roxburghii polysaccharide extraction yield	467:517	the Rosa roxburghii polysaccharide extraction yield	467:517	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	7	26	theme	precolumn	1227:1235	arg1	method					1252:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
29412175	0	27	theme	microwave-assisted	20:37	arg1	extraction					49:58	the microwave-assisted enzymatic extraction	16:58	the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt	16:83	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	1	28	theme	response	108:115	arg1	methodology					125:135	response surface methodology	108:135	response surface methodology	108:135	polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity.					
29412175	6	29	theme	stronger	1036:1043	arg1	activity					1057:1064	stronger antioxidant activity	1036:1064	stronger antioxidant activity	1036:1064	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	8	30	with	acid	1382:1385	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	3	31	theme	composite	439:447	arg1	design					449:454	central composite design	431:454	central composite design	431:454	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	8	32	theme	%	1512:1512	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	1	33	theme	surface	117:123	arg1	methodology					125:135	response surface methodology	108:135	response surface methodology	108:135	polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity.					
29412175	7	34	theme	PR-1	1160:1163	arg1	composition					1145:1155	the monosaccharide composition	1126:1155	the monosaccharide composition of PR-1	1126:1163	Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
29412175	4	35	theme	%	696:696	arg1	yield					677:681	an experimental yield	661:681	an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%	661:753	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	8	36	theme	molar	1434:1438	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	6	37	theme	fractions	996:1004	arg1	activity					961:968	α-d-glucosidase inhibitory activity	934:968	α-d-glucosidase inhibitory activity	934:968	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	6	37	theme	fractions	996:1004	arg1	activity					921:928	the antioxidant activity	905:928	the antioxidant activity	905:928	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	8	38	theme	%	1458:1458	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	39	with	galactose	1397:1405	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	40	theme	%	1522:1522	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	0	41	theme	extraction	49:58	arg1	Optimization					0:11	Optimization	0:11	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.	0:84	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	4	42	theme	enzyme	629:634	arg1	dose					636:639	enzyme dose	629:639	enzyme dose	629:639	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	8	43	with	hydrochloride	1337:1349	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	6	44	theme	polysaccharide	981:994	arg1	fractions					996:1004	the two polysaccharide fractions	973:1004	the two polysaccharide fractions	973:1004	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	0	45	theme	enzymatic	39:47	arg1	extraction					49:58	the microwave-assisted enzymatic extraction	16:58	the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt	16:83	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	3	46	theme	polysaccharide	487:500	arg1	yield					513:517	the Rosa roxburghii polysaccharide extraction yield	467:517	the Rosa roxburghii polysaccharide extraction yield	467:517	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	2	47	theme	extraction	201:210	arg1	assay					212:216	An extraction assay	198:216	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii	198:303	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	2	48	theme	Rosa	289:292	arg1	roxburghii					294:303	Rosa roxburghii	289:303	Rosa roxburghii	289:303	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	8	49	with	arabinose	1408:1416	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	6	50	theme	inhibitory	1086:1095	arg1	activity					1097:1104	α-d-glucosidase inhibitory activity	1070:1104	α-d-glucosidase inhibitory activity	1070:1104	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	0	51	theme	Rosa	63:66	arg1	Tratt					79:83	Rosa roxburghii Tratt	63:83	Rosa roxburghii Tratt	63:83	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	4	52	theme	liquid-to-material	587:604	arg1	ratio					606:610	liquid-to-material ratio	587:610	liquid-to-material ratio	587:610	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	0	53	theme	roxburghii	68:77	arg1	Tratt					79:83	Rosa roxburghii Tratt	63:83	Rosa roxburghii Tratt	63:83	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	8	54	contain	contained	1288:1296	arg2	ribose					1307:1312	ribose	1307:1312	ribose	1307:1312	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg1	PR-1					1283:1286	PR-1	1283:1286	PR-1	1283:1286	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	hydrochloride					1337:1349	glucosamine hydrochloride	1325:1349	glucosamine hydrochloride	1325:1349	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	acid					1382:1385	galacturonic acid	1369:1385	galacturonic acid	1369:1385	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	acid					1363:1366	glucuronic acid	1352:1366	glucuronic acid	1352:1366	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	arabinose					1408:1416	arabinose	1408:1416	arabinose	1408:1416	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	glucose					1388:1394	glucose	1388:1394	glucose	1388:1394	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	fucose					1422:1427	fucose	1422:1427	fucose	1422:1427	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	galactose					1397:1405	galactose	1397:1405	galactose	1397:1405	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	rhamnose					1315:1322	rhamnose	1315:1322	rhamnose	1315:1322	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	54	contain	contained	1288:1296	arg2	mannose					1298:1304	mannose	1298:1304	mannose	1298:1304	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	8	55	theme	galacturonic	1369:1380	arg1	acid					1382:1385	galacturonic acid	1369:1385	galacturonic acid	1369:1385	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	3	56	theme	Rosa	471:474	arg1	yield					513:517	the Rosa roxburghii polysaccharide extraction yield	467:517	the Rosa roxburghii polysaccharide extraction yield	467:517	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	8	57	theme	%	1465:1465	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	5	58	dep	106.6KDa	880:887	arg1	to					877:878	to	877:878	to	877:878	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	4	59	theme	%	753:753	arg1	value					739:743	the predicted value	725:743	the predicted value of 35.75%	725:753	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	7	60	theme	derivatization	1237:1250	arg1	method					1252:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	1194:1257	Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
29412175	3	61	theme	process	359:365	arg1	parameters					367:376	The process parameters	355:376	The process parameters	355:376	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	8	62	theme	%	1484:1484	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	2	63	theme	response	325:332	arg1	methodology					342:352	response surface methodology	325:352	response surface methodology	325:352	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	8	64	theme	glucosamine	1325:1335	arg1	hydrochloride					1337:1349	glucosamine hydrochloride	1325:1349	glucosamine hydrochloride	1325:1349	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	1	65	theme	antioxidant	145:155	arg1	activity					188:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity.					
29412175	4	66	theme	experimental	664:675	arg1	yield					677:681	an experimental yield	661:681	an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%	661:753	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	2	67	theme	enzymatic	246:254	arg1	treatment					256:264	microwave-assisted enzymatic treatment	227:264	microwave-assisted enzymatic treatment	227:264	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	8	68	with	ribose	1307:1312	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	4	69	theme	predicted	729:737	arg1	value					739:743	the predicted value	725:743	the predicted value of 35.75%	725:753	Specific conditions (microwave power, 575W; microwave time, 18min; liquid-to-material ratio, 13.5:1mL/g; and enzyme dose, 6.5g/mL) generated an experimental yield of 36.21±0.62%, which closely agreed with the predicted value of 35.75%.					
29412175	8	70	with	fucose	1422:1427	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	0	71	theme	Tratt	79:83	arg1	extraction					49:58	the microwave-assisted enzymatic extraction	16:58	the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt	16:83	Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt.					
29412175	2	72	theme	microwave-assisted	227:244	arg1	treatment					256:264	microwave-assisted enzymatic treatment	227:264	microwave-assisted enzymatic treatment	227:264	An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology.					
29412175	8	73	theme	glucuronic	1352:1361	arg1	acid					1363:1366	glucuronic acid	1352:1366	glucuronic acid	1352:1366	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	5	74	with	Purification	756:767	arg1	column					794:799	a DEAE-52 cellulose column	774:799	a DEAE-52 cellulose column	774:799	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
29412175	1	75	theme	α-d-glucosidase	161:175	arg1	activity					188:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity.					
29412175	8	76	with	glucose	1388:1394	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	3	77	theme	PB	416:417	arg1	design					420:425	Plackett-Burman (PB) design	399:425	Plackett-Burman (PB) design	399:425	The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield.					
29412175	6	78	theme	inhibitory	950:959	arg1	activity					961:968	α-d-glucosidase inhibitory activity	934:968	α-d-glucosidase inhibitory activity	934:968	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	6	79	theme	antioxidant	1045:1055	arg1	activity					1057:1064	stronger antioxidant activity	1036:1064	stronger antioxidant activity	1036:1064	Subsequently, the antioxidant activity and α-d-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α-d-glucosidase inhibitory activity than PR-2.					
29412175	8	80	with	mannose	1298:1304	arg1	percentages					1440:1450	molar percentages	1434:1450	molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively	1434:1536	The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.					
29412175	1	81	theme	inhibitory	177:186	arg1	activity					188:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	its antioxidant and α-d-glucosidase inhibitory activity	141:195	polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity.					
29412175	5	82	theme	DEAE-52	776:782	arg1	column					794:799	a DEAE-52 cellulose column	774:799	a DEAE-52 cellulose column	774:799	Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2×103 to 7.4KDa) and PR-2 (from 559.8 to 106.6KDa).					
31040189	8	0	theme	capillary	1203:1211	arg1	lumen					1213:1217	expanded capillary lumen	1194:1217	expanded capillary lumen	1194:1217	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	11	1	dep	homeostasis	1636:1646	arg1	development					1657:1667	development	1657:1667	development	1657:1667	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	5	2	theme	podocyte-specific	650:666	arg1	PCmas					703:707	PCmas	703:707	PCmas	703:707	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	5	2	theme	podocyte-specific	650:666	arg1	model					696:700	a podocyte-specific sialylation-deficient mouse model	648:700	a podocyte-specific sialylation-deficient mouse model (PCmas )	648:709	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	7	3	dep	RESULTS	954:960	arg1	resulted					1008:1015	resulted	1008:1015	resulted	1008:1015	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	5	4	theme	mouse	690:694	arg1	PCmas					703:707	PCmas	703:707	PCmas	703:707	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	5	4	theme	mouse	690:694	arg1	model					696:700	a podocyte-specific sialylation-deficient mouse model	648:700	a podocyte-specific sialylation-deficient mouse model (PCmas )	648:709	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	8	5	theme	synechiae	1248:1256	arg1	formation					1258:1266	synechiae formation	1248:1266	synechiae formation	1248:1266	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	11	6	theme	podocyte	1627:1634	arg1	homeostasis					1636:1646	podocyte homeostasis	1627:1646	podocyte homeostasis	1627:1646	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	2	7	theme	endothelial	322:332	arg1	glycocalyx					334:343	the glomerular endothelial glycocalyx	307:343	the glomerular endothelial glycocalyx	307:343	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	13	8	theme	complex	1855:1861	arg1	process					1863:1869	the complex process	1851:1869	the complex process causing FSGS	1851:1882	Our data support the notion that loss of sialylation might be part of the complex process causing FSGS.					
31040189	7	9	theme	diaphragms	1122:1131	arg1	effacement					1094:1103	foot process effacement	1081:1103	foot process effacement	1081:1103	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	7	9	theme	diaphragms	1122:1131	arg1	loss					1109:1112	loss	1109:1112	loss of slit diaphragms	1109:1131	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	14	10	theme	therapeutic	1965:1975	arg1	strategy					1977:1984	a new therapeutic strategy	1959:1984	a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies	1959:2045	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	8	11	theme	podocyte	1273:1280	arg1	loss					1282:1285	podocyte loss	1273:1285	podocyte loss	1273:1285	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	13	12	theme	sialylation	1822:1832	arg1	loss					1814:1817	loss	1814:1817	loss of sialylation	1814:1832	Our data support the notion that loss of sialylation might be part of the complex process causing FSGS.					
31040189	2	13	theme	underestimated	233:246	arg1	factor					248:253	an often underestimated factor	224:253	an often underestimated factor for pathologic processes	224:278	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	2	13	theme	underestimated	233:246	arg1	glycosylation					207:219	Aberrant glycosylation	198:219	Aberrant glycosylation	198:219	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	4	14	gly	sialylated	546:555	arg1	podocytes					525:533	Human podocytes	519:533	Human podocytes	519:533	Human podocytes are highly sialylated; however, sialylation's role in podocyte homeostasis remains unclear.					
31040189	1	15	theme	syndrome	133:140	arg1	etiology					93:100	The etiology	89:100	The etiology of steroid-resistant nephrotic syndrome, which manifests as FSGS,	89:166	BACKGROUND The etiology of steroid-resistant nephrotic syndrome, which manifests as FSGS, is not completely understood.					
31040189	12	16	theme	Impaired	1670:1677	arg1	adhesion					1688:1695	Impaired podocyte adhesion	1670:1695	Impaired podocyte adhesion to the glomerular basement membrane	1670:1731	Impaired podocyte adhesion to the glomerular basement membrane most likely contributed to disease development.					
31040189	7	17	theme	process	1086:1092	arg1	effacement					1094:1103	foot process effacement	1081:1103	foot process effacement	1081:1103	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	7	18	theme	postnatal	1048:1056	arg1	day					1058:1060	postnatal day 28	1048:1063	postnatal day 28	1048:1063	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	11	19	theme	CONCLUSIONS	1547:1557	arg1	Loss					1559:1562	CONCLUSIONS Loss	1547:1562	CONCLUSIONS Loss of cell-surface sialylation in mice	1547:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	5	20	theme	ultrastructural	768:782	arg1	analysis					784:791	histologic and ultrastructural analysis	753:791	histologic and ultrastructural analysis	753:791	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	7	21	theme	proteinuria	1029:1039	arg1	onset					1020:1024	onset	1020:1024	onset of proteinuria around postnatal day 28	1020:1063	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	14	22	theme	Sia	1916:1918	arg1	therapy					1936:1942	a Sia supplementation therapy	1914:1942	a Sia supplementation therapy	1914:1942	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	10	23	theme	impaired	1513:1520	arg1	adhesion					1522:1529	impaired adhesion	1513:1529	impaired adhesion to collagen IV	1513:1544	In vitro, asialo-podocytes were viable, able to proliferate and differentiate, but showed impaired adhesion to collagen IV.					
31040189	9	24	theme	slit	1348:1351	arg1	diaphragm					1353:1361	slit diaphragm	1348:1361	slit diaphragm components	1348:1372	In vivo, loss of sialylation resulted in mislocalization of slit diaphragm components, whereas podocalyxin localization was preserved.					
31040189	5	25	used	used	748:751	arg2	We					635:636	We	635:636	We	635:636	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	11	26	theme	sialylation	1580:1590	arg1	Loss					1559:1562	CONCLUSIONS Loss	1547:1562	CONCLUSIONS Loss of cell-surface sialylation in mice	1547:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	4	27	from	role	581:584	arg1	homeostasis					598:608	podocyte homeostasis	589:608	podocyte homeostasis	589:608	Human podocytes are highly sialylated; however, sialylation's role in podocyte homeostasis remains unclear.					
31040189	9	28	theme	components	1363:1372	arg1	mislocalization					1329:1343	mislocalization	1329:1343	mislocalization of slit diaphragm components	1329:1372	In vivo, loss of sialylation resulted in mislocalization of slit diaphragm components, whereas podocalyxin localization was preserved.					
31040189	11	29	from	sialylation	1580:1590	arg1	mice					1595:1598	mice	1595:1598	mice	1595:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	5	30	theme	histologic	753:762	arg1	analysis					784:791	histologic and ultrastructural analysis	753:791	histologic and ultrastructural analysis	753:791	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	7	31	theme	PCmas	997:1001	arg1	mice					1003:1006	PCmas mice	997:1006	PCmas mice	997:1006	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	11	32	from	mice	1595:1598	arg1	Loss					1559:1562	CONCLUSIONS Loss	1547:1562	CONCLUSIONS Loss of cell-surface sialylation in mice	1547:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	0	33	theme	Podocyte-Specific	0:16	arg1	Mice					40:43	Podocyte-Specific Sialylation-Deficient Mice	0:43	Podocyte-Specific Sialylation-Deficient Mice	0:43	Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.					
31040189	0	33	theme	Podocyte-Specific	0:16	arg1	Model					56:60	a Model	54:60	a Model for Human FSGS	54:75	Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.					
31040189	7	34	theme	sialylation	982:992	arg1	loss					974:977	Progressive loss	962:977	Progressive loss of sialylation in PCmas mice	962:1006	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	7	35	from	loss	974:977	arg1	mice					1003:1006	PCmas mice	997:1006	PCmas mice	997:1006	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	1	36	theme	steroid-resistant	105:121	arg1	syndrome					133:140	steroid-resistant nephrotic syndrome	105:140	steroid-resistant nephrotic syndrome	105:140	BACKGROUND The etiology of steroid-resistant nephrotic syndrome, which manifests as FSGS, is not completely understood.					
31040189	3	37	theme	sialic	434:439	arg1	Sia					447:449	Sia	447:449	Sia	447:449	Glycans are frequently capped by sialic acid (Sia), and sialylation's crucial role for kidney function is well known.					
31040189	3	37	theme	sialic	434:439	arg1	acid					441:444	sialic acid	434:444	sialic acid (Sia)	434:450	Glycans are frequently capped by sialic acid (Sia), and sialylation's crucial role for kidney function is well known.					
31040189	4	38	theme	Human	519:523	arg1	podocytes					525:533	Human podocytes	519:533	Human podocytes	519:533	Human podocytes are highly sialylated; however, sialylation's role in podocyte homeostasis remains unclear.					
31040189	0	39	theme	Human	66:70	arg1	FSGS					72:75	Human FSGS	66:75	Human FSGS	66:75	Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.					
31040189	3	40	theme	kidney	488:493	arg1	function					495:502	kidney function	488:502	kidney function	488:502	Glycans are frequently capped by sialic acid (Sia), and sialylation's crucial role for kidney function is well known.					
31040189	6	41	theme	sialylation-deficient	879:899	arg1	asialo-podocytes					912:927	asialo-podocytes	912:927	asialo-podocytes	912:927	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	6	41	theme	sialylation-deficient	879:899	arg1	podocytes					901:909	immortalized sialylation-deficient podocytes	866:909	immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies	866:951	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	8	42	theme	glomerular	1164:1173	arg1	formation					1258:1266	synechiae formation	1248:1266	synechiae formation	1248:1266	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	42	theme	glomerular	1164:1173	arg1	hypercellularity					1230:1245	mesangial hypercellularity	1220:1245	mesangial hypercellularity	1220:1245	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	42	theme	glomerular	1164:1173	arg1	loss					1282:1285	podocyte loss	1273:1285	podocyte loss	1273:1285	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	42	theme	glomerular	1164:1173	arg1	lumen					1213:1217	expanded capillary lumen	1194:1217	expanded capillary lumen	1194:1217	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	42	theme	glomerular	1164:1173	arg1	defects					1175:1181	severe glomerular defects	1157:1181	severe glomerular defects	1157:1181	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	4	43	theme	podocyte	589:596	arg1	homeostasis					598:608	podocyte homeostasis	589:608	podocyte homeostasis	589:608	Human podocytes are highly sialylated; however, sialylation's role in podocyte homeostasis remains unclear.					
31040189	2	44	theme	syndrome	391:398	arg1	models					371:376	models	371:376	models of nephrotic syndrome	371:398	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	7	45	theme	Progressive	962:972	arg1	loss					974:977	Progressive loss	962:977	Progressive loss of sialylation in PCmas mice	962:1006	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	8	46	theme	expanded	1194:1201	arg1	lumen					1213:1217	expanded capillary lumen	1194:1217	expanded capillary lumen	1194:1217	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	13	47	theme	process	1863:1869	arg1	part					1843:1846	part	1843:1846	part of the complex process causing FSGS	1843:1882	Our data support the notion that loss of sialylation might be part of the complex process causing FSGS.					
31040189	5	48	theme	sialylation-deficient	668:688	arg1	PCmas					703:707	PCmas	703:707	PCmas	703:707	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	5	48	theme	sialylation-deficient	668:688	arg1	model					696:700	a podocyte-specific sialylation-deficient mouse model	648:700	a podocyte-specific sialylation-deficient mouse model (PCmas )	648:709	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	6	49	theme	CRISPR/Cas9	831:841	arg1	technology					843:852	CRISPR/Cas9 technology	831:852	CRISPR/Cas9 technology	831:852	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	2	50	theme	structural	285:294	arg1	changes					296:302	structural changes	285:302	structural changes in the glomerular endothelial glycocalyx	285:343	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	14	51	theme	delay	1997:2001	arg1	FSGS					2003:2006	delay FSGS	1997:2006	delay FSGS	1997:2006	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	8	52	theme	mesangial	1220:1228	arg1	hypercellularity					1230:1245	mesangial hypercellularity	1220:1245	mesangial hypercellularity	1220:1245	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	6	53	theme	functional	934:943	arg1	studies					945:951	functional studies	934:951	functional studies	934:951	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	11	54	theme	FSGS	1652:1655	arg1	disturbance					1612:1622	disturbance	1612:1622	disturbance of podocyte homeostasis and FSGS development	1612:1667	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	7	55	theme	slit	1117:1120	arg1	diaphragms					1122:1131	slit diaphragms	1117:1131	slit diaphragms	1117:1131	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	11	56	theme	homeostasis	1636:1646	arg1	disturbance					1612:1622	disturbance	1612:1622	disturbance of podocyte homeostasis and FSGS development	1612:1667	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	2	57	theme	glomerular	311:320	arg1	glycocalyx					334:343	the glomerular endothelial glycocalyx	307:343	the glomerular endothelial glycocalyx	307:343	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	12	58	theme	glomerular	1704:1713	arg1	membrane					1724:1731	the glomerular basement membrane	1700:1731	the glomerular basement membrane	1700:1731	Impaired podocyte adhesion to the glomerular basement membrane most likely contributed to disease development.					
31040189	11	59	gly	sialylation	1580:1590	arg1	mice					1595:1598	mice	1595:1598	mice	1595:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	14	60	theme	new	1961:1963	arg1	strategy					1977:1984	a new therapeutic strategy	1959:1984	a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies	1959:2045	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	1	61	theme	nephrotic	123:131	arg1	syndrome					133:140	steroid-resistant nephrotic syndrome	105:140	steroid-resistant nephrotic syndrome	105:140	BACKGROUND The etiology of steroid-resistant nephrotic syndrome, which manifests as FSGS, is not completely understood.					
31040189	12	62	theme	podocyte	1679:1686	arg1	adhesion					1688:1695	Impaired podocyte adhesion	1670:1695	Impaired podocyte adhesion to the glomerular basement membrane	1670:1731	Impaired podocyte adhesion to the glomerular basement membrane most likely contributed to disease development.					
31040189	1	63	dep	BACKGROUND	78:87	arg1	understood					186:195	understood	186:195	is not completely understood	168:195	BACKGROUND The etiology of steroid-resistant nephrotic syndrome, which manifests as FSGS, is not completely understood.					
31040189	7	64	dep	resulted	1008:1015	arg1	accompanied					1066:1076	accompanied	1066:1076	accompanied by foot process effacement and loss of slit diaphragms	1066:1131	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	7	65	theme	foot	1081:1084	arg1	effacement					1094:1103	foot process effacement	1081:1103	foot process effacement	1081:1103	RESULTS Progressive loss of sialylation in PCmas mice resulted in onset of proteinuria around postnatal day 28, accompanied by foot process effacement and loss of slit diaphragms.					
31040189	9	66	theme	sialylation	1305:1315	arg1	loss					1297:1300	loss	1297:1300	loss of sialylation	1297:1315	In vivo, loss of sialylation resulted in mislocalization of slit diaphragm components, whereas podocalyxin localization was preserved.					
31040189	2	67	theme	pathologic	259:268	arg1	processes					270:278	pathologic processes	259:278	pathologic processes	259:278	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	14	68	theme	supplementation	1920:1934	arg1	therapy					1936:1942	a Sia supplementation therapy	1914:1942	a Sia supplementation therapy	1914:1942	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	2	69	from	changes	296:302	arg1	glycocalyx					334:343	the glomerular endothelial glycocalyx	307:343	the glomerular endothelial glycocalyx	307:343	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	11	70	theme	cell-surface	1567:1578	arg1	sialylation					1580:1590	cell-surface sialylation	1567:1590	cell-surface sialylation in mice	1567:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	9	71	theme	diaphragm	1353:1361	arg1	components					1363:1372	slit diaphragm components	1348:1372	slit diaphragm components	1348:1372	In vivo, loss of sialylation resulted in mislocalization of slit diaphragm components, whereas podocalyxin localization was preserved.					
31040189	0	72	theme	Sialylation-Deficient	18:38	arg1	Mice					40:43	Podocyte-Specific Sialylation-Deficient Mice	0:43	Podocyte-Specific Sialylation-Deficient Mice	0:43	Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.					
31040189	0	72	theme	Sialylation-Deficient	18:38	arg1	Model					56:60	a Model	54:60	a Model for Human FSGS	54:75	Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.					
31040189	9	73	theme	podocalyxin	1383:1393	arg1	localization					1395:1406	podocalyxin localization	1383:1406	podocalyxin localization	1383:1406	In vivo, loss of sialylation resulted in mislocalization of slit diaphragm components, whereas podocalyxin localization was preserved.					
31040189	12	74	theme	disease	1760:1766	arg1	development					1768:1778	disease development	1760:1778	disease development	1760:1778	Impaired podocyte adhesion to the glomerular basement membrane most likely contributed to disease development.					
31040189	5	75	theme	CMP-Sia	724:730	arg1	synthetase					732:741	CMP-Sia synthetase	724:741	CMP-Sia synthetase	724:741	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	8	76	theme	Podocyte	1134:1141	arg1	injury					1143:1148	Podocyte injury	1134:1148	Podocyte injury	1134:1148	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	11	77	from	Loss	1559:1562	arg1	mice					1595:1598	mice	1595:1598	mice	1595:1598	CONCLUSIONS Loss of cell-surface sialylation in mice resulted in disturbance of podocyte homeostasis and FSGS development.					
31040189	12	78	theme	basement	1715:1722	arg1	membrane					1724:1731	the glomerular basement membrane	1700:1731	the glomerular basement membrane	1700:1731	Impaired podocyte adhesion to the glomerular basement membrane most likely contributed to disease development.					
31040189	10	79	from	vitro	1426:1430	arg1	viable					1455:1460	viable	1455:1460	viable	1455:1460	In vitro, asialo-podocytes were viable, able to proliferate and differentiate, but showed impaired adhesion to collagen IV.					
31040189	3	80	theme	crucial	471:477	arg1	role					479:482	sialylation's crucial role	457:482	sialylation's crucial role for kidney function	457:502	Glycans are frequently capped by sialic acid (Sia), and sialylation's crucial role for kidney function is well known.					
31040189	8	81	theme	severe	1157:1162	arg1	formation					1258:1266	synechiae formation	1248:1266	synechiae formation	1248:1266	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	81	theme	severe	1157:1162	arg1	hypercellularity					1230:1245	mesangial hypercellularity	1220:1245	mesangial hypercellularity	1220:1245	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	81	theme	severe	1157:1162	arg1	loss					1282:1285	podocyte loss	1273:1285	podocyte loss	1273:1285	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	81	theme	severe	1157:1162	arg1	lumen					1213:1217	expanded capillary lumen	1194:1217	expanded capillary lumen	1194:1217	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	8	81	theme	severe	1157:1162	arg1	defects					1175:1181	severe glomerular defects	1157:1181	severe glomerular defects	1157:1181	Podocyte injury led to severe glomerular defects, including expanded capillary lumen, mesangial hypercellularity, synechiae formation, and podocyte loss.					
31040189	10	82	from	viable	1455:1460	arg1	vitro					1426:1430	vitro	1426:1430	vitro	1426:1430	In vitro, asialo-podocytes were viable, able to proliferate and differentiate, but showed impaired adhesion to collagen IV.					
31040189	5	83	dep	METHODS	627:633	arg1	used					748:751	used	748:751	used histologic and ultrastructural analysis to decipher the phenotype	748:817	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	5	83	dep	METHODS	627:633	arg1	generated					638:646	generated	638:646	generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase	638:741	METHODS We generated a podocyte-specific sialylation-deficient mouse model (PCmas ) by targeting CMP-Sia synthetase, and used histologic and ultrastructural analysis to decipher the phenotype.					
31040189	6	84	theme	immortalized	866:877	arg1	asialo-podocytes					912:927	asialo-podocytes	912:927	asialo-podocytes	912:927	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	6	84	theme	immortalized	866:877	arg1	podocytes					901:909	immortalized sialylation-deficient podocytes	866:909	immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies	866:951	We applied CRISPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for functional studies.					
31040189	14	85	theme	other	2024:2028	arg1	glomerulopathies					2030:2045	potentially other glomerulopathies	2012:2045	potentially other glomerulopathies	2012:2045	Sialylation, such as through a Sia supplementation therapy, might provide a new therapeutic strategy to cure or delay FSGS and potentially other glomerulopathies.					
31040189	2	86	theme	Aberrant	198:205	arg1	factor					248:253	an often underestimated factor	224:253	an often underestimated factor for pathologic processes	224:278	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	2	86	theme	Aberrant	198:205	arg1	glycosylation					207:219	Aberrant glycosylation	198:219	Aberrant glycosylation	198:219	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	2	87	theme	nephrotic	381:389	arg1	syndrome					391:398	nephrotic syndrome	381:398	nephrotic syndrome	381:398	Aberrant glycosylation is an often underestimated factor for pathologic processes, and structural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic syndrome.					
31040189	10	88	dep	viable	1455:1460	arg1	able					1463:1466	able	1463:1466	able	1463:1466	In vitro, asialo-podocytes were viable, able to proliferate and differentiate, but showed impaired adhesion to collagen IV.					
30500162	0	0	theme	Antibodies	79:88	arg1	Recognition					36:46	Recognition	36:46	Recognition of Human Serum anti-Neu5Gc IgG Antibodies	36:88	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	4	1	link	meat-derived	576:587	arg1	monosaccharide					589:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	8	2	theme	First	1092:1096	arg1	strains					1141:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	7	3	theme	glycoproteins	1039:1051	arg1	recognition					1006:1016	serum IgG recognition	996:1016	serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans	996:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	1	4	theme	antigen	153:159	arg1	composition					161:171	antigen composition	153:171	antigen composition	153:171	Recognition of carbohydrates by antibodies can be affected by antigen composition and density.					
30500162	11	5	theme	collection	1644:1653	arg1	detection					1622:1630	detection	1622:1630	detection of the full collection of human serum anti-Neu5Gc IgGs	1622:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	8	6	from	glycoproteins	1196:1208	arg1	glycopeptides					1171:1183	glycopeptides	1171:1183	glycopeptides from serum glycoproteins	1171:1208	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	0	7	theme	IgG	75:77	arg1	Antibodies					79:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	6	8	theme	human	966:970	arg1	serum					972:976	human serum	966:976	human serum	966:976	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	11	9	theme	serum	1664:1668	arg1	IgGs					1682:1685	human serum anti-Neu5Gc IgGs	1658:1685	human serum anti-Neu5Gc IgGs	1658:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	9	10	theme	anti-Neu5Gc	1338:1348	arg1	IgGs					1350:1353	human serum anti-Neu5Gc IgGs	1326:1353	human serum anti-Neu5Gc IgGs	1326:1353	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	5	11	theme	diseases	768:775	arg1	exacerbation					752:763	exacerbation	752:763	exacerbation of diseases mediated by chronic inflammation such as cancer and atherosclerosis	752:843	Such antibodies can cause exacerbation of diseases mediated by chronic inflammation such as cancer and atherosclerosis.					
30500162	8	12	theme	Neu5Gc-positive	1099:1113	arg1	strains					1141:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	9	13	theme	human	1260:1264	arg1	sera					1266:1269	human sera	1260:1269	human sera against Neu5Gc-positive glycopeptides	1260:1307	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	2	14	theme	such	305:308	arg1	effects					310:316	such effects	305:316	such effects on anticarbohydrate antibodies in circulating human serum	305:374	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	6	15	from	detection	933:941	arg1	serum					972:976	human serum	966:976	human serum	966:976	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	6	16	theme	IgGs	958:961	arg1	detection					933:941	the detection	929:941	the detection of anti-Neu5Gc IgGs in human serum	929:976	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	7	17	theme	serum	996:1000	arg1	recognition					1006:1016	serum IgG recognition	996:1016	serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans	996:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	11	18	theme	presentation	1555:1566	arg1	mode					1568:1571	the presentation mode	1551:1571	the presentation mode	1551:1571	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	11	18	theme	presentation	1555:1566	arg1	critical					1609:1616	critical	1609:1616	critical	1609:1616	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	3	19	theme	sialic	513:518	arg1	form					525:528	a nonhuman sialic acid form	502:528	a nonhuman sialic acid form	502:528	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	19	theme	sialic	513:518	arg1	acid					552:555	N-glycolylneuraminic acid	531:555	N-glycolylneuraminic acid (Neu5Gc)	531:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	8	20	theme	Neu5Gc-deficient	1118:1133	arg1	strains					1141:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	7	21	theme	IgG	1002:1004	arg1	recognition					1006:1016	serum IgG recognition	996:1016	serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans	996:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	10	22	theme	ELISA	1378:1382	arg1	screens					1384:1390	ELISA screens	1378:1390	ELISA screens	1378:1390	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	5	23	theme	chronic	789:795	arg1	inflammation					797:808	chronic inflammation	789:808	chronic inflammation such as cancer and atherosclerosis	789:843	Such antibodies can cause exacerbation of diseases mediated by chronic inflammation such as cancer and atherosclerosis.					
30500162	11	24	theme	full	1639:1642	arg1	collection					1644:1653	the full collection	1635:1653	the full collection of human serum anti-Neu5Gc IgGs	1635:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	9	25	theme	Neu5Gc-positive	1279:1293	arg1	glycopeptides					1295:1307	Neu5Gc-positive glycopeptides	1279:1307	Neu5Gc-positive glycopeptides	1279:1307	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	2	26	theme	carbohydrate	278:289	arg1	antigens					291:298	synthetic carbohydrate antigens	268:298	synthetic carbohydrate antigens	268:298	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	9	27	theme	serum	1332:1336	arg1	IgGs					1350:1353	human serum anti-Neu5Gc IgGs	1326:1353	human serum anti-Neu5Gc IgGs	1326:1353	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	3	28	theme	diverse	452:458	arg1	response					460:467	a polyclonal and diverse response	435:467	a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc)	435:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	4	29	theme	meat-derived	576:587	arg1	monosaccharide					589:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	10	30	with	comparison	1417:1426	arg1	glycoproteins					1433:1445	glycoproteins	1433:1445	glycoproteins	1433:1445	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	7	31	gly	glycopeptides	1054:1066	arg2	glycopeptides					1054:1066	glycopeptides	1054:1066	glycopeptides	1054:1066	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	2	32	theme	anticarbohydrate	321:336	arg1	antibodies					338:347	anticarbohydrate antibodies	321:347	anticarbohydrate antibodies	321:347	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	6	33	theme	anti-Neu5Gc	946:956	arg1	IgGs					958:961	anti-Neu5Gc IgGs	946:961	anti-Neu5Gc IgGs	946:961	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	4	34	theme	human	714:718	arg1	sera					720:723	human sera	714:723	human sera	714:723	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	0	35	theme	Presentation	0:11	arg1	Mode					13:16	Presentation Mode	0:16	Presentation Mode of Glycans	0:27	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	4	36	theme	red	572:574	arg1	monosaccharide					589:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide	567:602	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	8	37	theme	mouse	1135:1139	arg1	strains					1141:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	11	38	theme	IgGs	1682:1685	arg1	collection					1644:1653	the full collection	1635:1653	the full collection of human serum anti-Neu5Gc IgGs	1635:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	8	39	gly	glycopeptides	1171:1183	arg2	glycopeptides					1171:1183	glycopeptides	1171:1183	glycopeptides from serum glycoproteins	1171:1208	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	8	39	gly	glycopeptides	1171:1183	arg1	glycoproteins					1196:1208	serum glycoproteins	1190:1208	serum glycoproteins	1190:1208	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	3	40	theme	polyclonal	437:446	arg1	response					460:467	a polyclonal and diverse response	435:467	a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc)	435:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	0	41	theme	Glycans	21:27	arg1	Mode					13:16	Presentation Mode	0:16	Presentation Mode of Glycans	0:27	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	11	42	theme	human	1658:1662	arg1	IgGs					1682:1685	human serum anti-Neu5Gc IgGs	1658:1685	human serum anti-Neu5Gc IgGs	1658:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	11	43	theme	Neu5Gc-glycans	1590:1603	arg1	diversity					1577:1585	diversity	1577:1585	diversity of Neu5Gc-glycans	1577:1603	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	11	43	theme	Neu5Gc-glycans	1590:1603	arg1	mode					1568:1571	the presentation mode	1551:1571	the presentation mode	1551:1571	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	11	43	theme	Neu5Gc-glycans	1590:1603	arg1	critical					1609:1616	critical	1609:1616	critical	1609:1616	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	8	44	used	used	1154:1157	arg2	strains					1141:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains	1092:1147	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	7	45	gly	glycoproteins	1039:1051	arg1	glycoproteins					1039:1051	Neu5Gc-containing glycoproteins	1021:1051	Neu5Gc-containing glycoproteins	1021:1051	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	1	46	theme	carbohydrates	106:118	arg1	Recognition					91:101	Recognition	91:101	Recognition of carbohydrates by antibodies	91:132	Recognition of carbohydrates by antibodies can be affected by antigen composition and density.					
30500162	4	47	theme	glycans	655:661	arg1	collection					635:644	a diverse collection	625:644	a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera	625:723	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	9	48	gly	glycopeptides	1295:1307	arg2	glycopeptides					1295:1307	Neu5Gc-positive glycopeptides	1279:1307	Neu5Gc-positive glycopeptides	1279:1307	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	3	49	theme	acid	520:523	arg1	form					525:528	a nonhuman sialic acid form	502:528	a nonhuman sialic acid form	502:528	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	49	theme	acid	520:523	arg1	acid					552:555	N-glycolylneuraminic acid	531:555	N-glycolylneuraminic acid (Neu5Gc)	531:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	9	50	theme	human	1326:1330	arg1	IgGs					1350:1353	human serum anti-Neu5Gc IgGs	1326:1353	human serum anti-Neu5Gc IgGs	1326:1353	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	2	51	theme	controllable	229:240	arg1	systems					254:260	controllable multivalent systems	229:260	controllable multivalent systems	229:260	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	8	52	gly	glycoproteins	1196:1208	arg1	glycoproteins					1196:1208	serum glycoproteins	1190:1208	serum glycoproteins	1190:1208	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	11	53	theme	anti-Neu5Gc	1670:1680	arg1	IgGs					1682:1685	human serum anti-Neu5Gc IgGs	1658:1685	human serum anti-Neu5Gc IgGs	1658:1685	Our results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5Gc IgGs.					
30500162	10	54	gly	glycopeptides	1400:1412	arg2	glycopeptides					1400:1412	glycopeptides	1400:1412	glycopeptides in comparison with glycoproteins	1400:1445	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	3	55	contain	containing	491:500	arg2	acid					552:555	N-glycolylneuraminic acid	531:555	N-glycolylneuraminic acid (Neu5Gc)	531:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	55	contain	containing	491:500	arg1	carbohydrates					477:489	carbohydrates	477:489	carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc)	477:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	55	contain	containing	491:500	arg2	form					525:528	a nonhuman sialic acid form	502:528	a nonhuman sialic acid form	502:528	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	7	56	theme	synthetic	1073:1081	arg1	glycans					1083:1089	synthetic glycans	1073:1089	synthetic glycans	1073:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	9	57	theme	reproducible	1231:1242	arg1	ELISA					1244:1248	a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs	1229:1353	a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs	1229:1353	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	0	58	theme	Human	51:55	arg1	Antibodies					79:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	6	59	theme	Neu5Gc-glycans	903:916	arg1	modes					894:898	different presentation modes	871:898	different presentation modes of Neu5Gc-glycans	871:916	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	7	60	theme	glycans	1083:1089	arg1	recognition					1006:1016	serum IgG recognition	996:1016	serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans	996:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	2	61	theme	human	364:368	arg1	serum					370:374	circulating human serum	352:374	circulating human serum	352:374	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	10	62	gly	glycoproteins	1433:1445	arg1	glycoproteins					1433:1445	glycoproteins	1433:1445	glycoproteins	1433:1445	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	9	63	theme	IgGs	1350:1353	arg1	detection					1313:1321	detection	1313:1321	detection of human serum anti-Neu5Gc IgGs	1313:1353	Then we developed a reproducible ELISA to screen human sera against Neu5Gc-positive glycopeptides for detection of human serum anti-Neu5Gc IgGs.					
30500162	2	64	theme	circulating	352:362	arg1	serum					370:374	circulating human serum	352:374	circulating human serum	352:374	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	5	65	theme	Such	726:729	arg1	antibodies					731:740	Such antibodies	726:740	Such antibodies	726:740	Such antibodies can cause exacerbation of diseases mediated by chronic inflammation such as cancer and atherosclerosis.					
30500162	10	66	from	glycopeptides	1400:1412	arg1	comparison					1417:1426	comparison	1417:1426	comparison with glycoproteins	1417:1445	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	2	67	theme	synthetic	268:276	arg1	antigens					291:298	synthetic carbohydrate antigens	268:298	synthetic carbohydrate antigens	268:298	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	10	68	theme	synthetic	1496:1504	arg1	Neu5Gc-glycans					1506:1519	synthetic Neu5Gc-glycans	1496:1519	synthetic Neu5Gc-glycans	1496:1519	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	8	69	theme	serum	1190:1194	arg1	glycoproteins					1196:1208	serum glycoproteins	1190:1208	serum glycoproteins	1190:1208	First, Neu5Gc-positive or Neu5Gc-deficient mouse strains were used to generate glycopeptides from serum glycoproteins.					
30500162	6	70	theme	presentation	881:892	arg1	modes					894:898	different presentation modes	871:898	different presentation modes of Neu5Gc-glycans	871:916	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	10	71	theme	elaborated	1467:1476	arg1	arrays					1478:1483	elaborated arrays	1467:1483	elaborated arrays displaying synthetic Neu5Gc-glycans	1467:1519	Finally, we evaluated ELISA screens against glycopeptides in comparison with glycoproteins, as well as against elaborated arrays displaying synthetic Neu5Gc-glycans.					
30500162	3	72	theme	nonhuman	504:511	arg1	form					525:528	a nonhuman sialic acid form	502:528	a nonhuman sialic acid form	502:528	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	72	theme	nonhuman	504:511	arg1	acid					552:555	N-glycolylneuraminic acid	531:555	N-glycolylneuraminic acid (Neu5Gc)	531:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	4	73	theme	diverse	627:633	arg1	collection					635:644	a diverse collection	625:644	a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera	625:723	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	0	74	theme	anti-Neu5Gc	63:73	arg1	Antibodies					79:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	6	75	theme	different	871:879	arg1	modes					894:898	different presentation modes	871:898	different presentation modes of Neu5Gc-glycans	871:916	We aimed to evaluate how different presentation modes of Neu5Gc-glycans can affect the detection of anti-Neu5Gc IgGs in human serum.					
30500162	2	76	theme	systems	254:260	arg1	systems					254:260	controllable multivalent systems	229:260	controllable multivalent systems	229:260	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	2	76	theme	systems	254:260	arg1	variety					218:224	a variety	216:224	a variety of controllable multivalent systems	216:260	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	7	77	theme	glycopeptides	1054:1066	arg1	recognition					1006:1016	serum IgG recognition	996:1016	serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans	996:1089	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	3	78	theme	N-glycolylneuraminic	531:550	arg1	Neu5Gc					558:563	Neu5Gc	558:563	Neu5Gc	558:563	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	78	theme	N-glycolylneuraminic	531:550	arg1	form					525:528	a nonhuman sialic acid form	502:528	a nonhuman sialic acid form	502:528	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	3	78	theme	N-glycolylneuraminic	531:550	arg1	acid					552:555	N-glycolylneuraminic acid	531:555	N-glycolylneuraminic acid (Neu5Gc)	531:564	All humans develop a polyclonal and diverse response against carbohydrates containing a nonhuman sialic acid form, N-glycolylneuraminic acid (Neu5Gc).					
30500162	4	79	theme	anti-Neu5Gc	688:698	arg1	antibodies					700:709	anti-Neu5Gc antibodies	688:709	anti-Neu5Gc antibodies	688:709	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	4	80	theme	human	649:653	arg1	glycans					655:661	human glycans	649:661	human glycans resulting in circulating anti-Neu5Gc antibodies in human sera	649:723	This red meat-derived monosaccharide is incorporated into a diverse collection of human glycans resulting in circulating anti-Neu5Gc antibodies in human sera.					
30500162	0	81	theme	Serum	57:61	arg1	Antibodies					79:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Human Serum anti-Neu5Gc IgG Antibodies	51:88	Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.					
30500162	2	82	theme	multivalent	242:252	arg1	systems					254:260	controllable multivalent systems	229:260	controllable multivalent systems	229:260	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	7	83	theme	Neu5Gc-containing	1021:1037	arg1	glycoproteins					1039:1051	Neu5Gc-containing glycoproteins	1021:1051	Neu5Gc-containing glycoproteins	1021:1051	Here, we compare serum IgG recognition of Neu5Gc-containing glycoproteins, glycopeptides, and synthetic glycans.					
30500162	2	84	from	effects	310:316	arg1	antibodies					338:347	anticarbohydrate antibodies	321:347	anticarbohydrate antibodies	321:347	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30500162	2	84	from	effects	310:316	arg1	serum					370:374	circulating human serum	352:374	circulating human serum	352:374	This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.					
30554514	7	0	with	interaction	1038:1048	arg1	microfibrils					1069:1080	microfibrils	1069:1080	microfibrils	1069:1080	Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces.					
30554514	5	1	theme	similar	730:736	arg1	changes					738:744	similar changes	730:744	similar changes	730:744	Glucomannan-cellulose underwent similar changes to cellulose, except that the macrofibrillar network was more entangled after DAP.					
30554514	2	2	theme	X-ray	408:412	arg1	diffraction					414:424	X-ray diffraction	408:424	X-ray diffraction	408:424	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	3	3	theme	macrofibrillar	577:590	arg1	network					592:598	macrofibrillar network	577:598	macrofibrillar network	577:598	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	2	4	theme	small-angle	376:386	arg1	scattering					396:405	small-angle neutron scattering	376:405	small-angle neutron scattering	376:405	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	8	5	theme	decreased	1307:1315	arg1	recalcitrance					1317:1329	decreased recalcitrance	1307:1329	decreased recalcitrance	1307:1329	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	7	6	theme	xyloglucan	1053:1062	arg1	interaction					1038:1048	a tight interaction	1030:1048	a tight interaction of xyloglucan with microfibrils	1030:1080	Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces.					
30554514	6	7	from	unchanged	935:943	arg1	xyloglucan-cellulose					844:863	xyloglucan-cellulose	844:863	xyloglucan-cellulose	844:863	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	6	8	theme	macrofibrillar	896:909	arg1	network					911:917	macrofibrillar network	896:917	macrofibrillar network	896:917	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	3	9	theme	native	603:608	arg1	cellulose					610:618	native cellulose	603:618	native cellulose	603:618	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	2	10	theme	sum	431:433	arg1	spectroscopy					456:467	sum frequency generation spectroscopy	431:467	sum frequency generation spectroscopy	431:467	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	2	11	theme	generation	445:454	arg1	spectroscopy					456:467	sum frequency generation spectroscopy	431:467	sum frequency generation spectroscopy	431:467	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	2	12	theme	frequency	435:443	arg1	spectroscopy					456:467	sum frequency generation spectroscopy	431:467	sum frequency generation spectroscopy	431:467	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	8	13	theme	hemicellulose-cellulose	1194:1216	arg1	interactions					1218:1229	different hemicellulose-cellulose interactions	1184:1229	different hemicellulose-cellulose interactions	1184:1229	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	8	14	theme	engineering	1283:1293	arg1	plants					1295:1300	engineering plants	1283:1300	engineering plants	1283:1300	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	1	15	theme	Model	112:116	arg1	composites					142:151	Model hemicellulose-cellulose composites	112:151	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions	112:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	1	16	theme	deuterated	231:240	arg1	cellulose					252:260	deuterated bacterial cellulose	231:260	deuterated bacterial cellulose	231:260	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	0	17	theme	Hemicellulose-Cellulose	0:22	arg1	Composites					24:33	Hemicellulose-Cellulose Composites	0:33	Hemicellulose-Cellulose Composites	0:33	Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.					
30554514	8	18	theme	different	1184:1192	arg1	interactions					1218:1229	different hemicellulose-cellulose interactions	1184:1229	different hemicellulose-cellulose interactions	1184:1229	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	6	19	theme	macrofibril	869:879	arg1	dimensions					881:890	the macrofibril dimensions	865:890	the macrofibril dimensions	865:890	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	6	19	theme	macrofibril	869:879	arg1	unchanged					935:943	unchanged	935:943	unchanged	935:943	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	1	20	theme	hemicellulose-cellulose	118:140	arg1	composites					142:151	Model hemicellulose-cellulose composites	112:151	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions	112:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	1	21	theme	bacterial	242:250	arg1	cellulose					252:260	deuterated bacterial cellulose	231:260	deuterated bacterial cellulose	231:260	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	5	22	theme	macrofibrillar	776:789	arg1	network					791:797	the macrofibrillar network	772:797	the macrofibrillar network	772:797	Glucomannan-cellulose underwent similar changes to cellulose, except that the macrofibrillar network was more entangled after DAP.					
30554514	5	22	theme	macrofibrillar	776:789	arg1	entangled					808:816	entangled	808:816	entangled	808:816	Glucomannan-cellulose underwent similar changes to cellulose, except that the macrofibrillar network was more entangled after DAP.					
30554514	7	23	theme	tight	1032:1036	arg1	interaction					1038:1048	a tight interaction	1030:1048	a tight interaction of xyloglucan with microfibrils	1030:1080	Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces.					
30554514	2	24	theme	materials	348:356	arg1	DAP					334:336	DAP	334:336	DAP	334:336	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	2	24	theme	materials	348:356	arg1	pretreatment					320:331	Dilute acid pretreatment	308:331	Dilute acid pretreatment (DAP) of these materials	308:356	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	4	25	theme	crystallite	638:648	arg1	dimension					655:663	the crystallite size dimension	634:663	the crystallite size dimension along the (010) plane	634:685	In addition, the crystallite size dimension along the (010) plane increased.					
30554514	8	26	theme	interactions	1218:1229	arg1	roles					1175:1179	roles	1175:1179	roles of different hemicellulose-cellulose interactions	1175:1229	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	6	27	theme	Iβ	983:984	arg1	content					986:992	the cellulose Iβ content	969:992	the cellulose Iβ content	969:992	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	3	28	theme	network	592:598	arg1	entanglement					561:572	entanglement	561:572	entanglement	561:572	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	0	29	theme	Cellulose	57:65	arg1	Organization					67:78	Cellulose Organization	57:78	Cellulose Organization	57:78	Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.					
30554514	2	30	theme	neutron	388:394	arg1	scattering					396:405	small-angle neutron scattering	376:405	small-angle neutron scattering	376:405	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	6	31	theme	cellulose	973:981	arg1	content					986:992	the cellulose Iβ content	969:992	the cellulose Iβ content	969:992	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	3	32	theme	cellulose	515:523	arg1	dimensions					486:495	The macrofibril dimensions	470:495	The macrofibril dimensions of the pretreated cellulose alone	470:529	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	3	32	theme	cellulose	515:523	arg1	smaller					536:542	smaller	536:542	smaller	536:542	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	3	33	theme	macrofibril	474:484	arg1	dimensions					486:495	The macrofibril dimensions	470:495	The macrofibril dimensions of the pretreated cellulose alone	470:529	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	3	33	theme	macrofibril	474:484	arg1	smaller					536:542	smaller	536:542	smaller	536:542	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	4	34	theme	size	650:653	arg1	dimension					655:663	the crystallite size dimension	634:663	the crystallite size dimension along the (010) plane	634:685	In addition, the crystallite size dimension along the (010) plane increased.					
30554514	7	35	theme	macrofibril	1120:1130	arg1	surfaces					1132:1139	macrofibril surfaces	1120:1139	macrofibril surfaces	1120:1139	Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces.					
30554514	1	36	theme	plant	164:168	arg1	interactions					188:199	plant cell wall polymer interactions	164:199	plant cell wall polymer interactions	164:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	6	37	from	xyloglucan-cellulose	844:863	arg1	dimensions					881:890	the macrofibril dimensions	865:890	the macrofibril dimensions	865:890	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	6	37	from	xyloglucan-cellulose	844:863	arg1	unchanged					935:943	unchanged	935:943	unchanged	935:943	Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose Iβ content was increased.					
30554514	1	38	theme	cell	170:173	arg1	interactions					188:199	plant cell wall polymer interactions	164:199	plant cell wall polymer interactions	164:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	4	39	theme	010	676:678	arg1	plane					681:685	the (010) plane	671:685	the (010) plane	671:685	In addition, the crystallite size dimension along the (010) plane increased.					
30554514	8	40	theme	pretreatment	1257:1268	arg1	processes					1270:1278	pretreatment processes	1257:1278	pretreatment processes	1257:1278	This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.					
30554514	2	41	theme	acid	315:318	arg1	DAP					334:336	DAP	334:336	DAP	334:336	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	2	41	theme	acid	315:318	arg1	pretreatment					320:331	Dilute acid pretreatment	308:331	Dilute acid pretreatment (DAP) of these materials	308:356	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	1	42	theme	wall	175:178	arg1	interactions					188:199	plant cell wall polymer interactions	164:199	plant cell wall polymer interactions	164:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	1	43	theme	glucomannan	281:291	arg1	presence					269:276	the presence	265:276	the presence of glucomannan or xyloglucan	265:305	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	0	44	theme	Acid	93:96	arg1	Pretreatment					98:109	Dilute Acid Pretreatment	86:109	Dilute Acid Pretreatment	86:109	Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.					
30554514	2	45	theme	Dilute	308:313	arg1	DAP					334:336	DAP	334:336	DAP	334:336	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	2	45	theme	Dilute	308:313	arg1	pretreatment					320:331	Dilute acid pretreatment	308:331	Dilute acid pretreatment (DAP) of these materials	308:356	Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy.					
30554514	1	46	theme	polymer	180:186	arg1	interactions					188:199	plant cell wall polymer interactions	164:199	plant cell wall polymer interactions	164:199	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30554514	3	47	theme	pretreated	504:513	arg1	cellulose					515:523	the pretreated cellulose	500:523	the pretreated cellulose alone	500:529	The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose.					
30554514	0	48	theme	Dilute	86:91	arg1	Pretreatment					98:109	Dilute Acid Pretreatment	86:109	Dilute Acid Pretreatment	86:109	Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.					
30554514	0	49	from	Differences	42:52	arg1	Organization					67:78	Cellulose Organization	57:78	Cellulose Organization	57:78	Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.					
30554514	1	50	theme	xyloglucan	296:305	arg1	presence					269:276	the presence	265:276	the presence of glucomannan or xyloglucan	265:305	Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan.					
30380738	9	0	with	GOS	1628:1630	arg1	linkage					1643:1649	β-1,4 linkage	1637:1649	β-1,4 linkage	1637:1649	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	9	0	with	GOS	1628:1630	arg1	linkages					1661:1668	β-1,6 linkages	1655:1668	β-1,6 linkages	1655:1668	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	6	1	theme	primary	1066:1072	arg1	structures					1074:1083	identical primary structures	1056:1083	identical primary structures	1056:1083	However, rMaBGA and wtMaBGA displayed different thermal stability and steady-state kinetics, although they share identical primary structures.					
30380738	0	2	from	Expression	13:22	arg1	Synthesis					83:91	Synthesis	83:91	Synthesis of Galactooligosaccharides	83:118	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	8	3	from	potential	1305:1313	arg1	synthesis					1332:1340	synthesis	1332:1340	synthesis of galactooligosaccharides (GOS)	1332:1373	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	0	4	from	Potential	70:78	arg1	Synthesis					83:91	Synthesis	83:91	Synthesis of Galactooligosaccharides	83:118	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	10	5	with	biosynthesis	1758:1769	arg1	linkage					1796:1802	β-1,3 linkage	1790:1802	β-1,3 linkage	1790:1802	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	8	6	theme	recombinant	1389:1399	arg1	enzyme					1401:1406	the purified recombinant enzyme	1376:1406	the purified recombinant enzyme	1376:1406	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	10	7	theme	recombinant	1702:1712	arg1	alternative					1742:1752	a promising alternative	1730:1752	a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage	1730:1802	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	10	7	theme	recombinant	1702:1712	arg1	enzyme					1714:1719	the recombinant enzyme	1698:1719	the recombinant enzyme	1698:1719	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	10	8	theme	β-1,3	1790:1794	arg1	linkage					1796:1802	β-1,3 linkage	1790:1802	β-1,3 linkage	1790:1802	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	8	9	theme	enzyme	1322:1327	arg1	potential					1305:1313	the potential	1301:1313	the potential of the enzyme in synthesis of galactooligosaccharides (GOS)	1301:1373	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	2	10	dep	Escherichia	231:241	arg1	coli					243:246	coli	243:246	coli	243:246	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	0	11	theme	Galactooligosaccharides	96:118	arg1	Synthesis					83:91	Synthesis	83:91	Synthesis of Galactooligosaccharides	83:118	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	10	12	theme	GOS	1774:1776	arg1	biosynthesis					1758:1769	biosynthesis	1758:1769	biosynthesis of GOS mainly with β-1,3 linkage	1758:1802	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	2	13	theme	recombinant	316:326	arg1	cells					328:332	the recombinant cells	312:332	the recombinant cells	312:332	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	7	14	theme	heterologous	1151:1162	arg1	expression					1164:1173	heterologous expression	1151:1173	heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics	1151:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	9	15	theme	β-1,6	1655:1659	arg1	linkages					1661:1668	β-1,6 linkages	1655:1668	β-1,6 linkages	1655:1668	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	6	16	theme	steady-state	1013:1024	arg1	kinetics					1026:1033	steady-state kinetics	1013:1033	steady-state kinetics	1013:1033	However, rMaBGA and wtMaBGA displayed different thermal stability and steady-state kinetics, although they share identical primary structures.					
30380738	8	17	theme	transgalactosylation	1437:1456	arg1	reaction					1458:1465	the transgalactosylation reaction	1433:1465	the transgalactosylation reaction	1433:1465	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	6	18	theme	different	981:989	arg1	stability					999:1007	different thermal stability	981:1007	different thermal stability	981:1007	However, rMaBGA and wtMaBGA displayed different thermal stability and steady-state kinetics, although they share identical primary structures.					
30380738	6	19	theme	identical	1056:1064	arg1	structures					1074:1083	identical primary structures	1056:1083	identical primary structures	1056:1083	However, rMaBGA and wtMaBGA displayed different thermal stability and steady-state kinetics, although they share identical primary structures.					
30380738	4	20	theme	specific	666:673	arg1	activity					675:682	The specific activity	662:682	The specific activity of the purified enzyme	662:705	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	4	20	theme	specific	666:673	arg1	mg-1					719:722	126.4 U mg-1	711:722	126.4 U mg-1	711:722	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	9	21	theme	products	1517:1524	arg1	One					1485:1487	One	1485:1487	One	1485:1487	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	9	21	theme	products	1517:1524	arg1	3'-galactosyl-lactose					1542:1562	3'-galactosyl-lactose	1542:1562	3'-galactosyl-lactose	1542:1562	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	9	21	theme	products	1517:1524	arg1	products					1517:1524	the transgalactosylation products	1492:1524	the transgalactosylation products	1492:1524	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	1	22	from	sp	173:174	arg1	β-1,3-galactosidase					136:154	A thermostable β-1,3-galactosidase	121:154	A thermostable β-1,3-galactosidase from Marinomonas sp	121:174	A thermostable β-1,3-galactosidase from Marinomonas sp.					
30380738	8	23	theme	galactooligosaccharides	1345:1367	arg1	synthesis					1332:1340	synthesis	1332:1340	synthesis of galactooligosaccharides (GOS)	1332:1373	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	0	24	theme	Heterologous	0:11	arg1	Expression					13:22	Heterologous Expression	0:22	Heterologous Expression of a Thermostable β-1,3-Galactosidase	0:60	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	3	25	theme	electrophoretic	559:573	arg1	purity					575:580	electrophoretic purity	559:580	electrophoretic purity	559:580	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	9	26	theme	β-1,4	1637:1641	arg1	linkage					1643:1649	β-1,4 linkage	1637:1649	β-1,4 linkage	1637:1649	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	7	27	theme	enzyme	1129:1134	arg1	stability					1112:1120	the stability	1108:1120	the stability of the enzyme	1108:1134	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	4	28	from	°C	730:731	arg1	mg-1					719:722	126.4 U mg-1	711:722	126.4 U mg-1	711:722	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	4	28	from	°C	730:731	arg1	activity					675:682	The specific activity	662:682	The specific activity of the purified enzyme	662:705	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	5	29	theme	type	927:930	arg1	MaBGA					936:940	wild type (wt)MaBGA	922:940	wild type (wt)MaBGA	922:940	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	29	theme	type	927:930	arg1	counterpart					909:919	its wild-type counterpart	895:919	its wild-type counterpart	895:919	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	2	30	theme	optimum	265:271	arg1	conditions					289:298	optimum over-expression conditions	265:298	optimum over-expression conditions as follows	265:309	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	5	31	theme	rMaBGA	824:829	arg1	°C					853:854	60 °C	850:854	60 °C	850:854	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	31	theme	rMaBGA	824:829	arg1	pH					818:819	pH	818:819	pH of rMaBGA	818:829	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	31	theme	rMaBGA	824:829	arg1	temperature					802:812	The optimum temperature	790:812	The optimum temperature	790:812	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	31	theme	rMaBGA	824:829	arg1	6.0					860:862	6.0	860:862	6.0	860:862	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	32	theme	wild	922:925	arg1	type					927:930	wild type	922:930	wild type (wt)MaBGA	922:940	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	32	theme	wild	922:925	arg1	wt					933:934	wt	933:934	wt	933:934	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	10	33	theme	promising	1732:1740	arg1	alternative					1742:1752	a promising alternative	1730:1752	a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage	1730:1802	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	10	33	theme	promising	1732:1740	arg1	enzyme					1714:1719	the recombinant enzyme	1698:1719	the recombinant enzyme	1698:1719	The results indicated that the recombinant enzyme would be a promising alternative for biosynthesis of GOS mainly with β-1,3 linkage.					
30380738	2	34	theme	Escherichia	231:241	arg1	BL21					248:251	Escherichia coli BL21	231:251	Escherichia coli BL21 (DE3)	231:257	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	2	34	theme	Escherichia	231:241	arg1	DE3					254:256	DE3	254:256	DE3	254:256	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	1	35	theme	thermostable	123:134	arg1	β-1,3-galactosidase					136:154	A thermostable β-1,3-galactosidase	121:154	A thermostable β-1,3-galactosidase from Marinomonas sp	121:174	A thermostable β-1,3-galactosidase from Marinomonas sp.					
30380738	4	36	theme	enzyme	700:705	arg1	activity					675:682	The specific activity	662:682	The specific activity of the purified enzyme	662:705	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	4	36	theme	enzyme	700:705	arg1	mg-1					719:722	126.4 U mg-1	711:722	126.4 U mg-1	711:722	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	0	37	theme	β-1,3-Galactosidase	42:60	arg1	Potential					70:78	Its Potential	66:78	Its Potential in Synthesis of Galactooligosaccharides	66:118	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	0	37	theme	β-1,3-Galactosidase	42:60	arg1	Expression					13:22	Heterologous Expression	0:22	Heterologous Expression of a Thermostable β-1,3-Galactosidase	0:60	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	7	38	theme	steady-state	1266:1277	arg1	kinetics					1279:1286	the steady-state kinetics	1262:1286	the steady-state kinetics	1262:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	2	39	dep	reached	416:422	arg1	followed					445:452	followed	445:452	followed by 22 h incubation at 20 °C	445:480	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	3	40	theme	immobilized	585:595	arg1	chromatography					612:625	immobilized metal affinity chromatography	585:625	immobilized metal affinity chromatography	585:625	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	2	41	theme	h	460:460	arg1	incubation					462:471	22 h incubation	457:471	22 h incubation at 20 °C	457:480	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	0	42	theme	Thermostable	29:40	arg1	β-1,3-Galactosidase					42:60	a Thermostable β-1,3-Galactosidase	27:60	a Thermostable β-1,3-Galactosidase	27:60	Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides.					
30380738	3	43	theme	exclusion	636:644	arg1	chromatography					646:659	size exclusion chromatography	631:659	size exclusion chromatography	631:659	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	3	44	theme	metal	597:601	arg1	chromatography					612:625	immobilized metal affinity chromatography	585:625	immobilized metal affinity chromatography	585:625	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	2	45	theme	culture	408:414	arg1	OD600					395:399	the OD600	391:399	the OD600 of the culture	391:414	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	2	46	theme	22	457:458	arg1	h					460:460	h	460:460	h	460:460	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	2	47	from	°C	479:480	arg1	incubation					462:471	22 h incubation	457:471	22 h incubation at 20 °C	457:480	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	8	48	theme	purified	1380:1387	arg1	enzyme					1401:1406	the purified recombinant enzyme	1376:1406	the purified recombinant enzyme	1376:1406	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	2	49	theme	IPTG	367:370	arg1	mM					361:362	0.1 mM	357:362	0.1 mM of IPTG	357:370	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	5	50	theme	optimum	794:800	arg1	°C					853:854	60 °C	850:854	60 °C	850:854	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	50	theme	optimum	794:800	arg1	pH					818:819	pH	818:819	pH of rMaBGA	818:829	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	50	theme	optimum	794:800	arg1	temperature					802:812	The optimum temperature	790:812	The optimum temperature	790:812	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	50	theme	optimum	794:800	arg1	6.0					860:862	6.0	860:862	6.0	860:862	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	9	51	theme	better	1595:1600	arg1	3'-galactosyl-lactose					1542:1562	3'-galactosyl-lactose	1542:1562	3'-galactosyl-lactose	1542:1562	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	9	51	theme	better	1595:1600	arg1	effector					1614:1621	a better bifidogenic effector	1593:1621	a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages	1593:1668	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	3	52	theme	recombinant	487:497	arg1	rMaBGA					527:532	rMaBGA	527:532	rMaBGA	527:532	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	3	52	theme	recombinant	487:497	arg1	β-1,3-galactosidase					506:524	The recombinant enzyme β-1,3-galactosidase	483:524	The recombinant enzyme β-1,3-galactosidase (rMaBGA)	483:533	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	4	53	theme	U	717:717	arg1	mg-1					719:722	126.4 U mg-1	711:722	126.4 U mg-1	711:722	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	4	53	theme	U	717:717	arg1	activity					675:682	The specific activity	662:682	The specific activity of the purified enzyme	662:705	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30380738	9	54	theme	bifidogenic	1602:1612	arg1	3'-galactosyl-lactose					1542:1562	3'-galactosyl-lactose	1542:1562	3'-galactosyl-lactose	1542:1562	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	9	54	theme	bifidogenic	1602:1612	arg1	effector					1614:1621	a better bifidogenic effector	1593:1621	a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages	1593:1668	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	7	55	with	expression	1164:1173	arg1	absence					1184:1190	the absence	1180:1190	the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics	1180:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	1	56	theme	Marinomonas	161:171	arg1	sp					173:174	Marinomonas sp	161:174	Marinomonas sp	161:174	A thermostable β-1,3-galactosidase from Marinomonas sp.					
30380738	6	57	theme	thermal	991:997	arg1	stability					999:1007	different thermal stability	981:1007	different thermal stability	981:1007	However, rMaBGA and wtMaBGA displayed different thermal stability and steady-state kinetics, although they share identical primary structures.					
30380738	8	58	theme	lab	1474:1476	arg1	scale					1478:1482	the lab scale	1470:1482	the lab scale	1470:1482	To evaluate the potential of the enzyme in synthesis of galactooligosaccharides (GOS), the purified recombinant enzyme was employed to catalyze the transgalactosylation reaction at the lab scale.					
30380738	3	59	theme	size	631:634	arg1	chromatography					646:659	size exclusion chromatography	631:659	size exclusion chromatography	631:659	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	9	60	theme	transgalactosylation	1496:1515	arg1	products					1517:1524	the transgalactosylation products	1492:1524	the transgalactosylation products	1492:1524	One of the transgalactosylation products was resolved as 3'-galactosyl-lactose, which had been proven to be a better bifidogenic effector than GOS with β-1,4 linkage and β-1,6 linkages.					
30380738	5	61	theme	wild-type	899:907	arg1	MaBGA					936:940	wild type (wt)MaBGA	922:940	wild type (wt)MaBGA	922:940	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	5	61	theme	wild-type	899:907	arg1	counterpart					909:919	its wild-type counterpart	895:919	its wild-type counterpart	895:919	The optimum temperature and pH of rMaBGA were determined as 60 °C and 6.0, respectively, resembling with its wild-type counterpart, wild type (wt)MaBGA.					
30380738	7	62	theme	post-translational	1195:1212	arg1	glycosylation					1236:1248	glycosylation	1236:1248	glycosylation	1236:1248	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	7	62	theme	post-translational	1195:1212	arg1	kinetics					1279:1286	the steady-state kinetics	1262:1286	the steady-state kinetics	1262:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	7	62	theme	post-translational	1195:1212	arg1	modifications					1214:1226	post-translational modifications	1195:1226	post-translational modifications such as glycosylation, as well as the steady-state kinetics	1195:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	3	63	theme	affinity	603:610	arg1	chromatography					612:625	immobilized metal affinity chromatography	585:625	immobilized metal affinity chromatography	585:625	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	3	64	theme	enzyme	499:504	arg1	rMaBGA					527:532	rMaBGA	527:532	rMaBGA	527:532	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	3	64	theme	enzyme	499:504	arg1	β-1,3-galactosidase					506:524	The recombinant enzyme β-1,3-galactosidase	483:524	The recombinant enzyme β-1,3-galactosidase (rMaBGA)	483:533	The recombinant enzyme β-1,3-galactosidase (rMaBGA) was further purified to electrophoretic purity by immobilized metal affinity chromatography and size exclusion chromatography.					
30380738	7	65	theme	modifications	1214:1226	arg1	absence					1184:1190	the absence	1180:1190	the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics	1180:1286	It is postulated that the stability of the enzyme was altered by heterologous expression with the absence of post-translational modifications such as glycosylation, as well as the steady-state kinetics.					
30380738	2	66	theme	over-expression	273:287	arg1	conditions					289:298	optimum over-expression conditions	265:298	optimum over-expression conditions as follows	265:309	BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD600 of the culture reached between 0.6 and 0.9, followed by 22 h incubation at 20 °C.					
30380738	4	67	theme	purified	691:698	arg1	enzyme					700:705	the purified enzyme	687:705	the purified enzyme	687:705	The specific activity of the purified enzyme was 126.4 U mg-1 at 37 °C using ONPG (o-nitrophenyl-β-galactoside) as a substrate.					
30669480	7	0	theme	immunostimulatory	1117:1133	arg1	activity					1135:1142	the greatest immunostimulatory activity	1104:1142	the greatest immunostimulatory activity	1104:1142	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	3	1	theme	polysaccharides	543:557	arg1	weights					528:534	the molecular weights	514:534	the molecular weights of the polysaccharides	514:557	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	4	2	theme	persimmon	637:645	arg1	variation					624:632	seasonal variation	615:632	seasonal variation of persimmon leaves	615:652	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	1	3	from	changes	158:164	arg1	features					202:209	the structural and immunological features	169:209	the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages	169:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	6	4	theme	immunostimulatory	939:955	arg1	effects					957:963	their immunostimulatory effects	933:963	their immunostimulatory effects on RAW264.7 macrophages	933:987	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	9	5	theme	persimmon	1541:1549	arg1	strategies					1568:1577	persimmon leaf cultivation strategies	1541:1577	persimmon leaf cultivation strategies for food and medical uses of the polysaccharides	1541:1626	The results also provide a basis for optimizing persimmon leaf cultivation strategies for food and medical uses of the polysaccharides.					
30669480	0	6	theme	Maturity	110:117	arg1	Stages					119:124	Different Maturity Stages	100:124	Different Maturity Stages	100:124	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	1	7	dep	persimmon	277:285	arg1	leaves					287:292	leaves	287:292	leaves at three different growth stages	287:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	9	8	theme	leaf	1551:1554	arg1	strategies					1568:1577	persimmon leaf cultivation strategies	1541:1577	persimmon leaf cultivation strategies for food and medical uses of the polysaccharides	1541:1626	The results also provide a basis for optimizing persimmon leaf cultivation strategies for food and medical uses of the polysaccharides.					
30669480	0	9	theme	Different	100:108	arg1	Stages					119:124	Different Maturity Stages	100:124	Different Maturity Stages	100:124	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	7	10	from	upregulation	1249:1260	arg1	macrophages					1290:1300	macrophages	1290:1300	macrophages	1290:1300	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	8	11	theme	immunostimulatory	1463:1479	arg1	properties					1481:1490	their immunostimulatory properties	1457:1490	their immunostimulatory properties	1457:1490	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	9	12	theme	cultivation	1556:1566	arg1	strategies					1568:1577	persimmon leaf cultivation strategies	1541:1577	persimmon leaf cultivation strategies for food and medical uses of the polysaccharides	1541:1626	The results also provide a basis for optimizing persimmon leaf cultivation strategies for food and medical uses of the polysaccharides.					
30669480	6	13	theme	>	1067:1067	arg1	activities					1018:1027	their activities	1012:1027	their activities	1012:1027	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	6	13	theme	>	1067:1067	arg1	S1-PLE0					1069:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	7	14	theme	expression	1276:1285	arg1	upregulation					1249:1260	the upregulation	1245:1260	the upregulation of their gene expression in macrophages	1245:1300	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	7	15	theme	nitric	1195:1200	arg1	NO					1209:1210	NO	1209:1210	NO	1209:1210	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	7	15	theme	nitric	1195:1200	arg1	oxide					1202:1206	nitric oxide	1195:1206	nitric oxide (NO)	1195:1211	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	4	16	theme	glycosidic	690:699	arg1	linkages					701:708	glycosidic linkages	690:708	glycosidic linkages	690:708	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	3	17	theme	growth	587:592	arg1	stages					594:599	successive growth stages	576:599	successive growth stages	576:599	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	8	18	theme	structural	1349:1358	arg1	features					1360:1367	the structural features	1345:1367	the structural features of polysaccharides according to the different maturity of persimmon leaves	1345:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	3	19	theme	High-performance	456:471	arg1	chromatograms					488:500	High-performance size-exclusion chromatograms	456:500	High-performance size-exclusion chromatograms	456:500	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	7	20	theme	cytokines	1217:1225	arg1	secretion					1227:1235	nitric oxide (NO) and cytokines secretion	1195:1235	nitric oxide (NO) and cytokines secretion	1195:1235	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	0	21	from	Features	47:54	arg1	Persimmon					80:88	Persimmon	80:88	Persimmon	80:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	7	22	theme	oxide	1202:1206	arg1	secretion					1227:1235	nitric oxide (NO) and cytokines secretion	1195:1235	nitric oxide (NO) and cytokines secretion	1195:1235	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	7	23	theme	gene	1271:1274	arg1	expression					1276:1285	their gene expression	1265:1285	their gene expression in macrophages	1265:1300	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	2	24	theme	weight	406:411	arg1	distributions					413:425	molecular weight distributions	396:425	molecular weight distributions	396:425	Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed.					
30669480	5	25	theme	β-glucopyranoside	864:880	arg1	linkages					882:889	β-glucopyranoside linkages	864:889	β-glucopyranoside linkages	864:889	S2-PLE0 was composed of comparatively more galactose, arabinose, rhamnose, xylose, and galacturonic acid, showing the presence of β-glucopyranoside linkages.					
30669480	6	26	theme	RAW264.7	968:975	arg1	macrophages					977:987	RAW264.7 macrophages	968:987	RAW264.7 macrophages	968:987	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	2	27	theme	molecular	396:404	arg1	distributions					413:425	molecular weight distributions	396:425	molecular weight distributions	396:425	Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed.					
30669480	5	28	theme	linkages	882:889	arg1	presence					852:859	the presence	848:859	the presence of β-glucopyranoside linkages	848:889	S2-PLE0 was composed of comparatively more galactose, arabinose, rhamnose, xylose, and galacturonic acid, showing the presence of β-glucopyranoside linkages.					
30669480	6	29	theme	S3-PLE0	1059:1065	arg1	activities					1018:1027	their activities	1012:1027	their activities	1012:1027	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	6	29	theme	S3-PLE0	1059:1065	arg1	S1-PLE0					1069:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	1	30	theme	immunological	188:200	arg1	features					202:209	the structural and immunological features	169:209	the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages	169:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	6	31	theme	>	1057:1057	arg1	activities					1018:1027	their activities	1012:1027	their activities	1012:1027	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	6	31	theme	>	1057:1057	arg1	S1-PLE0					1069:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	7	32	theme	phagocytic	1161:1170	arg1	capacity					1172:1179	the phagocytic capacity	1157:1179	the phagocytic capacity	1157:1179	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	6	33	theme	Significant	892:902	arg1	differences					904:914	Significant differences	892:914	Significant differences	892:914	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	0	34	theme	Structural	18:27	arg1	Features					47:54	Structural and Immunological Features	18:54	Structural and Immunological Features of Polysaccharides from Persimmon	18:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	6	35	theme	S2-PLE0	1049:1055	arg1	activities					1018:1027	their activities	1012:1027	their activities	1012:1027	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	6	35	theme	S2-PLE0	1049:1055	arg1	S1-PLE0					1069:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	S2-PLE0 > S3-PLE0 > S1-PLE0	1049:1075	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	7	36	from	macrophages	1290:1300	arg1	upregulation					1249:1260	the upregulation	1245:1260	the upregulation of their gene expression in macrophages	1245:1300	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	7	37	from	expression	1276:1285	arg1	macrophages					1290:1300	macrophages	1290:1300	macrophages	1290:1300	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	4	38	from	linkages	701:708	arg1	polysaccharides					717:731	the polysaccharides	713:731	the polysaccharides	713:731	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	2	39	theme	linkage	432:438	arg1	types					440:444	linkage types	432:444	linkage types	432:444	Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed.					
30669480	0	40	theme	Features	47:54	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon	0:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	4	41	theme	seasonal	615:622	arg1	variation					624:632	seasonal variation	615:632	seasonal variation of persimmon leaves	615:652	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	9	42	dep	food	1583:1586	arg1	uses					1600:1603	uses	1600:1603	uses	1600:1603	The results also provide a basis for optimizing persimmon leaf cultivation strategies for food and medical uses of the polysaccharides.					
30669480	1	43	theme	polysaccharides	214:228	arg1	features					202:209	the structural and immunological features	169:209	the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages	169:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	0	44	theme	Immunological	33:45	arg1	Features					47:54	Structural and Immunological Features	18:54	Structural and Immunological Features of Polysaccharides from Persimmon	18:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	0	45	from	Persimmon	80:88	arg1	Features					47:54	Structural and Immunological Features	18:54	Structural and Immunological Features of Polysaccharides from Persimmon	18:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	0	45	from	Persimmon	80:88	arg1	Polysaccharides					59:73	Polysaccharides	59:73	Polysaccharides from Persimmon	59:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	8	46	theme	different	1405:1413	arg1	maturity					1415:1422	the different maturity	1401:1422	the different maturity of persimmon leaves	1401:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	8	47	theme	polysaccharides	1372:1386	arg1	features					1360:1367	the structural features	1345:1367	the structural features of polysaccharides according to the different maturity of persimmon leaves	1345:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	0	48	theme	Polysaccharides	59:73	arg1	Features					47:54	Structural and Immunological Features	18:54	Structural and Immunological Features of Polysaccharides from Persimmon	18:88	Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages.					
30669480	1	49	theme	different	303:311	arg1	stages					320:325	three different growth stages	297:325	three different growth stages	297:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	5	50	theme	galacturonic	821:832	arg1	acid					834:837	galacturonic acid	821:837	galacturonic acid	821:837	S2-PLE0 was composed of comparatively more galactose, arabinose, rhamnose, xylose, and galacturonic acid, showing the presence of β-glucopyranoside linkages.					
30669480	8	51	dep	persimmon	1427:1435	arg1	leaves					1437:1442	leaves	1437:1442	leaves	1437:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	1	52	theme	growth	313:318	arg1	stages					320:325	three different growth stages	297:325	three different growth stages	297:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	3	53	theme	molecular	518:526	arg1	weights					528:534	the molecular weights	514:534	the molecular weights of the polysaccharides	514:557	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	4	54	dep	persimmon	637:645	arg1	leaves					647:652	leaves	647:652	leaves	647:652	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	7	55	theme	greatest	1108:1115	arg1	activity					1135:1142	the greatest immunostimulatory activity	1104:1142	the greatest immunostimulatory activity	1104:1142	Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages.					
30669480	1	56	dep	polysaccharides	214:228	arg1	S2-PLE0					240:246	S2-PLE0	240:246	S2-PLE0	240:246	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	1	56	dep	polysaccharides	214:228	arg1	S3-PLE0					253:259	S3-PLE0	253:259	S3-PLE0	253:259	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	1	56	dep	polysaccharides	214:228	arg1	S1-PLE0					231:237	S1-PLE0	231:237	S1-PLE0	231:237	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	1	56	dep	polysaccharides	214:228	arg1	polysaccharides					214:228	polysaccharides	214:228	polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages	214:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	8	57	from	differences	1330:1340	arg1	features					1360:1367	the structural features	1345:1367	the structural features of polysaccharides according to the different maturity of persimmon leaves	1345:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	2	58	theme	Physicochemical	328:342	arg1	analyses					344:351	Physicochemical analyses	328:351	Physicochemical analyses	328:351	Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed.					
30669480	4	59	theme	sugar	667:671	arg1	compositions					673:684	the sugar compositions	663:684	the sugar compositions	663:684	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	5	60	theme	more	772:775	arg1	galactose					777:785	comparatively more galactose	758:785	comparatively more galactose	758:785	S2-PLE0 was composed of comparatively more galactose, arabinose, rhamnose, xylose, and galacturonic acid, showing the presence of β-glucopyranoside linkages.					
30669480	3	61	theme	successive	576:585	arg1	stages					594:599	successive growth stages	576:599	successive growth stages	576:599	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	3	62	theme	size-exclusion	473:486	arg1	chromatograms					488:500	High-performance size-exclusion chromatograms	456:500	High-performance size-exclusion chromatograms	456:500	High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages.					
30669480	8	63	theme	persimmon	1427:1435	arg1	maturity					1415:1422	the different maturity	1401:1422	the different maturity of persimmon leaves	1401:1442	These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties.					
30669480	1	64	theme	structural	173:182	arg1	features					202:209	the structural and immunological features	169:209	the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages	169:325	In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages.					
30669480	4	65	from	compositions	673:684	arg1	polysaccharides					717:731	the polysaccharides	713:731	the polysaccharides	713:731	In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides.					
30669480	6	66	from	effects	957:963	arg1	macrophages					977:987	RAW264.7 macrophages	968:987	RAW264.7 macrophages	968:987	Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0.					
30669480	2	67	theme	chemical	373:380	arg1	compositions					382:393	their chemical compositions	367:393	their chemical compositions	367:393	Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed.					
30959133	0	0	theme	longan	93:98	arg1	pulp					100:103	longan pulp	93:103	longan pulp	93:103	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	7	1	theme	fermented	1035:1043	arg1	LPs					1045:1047	fermented LPs	1035:1047	fermented LPs	1035:1047	When compared with unfermented LP-0, the glucose molar percentages of fermented LPs declined, while those of rhamnose and galactose increased, except for LP-6.					
30959133	9	2	theme	prebiotic	1413:1421	arg1	activities					1423:1432	the prebiotic activities	1409:1432	the prebiotic activities of polysaccharides from longan pulp	1409:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	8	3	theme	Fermented	1125:1133	arg1	LPs					1135:1137	Fermented LPs	1125:1137	Fermented LPs	1125:1137	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	0	4	from	variation	8:16	arg1	properties					33:42	biochemical properties	21:42	biochemical properties	21:42	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	0	4	from	variation	8:16	arg1	activities					58:67	prebiotic activities	48:67	prebiotic activities	48:67	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	1	5	theme	important	174:182	arg1	technology					195:204	an important processing technology	171:204	an important processing technology for fruits and vegetables	171:230	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	1	5	theme	important	174:182	arg1	fermentation					155:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	3	6	theme	fermentation	392:403	arg1	times					405:409	different fermentation times	382:409	different fermentation times	382:409	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	4	7	theme	different	763:771	arg1	times					786:790	different fermentation times	763:790	different fermentation times	763:790	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	9	8	from	pulp	1465:1468	arg1	activities					1423:1432	the prebiotic activities	1409:1432	the prebiotic activities of polysaccharides from longan pulp	1409:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	9	8	from	pulp	1465:1468	arg1	polysaccharides					1437:1451	polysaccharides	1437:1451	polysaccharides from longan pulp	1437:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	0	9	from	activities	58:67	arg1	pulp					100:103	longan pulp	93:103	longan pulp	93:103	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	9	10	theme	polysaccharides	1437:1451	arg1	activities					1423:1432	the prebiotic activities	1409:1432	the prebiotic activities of polysaccharides from longan pulp	1409:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	8	11	theme	stronger	1156:1163	arg1	effect					1177:1182	a stronger stimulatory effect	1154:1182	a stronger stimulatory effect on Lactobacillus strain proliferation	1154:1220	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	6	12	theme	smallest	903:910	arg1	108.71 ± 5.55 kDa					916:932	108.71 ± 5.55 kDa	916:932	108.71 ± 5.55 kDa	916:932	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	6	12	theme	smallest	903:910	arg1	Mw					912:913	the smallest Mw	899:913	the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05)	899:962	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	8	13	theme	LP-12	1256:1260	arg1	effect					1246:1251	the proliferative effect	1228:1251	the proliferative effect of LP-12	1228:1260	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	8	13	theme	LP-12	1256:1260	arg1	strongest					1272:1280	strongest	1272:1280	strongest	1272:1280	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	1	14	theme	processing	184:193	arg1	technology					195:204	an important processing technology	171:204	an important processing technology for fruits and vegetables	171:230	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	1	14	theme	processing	184:193	arg1	fermentation					155:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	0	15	theme	fermentation	112:123	arg1	process					125:131	fermentation process	112:131	fermentation process	112:131	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	2	16	theme	fermentation	300:311	arg1	process					313:319	the fermentation process	296:319	the fermentation process	296:319	Bioactive compounds such as polysaccharides are altered during the fermentation process.					
30959133	3	17	theme	longan	489:494	arg1	polysaccharides					496:510	longan polysaccharides	489:510	longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively	489:613	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	3	18	theme	physicochemical	421:435	arg1	properties					451:460	their physicochemical and prebiotic properties	415:460	their physicochemical and prebiotic properties	415:460	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	0	19	from	properties	33:42	arg1	pulp					100:103	longan pulp	93:103	longan pulp	93:103	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	9	20	theme	lactic	1321:1326	arg1	bacteria					1333:1340	lactic acid bacteria fermentation	1321:1353	lactic acid bacteria fermentation	1321:1353	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	0	21	theme	Dynamic	0:6	arg1	variation					8:16	Dynamic variation	0:16	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.	0:132	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	8	22	from	effect	1177:1182	arg1	proliferation					1208:1220	Lactobacillus strain proliferation	1187:1220	Lactobacillus strain proliferation	1187:1220	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	8	23	theme	proliferative	1232:1244	arg1	effect					1246:1251	the proliferative effect	1228:1251	the proliferative effect of LP-12	1228:1260	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	8	23	theme	proliferative	1232:1244	arg1	strongest					1272:1280	strongest	1272:1280	strongest	1272:1280	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	0	24	theme	biochemical	21:31	arg1	properties					33:42	biochemical properties	21:42	biochemical properties	21:42	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	9	25	theme	acid	1328:1331	arg1	bacteria					1333:1340	lactic acid bacteria fermentation	1321:1353	lactic acid bacteria fermentation	1321:1353	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	4	26	theme	fermentation	773:784	arg1	times					786:790	different fermentation times	763:790	different fermentation times	763:790	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	27	theme	acid	664:667	arg1	contents					627:634	contents	627:634	contents of neutral sugar and uronic acid	627:667	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	27	theme	acid	664:667	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition of LPs	697:729	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	27	theme	acid	664:667	arg1	Mw					688:689	Mw	688:689	Mw	688:689	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	27	theme	acid	664:667	arg1	yield					620:624	The yield	616:624	The yield	616:624	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	27	theme	acid	664:667	arg1	weight					680:685	molecular weight	670:685	molecular weight (Mw)	670:690	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	5	28	theme	LPs	841:843	arg1	content					830:836	uronic acid content	818:836	uronic acid content	818:836	Specially, the yield and uronic acid content of LPs were first increase and then decline.					
30959133	5	28	theme	LPs	841:843	arg1	yield					808:812	the yield	804:812	the yield	804:812	Specially, the yield and uronic acid content of LPs were first increase and then decline.					
30959133	4	29	theme	monosaccharide	697:710	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition of LPs	697:729	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	30	theme	uronic	657:662	arg1	acid					664:667	uronic acid	657:667	uronic acid	657:667	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	31	theme	molecular	670:678	arg1	Mw					688:689	Mw	688:689	Mw	688:689	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	31	theme	molecular	670:678	arg1	weight					680:685	molecular weight	670:685	molecular weight (Mw)	670:690	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	8	32	theme	strain	1201:1206	arg1	proliferation					1208:1220	Lactobacillus strain proliferation	1187:1220	Lactobacillus strain proliferation	1187:1220	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	9	33	from	activities	1423:1432	arg1	pulp					1465:1468	longan pulp	1458:1468	longan pulp	1458:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	8	34	theme	Lactobacillus	1187:1199	arg1	proliferation					1208:1220	Lactobacillus strain proliferation	1187:1220	Lactobacillus strain proliferation	1187:1220	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	7	35	theme	glucose	1006:1012	arg1	percentages					1020:1030	the glucose molar percentages	1002:1030	the glucose molar percentages of fermented LPs	1002:1047	When compared with unfermented LP-0, the glucose molar percentages of fermented LPs declined, while those of rhamnose and galactose increased, except for LP-6.					
30959133	3	36	theme	different	382:390	arg1	times					405:409	different fermentation times	382:409	different fermentation times	382:409	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	3	37	theme	prebiotic	441:449	arg1	properties					451:460	their physicochemical and prebiotic properties	415:460	their physicochemical and prebiotic properties	415:460	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	6	38	theme	LPs	949:951	arg1	108.71 ± 5.55 kDa					916:932	108.71 ± 5.55 kDa	916:932	108.71 ± 5.55 kDa	916:932	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	6	38	theme	LPs	949:951	arg1	Mw					912:913	the smallest Mw	899:913	the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05)	899:962	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	5	39	theme	uronic	818:823	arg1	content					830:836	uronic acid content	818:836	uronic acid content	818:836	Specially, the yield and uronic acid content of LPs were first increase and then decline.					
30959133	7	40	theme	molar	1014:1018	arg1	percentages					1020:1030	the glucose molar percentages	1002:1030	the glucose molar percentages of fermented LPs	1002:1047	When compared with unfermented LP-0, the glucose molar percentages of fermented LPs declined, while those of rhamnose and galactose increased, except for LP-6.					
30959133	4	41	theme	LPs	727:729	arg1	contents					627:634	contents	627:634	contents of neutral sugar and uronic acid	627:667	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	41	theme	LPs	727:729	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition of LPs	697:729	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	41	theme	LPs	727:729	arg1	Mw					688:689	Mw	688:689	Mw	688:689	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	41	theme	LPs	727:729	arg1	yield					620:624	The yield	616:624	The yield	616:624	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	41	theme	LPs	727:729	arg1	weight					680:685	molecular weight	670:685	molecular weight (Mw)	670:690	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	0	42	theme	prebiotic	48:56	arg1	activities					58:67	prebiotic activities	48:67	prebiotic activities	48:67	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	6	43	theme	tested	942:947	arg1	LPs					949:951	the tested LPs	938:951	the tested LPs (p < 0.05)	938:962	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	6	43	theme	tested	942:947	arg1	p < 0.05					954:961	p < 0.05	954:961	p < 0.05	954:961	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	7	44	theme	LPs	1045:1047	arg1	percentages					1020:1030	the glucose molar percentages	1002:1030	the glucose molar percentages of fermented LPs	1002:1047	When compared with unfermented LP-0, the glucose molar percentages of fermented LPs declined, while those of rhamnose and galactose increased, except for LP-6.					
30959133	2	45	theme	Bioactive	233:241	arg1	compounds					243:251	Bioactive compounds	233:251	Bioactive compounds such as polysaccharides	233:275	Bioactive compounds such as polysaccharides are altered during the fermentation process.					
30959133	2	45	theme	Bioactive	233:241	arg1	polysaccharides					261:275	polysaccharides	261:275	polysaccharides	261:275	Bioactive compounds such as polysaccharides are altered during the fermentation process.					
30959133	1	46	theme	Lactic	134:139	arg1	bacteria					146:153	Lactic acid bacteria	134:153	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	9	47	theme	physicochemical	1370:1384	arg1	properties					1386:1395	the physicochemical properties	1366:1395	the physicochemical properties	1366:1395	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	1	48	theme	acid	141:144	arg1	bacteria					146:153	Lactic acid bacteria	134:153	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	4	49	theme	neutral	639:645	arg1	sugar					647:651	neutral sugar	639:651	neutral sugar	639:651	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	3	50	theme	longan	343:348	arg1	LPs					356:358	LPs	356:358	LPs	356:358	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	3	50	theme	longan	343:348	arg1	pulp					350:353	longan pulp	343:353	longan pulp (LPs)	343:359	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	8	51	theme	stimulatory	1165:1175	arg1	effect					1177:1182	a stronger stimulatory effect	1154:1182	a stronger stimulatory effect on Lactobacillus strain proliferation	1154:1220	Fermented LPs also exhibited a stronger stimulatory effect on Lactobacillus strain proliferation, with the proliferative effect of LP-12 being the strongest (p < 0.05).					
30959133	7	52	theme	unfermented	984:994	arg1	LP-0					996:999	unfermented LP-0	984:999	unfermented LP-0	984:999	When compared with unfermented LP-0, the glucose molar percentages of fermented LPs declined, while those of rhamnose and galactose increased, except for LP-6.					
30959133	1	53	theme	bacteria	146:153	arg1	technology					195:204	an important processing technology	171:204	an important processing technology for fruits and vegetables	171:230	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	1	53	theme	bacteria	146:153	arg1	fermentation					155:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation	134:166	Lactic acid bacteria fermentation is an important processing technology for fruits and vegetables.					
30959133	0	54	theme	polysaccharides	72:86	arg1	properties					33:42	biochemical properties	21:42	biochemical properties	21:42	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	0	54	theme	polysaccharides	72:86	arg1	activities					58:67	prebiotic activities	48:67	prebiotic activities	48:67	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	0	55	from	pulp	100:103	arg1	polysaccharides					72:86	polysaccharides	72:86	polysaccharides from longan pulp	72:103	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	0	55	from	pulp	100:103	arg1	properties					33:42	biochemical properties	21:42	biochemical properties	21:42	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	0	55	from	pulp	100:103	arg1	activities					58:67	prebiotic activities	48:67	prebiotic activities	48:67	Dynamic variation in biochemical properties and prebiotic activities of polysaccharides from longan pulp during fermentation process.					
30959133	3	56	dep	LP-0	518:521	arg1	extracted					549:557	extracted	549:557	were extracted from longan pulp fermented for 0 and 12 h, respectively	544:613	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	6	57	contain	contained	889:897	arg2	108.71 ± 5.55 kDa					916:932	108.71 ± 5.55 kDa	916:932	108.71 ± 5.55 kDa	916:932	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	6	57	contain	contained	889:897	arg2	Mw					912:913	the smallest Mw	899:913	the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05)	899:962	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	6	57	contain	contained	889:897	arg1	LP-12					883:887	LP-12	883:887	LP-12	883:887	LP-12 contained the smallest Mw (108.71 ± 5.55 kDa) of the tested LPs (p < 0.05).					
30959133	3	58	theme	longan	564:569	arg1	pulp					571:574	longan pulp	564:574	longan pulp fermented for 0 and 12 h, respectively	564:613	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	9	59	theme	longan	1458:1463	arg1	pulp					1465:1468	longan pulp	1458:1468	longan pulp	1458:1468	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	3	60	from	pulp	350:353	arg1	Polysaccharides					322:336	Polysaccharides	322:336	Polysaccharides from longan pulp (LPs)	322:359	Polysaccharides from longan pulp (LPs) were extracted after different fermentation times and their physicochemical and prebiotic properties were investigated, such as longan polysaccharides named LP-0 and LP-12 means they were extracted from longan pulp fermented for 0 and 12 h, respectively.					
30959133	9	61	dep	bacteria	1333:1340	arg1	fermentation					1342:1353	fermentation	1342:1353	lactic acid bacteria fermentation	1321:1353	These results suggest that lactic acid bacteria fermentation can change the physicochemical properties and enhance the prebiotic activities of polysaccharides from longan pulp.					
30959133	5	62	theme	acid	825:828	arg1	content					830:836	uronic acid content	818:836	uronic acid content	818:836	Specially, the yield and uronic acid content of LPs were first increase and then decline.					
30959133	4	63	theme	sugar	647:651	arg1	contents					627:634	contents	627:634	contents of neutral sugar and uronic acid	627:667	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	63	theme	sugar	647:651	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition of LPs	697:729	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	63	theme	sugar	647:651	arg1	Mw					688:689	Mw	688:689	Mw	688:689	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	63	theme	sugar	647:651	arg1	yield					620:624	The yield	616:624	The yield	616:624	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
30959133	4	63	theme	sugar	647:651	arg1	weight					680:685	molecular weight	670:685	molecular weight (Mw)	670:690	The yield, contents of neutral sugar and uronic acid, molecular weight (Mw), and monosaccharide composition of LPs were significantly changed with different fermentation times.					
31320077	5	0	from	α1→3	891:894	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	0	from	α1→3	891:894	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	7	1	theme	N.	1067:1068	arg1	polysaccharides					1092:1106	the N. oculata water-soluble polysaccharides	1063:1106	the N. oculata water-soluble polysaccharides	1063:1106	Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.					
31320077	4	2	theme	carbohydrate	667:678	arg1	microarrays					680:690	carbohydrate microarrays	667:690	carbohydrate microarrays	667:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	5	3	from	-glucans	880:887	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	3	from	-glucans	880:887	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	4	from	oculata	847:853	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	4	from	oculata	847:853	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	4	5	theme	sugar	575:579	arg1	analysis					597:604	methylation analysis	585:604	methylation analysis	585:604	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	4	5	theme	sugar	575:579	arg1	combination					560:570	A combination	558:570	A combination of sugar	558:579	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	2	6	theme	microalgae	324:333	arg1	polysaccharides					335:349	microalgae polysaccharides	324:349	microalgae polysaccharides	324:349	Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce.					
31320077	2	7	theme	polysaccharides	335:349	arg1	structure					311:319	structure	311:319	structure	311:319	Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce.					
31320077	2	7	theme	polysaccharides	335:349	arg1	composition					295:305	composition	295:305	composition	295:305	Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce.					
31320077	4	8	with	combination	560:570	arg1	data					623:626	interaction data	611:626	interaction data of carbohydrate-binding proteins using carbohydrate microarrays	611:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	3	9	from	oculata	433:439	arg1	polysaccharides					396:410	water-soluble polysaccharides	382:410	water-soluble polysaccharides from Nannochloropsis oculata	382:439	In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity.					
31320077	5	10	theme	sulphated	927:935	arg1	heterorhamnans					937:950	anionic sulphated heterorhamnans	919:950	anionic sulphated heterorhamnans	919:950	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	3	11	theme	water-soluble	382:394	arg1	polysaccharides					396:410	water-soluble polysaccharides	382:410	water-soluble polysaccharides from Nannochloropsis oculata	382:439	In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity.					
31320077	1	12	with	compounds	172:180	arg1	application					198:208	biomedical application	187:208	biomedical application	187:208	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	5	13	theme	anionic	919:925	arg1	heterorhamnans					937:950	anionic sulphated heterorhamnans	919:950	anionic sulphated heterorhamnans	919:950	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	6	14	theme	immunostimulatory	957:973	arg1	assay					975:979	The immunostimulatory assay	953:979	The immunostimulatory assay highlighted that these fractions	953:1012	The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes.					
31320077	2	15	dep	composition	295:305	arg1	the					291:293	the	291:293	the	291:293	Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce.					
31320077	0	16	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	0	17	theme	potential	24:32	arg1	activity					52:59	potential immunostimulatory activity	24:59	potential immunostimulatory activity	24:59	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	1	18	theme	microalgae	122:131	arg1	biotechnology					133:145	microalgae biotechnology	122:145	microalgae biotechnology	122:145	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	5	19	theme	α1→4	900:903	arg1	-mannans					905:912	(α1→4)-mannans	899:912	(α1→4)-mannans	899:912	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	7	20	theme	water-soluble	1078:1090	arg1	polysaccharides					1092:1106	the N. oculata water-soluble polysaccharides	1063:1106	the N. oculata water-soluble polysaccharides	1063:1106	Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.					
31320077	4	21	theme	proteins	652:659	arg1	data					623:626	interaction data	611:626	interaction data of carbohydrate-binding proteins using carbohydrate microarrays	611:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	1	22	theme	biotechnology	133:145	arg1	relevance					109:117	The relevance	105:117	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides,	105:234	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	4	23	theme	carbohydrate-binding	631:650	arg1	proteins					652:659	carbohydrate-binding proteins	631:659	carbohydrate-binding proteins using carbohydrate microarrays	631:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	5	24	theme	N.	844:845	arg1	oculata					847:853	N. oculata	844:853	N. oculata	844:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	4	25	theme	polysaccharides	751:765	arg1	features					725:732	the complex structural features	702:732	the complex structural features of the different polysaccharides	702:765	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	0	26	theme	immunostimulatory	34:50	arg1	activity					52:59	potential immunostimulatory activity	24:59	potential immunostimulatory activity	24:59	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	6	27	theme	murine	1035:1040	arg1	B-lymphocytes					1042:1054	murine B-lymphocytes	1035:1054	murine B-lymphocytes	1035:1054	The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes.					
31320077	7	28	theme	biomedical	1166:1175	arg1	applications					1177:1188	immune-mediated biomedical applications	1150:1188	immune-mediated biomedical applications	1150:1188	Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.					
31320077	5	29	from	-mannans	905:912	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	29	from	-mannans	905:912	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	4	30	theme	different	741:749	arg1	polysaccharides					751:765	the different polysaccharides	737:765	the different polysaccharides	737:765	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	0	31	theme	Nannochloropsis	64:78	arg1	polysaccharides					88:102	Nannochloropsis oculata polysaccharides	64:102	Nannochloropsis oculata polysaccharides	64:102	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	4	32	theme	methylation	585:595	arg1	analysis					597:604	methylation analysis	585:604	methylation analysis	585:604	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	5	33	from	rich	860:863	arg1	-mannans					905:912	(α1→4)-mannans	899:912	(α1→4)-mannans	899:912	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	33	from	rich	860:863	arg1	-glucans					880:887	(β1→3, β1→4)-glucans	868:887	-glucans	880:887	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	33	from	rich	860:863	arg1	α1→3					891:894	α1→3	891:894	α1→3	891:894	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	33	from	rich	860:863	arg1	heterorhamnans					937:950	anionic sulphated heterorhamnans	919:950	anionic sulphated heterorhamnans	919:950	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	4	34	theme	interaction	611:621	arg1	data					623:626	interaction data	611:626	interaction data of carbohydrate-binding proteins using carbohydrate microarrays	611:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	5	35	theme	water-soluble	799:811	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	35	theme	water-soluble	799:811	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	1	36	theme	high-value	161:170	arg1	polysaccharides					219:233	polysaccharides	219:233	polysaccharides	219:233	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	1	36	theme	high-value	161:170	arg1	compounds					172:180	high-value compounds	161:180	high-value compounds	161:180	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	4	37	with	analysis	597:604	arg1	data					623:626	interaction data	611:626	interaction data of carbohydrate-binding proteins using carbohydrate microarrays	611:690	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	4	38	theme	complex	706:712	arg1	features					725:732	the complex structural features	702:732	the complex structural features of the different polysaccharides	702:765	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31320077	5	39	theme	polysaccharides	813:827	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	39	theme	polysaccharides	813:827	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	7	40	theme	oculata	1070:1076	arg1	polysaccharides					1092:1106	the N. oculata water-soluble polysaccharides	1063:1106	the N. oculata water-soluble polysaccharides	1063:1106	Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.					
31320077	3	41	theme	activity	548:555	arg1	terms					521:525	terms	521:525	terms of immunostimulatory activity	521:555	In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity.					
31320077	3	42	theme	immunostimulatory	530:546	arg1	activity					548:555	immunostimulatory activity	530:555	immunostimulatory activity	530:555	In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity.					
31320077	0	43	theme	polysaccharides	88:102	arg1	activity					52:59	potential immunostimulatory activity	24:59	potential immunostimulatory activity	24:59	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	0	43	theme	polysaccharides	88:102	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	5	44	from	heterorhamnans	937:950	arg1	rich					860:863	rich	860:863	rich	860:863	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	5	44	from	heterorhamnans	937:950	arg1	fractions					829:837	the water-soluble polysaccharides fractions	795:837	the water-soluble polysaccharides fractions from N. oculata	795:853	These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans.					
31320077	0	45	theme	oculata	80:86	arg1	polysaccharides					88:102	Nannochloropsis oculata polysaccharides	64:102	Nannochloropsis oculata polysaccharides	64:102	Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.					
31320077	7	46	theme	immune-mediated	1150:1164	arg1	applications					1177:1188	immune-mediated biomedical applications	1150:1188	immune-mediated biomedical applications	1150:1188	Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.					
31320077	1	47	theme	biomedical	187:196	arg1	application					198:208	biomedical application	187:208	biomedical application	187:208	The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing.					
31320077	4	48	theme	structural	714:723	arg1	features					725:732	the complex structural features	702:732	the complex structural features of the different polysaccharides	702:765	A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides.					
31424155	5	0	theme	wall	688:691	arg1	maturation					693:702	conidial cell wall maturation	674:702	conidial cell wall maturation	674:702	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	6	1	theme	cell	925:928	arg1	wall					930:933	the conidial cell wall	912:933	the conidial cell wall mediated by members of the GH55 family	912:972	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	1	2	theme	fumigatus	135:143	arg1	wall					115:118	The cell wall	106:118	The cell wall of Aspergillus fumigatus	106:143	The cell wall of Aspergillus fumigatus is predominantly composed of polysaccharides.					
31424155	6	3	from	conclusion	862:871	arg1	essential					977:985	essential	977:985	essential	977:985	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	5	4	theme	conidia	808:814	arg1	shape					799:803	the shape	795:803	the shape of conidia	795:814	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	0	5	theme	cell	81:84	arg1	morphogenesis					91:103	conidial cell wall morphogenesis	72:103	conidial cell wall morphogenesis	72:103	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	3	6	dep	wherein	417:423	arg1	cleaved					456:462	cleaved	456:462	are cleaved by glycosyl hydrolases	452:485	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	0	7	theme	conidial	72:79	arg1	morphogenesis					91:103	conidial cell wall morphogenesis	72:103	conidial cell wall morphogenesis	72:103	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	6	8	from	essential	977:985	arg1	conclusion					862:871	conclusion	862:871	conclusion	862:871	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	2	9	theme	β	261:261	arg1	glucan					267:272	a branched β(1-3)glucan	250:272	a branched β(1-3)glucan	250:272	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	5	10	theme	conidial	774:781	arg1	separation					783:792	conidial separation	774:792	conidial separation	774:792	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	5	11	theme	family	659:664	arg1	genes					641:645	the six genes	633:645	the six genes of the GH55 family	633:664	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	2	12	theme	branched	252:259	arg1	glucan					267:272	a branched β(1-3)glucan	250:272	a branched β(1-3)glucan	250:272	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	3	13	theme	essential	372:380	arg1	event					382:386	an essential event	369:386	an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases	369:485	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	3	13	theme	essential	372:380	arg1	Softening					339:347	Softening	339:347	Softening of the cell wall	339:364	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	0	14	theme	wall	86:89	arg1	morphogenesis					91:103	conidial cell wall morphogenesis	72:103	conidial cell wall morphogenesis	72:103	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	5	15	theme	development	728:738	arg1	process					740:746	the development process	724:746	the development process	724:746	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	4	16	theme	fungal	584:589	arg1	morphogenesis					591:603	A. fumigatus fungal morphogenesis	571:603	A. fumigatus fungal morphogenesis	571:603	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	6	17	theme	family	967:972	arg1	members					947:953	members	947:953	members of the GH55 family	947:972	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	0	18	theme	Aspergillus	0:10	arg1	glucanases					31:40	Aspergillus fumigatus exoβ(1-3)glucanases	0:40	Aspergillus fumigatus exoβ(1-3)glucanases family GH55	0:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	6	19	theme	GH55	962:965	arg1	family					967:972	the GH55 family	958:972	the GH55 family	958:972	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	5	20	theme	process	740:746	arg1	beginning					711:719	the beginning	707:719	the beginning of the development process	707:746	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	5	21	theme	GH55	654:657	arg1	family					659:664	the GH55 family	650:664	the GH55 family	650:664	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	0	22	theme	exoβ	22:25	arg1	glucanases					31:40	Aspergillus fumigatus exoβ(1-3)glucanases	0:40	Aspergillus fumigatus exoβ(1-3)glucanases family GH55	0:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	3	23	theme	glycosyl	467:474	arg1	hydrolases					476:485	glycosyl hydrolases	467:485	glycosyl hydrolases	467:485	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	6	24	theme	conidial	916:923	arg1	wall					930:933	the conidial cell wall	912:933	the conidial cell wall mediated by members of the GH55 family	912:972	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	4	25	theme	members	560:566	arg1	role					524:527	the role	520:527	the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis	520:603	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	3	26	theme	cell	356:359	arg1	wall					361:364	the cell wall	352:364	the cell wall	352:364	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	5	27	theme	genes	641:645	arg1	deletion					621:628	deletion	621:628	deletion of the six genes of the GH55 family	621:664	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	2	28	theme	cell	225:228	arg1	wall					230:233	the cell wall	221:233	the cell wall	221:233	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	3	29	theme	wall	361:364	arg1	event					382:386	an essential event	369:386	an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases	369:485	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	3	29	theme	wall	361:364	arg1	Softening					339:347	Softening	339:347	Softening of the cell wall	339:364	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	4	30	from	role	524:527	arg1	morphogenesis					591:603	A. fumigatus fungal morphogenesis	571:603	A. fumigatus fungal morphogenesis	571:603	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	0	31	dep	glucanases	31:40	arg1	GH55					49:52	family GH55	42:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55	0:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	3	32	theme	fungal	395:400	arg1	wherein					417:423	wherein	417:423	wherein rigid cell wall structures are cleaved by glycosyl hydrolases	417:485	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	3	32	theme	fungal	395:400	arg1	morphogenesis					402:414	fungal morphogenesis	395:414	fungal morphogenesis	395:414	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	0	33	theme	family	42:47	arg1	GH55					49:52	family GH55	42:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55	0:52	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	3	34	theme	cell	431:434	arg1	structures					441:450	rigid cell wall structures	425:450	rigid cell wall structures	425:450	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	2	35	theme	fibrillar	203:211	arg1	core					213:216	The central fibrillar core	191:216	The central fibrillar core of the cell wall	191:233	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	3	36	theme	wall	436:439	arg1	structures					441:450	rigid cell wall structures	425:450	rigid cell wall structures	425:450	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	0	37	dep	Aspergillus	0:10	arg1	fumigatus					12:20	fumigatus	12:20	fumigatus	12:20	Aspergillus fumigatus exoβ(1-3)glucanases family GH55 are essential for conidial cell wall morphogenesis.					
31424155	2	38	theme	central	195:201	arg1	core					213:216	The central fibrillar core	191:216	The central fibrillar core of the cell wall	191:233	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	4	39	dep	A.	571:572	arg1	fumigatus					574:582	fumigatus	574:582	fumigatus	574:582	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	1	40	theme	cell	110:113	arg1	wall					115:118	The cell wall	106:118	The cell wall of Aspergillus fumigatus	106:143	The cell wall of Aspergillus fumigatus is predominantly composed of polysaccharides.					
31424155	6	41	theme	normal	1009:1014	arg1	dissemination					1016:1028	normal dissemination	1009:1028	normal dissemination	1009:1028	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	4	42	theme	glycosyl	536:543	arg1	members					560:566	the glycosyl hydrolase GH55 members	532:566	the glycosyl hydrolase GH55 members	532:566	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	6	43	dep	reorganisation	878:891	arg1	the					874:876	the	874:876	the	874:876	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	3	44	theme	rigid	425:429	arg1	structures					441:450	rigid cell wall structures	425:450	rigid cell wall structures	425:450	Softening of the cell wall is an essential event during fungal morphogenesis, wherein rigid cell wall structures are cleaved by glycosyl hydrolases.					
31424155	6	45	theme	wall	930:933	arg1	structuring					897:907	structuring	897:907	structuring	897:907	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	6	45	theme	wall	930:933	arg1	reorganisation					878:891	reorganisation	878:891	reorganisation	878:891	In conclusion, the reorganisation and structuring of the conidial cell wall mediated by members of the GH55 family is essential for their maturation, normal dissemination, and germination.					
31424155	5	46	theme	conidial	674:681	arg1	maturation					693:702	conidial cell wall maturation	674:702	conidial cell wall maturation	674:702	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	4	47	theme	A.	571:572	arg1	morphogenesis					591:603	A. fumigatus fungal morphogenesis	571:603	A. fumigatus fungal morphogenesis	571:603	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	5	48	theme	separation	783:792	arg1	abrogation					760:769	abrogation	760:769	abrogation of conidial separation	760:792	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	4	49	theme	GH55	555:558	arg1	members					560:566	the glycosyl hydrolase GH55 members	532:566	the glycosyl hydrolase GH55 members	532:566	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
31424155	5	50	theme	cell	683:686	arg1	maturation					693:702	conidial cell wall maturation	674:702	conidial cell wall maturation	674:702	We showed that deletion of the six genes of the GH55 family stopped conidial cell wall maturation at the beginning of the development process, leading to abrogation of conidial separation: the shape of conidia became ovoid, and germination was delayed.					
31424155	2	51	theme	wall	230:233	arg1	core					213:216	The central fibrillar core	191:216	The central fibrillar core of the cell wall	191:233	The central fibrillar core of the cell wall is composed of a branched β(1-3)glucan, to which the chitin and the galactomannan are covalently bound.					
31424155	1	52	theme	Aspergillus	123:133	arg1	fumigatus					135:143	Aspergillus fumigatus	123:143	Aspergillus fumigatus	123:143	The cell wall of Aspergillus fumigatus is predominantly composed of polysaccharides.					
31424155	4	53	theme	hydrolase	545:553	arg1	members					560:566	the glycosyl hydrolase GH55 members	532:566	the glycosyl hydrolase GH55 members	532:566	In this study, we characterised the role of the glycosyl hydrolase GH55 members in A. fumigatus fungal morphogenesis.					
30986454	3	0	with	interactions	621:632	arg1	polysaccharides					644:658	both polysaccharides	639:658	both polysaccharides	639:658	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	3	1	theme	circular	501:508	arg1	spectra					520:526	Fluorescence, ultraviolet, and circular dichroism spectra	470:526	spectra	520:526	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	2	2	from	water	451:455	arg1	dispersible					436:446	dispersible	436:446	dispersible	436:446	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	3	3	theme	dichroism	510:518	arg1	spectra					520:526	Fluorescence, ultraviolet, and circular dichroism spectra	470:526	spectra	520:526	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	3	4	from	RPs	546:548	arg1	composites					567:576	the resultant composites	553:576	the resultant composites	553:576	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	5	5	theme	potential	997:1005	arg1	applications					1007:1018	their potential applications	991:1018	their potential applications in emulsions or films	991:1040	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	5	6	from	applications	1007:1018	arg1	films					1036:1040	films	1036:1040	films	1036:1040	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	5	6	from	applications	1007:1018	arg1	emulsions					1023:1031	emulsions	1023:1031	emulsions	1023:1031	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	6	7	theme	other	1227:1231	arg1	areas					1233:1237	numerous other areas	1218:1237	numerous other areas	1218:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	5	8	theme	nanoscale	940:948	arg1	discs					950:954	nanoscale discs	940:954	nanoscale discs	940:954	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	3	9	theme	Fluorescence	470:481	arg1	ultraviolet					484:494	Fluorescence, ultraviolet, and circular dichroism spectra	470:526	ultraviolet	484:494	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	6	10	theme	numerous	1218:1225	arg1	areas					1233:1237	numerous other areas	1218:1237	numerous other areas	1218:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	6	11	from	areas	1233:1237	arg1	design					1173:1178	the futuristic design	1158:1178	the futuristic design of functional ingredients in food and numerous other areas	1158:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	1	12	theme	intriguing	215:224	arg1	task					249:252	an intriguing as well as challenging task	212:252	an intriguing as well as challenging task in food industry	212:269	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	1	13	theme	food	123:126	arg1	components					128:137	food components	123:137	food components combining materials properties from both proteins and polysaccharides	123:207	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	6	14	from	ingredients	1194:1204	arg1	food					1209:1212	food	1209:1212	food	1209:1212	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	6	14	from	ingredients	1194:1204	arg1	areas					1233:1237	numerous other areas	1218:1237	numerous other areas	1218:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	5	15	theme	polysaccharide	905:918	arg1	ratios					920:925	the RP:polysaccharide ratios	898:925	tuning the RP:polysaccharide ratios	891:925	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	2	16	theme	dispersible	436:446	arg1	complexes					426:434	trinary complexes	418:434	trinary complexes dispersible in water	418:455	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	6	17	theme	functional	1183:1192	arg1	ingredients					1194:1204	functional ingredients	1183:1204	functional ingredients in food and numerous other areas	1183:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	2	18	theme	rice	319:322	arg1	RPs					334:336	RPs	334:336	RPs	334:336	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	2	18	theme	rice	319:322	arg1	proteins					324:331	rice proteins	319:331	rice proteins (RPs)	319:337	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	2	19	theme	trinary	418:424	arg1	complexes					426:434	trinary complexes	418:434	trinary complexes dispersible in water	418:455	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	0	20	theme	structural	41:50	arg1	folding					52:58	structural folding	41:58	structural folding of rice proteins	41:75	Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays.					
30986454	3	21	theme	resultant	557:565	arg1	composites					567:576	the resultant composites	553:576	the resultant composites	553:576	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	6	22	from	food	1209:1212	arg1	design					1173:1178	the futuristic design	1158:1178	the futuristic design of functional ingredients in food and numerous other areas	1158:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	1	23	theme	challenging	237:247	arg1	task					249:252	an intriguing as well as challenging task	212:252	an intriguing as well as challenging task in food industry	212:269	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	0	24	theme	rice	63:66	arg1	proteins					68:75	rice proteins	63:75	rice proteins	63:75	Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays.					
30986454	4	25	theme	weakened	849:856	arg1	hydrophobicity					858:871	weakened hydrophobicity	849:871	weakened hydrophobicity	849:871	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	4	26	from	polysaccharides	726:740	arg1	moieties					712:719	hydrophilic moieties	700:719	hydrophilic moieties	700:719	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	4	26	from	polysaccharides	726:740	arg1	RPs					692:694	RPs	692:694	RPs	692:694	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	5	27	theme	RP	902:903	arg1	ratios					920:925	the RP:polysaccharide ratios	898:925	tuning the RP:polysaccharide ratios	891:925	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	1	28	from	task	249:252	arg1	industry					262:269	food industry	257:269	food industry	257:269	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	1	29	theme	materials	149:157	arg1	properties					159:168	materials properties	149:168	materials properties	149:168	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	0	30	theme	proteins	68:75	arg1	folding					52:58	structural folding	41:58	structural folding of rice proteins	41:75	Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays.					
30986454	5	31	link	interlinked	959:969	arg1	ribbons					971:977	interlinked ribbons	959:977	interlinked ribbons	959:977	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	4	32	theme	improved	820:827	arg1	charges					837:843	improved surface charges	820:843	improved surface charges	820:843	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	3	33	theme	interactions	621:632	arg1	result					611:616	a result	609:616	a result of interactions with both polysaccharides	609:658	Fluorescence, ultraviolet, and circular dichroism spectra revealed that the RPs in the resultant composites remained partially unfolded as a result of interactions with both polysaccharides.					
30986454	2	34	from	pH 12	387:391	arg1	CMC					368:370	CMC	368:370	CMC	368:370	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	2	34	from	pH 12	387:391	arg1	pectin					377:382	pectin	377:382	pectin at pH 12 prior to neutralization	377:415	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	2	34	from	pH 12	387:391	arg1	carboxymethylcellulose					344:365	carboxymethylcellulose	344:365	carboxymethylcellulose (CMC)	344:371	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	4	35	theme	trinary	747:753	arg1	composites					755:764	the trinary composites	743:764	the trinary composites	743:764	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	4	35	theme	trinary	747:753	arg1	stable					777:782	stable	777:782	stable	777:782	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	6	36	theme	ingredients	1194:1204	arg1	design					1173:1178	the futuristic design	1158:1178	the futuristic design of functional ingredients in food and numerous other areas	1158:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	1	37	theme	food	257:260	arg1	industry					262:269	food industry	257:269	food industry	257:269	Developing food components combining materials properties from both proteins and polysaccharides is an intriguing as well as challenging task in food industry.					
30986454	6	38	from	design	1173:1178	arg1	food					1209:1212	food	1209:1212	food	1209:1212	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	6	38	from	design	1173:1178	arg1	areas					1233:1237	numerous other areas	1218:1237	numerous other areas	1218:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	5	39	dep	tuning	891:896	arg1	ratios					920:925	the RP:polysaccharide ratios	898:925	tuning the RP:polysaccharide ratios	891:925	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	5	40	theme	interlinked	959:969	arg1	ribbons					971:977	interlinked ribbons	959:977	interlinked ribbons	959:977	Additionally, by tuning the RP:polysaccharide ratios, we obtained nanoscale discs or interlinked ribbons, indicating their potential applications in emulsions or films, respectively.					
30986454	6	41	theme	futuristic	1162:1171	arg1	design					1173:1178	the futuristic design	1158:1178	the futuristic design of functional ingredients in food and numerous other areas	1158:1237	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	0	42	theme	structural	89:98	arg1	interplays					100:109	trinary structural interplays	81:109	trinary structural interplays	81:109	Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays.					
30986454	2	43	theme	present	279:285	arg1	study					287:291	the present study	275:291	the present study	275:291	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	6	44	theme	trinary	1084:1090	arg1	complexes					1092:1100	the trinary complexes	1080:1100	the trinary complexes consisting of multi-components	1080:1131	Based on our strategy, the trinary complexes consisting of multi-components can find applications in the futuristic design of functional ingredients in food and numerous other areas.					
30986454	4	45	theme	hydrophilic	700:710	arg1	moieties					712:719	hydrophilic moieties	700:719	hydrophilic moieties	700:719	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	4	46	theme	exposed	669:675	arg1	interiors					677:685	exposed interiors	669:685	exposed interiors	669:685	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30986454	0	47	theme	trinary	81:87	arg1	interplays					100:109	trinary structural interplays	81:109	trinary structural interplays	81:109	Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays.					
30986454	2	48	from	dispersible	436:446	arg1	water					451:455	water	451:455	water	451:455	In the present study, we report that by mixing rice proteins (RPs) with carboxymethylcellulose (CMC) and pectin at pH 12 prior to neutralization, trinary complexes dispersible in water were formed.					
30986454	4	49	theme	surface	829:835	arg1	charges					837:843	improved surface charges	820:843	improved surface charges	820:843	Bearing exposed interiors from RPs and hydrophilic moieties from polysaccharides, the trinary composites were quite stable against aggregation as indicated by improved surface charges and weakened hydrophobicity.					
30584598	0	0	from	mutations	14:22	arg1	SLC35A2					27:33	SLC35A2	27:33	SLC35A2	27:33	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	2	1	from	patients	412:419	arg1	tissues					399:405	matched brain and blood tissues	375:405	matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes	375:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	4	2	theme	study	690:694	arg1	patients					696:703	the 31 (19.3%) study patients	675:703	the 31 (19.3%) study patients	675:703	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	1	3	theme	mild	248:251	arg1	mMCD					291:294	mMCD	291:294	mMCD	291:294	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	3	theme	mild	248:251	arg1	malformation					253:264	mild malformation	248:264	mild malformation of cortical development (mMCD)	248:295	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	5	4	theme	series	916:921	arg1	presence					883:890	the presence	879:890	the presence of an aberrant N-glycan series	879:921	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	2	5	from	patients	447:454	arg1	tissues					399:405	matched brain and blood tissues	375:405	matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes	375:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	1	6	from	structures	167:176	arg1	tissues					193:199	human brain tissues	181:199	human brain tissues	181:199	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	5	7	theme	aberrant	898:905	arg1	series					916:921	an aberrant N-glycan series	895:921	an aberrant N-glycan series	895:921	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	5	8	theme	high	934:937	arg1	degrees					939:945	high degrees	934:945	high degrees of N-acetylglucosamine	934:968	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	5	9	theme	SLC35A2	993:999	arg1	mutations					1001:1009	SLC35A2 mutations	993:1009	SLC35A2 mutations	993:1009	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	3	10	theme	tissue	533:538	arg1	glyco-capture					540:552	tissue glyco-capture	533:552	tissue glyco-capture	533:552	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	5	11	theme	N-acetylglucosamine	950:968	arg1	degrees					939:945	high degrees	934:945	high degrees of N-acetylglucosamine	934:968	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	3	12	theme	spectrometry	570:581	arg1	analysis					583:590	nanoLC/mass spectrometry analysis	558:590	nanoLC/mass spectrometry analysis	558:590	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	6	13	with	epilepsy	1106:1113	arg1	mMCD					1128:1131	mMCD	1128:1131	mMCD	1128:1131	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	6	13	with	epilepsy	1106:1113	arg1	NLFE					1120:1123	NLFE	1120:1123	NLFE	1120:1123	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	3	14	from	N-glycosylation	618:632	arg1	tissue					652:657	affected brain tissue	637:657	affected brain tissue	637:657	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	1	15	theme	cortical	269:276	arg1	development					278:288	cortical development	269:288	cortical development	269:288	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	2	16	theme	pathway	505:511	arg1	genes					513:517	mTOR pathway genes	500:517	mTOR pathway genes	500:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	1	17	theme	somatic	123:129	arg1	mutations					131:139	somatic mutations	123:139	somatic mutations in SLC35A2	123:150	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	4	18	theme	-galactose	829:838	arg1	transporter					840:850	a uridine diphosphate (UDP)-galactose transporter	802:850	a uridine diphosphate (UDP)-galactose transporter	802:850	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	6	19	theme	intractable	1088:1098	arg1	epilepsy					1106:1113	intractable focal epilepsy	1088:1113	intractable focal epilepsy with NLFE or mMCD	1088:1131	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	0	20	gly	N-glycosylation	76:90	arg1	epilepsy					53:60	intractable epilepsy	41:60	intractable epilepsy with aberrant N-glycosylation	41:90	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	1	21	theme	human	181:185	arg1	tissues					193:199	human brain tissues	181:199	human brain tissues	181:199	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	2	22	theme	mTOR	500:503	arg1	genes					513:517	mTOR pathway genes	500:517	mTOR pathway genes	500:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	4	23	theme	splicing	768:775	arg1	mutations					782:790	splicing site mutations	768:790	splicing site mutations	768:790	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	6	24	theme	focal	1100:1104	arg1	epilepsy					1106:1113	intractable focal epilepsy	1088:1113	intractable focal epilepsy with NLFE or mMCD	1088:1131	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	1	25	theme	brain	187:191	arg1	tissues					193:199	human brain tissues	181:199	human brain tissues	181:199	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	0	26	theme	somatic	6:12	arg1	mutations					14:22	Brain somatic mutations	0:22	Brain somatic mutations in SLC35A2	0:33	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	2	27	with	patients	412:419	arg1	mMCD					461:464	mMCD	461:464	mMCD	461:464	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	27	with	patients	412:419	arg1	NLFE					438:441	intractable NLFE	426:441	intractable NLFE	426:441	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	28	theme	whole	311:315	arg1	exome					317:321	Deep whole exome	306:321	Deep whole exome	306:321	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	6	29	dep	CONCLUSION	1012:1021	arg1	suggests					1033:1040	suggests	1033:1040	suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain	1033:1182	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	2	30	theme	brain	383:387	arg1	tissues					399:405	matched brain and blood tissues	375:405	matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes	375:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	0	31	theme	Brain	0:4	arg1	mutations					14:22	Brain somatic mutations	0:22	Brain somatic mutations in SLC35A2	0:33	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	1	32	theme	development	278:288	arg1	mMCD					291:294	mMCD	291:294	mMCD	291:294	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	32	theme	development	278:288	arg1	malformation					253:264	mild malformation	248:264	mild malformation of cortical development (mMCD)	248:295	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	32	theme	development	278:288	arg1	NLFE					239:242	NLFE	239:242	NLFE	239:242	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	32	theme	development	278:288	arg1	epilepsy					229:236	nonlesional focal epilepsy	211:236	nonlesional focal epilepsy (NLFE)	211:243	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	5	33	theme	Glycome	853:859	arg1	analysis					861:868	Glycome analysis	853:868	Glycome analysis	853:868	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	2	34	theme	Deep	306:309	arg1	exome					317:321	Deep whole exome	306:321	Deep whole exome	306:321	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	35	theme	matched	375:381	arg1	tissues					399:405	matched brain and blood tissues	375:405	matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes	375:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	6	36	gly	N-glycosylation	1146:1160	arg1	brain					1178:1182	the affected brain	1165:1182	the affected brain	1165:1182	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	6	37	from	mutations	1061:1069	arg1	SLC35A2					1074:1080	SLC35A2	1074:1080	SLC35A2	1074:1080	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	1	38	from	mutations	131:139	arg1	SLC35A2					144:150	SLC35A2	144:150	SLC35A2	144:150	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	6	39	from	N-glycosylation	1146:1160	arg1	brain					1178:1182	the affected brain	1165:1182	the affected brain	1165:1182	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	2	40	theme	intractable	426:436	arg1	NLFE					438:441	intractable NLFE	426:441	intractable NLFE	426:441	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	4	41	theme	site	777:780	arg1	mutations					782:790	splicing site mutations	768:790	splicing site mutations	768:790	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	3	42	theme	nanoLC/mass	558:568	arg1	analysis					583:590	nanoLC/mass spectrometry analysis	558:590	nanoLC/mass spectrometry analysis	558:590	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	1	43	theme	nonlesional	211:221	arg1	NLFE					239:242	NLFE	239:242	NLFE	239:242	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	43	theme	nonlesional	211:221	arg1	epilepsy					229:236	nonlesional focal epilepsy	211:236	nonlesional focal epilepsy (NLFE)	211:243	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	4	44	theme	patients	696:703	arg1	Six					668:670	Six	668:670	Six	668:670	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	4	44	theme	patients	696:703	arg1	patients					696:703	the 31 (19.3%) study patients	675:703	the 31 (19.3%) study patients	675:703	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	6	45	theme	brain	1047:1051	arg1	mutations					1061:1069	brain somatic mutations	1047:1069	brain somatic mutations in SLC35A2	1047:1080	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	3	46	gly	N-glycosylation	618:632	arg1	tissue					652:657	affected brain tissue	637:657	affected brain tissue	637:657	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	1	47	theme	focal	223:227	arg1	NLFE					239:242	NLFE	239:242	NLFE	239:242	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	1	47	theme	focal	223:227	arg1	epilepsy					229:236	nonlesional focal epilepsy	211:236	nonlesional focal epilepsy (NLFE)	211:243	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30584598	2	48	theme	blood	393:397	arg1	tissues					399:405	matched brain and blood tissues	375:405	matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes	375:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	5	49	with	tissues	980:986	arg1	mutations					1001:1009	SLC35A2 mutations	993:1009	SLC35A2 mutations	993:1009	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	4	50	dep	RESULTS	660:666	arg1	exhibited					705:713	exhibited	705:713	exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter	705:850	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	6	51	theme	somatic	1053:1059	arg1	mutations					1061:1069	brain somatic mutations	1047:1069	brain somatic mutations in SLC35A2	1047:1080	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	2	52	from	mutations	487:495	arg1	genes					513:517	mTOR pathway genes	500:517	mTOR pathway genes	500:517	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	53	theme	sequencing	336:345	arg1	analyses					347:354	targeted sequencing analyses	327:354	targeted sequencing analyses	327:354	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	0	54	theme	intractable	41:51	arg1	epilepsy					53:60	intractable epilepsy	41:60	intractable epilepsy with aberrant N-glycosylation	41:90	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	2	55	with	patients	447:454	arg1	mMCD					461:464	mMCD	461:464	mMCD	461:464	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	55	with	patients	447:454	arg1	NLFE					438:441	intractable NLFE	426:441	intractable NLFE	426:441	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	56	theme	targeted	327:334	arg1	analyses					347:354	targeted sequencing analyses	327:354	targeted sequencing analyses	327:354	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	4	57	from	mutations	726:734	arg1	SLC35A2					739:745	SLC35A2	739:745	SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter	739:850	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	4	58	theme	31	679:680	arg1	patients					696:703	the 31 (19.3%) study patients	675:703	the 31 (19.3%) study patients	675:703	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	3	59	theme	affected	637:644	arg1	tissue					652:657	affected brain tissue	637:657	affected brain tissue	637:657	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	4	60	dep	SLC35A2	739:745	arg1	mutations					782:790	splicing site mutations	768:790	splicing site mutations	768:790	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	4	60	dep	SLC35A2	739:745	arg1	nonsense					755:762	nonsense	755:762	nonsense	755:762	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	5	61	theme	N-glycan	907:914	arg1	series					916:921	an aberrant N-glycan series	895:921	an aberrant N-glycan series	895:921	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	3	62	theme	brain	646:650	arg1	tissue					652:657	affected brain tissue	637:657	affected brain tissue	637:657	Furthermore, tissue glyco-capture and nanoLC/mass spectrometry analysis were performed to examine N-glycosylation in affected brain tissue.					
30584598	0	63	with	epilepsy	53:60	arg1	N-glycosylation					76:90	aberrant N-glycosylation	67:90	aberrant N-glycosylation	67:90	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	4	64	theme	brain-only	715:724	arg1	mutations					726:734	brain-only mutations	715:734	brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter	715:850	RESULTS Six of the 31 (19.3%) study patients exhibited brain-only mutations in SLC35A2 (mostly nonsense and splicing site mutations) encoding a uridine diphosphate (UDP)-galactose transporter.					
30584598	6	65	theme	aberrant	1137:1144	arg1	N-glycosylation					1146:1160	aberrant N-glycosylation	1137:1160	aberrant N-glycosylation in the affected brain	1137:1182	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	0	66	theme	aberrant	67:74	arg1	N-glycosylation					76:90	aberrant N-glycosylation	67:90	aberrant N-glycosylation	67:90	Brain somatic mutations in SLC35A2 cause intractable epilepsy with aberrant N-glycosylation.					
30584598	5	67	theme	brain	974:978	arg1	tissues					980:986	brain tissues	974:986	brain tissues with SLC35A2 mutations	974:1009	Glycome analysis revealed the presence of an aberrant N-glycan series, including high degrees of N-acetylglucosamine, in brain tissues with SLC35A2 mutations.					
30584598	2	68	dep	METHODS	298:304	arg1	exome					317:321	Deep whole exome	306:321	Deep whole exome	306:321	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	2	68	dep	METHODS	298:304	arg1	analyses					347:354	targeted sequencing analyses	327:354	targeted sequencing analyses	327:354	METHODS Deep whole exome and targeted sequencing analyses were conducted for matched brain and blood tissues from patients with intractable NLFE and patients with mMCD who are negative for mutations in mTOR pathway genes.					
30584598	6	69	theme	affected	1169:1176	arg1	brain					1178:1182	the affected brain	1165:1182	the affected brain	1165:1182	CONCLUSION Our study suggests that brain somatic mutations in SLC35A2 cause intractable focal epilepsy with NLFE or mMCD via aberrant N-glycosylation in the affected brain.					
30584598	1	70	theme	N-glycan	158:165	arg1	structures					167:176	N-glycan structures	158:176	N-glycan structures in human brain tissues	158:199	OBJECTIVE To identify whether somatic mutations in SLC35A2 alter N-glycan structures in human brain tissues and cause nonlesional focal epilepsy (NLFE) or mild malformation of cortical development (mMCD).					
30350597	3	0	theme	hydrogels	501:509	arg1	properties					456:465	the mechanical properties	441:465	the mechanical properties	441:465	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	3	0	theme	hydrogels	501:509	arg1	behavior					431:438	drug release behavior	418:438	drug release behavior	418:438	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	3	0	theme	hydrogels	501:509	arg1	stability					484:492	dimensional stability	472:492	dimensional stability	472:492	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	6	1	theme	hydrogel	862:869	arg1	strength					850:857	improved mechanical strength	830:857	improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM	830:923	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	1	2	theme	Injectable	94:103	arg1	hydrogels					105:113	Injectable hydrogels	94:113	Injectable hydrogels	94:113	Injectable hydrogels have shown great potential in bone tissue engineering.					
30350597	4	3	theme	maltodextrin-based	579:596	arg1	system					625:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	Herein, we presented a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system.					
30350597	4	4	theme	injectable	568:577	arg1	system					625:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	Herein, we presented a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system.					
30350597	5	5	theme	Maltodextrin-based	633:650	arg1	micelles					652:659	Maltodextrin-based micelles	633:659	Maltodextrin-based micelles	633:659	Maltodextrin-based micelles were prepared to solubilize and encapsulate SIM.					
30350597	6	6	theme	mechanical	839:848	arg1	strength					850:857	improved mechanical strength	830:857	improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM	830:923	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	6	7	theme	improved	830:837	arg1	strength					850:857	improved mechanical strength	830:857	improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM	830:923	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	0	8	theme	Maltodextrin-Based	11:28	arg1	Composites					47:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites	0:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.	0:92	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.					
30350597	6	9	theme	SIM	921:923	arg1	release					883:889	controlled release	872:889	controlled release	872:889	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	6	9	theme	SIM	921:923	arg1	capability					907:916	osteogenic capability	896:916	osteogenic capability	896:916	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	6	9	theme	SIM	921:923	arg1	hydrogel					862:869	hydrogel	862:869	hydrogel	862:869	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	1	10	theme	great	126:130	arg1	potential					132:140	great potential	126:140	great potential	126:140	Injectable hydrogels have shown great potential in bone tissue engineering.					
30350597	6	11	theme	aldehyde-modified	725:741	arg1	micelles					743:750	The SIM-loaded aldehyde-modified micelles	710:750	The SIM-loaded aldehyde-modified micelles	710:750	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	3	12	theme	release	423:429	arg1	behavior					431:438	drug release behavior	418:438	drug release behavior	418:438	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	0	13	theme	Injectable	0:9	arg1	Composites					47:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites	0:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.	0:92	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.					
30350597	6	14	theme	SIM-loaded	714:723	arg1	micelles					743:750	The SIM-loaded aldehyde-modified micelles	710:750	The SIM-loaded aldehyde-modified micelles	710:750	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	6	15	theme	capability	907:916	arg1	strength					850:857	improved mechanical strength	830:857	improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM	830:923	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	7	16	theme	loaded	989:994	arg1	carriers					996:1003	drug loaded carriers	984:1003	drug loaded carriers	984:1003	In all, this study demonstrated a strategy to incorporate drug loaded carriers into hydrogels for drug delivery and tissue engineering applications.					
30350597	0	17	theme	Micelle/Hydrogel	30:45	arg1	Composites					47:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites	0:56	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.	0:92	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.					
30350597	1	18	theme	bone	145:148	arg1	engineering					157:167	bone tissue engineering	145:167	bone tissue engineering	145:167	Injectable hydrogels have shown great potential in bone tissue engineering.					
30350597	0	19	theme	Simvastatin-Controlled	62:83	arg1	Release					85:91	Simvastatin-Controlled Release	62:91	Simvastatin-Controlled Release	62:91	Injectable Maltodextrin-Based Micelle/Hydrogel Composites for Simvastatin-Controlled Release.					
30350597	2	20	theme	hypolipidemic	198:210	arg1	drug					212:215	a common hypolipidemic drug	189:215	a common hypolipidemic drug	189:215	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	2	20	theme	hypolipidemic	198:210	arg1	Simvastatin					170:180	Simvastatin	170:180	Simvastatin (SIM)	170:186	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	7	21	dep	delivery	1029:1036	arg1	applications					1061:1072	applications	1061:1072	applications	1061:1072	In all, this study demonstrated a strategy to incorporate drug loaded carriers into hydrogels for drug delivery and tissue engineering applications.					
30350597	3	22	theme	mechanical	445:454	arg1	properties					456:465	the mechanical properties	441:465	the mechanical properties	441:465	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	3	23	theme	drug	418:421	arg1	behavior					431:438	drug release behavior	418:438	drug release behavior	418:438	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	2	24	theme	common	191:196	arg1	drug					212:215	a common hypolipidemic drug	189:215	a common hypolipidemic drug	189:215	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	2	24	theme	common	191:196	arg1	Simvastatin					170:180	Simvastatin	170:180	Simvastatin (SIM)	170:186	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	3	25	dep	behavior	431:438	arg1	the					414:416	the	414:416	the	414:416	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	3	26	theme	hydrophobic	308:318	arg1	nature					320:325	its hydrophobic nature	304:325	its hydrophobic nature	304:325	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	1	27	theme	tissue	150:155	arg1	engineering					157:167	bone tissue engineering	145:167	bone tissue engineering	145:167	Injectable hydrogels have shown great potential in bone tissue engineering.					
30350597	4	28	theme	novel	535:539	arg1	design					541:546	a novel design	533:546	a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system	533:630	Herein, we presented a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system.					
30350597	6	29	theme	osteogenic	896:905	arg1	capability					907:916	osteogenic capability	896:916	osteogenic capability	896:916	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	6	30	theme	release	883:889	arg1	strength					850:857	improved mechanical strength	830:857	improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM	830:923	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	2	31	theme	potential	242:250	arg1	agent					252:256	a potential agent	240:256	a potential agent to promote bone regeneration	240:285	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	2	31	theme	potential	242:250	arg1	Simvastatin					170:180	Simvastatin	170:180	Simvastatin (SIM)	170:186	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	3	32	theme	dimensional	472:482	arg1	stability					484:492	dimensional stability	472:492	dimensional stability	472:492	However, due to its hydrophobic nature, the compatibility between SIM and hydrogels is rather poor, thereby greatly affecting the drug release behavior, the mechanical properties, and dimensional stability of the hydrogels.					
30350597	7	33	dep	loaded	989:994	arg1	drug					984:987	drug	984:987	drug	984:987	In all, this study demonstrated a strategy to incorporate drug loaded carriers into hydrogels for drug delivery and tissue engineering applications.					
30350597	4	34	theme	composite	615:623	arg1	system					625:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	Herein, we presented a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system.					
30350597	7	35	theme	tissue	1042:1047	arg1	engineering					1049:1059	tissue engineering	1042:1059	tissue engineering	1042:1059	In all, this study demonstrated a strategy to incorporate drug loaded carriers into hydrogels for drug delivery and tissue engineering applications.					
30350597	6	36	theme	controlled	872:881	arg1	release					883:889	controlled release	872:889	controlled release	872:889	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
30350597	2	37	theme	bone	269:272	arg1	regeneration					274:285	bone regeneration	269:285	bone regeneration	269:285	Simvastatin (SIM), a common hypolipidemic drug, has been suggested as a potential agent to promote bone regeneration.					
30350597	7	38	theme	drug	1024:1027	arg1	delivery					1029:1036	drug delivery	1024:1036	drug delivery	1024:1036	In all, this study demonstrated a strategy to incorporate drug loaded carriers into hydrogels for drug delivery and tissue engineering applications.					
30350597	4	39	theme	micelle/hydrogel	598:613	arg1	system					625:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	an injectable maltodextrin-based micelle/hydrogel composite system	565:630	Herein, we presented a novel design to entrap SIM in an injectable maltodextrin-based micelle/hydrogel composite system.					
30350597	6	40	theme	hydrogel	773:780	arg1	network					782:788	the hydrogel network	769:788	the hydrogel network	769:788	The SIM-loaded aldehyde-modified micelles were anchored to the hydrogel network and served as a cross-linker to realize improved mechanical strength of hydrogel, controlled release, and osteogenic capability of SIM.					
29698512	6	0	theme	bovine	1098:1103	arg1	milk					1105:1108	mature bovine milk	1091:1108	mature bovine milk	1091:1108	Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS.					
29698512	4	1	theme	Glycomics	598:606	arg1	applications					608:619	Glycomics applications	598:619	Glycomics applications	598:619	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	3	2	theme	new	533:535	arg1	technique					537:545	a relatively new technique	520:545	a relatively new technique that has been used mainly in the proteomics field	520:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	3	2	theme	new	533:535	arg1	quantification					444:457	Relative compound quantification	426:457	Relative compound quantification by mass spectral analysis of isobarically labeled samples	426:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	1	3	theme	disease	243:249	arg1	prevention					251:260	disease prevention	243:260	disease prevention	243:260	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	8	4	theme	previous	1616:1623	arg1	study					1625:1629	a previous study	1614:1629	a previous study	1614:1629	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	8	5	from	milk	1573:1576	arg1	abundant					1554:1561	abundant	1554:1561	abundant	1554:1561	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	5	6	theme	isobaric	807:814	arg1	tags					823:826	isobaric glycan tags	807:826	isobaric glycan tags	807:826	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	8	7	gly	fucosylated	1451:1461	arg1	OS					1463:1464	four fucosylated OS	1446:1464	four fucosylated OS	1446:1464	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	5	8	theme	milk	854:857	arg1	OS					859:860	bovine milk OS	847:860	bovine milk OS	847:860	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	9	from	advancement	1664:1674	arg1	ability					1683:1689	our ability to characterize milk OS	1679:1713	our ability to characterize milk OS	1679:1713	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	8	10	with	agreement	1599:1607	arg1	study					1625:1629	a previous study	1614:1629	a previous study	1614:1629	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	4	11	theme	glycans	671:677	arg1	analysis					644:651	analysis	644:651	analysis of protein-linked glycans	644:677	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	5	12	with	column	968:973	arg1	analysis					991:998	subsequent analysis	980:998	subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	980:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	10	13	theme	food	2001:2004	arg1	sources					2006:2012	all food sources	1997:2012	all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large	1997:2135	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	5	14	theme	glycan	816:821	arg1	tags					823:826	isobaric glycan tags	807:826	isobaric glycan tags	807:826	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	8	15	theme	OS	1463:1464	arg1	abundances					1432:1441	the abundances	1428:1441	the abundances of four fucosylated OS	1428:1464	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	7	16	dep	low-abundance	1263:1275	arg1	high-molecular					1278:1291	high-molecular	1278:1291	high-molecular	1278:1291	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	5	17	theme	OS	911:912	arg1	separation					889:898	separation	889:898	separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	889:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	1	18	theme	key	113:115	arg1	factor					117:122	a key factor	111:122	a key factor that influences the infant gut microbial composition	111:175	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	1	18	theme	key	113:115	arg1	oligosaccharides					85:100	Milk oligosaccharides	80:100	Milk oligosaccharides (OS)	80:105	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	6	19	theme	most	1201:1204	arg1	OS					1206:1207	most OS	1201:1207	most OS	1201:1207	Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS.					
29698512	4	20	theme	milk	703:706	arg1	OS					708:709	free milk OS	698:709	free milk OS	698:709	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	7	21	theme	ionization	1237:1246	arg1	efficiency					1248:1257	ionization efficiency	1237:1257	ionization efficiency	1237:1257	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	0	22	theme	milk	19:22	arg1	analysis					40:47	Multiplexed bovine milk oligosaccharide analysis	0:47	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.	0:78	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	1	23	theme	infant	144:149	arg1	composition					165:175	the infant gut microbial composition	140:175	the infant gut microbial composition	140:175	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	8	24	from	Holstein	1479:1486	arg1	milk					1469:1472	milk	1469:1472	milk from Holstein and Jersey cattle	1469:1504	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	5	25	theme	liquid	945:950	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	2	26	theme	complementary	389:401	arg1	techniques					414:423	complementary analytical techniques	389:423	complementary analytical techniques	389:423	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	1	27	theme	Milk	80:83	arg1	factor					117:122	a key factor	111:122	a key factor that influences the infant gut microbial composition	111:175	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	1	27	theme	Milk	80:83	arg1	OS					103:104	OS	103:104	OS	103:104	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	1	27	theme	Milk	80:83	arg1	oligosaccharides					85:100	Milk oligosaccharides	80:100	Milk oligosaccharides (OS)	80:105	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	7	28	theme	bovine	1344:1349	arg1	milk					1351:1354	bovine milk	1344:1354	bovine milk	1344:1354	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	4	29	theme	analytical	749:758	arg1	standards					760:768	analytical standards	749:768	analytical standards	749:768	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	3	30	theme	compound	435:442	arg1	technique					537:545	a relatively new technique	520:545	a relatively new technique that has been used mainly in the proteomics field	520:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	3	30	theme	compound	435:442	arg1	quantification					444:457	Relative compound quantification	426:457	Relative compound quantification by mass spectral analysis of isobarically labeled samples	426:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	0	31	theme	Multiplexed	0:10	arg1	analysis					40:47	Multiplexed bovine milk oligosaccharide analysis	0:47	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.	0:78	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	5	32	from	separation	889:898	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	33	theme	instrumental	1796:1807	arg1	time					1818:1821	reduced instrumental analysis time	1788:1821	reduced instrumental analysis time	1788:1821	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	8	34	theme	compounds	1534:1542	arg1	three					1521:1525	three	1521:1525	three	1521:1525	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	8	34	theme	compounds	1534:1542	arg1	compounds					1534:1542	the compounds	1530:1542	the compounds	1530:1542	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	8	35	from	Jersey	1492:1497	arg1	milk					1469:1472	milk	1469:1472	milk from Holstein and Jersey cattle	1469:1504	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	5	36	theme	graphitized	926:936	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	2	37	theme	techniques	414:423	arg1	host					381:384	a host	379:384	a host of complementary analytical techniques	379:423	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	3	38	theme	spectral	467:474	arg1	analysis					476:483	mass spectral analysis	462:483	mass spectral analysis	462:483	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	5	39	theme	bovine	847:852	arg1	OS					859:860	bovine milk OS	847:860	bovine milk OS	847:860	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	5	40	theme	time-of-flight	1014:1027	arg1	spectrometry					1041:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	41	theme	increased	1827:1835	arg1	efficiency					1856:1865	increased analyte ionization efficiency	1827:1865	increased analyte ionization efficiency	1827:1865	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	0	42	theme	mass	69:72	arg1	tags					74:77	aminoxy tandem mass tags	54:77	aminoxy tandem mass tags	54:77	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	0	43	theme	aminoxy	54:60	arg1	tags					74:77	aminoxy tandem mass tags	54:77	aminoxy tandem mass tags	54:77	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	7	44	gly	fucosylated	1300:1310	arg1	OS					1312:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	5	45	theme	mass	1036:1039	arg1	spectrometry					1041:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	46	theme	ionization	1845:1854	arg1	efficiency					1856:1865	increased analyte ionization efficiency	1827:1865	increased analyte ionization efficiency	1827:1865	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	3	47	theme	labeled	501:507	arg1	samples					509:515	isobarically labeled samples	488:515	isobarically labeled samples	488:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	5	48	theme	subsequent	980:989	arg1	analysis					991:998	subsequent analysis	980:998	subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	980:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	7	49	theme	weight	1293:1298	arg1	OS					1312:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	10	50	theme	improved	1873:1880	arg1	ability					1882:1888	This improved ability	1868:1888	This improved ability to measure differences in bioactive OS abundances in large datasets	1868:1956	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	1	51	theme	microbial	155:163	arg1	composition					165:175	the infant gut microbial composition	140:175	the infant gut microbial composition	140:175	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	1	52	theme	infant	220:225	arg1	development					227:237	healthy infant development	212:237	healthy infant development	212:237	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	10	53	theme	bioactive	1916:1924	arg1	abundances					1929:1938	bioactive OS abundances	1916:1938	bioactive OS abundances	1916:1938	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	8	54	from	abundant	1554:1561	arg1	milk					1573:1576	Jersey milk	1566:1576	Jersey milk	1566:1576	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	0	55	with	analysis	40:47	arg1	tags					74:77	aminoxy tandem mass tags	54:77	aminoxy tandem mass tags	54:77	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	6	56	theme	mature	1091:1096	arg1	milk					1105:1108	mature bovine milk	1091:1108	mature bovine milk	1091:1108	Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS.					
29698512	9	57	theme	novel	1637:1641	arg1	method					1643:1648	This novel method	1632:1648	This novel method	1632:1648	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	10	58	theme	large	1943:1947	arg1	datasets					1949:1956	large datasets	1943:1956	large datasets	1943:1956	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	1	59	theme	gut	151:153	arg1	composition					165:175	the infant gut microbial composition	140:175	the infant gut microbial composition	140:175	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	6	60	theme	variation	1177:1185	arg1	coefficients					1161:1172	coefficients	1161:1172	coefficients of variation	1161:1185	Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS.					
29698512	6	61	dep	injections	1140:1149	arg1	replicate					1130:1138	replicate	1130:1138	replicate	1130:1138	Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS.					
29698512	3	62	theme	proteomics	580:589	arg1	field					591:595	the proteomics field	576:595	the proteomics field	576:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	5	63	theme	OS	859:860	arg1	analysis					835:842	the analysis	831:842	the analysis of bovine milk OS	831:860	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	10	64	from	differences	1901:1911	arg1	abundances					1929:1938	bioactive OS abundances	1916:1938	bioactive OS abundances	1916:1938	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	10	64	from	differences	1901:1911	arg1	datasets					1949:1956	large datasets	1943:1956	large datasets	1943:1956	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	3	65	used	used	561:564	arg2	technique					537:545	a relatively new technique	520:545	a relatively new technique that has been used mainly in the proteomics field	520:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	3	65	used	used	561:564	arg2	quantification					444:457	Relative compound quantification	426:457	Relative compound quantification by mass spectral analysis of isobarically labeled samples	426:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	8	66	theme	fucosylated	1451:1461	arg1	OS					1463:1464	four fucosylated OS	1446:1464	four fucosylated OS	1446:1464	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	4	67	theme	protein-linked	656:669	arg1	glycans					671:677	protein-linked glycans	656:677	protein-linked glycans	656:677	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	5	68	theme	tags	823:826	arg1	use					800:802	the use	796:802	the use of isobaric glycan tags to the analysis of bovine milk OS	796:860	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	69	theme	milk	1707:1710	arg1	OS					1712:1713	milk OS	1707:1713	milk OS	1707:1713	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	10	70	theme	OS	1989:1990	arg1	exploration					1974:1984	exploration	1974:1984	exploration of OS	1974:1990	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	5	71	theme	labeled	903:909	arg1	OS					911:912	labeled OS	903:912	labeled OS	903:912	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	4	72	link	protein-linked	656:669	arg1	glycans					671:677	protein-linked glycans	656:677	protein-linked glycans	656:677	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	2	73	dep	structures	315:324	arg1	the					311:313	the	311:313	the	311:313	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	4	74	theme	free	698:701	arg1	OS					708:709	free milk OS	698:709	free milk OS	698:709	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	7	75	theme	Isobaric	1210:1217	arg1	labeling					1219:1226	Isobaric labeling	1210:1226	Isobaric labeling	1210:1226	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	4	76	theme	OS	708:709	arg1	analysis					686:693	analysis	686:693	analysis of free milk OS	686:709	Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards.					
29698512	10	77	theme	health-guiding	2044:2057	arg1	products					2059:2066	health-guiding products	2044:2066	health-guiding products	2044:2066	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	5	78	theme	carbon	938:943	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	79	theme	isobaric	1759:1766	arg1	labeling					1768:1775	isobaric labeling	1759:1775	isobaric labeling	1759:1775	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	5	80	theme	chromatographic	952:966	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	3	81	theme	mass	462:465	arg1	analysis					476:483	mass spectral analysis	462:483	mass spectral analysis	462:483	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	0	82	theme	bovine	12:17	arg1	analysis					40:47	Multiplexed bovine milk oligosaccharide analysis	0:47	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.	0:78	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	9	83	theme	reduced	1788:1794	arg1	time					1818:1821	reduced instrumental analysis time	1788:1821	reduced instrumental analysis time	1788:1821	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	0	84	theme	oligosaccharide	24:38	arg1	analysis					40:47	Multiplexed bovine milk oligosaccharide analysis	0:47	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.	0:78	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	8	85	dep	Holstein	1479:1486	arg1	cattle					1499:1504	cattle	1499:1504	cattle	1499:1504	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	5	86	theme	porous	919:924	arg1	column					968:973	a porous graphitized carbon liquid chromatographic column	917:973	a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry	917:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	9	87	theme	analysis	1809:1816	arg1	time					1818:1821	reduced instrumental analysis time	1788:1821	reduced instrumental analysis time	1788:1821	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	5	88	theme	quadrupole	1003:1012	arg1	spectrometry					1041:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	3	89	theme	Relative	426:433	arg1	technique					537:545	a relatively new technique	520:545	a relatively new technique that has been used mainly in the proteomics field	520:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	3	89	theme	Relative	426:433	arg1	quantification					444:457	Relative compound quantification	426:457	Relative compound quantification by mass spectral analysis of isobarically labeled samples	426:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	8	90	theme	Jersey	1566:1571	arg1	milk					1573:1576	Jersey milk	1566:1576	Jersey milk	1566:1576	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	2	91	theme	analytical	403:412	arg1	techniques					414:423	complementary analytical techniques	389:423	complementary analytical techniques	389:423	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	0	92	theme	tandem	62:67	arg1	tags					74:77	aminoxy tandem mass tags	54:77	aminoxy tandem mass tags	54:77	Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.					
29698512	5	93	theme	tandem	1029:1034	arg1	spectrometry					1041:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	quadrupole time-of-flight tandem mass spectrometry	1003:1052	In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry.					
29698512	7	94	theme	fucosylated	1300:1310	arg1	OS					1312:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	9	95	theme	analyte	1837:1843	arg1	efficiency					1856:1865	increased analyte ionization efficiency	1827:1865	increased analyte ionization efficiency	1827:1865	This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency.					
29698512	10	96	from	large	2131:2135	arg1	population					2117:2126	the population	2113:2126	the population at large	2113:2135	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	10	96	from	large	2131:2135	arg1	infants					2072:2078	infants	2072:2078	infants	2072:2078	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	10	97	dep	ability	1882:1888	arg1	measure					1893:1899	measure	1893:1899	to measure differences in bioactive OS abundances in large datasets	1890:1956	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	3	98	theme	samples	509:515	arg1	technique					537:545	a relatively new technique	520:545	a relatively new technique that has been used mainly in the proteomics field	520:595	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	3	98	theme	samples	509:515	arg1	quantification					444:457	Relative compound quantification	426:457	Relative compound quantification by mass spectral analysis of isobarically labeled samples	426:515	Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field.					
29698512	1	99	theme	healthy	212:218	arg1	development					227:237	healthy infant development	212:237	healthy infant development	212:237	Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent.					
29698512	7	100	theme	low-abundance	1263:1275	arg1	OS					1312:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	low-abundance, high-molecular weight fucosylated OS	1263:1313	Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature.					
29698512	8	101	theme	general	1591:1597	arg1	agreement					1599:1607	general agreement	1591:1607	general agreement with a previous study	1591:1629	We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study.					
29698512	10	102	theme	OS	1926:1927	arg1	abundances					1929:1938	bioactive OS abundances	1916:1938	bioactive OS abundances	1916:1938	This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.					
29698512	2	103	theme	compounds	360:368	arg1	properties					327:336	properties	327:336	properties	327:336	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	2	103	theme	compounds	360:368	arg1	sources					343:349	sources	343:349	sources	343:349	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
29698512	2	103	theme	compounds	360:368	arg1	structures					315:324	structures	315:324	structures	315:324	Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques.					
31604415	8	0	theme	pathogenic	1166:1175	arg1	strains					1187:1193	three pathogenic bacterial strains	1160:1193	three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia	1160:1258	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	3	1	dep	FTIR	676:679	arg1	analyses					704:711	analyses	704:711	analyses	704:711	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	3	1	dep	FTIR	676:679	arg1	i.e.					671:674	i.e.	671:674	i.e.	671:674	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	8	2	theme	antimicrobial	1127:1139	arg1	activities					1141:1150	antimicrobial activities	1127:1150	antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia	1127:1258	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	1	3	theme	agar	262:265	arg1	matrix					267:272	the agar matrix	258:272	the agar matrix for potential applications in drug delivery and biomedical fields	258:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	0	4	theme	Biomedical	84:93	arg1	Applications					95:106	Potential Biomedical Applications	74:106	Potential Biomedical Applications	74:106	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	6	5	theme	stability	906:914	arg1	range					916:920	the thermal stability range	894:920	the thermal stability range of different composite sheets	894:950	TGA study showed the thermal stability range of different composite sheets.					
31604415	8	6	theme	agar	1086:1089	arg1	composites					1097:1106	The prepared agar based composites	1073:1106	The prepared agar based composites	1073:1106	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	1	7	dep	PURPOSE	109:115	arg1	was					150:152	was	150:152	was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields	150:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	2	8	theme	Agar	353:356	arg1	additives					481:489	additives	481:489	additives in different proportions within the agar matrix	481:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	8	theme	Agar	353:356	arg1	composites					364:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	9	theme	simple	544:549	arg1	method					577:582	a simple thermophysico- mechanical method	542:582	a simple thermophysico- mechanical method	542:582	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	6	10	theme	thermal	898:904	arg1	range					916:920	the thermal stability range	894:920	the thermal stability range of different composite sheets	894:950	TGA study showed the thermal stability range of different composite sheets.					
31604415	7	11	theme	XRD	953:955	arg1	pattern					957:963	XRD pattern	953:963	XRD pattern	953:963	XRD pattern revealed the crystallinity and EDX analysis confirmed the elemental composition of the prepared composites.					
31604415	8	12	theme	prepared	1077:1084	arg1	composites					1097:1106	The prepared agar based composites	1073:1106	The prepared agar based composites	1073:1106	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	2	13	from	additives	481:489	arg1	proportions					504:514	different proportions	494:514	different proportions within the agar matrix	494:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	6	14	theme	composite	935:943	arg1	sheets					945:950	different composite sheets	925:950	different composite sheets	925:950	TGA study showed the thermal stability range of different composite sheets.					
31604415	4	15	theme	component	760:768	arg1	presence					743:750	the presence	739:750	the presence of each component in the agar composite	739:790	The FTIR peaks confirmed the presence of each component in the agar composite.					
31604415	1	16	theme	potential	278:286	arg1	applications					288:299	potential applications	278:299	potential applications in drug delivery and biomedical fields	278:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	9	17	theme	biomedical	1541:1550	arg1	applications					1552:1563	potential biomedical applications	1531:1563	potential biomedical applications	1531:1563	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	5	18	from	distribution	823:834	arg1	composite					866:874	the agar composite	857:874	the agar composite	857:874	SEM images showed the uniform distribution of each component in the agar composite.					
31604415	8	19	dep	strains	1187:1193	arg1	coli					1207:1210	Escherichia coli	1195:1210	Escherichia coli	1195:1210	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	8	19	dep	strains	1187:1193	arg1	aureus					1228:1233	Staphylococcus aureus	1213:1233	Staphylococcus aureus	1213:1233	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	8	19	dep	strains	1187:1193	arg1	pneumonia					1250:1258	Klebsiella pneumonia	1239:1258	Klebsiella pneumonia	1239:1258	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	5	20	theme	agar	861:864	arg1	composite					866:874	the agar composite	857:874	the agar composite	857:874	SEM images showed the uniform distribution of each component in the agar composite.					
31604415	4	21	theme	agar	777:780	arg1	composite					782:790	the agar composite	773:790	the agar composite	773:790	The FTIR peaks confirmed the presence of each component in the agar composite.					
31604415	1	22	theme	biocompatible	164:176	arg1	material					199:206	a biocompatible agar based composite material	162:206	a biocompatible agar based composite material	162:206	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	9	23	theme	prepared	1488:1495	arg1	composites					1508:1517	the prepared agar based composites	1484:1517	the prepared agar based composites	1484:1517	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	2	24	theme	different	494:502	arg1	proportions					504:514	different proportions	494:514	different proportions within the agar matrix	494:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	25	theme	METHODOLOGY	341:351	arg1	additives					481:489	additives	481:489	additives in different proportions within the agar matrix	481:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	25	theme	METHODOLOGY	341:351	arg1	composites					364:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	1	26	theme	agar	178:181	arg1	material					199:206	a biocompatible agar based composite material	162:206	a biocompatible agar based composite material	162:206	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	6	27	theme	TGA	877:879	arg1	study					881:885	TGA study	877:885	TGA study	877:885	TGA study showed the thermal stability range of different composite sheets.					
31604415	1	28	from	applications	288:299	arg1	delivery					309:316	drug delivery	304:316	drug delivery	304:316	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	1	28	from	applications	288:299	arg1	fields					333:338	biomedical fields	322:338	biomedical fields	322:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	2	29	theme	agar	527:530	arg1	matrix					532:537	the agar matrix	523:537	the agar matrix	523:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	3	30	theme	prepared	592:599	arg1	composites					612:621	The as prepared agar based composites	585:621	The as prepared agar based composites	585:621	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	1	31	theme	based	183:187	arg1	material					199:206	a biocompatible agar based composite material	162:206	a biocompatible agar based composite material	162:206	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	2	32	theme	sodium	462:467	arg1	aluminum					469:476	sodium aluminum	462:476	sodium aluminum	462:476	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	8	33	theme	antimicrobial	1295:1307	arg1	activities					1309:1318	efficient antimicrobial activities	1285:1318	efficient antimicrobial activities for all composites	1285:1337	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	4	34	from	presence	743:750	arg1	composite					782:790	the agar composite	773:790	the agar composite	773:790	The FTIR peaks confirmed the presence of each component in the agar composite.					
31604415	3	35	theme	agar	601:604	arg1	composites					612:621	The as prepared agar based composites	585:621	The as prepared agar based composites	585:621	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	1	36	theme	composite	189:197	arg1	material					199:206	a biocompatible agar based composite material	162:206	a biocompatible agar based composite material	162:206	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	5	37	theme	uniform	815:821	arg1	distribution					823:834	the uniform distribution	811:834	the uniform distribution of each component in the agar composite	811:874	SEM images showed the uniform distribution of each component in the agar composite.					
31604415	9	38	theme	agar	1497:1500	arg1	composites					1508:1517	the prepared agar based composites	1484:1517	the prepared agar based composites	1484:1517	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	39	theme	thermal	1426:1432	arg1	nature					1418:1423	the non-toxic nature	1404:1423	the non-toxic nature	1404:1423	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	39	theme	thermal	1426:1432	arg1	stability					1434:1442	thermal stability	1426:1442	thermal stability	1426:1442	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	40	theme	potential	1531:1539	arg1	applications					1552:1563	potential biomedical applications	1531:1563	potential biomedical applications	1531:1563	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	1	41	theme	drug	304:307	arg1	delivery					309:316	drug delivery	304:316	drug delivery	304:316	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	8	42	theme	based	1091:1095	arg1	composites					1097:1106	The prepared agar based composites	1073:1106	The prepared agar based composites	1073:1106	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	2	43	theme	particles	438:446	arg1	incorporation					396:408	the incorporation	392:408	the incorporation of magnetic iron oxide nano particles	392:446	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	43	theme	particles	438:446	arg1	aluminum					469:476	sodium aluminum	462:476	sodium aluminum	462:476	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	43	theme	particles	438:446	arg1	graphite					449:456	graphite	449:456	graphite	449:456	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	0	44	theme	Based	41:45	arg1	Nanocomposite					56:68	Agar Based Magnetic Nanocomposite	36:68	Agar Based Magnetic Nanocomposite	36:68	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	2	45	theme	mechanical	566:575	arg1	method					577:582	a simple thermophysico- mechanical method	542:582	a simple thermophysico- mechanical method	542:582	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	5	46	theme	component	844:852	arg1	distribution					823:834	the uniform distribution	811:834	the uniform distribution of each component in the agar composite	811:874	SEM images showed the uniform distribution of each component in the agar composite.					
31604415	2	47	theme	nano	433:436	arg1	particles					438:446	magnetic iron oxide nano particles	413:446	magnetic iron oxide nano particles	413:446	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	0	48	theme	Agar	36:39	arg1	Nanocomposite					56:68	Agar Based Magnetic Nanocomposite	36:68	Agar Based Magnetic Nanocomposite	36:68	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	9	49	theme	non-toxic	1408:1416	arg1	nature					1418:1423	the non-toxic nature	1404:1423	the non-toxic nature	1404:1423	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	49	theme	non-toxic	1408:1416	arg1	stability					1434:1442	thermal stability	1426:1442	thermal stability	1426:1442	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	49	theme	non-toxic	1408:1416	arg1	properties					1472:1481	excellent antibacterial properties	1448:1481	excellent antibacterial properties	1448:1481	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	2	50	theme	thermophysico-	551:564	arg1	method					577:582	a simple thermophysico- mechanical method	542:582	a simple thermophysico- mechanical method	542:582	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	8	51	theme	bacterial	1177:1185	arg1	strains					1187:1193	three pathogenic bacterial strains	1160:1193	three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia	1160:1258	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	9	52	theme	excellent	1448:1456	arg1	nature					1418:1423	the non-toxic nature	1404:1423	the non-toxic nature	1404:1423	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	52	theme	excellent	1448:1456	arg1	properties					1472:1481	excellent antibacterial properties	1448:1481	excellent antibacterial properties	1448:1481	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	7	53	theme	elemental	1023:1031	arg1	composition					1033:1043	the elemental composition	1019:1043	the elemental composition of the prepared composites	1019:1070	XRD pattern revealed the crystallinity and EDX analysis confirmed the elemental composition of the prepared composites.					
31604415	2	54	theme	oxide	427:431	arg1	nano					433:436	magnetic iron oxide nano	413:436	magnetic iron oxide nano particles	413:446	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	0	55	theme	Nanocomposite	56:68	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	0	55	theme	Nanocomposite	56:68	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	1	56	theme	biomedical	322:331	arg1	fields					333:338	biomedical fields	322:338	biomedical fields	322:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	7	57	theme	prepared	1052:1059	arg1	composites					1061:1070	the prepared composites	1048:1070	the prepared composites	1048:1070	XRD pattern revealed the crystallinity and EDX analysis confirmed the elemental composition of the prepared composites.					
31604415	3	58	theme	based	606:610	arg1	composites					612:621	The as prepared agar based composites	585:621	The as prepared agar based composites	585:621	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	4	59	theme	FTIR	718:721	arg1	peaks					723:727	The FTIR peaks	714:727	The FTIR peaks	714:727	The FTIR peaks confirmed the presence of each component in the agar composite.					
31604415	0	60	theme	Magnetic	47:54	arg1	Nanocomposite					56:68	Agar Based Magnetic Nanocomposite	36:68	Agar Based Magnetic Nanocomposite	36:68	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	2	61	theme	iron	422:425	arg1	nano					433:436	magnetic iron oxide nano	413:436	magnetic iron oxide nano particles	413:446	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	7	62	theme	composites	1061:1070	arg1	composition					1033:1043	the elemental composition	1019:1043	the elemental composition of the prepared composites	1019:1070	XRD pattern revealed the crystallinity and EDX analysis confirmed the elemental composition of the prepared composites.					
31604415	2	63	theme	based	358:362	arg1	additives					481:489	additives	481:489	additives in different proportions within the agar matrix	481:537	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	2	63	theme	based	358:362	arg1	composites					364:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites	341:373	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	9	64	dep	CONCLUSION	1340:1349	arg1	study					1368:1372	the overall study	1356:1372	the overall study	1356:1372	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	6	65	theme	different	925:933	arg1	sheets					945:950	different composite sheets	925:950	different composite sheets	925:950	TGA study showed the thermal stability range of different composite sheets.					
31604415	3	66	theme	different	650:658	arg1	techniques					660:669	different techniques	650:669	different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses	650:711	The as prepared agar based composites were then characterized by different techniques i.e. FTIR, SEM, TGA, XRD and EDX analyses.					
31604415	1	67	theme	present	136:142	arg1	study					144:148	the present study	132:148	the present study	132:148	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	1	68	theme	appropriate	229:239	arg1	additives					241:249	appropriate additives	229:249	appropriate additives	229:249	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	7	69	theme	EDX	996:998	arg1	analysis					1000:1007	EDX analysis	996:1007	EDX analysis	996:1007	XRD pattern revealed the crystallinity and EDX analysis confirmed the elemental composition of the prepared composites.					
31604415	1	70	theme	study	144:148	arg1	purpose					121:127	The purpose	117:127	The purpose of the present study	117:148	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	1	71	theme	additives	241:249	arg1	incorporation					212:224	incorporation	212:224	incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields	212:338	PURPOSE The purpose of the present study was to make a biocompatible agar based composite material via incorporation of appropriate additives within the agar matrix for potential applications in drug delivery and biomedical fields.					
31604415	0	72	theme	Potential	74:82	arg1	Applications					95:106	Potential Biomedical Applications	74:106	Potential Biomedical Applications	74:106	Preparation and Characterization of Agar Based Magnetic Nanocomposite for Potential Biomedical Applications.					
31604415	2	73	theme	magnetic	413:420	arg1	nano					433:436	magnetic iron oxide nano	413:436	magnetic iron oxide nano particles	413:446	METHODOLOGY Agar based composites were prepared by the incorporation of magnetic iron oxide nano particles, graphite and sodium aluminum as additives in different proportions within the agar matrix by a simple thermophysico- mechanical method.					
31604415	9	74	theme	based	1502:1506	arg1	composites					1508:1517	the prepared agar based composites	1484:1517	the prepared agar based composites	1484:1517	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	5	75	theme	SEM	793:795	arg1	images					797:802	SEM images	793:802	SEM images	793:802	SEM images showed the uniform distribution of each component in the agar composite.					
31604415	8	76	theme	efficient	1285:1293	arg1	activities					1309:1318	efficient antimicrobial activities	1285:1318	efficient antimicrobial activities for all composites	1285:1337	The prepared agar based composites were evaluated for antimicrobial activities against three pathogenic bacterial strains Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia and the result indicated efficient antimicrobial activities for all composites.					
31604415	9	77	theme	antibacterial	1458:1470	arg1	nature					1418:1423	the non-toxic nature	1404:1423	the non-toxic nature	1404:1423	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	77	theme	antibacterial	1458:1470	arg1	properties					1472:1481	excellent antibacterial properties	1448:1481	excellent antibacterial properties	1448:1481	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	9	78	theme	overall	1360:1366	arg1	study					1368:1372	the overall study	1356:1372	the overall study	1356:1372	CONCLUSION From the overall study, it was concluded that due to the non-toxic nature, thermal stability and excellent antibacterial properties, the prepared agar based composites can receive potential biomedical applications.					
31604415	6	79	theme	sheets	945:950	arg1	range					916:920	the thermal stability range	894:920	the thermal stability range of different composite sheets	894:950	TGA study showed the thermal stability range of different composite sheets.					
31604415	4	80	attach	presence	743:750	arg1	composite					782:790	the agar composite	773:790	the agar composite	773:790	The FTIR peaks confirmed the presence of each component in the agar composite.					
31604415	4	80	attach	presence	743:750	arg2	component					760:768	each component	755:768	each component	755:768	The FTIR peaks confirmed the presence of each component in the agar composite.					
31145950	5	0	theme	mechanical	788:797	arg1	properties					799:808	mechanical properties	788:808	mechanical properties	788:808	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	4	1	theme	TaC	528:530	arg1	nanoparticles					533:545	tantalum carbide (TaC) nanoparticles	510:545	tantalum carbide (TaC) nanoparticles	510:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	4	2	theme	carbide	519:525	arg1	nanoparticles					533:545	tantalum carbide (TaC) nanoparticles	510:545	tantalum carbide (TaC) nanoparticles	510:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	6	3	theme	storage	917:923	arg1	modulus					925:931	storage modulus	917:931	storage modulus	917:931	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	4	4	theme	tantalum	510:517	arg1	nanoparticles					533:545	tantalum carbide (TaC) nanoparticles	510:545	tantalum carbide (TaC) nanoparticles	510:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	0	5	theme	composite	86:94	arg1	hydrogel					96:103	polysaccharide composite hydrogel	71:103	polysaccharide composite hydrogel	71:103	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	5	6	theme	XRD	633:635	arg1	analysis					645:652	XRD and EDX analysis	633:652	analysis	645:652	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	6	7	theme	bare	823:826	arg1	hydrogel					842:849	bare salecan/PDAAm hydrogel	823:849	bare salecan/PDAAm hydrogel	823:849	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	6	8	theme	7-fold	898:903	arg1	increase					905:912	7-fold increase	898:912	7-fold increase in storage modulus	898:931	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	8	9	theme	salecan/PDAAm/TaC	1194:1210	arg1	hydrogels					1222:1230	salecan/PDAAm/TaC composite hydrogels	1194:1230	salecan/PDAAm/TaC composite hydrogels	1194:1230	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	0	10	from	Study	0:4	arg1	carbide					40:46	tantalum carbide	31:46	tantalum carbide	31:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	5	11	theme	EDX	641:643	arg1	analysis					645:652	XRD and EDX analysis	633:652	analysis	645:652	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	12	theme	TaC	569:571	arg1	nanoparticles					573:585	TaC nanoparticles	569:585	TaC nanoparticles	569:585	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	0	13	from	carbide	40:46	arg1	Study					0:4	Study	0:4	Study of the cell responses in tantalum carbide	0:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	4	14	theme	salecan/poly	455:466	arg1	network					498:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	8	15	theme	Live/Dead	1157:1165	arg1	staining					1167:1174	Live/Dead staining	1157:1174	Live/Dead staining	1157:1174	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	3	16	theme	biomedical	266:275	arg1	applications					277:288	biomedical applications	266:288	biomedical applications	266:288	Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties.					
31145950	6	17	theme	salecan/PDAAm	828:840	arg1	hydrogel					842:849	bare salecan/PDAAm hydrogel	823:849	bare salecan/PDAAm hydrogel	823:849	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	1	18	theme	Agrobacterium	185:197	arg1	sp					199:200	Agrobacterium sp	185:200	Agrobacterium sp	185:200	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	5	19	theme	thermal	766:772	arg1	stability					774:782	thermal stability	766:782	thermal stability	766:782	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	7	20	theme	3T3-L1	1120:1125	arg1	cells					1127:1131	3T3-L1 cells	1120:1131	3T3-L1 cells	1120:1131	More importantly, cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3-L1 cells.					
31145950	4	21	theme	composite	414:422	arg1	hydrogel					424:431	a novel microwave-assisted polymerized composite hydrogel	375:431	a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles	375:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	1	22	theme	sp	199:200	arg1	fermentation					169:180	the fermentation	165:180	the fermentation of Agrobacterium sp	165:200	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	0	23	from	responses	18:26	arg1	carbide					40:46	tantalum carbide	31:46	tantalum carbide	31:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	3	24	theme	hydrogels	252:260	arg1	preparation					237:247	the preparation	233:247	the preparation of hydrogels for biomedical applications	233:288	Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties.					
31145950	4	25	theme	polymerized	402:412	arg1	hydrogel					424:431	a novel microwave-assisted polymerized composite hydrogel	375:431	a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles	375:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	9	26	theme	system	1372:1377	arg1	value					1358:1362	the value	1354:1362	the value of this system for potential application as cell adhesion material	1354:1429	All the results highlight the value of this system for potential application as cell adhesion material.					
31145950	6	27	from	increase	862:869	arg1	modulus					886:892	compressive modulus	874:892	compressive modulus	874:892	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	6	27	from	increase	862:869	arg1	modulus					925:931	storage modulus	917:931	storage modulus	917:931	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	0	28	theme	cell	13:16	arg1	responses					18:26	the cell responses	9:26	the cell responses in tantalum carbide	9:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	8	29	theme	composite	1212:1220	arg1	hydrogels					1222:1230	salecan/PDAAm/TaC composite hydrogels	1194:1230	salecan/PDAAm/TaC composite hydrogels	1194:1230	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	6	30	theme	>9.3-fold	852:860	arg1	increase					862:869	>9.3-fold increase	852:869	>9.3-fold increase in compressive modulus	852:892	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	8	31	theme	normal	1315:1320	arg1	rate					1322:1325	the normal rate	1311:1325	the normal rate	1311:1325	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	6	32	from	increase	905:912	arg1	modulus					886:892	compressive modulus	874:892	compressive modulus	874:892	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	6	32	from	increase	905:912	arg1	modulus					925:931	storage modulus	917:931	storage modulus	917:931	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	9	33	theme	potential	1383:1391	arg1	application					1393:1403	potential application	1383:1403	potential application as cell adhesion material	1383:1429	All the results highlight the value of this system for potential application as cell adhesion material.					
31145950	3	34	theme	prominent	301:309	arg1	properties					342:351	its prominent physicochemical and biological properties	297:351	its prominent physicochemical and biological properties	297:351	Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties.					
31145950	4	35	theme	PDAAm	491:495	arg1	network					498:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	8	36	theme	cell	1267:1270	arg1	adhesion					1272:1279	cell adhesion	1267:1279	cell adhesion	1267:1279	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	7	37	theme	composite	1073:1081	arg1	hydrogels					1083:1091	the composite hydrogels	1069:1091	the composite hydrogels	1069:1091	More importantly, cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3-L1 cells.					
31145950	7	37	theme	composite	1073:1081	arg1	non-toxic					1098:1106	non-toxic	1098:1106	non-toxic	1098:1106	More importantly, cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3-L1 cells.					
31145950	0	38	theme	responses	18:26	arg1	Study					0:4	Study	0:4	Study of the cell responses in tantalum carbide	0:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	6	39	theme	nanoparticles	980:992	arg1	3 mL					968:971	3 mL	968:971	3 mL of TaC nanoparticles dispersion (5%, w/v)	968:1013	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	5	40	theme	remarkable	734:743	arg1	improvement					745:755	a remarkable improvement	732:755	a remarkable improvement in their thermal stability and mechanical properties	732:808	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	41	from	improvement	745:755	arg1	stability					774:782	thermal stability	766:782	thermal stability	766:782	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	41	from	improvement	745:755	arg1	properties					799:808	mechanical properties	788:808	mechanical properties	788:808	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	42	theme	nanoparticles	573:585	arg1	means					680:684	means	680:684	means for structural reinforcement	680:713	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	42	theme	nanoparticles	573:585	arg1	incorporation					552:564	The incorporation	548:564	The incorporation of TaC nanoparticles into the composite hydrogels	548:614	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	5	43	theme	structural	690:699	arg1	reinforcement					701:713	structural reinforcement	690:713	structural reinforcement	690:713	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	6	44	theme	TaC	976:978	arg1	nanoparticles					980:992	TaC nanoparticles dispersion (5%, w/v)	976:1013	TaC nanoparticles dispersion (5%, w/v)	976:1013	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	3	45	theme	biological	331:340	arg1	properties					342:351	its prominent physicochemical and biological properties	297:351	its prominent physicochemical and biological properties	297:351	Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties.					
31145950	7	46	theme	cytotoxicity	1034:1045	arg1	results					1047:1053	cytotoxicity results	1034:1053	cytotoxicity results	1034:1053	More importantly, cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3-L1 cells.					
31145950	1	47	theme	linear	119:124	arg1	polysaccharide					136:149	a linear microbial polysaccharide	117:149	a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp	117:200	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	1	47	theme	linear	119:124	arg1	Salecan					106:112	Salecan	106:112	Salecan	106:112	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	0	48	theme	tantalum	31:38	arg1	carbide					40:46	tantalum carbide	31:46	tantalum carbide	31:46	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	9	49	theme	adhesion	1413:1420	arg1	material					1422:1429	cell adhesion material	1408:1429	cell adhesion material	1408:1429	All the results highlight the value of this system for potential application as cell adhesion material.					
31145950	4	50	theme	diacetone	468:476	arg1	network					498:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	6	51	dep	dispersion	994:1003	arg1	w/v					1010:1012	w/v	1010:1012	w/v	1010:1012	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	6	51	dep	dispersion	994:1003	arg1	%					1007:1007	5%	1006:1007	5%	1006:1007	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	4	52	theme	microwave-assisted	383:400	arg1	hydrogel					424:431	a novel microwave-assisted polymerized composite hydrogel	375:431	a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles	375:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	1	53	theme	microbial	126:134	arg1	polysaccharide					136:149	a linear microbial polysaccharide	117:149	a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp	117:200	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	1	53	theme	microbial	126:134	arg1	Salecan					106:112	Salecan	106:112	Salecan	106:112	Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp.					
31145950	6	54	theme	3 mL	968:971	arg1	addition					956:963	the addition	952:963	the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v)	952:1013	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	3	55	theme	physicochemical	311:325	arg1	properties					342:351	its prominent physicochemical and biological properties	297:351	its prominent physicochemical and biological properties	297:351	Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties.					
31145950	6	56	theme	compressive	874:884	arg1	modulus					886:892	compressive modulus	874:892	compressive modulus	874:892	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	4	57	theme	novel	377:381	arg1	hydrogel					424:431	a novel microwave-assisted polymerized composite hydrogel	375:431	a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles	375:545	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	5	58	theme	composite	596:604	arg1	hydrogels					606:614	the composite hydrogels	592:614	the composite hydrogels	592:614	The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties.					
31145950	6	59	dep	nanoparticles	980:992	arg1	dispersion					994:1003	dispersion	994:1003	TaC nanoparticles dispersion (5%, w/v)	976:1013	Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v).					
31145950	0	60	theme	polysaccharide	71:84	arg1	hydrogel					96:103	polysaccharide composite hydrogel	71:103	polysaccharide composite hydrogel	71:103	Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.					
31145950	4	61	theme	acrylamide	478:487	arg1	network					498:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	salecan/poly(diacetone acrylamide) (PDAAm) network	455:504	Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles.					
31145950	8	62	theme	desired	1243:1249	arg1	environment					1251:1261	a desired environment	1241:1261	a desired environment	1241:1261	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	8	63	theme	Cell	1134:1137	arg1	proliferation					1139:1151	Cell proliferation	1134:1151	Cell proliferation	1134:1151	Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate.					
31145950	9	64	theme	cell	1408:1411	arg1	material					1422:1429	cell adhesion material	1408:1429	cell adhesion material	1408:1429	All the results highlight the value of this system for potential application as cell adhesion material.					
31150225	8	0	theme	traditional	1637:1647	arg1	composites					1663:1672	traditional magnetoactive composites	1637:1672	traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents	1637:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	1	with	viscosity	1465:1473	arg1	inclusion					1484:1492	the inclusion	1480:1492	the inclusion of fillers, particularly suitable for printable materials	1480:1550	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	2	2	theme	film	565:568	arg1	fabrication					570:580	an easy film fabrication	557:580	an easy film fabrication	557:580	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	3	theme	composites	1663:1672	arg1	replacement					1622:1632	the replacement	1618:1632	the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents	1618:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	4	theme	toxic	1734:1738	arg1	solvents					1740:1747	toxic solvents	1734:1747	toxic solvents	1734:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	3	5	theme	mechanical	697:706	arg1	agitation					708:716	mechanical agitation	697:716	mechanical agitation followed by doctor blade casting	697:749	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	1	6	theme	methyl	292:297	arg1	MC					310:311	MC	310:311	MC	310:311	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	6	theme	methyl	292:297	arg1	cellulose					299:307	methyl cellulose	292:307	methyl cellulose (MC)	292:312	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	7	theme	cellulose	199:207	arg1	derivatives					209:219	cellulose derivatives	199:219	cellulose derivatives	199:219	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	3	8	theme	doctor	730:735	arg1	casting					743:749	doctor blade casting	730:749	doctor blade casting	730:749	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	2	9	theme	HPC	532:534	arg1	viscosity					499:507	the viscosity	495:507	the viscosity of water-dissolved MC, HPC, and NaCMC	495:545	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	7	10	from	dependence	1205:1214	arg1	groups					1239:1244	both the functional groups	1219:1244	both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content	1219:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	6	11	contain	containing	1109:1118	arg1	nanocomposites					1094:1107	nanocomposites	1094:1107	nanocomposites containing 50 wt % of nanoparticles	1094:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	6	11	contain	containing	1109:1118	arg2	nanoparticles					1131:1143	nanoparticles	1131:1143	nanoparticles	1131:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	6	11	contain	containing	1109:1118	arg2	%					1126:1126	50 wt %	1120:1126	50 wt % of nanoparticles	1120:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	8	12	theme	viscosity	1465:1473	arg1	decrease					1449:1456	the decrease	1445:1456	the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials	1445:1550	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	3	13	with	films	594:598	arg1	nanoparticles					629:641	homogeneously dispersed nanoparticles	605:641	homogeneously dispersed nanoparticles having concentrations up to 50 wt %	605:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	8	14	dep	composites	1663:1672	arg1	avoiding					1714:1721	avoiding	1714:1721	avoiding the use of toxic solvents	1714:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	14	dep	composites	1663:1672	arg1	based					1674:1678	based	1674:1678	based on petroleum-derived polymers	1674:1708	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	6	15	theme	ferrite	1028:1034	arg1	concentration					1036:1048	cobalt ferrite concentration	1021:1048	cobalt ferrite concentration	1021:1048	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	7	16	theme	functional	1228:1237	arg1	groups					1239:1244	both the functional groups	1219:1244	both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content	1219:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	8	17	link	petroleum-derived	1683:1699	arg1	polymers					1701:1708	petroleum-derived polymers	1683:1708	petroleum-derived polymers	1683:1708	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	1	18	theme	magnetic	175:182	arg1	films					184:188	freestanding magnetic films	162:188	freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	162:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	2	19	theme	MC	528:529	arg1	viscosity					499:507	the viscosity	495:507	the viscosity of water-dissolved MC, HPC, and NaCMC	495:545	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	20	theme	matrices	1360:1367	arg1	character					1335:1343	The renewable character	1321:1343	The renewable character of the hosting matrices	1321:1367	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	1	21	theme	simple	111:116	arg1	route					145:149	a simple and environmentally benign route	109:149	a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	109:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	8	22	theme	suitable	1519:1526	arg1	fillers					1497:1503	fillers	1497:1503	fillers	1497:1503	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	4	23	theme	nanocomposite	763:775	arg1	films					777:781	the nanocomposite films	759:781	the nanocomposite films	759:781	All of the nanocomposite films keep a substantial level of flexibility with elongation at break exceeding 5%.					
31150225	1	24	theme	carboxymethyl	357:369	arg1	NaCMC					382:386	NaCMC	382:386	NaCMC	382:386	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	24	theme	carboxymethyl	357:369	arg1	cellulose					371:379	sodium carboxymethyl cellulose	350:379	sodium carboxymethyl cellulose (NaCMC)	350:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	6	25	theme	g-1	1086:1088	arg1	value					1066:1070	a maximum value	1056:1070	a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles	1056:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	2	26	theme	hydrogen	425:432	arg1	interactions					442:453	" hydrogen bonding interactions	423:453	" hydrogen bonding interactions between polysaccharide chains	423:483	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	27	theme	printable	1532:1540	arg1	materials					1542:1550	printable materials	1532:1550	printable materials	1532:1550	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	0	28	theme	Water-Soluble	0:12	arg1	Derivatives					24:34	Water-Soluble Cellulose Derivatives	0:34	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials	0:85	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials.					
31150225	3	29	dep	%	677:677	arg1	up					665:666	up	665:666	up	665:666	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	7	30	theme	films	1177:1181	arg1	response					1161:1168	The dielectric response	1146:1168	The dielectric response of the films	1146:1181	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	2	31	dep	viscosity	499:507	arg1	lower					489:493	lower	489:493	lower	489:493	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	5	32	theme	interfaces	953:962	arg1	character					925:933	the character	921:933	the character of matrix-CoFe2O4 interfaces	921:962	Halpin-Tsai equations serve to provide further insights on the character of matrix-CoFe2O4 interfaces.					
31150225	2	33	theme	polysaccharide	463:476	arg1	chains					478:483	polysaccharide chains	463:483	polysaccharide chains	463:483	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	6	34	theme	maximum	1058:1064	arg1	value					1066:1070	a maximum value	1056:1070	a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles	1056:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	0	35	theme	Suitable	39:46	arg1	Matrices					48:55	Suitable Matrices	39:55	Suitable Matrices for Multifunctional Materials	39:85	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials.					
31150225	1	36	with	nanoparticles	273:285	arg1	cellulose					329:337	hydroxypropyl cellulose	315:337	hydroxypropyl cellulose (HPC)	315:343	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	36	with	nanoparticles	273:285	arg1	cellulose					299:307	methyl cellulose	292:307	methyl cellulose (MC)	292:312	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	36	with	nanoparticles	273:285	arg1	cellulose					371:379	sodium carboxymethyl cellulose	350:379	sodium carboxymethyl cellulose (NaCMC)	350:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	36	with	nanoparticles	273:285	arg1	MC					310:311	MC	310:311	MC	310:311	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	36	with	nanoparticles	273:285	arg1	HPC					340:342	HPC	340:342	HPC	340:342	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	36	with	nanoparticles	273:285	arg1	NaCMC					382:386	NaCMC	382:386	NaCMC	382:386	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	8	37	theme	magnetic	1571:1578	arg1	performance					1580:1590	the resulting magnetic performance	1557:1590	the resulting magnetic performance	1557:1590	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	7	38	theme	dielectric	1150:1159	arg1	response					1161:1168	The dielectric response	1146:1168	The dielectric response of the films	1146:1181	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	1	39	theme	hydroxypropyl	315:327	arg1	HPC					340:342	HPC	340:342	HPC	340:342	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	39	theme	hydroxypropyl	315:327	arg1	cellulose					329:337	hydroxypropyl cellulose	315:337	hydroxypropyl cellulose (HPC)	315:343	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	6	40	theme	nanoparticles	1131:1143	arg1	nanoparticles					1131:1143	nanoparticles	1131:1143	nanoparticles	1131:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	6	40	theme	nanoparticles	1131:1143	arg1	%					1126:1126	50 wt %	1120:1126	50 wt % of nanoparticles	1120:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	3	41	theme	wt	674:675	arg1	%					677:677	50 wt %	671:677	50 wt %	671:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	4	42	theme	substantial	790:800	arg1	level					802:806	a substantial level	788:806	a substantial level of flexibility with elongation at break exceeding 5%	788:859	All of the nanocomposite films keep a substantial level of flexibility with elongation at break exceeding 5%.					
31150225	8	43	theme	petroleum-derived	1683:1699	arg1	polymers					1701:1708	petroleum-derived polymers	1683:1708	petroleum-derived polymers	1683:1708	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	1	44	theme	ferrite	255:261	arg1	nanoparticles					273:285	cobalt ferrite (CoFe2O4) nanoparticles	248:285	cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	248:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	7	45	theme	ferrite	1291:1297	arg1	content					1312:1318	the ferrite nanoparticle content	1287:1318	the ferrite nanoparticle content	1287:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	7	46	theme	cellulose	1267:1275	arg1	chain					1277:1281	the main cellulose chain	1258:1281	the main cellulose chain	1258:1281	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	1	47	theme	CoFe2O4	264:270	arg1	nanoparticles					273:285	cobalt ferrite (CoFe2O4) nanoparticles	248:285	cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	248:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	48	theme	nanoparticles	273:285	arg1	combination					233:243	the combination	229:243	the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	229:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	2	49	theme	easy	560:563	arg1	fabrication					570:580	an easy film fabrication	557:580	an easy film fabrication	557:580	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	50	theme	magnetoactive	1649:1661	arg1	composites					1663:1672	traditional magnetoactive composites	1637:1672	traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents	1637:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	51	theme	solvents	1740:1747	arg1	use					1727:1729	the use	1723:1729	the use of toxic solvents	1723:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	52	dep	together	1370:1377	arg1	with					1379:1382	with	1379:1382	with	1379:1382	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	6	53	theme	Magnetization	965:977	arg1	saturation					979:988	Magnetization saturation	965:988	Magnetization saturation	965:988	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	3	54	theme	blade	737:741	arg1	casting					743:749	doctor blade casting	730:749	doctor blade casting	730:749	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	5	55	theme	Halpin-Tsai	862:872	arg1	equations					874:882	Halpin-Tsai equations	862:882	Halpin-Tsai equations	862:882	Halpin-Tsai equations serve to provide further insights on the character of matrix-CoFe2O4 interfaces.					
31150225	3	56	contain	having	643:648	arg2	concentrations					650:663	concentrations	650:663	concentrations up to 50 wt %	650:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	3	56	contain	having	643:648	arg1	nanoparticles					629:641	homogeneously dispersed nanoparticles	605:641	homogeneously dispersed nanoparticles having concentrations up to 50 wt %	605:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	5	57	theme	further	901:907	arg1	insights					909:916	further insights	901:916	further insights on the character of matrix-CoFe2O4 interfaces	901:962	Halpin-Tsai equations serve to provide further insights on the character of matrix-CoFe2O4 interfaces.					
31150225	1	58	theme	benign	138:143	arg1	route					145:149	a simple and environmentally benign route	109:149	a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	109:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	2	59	theme	NaCMC	541:545	arg1	viscosity					499:507	the viscosity	495:507	the viscosity of water-dissolved MC, HPC, and NaCMC	495:545	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	7	60	attach	attached	1246:1253	arg2	groups					1239:1244	both the functional groups	1219:1244	both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content	1219:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	7	60	attach	attached	1246:1253	arg1	content					1312:1318	the ferrite nanoparticle content	1287:1318	the ferrite nanoparticle content	1287:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	7	60	attach	attached	1246:1253	arg1	chain					1277:1281	the main cellulose chain	1258:1281	the main cellulose chain	1258:1281	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	3	61	theme	Crack-free	583:592	arg1	films					594:598	Crack-free films	583:598	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt %	583:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	8	62	theme	fillers	1497:1503	arg1	inclusion					1484:1492	the inclusion	1480:1492	the inclusion of fillers, particularly suitable for printable materials	1480:1550	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	63	theme	renewable	1325:1333	arg1	character					1335:1343	The renewable character	1321:1343	The renewable character of the hosting matrices	1321:1367	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	4	64	with	level	802:806	arg1	elongation					828:837	elongation	828:837	elongation at break exceeding 5%	828:859	All of the nanocomposite films keep a substantial level of flexibility with elongation at break exceeding 5%.					
31150225	1	65	theme	freestanding	162:173	arg1	films					184:188	freestanding magnetic films	162:188	freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	162:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	6	66	theme	cobalt	1021:1026	arg1	concentration					1036:1048	cobalt ferrite concentration	1021:1048	cobalt ferrite concentration	1021:1048	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	2	67	theme	water-dissolved	512:526	arg1	MC					528:529	water-dissolved MC	512:529	water-dissolved MC	512:529	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	68	theme	hosting	1352:1358	arg1	matrices					1360:1367	the hosting matrices	1348:1367	the hosting matrices	1348:1367	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	7	69	theme	strong	1198:1203	arg1	dependence					1205:1214	a strong dependence	1196:1214	a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content	1196:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	1	70	theme	sodium	350:355	arg1	NaCMC					382:386	NaCMC	382:386	NaCMC	382:386	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	1	70	theme	sodium	350:355	arg1	cellulose					371:379	sodium carboxymethyl cellulose	350:379	sodium carboxymethyl cellulose (NaCMC)	350:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	6	71	dep	value	1066:1070	arg1	up					1050:1051	up	1050:1051	up	1050:1051	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	0	72	theme	Cellulose	14:22	arg1	Derivatives					24:34	Water-Soluble Cellulose Derivatives	0:34	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials	0:85	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials.					
31150225	4	73	from	break	842:846	arg1	elongation					828:837	elongation	828:837	elongation at break exceeding 5%	828:859	All of the nanocomposite films keep a substantial level of flexibility with elongation at break exceeding 5%.					
31150225	6	74	theme	emu	1082:1084	arg1	g-1					1086:1088	∼24-27 emu g-1	1075:1088	∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles	1075:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	2	75	theme	"	423:423	arg1	interactions					442:453	" hydrogen bonding interactions	423:453	" hydrogen bonding interactions between polysaccharide chains	423:483	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	5	76	from	insights	909:916	arg1	character					925:933	the character	921:933	the character of matrix-CoFe2O4 interfaces	921:962	Halpin-Tsai equations serve to provide further insights on the character of matrix-CoFe2O4 interfaces.					
31150225	5	77	theme	matrix-CoFe2O4	938:951	arg1	interfaces					953:962	matrix-CoFe2O4 interfaces	938:962	matrix-CoFe2O4 interfaces	938:962	Halpin-Tsai equations serve to provide further insights on the character of matrix-CoFe2O4 interfaces.					
31150225	8	78	theme	resulting	1561:1569	arg1	performance					1580:1590	the resulting magnetic performance	1557:1590	the resulting magnetic performance	1557:1590	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	8	79	theme	fabrication	1388:1398	arg1	methods					1400:1406	the fabrication methods	1384:1406	the fabrication methods that solely uses water as a solvent	1384:1442	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	6	80	theme	wt	1123:1124	arg1	nanoparticles					1131:1143	nanoparticles	1131:1143	nanoparticles	1131:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	6	80	theme	wt	1123:1124	arg1	%					1126:1126	50 wt %	1120:1126	50 wt % of nanoparticles	1120:1143	Magnetization saturation increases almost linearly with cobalt ferrite concentration up to a maximum value of ∼24-27 emu g-1 for nanocomposites containing 50 wt % of nanoparticles.					
31150225	7	81	theme	nanoparticle	1299:1310	arg1	content					1312:1318	the ferrite nanoparticle content	1287:1318	the ferrite nanoparticle content	1287:1318	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
31150225	3	82	theme	dispersed	619:627	arg1	nanoparticles					629:641	homogeneously dispersed nanoparticles	605:641	homogeneously dispersed nanoparticles having concentrations up to 50 wt %	605:677	Crack-free films with homogeneously dispersed nanoparticles having concentrations up to 50 wt % are fabricated by mechanical agitation followed by doctor blade casting.					
31150225	0	83	theme	Multifunctional	61:75	arg1	Materials					77:85	Multifunctional Materials	61:85	Multifunctional Materials	61:85	Water-Soluble Cellulose Derivatives as Suitable Matrices for Multifunctional Materials.					
31150225	2	84	theme	bonding	434:440	arg1	interactions					442:453	" hydrogen bonding interactions	423:453	" hydrogen bonding interactions between polysaccharide chains	423:483	Nanoparticles are able to "shield" hydrogen bonding interactions between polysaccharide chains and lower the viscosity of water-dissolved MC, HPC, and NaCMC, allowing an easy film fabrication.					
31150225	8	85	theme	novel	1600:1604	arg1	avenues					1606:1612	novel avenues	1600:1612	novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents	1600:1747	The renewable character of the hosting matrices, together with the fabrication methods that solely uses water as a solvent, the decrease of the viscosity with the inclusion of fillers, particularly suitable for printable materials, and the resulting magnetic performance provide novel avenues for the replacement of traditional magnetoactive composites based on petroleum-derived polymers and avoiding the use of toxic solvents.					
31150225	4	86	theme	flexibility	811:821	arg1	level					802:806	a substantial level	788:806	a substantial level of flexibility with elongation at break exceeding 5%	788:859	All of the nanocomposite films keep a substantial level of flexibility with elongation at break exceeding 5%.					
31150225	1	87	theme	cobalt	248:253	arg1	nanoparticles					273:285	cobalt ferrite (CoFe2O4) nanoparticles	248:285	cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC)	248:387	This work reports on a simple and environmentally benign route to prepare freestanding magnetic films based on cellulose derivatives through the combination of cobalt ferrite (CoFe2O4) nanoparticles with methyl cellulose (MC), hydroxypropyl cellulose (HPC), and sodium carboxymethyl cellulose (NaCMC).					
31150225	7	88	theme	main	1262:1265	arg1	chain					1277:1281	the main cellulose chain	1258:1281	the main cellulose chain	1258:1281	The dielectric response of the films demonstrates a strong dependence on both the functional groups attached to the main cellulose chain and the ferrite nanoparticle content.					
30236667	3	0	theme	significant	641:651	arg1	increase					653:660	a significant increase	639:660	a significant increase in rheological moduli (G' and G″)	639:694	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	4	1	dep	increased	753:761	arg1	whereas					774:780	whereas	774:780	whereas	774:780	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	1	2	theme	unfermented	362:372	arg1	flours					423:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	7	3	theme	UABP	1215:1218	arg1	F1					1226:1227	FABP flour formulation (F1) and UABP flour F1	1183:1227	F1	1226:1227	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	3	4	from	Incorporation	565:577	arg1	dough					624:628	CGF biscuit dough	612:628	CGF biscuit dough	612:628	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	1	5	theme	CGF	287:289	arg1	biscuits					310:317	biscuits	310:317	biscuits	310:317	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	5	theme	CGF	287:289	arg1	dough					300:304	dough	300:304	dough	300:304	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	5	theme	CGF	287:289	arg1	flours					292:297	composite gluten-free (CGF) flours	264:297	composite gluten-free (CGF) flours	264:297	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	2	6	theme	viscosity	545:553	arg1	property					555:562	viscosity property	545:562	viscosity property	545:562	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	1	7	theme	Agaricus	374:381	arg1	polysaccharide					392:405	Agaricus bisporus polysaccharide	374:405	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	6	8	theme	CGF	1115:1117	arg1	formulations					1127:1138	the CGF biscuit formulations	1111:1138	the CGF biscuit formulations	1111:1138	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	9	theme	flour	988:992	arg1	formulation					994:1004	FABP flour formulation	983:1004	FABP flour formulation (F3)	983:1009	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	9	theme	flour	988:992	arg1	F3					1007:1008	F3	1007:1008	F3	1007:1008	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	0	10	theme	fermented	102:110	arg1	flour					161:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	1	11	theme	bisporus	383:390	arg1	polysaccharide					392:405	Agaricus bisporus polysaccharide	374:405	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	6	12	theme	FABP	983:986	arg1	formulation					994:1004	FABP flour formulation	983:1004	FABP flour formulation (F3)	983:1009	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	12	theme	FABP	983:986	arg1	F3					1007:1008	F3	1007:1008	F3	1007:1008	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	4	13	theme	flour	747:751	arg1	Supplementation					723:737	Supplementation	723:737	Supplementation of UABP flour	723:751	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	5	14	theme	CGF	853:855	arg1	formulations					865:876	All CGF biscuit formulations	849:876	All CGF biscuit formulations	849:876	All CGF biscuit formulations exhibited lower fracture strength and hardness compared to the control.					
30236667	0	15	theme	Agaricus	128:135	arg1	flour					161:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	1	16	theme	polysaccharide	392:405	arg1	flours					423:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	7	17	theme	sensory	1145:1151	arg1	result					1164:1169	The sensory evaluation result	1141:1169	The sensory evaluation result	1141:1169	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	6	18	theme	dietary	1064:1070	arg1	fibers					1072:1077	dietary fibers	1064:1077	dietary fibers	1064:1077	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	19	contain	contained	1011:1019	arg1	formulation					994:1004	FABP flour formulation	983:1004	FABP flour formulation (F3)	983:1009	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	19	contain	contained	1011:1019	arg1	flour					973:977	UABP flour	968:977	UABP flour	968:977	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	19	contain	contained	1011:1019	arg1	F3					1007:1008	F3	1007:1008	F3	1007:1008	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	19	contain	contained	1011:1019	arg2	nutrients					1033:1041	the highest nutrients	1021:1041	the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations	1021:1138	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	20	theme	protein	1055:1061	arg1	minerals					1096:1103	minerals	1096:1103	minerals	1096:1103	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	20	theme	protein	1055:1061	arg1	fibers					1072:1077	dietary fibers	1064:1077	dietary fibers	1064:1077	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	20	theme	protein	1055:1061	arg1	terms					1046:1050	terms	1046:1050	terms of protein	1046:1061	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	20	theme	protein	1055:1061	arg1	acids					1086:1090	amino acids	1080:1090	amino acids	1080:1090	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	1	21	theme	functional	183:192	arg1	characteristics					227:241	functional, rheological and physicochemical characteristics	183:241	functional, rheological and physicochemical characteristics	183:241	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	0	22	theme	unfermented	116:126	arg1	flour					161:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	6	23	from	nutrients	1033:1041	arg1	minerals					1096:1103	minerals	1096:1103	minerals	1096:1103	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	23	from	nutrients	1033:1041	arg1	fibers					1072:1077	dietary fibers	1064:1077	dietary fibers	1064:1077	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	23	from	nutrients	1033:1041	arg1	terms					1046:1050	terms	1046:1050	terms of protein	1046:1061	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	23	from	nutrients	1033:1041	arg1	acids					1086:1090	amino acids	1080:1090	amino acids	1080:1090	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	3	24	from	increase	653:660	arg1	δ					719:719	δ	719:719	δ	719:719	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	24	from	increase	653:660	arg1	G					685:685	G'	685:686	G'	685:686	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	24	from	increase	653:660	arg1	G″					692:693	G″	692:693	G″	692:693	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	24	from	increase	653:660	arg1	moduli					677:682	rheological moduli	665:682	rheological moduli (G' and G″)	665:694	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	24	from	increase	653:660	arg1	tan					714:716	tan	714:716	tan (δ)	714:720	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	2	25	theme	flour	529:533	arg1	addition					512:519	addition	512:519	addition of FABP flour	512:533	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	2	26	theme	UABP	463:466	arg1	flour					468:472	UABP flour	463:472	UABP flour	463:472	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	3	27	theme	flours	602:607	arg1	Incorporation					565:577	Incorporation	565:577	Incorporation of both polysaccharide flours in CGF biscuit dough	565:628	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	7	28	theme	evaluation	1153:1162	arg1	result					1164:1169	The sensory evaluation result	1141:1169	The sensory evaluation result	1141:1169	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	1	29	theme	rheological	195:205	arg1	characteristics					227:241	functional, rheological and physicochemical characteristics	183:241	functional, rheological and physicochemical characteristics	183:241	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	0	30	theme	Rheological	0:10	arg1	characteristics					24:38	Rheological and quality characteristics	0:38	characteristics	24:38	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	2	31	theme	flour	453:457	arg1	Addition					431:438	Addition	431:438	Addition of both FABP flour and UABP flour	431:472	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	0	32	theme	polysaccharide	146:159	arg1	flour					161:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	4	33	theme	UABP	742:745	arg1	flour					747:751	UABP flour	742:751	UABP flour	742:751	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	1	34	theme	physicochemical	211:225	arg1	characteristics					227:241	functional, rheological and physicochemical characteristics	183:241	functional, rheological and physicochemical characteristics	183:241	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	0	35	theme	quality	16:22	arg1	characteristics					24:38	Rheological and quality characteristics	0:38	characteristics	24:38	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	6	36	theme	UABP	968:971	arg1	flour					973:977	UABP flour	968:977	UABP flour	968:977	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	0	37	theme	bisporus	137:144	arg1	flour					161:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	fermented and unfermented Agaricus bisporus polysaccharide flour	102:165	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	3	38	theme	polysaccharide	587:600	arg1	flours					602:607	both polysaccharide flours	582:607	both polysaccharide flours	582:607	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	4	39	dep	diameter	822:829	arg1	ratio					842:846	ratio	842:846	ratio	842:846	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	0	40	theme	composite	43:51	arg1	dough					65:69	composite gluten-free dough	43:69	composite gluten-free dough	43:69	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	7	41	theme	flour	1220:1224	arg1	F1					1226:1227	FABP flour formulation (F1) and UABP flour F1	1183:1227	F1	1226:1227	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	2	42	theme	functional	483:492	arg1	properties					494:503	functional properties	483:503	functional properties	483:503	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	3	43	theme	rheological	665:675	arg1	G					685:685	G'	685:686	G'	685:686	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	43	theme	rheological	665:675	arg1	G″					692:693	G″	692:693	G″	692:693	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	43	theme	rheological	665:675	arg1	moduli					677:682	rheological moduli	665:682	rheological moduli (G' and G″)	665:694	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	4	44	theme	flour	806:810	arg1	supplementation					782:796	supplementation	782:796	supplementation of FABP flour	782:810	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	2	45	theme	FABP	524:527	arg1	flour					529:533	FABP flour	524:533	FABP flour	524:533	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	0	46	theme	dough	65:69	arg1	characteristics					24:38	Rheological and quality characteristics	0:38	characteristics	24:38	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	7	47	theme	FABP	1183:1186	arg1	F1					1207:1208	F1	1207:1208	F1	1207:1208	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	7	47	theme	FABP	1183:1186	arg1	formulation					1194:1204	FABP flour formulation	1183:1204	FABP flour formulation	1183:1204	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	3	48	theme	CGF	612:614	arg1	dough					624:628	CGF biscuit dough	612:628	CGF biscuit dough	612:628	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	0	49	theme	gluten-free	53:63	arg1	dough					65:69	composite gluten-free dough	43:69	composite gluten-free dough	43:69	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	2	50	theme	flour	468:472	arg1	Addition					431:438	Addition	431:438	Addition of both FABP flour and UABP flour	431:472	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	3	51	theme	biscuit	616:622	arg1	dough					624:628	CGF biscuit dough	612:628	CGF biscuit dough	612:628	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	7	52	theme	flour	1188:1192	arg1	F1					1207:1208	F1	1207:1208	F1	1207:1208	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	7	52	theme	flour	1188:1192	arg1	formulation					1194:1204	FABP flour formulation	1183:1204	FABP flour formulation	1183:1204	The sensory evaluation result showed that FABP flour formulation (F1) and UABP flour F1 were most acceptable.					
30236667	1	53	dep	polysaccharide	392:405	arg1	UABP					417:420	UABP	417:420	UABP	417:420	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	53	dep	polysaccharide	392:405	arg1	FABP					408:411	FABP	408:411	FABP	408:411	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	6	54	theme	biscuit	1119:1125	arg1	formulations					1127:1138	the CGF biscuit formulations	1111:1138	the CGF biscuit formulations	1111:1138	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	6	55	theme	amino	1080:1084	arg1	acids					1086:1090	amino acids	1080:1090	amino acids	1080:1090	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	3	56	dep	moduli	677:682	arg1	G					685:685	G'	685:686	G'	685:686	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	56	dep	moduli	677:682	arg1	G″					692:693	G″	692:693	G″	692:693	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	56	dep	moduli	677:682	arg1	moduli					677:682	rheological moduli	665:682	rheological moduli (G' and G″)	665:694	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	0	57	theme	biscuits	75:82	arg1	characteristics					24:38	Rheological and quality characteristics	0:38	characteristics	24:38	Rheological and quality characteristics of composite gluten-free dough and biscuits supplemented with fermented and unfermented Agaricus bisporus polysaccharide flour.					
30236667	6	58	theme	highest	1025:1031	arg1	nutrients					1033:1041	the highest nutrients	1021:1041	the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations	1021:1138	Furthermore, both UABP flour and FABP flour formulation (F3) contained the highest nutrients in terms of protein, dietary fibers, amino acids and minerals among the CGF biscuit formulations.					
30236667	4	59	theme	FABP	801:804	arg1	flour					806:810	FABP flour	801:810	FABP flour	801:810	Supplementation of UABP flour increased thickness, whereas supplementation of FABP flour increased diameter and spread ratio.					
30236667	5	60	theme	lower	888:892	arg1	strength					903:910	lower fracture strength	888:910	lower fracture strength	888:910	All CGF biscuit formulations exhibited lower fracture strength and hardness compared to the control.					
30236667	5	61	theme	biscuit	857:863	arg1	formulations					865:876	All CGF biscuit formulations	849:876	All CGF biscuit formulations	849:876	All CGF biscuit formulations exhibited lower fracture strength and hardness compared to the control.					
30236667	1	62	theme	composite	264:272	arg1	biscuits					310:317	biscuits	310:317	biscuits	310:317	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	62	theme	composite	264:272	arg1	dough					300:304	dough	300:304	dough	300:304	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	62	theme	composite	264:272	arg1	flours					292:297	composite gluten-free (CGF) flours	264:297	composite gluten-free (CGF) flours	264:297	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	3	63	from	decrease	702:709	arg1	δ					719:719	δ	719:719	δ	719:719	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	63	from	decrease	702:709	arg1	G					685:685	G'	685:686	G'	685:686	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	63	from	decrease	702:709	arg1	G″					692:693	G″	692:693	G″	692:693	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	63	from	decrease	702:709	arg1	moduli					677:682	rheological moduli	665:682	rheological moduli (G' and G″)	665:694	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	3	63	from	decrease	702:709	arg1	tan					714:716	tan	714:716	tan (δ)	714:720	Incorporation of both polysaccharide flours in CGF biscuit dough revealed a significant increase in rheological moduli (G' and G″) and a decrease in tan (δ).					
30236667	1	64	theme	fermented	348:356	arg1	flours					423:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours	348:428	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	2	65	theme	FABP	448:451	arg1	flour					453:457	FABP flour	448:457	FABP flour	448:457	Addition of both FABP flour and UABP flour improved functional properties, while addition of FABP flour decreased viscosity property.					
30236667	5	66	theme	fracture	894:901	arg1	strength					903:910	lower fracture strength	888:910	lower fracture strength	888:910	All CGF biscuit formulations exhibited lower fracture strength and hardness compared to the control.					
30236667	1	67	theme	gluten-free	274:284	arg1	biscuits					310:317	biscuits	310:317	biscuits	310:317	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	67	theme	gluten-free	274:284	arg1	dough					300:304	dough	300:304	dough	300:304	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30236667	1	67	theme	gluten-free	274:284	arg1	flours					292:297	composite gluten-free (CGF) flours	264:297	composite gluten-free (CGF) flours	264:297	In this study, functional, rheological and physicochemical characteristics were carried out for composite gluten-free (CGF) flours, dough and biscuits, respectively fortified with fermented and unfermented Agaricus bisporus polysaccharide (FABP and UABP) flours.					
30340184	2	0	theme	organic	458:464	arg1	loadings					466:473	The organic loadings	454:473	The organic loadings	454:473	The organic loadings were 0.78 kg COD/(m3·d) (GSBR1), 1.16 kg COD/(m3·d) (GSBR2) and 1.53 kg COD/(m3·d) (GSBR3).					
30340184	2	0	theme	organic	458:464	arg1	m3·d					493:496	m3·d	493:496	m3·d	493:496	The organic loadings were 0.78 kg COD/(m3·d) (GSBR1), 1.16 kg COD/(m3·d) (GSBR2) and 1.53 kg COD/(m3·d) (GSBR3).					
30340184	7	1	from	increase	1001:1008	arg1	end					1028:1030	end	1028:1030	end of the cycle	1028:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	1	from	increase	1001:1008	arg1	EPS					1021:1023	soluble EPS	1013:1023	soluble EPS at end of the cycle	1013:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	2	theme	lowest	1083:1088	arg1	removal					1094:1100	the lowest COD removal	1079:1100	the lowest COD removal	1079:1100	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	3	3	theme	EPS	624:626	arg1	content					628:634	the most stable EPS content	608:634	the most stable EPS content	608:634	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	1	4	theme	sludge	414:419	arg1	GSBR					447:450	GSBR	447:450	GSBR	447:450	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	4	theme	sludge	414:419	arg1	reactor					438:444	granular sludge sequencing batch reactor	405:444	granular sludge sequencing batch reactor (GSBR)	405:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	3	5	contain	had	604:606	arg2	content					628:634	the most stable EPS content	608:634	the most stable EPS content	608:634	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	3	5	contain	had	604:606	arg2	composition					640:650	composition	640:650	composition	640:650	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	3	5	contain	had	604:606	arg1	Granules					567:574	Granules	567:574	Granules with a diameter of 0.5-1 mm	567:602	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	0	6	theme	granule	87:93	arg1	size-fractions					95:108	aerobic granule size-fractions	79:108	aerobic granule size-fractions	79:108	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	7	from	Changes	0:6	arg1	composition					64:74	composition	64:74	composition	64:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	7	from	Changes	0:6	arg1	EPS					47:49	EPS	47:49	EPS	47:49	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	7	from	Changes	0:6	arg1	content					52:58	content	52:58	content	52:58	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	7	from	Changes	0:6	arg1	size-fractions					95:108	aerobic granule size-fractions	79:108	aerobic granule size-fractions	79:108	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	7	from	Changes	0:6	arg1	substances					35:44	extracellular polymeric substances	11:44	extracellular polymeric substances (EPS) content and composition	11:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	6	8	from	EPS	910:912	arg1	period					928:933	the famine period	917:933	the famine period	917:933	The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period.					
30340184	1	9	theme	sequencing	421:430	arg1	GSBR					447:450	GSBR	447:450	GSBR	447:450	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	9	theme	sequencing	421:430	arg1	reactor					438:444	granular sludge sequencing batch reactor	405:444	granular sludge sequencing batch reactor (GSBR)	405:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	5	10	theme	cycle	834:838	arg1	end					823:825	end	823:825	end of the cycle	823:838	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	0	11	from	loads	153:157	arg1	cycles					125:130	reactor cycles	117:130	reactor cycles at different organic loads	117:157	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	12	theme	aerobic	79:85	arg1	size-fractions					95:108	aerobic granule size-fractions	79:108	aerobic granule size-fractions	79:108	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	1	13	theme	various	341:347	arg1	size-fractions					349:362	various size-fractions	341:362	various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	341:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	14	from	substances	309:318	arg1	granules					329:336	granules	329:336	granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	329:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	15	theme	batch	432:436	arg1	GSBR					447:450	GSBR	447:450	GSBR	447:450	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	15	theme	batch	432:436	arg1	reactor					438:444	granular sludge sequencing batch reactor	405:444	granular sludge sequencing batch reactor (GSBR)	405:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	4	16	theme	smallest	657:664	arg1	granules					666:673	The smallest granules	653:673	The smallest granules	653:673	The smallest granules had the largest amount of bound EPS.					
30340184	3	17	with	Granules	567:574	arg1	diameter					583:590	a diameter	581:590	a diameter of 0.5-1 mm	581:602	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	1	18	from	beginning	371:379	arg1	granules					329:336	granules	329:336	granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	329:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	18	from	beginning	371:379	arg1	size-fractions					349:362	various size-fractions	341:362	various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	341:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	19	theme	reactor	438:444	arg1	cycle					396:400	the cycle	392:400	the cycle of granular sludge sequencing batch reactor (GSBR)	392:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	6	20	theme	famine	921:926	arg1	period					928:933	the famine period	917:933	the famine period	917:933	The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period.					
30340184	7	21	theme	cycle	1039:1043	arg1	end					1028:1030	end	1028:1030	end of the cycle	1028:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	22	from	end	1028:1030	arg1	substantial					1054:1064	substantial	1054:1064	substantial	1054:1064	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	22	from	end	1028:1030	arg1	increase					1001:1008	the increase	997:1008	the increase in soluble EPS at end of the cycle	997:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	22	from	end	1028:1030	arg1	EPS					1021:1023	soluble EPS	1013:1023	soluble EPS at end of the cycle	1013:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	7	23	theme	diameter	962:969	arg1	<0.5 mm					971:977	a diameter <0.5 mm	960:977	a diameter <0.5 mm	960:977	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	6	24	theme	soluble	902:908	arg1	EPS					910:912	soluble EPS	902:912	soluble EPS in the famine period	902:933	The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period.					
30340184	5	25	theme	granule	757:763	arg1	diameters					765:773	granule diameters	757:773	granule diameters	757:773	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	3	26	theme	stable	617:622	arg1	content					628:634	the most stable EPS content	608:634	the most stable EPS content	608:634	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	6	27	theme	proteins/polysaccharides	845:868	arg1	ratio					870:874	The proteins/polysaccharides ratio	841:874	The proteins/polysaccharides ratio	841:874	The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period.					
30340184	1	28	from	composition	270:280	arg1	granules					329:336	granules	329:336	granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	329:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	29	from	end	385:387	arg1	granules					329:336	granules	329:336	granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	329:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	29	from	end	385:387	arg1	size-fractions					349:362	various size-fractions	341:362	various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	341:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	0	30	theme	polymeric	25:33	arg1	composition					64:74	composition	64:74	composition	64:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	30	theme	polymeric	25:33	arg1	content					52:58	content	52:58	content	52:58	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	30	theme	polymeric	25:33	arg1	EPS					47:49	EPS	47:49	EPS	47:49	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	30	theme	polymeric	25:33	arg1	substances					35:44	extracellular polymeric substances	11:44	extracellular polymeric substances (EPS) content and composition	11:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	31	theme	extracellular	11:23	arg1	composition					64:74	composition	64:74	composition	64:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	31	theme	extracellular	11:23	arg1	content					52:58	content	52:58	content	52:58	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	31	theme	extracellular	11:23	arg1	EPS					47:49	EPS	47:49	EPS	47:49	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	31	theme	extracellular	11:23	arg1	substances					35:44	extracellular polymeric substances	11:44	extracellular polymeric substances (EPS) content and composition	11:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	32	theme	reactor	117:123	arg1	cycles					125:130	reactor cycles	117:130	reactor cycles at different organic loads	117:157	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	7	33	theme	COD	1090:1092	arg1	removal					1094:1100	the lowest COD removal	1079:1100	the lowest COD removal	1079:1100	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	5	34	theme	loosely-bound	726:738	arg1	EPS					740:742	loosely-bound EPS	726:742	loosely-bound EPS	726:742	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	1	35	from	granules	329:336	arg1	end					385:387	end	385:387	end	385:387	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	35	from	granules	329:336	arg1	size-fractions					349:362	various size-fractions	341:362	various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	341:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	35	from	granules	329:336	arg1	beginning					371:379	the beginning	367:379	the beginning	367:379	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	35	from	granules	329:336	arg1	composition					270:280	the composition	266:280	the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	266:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	36	theme	extracellular	285:297	arg1	substances					309:318	extracellular polymeric substances	285:318	extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	285:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	36	theme	extracellular	285:297	arg1	EPS					321:323	EPS	321:323	EPS	321:323	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	0	37	theme	different	135:143	arg1	loads					153:157	different organic loads	135:157	different organic loads	135:157	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	5	38	theme	EPS	740:742	arg1	EPS					740:742	loosely-bound EPS	726:742	loosely-bound EPS	726:742	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	5	38	theme	EPS	740:742	arg1	amount					716:721	The amount	712:721	The amount of loosely-bound EPS	712:742	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	4	39	contain	had	675:677	arg1	granules					666:673	The smallest granules	653:673	The smallest granules	653:673	The smallest granules had the largest amount of bound EPS.					
30340184	4	39	contain	had	675:677	arg2	EPS					707:709	bound EPS	701:709	bound EPS	701:709	The smallest granules had the largest amount of bound EPS.					
30340184	4	39	contain	had	675:677	arg2	amount					691:696	the largest amount	679:696	the largest amount of bound EPS	679:709	The smallest granules had the largest amount of bound EPS.					
30340184	7	40	theme	soluble	1013:1019	arg1	EPS					1021:1023	soluble EPS	1013:1023	soluble EPS at end of the cycle	1013:1043	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	1	41	theme	polymeric	299:307	arg1	substances					309:318	extracellular polymeric substances	285:318	extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	285:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	41	theme	polymeric	299:307	arg1	EPS					321:323	EPS	321:323	EPS	321:323	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	4	42	theme	EPS	707:709	arg1	amount					691:696	the largest amount	679:696	the largest amount of bound EPS	679:709	The smallest granules had the largest amount of bound EPS.					
30340184	4	42	theme	EPS	707:709	arg1	EPS					707:709	bound EPS	701:709	bound EPS	701:709	The smallest granules had the largest amount of bound EPS.					
30340184	1	43	from	effect	211:216	arg1	composition					270:280	the composition	266:280	the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	266:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	43	from	effect	211:216	arg1	diameters					248:256	granule diameters	240:256	granule diameters	240:256	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	44	theme	substances	309:318	arg1	composition					270:280	the composition	266:280	the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	266:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	7	45	with	granules	946:953	arg1	<0.5 mm					971:977	a diameter <0.5 mm	960:977	a diameter <0.5 mm	960:977	In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.					
30340184	3	46	theme	0.5-1 mm	595:602	arg1	diameter					583:590	a diameter	581:590	a diameter of 0.5-1 mm	581:602	Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition.					
30340184	0	47	theme	organic	145:151	arg1	loads					153:157	different organic loads	135:157	different organic loads	135:157	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	48	dep	substances	35:44	arg1	composition					64:74	composition	64:74	composition	64:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	48	dep	substances	35:44	arg1	content					52:58	content	52:58	content	52:58	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	0	48	dep	substances	35:44	arg1	substances					35:44	extracellular polymeric substances	11:44	extracellular polymeric substances (EPS) content and composition	11:74	Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.					
30340184	1	49	theme	cycle	396:400	arg1	end					385:387	end	385:387	end	385:387	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	49	theme	cycle	396:400	arg1	beginning					371:379	the beginning	367:379	the beginning	367:379	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	5	50	theme	famine	807:812	arg1	phase					814:818	the famine phase	803:818	the famine phase	803:818	The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle.					
30340184	1	51	theme	organic	221:227	arg1	loading					229:235	organic loading	221:235	organic loading	221:235	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	4	52	theme	largest	683:689	arg1	amount					691:696	the largest amount	679:696	the largest amount of bound EPS	679:709	The smallest granules had the largest amount of bound EPS.					
30340184	4	52	theme	largest	683:689	arg1	EPS					707:709	bound EPS	701:709	bound EPS	701:709	The smallest granules had the largest amount of bound EPS.					
30340184	1	53	theme	loading	229:235	arg1	effect					211:216	the effect	207:216	the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR)	207:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	54	theme	granular	405:412	arg1	GSBR					447:450	GSBR	447:450	GSBR	447:450	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	1	54	theme	granular	405:412	arg1	reactor					438:444	granular sludge sequencing batch reactor	405:444	granular sludge sequencing batch reactor (GSBR)	405:451	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30340184	4	55	theme	bound	701:705	arg1	EPS					707:709	bound EPS	701:709	bound EPS	701:709	The smallest granules had the largest amount of bound EPS.					
30340184	1	56	theme	granule	240:246	arg1	diameters					248:256	granule diameters	240:256	granule diameters	240:256	This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR).					
30930270	8	0	theme	cell	1334:1337	arg1	adhesion					1339:1346	P-selectin-mediated cell adhesion	1314:1346	P-selectin-mediated cell adhesion	1314:1346	Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.					
30930270	8	1	theme	better	1282:1287	arg1	ability					1298:1304	better blocking ability	1282:1304	better blocking ability	1282:1304	Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.					
30930270	8	2	theme	P-selectin-mediated	1314:1332	arg1	adhesion					1339:1346	P-selectin-mediated cell adhesion	1314:1346	P-selectin-mediated cell adhesion	1314:1346	Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.					
30930270	0	3	theme	cell	133:136	arg1	adhesion					138:145	P-selectin-mediated cell adhesion	113:145	P-selectin-mediated cell adhesion	113:145	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	7	4	theme	uronic	1138:1143	arg1	acid					1145:1148	uronic acid	1138:1148	uronic acid	1138:1148	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	6	5	theme	flow	1008:1011	arg1	chamber					1013:1019	parallel-plate flow chamber	993:1019	parallel-plate flow chamber	993:1019	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	0	6	theme	P-selectin-mediated	113:131	arg1	adhesion					138:145	P-selectin-mediated cell adhesion	113:145	P-selectin-mediated cell adhesion	113:145	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	5	7	theme	physicochemical	773:787	arg1	characterization					789:804	Their physicochemical characterization	767:804	Their physicochemical characterization	767:804	Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR.					
30930270	7	8	theme	total	1118:1122	arg1	carbohydrate					1124:1135	total carbohydrate	1118:1135	total carbohydrate	1118:1135	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	2	9	theme	algal	324:328	arg1	fucoidans					330:338	brown algal fucoidans	318:338	brown algal fucoidans	318:338	It is reported that brown algal fucoidans can antagonize P-selectin function.					
30930270	4	10	theme	ion-exchange	669:680	arg1	chromatography					682:695	ion-exchange chromatography	669:695	ion-exchange chromatography	669:695	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	3	11	theme	fractionation	389:401	arg1	characterization					423:438	the fractionation and physicochemical characterization	385:438	the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan	385:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	6	12	theme	leukocyte	931:939	arg1	adhesion					941:948	P-selectin-mediated leukocyte adhesion	911:948	P-selectin-mediated leukocyte adhesion	911:948	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	2	13	theme	brown	318:322	arg1	fucoidans					330:338	brown algal fucoidans	318:338	brown algal fucoidans	318:338	It is reported that brown algal fucoidans can antagonize P-selectin function.					
30930270	3	14	theme	fractions	503:511	arg1	screening					481:489	the screening	477:489	the screening of fucoidan fractions with P-selectin antagonistic capability	477:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	3	14	theme	fractions	503:511	arg1	characterization					423:438	the fractionation and physicochemical characterization	385:438	the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan	385:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	6	15	theme	P-selectin-mediated	911:929	arg1	adhesion					941:948	P-selectin-mediated leukocyte adhesion	911:948	P-selectin-mediated leukocyte adhesion	911:948	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	3	16	theme	fucoidan	463:470	arg1	screening					481:489	the screening	477:489	the screening of fucoidan fractions with P-selectin antagonistic capability	477:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	3	16	theme	fucoidan	463:470	arg1	characterization					423:438	the fractionation and physicochemical characterization	385:438	the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan	385:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	3	17	theme	fusiforme	453:461	arg1	fucoidan					463:470	Sargassum fusiforme fucoidan	443:470	Sargassum fusiforme fucoidan	443:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	8	18	theme	fucoidan	1237:1244	arg1	fractions					1246:1254	all the fucoidan fractions	1229:1254	all the fucoidan fractions	1229:1254	Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.					
30930270	0	19	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization of Sargassum fusiforme fucoidan fractions	0:73	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	7	20	contain	possessed	1061:1069	arg1	fractions					1051:1059	fucoidan fractions	1042:1059	fucoidan fractions	1042:1059	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	20	contain	possessed	1061:1069	arg2	weight					1182:1187	molecular weight	1172:1187	molecular weight	1172:1187	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	20	contain	possessed	1061:1069	arg2	properties					1096:1105	distinct physicochemical properties	1071:1105	distinct physicochemical properties	1071:1105	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	20	contain	possessed	1061:1069	arg2	acid					1145:1148	uronic acid	1138:1148	uronic acid	1138:1148	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	20	contain	possessed	1061:1069	arg2	carbohydrate					1124:1135	total carbohydrate	1118:1135	total carbohydrate	1118:1135	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	20	contain	possessed	1061:1069	arg2	compositions					1209:1220	monosaccharide compositions	1194:1220	monosaccharide compositions	1194:1220	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	6	21	theme	fractions	898:906	arg1	capacity					873:880	The inhibitory capacity	858:880	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion	858:948	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	2	22	theme	P-selectin	355:364	arg1	function					366:373	P-selectin function	355:373	P-selectin function	355:373	It is reported that brown algal fucoidans can antagonize P-selectin function.					
30930270	3	23	theme	fucoidan	494:501	arg1	fractions					503:511	fucoidan fractions	494:511	fucoidan fractions with P-selectin antagonistic capability	494:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	0	24	theme	Sargassum	36:44	arg1	fractions					65:73	Sargassum fusiforme fucoidan fractions	36:73	Sargassum fusiforme fucoidan fractions	36:73	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	7	25	theme	physicochemical	1080:1094	arg1	compositions					1209:1220	monosaccharide compositions	1194:1220	monosaccharide compositions	1194:1220	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	25	theme	physicochemical	1080:1094	arg1	carbohydrate					1124:1135	total carbohydrate	1118:1135	total carbohydrate	1118:1135	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	25	theme	physicochemical	1080:1094	arg1	acid					1145:1148	uronic acid	1138:1148	uronic acid	1138:1148	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	25	theme	physicochemical	1080:1094	arg1	weight					1182:1187	molecular weight	1172:1187	molecular weight	1172:1187	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	25	theme	physicochemical	1080:1094	arg1	properties					1096:1105	distinct physicochemical properties	1071:1105	distinct physicochemical properties	1071:1105	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	5	26	theme	chemical	824:831	arg1	methods					833:839	chemical methods	824:839	chemical methods	824:839	Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR.					
30930270	5	26	theme	chemical	824:831	arg1	FT-IR					851:855	FT-IR	851:855	FT-IR	851:855	Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR.					
30930270	5	26	theme	chemical	824:831	arg1	HPLC					842:845	HPLC	842:845	HPLC	842:845	Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR.					
30930270	6	27	theme	fucoidan	889:896	arg1	fractions					898:906	the fucoidan fractions	885:906	the fucoidan fractions	885:906	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	3	28	theme	Sargassum	443:451	arg1	fucoidan					463:470	Sargassum fusiforme fucoidan	443:470	Sargassum fusiforme fucoidan	443:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	4	29	theme	fusiforme	647:655	arg1	fucoidan					657:664	the S. fusiforme fucoidan	640:664	the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions	640:764	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	0	30	theme	fucoidan	56:63	arg1	fractions					65:73	Sargassum fusiforme fucoidan fractions	36:73	Sargassum fusiforme fucoidan fractions	36:73	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	4	31	theme	S.	644:645	arg1	fucoidan					657:664	the S. fusiforme fucoidan	640:664	the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions	640:764	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	4	32	theme	fucoidan	747:754	arg1	fractions					756:764	eight fucoidan fractions	741:764	eight fucoidan fractions	741:764	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	1	33	theme	promising	220:228	arg1	P-selectin					148:157	P-selectin	148:157	P-selectin	148:157	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	1	33	theme	promising	220:228	arg1	target					230:235	a promising target	218:235	a promising target for the therapeutics of acute inflammatory-related diseases	218:295	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	3	34	theme	P-selectin	518:527	arg1	capability					542:551	P-selectin antagonistic capability	518:551	P-selectin antagonistic capability	518:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	0	35	theme	fusiforme	46:54	arg1	fractions					65:73	Sargassum fusiforme fucoidan fractions	36:73	Sargassum fusiforme fucoidan fractions	36:73	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	6	36	theme	parallel-plate	993:1006	arg1	chamber					1013:1019	parallel-plate flow chamber	993:1019	parallel-plate flow chamber	993:1019	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	3	37	theme	antagonistic	529:540	arg1	capability					542:551	P-selectin antagonistic capability	518:551	P-selectin antagonistic capability	518:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	3	38	theme	physicochemical	407:421	arg1	characterization					423:438	the fractionation and physicochemical characterization	385:438	the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan	385:470	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	8	39	theme	blocking	1289:1296	arg1	ability					1298:1304	better blocking ability	1282:1304	better blocking ability	1282:1304	Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.					
30930270	6	40	from	capacity	873:880	arg1	adhesion					941:948	P-selectin-mediated leukocyte adhesion	911:948	P-selectin-mediated leukocyte adhesion	911:948	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	0	41	theme	fractions	65:73	arg1	effect					98:103	their antagonistic effect	79:103	their antagonistic effect against P-selectin-mediated cell adhesion	79:145	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	0	41	theme	fractions	65:73	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization of Sargassum fusiforme fucoidan fractions	0:73	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	3	42	with	fractions	503:511	arg1	capability					542:551	P-selectin antagonistic capability	518:551	P-selectin antagonistic capability	518:551	However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated.					
30930270	6	43	theme	adhesion	974:981	arg1	assay					983:987	static adhesion assay	967:987	static adhesion assay	967:987	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	6	44	theme	inhibitory	862:871	arg1	capacity					873:880	The inhibitory capacity	858:880	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion	858:948	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	1	45	theme	mediated	160:167	arg1	P-selectin					148:157	P-selectin	148:157	P-selectin	148:157	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	1	45	theme	mediated	160:167	arg1	adhesion					169:176	mediated adhesion	160:176	mediated adhesion between endothelium and neutrophils	160:212	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	7	46	theme	distinct	1071:1078	arg1	compositions					1209:1220	monosaccharide compositions	1194:1220	monosaccharide compositions	1194:1220	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	46	theme	distinct	1071:1078	arg1	carbohydrate					1124:1135	total carbohydrate	1118:1135	total carbohydrate	1118:1135	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	46	theme	distinct	1071:1078	arg1	acid					1145:1148	uronic acid	1138:1148	uronic acid	1138:1148	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	46	theme	distinct	1071:1078	arg1	weight					1182:1187	molecular weight	1172:1187	molecular weight	1172:1187	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	46	theme	distinct	1071:1078	arg1	properties					1096:1105	distinct physicochemical properties	1071:1105	distinct physicochemical properties	1071:1105	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	4	47	theme	size	701:704	arg1	chromatography					716:729	size exclusion chromatography	701:729	size exclusion chromatography	701:729	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	7	48	theme	monosaccharide	1194:1207	arg1	compositions					1209:1220	monosaccharide compositions	1194:1220	monosaccharide compositions	1194:1220	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	6	49	theme	static	967:972	arg1	assay					983:987	static adhesion assay	967:987	static adhesion assay	967:987	The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber.					
30930270	7	50	theme	sulfate	1154:1160	arg1	contents					1162:1169	sulfate contents	1154:1169	sulfate contents	1154:1169	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	7	51	theme	fucoidan	1042:1049	arg1	fractions					1051:1059	fucoidan fractions	1042:1059	fucoidan fractions	1042:1059	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	1	52	theme	acute	261:265	arg1	diseases					288:295	acute inflammatory-related diseases	261:295	acute inflammatory-related diseases	261:295	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	0	53	theme	antagonistic	85:96	arg1	effect					98:103	their antagonistic effect	79:103	their antagonistic effect against P-selectin-mediated cell adhesion	79:145	Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion.					
30930270	7	54	theme	molecular	1172:1180	arg1	weight					1182:1187	molecular weight	1172:1187	molecular weight	1172:1187	Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions.					
30930270	1	55	theme	inflammatory-related	267:286	arg1	diseases					288:295	acute inflammatory-related diseases	261:295	acute inflammatory-related diseases	261:295	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30930270	4	56	theme	exclusion	706:714	arg1	chromatography					716:729	size exclusion chromatography	701:729	size exclusion chromatography	701:729	In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions.					
30930270	1	57	theme	diseases	288:295	arg1	therapeutics					245:256	the therapeutics	241:256	the therapeutics of acute inflammatory-related diseases	241:295	P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases.					
30827593	5	0	theme	unsaturated	694:704	arg1	acid					719:722	unsaturated galacturonic acid	694:722	unsaturated galacturonic acid	694:722	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	4	1	dep	rhamnogalacturonan	589:606	arg1	1,5-					614:617	1,5-	614:617	1,5-	614:617	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	4	1	dep	rhamnogalacturonan	589:606	arg1	I					608:608	I	608:608	I	608:608	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	0	2	from	characterisation	11:26	arg1	skins					69:73	roasted hazelnut skins	52:73	roasted hazelnut skins	52:73	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	5	3	theme	galacturonic	706:717	arg1	acid					719:722	unsaturated galacturonic acid	694:722	unsaturated galacturonic acid	694:722	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	5	4	theme	acid	719:722	arg1	presence					682:689	The presence	678:689	The presence	678:689	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	3	5	dep	1,5-	493:496	arg1	polysaccharides					527:541	polysaccharides	527:541	polysaccharides	527:541	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	0	6	from	skins	69:73	arg1	polysaccharides					31:45	polysaccharides	31:45	polysaccharides from roasted hazelnut skins	31:73	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	0	6	from	skins	69:73	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation of polysaccharides from roasted hazelnut skins.	0:74	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	3	7	theme	fraction	348:355	arg1	1W					357:358	The fraction 1W	344:358	The fraction 1W	344:358	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	1	8	theme	polysaccharide	80:93	arg1	fractions					95:103	Two polysaccharide fractions	76:103	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A,	76:155	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	3	9	theme	arabinose	461:469	arg1	chains					476:481	arabinose side chains	461:481	arabinose side chains	461:481	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	0	10	theme	hazelnut	60:67	arg1	skins					69:73	roasted hazelnut skins	52:73	roasted hazelnut skins	52:73	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	4	11	theme	1,3,5-arabinan	620:633	arg1	1A					557:558	The fraction 1A	544:558	The fraction 1A	544:558	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	4	11	theme	1,3,5-arabinan	620:633	arg1	mixture					565:571	a mixture	563:571	a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides	563:675	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	1	12	theme	hazelnut	180:187	arg1	skins					189:193	the hazelnut skins	176:193	the hazelnut skins	176:193	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	1	13	attach	isolated	162:169	arg1	skins					189:193	the hazelnut skins	176:193	the hazelnut skins	176:193	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	1	13	attach	isolated	162:169	arg2	fractions					95:103	Two polysaccharide fractions	76:103	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A,	76:155	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	0	14	theme	Structural	0:9	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation of polysaccharides from roasted hazelnut skins.	0:74	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	0	15	theme	polysaccharides	31:45	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation of polysaccharides from roasted hazelnut skins.	0:74	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	5	16	theme	molecular	753:761	arg1	weights					763:769	the molecular weights	749:769	the molecular weights	749:769	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	3	17	with	polysaccharide	397:410	arg1	blocks					438:443	rhamnogalacturonan I blocks	417:443	rhamnogalacturonan I blocks	417:443	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	3	18	theme	pectic	390:395	arg1	polysaccharide					397:410	methyl-esterified pectic polysaccharide	372:410	methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks	372:443	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	4	19	theme	rhamnogalacturonan	589:606	arg1	1A					557:558	The fraction 1A	544:558	The fraction 1A	544:558	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	4	19	theme	rhamnogalacturonan	589:606	arg1	mixture					565:571	a mixture	563:571	a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides	563:675	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	5	20	theme	weights	763:769	arg1	acid					719:722	unsaturated galacturonic acid	694:722	unsaturated galacturonic acid	694:722	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	5	20	theme	weights	763:769	arg1	heterogeneity					732:744	the heterogeneity	728:744	the heterogeneity of the molecular weights	728:769	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	1	21	theme	water	133:137	arg1	1W					139:140	water 1W	133:140	water 1W	133:140	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	0	22	theme	roasted	52:58	arg1	skins					69:73	roasted hazelnut skins	52:73	roasted hazelnut skins	52:73	Structural characterisation of polysaccharides from roasted hazelnut skins.					
30827593	4	23	theme	deesterified	576:587	arg1	rhamnogalacturonan					589:606	deesterified rhamnogalacturonan I and 1,5-	576:617	deesterified rhamnogalacturonan I and 1,5-	576:617	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	5	24	theme	pectin	831:836	arg1	degradation					838:848	the pectin degradation	827:848	the pectin degradation	827:848	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	2	25	theme	FTIR	245:248	arg1	analyses					258:265	the FTIR and NMR analyses	241:265	analyses	258:265	The monosaccharide composition together with the FTIR and NMR analyses, indicated that both fractions are formed from a mixture of polysaccharides.					
30827593	3	26	theme	rhamnogalacturonan	417:434	arg1	blocks					438:443	rhamnogalacturonan I blocks	417:443	rhamnogalacturonan I blocks	417:443	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	4	27	theme	fraction	548:555	arg1	1A					557:558	The fraction 1A	544:558	The fraction 1A	544:558	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	4	27	theme	fraction	548:555	arg1	mixture					565:571	a mixture	563:571	a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides	563:675	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	3	28	theme	I	436:436	arg1	blocks					438:443	rhamnogalacturonan I blocks	417:443	rhamnogalacturonan I blocks	417:443	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	5	29	theme	heterogeneity	732:744	arg1	presence					682:689	The presence	678:689	The presence	678:689	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	3	30	theme	methyl-esterified	372:388	arg1	polysaccharide					397:410	methyl-esterified pectic polysaccharide	372:410	methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks	372:443	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	1	31	theme	alkali	146:151	arg1	1A					153:154	alkali 1A	146:154	alkali 1A	146:154	Two polysaccharide fractions sequentially extracted with water 1W and alkali 1A, were isolated from the hazelnut skins.					
30827593	2	32	theme	monosaccharide	200:213	arg1	composition					215:225	The monosaccharide composition	196:225	The monosaccharide composition	196:225	The monosaccharide composition together with the FTIR and NMR analyses, indicated that both fractions are formed from a mixture of polysaccharides.					
30827593	2	33	theme	polysaccharides	327:341	arg1	mixture					316:322	a mixture	314:322	a mixture of polysaccharides	314:341	The monosaccharide composition together with the FTIR and NMR analyses, indicated that both fractions are formed from a mixture of polysaccharides.					
30827593	5	34	gly	heterogeneity	732:744	arg1	weights					763:769	the molecular weights	749:769	the molecular weights	749:769	The presence of unsaturated galacturonic acid and the heterogeneity of the molecular weights, which Mw ranged between 3.6 and 39 kg mol-1, indicated the pectin degradation during roasting.					
30827593	3	35	theme	side	471:474	arg1	chains					476:481	arabinose side chains	461:481	arabinose side chains	461:481	The fraction 1W consists of methyl-esterified pectic polysaccharide with rhamnogalacturonan I blocks, branching with arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.					
30827593	4	36	theme	polysaccharides	661:675	arg1	1A					557:558	The fraction 1A	544:558	The fraction 1A	544:558	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	4	36	theme	polysaccharides	661:675	arg1	mixture					565:571	a mixture	563:571	a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides	563:675	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
30827593	2	37	theme	NMR	254:256	arg1	analyses					258:265	the FTIR and NMR analyses	241:265	analyses	258:265	The monosaccharide composition together with the FTIR and NMR analyses, indicated that both fractions are formed from a mixture of polysaccharides.					
30827593	4	38	theme	4-O-Me-glucuronoxylan	639:659	arg1	polysaccharides					661:675	4-O-Me-glucuronoxylan polysaccharides	639:675	4-O-Me-glucuronoxylan polysaccharides	639:675	The fraction 1A is a mixture of deesterified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.					
29474426	5	0	theme	transglycosylation	965:982	arg1	efficiencies					984:995	slightly reduced transglycosylation efficiencies	948:995	slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h	948:1053	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	1	1	theme	antibodies	278:287	arg1	properties					264:273	physicochemical properties	248:273	physicochemical properties	248:273	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	1	1	theme	antibodies	278:287	arg1	functions					234:242	biological functions	223:242	biological functions	223:242	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	5	2	theme	several	749:755	arg1	mutations					763:771	several other mutations	749:771	several other mutations	749:771	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	1	3	theme	fine	179:182	arg1	structures					184:193	The fine structures	175:193	The fine structures of Fc N-glycan	175:208	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	4	4	theme	improved	619:626	arg1	mutants					628:634	improved mutants	619:634	improved mutants of Endo-S	619:644	In this study, we generated improved mutants of Endo-S by introducing additional mutations to the D233Q mutant.					
29474426	7	5	theme	glycosylation	1310:1322	arg1	remodeling					1324:1333	glycosylation remodeling	1310:1333	glycosylation remodeling of antibodies	1310:1347	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	7	6	from	synthesis	1371:1379	arg1	advance					1397:1403	advance	1397:1403	advance	1397:1403	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	7	6	from	synthesis	1371:1379	arg1	situ					1411:1414	situ	1411:1414	situ	1411:1414	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	5	7	theme	reduced	957:963	arg1	efficiencies					984:995	slightly reduced transglycosylation efficiencies	948:995	slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h	948:1053	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	4	8	theme	Endo-S	639:644	arg1	mutants					628:634	improved mutants	619:634	improved mutants of Endo-S	619:644	In this study, we generated improved mutants of Endo-S by introducing additional mutations to the D233Q mutant.					
29474426	7	9	theme	chemical	1362:1369	arg1	synthesis					1371:1379	the chemical synthesis	1358:1379	the chemical synthesis of oxazoline in advance or in situ	1358:1414	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	5	10	theme	detectable	1015:1024	arg1	activity					1037:1044	no detectable hydrolysis activity	1012:1044	no detectable hydrolysis activity for 48 h	1012:1053	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	0	11	theme	one-pot	112:118	arg1	reaction					139:146	a novel one-pot transglycosylation reaction	104:146	a novel one-pot transglycosylation reaction	104:146	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	6	12	theme	one-pot	1217:1223	arg1	transglycosylation					1225:1242	one-pot transglycosylation	1217:1242	one-pot transglycosylation from sialoglycopeptide to antibodies	1217:1279	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	5	13	theme	other	920:924	arg1	mutations					926:934	several other mutations	912:934	several other mutations	912:934	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	0	14	theme	novel	106:110	arg1	reaction					139:146	a novel one-pot transglycosylation reaction	104:146	a novel one-pot transglycosylation reaction	104:146	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	6	15	theme	combined	1089:1096	arg1	use					1098:1100	the combined use	1085:1100	the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea,	1085:1207	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	16	theme	mutants	1105:1111	arg1	use					1098:1100	the combined use	1085:1100	the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea,	1085:1207	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	17	theme	Mucor	1169:1173	arg1	hiemalis					1175:1182	Mucor hiemalis	1169:1182	Mucor hiemalis	1169:1182	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	5	18	theme	transglycosylation	785:802	arg1	efficiencies					804:815	transglycosylation efficiencies	785:815	transglycosylation efficiencies exceeding 90%	785:829	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	6	19	gly	sialoglycopeptide	1249:1265	arg2	sialoglycopeptide					1249:1265	sialoglycopeptide	1249:1265	sialoglycopeptide	1249:1265	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	7	20	theme	antibodies	1338:1347	arg1	remodeling					1324:1333	glycosylation remodeling	1310:1333	glycosylation remodeling of antibodies	1310:1347	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	5	21	theme	hydrolysis	1026:1035	arg1	activity					1037:1044	no detectable hydrolysis activity	1012:1044	no detectable hydrolysis activity for 48 h	1012:1053	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	1	22	theme	Fc	198:199	arg1	N-glycan					201:208	Fc N-glycan	198:208	Fc N-glycan	198:208	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	3	23	theme	Such	434:437	arg1	remodeling					439:448	Such remodeling	434:448	Such remodeling	434:448	Such remodeling can be achieved by transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline.					
29474426	5	24	theme	other	757:761	arg1	mutations					763:771	several other mutations	749:771	several other mutations	749:771	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	1	25	theme	N-glycan	201:208	arg1	structures					184:193	The fine structures	175:193	The fine structures of Fc N-glycan	175:208	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	0	26	theme	mutants	24:30	arg1	Generation					0:9	Generation	0:9	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.	0:173	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	27	theme	efficient	14:22	arg1	mutants					24:30	efficient mutants	14:30	efficient mutants of endoglycosidase	14:49	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	28	theme	transglycosylation	120:137	arg1	reaction					139:146	a novel one-pot transglycosylation reaction	104:146	a novel one-pot transglycosylation reaction	104:146	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	29	from	application	89:99	arg1	Generation					0:9	Generation	0:9	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.	0:173	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	29	from	application	89:99	arg1	reaction					139:146	a novel one-pot transglycosylation reaction	104:146	a novel one-pot transglycosylation reaction	104:146	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	2	30	theme	homogeneous	325:335	arg1	glycoform					337:345	a homogeneous glycoform	323:345	a homogeneous glycoform	323:345	By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or modified.					
29474426	6	31	from	sialoglycopeptide	1249:1265	arg1	transglycosylation					1225:1242	one-pot transglycosylation	1217:1242	one-pot transglycosylation from sialoglycopeptide to antibodies	1217:1279	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	3	32	theme	endoglycosidase	516:530	arg1	mutant					506:511	a mutant	504:511	a mutant of endoglycosidase	504:530	Such remodeling can be achieved by transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline.					
29474426	0	33	theme	endoglycosidase	35:49	arg1	mutants					24:30	efficient mutants	14:30	efficient mutants of endoglycosidase	14:49	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	34	theme	antibody	152:159	arg1	modification					161:172	antibody modification	152:172	antibody modification	152:172	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	7	35	theme	novel	1287:1291	arg1	reaction					1293:1300	This novel reaction	1282:1300	This novel reaction	1282:1300	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	3	36	theme	transglycosylation	469:486	arg1	reactions					488:496	transglycosylation reactions	469:496	transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline	469:588	Such remodeling can be achieved by transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline.					
29474426	1	37	theme	biological	223:232	arg1	functions					234:242	biological functions	223:242	biological functions	223:242	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	5	38	theme	five	880:883	arg1	ratio					871:875	a single-digit donor-to-substrate ratio	837:875	a single-digit donor-to-substrate ratio of five	837:883	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	4	39	theme	D233Q	689:693	arg1	mutant					695:700	the D233Q mutant	685:700	the D233Q mutant	685:700	In this study, we generated improved mutants of Endo-S by introducing additional mutations to the D233Q mutant.					
29474426	0	40	theme	Streptococcus	56:68	arg1	pyogenes					70:77	Streptococcus pyogenes	56:77	Streptococcus pyogenes	56:77	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	6	41	theme	Endo-S	1116:1121	arg1	endoglycosidases					1147:1162	endoglycosidases	1147:1162	endoglycosidases from Mucor hiemalis and Coprinopsis cinerea	1147:1206	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	41	theme	Endo-S	1116:1121	arg1	mutants					1105:1111	mutants	1105:1111	mutants of Endo-S with Endo-M or Endo-CC	1105:1144	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	0	42	from	pyogenes	70:77	arg1	Generation					0:9	Generation	0:9	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.	0:173	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	0	42	from	pyogenes	70:77	arg1	reaction					139:146	a novel one-pot transglycosylation reaction	104:146	a novel one-pot transglycosylation reaction	104:146	Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification.					
29474426	6	43	from	cinerea	1200:1206	arg1	mutants					1105:1111	mutants	1105:1111	mutants of Endo-S with Endo-M or Endo-CC	1105:1144	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	43	from	cinerea	1200:1206	arg1	endoglycosidases					1147:1162	endoglycosidases	1147:1162	endoglycosidases from Mucor hiemalis and Coprinopsis cinerea	1147:1206	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	2	44	theme	antibody	378:385	arg1	characteristics					387:401	antibody characteristics	378:401	antibody characteristics	378:401	By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or modified.					
29474426	5	45	theme	donor-to-substrate	852:869	arg1	ratio					871:875	a single-digit donor-to-substrate ratio	837:875	a single-digit donor-to-substrate ratio of five	837:883	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	6	46	with	mutants	1105:1111	arg1	Endo-M					1128:1133	Endo-M	1128:1133	Endo-M	1128:1133	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	46	with	mutants	1105:1111	arg1	Endo-CC					1138:1144	Endo-CC	1138:1144	Endo-CC	1138:1144	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	2	47	theme	remodeling	293:302	arg1	N-glycan					304:311	remodeling N-glycan	293:311	remodeling N-glycan	293:311	By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or modified.					
29474426	7	48	theme	oxazoline	1384:1392	arg1	synthesis					1371:1379	the chemical synthesis	1358:1379	the chemical synthesis of oxazoline in advance or in situ	1358:1414	This novel reaction enables glycosylation remodeling of antibodies, without the chemical synthesis of oxazoline in advance or in situ.					
29474426	1	49	dep	functions	234:242	arg1	the					219:221	the	219:221	the	219:221	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	6	50	from	hiemalis	1175:1182	arg1	mutants					1105:1111	mutants	1105:1111	mutants of Endo-S with Endo-M or Endo-CC	1105:1144	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	6	50	from	hiemalis	1175:1182	arg1	endoglycosidases					1147:1162	endoglycosidases	1147:1162	endoglycosidases from Mucor hiemalis and Coprinopsis cinerea	1147:1206	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	2	51	theme	modified	361:368	arg1	glycan					370:375	chemically modified glycan	350:375	chemically modified glycan	350:375	By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or modified.					
29474426	1	52	theme	physicochemical	248:262	arg1	properties					264:273	physicochemical properties	248:273	physicochemical properties	248:273	The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies.					
29474426	5	53	theme	several	912:918	arg1	mutations					926:934	several other mutations	912:934	several other mutations	912:934	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	6	54	theme	Coprinopsis	1188:1198	arg1	cinerea					1200:1206	Coprinopsis cinerea	1188:1206	Coprinopsis cinerea	1188:1206	We further demonstrated that the combined use of mutants of Endo-S with Endo-M or Endo-CC, endoglycosidases from Mucor hiemalis and Coprinopsis cinerea, enables one-pot transglycosylation from sialoglycopeptide to antibodies.					
29474426	5	55	theme	single-digit	839:850	arg1	ratio					871:875	a single-digit donor-to-substrate ratio	837:875	a single-digit donor-to-substrate ratio of five	837:883	Notably, Endo-S D233Q/Q303L, D233Q/E350Q, and several other mutations resulted in transglycosylation efficiencies exceeding 90%, with a single-digit donor-to-substrate ratio of five, and D233Q/Y402F/D405A and several other mutations resulted in slightly reduced transglycosylation efficiencies accompanied by no detectable hydrolysis activity for 48 h.					
29474426	3	56	theme	glycan	573:578	arg1	oxazoline					580:588	glycan oxazoline	573:588	glycan oxazoline	573:588	Such remodeling can be achieved by transglycosylation reactions using a mutant of endoglycosidase from Streptococcus pyogenes (Endo-S) and glycan oxazoline.					
29474426	4	57	theme	additional	661:670	arg1	mutations					672:680	additional mutations	661:680	additional mutations	661:680	In this study, we generated improved mutants of Endo-S by introducing additional mutations to the D233Q mutant.					
31229065	5	0	dep	collapse	1482:1489	arg1	i.e.					1476:1479	i.e.	1476:1479	i.e.	1476:1479	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	1	theme	models	345:350	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	1	theme	models	345:350	arg1	transition					415:424	glass transition	409:424	glass transition	409:424	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	1	theme	models	345:350	arg1	transformation					249:262	structural transformation	238:262	structural transformation	238:262	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	1	theme	models	345:350	arg1	α-relaxation					431:442	α-relaxation	431:442	α-relaxation	431:442	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	1	theme	models	345:350	arg1	crystallization					392:406	time-dependent crystallization	377:406	time-dependent crystallization	377:406	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	1	theme	models	345:350	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	5	2	theme	models	1438:1443	arg1	transformation					1407:1420	structural transformation	1396:1420	structural transformation of honey powder models	1396:1443	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	3	theme	models	529:534	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	3	theme	models	529:534	arg1	transition					415:424	glass transition	409:424	glass transition	409:424	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	3	theme	models	529:534	arg1	transformation					249:262	structural transformation	238:262	structural transformation	238:262	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	3	theme	models	529:534	arg1	α-relaxation					431:442	α-relaxation	431:442	α-relaxation	431:442	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	3	theme	models	529:534	arg1	crystallization					392:406	time-dependent crystallization	377:406	time-dependent crystallization	377:406	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	3	theme	models	529:534	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	4	theme	crystallization	816:830	arg1	extent					806:811	the extent	802:811	the extent of crystallization and structural collapse	802:854	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	5	theme	MD	882:883	arg1	addition					885:892	MD addition	882:892	MD addition	882:892	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	0	6	theme	strength	78:85	arg1	analysis					87:94	strength analysis	78:94	strength analysis for honey powder models	78:118	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	5	7	dep	parameters	1347:1356	arg1	S					1377:1377	S	1377:1377	S	1377:1377	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	7	dep	parameters	1347:1356	arg1	parameters					1347:1356	the critical parameters	1334:1356	the critical parameters (water content and S)	1334:1378	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	7	dep	parameters	1347:1356	arg1	content					1365:1371	water content	1359:1371	water content	1359:1371	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	8	theme	various	447:453	arg1	activities					461:470	various water activities	447:470	various water activities (0.11aw to 0.76aw)	447:489	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	8	theme	various	447:453	arg1	0.76aw					483:488	0.11aw to 0.76aw	473:488	0.11aw to 0.76aw	473:488	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	9	theme	higher	622:627	arg1	content					645:651	higher monolayer water content	622:651	higher monolayer water content	622:651	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	10	theme	freeze-dried	267:278	arg1	H/WPI					308:312	H/WPI	308:312	H/WPI	308:312	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	10	theme	freeze-dried	267:278	arg1	isolate					299:305	freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models	267:350	isolate	299:305	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	0	11	theme	honey	100:104	arg1	models					113:118	honey powder models	100:118	honey powder models	100:118	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	1	12	theme	water	455:459	arg1	activities					461:470	various water activities	447:470	various water activities (0.11aw to 0.76aw)	447:489	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	12	theme	water	455:459	arg1	0.76aw					483:488	0.11aw to 0.76aw	473:488	0.11aw to 0.76aw	473:488	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	13	theme	monolayer	629:637	arg1	content					645:651	higher monolayer water content	622:651	higher monolayer water content	622:651	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	14	theme	honey/whey	280:289	arg1	H/WPI					308:312	H/WPI	308:312	H/WPI	308:312	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	14	theme	honey/whey	280:289	arg1	isolate					299:305	freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models	267:350	isolate	299:305	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	15	theme	water	361:365	arg1	sorption					367:374	water sorption	361:374	water sorption	361:374	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	16	theme	drying-aids	707:717	arg1	content					719:725	drying-aids content	707:725	drying-aids content	707:725	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	4	17	dep	H/WPI	1140:1144	arg1	models					1155:1160	models	1155:1160	models	1155:1160	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	2	18	theme	water	755:759	arg1	activity					761:768	water activity	755:768	water activity above 0.44aw and 25 °C	755:791	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	1	19	theme	protein	291:297	arg1	H/WPI					308:312	H/WPI	308:312	H/WPI	308:312	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	19	theme	protein	291:297	arg1	isolate					299:305	freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models	267:350	isolate	299:305	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	4	20	theme	strength	1106:1113	arg1	analysis					1115:1122	strength analysis	1106:1122	strength analysis of τ	1106:1127	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	3	21	theme	water	1064:1068	arg1	activity					1070:1077	water activity	1064:1077	water activity below 0.44aw	1064:1090	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	5	22	theme	structural	1396:1405	arg1	transformation					1407:1420	structural transformation	1396:1420	structural transformation of honey powder models	1396:1443	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	23	theme	S-involved	1280:1289	arg1	diagram					1297:1303	a S-involved state diagram	1278:1303	a S-involved state diagram	1278:1303	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	24	theme	relaxation	169:178	arg1	τ					186:186	τ	186:186	τ	186:186	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	24	theme	relaxation	169:178	arg1	time					180:183	relaxation time	169:183	relaxation time (τ)	169:187	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	0	25	theme	powder	106:111	arg1	models					113:118	honey powder models	100:118	honey powder models	100:118	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	1	26	theme	0.11aw	473:478	arg1	activities					461:470	various water activities	447:470	various water activities (0.11aw to 0.76aw)	447:489	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	26	theme	0.11aw	473:478	arg1	0.76aw					483:488	0.11aw to 0.76aw	473:488	0.11aw to 0.76aw	473:488	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	27	with	models	695:700	arg1	content					719:725	drying-aids content	707:725	drying-aids content	707:725	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	28	theme	ratios	745:750	arg1	%					735:735	50%	733:735	50% of mass ratios at water activity above 0.44aw and 25 °C	733:791	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	28	theme	ratios	745:750	arg1	ratios					745:750	mass ratios	740:750	mass ratios	740:750	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	1	29	theme	water	639:643	arg1	content					645:651	higher monolayer water content	622:651	higher monolayer water content	622:651	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	30	theme	time-dependent	377:390	arg1	crystallization					392:406	time-dependent crystallization	377:406	time-dependent crystallization	377:406	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	5	31	theme	honey	1425:1429	arg1	models					1438:1443	honey powder models	1425:1443	honey powder models	1425:1443	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	2	32	theme	sorption	903:910	arg1	isotherms					912:920	sorption isotherms	903:920	sorption isotherms	903:920	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	33	theme	mass	740:743	arg1	ratios					745:750	mass ratios	740:750	mass ratios	740:750	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	0	34	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	1	35	theme	isolate	299:305	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	35	theme	isolate	299:305	arg1	transition					415:424	glass transition	409:424	glass transition	409:424	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	35	theme	isolate	299:305	arg1	transformation					249:262	structural transformation	238:262	structural transformation	238:262	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	35	theme	isolate	299:305	arg1	α-relaxation					431:442	α-relaxation	431:442	α-relaxation	431:442	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	35	theme	isolate	299:305	arg1	crystallization					392:406	time-dependent crystallization	377:406	time-dependent crystallization	377:406	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	35	theme	isolate	299:305	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	5	36	theme	powder	1431:1436	arg1	models					1438:1443	honey powder models	1425:1443	honey powder models	1425:1443	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	37	theme	more	556:559	arg1	WPI					546:548	WPI	546:548	WPI	546:548	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	37	theme	more	556:559	arg1	stabilizer					582:591	a more effectiveness drying stabilizer	554:591	a more effectiveness drying stabilizer	554:591	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	0	38	theme	structural	28:37	arg1	transformation					39:52	structural transformation	28:52	structural transformation	28:52	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	1	39	theme	effectiveness	561:573	arg1	WPI					546:548	WPI	546:548	WPI	546:548	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	39	theme	effectiveness	561:573	arg1	stabilizer					582:591	a more effectiveness drying stabilizer	554:591	a more effectiveness drying stabilizer	554:591	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	40	located	observed	674:681	arg1	models					695:700	prepared models	686:700	prepared models with drying-aids content	686:725	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	40	located	observed	674:681	arg2	Crystallization					654:668	Crystallization	654:668	Crystallization	654:668	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	4	41	from	effects	1254:1260	arg1	τ					1265:1265	τ	1265:1265	τ	1265:1265	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	4	42	theme	compositional	1240:1252	arg1	effects					1254:1260	compositional effects	1240:1260	compositional effects on τ	1240:1265	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	1	43	theme	drying	575:580	arg1	WPI					546:548	WPI	546:548	WPI	546:548	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	43	theme	drying	575:580	arg1	stabilizer					582:591	a more effectiveness drying stabilizer	554:591	a more effectiveness drying stabilizer	554:591	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	3	44	theme	Glass	923:927	arg1	temperature					940:950	Glass transition temperature	923:950	Glass transition temperature	923:950	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	3	45	from	activity	1070:1077	arg1	WPI					1049:1051	WPI	1049:1051	WPI	1049:1051	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	3	45	from	activity	1070:1077	arg1	MD					1058:1059	MD	1058:1059	MD	1058:1059	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	3	45	from	activity	1070:1077	arg1	water					1042:1046	water	1042:1046	water	1042:1046	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	5	46	theme	critical	1338:1345	arg1	S					1377:1377	S	1377:1377	S	1377:1377	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	46	theme	critical	1338:1345	arg1	parameters					1347:1356	the critical parameters	1334:1356	the critical parameters (water content and S)	1334:1378	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	46	theme	critical	1338:1345	arg1	content					1365:1371	water content	1359:1371	water content	1359:1371	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	1	47	theme	glass	409:413	arg1	transition					415:424	glass transition	409:424	glass transition	409:424	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	48	theme	collapse	847:854	arg1	extent					806:811	the extent	802:811	the extent of crystallization and structural collapse	802:854	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	3	49	theme	α-relaxation	953:964	arg1	temperature					966:976	α-relaxation temperature	953:976	α-relaxation temperature	953:976	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	1	50	theme	physicochemical	207:221	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	0	51	theme	relaxation	59:68	arg1	time					70:73	relaxation time	59:73	relaxation time	59:73	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	1	52	theme	honey/maltodextrin	319:336	arg1	models					345:350	freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models	267:350	models	345:350	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	52	theme	honey/maltodextrin	319:336	arg1	H/MD					339:342	H/MD	339:342	H/MD	339:342	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	53	theme	25 °C.	495:500	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	4	54	theme	quantitative	1207:1218	arg1	measure					1220:1226	a quantitative measure	1205:1226	a quantitative measure to estimate compositional effects on τ	1205:1265	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	1	55	theme	Water	502:506	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	56	theme	Present	121:127	arg1	study					129:133	Present study	121:133	Present study	121:133	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	57	dep	observed	674:681	arg1	whereas					794:800	whereas	794:800	whereas	794:800	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	58	theme	prepared	686:693	arg1	models					695:700	prepared models	686:700	prepared models with drying-aids content	686:725	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	1	59	theme	sorption	508:515	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	5	60	theme	water	1359:1363	arg1	parameters					1347:1356	the critical parameters	1334:1356	the critical parameters (water content and S)	1334:1378	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	5	60	theme	water	1359:1363	arg1	content					1365:1371	water content	1359:1371	water content	1359:1371	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	2	61	from	activity	761:768	arg1	%					735:735	50%	733:735	50% of mass ratios at water activity above 0.44aw and 25 °C	733:791	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	2	61	from	activity	761:768	arg1	ratios					745:750	mass ratios	740:750	mass ratios	740:750	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
31229065	3	62	theme	transition	929:938	arg1	temperature					940:950	Glass transition temperature	923:950	Glass transition temperature	923:950	Glass transition temperature, α-relaxation temperature, and τ for two models were composition-dependent and altered by water, WPI, and MD at water activity below 0.44aw.					
31229065	5	63	theme	state	1291:1295	arg1	diagram					1297:1303	a S-involved state diagram	1278:1303	a S-involved state diagram	1278:1303	Moreover, a S-involved state diagram was established to determine the critical parameters (water content and S) for controlling structural transformation of honey powder models during production and storage, i.e., collapse and stickiness.					
31229065	0	64	dep	properties	16:25	arg1	analysis					87:94	strength analysis	78:94	strength analysis for honey powder models	78:118	Physicochemical properties, structural transformation, and relaxation time in strength analysis for honey powder models.					
31229065	4	65	theme	τ	1127:1127	arg1	analysis					1115:1122	strength analysis	1106:1122	strength analysis of τ	1106:1127	According to strength analysis of τ, the S for H/WPI and H/MD models was affected by drying-aids and could give a quantitative measure to estimate compositional effects on τ.					
31229065	1	66	from	activities	461:470	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	66	from	activities	461:470	arg1	transition					415:424	glass transition	409:424	glass transition	409:424	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	66	from	activities	461:470	arg1	transformation					249:262	structural transformation	238:262	structural transformation	238:262	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	66	from	activities	461:470	arg1	α-relaxation					431:442	α-relaxation	431:442	α-relaxation	431:442	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	66	from	activities	461:470	arg1	crystallization					392:406	time-dependent crystallization	377:406	time-dependent crystallization	377:406	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	66	from	activities	461:470	arg1	data					517:520	25 °C. Water sorption data	495:520	25 °C. Water sorption data	495:520	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	67	theme	H/WPI	604:608	arg1	model					610:614	H/WPI model	604:614	H/WPI model	604:614	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	68	theme	structural	238:247	arg1	transformation					249:262	structural transformation	238:262	structural transformation	238:262	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	69	theme	strength	147:154	arg1	analysis					156:163	a strength analysis	145:163	a strength analysis for relaxation time (τ)	145:187	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	1	70	dep	0.76aw	483:488	arg1	to					480:481	to	480:481	to	480:481	Present study developed a strength analysis for relaxation time (τ) in characterizing physicochemical properties and structural transformation of freeze-dried honey/whey protein isolate (H/WPI) and honey/maltodextrin (H/MD) models based on water sorption, time-dependent crystallization, glass transition, and α-relaxation at various water activities (0.11aw to 0.76aw) and 25 °C. Water sorption data of two models explained WPI was a more effectiveness drying stabilizer than MD as H/WPI model owned higher monolayer water content.					
31229065	2	71	theme	structural	836:845	arg1	collapse					847:854	structural collapse	836:854	structural collapse	836:854	Crystallization was observed in prepared models with drying-aids content below 50% of mass ratios at water activity above 0.44aw and 25 °C, whereas the extent of crystallization and structural collapse were inhibited by WPI and MD addition based on sorption isotherms.					
30446152	0	0	theme	oxygen	97:102	arg1	performance					112:122	high oxygen barrier performance	92:122	high oxygen barrier performance	92:122	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	4	1	theme	*	874:874	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	1	theme	*	874:874	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	2	2	theme	oxygen	416:421	arg1	property					431:438	excellent oxygen barrier property	406:438	excellent oxygen barrier property	406:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	6	3	theme	several	1141:1147	arg1	fields					1149:1154	several fields	1141:1154	several fields as packaging materials, such as drug, food and electronic products	1141:1221	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	0	4	theme	high	92:95	arg1	performance					112:122	high oxygen barrier performance	92:122	high oxygen barrier performance	92:122	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	3	5	theme	OTR	716:718	arg1	measurement					721:731	oxygen transmission rate (OTR) measurement	690:731	oxygen transmission rate (OTR) measurement	690:731	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	4	6	theme	m2	872:873	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	6	theme	m2	872:873	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	2	7	theme	excellent	406:414	arg1	property					431:438	excellent oxygen barrier property	406:438	excellent oxygen barrier property	406:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	5	8	theme	light	993:997	arg1	transmittance					999:1011	light transmittance	993:1011	light transmittance	993:1011	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	5	9	theme	hybrid	946:951	arg1	films					953:957	the hybrid films	942:957	the hybrid films	942:957	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	4	10	theme	*	876:876	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	10	theme	*	876:876	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	2	11	theme	acid-grafted-acryloyl/hemicellulose	325:359	arg1	materials					391:399	film materials	386:399	film materials with excellent oxygen barrier property	386:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	2	11	theme	acid-grafted-acryloyl/hemicellulose	325:359	arg1	PAA-g-AH					373:380	PAA-g-AH	373:380	PAA-g-AH	373:380	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	2	11	theme	acid-grafted-acryloyl/hemicellulose	325:359	arg1	composites					361:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites	313:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH)	313:381	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	3	12	theme	1H	609:610	arg1	resonance					629:637	1H nuclear magnetic resonance	609:637	1H nuclear magnetic resonance (1H NMR)	609:646	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	12	theme	1H	609:610	arg1	NMR					643:645	1H NMR	640:645	1H NMR	640:645	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	6	13	theme	electronic	1203:1212	arg1	products					1214:1221	electronic products	1203:1221	electronic products	1203:1221	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	13	theme	electronic	1203:1212	arg1	drug					1188:1191	drug	1188:1191	drug	1188:1191	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	13	theme	electronic	1203:1212	arg1	materials					1169:1177	packaging materials	1159:1177	packaging materials	1159:1177	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	0	14	theme	barrier	104:110	arg1	performance					112:122	high oxygen barrier performance	92:122	high oxygen barrier performance	92:122	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	4	15	theme	d	875:875	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	15	theme	d	875:875	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	2	16	theme	polyacrylic	313:323	arg1	materials					391:399	film materials	386:399	film materials with excellent oxygen barrier property	386:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	2	16	theme	polyacrylic	313:323	arg1	PAA-g-AH					373:380	PAA-g-AH	373:380	PAA-g-AH	373:380	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	2	16	theme	polyacrylic	313:323	arg1	composites					361:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites	313:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH)	313:381	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	4	17	theme	testing	744:750	arg1	results					752:758	All these testing results	734:758	All these testing results	734:758	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	18	theme	PAA	922:924	arg1	film					926:929	pure PAA film	917:929	pure PAA film	917:929	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	6	19	theme	great	1109:1113	arg1	applications					1125:1136	great potential applications	1109:1136	great potential applications	1109:1136	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	4	20	theme	*	866:866	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	20	theme	*	866:866	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	3	21	theme	scanning	536:543	arg1	SEM					566:568	SEM	566:568	SEM	566:568	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	21	theme	scanning	536:543	arg1	microscopy					554:563	scanning electron microscopy	536:563	scanning electron microscopy (SEM)	536:569	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	4	22	theme	oxygen	803:808	arg1	property					818:825	great oxygen barrier property	797:825	great oxygen barrier property	797:825	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	23	theme	low	834:836	arg1	value					842:846	a low OTR value	832:846	a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film	832:929	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	24	theme	pure	917:920	arg1	film					926:929	pure PAA film	917:929	pure PAA film	917:929	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	25	theme	0.25 ± 0.01 cm3	851:865	arg1	 μm/					867:870	0.25 ± 0.01 cm3* μm/	851:870	0.25 ± 0.01 cm3* μm/(m2*d*kPa)	851:880	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	25	theme	0.25 ± 0.01 cm3	851:865	arg1	kPa					877:879	m2*d*kPa	872:879	m2*d*kPa	872:879	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	26	theme	great	797:801	arg1	property					818:825	great oxygen barrier property	797:825	great oxygen barrier property	797:825	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	3	27	theme	as-synthesized	445:458	arg1	films					460:464	The as-synthesized films	441:464	The as-synthesized films	441:464	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	28	theme	permeation	576:585	arg1	GPC					603:605	GPC	603:605	GPC	603:605	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	28	theme	permeation	576:585	arg1	chromatography					587:600	gel permeation chromatography	572:600	gel permeation chromatography (GPC)	572:606	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	29	theme	nuclear	612:618	arg1	resonance					629:637	1H nuclear magnetic resonance	609:637	1H nuclear magnetic resonance (1H NMR)	609:646	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	29	theme	nuclear	612:618	arg1	NMR					643:645	1H NMR	640:645	1H NMR	640:645	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	4	30	theme	 μm/	867:870	arg1	value					842:846	a low OTR value	832:846	a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film	832:929	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	3	31	theme	magnetic	620:627	arg1	resonance					629:637	1H nuclear magnetic resonance	609:637	1H nuclear magnetic resonance (1H NMR)	609:646	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	31	theme	magnetic	620:627	arg1	NMR					643:645	1H NMR	640:645	1H NMR	640:645	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	0	32	theme	acid-grafted-acryloyl/hemicellulose	27:61	arg1	films					81:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	4	33	theme	OTR	838:840	arg1	value					842:846	a low OTR value	832:846	a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film	832:929	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	4	34	theme	optimum	776:782	arg1	film					784:787	the optimum film	772:787	the optimum film	772:787	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	3	35	dep	Fourier	500:506	arg1	transform					508:516	transform	508:516	transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement	508:731	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	36	theme	light	666:670	arg1	transmittance					672:684	light transmittance	666:684	light transmittance	666:684	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	0	37	theme	polyacrylic	15:25	arg1	films					81:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	5	38	theme	usage	1025:1029	arg1	properties					1031:1040	recycle usage properties	1017:1040	recycle usage properties	1017:1040	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	1	39	theme	Developing	125:134	arg1	films					168:172	Developing high-performance oxygen barrier films	125:172	Developing high-performance oxygen barrier films using biomass-based materials	125:202	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	1	40	theme	green	238:242	arg1	development					223:233	the development	219:233	the development of green and sustainable society	219:266	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	0	41	theme	PAA-g-AH	64:71	arg1	films					81:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	3	42	theme	1H	640:641	arg1	resonance					629:637	1H nuclear magnetic resonance	609:637	1H nuclear magnetic resonance (1H NMR)	609:646	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	42	theme	1H	640:641	arg1	NMR					643:645	1H NMR	640:645	1H NMR	640:645	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	6	43	theme	Experimental	1043:1054	arg1	results					1056:1062	Experimental results	1043:1062	Experimental results	1043:1062	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	44	contain	have	1104:1107	arg1	films					1098:1102	the as-synthesized films	1079:1102	the as-synthesized films	1079:1102	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	44	contain	have	1104:1107	arg2	applications					1125:1136	great potential applications	1109:1136	great potential applications	1109:1136	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	3	45	dep	transform	508:516	arg1	infrared					518:525	infrared	518:525	infrared	518:525	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	1	46	theme	sustainable	248:258	arg1	development					223:233	the development	219:233	the development of green and sustainable society	219:266	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	0	47	theme	hybrid	74:79	arg1	films					81:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films	15:85	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	2	48	with	materials	391:399	arg1	property					431:438	excellent oxygen barrier property	406:438	excellent oxygen barrier property	406:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	3	49	dep	infrared	518:525	arg1	FT-IR					528:532	FT-IR	528:532	FT-IR	528:532	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	1	50	theme	high-performance	136:151	arg1	films					168:172	Developing high-performance oxygen barrier films	125:172	Developing high-performance oxygen barrier films using biomass-based materials	125:202	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	6	51	theme	packaging	1159:1167	arg1	food					1194:1197	food	1194:1197	food	1194:1197	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	51	theme	packaging	1159:1167	arg1	products					1214:1221	electronic products	1203:1221	electronic products	1203:1221	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	51	theme	packaging	1159:1167	arg1	drug					1188:1191	drug	1188:1191	drug	1188:1191	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	6	51	theme	packaging	1159:1167	arg1	materials					1169:1177	packaging materials	1159:1177	packaging materials	1159:1177	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	3	52	theme	electron	545:552	arg1	SEM					566:568	SEM	566:568	SEM	566:568	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	52	theme	electron	545:552	arg1	microscopy					554:563	scanning electron microscopy	536:563	scanning electron microscopy (SEM)	536:569	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	53	theme	rate	710:713	arg1	measurement					721:731	oxygen transmission rate (OTR) measurement	690:731	oxygen transmission rate (OTR) measurement	690:731	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	1	54	theme	oxygen	153:158	arg1	films					168:172	Developing high-performance oxygen barrier films	125:172	Developing high-performance oxygen barrier films using biomass-based materials	125:202	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	5	55	theme	recycle	1017:1023	arg1	properties					1031:1040	recycle usage properties	1017:1040	recycle usage properties	1017:1040	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	5	56	theme	mechanical	972:981	arg1	strength					983:990	mechanical strength	972:990	mechanical strength	972:990	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	3	57	theme	mechanical	649:658	arg1	test					660:663	mechanical test	649:663	mechanical test	649:663	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	0	58	with	Preparation	0:10	arg1	performance					112:122	high oxygen barrier performance	92:122	high oxygen barrier performance	92:122	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	1	59	theme	barrier	160:166	arg1	films					168:172	Developing high-performance oxygen barrier films	125:172	Developing high-performance oxygen barrier films using biomass-based materials	125:202	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	6	60	theme	as-synthesized	1083:1096	arg1	films					1098:1102	the as-synthesized films	1079:1102	the as-synthesized films	1079:1102	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	3	61	theme	oxygen	690:695	arg1	measurement					721:731	oxygen transmission rate (OTR) measurement	690:731	oxygen transmission rate (OTR) measurement	690:731	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	4	62	theme	barrier	810:816	arg1	property					818:825	great oxygen barrier property	797:825	great oxygen barrier property	797:825	All these testing results showed that the optimum film exhibit great oxygen barrier property with a low OTR value of 0.25 ± 0.01 cm3* μm/(m2*d*kPa), which was much lower than that of pure PAA film.					
30446152	1	63	theme	biomass-based	180:192	arg1	materials					194:202	biomass-based materials	180:202	biomass-based materials	180:202	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	2	64	theme	film	386:389	arg1	materials					391:399	film materials	386:399	film materials with excellent oxygen barrier property	386:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	2	64	theme	film	386:389	arg1	composites					361:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites	313:370	polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH)	313:381	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	3	65	theme	transmission	697:708	arg1	measurement					721:731	oxygen transmission rate (OTR) measurement	690:731	oxygen transmission rate (OTR) measurement	690:731	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	66	theme	gel	572:574	arg1	GPC					603:605	GPC	603:605	GPC	603:605	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	3	66	theme	gel	572:574	arg1	chromatography					587:600	gel permeation chromatography	572:600	gel permeation chromatography (GPC)	572:606	The as-synthesized films were extensively characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), gel permeation chromatography (GPC), 1H nuclear magnetic resonance (1H NMR), mechanical test, light transmittance and oxygen transmission rate (OTR) measurement.					
30446152	0	67	theme	films	81:85	arg1	Preparation					0:10	Preparation	0:10	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.	0:123	Preparation of polyacrylic acid-grafted-acryloyl/hemicellulose (PAA-g-AH) hybrid films with high oxygen barrier performance.					
30446152	6	68	theme	potential	1115:1123	arg1	applications					1125:1136	great potential applications	1109:1136	great potential applications	1109:1136	Experimental results indicated that the as-synthesized films have great potential applications in several fields as packaging materials, such as drug, food and electronic products.					
30446152	1	69	dep	green	238:242	arg1	society					260:266	society	260:266	society	260:266	Developing high-performance oxygen barrier films using biomass-based materials is crucial for the development of green and sustainable society.					
30446152	2	70	theme	barrier	423:429	arg1	property					431:438	excellent oxygen barrier property	406:438	excellent oxygen barrier property	406:438	Herein, we develop a strategy to synthesize polyacrylic acid-grafted-acryloyl/hemicellulose composites (PAA-g-AH) as film materials with excellent oxygen barrier property.					
30446152	5	71	contain	possess	959:965	arg1	films					953:957	the hybrid films	942:957	the hybrid films	942:957	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	5	71	contain	possess	959:965	arg2	properties					1031:1040	recycle usage properties	1017:1040	recycle usage properties	1017:1040	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	5	71	contain	possess	959:965	arg2	strength					983:990	mechanical strength	972:990	mechanical strength	972:990	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30446152	5	71	contain	possess	959:965	arg2	transmittance					999:1011	light transmittance	993:1011	light transmittance	993:1011	Moreover, the hybrid films possess well mechanical strength, light transmittance and recycle usage properties.					
30007628	0	0	theme	analysis	89:96	arg1	pectins					126:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins	51:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	51:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	9	1	attach	presence	1003:1010	arg2	galactan					1024:1031	a linear galactan	1015:1031	a linear galactan	1015:1031	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	9	1	attach	presence	1003:1010	arg1	form					1041:1044	free form	1036:1044	free form in fruits	1036:1054	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	2	2	theme	hydroxide	334:342	arg1	solutions					344:352	sodium hydroxide solutions	327:352	sodium hydroxide solutions	327:352	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	0	3	theme	structural	78:87	arg1	analysis					89:96	structural analysis	78:96	structural analysis of a galactan-rich fraction	78:124	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	8	4	theme	17,560 g/mol	953:964	arg1	mass					945:948	molar mass	939:948	molar mass of 17,560 g/mol	939:964	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	2	5	theme	sodium	327:332	arg1	hydroxide					334:342	sodium hydroxide	327:342	sodium hydroxide solutions	327:352	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	7	6	theme	linear	837:842	arg1	SCAI2					854:858	SCAI2	854:858	SCAI2	854:858	The SCA fraction was further purified, resulting in a linear galactan (SCAI2).					
30007628	7	6	theme	linear	837:842	arg1	galactan					844:851	a linear galactan	835:851	a linear galactan (SCAI2)	835:859	The SCA fraction was further purified, resulting in a linear galactan (SCAI2).					
30007628	2	7	with	extraction	287:296	arg1	solutions					344:352	sodium hydroxide solutions	327:352	sodium hydroxide solutions	327:352	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	2	7	with	extraction	287:296	arg1	water					303:307	water	303:307	water	303:307	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	2	7	with	extraction	287:296	arg1	acid					317:320	citric acid	310:320	citric acid	310:320	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	0	8	from	Characterization	0:15	arg1	pectins					126:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins	51:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	51:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	2	9	theme	sequential	276:285	arg1	extraction					287:296	sequential extraction	276:296	sequential extraction with water, citric acid, and sodium hydroxide solutions	276:352	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	9	10	theme	side	1107:1110	arg1	chains					1112:1117	side chains	1107:1117	side chains of type I rhamnogalacturonans	1107:1147	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	9	11	theme	free	1036:1039	arg1	form					1041:1044	free form	1036:1044	free form in fruits	1036:1054	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	0	12	theme	fraction	117:124	arg1	fruit					68:72	Sicana odorifera fruit	51:72	Sicana odorifera fruit	51:72	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	0	12	theme	fraction	117:124	arg1	analysis					89:96	structural analysis	78:96	structural analysis of a galactan-rich fraction	78:124	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	6	13	theme	mannans	774:780	arg1	presence					738:745	the presence	734:745	the presence of xyloglucans, xylans and mannans	734:780	The hemicellulosic fractions consisted mainly of xylose, mannose, galactose, and glucose, suggesting the presence of xyloglucans, xylans and mannans.					
30007628	9	14	from	form	1041:1044	arg1	fruits					1049:1054	fruits	1049:1054	fruits	1049:1054	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	0	15	theme	galactan-rich	103:115	arg1	fraction					117:124	a galactan-rich fraction	101:124	a galactan-rich fraction	101:124	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	6	16	theme	xylans	763:768	arg1	presence					738:745	the presence	734:745	the presence of xyloglucans, xylans and mannans	734:780	The hemicellulosic fractions consisted mainly of xylose, mannose, galactose, and glucose, suggesting the presence of xyloglucans, xylans and mannans.					
30007628	5	17	theme	citric	569:574	arg1	fraction					581:588	The citric acid fraction	565:588	The citric acid fraction (SCA)	565:594	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	17	theme	citric	569:574	arg1	SCA					591:593	SCA	591:593	SCA	591:593	The citric acid fraction (SCA) had galactose as the main component.					
30007628	1	18	theme	Brazilian	172:180	arg1	fruit					189:193	a Brazilian native fruit	170:193	a Brazilian native fruit	170:193	Sicana odorifera is a Brazilian native fruit.					
30007628	1	18	theme	Brazilian	172:180	arg1	odorifera					157:165	Sicana odorifera	150:165	Sicana odorifera	150:165	Sicana odorifera is a Brazilian native fruit.					
30007628	5	19	theme	main	617:620	arg1	component					622:630	the main component	613:630	the main component	613:630	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	19	theme	main	617:620	arg1	galactose					600:608	galactose	600:608	galactose	600:608	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	20	theme	acid	576:579	arg1	fraction					581:588	The citric acid fraction	565:588	The citric acid fraction (SCA)	565:594	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	20	theme	acid	576:579	arg1	SCA					591:593	SCA	591:593	SCA	591:593	The citric acid fraction (SCA) had galactose as the main component.					
30007628	3	21	theme	polysaccharide	395:408	arg1	fractions					410:418	crude polysaccharide fractions	389:418	crude polysaccharide fractions	389:418	The monosaccharide composition of crude polysaccharide fractions was determined.					
30007628	9	22	theme	linear	1017:1022	arg1	galactan					1024:1031	a linear galactan	1015:1031	a linear galactan	1015:1031	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	1	23	theme	native	182:187	arg1	fruit					189:193	a Brazilian native fruit	170:193	a Brazilian native fruit	170:193	Sicana odorifera is a Brazilian native fruit.					
30007628	1	23	theme	native	182:187	arg1	odorifera					157:165	Sicana odorifera	150:165	Sicana odorifera	150:165	Sicana odorifera is a Brazilian native fruit.					
30007628	9	24	theme	I	1127:1127	arg1	rhamnogalacturonans					1129:1147	type I rhamnogalacturonans	1122:1147	type I rhamnogalacturonans	1122:1147	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	9	25	theme	galactan	1024:1031	arg1	presence					1003:1010	The presence	999:1010	The presence of a linear galactan in free form in fruits	999:1054	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	9	25	theme	galactan	1024:1031	arg1	unusual					1059:1065	unusual	1059:1065	unusual	1059:1065	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	0	26	theme	wall	25:28	arg1	polysaccharides					30:44	cell wall polysaccharides	20:44	cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	20:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	4	27	theme	side	552:555	arg1	chains					557:562	side chains	552:562	side chains	552:562	The aqueous fractions displayed the highest yields and they were constituted by pectins, having mainly galactans as side chains.					
30007628	6	28	theme	xyloglucans	750:760	arg1	presence					738:745	the presence	734:745	the presence of xyloglucans, xylans and mannans	734:780	The hemicellulosic fractions consisted mainly of xylose, mannose, galactose, and glucose, suggesting the presence of xyloglucans, xylans and mannans.					
30007628	0	29	theme	cell	20:23	arg1	polysaccharides					30:44	cell wall polysaccharides	20:44	cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	20:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	2	30	from	pulp	254:257	arg1	polysaccharides					220:234	cell wall polysaccharides	210:234	cell wall polysaccharides from S. odorifera pulp	210:257	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	3	31	theme	fractions	410:418	arg1	composition					374:384	The monosaccharide composition	355:384	The monosaccharide composition of crude polysaccharide fractions	355:418	The monosaccharide composition of crude polysaccharide fractions was determined.					
30007628	8	32	theme	NMR	862:864	arg1	analysis					882:889	NMR and methylation analysis	862:889	analysis	882:889	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	0	33	theme	polysaccharides	30:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.	0:148	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	0	34	theme	side	137:140	arg1	chains					142:147	side chains	137:147	side chains	137:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	4	35	theme	highest	472:478	arg1	yields					480:485	the highest yields	468:485	the highest yields	468:485	The aqueous fractions displayed the highest yields and they were constituted by pectins, having mainly galactans as side chains.					
30007628	2	36	theme	wall	215:218	arg1	polysaccharides					220:234	cell wall polysaccharides	210:234	cell wall polysaccharides from S. odorifera pulp	210:257	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	3	37	theme	crude	389:393	arg1	fractions					410:418	crude polysaccharide fractions	389:418	crude polysaccharide fractions	389:418	The monosaccharide composition of crude polysaccharide fractions was determined.					
30007628	7	38	theme	SCA	787:789	arg1	fraction					791:798	The SCA fraction	783:798	The SCA fraction	783:798	The SCA fraction was further purified, resulting in a linear galactan (SCAI2).					
30007628	2	39	theme	cell	210:213	arg1	polysaccharides					220:234	cell wall polysaccharides	210:234	cell wall polysaccharides from S. odorifera pulp	210:257	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	8	40	theme	methylation	870:880	arg1	analysis					882:889	NMR and methylation analysis	862:889	analysis	882:889	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	0	41	theme	Sicana	51:56	arg1	fruit					68:72	Sicana odorifera fruit	51:72	Sicana odorifera fruit	51:72	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	8	42	theme	light	981:985	arg1	scattering					987:996	light scattering	981:996	light scattering	981:996	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	9	43	theme	rhamnogalacturonans	1129:1147	arg1	chains					1112:1117	side chains	1107:1117	side chains of type I rhamnogalacturonans	1107:1147	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	8	44	theme	molar	939:943	arg1	mass					945:948	molar mass	939:948	molar mass of 17,560 g/mol	939:964	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	9	45	from	presence	1003:1010	arg1	form					1041:1044	free form	1036:1044	free form in fruits	1036:1054	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	0	46	dep	Sicana	51:56	arg1	odorifera					58:66	odorifera	58:66	odorifera	58:66	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	6	47	theme	hemicellulosic	637:650	arg1	fractions					652:660	The hemicellulosic fractions	633:660	The hemicellulosic fractions	633:660	The hemicellulosic fractions consisted mainly of xylose, mannose, galactose, and glucose, suggesting the presence of xyloglucans, xylans and mannans.					
30007628	4	48	theme	aqueous	440:446	arg1	fractions					448:456	The aqueous fractions	436:456	The aqueous fractions	436:456	The aqueous fractions displayed the highest yields and they were constituted by pectins, having mainly galactans as side chains.					
30007628	2	49	theme	odorifera	244:252	arg1	pulp					254:257	S. odorifera pulp	241:257	S. odorifera pulp	241:257	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	2	50	theme	citric	310:315	arg1	acid					317:320	citric acid	310:320	citric acid	310:320	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	8	51	with	d-galactan	923:932	arg1	mass					945:948	molar mass	939:948	molar mass of 17,560 g/mol	939:964	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	2	52	theme	S.	241:242	arg1	pulp					254:257	S. odorifera pulp	241:257	S. odorifera pulp	241:257	In this work, cell wall polysaccharides from S. odorifera pulp were isolated by sequential extraction with water, citric acid, and sodium hydroxide solutions.					
30007628	5	53	contain	had	596:598	arg1	fraction					581:588	The citric acid fraction	565:588	The citric acid fraction (SCA)	565:594	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	53	contain	had	596:598	arg2	galactose					600:608	galactose	600:608	galactose	600:608	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	53	contain	had	596:598	arg2	component					622:630	the main component	613:630	the main component	613:630	The citric acid fraction (SCA) had galactose as the main component.					
30007628	5	53	contain	had	596:598	arg1	SCA					591:593	SCA	591:593	SCA	591:593	The citric acid fraction (SCA) had galactose as the main component.					
30007628	9	54	theme	type	1122:1125	arg1	rhamnogalacturonans					1129:1147	type I rhamnogalacturonans	1122:1147	type I rhamnogalacturonans	1122:1147	The presence of a linear galactan in free form in fruits is unusual because these polymers usually occur as side chains of type I rhamnogalacturonans.					
30007628	0	55	theme	fruit	68:72	arg1	pectins					126:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins	51:132	Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	51:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	3	56	theme	monosaccharide	359:372	arg1	composition					374:384	The monosaccharide composition	355:384	The monosaccharide composition of crude polysaccharide fractions	355:418	The monosaccharide composition of crude polysaccharide fractions was determined.					
30007628	8	57	theme	β-	915:916	arg1	d-galactan					923:932	a β-(1→4) d-galactan	913:932	a β-(1→4) d-galactan	913:932	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	8	57	theme	β-	915:916	arg1	SCAI2					903:907	SCAI2	903:907	SCAI2	903:907	NMR and methylation analysis showed that SCAI2 was a β-(1→4) d-galactan with molar mass of 17,560 g/mol, determined by light scattering.					
30007628	1	58	theme	Sicana	150:155	arg1	fruit					189:193	a Brazilian native fruit	170:193	a Brazilian native fruit	170:193	Sicana odorifera is a Brazilian native fruit.					
30007628	1	58	theme	Sicana	150:155	arg1	odorifera					157:165	Sicana odorifera	150:165	Sicana odorifera	150:165	Sicana odorifera is a Brazilian native fruit.					
30007628	0	59	from	pectins	126:132	arg1	Characterization					0:15	Characterization	0:15	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.	0:148	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
30007628	0	59	from	pectins	126:132	arg1	polysaccharides					30:44	cell wall polysaccharides	20:44	cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains	20:147	Characterization of cell wall polysaccharides from Sicana odorifera fruit and structural analysis of a galactan-rich fraction pectins as side chains.					
29767770	2	0	theme	terminal	590:597	arg1	units					615:619	terminal nonreducing end units	590:619	terminal nonreducing end units	590:619	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	1	contain	containing	444:453	arg2	residues					488:495	(1→6)-linked β-D-galactofuranose residues	455:495	(1→6)-linked β-D-galactofuranose residues	455:495	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	1	contain	containing	444:453	arg1	chain					438:442	a main chain	431:442	a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2)	431:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	8	2	theme	PGM	1448:1450	arg1	motifs					1452:1457	another PGM motifs	1440:1457	another PGM motifs able to induce a higher secretion of TNF-α levels	1440:1507	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	1	3	theme	chemical	277:284	arg1	methods					304:310	chemical and spectroscopic methods	277:310	chemical and spectroscopic methods	277:310	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	3	theme	chemical	277:284	arg1	one					323:325	one	323:325	one	323:325	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	3	theme	chemical	277:284	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	2	4	theme	units	803:807	arg1	β-D-Galf					772:779	β-D-Galf	772:779	β-D-Galf	772:779	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	4	theme	units	803:807	arg1	chains					789:794	side chains	784:794	side chains of 3-4 units that are (1→5)-interlinked	784:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	1	5	theme	spectroscopic	290:302	arg1	methods					304:310	chemical and spectroscopic methods	277:310	chemical and spectroscopic methods	277:310	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	5	theme	spectroscopic	290:302	arg1	one					323:325	one	323:325	one	323:325	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	5	theme	spectroscopic	290:302	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	0	6	link	β-1,6-linked	0:11	arg1	peptidogalactomannan					35:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan	0:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum	0:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	2	7	theme	-linked	735:741	arg1	residues					752:759	(1→6)-linked α-D-Manp residues	730:759	(1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	730:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	10	8	theme	diagnostic	1887:1896	arg1	antigen					1898:1904	a potential diagnostic antigen	1875:1904	a potential diagnostic antigen	1875:1904	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	10	9	theme	present	1715:1721	arg1	study					1723:1727	the present study	1711:1727	the present study	1711:1727	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	2	10	theme	end	611:613	arg1	units					615:619	terminal nonreducing end units	590:619	terminal nonreducing end units	590:619	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	5	11	from	decrease	996:1003	arg1	reactivity					1008:1017	reactivity	1008:1017	reactivity	1008:1017	A strong decrease in reactivity was also observed with de-O-glycosylated PGM.					
29767770	2	12	link	-linked	553:559	arg1	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	12	link	-linked	553:559	arg1	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	12	link	-linked	553:559	arg1	1→2					572:574	1→2	572:574	1→2	572:574	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	0	13	theme	potential	143:151	arg1	antigen					164:170	a potential diagnostic antigen	141:170	a potential diagnostic antigen	141:170	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	0	14	from	important	81:89	arg1	activation					98:107	the activation	94:107	the activation of macrophage mechanisms	94:132	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	8	15	theme	able	1459:1462	arg1	motifs					1452:1457	another PGM motifs	1440:1457	another PGM motifs able to induce a higher secretion of TNF-α levels	1440:1507	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	9	16	dep	F.	1525:1526	arg1	conidia					1538:1544	F. oxysporum conidia, intact and de-O-linked PGM	1525:1572	conidia	1538:1544	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	9	16	dep	F.	1525:1526	arg1	PGM					1570:1572	intact and de-O-linked PGM	1547:1572	intact and de-O-linked PGM	1547:1572	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	10	17	dep	F.	1681:1682	arg1	oxysporum					1684:1692	F. oxysporum	1681:1692	F. oxysporum	1681:1692	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	10	18	theme	same	1787:1790	arg1	epitopes					1792:1799	the same epitopes	1783:1799	the same epitopes recognized by serum from patients with aspergillosis	1783:1852	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	8	19	theme	O-linked	1304:1311	arg1	oligosaccharides					1313:1328	O-linked oligosaccharides	1304:1328	O-linked oligosaccharides	1304:1328	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	0	20	dep	potential	143:151	arg1	diagnostic					153:162	diagnostic	153:162	diagnostic	153:162	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	8	21	theme	higher	1476:1481	arg1	secretion					1483:1491	a higher secretion	1474:1491	a higher secretion of TNF-α levels	1474:1507	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	2	22	link	-linked	530:536	arg1	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	22	link	-linked	530:536	arg1	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	1	23	theme	1D	375:376	arg1	NMR					385:387	1D and 2D NMR	375:387	1D and 2D NMR	375:387	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	23	theme	1D	375:376	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	24	theme	2D	382:383	arg1	NMR					385:387	1D and 2D NMR	375:387	1D and 2D NMR	375:387	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	24	theme	2D	382:383	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	10	25	from	resinae	1760:1766	arg1	PGM					1748:1750	a PGM	1746:1750	a PGM from C. resinae	1746:1766	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	8	26	theme	levels	1502:1507	arg1	secretion					1483:1491	a higher secretion	1474:1491	a higher secretion of TNF-α levels	1474:1507	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	0	27	theme	mechanisms	123:132	arg1	activation					98:107	the activation	94:107	the activation of macrophage mechanisms	94:132	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	3	28	theme	carbohydrate	859:870	arg1	moiety					872:877	the carbohydrate moiety	855:877	the carbohydrate moiety of the F. oxysporum PGM	855:901	The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated.					
29767770	2	29	link	-linked	460:466	arg1	residues					488:495	(1→6)-linked β-D-galactofuranose residues	455:495	(1→6)-linked β-D-galactofuranose residues	455:495	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	30	theme	galactomannan	395:407	arg1	component					409:417	The galactomannan component	391:417	The galactomannan component	391:417	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	0	31	theme	β-1,6-linked	0:11	arg1	peptidogalactomannan					35:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan	0:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum	0:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	1	32	theme	methods	304:310	arg1	combination					262:272	a combination	260:272	a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR)	260:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	7	33	dep	F.	1227:1228	arg1	oxysporum					1230:1238	F. oxysporum	1227:1238	F. oxysporum PGM	1227:1242	F. oxysporum PGM triggered TNF-α release by macrophages.					
29767770	3	34	theme	PGM	899:901	arg1	moiety					872:877	the carbohydrate moiety	855:877	the carbohydrate moiety of the F. oxysporum PGM	855:901	The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated.					
29767770	8	35	theme	TNF-α	1372:1376	arg1	levels					1387:1392	TNF-α cytokine levels	1372:1392	TNF-α cytokine levels	1372:1392	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	0	36	theme	rich	30:33	arg1	peptidogalactomannan					35:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan	0:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum	0:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	3	37	theme	F.	886:887	arg1	PGM					899:901	the F. oxysporum PGM	882:901	the F. oxysporum PGM	882:901	The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated.					
29767770	4	38	theme	antigenic	967:975	arg1	activity					977:984	the PGM antigenic activity	959:984	the PGM antigenic activity	959:984	Periodate oxidation abolished much of the PGM antigenic activity.					
29767770	8	39	theme	levels	1387:1392	arg1	increase					1360:1367	a significant increase	1346:1367	a significant increase of TNF-α cytokine levels	1346:1392	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	10	40	theme	serum	1648:1652	arg1	reativity					1654:1662	patient serum reativity	1640:1662	patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae	1640:1766	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	5	41	theme	strong	989:994	arg1	decrease					996:1003	A strong decrease	987:1003	A strong decrease in reactivity	987:1017	A strong decrease in reactivity was also observed with de-O-glycosylated PGM.					
29767770	2	42	theme	β-D-galactofuranose	468:486	arg1	residues					488:495	(1→6)-linked β-D-galactofuranose residues	455:495	(1→6)-linked β-D-galactofuranose residues	455:495	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	43	dep	chain	438:442	arg1	units					615:619	terminal nonreducing end units	590:619	terminal nonreducing end units	590:619	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	0	44	theme	oxysporum	68:76	arg1	peptidogalactomannan					35:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan	0:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum	0:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	1	45	theme	nuclear	347:353	arg1	NMR					385:387	1D and 2D NMR	375:387	1D and 2D NMR	375:387	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	45	theme	nuclear	347:353	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	2	46	theme	3-4	799:801	arg1	units					803:807	3-4 units	799:807	3-4 units that are (1→5)-interlinked	799:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	9	47	theme	IL-10	1598:1602	arg1	release					1613:1619	IL-10 cytokine release	1598:1619	IL-10 cytokine release	1598:1619	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	7	48	theme	TNF-α	1254:1258	arg1	release					1260:1266	TNF-α release	1254:1266	TNF-α release by macrophages	1254:1281	F. oxysporum PGM triggered TNF-α release by macrophages.					
29767770	10	49	theme	patient	1640:1646	arg1	reativity					1654:1662	patient serum reativity	1640:1662	patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae	1640:1766	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	8	50	from	PGM	1335:1337	arg1	removal					1293:1299	Chemical removal	1284:1299	Chemical removal of O-linked oligosaccharides from PGM	1284:1337	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	10	51	with	patients	1826:1833	arg1	aspergillosis					1840:1852	aspergillosis	1840:1852	aspergillosis	1840:1852	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	7	52	theme	F.	1227:1228	arg1	PGM					1240:1242	F. oxysporum PGM	1227:1242	F. oxysporum PGM	1227:1242	F. oxysporum PGM triggered TNF-α release by macrophages.					
29767770	2	53	theme	main	708:711	arg1	chain					713:717	a main chain	706:717	a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	706:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	10	54	from	patients	1826:1833	arg1	serum					1815:1819	serum	1815:1819	serum from patients with aspergillosis	1815:1852	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	10	55	from	F.	1681:1682	arg1	PGM					1672:1674	a PGM	1670:1674	a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae	1670:1766	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	2	56	theme	α-D-Manp	743:750	arg1	residues					752:759	(1→6)-linked α-D-Manp residues	730:759	(1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	730:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	57	contain	containing	719:728	arg1	chain					713:717	a main chain	706:717	a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	706:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	57	contain	containing	719:728	arg2	residues					752:759	(1→6)-linked α-D-Manp residues	730:759	(1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	730:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	8	58	link	O-linked	1304:1311	arg1	oligosaccharides					1313:1328	O-linked oligosaccharides	1304:1328	O-linked oligosaccharides	1304:1328	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	10	59	theme	potential	1877:1885	arg1	antigen					1898:1904	a potential diagnostic antigen	1875:1904	a potential diagnostic antigen	1875:1904	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	2	60	theme	nonreducing	599:609	arg1	units					615:619	terminal nonreducing end units	590:619	terminal nonreducing end units	590:619	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	6	61	theme	F.	1124:1125	arg1	phagocytosis					1137:1148	F. oxysporum phagocytosis	1124:1148	F. oxysporum phagocytosis	1124:1148	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	6	62	dep	F.	1124:1125	arg1	oxysporum					1127:1135	F. oxysporum	1124:1135	F. oxysporum phagocytosis	1124:1148	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	1	63	theme	Fusarium	207:214	arg1	oxysporum					216:224	Fusarium oxysporum	207:224	Fusarium oxysporum	207:224	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	2	64	contain	containing	514:523	arg2	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	64	contain	containing	514:523	arg2	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	64	contain	containing	514:523	arg1	chains					507:512	side chains	502:512	side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2)	502:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	0	65	from	activation	98:107	arg1	important					81:89	important	81:89	important	81:89	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	5	66	theme	de-O-glycosylated	1042:1058	arg1	PGM					1060:1062	de-O-glycosylated PGM	1042:1062	de-O-glycosylated PGM	1042:1062	A strong decrease in reactivity was also observed with de-O-glycosylated PGM.					
29767770	5	67	gly	de-O-glycosylated	1042:1058	arg1	PGM					1060:1062	de-O-glycosylated PGM	1042:1062	de-O-glycosylated PGM	1042:1062	A strong decrease in reactivity was also observed with de-O-glycosylated PGM.					
29767770	2	68	link	-linked	735:741	arg1	residues					752:759	(1→6)-linked α-D-Manp residues	730:759	(1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked	730:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	10	69	theme	more	1932:1935	arg1	sera					1937:1940	more sera	1932:1940	more sera	1932:1940	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	1	70	from	oxysporum	216:224	arg1	PGM					197:199	PGM	197:199	PGM	197:199	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	70	from	oxysporum	216:224	arg1	peptidogalactomannan					175:194	A peptidogalactomannan	173:194	A peptidogalactomannan (PGM) from Fusarium oxysporum	173:224	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	2	71	theme	-linked	553:559	arg1	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	71	theme	-linked	553:559	arg1	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	71	theme	-linked	553:559	arg1	1→2					572:574	1→2	572:574	1→2	572:574	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	8	72	theme	Chemical	1284:1291	arg1	removal					1293:1299	Chemical removal	1284:1299	Chemical removal of O-linked oligosaccharides from PGM	1284:1337	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	6	73	dep	F.	1205:1206	arg1	oxysporum					1208:1216	F. oxysporum	1205:1216	F. oxysporum	1205:1216	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	2	74	theme	side	502:505	arg1	chains					507:512	side chains	502:512	side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2)	502:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	8	75	theme	TNF-α	1496:1500	arg1	levels					1502:1507	TNF-α levels	1496:1507	TNF-α levels	1496:1507	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	6	76	gly	de-O-glycosylated	1078:1094	arg1	PGM					1096:1098	de-O-glycosylated PGM	1078:1098	de-O-glycosylated PGM	1078:1098	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	8	77	theme	oligosaccharides	1313:1328	arg1	removal					1293:1299	Chemical removal	1284:1299	Chemical removal of O-linked oligosaccharides from PGM	1284:1337	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	0	78	theme	macrophage	112:121	arg1	mechanisms					123:132	macrophage mechanisms	112:132	macrophage mechanisms	112:132	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	2	79	theme	-linked	530:536	arg1	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	79	theme	-linked	530:536	arg1	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	4	80	theme	Periodate	921:929	arg1	oxidation					931:939	Periodate oxidation	921:939	Periodate oxidation	921:939	Periodate oxidation abolished much of the PGM antigenic activity.					
29767770	0	81	theme	Galactofuranose-	13:28	arg1	peptidogalactomannan					35:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan	0:54	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum	0:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	2	82	dep	-β-D-Manp	561:569	arg1	1→2					549:551	1→2	549:551	1→2	549:551	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	9	83	theme	intact	1547:1552	arg1	PGM					1570:1572	intact and de-O-linked PGM	1547:1572	intact and de-O-linked PGM	1547:1572	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	3	84	theme	moiety	872:877	arg1	importance					841:850	The importance	837:850	The importance of the carbohydrate moiety of the F. oxysporum PGM	837:901	The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated.					
29767770	8	85	theme	significant	1348:1358	arg1	increase					1360:1367	a significant increase	1346:1367	a significant increase of TNF-α cytokine levels	1346:1392	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	9	86	theme	de-O-linked	1558:1568	arg1	PGM					1570:1572	intact and de-O-linked PGM	1547:1572	intact and de-O-linked PGM	1547:1572	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	2	87	theme	main	433:436	arg1	chain					438:442	a main chain	431:442	a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2)	431:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	0	88	theme	Fusarium	59:66	arg1	oxysporum					68:76	Fusarium oxysporum	59:76	Fusarium oxysporum	59:76	β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen.					
29767770	3	89	theme	oxysporum	889:897	arg1	PGM					899:901	the F. oxysporum PGM	882:901	the F. oxysporum PGM	882:901	The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated.					
29767770	4	90	theme	PGM	963:965	arg1	activity					977:984	the PGM antigenic activity	959:984	the PGM antigenic activity	959:984	Periodate oxidation abolished much of the PGM antigenic activity.					
29767770	8	91	theme	cytokine	1378:1385	arg1	levels					1387:1392	TNF-α cytokine levels	1372:1392	TNF-α cytokine levels	1372:1392	Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their removal could exposure another PGM motifs able to induce a higher secretion of TNF-α levels.					
29767770	2	92	theme	1→2	526:528	arg1	-β-D-Manp					561:569	(1→2)-linked -β-D-Manp	548:569	(1→2)-linked -β-D-Manp (1→2)	548:575	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	92	theme	1→2	526:528	arg1	α-D-Glcp					538:545	(1→2)-linked α-D-Glcp	525:545	(1→2)-linked α-D-Glcp	525:545	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	1	93	theme	two-dimensional	331:345	arg1	NMR					385:387	1D and 2D NMR	375:387	1D and 2D NMR	375:387	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	93	theme	two-dimensional	331:345	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	2	94	dep	residues	752:759	arg1	1→6					731:733	1→6	731:733	1→6	731:733	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	95	theme	-linked	460:466	arg1	residues					488:495	(1→6)-linked β-D-galactofuranose residues	455:495	(1→6)-linked β-D-galactofuranose residues	455:495	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	6	96	from	able	1108:1111	arg1	addition					1068:1075	addition	1068:1075	addition	1068:1075	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	1	97	theme	magnetic	355:362	arg1	NMR					385:387	1D and 2D NMR	375:387	1D and 2D NMR	375:387	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	1	97	theme	magnetic	355:362	arg1	resonance					364:372	two-dimensional nuclear magnetic resonance	331:372	two-dimensional nuclear magnetic resonance (1D and 2D NMR)	331:388	A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR).					
29767770	10	98	from	difference	1626:1635	arg1	reativity					1654:1662	patient serum reativity	1640:1662	patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae	1640:1766	The difference in patient serum reativity using a PGM from F. oxysporum characterized in the present study as compared with a PGM from C. resinae, that presents the same epitopes recognized by serum from patients with aspergillosis, could be considered a potential diagnostic antigen and should be tested with more sera.					
29767770	6	99	from	addition	1068:1075	arg1	able					1108:1111	able	1108:1111	able	1108:1111	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	9	100	link	de-O-linked	1558:1568	arg1	PGM					1570:1572	intact and de-O-linked PGM	1547:1572	intact and de-O-linked PGM	1547:1572	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	9	101	theme	cytokine	1604:1611	arg1	release					1613:1619	IL-10 cytokine release	1598:1619	IL-10 cytokine release	1598:1619	Interestingly, F. oxysporum conidia, intact and de-O-linked PGM were not able to induce IL-10 cytokine release.					
29767770	6	102	theme	de-O-glycosylated	1078:1094	arg1	PGM					1096:1098	de-O-glycosylated PGM	1078:1098	de-O-glycosylated PGM	1078:1098	In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM.					
29767770	2	103	theme	side	784:787	arg1	β-D-Galf					772:779	β-D-Galf	772:779	β-D-Galf	772:779	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
29767770	2	103	theme	side	784:787	arg1	chains					789:794	side chains	784:794	side chains of 3-4 units that are (1→5)-interlinked	784:834	The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked.					
30469516	11	0	theme	lines	1975:1979	arg1	growth					1946:1951	the growth	1942:1951	the growth of breast cancer cell lines	1942:1979	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	6	1	theme	cell	1182:1185	arg1	lines-MCF-7					1187:1197	two breast cancer cell lines-MCF-7	1164:1197	two breast cancer cell lines-MCF-7	1164:1197	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	8	2	theme	time-dependent	1524:1537	arg1	inhibition					1539:1548	A time-dependent inhibition	1522:1548	A time-dependent inhibition	1522:1548	A time-dependent inhibition was only observed in the MCF-7.					
30469516	6	3	theme	breast	1168:1173	arg1	lines-MCF-7					1187:1197	two breast cancer cell lines-MCF-7	1164:1197	two breast cancer cell lines-MCF-7	1164:1197	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	11	4	theme	unique	1897:1902	arg1	LMWF					1904:1907	a unique LMWF	1895:1907	a unique LMWF	1895:1907	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	4	5	theme	2	676:676	arg1	kDa					678:680	kDa	678:680	kDa	678:680	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	7	6	from	pinnatifida	1358:1368	arg1	weight					1252:1257	the molecular weight	1238:1257	the molecular weight	1238:1257	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	7	6	from	pinnatifida	1358:1368	arg1	factor					1273:1278	a critical factor	1262:1278	a critical factor	1262:1278	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	2	7	theme	laboratory-grade	562:577	arg1	fucoidan					579:586	the laboratory-grade fucoidan	558:586	the laboratory-grade fucoidan from Sigma (S)	558:601	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	7	8	theme	anti-cancer	1299:1309	arg1	potential					1311:1319	the anti-cancer potential	1295:1319	the anti-cancer potential of fucoidan	1295:1331	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	0	9	theme	Molecular	128:136	arg1	Fraction					145:152	Unique Low Molecular Weight Fraction	117:152	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	1	10	theme	complex	209:215	arg1	Fucoidan					195:202	Fucoidan	195:202	Fucoidan	195:202	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	1	10	theme	complex	209:215	arg1	polysaccharide					245:258	the complex fucose-containing sulphated polysaccharide	205:258	the complex fucose-containing sulphated polysaccharide	205:258	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	9	11	theme	caspase-dependent	1595:1611	arg1	apoptosis					1613:1621	caspase-dependent apoptosis	1595:1621	caspase-dependent apoptosis	1595:1621	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	0	12	theme	Fraction	145:152	arg1	Bioactivity					154:164	Unique Low Molecular Weight Fraction Bioactivity	117:164	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	1	13	theme	sulphated	235:243	arg1	Fucoidan					195:202	Fucoidan	195:202	Fucoidan	195:202	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	1	13	theme	sulphated	235:243	arg1	polysaccharide					245:258	the complex fucose-containing sulphated polysaccharide	205:258	the complex fucose-containing sulphated polysaccharide	205:258	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	7	14	theme	cell	1510:1513	arg1	lines					1515:1519	both cell lines	1505:1519	both cell lines	1505:1519	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	4	15	theme	other	831:835	arg1	fucoidans					842:850	the other four fucoidans	827:850	the other four fucoidans	827:850	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	6	16	theme	weight	1138:1143	arg1	HMWF					1155:1158	HMWF	1155:1158	HMWF	1155:1158	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	6	16	theme	weight	1138:1143	arg1	fucoidan					1145:1152	a high molecular weight fucoidan	1121:1152	a high molecular weight fucoidan (HMWF)	1121:1159	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	11	17	theme	cancer	1963:1968	arg1	lines					1975:1979	breast cancer cell lines	1956:1979	breast cancer cell lines	1956:1979	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	6	18	theme	high	1123:1126	arg1	weight					1138:1143	a high molecular weight	1121:1143	a high molecular weight fucoidan (HMWF)	1121:1159	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	0	19	theme	Cancer	176:181	arg1	Lines					188:192	Breast Cancer Cell Lines	169:192	Breast Cancer Cell Lines	169:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	5	20	theme	cancer	1001:1006	arg1	lines					1013:1017	some cancer cell lines	996:1017	some cancer cell lines	996:1017	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	9	21	theme	MDA-MB-231	1643:1652	arg1	cells					1654:1658	the MDA-MB-231 cells	1639:1658	the MDA-MB-231 cells	1639:1658	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	4	22	theme	low	682:684	arg1	weight					696:701	2 kDa low molecular weight	676:701	2 kDa low molecular weight fraction (LMWF)	676:717	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	7	23	theme	cells	1459:1463	arg1	proliferation					1428:1440	the proliferation	1424:1440	the proliferation of breast cancer cells	1424:1463	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	9	24	theme	apoptosis	1683:1691	arg1	pathway					1693:1699	the intrinsic apoptosis pathway	1669:1699	the intrinsic apoptosis pathway alone	1669:1705	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	0	25	theme	Unique	117:122	arg1	Fraction					145:152	Unique Low Molecular Weight Fraction	117:152	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	0	26	dep	Fucoidans	106:114	arg1	Bioactivity					154:164	Unique Low Molecular Weight Fraction Bioactivity	117:164	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	7	27	theme	molecular	1242:1250	arg1	weight					1252:1257	the molecular weight	1238:1257	the molecular weight	1238:1257	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	7	27	theme	molecular	1242:1250	arg1	factor					1273:1278	a critical factor	1262:1278	a critical factor	1262:1278	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	2	28	theme	same	455:458	arg1	pinnatifida					484:494	the same seaweed species Undaria pinnatifida	451:494	the same seaweed species Undaria pinnatifida	451:494	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	2	29	theme	geological	515:524	arg1	locations					526:534	different geological locations	505:534	different geological locations	505:534	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	5	30	theme	compositions	881:892	arg1	difference					857:866	The difference	853:866	The difference of molecular compositions between S and S1	853:909	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	2	31	from	S	600:600	arg1	fucoidan					579:586	the laboratory-grade fucoidan	558:586	the laboratory-grade fucoidan from Sigma (S)	558:601	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	4	32	theme	Zealand	730:736	arg1	larger					762:767	larger	762:767	larger	762:767	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	32	theme	Zealand	730:736	arg1	Zealand					730:736	the New Zealand crude fucoidan (S1)	722:756	the New Zealand crude fucoidan (S1)	722:756	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	32	theme	Zealand	730:736	arg1	amount					661:666	The amount	657:666	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1)	657:756	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	0	33	dep	Comparison	76:85	arg1	Undaria					40:46	Undaria	40:46	Undaria	40:46	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	0	34	theme	New	28:30	arg1	Zealand					32:38	the New Zealand	24:38	the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	24:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	2	35	theme	Undaria	476:482	arg1	pinnatifida					484:494	the same seaweed species Undaria pinnatifida	451:494	the same seaweed species Undaria pinnatifida	451:494	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	5	36	theme	different	964:972	arg1	profile					985:991	different anticancer profile	964:991	different anticancer profile	964:991	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	2	37	theme	species	468:474	arg1	pinnatifida					484:494	the same seaweed species Undaria pinnatifida	451:494	the same seaweed species Undaria pinnatifida	451:494	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	12	38	theme	cancer	2064:2069	arg1	treatment					2071:2079	a supplement cancer treatment	2051:2079	a supplement cancer treatment	2051:2079	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	12	38	theme	cancer	2064:2069	arg1	LMWF					1997:2000	the LMWF	1993:2000	the LMWF from New Zealand U. pinnatifida	1993:2032	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	4	39	dep	unique	807:812	arg1	compared					815:822	compared	815:822	compared to the other four fucoidans	815:850	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	6	40	theme	unique	1060:1065	arg1	LMWF					1067:1070	this unique LMWF	1055:1070	this unique LMWF	1055:1070	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	10	41	theme	NOS	1772:1774	arg1	activation					1776:1785	a dose-dependent NOS activation	1755:1785	a dose-dependent NOS activation	1755:1785	LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells.					
30469516	12	42	used	used	2043:2046	arg2	treatment					2071:2079	a supplement cancer treatment	2051:2079	a supplement cancer treatment	2051:2079	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	12	42	used	used	2043:2046	arg2	LMWF					1997:2000	the LMWF	1993:2000	the LMWF from New Zealand U. pinnatifida	1993:2032	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	10	43	theme	MDA-MB-231	1794:1803	arg1	cells					1805:1809	the MDA-MB-231 cells	1790:1809	the MDA-MB-231 cells	1790:1809	LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells.					
30469516	6	44	theme	cancer	1175:1180	arg1	lines-MCF-7					1187:1197	two breast cancer cell lines-MCF-7	1164:1197	two breast cancer cell lines-MCF-7	1164:1197	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	12	45	from	pinnatifida	2022:2032	arg1	treatment					2071:2079	a supplement cancer treatment	2051:2079	a supplement cancer treatment	2051:2079	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	12	45	from	pinnatifida	2022:2032	arg1	LMWF					1997:2000	the LMWF	1993:2000	the LMWF from New Zealand U. pinnatifida	1993:2032	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	6	46	theme	cytotoxic	1089:1097	arg1	effects					1099:1105	the cytotoxic effects	1085:1105	the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231	1085:1212	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	7	47	theme	fucoidan	1324:1331	arg1	potential					1311:1319	the anti-cancer potential	1295:1319	the anti-cancer potential of fucoidan	1295:1331	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	0	48	theme	Weight	138:143	arg1	Fraction					145:152	Unique Low Molecular Weight Fraction	117:152	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	11	49	theme	breast	1956:1961	arg1	lines					1975:1979	breast cancer cell lines	1956:1979	breast cancer cell lines	1956:1979	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	1	50	theme	fucose-containing	217:233	arg1	Fucoidan					195:202	Fucoidan	195:202	Fucoidan	195:202	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	1	50	theme	fucose-containing	217:233	arg1	polysaccharide					245:258	the complex fucose-containing sulphated polysaccharide	205:258	the complex fucose-containing sulphated polysaccharide	205:258	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	9	51	theme	apoptosis	1613:1621	arg1	Induction					1582:1590	Induction	1582:1590	Induction of caspase-dependent apoptosis	1582:1621	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	11	52	contain	contains	1886:1893	arg2	fucoidan					1831:1838	the fucoidan	1827:1838	the fucoidan extracted from the New Zealand U. pinnatifida	1827:1884	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	11	52	contain	contains	1886:1893	arg2	LMWF					1904:1907	a unique LMWF	1895:1907	a unique LMWF	1895:1907	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	11	52	contain	contains	1886:1893	arg1	conclusion					1815:1824	conclusion	1815:1824	conclusion	1815:1824	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	11	52	contain	contains	1886:1893	arg1	fucoidan					1831:1838	the fucoidan	1827:1838	the fucoidan extracted from the New Zealand U. pinnatifida	1827:1884	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	5	53	dep	observation	934:944	arg1	exhibited					954:962	exhibited	954:962	exhibited	954:962	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	0	54	theme	Breast	169:174	arg1	Lines					188:192	Breast Cancer Cell Lines	169:192	Breast Cancer Cell Lines	169:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	4	55	dep	Zealand	730:736	arg1	fucoidan					744:751	crude fucoidan	738:751	the New Zealand crude fucoidan (S1)	722:756	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	55	dep	Zealand	730:736	arg1	S1					754:755	S1	754:755	S1	754:755	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	2	56	theme	Sigma	593:597	arg1	S					600:600	Sigma (S)	593:601	Sigma (S)	593:601	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	6	57	theme	molecular	1128:1136	arg1	weight					1138:1143	a high molecular weight	1121:1143	a high molecular weight fucoidan (HMWF)	1121:1159	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	0	58	theme	Cell	183:186	arg1	Lines					188:192	Breast Cancer Cell Lines	169:192	Breast Cancer Cell Lines	169:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	11	59	theme	cell	1970:1973	arg1	lines					1975:1979	breast cancer cell lines	1956:1979	breast cancer cell lines	1956:1979	In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines.					
30469516	7	60	theme	critical	1264:1271	arg1	weight					1252:1257	the molecular weight	1238:1257	the molecular weight	1238:1257	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	7	60	theme	critical	1264:1271	arg1	factor					1273:1278	a critical factor	1262:1278	a critical factor	1262:1278	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	5	61	theme	cell	1008:1011	arg1	lines					1013:1017	some cancer cell lines	996:1017	some cancer cell lines	996:1017	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	9	62	located	observed	1627:1634	arg2	Induction					1582:1590	Induction	1582:1590	Induction of caspase-dependent apoptosis	1582:1621	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	9	62	located	observed	1627:1634	arg1	cells					1654:1658	the MDA-MB-231 cells	1639:1658	the MDA-MB-231 cells	1639:1658	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	2	63	theme	different	505:513	arg1	locations					526:534	different geological locations	505:534	different geological locations	505:534	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	6	64	theme	LMWF	1112:1115	arg1	effects					1099:1105	the cytotoxic effects	1085:1105	the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231	1085:1212	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	4	65	theme	kDa	678:680	arg1	weight					696:701	2 kDa low molecular weight	676:701	2 kDa low molecular weight fraction (LMWF)	676:717	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	0	66	theme	Other	100:104	arg1	Fucoidans					106:114	Five Other Fucoidans	95:114	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	4	67	theme	molecular	686:694	arg1	weight					696:701	2 kDa low molecular weight	676:701	2 kDa low molecular weight fraction (LMWF)	676:717	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	68	dep	weight	696:701	arg1	LMWF					713:716	LMWF	713:716	LMWF	713:716	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	68	dep	weight	696:701	arg1	fraction					703:710	fraction	703:710	2 kDa low molecular weight fraction (LMWF)	676:717	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	7	69	theme	cancer	1452:1457	arg1	cells					1459:1463	breast cancer cells	1445:1463	breast cancer cells	1445:1463	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	9	70	theme	intrinsic	1673:1681	arg1	pathway					1693:1699	the intrinsic apoptosis pathway	1669:1699	the intrinsic apoptosis pathway alone	1669:1705	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	4	71	theme	crude	738:742	arg1	fucoidan					744:751	crude fucoidan	738:751	the New Zealand crude fucoidan (S1)	722:756	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	4	71	theme	crude	738:742	arg1	S1					754:755	S1	754:755	S1	754:755	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	6	72	from	effects	1099:1105	arg1	MDA-MB-231					1203:1212	MDA-MB-231	1203:1212	MDA-MB-231	1203:1212	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	6	72	from	effects	1099:1105	arg1	lines-MCF-7					1187:1197	two breast cancer cell lines-MCF-7	1164:1197	two breast cancer cell lines-MCF-7	1164:1197	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	0	73	theme	Low	124:126	arg1	Fraction					145:152	Unique Low Molecular Weight Fraction	117:152	Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	95:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	1	74	theme	extraction	375:384	arg1	method					386:391	extraction method	375:391	extraction method	375:391	Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method.					
30469516	5	75	theme	molecular	871:879	arg1	compositions					881:892	molecular compositions	871:892	molecular compositions	871:892	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	10	76	theme	dose-dependent	1757:1770	arg1	activation					1776:1785	a dose-dependent NOS activation	1755:1785	a dose-dependent NOS activation	1755:1785	LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells.					
30469516	4	77	theme	New	726:728	arg1	Zealand					730:736	the New Zealand crude fucoidan (S1)	722:756	the New Zealand crude fucoidan (S1)	722:756	The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans.					
30469516	7	78	theme	breast	1445:1450	arg1	cells					1459:1463	breast cancer cells	1445:1463	breast cancer cells	1445:1463	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30469516	9	79	theme	extrinsic	1720:1728	arg1	pathway					1730:1736	the extrinsic pathway	1716:1736	the extrinsic pathway	1716:1736	Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway.					
30469516	3	80	theme	molecular	634:642	arg1	composition					644:654	molecular composition	634:654	molecular composition	634:654	The five products differed in molecular composition.					
30469516	0	81	theme	pinnatifida-Physicochemical	48:74	arg1	Comparison					76:85	pinnatifida-Physicochemical Comparison	48:85	pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines	48:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	8	82	located	observed	1559:1566	arg2	inhibition					1539:1548	A time-dependent inhibition	1522:1548	A time-dependent inhibition	1522:1548	A time-dependent inhibition was only observed in the MCF-7.					
30469516	8	82	located	observed	1559:1566	arg1	MCF-7					1575:1579	the MCF-7	1571:1579	the MCF-7	1571:1579	A time-dependent inhibition was only observed in the MCF-7.					
30469516	5	83	theme	anticancer	974:983	arg1	profile					985:991	different anticancer profile	964:991	different anticancer profile	964:991	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	2	84	theme	seaweed	460:466	arg1	pinnatifida					484:494	the same seaweed species Undaria pinnatifida	451:494	the same seaweed species Undaria pinnatifida	451:494	In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S).					
30469516	12	85	theme	supplement	2053:2062	arg1	treatment					2071:2079	a supplement cancer treatment	2051:2079	a supplement cancer treatment	2051:2079	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	12	85	theme	supplement	2053:2062	arg1	LMWF					1997:2000	the LMWF	1993:2000	the LMWF from New Zealand U. pinnatifida	1993:2032	Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.					
30469516	5	86	theme	previous	925:932	arg1	observation					934:944	our previous observation	921:944	our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S	921:1034	The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S.					
30469516	6	87	theme	fucoidan	1145:1152	arg1	effects					1099:1105	the cytotoxic effects	1085:1105	the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231	1085:1212	Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines-MCF-7 and MDA-MB-231.					
30469516	0	88	from	Bioactivity	154:164	arg1	Lines					188:192	Breast Cancer Cell Lines	169:192	Breast Cancer Cell Lines	169:192	Fucoidan Extracted from the New Zealand Undaria pinnatifida-Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines.					
30469516	7	89	theme	dose-dependent	1395:1408	arg1	inhibition					1410:1419	a dose-dependent inhibition	1393:1419	a dose-dependent inhibition	1393:1419	Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines.					
30506250	3	0	theme	identification	714:727	arg1	methods					729:735	identification methods	714:735	identification methods	714:735	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	0	1	from	Analysis	11:18	arg1	Bacteria					59:66	Lactic Acid Bacteria	47:66	Lactic Acid Bacteria	47:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	4	2	theme	different	863:871	arg1	exopolysaccharides					873:890	two different exopolysaccharides	859:890	two different exopolysaccharides from lactic acid bacteria	859:916	In this chapter, some analytical methods are described and demonstrated for two different exopolysaccharides from lactic acid bacteria.					
30506250	2	3	theme	substituents	370:381	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	3	theme	substituents	370:381	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	3	theme	substituents	370:381	arg1	presence					322:329	the presence	318:329	the presence of repeating units and noncarbohydrate substituents	318:381	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	3	theme	substituents	370:381	arg1	composition					243:253	the monosaccharide composition	224:253	the monosaccharide composition	224:253	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	3	theme	substituents	370:381	arg1	presentation					400:411	the presentation	396:411	the presentation of a chemical molecular structure or composite model	396:464	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	4	dep	SEC-chromatography	674:691	arg1	e.g.					668:671	e.g.	668:671	e.g.	668:671	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	5	dep	such	591:594	arg1	as					596:597	as	596:597	as	596:597	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	4	6	theme	lactic	897:902	arg1	bacteria					909:916	lactic acid bacteria	897:916	lactic acid bacteria	897:916	In this chapter, some analytical methods are described and demonstrated for two different exopolysaccharides from lactic acid bacteria.					
30506250	2	7	theme	linkages	308:315	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	7	theme	linkages	308:315	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	0	8	from	Bacteria	59:66	arg1	Exopolysaccharides					23:40	Exopolysaccharides	23:40	Exopolysaccharides from Lactic Acid Bacteria	23:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	0	8	from	Bacteria	59:66	arg1	Analysis					11:18	Structural Analysis	0:18	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria	0:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	3	9	theme	techniques	579:588	arg1	use					562:564	the use	558:564	the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy)	558:780	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	10	theme	glycosidic	297:306	arg1	linkages					308:315	glycosidic linkages	297:315	glycosidic linkages	297:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	11	dep	hydrolysis	635:644	arg1	e.g.					629:632	e.g.	629:632	e.g.	629:632	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	12	theme	Structural	150:159	arg1	information					161:171	Structural information	150:171	Structural information of these widely diverse biopolymers	150:207	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	1	13	theme	exopolysaccharides	83:100	arg1	phenomenon					138:147	a common phenomenon	129:147	a common phenomenon	129:147	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	1	13	theme	exopolysaccharides	83:100	arg1	Production					69:78	Production	69:78	Production of exopolysaccharides by lactic acid bacteria	69:124	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	4	14	theme	acid	904:907	arg1	bacteria					909:916	lactic acid bacteria	897:916	lactic acid bacteria	897:916	In this chapter, some analytical methods are described and demonstrated for two different exopolysaccharides from lactic acid bacteria.					
30506250	0	15	theme	Structural	0:9	arg1	Analysis					11:18	Structural Analysis	0:18	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria	0:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	3	16	theme	1H/13C	757:762	arg1	spectroscopy					768:779	1H/13C NMR spectroscopy	757:779	1H/13C NMR spectroscopy	757:779	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	1	17	theme	lactic	105:110	arg1	bacteria					117:124	lactic acid bacteria	105:124	lactic acid bacteria	105:124	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	0	18	theme	Exopolysaccharides	23:40	arg1	Analysis					11:18	Structural Analysis	0:18	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria	0:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	3	19	theme	NMR	764:766	arg1	spectroscopy					768:779	1H/13C NMR spectroscopy	757:779	1H/13C NMR spectroscopy	757:779	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	20	theme	methods	620:626	arg1	use					562:564	the use	558:564	the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy)	558:780	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	1	21	theme	acid	112:115	arg1	bacteria					117:124	lactic acid bacteria	105:124	lactic acid bacteria	105:124	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	2	22	theme	composite	450:458	arg1	model					460:464	composite model	450:464	composite model	450:464	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	23	theme	biopolymers	197:207	arg1	information					161:171	Structural information	150:171	Structural information of these widely diverse biopolymers	150:207	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	24	theme	structure	437:445	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	24	theme	structure	437:445	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	24	theme	structure	437:445	arg1	presence					322:329	the presence	318:329	the presence of repeating units and noncarbohydrate substituents	318:381	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	24	theme	structure	437:445	arg1	composition					243:253	the monosaccharide composition	224:253	the monosaccharide composition	224:253	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	24	theme	structure	437:445	arg1	presentation					400:411	the presentation	396:411	the presentation of a chemical molecular structure or composite model	396:464	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	0	25	theme	Lactic	47:52	arg1	Bacteria					59:66	Lactic Acid Bacteria	47:66	Lactic Acid Bacteria	47:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	2	26	theme	diverse	189:195	arg1	biopolymers					197:207	these widely diverse biopolymers	176:207	these widely diverse biopolymers	176:207	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	27	theme	molecular	427:435	arg1	structure					437:445	a chemical molecular structure	416:445	a chemical molecular structure	416:445	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	28	theme	separation	648:657	arg1	methods					659:665	separation methods	648:665	separation methods (e.g., SEC-chromatography and HPLC/HPAEC)	648:707	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	29	theme	model	460:464	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	29	theme	model	460:464	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	29	theme	model	460:464	arg1	presence					322:329	the presence	318:329	the presence of repeating units and noncarbohydrate substituents	318:381	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	29	theme	model	460:464	arg1	composition					243:253	the monosaccharide composition	224:253	the monosaccharide composition	224:253	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	29	theme	model	460:464	arg1	presentation					400:411	the presentation	396:411	the presentation of a chemical molecular structure or composite model	396:464	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	30	theme	chemical	418:425	arg1	structure					437:445	a chemical molecular structure	416:445	a chemical molecular structure	416:445	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	31	theme	methods	659:665	arg1	use					562:564	the use	558:564	the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy)	558:780	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	32	theme	degradation	608:618	arg1	methods					620:626	chemical degradation methods	599:626	chemical degradation methods (e.g., hydrolysis)	599:645	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	33	theme	methods	729:735	arg1	use					562:564	the use	558:564	the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy)	558:780	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	34	theme	noncarbohydrate	354:368	arg1	substituents					370:381	noncarbohydrate substituents	354:381	noncarbohydrate substituents	354:381	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	0	35	theme	Acid	54:57	arg1	Bacteria					59:66	Lactic Acid Bacteria	47:66	Lactic Acid Bacteria	47:66	Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria.					
30506250	3	36	theme	polysaccharides	503:517	arg1	analysis					491:498	The detailed structural analysis	467:498	The detailed structural analysis of polysaccharides	467:517	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	36	theme	polysaccharides	503:517	arg1	pursuit					539:545	a time-consuming pursuit	522:545	a time-consuming pursuit	522:545	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	37	theme	monosaccharide	228:241	arg1	composition					243:253	the monosaccharide composition	224:253	the monosaccharide composition	224:253	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	38	theme	time-consuming	524:537	arg1	analysis					491:498	The detailed structural analysis	467:498	The detailed structural analysis of polysaccharides	467:517	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	38	theme	time-consuming	524:537	arg1	pursuit					539:545	a time-consuming pursuit	522:545	a time-consuming pursuit	522:545	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	4	39	theme	analytical	805:814	arg1	methods					816:822	some analytical methods	800:822	some analytical methods	800:822	In this chapter, some analytical methods are described and demonstrated for two different exopolysaccharides from lactic acid bacteria.					
30506250	1	40	theme	common	131:136	arg1	phenomenon					138:147	a common phenomenon	129:147	a common phenomenon	129:147	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	1	40	theme	common	131:136	arg1	Production					69:78	Production	69:78	Production of exopolysaccharides by lactic acid bacteria	69:124	Production of exopolysaccharides by lactic acid bacteria is a common phenomenon.					
30506250	4	41	from	bacteria	909:916	arg1	exopolysaccharides					873:890	two different exopolysaccharides	859:890	two different exopolysaccharides from lactic acid bacteria	859:916	In this chapter, some analytical methods are described and demonstrated for two different exopolysaccharides from lactic acid bacteria.					
30506250	3	42	theme	different	569:577	arg1	techniques					579:588	different techniques	569:588	different techniques	569:588	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	43	theme	repeating	334:342	arg1	units					344:348	repeating units	334:348	repeating units	334:348	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	44	theme	units	344:348	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	44	theme	units	344:348	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	44	theme	units	344:348	arg1	presence					322:329	the presence	318:329	the presence of repeating units and noncarbohydrate substituents	318:381	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	44	theme	units	344:348	arg1	composition					243:253	the monosaccharide composition	224:253	the monosaccharide composition	224:253	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	44	theme	units	344:348	arg1	presentation					400:411	the presentation	396:411	the presentation of a chemical molecular structure or composite model	396:464	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	45	theme	structural	480:489	arg1	analysis					491:498	The detailed structural analysis	467:498	The detailed structural analysis of polysaccharides	467:517	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	45	theme	structural	480:489	arg1	pursuit					539:545	a time-consuming pursuit	522:545	a time-consuming pursuit	522:545	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	46	theme	detailed	471:478	arg1	analysis					491:498	The detailed structural analysis	467:498	The detailed structural analysis of polysaccharides	467:517	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	46	theme	detailed	471:478	arg1	pursuit					539:545	a time-consuming pursuit	522:545	a time-consuming pursuit	522:545	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	3	47	dep	GLC-EIMS	744:751	arg1	e.g.					738:741	e.g.	738:741	e.g.	738:741	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
30506250	2	48	theme	anomeric	260:267	arg1	configurations					269:282	the anomeric configurations	256:282	the anomeric configurations	256:282	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	2	48	theme	anomeric	260:267	arg1	type					289:292	the type	285:292	the type of glycosidic linkages	285:315	Structural information of these widely diverse biopolymers is rendered by the monosaccharide composition, the anomeric configurations, the type of glycosidic linkages, the presence of repeating units and noncarbohydrate substituents, and finally the presentation of a chemical molecular structure or composite model.					
30506250	3	49	theme	chemical	599:606	arg1	methods					620:626	chemical degradation methods	599:626	chemical degradation methods (e.g., hydrolysis)	599:645	The detailed structural analysis of polysaccharides is a time-consuming pursuit, including the use of different techniques, such as chemical degradation methods (e.g., hydrolysis), separation methods (e.g., SEC-chromatography and HPLC/HPAEC), and identification methods (e.g., GLC-EIMS and 1H/13C NMR spectroscopy).					
31137802	1	0	attach	isolated	134:141	arg2	kind					179:182	a kind	177:182	a kind of macromolecule with some biological activities	177:231	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	1	0	attach	isolated	134:141	arg2	polysaccharide					119:132	The polysaccharide	115:132	The polysaccharide isolated from alfalfa	115:154	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	1	0	attach	isolated	134:141	arg1	alfalfa					148:154	alfalfa	148:154	alfalfa	148:154	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	6	1	theme	number-average	1173:1186	arg1	Mn					1206:1207	Mn	1206:1207	Mn	1206:1207	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	1	theme	number-average	1173:1186	arg1	weight					1198:1203	number-average molecular weight	1173:1203	number-average molecular weight (Mn)	1173:1208	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	2	theme	chromatography-refractive	1380:1404	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	2	theme	chromatography-refractive	1380:1404	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	3	3	theme	membrane	684:691	arg1	dialysis					693:700	semipermeable membrane dialysis	670:700	semipermeable membrane dialysis	670:700	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	1	4	from	effects	271:277	arg1	cells					289:293	immune cells	282:293	immune cells	282:293	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	6	5	theme	gel	1365:1367	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	5	theme	gel	1365:1367	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	1	6	from	structure	257:265	arg1	cells					289:293	immune cells	282:293	immune cells	282:293	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	4	7	dep	farms	759:763	arg1	sample					780:785	one composite sample	766:785	one composite sample per farm	766:794	Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination.					
31137802	2	8	theme	extraction	363:372	arg1	methods					388:394	the extraction and purifying methods	359:394	methods	388:394	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	7	9	theme	%	1741:1741	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	10	theme	mass	1497:1500	arg1	EI-MS					1516:1520	EI-MS	1516:1520	EI-MS	1516:1520	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	10	theme	mass	1497:1500	arg1	spectrometry					1502:1513	electron ionization mass spectrometry	1477:1513	electron ionization mass spectrometry (EI-MS)	1477:1521	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	6	11	theme	Z-average	1215:1223	arg1	weight					1235:1240	Z-average molecular weight	1215:1240	Z-average molecular weight (Mz) of APS	1215:1252	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	0	12	theme	Molecular	17:25	arg1	Characteristics					27:41	Molecular Characteristics	17:41	Molecular Characteristics	17:41	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	1	13	theme	macromolecule	187:199	arg1	kind					179:182	a kind	177:182	a kind of macromolecule with some biological activities	177:231	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	1	13	theme	macromolecule	187:199	arg1	polysaccharide					119:132	The polysaccharide	115:132	The polysaccharide isolated from alfalfa	115:154	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	7	14	theme	electron	1477:1484	arg1	EI-MS					1516:1520	EI-MS	1516:1520	EI-MS	1516:1520	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	14	theme	electron	1477:1484	arg1	spectrometry					1502:1513	electron ionization mass spectrometry	1477:1513	electron ionization mass spectrometry (EI-MS)	1477:1521	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	6	15	theme	weight-average	1135:1148	arg1	×					1281:1281	3.30 × 106	1276:1285	3.30 × 106	1276:1285	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	15	theme	weight-average	1135:1148	arg1	Mw					1168:1169	Mw	1168:1169	Mw	1168:1169	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	15	theme	weight-average	1135:1148	arg1	weight					1160:1165	The weight-average molecular weight	1131:1165	The weight-average molecular weight (Mw)	1131:1170	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	8	16	theme	T	1964:1964	arg1	cells					1966:1970	T cells	1964:1970	T cells	1964:1970	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	5	17	theme	chromatography	888:901	arg1	analysis					908:915	ion chromatography (IC) analysis	884:915	ion chromatography (IC) analysis	884:915	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	6	18	theme	laser	1423:1427	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	18	theme	laser	1423:1427	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	7	19	theme	%	1733:1733	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	1,4-Glc					1645:1651	1,4-Glc	1645:1651	1,4-Glc	1645:1651	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	galactose					1593:1601	galactose	1593:1601	galactose (T-D-Glc)	1593:1611	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	residues					1566:1573	seven linkage residues	1552:1573	seven linkage residues	1552:1573	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	1,3,4-GalA					1667:1676	1,3,4-GalA	1667:1676	1,3,4-GalA	1667:1676	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	1,4-Gal-Ac					1633:1642	1,4-Gal-Ac	1633:1642	1,4-Gal-Ac	1633:1642	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	glucose					1614:1620	glucose	1614:1620	glucose (T-D-Gal)	1614:1630	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	20	theme	linkage	1558:1564	arg1	1,5-Araf					1583:1590	1,5-Araf	1583:1590	1,5-Araf	1583:1590	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	9	21	theme	pharmaceutical	2167:2180	arg1	agent					2182:2186	an immunopotentiating pharmaceutical agent	2145:2186	an immunopotentiating pharmaceutical agent	2145:2186	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	8	22	from	expression	1950:1959	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	23	dep	cytokine	1919:1926	arg1	-4					1935:1936	-4	1935:1936	-4	1935:1936	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	23	dep	cytokine	1919:1926	arg1	IFN-γ					1943:1947	IFN-γ	1943:1947	IFN-γ	1943:1947	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	23	dep	cytokine	1919:1926	arg1	IL-2					1929:1932	IL-2	1929:1932	IL-2	1929:1932	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	0	24	theme	sativa	103:108	arg1	L					110:110	Medicago sativa L	94:110	Medicago sativa L.	94:111	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	25	from	Characteristics	27:41	arg1	Alfalfa					85:91	Alfalfa	85:91	Alfalfa	85:91	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	26	theme	Extract	0:6	arg1	Methods					8:14	Extract Methods	0:14	Extract Methods	0:14	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	1	27	theme	molecular	247:255	arg1	structure					257:265	its molecular structure	243:265	its molecular structure	243:265	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	7	28	theme	%	1717:1717	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	8	29	theme	B-cell	1813:1818	arg1	proliferation					1820:1832	B-cell proliferation	1813:1832	B-cell proliferation	1813:1832	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	2	30	theme	alfalfa	399:405	arg1	APS					444:446	APS	444:446	APS	444:446	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	2	30	theme	alfalfa	399:405	arg1	polysaccharide					428:441	alfalfa (Medicago sativa L.) polysaccharide	399:441	alfalfa (Medicago sativa L.) polysaccharide (APS)	399:447	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	9	31	theme	potential	2122:2130	arg1	candidate					2132:2140	a potential candidate	2120:2140	a potential candidate as an immunopotentiating pharmaceutical agent or functional food	2120:2205	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	4	32	theme	samples	727:733	arg1	batches					708:714	Five batches	703:714	Five batches of alfalfa samples	703:733	Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination.					
31137802	9	33	theme	present	1993:1999	arg1	results					2001:2007	the present results	1989:2007	the present results	1989:2007	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	2	34	theme	polysaccharide	428:441	arg1	methods					388:394	the extraction and purifying methods	359:394	methods	388:394	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	5	35	theme	glucuronic	1050:1059	arg1	acid					1061:1064	glucuronic acid	1050:1064	glucuronic acid	1050:1064	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	1	36	with	kind	179:182	arg1	activities					222:231	some biological activities	206:231	some biological activities	206:231	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	8	37	from	proliferation	1820:1832	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	7	38	theme	1,6-Gal	1654:1660	arg1	residues					1566:1573	seven linkage residues	1552:1573	seven linkage residues	1552:1573	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	38	theme	1,6-Gal	1654:1660	arg1	1,5-Araf					1583:1590	1,5-Araf	1583:1590	1,5-Araf	1583:1590	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	9	39	theme	macromolecular	2030:2043	arg1	APS					2022:2024	APS	2022:2024	APS	2022:2024	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	9	39	theme	macromolecular	2030:2043	arg1	polymers					2045:2052	macromolecular polymers	2030:2052	macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol	2030:2107	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	5	40	from	analysis	908:915	arg1	results					871:877	The results	867:877	The results from ion chromatography (IC) analysis	867:915	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	3	41	theme	alcohol	623:629	arg1	methods					645:651	alcohol precipitation methods	623:651	alcohol precipitation methods	623:651	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	4	42	theme	composite	770:778	arg1	sample					780:785	one composite sample	766:785	one composite sample per farm	766:794	Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination.					
31137802	2	43	theme	study	337:341	arg1	objectives					318:327	The objectives	314:327	The objectives of this study	314:341	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	1	44	theme	biological	211:220	arg1	activities					222:231	some biological activities	206:231	some biological activities	206:231	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	3	45	theme	semipermeable	670:682	arg1	dialysis					693:700	semipermeable membrane dialysis	670:700	semipermeable membrane dialysis	670:700	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	9	46	theme	molar	2061:2065	arg1	mass					2067:2070	a molar mass	2059:2070	a molar mass (indicated by Mw) of 3.3 × 106 g/mol	2059:2107	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	6	47	theme	×	1309:1309	arg1	g/mol					1315:1319	1.43 × 108 g/mol	1304:1319	1.43 × 108 g/mol	1304:1319	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	48	theme	molecular	1188:1196	arg1	Mn					1206:1207	Mn	1206:1207	Mn	1206:1207	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	48	theme	molecular	1188:1196	arg1	weight					1198:1203	number-average molecular weight	1173:1203	number-average molecular weight (Mn)	1173:1208	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	5	49	theme	2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9	1095:1128	arg1	ratio					1086:1090	a molar ratio	1078:1090	a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9	1078:1128	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	6	50	theme	permeation	1369:1378	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	50	theme	permeation	1369:1378	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	7	51	theme	%	1759:1759	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	9	52	with	polymers	2045:2052	arg1	mass					2067:2070	a molar mass	2059:2070	a molar mass (indicated by Mw) of 3.3 × 106 g/mol	2059:2107	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	8	53	theme	cytokine	1919:1926	arg1	expression					1950:1959	cytokine (IL-2, -4, and IFN-γ) expression	1919:1959	cytokine (IL-2, -4, and IFN-γ) expression	1919:1959	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	7	54	theme	spectrometry	1502:1513	arg1	findings					1465:1472	The findings	1461:1472	The findings of electron ionization mass spectrometry (EI-MS)	1461:1521	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	9	55	dep	mass	2067:2070	arg1	indicated					2073:2081	indicated	2073:2081	indicated by Mw	2073:2087	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	9	56	theme	3.3	2093:2095	arg1	×					2097:2097	×	2097:2097	×	2097:2097	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	7	57	theme	%	1748:1748	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	58	theme	ionization	1486:1495	arg1	EI-MS					1516:1520	EI-MS	1516:1520	EI-MS	1516:1520	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	7	58	theme	ionization	1486:1495	arg1	spectrometry					1502:1513	electron ionization mass spectrometry	1477:1513	electron ionization mass spectrometry (EI-MS)	1477:1521	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	0	59	from	Alfalfa	85:91	arg1	Polysaccharide					65:78	Polysaccharide	65:78	Polysaccharide from Alfalfa	65:91	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	59	from	Alfalfa	85:91	arg1	Methods					8:14	Extract Methods	0:14	Extract Methods	0:14	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	59	from	Alfalfa	85:91	arg1	Characteristics					27:41	Molecular Characteristics	17:41	Molecular Characteristics	17:41	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	59	from	Alfalfa	85:91	arg1	Bioactivities					48:60	Bioactivities	48:60	Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.)	48:112	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	6	60	theme	molecular	1225:1233	arg1	weight					1235:1240	Z-average molecular weight	1215:1240	Z-average molecular weight (Mz) of APS	1215:1252	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	9	61	theme	106	2099:2101	arg1	×					2097:2097	×	2097:2097	×	2097:2097	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	2	62	theme	molecular	482:490	arg1	characteristics					492:506	molecular characteristics	482:506	molecular characteristics	482:506	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	6	63	theme	molecular	1150:1158	arg1	×					1281:1281	3.30 × 106	1276:1285	3.30 × 106	1276:1285	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	63	theme	molecular	1150:1158	arg1	Mw					1168:1169	Mw	1168:1169	Mw	1168:1169	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	63	theme	molecular	1150:1158	arg1	weight					1160:1165	The weight-average molecular weight	1131:1165	The weight-average molecular weight (Mw)	1131:1170	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	5	64	dep	composed	941:948	arg1	GlcA					1067:1070	GlcA	1067:1070	GlcA	1067:1070	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	6	65	theme	APS	1250:1252	arg1	weight					1235:1240	Z-average molecular weight	1215:1240	Z-average molecular weight (Mz) of APS	1215:1252	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	65	theme	APS	1250:1252	arg1	Mw					1168:1169	Mw	1168:1169	Mw	1168:1169	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	65	theme	APS	1250:1252	arg1	Mn					1206:1207	Mn	1206:1207	Mn	1206:1207	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	65	theme	APS	1250:1252	arg1	weight					1160:1165	The weight-average molecular weight	1131:1165	The weight-average molecular weight (Mw)	1131:1170	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	65	theme	APS	1250:1252	arg1	weight					1198:1203	number-average molecular weight	1173:1203	number-average molecular weight (Mn)	1173:1208	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	65	theme	APS	1250:1252	arg1	×					1281:1281	3.30 × 106	1276:1285	3.30 × 106	1276:1285	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	66	dep	weight	1235:1240	arg1	Mz					1243:1244	Mz	1243:1244	Mz	1243:1244	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	7	67	theme	%	1725:1725	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	9	68	theme	immunopotentiating	2148:2165	arg1	agent					2182:2186	an immunopotentiating pharmaceutical agent	2145:2186	an immunopotentiating pharmaceutical agent	2145:2186	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	5	69	theme	ion	884:886	arg1	IC					904:905	IC	904:905	IC	904:905	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	5	69	theme	ion	884:886	arg1	chromatography					888:901	ion chromatography	884:901	ion chromatography (IC) analysis	884:915	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	3	70	attach	isolated	577:584	arg2	polysaccharides					561:575	The crude polysaccharides	551:575	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods	551:651	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	3	70	attach	isolated	577:584	arg1	alfalfa					591:597	alfalfa	591:597	alfalfa	591:597	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	0	71	theme	Polysaccharide	65:78	arg1	Methods					8:14	Extract Methods	0:14	Extract Methods	0:14	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	71	theme	Polysaccharide	65:78	arg1	Characteristics					27:41	Molecular Characteristics	17:41	Molecular Characteristics	17:41	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	71	theme	Polysaccharide	65:78	arg1	Bioactivities					48:60	Bioactivities	48:60	Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.)	48:112	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	3	72	theme	crude	555:559	arg1	polysaccharides					561:575	The crude polysaccharides	551:575	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods	551:651	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	6	73	theme	light	1429:1433	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	73	theme	light	1429:1433	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	8	74	from	proliferation	1901:1913	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	9	75	with	candidate	2132:2140	arg1	mass					2067:2070	a molar mass	2059:2070	a molar mass (indicated by Mw) of 3.3 × 106 g/mol	2059:2107	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	8	76	theme	cells	1966:1970	arg1	expression					1950:1959	cytokine (IL-2, -4, and IFN-γ) expression	1919:1959	cytokine (IL-2, -4, and IFN-γ) expression	1919:1959	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	76	theme	cells	1966:1970	arg1	proliferation					1901:1913	proliferation	1901:1913	proliferation	1901:1913	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	76	theme	cells	1966:1970	arg1	proliferation					1820:1832	B-cell proliferation	1813:1832	B-cell proliferation	1813:1832	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	76	theme	cells	1966:1970	arg1	secretion					1842:1850	IgM secretion	1838:1850	IgM secretion	1838:1850	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	6	77	theme	index-multiangle	1406:1421	arg1	GPC-RI-MALS					1447:1457	GPC-RI-MALS	1447:1457	GPC-RI-MALS	1447:1457	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	6	77	theme	index-multiangle	1406:1421	arg1	scattering					1435:1444	gel permeation chromatography-refractive index-multiangle laser light scattering	1365:1444	gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS)	1365:1458	The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS).					
31137802	0	78	from	Methods	8:14	arg1	Alfalfa					85:91	Alfalfa	85:91	Alfalfa	85:91	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	0	79	theme	Medicago	94:101	arg1	L					110:110	Medicago sativa L	94:110	Medicago sativa L.	94:111	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	2	80	dep	polysaccharide	428:441	arg1	L.					424:425	Medicago sativa L.	408:425	Medicago sativa L.	408:425	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	0	81	from	Bioactivities	48:60	arg1	Alfalfa					85:91	Alfalfa	85:91	Alfalfa	85:91	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	5	82	theme	galacturonic	1020:1031	arg1	acid					1033:1036	galacturonic acid	1020:1036	galacturonic acid (GalA)	1020:1043	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	5	82	theme	galacturonic	1020:1031	arg1	GalA					1039:1042	GalA	1039:1042	GalA	1039:1042	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	9	83	theme	functional	2191:2200	arg1	food					2202:2205	functional food	2191:2205	functional food	2191:2205	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	4	84	dep	three	801:805	arg1	replicates					807:816	replicates	807:816	replicates	807:816	Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination.					
31137802	7	85	theme	%	1710:1710	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	8	86	theme	IgM	1838:1840	arg1	secretion					1842:1850	IgM secretion	1838:1850	IgM secretion	1838:1850	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	3	87	theme	water	602:606	arg1	extraction					608:617	water extraction	602:617	water extraction	602:617	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	4	88	theme	alfalfa	719:725	arg1	samples					727:733	alfalfa samples	719:733	alfalfa samples	719:733	Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination.					
31137802	2	89	theme	purifying	378:386	arg1	methods					388:394	the extraction and purifying methods	359:394	methods	388:394	The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes.					
31137802	5	90	theme	molar	1080:1084	arg1	ratio					1086:1090	a molar ratio	1078:1090	a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9	1078:1128	The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9.					
31137802	0	91	dep	Bioactivities	48:60	arg1	L					110:110	Medicago sativa L	94:110	Medicago sativa L.	94:111	Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.).					
31137802	8	92	dep	proliferation	1901:1913	arg1	the					1897:1899	the	1897:1899	the	1897:1899	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	1	93	theme	immune	282:287	arg1	cells					289:293	immune cells	282:293	immune cells	282:293	The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear.					
31137802	7	94	theme	molar	1684:1688	arg1	proportions					1690:1700	molar proportions	1684:1700	molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively	1684:1773	The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively.					
31137802	8	95	theme	dose-	1857:1861	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	3	96	theme	precipitation	631:643	arg1	methods					645:651	alcohol precipitation methods	623:651	alcohol precipitation methods	623:651	The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis.					
31137802	9	97	theme	g/mol	2103:2107	arg1	mass					2067:2070	a molar mass	2059:2070	a molar mass (indicated by Mw) of 3.3 × 106 g/mol	2059:2107	Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.					
31137802	8	98	theme	time-dependent	1867:1880	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
31137802	8	99	from	secretion	1842:1850	arg1	manner					1882:1887	a dose- and time-dependent manner	1855:1887	a dose- and time-dependent manner	1855:1887	Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells.					
30298790	2	0	dep	H.	509:510	arg1	pylori					512:517	H. pylori strains from pediatric patients with NUD and PUD	509:566	H. pylori strains from pediatric patients with NUD and PUD	509:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	0	dep	H.	509:510	arg1	strains					519:525	strains	519:525	H. pylori strains from pediatric patients with NUD and PUD	509:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	1	theme	pediatric	532:540	arg1	patients					542:549	pediatric patients	532:549	pediatric patients with NUD and PUD	532:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	2	from	growth	499:504	arg1	patients					542:549	pediatric patients	532:549	pediatric patients with NUD and PUD	532:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	1	3	theme	bacterial	380:388	arg1	epitopes					399:406	bacterial adhesion epitopes	380:406	bacterial adhesion epitopes	380:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	1	3	theme	bacterial	380:388	arg1	structures					351:360	glycan structures	344:360	glycan structures that can serve as bacterial adhesion epitopes	344:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	4	dep	pH.	1319:1321	arg1	highlight					1335:1343	highlight	1335:1343	highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1335:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	5	theme	group	1536:1540	arg1	activity					1550:1557	its specific blood group binding activity	1517:1557	its specific blood group binding activity	1517:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	6	theme	NUD	1144:1146	arg1	strains					1148:1154	NUD strains	1144:1154	NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants	1144:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	7	theme	peptic	182:187	arg1	PUD					204:206	PUD	204:206	PUD	204:206	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	7	theme	peptic	182:187	arg1	disease					195:201	peptic ulcer disease	182:201	peptic ulcer disease (PUD)	182:207	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	8	theme	ulcerogenic	1394:1404	arg1	strains					1416:1422	pediatric ulcerogenic H. pylori strains	1384:1422	pediatric ulcerogenic H. pylori strains	1384:1422	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	9	from	mutants	1214:1220	arg1	strains					1148:1154	NUD strains	1144:1154	NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants	1144:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	10	theme	babA-knockout	1200:1212	arg1	mutants					1214:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	11	theme	Helicobacter	111:122	arg1	infection					131:139	neutral and acidic pH. Helicobacter pylori infection	88:139	neutral and acidic pH. Helicobacter pylori infection	88:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	12	theme	BabA-mediated	1357:1369	arg1	adherence					1371:1379	BabA-mediated adherence	1357:1379	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1357:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	13	theme	adhesion	479:486	arg1	modes					488:492	adhesion modes	479:492	adhesion modes	479:492	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	14	theme	babA	961:964	arg1	strains					975:981	H. pylori babA positive strains	951:981	H. pylori babA positive strains	951:981	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	1	15	theme	glycan	344:349	arg1	epitopes					399:406	bacterial adhesion epitopes	380:406	bacterial adhesion epitopes	380:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	1	15	theme	glycan	344:349	arg1	structures					351:360	glycan structures	344:360	glycan structures that can serve as bacterial adhesion epitopes	344:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	16	theme	points	1429:1434	arg1	adherence					1371:1379	BabA-mediated adherence	1357:1379	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1357:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	17	theme	H.	509:510	arg1	modes					488:492	adhesion modes	479:492	adhesion modes	479:492	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	17	theme	H.	509:510	arg1	growth					499:504	growth	499:504	growth	499:504	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	17	theme	H.	509:510	arg1	ability					470:476	binding ability	462:476	binding ability	462:476	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	3	18	theme	acidic	762:767	arg1	pH					769:770	neutral and acidic pH	750:770	pH	769:770	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	19	theme	positive	1033:1040	arg1	strains					1042:1048	NUD babA positive strains	1024:1048	NUD babA positive strains at acidic pH. Binding to Leb	1024:1077	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	20	theme	non-ulcer	155:163	arg1	NUD					176:178	NUD	176:178	NUD	176:178	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	20	theme	non-ulcer	155:163	arg1	dyspepsia					165:173	non-ulcer dyspepsia	155:173	non-ulcer dyspepsia (NUD)	155:179	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	3	21	theme	neutral	750:756	arg1	pH					769:770	neutral and acidic pH	750:770	pH	769:770	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	22	theme	acidic	1266:1271	arg1	pH					1285:1286	acidic and neutral pH	1266:1286	pH	1285:1286	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	23	theme	study	432:436	arg1	aim					413:415	The aim	409:415	The aim of the present study	409:436	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	24	theme	charged	1470:1476	arg1	structures					1478:1487	charged structures	1470:1487	charged structures at acidic pH, separate from its specific blood group binding activity	1470:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	1	25	dep	H.	248:249	arg1	pylori					251:256	H. pylori	248:256	H. pylori	248:256	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	2	26	theme	gastric	571:577	arg1	mucins					579:584	gastric mucins	571:584	gastric mucins	571:584	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	3	27	theme	NUD	730:732	arg1	strains					734:740	the NUD strains	726:740	the NUD strains	726:740	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	0	28	theme	neutral	88:94	arg1	infection					131:139	neutral and acidic pH. Helicobacter pylori infection	88:139	neutral and acidic pH. Helicobacter pylori infection	88:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	29	theme	acidic	100:105	arg1	infection					131:139	neutral and acidic pH. Helicobacter pylori infection	88:139	neutral and acidic pH. Helicobacter pylori infection	88:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	30	theme	babA	881:884	arg1	strains					895:901	babA positive strains	881:901	babA positive strains	881:901	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	31	theme	BabA-mediated	0:12	arg1	adherence					14:22	BabA-mediated adherence	0:22	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection	0:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	32	theme	pH.	1060:1062	arg1	Binding					1064:1070	acidic pH. Binding	1053:1070	acidic pH. Binding to Leb	1053:1077	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	33	from	pH	1285:1286	arg1	mucins					1248:1253	mucins	1248:1253	mucins	1248:1253	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	34	theme	neutral	1277:1283	arg1	pH					1285:1286	acidic and neutral pH	1266:1286	pH	1285:1286	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	35	theme	Lewis	816:820	arg1	b					822:822	Lewis b	816:822	Lewis b (Leb)	816:828	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	3	35	theme	Lewis	816:820	arg1	Leb					825:827	Leb	825:827	Leb	825:827	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	36	theme	blood	1530:1534	arg1	activity					1550:1557	its specific blood group binding activity	1517:1557	its specific blood group binding activity	1517:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	37	theme	H.	1114:1115	arg1	strains					1124:1130	positive PUD H. pylori strains	1101:1130	positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants	1101:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	38	theme	ulcerogenic	37:47	arg1	strains					59:65	pediatric ulcerogenic H. pylori strains	27:65	pediatric ulcerogenic H. pylori strains	27:65	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	3	39	theme	Sialyl-Lewis	831:842	arg1	SLex					847:850	SLex	847:850	SLex	847:850	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	3	39	theme	Sialyl-Lewis	831:842	arg1	x					844:844	Sialyl-Lewis x	831:844	Sialyl-Lewis x (SLex)	831:851	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	2	40	from	patients	542:549	arg1	modes					488:492	adhesion modes	479:492	adhesion modes	479:492	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	40	from	patients	542:549	arg1	growth					499:504	growth	499:504	growth	499:504	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	40	from	patients	542:549	arg1	ability					470:476	binding ability	462:476	binding ability	462:476	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	1	41	theme	gastric	276:282	arg1	layer					290:294	the gastric mucus layer	272:294	the gastric mucus layer	272:294	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	42	theme	associated	931:940	arg1	strains					942:948	PUD associated strains	927:948	PUD associated strains	927:948	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	43	theme	separate	1503:1510	arg1	pH					1499:1500	acidic pH	1492:1500	acidic pH	1492:1500	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	44	from	infection	131:139	arg1	adherence					14:22	BabA-mediated adherence	0:22	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection	0:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	45	theme	positive	1101:1108	arg1	strains					1124:1130	positive PUD H. pylori strains	1101:1130	positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants	1101:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	46	from	modes	488:492	arg1	patients					542:549	pediatric patients	532:549	pediatric patients with NUD and PUD	532:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	47	theme	neutral	1159:1165	arg1	mutants					1214:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	48	theme	binding	1542:1548	arg1	activity					1550:1557	its specific blood group binding activity	1517:1557	its specific blood group binding activity	1517:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	49	theme	increased	609:617	arg1	capacity					628:635	an increased adhesion capacity	606:635	an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins	606:712	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	1	50	theme	adhesion	390:397	arg1	epitopes					399:406	bacterial adhesion epitopes	380:406	bacterial adhesion epitopes	380:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	1	50	theme	adhesion	390:397	arg1	structures					351:360	glycan structures	344:360	glycan structures that can serve as bacterial adhesion epitopes	344:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	51	theme	pediatric	1384:1392	arg1	strains					1416:1422	pediatric ulcerogenic H. pylori strains	1384:1422	pediatric ulcerogenic H. pylori strains	1384:1422	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	52	theme	H.	654:655	arg1	strains					664:670	pediatric PUD H. pylori strains	640:670	pediatric PUD H. pylori strains	640:670	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	0	53	theme	ulcer	189:193	arg1	PUD					204:206	PUD	204:206	PUD	204:206	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	53	theme	ulcer	189:193	arg1	disease					195:201	peptic ulcer disease	182:201	peptic ulcer disease (PUD)	182:207	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	54	theme	H.	1406:1407	arg1	strains					1416:1422	pediatric ulcerogenic H. pylori strains	1384:1422	pediatric ulcerogenic H. pylori strains	1384:1422	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	55	theme	pediatric	640:648	arg1	strains					664:670	pediatric PUD H. pylori strains	640:670	pediatric PUD H. pylori strains	640:670	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	56	theme	derived	1192:1198	arg1	mutants					1214:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	57	theme	pylori	124:129	arg1	infection					131:139	neutral and acidic pH. Helicobacter pylori infection	88:139	neutral and acidic pH. Helicobacter pylori infection	88:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	58	theme	adherence	1371:1379	arg1	role					1349:1352	the role	1345:1352	the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1345:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	59	with	patients	542:549	arg1	PUD					564:566	PUD	564:566	PUD	564:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	2	59	with	patients	542:549	arg1	NUD					556:558	NUD	556:558	NUD	556:558	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	60	theme	positive	966:973	arg1	strains					975:981	H. pylori babA positive strains	951:981	H. pylori babA positive strains	951:981	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	61	theme	strains	664:670	arg1	capacity					628:635	an increased adhesion capacity	606:635	an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins	606:712	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	62	from	activity	1550:1557	arg1	separate					1503:1510	separate	1503:1510	separate	1503:1510	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	63	theme	babA	1028:1031	arg1	strains					1042:1048	NUD babA positive strains	1024:1048	NUD babA positive strains at acidic pH. Binding to Leb	1024:1077	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	64	theme	gastric	699:705	arg1	mucins					707:712	gastric mucins	699:712	gastric mucins	699:712	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	1	65	theme	structures	351:360	arg1	array					335:339	an array	332:339	an array of glycan structures that can serve as bacterial adhesion epitopes	332:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	3	66	dep	human	675:679	arg1	mucins					707:712	gastric mucins	699:712	gastric mucins	699:712	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	3	67	theme	rhesus	685:690	arg1	monkey					692:697	rhesus monkey	685:697	rhesus monkey	685:697	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	3	68	dep	strains	734:740	arg1	both					742:745	both	742:745	both	742:745	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	4	69	theme	strains	1416:1422	arg1	adherence					1371:1379	BabA-mediated adherence	1357:1379	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1357:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	70	from	ability	470:476	arg1	patients					542:549	pediatric patients	532:549	pediatric patients with NUD and PUD	532:566	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	71	theme	gastric	1004:1010	arg1	mucins					1012:1017	gastric mucins	1004:1017	gastric mucins	1004:1017	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	72	theme	gastric	70:76	arg1	mucins					78:83	gastric mucins	70:83	gastric mucins	70:83	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	73	dep	babA	1096:1099	arg1	had					1222:1224	had	1222:1224	had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1222:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	74	from	pH.	1319:1321	arg1	DNA					1305:1307	DNA	1305:1307	DNA	1305:1307	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	74	from	pH.	1319:1321	arg1	SLex					1296:1299	SLex	1296:1299	SLex	1296:1299	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	75	theme	present	424:430	arg1	study					432:436	the present study	420:436	the present study	420:436	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	76	theme	attenuated	1226:1235	arg1	binding					1237:1243	attenuated binding	1226:1243	attenuated binding	1226:1243	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	77	link	derived	1192:1198	arg1	mutants					1214:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	78	dep	common	914:919	arg1	addition					869:876	addition	869:876	addition	869:876	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	2	79	theme	binding	462:468	arg1	ability					470:476	binding ability	462:476	binding ability	462:476	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	0	80	theme	pH.	107:109	arg1	infection					131:139	neutral and acidic pH. Helicobacter pylori infection	88:139	neutral and acidic pH. Helicobacter pylori infection	88:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	81	theme	acidic	1053:1058	arg1	Binding					1064:1070	acidic pH. Binding	1053:1070	acidic pH. Binding to Leb	1053:1077	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	82	from	Binding	1064:1070	arg1	strains					1042:1048	NUD babA positive strains	1024:1048	NUD babA positive strains at acidic pH. Binding to Leb	1024:1077	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	83	contain	had	1222:1224	arg1	strains					1124:1130	positive PUD H. pylori strains	1101:1130	positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants	1101:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	83	contain	had	1222:1224	arg2	binding					1237:1243	attenuated binding	1226:1243	attenuated binding	1226:1243	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	84	theme	pediatric	27:35	arg1	strains					59:65	pediatric ulcerogenic H. pylori strains	27:65	pediatric ulcerogenic H. pylori strains	27:65	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	85	theme	gastric	230:236	arg1	lymphoma					238:245	gastric lymphoma	230:245	gastric lymphoma	230:245	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	86	theme	positive	886:893	arg1	strains					895:901	babA positive strains	881:901	babA positive strains	881:901	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	87	theme	H.	49:50	arg1	strains					59:65	pediatric ulcerogenic H. pylori strains	27:65	pediatric ulcerogenic H. pylori strains	27:65	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	2	88	dep	ability	470:476	arg1	the					458:460	the	458:460	the	458:460	The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins.					
30298790	4	89	theme	specific	1521:1528	arg1	activity					1550:1557	its specific blood group binding activity	1517:1557	its specific blood group binding activity	1517:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	90	from	role	1441:1444	arg1	adhesion					1458:1465	adhesion	1458:1465	adhesion to charged structures at acidic pH, separate from its specific blood group binding activity	1458:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	0	91	theme	strains	59:65	arg1	adherence					14:22	BabA-mediated adherence	0:22	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection	0:139	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	0	92	dep	H.	49:50	arg1	pylori					52:57	H. pylori	49:57	pediatric ulcerogenic H. pylori strains	27:65	BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH. Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma.					
30298790	4	93	theme	acidic	1492:1497	arg1	pH					1499:1500	acidic pH	1492:1500	acidic pH	1492:1500	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	1	94	theme	mucus	284:288	arg1	layer					290:294	the gastric mucus layer	272:294	the gastric mucus layer	272:294	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	95	theme	PUD	927:929	arg1	strains					942:948	PUD associated strains	927:948	PUD associated strains	927:948	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	96	from	pH	1499:1500	arg1	structures					1478:1487	charged structures	1470:1487	charged structures at acidic pH, separate from its specific blood group binding activity	1470:1557	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	4	97	dep	H.	1406:1407	arg1	pylori					1409:1414	H. pylori	1406:1414	pediatric ulcerogenic H. pylori strains	1384:1422	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
30298790	3	98	theme	adhesion	619:626	arg1	capacity					628:635	an increased adhesion capacity	606:635	an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins	606:712	Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc.					
30298790	1	99	contain	carrying	323:330	arg1	mucins					316:321	mucins	316:321	mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes	316:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	1	99	contain	carrying	323:330	arg2	array					335:339	an array	332:339	an array of glycan structures that can serve as bacterial adhesion epitopes	332:406	H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes.					
30298790	4	100	theme	acidic	1176:1181	arg1	mutants					1214:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	neutral, but not acidic, pH. PUD derived babA-knockout mutants	1159:1220	In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.					
31606564	6	0	theme	component	1179:1187	arg1	analysis					1189:1196	Principal component analysis	1169:1196	Principal component analysis from m/z fingerprinting data	1169:1225	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	8	1	theme	Chinese	1750:1756	arg1	medicines					1758:1766	traditional Chinese medicines	1738:1766	traditional Chinese medicines	1738:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	3	2	theme	glycosidic	752:761	arg1	linkages					763:770	glycosidic linkages	752:770	glycosidic linkages	752:770	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	5	3	used	used	1101:1104	arg2	ratio					1051:1055	characteristic peak area ratio	1026:1055	characteristic peak area ratio of Man/Gal in Smith fingerprinting	1026:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	5	4	from	ratio	1051:1055	arg1	fingerprinting					1077:1090	Smith fingerprinting	1071:1090	Smith fingerprinting	1071:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	7	5	theme	glycosidic	1557:1566	arg1	linkages					1568:1575	minor 1,4 glycosidic linkages	1547:1575	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	7	6	theme	1,6	1512:1514	arg1	linkages					1527:1534	predominant 1,6 glycosidic linkages	1500:1534	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	3	7	theme	skeletal	777:784	arg1	structure					786:794	skeletal structure	777:794	skeletal structure	777:794	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	1	8	theme	polysaccharides	192:206	arg1	differentiation					173:187	the differentiation	169:187	the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP)	169:266	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	7	9	theme	minor	1547:1551	arg1	linkages					1568:1575	minor 1,4 glycosidic linkages	1547:1575	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	6	10	theme	clear	1239:1243	arg1	clustering					1245:1254	clear clustering	1239:1254	clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates	1239:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	5	11	theme	characteristic	1026:1039	arg1	ratio					1051:1055	characteristic peak area ratio	1026:1055	characteristic peak area ratio of Man/Gal in Smith fingerprinting	1026:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	5	12	theme	AEP	1156:1158	arg1	discrimination					1138:1151	direct discrimination	1131:1151	direct discrimination of AEP and AOP	1131:1166	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	0	13	from	Astragalus	89:98	arg1	differentiation					43:57	the differentiation	39:57	the differentiation of polysaccharides from genus Astragalus	39:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	0	13	from	Astragalus	89:98	arg1	polysaccharides					62:76	polysaccharides	62:76	polysaccharides from genus Astragalus	62:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	7	14	theme	acid	1460:1463	arg1	hydrolyzates					1465:1476	mild acid hydrolyzates	1455:1476	mild acid hydrolyzates	1455:1476	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	8	15	from	evaluation	1703:1712	arg1	medicines					1758:1766	traditional Chinese medicines	1738:1766	traditional Chinese medicines	1738:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	5	16	theme	AOP	1164:1166	arg1	discrimination					1138:1151	direct discrimination	1131:1151	direct discrimination of AEP and AOP	1131:1166	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	6	17	theme	mild	1311:1314	arg1	hydrolyzates					1321:1332	mild acid hydrolyzates	1311:1332	mild acid hydrolyzates	1311:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	8	18	from	medicines	1758:1766	arg1	polysaccharides					1717:1731	polysaccharides	1717:1731	polysaccharides from traditional Chinese medicines	1717:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	8	18	from	medicines	1758:1766	arg1	evaluation					1703:1712	the evaluation	1699:1712	the evaluation of polysaccharides from traditional Chinese medicines	1699:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	4	19	from	species	915:921	arg1	coefficients					886:897	the correlation coefficients	870:897	the correlation coefficients from homologous species	870:921	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	4	19	from	species	915:921	arg1	more					928:931	more	928:931	more	928:931	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	1	20	theme	chemical	129:136	arg1	fingerprinting					138:151	chemical fingerprinting	129:151	chemical fingerprinting	129:151	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	7	21	theme	hexose	1431:1436	arg1	glycopolymers					1438:1450	hexose glycopolymers	1431:1450	hexose glycopolymers	1431:1450	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	6	22	theme	oligosaccharides	1291:1306	arg1	changes					1280:1286	changes	1280:1286	changes of oligosaccharides in mild acid hydrolyzates	1280:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	4	23	theme	Similarity	797:806	arg1	analysis					808:815	Similarity analysis	797:815	Similarity analysis from GC-MS	797:826	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	7	24	dep	along	1536:1540	arg1	with					1542:1545	with	1542:1545	with	1542:1545	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	7	25	theme	series	1421:1426	arg1	structures					1405:1414	the structures	1401:1414	the structures of a series of hexose glycopolymers in mild acid hydrolyzates	1401:1476	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	8	26	theme	chemical	1594:1601	arg1	mapping					1658:1664	an interconnected structure mapping	1630:1664	not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines	1621:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	8	26	theme	chemical	1594:1601	arg1	fingerprinting					1603:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	3	27	dep	GC-MS	532:536	arg1	reflected					605:613	reflected	605:613	reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure	605:794	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	8	28	theme	interconnected	1633:1646	arg1	mapping					1658:1664	an interconnected structure mapping	1630:1664	not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines	1621:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	8	28	theme	interconnected	1633:1646	arg1	fingerprinting					1603:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	3	29	theme	corresponding	454:466	arg1	products					477:484	The corresponding digested products	450:484	The corresponding digested products	450:484	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	0	30	theme	Chemical	0:7	arg1	techniques					24:33	Chemical fingerprinting techniques	0:33	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus	0:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	1	31	theme	multiple	275:282	arg1	techniques					323:332	multiple chromatographic and mass spectrometric techniques	275:332	multiple chromatographic and mass spectrometric techniques	275:332	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	1	32	theme	generic	103:109	arg1	strategy					111:118	A generic strategy	101:118	A generic strategy based on chemical fingerprinting	101:151	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	5	33	theme	area	1046:1049	arg1	ratio					1051:1055	characteristic peak area ratio	1026:1055	characteristic peak area ratio of Man/Gal in Smith fingerprinting	1026:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	2	34	theme	polysaccharides	433:447	arg1	depolymerization					413:428	the depolymerization	409:428	the depolymerization of polysaccharides	409:447	Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides.					
31606564	1	35	theme	spectrometric	309:321	arg1	techniques					323:332	multiple chromatographic and mass spectrometric techniques	275:332	multiple chromatographic and mass spectrometric techniques	275:332	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	7	36	theme	methylation	1381:1391	arg1	assays					1393:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	8	37	theme	powerful	1677:1684	arg1	approach					1686:1693	a powerful approach	1675:1693	not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines	1621:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	7	38	theme	ESI--MS/MS	1366:1375	arg1	assays					1393:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	4	39	theme	correlation	874:884	arg1	coefficients					886:897	the correlation coefficients	870:897	the correlation coefficients from homologous species	870:921	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	4	39	theme	correlation	874:884	arg1	more					928:931	more	928:931	more	928:931	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	4	40	theme	homologous	904:913	arg1	species					915:921	homologous species	904:921	homologous species	904:921	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	2	41	theme	mild	348:351	arg1	hydrolysis					358:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides.					
31606564	6	42	theme	AEP	1259:1261	arg1	clustering					1245:1254	clear clustering	1239:1254	clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates	1239:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	7	43	theme	m/z	1361:1363	arg1	assays					1393:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	the accurate m/z, ESI--MS/MS and methylation assays	1348:1398	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	3	44	theme	monosaccharide	723:736	arg1	compositions					738:749	monosaccharide compositions	723:749	monosaccharide compositions	723:749	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	3	44	theme	monosaccharide	723:736	arg1	levels					705:710	the three structural levels	684:710	the three structural levels	684:710	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	8	45	theme	polysaccharides	1717:1731	arg1	evaluation					1703:1712	the evaluation	1699:1712	the evaluation of polysaccharides from traditional Chinese medicines	1699:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	2	46	theme	Smith	373:377	arg1	degradation					379:389	Smith degradation	373:389	Smith degradation	373:389	Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides.					
31606564	6	47	theme	Principal	1169:1177	arg1	analysis					1189:1196	Principal component analysis	1169:1196	Principal component analysis from m/z fingerprinting data	1169:1225	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	8	48	theme	traditional	1738:1748	arg1	medicines					1758:1766	traditional Chinese medicines	1738:1766	traditional Chinese medicines	1738:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	2	49	theme	Complete	335:342	arg1	hydrolysis					358:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides.					
31606564	7	50	theme	1,4	1553:1555	arg1	linkages					1568:1575	minor 1,4 glycosidic linkages	1547:1575	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	4	51	theme	heterologous	964:975	arg1	species					977:983	heterologous species	964:983	heterologous species	964:983	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	6	52	theme	AOP	1267:1269	arg1	clustering					1245:1254	clear clustering	1239:1254	clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates	1239:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	3	53	theme	bottom-up	580:588	arg1	fingerprinting					590:603	The resulting bottom-up fingerprinting	566:603	The resulting bottom-up fingerprinting	566:603	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	7	54	theme	glycosidic	1516:1525	arg1	linkages					1527:1534	predominant 1,6 glycosidic linkages	1500:1534	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	3	55	theme	native	633:638	arg1	polysaccharides					640:654	native polysaccharides	633:654	native polysaccharides	633:654	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	7	56	theme	predominant	1500:1510	arg1	linkages					1527:1534	predominant 1,6 glycosidic linkages	1500:1534	predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages	1500:1575	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	6	57	theme	acid	1316:1319	arg1	hydrolyzates					1321:1332	mild acid hydrolyzates	1311:1332	mild acid hydrolyzates	1311:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	7	58	theme	mild	1455:1458	arg1	hydrolyzates					1465:1476	mild acid hydrolyzates	1455:1476	mild acid hydrolyzates	1455:1476	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	3	59	from	variations	619:628	arg1	polysaccharides					640:654	native polysaccharides	633:654	native polysaccharides	633:654	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	5	60	theme	direct	1131:1136	arg1	discrimination					1138:1151	direct discrimination	1131:1151	direct discrimination of AEP and AOP	1131:1166	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	6	61	from	data	1222:1225	arg1	analysis					1189:1196	Principal component analysis	1169:1196	Principal component analysis from m/z fingerprinting data	1169:1225	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	8	62	theme	established	1582:1592	arg1	mapping					1658:1664	an interconnected structure mapping	1630:1664	not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines	1621:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	8	62	theme	established	1582:1592	arg1	fingerprinting					1603:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	7	63	theme	glycopolymers	1438:1450	arg1	series					1421:1426	a series	1419:1426	a series of hexose glycopolymers	1419:1450	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	6	64	from	hydrolyzates	1321:1332	arg1	changes					1280:1286	changes	1280:1286	changes of oligosaccharides in mild acid hydrolyzates	1280:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	7	65	theme	accurate	1352:1359	arg1	m/z					1361:1363	the accurate m/z	1348:1363	the accurate m/z	1348:1363	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	0	66	theme	genus	83:87	arg1	Astragalus					89:98	genus Astragalus	83:98	genus Astragalus	83:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	1	67	from	Astragalus	213:222	arg1	polysaccharides					192:206	polysaccharides	192:206	polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP)	192:266	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	1	67	from	Astragalus	213:222	arg1	differentiation					173:187	the differentiation	169:187	the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP)	169:266	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	6	68	from	changes	1280:1286	arg1	hydrolyzates					1321:1332	mild acid hydrolyzates	1311:1332	mild acid hydrolyzates	1311:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	6	69	from	oligosaccharides	1291:1306	arg1	hydrolyzates					1321:1332	mild acid hydrolyzates	1311:1332	mild acid hydrolyzates	1311:1332	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	1	70	from	differentiation	173:187	arg1	Astragalus					213:222	Astragalus	213:222	Astragalus membranaceus (AEP) and A. mongholicus (AOP)	213:266	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	4	71	theme	HILIC-ELSD	832:841	arg1	fingerprinting					843:856	HILIC-ELSD fingerprinting	832:856	HILIC-ELSD fingerprinting	832:856	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	6	72	theme	m/z	1203:1205	arg1	data					1222:1225	m/z fingerprinting data	1203:1225	m/z fingerprinting data	1203:1225	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	5	73	theme	Man/Gal	1060:1066	arg1	ratio					1051:1055	characteristic peak area ratio	1026:1055	characteristic peak area ratio of Man/Gal in Smith fingerprinting	1026:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	0	74	from	differentiation	43:57	arg1	Astragalus					89:98	genus Astragalus	83:98	genus Astragalus	83:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	0	75	theme	fingerprinting	9:22	arg1	techniques					24:33	Chemical fingerprinting techniques	0:33	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus	0:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	4	76	from	GC-MS	822:826	arg1	fingerprinting					843:856	HILIC-ELSD fingerprinting	832:856	HILIC-ELSD fingerprinting	832:856	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	4	76	from	GC-MS	822:826	arg1	analysis					808:815	Similarity analysis	797:815	Similarity analysis from GC-MS	797:826	Similarity analysis from GC-MS and HILIC-ELSD fingerprinting showed that the correlation coefficients from homologous species were more than 0.914, whereas those from heterologous species were less than 0.796.					
31606564	3	77	theme	digested	468:475	arg1	products					477:484	The corresponding digested products	450:484	The corresponding digested products	450:484	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	1	78	theme	chromatographic	284:298	arg1	techniques					323:332	multiple chromatographic and mass spectrometric techniques	275:332	multiple chromatographic and mass spectrometric techniques	275:332	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	5	79	theme	peak	1041:1044	arg1	ratio					1051:1055	characteristic peak area ratio	1026:1055	characteristic peak area ratio of Man/Gal in Smith fingerprinting	1026:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	1	80	theme	mass	304:307	arg1	techniques					323:332	multiple chromatographic and mass spectrometric techniques	275:332	multiple chromatographic and mass spectrometric techniques	275:332	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	3	81	theme	structural	694:703	arg1	compositions					738:749	monosaccharide compositions	723:749	monosaccharide compositions	723:749	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	3	81	theme	structural	694:703	arg1	levels					705:710	the three structural levels	684:710	the three structural levels	684:710	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
31606564	1	82	dep	Astragalus	213:222	arg1	mongholicus					250:260	A. mongholicus	247:260	A. mongholicus (AOP)	247:266	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	1	82	dep	Astragalus	213:222	arg1	AEP					238:240	AEP	238:240	AEP	238:240	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	1	82	dep	Astragalus	213:222	arg1	AOP					263:265	AOP	263:265	AOP	263:265	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	1	82	dep	Astragalus	213:222	arg1	membranaceus					224:235	membranaceus	224:235	membranaceus (AEP)	224:241	A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques.					
31606564	0	83	theme	polysaccharides	62:76	arg1	differentiation					43:57	the differentiation	39:57	the differentiation of polysaccharides from genus Astragalus	39:98	Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus.					
31606564	5	84	theme	feasible	1108:1115	arg1	parameter					1117:1125	a feasible parameter	1106:1125	a feasible parameter for direct discrimination of AEP and AOP	1106:1166	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	8	85	theme	structure	1648:1656	arg1	mapping					1658:1664	an interconnected structure mapping	1630:1664	not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines	1621:1766	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	8	85	theme	structure	1648:1656	arg1	fingerprinting					1603:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting	1578:1616	The established chemical fingerprinting is not only an interconnected structure mapping but also a powerful approach for the evaluation of polysaccharides from traditional Chinese medicines.					
31606564	2	86	theme	acid	353:356	arg1	hydrolysis					358:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis	335:367	Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides.					
31606564	5	87	theme	Smith	1071:1075	arg1	fingerprinting					1077:1090	Smith fingerprinting	1071:1090	Smith fingerprinting	1071:1090	It also noted that characteristic peak area ratio of Man/Gal in Smith fingerprinting could be used a feasible parameter for direct discrimination of AEP and AOP.					
31606564	6	88	theme	fingerprinting	1207:1220	arg1	data					1222:1225	m/z fingerprinting data	1203:1225	m/z fingerprinting data	1203:1225	Principal component analysis from m/z fingerprinting data resulted in clear clustering of AEP and AOP based on changes of oligosaccharides in mild acid hydrolyzates.					
31606564	7	89	from	structures	1405:1414	arg1	hydrolyzates					1465:1476	mild acid hydrolyzates	1455:1476	mild acid hydrolyzates	1455:1476	By combining the accurate m/z, ESI--MS/MS and methylation assays, the structures of a series of hexose glycopolymers in mild acid hydrolyzates were characterized by predominant 1,6 glycosidic linkages along with minor 1,4 glycosidic linkages.					
31606564	3	90	theme	resulting	570:578	arg1	fingerprinting					590:603	The resulting bottom-up fingerprinting	566:603	The resulting bottom-up fingerprinting	566:603	The corresponding digested products were efficiently separated and detected using GC-MS, HILIC-ELSD and HR-ESI--MS. The resulting bottom-up fingerprinting reflected the variations in native polysaccharides, which may be attributed to the three structural levels, which are monosaccharide compositions, glycosidic linkages, and skeletal structure.					
30389790	4	0	from	Δgls1-S	890:896	arg1	G3M9					882:885	G3M9	882:885	G3M9	882:885	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	0	from	Δgls1-S	890:896	arg1	glycans					869:875	the most abundantly formed glycans	842:875	the most abundantly formed glycans	842:875	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	1	theme	strains	820:826	arg1	patterns					800:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns	741:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	741:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	10	2	theme	Δgls1-S	1838:1844	arg1	cells					1846:1850	Δgls1-S cells	1838:1850	Δgls1-S cells	1838:1850	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	7	3	theme	reduced	1410:1416	arg1	degradation					1430:1440	reduced proteasomal degradation	1410:1440	reduced proteasomal degradation of misfolded glycoproteins	1410:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	10	4	theme	human	1780:1784	arg1	endomannosidase					1786:1800	human endomannosidase	1780:1800	human endomannosidase	1780:1800	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	1	5	theme	endoplasmic	251:261	arg1	ER					274:275	ER	274:275	ER	274:275	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	1	5	theme	endoplasmic	251:261	arg1	reticulum					263:271	the endoplasmic reticulum	247:271	the endoplasmic reticulum (ER)	247:276	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	7	6	theme	glycoproteins	1455:1467	arg1	degradation					1430:1440	reduced proteasomal degradation	1410:1440	reduced proteasomal degradation of misfolded glycoproteins	1410:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	6	theme	glycoproteins	1455:1467	arg1	inhibition					1332:1341	inhibition	1332:1341	inhibition of the oligosaccharyltransferase by transfer reaction products	1332:1404	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	6	theme	glycoproteins	1455:1467	arg1	disruption					1266:1275	disruption	1266:1275	disruption of glycoprotein entrance into calnexin-folding cycles	1266:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	4	7	theme	lipid-linked	771:782	arg1	oligosaccharide					784:798	The lipid-linked oligosaccharide	767:798	The lipid-linked oligosaccharide	767:798	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	3	8	theme	Δgls1	514:518	arg1	mutants					520:526	two viable Δgls1 mutants	503:526	two viable Δgls1 mutants	503:526	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	8	theme	Δgls1	514:518	arg1	other					593:597	other	593:597	other	593:597	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	8	theme	Δgls1	514:518	arg1	one					529:531	one	529:531	one	529:531	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	7	9	theme	reaction	1388:1395	arg1	products					1397:1404	transfer reaction products	1379:1404	transfer reaction products	1379:1404	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	1	10	link	protein-linked	206:219	arg1	G3M9					238:241	G3M9	238:241	G3M9	238:241	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	1	10	link	protein-linked	206:219	arg1	Glc3Man9GlcNAc2					221:235	protein-linked Glc3Man9GlcNAc2	206:235	protein-linked Glc3Man9GlcNAc2 (G3M9)	206:242	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	10	11	theme	normal	1821:1826	arg1	growth					1828:1833	normal growth	1821:1833	normal growth in Δgls1-S cells	1821:1850	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	4	12	theme	formed	862:867	arg1	G3M9					882:885	G3M9	882:885	G3M9	882:885	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	12	theme	formed	862:867	arg1	glycans					869:875	the most abundantly formed glycans	842:875	the most abundantly formed glycans	842:875	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	3	13	with	other	593:597	arg1	Δgls1-H					621:627	Δgls1-H	621:627	Δgls1-H	621:627	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	13	with	other	593:597	arg1	one					616:618	a healthier one	604:618	a healthier one (Δgls1-H)	604:628	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	1	14	theme	outermost	183:191	arg1	glucose					193:199	the outermost glucose	179:199	the outermost glucose	179:199	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	9	15	theme	ER	1733:1734	arg1	membranes					1736:1744	underlying ER membranes	1722:1744	underlying ER membranes	1722:1744	Nevertheless, it resulted in a distorted cell wall and in the absence of underlying ER membranes.					
30389790	0	16	from	phenotype	84:92	arg1	yeasts					105:110	fission yeasts	97:110	fission yeasts: Model for the human MOGS-CDG disorder	97:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	0	17	theme	human	127:131	arg1	disorder					142:149	the human MOGS-CDG disorder	123:149	the human MOGS-CDG disorder	123:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	5	18	theme	alg10+	1026:1031	arg1	gene					1033:1036	the alg10+ gene	1022:1036	the alg10+ gene	1022:1036	A mutation in the alg10+ gene was indeed observed in this strain.					
30389790	3	19	theme	lethal	558:563	arg1	Δgls1-S					576:582	Δgls1-S	576:582	Δgls1-S	576:582	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	19	theme	lethal	558:563	arg1	phenotype					565:573	a very sick but not lethal phenotype	538:573	a very sick but not lethal phenotype (Δgls1-S)	538:583	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	9	20	theme	cell	1690:1693	arg1	wall					1695:1698	a distorted cell wall	1678:1698	a distorted cell wall	1678:1698	Nevertheless, it resulted in a distorted cell wall and in the absence of underlying ER membranes.					
30389790	11	21	gly	glycoproteins	1898:1910	arg1	glycoproteins					1898:1910	G3M9-bearing glycoproteins	1885:1910	G3M9-bearing glycoproteins	1885:1910	We propose that accumulation of G3M9-bearing glycoproteins is toxic and at least partially responsible for defects observed in MOGS-CDG.					
30389790	5	22	from	mutation	1010:1017	arg1	gene					1033:1036	the alg10+ gene	1022:1036	the alg10+ gene	1022:1036	A mutation in the alg10+ gene was indeed observed in this strain.					
30389790	8	23	theme	glycoprotein	1494:1505	arg1	Lack					1470:1473	Lack	1470:1473	Lack of triglucosylated glycoprotein	1470:1505	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	5	24	located	observed	1049:1056	arg1	strain					1066:1071	this strain	1061:1071	this strain	1061:1071	A mutation in the alg10+ gene was indeed observed in this strain.					
30389790	5	24	located	observed	1049:1056	arg2	mutation					1010:1017	A mutation	1008:1017	A mutation in the alg10+ gene	1008:1036	A mutation in the alg10+ gene was indeed observed in this strain.					
30389790	10	25	from	growth	1828:1833	arg1	cells					1846:1850	Δgls1-S cells	1838:1850	Δgls1-S cells	1838:1850	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	0	26	theme	fission	97:103	arg1	yeasts					105:110	fission yeasts	97:110	fission yeasts: Model for the human MOGS-CDG disorder	97:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	2	27	contain	bear	343:346	arg1	Individuals					279:289	Individuals	279:289	Individuals with congenital disorders of glycosylation MOGS-CDG	279:341	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	2	27	contain	bear	343:346	arg2	mutations					348:356	mutations	348:356	mutations in the GI-encoding gene (gls1)	348:387	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	7	28	gly	glycoproteins	1455:1467	arg1	glycoproteins					1455:1467	misfolded glycoproteins	1445:1467	misfolded glycoproteins	1445:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	4	29	from	G3M9	882:885	arg1	Δgls1-S					890:896	Δgls1-S	890:896	Δgls1-S	890:896	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	29	from	G3M9	882:885	arg1	Δgls1-H					921:927	Δgls1-H	921:927	Δgls1-H	921:927	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	29	from	G3M9	882:885	arg1	Glc2Man9GlcNAc2					902:916	Glc2Man9GlcNAc2	902:916	Glc2Man9GlcNAc2	902:916	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	7	30	gly	glycoprotein	1280:1291	arg1	glycoprotein					1280:1291	glycoprotein entrance	1280:1300	glycoprotein entrance into calnexin-folding cycles	1280:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	31	theme	entrance	1293:1300	arg1	degradation					1430:1440	reduced proteasomal degradation	1410:1440	reduced proteasomal degradation of misfolded glycoproteins	1410:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	31	theme	entrance	1293:1300	arg1	inhibition					1332:1341	inhibition	1332:1341	inhibition of the oligosaccharyltransferase by transfer reaction products	1332:1404	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	31	theme	entrance	1293:1300	arg1	disruption					1266:1275	disruption	1266:1275	disruption of glycoprotein entrance into calnexin-folding cycles	1266:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	4	32	theme	Δgls1-H	992:998	arg1	strain					1000:1005	the Δgls1-H strain	988:1005	the Δgls1-H strain	988:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	33	theme	ER	673:674	arg1	oligosaccharide					691:705	an ER protein-linked oligosaccharide	670:705	an ER protein-linked oligosaccharide	670:705	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	33	theme	ER	673:674	arg1	G3M9					662:665	only G3M9	657:665	only G3M9	657:665	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	0	34	theme	glucosidase	14:24	arg1	deglucosylation					50:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	4	35	theme	reduced	941:947	arg1	activity					976:983	reduced Alg10p glucosyltransferase activity	941:983	reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	941:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	36	theme	G3M9	746:749	arg1	patterns					800:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns	741:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	741:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	0	37	theme	glycoprotein	37:48	arg1	deglucosylation					50:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	4	38	theme	glucosyltransferase	956:974	arg1	activity					976:983	reduced Alg10p glucosyltransferase activity	941:983	reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	941:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	39	theme	Man9GlcNAc2	755:765	arg1	patterns					800:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns	741:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	741:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	2	40	theme	MOGS-CDG	334:341	arg1	disorders					307:315	congenital disorders	296:315	congenital disorders of glycosylation MOGS-CDG	296:341	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	8	41	theme	cell	1611:1614	arg1	composition					1636:1646	the overall cell wall monosaccharide composition	1599:1646	the overall cell wall monosaccharide composition	1599:1646	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	4	42	theme	healthier	720:728	arg1	strain					730:735	the healthier strain	716:735	the healthier strain	716:735	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	3	43	theme	Schizosaccharomyces	452:470	arg1	yeasts					478:483	Schizosaccharomyces pombe yeasts	452:483	Schizosaccharomyces pombe yeasts	452:483	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	1	44	theme	protein-linked	206:219	arg1	G3M9					238:241	G3M9	238:241	G3M9	238:241	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	1	44	theme	protein-linked	206:219	arg1	Glc3Man9GlcNAc2					221:235	protein-linked Glc3Man9GlcNAc2	206:235	protein-linked Glc3Man9GlcNAc2 (G3M9)	206:242	Glucosidase I (GI) removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 (G3M9) in the endoplasmic reticulum (ER).					
30389790	4	45	link	protein-linked	676:689	arg1	oligosaccharide					691:705	an ER protein-linked oligosaccharide	670:705	an ER protein-linked oligosaccharide	670:705	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	45	link	protein-linked	676:689	arg1	G3M9					662:665	only G3M9	657:665	only G3M9	657:665	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	8	46	theme	monosaccharide	1621:1634	arg1	composition					1636:1646	the overall cell wall monosaccharide composition	1599:1646	the overall cell wall monosaccharide composition	1599:1646	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	3	47	theme	healthier	606:614	arg1	Δgls1-H					621:627	Δgls1-H	621:627	Δgls1-H	621:627	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	47	theme	healthier	606:614	arg1	one					616:618	a healthier one	604:618	a healthier one (Δgls1-H)	604:628	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	10	48	theme	Golgi	1760:1764	arg1	expression					1766:1775	Golgi expression	1760:1775	Golgi expression of human endomannosidase	1760:1800	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	9	49	theme	distorted	1680:1688	arg1	wall					1695:1698	a distorted cell wall	1678:1698	a distorted cell wall	1678:1698	Nevertheless, it resulted in a distorted cell wall and in the absence of underlying ER membranes.					
30389790	7	50	theme	Further	1198:1204	arg1	studies					1206:1212	Further studies	1198:1212	Further studies	1198:1212	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	10	51	theme	endomannosidase	1786:1800	arg1	expression					1766:1775	Golgi expression	1760:1775	Golgi expression of human endomannosidase	1760:1800	Furthermore, Golgi expression of human endomannosidase partially restored normal growth in Δgls1-S cells.					
30389790	6	52	theme	abrogated	1101:1109	arg1	deglucosylation					1116:1130	abrogated G3M9 deglucosylation	1101:1130	abrogated G3M9 deglucosylation	1101:1130	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	7	53	theme	misfolded	1445:1453	arg1	glycoproteins					1455:1467	misfolded glycoproteins	1445:1467	misfolded glycoproteins	1445:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	54	theme	proteasomal	1418:1428	arg1	degradation					1430:1440	reduced proteasomal degradation	1410:1440	reduced proteasomal degradation of misfolded glycoproteins	1410:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	11	55	theme	glycoproteins	1898:1910	arg1	accumulation					1869:1880	accumulation	1869:1880	accumulation of G3M9-bearing glycoproteins	1869:1910	We propose that accumulation of G3M9-bearing glycoproteins is toxic and at least partially responsible for defects observed in MOGS-CDG.					
30389790	3	56	theme	viable	507:512	arg1	mutants					520:526	two viable Δgls1 mutants	503:526	two viable Δgls1 mutants	503:526	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	56	theme	viable	507:512	arg1	other					593:597	other	593:597	other	593:597	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	56	theme	viable	507:512	arg1	one					529:531	one	529:531	one	529:531	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	2	57	from	mutations	348:356	arg1	gls1					383:386	gls1	383:386	gls1	383:386	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	2	57	from	mutations	348:356	arg1	gene					377:380	the GI-encoding gene	361:380	the GI-encoding gene (gls1)	361:387	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	6	58	theme	G3M9	1111:1114	arg1	deglucosylation					1116:1130	abrogated G3M9 deglucosylation	1101:1130	abrogated G3M9 deglucosylation	1101:1130	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	6	59	located	observed	1171:1178	arg2	defects					1163:1169	the severe defects	1152:1169	the severe defects observed in Δgls1-S cells	1152:1195	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	6	59	located	observed	1171:1178	arg1	cells					1191:1195	Δgls1-S cells	1183:1195	Δgls1-S cells	1183:1195	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	2	60	theme	congenital	296:305	arg1	disorders					307:315	congenital disorders	296:315	congenital disorders of glycosylation MOGS-CDG	296:341	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	9	61	theme	underlying	1722:1731	arg1	membranes					1736:1744	underlying ER membranes	1722:1744	underlying ER membranes	1722:1744	Nevertheless, it resulted in a distorted cell wall and in the absence of underlying ER membranes.					
30389790	7	62	theme	transfer	1379:1386	arg1	products					1397:1404	transfer reaction products	1379:1404	transfer reaction products	1379:1404	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	0	63	theme	MOGS-CDG	133:140	arg1	disorder					142:149	the human MOGS-CDG disorder	123:149	the human MOGS-CDG disorder	123:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	4	64	dep	G3M9	746:749	arg1	oligosaccharide					784:798	The lipid-linked oligosaccharide	767:798	The lipid-linked oligosaccharide	767:798	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	9	65	theme	membranes	1736:1744	arg1	absence					1711:1717	the absence	1707:1717	the absence of underlying ER membranes	1707:1744	Nevertheless, it resulted in a distorted cell wall and in the absence of underlying ER membranes.					
30389790	7	66	theme	oligosaccharyltransferase	1350:1374	arg1	degradation					1430:1440	reduced proteasomal degradation	1410:1440	reduced proteasomal degradation of misfolded glycoproteins	1410:1467	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	66	theme	oligosaccharyltransferase	1350:1374	arg1	inhibition					1332:1341	inhibition	1332:1341	inhibition of the oligosaccharyltransferase by transfer reaction products	1332:1404	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	7	66	theme	oligosaccharyltransferase	1350:1374	arg1	disruption					1266:1275	disruption	1266:1275	disruption of glycoprotein entrance into calnexin-folding cycles	1266:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	4	67	link	lipid-linked	771:782	arg1	oligosaccharide					784:798	The lipid-linked oligosaccharide	767:798	The lipid-linked oligosaccharide	767:798	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	3	68	theme	sick	545:548	arg1	Δgls1-S					576:582	Δgls1-S	576:582	Δgls1-S	576:582	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	3	68	theme	sick	545:548	arg1	phenotype					565:573	a very sick but not lethal phenotype	538:573	a very sick but not lethal phenotype (Δgls1-S)	538:583	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	8	69	gly	glycoprotein	1494:1505	arg1	glycoprotein					1494:1505	triglucosylated glycoprotein	1478:1505	triglucosylated glycoprotein	1478:1505	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	4	70	theme	sick	635:638	arg1	strain					640:645	The sick strain	631:645	The sick strain	631:645	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	8	71	theme	triglucosylated	1478:1492	arg1	glycoprotein					1494:1505	triglucosylated glycoprotein	1478:1505	triglucosylated glycoprotein	1478:1505	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	6	72	theme	severe	1156:1161	arg1	defects					1163:1169	the severe defects	1152:1169	the severe defects observed in Δgls1-S cells	1152:1195	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	4	73	contain	had	737:739	arg1	strain					730:735	the healthier strain	716:735	the healthier strain	716:735	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	73	contain	had	737:739	arg2	patterns					800:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns	741:807	both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	741:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	0	74	theme	sick	79:82	arg1	phenotype					84:92	a sick phenotype	77:92	a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder	77:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	7	75	theme	calnexin-folding	1307:1322	arg1	cycles					1324:1329	calnexin-folding cycles	1307:1329	calnexin-folding cycles	1307:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	2	76	with	Individuals	279:289	arg1	disorders					307:315	congenital disorders	296:315	congenital disorders of glycosylation MOGS-CDG	296:341	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	8	77	theme	glycan	1555:1560	arg1	elongation					1562:1571	glycan elongation	1555:1571	glycan elongation	1555:1571	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	4	78	theme	protein-linked	676:689	arg1	oligosaccharide					691:705	an ER protein-linked oligosaccharide	670:705	an ER protein-linked oligosaccharide	670:705	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	78	theme	protein-linked	676:689	arg1	G3M9					662:665	only G3M9	657:665	only G3M9	657:665	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	11	79	theme	G3M9-bearing	1885:1896	arg1	glycoproteins					1898:1910	G3M9-bearing glycoproteins	1885:1910	G3M9-bearing glycoproteins	1885:1910	We propose that accumulation of G3M9-bearing glycoproteins is toxic and at least partially responsible for defects observed in MOGS-CDG.					
30389790	0	80	dep	yeasts	105:110	arg1	Model					113:117	Model	113:117	fission yeasts: Model for the human MOGS-CDG disorder	97:149	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	7	81	theme	glycoprotein	1280:1291	arg1	entrance					1293:1300	glycoprotein entrance	1280:1300	glycoprotein entrance into calnexin-folding cycles	1280:1329	Further studies disclosed that the defects could not be ascribed to disruption of glycoprotein entrance into calnexin-folding cycles, inhibition of the oligosaccharyltransferase by transfer reaction products, or reduced proteasomal degradation of misfolded glycoproteins.					
30389790	2	82	theme	GI-encoding	365:375	arg1	gls1					383:386	gls1	383:386	gls1	383:386	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	2	82	theme	GI-encoding	365:375	arg1	gene					377:380	the GI-encoding gene	361:380	the GI-encoding gene (gls1)	361:387	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	0	83	theme	I-mediated	26:35	arg1	deglucosylation					50:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	0	84	theme	deglucosylation	50:64	arg1	Abrogation					0:9	Abrogation	0:9	Abrogation of glucosidase I-mediated glycoprotein deglucosylation	0:64	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	4	85	from	activity	976:983	arg1	strain					1000:1005	the Δgls1-H strain	988:1005	the Δgls1-H strain	988:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	3	86	theme	GI	399:400	arg1	absence					402:408	GI absence	399:408	GI absence	399:408	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	8	87	theme	overall	1603:1609	arg1	composition					1636:1646	the overall cell wall monosaccharide composition	1599:1646	the overall cell wall monosaccharide composition	1599:1646	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	11	88	located	observed	1968:1975	arg2	defects					1960:1966	defects	1960:1966	defects observed in MOGS-CDG	1960:1987	We propose that accumulation of G3M9-bearing glycoproteins is toxic and at least partially responsible for defects observed in MOGS-CDG.					
30389790	11	88	located	observed	1968:1975	arg1	MOGS-CDG					1980:1987	MOGS-CDG	1980:1987	MOGS-CDG	1980:1987	We propose that accumulation of G3M9-bearing glycoproteins is toxic and at least partially responsible for defects observed in MOGS-CDG.					
30389790	4	89	theme	Alg10p	949:954	arg1	activity					976:983	reduced Alg10p glucosyltransferase activity	941:983	reduced Alg10p glucosyltransferase activity in the Δgls1-H strain	941:1005	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	8	90	theme	wall	1616:1619	arg1	composition					1636:1646	the overall cell wall monosaccharide composition	1599:1646	the overall cell wall monosaccharide composition	1599:1646	Lack of triglucosylated glycoprotein deglucosylation neither significantly prevented glycan elongation in the Golgi nor modified the overall cell wall monosaccharide composition.					
30389790	3	91	from	lethality	439:447	arg1	yeasts					478:483	Schizosaccharomyces pombe yeasts	452:483	Schizosaccharomyces pombe yeasts	452:483	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	2	92	theme	glycosylation	320:332	arg1	MOGS-CDG					334:341	glycosylation MOGS-CDG	320:341	glycosylation MOGS-CDG	320:341	Individuals with congenital disorders of glycosylation MOGS-CDG bear mutations in the GI-encoding gene (gls1).					
30389790	0	93	gly	glycoprotein	37:48	arg1	glycoprotein					37:48	glucosidase I-mediated glycoprotein deglucosylation	14:64	glucosidase I-mediated glycoprotein deglucosylation	14:64	Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.					
30389790	6	94	theme	Δgls1-S	1183:1189	arg1	cells					1191:1195	Δgls1-S cells	1183:1195	Δgls1-S cells	1183:1195	Our results indicated that abrogated G3M9 deglucosylation was responsible for the severe defects observed in Δgls1-S cells.					
30389790	3	95	theme	pombe	472:476	arg1	yeasts					478:483	Schizosaccharomyces pombe yeasts	452:483	Schizosaccharomyces pombe yeasts	452:483	Although GI absence has been reported to produce lethality in Schizosaccharomyces pombe yeasts, here we obtained two viable Δgls1 mutants, one with a very sick but not lethal phenotype (Δgls1-S) and the other with a healthier one (Δgls1-H).					
30389790	4	96	from	Δgls1-H	921:927	arg1	G3M9					882:885	G3M9	882:885	G3M9	882:885	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	96	from	Δgls1-H	921:927	arg1	glycans					869:875	the most abundantly formed glycans	842:875	the most abundantly formed glycans	842:875	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	97	from	Glc2Man9GlcNAc2	902:916	arg1	G3M9					882:885	G3M9	882:885	G3M9	882:885	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
30389790	4	97	from	Glc2Man9GlcNAc2	902:916	arg1	glycans					869:875	the most abundantly formed glycans	842:875	the most abundantly formed glycans	842:875	The sick strain displayed only G3M9 as an ER protein-linked oligosaccharide, whereas the healthier strain had both G3M9 and Man9GlcNAc2 The lipid-linked oligosaccharide patterns of the two strains revealed that the most abundantly formed glycans were G3M9 in Δgls1-S and Glc2Man9GlcNAc2 in Δgls1-H, suggesting reduced Alg10p glucosyltransferase activity in the Δgls1-H strain.					
29880818	11	0	theme	direct	1297:1302	arg1	FTX					1287:1289	LncRNA FTX	1280:1289	LncRNA FTX	1280:1289	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	11	0	theme	direct	1297:1302	arg1	target					1304:1309	a direct target	1295:1309	a direct target of miR-342	1295:1320	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	3	1	from	players	373:379	arg1	development					388:398	the development	384:398	the development of AML resistance to chemotherapy	384:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	8	2	theme	N-glycan	956:963	arg1	alteration					929:938	the alteration	925:938	the alteration of high-mannose N-glycan	925:963	Importantly, the alteration of high-mannose N-glycan was further confirmed by detecting the enzyme level of ALG family.					
29880818	1	3	theme	acute	167:171	arg1	AML					191:193	AML	191:193	AML	191:193	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	1	3	theme	acute	167:171	arg1	leukemia					181:188	acute myeloid leukemia	167:188	acute myeloid leukemia (AML) chemotherapy	167:207	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	4	4	theme	present	442:448	arg1	study					450:454	the present study	438:454	the present study	438:454	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	4	5	theme	-resistant	535:544	arg1	cells					555:559	adriamycin (ADR)-resistant U937/ADR cells	519:559	adriamycin (ADR)-resistant U937/ADR cells	519:559	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	0	6	theme	resistance	91:100	arg1	development					71:81	development	71:81	development of drug resistance in acute myeloid leukemia	71:126	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	11	7	theme	LncRNA	1280:1285	arg1	FTX					1287:1289	LncRNA FTX	1280:1289	LncRNA FTX	1280:1289	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	11	7	theme	LncRNA	1280:1285	arg1	target					1304:1309	a direct target	1295:1309	a direct target of miR-342	1295:1320	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	6	8	theme	proteins	776:783	arg1	binding					756:762	the strong binding	745:762	the strong binding of membrane proteins	745:783	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	1	9	theme	myeloid	173:179	arg1	AML					191:193	AML	191:193	AML	191:193	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	1	9	theme	myeloid	173:179	arg1	leukemia					181:188	acute myeloid leukemia	167:188	acute myeloid leukemia (AML) chemotherapy	167:207	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	11	10	theme	cell	1403:1406	arg1	lines					1408:1412	AML cell lines	1399:1412	AML cell lines	1399:1412	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	0	11	theme	myeloid	111:117	arg1	leukemia					119:126	acute myeloid leukemia	105:126	acute myeloid leukemia	105:126	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	13	12	theme	AML	1684:1686	arg1	cells					1688:1692	AML cells	1684:1692	AML cells	1684:1692	The observation suggested that high-mannose N-glycans and mannosyltransferase ALG3 affected drug-resistance in AML cells.					
29880818	6	13	theme	membrane	767:774	arg1	proteins					776:783	membrane proteins	767:783	membrane proteins	767:783	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	9	14	theme	AML	1117:1119	arg1	lines					1126:1130	AML cell lines	1117:1130	AML cell lines both in vitro and in vivo	1117:1156	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	14	15	theme	FTX/miR-342/ALG3	1695:1710	arg1	axis					1712:1715	FTX/miR-342/ALG3 axis	1695:1715	FTX/miR-342/ALG3 axis	1695:1715	FTX/miR-342/ALG3 axis could potentially be used for the targets to overcome therapeutic resistance in AML.					
29880818	0	16	theme	acute	105:109	arg1	leukemia					119:126	acute myeloid leukemia	105:126	acute myeloid leukemia	105:126	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	0	17	from	development	71:81	arg1	leukemia					119:126	acute myeloid leukemia	105:126	acute myeloid leukemia	105:126	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	1	18	from	problem	156:162	arg1	chemotherapy					196:207	acute myeloid leukemia (AML) chemotherapy	167:207	acute myeloid leukemia (AML) chemotherapy	167:207	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	10	19	theme	ALG3	1241:1244	arg1	expression					1246:1255	ALG3 expression	1241:1255	ALG3 expression	1241:1255	Mechanistically, miR-342 was found to be dysregulated and inversely correlated to ALG3 expression, targeting its 3'-UTR.					
29880818	4	20	theme	U937/ADR	546:553	arg1	cells					555:559	adriamycin (ADR)-resistant U937/ADR cells	519:559	adriamycin (ADR)-resistant U937/ADR cells	519:559	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	2	21	from	changes	219:225	arg1	N-glycans					239:247	specific N-glycans	230:247	specific N-glycans	230:247	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	1	22	theme	leukemia	181:188	arg1	chemotherapy					196:207	acute myeloid leukemia (AML) chemotherapy	167:207	acute myeloid leukemia (AML) chemotherapy	167:207	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	3	23	theme	coding	340:345	arg1	players					373:379	key players	369:379	key players in the development of AML resistance to chemotherapy	369:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	23	theme	coding	340:345	arg1	lncRNAs					353:359	lncRNAs	353:359	lncRNAs	353:359	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	23	theme	coding	340:345	arg1	MicroRNAs					308:316	MicroRNAs	308:316	MicroRNAs (miRNAs)	308:325	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	23	theme	coding	340:345	arg1	RNAs					347:350	long non coding RNAs	331:350	long non coding RNAs (lncRNAs)	331:360	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	12	24	dep	cell	1546:1549	arg1	apoptosis					1562:1570	apoptosis	1562:1570	apoptosis	1562:1570	Functionally, we found that FTX directly interacted with miR-342 to regulate ALG3 expression and function, including ADR-resistant cell growth and apoptosis.					
29880818	12	24	dep	cell	1546:1549	arg1	growth					1551:1556	growth	1551:1556	growth	1551:1556	Functionally, we found that FTX directly interacted with miR-342 to regulate ALG3 expression and function, including ADR-resistant cell growth and apoptosis.					
29880818	8	25	theme	enzyme	1004:1009	arg1	level					1011:1015	the enzyme level	1000:1015	the enzyme level of ALG family	1000:1029	Importantly, the alteration of high-mannose N-glycan was further confirmed by detecting the enzyme level of ALG family.					
29880818	6	26	theme	strong	749:754	arg1	binding					756:762	the strong binding	745:762	the strong binding of membrane proteins	745:783	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	2	27	theme	multidrug	280:288	arg1	MDR					302:304	MDR	302:304	MDR	302:304	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	2	27	theme	multidrug	280:288	arg1	resistance					290:299	leukemia multidrug resistance	271:299	leukemia multidrug resistance (MDR)	271:305	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	3	28	theme	resistance	407:416	arg1	development					388:398	the development	384:398	the development of AML resistance to chemotherapy	384:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	0	29	theme	mannosylation	9:21	arg1	profile					23:29	Aberrant mannosylation profile	0:29	Aberrant mannosylation profile	0:29	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	8	30	theme	ALG	1020:1022	arg1	family					1024:1029	ALG family	1020:1029	ALG family	1020:1029	Importantly, the alteration of high-mannose N-glycan was further confirmed by detecting the enzyme level of ALG family.					
29880818	8	31	theme	high-mannose	943:954	arg1	N-glycan					956:963	high-mannose N-glycan	943:963	high-mannose N-glycan	943:963	Importantly, the alteration of high-mannose N-glycan was further confirmed by detecting the enzyme level of ALG family.					
29880818	9	32	theme	altered	1036:1042	arg1	level					1044:1048	The altered level	1032:1048	The altered level of ALG3	1032:1056	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	0	33	theme	Aberrant	0:7	arg1	profile					23:29	Aberrant mannosylation profile	0:29	Aberrant mannosylation profile	0:29	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	12	34	theme	ADR-resistant	1532:1544	arg1	cell					1546:1549	ADR-resistant cell growth and apoptosis	1532:1570	ADR-resistant cell growth and apoptosis	1532:1570	Functionally, we found that FTX directly interacted with miR-342 to regulate ALG3 expression and function, including ADR-resistant cell growth and apoptosis.					
29880818	11	35	theme	miR-342	1314:1320	arg1	FTX					1287:1289	LncRNA FTX	1280:1289	LncRNA FTX	1280:1289	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	11	35	theme	miR-342	1314:1320	arg1	target					1304:1309	a direct target	1295:1309	a direct target of miR-342	1295:1320	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	11	36	theme	AML	1399:1401	arg1	lines					1408:1412	AML cell lines	1399:1412	AML cell lines	1399:1412	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	7	37	theme	flow	896:899	arg1	cytometry					901:909	flow cytometry	896:909	flow cytometry	896:909	These binding were also validated by flow cytometry.					
29880818	5	38	theme	high-mannose	650:661	arg1	N-glycans					663:671	high-mannose N-glycans	650:671	high-mannose N-glycans	650:671	The composition profiling of high-mannose N-glycans differed in U937/ADR and U937 cell lines.					
29880818	5	39	theme	cell	703:706	arg1	lines					708:712	U937 cell lines	698:712	U937 cell lines	698:712	The composition profiling of high-mannose N-glycans differed in U937/ADR and U937 cell lines.					
29880818	5	40	theme	N-glycans	663:671	arg1	composition					625:635	The composition profiling	621:645	The composition profiling of high-mannose N-glycans	621:671	The composition profiling of high-mannose N-glycans differed in U937/ADR and U937 cell lines.					
29880818	3	41	theme	non	336:338	arg1	players					373:379	key players	369:379	key players in the development of AML resistance to chemotherapy	369:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	41	theme	non	336:338	arg1	lncRNAs					353:359	lncRNAs	353:359	lncRNAs	353:359	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	41	theme	non	336:338	arg1	MicroRNAs					308:316	MicroRNAs	308:316	MicroRNAs (miRNAs)	308:325	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	41	theme	non	336:338	arg1	RNAs					347:350	long non coding RNAs	331:350	long non coding RNAs (lncRNAs)	331:360	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	9	42	theme	ALG3	1053:1056	arg1	level					1044:1048	The altered level	1032:1048	The altered level of ALG3	1032:1056	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	14	43	theme	therapeutic	1771:1781	arg1	resistance					1783:1792	therapeutic resistance	1771:1792	therapeutic resistance in AML	1771:1799	FTX/miR-342/ALG3 axis could potentially be used for the targets to overcome therapeutic resistance in AML.					
29880818	13	44	theme	high-mannose	1604:1615	arg1	N-glycans					1617:1625	high-mannose N-glycans	1604:1625	high-mannose N-glycans	1604:1625	The observation suggested that high-mannose N-glycans and mannosyltransferase ALG3 affected drug-resistance in AML cells.					
29880818	8	45	theme	family	1024:1029	arg1	level					1011:1015	the enzyme level	1000:1015	the enzyme level of ALG family	1000:1029	Importantly, the alteration of high-mannose N-glycan was further confirmed by detecting the enzyme level of ALG family.					
29880818	3	46	theme	AML	403:405	arg1	resistance					407:416	AML resistance	403:416	AML resistance to chemotherapy	403:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	4	47	theme	proteins	491:498	arg1	profiles					470:477	the N-glycan profiles	457:477	the N-glycan profiles of membrane proteins	457:498	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	6	48	theme	ConA	812:815	arg1	lectins					817:823	MAN-M and ConA lectins	802:823	MAN-M and ConA lectins	802:823	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	12	49	theme	ALG3	1492:1495	arg1	expression					1497:1506	ALG3 expression	1492:1506	ALG3 expression	1492:1506	Functionally, we found that FTX directly interacted with miR-342 to regulate ALG3 expression and function, including ADR-resistant cell growth and apoptosis.					
29880818	4	50	theme	mass	597:600	arg1	MS					616:617	MS	616:617	MS	616:617	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	4	50	theme	mass	597:600	arg1	spectrometry					602:613	mass spectrometry	597:613	mass spectrometry (MS)	597:618	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	3	51	theme	key	369:371	arg1	players					373:379	key players	369:379	key players in the development of AML resistance to chemotherapy	369:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	51	theme	key	369:371	arg1	RNAs					347:350	long non coding RNAs	331:350	long non coding RNAs (lncRNAs)	331:360	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	51	theme	key	369:371	arg1	MicroRNAs					308:316	MicroRNAs	308:316	MicroRNAs (miRNAs)	308:325	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	4	52	theme	membrane	482:489	arg1	proteins					491:498	membrane proteins	482:498	membrane proteins	482:498	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	11	53	theme	ALG3	1347:1350	arg1	expression					1352:1361	ALG3 expression	1347:1361	ALG3 expression	1347:1361	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	2	54	theme	specific	230:237	arg1	N-glycans					239:247	specific N-glycans	230:247	specific N-glycans	230:247	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	6	55	theme	Lectin	715:720	arg1	microarray					722:731	Lectin microarray	715:731	Lectin microarray	715:731	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	9	56	theme	cell	1121:1124	arg1	lines					1126:1130	AML cell lines	1117:1130	AML cell lines both in vitro and in vivo	1117:1156	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	6	57	theme	MAN-M	802:806	arg1	lectins					817:823	MAN-M and ConA lectins	802:823	MAN-M and ConA lectins	802:823	Lectin microarray showed that the strong binding of membrane proteins was observed for MAN-M and ConA lectins, which were specific for mannose.					
29880818	13	58	theme	mannosyltransferase	1631:1649	arg1	ALG3					1651:1654	mannosyltransferase ALG3	1631:1654	mannosyltransferase ALG3	1631:1654	The observation suggested that high-mannose N-glycans and mannosyltransferase ALG3 affected drug-resistance in AML cells.					
29880818	5	59	theme	profiling	637:645	arg1	composition					625:635	The composition profiling	621:645	The composition profiling of high-mannose N-glycans	621:671	The composition profiling of high-mannose N-glycans differed in U937/ADR and U937 cell lines.					
29880818	2	60	theme	Aberrant	210:217	arg1	changes					219:225	Aberrant changes	210:225	Aberrant changes in specific N-glycans	210:247	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	2	61	theme	leukemia	271:278	arg1	MDR					302:304	MDR	302:304	MDR	302:304	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	2	61	theme	leukemia	271:278	arg1	resistance					290:299	leukemia multidrug resistance	271:299	leukemia multidrug resistance (MDR)	271:305	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	9	62	theme	lines	1126:1130	arg1	phenotype					1104:1112	the drug-resistant phenotype	1085:1112	the drug-resistant phenotype of AML cell lines both in vitro and in vivo	1085:1156	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	14	63	used	used	1738:1741	arg2	axis					1712:1715	FTX/miR-342/ALG3 axis	1695:1715	FTX/miR-342/ALG3 axis	1695:1715	FTX/miR-342/ALG3 axis could potentially be used for the targets to overcome therapeutic resistance in AML.					
29880818	4	64	theme	sensitive	565:573	arg1	cells					585:589	sensitive line U937 cells	565:589	sensitive line U937 cells	565:589	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	9	65	theme	drug-resistant	1089:1102	arg1	phenotype					1104:1112	the drug-resistant phenotype	1085:1112	the drug-resistant phenotype of AML cell lines both in vitro and in vivo	1085:1156	The altered level of ALG3 was found corresponding to the drug-resistant phenotype of AML cell lines both in vitro and in vivo.					
29880818	1	66	theme	major	150:154	arg1	Drug-resistance					129:143	Drug-resistance	129:143	Drug-resistance	129:143	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	1	66	theme	major	150:154	arg1	problem					156:162	a major problem	148:162	a major problem in acute myeloid leukemia (AML) chemotherapy	148:207	Drug-resistance is a major problem in acute myeloid leukemia (AML) chemotherapy.					
29880818	11	67	from	miR-342	1388:1394	arg1	lines					1408:1412	AML cell lines	1399:1412	AML cell lines	1399:1412	LncRNA FTX was a direct target of miR-342 and positively modulated ALG3 expression by competitively binding miR-342 in AML cell lines.					
29880818	0	68	theme	drug	86:89	arg1	resistance					91:100	drug resistance	86:100	drug resistance	86:100	Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.					
29880818	5	69	theme	U937	698:701	arg1	lines					708:712	U937 cell lines	698:712	U937 cell lines	698:712	The composition profiling of high-mannose N-glycans differed in U937/ADR and U937 cell lines.					
29880818	4	70	theme	U937	580:583	arg1	cells					585:589	sensitive line U937 cells	565:589	sensitive line U937 cells	565:589	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	3	71	theme	long	331:334	arg1	players					373:379	key players	369:379	key players in the development of AML resistance to chemotherapy	369:432	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	71	theme	long	331:334	arg1	lncRNAs					353:359	lncRNAs	353:359	lncRNAs	353:359	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	71	theme	long	331:334	arg1	MicroRNAs					308:316	MicroRNAs	308:316	MicroRNAs (miRNAs)	308:325	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	3	71	theme	long	331:334	arg1	RNAs					347:350	long non coding RNAs	331:350	long non coding RNAs (lncRNAs)	331:360	MicroRNAs (miRNAs) and long non coding RNAs (lncRNAs) act as key players in the development of AML resistance to chemotherapy.					
29880818	4	72	theme	N-glycan	461:468	arg1	profiles					470:477	the N-glycan profiles	457:477	the N-glycan profiles of membrane proteins	457:498	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	4	73	theme	line	575:578	arg1	cells					585:589	sensitive line U937 cells	565:589	sensitive line U937 cells	565:589	In the present study, the N-glycan profiles of membrane proteins were analyzed from adriamycin (ADR)-resistant U937/ADR cells and sensitive line U937 cells using mass spectrometry (MS).					
29880818	14	74	from	resistance	1783:1792	arg1	AML					1797:1799	AML	1797:1799	AML	1797:1799	FTX/miR-342/ALG3 axis could potentially be used for the targets to overcome therapeutic resistance in AML.					
29880818	2	75	located	observed	259:266	arg2	changes					219:225	Aberrant changes	210:225	Aberrant changes in specific N-glycans	210:247	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	2	75	located	observed	259:266	arg1	MDR					302:304	MDR	302:304	MDR	302:304	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
29880818	2	75	located	observed	259:266	arg1	resistance					290:299	leukemia multidrug resistance	271:299	leukemia multidrug resistance (MDR)	271:305	Aberrant changes in specific N-glycans have been observed in leukemia multidrug resistance (MDR).					
30708016	0	0	theme	subsp	76:80	arg1	roots					40:44	the roots	36:44	the roots of Eremurus spectabilis M. B. subsp	36:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	3	1	theme	Specific	317:324	arg1	rotation					334:341	Specific optical rotation	317:341	Specific optical rotation	317:341	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	0	2	theme	B.	73:74	arg1	subsp					76:80	Eremurus spectabilis M. B. subsp	49:80	Eremurus spectabilis M. B. subsp	49:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	6	3	theme	ESP	774:776	arg1	unit					766:769	The repeating unit	752:769	The repeating unit of ESP	752:776	The repeating unit of ESP was determined as following.					
30708016	6	3	theme	ESP	774:776	arg1	following					796:804	following	796:804	following	796:804	The repeating unit of ESP was determined as following.					
30708016	3	4	theme	total	363:367	arg1	content					382:388	total carbohydrate content	363:388	total carbohydrate content	363:388	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	2	5	theme	heteropolysaccharide	148:167	arg1	ESP					169:171	A heteropolysaccharide ESP	146:171	A heteropolysaccharide ESP	146:171	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	5	6	theme	partial	664:670	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	6	theme	partial	664:670	arg1	hydrolysis					677:686	partial acid hydrolysis	664:686	partial acid hydrolysis	664:686	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	3	7	theme	molecular	344:352	arg1	mass					354:357	molecular mass	344:357	molecular mass	344:357	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	5	8	theme	chemical	613:620	arg1	techniques					639:648	various chemical and instrumental techniques	605:648	various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR)	605:749	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	9	theme	various	605:611	arg1	techniques					639:648	various chemical and instrumental techniques	605:648	various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR)	605:749	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	10	theme	1D/2D	740:744	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	10	theme	1D/2D	740:744	arg1	NMR					746:748	1D/2D NMR	740:748	1D/2D NMR	740:748	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	0	11	from	roots	40:44	arg1	polysaccharide					16:29	A water-soluble polysaccharide	0:29	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp	0:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	5	12	theme	Structural	558:567	arg1	characterization					569:584	Structural characterization	558:584	Structural characterization of ESP	558:591	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	0	13	theme	water-soluble	2:14	arg1	polysaccharide					16:29	A water-soluble polysaccharide	0:29	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp	0:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	3	14	theme	optical	326:332	arg1	rotation					334:341	Specific optical rotation	317:341	Specific optical rotation	317:341	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	5	15	theme	instrumental	626:637	arg1	techniques					639:648	various chemical and instrumental techniques	605:648	various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR)	605:749	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	3	16	theme	ESP	393:395	arg1	rotation					334:341	Specific optical rotation	317:341	Specific optical rotation	317:341	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	3	16	theme	ESP	393:395	arg1	mass					354:357	molecular mass	344:357	molecular mass	344:357	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	3	16	theme	ESP	393:395	arg1	content					382:388	total carbohydrate content	363:388	total carbohydrate content	363:388	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	3	17	theme	97.2	423:426	arg1	%					427:427	-36.3°, 43.8 kDa and 97.2%	402:427	%	427:427	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	3	18	theme	carbohydrate	369:380	arg1	content					382:388	total carbohydrate content	363:388	total carbohydrate content	363:388	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	5	19	theme	ESP	589:591	arg1	characterization					569:584	Structural characterization	558:584	Structural characterization of ESP	558:591	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	2	20	theme	Sephadex	301:308	arg1	G-100					310:314	Sephadex G-100	301:314	Sephadex G-100	301:314	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	4	21	theme	1.0:2.9	549:555	arg1	ratio					540:544	a molar ratio	532:544	a molar ratio of 1.0:2.9	532:555	Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9.					
30708016	5	22	theme	periodate	708:716	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	22	theme	periodate	708:716	arg1	oxidation					718:726	periodate oxidation	708:726	periodate oxidation	708:726	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	NMR					746:748	1D/2D NMR	740:748	1D/2D NMR	740:748	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	oxidation					718:726	periodate oxidation	708:726	periodate oxidation	708:726	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	hydrolysis					677:686	partial acid hydrolysis	664:686	partial acid hydrolysis	664:686	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	GLC-MS					729:734	GLC-MS	729:734	GLC-MS	729:734	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	23	dep	techniques	639:648	arg1	degradation					695:705	Smith degradation	689:705	Smith degradation	689:705	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	4	24	theme	composition	459:469	arg1	analysis					471:478	Monosaccharide composition analysis	444:478	Monosaccharide composition analysis	444:478	Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9.					
30708016	2	25	theme	roots	218:222	arg1	extract					201:207	a water extract	193:207	a water extract of dried roots of Eremurus spectabilis	193:246	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	0	26	theme	Eremurus	49:56	arg1	subsp					76:80	Eremurus spectabilis M. B. subsp	49:80	Eremurus spectabilis M. B. subsp	49:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	5	27	theme	acid	672:675	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	27	theme	acid	672:675	arg1	hydrolysis					677:686	partial acid hydrolysis	664:686	partial acid hydrolysis	664:686	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	4	28	theme	Monosaccharide	444:457	arg1	analysis					471:478	Monosaccharide composition analysis	444:478	Monosaccharide composition analysis	444:478	Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9.					
30708016	2	29	theme	dried	212:216	arg1	roots					218:222	dried roots	212:222	dried roots of Eremurus spectabilis	212:246	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	3	30	theme	-36.3°	402:407	arg1	%					427:427	-36.3°, 43.8 kDa and 97.2%	402:427	%	427:427	Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively.					
30708016	4	31	theme	molar	534:538	arg1	ratio					540:544	a molar ratio	532:544	a molar ratio of 1.0:2.9	532:555	Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9.					
30708016	6	32	theme	repeating	756:764	arg1	unit					766:769	The repeating unit	752:769	The repeating unit of ESP	752:776	The repeating unit of ESP was determined as following.					
30708016	6	32	theme	repeating	756:764	arg1	following					796:804	following	796:804	following	796:804	The repeating unit of ESP was determined as following.					
30708016	2	33	theme	water	195:199	arg1	extract					201:207	a water extract	193:207	a water extract of dried roots of Eremurus spectabilis	193:246	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	2	34	theme	column	251:256	arg1	chromatographies					258:273	column chromatographies	251:273	column chromatographies on DEAE-cellulose A52 and Sephadex G-100	251:314	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	2	35	from	chromatographies	258:273	arg1	DEAE-cellulose					278:291	DEAE-cellulose A52	278:295	DEAE-cellulose A52	278:295	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	2	35	from	chromatographies	258:273	arg1	G-100					310:314	Sephadex G-100	301:314	Sephadex G-100	301:314	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	5	36	theme	Smith	689:693	arg1	methylation					651:661	methylation	651:661	methylation	651:661	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	5	36	theme	Smith	689:693	arg1	degradation					695:705	Smith degradation	689:705	Smith degradation	689:705	Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR).					
30708016	1	37	theme	structural	125:134	arg1	features					136:143	structural features	125:143	structural features	125:143	spectabilis: Extraction, purification and structural features.					
30708016	0	38	theme	M.	70:71	arg1	subsp					76:80	Eremurus spectabilis M. B. subsp	49:80	Eremurus spectabilis M. B. subsp	49:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	2	39	theme	spectabilis	236:246	arg1	roots					218:222	dried roots	212:222	dried roots of Eremurus spectabilis	212:246	A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100.					
30708016	0	40	theme	spectabilis	58:68	arg1	subsp					76:80	Eremurus spectabilis M. B. subsp	49:80	Eremurus spectabilis M. B. subsp	49:80	A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp.					
30708016	1	41	dep	Extraction	96:105	arg1	spectabilis					83:93	spectabilis	83:93	spectabilis	83:93	spectabilis: Extraction, purification and structural features.					
31125199	7	0	theme	β-	1006:1007	arg1	MCs					1029:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	1	1	theme	wall	209:212	arg1	components					158:167	the antigenic components	144:167	the antigenic components of the bacterial as well as fungal cell wall	144:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	7	2	theme	grafted	1021:1027	arg1	MCs					1029:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	8	3	theme	MCs	1276:1278	arg1	phagocytosis					1231:1242	the phagocytosis	1227:1242	the phagocytosis of these β-(1,3)-glucan grafted MCs	1227:1278	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	0	4	theme	Cell	92:95	arg1	Polysaccharides					102:116	Microbial Cell Wall Polysaccharides	82:116	Microbial Cell Wall Polysaccharides	82:116	β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.					
31125199	9	5	theme	polysaccharides	1507:1521	arg1	effect					1483:1488	immunomodulatory effect	1466:1488	immunomodulatory effect of the microbial polysaccharides	1466:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	9	5	theme	polysaccharides	1507:1521	arg1	mechanism					1436:1444	the mechanism	1432:1444	the mechanism of phagocytosis	1432:1460	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	7	6	theme	human	1054:1058	arg1	macrophages					1077:1087	human monocyte-derived macrophages	1054:1087	human monocyte-derived macrophages	1054:1087	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	8	7	theme	-glucan	1260:1266	arg1	MCs					1276:1278	these β-(1,3)-glucan grafted MCs	1247:1278	these β-(1,3)-glucan grafted MCs	1247:1278	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	4	8	theme	formation	608:616	arg1	kinetics					591:598	the kinetics	587:598	the kinetics of film formation	587:616	Planar films were first designed to optimize monomer compositions and to examine the kinetics of film formation.					
31125199	0	9	theme	Microbial	82:90	arg1	Polysaccharides					102:116	Microbial Cell Wall Polysaccharides	82:116	Microbial Cell Wall Polysaccharides	82:116	β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.					
31125199	6	10	theme	1,3	880:882	arg1	-glucan					884:890	a linear β-(1,3)-glucan	868:890	a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus	868:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	10	theme	1,3	880:882	arg1	Curdlan					859:865	Curdlan	859:865	Curdlan	859:865	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	7	11	theme	monocyte-derived	1060:1075	arg1	macrophages					1077:1087	human monocyte-derived macrophages	1054:1087	human monocyte-derived macrophages	1054:1087	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	0	12	theme	Polysaccharides	102:116	arg1	Effect					72:77	the Immunomodulatory Effect	51:77	the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides	51:116	β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.					
31125199	7	13	link	monocyte-derived	1060:1075	arg1	macrophages					1077:1087	human monocyte-derived macrophages	1054:1087	human monocyte-derived macrophages	1054:1087	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	3	14	theme	aqueous	476:482	arg1	phases					484:489	organic and aqueous phases	464:489	phases	484:489	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	3	15	theme	diacyl	376:381	arg1	chloride					383:390	diacyl chloride	376:390	diacyl chloride (sebacoyl chloride and terephtaloyl chloride)	376:436	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	0	16	theme	Wall	97:100	arg1	Polysaccharides					102:116	Microbial Cell Wall Polysaccharides	82:116	Microbial Cell Wall Polysaccharides	82:116	β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.					
31125199	6	17	theme	β-	877:878	arg1	-glucan					884:890	a linear β-(1,3)-glucan	868:890	a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus	868:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	17	theme	β-	877:878	arg1	Curdlan					859:865	Curdlan	859:865	Curdlan	859:865	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	18	theme	cell	964:967	arg1	wall					969:972	the cell wall	960:972	the cell wall of Aspergillus fumigatus	960:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	8	19	theme	grafted	1268:1274	arg1	MCs					1276:1278	these β-(1,3)-glucan grafted MCs	1247:1278	these β-(1,3)-glucan grafted MCs	1247:1278	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	4	20	theme	film	603:606	arg1	formation					608:616	film formation	603:616	film formation	603:616	Planar films were first designed to optimize monomer compositions and to examine the kinetics of film formation.					
31125199	5	21	theme	aqueous	799:805	arg1	phase					807:811	the aqueous phase	795:811	the aqueous phase	795:811	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	6	22	theme	linear	870:875	arg1	-glucan					884:890	a linear β-(1,3)-glucan	868:890	a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus	868:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	22	theme	linear	870:875	arg1	Curdlan					859:865	Curdlan	859:865	Curdlan	859:865	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	5	23	theme	optimized	736:744	arg1	compositions					754:765	optimized monomer compositions	736:765	optimized monomer compositions	736:765	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	9	24	theme	mechanism	1436:1444	arg1	study					1423:1427	the study	1419:1427	the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides	1419:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	6	25	from	species	911:917	arg1	-glucan					884:890	a linear β-(1,3)-glucan	868:890	a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus	868:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	25	from	species	911:917	arg1	Curdlan					859:865	Curdlan	859:865	Curdlan	859:865	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	1	26	theme	antigenic	148:156	arg1	components					158:167	the antigenic components	144:167	the antigenic components of the bacterial as well as fungal cell wall	144:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	4	27	theme	Planar	506:511	arg1	films					513:517	Planar films	506:517	Planar films	506:517	Planar films were first designed to optimize monomer compositions and to examine the kinetics of film formation.					
31125199	2	28	theme	β-	260:261	arg1	-glucan					267:273	β-(1,3)-glucan	260:273	β-(1,3)-glucan	260:273	We designed microcapsules (MCs) ligated with β-(1,3)-glucan, to study its immunomodulatory effect.					
31125199	1	29	theme	components	158:167	arg1	components					158:167	the antigenic components	144:167	the antigenic components of the bacterial as well as fungal cell wall	144:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	1	29	theme	components	158:167	arg1	one					137:139	one	137:139	one	137:139	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	7	30	theme	-glucan	1013:1019	arg1	MCs					1029:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs	1000:1031	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	8	31	theme	receptors	1317:1325	arg1	involvement					1296:1306	the involvement	1292:1306	the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan	1292:1373	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	8	32	theme	β-	1163:1164	arg1	dectin-1					1151:1158	dectin-1	1151:1158	dectin-1	1151:1158	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	8	32	theme	β-	1163:1164	arg1	-glucan					1170:1176	a β-(1,3)-glucan	1161:1176	a β-(1,3)-glucan recognizing receptor	1161:1197	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	8	32	theme	β-	1163:1164	arg1	1,3					1166:1168	1,3	1166:1168	1,3	1166:1168	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	9	33	theme	immunomodulatory	1466:1481	arg1	effect					1483:1488	immunomodulatory effect	1466:1488	immunomodulatory effect of the microbial polysaccharides	1466:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	8	34	theme	dectin-1	1151:1158	arg1	blocking					1139:1146	the blocking	1135:1146	the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor,	1135:1198	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	6	35	theme	1,3	934:936	arg1	-glucan					938:944	branched β-(1,3)-glucan	922:944	branched β-(1,3)-glucan	922:944	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	36	attach	isolated	946:953	arg2	species					911:917	Agrobacterium species	897:917	Agrobacterium species	897:917	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	36	attach	isolated	946:953	arg1	wall					969:972	the cell wall	960:972	the cell wall of Aspergillus fumigatus	960:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	8	37	theme	β-	1360:1361	arg1	-glucan					1367:1373	β-(1,3)-glucan	1360:1373	β-(1,3)-glucan	1360:1373	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	2	38	theme	immunomodulatory	289:304	arg1	effect					306:311	its immunomodulatory effect	285:311	its immunomodulatory effect	285:311	We designed microcapsules (MCs) ligated with β-(1,3)-glucan, to study its immunomodulatory effect.					
31125199	7	39	theme	cytokine	1105:1112	arg1	secretion					1114:1122	cytokine secretion	1105:1122	cytokine secretion	1105:1122	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	9	40	theme	grafted	1385:1391	arg1	MCs					1393:1395	grafted MCs	1385:1395	grafted MCs	1385:1395	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	9	40	theme	grafted	1385:1391	arg1	tool					1410:1413	a useful tool	1401:1413	a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides	1401:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	6	41	from	-glucan	938:944	arg1	-glucan					884:890	a linear β-(1,3)-glucan	868:890	a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus	868:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	6	41	from	-glucan	938:944	arg1	Curdlan					859:865	Curdlan	859:865	Curdlan	859:865	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	8	42	theme	other	1311:1315	arg1	receptors					1317:1325	other receptors	1311:1325	other receptors	1311:1325	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	1	43	theme	bacterial	176:184	arg1	wall					209:212	the bacterial as well as fungal cell wall	172:212	the bacterial as well as fungal cell wall	172:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	3	44	theme	interfacial	339:349	arg1	polycondensation					351:366	interfacial polycondensation	339:366	interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases	339:489	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	6	45	theme	β-	931:932	arg1	-glucan					938:944	branched β-(1,3)-glucan	922:944	branched β-(1,3)-glucan	922:944	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	5	46	theme	emulsification-polycondensation	688:718	arg1	reactions					720:728	controlled emulsification-polycondensation reactions	677:728	controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase	677:811	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	8	47	dep	recognition	1334:1344	arg1	the					1330:1332	the	1330:1332	the	1330:1332	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	3	48	theme	sebacoyl	393:400	arg1	chloride					402:409	sebacoyl chloride	393:409	sebacoyl chloride	393:409	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	5	49	theme	controlled	677:686	arg1	reactions					720:728	controlled emulsification-polycondensation reactions	677:728	controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase	677:811	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	5	50	dep	reactions	720:728	arg1	adding					771:776	adding	771:776	adding fluorescein into the aqueous phase	771:811	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	5	50	dep	reactions	720:728	arg1	using					730:734	using	730:734	using optimized monomer compositions	730:765	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	8	51	dep	β-	1253:1254	arg1	1,3					1256:1258	1,3	1256:1258	1,3	1256:1258	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	7	52	dep	β-	1006:1007	arg1	1,3					1009:1011	1,3	1009:1011	1,3	1009:1011	These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion.					
31125199	5	53	theme	aqueous	628:634	arg1	core					648:651	aqueous fluorescent core	628:651	aqueous fluorescent core	628:651	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	8	54	theme	-glucan	1367:1373	arg1	recognition					1334:1344	recognition	1334:1344	recognition	1334:1344	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	8	54	theme	-glucan	1367:1373	arg1	uptake					1350:1355	uptake	1350:1355	uptake	1350:1355	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	3	55	dep	chloride	383:390	arg1	chloride					402:409	sebacoyl chloride	393:409	sebacoyl chloride	393:409	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	3	55	dep	chloride	383:390	arg1	chloride					428:435	terephtaloyl chloride	415:435	terephtaloyl chloride	415:435	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	1	56	theme	β-	119:120	arg1	-Glucan					126:132	β-(1,3)-Glucan	119:132	β-(1,3)-Glucan	119:132	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	5	57	theme	fluorescent	636:646	arg1	core					648:651	aqueous fluorescent core	628:651	aqueous fluorescent core	628:651	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	9	58	theme	microbial	1497:1505	arg1	polysaccharides					1507:1521	the microbial polysaccharides	1493:1521	the microbial polysaccharides	1493:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	9	59	theme	effect	1483:1488	arg1	study					1423:1427	the study	1419:1427	the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides	1419:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	3	60	theme	terephtaloyl	415:426	arg1	chloride					428:435	terephtaloyl chloride	415:435	terephtaloyl chloride	415:435	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	9	61	theme	phagocytosis	1449:1460	arg1	effect					1483:1488	immunomodulatory effect	1466:1488	immunomodulatory effect of the microbial polysaccharides	1466:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	9	61	theme	phagocytosis	1449:1460	arg1	mechanism					1436:1444	the mechanism	1432:1444	the mechanism of phagocytosis	1432:1460	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	6	62	theme	fumigatus	989:997	arg1	wall					969:972	the cell wall	960:972	the cell wall of Aspergillus fumigatus	960:997	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	8	63	theme	β-	1253:1254	arg1	MCs					1276:1278	these β-(1,3)-glucan grafted MCs	1247:1278	these β-(1,3)-glucan grafted MCs	1247:1278	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	3	64	from	polycondensation	351:366	arg1	phases					484:489	organic and aqueous phases	464:489	phases	484:489	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	9	65	theme	useful	1403:1408	arg1	MCs					1393:1395	grafted MCs	1385:1395	grafted MCs	1385:1395	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	9	65	theme	useful	1403:1408	arg1	tool					1410:1413	a useful tool	1401:1413	a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides	1401:1521	Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.					
31125199	4	66	theme	monomer	551:557	arg1	compositions					559:570	monomer compositions	551:570	monomer compositions	551:570	Planar films were first designed to optimize monomer compositions and to examine the kinetics of film formation.					
31125199	0	67	theme	Immunomodulatory	55:70	arg1	Effect					72:77	the Immunomodulatory Effect	51:77	the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides	51:116	β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.					
31125199	6	68	theme	selected	818:825	arg1	MC-formulation					827:840	The selected MC-formulation	814:840	The selected MC-formulation	814:840	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	8	69	from	involvement	1296:1306	arg1	recognition					1334:1344	recognition	1334:1344	recognition	1334:1344	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	8	69	from	involvement	1296:1306	arg1	uptake					1350:1355	uptake	1350:1355	uptake	1350:1355	Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan.					
31125199	3	70	theme	organic	464:470	arg1	phases					484:489	organic and aqueous phases	464:489	phases	484:489	The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively.					
31125199	1	71	theme	fungal	197:202	arg1	wall					209:212	the bacterial as well as fungal cell wall	172:212	the bacterial as well as fungal cell wall	172:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
31125199	5	72	theme	monomer	746:752	arg1	compositions					754:765	optimized monomer compositions	736:765	optimized monomer compositions	736:765	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	6	73	theme	Agrobacterium	897:909	arg1	species					911:917	Agrobacterium species	897:917	Agrobacterium species	897:917	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	5	74	with	MCs	619:621	arg1	core					648:651	aqueous fluorescent core	628:651	aqueous fluorescent core	628:651	MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase.					
31125199	6	75	theme	branched	922:929	arg1	-glucan					938:944	branched β-(1,3)-glucan	922:944	branched β-(1,3)-glucan	922:944	The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus.					
31125199	1	76	theme	cell	204:207	arg1	wall					209:212	the bacterial as well as fungal cell wall	172:212	the bacterial as well as fungal cell wall	172:212	β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall.					
30782133	6	0	theme	polysaccharide	818:831	arg1	mobilities					833:842	polysaccharide mobilities	818:842	polysaccharide mobilities	818:842	Additionally, solid-state NMR spectroscopy was used to examine changes in polysaccharide mobilities during development.					
30782133	7	1	theme	collenchyma	886:896	arg1	walls					898:902	the collenchyma walls	882:902	the collenchyma walls	882:902	RESULTS We showed the collenchyma walls are deposited only during cell expansion, i.e. they are primary walls.					
30782133	8	2	theme	cell-wall	982:990	arg1	development					992:1002	cell-wall development	982:1002	cell-wall development	982:1002	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	8	3	theme	methyl	1196:1201	arg1	esterification					1203:1216	their methyl esterification	1190:1216	their methyl esterification	1190:1216	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	12	4	theme	middle	1758:1763	arg1	junctions					1773:1781	middle lamella junctions	1758:1781	middle lamella junctions	1758:1781	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	11	5	theme	lamella	1589:1595	arg1	junctions					1597:1605	middle lamella junctions	1582:1605	middle lamella junctions	1582:1605	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	16	6	dep	CONCLUSIONS	2247:2257	arg1	showed					2271:2276	showed	2271:2276	showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes	2271:2401	CONCLUSIONS Our studies showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes.					
30782133	2	7	dep	thick	262:266	arg1	non-lignified					269:281	non-lignified	269:281	non-lignified	269:281	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	5	8	theme	chemical	614:621	arg1	analysis					623:630	chemical analysis	614:630	chemical analysis	614:630	We used chemical analysis and immunomicroscopy to determine changes in the polysaccharide compositions of these walls during development.					
30782133	17	9	dep	expansion	2521:2529	arg1	ceases					2531:2536	ceases	2531:2536	ceases	2531:2536	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	1	10	theme	growing	200:206	arg1	organs					208:213	growing organs	200:213	growing organs	200:213	BACKGROUND Collenchyma cells occur widely in eudicotyledons and provide mechanical support for growing organs.					
30782133	12	11	theme	1 → 4	1622:1626	arg1	-β-D-galactans					1628:1641	the (1 → 4)-β-D-galactans	1617:1641	the (1 → 4)-β-D-galactans	1617:1641	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	11	12	theme	middle	1562:1567	arg1	lamellae					1569:1576	the middle lamellae	1558:1576	the middle lamellae	1558:1576	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	15	13	theme	Solid-state	2112:2122	arg1	spectroscopy					2128:2139	Solid-state NMR spectroscopy	2112:2139	Solid-state NMR spectroscopy	2112:2139	Solid-state NMR spectroscopy showed that particularly cellulose, but also homogalacturonans, decreased in mobility during development.					
30782133	17	14	theme	cell	2490:2493	arg1	walls					2495:2499	the cell walls	2486:2499	the cell walls becoming stiffer as expansion ceases	2486:2536	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	9	15	from	decrease	1236:1243	arg1	proportions					1252:1262	the proportions	1248:1262	the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1248:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	14	16	theme	mature	2098:2103	arg1	cells					2105:2109	mature cells	2098:2109	mature cells	2098:2109	The location of the heteroxylans also changed, occurring mostly in the outer walls in young cells, but were more widely distributed in mature cells.					
30782133	17	17	from	homogalacturonans	2447:2463	arg1	mobilities					2419:2428	the mobilities	2415:2428	the mobilities of cellulose	2415:2441	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	11	18	from	junctions	1597:1605	arg1	abundant					1546:1553	abundant	1546:1553	abundant	1546:1553	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	11	19	dep	showed	1473:1478	arg1	occurred					1502:1509	occurred	1502:1509	occurred throughout the walls	1502:1530	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	11	19	dep	showed	1473:1478	arg1	abundant					1546:1553	abundant	1546:1553	abundant	1546:1553	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	16	20	theme	celery	2283:2288	arg1	walls					2307:2311	celery collenchyma cell walls	2283:2311	celery collenchyma cell walls	2283:2311	CONCLUSIONS Our studies showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes.					
30782133	13	21	theme	lamellae	1874:1881	arg1	junctions					1902:1910	the middle lamellae and middle lamella junctions	1863:1910	the middle lamellae and middle lamella junctions early on	1863:1919	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	11	22	from	lamellae	1569:1576	arg1	abundant					1546:1553	abundant	1546:1553	abundant	1546:1553	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	12	23	theme	lamella	1765:1771	arg1	junctions					1773:1781	middle lamella junctions	1758:1781	middle lamella junctions	1758:1781	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	13	24	theme	middle	1887:1892	arg1	lamella					1894:1900	middle lamella	1887:1900	middle lamella	1887:1900	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	9	25	theme	-β-D-galactan	1303:1315	arg1	chains					1322:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains	1267:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1267:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	7	26	theme	primary	960:966	arg1	they					951:954	they	951:954	they	951:954	RESULTS We showed the collenchyma walls are deposited only during cell expansion, i.e. they are primary walls.					
30782133	7	26	theme	primary	960:966	arg1	walls					968:972	primary walls	960:972	primary walls	960:972	RESULTS We showed the collenchyma walls are deposited only during cell expansion, i.e. they are primary walls.					
30782133	13	27	theme	early	1912:1916	arg1	junctions					1902:1910	the middle lamellae and middle lamella junctions	1863:1910	the middle lamellae and middle lamella junctions early on	1863:1919	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	9	28	theme	chains	1322:1327	arg1	proportions					1252:1262	the proportions	1248:1262	the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1248:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	11	29	from	abundant	1546:1553	arg1	lamellae					1569:1576	the middle lamellae	1558:1576	the middle lamellae	1558:1576	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	11	29	from	abundant	1546:1553	arg1	junctions					1597:1605	middle lamella junctions	1582:1605	middle lamella junctions	1582:1605	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	0	30	theme	Developmental	0:12	arg1	changes					14:20	Developmental changes	0:20	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.	0:103	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	9	31	theme	rhamnogalacturonan	1332:1349	arg1	chains					1322:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains	1267:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1267:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	14	32	theme	young	2049:2053	arg1	cells					2055:2059	young cells	2049:2059	young cells	2049:2059	The location of the heteroxylans also changed, occurring mostly in the outer walls in young cells, but were more widely distributed in mature cells.					
30782133	13	33	theme	xyloglucans	1824:1834	arg1	location					1808:1815	the location	1804:1815	the location of the xyloglucans	1804:1834	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	8	34	theme	overall	1105:1111	arg1	changes					1113:1119	no overall changes	1102:1119	no overall changes in the proportions of homogalacturonans	1102:1159	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	14	35	from	walls	2040:2044	arg1	cells					2055:2059	young cells	2049:2059	young cells	2049:2059	The location of the heteroxylans also changed, occurring mostly in the outer walls in young cells, but were more widely distributed in mature cells.					
30782133	1	36	theme	BACKGROUND	105:114	arg1	cells					128:132	BACKGROUND Collenchyma cells	105:132	BACKGROUND Collenchyma cells	105:132	BACKGROUND Collenchyma cells occur widely in eudicotyledons and provide mechanical support for growing organs.					
30782133	3	37	theme	previous	416:423	arg1	study					425:429	A previous study	414:429	A previous study	414:429	A previous study suggested that at least some of the collenchyma cell wall in celery is laid down after expansion has stopped and is thus secondary.					
30782133	0	38	theme	cell-wall	37:45	arg1	polysaccharides					47:61	collenchyma cell-wall polysaccharides	25:61	collenchyma cell-wall polysaccharides	25:61	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	16	39	theme	dynamic	2387:2393	arg1	changes					2395:2401	dynamic changes	2387:2401	dynamic changes	2387:2401	CONCLUSIONS Our studies showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes.					
30782133	3	40	theme	cell	479:482	arg1	wall					484:487	the collenchyma cell wall	463:487	the collenchyma cell wall in celery	463:497	A previous study suggested that at least some of the collenchyma cell wall in celery is laid down after expansion has stopped and is thus secondary.					
30782133	8	41	theme	homogalacturonans	1143:1159	arg1	proportions					1128:1138	the proportions	1124:1138	the proportions of homogalacturonans	1124:1159	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	7	42	dep	RESULTS	864:870	arg1	showed					875:880	showed	875:880	showed the collenchyma walls	875:902	RESULTS We showed the collenchyma walls are deposited only during cell expansion, i.e. they are primary walls.					
30782133	12	43	dep	walls	1676:1680	arg1	the					1660:1662	the	1660:1662	the	1660:1662	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	12	43	dep	walls	1676:1680	arg1	rest					1664:1667	rest	1664:1667	rest	1664:1667	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	5	44	used	used	609:612	arg2	We					606:607	We	606:607	We	606:607	We used chemical analysis and immunomicroscopy to determine changes in the polysaccharide compositions of these walls during development.					
30782133	2	45	contain	have	257:260	arg1	cells					233:237	the cells	229:237	the cells	229:237	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	2	45	contain	have	257:260	arg2	walls					283:287	thick, non-lignified walls	262:287	thick, non-lignified walls	262:287	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	6	46	theme	solid-state	758:768	arg1	spectroscopy					774:785	solid-state NMR spectroscopy	758:785	solid-state NMR spectroscopy	758:785	Additionally, solid-state NMR spectroscopy was used to examine changes in polysaccharide mobilities during development.					
30782133	8	47	theme	analytical	1005:1014	arg1	studies					1037:1043	analytical and immunomicroscopy studies	1005:1043	analytical and immunomicroscopy studies	1005:1043	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	17	48	theme	cellulose	2433:2441	arg1	mobilities					2419:2428	the mobilities	2415:2428	the mobilities of cellulose	2415:2441	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	4	49	theme	present	570:576	arg1	study					578:582	the present study	566:582	the present study	566:582	In the present study, we re-examined this.					
30782133	16	50	theme	cell	2302:2305	arg1	walls					2307:2311	celery collenchyma cell walls	2283:2311	celery collenchyma cell walls	2283:2311	CONCLUSIONS Our studies showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes.					
30782133	15	51	theme	NMR	2124:2126	arg1	spectroscopy					2128:2139	Solid-state NMR spectroscopy	2112:2139	Solid-state NMR spectroscopy	2112:2139	Solid-state NMR spectroscopy showed that particularly cellulose, but also homogalacturonans, decreased in mobility during development.					
30782133	1	52	theme	mechanical	177:186	arg1	support					188:194	mechanical support	177:194	mechanical support for growing organs	177:213	BACKGROUND Collenchyma cells occur widely in eudicotyledons and provide mechanical support for growing organs.					
30782133	8	53	theme	immunomicroscopy	1020:1035	arg1	studies					1037:1043	analytical and immunomicroscopy studies	1005:1043	analytical and immunomicroscopy studies	1005:1043	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	3	54	from	wall	484:487	arg1	celery					492:497	celery	492:497	celery	492:497	A previous study suggested that at least some of the collenchyma cell wall in celery is laid down after expansion has stopped and is thus secondary.					
30782133	6	55	used	used	791:794	arg2	spectroscopy					774:785	solid-state NMR spectroscopy	758:785	solid-state NMR spectroscopy	758:785	Additionally, solid-state NMR spectroscopy was used to examine changes in polysaccharide mobilities during development.					
30782133	12	56	theme	middle	1738:1743	arg1	lamellae					1745:1752	the middle lamellae	1734:1752	the middle lamellae	1734:1752	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	5	57	theme	polysaccharide	681:694	arg1	compositions					696:707	the polysaccharide compositions	677:707	the polysaccharide compositions of these walls during development	677:741	We used chemical analysis and immunomicroscopy to determine changes in the polysaccharide compositions of these walls during development.					
30782133	10	58	theme	lesser	1403:1408	arg1	extent					1410:1415	a lesser extent	1401:1415	a lesser extent	1401:1415	The proportions of cellulose increased, and to a lesser extent those of xyloglucans and heteroxylans.					
30782133	1	59	theme	Collenchyma	116:126	arg1	cells					128:132	BACKGROUND Collenchyma cells	105:132	BACKGROUND Collenchyma cells	105:132	BACKGROUND Collenchyma cells occur widely in eudicotyledons and provide mechanical support for growing organs.					
30782133	13	60	theme	lamella	1894:1900	arg1	junctions					1902:1910	the middle lamellae and middle lamella junctions	1863:1910	the middle lamellae and middle lamella junctions early on	1863:1919	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	11	61	theme	middle	1582:1587	arg1	junctions					1597:1605	middle lamella junctions	1582:1605	middle lamella junctions	1582:1605	Immunomicroscopy showed the homogalacturonans occurred throughout the walls and were most abundant in the middle lamellae and middle lamella junctions.					
30782133	2	62	theme	thick	262:266	arg1	walls					283:287	thick, non-lignified walls	262:287	thick, non-lignified walls	262:287	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	12	63	theme	1 → 5	1696:1700	arg1	-α-L-arabinans					1702:1715	the (1 → 5)-α-L-arabinans	1691:1715	the (1 → 5)-α-L-arabinans	1691:1715	Although the (1 → 4)-β-D-galactans occurred only in the rest of the walls, some of the (1 → 5)-α-L-arabinans also occurred in the middle lamellae and middle lamella junctions.					
30782133	16	64	theme	collenchyma	2290:2300	arg1	walls					2307:2311	celery collenchyma cell walls	2283:2311	celery collenchyma cell walls	2283:2311	CONCLUSIONS Our studies showed that celery collenchyma cell walls are primary and that during their development the polysaccharides undergo dynamic changes.					
30782133	9	65	theme	-α-L-arabinan	1278:1290	arg1	chains					1322:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains	1267:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1267:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	8	66	from	changes	1113:1119	arg1	proportions					1128:1138	the proportions	1124:1138	the proportions of homogalacturonans	1124:1159	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	13	67	theme	middle	1867:1872	arg1	lamellae					1874:1881	middle lamellae	1867:1881	middle lamellae	1867:1881	During development, the location of the xyloglucans changed, being confined to the middle lamellae and middle lamella junctions early on, but later occurred throughout the walls.					
30782133	0	68	from	changes	14:20	arg1	polysaccharides					47:61	collenchyma cell-wall polysaccharides	25:61	collenchyma cell-wall polysaccharides	25:61	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	0	68	from	changes	14:20	arg1	Apium					74:78	Apium	74:78	Apium	74:78	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	0	68	from	changes	14:20	arg1	petioles					95:102	celery (Apium graveolens L.) petioles	66:102	celery (Apium graveolens L.) petioles	66:102	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	5	69	theme	walls	718:722	arg1	compositions					696:707	the polysaccharide compositions	677:707	the polysaccharide compositions of these walls during development	677:741	We used chemical analysis and immunomicroscopy to determine changes in the polysaccharide compositions of these walls during development.					
30782133	8	70	theme	pectic	1068:1073	arg1	polysaccharides					1075:1089	the pectic polysaccharides	1064:1089	the pectic polysaccharides	1064:1089	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	9	71	theme	side	1317:1320	arg1	chains					1322:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains	1267:1327	the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I	1267:1351	There was also a decrease in the proportions of the (1 → 5)-α-L-arabinan and (1 → 4)-β-D-galactan side chains of rhamnogalacturonan I.					
30782133	6	72	from	changes	807:813	arg1	mobilities					833:842	polysaccharide mobilities	818:842	polysaccharide mobilities	818:842	Additionally, solid-state NMR spectroscopy was used to examine changes in polysaccharide mobilities during development.					
30782133	17	73	from	Changes	2404:2410	arg1	mobilities					2419:2428	the mobilities	2415:2428	the mobilities of cellulose	2415:2441	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	0	74	theme	collenchyma	25:35	arg1	polysaccharides					47:61	collenchyma cell-wall polysaccharides	25:61	collenchyma cell-wall polysaccharides	25:61	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	0	75	dep	Apium	74:78	arg1	L.					91:92	Apium graveolens L.	74:92	Apium graveolens L.	74:92	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	10	76	theme	cellulose	1373:1381	arg1	proportions					1358:1368	The proportions	1354:1368	The proportions of cellulose	1354:1381	The proportions of cellulose increased, and to a lesser extent those of xyloglucans and heteroxylans.					
30782133	3	77	theme	collenchyma	467:477	arg1	wall					484:487	the collenchyma cell wall	463:487	the collenchyma cell wall in celery	463:497	A previous study suggested that at least some of the collenchyma cell wall in celery is laid down after expansion has stopped and is thus secondary.					
30782133	14	78	theme	outer	2034:2038	arg1	walls					2040:2044	the outer walls	2030:2044	the outer walls in young cells	2030:2059	The location of the heteroxylans also changed, occurring mostly in the outer walls in young cells, but were more widely distributed in mature cells.					
30782133	17	79	with	consistent	2470:2479	arg1	walls					2495:2499	the cell walls	2486:2499	the cell walls becoming stiffer as expansion ceases	2486:2536	Changes in the mobilities of cellulose and homogalacturonans were consistent with the cell walls becoming stiffer as expansion ceases.					
30782133	6	80	theme	NMR	770:772	arg1	spectroscopy					774:785	solid-state NMR spectroscopy	758:785	solid-state NMR spectroscopy	758:785	Additionally, solid-state NMR spectroscopy was used to examine changes in polysaccharide mobilities during development.					
30782133	0	81	theme	celery	66:71	arg1	petioles					95:102	celery (Apium graveolens L.) petioles	66:102	celery (Apium graveolens L.) petioles	66:102	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	0	81	theme	celery	66:71	arg1	Apium					74:78	Apium	74:78	Apium	74:78	Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles.					
30782133	2	82	theme	pectic	328:333	arg1	polysaccharides					335:349	cellulose and pectic polysaccharides	314:349	cellulose and pectic polysaccharides	314:349	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	7	83	theme	cell	930:933	arg1	expansion					935:943	cell expansion	930:943	cell expansion	930:943	RESULTS We showed the collenchyma walls are deposited only during cell expansion, i.e. they are primary walls.					
30782133	2	84	contain	contain	306:312	arg1	walls					283:287	thick, non-lignified walls	262:287	thick, non-lignified walls	262:287	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	2	84	contain	contain	306:312	arg1	celery					299:304	celery	299:304	celery	299:304	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	2	84	contain	contain	306:312	arg2	polysaccharides					335:349	cellulose and pectic polysaccharides	314:349	cellulose and pectic polysaccharides	314:349	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	2	84	contain	contain	306:312	arg2	walls					283:287	thick, non-lignified walls	262:287	thick, non-lignified walls	262:287	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	14	85	theme	heteroxylans	1983:1994	arg1	location					1967:1974	The location	1963:1974	The location of the heteroxylans	1963:1994	The location of the heteroxylans also changed, occurring mostly in the outer walls in young cells, but were more widely distributed in mature cells.					
30782133	2	86	theme	cellulose	314:322	arg1	polysaccharides					335:349	cellulose and pectic polysaccharides	314:349	cellulose and pectic polysaccharides	314:349	At maturity, the cells are elongated and have thick, non-lignified walls, which in celery contain cellulose and pectic polysaccharides, together with xyloglucans and heteroxylans and heteromannans.					
30782133	8	87	from	decrease	1178:1185	arg1	esterification					1203:1216	their methyl esterification	1190:1216	their methyl esterification	1190:1216	During cell-wall development, analytical and immunomicroscopy studies showed that within the pectic polysaccharides there were no overall changes in the proportions of homogalacturonans, but there was a decrease in their methyl esterification.					
30782133	5	88	from	changes	666:672	arg1	compositions					696:707	the polysaccharide compositions	677:707	the polysaccharide compositions of these walls during development	677:741	We used chemical analysis and immunomicroscopy to determine changes in the polysaccharide compositions of these walls during development.					
29857104	0	0	theme	siRNA	87:91	arg1	delivery					75:82	targeted delivery	66:82	targeted delivery of siRNA to hepatocytes	66:106	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	2	1	from	enzymes	436:442	arg1	liver					451:455	the liver	447:455	the liver	447:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	7	2	theme	confocal	1120:1127	arg1	microscopy					1129:1138	laser confocal microscopy	1114:1138	laser confocal microscopy	1114:1138	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	8	3	theme	potential	1267:1275	arg1	approach					1277:1284	a potential approach	1265:1284	a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes	1265:1394	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	6	4	theme	Kit-8	1057:1061	arg1	assay					1063:1067	the Cell Counting Kit-8 assay	1039:1067	the Cell Counting Kit-8 assay	1039:1067	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	6	5	theme	lower	922:926	arg1	cytotoxicity					928:939	lower cytotoxicity	922:939	lower cytotoxicity in HL-7702 cells	922:956	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	7	6	theme	microscopy	1129:1138	arg1	results					1074:1080	The results	1070:1080	The results of flow cytometric analysis and laser confocal microscopy	1070:1138	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	8	7	theme	nonviral	1323:1330	arg1	derivative					1307:1316	the CTPSA derivative	1297:1316	the CTPSA derivative	1297:1316	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	8	7	theme	nonviral	1323:1330	arg1	vector					1332:1337	a nonviral vector	1321:1337	a nonviral vector for targeted delivery of functional siRNA to hepatocytes	1321:1394	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	6	8	theme	HL-7702	944:950	arg1	cells					952:956	HL-7702 cells	944:956	HL-7702 cells	944:956	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	7	9	theme	NsiRNA	1221:1226	arg1	transfer					1205:1212	the transfer	1201:1212	the transfer of the NsiRNA to HL-7702 cells	1201:1243	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	1	10	theme	FTIR	273:276	arg1	spectroscopy					289:300	FTIR and 1H NMR spectroscopy	273:300	spectroscopy	289:300	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	7	11	theme	laser	1114:1118	arg1	microscopy					1129:1138	laser confocal microscopy	1114:1138	laser confocal microscopy	1114:1138	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	3	12	theme	agarose	478:484	arg1	electrophoresis					490:504	the agarose gel electrophoresis	474:504	the agarose gel electrophoresis	474:504	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	3	13	theme	gel	486:488	arg1	electrophoresis					490:504	the agarose gel electrophoresis	474:504	the agarose gel electrophoresis	474:504	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	5	14	theme	scanning	830:837	arg1	microscopy					848:857	approximately 100 nm using scanning electron microscopy	803:857	approximately 100 nm using scanning electron microscopy	803:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	4	15	theme	zeta	618:621	arg1	potentials					623:632	The zeta potentials	614:632	The zeta potentials	614:632	The zeta potentials and sizes of the complexes were in the range of +8-+15 mv and 120-150 nm, respectively.					
29857104	5	16	with	nanoparticles	763:775	arg1	shape					794:798	a spherical shape	782:798	a spherical shape of approximately 100 nm using scanning electron microscopy	782:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	6	17	theme	Counting	1048:1055	arg1	assay					1063:1067	the Cell Counting Kit-8 assay	1039:1067	the Cell Counting Kit-8 assay	1039:1067	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	5	18	theme	CTPSA/NsiRNA	726:737	arg1	nanoparticles					763:775	nanoparticles	763:775	nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy	763:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	5	18	theme	CTPSA/NsiRNA	726:737	arg1	complex					739:745	The CTPSA/NsiRNA complex	722:745	The CTPSA/NsiRNA complex	722:745	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	7	19	theme	analysis	1101:1108	arg1	results					1074:1080	The results	1070:1080	The results of flow cytometric analysis and laser confocal microscopy	1070:1138	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	2	20	theme	siRNA	375:379	arg1	siRNA					317:321	A nonspecific siRNA	303:321	A nonspecific siRNA (NsiRNA)	303:330	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	20	theme	siRNA	375:379	arg1	molecule					352:359	a model molecule	344:359	a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver	344:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	6	21	from	cytotoxicity	928:939	arg1	cells					952:956	HL-7702 cells	944:956	HL-7702 cells	944:956	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	7	22	theme	HL-7702	1231:1237	arg1	cells					1239:1243	HL-7702 cells	1231:1243	HL-7702 cells	1231:1243	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	8	23	theme	siRNA	1375:1379	arg1	delivery					1352:1359	targeted delivery	1343:1359	targeted delivery of functional siRNA to hepatocytes	1343:1394	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	6	24	theme	CTPSA/NsiRNA	889:900	arg1	complexes					902:910	the CTPSA/NsiRNA complexes	885:910	the CTPSA/NsiRNA complexes	885:910	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	2	25	theme	functional	364:373	arg1	siRNA					375:379	functional siRNA	364:379	functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver	364:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	1	26	theme	1H	282:283	arg1	spectroscopy					289:300	FTIR and 1H NMR spectroscopy	273:300	spectroscopy	289:300	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	4	27	theme	120-150 nm	696:705	arg1	range					673:677	the range	669:677	the range of +8-+15 mv and 120-150 nm	669:705	The zeta potentials and sizes of the complexes were in the range of +8-+15 mv and 120-150 nm, respectively.					
29857104	7	28	theme	cytometric	1090:1099	arg1	analysis					1101:1108	flow cytometric analysis	1085:1108	flow cytometric analysis	1085:1108	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	1	29	theme	3-	192:193	arg1	residues					224:231	3-(dimethylamino)-1-propylamine residues	192:231	3-(dimethylamino)-1-propylamine residues	192:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	0	30	theme	branched	22:29	arg1	derivatives					50:60	cationic branched tea polysaccharide derivatives	13:60	cationic branched tea polysaccharide derivatives	13:60	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	1	31	theme	NMR	285:287	arg1	spectroscopy					289:300	FTIR and 1H NMR spectroscopy	273:300	spectroscopy	289:300	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	8	32	theme	CTPSA	1301:1305	arg1	derivative					1307:1316	the CTPSA derivative	1297:1316	the CTPSA derivative	1297:1316	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	8	32	theme	CTPSA	1301:1305	arg1	vector					1332:1337	a nonviral vector	1321:1337	a nonviral vector for targeted delivery of functional siRNA to hepatocytes	1321:1394	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	2	33	used	used	336:339	arg2	molecule					352:359	a model molecule	344:359	a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver	344:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	33	used	used	336:339	arg2	NsiRNA					324:329	NsiRNA	324:329	NsiRNA	324:329	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	33	used	used	336:339	arg2	siRNA					317:321	A nonspecific siRNA	303:321	A nonspecific siRNA (NsiRNA)	303:330	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	34	theme	nonspecific	305:315	arg1	NsiRNA					324:329	NsiRNA	324:329	NsiRNA	324:329	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	34	theme	nonspecific	305:315	arg1	siRNA					317:321	A nonspecific siRNA	303:321	A nonspecific siRNA (NsiRNA)	303:330	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	34	theme	nonspecific	305:315	arg1	molecule					352:359	a model molecule	344:359	a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver	344:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	35	theme	glycometabolism	420:434	arg1	enzymes					436:442	over-expressed glycometabolism enzymes	405:442	over-expressed glycometabolism enzymes in the liver	405:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	0	36	theme	cationic	13:20	arg1	derivatives					50:60	cationic branched tea polysaccharide derivatives	13:60	cationic branched tea polysaccharide derivatives	13:60	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	3	37	dep	CTPSA	525:529	arg1	the					521:523	the	521:523	the	521:523	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	2	38	theme	over-expressed	405:418	arg1	enzymes					436:442	over-expressed glycometabolism enzymes	405:442	over-expressed glycometabolism enzymes in the liver	405:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	0	39	theme	polysaccharide	35:48	arg1	derivatives					50:60	cationic branched tea polysaccharide derivatives	13:60	cationic branched tea polysaccharide derivatives	13:60	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	4	40	theme	+8-+15 mv	682:690	arg1	range					673:677	the range	669:677	the range of +8-+15 mv and 120-150 nm	669:705	The zeta potentials and sizes of the complexes were in the range of +8-+15 mv and 120-150 nm, respectively.					
29857104	6	41	theme	Cell	1043:1046	arg1	assay					1063:1067	the Cell Counting Kit-8 assay	1039:1067	the Cell Counting Kit-8 assay	1039:1067	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	4	42	theme	complexes	651:659	arg1	sizes					638:642	sizes	638:642	sizes of the complexes	638:659	The zeta potentials and sizes of the complexes were in the range of +8-+15 mv and 120-150 nm, respectively.					
29857104	4	42	theme	complexes	651:659	arg1	potentials					623:632	The zeta potentials	614:632	The zeta potentials	614:632	The zeta potentials and sizes of the complexes were in the range of +8-+15 mv and 120-150 nm, respectively.					
29857104	5	43	theme	electron	839:846	arg1	microscopy					848:857	approximately 100 nm using scanning electron microscopy	803:857	approximately 100 nm using scanning electron microscopy	803:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	0	44	theme	tea	31:33	arg1	derivatives					50:60	cationic branched tea polysaccharide derivatives	13:60	cationic branched tea polysaccharide derivatives	13:60	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	7	45	theme	CTPSA	1159:1163	arg1	derivative					1165:1174	the CTPSA derivative	1155:1174	the CTPSA derivative	1155:1174	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	1	46	theme	-1-propylamine	209:222	arg1	residues					224:231	3-(dimethylamino)-1-propylamine residues	192:231	3-(dimethylamino)-1-propylamine residues	192:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	5	47	theme	spherical	784:792	arg1	shape					794:798	a spherical shape	782:798	a spherical shape of approximately 100 nm using scanning electron microscopy	782:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	6	48	theme	branched	981:988	arg1	bPEI					995:998	bPEI	995:998	bPEI	995:998	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	6	48	theme	branched	981:988	arg1	PEI					990:992	the branched PEI	977:992	the branched PEI (bPEI)	977:999	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	3	49	theme	weight	581:586	arg1	ratio					588:592	their weight ratio	575:592	their weight ratio	575:592	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	8	50	theme	functional	1364:1373	arg1	siRNA					1375:1379	functional siRNA	1364:1379	functional siRNA	1364:1379	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	1	51	theme	cationic	113:120	arg1	derivative					158:167	The cationic branched tea polysaccharide (CTPSA) derivative	109:167	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues	109:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	0	52	theme	derivatives	50:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes	0:106	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	1	53	theme	CTPSA	151:155	arg1	derivative					158:167	The cationic branched tea polysaccharide (CTPSA) derivative	109:167	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues	109:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	1	54	theme	branched	122:129	arg1	derivative					158:167	The cationic branched tea polysaccharide (CTPSA) derivative	109:167	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues	109:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	3	55	from	electrophoresis	490:504	arg1	result					462:467	The result	458:467	The result from the agarose gel electrophoresis	458:504	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	1	56	theme	tea	131:133	arg1	derivative					158:167	The cationic branched tea polysaccharide (CTPSA) derivative	109:167	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues	109:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	3	57	theme	stable	553:558	arg1	complexes					560:568	stable complexes	553:568	stable complexes	553:568	The result from the agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form stable complexes when their weight ratio was larger than 18.					
29857104	8	58	theme	targeted	1343:1350	arg1	delivery					1352:1359	targeted delivery	1343:1359	targeted delivery of functional siRNA to hepatocytes	1343:1394	This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for targeted delivery of functional siRNA to hepatocytes.					
29857104	1	59	theme	polysaccharide	135:148	arg1	derivative					158:167	The cationic branched tea polysaccharide (CTPSA) derivative	109:167	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues	109:231	The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy.					
29857104	7	60	theme	flow	1085:1088	arg1	analysis					1101:1108	flow cytometric analysis	1085:1108	flow cytometric analysis	1085:1108	The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively target the transfer of the NsiRNA to HL-7702 cells.					
29857104	6	61	theme	CTPSA	864:868	arg1	derivative					870:879	The CTPSA derivative	860:879	The CTPSA derivative	860:879	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29857104	5	62	theme	microscopy	848:857	arg1	shape					794:798	a spherical shape	782:798	a spherical shape of approximately 100 nm using scanning electron microscopy	782:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	5	63	theme	using	824:828	arg1	microscopy					848:857	approximately 100 nm using scanning electron microscopy	803:857	approximately 100 nm using scanning electron microscopy	803:857	The CTPSA/NsiRNA complex was observed as nanoparticles with a spherical shape of approximately 100 nm using scanning electron microscopy.					
29857104	0	64	theme	targeted	66:73	arg1	delivery					75:82	targeted delivery	66:82	targeted delivery of siRNA to hepatocytes	66:106	Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.					
29857104	2	65	theme	model	346:350	arg1	siRNA					317:321	A nonspecific siRNA	303:321	A nonspecific siRNA (NsiRNA)	303:330	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	2	65	theme	model	346:350	arg1	molecule					352:359	a model molecule	344:359	a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver	344:455	A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate over-expressed glycometabolism enzymes in the liver.					
29857104	6	66	theme	bPEI/NsiRNA	1005:1015	arg1	complexes					1017:1025	bPEI/NsiRNA complexes	1005:1025	bPEI/NsiRNA complexes	1005:1025	The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared with the branched PEI (bPEI) and bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay.					
29366895	3	0	theme	acacia	279:284	arg1	gum					286:288	acacia gum	279:288	acacia gum	279:288	Many studies were conducted to find out a substitute for acacia gum but very few gum had shown properties as good as acacia gum.					
29366895	10	1	theme	rheological	867:877	arg1	outcome					879:885	The rheological outcome	863:885	The rheological outcome	863:885	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	12	2	with	semblance	1053:1061	arg1	AG					1068:1069	AG	1068:1069	AG	1068:1069	SFG shows semblance with AG and can be use in food and pharmaceutical industry.					
29366895	5	3	theme	functional	534:543	arg1	properties					570:579	physicochemical, functional, rheological and thermal properties	517:579	physicochemical, functional, rheological and thermal properties	517:579	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	4	4	with	solubility	413:422	arg1	viscosity					433:441	low viscosity	429:441	low viscosity	429:441	The exudates collected from Soymida febrifuga also shows high solubility with low viscosity as acacia gum.					
29366895	7	5	theme	monosaccharide	655:668	arg1	composition					670:680	The monosaccharide composition	651:680	The monosaccharide composition of the gums	651:692	The monosaccharide composition of the gums indicated the presence arabinose, galactose, and ribose.					
29366895	3	6	dep	a	262:262	arg1	substitute					264:273	substitute	264:273	substitute	264:273	Many studies were conducted to find out a substitute for acacia gum but very few gum had shown properties as good as acacia gum.					
29366895	2	7	theme	High	146:149	arg1	solubility					151:160	High solubility	146:160	High solubility with low viscosity	146:179	High solubility with low viscosity is one of the best property of this gum.					
29366895	5	8	theme	rheological	546:556	arg1	properties					570:579	physicochemical, functional, rheological and thermal properties	517:579	physicochemical, functional, rheological and thermal properties	517:579	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	3	9	theme	few	299:301	arg1	gum					303:305	very few gum	294:305	very few gum	294:305	Many studies were conducted to find out a substitute for acacia gum but very few gum had shown properties as good as acacia gum.					
29366895	3	10	theme	Many	222:225	arg1	studies					227:233	Many studies	222:233	Many studies	222:233	Many studies were conducted to find out a substitute for acacia gum but very few gum had shown properties as good as acacia gum.					
29366895	11	11	theme	Viscosity	1007:1015	arg1	profile					1017:1023	Viscosity profile	1007:1023	Viscosity profile of both the gums	1007:1040	Alikeness was observed in Viscosity profile of both the gums.					
29366895	12	12	from	use	1082:1084	arg1	food					1089:1092	food	1089:1092	food	1089:1092	SFG shows semblance with AG and can be use in food and pharmaceutical industry.					
29366895	12	12	from	use	1082:1084	arg1	industry					1113:1120	pharmaceutical industry	1098:1120	pharmaceutical industry	1098:1120	SFG shows semblance with AG and can be use in food and pharmaceutical industry.					
29366895	6	13	theme	polysaccharides	634:648	arg1	trend					625:629	a typical trend	615:629	a typical trend of polysaccharides	615:648	The FTIR spectra of SFG revealed a typical trend of polysaccharides.					
29366895	4	14	theme	low	429:431	arg1	viscosity					433:441	low viscosity	429:441	low viscosity	429:441	The exudates collected from Soymida febrifuga also shows high solubility with low viscosity as acacia gum.					
29366895	12	15	theme	pharmaceutical	1098:1111	arg1	industry					1113:1120	pharmaceutical industry	1098:1120	pharmaceutical industry	1098:1120	SFG shows semblance with AG and can be use in food and pharmaceutical industry.					
29366895	1	16	theme	Acacia	92:97	arg1	gum					99:101	Acacia gum	92:101	Acacia gum	92:101	Acacia gum is a well-known and most used exudate gum.					
29366895	1	16	theme	Acacia	92:97	arg1	gum					141:143	a well-known and most used exudate gum	106:143	a well-known and most used exudate gum	106:143	Acacia gum is a well-known and most used exudate gum.					
29366895	0	17	theme	Physicochemical	0:14	arg1	investigation					44:56	Physicochemical, functional and rheological investigation	0:56	Physicochemical, functional and rheological investigation of Soymida	0:67	Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum.					
29366895	5	18	theme	physicochemical	517:531	arg1	properties					570:579	physicochemical, functional, rheological and thermal properties	517:579	physicochemical, functional, rheological and thermal properties	517:579	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	5	19	theme	thermal	562:568	arg1	properties					570:579	physicochemical, functional, rheological and thermal properties	517:579	physicochemical, functional, rheological and thermal properties	517:579	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	6	20	theme	typical	617:623	arg1	trend					625:629	a typical trend	615:629	a typical trend of polysaccharides	615:648	The FTIR spectra of SFG revealed a typical trend of polysaccharides.					
29366895	0	21	theme	functional	17:26	arg1	investigation					44:56	Physicochemical, functional and rheological investigation	0:56	Physicochemical, functional and rheological investigation of Soymida	0:67	Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum.					
29366895	8	22	theme	Element	751:757	arg1	composition					759:769	Element composition	751:769	Element composition of SFG	751:776	Element composition of SFG shows resemblance with AG.					
29366895	11	23	theme	gums	1037:1040	arg1	profile					1017:1023	Viscosity profile	1007:1023	Viscosity profile of both the gums	1007:1040	Alikeness was observed in Viscosity profile of both the gums.					
29366895	10	24	theme	different	938:946	arg1	concentrations					948:961	different concentrations	938:961	different concentrations	938:961	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	2	25	theme	low	167:169	arg1	viscosity					171:179	low viscosity	167:179	low viscosity	167:179	High solubility with low viscosity is one of the best property of this gum.					
29366895	10	26	theme	flow	904:907	arg1	measurements					915:926	flow curve measurements	904:926	flow curve measurements of gum	904:933	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	8	27	theme	SFG	774:776	arg1	composition					759:769	Element composition	751:769	Element composition of SFG	751:776	Element composition of SFG shows resemblance with AG.					
29366895	0	28	theme	rheological	32:42	arg1	investigation					44:56	Physicochemical, functional and rheological investigation	0:56	Physicochemical, functional and rheological investigation of Soymida	0:67	Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum.					
29366895	9	29	theme	molecular	818:826	arg1	less					845:848	less	845:848	less	845:848	However, the molecular weight of SFG is less than the AG.					
29366895	9	29	theme	molecular	818:826	arg1	weight					828:833	the molecular weight	814:833	the molecular weight of SFG	814:840	However, the molecular weight of SFG is less than the AG.					
29366895	4	30	theme	high	408:411	arg1	solubility					413:422	high solubility	408:422	high solubility with low viscosity	408:441	The exudates collected from Soymida febrifuga also shows high solubility with low viscosity as acacia gum.					
29366895	0	31	theme	Soymida	61:67	arg1	investigation					44:56	Physicochemical, functional and rheological investigation	0:56	Physicochemical, functional and rheological investigation of Soymida	0:67	Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum.					
29366895	7	32	dep	presence	708:715	arg1	galactose					728:736	galactose	728:736	galactose	728:736	The monosaccharide composition of the gums indicated the presence arabinose, galactose, and ribose.					
29366895	7	32	dep	presence	708:715	arg1	ribose					743:748	ribose	743:748	ribose	743:748	The monosaccharide composition of the gums indicated the presence arabinose, galactose, and ribose.					
29366895	7	32	dep	presence	708:715	arg1	arabinose					717:725	arabinose	717:725	arabinose	717:725	The monosaccharide composition of the gums indicated the presence arabinose, galactose, and ribose.					
29366895	2	33	theme	property	200:207	arg1	one					184:186	one	184:186	one	184:186	High solubility with low viscosity is one of the best property of this gum.					
29366895	2	33	theme	property	200:207	arg1	property					200:207	the best property	191:207	the best property of this gum	191:219	High solubility with low viscosity is one of the best property of this gum.					
29366895	1	34	theme	well-known	108:117	arg1	gum					141:143	a well-known and most used exudate gum	106:143	a well-known and most used exudate gum	106:143	Acacia gum is a well-known and most used exudate gum.					
29366895	1	34	theme	well-known	108:117	arg1	gum					99:101	Acacia gum	92:101	Acacia gum	92:101	Acacia gum is a well-known and most used exudate gum.					
29366895	10	35	attach	derived	891:897	arg2	outcome					879:885	The rheological outcome	863:885	The rheological outcome	863:885	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	10	35	attach	derived	891:897	arg1	measurements					915:926	flow curve measurements	904:926	flow curve measurements of gum	904:933	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	2	36	theme	best	195:198	arg1	property					200:207	the best property	191:207	the best property of this gum	191:219	High solubility with low viscosity is one of the best property of this gum.					
29366895	2	37	with	solubility	151:160	arg1	viscosity					171:179	low viscosity	167:179	low viscosity	167:179	High solubility with low viscosity is one of the best property of this gum.					
29366895	6	38	theme	SFG	602:604	arg1	spectra					591:597	The FTIR spectra	582:597	The FTIR spectra of SFG	582:604	The FTIR spectra of SFG revealed a typical trend of polysaccharides.					
29366895	10	39	theme	curve	909:913	arg1	measurements					915:926	flow curve measurements	904:926	flow curve measurements of gum	904:933	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	9	40	theme	SFG	838:840	arg1	less					845:848	less	845:848	less	845:848	However, the molecular weight of SFG is less than the AG.					
29366895	9	40	theme	SFG	838:840	arg1	weight					828:833	the molecular weight	814:833	the molecular weight of SFG	814:840	However, the molecular weight of SFG is less than the AG.					
29366895	10	41	theme	gum	931:933	arg1	measurements					915:926	flow curve measurements	904:926	flow curve measurements of gum	904:933	The rheological outcome was derived from flow curve measurements of gum at different concentrations and temperatures.					
29366895	11	42	located	observed	995:1002	arg2	Alikeness					981:989	Alikeness	981:989	Alikeness	981:989	Alikeness was observed in Viscosity profile of both the gums.					
29366895	11	42	located	observed	995:1002	arg1	profile					1017:1023	Viscosity profile	1007:1023	Viscosity profile of both the gums	1007:1040	Alikeness was observed in Viscosity profile of both the gums.					
29366895	4	43	theme	Soymida	379:385	arg1	febrifuga					387:395	Soymida febrifuga	379:395	Soymida febrifuga	379:395	The exudates collected from Soymida febrifuga also shows high solubility with low viscosity as acacia gum.					
29366895	5	44	theme	Purified	458:465	arg1	SFG					490:492	SFG	490:492	SFG	490:492	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	5	44	theme	Purified	458:465	arg1	gum					485:487	Soymida febrifuga gum	467:487	Purified Soymida febrifuga gum (SFG)	458:493	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	1	45	theme	used	128:131	arg1	gum					141:143	a well-known and most used exudate gum	106:143	a well-known and most used exudate gum	106:143	Acacia gum is a well-known and most used exudate gum.					
29366895	1	45	theme	used	128:131	arg1	gum					99:101	Acacia gum	92:101	Acacia gum	92:101	Acacia gum is a well-known and most used exudate gum.					
29366895	0	46	theme	exudate	79:85	arg1	gum					87:89	exudate gum	79:89	exudate gum	79:89	Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum.					
29366895	5	47	theme	Soymida	467:473	arg1	SFG					490:492	SFG	490:492	SFG	490:492	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	5	47	theme	Soymida	467:473	arg1	gum					485:487	Soymida febrifuga gum	467:487	Purified Soymida febrifuga gum (SFG)	458:493	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	1	48	theme	exudate	133:139	arg1	gum					141:143	a well-known and most used exudate gum	106:143	a well-known and most used exudate gum	106:143	Acacia gum is a well-known and most used exudate gum.					
29366895	1	48	theme	exudate	133:139	arg1	gum					99:101	Acacia gum	92:101	Acacia gum	92:101	Acacia gum is a well-known and most used exudate gum.					
29366895	6	49	theme	FTIR	586:589	arg1	spectra					591:597	The FTIR spectra	582:597	The FTIR spectra of SFG	582:604	The FTIR spectra of SFG revealed a typical trend of polysaccharides.					
29366895	7	50	theme	gums	689:692	arg1	composition					670:680	The monosaccharide composition	651:680	The monosaccharide composition of the gums	651:692	The monosaccharide composition of the gums indicated the presence arabinose, galactose, and ribose.					
29366895	3	51	theme	acacia	339:344	arg1	gum					346:348	acacia gum	339:348	acacia gum	339:348	Many studies were conducted to find out a substitute for acacia gum but very few gum had shown properties as good as acacia gum.					
29366895	4	52	theme	acacia	446:451	arg1	gum					453:455	acacia gum	446:455	acacia gum	446:455	The exudates collected from Soymida febrifuga also shows high solubility with low viscosity as acacia gum.					
29366895	2	53	theme	gum	217:219	arg1	property					200:207	the best property	191:207	the best property of this gum	191:219	High solubility with low viscosity is one of the best property of this gum.					
29366895	5	54	theme	febrifuga	475:483	arg1	SFG					490:492	SFG	490:492	SFG	490:492	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29366895	5	54	theme	febrifuga	475:483	arg1	gum					485:487	Soymida febrifuga gum	467:487	Purified Soymida febrifuga gum (SFG)	458:493	Purified Soymida febrifuga gum (SFG) was characterised for physicochemical, functional, rheological and thermal properties.					
29655727	0	0	theme	liquid	82:87	arg1	spectrometry					116:127	liquid chromatography-tandem mass spectrometry	82:127	liquid chromatography-tandem mass spectrometry	82:127	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	2	1	theme	critical	361:368	arg1	processes					401:409	critical physiological and pathological processes	361:409	critical physiological and pathological processes	361:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	0	2	from	profiling	22:30	arg1	stages					59:64	different stages	49:64	different stages of lactation	49:77	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	1	3	theme	biological	176:185	arg1	functions					187:195	several important biological functions	158:195	several important biological functions that promote infant health	158:222	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	0	4	theme	mass	111:114	arg1	spectrometry					116:127	liquid chromatography-tandem mass spectrometry	82:127	liquid chromatography-tandem mass spectrometry	82:127	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	3	5	theme	spectrometry	587:598	arg1	approach					600:607	a liquid chromatography-tandem mass spectrometry approach	551:607	a liquid chromatography-tandem mass spectrometry approach	551:607	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	6	theme	liquid	553:558	arg1	spectrometry					587:598	a liquid chromatography-tandem mass spectrometry	551:598	a liquid chromatography-tandem mass spectrometry approach	551:607	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	0	7	theme	chromatography-tandem	89:109	arg1	spectrometry					116:127	liquid chromatography-tandem mass spectrometry	82:127	liquid chromatography-tandem mass spectrometry	82:127	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	4	8	theme	chondroitin	750:760	arg1	sulfate					762:768	chondroitin sulfate	750:768	chondroitin sulfate	750:768	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	2	9	theme	complex	287:293	arg1	family					295:300	a complex family	285:300	a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes	285:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	2	9	theme	complex	287:293	arg1	glycosaminoglycans					255:272	glycosaminoglycans	255:272	glycosaminoglycans (GAGs)	255:279	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	6	10	theme	lactation	958:966	arg1	stages					948:953	different stages	938:953	different stages of lactation	938:966	This study provided an important guide for the demands of GAGs during different stages of lactation.					
29655727	4	11	theme	heparan	733:739	arg1	sulfate					741:747	heparan sulfate	733:747	heparan sulfate	733:747	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	3	12	theme	lactation	531:539	arg1	stages					521:526	different stages	511:526	different stages of lactation	511:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	4	13	theme	milk	681:684	arg1	samples					686:692	the human milk samples	671:692	the human milk samples	671:692	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	7	14	dep	composition	1021:1031	arg1	the					1017:1019	the	1017:1019	the	1017:1019	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	5	15	theme	extending	807:815	arg1	lactation					797:805	lactation extending	797:815	lactation extending	797:815	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	3	16	theme	detailed	447:454	arg1	composition					456:466	the detailed composition	443:466	the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation	443:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	7	17	theme	infant	1062:1067	arg1	formula					1069:1075	infant formula	1062:1075	infant formula	1062:1075	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	3	18	theme	chromatography-tandem	560:580	arg1	spectrometry					587:598	a liquid chromatography-tandem mass spectrometry	551:598	a liquid chromatography-tandem mass spectrometry approach	551:607	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	2	19	theme	processes	401:409	arg1	variety					350:356	a variety	348:356	a variety of critical physiological and pathological processes	348:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	2	19	theme	processes	401:409	arg1	processes					401:409	critical physiological and pathological processes	361:409	critical physiological and pathological processes	361:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	0	20	theme	Glycosaminoglycanomic	0:20	arg1	profiling					22:30	Glycosaminoglycanomic profiling	0:30	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry	0:127	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	7	21	theme	formula	1069:1075	arg1	composition					1021:1031	composition	1021:1031	composition	1021:1031	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	7	21	theme	formula	1069:1075	arg1	properties					1048:1057	functional properties	1037:1057	functional properties	1037:1057	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	1	22	from	Glycans	130:136	arg1	milk					147:150	human milk	141:150	human milk	141:150	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	2	23	theme	carbohydrate	317:328	arg1	family					295:300	a complex family	285:300	a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes	285:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	2	23	theme	carbohydrate	317:328	arg1	glycosaminoglycans					255:272	glycosaminoglycans	255:272	glycosaminoglycans (GAGs)	255:279	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	4	24	from	fraction	659:666	arg1	samples					686:692	the human milk samples	671:692	the human milk samples	671:692	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	1	25	theme	infant	210:215	arg1	health					217:222	infant health	210:222	infant health	210:222	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	0	26	theme	human	35:39	arg1	milk					41:44	human milk	35:44	human milk	35:44	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	3	27	from	mothers	500:506	arg1	content					431:437	content	431:437	content	431:437	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	27	from	mothers	500:506	arg1	stages					521:526	different stages	511:526	different stages of lactation	511:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	27	from	mothers	500:506	arg1	GAGs					482:485	human milk GAGs	471:485	human milk GAGs from Chinese mothers in different stages of lactation	471:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	27	from	mothers	500:506	arg1	composition					456:466	the detailed composition	443:466	the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation	443:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	2	28	theme	polyanionic	305:315	arg1	carbohydrate					317:328	polyanionic carbohydrate	305:328	polyanionic carbohydrate	305:328	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	3	29	theme	human	471:475	arg1	GAGs					482:485	human milk GAGs	471:485	human milk GAGs from Chinese mothers in different stages of lactation	471:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	30	theme	mass	582:585	arg1	spectrometry					587:598	a liquid chromatography-tandem mass spectrometry	551:598	a liquid chromatography-tandem mass spectrometry approach	551:607	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	6	31	theme	different	938:946	arg1	stages					948:953	different stages	938:953	different stages of lactation	938:966	This study provided an important guide for the demands of GAGs during different stages of lactation.					
29655727	3	32	theme	different	511:519	arg1	stages					521:526	different stages	511:526	different stages of lactation	511:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	33	theme	milk	477:480	arg1	GAGs					482:485	human milk GAGs	471:485	human milk GAGs from Chinese mothers in different stages of lactation	471:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	34	dep	content	431:437	arg1	the					427:429	the	427:429	the	427:429	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	2	35	theme	pathological	388:399	arg1	processes					401:409	critical physiological and pathological processes	361:409	critical physiological and pathological processes	361:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	0	36	theme	milk	41:44	arg1	profiling					22:30	Glycosaminoglycanomic profiling	0:30	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry	0:127	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	6	37	theme	GAGs	926:929	arg1	demands					915:921	the demands	911:921	the demands of GAGs	911:929	This study provided an important guide for the demands of GAGs during different stages of lactation.					
29655727	7	38	theme	functional	1037:1046	arg1	properties					1048:1057	functional properties	1037:1057	functional properties	1037:1057	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	2	39	theme	glycans	246:252	arg1	kind					228:231	kind	228:231	kind of important glycans	228:252	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	3	40	from	composition	456:466	arg1	mothers					500:506	Chinese mothers	492:506	Chinese mothers in different stages of lactation	492:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	4	41	theme	GAG	655:657	arg1	fraction					659:666	the GAG fraction	651:666	the GAG fraction in the human milk samples	651:692	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	4	41	theme	GAG	655:657	arg1	complex					703:709	complex	703:709	complex	703:709	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	2	42	theme	important	236:244	arg1	glycans					246:252	important glycans	236:252	important glycans	236:252	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
29655727	3	43	theme	Chinese	492:498	arg1	mothers					500:506	Chinese mothers	492:506	Chinese mothers in different stages of lactation	492:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	0	44	theme	different	49:57	arg1	stages					59:64	different stages	49:64	different stages of lactation	49:77	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	6	45	theme	important	891:899	arg1	guide					901:905	an important guide	888:905	an important guide for the demands of GAGs	888:929	This study provided an important guide for the demands of GAGs during different stages of lactation.					
29655727	4	46	theme	human	675:679	arg1	samples					686:692	the human milk samples	671:692	the human milk samples	671:692	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	5	47	from	amount	828:833	arg1	milk					852:855	human milk	846:855	human milk	846:855	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	1	48	theme	human	141:145	arg1	milk					147:150	human milk	141:150	human milk	141:150	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	7	49	from	studies	1006:1012	arg1	composition					1021:1031	composition	1021:1031	composition	1021:1031	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	7	49	from	studies	1006:1012	arg1	properties					1048:1057	functional properties	1037:1057	functional properties	1037:1057	The results were also beneficial for studies on the composition and functional properties of infant formula.					
29655727	4	50	theme	hyaluronic	775:784	arg1	acid					786:789	hyaluronic acid	775:789	hyaluronic acid	775:789	The results showed that the GAG fraction in the human milk samples was very complex as it was composed of heparan sulfate, chondroitin sulfate, and hyaluronic acid.					
29655727	3	51	from	content	431:437	arg1	mothers					500:506	Chinese mothers	492:506	Chinese mothers in different stages of lactation	492:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	1	52	theme	several	158:164	arg1	functions					187:195	several important biological functions	158:195	several important biological functions that promote infant health	158:222	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	3	53	theme	GAGs	482:485	arg1	content					431:437	content	431:437	content	431:437	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	3	53	theme	GAGs	482:485	arg1	composition					456:466	the detailed composition	443:466	the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation	443:539	In this study, the content and the detailed composition of human milk GAGs from Chinese mothers in different stages of lactation, based on a liquid chromatography-tandem mass spectrometry approach was investigated.					
29655727	0	54	theme	lactation	69:77	arg1	stages					59:64	different stages	49:64	different stages of lactation	49:77	Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.					
29655727	5	55	theme	GAGs	838:841	arg1	GAGs					838:841	GAGs	838:841	GAGs	838:841	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	5	55	theme	GAGs	838:841	arg1	amount					828:833	the total amount	818:833	the total amount of GAGs in human milk	818:855	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	5	56	theme	total	822:826	arg1	GAGs					838:841	GAGs	838:841	GAGs	838:841	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	5	56	theme	total	822:826	arg1	amount					828:833	the total amount	818:833	the total amount of GAGs in human milk	818:855	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	5	57	theme	human	846:850	arg1	milk					852:855	human milk	846:855	human milk	846:855	With lactation extending, the total amount of GAGs in human milk decreased.					
29655727	1	58	theme	important	166:174	arg1	functions					187:195	several important biological functions	158:195	several important biological functions that promote infant health	158:222	Glycans in human milk serve several important biological functions that promote infant health.					
29655727	2	59	theme	physiological	370:382	arg1	processes					401:409	critical physiological and pathological processes	361:409	critical physiological and pathological processes	361:409	As kind of important glycans, glycosaminoglycans (GAGs) are a complex family of polyanionic carbohydrate, participating in a variety of critical physiological and pathological processes.					
31445150	3	0	theme	physicochemical	641:655	arg1	property					672:679	physicochemical and structural property	641:679	physicochemical and structural property	641:679	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	3	0	theme	physicochemical	641:655	arg1	compositions					627:638	the chemical compositions	614:638	the chemical compositions	614:638	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	2	1	theme	48.30 ± 0.6	537:547	arg1	%					548:548	%	548:548	%	548:548	Based on it, 48.30 ± 0.6% yield of crude polysaccharide was obtained.					
31445150	1	2	theme	50 min	476:481	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	2	theme	50 min	476:481	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	2	theme	50 min	476:481	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	2	theme	50 min	476:481	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	3	theme	present	152:158	arg1	study					160:164	the present study	148:164	the present study	148:164	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	4	theme	polysaccharides	94:108	arg1	characterization					48:63	characterization	48:63	characterization	48:63	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	4	theme	polysaccharides	94:108	arg1	activities					80:89	biological activities	69:89	biological activities	69:89	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	4	theme	polysaccharides	94:108	arg1	extraction					36:45	ultrasonic-assisted extraction	16:45	ultrasonic-assisted extraction	16:45	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	3	5	theme	chemical	618:625	arg1	property					672:679	physicochemical and structural property	641:679	physicochemical and structural property	641:679	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	3	5	theme	chemical	618:625	arg1	compositions					627:638	the chemical compositions	614:638	the chemical compositions	614:638	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	1	6	theme	optimum	355:361	arg1	conditions					363:372	the optimum conditions	351:372	the optimum conditions	351:372	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	7	theme	chusua	122:127	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	7	theme	chusua	122:127	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	1	8	dep	optimized	261:269	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	8	dep	optimized	261:269	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	8	dep	optimized	261:269	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	8	dep	optimized	261:269	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	6	9	theme	fractions	1113:1121	arg1	power					1063:1067	power	1063:1067	power	1063:1067	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	6	9	theme	fractions	1113:1121	arg1	activities					1085:1094	antioxidant activities	1073:1094	antioxidant activities	1073:1094	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	0	10	theme	Orchis	115:120	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	10	theme	Orchis	115:120	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	1	11	theme	Box-Behnken	325:335	arg1	design					337:342	a Box-Behnken design	323:342	a Box-Behnken design (BBD)	323:348	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	11	theme	Box-Behnken	325:335	arg1	BBD					345:347	BBD	345:347	BBD	345:347	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	12	theme	concentration-dependent	1390:1412	arg1	manner					1414:1419	a concentration-dependent manner	1388:1419	a concentration-dependent manner	1388:1419	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	1	13	theme	ultrasonic	397:406	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	6	14	theme	purified	1104:1111	arg1	fractions					1113:1121	both purified fractions	1099:1121	both purified fractions	1099:1121	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	8	15	theme	food	1522:1525	arg1	industry					1527:1534	the pharmaceutical and food industry	1499:1534	industry	1527:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	4	16	theme	average	869:875	arg1	weight					887:892	the average molecular weight	865:892	the average molecular weight	865:892	The results showed that USP-U and USP-N were hetero-polysaccharides, mainly composed of mannose and glucose, and the average molecular weight was 25.57 kDa and 11.64 kDa, respectively.					
31445150	4	16	theme	average	869:875	arg1	25.57 kDa					898:906	25.57 kDa	898:906	25.57 kDa	898:906	The results showed that USP-U and USP-N were hetero-polysaccharides, mainly composed of mannose and glucose, and the average molecular weight was 25.57 kDa and 11.64 kDa, respectively.					
31445150	8	17	theme	efficacy	1480:1487	arg1	factor					1489:1494	a new efficacy factor	1474:1494	a new efficacy factor in the pharmaceutical and food industry	1474:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	8	17	theme	efficacy	1480:1487	arg1	polysaccharide					1428:1441	Salep polysaccharide	1422:1441	Salep polysaccharide	1422:1441	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	2	18	theme	crude	559:563	arg1	polysaccharide					565:578	crude polysaccharide	559:578	crude polysaccharide	559:578	Based on it, 48.30 ± 0.6% yield of crude polysaccharide was obtained.					
31445150	2	19	theme	polysaccharide	565:578	arg1	yield					550:554	48.30 ± 0.6% yield	537:554	48.30 ± 0.6% yield of crude polysaccharide	537:578	Based on it, 48.30 ± 0.6% yield of crude polysaccharide was obtained.					
31445150	1	20	theme	water/raw	487:495	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	5	21	theme	α-linkage	998:1006	arg1	presence					986:993	the presence	982:993	the presence of α-linkage	982:1006	FT-IR spectra and NMR results indicated that the presence of α-linkage glycopyranose via typical peaks.					
31445150	0	22	theme	D.	129:130	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	22	theme	D.	129:130	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	23	from	characterization	48:63	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	23	from	characterization	48:63	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	1	24	theme	material	497:504	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	25	theme	ultrasonic-assisted	167:185	arg1	extraction					187:196	ultrasonic-assisted extraction	167:196	ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep)	167:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	25	theme	ultrasonic-assisted	167:185	arg1	UAE					199:201	UAE	199:201	UAE	199:201	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	26	theme	effective	1319:1327	arg1	concentration					1329:1341	their effective concentration	1313:1341	their effective concentration	1313:1341	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	3	27	theme	structural	661:670	arg1	property					672:679	physicochemical and structural property	641:679	physicochemical and structural property	641:679	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	3	27	theme	structural	661:670	arg1	compositions					627:638	the chemical compositions	614:638	the chemical compositions	614:638	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	1	28	theme	390 W	417:421	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	28	theme	390 W	417:421	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	28	theme	390 W	417:421	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	28	theme	390 W	417:421	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	29	theme	potent	1351:1356	arg1	activity					1376:1383	potent anti-inflammatory activity	1351:1383	potent anti-inflammatory activity	1351:1383	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	8	30	theme	new	1476:1478	arg1	factor					1489:1494	a new efficacy factor	1474:1494	a new efficacy factor in the pharmaceutical and food industry	1474:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	8	30	theme	new	1476:1478	arg1	polysaccharide					1428:1441	Salep polysaccharide	1422:1441	Salep polysaccharide	1422:1441	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	7	31	contain	had	1347:1349	arg1	polysaccharides					1235:1249	purified polysaccharides	1226:1249	purified polysaccharides	1226:1249	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	7	31	contain	had	1347:1349	arg2	activity					1376:1383	potent anti-inflammatory activity	1351:1383	potent anti-inflammatory activity	1351:1383	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	2	32	theme	%	548:548	arg1	yield					550:554	48.30 ± 0.6% yield	537:554	48.30 ± 0.6% yield of crude polysaccharide	537:578	Based on it, 48.30 ± 0.6% yield of crude polysaccharide was obtained.					
31445150	5	33	theme	NMR	955:957	arg1	results					959:965	NMR results	955:965	NMR results	955:965	FT-IR spectra and NMR results indicated that the presence of α-linkage glycopyranose via typical peaks.					
31445150	7	34	theme	obvious	1259:1265	arg1	cytotoxicity					1267:1278	no obvious cytotoxicity	1256:1278	no obvious cytotoxicity	1256:1278	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	0	35	theme	extraction	36:45	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).	0:143	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	7	36	theme	anti-inflammatory	1358:1374	arg1	activity					1376:1383	potent anti-inflammatory activity	1351:1383	potent anti-inflammatory activity	1351:1383	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	8	37	theme	Salep	1422:1426	arg1	factor					1489:1494	a new efficacy factor	1474:1494	a new efficacy factor in the pharmaceutical and food industry	1474:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	8	37	theme	Salep	1422:1426	arg1	polysaccharide					1428:1441	Salep polysaccharide	1422:1441	Salep polysaccharide	1422:1441	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	1	38	theme	extraction	424:433	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	39	theme	40 mL/g	515:521	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	39	theme	40 mL/g	515:521	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	39	theme	40 mL/g	515:521	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	39	theme	40 mL/g	515:521	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	40	theme	ultrasonic-assisted	16:34	arg1	extraction					36:45	ultrasonic-assisted extraction	16:45	ultrasonic-assisted extraction	16:45	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	6	41	theme	antioxidant	1073:1083	arg1	activities					1085:1094	antioxidant activities	1073:1094	antioxidant activities	1073:1094	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	0	42	theme	characterization	48:63	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).	0:143	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	1	43	theme	response	274:281	arg1	methodology					291:301	response surface methodology	274:301	response surface methodology (RSM) combined with a Box-Behnken design (BBD)	274:348	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	43	theme	response	274:281	arg1	RSM					304:306	RSM	304:306	RSM	304:306	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	44	from	Don	245:247	arg1	extraction					187:196	ultrasonic-assisted extraction	167:196	ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep)	167:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	44	from	Don	245:247	arg1	UAE					199:201	UAE	199:201	UAE	199:201	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	44	from	Don	245:247	arg1	polysaccharides					207:221	polysaccharides	207:221	polysaccharides from Orchis chusua D. Don (salep)	207:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	6	45	theme	free	1168:1171	arg1	radicals					1173:1180	free radicals	1168:1180	free radicals	1168:1180	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	1	46	theme	surface	283:289	arg1	methodology					291:301	response surface methodology	274:301	response surface methodology (RSM) combined with a Box-Behnken design (BBD)	274:348	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	46	theme	surface	283:289	arg1	RSM					304:306	RSM	304:306	RSM	304:306	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	47	contain	have	1251:1254	arg1	polysaccharides					1235:1249	purified polysaccharides	1226:1249	purified polysaccharides	1226:1249	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	7	47	contain	have	1251:1254	arg2	cytotoxicity					1267:1278	no obvious cytotoxicity	1256:1278	no obvious cytotoxicity	1256:1278	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	5	48	theme	FT-IR	937:941	arg1	spectra					943:949	FT-IR spectra	937:949	FT-IR spectra	937:949	FT-IR spectra and NMR results indicated that the presence of α-linkage glycopyranose via typical peaks.					
31445150	1	49	theme	polysaccharides	207:221	arg1	extraction					187:196	ultrasonic-assisted extraction	167:196	ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep)	167:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	49	theme	polysaccharides	207:221	arg1	UAE					199:201	UAE	199:201	UAE	199:201	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	50	theme	biological	69:78	arg1	activities					80:89	biological activities	69:89	biological activities	69:89	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	51	from	activities	80:89	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	51	from	activities	80:89	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	5	52	theme	typical	1026:1032	arg1	peaks					1034:1038	typical peaks	1026:1038	typical peaks	1026:1038	FT-IR spectra and NMR results indicated that the presence of α-linkage glycopyranose via typical peaks.					
31445150	6	53	theme	reducing	1054:1061	arg1	power					1063:1067	power	1063:1067	power	1063:1067	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	8	54	from	factor	1489:1494	arg1	industry					1527:1534	the pharmaceutical and food industry	1499:1534	industry	1527:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	1	55	from	extraction	187:196	arg1	salep					250:254	salep	250:254	salep	250:254	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	55	from	extraction	187:196	arg1	Don					245:247	Orchis chusua D. Don	228:247	Orchis chusua D. Don (salep)	228:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	56	from	Don	132:134	arg1	polysaccharides					94:108	polysaccharides	94:108	polysaccharides from Orchis chusua D. Don (Salep)	94:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	56	from	Don	132:134	arg1	characterization					48:63	characterization	48:63	characterization	48:63	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	56	from	Don	132:134	arg1	activities					80:89	biological activities	69:89	biological activities	69:89	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	56	from	Don	132:134	arg1	extraction					36:45	ultrasonic-assisted extraction	16:45	ultrasonic-assisted extraction	16:45	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	4	57	theme	molecular	877:885	arg1	weight					887:892	the average molecular weight	865:892	the average molecular weight	865:892	The results showed that USP-U and USP-N were hetero-polysaccharides, mainly composed of mannose and glucose, and the average molecular weight was 25.57 kDa and 11.64 kDa, respectively.					
31445150	4	57	theme	molecular	877:885	arg1	25.57 kDa					898:906	25.57 kDa	898:906	25.57 kDa	898:906	The results showed that USP-U and USP-N were hetero-polysaccharides, mainly composed of mannose and glucose, and the average molecular weight was 25.57 kDa and 11.64 kDa, respectively.					
31445150	1	58	theme	60 °C	450:454	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	58	theme	60 °C	450:454	arg1	temperature					435:445	extraction temperature	424:445	extraction temperature of 60 °C	424:454	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	58	theme	60 °C	450:454	arg1	power					408:412	ultrasonic power	397:412	ultrasonic power of 390 W	397:421	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	58	theme	60 °C	450:454	arg1	ratio					506:510	water/raw material ratio	487:510	water/raw material ratio of 40 mL/g	487:521	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	3	59	dep	red	742:744	arg1	test					746:749	test	746:749	test	746:749	After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test.					
31445150	6	60	theme	high	1143:1146	arg1	ability					1148:1154	a relatively high ability	1130:1154	a relatively high ability to scavenge free radicals	1130:1180	Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals.					
31445150	7	61	theme	L6	1301:1302	arg1	cells					1304:1308	L6 cells	1301:1308	L6 cells	1301:1308	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	1	62	theme	extraction	457:466	arg1	time					468:471	extraction time	457:471	extraction time of 50 min	457:481	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	63	theme	cytotoxicity	1195:1206	arg1	assay					1208:1212	the cytotoxicity assay	1191:1212	the cytotoxicity assay	1191:1212	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	1	64	theme	Orchis	228:233	arg1	salep					250:254	salep	250:254	salep	250:254	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	64	theme	Orchis	228:233	arg1	Don					245:247	Orchis chusua D. Don	228:247	Orchis chusua D. Don (salep)	228:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	7	65	theme	purified	1226:1233	arg1	polysaccharides					1235:1249	purified polysaccharides	1226:1249	purified polysaccharides	1226:1249	Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner.					
31445150	8	66	theme	pharmaceutical	1503:1516	arg1	industry					1527:1534	the pharmaceutical and food industry	1499:1534	industry	1527:1534	Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry.					
31445150	1	67	theme	chusua	235:240	arg1	salep					250:254	salep	250:254	salep	250:254	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	67	theme	chusua	235:240	arg1	Don					245:247	Orchis chusua D. Don	228:247	Orchis chusua D. Don (salep)	228:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	0	68	theme	activities	80:89	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).	0:143	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	69	from	extraction	36:45	arg1	Salep					137:141	Salep	137:141	Salep	137:141	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	0	69	from	extraction	36:45	arg1	Don					132:134	Orchis chusua D. Don	115:134	Orchis chusua D. Don (Salep)	115:142	Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).					
31445150	1	70	theme	D.	242:243	arg1	salep					250:254	salep	250:254	salep	250:254	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31445150	1	70	theme	D.	242:243	arg1	Don					245:247	Orchis chusua D. Don	228:247	Orchis chusua D. Don (salep)	228:255	In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 °C, extraction time of 50 min and water/raw material ratio of 40 mL/g.					
31010286	0	0	theme	Kdo-lipid	94:102	arg1	Backbone					106:113	a Bacterial Kdo-lipid A Backbone	82:113	a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike	82:205	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	1	1	theme	spike	377:381	arg1	glycoprotein					364:375	the HIV-1 envelope glycoprotein spike	345:381	the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	345:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	1	theme	spike	377:381	arg1	targets					392:398	targets	392:398	targets for broadly neutralizing antibodies	392:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	5	2	theme	mannotriosyl	1060:1071	arg1	donor					1094:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	7	3	theme	Global	1328:1333	arg1	deprotection					1335:1346	Global deprotection	1328:1346	Global deprotection	1328:1346	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	4	4	theme	byproducts	974:983	arg1	formation					944:952	formation	944:952	formation of unwanted imidate byproducts	944:983	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	6	5	theme	mannotriosyl	1217:1228	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	0	6	theme	Bacterial	84:92	arg1	Backbone					106:113	a Bacterial Kdo-lipid A Backbone	82:113	a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike	82:205	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	0	7	theme	Envelope	192:199	arg1	Spike					201:205	the HIV-1 Envelope Spike	182:205	the HIV-1 Envelope Spike	182:205	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	5	8	theme	Blockwise	986:994	arg1	coupling					996:1003	Blockwise coupling	986:1003	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	986:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	2	9	theme	oligomannose-specific	553:573	arg1	antibodies					592:601	oligomannose-specific HIV-neutralizing antibodies	553:601	oligomannose-specific HIV-neutralizing antibodies	553:601	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	5	10	theme	trichloroacetimidate	1073:1092	arg1	donor					1094:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	4	11	theme	unwanted	957:964	arg1	byproducts					974:983	unwanted imidate byproducts	957:983	unwanted imidate byproducts	957:983	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	6	12	theme	trichloroacetimidate	1230:1249	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	4	13	theme	larger	842:847	arg1	glycans					849:855	larger glycans	842:855	larger glycans	842:855	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	0	14	theme	A	104:104	arg1	Backbone					106:113	a Bacterial Kdo-lipid A Backbone	82:113	a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike	82:205	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	2	15	theme	LOS	642:644	arg1	structure					646:654	the Rv3 LOS structure	634:654	the Rv3 LOS structure	634:654	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	4	16	theme	N-acetylamino	862:874	arg1	groups					876:881	the N-acetylamino groups	858:881	the N-acetylamino groups of the glucosamine units	858:906	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	4	16	theme	N-acetylamino	862:874	arg1	units					902:906	the glucosamine units	886:906	the glucosamine units	886:906	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	6	17	link	-linked	1209:1215	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	3	18	theme	innermost	807:815	arg1	residue					821:827	the innermost Kdo residue	803:827	the innermost Kdo residue	803:827	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	6	19	theme	donor	1251:1255	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	1	20	gly	glycoprotein	364:375	arg1	glycoprotein					364:375	the HIV-1 envelope glycoprotein spike	345:381	the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	345:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	20	gly	glycoprotein	364:375	arg1	targets					392:398	targets	392:398	targets for broadly neutralizing antibodies	392:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	6	21	theme	mannose	1178:1184	arg1	O-6					1149:1151	O-6	1149:1151	O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1149:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	2	22	theme	strategy	534:541	arg1	part					509:512	part	509:512	part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies	509:601	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	5	23	link	-linked	1052:1058	arg1	donor					1094:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	1	24	from	oligomannoses	328:340	arg1	glycoprotein					364:375	the HIV-1 envelope glycoprotein spike	345:381	the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	345:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	24	from	oligomannoses	328:340	arg1	targets					392:398	targets	392:398	targets for broadly neutralizing antibodies	392:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	2	25	theme	viral	477:481	arg1	epitopes					497:504	viral and bacterial epitopes	477:504	viral and bacterial epitopes	477:504	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	8	26	theme	representative	1493:1506	arg1	antibodies					1547:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	However, representative oligomannose-specific HIV-neutralizing antibodies bound the undecasaccharide conjugates poorly.					
31010286	6	27	theme	branching	1168:1176	arg1	mannose					1178:1184	the central branching mannose	1156:1184	the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1156:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	1	28	theme	neutralizing	412:423	arg1	antibodies					425:434	broadly neutralizing antibodies	404:434	broadly neutralizing antibodies	404:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	5	29	with	coupling	996:1003	arg1	donor					1094:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	5	30	theme	acceptor	1028:1035	arg1	coupling					996:1003	Blockwise coupling	986:1003	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	986:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	8	31	theme	undecasaccharide	1568:1583	arg1	conjugates					1585:1594	the undecasaccharide conjugates	1564:1594	the undecasaccharide conjugates	1564:1594	However, representative oligomannose-specific HIV-neutralizing antibodies bound the undecasaccharide conjugates poorly.					
31010286	6	32	theme	central	1160:1166	arg1	mannose					1178:1184	the central branching mannose	1156:1184	the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1156:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	0	33	theme	Mammalian	156:164	arg1	Oligomannose					166:177	Mammalian Oligomannose	156:177	Mammalian Oligomannose	156:177	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	6	34	theme	-linked	1209:1215	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	9	35	theme	Possible	1604:1611	arg1	reasons					1613:1619	Possible reasons	1604:1619	Possible reasons for this outcome	1604:1636	Possible reasons for this outcome are discussed herein along with paths for improvement.					
31010286	0	36	theme	Undecasaccharide	16:31	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.	0:206	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	1	37	theme	antigenic	308:316	arg1	oligomannoses					328:340	antigenic mammalian oligomannoses	308:340	antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	308:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	8	38	theme	oligomannose-specific	1508:1528	arg1	antibodies					1547:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	However, representative oligomannose-specific HIV-neutralizing antibodies bound the undecasaccharide conjugates poorly.					
31010286	8	39	theme	HIV-neutralizing	1530:1545	arg1	antibodies					1547:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	representative oligomannose-specific HIV-neutralizing antibodies	1493:1556	However, representative oligomannose-specific HIV-neutralizing antibodies bound the undecasaccharide conjugates poorly.					
31010286	2	40	theme	hybrid	457:462	arg1	structure					464:472	a hybrid structure	455:472	a hybrid structure of viral and bacterial epitopes	455:504	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	1	41	theme	mammalian	318:326	arg1	oligomannoses					328:340	antigenic mammalian oligomannoses	308:340	antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	308:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	0	42	theme	Neutralizing	129:140	arg1	Antibodies					142:151	Neutralizing Antibodies	129:151	Neutralizing Antibodies to Mammalian Oligomannose	129:177	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	6	43	theme	O-6	1149:1151	arg1	Glycosylation					1132:1144	Glycosylation	1132:1144	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1132:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	3	44	theme	residue	821:827	arg1	OH-5					795:798	OH-5	795:798	OH-5 of the innermost Kdo residue	795:827	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	1	45	from	Rv3	276:278	arg1	LOS					230:232	LOS	230:232	LOS	230:232	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	45	from	Rv3	276:278	arg1	Lipooligosaccharides					208:227	Lipooligosaccharides	208:227	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3	208:278	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	3	46	theme	central	714:720	arg1	donor					774:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	4	47	theme	glucosamine	890:900	arg1	units					902:906	the glucosamine units	886:906	the glucosamine units	886:906	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	7	48	theme	adipic	1426:1431	arg1	ligand					1374:1379	the 3-aminopropyl ligand	1356:1379	the 3-aminopropyl ligand	1356:1379	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	48	theme	adipic	1426:1431	arg1	ester					1452:1456	adipic acid succinimidoyl ester	1426:1456	adipic acid succinimidoyl ester	1426:1456	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	2	49	theme	HIV-neutralizing	575:590	arg1	antibodies					592:601	oligomannose-specific HIV-neutralizing antibodies	553:601	oligomannose-specific HIV-neutralizing antibodies	553:601	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	6	50	with	mannose	1178:1184	arg1	unit					1257:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1191:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	5	51	theme	D1-arm	1115:1120	arg1	fragment					1122:1129	the D1-arm fragment	1111:1129	the D1-arm fragment	1111:1129	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	2	52	theme	epitopes	497:504	arg1	structure					464:472	a hybrid structure	455:472	a hybrid structure of viral and bacterial epitopes	455:504	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	2	53	theme	Rv3	638:640	arg1	structure					646:654	the Rv3 LOS structure	634:654	the Rv3 LOS structure	634:654	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	6	54	theme	D3-arm	1310:1315	arg1	extension					1317:1325	a D3-arm extension	1308:1325	a D3-arm extension	1308:1325	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	7	55	theme	3-aminopropyl	1360:1372	arg1	ligand					1374:1379	the 3-aminopropyl ligand	1356:1379	the 3-aminopropyl ligand	1356:1379	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	55	theme	3-aminopropyl	1360:1372	arg1	ester					1452:1456	adipic acid succinimidoyl ester	1426:1456	adipic acid succinimidoyl ester	1426:1456	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	55	theme	3-aminopropyl	1360:1372	arg1	isothiocyanate					1408:1421	an isothiocyanate	1405:1421	an isothiocyanate	1405:1421	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	2	56	theme	bacterial	487:495	arg1	epitopes					497:504	viral and bacterial epitopes	477:504	viral and bacterial epitopes	477:504	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	1	57	theme	HIV-1	349:353	arg1	glycoprotein					364:375	the HIV-1 envelope glycoprotein spike	345:381	the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	345:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	57	theme	HIV-1	349:353	arg1	targets					392:398	targets	392:398	targets for broadly neutralizing antibodies	392:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	4	58	theme	imidate	966:972	arg1	byproducts					974:983	unwanted imidate byproducts	957:983	unwanted imidate byproducts	957:983	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	3	59	theme	mannose	745:751	arg1	donor					774:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	4	60	theme	units	902:906	arg1	groups					876:881	the N-acetylamino groups	858:881	the N-acetylamino groups of the glucosamine units	858:906	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	4	60	theme	units	902:906	arg1	units					902:906	the glucosamine units	886:906	the glucosamine units	886:906	To assemble larger glycans, the N-acetylamino groups of the glucosamine units were converted to imides to prevent formation of unwanted imidate byproducts.					
31010286	1	61	theme	envelope	355:362	arg1	glycoprotein					364:375	the HIV-1 envelope glycoprotein spike	345:381	the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies	345:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	1	61	theme	envelope	355:362	arg1	targets					392:398	targets	392:398	targets for broadly neutralizing antibodies	392:434	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	0	62	theme	HIV-1	186:190	arg1	Spike					201:205	the HIV-1 Envelope Spike	182:205	the HIV-1 Envelope Spike	182:205	Synthesis of an Undecasaccharide Featuring an Oligomannosidic Heptasaccharide and a Bacterial Kdo-lipid A Backbone for Eliciting Neutralizing Antibodies to Mammalian Oligomannose on the HIV-1 Envelope Spike.					
31010286	3	63	theme	trichloroacetimidate	753:772	arg1	donor					774:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	3	64	theme	Kdo2GlcNAc2	673:683	arg1	precursor					701:709	a Kdo2GlcNAc2 tetrasaccharide precursor	671:709	a Kdo2GlcNAc2 tetrasaccharide precursor	671:709	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	3	65	theme	Kdo	817:819	arg1	residue					821:827	the innermost Kdo residue	803:827	the innermost Kdo residue	803:827	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	5	66	theme	-linked	1052:1058	arg1	donor					1094:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	an α-(1→2)-linked mannotriosyl trichloroacetimidate donor	1042:1098	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	3	67	theme	tetrasaccharide	685:699	arg1	precursor					701:709	a Kdo2GlcNAc2 tetrasaccharide precursor	671:709	a Kdo2GlcNAc2 tetrasaccharide precursor	671:709	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	5	68	theme	pentasaccharide	1012:1026	arg1	acceptor					1028:1035	the pentasaccharide acceptor	1008:1035	the pentasaccharide acceptor	1008:1035	Blockwise coupling of the pentasaccharide acceptor with an α-(1→2)-linked mannotriosyl trichloroacetimidate donor introduced the D1-arm fragment.					
31010286	1	69	theme	bacterium	244:252	arg1	Rv3					276:278	the bacterium Rhizobium radiobacter Rv3	240:278	the bacterium Rhizobium radiobacter Rv3	240:278	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	2	70	theme	design	527:532	arg1	strategy					534:541	a vaccine design strategy	517:541	a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies	517:601	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	3	71	theme	protected	735:743	arg1	donor					774:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	a central orthogonally protected mannose trichloroacetimidate donor	712:778	Starting from a Kdo2GlcNAc2 tetrasaccharide precursor, a central orthogonally protected mannose trichloroacetimidate donor was coupled to OH-5 of the innermost Kdo residue.					
31010286	7	72	theme	succinimidoyl	1438:1450	arg1	ligand					1374:1379	the 3-aminopropyl ligand	1356:1379	the 3-aminopropyl ligand	1356:1379	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	72	theme	succinimidoyl	1438:1450	arg1	ester					1452:1456	adipic acid succinimidoyl ester	1426:1456	adipic acid succinimidoyl ester	1426:1456	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	73	theme	acid	1433:1436	arg1	ligand					1374:1379	the 3-aminopropyl ligand	1356:1379	the 3-aminopropyl ligand	1356:1379	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	7	73	theme	acid	1433:1436	arg1	ester					1452:1456	adipic acid succinimidoyl ester	1426:1456	adipic acid succinimidoyl ester	1426:1456	Global deprotection yielded the 3-aminopropyl ligand, which was activated as an isothiocyanate or adipic acid succinimidoyl ester and conjugated to CRM197.					
31010286	1	74	theme	Rhizobium	254:262	arg1	Rv3					276:278	the bacterium Rhizobium radiobacter Rv3	240:278	the bacterium Rhizobium radiobacter Rv3	240:278	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31010286	2	75	theme	vaccine	519:525	arg1	design					527:532	vaccine design	519:532	a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies	517:601	Here, we prepared a hybrid structure of viral and bacterial epitopes as part of a vaccine design strategy to elicit oligomannose-specific HIV-neutralizing antibodies using glycoconjugates based on the Rv3 LOS structure.					
31010286	6	76	gly	Glycosylation	1132:1144	arg1	O-6					1149:1151	O-6	1149:1151	O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit	1149:1260	Glycosylation of O-6 of the central branching mannose with an α-(1→2)-α-(1→6)-linked mannotriosyl trichloroacetimidate donor unit then furnished the undecasaccharide harboring a D3-arm extension.					
31010286	1	77	theme	radiobacter	264:274	arg1	Rv3					276:278	the bacterium Rhizobium radiobacter Rv3	240:278	the bacterium Rhizobium radiobacter Rv3	240:278	Lipooligosaccharides (LOS) from the bacterium Rhizobium radiobacter Rv3 are structurally related to antigenic mammalian oligomannoses on the HIV-1 envelope glycoprotein spike that are targets for broadly neutralizing antibodies.					
31220627	7	0	dep	technological	1179:1191	arg1	uses					1193:1196	uses	1193:1196	uses	1193:1196	These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.					
31220627	1	1	theme	Brazilian	152:160	arg1	Cerrado					162:168	the Brazilian Cerrado	148:168	the Brazilian Cerrado	148:168	Guavira is a plant that belongs to Myrtaceae family, being widespread in the Brazilian Cerrado.					
31220627	7	2	theme	future	1154:1159	arg1	nutraceutical					1161:1173	future nutraceutical	1154:1173	future nutraceutical	1154:1173	These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.					
31220627	3	3	theme	arabinogalactans	556:571	arg1	composition					527:537	the composition	523:537	the composition of related plant arabinogalactans and hemicelluloses present in the literature	523:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	6	4	theme	α-D-GlcpA/4-O-methyl-α-D-GlcpA	936:965	arg1	group					967:971	α-D-GlcpA/4-O-methyl-α-D-GlcpA group	936:971	α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan	936:1008	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	6	5	attach	linked	973:978	arg1	O-2					983:985	O-2	983:985	O-2 of a (1 → 4)-β-D-xylan	983:1008	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	6	5	attach	linked	973:978	arg2	group					967:971	α-D-GlcpA/4-O-methyl-α-D-GlcpA group	936:971	α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan	936:1008	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	1	6	from	widespread	134:143	arg1	Cerrado					162:168	the Brazilian Cerrado	148:168	the Brazilian Cerrado	148:168	Guavira is a plant that belongs to Myrtaceae family, being widespread in the Brazilian Cerrado.					
31220627	2	7	theme	pulp	278:281	arg1	production					283:292	pulp production	278:292	pulp production	278:292	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	6	8	contain	contained	903:911	arg1	fraction					894:901	Hemicellulosic fraction	879:901	Hemicellulosic fraction	879:901	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	6	8	contain	contained	903:911	arg2	glucuronoxylan					915:928	a glucuronoxylan	913:928	a glucuronoxylan	913:928	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	1	9	theme	Myrtaceae	110:118	arg1	family					120:125	Myrtaceae family	110:125	Myrtaceae family	110:125	Guavira is a plant that belongs to Myrtaceae family, being widespread in the Brazilian Cerrado.					
31220627	6	10	theme	uronic	1026:1031	arg1	residue					1038:1044	one uronic acid residue	1022:1044	one uronic acid residue for every six xylose units	1022:1071	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	3	11	theme	related	542:548	arg1	arabinogalactans					556:571	related plant arabinogalactans	542:571	related plant arabinogalactans	542:571	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	5	12	theme	terminal	791:798	arg1	units					809:813	terminal β-L-Araf units	791:813	terminal β-L-Araf units	791:813	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	5	13	theme	NMR	732:734	arg1	analyses					736:743	Mono and bidimensional NMR analyses	709:743	analyses	736:743	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	5	14	theme	Mono	709:712	arg1	analyses					736:743	Mono and bidimensional NMR analyses	709:743	analyses	736:743	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	3	15	theme	plant	550:554	arg1	arabinogalactans					556:571	related plant arabinogalactans	542:571	related plant arabinogalactans	542:571	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	16	attach	present	592:598	arg1	literature					607:616	the literature	603:616	the literature	603:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	16	attach	present	592:598	arg2	arabinogalactans					556:571	related plant arabinogalactans	542:571	related plant arabinogalactans	542:571	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	5	17	theme	β-L-Araf	800:807	arg1	units					809:813	terminal β-L-Araf units	791:813	terminal β-L-Araf units	791:813	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	6	18	theme	acid	1033:1036	arg1	residue					1038:1044	one uronic acid residue	1022:1044	one uronic acid residue for every six xylose units	1022:1071	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	5	19	theme	units	809:813	arg1	presence					779:786	the presence	775:786	the presence	775:786	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	3	20	from	literature	607:616	arg1	present					592:598	present	592:598	present	592:598	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	2	21	theme	NMR	382:384	arg1	experiments					386:396	NMR experiments	382:396	NMR experiments	382:396	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	7	22	theme	pomace	1103:1108	arg1	polysaccharides					1110:1124	guavira pomace polysaccharides	1095:1124	guavira pomace polysaccharides	1095:1124	These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.					
31220627	3	23	from	data	515:518	arg1	composition					527:537	the composition	523:537	the composition of related plant arabinogalactans and hemicelluloses present in the literature	523:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	24	theme	hemicelluloses	577:590	arg1	composition					527:537	the composition	523:537	the composition of related plant arabinogalactans and hemicelluloses present in the literature	523:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	2	25	from	pomace	241:246	arg1	polysaccharides					212:226	pectic and hemicellulosic polysaccharides	186:226	pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production,	186:293	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	6	26	theme	-β-D-xylan	999:1008	arg1	O-2					983:985	O-2	983:985	O-2 of a (1 → 4)-β-D-xylan	983:1008	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	5	27	theme	arabinan	753:760	arg1	analyses					736:743	Mono and bidimensional NMR analyses	709:743	analyses	736:743	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	6	28	dep	-β-D-xylan	999:1008	arg1	1 → 4					993:997	1 → 4	993:997	1 → 4	993:997	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	3	29	theme	present	592:598	arg1	arabinogalactans					556:571	related plant arabinogalactans	542:571	related plant arabinogalactans	542:571	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	0	30	theme	guavira	53:59	arg1	pomace					67:72	guavira fruit pomace	53:72	guavira fruit pomace	53:72	β-L-Araf-containing arabinan and glucuronoxylan from guavira fruit pomace.					
31220627	2	31	theme	guavira	233:239	arg1	residue					265:271	an agroindustry residue	249:271	an agroindustry residue from pulp production	249:292	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	2	31	theme	guavira	233:239	arg1	pomace					241:246	guavira pomace	233:246	guavira pomace	233:246	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	3	32	theme	uronic	464:469	arg1	acids					471:475	uronic acids	464:475	uronic acids	464:475	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	33	theme	monosaccharides	430:444	arg1	nature					454:459	the nature	450:459	the nature of uronic acids	450:475	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	33	theme	monosaccharides	430:444	arg1	configurations					412:425	The absolute configurations	399:425	The absolute configurations of monosaccharides	399:444	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	7	34	theme	guavira	1095:1101	arg1	polysaccharides					1110:1124	guavira pomace polysaccharides	1095:1124	guavira pomace polysaccharides	1095:1124	These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.					
31220627	3	35	theme	acids	471:475	arg1	nature					454:459	the nature	450:459	the nature of uronic acids	450:475	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	35	theme	acids	471:475	arg1	configurations					412:425	The absolute configurations	399:425	The absolute configurations of monosaccharides	399:444	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	3	36	theme	absolute	403:410	arg1	configurations					412:425	The absolute configurations	399:425	The absolute configurations of monosaccharides	399:444	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	6	37	theme	Hemicellulosic	879:892	arg1	fraction					894:901	Hemicellulosic fraction	879:901	Hemicellulosic fraction	879:901	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	2	38	theme	monosaccharide	338:351	arg1	composition					353:363	monosaccharide composition	338:363	monosaccharide composition	338:363	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	3	39	theme	numerous	506:513	arg1	data					515:518	numerous data	506:518	numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature	506:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	0	40	theme	fruit	61:65	arg1	pomace					67:72	guavira fruit pomace	53:72	guavira fruit pomace	53:72	β-L-Araf-containing arabinan and glucuronoxylan from guavira fruit pomace.					
31220627	7	41	theme	industrial	1207:1216	arg1	waste					1218:1222	this industrial waste	1202:1222	this industrial waste	1202:1222	These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.					
31220627	2	42	theme	hemicellulosic	197:210	arg1	polysaccharides					212:226	pectic and hemicellulosic polysaccharides	186:226	pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production,	186:293	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	2	43	theme	pectic	186:191	arg1	polysaccharides					212:226	pectic and hemicellulosic polysaccharides	186:226	pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production,	186:293	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	2	44	theme	agroindustry	252:263	arg1	residue					265:271	an agroindustry residue	249:271	an agroindustry residue from pulp production	249:292	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	2	44	theme	agroindustry	252:263	arg1	pomace					241:246	guavira pomace	233:246	guavira pomace	233:246	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	6	45	theme	xylose	1060:1065	arg1	units					1067:1071	every six xylose units	1050:1071	every six xylose units	1050:1071	Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1 → 4)-β-D-xylan, presenting one uronic acid residue for every six xylose units.					
31220627	3	46	from	present	592:598	arg1	literature					607:616	the literature	603:616	the literature	603:616	The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature.					
31220627	2	47	from	production	283:292	arg1	residue					265:271	an agroindustry residue	249:271	an agroindustry residue from pulp production	249:292	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	2	47	from	production	283:292	arg1	pomace					241:246	guavira pomace	233:246	guavira pomace	233:246	In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments.					
31220627	5	48	theme	bidimensional	718:730	arg1	analyses					736:743	Mono and bidimensional NMR analyses	709:743	analyses	736:743	Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal β-L-Araf units, whose occurrence has been scarcely reported in the literature.					
31220627	1	49	from	Cerrado	162:168	arg1	widespread					134:143	widespread	134:143	widespread	134:143	Guavira is a plant that belongs to Myrtaceae family, being widespread in the Brazilian Cerrado.					
30842877	2	0	from	metastases	359:368	arg1	abundant					341:348	abundant	341:348	abundant	341:348	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	2	0	from	metastases	359:368	arg1	IgA1					303:306	IgA1	303:306	IgA1	303:306	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	8	1	from	analysis	1430:1437	arg1	BCa					1470:1472	BCa	1470:1472	BCa	1470:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	7	2	theme	Immunohistochemical	1300:1318	arg1	analysis					1320:1327	Immunohistochemical analysis	1300:1327	Immunohistochemical analysis of tumour tissue samples	1300:1352	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	8	3	theme	IgA1	1515:1518	arg1	glycosylation					1520:1532	IgA1 glycosylation	1515:1532	IgA1 glycosylation	1515:1532	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	3	4	from	patients	451:458	arg1	samples					438:444	serum samples	432:444	serum samples from patients with breast cancer (BCa)	432:483	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	7	5	theme	samples	1346:1352	arg1	analysis					1320:1327	Immunohistochemical analysis	1300:1327	Immunohistochemical analysis of tumour tissue samples	1300:1352	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	5	6	dep	Sambucus	952:959	arg1	nigra					961:965	nigra	961:965	nigra	961:965	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	8	7	from	glycosylation	1453:1465	arg1	BCa					1470:1472	BCa	1470:1472	BCa	1470:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	1	8	theme	solid	157:161	arg1	cancers					163:169	solid cancers	157:169	solid cancers	157:169	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	7	9	theme	tumour	1332:1337	arg1	samples					1346:1352	tumour tissue samples	1332:1352	tumour tissue samples	1332:1352	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	4	10	theme	asialo-Thomsen-Friedenreich	764:790	arg1	TF					801:802	TF	801:802	TF	801:802	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	10	theme	asialo-Thomsen-Friedenreich	764:790	arg1	antigen					792:798	asialo-Thomsen-Friedenreich antigen	764:798	asialo-Thomsen-Friedenreich antigen (TF)	764:803	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	0	11	link	α2,6-linked	37:47	arg1	acid					56:59	α2,6-linked sialic acid	37:59	α2,6-linked sialic acid in breast cancer	37:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	6	12	theme	HPA	1089:1091	arg1	binding					1093:1099	HPA binding	1089:1099	HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively	1089:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	6	13	theme	distant	1169:1175	arg1	metastases					1177:1186	distant metastases	1169:1186	distant metastases (χ 2 13.359; n = 114; p = 0.020)	1169:1219	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	2	14	theme	many	276:279	arg1	proteins					293:300	the many HPA binding proteins	272:300	the many HPA binding proteins	272:300	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	4	15	theme	O-linked	836:843	arg1	preparations					852:863	the O-linked glycan preparations	832:863	the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals	832:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	16	theme	Detailed	552:559	arg1	analysis					591:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	5	17	from	increase	940:947	arg1	binding					967:973	Sambucus nigra binding	952:973	Sambucus nigra binding	952:973	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	4	18	theme	spectrometric	566:578	arg1	analysis					591:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	8	19	from	BCa	1470:1472	arg1	analysis					1430:1437	a detailed analysis	1419:1437	a detailed analysis of serum IgA1 glycosylation in BCa	1419:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	5	20	theme	increased	1003:1011	arg1	acid					1032:1035	increased α2,6-linked sialic acid	1003:1035	increased α2,6-linked sialic acid on IgA1 in BCa	1003:1050	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	1	21	theme	HPA	197:199	arg1	partners					209:216	HPA binding partners	197:216	HPA binding partners in tumour tissue and serum	197:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	4	22	theme	disialo-TF	809:818	arg1	antigens					820:827	disialo-TF antigens	809:827	disialo-TF antigens	809:827	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	0	23	from	acid	56:59	arg1	cancer					71:76	breast cancer	64:76	breast cancer	64:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	6	24	theme	independent	1143:1153	arg1	predictors					1155:1164	significant independent predictors	1131:1164	significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020)	1131:1219	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	3	25	theme	IgA1	504:507	arg1	glycosylation					509:521	IgA1 glycosylation	504:521	IgA1 glycosylation	504:521	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	8	26	gly	glycosylation	1453:1465	arg1	BCa					1470:1472	BCa	1470:1472	BCa	1470:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	1	27	from	partners	209:216	arg1	serum					239:243	serum	239:243	serum	239:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	27	from	partners	209:216	arg1	tissue					228:233	tumour tissue	221:233	tumour tissue	221:233	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	0	28	theme	acid	56:59	arg1	levels					27:32	increased levels	17:32	increased levels of α2,6-linked sialic acid in breast cancer	17:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	6	29	with	predictors	1155:1164	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	4	30	theme	healthy	908:914	arg1	individuals					924:934	healthy control individuals	908:934	healthy control individuals	908:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	31	theme	N-linked	642:649	arg1	glycans					651:657	disialo-biantennary N-linked glycans	622:657	disialo-biantennary N-linked glycans	622:657	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	0	32	theme	breast	64:69	arg1	cancer					71:76	breast cancer	64:76	breast cancer	64:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	4	33	dep	<	688:688	arg1	p					720:720	p	720:720	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	33	dep	<	688:688	arg1	fucosylated					707:717	non-core fucosylated	698:717	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	33	dep	<	688:688	arg1	fucosylated					737:747	core fucosylated	732:747	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	34	theme	negative	1239:1246	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	5	35	theme	sialic	1025:1030	arg1	acid					1032:1035	increased α2,6-linked sialic acid	1003:1035	increased α2,6-linked sialic acid on IgA1 in BCa	1003:1050	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	4	36	theme	cancer	878:883	arg1	patients					885:892	cancer patients	878:892	cancer patients compared with healthy control individuals	878:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	37	theme	non-core	698:705	arg1	fucosylated					707:717	non-core fucosylated	698:717	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	38	theme	positive	1226:1233	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	0	39	from	levels	27:32	arg1	cancer					71:76	breast cancer	64:76	breast cancer	64:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	0	40	theme	Serum	0:4	arg1	IgA1					6:9	Serum IgA1	0:9	Serum IgA1	0:9	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	4	41	from	patients	676:683	arg1	IgA1					662:665	IgA1	662:665	IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated)	662:748	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	42	theme	p	686:686	arg1	<					688:688	p < 0.0001	686:695	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	5	43	from	acid	1032:1035	arg1	BCa					1048:1050	BCa	1048:1050	BCa	1048:1050	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	5	43	from	acid	1032:1035	arg1	IgA1					1040:1043	IgA1	1040:1043	IgA1	1040:1043	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	1	44	theme	Helix	90:94	arg1	HPA					116:118	HPA	116:118	HPA	116:118	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	44	theme	Helix	90:94	arg1	agglutinin					104:113	The lectin Helix pomatia agglutinin	79:113	The lectin Helix pomatia agglutinin (HPA)	79:119	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	2	45	theme	liver	353:357	arg1	metastases					359:368	liver metastases	353:368	liver metastases	353:368	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	6	46	theme	%	1283:1283	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	8	47	theme	serum	1442:1446	arg1	glycosylation					1453:1465	serum IgA1 glycosylation	1442:1465	serum IgA1 glycosylation in BCa	1442:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	3	48	gly	glycosylation	390:402	arg1	study					379:383	this study	374:383	this study	374:383	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	3	48	gly	glycosylation	390:402	arg1	IgA1					407:410	IgA1	407:410	IgA1	407:410	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	0	49	theme	α2,6-linked	37:47	arg1	acid					56:59	α2,6-linked sialic acid	37:59	α2,6-linked sialic acid in breast cancer	37:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	7	50	theme	BCa	1386:1388	arg1	tissue					1390:1395	BCa tissue	1386:1395	BCa tissue	1386:1395	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	2	51	from	abundant	341:348	arg1	metastases					359:368	liver metastases	353:368	liver metastases	353:368	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	8	52	theme	glycosylation	1453:1465	arg1	analysis					1430:1437	a detailed analysis	1419:1437	a detailed analysis of serum IgA1 glycosylation in BCa	1419:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	8	53	theme	glycosylation	1520:1532	arg1	utility					1504:1510	the potential utility	1490:1510	the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication	1490:1571	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	1	54	theme	binding	201:207	arg1	partners					209:216	HPA binding partners	197:216	HPA binding partners in tumour tissue and serum	197:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	4	55	theme	=	722:722	arg1	p					720:720	p	720:720	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	56	link	O-linked	836:843	arg1	preparations					852:863	the O-linked glycan preparations	832:863	the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals	832:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	7	57	from	tissue	1390:1395	arg1	detectable					1372:1381	detectable	1372:1381	detectable	1372:1381	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	8	58	theme	potential	1494:1502	arg1	utility					1504:1510	the potential utility	1490:1510	the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication	1490:1571	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	3	59	with	patients	451:458	arg1	BCa					480:482	BCa	480:482	BCa	480:482	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	3	59	with	patients	451:458	arg1	cancer					472:477	breast cancer	465:477	breast cancer (BCa)	465:483	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	4	60	from	increase	610:617	arg1	glycans					651:657	disialo-biantennary N-linked glycans	622:657	disialo-biantennary N-linked glycans	622:657	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	61	from	IgA1	870:873	arg1	preparations					852:863	the O-linked glycan preparations	832:863	the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals	832:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	62	link	N-linked	642:649	arg1	glycans					651:657	disialo-biantennary N-linked glycans	622:657	disialo-biantennary N-linked glycans	622:657	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	7	63	theme	tissue	1339:1344	arg1	samples					1346:1352	tumour tissue samples	1332:1352	tumour tissue samples	1332:1352	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	6	64	theme	χ	1189:1189	arg1	13.359					1193:1198	χ 2 13.359	1189:1198	χ 2 13.359	1189:1198	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	3	65	theme	breast	465:470	arg1	BCa					480:482	BCa	480:482	BCa	480:482	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	3	65	theme	breast	465:470	arg1	cancer					472:477	breast cancer	465:477	breast cancer (BCa)	465:483	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	4	66	dep	=	722:722	arg1	0.0345					724:729	0.0345	724:729	0.0345	724:729	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	67	theme	structural	580:589	arg1	analysis					591:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	68	theme	metastases	1177:1186	arg1	predictors					1155:1164	significant independent predictors	1131:1164	significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020)	1131:1219	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	2	69	theme	HPA	281:283	arg1	proteins					293:300	the many HPA binding proteins	272:300	the many HPA binding proteins	272:300	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	6	70	dep	=	1203:1203	arg1	13.359					1193:1198	χ 2 13.359	1189:1198	χ 2 13.359	1189:1198	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	1	71	from	serum	239:243	arg1	aim					261:263	an important aim	248:263	an important aim	248:263	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	71	from	serum	239:243	arg1	identification					179:192	the identification	175:192	the identification of HPA binding partners in tumour tissue and serum	175:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	0	72	from	cancer	71:76	arg1	levels					27:32	increased levels	17:32	increased levels of α2,6-linked sialic acid in breast cancer	17:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	6	73	theme	regression	1062:1071	arg1	analysis					1073:1080	Logistic regression analysis	1053:1080	Logistic regression analysis	1053:1080	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	1	74	from	tissue	228:233	arg1	aim					261:263	an important aim	248:263	an important aim	248:263	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	74	from	tissue	228:233	arg1	identification					179:192	the identification	175:192	the identification of HPA binding partners in tumour tissue and serum	175:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	3	75	theme	glycosylation	509:521	arg1	utility					493:499	the utility	489:499	the utility of IgA1 glycosylation as a biomarker	489:536	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	4	76	theme	glycan	845:850	arg1	preparations					852:863	the O-linked glycan preparations	832:863	the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals	832:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	4	77	theme	mass	561:564	arg1	analysis					591:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis	552:598	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	5	78	theme	α2,6-linked	1013:1023	arg1	acid					1032:1035	increased α2,6-linked sialic acid	1003:1035	increased α2,6-linked sialic acid on IgA1 in BCa	1003:1050	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	1	79	theme	pomatia	96:102	arg1	HPA					116:118	HPA	116:118	HPA	116:118	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	79	theme	pomatia	96:102	arg1	agglutinin					104:113	The lectin Helix pomatia agglutinin	79:113	The lectin Helix pomatia agglutinin (HPA)	79:119	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	6	80	theme	significant	1131:1141	arg1	predictors					1155:1164	significant independent predictors	1131:1164	significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020)	1131:1219	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	1	81	gly	glycosylation	140:152	arg1	cancers					163:169	solid cancers	157:169	solid cancers	157:169	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	82	from	identification	179:192	arg1	serum					239:243	serum	239:243	serum	239:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	82	from	identification	179:192	arg1	tissue					228:233	tumour tissue	221:233	tumour tissue	221:233	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	4	83	dep	patients	676:683	arg1	<					688:688	p < 0.0001	686:695	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	84	theme	%	1273:1273	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	4	85	theme	patients	885:892	arg1	IgA1					870:873	IgA1	870:873	IgA1 of cancer patients compared with healthy control individuals	870:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	86	theme	tumour	1113:1118	arg1	size					1120:1123	tumour size	1113:1123	tumour size	1113:1123	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	1	87	theme	altered	132:138	arg1	glycosylation					140:152	altered glycosylation	132:152	altered glycosylation in solid cancers	132:169	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	5	88	link	α2,6-linked	1013:1023	arg1	acid					1032:1035	increased α2,6-linked sialic acid	1003:1035	increased α2,6-linked sialic acid on IgA1 in BCa	1003:1050	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	4	89	theme	disialo-biantennary	622:640	arg1	glycans					651:657	disialo-biantennary N-linked glycans	622:657	disialo-biantennary N-linked glycans	622:657	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	6	90	theme	predictive	1248:1257	arg1	values					1259:1264	positive and negative predictive values	1226:1264	positive and negative predictive values of 65.7% and 64.6%, respectively	1226:1297	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	1	91	from	glycosylation	140:152	arg1	cancers					163:169	solid cancers	157:169	solid cancers	157:169	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	5	92	theme	acid	1032:1035	arg1	suggestive					989:998	suggestive	989:998	suggestive	989:998	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	7	93	from	detectable	1372:1381	arg1	tissue					1390:1395	BCa tissue	1386:1395	BCa tissue	1386:1395	Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue.					
30842877	8	94	theme	detailed	1421:1428	arg1	analysis					1430:1437	a detailed analysis	1419:1437	a detailed analysis of serum IgA1 glycosylation in BCa	1419:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	4	95	theme	BCa	672:674	arg1	patients					676:683	BCa patients	672:683	BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated)	672:748	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	0	96	theme	increased	17:25	arg1	levels					27:32	increased levels	17:32	increased levels of α2,6-linked sialic acid in breast cancer	17:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	1	97	theme	important	251:259	arg1	aim					261:263	an important aim	248:263	an important aim	248:263	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	97	theme	important	251:259	arg1	identification					179:192	the identification	175:192	the identification of HPA binding partners in tumour tissue and serum	175:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	98	theme	lectin	83:88	arg1	HPA					116:118	HPA	116:118	HPA	116:118	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	98	theme	lectin	83:88	arg1	agglutinin					104:113	The lectin Helix pomatia agglutinin	79:113	The lectin Helix pomatia agglutinin (HPA)	79:119	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	3	99	theme	IgA1	407:410	arg1	glycosylation					390:402	the glycosylation	386:402	the glycosylation of IgA1	386:410	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	4	100	theme	control	916:922	arg1	individuals					924:934	healthy control individuals	908:934	healthy control individuals	908:934	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	8	101	theme	IgA1	1448:1451	arg1	glycosylation					1453:1465	serum IgA1 glycosylation	1442:1465	serum IgA1 glycosylation in BCa	1442:1472	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	0	102	theme	sialic	49:54	arg1	acid					56:59	α2,6-linked sialic acid	37:59	α2,6-linked sialic acid in breast cancer	37:76	Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.					
30842877	5	103	theme	Sambucus	952:959	arg1	binding					967:973	Sambucus nigra binding	952:973	Sambucus nigra binding	952:973	An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa.					
30842877	6	104	theme	Logistic	1053:1060	arg1	analysis					1073:1080	Logistic regression analysis	1053:1080	Logistic regression analysis	1053:1080	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	4	105	theme	core	732:735	arg1	fucosylated					737:747	core fucosylated	732:747	p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated	686:747	Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals.					
30842877	3	106	theme	serum	432:436	arg1	samples					438:444	serum samples	432:444	serum samples from patients with breast cancer (BCa)	432:483	In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed.					
30842877	2	107	theme	binding	285:291	arg1	proteins					293:300	the many HPA binding proteins	272:300	the many HPA binding proteins	272:300	Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases.					
30842877	1	108	theme	partners	209:216	arg1	aim					261:263	an important aim	248:263	an important aim	248:263	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	1	108	theme	partners	209:216	arg1	identification					179:192	the identification	175:192	the identification of HPA binding partners in tumour tissue and serum	175:243	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30842877	6	109	dep	metastases	1177:1186	arg1	=					1203:1203	=	1203:1203	=	1203:1203	Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ 2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively.					
30842877	8	110	theme	BCa	1553:1555	arg1	prognostication					1557:1571	BCa prognostication	1553:1571	BCa prognostication	1553:1571	This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.					
30842877	1	111	theme	tumour	221:226	arg1	tissue					228:233	tumour tissue	221:233	tumour tissue	221:233	The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim.					
30553311	2	0	used	used	296:299	arg2	yield					286:290	Polysaccharide yield	271:290	Polysaccharide yield	271:290	Polysaccharide yield was used as index to evaluate the extraction process.					
30553311	2	0	used	used	296:299	arg2	index					304:308	index	304:308	index	304:308	Polysaccharide yield was used as index to evaluate the extraction process.					
30553311	9	1	theme	total	1150:1154	arg1	carbohydrates					1156:1168	total carbohydrates	1150:1168	total carbohydrates	1150:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	11	2	theme	cells	1535:1539	arg1	apoptosis					1507:1515	apoptosis	1507:1515	apoptosis of SGC-7901 tumor cells in vitro	1507:1548	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	11	2	theme	cells	1535:1539	arg1	effect					1564:1569	antitumor effect	1554:1569	antitumor effect on SGC-7901 tumor-bearing mice	1554:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	7	3	theme	optimal	821:827	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions for MAE of PHP	817:853	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	3	theme	optimal	821:827	arg1	ratio					860:864	ratio	860:864	ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min	860:956	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	5	4	theme	PHP	616:618	arg1	characterization					596:611	Chemical characterization	587:611	Chemical characterization of PHP	587:618	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	10	5	theme	Thermal	1364:1370	arg1	analysis					1384:1391	Thermal gravimetric analysis	1364:1391	Thermal gravimetric analysis	1364:1391	Thermal gravimetric analysis suggested that PHP was relatively stable below 170℃.					
30553311	1	6	theme	extract	203:209	arg1	polysaccharides					211:225	extract polysaccharides	203:225	extract polysaccharides from Porphyra Haitanensis (PHP) with water	203:268	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	9	7	theme	uronic	1188:1193	arg1	residue					1200:1206	uronic acid residue	1188:1206	uronic acid residue	1188:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	0	8	from	activities	87:96	arg1	haitanensis					131:141	porphyra haitanensis	122:141	porphyra haitanensis	122:141	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	9	theme	residue	1200:1206	arg1	residue					1200:1206	uronic acid residue	1188:1206	uronic acid residue	1188:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	9	theme	residue	1200:1206	arg1	proteins					1215:1222	no proteins	1212:1222	no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1212:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	9	theme	residue	1200:1206	arg1	%					1145:1145	75.36 ± 1.48%	1133:1145	75.36 ± 1.48% of total carbohydrates	1133:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	9	theme	residue	1200:1206	arg1	carbohydrates					1156:1168	total carbohydrates	1150:1168	total carbohydrates	1150:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	9	theme	residue	1200:1206	arg1	%					1183:1183	24.63 ± 1.69%	1171:1183	24.63 ± 1.69% of uronic acid residue	1171:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	11	10	from	effect	1564:1569	arg1	mice					1597:1600	SGC-7901 tumor-bearing mice	1574:1600	SGC-7901 tumor-bearing mice	1574:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	1	11	theme	Microwave	144:152	arg1	technology					180:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	11	12	theme	obvious	1454:1460	arg1	effect					1462:1467	obvious effect	1454:1467	obvious effect	1454:1467	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	5	13	theme	compositions	667:678	arg1	analysis					646:653	analysis	646:653	analysis of chemical compositions, monosaccharide and thermal gravimetric	646:718	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	3	14	from	Effects	346:352	arg1	yield					458:462	polysaccharide yield	443:462	polysaccharide yield	443:462	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	2	15	theme	Polysaccharide	271:284	arg1	index					304:308	index	304:308	index	304:308	Polysaccharide yield was used as index to evaluate the extraction process.					
30553311	2	15	theme	Polysaccharide	271:284	arg1	yield					286:290	Polysaccharide yield	271:290	Polysaccharide yield	271:290	Polysaccharide yield was used as index to evaluate the extraction process.					
30553311	7	16	theme	extraction	932:941	arg1	material					882:889	raw material 28.98	878:895	raw material 28.98(mL/g)	878:901	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	16	theme	extraction	932:941	arg1	14.14 min					948:956	extraction time 14.14 min	932:956	extraction time 14.14 min	932:956	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	1	17	theme	extraction	163:172	arg1	technology					180:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	12	18	theme	cancer	1680:1685	arg1	prevention					1687:1696	cancer prevention	1680:1696	cancer prevention	1680:1696	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	1	19	used	used	195:198	arg2	technology					180:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	1	20	theme	MAE	175:177	arg1	technology					180:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	0	21	theme	activities	87:96	arg1	Optimization					0:11	Optimization	0:11	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.	0:142	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	7	22	theme	microwave	904:912	arg1	77.84 W					920:926	microwave power 77.84 W	904:926	microwave power 77.84 W	904:926	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	22	theme	microwave	904:912	arg1	material					882:889	raw material 28.98	878:895	raw material 28.98(mL/g)	878:901	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	3	23	theme	water	366:370	arg1	ratio					357:361	ratio	357:361	ratio of water to raw material (X1), microwave power (X2) and extraction time (X3)	357:438	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	0	24	theme	polysaccharides	101:115	arg1	activities					87:96	antitumor activities	77:96	antitumor activities	77:96	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	0	24	theme	polysaccharides	101:115	arg1	extraction					35:44	microwave assisted extraction	16:44	microwave assisted extraction	16:44	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	0	24	theme	polysaccharides	101:115	arg1	characterization					56:71	chemical characterization	47:71	chemical characterization	47:71	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	25	from	galactose	1304:1312	arg1	ratio					1325:1329	a molar ratio	1317:1329	a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1317:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	3	26	theme	ratio	357:361	arg1	Effects					346:352	Effects	346:352	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield	346:462	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	0	27	from	extraction	35:44	arg1	haitanensis					131:141	porphyra haitanensis	122:141	porphyra haitanensis	122:141	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	28	with	proteins	1215:1222	arg1	rhamnose					1253:1260	rhamnose	1253:1260	rhamnose	1253:1260	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	28	with	proteins	1215:1222	arg1	monosaccharides					1229:1243	monosaccharides	1229:1243	monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1229:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	29	with	%	1183:1183	arg1	rhamnose					1253:1260	rhamnose	1253:1260	rhamnose	1253:1260	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	29	with	%	1183:1183	arg1	monosaccharides					1229:1243	monosaccharides	1229:1243	monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1229:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	8	30	theme	PHP	1006:1008	arg1	extraction					1010:1019	PHP extraction	1006:1019	PHP extraction	1006:1019	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	3	31	theme	raw	375:377	arg1	X1					389:390	X1	389:390	X1	389:390	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	3	31	theme	raw	375:377	arg1	material					379:386	raw material	375:386	raw material (X1)	375:391	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	0	32	theme	microwave	16:24	arg1	extraction					35:44	microwave assisted extraction	16:44	microwave assisted extraction	16:44	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	3	33	theme	microwave	394:402	arg1	X2					411:412	X2	411:412	X2	411:412	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	3	33	theme	microwave	394:402	arg1	power					404:408	microwave power	394:408	microwave power (X2)	394:413	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	3	34	theme	extraction	419:428	arg1	time					430:433	extraction time	419:433	extraction time (X3)	419:438	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	3	34	theme	extraction	419:428	arg1	X3					436:437	X3	436:437	X3	436:437	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	0	35	theme	extraction	35:44	arg1	Optimization					0:11	Optimization	0:11	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.	0:142	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	8	36	theme	traditional	1035:1045	arg1	extraction					1057:1066	traditional hot water extraction	1035:1066	traditional hot water extraction	1035:1066	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	6	37	theme	PHP	749:751	arg1	activities					735:744	The antitumor activities	721:744	The antitumor activities of PHP in vivo and in vitro	721:772	The antitumor activities of PHP in vivo and in vitro were evaluated.					
30553311	3	38	theme	polysaccharide	443:456	arg1	yield					458:462	polysaccharide yield	443:462	polysaccharide yield	443:462	Effects of ratio of water to raw material (X1), microwave power (X2) and extraction time (X3) on polysaccharide yield were investigated.					
30553311	0	39	theme	chemical	47:54	arg1	characterization					56:71	chemical characterization	47:71	chemical characterization	47:71	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	40	with	%	1145:1145	arg1	rhamnose					1253:1260	rhamnose	1253:1260	rhamnose	1253:1260	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	40	with	%	1145:1145	arg1	monosaccharides					1229:1243	monosaccharides	1229:1243	monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1229:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	8	41	theme	water	1051:1055	arg1	extraction					1057:1066	traditional hot water extraction	1035:1066	traditional hot water extraction	1035:1066	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	4	42	theme	factor	499:504	arg1	experiment					506:515	single factor experiment	492:515	single factor experiment	492:515	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	7	43	theme	raw	878:880	arg1	material					882:889	raw material 28.98	878:895	raw material 28.98(mL/g)	878:901	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	43	theme	raw	878:880	arg1	mL/g					897:900	mL/g	897:900	mL/g	897:900	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	43	theme	raw	878:880	arg1	77.84 W					920:926	microwave power 77.84 W	904:926	microwave power 77.84 W	904:926	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	43	theme	raw	878:880	arg1	14.14 min					948:956	extraction time 14.14 min	932:956	extraction time 14.14 min	932:956	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	9	44	theme	molar	1319:1323	arg1	ratio					1325:1329	a molar ratio	1317:1329	a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1317:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	4	45	theme	response	557:564	arg1	methodology					574:584	response surface methodology	557:584	response surface methodology	557:584	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	7	46	theme	water	869:873	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions for MAE of PHP	817:853	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	46	theme	water	869:873	arg1	ratio					860:864	ratio	860:864	ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min	860:956	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	11	47	theme	SGC-7901	1574:1581	arg1	mice					1597:1600	SGC-7901 tumor-bearing mice	1574:1600	SGC-7901 tumor-bearing mice	1574:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	4	48	theme	PHP	533:535	arg1	process					522:528	MAE process	518:528	MAE process of PHP	518:535	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	7	49	theme	PHP	851:853	arg1	MAE					844:846	MAE	844:846	MAE of PHP	844:853	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	9	50	theme	chemical	1081:1088	arg1	characterization					1090:1105	chemical characterization	1081:1105	chemical characterization	1081:1105	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	5	51	theme	Chemical	587:594	arg1	characterization					596:611	Chemical characterization	587:611	Chemical characterization of PHP	587:618	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	9	52	theme	carbohydrates	1156:1168	arg1	residue					1200:1206	uronic acid residue	1188:1206	uronic acid residue	1188:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	52	theme	carbohydrates	1156:1168	arg1	proteins					1215:1222	no proteins	1212:1222	no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1212:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	52	theme	carbohydrates	1156:1168	arg1	%					1145:1145	75.36 ± 1.48%	1133:1145	75.36 ± 1.48% of total carbohydrates	1133:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	52	theme	carbohydrates	1156:1168	arg1	carbohydrates					1156:1168	total carbohydrates	1150:1168	total carbohydrates	1150:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	52	theme	carbohydrates	1156:1168	arg1	%					1183:1183	24.63 ± 1.69%	1171:1183	24.63 ± 1.69% of uronic acid residue	1171:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	10	53	theme	gravimetric	1372:1382	arg1	analysis					1384:1391	Thermal gravimetric analysis	1364:1391	Thermal gravimetric analysis	1364:1391	Thermal gravimetric analysis suggested that PHP was relatively stable below 170℃.					
30553311	2	54	theme	extraction	326:335	arg1	process					337:343	the extraction process	322:343	the extraction process	322:343	Polysaccharide yield was used as index to evaluate the extraction process.					
30553311	11	55	theme	SGC-7901	1520:1527	arg1	cells					1535:1539	SGC-7901 tumor cells	1520:1539	SGC-7901 tumor cells	1520:1539	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	11	56	theme	tumor	1529:1533	arg1	cells					1535:1539	SGC-7901 tumor cells	1520:1539	SGC-7901 tumor cells	1520:1539	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	9	57	theme	acid	1195:1198	arg1	residue					1200:1206	uronic acid residue	1188:1206	uronic acid residue	1188:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	1	58	from	Haitanensis	241:251	arg1	polysaccharides					211:225	extract polysaccharides	203:225	extract polysaccharides from Porphyra Haitanensis (PHP) with water	203:268	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	0	59	from	haitanensis	131:141	arg1	activities					87:96	antitumor activities	77:96	antitumor activities	77:96	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	0	59	from	haitanensis	131:141	arg1	polysaccharides					101:115	polysaccharides	101:115	polysaccharides from porphyra haitanensis	101:141	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	0	59	from	haitanensis	131:141	arg1	extraction					35:44	microwave assisted extraction	16:44	microwave assisted extraction	16:44	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	0	59	from	haitanensis	131:141	arg1	characterization					56:71	chemical characterization	47:71	chemical characterization	47:71	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	5	60	theme	chemical	658:665	arg1	monosaccharide					681:694	monosaccharide	681:694	monosaccharide	681:694	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	5	60	theme	chemical	658:665	arg1	gravimetric					708:718	gravimetric	708:718	gravimetric	708:718	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	5	60	theme	chemical	658:665	arg1	compositions					667:678	chemical compositions	658:678	chemical compositions	658:678	Chemical characterization of PHP was investigated based on analysis of chemical compositions, monosaccharide and thermal gravimetric.					
30553311	1	61	theme	assisted	154:161	arg1	technology					180:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology	144:189	Microwave assisted extraction (MAE) technology was used to extract polysaccharides from Porphyra Haitanensis (PHP) with water.					
30553311	12	62	theme	clinical	1664:1671	arg1	use					1673:1675	clinical use	1664:1675	clinical use	1664:1675	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	12	63	from	potential	1650:1658	arg1	treatment					1702:1710	treatment	1702:1710	treatment	1702:1710	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	12	63	from	potential	1650:1658	arg1	prevention					1687:1696	cancer prevention	1680:1696	cancer prevention	1680:1696	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	0	64	theme	antitumor	77:85	arg1	activities					87:96	antitumor activities	77:96	antitumor activities	77:96	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	7	65	theme	time	943:946	arg1	material					882:889	raw material 28.98	878:895	raw material 28.98(mL/g)	878:901	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	65	theme	time	943:946	arg1	14.14 min					948:956	extraction time 14.14 min	932:956	extraction time 14.14 min	932:956	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	8	66	theme	suitable	969:976	arg1	MAE					959:961	MAE	959:961	MAE	959:961	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	8	66	theme	suitable	969:976	arg1	technique					992:1000	a suitable and efficient technique	967:1000	a suitable and efficient technique for PHP extraction	967:1019	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	11	67	from	apoptosis	1507:1515	arg1	mice					1597:1600	SGC-7901 tumor-bearing mice	1574:1600	SGC-7901 tumor-bearing mice	1574:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	8	68	theme	efficient	982:990	arg1	MAE					959:961	MAE	959:961	MAE	959:961	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	8	68	theme	efficient	982:990	arg1	technique					992:1000	a suitable and efficient technique	967:1000	a suitable and efficient technique for PHP extraction	967:1019	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	7	69	theme	power	914:918	arg1	77.84 W					920:926	microwave power 77.84 W	904:926	microwave power 77.84 W	904:926	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	7	69	theme	power	914:918	arg1	material					882:889	raw material 28.98	878:895	raw material 28.98(mL/g)	878:901	The results indicated that the optimal conditions for MAE of PHP were ratio of water to raw material 28.98(mL/g), microwave power 77.84 W and extraction time 14.14 min.					
30553311	6	70	theme	antitumor	725:733	arg1	activities					735:744	The antitumor activities	721:744	The antitumor activities of PHP in vivo and in vitro	721:772	The antitumor activities of PHP in vivo and in vitro were evaluated.					
30553311	0	71	from	characterization	56:71	arg1	haitanensis					131:141	porphyra haitanensis	122:141	porphyra haitanensis	122:141	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	72	from	glucose	1291:1297	arg1	ratio					1325:1329	a molar ratio	1317:1329	a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1317:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	73	from	mannose	1282:1288	arg1	ratio					1325:1329	a molar ratio	1317:1329	a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1317:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	4	74	theme	MAE	518:520	arg1	process					522:528	MAE process	518:528	MAE process of PHP	518:535	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	0	75	theme	assisted	26:33	arg1	extraction					35:44	microwave assisted extraction	16:44	microwave assisted extraction	16:44	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	8	76	theme	hot	1047:1049	arg1	extraction					1057:1066	traditional hot water extraction	1035:1066	traditional hot water extraction	1035:1066	MAE was a suitable and efficient technique for PHP extraction compared with traditional hot water extraction.					
30553311	4	77	theme	single	492:497	arg1	experiment					506:515	single factor experiment	492:515	single factor experiment	492:515	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	12	78	contain	had	1642:1644	arg2	potential					1650:1658	the potential	1646:1658	the potential for clinical use in cancer prevention and treatment	1646:1710	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	12	78	contain	had	1642:1644	arg1	PHP					1638:1640	PHP	1638:1640	PHP	1638:1640	The results suggested that PHP had the potential for clinical use in cancer prevention and treatment.					
30553311	11	79	theme	antitumor	1554:1562	arg1	effect					1564:1569	antitumor effect	1554:1569	antitumor effect on SGC-7901 tumor-bearing mice	1554:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	0	80	theme	characterization	56:71	arg1	Optimization					0:11	Optimization	0:11	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.	0:142	Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.					
30553311	9	81	contain	contained	1123:1131	arg2	proteins					1215:1222	no proteins	1212:1222	no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1212:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	81	contain	contained	1123:1131	arg2	%					1183:1183	24.63 ± 1.69%	1171:1183	24.63 ± 1.69% of uronic acid residue	1171:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	81	contain	contained	1123:1131	arg1	PHP					1119:1121	PHP	1119:1121	PHP	1119:1121	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	81	contain	contained	1123:1131	arg2	%					1145:1145	75.36 ± 1.48%	1133:1145	75.36 ± 1.48% of total carbohydrates	1133:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	81	contain	contained	1123:1131	arg2	carbohydrates					1156:1168	total carbohydrates	1150:1168	total carbohydrates	1150:1168	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	81	contain	contained	1123:1131	arg2	residue					1200:1206	uronic acid residue	1188:1206	uronic acid residue	1188:1206	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	9	82	theme	characterization	1090:1105	arg1	Analysis					1069:1076	Analysis	1069:1076	Analysis of chemical characterization	1069:1105	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
30553311	4	83	theme	surface	566:572	arg1	methodology					574:584	response surface methodology	557:584	response surface methodology	557:584	Based on single factor experiment, MAE process of PHP was optimized using response surface methodology.					
30553311	11	84	theme	tumor-bearing	1583:1595	arg1	mice					1597:1600	SGC-7901 tumor-bearing mice	1574:1600	SGC-7901 tumor-bearing mice	1574:1600	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	11	85	contain	had	1450:1452	arg1	PHP					1446:1448	PHP	1446:1448	PHP	1446:1448	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	11	85	contain	had	1450:1452	arg2	effect					1462:1467	obvious effect	1454:1467	obvious effect	1454:1467	PHP had obvious effect on inhibiting proliferation, inducing apoptosis of SGC-7901 tumor cells in vitro and antitumor effect on SGC-7901 tumor-bearing mice in vivo.					
30553311	9	86	theme	10.25:9.38:1:12.45:9.9:11.55	1334:1361	arg1	ratio					1325:1329	a molar ratio	1317:1329	a molar ratio of 10.25:9.38:1:12.45:9.9:11.55	1317:1361	Analysis of chemical characterization showed that PHP contained 75.36 ± 1.48% of total carbohydrates, 24.63 ± 1.69% of uronic acid residue and no proteins with monosaccharides such as rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 10.25:9.38:1:12.45:9.9:11.55.					
29306327	6	0	theme	mannose	1172:1178	arg1	percentage					1180:1189	highest mannose percentage	1164:1189	highest mannose percentage of 62.76 %	1164:1200	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	3	1	theme	morphological	758:770	arg1	changes					772:778	morphological changes	758:778	morphological changes	758:778	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	2	2	theme	titanium	314:321	arg1	oxide					323:327	titanium oxide	314:327	titanium oxide	314:327	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	7	3	dep	production	1637:1646	arg1	the					1618:1620	the	1618:1620	the	1618:1620	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	7	4	from	compositions/percentages	1658:1681	arg1	exo-polysaccharides					1699:1717	exo-polysaccharides	1699:1717	exo-polysaccharides	1699:1717	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	7	4	from	compositions/percentages	1658:1681	arg1	mycelial					1686:1693	mycelial	1686:1693	mycelial	1686:1693	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	5	from	compositions/percentages	1095:1118	arg1	polysaccharide					1144:1157	G. frondosa mycelial polysaccharide	1123:1157	G. frondosa mycelial polysaccharide	1123:1157	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	5	6	theme	g/L	944:946	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	6	theme	g/L	944:946	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	6	theme	g/L	944:946	arg1	exo-polysaccharide					952:969	exo-polysaccharide	952:969	exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc	952:1000	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	7	theme	UDP-glucose	1347:1357	arg1	UGP					1378:1380	UGP	1378:1380	UGP	1378:1380	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	7	theme	UDP-glucose	1347:1357	arg1	pyrophosphorylase					1359:1375	UDP-glucose pyrophosphorylase	1347:1375	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	2	8	theme	mycelial	343:350	arg1	morphologies					352:363	mycelial morphologies	343:363	mycelial morphologies	343:363	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	3	9	theme	polysaccharide	621:634	arg1	synthesis					636:644	polysaccharide synthesis	621:644	polysaccharide synthesis in G. frondosa	621:659	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	5	10	theme	mycelial	918:925	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	7	11	theme	G.	1814:1815	arg1	frondosa					1817:1824	G. frondosa	1814:1824	G. frondosa	1814:1824	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	12	theme	lowest	1340:1345	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	13	theme	dehydrogenase	1431:1443	arg1	activities					1494:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities	1411:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1411:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	5	14	from	biomass	927:933	arg1	g/L					990:992	6.0 g/L	986:992	6.0 g/L	986:992	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	15	theme	enzymes	1322:1328	arg1	changes					1275:1281	the corresponding changes	1257:1281	the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1257:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	16	theme	highest	1411:1417	arg1	UGDG					1446:1449	UGDG	1446:1449	UGDG	1446:1449	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	16	theme	highest	1411:1417	arg1	GMPPB					1487:1491	GMPPB	1487:1491	GMPPB	1487:1491	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	16	theme	highest	1411:1417	arg1	dehydrogenase					1431:1443	highest UDP-glucose dehydrogenase	1411:1443	highest UDP-glucose dehydrogenase (UGDG)	1411:1450	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	7	17	theme	related	1784:1790	arg1	enzymes					1792:1798	polysaccharide-biosynthesis related enzymes	1756:1798	polysaccharide-biosynthesis related enzymes	1756:1798	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	18	theme	polysaccharide-synthesis	1286:1309	arg1	enzymes					1322:1328	polysaccharide-synthesis associated enzymes	1286:1328	polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1286:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	18	theme	polysaccharide-synthesis	1286:1309	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	19	theme	mU/mg	1401:1405	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	5	20	theme	Talc	997:1000	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	20	theme	Talc	997:1000	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	20	theme	Talc	997:1000	arg1	exo-polysaccharide					952:969	exo-polysaccharide	952:969	exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc	952:1000	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	21	theme	activities	1494:1503	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	7	22	theme	mycelial	1732:1739	arg1	morphology					1741:1750	mycelial morphology	1732:1750	mycelial morphology	1732:1750	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	1	23	theme	mycelial	178:185	arg1	morphologies					187:198	filamentous mycelial morphologies	166:198	filamentous mycelial morphologies	166:198	BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation.					
29306327	0	24	theme	microparticle	98:110	arg1	Talc					112:115	microparticle Talc	98:115	microparticle Talc	98:115	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	6	25	dep	altered	1068:1074	arg1	followed					1243:1250	followed	1243:1250	followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1243:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	26	theme	frondosa	1126:1133	arg1	polysaccharide					1144:1157	G. frondosa mycelial polysaccharide	1123:1157	G. frondosa mycelial polysaccharide	1123:1157	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	2	27	theme	metabolites	405:415	arg1	production					417:426	mycelia growth and secondary metabolites production	376:426	mycelia growth and secondary metabolites production	376:426	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	3	28	theme	mycelial	488:495	arg1	morphology					497:506	mycelial morphology	488:506	mycelial morphology	488:506	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	6	29	theme	mU/mg	1530:1534	arg1	mU/mg					1401:1405	91.18 mU/mg	1395:1405	91.18 mU/mg	1395:1405	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	29	theme	mU/mg	1530:1534	arg1	activities					1494:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities	1411:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1411:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	1	30	theme	BACKGROUND	118:127	arg1	Mushroom					129:136	BACKGROUND Mushroom	118:136	BACKGROUND Mushroom	118:136	BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation.					
29306327	3	31	theme	Talc	459:462	arg1	addition					472:479	microparticle Talc (45 μm) addition	445:479	microparticle Talc (45 μm) addition	445:479	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	31	theme	Talc	459:462	arg1	μm					468:469	45 μm	465:469	45 μm	465:469	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	32	theme	relationship	705:716	arg1	clue					693:696	a clue	691:696	a clue of the relationship between polysaccharide biosynthesis and morphological changes	691:778	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	6	33	theme	monosaccharide	1080:1093	arg1	compositions/percentages					1095:1118	the monosaccharide compositions/percentages	1076:1118	the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide	1076:1157	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	2	34	theme	growth	384:389	arg1	production					417:426	mycelia growth and secondary metabolites production	376:426	mycelia growth and secondary metabolites production	376:426	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	0	35	theme	Grifola	50:56	arg1	frondosa					58:65	Grifola frondosa	50:65	Grifola frondosa	50:65	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	7	36	theme	polysaccharide	1622:1635	arg1	production					1637:1646	polysaccharide production	1622:1646	polysaccharide production	1622:1646	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	5	37	from	polysaccharide	1016:1029	arg1	g/L					990:992	6.0 g/L	986:992	6.0 g/L	986:992	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	3	38	theme	polysaccharide	726:739	arg1	biosynthesis					741:752	polysaccharide biosynthesis	726:752	polysaccharide biosynthesis	726:752	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	4	39	theme	mycelia	887:893	arg1	percentage					862:871	the percentage	858:871	the percentage of S-fraction mycelia	858:893	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	6	40	theme	mU/mg	1514:1518	arg1	mU/mg					1401:1405	91.18 mU/mg	1395:1405	91.18 mU/mg	1395:1405	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	40	theme	mU/mg	1514:1518	arg1	activities					1494:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities	1411:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1411:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	7	41	theme	Talc	1591:1594	arg1	presence					1579:1586	the presence	1575:1586	the presence of Talc	1575:1594	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	5	42	theme	Talc	1057:1060	arg1	g/L					1050:1052	3.0 g/L	1046:1052	3.0 g/L of Talc	1046:1060	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	43	theme	91.18	1395:1399	arg1	mU/mg					1401:1405	91.18 mU/mg	1395:1405	91.18 mU/mg	1395:1405	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	2	44	theme	secondary	395:403	arg1	metabolites					405:415	secondary metabolites	395:415	secondary metabolites	395:415	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	7	45	from	production	1637:1646	arg1	exo-polysaccharides					1699:1717	exo-polysaccharides	1699:1717	exo-polysaccharides	1699:1717	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	7	45	from	production	1637:1646	arg1	mycelial					1686:1693	mycelial	1686:1693	mycelial	1686:1693	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	46	theme	highest	1164:1170	arg1	percentage					1180:1189	highest mannose percentage	1164:1189	highest mannose percentage of 62.76 %	1164:1200	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	0	47	with	morphology	82:91	arg1	Talc					112:115	microparticle Talc	98:115	microparticle Talc	98:115	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	5	48	theme	g/L	979:981	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	48	theme	g/L	979:981	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	48	theme	g/L	979:981	arg1	exo-polysaccharide					952:969	exo-polysaccharide	952:969	exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc	952:1000	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	49	theme	81.45	1508:1512	arg1	mU/mg					1514:1518	81.45 mU/mg	1508:1518	81.45 mU/mg	1508:1518	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	50	theme	%	1241:1241	arg1	percentage					1180:1189	highest mannose percentage	1164:1189	highest mannose percentage of 62.76 %	1164:1200	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	50	theme	%	1241:1241	arg1	percentage					1221:1230	lowest glucose percentage	1206:1230	lowest glucose percentage of 15.22 %	1206:1241	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	51	theme	pyrophosphorylase	1359:1375	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	1	52	theme	submerged	207:215	arg1	fermentation					217:228	submerged fermentation	207:228	submerged fermentation	207:228	BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation.					
29306327	7	53	theme	frondosa	1817:1824	arg1	activities					1800:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities	1732:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa	1732:1824	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	5	54	theme	maximum	910:916	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	55	theme	glucose	1213:1219	arg1	percentage					1221:1230	lowest glucose percentage	1206:1230	lowest glucose percentage of 15.22 %	1206:1241	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	3	56	theme	monosaccharide	535:548	arg1	compositions					550:561	monosaccharide compositions	535:561	monosaccharide compositions of polysaccharides	535:580	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	2	57	theme	magnesium	274:282	arg1	Talc					268:271	Talc	268:271	Talc (magnesium silicate)	268:292	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	2	57	theme	magnesium	274:282	arg1	silicate					284:291	magnesium silicate	274:291	magnesium silicate	274:291	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	7	58	theme	enzymes	1792:1798	arg1	activities					1800:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities	1732:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa	1732:1824	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	59	theme	UDP-glucose	1419:1429	arg1	UGDG					1446:1449	UGDG	1446:1449	UGDG	1446:1449	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	59	theme	UDP-glucose	1419:1429	arg1	GMPPB					1487:1491	GMPPB	1487:1491	GMPPB	1487:1491	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	59	theme	UDP-glucose	1419:1429	arg1	dehydrogenase					1431:1443	highest UDP-glucose dehydrogenase	1411:1443	highest UDP-glucose dehydrogenase (UGDG)	1411:1450	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	3	60	theme	G.	649:650	arg1	frondosa					652:659	G. frondosa	649:659	G. frondosa	649:659	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	7	61	theme	polysaccharide-biosynthesis	1756:1782	arg1	enzymes					1792:1798	polysaccharide-biosynthesis related enzymes	1756:1798	polysaccharide-biosynthesis related enzymes	1756:1798	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	62	theme	associated	1311:1320	arg1	enzymes					1322:1328	polysaccharide-synthesis associated enzymes	1286:1328	polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1286:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	62	theme	associated	1311:1320	arg1	activity					1383:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity	1340:1390	lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1340:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	2	63	theme	aluminum	295:302	arg1	oxide					304:308	aluminum oxide	295:308	aluminum oxide	295:308	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	7	64	dep	CONCLUSION	1537:1546	arg1	revealed					1561:1568	revealed	1561:1568	revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa	1561:1824	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	7	65	theme	morphology	1741:1750	arg1	activities					1800:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities	1732:1809	mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa	1732:1824	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	4	66	theme	Talc	801:804	arg1	Addition					789:796	Addition	789:796	Addition of Talc	789:804	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	3	67	from	synthesis	636:644	arg1	frondosa					652:659	G. frondosa	649:659	G. frondosa	649:659	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	5	68	from	exo-polysaccharide	952:969	arg1	g/L					990:992	6.0 g/L	986:992	6.0 g/L	986:992	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	69	theme	corresponding	1261:1273	arg1	changes					1275:1281	the corresponding changes	1257:1281	the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1257:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	70	theme	pyrophosphorylase	1468:1484	arg1	activities					1494:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities	1411:1503	highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg	1411:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	1	71	theme	filamentous	166:176	arg1	morphologies					187:198	filamentous mycelial morphologies	166:198	filamentous mycelial morphologies	166:198	BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation.					
29306327	4	72	dep	RESULTS	781:787	arg1	increased					848:856	increased	848:856	increased the percentage of S-fraction mycelia	848:893	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	4	72	dep	RESULTS	781:787	arg1	decreased					806:814	decreased	806:814	decreased the diameter of the pellets	806:842	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	4	73	theme	pellets	836:842	arg1	diameter					820:827	the diameter	816:827	the diameter of the pellets	816:842	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	6	74	theme	GDP-mannose	1456:1466	arg1	pyrophosphorylase					1468:1484	GDP-mannose pyrophosphorylase	1456:1484	GDP-mannose pyrophosphorylase	1456:1484	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	5	75	theme	g/g	1039:1041	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	75	theme	g/g	1039:1041	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	75	theme	g/g	1039:1041	arg1	exo-polysaccharide					952:969	exo-polysaccharide	952:969	exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc	952:1000	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	3	76	theme	addition	472:479	arg1	effect					435:440	effect	435:440	effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa	435:659	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	77	from	effect	435:440	arg1	performance					522:532	fermentation performance	509:532	fermentation performance	509:532	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	77	from	effect	435:440	arg1	compositions					550:561	monosaccharide compositions	535:561	monosaccharide compositions of polysaccharides	535:580	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	77	from	effect	435:440	arg1	enzymes					586:592	enzymes	586:592	enzymes	586:592	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	77	from	effect	435:440	arg1	morphology					497:506	mycelial morphology	488:506	mycelial morphology	488:506	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	6	78	theme	mycelial	1135:1142	arg1	polysaccharide					1144:1157	G. frondosa mycelial polysaccharide	1123:1157	G. frondosa mycelial polysaccharide	1123:1157	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	0	79	theme	polysaccharide	21:34	arg1	production					36:45	polysaccharide production	21:45	polysaccharide production	21:45	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	5	80	from	g/L	990:992	arg1	biomass					927:933	the maximum mycelial biomass	906:933	the maximum mycelial biomass of 19.25 g/L	906:946	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	80	from	g/L	990:992	arg1	g/L					979:981	3.12 g/L	974:981	3.12 g/L at 6.0 g/L	974:992	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	80	from	g/L	990:992	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	80	from	g/L	990:992	arg1	exo-polysaccharide					952:969	exo-polysaccharide	952:969	exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc	952:1000	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	5	81	theme	mycelial	1007:1014	arg1	polysaccharide					1016:1029	mycelial polysaccharide	1007:1029	mycelial polysaccharide of 0.24 g/g	1007:1041	Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc.					
29306327	6	82	theme	G.	1123:1124	arg1	polysaccharide					1144:1157	G. frondosa mycelial polysaccharide	1123:1157	G. frondosa mycelial polysaccharide	1123:1157	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	83	theme	93.15	1524:1528	arg1	mU/mg					1530:1534	93.15 mU/mg	1524:1534	93.15 mU/mg	1524:1534	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	0	84	theme	frondosa	58:65	arg1	mycelia					9:15	mycelia	9:15	mycelia	9:15	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	0	84	theme	frondosa	58:65	arg1	production					36:45	polysaccharide production	21:45	polysaccharide production	21:45	Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc.					
29306327	7	85	theme	sugar	1652:1656	arg1	compositions/percentages					1658:1681	sugar compositions/percentages	1652:1681	sugar compositions/percentages	1652:1681	CONCLUSION Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.					
29306327	6	86	theme	lowest	1206:1211	arg1	percentage					1221:1230	lowest glucose percentage	1206:1230	lowest glucose percentage of 15.22 %	1206:1241	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	3	87	theme	microparticle	445:457	arg1	addition					472:479	microparticle Talc (45 μm) addition	445:479	microparticle Talc (45 μm) addition	445:479	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	87	theme	microparticle	445:457	arg1	μm					468:469	45 μm	465:469	45 μm	465:469	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	2	88	theme	microparticles	243:256	arg1	Addition					231:238	Addition	231:238	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide	231:327	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	2	89	theme	mycelia	376:382	arg1	growth					384:389	mycelia growth	376:389	mycelia growth	376:389	Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production.					
29306327	3	90	dep	morphology	497:506	arg1	activities					594:603	activities	594:603	activities	594:603	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	90	dep	morphology	497:506	arg1	the					484:486	the	484:486	the	484:486	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	6	91	theme	%	1200:1200	arg1	percentage					1180:1189	highest mannose percentage	1164:1189	highest mannose percentage of 62.76 %	1164:1200	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	6	91	theme	%	1200:1200	arg1	percentage					1221:1230	lowest glucose percentage	1206:1230	lowest glucose percentage of 15.22 %	1206:1241	Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg.					
29306327	3	92	theme	fermentation	509:520	arg1	performance					522:532	fermentation performance	509:532	fermentation performance	509:532	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	4	93	theme	S-fraction	876:885	arg1	mycelia					887:893	S-fraction mycelia	876:893	S-fraction mycelia	876:893	RESULTS Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia.					
29306327	3	94	theme	polysaccharides	566:580	arg1	performance					522:532	fermentation performance	509:532	fermentation performance	509:532	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	94	theme	polysaccharides	566:580	arg1	compositions					550:561	monosaccharide compositions	535:561	monosaccharide compositions of polysaccharides	535:580	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	94	theme	polysaccharides	566:580	arg1	enzymes					586:592	enzymes	586:592	enzymes	586:592	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29306327	3	94	theme	polysaccharides	566:580	arg1	morphology					497:506	mycelial morphology	488:506	mycelial morphology	488:506	Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes.					
29972101	25	0	theme	low	3718:3720	arg1	temperature					3722:3732	low temperature	3718:3732	low temperature	3718:3732	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	8	1	theme	new	1035:1037	arg1	result					1072:1077	a result	1070:1077	a result of transglycosylation	1070:1099	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	8	1	theme	new	1035:1037	arg1	linkage					1049:1055	a new glycoside linkage	1033:1055	a new glycoside linkage	1033:1055	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	11	2	theme	E119G	1474:1478	arg1	mutant					1480:1485	the E119G mutant	1470:1485	the E119G mutant of endo-1,3-β-glucanase	1470:1509	METHOD In this article, we generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1.					
29972101	7	3	with	reaction	867:874	arg1	intermediate					913:924	the substrate - enzyme intermediate	890:924	intermediate	913:924	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	7	4	theme	sugar	968:972	arg1	production					941:950	the production	937:950	the production of a hydrolyzed sugar	937:972	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	14	5	theme	conditions	1864:1873	arg1	effect					1845:1850	the effect	1841:1850	the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose	1841:1957	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	18	6	theme	transglycosylation	2583:2600	arg1	products					2602:2609	transglycosylation products	2583:2609	transglycosylation products	2583:2609	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	6	7	theme	activity	821:828	arg1	loss					802:805	the loss	798:805	the loss of hydrolytic activity	798:828	The substitution of catalytic residue in glycosidase results in the loss of hydrolytic activity.					
29972101	11	8	theme	cellulans	1535:1543	arg1	DK-1					1545:1548	Cellulosimicrobium cellulans DK-1	1516:1548	Cellulosimicrobium cellulans DK-1	1516:1548	METHOD In this article, we generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1.					
29972101	19	9	theme	transglycosylation	2719:2736	arg1	activity					2738:2745	the transglycosylation activity	2715:2745	the transglycosylation activity	2715:2745	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	9	10	dep	OBJECTIVE	1102:1110	arg1	evaluated					1132:1140	evaluated	1132:1140	evaluated	1132:1140	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	2	11	theme	oligosaccharide	299:313	arg1	synthesis					315:323	oligosaccharide synthesis	299:323	oligosaccharide synthesis by conventional methods	299:347	However, owing to difficulties in oligosaccharide synthesis by conventional methods, oligosaccharide assembly using enzymes has been explored.					
29972101	1	12	theme	wide	204:207	arg1	range					209:213	a wide range	202:213	a wide range of therapeutic strategies in medical specialties	202:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	15	13	theme	enzymatic	1984:1992	arg1	activity					1994:2001	the enzymatic activity	1980:2001	the enzymatic activity of E119G toward laminaritriose	1980:2032	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	15	14	theme	glucose	2053:2059	arg1	presence					2041:2048	the presence	2037:2048	the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity	2037:2175	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	2	15	theme	oligosaccharide	350:364	arg1	assembly					366:373	oligosaccharide assembly	350:373	oligosaccharide assembly using enzymes	350:387	However, owing to difficulties in oligosaccharide synthesis by conventional methods, oligosaccharide assembly using enzymes has been explored.					
29972101	20	16	theme	pH	2806:2807	arg1	range					2797:2801	the range	2793:2801	the range of pH 4.0 - 7.4	2793:2817	The enzymatic activities were similar within the range of pH 4.0 - 7.4, while those at pH 8.0 and 8.5 were slightly decreased.					
29972101	24	17	theme	structural	3418:3427	arg1	information					3429:3439	the structural information	3414:3439	the structural information previously reported	3414:3459	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	10	18	from	effect	1361:1366	arg1	activity					1423:1430	the transglycosylation activity	1400:1430	the transglycosylation activity	1400:1430	We also analyzed the effect of glucose and laminaribiose on the transglycosylation activity.					
29972101	9	19	theme	wild-type	1322:1330	arg1	enzyme					1332:1337	the wild-type enzyme	1318:1337	the wild-type enzyme	1318:1337	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	1	20	theme	strategies	230:239	arg1	range					209:213	a wide range	202:213	a wide range of therapeutic strategies in medical specialties	202:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	0	21	from	cellulans	103:111	arg1	Mutant					48:53	Catalytic Domain Mutant	31:53	Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans	31:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	0	21	from	cellulans	103:111	arg1	Endo-1,3-β-glucanase					58:77	Endo-1,3-β-glucanase	58:77	Endo-1,3-β-glucanase from Cellulosimicrobium cellulans	58:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	21	22	theme	transglycosylation	3033:3050	arg1	laminaripentaose					3090:3105	laminaripentaose	3090:3105	laminaripentaose	3090:3105	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	21	22	theme	transglycosylation	3033:3050	arg1	products					3052:3059	the transglycosylation products	3029:3059	the transglycosylation products such as laminaritetraose and laminaripentaose	3029:3105	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	21	22	theme	transglycosylation	3033:3050	arg1	laminaritetraose					3069:3084	laminaritetraose	3069:3084	laminaritetraose	3069:3084	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	1	23	theme	medical	244:250	arg1	specialties					252:262	medical specialties	244:262	medical specialties	244:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	7	24	theme	enzyme	906:911	arg1	intermediate					913:924	the substrate - enzyme intermediate	890:924	intermediate	913:924	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	6	25	theme	catalytic	754:762	arg1	residue					764:770	catalytic residue	754:770	catalytic residue	754:770	The substitution of catalytic residue in glycosidase results in the loss of hydrolytic activity.					
29972101	5	26	theme	mutated	634:640	arg1	glycosidase					642:652	mutated glycosidase	634:652	mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity	634:731	There is an additional approach to use mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity.					
29972101	13	27	theme	wild-type	1709:1717	arg1	activities					1695:1704	The enzymatic activities	1681:1704	The enzymatic activities of wild-type and E119G	1681:1727	The enzymatic activities of wild-type and E119G were estimated by detecting the products obtained from laminarioligosaccharides as substrates.					
29972101	1	28	from	range	209:213	arg1	specialties					252:262	medical specialties	244:262	medical specialties	244:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	10	29	theme	transglycosylation	1404:1421	arg1	activity					1423:1430	the transglycosylation activity	1400:1430	the transglycosylation activity	1400:1430	We also analyzed the effect of glucose and laminaribiose on the transglycosylation activity.					
29972101	17	30	theme	relative	2425:2432	arg1	activity					2381:2388	enhanced transglycosylation activity	2353:2388	enhanced transglycosylation activity	2353:2388	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	15	31	theme	additional	2107:2116	arg1	molecules					2118:2126	these additional molecules	2101:2126	these additional molecules	2101:2126	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	23	32	theme	transglycosylation	3372:3389	arg1	activity					3391:3398	the transglycosylation activity	3368:3398	the transglycosylation activity	3368:3398	The combination of laminaribiose and laminaritriose as a substrate enhanced the transglycosylation activity.					
29972101	22	33	dep	CONCLUSION	3108:3117	arg1	exhibited					3225:3233	exhibited	3225:3233	exhibited transglycosylation activity on laminarioligosaccharides	3225:3289	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	4	34	theme	transglycosylases	576:592	arg1	specificity					548:558	specificity	548:558	specificity	548:558	Further studies are required to improve the specificity and activity of transglycosylases.					
29972101	4	34	theme	transglycosylases	576:592	arg1	activity					564:571	activity	564:571	activity	564:571	Further studies are required to improve the specificity and activity of transglycosylases.					
29972101	21	35	theme	E119G	2901:2905	arg1	activity					2889:2896	The enzymatic activity	2875:2896	The enzymatic activity of E119G toward laminaritriose in the presence of glucose	2875:2954	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	21	35	theme	E119G	2901:2905	arg1	ineffective					2960:2970	ineffective	2960:2970	ineffective	2960:2970	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	25	36	theme	product	3667:3673	arg1	amount					3638:3643	the amount	3634:3643	the amount of transglycosylation product	3634:3673	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	25	36	theme	product	3667:3673	arg1	product					3667:3673	transglycosylation product	3648:3673	transglycosylation product	3648:3673	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	1	37	theme	drug	164:167	arg1	programs					179:186	drug discovery programs	164:186	drug discovery programs which address a wide range of therapeutic strategies in medical specialties	164:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	22	38	theme	catalytic	3133:3141	arg1	residue					3143:3149	catalytic residue	3133:3149	catalytic residue	3133:3149	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	22	38	theme	catalytic	3133:3141	arg1	Glu119					3152:3157	Glu119	3152:3157	Glu119 to Gly	3152:3164	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	17	39	theme	transglycosylation	2362:2379	arg1	activity					2381:2388	enhanced transglycosylation activity	2353:2388	enhanced transglycosylation activity	2353:2388	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	9	40	theme	transglycosylation	1146:1163	arg1	activity					1165:1172	the transglycosylation activity	1142:1172	the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides	1142:1243	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	16	41	theme	similar	2296:2302	arg1	structure					2285:2293	the secondary structure	2271:2293	the secondary structure	2271:2293	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	23	42	theme	laminaritriose	3329:3342	arg1	combination					3296:3306	The combination	3292:3306	The combination of laminaribiose and laminaritriose as a substrate	3292:3357	The combination of laminaribiose and laminaritriose as a substrate enhanced the transglycosylation activity.					
29972101	3	43	theme	oligosaccharide	477:491	arg1	synthesis					493:501	the oligosaccharide synthesis	473:501	the oligosaccharide synthesis	473:501	The transglycosylases have been demonstrated to be effective for the oligosaccharide synthesis.					
29972101	0	44	theme	Transglycosylation	0:17	arg1	Activity					19:26	Transglycosylation Activity	0:26	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.	0:112	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	16	45	theme	E119G	2199:2203	arg1	mutant					2205:2210	The purified E119G mutant	2186:2210	RESULTS The purified E119G mutant of endo-1,3-β-glucanase	2178:2234	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	23	46	theme	laminaribiose	3311:3323	arg1	combination					3296:3306	The combination	3292:3306	The combination of laminaribiose and laminaritriose as a substrate	3292:3357	The combination of laminaribiose and laminaritriose as a substrate enhanced the transglycosylation activity.					
29972101	17	47	theme	hydrolytic	2404:2413	arg1	activity					2415:2422	decreased hydrolytic activity	2394:2422	decreased hydrolytic activity	2394:2422	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	9	48	theme	mutant	1198:1203	arg1	activity					1165:1172	the transglycosylation activity	1142:1172	the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides	1142:1243	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	14	49	theme	E119G	1931:1935	arg1	activity					1919:1926	the enzymatic activity	1905:1926	the enzymatic activity of E119G toward laminaritriose	1905:1957	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	18	50	theme	E119G	2511:2515	arg1	activities					2497:2506	The hydrolytic as well as transglycosylation activities	2452:2506	The hydrolytic as well as transglycosylation activities of E119G	2452:2515	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	20	51	theme	7.4	2815:2817	arg1	range					2797:2801	the range	2793:2801	the range of pH 4.0 - 7.4	2793:2817	The enzymatic activities were similar within the range of pH 4.0 - 7.4, while those at pH 8.0 and 8.5 were slightly decreased.					
29972101	0	52	theme	Domain	41:46	arg1	Mutant					48:53	Catalytic Domain Mutant	31:53	Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans	31:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	7	53	theme	water	879:883	arg1	reaction					867:874	reaction	867:874	reaction of water with the substrate - enzyme intermediate	867:924	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	22	54	from	cellulans	3215:3223	arg1	mutation					3121:3128	A mutation	3119:3128	A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans	3119:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	22	54	from	cellulans	3215:3223	arg1	endo-1,3-β-glucanase					3170:3189	endo-1,3-β-glucanase	3170:3189	endo-1,3-β-glucanase from Cellulosimicrobium cellulans	3170:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	21	55	theme	glucose	2948:2954	arg1	presence					2936:2943	the presence	2932:2943	the presence of glucose	2932:2954	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	19	56	theme	reduction	2658:2666	arg1	higher					2695:2700	higher	2695:2700	higher	2695:2700	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	19	56	theme	reduction	2658:2666	arg1	degree					2648:2653	The temperature-dependent degree	2622:2653	The temperature-dependent degree of reduction in hydrolytic activity	2622:2689	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	17	57	theme	E119G	2330:2334	arg1	mutant					2336:2341	The E119G mutant	2326:2341	The E119G mutant	2326:2341	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	9	58	theme	pH	1259:1260	arg1	conditions					1278:1287	various pH and temperature conditions	1251:1287	various pH and temperature conditions	1251:1287	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	8	59	theme	glycoside	1039:1047	arg1	result					1072:1077	a result	1070:1077	a result of transglycosylation	1070:1099	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	8	59	theme	glycoside	1039:1047	arg1	linkage					1049:1055	a new glycoside linkage	1033:1055	a new glycoside linkage	1033:1055	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	9	60	with	comparison	1293:1302	arg1	those					1309:1313	those	1309:1313	those	1309:1313	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	9	61	theme	temperature	1266:1276	arg1	conditions					1278:1287	various pH and temperature conditions	1251:1287	various pH and temperature conditions	1251:1287	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	24	62	dep	subsites	3602:3609	arg1	+2					3618:3619	+2	3618:3619	+2	3618:3619	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	24	62	dep	subsites	3602:3609	arg1	+1					3611:3612	+1	3611:3612	+1	3611:3612	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	11	63	dep	METHOD	1433:1438	arg1	generated					1460:1468	generated	1460:1468	generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1	1460:1548	METHOD In this article, we generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1.					
29972101	11	64	theme	Cellulosimicrobium	1516:1533	arg1	DK-1					1545:1548	Cellulosimicrobium cellulans DK-1	1516:1548	Cellulosimicrobium cellulans DK-1	1516:1548	METHOD In this article, we generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1.					
29972101	2	65	theme	conventional	328:339	arg1	methods					341:347	conventional methods	328:347	conventional methods	328:347	However, owing to difficulties in oligosaccharide synthesis by conventional methods, oligosaccharide assembly using enzymes has been explored.					
29972101	14	66	theme	reaction	1855:1862	arg1	conditions					1864:1873	reaction conditions	1855:1873	reaction conditions such as temperature and pH	1855:1900	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	14	66	theme	reaction	1855:1862	arg1	temperature					1883:1893	temperature	1883:1893	temperature	1883:1893	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	14	66	theme	reaction	1855:1862	arg1	pH					1899:1900	pH	1899:1900	pH	1899:1900	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	16	67	dep	RESULTS	2178:2184	arg1	mutant					2205:2210	The purified E119G mutant	2186:2210	RESULTS The purified E119G mutant of endo-1,3-β-glucanase	2178:2234	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	21	68	theme	laminaribiose	2995:3007	arg1	addition					2983:2990	the addition	2979:2990	the addition of laminaribiose	2979:3007	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	18	69	from	decrease	2536:2543	arg1	temperature					2548:2558	temperature	2548:2558	temperature	2548:2558	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	11	70	theme	endo-1,3-β-glucanase	1490:1509	arg1	mutant					1480:1485	the E119G mutant	1470:1485	the E119G mutant of endo-1,3-β-glucanase	1470:1509	METHOD In this article, we generated the E119G mutant of endo-1,3-β-glucanase from Cellulosimicrobium cellulans DK-1.					
29972101	5	71	theme	hydrolytic	713:722	arg1	activity					724:731	a decreased hydrolytic activity	701:731	a decreased hydrolytic activity	701:731	There is an additional approach to use mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity.					
29972101	13	72	theme	E119G	1723:1727	arg1	activities					1695:1704	The enzymatic activities	1681:1704	The enzymatic activities of wild-type and E119G	1681:1727	The enzymatic activities of wild-type and E119G were estimated by detecting the products obtained from laminarioligosaccharides as substrates.					
29972101	7	73	theme	hydrolyzed	957:966	arg1	sugar					968:972	a hydrolyzed sugar	955:972	a hydrolyzed sugar	955:972	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	15	74	theme	transglycosylation	2149:2166	arg1	activity					2168:2175	the transglycosylation activity	2145:2175	the transglycosylation activity	2145:2175	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	8	75	theme	transglycosylation	1082:1099	arg1	result					1072:1077	a result	1070:1077	a result of transglycosylation	1070:1099	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	8	75	theme	transglycosylation	1082:1099	arg1	linkage					1049:1055	a new glycoside linkage	1033:1055	a new glycoside linkage	1033:1055	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	1	76	theme	therapeutic	218:228	arg1	strategies					230:239	therapeutic strategies	218:239	therapeutic strategies in medical specialties	218:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	13	77	theme	enzymatic	1685:1693	arg1	activities					1695:1704	The enzymatic activities	1681:1704	The enzymatic activities of wild-type and E119G	1681:1727	The enzymatic activities of wild-type and E119G were estimated by detecting the products obtained from laminarioligosaccharides as substrates.					
29972101	18	78	theme	products	2602:2609	arg1	ratio					2574:2578	the ratio	2570:2578	the ratio of transglycosylation products	2570:2609	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	22	79	theme	transglycosylation	3235:3252	arg1	activity					3254:3261	transglycosylation activity	3235:3261	transglycosylation activity	3235:3261	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	14	80	from	effect	1845:1850	arg1	activity					1919:1926	the enzymatic activity	1905:1926	the enzymatic activity of E119G toward laminaritriose	1905:1957	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	6	81	from	substitution	738:749	arg1	glycosidase					775:785	glycosidase	775:785	glycosidase	775:785	The substitution of catalytic residue in glycosidase results in the loss of hydrolytic activity.					
29972101	22	82	from	mutation	3121:3128	arg1	endo-1,3-β-glucanase					3170:3189	endo-1,3-β-glucanase	3170:3189	endo-1,3-β-glucanase from Cellulosimicrobium cellulans	3170:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	22	82	from	mutation	3121:3128	arg1	cellulans					3215:3223	Cellulosimicrobium cellulans	3196:3223	Cellulosimicrobium cellulans	3196:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	15	83	theme	E119G	2006:2010	arg1	activity					1994:2001	the enzymatic activity	1980:2001	the enzymatic activity of E119G toward laminaritriose	1980:2032	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	1	84	theme	great	149:153	arg1	value					155:159	great value	149:159	great value	149:159	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	6	85	theme	residue	764:770	arg1	substitution					738:749	The substitution	734:749	The substitution of catalytic residue in glycosidase	734:785	The substitution of catalytic residue in glycosidase results in the loss of hydrolytic activity.					
29972101	1	86	from	specialties	252:262	arg1	range					209:213	a wide range	202:213	a wide range of therapeutic strategies in medical specialties	202:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	25	87	from	temperature	3722:3732	arg1	effective					3705:3713	effective	3705:3713	effective	3705:3713	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	25	87	from	temperature	3722:3732	arg1	reaction					3680:3687	the reaction	3676:3687	the reaction	3676:3687	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	1	88	from	strategies	230:239	arg1	specialties					252:262	medical specialties	244:262	medical specialties	244:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	4	89	theme	Further	504:510	arg1	studies					512:518	Further studies	504:518	Further studies	504:518	Further studies are required to improve the specificity and activity of transglycosylases.					
29972101	0	90	theme	Cellulosimicrobium	84:101	arg1	cellulans					103:111	Cellulosimicrobium cellulans	84:111	Cellulosimicrobium cellulans	84:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	1	91	theme	discovery	169:177	arg1	programs					179:186	drug discovery programs	164:186	drug discovery programs which address a wide range of therapeutic strategies in medical specialties	164:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	5	92	theme	additional	607:616	arg1	approach					618:625	an additional approach	604:625	an additional approach to use mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity	604:731	There is an additional approach to use mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity.					
29972101	19	93	theme	temperature-dependent	2626:2646	arg1	higher					2695:2700	higher	2695:2700	higher	2695:2700	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	19	93	theme	temperature-dependent	2626:2646	arg1	degree					2648:2653	The temperature-dependent degree	2622:2653	The temperature-dependent degree of reduction in hydrolytic activity	2622:2689	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	25	94	theme	transglycosylation	3648:3665	arg1	product					3667:3673	transglycosylation product	3648:3673	transglycosylation product	3648:3673	To increase the amount of transglycosylation product, the reaction was found to be effective at low temperature.					
29972101	9	95	theme	various	1251:1257	arg1	conditions					1278:1287	various pH and temperature conditions	1251:1287	various pH and temperature conditions	1251:1287	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	24	96	from	-3	3531:3532	arg1	subsites					3511:3518	the subsites	3507:3518	the subsites from -1 to -3	3507:3532	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	22	97	theme	residue	3143:3149	arg1	mutation					3121:3128	A mutation	3119:3128	A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans	3119:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	17	98	theme	enhanced	2353:2360	arg1	activity					2381:2388	enhanced transglycosylation activity	2353:2388	enhanced transglycosylation activity	2353:2388	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	6	99	theme	hydrolytic	810:819	arg1	activity					821:828	hydrolytic activity	810:828	hydrolytic activity	810:828	The substitution of catalytic residue in glycosidase results in the loss of hydrolytic activity.					
29972101	10	100	theme	laminaribiose	1383:1395	arg1	effect					1361:1366	the effect	1357:1366	the effect of glucose and laminaribiose on the transglycosylation activity	1357:1430	We also analyzed the effect of glucose and laminaribiose on the transglycosylation activity.					
29972101	5	101	theme	decreased	703:711	arg1	activity					724:731	a decreased hydrolytic activity	701:731	a decreased hydrolytic activity	701:731	There is an additional approach to use mutated glycosidase which transforms into glycosyltransferase with a decreased hydrolytic activity.					
29972101	18	102	theme	transglycosylation	2478:2495	arg1	activities					2497:2506	The hydrolytic as well as transglycosylation activities	2452:2506	The hydrolytic as well as transglycosylation activities of E119G	2452:2515	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	0	103	from	Mutant	48:53	arg1	cellulans					103:111	Cellulosimicrobium cellulans	84:111	Cellulosimicrobium cellulans	84:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	16	104	theme	secondary	2275:2283	arg1	structure					2285:2293	the secondary structure	2271:2293	the secondary structure	2271:2293	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	10	105	theme	glucose	1371:1377	arg1	effect					1361:1366	the effect	1357:1366	the effect of glucose and laminaribiose on the transglycosylation activity	1357:1430	We also analyzed the effect of glucose and laminaribiose on the transglycosylation activity.					
29972101	1	106	theme	BACKGROUND	114:123	arg1	Oligosaccharides					125:140	BACKGROUND Oligosaccharides	114:140	BACKGROUND Oligosaccharides	114:140	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	15	107	theme	laminaribiose	2064:2076	arg1	presence					2041:2048	the presence	2037:2048	the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity	2037:2175	We further analyzed the enzymatic activity of E119G toward laminaritriose in the presence of glucose or laminaribiose to investigate whether these additional molecules could accelerate the transglycosylation activity.					
29972101	21	108	theme	enzymatic	2879:2887	arg1	activity					2889:2896	The enzymatic activity	2875:2896	The enzymatic activity of E119G toward laminaritriose in the presence of glucose	2875:2954	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	21	108	theme	enzymatic	2879:2887	arg1	ineffective					2960:2970	ineffective	2960:2970	ineffective	2960:2970	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	17	109	theme	decreased	2394:2402	arg1	activity					2415:2422	decreased hydrolytic activity	2394:2422	decreased hydrolytic activity	2394:2422	The E119G mutant exhibited enhanced transglycosylation activity and decreased hydrolytic activity, relative to the wild-type.					
29972101	9	110	theme	endo-1,3-β-glucanase	1177:1196	arg1	mutant					1198:1203	endo-1,3-β-glucanase mutant	1177:1203	endo-1,3-β-glucanase mutant	1177:1203	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	9	110	theme	endo-1,3-β-glucanase	1177:1196	arg1	E119G					1206:1210	E119G	1206:1210	E119G	1206:1210	OBJECTIVE In this article, we evaluated the transglycosylation activity of endo-1,3-β-glucanase mutant, E119G, toward laminarioligosaccharides under various pH and temperature conditions, in comparison with those of the wild-type enzyme.					
29972101	0	111	theme	Catalytic	31:39	arg1	Mutant					48:53	Catalytic Domain Mutant	31:53	Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans	31:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	4	112	dep	specificity	548:558	arg1	the					544:546	the	544:546	the	544:546	Further studies are required to improve the specificity and activity of transglycosylases.					
29972101	1	113	from	value	155:159	arg1	programs					179:186	drug discovery programs	164:186	drug discovery programs which address a wide range of therapeutic strategies in medical specialties	164:262	BACKGROUND Oligosaccharides are of great value in drug discovery programs which address a wide range of therapeutic strategies in medical specialties.					
29972101	8	114	theme	water	984:988	arg1	molecule					990:997	the water molecule	980:997	the water molecule	980:997	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	16	115	theme	purified	2190:2197	arg1	mutant					2205:2210	The purified E119G mutant	2186:2210	RESULTS The purified E119G mutant of endo-1,3-β-glucanase	2178:2234	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	0	116	theme	Mutant	48:53	arg1	Activity					19:26	Transglycosylation Activity	0:26	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.	0:112	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	7	117	theme	substrate	894:902	arg1	intermediate					913:924	the substrate - enzyme intermediate	890:924	intermediate	913:924	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	2	118	from	difficulties	283:294	arg1	synthesis					315:323	oligosaccharide synthesis	299:323	oligosaccharide synthesis by conventional methods	299:347	However, owing to difficulties in oligosaccharide synthesis by conventional methods, oligosaccharide assembly using enzymes has been explored.					
29972101	0	119	theme	Endo-1,3-β-glucanase	58:77	arg1	Mutant					48:53	Catalytic Domain Mutant	31:53	Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans	31:111	Transglycosylation Activity of Catalytic Domain Mutant of Endo-1,3-β-glucanase from Cellulosimicrobium cellulans.					
29972101	14	120	theme	enzymatic	1909:1917	arg1	activity					1919:1926	the enzymatic activity	1905:1926	the enzymatic activity of E119G toward laminaritriose	1905:1957	We also analyzed the effect of reaction conditions such as temperature and pH on the enzymatic activity of E119G toward laminaritriose.					
29972101	16	121	theme	endo-1,3-β-glucanase	2215:2234	arg1	mutant					2205:2210	The purified E119G mutant	2186:2210	RESULTS The purified E119G mutant of endo-1,3-β-glucanase	2178:2234	RESULTS The purified E119G mutant of endo-1,3-β-glucanase was properly folded, and exhibited the secondary structure, similar to that of wild-type.					
29972101	19	122	from	degree	2648:2653	arg1	activity					2682:2689	hydrolytic activity	2671:2689	hydrolytic activity	2671:2689	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	21	123	from	activity	2889:2896	arg1	presence					2936:2943	the presence	2932:2943	the presence of glucose	2932:2954	The enzymatic activity of E119G toward laminaritriose in the presence of glucose was ineffective, while the addition of laminaribiose evidently increased the transglycosylation products such as laminaritetraose and laminaripentaose.					
29972101	19	124	theme	hydrolytic	2671:2680	arg1	activity					2682:2689	hydrolytic activity	2671:2689	hydrolytic activity	2671:2689	The temperature-dependent degree of reduction in hydrolytic activity was higher than that in the transglycosylation activity.					
29972101	8	125	theme	competing	1016:1024	arg1	sugar					1026:1030	a competing sugar	1014:1030	a competing sugar	1014:1030	When the water molecule is replaced by a competing sugar, a new glycoside linkage is formed as a result of transglycosylation.					
29972101	22	126	theme	Cellulosimicrobium	3196:3213	arg1	cellulans					3215:3223	Cellulosimicrobium cellulans	3196:3223	Cellulosimicrobium cellulans	3196:3223	CONCLUSION A mutation of catalytic residue, Glu119 to Gly, in endo-1,3-β-glucanase from Cellulosimicrobium cellulans exhibited transglycosylation activity on laminarioligosaccharides.					
29972101	7	127	with	reaction	842:849	arg1	glucanase					856:864	glucanase	856:864	glucanase	856:864	During the reaction with glucanase, reaction of water with the substrate - enzyme intermediate results in the production of a hydrolyzed sugar.					
29972101	24	128	dep	-3	3531:3532	arg1	to					3528:3529	to	3528:3529	to	3528:3529	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	18	129	theme	hydrolytic	2456:2465	arg1	activities					2497:2506	The hydrolytic as well as transglycosylation activities	2452:2506	The hydrolytic as well as transglycosylation activities of E119G	2452:2515	The hydrolytic as well as transglycosylation activities of E119G decreased with the decrease in temperature, however, the ratio of transglycosylation products increased.					
29972101	24	130	theme	reported	3452:3459	arg1	information					3429:3439	the structural information	3414:3439	the structural information previously reported	3414:3459	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	24	131	with	link	3546:3549	arg1	laminaribiose					3556:3568	laminaribiose	3556:3568	laminaribiose	3556:3568	According to the structural information previously reported, laminaritriose mainly binds to the enzyme at the subsites from -1 to -3 and forms a link with laminaribiose, which transiently binds to the subsites +1 and +2.					
29972101	20	132	theme	enzymatic	2752:2760	arg1	activities					2762:2771	The enzymatic activities	2748:2771	The enzymatic activities	2748:2771	The enzymatic activities were similar within the range of pH 4.0 - 7.4, while those at pH 8.0 and 8.5 were slightly decreased.					
29972101	20	132	theme	enzymatic	2752:2760	arg1	similar					2778:2784	similar	2778:2784	similar	2778:2784	The enzymatic activities were similar within the range of pH 4.0 - 7.4, while those at pH 8.0 and 8.5 were slightly decreased.					
30927653	2	0	theme	hemicellulose	341:353	arg1	removal					355:361	90% hemicellulose removal	337:361	90% hemicellulose removal	337:361	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	1	contain	had	237:239	arg2	selectivity					259:269	an extremely good selectivity	241:269	an extremely good selectivity for component fractionation	241:297	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	1	contain	had	237:239	arg1	pretreatment					224:235	EG-AlCl3 pretreatment	215:235	EG-AlCl3 pretreatment	215:235	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	2	with	150 °C	445:450	arg1	AlCl3					469:473	0.055 mol/L AlCl3	457:473	0.055 mol/L AlCl3	457:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	3	theme	%	339:339	arg1	removal					355:361	90% hemicellulose removal	337:361	90% hemicellulose removal	337:361	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	4	dep	residue	491:497	arg1	w/v					504:506	w/v	504:506	w/v	504:506	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	3	4	dep	residue	491:497	arg1	%					501:501	5%	500:501	5%	500:501	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	6	5	theme	due	972:974	arg1	removal					964:970	29% lignin removal	953:970	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	5	6	theme	adsorption	784:793	arg1	ability					795:801	The enzyme adsorption ability	773:801	The enzyme adsorption ability of pretreated straw	773:821	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	5	6	theme	adsorption	784:793	arg1	that					836:839	that	836:839	that	836:839	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	5	7	theme	original	849:856	arg1	sample					858:863	the original sample	845:863	the original sample	845:863	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	0	8	theme	aluminum	83:90	arg1	chloride					92:99	aluminum chloride	83:99	aluminum chloride	83:99	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	2	9	theme	component	275:283	arg1	fractionation					285:297	component fractionation	275:297	component fractionation	275:297	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	10	attach	presented	509:517	arg2	residue					491:497	The pretreated residue	476:497	The pretreated residue (5%, w/v)	476:507	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	3	10	attach	presented	509:517	arg1	loading					617:623	cellulase loading	607:623	cellulase loading of 10 FPU/g	607:635	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	0	11	theme	enzymatic	105:113	arg1	hydrolysis					115:124	enzymatic hydrolysis	105:124	enzymatic hydrolysis	105:124	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	0	12	from	straw	41:45	arg1	recovery					9:16	Complete recovery	0:16	Complete recovery of cellulose from rice straw	0:45	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	6	13	theme	29	953:954	arg1	%					955:955	%	955:955	%	955:955	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	6	14	theme	lignin	957:962	arg1	removal					964:970	29% lignin removal	953:970	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	7	15	theme	little	1048:1053	arg1	loss					1055:1058	little loss	1048:1058	little loss of cellulose	1048:1071	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	2	16	theme	%	372:372	arg1	cellulose					374:382	100% cellulose	369:382	100% cellulose	369:382	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	17	theme	cellulose	407:415	arg1	content					417:423	or 76% (w/w) cellulose content	394:423	content	417:423	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	18	theme	10 FPU/g	628:635	arg1	loading					617:623	cellulase loading	607:623	cellulase loading of 10 FPU/g	607:635	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	0	19	theme	ethylene	63:70	arg1	glycol					72:77	ethylene glycol	63:77	ethylene glycol	63:77	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	2	20	theme	88	313:314	arg1	%					315:315	%	315:315	%	315:315	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	21	from	content	417:423	arg1	150 °C					445:450	150 °C	445:450	150 °C with 0.055 mol/L AlCl3	445:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	21	from	content	417:423	arg1	residue					434:440	solid residue	428:440	solid residue at 150 °C with 0.055 mol/L AlCl3	428:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	22	theme	100	369:371	arg1	%					372:372	%	372:372	%	372:372	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	0	23	theme	Complete	0:7	arg1	recovery					9:16	Complete recovery	0:16	Complete recovery of cellulose from rice straw	0:45	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	4	24	theme	hydrolysis	642:651	arg1	behavior					653:660	The hydrolysis behavior	638:660	The hydrolysis behavior	638:660	The hydrolysis behavior was correlated with the composition and structure of substrates characterized by SEM, FT-IR, BET, XRD and TGA.					
30927653	0	25	theme	cellulose	21:29	arg1	recovery					9:16	Complete recovery	0:16	Complete recovery of cellulose from rice straw	0:45	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	6	26	theme	EG-AlCl3	866:873	arg1	solution					875:882	EG-AlCl3 solution	866:882	EG-AlCl3 solution	866:882	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	6	27	theme	%	955:955	arg1	removal					964:970	29% lignin removal	953:970	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	2	28	theme	%	399:399	arg1	content					417:423	or 76% (w/w) cellulose content	394:423	content	417:423	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	7	29	with	method	1036:1041	arg1	loss					1055:1058	little loss	1048:1058	little loss of cellulose	1048:1071	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	2	30	from	150 °C	445:450	arg1	content					417:423	or 76% (w/w) cellulose content	394:423	content	417:423	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	30	from	150 °C	445:450	arg1	residue					434:440	solid residue	428:440	solid residue at 150 °C with 0.055 mol/L AlCl3	428:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	1	31	theme	ethylene	158:165	arg1	EG					175:176	EG	175:176	EG	175:176	Rice straw was pretreated with ethylene glycol (EG) and AlCl3 for enzymatic hydrolysis.					
30927653	1	31	theme	ethylene	158:165	arg1	glycol					167:172	ethylene glycol	158:172	ethylene glycol (EG)	158:177	Rice straw was pretreated with ethylene glycol (EG) and AlCl3 for enzymatic hydrolysis.					
30927653	0	32	theme	rice	36:39	arg1	straw					41:45	rice straw	36:45	rice straw	36:45	Complete recovery of cellulose from rice straw pretreated with ethylene glycol and aluminum chloride for enzymatic hydrolysis.					
30927653	6	33	theme	AlCl3	991:995	arg1	loss					983:986	the loss	979:986	the loss of AlCl3	979:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	3	34	theme	enzymatic	528:536	arg1	rate					549:552	a higher enzymatic hydrolysis rate	519:552	a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h	519:602	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	6	35	theme	cellulose	925:933	arg1	recovery					935:942	100% cellulose recovery	920:942	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	2	36	theme	solid	428:432	arg1	residue					434:440	solid residue	428:440	solid residue at 150 °C with 0.055 mol/L AlCl3	428:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	37	theme	EG-AlCl3	215:222	arg1	pretreatment					224:235	EG-AlCl3 pretreatment	215:235	EG-AlCl3 pretreatment	215:235	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	1	38	theme	enzymatic	193:201	arg1	hydrolysis					203:212	enzymatic hydrolysis	193:212	enzymatic hydrolysis	193:212	Rice straw was pretreated with ethylene glycol (EG) and AlCl3 for enzymatic hydrolysis.					
30927653	7	39	theme	EG-AlCl3	998:1005	arg1	method					1036:1041	an efficient method	1023:1041	an efficient method with little loss of cellulose for lignocelluloses	1023:1091	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	7	39	theme	EG-AlCl3	998:1005	arg1	pretreatment					1007:1018	EG-AlCl3 pretreatment	998:1018	EG-AlCl3 pretreatment	998:1018	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	7	40	theme	cellulose	1063:1071	arg1	loss					1055:1058	little loss	1048:1058	little loss of cellulose	1048:1071	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	6	41	theme	%	923:923	arg1	recovery					935:942	100% cellulose recovery	920:942	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	2	42	theme	90	337:338	arg1	%					339:339	%	339:339	%	339:339	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	43	theme	higher	521:526	arg1	rate					549:552	a higher enzymatic hydrolysis rate	519:552	a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h	519:602	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	5	44	theme	straw	817:821	arg1	ability					795:801	The enzyme adsorption ability	773:801	The enzyme adsorption ability of pretreated straw	773:821	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	5	44	theme	straw	817:821	arg1	that					836:839	that	836:839	that	836:839	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	7	45	theme	efficient	1026:1034	arg1	method					1036:1041	an efficient method	1023:1041	an efficient method with little loss of cellulose for lignocelluloses	1023:1091	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	7	45	theme	efficient	1026:1034	arg1	pretreatment					1007:1018	EG-AlCl3 pretreatment	998:1018	EG-AlCl3 pretreatment	998:1018	EG-AlCl3 pretreatment is an efficient method with little loss of cellulose for lignocelluloses.					
30927653	4	46	dep	composition	686:696	arg1	the					682:684	the	682:684	the	682:684	The hydrolysis behavior was correlated with the composition and structure of substrates characterized by SEM, FT-IR, BET, XRD and TGA.					
30927653	2	47	theme	good	254:257	arg1	selectivity					259:269	an extremely good selectivity	241:269	an extremely good selectivity for component fractionation	241:297	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	48	theme	hydrolysis	538:547	arg1	rate					549:552	a higher enzymatic hydrolysis rate	519:552	a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h	519:602	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	1	49	theme	Rice	127:130	arg1	straw					132:136	Rice straw	127:136	Rice straw	127:136	Rice straw was pretreated with ethylene glycol (EG) and AlCl3 for enzymatic hydrolysis.					
30927653	6	50	theme	100	920:922	arg1	%					923:923	%	923:923	%	923:923	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	5	51	theme	pretreated	806:815	arg1	straw					817:821	pretreated straw	806:821	pretreated straw	806:821	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	2	52	theme	%	315:315	arg1	delignification					317:331	88% delignification	313:331	88% delignification	313:331	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	2	53	theme	0.055 mol/L	457:467	arg1	AlCl3					469:473	0.055 mol/L AlCl3	457:473	0.055 mol/L AlCl3	457:473	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	54	dep	yield	563:567	arg1	increased					569:577	increased	569:577	yield increased 2 times to 94%	563:592	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	6	55	with	times	909:913	arg1	recovery					935:942	100% cellulose recovery	920:942	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	6	55	with	times	909:913	arg1	removal					964:970	29% lignin removal	953:970	100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3	920:995	EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.					
30927653	3	56	dep	rate	549:552	arg1	yield					563:567	yield	563:567	yield increased 2 times to 94%	563:592	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	5	57	theme	enzyme	777:782	arg1	ability					795:801	The enzyme adsorption ability	773:801	The enzyme adsorption ability of pretreated straw	773:821	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	5	57	theme	enzyme	777:782	arg1	that					836:839	that	836:839	that	836:839	The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample.					
30927653	2	58	dep	had	237:239	arg1	with					364:367	with	364:367	with	364:367	EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3.					
30927653	3	59	theme	cellulase	607:615	arg1	loading					617:623	cellulase loading	607:623	cellulase loading of 10 FPU/g	607:635	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
30927653	4	60	theme	substrates	715:724	arg1	structure					702:710	structure	702:710	structure	702:710	The hydrolysis behavior was correlated with the composition and structure of substrates characterized by SEM, FT-IR, BET, XRD and TGA.					
30927653	4	60	theme	substrates	715:724	arg1	composition					686:696	composition	686:696	composition	686:696	The hydrolysis behavior was correlated with the composition and structure of substrates characterized by SEM, FT-IR, BET, XRD and TGA.					
30927653	3	61	theme	pretreated	480:489	arg1	residue					491:497	The pretreated residue	476:497	The pretreated residue (5%, w/v)	476:507	The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g.					
29634785	8	0	theme	alkaline-treated	1244:1259	arg1	bagasse					1275:1281	alkaline-treated sweet sorghum bagasse	1244:1281	alkaline-treated sweet sorghum bagasse	1244:1281	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	9	1	theme	polymerization	1546:1559	arg1	degrees					1535:1541	average degrees	1527:1541	average degrees of polymerization of 11-12	1527:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	4	2	theme	sorghum	729:735	arg1	bagasse					737:743	the sweet sorghum bagasse	719:743	the sweet sorghum bagasse to achieve maximum hemicelluloses yield	719:783	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	9	3	theme	present	1350:1356	arg1	study					1358:1362	the present study	1346:1362	the present study	1346:1362	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	7	4	theme	processing	1139:1148	arg1	MR44					1133:1136	MR44	1133:1136	MR44	1133:1136	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	7	4	theme	processing	1139:1148	arg1	MeGAXn					1150:1155	processing MeGAXn	1139:1155	processing MeGAXn from sweet sorghum and accumulating U-XOS	1139:1197	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	9	5	theme	alkaline	1389:1396	arg1	treatment					1398:1406	direct alkaline treatment	1382:1406	direct alkaline treatment of sweet sorghum bagasse	1382:1431	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	3	6	theme	present	529:535	arg1	studies					537:543	The present studies	525:543	The present studies	525:543	The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS.					
29634785	0	7	from	Optimization	0:11	arg1	application					95:105	its direct application	84:105	its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	84:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	0	7	from	Optimization	0:11	arg1	bagasse					72:78	sweet sorghum bagasse	58:78	sweet sorghum bagasse	58:78	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	9	8	with	conversion	1502:1511	arg1	degrees					1535:1541	average degrees	1527:1541	average degrees of polymerization of 11-12	1527:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	7	9	from	sorghum	1168:1174	arg1	MR44					1133:1136	MR44	1133:1136	MR44	1133:1136	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	7	9	from	sorghum	1168:1174	arg1	MeGAXn					1150:1155	processing MeGAXn	1139:1155	processing MeGAXn from sweet sorghum and accumulating U-XOS	1139:1197	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	9	10	theme	MeGAXn	1477:1482	arg1	bioavailability					1458:1472	the bioavailability	1454:1472	the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12	1454:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	3	11	theme	direct	578:583	arg1	extraction					594:603	direct alkaline extraction	578:603	direct alkaline extraction serving for production of U-XOS	578:635	The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS.					
29634785	6	12	theme	xylose	996:1001	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	0	13	theme	direct	88:93	arg1	application					95:105	its direct application	84:105	its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	84:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	9	14	theme	average	1527:1533	arg1	degrees					1535:1541	average degrees	1527:1541	average degrees of polymerization of 11-12	1527:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	8	15	theme	sorghum	1267:1273	arg1	bagasse					1275:1281	alkaline-treated sweet sorghum bagasse	1244:1281	alkaline-treated sweet sorghum bagasse	1244:1281	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	7	16	theme	sweet	1162:1166	arg1	sorghum					1168:1174	sweet sorghum and accumulating U-XOS	1162:1197	sorghum	1168:1174	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	4	17	theme	sweet	723:727	arg1	bagasse					737:743	the sweet sorghum bagasse	719:743	the sweet sorghum bagasse to achieve maximum hemicelluloses yield	719:783	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	9	18	theme	bagasse	1425:1431	arg1	treatment					1398:1406	direct alkaline treatment	1382:1406	direct alkaline treatment of sweet sorghum bagasse	1382:1431	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	9	19	theme	MR44-mediated	1488:1500	arg1	conversion					1502:1511	MR44-mediated conversion	1488:1511	MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12	1488:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	6	20	theme	glucose	1030:1036	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	4	21	theme	xylan	688:692	arg1	conditions					705:714	xylan extraction conditions	688:714	xylan extraction conditions	688:714	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	2	22	theme	MeGAXn	496:501	arg1	biocatalyst					392:402	an engineered biocatalyst to secrete only the XynC xylanase	378:436	an engineered biocatalyst to secrete only the XynC xylanase	378:436	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	2	22	theme	MeGAXn	496:501	arg1	capable					474:480	capable	474:480	capable	474:480	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	7	23	from	U-XOS	1193:1197	arg1	MR44					1133:1136	MR44	1133:1136	MR44	1133:1136	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	7	23	from	U-XOS	1193:1197	arg1	MeGAXn					1150:1155	processing MeGAXn	1139:1155	processing MeGAXn from sweet sorghum and accumulating U-XOS	1139:1197	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	7	24	theme	xylan	1095:1099	arg1	preparation					1101:1111	this xylan preparation	1090:1111	this xylan preparation	1090:1111	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	5	25	dep	follows	817:823	arg1	time					837:840	extraction time	826:840	extraction time of 3.91 h	826:850	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	25	dep	follows	817:823	arg1	w/w					911:913	w/w	911:913	w/w	911:913	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	25	dep	follows	817:823	arg1	temperature					864:874	extraction temperature	853:874	extraction temperature of 86.1°C	853:884	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	25	dep	follows	817:823	arg1	concentration					896:908	NaOH concentration	891:908	NaOH concentration (w/w) of 12.33%	891:924	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	3	26	theme	alkaline	585:592	arg1	extraction					594:603	direct alkaline extraction	578:603	direct alkaline extraction serving for production of U-XOS	578:635	The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS.					
29634785	9	27	theme	human	1638:1642	arg1	medicine					1659:1666	human and veterinary medicine	1638:1666	human and veterinary medicine	1638:1666	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	5	28	theme	NaOH	891:894	arg1	w/w					911:913	w/w	911:913	w/w	911:913	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	28	theme	NaOH	891:894	arg1	concentration					896:908	NaOH concentration	891:908	NaOH concentration (w/w) of 12.33%	891:924	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	4	29	theme	hemicelluloses	764:777	arg1	yield					779:783	maximum hemicelluloses yield	756:783	maximum hemicelluloses yield	756:783	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	1	30	theme	predominant	194:204	arg1	components					206:215	predominant components	194:215	predominant components of hemicelluloses in grasses	194:244	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	0	31	theme	extraction	25:34	arg1	Optimization					0:11	Optimization	0:11	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.	0:189	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	6	32	theme	compositional	970:982	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	9	33	theme	veterinary	1648:1657	arg1	medicine					1659:1666	human and veterinary medicine	1638:1666	human and veterinary medicine	1638:1666	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	9	34	from	applications	1608:1619	arg1	medicine					1659:1666	human and veterinary medicine	1638:1666	human and veterinary medicine	1638:1666	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	9	34	from	applications	1608:1619	arg1	nutrition					1624:1632	nutrition	1624:1632	nutrition	1624:1632	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	5	35	theme	extraction	826:835	arg1	time					837:840	extraction time	826:840	extraction time of 3.91 h	826:850	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	36	theme	extraction	853:862	arg1	temperature					864:874	extraction temperature	853:874	extraction temperature of 86.1°C	853:884	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	0	37	theme	alkaline	16:23	arg1	extraction					25:34	alkaline extraction	16:34	alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	16:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	2	38	dep	Bacillus	354:361	arg1	subtilis					363:370	subtilis	363:370	subtilis	363:370	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	5	39	theme	h	850:850	arg1	time					837:840	extraction time	826:840	extraction time of 3.91 h	826:850	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	39	theme	h	850:850	arg1	w/w					911:913	w/w	911:913	w/w	911:913	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	39	theme	h	850:850	arg1	temperature					864:874	extraction temperature	853:874	extraction temperature of 86.1°C	853:884	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	39	theme	h	850:850	arg1	concentration					896:908	NaOH concentration	891:908	NaOH concentration (w/w) of 12.33%	891:924	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	0	40	theme	hemicellulose	39:51	arg1	extraction					25:34	alkaline extraction	16:34	alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	16:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	5	41	theme	%	924:924	arg1	time					837:840	extraction time	826:840	extraction time of 3.91 h	826:850	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	41	theme	%	924:924	arg1	w/w					911:913	w/w	911:913	w/w	911:913	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	41	theme	%	924:924	arg1	temperature					864:874	extraction temperature	853:874	extraction temperature of 86.1°C	853:884	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	41	theme	%	924:924	arg1	concentration					896:908	NaOH concentration	891:908	NaOH concentration (w/w) of 12.33%	891:924	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	0	42	theme	xylooligosaccharides	136:155	arg1	production					115:124	the production	111:124	the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	111:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	1	43	theme	hemicelluloses	220:233	arg1	components					206:215	predominant components	194:215	predominant components of hemicelluloses in grasses	194:244	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	4	44	theme	extraction	694:703	arg1	conditions					705:714	xylan extraction conditions	688:714	xylan extraction conditions	688:714	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	2	45	theme	XynC	424:427	arg1	xylanase					429:436	only the XynC xylanase	415:436	only the XynC xylanase	415:436	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	2	46	theme	processing	485:494	arg1	MeGAXn					496:501	processing MeGAXn	485:501	processing MeGAXn	485:501	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	1	47	theme	acidic	317:322	arg1	xylooligosaccharides					324:343	acidic xylooligosaccharides	317:343	acidic xylooligosaccharides (U-XOS)	317:351	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	1	47	theme	acidic	317:322	arg1	U-XOS					346:350	U-XOS	346:350	U-XOS	346:350	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	0	48	theme	acidic	129:134	arg1	xylooligosaccharides					136:155	acidic xylooligosaccharides	129:155	acidic xylooligosaccharides	129:155	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	0	49	from	bagasse	72:78	arg1	Optimization					0:11	Optimization	0:11	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.	0:189	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	0	49	from	bagasse	72:78	arg1	extraction					25:34	alkaline extraction	16:34	alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	16:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	2	50	theme	arabinoxylan	448:459	arg1	hydrolase					461:469	Axh43 arabinoxylan hydrolase	442:469	Axh43 arabinoxylan hydrolase	442:469	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	1	51	from	hemicelluloses	220:233	arg1	grasses					238:244	grasses	238:244	grasses	238:244	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	5	52	theme	86.1°C	879:884	arg1	time					837:840	extraction time	826:840	extraction time of 3.91 h	826:850	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	52	theme	86.1°C	879:884	arg1	w/w					911:913	w/w	911:913	w/w	911:913	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	52	theme	86.1°C	879:884	arg1	temperature					864:874	extraction temperature	853:874	extraction temperature of 86.1°C	853:884	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	5	52	theme	86.1°C	879:884	arg1	concentration					896:908	NaOH concentration	891:908	NaOH concentration (w/w) of 12.33%	891:924	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	0	53	theme	sweet	58:62	arg1	bagasse					72:78	sweet sorghum bagasse	58:78	sweet sorghum bagasse	58:78	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	1	54	theme	xylooligosaccharides	324:343	arg1	production					303:312	the production	299:312	the production of acidic xylooligosaccharides (U-XOS)	299:351	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	0	55	theme	Bacillus	160:167	arg1	strain					178:183	Bacillus subtilis strain MR44	160:188	Bacillus subtilis strain MR44	160:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	7	56	theme	MR44	1133:1136	arg1	growth					1123:1128	growth	1123:1128	growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS	1123:1197	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	9	57	theme	sweet	1411:1415	arg1	bagasse					1425:1431	sweet sorghum bagasse	1411:1431	sweet sorghum bagasse	1411:1431	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	6	58	theme	lignin	1046:1051	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	8	59	theme	U-XOS	1215:1219	arg1	comparable					1287:1296	comparable	1287:1296	comparable	1287:1296	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	8	59	theme	U-XOS	1215:1219	arg1	quality					1204:1210	The quality	1200:1210	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse	1200:1281	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	1	60	from	components	206:215	arg1	grasses					238:244	grasses	238:244	grasses	238:244	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	2	61	theme	Bacillus	354:361	arg1	MR44					372:375	Bacillus subtilis MR44	354:375	Bacillus subtilis MR44	354:375	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	9	62	theme	alternative	1581:1591	arg1	resources					1593:1601	alternative resources	1581:1601	alternative resources	1581:1601	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	1	63	from	grasses	238:244	arg1	components					206:215	predominant components	194:215	predominant components of hemicelluloses in grasses	194:244	As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS).					
29634785	9	64	theme	11-12	1564:1568	arg1	polymerization					1546:1559	polymerization	1546:1559	polymerization of 11-12	1546:1568	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	8	65	theme	purified	1320:1327	arg1	MeGAXn					1329:1334	purified MeGAXn	1320:1334	purified MeGAXn	1320:1334	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	4	66	theme	maximum	756:762	arg1	yield					779:783	maximum hemicelluloses yield	756:783	maximum hemicelluloses yield	756:783	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	6	67	theme	arabinose	1012:1020	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	0	68	theme	subtilis	169:176	arg1	strain					178:183	Bacillus subtilis strain MR44	160:188	Bacillus subtilis strain MR44	160:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	0	69	from	application	95:105	arg1	Optimization					0:11	Optimization	0:11	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.	0:189	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	0	69	from	application	95:105	arg1	extraction					25:34	alkaline extraction	16:34	alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44	16:188	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	4	70	used	used	671:674	arg2	Methodology					655:665	Response Surface Methodology	638:665	Response Surface Methodology	638:665	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	9	71	theme	direct	1382:1387	arg1	treatment					1398:1406	direct alkaline treatment	1382:1406	direct alkaline treatment of sweet sorghum bagasse	1382:1431	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	8	72	theme	sweet	1261:1265	arg1	bagasse					1275:1281	alkaline-treated sweet sorghum bagasse	1244:1281	alkaline-treated sweet sorghum bagasse	1244:1281	The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn.					
29634785	6	73	theme	ash	1057:1059	arg1	analysis					984:991	a compositional analysis	968:991	a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%)	968:1066	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	5	74	theme	optimized	790:798	arg1	condition					800:808	The optimized condition	786:808	The optimized condition	786:808	The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%.					
29634785	3	75	from	explosion	565:573	arg1	extraction					594:603	direct alkaline extraction	578:603	direct alkaline extraction serving for production of U-XOS	578:635	The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS.					
29634785	3	76	theme	U-XOS	631:635	arg1	production					617:626	production	617:626	production of U-XOS	617:635	The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS.					
29634785	2	77	theme	engineered	381:390	arg1	biocatalyst					392:402	an engineered biocatalyst to secrete only the XynC xylanase	378:436	an engineered biocatalyst to secrete only the XynC xylanase	378:436	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	2	77	theme	engineered	381:390	arg1	capable					474:480	capable	474:480	capable	474:480	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	9	78	theme	sorghum	1417:1423	arg1	bagasse					1425:1431	sweet sorghum bagasse	1411:1431	sweet sorghum bagasse	1411:1431	Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11-12, providing alternative resources with applications in nutrition and human and veterinary medicine.					
29634785	4	79	theme	Surface	647:653	arg1	Methodology					655:665	Response Surface Methodology	638:665	Response Surface Methodology	638:665	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
29634785	0	80	theme	sorghum	64:70	arg1	bagasse					72:78	sweet sorghum bagasse	58:78	sweet sorghum bagasse	58:78	Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44.					
29634785	6	81	theme	Crude	927:931	arg1	xylan					933:937	Crude xylan	927:937	Crude xylan extracted with NaOH	927:957	Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%).					
29634785	2	82	theme	Axh43	442:446	arg1	hydrolase					461:469	Axh43 arabinoxylan hydrolase	442:469	Axh43 arabinoxylan hydrolase	442:469	Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS.					
29634785	7	83	theme	accumulating	1180:1191	arg1	U-XOS					1193:1197	sweet sorghum and accumulating U-XOS	1162:1197	U-XOS	1193:1197	After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS.					
29634785	4	84	theme	Response	638:645	arg1	Methodology					655:665	Response Surface Methodology	638:665	Response Surface Methodology	638:665	Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield.					
30318234	3	0	theme	high	685:688	arg1	IC50					713:716	IC50	713:716	IC50 at 227 μg/mL	713:729	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	0	theme	high	685:688	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	0	theme	high	685:688	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	0	theme	high	685:688	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	1	theme	radical	799:805	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	1	theme	radical	799:805	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	2	theme	reducing	762:769	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	2	theme	reducing	762:769	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	3	with	comparable	732:741	arg1	BHT					748:750	BHT	748:750	BHT	748:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	4	theme	scavenging	807:816	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	4	theme	scavenging	807:816	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	4	5	theme	potential	914:922	arg1	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	5	theme	potential	914:922	arg1	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	3	6	theme	better	668:673	arg1	activity					659:666	excellent superoxide radical activity	630:666	excellent superoxide radical activity better than BHT	630:682	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	7	theme	MDPs	522:525	arg1	activities					508:517	the antioxidant activities	492:517	the antioxidant activities of MDPs	492:525	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	8	theme	hydroxyl	790:797	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	8	theme	hydroxyl	790:797	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	9	theme	antioxidant	496:506	arg1	activities					508:517	the antioxidant activities	492:517	the antioxidant activities of MDPs	492:525	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	2	10	theme	mannose	384:390	arg1	acid					439:442	galacturonic acid	426:442	galacturonic acid	426:442	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	10	theme	mannose	384:390	arg1	rhamnose					459:466	rhamnose	459:466	rhamnose	459:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	10	theme	mannose	384:390	arg1	compositions					368:379	the mainly compositions	357:379	the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose	357:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	10	theme	mannose	384:390	arg1	galactose					445:453	galactose	445:453	galactose	445:453	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	4	11	theme	natural	924:930	arg1	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	11	theme	natural	924:930	arg1	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	3	12	theme	DPPH	690:693	arg1	IC50					713:716	IC50	713:716	IC50 at 227 μg/mL	713:729	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	12	theme	DPPH	690:693	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	12	theme	DPPH	690:693	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	12	theme	DPPH	690:693	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	4	13	used	used	904:907	arg2	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	13	used	used	904:907	arg2	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	3	14	theme	radical	695:701	arg1	IC50					713:716	IC50	713:716	IC50 at 227 μg/mL	713:729	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	14	theme	radical	695:701	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	14	theme	radical	695:701	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	14	theme	radical	695:701	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	15	from	227 μg/mL	721:729	arg1	IC50					713:716	IC50	713:716	IC50 at 227 μg/mL	713:729	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	15	from	227 μg/mL	721:729	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	1	16	theme	liquid	192:197	arg1	fermentation					179:190	the fermentation liquid	175:197	the fermentation liquid of M. dendrobii	175:213	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
30318234	2	17	theme	GC	318:319	arg1	spectra					337:343	FT-IR, GC, 1H and 13C NMR spectra	311:343	spectra	337:343	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	1	18	theme	M.	202:203	arg1	dendrobii					205:213	M. dendrobii	202:213	M. dendrobii	202:213	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
30318234	2	19	theme	galactose	405:413	arg1	acid					439:442	galacturonic acid	426:442	galacturonic acid	426:442	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	19	theme	galactose	405:413	arg1	rhamnose					459:466	rhamnose	459:466	rhamnose	459:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	19	theme	galactose	405:413	arg1	compositions					368:379	the mainly compositions	357:379	the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose	357:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	19	theme	galactose	405:413	arg1	galactose					445:453	galactose	445:453	galactose	445:453	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	20	theme	FT-IR	311:315	arg1	spectra					337:343	FT-IR, GC, 1H and 13C NMR spectra	311:343	spectra	337:343	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	1	21	theme	dendrobii	205:213	arg1	fermentation					179:190	the fermentation liquid	175:197	the fermentation liquid of M. dendrobii	175:213	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
30318234	0	22	theme	antioxidant	44:54	arg1	activities					56:65	antioxidant activities	44:65	antioxidant activities	44:65	Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii.					
30318234	3	23	theme	excellent	630:638	arg1	activity					659:666	excellent superoxide radical activity	630:666	excellent superoxide radical activity better than BHT	630:682	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	2	24	theme	xylose	393:398	arg1	acid					439:442	galacturonic acid	426:442	galacturonic acid	426:442	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	24	theme	xylose	393:398	arg1	rhamnose					459:466	rhamnose	459:466	rhamnose	459:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	24	theme	xylose	393:398	arg1	compositions					368:379	the mainly compositions	357:379	the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose	357:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	24	theme	xylose	393:398	arg1	galactose					445:453	galactose	445:453	galactose	445:453	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	1	25	theme	anion	218:222	arg1	chromatography					233:246	anion exchange chromatography	218:246	anion exchange chromatography	218:246	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
30318234	3	26	theme	different	554:562	arg1	activities					576:585	different antioxidant activities	554:585	different antioxidant activities	554:585	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	2	27	theme	NMR	333:335	arg1	spectra					337:343	FT-IR, GC, 1H and 13C NMR spectra	311:343	spectra	337:343	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	28	theme	galacturonic	426:437	arg1	acid					439:442	galacturonic acid	426:442	galacturonic acid	426:442	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	28	theme	galacturonic	426:437	arg1	compositions					368:379	the mainly compositions	357:379	the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose	357:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	3	29	theme	comparable	732:741	arg1	IC50					713:716	IC50	713:716	IC50 at 227 μg/mL	713:729	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	29	theme	comparable	732:741	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	29	theme	comparable	732:741	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	29	theme	comparable	732:741	arg1	activity					818:825	hydroxyl radical scavenging activity	790:825	hydroxyl radical scavenging activity	790:825	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	4	30	theme	liquid	872:877	arg1	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	30	theme	liquid	872:877	arg1	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	3	31	theme	antioxidant	564:574	arg1	activities					576:585	different antioxidant activities	554:585	different antioxidant activities	554:585	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	2	32	theme	13C	329:331	arg1	spectra					337:343	FT-IR, GC, 1H and 13C NMR spectra	311:343	spectra	337:343	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	4	33	theme	dendrobii	885:893	arg1	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	33	theme	dendrobii	885:893	arg1	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	2	34	dep	compositions	368:379	arg1	acid					439:442	galacturonic acid	426:442	galacturonic acid	426:442	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	34	dep	compositions	368:379	arg1	compositions					368:379	the mainly compositions	357:379	the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose	357:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	34	dep	compositions	368:379	arg1	rhamnose					459:466	rhamnose	459:466	rhamnose	459:466	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	34	dep	compositions	368:379	arg1	galactose					445:453	galactose	445:453	galactose	445:453	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	2	35	theme	chemical	274:281	arg1	structures					283:292	Their chemical structures	268:292	Their chemical structures	268:292	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	1	36	theme	exchange	224:231	arg1	chromatography					233:246	anion exchange chromatography	218:246	anion exchange chromatography	218:246	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
30318234	3	37	theme	superoxide	640:649	arg1	activity					659:666	excellent superoxide radical activity	630:666	excellent superoxide radical activity better than BHT	630:682	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	2	38	theme	1H	322:323	arg1	spectra					337:343	FT-IR, GC, 1H and 13C NMR spectra	311:343	spectra	337:343	Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2.					
30318234	4	39	theme	antioxidant	942:952	arg1	source					932:937	a potential natural source	912:937	a potential natural source of antioxidant	912:952	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	4	39	theme	antioxidant	942:952	arg1	fermentation					859:870	the fermentation liquid	855:877	the fermentation liquid of M. dendrobii	855:893	The results indicated that the fermentation liquid of M. dendrobii could be used as a potential natural source of antioxidant.					
30318234	3	40	theme	radical	651:657	arg1	activity					659:666	excellent superoxide radical activity	630:666	excellent superoxide radical activity better than BHT	630:682	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	41	theme	moderate	753:760	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	41	theme	moderate	753:760	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	0	42	theme	polysaccharides	70:84	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii.					
30318234	0	42	theme	polysaccharides	70:84	arg1	characterization					23:38	chemical characterization	14:38	chemical characterization	14:38	Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii.					
30318234	0	42	theme	polysaccharides	70:84	arg1	activities					56:65	antioxidant activities	44:65	antioxidant activities	44:65	Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii.					
30318234	0	43	theme	chemical	14:21	arg1	characterization					23:38	chemical characterization	14:38	chemical characterization	14:38	Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii.					
30318234	3	44	theme	power	771:775	arg1	activity					777:784	moderate reducing power activity	753:784	moderate reducing power activity	753:784	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	3	44	theme	power	771:775	arg1	activity					703:710	high DPPH radical activity	685:710	high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT	685:750	Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP-2 performed noticeable, with excellent superoxide radical activity better than BHT, high DPPH radical activity (IC50 at 227 μg/mL) comparable with BHT, moderate reducing power activity and hydroxyl radical scavenging activity.					
30318234	1	45	theme	gel	252:254	arg1	filtration					256:265	gel filtration	252:265	gel filtration	252:265	Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration.					
29689470	3	0	theme	oligosaccharide	677:691	arg1	composition					693:703	oligosaccharide composition	677:703	oligosaccharide composition	677:703	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	5	1	dep	depth	960:964	arg1	features					981:988	the structural features	966:988	depth the structural features of such a drug	960:1003	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	3	2	from	investigation	482:494	arg1	features					514:521	the structural features	499:521	the structural features of both the whole mixture and the isolated components	499:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	3	3	theme	di	670:671	arg1	analysis					609:616	the analysis	605:616	the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition	605:703	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	4	4	theme	detailed	868:875	arg1	analysis					888:895	detailed structural analysis	868:895	detailed structural analysis	868:895	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	4	5	theme	affinity	812:819	arg1	endowed					794:800	endowed	794:800	endowed	794:800	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	1	6	used	used	159:162	arg2	Sulodexide					48:57	Sulodexide	48:57	Sulodexide	48:57	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	6	used	used	159:162	arg2	heparinoid					64:73	a heparinoid	62:73	a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy	62:283	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	3	7	theme	mixture	541:547	arg1	features					514:521	the structural features	499:521	the structural features of both the whole mixture and the isolated components	499:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	3	8	theme	HP-SEC/TDA	708:717	arg1	techniques					739:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	5	9	theme	techniques	927:936	arg1	combination					902:912	The combination	898:912	The combination of different techniques	898:936	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	1	10	dep	components	112:121	arg1	components					112:121	its components	108:121	its components heparin and dermatan sulfate	108:150	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	10	dep	components	112:121	arg1	heparin					123:129	heparin	123:129	heparin	123:129	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	10	dep	components	112:121	arg1	sulfate					144:150	dermatan sulfate	135:150	dermatan sulfate	135:150	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	3	11	theme	composition	693:703	arg1	analysis					609:616	the analysis	605:616	the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition	605:703	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	4	12	theme	fractions	784:792	arg1	separation					770:779	also the separation	761:779	also the separation of fractions endowed of graded affinity to antithrombin	761:835	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	0	13	theme	structural	5:14	arg1	characterization					16:31	Fine structural characterization	0:31	Fine structural characterization of sulodexide.	0:46	Fine structural characterization of sulodexide.					
29689470	3	14	theme	HPLC-MS	731:737	arg1	techniques					739:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	0	15	theme	Fine	0:3	arg1	characterization					16:31	Fine structural characterization	0:31	Fine structural characterization of sulodexide.	0:46	Fine structural characterization of sulodexide.					
29689470	1	16	theme	diabetic	264:271	arg1	nephropathy					273:283	diabetic nephropathy	264:283	diabetic nephropathy	264:283	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	5	17	theme	batch	1055:1059	arg1	production					1061:1070	batch production	1055:1070	batch production	1055:1070	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	1	18	theme	nephropathy	273:283	arg1	treatment					251:259	the treatment	247:259	the treatment of diabetic nephropathy	247:283	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	4	19	theme	graded	805:810	arg1	affinity					812:819	graded affinity	805:819	graded affinity to antithrombin	805:835	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	2	20	theme	clinical	299:306	arg1	studies					308:314	many clinical studies	294:314	many clinical studies	294:314	Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components.					
29689470	2	21	theme	fine	405:408	arg1	characterization					419:434	the fine chemical characterization	401:434	the fine chemical characterization of its components	401:452	Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components.					
29689470	2	22	theme	chemical	410:417	arg1	characterization					419:434	the fine chemical characterization	401:434	the fine chemical characterization of its components	401:452	Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components.					
29689470	3	23	theme	2D-NMR	720:725	arg1	techniques					739:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	HP-SEC/TDA, 2D-NMR and HPLC-MS techniques	708:748	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	5	24	theme	structural	970:979	arg1	features					981:988	the structural features	966:988	depth the structural features of such a drug	960:1003	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	3	25	theme	weight	631:636	arg1	distribution					638:649	molecular weight distribution	621:649	molecular weight distribution	621:649	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	2	26	theme	many	294:297	arg1	studies					308:314	many clinical studies	294:314	many clinical studies	294:314	Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components.					
29689470	3	27	theme	distribution	638:649	arg1	analysis					609:616	the analysis	605:616	the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition	605:703	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	0	28	theme	sulodexide	36:45	arg1	characterization					16:31	Fine structural characterization	0:31	Fine structural characterization of sulodexide.	0:46	Fine structural characterization of sulodexide.					
29689470	5	29	theme	production	1061:1070	arg1	analysis					1043:1050	possible analysis	1034:1050	possible analysis of batch production	1034:1070	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	4	30	theme	endowed	794:800	arg1	fractions					784:792	fractions	784:792	fractions endowed of graded affinity to antithrombin	784:835	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	3	31	theme	molecular	621:629	arg1	distribution					638:649	molecular weight distribution	621:649	molecular weight distribution	621:649	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	3	32	theme	isolated	557:564	arg1	components					566:575	the isolated components	553:575	the isolated components	553:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	1	33	theme	components	112:121	arg1	properties					94:103	the properties	90:103	the properties of its components heparin and dermatan sulfate	90:150	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	2	34	theme	components	443:452	arg1	characterization					419:434	the fine chemical characterization	401:434	the fine chemical characterization of its components	401:452	Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components.					
29689470	4	35	theme	structural	877:886	arg1	analysis					888:895	detailed structural analysis	868:895	detailed structural analysis	868:895	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	5	36	theme	useful	1018:1023	arg1	tool					1025:1028	a useful tool	1016:1028	a useful tool for possible analysis of batch production	1016:1070	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	3	37	theme	components	566:575	arg1	features					514:521	the structural features	499:521	the structural features of both the whole mixture and the isolated components	499:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	3	38	theme	in-depth	473:480	arg1	investigation					482:494	the in-depth investigation	469:494	the in-depth investigation on the structural features of both the whole mixture and the isolated components	469:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	3	39	theme	mono	664:667	arg1	analysis					609:616	the analysis	605:616	the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition	605:703	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	5	40	theme	different	917:925	arg1	techniques					927:936	different techniques	917:936	different techniques	917:936	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	3	41	theme	whole	535:539	arg1	mixture					541:547	the whole mixture	531:547	the whole mixture	531:547	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29689470	1	42	theme	thromboembolic	210:223	arg1	diseases					225:232	thromboembolic diseases	210:232	thromboembolic diseases	210:232	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	5	43	theme	possible	1034:1041	arg1	analysis					1043:1050	possible analysis	1034:1050	possible analysis of batch production	1034:1070	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	1	44	theme	dermatan	135:142	arg1	components					112:121	its components	108:121	its components heparin and dermatan sulfate	108:150	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	44	theme	dermatan	135:142	arg1	sulfate					144:150	dermatan sulfate	135:150	dermatan sulfate	135:150	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	45	theme	diseases	225:232	arg1	prophylaxis					181:191	prophylaxis	181:191	prophylaxis	181:191	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	1	46	dep	prophylaxis	181:191	arg1	the					177:179	the	177:179	the	177:179	Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy.					
29689470	4	47	dep	achieved	841:848	arg1	followed					850:857	followed	850:857	followed again by detailed structural analysis	850:895	Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis.					
29689470	5	48	theme	drug	1000:1003	arg1	features					981:988	the structural features	966:988	depth the structural features of such a drug	960:1003	The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production.					
29689470	3	49	theme	structural	503:512	arg1	features					514:521	the structural features	499:521	the structural features of both the whole mixture and the isolated components	499:575	In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques.					
29449697	0	0	theme	plant	100:104	arg1	tissues					106:112	plant tissues	100:112	plant tissues	100:112	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	4	1	theme	exceptional	587:597	arg1	specificity					599:609	an exceptional specificity	584:609	an exceptional specificity for β-linked glucans	584:630	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	3	2	dep	structure-responsive	415:434	arg1	heptameric					437:446	heptameric	437:446	heptameric	437:446	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	5	3	theme	optical	638:644	arg1	method					662:667	This optical, non-disruptive method	633:667	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages	633:725	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	9	4	theme	standard	1613:1620	arg1	operations					1622:1631	standard operations	1613:1631	standard operations	1613:1631	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	3	5	theme	optical	361:367	arg1	method					369:374	an optical method	358:374	an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore	358:493	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	4	6	theme	β-linked	615:622	arg1	glucans					624:630	β-linked glucans	615:630	β-linked glucans	615:630	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	5	7	theme	linkages	718:725	arg1	differentiation					688:702	stereochemical differentiation	673:702	stereochemical differentiation of glycosidic linkages	673:725	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	1	8	theme	non-disruptive	191:204	arg1	analysis					218:225	non-disruptive composition analysis	191:225	non-disruptive composition analysis	191:225	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	8	9	theme	chemical	1349:1356	arg1	constituents					1358:1369	the chemical constituents	1345:1369	the chemical constituents in plant tissues	1345:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	7	10	theme	annular	1151:1157	arg1	thickenings					1159:1169	lignified annular thickenings	1141:1169	lignified annular thickenings of the trachea	1141:1184	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	6	11	theme	cellulose	871:879	arg1	features					906:913	structural cell wall features	885:913	structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem	885:977	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	11	theme	cellulose	871:879	arg1	plasmodesmata					923:935	plasmodesmata	923:935	plasmodesmata	923:935	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	11	theme	cellulose	871:879	arg1	localization					855:866	spatial localization	847:866	spatial localization of cellulose	847:879	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	11	theme	cellulose	871:879	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	9	12	theme	biorefinery	1493:1503	arg1	industry					1505:1512	the biorefinery industry	1489:1512	the biorefinery industry	1489:1512	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	7	13	theme	lignified	1141:1149	arg1	thickenings					1159:1169	lignified annular thickenings	1141:1169	lignified annular thickenings of the trachea	1141:1184	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	14	theme	components	1030:1039	arg1	imaging					993:999	Simultaneous imaging	980:999	Simultaneous imaging of intrinsically fluorescent components	980:1039	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	15	theme	fluorescent	1018:1028	arg1	components					1030:1039	intrinsically fluorescent components	1004:1039	intrinsically fluorescent components	1004:1039	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	3	16	theme	cellulose	380:388	arg1	identification					390:403	cellulose identification	380:403	cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore	380:493	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	4	17	theme	related	559:565	arg1	glucans					567:573	closely related glucans	551:573	closely related glucans	551:573	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	8	18	theme	non-destructive	1233:1247	arg1	method					1249:1254	Our non-destructive method	1229:1254	Our non-destructive method for cellulose identification	1229:1283	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	1	19	theme	composition	206:216	arg1	analysis					218:225	non-disruptive composition analysis	191:225	non-disruptive composition analysis	191:225	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	8	20	theme	constituents	1358:1369	arg1	maps					1337:1340	anatomical maps	1326:1340	anatomical maps of the chemical constituents in plant tissues	1326:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	5	21	theme	in	745:746	arg1	analysis					765:772	in situ composition analysis	745:772	in situ composition analysis in plants	745:782	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	5	22	theme	stereochemical	673:686	arg1	differentiation					688:702	stereochemical differentiation	673:702	stereochemical differentiation of glycosidic linkages	673:725	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	0	23	theme	Stereochemical	0:13	arg1	identification					15:28	Stereochemical identification	0:28	Stereochemical identification of glucans by oligothiophenes	0:58	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	6	24	theme	spatial	847:853	arg1	localization					855:866	spatial localization	847:866	spatial localization of cellulose	847:879	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	9	25	theme	in-line	1564:1570	arg1	monitoring					1572:1581	in-line monitoring	1564:1581	feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations	1530:1631	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	9	26	theme	rapid	1394:1398	arg1	method					1414:1419	This rapid and versatile method	1389:1419	This rapid and versatile method	1389:1419	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	8	27	theme	maps	1337:1340	arg1	emergence					1313:1321	the emergence	1309:1321	the emergence of anatomical maps of the chemical constituents in plant tissues	1309:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	6	28	theme	perforated	941:950	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	2	29	from	polysaccharides	273:287	arg1	tissues					305:311	native plant tissues	292:311	native plant tissues	292:311	The ability to identify and spatially locate polysaccharides in native plant tissues is difficult but essential.					
29449697	4	30	with	interacting	534:544	arg1	glucans					567:573	closely related glucans	551:573	closely related glucans	551:573	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	3	31	theme	oligothiophene	448:461	arg1	h-FTAA					463:468	the structure-responsive, heptameric oligothiophene h-FTAA	411:468	the structure-responsive, heptameric oligothiophene h-FTAA	411:468	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	4	32	link	β-linked	615:622	arg1	glucans					624:630	β-linked glucans	615:630	β-linked glucans	615:630	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	5	33	theme	composition	753:763	arg1	analysis					765:772	in situ composition analysis	745:772	in situ composition analysis in plants	745:782	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	9	34	theme	versatile	1404:1412	arg1	method					1414:1419	This rapid and versatile method	1389:1419	This rapid and versatile method	1389:1419	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	9	35	theme	plant	1445:1449	arg1	fields					1468:1473	the plant science research fields	1441:1473	the plant science research fields	1441:1473	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	5	36	used	used	736:739	arg2	method					662:667	This optical, non-disruptive method	633:667	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages	633:725	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	8	37	from	maps	1337:1340	arg1	tissues					1380:1386	plant tissues	1374:1386	plant tissues	1374:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	0	38	theme	glucans	33:39	arg1	identification					15:28	Stereochemical identification	0:28	Stereochemical identification of glucans by oligothiophenes	0:58	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	9	39	theme	feedstock	1530:1538	arg1	optimization					1540:1551	feedstock optimization	1530:1551	feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations	1530:1631	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	8	40	from	tissues	1380:1386	arg1	maps					1337:1340	anatomical maps	1326:1340	anatomical maps of the chemical constituents in plant tissues	1326:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	5	41	theme	glycosidic	707:716	arg1	linkages					718:725	glycosidic linkages	707:725	glycosidic linkages	707:725	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	1	42	theme	Efficient	115:123	arg1	use					125:127	Efficient use	115:127	Efficient use of plant-derived materials	115:154	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	5	43	dep	optical	638:644	arg1	non-disruptive					647:660	non-disruptive	647:660	non-disruptive	647:660	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	7	44	theme	trachea	1178:1184	arg1	thickenings					1159:1169	lignified annular thickenings	1141:1169	lignified annular thickenings of the trachea	1141:1184	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	44	theme	trachea	1178:1184	arg1	chloroplasts					1124:1135	chloroplasts	1124:1135	chloroplasts	1124:1135	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	8	45	theme	cellulose	1260:1268	arg1	identification					1270:1283	cellulose identification	1260:1283	cellulose identification	1260:1283	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	4	46	theme	interacting	534:544	arg1	analysis					515:522	Spectrophotometric analysis	496:522	Spectrophotometric analysis of h-FTAA interacting with closely related glucans	496:573	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	7	47	theme	sub-cellular	1209:1220	arg1	scale					1222:1226	the sub-cellular scale	1205:1226	the sub-cellular scale	1205:1226	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	3	48	theme	molecular	473:481	arg1	fluorophore					483:493	molecular fluorophore	473:493	molecular fluorophore	473:493	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	4	49	theme	h-FTAA	527:532	arg1	interacting					534:544	h-FTAA interacting	527:544	h-FTAA interacting with closely related glucans	527:573	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	6	50	theme	Multi-laser/multi-detector	785:810	arg1	analysis					812:819	Multi-laser/multi-detector analysis	785:819	Multi-laser/multi-detector analysis developed herein	785:836	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	9	51	theme	reactions	1596:1604	arg1	optimization					1540:1551	feedstock optimization	1530:1551	feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations	1530:1631	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	9	51	theme	reactions	1596:1604	arg1	monitoring					1572:1581	in-line monitoring	1564:1581	feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations	1530:1631	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	0	52	from	mapping	89:95	arg1	tissues					106:112	plant tissues	100:112	plant tissues	100:112	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	3	53	theme	structure-responsive	415:434	arg1	h-FTAA					463:468	the structure-responsive, heptameric oligothiophene h-FTAA	411:468	the structure-responsive, heptameric oligothiophene h-FTAA	411:468	Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore.					
29449697	0	54	theme	cellulose	68:76	arg1	mapping					89:95	cellulose anatomical mapping	68:95	cellulose anatomical mapping in plant tissues	68:112	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	5	55	dep	in	745:746	arg1	situ					748:751	situ	748:751	situ	748:751	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	5	56	from	analysis	765:772	arg1	plants					777:782	plants	777:782	plants	777:782	This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants.					
29449697	4	57	theme	Spectrophotometric	496:513	arg1	analysis					515:522	Spectrophotometric analysis	496:522	Spectrophotometric analysis of h-FTAA interacting with closely related glucans	496:573	Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for β-linked glucans.					
29449697	8	58	from	constituents	1358:1369	arg1	tissues					1380:1386	plant tissues	1374:1386	plant tissues	1374:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	1	59	theme	plant-derived	132:144	arg1	materials					146:154	plant-derived materials	132:154	plant-derived materials	132:154	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	6	60	theme	sieve	952:956	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	61	theme	phloem	972:977	arg1	plasmodesmata					923:935	plasmodesmata	923:935	plasmodesmata	923:935	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	61	theme	phloem	972:977	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	62	theme	wall	901:904	arg1	features					906:913	structural cell wall features	885:913	structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem	885:977	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	62	theme	wall	901:904	arg1	plasmodesmata					923:935	plasmodesmata	923:935	plasmodesmata	923:935	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	62	theme	wall	901:904	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	1	63	theme	materials	146:154	arg1	use					125:127	Efficient use	115:127	Efficient use of plant-derived materials	115:154	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	7	64	theme	other	1098:1102	arg1	organelles					1104:1113	other organelles	1098:1113	other organelles	1098:1113	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	64	theme	other	1098:1102	arg1	thickenings					1159:1169	lignified annular thickenings	1141:1169	lignified annular thickenings of the trachea	1141:1184	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	64	theme	other	1098:1102	arg1	chloroplasts					1124:1135	chloroplasts	1124:1135	chloroplasts	1124:1135	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	1	65	link	plant-derived	132:144	arg1	materials					146:154	plant-derived materials	132:154	plant-derived materials	132:154	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	9	66	theme	research	1459:1466	arg1	fields					1468:1473	the plant science research fields	1441:1473	the plant science research fields	1441:1473	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	8	67	theme	plant	1374:1378	arg1	tissues					1380:1386	plant tissues	1374:1386	plant tissues	1374:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	6	68	theme	cell	896:899	arg1	features					906:913	structural cell wall features	885:913	structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem	885:977	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	68	theme	cell	896:899	arg1	plasmodesmata					923:935	plasmodesmata	923:935	plasmodesmata	923:935	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	68	theme	cell	896:899	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	7	69	theme	cell	1083:1086	arg1	walls					1088:1092	cell walls	1083:1092	cell walls	1083:1092	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	69	theme	cell	1083:1086	arg1	thickenings					1159:1169	lignified annular thickenings	1141:1169	lignified annular thickenings of the trachea	1141:1184	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	7	69	theme	cell	1083:1086	arg1	chloroplasts					1124:1135	chloroplasts	1124:1135	chloroplasts	1124:1135	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	9	70	theme	cellulose	1586:1594	arg1	reactions					1596:1604	cellulose reactions	1586:1604	cellulose reactions	1586:1604	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	8	71	theme	anatomical	1326:1335	arg1	maps					1337:1340	anatomical maps	1326:1340	anatomical maps of the chemical constituents in plant tissues	1326:1386	Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues.					
29449697	7	72	theme	spatial	1054:1060	arg1	relationship					1062:1073	the spatial relationship	1050:1073	the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision	1050:1200	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	2	73	theme	plant	299:303	arg1	tissues					305:311	native plant tissues	292:311	native plant tissues	292:311	The ability to identify and spatially locate polysaccharides in native plant tissues is difficult but essential.					
29449697	6	74	theme	structural	885:894	arg1	features					906:913	structural cell wall features	885:913	structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem	885:977	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	74	theme	structural	885:894	arg1	plasmodesmata					923:935	plasmodesmata	923:935	plasmodesmata	923:935	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	6	74	theme	structural	885:894	arg1	plates					958:963	perforated sieve plates	941:963	perforated sieve plates	941:963	Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem.					
29449697	1	75	theme	enabling	165:172	arg1	technologies					174:185	enabling technologies	165:185	enabling technologies	165:185	Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis.					
29449697	9	76	theme	science	1451:1457	arg1	fields					1468:1473	the plant science research fields	1441:1473	the plant science research fields	1441:1473	This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.					
29449697	7	77	with	relationship	1062:1073	arg1	precision					1192:1200	precision	1192:1200	precision	1192:1200	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
29449697	0	78	theme	anatomical	78:87	arg1	mapping					89:95	cellulose anatomical mapping	68:95	cellulose anatomical mapping in plant tissues	68:112	Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.					
29449697	2	79	theme	native	292:297	arg1	tissues					305:311	native plant tissues	292:311	native plant tissues	292:311	The ability to identify and spatially locate polysaccharides in native plant tissues is difficult but essential.					
29449697	7	80	theme	Simultaneous	980:991	arg1	imaging					993:999	Simultaneous imaging	980:999	Simultaneous imaging of intrinsically fluorescent components	980:1039	Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale.					
31726165	0	0	theme	polysaccharides	72:86	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	0	theme	polysaccharides	72:86	arg1	properties					48:57	rheological, thermal and antioxidant properties	11:57	rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits	11:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	4	1	contain	had	681:683	arg1	fractions					671:679	All pectic fractions	660:679	All pectic fractions	660:679	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	4	1	contain	had	681:683	arg2	degree					691:696	a low degree	685:696	a low degree of esterification (31.7-42.4%)	685:727	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	4	1	contain	had	681:683	arg2	%					726:726	31.7-42.4%	717:726	31.7-42.4%	717:726	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	5	2	theme	WSP	730:732	arg1	fraction					734:741	WSP fraction	730:741	WSP fraction	730:741	WSP fraction had the highest thermal stability among the five fractions.					
31726165	8	3	theme	1,4-β-D-Xylp	1062:1073	arg1	backbone					1075:1082	1,4-β-D-Xylp backbone	1062:1082	1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units	1062:1113	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	1	4	theme	structural	151:160	arg1	characteristics					162:176	structural characteristics	151:176	structural characteristics	151:176	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	0	5	theme	wall	67:70	arg1	polysaccharides					72:86	cell wall polysaccharides	62:86	cell wall polysaccharides from Chinese quince fruits	62:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	1	6	theme	sodium	330:335	arg1	NSP					363:365	NSP	363:365	NSP	363:365	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	6	theme	sodium	330:335	arg1	pectin					355:360	sodium carbonate-soluble pectin	330:360	sodium carbonate-soluble pectin (NSP)	330:366	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	6	theme	sodium	330:335	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	3	7	theme	wall	617:620	arg1	fraction					637:644	the predominant cell wall polysaccharide fraction	596:644	the predominant cell wall polysaccharide fraction in the fruit	596:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	9	8	theme	scavenging	1175:1184	arg1	activity					1186:1193	the highest DPPH radical scavenging activity	1150:1193	the highest DPPH radical scavenging activity	1150:1193	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	0	9	theme	Chinese	93:99	arg1	fruits					108:113	Chinese quince fruits	93:113	Chinese quince fruits	93:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	1	10	theme	carbonate-soluble	337:353	arg1	NSP					363:365	NSP	363:365	NSP	363:365	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	10	theme	carbonate-soluble	337:353	arg1	pectin					355:360	sodium carbonate-soluble pectin	330:360	sodium carbonate-soluble pectin (NSP)	330:366	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	10	theme	carbonate-soluble	337:353	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	8	11	theme	RG-I	1000:1003	arg1	chains					1032:1037	RG-I and galacturonic acid main chains	1000:1037	RG-I and galacturonic acid main chains	1000:1037	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	1	12	theme	4 mol/L	415:421	arg1	KSH-2					450:454	KSH-2	450:454	KSH-2	450:454	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	12	theme	4 mol/L	415:421	arg1	hemicellulose					435:447	4 mol/L KOH soluble hemicellulose	415:447	4 mol/L KOH soluble hemicellulose (KSH-2)	415:455	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	12	theme	4 mol/L	415:421	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	8	13	attach	attached	1084:1091	arg2	backbone					1075:1082	1,4-β-D-Xylp backbone	1062:1082	1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units	1062:1113	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	8	13	attach	attached	1084:1091	arg1	units					1109:1113	1,5-α-L-Araf units	1096:1113	1,5-α-L-Araf units	1096:1113	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	9	14	contain	has	1207:1209	arg2	power					1232:1236	the highest reducing power	1211:1236	the highest reducing power	1211:1236	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	9	14	contain	has	1207:1209	arg1	KSH-1					1201:1205	KSH-1	1201:1205	KSH-1	1201:1205	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	1	15	theme	water-soluble	271:283	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	15	theme	water-soluble	271:283	arg1	WSP					293:295	WSP	293:295	WSP	293:295	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	15	theme	water-soluble	271:283	arg1	pectin					285:290	water-soluble pectin	271:290	water-soluble pectin (WSP)	271:296	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	10	16	theme	food	1328:1331	arg1	industries					1352:1361	food and pharmaceutical industries	1328:1361	food and pharmaceutical industries	1328:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	0	17	from	properties	48:57	arg1	fruits					108:113	Chinese quince fruits	93:113	Chinese quince fruits	93:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	1	18	theme	KOH	423:425	arg1	KSH-2					450:454	KSH-2	450:454	KSH-2	450:454	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	18	theme	KOH	423:425	arg1	hemicellulose					435:447	4 mol/L KOH soluble hemicellulose	415:447	4 mol/L KOH soluble hemicellulose (KSH-2)	415:455	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	18	theme	KOH	423:425	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	7	19	contain	had	873:875	arg2	weight					899:904	molecular weight	889:904	the highest molecular weight	877:904	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	7	19	contain	had	873:875	arg1	CSP					869:871	CSP	869:871	CSP	869:871	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	1	20	theme	soluble	427:433	arg1	KSH-2					450:454	KSH-2	450:454	KSH-2	450:454	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	20	theme	soluble	427:433	arg1	hemicellulose					435:447	4 mol/L KOH soluble hemicellulose	415:447	4 mol/L KOH soluble hemicellulose (KSH-2)	415:455	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	20	theme	soluble	427:433	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	10	21	theme	polysaccharides	1309:1323	arg1	applications					1272:1283	the applications	1268:1283	the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries	1268:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	0	22	theme	quince	101:106	arg1	fruits					108:113	Chinese quince fruits	93:113	Chinese quince fruits	93:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	9	23	theme	reducing	1223:1230	arg1	power					1232:1236	the highest reducing power	1211:1236	the highest reducing power	1211:1236	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	6	24	theme	chain	822:826	arg1	lengths					828:834	The polysaccharide chain lengths	803:834	The polysaccharide chain lengths	803:834	The polysaccharide chain lengths ranged from 19.4 nm to 121.4 nm.					
31726165	10	25	theme	fruit	1303:1307	arg1	polysaccharides					1309:1323	Chinese quince fruit polysaccharides	1288:1323	Chinese quince fruit polysaccharides in food and pharmaceutical industries	1288:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	1	26	theme	cell	181:184	arg1	polysaccharides					191:205	cell wall polysaccharides	181:205	cell wall polysaccharides	181:205	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg1	fruits					492:497	Chinese quince fruits	477:497	Chinese quince fruits	477:497	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	hemicellulose					435:447	4 mol/L KOH soluble hemicellulose	415:447	4 mol/L KOH soluble hemicellulose (KSH-2)	415:455	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	pectin					285:290	water-soluble pectin	271:290	water-soluble pectin (WSP)	271:296	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	pectin					316:321	chelator-soluble pectin	299:321	chelator-soluble pectin (CSP)	299:327	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	pectin					355:360	sodium carbonate-soluble pectin	330:360	sodium carbonate-soluble pectin (NSP)	330:366	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	fractions					253:261	two hemicellulose fractions	235:261	two hemicellulose fractions	235:261	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	27	attach	isolated	463:470	arg2	hemicellulose					389:401	1 mol/L KOH soluble hemicellulose	369:401	1 mol/L KOH soluble hemicellulose (KSH-1)	369:409	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	8	28	theme	main	1027:1030	arg1	chains					1032:1037	RG-I and galacturonic acid main chains	1000:1037	RG-I and galacturonic acid main chains	1000:1037	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	9	29	theme	highest	1154:1160	arg1	activity					1186:1193	the highest DPPH radical scavenging activity	1150:1193	the highest DPPH radical scavenging activity	1150:1193	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	1	30	theme	wall	186:189	arg1	polysaccharides					191:205	cell wall polysaccharides	181:205	cell wall polysaccharides	181:205	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	8	31	theme	acid	1022:1025	arg1	chains					1032:1037	RG-I and galacturonic acid main chains	1000:1037	RG-I and galacturonic acid main chains	1000:1037	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	7	32	theme	molecular	889:897	arg1	weight					899:904	molecular weight	889:904	the highest molecular weight	877:904	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	4	33	theme	esterification	701:714	arg1	%					726:726	31.7-42.4%	717:726	31.7-42.4%	717:726	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	4	33	theme	esterification	701:714	arg1	degree					691:696	a low degree	685:696	a low degree of esterification (31.7-42.4%)	685:727	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	1	34	theme	polysaccharides	191:205	arg1	composition					135:145	the composition	131:145	the composition	131:145	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	34	theme	polysaccharides	191:205	arg1	characteristics					162:176	structural characteristics	151:176	structural characteristics	151:176	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	9	35	theme	DPPH	1162:1165	arg1	activity					1186:1193	the highest DPPH radical scavenging activity	1150:1193	the highest DPPH radical scavenging activity	1150:1193	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	9	36	theme	radical	1167:1173	arg1	activity					1186:1193	the highest DPPH radical scavenging activity	1150:1193	the highest DPPH radical scavenging activity	1150:1193	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	10	37	from	polysaccharides	1309:1323	arg1	industries					1352:1361	food and pharmaceutical industries	1328:1361	food and pharmaceutical industries	1328:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	0	38	theme	rheological	11:21	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	38	theme	rheological	11:21	arg1	properties					48:57	rheological, thermal and antioxidant properties	11:57	rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits	11:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	6	39	theme	polysaccharide	807:820	arg1	lengths					828:834	The polysaccharide chain lengths	803:834	The polysaccharide chain lengths	803:834	The polysaccharide chain lengths ranged from 19.4 nm to 121.4 nm.					
31726165	3	40	theme	polysaccharide	622:635	arg1	fraction					637:644	the predominant cell wall polysaccharide fraction	596:644	the predominant cell wall polysaccharide fraction in the fruit	596:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	8	41	theme	1,5-α-L-Araf	1096:1107	arg1	units					1109:1113	1,5-α-L-Araf units	1096:1113	1,5-α-L-Araf units	1096:1113	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	8	42	theme	galacturonic	1009:1020	arg1	chains					1032:1037	RG-I and galacturonic acid main chains	1000:1037	RG-I and galacturonic acid main chains	1000:1037	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	1	43	theme	1 mol/L	369:375	arg1	KSH-1					404:408	KSH-1	404:408	KSH-1	404:408	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	43	theme	1 mol/L	369:375	arg1	hemicellulose					389:401	1 mol/L KOH soluble hemicellulose	369:401	1 mol/L KOH soluble hemicellulose (KSH-1)	369:409	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	43	theme	1 mol/L	369:375	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	3	44	dep	showed	581:586	arg1	fraction					637:644	the predominant cell wall polysaccharide fraction	596:644	the predominant cell wall polysaccharide fraction in the fruit	596:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	1	45	theme	pectic	214:219	arg1	pectin					316:321	chelator-soluble pectin	299:321	chelator-soluble pectin (CSP)	299:327	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	45	theme	pectic	214:219	arg1	pectin					355:360	sodium carbonate-soluble pectin	330:360	sodium carbonate-soluble pectin (NSP)	330:366	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	45	theme	pectic	214:219	arg1	hemicellulose					389:401	1 mol/L KOH soluble hemicellulose	369:401	1 mol/L KOH soluble hemicellulose (KSH-1)	369:409	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	45	theme	pectic	214:219	arg1	hemicellulose					435:447	4 mol/L KOH soluble hemicellulose	415:447	4 mol/L KOH soluble hemicellulose (KSH-2)	415:455	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	45	theme	pectic	214:219	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	45	theme	pectic	214:219	arg1	pectin					285:290	water-soluble pectin	271:290	water-soluble pectin (WSP)	271:296	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	0	46	theme	thermal	24:30	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	46	theme	thermal	24:30	arg1	properties					48:57	rheological, thermal and antioxidant properties	11:57	rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits	11:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	1	47	theme	chelator-soluble	299:314	arg1	pectin					316:321	chelator-soluble pectin	299:321	chelator-soluble pectin (CSP)	299:327	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	47	theme	chelator-soluble	299:314	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	47	theme	chelator-soluble	299:314	arg1	CSP					324:326	CSP	324:326	CSP	324:326	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	48	theme	KOH	377:379	arg1	KSH-1					404:408	KSH-1	404:408	KSH-1	404:408	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	48	theme	KOH	377:379	arg1	hemicellulose					389:401	1 mol/L KOH soluble hemicellulose	369:401	1 mol/L KOH soluble hemicellulose (KSH-1)	369:409	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	48	theme	KOH	377:379	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	10	49	theme	quince	1296:1301	arg1	polysaccharides					1309:1323	Chinese quince fruit polysaccharides	1288:1323	Chinese quince fruit polysaccharides in food and pharmaceutical industries	1288:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	3	50	from	fraction	637:644	arg1	fruit					653:657	the fruit	649:657	the fruit	649:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	5	51	theme	highest	751:757	arg1	stability					767:775	the highest thermal stability	747:775	the highest thermal stability	747:775	WSP fraction had the highest thermal stability among the five fractions.					
31726165	5	52	theme	thermal	759:765	arg1	stability					767:775	the highest thermal stability	747:775	the highest thermal stability	747:775	WSP fraction had the highest thermal stability among the five fractions.					
31726165	1	53	theme	soluble	381:387	arg1	KSH-1					404:408	KSH-1	404:408	KSH-1	404:408	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	53	theme	soluble	381:387	arg1	hemicellulose					389:401	1 mol/L KOH soluble hemicellulose	369:401	1 mol/L KOH soluble hemicellulose (KSH-1)	369:409	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	1	53	theme	soluble	381:387	arg1	fractions					221:229	three pectic fractions	208:229	three pectic fractions	208:229	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	10	54	theme	Chinese	1288:1294	arg1	polysaccharides					1309:1323	Chinese quince fruit polysaccharides	1288:1323	Chinese quince fruit polysaccharides in food and pharmaceutical industries	1288:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	4	55	theme	low	687:689	arg1	%					726:726	31.7-42.4%	717:726	31.7-42.4%	717:726	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	4	55	theme	low	687:689	arg1	degree					691:696	a low degree	685:696	a low degree of esterification (31.7-42.4%)	685:727	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	0	56	theme	antioxidant	36:46	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	56	theme	antioxidant	36:46	arg1	properties					48:57	rheological, thermal and antioxidant properties	11:57	rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits	11:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	2	57	theme	compositional	544:556	arg1	variation					558:566	structural and compositional variation	529:566	structural and compositional variation	529:566	The five fractions exhibited structural and compositional variation.					
31726165	0	58	from	fruits	108:113	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	58	from	fruits	108:113	arg1	polysaccharides					72:86	cell wall polysaccharides	62:86	cell wall polysaccharides from Chinese quince fruits	62:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	0	58	from	fruits	108:113	arg1	properties					48:57	rheological, thermal and antioxidant properties	11:57	rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits	11:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	3	59	theme	cell	612:615	arg1	fraction					637:644	the predominant cell wall polysaccharide fraction	596:644	the predominant cell wall polysaccharide fraction in the fruit	596:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	7	60	dep	it	914:915	arg1	viscosity					943:951	the highest solution viscosity	922:951	it also the highest solution viscosity	914:951	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	8	61	theme	NMR	954:956	arg1	spectra					958:964	NMR spectra	954:964	NMR spectra	954:964	NMR spectra revealed that NSP was composed of RG-I and galacturonic acid main chains, KSH-1 was composed of 1,4-β-D-Xylp backbone attached to 1,5-α-L-Araf units.					
31726165	7	62	theme	highest	926:932	arg1	viscosity					943:951	the highest solution viscosity	922:951	it also the highest solution viscosity	914:951	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	10	63	theme	pharmaceutical	1337:1350	arg1	industries					1352:1361	food and pharmaceutical industries	1328:1361	food and pharmaceutical industries	1328:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	7	64	theme	highest	881:887	arg1	weight					899:904	molecular weight	889:904	the highest molecular weight	877:904	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
31726165	9	65	contain	has	1146:1148	arg1	CSP					1142:1144	CSP	1142:1144	CSP	1142:1144	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	9	65	contain	has	1146:1148	arg2	activity					1186:1193	the highest DPPH radical scavenging activity	1150:1193	the highest DPPH radical scavenging activity	1150:1193	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	2	66	theme	structural	529:538	arg1	variation					558:566	structural and compositional variation	529:566	structural and compositional variation	529:566	The five fractions exhibited structural and compositional variation.					
31726165	9	67	theme	highest	1215:1221	arg1	power					1232:1236	the highest reducing power	1211:1236	the highest reducing power	1211:1236	Among the five fractions, CSP has the highest DPPH radical scavenging activity while KSH-1 has the highest reducing power.					
31726165	3	68	theme	predominant	600:610	arg1	fraction					637:644	the predominant cell wall polysaccharide fraction	596:644	the predominant cell wall polysaccharide fraction in the fruit	596:657	The results showed NSP was the predominant cell wall polysaccharide fraction in the fruit.					
31726165	1	69	theme	Chinese	477:483	arg1	fruits					492:497	Chinese quince fruits	477:497	Chinese quince fruits	477:497	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	10	70	from	industries	1352:1361	arg1	applications					1272:1283	the applications	1268:1283	the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries	1268:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	0	71	theme	cell	62:65	arg1	polysaccharides					72:86	cell wall polysaccharides	62:86	cell wall polysaccharides from Chinese quince fruits	62:113	Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits.					
31726165	5	72	contain	had	743:745	arg2	stability					767:775	the highest thermal stability	747:775	the highest thermal stability	747:775	WSP fraction had the highest thermal stability among the five fractions.					
31726165	5	72	contain	had	743:745	arg1	fraction					734:741	WSP fraction	730:741	WSP fraction	730:741	WSP fraction had the highest thermal stability among the five fractions.					
31726165	1	73	theme	quince	485:490	arg1	fruits					492:497	Chinese quince fruits	477:497	Chinese quince fruits	477:497	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	4	74	theme	pectic	664:669	arg1	fractions					671:679	All pectic fractions	660:679	All pectic fractions	660:679	All pectic fractions had a low degree of esterification (31.7-42.4%).					
31726165	10	75	from	applications	1272:1283	arg1	industries					1352:1361	food and pharmaceutical industries	1328:1361	food and pharmaceutical industries	1328:1361	This study can contribute to the applications of Chinese quince fruit polysaccharides in food and pharmaceutical industries.					
31726165	1	76	theme	hemicellulose	239:251	arg1	fractions					253:261	two hemicellulose fractions	235:261	two hemicellulose fractions	235:261	To investigate the composition and structural characteristics of cell wall polysaccharides, three pectic fractions and two hemicellulose fractions, namely water-soluble pectin (WSP), chelator-soluble pectin (CSP), sodium carbonate-soluble pectin (NSP), 1 mol/L KOH soluble hemicellulose (KSH-1) and 4 mol/L KOH soluble hemicellulose (KSH-2), were isolated from Chinese quince fruits.					
31726165	7	77	theme	solution	934:941	arg1	viscosity					943:951	the highest solution viscosity	922:951	it also the highest solution viscosity	914:951	CSP had the highest molecular weight, giving it also the highest solution viscosity.					
30957617	0	0	theme	oligozoospermic	95:109	arg1	men					111:113	normozoospermic and oligozoospermic men	75:113	normozoospermic and oligozoospermic men	75:113	Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men.					
30957617	5	1	theme	presentation	1165:1176	arg1	terms					1156:1160	terms	1156:1160	terms of presentation of sialylated and mannosylated moieties	1156:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	5	2	theme	moieties	1209:1216	arg1	presentation					1165:1176	presentation	1165:1176	presentation of sialylated and mannosylated moieties	1165:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	6	3	from	influence	1302:1310	arg1	surface					1381:1387	the prostasomal surface	1365:1387	the prostasomal surface	1365:1387	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	6	3	from	influence	1302:1310	arg1	accessibility					1319:1331	the accessibility	1315:1331	the accessibility of galectin-3 to its ligands	1315:1360	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	6	4	theme	galectin-3	1336:1345	arg1	accessibility					1319:1331	the accessibility	1315:1331	the accessibility of galectin-3 to its ligands	1315:1360	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	3	5	theme	normo-	607:612	arg1	men					634:636	normo- and oligozoospermic men	607:636	normo- and oligozoospermic men	607:636	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	1	6	theme	extracellular	329:341	arg1	environment					343:353	the extracellular environment	325:353	the extracellular environment	325:353	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	2	7	contain	have	423:426	arg2	signals					480:486	recognition signals	468:486	recognition signals	468:486	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	7	contain	have	423:426	arg2	biomarkers					453:462	biomarkers	453:462	biomarkers	453:462	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	7	contain	have	423:426	arg2	importance					439:448	biomedical importance	428:448	biomedical importance	428:448	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	7	contain	have	423:426	arg1	glycans					365:371	glycans	365:371	glycans	365:371	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	8	8	theme	distinct	1638:1645	arg1	populations					1647:1657	distinct populations	1638:1657	distinct populations of vesicles	1638:1669	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	5	9	from	frame	998:1002	arg1	composition					1037:1047	protein composition	1029:1047	protein composition	1029:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	1	10	theme	parent	268:273	arg1	cells					275:279	their parent cells	262:279	their parent cells	262:279	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	5	11	from	groups	1131:1136	arg1	terms					1156:1160	terms	1156:1160	terms of presentation of sialylated and mannosylated moieties	1156:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	7	12	theme	same	1525:1528	arg1	properties					1539:1548	the same physical properties	1521:1548	the same physical properties	1521:1548	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	3	13	from	plasma	592:597	arg1	EVs					575:577	EVs	575:577	EVs from seminal plasma (SP) of normo- and oligozoospermic men	575:636	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	4	14	attach	isolated	780:787	arg1	SP					801:802	pooled SP	794:802	pooled SP	794:802	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	4	14	attach	isolated	780:787	arg2	Prostasomes					763:773	Prostasomes	763:773	Prostasomes	763:773	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	5	15	theme	prostasomes	1141:1151	arg1	prostasomes					1141:1151	prostasomes	1141:1151	prostasomes	1141:1151	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	5	15	theme	prostasomes	1141:1151	arg1	groups					1131:1136	the examined groups	1118:1136	the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties	1118:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	7	16	theme	surface	1445:1451	arg1	molecules					1453:1461	surface molecules	1445:1461	surface molecules	1445:1461	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	5	17	from	composition	1037:1047	arg1	frame					998:1002	the frame	994:1002	the frame of overall similarity in protein composition	994:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	4	18	dep	isolated	780:787	arg1	followed					855:862	followed	855:862	followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography	855:975	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	3	19	theme	possible	672:679	arg1	changes					681:687	possible changes	672:687	possible changes in their surface glycans	672:712	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	4	20	theme	differential	807:818	arg1	centrifugation					820:833	differential centrifugation	807:833	differential centrifugation	807:833	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	0	21	theme	Surface	0:6	arg1	glycans					8:14	Surface glycans	0:14	Surface glycans	0:14	Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men.					
30957617	5	22	from	similarity	1015:1024	arg1	composition					1037:1047	protein composition	1029:1047	protein composition	1029:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	1	23	theme	plasma	176:181	arg1	membrane					183:190	the plasma membrane	172:190	the plasma membrane	172:190	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	24	attach	released	158:165	arg1	membrane					183:190	the plasma membrane	172:190	the plasma membrane	172:190	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	24	attach	released	158:165	arg1	compartments					209:220	intracellular compartments	195:220	intracellular compartments	195:220	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	24	attach	released	158:165	arg2	EVs					152:154	EVs	152:154	EVs	152:154	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	24	attach	released	158:165	arg2	vesicles					142:149	Extracellular vesicles	128:149	Extracellular vesicles (EVs)	128:155	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	4	25	theme	gel	839:841	arg1	filtration					843:852	gel filtration	839:852	gel filtration	839:852	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	7	26	theme	physical	1530:1537	arg1	properties					1539:1548	the same physical properties	1521:1548	the same physical properties	1521:1548	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	8	27	with	interaction	1623:1633	arg1	partners					1690:1697	their binding partners	1676:1697	their binding partners	1676:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	2	28	theme	biomedical	428:437	arg1	biomarkers					453:462	biomarkers	453:462	biomarkers	453:462	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	28	theme	biomedical	428:437	arg1	signals					480:486	recognition signals	468:486	recognition signals	468:486	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	28	theme	biomedical	428:437	arg1	importance					439:448	biomedical importance	428:448	biomedical importance	428:448	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	5	29	theme	sialylated	1181:1190	arg1	presentation					1165:1176	presentation	1165:1176	presentation of sialylated and mannosylated moieties	1165:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	8	30	theme	binding	1682:1688	arg1	partners					1690:1697	their binding partners	1676:1697	their binding partners	1676:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	3	31	theme	altered	720:726	arg1	conditions					742:751	altered physiological conditions	720:751	altered physiological conditions	720:751	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	7	32	from	differences	1410:1420	arg1	presentation					1429:1440	the presentation	1425:1440	the presentation of surface molecules	1425:1461	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	7	33	theme	molecules	1453:1461	arg1	presentation					1429:1440	the presentation	1425:1440	the presentation of surface molecules	1425:1461	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	1	34	theme	intracellular	195:207	arg1	compartments					209:220	intracellular compartments	195:220	intracellular compartments	195:220	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	3	35	theme	surface	698:704	arg1	glycans					706:712	their surface glycans	692:712	their surface glycans	692:712	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	3	36	theme	seminal	549:555	arg1	prostasomes					557:567	seminal prostasomes	549:567	seminal prostasomes	549:567	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	2	37	theme	recognition	468:478	arg1	signals					480:486	recognition signals	468:486	recognition signals	468:486	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	2	37	theme	recognition	468:478	arg1	importance					439:448	biomedical importance	428:448	biomedical importance	428:448	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	8	38	theme	interaction	1623:1633	arg1	mechanisms					1609:1618	some unexpected regulatory mechanisms	1582:1618	some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners	1582:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	3	39	theme	oligozoospermic	618:632	arg1	men					634:636	normo- and oligozoospermic men	607:636	normo- and oligozoospermic men	607:636	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	4	40	theme	pooled	794:799	arg1	SP					801:802	pooled SP	794:802	pooled SP	794:802	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	8	41	theme	vesicles	1662:1669	arg1	populations					1647:1657	distinct populations	1638:1657	distinct populations of vesicles	1638:1669	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	2	42	theme	EV	400:401	arg1	composition					403:413	EV composition	400:413	EV composition	400:413	Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated.					
30957617	8	43	theme	populations	1647:1657	arg1	interaction					1623:1633	interaction	1623:1633	interaction of distinct populations of vesicles with their binding partners	1623:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	4	44	theme	ion-exchange	949:960	arg1	chromatography					962:975	ion-exchange chromatography	949:975	ion-exchange chromatography	949:975	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	5	45	theme	examined	1122:1129	arg1	prostasomes					1141:1151	prostasomes	1141:1151	prostasomes	1141:1151	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	5	45	theme	examined	1122:1129	arg1	groups					1131:1136	the examined groups	1118:1136	the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties	1118:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	3	46	theme	seminal	584:590	arg1	SP					600:601	SP	600:601	SP	600:601	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	3	46	theme	seminal	584:590	arg1	plasma					592:597	seminal plasma	584:597	seminal plasma (SP) of normo- and oligozoospermic men	584:636	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	5	47	theme	mannosylated	1196:1207	arg1	moieties					1209:1216	mannosylated moieties	1196:1216	mannosylated moieties	1196:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	1	48	mod	modified	313:320	arg1	they					286:289	they	286:289	they	286:289	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	48	mod	modified	313:320	arg3	environment					343:353	the extracellular environment	325:353	the extracellular environment	325:353	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	8	49	theme	regulatory	1598:1607	arg1	mechanisms					1609:1618	some unexpected regulatory mechanisms	1582:1618	some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners	1582:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	1	50	theme	Extracellular	128:140	arg1	EVs					152:154	EVs	152:154	EVs	152:154	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	50	theme	Extracellular	128:140	arg1	vesicles					142:149	Extracellular vesicles	128:149	Extracellular vesicles (EVs)	128:155	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	0	51	theme	seminal	50:56	arg1	prostasomes					58:68	seminal prostasomes	50:68	seminal prostasomes from normozoospermic and oligozoospermic men	50:113	Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men.					
30957617	3	52	theme	physiological	728:740	arg1	conditions					742:751	altered physiological conditions	720:751	altered physiological conditions	720:751	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	4	53	theme	glycobiochemical	867:882	arg1	characterization					884:899	glycobiochemical characterization	867:899	glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography	867:975	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	1	54	contain	have	223:226	arg1	EVs					152:154	EVs	152:154	EVs	152:154	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	54	contain	have	223:226	arg1	vesicles					142:149	Extracellular vesicles	128:149	Extracellular vesicles (EVs)	128:155	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	1	54	contain	have	223:226	arg2	composition					239:249	a specific composition	228:249	a specific composition related to their parent cells	228:279	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	7	55	theme	Subtle	1403:1408	arg1	differences					1410:1420	Subtle differences	1403:1420	Subtle differences in the presentation of surface molecules	1403:1461	Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties.					
30957617	4	56	theme	lectin/immune-transmission	907:932	arg1	microscopy					934:943	lectin/immune-transmission microscopy	907:943	lectin/immune-transmission microscopy	907:943	Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography.					
30957617	3	57	from	changes	681:687	arg1	glycans					706:712	their surface glycans	692:712	their surface glycans	692:712	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	5	58	theme	similarity	1015:1024	arg1	frame					998:1002	the frame	994:1002	the frame of overall similarity in protein composition	994:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	5	59	theme	surface	1050:1056	arg1	glycans					1058:1064	surface glycans	1050:1064	surface glycans	1050:1064	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	6	60	theme	prostasomal	1369:1379	arg1	surface					1381:1387	the prostasomal surface	1365:1387	the prostasomal surface	1365:1387	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	5	61	theme	overall	1007:1013	arg1	similarity					1015:1024	overall similarity	1007:1024	overall similarity in protein composition	1007:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	3	62	theme	men	634:636	arg1	SP					600:601	SP	600:601	SP	600:601	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	3	62	theme	men	634:636	arg1	plasma					592:597	seminal plasma	584:597	seminal plasma (SP) of normo- and oligozoospermic men	584:636	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	6	63	theme	anti-oxidative	1254:1267	arg1	capacity					1269:1276	their anti-oxidative capacity	1248:1276	their anti-oxidative capacity	1248:1276	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	5	64	gly	mannosylated	1196:1207	arg1	moieties					1209:1216	mannosylated moieties	1196:1216	mannosylated moieties	1196:1216	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	1	65	theme	specific	230:237	arg1	composition					239:249	a specific composition	228:249	a specific composition related to their parent cells	228:279	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
30957617	0	66	theme	normozoospermic	75:89	arg1	men					111:113	normozoospermic and oligozoospermic men	75:113	normozoospermic and oligozoospermic men	75:113	Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men.					
30957617	0	67	from	men	111:113	arg1	prostasomes					58:68	seminal prostasomes	50:68	seminal prostasomes from normozoospermic and oligozoospermic men	50:113	Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men.					
30957617	5	68	theme	protein	1029:1035	arg1	composition					1037:1047	protein composition	1029:1047	protein composition	1029:1047	Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties.					
30957617	8	69	theme	unexpected	1587:1596	arg1	mechanisms					1609:1618	some unexpected regulatory mechanisms	1582:1618	some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners	1582:1697	In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.					
30957617	3	70	dep	EVs	575:577	arg1	i.e.					570:573	i.e.	570:573	i.e.	570:573	In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions.					
30957617	6	71	theme	possible	1293:1300	arg1	influence					1302:1310	a possible influence	1291:1310	a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface	1291:1387	These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface.					
30957617	1	72	theme	related	251:257	arg1	composition					239:249	a specific composition	228:249	a specific composition related to their parent cells	228:279	Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment.					
31545132	6	0	theme	scanning	1009:1016	arg1	calorimetry					1018:1028	differential scanning calorimetry	996:1028	differential scanning calorimetry	996:1028	The specimens were also inspected by differential scanning calorimetry and photomicrographs.					
31545132	7	1	theme	best	1086:1089	arg1	performance					1091:1101	the best performance	1082:1101	the best performance	1082:1101	Finally, the specimen showing the best performance from the thermomechanical viewpoint has been selected to extrude the filament for the fused deposition modelling process.					
31545132	6	2	theme	differential	996:1007	arg1	calorimetry					1018:1028	differential scanning calorimetry	996:1028	differential scanning calorimetry	996:1028	The specimens were also inspected by differential scanning calorimetry and photomicrographs.					
31545132	1	3	theme	experimental	67:78	arg1	study					80:84	this experimental study	62:84	this experimental study	62:84	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	3	4	theme	deposition	607:616	arg1	modelling					618:626	fused deposition modelling	601:626	fused deposition modelling	601:626	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	4	5	theme	Taguchi-based	666:678	arg1	design					680:685	Taguchi-based design	666:685	Taguchi-based design of experiments	666:700	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	5	theme	Taguchi-based	666:678	arg1	follows					765:771	follows	765:771	follows	765:771	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	7	6	theme	modelling	1206:1214	arg1	process					1216:1222	the fused deposition modelling process	1185:1222	the fused deposition modelling process	1185:1222	Finally, the specimen showing the best performance from the thermomechanical viewpoint has been selected to extrude the filament for the fused deposition modelling process.					
31545132	4	7	dep	follows	765:771	arg1	load					787:790	load applied	787:798	load applied	787:798	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	7	dep	follows	765:771	arg1	temperature					774:784	temperature	774:784	temperature	774:784	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	7	dep	follows	765:771	arg1	composition/proportions					814:836	different composition/proportions	804:836	different composition/proportions	804:836	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	2	8	theme	different	439:447	arg1	compositions/proportions					449:472	different compositions/proportions	439:472	different compositions/proportions	439:472	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	1	9	theme	possible	197:204	arg1	applications					206:217	possible applications	197:217	possible applications as orthopaedic scaffolds	197:242	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	7	10	theme	fused	1189:1193	arg1	process					1216:1222	the fused deposition modelling process	1185:1222	the fused deposition modelling process	1185:1222	Finally, the specimen showing the best performance from the thermomechanical viewpoint has been selected to extrude the filament for the fused deposition modelling process.					
31545132	0	11	theme	Thermomechanical	0:15	arg1	investigations					17:30	Thermomechanical investigations	0:30	Thermomechanical investigations of PEKK-HAp-CS composites	0:56	Thermomechanical investigations of PEKK-HAp-CS composites.					
31545132	7	12	theme	deposition	1195:1204	arg1	process					1216:1222	the fused deposition modelling process	1185:1222	the fused deposition modelling process	1185:1222	Finally, the specimen showing the best performance from the thermomechanical viewpoint has been selected to extrude the filament for the fused deposition modelling process.					
31545132	5	13	theme	tensile	942:948	arg1	testing					950:956	tensile testing	942:956	tensile testing	942:956	The specimens in the form of feedstock filament produced by the extrusion process were made to undergo tensile testing.					
31545132	4	14	theme	different	804:812	arg1	composition/proportions					814:836	different composition/proportions	804:836	different composition/proportions	804:836	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	15	from	variables	727:735	arg1	process					754:760	the extrusion process	740:760	the extrusion process	740:760	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	16	theme	extrusion	744:752	arg1	process					754:760	the extrusion process	740:760	the extrusion process	740:760	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	0	17	theme	PEKK-HAp-CS	35:45	arg1	composites					47:56	PEKK-HAp-CS composites	35:56	PEKK-HAp-CS composites	35:56	Thermomechanical investigations of PEKK-HAp-CS composites.					
31545132	1	18	theme	poly-ether-ketone-ketone	102:125	arg1	poly-ether-ketone-ketone					102:125	poly-ether-ketone-ketone	102:125	poly-ether-ketone-ketone	102:125	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	1	18	theme	poly-ether-ketone-ketone	102:125	arg1	composite					89:97	a composite	87:97	a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan	87:173	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	1	19	theme	orthopaedic	222:232	arg1	scaffolds					234:242	orthopaedic scaffolds	222:242	orthopaedic scaffolds	222:242	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	4	20	theme	thermomechanical	633:648	arg1	investigations					650:663	thermomechanical investigations	633:663	thermomechanical investigations	633:663	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	2	21	theme	poly-ether-ketone-ketone	339:362	arg1	matrix					364:369	the poly-ether-ketone-ketone matrix	335:369	the poly-ether-ketone-ketone matrix	335:369	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	5	22	theme	extrusion	903:911	arg1	process					913:919	the extrusion process	899:919	the extrusion process	899:919	The specimens in the form of feedstock filament produced by the extrusion process were made to undergo tensile testing.					
31545132	3	23	theme	Suitable	475:482	arg1	compositions					484:495	Suitable compositions	475:495	Suitable compositions revealed by the melt flow index test	475:532	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	4	24	theme	input	721:725	arg1	variables					727:735	input variables	721:735	input variables in the extrusion process	721:760	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	3	25	theme	flow	518:521	arg1	test					529:532	the melt flow index test	509:532	the melt flow index test	509:532	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	0	26	theme	composites	47:56	arg1	investigations					17:30	Thermomechanical investigations	0:30	Thermomechanical investigations of PEKK-HAp-CS composites	0:56	Thermomechanical investigations of PEKK-HAp-CS composites.					
31545132	5	27	theme	feedstock	868:876	arg1	filament					878:885	feedstock filament	868:885	feedstock filament produced by the extrusion process	868:919	The specimens in the form of feedstock filament produced by the extrusion process were made to undergo tensile testing.					
31545132	2	28	theme	weight	266:271	arg1	percentages					273:283	different weight percentages	256:283	different weight percentages of hydroxyapatite and chitosan	256:314	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	3	29	theme	index	523:527	arg1	test					529:532	the melt flow index test	509:532	the melt flow index test	509:532	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	5	30	theme	filament	878:885	arg1	form					860:863	the form	856:863	the form of feedstock filament produced by the extrusion process	856:919	The specimens in the form of feedstock filament produced by the extrusion process were made to undergo tensile testing.					
31545132	2	31	theme	different	256:264	arg1	percentages					273:283	different weight percentages	256:283	different weight percentages of hydroxyapatite and chitosan	256:314	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	2	32	theme	flow	391:394	arg1	index					396:400	melt flow index	386:400	melt flow index	386:400	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	2	33	theme	chitosan	307:314	arg1	percentages					273:283	different weight percentages	256:283	different weight percentages of hydroxyapatite and chitosan	256:314	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	7	34	theme	thermomechanical	1112:1127	arg1	viewpoint					1129:1137	the thermomechanical viewpoint	1108:1137	the thermomechanical viewpoint	1108:1137	Finally, the specimen showing the best performance from the thermomechanical viewpoint has been selected to extrude the filament for the fused deposition modelling process.					
31545132	1	35	theme	hydroxyapatite	147:160	arg1	reinforcement					130:142	reinforcement	130:142	reinforcement of hydroxyapatite and chitosan	130:173	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	4	36	theme	experiments	690:700	arg1	design					680:685	Taguchi-based design	666:685	Taguchi-based design of experiments	666:700	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	36	theme	experiments	690:700	arg1	follows					765:771	follows	765:771	follows	765:771	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	5	37	from	specimens	843:851	arg1	form					860:863	the form	856:863	the form of feedstock filament produced by the extrusion process	856:919	The specimens in the form of feedstock filament produced by the extrusion process were made to undergo tensile testing.					
31545132	3	38	theme	fused	601:605	arg1	modelling					618:626	fused deposition modelling	601:626	fused deposition modelling	601:626	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	4	39	theme	applied	792:798	arg1	load					787:790	load applied	787:798	load applied	787:798	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	2	40	theme	melt	386:389	arg1	index					396:400	melt flow index	386:400	melt flow index	386:400	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	2	41	theme	compositions/proportions	449:472	arg1	flowability					424:434	the flowability	420:434	the flowability of different compositions/proportions	420:472	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	2	42	theme	hydroxyapatite	288:301	arg1	percentages					273:283	different weight percentages	256:283	different weight percentages of hydroxyapatite and chitosan	256:314	Initially, different weight percentages of hydroxyapatite and chitosan were reinforced in the poly-ether-ketone-ketone matrix and tested for melt flow index in order to check the flowability of different compositions/proportions.					
31545132	1	43	theme	chitosan	166:173	arg1	reinforcement					130:142	reinforcement	130:142	reinforcement of hydroxyapatite and chitosan	130:173	In this experimental study, a composite of poly-ether-ketone-ketone by reinforcement of hydroxyapatite and chitosan has been prepared for possible applications as orthopaedic scaffolds.					
31545132	3	44	theme	melt	513:516	arg1	test					529:532	the melt flow index test	509:532	the melt flow index test	509:532	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
31545132	4	45	used	used	711:714	arg2	design					680:685	Taguchi-based design	666:685	Taguchi-based design of experiments	666:700	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	4	45	used	used	711:714	arg2	follows					765:771	follows	765:771	follows	765:771	For thermomechanical investigations, Taguchi-based design of experiments has been used with input variables in the extrusion process as follows: temperature, load applied and different composition/proportions.					
31545132	3	46	theme	filament	579:586	arg1	extrusion					566:574	the extrusion	562:574	the extrusion of filament required for fused deposition modelling	562:626	Suitable compositions revealed by the melt flow index test were then taken forward for the extrusion of filament required for fused deposition modelling.					
30145003	4	0	theme	neutron	709:715	arg1	scattering					717:726	neutron scattering	709:726	neutron scattering	709:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	1	from	polymer	698:704	arg1	scattering					717:726	neutron scattering	709:726	neutron scattering	709:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	3	2	theme	molecular	561:569	arg1	47-50 kDa					579:587	47-50 kDa	579:587	47-50 kDa	579:587	Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47-50 kDa).					
30145003	3	2	theme	molecular	561:569	arg1	weight					571:576	molecular weight	561:576	molecular weight (47-50 kDa)	561:588	Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47-50 kDa).					
30145003	4	3	theme	Structural	591:600	arg1	similarities					602:613	Structural similarities	591:613	Structural similarities	591:613	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	4	theme	immunomodulatory	768:783	arg1	activity					785:792	their immunomodulatory activity	762:792	their immunomodulatory activity	762:792	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	2	5	attach	isolated	467:474	arg1	switchgrass					491:501	protiated switchgrass	481:501	protiated switchgrass	481:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	5	attach	isolated	467:474	arg2	hemicellulose					453:465	hemicellulose	453:465	hemicellulose isolated from protiated switchgrass	453:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	1	6	theme	structural	82:91	arg1	characterization					93:108	the structural characterization	78:108	the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium	78:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	7	from	switchgrass	162:172	arg1	medium					181:186	H2O medium	177:186	H2O medium (protiated)	177:198	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	7	from	switchgrass	162:172	arg1	%					205:205	50%	203:205	50%	203:205	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	0	8	theme	Hemicellulose	0:12	arg1	characterization					14:29	Hemicellulose characterization	0:29	Hemicellulose characterization of deuterated switchgrass.	0:56	Hemicellulose characterization of deuterated switchgrass.					
30145003	2	9	theme	4-O-methyl	299:308	arg1	GAX					333:335	GAX	333:335	GAX	333:335	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	9	theme	4-O-methyl	299:308	arg1	glucuronoarabinoxylan					310:330	4-O-methyl glucuronoarabinoxylan	299:330	4-O-methyl glucuronoarabinoxylan (GAX)	299:336	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	9	theme	4-O-methyl	299:308	arg1	hemicellulose					349:361	the major hemicellulose	339:361	the major hemicellulose in switchgrass isolated from deuterated switchgrass	339:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	4	10	theme	model	679:683	arg1	polymer					698:704	a model carbohydrate polymer	677:704	a model carbohydrate polymer in neutron scattering	677:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	10	theme	model	679:683	arg1	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	2	11	theme	similar	442:448	arg1	properties					431:440	structural properties	420:440	structural properties similar to hemicellulose isolated from protiated switchgrass	420:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	12	from	hemicellulose	349:361	arg1	switchgrass					366:376	switchgrass	366:376	switchgrass isolated from deuterated switchgrass	366:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	1	13	theme	hemicellulose	113:125	arg1	characterization					93:108	the structural characterization	78:108	the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium	78:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	0	14	theme	deuterated	34:43	arg1	switchgrass					45:55	deuterated switchgrass	34:55	deuterated switchgrass	34:55	Hemicellulose characterization of deuterated switchgrass.					
30145003	4	15	from	studies	747:753	arg1	scattering					717:726	neutron scattering	709:726	neutron scattering	709:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	1	16	theme	D2O	207:209	arg1	medium					211:216	D2O medium	207:216	D2O medium	207:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	2	17	theme	structural	420:429	arg1	properties					431:440	structural properties	420:440	structural properties similar to hemicellulose isolated from protiated switchgrass	420:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	4	18	theme	gastroprotective	798:813	arg1	effects					815:821	gastroprotective effects	798:821	gastroprotective effects	798:821	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	1	19	dep	medium	181:186	arg1	medium					211:216	D2O medium	207:216	D2O medium	207:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	19	dep	medium	181:186	arg1	protiated					189:197	protiated	189:197	protiated	189:197	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	4	20	theme	switchgrass	636:646	arg1	studies					747:753	pharmaceutical studies	732:753	pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects	732:821	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	20	theme	switchgrass	636:646	arg1	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	20	theme	switchgrass	636:646	arg1	polymer					698:704	a model carbohydrate polymer	677:704	a model carbohydrate polymer in neutron scattering	677:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	2	21	theme	protiated	481:489	arg1	switchgrass					491:501	protiated switchgrass	481:501	protiated switchgrass	481:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	1	22	theme	1H/13C	282:287	arg1	NMR					294:296	1H/13C HSQC NMR	282:296	1H/13C HSQC NMR	282:296	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	0	23	theme	switchgrass	45:55	arg1	characterization					14:29	Hemicellulose characterization	0:29	Hemicellulose characterization of deuterated switchgrass.	0:56	Hemicellulose characterization of deuterated switchgrass.					
30145003	1	24	theme	HSQC	289:292	arg1	NMR					294:296	1H/13C HSQC NMR	282:296	1H/13C HSQC NMR	282:296	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	4	25	theme	deuterated	625:634	arg1	studies					747:753	pharmaceutical studies	732:753	pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects	732:821	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	25	theme	deuterated	625:634	arg1	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	25	theme	deuterated	625:634	arg1	polymer					698:704	a model carbohydrate polymer	677:704	a model carbohydrate polymer in neutron scattering	677:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	2	26	theme	deuterated	392:401	arg1	switchgrass					403:413	deuterated switchgrass	392:413	deuterated switchgrass	392:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	27	attach	isolated	378:385	arg1	switchgrass					403:413	deuterated switchgrass	392:413	deuterated switchgrass	392:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	27	attach	isolated	378:385	arg2	switchgrass					366:376	switchgrass	366:376	switchgrass isolated from deuterated switchgrass	366:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	4	28	used	used	669:672	arg2	studies					747:753	pharmaceutical studies	732:753	pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects	732:821	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	28	used	used	669:672	arg2	polymer					698:704	a model carbohydrate polymer	677:704	a model carbohydrate polymer in neutron scattering	677:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	28	used	used	669:672	arg2	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	1	29	theme	grown	156:160	arg1	switchgrass					162:172	hydroponically grown switchgrass	141:172	hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium	141:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	30	attach	isolated	127:134	arg1	switchgrass					162:172	hydroponically grown switchgrass	141:172	hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium	141:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	30	attach	isolated	127:134	arg2	hemicellulose					113:125	hemicellulose	113:125	hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium	113:216	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	4	31	theme	carbohydrate	685:696	arg1	polymer					698:704	a model carbohydrate polymer	677:704	a model carbohydrate polymer in neutron scattering	677:726	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	31	theme	carbohydrate	685:696	arg1	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	1	32	dep	describes	68:76	arg1	deuterated					219:228	deuterated	219:228	deuterated	219:228	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	3	33	theme	xylose	537:542	arg1	0.25					551:554	0.25	551:554	0.25	551:554	Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47-50 kDa).					
30145003	3	33	theme	xylose	537:542	arg1	ratio					544:548	xylose ratio	537:548	xylose ratio (0.25)	537:555	Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47-50 kDa).					
30145003	4	34	theme	pharmaceutical	732:745	arg1	studies					747:753	pharmaceutical studies	732:753	pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects	732:821	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	4	34	theme	pharmaceutical	732:745	arg1	hemicellulose					648:660	deuterated switchgrass hemicellulose	625:660	deuterated switchgrass hemicellulose	625:660	Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.					
30145003	2	35	contain	had	416:418	arg1	GAX					333:335	GAX	333:335	GAX	333:335	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	35	contain	had	416:418	arg1	glucuronoarabinoxylan					310:330	4-O-methyl glucuronoarabinoxylan	299:330	4-O-methyl glucuronoarabinoxylan (GAX)	299:336	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	35	contain	had	416:418	arg2	properties					431:440	structural properties	420:440	structural properties similar to hemicellulose isolated from protiated switchgrass	420:501	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	35	contain	had	416:418	arg1	hemicellulose					349:361	the major hemicellulose	339:361	the major hemicellulose in switchgrass isolated from deuterated switchgrass	339:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	1	36	theme	H2O	177:179	arg1	medium					181:186	H2O medium	177:186	H2O medium (protiated)	177:198	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	1	37	theme	compositional	239:251	arg1	analysis					253:260	compositional analysis	239:260	compositional analysis	239:260	This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR.					
30145003	2	38	theme	major	343:347	arg1	glucuronoarabinoxylan					310:330	4-O-methyl glucuronoarabinoxylan	299:330	4-O-methyl glucuronoarabinoxylan (GAX)	299:336	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
30145003	2	38	theme	major	343:347	arg1	hemicellulose					349:361	the major hemicellulose	339:361	the major hemicellulose in switchgrass isolated from deuterated switchgrass	339:413	4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass.					
32110292	8	0	theme	high	1578:1581	arg1	similarity					1583:1592	high similarity	1578:1592	high similarity in sequence	1578:1604	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	3	1	theme	single	611:616	arg1	site					634:637	only a single N-glycosylation site	604:637	only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence	604:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	2	2	theme	interest	279:286	arg1	increase					267:274	a steady increase	258:274	a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases	258:375	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	10	3	theme	large-scale	1958:1968	arg1	profiling					1984:1992	large-scale site-specific profiling	1958:1992	large-scale site-specific profiling of glycoproteomes	1958:2010	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	10	4	from	serum	1876:1880	arg1	glycopeptides					1851:1863	intact glycopeptides	1844:1863	intact glycopeptides from human serum	1844:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	7	5	theme	N-glycosylation	1393:1407	arg1	profile					1409:1415	the N-glycosylation profile	1389:1415	the N-glycosylation profile of IgG	1389:1422	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	6	6	theme	chemical	1081:1088	arg1	labeling					1090:1097	chemical labeling	1081:1097	a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1079:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	3	7	theme	N-glycosylation	618:632	arg1	site					634:637	only a single N-glycosylation site	604:637	only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence	604:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	8	8	from	similarity	1583:1592	arg1	sequence					1597:1604	sequence	1597:1604	sequence	1597:1604	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	3	9	from	site	634:637	arg1	region					649:654	the Fc region	642:654	the Fc region with a highly similar amino acid sequence	642:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	6	10	theme	mass	1168:1171	arg1	analysis					1186:1193	mass spectrometry analysis	1168:1193	mass spectrometry analysis	1168:1193	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	3	11	theme	Fc	646:647	arg1	region					649:654	the Fc region	642:654	the Fc region with a highly similar amino acid sequence	642:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	5	12	theme	sequence	977:984	arg1	coverage					986:993	partial sequence coverage	969:993	partial sequence coverage	969:993	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	3	13	theme	effector	475:482	arg1	function					484:491	its effector function	471:491	its effector function	471:491	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	9	14	theme	aberrant	1670:1677	arg1	N-glycosylation					1689:1703	aberrant serum IgG N-glycosylation	1670:1703	aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma	1670:1782	Using this strategy, aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma was revealed.					
32110292	7	15	theme	high-energy	1302:1312	arg1	dissociation					1326:1337	high-energy collisional dissociation	1302:1337	high-energy collisional dissociation	1302:1337	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	3	16	theme	amino	678:682	arg1	sequence					689:696	a highly similar amino acid sequence	661:696	a highly similar amino acid sequence	661:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	3	17	theme	heterogeneous	514:526	arg1	IgG					503:505	IgG	503:505	IgG highly heterogeneous	503:526	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	6	18	gly	N-glycopeptides	1246:1260	arg2	N-glycopeptides					1246:1260	N-glycopeptides	1246:1260	N-glycopeptides	1246:1260	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	9	19	theme	IgG	1685:1687	arg1	N-glycosylation					1689:1703	aberrant serum IgG N-glycosylation	1670:1703	aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma	1670:1782	Using this strategy, aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma was revealed.					
32110292	6	20	theme	dissociation	1141:1152	arg1	efficiency					1154:1163	the electron transfer dissociation efficiency	1119:1163	the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1119:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	10	21	theme	site-specific	1970:1982	arg1	profiling					1984:1992	large-scale site-specific profiling	1958:1992	large-scale site-specific profiling of glycoproteomes	1958:2010	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	6	22	theme	sequence	1225:1232	arg1	coverage					1234:1241	a 100% peptide sequence coverage	1210:1241	a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1210:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	4	23	from	glycosylation	814:826	arg1	mAbs					831:834	mAbs	831:834	mAbs	831:834	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	6	24	theme	electron	1123:1130	arg1	efficiency					1154:1163	the electron transfer dissociation efficiency	1119:1163	the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1119:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	6	25	theme	%	1215:1215	arg1	coverage					1234:1241	a 100% peptide sequence coverage	1210:1241	a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1210:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	1	26	from	one	137:139	arg1	system					202:207	the immune system	191:207	the immune system	191:207	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	9	27	theme	IgG	1714:1716	arg1	subclasses					1718:1727	four IgG subclasses	1709:1727	four IgG subclasses associated with cirrhosis and hepatocellular carcinoma	1709:1782	Using this strategy, aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma was revealed.					
32110292	6	28	from	efficiency	1154:1163	arg1	analysis					1186:1193	mass spectrometry analysis	1168:1193	mass spectrometry analysis	1168:1193	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	2	29	theme	therapeutic	400:410	arg1	mAbs					412:415	most therapeutic mAbs	395:415	most therapeutic mAbs	395:415	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	5	30	theme	protein	1046:1052	arg1	sequence					1054:1061	the protein sequence	1042:1061	the protein sequence	1042:1061	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	1	31	gly	glycoproteins	174:186	arg1	glycoproteins					174:186	glycoproteins	174:186	glycoproteins	174:186	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	4	32	gly	glycosylation	814:826	arg1	mAbs					831:834	mAbs	831:834	mAbs	831:834	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	6	33	theme	IgG	1283:1285	arg1	subclasses					1269:1278	all subclasses	1265:1278	all subclasses of IgG	1265:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	6	34	theme	labeling	1090:1097	arg1	strategy					1099:1106	a chemical labeling strategy	1079:1106	a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1079:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	7	35	gly	N-glycosylation	1393:1407	arg1	IgG					1420:1422	IgG	1420:1422	IgG	1420:1422	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	9	36	theme	hepatocellular	1759:1772	arg1	carcinoma					1774:1782	hepatocellular carcinoma	1759:1782	hepatocellular carcinoma	1759:1782	Using this strategy, aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma was revealed.					
32110292	8	37	theme	glycosylation	1456:1468	arg1	analysis					1470:1477	comprehensive glycosylation analysis	1442:1477	This comprehensive glycosylation analysis strategy for the first time	1437:1505	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	5	38	theme	IgG	906:908	arg1	subclasses					910:919	IgG subclasses	906:919	IgG subclasses	906:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	0	39	theme	Chemical	0:7	arg1	labeling					9:16	Chemical labeling	0:16	Chemical labeling for fine mapping of IgG N-glycosylation by ETD-MS.	0:67	Chemical labeling for fine mapping of IgG N-glycosylation by ETD-MS.					
32110292	5	40	theme	site-specific	846:858	arg1	analysis					894:901	site-specific and comprehensive N-glycosylation analysis	846:901	site-specific and comprehensive N-glycosylation analysis of IgG subclasses	846:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	5	41	theme	comprehensive	864:876	arg1	analysis					894:901	site-specific and comprehensive N-glycosylation analysis	846:901	site-specific and comprehensive N-glycosylation analysis of IgG subclasses	846:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	3	42	gly	N-glycosylation	618:632	arg2	site					634:637	only a single N-glycosylation site	604:637	only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence	604:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	10	43	theme	more	1839:1842	arg1	times					1833:1837	5 times	1831:1837	5 times more intact glycopeptides from human serum than the native-ETD method	1831:1907	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	4	44	theme	IgG	726:728	arg1	glycosylation					730:742	IgG glycosylation	726:742	IgG glycosylation	726:742	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	0	45	theme	IgG	38:40	arg1	N-glycosylation					42:56	IgG N-glycosylation	38:56	IgG N-glycosylation	38:56	Chemical labeling for fine mapping of IgG N-glycosylation by ETD-MS.					
32110292	6	46	from	coverage	1234:1241	arg1	subclasses					1269:1278	all subclasses	1265:1278	all subclasses of IgG	1265:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	8	47	gly	N-glycopeptides	1557:1571	arg2	N-glycopeptides					1557:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	3	48	theme	IgG	592:594	arg1	subclasses					578:587	all four subclasses	569:587	all four subclasses of IgG	569:594	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	2	49	theme	steady	260:265	arg1	increase					267:274	a steady increase	258:274	a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases	258:375	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	7	50	theme	IgG	1420:1422	arg1	profile					1409:1415	the N-glycosylation profile	1389:1415	the N-glycosylation profile of IgG	1389:1422	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	5	51	dep	coverage	986:993	arg1	the					965:967	the	965:967	the	965:967	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	1	52	contain	contains	99:106	arg2	IgG1-4					125:130	IgG1-4	125:130	IgG1-4	125:130	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	52	contain	contains	99:106	arg1	IgG					87:89	IgG	87:89	IgG	87:89	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	52	contain	contains	99:106	arg2	subclasses					113:122	four subclasses	108:122	four subclasses (IgG1-4)	108:131	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	52	contain	contains	99:106	arg1	G					84:84	Immunoglobulin G	69:84	Immunoglobulin G (IgG)	69:90	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	6	53	theme	100	1212:1214	arg1	%					1215:1215	%	1215:1215	%	1215:1215	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	1	54	theme	immune	195:200	arg1	system					202:207	the immune system	191:207	the immune system	191:207	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	7	55	theme	profile	1409:1415	arg1	mapping					1378:1384	fine mapping	1373:1384	fine mapping of the N-glycosylation profile of IgG	1373:1422	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	4	56	theme	IgG	779:781	arg1	function					783:790	the IgG function	775:790	the IgG function	775:790	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	7	57	theme	fine	1373:1376	arg1	mapping					1378:1384	fine mapping	1373:1384	fine mapping of the N-glycosylation profile of IgG	1373:1422	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	10	58	dep	more	1839:1842	arg1	glycopeptides					1851:1863	intact glycopeptides	1844:1863	intact glycopeptides from human serum	1844:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	3	59	theme	IgG	452:454	arg1	N-Glycosylation					433:447	N-Glycosylation	433:447	N-Glycosylation of IgG	433:454	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	4	60	theme	glycosylation	730:742	arg1	mapping					715:721	fine mapping	710:721	fine mapping of IgG glycosylation	710:742	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	7	61	theme	glycans	1364:1370	arg1	fragmentation					1347:1359	the fragmentation	1343:1359	the fragmentation of glycans	1343:1370	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	6	62	theme	spectrometry	1173:1184	arg1	analysis					1186:1193	mass spectrometry analysis	1168:1193	mass spectrometry analysis	1168:1193	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	10	63	gly	glycopeptides	1851:1863	arg2	glycopeptides					1851:1863	intact glycopeptides	1844:1863	intact glycopeptides from human serum	1844:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	10	63	gly	glycopeptides	1851:1863	arg1	serum					1876:1880	human serum	1870:1880	human serum	1870:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	2	64	theme	immune	243:248	arg1	system					250:255	the immune system	239:255	the immune system	239:255	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	10	65	theme	human	1870:1874	arg1	serum					1876:1880	human serum	1870:1880	human serum	1870:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	5	66	gly	glycosylation	1025:1037	arg1	sequence					1054:1061	the protein sequence	1042:1061	the protein sequence	1042:1061	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	6	67	theme	N-glycopeptides	1246:1260	arg1	coverage					1234:1241	a 100% peptide sequence coverage	1210:1241	a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1210:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	3	68	dep	structure	536:544	arg1	both					528:531	both	528:531	both	528:531	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	9	69	theme	serum	1679:1683	arg1	N-glycosylation					1689:1703	aberrant serum IgG N-glycosylation	1670:1703	aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma	1670:1782	Using this strategy, aberrant serum IgG N-glycosylation for four IgG subclasses associated with cirrhosis and hepatocellular carcinoma was revealed.					
32110292	7	70	theme	collisional	1314:1324	arg1	dissociation					1326:1337	high-energy collisional dissociation	1302:1337	high-energy collisional dissociation	1302:1337	Combined with high-energy collisional dissociation for the fragmentation of glycans, fine mapping of the N-glycosylation profile of IgG is achieved.					
32110292	3	71	theme	acid	684:687	arg1	sequence					689:696	a highly similar amino acid sequence	661:696	a highly similar amino acid sequence	661:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	6	72	theme	transfer	1132:1139	arg1	efficiency					1154:1163	the electron transfer dissociation efficiency	1119:1163	the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1119:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	3	73	theme	similar	670:676	arg1	sequence					689:696	a highly similar amino acid sequence	661:696	a highly similar amino acid sequence	661:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	6	74	theme	peptide	1217:1223	arg1	coverage					1234:1241	a 100% peptide sequence coverage	1210:1241	a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG	1210:1285	We report here a chemical labeling strategy to improve the electron transfer dissociation efficiency in mass spectrometry analysis, which enables a 100% peptide sequence coverage of N-glycopeptides in all subclasses of IgG.					
32110292	4	75	theme	aberrant	805:812	arg1	glycosylation					814:826	aberrant glycosylation	805:826	aberrant glycosylation in mAbs	805:834	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	5	76	theme	partial	969:975	arg1	coverage					986:993	partial sequence coverage	969:993	partial sequence coverage	969:993	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	8	77	with	discrimination	1518:1531	arg1	similarity					1583:1592	high similarity	1578:1592	high similarity in sequence	1578:1604	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	2	78	from	importance	225:234	arg1	system					250:255	the immune system	239:255	the immune system	239:255	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	5	79	gly	N-glycosylation	878:892	arg1	subclasses					910:919	IgG subclasses	906:919	IgG subclasses	906:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	3	80	contain	contain	596:602	arg1	subclasses					578:587	all four subclasses	569:587	all four subclasses of IgG	569:594	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	3	80	contain	contain	596:602	arg2	site					634:637	only a single N-glycosylation site	604:637	only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence	604:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	10	81	theme	glycoproteomes	1997:2010	arg1	profiling					1984:1992	large-scale site-specific profiling	1958:1992	large-scale site-specific profiling of glycoproteomes	1958:2010	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	2	82	theme	most	395:398	arg1	mAbs					412:415	most therapeutic mAbs	395:415	most therapeutic mAbs	395:415	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	8	83	theme	comprehensive	1442:1454	arg1	analysis					1470:1477	comprehensive glycosylation analysis	1442:1477	This comprehensive glycosylation analysis strategy for the first time	1437:1505	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	5	84	theme	sequence	1054:1061	arg1	glycosylation					1025:1037	glycosylation	1025:1037	glycosylation of the protein sequence	1025:1061	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	5	85	theme	N-glycosylation	878:892	arg1	analysis					894:901	site-specific and comprehensive N-glycosylation analysis	846:901	site-specific and comprehensive N-glycosylation analysis of IgG subclasses	846:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	8	86	theme	analysis	1470:1477	arg1	strategy					1479:1486	This comprehensive glycosylation analysis strategy	1437:1486	This comprehensive glycosylation analysis strategy for the first time	1437:1505	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	3	87	gly	N-Glycosylation	433:447	arg1	IgG					452:454	IgG	452:454	IgG	452:454	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	5	88	theme	connections	1007:1017	arg1	coverage					986:993	partial sequence coverage	969:993	partial sequence coverage	969:993	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	5	88	theme	connections	1007:1017	arg1	loss					999:1002	loss	999:1002	loss	999:1002	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	2	89	theme	biomarker	313:321	arg1	agent					341:345	the biomarker or biotherapeutic agent	309:345	agent	341:345	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	2	89	theme	biomarker	313:321	arg1	IgG					302:304	IgG	302:304	IgG	302:304	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	10	90	theme	native-ETD	1891:1900	arg1	method					1902:1907	the native-ETD method	1887:1907	the native-ETD method	1887:1907	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	3	91	with	region	649:654	arg1	sequence					689:696	a highly similar amino acid sequence	661:696	a highly similar amino acid sequence	661:696	N-Glycosylation of IgG is crucial for its effector function and makes IgG highly heterogeneous both in structure and function, although all four subclasses of IgG contain only a single N-glycosylation site in the Fc region with a highly similar amino acid sequence.					
32110292	2	92	theme	diseases	368:375	arg1	treatment					355:363	the treatment	351:363	the treatment of diseases	351:375	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	8	93	theme	first	1496:1500	arg1	time					1502:1505	the first time	1492:1505	the first time	1492:1505	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	0	94	theme	fine	22:25	arg1	mapping					27:33	fine mapping	22:33	fine mapping of IgG N-glycosylation	22:56	Chemical labeling for fine mapping of IgG N-glycosylation by ETD-MS.					
32110292	10	95	theme	intact	1844:1849	arg1	glycopeptides					1851:1863	intact glycopeptides	1844:1863	intact glycopeptides from human serum	1844:1880	Moreover, this method identifies 5 times more intact glycopeptides from human serum than the native-ETD method, implying that the approach can also accommodate large-scale site-specific profiling of glycoproteomes.					
32110292	1	96	theme	important	153:161	arg1	classes					163:169	the most important classes	144:169	the most important classes of glycoproteins	144:186	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	4	97	theme	fine	710:713	arg1	mapping					715:721	fine mapping	710:721	fine mapping of IgG glycosylation	710:742	Therefore, fine mapping of IgG glycosylation is necessary for understanding the IgG function and avoiding aberrant glycosylation in mAbs.					
32110292	2	98	theme	biotherapeutic	326:339	arg1	agent					341:345	the biomarker or biotherapeutic agent	309:345	agent	341:345	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	2	98	theme	biotherapeutic	326:339	arg1	IgG					302:304	IgG	302:304	IgG	302:304	Because of its importance in the immune system, a steady increase of interest in developing IgG as the biomarker or biotherapeutic agent for the treatment of diseases has been seen, as most therapeutic mAbs were IgG-based.					
32110292	0	99	theme	N-glycosylation	42:56	arg1	mapping					27:33	fine mapping	22:33	fine mapping of IgG N-glycosylation	22:56	Chemical labeling for fine mapping of IgG N-glycosylation by ETD-MS.					
32110292	8	100	theme	IgG3	1536:1539	arg1	N-glycopeptides					1557:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	5	101	theme	subclasses	910:919	arg1	analysis					894:901	site-specific and comprehensive N-glycosylation analysis	846:901	site-specific and comprehensive N-glycosylation analysis of IgG subclasses	846:919	However, site-specific and comprehensive N-glycosylation analysis of IgG subclasses still cannot be achieved by MS alone due to the partial sequence coverage and loss of connections among glycosylation of the protein sequence.					
32110292	8	102	theme	IgG4	1545:1548	arg1	N-glycopeptides					1557:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	1	103	theme	Immunoglobulin	69:82	arg1	IgG					87:89	IgG	87:89	IgG	87:89	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	103	theme	Immunoglobulin	69:82	arg1	G					84:84	Immunoglobulin G	69:84	Immunoglobulin G (IgG)	69:90	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	8	104	theme	antibody-based	1618:1631	arg1	pre-separation					1633:1646	the antibody-based pre-separation	1614:1646	the antibody-based pre-separation	1614:1646	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	1	105	theme	glycoproteins	174:186	arg1	classes					163:169	the most important classes	144:169	the most important classes of glycoproteins	144:186	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	106	theme	classes	163:169	arg1	one					137:139	one	137:139	one	137:139	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	1	106	theme	classes	163:169	arg1	classes					163:169	the most important classes	144:169	the most important classes of glycoproteins	144:186	Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important classes of glycoproteins in the immune system.					
32110292	8	107	theme	N-glycopeptides	1557:1571	arg1	discrimination					1518:1531	the discrimination	1514:1531	the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence	1514:1604	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
32110292	8	108	theme	intact	1550:1555	arg1	N-glycopeptides					1557:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	IgG3 and IgG4 intact N-glycopeptides	1536:1571	This comprehensive glycosylation analysis strategy for the first time allows the discrimination of IgG3 and IgG4 intact N-glycopeptides with high similarity in sequence without the antibody-based pre-separation.					
30974135	7	0	theme	superabsorbent	1160:1173	arg1	hydrogel					1175:1182	superabsorbent hydrogel	1160:1182	superabsorbent hydrogel	1160:1182	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	3	1	theme	polymer	534:540	arg1	composite					542:550	the as-prepared polymer composite	518:550	the as-prepared polymer composite	518:550	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	0	2	theme	waste	15:19	arg1	Utilization					0:10	Utilization	0:10	Utilization of waste	0:19	Utilization of waste hemicelluloses lye for superabsorbent hydrogel synthesis.					
30974135	6	3	theme	PVA	1057:1059	arg1	effect					1047:1052	the synergy effect	1035:1052	the synergy effect of PVA and bentonite	1035:1073	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	7	4	theme	hydrogel	1175:1182	arg1	structure					1147:1155	the new structure	1139:1155	the new structure of superabsorbent hydrogel	1139:1182	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	3	5	theme	composite	542:550	arg1	properties					504:513	the swelling properties	491:513	the swelling properties of the as-prepared polymer composite	491:550	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	7	6	theme	engineering	1242:1252	arg1	processes					1254:1262	green and sustainable chemical engineering processes	1211:1262	green and sustainable chemical engineering processes	1211:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	6	7	theme	adsorption	965:974	arg1	kinetics					976:983	The adsorption kinetics	961:983	The adsorption kinetics	961:983	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	5	8	dep	hemicelluloses-g-AA/bentonite	828:856	arg1	hydrogels					912:920	hydrogels	912:920	hydrogels	912:920	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	9	theme	water	759:763	arg1	rate					776:779	water absorption rate	759:779	water absorption rate	759:779	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	7	10	from	lye	1204:1206	arg1	processes					1254:1262	green and sustainable chemical engineering processes	1211:1262	green and sustainable chemical engineering processes	1211:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	6	11	theme	synergy	1039:1045	arg1	effect					1047:1052	the synergy effect	1035:1052	the synergy effect of PVA and bentonite	1035:1073	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	5	12	theme	absorption	765:774	arg1	rate					776:779	water absorption rate	759:779	water absorption rate	759:779	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	13	theme	hemicelluloses-g-AA/bentonite-PVA	868:900	arg1	reusability					813:823	water reusability	807:823	water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels	807:920	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	13	theme	hemicelluloses-g-AA/bentonite-PVA	868:900	arg1	rate					776:779	water absorption rate	759:779	water absorption rate	759:779	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	13	theme	hemicelluloses-g-AA/bentonite-PVA	868:900	arg1	retainability					788:800	water retainability	782:800	water retainability	782:800	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	13	theme	hemicelluloses-g-AA/bentonite-PVA	868:900	arg1	absorbency					707:716	liquid absorbency	700:716	liquid absorbency in distilled water and saline solutions	700:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	7	14	theme	chemical	1233:1240	arg1	processes					1254:1262	green and sustainable chemical engineering processes	1211:1262	green and sustainable chemical engineering processes	1211:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	7	15	theme	new	1143:1145	arg1	structure					1147:1155	the new structure	1139:1155	the new structure of superabsorbent hydrogel	1139:1182	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	1	16	theme	high-performance	81:96	arg1	hydrogel					113:120	A high-performance superabsorbent hydrogel	79:120	A high-performance superabsorbent hydrogel	79:120	A high-performance superabsorbent hydrogel have been successfully fabricated by using waste hemicelluloses lye.					
30974135	4	17	theme	composite	679:687	arg1	morphologies					605:616	the morphologies	601:616	the morphologies	601:616	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	4	17	theme	composite	679:687	arg1	stability					642:650	thermal stability	634:650	thermal stability of as-synthesized hydrogel composite	634:687	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	4	17	theme	composite	679:687	arg1	structure					619:627	structure	619:627	structure	619:627	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	5	18	theme	hemicelluloses-g-AA/bentonite	828:856	arg1	reusability					813:823	water reusability	807:823	water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels	807:920	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	18	theme	hemicelluloses-g-AA/bentonite	828:856	arg1	rate					776:779	water absorption rate	759:779	water absorption rate	759:779	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	18	theme	hemicelluloses-g-AA/bentonite	828:856	arg1	retainability					788:800	water retainability	782:800	water retainability	782:800	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	18	theme	hemicelluloses-g-AA/bentonite	828:856	arg1	absorbency					707:716	liquid absorbency	700:716	liquid absorbency in distilled water and saline solutions	700:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	1	19	theme	superabsorbent	98:111	arg1	hydrogel					113:120	A high-performance superabsorbent hydrogel	79:120	A high-performance superabsorbent hydrogel	79:120	A high-performance superabsorbent hydrogel have been successfully fabricated by using waste hemicelluloses lye.					
30974135	7	20	theme	green	1211:1215	arg1	processes					1254:1262	green and sustainable chemical engineering processes	1211:1262	green and sustainable chemical engineering processes	1211:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	7	21	theme	waste	1198:1202	arg1	lye					1204:1206	the waste lye	1194:1206	the waste lye in green and sustainable chemical engineering processes	1194:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	5	22	from	rate	776:779	arg1	water					731:735	distilled water	721:735	distilled water	721:735	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	22	from	rate	776:779	arg1	solutions					748:756	saline solutions	741:756	saline solutions	741:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	7	23	theme	new	1118:1120	arg1	avenue					1122:1127	a new avenue	1116:1127	a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes	1116:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	3	24	theme	hemicelluloses-g-AA/bentonite	379:407	arg1	matrix					409:414	the hemicelluloses-g-AA/bentonite matrix	375:414	the hemicelluloses-g-AA/bentonite matrix	375:414	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	25	theme	polyvinyl	304:312	arg1	PVA					323:325	PVA	323:325	PVA	323:325	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	25	theme	polyvinyl	304:312	arg1	alcohol					314:320	polyvinyl alcohol	304:320	polyvinyl alcohol (PVA)	304:326	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	26	theme	swelling	495:502	arg1	properties					504:513	the swelling properties	491:513	the swelling properties of the as-prepared polymer composite	491:550	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	27	theme	semi-interpenetrating	427:447	arg1	semi-IPN					467:474	semi-IPN	467:474	semi-IPN	467:474	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	27	theme	semi-interpenetrating	427:447	arg1	networks					457:464	a semi-interpenetrating polymer networks	425:464	a semi-interpenetrating polymer networks (semi-IPN)	425:475	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	5	28	theme	saline	741:746	arg1	solutions					748:756	saline solutions	741:756	saline solutions	741:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	2	29	theme	extra	199:203	arg1	base					205:208	Not any extra base	191:208	Not any extra base	191:208	Not any extra base was added into the synthesis system for achieving hydrophilic polymer composite.					
30974135	4	30	theme	hydrogel	670:677	arg1	composite					679:687	as-synthesized hydrogel composite	655:687	as-synthesized hydrogel composite	655:687	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	3	31	theme	polymer	449:455	arg1	semi-IPN					467:474	semi-IPN	467:474	semi-IPN	467:474	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	3	31	theme	polymer	449:455	arg1	networks					457:464	a semi-interpenetrating polymer networks	425:464	a semi-interpenetrating polymer networks (semi-IPN)	425:475	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	5	32	theme	water	807:811	arg1	reusability					813:823	water reusability	807:823	water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels	807:920	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	6	33	theme	bentonite	1065:1073	arg1	effect					1047:1052	the synergy effect	1035:1052	the synergy effect of PVA and bentonite	1035:1073	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	4	34	theme	as-synthesized	655:668	arg1	composite					679:687	as-synthesized hydrogel composite	655:687	as-synthesized hydrogel composite	655:687	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	0	35	theme	hydrogel	59:66	arg1	synthesis					68:76	superabsorbent hydrogel synthesis	44:76	superabsorbent hydrogel synthesis	44:76	Utilization of waste hemicelluloses lye for superabsorbent hydrogel synthesis.					
30974135	6	36	theme	composites	1006:1015	arg1	kinetics					976:983	The adsorption kinetics	961:983	The adsorption kinetics	961:983	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	6	36	theme	composites	1006:1015	arg1	isotherms					989:997	isotherms	989:997	isotherms	989:997	The adsorption kinetics and isotherms of the composites were studied, and the synergy effect of PVA and bentonite were also proposed.					
30974135	5	37	theme	liquid	700:705	arg1	absorbency					707:716	liquid absorbency	700:716	liquid absorbency in distilled water and saline solutions	700:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	38	theme	water	782:786	arg1	retainability					788:800	water retainability	782:800	water retainability	782:800	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	0	39	theme	superabsorbent	44:57	arg1	synthesis					68:76	superabsorbent hydrogel synthesis	44:76	superabsorbent hydrogel synthesis	44:76	Utilization of waste hemicelluloses lye for superabsorbent hydrogel synthesis.					
30974135	5	40	from	retainability	788:800	arg1	water					731:735	distilled water	721:735	distilled water	721:735	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	40	from	retainability	788:800	arg1	solutions					748:756	saline solutions	741:756	saline solutions	741:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	2	41	theme	synthesis	229:237	arg1	system					239:244	the synthesis system	225:244	the synthesis system for achieving hydrophilic polymer composite	225:288	Not any extra base was added into the synthesis system for achieving hydrophilic polymer composite.					
30974135	3	42	theme	as-prepared	522:532	arg1	composite					542:550	the as-prepared polymer composite	518:550	the as-prepared polymer composite	518:550	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	7	43	theme	sustainable	1221:1231	arg1	processes					1254:1262	green and sustainable chemical engineering processes	1211:1262	green and sustainable chemical engineering processes	1211:1262	This method provides a new avenue to design the new structure of superabsorbent hydrogel and treat the waste lye in green and sustainable chemical engineering processes.					
30974135	5	44	theme	distilled	721:729	arg1	water					731:735	distilled water	721:735	distilled water	721:735	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	2	45	theme	polymer	272:278	arg1	composite					280:288	hydrophilic polymer composite	260:288	hydrophilic polymer composite	260:288	Not any extra base was added into the synthesis system for achieving hydrophilic polymer composite.					
30974135	1	46	theme	waste	165:169	arg1	lye					186:188	waste hemicelluloses lye	165:188	waste hemicelluloses lye	165:188	A high-performance superabsorbent hydrogel have been successfully fabricated by using waste hemicelluloses lye.					
30974135	4	47	theme	thermal	634:640	arg1	morphologies					605:616	the morphologies	601:616	the morphologies	601:616	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	4	47	theme	thermal	634:640	arg1	stability					642:650	thermal stability	634:650	thermal stability of as-synthesized hydrogel composite	634:687	SEM, FTIR, and TG were employed to characterize the morphologies, structure, and thermal stability of as-synthesized hydrogel composite.					
30974135	3	48	theme	reaction	342:349	arg1	system					351:356	the reaction system	338:356	the reaction system	338:356	In addition, polyvinyl alcohol (PVA) was added the reaction system to entrap within the hemicelluloses-g-AA/bentonite matrix and form a semi-interpenetrating polymer networks (semi-IPN) for enhancing the swelling properties of the as-prepared polymer composite.					
30974135	5	49	from	reusability	813:823	arg1	water					731:735	distilled water	721:735	distilled water	721:735	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	49	from	reusability	813:823	arg1	solutions					748:756	saline solutions	741:756	saline solutions	741:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	2	50	theme	hydrophilic	260:270	arg1	composite					280:288	hydrophilic polymer composite	260:288	hydrophilic polymer composite	260:288	Not any extra base was added into the synthesis system for achieving hydrophilic polymer composite.					
30974135	1	51	theme	hemicelluloses	171:184	arg1	lye					186:188	waste hemicelluloses lye	165:188	waste hemicelluloses lye	165:188	A high-performance superabsorbent hydrogel have been successfully fabricated by using waste hemicelluloses lye.					
30974135	5	52	from	absorbency	707:716	arg1	water					731:735	distilled water	721:735	distilled water	721:735	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
30974135	5	52	from	absorbency	707:716	arg1	solutions					748:756	saline solutions	741:756	saline solutions	741:756	Moreover, liquid absorbency in distilled water and saline solutions, water absorption rate, water retainability, and water reusability of hemicelluloses-g-AA/bentonite (HAB) and hemicelluloses-g-AA/bentonite-PVA (HAB-PVA) hydrogels were also investigated systematically.					
31815459	6	0	theme	1,2-cis-linked	1161:1174	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	8	1	theme	functional	1769:1778	arg1	interconversions					1786:1801	selective functional group interconversions	1759:1801	selective functional group interconversions	1759:1801	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	2	theme	repeating	1868:1876	arg1	tools					1904:1908	valuable tools	1895:1908	valuable tools for immunological evaluation and vaccine development	1895:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	2	theme	repeating	1868:1876	arg1	units					1878:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	7	3	theme	polysaccharide	1549:1562	arg1	unit					1510:1513	an aminopropyl linker-attached trisaccharide repeating unit	1455:1513	an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1455:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	4	theme	unreactive	1330:1339	arg1	4-OH					1347:1350	the most unreactive axial 4-OH	1321:1350	the most unreactive axial 4-OH of the rare disaccharide	1321:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	5	theme	aureus	1533:1538	arg1	polysaccharide					1549:1562	Staphylococcus aureus capsular polysaccharide	1518:1562	Staphylococcus aureus capsular polysaccharide type 5	1518:1569	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	5	theme	aureus	1533:1538	arg1	antigen					1598:1604	a potential antigen	1586:1604	a potential antigen for immunotherapy and vaccine development	1586:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	5	6	theme	glycoconjugate	890:903	arg1	vaccines					905:912	glycoconjugate vaccines	890:912	glycoconjugate vaccines	890:912	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	6	7	theme	repeating	1012:1020	arg1	unit					1022:1025	the conjugation-ready trisaccharide repeating unit	976:1025	the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	976:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	8	theme	repeating	1500:1508	arg1	unit					1510:1513	an aminopropyl linker-attached trisaccharide repeating unit	1455:1513	an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1455:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	0	9	theme	Capsular	130:137	arg1	Polysaccharide					139:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	7	10	theme	axial	1341:1345	arg1	4-OH					1347:1350	the most unreactive axial 4-OH	1321:1350	the most unreactive axial 4-OH of the rare disaccharide	1321:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	8	11	theme	immunological	1914:1926	arg1	evaluation					1928:1937	immunological evaluation	1914:1937	immunological evaluation	1914:1937	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	6	12	theme	conjugation-ready	980:996	arg1	unit					1022:1025	the conjugation-ready trisaccharide repeating unit	976:1025	the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	976:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	13	theme	linker-attached	1470:1484	arg1	unit					1510:1513	an aminopropyl linker-attached trisaccharide repeating unit	1455:1513	an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1455:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	14	theme	systematic	1241:1250	arg1	study					1252:1256	A systematic study	1239:1256	A systematic study	1239:1256	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	1	15	theme	critical	233:240	arg1	"					250:250	"critical priority"	232:250	three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	226:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	6	16	theme	total	957:961	arg1	synthesis					963:971	the first total synthesis	947:971	the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	947:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	17	with	glycosylation	1302:1314	arg1	4-OH					1347:1350	the most unreactive axial 4-OH	1321:1350	the most unreactive axial 4-OH of the rare disaccharide	1321:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	9	18	theme	related	2027:2033	arg1	glycans					2043:2049	other structurally related complex glycans	2008:2049	other structurally related complex glycans	2008:2049	The strategy is useful for the synthesis of other structurally related complex glycans.					
31815459	2	19	theme	infections	503:512	arg1	cause					475:479	a major cause	467:479	a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	467:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	19	theme	infections	503:512	arg1	pathogen					449:456	a "high priority" pathogen	431:456	a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	431:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	19	theme	infections	503:512	arg1	aureus					416:421	Staphylococcus aureus	401:421	Staphylococcus aureus	401:421	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	4	20	theme	unique	723:728	arg1	glycans					757:763	structurally unique and densely functionalized glycans	710:763	structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells	710:816	These pathogens express structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells.					
31815459	1	21	theme	bloodstream	363:373	arg1	infections					375:384	bloodstream infections	363:384	bloodstream infections	363:384	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	7	22	used	utilized	1409:1416	arg2	outcome					1397:1403	the successful outcome	1382:1403	the successful outcome	1382:1403	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	23	theme	difficult	1292:1300	arg1	glycosylation					1302:1314	the notoriously difficult glycosylation	1276:1314	the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide	1276:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	0	24	theme	Type	123:126	arg1	Polysaccharide					139:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	6	25	theme	l-fucosamine-	1119:1131	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	0	26	theme	Total	0:4	arg1	Syntheses					6:14	Total Syntheses	0:14	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.	0:177	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	2	27	theme	"	447:447	arg1	cause					475:479	a major cause	467:479	a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	467:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	27	theme	"	447:447	arg1	pathogen					449:456	a "high priority" pathogen	431:456	a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	431:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	27	theme	"	447:447	arg1	aureus					416:421	Staphylococcus aureus	401:421	Staphylococcus aureus	401:421	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	5	28	theme	valuable	850:857	arg1	antigens					837:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	5	28	theme	valuable	850:857	arg1	targets					859:865	valuable targets	850:865	valuable targets for the development of glycoconjugate vaccines and diagnostics	850:928	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	2	29	theme	serious	484:490	arg1	sepsis					534:539	sepsis	534:539	sepsis	534:539	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	29	theme	serious	484:490	arg1	infections					503:512	serious nosocomial infections	484:512	serious nosocomial infections such as bacteremia, sepsis, and endocarditis	484:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	29	theme	serious	484:490	arg1	bacteremia					522:531	bacteremia	522:531	bacteremia	522:531	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	29	theme	serious	484:490	arg1	endocarditis					546:557	endocarditis	546:557	endocarditis	546:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	30	theme	high	434:437	arg1	cause					475:479	a major cause	467:479	a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	467:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	30	theme	high	434:437	arg1	pathogen					449:456	a "high priority" pathogen	431:456	a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	431:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	30	theme	high	434:437	arg1	aureus					416:421	Staphylococcus aureus	401:421	Staphylococcus aureus	401:421	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	0	31	theme	Trisaccharide	37:49	arg1	Units					61:65	Conjugation-Ready Trisaccharide Repeating Units	19:65	Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	19:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	5	32	theme	Such	819:822	arg1	antigens					837:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	5	32	theme	Such	819:822	arg1	targets					859:865	valuable targets	850:865	valuable targets for the development of glycoconjugate vaccines and diagnostics	850:928	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	1	33	theme	deadly	337:342	arg1	pneumonia					390:398	pneumonia	390:398	pneumonia	390:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	1	33	theme	deadly	337:342	arg1	infections					375:384	bloodstream infections	363:384	bloodstream infections	363:384	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	1	33	theme	deadly	337:342	arg1	infections					344:353	severe and often deadly infections	320:353	infections	344:353	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	6	34	theme	regioselective	1203:1216	arg1	glycosylation					1218:1230	its regioselective glycosylation	1199:1230	its regioselective glycosylation at O3	1199:1236	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	9	35	theme	complex	2035:2041	arg1	glycans					2043:2049	other structurally related complex glycans	2008:2049	other structurally related complex glycans	2008:2049	The strategy is useful for the synthesis of other structurally related complex glycans.					
31815459	1	36	theme	priority	242:249	arg1	"					250:250	"critical priority"	232:250	three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	226:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	7	37	theme	rare	1359:1362	arg1	disaccharide					1364:1375	the rare disaccharide	1355:1375	the rare disaccharide	1355:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	1	38	theme	multi-drug-resistant	252:271	arg1	pathogens					273:281	three "critical priority" multi-drug-resistant pathogens	226:281	three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	226:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	7	39	theme	potential	1588:1596	arg1	polysaccharide					1549:1562	Staphylococcus aureus capsular polysaccharide	1518:1562	Staphylococcus aureus capsular polysaccharide type 5	1518:1569	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	39	theme	potential	1588:1596	arg1	antigen					1598:1604	a potential antigen	1586:1604	a potential antigen for immunotherapy and vaccine development	1586:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	6	40	theme	disaccharide	1176:1187	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	8	41	theme	complex	1830:1836	arg1	tools					1904:1908	valuable tools	1895:1908	valuable tools for immunological evaluation and vaccine development	1895:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	41	theme	complex	1830:1836	arg1	units					1878:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	6	42	theme	d-fucosamine-containing	1137:1159	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	8	43	theme	selective	1759:1767	arg1	interconversions					1786:1801	selective functional group interconversions	1759:1801	selective functional group interconversions	1759:1801	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	44	theme	trisaccharide	1854:1866	arg1	tools					1904:1908	valuable tools	1895:1908	valuable tools for immunological evaluation and vaccine development	1895:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	44	theme	trisaccharide	1854:1866	arg1	units					1878:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	45	theme	group	1780:1784	arg1	interconversions					1786:1801	selective functional group interconversions	1759:1801	selective functional group interconversions	1759:1801	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	0	46	theme	Units	61:65	arg1	Syntheses					6:14	Total Syntheses	0:14	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.	0:177	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	7	47	theme	capsular	1540:1547	arg1	polysaccharide					1549:1562	Staphylococcus aureus capsular polysaccharide	1518:1562	Staphylococcus aureus capsular polysaccharide type 5	1518:1569	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	47	theme	capsular	1540:1547	arg1	antigen					1598:1604	a potential antigen	1586:1604	a potential antigen for immunotherapy and vaccine development	1586:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	5	48	theme	vaccines	905:912	arg1	development					875:885	the development	871:885	the development of glycoconjugate vaccines and diagnostics	871:928	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	6	49	theme	unit	1022:1025	arg1	synthesis					963:971	the first total synthesis	947:971	the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	947:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	50	theme	Staphylococcus	1518:1531	arg1	polysaccharide					1549:1562	Staphylococcus aureus capsular polysaccharide	1518:1562	Staphylococcus aureus capsular polysaccharide type 5	1518:1569	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	50	theme	Staphylococcus	1518:1531	arg1	antigen					1598:1604	a potential antigen	1586:1604	a potential antigen for immunotherapy and vaccine development	1586:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	0	51	theme	Polysaccharide	139:152	arg1	Units					61:65	Conjugation-Ready Trisaccharide Repeating Units	19:65	Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	19:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	8	52	theme	valuable	1895:1902	arg1	tools					1904:1908	valuable tools	1895:1908	valuable tools for immunological evaluation and vaccine development	1895:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	52	theme	valuable	1895:1902	arg1	units					1878:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	6	53	theme	trisaccharide	998:1010	arg1	unit					1022:1025	the conjugation-ready trisaccharide repeating unit	976:1025	the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	976:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	54	theme	unit	1510:1513	arg1	synthesis					1442:1450	the total synthesis	1432:1450	the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1432:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	0	55	theme	Vaccine	158:164	arg1	Development					166:176	Vaccine Development	158:176	Vaccine Development	158:176	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	7	56	theme	trisaccharide	1486:1498	arg1	unit					1510:1513	an aminopropyl linker-attached trisaccharide repeating unit	1455:1513	an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1455:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	7	57	theme	aminopropyl	1458:1468	arg1	unit					1510:1513	an aminopropyl linker-attached trisaccharide repeating unit	1455:1513	an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1455:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	8	58	theme	vaccine	1943:1949	arg1	development					1951:1961	vaccine development	1943:1961	vaccine development	1943:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	7	59	dep	polysaccharide	1549:1562	arg1	type					1564:1567	type 5	1564:1569	Staphylococcus aureus capsular polysaccharide type 5	1518:1569	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	6	60	theme	stereoselective	1070:1084	arg1	assembly					1100:1107	a highly stereoselective and efficient assembly	1061:1107	a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1061:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	4	61	theme	functionalized	742:755	arg1	glycans					757:763	structurally unique and densely functionalized glycans	710:763	structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells	710:816	These pathogens express structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells.					
31815459	0	62	theme	aeruginosa	82:91	arg1	Polysaccharide					139:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	3	63	theme	any	613:615	arg1	antibiotics					617:627	almost any antibiotics	606:627	almost any antibiotics	606:627	Owing to their ability to adapt resistance to almost any antibiotics, vaccines against these pathogens are urgently required.					
31815459	5	64	theme	diagnostics	918:928	arg1	development					875:885	the development	871:885	the development of glycoconjugate vaccines and diagnostics	871:928	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	6	65	dep	Pseudomonas	1030:1040	arg1	aeruginosa					1042:1051	aeruginosa	1042:1051	aeruginosa	1042:1051	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	7	66	theme	total	1436:1440	arg1	synthesis					1442:1450	the total synthesis	1432:1450	the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development	1432:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	6	67	theme	O11	1053:1055	arg1	unit					1022:1025	the conjugation-ready trisaccharide repeating unit	976:1025	the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	976:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	2	68	theme	nosocomial	492:501	arg1	sepsis					534:539	sepsis	534:539	sepsis	534:539	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	68	theme	nosocomial	492:501	arg1	infections					503:512	serious nosocomial infections	484:512	serious nosocomial infections such as bacteremia, sepsis, and endocarditis	484:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	68	theme	nosocomial	492:501	arg1	bacteremia					522:531	bacteremia	522:531	bacteremia	522:531	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	68	theme	nosocomial	492:501	arg1	endocarditis					546:557	endocarditis	546:557	endocarditis	546:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	0	69	theme	aureus	116:121	arg1	Polysaccharide					139:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	70:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	6	70	theme	Pseudomonas	1030:1040	arg1	O11					1053:1055	Pseudomonas aeruginosa O11	1030:1055	Pseudomonas aeruginosa O11	1030:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	8	71	theme	judicious	1653:1661	arg1	selection					1663:1671	The judicious selection	1649:1671	The judicious selection of protecting groups and reaction conditions	1649:1716	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	6	72	gly	glycosylation	1218:1230	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	72	gly	glycosylation	1218:1230	arg1	O3					1235:1236	O3	1235:1236	O3	1235:1236	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	72	gly	glycosylation	1218:1230	arg2	O3					1235:1236	O3	1235:1236	O3	1235:1236	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	5	73	theme	carbohydrate	824:835	arg1	antigens					837:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens	819:844	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	5	73	theme	carbohydrate	824:835	arg1	targets					859:865	valuable targets	850:865	valuable targets for the development of glycoconjugate vaccines and diagnostics	850:928	Such carbohydrate antigens are valuable targets for the development of glycoconjugate vaccines and diagnostics.					
31815459	6	74	theme	rare	1114:1117	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	75	from	O3	1235:1236	arg1	assembly					1100:1107	a highly stereoselective and efficient assembly	1061:1107	a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1061:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	75	from	O3	1235:1236	arg1	glycosylation					1218:1230	its regioselective glycosylation	1199:1230	its regioselective glycosylation at O3	1199:1236	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	0	76	theme	Conjugation-Ready	19:35	arg1	Units					61:65	Conjugation-Ready Trisaccharide Repeating Units	19:65	Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	19:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	7	77	theme	successful	1386:1395	arg1	outcome					1397:1403	the successful outcome	1382:1403	the successful outcome	1382:1403	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	2	78	theme	priority	439:446	arg1	cause					475:479	a major cause	467:479	a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	467:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	78	theme	priority	439:446	arg1	pathogen					449:456	a "high priority" pathogen	431:456	a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	431:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	78	theme	priority	439:446	arg1	aureus					416:421	Staphylococcus aureus	401:421	Staphylococcus aureus	401:421	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	0	79	theme	Repeating	51:59	arg1	Units					61:65	Conjugation-Ready Trisaccharide Repeating Units	19:65	Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide	19:152	Total Syntheses of Conjugation-Ready Trisaccharide Repeating Units of Pseudomonas aeruginosa O11 and Staphylococcus aureus Type 5 Capsular Polysaccharide for Vaccine Development.					
31815459	1	80	theme	severe	320:325	arg1	pneumonia					390:398	pneumonia	390:398	pneumonia	390:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	1	80	theme	severe	320:325	arg1	infections					375:384	bloodstream infections	363:384	bloodstream infections	363:384	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	1	80	theme	severe	320:325	arg1	infections					344:353	severe and often deadly infections	320:353	infections	344:353	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	6	81	theme	efficient	1090:1098	arg1	assembly					1100:1107	a highly stereoselective and efficient assembly	1061:1107	a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1061:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	9	82	theme	other	2008:2012	arg1	glycans					2043:2049	other structurally related complex glycans	2008:2049	other structurally related complex glycans	2008:2049	The strategy is useful for the synthesis of other structurally related complex glycans.					
31815459	8	83	theme	reaction	1698:1705	arg1	conditions					1707:1716	reaction conditions	1698:1716	reaction conditions	1698:1716	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	7	84	theme	disaccharide	1364:1375	arg1	4-OH					1347:1350	the most unreactive axial 4-OH	1321:1350	the most unreactive axial 4-OH of the rare disaccharide	1321:1375	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	2	85	theme	major	469:473	arg1	cause					475:479	a major cause	467:479	a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	467:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	85	theme	major	469:473	arg1	pathogen					449:456	a "high priority" pathogen	431:456	a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis	431:557	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	2	85	theme	major	469:473	arg1	aureus					416:421	Staphylococcus aureus	401:421	Staphylococcus aureus	401:421	Staphylococcus aureus is also a "high priority" pathogen which is a major cause of serious nosocomial infections such as bacteremia, sepsis, and endocarditis.					
31815459	6	86	theme	first	951:955	arg1	synthesis					963:971	the first total synthesis	947:971	the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11	947:1055	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	87	link	1,2-cis-linked	1161:1174	arg1	motif					1189:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1112:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	4	88	from	glycans	757:763	arg1	surfaces					774:781	their surfaces	768:781	their surfaces which are absent on the host cells	768:816	These pathogens express structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells.					
31815459	7	89	theme	vaccine	1628:1634	arg1	development					1636:1646	vaccine development	1628:1646	vaccine development	1628:1646	A systematic study was conducted for the notoriously difficult glycosylation with the most unreactive axial 4-OH of the rare disaccharide, and the successful outcome was utilized to accomplish the total synthesis of an aminopropyl linker-attached trisaccharide repeating unit of Staphylococcus aureus capsular polysaccharide type 5, which is also a potential antigen for immunotherapy and vaccine development.					
31815459	9	90	theme	glycans	2043:2049	arg1	synthesis					1995:2003	the synthesis	1991:2003	the synthesis of other structurally related complex glycans	1991:2049	The strategy is useful for the synthesis of other structurally related complex glycans.					
31815459	8	91	theme	stereoselective	1730:1744	arg1	assembly					1746:1753	the stereoselective assembly	1726:1753	the stereoselective assembly	1726:1753	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	4	92	theme	host	807:810	arg1	cells					812:816	the host cells	803:816	the host cells	803:816	These pathogens express structurally unique and densely functionalized glycans on their surfaces which are absent on the host cells.					
31815459	1	93	theme	"	250:250	arg1	pathogens					273:281	three "critical priority" multi-drug-resistant pathogens	226:281	three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	226:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	6	94	theme	motif	1189:1193	arg1	assembly					1100:1107	a highly stereoselective and efficient assembly	1061:1107	a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif	1061:1193	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	6	94	theme	motif	1189:1193	arg1	glycosylation					1218:1230	its regioselective glycosylation	1199:1230	its regioselective glycosylation at O3	1199:1236	Here, we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudomonas aeruginosa O11 via a highly stereoselective and efficient assembly of a rare l-fucosamine- and d-fucosamine-containing 1,2-cis-linked disaccharide motif and its regioselective glycosylation at O3.					
31815459	8	95	theme	linker-attached	1838:1852	arg1	tools					1904:1908	valuable tools	1895:1908	valuable tools for immunological evaluation and vaccine development	1895:1961	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	8	95	theme	linker-attached	1838:1852	arg1	units					1878:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	the structurally complex linker-attached trisaccharide repeating units	1813:1882	The judicious selection of protecting groups and reaction conditions allowed the stereoselective assembly and selective functional group interconversions to access the structurally complex linker-attached trisaccharide repeating units, which are valuable tools for immunological evaluation and vaccine development.					
31815459	1	96	theme	pathogens	273:281	arg1	group					217:221	the group	213:221	the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	213:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
31815459	1	96	theme	pathogens	273:281	arg1	pathogens					273:281	three "critical priority" multi-drug-resistant pathogens	226:281	three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia	226:398	Pseudomonas aeruginosa belongs to the group of three "critical priority" multi-drug-resistant pathogens listed by WHO and is responsible for severe and often deadly infections such as bloodstream infections and pneumonia.					
29509696	0	0	theme	Sea	107:109	arg1	Cucumber					111:118	Sea Cucumber	107:118	Sea Cucumber	107:118	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	2	1	theme	raw	495:497	arg1	material					499:506	raw material	495:506	raw material of 16.26 mL/g	495:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	4	2	theme	monosaccharide	825:838	arg1	compositions					840:851	monosaccharide compositions	825:851	monosaccharide compositions	825:851	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	1	3	from	activity	225:232	arg1	cucumber					262:269	sea cucumber Phyllophorus proteus (PPP)	258:296	sea cucumber Phyllophorus proteus (PPP)	258:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	4	theme	in	204:205	arg1	activity					225:232	in vitro antioxidant activity	204:232	in vitro antioxidant activity	204:232	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	4	5	theme	only	973:976	arg1	Fuc					1034:1036	Fuc	1034:1036	Fuc	1034:1036	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	4	5	theme	only	973:976	arg1	GalNAc					1023:1028	GalNAc	1023:1028	GalNAc	1023:1028	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	4	5	theme	only	973:976	arg1	monosaccharide					978:991	only monosaccharide	973:991	only monosaccharide	973:991	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	7	6	theme	ABTS	1418:1421	arg1	radical					1423:1429	ABTS radical	1418:1429	ABTS radical	1418:1429	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	7	theme	papain	581:586	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	8	theme	±	393:393	arg1	%					399:399	6.44 ± 0.06%	388:399	6.44 ± 0.06% for PPP	388:407	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	3	9	theme	Sepharose	788:796	arg1	CL-6B					798:802	Sepharose CL-6B	788:802	Sepharose CL-6B	788:802	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	2	10	theme	extraction	523:532	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	5	11	theme	PPP	1059:1061	arg1	contents					1047:1054	Sulfate contents	1039:1054	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2	1039:1087	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively.					
29509696	4	12	dep	monosaccharide	978:991	arg1	the					969:971	the	969:971	the	969:971	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	7	13	theme	radical	1406:1412	arg1	activities					1313:1322	obvious activities	1305:1322	obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent	1305:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	14	theme	potential	1546:1554	arg1	value					1556:1560	potential value	1546:1560	potential value for antioxidation	1546:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	15	theme	radical	1386:1392	arg1	activities					1313:1322	obvious activities	1305:1322	obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent	1305:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	16	theme	°C	574:575	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	16	theme	°C	574:575	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	16	theme	°C	574:575	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	16	theme	°C	574:575	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	16	theme	°C	574:575	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	1	17	from	characterization	183:198	arg1	cucumber					262:269	sea cucumber Phyllophorus proteus (PPP)	258:296	sea cucumber Phyllophorus proteus (PPP)	258:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	7	18	theme	novel	1526:1530	arg1	agents					1532:1537	novel agents	1526:1537	novel agents having potential value for antioxidation	1526:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	18	theme	novel	1526:1530	arg1	polysaccharides					1477:1491	the polysaccharides	1473:1491	the polysaccharides from Phyllophorus proteus	1473:1517	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	19	with	conditions	361:370	arg1	yield					379:383	a yield	377:383	a yield of 6.44 ± 0.06% for PPP	377:407	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	20	theme	mL/g	517:520	arg1	material					499:506	raw material	495:506	raw material of 16.26 mL/g	495:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	21	theme	Extraction	437:446	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	0	22	dep	Phyllophorus	120:131	arg1	Optimization					27:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	22	dep	Phyllophorus	120:131	arg1	proteus					133:139	Phyllophorus proteus	120:139	Phyllophorus proteus	120:139	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	22	dep	Phyllophorus	120:131	arg1	Characterization					41:56	Characterization	41:56	Characterization	41:56	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	22	dep	Phyllophorus	120:131	arg1	Activity					74:81	Antioxidant Activity	62:81	Antioxidant Activity	62:81	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	6	23	contain	had	1177:1179	arg1	PPP-1a					1170:1175	PPP-1a	1170:1175	PPP-1a	1170:1175	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	6	23	contain	had	1177:1179	arg1	PPP					1162:1164	PPP	1162:1164	PPP	1162:1164	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	6	23	contain	had	1177:1179	arg2	viscosity					1219:1227	intrinsic viscosity	1209:1227	intrinsic viscosity	1209:1227	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	6	23	contain	had	1177:1179	arg2	weight					1198:1203	higher molecular weight	1181:1203	higher molecular weight	1181:1203	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	1	24	theme	antioxidant	213:223	arg1	activity					225:232	in vitro antioxidant activity	204:232	in vitro antioxidant activity	204:232	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	3	25	theme	FPA98Cl	776:782	arg1	columns					804:810	FPA98Cl and Sepharose CL-6B columns	776:810	FPA98Cl and Sepharose CL-6B columns	776:810	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	2	26	theme	%	609:609	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	26	theme	%	609:609	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	26	theme	%	609:609	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	26	theme	%	609:609	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	26	theme	%	609:609	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	1	27	from	optimization	169:180	arg1	cucumber					262:269	sea cucumber Phyllophorus proteus (PPP)	258:296	sea cucumber Phyllophorus proteus (PPP)	258:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	2	28	theme	h	461:461	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	28	theme	h	461:461	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	28	theme	h	461:461	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	28	theme	h	461:461	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	28	theme	h	461:461	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	3	29	with	PPP-1b	646:651	arg1	weights					678:684	molecular weights	668:684	molecular weights of 369.60, 41.73 and 57.76 kDa	668:715	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	0	30	theme	Extraction	16:25	arg1	Optimization					27:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	3	31	theme	kDa	713:715	arg1	weights					678:684	molecular weights	668:684	molecular weights of 369.60, 41.73 and 57.76 kDa	668:715	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	1	32	dep	in	204:205	arg1	vitro					207:211	vitro	207:211	vitro	207:211	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	7	33	contain	having	1539:1544	arg2	value					1556:1560	potential value	1546:1560	potential value for antioxidation	1546:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	33	contain	having	1539:1544	arg1	agents					1532:1537	novel agents	1526:1537	novel agents having potential value for antioxidation	1526:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	33	contain	having	1539:1544	arg1	polysaccharides					1477:1491	the polysaccharides	1473:1491	the polysaccharides from Phyllophorus proteus	1473:1517	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	0	34	theme	Enzyme-Assisted	0:14	arg1	Optimization					27:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	1	35	theme	present	323:329	arg1	study					331:335	the present study	319:335	the present study	319:335	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	7	36	theme	1,1-diphenyl-2-picrylhydrazyl	1338:1366	arg1	radical					1368:1374	scavenging 1,1-diphenyl-2-picrylhydrazyl radical	1327:1374	scavenging 1,1-diphenyl-2-picrylhydrazyl radical	1327:1374	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	4	37	theme	compositions	840:851	arg1	Analysis					813:820	Analysis	813:820	Analysis of monosaccharide compositions	813:851	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	1	38	theme	polysaccharides	237:251	arg1	optimization					169:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	38	theme	polysaccharides	237:251	arg1	activity					225:232	in vitro antioxidant activity	204:232	in vitro antioxidant activity	204:232	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	38	theme	polysaccharides	237:251	arg1	characterization					183:198	characterization	183:198	characterization	183:198	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	5	39	theme	PPP-2	1083:1087	arg1	contents					1047:1054	Sulfate contents	1039:1054	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2	1039:1087	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively.					
29509696	6	40	theme	higher	1181:1186	arg1	weight					1198:1203	higher molecular weight	1181:1203	higher molecular weight	1181:1203	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	2	41	theme	extraction	350:359	arg1	conditions					361:370	The optimal extraction conditions	338:370	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP	338:407	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	41	theme	extraction	350:359	arg1	follows					428:434	follows	428:434	follows	428:434	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	42	theme	6.83	540:543	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	42	theme	6.83	540:543	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	42	theme	6.83	540:543	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	42	theme	6.83	540:543	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	42	theme	6.83	540:543	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	43	theme	optimal	342:348	arg1	conditions					361:370	The optimal extraction conditions	338:370	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP	338:407	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	43	theme	optimal	342:348	arg1	follows					428:434	follows	428:434	follows	428:434	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	1	44	theme	sea	258:260	arg1	cucumber					262:269	sea cucumber Phyllophorus proteus (PPP)	258:296	sea cucumber Phyllophorus proteus (PPP)	258:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	2	45	theme	solvent	484:490	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	45	theme	solvent	484:490	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	45	theme	solvent	484:490	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	45	theme	solvent	484:490	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	45	theme	solvent	484:490	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	7	46	dep	exhibited	1295:1303	arg1	suggested					1458:1466	suggested	1458:1466	suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation	1458:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	47	theme	superoxide	1395:1404	arg1	radical					1406:1412	superoxide radical	1395:1412	superoxide radical	1395:1412	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	4	48	theme	acid	1017:1020	arg1	Fuc					1034:1036	Fuc	1034:1036	Fuc	1034:1036	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	4	48	theme	acid	1017:1020	arg1	GalNAc					1023:1028	GalNAc	1023:1028	GalNAc	1023:1028	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	4	48	theme	acid	1017:1020	arg1	PPP-2					997:1001	PPP-2	997:1001	PPP-2	997:1001	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	4	48	theme	acid	1017:1020	arg1	monosaccharide					978:991	only monosaccharide	973:991	only monosaccharide	973:991	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	2	49	theme	extraction	473:482	arg1	solvent					484:490	extraction solvent	473:490	extraction solvent	473:490	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	7	50	theme	radical	1423:1429	arg1	activities					1313:1322	obvious activities	1305:1322	obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent	1305:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	51	theme	hydroxyl	1377:1384	arg1	radical					1386:1392	hydroxyl radical	1377:1392	hydroxyl radical	1377:1392	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	52	dep	follows	428:434	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	52	dep	follows	428:434	arg1	pH					534:535	extraction pH	523:535	extraction pH of 6.83	523:543	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	52	dep	follows	428:434	arg1	concentration					588:600	papain concentration	581:600	papain concentration of 0.15%	581:609	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	52	dep	follows	428:434	arg1	ratio					464:468	ratio	464:468	ratio of extraction solvent to raw material of 16.26 mL/g	464:520	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	2	52	dep	follows	428:434	arg1	time					448:451	Extraction time	437:451	Extraction time of 2.89 h	437:461	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	1	53	from	cucumber	262:269	arg1	optimization					169:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	53	from	cucumber	262:269	arg1	polysaccharides					237:251	polysaccharides	237:251	polysaccharides from sea cucumber Phyllophorus proteus (PPP)	237:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	53	from	cucumber	262:269	arg1	activity					225:232	in vitro antioxidant activity	204:232	in vitro antioxidant activity	204:232	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	53	from	cucumber	262:269	arg1	characterization					183:198	characterization	183:198	characterization	183:198	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	6	54	theme	molecular	1188:1196	arg1	weight					1198:1203	higher molecular weight	1181:1203	higher molecular weight	1181:1203	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	4	55	theme	Fuc	962:964	arg1	PPP-1b					952:957	PPP-1b	952:957	PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc	952:1036	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	5	56	theme	PPP-1a	1064:1069	arg1	contents					1047:1054	Sulfate contents	1039:1054	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2	1039:1087	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively.					
29509696	6	57	theme	intrinsic	1209:1217	arg1	viscosity					1219:1227	intrinsic viscosity	1209:1227	intrinsic viscosity	1209:1227	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
29509696	3	58	theme	purified	618:625	arg1	fractions					627:635	Three purified fractions	612:635	Three purified fractions	612:635	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	3	58	theme	purified	618:625	arg1	PPP-1a					638:643	PPP-1a	638:643	PPP-1a	638:643	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	1	59	theme	Enzyme-assisted	142:156	arg1	optimization					169:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	0	60	theme	Antioxidant	62:72	arg1	Activity					74:81	Antioxidant Activity	62:81	Antioxidant Activity	62:81	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	5	61	theme	Sulfate	1039:1045	arg1	contents					1047:1054	Sulfate contents	1039:1054	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2	1039:1087	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively.					
29509696	7	62	theme	radical	1368:1374	arg1	activities					1313:1322	obvious activities	1305:1322	obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent	1305:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	1	63	theme	extraction	158:167	arg1	optimization					169:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization	142:180	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	4	64	theme	glucuronic	1006:1015	arg1	acid					1017:1020	glucuronic acid	1006:1020	glucuronic acid	1006:1020	Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc.					
29509696	5	65	theme	PPP-1b	1072:1077	arg1	contents					1047:1054	Sulfate contents	1039:1054	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2	1039:1087	Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively.					
29509696	1	66	dep	cucumber	262:269	arg1	PPP					293:295	PPP	293:295	PPP	293:295	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	1	66	dep	cucumber	262:269	arg1	proteus					284:290	Phyllophorus proteus	271:290	sea cucumber Phyllophorus proteus (PPP)	258:296	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study.					
29509696	7	67	theme	obvious	1305:1311	arg1	activities					1313:1322	obvious activities	1305:1322	obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent	1305:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	68	theme	exraction	546:554	arg1	temperature					556:566	exraction temperature	546:566	exraction temperature of 50 °C	546:575	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	7	69	theme	scavenging	1327:1336	arg1	radical					1368:1374	scavenging 1,1-diphenyl-2-picrylhydrazyl radical	1327:1374	scavenging 1,1-diphenyl-2-picrylhydrazyl radical	1327:1374	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	3	70	theme	CL-6B	798:802	arg1	columns					804:810	FPA98Cl and Sepharose CL-6B columns	776:810	FPA98Cl and Sepharose CL-6B columns	776:810	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	7	71	from	proteus	1511:1517	arg1	agents					1532:1537	novel agents	1526:1537	novel agents having potential value for antioxidation	1526:1578	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	7	71	from	proteus	1511:1517	arg1	polysaccharides					1477:1491	the polysaccharides	1473:1491	the polysaccharides from Phyllophorus proteus	1473:1517	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	0	72	theme	Polysaccharides	86:100	arg1	Optimization					27:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	72	theme	Polysaccharides	86:100	arg1	Characterization					41:56	Characterization	41:56	Characterization	41:56	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	72	theme	Polysaccharides	86:100	arg1	Activity					74:81	Antioxidant Activity	62:81	Antioxidant Activity	62:81	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	73	from	Cucumber	111:118	arg1	Optimization					27:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization	0:38	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	73	from	Cucumber	111:118	arg1	Characterization					41:56	Characterization	41:56	Characterization	41:56	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	0	73	from	Cucumber	111:118	arg1	Activity					74:81	Antioxidant Activity	62:81	Antioxidant Activity	62:81	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.					
29509696	3	74	theme	columns	804:810	arg1	chromatography					758:771	chromatography	758:771	chromatography of FPA98Cl and Sepharose CL-6B columns	758:810	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	7	75	from	activities	1313:1322	arg1	extent					1444:1449	different extent	1434:1449	different extent	1434:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	3	76	theme	molecular	668:676	arg1	weights					678:684	molecular weights	668:684	molecular weights of 369.60, 41.73 and 57.76 kDa	668:715	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	7	77	theme	different	1434:1442	arg1	extent					1444:1449	different extent	1434:1449	different extent	1434:1449	PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.					
29509696	2	78	theme	%	399:399	arg1	yield					379:383	a yield	377:383	a yield of 6.44 ± 0.06% for PPP	377:407	The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%.					
29509696	3	79	with	PPP-2	657:661	arg1	weights					678:684	molecular weights	668:684	molecular weights of 369.60, 41.73 and 57.76 kDa	668:715	Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns.					
29509696	6	80	dep	PPP-1b	1247:1252	arg1	the					1243:1245	the	1243:1245	the	1243:1245	PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2.					
30553331	5	0	theme	mesenteroides	1020:1032	arg1	proliferation					912:924	the proliferation	908:924	the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides	908:1032	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	1	1	theme	hot	178:180	arg1	LP-H					189:192	LP-H	189:192	LP-H	189:192	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	1	theme	hot	178:180	arg1	water					182:186	hot water	178:186	hot water (LP-H)	178:193	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	2	theme	enzymatic	252:260	arg1	treatments					262:271	grinding-assisted enzymatic treatments	234:271	grinding-assisted enzymatic treatments (LP-SE)	234:279	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	2	theme	enzymatic	252:260	arg1	LP-SE					274:278	LP-SE	274:278	LP-SE	274:278	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	0	3	from	properties	16:25	arg1	pulp					83:86	longan pulp	76:86	longan pulp	76:86	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	7	4	theme	superfine	1174:1181	arg1	treatment					1211:1219	superfine grinding-assisted enzymatic treatment	1174:1219	superfine grinding-assisted enzymatic treatment	1174:1219	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	5	5	theme	stronger	865:872	arg1	stimulation					874:884	stronger stimulation	865:884	stronger stimulation	865:884	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	0	6	theme	extraction	107:116	arg1	techniques					118:127	different extraction techniques	97:127	different extraction techniques	97:127	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	4	7	theme	-α-l-Araf-	786:795	arg1	linkage					734:740	specific glycosidic linkage	714:740	specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→	714:798	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	6	8	theme	chemical	1101:1108	arg1	composition					1110:1120	chemical composition	1101:1120	chemical composition	1101:1120	The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide.					
30553331	0	9	theme	different	97:105	arg1	techniques					118:127	different extraction techniques	97:127	different extraction techniques	97:127	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	5	10	theme	fermentum	994:1002	arg1	proliferation					912:924	the proliferation	908:924	the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides	908:1032	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	4	11	theme	1→	668:669	arg1	α-l-Rhap					675:682	α-l-Rhap	675:682	α-l-Rhap(l→	675:685	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	11	theme	1→	668:669	arg1	linkage					623:629	similar glycosidic linkage	604:629	similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	604:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	2	12	theme	polysaccharides	349:363	arg1	yields					286:291	The yields	282:291	The yields	282:291	The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated.					
30553331	2	12	theme	polysaccharides	349:363	arg1	properties					310:319	physicochemical properties	294:319	physicochemical properties	294:319	The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated.					
30553331	2	12	theme	polysaccharides	349:363	arg1	activities					335:344	prebiotic activities	325:344	prebiotic activities	325:344	The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated.					
30553331	4	13	theme	-β-d-Galp-	765:774	arg1	-α-l-Araf-					786:795	→4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→	745:798	-α-l-Araf-	786:795	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	3	14	theme	mannose	465:471	arg1	percentage					473:482	mannose percentage	465:482	mannose percentage	465:482	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	7	15	theme	great	1278:1282	arg1	potential					1284:1292	a great potential	1276:1292	a great potential	1276:1292	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	5	16	theme	Leuconostoc	1008:1018	arg1	mesenteroides					1020:1032	Leuconostoc mesenteroides	1008:1032	Leuconostoc mesenteroides	1008:1032	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	3	17	theme	particle	533:540	arg1	size					542:545	particle size	533:545	particle size	533:545	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	18	theme	LP-SE	487:491	arg1	arabinose					451:459	arabinose	451:459	arabinose	451:459	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	18	theme	LP-SE	487:491	arg1	percentage					473:482	mannose percentage	465:482	mannose percentage	465:482	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	18	theme	LP-SE	487:491	arg1	solubility					439:448	solubility	439:448	solubility	439:448	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	18	theme	LP-SE	487:491	arg1	yield					417:421	yield	417:421	yield	417:421	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	18	theme	LP-SE	487:491	arg1	content					430:436	sugar content	424:436	sugar content	424:436	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	0	19	from	activities	41:50	arg1	pulp					83:86	longan pulp	76:86	longan pulp	76:86	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	7	20	theme	prebiotic	1243:1251	arg1	activities					1253:1262	the highest prebiotic activities	1231:1262	the highest prebiotic activities	1231:1262	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	7	21	theme	grinding-assisted	1183:1199	arg1	treatment					1211:1219	superfine grinding-assisted enzymatic treatment	1174:1219	superfine grinding-assisted enzymatic treatment	1174:1219	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	3	22	theme	glucose	555:561	arg1	percentage					563:572	glucose percentage	555:572	glucose percentage	555:572	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	3	23	theme	sugar	424:428	arg1	content					430:436	sugar content	424:436	sugar content	424:436	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	0	24	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	1	25	dep	water	182:186	arg1	treatments					262:271	grinding-assisted enzymatic treatments	234:271	grinding-assisted enzymatic treatments (LP-SE)	234:279	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	25	dep	water	182:186	arg1	LP-SE					274:278	LP-SE	274:278	LP-SE	274:278	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	0	26	theme	prebiotic	31:39	arg1	activities					41:50	prebiotic activities	31:50	prebiotic activities	31:50	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	4	27	theme	-β-d-Glcp	748:756	arg1	-α-l-Araf-					786:795	→4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→	745:798	-α-l-Araf-	786:795	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	6	28	theme	polysaccharide	1139:1152	arg1	structure					1126:1134	structure	1126:1134	structure	1126:1134	The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide.					
30553331	6	28	theme	polysaccharide	1139:1152	arg1	composition					1110:1120	chemical composition	1101:1120	chemical composition	1101:1120	The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide.					
30553331	2	29	theme	physicochemical	294:308	arg1	properties					310:319	physicochemical properties	294:319	physicochemical properties	294:319	The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated.					
30553331	1	30	theme	grinding	205:212	arg1	superfine					196:203	superfine grinding	196:212	superfine grinding (LP-S)	196:219	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	30	theme	grinding	205:212	arg1	LP-S					215:218	LP-S	215:218	LP-S	215:218	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	4	31	dep	-α-l-Araf-	786:795	arg1	→5					783:784	→5	783:784	→5	783:784	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	31	dep	-α-l-Araf-	786:795	arg1	1→					776:777	1→	776:777	1→	776:777	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	7	32	theme	highest	1235:1241	arg1	activities					1253:1262	the highest prebiotic activities	1231:1262	the highest prebiotic activities	1231:1262	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	5	33	theme	bulgaricus	968:977	arg1	proliferation					912:924	the proliferation	908:924	the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides	908:1032	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	7	34	theme	medical	1329:1335	arg1	industry					1337:1344	medical industry	1329:1344	medical industry	1329:1344	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	4	35	theme	-α-l-Araf-	637:646	arg1	1→					668:669	→3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	634:669	1→	668:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	36	theme	-β-d-Galp-	657:666	arg1	1→					668:669	→3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	634:669	1→	668:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	0	37	from	pulp	83:86	arg1	polysaccharides					55:69	polysaccharides	55:69	polysaccharides from longan pulp	55:86	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	0	37	from	pulp	83:86	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	0	37	from	pulp	83:86	arg1	activities					41:50	prebiotic activities	31:50	prebiotic activities	31:50	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	1	38	theme	Longan	130:135	arg1	polysaccharides					142:156	Longan pulp polysaccharides	130:156	Longan pulp polysaccharides	130:156	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	0	39	theme	polysaccharides	55:69	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	0	39	theme	polysaccharides	55:69	arg1	activities					41:50	prebiotic activities	31:50	prebiotic activities	31:50	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	4	40	contain	contained	704:712	arg1	LP-S					809:812	LP-S	809:812	LP-S	809:812	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	40	contain	contained	704:712	arg1	LP-SE					818:822	LP-SE	818:822	LP-SE	818:822	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	40	contain	contained	704:712	arg2	they					694:697	they	694:697	they	694:697	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	40	contain	contained	704:712	arg2	linkage					734:740	specific glycosidic linkage	714:740	specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→	714:798	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	40	contain	contained	704:712	arg1	LP-H					803:806	LP-H	803:806	LP-H	803:806	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	40	contain	contained	704:712	arg1	they					694:697	they	694:697	they	694:697	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	6	41	dep	indicated	1047:1055	arg1	had					1082:1084	had	1082:1084	indicated three extraction methods had some effect on chemical composition and structure of polysaccharide	1047:1152	The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide.					
30553331	6	42	theme	extraction	1063:1072	arg1	methods					1074:1080	three extraction methods	1057:1080	three extraction methods	1057:1080	The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide.					
30553331	1	43	theme	pulp	137:140	arg1	polysaccharides					142:156	Longan pulp polysaccharides	130:156	Longan pulp polysaccharides	130:156	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	7	44	contain	have	1271:1274	arg2	potential					1284:1292	a great potential	1276:1292	a great potential	1276:1292	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	7	44	contain	have	1271:1274	arg1	activities					1253:1262	the highest prebiotic activities	1231:1262	the highest prebiotic activities	1231:1262	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	4	45	dep	α-l-Rhap	675:682	arg1	l→					684:685	l→	684:685	l→	684:685	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	46	theme	similar	604:610	arg1	linkage					623:629	similar glycosidic linkage	604:629	similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	604:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	47	theme	glycosidic	723:732	arg1	linkage					734:740	specific glycosidic linkage	714:740	specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→	714:798	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	5	48	theme	plantarum	943:951	arg1	proliferation					912:924	the proliferation	908:924	the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides	908:1032	Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides.					
30553331	7	49	theme	functional	1309:1318	arg1	food					1320:1323	functional food	1309:1323	functional food	1309:1323	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	2	50	theme	prebiotic	325:333	arg1	activities					335:344	prebiotic activities	325:344	prebiotic activities	325:344	The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated.					
30553331	4	51	contain	contained	594:602	arg2	linkage					623:629	similar glycosidic linkage	604:629	similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	604:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	51	contain	contained	594:602	arg2	α-l-Rhap					675:682	α-l-Rhap	675:682	α-l-Rhap(l→	675:685	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	4	51	contain	contained	594:602	arg1	LPs					590:592	Three LPs	584:592	Three LPs	584:592	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	3	52	theme	apparent	513:520	arg1	viscosity					522:530	its apparent viscosity	509:530	its apparent viscosity	509:530	Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined.					
30553331	0	53	theme	longan	76:81	arg1	pulp					83:86	longan pulp	76:86	longan pulp	76:86	Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques.					
30553331	4	54	theme	glycosidic	612:621	arg1	linkage					623:629	similar glycosidic linkage	604:629	similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→	604:669	Three LPs contained similar glycosidic linkage of →3)-α-l-Araf-(1→, →3,6)-β-d-Galp-(1→ and α-l-Rhap(l→, while they each contained specific glycosidic linkage of →4)-β-d-Glcp(l→, →4)-β-d-Galp-(1→ and →5)-α-l-Araf-(1→ in LP-H, LP-S and LP-SE, respectively.					
30553331	7	55	theme	enzymatic	1201:1209	arg1	treatment					1211:1219	superfine grinding-assisted enzymatic treatment	1174:1219	superfine grinding-assisted enzymatic treatment	1174:1219	LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.					
30553331	1	56	theme	grinding-assisted	234:250	arg1	treatments					262:271	grinding-assisted enzymatic treatments	234:271	grinding-assisted enzymatic treatments (LP-SE)	234:279	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
30553331	1	56	theme	grinding-assisted	234:250	arg1	LP-SE					274:278	LP-SE	274:278	LP-SE	274:278	Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE).					
29413573	0	0	from	analyses	24:31	arg1	control					44:50	quality control	36:50	quality control of Cassiae Semen polysaccharides	36:83	Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides.					
29413573	4	1	theme	polysaccharides	742:756	arg1	fingerprints					576:587	FT-IR fingerprints	570:587	FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA)	570:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	1	theme	polysaccharides	742:756	arg1	fingerprints					705:716	HPLC fingerprints	700:716	HPLC fingerprints of partially hydrolyzed polysaccharides with PCA	700:765	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	6	2	theme	multiple	1152:1159	arg1	methods					1174:1180	multiple chemometrics methods	1152:1180	multiple chemometrics methods	1152:1180	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	4	3	theme	hydrolyzed	731:740	arg1	polysaccharides					742:756	partially hydrolyzed polysaccharides	721:756	partially hydrolyzed polysaccharides with PCA	721:765	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	4	theme	authentic	811:819	arg1	source					821:826	the authentic source	807:826	the authentic source	807:826	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	5	5	theme	monosaccharide	1006:1019	arg1	composition					1021:1031	the monosaccharide composition	1002:1031	the monosaccharide composition	1002:1031	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	4	6	theme	correlation	637:647	arg1	analysis					649:656	Pearson correlation analysis	629:656	Pearson correlation analysis	629:656	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	7	theme	foreign	786:792	arg1	sources					794:800	the foreign sources	782:800	the foreign sources	782:800	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	8	with	polysaccharides	742:756	arg1	PCA					763:765	PCA	763:765	PCA	763:765	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	2	9	theme	polysaccharide	306:319	arg1	quality					321:327	the polysaccharide quality	302:327	the polysaccharide quality of Cassiae Semen harvested from different locations	302:379	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	4	10	theme	Pearson	629:635	arg1	analysis					649:656	Pearson correlation analysis	629:656	Pearson correlation analysis	629:656	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	5	11	theme	polysaccharides	887:901	arg1	fingerprints					849:860	HPLC or GC fingerprints	838:860	HPLC or GC fingerprints of completely hydrolyzed polysaccharides	838:901	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	4	12	theme	fingerprints	705:716	arg1	Analyses					558:565	Analyses	558:565	Analyses	558:565	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	1	13	theme	analytic	174:181	arg1	limitations					183:193	the analytic limitations	170:193	the analytic limitations	170:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	3	14	from	fingerprints	402:413	arg1	source					461:466	the authentic source	447:466	the authentic source	447:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	1	15	theme	Quality	86:92	arg1	issue					102:106	Quality control issue	86:106	Quality control issue	86:106	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	5	16	theme	GC	846:847	arg1	fingerprints					849:860	HPLC or GC fingerprints	838:860	HPLC or GC fingerprints of completely hydrolyzed polysaccharides	838:901	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	1	17	theme	control	94:100	arg1	issue					102:106	Quality control issue	86:106	Quality control issue	86:106	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	3	18	theme	authentic	451:459	arg1	source					461:466	the authentic source	447:466	the authentic source	447:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	6	19	theme	non/partially-hydrolyzed	1081:1104	arg1	polysaccharides					1106:1120	non/partially-hydrolyzed polysaccharides	1081:1120	non/partially-hydrolyzed polysaccharides	1081:1120	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	2	20	theme	Semen	340:344	arg1	quality					321:327	the polysaccharide quality	302:327	the polysaccharide quality of Cassiae Semen harvested from different locations	302:379	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	4	21	theme	HPLC	700:703	arg1	fingerprints					705:716	HPLC fingerprints	700:716	HPLC fingerprints of partially hydrolyzed polysaccharides with PCA	700:765	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	22	theme	fingerprints	576:587	arg1	Analyses					558:565	Analyses	558:565	Analyses	558:565	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	2	23	theme	Cassiae	332:338	arg1	Semen					340:344	Cassiae Semen	332:344	Cassiae Semen harvested from different locations	332:379	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	5	24	theme	hydrolyzed	876:885	arg1	polysaccharides					887:901	completely hydrolyzed polysaccharides	865:901	completely hydrolyzed polysaccharides	865:901	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	3	25	theme	GC	399:400	arg1	fingerprints					402:413	GC fingerprints	399:413	GC fingerprints	399:413	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	25	theme	GC	399:400	arg1	HPLC					389:392	HPLC	389:392	HPLC	389:392	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	25	theme	GC	399:400	arg1	profiles					497:504	standard profiles	488:504	standard profiles	488:504	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	25	theme	GC	399:400	arg1	FT-IR					382:386	FT-IR	382:386	FT-IR	382:386	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	4	26	theme	extracts	607:614	arg1	fingerprints					576:587	FT-IR fingerprints	570:587	FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA)	570:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	26	theme	extracts	607:614	arg1	fingerprints					705:716	HPLC fingerprints	700:716	HPLC fingerprints of partially hydrolyzed polysaccharides with PCA	700:765	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	6	27	theme	polysaccharides	1106:1120	arg1	fingerprints					1065:1076	FT-IR/HPLC fingerprints	1054:1076	FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods,	1054:1181	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	3	28	from	FT-IR	382:386	arg1	source					461:466	the authentic source	447:466	the authentic source	447:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	1	29	theme	potential	120:128	arg1	benefits					137:144	potential health benefits	120:144	potential health benefits of Cassiae Semen due to the analytic limitations	120:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	3	30	theme	foreign	541:547	arg1	sources					549:555	foreign sources	541:555	foreign sources	541:555	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	4	31	theme	polysaccharide	592:605	arg1	extracts					607:614	polysaccharide extracts	592:614	polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA)	592:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	2	32	theme	several	257:263	arg1	chemometrics					265:276	several chemometrics	257:276	several chemometrics	257:276	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	6	33	theme	chemometrics	1161:1172	arg1	methods					1174:1180	multiple chemometrics methods	1152:1180	multiple chemometrics methods	1152:1180	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	0	34	theme	quality	36:42	arg1	control					44:50	quality control	36:50	quality control of Cassiae Semen polysaccharides	36:83	Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides.					
29413573	5	35	theme	foreign	925:931	arg1	sources					933:939	all foreign sources	921:939	all foreign sources	921:939	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	3	36	from	HPLC	389:392	arg1	source					461:466	the authentic source	447:466	the authentic source	447:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	6	37	theme	Semen	1264:1268	arg1	sources					1245:1251	detecting and differentiating sources	1215:1251	detecting and differentiating sources of Cassiae Semen	1215:1268	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	3	38	theme	standard	488:495	arg1	fingerprints					402:413	GC fingerprints	399:413	GC fingerprints	399:413	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	38	theme	standard	488:495	arg1	HPLC					389:392	HPLC	389:392	HPLC	389:392	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	38	theme	standard	488:495	arg1	profiles					497:504	standard profiles	488:504	standard profiles	488:504	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	38	theme	standard	488:495	arg1	FT-IR					382:386	FT-IR	382:386	FT-IR	382:386	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	39	theme	polysaccharide	418:431	arg1	extracts					433:440	polysaccharide extracts	418:440	polysaccharide extracts from the authentic source	418:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	0	40	theme	Semen	63:67	arg1	polysaccharides					69:83	Cassiae Semen polysaccharides	55:83	Cassiae Semen polysaccharides	55:83	Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides.					
29413573	1	41	theme	health	130:135	arg1	benefits					137:144	potential health benefits	120:144	potential health benefits of Cassiae Semen due to the analytic limitations	120:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	3	42	theme	extracts	433:440	arg1	fingerprints					402:413	GC fingerprints	399:413	GC fingerprints	399:413	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	42	theme	extracts	433:440	arg1	profiles					497:504	standard profiles	488:504	standard profiles	488:504	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	42	theme	extracts	433:440	arg1	HPLC					389:392	HPLC	389:392	HPLC	389:392	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	42	theme	extracts	433:440	arg1	FT-IR					382:386	FT-IR	382:386	FT-IR	382:386	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	4	43	theme	FT-IR	570:574	arg1	fingerprints					576:587	FT-IR fingerprints	570:587	FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA)	570:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	2	44	theme	multiple-fingerprint	211:230	arg1	analysis					232:239	multiple-fingerprint analysis	211:239	multiple-fingerprint analysis integrated with several chemometrics	211:276	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	1	45	theme	due	163:165	arg1	Semen					157:161	Cassiae Semen	149:161	Cassiae Semen due to the analytic limitations	149:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	6	46	theme	differentiating	1229:1243	arg1	sources					1245:1251	detecting and differentiating sources	1215:1251	detecting and differentiating sources of Cassiae Semen	1215:1268	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	5	47	theme	HPLC	838:841	arg1	fingerprints					849:860	HPLC or GC fingerprints	838:860	HPLC or GC fingerprints of completely hydrolyzed polysaccharides	838:901	However, HPLC or GC fingerprints of completely hydrolyzed polysaccharides couldn't identify all foreign sources and the methodology using GC is quite limited in determining the monosaccharide composition.					
29413573	2	48	theme	different	361:369	arg1	locations					371:379	different locations	361:379	different locations	361:379	In this study, multiple-fingerprint analysis integrated with several chemometrics was performed to assess the polysaccharide quality of Cassiae Semen harvested from different locations.					
29413573	6	49	theme	Cassiae	1256:1262	arg1	Semen					1264:1268	Cassiae Semen	1256:1268	Cassiae Semen	1256:1268	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	6	50	theme	FT-IR/HPLC	1054:1063	arg1	fingerprints					1065:1076	FT-IR/HPLC fingerprints	1054:1076	FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods,	1054:1181	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	1	51	theme	Cassiae	149:155	arg1	Semen					157:161	Cassiae Semen	149:161	Cassiae Semen due to the analytic limitations	149:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	0	52	theme	polysaccharides	69:83	arg1	control					44:50	quality control	36:50	quality control of Cassiae Semen polysaccharides	36:83	Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides.					
29413573	3	53	from	source	461:466	arg1	fingerprints					402:413	GC fingerprints	399:413	GC fingerprints	399:413	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	53	from	source	461:466	arg1	extracts					433:440	polysaccharide extracts	418:440	polysaccharide extracts from the authentic source	418:466	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	53	from	source	461:466	arg1	HPLC					389:392	HPLC	389:392	HPLC	389:392	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	53	from	source	461:466	arg1	profiles					497:504	standard profiles	488:504	standard profiles	488:504	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	3	53	from	source	461:466	arg1	FT-IR					382:386	FT-IR	382:386	FT-IR	382:386	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	1	54	theme	Semen	157:161	arg1	benefits					137:144	potential health benefits	120:144	potential health benefits of Cassiae Semen due to the analytic limitations	120:193	Quality control issue overshadows potential health benefits of Cassiae Semen due to the analytic limitations.					
29413573	4	55	theme	component	671:679	arg1	PCA					691:693	PCA	691:693	PCA	691:693	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	55	theme	component	671:679	arg1	analysis					681:688	principal component analysis	661:688	principal component analysis (PCA)	661:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	0	56	theme	Cassiae	55:61	arg1	polysaccharides					69:83	Cassiae Semen polysaccharides	55:83	Cassiae Semen polysaccharides	55:83	Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides.					
29413573	6	57	theme	detecting	1215:1223	arg1	sources					1245:1251	detecting and differentiating sources	1215:1251	detecting and differentiating sources of Cassiae Semen	1215:1268	This indicates that FT-IR/HPLC fingerprints of non/partially-hydrolyzed polysaccharides, respectively, accompanied by multiple chemometrics methods, might be potentially applied in detecting and differentiating sources of Cassiae Semen.					
29413573	3	58	theme	sources	549:555	arg1	quality					530:536	the quality	526:536	the quality of foreign sources	526:555	FT-IR, HPLC, and GC fingerprints of polysaccharide extracts from the authentic source were established as standard profiles, applying to assess the quality of foreign sources.					
29413573	4	59	theme	principal	661:669	arg1	PCA					691:693	PCA	691:693	PCA	691:693	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
29413573	4	59	theme	principal	661:669	arg1	analysis					681:688	principal component analysis	661:688	principal component analysis (PCA)	661:694	Analyses of FT-IR fingerprints of polysaccharide extracts using either Pearson correlation analysis or principal component analysis (PCA), or HPLC fingerprints of partially hydrolyzed polysaccharides with PCA, distinguished the foreign sources from the authentic source.					
30013106	2	0	contain	contains	302:309	arg1	it					299:300	it	299:300	it	299:300	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	2	0	contain	contains	302:309	arg2	polysaccharides					311:325	polysaccharides	311:325	polysaccharides absent in humans	311:342	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	3	1	from	architecture	414:425	arg1	fungus					463:468	the pathogenic fungus	448:468	the pathogenic fungus Aspergillus fumigatus	448:490	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	3	2	theme	Aspergillus	470:480	arg1	fungus					463:468	the pathogenic fungus	448:468	the pathogenic fungus Aspergillus fumigatus	448:490	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	4	3	theme	glycosyl	568:575	arg1	analysis					585:592	glycosyl linkage analysis	568:592	glycosyl linkage analysis	568:592	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	6	4	theme	drug	1048:1051	arg1	response					1053:1060	drug response	1048:1060	drug response	1048:1060	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	4	5	theme	Solid-state	493:503	arg1	spectroscopy					509:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	6	6	theme	wall-targeted	1092:1104	arg1	antifungals					1106:1116	wall-targeted antifungals	1092:1116	wall-targeted antifungals	1092:1116	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	5	7	theme	cell	904:907	arg1	wall					909:912	cell wall rigidity and fungal virulence	904:942	wall	909:912	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	6	8	theme	cell	999:1002	arg1	walls					1004:1008	fungal cell walls	992:1008	fungal cell walls	992:1008	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	4	9	link	linked	714:719	arg1	β-glucans					721:729	diversely linked β-glucans	704:729	diversely linked β-glucans	704:729	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	6	10	theme	fungal	992:997	arg1	walls					1004:1008	fungal cell walls	992:1008	fungal cell walls	992:1008	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	1	11	dep	number	139:144	arg1	the					127:129	the	127:129	the	127:129	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	2	12	from	absent	327:332	arg1	humans					337:342	humans	337:342	humans	337:342	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	4	13	theme	hydrated	685:692	arg1	matrix					694:699	a hydrated matrix	683:699	a hydrated matrix of diversely linked β-glucans	683:729	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	4	14	theme	linkage	577:583	arg1	analysis					585:592	glycosyl linkage analysis	568:592	glycosyl linkage analysis	568:592	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	15	theme	fungal	104:109	arg1	infections					111:120	invasive fungal infections	95:120	invasive fungal infections	95:120	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	2	16	theme	cell	264:267	arg1	wall					269:272	The fungal cell wall	253:272	The fungal cell wall	253:272	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	2	16	theme	cell	264:267	arg1	target					289:294	a promising target	277:294	a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive	277:392	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	4	17	gly	glycoproteins	764:776	arg1	glycoproteins					764:776	glycoproteins	764:776	glycoproteins	764:776	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	2	18	from	humans	337:342	arg1	absent					327:332	absent	327:332	absent	327:332	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	2	19	theme	fungal	257:262	arg1	wall					269:272	The fungal cell wall	253:272	The fungal cell wall	253:272	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	2	19	theme	fungal	257:262	arg1	target					289:294	a promising target	277:294	a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive	277:392	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	3	20	theme	pathogenic	452:461	arg1	fungus					463:468	the pathogenic fungus	448:468	the pathogenic fungus Aspergillus fumigatus	448:490	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	2	21	theme	molecular	358:366	arg1	structure					368:376	its molecular structure	354:376	its molecular structure	354:376	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	4	22	theme	nuclear	543:549	arg1	polarization					551:562	dynamic nuclear polarization	535:562	dynamic nuclear polarization	535:562	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	23	theme	high	77:80	arg1	mortality					82:90	The high mortality	73:90	The high mortality of invasive fungal infections	73:120	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	24	theme	antifungals	164:174	arg1	mortality					82:90	The high mortality	73:90	The high mortality of invasive fungal infections	73:120	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	24	theme	antifungals	164:174	arg1	inefficacy					150:159	inefficacy	150:159	inefficacy	150:159	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	24	theme	antifungals	164:174	arg1	number					139:144	limited number	131:144	limited number	131:144	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	0	25	theme	Molecular	0:8	arg1	architecture					10:21	Molecular architecture	0:21	Molecular architecture of fungal cell walls	0:42	Molecular architecture of fungal cell walls revealed by solid-state NMR.					
30013106	4	26	theme	dynamic	535:541	arg1	polarization					551:562	dynamic nuclear polarization	535:562	dynamic nuclear polarization	535:562	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	4	27	theme	β-glucans	721:729	arg1	matrix					694:699	a hydrated matrix	683:699	a hydrated matrix of diversely linked β-glucans	683:729	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	4	28	theme	dynamic	747:753	arg1	layer					755:759	a dynamic layer	745:759	a dynamic layer of glycoproteins and α-1,3-glucan	745:793	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	5	29	theme	molecule	878:885	arg1	functions					860:868	the dual functions	851:868	the dual functions of this molecule:	851:886	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	0	30	theme	fungal	26:31	arg1	walls					38:42	fungal cell walls	26:42	fungal cell walls	26:42	Molecular architecture of fungal cell walls revealed by solid-state NMR.					
30013106	5	31	theme	two-domain	800:809	arg1	distribution					811:822	The two-domain distribution	796:822	The two-domain distribution of α-1,3-glucans	796:839	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	5	32	theme	fungal	927:932	arg1	virulence					934:942	fungal virulence	927:942	fungal virulence	927:942	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	2	33	theme	promising	279:287	arg1	wall					269:272	The fungal cell wall	253:272	The fungal cell wall	253:272	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	2	33	theme	promising	279:287	arg1	target					289:294	a promising target	277:294	a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive	277:392	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	3	34	theme	cell	434:437	arg1	walls					439:443	the cell walls	430:443	the cell walls	430:443	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	5	35	dep	wall	909:912	arg1	rigidity					914:921	rigidity	914:921	rigidity	914:921	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	1	36	theme	invasive	95:102	arg1	infections					111:120	invasive fungal infections	95:120	invasive fungal infections	95:120	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	3	37	theme	walls	439:443	arg1	architecture					414:425	the architecture	410:425	the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus	410:490	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	0	38	theme	walls	38:42	arg1	architecture					10:21	Molecular architecture	0:21	Molecular architecture of fungal cell walls	0:42	Molecular architecture of fungal cell walls revealed by solid-state NMR.					
30013106	3	39	dep	Aspergillus	470:480	arg1	fumigatus					482:490	fumigatus	482:490	fumigatus	482:490	Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus.					
30013106	0	40	theme	cell	33:36	arg1	walls					38:42	fungal cell walls	26:42	fungal cell walls	26:42	Molecular architecture of fungal cell walls revealed by solid-state NMR.					
30013106	1	41	theme	new	207:209	arg1	agents					211:216	new agents	207:216	new agents with novel mechanisms and targets	207:250	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	4	42	theme	NMR	505:507	arg1	spectroscopy					509:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	6	43	theme	high-resolution	967:981	arg1	model					983:987	a high-resolution model	965:987	a high-resolution model of fungal cell walls	965:1008	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	2	44	theme	absent	327:332	arg1	polysaccharides					311:325	polysaccharides	311:325	polysaccharides absent in humans	311:342	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	4	45	theme	assisted	523:530	arg1	spectroscopy					509:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy	493:520	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	46	theme	infections	111:120	arg1	mortality					82:90	The high mortality	73:90	The high mortality of invasive fungal infections	73:120	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	46	theme	infections	111:120	arg1	inefficacy					150:159	inefficacy	150:159	inefficacy	150:159	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	46	theme	infections	111:120	arg1	number					139:144	limited number	131:144	limited number	131:144	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	6	47	theme	walls	1004:1008	arg1	model					983:987	a high-resolution model	965:987	a high-resolution model of fungal cell walls	965:1008	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	4	48	theme	linked	714:719	arg1	β-glucans					721:729	diversely linked β-glucans	704:729	diversely linked β-glucans	704:729	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	49	theme	agents	211:216	arg1	development					192:202	the development	188:202	the development of new agents with novel mechanisms and targets	188:250	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	4	50	theme	glycoproteins	764:776	arg1	layer					755:759	a dynamic layer	745:759	a dynamic layer of glycoproteins and α-1,3-glucan	745:793	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	2	51	dep	target	289:294	arg1	contains					302:309	contains	302:309	contains polysaccharides absent in humans	302:342	The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive.					
30013106	5	52	theme	dual	855:858	arg1	functions					860:868	the dual functions	851:868	the dual functions of this molecule:	851:886	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	5	53	theme	α-1,3-glucans	827:839	arg1	distribution					811:822	The two-domain distribution	796:822	The two-domain distribution of α-1,3-glucans	796:839	The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence.					
30013106	6	54	theme	antifungals	1106:1116	arg1	development					1077:1087	the development	1073:1087	the development of wall-targeted antifungals	1073:1116	This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.					
30013106	4	55	theme	hydrophobic	640:650	arg1	scaffold					652:659	a hydrophobic scaffold	638:659	a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan	638:793	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	56	theme	novel	223:227	arg1	mechanisms					229:238	novel mechanisms	223:238	novel mechanisms	223:238	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	0	57	theme	solid-state	56:66	arg1	NMR					68:70	solid-state NMR	56:70	solid-state NMR	56:70	Molecular architecture of fungal cell walls revealed by solid-state NMR.					
30013106	4	58	theme	α-1,3-glucan	782:793	arg1	layer					755:759	a dynamic layer	745:759	a dynamic layer of glycoproteins and α-1,3-glucan	745:793	Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan.					
30013106	1	59	with	agents	211:216	arg1	targets					244:250	targets	244:250	targets	244:250	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	59	with	agents	211:216	arg1	mechanisms					229:238	novel mechanisms	223:238	novel mechanisms	223:238	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
30013106	1	60	theme	limited	131:137	arg1	number					139:144	limited number	131:144	limited number	131:144	The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets.					
31888812	0	0	from	structure	15:23	arg1	Anoectochilus					83:95	Anoectochilus	83:95	Anoectochilus	83:95	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	2	1	theme	weight	368:373	arg1	weights					393:399	the relative weight average molecular weights	355:399	the relative weight average molecular weights	355:399	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	6	2	theme	conformational	1002:1015	arg1	properties					1017:1026	structural and conformational properties	987:1026	structural and conformational properties of ARPP-40	987:1037	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	2	3	theme	relative	359:366	arg1	weights					393:399	the relative weight average molecular weights	355:399	the relative weight average molecular weights	355:399	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	3	4	theme	main	680:683	arg1	components					685:694	the main components	676:694	the main components	676:694	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	4	theme	main	680:683	arg1	galactose					661:669	galactose	661:669	galactose	661:669	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	4	theme	main	680:683	arg1	glucose					649:655	glucose	649:655	glucose	649:655	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	6	5	from	application	1070:1080	arg1	food					1085:1088	food	1085:1088	food	1085:1088	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	6	5	from	application	1070:1080	arg1	industry					1104:1111	medicinal industry	1094:1111	medicinal industry	1094:1111	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	3	6	theme	monosaccharide	527:540	arg1	composition					542:552	monosaccharide composition	527:552	monosaccharide composition	527:552	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	2	7	dep	showed	348:353	arg1	10.8 kDa					480:487	10.8 kDa	480:487	10.8 kDa	480:487	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	2	7	dep	showed	348:353	arg1	%					498:498	51.4 %	493:498	51.4 %	493:498	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	2	7	dep	showed	348:353	arg1	%					477:477	423 kDa and 97.4 %	460:477	%	477:477	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	6	8	theme	structural	987:996	arg1	properties					1017:1026	structural and conformational properties	987:1026	structural and conformational properties of ARPP-40	987:1037	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	4	9	theme	ARPP-40	760:766	arg1	conformation					728:739	conformation	728:739	conformation	728:739	Furthermore, the structure and conformation characteristics of ARPP-40 were systematically investigated.					
31888812	4	9	theme	ARPP-40	760:766	arg1	structure					714:722	structure	714:722	structure	714:722	Furthermore, the structure and conformation characteristics of ARPP-40 were systematically investigated.					
31888812	3	10	theme	composition	542:552	arg1	terms					518:522	terms	518:522	terms of monosaccharide composition	518:552	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	5	11	with	chain	890:894	arg1	index					918:922	a polydispersity index	901:922	a polydispersity index of 1.02 in 0.1 M NaNO3 solution	901:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	5	12	theme	1.02	927:930	arg1	index					918:922	a polydispersity index	901:922	a polydispersity index of 1.02 in 0.1 M NaNO3 solution	901:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	1	13	dep	ARPP-40	143:149	arg1	%					155:155	40 %	152:155	40 % ethanol precipitate	152:175	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	6	14	theme	medicinal	1094:1102	arg1	industry					1104:1111	medicinal industry	1094:1111	medicinal industry	1094:1111	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	2	15	theme	423 kDa	460:466	arg1	%					477:477	423 kDa and 97.4 %	460:477	%	477:477	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	0	16	from	conformation	29:40	arg1	Anoectochilus					83:95	Anoectochilus	83:95	Anoectochilus	83:95	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	5	17	from	1.02	927:930	arg1	solution					947:954	0.1 M NaNO3 solution	935:954	0.1 M NaNO3 solution	935:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	4	18	dep	structure	714:722	arg1	characteristics					741:755	characteristics	741:755	characteristics	741:755	Furthermore, the structure and conformation characteristics of ARPP-40 were systematically investigated.					
31888812	4	18	dep	structure	714:722	arg1	the					710:712	the	710:712	the	710:712	Furthermore, the structure and conformation characteristics of ARPP-40 were systematically investigated.					
31888812	1	19	theme	new	113:115	arg1	ARPP-70					182:188	ARPP-70	182:188	ARPP-70	182:188	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	1	19	theme	new	113:115	arg1	ARPP-40					143:149	ARPP-40	143:149	ARPP-40	143:149	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	1	19	theme	new	113:115	arg1	polysaccharides					126:140	isolated polysaccharides	117:140	Two new isolated polysaccharides	109:140	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	6	20	theme	systematic	961:970	arg1	meaningful					1043:1052	meaningful	1043:1052	meaningful	1043:1052	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	6	20	theme	systematic	961:970	arg1	information					972:982	The systematic information	957:982	The systematic information on structural and conformational properties of ARPP-40	957:1037	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	5	21	from	index	918:922	arg1	solution					947:954	0.1 M NaNO3 solution	935:954	0.1 M NaNO3 solution	935:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	1	22	dep	%	155:155	arg1	precipitate					165:175	ethanol precipitate	157:175	40 % ethanol precipitate	152:175	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	6	23	theme	further	1062:1068	arg1	application					1070:1080	its further application	1058:1080	its further application in food and medicinal industry	1058:1111	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	5	24	theme	0.1 M	935:939	arg1	solution					947:954	0.1 M NaNO3 solution	935:954	0.1 M NaNO3 solution	935:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	2	25	theme	polysaccharides	302:316	arg1	properties					284:293	The physicochemical properties	264:293	The physicochemical properties of two polysaccharides	264:316	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	1	26	theme	ethanol	196:202	arg1	precipitate					204:214	ethanol precipitate	196:214	70 % ethanol precipitate	191:214	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	3	27	contain	contained	563:571	arg1	ARPP-40					555:561	ARPP-40	555:561	ARPP-40	555:561	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	27	contain	contained	563:571	arg1	terms					518:522	terms	518:522	terms of monosaccharide composition	518:552	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	27	contain	contained	563:571	arg2	ARPP-40					555:561	ARPP-40	555:561	ARPP-40	555:561	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	27	contain	contained	563:571	arg2	glucose					578:584	glucose	578:584	glucose	578:584	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	6	28	from	information	972:982	arg1	properties					1017:1026	structural and conformational properties	987:1026	structural and conformational properties of ARPP-40	987:1037	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	2	29	theme	sugar	425:429	arg1	contents					405:412	contents	405:412	contents	405:412	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	2	29	theme	sugar	425:429	arg1	weights					393:399	the relative weight average molecular weights	355:399	the relative weight average molecular weights	355:399	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	0	30	from	Anoectochilus	83:95	arg1	structure					15:23	structure	15:23	structure	15:23	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	0	30	from	Anoectochilus	83:95	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	0	30	from	Anoectochilus	83:95	arg1	conformation					29:40	conformation	29:40	conformation	29:40	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	0	30	from	Anoectochilus	83:95	arg1	polysaccharides					62:76	polysaccharides	62:76	polysaccharides from Anoectochilus roxburghii	62:106	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	2	31	theme	neutral	417:423	arg1	sugar					425:429	neutral sugar	417:429	neutral sugar	417:429	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	2	32	theme	97.4	472:475	arg1	%					477:477	423 kDa and 97.4 %	460:477	%	477:477	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	5	33	theme	flexible	881:888	arg1	chain					890:894	a flexible chain	879:894	a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution	879:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	5	34	theme	polydispersity	903:916	arg1	index					918:922	a polydispersity index	901:922	a polydispersity index of 1.02 in 0.1 M NaNO3 solution	901:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	5	35	theme	NaNO3	941:945	arg1	solution					947:954	0.1 M NaNO3 solution	935:954	0.1 M NaNO3 solution	935:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	1	36	dep	ARPP-70	182:188	arg1	%					194:194	70 %	191:194	70 % ethanol precipitate	191:214	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	3	37	theme	monosaccharides	623:637	arg1	components					685:694	the main components	676:694	the main components	676:694	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	37	theme	monosaccharides	623:637	arg1	galactose					661:669	galactose	661:669	galactose	661:669	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	3	37	theme	monosaccharides	623:637	arg1	glucose					649:655	glucose	649:655	glucose	649:655	In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components.					
31888812	1	38	theme	isolated	117:124	arg1	ARPP-70					182:188	ARPP-70	182:188	ARPP-70	182:188	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	1	38	theme	isolated	117:124	arg1	ARPP-40					143:149	ARPP-40	143:149	ARPP-40	143:149	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	1	38	theme	isolated	117:124	arg1	polysaccharides					126:140	isolated polysaccharides	117:140	Two new isolated polysaccharides	109:140	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	2	39	theme	physicochemical	268:282	arg1	properties					284:293	The physicochemical properties	264:293	The physicochemical properties of two polysaccharides	264:316	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	0	40	dep	structure	15:23	arg1	characterization					42:57	characterization	42:57	characterization	42:57	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	1	41	theme	ethanol	157:163	arg1	precipitate					165:175	ethanol precipitate	157:175	40 % ethanol precipitate	152:175	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	6	42	theme	ARPP-40	1031:1037	arg1	properties					1017:1026	structural and conformational properties	987:1026	structural and conformational properties of ARPP-40	987:1037	The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.					
31888812	1	43	dep	%	194:194	arg1	precipitate					204:214	ethanol precipitate	196:214	70 % ethanol precipitate	191:214	Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii.					
31888812	2	44	theme	molecular	383:391	arg1	weights					393:399	the relative weight average molecular weights	355:399	the relative weight average molecular weights	355:399	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
31888812	5	45	from	solution	947:954	arg1	index					918:922	a polydispersity index	901:922	a polydispersity index of 1.02 in 0.1 M NaNO3 solution	901:954	The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution.					
31888812	0	46	theme	polysaccharides	62:76	arg1	structure					15:23	structure	15:23	structure	15:23	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	0	46	theme	polysaccharides	62:76	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	0	46	theme	polysaccharides	62:76	arg1	conformation					29:40	conformation	29:40	conformation	29:40	Fractionation, structure and conformation characterization of polysaccharides from Anoectochilus roxburghii.					
31888812	2	47	theme	average	375:381	arg1	weights					393:399	the relative weight average molecular weights	355:399	the relative weight average molecular weights	355:399	The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively.					
28927239	6	0	theme	substrate	1068:1076	arg1	acid					1093:1096	GADC substrate UDP-glucuronic acid	1063:1096	GADC substrate UDP-glucuronic acid	1063:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	2	1	theme	involvement	302:312	arg1	scope					287:291	the scope	283:291	the scope of their involvement during the orchestration of this resource-demanding process	283:372	While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process.					
28927239	10	2	theme	cell	1745:1748	arg1	changes					1769:1775	underlying cell wall compositional changes	1734:1775	underlying cell wall compositional changes that were caused by tubulin manipulation	1734:1816	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	4	3	theme	poplar	628:633	arg1	lines					635:639	transgenic poplar lines	617:639	transgenic poplar lines with constitutive overexpression of α-tubulin (TUA)	617:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	10	4	theme	stomatal	1536:1543	arg1	opening					1545:1551	stomatal opening	1536:1551	stomatal opening	1536:1551	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	2	5	theme	cell	234:237	arg1	biogenesis					244:253	cell wall biogenesis	234:253	cell wall biogenesis	234:253	While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process.					
28927239	9	6	theme	increased	1417:1425	arg1	levels					1427:1432	increased levels	1417:1432	increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol	1417:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	5	7	theme	decarboxylase	852:864	arg1	expression					818:827	altered expression	810:827	altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves	810:896	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	6	8	theme	altered	930:936	arg1	control					946:952	altered diurnal control	930:952	altered diurnal control of TUA through its constitutive overexpression	930:999	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	4	9	theme	cell	603:606	arg1	walls					608:612	Leaf cell walls	598:612	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA)	598:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	6	10	theme	GADC	1063:1066	arg1	acid					1093:1096	GADC substrate UDP-glucuronic acid	1063:1096	GADC substrate UDP-glucuronic acid	1063:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	9	11	theme	substrate	1441:1449	arg1	homogalacturonan					1451:1466	PME substrate homogalacturonan	1437:1466	PME substrate homogalacturonan	1437:1466	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	1	12	theme	Plant	75:79	arg1	walls					86:90	Plant cell walls	75:90	Plant cell walls	75:90	Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues.					
28927239	3	13	theme	leaf	571:574	arg1	biogenesis					586:595	poplar leaf cell wall biogenesis	564:595	poplar leaf cell wall biogenesis	564:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	7	14	from	effects	1183:1189	arg1	expression					1240:1249	associated gene expression	1224:1249	associated gene expression	1224:1249	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	7	14	from	effects	1183:1189	arg1	composition					1208:1218	hemicellulose composition	1194:1218	hemicellulose composition	1194:1218	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	10	15	theme	microtubule	1601:1611	arg1	dynamics					1613:1620	fundamentally contrasting microtubule dynamics	1575:1620	fundamentally contrasting microtubule dynamics	1575:1620	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	10	16	theme	underlying	1734:1743	arg1	changes					1769:1775	underlying cell wall compositional changes	1734:1775	underlying cell wall compositional changes that were caused by tubulin manipulation	1734:1816	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	9	17	theme	stomatal	1474:1481	arg1	kinetics					1483:1490	slow stomatal kinetics	1469:1490	slow stomatal kinetics	1469:1490	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	8	18	theme	enzymatic	1291:1299	arg1	activity					1301:1308	enzymatic activity	1291:1308	enzymatic activity	1291:1308	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	1	19	theme	cell	81:84	arg1	walls					86:90	Plant cell walls	75:90	Plant cell walls	75:90	Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues.					
28927239	0	20	from	all	56:58	arg1	timing					67:72	the timing	63:72	the timing	63:72	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	3	21	theme	wall	581:584	arg1	biogenesis					586:595	poplar leaf cell wall biogenesis	564:595	poplar leaf cell wall biogenesis	564:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	4	22	from	reduction	760:768	arg1	xylose					773:778	xylose	773:778	xylose	773:778	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	9	23	theme	fate	1500:1503	arg1	levels					1427:1432	increased levels	1417:1432	increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol	1417:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	0	24	from	rhythms	10:16	arg1	poplar					36:41	poplar	36:41	poplar	36:41	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	4	25	theme	α-tubulin	677:685	arg1	overexpression					659:672	constitutive overexpression	646:672	constitutive overexpression of α-tubulin (TUA)	646:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	7	26	theme	hemicellulose	1194:1206	arg1	composition					1208:1218	hemicellulose composition	1194:1218	hemicellulose composition	1194:1218	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	1	27	theme	compositional	118:130	arg1	changes					132:138	architectural and compositional changes	100:138	architectural and compositional changes	100:138	Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues.					
28927239	6	28	theme	TUA	957:959	arg1	control					946:952	altered diurnal control	930:952	altered diurnal control of TUA through its constitutive overexpression	930:999	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	0	29	from	Tubulins	0:7	arg1	poplar					36:41	poplar	36:41	poplar	36:41	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	9	30	theme	methanol	1520:1527	arg1	kinetics					1483:1490	slow stomatal kinetics	1469:1490	slow stomatal kinetics	1469:1490	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	9	30	theme	methanol	1520:1527	arg1	fate					1500:1503	the fate	1496:1503	the fate of PME product methanol	1496:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	9	30	theme	methanol	1520:1527	arg1	homogalacturonan					1451:1466	PME substrate homogalacturonan	1437:1466	PME substrate homogalacturonan	1437:1466	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	2	31	theme	process	366:372	arg1	orchestration					325:337	the orchestration	321:337	the orchestration of this resource-demanding process	321:372	While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process.					
28927239	4	32	theme	increased	706:714	arg1	abundance					716:724	an increased abundance	703:724	an increased abundance of homogalacturonan	703:744	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	7	33	theme	lignin	1158:1163	arg1	abundance					1165:1173	lignin abundance	1158:1173	lignin abundance	1158:1173	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	3	34	theme	data	509:512	arg1	wall					415:418	cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data	410:512	wall	415:418	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	3	34	theme	data	509:512	arg1	mining					456:461	mining	456:461	mining of publicly available diurnal gene expression data	456:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	3	34	theme	data	509:512	arg1	RNA-seq					444:450	RNA-seq	444:450	RNA-seq	444:450	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	6	35	theme	metabolic	1010:1018	arg1	tradeoff					1020:1027	a metabolic tradeoff	1008:1027	a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid	1008:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	3	36	theme	transgenic	377:386	arg1	approach					388:395	A transgenic approach	375:395	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data	375:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	4	37	with	lines	635:639	arg1	overexpression					659:672	constitutive overexpression	646:672	constitutive overexpression of α-tubulin (TUA)	646:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	3	38	theme	compositional	420:432	arg1	analysis					434:441	compositional analysis	420:441	compositional analysis	420:441	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	0	39	theme	cell	22:25	arg1	walls					27:31	cell walls	22:31	cell walls	22:31	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	3	40	dep	wall	415:418	arg1	analysis					434:441	compositional analysis	420:441	compositional analysis	420:441	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	8	41	dep	transgenic	1358:1367	arg1	leaves					1369:1374	leaves	1369:1374	leaves	1369:1374	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	1	42	theme	external	188:195	arg1	cues					197:200	external cues	188:200	external cues	188:200	Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues.					
28927239	1	43	theme	architectural	100:112	arg1	changes					132:138	architectural and compositional changes	100:138	architectural and compositional changes	100:138	Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues.					
28927239	3	44	theme	gene	493:496	arg1	data					509:512	publicly available diurnal gene expression data	466:512	publicly available diurnal gene expression data	466:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	5	45	theme	UDP-glucuronic	832:845	arg1	GADC					867:870	GADC	867:870	GADC	867:870	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	5	45	theme	UDP-glucuronic	832:845	arg1	decarboxylase					852:864	UDP-glucuronic acid decarboxylase	832:864	UDP-glucuronic acid decarboxylase (GADC)	832:871	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	0	46	dep	Tubulins	0:7	arg1	all					56:58	it's all	51:58	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.	0:73	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	6	47	theme	UDP-glucuronic	1078:1091	arg1	acid					1093:1096	GADC substrate UDP-glucuronic acid	1063:1096	GADC substrate UDP-glucuronic acid	1063:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	3	48	theme	available	475:483	arg1	data					509:512	publicly available diurnal gene expression data	466:512	publicly available diurnal gene expression data	466:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	7	49	from	effects	1119:1125	arg1	hemicellulose					1141:1153	hemicellulose	1141:1153	hemicellulose	1141:1153	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	7	49	from	effects	1119:1125	arg1	abundance					1165:1173	lignin abundance	1158:1173	lignin abundance	1158:1173	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	7	49	from	effects	1119:1125	arg1	cellulose					1130:1138	cellulose	1130:1138	cellulose	1130:1138	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	7	50	theme	gene	1235:1238	arg1	expression					1240:1249	associated gene expression	1224:1249	associated gene expression	1224:1249	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	10	51	theme	wall	1750:1753	arg1	changes					1769:1775	underlying cell wall compositional changes	1734:1775	underlying cell wall compositional changes that were caused by tubulin manipulation	1734:1816	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	8	52	theme	methylesterase	1320:1333	arg1	expression					1276:1285	expression	1276:1285	expression	1276:1285	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	8	52	theme	methylesterase	1320:1333	arg1	activity					1301:1308	enzymatic activity	1291:1308	enzymatic activity	1291:1308	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	2	53	theme	wall	239:242	arg1	biogenesis					244:253	cell wall biogenesis	234:253	cell wall biogenesis	234:253	While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process.					
28927239	9	54	theme	levels	1427:1432	arg1	context					1406:1412	a context	1404:1412	a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol	1404:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	4	55	theme	transgenic	617:626	arg1	lines					635:639	transgenic poplar lines	617:639	transgenic poplar lines with constitutive overexpression of α-tubulin (TUA)	617:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	10	56	theme	contrasting	1589:1599	arg1	dynamics					1613:1620	fundamentally contrasting microtubule dynamics	1575:1620	fundamentally contrasting microtubule dynamics	1575:1620	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	5	57	theme	acid	847:850	arg1	GADC					867:870	GADC	867:870	GADC	867:870	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	5	57	theme	acid	847:850	arg1	decarboxylase					852:864	UDP-glucuronic acid decarboxylase	832:864	UDP-glucuronic acid decarboxylase (GADC)	832:871	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	6	58	theme	diurnal	938:944	arg1	control					946:952	altered diurnal control	930:952	altered diurnal control of TUA through its constitutive overexpression	930:999	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	3	59	used	used	518:521	arg2	approach					388:395	A transgenic approach	375:395	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data	375:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	9	60	theme	PME	1437:1439	arg1	homogalacturonan					1451:1466	PME substrate homogalacturonan	1437:1466	PME substrate homogalacturonan	1437:1466	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	9	61	theme	PME	1508:1510	arg1	methanol					1520:1527	PME product methanol	1508:1527	PME product methanol	1508:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	4	62	theme	Leaf	598:601	arg1	walls					608:612	Leaf cell walls	598:612	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA)	598:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	3	63	theme	poplar	564:569	arg1	biogenesis					586:595	poplar leaf cell wall biogenesis	564:595	poplar leaf cell wall biogenesis	564:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	4	64	theme	lines	635:639	arg1	walls					608:612	Leaf cell walls	598:612	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA)	598:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	5	65	theme	altered	810:816	arg1	expression					818:827	altered expression	810:827	altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves	810:896	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	3	66	theme	tubulins	552:559	arg1	involvement					537:547	the involvement	533:547	the involvement of tubulins in poplar leaf cell wall biogenesis	533:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	5	67	from	expression	818:827	arg1	transgenic					880:889	transgenic	880:889	transgenic	880:889	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	9	68	theme	slow	1469:1472	arg1	kinetics					1483:1490	slow stomatal kinetics	1469:1490	slow stomatal kinetics	1469:1490	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	9	69	theme	kinetics	1483:1490	arg1	levels					1427:1432	increased levels	1417:1432	increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol	1417:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	3	70	from	involvement	537:547	arg1	biogenesis					586:595	poplar leaf cell wall biogenesis	564:595	poplar leaf cell wall biogenesis	564:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	3	71	theme	cell	576:579	arg1	biogenesis					586:595	poplar leaf cell wall biogenesis	564:595	poplar leaf cell wall biogenesis	564:595	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	6	72	theme	constitutive	973:984	arg1	overexpression					986:999	its constitutive overexpression	969:999	its constitutive overexpression	969:999	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	4	73	theme	constitutive	646:657	arg1	overexpression					659:672	constitutive overexpression	646:672	constitutive overexpression of α-tubulin (TUA)	646:691	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	10	74	theme	processes	1643:1651	arg1	slowing					1627:1633	the slowing	1623:1633	the slowing of both processes in the transgenic lines as previously reported	1623:1698	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	10	74	theme	processes	1643:1651	arg1	related					1723:1729	related	1723:1729	related	1723:1729	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	8	75	theme	pectin	1313:1318	arg1	PME					1336:1338	PME	1336:1338	PME	1336:1338	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	8	75	theme	pectin	1313:1318	arg1	methylesterase					1320:1333	pectin methylesterase	1313:1333	pectin methylesterase (PME)	1313:1339	In addition, expression and enzymatic activity of pectin methylesterase (PME) decreased in the transgenic leaves.					
28927239	7	76	theme	subtle	1176:1181	arg1	effects					1183:1189	subtle effects	1176:1189	subtle effects on hemicellulose composition and associated gene expression	1176:1249	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
28927239	9	77	theme	product	1512:1518	arg1	methanol					1520:1527	PME product methanol	1508:1527	PME product methanol	1508:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	10	78	theme	compositional	1755:1767	arg1	changes					1769:1775	underlying cell wall compositional changes	1734:1775	underlying cell wall compositional changes that were caused by tubulin manipulation	1734:1816	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	4	79	theme	homogalacturonan	729:744	arg1	abundance					716:724	an increased abundance	703:724	an increased abundance of homogalacturonan	703:744	Leaf cell walls of transgenic poplar lines with constitutive overexpression of α-tubulin (TUA) exhibited an increased abundance of homogalacturonan, along with a reduction in xylose.					
28927239	0	80	from	walls	27:31	arg1	poplar					36:41	poplar	36:41	poplar	36:41	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	5	81	dep	transgenic	880:889	arg1	leaves					891:896	leaves	891:896	leaves	891:896	These changes were traced to altered expression of UDP-glucuronic acid decarboxylase (GADC) in the transgenic leaves.					
28927239	2	82	theme	resource-demanding	347:364	arg1	process					366:372	this resource-demanding process	342:372	this resource-demanding process	342:372	While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process.					
28927239	6	83	theme	cellular	1039:1046	arg1	utilization					1048:1058	cellular utilization	1039:1058	cellular utilization of GADC substrate UDP-glucuronic acid	1039:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	10	84	theme	transgenic	1660:1669	arg1	lines					1671:1675	the transgenic lines	1656:1675	the transgenic lines	1656:1675	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	9	85	theme	homogalacturonan	1451:1466	arg1	levels					1427:1432	increased levels	1417:1432	increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol	1417:1527	The change is discussed in a context of increased levels of PME substrate homogalacturonan, slow stomatal kinetics and the fate of PME product methanol.					
28927239	0	86	dep	poplar	36:41	arg1	leaves					43:48	leaves	43:48	leaves	43:48	Tubulins, rhythms and cell walls in poplar leaves: it's all in the timing.					
28927239	3	87	theme	cell	410:413	arg1	wall					415:418	cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data	410:512	wall	415:418	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	10	88	from	slowing	1627:1633	arg1	lines					1671:1675	the transgenic lines	1656:1675	the transgenic lines	1656:1675	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	3	89	theme	diurnal	485:491	arg1	data					509:512	publicly available diurnal gene expression data	466:512	publicly available diurnal gene expression data	466:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	10	90	theme	tubulin	1797:1803	arg1	manipulation					1805:1816	tubulin manipulation	1797:1816	tubulin manipulation	1797:1816	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	10	91	dep	slowing	1627:1633	arg1	reported					1691:1698	reported	1691:1698	reported	1691:1698	Since stomatal opening and closing depend on fundamentally contrasting microtubule dynamics, the slowing of both processes in the transgenic lines as previously reported appears to be directly related to underlying cell wall compositional changes that were caused by tubulin manipulation.					
28927239	6	92	theme	acid	1093:1096	arg1	utilization					1048:1058	cellular utilization	1039:1058	cellular utilization of GADC substrate UDP-glucuronic acid	1039:1096	A model is postulated by which altered diurnal control of TUA through its constitutive overexpression led to a metabolic tradeoff affecting cellular utilization of GADC substrate UDP-glucuronic acid.					
28927239	3	93	theme	expression	498:507	arg1	data					509:512	publicly available diurnal gene expression data	466:512	publicly available diurnal gene expression data	466:512	A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis.					
28927239	7	94	theme	associated	1224:1233	arg1	expression					1240:1249	associated gene expression	1224:1249	associated gene expression	1224:1249	While there were no effects on cellulose, hemicellulose or lignin abundance, subtle effects on hemicellulose composition and associated gene expression were noted.					
31602875	1	0	from	compounds	175:183	arg1	catenatum					199:207	Dendrobium catenatum	188:207	Dendrobium catenatum	188:207	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	3	1	theme	water	513:517	arg1	extraction					519:528	" water extraction	511:528	" water extraction	511:528	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	12	2	theme	polysaccharides	1271:1285	arg1	determination					1243:1255	the determination	1239:1255	the determination of non-starch polysaccharides in other starch-containing medicinal materials	1239:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	7	3	dep	58	875:876	arg1	%					873:873	9%	872:873	9%	872:873	9%,58.					
31602875	1	4	theme	major	159:163	arg1	polysaccharides					103:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	1	4	theme	major	159:163	arg1	compounds					175:183	the major bioactive compounds	155:183	the major bioactive compounds in Dendrobium catenatum	155:207	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	10	5	theme	non-starch	958:967	arg1	polysaccharides					969:983	retained non-starch polysaccharides	949:983	retained non-starch polysaccharides	949:983	The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable.					
31602875	6	6	theme	polysaccharides	814:828	arg1	contents					796:803	the contents	792:803	the contents of total polysaccharides,glucose,and mannose	792:848	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	11	7	theme	D.	1124:1125	arg1	polysaccharides					1105:1119	non-starch polysaccharides	1094:1119	non-starch polysaccharides of D. catenatum	1094:1135	Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum.					
31602875	6	8	theme	total	808:812	arg1	glucose					830:836	glucose	830:836	glucose	830:836	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	6	8	theme	total	808:812	arg1	mannose					842:848	mannose	842:848	mannose	842:848	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	6	8	theme	total	808:812	arg1	polysaccharides					814:828	total polysaccharides	808:828	total polysaccharides	808:828	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	3	9	theme	alcohol	557:563	arg1	precipitation					565:577	alcohol precipitation	557:577	alcohol precipitation	557:577	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	12	10	theme	D.	1207:1208	arg1	control					1196:1202	quality control	1188:1202	quality control of D. catenatum	1188:1218	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	0	11	from	polysaccharides	46:60	arg1	catenatum					76:84	Dendrobium catenatum	65:84	Dendrobium catenatum	65:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	12	12	theme	starch-containing	1296:1312	arg1	materials					1324:1332	other starch-containing medicinal materials	1290:1332	other starch-containing medicinal materials	1290:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	1	13	theme	bioactive	165:173	arg1	polysaccharides					103:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	1	13	theme	bioactive	165:173	arg1	compounds					175:183	the major bioactive compounds	155:183	the major bioactive compounds in Dendrobium catenatum	155:207	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	2	14	theme	D.	392:393	arg1	strain					409:414	D. catenatum C15 strain	392:414	D. catenatum C15 strain collected from the pear epiphytic cultivation	392:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	12	15	dep	D.	1207:1208	arg1	catenatum					1210:1218	D. catenatum	1207:1218	D. catenatum	1207:1218	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	12	16	from	reference	1226:1234	arg1	determination					1243:1255	the determination	1239:1255	the determination of non-starch polysaccharides in other starch-containing medicinal materials	1239:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	3	17	theme	"	583:583	arg1	method					585:590	" method	583:590	" method	583:590	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	12	18	theme	non-starch	1260:1269	arg1	polysaccharides					1271:1285	non-starch polysaccharides	1260:1285	non-starch polysaccharides	1260:1285	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	2	19	theme	epiphytic	440:448	arg1	cultivation					450:460	the pear epiphytic cultivation	431:460	the pear epiphytic cultivation	431:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	1	20	theme	non-starch	92:101	arg1	polysaccharides					103:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides	88:117	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	1	20	theme	non-starch	92:101	arg1	compounds					175:183	the major bioactive compounds	155:183	the major bioactive compounds in Dendrobium catenatum	155:207	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	1	21	theme	Dendrobium	188:197	arg1	catenatum					199:207	Dendrobium catenatum	188:207	Dendrobium catenatum	188:207	The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum.					
31602875	2	22	theme	pear	435:438	arg1	cultivation					450:460	the pear epiphytic cultivation	431:460	the pear epiphytic cultivation	431:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	11	23	theme	non-starch	1094:1103	arg1	polysaccharides					1105:1119	non-starch polysaccharides	1094:1119	non-starch polysaccharides of D. catenatum	1094:1135	Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum.					
31602875	12	24	theme	other	1290:1294	arg1	materials					1324:1332	other starch-containing medicinal materials	1290:1332	other starch-containing medicinal materials	1290:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	3	25	theme	non-starch	467:476	arg1	polysaccharides					478:492	The non-starch polysaccharides	463:492	The non-starch polysaccharides	463:492	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	0	26	theme	quantification	10:23	arg1	method					25:30	quantification method	10:30	quantification method of non-starch polysaccharides in Dendrobium catenatum	10:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	10	27	theme	analysis	993:1000	arg1	accurate					1013:1020	accurate	1013:1020	accurate	1013:1020	The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable.					
31602875	10	27	theme	analysis	993:1000	arg1	result					1002:1007	the analysis result	989:1007	the analysis result	989:1007	The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable.					
31602875	2	28	theme	polysaccharides	348:362	arg1	method					327:332	a quantification method	310:332	a quantification method of non-starch polysaccharides	310:362	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	0	29	theme	non-starch	35:44	arg1	polysaccharides					46:60	non-starch polysaccharides	35:60	non-starch polysaccharides in Dendrobium catenatum	35:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	2	30	theme	strain	409:414	arg1	stems					383:387	stems	383:387	stems of D. catenatum C15 strain collected from the pear epiphytic cultivation	383:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	2	31	theme	catenatum	395:403	arg1	strain					409:414	D. catenatum C15 strain	392:414	D. catenatum C15 strain collected from the pear epiphytic cultivation	392:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	12	32	from	determination	1243:1255	arg1	materials					1324:1332	other starch-containing medicinal materials	1290:1332	other starch-containing medicinal materials	1290:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	0	33	from	catenatum	76:84	arg1	method					25:30	quantification method	10:30	quantification method of non-starch polysaccharides in Dendrobium catenatum	10:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	12	34	theme	medicinal	1314:1322	arg1	materials					1324:1332	other starch-containing medicinal materials	1290:1332	other starch-containing medicinal materials	1290:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	2	35	theme	C15	405:407	arg1	strain					409:414	D. catenatum C15 strain	392:414	D. catenatum C15 strain collected from the pear epiphytic cultivation	392:460	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	2	36	theme	non-starch	337:346	arg1	polysaccharides					348:362	non-starch polysaccharides	337:362	non-starch polysaccharides	337:362	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	0	37	theme	polysaccharides	46:60	arg1	method					25:30	quantification method	10:30	quantification method of non-starch polysaccharides in Dendrobium catenatum	10:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	5	38	theme	system	696:701	arg1	suitability					703:713	the system suitability	692:713	the system suitability	692:713	In addition,the system suitability was tested.					
31602875	11	39	theme	content	1071:1077	arg1	measurement					1079:1089	the content measurement	1067:1089	the content measurement of non-starch polysaccharides of D. catenatum	1067:1135	Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum.					
31602875	3	40	theme	"	511:511	arg1	extraction					519:528	" water extraction	511:528	" water extraction	511:528	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	12	41	theme	alternative	1165:1175	arg1	method					1177:1182	an alternative method	1162:1182	an alternative method for quality control of D. catenatum	1162:1218	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	12	41	theme	alternative	1165:1175	arg1	it					1150:1151	it	1150:1151	it	1150:1151	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	2	42	theme	materials	260:268	arg1	quality					235:241	the quality	231:241	the quality of the medicinal materials	231:268	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	6	43	theme	Pharmacopoeia	763:775	arg1	method					741:746	method	741:746	method of the Chinese Pharmacopoeia( 2015 edition)	741:790	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	2	44	theme	medicinal	250:258	arg1	materials					260:268	the medicinal materials	246:268	the medicinal materials	246:268	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	6	45	theme	Chinese	755:761	arg1	Pharmacopoeia					763:775	the Chinese Pharmacopoeia	751:775	the Chinese Pharmacopoeia( 2015 edition)	751:790	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	6	45	theme	Chinese	755:761	arg1	edition					783:789	2015 edition	778:789	2015 edition	778:789	Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20.					
31602875	2	46	theme	quantification	312:325	arg1	method					327:332	a quantification method	310:332	a quantification method of non-starch polysaccharides	310:362	In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation.					
31602875	4	47	theme	starches	609:616	arg1	contents					597:604	The contents	593:604	The contents of starches,non-starch polysaccharides and monosaccharides	593:663	The contents of starches,non-starch polysaccharides and monosaccharides were analyzed.					
31602875	11	48	dep	D.	1124:1125	arg1	catenatum					1127:1135	D. catenatum	1124:1135	D. catenatum	1124:1135	Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum.					
31602875	10	49	theme	retained	949:956	arg1	polysaccharides					969:983	retained non-starch polysaccharides	949:983	retained non-starch polysaccharides	949:983	The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable.					
31602875	0	50	from	method	25:30	arg1	catenatum					76:84	Dendrobium catenatum	65:84	Dendrobium catenatum	65:84	[Study on quantification method of non-starch polysaccharides in Dendrobium catenatum].					
31602875	11	51	theme	polysaccharides	1105:1119	arg1	measurement					1079:1089	the content measurement	1067:1089	the content measurement of non-starch polysaccharides of D. catenatum	1067:1135	Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum.					
31602875	12	52	from	method	1177:1182	arg1	determination					1243:1255	the determination	1239:1255	the determination of non-starch polysaccharides in other starch-containing medicinal materials	1239:1332	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	3	53	theme	α-amylase	530:538	arg1	pretreatment					540:551	α-amylase pretreatment	530:551	α-amylase pretreatment	530:551	The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method.					
31602875	12	54	theme	quality	1188:1194	arg1	control					1196:1202	quality control	1188:1202	quality control of D. catenatum	1188:1218	Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.					
31602875	4	55	theme	non-starch	618:627	arg1	starches					609:616	starches	609:616	starches	609:616	The contents of starches,non-starch polysaccharides and monosaccharides were analyzed.					
31602875	4	55	theme	non-starch	618:627	arg1	polysaccharides					629:643	non-starch polysaccharides	618:643	non-starch polysaccharides	618:643	The contents of starches,non-starch polysaccharides and monosaccharides were analyzed.					
31835446	6	0	theme	Scanning	1012:1019	arg1	microscopy					1030:1039	Scanning electron microscopy	1012:1039	Scanning electron microscopy	1012:1039	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31835446	4	1	theme	weight	772:777	arg1	distribution					779:790	their molecular weight distribution	756:790	their molecular weight distribution	756:790	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	2	2	from	effect	312:317	arg1	adhesion					418:425	adhesion	418:425	adhesion	418:425	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	2	from	effect	312:317	arg1	serra					369:373	serra	369:373	serra	369:373	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	2	from	effect	312:317	arg1	verrucosa					390:398	verrucosa	390:398	verrucosa	390:398	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	2	from	effect	312:317	arg1	growth					407:412	growth	407:412	growth	407:412	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	6	3	theme	ETEC	1125:1128	arg1	adhesion					1134:1141	ETEC K88 adhesion	1125:1141	ETEC K88 adhesion	1125:1141	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31835446	0	4	from	Effect	11:16	arg1	Adhesion					94:101	Adhesion	94:101	Adhesion	94:101	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	4	from	Effect	11:16	arg1	Algae					70:74	Marine Red Algae	59:74	Marine Red Algae	59:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	4	from	Effect	11:16	arg1	Growth					83:88	Growth	83:88	Growth	83:88	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	7	5	theme	ETEC	1253:1256	arg1	K88					1258:1260	ETEC K88	1253:1260	ETEC K88	1253:1260	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	1	6	theme	safe	164:167	arg1	polymers					181:188	safe and natural polymers	164:188	safe and natural polymers against bacterial diarrhea	164:215	Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics.					
31835446	3	7	theme	weight	553:558	arg1	kDa					579:581	molecular weight distribution ≤20.0 kDa	543:581	molecular weight distribution ≤20.0 kDa	543:581	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	2	8	theme	inhibiting	301:310	arg1	effect					312:317	the inhibiting effect	297:317	the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	297:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	3	9	theme	ETEC	624:627	arg1	K88					629:631	ETEC K88	624:631	ETEC K88	624:631	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	5	10	with	treatment	971:979	arg1	polysaccharides					995:1009	selected polysaccharides	986:1009	selected polysaccharides	986:1009	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	2	11	from	serra	369:373	arg1	galactans					345:353	depolymerized sulfated galactans	322:353	depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa	322:398	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	11	from	serra	369:373	arg1	effect					312:317	the inhibiting effect	297:317	the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	297:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	3	12	theme	antibacterial	593:605	arg1	activity					607:614	antibacterial activity	593:614	antibacterial activity against ETEC K88	593:631	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	4	13	theme	sulfate	793:799	arg1	content					807:813	sulfate group content	793:813	sulfate group content	793:813	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	4	14	theme	molecular	762:770	arg1	distribution					779:790	their molecular weight distribution	756:790	their molecular weight distribution	756:790	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	4	15	theme	K88	689:691	arg1	activity					693:700	the anti-ETEC K88 activity	675:700	the anti-ETEC K88 activity of sulfated polysaccharides	675:728	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	5	16	theme	proteins	938:945	arg1	release					887:893	the promoted nucleic acid release	861:893	the promoted nucleic acid release	861:893	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	5	16	theme	proteins	938:945	arg1	quenching					916:924	the fluorescence quenching	899:924	the fluorescence quenching of membrane proteins	899:945	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	4	17	theme	structure-activity	636:653	arg1	study					655:659	A structure-activity study	634:659	A structure-activity study	634:659	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	5	18	theme	selected	986:993	arg1	polysaccharides					995:1009	selected polysaccharides	986:1009	selected polysaccharides	986:1009	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	7	19	theme	K88	1258:1260	arg1	adhesion					1241:1248	adhesion	1241:1248	adhesion	1241:1248	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	7	19	theme	K88	1258:1260	arg1	growth					1230:1235	growth	1230:1235	growth	1230:1235	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	1	20	theme	natural	173:179	arg1	polymers					181:188	safe and natural polymers	164:188	safe and natural polymers against bacterial diarrhea	164:215	Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics.					
31835446	5	21	theme	acid	882:885	arg1	release					887:893	the promoted nucleic acid release	861:893	the promoted nucleic acid release	861:893	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	2	22	theme	sulfated	336:343	arg1	galactans					345:353	depolymerized sulfated galactans	322:353	depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa	322:398	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	0	23	theme	Inhibitory	0:9	arg1	Effect					11:16	Inhibitory Effect	0:16	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.	0:136	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	7	24	from	effect	1216:1221	arg1	adhesion					1241:1248	adhesion	1241:1248	adhesion	1241:1248	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	7	24	from	effect	1216:1221	arg1	growth					1230:1235	growth	1230:1235	growth	1230:1235	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	3	25	with	polysaccharides	522:536	arg1	kDa					579:581	molecular weight distribution ≤20.0 kDa	543:581	molecular weight distribution ≤20.0 kDa	543:581	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	2	26	theme	depolymerized	322:334	arg1	galactans					345:353	depolymerized sulfated galactans	322:353	depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa	322:398	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	0	27	theme	Depolymerized	21:33	arg1	Galactans					44:52	Depolymerized Sulfated Galactans	21:52	Depolymerized Sulfated Galactans from Marine Red Algae	21:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	6	28	theme	sulfated	1082:1089	arg1	galactans					1091:1099	the depolymerized sulfated galactans	1064:1099	the depolymerized sulfated galactans	1064:1099	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31835446	2	29	from	verrucosa	390:398	arg1	galactans					345:353	depolymerized sulfated galactans	322:353	depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa	322:398	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	29	from	verrucosa	390:398	arg1	effect					312:317	the inhibiting effect	297:317	the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	297:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	4	30	theme	monosaccharide	820:833	arg1	composition					835:845	monosaccharide composition	820:845	monosaccharide composition	820:845	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	1	31	theme	bacterial	198:206	arg1	diarrhea					208:215	bacterial diarrhea	198:215	bacterial diarrhea	198:215	Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics.					
31835446	2	32	theme	coli	472:475	arg1	K88					484:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	6	33	theme	depolymerized	1068:1080	arg1	galactans					1091:1099	the depolymerized sulfated galactans	1064:1099	the depolymerized sulfated galactans	1064:1099	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31835446	7	34	theme	inhibitory	1205:1214	arg1	effect					1216:1221	an inhibitory effect	1202:1221	an inhibitory effect on the growth and adhesion of ETEC K88	1202:1260	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	3	35	theme	molecular	543:551	arg1	kDa					579:581	molecular weight distribution ≤20.0 kDa	543:581	molecular weight distribution ≤20.0 kDa	543:581	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	2	36	theme	enterotoxigenic	444:458	arg1	K88					484:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	0	37	theme	Galactans	44:52	arg1	Effect					11:16	Inhibitory Effect	0:16	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.	0:136	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	4	38	theme	sulfated	705:712	arg1	polysaccharides					714:728	sulfated polysaccharides	705:728	sulfated polysaccharides	705:728	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	2	39	theme	diarrheagenic	430:442	arg1	K88					484:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	430:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	0	40	theme	Sulfated	35:42	arg1	Galactans					44:52	Depolymerized Sulfated Galactans	21:52	Depolymerized Sulfated Galactans from Marine Red Algae	21:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	41	theme	coli	132:135	arg1	Adhesion					94:101	Adhesion	94:101	Adhesion	94:101	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	41	theme	coli	132:135	arg1	Growth					83:88	Growth	83:88	Growth	83:88	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	42	from	Algae	70:74	arg1	Galactans					44:52	Depolymerized Sulfated Galactans	21:52	Depolymerized Sulfated Galactans from Marine Red Algae	21:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	0	42	from	Algae	70:74	arg1	Effect					11:16	Inhibitory Effect	0:16	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.	0:136	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	5	43	theme	membrane	929:936	arg1	proteins					938:945	membrane proteins	929:945	membrane proteins	929:945	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	0	44	theme	Marine	59:64	arg1	Algae					70:74	Marine Red Algae	59:74	Marine Red Algae	59:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	5	45	theme	nucleic	874:880	arg1	release					887:893	the promoted nucleic acid release	861:893	the promoted nucleic acid release	861:893	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	5	46	located	observed	952:959	arg2	release					887:893	the promoted nucleic acid release	861:893	the promoted nucleic acid release	861:893	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	5	46	located	observed	952:959	arg2	quenching					916:924	the fluorescence quenching	899:924	the fluorescence quenching of membrane proteins	899:945	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	5	46	located	observed	952:959	arg1	addition					851:858	addition	851:858	addition	851:858	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	3	47	theme	sulfated	513:520	arg1	polysaccharides					522:536	the sulfated polysaccharides	509:536	the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa	509:581	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	2	48	theme	K88	484:486	arg1	adhesion					418:425	adhesion	418:425	adhesion	418:425	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	2	48	theme	K88	484:486	arg1	growth					407:412	growth	407:412	growth	407:412	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	7	49	theme	sulfated	1173:1180	arg1	galactans					1182:1190	depolymerized sulfated galactans	1159:1190	depolymerized sulfated galactans	1159:1190	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	5	50	theme	promoted	865:872	arg1	release					887:893	the promoted nucleic acid release	861:893	the promoted nucleic acid release	861:893	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	2	51	dep	growth	407:412	arg1	the					403:405	the	403:405	the	403:405	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	4	52	theme	group	801:805	arg1	content					807:813	sulfate group content	793:813	sulfate group content	793:813	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	2	53	theme	galactans	345:353	arg1	effect					312:317	the inhibiting effect	297:317	the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88	297:486	This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88.					
31835446	7	54	theme	depolymerized	1159:1171	arg1	galactans					1182:1190	depolymerized sulfated galactans	1159:1190	depolymerized sulfated galactans	1159:1190	In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.					
31835446	5	55	theme	fluorescence	903:914	arg1	quenching					916:924	the fluorescence quenching	899:924	the fluorescence quenching of membrane proteins	899:945	In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides.					
31835446	1	56	theme	Active	138:143	arg1	polysaccharides					145:159	Active polysaccharides	138:159	Active polysaccharides as safe and natural polymers against bacterial diarrhea	138:215	Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics.					
31835446	1	56	theme	Active	138:143	arg1	alternative					246:256	an alternative	243:256	an alternative to antibiotics	243:271	Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics.					
31835446	4	57	theme	polysaccharides	714:728	arg1	activity					693:700	the anti-ETEC K88 activity	675:700	the anti-ETEC K88 activity of sulfated polysaccharides	675:728	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	4	58	theme	anti-ETEC	679:687	arg1	activity					693:700	the anti-ETEC K88 activity	675:700	the anti-ETEC K88 activity of sulfated polysaccharides	675:728	A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition.					
31835446	0	59	theme	Red	66:68	arg1	Algae					70:74	Marine Red Algae	59:74	Marine Red Algae	59:74	Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.					
31835446	6	60	theme	electron	1021:1028	arg1	microscopy					1030:1039	Scanning electron microscopy	1012:1039	Scanning electron microscopy	1012:1039	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31835446	3	61	theme	distribution	560:571	arg1	kDa					579:581	molecular weight distribution ≤20.0 kDa	543:581	molecular weight distribution ≤20.0 kDa	543:581	Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88.					
31835446	6	62	theme	K88	1130:1132	arg1	adhesion					1134:1141	ETEC K88 adhesion	1125:1141	ETEC K88 adhesion	1125:1141	Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion.					
31342708	2	0	dep	flower	1562:1567	arg1	flower					1595:1600	flower	1595:1600	flower	1595:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	1	contain	has	122:124	arg1	flower					94:99	The flower	90:99	The flower of Hibiscus syriacus	90:120	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	1	contain	has	122:124	arg2	values.In					163:171	good ornamental and edible-medicinal values.In	126:171	good ornamental and edible-medicinal values.In	126:171	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	2	theme	dose-effect	1422:1432	arg1	relationship					1434:1445	a good dose-effect relationship	1415:1445	a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower	1415:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	3	theme	white	1573:1577	arg1	petals					1588:1593	white multiple petals	1573:1593	white multiple petals	1573:1593	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	4	theme	multiple	259:266	arg1	petals					268:273	pink multiple petals	254:273	pink multiple petals flower	254:280	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	5	theme	functional	1904:1913	arg1	foods					1915:1919	functional foods	1904:1919	functional foods	1904:1919	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	6	theme	flower	1562:1567	arg1	concentration					1456:1468	the concentration	1452:1468	the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower	1452:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	7	theme	flowers	741:747	arg1	h.Polysaccharides					709:725	3.1 h.Polysaccharides	705:725	3.1 h.Polysaccharides of H.syriacus flowers	705:747	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	8	theme	single	485:490	arg1	experiment					499:508	investigated.Through single factor experiment	464:508	investigated.Through single factor experiment	464:508	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	0	9	theme	[Composition	0:11	arg1	analysis					13:20	[Composition analysis	0:20	[Composition analysis	0:20	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	2	10	theme	ABTS	1361:1364	arg1	assay					1366:1370	ABTS assay	1361:1370	ABTS assay	1361:1370	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	11	theme	43.5∶1	668:673	arg1	m					675:675	m	675:675	m	675:675	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	12	theme	theoretical	1829:1839	arg1	basis					1841:1845	a theoretical basis	1827:1845	a theoretical basis for the application of H.syriacus flower in the field of functional foods	1827:1919	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	13	theme	petals	1588:1593	arg1	concentration					1456:1468	the concentration	1452:1468	the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower	1452:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	14	theme	reducing	1333:1340	arg1	assay					1351:1355	total reducing capacity assay	1327:1355	total reducing capacity assay	1327:1355	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	15	theme	L·g~	677:680	arg1	liquid					658:663	liquid	658:663	liquid	658:663	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	16	dep	masses	1625:1630	arg1	composition					1652:1662	composition	1652:1662	composition	1652:1662	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	17	theme	potential	1244:1252	arg1	activity					1266:1273	potential antioxidant activity	1244:1273	potential antioxidant activity	1244:1273	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	18	theme	antioxidant	409:419	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	18	theme	antioxidant	409:419	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	19	theme	flowers	950:956	arg1	respectively.Polysaccharides					907:934	respectively.Polysaccharides	907:934	respectively.Polysaccharides of H.syriacus flowers	907:956	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	20	theme	flowers	1229:1235	arg1	polysaccharides					1199:1213	crude polysaccharides	1193:1213	crude polysaccharides of H.syriacus flowers	1193:1235	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	21	with	relationship	1434:1445	arg1	concentration					1456:1468	the concentration	1452:1468	the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower	1452:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	22	theme	ornamental	131:140	arg1	values.In					163:171	good ornamental and edible-medicinal values.In	126:171	good ornamental and edible-medicinal values.In	126:171	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	23	theme	petals	1733:1738	arg1	flower					1740:1745	pink multiple petals flower	1719:1745	pink multiple petals flower	1719:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	24	theme	flowers.The	1781:1791	arg1	results					1793:1799	the white flowers.The results	1771:1799	the white flowers.The results of this study	1771:1813	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	25	theme	1,1-diphenyl-2-picrylhydrazyl	1278:1306	arg1	DPPH					1316:1319	DPPH	1316:1319	DPPH	1316:1319	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	25	theme	1,1-diphenyl-2-picrylhydrazyl	1278:1306	arg1	radical					1308:1314	1,1-diphenyl-2-picrylhydrazyl radical	1278:1314	1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay	1278:1325	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	26	from	polysaccharides	433:447	arg1	flower					452:457	flower	452:457	flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	452:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	27	theme	edible-medicinal	146:161	arg1	values.In					163:171	good ornamental and edible-medicinal values.In	126:171	good ornamental and edible-medicinal values.In	126:171	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	28	theme	molecular	814:822	arg1	masses					824:829	molecular masses	814:829	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	29	theme	material	646:653	arg1	ratio					637:641	ratio	637:641	ratio of material to liquid of 43.5∶1 m L·g~(-1)	637:684	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	29	theme	material	646:653	arg1	96.8℃					631:635	96.8℃	631:635	96.8℃	631:635	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	30	from	activity	421:428	arg1	flower					452:457	flower	452:457	flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	452:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	31	theme	extraction	330:339	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	31	theme	extraction	330:339	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	31	theme	extraction	330:339	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	31	theme	extraction	330:339	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	32	theme	study	1809:1813	arg1	results					1793:1799	the white flowers.The results	1771:1799	the white flowers.The results of this study	1771:1813	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	33	theme	tested	1505:1510	arg1	polysaccharides					1522:1536	polysaccharides	1522:1536	polysaccharides of pink multiple petals	1522:1560	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	33	theme	tested	1505:1510	arg1	varieties					1512:1520	the tested varieties	1501:1520	the tested varieties	1501:1520	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	34	dep	materials.And	304:316	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	34	dep	materials.And	304:316	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	34	dep	materials.And	304:316	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	34	dep	materials.And	304:316	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	35	theme	molecular	361:369	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	35	theme	molecular	361:369	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	36	theme	multiple	1546:1553	arg1	petals					1555:1560	pink multiple petals	1541:1560	pink multiple petals	1541:1560	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	37	theme	antioxidant	1672:1682	arg1	activity					1684:1691	the antioxidant activity	1668:1691	the antioxidant activity of the polysaccharides of pink multiple petals flower	1668:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	37	theme	antioxidant	1672:1682	arg1	higher					1751:1756	higher	1751:1756	higher	1751:1756	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	0	38	from	activity	38:45	arg1	syriacus					79:86	Hibiscus syriacus	70:86	Hibiscus syriacus	70:86	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	1	39	theme	samples	855:861	arg1	masses					824:829	molecular masses	814:829	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	40	from	weight	371:376	arg1	flower					452:457	flower	452:457	flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	452:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	41	theme	petals	268:273	arg1	varieties					204:212	two varieties	200:212	two varieties	200:212	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	41	theme	petals	268:273	arg1	flower					275:280	pink multiple petals flower	254:280	pink multiple petals flower	254:280	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	42	theme	pink	1719:1722	arg1	flower					1740:1745	pink multiple petals flower	1719:1745	pink multiple petals flower	1719:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	43	theme	crude	1193:1197	arg1	polysaccharides					1199:1213	crude polysaccharides	1193:1213	crude polysaccharides of H.syriacus flowers	1193:1235	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	44	theme	Hibiscus	104:111	arg1	syriacus					113:120	Hibiscus syriacus	104:120	Hibiscus syriacus	104:120	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	45	theme	polysaccharides	1700:1714	arg1	activity					1684:1691	the antioxidant activity	1668:1691	the antioxidant activity of the polysaccharides of pink multiple petals flower	1668:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	45	theme	polysaccharides	1700:1714	arg1	higher					1751:1756	higher	1751:1756	higher	1751:1756	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	46	theme	performance	771:781	arg1	chromatography.The					787:804	high performance gel chromatography.The	766:804	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	0	47	theme	Hibiscus	70:77	arg1	syriacus					79:86	Hibiscus syriacus	70:86	Hibiscus syriacus	70:86	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	0	48	theme	polysaccharide	50:63	arg1	analysis					13:20	[Composition analysis	0:20	[Composition analysis	0:20	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	0	48	theme	polysaccharide	50:63	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	1	49	theme	test	299:302	arg1	study					178:182	this study	173:182	this study	173:182	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	49	theme	test	299:302	arg1	samples					189:195	four samples	184:195	four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,	184:281	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	49	theme	test	299:302	arg1	materials.And					304:316	test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	299:1179	test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	299:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	50	theme	polysaccharides.Among	1479:1499	arg1	flower					1562:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	51	theme	molecular	1615:1623	arg1	masses					1625:1630	the same molecular masses	1606:1630	the same molecular masses	1606:1630	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	52	theme	chromatography.The	787:804	arg1	were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5					863:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	53	theme	pre-column	1034:1043	arg1	ratio					1069:1073	pre-column derivatization HPLC.The ratio	1034:1073	pre-column derivatization HPLC.The ratio of galactose	1034:1086	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	54	theme	extraction	686:695	arg1	time					697:700	extraction time	686:700	extraction time	686:700	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	54	theme	extraction	686:695	arg1	96.8℃					631:635	96.8℃	631:635	96.8℃	631:635	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	55	theme	extract	543:549	arg1	conditions					551:560	the optimal extract conditions	531:560	the optimal extract conditions of polysaccharide	531:578	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	56	theme	multiple	227:234	arg1	petals					236:241	white multiple petals	221:241	white multiple petals flower	221:248	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	57	theme	antioxidant	1389:1399	arg1	effect					1401:1406	its antioxidant effect	1385:1406	its antioxidant effect	1385:1406	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	58	theme	HPLC.The	1060:1067	arg1	ratio					1069:1073	pre-column derivatization HPLC.The ratio	1034:1073	pre-column derivatization HPLC.The ratio of galactose	1034:1086	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	59	theme	flower	1881:1886	arg1	application					1855:1865	the application	1851:1865	the application of H.syriacus flower in the field of functional foods	1851:1919	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	60	theme	multiple	1579:1586	arg1	petals					1588:1593	white multiple petals	1573:1593	white multiple petals	1573:1593	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	61	from	flower	452:457	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	61	from	flower	452:457	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	61	from	flower	452:457	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	61	from	flower	452:457	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	62	theme	h.Polysaccharides	709:725	arg1	temperature					616:626	extraction temperature	605:626	extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers	605:747	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	63	theme	pink	254:257	arg1	petals					268:273	pink multiple petals	254:273	pink multiple petals flower	254:280	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	64	theme	good	1417:1420	arg1	relationship					1434:1445	a good dose-effect relationship	1415:1445	a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower	1415:1600	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	65	theme	foods	1915:1919	arg1	field					1895:1899	the field	1891:1899	the field of functional foods	1891:1919	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	66	theme	H.syriacus	730:739	arg1	flowers					741:747	H.syriacus flowers	730:747	H.syriacus flowers	730:747	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	67	theme	investigated.Through	464:483	arg1	experiment					499:508	investigated.Through single factor experiment	464:508	investigated.Through single factor experiment	464:508	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	68	theme	factor	492:497	arg1	experiment					499:508	investigated.Through single factor experiment	464:508	investigated.Through single factor experiment	464:508	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	69	theme	radical	1308:1314	arg1	assay					1321:1325	1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay	1278:1325	1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay	1278:1325	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	70	theme	m	675:675	arg1	-1					682:683	-1	682:683	-1	682:683	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	70	theme	m	675:675	arg1	L·g~					677:680	43.5∶1 m L·g~	668:680	43.5∶1 m L·g~(-1)	668:684	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	71	theme	capacity	1342:1349	arg1	assay					1351:1355	total reducing capacity assay	1327:1355	total reducing capacity assay	1327:1355	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	72	dep	showed	1408:1413	arg1	had					1602:1604	had	1602:1604	showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition	1408:1662	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	73	from	96.8℃	631:635	arg1	temperature					616:626	extraction temperature	605:626	extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers	605:747	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	74	theme	total	1327:1331	arg1	assay					1351:1355	total reducing capacity assay	1327:1355	total reducing capacity assay	1327:1355	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	75	theme	monosaccharide	378:391	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	75	theme	monosaccharide	378:391	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	76	theme	varieties	204:212	arg1	study					178:182	this study	173:182	this study	173:182	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	76	theme	varieties	204:212	arg1	samples					189:195	four samples	184:195	four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,	184:281	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	76	theme	varieties	204:212	arg1	materials.And					304:316	test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	299:1179	test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	299:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	0	77	from	syriacus	79:86	arg1	polysaccharide					50:63	polysaccharide	50:63	polysaccharide from Hibiscus syriacus	50:86	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	0	77	from	syriacus	79:86	arg1	analysis					13:20	[Composition analysis	0:20	[Composition analysis	0:20	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	0	77	from	syriacus	79:86	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	1	78	theme	syriacus	113:120	arg1	flower					94:99	The flower	90:99	The flower of Hibiscus syriacus	90:120	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	79	theme	H.syriacus	939:948	arg1	flowers					950:956	H.syriacus flowers	939:956	H.syriacus flowers	939:956	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	80	theme	good	126:129	arg1	values.In					163:171	good ornamental and edible-medicinal values.In	126:171	good ornamental and edible-medicinal values.In	126:171	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	81	theme	flower	1740:1745	arg1	polysaccharides					1700:1714	the polysaccharides	1696:1714	the polysaccharides of pink multiple petals flower	1696:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	82	theme	H.syriacus	1218:1227	arg1	flowers					1229:1235	H.syriacus flowers	1218:1235	H.syriacus flowers	1218:1235	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	83	theme	polysaccharides	433:447	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	83	theme	polysaccharides	433:447	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	83	theme	polysaccharides	433:447	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	83	theme	polysaccharides	433:447	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	84	theme	multiple	1724:1731	arg1	flower					1740:1745	pink multiple petals flower	1719:1745	pink multiple petals flower	1719:1745	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	85	theme	white	1775:1779	arg1	results					1793:1799	the white flowers.The results	1771:1799	the white flowers.The results of this study	1771:1813	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	86	theme	optimum	322:328	arg1	composition					393:403	monosaccharide composition	378:403	monosaccharide composition	378:403	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	86	theme	optimum	322:328	arg1	activity					421:428	antioxidant activity	409:428	antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	409:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	86	theme	optimum	322:328	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	86	theme	optimum	322:328	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	87	theme	galactose	1144:1152	arg1	ratio					1135:1139	the ratio	1131:1139	the ratio of galactose to glucose	1131:1163	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	87	theme	galactose	1144:1152	arg1	1.656-4.496					1169:1179	1.656-4.496	1169:1179	1.656-4.496	1169:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	0	88	from	analysis	13:20	arg1	syriacus					79:86	Hibiscus syriacus	70:86	Hibiscus syriacus	70:86	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	1	89	theme	masses	824:829	arg1	were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5					863:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	90	theme	antioxidant	1254:1264	arg1	activity					1266:1273	potential antioxidant activity	1244:1273	potential antioxidant activity	1244:1273	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	91	from	composition	393:403	arg1	flower					452:457	flower	452:457	flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496	452:1179	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	92	theme	varieties	1512:1520	arg1	flower					1562:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	93	theme	polysaccharide	565:578	arg1	conditions					551:560	the optimal extract conditions	531:560	the optimal extract conditions of polysaccharide	531:578	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	94	theme	relative	352:359	arg1	conditions					341:350	the optimum extraction conditions	318:350	the optimum extraction conditions	318:350	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	94	theme	relative	352:359	arg1	weight					371:376	relative molecular weight	352:376	relative molecular weight	352:376	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	95	theme	petals	1555:1560	arg1	polysaccharides					1522:1536	polysaccharides	1522:1536	polysaccharides of pink multiple petals	1522:1560	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	95	theme	petals	1555:1560	arg1	varieties					1512:1520	the tested varieties	1501:1520	the tested varieties	1501:1520	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	96	theme	galactose	1078:1086	arg1	ratio					1069:1073	pre-column derivatization HPLC.The ratio	1034:1073	pre-column derivatization HPLC.The ratio of galactose	1034:1086	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	97	theme	polysaccharide	840:853	arg1	samples					855:861	the 4 polysaccharide samples	834:861	the 4 polysaccharide samples	834:861	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	98	theme	pink	1541:1544	arg1	petals					1555:1560	pink multiple petals	1541:1560	pink multiple petals	1541:1560	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	99	from	application	1855:1865	arg1	field					1895:1899	the field	1891:1899	the field of functional foods	1891:1919	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	0	100	theme	antioxidant	26:36	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	[Composition analysis and antioxidant activity of polysaccharide from Hibiscus syriacus].					
31342708	1	101	theme	extraction	605:614	arg1	temperature					616:626	extraction temperature	605:626	extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers	605:747	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	102	theme	high	766:769	arg1	chromatography.The					787:804	high performance gel chromatography.The	766:804	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	103	theme	response	514:521	arg1	surface					523:529	response surface	514:529	response surface	514:529	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	104	theme	gel	783:785	arg1	chromatography.The					787:804	high performance gel chromatography.The	766:804	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	2	105	theme	crude	1473:1477	arg1	flower					1562:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower	1473:1567	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	2	106	theme	same	1610:1613	arg1	masses					1625:1630	the same molecular masses	1606:1630	the same molecular masses	1606:1630	In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.					
31342708	1	107	theme	average	806:812	arg1	were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5					863:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5	766:905	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	108	theme	optimal	535:541	arg1	conditions					551:560	the optimal extract conditions	531:560	the optimal extract conditions of polysaccharide	531:578	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	109	theme	white	221:225	arg1	petals					236:241	white multiple petals	221:241	white multiple petals flower	221:248	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	110	theme	derivatization	1045:1058	arg1	ratio					1069:1073	pre-column derivatization HPLC.The ratio	1034:1073	pre-column derivatization HPLC.The ratio of galactose	1034:1086	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	111	theme	petals	236:241	arg1	varieties					204:212	two varieties	200:212	two varieties	200:212	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31342708	1	111	theme	petals	236:241	arg1	flower					243:248	white multiple petals flower	221:248	white multiple petals flower	221:248	The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.					
31766509	0	0	theme	Fucosylated	66:76	arg1	Sulfate					90:96	Natural Fucosylated Chondroitin Sulfate	58:96	Natural Fucosylated Chondroitin Sulfate	58:96	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	7	1	theme	fCS	1472:1474	arg1	analogues					1476:1484	such semi-synthetic fCS analogues	1452:1484	such semi-synthetic fCS analogues	1452:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	4	2	theme	effects	699:705	arg1	preference					715:724	the preference	711:724	the preference for new drugs not sourced from animal tissues	711:770	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	4	2	theme	effects	699:705	arg1	structure					658:666	its typically heterogeneous structure	630:666	its typically heterogeneous structure	630:666	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	4	2	theme	effects	699:705	arg1	detection					673:681	the detection	669:681	the detection of some adverse effects	669:705	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	7	3	theme	activity	1431:1438	arg1	evaluation					1399:1408	A preliminary in vitro evaluation	1376:1408	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues	1376:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	1	4	theme	chondroitin	165:175	arg1	glycosaminoglycan					196:212	a glycosaminoglycan	194:212	a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers	194:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	4	theme	chondroitin	165:175	arg1	fCS					186:188	fCS	186:188	fCS	186:188	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	4	theme	chondroitin	165:175	arg1	sulfate					177:183	Fucosylated chondroitin sulfate	153:183	Fucosylated chondroitin sulfate (fCS)	153:189	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	0	5	theme	Natural	58:64	arg1	Sulfate					90:96	Natural Fucosylated Chondroitin Sulfate	58:96	Natural Fucosylated Chondroitin Sulfate	58:96	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	3	6	from	mechanism	403:411	arg1	cascade					448:454	the blood coagulation cascade	426:454	the blood coagulation cascade with respect to heparin	426:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	4	7	theme	heterogeneous	834:846	arg1	species					864:870	safer and less heterogeneous non-natural fCS species	819:870	safer and less heterogeneous non-natural fCS species	819:870	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	3	8	from	retention	488:496	arg1	cascade					448:454	the blood coagulation cascade	426:454	the blood coagulation cascade with respect to heparin	426:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	4	9	theme	adverse	691:697	arg1	effects					699:705	some adverse effects	686:705	some adverse effects	686:705	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	1	10	theme	body	249:252	arg1	wall					254:257	the body wall	245:257	the body wall of sea cucumbers	245:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	0	11	theme	Sulfate	90:96	arg1	Regioisomer					43:53	a Semi-synthetic Regioisomer	26:53	a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units	26:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	5	12	theme	O-3	1119:1121	arg1	O-6					1061:1063	position O-6	1052:1063	position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1052:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	4	13	theme	animal	757:762	arg1	tissues					764:770	animal tissues	757:770	animal tissues	757:770	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	5	14	theme	natural	1163:1169	arg1	fCSs					1171:1174	natural fCSs	1163:1174	natural fCSs	1163:1174	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	0	15	theme	Chondroitin	78:88	arg1	Sulfate					90:96	Natural Fucosylated Chondroitin Sulfate	58:96	Natural Fucosylated Chondroitin Sulfate	58:96	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	5	16	theme	N-acetyl-d-galactosamine	1068:1091	arg1	units					1102:1106	N-acetyl-d-galactosamine (GalNAc) units	1068:1106	N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1068:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	4	17	theme	fCS	860:862	arg1	species					864:870	safer and less heterogeneous non-natural fCS species	819:870	safer and less heterogeneous non-natural fCS species	819:870	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	2	18	theme	antithrombotic	345:358	arg1	ones					383:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	It shows several interesting activities, with the anticoagulant and antithrombotic as the most attractive ones.					
31766509	0	19	theme	Fucosyl	103:109	arg1	Branches					111:118	Fucosyl Branches	103:118	Fucosyl Branches on N-acetyl-Galactosamine Units	103:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	4	20	theme	new	730:732	arg1	drugs					734:738	new drugs	730:738	new drugs not sourced from animal tissues	730:770	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	4	21	theme	safer	819:823	arg1	species					864:870	safer and less heterogeneous non-natural fCS species	819:870	safer and less heterogeneous non-natural fCS species	819:870	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	0	22	with	Regioisomer	43:53	arg1	Branches					111:118	Fucosyl Branches	103:118	Fucosyl Branches on N-acetyl-Galactosamine Units	103:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	3	23	theme	action	416:421	arg1	mechanism					403:411	Its different mechanism	389:411	Its different mechanism of action on the blood coagulation cascade with respect to heparin	389:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	3	23	theme	action	416:421	arg1	retention					488:496	the retention	484:496	the retention of its activity by oral administration	484:535	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	4	24	theme	non-natural	848:858	arg1	species					864:870	safer and less heterogeneous non-natural fCS species	819:870	safer and less heterogeneous non-natural fCS species	819:870	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	6	25	theme	branches	1320:1327	arg1	insertion					1299:1307	regioselective insertion	1284:1307	regioselective insertion of fucosyl branches	1284:1327	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	6	25	theme	branches	1320:1327	arg1	branches					1320:1327	fucosyl branches	1312:1327	fucosyl branches	1312:1327	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	6	25	theme	branches	1320:1327	arg1	groups					1341:1346	sulfate groups	1333:1346	sulfate groups on its polymeric structure	1333:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	5	26	theme	GalNAc	1094:1099	arg1	units					1102:1106	N-acetyl-d-galactosamine (GalNAc) units	1068:1106	N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1068:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	3	27	theme	oral	517:520	arg1	administration					522:535	oral administration	517:535	oral administration	517:535	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	7	28	theme	analogues	1476:1484	arg1	analogues					1476:1484	such semi-synthetic fCS analogues	1452:1484	such semi-synthetic fCS analogues	1452:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	7	28	theme	analogues	1476:1484	arg1	three					1443:1447	three	1443:1447	three	1443:1447	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	3	29	theme	blood	430:434	arg1	cascade					448:454	the blood coagulation cascade	426:454	the blood coagulation cascade with respect to heparin	426:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	6	30	theme	microbial	1236:1244	arg1	polysaccharide					1266:1279	a microbial sourced chondroitin polysaccharide	1234:1279	a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure	1234:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	7	31	theme	semi-synthetic	1457:1470	arg1	analogues					1476:1484	such semi-synthetic fCS analogues	1452:1484	such semi-synthetic fCS analogues	1452:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	6	32	from	insertion	1299:1307	arg1	structure					1365:1373	its polymeric structure	1351:1373	its polymeric structure	1351:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	7	33	theme	preliminary	1378:1388	arg1	evaluation					1399:1408	A preliminary in vitro evaluation	1376:1408	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues	1376:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	7	34	theme	three	1443:1447	arg1	activity					1431:1438	the anticoagulant activity	1413:1438	the anticoagulant activity of three of such semi-synthetic fCS analogues	1413:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	2	35	theme	attractive	372:381	arg1	ones					383:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	It shows several interesting activities, with the anticoagulant and antithrombotic as the most attractive ones.					
31766509	1	36	theme	sea	262:264	arg1	cucumbers					266:274	sea cucumbers	262:274	sea cucumbers	262:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	7	37	theme	in	1390:1391	arg1	evaluation					1399:1408	A preliminary in vitro evaluation	1376:1408	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues	1376:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	2	38	theme	interesting	294:304	arg1	activities					306:315	several interesting activities	286:315	several interesting activities	286:315	It shows several interesting activities, with the anticoagulant and antithrombotic as the most attractive ones.					
31766509	5	39	theme	position	1052:1059	arg1	O-6					1061:1063	position O-6	1052:1063	position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1052:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	1	40	theme	cucumbers	266:274	arg1	wall					254:257	the body wall	245:257	the body wall of sea cucumbers	245:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	6	41	theme	fucosyl	1312:1318	arg1	branches					1320:1327	fucosyl branches	1312:1327	fucosyl branches	1312:1327	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	2	42	theme	several	286:292	arg1	activities					306:315	several interesting activities	286:315	several interesting activities	286:315	It shows several interesting activities, with the anticoagulant and antithrombotic as the most attractive ones.					
31766509	3	43	theme	promising	553:561	arg1	candidate					582:590	a very promising anticoagulant drug candidate	546:590	a very promising anticoagulant drug candidate for heparin replacement	546:614	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	5	44	theme	ones	1151:1154	arg1	O-3					1119:1121	O-3	1119:1121	N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1068:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	5	45	theme	chemical	932:939	arg1	strategy					941:948	a suitable chemical strategy	921:948	a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs	921:1174	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	7	46	theme	anticoagulant	1417:1429	arg1	activity					1431:1438	the anticoagulant activity	1413:1438	the anticoagulant activity of three of such semi-synthetic fCS analogues	1413:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	4	47	theme	heterogeneous	644:656	arg1	structure					658:666	its typically heterogeneous structure	630:666	its typically heterogeneous structure	630:666	Nonetheless, its typically heterogeneous structure, the detection of some adverse effects and the preference for new drugs not sourced from animal tissues, explain how mandatory is to open an access to safer and less heterogeneous non-natural fCS species.					
31766509	5	48	theme	fCS	989:991	arg1	polysaccharide					993:1006	the natural fCS polysaccharide	977:1006	the natural fCS polysaccharide	977:1006	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	3	49	theme	coagulation	436:446	arg1	cascade					448:454	the blood coagulation cascade	426:454	the blood coagulation cascade with respect to heparin	426:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	0	50	theme	N-acetyl-Galactosamine	123:144	arg1	Units					146:150	N-acetyl-Galactosamine Units	123:150	N-acetyl-Galactosamine Units	123:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	5	51	theme	polysaccharide	993:1006	arg1	regioisomer					962:972	a regioisomer	960:972	a regioisomer of the natural fCS polysaccharide	960:1006	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	0	52	theme	Semi-synthetic	28:41	arg1	Regioisomer					43:53	a Semi-synthetic Regioisomer	26:53	a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units	26:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
31766509	5	53	theme	d-glucuronic	1126:1137	arg1	acid					1139:1142	d-glucuronic acid	1126:1142	d-glucuronic acid (GlcA) ones	1126:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	5	53	theme	d-glucuronic	1126:1137	arg1	GlcA					1145:1148	GlcA	1145:1148	GlcA	1145:1148	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	1	54	dep	now	226:228	arg1	up					220:221	up	220:221	up	220:221	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	3	55	theme	anticoagulant	563:575	arg1	candidate					582:590	a very promising anticoagulant drug candidate	546:590	a very promising anticoagulant drug candidate for heparin replacement	546:614	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	3	56	from	cascade	448:454	arg1	mechanism					403:411	Its different mechanism	389:411	Its different mechanism of action on the blood coagulation cascade with respect to heparin	389:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	3	56	from	cascade	448:454	arg1	retention					488:496	the retention	484:496	the retention of its activity by oral administration	484:535	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	2	57	theme	anticoagulant	327:339	arg1	ones					383:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	the anticoagulant and antithrombotic as the most attractive ones	323:386	It shows several interesting activities, with the anticoagulant and antithrombotic as the most attractive ones.					
31766509	3	58	theme	different	393:401	arg1	mechanism					403:411	Its different mechanism	389:411	Its different mechanism of action on the blood coagulation cascade with respect to heparin	389:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	5	59	theme	acid	1139:1142	arg1	ones					1151:1154	d-glucuronic acid (GlcA) ones	1126:1154	d-glucuronic acid (GlcA) ones	1126:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	6	60	theme	polysaccharide	1266:1279	arg1	modification					1218:1229	the structural modification	1203:1229	the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure	1203:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	5	61	theme	sulfated	1014:1021	arg1	branches					1033:1040	sulfated l-fucosyl branches	1014:1040	sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1014:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	3	62	theme	drug	577:580	arg1	candidate					582:590	a very promising anticoagulant drug candidate	546:590	a very promising anticoagulant drug candidate for heparin replacement	546:614	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	6	63	theme	chondroitin	1254:1264	arg1	polysaccharide					1266:1279	a microbial sourced chondroitin polysaccharide	1234:1279	a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure	1234:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	5	64	theme	l-fucosyl	1023:1031	arg1	branches					1033:1040	sulfated l-fucosyl branches	1014:1040	sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1014:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	7	65	dep	in	1390:1391	arg1	vitro					1393:1397	vitro	1393:1397	vitro	1393:1397	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	6	66	from	groups	1341:1346	arg1	structure					1365:1373	its polymeric structure	1351:1373	its polymeric structure	1351:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	7	67	theme	such	1452:1455	arg1	analogues					1476:1484	such semi-synthetic fCS analogues	1452:1484	such semi-synthetic fCS analogues	1452:1484	A preliminary in vitro evaluation of the anticoagulant activity of three of such semi-synthetic fCS analogues is also reported.					
31766509	5	68	theme	suitable	923:930	arg1	strategy					941:948	a suitable chemical strategy	921:948	a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs	921:1174	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	6	69	theme	regioselective	1284:1297	arg1	insertion					1299:1307	regioselective insertion	1284:1307	regioselective insertion of fucosyl branches	1284:1327	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	6	70	theme	sourced	1246:1252	arg1	polysaccharide					1266:1279	a microbial sourced chondroitin polysaccharide	1234:1279	a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure	1234:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	6	71	theme	structural	1207:1216	arg1	modification					1218:1229	the structural modification	1203:1229	the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure	1203:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	3	72	from	action	416:421	arg1	cascade					448:454	the blood coagulation cascade	426:454	the blood coagulation cascade with respect to heparin	426:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	3	73	theme	activity	505:512	arg1	mechanism					403:411	Its different mechanism	389:411	Its different mechanism of action on the blood coagulation cascade with respect to heparin	389:478	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	3	73	theme	activity	505:512	arg1	retention					488:496	the retention	484:496	the retention of its activity by oral administration	484:535	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	6	74	theme	sulfate	1333:1339	arg1	branches					1320:1327	fucosyl branches	1312:1327	fucosyl branches	1312:1327	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	6	74	theme	sulfate	1333:1339	arg1	groups					1341:1346	sulfate groups	1333:1346	sulfate groups on its polymeric structure	1333:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	1	75	located	found	214:218	arg2	sulfate					177:183	Fucosylated chondroitin sulfate	153:183	Fucosylated chondroitin sulfate (fCS)	153:189	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	75	located	found	214:218	arg2	glycosaminoglycan					196:212	a glycosaminoglycan	194:212	a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers	194:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	75	located	found	214:218	arg1	wall					254:257	the body wall	245:257	the body wall of sea cucumbers	245:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	3	76	theme	heparin	596:602	arg1	replacement					604:614	heparin replacement	596:614	heparin replacement	596:614	Its different mechanism of action on the blood coagulation cascade with respect to heparin and the retention of its activity by oral administration make fCS a very promising anticoagulant drug candidate for heparin replacement.					
31766509	6	77	theme	polymeric	1355:1363	arg1	structure					1365:1373	its polymeric structure	1351:1373	its polymeric structure	1351:1373	This strategy is based on the structural modification of a microbial sourced chondroitin polysaccharide by regioselective insertion of fucosyl branches and sulfate groups on its polymeric structure.					
31766509	5	78	theme	units	1102:1106	arg1	O-6					1061:1063	position O-6	1052:1063	position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones	1052:1154	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	5	79	theme	natural	981:987	arg1	polysaccharide					993:1006	the natural fCS polysaccharide	977:1006	the natural fCS polysaccharide	977:1006	Here we contribute to this aim by investigating a suitable chemical strategy to obtain a regioisomer of the natural fCS polysaccharide, with sulfated l-fucosyl branches placed at position O-6 of N-acetyl-d-galactosamine (GalNAc) units instead of O-3 of d-glucuronic acid (GlcA) ones, as in natural fCSs.					
31766509	1	80	theme	Fucosylated	153:163	arg1	glycosaminoglycan					196:212	a glycosaminoglycan	194:212	a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers	194:274	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	80	theme	Fucosylated	153:163	arg1	fCS					186:188	fCS	186:188	fCS	186:188	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	1	80	theme	Fucosylated	153:163	arg1	sulfate					177:183	Fucosylated chondroitin sulfate	153:183	Fucosylated chondroitin sulfate (fCS)	153:189	Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan found up to now exclusively in the body wall of sea cucumbers.					
31766509	0	81	from	Branches	111:118	arg1	Units					146:150	N-acetyl-Galactosamine Units	123:150	N-acetyl-Galactosamine Units	123:150	A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units.					
30309594	0	0	theme	taste	101:105	arg1	profile					107:113	its taste profile	97:113	its taste profile	97:113	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile.					
30309594	7	1	theme	significant	1201:1211	arg1	improvement					1213:1223	significant improvement	1201:1223	significant improvement of edulcorant/organoleptic properties	1201:1261	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	1	2	theme	typical	171:177	arg1	effects					135:141	The adverse health effects	116:141	The adverse health effects	116:141	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	3	3	from	glycoside	430:438	arg1	plant					461:465	Stevia rebaudiana plant	443:465	Stevia rebaudiana plant leaves	443:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	6	4	theme	products	877:884	arg1	Structures					857:866	Structures	857:866	Structures of novel products	857:884	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	7	5	from	reduction	1140:1148	arg1	bitterness					1153:1162	bitterness	1153:1162	bitterness	1153:1162	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	5	6	theme	stevioside	766:775	arg1	α-glucosylation					747:761	the optimized and efficient α-glucosylation	719:761	the optimized and efficient α-glucosylation of stevioside	719:775	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	2	7	used	used	346:349	arg2	glycosides					318:327	steviol glycosides	310:327	steviol glycosides	310:327	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	2	7	used	used	346:349	arg2	sweeteners					369:378	high-intensity sweeteners	354:378	high-intensity sweeteners in food	354:386	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	7	8	theme	Sensory	1039:1045	arg1	analysis					1047:1054	Sensory analysis	1039:1054	Sensory analysis of the α-glucosylated stevioside products by a trained panel	1039:1115	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	6	9	theme	novel	871:875	arg1	products					877:884	novel products	871:884	novel products	871:884	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	4	10	theme	stevioside	611:620	arg1	bitterness					622:631	stevioside bitterness	611:631	stevioside bitterness	611:631	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	3	11	theme	food	516:519	arg1	products					521:528	food products	516:528	food products	516:528	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	3	12	theme	limited	492:498	arg1	applications					500:511	limited applications	492:511	limited applications in food products due to its lingering bitterness	492:560	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	2	13	theme	high-intensity	354:367	arg1	glycosides					318:327	steviol glycosides	310:327	steviol glycosides	310:327	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	2	13	theme	high-intensity	354:367	arg1	sweeteners					369:378	high-intensity sweeteners	354:378	high-intensity sweeteners in food	354:386	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	1	14	theme	developed	192:200	arg1	countries					202:210	developed countries	192:210	developed countries	192:210	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	0	15	theme	glucansucrase	50:62	arg1	Gtf180-ΔN-Q1140E					71:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile.					
30309594	6	16	theme	methylation	945:955	arg1	analysis					957:964	methylation analysis	945:964	methylation analysis	945:964	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	7	17	theme	properties	1252:1261	arg1	improvement					1213:1223	significant improvement	1201:1223	significant improvement of edulcorant/organoleptic properties	1201:1261	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	3	18	theme	lingering	541:549	arg1	bitterness					551:560	its lingering bitterness	537:560	its lingering bitterness	537:560	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	4	19	theme	low	683:685	arg1	productivities					687:700	low productivities	683:700	low productivities	683:700	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	2	20	from	sweeteners	369:378	arg1	food					383:386	food	383:386	food	383:386	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	3	21	theme	Stevia	443:448	arg1	plant					461:465	Stevia rebaudiana plant	443:465	Stevia rebaudiana plant leaves	443:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	3	22	from	applications	500:511	arg1	products					521:528	food products	516:528	food products	516:528	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	3	23	theme	rebaudiana	450:459	arg1	plant					461:465	Stevia rebaudiana plant	443:465	Stevia rebaudiana plant leaves	443:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	4	24	theme	Enzymatic	563:571	arg1	glucosylation					573:585	Enzymatic glucosylation	563:585	Enzymatic glucosylation	563:585	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	4	24	theme	Enzymatic	563:571	arg1	strategy					592:599	a strategy	590:599	a strategy to reduce stevioside bitterness	590:631	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	2	25	theme	steviol	310:316	arg1	glycosides					318:327	steviol glycosides	310:327	steviol glycosides	310:327	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	2	25	theme	steviol	310:316	arg1	sweeteners					369:378	high-intensity sweeteners	354:378	high-intensity sweeteners in food	354:386	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	0	26	theme	stevioside	25:34	arg1	Trans-α-glucosylation					0:20	Trans-α-glucosylation	0:20	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	0:86	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile.					
30309594	5	27	theme	efficient	737:745	arg1	α-glucosylation					747:761	the optimized and efficient α-glucosylation	719:761	the optimized and efficient α-glucosylation of stevioside	719:775	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	1	28	theme	adverse	120:126	arg1	effects					135:141	The adverse health effects	116:141	The adverse health effects	116:141	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	4	29	theme	glucosylation	647:659	arg1	reactions					661:669	reported glucosylation reactions	638:669	reported glucosylation reactions	638:669	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	0	30	theme	mutant	43:48	arg1	Gtf180-ΔN-Q1140E					71:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile.					
30309594	6	31	theme	glucosyl	1022:1029	arg1	moiety					1031:1036	the steviol C-19 glucosyl moiety	1005:1036	the steviol C-19 glucosyl moiety	1005:1036	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	7	32	theme	significant	1128:1138	arg1	reduction					1140:1148	a significant reduction	1126:1148	a significant reduction in bitterness	1126:1162	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	6	33	theme	mass	923:926	arg1	spectrometry					928:939	mass spectrometry	923:939	mass spectrometry	923:939	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	5	34	theme	donor	840:844	arg1	substrate					846:854	donor substrate	840:854	donor substrate	840:854	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	6	35	theme	C-19	1017:1020	arg1	moiety					1031:1036	the steviol C-19 glucosyl moiety	1005:1036	the steviol C-19 glucosyl moiety	1005:1036	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	5	36	theme	mutant	787:792	arg1	Gtf180-ΔN-Q1140E					808:823	the mutant glucansucrase Gtf180-ΔN-Q1140E	783:823	the mutant glucansucrase Gtf180-ΔN-Q1140E	783:823	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	0	37	theme	enzyme	64:69	arg1	Gtf180-ΔN-Q1140E					71:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E	39:86	Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile.					
30309594	6	38	theme	steviol	1009:1015	arg1	moiety					1031:1036	the steviol C-19 glucosyl moiety	1005:1036	the steviol C-19 glucosyl moiety	1005:1036	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	3	39	dep	plant	461:465	arg1	leaves					467:472	leaves	467:472	leaves	467:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	7	40	theme	trained	1103:1109	arg1	panel					1111:1115	a trained panel	1101:1115	a trained panel	1101:1115	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	1	41	theme	health	128:133	arg1	effects					135:141	The adverse health effects	116:141	The adverse health effects	116:141	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	3	42	theme	steviol	422:428	arg1	Stevioside					389:398	Stevioside	389:398	Stevioside	389:398	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	3	42	theme	steviol	422:428	arg1	glycoside					430:438	the most prevalent steviol glycoside	403:438	the most prevalent steviol glycoside in Stevia rebaudiana plant leaves	403:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	6	43	theme	NMR	905:907	arg1	spectroscopy					909:920	NMR spectroscopy	905:920	NMR spectroscopy	905:920	Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety.					
30309594	7	44	theme	stevioside	1078:1087	arg1	products					1089:1096	the α-glucosylated stevioside products	1059:1096	the α-glucosylated stevioside products	1059:1096	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	7	45	theme	edulcorant/organoleptic	1228:1250	arg1	properties					1252:1261	edulcorant/organoleptic properties	1228:1261	edulcorant/organoleptic properties	1228:1261	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	2	46	theme	European	282:289	arg1	2011					303:306	2011	303:306	2011	303:306	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	2	46	theme	European	282:289	arg1	Commission					291:300	the European Commission	278:300	the European Commission (2011)	278:307	Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food.					
30309594	7	47	theme	products	1089:1096	arg1	analysis					1047:1054	Sensory analysis	1039:1054	Sensory analysis of the α-glucosylated stevioside products by a trained panel	1039:1115	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	1	48	theme	low-calorie	232:242	arg1	sweeteners					244:253	low-calorie sweeteners	232:253	low-calorie sweeteners	232:253	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	5	49	theme	optimized	723:731	arg1	α-glucosylation					747:761	the optimized and efficient α-glucosylation	719:761	the optimized and efficient α-glucosylation of stevioside	719:775	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	1	50	theme	sucrose	146:152	arg1	overconsumption					154:168	sucrose overconsumption	146:168	sucrose overconsumption	146:168	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	1	51	theme	sweeteners	244:253	arg1	use					225:227	use	225:227	use of low-calorie sweeteners	225:253	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	5	52	theme	glucansucrase	794:806	arg1	Gtf180-ΔN-Q1140E					808:823	the mutant glucansucrase Gtf180-ΔN-Q1140E	783:823	the mutant glucansucrase Gtf180-ΔN-Q1140E	783:823	Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate.					
30309594	7	53	theme	α-glucosylated	1063:1076	arg1	products					1089:1096	the α-glucosylated stevioside products	1059:1096	the α-glucosylated stevioside products	1059:1096	Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties.					
30309594	1	54	theme	overconsumption	154:168	arg1	effects					135:141	The adverse health effects	116:141	The adverse health effects	116:141	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30309594	4	55	theme	reported	638:645	arg1	reactions					661:669	reported glucosylation reactions	638:669	reported glucosylation reactions	638:669	Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities.					
30309594	3	56	theme	prevalent	412:420	arg1	Stevioside					389:398	Stevioside	389:398	Stevioside	389:398	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	3	56	theme	prevalent	412:420	arg1	glycoside					430:438	the most prevalent steviol glycoside	403:438	the most prevalent steviol glycoside in Stevia rebaudiana plant leaves	403:472	Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness.					
30309594	1	57	from	diets	183:187	arg1	countries					202:210	developed countries	192:210	developed countries	192:210	The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners.					
30908644	1	0	theme	cellulose-52	350:361	arg1	chromatography					378:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	7	1	theme	friendly	1433:1440	arg1	technology					1499:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology	1414:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum	1414:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	6	2	theme	degradation	1240:1250	arg1	approach					1252:1259	the proposed microwave-assisted degradation approach	1208:1259	the proposed microwave-assisted degradation approach	1208:1259	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	6	2	theme	degradation	1240:1250	arg1	methodology					1280:1290	an efficacious methodology	1265:1290	an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides	1265:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	1	3	theme	Polygonatum	207:217	arg1	PSP					245:247	PSP	245:247	PSP	245:247	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	3	theme	Polygonatum	207:217	arg1	polysaccharide					229:242	crude Polygonatum sibiricum polysaccharide	201:242	crude Polygonatum sibiricum polysaccharide (PSP)	201:248	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	7	4	theme	microwave-assisted	1468:1485	arg1	technology					1499:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology	1414:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum	1414:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	6	5	theme	proposed	1212:1219	arg1	approach					1252:1259	the proposed microwave-assisted degradation approach	1208:1259	the proposed microwave-assisted degradation approach	1208:1259	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	6	5	theme	proposed	1212:1219	arg1	methodology					1280:1290	an efficacious methodology	1265:1290	an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides	1265:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	4	6	theme	antioxidant	865:875	arg1	activity					877:884	the lowest antioxidant activity	854:884	the lowest antioxidant activity	854:884	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	3	7	theme	metals	769:774	arg1	menchanism					683:692	the electron transfer menchanism	661:692	the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays)	661:739	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	7	theme	metals	769:774	arg1	chelation					745:753	chelation	745:753	chelation of transition metals (Fe2+ and Cu2+ chelation ability)	745:808	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	7	8	dep	APPLICATION	1381:1391	arg1	provided					1405:1412	provided	1405:1412	provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum	1405:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	4	9	theme	P-4	931:933	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	9	theme	P-4	931:933	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	10	theme	P-3	937:939	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	10	theme	P-3	937:939	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	7	11	theme	PRACTICAL	1371:1379	arg1	APPLICATION					1381:1391	PRACTICAL APPLICATION	1371:1391	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.	1371:1575	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	1	12	theme	diethylaminoethyl	332:348	arg1	chromatography					378:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	3	13	theme	fractions	634:642	arg1	activity					601:608	The antioxidant activity	585:608	The antioxidant activity of four polysaccharides fractions	585:642	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	1	14	theme	anion-exchange	363:376	arg1	chromatography					378:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	diethylaminoethyl cellulose-52 anion-exchange chromatography	332:391	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	4	15	theme	capacity	918:925	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	15	theme	capacity	918:925	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	1	16	dep	Polygonatum	207:217	arg1	sibiricum					219:227	sibiricum	219:227	sibiricum	219:227	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	5	17	theme	natural	1166:1172	arg1	P-1					1174:1176	natural P-1	1166:1176	natural P-1	1166:1176	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	6	18	theme	efficacious	1268:1278	arg1	approach					1252:1259	the proposed microwave-assisted degradation approach	1208:1259	the proposed microwave-assisted degradation approach	1208:1259	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	6	18	theme	efficacious	1268:1278	arg1	methodology					1280:1290	an efficacious methodology	1265:1290	an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides	1265:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	1	19	attach	isolated	187:194	arg2	P-1					133:135	P-1	133:135	P-1	133:135	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	19	attach	isolated	187:194	arg2	fractions					122:130	Four polysaccharide fractions	102:130	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%)	102:180	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	19	attach	isolated	187:194	arg1	PSP					245:247	PSP	245:247	PSP	245:247	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	19	attach	isolated	187:194	arg1	polysaccharide					229:242	crude Polygonatum sibiricum polysaccharide	201:242	crude Polygonatum sibiricum polysaccharide (PSP)	201:248	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	0	20	theme	Antioxidant	80:90	arg1	Activity					92:99	Antioxidant Activity	80:99	Antioxidant Activity	80:99	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	1	21	theme	water	264:268	arg1	extraction					270:279	water extraction	264:279	water extraction	264:279	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	21	theme	water	264:268	arg1	precipitation					290:302	ethanol precipitation	282:302	ethanol precipitation	282:302	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	2	22	theme	performance	477:487	arg1	chromatography					493:506	high performance gel chromatography	472:506	high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector	472:582	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	6	23	theme	polysaccharides	1354:1368	arg1	weight					1344:1349	the molecular weight	1330:1349	the molecular weight of polysaccharides	1330:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	3	24	dep	metals	769:774	arg1	metals					769:774	transition metals	758:774	transition metals (Fe2+ and Cu2+ chelation ability)	758:808	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	24	dep	metals	769:774	arg1	Cu2+					786:789	Cu2+	786:789	Cu2+	786:789	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	24	dep	metals	769:774	arg1	Fe2+					777:780	Fe2+	777:780	Fe2+	777:780	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	4	25	theme	P-2	943:945	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	25	theme	P-2	943:945	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	3	26	dep	Fe2+	777:780	arg1	ability					801:807	chelation ability	791:807	chelation ability	791:807	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	6	27	dep	weight	1344:1349	arg1	lower					1324:1328	lower	1324:1328	lower	1324:1328	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	2	28	with	chromatography	493:506	arg1	detector					575:582	ultraviolet-visible detector	555:582	ultraviolet-visible detector	555:582	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	2	28	with	chromatography	493:506	arg1	scattering					531:540	evaporative light scattering detector	513:549	evaporative light scattering detector	513:549	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	2	29	theme	evaporative	513:523	arg1	scattering					531:540	evaporative light scattering detector	513:549	evaporative light scattering detector	513:549	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	0	30	from	sibiricum	66:74	arg1	Degradation					19:29	Microwave-Assisted Degradation	0:29	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.	0:100	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	2	31	theme	monosaccharide	422:435	arg1	compositions					437:448	monosaccharide compositions	422:448	monosaccharide compositions	422:448	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	0	32	theme	Microwave-Assisted	0:17	arg1	Degradation					19:29	Microwave-Assisted Degradation	0:29	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.	0:100	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	5	33	theme	P-1	1130:1132	arg1	higher					1154:1159	higher	1154:1159	higher	1154:1159	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	5	33	theme	P-1	1130:1132	arg1	activity					1109:1116	the antioxidant activity	1093:1116	the antioxidant activity of degraded P-1	1093:1132	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	8	34	used	used	1598:1601	arg2	kind					1677:1680	a kind	1675:1680	a kind of food supplement in our daily life	1675:1717	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	8	34	used	used	1598:1601	arg2	results					1581:1587	The results	1577:1587	The results	1577:1587	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	4	35	theme	content	823:829	arg1	P-1					840:842	The highest content fraction P-1	811:842	The highest content fraction P-1	811:842	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	5	36	theme	antioxidant	1097:1107	arg1	higher					1154:1159	higher	1154:1159	higher	1154:1159	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	5	36	theme	antioxidant	1097:1107	arg1	activity					1109:1116	the antioxidant activity	1093:1116	the antioxidant activity of degraded P-1	1093:1132	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	3	37	theme	transfer	674:681	arg1	menchanism					683:692	the electron transfer menchanism	661:692	the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays)	661:739	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	38	theme	reducing	708:715	arg1	power					717:721	ferric reducing power	701:721	ferric reducing power	701:721	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	8	39	theme	supplement	1690:1699	arg1	results					1581:1587	The results	1577:1587	The results	1577:1587	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	8	39	theme	supplement	1690:1699	arg1	kind					1677:1680	a kind	1675:1680	a kind of food supplement in our daily life	1675:1717	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	3	40	dep	menchanism	683:692	arg1	power					717:721	ferric reducing power	701:721	ferric reducing power	701:721	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	40	dep	menchanism	683:692	arg1	assays					733:738	ABST assays	728:738	ABST assays	728:738	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	40	dep	menchanism	683:692	arg1	DPPH					695:698	DPPH	695:698	DPPH	695:698	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	8	41	theme	various	1642:1648	arg1	polysaccharides					1656:1670	various plant polysaccharides	1642:1670	various plant polysaccharides	1642:1670	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	3	42	theme	ABST	728:731	arg1	assays					733:738	ABST assays	728:738	ABST assays	728:738	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	7	43	theme	degradation	1487:1497	arg1	technology					1499:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology	1414:1508	an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum	1414:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	8	44	theme	polysaccharides	1656:1670	arg1	utilization					1627:1637	utilization	1627:1637	utilization	1627:1637	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	8	44	theme	polysaccharides	1656:1670	arg1	development					1611:1621	development	1611:1621	development	1611:1621	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	1	45	theme	crude	201:205	arg1	PSP					245:247	PSP	245:247	PSP	245:247	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	45	theme	crude	201:205	arg1	polysaccharide					229:242	crude Polygonatum sibiricum polysaccharide	201:242	crude Polygonatum sibiricum polysaccharide (PSP)	201:248	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	7	46	from	sibiricum	1566:1574	arg1	polysaccharides					1533:1547	the neutral polysaccharides	1521:1547	the neutral polysaccharides from Polygonatum sibiricum	1521:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	6	47	theme	microwave-assisted	1221:1238	arg1	approach					1252:1259	the proposed microwave-assisted degradation approach	1208:1259	the proposed microwave-assisted degradation approach	1208:1259	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	6	47	theme	microwave-assisted	1221:1238	arg1	methodology					1280:1290	an efficacious methodology	1265:1290	an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides	1265:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	3	48	theme	antioxidant	589:599	arg1	activity					601:608	The antioxidant activity	585:608	The antioxidant activity of four polysaccharides fractions	585:642	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	4	49	theme	lowest	858:863	arg1	activity					877:884	the lowest antioxidant activity	854:884	the lowest antioxidant activity	854:884	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	2	50	theme	ultraviolet-visible	555:573	arg1	detector					575:582	ultraviolet-visible detector	555:582	ultraviolet-visible detector	555:582	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	3	51	theme	transition	758:767	arg1	metals					769:774	transition metals	758:774	transition metals (Fe2+ and Cu2+ chelation ability)	758:808	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	51	theme	transition	758:767	arg1	Cu2+					786:789	Cu2+	786:789	Cu2+	786:789	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	3	51	theme	transition	758:767	arg1	Fe2+					777:780	Fe2+	777:780	Fe2+	777:780	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	8	52	dep	development	1611:1621	arg1	the					1607:1609	the	1607:1609	the	1607:1609	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	3	53	theme	polysaccharides	618:632	arg1	fractions					634:642	four polysaccharides fractions	613:642	four polysaccharides fractions	613:642	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	2	54	theme	detector	542:549	arg1	scattering					531:540	evaporative light scattering detector	513:549	evaporative light scattering detector	513:549	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	0	55	from	Activity	92:99	arg1	Degradation					19:29	Microwave-Assisted Degradation	0:29	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.	0:100	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	3	56	theme	chelation	791:799	arg1	ability					801:807	chelation ability	791:807	chelation ability	791:807	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	8	57	from	kind	1677:1680	arg1	life					1714:1717	our daily life	1704:1717	our daily life	1704:1717	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	5	58	theme	105	1065:1067	arg1	×					1077:1077	×	1077:1077	×	1077:1077	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	5	59	theme	P-1	1035:1037	arg1	weight					1025:1030	the molecular weight	1011:1030	the molecular weight of P-1	1011:1037	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	7	60	dep	friendly	1433:1440	arg1	efficient					1458:1466	efficient	1458:1466	efficient	1458:1466	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	7	60	dep	friendly	1433:1440	arg1	convenient					1443:1452	convenient	1443:1452	convenient	1443:1452	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	4	61	theme	antioxidant	906:916	arg1	capacity					918:925	antioxidant capacity	906:925	antioxidant capacity	906:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	2	62	theme	gel	489:491	arg1	chromatography					493:506	high performance gel chromatography	472:506	high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector	472:582	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	1	63	dep	P-1	133:135	arg1	%					179:179	1.64%	175:179	1.64%	175:179	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	P-2					146:148	P-2	146:148	P-2	146:148	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	%					167:167	1.14%	163:167	1.14%	163:167	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	%					155:155	1.95%	151:155	1.95%	151:155	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	P-4					170:172	P-4	170:172	P-4	170:172	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	%					143:143	71.40%	138:143	71.40%	138:143	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	63	dep	P-1	133:135	arg1	P-3					158:160	P-3	158:160	P-3	158:160	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	4	64	theme	>	941:941	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	64	theme	>	941:941	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	2	65	theme	high	472:475	arg1	chromatography					493:506	high performance gel chromatography	472:506	high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector	472:582	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	1	66	theme	ethanol	282:288	arg1	extraction					270:279	water extraction	264:279	water extraction	264:279	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	66	theme	ethanol	282:288	arg1	precipitation					290:302	ethanol precipitation	282:302	ethanol precipitation	282:302	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	2	67	theme	light	525:529	arg1	scattering					531:540	evaporative light scattering detector	513:549	evaporative light scattering detector	513:549	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	6	68	theme	molecular	1334:1342	arg1	weight					1344:1349	the molecular weight	1330:1349	the molecular weight of polysaccharides	1330:1368	These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.					
30908644	5	69	theme	2.33	1072:1075	arg1	×					1077:1077	×	1077:1077	×	1077:1077	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	4	70	theme	>	947:947	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	70	theme	>	947:947	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	5	71	theme	103	1079:1081	arg1	×					1077:1077	×	1077:1077	×	1077:1077	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	1	72	theme	polysaccharide	107:120	arg1	fractions					122:130	Four polysaccharide fractions	102:130	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%)	102:180	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	1	72	theme	polysaccharide	107:120	arg1	P-1					133:135	P-1	133:135	P-1	133:135	Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography.					
30908644	0	73	theme	Polysaccharide	34:47	arg1	Degradation					19:29	Microwave-Assisted Degradation	0:29	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.	0:100	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	2	74	theme	molecular	400:408	arg1	weights					410:416	Their molecular weights	394:416	Their molecular weights	394:416	Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector.					
30908644	0	75	theme	Polygonatum	54:64	arg1	sibiricum					66:74	Polygonatum sibiricum	54:74	Polygonatum sibiricum	54:74	Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity.					
30908644	5	76	dep	×	1077:1077	arg1	to					1069:1070	to	1069:1070	to	1069:1070	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	3	77	theme	electron	665:672	arg1	menchanism					683:692	the electron transfer menchanism	661:692	the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays)	661:739	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	4	78	theme	highest	815:821	arg1	P-1					840:842	The highest content fraction P-1	811:842	The highest content fraction P-1	811:842	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	5	79	theme	molecular	1015:1023	arg1	weight					1025:1030	the molecular weight	1011:1030	the molecular weight of P-1	1011:1037	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	4	80	theme	>	935:935	arg1	ranking					895:901	the ranking	891:901	the ranking of antioxidant capacity	891:925	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	4	80	theme	>	935:935	arg1	PSP					949:951	P-4 > P-3 > P-2 > PSP	931:951	P-4 > P-3 > P-2 > PSP > P-1	931:957	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	3	81	theme	ferric	701:706	arg1	power					717:721	ferric reducing power	701:721	ferric reducing power	701:721	The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability).					
30908644	4	82	theme	fraction	831:838	arg1	P-1					840:842	The highest content fraction P-1	811:842	The highest content fraction P-1	811:842	The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1.					
30908644	8	83	theme	food	1685:1688	arg1	supplement					1690:1699	food supplement	1685:1699	food supplement	1685:1699	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	5	84	theme	degraded	1121:1128	arg1	P-1					1130:1132	degraded P-1	1121:1132	degraded P-1	1121:1132	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	5	85	dep	×	1063:1063	arg1	Da					1083:1084	105 to 2.33 × 103 Da	1065:1084	2.99 × 105 to 2.33 × 103 Da	1058:1084	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	7	86	theme	neutral	1525:1531	arg1	polysaccharides					1533:1547	the neutral polysaccharides	1521:1547	the neutral polysaccharides from Polygonatum sibiricum	1521:1574	PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum.					
30908644	5	87	theme	microwave-assisted	979:996	arg1	degradation					998:1008	microwave-assisted degradation	979:1008	microwave-assisted degradation	979:1008	After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1.					
30908644	8	88	theme	plant	1650:1654	arg1	polysaccharides					1656:1670	various plant polysaccharides	1642:1670	various plant polysaccharides	1642:1670	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30908644	8	89	theme	daily	1708:1712	arg1	life					1714:1717	our daily life	1704:1717	our daily life	1704:1717	The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.					
30326225	2	0	theme	%	597:597	arg1	yield					577:581	a extraction yield	564:581	a extraction yield of 1.10 ± 0.03%	564:597	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	1	1	theme	surface	322:328	arg1	methodology					330:340	response surface methodology	313:340	response surface methodology	313:340	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	4	2	theme	electron	1097:1104	arg1	microscopy					1106:1115	scanning electron microscopy	1088:1115	scanning electron microscopy (SEM)	1088:1121	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	2	theme	electron	1097:1104	arg1	SEM					1118:1120	SEM	1118:1120	SEM	1118:1120	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	3	3	theme	main	717:720	arg1	PPP2					755:758	PPP2	755:758	PPP2	755:758	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	3	theme	main	717:720	arg1	PPF1					749:752	PPF1	749:752	PPF1	749:752	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	3	theme	main	717:720	arg1	peaks					730:734	four main elution peaks	712:734	four main elution peaks	712:734	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	3	theme	main	717:720	arg1	PPF0					743:746	named PPF0	737:746	named PPF0	737:746	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	3	theme	main	717:720	arg1	PPF3					764:767	PPF3	764:767	PPF3	764:767	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	4	4	theme	structural	836:845	arg1	features					847:854	structural features	836:854	structural features	836:854	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	8	5	theme	20 μg/mL	1768:1775	arg1	concentration					1751:1763	the concentration	1747:1763	the concentration of 20 μg/mL	1747:1775	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	1	6	theme	polysaccharides	236:250	arg1	extraction					222:231	ultrasonic-cellulase synergistic extraction	189:231	ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	189:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	7	7	theme	resistance	1590:1599	arg1	cells					1607:1611	insulin resistance HepG2 cells	1582:1611	insulin resistance HepG2 cells with a dose-dependent relationship	1582:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	3	8	theme	named	737:741	arg1	peaks					730:734	four main elution peaks	712:734	four main elution peaks	712:734	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	8	theme	named	737:741	arg1	PPF0					743:746	named PPF0	737:746	named PPF0	737:746	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	2	9	theme	hydrolysis	355:364	arg1	temperature					366:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	9	theme	hydrolysis	355:364	arg1	50 °C					495:499	50 °C	495:499	50 °C	495:499	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	6	10	theme	molecular	1448:1456	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	1	11	theme	pineapple	257:265	arg1	PPP					275:277	PPP	275:277	PPP	275:277	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	1	11	theme	pineapple	257:265	arg1	pomace					267:272	pineapple pomace	257:272	pineapple pomace (PPP)	257:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	7	12	with	cells	1607:1611	arg1	relationship					1635:1646	a dose-dependent relationship	1618:1646	a dose-dependent relationship	1618:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	1	13	from	pomace	267:272	arg1	polysaccharides					236:250	polysaccharides	236:250	polysaccharides from pineapple pomace (PPP)	236:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	1	13	from	pomace	267:272	arg1	extraction					222:231	ultrasonic-cellulase synergistic extraction	189:231	ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	189:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	4	14	theme	polysaccharide	885:898	arg1	fractions					900:908	polysaccharide fractions	885:908	polysaccharide fractions (PPFs)	885:915	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	14	theme	polysaccharide	885:898	arg1	PPFs					911:914	PPFs	911:914	PPFs	911:914	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	6	15	with	rhamnose	1348:1355	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	2	16	theme	extraction	566:575	arg1	yield					577:581	a extraction yield	564:581	a extraction yield of 1.10 ± 0.03%	564:597	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	1	17	theme	optimal	155:161	arg1	conditions					174:183	the optimal extraction conditions	151:183	the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	151:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	5	18	theme	different	1178:1186	arg1	concentrations					1188:1201	different concentrations	1178:1201	different concentrations	1178:1201	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	0	19	from	pineapple	21:29	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from pineapple	0:29	Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities.					
30326225	5	20	theme	resistance	1237:1246	arg1	cells					1254:1258	insulin resistance HepG2 cells	1229:1258	insulin resistance HepG2 cells model	1229:1264	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	6	21	with	acid	1388:1391	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	1	22	theme	ultrasonic-cellulase	189:208	arg1	extraction					222:231	ultrasonic-cellulase synergistic extraction	189:231	ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	189:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	4	23	theme	high-performance	934:949	arg1	HPLC					974:977	HPLC	974:977	HPLC	974:977	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	23	theme	high-performance	934:949	arg1	chromatography					958:971	high-performance liquid chromatography	934:971	high-performance liquid chromatography (HPLC)	934:978	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	9	24	theme	potential	1872:1880	arg1	agent					1895:1899	a potential hypoglycemic agent	1870:1899	a potential hypoglycemic agent in biomedical and functional food	1870:1933	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	9	24	theme	potential	1872:1880	arg1	PPFs					1846:1849	PPFs	1846:1849	PPFs	1846:1849	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	5	25	theme	cells	1254:1258	arg1	model					1260:1264	insulin resistance HepG2 cells model	1229:1264	insulin resistance HepG2 cells model	1229:1264	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	0	26	theme	hypoglycemic	104:115	arg1	activities					117:126	hypoglycemic activities	104:126	hypoglycemic activities	104:126	Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities.					
30326225	6	27	with	glucose	1394:1400	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	2	28	theme	ultrasonic	434:443	arg1	power					445:449	ultrasonic power	434:449	ultrasonic power	434:449	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	7	29	theme	dose-dependent	1620:1633	arg1	relationship					1635:1646	a dose-dependent relationship	1618:1646	a dose-dependent relationship	1618:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	9	30	theme	biomedical	1904:1913	arg1	food					1930:1933	biomedical and functional food	1904:1933	biomedical and functional food	1904:1933	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	4	31	theme	UV	1018:1019	arg1	spectroscopy					1021:1032	UV spectroscopy	1018:1032	UV spectroscopy	1018:1032	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	9	32	theme	functional	1919:1928	arg1	food					1930:1933	biomedical and functional food	1904:1933	biomedical and functional food	1904:1933	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	2	33	theme	additive	459:466	arg1	quantity					468:475	the additive quantity	455:475	the additive quantity of cellulase	455:488	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	33	theme	additive	459:466	arg1	50 °C					495:499	50 °C	495:499	50 °C	495:499	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	6	34	theme	galacturonic	1375:1386	arg1	acid					1388:1391	galacturonic acid	1375:1391	galacturonic acid	1375:1391	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	7	35	theme	HepG2	1601:1605	arg1	cells					1607:1611	insulin resistance HepG2 cells	1582:1611	insulin resistance HepG2 cells with a dose-dependent relationship	1582:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	4	36	theme	permeation	985:994	arg1	chromatography					996:1009	gel permeation chromatography	981:1009	gel permeation chromatography (GPC)	981:1015	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	36	theme	permeation	985:994	arg1	GPC					1012:1014	GPC	1012:1014	GPC	1012:1014	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	6	37	theme	1.11 × 104	1480:1489	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	3	38	theme	cellulose	649:657	arg1	columns					693:699	DEAE-52 cellulose and Sephadex G-100 chromatography columns	641:699	DEAE-52 cellulose and Sephadex G-100 chromatography columns	641:699	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	4	39	theme	molecular	789:797	arg1	weight					799:804	The molecular weight	785:804	The molecular weight	785:804	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	0	40	theme	ultrasonic-cellulase	56:75	arg1	extraction					89:98	ultrasonic-cellulase synergistic extraction	56:98	ultrasonic-cellulase synergistic extraction	56:98	Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1725:1725	81.6%	1721:1725	81.6%	1721:1725	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1742:1742	84.2%	1738:1742	84.2%	1738:1742	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1694:1694	50.5%	1690:1694	50.5%	1690:1694	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1711:1711	53.6%	1707:1711	53.6%	1707:1711	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1732:1732	86.8%	1728:1732	86.8%	1728:1732	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1701:1701	82.1%	1697:1701	82.1%	1697:1701	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1718:1718	86.8%	1714:1718	86.8%	1714:1718	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	8	41	from	concentration	1751:1763	arg1	%					1687:1687	46.4%	1683:1687	46.4%	1683:1687	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	3	42	theme	chromatography	678:691	arg1	columns					693:699	DEAE-52 cellulose and Sephadex G-100 chromatography columns	641:699	DEAE-52 cellulose and Sephadex G-100 chromatography columns	641:699	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	2	43	theme	material	388:395	arg1	temperature					366:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	quantity					468:475	the additive quantity	455:475	the additive quantity of cellulase	455:488	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	ratio					379:383	ratio	379:383	ratio of material to water	379:404	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	value					410:414	pH value	407:414	pH value	407:414	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	power					445:449	ultrasonic power	434:449	ultrasonic power	434:449	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	50 °C					495:499	50 °C	495:499	50 °C	495:499	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	43	theme	material	388:395	arg1	time					428:431	hydrolysis time	417:431	hydrolysis time	417:431	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	3	44	theme	Sephadex	663:670	arg1	chromatography					678:691	Sephadex G-100 chromatography	663:691	Sephadex G-100 chromatography	663:691	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	9	45	from	agent	1895:1899	arg1	food					1930:1933	biomedical and functional food	1904:1933	biomedical and functional food	1904:1933	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	6	46	with	arabinose	1422:1430	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	2	47	theme	pH	407:408	arg1	value					410:414	pH value	407:414	pH value	407:414	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	5	48	theme	PPFs	1168:1171	arg1	activities					1154:1163	the hypoglycemic activities	1137:1163	the hypoglycemic activities of PPFs with different concentrations	1137:1201	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	6	49	with	fucose	1436:1441	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	2	50	theme	optimal	347:353	arg1	temperature					366:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	50	theme	optimal	347:353	arg1	50 °C					495:499	50 °C	495:499	50 °C	495:499	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	6	51	theme	2.22 × 104	1492:1501	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	1	52	theme	response	313:320	arg1	methodology					330:340	response surface methodology	313:340	response surface methodology	313:340	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	4	53	theme	appearance	860:869	arg1	morphology					871:880	appearance morphology	860:880	appearance morphology	860:880	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	6	54	with	galactose	1403:1411	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	8	55	theme	glucose	1653:1659	arg1	consumption					1661:1671	The glucose consumption	1649:1671	The glucose consumption	1649:1671	The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, respectively, without or with insulin.					
30326225	1	56	theme	synergistic	210:220	arg1	extraction					222:231	ultrasonic-cellulase synergistic extraction	189:231	ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	189:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	4	57	theme	monosaccharide	807:820	arg1	compositions					822:833	monosaccharide compositions	807:833	monosaccharide compositions	807:833	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	3	58	theme	elution	722:728	arg1	PPP2					755:758	PPP2	755:758	PPP2	755:758	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	58	theme	elution	722:728	arg1	PPF1					749:752	PPF1	749:752	PPF1	749:752	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	58	theme	elution	722:728	arg1	peaks					730:734	four main elution peaks	712:734	four main elution peaks	712:734	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	58	theme	elution	722:728	arg1	PPF0					743:746	named PPF0	737:746	named PPF0	737:746	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	3	58	theme	elution	722:728	arg1	PPF3					764:767	PPF3	764:767	PPF3	764:767	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	6	59	with	xylose	1414:1419	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	6	60	theme	6.71 × 104	1468:1477	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	7	61	theme	insulin	1582:1588	arg1	cells					1607:1611	insulin resistance HepG2 cells	1582:1611	insulin resistance HepG2 cells with a dose-dependent relationship	1582:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	4	62	theme	scanning	1088:1095	arg1	microscopy					1106:1115	scanning electron microscopy	1088:1115	scanning electron microscopy (SEM)	1088:1121	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	62	theme	scanning	1088:1095	arg1	SEM					1118:1120	SEM	1118:1120	SEM	1118:1120	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	63	theme	fractions	900:908	arg1	weight					799:804	The molecular weight	785:804	The molecular weight	785:804	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	63	theme	fractions	900:908	arg1	compositions					822:833	monosaccharide compositions	807:833	monosaccharide compositions	807:833	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	63	theme	fractions	900:908	arg1	features					847:854	structural features	836:854	structural features	836:854	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	63	theme	fractions	900:908	arg1	morphology					871:880	appearance morphology	860:880	appearance morphology	860:880	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	0	64	theme	synergistic	77:87	arg1	extraction					89:98	ultrasonic-cellulase synergistic extraction	56:98	ultrasonic-cellulase synergistic extraction	56:98	Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities.					
30326225	6	65	theme	5.1 × 103 Da	1507:1518	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	1	66	theme	extraction	163:172	arg1	conditions					174:183	the optimal extraction conditions	151:183	the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP)	151:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	5	67	theme	insulin	1229:1235	arg1	resistance					1237:1246	insulin resistance	1229:1246	insulin resistance HepG2 cells model	1229:1264	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	1	68	from	extraction	222:231	arg1	PPP					275:277	PPP	275:277	PPP	275:277	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	1	68	from	extraction	222:231	arg1	pomace					267:272	pineapple pomace	257:272	pineapple pomace (PPP)	257:278	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
30326225	5	69	theme	HepG2	1248:1252	arg1	cells					1254:1258	insulin resistance HepG2 cells	1229:1258	insulin resistance HepG2 cells model	1229:1264	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	4	70	dep	fourier	1035:1041	arg1	transform					1043:1051	transform	1043:1051	transform infrared spectroscopy (FT-IR)	1043:1081	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	9	71	theme	hypoglycemic	1882:1893	arg1	agent					1895:1899	a potential hypoglycemic agent	1870:1899	a potential hypoglycemic agent in biomedical and functional food	1870:1933	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	9	71	theme	hypoglycemic	1882:1893	arg1	PPFs					1846:1849	PPFs	1846:1849	PPFs	1846:1849	These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.					
30326225	4	72	theme	liquid	951:956	arg1	HPLC					974:977	HPLC	974:977	HPLC	974:977	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	72	theme	liquid	951:956	arg1	chromatography					958:971	high-performance liquid chromatography	934:971	high-performance liquid chromatography (HPLC)	934:978	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	2	73	theme	cellulase	480:488	arg1	temperature					366:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature	343:376	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	quantity					468:475	the additive quantity	455:475	the additive quantity of cellulase	455:488	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	ratio					379:383	ratio	379:383	ratio of material to water	379:404	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	value					410:414	pH value	407:414	pH value	407:414	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	power					445:449	ultrasonic power	434:449	ultrasonic power	434:449	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	50 °C					495:499	50 °C	495:499	50 °C	495:499	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	73	theme	cellulase	480:488	arg1	time					428:431	hydrolysis time	417:431	hydrolysis time	417:431	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	2	74	theme	hydrolysis	417:426	arg1	time					428:431	hydrolysis time	417:431	hydrolysis time	417:431	The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, respectively, giving a extraction yield of 1.10 ± 0.03%.					
30326225	5	75	theme	hypoglycemic	1141:1152	arg1	activities					1154:1163	the hypoglycemic activities	1137:1163	the hypoglycemic activities of PPFs with different concentrations	1137:1201	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	6	76	with	acid	1369:1372	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	0	77	theme	new	39:41	arg1	insight					43:49	new insight	39:49	new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities	39:126	Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities.					
30326225	7	78	theme	cells	1607:1611	arg1	development					1567:1577	the development	1563:1577	the development of insulin resistance HepG2 cells with a dose-dependent relationship	1563:1646	All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship.					
30326225	4	79	theme	gel	981:983	arg1	chromatography					996:1009	gel permeation chromatography	981:1009	gel permeation chromatography (GPC)	981:1015	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	79	theme	gel	981:983	arg1	GPC					1012:1014	GPC	1012:1014	GPC	1012:1014	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	6	80	theme	glucuronic	1358:1367	arg1	acid					1369:1372	glucuronic acid	1358:1372	glucuronic acid	1358:1372	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	3	81	theme	G-100	672:676	arg1	chromatography					678:691	Sephadex G-100 chromatography	663:691	Sephadex G-100 chromatography	663:691	PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatography columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained.					
30326225	5	82	with	PPFs	1168:1171	arg1	concentrations					1188:1201	different concentrations	1178:1201	different concentrations	1178:1201	Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro.					
30326225	4	83	theme	infrared	1053:1060	arg1	spectroscopy					1062:1073	infrared spectroscopy	1053:1073	infrared spectroscopy (FT-IR)	1053:1081	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	4	83	theme	infrared	1053:1060	arg1	FT-IR					1076:1080	FT-IR	1076:1080	FT-IR	1076:1080	The molecular weight, monosaccharide compositions, structural features and appearance morphology of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), UV spectroscopy, fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM).					
30326225	6	84	with	mannose	1339:1345	arg1	weight					1458:1463	molecular weight	1448:1463	molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively	1448:1532	Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with molecular weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, respectively.					
30326225	1	85	theme	Plackett-Burman	286:300	arg1	design					302:307	Plackett-Burman design	286:307	Plackett-Burman design	286:307	This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodology.					
31260959	3	0	dep	%	652:652	arg1	e.g.					637:640	e.g.	637:640	e.g.	637:640	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	4	1	theme	citral	932:937	arg1	concentration					939:951	citral concentration	932:951	citral concentration	932:951	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	1	2	theme	essential	165:173	arg1	concentrations					179:192	two different essential oil concentrations	151:192	two different essential oil concentrations	151:192	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	5	3	theme	controlled	1082:1091	arg1	release					1093:1099	a progressive and controlled release	1064:1099	a progressive and controlled release of the encapsulated citral	1064:1126	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	1	4	theme	oil	175:177	arg1	concentrations					179:192	two different essential oil concentrations	151:192	two different essential oil concentrations	151:192	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	0	5	theme	citral	98:103	arg1	activity					75:82	the antimicrobial activity	57:82	the antimicrobial activity of emulsified citral	57:103	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	0	6	from	Effect	0:5	arg1	activity					75:82	the antimicrobial activity	57:82	the antimicrobial activity of emulsified citral	57:103	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	0	7	theme	emulsified	87:96	arg1	citral					98:103	emulsified citral	87:103	emulsified citral	87:103	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	3	8	theme	2.5	658:660	arg1	%					661:661	%	661:661	%	661:661	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	4	9	theme	interfacial	957:967	arg1	composition					978:988	interfacial membrane composition	957:988	interfacial membrane composition	957:988	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	3	10	theme	additional	694:703	arg1	able					722:725	able	722:725	able	722:725	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	10	theme	additional	694:703	arg1	layer					712:716	the additional pectin layer	690:716	the additional pectin layer	690:716	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	11	theme	%	661:661	arg1	citral					663:668	2.5% citral	658:668	2.5% citral	658:668	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	4	12	theme	microcapsules	888:900	arg1	activity					863:870	the antibacterial activity	845:870	the antibacterial activity of the obtained microcapsules	845:900	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	2	13	theme	layer-by-layer	439:452	arg1	LBL					469:471	LBL	469:471	LBL	469:471	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	2	13	theme	layer-by-layer	439:452	arg1	microcapsules					454:466	layer-by-layer microcapsules	439:466	layer-by-layer microcapsules (LBL)	439:472	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	3	14	dep	concentration	793:805	arg1	whatever					777:784	whatever	777:784	whatever	777:784	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	2	15	theme	sodium	412:417	arg1	caseinate					419:427	sodium caseinate	412:427	sodium caseinate alone	412:433	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	5	16	theme	progressive	1066:1076	arg1	release					1093:1099	a progressive and controlled release	1064:1099	a progressive and controlled release of the encapsulated citral	1064:1126	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	4	17	theme	obtained	879:886	arg1	microcapsules					888:900	the obtained microcapsules	875:900	the obtained microcapsules	875:900	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	2	18	theme	emulsifier	344:353	arg1	compositions					355:366	two different emulsifier compositions	330:366	two different emulsifier compositions	330:366	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	2	19	theme	different	334:342	arg1	compositions					355:366	two different emulsifier compositions	330:366	two different emulsifier compositions	330:366	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	1	20	theme	same	257:260	arg1	amount					262:267	the same amount	253:267	the same amount of maltodextrins (20%)	253:290	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	20	theme	same	257:260	arg1	maltodextrins					272:284	maltodextrins	272:284	maltodextrins (20%)	272:290	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	20	theme	same	257:260	arg1	%					289:289	20%	287:289	20%	287:289	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	4	21	theme	antibacterial	849:861	arg1	activity					863:870	the antibacterial activity	845:870	the antibacterial activity of the obtained microcapsules	845:900	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	3	22	theme	encapsulation	521:533	arg1	efficiency					535:544	The encapsulation efficiency	517:544	The encapsulation efficiency	517:544	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	22	theme	encapsulation	521:533	arg1	higher					550:555	higher	550:555	higher	550:555	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	0	23	theme	drying	10:15	arg1	Effect					0:5	Effect	0:5	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.	0:104	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	1	24	theme	amount	262:267	arg1	presence					241:248	the presence	237:248	the presence of the same amount of maltodextrins (20%)	237:290	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	25	theme	Citral-in-water	106:120	arg1	emulsions					122:130	Citral-in-water emulsions	106:130	Citral-in-water emulsions	106:130	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	26	theme	%	208:208	arg1	concentrations					179:192	two different essential oil concentrations	151:192	two different essential oil concentrations	151:192	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	0	27	theme	membrane	33:40	arg1	composition					42:52	interfacial membrane composition	21:52	interfacial membrane composition	21:52	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	5	28	theme	citral	1034:1039	arg1	droplets					1041:1048	the citral droplets	1030:1048	the citral droplets	1030:1048	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	3	29	dep	%	595:595	arg1	e.g.					580:583	e.g.	580:583	e.g.	580:583	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	0	30	theme	interfacial	21:31	arg1	composition					42:52	interfacial membrane composition	21:52	interfacial membrane composition	21:52	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	1	31	theme	maltodextrins	272:284	arg1	amount					262:267	the same amount	253:267	the same amount of maltodextrins (20%)	253:290	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	31	theme	maltodextrins	272:284	arg1	maltodextrins					272:284	maltodextrins	272:284	maltodextrins (20%)	272:290	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	1	31	theme	maltodextrins	272:284	arg1	%					289:289	20%	287:289	20%	287:289	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	3	32	theme	2.5	601:603	arg1	%					604:604	%	604:604	%	604:604	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	2	33	theme	monolayer	369:377	arg1	microcapsules					379:391	monolayer microcapsules	369:391	monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL)	369:472	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	2	33	theme	monolayer	369:377	arg1	ML					394:395	ML	394:395	ML	394:395	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31260959	4	34	theme	membrane	969:976	arg1	composition					978:988	interfacial membrane composition	957:988	interfacial membrane composition	957:988	Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition.					
31260959	3	35	theme	pectin	705:710	arg1	able					722:725	able	722:725	able	722:725	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	35	theme	pectin	705:710	arg1	layer					712:716	the additional pectin layer	690:716	the additional pectin layer	690:716	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	36	theme	%	604:604	arg1	citral					606:611	2.5% citral	601:611	2.5% citral	601:611	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	0	37	theme	composition	42:52	arg1	Effect					0:5	Effect	0:5	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.	0:104	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	5	38	theme	citral	1121:1126	arg1	release					1093:1099	a progressive and controlled release	1064:1099	a progressive and controlled release of the encapsulated citral	1064:1126	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	3	39	theme	LBL	561:563	arg1	microcapsules					565:577	LBL microcapsules	561:577	LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral)	561:612	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	40	theme	ML	628:629	arg1	ones					631:634	ML ones	628:634	ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration	628:805	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	3	41	theme	citral	786:791	arg1	concentration					793:805	citral concentration	786:805	citral concentration	786:805	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	5	42	theme	layers	1011:1016	arg1	presence					995:1002	The presence	991:1002	The presence of two layers surrounding the citral droplets	991:1048	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	3	43	theme	spray-drying	756:767	arg1	process					769:775	the spray-drying process	752:775	the spray-drying process whatever citral concentration	752:805	The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ± 0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ± 0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration.					
31260959	0	44	theme	antimicrobial	61:73	arg1	activity					75:82	the antimicrobial activity	57:82	the antimicrobial activity of emulsified citral	57:103	Effect of drying and interfacial membrane composition on the antimicrobial activity of emulsified citral.					
31260959	5	45	theme	encapsulated	1108:1119	arg1	citral					1121:1126	the encapsulated citral	1104:1126	the encapsulated citral	1104:1126	The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.					
31260959	1	46	theme	different	155:163	arg1	concentrations					179:192	two different essential oil concentrations	151:192	two different essential oil concentrations	151:192	Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%).					
31260959	2	47	theme	sodium	488:493	arg1	caseinate					495:503	sodium caseinate	488:503	sodium caseinate	488:503	The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin.					
31715242	8	0	theme	pharmacological	1699:1713	arg1	activities					1715:1724	pharmacological activities	1699:1724	pharmacological activities	1699:1724	This study provides a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities.					
31715242	6	1	theme	IgA	1329:1331	arg1	levels					1333:1338	IgA levels	1329:1338	IgA levels in serum	1329:1347	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	6	2	dep	in	1180:1181	arg1	vivo					1183:1186	vivo	1183:1186	vivo	1183:1186	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	1	3	theme	structural	175:184	arg1	characteristics					186:200	the structural characteristics	171:200	the structural characteristics	171:200	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	8	4	theme	preliminary	1597:1607	arg1	basis					1609:1613	a preliminary basis	1595:1613	a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities	1595:1724	This study provides a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities.					
31715242	0	5	theme	polysaccharides	80:94	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	5	theme	polysaccharides	80:94	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	5	theme	polysaccharides	80:94	arg1	activity					64:71	immunomodulatory activity	47:71	immunomodulatory activity	47:71	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	3	6	theme	magnetic	724:731	arg1	resonance					733:741	nuclear magnetic resonance	716:741	nuclear magnetic resonance (NMR)	716:747	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	6	theme	magnetic	724:731	arg1	NMR					744:746	NMR	744:746	NMR	744:746	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	7	theme	chromatography-multi-angle	525:550	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	7	theme	chromatography-multi-angle	525:550	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	0	8	from	Purification	0:11	arg1	lucidum					111:117	Ganoderma lucidum	101:117	Ganoderma lucidum	101:117	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	7	9	theme	G.	1410:1411	arg1	polysaccharides					1421:1435	G. lucidum polysaccharides	1410:1435	G. lucidum polysaccharides	1410:1435	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	2	10	theme	Ganoderma	336:344	arg1	lucidum					346:352	Ganoderma lucidum	336:352	Ganoderma lucidum extracts	336:361	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	3	11	theme	atomic	659:664	arg1	AFM					684:686	AFM	684:686	AFM	684:686	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	11	theme	atomic	659:664	arg1	microscopy					672:681	atomic force microscopy	659:681	atomic force microscopy (AFM)	659:687	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	0	12	theme	Ganoderma	101:109	arg1	lucidum					111:117	Ganoderma lucidum	101:117	Ganoderma lucidum	101:117	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	13	from	activity	64:71	arg1	lucidum					111:117	Ganoderma lucidum	101:117	Ganoderma lucidum	101:117	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	4	14	theme	-α-d-Galp-	945:954	arg1	1→					956:957	→6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	924:988	1→	956:957	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	7	15	theme	advanced	1552:1559	arg1	conformation					1561:1572	advanced conformation	1552:1572	advanced conformation	1552:1572	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	4	16	theme	GLP-1	802:806	arg1	d-galactoglucan					841:855	d-galactoglucan	841:855	d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	841:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	4	16	theme	GLP-1	802:806	arg1	polysaccharide					808:821	The GLP-1 polysaccharide	798:821	The GLP-1 polysaccharide	798:821	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	6	17	theme	Immunomodulatory	1152:1167	arg1	activities					1169:1178	Immunomodulatory activities	1152:1178	Immunomodulatory activities in vivo	1152:1186	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	3	18	theme	light	558:562	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	18	theme	light	558:562	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	4	19	theme	random	873:878	arg1	conformation					887:898	a flexible random linear conformation	862:898	a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	862:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	2	20	theme	anion-exchange	438:451	arg1	column					453:458	a Q-Sepharose Fast Flow (QFF) strong anion-exchange column	401:458	a Q-Sepharose Fast Flow (QFF) strong anion-exchange column	401:458	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	7	21	theme	carbohydrate	1505:1516	arg1	composition					1518:1528	carbohydrate composition	1505:1528	carbohydrate composition	1505:1528	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	4	22	theme	-β-d-Glcp-	927:936	arg1	1→					956:957	→6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	924:988	1→	956:957	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	4	23	theme	flexible	864:871	arg1	conformation					887:898	a flexible random linear conformation	862:898	a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	862:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	2	24	theme	strong	431:436	arg1	column					453:458	a Q-Sepharose Fast Flow (QFF) strong anion-exchange column	401:458	a Q-Sepharose Fast Flow (QFF) strong anion-exchange column	401:458	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	5	25	theme	homogeneous	1026:1036	arg1	β-d-glucan					1038:1047	a relatively homogeneous β-d-glucan	1013:1047	a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation	1013:1149	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	5	25	theme	homogeneous	1026:1036	arg1	GLP-2					991:995	GLP-2	991:995	GLP-2	991:995	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	2	26	theme	gradient	366:373	arg1	precipitation					383:395	gradient ethanol precipitation	366:395	gradient ethanol precipitation	366:395	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	7	27	theme	molecular	1531:1539	arg1	weight					1541:1546	molecular weight	1531:1546	molecular weight	1531:1546	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	1	28	theme	immunomodulatory	220:235	arg1	activities					237:246	corresponding immunomodulatory activities	206:246	corresponding immunomodulatory activities	206:246	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	5	29	theme	-β-d-Glcp-	1089:1098	arg1	residues					1103:1110	→6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues	1065:1110	→6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation	1065:1149	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	4	30	theme	-β-d-Glcp-	967:976	arg1	residues					981:988	→6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	924:988	residues	981:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	7	31	theme	lucidum	1413:1419	arg1	polysaccharides					1421:1435	G. lucidum polysaccharides	1410:1435	G. lucidum polysaccharides	1410:1435	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	6	32	theme	in	1180:1181	arg1	activities					1169:1178	Immunomodulatory activities	1152:1178	Immunomodulatory activities in vivo	1152:1186	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	3	33	theme	laser	552:556	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	33	theme	laser	552:556	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	0	34	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	7	35	theme	structural	1470:1479	arg1	characteristics					1481:1495	their structural characteristics	1464:1495	their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation	1464:1572	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	7	35	theme	structural	1470:1479	arg1	composition					1518:1528	carbohydrate composition	1505:1528	carbohydrate composition	1505:1528	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	7	35	theme	structural	1470:1479	arg1	weight					1541:1546	molecular weight	1531:1546	molecular weight	1531:1546	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	7	35	theme	structural	1470:1479	arg1	conformation					1561:1572	advanced conformation	1552:1572	advanced conformation	1552:1572	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	6	36	theme	better	1221:1226	arg1	protection					1228:1237	better protection	1221:1237	better protection of the spleen and thymus	1221:1262	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	2	37	theme	lucidum	346:352	arg1	extracts					354:361	Ganoderma lucidum extracts	336:361	Ganoderma lucidum extracts	336:361	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	1	38	theme	G.	251:252	arg1	polysaccharides					262:276	G. lucidum polysaccharides	251:276	G. lucidum polysaccharides	251:276	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	4	39	theme	1→	978:979	arg1	residues					981:988	→6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	924:988	residues	981:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	3	40	theme	permeation	514:523	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	40	theme	permeation	514:523	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	7	41	theme	polysaccharides	1421:1435	arg1	activities					1396:1405	the immunomodulatory activities	1375:1405	the immunomodulatory activities of G. lucidum polysaccharides	1375:1435	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	1	42	theme	lucidum	254:260	arg1	polysaccharides					262:276	G. lucidum polysaccharides	251:276	G. lucidum polysaccharides	251:276	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	3	43	theme	monosaccharide	465:478	arg1	composition					480:490	The monosaccharide composition	461:490	The monosaccharide composition	461:490	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	4	44	with	d-galactoglucan	841:855	arg1	conformation					887:898	a flexible random linear conformation	862:898	a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	862:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	3	45	theme	nuclear	716:722	arg1	resonance					733:741	nuclear magnetic resonance	716:741	nuclear magnetic resonance (NMR)	716:747	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	45	theme	nuclear	716:722	arg1	NMR					744:746	NMR	744:746	NMR	744:746	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	1	46	theme	polysaccharides	262:276	arg1	activities					237:246	corresponding immunomodulatory activities	206:246	corresponding immunomodulatory activities	206:246	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	1	46	theme	polysaccharides	262:276	arg1	characteristics					186:200	the structural characteristics	171:200	the structural characteristics	171:200	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	6	47	from	levels	1333:1338	arg1	serum					1343:1347	serum	1343:1347	serum	1343:1347	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	3	48	theme	high-performance	493:508	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	48	theme	high-performance	493:508	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	6	49	theme	thymus	1257:1262	arg1	protection					1228:1237	better protection	1221:1237	better protection of the spleen and thymus	1221:1262	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	1	50	theme	preliminary	122:132	arg1	relationship					134:145	A preliminary relationship	120:145	A preliminary relationship	120:145	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	0	51	theme	immunomodulatory	47:62	arg1	activity					64:71	immunomodulatory activity	47:71	immunomodulatory activity	47:71	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	3	52	used	used	754:757	arg2	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	NMR					744:746	NMR	744:746	NMR	744:746	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	spectroscopy					637:648	Fourier-transform infrared spectroscopy	610:648	Fourier-transform infrared spectroscopy (FT-IR)	610:656	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	AFM					684:686	AFM	684:686	AFM	684:686	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	FT-IR					651:655	FT-IR	651:655	FT-IR	651:655	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	microscopy					672:681	atomic force microscopy	659:681	atomic force microscopy (AFM)	659:687	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	composition					480:490	The monosaccharide composition	461:490	The monosaccharide composition	461:490	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	analysis					702:709	methylation analysis	690:709	methylation analysis	690:709	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	52	used	used	754:757	arg2	resonance					733:741	nuclear magnetic resonance	716:741	nuclear magnetic resonance (NMR)	716:747	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	5	53	theme	spherical	1128:1136	arg1	conformation					1138:1149	a spherical conformation	1126:1149	a spherical conformation	1126:1149	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	5	54	theme	-β-d-Glcp-	1068:1077	arg1	residues					1103:1110	→6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues	1065:1110	→6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation	1065:1149	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	8	55	dep	structure	1668:1676	arg1	characterization					1678:1693	characterization	1678:1693	characterization	1678:1693	This study provides a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities.					
31715242	3	56	theme	force	666:670	arg1	AFM					684:686	AFM	684:686	AFM	684:686	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	56	theme	force	666:670	arg1	microscopy					672:681	atomic force microscopy	659:681	atomic force microscopy (AFM)	659:687	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	4	57	theme	→3	964:965	arg1	residues					981:988	→6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	924:988	residues	981:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	6	58	theme	spleen	1246:1251	arg1	protection					1228:1237	better protection	1221:1237	better protection of the spleen and thymus	1221:1262	Immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum.					
31715242	3	59	theme	scattering-refractive	564:584	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	59	theme	scattering-refractive	564:584	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	5	60	dep	-β-d-Glcp-	1068:1077	arg1	→3					1086:1087	→3	1086:1087	→3	1086:1087	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	5	60	dep	-β-d-Glcp-	1068:1077	arg1	1→					1079:1080	1→	1079:1080	1→	1079:1080	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	0	61	from	lucidum	111:117	arg1	polysaccharides					80:94	the polysaccharides	76:94	the polysaccharides from Ganoderma lucidum	76:117	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	61	from	lucidum	111:117	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	61	from	lucidum	111:117	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	0	61	from	lucidum	111:117	arg1	activity					64:71	immunomodulatory activity	47:71	immunomodulatory activity	47:71	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	4	62	theme	linear	880:885	arg1	conformation					887:898	a flexible random linear conformation	862:898	a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues	862:988	The GLP-1 polysaccharide was elucidated as d-galactoglucan with a flexible random linear conformation that mainly composed of →6)-β-d-Glcp-(1→, →6)-α-d-Galp-(1→, and →3)-β-d-Glcp-(1→ residues.					
31715242	2	63	theme	ethanol	375:381	arg1	precipitation					383:395	gradient ethanol precipitation	366:395	gradient ethanol precipitation	366:395	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	3	64	theme	gel	510:512	arg1	HPGPC-MALLS-RI					593:606	HPGPC-MALLS-RI	593:606	HPGPC-MALLS-RI	593:606	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	64	theme	gel	510:512	arg1	index					586:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index	493:590	high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI)	493:607	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	2	65	dep	polysaccharides	283:297	arg1	polysaccharides					283:297	Two polysaccharides	279:297	Two polysaccharides (GLP-1 and GLP-2)	279:315	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	2	65	dep	polysaccharides	283:297	arg1	GLP-2					310:314	GLP-2	310:314	GLP-2	310:314	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	2	65	dep	polysaccharides	283:297	arg1	GLP-1					300:304	GLP-1	300:304	GLP-1	300:304	Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column.					
31715242	3	66	theme	methylation	690:700	arg1	analysis					702:709	methylation analysis	690:709	methylation analysis	690:709	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	5	67	dep	β-d-glucan	1038:1047	arg1	possessing					1054:1063	possessing	1054:1063	possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation	1054:1149	GLP-2 was found to be a relatively homogeneous β-d-glucan that possessing →6)-β-d-Glcp-(1→ and →3)-β-d-Glcp-(1→ residues packaged into a spherical conformation.					
31715242	3	68	theme	Fourier-transform	610:626	arg1	FT-IR					651:655	FT-IR	651:655	FT-IR	651:655	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	68	theme	Fourier-transform	610:626	arg1	spectroscopy					637:648	Fourier-transform infrared spectroscopy	610:648	Fourier-transform infrared spectroscopy (FT-IR)	610:656	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	1	69	theme	corresponding	206:218	arg1	activities					237:246	corresponding immunomodulatory activities	206:246	corresponding immunomodulatory activities	206:246	A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides.					
31715242	0	70	from	characterization	25:40	arg1	lucidum					111:117	Ganoderma lucidum	101:117	Ganoderma lucidum	101:117	Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum.					
31715242	7	71	theme	immunomodulatory	1379:1394	arg1	activities					1396:1405	the immunomodulatory activities	1375:1405	the immunomodulatory activities of G. lucidum polysaccharides	1375:1435	Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation.					
31715242	3	72	theme	infrared	628:635	arg1	FT-IR					651:655	FT-IR	651:655	FT-IR	651:655	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	3	72	theme	infrared	628:635	arg1	spectroscopy					637:648	Fourier-transform infrared spectroscopy	610:648	Fourier-transform infrared spectroscopy (FT-IR)	610:656	The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides.					
31715242	8	73	theme	polysaccharide	1653:1666	arg1	structure					1668:1676	polysaccharide structure characterization and pharmacological activities	1653:1724	structure	1668:1676	This study provides a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities.					
31743713	6	0	theme	methylation	533:543	arg1	analysis					545:552	methylation analysis	533:552	methylation analysis	533:552	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	9	1	theme	linkage	984:990	arg1	analysis					992:999	Structural and linkage analysis	969:999	analysis	992:999	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	6	2	theme	NMR	603:605	arg1	spectroscopy					607:618	1D NMR spectroscopy	600:618	1D NMR spectroscopy	600:618	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	6	3	dep	along	555:559	arg1	with					561:564	with	561:564	with	561:564	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	0	4	from	characterization	11:26	arg1	Jujuba					85:90	Jujuba	85:90	Jujuba	85:90	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	8	5	theme	molecular	938:946	arg1	weight					948:953	an average molecular weight	927:953	an average molecular weight of 58.21 kDa	927:966	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	6	6	theme	1D	600:601	arg1	spectroscopy					607:618	1D NMR spectroscopy	600:618	1D NMR spectroscopy	600:618	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	10	7	theme	unique	1164:1169	arg1	structure					1171:1179	Such unique structure	1159:1179	Such unique structure of PZMP3-2	1159:1190	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	2	8	theme	Zizyphus	111:118	arg1	Mill					127:130	Zizyphus jujuba Mill.	111:131	Zizyphus jujuba Mill.	111:131	Jujube (Zizyphus jujuba Mill.)					
31743713	2	8	theme	Zizyphus	111:118	arg1	Jujube					103:108	Jujube	103:108	Jujube (Zizyphus jujuba Mill.)	103:132	Jujube (Zizyphus jujuba Mill.)					
31743713	8	9	theme	HPGPC	903:907	arg1	data					909:912	the HPGPC data	899:912	the HPGPC data	899:912	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	3	10	theme	health	161:166	arg1	food					168:171	a health food	159:171	a health food	159:171	have been widely used as a health food and medicinal herb in oriental medicine.					
31743713	10	11	theme	distinct	1205:1212	arg1	bioactivities					1214:1226	distinct bioactivities	1205:1226	distinct bioactivities	1205:1226	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	5	12	theme	hot	344:346	arg1	extraction					354:363	hot water extraction	344:363	hot water extraction	344:363	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	10	13	from	bioactivities	1214:1226	arg1	food					1255:1258	food	1255:1258	food	1255:1258	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	10	13	from	bioactivities	1214:1226	arg1	clinic					1269:1274	even clinic	1264:1274	even clinic	1264:1274	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	3	14	theme	oriental	195:202	arg1	medicine					204:211	oriental medicine	195:211	oriental medicine	195:211	have been widely used as a health food and medicinal herb in oriental medicine.					
31743713	9	15	theme	Rhap	1144:1147	arg1	residues					1149:1156	some Araf and Rhap residues	1130:1156	residues	1149:1156	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	5	16	theme	column	456:461	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	17	theme	water	348:352	arg1	extraction					354:363	hot water extraction	344:363	hot water extraction	344:363	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	4	18	attach	isolated	296:303	arg2	PZMP3-2					283:289	PZMP3-2	283:289	PZMP3-2	283:289	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	4	18	attach	isolated	296:303	arg1	Jujuba					319:324	Jujuba	319:324	Jujuba	319:324	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	4	18	attach	isolated	296:303	arg2	polysaccharide					267:280	a novel galacturonic acid-rich polysaccharide	236:280	a novel galacturonic acid-rich polysaccharide (PZMP3-2)	236:290	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	8	19	theme	galactose	819:827	arg1	presence					786:793	the presence	782:793	the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00	782:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	4	20	theme	acid-rich	257:265	arg1	polysaccharide					267:280	a novel galacturonic acid-rich polysaccharide	236:280	a novel galacturonic acid-rich polysaccharide (PZMP3-2)	236:290	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	4	20	theme	acid-rich	257:265	arg1	PZMP3-2					283:289	PZMP3-2	283:289	PZMP3-2	283:289	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	9	21	theme	Araf	1135:1138	arg1	residues					1149:1156	some Araf and Rhap residues	1130:1156	residues	1149:1156	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	3	22	theme	medicinal	177:185	arg1	herb					187:190	medicinal herb	177:190	medicinal herb	177:190	have been widely used as a health food and medicinal herb in oriental medicine.					
31743713	8	23	theme	molar	857:861	arg1	ratio					863:867	a molar ratio	855:867	a molar ratio of 1.74:2.00	855:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	6	24	theme	PZMP3-2	506:512	arg1	structures					492:501	The chemical structures	479:501	The chemical structures of PZMP3-2	479:512	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	8	25	theme	58.21 kDa	958:966	arg1	weight					948:953	an average molecular weight	927:953	an average molecular weight of 58.21 kDa	927:966	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	26	theme	1.74:2.00	872:880	arg1	ratio					863:867	a molar ratio	855:867	a molar ratio of 1.74:2.00	855:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	9	27	theme	NMR	1014:1016	arg1	spectroscopy					1018:1029	NMR spectroscopy	1014:1029	NMR spectroscopy	1014:1029	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	0	28	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.	0:94	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	8	29	attach	presence	786:793	arg2	acid					847:850	galacturonic acid	834:850	galacturonic acid	834:850	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	29	attach	presence	786:793	arg2	arabinose					808:816	arabinose	808:816	arabinose	808:816	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	29	attach	presence	786:793	arg2	galactose					819:827	galactose	819:827	galactose	819:827	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	29	attach	presence	786:793	arg1	ratio					863:867	a molar ratio	855:867	a molar ratio of 1.74:2.00	855:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	29	attach	presence	786:793	arg2	rhamnose					798:805	rhamnose	798:805	rhamnose	798:805	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	5	30	theme	Sephacryl	440:448	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	8	31	theme	rhamnose	798:805	arg1	presence					786:793	the presence	782:793	the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00	782:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	5	32	theme	S-300	450:454	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	10	33	from	application	1240:1250	arg1	food					1255:1258	food	1255:1258	food	1255:1258	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	10	33	from	application	1240:1250	arg1	clinic					1269:1274	even clinic	1264:1274	even clinic	1264:1274	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	6	34	theme	FT-IR	573:577	arg1	spectroscopy					579:590	FT-IR spectroscopy	573:590	FT-IR spectroscopy	573:590	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	6	35	theme	chemical	483:490	arg1	structures					492:501	The chemical structures	479:501	The chemical structures of PZMP3-2	479:512	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	8	36	theme	acid	847:850	arg1	presence					786:793	the presence	782:793	the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00	782:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	4	37	theme	galacturonic	244:255	arg1	polysaccharide					267:280	a novel galacturonic acid-rich polysaccharide	236:280	a novel galacturonic acid-rich polysaccharide (PZMP3-2)	236:290	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	4	37	theme	galacturonic	244:255	arg1	PZMP3-2					283:289	PZMP3-2	283:289	PZMP3-2	283:289	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	0	38	theme	acid-rich	46:54	arg1	polysaccharide					56:69	a galacturonic acid-rich polysaccharide	31:69	a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv	31:93	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	6	39	theme	2D NMR	625:630	arg1	spectroscopy					632:643	2D NMR spectroscopy	625:643	2D NMR spectroscopy	625:643	The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy.					
31743713	8	40	theme	galacturonic	834:845	arg1	acid					847:850	galacturonic acid	834:850	galacturonic acid	834:850	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	4	41	theme	novel	238:242	arg1	polysaccharide					267:280	a novel galacturonic acid-rich polysaccharide	236:280	a novel galacturonic acid-rich polysaccharide (PZMP3-2)	236:290	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	4	41	theme	novel	238:242	arg1	PZMP3-2					283:289	PZMP3-2	283:289	PZMP3-2	283:289	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	0	42	theme	galacturonic	33:44	arg1	polysaccharide					56:69	a galacturonic acid-rich polysaccharide	31:69	a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv	31:93	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	0	43	from	Jujuba	85:90	arg1	polysaccharide					56:69	a galacturonic acid-rich polysaccharide	31:69	a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv	31:93	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	0	43	from	Jujuba	85:90	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.	0:94	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	8	44	theme	PZMP3-2	765:771	arg1	analysis					753:760	Monosaccharide compositional analysis	724:760	Monosaccharide compositional analysis of PZMP3-2	724:771	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	9	45	theme	-like	1081:1085	arg1	polysaccharide					1094:1107	a homogalacturonan (HG)-like pectic polysaccharide	1058:1107	a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues	1058:1156	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	9	45	theme	-like	1081:1085	arg1	PZMP3-2					1046:1052	PZMP3-2	1046:1052	PZMP3-2	1046:1052	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	9	46	theme	Structural	969:978	arg1	analysis					992:999	Structural and linkage analysis	969:999	analysis	992:999	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	10	47	theme	even	1264:1267	arg1	clinic					1269:1274	even clinic	1264:1274	even clinic	1264:1274	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	9	48	theme	pectic	1087:1092	arg1	polysaccharide					1094:1107	a homogalacturonan (HG)-like pectic polysaccharide	1058:1107	a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues	1058:1156	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	9	48	theme	pectic	1087:1092	arg1	PZMP3-2					1046:1052	PZMP3-2	1046:1052	PZMP3-2	1046:1052	Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues.					
31743713	3	49	from	food	168:171	arg1	medicine					204:211	oriental medicine	195:211	oriental medicine	195:211	have been widely used as a health food and medicinal herb in oriental medicine.					
31743713	0	50	theme	polysaccharide	56:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.	0:94	Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv.					
31743713	8	51	theme	Monosaccharide	724:737	arg1	analysis					753:760	Monosaccharide compositional analysis	724:760	Monosaccharide compositional analysis of PZMP3-2	724:771	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	2	52	theme	jujuba	120:125	arg1	Mill					127:130	Zizyphus jujuba Mill.	111:131	Zizyphus jujuba Mill.	111:131	Jujube (Zizyphus jujuba Mill.)					
31743713	2	52	theme	jujuba	120:125	arg1	Jujube					103:108	Jujube	103:108	Jujube (Zizyphus jujuba Mill.)	103:132	Jujube (Zizyphus jujuba Mill.)					
31743713	7	53	theme	morphological	650:662	arg1	properties					664:673	Its morphological properties	646:673	Its morphological properties	646:673	Its morphological properties were further characterized by SEM, AFM, and XRD.					
31743713	5	54	theme	DEAE-Sepharose	411:424	arg1	Flow					431:434	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	Flow	431:434	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	8	55	theme	average	930:936	arg1	weight					948:953	an average molecular weight	927:953	an average molecular weight of 58.21 kDa	927:966	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	5	56	theme	Fast	426:429	arg1	Flow					431:434	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	Flow	431:434	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	57	dep	Muzao	330:334	arg1	purification					393:404	purification	393:404	purification	393:404	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	57	dep	Muzao	330:334	arg1	extraction					354:363	hot water extraction	344:363	hot water extraction	344:363	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	57	dep	Muzao	330:334	arg1	precipitation					374:386	ethanol precipitation	366:386	ethanol precipitation	366:386	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	3	58	from	herb	187:190	arg1	medicine					204:211	oriental medicine	195:211	oriental medicine	195:211	have been widely used as a health food and medicinal herb in oriental medicine.					
31743713	10	59	theme	PZMP3-2	1184:1190	arg1	structure					1171:1179	Such unique structure	1159:1179	Such unique structure of PZMP3-2	1159:1190	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	8	60	theme	compositional	739:751	arg1	analysis					753:760	Monosaccharide compositional analysis	724:760	Monosaccharide compositional analysis of PZMP3-2	724:771	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	8	61	theme	arabinose	808:816	arg1	presence					786:793	the presence	782:793	the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00	782:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	5	62	with	precipitation	374:386	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	62	with	precipitation	374:386	arg1	Flow					431:434	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	Flow	431:434	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	4	63	theme	present	221:227	arg1	study					229:233	the present study	217:233	the present study	217:233	In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv.					
31743713	5	64	theme	ethanol	366:372	arg1	precipitation					374:386	ethanol precipitation	366:386	ethanol precipitation	366:386	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	10	65	theme	Such	1159:1162	arg1	structure					1171:1179	Such unique structure	1159:1179	Such unique structure of PZMP3-2	1159:1190	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31743713	5	66	with	purification	393:404	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	66	with	purification	393:404	arg1	Flow					431:434	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	Flow	431:434	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	8	67	from	ratio	863:867	arg1	presence					786:793	the presence	782:793	the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00	782:880	Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa.					
31743713	5	68	with	extraction	354:363	arg1	chromatography					463:476	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	chromatography	463:476	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	5	68	with	extraction	354:363	arg1	Flow					431:434	DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography	411:476	Flow	431:434	Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography.					
31743713	10	69	theme	further	1232:1238	arg1	application					1240:1250	further application	1232:1250	further application in food and even clinic	1232:1274	Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.					
31430160	10	0	dep	sialylation	1621:1631	arg1	metastasis					1657:1666	brain metastasis	1651:1666	brain metastasis	1651:1666	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	7	1	theme	efficient	1071:1079	arg1	separation					1090:1099	an efficient isomeric separation	1068:1099	an efficient isomeric separation	1068:1099	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	9	2	theme	cancer	1417:1422	arg1	invasion					1424:1431	breast cancer invasion	1410:1431	breast cancer invasion	1410:1431	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	8	3	theme	expression	1220:1229	arg1	alterations					1231:1241	significant expression alterations	1208:1241	significant expression alterations of these glycan isomers	1208:1265	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	13	4	from	expression	2107:2116	arg1	231BR					2145:2149	brain-seeking cell line 231BR	2121:2149	brain-seeking cell line 231BR	2121:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	5	theme	N-glycan	761:768	arg1	isomers					770:776	N-glycan isomers	761:776	N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line	761:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	12	6	link	α2,6-linked	1943:1953	arg1	acids					1962:1966	α2,6-linked sialic acids	1943:1966	α2,6-linked sialic acids	1943:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	6	7	theme	cell	842:845	arg1	line					847:850	one brain cancer cell line	825:850	one brain cancer cell line	825:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	0	8	theme	Chromatography-Tandem	133:153	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	8	theme	Chromatography-Tandem	133:153	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	7	9	theme	temperature	1030:1040	arg1	nanoPGC-LC-MS/MS					1042:1057	The high temperature nanoPGC-LC-MS/MS	1021:1057	The high temperature nanoPGC-LC-MS/MS	1021:1057	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	1	10	theme	Breast	185:190	arg1	cancer					212:217	a leading cancer	202:217	a leading cancer in women	202:226	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	10	theme	Breast	185:190	arg1	cancer					286:291	the second-most common metastatic cancer	252:291	the second-most common metastatic cancer following lung cancer	252:313	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	10	theme	Breast	185:190	arg1	cancer					192:197	Breast cancer	185:197	Breast cancer	185:197	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	12	11	theme	cancer	2008:2013	arg1	metastasis					2015:2024	breast cancer metastasis	2001:2024	breast cancer metastasis	2001:2024	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	8	12	theme	glycan	1252:1257	arg1	isomers					1259:1265	these glycan isomers	1246:1265	these glycan isomers	1246:1265	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	5	13	theme	breast	683:688	arg1	metastasis					703:712	breast cancer brain metastasis	683:712	breast cancer brain metastasis	683:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	11	14	theme	breast	1842:1847	arg1	metastasis					1862:1871	breast cancer brain metastasis	1842:1871	breast cancer brain metastasis	1842:1871	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	5	15	theme	brain	697:701	arg1	metastasis					703:712	breast cancer brain metastasis	683:712	breast cancer brain metastasis	683:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	5	16	from	metastasis	703:712	arg1	studies					644:650	studies	644:650	studies of N-glycan isomer function in breast cancer brain metastasis	644:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	1	17	theme	leading	204:210	arg1	cancer					212:217	a leading cancer	202:217	a leading cancer in women	202:226	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	17	theme	leading	204:210	arg1	cancer					192:197	Breast cancer	185:197	Breast cancer	185:197	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	18	from	cancer	212:217	arg1	women					222:226	women	222:226	women	222:226	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	8	19	theme	different	1277:1285	arg1	lines					1306:1310	the different breast cancer cell lines	1273:1310	the different breast cancer cell lines	1273:1310	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	10	20	theme	glycan	1614:1619	arg1	sialylation					1621:1631	glycan sialylation	1614:1631	glycan sialylation	1614:1631	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	5	21	theme	N-glycan	655:662	arg1	function					671:678	N-glycan isomer function	655:678	N-glycan isomer function in breast cancer brain metastasis	655:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	13	22	theme	cell	2135:2138	arg1	231BR					2145:2149	brain-seeking cell line 231BR	2121:2149	brain-seeking cell line 231BR	2121:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	23	theme	brain-seeking	888:900	arg1	line					907:910	a brain-seeking cell line	886:910	a brain-seeking cell line	886:910	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	23	theme	brain-seeking	888:900	arg1	231BR					913:917	231BR	913:917	231BR	913:917	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	13	24	dep	roles	2212:2216	arg1	play					2233:2236	play	2233:2236	play during breast cancer metastasis	2233:2268	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	8	25	theme	cancer	1294:1299	arg1	lines					1306:1310	the different breast cancer cell lines	1273:1310	the different breast cancer cell lines	1273:1310	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	5	26	theme	function	671:678	arg1	studies					644:650	studies	644:650	studies of N-glycan isomer function in breast cancer brain metastasis	644:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	6	27	theme	glycan	966:971	arg1	isomers					973:979	glycan isomers	966:979	glycan isomers	966:979	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	0	28	theme	Breast	59:64	arg1	Metastasis					79:88	Breast Cancer Brain Metastasis	59:88	Breast Cancer Brain Metastasis	59:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	13	29	theme	isomeric	2098:2105	arg1	expression					2107:2116	the unique isomeric expression	2087:2116	the unique isomeric expression in brain-seeking cell line 231BR	2087:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	13	29	theme	isomeric	2098:2105	arg1	expressions					2063:2073	glycan isomer expressions	2049:2073	glycan isomer expressions	2049:2073	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	10	30	dep	isomers	1460:1466	arg1	regard					1439:1444	regard	1439:1444	regard	1439:1444	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	9	31	theme	total	1329:1333	arg1	abundance					1342:1350	total glycan abundance	1329:1350	total glycan abundance	1329:1350	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	12	32	gly	sialylated	1912:1921	arg1	isomers					1930:1936	highly sialylated glycan isomers	1905:1936	highly sialylated glycan isomers with α2,6-linked sialic acids	1905:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	11	33	theme	cells	1785:1789	arg1	passage					1760:1766	the passage	1756:1766	the passage of breast cancer cells through the blood-brain barrier	1756:1821	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	0	34	theme	Brain	73:77	arg1	Metastasis					79:88	Breast Cancer Brain Metastasis	59:88	Breast Cancer Brain Metastasis	59:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	7	35	from	identification	1119:1132	arg1	compositions					1183:1194	50 N-glycan compositions	1171:1194	50 N-glycan compositions	1171:1194	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	11	36	theme	breast	1771:1776	arg1	cells					1785:1789	breast cancer cells	1771:1789	breast cancer cells	1771:1789	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	7	37	theme	N-glycan	1174:1181	arg1	compositions					1183:1194	50 N-glycan compositions	1171:1194	50 N-glycan compositions	1171:1194	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	12	38	theme	sialic	1955:1960	arg1	acids					1962:1966	α2,6-linked sialic acids	1943:1966	α2,6-linked sialic acids	1943:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	7	39	from	quantitation	1138:1149	arg1	compositions					1183:1194	50 N-glycan compositions	1171:1194	50 N-glycan compositions	1171:1194	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	0	40	theme	Graphitic	103:111	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	40	theme	Graphitic	103:111	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	9	41	theme	glycan	1335:1340	arg1	abundance					1342:1350	total glycan abundance	1329:1350	total glycan abundance	1329:1350	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	10	42	theme	individual	1449:1458	arg1	isomers					1460:1466	individual isomers	1449:1466	individual isomers	1449:1466	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	10	43	theme	brain	1651:1655	arg1	metastasis					1657:1666	brain metastasis	1651:1666	brain metastasis	1651:1666	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	13	44	theme	more	2274:2277	arg1	clues					2279:2283	more clues	2274:2283	more clues for a deeper insight of this bioprocess	2274:2323	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	12	45	theme	glycan	1923:1928	arg1	isomers					1930:1936	highly sialylated glycan isomers	1905:1936	highly sialylated glycan isomers with α2,6-linked sialic acids	1905:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	6	46	theme	brain	1003:1007	arg1	metastasis					1009:1018	breast cancer brain metastasis	989:1018	breast cancer brain metastasis	989:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	2	47	theme	diagnostic	407:416	arg1	cases					418:422	the diagnostic cases	403:422	the diagnostic cases of breast cancer brain metastasis	403:456	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	0	48	theme	Biological	14:23	arg1	Attributes					25:34	the Biological Attributes	10:34	the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis	10:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	1	49	theme	common	268:273	arg1	cancer					192:197	Breast cancer	185:197	Breast cancer	185:197	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	49	theme	common	268:273	arg1	cancer					286:291	the second-most common metastatic cancer	252:291	the second-most common metastatic cancer following lung cancer	252:313	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	6	50	theme	cancer	835:840	arg1	line					847:850	one brain cancer cell line	825:850	one brain cancer cell line	825:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	51	theme	breast	989:994	arg1	metastasis					1009:1018	breast cancer brain metastasis	989:1018	breast cancer brain metastasis	989:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	2	52	theme	metastasis	447:456	arg1	cases					418:422	the diagnostic cases	403:422	the diagnostic cases of breast cancer brain metastasis	403:456	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	4	53	theme	Aberrant	542:549	arg1	glycosylation					551:563	Aberrant glycosylation	542:563	Aberrant glycosylation	542:563	Aberrant glycosylation has been proved to be related to many diseases and cancer metastasis.					
31430160	10	54	theme	greatest	1473:1480	arg1	number					1482:1487	the greatest number	1469:1487	the greatest number of sialylated isomers	1469:1509	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	11	55	theme	level	1719:1723	arg1	increase					1686:1693	the increase	1682:1693	the increase of the α2,6-sialylation level in 231BR	1682:1732	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	9	56	theme	abundance	1342:1350	arg1	increase					1317:1324	The increase	1313:1324	The increase of total glycan abundance and sialylation level	1313:1372	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	2	57	theme	cancer	434:439	arg1	metastasis					447:456	breast cancer brain metastasis	427:456	breast cancer brain metastasis	427:456	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	12	58	with	isomers	1930:1936	arg1	acids					1962:1966	α2,6-linked sialic acids	1943:1966	α2,6-linked sialic acids	1943:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	13	59	theme	glycan	2049:2054	arg1	expression					2107:2116	the unique isomeric expression	2087:2116	the unique isomeric expression in brain-seeking cell line 231BR	2087:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	13	59	theme	glycan	2049:2054	arg1	expressions					2063:2073	glycan isomer expressions	2049:2073	glycan isomer expressions	2049:2073	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	2	60	theme	10-16	329:333	arg1	patients					353:360	breast cancer patients	339:360	breast cancer patients	339:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	2	60	theme	10-16	329:333	arg1	%					334:334	An estimated 10-16%	316:334	An estimated 10-16% of breast cancer patients	316:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	0	61	theme	Isomers	48:54	arg1	Attributes					25:34	the Biological Attributes	10:34	the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis	10:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	9	62	theme	sialylation	1356:1366	arg1	level					1368:1372	sialylation level	1356:1372	sialylation level	1356:1372	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	10	63	theme	isomers	1503:1509	arg1	number					1482:1487	the greatest number	1469:1487	the greatest number of sialylated isomers	1469:1509	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	13	64	theme	expressions	2063:2073	arg1	investigation					2032:2044	This investigation	2027:2044	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR,	2027:2150	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	11	65	theme	brain	1856:1860	arg1	metastasis					1862:1871	breast cancer brain metastasis	1842:1871	breast cancer brain metastasis	1842:1871	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	13	66	theme	glycan	2218:2223	arg1	isomers					2225:2231	glycan isomers	2218:2231	glycan isomers	2218:2231	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	67	theme	cancer	803:808	arg1	lines					815:819	five breast cancer cell lines	791:819	five breast cancer cell lines	791:819	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	10	68	theme	expression	1547:1556	arg1	alterations					1558:1568	significant expression alterations	1535:1568	significant expression alterations in 231BR	1535:1577	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	2	69	theme	cancer	346:351	arg1	patients					353:360	breast cancer patients	339:360	breast cancer patients	339:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	8	70	theme	significant	1208:1218	arg1	alterations					1231:1241	significant expression alterations	1208:1241	significant expression alterations of these glycan isomers	1208:1265	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	13	71	theme	cancer	2252:2257	arg1	metastasis					2259:2268	breast cancer metastasis	2245:2268	breast cancer metastasis	2245:2268	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	7	72	theme	isomeric	1081:1088	arg1	separation					1090:1099	an efficient isomeric separation	1068:1099	an efficient isomeric separation	1068:1099	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	9	73	theme	breast	1410:1415	arg1	invasion					1424:1431	breast cancer invasion	1410:1431	breast cancer invasion	1410:1431	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	6	74	theme	isomers	770:776	arg1	expressions					746:756	the expressions	742:756	the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line	742:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	75	attach	derived	778:784	arg1	lines					815:819	five breast cancer cell lines	791:819	five breast cancer cell lines	791:819	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	75	attach	derived	778:784	arg2	isomers					770:776	N-glycan isomers	761:776	N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line	761:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	75	attach	derived	778:784	arg1	line					847:850	one brain cancer cell line	825:850	one brain cancer cell line	825:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	10	76	gly	sialylated	1492:1501	arg1	isomers					1503:1509	sialylated isomers	1492:1509	sialylated isomers	1492:1509	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	1	77	theme	lung	303:306	arg1	cancer					308:313	lung cancer	303:313	lung cancer	303:313	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	7	78	theme	high	1025:1028	arg1	nanoPGC-LC-MS/MS					1042:1057	The high temperature nanoPGC-LC-MS/MS	1021:1057	The high temperature nanoPGC-LC-MS/MS	1021:1057	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	0	79	theme	PGC	121:123	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	79	theme	PGC	121:123	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	12	80	theme	breast	2001:2006	arg1	metastasis					2015:2024	breast cancer metastasis	2001:2024	breast cancer metastasis	2001:2024	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	10	81	theme	breast	1637:1642	arg1	cancer					1644:1649	breast cancer	1637:1649	breast cancer	1637:1649	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	11	82	theme	blood-brain	1803:1813	arg1	barrier					1815:1821	the blood-brain barrier	1799:1821	the blood-brain barrier	1799:1821	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	5	83	theme	cancer	690:695	arg1	metastasis					703:712	breast cancer brain metastasis	683:712	breast cancer brain metastasis	683:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	13	84	theme	new	2161:2163	arg1	information					2165:2175	new information	2161:2175	new information toward understanding the potential roles glycan isomers play during breast cancer metastasis	2161:2268	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	4	85	theme	cancer	616:621	arg1	metastasis					623:632	cancer metastasis	616:632	cancer metastasis	616:632	Aberrant glycosylation has been proved to be related to many diseases and cancer metastasis.					
31430160	11	86	theme	cancer	1849:1854	arg1	metastasis					1862:1871	breast cancer brain metastasis	1842:1871	breast cancer brain metastasis	1842:1871	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	0	87	theme	Mass	155:158	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	87	theme	Mass	155:158	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	13	88	theme	bioprocess	2314:2323	arg1	insight					2298:2304	a deeper insight	2289:2304	a deeper insight of this bioprocess	2289:2323	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	89	theme	cell	902:905	arg1	line					907:910	a brain-seeking cell line	886:910	a brain-seeking cell line	886:910	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	89	theme	cell	902:905	arg1	231BR					913:917	231BR	913:917	231BR	913:917	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	7	90	from	compositions	1183:1194	arg1	quantitation					1138:1149	quantitation	1138:1149	quantitation	1138:1149	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	7	90	from	compositions	1183:1194	arg1	isomers					1158:1164	144 isomers	1154:1164	144 isomers from 50 N-glycan compositions	1154:1194	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	7	90	from	compositions	1183:1194	arg1	identification					1119:1132	identification	1119:1132	identification	1119:1132	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	10	91	from	alterations	1558:1568	arg1	231BR					1573:1577	231BR	1573:1577	231BR	1573:1577	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	5	92	theme	isomer	664:669	arg1	function					671:678	N-glycan isomer function	655:678	N-glycan isomer function in breast cancer brain metastasis	655:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	13	93	theme	line	2140:2143	arg1	231BR					2145:2149	brain-seeking cell line 231BR	2121:2149	brain-seeking cell line 231BR	2121:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	8	94	theme	breast	1287:1292	arg1	lines					1306:1310	the different breast cancer cell lines	1273:1310	the different breast cancer cell lines	1273:1310	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	0	95	theme	Cancer	66:71	arg1	Metastasis					79:88	Breast Cancer Brain Metastasis	59:88	Breast Cancer Brain Metastasis	59:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	13	96	theme	deeper	2291:2296	arg1	insight					2298:2304	a deeper insight	2289:2304	a deeper insight of this bioprocess	2289:2323	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	97	theme	role	961:964	arg1	understanding					940:952	a better understanding	931:952	a better understanding of the role glycan isomers play in breast cancer brain metastasis	931:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	0	98	from	Attributes	25:34	arg1	Metastasis					79:88	Breast Cancer Brain Metastasis	59:88	Breast Cancer Brain Metastasis	59:88	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	8	99	theme	cell	1301:1304	arg1	lines					1306:1310	the different breast cancer cell lines	1273:1310	the different breast cancer cell lines	1273:1310	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	12	100	theme	α2,6-linked	1943:1953	arg1	acids					1962:1966	α2,6-linked sialic acids	1943:1966	α2,6-linked sialic acids	1943:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	0	101	theme	Porous	96:101	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	101	theme	Porous	96:101	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	13	102	theme	brain-seeking	2121:2133	arg1	231BR					2145:2149	brain-seeking cell line 231BR	2121:2149	brain-seeking cell line 231BR	2121:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	6	103	theme	better	933:938	arg1	understanding					940:952	a better understanding	931:952	a better understanding of the role glycan isomers play in breast cancer brain metastasis	931:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	6	104	dep	role	961:964	arg1	play					981:984	play	981:984	play in breast cancer brain metastasis	981:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	11	105	theme	cancer	1778:1783	arg1	cells					1785:1789	breast cancer cells	1771:1789	breast cancer cells	1771:1789	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	7	106	theme	isomers	1158:1164	arg1	quantitation					1138:1149	quantitation	1138:1149	quantitation	1138:1149	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	7	106	theme	isomers	1158:1164	arg1	identification					1119:1132	identification	1119:1132	identification	1119:1132	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	12	107	theme	sialylated	1912:1921	arg1	isomers					1930:1936	highly sialylated glycan isomers	1905:1936	highly sialylated glycan isomers with α2,6-linked sialic acids	1905:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	0	108	theme	Carbon	113:118	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	108	theme	Carbon	113:118	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	13	109	theme	unique	2091:2096	arg1	expression					2107:2116	the unique isomeric expression	2087:2116	the unique isomeric expression in brain-seeking cell line 231BR	2087:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	13	109	theme	unique	2091:2096	arg1	expressions					2063:2073	glycan isomer expressions	2049:2073	glycan isomer expressions	2049:2073	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	12	110	theme	isomers	1930:1936	arg1	Meanwhile					1874:1882	Meanwhile	1874:1882	Meanwhile	1874:1882	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	12	110	theme	isomers	1930:1936	arg1	upregulation					1889:1900	the upregulation	1885:1900	the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids	1885:1966	Meanwhile, the upregulation of highly sialylated glycan isomers with α2,6-linked sialic acids were found to be associated with breast cancer metastasis.					
31430160	0	111	theme	Liquid	126:131	arg1	LC-MS/MS					174:181	LC-MS/MS	174:181	LC-MS/MS	174:181	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	0	111	theme	Liquid	126:131	arg1	Spectrometry					160:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry	96:171	Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	96:182	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	7	112	dep	identification	1119:1132	arg1	the					1115:1117	the	1115:1117	the	1115:1117	The high temperature nanoPGC-LC-MS/MS achieved an efficient isomeric separation and permitted the identification and quantitation of 144 isomers from 50 N-glycan compositions.					
31430160	2	113	theme	patients	353:360	arg1	patients					353:360	breast cancer patients	339:360	breast cancer patients	339:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	2	113	theme	patients	353:360	arg1	%					334:334	An estimated 10-16%	316:334	An estimated 10-16% of breast cancer patients	316:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	1	114	theme	second-most	256:266	arg1	cancer					192:197	Breast cancer	185:197	Breast cancer	185:197	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	114	theme	second-most	256:266	arg1	cancer					286:291	the second-most common metastatic cancer	252:291	the second-most common metastatic cancer following lung cancer	252:313	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	6	115	theme	cancer	996:1001	arg1	metastasis					1009:1018	breast cancer brain metastasis	989:1018	breast cancer brain metastasis	989:1018	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	11	116	from	increase	1686:1693	arg1	231BR					1728:1732	231BR	1728:1732	231BR	1728:1732	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	11	117	theme	α2,6-sialylation	1702:1717	arg1	level					1719:1723	the α2,6-sialylation level	1698:1723	the α2,6-sialylation level	1698:1723	Furthermore, the increase of the α2,6-sialylation level in 231BR likely contributes to the passage of breast cancer cells through the blood-brain barrier, thus facilitating breast cancer brain metastasis.					
31430160	0	118	theme	N-Glycan	39:46	arg1	Isomers					48:54	N-Glycan Isomers	39:54	N-Glycan Isomers	39:54	Revealing the Biological Attributes of N-Glycan Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon (PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).					
31430160	1	119	theme	metastatic	275:284	arg1	cancer					192:197	Breast cancer	185:197	Breast cancer	185:197	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	1	119	theme	metastatic	275:284	arg1	cancer					286:291	the second-most common metastatic cancer	252:291	the second-most common metastatic cancer following lung cancer	252:313	Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer.					
31430160	6	120	theme	brain	829:833	arg1	line					847:850	one brain cancer cell line	825:850	one brain cancer cell line	825:850	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	2	121	theme	brain	441:445	arg1	metastasis					447:456	breast cancer brain metastasis	427:456	breast cancer brain metastasis	427:456	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	2	122	theme	brain	381:385	arg1	metastasis					387:396	brain metastasis	381:396	brain metastasis	381:396	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	5	123	from	studies	644:650	arg1	metastasis					703:712	breast cancer brain metastasis	683:712	breast cancer brain metastasis	683:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	2	124	theme	breast	427:432	arg1	metastasis					447:456	breast cancer brain metastasis	427:456	breast cancer brain metastasis	427:456	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	13	125	theme	isomer	2056:2061	arg1	expression					2107:2116	the unique isomeric expression	2087:2116	the unique isomeric expression in brain-seeking cell line 231BR	2087:2149	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	13	125	theme	isomer	2056:2061	arg1	expressions					2063:2073	glycan isomer expressions	2049:2073	glycan isomer expressions	2049:2073	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	2	126	theme	estimated	319:327	arg1	patients					353:360	breast cancer patients	339:360	breast cancer patients	339:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	2	126	theme	estimated	319:327	arg1	%					334:334	An estimated 10-16%	316:334	An estimated 10-16% of breast cancer patients	316:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31430160	9	127	theme	level	1368:1372	arg1	increase					1317:1324	The increase	1313:1324	The increase of total glycan abundance and sialylation level	1313:1372	The increase of total glycan abundance and sialylation level were observed to be associated with breast cancer invasion.					
31430160	6	128	theme	cell	810:813	arg1	lines					815:819	five breast cancer cell lines	791:819	five breast cancer cell lines	791:819	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	8	129	theme	isomers	1259:1265	arg1	alterations					1231:1241	significant expression alterations	1208:1241	significant expression alterations of these glycan isomers	1208:1265	There were significant expression alterations of these glycan isomers among the different breast cancer cell lines.					
31430160	10	130	theme	sialylated	1492:1501	arg1	isomers					1503:1509	sialylated isomers	1492:1509	sialylated isomers	1492:1509	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	13	131	theme	breast	2245:2250	arg1	metastasis					2259:2268	breast cancer metastasis	2245:2268	breast cancer metastasis	2245:2268	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	5	132	from	function	671:678	arg1	metastasis					703:712	breast cancer brain metastasis	683:712	breast cancer brain metastasis	683:712	However, studies of N-glycan isomer function in breast cancer brain metastasis are limited.					
31430160	6	133	theme	breast	796:801	arg1	lines					815:819	five breast cancer cell lines	791:819	five breast cancer cell lines	791:819	In this study, the expressions of N-glycan isomers derived from five breast cancer cell lines and one brain cancer cell line were investigated and compared to a brain-seeking cell line, 231BR, to acquire a better understanding of the role glycan isomers play in breast cancer brain metastasis.					
31430160	13	134	theme	potential	2202:2210	arg1	roles					2212:2216	the potential roles	2198:2216	the potential roles glycan isomers play during breast cancer metastasis	2198:2268	This investigation of glycan isomer expressions, especially the unique isomeric expression in brain-seeking cell line 231BR, provides new information toward understanding the potential roles glycan isomers play during breast cancer metastasis and more clues for a deeper insight of this bioprocess.					
31430160	4	135	theme	many	598:601	arg1	diseases					603:610	many diseases	598:610	many diseases	598:610	Aberrant glycosylation has been proved to be related to many diseases and cancer metastasis.					
31430160	10	136	theme	significant	1535:1545	arg1	alterations					1558:1568	significant expression alterations	1535:1568	significant expression alterations in 231BR	1535:1577	With regard to individual isomers, the greatest number of sialylated isomers was observed along with significant expression alterations in 231BR, suggesting a relationship between glycan sialylation and breast cancer brain metastasis.					
31430160	2	137	theme	breast	339:344	arg1	patients					353:360	breast cancer patients	339:360	breast cancer patients	339:360	An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing.					
31121231	0	0	theme	Panax	88:92	arg1	marc					102:105	Panax ginseng marc	88:105	Panax ginseng marc	88:105	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	2	1	theme	functional	338:347	arg1	food					349:352	functional food	338:352	functional food with health-promoting properties	338:385	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	2	2	from	polysaccharides	270:284	arg1	rich					252:255	rich	252:255	rich	252:255	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	5	3	theme	important	894:902	arg1	which					884:888	which	884:888	which	884:888	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	3	theme	important	894:902	arg1	regulators					911:920	important immune regulators	894:920	important immune regulators of T-cells	894:931	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	1	4	theme	ginseng	150:156	arg1	industry					158:165	the ginseng industry	146:165	the ginseng industry currently used as animal feed or fertilizer	146:209	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	4	5	theme	polysaccharides	581:595	arg1	structure					544:552	structure	544:552	structure	544:552	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	4	5	theme	polysaccharides	581:595	arg1	activity					569:576	biological activity	558:576	biological activity	558:576	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	4	5	theme	polysaccharides	581:595	arg1	composition					531:541	composition	531:541	composition	531:541	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	5	6	theme	root	731:734	arg1	marc					736:739	root marc	731:739	root marc in high abundance	731:757	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	2	7	theme	wall	265:268	arg1	polysaccharides					270:284	cell wall polysaccharides	260:284	cell wall polysaccharides	260:284	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	5	8	theme	infection	967:975	arg1	processes					977:985	infection processes	967:985	infection processes	967:985	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	4	9	theme	root	610:613	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	0	10	theme	ginseng	94:100	arg1	marc					102:105	Panax ginseng marc	88:105	Panax ginseng marc	88:105	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	2	11	theme	cell	260:263	arg1	polysaccharides					270:284	cell wall polysaccharides	260:284	cell wall polysaccharides	260:284	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	1	12	theme	major	126:130	arg1	marc					116:119	Ginseng marc	108:119	Ginseng marc	108:119	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	1	12	theme	major	126:130	arg1	by-product					132:141	a major by-product	124:141	a major by-product of the ginseng industry currently used as animal feed or fertilizer	124:209	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	1	13	theme	industry	158:165	arg1	marc					116:119	Ginseng marc	108:119	Ginseng marc	108:119	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	1	13	theme	industry	158:165	arg1	by-product					132:141	a major by-product	124:141	a major by-product of the ginseng industry currently used as animal feed or fertilizer	124:209	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	4	14	theme	ginseng	602:608	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	5	15	theme	immune	904:909	arg1	which					884:888	which	884:888	which	884:888	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	15	theme	immune	904:909	arg1	regulators					911:920	important immune regulators	894:920	important immune regulators of T-cells	894:931	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	4	16	from	activity	569:576	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	6	17	theme	higher	1107:1112	arg1	value					1114:1118	a higher value	1105:1118	a higher value than animal feed	1105:1135	Our study reveals the potential to increase the value of ginseng marc by generating carbohydrate-based products with a higher value than animal feed.					
31121231	5	18	theme	macrophage	866:875	arg1	cells					877:881	RAW 264.7 murine macrophage cells	849:881	RAW 264.7 murine macrophage cells	849:881	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	6	19	theme	ginseng	1045:1051	arg1	marc					1053:1056	ginseng marc	1045:1056	ginseng marc	1045:1056	Our study reveals the potential to increase the value of ginseng marc by generating carbohydrate-based products with a higher value than animal feed.					
31121231	5	20	theme	inflammatory	940:951	arg1	responses					953:961	inflammatory responses	940:961	inflammatory responses	940:961	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	0	21	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	4	22	theme	biological	558:567	arg1	activity					569:576	biological activity	558:576	biological activity	558:576	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	2	23	theme	health-promoting	359:374	arg1	properties					376:385	health-promoting properties	359:385	health-promoting properties	359:385	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	0	24	theme	biological	24:33	arg1	activity					35:42	biological activity	24:42	biological activity	24:42	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	4	25	from	composition	531:541	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	5	26	theme	high	744:747	arg1	abundance					749:757	high abundance	744:757	high abundance	744:757	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	1	27	theme	animal	185:190	arg1	feed					192:195	animal feed	185:195	animal feed	185:195	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	5	28	theme	growth	832:837	arg1	factor					839:844	the hematopoietic growth factor	814:844	the hematopoietic growth factor	814:844	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	29	dep	activate	767:774	arg1	which					884:888	which	884:888	which	884:888	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	29	dep	activate	767:774	arg1	regulators					911:920	important immune regulators	894:920	important immune regulators of T-cells	894:931	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	1	30	theme	Ginseng	108:114	arg1	marc					116:119	Ginseng marc	108:119	Ginseng marc	108:119	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	1	30	theme	Ginseng	108:114	arg1	by-product					132:141	a major by-product	124:141	a major by-product of the ginseng industry currently used as animal feed or fertilizer	124:209	Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer.					
31121231	3	31	theme	exact	470:474	arg1	composition					476:486	their exact composition	464:486	their exact composition	464:486	However, the extraction of these polysaccharides has proved problematic and their exact composition remains unknown.					
31121231	4	32	from	structure	544:552	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	6	33	theme	marc	1053:1056	arg1	value					1036:1040	the value	1032:1040	the value of ginseng marc	1032:1056	Our study reveals the potential to increase the value of ginseng marc by generating carbohydrate-based products with a higher value than animal feed.					
31121231	3	34	theme	polysaccharides	421:435	arg1	extraction					401:410	the extraction	397:410	the extraction of these polysaccharides	397:435	However, the extraction of these polysaccharides has proved problematic and their exact composition remains unknown.					
31121231	5	35	theme	RAW	849:851	arg1	cells					877:881	RAW 264.7 murine macrophage cells	849:881	RAW 264.7 murine macrophage cells	849:881	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	36	theme	murine	859:864	arg1	cells					877:881	RAW 264.7 murine macrophage cells	849:881	RAW 264.7 murine macrophage cells	849:881	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	2	37	theme	fibrous	217:223	arg1	stream					242:247	This fibrous, insoluble waste stream	212:247	This fibrous, insoluble waste stream	212:247	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	2	38	dep	fibrous	217:223	arg1	insoluble					226:234	insoluble	226:234	insoluble	226:234	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	0	39	theme	wall	52:55	arg1	polysaccharides					57:71	cell wall polysaccharides	47:71	cell wall polysaccharides	47:71	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	5	40	theme	interleukine-1α	794:808	arg1	production					780:789	the production	776:789	the production of interleukine-1α and the hematopoietic growth factor	776:844	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	41	from	marc	736:739	arg1	abundance					749:757	high abundance	744:757	high abundance	744:757	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	2	42	theme	ingredients	322:332	arg1	source					312:317	a potential source	300:317	a potential source of ingredients for functional food with health-promoting properties	300:385	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	0	43	theme	cell	47:50	arg1	polysaccharides					57:71	cell wall polysaccharides	47:71	cell wall polysaccharides	47:71	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	5	44	theme	hematopoietic	818:830	arg1	factor					839:844	the hematopoietic growth factor	814:844	the hematopoietic growth factor	814:844	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	4	45	theme	alkali	669:674	arg1	solutions					676:684	alkali solutions	669:684	alkali solutions	669:684	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	5	46	theme	pectic	691:696	arg1	fraction					698:705	The pectic fraction	687:705	The pectic fraction	687:705	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	47	theme	T-cells	925:931	arg1	which					884:888	which	884:888	which	884:888	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	5	47	theme	T-cells	925:931	arg1	regulators					911:920	important immune regulators	894:920	important immune regulators of T-cells	894:931	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	2	48	from	rich	252:255	arg1	polysaccharides					270:284	cell wall polysaccharides	260:284	cell wall polysaccharides	260:284	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	4	49	theme	stem	616:619	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	6	50	theme	carbohydrate-based	1072:1089	arg1	products					1091:1098	carbohydrate-based products	1072:1098	carbohydrate-based products	1072:1098	Our study reveals the potential to increase the value of ginseng marc by generating carbohydrate-based products with a higher value than animal feed.					
31121231	2	51	theme	potential	302:310	arg1	source					312:317	a potential source	300:317	a potential source of ingredients for functional food with health-promoting properties	300:385	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	2	52	with	food	349:352	arg1	properties					376:385	health-promoting properties	359:385	health-promoting properties	359:385	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	2	53	theme	waste	236:240	arg1	stream					242:247	This fibrous, insoluble waste stream	212:247	This fibrous, insoluble waste stream	212:247	This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties.					
31121231	5	54	theme	factor	839:844	arg1	production					780:789	the production	776:789	the production of interleukine-1α and the hematopoietic growth factor	776:844	The pectic fraction has been extracted from root marc in high abundance and can activate the production of interleukine-1α and the hematopoietic growth factor by RAW 264.7 murine macrophage cells, which are important immune regulators of T-cells during inflammatory responses and infection processes.					
31121231	6	55	theme	animal	1125:1130	arg1	feed					1132:1135	animal feed	1125:1135	animal feed	1125:1135	Our study reveals the potential to increase the value of ginseng marc by generating carbohydrate-based products with a higher value than animal feed.					
31121231	4	56	theme	leaf	625:628	arg1	marc					630:633	ginseng root, stem and leaf marc	602:633	marc	630:633	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	0	57	theme	polysaccharides	57:71	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	0	57	theme	polysaccharides	57:71	arg1	activity					35:42	biological activity	24:42	biological activity	24:42	Structural analysis and biological activity of cell wall polysaccharides extracted from Panax ginseng marc.					
31121231	4	58	from	marc	630:633	arg1	polysaccharides					581:595	polysaccharides	581:595	polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions	581:684	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	4	58	from	marc	630:633	arg1	structure					544:552	structure	544:552	structure	544:552	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	4	58	from	marc	630:633	arg1	activity					569:576	biological activity	558:576	biological activity	558:576	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31121231	4	58	from	marc	630:633	arg1	composition					531:541	composition	531:541	composition	531:541	Here we have analysed the composition, structure and biological activity of polysaccharides from ginseng root, stem and leaf marc fractionated using a chelator and alkali solutions.					
31151515	8	0	theme	apparent	1078:1085	arg1	viscosity					1087:1095	higher apparent viscosity	1071:1095	higher apparent viscosity	1071:1095	WSF and SSF showed higher apparent viscosity and elasticity.					
31151515	7	1	theme	thermal	1033:1039	arg1	stability					1041:1049	higher thermal stability	1026:1049	higher thermal stability	1026:1049	Thermal analysis manifested that LKF and TKF exhibited higher thermal stability.					
31151515	4	2	with	aggregative	710:720	arg1	weight					779:784	extremely high molecular weight	754:784	extremely high molecular weight	754:784	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	6	3	theme	FTIR	955:958	arg1	spectrums					960:968	special FTIR spectrums	947:968	special FTIR spectrums	947:968	These polysaccharides exhibited distinct surface morphology and special FTIR spectrums.					
31151515	8	4	theme	higher	1071:1076	arg1	viscosity					1087:1095	higher apparent viscosity	1071:1095	higher apparent viscosity	1071:1095	WSF and SSF showed higher apparent viscosity and elasticity.					
31151515	1	5	theme	tomato	353:358	arg1	polysaccharides					370:384	tomato cell wall polysaccharides	353:384	tomato cell wall polysaccharides	353:384	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	4	6	theme	molecular	769:777	arg1	weight					779:784	extremely high molecular weight	754:784	extremely high molecular weight	754:784	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	5	7	from	rich	800:803	arg1	sugars					816:821	neutral sugars	808:821	neutral sugars	808:821	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	0	8	theme	tomato	114:119	arg1	fruit					121:125	tomato fruit	114:125	tomato fruit	114:125	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	1	9	theme	cell	360:363	arg1	polysaccharides					370:384	tomato cell wall polysaccharides	353:384	tomato cell wall polysaccharides	353:384	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	9	10	theme	antioxidant	1195:1205	arg1	activities					1207:1216	stronger antioxidant activities	1186:1216	stronger antioxidant activities	1186:1216	Assays for functional bioactivities suggested that CSF and SSF displayed stronger antioxidant activities, while CSF, SSF and TKF exhibited higher hypolipidemic activities.					
31151515	3	11	theme	pectic	567:572	arg1	CSF					536:538	CSF	536:538	CSF	536:538	WSF, CSF and SSF were identified as pectic polysaccharides, while LKF and TKF were identified as hemicellulose.					
31151515	3	11	theme	pectic	567:572	arg1	WSF					531:533	WSF	531:533	WSF	531:533	WSF, CSF and SSF were identified as pectic polysaccharides, while LKF and TKF were identified as hemicellulose.					
31151515	3	11	theme	pectic	567:572	arg1	polysaccharides					574:588	pectic polysaccharides	567:588	pectic polysaccharides	567:588	WSF, CSF and SSF were identified as pectic polysaccharides, while LKF and TKF were identified as hemicellulose.					
31151515	3	11	theme	pectic	567:572	arg1	SSF					544:546	SSF	544:546	SSF	544:546	WSF, CSF and SSF were identified as pectic polysaccharides, while LKF and TKF were identified as hemicellulose.					
31151515	9	12	theme	functional	1124:1133	arg1	bioactivities					1135:1147	functional bioactivities	1124:1147	functional bioactivities	1124:1147	Assays for functional bioactivities suggested that CSF and SSF displayed stronger antioxidant activities, while CSF, SSF and TKF exhibited higher hypolipidemic activities.					
31151515	1	13	theme	wall	365:368	arg1	polysaccharides					370:384	tomato cell wall polysaccharides	353:384	tomato cell wall polysaccharides	353:384	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	9	14	theme	higher	1252:1257	arg1	activities					1273:1282	higher hypolipidemic activities	1252:1282	higher hypolipidemic activities	1252:1282	Assays for functional bioactivities suggested that CSF and SSF displayed stronger antioxidant activities, while CSF, SSF and TKF exhibited higher hypolipidemic activities.					
31151515	2	15	theme	functional	418:427	arg1	bioactivities					429:441	functional bioactivities	418:441	functional bioactivities	418:441	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	0	16	theme	different	59:67	arg1	polysaccharides					83:97	different bonding state polysaccharides	59:97	different bonding state polysaccharides	59:97	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	5	17	from	sugars	816:821	arg1	rich					800:803	rich	800:803	rich	800:803	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	9	18	theme	hypolipidemic	1259:1271	arg1	activities					1273:1282	higher hypolipidemic activities	1252:1282	higher hypolipidemic activities	1252:1282	Assays for functional bioactivities suggested that CSF and SSF displayed stronger antioxidant activities, while CSF, SSF and TKF exhibited higher hypolipidemic activities.					
31151515	0	19	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	4	20	theme	galacturonic	669:680	arg1	acids					682:686	galacturonic acids	669:686	galacturonic acids	669:686	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	2	21	theme	fruit	483:487	arg1	DBTP					506:509	DBTP	506:509	DBTP	506:509	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	21	theme	fruit	483:487	arg1	polysaccharides					489:503	these different bonding state tomato fruit polysaccharides	446:503	these different bonding state tomato fruit polysaccharides (DBTP)	446:510	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	6	22	theme	distinct	915:922	arg1	morphology					932:941	distinct surface morphology	915:941	distinct surface morphology	915:941	These polysaccharides exhibited distinct surface morphology and special FTIR spectrums.					
31151515	4	23	theme	high	764:767	arg1	weight					779:784	extremely high molecular weight	754:784	extremely high molecular weight	754:784	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	2	24	theme	tomato	476:481	arg1	DBTP					506:509	DBTP	506:509	DBTP	506:509	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	24	theme	tomato	476:481	arg1	polysaccharides					489:503	these different bonding state tomato fruit polysaccharides	446:503	these different bonding state tomato fruit polysaccharides (DBTP)	446:510	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	7	25	theme	Thermal	971:977	arg1	analysis					979:986	Thermal analysis	971:986	Thermal analysis	971:986	Thermal analysis manifested that LKF and TKF exhibited higher thermal stability.					
31151515	5	26	theme	side	823:826	arg1	CSF					787:789	CSF	787:789	CSF	787:789	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	5	26	theme	side	823:826	arg1	chains					828:833	rich in neutral sugars side chains	800:833	rich in neutral sugars side chains	800:833	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	1	27	theme	sodium	187:192	arg1	fraction					212:219	sodium carbonate-soluble fraction	187:219	sodium carbonate-soluble fraction (SSF)	187:225	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	27	theme	sodium	187:192	arg1	SSF					222:224	SSF	222:224	SSF	222:224	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	0	28	theme	functional	31:40	arg1	bioactivities					42:54	functional bioactivities	31:54	functional bioactivities	31:54	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	1	29	theme	tightly-bonding	276:290	arg1	TKF					315:317	TKF	315:317	TKF	315:317	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	29	theme	tightly-bonding	276:290	arg1	fractions					304:312	tightly-bonding KOH-soluble fractions	276:312	tightly-bonding KOH-soluble fractions (TKF)	276:318	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	5	30	contain	contained	836:844	arg1	CSF					787:789	CSF	787:789	CSF	787:789	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	5	30	contain	contained	836:844	arg1	chains					828:833	rich in neutral sugars side chains	800:833	rich in neutral sugars side chains	800:833	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	5	30	contain	contained	836:844	arg2	regions					874:880	abundant rhamnogalacturonan regions	846:880	abundant rhamnogalacturonan regions	846:880	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	5	30	contain	contained	836:844	arg1	SSF					795:797	SSF	795:797	SSF	795:797	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	1	31	theme	carbonate-soluble	194:210	arg1	fraction					212:219	sodium carbonate-soluble fraction	187:219	sodium carbonate-soluble fraction (SSF)	187:225	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	31	theme	carbonate-soluble	194:210	arg1	SSF					222:224	SSF	222:224	SSF	222:224	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	2	32	theme	bonding	462:468	arg1	DBTP					506:509	DBTP	506:509	DBTP	506:509	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	32	theme	bonding	462:468	arg1	polysaccharides					489:503	these different bonding state tomato fruit polysaccharides	446:503	these different bonding state tomato fruit polysaccharides (DBTP)	446:510	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	1	33	theme	KOH-soluble	292:302	arg1	TKF					315:317	TKF	315:317	TKF	315:317	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	33	theme	KOH-soluble	292:302	arg1	fractions					304:312	tightly-bonding KOH-soluble fractions	276:312	tightly-bonding KOH-soluble fractions (TKF)	276:318	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	34	theme	Water-soluble	128:140	arg1	WSF					152:154	WSF	152:154	WSF	152:154	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	34	theme	Water-soluble	128:140	arg1	fraction					142:149	Water-soluble fraction	128:149	Water-soluble fraction (WSF)	128:155	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	5	35	theme	abundant	846:853	arg1	regions					874:880	abundant rhamnogalacturonan regions	846:880	abundant rhamnogalacturonan regions	846:880	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	2	36	theme	Physicochemical	387:401	arg1	properties					403:412	Physicochemical properties	387:412	Physicochemical properties	387:412	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	5	37	theme	rhamnogalacturonan	855:872	arg1	regions					874:880	abundant rhamnogalacturonan regions	846:880	abundant rhamnogalacturonan regions	846:880	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	4	38	theme	acids	682:686	arg1	plenty					659:664	plenty	659:664	plenty of galacturonic acids	659:686	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	5	39	theme	rich	800:803	arg1	CSF					787:789	CSF	787:789	CSF	787:789	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	5	39	theme	rich	800:803	arg1	chains					828:833	rich in neutral sugars side chains	800:833	rich in neutral sugars side chains	800:833	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	2	40	theme	state	470:474	arg1	DBTP					506:509	DBTP	506:509	DBTP	506:509	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	40	theme	state	470:474	arg1	polysaccharides					489:503	these different bonding state tomato fruit polysaccharides	446:503	these different bonding state tomato fruit polysaccharides (DBTP)	446:510	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	9	41	theme	stronger	1186:1193	arg1	activities					1207:1216	stronger antioxidant activities	1186:1216	stronger antioxidant activities	1186:1216	Assays for functional bioactivities suggested that CSF and SSF displayed stronger antioxidant activities, while CSF, SSF and TKF exhibited higher hypolipidemic activities.					
31151515	6	42	theme	special	947:953	arg1	spectrums					960:968	special FTIR spectrums	947:968	special FTIR spectrums	947:968	These polysaccharides exhibited distinct surface morphology and special FTIR spectrums.					
31151515	0	43	theme	bonding	69:75	arg1	polysaccharides					83:97	different bonding state polysaccharides	59:97	different bonding state polysaccharides	59:97	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	5	44	theme	neutral	808:814	arg1	sugars					816:821	neutral sugars	808:821	neutral sugars	808:821	CSF and SSF, rich in neutral sugars side chains, contained abundant rhamnogalacturonan regions.					
31151515	4	45	contain	possessing	648:657	arg2	plenty					659:664	plenty	659:664	plenty of galacturonic acids	659:686	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	4	45	contain	possessing	648:657	arg1	aggregative					710:720	aggregative	710:720	aggregative	710:720	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	4	45	contain	possessing	648:657	arg1	WSF					643:645	WSF	643:645	WSF	643:645	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	6	46	theme	surface	924:930	arg1	morphology					932:941	distinct surface morphology	915:941	distinct surface morphology	915:941	These polysaccharides exhibited distinct surface morphology and special FTIR spectrums.					
31151515	4	47	theme	linear	725:730	arg1	homogalacturonan					732:747	linear homogalacturonan	725:747	linear homogalacturonan	725:747	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	2	48	theme	polysaccharides	489:503	arg1	properties					403:412	Physicochemical properties	387:412	Physicochemical properties	387:412	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	48	theme	polysaccharides	489:503	arg1	bioactivities					429:441	functional bioactivities	418:441	functional bioactivities	418:441	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	4	49	theme	homogalacturonan	732:747	arg1	aggregative					710:720	aggregative	710:720	aggregative	710:720	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	4	49	theme	homogalacturonan	732:747	arg1	WSF					643:645	WSF	643:645	WSF	643:645	WSF, possessing plenty of galacturonic acids, was considered as an aggregative of linear homogalacturonan with extremely high molecular weight.					
31151515	0	50	theme	polysaccharides	83:97	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	0	50	theme	polysaccharides	83:97	arg1	bioactivities					42:54	functional bioactivities	31:54	functional bioactivities	31:54	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	2	51	theme	different	452:460	arg1	DBTP					506:509	DBTP	506:509	DBTP	506:509	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	2	51	theme	different	452:460	arg1	polysaccharides					489:503	these different bonding state tomato fruit polysaccharides	446:503	these different bonding state tomato fruit polysaccharides (DBTP)	446:510	Physicochemical properties and functional bioactivities of these different bonding state tomato fruit polysaccharides (DBTP) were investigated.					
31151515	1	52	theme	loosely-bonding	228:242	arg1	LKF					267:269	LKF	267:269	LKF	267:269	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	52	theme	loosely-bonding	228:242	arg1	fractions					256:264	loosely-bonding KOH-soluble fractions	228:264	loosely-bonding KOH-soluble fractions (LKF)	228:270	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	0	53	theme	state	77:81	arg1	polysaccharides					83:97	different bonding state polysaccharides	59:97	different bonding state polysaccharides	59:97	Physicochemical properties and functional bioactivities of different bonding state polysaccharides extracted from tomato fruit.					
31151515	7	54	theme	higher	1026:1031	arg1	stability					1041:1049	higher thermal stability	1026:1049	higher thermal stability	1026:1049	Thermal analysis manifested that LKF and TKF exhibited higher thermal stability.					
31151515	1	55	theme	CDTA-soluble	158:169	arg1	CSF					181:183	CSF	181:183	CSF	181:183	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	55	theme	CDTA-soluble	158:169	arg1	fraction					171:178	CDTA-soluble fraction	158:178	CDTA-soluble fraction (CSF)	158:184	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	56	theme	KOH-soluble	244:254	arg1	LKF					267:269	LKF	267:269	LKF	267:269	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31151515	1	56	theme	KOH-soluble	244:254	arg1	fractions					256:264	loosely-bonding KOH-soluble fractions	228:264	loosely-bonding KOH-soluble fractions (LKF)	228:270	Water-soluble fraction (WSF), CDTA-soluble fraction (CSF), sodium carbonate-soluble fraction (SSF), loosely-bonding KOH-soluble fractions (LKF) and tightly-bonding KOH-soluble fractions (TKF) were sequentially extracted from tomato cell wall polysaccharides.					
31134626	0	0	theme	macrophage	77:86	arg1	cells					88:92	murine macrophage cells	70:92	murine macrophage cells	70:92	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	9	1	theme	antioxidant	1347:1357	arg1	potential					1359:1367	noticeable antioxidant potential	1336:1367	noticeable antioxidant potential	1336:1367	Besides, MLHWP displayed noticeable antioxidant potential as reflected in all investigating assays.					
31134626	2	2	theme	edible	352:357	arg1	mushroom					364:371	an edible wild mushroom Macrocybe lobayensis (R. Heim)	349:402	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	0	3	theme	murine	70:75	arg1	cells					88:92	murine macrophage cells	70:92	murine macrophage cells	70:92	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	6	4	theme	helical	870:876	arg1	structure					878:886	triple helical structure	863:886	triple helical structure	863:886	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	1	5	theme	complete	165:172	arg1	package					174:180	a complete package	163:180	a complete package of various medicinal activities	163:212	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	5	6	theme	ion	765:767	arg1	chelating					769:777	metal ion chelating	759:777	metal ion chelating	759:777	Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect.					
31134626	5	7	theme	antioxidant	696:706	arg1	activity					708:715	antioxidant activity	696:715	antioxidant activity	696:715	Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect.					
31134626	3	8	theme	infrared	524:531	arg1	spectroscopy					533:544	Fourier-transform infrared spectroscopy	506:544	Fourier-transform infrared spectroscopy	506:544	METHOD Chemical characterization was accomplished with the help of spectrophotometry, Fourier-transform infrared spectroscopy, HPTLC and GC-MS.					
31134626	3	9	dep	the	475:477	arg1	help					479:482	help	479:482	help	479:482	METHOD Chemical characterization was accomplished with the help of spectrophotometry, Fourier-transform infrared spectroscopy, HPTLC and GC-MS.					
31134626	4	10	theme	signalling	626:635	arg1	mechanism					637:645	its signalling mechanism	622:645	its signalling mechanism	622:645	Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells.					
31134626	7	11	theme	viability	1083:1091	arg1	terms					1074:1078	terms	1074:1078	terms of viability, phagocytosis, NO and ROS generation	1074:1128	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	7	12	theme	bioactive	1003:1011	arg1	potency					1013:1019	bioactive potency	1003:1019	bioactive potency	1003:1019	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	2	13	theme	biomedical	281:290	arg1	properties					292:301	structural and biomedical properties	266:301	structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	266:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	6	14	link	β-linked	918:925	arg1	glucan					927:932	β-linked glucan	918:932	β-linked glucan	918:932	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	4	15	theme	RAW	666:668	arg1	cells					676:680	RAW 264.7 cells	666:680	RAW 264.7 cells	666:680	Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells.					
31134626	2	16	dep	mushroom	364:371	arg1	Macrocybe					373:381	Macrocybe	373:381	Macrocybe	373:381	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	2	16	dep	mushroom	364:371	arg1	Heim					398:401	Heim	398:401	Heim	398:401	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	3	17	theme	METHOD	420:425	arg1	characterization					436:451	METHOD Chemical characterization	420:451	METHOD Chemical characterization	420:451	METHOD Chemical characterization was accomplished with the help of spectrophotometry, Fourier-transform infrared spectroscopy, HPTLC and GC-MS.					
31134626	0	18	theme	TLR/NF-κB	97:105	arg1	pathway					107:113	TLR/NF-κB pathway	97:113	TLR/NF-κB pathway	97:113	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	0	19	from	mushroom	33:40	arg1	polysaccharide					6:19	Crude polysaccharide	0:19	Crude polysaccharide from a wild mushroom	0:40	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	10	20	theme	multidimensional	1504:1519	arg1	application					1521:1531	multidimensional application	1504:1531	multidimensional application	1504:1531	CONCLUSIONS Overall, the results portrayed possibility of MLHWP as pharmaceutical agent with multidimensional application.					
31134626	2	21	theme	structural	266:275	arg1	properties					292:301	structural and biomedical properties	266:301	structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	266:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	3	22	theme	Chemical	427:434	arg1	characterization					436:451	METHOD Chemical characterization	420:451	METHOD Chemical characterization	420:451	METHOD Chemical characterization was accomplished with the help of spectrophotometry, Fourier-transform infrared spectroscopy, HPTLC and GC-MS.					
31134626	10	23	with	agent	1493:1497	arg1	application					1521:1531	multidimensional application	1504:1531	multidimensional application	1504:1531	CONCLUSIONS Overall, the results portrayed possibility of MLHWP as pharmaceutical agent with multidimensional application.					
31134626	9	24	theme	investigating	1389:1401	arg1	assays					1403:1408	all investigating assays	1385:1408	all investigating assays	1385:1408	Besides, MLHWP displayed noticeable antioxidant potential as reflected in all investigating assays.					
31134626	8	25	theme	expression	1136:1145	arg1	studies					1147:1153	Gene expression studies	1131:1153	Gene expression studies	1131:1153	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	1	26	theme	various	185:191	arg1	activities					203:212	various medicinal activities	185:212	various medicinal activities	185:212	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	0	27	theme	Crude	0:4	arg1	polysaccharide					6:19	Crude polysaccharide	0:19	Crude polysaccharide from a wild mushroom	0:40	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	4	28	theme	Immunomodulatory	564:579	arg1	crude polysaccharide					597:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells.					
31134626	2	29	theme	polysaccharide	312:325	arg1	properties					292:301	structural and biomedical properties	266:301	structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	266:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	1	30	theme	medicinal	193:201	arg1	activities					203:212	various medicinal activities	185:212	various medicinal activities	185:212	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	8	31	theme	genes	1251:1255	arg1	expression					1212:1221	expression	1212:1221	expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα	1212:1308	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	5	32	theme	radical	739:745	arg1	scavenging					747:756	radical scavenging	739:756	radical scavenging	739:756	Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect.					
31134626	6	33	theme	Compositional	813:825	arg1	study					827:831	KEY FINDINGS Compositional study	800:831	KEY FINDINGS Compositional study	800:831	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	2	34	theme	crude	306:310	arg1	MLHWP					328:332	MLHWP	328:332	MLHWP	328:332	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	2	34	theme	crude	306:310	arg1	polysaccharide					312:325	crude polysaccharide	306:325	crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	306:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	2	35	theme	&	411:411	arg1	Lodge					413:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	4	36	theme	of the	590:595	arg1	crude polysaccharide					597:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells.					
31134626	8	37	theme	Gene	1131:1134	arg1	studies					1147:1153	Gene expression studies	1131:1153	Gene expression studies	1131:1153	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	3	38	theme	Fourier-transform	506:522	arg1	spectroscopy					533:544	Fourier-transform infrared spectroscopy	506:544	Fourier-transform infrared spectroscopy	506:544	METHOD Chemical characterization was accomplished with the help of spectrophotometry, Fourier-transform infrared spectroscopy, HPTLC and GC-MS.					
31134626	2	39	theme	Pegler	404:409	arg1	Lodge					413:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	4	40	theme	activity	581:588	arg1	crude polysaccharide					597:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide	564:616	Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells.					
31134626	6	41	theme	β-linked	918:925	arg1	glucan					927:932	β-linked glucan	918:932	β-linked glucan	918:932	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	2	42	theme	present	236:242	arg1	study					244:248	the present study	232:248	the present study	232:248	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	0	43	theme	wild	28:31	arg1	mushroom					33:40	a wild mushroom	26:40	a wild mushroom	26:40	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	5	44	theme	metal	759:763	arg1	chelating					769:777	metal ion chelating	759:777	metal ion chelating	759:777	Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect.					
31134626	6	45	theme	FINDINGS	804:811	arg1	study					827:831	KEY FINDINGS Compositional study	800:831	KEY FINDINGS Compositional study	800:831	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	5	46	theme	reducing	783:790	arg1	effect					792:797	reducing effect	783:797	reducing effect	783:797	Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect.					
31134626	0	47	theme	immune	51:56	arg1	response					58:65	immune response	51:65	immune response in murine macrophage cells	51:92	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	7	48	theme	generation	1119:1128	arg1	terms					1074:1078	terms	1074:1078	terms of viability, phagocytosis, NO and ROS generation	1074:1128	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	0	49	from	response	58:65	arg1	cells					88:92	murine macrophage cells	70:92	murine macrophage cells	70:92	Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway.					
31134626	6	50	theme	KEY	800:802	arg1	FINDINGS					804:811	KEY FINDINGS	800:811	KEY FINDINGS Compositional study	800:831	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	7	51	theme	ROS	1115:1117	arg1	generation					1119:1128	ROS generation	1115:1128	ROS generation	1115:1128	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	10	52	theme	MLHWP	1469:1473	arg1	possibility					1454:1464	possibility	1454:1464	possibility of MLHWP	1454:1473	CONCLUSIONS Overall, the results portrayed possibility of MLHWP as pharmaceutical agent with multidimensional application.					
31134626	7	53	theme	NO	1108:1109	arg1	terms					1074:1078	terms	1074:1078	terms of viability, phagocytosis, NO and ROS generation	1074:1128	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	1	54	theme	OBJECTIVE	116:124	arg1	polysaccharides					141:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	10	55	theme	pharmaceutical	1478:1491	arg1	agent					1493:1497	pharmaceutical agent	1478:1497	pharmaceutical agent with multidimensional application	1478:1531	CONCLUSIONS Overall, the results portrayed possibility of MLHWP as pharmaceutical agent with multidimensional application.					
31134626	6	56	theme	triple	863:868	arg1	structure					878:886	triple helical structure	863:886	triple helical structure	863:886	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	1	57	theme	Mushroom	126:133	arg1	polysaccharides					141:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	COX-2					1274:1278	COX-2	1274:1278	COX-2	1274:1278	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	iNOS					1295:1298	iNOS	1295:1298	iNOS	1295:1298	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	Iκ-βα					1304:1308	Iκ-βα	1304:1308	Iκ-βα	1304:1308	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	genes					1251:1255	immunomodulation-related genes	1226:1255	immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα	1226:1308	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	IFN-γ					1281:1285	IFN-γ	1281:1285	IFN-γ	1281:1285	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	TNF-α					1288:1292	TNF-α	1288:1292	TNF-α	1288:1292	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	8	58	theme	immunomodulation-related	1226:1249	arg1	NF-κB					1267:1271	NF-κB	1267:1271	NF-κB	1267:1271	Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation-related genes including NF-κB, COX-2, IFN-γ, TNF-α, iNOS and Iκ-βα.					
31134626	7	59	theme	phagocytosis	1094:1105	arg1	terms					1074:1078	terms	1074:1078	terms of viability, phagocytosis, NO and ROS generation	1074:1128	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	2	60	dep	Macrocybe	373:381	arg1	lobayensis					383:392	Macrocybe lobayensis	373:392	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	7	61	theme	macrophage	1051:1060	arg1	activity					1062:1069	macrophage activity	1051:1069	macrophage activity	1051:1069	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	1	62	theme	crude	135:139	arg1	polysaccharides					141:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides	116:155	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	7	63	from	Investigation	986:998	arg1	potency					1013:1019	bioactive potency	1003:1019	bioactive potency	1003:1019	Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation.					
31134626	1	64	theme	activities	203:212	arg1	package					174:180	a complete package	163:180	a complete package of various medicinal activities	163:212	OBJECTIVE Mushroom crude polysaccharides offer a complete package of various medicinal activities.					
31134626	2	65	theme	mushroom	364:371	arg1	Lodge					413:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
31134626	6	66	contain	possessed	853:861	arg2	structure					878:886	triple helical structure	863:886	triple helical structure	863:886	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	6	66	contain	possessed	853:861	arg2	backbone					896:903	its backbone	892:903	its backbone consisted of β-linked glucan	892:932	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	6	66	contain	possessed	853:861	arg1	MLHWP					847:851	MLHWP	847:851	MLHWP	847:851	KEY FINDINGS Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β-linked glucan along with xylose, rhamnose, mannose and galactose.					
31134626	9	67	theme	noticeable	1336:1345	arg1	potential					1359:1367	noticeable antioxidant potential	1336:1367	noticeable antioxidant potential	1336:1367	Besides, MLHWP displayed noticeable antioxidant potential as reflected in all investigating assays.					
31134626	2	68	theme	wild	359:362	arg1	mushroom					364:371	an edible wild mushroom Macrocybe lobayensis (R. Heim)	349:402	an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge	349:417	In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge.					
30898558	1	0	from	sequences	410:418	arg1	family					427:432	the family	423:432	the family	423:432	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	1	1	theme	fungal	340:345	arg1	endoglucanases					347:360	GH7 fungal endoglucanases	336:360	GH7 fungal endoglucanases	336:360	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	2	2	theme	2.9 Å	584:588	arg1	resolution					590:599	2.9 Å resolution	584:599	2.9 Å resolution	584:599	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	3	theme	endoglucanase	538:550	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	3	theme	endoglucanase	538:550	arg1	CD					509:510	CD	509:510	CD	509:510	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	3	theme	endoglucanase	538:550	arg1	domain					501:506	the glycosylated catalytic domain	474:506	the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	474:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	3	4	with	complex	679:685	arg1	oligosaccharides					705:720	oligosaccharides	705:720	oligosaccharides	705:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	4	with	complex	679:685	arg1	variety					694:700	a variety	692:700	a variety of oligosaccharides	692:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	5	5	theme	decorations	1239:1249	arg1	accommodation					1206:1218	accommodation	1206:1218	accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2	1206:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	3	6	theme	extensive	620:628	arg1	simulations					649:659	our extensive molecular dynamics simulations	616:659	our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides	616:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	5	7	theme	MD	1139:1140	arg1	simulations					1142:1152	MD simulations	1139:1152	MD simulations	1139:1152	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	0	8	from	polysaccharides	159:173	arg1	spectrum					117:124	a wide spectrum	110:124	a wide spectrum of activities on plant cell wall polysaccharides	110:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	4	9	theme	side	948:951	arg1	chain					953:957	a side chain	946:957	a side chain of catalytic Glu196	946:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	5	10	theme	substrates	1258:1267	arg1	accommodation					1206:1218	accommodation	1206:1218	accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2	1206:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	2	11	dep	structure	461:469	arg1	refined					573:579	refined	573:579	refined at 2.9 Å resolution	573:599	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	11	dep	structure	461:469	arg1	solved					562:567	solved	562:567	solved	562:567	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	5	12	theme	binding	1316:1322	arg1	cleft					1324:1328	the binding cleft	1312:1328	the binding cleft close to subsite +2	1312:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	1	13	theme	lignocellulose	255:268	arg1	deconstruction					270:283	plant lignocellulose deconstruction	249:283	plant lignocellulose deconstruction by fungi	249:292	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	3	14	theme	oligosaccharides	705:720	arg1	oligosaccharides					705:720	oligosaccharides	705:720	oligosaccharides	705:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	14	theme	oligosaccharides	705:720	arg1	variety					694:700	a variety	692:700	a variety of oligosaccharides	692:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	15	from	activities	783:792	arg1	polysaccharides					813:827	plant cell wall polysaccharides	797:827	plant cell wall polysaccharides	797:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	5	16	theme	suitable	1193:1200	arg1	pocket					1185:1190	an additional binding pocket	1163:1190	an additional binding pocket	1163:1190	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	3	17	theme	hydrolytic	772:781	arg1	activities					783:792	its promiscuous hydrolytic activities	756:792	its promiscuous hydrolytic activities on plant cell wall polysaccharides	756:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	4	18	from	importance	862:871	arg1	chain					953:957	a side chain	946:957	a side chain of catalytic Glu196	946:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	4	18	from	importance	862:871	arg1	subsite					931:937	subsite -1	931:940	subsite -1	931:940	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	4	18	from	importance	862:871	arg1	Glu196					972:977	catalytic Glu196	962:977	catalytic Glu196	962:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	4	19	theme	hydrogen	880:887	arg1	bond					889:892	the hydrogen bond	876:892	the hydrogen bond between substrate O2 hydroxyl	876:922	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	0	20	theme	wide	112:115	arg1	spectrum					117:124	a wide spectrum	110:124	a wide spectrum of activities on plant cell wall polysaccharides	110:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	5	21	theme	structural	1115:1124	arg1	analyses					1126:1133	detailed structural analyses	1106:1133	detailed structural analyses	1106:1133	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	3	22	from	polysaccharides	813:827	arg1	understanding					739:751	a better understanding	730:751	a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides	730:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	4	23	dep	subsite	931:937	arg1	the					927:929	the	927:929	the	927:929	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	3	24	theme	better	732:737	arg1	understanding					739:751	a better understanding	730:751	a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides	730:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	25	from	understanding	739:751	arg1	polysaccharides					813:827	plant cell wall polysaccharides	797:827	plant cell wall polysaccharides	797:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	2	26	theme	catalytic	491:499	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	26	theme	catalytic	491:499	arg1	CD					509:510	CD	509:510	CD	509:510	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	26	theme	catalytic	491:499	arg1	domain					501:506	the glycosylated catalytic domain	474:506	the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	474:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	27	gly	glycosylated	478:489	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	27	gly	glycosylated	478:489	arg1	CD					509:510	CD	509:510	CD	509:510	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	27	gly	glycosylated	478:489	arg1	domain					501:506	the glycosylated catalytic domain	474:506	the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	474:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	4	28	theme	substrate	902:910	arg1	hydroxyl					915:922	substrate O2 hydroxyl	902:922	substrate O2 hydroxyl	902:922	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	1	29	theme	glycoside	192:200	arg1	family					212:217	glycoside hydrolase family 7	192:219	glycoside hydrolase family 7 (GH7)	192:225	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	1	29	theme	glycoside	192:200	arg1	GH7					222:224	GH7	222:224	GH7	222:224	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	2	30	theme	Trichoderma	516:526	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	5	31	theme	detailed	1106:1113	arg1	analyses					1126:1133	detailed structural analyses	1106:1133	detailed structural analyses	1106:1133	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	0	32	from	spectrum	117:124	arg1	polysaccharides					159:173	plant cell wall polysaccharides	143:173	plant cell wall polysaccharides	143:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	1	33	theme	hydrolase	202:210	arg1	family					212:217	glycoside hydrolase family 7	192:219	glycoside hydrolase family 7 (GH7)	192:225	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	1	33	theme	hydrolase	202:210	arg1	GH7					222:224	GH7	222:224	GH7	222:224	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	5	34	theme	glycoside	1280:1288	arg1	bonds					1290:1294	mixed glycoside bonds	1274:1294	mixed glycoside bonds that abuts onto the binding cleft close to subsite +2	1274:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	0	35	theme	activities	129:138	arg1	spectrum					117:124	a wide spectrum	110:124	a wide spectrum of activities on plant cell wall polysaccharides	110:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	5	36	with	accommodation	1206:1218	arg1	bonds					1290:1294	mixed glycoside bonds	1274:1294	mixed glycoside bonds that abuts onto the binding cleft close to subsite +2	1274:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	2	37	theme	harzianum	528:536	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	3	38	theme	molecular	630:638	arg1	simulations					649:659	our extensive molecular dynamics simulations	616:659	our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides	616:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	4	39	theme	Glu196	972:977	arg1	chain					953:957	a side chain	946:957	a side chain of catalytic Glu196	946:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	4	39	theme	Glu196	972:977	arg1	subsite					931:937	subsite -1	931:940	subsite -1	931:940	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	4	39	theme	Glu196	972:977	arg1	Glu196					972:977	catalytic Glu196	962:977	catalytic Glu196	962:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	5	40	theme	oligosaccharide	1223:1237	arg1	decorations					1239:1249	oligosaccharide decorations	1223:1249	oligosaccharide decorations	1223:1249	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	0	41	theme	molecular	62:70	arg1	adaptations					85:95	molecular architecture adaptations	62:95	molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides	62:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	0	42	theme	plant	143:147	arg1	polysaccharides					159:173	plant cell wall polysaccharides	143:173	plant cell wall polysaccharides	143:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	3	43	theme	dynamics	640:647	arg1	simulations					649:659	our extensive molecular dynamics simulations	616:659	our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides	616:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	2	44	theme	X-ray	455:459	arg1	ThCel7B					553:559	ThCel7B	553:559	ThCel7B	553:559	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	44	theme	X-ray	455:459	arg1	structure					461:469	the X-ray structure	451:469	the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	451:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	1	45	theme	structural	299:308	arg1	information					310:320	structural information	299:320	structural information available for GH7 fungal endoglucanases	299:360	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	1	46	theme	plant	249:253	arg1	deconstruction					270:283	plant lignocellulose deconstruction	249:283	plant lignocellulose deconstruction by fungi	249:292	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	0	47	from	activities	129:138	arg1	polysaccharides					159:173	plant cell wall polysaccharides	143:173	plant cell wall polysaccharides	143:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	1	48	from	number	394:399	arg1	family					427:432	the family	423:432	the family	423:432	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	3	49	theme	activities	783:792	arg1	understanding					739:751	a better understanding	730:751	a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides	730:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	0	50	theme	wall	154:157	arg1	polysaccharides					159:173	plant cell wall polysaccharides	143:173	plant cell wall polysaccharides	143:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	1	51	theme	known	404:408	arg1	sequences					410:418	known sequences	404:418	known sequences in the family	404:432	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	0	52	theme	Cel7B	48:52	arg1	dynamics					14:21	dynamics	14:21	dynamics	14:21	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	0	52	theme	Cel7B	48:52	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	1	53	theme	available	322:330	arg1	information					310:320	structural information	299:320	structural information available for GH7 fungal endoglucanases	299:360	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	0	54	theme	cell	149:152	arg1	polysaccharides					159:173	plant cell wall polysaccharides	143:173	plant cell wall polysaccharides	143:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	1	55	theme	sequences	410:418	arg1	number					394:399	the number	390:399	the number of known sequences in the family	390:432	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	4	56	theme	catalytic	962:970	arg1	Glu196					972:977	catalytic Glu196	962:977	catalytic Glu196	962:977	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	1	57	from	family	212:217	arg1	Cellulases					176:185	Cellulases	176:185	Cellulases from glycoside hydrolase family 7 (GH7)	176:225	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	5	58	theme	binding	1177:1183	arg1	pocket					1185:1190	an additional binding pocket	1163:1190	an additional binding pocket	1163:1190	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	3	59	theme	promiscuous	760:770	arg1	activities					783:792	its promiscuous hydrolytic activities	756:792	its promiscuous hydrolytic activities on plant cell wall polysaccharides	756:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	60	theme	enzyme	669:674	arg1	simulations					649:659	our extensive molecular dynamics simulations	616:659	our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides	616:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	3	61	from	simulations	649:659	arg1	complex					679:685	complex	679:685	complex with a variety of oligosaccharides	679:720	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	2	62	theme	domain	501:506	arg1	ThCel7B					553:559	ThCel7B	553:559	ThCel7B	553:559	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	62	theme	domain	501:506	arg1	structure					461:469	the X-ray structure	451:469	the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	451:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	3	63	theme	cell	803:806	arg1	polysaccharides					813:827	plant cell wall polysaccharides	797:827	plant cell wall polysaccharides	797:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	2	64	theme	glycosylated	478:489	arg1	endoglucanase					538:550	Trichoderma harzianum endoglucanase	516:550	Trichoderma harzianum endoglucanase	516:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	64	theme	glycosylated	478:489	arg1	CD					509:510	CD	509:510	CD	509:510	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	2	64	theme	glycosylated	478:489	arg1	domain					501:506	the glycosylated catalytic domain	474:506	the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase	474:550	Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution.					
30898558	4	65	theme	bond	889:892	arg1	importance					862:871	the importance	858:871	the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides	858:1093	The simulations demonstrate the importance of the hydrogen bond between substrate O2 hydroxyl in the subsite -1 and a side chain of catalytic Glu196 which renders ThCel7B capable to catalytically cleave cello and xylooligosaccharides, but not mannooligosaccharides.					
30898558	3	66	theme	wall	808:811	arg1	polysaccharides					813:827	plant cell wall polysaccharides	797:827	plant cell wall polysaccharides	797:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
30898558	0	67	theme	architecture	72:83	arg1	adaptations					85:95	molecular architecture adaptations	62:95	molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides	62:173	Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.					
30898558	5	68	theme	additional	1166:1175	arg1	pocket					1185:1190	an additional binding pocket	1163:1190	an additional binding pocket	1163:1190	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	5	69	theme	subsite	1339:1345	arg1	+2					1347:1348	subsite +2	1339:1348	subsite +2	1339:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	1	70	theme	crucial	232:238	arg1	roles					240:244	crucial roles	232:244	crucial roles	232:244	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	1	71	from	family	427:432	arg1	number					394:399	the number	390:399	the number of known sequences in the family	390:432	Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family.					
30898558	5	72	theme	mixed	1274:1278	arg1	bonds					1290:1294	mixed glycoside bonds	1274:1294	mixed glycoside bonds that abuts onto the binding cleft close to subsite +2	1274:1348	Moreover, detailed structural analyses and MD simulations revealed an additional binding pocket, suitable for accommodation of oligosaccharide decorations and/or substrates with mixed glycoside bonds that abuts onto the binding cleft close to subsite +2.					
30898558	3	73	theme	plant	797:801	arg1	polysaccharides					813:827	plant cell wall polysaccharides	797:827	plant cell wall polysaccharides	797:827	Additionally, our extensive molecular dynamics simulations of this enzyme in complex with a variety of oligosaccharides provide a better understanding of its promiscuous hydrolytic activities on plant cell wall polysaccharides.					
31400421	4	0	theme	DPPH	966:969	arg1	radicals					971:978	DPPH radicals	966:978	DPPH radicals	966:978	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	1	1	theme	respective	306:315	arg1	parameters					322:331	best parameters	317:331	their respective best parameters	300:331	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	8	2	theme	ginger	1492:1497	arg1	source					1535:1540	a potential source	1523:1540	a potential source for natural antioxidant	1523:1564	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	8	2	theme	ginger	1492:1497	arg1	pomace					1499:1504	the ginger pomace	1488:1504	the ginger pomace	1488:1504	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	3	3	contain	possessed	629:637	arg1	Meanwhile					600:608	Meanwhile	600:608	Meanwhile	600:608	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	3	contain	possessed	629:637	arg1	kinds					615:619	two kinds	611:619	two kinds of GPPs	611:627	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	3	contain	possessed	629:637	arg2	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	3	contain	possessed	629:637	arg2	characteristics					676:690	the different preliminary structural characteristics	639:690	the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content,	639:786	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	1	4	dep	polysaccharide	400:413	arg1	HW-GPP1					416:422	HW-GPP1	416:422	HW-GPP1	416:422	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	7	5	theme	potential	1444:1452	arg1	antioxidant					1462:1472	a potential natural antioxidant	1442:1472	a potential natural antioxidant	1442:1472	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	7	5	theme	potential	1444:1452	arg1	UA-GPPs					1417:1423	UA-GPPs	1417:1423	UA-GPPs	1417:1423	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	1	6	theme	best	317:320	arg1	parameters					322:331	best parameters	317:331	their respective best parameters	300:331	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	2	7	theme	polysaccharide	542:555	arg1	higher					576:581	higher	576:581	higher	576:581	The data implied that the yield of the polysaccharide obtained by UA was higher than that of HW.					
31400421	2	7	theme	polysaccharide	542:555	arg1	yield					529:533	the yield	525:533	the yield of the polysaccharide obtained by UA	525:570	The data implied that the yield of the polysaccharide obtained by UA was higher than that of HW.					
31400421	6	8	theme	polysaccharides	1320:1334	arg1	yields					1306:1311	the extraction yields	1291:1311	the extraction yields of the polysaccharides	1291:1334	These results showed that UA was more beneficial to enhance the extraction yields of the polysaccharides, and also resulted in GPPs with higher bioactivity.					
31400421	4	9	from	abilities	1030:1038	arg1	radicals					1067:1074	hydroxyl and superoxide radicals	1043:1074	radicals	1067:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	8	10	used	used	1515:1518	arg2	pomace					1499:1504	the ginger pomace	1488:1504	the ginger pomace	1488:1504	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	8	10	used	used	1515:1518	arg2	source					1535:1540	a potential source	1523:1540	a potential source for natural antioxidant	1523:1564	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	0	11	theme	extraction	109:118	arg1	processes					120:128	two different extraction processes	95:128	two different extraction processes	95:128	Characterization and antioxidant activity of polysaccharides obtained from ginger pomace using two different extraction processes.					
31400421	3	12	theme	sulfuric	834:841	arg1	contents					851:858	sulfuric radical contents	834:858	sulfuric radical contents	834:858	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	1	13	theme	polysaccharide	239:252	arg1	extraction					225:234	the extraction	221:234	the extraction of polysaccharide from ginger pomace (GPPs)	221:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	0	14	theme	different	99:107	arg1	processes					120:128	two different extraction processes	95:128	two different extraction processes	95:128	Characterization and antioxidant activity of polysaccharides obtained from ginger pomace using two different extraction processes.					
31400421	3	15	theme	preliminary	653:663	arg1	characteristics					676:690	the different preliminary structural characteristics	639:690	the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content,	639:786	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	15	theme	preliminary	653:663	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	16	theme	acid	774:777	arg1	content					779:785	uronic acid content	767:785	uronic acid content	767:785	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	16	theme	acid	774:777	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	4	17	theme	strongest	932:940	arg1	abilities					953:961	the strongest scavenging abilities	928:961	the strongest scavenging abilities on DPPH radicals	928:978	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	3	18	theme	structural	665:674	arg1	characteristics					676:690	the different preliminary structural characteristics	639:690	the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content,	639:786	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	18	theme	structural	665:674	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	1	19	theme	hot	173:175	arg1	HW					184:185	HW	184:185	HW	184:185	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	19	theme	hot	173:175	arg1	water					177:181	hot water	173:181	hot water (HW)	173:186	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	5	20	theme	antioxidant	1091:1101	arg1	better					1158:1163	better	1158:1163	better	1158:1163	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	5	20	theme	antioxidant	1091:1101	arg1	activity					1103:1110	the antioxidant activity	1087:1110	the antioxidant activity of each fractions of GPPs extracted by UA	1087:1152	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	7	21	used	used	1434:1437	arg2	antioxidant					1462:1472	a potential natural antioxidant	1442:1472	a potential natural antioxidant	1442:1472	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	7	21	used	used	1434:1437	arg2	UA-GPPs					1417:1423	UA-GPPs	1417:1423	UA-GPPs	1417:1423	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	7	22	theme	natural	1454:1460	arg1	antioxidant					1462:1472	a potential natural antioxidant	1442:1472	a potential natural antioxidant	1442:1472	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	7	22	theme	natural	1454:1460	arg1	UA-GPPs					1417:1423	UA-GPPs	1417:1423	UA-GPPs	1417:1423	Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant.					
31400421	1	23	theme	water	177:181	arg1	processes					160:168	two different processes	146:168	two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs)	146:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	3	24	theme	different	643:651	arg1	characteristics					676:690	the different preliminary structural characteristics	639:690	the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content,	639:786	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	24	theme	different	643:651	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	25	theme	molecular	702:710	arg1	content					758:764	total sugar and protein content	734:764	content	758:764	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	25	theme	molecular	702:710	arg1	content					779:785	uronic acid content	767:785	uronic acid content	767:785	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	25	theme	molecular	702:710	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	25	theme	molecular	702:710	arg1	sugar					740:744	total sugar and protein content	734:764	sugar	740:744	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	5	26	theme	fractions	1120:1128	arg1	better					1158:1163	better	1158:1163	better	1158:1163	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	5	26	theme	fractions	1120:1128	arg1	activity					1103:1110	the antioxidant activity	1087:1110	the antioxidant activity of each fractions of GPPs extracted by UA	1087:1152	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	1	27	theme	ginger	259:264	arg1	GPPs					274:277	GPPs	274:277	GPPs	274:277	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	27	theme	ginger	259:264	arg1	pomace					266:271	ginger pomace	259:271	ginger pomace (GPPs)	259:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	28	from	pomace	266:271	arg1	extraction					225:234	the extraction	221:234	the extraction of polysaccharide from ginger pomace (GPPs)	221:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	4	29	contain	possessed	995:1003	arg2	abilities					1030:1038	the strongest scavenging abilities	1005:1038	the strongest scavenging abilities on hydroxyl and superoxide radicals	1005:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	4	29	contain	possessed	995:1003	arg1	UA-GPP2					987:993	UA-GPP2	987:993	UA-GPP2	987:993	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	4	30	theme	scavenging	1019:1028	arg1	abilities					1030:1038	the strongest scavenging abilities	1005:1038	the strongest scavenging abilities on hydroxyl and superoxide radicals	1005:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	3	31	theme	weight	712:717	arg1	content					758:764	total sugar and protein content	734:764	content	758:764	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	31	theme	weight	712:717	arg1	content					779:785	uronic acid content	767:785	uronic acid content	767:785	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	31	theme	weight	712:717	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	31	theme	weight	712:717	arg1	sugar					740:744	total sugar and protein content	734:764	sugar	740:744	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	0	32	theme	antioxidant	21:31	arg1	activity					33:40	antioxidant activity	21:40	antioxidant activity	21:40	Characterization and antioxidant activity of polysaccharides obtained from ginger pomace using two different extraction processes.					
31400421	4	33	theme	activity	882:889	arg1	assays					891:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	4	34	dep	In	861:862	arg1	vitro					864:868	vitro	864:868	vitro	864:868	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	0	35	theme	polysaccharides	45:59	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antioxidant activity of polysaccharides obtained from ginger pomace using two different extraction processes.					
31400421	0	35	theme	polysaccharides	45:59	arg1	activity					33:40	antioxidant activity	21:40	antioxidant activity	21:40	Characterization and antioxidant activity of polysaccharides obtained from ginger pomace using two different extraction processes.					
31400421	6	36	theme	extraction	1295:1304	arg1	yields					1306:1311	the extraction yields	1291:1311	the extraction yields of the polysaccharides	1291:1334	These results showed that UA was more beneficial to enhance the extraction yields of the polysaccharides, and also resulted in GPPs with higher bioactivity.					
31400421	1	37	theme	antioxidant	363:373	arg1	activity					375:382	antioxidant activity	363:382	antioxidant activity	363:382	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	38	theme	ultrasonic-assisted	192:210	arg1	processes					160:168	two different processes	146:168	two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs)	146:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	3	39	theme	total	734:738	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	39	theme	total	734:738	arg1	sugar					740:744	total sugar and protein content	734:764	sugar	740:744	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	6	40	theme	higher	1368:1373	arg1	bioactivity					1375:1385	higher bioactivity	1368:1385	higher bioactivity	1368:1385	These results showed that UA was more beneficial to enhance the extraction yields of the polysaccharides, and also resulted in GPPs with higher bioactivity.					
31400421	5	41	theme	GPPs	1133:1136	arg1	fractions					1120:1128	each fractions	1115:1128	each fractions of GPPs extracted by UA	1115:1152	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	4	42	theme	In	861:862	arg1	assays					891:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	4	43	theme	scavenging	942:951	arg1	abilities					953:961	the strongest scavenging abilities	928:961	the strongest scavenging abilities on DPPH radicals	928:978	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	8	44	theme	natural	1546:1552	arg1	antioxidant					1554:1564	natural antioxidant	1546:1564	natural antioxidant	1546:1564	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	8	45	theme	potential	1525:1533	arg1	source					1535:1540	a potential source	1523:1540	a potential source for natural antioxidant	1523:1564	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	8	45	theme	potential	1525:1533	arg1	pomace					1499:1504	the ginger pomace	1488:1504	the ginger pomace	1488:1504	Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.					
31400421	6	46	with	GPPs	1358:1361	arg1	bioactivity					1375:1385	higher bioactivity	1368:1385	higher bioactivity	1368:1385	These results showed that UA was more beneficial to enhance the extraction yields of the polysaccharides, and also resulted in GPPs with higher bioactivity.					
31400421	3	47	theme	monosaccharide	802:815	arg1	compositions					817:828	similar monosaccharide compositions	794:828	similar monosaccharide compositions	794:828	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	4	48	theme	antioxidant	870:880	arg1	assays					891:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays	861:896	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	1	49	theme	purified	391:398	arg1	polysaccharide					400:413	the purified polysaccharide	387:413	the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively)	387:486	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	5	50	theme	corresponding	1182:1194	arg1	fractions					1196:1204	the corresponding fractions	1178:1204	the corresponding fractions of GPPs extracted by HW	1178:1228	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	4	51	theme	superoxide	1056:1065	arg1	radicals					1067:1074	hydroxyl and superoxide radicals	1043:1074	radicals	1067:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	1	52	theme	polysaccharide	400:413	arg1	activity					375:382	antioxidant activity	363:382	antioxidant activity	363:382	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	52	theme	polysaccharide	400:413	arg1	characterization					342:357	the characterization	338:357	the characterization	338:357	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	53	dep	HW-GPP1	416:422	arg1	HW-GPP2					425:431	HW-GPP2	425:431	HW-GPP2	425:431	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	53	dep	HW-GPP1	416:422	arg1	UA-GPP3					465:471	UA-GPP3	465:471	UA-GPP3	465:471	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	53	dep	HW-GPP1	416:422	arg1	UA-GPP2					456:462	UA-GPP2	456:462	UA-GPP2	456:462	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	53	dep	HW-GPP1	416:422	arg1	HW-GPP3					434:440	HW-GPP3	434:440	HW-GPP3	434:440	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	1	53	dep	HW-GPP1	416:422	arg1	UA-GPP1					447:453	UA-GPP1	447:453	UA-GPP1	447:453	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	5	54	theme	GPPs	1209:1212	arg1	fractions					1196:1204	the corresponding fractions	1178:1204	the corresponding fractions of GPPs extracted by HW	1178:1228	Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW.					
31400421	4	55	theme	hydroxyl	1043:1050	arg1	radicals					1067:1074	hydroxyl and superoxide radicals	1043:1074	radicals	1067:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	3	56	theme	similar	794:800	arg1	compositions					817:828	similar monosaccharide compositions	794:828	similar monosaccharide compositions	794:828	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	4	57	from	abilities	953:961	arg1	radicals					971:978	DPPH radicals	966:978	DPPH radicals	966:978	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	3	58	theme	uronic	767:772	arg1	content					779:785	uronic acid content	767:785	uronic acid content	767:785	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	58	theme	uronic	767:772	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	59	theme	GPPs	624:627	arg1	Meanwhile					600:608	Meanwhile	600:608	Meanwhile	600:608	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	59	theme	GPPs	624:627	arg1	kinds					615:619	two kinds	611:619	two kinds of GPPs	611:627	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	4	60	theme	strongest	1009:1017	arg1	abilities					1030:1038	the strongest scavenging abilities	1005:1038	the strongest scavenging abilities on hydroxyl and superoxide radicals	1005:1074	In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals.					
31400421	1	61	theme	different	150:158	arg1	processes					160:168	two different processes	146:168	two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs)	146:278	In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed.					
31400421	3	62	theme	radical	843:849	arg1	contents					851:858	sulfuric radical contents	834:858	sulfuric radical contents	834:858	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	63	theme	protein	750:756	arg1	content					758:764	total sugar and protein content	734:764	content	758:764	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
31400421	3	63	theme	protein	750:756	arg1	distributions					719:731	molecular weight distributions	702:731	molecular weight distributions	702:731	Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents.					
30716975	19	0	theme	polymer	2996:3002	arg1	possibility					3043:3053	a technological possibility to produce sensorily appealing high-quality non-alcoholic beers	3027:3117	a technological possibility to produce sensorily appealing high-quality non-alcoholic beers	3027:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	19	0	theme	polymer	2996:3002	arg1	profiles					3004:3011	polymer profiles	2996:3011	polymer profiles	2996:3011	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	19	1	theme	profiles	3004:3011	arg1	composition					2981:2991	the composition	2977:2991	the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers	2977:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	11	2	theme	Limited	1663:1669	arg1	beers					1684:1688	Limited fermentation beers	1663:1688	Limited fermentation beers	1663:1688	Limited fermentation beers tended to be composed of more low-molar-mass polymers.					
30716975	3	3	theme	non-alcoholic	526:538	arg1	beers					540:544	non-alcoholic beers	526:544	non-alcoholic beers	526:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	12	4	from	differences	1833:1843	arg1	profile					1863:1869	macromolecular profile	1848:1869	macromolecular profile	1848:1869	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	12	4	from	differences	1833:1843	arg1	impressions					1892:1902	specific sensory impressions	1875:1902	specific sensory impressions like sweetness (basic taste), palate fullness and harmony	1875:1960	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	7	5	from	variations	1142:1151	arg1	profile					1164:1170	polymer profile	1156:1170	polymer profile	1156:1170	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	7	5	from	variations	1142:1151	arg1	method					1187:1192	processing method	1176:1192	processing method	1176:1192	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	19	6	theme	technological	3029:3041	arg1	possibility					3043:3053	a technological possibility to produce sensorily appealing high-quality non-alcoholic beers	3027:3117	a technological possibility to produce sensorily appealing high-quality non-alcoholic beers	3027:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	19	6	theme	technological	3029:3041	arg1	profiles					3004:3011	polymer profiles	2996:3011	polymer profiles	2996:3011	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	18	7	theme	sensory	2910:2916	arg1	impressions					2918:2928	sensory impressions	2910:2928	sensory impressions	2910:2928	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	8	8	theme	light-scattering	1270:1285	arg1	measurements					1316:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	12	9	theme	palate	1934:1939	arg1	fullness					1941:1948	palate fullness	1934:1948	palate fullness	1934:1948	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	4	10	theme	significant	682:692	arg1	factor					694:699	a significant factor	680:699	a significant factor	680:699	In addition to technological aspects, the matrix components represent a significant factor.					
30716975	16	11	with	spiking	2588:2594	arg1	β-glucan					2601:2608	β-glucan	2601:2608	β-glucan	2601:2608	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	7	12	theme	processing	1176:1185	arg1	method					1187:1192	processing method	1176:1192	processing method	1176:1192	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	10	13	theme	distribution	1527:1538	arg1	shift					1503:1507	a shift	1501:1507	a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column	1501:1660	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	8	14	used	used	1334:1337	arg2	measurements					1316:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	1	15	theme	fastest-growing	172:186	arg1	market					188:193	the fastest-growing market	168:193	the fastest-growing market within the beverage industry	168:222	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	1	15	theme	fastest-growing	172:186	arg1	beer					160:163	non-alcoholic beer	146:163	non-alcoholic beer	146:163	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	16	16	theme	perceived	2644:2652	arg1	mouthfeel					2654:2662	a more viscous, thick perceived mouthfeel	2622:2662	a more viscous, thick perceived mouthfeel	2622:2662	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	14	17	theme	cereal-based	2339:2350	arg1	substances					2352:2361	cereal-based substances	2339:2361	cereal-based substances	2339:2361	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	10	18	theme	molar	1516:1520	arg1	distribution					1527:1538	the molar mass distribution	1512:1538	the molar mass distribution	1512:1538	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	2	19	theme	matrix	330:335	arg1	compositions					337:348	completely different matrix compositions	309:348	completely different matrix compositions	309:348	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	9	20	from	differences	1412:1422	arg1	ratio					1431:1435	the ratio	1427:1435	the ratio of low- to high-molar-mass polymers	1427:1471	We observed significant differences in the ratio of low- to high-molar-mass polymers.					
30716975	12	21	theme	basic	1920:1924	arg1	sweetness					1909:1917	sweetness	1909:1917	sweetness (basic taste)	1909:1931	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	12	21	theme	basic	1920:1924	arg1	taste					1926:1930	basic taste	1920:1930	basic taste	1920:1930	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	7	22	from	differences	1075:1085	arg1	mouthfeel					1110:1118	mouthfeel	1110:1118	mouthfeel	1110:1118	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	7	22	from	differences	1075:1085	arg1	fullness					1097:1104	palate fullness	1090:1104	palate fullness	1090:1104	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	1	23	theme	beverage	206:213	arg1	industry					215:222	the beverage industry	202:222	the beverage industry	202:222	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	8	24	theme	refractive	1299:1308	arg1	index					1310:1314	refractive index	1299:1314	refractive index	1299:1314	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	5	25	theme	various	754:760	arg1	polymers					762:769	various polymers	754:769	various polymers	754:769	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	7	26	theme	palate	1090:1095	arg1	fullness					1097:1104	palate fullness	1090:1104	palate fullness	1090:1104	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	19	27	theme	high-quality	3086:3097	arg1	beers					3113:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	2	28	theme	Different	225:233	arg1	technologies					246:257	Different processing technologies	225:257	Different processing technologies for limiting the alcohol content of beer	225:298	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	1	29	theme	changing	120:127	arg1	habits					138:143	changing consumer habits	120:143	changing consumer habits	120:143	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	12	30	theme	sensory	1884:1890	arg1	impressions					1892:1902	specific sensory impressions	1875:1902	specific sensory impressions like sweetness (basic taste), palate fullness and harmony	1875:1960	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	11	31	theme	fermentation	1671:1682	arg1	beers					1684:1688	Limited fermentation beers	1663:1688	Limited fermentation beers	1663:1688	Limited fermentation beers tended to be composed of more low-molar-mass polymers.					
30716975	2	32	theme	alcohol	276:282	arg1	content					284:290	the alcohol content	272:290	the alcohol content of beer	272:298	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	13	33	theme	palate	2091:2096	arg1	fullness					2098:2105	palate fullness	2091:2105	palate fullness	2091:2105	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	0	34	theme	non-alcoholic	62:74	arg1	beers					76:80	non-alcoholic beers	62:80	non-alcoholic beers	62:80	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	13	35	theme	production	2216:2225	arg1	process					2227:2233	the production process	2212:2233	the production process	2212:2233	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	7	36	theme	beers	1050:1054	arg1	profile					1025:1031	the macromolecular profile	1006:1031	the macromolecular profile of non-alcoholic beers	1006:1054	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	1	37	theme	non-alcoholic	146:158	arg1	market					188:193	the fastest-growing market	168:193	the fastest-growing market within the beverage industry	168:222	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	1	37	theme	non-alcoholic	146:158	arg1	beer					160:163	non-alcoholic beer	146:163	non-alcoholic beer	146:163	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	12	38	theme	macromolecular	1848:1861	arg1	profile					1863:1869	macromolecular profile	1848:1869	macromolecular profile	1848:1869	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	18	39	attach	linked	2900:2905	arg1	impressions					2918:2928	sensory impressions	2910:2928	sensory impressions	2910:2928	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	18	39	attach	linked	2900:2905	arg2	fractions					2860:2868	the macromolecular fractions	2841:2868	the macromolecular fractions in cereal-based beverages	2841:2894	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	10	40	from	dealcoholization	1619:1634	arg1	column					1655:1660	a rectification column	1639:1660	a rectification column	1639:1660	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	3	41	theme	specific	387:394	arg1	attributes					404:413	Especially the specific sensory attributes	372:413	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	372:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	10	42	theme	higher	1548:1553	arg1	masses					1561:1566	higher molar masses	1548:1566	higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column	1548:1660	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	18	43	theme	cereal-based	2873:2884	arg1	beverages					2886:2894	cereal-based beverages	2873:2894	cereal-based beverages	2873:2894	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	0	44	theme	various	96:102	arg1	methods					104:110	various methods	96:110	various methods	96:110	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	16	45	dep	viscous	2629:2635	arg1	thick					2638:2642	thick	2638:2642	thick	2638:2642	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	7	46	theme	macromolecular	1010:1023	arg1	profile					1025:1031	the macromolecular profile	1006:1031	the macromolecular profile of non-alcoholic beers	1006:1054	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	18	47	theme	suitable	2794:2801	arg1	tool					2814:2817	a suitable analytical tool	2792:2817	a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions	2792:2928	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	18	47	theme	suitable	2794:2801	arg1	AF4-MALS					2780:2787	AF4-MALS	2780:2787	AF4-MALS	2780:2787	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	18	48	from	fractions	2860:2868	arg1	beverages					2886:2894	cereal-based beverages	2873:2894	cereal-based beverages	2873:2894	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	3	49	theme	palate	418:423	arg1	fullness					425:432	palate fullness	418:432	palate fullness	418:432	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	17	50	theme	basic	2729:2733	arg1	taste					2722:2726	a sweet taste	2714:2726	a sweet taste (basic taste)	2714:2740	However, spiking with isomaltulose led solely to a sweet taste (basic taste).					
30716975	17	50	theme	basic	2729:2733	arg1	taste					2735:2739	basic taste	2729:2739	basic taste	2729:2739	However, spiking with isomaltulose led solely to a sweet taste (basic taste).					
30716975	7	51	theme	analytical	975:984	arg1	characterization					986:1001	the analytical characterization	971:1001	the analytical characterization of the macromolecular profile of non-alcoholic beers	971:1054	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	12	52	theme	assessment	1777:1786	arg1	schemes					1788:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	13	53	theme	present	1993:1999	arg1	study					2001:2005	the present study	1989:2005	the present study	1989:2005	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	7	54	theme	sensory	1067:1073	arg1	differences					1075:1085	sensory differences	1067:1085	sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method	1067:1192	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	10	55	theme	thermal	1598:1604	arg1	loads					1606:1610	higher thermal loads	1591:1610	higher thermal loads	1591:1610	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	6	56	theme	mouthfeel	899:907	arg1	terms					890:894	terms	890:894	terms of mouthfeel	890:907	These polymers affect the sensory perception of beverages in terms of mouthfeel depending on their substance properties.					
30716975	5	57	contain	contain	725:731	arg2	mixture					743:749	a complex mixture	733:749	a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides	733:826	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	5	57	contain	contain	725:731	arg1	beverages					715:723	Cereal-based beverages	702:723	Cereal-based beverages	702:723	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	10	58	theme	rectification	1641:1653	arg1	column					1655:1660	a rectification column	1639:1660	a rectification column	1639:1660	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	11	59	theme	low-molar-mass	1720:1733	arg1	polymers					1735:1742	more low-molar-mass polymers	1715:1742	more low-molar-mass polymers	1715:1742	Limited fermentation beers tended to be composed of more low-molar-mass polymers.					
30716975	0	60	theme	profile	51:57	arg1	Characterization					0:15	Characterization	0:15	Characterization of the macromolecular and sensory profile of non-alcoholic beers	0:80	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	3	61	dep	harmony	468:474	arg1	harmony					483:489	ratio harmony	477:489	ratio harmony between sweetness and sourness	477:520	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	16	62	theme	mouthfeel	2577:2585	arg1	perception					2563:2572	a lasting, smooth, and pleasant perception	2531:2572	a lasting, smooth, and pleasant perception of mouthfeel	2531:2585	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	2	63	theme	sensory	354:360	arg1	profiles					362:369	sensory profiles	354:369	sensory profiles	354:369	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	6	64	theme	beverages	877:885	arg1	perception					863:872	the sensory perception	851:872	the sensory perception of beverages in terms of mouthfeel	851:907	These polymers affect the sensory perception of beverages in terms of mouthfeel depending on their substance properties.					
30716975	13	65	dep	such	2108:2111	arg1	controlled					2194:2203	controlled	2194:2203	may be controlled during the production process	2187:2233	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	3	66	theme	beers	540:544	arg1	harmony					468:474	harmony	468:474	harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	468:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	8	67	theme	asymmetric	1206:1215	arg1	FFF/AF4					1248:1254	FFF/AF4	1248:1254	FFF/AF4	1248:1254	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	8	67	theme	asymmetric	1206:1215	arg1	flow-field-flow-fractionation					1217:1245	asymmetric flow-field-flow-fractionation	1206:1245	asymmetric flow-field-flow-fractionation (FFF/AF4)	1206:1255	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	19	68	theme	assessment	2944:2953	arg1	type					2936:2939	This type	2931:2939	This type of assessment	2931:2953	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	14	69	theme	molar	2392:2396	arg1	mass					2398:2401	their molar mass	2386:2401	their molar mass	2386:2401	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	13	70	theme	other	2158:2162	arg1	sugars					2179:2184	other low-molar-mass sugars	2158:2184	other low-molar-mass sugars	2158:2184	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	15	71	theme	palate	2481:2486	arg1	fullness					2488:2495	palate fullness	2481:2495	palate fullness	2481:2495	Maltodextrins and β-glucan are shown to enhance palate fullness.					
30716975	16	72	theme	viscous	2629:2635	arg1	mouthfeel					2654:2662	a more viscous, thick perceived mouthfeel	2622:2662	a more viscous, thick perceived mouthfeel	2622:2662	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	16	73	theme	smooth	2542:2547	arg1	perception					2563:2572	a lasting, smooth, and pleasant perception	2531:2572	a lasting, smooth, and pleasant perception of mouthfeel	2531:2585	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	6	74	theme	sensory	855:861	arg1	perception					863:872	the sensory perception	851:872	the sensory perception of beverages in terms of mouthfeel	851:907	These polymers affect the sensory perception of beverages in terms of mouthfeel depending on their substance properties.					
30716975	17	75	with	spiking	2674:2680	arg1	isomaltulose					2687:2698	isomaltulose	2687:2698	isomaltulose	2687:2698	However, spiking with isomaltulose led solely to a sweet taste (basic taste).					
30716975	16	76	theme	lasting	2533:2539	arg1	perception					2563:2572	a lasting, smooth, and pleasant perception	2531:2572	a lasting, smooth, and pleasant perception of mouthfeel	2531:2585	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	2	77	theme	beer	295:298	arg1	content					284:290	the alcohol content	272:290	the alcohol content of beer	272:298	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	8	78	theme	multi-angle	1258:1268	arg1	MALS					1288:1291	MALS	1288:1291	MALS	1288:1291	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	8	78	theme	multi-angle	1258:1268	arg1	light-scattering					1270:1285	multi-angle light-scattering	1258:1285	multi-angle light-scattering (MALS)	1258:1292	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	6	79	theme	substance	928:936	arg1	properties					938:947	their substance properties	922:947	their substance properties	922:947	These polymers affect the sensory perception of beverages in terms of mouthfeel depending on their substance properties.					
30716975	14	80	theme	substances	2352:2361	arg1	classes					2328:2334	different classes	2318:2334	different classes of cereal-based substances	2318:2361	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	7	81	theme	polymer	1156:1162	arg1	profile					1164:1170	polymer profile	1156:1170	polymer profile	1156:1170	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	10	82	theme	mass	1522:1525	arg1	distribution					1527:1538	the molar mass distribution	1512:1538	the molar mass distribution	1512:1538	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	16	83	theme	pleasant	2554:2561	arg1	perception					2563:2572	a lasting, smooth, and pleasant perception	2531:2572	a lasting, smooth, and pleasant perception of mouthfeel	2531:2585	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	5	84	theme	complex	735:741	arg1	mixture					743:749	a complex mixture	733:749	a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides	733:826	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	2	85	theme	different	320:328	arg1	compositions					337:348	completely different matrix compositions	309:348	completely different matrix compositions	309:348	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	12	86	theme	discriminating	1752:1765	arg1	schemes					1788:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	14	87	theme	different	2318:2326	arg1	classes					2328:2334	different classes	2318:2334	different classes of cereal-based substances	2318:2361	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	8	88	theme	non-alcoholic	1367:1379	arg1	beers					1381:1385	non-alcoholic beers	1367:1385	non-alcoholic beers	1367:1385	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	19	89	theme	appealing	3076:3084	arg1	beers					3113:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	5	90	theme	Cereal-based	702:713	arg1	beverages					715:723	Cereal-based beverages	702:723	Cereal-based beverages	702:723	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	2	91	theme	processing	235:244	arg1	technologies					246:257	Different processing technologies	225:257	Different processing technologies for limiting the alcohol content of beer	225:298	Different processing technologies for limiting the alcohol content of beer yield in completely different matrix compositions and sensory profiles.					
30716975	8	92	theme	index	1310:1314	arg1	measurements					1316:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
30716975	14	93	theme	sensory	2278:2284	arg1	attributes					2286:2295	the particular sensory attributes	2263:2295	the particular sensory attributes	2263:2295	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	4	94	dep	aspects	639:645	arg1	addition					613:620	addition	613:620	addition	613:620	In addition to technological aspects, the matrix components represent a significant factor.					
30716975	1	95	theme	consumer	129:136	arg1	habits					138:143	changing consumer habits	120:143	changing consumer habits	120:143	Due to changing consumer habits, non-alcoholic beer is the fastest-growing market within the beverage industry.					
30716975	12	96	theme	specific	1875:1882	arg1	impressions					1892:1902	specific sensory impressions	1875:1902	specific sensory impressions like sweetness (basic taste), palate fullness and harmony	1875:1960	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	18	97	theme	macromolecular	2845:2858	arg1	fractions					2860:2868	the macromolecular fractions	2841:2868	the macromolecular fractions in cereal-based beverages	2841:2894	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	0	98	theme	beers	76:80	arg1	profile					51:57	the macromolecular and sensory profile	20:57	the macromolecular and sensory profile of non-alcoholic beers	20:80	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	4	99	theme	matrix	652:657	arg1	components					659:668	the matrix components	648:668	the matrix components	648:668	In addition to technological aspects, the matrix components represent a significant factor.					
30716975	10	100	theme	molar	1555:1559	arg1	masses					1561:1566	higher molar masses	1548:1566	higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column	1548:1660	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	5	101	theme	polymers	762:769	arg1	mixture					743:749	a complex mixture	733:749	a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides	733:826	Cereal-based beverages contain a complex mixture of various polymers that includes proteins, polyphenols, and polysaccharides.					
30716975	9	102	theme	significant	1400:1410	arg1	differences					1412:1422	significant differences	1400:1422	significant differences in the ratio of low- to high-molar-mass polymers	1400:1471	We observed significant differences in the ratio of low- to high-molar-mass polymers.					
30716975	7	103	theme	non-alcoholic	1036:1048	arg1	beers					1050:1054	non-alcoholic beers	1036:1054	non-alcoholic beers	1036:1054	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	3	104	theme	sensory	396:402	arg1	attributes					404:413	Especially the specific sensory attributes	372:413	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	372:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	14	105	theme	particular	2267:2276	arg1	attributes					2286:2295	the particular sensory attributes	2263:2295	the particular sensory attributes	2263:2295	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	17	106	theme	sweet	2716:2720	arg1	taste					2722:2726	a sweet taste	2714:2726	a sweet taste (basic taste)	2714:2740	However, spiking with isomaltulose led solely to a sweet taste (basic taste).					
30716975	17	106	theme	sweet	2716:2720	arg1	taste					2735:2739	basic taste	2729:2739	basic taste	2729:2739	However, spiking with isomaltulose led solely to a sweet taste (basic taste).					
30716975	13	107	theme	spiking	2012:2018	arg1	experiments					2020:2030	spiking experiments	2012:2030	spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process	2012:2233	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	18	108	theme	analytical	2803:2812	arg1	tool					2814:2817	a suitable analytical tool	2792:2817	a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions	2792:2928	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	18	108	theme	analytical	2803:2812	arg1	AF4-MALS					2780:2787	AF4-MALS	2780:2787	AF4-MALS	2780:2787	These tests show more generally that AF4-MALS is a suitable analytical tool for understanding how the macromolecular fractions in cereal-based beverages are linked to sensory impressions.					
30716975	7	109	theme	profile	1025:1031	arg1	characterization					986:1001	the analytical characterization	971:1001	the analytical characterization of the macromolecular profile of non-alcoholic beers	971:1054	This article reports the analytical characterization of the macromolecular profile of non-alcoholic beers to predict sensory differences in palate fullness and mouthfeel that arise because of variations in polymer profile and processing method.					
30716975	14	110	dep	mass	2398:2401	arg1	fractions					2403:2411	fractions	2403:2411	fractions	2403:2411	These results confirm that the particular sensory attributes can be influenced by different classes of cereal-based substances, especially by varying their molar mass fractions and concentrations.					
30716975	19	111	theme	non-alcoholic	3099:3111	arg1	beers					3113:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	sensorily appealing high-quality non-alcoholic beers	3066:3117	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	3	112	theme	harmony	468:474	arg1	mouthfeel					435:443	mouthfeel	435:443	mouthfeel	435:443	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	3	112	theme	harmony	468:474	arg1	fullness					425:432	palate fullness	418:432	palate fullness	418:432	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	3	112	theme	harmony	468:474	arg1	perception					454:463	the perception	450:463	the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	450:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	6	113	from	perception	863:872	arg1	terms					890:894	terms	890:894	terms of mouthfeel	890:907	These polymers affect the sensory perception of beverages in terms of mouthfeel depending on their substance properties.					
30716975	3	114	theme	fullness	425:432	arg1	attributes					404:413	Especially the specific sensory attributes	372:413	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	372:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	3	115	theme	perception	454:463	arg1	attributes					404:413	Especially the specific sensory attributes	372:413	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	372:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	0	116	theme	macromolecular	24:37	arg1	profile					51:57	the macromolecular and sensory profile	20:57	the macromolecular and sensory profile of non-alcoholic beers	20:80	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	9	117	theme	low-	1440:1443	arg1	ratio					1431:1435	the ratio	1427:1435	the ratio of low- to high-molar-mass polymers	1427:1471	We observed significant differences in the ratio of low- to high-molar-mass polymers.					
30716975	19	118	used	used	2962:2965	arg2	type					2936:2939	This type	2931:2939	This type of assessment	2931:2953	This type of assessment can be used to target the composition of polymer profiles, which can be a technological possibility to produce sensorily appealing high-quality non-alcoholic beers.					
30716975	12	119	theme	sensorial	1767:1775	arg1	schemes					1788:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes	1745:1794	Highly discriminating sensorial assessment schemes demonstrate the correlations between differences in macromolecular profile and specific sensory impressions like sweetness (basic taste), palate fullness and harmony.					
30716975	0	120	theme	sensory	43:49	arg1	profile					51:57	the macromolecular and sensory profile	20:57	the macromolecular and sensory profile of non-alcoholic beers	20:80	Characterization of the macromolecular and sensory profile of non-alcoholic beers produced with various methods.					
30716975	9	121	theme	high-molar-mass	1448:1462	arg1	polymers					1464:1471	high-molar-mass polymers	1448:1471	high-molar-mass polymers	1448:1471	We observed significant differences in the ratio of low- to high-molar-mass polymers.					
30716975	10	122	theme	higher	1591:1596	arg1	loads					1606:1610	higher thermal loads	1591:1610	higher thermal loads	1591:1610	These differences included a shift of the molar mass distribution towards higher molar masses for beers subjected to higher thermal loads during dealcoholization in a rectification column.					
30716975	13	123	theme	low-molar-mass	2164:2177	arg1	sugars					2179:2184	other low-molar-mass sugars	2158:2184	other low-molar-mass sugars	2158:2184	This trend is verified in the present study with spiking experiments using substances that are known to influence mouthfeel and palate fullness, such as beta-glucans, dextrins, isomaltulose, and other low-molar-mass sugars, may be controlled during the production process.					
30716975	3	124	theme	ratio	477:481	arg1	harmony					483:489	ratio harmony	477:489	ratio harmony between sweetness and sourness	477:520	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	3	125	theme	mouthfeel	435:443	arg1	attributes					404:413	Especially the specific sensory attributes	372:413	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers	372:544	Especially the specific sensory attributes of palate fullness, mouthfeel, and the perception of harmony (ratio harmony between sweetness and sourness) of non-alcoholic beers are often described as atypical and unbalanced by the consumer.					
30716975	16	126	with	Spiking	2498:2504	arg1	maltodextrin					2511:2522	maltodextrin	2511:2522	maltodextrin	2511:2522	Spiking with maltodextrin caused a lasting, smooth, and pleasant perception of mouthfeel; spiking with β-glucan resulted in a more viscous, thick perceived mouthfeel.					
30716975	4	127	theme	technological	625:637	arg1	aspects					639:645	technological aspects	625:645	technological aspects	625:645	In addition to technological aspects, the matrix components represent a significant factor.					
30716975	8	128	theme	flow-field-flow-fractionation	1217:1245	arg1	measurements					1316:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements	1206:1327	Therefore, asymmetric flow-field-flow-fractionation (FFF/AF4), multi-angle light-scattering (MALS), and refractive index measurements were used to characterize polymers in non-alcoholic beers.					
31002897	4	0	theme	FS	841:842	arg1	films					844:848	FS films	841:848	FS films	841:848	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	8	1	theme	regeneration	1396:1407	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	1	theme	regeneration	1396:1407	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	2	theme	such	1282:1285	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	2	theme	such	1282:1285	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	3	theme	periodontal	1472:1482	arg1	tissues					1484:1490	periodontal tissues	1472:1490	periodontal tissues	1472:1490	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	4	theme	bone	1450:1453	arg1	defects					1455:1461	small bone defects	1444:1461	small bone defects	1444:1461	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	6	5	from	immersion	1031:1039	arg1	fluid					1059:1063	simulated body fluid	1044:1063	simulated body fluid	1044:1063	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	3	6	theme	antibacterial	416:428	arg1	FS					445:446	FS	445:446	FS	445:446	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	6	theme	antibacterial	416:428	arg1	free-standing					430:442	novel antibacterial free-standing	410:442	novel antibacterial free-standing (FS) films	410:453	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	5	7	theme	weight	905:910	arg1	morphology					865:874	surface morphology	857:874	surface morphology	857:874	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	7	theme	weight	905:910	arg1	loss					912:915	weight loss	905:915	weight loss	905:915	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	7	8	theme	direct	1143:1148	arg1	contact					1150:1156	direct contact	1143:1156	direct contact with Staphylococcus aureus and Escherichia coli cultures	1143:1213	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	8	9	theme	temporary	1374:1382	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	9	theme	temporary	1374:1382	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	3	10	theme	hyaluronic	519:528	arg1	polymers					483:490	natural polymers	475:490	natural polymers	475:490	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	10	theme	hyaluronic	519:528	arg1	HA					536:537	HA	536:537	HA	536:537	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	10	theme	hyaluronic	519:528	arg1	acid					530:533	hyaluronic acid	519:533	hyaluronic acid (HA)	519:538	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	8	11	theme	bone	1391:1394	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	11	theme	bone	1391:1394	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	4	12	theme	bioglass	698:705	arg1	nanoparticles					707:719	silver doped bioglass nanoparticles	685:719	silver doped bioglass nanoparticles (Ag-BGs)	685:728	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	4	12	theme	bioglass	698:705	arg1	Ag-BGs					722:727	Ag-BGs	722:727	Ag-BGs	722:727	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	4	13	theme	films	844:848	arg1	formulations					825:836	four distinct formulations	811:836	four distinct formulations of FS films	811:848	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	8	14	theme	bioactive	1287:1295	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	14	theme	bioactive	1287:1295	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	7	15	theme	FS	1222:1223	arg1	films					1225:1229	these FS films	1216:1229	these FS films	1216:1229	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	8	16	used	used	1366:1369	arg2	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	16	used	used	1366:1369	arg2	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	1	17	theme	different	183:191	arg1	substrates					204:213	different underwater substrates	183:213	different underwater substrates	183:213	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	6	18	theme	simulated	1044:1052	arg1	fluid					1059:1063	simulated body fluid	1044:1063	simulated body fluid	1044:1063	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	8	19	theme	small	1444:1448	arg1	defects					1455:1461	small bone defects	1444:1461	small bone defects	1444:1461	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	1	20	theme	adhesive	84:91	arg1	well-known					136:145	well-known	136:145	well-known	136:145	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	1	20	theme	adhesive	84:91	arg1	capabilities					93:104	The adhesive capabilities	80:104	The adhesive capabilities of marine mussel proteins	80:130	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	6	21	theme	body	1054:1057	arg1	fluid					1059:1063	simulated body fluid	1044:1063	simulated body fluid	1044:1063	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	2	22	theme	catechol	386:393	arg1	group					395:399	the reactive catechol group	373:399	the reactive catechol group	373:399	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	0	23	theme	free-standing	14:26	arg1	films					53:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films inspired by the sea.					
31002897	6	24	theme	layer	1113:1117	arg1	formation					1080:1088	the formation	1076:1088	the formation of a bone-like apatite layer	1076:1117	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	2	25	theme	acid	314:317	arg1	levels					298:303	the high levels	289:303	the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group	289:399	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	2	26	theme	reactive	377:384	arg1	group					395:399	the reactive catechol group	373:399	the reactive catechol group	373:399	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	0	27	theme	Antibacterial	0:12	arg1	films					53:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films inspired by the sea.					
31002897	4	28	theme	distinct	816:823	arg1	formulations					825:836	four distinct formulations	811:836	four distinct formulations of FS films	811:848	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	2	29	theme	amino	308:312	arg1	3,4-dihydroxyphenyl-l-alanine					320:348	3,4-dihydroxyphenyl-l-alanine	320:348	3,4-dihydroxyphenyl-l-alanine (DOPA)	320:355	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	2	29	theme	amino	308:312	arg1	acid					314:317	amino acid	308:317	amino acid	308:317	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	3	30	theme	free-standing	430:442	arg1	films					449:453	novel antibacterial free-standing (FS) films	410:453	novel antibacterial free-standing (FS) films	410:453	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	0	31	theme	composite	43:51	arg1	films					53:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films inspired by the sea.					
31002897	4	32	theme	doped	692:696	arg1	nanoparticles					707:719	silver doped bioglass nanoparticles	685:719	silver doped bioglass nanoparticles (Ag-BGs)	685:728	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	4	32	theme	doped	692:696	arg1	Ag-BGs					722:727	Ag-BGs	722:727	Ag-BGs	722:727	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	1	33	theme	marine	109:114	arg1	proteins					123:130	marine mussel proteins	109:130	marine mussel proteins	109:130	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	7	34	theme	antibacterial	1249:1261	arg1	effect					1263:1268	a clear antibacterial effect	1241:1268	a clear antibacterial effect	1241:1268	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	0	35	theme	polysaccharide	28:41	arg1	films					53:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films	0:57	Antibacterial free-standing polysaccharide composite films inspired by the sea.					
31002897	7	36	from	16 h	1135:1138	arg1	contact					1150:1156	direct contact	1143:1156	direct contact with Staphylococcus aureus and Escherichia coli cultures	1143:1213	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	1	37	theme	mussel	116:121	arg1	proteins					123:130	marine mussel proteins	109:130	marine mussel proteins	109:130	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	8	38	theme	free-standing	1325:1337	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	38	theme	free-standing	1325:1337	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	6	39	theme	apatite	1105:1111	arg1	layer					1113:1117	a bone-like apatite layer	1093:1117	a bone-like apatite layer	1093:1117	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	7	40	theme	clear	1243:1247	arg1	effect					1263:1268	a clear antibacterial effect	1241:1268	a clear antibacterial effect	1241:1268	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	7	41	dep	aureus	1178:1183	arg1	cultures					1206:1213	cultures	1206:1213	cultures	1206:1213	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	3	42	theme	catechol-functionalized	551:573	arg1	HA-DN					592:596	HA-DN	592:596	HA-DN	592:596	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	42	theme	catechol-functionalized	551:573	arg1	acid					586:589	the catechol-functionalized hyaluronic acid	547:589	the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties	547:646	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	1	43	theme	underwater	193:202	arg1	substrates					204:213	different underwater substrates	183:213	different underwater substrates	183:213	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	3	44	theme	novel	410:414	arg1	FS					445:446	FS	445:446	FS	445:446	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	44	theme	novel	410:414	arg1	free-standing					430:442	novel antibacterial free-standing	410:442	novel antibacterial free-standing (FS) films	410:453	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	45	theme	hyaluronic	575:584	arg1	HA-DN					592:596	HA-DN	592:596	HA-DN	592:596	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	45	theme	hyaluronic	575:584	arg1	acid					586:589	the catechol-functionalized hyaluronic acid	547:589	the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties	547:646	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	1	46	theme	inorganic	223:231	arg1	substrates					204:213	different underwater substrates	183:213	different underwater substrates	183:213	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	6	47	theme	bioactivity	994:1004	arg1	tests					1006:1010	bioactivity tests	994:1010	bioactivity tests	994:1010	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	8	48	theme	adhesive	1316:1323	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	48	theme	adhesive	1316:1323	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	1	49	theme	proteins	123:130	arg1	well-known					136:145	well-known	136:145	well-known	136:145	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	1	49	theme	proteins	123:130	arg1	capabilities					93:104	The adhesive capabilities	80:104	The adhesive capabilities of marine mussel proteins	80:130	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	7	50	with	contact	1150:1156	arg1	aureus					1178:1183	Staphylococcus aureus	1163:1183	Staphylococcus aureus	1163:1183	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	7	50	with	contact	1150:1156	arg1	coli					1201:1204	Escherichia coli	1189:1204	Escherichia coli	1189:1204	Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect.					
31002897	4	51	theme	composite	668:676	arg1	films					678:682	composite films	668:682	composite films	668:682	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	4	52	theme	bactericidal	759:770	arg1	properties					786:795	bactericidal and bioactive properties	759:795	bactericidal and bioactive properties	759:795	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	8	53	theme	guided	1384:1389	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	53	theme	guided	1384:1389	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	4	54	theme	bioactive	776:784	arg1	properties					786:795	bactericidal and bioactive properties	759:795	bactericidal and bioactive properties	759:795	In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films.					
31002897	6	55	theme	bone-like	1095:1103	arg1	layer					1113:1117	a bone-like apatite layer	1093:1117	a bone-like apatite layer	1093:1117	In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer.					
31002897	8	56	theme	antibacterial	1298:1310	arg1	films					1409:1413	temporary guided bone regeneration films	1374:1413	temporary guided bone regeneration films	1374:1413	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	8	56	theme	antibacterial	1298:1310	arg1	films					1339:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	such bioactive, antibacterial and adhesive free-standing films	1282:1343	Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.					
31002897	3	57	theme	wet	624:626	arg1	properties					637:646	wet adhesive properties	624:646	wet adhesive properties	624:646	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	2	58	theme	high	293:296	arg1	levels					298:303	the high levels	289:303	the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group	289:399	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	3	59	theme	natural	475:481	arg1	polymers					483:490	natural polymers	475:490	natural polymers	475:490	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	59	theme	natural	475:481	arg1	chitosan					500:507	chitosan	500:507	chitosan (CHT)	500:513	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	3	59	theme	natural	475:481	arg1	acid					530:533	hyaluronic acid	519:533	hyaluronic acid (HA)	519:538	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	1	60	theme	organic	236:242	arg1	substrates					204:213	different underwater substrates	183:213	different underwater substrates	183:213	The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic.					
31002897	3	61	theme	adhesive	628:635	arg1	properties					637:646	wet adhesive properties	624:646	wet adhesive properties	624:646	Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties.					
31002897	2	62	theme	adhesive	258:265	arg1	properties					267:276	These unique adhesive properties	245:276	These unique adhesive properties	245:276	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	2	63	theme	unique	251:256	arg1	properties					267:276	These unique adhesive properties	245:276	These unique adhesive properties	245:276	These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group.					
31002897	5	64	theme	surface	857:863	arg1	wettability					892:902	wettability	892:902	wettability	892:902	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	mechanical					928:937	mechanical	928:937	mechanical	928:937	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	adhesion					940:947	adhesion	940:947	adhesion	940:947	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	morphology					865:874	surface morphology	857:874	surface morphology	857:874	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	loss					912:915	weight loss	905:915	weight loss	905:915	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	swelling					918:925	swelling	918:925	swelling	918:925	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31002897	5	64	theme	surface	857:863	arg1	bioactivity					953:963	bioactivity	953:963	bioactivity	953:963	Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed.					
31892825	4	0	with	miraculin	750:758	arg1	activity					807:814	the same taste-modifying activity	782:814	the same taste-modifying activity	782:814	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	5	1	theme	a	1467:1467	arg1	epitope					1469:1475	a Lewis a epitope	1459:1475	a Lewis a epitope	1459:1475	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	2	theme	miraculins	369:378	arg1	activities					343:352	the activities	339:352	the activities of recombinant miraculins	339:378	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	2	2	theme	miraculins	369:378	arg1	lower					390:394	lower	390:394	lower	390:394	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	2	3	from	difference	448:457	arg1	modification					481:492	post-translational modification	462:492	post-translational modification	462:492	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	4	4	theme	N-glycan	885:892	arg1	structures					894:903	the N-glycan structures	881:903	the N-glycan structures	881:903	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	6	5	theme	taste-modifying	1750:1764	arg1	activity					1766:1773	the full taste-modifying activity	1741:1773	the full taste-modifying activity of miraculin	1741:1786	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	2	6	theme	recombinant	357:367	arg1	miraculins					369:378	recombinant miraculins	357:378	recombinant miraculins	357:378	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	5	7	theme	chromatography	1107:1120	arg1	spectrometry					1134:1145	a liquid chromatography tandem-mass spectrometry	1098:1145	a liquid chromatography tandem-mass spectrometry analysis	1098:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	0	8	from	Comparison	0:9	arg1	miraculin					49:57	recombinant miraculin	37:57	recombinant miraculin	37:57	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	0	9	with	plants	79:84	arg1	miraculin					98:106	native miraculin	91:106	native miraculin	91:106	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	4	10	theme	taste-modifying	791:805	arg1	activity					807:814	the same taste-modifying activity	782:814	the same taste-modifying activity	782:814	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	3	11	theme	recombinant	588:598	arg1	miraculin					600:608	recombinant miraculin	588:608	recombinant miraculin	588:608	For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized.					
31892825	0	12	theme	native	91:96	arg1	miraculin					98:106	native miraculin	91:106	native miraculin	91:106	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	4	13	theme	same	786:789	arg1	activity					807:814	the same taste-modifying activity	782:814	the same taste-modifying activity	782:814	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	3	14	theme	practical	527:535	arg1	use					537:539	practical use	527:539	practical use therefore	527:549	For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized.					
31892825	5	15	theme	PA	1040:1041	arg1	-labeling					1043:1051	2-pyridylamine (PA)-labeling	1024:1051	2-pyridylamine (PA)-labeling	1024:1051	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	16	theme	full	1745:1748	arg1	activity					1766:1773	the full taste-modifying activity	1741:1773	the full taste-modifying activity of miraculin	1741:1786	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	5	17	theme	β1,2-xylosylated	1420:1435	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	4	18	theme	miracle	850:856	arg1	fruit					858:862	miracle fruit	850:862	miracle fruit	850:862	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	5	19	gly	α1,3-fucosylated	1396:1411	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	1	20	with	protein	134:140	arg1	properties					163:172	taste-modifying properties	147:172	taste-modifying properties	147:172	Miraculin is a promising protein with taste-modifying properties.					
31892825	6	21	with	identical	1625:1633	arg1	ratios					1662:1667	similar ratios	1654:1667	similar ratios	1654:1667	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	5	22	theme	2-pyridylamine	1024:1037	arg1	-labeling					1043:1051	2-pyridylamine (PA)-labeling	1024:1051	2-pyridylamine (PA)-labeling	1024:1051	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	23	theme	N-glycans	1437:1445	arg1	amount					1371:1376	a smaller amount	1361:1376	a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1361:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	23	theme	N-glycans	1437:1445	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	24	theme	high-performance	1054:1069	arg1	chromatography					1078:1091	high-performance liquid chromatography	1054:1091	high-performance liquid chromatography	1054:1091	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	25	dep	difference	448:457	arg1	N-glycosylation					506:520	N-glycosylation	506:520	N-glycosylation	506:520	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	3	26	theme	miraculin	600:608	arg1	N-glycan					576:583	N-glycan	576:583	N-glycan of recombinant miraculin compared to that of native miraculin	576:645	For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized.					
31892825	5	27	theme	recombinant	1190:1200	arg1	miraculins					1202:1211	both the native and recombinant miraculins	1170:1211	miraculins	1202:1211	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	28	theme	liquid	1100:1105	arg1	spectrometry					1134:1145	a liquid chromatography tandem-mass spectrometry	1098:1145	a liquid chromatography tandem-mass spectrometry analysis	1098:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	29	theme	systems	326:332	arg1	use					295:297	the use	291:297	the use of heterologous expression systems	291:332	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	0	30	theme	N-glycosylation	18:32	arg1	Comparison					0:9	Comparison	0:9	Comparison of the N-glycosylation on recombinant miraculin	0:57	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	5	31	dep	miraculins	1202:1211	arg1	both					1170:1173	both	1170:1173	both	1170:1173	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	32	from	N-glycoform	1510:1520	arg1	fruit					1555:1559	miracle fruit	1547:1559	miracle fruit	1547:1559	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	32	from	N-glycoform	1510:1520	arg1	miraculin					1577:1585	recombinant miraculin	1565:1585	recombinant miraculin	1565:1585	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	2	33	theme	expression	315:324	arg1	systems					326:332	heterologous expression systems	302:332	heterologous expression systems	302:332	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	6	34	theme	miraculin	1532:1540	arg1	identical					1625:1633	identical	1625:1633	identical	1625:1633	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	34	theme	miraculin	1532:1540	arg1	N-glycoform					1510:1520	the N-glycoform	1506:1520	the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1506:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	4	35	theme	tomato	763:768	arg1	plants					770:775	tomato plants	763:775	tomato plants	763:775	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	5	36	theme	predominant	1242:1252	arg1	structure					1227:1235	an M3 structure	1221:1235	an M3 structure	1221:1235	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	36	theme	predominant	1242:1252	arg1	structure					1254:1262	a predominant structure	1240:1262	a predominant structure	1240:1262	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	37	theme	unique	191:196	arg1	function					198:205	unique function	191:205	unique function	191:205	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	2	38	theme	heterologous	302:313	arg1	systems					326:332	heterologous expression systems	302:332	heterologous expression systems	302:332	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	5	39	theme	plant-specific	1381:1394	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	40	gly	N-glycoform	1510:1520	arg1	miraculin					1532:1540	native miraculin	1525:1540	native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1525:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	0	41	theme	recombinant	37:47	arg1	miraculin					49:57	recombinant miraculin	37:57	recombinant miraculin	37:57	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	6	42	from	miraculin	1577:1585	arg1	identical					1625:1633	identical	1625:1633	identical	1625:1633	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	42	from	miraculin	1577:1585	arg1	N-glycoform					1510:1520	the N-glycoform	1506:1520	the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1506:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	42	from	miraculin	1577:1585	arg1	miraculin					1532:1540	native miraculin	1525:1540	native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1525:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	43	theme	miracle	1547:1553	arg1	fruit					1555:1559	miracle fruit	1547:1559	miracle fruit	1547:1559	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	5	44	theme	tandem-mass	1122:1132	arg1	spectrometry					1134:1145	a liquid chromatography tandem-mass spectrometry	1098:1145	a liquid chromatography tandem-mass spectrometry analysis	1098:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	45	theme	miraculin	247:255	arg1	production					257:266	recombinant miraculin production	235:266	recombinant miraculin production	235:266	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	5	46	theme	α1,3-fucosylated	1396:1411	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	47	theme	spectrometry	1134:1145	arg1	analysis					1147:1154	a liquid chromatography tandem-mass spectrometry analysis	1098:1154	a liquid chromatography tandem-mass spectrometry analysis	1098:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	2	48	theme	recombinant	235:245	arg1	production					257:266	recombinant miraculin production	235:266	recombinant miraculin production	235:266	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	6	49	theme	plant-specific	1690:1703	arg1	N-glycans					1705:1713	plant-specific N-glycans	1690:1713	plant-specific N-glycans	1690:1713	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	2	50	theme	native	410:415	arg1	miraculin					417:425	native miraculin	410:425	native miraculin	410:425	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	2	51	theme	post-translational	462:479	arg1	modification					481:492	post-translational modification	462:492	post-translational modification	462:492	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	5	52	from	structures	1294:1303	arg1	miraculins					1312:1321	the miraculins	1308:1321	the miraculins	1308:1321	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	53	theme	liquid	1071:1076	arg1	chromatography					1078:1091	high-performance liquid chromatography	1054:1091	high-performance liquid chromatography	1054:1091	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	54	with	structures	1345:1354	arg1	amount					1371:1376	a smaller amount	1361:1376	a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1361:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	54	with	structures	1345:1354	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	55	theme	N-glycan	941:948	arg1	analysis					961:968	Our N-glycan structural analysis	937:968	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis	937:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	56	theme	M3	1224:1225	arg1	structure					1227:1235	an M3 structure	1221:1235	an M3 structure	1221:1235	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	56	theme	M3	1224:1225	arg1	structure					1254:1262	a predominant structure	1240:1262	a predominant structure	1240:1262	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	4	57	theme	native	919:924	arg1	miraculin					926:934	native miraculin	919:934	native miraculin	919:934	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	2	58	theme	miraculin	224:232	arg1	potential					211:219	potential	211:219	potential	211:219	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	2	58	theme	miraculin	224:232	arg1	function					198:205	unique function	191:205	unique function	191:205	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	5	59	theme	structural	950:959	arg1	analysis					961:968	Our N-glycan structural analysis	937:968	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis	937:1154	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	60	theme	tomato	1600:1605	arg1	plants					1607:1612	tomato plants	1600:1612	tomato plants	1600:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	5	61	theme	N-glycan	1285:1292	arg1	structures					1294:1303	the N-glycan structures	1281:1303	the N-glycan structures on the miraculins	1281:1321	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	62	theme	purified	976:983	arg1	miraculin					985:993	purified miraculin	976:993	purified miraculin	976:993	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	63	theme	miraculin	1778:1786	arg1	activity					1766:1773	the full taste-modifying activity	1741:1773	the full taste-modifying activity of miraculin	1741:1786	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	64	from	fruit	1555:1559	arg1	identical					1625:1633	identical	1625:1633	identical	1625:1633	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	64	from	fruit	1555:1559	arg1	N-glycoform					1510:1520	the N-glycoform	1506:1520	the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1506:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	6	64	from	fruit	1555:1559	arg1	miraculin					1532:1540	native miraculin	1525:1540	native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1525:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	3	65	theme	native	630:635	arg1	miraculin					637:645	native miraculin	630:645	native miraculin	630:645	For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized.					
31892825	1	66	theme	promising	124:132	arg1	protein					134:140	a promising protein	122:140	a promising protein with taste-modifying properties	122:172	Miraculin is a promising protein with taste-modifying properties.					
31892825	1	66	theme	promising	124:132	arg1	Miraculin					109:117	Miraculin	109:117	Miraculin	109:117	Miraculin is a promising protein with taste-modifying properties.					
31892825	4	67	from	miraculin	750:758	arg1	plants					770:775	tomato plants	763:775	tomato plants	763:775	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	6	68	theme	native	1525:1530	arg1	miraculin					1532:1540	native miraculin	1525:1540	native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants	1525:1612	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	5	69	contain	carried	1213:1219	arg2	structure					1254:1262	a predominant structure	1240:1262	a predominant structure	1240:1262	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	69	contain	carried	1213:1219	arg2	structure					1227:1235	an M3 structure	1221:1235	an M3 structure	1221:1235	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	69	contain	carried	1213:1219	arg1	miraculins					1202:1211	both the native and recombinant miraculins	1170:1211	miraculins	1202:1211	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	6	70	theme	similar	1654:1660	arg1	ratios					1662:1667	similar ratios	1654:1667	similar ratios	1654:1667	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
31892825	4	71	theme	native	819:824	arg1	miraculin					750:758	miraculin	750:758	miraculin in tomato plants with the same taste-modifying activity	750:814	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	4	71	theme	native	819:824	arg1	miraculin					826:834	native miraculin	819:834	native miraculin purified from miracle fruit	819:862	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	4	72	theme	functional	704:713	arg1	production					725:734	functional miraculin production	704:734	functional miraculin production	704:734	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	2	73	dep	function	198:205	arg1	the					187:189	the	187:189	the	187:189	Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation.					
31892825	0	74	theme	tomato	72:77	arg1	plants					79:84	tomato plants	72:84	tomato plants with native miraculin	72:106	Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin.					
31892825	5	75	theme	pauci-mannosidic	1328:1343	arg1	structures					1345:1354	pauci-mannosidic structures	1328:1354	pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope	1328:1475	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	4	76	theme	miraculin	715:723	arg1	production					725:734	functional miraculin production	704:734	functional miraculin production	704:734	Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin.					
31892825	5	77	theme	smaller	1363:1369	arg1	amount					1371:1376	a smaller amount	1361:1376	a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1361:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	77	theme	smaller	1363:1369	arg1	N-glycans					1437:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans	1381:1445	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	78	theme	native	1179:1184	arg1	miraculins					1202:1211	both the native and recombinant miraculins	1170:1211	miraculins	1202:1211	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	5	79	theme	Lewis	1461:1465	arg1	epitope					1469:1475	a Lewis a epitope	1459:1475	a Lewis a epitope	1459:1475	Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope.					
31892825	1	80	theme	taste-modifying	147:161	arg1	properties					163:172	taste-modifying properties	147:172	taste-modifying properties	147:172	Miraculin is a promising protein with taste-modifying properties.					
31892825	6	81	theme	recombinant	1565:1575	arg1	miraculin					1577:1585	recombinant miraculin	1565:1585	recombinant miraculin	1565:1585	These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.					
30965075	6	0	theme	Preliminary	870:880	arg1	study					892:896	Preliminary mechanism study	870:896	Preliminary mechanism study	870:896	Preliminary mechanism study indicated that it interacted with C1q, C2, C3, C4 and C5.					
30965075	0	1	theme	3-O-methyl	95:104	arg1	galactose					106:114	3-O-methyl galactose	95:114	3-O-methyl galactose from Juniperus tibetica	95:138	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	7	2	theme	scientific	1050:1059	arg1	bases					1061:1065	scientific bases	1050:1065	scientific bases for YB-PS4	1050:1076	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	7	2	theme	scientific	1050:1059	arg1	inhibitor					1094:1102	a complement inhibitor	1081:1102	a complement inhibitor	1081:1102	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	3	3	theme	-α-Galp-	495:502	arg1	-α-GalpA-					515:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	4	dep	1→	525:526	arg1	1→					600:601	1→	600:601	1→	600:601	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	4	dep	1→	525:526	arg1	→2					581:582	→2	581:582	→2	581:582	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	4	dep	1→	525:526	arg1	1 →					573:575	1 →	573:575	1 →	573:575	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	7	5	theme	tibetica	1028:1035	arg1	constituents					1009:1020	the active constituents	998:1020	the active constituents of J. tibetica	998:1035	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	3	6	dep	had	428:430	arg1	1→					525:526	1→	525:526	1→	525:526	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	4	7	theme	repetitive	617:626	arg1	units					628:632	The possible repetitive units	604:632	The possible repetitive units	604:632	The possible repetitive units were speculated and further analyzed by oligosaccharide analysis.					
30965075	4	8	theme	possible	608:615	arg1	units					628:632	The possible repetitive units	604:632	The possible repetitive units	604:632	The possible repetitive units were speculated and further analyzed by oligosaccharide analysis.					
30965075	5	9	theme	inhibitory	714:723	arg1	effects					725:731	inhibitory effects	714:731	inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL)	714:867	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	5	10	from	effects	725:731	arg1	activation					747:756	complement activation	736:756	complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL)	736:867	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	7	11	theme	J.	1025:1026	arg1	tibetica					1028:1035	J. tibetica	1025:1035	J. tibetica	1025:1035	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	4	12	theme	oligosaccharide	674:688	arg1	analysis					690:697	oligosaccharide analysis	674:697	oligosaccharide analysis	674:697	The possible repetitive units were speculated and further analyzed by oligosaccharide analysis.					
30965075	1	13	contain	containing	179:188	arg2	galactose					201:209	3-O-methyl galactose	190:209	3-O-methyl galactose	190:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	1	13	contain	containing	179:188	arg1	polysaccharide					164:177	A water-soluble acidic polysaccharide	141:177	A water-soluble acidic polysaccharide containing 3-O-methyl galactose	141:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	7	14	theme	active	1002:1007	arg1	constituents					1009:1020	the active constituents	998:1020	the active constituents of J. tibetica	998:1035	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	0	15	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	3	16	contain	had	428:430	arg1	YB-PS4					421:426	YB-PS4	421:426	YB-PS4	421:426	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	16	contain	had	428:430	arg2	-α-GalpA-					515:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	17	dep	-α-Galp-	495:502	arg1	→2,4					490:493	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	→2,4	490:493	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	17	dep	-α-Galp-	495:502	arg1	→4					512:513	→4	512:513	→4	512:513	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	17	dep	-α-Galp-	495:502	arg1	1 →					504:506	1 →	504:506	1 →	504:506	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	18	theme	-α-Rhap-	459:466	arg1	-α-Galp-					495:502	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	-α-Galp-	495:502	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	0	19	theme	anticomplement	32:45	arg1	activity					47:54	anticomplement activity	32:54	anticomplement activity	32:54	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	2	20	theme	composition	342:352	arg1	analysis					354:361	monosaccharide composition analysis	327:361	monosaccharide composition analysis	327:361	Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy.					
30965075	7	21	theme	complement	1083:1092	arg1	bases					1061:1065	scientific bases	1050:1065	scientific bases for YB-PS4	1050:1076	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	7	21	theme	complement	1083:1092	arg1	inhibitor					1094:1102	a complement inhibitor	1081:1102	a complement inhibitor	1081:1102	These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.					
30965075	3	22	theme	-α-Araf-	564:571	arg1	branches					534:541	branches	534:541	branches of →2)-α-Rhap-(1→,→3)-α-Araf-	534:571	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	2	23	theme	monosaccharide	327:340	arg1	analysis					354:361	monosaccharide composition analysis	327:361	monosaccharide composition analysis	327:361	Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy.					
30965075	3	24	theme	-α-Araf-	477:484	arg1	-α-Galp-					495:502	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	-α-Galp-	495:502	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	25	theme	composed	443:450	arg1	-α-Galp-					495:502	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	-α-Galp-	495:502	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	5	26	theme	classical	770:778	arg1	CH50 = 94.23 ± 8.90 μg/mL					789:813	CH50 = 94.23 ± 8.90 μg/mL	789:813	CH50 = 94.23 ± 8.90 μg/mL	789:813	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	5	26	theme	classical	770:778	arg1	pathway					780:786	the classical pathway	766:786	the classical pathway (CH50 = 94.23 ± 8.90 μg/mL)	766:814	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	3	27	theme	of→2,4	452:457	arg1	-α-Galp-					495:502	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	-α-Galp-	495:502	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	5	28	theme	complement	736:745	arg1	activation					747:756	complement activation	736:756	complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL)	736:867	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	0	29	theme	acidic	62:67	arg1	polysaccharide					69:82	an acidic polysaccharide	59:82	an acidic polysaccharide	59:82	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	2	30	theme	NMR	381:383	arg1	spectroscopy					385:396	NMR spectroscopy	381:396	NMR spectroscopy	381:396	Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy.					
30965075	3	31	theme	backbone	434:441	arg1	-α-GalpA-					515:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-	432:523	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	1	32	attach	isolated	230:237	arg2	polysaccharide					164:177	A water-soluble acidic polysaccharide	141:177	A water-soluble acidic polysaccharide containing 3-O-methyl galactose	141:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	1	32	attach	isolated	230:237	arg1	twigs					248:252	the twigs	244:252	the twigs	244:252	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	1	33	theme	water-soluble	143:155	arg1	polysaccharide					164:177	A water-soluble acidic polysaccharide	141:177	A water-soluble acidic polysaccharide containing 3-O-methyl galactose	141:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	0	34	from	tibetica	131:138	arg1	galactose					106:114	3-O-methyl galactose	95:114	3-O-methyl galactose from Juniperus tibetica	95:138	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	3	35	theme	1→	558:559	arg1	-α-Araf-					564:571	→2)-α-Rhap-(1→,→3)-α-Araf-	546:571	-α-Araf-	564:571	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	1	36	theme	acidic	157:162	arg1	polysaccharide					164:177	A water-soluble acidic polysaccharide	141:177	A water-soluble acidic polysaccharide containing 3-O-methyl galactose	141:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
30965075	3	37	theme	1→	486:487	arg1	-α-Galp-					495:502	composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-	443:502	-α-Galp-	495:502	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	3	38	theme	-α-Rhap-	549:556	arg1	-α-Araf-					564:571	→2)-α-Rhap-(1→,→3)-α-Araf-	546:571	-α-Araf-	564:571	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	5	39	theme	alternative	820:830	arg1	AP50 = 194.76 ± 9.20 μg/mL					841:866	AP50 = 194.76 ± 9.20 μg/mL	841:866	AP50 = 194.76 ± 9.20 μg/mL	841:866	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	5	39	theme	alternative	820:830	arg1	pathway					832:838	alternative pathway	820:838	alternative pathway (AP50 = 194.76 ± 9.20 μg/mL)	820:867	YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL).					
30965075	3	40	dep	-α-Araf-	564:571	arg1	→3					561:562	→2)-α-Rhap-(1→,→3)-α-Araf-	546:571	→3	561:562	It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→.					
30965075	0	41	theme	polysaccharide	69:82	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	0	41	theme	polysaccharide	69:82	arg1	activity					47:54	anticomplement activity	32:54	anticomplement activity	32:54	Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica.					
30965075	6	42	theme	mechanism	882:890	arg1	study					892:896	Preliminary mechanism study	870:896	Preliminary mechanism study	870:896	Preliminary mechanism study indicated that it interacted with C1q, C2, C3, C4 and C5.					
30965075	1	43	theme	3-O-methyl	190:199	arg1	galactose					201:209	3-O-methyl galactose	190:209	3-O-methyl galactose	190:209	A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom.					
31377617	0	0	theme	Craterellus	91:101	arg1	tubaeformis					103:113	wild Finnish mushroom Craterellus tubaeformis	69:113	wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)	69:134	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	0	0	theme	Craterellus	91:101	arg1	Chanterelle					123:133	Funnel Chanterelle	116:133	Funnel Chanterelle	116:133	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	8	1	theme	little	1121:1126	arg1	impact					1128:1133	little impact	1121:1133	little impact on the thermal stability	1121:1158	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	8	2	theme	%	999:999	arg1	use					987:989	The use	983:989	The use of KOH 2%	983:999	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	7	3	theme	polysaccharide	957:970	arg1	fractions					972:980	the polysaccharide fractions	953:980	the polysaccharide fractions	953:980	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	6	4	dep	consisted	751:759	arg1	1 → chains					822:831	1 → chains	822:831	1 → chains	822:831	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	6	4	dep	consisted	751:759	arg1	residues					849:856	single β-Glc residues	836:856	single β-Glc residues	836:856	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	0	5	theme	mushroom	82:89	arg1	tubaeformis					103:113	wild Finnish mushroom Craterellus tubaeformis	69:113	wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)	69:134	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	0	5	theme	mushroom	82:89	arg1	Chanterelle					123:133	Funnel Chanterelle	116:133	Funnel Chanterelle	116:133	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	5	6	theme	water	599:603	arg1	extract					605:611	The hot water extract	591:611	The hot water extract	591:611	The hot water extract consisted mainly of high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains, covalently bound to proteins.					
31377617	8	7	theme	bimodal	1076:1082	arg1	distributions					1101:1113	bimodal molecular weight distributions	1076:1113	bimodal molecular weight distributions	1076:1113	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	2	8	theme	fruiting	304:311	arg1	bodies					313:318	the fruiting bodies	300:318	the fruiting bodies of C. tubaeformis	300:336	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	8	9	theme	thermal	1142:1148	arg1	stability					1150:1158	the thermal stability	1138:1158	the thermal stability	1138:1158	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	7	10	theme	thermal	932:938	arg1	linkages					903:910	the glycosidic linkages	888:910	the glycosidic linkages	888:910	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	10	theme	thermal	932:938	arg1	stability					940:948	thermal stability	932:948	thermal stability	932:948	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	3	11	theme	spectroscopic	518:530	arg1	methods					532:538	chromatographic and spectroscopic methods	498:538	chromatographic and spectroscopic methods	498:538	The monomer composition, molecular weight, and chemical structure were determined using chromatographic and spectroscopic methods.					
31377617	6	12	dep	-β-Glc-	775:781	arg1	1→					783:784	1→	783:784	1→	783:784	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	3	13	theme	monomer	414:420	arg1	composition					422:432	The monomer composition	410:432	The monomer composition	410:432	The monomer composition, molecular weight, and chemical structure were determined using chromatographic and spectroscopic methods.					
31377617	0	14	theme	Funnel	116:121	arg1	tubaeformis					103:113	wild Finnish mushroom Craterellus tubaeformis	69:113	wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)	69:134	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	0	14	theme	Funnel	116:121	arg1	Chanterelle					123:133	Funnel Chanterelle	116:133	Funnel Chanterelle	116:133	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	5	15	theme	high-molecular	633:646	arg1	1 → chains					689:698	high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains	633:698	high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains	633:698	The hot water extract consisted mainly of high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains, covalently bound to proteins.					
31377617	7	16	theme	molecular	913:921	arg1	linkages					903:910	the glycosidic linkages	888:910	the glycosidic linkages	888:910	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	16	theme	molecular	913:921	arg1	mass					923:926	molecular mass	913:926	molecular mass	913:926	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	8	17	theme	molecular	1029:1037	arg1	weight					1039:1044	low molecular weight	1025:1044	low molecular weight polysaccharides	1025:1060	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	9	18	with	polysaccharides	1227:1241	arg1	stability					1278:1286	substantially reduced thermal stability	1248:1286	substantially reduced thermal stability	1248:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	2	19	theme	KOH	367:369	arg1	solutions					371:379	KOH solutions	367:379	KOH solutions	367:379	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	0	20	theme	Structural	0:9	arg1	investigation					11:23	Structural investigation	0:23	Structural investigation of cell wall polysaccharides	0:52	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	8	21	theme	low	1025:1027	arg1	weight					1039:1044	low molecular weight	1025:1044	low molecular weight polysaccharides	1025:1060	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	8	22	theme	KOH	994:996	arg1	%					999:999	KOH 2%	994:999	KOH 2%	994:999	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	2	23	theme	tubaeformis	326:336	arg1	bodies					313:318	the fruiting bodies	300:318	the fruiting bodies of C. tubaeformis	300:336	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	0	24	theme	cell	28:31	arg1	polysaccharides					38:52	cell wall polysaccharides	28:52	cell wall polysaccharides	28:52	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	9	25	theme	KOH	1189:1191	arg1	%					1195:1195	KOH 25%	1189:1195	KOH 25%	1189:1195	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	8	26	theme	polysaccharides	1046:1060	arg1	polysaccharides					1046:1060	low molecular weight polysaccharides	1025:1060	low molecular weight polysaccharides	1025:1060	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	8	26	theme	polysaccharides	1046:1060	arg1	amount					1015:1020	the amount	1011:1020	the amount of low molecular weight polysaccharides	1011:1060	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	5	27	theme	hot	595:597	arg1	extract					605:611	The hot water extract	591:611	The hot water extract	591:611	The hot water extract consisted mainly of high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains, covalently bound to proteins.					
31377617	6	28	theme	short → 3	804:812	arg1	branches					792:799	branches	792:799	branches of short → 3	792:812	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	1	29	theme	abundant	200:207	arg1	mushrooms					214:222	the most abundant wild mushrooms	191:222	the most abundant wild mushrooms in Finland	191:233	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	9	30	with	extraction	1173:1182	arg1	%					1195:1195	KOH 25%	1189:1195	KOH 25%	1189:1195	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	8	31	theme	weight	1039:1044	arg1	polysaccharides					1046:1060	low molecular weight polysaccharides	1025:1060	low molecular weight polysaccharides	1025:1060	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	2	32	dep	water	349:353	arg1	solutions					371:379	KOH solutions	367:379	KOH solutions	367:379	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	1	33	theme	wild	209:212	arg1	mushrooms					214:222	the most abundant wild mushrooms	191:222	the most abundant wild mushrooms in Finland	191:233	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	0	34	theme	polysaccharides	38:52	arg1	investigation					11:23	Structural investigation	0:23	Structural investigation of cell wall polysaccharides	0:52	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	6	35	theme	β-Glc	843:847	arg1	residues					849:856	single β-Glc residues	836:856	single β-Glc residues	836:856	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	2	36	theme	polysaccharide	242:255	arg1	fractions					257:265	Three polysaccharide fractions	236:265	Three polysaccharide fractions	236:265	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	0	37	theme	wall	33:36	arg1	polysaccharides					38:52	cell wall polysaccharides	28:52	cell wall polysaccharides	28:52	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	6	38	theme	single	836:841	arg1	residues					849:856	single β-Glc residues	836:856	single β-Glc residues	836:856	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	1	39	from	mushrooms	214:222	arg1	Finland					227:233	Finland	227:233	Finland	227:233	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	3	40	theme	molecular	435:443	arg1	weight					445:450	molecular weight	435:450	molecular weight	435:450	The monomer composition, molecular weight, and chemical structure were determined using chromatographic and spectroscopic methods.					
31377617	5	41	theme	-α-Gal-	681:687	arg1	1 → chains					689:698	high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains	633:698	high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains	633:698	The hot water extract consisted mainly of high-molecular weight → 2,6)-α-Man-(1 → and → 6)-α-Gal-(1 → chains, covalently bound to proteins.					
31377617	9	42	theme	molecular	1210:1218	arg1	weight					1220:1225	low molecular weight	1206:1225	low molecular weight polysaccharides with substantially reduced thermal stability	1206:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	6	43	with	1→	783:784	arg1	branches					792:799	branches	792:799	branches of short → 3	792:812	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	4	44	theme	Thermogravimetric	541:557	arg1	well					585:588	well	585:588	well	585:588	Thermogravimetric analysis was performed as well.					
31377617	4	44	theme	Thermogravimetric	541:557	arg1	analysis					559:566	Thermogravimetric analysis	541:566	Thermogravimetric analysis	541:566	Thermogravimetric analysis was performed as well.					
31377617	9	45	theme	weight	1220:1225	arg1	polysaccharides					1227:1241	low molecular weight polysaccharides	1206:1241	low molecular weight polysaccharides with substantially reduced thermal stability	1206:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	9	46	theme	thermal	1270:1276	arg1	stability					1278:1286	substantially reduced thermal stability	1248:1286	substantially reduced thermal stability	1248:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	9	47	theme	low	1206:1208	arg1	weight					1220:1225	low molecular weight	1206:1225	low molecular weight polysaccharides with substantially reduced thermal stability	1206:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	6	48	theme	-β-Glc-	775:781	arg1	-β-Glc-					814:820	acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-	764:820	acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-	764:820	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	8	49	theme	weight	1094:1099	arg1	distributions					1101:1113	bimodal molecular weight distributions	1076:1113	bimodal molecular weight distributions	1076:1113	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	1	50	theme	Craterellus	137:147	arg1	tubaeformis					149:159	Craterellus tubaeformis	137:159	Craterellus tubaeformis (Funnel Chanterelle)	137:180	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	1	50	theme	Craterellus	137:147	arg1	Chanterelle					169:179	Funnel Chanterelle	162:179	Funnel Chanterelle	162:179	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	7	51	theme	glycosidic	892:901	arg1	linkages					903:910	the glycosidic linkages	888:910	the glycosidic linkages	888:910	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	51	theme	glycosidic	892:901	arg1	mass					923:926	molecular mass	913:926	molecular mass	913:926	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	51	theme	glycosidic	892:901	arg1	stability					940:948	thermal stability	932:948	thermal stability	932:948	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	8	52	theme	molecular	1084:1092	arg1	distributions					1101:1113	bimodal molecular weight distributions	1076:1113	bimodal molecular weight distributions	1076:1113	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	7	53	theme	fractions	972:980	arg1	linkages					903:910	the glycosidic linkages	888:910	the glycosidic linkages	888:910	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	53	theme	fractions	972:980	arg1	mass					923:926	molecular mass	913:926	molecular mass	913:926	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	7	53	theme	fractions	972:980	arg1	stability					940:948	thermal stability	932:948	thermal stability	932:948	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	3	54	theme	chemical	457:464	arg1	structure					466:474	chemical structure	457:474	chemical structure	457:474	The monomer composition, molecular weight, and chemical structure were determined using chromatographic and spectroscopic methods.					
31377617	7	55	theme	alkali	870:875	arg1	use					863:865	The use	859:865	The use of alkali	859:875	The use of alkali influenced the glycosidic linkages, molecular mass and thermal stability of the polysaccharide fractions.					
31377617	2	56	theme	hot	345:347	arg1	water					349:353	hot water	345:353	hot water	345:353	Three polysaccharide fractions were sequentially extracted from the fruiting bodies of C. tubaeformis, using hot water, 2% and 25% KOH solutions, respectively, and purified.					
31377617	0	57	theme	Finnish	74:80	arg1	tubaeformis					103:113	wild Finnish mushroom Craterellus tubaeformis	69:113	wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)	69:134	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	0	57	theme	Finnish	74:80	arg1	Chanterelle					123:133	Funnel Chanterelle	116:133	Funnel Chanterelle	116:133	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	8	58	from	impact	1128:1133	arg1	stability					1150:1158	the thermal stability	1138:1158	the thermal stability	1138:1158	The use of KOH 2% increased the amount of low molecular weight polysaccharides, resulting in bimodal molecular weight distributions, with little impact on the thermal stability.					
31377617	6	59	theme	alkali	735:740	arg1	extracts					742:749	The alkali extracts	731:749	The alkali extracts	731:749	The alkali extracts consisted of acidic → 6)-β-Glc-(1→, with branches of short → 3)-β-Glc-(1 → chains or single β-Glc residues.					
31377617	3	60	theme	chromatographic	498:512	arg1	methods					532:538	chromatographic and spectroscopic methods	498:538	chromatographic and spectroscopic methods	498:538	The monomer composition, molecular weight, and chemical structure were determined using chromatographic and spectroscopic methods.					
31377617	0	61	theme	wild	69:72	arg1	tubaeformis					103:113	wild Finnish mushroom Craterellus tubaeformis	69:113	wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)	69:134	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	0	61	theme	wild	69:72	arg1	Chanterelle					123:133	Funnel Chanterelle	116:133	Funnel Chanterelle	116:133	Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle).					
31377617	9	62	theme	reduced	1262:1268	arg1	stability					1278:1286	substantially reduced thermal stability	1248:1286	substantially reduced thermal stability	1248:1286	Conversely, extraction with KOH 25% provided low molecular weight polysaccharides with substantially reduced thermal stability.					
31377617	1	63	theme	Funnel	162:167	arg1	tubaeformis					149:159	Craterellus tubaeformis	137:159	Craterellus tubaeformis (Funnel Chanterelle)	137:180	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
31377617	1	63	theme	Funnel	162:167	arg1	Chanterelle					169:179	Funnel Chanterelle	162:179	Funnel Chanterelle	162:179	Craterellus tubaeformis (Funnel Chanterelle) is among the most abundant wild mushrooms in Finland.					
30872058	4	0	theme	polysaccharides	549:563	arg1	peaks					540:544	the characteristic absorption peaks	510:544	the characteristic absorption peaks of polysaccharides	510:563	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	5	1	theme	crude	660:664	arg1	UEP					666:668	the crude UEP	656:668	the crude UEP (33.0%)	656:676	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	5	1	theme	crude	660:664	arg1	%					675:675	33.0%	671:675	33.0%	671:675	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	5	2	theme	UEP1-1	598:603	arg1	esterification					580:593	esterification	580:593	esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%)	580:630	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	6	3	theme	23.9	902:905	arg1	%					915:915	23.9 and 35.8%	902:915	%	915:915	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	6	4	theme	%	915:915	arg1	crystallinity					885:897	crystallinity	885:897	crystallinity of 23.9 and 35.8%, respectively	885:929	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	6	5	theme	35.8	911:914	arg1	%					915:915	23.9 and 35.8%	902:915	%	915:915	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	4	6	dep	transform	441:449	arg1	infrared					451:458	infrared	451:458	transform infrared spectrum	441:467	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	0	7	theme	mollissima	109:118	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	7	theme	mollissima	109:118	arg1	Bl					120:121	Castanea mollissima Bl.	100:122	Castanea mollissima Bl.	100:122	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	5	8	theme	UEP2-1	617:622	arg1	esterification					580:593	esterification	580:593	esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%)	580:630	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	6	9	theme	UEP2-1	810:815	arg1	patterns					787:794	The x-ray diffraction patterns	765:794	The x-ray diffraction patterns of UEP1-1 and UEP2-1	765:815	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	4	10	dep	Fourier	433:439	arg1	transform					441:449	transform	441:449	transform infrared spectrum	441:467	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	0	11	theme	Castanea	100:107	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	11	theme	Castanea	100:107	arg1	Bl					120:121	Castanea mollissima Bl.	100:122	Castanea mollissima Bl.	100:122	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	4	12	theme	absorption	529:538	arg1	peaks					540:544	the characteristic absorption peaks	510:544	the characteristic absorption peaks of polysaccharides	510:563	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	7	13	theme	molecular	951:959	arg1	weight					961:966	The weight-average molecular weight	932:966	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1	932:992	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	13	theme	molecular	951:959	arg1	75.4					998:1001	75.4	998:1001	75.4	998:1001	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	13	theme	molecular	951:959	arg1	Mw					969:970	Mw	969:970	Mw	969:970	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	5	14	theme	polysaccharides	721:735	arg1	properties					703:712	the gel properties	695:712	the gel properties of the polysaccharides	695:735	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	4	15	theme	characteristic	514:527	arg1	peaks					540:544	the characteristic absorption peaks	510:544	the characteristic absorption peaks of polysaccharides	510:563	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	3	16	theme	extracted	267:275	arg1	fractions					292:300	The ultrasound extracted polysaccharide fractions	252:300	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1	252:324	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	6	17	theme	UEP1-1	799:804	arg1	patterns					787:794	The x-ray diffraction patterns	765:794	The x-ray diffraction patterns of UEP1-1 and UEP2-1	765:815	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	6	18	theme	crystallinity	885:897	arg1	degree					875:880	a degree	873:880	a degree of crystallinity of 23.9 and 35.8%, respectively	873:929	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	3	19	theme	polysaccharide	277:290	arg1	fractions					292:300	The ultrasound extracted polysaccharide fractions	252:300	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1	252:324	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	6	20	with	polymers	859:866	arg1	degree					875:880	a degree	873:880	a degree of crystallinity of 23.9 and 35.8%, respectively	873:929	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	2	21	theme	present	137:143	arg1	paper					145:149	The present paper	133:149	The present paper	133:149	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	3	22	theme	successive	343:352	arg1	ultrasound-assisted-extraction					354:383	successive ultrasound-assisted-extraction	343:383	successive ultrasound-assisted-extraction	343:383	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	2	23	from	kernel	244:249	arg1	polysaccharides					171:185	the polysaccharides	167:185	the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel	167:249	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	8	24	with	glucose	1087:1093	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	9	25	theme	tyrosinase	1240:1249	arg1	activity					1251:1258	tyrosinase activity	1240:1258	tyrosinase activity	1240:1258	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	3	26	theme	ultrasound	256:265	arg1	fractions					292:300	The ultrasound extracted polysaccharide fractions	252:300	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1	252:324	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	6	27	theme	diffraction	775:785	arg1	patterns					787:794	The x-ray diffraction patterns	765:794	The x-ray diffraction patterns of UEP1-1 and UEP2-1	765:815	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	8	28	theme	monosaccharide	1035:1048	arg1	composition					1050:1060	The monosaccharide composition	1031:1060	The monosaccharide composition of UEP1-1 and UEP2-1	1031:1081	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	8	28	theme	monosaccharide	1035:1048	arg1	glucose					1087:1093	glucose	1087:1093	glucose	1087:1093	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	9	29	theme	inhibitory	1219:1228	arg1	effect					1230:1235	a dose-dependent inhibitory effect	1202:1235	a dose-dependent inhibitory effect on tyrosinase activity	1202:1258	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	3	30	theme	G-100	407:411	arg1	chromatography					413:426	Sephadex G-100 chromatography	398:426	Sephadex G-100 chromatography	398:426	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	6	31	theme	x-ray	769:773	arg1	patterns					787:794	The x-ray diffraction patterns	765:794	The x-ray diffraction patterns of UEP1-1 and UEP2-1	765:815	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	8	32	with	arabinose	1107:1115	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	4	33	contain	had	506:508	arg1	UEP2-1					499:504	UEP2-1	499:504	UEP2-1	499:504	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	4	33	contain	had	506:508	arg1	UEP1-1					488:493	UEP1-1	488:493	UEP1-1	488:493	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	4	33	contain	had	506:508	arg2	peaks					540:544	the characteristic absorption peaks	510:544	the characteristic absorption peaks of polysaccharides	510:563	The Fourier transform infrared spectrum revealed that both UEP1-1 and UEP2-1 had the characteristic absorption peaks of polysaccharides.					
30872058	8	34	theme	different	1162:1170	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	8	35	theme	UEP1-1	1065:1070	arg1	composition					1050:1060	The monosaccharide composition	1031:1060	The monosaccharide composition of UEP1-1 and UEP2-1	1031:1081	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	8	35	theme	UEP1-1	1065:1070	arg1	glucose					1087:1093	glucose	1087:1093	glucose	1087:1093	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	9	36	theme	dose-dependent	1204:1217	arg1	effect					1230:1235	a dose-dependent inhibitory effect	1202:1235	a dose-dependent inhibitory effect on tyrosinase activity	1202:1258	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	0	37	theme	inhibition	46:55	arg1	activity					57:64	tyrosinase inhibition activity	35:64	tyrosinase inhibition activity	35:64	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	5	38	theme	gel	699:701	arg1	properties					703:712	the gel properties	695:712	the gel properties of the polysaccharides	695:735	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	0	39	from	chestnut	90:97	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	39	from	chestnut	90:97	arg1	Bl					120:121	Castanea mollissima Bl.	100:122	Castanea mollissima Bl.	100:122	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	39	from	chestnut	90:97	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	39	from	chestnut	90:97	arg1	activity					57:64	tyrosinase inhibition activity	35:64	tyrosinase inhibition activity	35:64	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	9	40	theme	inhibition	1269:1278	arg1	mode					1280:1283	the inhibition mode	1265:1283	the inhibition mode	1265:1283	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	9	40	theme	inhibition	1269:1278	arg1	competitive					1301:1311	competitive	1301:1311	competitive	1301:1311	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	0	41	theme	tyrosinase	35:44	arg1	activity					57:64	tyrosinase inhibition activity	35:64	tyrosinase inhibition activity	35:64	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	8	42	theme	UEP2-1	1076:1081	arg1	composition					1050:1060	The monosaccharide composition	1031:1060	The monosaccharide composition of UEP1-1 and UEP2-1	1031:1081	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	8	42	theme	UEP2-1	1076:1081	arg1	glucose					1087:1093	glucose	1087:1093	glucose	1087:1093	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	9	43	from	effect	1230:1235	arg1	activity					1251:1258	tyrosinase activity	1240:1258	tyrosinase activity	1240:1258	UEP1-1 displayed a dose-dependent inhibitory effect on tyrosinase activity, and the inhibition mode was found to be competitive.					
30872058	2	44	theme	chestnut	235:242	arg1	kernel					244:249	chestnut kernel	235:249	chestnut kernel	235:249	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	7	45	theme	weight-average	936:949	arg1	weight					961:966	The weight-average molecular weight	932:966	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1	932:992	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	45	theme	weight-average	936:949	arg1	75.4					998:1001	75.4	998:1001	75.4	998:1001	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	45	theme	weight-average	936:949	arg1	Mw					969:970	Mw	969:970	Mw	969:970	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	6	46	theme	semi-crystalline	842:857	arg1	polymers					859:866	semi-crystalline polymers	842:866	semi-crystalline polymers	842:866	The x-ray diffraction patterns of UEP1-1 and UEP2-1 suggested that they were semi-crystalline polymers with a degree of crystallinity of 23.9 and 35.8%, respectively.					
30872058	7	47	theme	UEP2-1	987:992	arg1	weight					961:966	The weight-average molecular weight	932:966	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1	932:992	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	47	theme	UEP2-1	987:992	arg1	75.4					998:1001	75.4	998:1001	75.4	998:1001	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	47	theme	UEP2-1	987:992	arg1	Mw					969:970	Mw	969:970	Mw	969:970	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	8	48	with	xylose	1127:1132	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	2	49	theme	inhibitory	210:219	arg1	activity					221:228	potent tyrosinase inhibitory activity	192:228	potent tyrosinase inhibitory activity	192:228	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	8	50	with	mannose	1118:1124	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	8	51	with	fructose	1148:1155	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	2	52	theme	tyrosinase	199:208	arg1	activity					221:228	potent tyrosinase inhibitory activity	192:228	potent tyrosinase inhibitory activity	192:228	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	7	53	theme	UEP1-1	976:981	arg1	weight					961:966	The weight-average molecular weight	932:966	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1	932:992	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	53	theme	UEP1-1	976:981	arg1	75.4					998:1001	75.4	998:1001	75.4	998:1001	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	7	53	theme	UEP1-1	976:981	arg1	Mw					969:970	Mw	969:970	Mw	969:970	The weight-average molecular weight (Mw) of UEP1-1 and UEP2-1 was 75.4 and 59.9 kDA, respectively.					
30872058	2	54	with	polysaccharides	171:185	arg1	activity					221:228	potent tyrosinase inhibitory activity	192:228	potent tyrosinase inhibitory activity	192:228	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	8	55	with	rhamnose	1135:1142	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	3	56	dep	fractions	292:300	arg1	UEP1-1					307:312	UEP1-1	307:312	UEP1-1	307:312	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	3	56	dep	fractions	292:300	arg1	UEP2-1					319:324	UEP2-1	319:324	UEP2-1	319:324	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	3	56	dep	fractions	292:300	arg1	1-1					302:304	1-1	302:304	1-1 (UEP1-1)	302:313	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
30872058	2	57	theme	potent	192:197	arg1	activity					221:228	potent tyrosinase inhibitory activity	192:228	potent tyrosinase inhibitory activity	192:228	The present paper aimed to obtain the polysaccharides with potent tyrosinase inhibitory activity from chestnut kernel.					
30872058	5	58	theme	esterification	580:593	arg1	higher					636:641	higher	636:641	higher	636:641	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	5	58	theme	esterification	580:593	arg1	degree					570:575	The degree	566:575	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%)	566:630	The degree of esterification of UEP1-1 (52.4%) and UEP2-1 (49.0%) was higher than that of the crude UEP (33.0%), indicating that the gel properties of the polysaccharides changed after purification.					
30872058	0	59	theme	polysaccharides	69:83	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	59	theme	polysaccharides	69:83	arg1	Bl					120:121	Castanea mollissima Bl.	100:122	Castanea mollissima Bl.	100:122	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	59	theme	polysaccharides	69:83	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	0	59	theme	polysaccharides	69:83	arg1	activity					57:64	tyrosinase inhibition activity	35:64	tyrosinase inhibition activity	35:64	Purification, characterization and tyrosinase inhibition activity of polysaccharides from chestnut (Castanea mollissima Bl.)					
30872058	8	60	with	galactose	1096:1104	arg1	proportions					1172:1182	different proportions	1162:1182	different proportions	1162:1182	The monosaccharide composition of UEP1-1 and UEP2-1 was glucose, galactose, arabinose, mannose, xylose, rhamnose and fructose with different proportions.					
30872058	3	61	theme	Sephadex	398:405	arg1	chromatography					413:426	Sephadex G-100 chromatography	398:426	Sephadex G-100 chromatography	398:426	The ultrasound extracted polysaccharide fractions 1-1 (UEP1-1) and UEP2-1 were obtained by successive ultrasound-assisted-extraction, DEAE-52 and Sephadex G-100 chromatography.					
29949745	2	0	theme	electron	508:515	arg1	microscopy					517:526	scanning electron microscopy	499:526	scanning electron microscopy (SEM)	499:532	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	0	theme	electron	508:515	arg1	SEM					529:531	SEM	529:531	SEM	529:531	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	7	1	theme	hydrogel	1075:1082	arg1	film					1084:1087	The hydrogel film	1071:1087	The hydrogel film	1071:1087	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	7	2	contain	had	1089:1091	arg1	film					1084:1087	The hydrogel film	1071:1087	The hydrogel film	1071:1087	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	7	2	contain	had	1089:1091	arg2	strength					1106:1113	good tensile strength	1093:1113	good tensile strength of 19.1-31.0 MPa and elongation	1093:1145	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	1	3	theme	novel	154:158	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	1	4	theme	ratio	268:272	arg1	composition					274:284	different weight ratio composition	251:284	different weight ratio composition	251:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	2	5	theme	scanning	499:506	arg1	microscopy					517:526	scanning electron microscopy	499:526	scanning electron microscopy (SEM)	499:532	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	5	theme	scanning	499:506	arg1	SEM					529:531	SEM	529:531	SEM	529:531	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	1	6	theme	synergistic	160:170	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	5	7	theme	weight	896:901	arg1	loss					903:906	5% weight loss	893:906	5% weight loss (T5%)	893:912	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	5	7	theme	weight	896:901	arg1	%					911:911	T5%	909:911	T5%	909:911	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	0	8	theme	water	106:110	arg1	properties					120:129	water barrier properties	106:129	water barrier properties	106:129	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	6	9	dep	1.8-2.4	986:992	arg1	range					1020:1024	the range	1016:1024	the range of 32-65.8° and moisture content 16.5-21.51	1016:1068	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	4	10	theme	contact	816:822	arg1	WCA					831:833	WCA	831:833	WCA	831:833	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	10	theme	contact	816:822	arg1	angle					824:828	water contact angle	810:828	water contact angle (WCA)	810:834	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	6	11	theme	moisture	1042:1049	arg1	content					1051:1057	moisture content 16.5-21.51	1042:1068	moisture content 16.5-21.51	1042:1068	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	1	12	theme	films	366:370	arg1	properties					348:357	the physical properties	335:357	the physical properties of the films	335:370	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	4	13	theme	water	810:814	arg1	WCA					831:833	WCA	831:833	WCA	831:833	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	13	theme	water	810:814	arg1	angle					824:828	water contact angle	810:828	water contact angle (WCA)	810:834	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	5	14	theme	5	893:893	arg1	%					894:894	%	894:894	%	894:894	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	7	15	theme	elongation	1136:1145	arg1	strength					1106:1113	good tensile strength	1093:1113	good tensile strength of 19.1-31.0 MPa and elongation	1093:1145	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	1	16	from	effect	303:308	arg1	properties					348:357	the physical properties	335:357	the physical properties of the films	335:370	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	9	17	theme	films	1320:1324	arg1	properties					1277:1286	properties	1277:1286	properties of the ternary k-C/X/G hydrogel films	1277:1324	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	2	18	dep	structure	377:385	arg1	The					373:375	The	373:375	The	373:375	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	19	theme	morphological	391:403	arg1	properties					405:414	morphological properties	391:414	morphological properties	391:414	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	1	20	theme	transparent	172:182	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	0	21	theme	barrier	112:118	arg1	properties					120:129	water barrier properties	106:129	water barrier properties	106:129	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	4	22	theme	vapor	784:788	arg1	WVP					804:806	WVP	804:806	WVP	804:806	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	22	theme	vapor	784:788	arg1	permeability					790:801	water vapor permeability	778:801	water vapor permeability (WVP)	778:807	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	2	23	theme	infrared	468:475	arg1	FT-IR					491:495	FT-IR	491:495	FT-IR	491:495	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	23	theme	infrared	468:475	arg1	spectroscopy					477:488	Fourier-transform infrared spectroscopy	450:488	Fourier-transform infrared spectroscopy (FT-IR)	450:496	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	5	24	from	loss	903:906	arg1	temperature					878:888	The temperature	874:888	The temperature at 5% weight loss (T5%)	874:912	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	1	25	theme	k-Carrageenan/Xanthan	184:204	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	0	26	theme	synergistic	6:16	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	9	27	theme	linking	1384:1390	arg1	cross					1378:1382	cross linking	1378:1390	cross linking within the blends	1378:1408	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	1	28	theme	gum/Gellan	206:215	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	0	29	theme	Novel	0:4	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	3	30	theme	new	703:705	arg1	material					707:714	a new material	701:714	a new material	701:714	Results for FT-IR, DSC and SEM analysis showed a clear interaction between k-C, X, and G to form a new material.					
29949745	1	31	theme	gum	217:219	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	0	32	theme	k-Carrageenan/Xanthan	30:50	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	5	33	theme	T5	909:910	arg1	loss					903:906	5% weight loss	893:906	5% weight loss (T5%)	893:912	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	5	33	theme	T5	909:910	arg1	%					911:911	T5%	909:911	T5%	909:911	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	4	34	theme	moisture	840:847	arg1	content					849:855	moisture content	840:855	moisture content	840:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	9	35	theme	ternary	1295:1301	arg1	films					1320:1324	the ternary k-C/X/G hydrogel films	1291:1324	the ternary k-C/X/G hydrogel films	1291:1324	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	2	36	theme	X-ray	535:539	arg1	XRD					554:556	XRD	554:556	XRD	554:556	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	36	theme	X-ray	535:539	arg1	diffraction					541:551	X-ray diffraction	535:551	X-ray diffraction (XRD)	535:557	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	0	37	theme	transparent	18:28	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	5	38	theme	%	894:894	arg1	loss					903:906	5% weight loss	893:906	5% weight loss (T5%)	893:912	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	5	38	theme	%	894:894	arg1	%					911:911	T5%	909:911	T5%	909:911	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	3	39	theme	SEM	631:633	arg1	analysis					635:642	SEM analysis	631:642	SEM analysis	631:642	Results for FT-IR, DSC and SEM analysis showed a clear interaction between k-C, X, and G to form a new material.					
29949745	7	40	theme	tensile	1170:1176	arg1	modulus					1178:1184	tensile modulus	1170:1184	tensile modulus	1170:1184	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	9	41	theme	properties	1277:1286	arg1	range					1268:1272	The range	1264:1272	The range of properties of the ternary k-C/X/G hydrogel films	1264:1324	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	1	42	theme	k-C/X/G	222:228	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	0	43	theme	gum	63:65	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	9	44	theme	presence	1343:1350	arg1	interaction					1362:1372	the presence molecular interaction	1339:1372	the presence molecular interaction	1339:1372	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	0	45	dep	film	76:79	arg1	thermal					94:100	thermal	94:100	thermal	94:100	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	0	45	dep	film	76:79	arg1	Mechanical					82:91	Mechanical	82:91	Mechanical	82:91	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	0	45	dep	film	76:79	arg1	properties					120:129	water barrier properties	106:129	water barrier properties	106:129	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	9	46	theme	k-C/X/G	1303:1309	arg1	films					1320:1324	the ternary k-C/X/G hydrogel films	1291:1324	the ternary k-C/X/G hydrogel films	1291:1324	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	2	47	theme	films	423:427	arg1	properties					405:414	morphological properties	391:414	morphological properties	391:414	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	47	theme	films	423:427	arg1	structure					377:385	structure	377:385	structure	377:385	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	0	48	theme	gum/Gellan	52:61	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	2	49	theme	Fourier-transform	450:466	arg1	FT-IR					491:495	FT-IR	491:495	FT-IR	491:495	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	49	theme	Fourier-transform	450:466	arg1	spectroscopy					477:488	Fourier-transform infrared spectroscopy	450:488	Fourier-transform infrared spectroscopy (FT-IR)	450:496	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	1	50	theme	hydrogel	231:238	arg1	films					240:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films	154:244	novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition	154:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	1	51	theme	compositions	319:330	arg1	effect					303:308	the effect	299:308	the effect of these compositions on the physical properties of the films	299:370	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	4	52	theme	water	778:782	arg1	WVP					804:806	WVP	804:806	WVP	804:806	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	52	theme	water	778:782	arg1	permeability					790:801	water vapor permeability	778:801	water vapor permeability (WVP)	778:807	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	0	53	theme	hydrogel	67:74	arg1	film					76:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film	0:79	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.	0:130	Novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum hydrogel film: Mechanical, thermal and water barrier properties.					
29949745	2	54	theme	scanning	576:583	arg1	DSC					598:600	DSC	598:600	DSC	598:600	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	54	theme	scanning	576:583	arg1	calorimeter					585:595	differential scanning calorimeter	563:595	differential scanning calorimeter (DSC)	563:601	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	3	55	theme	clear	653:657	arg1	interaction					659:669	a clear interaction	651:669	a clear interaction between k-C, X, and G to form a new material	651:714	Results for FT-IR, DSC and SEM analysis showed a clear interaction between k-C, X, and G to form a new material.					
29949745	7	56	theme	1.6-2.4 GPa	1189:1199	arg1	%					1164:1164	13-19%	1159:1164	13-19%	1159:1164	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	7	56	theme	1.6-2.4 GPa	1189:1199	arg1	modulus					1178:1184	tensile modulus	1170:1184	tensile modulus	1170:1184	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	7	56	theme	1.6-2.4 GPa	1189:1199	arg1	1.6-2.4 GPa					1189:1199	1.6-2.4 GPa	1189:1199	1.6-2.4 GPa	1189:1199	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	6	57	theme	contact	995:1001	arg1	1.8-2.4					986:992	1.8-2.4	986:992	1.8-2.4	986:992	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	6	57	theme	contact	995:1001	arg1	angle					1003:1007	contact angle	995:1007	contact angle	995:1007	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	6	58	theme	content	1051:1057	arg1	range					1020:1024	the range	1016:1024	the range of 32-65.8° and moisture content 16.5-21.51	1016:1068	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	2	59	theme	differential	563:574	arg1	DSC					598:600	DSC	598:600	DSC	598:600	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	2	59	theme	differential	563:574	arg1	calorimeter					585:595	differential scanning calorimeter	563:595	differential scanning calorimeter (DSC)	563:601	The structure and morphological properties of the films were investigated by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimeter (DSC).					
29949745	9	60	dep	suggest	1326:1332	arg1	cross					1378:1382	cross linking	1378:1390	cross linking within the blends	1378:1408	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	9	60	dep	suggest	1326:1332	arg1	interaction					1362:1372	the presence molecular interaction	1339:1372	the presence molecular interaction	1339:1372	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	7	61	dep	%	1164:1164	arg1	break					1150:1154	break	1150:1154	break	1150:1154	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	8	62	theme	UV	1206:1207	arg1	results					1209:1215	The UV results	1202:1215	The UV results	1202:1215	The UV results indicate that the films were very transparent.					
29949745	4	63	theme	mechanical	721:730	arg1	angle					824:828	water contact angle	810:828	water contact angle (WCA)	810:834	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	63	theme	mechanical	721:730	arg1	content					849:855	moisture content	840:855	moisture content	840:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	63	theme	mechanical	721:730	arg1	permeability					790:801	water vapor permeability	778:801	water vapor permeability (WVP)	778:807	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	63	theme	mechanical	721:730	arg1	properties					759:768	The mechanical, thermal and water barrier properties	717:768	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content	717:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	5	64	theme	64.2-121.9 °C	934:946	arg1	range					925:929	the range	921:929	the range of 64.2-121.9 °C	921:946	The temperature at 5% weight loss (T5%) are in the range of 64.2-121.9 °C.					
29949745	4	65	theme	thermal	733:739	arg1	angle					824:828	water contact angle	810:828	water contact angle (WCA)	810:834	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	65	theme	thermal	733:739	arg1	content					849:855	moisture content	840:855	moisture content	840:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	65	theme	thermal	733:739	arg1	permeability					790:801	water vapor permeability	778:801	water vapor permeability (WVP)	778:807	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	65	theme	thermal	733:739	arg1	properties					759:768	The mechanical, thermal and water barrier properties	717:768	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content	717:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	6	66	theme	1.8-2.4	986:992	arg1	range					977:981	the range	973:981	the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51	973:1068	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	9	67	theme	molecular	1352:1360	arg1	interaction					1362:1372	the presence molecular interaction	1339:1372	the presence molecular interaction	1339:1372	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	4	68	theme	barrier	751:757	arg1	angle					824:828	water contact angle	810:828	water contact angle (WCA)	810:834	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	68	theme	barrier	751:757	arg1	content					849:855	moisture content	840:855	moisture content	840:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	68	theme	barrier	751:757	arg1	permeability					790:801	water vapor permeability	778:801	water vapor permeability (WVP)	778:807	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	4	68	theme	barrier	751:757	arg1	properties					759:768	The mechanical, thermal and water barrier properties	717:768	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content	717:855	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	9	69	theme	hydrogel	1311:1318	arg1	films					1320:1324	the ternary k-C/X/G hydrogel films	1291:1324	the ternary k-C/X/G hydrogel films	1291:1324	The range of properties of the ternary k-C/X/G hydrogel films suggest that the presence molecular interaction and cross linking within the blends.					
29949745	6	70	theme	32-65.8°	1029:1036	arg1	range					1020:1024	the range	1016:1024	the range of 32-65.8° and moisture content 16.5-21.51	1016:1068	The WVP exhibits are in the range of 1.8-2.4, contact angle are in the range of 32-65.8° and moisture content 16.5-21.51.					
29949745	1	71	theme	different	251:259	arg1	composition					274:284	different weight ratio composition	251:284	different weight ratio composition	251:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	7	72	theme	good	1093:1096	arg1	strength					1106:1113	good tensile strength	1093:1113	good tensile strength of 19.1-31.0 MPa and elongation	1093:1145	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	1	73	theme	physical	339:346	arg1	properties					348:357	the physical properties	335:357	the physical properties of the films	335:370	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	4	74	theme	water	745:749	arg1	barrier					751:757	water barrier	745:757	water barrier	745:757	The mechanical, thermal and water barrier properties such as water vapor permeability (WVP), water contact angle (WCA) and moisture content were determined.					
29949745	1	75	with	films	240:244	arg1	composition					274:284	different weight ratio composition	251:284	different weight ratio composition	251:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
29949745	7	76	theme	19.1-31.0 MPa	1118:1130	arg1	strength					1106:1113	good tensile strength	1093:1113	good tensile strength of 19.1-31.0 MPa and elongation	1093:1145	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	7	77	theme	tensile	1098:1104	arg1	strength					1106:1113	good tensile strength	1093:1113	good tensile strength of 19.1-31.0 MPa and elongation	1093:1145	The hydrogel film had good tensile strength of 19.1-31.0 MPa and elongation at break of 13-19% and tensile modulus of 1.6-2.4 GPa.					
29949745	1	78	theme	weight	261:266	arg1	composition					274:284	different weight ratio composition	251:284	different weight ratio composition	251:284	The aim is to develop novel synergistic transparent k-Carrageenan/Xanthan gum/Gellan gum (k-C/X/G) hydrogel films with different weight ratio composition and to study the effect of these compositions on the physical properties of the films.					
30446091	2	0	theme	microscopy	410:419	arg1	images					421:426	Transmission electron microscopy images	388:426	Transmission electron microscopy images	388:426	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	2	1	theme	electron	401:408	arg1	microscopy					410:419	Transmission electron microscopy	388:419	Transmission electron microscopy images	388:426	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	1	2	theme	hydroxyapatite	307:320	arg1	stability					275:283	colloidal stability	265:283	colloidal stability	265:283	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	1	2	theme	hydroxyapatite	307:320	arg1	dispersion					293:302	the dispersion	289:302	the dispersion of hydroxyapatite (HA)	289:325	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	5	3	from	effect	877:882	arg1	composites					901:910	the BHC composites	893:910	the BHC composites with a decreased CrI%	893:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	5	4	theme	HA	887:888	arg1	effect					877:882	the effect	873:882	the effect of HA on the BHC composites with a decreased CrI%	873:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	5	5	theme	thermal	844:850	arg1	degradation					852:862	thermal degradation	844:862	thermal degradation	844:862	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	2	6	theme	1.66	550:553	arg1	ratio					541:545	a Ca/P ratio	534:545	a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA	534:600	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	5	7	with	composites	901:910	arg1	%					932:932	a decreased CrI%	917:932	a decreased CrI%	917:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	6	8	theme	cell	1066:1069	arg1	viability					1071:1079	cell viability	1066:1079	cell viability	1066:1079	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	5	9	theme	thermal	947:953	arg1	property					955:962	thermal property	947:962	thermal property	947:962	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	1	10	theme	cellulose	224:232	arg1	CNCs					248:251	CNCs	248:251	CNCs	248:251	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	1	10	theme	cellulose	224:232	arg1	nanocrystals					234:245	cellulose nanocrystals	224:245	cellulose nanocrystals (CNCs)	224:252	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	1	11	theme	Bacterial	101:109	arg1	nanocrystal					150:160	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal	101:160	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites	101:177	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	1	11	theme	Bacterial	101:109	arg1	BHC					163:165	BHC	163:165	BHC	163:165	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	2	12	theme	spectroscopy	450:461	arg1	results					469:475	energy dispersive spectroscopy (EDS) results	432:475	energy dispersive spectroscopy (EDS) results	432:475	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	2	13	theme	HA	505:506	arg1	dispersion					491:500	the dispersion	487:500	the dispersion of HA on the CNC particles	487:527	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	1	14	theme	nanocellulose/hydroxyapatite/cellulose	111:148	arg1	nanocrystal					150:160	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal	101:160	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites	101:177	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	1	14	theme	nanocellulose/hydroxyapatite/cellulose	111:148	arg1	BHC					163:165	BHC	163:165	BHC	163:165	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	2	15	theme	dispersive	439:448	arg1	spectroscopy					450:461	energy dispersive spectroscopy	432:461	energy dispersive spectroscopy (EDS) results	432:475	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	2	15	theme	dispersive	439:448	arg1	EDS					464:466	EDS	464:466	EDS	464:466	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	3	16	dep	assistance	700:709	arg1	BHA					712:714	BHA (0.25	712:720	BHA (0.25	712:720	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	1	17	theme	nanocrystal	150:160	arg1	composites					168:177	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites	101:177	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites	101:177	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	2	18	theme	energy	432:437	arg1	spectroscopy					450:461	energy dispersive spectroscopy	432:461	energy dispersive spectroscopy (EDS) results	432:475	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	2	18	theme	energy	432:437	arg1	EDS					464:466	EDS	464:466	EDS	464:466	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	0	19	theme	nanocellulose	39:51	arg1	scaffold					53:60	hydroxyapatite-bacterial nanocellulose scaffold	14:60	hydroxyapatite-bacterial nanocellulose scaffold	14:60	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.					
30446091	2	20	theme	stoichiometric	584:597	arg1	HA					599:600	the stoichiometric HA	580:600	the stoichiometric HA	580:600	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	5	21	theme	CrI	929:931	arg1	%					932:932	a decreased CrI%	917:932	a decreased CrI%	917:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	3	22	dep	showed	634:639	arg1	integration					650:660	the integration	646:660	the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt	646:731	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	0	23	theme	hydroxyapatite-bacterial	14:37	arg1	scaffold					53:60	hydroxyapatite-bacterial nanocellulose scaffold	14:60	hydroxyapatite-bacterial nanocellulose scaffold	14:60	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.					
30446091	5	24	theme	decreased	919:927	arg1	%					932:932	a decreased CrI%	917:932	a decreased CrI%	917:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	3	25	theme	HA	669:670	arg1	integration					650:660	the integration	646:660	the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt	646:731	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	6	26	theme	negative	1115:1122	arg1	control					1124:1130	the negative control	1111:1130	the negative control (99.2 ± 0.08%)	1111:1145	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	6	26	theme	negative	1115:1122	arg1	%					1144:1144	99.2 ± 0.08%	1133:1144	99.2 ± 0.08%	1133:1144	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	6	27	theme	bone	1028:1031	arg1	engineering					1040:1050	bone tissue engineering	1028:1050	bone tissue engineering scaffold	1028:1059	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	1	28	theme	bacterial	338:346	arg1	period					380:385	the bacterial nanocellulose (BNC) cultivation period	334:385	the bacterial nanocellulose (BNC) cultivation period	334:385	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	3	29	theme	EDS	622:624	arg1	results					626:632	EDS results	622:632	EDS results	622:632	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	6	30	theme	engineering	1040:1050	arg1	scaffold					1052:1059	bone tissue engineering scaffold	1028:1059	bone tissue engineering scaffold	1028:1059	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	3	31	theme	SEM	607:609	arg1	images					611:616	The SEM images	603:616	The SEM images	603:616	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	2	32	theme	CNC	515:517	arg1	particles					519:527	the CNC particles	511:527	the CNC particles	511:527	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	1	33	theme	nanocellulose	348:360	arg1	period					380:385	the bacterial nanocellulose (BNC) cultivation period	334:385	the bacterial nanocellulose (BNC) cultivation period	334:385	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	0	34	theme	scaffold	53:60	arg1	Production					0:9	Production	0:9	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.	0:99	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.					
30446091	5	35	theme	Fourier-transform	799:815	arg1	analysis					826:833	Fourier-transform infrared analysis	799:833	Fourier-transform infrared analysis	799:833	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	1	36	theme	colloidal	265:273	arg1	stability					275:283	colloidal stability	265:283	colloidal stability	265:283	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	2	37	theme	Ca/P	536:539	arg1	ratio					541:545	a Ca/P ratio	534:545	a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA	534:600	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	2	38	theme	Transmission	388:399	arg1	microscopy					410:419	Transmission electron microscopy	388:419	Transmission electron microscopy images	388:426	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	1	39	theme	BNC	363:365	arg1	period					380:385	the bacterial nanocellulose (BNC) cultivation period	334:385	the bacterial nanocellulose (BNC) cultivation period	334:385	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	2	40	from	dispersion	491:500	arg1	particles					519:527	the CNC particles	511:527	the CNC particles	511:527	Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA.					
30446091	6	41	with	composites	1013:1022	arg1	viability					1071:1079	cell viability	1066:1079	cell viability	1066:1079	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	5	42	theme	BHC	897:899	arg1	composites					901:910	the BHC composites	893:910	the BHC composites with a decreased CrI%	893:932	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	3	43	theme	CNC	696:698	arg1	assistance					700:709	CNC assistance	696:709	CNC assistance (BHA (0.25	696:720	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	0	44	with	Production	0:9	arg1	assist					67:72	assist	67:72	assist	67:72	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.					
30446091	6	45	theme	BHC	1009:1011	arg1	composites					1013:1022	BHC composites	1009:1022	BHC composites for bone tissue engineering scaffold with cell viability	1009:1079	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	3	46	theme	BNC	676:678	arg1	network					680:686	BNC network	676:686	BNC network	676:686	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	4	47	dep	%	733:733	arg1	composites					736:745	composites	736:745	composites	736:745	%) composites) was less than that for BHC at both concentrations.					
30446091	3	48	theme	network	680:686	arg1	integration					650:660	the integration	646:660	the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt	646:731	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	6	49	theme	tissue	1033:1038	arg1	engineering					1040:1050	bone tissue engineering	1028:1050	bone tissue engineering scaffold	1028:1059	Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).					
30446091	3	50	dep	HA	669:670	arg1	the					665:667	the	665:667	the	665:667	The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.					
30446091	0	51	theme	cellulose	77:85	arg1	nanocrystals					87:98	cellulose nanocrystals	77:98	cellulose nanocrystals	77:98	Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.					
30446091	1	52	theme	cultivation	368:378	arg1	period					380:385	the bacterial nanocellulose (BNC) cultivation period	334:385	the bacterial nanocellulose (BNC) cultivation period	334:385	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
30446091	5	53	theme	infrared	817:824	arg1	analysis					826:833	Fourier-transform infrared analysis	799:833	Fourier-transform infrared analysis	799:833	Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property.					
30446091	1	54	theme	in-situ	200:206	arg1	synthesis					208:216	in-situ synthesis	200:216	in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period	200:385	Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period.					
29519545	1	0	theme	dynamic	88:94	arg1	characterization					125:140	A dynamic, rheological, and structural characterization	86:140	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	86:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	1	theme	gel-like	153:160	arg1	systems					162:168	aqueous gel-like systems	145:168	aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	145:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	7	2	theme	layer	1380:1384	arg1	removal					1362:1368	the removal	1358:1368	the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996	1358:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	3	3	theme	activation	601:610	arg1	energy					612:617	the activation energy	597:617	the activation energy related to the relaxation process of the system	597:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	1	4	theme	systems	162:168	arg1	characterization					125:140	A dynamic, rheological, and structural characterization	86:140	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	86:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	5	5	theme	components	1018:1027	arg1	role					998:1001	the role	994:1001	the role of the various components on the mesh size of the network	994:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	6	6	theme	applicative	1157:1167	arg1	purposes					1169:1176	applicative purposes	1157:1176	applicative purposes	1157:1176	The structural and mechanical characteristics of these systems suggest their potential use for applicative purposes.					
29519545	7	7	theme	XIX	1284:1286	arg1	fragment					1295:1302	a XIX stucco fragment	1282:1302	a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996	1282:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	1	8	theme	rheological	97:107	arg1	characterization					125:140	A dynamic, rheological, and structural characterization	86:140	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	86:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	2	9	from	plasticizer	305:315	arg1	formulation					324:334	the formulation	320:334	the formulation	320:334	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	7	10	theme	stucco	1288:1293	arg1	fragment					1295:1302	a XIX stucco fragment	1282:1302	a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996	1282:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	11	theme	dirt	1375:1378	arg1	layer					1380:1384	a dirt layer	1373:1384	a dirt layer composed by dust	1373:1401	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	2	12	theme	network	451:457	arg1	formation					459:467	the gel network formation	443:467	the gel network formation	443:467	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	7	13	theme	matter	1420:1425	arg1	removal					1362:1368	the removal	1358:1368	the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996	1358:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	3	14	from	effect	535:540	arg1	energy					612:617	the activation energy	597:617	the activation energy related to the relaxation process of the system	597:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	3	14	from	effect	535:540	arg1	behaviour					580:588	the rheological behaviour	564:588	the rheological behaviour	564:588	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	2	15	theme	gel	447:449	arg1	formation					459:467	the gel network formation	443:467	the gel network formation	443:467	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	7	16	theme	fragment	1295:1302	arg1	surface					1271:1277	the surface	1267:1277	the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996	1267:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	6	17	theme	potential	1139:1147	arg1	use					1149:1151	their potential use	1133:1151	their potential use for applicative purposes	1133:1176	The structural and mechanical characteristics of these systems suggest their potential use for applicative purposes.					
29519545	5	18	theme	structural	879:888	arg1	characterisation					890:905	a structural characterisation	877:905	a structural characterisation	877:905	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	1	19	theme	hydroxypropyl	181:193	arg1	glycerol					221:228	glycerol	221:228	glycerol	221:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	19	theme	hydroxypropyl	181:193	arg1	HPG					205:207	HPG	205:207	HPG	205:207	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	19	theme	hydroxypropyl	181:193	arg1	borax					211:215	borax	211:215	borax	211:215	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	19	theme	hydroxypropyl	181:193	arg1	gum					200:202	hydroxypropyl guar gum	181:202	hydroxypropyl guar gum (HPG)	181:208	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	3	20	theme	relaxation	634:643	arg1	process					645:651	the relaxation process	630:651	the relaxation process of the system	630:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	4	21	theme	gels	771:774	arg1	properties					751:760	the mechanical properties	736:760	the mechanical properties of these gels	736:774	The results obtained suggest that the mechanical properties of these gels can be tuned and controlled by modulating the formulation in a wide range of compositions.					
29519545	1	22	theme	guar	195:198	arg1	glycerol					221:228	glycerol	221:228	glycerol	221:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	22	theme	guar	195:198	arg1	HPG					205:207	HPG	205:207	HPG	205:207	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	22	theme	guar	195:198	arg1	borax					211:215	borax	211:215	borax	211:215	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	22	theme	guar	195:198	arg1	gum					200:202	hydroxypropyl guar gum	181:202	hydroxypropyl guar gum (HPG)	181:208	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	2	23	theme	PGSTE	384:388	arg1	measurements					390:401	PGSTE measurements	384:401	PGSTE measurements	384:401	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	0	24	dep	systems	77:83	arg1	rheological					12:22	rheological	12:22	rheological	12:22	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	0	24	dep	systems	77:83	arg1	Structural					0:9	Structural	0:9	Structural	0:9	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	0	24	dep	systems	77:83	arg1	dynamics					28:35	dynamics	28:35	dynamics	28:35	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	7	25	theme	set	1210:1212	arg1	up					1214:1215	the gel set up	1202:1215	the gel set up	1202:1215	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	2	26	with	formation	459:467	arg1	borax					494:498	borax	494:498	borax	494:498	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	2	27	theme	1H	377:378	arg1	NMR					380:382	1H NMR	377:382	1H NMR	377:382	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	5	28	theme	network	1053:1059	arg1	size					1041:1044	the mesh size	1032:1044	the mesh size of the network	1032:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	2	29	theme	glycerol	270:277	arg1	role					262:265	The role	258:265	The role	258:265	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	2	29	theme	glycerol	270:277	arg1	plasticizer					305:315	a plasticizer	303:315	a plasticizer in the formulation	303:334	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	3	30	theme	system	660:665	arg1	process					645:651	the relaxation process	630:651	the relaxation process of the system	630:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	4	31	theme	wide	839:842	arg1	range					844:848	a wide range	837:848	a wide range of compositions	837:864	The results obtained suggest that the mechanical properties of these gels can be tuned and controlled by modulating the formulation in a wide range of compositions.					
29519545	3	32	theme	rheological	568:578	arg1	behaviour					580:588	the rheological behaviour	564:588	the rheological behaviour	564:588	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	6	33	theme	systems	1117:1123	arg1	characteristics					1092:1106	The structural and mechanical characteristics	1062:1106	The structural and mechanical characteristics of these systems	1062:1123	The structural and mechanical characteristics of these systems suggest their potential use for applicative purposes.					
29519545	2	34	theme	11B	365:367	arg1	NMR					369:371	11B NMR	365:371	11B NMR	365:371	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	7	35	theme	La	1320:1321	arg1	Italy					1347:1351	Italy	1347:1351	Italy	1347:1351	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	35	theme	La	1320:1321	arg1	theatre					1330:1336	the La Fenice theatre	1316:1336	the La Fenice theatre (Venice, Italy)	1316:1352	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	3	36	theme	components	550:559	arg1	effect					535:540	The effect	531:540	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system	531:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	7	37	theme	cleaning	1249:1256	arg1	agent					1258:1262	cleaning agent	1249:1262	cleaning agent	1249:1262	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	37	theme	cleaning	1249:1256	arg1	up					1214:1215	the gel set up	1202:1215	the gel set up	1202:1215	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	37	theme	cleaning	1249:1256	arg1	one					1195:1197	one	1195:1197	one	1195:1197	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	4	38	theme	compositions	853:864	arg1	range					844:848	a wide range	837:848	a wide range of compositions	837:864	The results obtained suggest that the mechanical properties of these gels can be tuned and controlled by modulating the formulation in a wide range of compositions.					
29519545	0	39	theme	hydroxypropyl	49:61	arg1	Structural					0:9	Structural	0:9	Structural	0:9	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	7	40	from	fire	1447:1450	arg1	1996					1455:1458	1996	1455:1458	1996	1455:1458	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	1	41	contain	containing	170:179	arg2	borax					211:215	borax	211:215	borax	211:215	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	41	contain	containing	170:179	arg2	HPG					205:207	HPG	205:207	HPG	205:207	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	41	contain	containing	170:179	arg2	glycerol					221:228	glycerol	221:228	glycerol	221:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	41	contain	containing	170:179	arg2	gum					200:202	hydroxypropyl guar gum	181:202	hydroxypropyl guar gum (HPG)	181:208	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	1	41	contain	containing	170:179	arg1	systems					162:168	aqueous gel-like systems	145:168	aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	145:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	4	42	theme	mechanical	740:749	arg1	properties					751:760	the mechanical properties	736:760	the mechanical properties of these gels	736:774	The results obtained suggest that the mechanical properties of these gels can be tuned and controlled by modulating the formulation in a wide range of compositions.					
29519545	5	43	from	role	998:1001	arg1	size					1041:1044	the mesh size	1032:1044	the mesh size of the network	1032:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	5	44	theme	various	1010:1016	arg1	components					1018:1027	the various components	1006:1027	the various components	1006:1027	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	7	45	theme	Fenice	1323:1328	arg1	Italy					1347:1351	Italy	1347:1351	Italy	1347:1351	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	45	theme	Fenice	1323:1328	arg1	theatre					1330:1336	the La Fenice theatre	1316:1336	the La Fenice theatre (Venice, Italy)	1316:1352	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	5	46	theme	mesh	1036:1039	arg1	size					1041:1044	the mesh size	1032:1044	the mesh size of the network	1032:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	2	47	dep	NMR	369:371	arg1	measurements					390:401	PGSTE measurements	384:401	PGSTE measurements	384:401	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	2	47	dep	NMR	369:371	arg1	means					356:360	means	356:360	means	356:360	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	2	48	with	interaction	477:487	arg1	borax					494:498	borax	494:498	borax	494:498	The role of glycerol, which is introduced as a plasticizer in the formulation, is investigated by means of 11B NMR and 1H NMR PGSTE measurements in order to clarify its contribution to the gel network formation and its interaction with borax, with whom it forms a complex.					
29519545	7	49	theme	up	1214:1215	arg1	agent					1258:1262	cleaning agent	1249:1262	cleaning agent	1249:1262	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	49	theme	up	1214:1215	arg1	up					1214:1215	the gel set up	1202:1215	the gel set up	1202:1215	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	7	49	theme	up	1214:1215	arg1	one					1195:1197	one	1195:1197	one	1195:1197	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	5	50	theme	Small	945:949	arg1	SAXS					975:978	SAXS	975:978	SAXS	975:978	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	5	50	theme	Small	945:949	arg1	Scattering					963:972	Small Angle X-ray Scattering	945:972	Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network	945:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	6	51	theme	mechanical	1081:1090	arg1	characteristics					1092:1106	The structural and mechanical characteristics	1062:1106	The structural and mechanical characteristics of these systems	1062:1123	The structural and mechanical characteristics of these systems suggest their potential use for applicative purposes.					
29519545	1	52	theme	structural	114:123	arg1	characterization					125:140	A dynamic, rheological, and structural characterization	86:140	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	86:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29519545	5	53	theme	Angle	951:955	arg1	SAXS					975:978	SAXS	975:978	SAXS	975:978	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	5	53	theme	Angle	951:955	arg1	Scattering					963:972	Small Angle X-ray Scattering	945:972	Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network	945:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	3	54	theme	gels	545:548	arg1	components					550:559	gels components	545:559	gels components	545:559	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	7	55	theme	particulated	1407:1418	arg1	matter					1420:1425	particulated matter	1407:1425	particulated matter	1407:1425	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	0	56	theme	gel-like	68:75	arg1	systems					77:83	guar gel-like systems	63:83	guar gel-like systems	63:83	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	5	57	theme	X-ray	957:961	arg1	SAXS					975:978	SAXS	975:978	SAXS	975:978	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	5	57	theme	X-ray	957:961	arg1	Scattering					963:972	Small Angle X-ray Scattering	945:972	Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network	945:1059	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	6	58	theme	structural	1066:1075	arg1	characteristics					1092:1106	The structural and mechanical characteristics	1062:1106	The structural and mechanical characteristics of these systems	1062:1123	The structural and mechanical characteristics of these systems suggest their potential use for applicative purposes.					
29519545	7	59	theme	gel	1206:1208	arg1	up					1214:1215	the gel set up	1202:1215	the gel set up	1202:1215	In this regard, one of the gel set up has been successfully tested as cleaning agent on the surface of a XIX stucco fragment coming from the La Fenice theatre (Venice, Italy) for the removal of a dirt layer composed by dust and particulated matter originated during a fire in 1996.					
29519545	5	60	dep	Scattering	963:972	arg1	means					936:940	means	936:940	means	936:940	Moreover, a structural characterisation has been also carried out by means of Small Angle X-ray Scattering (SAXS) to highlight the role of the various components on the mesh size of the network.					
29519545	0	61	theme	guar	63:66	arg1	systems					77:83	guar gel-like systems	63:83	guar gel-like systems	63:83	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	3	62	theme	related	619:625	arg1	energy					612:617	the activation energy	597:617	the activation energy related to the relaxation process of the system	597:665	The effect of gels components on the rheological behaviour and on the activation energy related to the relaxation process of the system was assessed by means of rheology.					
29519545	0	63	dep	Structural	0:9	arg1	insights					37:44	insights	37:44	insights	37:44	Structural, rheological and dynamics insights of hydroxypropyl guar gel-like systems.					
29519545	1	64	theme	aqueous	145:151	arg1	systems					162:168	aqueous gel-like systems	145:168	aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol	145:228	A dynamic, rheological, and structural characterization of aqueous gel-like systems containing hydroxypropyl guar gum (HPG), borax and glycerol is presented in this paper.					
29966742	0	0	theme	hydrophobization	114:129	arg1	Effect					104:109	Effect	104:109	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	5	1	theme	substitution	852:863	arg1	concentration					898:910	α-cyclodextrin concentration	883:910	α-cyclodextrin concentration (0-10 wt%)	883:921	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	1	theme	substitution	852:863	arg1	%					920:920	0-10 wt%	913:920	0-10 wt%	913:920	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	1	theme	substitution	852:863	arg1	amylopectin					828:838	polysaccharide type (dextran, pullulan, amylopectin)	788:839	amylopectin	828:838	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	1	theme	substitution	852:863	arg1	degree					842:847	degree	842:847	degree of substitution (DS:0.1-5.6%)	842:877	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	2	theme	systematic	746:755	arg1	study					757:761	A systematic study	744:761	A systematic study	744:761	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	3	dep	amylopectin	828:838	arg1	pullulan					818:825	polysaccharide type (dextran, pullulan, amylopectin)	788:839	pullulan	818:825	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	4	theme	palmitoyl	983:991	arg1	groups					993:998	palmitoyl groups	983:998	palmitoyl groups (1 wt%)	983:1006	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	4	theme	palmitoyl	983:991	arg1	%					1005:1005	1 wt%	1001:1005	1 wt%	1001:1005	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	5	theme	dextran	809:815	arg1	amylopectin					828:838	polysaccharide type (dextran, pullulan, amylopectin)	788:839	amylopectin	828:838	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	6	6	theme	13C	1077:1079	arg1	NMR					1081:1083	solid state 13C NMR	1065:1083	solid state 13C NMR	1065:1083	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	0	7	theme	α-cyclodextrin	135:148	arg1	concentration					150:162	α-cyclodextrin concentration	135:162	α-cyclodextrin concentration	135:162	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	6	8	theme	state	1071:1075	arg1	NMR					1081:1083	solid state 13C NMR	1065:1083	solid state 13C NMR	1065:1083	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	0	9	from	α-cyclodextrin	88:101	arg1	platelets					28:36	Hierarchical supramolecular platelets	0:36	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	9	10	theme	polysaccharide	1570:1583	arg1	composition					1585:1595	polysaccharide composition	1570:1595	polysaccharide composition	1570:1595	This is the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition.					
29966742	6	11	theme	solid	1065:1069	arg1	NMR					1081:1083	solid state 13C NMR	1065:1083	solid state 13C NMR	1065:1083	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	4	12	theme	polysaccharide	716:729	arg1	composition					731:741	polysaccharide composition	716:741	polysaccharide composition	716:741	The objective of this investigation is to tune platelet formation by modifying these two parameters, independently on polysaccharide composition.					
29966742	8	13	theme	α-cyclodextrin	1357:1370	arg1	molecules					1372:1380	2 α-cyclodextrin molecules	1355:1380	2 α-cyclodextrin molecules	1355:1380	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	5	14	theme	fixed	929:933	arg1	concentration					935:947	a fixed concentration	927:947	a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%)	927:1006	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	0	15	theme	concentration	150:162	arg1	Effect					104:109	Effect	104:109	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	0	16	from	polysaccharides	68:82	arg1	platelets					28:36	Hierarchical supramolecular platelets	0:36	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	9	17	theme	platelet	1484:1491	arg1	formation					1493:1501	platelet formation	1484:1501	platelet formation	1484:1501	This is the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition.					
29966742	0	18	theme	supramolecular	13:26	arg1	platelets					28:36	Hierarchical supramolecular platelets	0:36	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	1	19	theme	particles	228:236	arg1	particles					228:236	particles	228:236	particles	228:236	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	1	19	theme	particles	228:236	arg1	nano-platelets					198:211	nano-platelets	198:211	nano-platelets	198:211	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	1	19	theme	particles	228:236	arg1	group					219:223	a group	217:223	a group of particles with typical flat surfaces and hexagonal shape	217:283	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	1	19	theme	particles	228:236	arg1	Micro-					187:192	Micro-	187:192	Micro-	187:192	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	0	20	theme	Hierarchical	0:11	arg1	platelets					28:36	Hierarchical supramolecular platelets	0:36	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	7	21	theme	α-cyclodextrin	1232:1245	arg1	DS					1195:1196	high DS	1190:1196	high DS (4.2-5.6%)	1190:1207	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	7	21	theme	α-cyclodextrin	1232:1245	arg1	%					1206:1206	4.2-5.6%	1199:1206	4.2-5.6%	1199:1206	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	7	21	theme	α-cyclodextrin	1232:1245	arg1	concentration					1215:1227	a concentration	1213:1227	a concentration of α-cyclodextrin higher than 2.5 wt%	1213:1265	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	7	22	theme	high	1190:1193	arg1	DS					1195:1196	high DS	1190:1196	high DS (4.2-5.6%)	1190:1207	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	7	22	theme	high	1190:1193	arg1	%					1206:1206	4.2-5.6%	1199:1206	4.2-5.6%	1199:1206	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	2	23	theme	polysaccharides	365:379	arg1	water					337:341	water	337:341	water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains	337:422	They are obtained by hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains.					
29966742	5	24	theme	polysaccharide	952:965	arg1	concentration					935:947	a fixed concentration	927:947	a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%)	927:1006	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	7	25	theme	higher	1247:1252	arg1	concentration					1215:1227	a concentration	1213:1227	a concentration of α-cyclodextrin higher than 2.5 wt%	1213:1265	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	1	26	theme	typical	243:249	arg1	surfaces					256:263	typical flat surfaces	243:263	typical flat surfaces	243:263	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	8	27	theme	titration	1279:1287	arg1	calorimetry					1289:1299	Isothermal titration calorimetry	1268:1299	Isothermal titration calorimetry	1268:1299	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	6	28	theme	elemental	1045:1053	arg1	analysis					1055:1062	ATR-FTIR, elemental analysis	1035:1062	analysis	1055:1062	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	0	29	theme	platelet	167:174	arg1	formation					176:184	platelet formation	167:184	platelet formation	167:184	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	2	30	theme	α-cyclodextrin	346:359	arg1	water					337:341	water	337:341	water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains	337:422	They are obtained by hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains.					
29966742	9	31	theme	α-cyclodextrin	1523:1536	arg1	concentration					1538:1550	α-cyclodextrin concentration	1523:1550	α-cyclodextrin concentration	1523:1550	This is the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition.					
29966742	1	32	theme	flat	251:254	arg1	surfaces					256:263	typical flat surfaces	243:263	typical flat surfaces	243:263	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	3	33	theme	cohesive	482:489	arg1	platelets					491:499	well structured and cohesive platelets	462:499	well structured and cohesive platelets	462:499	It is expected that the formation of well structured and cohesive platelets is driven by the interaction between alkyl chains grafted on polysaccharides and α-cyclodextrin.					
29966742	4	34	theme	platelet	645:652	arg1	formation					654:662	platelet formation	645:662	platelet formation	645:662	The objective of this investigation is to tune platelet formation by modifying these two parameters, independently on polysaccharide composition.					
29966742	7	35	theme	hexagonal	1151:1159	arg1	platelets					1161:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	8	36	theme	Isothermal	1268:1277	arg1	calorimetry					1289:1299	Isothermal titration calorimetry	1268:1299	Isothermal titration calorimetry	1268:1299	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	9	37	theme	first	1436:1440	arg1	report					1442:1447	the first report	1432:1447	the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition	1432:1595	This is the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition.					
29966742	9	37	theme	first	1436:1440	arg1	This					1424:1427	This	1424:1427	This	1424:1427	This is the first report showing the possibility to control platelet formation by modifying DS and α-cyclodextrin concentration, independently on polysaccharide composition.					
29966742	0	38	theme	hydrophobically-modified	43:66	arg1	polysaccharides					68:82	hydrophobically-modified polysaccharides	43:82	hydrophobically-modified polysaccharides	43:82	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	6	39	theme	ATR-FTIR	1035:1042	arg1	analysis					1055:1062	ATR-FTIR, elemental analysis	1035:1062	analysis	1055:1062	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	3	40	theme	platelets	491:499	arg1	formation					449:457	the formation	445:457	the formation of well structured and cohesive platelets	445:499	It is expected that the formation of well structured and cohesive platelets is driven by the interaction between alkyl chains grafted on polysaccharides and α-cyclodextrin.					
29966742	0	41	from	Effect	104:109	arg1	formation					176:184	platelet formation	167:184	platelet formation	167:184	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	7	42	theme	Abundant	1123:1130	arg1	platelets					1161:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	8	43	theme	molecules	1372:1380	arg1	stoichiometry					1338:1350	a stoichiometry	1336:1350	a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran	1336:1421	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	3	44	theme	structured	467:476	arg1	platelets					491:499	well structured and cohesive platelets	462:499	well structured and cohesive platelets	462:499	It is expected that the formation of well structured and cohesive platelets is driven by the interaction between alkyl chains grafted on polysaccharides and α-cyclodextrin.					
29966742	5	45	theme	polysaccharide	788:801	arg1	amylopectin					828:838	polysaccharide type (dextran, pullulan, amylopectin)	788:839	amylopectin	828:838	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	8	46	theme	palmitoyl	1388:1396	arg1	group					1398:1402	1 palmitoyl group	1386:1402	1 palmitoyl group grafted on dextran	1386:1421	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	8	47	theme	sequential	1312:1321	arg1	binding					1323:1329	a sequential binding	1310:1329	a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran	1310:1421	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	7	48	theme	well-organized	1136:1149	arg1	platelets					1161:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets	1123:1169	Abundant and well-organized hexagonal platelets were obtained with high DS (4.2-5.6%) and a concentration of α-cyclodextrin higher than 2.5 wt%.					
29966742	4	49	theme	investigation	620:632	arg1	objective					602:610	The objective	598:610	The objective of this investigation	598:632	The objective of this investigation is to tune platelet formation by modifying these two parameters, independently on polysaccharide composition.					
29966742	1	50	with	group	219:223	arg1	surfaces					256:263	typical flat surfaces	243:263	typical flat surfaces	243:263	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	1	50	with	group	219:223	arg1	shape					279:283	hexagonal shape	269:283	hexagonal shape	269:283	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	2	51	theme	hierarchical	307:318	arg1	self-assembly					320:332	hierarchical self-assembly	307:332	hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains	307:422	They are obtained by hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains.					
29966742	2	52	theme	alkyl	411:415	arg1	chains					417:422	alkyl chains	411:422	alkyl chains	411:422	They are obtained by hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains.					
29966742	2	53	from	self-assembly	320:332	arg1	water					337:341	water	337:341	water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains	337:422	They are obtained by hierarchical self-assembly in water of α-cyclodextrin and polysaccharides hydrophobically-modified with alkyl chains.					
29966742	1	54	theme	hexagonal	269:277	arg1	shape					279:283	hexagonal shape	269:283	hexagonal shape	269:283	Micro- and nano-platelets are a group of particles with typical flat surfaces and hexagonal shape.					
29966742	5	55	theme	type	803:806	arg1	amylopectin					828:838	polysaccharide type (dextran, pullulan, amylopectin)	788:839	amylopectin	828:838	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	0	56	dep	platelets	28:36	arg1	Effect					104:109	Effect	104:109	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.	0:185	Hierarchical supramolecular platelets from hydrophobically-modified polysaccharides and α-cyclodextrin: Effect of hydrophobization and α-cyclodextrin concentration on platelet formation.					
29966742	8	57	with	binding	1323:1329	arg1	stoichiometry					1338:1350	a stoichiometry	1336:1350	a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran	1336:1421	Isothermal titration calorimetry revealed a sequential binding with a stoichiometry of 2 α-cyclodextrin molecules for 1 palmitoyl group grafted on dextran.					
29966742	6	58	theme	electron	1102:1109	arg1	microscopy					1111:1120	transmission electron microscopy	1089:1120	transmission electron microscopy	1089:1120	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	3	59	theme	alkyl	538:542	arg1	chains					544:549	alkyl chains	538:549	alkyl chains grafted on polysaccharides and α-cyclodextrin	538:595	It is expected that the formation of well structured and cohesive platelets is driven by the interaction between alkyl chains grafted on polysaccharides and α-cyclodextrin.					
29966742	6	60	theme	transmission	1089:1100	arg1	microscopy					1111:1120	transmission electron microscopy	1089:1120	transmission electron microscopy	1089:1120	Characterizations include ATR-FTIR, elemental analysis, solid state 13C NMR and transmission electron microscopy.					
29966742	5	61	theme	α-cyclodextrin	883:896	arg1	concentration					898:910	α-cyclodextrin concentration	883:910	α-cyclodextrin concentration (0-10 wt%)	883:921	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
29966742	5	61	theme	α-cyclodextrin	883:896	arg1	%					920:920	0-10 wt%	913:920	0-10 wt%	913:920	A systematic study was conducted by varying polysaccharide type (dextran, pullulan, amylopectin), degree of substitution (DS:0.1-5.6%) and α-cyclodextrin concentration (0-10 wt%) for a fixed concentration of polysaccharide esterified with palmitoyl groups (1 wt%).					
30064746	4	0	dep	ratio	523:527	arg1	to					514:515	to	514:515	to	514:515	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	1	1	contain	possesses	146:154	arg1	polysaccharide					126:139	a marine sulfated polysaccharide	108:139	a marine sulfated polysaccharide that possesses various biological activities	108:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	1	1	contain	possesses	146:154	arg1	Fucoidan					96:103	Fucoidan	96:103	Fucoidan	96:103	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	1	1	contain	possesses	146:154	arg2	activities					175:184	various biological activities	156:184	various biological activities	156:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	9	2	theme	molecular	1160:1168	arg1	weight					1170:1175	the molecular weight	1156:1175	the molecular weight	1156:1175	Biological activities were improved as the molecular weight was reduced.					
30064746	8	3	theme	molecular	1090:1098	arg1	weight					1100:1105	low molecular weight	1086:1105	low molecular weight fucoidan	1086:1114	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	3	4	theme	low	334:336	arg1	weight					348:353	a low molecular weight	332:353	a low molecular weight fucoidan	332:362	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	4	5	theme	liquid	507:512	arg1	ratio					523:527	liquid to solid ratio	507:527	liquid to solid ratio	507:527	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	5	6	theme	chemical	630:637	arg1	composition					639:649	chemical composition	630:649	chemical composition	630:649	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	7	7	theme	anticoagulant	932:944	arg1	activity					946:953	anticoagulant activity	932:953	anticoagulant activity	932:953	Moreover, the optimized SCW-treated fucoidan was checked for cytotoxicity, antimicrobial, antidiabetic, and anticoagulant activity compared with the untreated fucoidan.					
30064746	8	8	theme	SCW	1028:1030	arg1	treatment					1032:1040	SCW treatment	1028:1040	SCW treatment	1028:1040	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	7	9	theme	optimized	838:846	arg1	fucoidan					860:867	the optimized SCW-treated fucoidan	834:867	the optimized SCW-treated fucoidan	834:867	Moreover, the optimized SCW-treated fucoidan was checked for cytotoxicity, antimicrobial, antidiabetic, and anticoagulant activity compared with the untreated fucoidan.					
30064746	4	10	theme	influences	460:469	arg1	influence					437:445	the influence	433:445	the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan	433:573	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	6	11	theme	SCW-treated	695:705	arg1	fucoidan					707:714	resulting SCW-treated fucoidan	685:714	resulting SCW-treated fucoidan	685:714	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	4	12	used	used	419:422	arg2	RSM					410:412	RSM	410:412	RSM	410:412	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	12	used	used	419:422	arg2	methodology					397:407	response surface methodology	380:407	response surface methodology (RSM)	380:413	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	6	13	theme	resulting	685:693	arg1	fucoidan					707:714	resulting SCW-treated fucoidan	685:714	resulting SCW-treated fucoidan	685:714	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	5	14	theme	antioxidant	605:615	arg1	activity					617:624	antioxidant activity	605:624	antioxidant activity	605:624	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	8	15	theme	low	1086:1088	arg1	weight					1100:1105	low molecular weight	1086:1105	low molecular weight fucoidan	1086:1114	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	0	16	theme	Hydrothermal	0:11	arg1	degradation					13:23	Hydrothermal degradation	0:23	Hydrothermal degradation of seaweed polysaccharide	0:49	Hydrothermal degradation of seaweed polysaccharide: Characterization and biological activities.					
30064746	8	17	theme	polymer	1053:1059	arg1	chain					1061:1065	polymer chain	1053:1065	polymer chain	1053:1065	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	4	18	theme	surface	389:395	arg1	RSM					410:412	RSM	410:412	RSM	410:412	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	18	theme	surface	389:395	arg1	methodology					397:407	response surface methodology	380:407	response surface methodology (RSM)	380:413	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	8	19	theme	obtained	997:1004	arg1	values					1006:1011	The obtained values	993:1011	The obtained values	993:1011	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	0	20	theme	seaweed	28:34	arg1	polysaccharide					36:49	seaweed polysaccharide	28:49	seaweed polysaccharide	28:49	Hydrothermal degradation of seaweed polysaccharide: Characterization and biological activities.					
30064746	4	21	dep	instance	475:482	arg1	ratio					523:527	liquid to solid ratio	507:527	liquid to solid ratio	507:527	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	21	dep	instance	475:482	arg1	agitation					534:542	agitation	534:542	agitation speed to depolymerize fucoidan	534:573	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	21	dep	instance	475:482	arg1	temperature					484:494	temperature	484:494	temperature	484:494	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	21	dep	instance	475:482	arg1	pressure					497:504	pressure	497:504	pressure	497:504	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	5	22	dep	activity	617:624	arg1	the					601:603	the	601:603	the	601:603	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	4	23	theme	response	380:387	arg1	RSM					410:412	RSM	410:412	RSM	410:412	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	4	23	theme	response	380:387	arg1	methodology					397:407	response surface methodology	380:407	response surface methodology (RSM)	380:413	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	1	24	theme	marine	110:115	arg1	polysaccharide					126:139	a marine sulfated polysaccharide	108:139	a marine sulfated polysaccharide that possesses various biological activities	108:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	1	24	theme	marine	110:115	arg1	Fucoidan					96:103	Fucoidan	96:103	Fucoidan	96:103	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	1	25	theme	sulfated	117:124	arg1	polysaccharide					126:139	a marine sulfated polysaccharide	108:139	a marine sulfated polysaccharide that possesses various biological activities	108:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	1	25	theme	sulfated	117:124	arg1	Fucoidan					96:103	Fucoidan	96:103	Fucoidan	96:103	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	5	26	used	used	584:587	arg2	RSM					576:578	RSM	576:578	RSM	576:578	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	3	27	theme	subcritical	287:297	arg1	treatment					305:313	subcritical water treatment	287:313	subcritical water treatment (SCW)	287:319	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	3	27	theme	subcritical	287:297	arg1	SCW					316:318	SCW	316:318	SCW	316:318	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	2	28	theme	functional	202:211	arg1	properties					213:222	the functional properties	198:222	the functional properties of fucoidan	198:234	To enhance the functional properties of fucoidan, it was depolymerized using a green technique viz.					
30064746	0	29	theme	polysaccharide	36:49	arg1	degradation					13:23	Hydrothermal degradation	0:23	Hydrothermal degradation of seaweed polysaccharide	0:49	Hydrothermal degradation of seaweed polysaccharide: Characterization and biological activities.					
30064746	4	30	theme	solid	517:521	arg1	ratio					523:527	liquid to solid ratio	507:527	liquid to solid ratio	507:527	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	3	31	theme	water	299:303	arg1	treatment					305:313	subcritical water treatment	287:313	subcritical water treatment (SCW)	287:319	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	3	31	theme	water	299:303	arg1	SCW					316:318	SCW	316:318	SCW	316:318	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	4	32	theme	different	450:458	arg1	influences					460:469	different influences	450:469	different influences	450:469	In this study, response surface methodology (RSM) was used to study the influence of different influences for instance temperature, pressure, liquid to solid ratio, and agitation speed to depolymerize fucoidan.					
30064746	0	33	dep	Characterization	52:67	arg1	degradation					13:23	Hydrothermal degradation	0:23	Hydrothermal degradation of seaweed polysaccharide	0:49	Hydrothermal degradation of seaweed polysaccharide: Characterization and biological activities.					
30064746	5	34	theme	SCW-treated	654:664	arg1	fucoidan					666:673	SCW-treated fucoidan	654:673	SCW-treated fucoidan	654:673	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	0	35	theme	biological	73:82	arg1	activities					84:93	biological activities	73:93	biological activities	73:93	Hydrothermal degradation of seaweed polysaccharide: Characterization and biological activities.					
30064746	9	36	theme	Biological	1117:1126	arg1	activities					1128:1137	Biological activities	1117:1137	Biological activities	1117:1137	Biological activities were improved as the molecular weight was reduced.					
30064746	5	37	theme	fucoidan	666:673	arg1	activity					617:624	antioxidant activity	605:624	antioxidant activity	605:624	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	5	37	theme	fucoidan	666:673	arg1	composition					639:649	chemical composition	630:649	chemical composition	630:649	RSM was used to focus on the antioxidant activity and chemical composition of SCW-treated fucoidan.					
30064746	6	38	theme	gravimetric	759:769	arg1	TGA					781:783	TGA	781:783	TGA	781:783	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	6	38	theme	gravimetric	759:769	arg1	analysis					771:778	UV-Vis, FT-IR, Thermal gravimetric analysis	736:778	UV-Vis, FT-IR, Thermal gravimetric analysis (TGA)	736:784	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	8	39	theme	weight	1100:1105	arg1	fucoidan					1107:1114	low molecular weight fucoidan	1086:1114	low molecular weight fucoidan	1086:1114	The obtained values displayed that SCW treatment breakdowns polymer chain and so it produces low molecular weight fucoidan.					
30064746	2	40	theme	fucoidan	227:234	arg1	properties					213:222	the functional properties	198:222	the functional properties of fucoidan	198:234	To enhance the functional properties of fucoidan, it was depolymerized using a green technique viz.					
30064746	6	41	theme	UV-Vis	736:741	arg1	TGA					781:783	TGA	781:783	TGA	781:783	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	6	41	theme	UV-Vis	736:741	arg1	analysis					771:778	UV-Vis, FT-IR, Thermal gravimetric analysis	736:778	UV-Vis, FT-IR, Thermal gravimetric analysis (TGA)	736:784	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	1	42	theme	various	156:162	arg1	activities					175:184	various biological activities	156:184	various biological activities	156:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	6	43	dep	UV-Vis	736:741	arg1	FT-IR					744:748	FT-IR	744:748	FT-IR	744:748	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	6	43	dep	UV-Vis	736:741	arg1	Thermal					751:757	Thermal	751:757	Thermal	751:757	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	3	44	theme	molecular	338:346	arg1	weight					348:353	a low molecular weight	332:353	a low molecular weight fucoidan	332:362	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	6	45	theme	Elemental	792:800	arg1	analysis					802:809	Elemental analysis	792:809	Elemental analysis	792:809	Further, resulting SCW-treated fucoidan was investigated by UV-Vis, FT-IR, Thermal gravimetric analysis (TGA), DSC, Elemental analysis, and ESI-MS.					
30064746	2	46	theme	technique	272:280	arg1	viz					282:284	a green technique viz	264:284	a green technique viz	264:284	To enhance the functional properties of fucoidan, it was depolymerized using a green technique viz.					
30064746	7	47	theme	untreated	973:981	arg1	fucoidan					983:990	the untreated fucoidan	969:990	the untreated fucoidan	969:990	Moreover, the optimized SCW-treated fucoidan was checked for cytotoxicity, antimicrobial, antidiabetic, and anticoagulant activity compared with the untreated fucoidan.					
30064746	1	48	theme	biological	164:173	arg1	activities					175:184	various biological activities	156:184	various biological activities	156:184	Fucoidan is a marine sulfated polysaccharide that possesses various biological activities.					
30064746	3	49	theme	weight	348:353	arg1	fucoidan					355:362	a low molecular weight fucoidan	332:362	a low molecular weight fucoidan	332:362	subcritical water treatment (SCW) to produce a low molecular weight fucoidan.					
30064746	2	50	theme	green	266:270	arg1	viz					282:284	a green technique viz	264:284	a green technique viz	264:284	To enhance the functional properties of fucoidan, it was depolymerized using a green technique viz.					
30064746	7	51	theme	SCW-treated	848:858	arg1	fucoidan					860:867	the optimized SCW-treated fucoidan	834:867	the optimized SCW-treated fucoidan	834:867	Moreover, the optimized SCW-treated fucoidan was checked for cytotoxicity, antimicrobial, antidiabetic, and anticoagulant activity compared with the untreated fucoidan.					
31756475	9	0	theme	HepG2	919:923	arg1	cells					937:941	HepG2 and SGC7901 cells	919:941	cells	937:941	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	6	1	theme	glycosyl	550:557	arg1	groups					559:564	glycosyl groups	550:564	glycosyl groups at the C-3 positions	550:585	The side chains bound to the main chain with glycosyl groups at the C-3 positions.					
31756475	0	2	theme	polysaccharide	87:100	arg1	activity					45:52	potential anti-tumor activity	24:52	potential anti-tumor activity	24:52	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	0	2	theme	polysaccharide	87:100	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	5	3	theme	main	414:417	arg1	chain					419:423	The main chain	410:423	The main chain of WM-NP-60	410:435	The main chain of WM-NP-60 was composed of β-1,6-D-Glcp and its side chains were β-1,3-D-Glcp.					
31756475	7	4	theme	side	631:634	arg1	Gal					588:590	Gal	588:590	Gal	588:590	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	7	4	theme	side	631:634	arg1	chain					636:640	a side chain	629:640	a side chain	629:640	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	6	5	theme	main	534:537	arg1	chain					539:543	the main chain	530:543	the main chain	530:543	The side chains bound to the main chain with glycosyl groups at the C-3 positions.					
31756475	10	6	theme	natural	1074:1080	arg1	WM-NP-60					989:996	WM-NP-60	989:996	WM-NP-60	989:996	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	6	theme	natural	1074:1080	arg1	drug					1093:1096	a potential natural anti-tumor drug	1062:1096	a potential natural anti-tumor drug	1062:1096	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	2	7	theme	WM-NP-60	295:302	arg1	characteristics					241:255	The characteristics	237:255	The characteristics	237:255	The characteristics and potential antitumor activities of WM-NP-60 were studied.					
31756475	2	7	theme	WM-NP-60	295:302	arg1	activities					281:290	potential antitumor activities	261:290	potential antitumor activities	261:290	The characteristics and potential antitumor activities of WM-NP-60 were studied.					
31756475	8	8	theme	cells	752:756	arg1	proliferation					717:729	the proliferation	713:729	the proliferation of HepG2 and SGC7901 cells	713:756	WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner.					
31756475	9	9	theme	cells	937:941	arg1	apoptosis					906:914	apoptosis	906:914	apoptosis of HepG2 and SGC7901 cells	906:941	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	10	10	theme	potential	1064:1072	arg1	WM-NP-60					989:996	WM-NP-60	989:996	WM-NP-60	989:996	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	10	theme	potential	1064:1072	arg1	drug					1093:1096	a potential natural anti-tumor drug	1062:1096	a potential natural anti-tumor drug	1062:1096	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	11	theme	anti-tumor	1082:1091	arg1	WM-NP-60					989:996	WM-NP-60	989:996	WM-NP-60	989:996	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	11	theme	anti-tumor	1082:1091	arg1	drug					1093:1096	a potential natural anti-tumor drug	1062:1096	a potential natural anti-tumor drug	1062:1096	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	0	12	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	10	13	theme	tumor-suppressive	1010:1026	arg1	activity					1028:1035	a tumor-suppressive activity	1008:1035	a tumor-suppressive activity	1008:1035	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	9	14	located	found	808:812	arg2	it					801:802	it	801:802	it	801:802	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	9	14	located	found	808:812	arg1	addition					791:798	addition	791:798	addition	791:798	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	0	15	theme	potential	24:32	arg1	activity					45:52	potential anti-tumor activity	24:52	potential anti-tumor activity	24:52	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	9	16	theme	SGC7901	852:858	arg1	arrest					871:876	the HepG2 and SGC7901 cell cycle arrest	838:876	arrest	871:876	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	1	17	theme	neutral	105:111	arg1	WM-NP-60					128:135	A neutral polysaccharide WM-NP-60	103:135	A neutral polysaccharide WM-NP-60	103:135	A neutral polysaccharide WM-NP-60 was successfully isolated and purified from a phytopathogenic fungus Sporisorium reilianum (Fries).					
31756475	9	18	theme	cell	860:863	arg1	arrest					871:876	the HepG2 and SGC7901 cell cycle arrest	838:876	arrest	871:876	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	0	19	theme	anti-tumor	34:43	arg1	activity					45:52	potential anti-tumor activity	24:52	potential anti-tumor activity	24:52	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	9	20	theme	cycle	865:869	arg1	arrest					871:876	the HepG2 and SGC7901 cell cycle arrest	838:876	arrest	871:876	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	9	21	theme	SGC7901	929:935	arg1	cells					937:941	HepG2 and SGC7901 cells	919:941	cells	937:941	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	8	22	theme	HepG2	734:738	arg1	proliferation					717:729	the proliferation	713:729	the proliferation of HepG2 and SGC7901 cells	713:756	WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner.					
31756475	6	23	theme	side	509:512	arg1	chains					514:519	The side chains	505:519	The side chains	505:519	The side chains bound to the main chain with glycosyl groups at the C-3 positions.					
31756475	1	24	theme	polysaccharide	113:126	arg1	WM-NP-60					128:135	A neutral polysaccharide WM-NP-60	103:135	A neutral polysaccharide WM-NP-60	103:135	A neutral polysaccharide WM-NP-60 was successfully isolated and purified from a phytopathogenic fungus Sporisorium reilianum (Fries).					
31756475	7	25	attach	attached	601:608	arg1	backbone					617:624	the backbone	613:624	the backbone	613:624	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	7	25	attach	attached	601:608	arg2	chain					636:640	a side chain	629:640	a side chain	629:640	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	7	25	attach	attached	601:608	arg2	Gal					588:590	Gal	588:590	Gal	588:590	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	3	26	theme	water-soluble	333:345	arg1	polysaccharide					347:360	a water-soluble polysaccharide	331:360	a water-soluble polysaccharide	331:360	WM-NP-60 was a water-soluble polysaccharide.					
31756475	3	26	theme	water-soluble	333:345	arg1	WM-NP-60					318:325	WM-NP-60	318:325	WM-NP-60	318:325	WM-NP-60 was a water-soluble polysaccharide.					
31756475	7	27	theme	β-1,3-D-Glcp	665:676	arg1	chain					683:687	the linear β-1,3-D-Glcp side chain	654:687	the linear β-1,3-D-Glcp side chain	654:687	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	7	28	theme	side	678:681	arg1	chain					683:687	the linear β-1,3-D-Glcp side chain	654:687	the linear β-1,3-D-Glcp side chain	654:687	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	8	29	theme	SGC7901	744:750	arg1	cells					752:756	SGC7901 cells	744:756	SGC7901 cells	744:756	WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner.					
31756475	6	30	from	positions	577:585	arg1	groups					559:564	glycosyl groups	550:564	glycosyl groups at the C-3 positions	550:585	The side chains bound to the main chain with glycosyl groups at the C-3 positions.					
31756475	4	31	theme	WM-NP-60	387:394	arg1	15.6 kDa					400:407	15.6 kDa	400:407	15.6 kDa	400:407	The molecular weight of WM-NP-60 was 15.6 kDa.					
31756475	4	31	theme	WM-NP-60	387:394	arg1	weight					377:382	The molecular weight	363:382	The molecular weight of WM-NP-60	363:394	The molecular weight of WM-NP-60 was 15.6 kDa.					
31756475	5	32	theme	side	474:477	arg1	chains					479:484	its side chains	470:484	its side chains were β-1,3-D-Glcp	470:502	The main chain of WM-NP-60 was composed of β-1,6-D-Glcp and its side chains were β-1,3-D-Glcp.					
31756475	9	33	theme	G1	885:886	arg1	phase					888:892	the G1 phase	881:892	the G1 phase	881:892	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	9	34	from	phase	888:892	arg1	arrest					871:876	the HepG2 and SGC7901 cell cycle arrest	838:876	arrest	871:876	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	9	34	from	phase	888:892	arg1	HepG2					842:846	the HepG2 and SGC7901 cell cycle arrest	838:876	HepG2	842:846	In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells.					
31756475	10	35	contain	possessed	998:1006	arg2	activity					1028:1035	a tumor-suppressive activity	1008:1035	a tumor-suppressive activity	1008:1035	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	35	contain	possessed	998:1006	arg1	WM-NP-60					989:996	WM-NP-60	989:996	WM-NP-60	989:996	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	10	35	contain	possessed	998:1006	arg1	drug					1093:1096	a potential natural anti-tumor drug	1062:1096	a potential natural anti-tumor drug	1062:1096	Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.					
31756475	6	36	theme	C-3	573:575	arg1	positions					577:585	the C-3 positions	569:585	the C-3 positions	569:585	The side chains bound to the main chain with glycosyl groups at the C-3 positions.					
31756475	7	37	theme	linear	658:663	arg1	chain					683:687	the linear β-1,3-D-Glcp side chain	654:687	the linear β-1,3-D-Glcp side chain	654:687	Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain.					
31756475	2	38	theme	antitumor	271:279	arg1	activities					281:290	potential antitumor activities	261:290	potential antitumor activities	261:290	The characteristics and potential antitumor activities of WM-NP-60 were studied.					
31756475	5	39	theme	WM-NP-60	428:435	arg1	chain					419:423	The main chain	410:423	The main chain of WM-NP-60	410:435	The main chain of WM-NP-60 was composed of β-1,6-D-Glcp and its side chains were β-1,3-D-Glcp.					
31756475	0	40	theme	reilianum	69:77	arg1	polysaccharide					87:100	Sporisorium reilianum (Fries) polysaccharide	57:100	Sporisorium reilianum (Fries) polysaccharide	57:100	Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide.					
31756475	2	41	theme	potential	261:269	arg1	activities					281:290	potential antitumor activities	261:290	potential antitumor activities	261:290	The characteristics and potential antitumor activities of WM-NP-60 were studied.					
31756475	4	42	theme	molecular	367:375	arg1	15.6 kDa					400:407	15.6 kDa	400:407	15.6 kDa	400:407	The molecular weight of WM-NP-60 was 15.6 kDa.					
31756475	4	42	theme	molecular	367:375	arg1	weight					377:382	The molecular weight	363:382	The molecular weight of WM-NP-60	363:394	The molecular weight of WM-NP-60 was 15.6 kDa.					
31756475	8	43	theme	dose-dependently	763:778	arg1	manner					780:785	a dose-dependently manner	761:785	a dose-dependently manner	761:785	WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner.					
30794901	0	0	theme	green	102:106	arg1	tea					108:110	Se-enriched green tea	90:110	Se-enriched green tea	90:110	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	3	1	theme	NSe-TPS2	439:446	arg1	structures					412:421	The structures	408:421	The structures of ASe-TPS2 and NSe-TPS2	408:446	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	6	2	theme	α‑D‑	984:987	arg1	-GalpA					995:1000	α‑D‑(1 → 4)-GalpA	984:1000	α‑D‑(1 → 4)-GalpA	984:1000	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	0	3	theme	Se-enriched	90:100	arg1	tea					108:110	Se-enriched green tea	90:110	Se-enriched green tea	90:110	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	5	4	theme	α‑D‑	780:783	arg1	‑GalpA					791:796	α‑D‑(1 → 4)‑GalpA	780:796	α‑D‑(1 → 4)‑GalpA	780:796	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	2	5	theme	molecular	354:362	arg1	weights					364:370	the molecular weights	350:370	the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	350:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	4	6	contain	containing	670:679	arg2	acid					703:706	uronic acid	696:706	uronic acid	696:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	4	6	contain	containing	670:679	arg1	polysaccharides					654:668	acidic polysaccharides	647:668	acidic polysaccharides containing high amount of uronic acid	647:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	4	6	contain	containing	670:679	arg2	amount					686:691	high amount	681:691	high amount of uronic acid	681:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	7	7	theme	different	1261:1269	arg1	structures					1292:1301	different polysaccharide chain structures	1261:1301	different polysaccharide chain structures	1261:1301	These results suggested that the distinction of selenylation methods could present different polysaccharide chain structures.					
30794901	1	8	theme	functional	161:170	arg1	food					172:175	a functional food	159:175	a functional food	159:175	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	8	theme	functional	161:170	arg1	tea					131:133	Se-enriched green tea	113:133	Se-enriched green tea	113:133	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	3	9	theme	TGA	591:593	arg1	analysis					595:602	TGA analysis	591:602	TGA analysis	591:602	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	0	10	from	natural	67:73	arg1	Comparison					0:9	Comparison	0:9	Comparison	0:9	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	0	10	from	natural	67:73	arg1	characterization					26:41	structural characterization	15:41	structural characterization	15:41	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	6	11	theme	1 → 2	1088:1092	arg1	‑Rhap					1094:1098	β‑L‑(1 → 2)‑Rhap	1083:1098	β‑L‑(1 → 2)‑Rhap	1083:1098	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	6	12	theme	β‑L‑	1044:1047	arg1	‑Araf					1055:1059	β‑L‑(1 → 2)‑Araf	1044:1059	β‑L‑(1 → 2)‑Araf	1044:1059	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	3	13	theme	acid	515:518	arg1	hydrolysis					520:529	partial acid hydrolysis	507:529	partial acid hydrolysis	507:529	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	5	14	theme	β‑D‑	762:765	arg1	‑Glcp					773:777	β‑D‑(1 → 3)‑Glcp	762:777	β‑D‑(1 → 3)‑Glcp	762:777	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	4	15	theme	acidic	647:652	arg1	polysaccharides					654:668	acidic polysaccharides	647:668	acidic polysaccharides containing high amount of uronic acid	647:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	7	16	theme	methods	1239:1245	arg1	distinction					1211:1221	the distinction	1207:1221	the distinction of selenylation methods	1207:1245	These results suggested that the distinction of selenylation methods could present different polysaccharide chain structures.					
30794901	2	17	theme	2.44 × 105 Da	393:405	arg1	weights					364:370	the molecular weights	350:370	the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	350:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	5	18	theme	1 → 4	839:843	arg1	‑GalpA					845:850	α‑D‑(1 → 4)‑GalpA	834:850	α‑D‑(1 → 4)‑GalpA	834:850	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	3	19	theme	ASe-TPS2	426:433	arg1	structures					412:421	The structures	408:421	The structures of ASe-TPS2 and NSe-TPS2	408:446	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	1	20	used	used	151:154	arg2	tea					131:133	Se-enriched green tea	113:133	Se-enriched green tea	113:133	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	20	used	used	151:154	arg2	prevention					189:198	disease prevention	181:198	disease prevention	181:198	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	20	used	used	151:154	arg2	food					172:175	a functional food	159:175	a functional food	159:175	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	5	21	theme	1 → 2	818:822	arg1	‑Rhap					824:828	α‑L‑(1 → 2)‑Rhap	813:828	α‑L‑(1 → 2)‑Rhap	813:828	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	7	22	theme	chain	1286:1290	arg1	structures					1292:1301	different polysaccharide chain structures	1261:1301	different polysaccharide chain structures	1261:1301	These results suggested that the distinction of selenylation methods could present different polysaccharide chain structures.					
30794901	2	23	theme	natural	320:326	arg1	teas					340:343	artificial and natural Se-enriched teas	305:343	artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	305:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	1	24	theme	disease	181:187	arg1	tea					131:133	Se-enriched green tea	113:133	Se-enriched green tea	113:133	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	24	theme	disease	181:187	arg1	prevention					189:198	disease prevention	181:198	disease prevention	181:198	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	5	25	theme	non-reducing	861:872	arg1	ends					874:877	the non-reducing ends	857:877	the non-reducing ends	857:877	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	4	26	theme	uronic	696:701	arg1	acid					703:706	uronic acid	696:706	uronic acid	696:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	7	27	theme	selenylation	1226:1237	arg1	methods					1239:1245	selenylation methods	1226:1245	selenylation methods	1226:1245	These results suggested that the distinction of selenylation methods could present different polysaccharide chain structures.					
30794901	6	28	theme	α‑D‑	1062:1065	arg1	‑Galp					1073:1077	α‑D‑(1 → 3)‑Galp	1062:1077	α‑D‑(1 → 3)‑Galp	1062:1077	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	2	29	theme	artificial	305:314	arg1	teas					340:343	artificial and natural Se-enriched teas	305:343	artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	305:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	6	30	theme	β‑D-	963:966	arg1	‑Glcp					974:978	β‑D-(1 → 4)‑Glcp	963:978	β‑D-(1 → 4)‑Glcp	963:978	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	0	31	theme	structural	15:24	arg1	characterization					26:41	structural characterization	15:41	structural characterization	15:41	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	5	32	theme	ASe-TPS2	730:737	arg1	structure					713:721	The structure	709:721	The structure of the ASe-TPS2	709:737	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	2	33	with	teas	340:343	arg1	weights					364:370	the molecular weights	350:370	the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	350:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	1	34	theme	green	125:129	arg1	tea					131:133	Se-enriched green tea	113:133	Se-enriched green tea	113:133	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	34	theme	green	125:129	arg1	prevention					189:198	disease prevention	181:198	disease prevention	181:198	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	34	theme	green	125:129	arg1	food					172:175	a functional food	159:175	a functional food	159:175	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	3	35	theme	FT-IR	545:549	arg1	spectroscopy					551:562	FT-IR spectroscopy	545:562	FT-IR spectroscopy	545:562	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	0	36	theme	polysaccharides	46:60	arg1	Comparison					0:9	Comparison	0:9	Comparison	0:9	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	0	36	theme	polysaccharides	46:60	arg1	characterization					26:41	structural characterization	15:41	structural characterization	15:41	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	4	37	theme	acid	703:706	arg1	amount					686:691	high amount	681:691	high amount of uronic acid	681:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	4	37	theme	acid	703:706	arg1	acid					703:706	uronic acid	696:706	uronic acid	696:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	7	38	theme	polysaccharide	1271:1284	arg1	structures					1292:1301	different polysaccharide chain structures	1261:1301	different polysaccharide chain structures	1261:1301	These results suggested that the distinction of selenylation methods could present different polysaccharide chain structures.					
30794901	4	39	theme	high	681:684	arg1	amount					686:691	high amount	681:691	high amount of uronic acid	681:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	4	39	theme	high	681:684	arg1	acid					703:706	uronic acid	696:706	uronic acid	696:706	It showed that ASe-TPS2 and NSe-TPS2 were acidic polysaccharides containing high amount of uronic acid.					
30794901	0	40	dep	natural	67:73	arg1	tea					108:110	Se-enriched green tea	90:110	Se-enriched green tea	90:110	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	6	41	theme	1 → 2	1049:1053	arg1	‑Araf					1055:1059	β‑L‑(1 → 2)‑Araf	1044:1059	β‑L‑(1 → 2)‑Araf	1044:1059	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	3	42	theme	monosaccharide	470:483	arg1	analysis					497:504	monosaccharide composition analysis	470:504	monosaccharide composition analysis	470:504	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	6	43	theme	1 → 4	968:972	arg1	‑Glcp					974:978	β‑D-(1 → 4)‑Glcp	963:978	β‑D-(1 → 4)‑Glcp	963:978	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	0	44	from	artificial	79:88	arg1	Comparison					0:9	Comparison	0:9	Comparison	0:9	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	0	44	from	artificial	79:88	arg1	characterization					26:41	structural characterization	15:41	structural characterization	15:41	Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea.					
30794901	6	45	theme	1 → 3	1067:1071	arg1	‑Galp					1073:1077	α‑D‑(1 → 3)‑Galp	1062:1077	α‑D‑(1 → 3)‑Galp	1062:1077	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	5	46	theme	α‑L‑	813:816	arg1	‑Rhap					824:828	α‑L‑(1 → 2)‑Rhap	813:828	α‑L‑(1 → 2)‑Rhap	813:828	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	2	47	dep	kinds	220:224	arg1	kinds					220:224	two kinds	216:224	two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2	216:284	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	2	47	dep	kinds	220:224	arg1	NSe-TPS2					277:284	NSe-TPS2	277:284	NSe-TPS2	277:284	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	2	47	dep	kinds	220:224	arg1	ASe-TPS2					264:271	ASe-TPS2	264:271	ASe-TPS2	264:271	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	5	48	theme	1 → 4	785:789	arg1	‑GalpA					791:796	α‑D‑(1 → 4)‑GalpA	780:796	α‑D‑(1 → 4)‑GalpA	780:796	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	6	49	theme	1 → 4	989:993	arg1	-GalpA					995:1000	α‑D‑(1 → 4)-GalpA	984:1000	α‑D‑(1 → 4)-GalpA	984:1000	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	6	50	theme	β‑L‑	1083:1086	arg1	‑Rhap					1094:1098	β‑L‑(1 → 2)‑Rhap	1083:1098	β‑L‑(1 → 2)‑Rhap	1083:1098	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	5	51	dep	‑Glcp	806:810	arg1	1 → 4					800:804	1 → 4	800:804	1 → 4	800:804	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	6	52	theme	non-reducing	1112:1123	arg1	ends					1125:1128	the non-reducing ends	1108:1128	the non-reducing ends	1108:1128	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	3	53	theme	partial	507:513	arg1	hydrolysis					520:529	partial acid hydrolysis	507:529	partial acid hydrolysis	507:529	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	1	54	theme	Se-enriched	113:123	arg1	tea					131:133	Se-enriched green tea	113:133	Se-enriched green tea	113:133	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	54	theme	Se-enriched	113:123	arg1	prevention					189:198	disease prevention	181:198	disease prevention	181:198	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	1	54	theme	Se-enriched	113:123	arg1	food					172:175	a functional food	159:175	a functional food	159:175	Se-enriched green tea has been widely used as a functional food and disease prevention.					
30794901	3	55	theme	NMR	565:567	arg1	spectroscopy					569:580	NMR spectroscopy	565:580	NMR spectroscopy	565:580	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	2	56	theme	Se-enriched	328:338	arg1	teas					340:343	artificial and natural Se-enriched teas	305:343	artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	305:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	5	57	theme	1 → 3	767:771	arg1	‑Glcp					773:777	β‑D‑(1 → 3)‑Glcp	762:777	β‑D‑(1 → 3)‑Glcp	762:777	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
30794901	3	58	theme	composition	485:495	arg1	analysis					497:504	monosaccharide composition analysis	470:504	monosaccharide composition analysis	470:504	The structures of ASe-TPS2 and NSe-TPS2 were characterized by monosaccharide composition analysis, partial acid hydrolysis, methylation, FT-IR spectroscopy, NMR spectroscopy, SEM and TGA analysis.					
30794901	2	59	theme	polysaccharides	241:255	arg1	kinds					220:224	two kinds	216:224	two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2	216:284	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	2	59	theme	polysaccharides	241:255	arg1	NSe-TPS2					277:284	NSe-TPS2	277:284	NSe-TPS2	277:284	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	2	59	theme	polysaccharides	241:255	arg1	ASe-TPS2					264:271	ASe-TPS2	264:271	ASe-TPS2	264:271	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	2	60	theme	6.73 × 103 Da	375:387	arg1	weights					364:370	the molecular weights	350:370	the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da	350:405	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	6	61	dep	Glcp	1154:1157	arg1	residues					1168:1175	residues	1168:1175	residues	1168:1175	However, the NSe-TPS2 was mainly composed of β‑D-(1 → 4)‑Glcp and α‑D‑(1 → 4)-GalpA, and the branches were mainly composed of β‑L‑(1 → 2)‑Araf, α‑D‑(1 → 3)‑Galp and β‑L‑(1 → 2)‑Rhap whereas the non-reducing ends were mainly composed of Glcp and Galp residues.					
30794901	2	62	theme	homogeneous	229:239	arg1	polysaccharides					241:255	homogeneous polysaccharides	229:255	homogeneous polysaccharides	229:255	In this study, two kinds of homogeneous polysaccharides namely ASe-TPS2 and NSe-TPS2 were obtained from artificial and natural Se-enriched teas with the molecular weights of 6.73 × 103 Da and 2.44 × 105 Da.					
30794901	5	63	theme	α‑D‑	834:837	arg1	‑GalpA					845:850	α‑D‑(1 → 4)‑GalpA	834:850	α‑D‑(1 → 4)‑GalpA	834:850	The structure of the ASe-TPS2 was mainly composed of β‑D‑(1 → 3)‑Glcp, α‑D‑(1 → 4)‑GalpA, (1 → 4)‑Glcp, α‑L‑(1 → 2)‑Rhap and α‑D‑(1 → 4)‑GalpA, and the non-reducing ends were mainly composed of Araf and Xylp.					
31826501	7	0	theme	films	1190:1194	arg1	plasticizers					1158:1169	eco-friendly and cost-effective plasticizers	1126:1169	eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP)	1126:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	0	theme	films	1190:1194	arg1	polysaccharides					1097:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	4	1	theme	acid	804:807	arg1	presence					725:732	the presence	721:732	the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains	721:869	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	8	2	theme	polymer	1298:1304	arg1	temperature					1279:1289	glass transition temperature	1262:1289	glass transition temperature of the polymer	1262:1304	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	0	3	theme	alginate	88:95	arg1	polysaccharides					25:39	polysaccharides	25:39	polysaccharides	25:39	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	0	3	theme	alginate	88:95	arg1	plasticizers					65:76	natural eco-friendly plasticizers	44:76	natural eco-friendly plasticizers of sodium alginate	44:95	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	6	4	theme	molecular	1001:1009	arg1	weight					1011:1016	the high molecular weight	992:1016	the high molecular weight	992:1016	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	4	5	theme	galacturonic	791:802	arg1	acid					804:807	galacturonic acid	791:807	galacturonic acid	791:807	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	7	6	theme	sodium	1174:1179	arg1	ALP					1205:1207	ALP	1205:1207	ALP	1205:1207	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	6	theme	sodium	1174:1179	arg1	AFP					1197:1199	AFP	1197:1199	AFP	1197:1199	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	6	theme	sodium	1174:1179	arg1	films					1190:1194	sodium alginate films	1174:1194	sodium alginate films (AFP and ALP)	1174:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	3	7	theme	fennel	639:644	arg1	polysaccharides					646:660	lemon and fennel polysaccharides	629:660	lemon and fennel polysaccharides (LP and FP)	629:672	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	7	theme	fennel	639:644	arg1	FP					670:671	FP	670:671	FP	670:671	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	7	theme	fennel	639:644	arg1	LP					663:664	LP	663:664	LP	663:664	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	4	8	theme	typical	810:816	arg1	xyslose					737:743	xyslose	737:743	xyslose	737:743	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	4	9	theme	glucose	757:763	arg1	monomers					778:785	glucose and rhamnose monomers	757:785	glucose and rhamnose monomers	757:785	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	2	10	theme	films	292:296	arg1	plasticizers					250:261	natural plasticizers	242:261	natural plasticizers of sodium alginate (A) based films	242:296	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	2	10	theme	films	292:296	arg1	polysaccharides					208:222	These polysaccharides	202:222	These polysaccharides	202:222	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	7	11	theme	alginate	1181:1188	arg1	ALP					1205:1207	ALP	1205:1207	ALP	1205:1207	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	11	theme	alginate	1181:1188	arg1	AFP					1197:1199	AFP	1197:1199	AFP	1197:1199	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	11	theme	alginate	1181:1188	arg1	films					1190:1194	sodium alginate films	1174:1194	sodium alginate films (AFP and ALP)	1174:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	8	12	theme	plasticized	1466:1476	arg1	system					1478:1483	a plasticized system	1464:1483	a plasticized system	1464:1483	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	2	13	theme	based	286:290	arg1	films					292:296	sodium alginate (A) based films	266:296	sodium alginate (A) based films	266:296	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	8	14	theme	glass	1262:1266	arg1	transition					1268:1277	glass transition	1262:1277	glass transition temperature of the polymer	1262:1304	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	4	15	theme	rhamnose	769:776	arg1	monomers					778:785	glucose and rhamnose monomers	757:785	glucose and rhamnose monomers	757:785	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	6	16	theme	high	996:999	arg1	weight					1011:1016	the high molecular weight	992:1016	the high molecular weight	992:1016	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	8	17	theme	DSC	1211:1213	arg1	analysis					1215:1222	DSC analysis	1211:1222	DSC analysis	1211:1222	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	6	18	theme	polysaccharides	1031:1045	arg1	weight					1011:1016	the high molecular weight	992:1016	the high molecular weight	992:1016	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	6	19	theme	fraction	1073:1080	arg1	weight					1011:1016	the high molecular weight	992:1016	the high molecular weight	992:1016	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	3	20	theme	ultrasound	506:515	arg1	UAE					538:540	UAE	538:540	UAE	538:540	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	20	theme	ultrasound	506:515	arg1	extraction					526:535	ultrasound assisted extraction	506:535	ultrasound assisted extraction (UAE)	506:541	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	8	21	theme	AFP	1430:1432	arg1	films					1442:1446	AFP and ALP films	1430:1446	AFP and ALP films	1430:1446	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	1	22	theme	secondary-raw	164:176	arg1	sources					193:199	secondary-raw polysaccharide sources	164:199	secondary-raw polysaccharide sources	164:199	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	1	22	theme	secondary-raw	164:176	arg1	wastes					130:135	lemon and fennel wastes	113:135	lemon and fennel wastes	113:135	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	2	23	theme	eco-friendly	435:446	arg1	way					448:450	a cost effective and eco-friendly way	414:450	a cost effective and eco-friendly way	414:450	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	8	24	theme	TGA	1377:1379	arg1	analysis					1381:1388	TGA analysis	1377:1388	TGA analysis	1377:1388	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	8	25	theme	elongation	1353:1362	arg1	rising					1343:1348	an enlightened rising	1328:1348	an enlightened rising of elongation	1328:1362	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	8	26	theme	enlightened	1331:1341	arg1	rising					1343:1348	an enlightened rising	1328:1348	an enlightened rising of elongation	1328:1362	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	1	27	theme	polysaccharide	178:191	arg1	sources					193:199	secondary-raw polysaccharide sources	164:199	secondary-raw polysaccharide sources	164:199	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	1	27	theme	polysaccharide	178:191	arg1	wastes					130:135	lemon and fennel wastes	113:135	lemon and fennel wastes	113:135	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	0	28	theme	Vegetable	0:8	arg1	wastes					10:15	Vegetable wastes	0:15	Vegetable wastes	0:15	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	8	29	theme	tensile	1307:1313	arg1	tests					1315:1319	tensile tests	1307:1319	tensile tests	1307:1319	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	4	30	theme	monomers	778:785	arg1	presence					725:732	the presence	721:732	the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains	721:869	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	4	31	theme	TLC	698:700	arg1	analyses					702:709	HPAE-PAD and TLC analyses	685:709	HPAE-PAD and TLC analyses	685:709	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	8	32	theme	faster	1399:1404	arg1	kinetics					1418:1425	a faster degradation kinetics	1397:1425	a faster degradation kinetics of AFP and ALP films	1397:1446	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	2	33	theme	effective	421:429	arg1	way					448:450	a cost effective and eco-friendly way	414:450	a cost effective and eco-friendly way	414:450	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	8	34	theme	transition	1268:1277	arg1	temperature					1279:1289	glass transition temperature	1262:1289	glass transition temperature of the polymer	1262:1304	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	4	35	theme	xyloglucan	853:862	arg1	chains					864:869	xyloglucan chains	853:869	xyloglucan chains	853:869	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	4	36	theme	HPAE-PAD	685:692	arg1	analyses					702:709	HPAE-PAD and TLC analyses	685:709	HPAE-PAD and TLC analyses	685:709	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	3	37	theme	Different	453:461	arg1	extraction					566:575	microwave assisted extraction	547:575	microwave assisted extraction (MAE)	547:581	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	37	theme	Different	453:461	arg1	processes					469:477	Different green processes	453:477	Different green processes	453:477	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	37	theme	Different	453:461	arg1	extraction					526:535	ultrasound assisted extraction	506:535	ultrasound assisted extraction (UAE)	506:541	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	37	theme	Different	453:461	arg1	maceration					488:497	maceration	488:497	maceration (MAC)	488:503	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	7	38	theme	eco-friendly	1126:1137	arg1	plasticizers					1158:1169	eco-friendly and cost-effective plasticizers	1126:1169	eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP)	1126:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	38	theme	eco-friendly	1126:1137	arg1	polysaccharides					1097:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	3	39	dep	polysaccharides	646:660	arg1	polysaccharides					646:660	lemon and fennel polysaccharides	629:660	lemon and fennel polysaccharides (LP and FP)	629:672	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	39	dep	polysaccharides	646:660	arg1	FP					670:671	FP	670:671	FP	670:671	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	39	dep	polysaccharides	646:660	arg1	LP					663:664	LP	663:664	LP	663:664	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	8	40	theme	degradation	1406:1416	arg1	kinetics					1418:1425	a faster degradation kinetics	1397:1425	a faster degradation kinetics of AFP and ALP films	1397:1446	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	3	41	theme	green	463:467	arg1	extraction					566:575	microwave assisted extraction	547:575	microwave assisted extraction (MAE)	547:581	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	41	theme	green	463:467	arg1	processes					469:477	Different green processes	453:477	Different green processes	453:477	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	41	theme	green	463:467	arg1	extraction					526:535	ultrasound assisted extraction	506:535	ultrasound assisted extraction (UAE)	506:541	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	41	theme	green	463:467	arg1	maceration					488:497	maceration	488:497	maceration (MAC)	488:503	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	0	42	theme	natural	44:50	arg1	polysaccharides					25:39	polysaccharides	25:39	polysaccharides	25:39	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	0	42	theme	natural	44:50	arg1	plasticizers					65:76	natural eco-friendly plasticizers	44:76	natural eco-friendly plasticizers of sodium alginate	44:95	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	7	43	theme	cost-effective	1143:1156	arg1	plasticizers					1158:1169	eco-friendly and cost-effective plasticizers	1126:1169	eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP)	1126:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	43	theme	cost-effective	1143:1156	arg1	polysaccharides					1097:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	4	44	theme	galactose	746:754	arg1	presence					725:732	the presence	721:732	the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains	721:869	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	8	45	theme	temperature	1279:1289	arg1	significant					1236:1246	significant	1236:1246	significant	1236:1246	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	2	46	theme	natural	242:248	arg1	plasticizers					250:261	natural plasticizers	242:261	natural plasticizers of sodium alginate (A) based films	242:296	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	2	46	theme	natural	242:248	arg1	polysaccharides					208:222	These polysaccharides	202:222	These polysaccharides	202:222	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	2	47	theme	potential	389:397	arg1	application					399:409	its potential application	385:409	its potential application in a cost effective and eco-friendly way	385:450	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	7	48	theme	extracted	1087:1095	arg1	plasticizers					1158:1169	eco-friendly and cost-effective plasticizers	1126:1169	eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP)	1126:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	48	theme	extracted	1087:1095	arg1	polysaccharides					1097:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	4	49	theme	polysaccharides	821:835	arg1	typical					810:816	typical	810:816	typical	810:816	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	3	50	theme	microwave	547:555	arg1	extraction					566:575	microwave assisted extraction	547:575	microwave assisted extraction (MAE)	547:581	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	50	theme	microwave	547:555	arg1	MAE					578:580	MAE	578:580	MAE	578:580	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	1	51	theme	lemon	113:117	arg1	wastes					130:135	lemon and fennel wastes	113:135	lemon and fennel wastes	113:135	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	1	51	theme	lemon	113:117	arg1	sources					193:199	secondary-raw polysaccharide sources	164:199	secondary-raw polysaccharide sources	164:199	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	0	52	theme	eco-friendly	52:63	arg1	polysaccharides					25:39	polysaccharides	25:39	polysaccharides	25:39	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	0	52	theme	eco-friendly	52:63	arg1	plasticizers					65:76	natural eco-friendly plasticizers	44:76	natural eco-friendly plasticizers of sodium alginate	44:95	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	7	53	dep	films	1190:1194	arg1	ALP					1205:1207	ALP	1205:1207	ALP	1205:1207	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	53	dep	films	1190:1194	arg1	AFP					1197:1199	AFP	1197:1199	AFP	1197:1199	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	53	dep	films	1190:1194	arg1	films					1190:1194	sodium alginate films	1174:1194	sodium alginate films (AFP and ALP)	1174:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	3	54	theme	assisted	557:564	arg1	extraction					566:575	microwave assisted extraction	547:575	microwave assisted extraction (MAE)	547:581	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	54	theme	assisted	557:564	arg1	MAE					578:580	MAE	578:580	MAE	578:580	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	2	55	theme	alginate	326:333	arg1	performances					335:346	sodium alginate performances	319:346	sodium alginate performances	319:346	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	7	56	used	used	1118:1121	arg2	plasticizers					1158:1169	eco-friendly and cost-effective plasticizers	1126:1169	eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP)	1126:1208	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	7	56	used	used	1118:1121	arg2	polysaccharides					1097:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides	1083:1111	The extracted polysaccharides were used as eco-friendly and cost-effective plasticizers of sodium alginate films (AFP and ALP).					
31826501	3	57	theme	lemon	629:633	arg1	polysaccharides					646:660	lemon and fennel polysaccharides	629:660	lemon and fennel polysaccharides (LP and FP)	629:672	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	57	theme	lemon	629:633	arg1	FP					670:671	FP	670:671	FP	670:671	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	57	theme	lemon	629:633	arg1	LP					663:664	LP	663:664	LP	663:664	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	5	58	theme	spectroscopic	918:930	arg1	analyses					932:939	FTIR spectroscopic analyses	913:939	FTIR spectroscopic analyses	913:939	These findings were confirmed by NMR and FTIR spectroscopic analyses.					
31826501	6	59	theme	filtration	957:966	arg1	Moreovers					942:950	Moreovers	942:950	Moreovers	942:950	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	6	59	theme	filtration	957:966	arg1	chromatography					968:981	gel filtration chromatography	953:981	gel filtration chromatography	953:981	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	1	60	theme	fennel	123:128	arg1	wastes					130:135	lemon and fennel wastes	113:135	lemon and fennel wastes	113:135	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	1	60	theme	fennel	123:128	arg1	sources					193:199	secondary-raw polysaccharide sources	164:199	secondary-raw polysaccharide sources	164:199	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	5	61	theme	FTIR	913:916	arg1	analyses					932:939	FTIR spectroscopic analyses	913:939	FTIR spectroscopic analyses	913:939	These findings were confirmed by NMR and FTIR spectroscopic analyses.					
31826501	3	62	theme	high	615:618	arg1	yield					620:624	high yield	615:624	high yield of lemon and fennel polysaccharides (LP and FP)	615:672	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	1	63	used	used	156:159	arg2	sources					193:199	secondary-raw polysaccharide sources	164:199	secondary-raw polysaccharide sources	164:199	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	1	63	used	used	156:159	arg2	wastes					130:135	lemon and fennel wastes	113:135	lemon and fennel wastes	113:135	In this paper, lemon and fennel wastes were recovered and used as secondary-raw polysaccharide sources.					
31826501	2	64	from	application	399:409	arg1	way					448:450	a cost effective and eco-friendly way	414:450	a cost effective and eco-friendly way	414:450	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	6	65	theme	waste	1067:1071	arg1	fraction					1073:1080	FP waste fraction	1064:1080	FP waste fraction	1064:1080	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	2	66	theme	sodium	319:324	arg1	performances					335:346	sodium alginate performances	319:346	sodium alginate performances	319:346	These polysaccharides were exploited as natural plasticizers of sodium alginate (A) based films, in order to improve sodium alginate performances, limited by its fragility, extending its potential application in a cost effective and eco-friendly way.					
31826501	0	67	theme	sodium	81:86	arg1	alginate					88:95	sodium alginate	81:95	sodium alginate	81:95	Vegetable wastes derived polysaccharides as natural eco-friendly plasticizers of sodium alginate.					
31826501	8	68	theme	ALP	1438:1440	arg1	films					1442:1446	AFP and ALP films	1430:1446	AFP and ALP films	1430:1446	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
31826501	4	69	theme	xyslose	737:743	arg1	presence					725:732	the presence	721:732	the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains	721:869	Actually, HPAE-PAD and TLC analyses evidenced the presence of xyslose, galactose, glucose and rhamnose monomers and galacturonic acid, typical of polysaccharides like pectin and xyloglucan chains.					
31826501	3	70	theme	assisted	517:524	arg1	UAE					538:540	UAE	538:540	UAE	538:540	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	3	70	theme	assisted	517:524	arg1	extraction					526:535	ultrasound assisted extraction	506:535	ultrasound assisted extraction (UAE)	506:541	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	6	71	theme	FP	1064:1065	arg1	fraction					1073:1080	FP waste fraction	1064:1080	FP waste fraction	1064:1080	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	6	72	theme	recovered	1021:1029	arg1	polysaccharides					1031:1045	recovered polysaccharides	1021:1045	recovered polysaccharides	1021:1045	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	6	73	theme	gel	953:955	arg1	Moreovers					942:950	Moreovers	942:950	Moreovers	942:950	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	6	73	theme	gel	953:955	arg1	chromatography					968:981	gel filtration chromatography	953:981	gel filtration chromatography	953:981	Moreovers, gel filtration chromatography assessed the high molecular weight of recovered polysaccharides, particularly of FP waste fraction.					
31826501	3	74	theme	polysaccharides	646:660	arg1	yield					620:624	high yield	615:624	high yield of lemon and fennel polysaccharides (LP and FP)	615:672	Different green processes, such as maceration (MAC), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), were carried out for obtaining high yield of lemon and fennel polysaccharides (LP and FP).					
31826501	8	75	theme	films	1442:1446	arg1	kinetics					1418:1425	a faster degradation kinetics	1397:1425	a faster degradation kinetics of AFP and ALP films	1397:1446	DSC analysis evidenced a significant decreasing of glass transition temperature of the polymer, tensile tests showed an enlightened rising of elongation at break and TGA analysis showed a faster degradation kinetics of AFP and ALP films, as expected in a plasticized system.					
29420466	7	0	theme	cell	1478:1481	arg1	walls					1483:1487	cell walls	1478:1487	cell walls	1478:1487	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	2	1	theme	Natural	353:359	arg1	wood					361:364	Natural wood	353:364	Natural wood	353:364	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	1	theme	Natural	353:359	arg1	material					393:400	a low-cost and abundant material	369:400	a low-cost and abundant material	369:400	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	1	theme	Natural	353:359	arg1	material					450:457	a structural material	437:457	a structural material for building and furniture construction	437:497	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	6	2	with	material	1125:1132	arg1	stability					1245:1253	greater dimensional stability	1225:1253	greater dimensional stability	1225:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	6	2	with	material	1125:1132	arg1	increase					1159:1166	a more than tenfold increase	1139:1166	a more than tenfold increase in strength, toughness and ballistic resistance	1139:1214	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	9	3	dep	low-cost	1772:1779	arg1	lightweight					1800:1810	lightweight	1800:1810	lightweight	1800:1810	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	9	3	dep	low-cost	1772:1779	arg1	high-performance					1782:1797	high-performance	1782:1797	high-performance	1782:1797	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	3	4	theme	wood	547:550	arg1	performance					524:534	the mechanical performance	509:534	the mechanical performance of natural wood (its strength and toughness)	509:579	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	3	4	theme	wood	547:550	arg1	unsatisfactory					584:597	unsatisfactory	584:597	unsatisfactory	584:597	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	7	5	theme	Na2SO3	1412:1417	arg1	mixture					1392:1398	an aqueous mixture	1381:1398	an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing	1381:1442	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	9	6	theme	processed	1666:1674	arg1	wood					1676:1679	Our processed wood	1662:1679	Our processed wood	1662:1679	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	7	7	theme	NaOH	1403:1406	arg1	mixture					1392:1398	an aqueous mixture	1381:1398	an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing	1381:1442	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	6	8	dep	tenfold	1151:1157	arg1	than					1146:1149	than	1146:1149	than	1146:1149	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	6	8	dep	tenfold	1151:1157	arg1	more					1141:1144	more	1141:1144	more	1141:1144	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	5	9	theme	dimensional	878:888	arg1	stability					890:898	lack dimensional stability	873:898	lack dimensional stability	873:898	However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken.					
29420466	1	10	with	materials	95:103	arg1	performance					133:143	exceptional mechanical performance	110:143	exceptional mechanical performance	110:143	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	9	11	theme	specific	1687:1694	arg1	strength					1696:1703	a specific strength	1685:1703	a specific strength higher than that of most structural metals and alloys	1685:1757	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	6	12	theme	dimensional	1233:1243	arg1	stability					1245:1253	greater dimensional stability	1225:1253	greater dimensional stability	1225:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	7	13	with	wood	1535:1538	arg1	nanofibres					1570:1579	highly aligned cellulose nanofibres	1545:1579	highly aligned cellulose nanofibres	1545:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	5	14	theme	incomplete	844:853	arg1	densification					855:867	incomplete densification	844:867	incomplete densification	844:867	However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken.					
29420466	9	15	theme	higher	1705:1710	arg1	strength					1696:1703	a specific strength	1685:1703	a specific strength higher than that of most structural metals and alloys	1685:1757	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	9	16	dep	it	1767:1768	arg1	alternative					1812:1822	a low-cost, high-performance, lightweight alternative	1770:1822	it a low-cost, high-performance, lightweight alternative	1767:1822	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	2	17	used	used	415:418	arg2	wood					361:364	Natural wood	353:364	Natural wood	353:364	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	17	used	used	415:418	arg2	material					393:400	a low-cost and abundant material	369:400	a low-cost and abundant material	369:400	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	17	used	used	415:418	arg2	material					450:457	a structural material	437:457	a structural material for building and furniture construction	437:497	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	3	18	theme	engineering	617:627	arg1	structures					629:638	many advanced engineering structures	603:638	many advanced engineering structures	603:638	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	3	19	dep	wood	547:550	arg1	toughness					570:578	toughness	570:578	toughness	570:578	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	3	19	dep	wood	547:550	arg1	strength					557:564	its strength	553:564	its strength	553:564	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	9	20	theme	most	1725:1728	arg1	metals					1741:1746	most structural metals	1725:1746	most structural metals	1725:1746	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	1	21	theme	biomimetic	329:338	arg1	processes					268:276	complex manufacturing processes	246:276	complex manufacturing processes	246:276	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	1	21	theme	biomimetic	329:338	arg1	composites					340:349	polymer-based and biomimetic composites	311:349	polymer-based and biomimetic composites	311:349	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	5	22	theme	existing	817:824	arg1	methods					826:832	the existing methods	813:832	the existing methods	813:832	However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken.					
29420466	1	23	theme	adverse	181:187	arg1	impact					203:208	adverse environmental impact	181:208	adverse environmental impact	181:208	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	24	theme	aligned	1552:1558	arg1	nanofibres					1570:1579	highly aligned cellulose nanofibres	1545:1579	highly aligned cellulose nanofibres	1545:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	6	25	theme	ballistic	1195:1203	arg1	resistance					1205:1214	ballistic resistance	1195:1214	ballistic resistance	1195:1214	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	4	26	theme	enhanced	755:762	arg1	performance					775:785	the enhanced mechanical performance	751:785	the enhanced mechanical performance of natural wood	751:801	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	7	27	from	wood	1351:1354	arg1	removal					1298:1304	the partial removal	1286:1304	the partial removal of lignin and hemicellulose from the natural wood	1286:1354	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	6	28	from	increase	1159:1166	arg1	toughness					1181:1189	toughness	1181:1189	toughness	1181:1189	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	6	28	from	increase	1159:1166	arg1	resistance					1205:1214	ballistic resistance	1195:1214	ballistic resistance	1195:1214	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	6	28	from	increase	1159:1166	arg1	strength					1171:1178	strength	1171:1178	strength	1171:1178	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	1	29	theme	structural	84:93	arg1	materials					95:103	Synthetic structural materials	74:103	Synthetic structural materials with exceptional mechanical performance	74:143	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	30	theme	natural	1343:1349	arg1	wood					1351:1354	the natural wood	1339:1354	the natural wood	1339:1354	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	0	31	theme	Processing	0:9	arg1	wood					24:27	Processing bulk natural wood	0:27	Processing bulk natural wood into a high-performance structural material	0:71	Processing bulk natural wood into a high-performance structural material.					
29420466	1	32	theme	high	287:290	arg1	cost					292:295	thus high cost	282:295	thus high cost	282:295	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	33	from	process	1370:1376	arg1	mixture					1392:1398	an aqueous mixture	1381:1398	an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing	1381:1442	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	0	34	theme	natural	16:22	arg1	wood					24:27	Processing bulk natural wood	0:27	Processing bulk natural wood into a high-performance structural material	0:71	Processing bulk natural wood into a high-performance structural material.					
29420466	1	35	theme	mechanical	122:131	arg1	performance					133:143	exceptional mechanical performance	110:143	exceptional mechanical performance	110:143	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	6	36	theme	effective	1031:1039	arg1	strategy					1041:1048	a simple and effective strategy	1018:1048	a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability	1018:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	7	37	theme	densification	1506:1518	arg1	collapse					1466:1473	the total collapse	1456:1473	the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres	1456:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	6	38	theme	simple	1020:1025	arg1	strategy					1041:1048	a simple and effective strategy	1018:1048	a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability	1018:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	4	39	with	Pre-treatment	658:670	arg1	ammonia					690:696	ammonia	690:696	ammonia	690:696	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	4	39	with	Pre-treatment	658:670	arg1	heat					684:687	heat	684:687	heat	684:687	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	4	39	with	Pre-treatment	658:670	arg1	steam					677:681	steam	677:681	steam	677:681	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	4	39	with	Pre-treatment	658:670	arg1	cold					701:704	cold	701:704	cold	701:704	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	0	40	theme	high-performance	36:51	arg1	material					64:71	a high-performance structural material	34:71	a high-performance structural material	34:71	Processing bulk natural wood into a high-performance structural material.					
29420466	1	41	theme	polymer-based	311:323	arg1	processes					268:276	complex manufacturing processes	246:276	complex manufacturing processes	246:276	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	1	41	theme	polymer-based	311:323	arg1	composites					340:349	polymer-based and biomimetic composites	311:349	polymer-based and biomimetic composites	311:349	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	42	theme	partial	1290:1296	arg1	removal					1298:1304	the partial removal	1286:1304	the partial removal of lignin and hemicellulose from the natural wood	1286:1354	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	3	43	theme	many	603:606	arg1	structures					629:638	many advanced engineering structures	603:638	many advanced engineering structures	603:638	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	4	44	theme	natural	790:796	arg1	wood					798:801	natural wood	790:801	natural wood	790:801	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	8	45	theme	various	1637:1643	arg1	species					1645:1651	various species	1637:1651	various species of wood	1637:1659	This strategy is shown to be universally effective for various species of wood.					
29420466	7	46	theme	walls	1483:1487	arg1	collapse					1466:1473	the total collapse	1456:1473	the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres	1456:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	4	47	dep	steam	677:681	arg1	rolling					706:712	rolling	706:712	rolling	706:712	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	6	48	theme	high-performance	1097:1112	arg1	material					1125:1132	a high-performance structural material	1095:1132	a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability	1095:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	7	49	theme	two-step	1260:1267	arg1	process					1269:1275	Our two-step process	1256:1275	Our two-step process	1256:1275	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	1	50	theme	manufacturing	254:266	arg1	processes					268:276	complex manufacturing processes	246:276	complex manufacturing processes	246:276	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	1	50	theme	manufacturing	254:266	arg1	composites					340:349	polymer-based and biomimetic composites	311:349	polymer-based and biomimetic composites	311:349	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	51	theme	total	1460:1464	arg1	collapse					1466:1473	the total collapse	1456:1473	the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres	1456:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	3	52	theme	natural	539:545	arg1	wood					547:550	natural wood	539:550	natural wood (its strength and toughness)	539:579	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	6	53	theme	tenfold	1151:1157	arg1	increase					1159:1166	a more than tenfold increase	1139:1166	a more than tenfold increase in strength, toughness and ballistic resistance	1139:1214	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	1	54	theme	environmental	189:201	arg1	impact					203:208	adverse environmental impact	181:208	adverse environmental impact	181:208	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	5	55	theme	lack	873:876	arg1	stability					890:898	lack dimensional stability	873:898	lack dimensional stability	873:898	However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken.					
29420466	7	56	theme	aqueous	1384:1390	arg1	mixture					1392:1398	an aqueous mixture	1381:1398	an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing	1381:1442	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	3	57	theme	advanced	608:615	arg1	structures					629:638	many advanced engineering structures	603:638	many advanced engineering structures	603:638	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	5	58	theme	humid	929:933	arg1	environments					935:946	humid environments	929:946	humid environments	929:946	However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken.					
29420466	7	59	theme	boiling	1362:1368	arg1	process					1370:1376	a boiling process	1360:1376	a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing	1360:1442	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	1	60	theme	large	164:168	arg1	weight					170:175	large weight	164:175	large weight	164:175	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	61	theme	cellulose	1560:1568	arg1	nanofibres					1570:1579	highly aligned cellulose nanofibres	1545:1579	highly aligned cellulose nanofibres	1545:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	9	62	theme	structural	1730:1739	arg1	metals					1741:1746	most structural metals	1725:1746	most structural metals	1725:1746	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	2	63	dep	building	463:470	arg1	construction					486:497	construction	486:497	construction	486:497	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	7	64	theme	wood	1535:1538	arg1	walls					1483:1487	cell walls	1478:1487	cell walls	1478:1487	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	7	64	theme	wood	1535:1538	arg1	densification					1506:1518	the complete densification	1493:1518	the complete densification of the natural wood with highly aligned cellulose nanofibres	1493:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	1	65	theme	Synthetic	74:82	arg1	materials					95:103	Synthetic structural materials	74:103	Synthetic structural materials with exceptional mechanical performance	74:143	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	6	66	theme	natural	1068:1074	arg1	wood					1076:1079	bulk natural wood	1063:1079	bulk natural wood	1063:1079	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	4	67	theme	mechanical	764:773	arg1	performance					775:785	the enhanced mechanical performance	751:785	the enhanced mechanical performance of natural wood	751:801	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	2	68	theme	abundant	384:391	arg1	wood					361:364	Natural wood	353:364	Natural wood	353:364	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	68	theme	abundant	384:391	arg1	material					393:400	a low-cost and abundant material	369:400	a low-cost and abundant material	369:400	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	0	69	theme	bulk	11:14	arg1	wood					24:27	Processing bulk natural wood	0:27	Processing bulk natural wood into a high-performance structural material	0:71	Processing bulk natural wood into a high-performance structural material.					
29420466	7	70	theme	hemicellulose	1320:1332	arg1	removal					1298:1304	the partial removal	1286:1304	the partial removal of lignin and hemicellulose from the natural wood	1286:1354	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	9	71	theme	low-cost	1772:1779	arg1	alternative					1812:1822	a low-cost, high-performance, lightweight alternative	1770:1822	it a low-cost, high-performance, lightweight alternative	1767:1822	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	2	72	theme	low-cost	371:378	arg1	wood					361:364	Natural wood	353:364	Natural wood	353:364	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	72	theme	low-cost	371:378	arg1	material					393:400	a low-cost and abundant material	369:400	a low-cost and abundant material	369:400	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	1	73	theme	exceptional	110:120	arg1	performance					133:143	exceptional mechanical performance	110:143	exceptional mechanical performance	110:143	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	74	theme	lignin	1309:1314	arg1	removal					1298:1304	the partial removal	1286:1304	the partial removal of lignin and hemicellulose from the natural wood	1286:1354	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	1	75	dep	weight	170:175	arg1	alloys					235:240	alloys	235:240	alloys	235:240	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	1	75	dep	weight	170:175	arg1	steels					224:229	steels	224:229	steels	224:229	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	7	76	theme	natural	1527:1533	arg1	wood					1535:1538	the natural wood	1523:1538	the natural wood with highly aligned cellulose nanofibres	1523:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	0	77	theme	structural	53:62	arg1	material					64:71	a high-performance structural material	34:71	a high-performance structural material	34:71	Processing bulk natural wood into a high-performance structural material.					
29420466	2	78	theme	structural	439:448	arg1	material					450:457	a structural material	437:457	a structural material for building and furniture construction	437:497	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	2	78	theme	structural	439:448	arg1	wood					361:364	Natural wood	353:364	Natural wood	353:364	Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction.					
29420466	7	79	theme	complete	1497:1504	arg1	densification					1506:1518	the complete densification	1493:1518	the complete densification of the natural wood with highly aligned cellulose nanofibres	1493:1579	Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres.					
29420466	6	80	theme	greater	1225:1231	arg1	stability					1245:1253	greater dimensional stability	1225:1253	greater dimensional stability	1225:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	6	81	theme	structural	1114:1123	arg1	material					1125:1132	a high-performance structural material	1095:1132	a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability	1095:1253	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	9	82	contain	has	1681:1683	arg2	strength					1696:1703	a specific strength	1685:1703	a specific strength higher than that of most structural metals and alloys	1685:1757	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	9	82	contain	has	1681:1683	arg1	wood					1676:1679	Our processed wood	1662:1679	Our processed wood	1662:1679	Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.					
29420466	8	83	theme	wood	1656:1659	arg1	species					1645:1651	various species	1637:1651	various species of wood	1637:1659	This strategy is shown to be universally effective for various species of wood.					
29420466	6	84	theme	bulk	1063:1066	arg1	wood					1076:1079	bulk natural wood	1063:1079	bulk natural wood	1063:1079	Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability.					
29420466	4	85	theme	wood	798:801	arg1	performance					775:785	the enhanced mechanical performance	751:785	the enhanced mechanical performance of natural wood	751:801	Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood.					
29420466	3	86	theme	mechanical	513:522	arg1	performance					524:534	the mechanical performance	509:534	the mechanical performance of natural wood (its strength and toughness)	509:579	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	3	86	theme	mechanical	513:522	arg1	unsatisfactory					584:597	unsatisfactory	584:597	unsatisfactory	584:597	However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications.					
29420466	1	87	theme	complex	246:252	arg1	processes					268:276	complex manufacturing processes	246:276	complex manufacturing processes	246:276	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
29420466	1	87	theme	complex	246:252	arg1	composites					340:349	polymer-based and biomimetic composites	311:349	polymer-based and biomimetic composites	311:349	Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites).					
31556417	5	0	theme	plaque	1149:1154	arg1	area					1156:1159	the atherosclerotic plaque area	1129:1159	the atherosclerotic plaque area	1129:1159	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	7	1	theme	CCPS	1525:1528	arg1	related					1572:1578	related	1572:1578	related	1572:1578	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	7	1	theme	CCPS	1525:1528	arg1	effects					1514:1520	the preventive effects	1499:1520	the preventive effects of CCPS on AS in mice induced by HFD	1499:1557	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	7	2	theme	microbiota	1616:1625	arg1	composition					1597:1607	the composition	1593:1607	the composition of gut microbiota	1593:1625	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	0	3	from	activities	11:20	arg1	chinensis					62:70	chinensis	62:70	chinensis	62:70	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	5	4	theme	lipid	1064:1068	arg1	balance					1070:1076	the plasma lipid balance	1053:1076	the plasma lipid balance	1053:1076	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	4	5	theme	groups	883:888	arg1	compositions					850:861	gut microbiota compositions	835:861	gut microbiota compositions	835:861	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	4	5	theme	groups	883:888	arg1	characteristics					791:805	the histopathological characteristics	769:805	the histopathological characteristics	769:805	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	4	5	theme	groups	883:888	arg1	parameters					820:829	biochemical parameters	808:829	biochemical parameters	808:829	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	6	6	theme	Mechanistic	1189:1199	arg1	analysis					1201:1208	Mechanistic analysis	1189:1208	Mechanistic analysis	1189:1208	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	7	7	theme	preventive	1503:1512	arg1	related					1572:1578	related	1572:1578	related	1572:1578	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	7	7	theme	preventive	1503:1512	arg1	effects					1514:1520	the preventive effects	1499:1520	the preventive effects of CCPS on AS in mice induced by HFD	1499:1557	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	7	8	from	effects	1514:1520	arg1	AS					1533:1534	AS	1533:1534	AS	1533:1534	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	0	9	theme	gut	133:135	arg1	microbiota					137:146	gut microbiota	133:146	gut microbiota in ApoE-deficient mice	133:169	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	4	10	theme	experimental	870:881	arg1	groups					883:888	the experimental groups	866:888	the experimental groups	866:888	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	7	11	theme	gut	1612:1614	arg1	microbiota					1616:1625	gut microbiota	1612:1625	gut microbiota	1612:1625	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	0	12	theme	ApoE-deficient	151:164	arg1	mice					166:169	ApoE-deficient mice	151:169	ApoE-deficient mice	151:169	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	0	13	from	chinensis	62:70	arg1	polysaccharides					25:39	polysaccharides	25:39	polysaccharides from Cipangopaludina chinensis	25:70	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	0	13	from	chinensis	62:70	arg1	activities					11:20	Protective activities	0:20	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis	0:116	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	4	14	theme	gut	835:837	arg1	compositions					850:861	gut microbiota compositions	835:861	gut microbiota compositions	835:861	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	6	15	theme	bacteria	1411:1418	arg1	abundance					1387:1395	the abundance	1383:1395	the abundance of beneficial bacteria	1383:1418	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	3	16	theme	blank	705:709	arg1	solvent					711:717	a blank solvent	703:717	a blank solvent	703:717	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	6	17	theme	Firmicutes/Bacteroidetes	1297:1320	arg1	ratio					1288:1292	the ratio	1284:1292	the ratio of Firmicutes/Bacteroidetes	1284:1320	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	0	18	theme	Protective	0:9	arg1	activities					11:20	Protective activities	0:20	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis	0:116	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	1	19	dep	atherosclerosis	355:369	arg1	AS					372:373	AS	372:373	AS	372:373	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	5	20	theme	CCPS	939:942	arg1	supplementation					944:958	the CCPS supplementation	935:958	the CCPS supplementation	935:958	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	2	21	theme	preventive	454:463	arg1	effects					465:471	the preventive effects	450:471	the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation	450:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	3	22	theme	normal	658:663	arg1	diet					665:668	HFD or normal diet	651:668	HFD or normal diet	651:668	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	0	23	theme	polysaccharides	25:39	arg1	activities					11:20	Protective activities	0:20	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis	0:116	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	4	24	theme	histopathological	773:789	arg1	characteristics					791:805	the histopathological characteristics	769:805	the histopathological characteristics	769:805	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	6	25	theme	beneficial	1400:1409	arg1	bacteria					1411:1418	beneficial bacteria	1400:1418	beneficial bacteria	1400:1418	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	7	26	theme	environmental	1442:1454	arg1	analysis					1475:1482	environmental factor correlation analysis	1442:1482	environmental factor correlation analysis	1442:1482	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	1	27	contain	have	244:247	arg2	effect					270:275	a certain protective effect	249:275	a certain protective effect on cardiovascular diseases	249:302	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	1	27	contain	have	244:247	arg2	slow					326:329	slow	326:329	slow	326:329	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	1	27	contain	have	244:247	arg1	polysaccharides					181:195	Sulfated polysaccharides	172:195	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis	172:242	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	1	27	contain	have	244:247	arg1	CCPS					198:201	CCPS	198:201	CCPS	198:201	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	1	28	theme	certain	251:257	arg1	effect					270:275	a certain protective effect	249:275	a certain protective effect on cardiovascular diseases	249:302	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	2	29	theme	underlying	531:540	arg1	mechanism					542:550	the underlying mechanism	527:550	the underlying mechanism focusing on gut microbiota profile modulation	527:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	3	30	theme	HFD	651:653	arg1	diet					665:668	HFD or normal diet	651:668	HFD or normal diet	651:668	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	7	31	theme	factor	1456:1461	arg1	analysis					1475:1482	environmental factor correlation analysis	1442:1482	environmental factor correlation analysis	1442:1482	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	1	32	theme	protective	259:268	arg1	effect					270:275	a certain protective effect	249:275	a certain protective effect on cardiovascular diseases	249:302	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	2	33	from	-induced	503:510	arg1	mice					518:521	mice	518:521	mice	518:521	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	7	34	theme	correlation	1463:1473	arg1	analysis					1475:1482	environmental factor correlation analysis	1442:1482	environmental factor correlation analysis	1442:1482	Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.					
31556417	2	35	theme	CCPS	476:479	arg1	effects					465:471	the preventive effects	450:471	the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation	450:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	1	36	theme	atherosclerosis	355:369	arg1	development					340:350	the development	336:350	the development of atherosclerosis (AS)	336:374	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	2	37	theme	profile	579:585	arg1	modulation					587:596	gut microbiota profile modulation	564:596	gut microbiota profile modulation	564:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	5	38	theme	atherosclerotic	1094:1108	arg1	index					1110:1114	the atherosclerotic index	1090:1114	the atherosclerotic index	1090:1114	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	1	39	from	effect	270:275	arg1	diseases					295:302	cardiovascular diseases	280:302	cardiovascular diseases	280:302	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	6	40	dep	regulated	1229:1237	arg1	increasing					1372:1381	increasing	1372:1381	increasing the abundance of beneficial bacteria	1372:1418	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	6	40	dep	regulated	1229:1237	arg1	reducing					1275:1282	reducing	1275:1282	reducing the ratio of Firmicutes/Bacteroidetes	1275:1320	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	6	40	dep	regulated	1229:1237	arg1	decreasing					1323:1332	decreasing	1323:1332	decreasing the abundance of harmful bacteria	1323:1366	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	1	41	dep	work	389:392	arg1	unclear					404:410	unclear	404:410	unclear	404:410	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	2	42	theme	microbiota	568:577	arg1	modulation					587:596	gut microbiota profile modulation	564:596	gut microbiota profile modulation	564:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	4	43	theme	biochemical	808:818	arg1	parameters					820:829	biochemical parameters	808:829	biochemical parameters	808:829	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	2	44	from	effects	465:471	arg1	mechanism					542:550	the underlying mechanism	527:550	the underlying mechanism focusing on gut microbiota profile modulation	527:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	2	44	from	effects	465:471	arg1	HFD					499:501	HFD	499:501	HFD	499:501	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	2	44	from	effects	465:471	arg1	high-fat-diet					484:496	high-fat-diet	484:496	high-fat-diet (HFD)-induced AS in mice	484:521	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	2	45	theme	gut	564:566	arg1	modulation					587:596	gut microbiota profile modulation	564:596	gut microbiota profile modulation	564:596	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	5	46	theme	plasma	1057:1062	arg1	balance					1070:1076	the plasma lipid balance	1053:1076	the plasma lipid balance	1053:1076	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	3	47	theme	E	626:626	arg1	mice					637:640	male apolipoprotein E knockout mice	606:640	male apolipoprotein E knockout mice	606:640	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	1	48	theme	Sulfated	172:179	arg1	CCPS					198:201	CCPS	198:201	CCPS	198:201	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	1	48	theme	Sulfated	172:179	arg1	polysaccharides					181:195	Sulfated polysaccharides	172:195	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis	172:242	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	6	49	theme	bacteria	1359:1366	arg1	abundance					1338:1346	the abundance	1334:1346	the abundance of harmful bacteria	1334:1366	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	6	50	theme	harmful	1351:1357	arg1	bacteria					1359:1366	harmful bacteria	1351:1366	harmful bacteria	1351:1366	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	0	51	from	microbiota	137:146	arg1	mice					166:169	ApoE-deficient mice	151:169	ApoE-deficient mice	151:169	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	3	52	theme	knockout	628:635	arg1	mice					637:640	male apolipoprotein E knockout mice	606:640	male apolipoprotein E knockout mice	606:640	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	1	53	theme	cardiovascular	280:293	arg1	diseases					295:302	cardiovascular diseases	280:302	cardiovascular diseases	280:302	Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear.					
31556417	2	54	theme	-induced	503:510	arg1	HFD					499:501	HFD	499:501	HFD	499:501	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	2	54	theme	-induced	503:510	arg1	high-fat-diet					484:496	high-fat-diet	484:496	high-fat-diet (HFD)-induced AS in mice	484:521	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	2	55	from	mice	518:521	arg1	-induced					503:510	-induced	503:510	-induced	503:510	In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation.					
31556417	3	56	theme	male	606:609	arg1	mice					637:640	male apolipoprotein E knockout mice	606:640	male apolipoprotein E knockout mice	606:640	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	0	57	theme	high-fat-diet-induced	80:100	arg1	atherosclerosis					102:116	high-fat-diet-induced atherosclerosis	80:116	high-fat-diet-induced atherosclerosis	80:116	Protective activities of polysaccharides from Cipangopaludina chinensis against high-fat-diet-induced atherosclerosis via regulating gut microbiota in ApoE-deficient mice.					
31556417	5	58	theme	atherosclerotic	1133:1147	arg1	area					1156:1159	the atherosclerotic plaque area	1129:1159	the atherosclerotic plaque area	1129:1159	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
31556417	3	59	theme	apolipoprotein	611:624	arg1	mice					637:640	male apolipoprotein E knockout mice	606:640	male apolipoprotein E knockout mice	606:640	First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks.					
31556417	4	60	theme	microbiota	839:848	arg1	compositions					850:861	gut microbiota compositions	835:861	gut microbiota compositions	835:861	Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared.					
31556417	6	61	theme	bacterial	1243:1251	arg1	composition					1253:1263	the bacterial composition	1239:1263	the bacterial composition including	1239:1273	Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.					
31556417	5	62	theme	dose-dependent	1166:1179	arg1	manner					1181:1186	a dose-dependent manner	1164:1186	a dose-dependent manner	1164:1186	The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner.					
29726707	2	0	theme	compound	477:484	arg1	concentration					494:506	compound enzymes concentration	477:506	compound enzymes concentration	477:506	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	0	1	from	purification	12:23	arg1	officinale					91:100	Dendrobium officinale	80:100	Dendrobium officinale	80:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	1	2	theme	Dendrobium	261:270	arg1	officinale					272:281	Dendrobium officinale	261:281	Dendrobium officinale	261:281	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	0	3	theme	Dendrobium	80:89	arg1	officinale					91:100	Dendrobium officinale	80:100	Dendrobium officinale	80:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	1	4	theme	Sephadex	345:352	arg1	G75					354:356	Sephadex G75	345:356	Sephadex G75	345:356	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	5	5	theme	antioxidant	1055:1065	arg1	activity					1067:1074	considerable antioxidant activity	1042:1074	considerable antioxidant activity	1042:1074	Simultaneously, the two fractions possessed considerable antioxidant activity.					
29726707	4	6	contain	had	887:889	arg2	backbone					903:910	a similarly backbone	891:910	a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp	891:974	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	4	6	contain	had	887:889	arg1	polysaccharides					871:885	both polysaccharides	866:885	both polysaccharides	866:885	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	4	7	theme	DOP2-DES	761:768	arg1	features					736:743	Structural features	725:743	Structural features of DOP1-DES and DOP2-DES	725:768	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	0	8	theme	deep	105:108	arg1	solvents					119:126	deep eutectic solvents	105:126	deep eutectic solvents	105:126	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	9	from	Extraction	0:9	arg1	officinale					91:100	Dendrobium officinale	80:100	Dendrobium officinale	80:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	2	10	theme	extraction	515:524	arg1	yield					526:530	the extraction yield	511:530	the extraction yield of polysaccharides	511:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	3	11	theme	3.7:1	704:708	arg1	ratio					685:689	the ratio	681:689	the ratio of 2.2:1 and 3.7:1, respectively	681:722	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	1	12	dep	polysaccharides	139:153	arg1	polysaccharides					139:153	Two novel polysaccharides DOP1-DES and DOP2-DES	129:175	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da	129:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	1	12	dep	polysaccharides	139:153	arg1	DOP2-DES					168:175	DOP2-DES	168:175	DOP2-DES	168:175	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	1	12	dep	polysaccharides	139:153	arg1	DOP1-DES					155:162	DOP1-DES	155:162	DOP1-DES	155:162	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	1	13	theme	Sephadex	362:369	arg1	chromatography					376:389	Sephadex G200 chromatography	362:389	Sephadex G200 chromatography	362:389	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	2	14	theme	extraction	415:424	arg1	temperature					426:436	extraction temperature	415:436	extraction temperature	415:436	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	1	15	theme	molecular	182:190	arg1	weight					192:197	molecular weight	182:197	molecular weight of 297,911 Da and 30,400 Da	182:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	0	16	theme	eutectic	110:117	arg1	solvents					119:126	deep eutectic solvents	105:126	deep eutectic solvents	105:126	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	17	from	activity	41:48	arg1	officinale					91:100	Dendrobium officinale	80:100	Dendrobium officinale	80:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	1	18	with	polysaccharides	139:153	arg1	weight					192:197	molecular weight	182:197	molecular weight of 297,911 Da and 30,400 Da	182:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	4	19	theme	-linked	932:938	arg1	β-D-Manp					940:947	(1, 4)-linked β-D-Manp	926:947	β-D-Manp	940:947	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	2	20	theme	reaction	459:466	arg1	time					468:471	reaction time	459:471	reaction time	459:471	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	3	21	with	D-glucose	652:660	arg1	ratio					685:689	the ratio	681:689	the ratio of 2.2:1 and 3.7:1, respectively	681:722	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	4	22	link	-linked	932:938	arg1	β-D-Manp					940:947	(1, 4)-linked β-D-Manp	926:947	β-D-Manp	940:947	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	3	23	theme	composition	585:595	arg1	analysis					597:604	Monosaccharide composition analysis	570:604	Monosaccharide composition analysis	570:604	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	2	24	theme	ratio	445:449	arg1	effect					405:410	the effect	401:410	the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides	401:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	1	25	theme	first	291:295	arg1	time					297:300	the first time	287:300	the first time	287:300	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	5	26	theme	considerable	1042:1053	arg1	activity					1067:1074	considerable antioxidant activity	1042:1074	considerable antioxidant activity	1042:1074	Simultaneously, the two fractions possessed considerable antioxidant activity.					
29726707	1	27	theme	297,911 Da	202:211	arg1	weight					192:197	molecular weight	182:197	molecular weight of 297,911 Da and 30,400 Da	182:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	2	28	theme	molar	439:443	arg1	ratio					445:449	molar ratio	439:449	molar ratio of DES	439:456	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	4	29	link	-linked	959:965	arg1	β-D-Glcp					967:974	(1, 4)-linked β-D-Glcp	953:974	β-D-Glcp	967:974	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	3	30	dep	showed	606:611	arg1	composed					640:647	composed	640:647	showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively	606:722	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	3	31	with	D-mannose	666:674	arg1	ratio					685:689	the ratio	681:689	the ratio of 2.2:1 and 3.7:1, respectively	681:722	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	2	32	theme	temperature	426:436	arg1	effect					405:410	the effect	401:410	the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides	401:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	0	33	from	officinale	91:100	arg1	purification					12:23	purification	12:23	purification	12:23	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	33	from	officinale	91:100	arg1	activity					41:48	antioxidant activity	29:48	antioxidant activity	29:48	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	33	from	officinale	91:100	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	33	from	officinale	91:100	arg1	polysaccharides					59:73	novel polysaccharides	53:73	novel polysaccharides from Dendrobium officinale	53:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	3	34	theme	Monosaccharide	570:583	arg1	analysis					597:604	Monosaccharide composition analysis	570:604	Monosaccharide composition analysis	570:604	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	1	35	theme	30,400 Da	217:225	arg1	weight					192:197	molecular weight	182:197	molecular weight of 297,911 Da and 30,400 Da	182:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	2	36	theme	time	468:471	arg1	effect					405:410	the effect	401:410	the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides	401:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	2	37	theme	polysaccharides	535:549	arg1	yield					526:530	the extraction yield	511:530	the extraction yield of polysaccharides	511:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	4	38	theme	NMR	840:842	arg1	combination					793:803	a combination	791:803	a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio	791:995	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	0	39	theme	antioxidant	29:39	arg1	activity					41:48	antioxidant activity	29:48	antioxidant activity	29:48	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	4	40	theme	different	981:989	arg1	ratio					991:995	different ratio	981:995	different ratio	981:995	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	1	41	from	officinale	272:281	arg1	DESs					251:254	DESs	251:254	DESs from Dendrobium officinale for the first time	251:300	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	0	42	theme	novel	53:57	arg1	polysaccharides					59:73	novel polysaccharides	53:73	novel polysaccharides from Dendrobium officinale	53:100	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	2	43	theme	enzymes	486:492	arg1	concentration					494:506	compound enzymes concentration	477:506	compound enzymes concentration	477:506	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	1	44	theme	G200	371:374	arg1	chromatography					376:389	Sephadex G200 chromatography	362:389	Sephadex G200 chromatography	362:389	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	2	45	theme	DES	454:456	arg1	ratio					445:449	molar ratio	439:449	molar ratio of DES	439:456	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	2	45	theme	DES	454:456	arg1	time					468:471	reaction time	459:471	reaction time	459:471	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	2	45	theme	DES	454:456	arg1	concentration					494:506	compound enzymes concentration	477:506	compound enzymes concentration	477:506	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	2	45	theme	DES	454:456	arg1	temperature					426:436	extraction temperature	415:436	extraction temperature	415:436	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	1	46	theme	macroporous	321:331	arg1	resin					333:337	macroporous resin AB-8	321:342	macroporous resin AB-8	321:342	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	3	47	theme	2.2:1	694:698	arg1	ratio					685:689	the ratio	681:689	the ratio of 2.2:1 and 3.7:1, respectively	681:722	Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively.					
29726707	4	48	theme	Structural	725:734	arg1	features					736:743	Structural features	725:743	Structural features of DOP1-DES and DOP2-DES	725:768	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	1	49	theme	novel	133:137	arg1	polysaccharides					139:153	Two novel polysaccharides DOP1-DES and DOP2-DES	129:175	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da	129:225	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	1	49	theme	novel	133:137	arg1	DOP2-DES					168:175	DOP2-DES	168:175	DOP2-DES	168:175	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	1	49	theme	novel	133:137	arg1	DOP1-DES					155:162	DOP1-DES	155:162	DOP1-DES	155:162	Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography.					
29726707	4	50	theme	FT-IR	808:812	arg1	combination					793:803	a combination	791:803	a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio	791:995	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	4	51	theme	DOP1-DES	748:755	arg1	features					736:743	Structural features	725:743	Structural features of DOP1-DES and DOP2-DES	725:768	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	2	52	theme	concentration	494:506	arg1	effect					405:410	the effect	401:410	the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides	401:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	5	53	contain	possessed	1032:1040	arg2	activity					1067:1074	considerable antioxidant activity	1042:1074	considerable antioxidant activity	1042:1074	Simultaneously, the two fractions possessed considerable antioxidant activity.					
29726707	5	53	contain	possessed	1032:1040	arg1	fractions					1022:1030	the two fractions	1014:1030	the two fractions	1014:1030	Simultaneously, the two fractions possessed considerable antioxidant activity.					
29726707	4	54	theme	analysis	827:834	arg1	combination					793:803	a combination	791:803	a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio	791:995	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	2	55	from	effect	405:410	arg1	yield					526:530	the extraction yield	511:530	the extraction yield of polysaccharides	511:549	Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated.					
29726707	0	56	theme	polysaccharides	59:73	arg1	purification					12:23	purification	12:23	purification	12:23	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	56	theme	polysaccharides	59:73	arg1	activity					41:48	antioxidant activity	29:48	antioxidant activity	29:48	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	0	56	theme	polysaccharides	59:73	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, purification and antioxidant activity of novel polysaccharides from Dendrobium officinale by deep eutectic solvents.					
29726707	4	57	theme	-linked	959:965	arg1	β-D-Glcp					967:974	(1, 4)-linked β-D-Glcp	953:974	β-D-Glcp	967:974	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
29726707	4	58	theme	methylation	815:825	arg1	analysis					827:834	methylation analysis	815:834	methylation analysis	815:834	Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked β-D-Manp and (1, 4)-linked β-D-Glcp with different ratio.					
30515387	5	0	theme	tree	894:897	arg1	polysaccharides					909:923	tree peony pod polysaccharides	894:923	tree peony pod polysaccharides	894:923	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	0	1	theme	Peony	90:94	arg1	Pods					96:99	Tree Peony Pods	85:99	Tree Peony Pods	85:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	3	2	theme	experimental	651:662	arg1	yield					664:668	an experimental yield	648:668	an experimental yield of 14.14% ± 0.44%	648:686	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	4	3	theme	derivatization	844:857	arg1	method					859:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	3	4	theme	ultrasound	509:518	arg1	time					532:535	ultrasound irradiation time	509:535	ultrasound irradiation time	509:535	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	5	5	theme	molar	1040:1044	arg1	ratio					1046:1050	a molar ratio	1038:1050	a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21	1038:1116	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	0	6	from	Green	0:4	arg1	Pods					96:99	Tree Peony Pods	85:99	Tree Peony Pods	85:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	3	7	theme	irradiation	520:530	arg1	time					532:535	ultrasound irradiation time	509:535	ultrasound irradiation time	509:535	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	time					532:535	ultrasound irradiation time	509:535	ultrasound irradiation time	509:535	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	mL/g					596:599	25 mL/g	593:599	25 mL/g	593:599	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	W					506:506	250 W	502:506	250 W	502:506	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	g/mL					632:635	0.2 g/mL	628:635	0.2 g/mL	628:635	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	concentration					606:618	concentration	606:618	concentration of PEG	606:625	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	min					541:543	30 min	538:543	30 min; reaction temperature 50°C	538:570	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	8	dep	power	495:499	arg1	ratio					586:590	liquid-solid ratio	573:590	liquid-solid ratio	573:590	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	7	9	theme	pod	1311:1313	arg1	polysaccharides					1315:1329	the tree peony pod polysaccharides	1296:1329	the tree peony pod polysaccharides	1296:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	1	10	theme	polyethylene	279:290	arg1	solvent					312:318	the solvent	308:318	the solvent	308:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	1	10	theme	polyethylene	279:290	arg1	PEG					300:302	PEG	300:302	PEG	300:302	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	1	10	theme	polyethylene	279:290	arg1	glycol					292:297	polyethylene glycol	279:297	polyethylene glycol (PEG)	279:303	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	7	11	theme	moderate	1374:1381	arg1	activities					1395:1404	moderate antioxidant activities	1374:1404	moderate antioxidant activities	1374:1404	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	6	12	theme	tree	1123:1126	arg1	acid					1187:1190	galacturonic acid	1174:1190	galacturonic acid	1174:1190	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	12	theme	tree	1123:1126	arg1	polysaccharides					1138:1152	The tree peony pod polysaccharides	1119:1152	The tree peony pod polysaccharides obtained	1119:1161	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	4	13	theme	monosaccharide	707:720	arg1	composition					722:732	the monosaccharide composition	703:732	the monosaccharide composition of the tree peony pod polysaccharides	703:770	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	3	14	theme	%	686:686	arg1	yield					664:668	an experimental yield	648:668	an experimental yield of 14.14% ± 0.44%	648:686	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	15	theme	irradiation	483:493	arg1	power					495:499	ultrasound irradiation power	472:499	ultrasound irradiation power	472:499	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	15	theme	irradiation	483:493	arg1	conditions					460:469	Specific conditions	451:469	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL)	451:636	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	5	16	theme	1.44	1055:1058	arg1	ratio					1046:1050	a molar ratio	1038:1050	a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21	1038:1116	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	3	17	theme	ultrasound	472:481	arg1	power					495:499	ultrasound irradiation power	472:499	ultrasound irradiation power	472:499	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	17	theme	ultrasound	472:481	arg1	conditions					460:469	Specific conditions	451:469	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL)	451:636	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	0	18	from	Extraction	50:59	arg1	Pods					96:99	Tree Peony Pods	85:99	Tree Peony Pods	85:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	5	19	theme	galacturonic	972:983	arg1	acid					985:988	galacturonic acid	972:988	galacturonic acid	972:988	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	3	20	theme	Specific	451:458	arg1	power					495:499	ultrasound irradiation power	472:499	ultrasound irradiation power	472:499	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	20	theme	Specific	451:458	arg1	conditions					460:469	Specific conditions	451:469	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL)	451:636	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	21	theme	±	680:680	arg1	%					686:686	14.14% ± 0.44%	673:686	14.14% ± 0.44%	673:686	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	4	22	theme	precolumn	834:842	arg1	method					859:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	7	23	theme	polysaccharides	1315:1329	arg1	FRAP					1287:1290	FRAP	1287:1290	FRAP	1287:1290	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	23	theme	polysaccharides	1315:1329	arg1	DPPH					1278:1281	DPPH	1278:1281	DPPH	1278:1281	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	23	theme	polysaccharides	1315:1329	arg1	activities					1266:1275	the antioxidant activities	1250:1275	the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides	1250:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	24	theme	antioxidant	1383:1393	arg1	activities					1395:1404	moderate antioxidant activities	1374:1404	moderate antioxidant activities	1374:1404	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	4	25	theme	1-phenyl-3-methyl-5-pyrazolone	803:832	arg1	method					859:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method	801:864	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	1	26	theme	ultrasonic-assisted	188:206	arg1	method					219:224	an ultrasonic-assisted extraction method	185:224	an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent	185:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	0	27	theme	PEG-Based	20:28	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	28	theme	Antioxidant	129:139	arg1	Activity					141:148	Their Antioxidant Activity	123:148	Their Antioxidant Activity In Vitro	123:157	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	7	29	dep	activities	1266:1275	arg1	FRAP					1287:1290	FRAP	1287:1290	FRAP	1287:1290	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	29	dep	activities	1266:1275	arg1	DPPH					1278:1281	DPPH	1278:1281	DPPH	1278:1281	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	29	dep	activities	1266:1275	arg1	activities					1266:1275	the antioxidant activities	1250:1275	the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides	1250:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	3	30	theme	reaction	546:553	arg1	50°C					567:570	reaction temperature 50°C	546:570	30 min; reaction temperature 50°C	538:570	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	2	31	theme	polysaccharide	418:431	arg1	yield					444:448	the tree peony pod polysaccharide extraction yield	399:448	the tree peony pod polysaccharide extraction yield	399:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	2	32	theme	extraction	433:442	arg1	yield					444:448	the tree peony pod polysaccharide extraction yield	399:448	the tree peony pod polysaccharide extraction yield	399:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	0	33	from	Pods	96:99	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	33	from	Pods	96:99	arg1	Green					0:4	Green	0:4	Green	0:4	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	33	from	Pods	96:99	arg1	Evaluation					109:118	the Evaluation	105:118	the Evaluation of Their Antioxidant Activity In Vitro	105:157	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	33	from	Pods	96:99	arg1	Polysaccharides					64:78	Polysaccharides	64:78	Polysaccharides from Tree Peony Pods	64:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	3	34	theme	temperature	555:565	arg1	50°C					567:570	reaction temperature 50°C	546:570	30 min; reaction temperature 50°C	538:570	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	2	35	theme	pod	414:416	arg1	yield					444:448	the tree peony pod polysaccharide extraction yield	399:448	the tree peony pod polysaccharide extraction yield	399:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	5	36	theme	glucuronic	955:964	arg1	acid					966:969	glucuronic acid	955:969	glucuronic acid	955:969	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	1	37	from	pods	268:271	arg1	polysaccharides					236:250	polysaccharides	236:250	polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent	236:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	2	38	theme	peony	408:412	arg1	pod					414:416	the tree peony pod	399:416	the tree peony pod polysaccharide extraction yield	399:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	0	39	theme	Ultrasonic-Assisted	30:48	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	40	theme	Activity	141:148	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	40	theme	Activity	141:148	arg1	Green					0:4	Green	0:4	Green	0:4	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	40	theme	Activity	141:148	arg1	Evaluation					109:118	the Evaluation	105:118	the Evaluation of Their Antioxidant Activity In Vitro	105:157	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	7	41	theme	antioxidant	1254:1264	arg1	FRAP					1287:1290	FRAP	1287:1290	FRAP	1287:1290	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	41	theme	antioxidant	1254:1264	arg1	DPPH					1278:1281	DPPH	1278:1281	DPPH	1278:1281	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	41	theme	antioxidant	1254:1264	arg1	activities					1266:1275	the antioxidant activities	1250:1275	the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides	1250:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	7	42	theme	tree	1300:1303	arg1	polysaccharides					1315:1329	the tree peony pod polysaccharides	1296:1329	the tree peony pod polysaccharides	1296:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	5	43	contain	contained	925:933	arg2	arabinose					1011:1019	arabinose	1011:1019	arabinose	1011:1019	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg1	polysaccharides					909:923	tree peony pod polysaccharides	894:923	tree peony pod polysaccharides	894:923	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	mannoses					935:942	mannoses	935:942	mannoses	935:942	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	rhamnose					945:952	rhamnose	945:952	rhamnose	945:952	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	acid					985:988	galacturonic acid	972:988	galacturonic acid	972:988	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	acid					966:969	glucuronic acid	955:969	glucuronic acid	955:969	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	fucose					1026:1031	fucose	1026:1031	fucose	1026:1031	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	glucose					991:997	glucose	991:997	glucose	991:997	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	5	43	contain	contained	925:933	arg2	galactose					1000:1008	galactose	1000:1008	galactose	1000:1008	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	2	44	theme	tree	403:406	arg1	pod					414:416	the tree peony pod	399:416	the tree peony pod polysaccharide extraction yield	399:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	0	45	theme	Polysaccharides	64:78	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	45	theme	Polysaccharides	64:78	arg1	Green					0:4	Green	0:4	Green	0:4	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	0	45	theme	Polysaccharides	64:78	arg1	Evaluation					109:118	the Evaluation	105:118	the Evaluation of Their Antioxidant Activity In Vitro	105:157	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	3	46	theme	PEG	623:625	arg1	ratio					586:590	liquid-solid ratio	573:590	liquid-solid ratio	573:590	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	46	theme	PEG	623:625	arg1	mL/g					596:599	25 mL/g	593:599	25 mL/g	593:599	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	46	theme	PEG	623:625	arg1	concentration					606:618	concentration	606:618	concentration of PEG	606:625	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	47	theme	liquid-solid	573:584	arg1	ratio					586:590	liquid-solid ratio	573:590	liquid-solid ratio	573:590	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	3	48	theme	%	678:678	arg1	%					686:686	14.14% ± 0.44%	673:686	14.14% ± 0.44%	673:686	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	5	49	theme	peony	899:903	arg1	polysaccharides					909:923	tree peony pod polysaccharides	894:923	tree peony pod polysaccharides	894:923	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	6	50	theme	pod	1134:1136	arg1	acid					1187:1190	galacturonic acid	1174:1190	galacturonic acid	1174:1190	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	50	theme	pod	1134:1136	arg1	polysaccharides					1138:1152	The tree peony pod polysaccharides	1119:1152	The tree peony pod polysaccharides obtained	1119:1161	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	5	51	theme	pod	905:907	arg1	polysaccharides					909:923	tree peony pod polysaccharides	894:923	tree peony pod polysaccharides	894:923	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	4	52	theme	pod	752:754	arg1	polysaccharides					756:770	the tree peony pod polysaccharides	737:770	the tree peony pod polysaccharides	737:770	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	0	53	from	Evaluation	109:118	arg1	Pods					96:99	Tree Peony Pods	85:99	Tree Peony Pods	85:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	4	54	theme	peony	746:750	arg1	polysaccharides					756:770	the tree peony pod polysaccharides	737:770	the tree peony pod polysaccharides	737:770	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	6	55	theme	galacturonic	1174:1185	arg1	acid					1187:1190	galacturonic acid	1174:1190	galacturonic acid	1174:1190	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	55	theme	galacturonic	1174:1185	arg1	polysaccharides					1138:1152	The tree peony pod polysaccharides	1119:1152	The tree peony pod polysaccharides obtained	1119:1161	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	55	theme	galacturonic	1174:1185	arg1	polysaccharides					1224:1238	acidic polysaccharides	1217:1238	acidic polysaccharides	1217:1238	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	55	theme	galacturonic	1174:1185	arg1	galactose					1196:1204	galactose	1196:1204	galactose	1196:1204	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	3	56	dep	min	541:543	arg1	50°C					567:570	reaction temperature 50°C	546:570	30 min; reaction temperature 50°C	538:570	Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% ± 0.44%.					
30515387	7	57	theme	peony	1305:1309	arg1	polysaccharides					1315:1329	the tree peony pod polysaccharides	1296:1329	the tree peony pod polysaccharides	1296:1329	Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.					
30515387	6	58	theme	acidic	1217:1222	arg1	acid					1187:1190	galacturonic acid	1174:1190	galacturonic acid	1174:1190	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	58	theme	acidic	1217:1222	arg1	polysaccharides					1224:1238	acidic polysaccharides	1217:1238	acidic polysaccharides	1217:1238	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	58	theme	acidic	1217:1222	arg1	galactose					1196:1204	galactose	1196:1204	galactose	1196:1204	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	4	59	theme	tree	741:744	arg1	polysaccharides					756:770	the tree peony pod polysaccharides	737:770	the tree peony pod polysaccharides	737:770	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	2	60	theme	technological	325:337	arg1	factor					381:386	a single factor	372:386	a single factor to enhance the tree peony pod polysaccharide extraction yield	372:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	2	60	theme	technological	325:337	arg1	parameters					339:348	The technological parameters	321:348	The technological parameters	321:348	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	5	61	dep	1.44	1055:1058	arg1	0.21					1113:1116	0.21	1113:1116	0.21	1113:1116	The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21.					
30515387	0	62	theme	Tree	85:88	arg1	Pods					96:99	Tree Peony Pods	85:99	Tree Peony Pods	85:99	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	4	63	theme	polysaccharides	756:770	arg1	composition					722:732	the monosaccharide composition	703:732	the monosaccharide composition of the tree peony pod polysaccharides	703:770	Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method.					
30515387	1	64	theme	extraction	208:217	arg1	method					219:224	an ultrasonic-assisted extraction method	185:224	an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent	185:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	2	65	theme	single	374:379	arg1	factor					381:386	a single factor	372:386	a single factor to enhance the tree peony pod polysaccharide extraction yield	372:448	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	2	65	theme	single	374:379	arg1	parameters					339:348	The technological parameters	321:348	The technological parameters	321:348	The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield.					
30515387	1	66	theme	tree	257:260	arg1	pods					268:271	tree peony pods	257:271	tree peony pods using polyethylene glycol (PEG) as the solvent	257:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
30515387	0	67	theme	Efficient	10:18	arg1	Extraction					50:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Efficient PEG-Based Ultrasonic-Assisted Extraction	10:59	Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro.					
30515387	6	68	theme	peony	1128:1132	arg1	acid					1187:1190	galacturonic acid	1174:1190	galacturonic acid	1174:1190	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	6	68	theme	peony	1128:1132	arg1	polysaccharides					1138:1152	The tree peony pod polysaccharides	1119:1152	The tree peony pod polysaccharides obtained	1119:1161	The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides.					
30515387	1	69	theme	peony	262:266	arg1	pods					268:271	tree peony pods	257:271	tree peony pods using polyethylene glycol (PEG) as the solvent	257:318	We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent.					
29432964	2	0	theme	Fehling	523:529	arg1	solution					531:538	Fehling solution	523:538	Fehling solution	523:538	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	1	1	theme	polysaccharides	275:289	arg1	presence					253:260	the presence	249:260	the presence of bioactive polysaccharides	249:289	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	5	2	theme	lower	1014:1018	arg1	content					1020:1026	a lower content	1012:1026	a lower content of 3-O-Me-Gal	1012:1040	Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.					
29432964	3	3	theme	structural	545:554	arg1	assignments					556:566	The structural assignments	541:566	The structural assignments	541:566	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	3	4	theme	bidimensional	601:613	arg1	spectroscopy					619:630	NMR spectroscopy	615:630	NMR spectroscopy	615:630	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	3	5	theme	NMR	615:617	arg1	spectroscopy					619:630	NMR spectroscopy	615:630	NMR spectroscopy	615:630	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	1	6	contain	have	191:194	arg1	citrinopileatus					130:144	Pleurotus citrinopileatus	120:144	Pleurotus citrinopileatus	120:144	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	1	6	contain	have	191:194	arg2	properties					206:215	medicinal properties	196:215	medicinal properties	196:215	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	3	7	theme	mono-	591:595	arg1	spectroscopy					619:630	NMR spectroscopy	615:630	NMR spectroscopy	615:630	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	5	8	theme	3-O-Me-Gal	1031:1040	arg1	content					1020:1026	a lower content	1012:1026	a lower content of 3-O-Me-Gal	1012:1040	Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.					
29432964	5	9	theme	Similar	935:941	arg1	structures					943:952	Similar structures	935:952	Similar structures	935:952	Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.					
29432964	1	10	theme	medicinal	196:204	arg1	properties					206:215	medicinal properties	196:215	medicinal properties	196:215	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	4	11	theme	37.6 × 103 g/mol	882:897	arg1	masses					872:877	molar masses	866:877	molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol	866:918	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	2	12	theme	aqueous	415:421	arg1	extraction					423:432	successive aqueous extraction	404:432	successive aqueous extraction	404:432	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	0	13	theme	methylated	10:19	arg1	galactans					21:29	Partially methylated galactans	0:29	Partially methylated galactans	0:29	Partially methylated galactans containing different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus.					
29432964	1	14	theme	Pleurotus	120:128	arg1	citrinopileatus					130:144	Pleurotus citrinopileatus	120:144	Pleurotus citrinopileatus	120:144	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	2	15	theme	successive	404:413	arg1	extraction					423:432	successive aqueous extraction	404:432	successive aqueous extraction	404:432	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	4	16	theme	28.5 × 103 g/mol	903:918	arg1	masses					872:877	molar masses	866:877	molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol	866:918	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	4	17	theme	linear	729:734	arg1	α-galactopyranans					777:793	linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans	729:793	linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans	729:793	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	4	17	theme	linear	729:734	arg1	polysaccharides					691:705	The polysaccharides	687:705	The polysaccharides	687:705	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	4	18	dep	-linked	769:775	arg1	3-O-methylated					747:760	3-O-methylated	747:760	3-O-methylated	747:760	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	2	19	attach	isolated	350:357	arg2	galactans					335:343	two partially 3-O-methylated galactans	306:343	two partially 3-O-methylated galactans	306:343	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	2	19	attach	isolated	350:357	arg1	bodies					377:382	the fruiting bodies	364:382	the fruiting bodies of this fungus	364:397	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	3	20	theme	monosaccharide	633:646	arg1	composition					648:658	monosaccharide composition	633:658	monosaccharide composition	633:658	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	2	21	theme	3-O-methylated	320:333	arg1	galactans					335:343	two partially 3-O-methylated galactans	306:343	two partially 3-O-methylated galactans	306:343	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	2	22	theme	material	509:516	arg1	extraction					423:432	successive aqueous extraction	404:432	successive aqueous extraction	404:432	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	2	22	theme	material	509:516	arg1	precipitation					484:496	precipitation	484:496	precipitation of soluble material with Fehling solution	484:538	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	5	23	theme	Pleurotus	984:992	arg1	spp.					994:997	other Pleurotus spp.	978:997	other Pleurotus spp.	978:997	Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.					
29432964	0	24	from	citrinopileatus	103:117	arg1	proportions					52:62	different proportions	42:62	different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus	42:117	Partially methylated galactans containing different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus.					
29432964	4	25	theme	molar	866:870	arg1	masses					872:877	molar masses	866:877	molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol	866:918	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	2	26	theme	soluble	501:507	arg1	material					509:516	soluble material	501:516	soluble material with Fehling solution	501:538	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	0	27	theme	different	42:50	arg1	proportions					52:62	different proportions	42:62	different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus	42:117	Partially methylated galactans containing different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus.					
29432964	0	28	theme	3-O-methyl-galactose	67:86	arg1	proportions					52:62	different proportions	42:62	different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus	42:117	Partially methylated galactans containing different proportions of 3-O-methyl-galactose from Pleurotus citrinopileatus.					
29432964	5	29	theme	other	978:982	arg1	spp.					994:997	other Pleurotus spp.	978:997	other Pleurotus spp.	978:997	Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.					
29432964	1	30	theme	mushroom	181:188	arg1	"					189:189	"golden oyster mushroom"	166:189	"golden oyster mushroom"	166:189	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	2	31	theme	fruiting	368:375	arg1	bodies					377:382	the fruiting bodies	364:382	the fruiting bodies of this fungus	364:397	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	4	32	theme	molar	847:851	arg1	ratios					853:858	1:1 molar ratios	843:858	1:1 molar ratios	843:858	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	4	33	theme	1:1	843:845	arg1	ratios					853:858	1:1 molar ratios	843:858	1:1 molar ratios	843:858	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	1	34	theme	golden	167:172	arg1	"					189:189	"golden oyster mushroom"	166:189	"golden oyster mushroom"	166:189	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	4	35	dep	linear	729:734	arg1	-linked					769:775	-linked	769:775	-linked	769:775	The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 103 g/mol and 28.5 × 103 g/mol, respectively.					
29432964	1	36	theme	oyster	174:179	arg1	"					189:189	"golden oyster mushroom"	166:189	"golden oyster mushroom"	166:189	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
29432964	3	37	theme	methylation	665:675	arg1	analyses					677:684	methylation analyses	665:684	methylation analyses	665:684	The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.					
29432964	2	38	with	material	509:516	arg1	solution					531:538	Fehling solution	523:538	Fehling solution	523:538	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	2	39	theme	fungus	392:397	arg1	bodies					377:382	the fruiting bodies	364:382	the fruiting bodies of this fungus	364:397	In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution.					
29432964	1	40	theme	bioactive	265:273	arg1	polysaccharides					275:289	bioactive polysaccharides	265:289	bioactive polysaccharides	265:289	Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides.					
30119885	2	0	theme	tumor-targeting	353:367	arg1	mAbs					369:372	some tumor-targeting mAbs	348:372	some tumor-targeting mAbs	348:372	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	3	1	theme	Chinese	775:781	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	1	theme	Chinese	775:781	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	1	theme	Chinese	775:781	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	6	2	theme	Fc-engineered	1621:1633	arg1	mAbs					1635:1638	Fc-engineered mAbs	1621:1638	Fc-engineered mAbs	1621:1638	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	5	3	with	mAbs	1435:1438	arg1	functions					1472:1480	characteristic Fc-mediated functions	1445:1480	characteristic Fc-mediated functions	1445:1480	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	5	4	theme	Fc-mediated	1460:1470	arg1	functions					1472:1480	characteristic Fc-mediated functions	1445:1480	characteristic Fc-mediated functions	1445:1480	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	2	5	theme	effector	274:281	arg1	functions					283:291	Fc-mediated effector functions	262:291	Fc-mediated effector functions	262:291	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	3	6	theme	CHO	798:800	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	6	theme	CHO	798:800	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	6	theme	CHO	798:800	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	7	7	theme	Transgenic	1748:1757	arg1	silkworms					1759:1767	Transgenic silkworms	1748:1767	Transgenic silkworms	1748:1767	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	7	7	theme	Transgenic	1748:1757	arg1	system					1798:1803	a promising system	1786:1803	a promising system for the production of Fc-engineered mAbs	1786:1844	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	4	8	theme	Fc-mediated	1145:1155	arg1	function					1157:1164	the Fc-mediated function	1141:1164	the Fc-mediated function of mAbs	1141:1172	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	3	9	theme	ovary	791:795	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	9	theme	ovary	791:795	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	9	theme	ovary	791:795	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	2	10	theme	pivotal	300:306	arg1	role					308:311	a pivotal role	298:311	a pivotal role	298:311	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	4	11	theme	acid	961:964	arg1	substitutions					966:978	amino acid substitutions	955:978	amino acid substitutions using transgenic silkworms	955:1005	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	0	12	theme	Bombyx	130:135	arg1	silkworms					119:127	transgenic silkworms	108:127	transgenic silkworms (Bombyx mori)	108:141	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	0	12	theme	Bombyx	130:135	arg1	mori					137:140	Bombyx mori	130:140	Bombyx mori	130:140	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	4	13	theme	anti-CD20	935:943	arg1	mAbs					945:948	anti-CD20 mAbs	935:948	anti-CD20 mAbs	935:948	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	5	14	theme	silkworm-derived	1332:1347	arg1	mAbs					1349:1352	silkworm-derived mAbs	1332:1352	silkworm-derived mAbs	1332:1352	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	15	theme	stronger	670:677	arg1	activity					684:691	stronger ADCC activity	670:691	stronger ADCC activity	670:691	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	6	16	theme	Fc-mediated	1654:1664	arg1	functions					1677:1685	suitable Fc-mediated biological functions	1645:1685	suitable Fc-mediated biological functions	1645:1685	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	3	17	theme	non-reducing	873:884	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	17	theme	non-reducing	873:884	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	5	18	link	silkworm-derived	1332:1347	arg1	mAbs					1349:1352	silkworm-derived mAbs	1332:1352	silkworm-derived mAbs	1332:1352	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	19	theme	unique	822:827	arg1	structure					838:846	their unique N-glycan structure	816:846	their unique N-glycan structure	816:846	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	4	20	theme	biological	1026:1035	arg1	activities					1037:1046	their biological activities	1020:1046	their biological activities	1020:1046	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	5	21	theme	Fc-mediated	1295:1305	arg1	activities					1318:1327	the Fc-mediated biological activities	1291:1327	the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs	1291:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	22	theme	core-fucose	857:867	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	22	theme	core-fucose	857:867	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	22	theme	core-fucose	857:867	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	1	23	theme	monoclonal	156:165	arg1	mAbs					179:182	mAbs	179:182	mAbs	179:182	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	1	23	theme	monoclonal	156:165	arg1	antibodies					167:176	Recombinant monoclonal antibodies	144:176	Recombinant monoclonal antibodies (mAbs)	144:183	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	0	24	from	Effects	0:6	arg1	activity					54:61	the biological activity	39:61	the biological activity of anti-CD20 monoclonal antibody	39:94	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	0	25	theme	antibody	87:94	arg1	activity					54:61	the biological activity	39:61	the biological activity of anti-CD20 monoclonal antibody	39:94	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	3	26	dep	cells	803:807	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	26	dep	cells	803:807	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	26	dep	cells	803:807	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	6	27	theme	actions	1739:1745	arg1	mechanism					1720:1728	the pharmacological mechanism	1700:1728	the pharmacological mechanism of their actions	1700:1745	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	7	28	theme	promising	1788:1796	arg1	silkworms					1759:1767	Transgenic silkworms	1748:1767	Transgenic silkworms	1748:1767	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	7	28	theme	promising	1788:1796	arg1	system					1798:1803	a promising system	1786:1803	a promising system for the production of Fc-engineered mAbs	1786:1844	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	2	29	theme	antibody-dependent	541:558	arg1	ADCC					583:586	ADCC	583:586	ADCC	583:586	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	2	29	theme	antibody-dependent	541:558	arg1	cytotoxicity					569:580	antibody-dependent cellular cytotoxicity	541:580	antibody-dependent cellular cytotoxicity (ADCC)	541:587	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	2	30	from	region	477:482	arg1	substitutions					444:456	glyco-engineering or amino acid substitutions	412:456	substitutions	444:456	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	5	31	theme	mAbs	1384:1387	arg1	case					1364:1367	the case	1360:1367	the case of CHO-derived mAbs	1360:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	2	32	theme	glyco-engineering	412:428	arg1	substitutions					444:456	glyco-engineering or amino acid substitutions	412:456	substitutions	444:456	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	7	33	theme	Fc-engineered	1827:1839	arg1	mAbs					1841:1844	Fc-engineered mAbs	1827:1844	Fc-engineered mAbs	1827:1844	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	2	34	theme	Fc	474:475	arg1	region					477:482	the antibody Fc region	461:482	the antibody Fc region	461:482	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	6	35	theme	amino	1502:1506	arg1	substitutions					1513:1525	amino acid substitutions	1502:1525	amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1502:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	6	36	theme	pharmacological	1704:1718	arg1	mechanism					1720:1728	the pharmacological mechanism	1700:1728	the pharmacological mechanism of their actions	1700:1745	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	4	37	theme	combination	1076:1086	arg1	effect					1062:1067	the effect	1058:1067	the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs	1058:1172	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	0	38	theme	amino	11:15	arg1	substitutions					22:34	amino acid substitutions	11:34	amino acid substitutions	11:34	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	3	39	theme	lower	697:701	arg1	activity					743:750	lower complement-dependent cytotoxicity (CDC) activity	697:750	lower complement-dependent cytotoxicity (CDC) activity	697:750	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	7	40	theme	mAbs	1841:1844	arg1	production					1813:1822	the production	1809:1822	the production of Fc-engineered mAbs	1809:1844	Transgenic silkworms were shown to be a promising system for the production of Fc-engineered mAbs.					
30119885	2	41	theme	amino	433:437	arg1	substitutions					444:456	glyco-engineering or amino acid substitutions	412:456	substitutions	444:456	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	0	42	theme	substitutions	22:34	arg1	Effects					0:6	Effects	0:6	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody	0:94	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	5	43	theme	amino	1190:1194	arg1	substitutions					1201:1213	amino acid substitutions	1190:1213	amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1190:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	6	44	from	region	1537:1542	arg1	substitutions					1513:1525	amino acid substitutions	1502:1525	amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1502:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	6	44	from	region	1537:1542	arg1	combination					1487:1497	The combination	1483:1497	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1483:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	5	45	theme	mAbs	1435:1438	arg1	generation					1407:1416	the generation	1403:1416	the generation of Fc-engineered mAbs with characteristic Fc-mediated functions	1403:1480	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	5	46	theme	substitutions	1201:1213	arg1	types					1181:1185	Three types	1175:1185	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1175:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	6	47	from	glyco-engineering	1548:1564	arg1	substitutions					1513:1525	amino acid substitutions	1502:1525	amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1502:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	6	47	from	glyco-engineering	1548:1564	arg1	combination					1487:1497	The combination	1483:1497	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1483:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	4	48	theme	substitutions	1124:1136	arg1	combination					1076:1086	the combination	1072:1086	the combination of glyco-engineering and amino acid substitutions	1072:1136	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	5	49	theme	characteristic	1445:1458	arg1	functions					1472:1480	characteristic Fc-mediated functions	1445:1480	characteristic Fc-mediated functions	1445:1480	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	1	50	theme	various	203:209	arg1	applications					223:234	various therapeutic applications	203:234	various therapeutic applications including cancer therapy	203:259	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	1	50	theme	various	203:209	arg1	therapy					253:259	cancer therapy	246:259	cancer therapy	246:259	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	3	51	theme	cytotoxicity	724:735	arg1	activity					743:750	lower complement-dependent cytotoxicity (CDC) activity	697:750	lower complement-dependent cytotoxicity (CDC) activity	697:750	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	2	52	with	technologies	394:405	arg1	substitutions					444:456	glyco-engineering or amino acid substitutions	412:456	substitutions	444:456	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	4	53	theme	glyco-engineering	1091:1107	arg1	combination					1076:1086	the combination	1072:1086	the combination of glyco-engineering and amino acid substitutions	1072:1136	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	2	54	theme	mAbs	369:372	arg1	activities					334:343	the tumor-killing activities	316:343	the tumor-killing activities of some tumor-targeting mAbs	316:372	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	1	55	theme	cancer	246:251	arg1	therapy					253:259	cancer therapy	246:259	cancer therapy	246:259	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	4	56	theme	amino	1113:1117	arg1	substitutions					1124:1136	amino acid substitutions	1113:1136	amino acid substitutions	1113:1136	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	2	57	used	used	494:497	arg2	technologies					394:405	Fc-engineering technologies	379:405	Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region	379:482	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	6	58	theme	substitutions	1513:1525	arg1	combination					1487:1497	The combination	1483:1497	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1483:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	5	59	from	activities	1318:1327	arg1	case					1364:1367	the case	1360:1367	the case of CHO-derived mAbs	1360:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	60	theme	hamster	783:789	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	60	theme	hamster	783:789	arg1	lack					849:852	lack	849:852	lack of core-fucose	849:867	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	60	theme	hamster	783:789	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	5	61	from	region	1225:1230	arg1	substitutions					1201:1213	amino acid substitutions	1190:1213	amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1190:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	5	61	from	region	1225:1230	arg1	types					1181:1185	Three types	1175:1185	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1175:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	4	62	theme	mAbs	1169:1172	arg1	function					1157:1164	the Fc-mediated function	1141:1164	the Fc-mediated function of mAbs	1141:1172	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	6	63	from	combination	1487:1497	arg1	glyco-engineering					1548:1564	glyco-engineering	1548:1564	glyco-engineering	1548:1564	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	6	63	from	combination	1487:1497	arg1	region					1537:1542	the Fc region	1530:1542	the Fc region	1530:1542	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	6	64	theme	transgenic	1572:1581	arg1	silkworm					1583:1590	transgenic silkworm	1572:1590	transgenic silkworm	1572:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	4	65	theme	amino	955:959	arg1	substitutions					966:978	amino acid substitutions	955:978	amino acid substitutions using transgenic silkworms	955:1005	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	1	66	used	used	195:198	arg2	antibodies					167:176	Recombinant monoclonal antibodies	144:176	Recombinant monoclonal antibodies (mAbs)	144:183	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	1	66	used	used	195:198	arg2	mAbs					179:182	mAbs	179:182	mAbs	179:182	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	2	67	theme	Fc-mediated	262:272	arg1	functions					283:291	Fc-mediated effector functions	262:291	Fc-mediated effector functions	262:291	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	6	68	theme	biological	1666:1675	arg1	functions					1677:1685	suitable Fc-mediated biological functions	1645:1685	suitable Fc-mediated biological functions	1645:1685	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	5	69	theme	mAbs	1349:1352	arg1	activities					1318:1327	the Fc-mediated biological activities	1291:1327	the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs	1291:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	70	theme	transgenic	642:651	arg1	silkworms					653:661	transgenic silkworms	642:661	transgenic silkworms	642:661	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	6	71	theme	suitable	1645:1652	arg1	functions					1677:1685	suitable Fc-mediated biological functions	1645:1685	suitable Fc-mediated biological functions	1645:1685	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	3	72	theme	terminal	886:893	arg1	galactose					895:903	non-reducing terminal galactose	873:903	non-reducing terminal galactose	873:903	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	72	theme	terminal	886:893	arg1	cells					803:807	Chinese hamster ovary (CHO) cells	775:807	Chinese hamster ovary (CHO) cells	775:807	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	2	73	theme	cytotoxic	510:518	arg1	activities					520:529	cytotoxic activities	510:529	cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC)	510:587	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	2	73	theme	cytotoxic	510:518	arg1	cytotoxicity					569:580	antibody-dependent cellular cytotoxicity	541:580	antibody-dependent cellular cytotoxicity (ADCC)	541:587	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	5	74	dep	region	1225:1230	arg1	L234A/L235A					1252:1262	L234A/L235A	1252:1262	L234A/L235A	1252:1262	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	5	74	dep	region	1225:1230	arg1	K326W/E333S					1269:1279	K326W/E333S	1269:1279	K326W/E333S	1269:1279	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	5	74	dep	region	1225:1230	arg1	G236A/S239D/I332E					1233:1249	G236A/S239D/I332E	1233:1249	G236A/S239D/I332E	1233:1249	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	3	75	theme	N-glycan	829:836	arg1	structure					838:846	their unique N-glycan structure	816:846	their unique N-glycan structure	816:846	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	0	76	theme	monoclonal	76:85	arg1	antibody					87:94	anti-CD20 monoclonal antibody	66:94	anti-CD20 monoclonal antibody	66:94	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	1	77	theme	Recombinant	144:154	arg1	mAbs					179:182	mAbs	179:182	mAbs	179:182	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	1	77	theme	Recombinant	144:154	arg1	antibodies					167:176	Recombinant monoclonal antibodies	144:176	Recombinant monoclonal antibodies (mAbs)	144:183	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	5	78	theme	biological	1307:1316	arg1	activities					1318:1327	the Fc-mediated biological activities	1291:1327	the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs	1291:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	2	79	theme	cellular	560:567	arg1	ADCC					583:586	ADCC	583:586	ADCC	583:586	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	2	79	theme	cellular	560:567	arg1	cytotoxicity					569:580	antibody-dependent cellular cytotoxicity	541:580	antibody-dependent cellular cytotoxicity (ADCC)	541:587	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	5	80	theme	CHO-derived	1372:1382	arg1	mAbs					1384:1387	CHO-derived mAbs	1372:1387	CHO-derived mAbs	1372:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	0	81	theme	transgenic	108:117	arg1	silkworms					119:127	transgenic silkworms	108:127	transgenic silkworms (Bombyx mori)	108:141	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	0	81	theme	transgenic	108:117	arg1	mori					137:140	Bombyx mori	130:140	Bombyx mori	130:140	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	4	82	theme	transgenic	986:995	arg1	silkworms					997:1005	transgenic silkworms	986:1005	transgenic silkworms	986:1005	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	5	83	link	CHO-derived	1372:1382	arg1	mAbs					1384:1387	CHO-derived mAbs	1372:1387	CHO-derived mAbs	1372:1387	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	6	84	theme	acid	1508:1511	arg1	substitutions					1513:1525	amino acid substitutions	1502:1525	amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm	1502:1590	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
30119885	2	85	theme	antibody	465:472	arg1	region					477:482	the antibody Fc region	461:482	the antibody Fc region	461:482	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	2	86	theme	Fc-engineering	379:392	arg1	technologies					394:405	Fc-engineering technologies	379:405	Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region	379:482	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	0	87	theme	acid	17:20	arg1	substitutions					22:34	amino acid substitutions	11:34	amino acid substitutions	11:34	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	5	88	theme	Fc	1222:1223	arg1	region					1225:1230	the Fc region	1218:1230	the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1218:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	2	89	theme	acid	439:442	arg1	substitutions					444:456	glyco-engineering or amino acid substitutions	412:456	substitutions	444:456	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	5	90	from	types	1181:1185	arg1	region					1225:1230	the Fc region	1218:1230	the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1218:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	4	91	from	effect	1062:1067	arg1	function					1157:1164	the Fc-mediated function	1141:1164	the Fc-mediated function of mAbs	1141:1172	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	3	92	theme	ADCC	679:682	arg1	activity					684:691	stronger ADCC activity	670:691	stronger ADCC activity	670:691	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	5	93	theme	Fc-engineered	1421:1433	arg1	mAbs					1435:1438	Fc-engineered mAbs	1421:1438	Fc-engineered mAbs with characteristic Fc-mediated functions	1421:1480	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	0	94	theme	biological	43:52	arg1	activity					54:61	the biological activity	39:61	the biological activity of anti-CD20 monoclonal antibody	39:94	Effects of amino acid substitutions on the biological activity of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).					
30119885	5	95	theme	acid	1196:1199	arg1	substitutions					1201:1213	amino acid substitutions	1190:1213	amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S)	1190:1280	Three types of amino acid substitutions at the Fc region (G236A/S239D/I332E, L234A/L235A, and K326W/E333S) modified the Fc-mediated biological activities of silkworm-derived mAbs as in the case of CHO-derived mAbs, resulting in the generation of Fc-engineered mAbs with characteristic Fc-mediated functions.					
30119885	4	96	theme	acid	1119:1122	arg1	substitutions					1124:1136	amino acid substitutions	1113:1136	amino acid substitutions	1113:1136	In this study, we generated anti-CD20 mAbs with amino acid substitutions using transgenic silkworms and analyzed their biological activities to assess the effect of the combination of glyco-engineering and amino acid substitutions on the Fc-mediated function of mAbs.					
30119885	3	97	theme	complement-dependent	703:722	arg1	cytotoxicity					724:735	complement-dependent cytotoxicity	703:735	lower complement-dependent cytotoxicity (CDC) activity	697:750	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	3	97	theme	complement-dependent	703:722	arg1	CDC					738:740	CDC	738:740	CDC	738:740	We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose).					
30119885	2	98	theme	tumor-killing	320:332	arg1	activities					334:343	the tumor-killing activities	316:343	the tumor-killing activities of some tumor-targeting mAbs	316:372	Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC).					
30119885	1	99	theme	therapeutic	211:221	arg1	applications					223:234	various therapeutic applications	203:234	various therapeutic applications including cancer therapy	203:259	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	1	99	theme	therapeutic	211:221	arg1	therapy					253:259	cancer therapy	246:259	cancer therapy	246:259	Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy.					
30119885	6	100	theme	Fc	1534:1535	arg1	region					1537:1542	the Fc region	1530:1542	the Fc region	1530:1542	The combination of amino acid substitutions at the Fc region and glyco-engineering using transgenic silkworm made it possible to generate Fc-engineered mAbs with suitable Fc-mediated biological functions depending on the pharmacological mechanism of their actions.					
31176856	0	0	theme	natural	88:94	arg1	hydrogels					117:125	natural polysaccharide-based hydrogels	88:125	natural polysaccharide-based hydrogels	88:125	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	2	1	theme	enzyme	530:535	arg1	concentration					537:549	39.88 mg mL-1 initial enzyme concentration	508:549	39.88 mg mL-1 initial enzyme concentration	508:549	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	1	2	theme	composite	179:187	arg1	FFCCRD					208:213	FFCCRD	208:213	FFCCRD	208:213	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	2	theme	composite	179:187	arg1	design					200:205	A full-factorial central composite rotational design	154:205	A full-factorial central composite rotational design (FFCCRD)	154:214	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	2	3	theme	initial	522:528	arg1	concentration					537:549	39.88 mg mL-1 initial enzyme concentration	508:549	39.88 mg mL-1 initial enzyme concentration	508:549	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	3	4	theme	UHT	741:743	arg1	milk					745:748	UHT milk	741:748	UHT milk	741:748	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	1	5	theme	rotational	189:198	arg1	FFCCRD					208:213	FFCCRD	208:213	FFCCRD	208:213	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	5	theme	rotational	189:198	arg1	design					200:205	A full-factorial central composite rotational design	154:205	A full-factorial central composite rotational design (FFCCRD)	154:214	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	6	theme	Arabic	274:279	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	7	from	hydrolysis	325:334	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	8	theme	gum-based	281:289	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	0	9	theme	polysaccharide-based	96:115	arg1	hydrogels					117:125	natural polysaccharide-based hydrogels	88:125	natural polysaccharide-based hydrogels	88:125	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	0	10	from	immobilization	59:72	arg1	hydrogels					117:125	natural polysaccharide-based hydrogels	88:125	natural polysaccharide-based hydrogels	88:125	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	0	10	from	immobilization	59:72	arg1	hydrolysis					131:140	hydrolysis	131:140	hydrolysis of lactose	131:151	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	3	11	theme	released	599:606	arg1	fraction					608:615	released fraction	599:615	released fraction from the Arabic gum-based hydrogel	599:650	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	6	12	theme	lactase	1074:1080	arg1	immobilization					1056:1069	the immobilization	1052:1069	the immobilization of lactase and hydrolysis of lactose	1052:1106	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	3	13	theme	enzyme	579:584	arg1	0.322 U mg-1					657:668	0.322 U mg-1	657:668	0.322 U mg-1	657:668	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	3	13	theme	enzyme	579:584	arg1	activity					586:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	2	14	theme	immobilized	426:436	arg1	activity					445:452	high immobilized enzyme activity	421:452	high immobilized enzyme activity	421:452	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	4	15	theme	chitosan-based	815:828	arg1	hydrogel					830:837	a chitosan-based hydrogel	813:837	a chitosan-based hydrogel	813:837	These values were 0.289 U mg-1 and 0.136, respectively, using a chitosan-based hydrogel.					
31176856	2	16	theme	high	421:424	arg1	activity					445:452	high immobilized enzyme activity	421:452	high immobilized enzyme activity	421:452	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	0	17	theme	central	15:21	arg1	design					44:49	Full-factorial central composite rotational design	0:49	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.	0:152	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	3	18	theme	immobilized	567:577	arg1	0.322 U mg-1					657:668	0.322 U mg-1	657:668	0.322 U mg-1	657:668	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	3	18	theme	immobilized	567:577	arg1	activity					586:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	0	19	theme	Full-factorial	0:13	arg1	design					44:49	Full-factorial central composite rotational design	0:49	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.	0:152	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	1	20	theme	chitosan-based	295:308	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	21	from	hydrogels	310:318	arg1	immobilization					245:258	the immobilization	241:258	the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels	241:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	21	from	hydrogels	310:318	arg1	hydrolysis					325:334	hydrolysis	325:334	hydrolysis of lactose	325:345	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	3	22	theme	Arabic	626:631	arg1	hydrogel					643:650	the Arabic gum-based hydrogel	622:650	the Arabic gum-based hydrogel	622:650	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	5	23	theme	standard	940:947	arg1	lactose					949:955	standard lactose	940:955	standard lactose	940:955	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	0	24	theme	rotational	33:42	arg1	design					44:49	Full-factorial central composite rotational design	0:49	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.	0:152	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	6	25	theme	best	1032:1035	arg1	conditions					1037:1046	The best conditions	1028:1046	The best conditions for the immobilization of lactase and hydrolysis of lactose	1028:1106	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	0	26	theme	lactose	145:151	arg1	hydrogels					117:125	natural polysaccharide-based hydrogels	88:125	natural polysaccharide-based hydrogels	88:125	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	0	26	theme	lactose	145:151	arg1	hydrolysis					131:140	hydrolysis	131:140	hydrolysis of lactose	131:151	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	3	27	from	hydrogel	643:650	arg1	0.322 U mg-1					657:668	0.322 U mg-1	657:668	0.322 U mg-1	657:668	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	3	27	from	hydrogel	643:650	arg1	activity					586:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity	563:593	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	3	27	from	hydrogel	643:650	arg1	fraction					608:615	released fraction	599:615	released fraction from the Arabic gum-based hydrogel	599:650	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	2	28	theme	low	458:460	arg1	fraction					471:478	low released fraction	458:478	low released fraction	458:478	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	0	29	theme	composite	23:31	arg1	design					44:49	Full-factorial central composite rotational design	0:49	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.	0:152	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	5	30	theme	lactose	949:955	arg1	hydrolysis					926:935	the hydrolysis	922:935	the hydrolysis of standard lactose	922:955	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	2	31	theme	39.88 mg mL-1	508:520	arg1	concentration					537:549	39.88 mg mL-1 initial enzyme concentration	508:549	39.88 mg mL-1 initial enzyme concentration	508:549	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	5	32	theme	released	876:883	arg1	fraction					885:892	released fraction	876:892	released fraction from these hydrogels during the hydrolysis of standard lactose	876:955	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	5	33	from	hydrogels	905:913	arg1	0.246 U mg-1					962:973	0.246 U mg-1	962:973	0.246 U mg-1	962:973	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	5	33	from	hydrogels	905:913	arg1	activity					863:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	5	33	from	hydrogels	905:913	arg1	fraction					885:892	released fraction	876:892	released fraction from these hydrogels during the hydrolysis of standard lactose	876:955	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	2	34	theme	enzyme	438:443	arg1	activity					445:452	high immobilized enzyme activity	421:452	high immobilized enzyme activity	421:452	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	5	35	theme	immobilized	844:854	arg1	0.246 U mg-1					962:973	0.246 U mg-1	962:973	0.246 U mg-1	962:973	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	5	35	theme	immobilized	844:854	arg1	activity					863:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	6	36	theme	lactose	1100:1106	arg1	hydrolysis					1086:1095	hydrolysis	1086:1095	hydrolysis of lactose	1086:1106	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	6	36	theme	lactose	1100:1106	arg1	lactase					1074:1080	lactase	1074:1080	lactase	1074:1080	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	3	37	theme	gum-based	633:641	arg1	hydrogel					643:650	the Arabic gum-based hydrogel	622:650	the Arabic gum-based hydrogel	622:650	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	6	38	theme	experimental	1167:1178	arg1	results					1180:1186	experimental results	1167:1186	experimental results	1167:1186	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	5	39	theme	enzyme	856:861	arg1	0.246 U mg-1					962:973	0.246 U mg-1	962:973	0.246 U mg-1	962:973	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	5	39	theme	enzyme	856:861	arg1	activity					863:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity	840:870	The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg-1 and 0.407, and 0.211 U mg-1 and 0.245, respectively.					
31176856	1	40	theme	lactose	339:345	arg1	immobilization					245:258	the immobilization	241:258	the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels	241:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	40	theme	lactose	339:345	arg1	hydrolysis					325:334	hydrolysis	325:334	hydrolysis of lactose	325:345	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	41	from	lactase	263:269	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	2	42	theme	immobilization	360:373	arg1	capacities					375:384	The optimal immobilization capacities	348:384	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction	348:478	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	2	43	theme	optimal	352:358	arg1	capacities					375:384	The optimal immobilization capacities	348:384	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction	348:478	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	1	44	from	immobilization	245:258	arg1	hydrogels					310:318	Arabic gum-based and chitosan-based hydrogels	274:318	Arabic gum-based and chitosan-based hydrogels	274:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	2	45	theme	hydrogels	394:402	arg1	capacities					375:384	The optimal immobilization capacities	348:384	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction	348:478	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	6	46	theme	hydrolysis	1086:1095	arg1	immobilization					1056:1069	the immobilization	1052:1069	the immobilization of lactase and hydrolysis of lactose	1052:1106	The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.					
31176856	0	47	theme	lactase	77:83	arg1	immobilization					59:72	the immobilization	55:72	the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose	55:151	Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose.					
31176856	3	48	theme	lactose	720:726	arg1	hydrolysis					706:715	the hydrolysis	702:715	the hydrolysis of lactose contained in UHT milk	702:748	The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk.					
31176856	1	49	theme	full-factorial	156:169	arg1	FFCCRD					208:213	FFCCRD	208:213	FFCCRD	208:213	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	49	theme	full-factorial	156:169	arg1	design					200:205	A full-factorial central composite rotational design	154:205	A full-factorial central composite rotational design (FFCCRD)	154:214	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	2	50	theme	released	462:469	arg1	fraction					471:478	low released fraction	458:478	low released fraction	458:478	The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL-1 initial enzyme concentration and pH 6.5.					
31176856	1	51	theme	central	171:177	arg1	FFCCRD					208:213	FFCCRD	208:213	FFCCRD	208:213	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	51	theme	central	171:177	arg1	design					200:205	A full-factorial central composite rotational design	154:205	A full-factorial central composite rotational design (FFCCRD)	154:214	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	52	theme	lactase	263:269	arg1	immobilization					245:258	the immobilization	241:258	the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels	241:318	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
31176856	1	52	theme	lactase	263:269	arg1	hydrolysis					325:334	hydrolysis	325:334	hydrolysis of lactose	325:345	A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose.					
29624401	3	0	theme	reversible	684:693	arg1	hydration					695:703	their reversible hydration	678:703	their reversible hydration (thermogravimetric analysis)	678:732	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	0	theme	reversible	684:693	arg1	analysis					724:731	thermogravimetric analysis	706:731	thermogravimetric analysis	706:731	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	4	1	dep	backbones	1031:1039	arg1	intercalating					998:1010	intercalating	998:1010	intercalating	998:1010	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	3	2	theme	thermogravimetric	706:722	arg1	hydration					695:703	their reversible hydration	678:703	their reversible hydration (thermogravimetric analysis)	678:732	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	2	theme	thermogravimetric	706:722	arg1	analysis					724:731	thermogravimetric analysis	706:731	thermogravimetric analysis	706:731	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	3	theme	scanning	808:815	arg1	gun					852:854	scanning electron microscopy-field emission gun	808:854	scanning electron microscopy-field emission gun	808:854	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	3	theme	scanning	808:815	arg1	dimensions					785:794	dimensions	785:794	dimensions	785:794	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	0	4	theme	Specific	84:91	arg1	Properties					93:102	Specific Properties	84:102	Specific Properties of the Platelets	84:119	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	1	5	theme	soluble	331:337	arg1	molecules					339:347	poorly soluble molecules	324:347	poorly soluble molecules	324:347	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	6	6	theme	incomplete	1311:1320	arg1	terraces					1330:1337	terraces	1330:1337	terraces	1330:1337	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	6	6	theme	incomplete	1311:1320	arg1	layers					1322:1327	incomplete layers	1311:1327	incomplete layers (terraces)	1311:1338	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	8	7	theme	potential	1483:1491	arg1	relation					1493:1500	the potential relation	1479:1500	the potential relation between the platelet structure and applications for mucoadhesive devices	1479:1573	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	1	8	theme	guest	242:246	arg1	molecules					248:256	guest molecules	242:256	guest molecules in their internal cavity	242:281	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	3	9	theme	thermal	858:864	arg1	properties					866:875	thermal properties	858:875	thermal properties	858:875	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	2	10	with	CDs	389:391	arg1	chains					427:432	alkyl grafted polysaccharide chains	398:432	alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	398:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	4	11	theme	crystalline	949:959	arg1	platelets					961:969	The crystalline platelets	945:969	The crystalline platelets	945:969	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	5	12	theme	columnar-type	1119:1131	arg1	crystals					1133:1140	columnar-type crystals	1119:1140	columnar-type crystals	1119:1140	The monoclinic symmetry of columnar-type crystals suggests a head-to-tail arrangement of the CDs.					
29624401	4	13	theme	alkyl	1078:1082	arg1	chains					1084:1089	the grafted alkyl chains	1066:1089	the grafted alkyl chains	1066:1089	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	1	14	theme	oligosaccharides	158:173	arg1	Cyclodextrins					122:134	Cyclodextrins	122:134	Cyclodextrins (CDs)	122:140	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	1	14	theme	oligosaccharides	158:173	arg1	family					148:153	a family	146:153	a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules	146:347	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	0	15	theme	Platelets	111:119	arg1	Structure					70:78	Crystal Structure	62:78	Crystal Structure	62:78	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	0	15	theme	Platelets	111:119	arg1	Properties					93:102	Specific Properties	84:102	Specific Properties of the Platelets	84:119	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	5	16	theme	crystals	1133:1140	arg1	symmetry					1107:1114	The monoclinic symmetry	1092:1114	The monoclinic symmetry of columnar-type crystals	1092:1140	The monoclinic symmetry of columnar-type crystals suggests a head-to-tail arrangement of the CDs.					
29624401	6	17	contain	have	1204:1207	arg2	shape					1236:1240	a preferentially hexagonal shape	1209:1240	a preferentially hexagonal shape	1209:1240	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	6	17	contain	have	1204:1207	arg1	platelets					1194:1202	The platelets	1190:1202	The platelets	1190:1202	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	2	18	theme	inclusions	373:382	arg1	types					364:368	new types	360:368	new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	360:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	4	19	theme	CD	1045:1046	arg1	complexes					1048:1056	CD complexes	1045:1056	CD complexes hosting the grafted alkyl chains	1045:1089	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	4	20	theme	layered	979:985	arg1	structures					987:996	layered structures	979:996	layered structures intercalating the polysaccharide backbones	979:1039	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	8	21	dep	structure	1523:1531	arg1	the					1510:1512	the	1510:1512	the	1510:1512	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	8	22	theme	mucoadhesive	1554:1565	arg1	devices					1567:1573	mucoadhesive devices	1554:1573	mucoadhesive devices	1554:1573	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	3	23	theme	crystalline	735:745	arg1	structure					747:755	crystalline structure	735:755	crystalline structure (powder X-ray diffraction)	735:782	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	23	theme	crystalline	735:745	arg1	diffraction					771:781	powder X-ray diffraction	758:781	powder X-ray diffraction	758:781	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	2	24	dep	chains	427:432	arg1	amylopectin					464:474	amylopectin	464:474	amylopectin	464:474	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	24	dep	chains	427:432	arg1	sulfate					489:495	chondroitin sulfate	477:495	chondroitin sulfate	477:495	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	24	dep	chains	427:432	arg1	dextran					455:461	dextran	455:461	dextran	455:461	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	24	dep	chains	427:432	arg1	chitosan					445:452	chitosan	445:452	chitosan	445:452	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	24	dep	chains	427:432	arg1	pullulan					435:442	pullulan	435:442	pullulan	435:442	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	3	25	theme	scanning	922:929	arg1	calorimetry					931:941	micro-differential scanning calorimetry	903:941	micro-differential scanning calorimetry	903:941	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	1	26	theme	toroid	182:187	arg1	shape					189:193	a toroid shape	180:193	a toroid shape	180:193	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	2	27	theme	polysaccharide	412:425	arg1	chains					427:432	alkyl grafted polysaccharide chains	398:432	alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	398:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	1	28	theme	internal	267:274	arg1	cavity					276:281	their internal cavity	261:281	their internal cavity	261:281	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	1	29	from	molecules	248:256	arg1	cavity					276:281	their internal cavity	261:281	their internal cavity	261:281	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	2	30	theme	alkyl	398:402	arg1	chains					427:432	alkyl grafted polysaccharide chains	398:432	alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	398:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	0	31	theme	α-Cyclodextrin	18:31	arg1	Polysaccharides					45:59	α-Cyclodextrin and Grafted Polysaccharides	18:59	α-Cyclodextrin and Grafted Polysaccharides	18:59	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	5	32	theme	head-to-tail	1153:1164	arg1	arrangement					1166:1176	a head-to-tail arrangement	1151:1176	a head-to-tail arrangement of the CDs	1151:1187	The monoclinic symmetry of columnar-type crystals suggests a head-to-tail arrangement of the CDs.					
29624401	8	33	theme	Melting	1389:1395	arg1	temperatures					1397:1408	Melting temperatures	1389:1408	Melting temperatures of platelets in aqueous solutions	1389:1442	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	6	34	theme	hexagonal	1226:1234	arg1	shape					1236:1240	a preferentially hexagonal shape	1209:1240	a preferentially hexagonal shape	1209:1240	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	8	35	theme	platelets	1413:1421	arg1	temperatures					1397:1408	Melting temperatures	1389:1408	Melting temperatures of platelets in aqueous solutions	1389:1442	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	3	36	with	investigation	612:624	arg1	compositions					654:665	different compositions	644:665	different compositions	644:665	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	0	37	theme	Polysaccharides	45:59	arg1	Autoassemblies					0:13	Autoassemblies	0:13	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.	0:120	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	8	38	from	temperatures	1397:1408	arg1	solutions					1434:1442	aqueous solutions	1426:1442	aqueous solutions	1426:1442	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	8	39	theme	platelet	1514:1521	arg1	structure					1523:1531	platelet structure	1514:1531	platelet structure	1514:1531	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	3	40	theme	micro-differential	903:920	arg1	calorimetry					931:941	micro-differential scanning calorimetry	903:941	micro-differential scanning calorimetry	903:941	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	2	41	theme	CDs	389:391	arg1	inclusions					373:382	inclusions	373:382	inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	373:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	0	42	theme	Grafted	37:43	arg1	Polysaccharides					45:59	α-Cyclodextrin and Grafted Polysaccharides	18:59	α-Cyclodextrin and Grafted Polysaccharides	18:59	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	3	43	theme	X-ray	765:769	arg1	structure					747:755	crystalline structure	735:755	crystalline structure (powder X-ray diffraction)	735:782	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	43	theme	X-ray	765:769	arg1	diffraction					771:781	powder X-ray diffraction	758:781	powder X-ray diffraction	758:781	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	44	theme	electron	817:824	arg1	gun					852:854	scanning electron microscopy-field emission gun	808:854	scanning electron microscopy-field emission gun	808:854	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	44	theme	electron	817:824	arg1	dimensions					785:794	dimensions	785:794	dimensions	785:794	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	5	45	theme	monoclinic	1096:1105	arg1	symmetry					1107:1114	The monoclinic symmetry	1092:1114	The monoclinic symmetry of columnar-type crystals	1092:1140	The monoclinic symmetry of columnar-type crystals suggests a head-to-tail arrangement of the CDs.					
29624401	6	46	theme	variable	1260:1267	arg1	sizes					1269:1273	variable sizes	1260:1273	variable sizes	1260:1273	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	2	47	theme	chondroitin	477:487	arg1	sulfate					489:495	chondroitin sulfate	477:495	chondroitin sulfate	477:495	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	47	theme	chondroitin	477:487	arg1	pullulan					435:442	pullulan	435:442	pullulan	435:442	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	0	48	theme	Crystal	62:68	arg1	Structure					70:78	Crystal Structure	62:78	Crystal Structure	62:78	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	5	49	theme	CDs	1185:1187	arg1	arrangement					1166:1176	a head-to-tail arrangement	1151:1176	a head-to-tail arrangement of the CDs	1151:1187	The monoclinic symmetry of columnar-type crystals suggests a head-to-tail arrangement of the CDs.					
29624401	3	50	theme	extensive	602:610	arg1	investigation					612:624	an extensive investigation	599:624	an extensive investigation of platelets with different compositions	599:665	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	4	51	theme	polysaccharide	1016:1029	arg1	backbones					1031:1039	the polysaccharide backbones	1012:1039	the polysaccharide backbones	1012:1039	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
29624401	1	52	theme	hydrophobic	296:306	arg1	environment					308:318	a hydrophobic environment	294:318	a hydrophobic environment for poorly soluble molecules	294:347	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	7	53	theme	crystal	1345:1351	arg1	parameters					1353:1362	The crystal parameters	1341:1362	The crystal parameters	1341:1362	The crystal parameters change upon dehydration.					
29624401	3	54	theme	different	644:652	arg1	compositions					654:665	different compositions	644:665	different compositions	644:665	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	55	theme	powder	758:763	arg1	structure					747:755	crystalline structure	735:755	crystalline structure (powder X-ray diffraction)	735:782	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	55	theme	powder	758:763	arg1	diffraction					771:781	powder X-ray diffraction	758:781	powder X-ray diffraction	758:781	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	1	56	with	oligosaccharides	158:173	arg1	shape					189:193	a toroid shape	180:193	a toroid shape	180:193	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	6	57	theme	sharp	1247:1251	arg1	edges					1253:1257	sharp edges	1247:1257	sharp edges	1247:1257	The platelets have a preferentially hexagonal shape with sharp edges, variable sizes, and thicknesses and sometimes show incomplete layers (terraces).					
29624401	8	58	theme	aqueous	1426:1432	arg1	solutions					1434:1442	aqueous solutions	1426:1442	aqueous solutions	1426:1442	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	3	59	theme	platelets	629:637	arg1	investigation					612:624	an extensive investigation	599:624	an extensive investigation of platelets with different compositions	599:665	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	8	60	dep	°C.	1455:1457	arg1	discuss					1471:1477	discuss	1471:1477	discuss the potential relation between the platelet structure and applications for mucoadhesive devices	1471:1573	Melting temperatures of platelets in aqueous solutions exceed 100 °C. Finally, we discuss the potential relation between the platelet structure and applications for mucoadhesive devices.					
29624401	3	61	theme	microscopy-field	826:841	arg1	gun					852:854	scanning electron microscopy-field emission gun	808:854	scanning electron microscopy-field emission gun	808:854	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	61	theme	microscopy-field	826:841	arg1	dimensions					785:794	dimensions	785:794	dimensions	785:794	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	1	62	theme	remarkable	212:221	arg1	ability					223:229	a remarkable ability	210:229	a remarkable ability to include guest molecules in their internal cavity	210:281	Cyclodextrins (CDs) are a family of oligosaccharides with a toroid shape, which exhibit a remarkable ability to include guest molecules in their internal cavity, providing a hydrophobic environment for poorly soluble molecules.					
29624401	2	63	theme	α	387:387	arg1	CDs					389:391	α CDs	387:391	α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	387:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	64	theme	grafted	404:410	arg1	chains					427:432	alkyl grafted polysaccharide chains	398:432	alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	398:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	2	65	theme	new	360:362	arg1	types					364:368	new types	360:368	new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...)	360:499	Recently, new types of inclusions of α CDs with alkyl grafted polysaccharide chains (pullulan, chitosan, dextran, amylopectin, chondroitin sulfate...) have been prepared which are autoassembled into micro- and nanoplatelets.					
29624401	3	66	theme	emission	843:850	arg1	gun					852:854	scanning electron microscopy-field emission gun	808:854	scanning electron microscopy-field emission gun	808:854	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	3	66	theme	emission	843:850	arg1	dimensions					785:794	dimensions	785:794	dimensions	785:794	We report in this paper an extensive investigation of platelets with different compositions, including their reversible hydration (thermogravimetric analysis), crystalline structure (powder X-ray diffraction), dimensions and shapes (scanning electron microscopy-field emission gun), thermal properties, solubility, and melting (micro-differential scanning calorimetry).					
29624401	0	67	dep	Autoassemblies	0:13	arg1	Structure					70:78	Crystal Structure	62:78	Crystal Structure	62:78	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	0	67	dep	Autoassemblies	0:13	arg1	Properties					93:102	Specific Properties	84:102	Specific Properties of the Platelets	84:119	Autoassemblies of α-Cyclodextrin and Grafted Polysaccharides: Crystal Structure and Specific Properties of the Platelets.					
29624401	4	68	theme	grafted	1070:1076	arg1	chains					1084:1089	the grafted alkyl chains	1066:1089	the grafted alkyl chains	1066:1089	The crystalline platelets exhibit layered structures intercalating the polysaccharide backbones and CD complexes hosting the grafted alkyl chains.					
30872059	5	0	theme	experimental	758:769	arg1	dressings					794:802	the experimental biotechnological wound dressings	754:802	the experimental biotechnological wound dressings	754:802	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	2	1	theme	experimental	326:337	arg1	membranes					339:347	the experimental membranes	322:347	the experimental membranes	322:347	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	1	2	theme	acids	248:252	arg1	cellulose					178:186	bacterial cellulose	168:186	bacterial cellulose (BC)	168:191	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	2	theme	acids	248:252	arg1	biomaterials					154:165	two biomaterials	150:165	two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	150:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	2	theme	acids	248:252	arg1	BC					189:190	BC	189:190	BC	189:190	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	2	theme	acids	248:252	arg1	copolymer					197:205	copolymer	197:205	copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	197:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	5	3	theme	biotechnological	771:786	arg1	dressings					794:802	the experimental biotechnological wound dressings	754:802	the experimental biotechnological wound dressings	754:802	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	7	4	theme	commercial	1232:1241	arg1	dressing					1249:1256	a commercial wound dressing	1230:1256	a commercial wound dressing	1230:1256	Experimental wound dressings promoted healing more effectively than VoskoPran - a commercial wound dressing.					
30872059	6	5	theme	collagen	1082:1089	arg1	factors					1036:1042	detecting factors	1026:1042	detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14	1026:1112	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	6	theme	type	1075:1078	arg1	collagen					1082:1089	type I collagen	1075:1089	type I collagen	1075:1089	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	4	7	theme	in	603:604	arg1	study					612:616	The in vitro study	599:616	The in vitro study	599:616	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	7	8	theme	wound	1243:1247	arg1	dressing					1249:1256	a commercial wound dressing	1230:1256	a commercial wound dressing	1230:1256	Experimental wound dressings promoted healing more effectively than VoskoPran - a commercial wound dressing.					
30872059	6	9	theme	I	1080:1080	arg1	collagen					1082:1089	type I collagen	1075:1089	type I collagen	1075:1089	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	10	theme	histological	959:970	arg1	examination					972:982	histological examination	959:982	histological examination	959:982	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	7	11	theme	Experimental	1150:1161	arg1	dressings					1169:1177	Experimental wound dressings	1150:1177	Experimental wound dressings	1150:1177	Experimental wound dressings promoted healing more effectively than VoskoPran - a commercial wound dressing.					
30872059	5	12	with	animals	897:903	arg1	burns					934:938	model third-degree skin burns	910:938	model third-degree skin burns	910:938	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	6	13	theme	factors	1036:1042	arg1	planimetry					947:956	Wound planimetry	941:956	Wound planimetry	941:956	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	13	theme	factors	1036:1042	arg1	examination					972:982	histological examination	959:982	histological examination	959:982	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	13	theme	factors	1036:1042	arg1	methods					1015:1021	biochemical and molecular methods	989:1021	biochemical and molecular methods	989:1021	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	14	theme	wound	1135:1139	arg1	healing					1141:1147	wound healing	1135:1147	wound healing	1135:1147	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	4	15	theme	growing	663:669	arg1	fibroblasts					671:681	growing fibroblasts	663:681	growing fibroblasts	663:681	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	5	16	theme	BC/P	806:809	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	16	theme	BC/P	806:809	arg1	/actovegin					819:828	BC/P(3HB/4HB)/actovegin	806:828	BC/P(3HB/4HB)/actovegin	806:828	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	17	theme	wound	788:792	arg1	dressings					794:802	the experimental biotechnological wound dressings	754:802	the experimental biotechnological wound dressings	754:802	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	18	theme	3HB/4HB	811:817	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	18	theme	3HB/4HB	811:817	arg1	/actovegin					819:828	BC/P(3HB/4HB)/actovegin	806:828	BC/P(3HB/4HB)/actovegin	806:828	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	6	19	used	used	1119:1122	arg2	methods					1015:1021	biochemical and molecular methods	989:1021	biochemical and molecular methods	989:1021	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	19	used	used	1119:1122	arg2	planimetry					947:956	Wound planimetry	941:956	Wound planimetry	941:956	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	6	19	used	used	1119:1122	arg2	examination					972:982	histological examination	959:982	histological examination	959:982	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	1	20	theme	bacterial	168:176	arg1	cellulose					178:186	bacterial cellulose	168:186	bacterial cellulose (BC)	168:191	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	20	theme	bacterial	168:176	arg1	BC					189:190	BC	189:190	BC	189:190	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	20	theme	bacterial	168:176	arg1	biomaterials					154:165	two biomaterials	150:165	two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	150:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	2	21	theme	multipotent	436:446	arg1	cells					481:485	multipotent adipose-derived mesenchymal stem cells	436:485	multipotent adipose-derived mesenchymal stem cells	436:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	2	22	link	adipose-derived	448:462	arg1	cells					481:485	multipotent adipose-derived mesenchymal stem cells	436:485	multipotent adipose-derived mesenchymal stem cells	436:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	5	23	theme	model	910:914	arg1	burns					934:938	model third-degree skin burns	910:938	model third-degree skin burns	910:938	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	0	24	theme	Biotechnological	0:15	arg1	dressings					23:31	Biotechnological wound dressings	0:31	Biotechnological wound dressings	0:31	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	1	25	dep	biomaterials	154:165	arg1	cellulose					178:186	bacterial cellulose	168:186	bacterial cellulose (BC)	168:191	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	25	dep	biomaterials	154:165	arg1	biomaterials					154:165	two biomaterials	150:165	two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	150:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	25	dep	biomaterials	154:165	arg1	BC					189:190	BC	189:190	BC	189:190	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	25	dep	biomaterials	154:165	arg1	copolymer					197:205	copolymer	197:205	copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	197:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	6	26	theme	angiogenesis	1047:1058	arg1	factors					1036:1042	detecting factors	1026:1042	detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14	1026:1112	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	5	27	theme	skin	929:932	arg1	burns					934:938	model third-degree skin burns	910:938	model third-degree skin burns	910:938	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	2	28	theme	epidermal	400:408	arg1	cells					410:414	epidermal cells	400:414	epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells	400:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	5	29	theme	third-degree	916:927	arg1	burns					934:938	model third-degree skin burns	910:938	model third-degree skin burns	910:938	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	6	30	theme	Wound	941:945	arg1	planimetry					947:956	Wound planimetry	941:956	Wound planimetry	941:956	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	4	31	dep	in	603:604	arg1	vitro					606:610	vitro	606:610	vitro	606:610	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	1	32	theme	Hybrid	99:104	arg1	dressings					112:120	Hybrid wound dressings	99:120	Hybrid wound dressings	99:120	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	5	33	theme	BC/P	834:837	arg1	/fibroblasts					847:858	BC/P(3HB/4HB)/fibroblasts	834:858	BC/P(3HB/4HB)/fibroblasts	834:858	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	33	theme	BC/P	834:837	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	0	34	theme	bacterial	42:50	arg1	cellulose					52:60	bacterial cellulose	42:60	bacterial cellulose	42:60	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	7	35	theme	wound	1163:1167	arg1	dressings					1169:1177	Experimental wound dressings	1150:1177	Experimental wound dressings	1150:1177	Experimental wound dressings promoted healing more effectively than VoskoPran - a commercial wound dressing.					
30872059	6	36	theme	detecting	1026:1034	arg1	factors					1036:1042	detecting factors	1026:1042	detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14	1026:1112	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	2	37	theme	stem	476:479	arg1	cells					481:485	multipotent adipose-derived mesenchymal stem cells	436:485	multipotent adipose-derived mesenchymal stem cells	436:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	1	38	theme	biodegradable	271:283	arg1	polymer					285:291	a biodegradable polymer	269:291	a biodegradable polymer of microbial origin	269:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	5	39	theme	dressings	794:802	arg1	/fibroblasts					847:858	BC/P(3HB/4HB)/fibroblasts	834:858	BC/P(3HB/4HB)/fibroblasts	834:858	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	39	theme	dressings	794:802	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	39	theme	dressings	794:802	arg1	/actovegin					819:828	BC/P(3HB/4HB)/actovegin	806:828	BC/P(3HB/4HB)/actovegin	806:828	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	40	theme	3HB/4HB	839:845	arg1	/fibroblasts					847:858	BC/P(3HB/4HB)/fibroblasts	834:858	BC/P(3HB/4HB)/fibroblasts	834:858	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	40	theme	3HB/4HB	839:845	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	2	41	theme	wound	382:386	arg1	healing					388:394	wound healing	382:394	wound healing	382:394	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	2	42	theme	mesenchymal	464:474	arg1	cells					481:485	multipotent adipose-derived mesenchymal stem cells	436:485	multipotent adipose-derived mesenchymal stem cells	436:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	3	43	theme	membranes	588:596	arg1	structure					536:544	structure	536:544	structure	536:544	A study has been carried out to investigate the structure and physical/mechanical properties of the membranes.					
30872059	3	43	theme	membranes	588:596	arg1	physical/mechanical					550:568	physical/mechanical	550:568	physical/mechanical	550:568	A study has been carried out to investigate the structure and physical/mechanical properties of the membranes.					
30872059	6	44	theme	keratin	1096:1102	arg1	factors					1036:1042	detecting factors	1026:1042	detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14	1026:1112	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	1	45	theme	wound	106:110	arg1	dressings					112:120	Hybrid wound dressings	99:120	Hybrid wound dressings	99:120	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	2	46	theme	adipose-derived	448:462	arg1	cells					481:485	multipotent adipose-derived mesenchymal stem cells	436:485	multipotent adipose-derived mesenchymal stem cells	436:485	Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells.					
30872059	5	47	dep	types	745:749	arg1	/fibroblasts					847:858	BC/P(3HB/4HB)/fibroblasts	834:858	BC/P(3HB/4HB)/fibroblasts	834:858	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	47	dep	types	745:749	arg1	types					745:749	Two types	741:749	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts -	741:860	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	47	dep	types	745:749	arg1	/actovegin					819:828	BC/P(3HB/4HB)/actovegin	806:828	BC/P(3HB/4HB)/actovegin	806:828	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	5	48	theme	laboratory	886:895	arg1	animals					897:903	laboratory animals	886:903	laboratory animals with model third-degree skin burns	886:938	Two types of the experimental biotechnological wound dressings - BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts - were tested in vivo, on laboratory animals with model third-degree skin burns.					
30872059	1	49	theme	microbial	296:304	arg1	origin					306:311	microbial origin	296:311	microbial origin	296:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	6	50	theme	molecular	1005:1013	arg1	methods					1015:1021	biochemical and molecular methods	989:1021	biochemical and molecular methods	989:1021	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	3	51	dep	structure	536:544	arg1	properties					570:579	properties	570:579	properties	570:579	A study has been carried out to investigate the structure and physical/mechanical properties of the membranes.					
30872059	3	51	dep	structure	536:544	arg1	the					532:534	the	532:534	the	532:534	A study has been carried out to investigate the structure and physical/mechanical properties of the membranes.					
30872059	1	52	dep	acids	248:252	arg1	[P					254:255	[P	254:255	[P(3HB/4HB)]	254:265	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	52	dep	acids	248:252	arg1	3HB/4HB					257:263	3HB/4HB	257:263	3HB/4HB	257:263	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	1	52	dep	acids	248:252	arg1	polymer					285:291	a biodegradable polymer	269:291	a biodegradable polymer of microbial origin	269:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	4	53	theme	composite	688:696	arg1	3HB/4HB					703:709	3HB/4HB	703:709	3HB/4HB	703:709	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	4	53	theme	composite	688:696	arg1	BC/P					698:701	composite BC/P	688:701	composite BC/P(3HB/4HB) films loaded with actovegin	688:738	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	1	54	theme	3-hydroxybutyric	210:225	arg1	acids					248:252	3-hydroxybutyric and 4-hydroxybutyric acids	210:252	3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	210:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	0	55	theme	copolymer	77:85	arg1	P					87:87	degradable copolymer P	66:87	degradable copolymer P(3HB/4HB)	66:96	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	0	55	theme	copolymer	77:85	arg1	3HB/4HB					89:95	3HB/4HB	89:95	3HB/4HB	89:95	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	4	56	theme	effective	639:647	arg1	films					712:716	composite BC/P(3HB/4HB) films	688:716	composite BC/P(3HB/4HB) films loaded with actovegin	688:738	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	4	56	theme	effective	639:647	arg1	scaffolds					649:657	the most effective scaffolds	630:657	the most effective scaffolds for growing fibroblasts	630:681	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	4	57	theme	BC/P	698:701	arg1	films					712:716	composite BC/P(3HB/4HB) films	688:716	composite BC/P(3HB/4HB) films loaded with actovegin	688:738	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	4	57	theme	BC/P	698:701	arg1	scaffolds					649:657	the most effective scaffolds	630:657	the most effective scaffolds for growing fibroblasts	630:681	The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin.					
30872059	6	58	theme	inflammation	1061:1072	arg1	factors					1036:1042	detecting factors	1026:1042	detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14	1026:1112	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	0	59	theme	degradable	66:75	arg1	P					87:87	degradable copolymer P	66:87	degradable copolymer P(3HB/4HB)	66:96	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	0	59	theme	degradable	66:75	arg1	3HB/4HB					89:95	3HB/4HB	89:95	3HB/4HB	89:95	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	1	60	theme	origin	306:311	arg1	polymer					285:291	a biodegradable polymer	269:291	a biodegradable polymer of microbial origin	269:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
30872059	6	61	theme	biochemical	989:999	arg1	methods					1015:1021	biochemical and molecular methods	989:1021	biochemical and molecular methods	989:1021	Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing.					
30872059	0	62	theme	wound	17:21	arg1	dressings					23:31	Biotechnological wound dressings	0:31	Biotechnological wound dressings	0:31	Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB).					
30872059	1	63	theme	4-hydroxybutyric	231:246	arg1	acids					248:252	3-hydroxybutyric and 4-hydroxybutyric acids	210:252	3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin	210:311	Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] - a biodegradable polymer of microbial origin.					
29370305	12	0	theme	virus	2046:2050	arg1	resistance					2052:2061	virus resistance	2046:2061	virus resistance to the host antibody responses	2046:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	7	1	theme	virus	1240:1244	arg1	capture					1246:1252	DC-SIGN-mediated virus capture	1223:1252	DC-SIGN-mediated virus capture	1223:1252	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	4	2	theme	SP	552:553	arg1	sequences					555:563	The Env SP sequences	544:563	The Env SP sequences of HIV-1 isolates	544:581	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	2	3	theme	nascent	322:328	arg1	Env					330:332	the nascent Env	318:332	the nascent Env	318:332	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	5	4	from	changes	689:695	arg1	SP					708:709	the Env SP	700:709	the Env SP	700:709	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	10	5	theme	Increased	1576:1584	arg1	resistance					1601:1610	Increased neutralization resistance	1576:1610	Increased neutralization resistance	1576:1610	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	12	6	theme	balance	2012:2018	arg1	infectiousness					2026:2039	balance virus infectiousness	2012:2039	balance virus infectiousness with virus resistance to the host antibody responses	2012:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	11	7	theme	oligosaccharide	1898:1912	arg1	moieties					1914:1921	oligosaccharide moieties	1898:1921	oligosaccharide moieties on the Env N-glycans	1898:1942	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	5	8	theme	ER-Golgi	747:754	arg1	pathway					766:772	the ER-Golgi secretory pathway	743:772	the ER-Golgi secretory pathway	743:772	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	7	9	theme	different	1072:1080	arg1	mutations					1082:1090	three different mutations	1066:1090	three different mutations affecting histidine at position 12	1066:1125	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	4	10	theme	such	642:645	arg1	variability					647:657	such variability	642:657	such variability	642:657	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	10	11	theme	N-glycan	1616:1623	arg1	composition					1625:1635	N-glycan composition	1616:1635	N-glycan composition	1616:1635	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	11	12	theme	certain	1803:1809	arg1	residues					1811:1818	certain residues	1803:1818	certain residues in the HIV-1 Env SP	1803:1838	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	9	13	theme	oligosaccharide	1449:1463	arg1	composition					1465:1475	the oligosaccharide composition	1445:1475	the oligosaccharide composition of N-glycans	1445:1488	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	3	14	theme	protein	535:541	arg1	traits					517:522	the phenotypic traits	502:522	the phenotypic traits of the Env protein	502:541	SP is cleaved during Env biosynthesis but potentially influences the phenotypic traits of the Env protein.					
29370305	12	15	theme	Env	1955:1957	arg1	sequences					1962:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	11	16	theme	HIV-1	1827:1831	arg1	SP					1837:1838	the HIV-1 Env SP	1823:1838	the HIV-1 Env SP	1823:1838	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	12	17	with	infectiousness	2026:2039	arg1	resistance					2052:2061	virus resistance	2046:2061	virus resistance to the host antibody responses	2046:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	5	18	from	impact	816:821	arg1	incorporation					830:842	Env incorporation	826:842	Env incorporation into virions	826:855	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	8	19	from	positions	1285:1293	arg1	Mutations					1272:1280	Mutations	1272:1280	Mutations at positions 8, 12, and 15	1272:1307	Mutations at positions 8, 12, and 15 also rendered the virus more resistant to neutralization by monoclonal antibodies against the Env V1V2 region.					
29370305	11	20	from	moieties	1914:1921	arg1	N-glycans					1934:1942	the Env N-glycans	1926:1942	the Env N-glycans	1926:1942	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	7	21	theme	DC-SIGN-mediated	1223:1238	arg1	capture					1246:1252	DC-SIGN-mediated virus capture	1223:1252	DC-SIGN-mediated virus capture	1223:1252	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	2	22	theme	HIV-1	246:250	arg1	Env					252:254	HIV-1 Env	246:254	HIV-1 Env	246:254	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	5	23	theme	receptor	858:865	arg1	binding					867:873	receptor binding	858:873	receptor binding	858:873	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	12	24	theme	host	2070:2073	arg1	responses					2084:2092	the host antibody responses	2066:2092	the host antibody responses	2066:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	11	25	theme	first	1778:1782	arg1	this					1766:1769	this	1766:1769	this	1766:1769	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	11	25	theme	first	1778:1782	arg1	study					1784:1788	the first study	1774:1788	the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans	1774:1942	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	0	26	theme	peptide	87:93	arg1	mutations					95:103	signal peptide mutations	80:103	signal peptide mutations	80:103	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	5	27	from	binding	867:873	arg1	incorporation					830:842	Env incorporation	826:842	Env incorporation into virions	826:855	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	9	28	theme	specific	1533:1540	arg1	lectins					1542:1548	specific lectins	1533:1548	specific lectins	1533:1548	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	8	29	theme	resistant	1338:1346	arg1	virus					1327:1331	the virus	1323:1331	the virus more resistant to neutralization	1323:1364	Mutations at positions 8, 12, and 15 also rendered the virus more resistant to neutralization by monoclonal antibodies against the Env V1V2 region.					
29370305	9	30	theme	mass	1557:1560	arg1	spectrometry					1562:1573	mass spectrometry	1557:1573	mass spectrometry	1557:1573	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	10	31	theme	analogous	1669:1677	arg1	mutations					1679:1687	analogous mutations	1669:1687	analogous mutations	1669:1687	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	5	32	theme	Env	778:780	arg1	folding					782:788	Env folding	778:788	Env folding	778:788	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	1	33	theme	HIV-1	106:110	arg1	Env					135:137	Env	135:137	Env	135:137	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	1	33	theme	HIV-1	106:110	arg1	glycoprotein					121:132	HIV-1 envelope glycoprotein	106:132	HIV-1 envelope glycoprotein (Env)	106:138	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	6	34	theme	HIV-1	1029:1033	arg1	REJO.c/2864					1035:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	2	35	theme	post-translational	392:409	arg1	modifications					411:423	post-translational modifications	392:423	post-translational modifications	392:423	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	8	36	theme	monoclonal	1369:1378	arg1	antibodies					1380:1389	monoclonal antibodies	1369:1389	monoclonal antibodies against the Env V1V2 region	1369:1417	Mutations at positions 8, 12, and 15 also rendered the virus more resistant to neutralization by monoclonal antibodies against the Env V1V2 region.					
29370305	10	37	theme	HIV-1	1716:1720	arg1	JRFL					1730:1733	JRFL	1730:1733	JRFL	1730:1733	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	10	37	theme	HIV-1	1716:1720	arg1	strain					1722:1727	another HIV-1 strain	1708:1727	another HIV-1 strain	1708:1727	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	0	38	theme	HIV-1	15:19	arg1	phenotype					30:38	HIV-1 envelope phenotype	15:38	HIV-1 envelope phenotype	15:38	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	3	39	theme	phenotypic	506:515	arg1	traits					517:522	the phenotypic traits	502:522	the phenotypic traits of the Env protein	502:541	SP is cleaved during Env biosynthesis but potentially influences the phenotypic traits of the Env protein.					
29370305	6	40	theme	molecular	1013:1021	arg1	REJO.c/2864					1035:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	11	41	theme	Env	1930:1932	arg1	N-glycans					1934:1942	the Env N-glycans	1926:1942	the Env N-glycans	1926:1942	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	7	42	theme	Env	1136:1138	arg1	incorporation					1140:1152	Env incorporation	1136:1152	Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission	1136:1269	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	0	43	theme	phenotype	30:38	arg1	Alterations					0:10	Alterations	0:10	Alterations of HIV-1 envelope phenotype	0:38	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	0	43	theme	phenotype	30:38	arg1	neutralization					62:75	antibody-mediated neutralization	44:75	antibody-mediated neutralization by signal peptide mutations	44:103	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	5	44	theme	Env	721:723	arg1	transport					725:733	Env transport	721:733	Env transport through the ER-Golgi secretory pathway	721:772	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	8	45	theme	Env	1403:1405	arg1	region					1412:1417	the Env V1V2 region	1399:1417	the Env V1V2 region	1399:1417	Mutations at positions 8, 12, and 15 also rendered the virus more resistant to neutralization by monoclonal antibodies against the Env V1V2 region.					
29370305	3	46	theme	Env	458:460	arg1	biosynthesis					462:473	Env biosynthesis	458:473	Env biosynthesis	458:473	SP is cleaved during Env biosynthesis but potentially influences the phenotypic traits of the Env protein.					
29370305	0	47	theme	antibody-mediated	44:60	arg1	neutralization					62:75	antibody-mediated neutralization	44:75	antibody-mediated neutralization by signal peptide mutations	44:103	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	10	48	dep	resistance	1601:1610	arg1	changes					1637:1643	changes	1637:1643	changes	1637:1643	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	2	49	theme	other	202:206	arg1	proteins					236:243	other membrane-bound and secreted proteins	202:243	other membrane-bound and secreted proteins	202:243	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	4	50	theme	HIV-1	568:572	arg1	isolates					574:581	HIV-1 isolates	568:581	HIV-1 isolates	568:581	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	12	51	theme	virus	2020:2024	arg1	infectiousness					2026:2039	balance virus infectiousness	2012:2039	balance virus infectiousness with virus resistance to the host antibody responses	2012:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	11	52	theme	neutralization	1857:1870	arg1	sensitivity					1872:1882	virus neutralization sensitivity	1851:1882	virus neutralization sensitivity	1851:1882	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	4	53	theme	Env	548:550	arg1	sequences					555:563	The Env SP sequences	544:563	The Env SP sequences of HIV-1 isolates	544:581	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	10	54	theme	neutralization	1586:1599	arg1	resistance					1601:1610	Increased neutralization resistance	1576:1610	Increased neutralization resistance	1576:1610	Increased neutralization resistance and N-glycan composition changes were also observed when analogous mutations were introduced to another HIV-1 strain, JRFL.					
29370305	2	55	contain	contains	256:263	arg1	terminus					274:281	its N terminus	268:281	its N terminus	268:281	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	55	contain	contains	256:263	arg1	Env					252:254	HIV-1 Env	246:254	HIV-1 Env	246:254	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	55	contain	contains	256:263	arg2	peptide					292:298	a signal peptide	283:298	a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place	283:434	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	55	contain	contains	256:263	arg2	Env					252:254	HIV-1 Env	246:254	HIV-1 Env	246:254	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	55	contain	contains	256:263	arg2	SP					301:302	SP	301:302	SP	301:302	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	12	56	theme	selective	1990:1998	arg1	pressure					2000:2007	selective pressure	1990:2007	selective pressure	1990:2007	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	2	57	theme	N	272:272	arg1	terminus					274:281	its N terminus	268:281	its N terminus	268:281	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	5	58	theme	Env	826:828	arg1	incorporation					830:842	Env incorporation	826:842	Env incorporation into virions	826:855	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	9	59	from	changes	1502:1508	arg1	reactivity					1517:1526	Env reactivity	1513:1526	Env reactivity with specific lectins	1513:1548	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	7	60	theme	infectivity	1207:1217	arg1	reduction					1188:1196	reduction	1188:1196	reduction of virus infectivity	1188:1217	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	7	60	theme	infectivity	1207:1217	arg1	transmission					1258:1269	transmission	1258:1269	transmission	1258:1269	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	7	60	theme	infectivity	1207:1217	arg1	capture					1246:1252	DC-SIGN-mediated virus capture	1223:1252	DC-SIGN-mediated virus capture	1223:1252	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	1	61	theme	host	185:188	arg1	cells					190:194	the host cells	181:194	the host cells	181:194	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	6	62	theme	charged	953:959	arg1	residues					961:968	the charged residues	949:968	the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864	949:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	11	63	from	residues	1811:1818	arg1	SP					1837:1838	the HIV-1 Env SP	1823:1838	the HIV-1 Env SP	1823:1838	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	3	64	theme	Env	531:533	arg1	protein					535:541	the Env protein	527:541	the Env protein	527:541	SP is cleaved during Env biosynthesis but potentially influences the phenotypic traits of the Env protein.					
29370305	4	65	theme	sequence	596:603	arg1	variability					605:615	high sequence variability	591:615	high sequence variability	591:615	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	9	66	theme	N-glycans	1480:1488	arg1	composition					1465:1475	the oligosaccharide composition	1445:1475	the oligosaccharide composition of N-glycans	1445:1488	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	12	67	theme	HIV-1	1949:1953	arg1	sequences					1962:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	11	68	theme	Env	1833:1835	arg1	SP					1837:1838	the HIV-1 Env SP	1823:1838	the HIV-1 Env SP	1823:1838	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	2	69	theme	secreted	227:234	arg1	proteins					236:243	other membrane-bound and secreted proteins	202:243	other membrane-bound and secreted proteins	202:243	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	5	70	theme	antibody	879:886	arg1	recognition					888:898	antibody recognition	879:898	antibody recognition	879:898	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	12	71	theme	SP	1959:1960	arg1	sequences					1962:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences	1945:1970	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	12	72	theme	antibody	2075:2082	arg1	responses					2084:2092	the host antibody responses	2066:2092	the host antibody responses	2066:2092	The HIV-1 Env SP sequences thus may be under selective pressure to balance virus infectiousness with virus resistance to the host antibody responses.					
29370305	0	73	theme	signal	80:85	arg1	mutations					95:103	signal peptide mutations	80:103	signal peptide mutations	80:103	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	9	74	theme	Env	1513:1515	arg1	reactivity					1517:1526	Env reactivity	1513:1526	Env reactivity with specific lectins	1513:1548	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	7	75	from	position	1115:1122	arg1	histidine					1102:1110	histidine	1102:1110	histidine at position 12	1102:1125	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	4	76	theme	variability	647:657	arg1	unclear					662:668	unclear	662:668	unclear	662:668	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	4	76	theme	variability	647:657	arg1	significance					626:637	the significance	622:637	the significance of such variability	622:657	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	7	77	theme	virus	1201:1205	arg1	infectivity					1207:1217	virus infectivity	1201:1217	virus infectivity	1201:1217	Results show that three different mutations affecting histidine at position 12 affected Env incorporation into virions that correlated with reduction of virus infectivity and DC-SIGN-mediated virus capture and transmission.					
29370305	6	78	theme	Env	977:979	arg1	SP					981:982	the Env SP	973:982	the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864	973:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	6	79	from	SP	981:982	arg1	context					991:997	the context	987:997	the context of infectious molecular clone HIV-1 REJO.c/2864	987:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	2	80	theme	signal	285:290	arg1	peptide					292:298	a signal peptide	283:298	a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place	283:434	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	80	theme	signal	285:290	arg1	SP					301:302	SP	301:302	SP	301:302	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	9	81	with	reactivity	1517:1526	arg1	lectins					1542:1548	specific lectins	1533:1548	specific lectins	1533:1548	These mutations affected the oligosaccharide composition of N-glycans as shown by changes in Env reactivity with specific lectins and by mass spectrometry.					
29370305	1	82	gly	glycoprotein	121:132	arg1	Env					135:137	Env	135:137	Env	135:137	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	1	82	gly	glycoprotein	121:132	arg1	glycoprotein					121:132	HIV-1 envelope glycoprotein	106:132	HIV-1 envelope glycoprotein (Env)	106:138	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	6	83	from	residues	961:968	arg1	SP					981:982	the Env SP	973:982	the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864	973:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	5	84	theme	Env	704:706	arg1	SP					708:709	the Env SP	700:709	the Env SP	700:709	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	6	85	theme	REJO.c/2864	1035:1045	arg1	context					991:997	the context	987:997	the context of infectious molecular clone HIV-1 REJO.c/2864	987:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	2	86	theme	endoplasmic	341:351	arg1	ER					364:365	ER	364:365	ER	364:365	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	2	86	theme	endoplasmic	341:351	arg1	reticulum					353:361	the endoplasmic reticulum	337:361	the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place	337:434	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	0	87	theme	envelope	21:28	arg1	phenotype					30:38	HIV-1 envelope phenotype	15:38	HIV-1 envelope phenotype	15:38	Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations.					
29370305	5	88	from	recognition	888:898	arg1	incorporation					830:842	Env incorporation	826:842	Env incorporation into virions	826:855	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	1	89	theme	envelope	112:119	arg1	Env					135:137	Env	135:137	Env	135:137	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	1	89	theme	envelope	112:119	arg1	glycoprotein					121:132	HIV-1 envelope glycoprotein	106:132	HIV-1 envelope glycoprotein (Env)	106:138	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	6	90	theme	clone	1023:1027	arg1	REJO.c/2864					1035:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	2	91	theme	Env	374:376	arg1	synthesis					378:386	Env synthesis	374:386	Env synthesis	374:386	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	6	92	theme	infectious	1002:1011	arg1	REJO.c/2864					1035:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	infectious molecular clone HIV-1 REJO.c/2864	1002:1045	We first evaluated the consequences of mutating the charged residues in the Env SP in the context of infectious molecular clone HIV-1 REJO.c/2864.					
29370305	5	93	theme	secretory	756:764	arg1	pathway					766:772	the ER-Golgi secretory pathway	743:772	the ER-Golgi secretory pathway	743:772	We postulate that changes in the Env SP influence Env transport through the ER-Golgi secretory pathway and Env folding and/or glycosylation that impact on Env incorporation into virions, receptor binding and antibody recognition.					
29370305	2	94	theme	membrane-bound	208:221	arg1	proteins					236:243	other membrane-bound and secreted proteins	202:243	other membrane-bound and secreted proteins	202:243	Like other membrane-bound and secreted proteins, HIV-1 Env contains at its N terminus a signal peptide (SP) that directs the nascent Env to the endoplasmic reticulum (ER) where Env synthesis and post-translational modifications take place.					
29370305	11	95	theme	virus	1851:1855	arg1	sensitivity					1872:1882	virus neutralization sensitivity	1851:1882	virus neutralization sensitivity	1851:1882	To the best of our knowledge, this is the first study showing that certain residues in the HIV-1 Env SP can affect virus neutralization sensitivity by modulating oligosaccharide moieties on the Env N-glycans.					
29370305	1	96	theme	virus	149:153	arg1	attachment					155:164	virus attachment	149:164	virus attachment	149:164	HIV-1 envelope glycoprotein (Env) mediates virus attachment and entry into the host cells.					
29370305	4	97	theme	isolates	574:581	arg1	sequences					555:563	The Env SP sequences	544:563	The Env SP sequences of HIV-1 isolates	544:581	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
29370305	8	98	theme	V1V2	1407:1410	arg1	region					1412:1417	the Env V1V2 region	1399:1417	the Env V1V2 region	1399:1417	Mutations at positions 8, 12, and 15 also rendered the virus more resistant to neutralization by monoclonal antibodies against the Env V1V2 region.					
29370305	4	99	theme	high	591:594	arg1	variability					605:615	high sequence variability	591:615	high sequence variability	591:615	The Env SP sequences of HIV-1 isolates display high sequence variability, and the significance of such variability is unclear.					
31703760	9	0	theme	≈	898:898	arg1	activity					870:877	their activity	864:877	their activity	864:877	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	9	0	theme	≈	898:898	arg1	SDP3>SDP2>SDP1					900:913	SDP4 ≈ SDP3>SDP2>SDP1	893:913	SDP4 ≈ SDP3>SDP2>SDP1	893:913	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	7	1	contain	possessed	635:643	arg2	4-α-glucan					679:688	the 1, 4-α-glucan	672:688	4-α-glucan	679:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	1	contain	possessed	635:643	arg2	amount					700:705	a big amount	694:705	a big amount of the arabinogalactan	694:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	1	contain	possessed	635:643	arg2	arabinogalactan					714:728	the arabinogalactan	710:728	the arabinogalactan	710:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	1	contain	possessed	635:643	arg1	SDP3					630:633	SDP3	630:633	SDP3	630:633	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	1	contain	possessed	635:643	arg2	amount					662:667	relatively lower amount	645:667	relatively lower amount of the 1, 4-α-glucan	645:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	2	contain	contained	541:549	arg2	amount					557:562	a big amount	551:562	a big amount of the 1, 4-α-glucan	551:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	2	contain	contained	541:549	arg2	4-α-glucan					574:583	the 1, 4-α-glucan	567:583	4-α-glucan	574:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	2	contain	contained	541:549	arg2	arabinogalactan					607:621	arabinogalactan	607:621	arabinogalactan	607:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	2	contain	contained	541:549	arg2	amount					597:602	a small amount	589:602	a small amount of arabinogalactan	589:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	2	contain	contained	541:549	arg1	SDP2					536:539	SDP2	536:539	SDP2	536:539	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	0	3	theme	Saposhnikovia	90:102	arg1	divaricata					104:113	Saposhnikovia divaricata	90:113	Saposhnikovia divaricata	90:113	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	5	4	theme	molecular	307:315	arg1	weight					317:322	Their molecular weight	301:322	Their molecular weight	301:322	Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR.					
31703760	10	5	theme	antioxidant	1010:1020	arg1	activity					1022:1029	the highest antioxidant activity	998:1029	the highest antioxidant activity	998:1029	The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity.					
31703760	3	6	dep	extracted	215:223	arg1	SD					207:208	SD	207:208	SD	207:208	(SD) was extracted, fractionated and characterized in this work.					
31703760	6	7	theme	branches	526:533	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	6	7	theme	branches	526:533	arg1	amount					505:510	small amount	499:510	small amount of O-6 linked branches	499:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	9	8	theme	antioxidant	799:809	arg1	activities					811:820	antioxidant activities	799:820	antioxidant activities in both molecular and cellular levels	799:858	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	6	9	theme	linked	519:524	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	7	10	theme	arabinogalactan	607:621	arg1	4-α-glucan					574:583	the 1, 4-α-glucan	567:583	4-α-glucan	574:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	10	theme	arabinogalactan	607:621	arg1	amount					557:562	a big amount	551:562	a big amount of the 1, 4-α-glucan	551:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	10	theme	arabinogalactan	607:621	arg1	arabinogalactan					607:621	arabinogalactan	607:621	arabinogalactan	607:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	10	theme	arabinogalactan	607:621	arg1	amount					597:602	a small amount	589:602	a small amount of arabinogalactan	589:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	10	11	from	4-α-glucan	923:932	arg1	SDP1					937:940	SDP1	937:940	SDP1	937:940	The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity.					
31703760	6	12	theme	O-6	515:517	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	7	13	theme	small	591:595	arg1	4-α-glucan					574:583	the 1, 4-α-glucan	567:583	4-α-glucan	574:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	13	theme	small	591:595	arg1	arabinogalactan					607:621	arabinogalactan	607:621	arabinogalactan	607:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	13	theme	small	591:595	arg1	amount					597:602	a small amount	589:602	a small amount of arabinogalactan	589:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	14	theme	big	696:698	arg1	amount					700:705	a big amount	694:705	a big amount of the arabinogalactan	694:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	14	theme	big	696:698	arg1	arabinogalactan					714:728	the arabinogalactan	710:728	the arabinogalactan	710:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	14	theme	big	696:698	arg1	4-α-glucan					679:688	the 1, 4-α-glucan	672:688	4-α-glucan	679:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	6	15	link	linked	519:524	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	7	16	theme	4-α-glucan	574:583	arg1	4-α-glucan					574:583	the 1, 4-α-glucan	567:583	4-α-glucan	574:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	16	theme	4-α-glucan	574:583	arg1	amount					557:562	a big amount	551:562	a big amount of the 1, 4-α-glucan	551:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	16	theme	4-α-glucan	574:583	arg1	arabinogalactan					607:621	arabinogalactan	607:621	arabinogalactan	607:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	16	theme	4-α-glucan	574:583	arg1	amount					597:602	a small amount	589:602	a small amount of arabinogalactan	589:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	8	17	theme	pectic	753:758	arg1	SDP4					731:734	SDP4	731:734	SDP4	731:734	SDP4 was defined as a pectic arabinogalactan.					
31703760	8	17	theme	pectic	753:758	arg1	arabinogalactan					760:774	a pectic arabinogalactan	751:774	a pectic arabinogalactan	751:774	SDP4 was defined as a pectic arabinogalactan.					
31703760	5	18	theme	linkage	354:360	arg1	modes					362:366	linkage modes	354:366	linkage modes	354:366	Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR.					
31703760	9	19	theme	molecular	830:838	arg1	levels					853:858	both molecular and cellular levels	825:858	both molecular and cellular levels	825:858	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	11	20	theme	SDP	1162:1164	arg1	activity					1150:1157	the antioxidant activity	1134:1157	the antioxidant activity of SDP	1134:1164	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	1	21	from	herb	156:159	arg1	Polysaccharide					116:129	Polysaccharide	116:129	Polysaccharide from traditional Chinese herb	116:159	Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.)					
31703760	1	21	from	herb	156:159	arg1	divaricata					176:185	divaricata	176:185	divaricata	176:185	Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.)					
31703760	11	22	theme	antioxidant	1138:1148	arg1	activity					1150:1157	the antioxidant activity	1134:1157	the antioxidant activity of SDP	1134:1164	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	0	23	theme	structure	14:22	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	9	24	from	activities	811:820	arg1	levels					853:858	both molecular and cellular levels	825:858	both molecular and cellular levels	825:858	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	9	25	theme	cellular	844:851	arg1	levels					853:858	both molecular and cellular levels	825:858	both molecular and cellular levels	825:858	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	0	26	from	divaricata	104:113	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	0	26	from	divaricata	104:113	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	0	26	from	divaricata	104:113	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	1	27	theme	traditional	136:146	arg1	herb					156:159	traditional Chinese herb	136:159	traditional Chinese herb	136:159	Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.)					
31703760	6	28	with	4-α-glucan	483:492	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	6	28	with	4-α-glucan	483:492	arg1	amount					505:510	small amount	499:510	small amount of O-6 linked branches	499:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	11	29	theme	major	1060:1064	arg1	component					1066:1074	the major component	1056:1074	the major component	1056:1074	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	11	29	theme	major	1060:1064	arg1	arabinogalactan					1036:1050	The arabinogalactan	1032:1050	The arabinogalactan	1032:1050	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	0	30	theme	antioxidant	45:55	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	5	31	theme	structural	372:381	arg1	properties					383:392	structural properties	372:392	structural properties	372:392	Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR.					
31703760	6	32	theme	small	499:503	arg1	branches					526:533	O-6 linked branches	515:533	O-6 linked branches	515:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	6	32	theme	small	499:503	arg1	amount					505:510	small amount	499:510	small amount of O-6 linked branches	499:533	SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches.					
31703760	7	33	theme	4-α-glucan	679:688	arg1	amount					662:667	relatively lower amount	645:667	relatively lower amount of the 1, 4-α-glucan	645:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	33	theme	4-α-glucan	679:688	arg1	amount					700:705	a big amount	694:705	a big amount of the arabinogalactan	694:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	33	theme	4-α-glucan	679:688	arg1	arabinogalactan					714:728	the arabinogalactan	710:728	the arabinogalactan	710:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	33	theme	4-α-glucan	679:688	arg1	4-α-glucan					679:688	the 1, 4-α-glucan	672:688	4-α-glucan	679:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	34	theme	big	553:555	arg1	4-α-glucan					574:583	the 1, 4-α-glucan	567:583	4-α-glucan	574:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	34	theme	big	553:555	arg1	amount					557:562	a big amount	551:562	a big amount of the 1, 4-α-glucan	551:583	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	34	theme	big	553:555	arg1	arabinogalactan					607:621	arabinogalactan	607:621	arabinogalactan	607:621	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	5	35	theme	monosaccharide	325:338	arg1	compositions					340:351	monosaccharide compositions	325:351	monosaccharide compositions	325:351	Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR.					
31703760	9	36	theme	SDP4	893:896	arg1	activity					870:877	their activity	864:877	their activity	864:877	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	9	36	theme	SDP4	893:896	arg1	SDP3>SDP2>SDP1					900:913	SDP4 ≈ SDP3>SDP2>SDP1	893:913	SDP4 ≈ SDP3>SDP2>SDP1	893:913	Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1.					
31703760	1	37	theme	Chinese	148:154	arg1	herb					156:159	traditional Chinese herb	136:159	traditional Chinese herb	136:159	Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.)					
31703760	10	38	theme	highest	1002:1008	arg1	activity					1022:1029	the highest antioxidant activity	998:1029	the highest antioxidant activity	998:1029	The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity.					
31703760	11	39	theme	SDP4	1093:1096	arg1	component					1066:1074	the major component	1056:1074	the major component	1056:1074	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	11	39	theme	SDP4	1093:1096	arg1	arabinogalactan					1036:1050	The arabinogalactan	1032:1050	The arabinogalactan	1032:1050	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	11	40	theme	SDP3	1084:1087	arg1	component					1066:1074	the major component	1056:1074	the major component	1056:1074	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	11	40	theme	SDP3	1084:1087	arg1	arabinogalactan					1036:1050	The arabinogalactan	1032:1050	The arabinogalactan	1032:1050	The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.					
31703760	7	41	theme	arabinogalactan	714:728	arg1	amount					662:667	relatively lower amount	645:667	relatively lower amount of the 1, 4-α-glucan	645:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	41	theme	arabinogalactan	714:728	arg1	amount					700:705	a big amount	694:705	a big amount of the arabinogalactan	694:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	41	theme	arabinogalactan	714:728	arg1	arabinogalactan					714:728	the arabinogalactan	710:728	the arabinogalactan	710:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	41	theme	arabinogalactan	714:728	arg1	4-α-glucan					679:688	the 1, 4-α-glucan	672:688	4-α-glucan	679:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	42	theme	lower	656:660	arg1	amount					662:667	relatively lower amount	645:667	relatively lower amount of the 1, 4-α-glucan	645:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	42	theme	lower	656:660	arg1	arabinogalactan					714:728	the arabinogalactan	710:728	the arabinogalactan	710:728	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	7	42	theme	lower	656:660	arg1	4-α-glucan					679:688	the 1, 4-α-glucan	672:688	4-α-glucan	679:688	SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan.					
31703760	0	43	theme	polysaccharides	69:83	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	0	43	theme	polysaccharides	69:83	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	0	43	theme	polysaccharides	69:83	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.					
31703760	10	44	contain	had	942:944	arg1	4-α-glucan					923:932	The 1, 4-α-glucan	916:932	4-α-glucan	923:932	The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity.					
31703760	10	44	contain	had	942:944	arg2	weakest					950:956	the weakest	946:956	the weakest	946:956	The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity.					
30217647	0	0	theme	Salicornia	92:101	arg1	arabica					103:109	Salicornia arabica	92:109	Salicornia arabica	92:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	3	1	theme	arabinose	465:473	arg1	presence					453:460	the presence	449:460	the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose	449:527	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	1	2	theme	polysaccharides	159:173	arg1	profiling					120:128	A newly profiling	112:128	A newly profiling of Salicornia arabica (SAPS) polysaccharides	112:173	A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated.					
30217647	3	3	theme	acid	512:515	arg1	presence					453:460	the presence	449:460	the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose	449:527	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	2	4	contain	contained	277:285	arg2	%					389:389	3.33 ± 0.37%	378:389	3.33 ± 0.37%	378:389	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	sulfate					287:293	sulfate	287:293	sulfate (9.64 ± 0.003%)	287:309	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	%					308:308	9.64 ± 0.003%	296:308	9.64 ± 0.003%	296:308	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	%					339:339	86.33 ± 0.003%	326:339	86.33 ± 0.003%	326:339	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	carbohydrate					312:323	carbohydrate	312:323	carbohydrate (86.33 ± 0.003%)	312:340	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg1	extract					226:232	The water-soluble polysaccharide extract	193:232	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA)	193:275	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	protein					343:349	protein	343:349	protein (1.24%)	343:357	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	%					356:356	1.24%	352:356	1.24%	352:356	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	4	contain	contained	277:285	arg2	acids					371:375	uronic acids	364:375	uronic acids (3.33 ± 0.37%)	364:390	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	5	5	theme	reducer	781:787	arg1	power					789:793	reducer power	781:793	reducer power	781:793	It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.					
30217647	2	6	theme	uronic	364:369	arg1	acids					371:375	uronic acids	364:375	uronic acids (3.33 ± 0.37%)	364:390	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	6	theme	uronic	364:369	arg1	%					389:389	3.33 ± 0.37%	378:389	3.33 ± 0.37%	378:389	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	5	7	theme	important	748:756	arg1	scavenging					766:775	an important radical scavenging	745:775	an important radical scavenging	745:775	It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.					
30217647	3	8	theme	Glucuronic	501:510	arg1	acid					512:515	Glucuronic acid	501:515	Glucuronic acid	501:515	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	0	9	theme	in	9:10	arg1	capacities					48:57	in vitro antioxidant and antimicrobial capacities	9:57	in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica	9:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	3	10	theme	galactose	490:498	arg1	presence					453:460	the presence	449:460	the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose	449:527	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	4	11	theme	chromatography	696:709	arg1	analysis					717:724	Thin layer chromatography (TLC) analysis	685:724	Thin layer chromatography (TLC) analysis	685:724	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	11	theme	chromatography	696:709	arg1	chromatography					620:633	gaz chromatography	616:633	gaz chromatography	616:633	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	12	theme	Infrared	654:661	arg1	Spectrometer					663:674	Infrared Spectrometer	654:674	Infrared Spectrometer	654:674	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	0	13	theme	antioxidant	18:28	arg1	capacities					48:57	in vitro antioxidant and antimicrobial capacities	9:57	in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica	9:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	3	14	theme	glucose	521:527	arg1	presence					453:460	the presence	449:460	the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose	449:527	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	3	15	theme	monosaccharide	413:426	arg1	composition					428:438	the monosaccharide composition	409:438	the monosaccharide composition	409:438	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	4	16	theme	identified	534:543	arg1	polysaccharide					545:558	The identified polysaccharide	530:558	The identified polysaccharide	530:558	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	1	17	theme	newly	114:118	arg1	profiling					120:128	A newly profiling	112:128	A newly profiling of Salicornia arabica (SAPS) polysaccharides	112:173	A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated.					
30217647	3	18	theme	composition	428:438	arg1	analysis					397:404	The analysis	393:404	The analysis of the monosaccharide composition	393:438	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	0	19	theme	antimicrobial	34:46	arg1	capacities					48:57	in vitro antioxidant and antimicrobial capacities	9:57	in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica	9:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	5	20	theme	effective	816:824	arg1	properties					840:849	effective antimicrobial properties	816:849	effective antimicrobial properties	816:849	It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.					
30217647	5	21	theme	radical	758:764	arg1	scavenging					766:775	an important radical scavenging	745:775	an important radical scavenging	745:775	It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.					
30217647	2	22	theme	Tunisian	253:260	arg1	SA					273:274	SA	273:274	SA	273:274	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	2	22	theme	Tunisian	253:260	arg1	halophyte					262:270	the Tunisian halophyte	249:270	the Tunisian halophyte (SA)	249:275	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	5	23	theme	antimicrobial	826:838	arg1	properties					840:849	effective antimicrobial properties	816:849	effective antimicrobial properties	816:849	It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.					
30217647	0	24	attach	isolated	78:85	arg2	polysaccharides					62:76	polysaccharides	62:76	polysaccharides isolated from Salicornia arabica	62:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	0	24	attach	isolated	78:85	arg1	arabica					103:109	Salicornia arabica	92:109	Salicornia arabica	92:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	4	25	theme	layer	690:694	arg1	TLC					712:714	TLC	712:714	TLC	712:714	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	25	theme	layer	690:694	arg1	chromatography					696:709	Thin layer chromatography	685:709	Thin layer chromatography (TLC) analysis	685:724	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	26	theme	Thin	685:688	arg1	TLC					712:714	TLC	712:714	TLC	712:714	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	26	theme	Thin	685:688	arg1	chromatography					696:709	Thin layer chromatography	685:709	Thin layer chromatography (TLC) analysis	685:724	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	1	27	theme	Salicornia	133:142	arg1	polysaccharides					159:173	Salicornia arabica (SAPS) polysaccharides	133:173	Salicornia arabica (SAPS) polysaccharides	133:173	A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated.					
30217647	2	28	theme	polysaccharide	211:224	arg1	extract					226:232	The water-soluble polysaccharide extract	193:232	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA)	193:275	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	1	29	theme	arabica	144:150	arg1	polysaccharides					159:173	Salicornia arabica (SAPS) polysaccharides	133:173	Salicornia arabica (SAPS) polysaccharides	133:173	A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated.					
30217647	2	30	theme	water-soluble	197:209	arg1	extract					226:232	The water-soluble polysaccharide extract	193:232	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA)	193:275	The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%).					
30217647	0	31	dep	in	9:10	arg1	vitro					12:16	vitro	12:16	vitro	12:16	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	4	32	theme	gaz	616:618	arg1	chromatography					620:633	gaz chromatography	616:633	gaz chromatography	616:633	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	32	theme	gaz	616:618	arg1	analysis					717:724	Thin layer chromatography (TLC) analysis	685:724	Thin layer chromatography (TLC) analysis	685:724	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	4	32	theme	gaz	616:618	arg1	Fourier					636:642	Fourier	636:642	Fourier Transform Infrared Spectrometer (FT-IR)	636:682	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	3	33	theme	Glucopyranose	476:488	arg1	galactose					490:498	Glucopyranose galactose	476:498	Glucopyranose galactose	476:498	The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose.					
30217647	0	34	theme	polysaccharides	62:76	arg1	capacities					48:57	in vitro antioxidant and antimicrobial capacities	9:57	in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica	9:109	Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica.					
30217647	4	35	dep	Fourier	636:642	arg1	Transform					644:652	Transform	644:652	Transform Infrared Spectrometer	644:674	The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis.					
30217647	1	36	theme	SAPS	153:156	arg1	polysaccharides					159:173	Salicornia arabica (SAPS) polysaccharides	133:173	Salicornia arabica (SAPS) polysaccharides	133:173	A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated.					
31220491	3	0	theme	polysaccharides	309:323	arg1	polysaccharides					309:323	polysaccharides	309:323	polysaccharides	309:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	3	0	theme	polysaccharides	309:323	arg1	amount					299:304	a large amount	291:304	a large amount of polysaccharides	291:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	4	1	theme	papain	473:478	arg1	digestion					480:488	papain digestion	473:488	papain digestion	473:488	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	3	2	theme	large	293:297	arg1	polysaccharides					309:323	polysaccharides	309:323	polysaccharides	309:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	3	2	theme	large	293:297	arg1	amount					299:304	a large amount	291:304	a large amount of polysaccharides	291:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	4	3	theme	1D	554:555	arg1	NMR					564:566	1D and 2D NMR	554:566	1D and 2D NMR	554:566	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	0	4	theme	watermelon	82:91	arg1	rinds					93:97	watermelon rinds	82:97	watermelon rinds	82:97	Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.					
31220491	8	5	theme	laryngeal	1319:1327	arg1	cells					1345:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	7	6	attach	linked	1194:1199	arg2	rhamnose					1158:1165	rhamnose	1158:1165	rhamnose linked in α 1 → 4	1158:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	6	attach	linked	1194:1199	arg2	1 → 3					1151:1155	α 1 → 3	1149:1155	α 1 → 3	1149:1155	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	6	attach	linked	1194:1199	arg3	1 → 6					1206:1210	β 1 → 6	1204:1210	β 1 → 6	1204:1210	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	6	attach	linked	1194:1199	arg3	branches					1226:1233	galactose branches	1216:1233	galactose branches	1216:1233	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	6	attach	linked	1194:1199	arg2	mannose					1186:1192	mannose	1186:1192	mannose	1186:1192	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	5	7	theme	extracted	699:707	arg1	polysaccharide					709:722	the extracted polysaccharide	695:722	the extracted polysaccharide	695:722	The monosaccharide composition revealed that the extracted polysaccharide was composed of galactose (38.26%), arabinose (26.12%), rhamnose (17.86%), mannose (9.94%), xylose (5.10%) and glucose (2.70%) with a percentage of uronic acid of 45%.					
31220491	8	8	theme	human	1313:1317	arg1	cells					1345:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	4	9	theme	2D	561:562	arg1	NMR					564:566	1D and 2D NMR	554:566	1D and 2D NMR	554:566	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	3	10	theme	important	360:368	arg1	role					370:373	an important role	357:373	an important role	357:373	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	8	11	theme	Hep-2	1339:1343	arg1	cells					1345:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	7	12	theme	β	1245:1245	arg1	1 → 3					1247:1251	β 1 → 3	1245:1251	β 1 → 3	1245:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	8	13	theme	carcinoma	1329:1337	arg1	cells					1345:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	human laryngeal carcinoma Hep-2 cells	1313:1349	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	7	14	theme	α	1149:1149	arg1	1 → 3					1151:1155	α 1 → 3	1149:1155	α 1 → 3	1149:1155	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	14	theme	α	1149:1149	arg1	rhamnose					1158:1165	rhamnose	1158:1165	rhamnose linked in α 1 → 4	1158:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	14	theme	α	1149:1149	arg1	mannose					1186:1192	mannose	1186:1192	mannose	1186:1192	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	6	15	theme	NMR	917:919	arg1	analysis					921:928	NMR analysis	917:928	NMR analysis	917:928	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	0	16	theme	Partial	0:6	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.					
31220491	7	17	attach	linked	1235:1240	arg3	1 → 3					1247:1251	β 1 → 3	1245:1251	β 1 → 3	1245:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	17	attach	linked	1235:1240	arg2	1 → 6					1206:1210	β 1 → 6	1204:1210	β 1 → 6	1204:1210	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	18	dep	-β-D-Galp-	1079:1088	arg1	1→					1090:1091	1→	1090:1091	1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3	1090:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	18	dep	-β-D-Galp-	1079:1088	arg1	→6					1076:1077	→6	1076:1077	→6	1076:1077	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	4	19	from	locations	614:622	arg1	backbone					640:647	backbone	640:647	backbone	640:647	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	4	19	from	locations	614:622	arg1	branches					627:634	branches	627:634	branches	627:634	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	6	20	theme	CPG	909:911	arg1	analysis					921:928	NMR analysis	917:928	NMR analysis	917:928	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	6	20	theme	CPG	909:911	arg1	combination					894:904	A combination	892:904	A combination of CPG	892:911	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	3	21	theme	health	382:387	arg1	benefits					389:396	the health benefits	378:396	the health benefits	378:396	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	8	22	theme	dose-and	1356:1363	arg1	manner					1380:1385	a dose-and time-dependant manner	1354:1385	a dose-and time-dependant manner	1354:1385	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	5	23	theme	%	889:889	arg1	acid					879:882	uronic acid	872:882	uronic acid of 45%	872:889	The monosaccharide composition revealed that the extracted polysaccharide was composed of galactose (38.26%), arabinose (26.12%), rhamnose (17.86%), mannose (9.94%), xylose (5.10%) and glucose (2.70%) with a percentage of uronic acid of 45%.					
31220491	7	24	attach	linked	1139:1144	arg3	rhamnose					1158:1165	rhamnose	1158:1165	rhamnose linked in α 1 → 4	1158:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	24	attach	linked	1139:1144	arg3	mannose					1186:1192	mannose	1186:1192	mannose	1186:1192	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	24	attach	linked	1139:1144	arg2	arabinose					1129:1137	arabinose	1129:1137	arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3	1129:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	24	attach	linked	1139:1144	arg3	1 → 3					1151:1155	α 1 → 3	1149:1155	α 1 → 3	1149:1155	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	0	25	theme	antitumor	29:37	arg1	activity					39:46	antitumor activity	29:46	antitumor activity	29:46	Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.					
31220491	7	26	theme	galactose	1216:1224	arg1	branches					1226:1233	galactose branches	1216:1233	galactose branches	1216:1233	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	27	theme	β	1204:1204	arg1	1 → 6					1206:1210	β 1 → 6	1204:1210	β 1 → 6	1204:1210	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	6	28	theme	type	1000:1003	arg1	rhamnogalacturonan					1007:1024	type I rhamnogalacturonan	1000:1024	type I rhamnogalacturonan	1000:1024	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	1	29	theme	health-beneficial	105:121	arg1	watermelon					130:139	watermelon	130:139	watermelon	130:139	As a health-beneficial fruit, watermelon is widely consumed by people around the world.					
31220491	1	29	theme	health-beneficial	105:121	arg1	fruit					123:127	a health-beneficial fruit	103:127	a health-beneficial fruit	103:127	As a health-beneficial fruit, watermelon is widely consumed by people around the world.					
31220491	3	30	contain	contains	282:289	arg1	watermelon					271:280	watermelon	271:280	watermelon	271:280	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	3	30	contain	contains	282:289	arg2	polysaccharides					309:323	polysaccharides	309:323	polysaccharides	309:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	3	30	contain	contains	282:289	arg2	amount					299:304	a large amount	291:304	a large amount of polysaccharides	291:323	As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits.					
31220491	4	31	theme	glycosidic	587:596	arg1	linkages					598:605	the glycosidic linkages	583:605	the glycosidic linkages	583:605	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	4	31	theme	glycosidic	587:596	arg1	locations					614:622	their locations	608:622	their locations in branches and backbone	608:647	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	8	32	theme	time-dependant	1365:1378	arg1	manner					1380:1385	a dose-and time-dependant manner	1354:1385	a dose-and time-dependant manner	1354:1385	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	4	33	from	rinds	444:448	arg1	polysaccharide					413:426	polysaccharide	413:426	polysaccharide from watermelon rinds (PWR)	413:454	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	4	34	theme	watermelon	433:442	arg1	rinds					444:448	watermelon rinds	433:448	watermelon rinds (PWR)	433:454	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	4	34	theme	watermelon	433:442	arg1	PWR					451:453	PWR	451:453	PWR	451:453	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	5	35	theme	uronic	872:877	arg1	acid					879:882	uronic acid	872:882	uronic acid of 45%	872:889	The monosaccharide composition revealed that the extracted polysaccharide was composed of galactose (38.26%), arabinose (26.12%), rhamnose (17.86%), mannose (9.94%), xylose (5.10%) and glucose (2.70%) with a percentage of uronic acid of 45%.					
31220491	2	36	theme	health	228:233	arg1	benefits					235:242	the health benefits	224:242	the health benefits	224:242	However, components responsible for the health benefits are not yet determined.					
31220491	7	37	theme	arabinose	1129:1137	arg1	branching					1116:1124	short branching	1110:1124	short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3	1110:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	0	38	theme	polysaccharide	53:66	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.					
31220491	0	38	theme	polysaccharide	53:66	arg1	activity					39:46	antitumor activity	29:46	antitumor activity	29:46	Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.					
31220491	7	39	theme	short	1110:1114	arg1	branching					1116:1124	short branching	1110:1124	short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3	1110:1251	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	4	40	dep	digestion	480:488	arg1	characterized					504:516	characterized	504:516	characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone	504:647	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	4	40	dep	digestion	480:488	arg1	purified					491:498	purified	491:498	purified	491:498	In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone.					
31220491	2	41	theme	responsible	208:218	arg1	components					197:206	components	197:206	components responsible for the health benefits	197:242	However, components responsible for the health benefits are not yet determined.					
31220491	7	42	theme	α	1177:1177	arg1	1 → 4					1179:1183	α 1 → 4	1177:1183	α 1 → 4	1177:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	5	43	theme	acid	879:882	arg1	percentage					858:867	a percentage	856:867	a percentage of uronic acid of 45%	856:889	The monosaccharide composition revealed that the extracted polysaccharide was composed of galactose (38.26%), arabinose (26.12%), rhamnose (17.86%), mannose (9.94%), xylose (5.10%) and glucose (2.70%) with a percentage of uronic acid of 45%.					
31220491	5	44	theme	monosaccharide	654:667	arg1	composition					669:679	The monosaccharide composition	650:679	The monosaccharide composition	650:679	The monosaccharide composition revealed that the extracted polysaccharide was composed of galactose (38.26%), arabinose (26.12%), rhamnose (17.86%), mannose (9.94%), xylose (5.10%) and glucose (2.70%) with a percentage of uronic acid of 45%.					
31220491	8	45	theme	cytotoxicity	1289:1300	arg1	ability					1302:1308	obvious cytotoxicity ability	1281:1308	obvious cytotoxicity ability	1281:1308	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	6	46	theme	extracted	946:954	arg1	polysaccharide					956:969	the extracted polysaccharide	942:969	the extracted polysaccharide	942:969	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	6	46	theme	extracted	946:954	arg1	arabinogalactan					974:988	arabinogalactan	974:988	arabinogalactan linked to type I rhamnogalacturonan	974:1024	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	8	47	theme	obvious	1281:1287	arg1	ability					1302:1308	obvious cytotoxicity ability	1281:1308	obvious cytotoxicity ability	1281:1308	Furthermore, PWR exhibited obvious cytotoxicity ability to human laryngeal carcinoma Hep-2 cells in a dose-and time-dependant manner.					
31220491	6	48	attach	linked	990:995	arg2	arabinogalactan					974:988	arabinogalactan	974:988	arabinogalactan linked to type I rhamnogalacturonan	974:1024	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	6	48	attach	linked	990:995	arg2	polysaccharide					956:969	the extracted polysaccharide	942:969	the extracted polysaccharide	942:969	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	6	48	attach	linked	990:995	arg1	rhamnogalacturonan					1007:1024	type I rhamnogalacturonan	1000:1024	type I rhamnogalacturonan	1000:1024	A combination of CPG and NMR analysis showed that the extracted polysaccharide is arabinogalactan linked to type I rhamnogalacturonan.					
31220491	7	49	attach	linked	1167:1172	arg2	rhamnose					1158:1165	rhamnose	1158:1165	rhamnose linked in α 1 → 4	1158:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	49	attach	linked	1167:1172	arg2	1 → 3					1151:1155	α 1 → 3	1149:1155	α 1 → 3	1149:1155	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
31220491	7	49	attach	linked	1167:1172	arg3	1 → 4					1179:1183	α 1 → 4	1177:1183	α 1 → 4	1177:1183	we notice that the arabinogalactan was formed by →6)-β-D-Galp-(1→ as backbone with short branching of arabinose linked in α 1 → 3, rhamnose linked in α 1 → 4, mannose linked in β 1 → 6 and galactose branches linked in β 1 → 3.					
29415413	0	0	theme	polysaccharides	77:91	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	0	0	theme	polysaccharides	77:91	arg1	activity					43:50	anticancer activity	32:50	anticancer activity	32:50	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	4	1	contain	contains	668:675	arg2	polysaccharide					687:700	a neutral polysaccharide	677:700	a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content	677:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	4	1	contain	contains	668:675	arg1	composition					624:634	The chemical composition	611:634	The chemical composition of the extracted polysaccharide	611:666	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	8	2	theme	breast	1319:1324	arg1	cancer					1326:1331	breast cancer	1319:1331	breast cancer	1319:1331	Finally, polysaccharides increased the activity of caspase 3/9, thus leads to apoptosis of breast cancer.					
29415413	4	3	with	polysaccharide	687:700	arg1	content					772:778	low protein, phenol and flavonoid content	738:778	content	772:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	4	3	with	polysaccharide	687:700	arg1	content					726:732	a high total sugar content	707:732	a high total sugar content	707:732	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	9	4	from	wightii	1368:1374	arg1	source					1391:1396	a new source	1385:1396	a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs	1385:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	9	4	from	wightii	1368:1374	arg1	polysaccharides					1344:1358	polysaccharides	1344:1358	polysaccharides from S. wightii	1344:1374	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	1	5	theme	study	164:168	arg1	purpose					148:154	The purpose	144:154	The purpose of this study	144:168	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	5	6	dep	spectra	859:865	arg1	shows					867:871	shows	867:871	shows the presence of β-galactose signals	867:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	3	7	theme	%	539:539	arg1	yield					525:529	the yield	521:529	the yield of 21.48%	521:539	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	9	8	theme	natural	1401:1407	arg1	agent					1420:1424	natural anticancer agent	1401:1424	natural anticancer agent against breast cancer	1401:1446	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	9	9	theme	manufacturing	1476:1488	arg1	supplements					1490:1500	the manufacturing supplements	1472:1500	the manufacturing supplements	1472:1500	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	8	10	theme	cancer	1326:1331	arg1	apoptosis					1306:1314	apoptosis	1306:1314	apoptosis of breast cancer	1306:1331	Finally, polysaccharides increased the activity of caspase 3/9, thus leads to apoptosis of breast cancer.					
29415413	9	11	theme	anticancer	1409:1418	arg1	agent					1420:1424	natural anticancer agent	1401:1424	natural anticancer agent against breast cancer	1401:1446	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	5	12	theme	galactofuranose	821:835	arg1	presence					809:816	the presence	805:816	the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals	805:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	4	13	theme	polysaccharide	653:666	arg1	composition					624:634	The chemical composition	611:634	The chemical composition of the extracted polysaccharide	611:666	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	9	14	theme	potential	1453:1461	arg1	value					1463:1467	potential value	1453:1467	potential value in the manufacturing supplements and drugs	1453:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	4	15	theme	protein	742:748	arg1	content					772:778	low protein, phenol and flavonoid content	738:778	content	772:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	5	16	theme	β-galactose	889:899	arg1	signals					901:907	β-galactose signals	889:907	β-galactose signals	889:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	4	17	theme	chemical	615:622	arg1	composition					624:634	The chemical composition	611:634	The chemical composition of the extracted polysaccharide	611:666	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	7	18	theme	breast	1127:1132	arg1	cells					1141:1145	the breast cancer cells	1123:1145	the breast cancer cells	1123:1145	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	4	19	theme	flavonoid	762:770	arg1	content					772:778	low protein, phenol and flavonoid content	738:778	content	772:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	7	20	theme	mitochondrial	1186:1198	arg1	membrane					1200:1207	mitochondrial membrane	1186:1207	mitochondrial membrane	1186:1207	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	4	21	theme	low	738:740	arg1	content					772:778	low protein, phenol and flavonoid content	738:778	content	772:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	6	22	theme	dose-dependent	1050:1063	arg1	manner					1065:1070	a dose-dependent manner	1048:1070	a dose-dependent manner	1048:1070	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	7	23	theme	ROS	1161:1163	arg1	generation					1165:1174	ROS generation	1161:1174	ROS generation	1161:1174	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	6	24	dep	cells	1017:1021	arg1	MDA-MB-231					1033:1042	MDA-MB-231	1033:1042	MDA-MB-231	1033:1042	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	24	dep	cells	1017:1021	arg1	MCF7					1024:1027	MCF7	1024:1027	MCF7	1024:1027	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	24	dep	cells	1017:1021	arg1	cells					1017:1021	breast cancer cells	1003:1021	breast cancer cells (MCF7 and MDA-MB-231)	1003:1043	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	4	25	theme	high	709:712	arg1	content					726:732	a high total sugar content	707:732	a high total sugar content	707:732	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	5	26	theme	arabinose	841:849	arg1	presence					809:816	the presence	805:816	the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals	805:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	0	27	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	9	28	theme	new	1387:1389	arg1	polysaccharides					1344:1358	polysaccharides	1344:1358	polysaccharides from S. wightii	1344:1374	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	9	28	theme	new	1387:1389	arg1	source					1391:1396	a new source	1385:1396	a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs	1385:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	0	29	theme	anticancer	32:41	arg1	activity					43:50	anticancer activity	32:50	anticancer activity	32:50	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	1	30	theme	human	278:282	arg1	cells					298:302	human breast cancer cells	278:302	human breast cancer cells	278:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	9	31	theme	agent	1420:1424	arg1	polysaccharides					1344:1358	polysaccharides	1344:1358	polysaccharides from S. wightii	1344:1374	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	9	31	theme	agent	1420:1424	arg1	source					1391:1396	a new source	1385:1396	a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs	1385:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	4	32	theme	sugar	720:724	arg1	content					726:732	a high total sugar content	707:732	a high total sugar content	707:732	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	1	33	theme	anticancer	193:202	arg1	activity					204:211	the anticancer activity	189:211	the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells	189:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	34	theme	breast	284:289	arg1	cells					298:302	human breast cancer cells	278:302	human breast cancer cells	278:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	6	35	theme	cancer	1010:1015	arg1	MDA-MB-231					1033:1042	MDA-MB-231	1033:1042	MDA-MB-231	1033:1042	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	35	theme	cancer	1010:1015	arg1	MCF7					1024:1027	MCF7	1024:1027	MCF7	1024:1027	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	35	theme	cancer	1010:1015	arg1	cells					1017:1021	breast cancer cells	1003:1021	breast cancer cells (MCF7 and MDA-MB-231)	1003:1043	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	2	36	dep	fractions	335:343	arg1	fractions					335:343	two polysaccharide fractions	316:343	two polysaccharide fractions (SWP1 and SWP2)	316:359	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	2	36	dep	fractions	335:343	arg1	SWP2					355:358	SWP2	355:358	SWP2	355:358	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	2	36	dep	fractions	335:343	arg1	SWP1					346:349	SWP1	346:349	SWP1	346:349	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	7	37	from	apoptosis	1110:1118	arg1	cells					1141:1145	the breast cancer cells	1123:1145	the breast cancer cells	1123:1145	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	2	38	theme	crude	397:401	arg1	polysaccharides					403:417	the crude polysaccharides	393:417	the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography	393:482	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	7	39	theme	cancer	1134:1139	arg1	cells					1141:1145	the breast cancer cells	1123:1145	the breast cancer cells	1123:1145	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	1	40	theme	cancer	291:296	arg1	cells					298:302	human breast cancer cells	278:302	human breast cancer cells	278:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	41	from	wightii	261:267	arg1	activity					204:211	the anticancer activity	189:211	the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells	189:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	41	from	wightii	261:267	arg1	polysaccharides					216:230	polysaccharides	216:230	polysaccharides from brown seaweed Sargassum wightii (SWP)	216:273	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	8	42	theme	caspase	1279:1285	arg1	3/9					1287:1289	caspase 3/9	1279:1289	caspase 3/9	1279:1289	Finally, polysaccharides increased the activity of caspase 3/9, thus leads to apoptosis of breast cancer.					
29415413	2	43	theme	column	462:467	arg1	chromatography					469:482	DEAE-52 cellulose and Sephadex G-100 column chromatography	425:482	chromatography	469:482	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	2	44	theme	DEAE-52	425:431	arg1	cellulose					433:441	DEAE-52 cellulose and Sephadex G-100 column chromatography	425:482	cellulose	433:441	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	9	45	from	value	1463:1467	arg1	supplements					1490:1500	the manufacturing supplements	1472:1500	the manufacturing supplements	1472:1500	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	9	45	from	value	1463:1467	arg1	drugs					1506:1510	drugs	1506:1510	drugs	1506:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	1	46	theme	polysaccharides	216:230	arg1	activity					204:211	the anticancer activity	189:211	the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells	189:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	2	47	theme	G-100	456:460	arg1	chromatography					469:482	DEAE-52 cellulose and Sephadex G-100 column chromatography	425:482	chromatography	469:482	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	5	48	theme	NMR	855:857	arg1	spectra					859:865	NMR spectra	855:865	NMR spectra shows the presence of β-galactose signals	855:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	3	49	theme	13C	602:604	arg1	NMR					606:608	13C NMR	602:608	13C NMR	602:608	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	3	49	theme	13C	602:604	arg1	analysis					574:581	chemical analysis	565:581	chemical analysis	565:581	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	5	50	theme	GC-MS	781:785	arg1	analysis					787:794	GC-MS analysis	781:794	GC-MS analysis	781:794	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	4	51	theme	neutral	679:685	arg1	polysaccharide					687:700	a neutral polysaccharide	677:700	a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content	677:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	2	52	theme	Sephadex	447:454	arg1	chromatography					469:482	DEAE-52 cellulose and Sephadex G-100 column chromatography	425:482	chromatography	469:482	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	6	53	theme	Anticancer	910:919	arg1	activity					921:928	Anticancer activity	910:928	Anticancer activity	910:928	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	3	54	theme	chemical	565:572	arg1	analysis					574:581	chemical analysis	565:581	chemical analysis	565:581	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	3	54	theme	chemical	565:572	arg1	NMR					606:608	13C NMR	602:608	13C NMR	602:608	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	3	54	theme	chemical	565:572	arg1	GC-MS					584:588	GC-MS	584:588	GC-MS	584:588	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	3	54	theme	chemical	565:572	arg1	NMR					594:596	1H NMR	591:596	1H NMR	591:596	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	9	55	with	source	1391:1396	arg1	value					1463:1467	potential value	1453:1467	potential value in the manufacturing supplements and drugs	1453:1510	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	8	56	theme	3/9	1287:1289	arg1	activity					1267:1274	the activity	1263:1274	the activity of caspase 3/9	1263:1289	Finally, polysaccharides increased the activity of caspase 3/9, thus leads to apoptosis of breast cancer.					
29415413	6	57	theme	cells	1017:1021	arg1	proliferation					986:998	the proliferation	982:998	the proliferation of breast cancer cells (MCF7 and MDA-MB-231)	982:1043	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	4	58	theme	phenol	751:756	arg1	content					772:778	low protein, phenol and flavonoid content	738:778	content	772:778	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	3	59	theme	1H	591:592	arg1	analysis					574:581	chemical analysis	565:581	chemical analysis	565:581	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	3	59	theme	1H	591:592	arg1	NMR					594:596	1H NMR	591:596	1H NMR	591:596	As a result, SWP1 was obtained with the yield of 21.48% was characterized using chemical analysis, GC-MS, 1H NMR and 13C NMR.					
29415413	1	60	from	activity	204:211	arg1	SWP					270:272	SWP	270:272	SWP	270:272	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	60	from	activity	204:211	arg1	cells					298:302	human breast cancer cells	278:302	human breast cancer cells	278:302	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	60	from	activity	204:211	arg1	wightii					261:267	brown seaweed Sargassum wightii	237:267	brown seaweed Sargassum wightii (SWP)	237:273	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	2	61	theme	polysaccharide	320:333	arg1	fractions					335:343	two polysaccharide fractions	316:343	two polysaccharide fractions (SWP1 and SWP2)	316:359	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	2	61	theme	polysaccharide	320:333	arg1	SWP2					355:358	SWP2	355:358	SWP2	355:358	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	2	61	theme	polysaccharide	320:333	arg1	SWP1					346:349	SWP1	346:349	SWP1	346:349	Initially, two polysaccharide fractions (SWP1 and SWP2) were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29415413	7	62	dep	membrane	1200:1207	arg1	damage					1220:1225	damage	1220:1225	damage	1220:1225	Further, polysaccharides induced the apoptosis in the breast cancer cells by increasing ROS generation, cleaving mitochondrial membrane and nuclei damage.					
29415413	4	63	theme	total	714:718	arg1	content					726:732	a high total sugar content	707:732	a high total sugar content	707:732	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	5	64	theme	signals	901:907	arg1	presence					877:884	the presence	873:884	the presence of β-galactose signals	873:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	1	65	theme	brown	237:241	arg1	SWP					270:272	SWP	270:272	SWP	270:272	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	65	theme	brown	237:241	arg1	wightii					261:267	brown seaweed Sargassum wightii	237:267	brown seaweed Sargassum wightii (SWP)	237:273	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	0	66	dep	characterization	11:26	arg1	MDA-MB-231					62:71	MDA-MB-231	62:71	MDA-MB-231	62:71	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	0	66	dep	characterization	11:26	arg1	MCF7					53:56	MCF7	53:56	MCF7	53:56	Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.					
29415413	4	67	theme	extracted	643:651	arg1	polysaccharide					653:666	the extracted polysaccharide	639:666	the extracted polysaccharide	639:666	The chemical composition of the extracted polysaccharide contains a neutral polysaccharide with a high total sugar content and low protein, phenol and flavonoid content.					
29415413	1	68	theme	seaweed	243:249	arg1	SWP					270:272	SWP	270:272	SWP	270:272	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	68	theme	seaweed	243:249	arg1	wightii					261:267	brown seaweed Sargassum wightii	237:267	brown seaweed Sargassum wightii (SWP)	237:273	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	9	69	theme	breast	1434:1439	arg1	cancer					1441:1446	breast cancer	1434:1446	breast cancer	1434:1446	Together, polysaccharides from S. wightii could be a new source of natural anticancer agent against breast cancer with potential value in the manufacturing supplements and drugs.					
29415413	6	70	theme	breast	1003:1008	arg1	MDA-MB-231					1033:1042	MDA-MB-231	1033:1042	MDA-MB-231	1033:1042	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	70	theme	breast	1003:1008	arg1	MCF7					1024:1027	MCF7	1024:1027	MCF7	1024:1027	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	6	70	theme	breast	1003:1008	arg1	cells					1017:1021	breast cancer cells	1003:1021	breast cancer cells (MCF7 and MDA-MB-231)	1003:1043	Anticancer activity shows that the polysaccharides significantly reduce the proliferation of breast cancer cells (MCF7 and MDA-MB-231) in a dose-dependent manner.					
29415413	5	71	theme	spectra	859:865	arg1	presence					809:816	the presence	805:816	the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals	805:907	GC-MS analysis revealed the presence of galactofuranose and arabinose and NMR spectra shows the presence of β-galactose signals.					
29415413	1	72	theme	Sargassum	251:259	arg1	SWP					270:272	SWP	270:272	SWP	270:272	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
29415413	1	72	theme	Sargassum	251:259	arg1	wightii					261:267	brown seaweed Sargassum wightii	237:267	brown seaweed Sargassum wightii (SWP)	237:273	The purpose of this study was to investigate the anticancer activity of polysaccharides from brown seaweed Sargassum wightii (SWP) on human breast cancer cells.					
30439439	0	0	theme	L.	85:86	arg1	Polysaccharides					88:102	Arctium lappa L. Polysaccharides	71:102	Arctium lappa L. Polysaccharides	71:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	3	1	theme	molecular	707:715	arg1	weight					717:722	a molecular weight	705:722	a molecular weight of 218, 178, and 60 kDa, respectively	705:760	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	0	2	theme	lappa	79:83	arg1	Polysaccharides					88:102	Arctium lappa L. Polysaccharides	71:102	Arctium lappa L. Polysaccharides	71:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	3	theme	ALP80-1	569:575	arg1	fractions					534:542	fractions	534:542	fractions of ALP40-1, ALP60-1, and ALP80-1	534:575	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	4	theme	optimal	354:360	arg1	conditions					362:371	The modified optimal conditions	341:371	The modified optimal conditions	341:371	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	5	5	theme	potential	1069:1077	arg1	ALP60-1					1056:1062	ALP60-1	1056:1062	ALP60-1	1056:1062	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	5	5	theme	potential	1069:1077	arg1	antioxidant					1093:1103	a potential novel natural antioxidant	1067:1103	a potential novel natural antioxidant	1067:1103	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	0	6	from	Extraction	0:9	arg1	roots					62:66	roots	62:66	roots of Arctium lappa L. Polysaccharides	62:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	7	theme	response	167:174	arg1	methodology					184:194	response surface methodology	167:194	response surface methodology with ultrasonication	167:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	5	8	theme	effective	1014:1022	arg1	extraction					993:1002	ultrasonic-assisted extraction	973:1002	ultrasonic-assisted extraction	973:1002	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	5	8	theme	effective	1014:1022	arg1	method					1024:1029	a very effective method	1007:1029	a very effective method for extracting ALPs	1007:1049	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	0	9	theme	Polysaccharides	88:102	arg1	roots					62:66	roots	62:66	roots of Arctium lappa L. Polysaccharides	62:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	10	from	roots	62:66	arg1	polysaccharides					41:55	polysaccharides	41:55	polysaccharides from roots of Arctium lappa L. Polysaccharides	41:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	10	from	roots	62:66	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	10	from	roots	62:66	arg1	activities					27:36	antioxidant activities	15:36	antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides	15:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	11	theme	extraction	457:466	arg1	time					468:471	extraction time	457:471	extraction time of 83 min	457:481	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	12	theme	activity	620:627	arg1	analyses					629:636	chemical and antioxidant activity analyses	595:636	chemical and antioxidant activity analyses	595:636	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	13	theme	extraction	488:497	arg1	temperature					499:509	extraction temperature	488:509	extraction temperature	488:509	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	5	14	theme	novel	1079:1083	arg1	ALP60-1					1056:1062	ALP60-1	1056:1062	ALP60-1	1056:1062	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	5	14	theme	novel	1079:1083	arg1	antioxidant					1093:1103	a potential novel natural antioxidant	1067:1103	a potential novel natural antioxidant	1067:1103	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	2	15	theme	antioxidant	608:618	arg1	analyses					629:636	chemical and antioxidant activity analyses	595:636	chemical and antioxidant activity analyses	595:636	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	4	16	theme	superoxide	921:930	arg1	radicals					932:939	superoxide radicals	921:939	superoxide radicals	921:939	ALP60-1 exhibited strong scavenging activities on 1,1‑diphenyl‑2‑picryhydrazyl, hydroxyl, and superoxide radicals.					
30439439	5	17	theme	natural	1085:1091	arg1	ALP60-1					1056:1062	ALP60-1	1056:1062	ALP60-1	1056:1062	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	5	17	theme	natural	1085:1091	arg1	antioxidant					1093:1103	a potential novel natural antioxidant	1067:1103	a potential novel natural antioxidant	1067:1103	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	0	18	theme	surface	176:182	arg1	methodology					184:194	response surface methodology	167:194	response surface methodology with ultrasonication	167:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	19	dep	were	373:376	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	20	theme	water	390:394	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	21	theme	power	441:445	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	22	theme	time	468:471	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	6	23	theme	in	1149:1150	arg1	experiments					1157:1167	in vivo experiments	1149:1167	in vivo experiments	1149:1167	However, further structure elucidation and in vivo experiments are required.					
30439439	6	24	dep	in	1149:1150	arg1	vivo					1152:1155	vivo	1152:1155	vivo	1152:1155	However, further structure elucidation and in vivo experiments are required.					
30439439	2	25	theme	ultrasonic	430:439	arg1	power					441:445	ultrasonic power	430:445	ultrasonic power of 158 W	430:454	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	1	26	theme	extraction	268:277	arg1	parameters					279:288	extraction parameters	268:288	extraction parameters	268:288	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30439439	2	27	dep	ratio	412:416	arg1	to					396:397	to	396:397	to	396:397	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	0	28	theme	antioxidant	15:25	arg1	activities					27:36	antioxidant activities	15:36	antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides	15:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	6	29	theme	structure	1123:1131	arg1	elucidation					1133:1143	further structure elucidation	1115:1143	further structure elucidation	1115:1143	However, further structure elucidation and in vivo experiments are required.					
30439439	0	30	with	methodology	184:194	arg1	ultrasonication					201:215	ultrasonication	201:215	ultrasonication	201:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	31	theme	polysaccharides	41:55	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	0	31	theme	polysaccharides	41:55	arg1	activities					27:36	antioxidant activities	15:36	antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides	15:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	3	32	theme	218	727:729	arg1	weight					717:722	a molecular weight	705:722	a molecular weight of 218, 178, and 60 kDa, respectively	705:760	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	0	33	theme	Arctium	137:143	arg1	roots					128:132	the roots	124:132	the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication	124:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	1	34	used	used	251:254	arg2	design					240:245	A central composition design	218:245	A central composition design	218:245	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30439439	2	35	theme	material	403:410	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	3	36	theme	60 kDa	741:746	arg1	weight					717:722	a molecular weight	705:722	a molecular weight of 218, 178, and 60 kDa, respectively	705:760	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	2	37	theme	50 °C.	514:519	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	38	theme	raw	399:401	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	39	theme	158 W	450:454	arg1	31 mL/g					421:427	31 mL/g	421:427	31 mL/g	421:427	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	39	theme	158 W	450:454	arg1	power					441:445	ultrasonic power	430:445	ultrasonic power of 158 W	430:454	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	39	theme	158 W	450:454	arg1	temperature					499:509	extraction temperature	488:509	extraction temperature	488:509	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	39	theme	158 W	450:454	arg1	time					468:471	extraction time	457:471	extraction time of 83 min	457:481	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	40	theme	ALP60-1	556:562	arg1	fractions					534:542	fractions	534:542	fractions of ALP40-1, ALP60-1, and ALP80-1	534:575	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	3	41	theme	178	732:734	arg1	weight					717:722	a molecular weight	705:722	a molecular weight of 218, 178, and 60 kDa, respectively	705:760	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	2	42	theme	temperature	499:509	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	43	theme	modified	345:352	arg1	conditions					362:371	The modified optimal conditions	341:371	The modified optimal conditions	341:371	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	1	44	theme	polysaccharide	308:321	arg1	yield					334:338	the polysaccharide extraction yield	304:338	the polysaccharide extraction yield	304:338	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30439439	0	45	theme	lappa	145:149	arg1	L					151:151	lappa L	145:151	Arctium lappa L. (ALPs) using response surface methodology with ultrasonication	137:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	46	theme	chemical	595:602	arg1	analyses					629:636	chemical and antioxidant activity analyses	595:636	chemical and antioxidant activity analyses	595:636	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	3	47	contain	had	701:703	arg2	weight					717:722	a molecular weight	705:722	a molecular weight of 218, 178, and 60 kDa, respectively	705:760	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	3	47	contain	had	701:703	arg1	fractions					691:699	the three fractions	681:699	the three fractions	681:699	Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose.					
30439439	1	48	theme	extraction	323:332	arg1	yield					334:338	the polysaccharide extraction yield	304:338	the polysaccharide extraction yield	304:338	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30439439	2	49	theme	ALP40-1	547:553	arg1	fractions					534:542	fractions	534:542	fractions of ALP40-1, ALP60-1, and ALP80-1	534:575	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	4	50	theme	scavenging	852:861	arg1	activities					863:872	strong scavenging activities	845:872	strong scavenging activities	845:872	ALP60-1 exhibited strong scavenging activities on 1,1‑diphenyl‑2‑picryhydrazyl, hydroxyl, and superoxide radicals.					
30439439	5	51	theme	ultrasonic-assisted	973:991	arg1	extraction					993:1002	ultrasonic-assisted extraction	973:1002	ultrasonic-assisted extraction	973:1002	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	5	51	theme	ultrasonic-assisted	973:991	arg1	method					1024:1029	a very effective method	1007:1029	a very effective method for extracting ALPs	1007:1049	These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant.					
30439439	6	52	theme	further	1115:1121	arg1	elucidation					1133:1143	further structure elucidation	1115:1143	further structure elucidation	1115:1143	However, further structure elucidation and in vivo experiments are required.					
30439439	0	53	theme	Arctium	71:77	arg1	Polysaccharides					88:102	Arctium lappa L. Polysaccharides	71:102	Arctium lappa L. Polysaccharides	71:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	54	dep	50 °C.	514:519	arg1	obtained					582:589	obtained	582:589	were obtained for chemical and antioxidant activity analyses after purification	577:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	0	55	dep	Arctium	137:143	arg1	L					151:151	lappa L	145:151	Arctium lappa L. (ALPs) using response surface methodology with ultrasonication	137:215	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	1	56	theme	central	220:226	arg1	design					240:245	A central composition design	218:245	A central composition design	218:245	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30439439	2	57	theme	83 min	476:481	arg1	31 mL/g					421:427	31 mL/g	421:427	31 mL/g	421:427	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	57	theme	83 min	476:481	arg1	power					441:445	ultrasonic power	430:445	ultrasonic power of 158 W	430:454	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	57	theme	83 min	476:481	arg1	temperature					499:509	extraction temperature	488:509	extraction temperature	488:509	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	2	57	theme	83 min	476:481	arg1	time					468:471	extraction time	457:471	extraction time of 83 min	457:481	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	0	58	from	activities	27:36	arg1	roots					62:66	roots	62:66	roots of Arctium lappa L. Polysaccharides	62:102	Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L. Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication.					
30439439	2	59	theme	31 mL/g	421:427	arg1	ratio					412:416	water to raw material ratio	390:416	water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification	390:655	The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification.					
30439439	4	60	theme	strong	845:850	arg1	activities					863:872	strong scavenging activities	845:872	strong scavenging activities	845:872	ALP60-1 exhibited strong scavenging activities on 1,1‑diphenyl‑2‑picryhydrazyl, hydroxyl, and superoxide radicals.					
30439439	1	61	theme	composition	228:238	arg1	design					240:245	A central composition design	218:245	A central composition design	218:245	A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield.					
30553308	2	0	theme	tight	552:556	arg1	proteins					567:574	tight junction proteins	552:574	tight junction proteins	552:574	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	1	theme	cytokines	799:807	arg1	production					785:794	the production	781:794	the production of cytokines	781:807	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	1	theme	cytokines	799:807	arg1	development					828:838	the functional development	813:838	the functional development of immune cells	813:854	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	5	2	used	used	1115:1118	arg2	agent					1134:1138	functional agent	1123:1138	functional agent	1123:1138	These results indicated that GXG might be used as functional agent to improve host health.					
30553308	5	2	used	used	1115:1118	arg2	GXG					1102:1104	GXG	1102:1104	GXG	1102:1104	These results indicated that GXG might be used as functional agent to improve host health.					
30553308	0	3	theme	intestinal	99:108	arg1	microbiota					110:119	intestinal microbiota	99:119	intestinal microbiota	99:119	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	2	4	theme	mucin-2	676:682	arg1	secretion					663:671	secretion	663:671	secretion	663:671	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	4	theme	mucin-2	676:682	arg1	expression					648:657	expression	648:657	expression	648:657	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	5	theme	intestinal	724:733	arg1	function					757:764	the intestinal immunological barrier function	720:764	the intestinal immunological barrier function	720:764	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	6	theme	homogeneous	178:188	arg1	polysaccharide					213:226	a homogeneous Dendrobium huoshanense polysaccharide	176:226	a homogeneous Dendrobium huoshanense polysaccharide (GXG)	176:232	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	6	theme	homogeneous	178:188	arg1	GXG					229:231	GXG	229:231	GXG	229:231	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	2	7	theme	intestinal	591:600	arg1	function					622:629	the intestinal biochemical barrier function	587:629	the intestinal biochemical barrier function	587:629	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	8	theme	different	292:300	arg1	regions					313:319	different intestinal regions	292:319	different intestinal regions of mice	292:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	9	from	effects	165:171	arg1	function					253:260	mucosal barrier function	237:260	mucosal barrier function	237:260	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	9	from	effects	165:171	arg1	composition					277:287	microbiota composition	266:287	microbiota composition	266:287	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	2	10	theme	cells	850:854	arg1	production					785:794	the production	781:794	the production of cytokines	781:807	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	10	theme	cells	850:854	arg1	development					828:838	the functional development	813:838	the functional development of immune cells	813:854	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	11	theme	intestinal	302:311	arg1	regions					313:319	different intestinal regions	292:319	different intestinal regions of mice	292:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	2	12	theme	physiological	381:393	arg1	status					395:400	the intestinal physiological status	366:400	the intestinal physiological status	366:400	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	13	theme	immune	843:848	arg1	cells					850:854	immune cells	843:854	immune cells	843:854	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	14	theme	Dendrobium	190:199	arg1	polysaccharide					213:226	a homogeneous Dendrobium huoshanense polysaccharide	176:226	a homogeneous Dendrobium huoshanense polysaccharide (GXG)	176:232	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	14	theme	Dendrobium	190:199	arg1	GXG					229:231	GXG	229:231	GXG	229:231	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	2	15	theme	sIgA	701:704	arg1	secretion					663:671	secretion	663:671	secretion	663:671	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	15	theme	sIgA	701:704	arg1	expression					648:657	expression	648:657	expression	648:657	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	16	theme	mucosal	497:503	arg1	structures					505:514	mucosal structures	497:514	mucosal structures	497:514	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	17	theme	huoshanense	201:211	arg1	polysaccharide					213:226	a homogeneous Dendrobium huoshanense polysaccharide	176:226	a homogeneous Dendrobium huoshanense polysaccharide (GXG)	176:232	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	17	theme	huoshanense	201:211	arg1	GXG					229:231	GXG	229:231	GXG	229:231	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	0	18	theme	huoshanense	11:21	arg1	regionally					38:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	2	19	dep	expression	648:657	arg1	the					644:646	the	644:646	the	644:646	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	20	theme	polysaccharide	213:226	arg1	effects					165:171	the effects	161:171	the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice	161:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	0	21	theme	Dendrobium	0:9	arg1	regionally					38:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	2	22	theme	β-defensins	685:695	arg1	secretion					663:671	secretion	663:671	secretion	663:671	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	22	theme	β-defensins	685:695	arg1	expression					648:657	expression	648:657	expression	648:657	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	23	theme	barrier	749:755	arg1	function					757:764	the intestinal immunological barrier function	720:764	the intestinal immunological barrier function	720:764	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	24	theme	barrier	614:620	arg1	function					622:629	the intestinal biochemical barrier function	587:629	the intestinal biochemical barrier function	587:629	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	25	theme	immunological	735:747	arg1	function					757:764	the intestinal immunological barrier function	720:764	the intestinal immunological barrier function	720:764	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	0	26	theme	polysaccharide	23:36	arg1	regionally					38:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally	0:47	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	1	27	theme	mice	324:327	arg1	regions					313:319	different intestinal regions	292:319	different intestinal regions of mice	292:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	0	28	from	microbiota	110:119	arg1	mice					124:127	mice	124:127	mice	124:127	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	2	29	theme	barrier	466:472	arg1	function					474:481	the intestinal physical barrier function	442:481	the intestinal physical barrier function	442:481	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	0	30	theme	intestinal	59:68	arg1	function					86:93	intestinal mucosal barrier function	59:93	intestinal mucosal barrier function	59:93	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	1	31	from	composition	277:287	arg1	regions					313:319	different intestinal regions	292:319	different intestinal regions of mice	292:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	4	32	theme	peripheral	1021:1030	arg1	blood					1032:1036	peripheral blood	1021:1036	peripheral blood	1021:1036	In addition, the immune response in spleen and peripheral blood were effectively regulated by GXG.					
30553308	2	33	theme	physical	457:464	arg1	function					474:481	the intestinal physical barrier function	442:481	the intestinal physical barrier function	442:481	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	34	theme	biochemical	602:612	arg1	function					622:629	the intestinal biochemical barrier function	587:629	the intestinal biochemical barrier function	587:629	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	35	theme	mucosal	237:243	arg1	function					253:260	mucosal barrier function	237:260	mucosal barrier function	237:260	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	0	36	theme	barrier	78:84	arg1	function					86:93	intestinal mucosal barrier function	59:93	intestinal mucosal barrier function	59:93	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	4	37	theme	immune	991:996	arg1	response					998:1005	the immune response	987:1005	the immune response in spleen and peripheral blood	987:1036	In addition, the immune response in spleen and peripheral blood were effectively regulated by GXG.					
30553308	0	38	from	function	86:93	arg1	mice					124:127	mice	124:127	mice	124:127	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	1	39	theme	present	134:140	arg1	study					142:146	The present study	130:146	The present study	130:146	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	0	40	theme	mucosal	70:76	arg1	function					86:93	intestinal mucosal barrier function	59:93	intestinal mucosal barrier function	59:93	Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.					
30553308	3	41	dep	composition	909:919	arg1	the					905:907	the	905:907	the	905:907	Simultaneously, GXG could differentially impact the composition and metabolism of microbiota along intestinal tract.					
30553308	5	42	theme	functional	1123:1132	arg1	GXG					1102:1104	GXG	1102:1104	GXG	1102:1104	These results indicated that GXG might be used as functional agent to improve host health.					
30553308	5	42	theme	functional	1123:1132	arg1	agent					1134:1138	functional agent	1123:1138	functional agent	1123:1138	These results indicated that GXG might be used as functional agent to improve host health.					
30553308	2	43	theme	intestinal	370:379	arg1	status					395:400	the intestinal physiological status	366:400	the intestinal physiological status	366:400	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	3	44	theme	intestinal	956:965	arg1	tract					967:971	intestinal tract	956:971	intestinal tract	956:971	Simultaneously, GXG could differentially impact the composition and metabolism of microbiota along intestinal tract.					
30553308	5	45	theme	host	1151:1154	arg1	health					1156:1161	host health	1151:1161	host health	1151:1161	These results indicated that GXG might be used as functional agent to improve host health.					
30553308	4	46	from	response	998:1005	arg1	spleen					1010:1015	spleen	1010:1015	spleen	1010:1015	In addition, the immune response in spleen and peripheral blood were effectively regulated by GXG.					
30553308	4	46	from	response	998:1005	arg1	blood					1032:1036	peripheral blood	1021:1036	peripheral blood	1021:1036	In addition, the immune response in spleen and peripheral blood were effectively regulated by GXG.					
30553308	1	47	theme	barrier	245:251	arg1	function					253:260	mucosal barrier function	237:260	mucosal barrier function	237:260	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	1	48	from	function	253:260	arg1	regions					313:319	different intestinal regions	292:319	different intestinal regions of mice	292:327	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
30553308	2	49	theme	functional	817:826	arg1	development					828:838	the functional development	813:838	the functional development of immune cells	813:854	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	3	50	theme	microbiota	939:948	arg1	metabolism					925:934	metabolism	925:934	metabolism	925:934	Simultaneously, GXG could differentially impact the composition and metabolism of microbiota along intestinal tract.					
30553308	3	50	theme	microbiota	939:948	arg1	composition					909:919	composition	909:919	composition	909:919	Simultaneously, GXG could differentially impact the composition and metabolism of microbiota along intestinal tract.					
30553308	2	51	theme	proteins	567:574	arg1	expression					538:547	the expression	534:547	the expression of tight junction proteins	534:574	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	52	theme	intestinal	446:455	arg1	function					474:481	the intestinal physical barrier function	442:481	the intestinal physical barrier function	442:481	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	53	theme	administrated	410:422	arg1	GXG					424:426	orally administrated GXG	403:426	orally administrated GXG	403:426	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	2	54	theme	junction	558:565	arg1	proteins					567:574	tight junction proteins	552:574	tight junction proteins	552:574	Results exhibited, besides changing the intestinal physiological status, orally administrated GXG could improve the intestinal physical barrier function by modulating mucosal structures and up-regulating the expression of tight junction proteins, reinforce the intestinal biochemical barrier function by elevating the expression and secretion of mucin-2, β-defensins and sIgA, and regulate the intestinal immunological barrier function by stimulating the production of cytokines and the functional development of immune cells.					
30553308	1	55	theme	microbiota	266:275	arg1	composition					277:287	microbiota composition	266:287	microbiota composition	266:287	The present study investigated the effects of a homogeneous Dendrobium huoshanense polysaccharide (GXG) on mucosal barrier function and microbiota composition in different intestinal regions of mice.					
31531180	2	0	theme	gel-permeation	468:481	arg1	chromatography					483:496	high-performance gel-permeation chromatography	451:496	high-performance gel-permeation chromatography (HPGPC)	451:504	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	0	theme	gel-permeation	468:481	arg1	HPGPC					499:503	HPGPC	499:503	HPGPC	499:503	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	5	1	theme	obvious	928:934	arg1	capacity					936:943	obvious capacity	928:943	obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical	928:1012	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	2	2	theme	scanning	362:369	arg1	SEM					392:394	SEM	392:394	SEM	392:394	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	2	theme	scanning	362:369	arg1	microscope					380:389	scanning electron microscope	362:389	scanning electron microscope (SEM)	362:395	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	5	3	theme	superoxide	995:1004	arg1	radical					1006:1012	superoxide radical	995:1012	superoxide radical	995:1012	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	0	4	theme	Mist	87:90	arg1	Tea					92:94	Yingshan Cloud Mist Tea	72:94	Yingshan Cloud Mist Tea	72:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	0	5	from	Composition	9:19	arg1	Tea					92:94	Yingshan Cloud Mist Tea	72:94	Yingshan Cloud Mist Tea	72:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	6	theme	antioxidant	164:174	arg1	activities					176:185	antioxidant activities	164:185	antioxidant activities	164:185	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	3	7	theme	antioxidant	598:608	arg1	activities					610:619	the antioxidant activities	594:619	the antioxidant activities in chickens	594:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	4	8	theme	molar	795:799	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	3	9	theme	GTPS	569:572	arg1	activities					610:619	the antioxidant activities	594:619	the antioxidant activities in chickens	594:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	9	theme	GTPS	569:572	arg1	activities					529:538	the antioxidant activities	513:538	the antioxidant activities in vitro of GTPS	513:555	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	9	theme	GTPS	569:572	arg1	effects					558:564	effects	558:564	effects of GTPS on body weight	558:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	10	from	activities	529:538	arg1	chickens					624:631	chickens	624:631	chickens	624:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	10	from	activities	529:538	arg1	weight					582:587	body weight	577:587	body weight	577:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	4	11	theme	 26.1 	816:821	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	6	12	theme	SOD	1147:1149	arg1	level					1163:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	1	13	from	composition	148:158	arg1	Tea					231:233	Yingshan Cloud Mist Tea	211:233	Yingshan Cloud Mist Tea	211:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	3	14	from	activities	610:619	arg1	chickens					624:631	chickens	624:631	chickens	624:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	14	from	activities	610:619	arg1	weight					582:587	body weight	577:587	body weight	577:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	2	15	theme	high-performance	451:466	arg1	chromatography					483:496	high-performance gel-permeation chromatography	451:496	high-performance gel-permeation chromatography (HPGPC)	451:504	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	15	theme	high-performance	451:466	arg1	HPGPC					499:503	HPGPC	499:503	HPGPC	499:503	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	4	16	theme	 30.7 	835:840	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	0	17	from	Activities	37:46	arg1	Tea					92:94	Yingshan Cloud Mist Tea	72:94	Yingshan Cloud Mist Tea	72:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	2	18	theme	infrared	331:338	arg1	spectroscopy					348:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	1	19	theme	polysaccharides	190:204	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	19	theme	polysaccharides	190:204	arg1	activities					176:185	antioxidant activities	164:185	antioxidant activities	164:185	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	0	20	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	5	21	theme	hydroxyl	973:980	arg1	radical					982:988	hydroxyl radical	973:988	hydroxyl radical	973:988	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	6	22	from	content	1188:1194	arg1	chickens					1206:1213	chickens	1206:1213	chickens	1206:1213	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	2	23	theme	Fourier-transform	313:329	arg1	spectroscopy					348:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	0	24	theme	Antioxidant	25:35	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	5	25	theme	radical	982:988	arg1	capacity					936:943	obvious capacity	928:943	obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical	928:1012	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	6	26	theme	T-AOC	1140:1144	arg1	level					1163:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	6	27	theme	GSH-Px	1156:1161	arg1	level					1163:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	the T-AOC, SOD, and GSH-Px level	1136:1167	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	3	28	from	effects	558:564	arg1	chickens					624:631	chickens	624:631	chickens	624:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	28	from	effects	558:564	arg1	weight					582:587	body weight	577:587	body weight	577:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	1	29	theme	Yingshan	211:218	arg1	Tea					231:233	Yingshan Cloud Mist Tea	211:233	Yingshan Cloud Mist Tea	211:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	3	30	theme	GTPS	552:555	arg1	activities					610:619	the antioxidant activities	594:619	the antioxidant activities in chickens	594:631	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	30	theme	GTPS	552:555	arg1	activities					529:538	the antioxidant activities	513:538	the antioxidant activities in vitro of GTPS	513:555	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	30	theme	GTPS	552:555	arg1	effects					558:564	effects	558:564	effects of GTPS on body weight	558:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	7	31	theme	feed	1327:1330	arg1	industry					1332:1339	feed industry	1327:1339	feed industry	1327:1339	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	7	32	from	application	1312:1322	arg1	industry					1332:1339	feed industry	1327:1339	feed industry	1327:1339	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	0	33	from	Tea	92:94	arg1	Polysaccharides					51:65	Polysaccharides	51:65	Polysaccharides from Yingshan Cloud Mist Tea	51:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	0	33	from	Tea	92:94	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	0	33	from	Tea	92:94	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	34	dep	composition	148:158	arg1	the					135:137	the	135:137	the	135:137	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	35	theme	Cloud	220:224	arg1	Tea					231:233	Yingshan Cloud Mist Tea	211:233	Yingshan Cloud Mist Tea	211:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	36	theme	Mist	226:229	arg1	Tea					231:233	Yingshan Cloud Mist Tea	211:233	Yingshan Cloud Mist Tea	211:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	4	37	theme	 1.9 	823:827	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	4	38	theme	 3.0 	829:833	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	5	39	theme	radical	1006:1012	arg1	capacity					936:943	obvious capacity	928:943	obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical	928:1012	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	2	40	theme	gas	423:425	arg1	chromatograph					427:439	gas chromatograph	423:439	gas chromatograph (GC)	423:444	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	40	theme	gas	423:425	arg1	GC					442:443	GC	442:443	GC	442:443	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	7	41	contain	had	1294:1296	arg1	kind					1259:1262	a kind	1257:1262	a kind	1257:1262	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	7	41	contain	had	1294:1296	arg1	GTPS					1243:1246	GTPS	1243:1246	GTPS	1243:1246	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	7	41	contain	had	1294:1296	arg2	application					1312:1322	the potential application	1298:1322	the potential application in feed industry	1298:1339	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	5	42	theme	scavenging	948:957	arg1	radical					964:970	scavenging DPPH radical	948:970	scavenging DPPH radical	948:970	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	6	43	theme	chickens	1117:1124	arg1	weight					1107:1112	the body weight	1098:1112	the body weight of chickens	1098:1124	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	4	44	theme	11.4 	810:814	arg1	ratio					801:805	a molar ratio	793:805	a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8	793:846	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	1	45	from	Tea	231:233	arg1	polysaccharides					190:204	polysaccharides	190:204	polysaccharides from Yingshan Cloud Mist Tea	190:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	45	from	Tea	231:233	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	1	45	from	Tea	231:233	arg1	activities					176:185	antioxidant activities	164:185	antioxidant activities	164:185	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	7	46	theme	potential	1302:1310	arg1	application					1312:1322	the potential application	1298:1322	the potential application in feed industry	1298:1339	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	0	47	theme	Polysaccharides	51:65	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	0	47	theme	Polysaccharides	51:65	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	5	48	theme	radical	964:970	arg1	capacity					936:943	obvious capacity	928:943	obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical	928:1012	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	7	49	theme	natural	1267:1273	arg1	antioxidant					1275:1285	natural antioxidant	1267:1285	natural antioxidant	1267:1285	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	7	50	theme	antioxidant	1275:1285	arg1	kind					1259:1262	a kind	1257:1262	a kind	1257:1262	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	7	50	theme	antioxidant	1275:1285	arg1	GTPS					1243:1246	GTPS	1243:1246	GTPS	1243:1246	The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.					
31531180	6	51	theme	MDA	1199:1201	arg1	content					1188:1194	the content	1184:1194	the content of MDA in chickens	1184:1213	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	2	52	theme	polysaccharides	274:288	arg1	composition					249:259	The chemical composition	236:259	The chemical composition of green tea polysaccharides (GTPS)	236:295	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	53	theme	chemical	240:247	arg1	composition					249:259	The chemical composition	236:259	The chemical composition of green tea polysaccharides (GTPS)	236:295	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	54	theme	FT-IR	341:345	arg1	spectroscopy					348:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	Fourier-transform infrared (FT-IR) spectroscopy	313:359	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	6	55	theme	body	1102:1105	arg1	weight					1107:1112	the body weight	1098:1112	the body weight of chickens	1098:1124	Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens.					
31531180	2	56	theme	tea	270:272	arg1	GTPS					291:294	GTPS	291:294	GTPS	291:294	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	56	theme	tea	270:272	arg1	polysaccharides					274:288	green tea polysaccharides	264:288	green tea polysaccharides (GTPS)	264:295	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	5	57	theme	ferric-reducing	1031:1045	arg1	power					1047:1051	the ferric-reducing power	1027:1051	the ferric-reducing power	1027:1051	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
31531180	0	58	theme	Cloud	81:85	arg1	Tea					92:94	Yingshan Cloud Mist Tea	72:94	Yingshan Cloud Mist Tea	72:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	4	59	theme	molecular	864:872	arg1	weight					874:879	the average molecular weight	852:879	the average molecular weight	852:879	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	4	59	theme	molecular	864:872	arg1	104 Da					892:897	9.69 × 104 Da	885:897	9.69 × 104 Da	885:897	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	2	60	theme	green	264:268	arg1	tea					270:272	green tea	264:272	green tea polysaccharides (GTPS)	264:295	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	1	61	theme	chemical	139:146	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	4	62	theme	×	890:890	arg1	weight					874:879	the average molecular weight	852:879	the average molecular weight	852:879	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	4	62	theme	×	890:890	arg1	104 Da					892:897	9.69 × 104 Da	885:897	9.69 × 104 Da	885:897	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	0	63	theme	Yingshan	72:79	arg1	Tea					92:94	Yingshan Cloud Mist Tea	72:94	Yingshan Cloud Mist Tea	72:94	Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea.					
31531180	3	64	theme	antioxidant	517:527	arg1	activities					529:538	the antioxidant activities	513:538	the antioxidant activities in vitro of GTPS	513:555	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	3	65	theme	body	577:580	arg1	weight					582:587	body weight	577:587	body weight	577:587	Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied.					
31531180	1	66	from	activities	176:185	arg1	Tea					231:233	Yingshan Cloud Mist Tea	211:233	Yingshan Cloud Mist Tea	211:233	The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea.					
31531180	2	67	theme	electron	371:378	arg1	SEM					392:394	SEM	392:394	SEM	392:394	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	2	67	theme	electron	371:378	arg1	microscope					380:389	scanning electron microscope	362:389	scanning electron microscope (SEM)	362:395	The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC).					
31531180	4	68	theme	average	856:862	arg1	weight					874:879	the average molecular weight	852:879	the average molecular weight	852:879	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	4	68	theme	average	856:862	arg1	104 Da					892:897	9.69 × 104 Da	885:897	9.69 × 104 Da	885:897	The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da.					
31531180	5	69	theme	DPPH	959:962	arg1	radical					964:970	scavenging DPPH radical	948:970	scavenging DPPH radical	948:970	Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro.					
29954171	2	0	theme	filament	591:598	arg1	states					600:605	both the wet gel filament and the dry filament states	553:605	states	600:605	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	3	1	theme	charged	689:695	arg1	gels					713:716	charged polyelectrolyte gels	689:716	charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions	689:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	6	2	theme	tensile	1491:1497	arg1	properties					1499:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	2	theme	tensile	1491:1497	arg1	dependent					1523:1531	dependent	1523:1531	dependent	1523:1531	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	5	3	theme	dyads	1236:1240	arg1	ratio					1212:1216	the compositional ratio	1194:1216	the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration	1194:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	5	4	theme	elastic	1401:1407	arg1	materials					1415:1423	elastic solid materials	1401:1423	elastic solid materials	1401:1423	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	5	theme	classical	731:739	arg1	elasticity					748:757	the classical rubber elasticity	727:757	the classical rubber elasticity	727:757	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	6	6	theme	stress	1590:1595	arg1	ratio					1581:1585	the ratio	1577:1585	the ratio of stress to alginate concentration	1577:1621	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	6	theme	stress	1590:1595	arg1	conditions					1552:1561	the preparation conditions	1536:1561	the preparation conditions	1536:1561	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	6	theme	stress	1590:1595	arg1	nature					1631:1636	the nature	1627:1636	the nature of the ions present during filament drawing	1627:1680	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	5	7	theme	compositional	1198:1210	arg1	ratio					1212:1216	the compositional ratio	1194:1216	the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration	1194:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	8	theme	dilated	762:768	arg1	networks					778:785	dilated polymer networks	762:785	dilated polymer networks	762:785	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	5	9	from	ratio	1212:1216	arg1	fraction					1258:1265	the alginate fraction	1245:1265	the alginate fraction multiplied by the alginate concentration	1245:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	1	10	theme	uronic	205:210	arg1	compositions					217:228	uronic acid compositions	205:228	uronic acid compositions	205:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	7	11	theme	PolyAl	1687:1692	arg1	solution					1694:1701	The PolyAl solution	1683:1701	The PolyAl solution	1683:1701	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	2	12	theme	filament	570:577	arg1	states					600:605	both the wet gel filament and the dry filament states	553:605	states	600:605	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	13	theme	charged	1103:1109	arg1	gels					1111:1114	charged gels	1103:1114	charged gels	1103:1114	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	2	14	theme	wet	562:564	arg1	filament					570:577	wet gel filament	562:577	wet gel filament	562:577	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	15	from	dyads	1236:1240	arg1	fraction					1258:1265	the alginate fraction	1245:1265	the alginate fraction multiplied by the alginate concentration	1245:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	16	theme	wet	612:614	arg1	model					620:624	The wet gel model	608:624	The wet gel model	608:624	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	4	17	theme	equilibrium	919:929	arg1	pressure					940:947	equilibrium swelling pressure	919:947	equilibrium swelling pressure	919:947	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	3	18	theme	networks	778:785	arg1	elasticity					748:757	the classical rubber elasticity	727:757	the classical rubber elasticity	727:757	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	4	19	theme	pressure	940:947	arg1	theory					909:914	The theory	905:914	The theory of equilibrium swelling pressure	905:947	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	5	20	from	fraction	1258:1265	arg1	ratio					1212:1216	the compositional ratio	1194:1216	the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration	1194:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	1	21	theme	fractions	142:150	arg1	series					123:128	A series	121:128	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions	121:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	4	22	theme	alginate	1003:1010	arg1	gels					1012:1015	the alginate gels	999:1015	the alginate gels	999:1015	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	5	23	theme	gel	1148:1150	arg1	filaments					1152:1160	the gel filaments	1144:1160	the gel filaments prepared from CaCl2	1144:1180	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	6	24	theme	alginate	1600:1607	arg1	concentration					1609:1621	alginate concentration	1600:1621	alginate concentration	1600:1621	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	1	25	theme	significant	157:167	arg1	differences					169:179	significant differences	157:179	significant differences in molecular weight and uronic acid compositions	157:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	5	26	theme	alginate	1285:1292	arg1	concentration					1294:1306	the alginate concentration	1281:1306	the alginate concentration	1281:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	27	theme	extensions	848:857	arg1	contributions					812:824	the contributions	808:824	the contributions of non-Gaussian chain extensions	808:857	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	3	27	theme	extensions	848:857	arg1	effect					867:872	the effect	863:872	the effect of electrostatic interactions	863:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	0	28	theme	Theoretical	85:95	arg1	Approach					111:118	A Theoretical and Practical Approach	83:118	A Theoretical and Practical Approach	83:118	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	1	29	from	differences	169:179	arg1	compositions					217:228	uronic acid compositions	205:228	uronic acid compositions	205:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	1	29	from	differences	169:179	arg1	weight					194:199	molecular weight	184:199	molecular weight	184:199	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	3	30	theme	interactions	891:902	arg1	contributions					812:824	the contributions	808:824	the contributions of non-Gaussian chain extensions	808:857	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	3	30	theme	interactions	891:902	arg1	effect					867:872	the effect	863:872	the effect of electrostatic interactions	863:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	4	31	theme	observed	977:984	arg1	shrinkage					986:994	the observed shrinkage	973:994	the observed shrinkage of the alginate gels	973:1015	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	3	32	theme	non-Gaussian	829:840	arg1	extensions					848:857	non-Gaussian chain extensions	829:857	non-Gaussian chain extensions	829:857	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	0	33	theme	Practical	101:109	arg1	Approach					111:118	A Theoretical and Practical Approach	83:118	A Theoretical and Practical Approach	83:118	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	1	34	theme	polyaluminum	378:389	arg1	solutions					411:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions	356:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying	356:450	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	1	35	with	fractions	142:150	arg1	differences					169:179	significant differences	157:179	significant differences in molecular weight and uronic acid compositions	157:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	6	36	theme	filament	1665:1672	arg1	drawing					1674:1680	filament drawing	1665:1680	filament drawing	1665:1680	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	0	37	from	Alginate	45:52	arg1	Filaments					17:25	High-Performance Filaments	0:25	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking	0:80	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	0	38	theme	High-Performance	0:15	arg1	Filaments					17:25	High-Performance Filaments	0:25	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking	0:80	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	1	39	theme	strong	299:304	arg1	filaments					327:335	thin and strong cross-linked polymer filaments	290:335	thin and strong cross-linked polymer filaments	290:335	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	6	40	theme	present	1650:1656	arg1	ions					1645:1648	the ions	1641:1648	the ions present during filament drawing	1641:1680	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	5	41	theme	wet	1339:1341	arg1	filaments					1347:1355	wet gel filaments	1339:1355	wet gel filaments prepared by AlCl3	1339:1373	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	1	42	theme	polymer	319:325	arg1	filaments					327:335	thin and strong cross-linked polymer filaments	290:335	thin and strong cross-linked polymer filaments	290:335	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	2	43	theme	uronic	499:504	arg1	composition					511:521	the alginate uronic acid composition	486:521	the alginate uronic acid composition	486:521	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	44	theme	filaments	1347:1355	arg1	behavior					1327:1334	the tensile behavior	1315:1334	the tensile behavior of wet gel filaments prepared by AlCl3	1315:1373	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	7	45	theme	elastic	1889:1895	arg1	moduli					1897:1902	elastic moduli	1889:1902	elastic moduli	1889:1902	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	4	46	theme	polyvalent	1042:1051	arg1	solution					1053:1060	a polyvalent solution	1040:1060	a polyvalent solution	1040:1060	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	0	47	theme	Polyvalent	57:66	arg1	Cross-Linking					68:80	Polyvalent Cross-Linking	57:80	Polyvalent Cross-Linking	57:80	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	7	48	dep	able	1826:1829	arg1	having					1882:1887	having	1882:1887	having elastic moduli as high as 28 GPa	1882:1920	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	7	48	dep	able	1826:1829	arg1	withstand					1834:1842	withstand	1834:1842	to withstand stresses of approximately 500 MPa	1831:1876	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	5	49	theme	alginate	1249:1256	arg1	fraction					1258:1265	the alginate fraction	1245:1265	the alginate fraction multiplied by the alginate concentration	1245:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	50	theme	unidirectional	660:673	arg1	elongation					675:684	the unidirectional elongation	656:684	the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions	656:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	1	51	link	cross-linked	306:317	arg1	filaments					327:335	thin and strong cross-linked polymer filaments	290:335	thin and strong cross-linked polymer filaments	290:335	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	5	52	theme	acid	1231:1234	arg1	dyads					1236:1240	guluronic acid dyads	1221:1240	guluronic acid dyads in the alginate fraction multiplied by the alginate concentration	1221:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	5	53	theme	filaments	1152:1160	arg1	performance					1129:1139	the tensile performance	1117:1139	the tensile performance of the gel filaments prepared from CaCl2	1117:1180	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	5	54	with	Congruent	1063:1071	arg1	model					1094:1098	the theoretical model	1078:1098	the theoretical model of charged gels	1078:1114	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	55	theme	polyelectrolyte	697:711	arg1	gels					713:716	charged polyelectrolyte gels	689:716	charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions	689:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	6	56	theme	filament	1482:1489	arg1	properties					1499:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	56	theme	filament	1482:1489	arg1	dependent					1523:1531	dependent	1523:1531	dependent	1523:1531	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	1	57	theme	thin	290:293	arg1	filaments					327:335	thin and strong cross-linked polymer filaments	290:335	thin and strong cross-linked polymer filaments	290:335	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	3	58	theme	rubber	741:746	arg1	elasticity					748:757	the classical rubber elasticity	727:757	the classical rubber elasticity	727:757	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	1	59	theme	molecular	184:192	arg1	weight					194:199	molecular weight	184:199	molecular weight	184:199	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	7	60	theme	stiff	1810:1814	arg1	materials					1816:1824	highly stiff materials	1803:1824	highly stiff materials	1803:1824	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	4	61	theme	gels	1012:1015	arg1	shrinkage					986:994	the observed shrinkage	973:994	the observed shrinkage of the alginate gels	973:1015	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	3	62	theme	polymer	770:776	arg1	networks					778:785	dilated polymer networks	762:785	dilated polymer networks	762:785	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	5	63	theme	alginate	1450:1457	arg1	concentration					1459:1471	the alginate concentration	1446:1471	the alginate concentration	1446:1471	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	1	64	theme	acid	212:215	arg1	compositions					217:228	uronic acid compositions	205:228	uronic acid compositions	205:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	2	65	theme	gel	566:568	arg1	filament					570:577	wet gel filament	562:577	wet gel filament	562:577	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	66	theme	gels	1111:1114	arg1	model					1094:1098	the theoretical model	1078:1098	the theoretical model of charged gels	1078:1114	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	6	67	theme	preparation	1540:1550	arg1	ratio					1581:1585	the ratio	1577:1585	the ratio of stress to alginate concentration	1577:1621	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	67	theme	preparation	1540:1550	arg1	conditions					1552:1561	the preparation conditions	1536:1561	the preparation conditions	1536:1561	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	67	theme	preparation	1540:1550	arg1	nature					1631:1636	the nature	1627:1636	the nature of the ions present during filament drawing	1627:1680	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	2	68	theme	dry	587:589	arg1	filament					591:598	the dry filament	583:598	the dry filament	583:598	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	2	69	from	performance	538:548	arg1	states					600:605	both the wet gel filament and the dry filament states	553:605	states	600:605	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	70	theme	theoretical	1082:1092	arg1	model					1094:1098	the theoretical model	1078:1098	the theoretical model of charged gels	1078:1114	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	1	71	theme	alginate	133:140	arg1	fractions					142:150	alginate fractions	133:150	alginate fractions with significant differences in molecular weight and uronic acid compositions	133:228	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	3	72	theme	electrostatic	877:889	arg1	interactions					891:902	electrostatic interactions	877:902	electrostatic interactions	877:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	4	73	theme	swelling	931:938	arg1	pressure					940:947	equilibrium swelling pressure	919:947	equilibrium swelling pressure	919:947	The theory of equilibrium swelling pressure was applied to describe the observed shrinkage of the alginate gels following immersion in a polyvalent solution.					
29954171	5	74	theme	tensile	1319:1325	arg1	behavior					1327:1334	the tensile behavior	1315:1334	the tensile behavior of wet gel filaments prepared by AlCl3	1315:1373	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	75	theme	gel	616:618	arg1	model					620:624	The wet gel model	608:624	The wet gel model	608:624	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	7	76	theme	resulting	1772:1780	arg1	filaments					1782:1790	the resulting filaments	1768:1790	the resulting filaments	1768:1790	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	1	77	theme	aluminum	365:372	arg1	solutions					411:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions	356:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying	356:450	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	7	78	theme	strong	1749:1754	arg1	extent					1756:1761	a strong extent	1747:1761	a strong extent	1747:1761	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	5	79	theme	tensile	1121:1127	arg1	performance					1129:1139	the tensile performance	1117:1139	the tensile performance of the gel filaments prepared from CaCl2	1117:1180	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	2	80	theme	acid	506:509	arg1	composition					511:521	the alginate uronic acid composition	486:521	the alginate uronic acid composition	486:521	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	1	81	theme	polyvalent	400:409	arg1	solutions					411:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions	356:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying	356:450	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	3	82	theme	chain	842:846	arg1	extensions					848:857	non-Gaussian chain extensions	829:857	non-Gaussian chain extensions	829:857	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	0	83	dep	Approach	111:118	arg1	Filaments					17:25	High-Performance Filaments	0:25	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking	0:80	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	2	84	theme	tensile	530:536	arg1	performance					538:548	the tensile performance	526:548	the tensile performance in both the wet gel filament and the dry filament states	526:605	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	6	85	theme	dry	1478:1480	arg1	properties					1499:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties	1474:1508	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	85	theme	dry	1478:1480	arg1	dependent					1523:1531	dependent	1523:1531	dependent	1523:1531	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	7	86	theme	alginate	1735:1742	arg1	shrinkage					1722:1730	shrinkage	1722:1730	shrinkage of alginate to a strong extent	1722:1761	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	0	87	theme	Fractionated	32:43	arg1	Alginate					45:52	Fractionated Alginate	32:52	Fractionated Alginate by Polyvalent Cross-Linking	32:80	High-Performance Filaments from Fractionated Alginate by Polyvalent Cross-Linking: A Theoretical and Practical Approach.					
29954171	1	88	theme	cross-linked	306:317	arg1	filaments					327:335	thin and strong cross-linked polymer filaments	290:335	thin and strong cross-linked polymer filaments	290:335	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	6	89	theme	ions	1645:1648	arg1	ratio					1581:1585	the ratio	1577:1585	the ratio of stress to alginate concentration	1577:1621	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	89	theme	ions	1645:1648	arg1	conditions					1552:1561	the preparation conditions	1536:1561	the preparation conditions	1536:1561	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	6	89	theme	ions	1645:1648	arg1	nature					1631:1636	the nature	1627:1636	the nature of the ions present during filament drawing	1627:1680	The dry filament tensile properties were greatly dependent on the preparation conditions, particularly the ratio of stress to alginate concentration and the nature of the ions present during filament drawing.					
29954171	5	90	theme	gel	1343:1345	arg1	filaments					1347:1355	wet gel filaments	1339:1355	wet gel filaments prepared by AlCl3	1339:1373	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	5	91	theme	guluronic	1221:1229	arg1	dyads					1236:1240	guluronic acid dyads	1221:1240	guluronic acid dyads in the alginate fraction multiplied by the alginate concentration	1221:1306	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	2	92	theme	alginate	490:497	arg1	composition					511:521	the alginate uronic acid composition	486:521	the alginate uronic acid composition	486:521	Models were elaborated to relate the alginate uronic acid composition to the tensile performance in both the wet gel filament and the dry filament states.					
29954171	5	93	theme	solid	1409:1413	arg1	materials					1415:1423	elastic solid materials	1401:1423	elastic solid materials	1401:1423	Congruent with the theoretical model of charged gels, the tensile performance of the gel filaments prepared from CaCl2 depended on the compositional ratio of guluronic acid dyads in the alginate fraction multiplied by the alginate concentration, while the tensile behavior of wet gel filaments prepared by AlCl3 instead resembled that of elastic solid materials and depended only on the alginate concentration.					
29954171	3	94	theme	elongation	675:684	arg1	theory					646:651	the theory	642:651	the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions	642:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29954171	1	95	theme	calcium	356:362	arg1	solutions					411:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions	356:419	calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying	356:450	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	1	96	theme	consecutive	247:257	arg1	fractionation					259:271	consecutive fractionation	247:271	consecutive fractionation	247:271	A series of alginate fractions with significant differences in molecular weight and uronic acid compositions were produced by consecutive fractionation and converted to thin and strong cross-linked polymer filaments via extrusion into calcium, aluminum, or polyaluminum (PolyAl) polyvalent solutions followed by drawing and drying.					
29954171	7	97	theme	MPa	1874:1876	arg1	stresses					1844:1851	stresses	1844:1851	stresses of approximately 500 MPa	1844:1876	The PolyAl solution effectively caused shrinkage of alginate to a strong extent, and the resulting filaments behaved as highly stiff materials able to withstand stresses of approximately 500 MPa and having elastic moduli as high as 28 GPa.					
29954171	3	98	theme	gels	713:716	arg1	elongation					675:684	the unidirectional elongation	656:684	the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions	656:902	The wet gel model was compared to the theory of the unidirectional elongation of charged polyelectrolyte gels based on the classical rubber elasticity of dilated polymer networks, extended to include the contributions of non-Gaussian chain extensions and the effect of electrostatic interactions.					
29492483	8	0	theme	straightforward	1656:1670	arg1	route					1672:1676	a straightforward route	1654:1676	a straightforward route for the preparation of such compounds	1654:1714	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	3	1	theme	polyprenol-pyrophosphate	568:591	arg1	substrates					618:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates	568:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate	568:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	0	2	theme	biosynthetic	111:122	arg1	enzymes					124:130	mycobacterial galactan biosynthetic enzymes	88:130	mycobacterial galactan biosynthetic enzymes	88:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	6	3	dep	GlfT2	1067:1071	arg1	3					1074:1074	3	1074:1074	3	1074:1074	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	6	3	dep	GlfT2	1067:1071	arg1	4					1080:1080	4	1080:1080	4	1080:1080	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	6	4	theme	alkyl	1145:1149	arg1	substrates					1161:1170	the corresponding alkyl glycoside substrates	1127:1170	the corresponding alkyl glycoside substrates reported previously	1127:1190	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	2	5	theme	galactan	389:396	arg1	domain					398:403	its core a galactan domain	378:403	its core a galactan domain composed of d-galactofuranose residues	378:442	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	8	6	from	importance	1546:1555	arg1	recognition					1587:1597	recognition	1587:1597	recognition of acceptor substrates by both enzymes	1587:1636	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	3	7	theme	Mycobacterial	445:457	arg1	biosynthesis					468:479	Mycobacterial galactan biosynthesis	445:479	Mycobacterial galactan biosynthesis	445:479	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	0	8	link	linked	35:40	arg1	oligosaccharides					42:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	8	link	linked	35:40	arg1	substrates					73:82	efficient substrates	63:82	efficient substrates for mycobacterial galactan biosynthetic enzymes	63:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	3	9	theme	donor	662:666	arg1	UDP-galactofuranose					635:653	UDP-galactofuranose	635:653	UDP-galactofuranose	635:653	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	3	9	theme	donor	662:666	arg1	substrate					668:676	the donor substrate	658:676	the donor substrate	658:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	8	10	theme	compounds	1706:1714	arg1	preparation					1686:1696	the preparation	1682:1696	the preparation of such compounds	1682:1714	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	7	11	theme	respective	1290:1299	arg1	enzyme					1309:1314	the respective cognate enzyme	1286:1314	the respective cognate enzyme	1286:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	7	12	theme	23	1278:1279	arg1	reaction					1253:1260	the reaction	1249:1260	the reaction of 3, 4, 22 and 23 with the respective cognate enzyme	1249:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	6	13	theme	corresponding	1131:1143	arg1	substrates					1161:1170	the corresponding alkyl glycoside substrates	1127:1170	the corresponding alkyl glycoside substrates reported previously	1127:1190	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	9	14	theme	close	1851:1855	arg1	analogs					1868:1874	close structural analogs	1851:1874	close structural analogs	1851:1874	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
29492483	7	15	theme	cognate	1301:1307	arg1	enzyme					1309:1314	the respective cognate enzyme	1286:1314	the respective cognate enzyme	1286:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	8	16	theme	motif	1578:1582	arg1	importance					1546:1555	the importance	1542:1555	the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes	1542:1636	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	7	17	theme	products	1228:1235	arg1	analysis					1212:1219	Mass spectrometric analysis	1193:1219	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme	1193:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	7	17	theme	products	1228:1235	arg1	UDP-galactofuranose					1320:1338	UDP-galactofuranose	1320:1338	UDP-galactofuranose	1320:1338	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	5	18	theme	oligosaccharide	887:901	arg1	coupling					955:962	coupling	955:962	coupling to a polyprenol phosphate	955:988	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	5	18	theme	oligosaccharide	887:901	arg1	synthesis					871:879	chemical synthesis	862:879	chemical synthesis of an oligosaccharide	862:901	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	5	18	theme	oligosaccharide	887:901	arg1	phosphorylation					915:929	subsequent phosphorylation	904:929	subsequent phosphorylation at the reducing end	904:949	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	2	19	theme	wall	314:317	arg1	component					292:300	The largest structural component	269:300	The largest structural component of the cell wall	269:317	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	2	19	theme	wall	314:317	arg1	complex					362:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	9	20	theme	structural	1857:1866	arg1	analogs					1868:1874	close structural analogs	1851:1874	close structural analogs	1851:1874	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
29492483	1	21	theme	complex	214:220	arg1	wall					227:230	a complex cell wall	212:230	a complex cell wall that is critical for their survival	212:266	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	1	21	theme	complex	214:220	arg1	critical					240:247	critical	240:247	critical	240:247	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	7	22	theme	Mass	1193:1196	arg1	analysis					1212:1219	Mass spectrometric analysis	1193:1219	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme	1193:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	5	23	theme	chemical	862:869	arg1	synthesis					871:879	chemical synthesis	862:879	chemical synthesis of an oligosaccharide	862:901	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	2	24	theme	cell	309:312	arg1	wall					314:317	the cell wall	305:317	the cell wall	305:317	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	1	25	theme	cell	222:225	arg1	wall					227:230	a complex cell wall	212:230	a complex cell wall that is critical for their survival	212:266	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	1	25	theme	cell	222:225	arg1	critical					240:247	critical	240:247	critical	240:247	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	0	26	theme	polyprenol-pyrophosphate	10:33	arg1	oligosaccharides					42:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	26	theme	polyprenol-pyrophosphate	10:33	arg1	substrates					73:82	efficient substrates	63:82	efficient substrates for mycobacterial galactan biosynthetic enzymes	63:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	27	theme	Synthetic	0:8	arg1	oligosaccharides					42:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	27	theme	Synthetic	0:8	arg1	substrates					73:82	efficient substrates	63:82	efficient substrates for mycobacterial galactan biosynthetic enzymes	63:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	8	28	theme	substrates	1611:1620	arg1	recognition					1587:1597	recognition	1587:1597	recognition of acceptor substrates by both enzymes	1587:1636	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	8	29	theme	such	1701:1704	arg1	compounds					1706:1714	such compounds	1701:1714	such compounds	1701:1714	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	2	30	theme	d-galactofuranose	417:433	arg1	residues					435:442	d-galactofuranose residues	417:442	d-galactofuranose residues	417:442	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	5	31	theme	subsequent	904:913	arg1	phosphorylation					915:929	subsequent phosphorylation	904:929	subsequent phosphorylation at the reducing end	904:949	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	8	32	theme	acceptor	1602:1609	arg1	substrates					1611:1620	acceptor substrates	1602:1620	acceptor substrates	1602:1620	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	7	33	theme	galactofuranose	1438:1452	arg1	residues					1454:1461	the first two galactofuranose residues	1424:1461	the first two galactofuranose residues	1424:1461	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	7	34	theme	first	1428:1432	arg1	residues					1454:1461	the first two galactofuranose residues	1424:1461	the first two galactofuranose residues	1424:1461	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	7	35	theme	additional	1348:1357	arg1	evidence					1359:1366	additional evidence	1348:1366	additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2	1348:1506	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	0	36	theme	linked	35:40	arg1	oligosaccharides					42:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	36	theme	linked	35:40	arg1	substrates					73:82	efficient substrates	63:82	efficient substrates for mycobacterial galactan biosynthetic enzymes	63:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	3	37	link	linked	593:598	arg1	substrates					618:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates	568:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate	568:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	2	38	theme	mycolyl-arabinogalactan-peptidoglycan	324:360	arg1	complex					362:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	2	38	theme	mycolyl-arabinogalactan-peptidoglycan	324:360	arg1	component					292:300	The largest structural component	269:300	The largest structural component of the cell wall	269:317	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	4	39	theme	GlfT1	726:730	arg1	substrates					751:760	GlfT1 and GlfT2 acceptor substrates	726:760	GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	726:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	0	40	theme	efficient	63:71	arg1	oligosaccharides					42:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides	0:57	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	0	40	theme	efficient	63:71	arg1	substrates					73:82	efficient substrates	63:82	efficient substrates for mycobacterial galactan biosynthetic enzymes	63:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	2	41	theme	core	382:385	arg1	domain					398:403	its core a galactan domain	378:403	its core a galactan domain composed of d-galactofuranose residues	378:442	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	5	42	theme	reducing	938:945	arg1	end					947:949	the reducing end	934:949	the reducing end	934:949	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	2	43	theme	largest	273:279	arg1	component					292:300	The largest structural component	269:300	The largest structural component of the cell wall	269:317	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	2	43	theme	largest	273:279	arg1	complex					362:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	4	44	theme	pyrophosphate	773:785	arg1	compounds					812:820	compounds 3, 4, 22 and 23	812:836	compounds 3, 4, 22 and 23	812:836	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	4	44	theme	pyrophosphate	773:785	arg1	moieties					802:809	pyrophosphate and polyprenol moieties	773:809	pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	773:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	4	45	theme	acceptor	742:749	arg1	substrates					751:760	GlfT1 and GlfT2 acceptor substrates	726:760	GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	726:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	7	46	theme	galactan	1376:1383	arg1	model					1398:1402	the galactan biosynthetic model	1372:1402	the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2	1372:1506	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	9	47	theme	natural	1883:1889	arg1	substrates					1891:1900	the natural substrates	1879:1900	the natural substrates	1879:1900	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
29492483	4	48	theme	GlfT2	736:740	arg1	substrates					751:760	GlfT1 and GlfT2 acceptor substrates	726:760	GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	726:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	3	49	theme	galactan	459:466	arg1	biosynthesis					468:479	Mycobacterial galactan biosynthesis	445:479	Mycobacterial galactan biosynthesis	445:479	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	5	50	theme	polyprenol	969:978	arg1	phosphate					980:988	a polyprenol phosphate	967:988	a polyprenol phosphate	967:988	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	6	51	theme	glycoside	1151:1159	arg1	substrates					1161:1170	the corresponding alkyl glycoside substrates	1127:1170	the corresponding alkyl glycoside substrates reported previously	1127:1190	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	7	52	theme	22	1271:1272	arg1	reaction					1253:1260	the reaction	1249:1260	the reaction of 3, 4, 22 and 23 with the respective cognate enzyme	1249:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	5	53	from	end	947:949	arg1	coupling					955:962	coupling	955:962	coupling to a polyprenol phosphate	955:988	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	5	53	from	end	947:949	arg1	synthesis					871:879	chemical synthesis	862:879	chemical synthesis of an oligosaccharide	862:901	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	5	53	from	end	947:949	arg1	phosphorylation					915:929	subsequent phosphorylation	904:929	subsequent phosphorylation at the reducing end	904:949	The approach involves chemical synthesis of an oligosaccharide, subsequent phosphorylation at the reducing end and coupling to a polyprenol phosphate.					
29492483	2	54	theme	a	387:387	arg1	domain					398:403	its core a galactan domain	378:403	its core a galactan domain composed of d-galactofuranose residues	378:442	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	7	55	theme	spectrometric	1198:1210	arg1	analysis					1212:1219	Mass spectrometric analysis	1193:1219	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme	1193:1314	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	9	56	theme	important	1789:1797	arg1	glycan					1799:1804	this important glycan	1784:1804	this important glycan	1784:1804	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
29492483	9	57	theme	additional	1740:1749	arg1	support					1751:1757	additional support	1740:1757	additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates	1740:1900	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
29492483	3	58	theme	linked	593:598	arg1	substrates					618:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates	568:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate	568:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	1	59	theme	human	161:165	arg1	tuberculosis					190:201	the human pathogen Mycobacterium tuberculosis	157:201	the human pathogen Mycobacterium tuberculosis	157:201	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	6	60	dep	GlfT1	1046:1050	arg1	23					1060:1061	23	1060:1061	23	1060:1061	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	6	60	dep	GlfT1	1046:1050	arg1	22					1053:1054	22	1053:1054	22	1053:1054	These compounds were shown to be substrates for either GlfT1 (22 and 23) or GlfT2 (3 and 4) and all were substantially more active than the corresponding alkyl glycoside substrates reported previously.					
29492483	4	61	theme	polyprenol	791:800	arg1	compounds					812:820	compounds 3, 4, 22 and 23	812:836	compounds 3, 4, 22 and 23	812:836	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	4	61	theme	polyprenol	791:800	arg1	moieties					802:809	pyrophosphate and polyprenol moieties	773:809	pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	773:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	1	62	theme	pathogen	167:174	arg1	tuberculosis					190:201	the human pathogen Mycobacterium tuberculosis	157:201	the human pathogen Mycobacterium tuberculosis	157:201	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	4	63	theme	chemical	704:711	arg1	synthesis					713:721	the first chemical synthesis	694:721	the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	694:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	1	64	theme	Mycobacterium	176:188	arg1	tuberculosis					190:201	the human pathogen Mycobacterium tuberculosis	157:201	the human pathogen Mycobacterium tuberculosis	157:201	Mycobacteria, including the human pathogen Mycobacterium tuberculosis, produce a complex cell wall that is critical for their survival.					
29492483	7	65	theme	biosynthetic	1385:1396	arg1	model					1398:1402	the galactan biosynthetic model	1372:1402	the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2	1372:1506	Mass spectrometric analysis of the products formed from the reaction of 3, 4, 22 and 23 with the respective cognate enzyme and UDP-galactofuranose provide additional evidence for the galactan biosynthetic model in which GlfT1 adds the first two galactofuranose residues with the remainder being installed via GlfT2.					
29492483	4	66	theme	first	698:702	arg1	synthesis					713:721	the first chemical synthesis	694:721	the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	694:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	0	67	theme	galactan	102:109	arg1	enzymes					124:130	mycobacterial galactan biosynthetic enzymes	88:130	mycobacterial galactan biosynthetic enzymes	88:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	4	68	contain	containing	762:771	arg2	compounds					812:820	compounds 3, 4, 22 and 23	812:836	compounds 3, 4, 22 and 23	812:836	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	4	68	contain	containing	762:771	arg2	moieties					802:809	pyrophosphate and polyprenol moieties	773:809	pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	773:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	4	68	contain	containing	762:771	arg1	substrates					751:760	GlfT1 and GlfT2 acceptor substrates	726:760	GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	726:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	3	69	theme	glycosyl	600:607	arg1	substrates					618:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates	568:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate	568:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	4	70	theme	substrates	751:760	arg1	synthesis					713:721	the first chemical synthesis	694:721	the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23)	694:837	We here report the first chemical synthesis of GlfT1 and GlfT2 acceptor substrates containing pyrophosphate and polyprenol moieties (compounds 3, 4, 22 and 23).					
29492483	0	71	theme	mycobacterial	88:100	arg1	enzymes					124:130	mycobacterial galactan biosynthetic enzymes	88:130	mycobacterial galactan biosynthetic enzymes	88:130	Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes.					
29492483	2	72	theme	structural	281:290	arg1	component					292:300	The largest structural component	269:300	The largest structural component of the cell wall	269:317	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	2	72	theme	structural	281:290	arg1	complex					362:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	the mycolyl-arabinogalactan-peptidoglycan complex	320:368	The largest structural component of the cell wall, the mycolyl-arabinogalactan-peptidoglycan complex, has at its core a galactan domain composed of d-galactofuranose residues.					
29492483	3	73	theme	acceptor	609:616	arg1	substrates					618:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates	568:627	polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate	568:676	Mycobacterial galactan biosynthesis has been proposed to involve two glycosyltransferases, GlfT1 and GlfT2, which elongate polyprenol-pyrophosphate linked glycosyl acceptor substrates using UDP-galactofuranose as the donor substrate.					
29492483	8	74	theme	pyrophosphate	1564:1576	arg1	motif					1578:1582	the pyrophosphate motif	1560:1582	the pyrophosphate motif	1560:1582	Overall, these results highlight the importance of the pyrophosphate motif in recognition of acceptor substrates by both enzymes and demonstrate a straightforward route for the preparation of such compounds.					
29492483	9	75	theme	first	1839:1843	arg1	time					1845:1848	the first time	1835:1848	the first time	1835:1848	The work also provides additional support for the process by which this important glycan is biosynthesized using, for the first time, close structural analogs to the natural substrates.					
31446105	9	0	theme	sucrose	1354:1360	arg1	pathway					1332:1338	the metabolic pathway	1318:1338	the metabolic pathway of starch and sucrose	1318:1360	Based on the metagenomic sequencing, it was found that FBTPS-3 significantly enriched the metabolic pathway of starch and sucrose.					
31446105	10	1	theme	physiological	1532:1544	arg1	functions					1556:1564	physiological metabolic functions	1532:1564	physiological metabolic functions	1532:1564	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	3	2	theme	gut	484:486	arg1	microbiota					488:497	gut microbiota	484:497	gut microbiota	484:497	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	0	3	from	fraction	9:16	arg1	tea					56:58	Fuzhuan brick tea	42:58	Fuzhuan brick tea	42:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	10	4	theme	functional	1431:1440	arg1	ingredients					1442:1452	functional ingredients	1431:1452	functional ingredients	1431:1452	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	10	4	theme	functional	1431:1440	arg1	FBTPS-3					1392:1398	FBTPS-3	1392:1398	FBTPS-3	1392:1398	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	8	5	theme	n-butyric	1215:1223	arg1	acids					1225:1229	acetic, propionic and n-butyric acids	1193:1229	acetic, propionic and n-butyric acids	1193:1229	FBTPS-3 also significantly promoted the production of acetic, propionic and n-butyric acids.					
31446105	1	6	theme	purified	159:166	arg1	fraction					168:175	One purified fraction	155:175	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS)	155:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	6	theme	purified	159:166	arg1	FBTPS-3					231:237	FBTPS-3	231:237	FBTPS-3	231:237	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	4	7	from	heteropolysaccharide	583:602	arg1	ratio					704:708	molar ratio	698:708	molar ratio of 8.7:15.5:42.2:19.7:13.9	698:735	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	6	8	theme	molecular	870:878	arg1	weight					880:885	the molecular weight	866:885	the molecular weight of FBTPS-3 and content of carbohydrates	866:925	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	7	9	theme	Prevotella	1087:1096	arg1	abundances					1044:1053	the relative abundances	1031:1053	the relative abundances of Bacteroides, Megasphaera and Prevotella	1031:1096	Furthermore, the relative abundances of Bacteroides, Megasphaera and Prevotella were significantly increased by FBTPS-3.					
31446105	2	10	dep	properties	325:334	arg1	The					312:314	The	312:314	The	312:314	The chemical properties and probiotic effects of FBTPS-3 were evaluated by fermentation in vitro.					
31446105	0	11	from	tea	56:58	arg1	polysaccharides					21:35	polysaccharides	21:35	polysaccharides from Fuzhuan brick tea	21:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	0	11	from	tea	56:58	arg1	fraction					9:16	Purified fraction	0:16	Purified fraction of polysaccharides from Fuzhuan brick tea	0:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	4	12	theme	8.7:15.5:42.2:19.7:13.9	713:735	arg1	ratio					704:708	molar ratio	698:708	molar ratio of 8.7:15.5:42.2:19.7:13.9	698:735	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	0	13	theme	microbiota	108:117	arg1	composition					74:84	composition	74:84	composition	74:84	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	0	13	theme	microbiota	108:117	arg1	metabolism					90:99	metabolism	90:99	metabolism	90:99	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	7	14	theme	Megasphaera	1071:1081	arg1	abundances					1044:1053	the relative abundances	1031:1053	the relative abundances of Bacteroides, Megasphaera and Prevotella	1031:1096	Furthermore, the relative abundances of Bacteroides, Megasphaera and Prevotella were significantly increased by FBTPS-3.					
31446105	4	15	theme	741 kDa	629:635	arg1	weight					619:624	molecular weight	609:624	molecular weight of 741 kDa, which was mainly composed of Man	609:669	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	0	16	theme	gut	104:106	arg1	microbiota					108:117	gut microbiota	104:117	gut microbiota	104:117	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	6	17	used	utilized	990:997	arg2	FBTPS-3					973:979	FBTPS-3	973:979	FBTPS-3	973:979	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	3	18	theme	metagenomic	520:530	arg1	sequencing					532:541	metagenomic sequencing	520:541	metagenomic sequencing	520:541	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	6	19	theme	content	902:908	arg1	weight					880:885	the molecular weight	866:885	the molecular weight of FBTPS-3 and content of carbohydrates	866:925	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	5	20	theme	acid	779:782	arg1	%					812:812	44.78 ± 2.85%	800:812	44.78 ± 2.85%	800:812	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	5	20	theme	acid	779:782	arg1	contents					742:749	The contents	738:749	The contents of carbohydrates and uronic acid in FBTPS-3	738:793	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	9	21	theme	metabolic	1322:1330	arg1	pathway					1332:1338	the metabolic pathway	1318:1338	the metabolic pathway of starch and sucrose	1318:1360	Based on the metagenomic sequencing, it was found that FBTPS-3 significantly enriched the metabolic pathway of starch and sucrose.					
31446105	7	22	theme	Bacteroides	1058:1068	arg1	abundances					1044:1053	the relative abundances	1031:1053	the relative abundances of Bacteroides, Megasphaera and Prevotella	1031:1096	Furthermore, the relative abundances of Bacteroides, Megasphaera and Prevotella were significantly increased by FBTPS-3.					
31446105	4	23	theme	molar	698:702	arg1	ratio					704:708	molar ratio	698:708	molar ratio of 8.7:15.5:42.2:19.7:13.9	698:735	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	1	24	theme	crude	182:186	arg1	FBTPS					223:227	FBTPS	223:227	FBTPS	223:227	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	24	theme	crude	182:186	arg1	polysaccharides					206:220	crude Fuzhuan brick tea polysaccharides	182:220	crude Fuzhuan brick tea polysaccharides (FBTPS)	182:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	0	25	theme	Purified	0:7	arg1	fraction					9:16	Purified fraction	0:16	Purified fraction of polysaccharides from Fuzhuan brick tea	0:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	1	26	theme	column	261:266	arg1	chromatography					268:281	column chromatography	261:281	column chromatography of DEAE Sepharose Fast Flow	261:309	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	0	27	dep	composition	74:84	arg1	the					70:72	the	70:72	the	70:72	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	1	28	theme	Fuzhuan	188:194	arg1	FBTPS					223:227	FBTPS	223:227	FBTPS	223:227	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	28	theme	Fuzhuan	188:194	arg1	polysaccharides					206:220	crude Fuzhuan brick tea polysaccharides	182:220	crude Fuzhuan brick tea polysaccharides (FBTPS)	182:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	0	29	theme	polysaccharides	21:35	arg1	fraction					9:16	Purified fraction	0:16	Purified fraction of polysaccharides from Fuzhuan brick tea	0:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	0	30	theme	anaerobic	122:130	arg1	fermentation					132:143	anaerobic fermentation	122:143	anaerobic fermentation	122:143	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	1	31	theme	brick	196:200	arg1	FBTPS					223:227	FBTPS	223:227	FBTPS	223:227	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	31	theme	brick	196:200	arg1	polysaccharides					206:220	crude Fuzhuan brick tea polysaccharides	182:220	crude Fuzhuan brick tea polysaccharides (FBTPS)	182:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	8	32	theme	acetic	1193:1198	arg1	acids					1225:1229	acetic, propionic and n-butyric acids	1193:1229	acetic, propionic and n-butyric acids	1193:1229	FBTPS-3 also significantly promoted the production of acetic, propionic and n-butyric acids.					
31446105	9	33	theme	starch	1343:1348	arg1	pathway					1332:1338	the metabolic pathway	1318:1338	the metabolic pathway of starch and sucrose	1318:1360	Based on the metagenomic sequencing, it was found that FBTPS-3 significantly enriched the metabolic pathway of starch and sucrose.					
31446105	2	34	theme	probiotic	340:348	arg1	effects					350:356	probiotic effects	340:356	probiotic effects	340:356	The chemical properties and probiotic effects of FBTPS-3 were evaluated by fermentation in vitro.					
31446105	1	35	theme	tea	202:204	arg1	FBTPS					223:227	FBTPS	223:227	FBTPS	223:227	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	35	theme	tea	202:204	arg1	polysaccharides					206:220	crude Fuzhuan brick tea polysaccharides	182:220	crude Fuzhuan brick tea polysaccharides (FBTPS)	182:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	0	36	theme	Fuzhuan	42:48	arg1	tea					56:58	Fuzhuan brick tea	42:58	Fuzhuan brick tea	42:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	10	37	theme	host	1478:1481	arg1	health					1483:1488	the host health	1474:1488	the host health	1474:1488	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	0	38	from	composition	74:84	arg1	fermentation					132:143	anaerobic fermentation	122:143	anaerobic fermentation	122:143	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	3	39	theme	FBTPS-3	435:441	arg1	effects					424:430	the effects	420:430	the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota	420:497	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	5	40	theme	carbohydrates	754:766	arg1	%					812:812	44.78 ± 2.85%	800:812	44.78 ± 2.85%	800:812	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	5	40	theme	carbohydrates	754:766	arg1	contents					742:749	The contents	738:749	The contents of carbohydrates and uronic acid in FBTPS-3	738:793	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	6	41	theme	carbohydrates	913:925	arg1	content					902:908	content	902:908	content	902:908	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	6	41	theme	carbohydrates	913:925	arg1	FBTPS-3					890:896	FBTPS-3	890:896	FBTPS-3	890:896	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	7	42	theme	relative	1035:1042	arg1	abundances					1044:1053	the relative abundances	1031:1053	the relative abundances of Bacteroides, Megasphaera and Prevotella	1031:1096	Furthermore, the relative abundances of Bacteroides, Megasphaera and Prevotella were significantly increased by FBTPS-3.					
31446105	1	43	from	polysaccharides	206:220	arg1	fraction					168:175	One purified fraction	155:175	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS)	155:228	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	1	43	from	polysaccharides	206:220	arg1	FBTPS-3					231:237	FBTPS-3	231:237	FBTPS-3	231:237	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	8	44	theme	acids	1225:1229	arg1	production					1179:1188	the production	1175:1188	the production of acetic, propionic and n-butyric acids	1175:1229	FBTPS-3 also significantly promoted the production of acetic, propionic and n-butyric acids.					
31446105	1	45	theme	Fast	301:304	arg1	Flow					306:309	DEAE Sepharose Fast Flow	286:309	DEAE Sepharose Fast Flow	286:309	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	5	46	theme	uronic	772:777	arg1	acid					779:782	uronic acid	772:782	uronic acid	772:782	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	0	47	theme	brick	50:54	arg1	tea					56:58	Fuzhuan brick tea	42:58	Fuzhuan brick tea	42:58	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	1	48	theme	Flow	306:309	arg1	chromatography					268:281	column chromatography	261:281	column chromatography of DEAE Sepharose Fast Flow	261:309	One purified fraction from crude Fuzhuan brick tea polysaccharides (FBTPS), FBTPS-3, was obtained through column chromatography of DEAE Sepharose Fast Flow.					
31446105	10	49	theme	gut	1513:1515	arg1	microbiota					1517:1526	the gut microbiota	1509:1526	the gut microbiota	1509:1526	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	9	50	theme	metagenomic	1245:1255	arg1	sequencing					1257:1266	the metagenomic sequencing	1241:1266	the metagenomic sequencing	1241:1266	Based on the metagenomic sequencing, it was found that FBTPS-3 significantly enriched the metabolic pathway of starch and sucrose.					
31446105	3	51	theme	microbiota	488:497	arg1	pathway					473:479	metabolic pathway	463:479	metabolic pathway	463:479	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	3	51	theme	microbiota	488:497	arg1	function					450:457	function	450:457	function	450:457	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	5	52	from	contents	742:749	arg1	FBTPS-3					787:793	FBTPS-3	787:793	FBTPS-3	787:793	The contents of carbohydrates and uronic acid in FBTPS-3 were 44.78 ± 2.85% and 40.4 ± 2.11%, respectively.					
31446105	4	53	with	heteropolysaccharide	583:602	arg1	weight					619:624	molecular weight	609:624	molecular weight of 741 kDa, which was mainly composed of Man	609:669	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	2	54	theme	chemical	316:323	arg1	properties					325:334	chemical properties	316:334	chemical properties	316:334	The chemical properties and probiotic effects of FBTPS-3 were evaluated by fermentation in vitro.					
31446105	4	55	theme	molecular	609:617	arg1	weight					619:624	molecular weight	609:624	molecular weight of 741 kDa, which was mainly composed of Man	609:669	The results showed that FBTPS-3 was an heteropolysaccharide with molecular weight of 741 kDa, which was mainly composed of Man, Rha, GalA, Gal and Ara in molar ratio of 8.7:15.5:42.2:19.7:13.9.					
31446105	3	56	theme	metabolic	463:471	arg1	pathway					473:479	metabolic pathway	463:479	metabolic pathway	463:479	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	8	57	theme	propionic	1201:1209	arg1	acids					1225:1229	acetic, propionic and n-butyric acids	1193:1229	acetic, propionic and n-butyric acids	1193:1229	FBTPS-3 also significantly promoted the production of acetic, propionic and n-butyric acids.					
31446105	0	58	from	metabolism	90:99	arg1	fermentation					132:143	anaerobic fermentation	122:143	anaerobic fermentation	122:143	Purified fraction of polysaccharides from Fuzhuan brick tea modulates the composition and metabolism of gut microbiota in anaerobic fermentation in vitro.					
31446105	2	59	theme	FBTPS-3	361:367	arg1	properties					325:334	chemical properties	316:334	chemical properties	316:334	The chemical properties and probiotic effects of FBTPS-3 were evaluated by fermentation in vitro.					
31446105	2	59	theme	FBTPS-3	361:367	arg1	effects					350:356	probiotic effects	340:356	probiotic effects	340:356	The chemical properties and probiotic effects of FBTPS-3 were evaluated by fermentation in vitro.					
31446105	10	60	theme	metabolic	1546:1554	arg1	functions					1556:1564	physiological metabolic functions	1532:1564	physiological metabolic functions	1532:1564	All the results suggest that FBTPS-3 is expected to be developed as functional ingredients or foods to improve the host health through regulating the gut microbiota and physiological metabolic functions.					
31446105	6	61	theme	FBTPS-3	890:896	arg1	weight					880:885	the molecular weight	866:885	the molecular weight of FBTPS-3 and content of carbohydrates	866:925	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
31446105	3	62	dep	function	450:457	arg1	the					446:448	the	446:448	the	446:448	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	3	63	from	effects	424:430	arg1	pathway					473:479	metabolic pathway	463:479	metabolic pathway	463:479	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	3	63	from	effects	424:430	arg1	function					450:457	function	450:457	function	450:457	Moreover, the effects of FBTPS-3 on the function and metabolic pathway of gut microbiota were investigated by metagenomic sequencing.					
31446105	6	64	theme	gut	1002:1004	arg1	microbiota					1006:1015	gut microbiota	1002:1015	gut microbiota	1002:1015	After fermentation, the molecular weight of FBTPS-3 and content of carbohydrates were significantly decreased, indicating that FBTPS-3 could be utilized by gut microbiota.					
29739851	2	0	theme	cell	719:722	arg1	line					724:727	a human breast fibroblast cell line	693:727	a human breast fibroblast cell line	693:727	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	0	theme	cell	719:722	arg1	CCD-1095Sk					730:739	CCD-1095Sk	730:739	CCD-1095Sk	730:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	5	1	theme	xyloside-primed	1447:1461	arg1	sulfate					1484:1490	the xyloside-primed chondroitin/dermatan sulfate	1443:1490	the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells	1443:1507	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	1	theme	xyloside-primed	1447:1461	arg1	complex					1518:1524	complex	1518:1524	complex	1518:1524	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	6	2	from	cells	1831:1835	arg1	sulfate					1812:1818	the cytotoxic chondroitin/dermatan sulfate	1777:1818	the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides	1777:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	6	2	from	cells	1831:1835	arg1	structure					1764:1772	the structure	1760:1772	the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides	1760:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	5	3	from	cells	1503:1507	arg1	sulfate					1484:1490	the xyloside-primed chondroitin/dermatan sulfate	1443:1490	the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells	1443:1507	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	3	from	cells	1503:1507	arg1	complex					1518:1524	complex	1518:1524	complex	1518:1524	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	4	4	theme	linkage	1341:1347	arg1	region					1349:1354	the linkage region	1337:1354	the linkage region	1337:1354	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	3	5	theme	novel	834:838	arg1	approach					849:856	a novel LC-MS/MS approach	832:856	a novel LC-MS/MS approach	832:856	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	2	6	contain	has	561:563	arg1	sulfate					497:503	xyloside-primed chondroitin/dermatan sulfate	460:503	xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70,	460:559	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	6	contain	has	561:563	arg2	effects					575:581	cytotoxic effects	565:581	cytotoxic effects	565:581	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	3	7	theme	dibutylamine	882:893	arg1	chromatography					907:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	4	8	theme	regions	1132:1138	arg1	characterization					1104:1119	detailed structural characterization	1084:1119	detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends	1084:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	3	9	theme	negative-mode	926:938	arg1	dissociation					964:975	negative-mode higher-energy collision dissociation	926:975	negative-mode higher-energy collision dissociation	926:975	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	2	10	theme	breast	701:706	arg1	line					724:727	a human breast fibroblast cell line	693:727	a human breast fibroblast cell line	693:727	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	10	theme	breast	701:706	arg1	CCD-1095Sk					730:739	CCD-1095Sk	730:739	CCD-1095Sk	730:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	3	11	with	combination	992:1002	arg1	studies					1021:1027	cell growth studies	1009:1027	cell growth studies	1009:1027	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	3	11	with	combination	992:1002	arg1	fingerprinting					1046:1059	disaccharide fingerprinting	1033:1059	disaccharide fingerprinting	1033:1059	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	4	12	theme	structural	1093:1102	arg1	characterization					1104:1119	detailed structural characterization	1084:1119	detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends	1084:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	6	13	theme	structural	1918:1927	arg1	characterization					1929:1944	structural characterization	1918:1944	structural characterization of glycosaminoglycans	1918:1966	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	4	14	theme	nonreducing	1416:1426	arg1	ends					1428:1431	the nonreducing ends	1412:1431	the nonreducing ends	1412:1431	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	1	15	theme	glycosaminoglycans	182:199	arg1	characterization					162:177	Structural characterization	151:177	Structural characterization of glycosaminoglycans	151:199	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	3	16	theme	cell	1009:1012	arg1	studies					1021:1027	cell growth studies	1009:1027	cell growth studies	1009:1027	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	4	17	theme	nonreducing	1172:1182	arg1	ends					1184:1187	nonreducing ends	1172:1187	nonreducing ends	1172:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	6	18	theme	approach	1905:1912	arg1	suitability					1877:1887	the suitability	1873:1887	the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans	1873:1966	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	4	19	theme	undescribed	1371:1381	arg1	methylation					1383:1393	previously undescribed methylation	1360:1393	previously undescribed methylation	1360:1393	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	5	20	theme	presence	1538:1545	arg1	terms					1529:1533	terms	1529:1533	terms of presence and distribution of iduronic acid	1529:1579	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	3	21	theme	collision	954:962	arg1	dissociation					964:975	negative-mode higher-energy collision dissociation	926:975	negative-mode higher-energy collision dissociation	926:975	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	6	22	theme	chondroitin/dermatan	1791:1810	arg1	sulfate					1812:1818	the cytotoxic chondroitin/dermatan sulfate	1777:1818	the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides	1777:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	4	23	from	cells	1285:1289	arg1	sulfate					1266:1272	xyloside-primed chondroitin/dermatan sulfate	1229:1272	xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1229:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	6	24	theme	LC-MS/MS	1896:1903	arg1	approach					1905:1912	the LC-MS/MS approach	1892:1912	the LC-MS/MS approach	1892:1912	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	3	25	theme	disaccharide	1033:1044	arg1	fingerprinting					1046:1059	disaccharide fingerprinting	1033:1059	disaccharide fingerprinting	1033:1059	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	4	26	theme	internal	1141:1148	arg1	oligosaccharides					1150:1165	internal oligosaccharides	1141:1165	internal oligosaccharides	1141:1165	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	0	27	theme	novel	123:127	arg1	modifications					136:148	novel glycan modifications	123:148	novel glycan modifications	123:148	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	2	28	theme	disaccharide	612:623	arg1	composition					625:635	disaccharide composition	612:635	disaccharide composition	612:635	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	4	29	theme	xyloside-primed	1229:1243	arg1	sulfate					1266:1272	xyloside-primed chondroitin/dermatan sulfate	1229:1272	xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1229:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	0	30	theme	LC-MS/MS	0:7	arg1	characterization					9:24	LC-MS/MS characterization	0:24	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties	0:88	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	5	31	theme	distribution	1551:1562	arg1	terms					1529:1533	terms	1529:1533	terms of presence and distribution of iduronic acid	1529:1579	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	2	32	theme	cell	543:546	arg1	HCC70					554:558	HCC70	554:558	HCC70	554:558	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	32	theme	cell	543:546	arg1	line					548:551	a human breast carcinoma cell line	518:551	a human breast carcinoma cell line	518:551	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	5	33	theme	iduronic	1567:1574	arg1	acid					1576:1579	iduronic acid	1567:1579	iduronic acid	1567:1579	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	2	34	theme	breast	526:531	arg1	HCC70					554:558	HCC70	554:558	HCC70	554:558	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	34	theme	breast	526:531	arg1	line					548:551	a human breast carcinoma cell line	518:551	a human breast carcinoma cell line	518:551	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	0	35	theme	glycosaminoglycans	45:62	arg1	characterization					9:24	LC-MS/MS characterization	0:24	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties	0:88	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	2	36	theme	cytotoxic	565:573	arg1	effects					575:581	cytotoxic effects	565:581	cytotoxic effects	565:581	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	0	37	theme	cytotoxic	69:77	arg1	properties					79:88	cytotoxic properties	69:88	cytotoxic properties	69:88	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	5	38	theme	iduronic	1639:1646	arg1	acid					1648:1651	iduronic acid	1639:1651	iduronic acid	1639:1651	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	39	theme	chondroitin/dermatan	1463:1482	arg1	sulfate					1484:1490	the xyloside-primed chondroitin/dermatan sulfate	1443:1490	the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells	1443:1507	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	39	theme	chondroitin/dermatan	1463:1482	arg1	complex					1518:1524	complex	1518:1524	complex	1518:1524	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	2	40	theme	chondroitin/dermatan	476:495	arg1	sulfate					497:503	xyloside-primed chondroitin/dermatan sulfate	460:503	xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70,	460:559	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	4	41	theme	HCC70	1279:1283	arg1	cells					1285:1289	HCC70 cells	1279:1289	HCC70 cells	1279:1289	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	2	42	theme	fibroblast	708:717	arg1	line					724:727	a human breast fibroblast cell line	693:727	a human breast fibroblast cell line	693:727	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	42	theme	fibroblast	708:717	arg1	CCD-1095Sk					730:739	CCD-1095Sk	730:739	CCD-1095Sk	730:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	6	43	dep	understanding	1746:1758	arg1	closer					1736:1741	closer	1736:1741	closer	1736:1741	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	5	44	from	complex	1518:1524	arg1	terms					1529:1533	terms	1529:1533	terms of presence and distribution of iduronic acid	1529:1579	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	4	45	theme	region	1349:1354	arg1	sulfation					1399:1407	sulfation	1399:1407	sulfation	1399:1407	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	45	theme	region	1349:1354	arg1	differences					1209:1219	differences	1209:1219	differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1209:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	45	theme	region	1349:1354	arg1	sialylation					1322:1332	sialylation	1322:1332	sialylation of the linkage region and previously undescribed methylation	1322:1393	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	5	46	theme	cytotoxic	1686:1694	arg1	effect					1696:1701	the cytotoxic effect	1682:1701	the cytotoxic effect	1682:1701	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	3	47	theme	LC-MS/MS	840:847	arg1	approach					849:856	a novel LC-MS/MS approach	832:856	a novel LC-MS/MS approach	832:856	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	3	48	theme	ion-pairing	895:905	arg1	chromatography					907:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	2	49	attach	derived	680:686	arg1	line					724:727	a human breast fibroblast cell line	693:727	a human breast fibroblast cell line	693:727	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	49	attach	derived	680:686	arg1	CCD-1095Sk					730:739	CCD-1095Sk	730:739	CCD-1095Sk	730:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	49	attach	derived	680:686	arg2	sulfate					672:678	nontoxic chondroitin/dermatan sulfate	642:678	nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk	642:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	1	50	theme	structure-function	254:271	arg1	relationships					273:285	structure-function relationships	254:285	structure-function relationships between glycosaminoglycans and the biomolecules with which they interact	254:358	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	5	51	from	terms	1529:1533	arg1	sulfate					1484:1490	the xyloside-primed chondroitin/dermatan sulfate	1443:1490	the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells	1443:1507	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	51	from	terms	1529:1533	arg1	complex					1518:1524	complex	1518:1524	complex	1518:1524	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	3	52	theme	reversed-phase	867:880	arg1	chromatography					907:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	reversed-phase dibutylamine ion-pairing chromatography	867:920	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	4	53	theme	linkage	1124:1130	arg1	regions					1132:1138	linkage regions	1124:1138	linkage regions	1124:1138	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	6	54	theme	glycosaminoglycans	1949:1966	arg1	characterization					1929:1944	structural characterization	1918:1944	structural characterization of glycosaminoglycans	1918:1966	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	3	55	theme	higher-energy	940:952	arg1	dissociation					964:975	negative-mode higher-energy collision dissociation	926:975	negative-mode higher-energy collision dissociation	926:975	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	1	56	theme	Structural	151:160	arg1	characterization					162:177	Structural characterization	151:177	Structural characterization of glycosaminoglycans	151:199	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	2	57	theme	human	695:699	arg1	line					724:727	a human breast fibroblast cell line	693:727	a human breast fibroblast cell line	693:727	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	57	theme	human	695:699	arg1	CCD-1095Sk					730:739	CCD-1095Sk	730:739	CCD-1095Sk	730:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	4	58	theme	ends	1428:1431	arg1	sulfation					1399:1407	sulfation	1399:1407	sulfation	1399:1407	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	58	theme	ends	1428:1431	arg1	differences					1209:1219	differences	1209:1219	differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1209:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	58	theme	ends	1428:1431	arg1	sialylation					1322:1332	sialylation	1322:1332	sialylation of the linkage region and previously undescribed methylation	1322:1393	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	6	59	theme	HCC70	1825:1829	arg1	cells					1831:1835	HCC70 cells	1825:1835	HCC70 cells primed on xylosides	1825:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	4	60	from	cells	1306:1310	arg1	sulfate					1266:1272	xyloside-primed chondroitin/dermatan sulfate	1229:1272	xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1229:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	61	theme	detailed	1084:1091	arg1	characterization					1104:1119	detailed structural characterization	1084:1119	detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends	1084:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	62	theme	methylation	1383:1393	arg1	sulfation					1399:1407	sulfation	1399:1407	sulfation	1399:1407	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	62	theme	methylation	1383:1393	arg1	differences					1209:1219	differences	1209:1219	differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1209:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	62	theme	methylation	1383:1393	arg1	sialylation					1322:1332	sialylation	1322:1332	sialylation of the linkage region and previously undescribed methylation	1322:1393	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	0	63	theme	structural	98:107	arg1	diversity					109:117	structural diversity	98:117	structural diversity	98:117	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	6	64	theme	sulfate	1812:1818	arg1	structure					1764:1772	the structure	1760:1772	the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides	1760:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	3	65	theme	growth	1014:1019	arg1	studies					1021:1027	cell growth studies	1009:1027	cell growth studies	1009:1027	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	6	66	theme	cytotoxic	1781:1789	arg1	sulfate					1812:1818	the cytotoxic chondroitin/dermatan sulfate	1777:1818	the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides	1777:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	4	67	theme	ends	1184:1187	arg1	characterization					1104:1119	detailed structural characterization	1084:1119	detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends	1084:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	3	68	used	used	981:984	arg2	we					819:820	we	819:820	we	819:820	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	0	69	theme	glycan	129:134	arg1	modifications					136:148	novel glycan modifications	123:148	novel glycan modifications	123:148	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	6	70	from	structure	1764:1772	arg1	cells					1831:1835	HCC70 cells	1825:1835	HCC70 cells primed on xylosides	1825:1855	Our data have moved us one step closer to understanding the structure of the cytotoxic chondroitin/dermatan sulfate from HCC70 cells primed on xylosides and demonstrate the suitability of the LC-MS/MS approach for structural characterization of glycosaminoglycans.					
29739851	5	71	theme	acid	1576:1579	arg1	distribution					1551:1562	distribution	1551:1562	distribution	1551:1562	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	5	71	theme	acid	1576:1579	arg1	presence					1538:1545	presence	1538:1545	presence	1538:1545	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	4	72	gly	sialylation	1322:1332	arg1	region					1349:1354	the linkage region	1337:1354	the linkage region	1337:1354	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	72	gly	sialylation	1322:1332	arg1	methylation					1383:1393	previously undescribed methylation	1360:1393	previously undescribed methylation	1360:1393	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	72	gly	sialylation	1322:1332	arg1	ends					1428:1431	the nonreducing ends	1412:1431	the nonreducing ends	1412:1431	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	4	73	theme	oligosaccharides	1150:1165	arg1	characterization					1104:1119	detailed structural characterization	1084:1119	detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends	1084:1187	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	2	74	theme	chondroitin/dermatan	651:670	arg1	sulfate					672:678	nontoxic chondroitin/dermatan sulfate	642:678	nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk	642:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	0	75	theme	xyloside-primed	29:43	arg1	glycosaminoglycans					45:62	xyloside-primed glycosaminoglycans	29:62	xyloside-primed glycosaminoglycans	29:62	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	4	76	theme	chondroitin/dermatan	1245:1264	arg1	sulfate					1266:1272	xyloside-primed chondroitin/dermatan sulfate	1229:1272	xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells	1229:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	3	77	theme	structural	769:778	arg1	requirements					780:791	the structural requirements	765:791	the structural requirements for the cytotoxic effect	765:816	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	2	78	theme	carcinoma	533:541	arg1	HCC70					554:558	HCC70	554:558	HCC70	554:558	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	78	theme	carcinoma	533:541	arg1	line					548:551	a human breast carcinoma cell line	518:551	a human breast carcinoma cell line	518:551	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	5	79	theme	glucuronic	1619:1628	arg1	acid					1630:1633	glucuronic acid	1619:1633	glucuronic acid	1619:1633	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	2	80	theme	human	520:524	arg1	HCC70					554:558	HCC70	554:558	HCC70	554:558	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	80	theme	human	520:524	arg1	line					548:551	a human breast carcinoma cell line	518:551	a human breast carcinoma cell line	518:551	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	81	theme	nontoxic	642:649	arg1	sulfate					672:678	nontoxic chondroitin/dermatan sulfate	642:678	nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk	642:739	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	3	82	theme	cytotoxic	801:809	arg1	effect					811:816	the cytotoxic effect	797:816	the cytotoxic effect	797:816	To further investigate the structural requirements for the cytotoxic effect, we developed a novel LC-MS/MS approach based on reversed-phase dibutylamine ion-pairing chromatography and negative-mode higher-energy collision dissociation and used it in combination with cell growth studies and disaccharide fingerprinting.					
29739851	1	83	dep	essential	228:236	arg1	determining					242:252	determining	242:252	determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact	242:358	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	1	83	dep	essential	228:236	arg1	gaining					368:374	gaining	368:374	gaining insight into the biosynthesis of glycosaminoglycans	368:426	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	0	84	with	characterization	9:24	arg1	properties					79:88	cytotoxic properties	69:88	cytotoxic properties	69:88	LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.					
29739851	1	85	theme	glycosaminoglycans	409:426	arg1	biosynthesis					393:404	the biosynthesis	389:404	the biosynthesis of glycosaminoglycans	389:426	Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans.					
29739851	4	86	theme	CCD-1095Sk	1295:1304	arg1	cells					1306:1310	CCD-1095Sk cells	1295:1310	CCD-1095Sk cells	1295:1310	This strategy enabled detailed structural characterization of linkage regions, internal oligosaccharides, and nonreducing ends, revealing not only differences between xyloside-primed chondroitin/dermatan sulfate from HCC70 cells and CCD-1095Sk cells, but also sialylation of the linkage region and previously undescribed methylation and sulfation of the nonreducing ends.					
29739851	5	87	theme	CCD-1095Sk	1596:1605	arg1	cells					1607:1611	CCD-1095Sk cells	1596:1611	CCD-1095Sk cells	1596:1611	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
29739851	2	88	attach	derived	505:511	arg1	HCC70					554:558	HCC70	554:558	HCC70	554:558	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	88	attach	derived	505:511	arg2	sulfate					497:503	xyloside-primed chondroitin/dermatan sulfate	460:503	xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70,	460:559	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	88	attach	derived	505:511	arg1	line					548:551	a human breast carcinoma cell line	518:551	a human breast carcinoma cell line	518:551	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	2	89	theme	xyloside-primed	460:474	arg1	sulfate					497:503	xyloside-primed chondroitin/dermatan sulfate	460:503	xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70,	460:559	We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell line, HCC70, has cytotoxic effects and shown that it differs in disaccharide composition from nontoxic chondroitin/dermatan sulfate derived from a human breast fibroblast cell line, CCD-1095Sk.					
29739851	5	90	theme	HCC70	1497:1501	arg1	cells					1503:1507	HCC70 cells	1497:1507	HCC70 cells	1497:1507	Although the xyloside-primed chondroitin/dermatan sulfate from HCC70 cells was less complex in terms of presence and distribution of iduronic acid than that from CCD-1095Sk cells, both glucuronic acid and iduronic acid appeared to be essential for the cytotoxic effect.					
31221330	0	0	theme	glucans	74:80	arg1	mixture					63:69	a complex mixture	53:69	a complex mixture of glucans and galactans	53:94	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	2	1	dep	named	599:603	arg1	CSGG					609:612	CSGG	609:612	CSGG	609:612	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	1	dep	named	599:603	arg1	polysaccharides					583:597	four neutral polysaccharides	570:597	four neutral polysaccharides named as (CSGG)	570:613	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	1	2	theme	NMR	344:346	arg1	analysis					361:368	NMR spectroscopy analysis	344:368	NMR spectroscopy analysis	344:368	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	3	theme	cell	163:166	arg1	polysaccharides					176:190	cell surface polysaccharides	163:190	cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants,	163:306	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	3	4	theme	pro-inflammatory	817:832	arg1	responses					841:849	pro-inflammatory immune responses	817:849	pro-inflammatory immune responses	817:849	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	3	5	theme	dendritic	786:794	arg1	cells					796:800	dendritic cells	786:800	dendritic cells	786:800	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	0	6	theme	galactans	86:94	arg1	mixture					63:69	a complex mixture	53:69	a complex mixture of glucans and galactans	53:94	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	1	7	theme	spectroscopy	348:359	arg1	analysis					361:368	NMR spectroscopy analysis	344:368	NMR spectroscopy analysis	344:368	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	2	8	theme	1 → 6	631:635	arg1	-glucan					637:643	β-(1 → 6)-glucan	628:643	β-(1 → 6)-glucan	628:643	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	3	9	theme	immune	834:839	arg1	responses					841:849	pro-inflammatory immune responses	817:849	pro-inflammatory immune responses	817:849	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	1	10	theme	gut	270:272	arg1	microbiota					274:283	the gut microbiota	266:283	the gut microbiota of breast-fed infants	266:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	5	11	dep	regulate	1188:1195	arg1	enhancing					1276:1284	enhancing	1276:1284	enhancing immunity	1276:1293	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	5	11	dep	regulate	1188:1195	arg1	inducing					1298:1305	inducing	1298:1305	inducing immune tolerance	1298:1322	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	0	12	attach	presents	24:31	arg1	surface					45:51	the cell surface	36:51	the cell surface	36:51	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	0	12	attach	presents	24:31	arg2	bifidum					16:22	Bifidobacterium bifidum	0:22	Bifidobacterium bifidum	0:22	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	1	13	theme	microbiota	274:283	arg1	member					256:261	an important member	243:261	an important member of the gut microbiota of breast-fed infants	243:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	13	theme	microbiota	274:283	arg1	PRI1					237:240	Bifidobacterium bifidum strain PRI1	206:240	Bifidobacterium bifidum strain PRI1	206:240	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	0	14	theme	different	101:109	arg1	properties					125:134	different immunological properties	101:134	different immunological properties	101:134	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	2	15	theme	β-	628:629	arg1	-glucan					637:643	β-(1 → 6)-glucan	628:643	β-(1 → 6)-glucan	628:643	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	1	16	theme	surface	168:174	arg1	polysaccharides					176:190	cell surface polysaccharides	163:190	cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants,	163:306	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	2	17	theme	main	489:492	arg1	PGβG					538:541	PGβG	538:541	PGβG	538:541	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	17	theme	main	489:492	arg1	groups					494:499	two main groups	485:499	two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch	485:712	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	17	theme	main	489:492	arg1	phospho-glycero-β-galactofuranan					504:535	a phospho-glycero-β-galactofuranan	502:535	a phospho-glycero-β-galactofuranan	502:535	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	17	theme	main	489:492	arg1	mixture					550:556	a mixture	548:556	a mixture composed of four neutral polysaccharides named as (CSGG)	548:613	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	18	theme	1 → 4	649:653	arg1	-galactan					655:663	β-(1 → 4)-galactan	646:663	β-(1 → 4)-galactan	646:663	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	1	19	theme	polysaccharides	176:190	arg1	structure					150:158	The chemical structure	137:158	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants,	137:306	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	4	20	theme	distinct	1026:1033	arg1	role					1035:1038	a distinct role	1024:1038	a distinct role	1024:1038	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	3	21	theme	interferon-γ	865:876	arg1	levels					878:883	interferon-γ levels	865:883	interferon-γ levels	865:883	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	5	22	theme	host	1201:1204	arg1	immunity					1206:1213	the host immunity	1197:1213	the host immunity	1197:1213	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	2	23	theme	β-	646:647	arg1	-galactan					655:663	β-(1 → 4)-galactan	646:663	β-(1 → 4)-galactan	646:663	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	3	24	theme	immunosuppressive	904:920	arg1	cells					935:939	CSGG induced immunosuppressive regulatory T cells	891:939	CSGG induced immunosuppressive regulatory T cells	891:939	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	1	25	theme	breast-fed	288:297	arg1	infants					299:305	breast-fed infants	288:305	breast-fed infants	288:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	3	26	theme	regulatory	922:931	arg1	cells					935:939	CSGG induced immunosuppressive regulatory T cells	891:939	CSGG induced immunosuppressive regulatory T cells	891:939	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	1	27	theme	Bifidobacterium	206:220	arg1	member					256:261	an important member	243:261	an important member of the gut microbiota of breast-fed infants	243:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	27	theme	Bifidobacterium	206:220	arg1	PRI1					237:240	Bifidobacterium bifidum strain PRI1	206:240	Bifidobacterium bifidum strain PRI1	206:240	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	2	28	theme	polysaccharides	441:455	arg1	mixture					430:436	a complex mixture	420:436	a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch	420:712	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	1	29	theme	infants	299:305	arg1	microbiota					274:283	the gut microbiota	266:283	the gut microbiota of breast-fed infants	266:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	0	30	theme	immunological	111:123	arg1	properties					125:134	different immunological properties	101:134	different immunological properties	101:134	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	4	31	theme	interaction	1114:1124	arg1	regulation					1081:1090	regulation	1081:1090	regulation of the host/bacterium interaction	1081:1124	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	0	32	theme	cell	40:43	arg1	surface					45:51	the cell surface	36:51	the cell surface	36:51	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	2	33	theme	complex	422:428	arg1	mixture					430:436	a complex mixture	420:436	a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch	420:712	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	3	34	theme	induced	896:902	arg1	cells					935:939	CSGG induced immunosuppressive regulatory T cells	891:939	CSGG induced immunosuppressive regulatory T cells	891:939	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	1	35	theme	strain	230:235	arg1	member					256:261	an important member	243:261	an important member of the gut microbiota of breast-fed infants	243:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	35	theme	strain	230:235	arg1	PRI1					237:240	Bifidobacterium bifidum strain PRI1	206:240	Bifidobacterium bifidum strain PRI1	206:240	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	2	36	theme	β-	666:667	arg1	-galactan					675:683	β-(1 → 6)-galactan	666:683	β-(1 → 6)-galactan	666:683	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	4	37	from	structure	1068:1076	arg1	regulation					1081:1090	regulation	1081:1090	regulation of the host/bacterium interaction	1081:1124	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	1	38	attach	isolated	192:199	arg1	member					256:261	an important member	243:261	an important member of the gut microbiota of breast-fed infants	243:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	38	attach	isolated	192:199	arg2	polysaccharides					176:190	cell surface polysaccharides	163:190	cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants,	163:306	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	38	attach	isolated	192:199	arg1	PRI1					237:240	Bifidobacterium bifidum strain PRI1	206:240	Bifidobacterium bifidum strain PRI1	206:240	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	2	39	dep	groups	494:499	arg1	PGβG					538:541	PGβG	538:541	PGβG	538:541	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	39	dep	groups	494:499	arg1	groups					494:499	two main groups	485:499	two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch	485:712	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	39	dep	groups	494:499	arg1	phospho-glycero-β-galactofuranan					504:535	a phospho-glycero-β-galactofuranan	502:535	a phospho-glycero-β-galactofuranan	502:535	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	39	dep	groups	494:499	arg1	mixture					550:556	a mixture	548:556	a mixture composed of four neutral polysaccharides named as (CSGG)	548:613	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	3	40	theme	T	933:933	arg1	cells					935:939	CSGG induced immunosuppressive regulatory T cells	891:939	CSGG induced immunosuppressive regulatory T cells	891:939	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	5	41	theme	immune	1307:1312	arg1	tolerance					1314:1322	immune tolerance	1307:1322	immune tolerance	1307:1322	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	2	42	theme	neutral	575:581	arg1	CSGG					609:612	CSGG	609:612	CSGG	609:612	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	2	42	theme	neutral	575:581	arg1	polysaccharides					583:597	four neutral polysaccharides	570:597	four neutral polysaccharides named as (CSGG)	570:613	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	4	43	contain	have	1019:1022	arg1	polysaccharides					1003:1017	bacterial polysaccharides	993:1017	bacterial polysaccharides	993:1017	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	4	43	contain	have	1019:1022	arg2	role					1035:1038	a distinct role	1024:1038	a distinct role	1024:1038	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	3	44	theme	immune	753:758	arg1	responses					760:768	different immune responses	743:768	different immune responses	743:768	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	4	45	theme	bacterial	993:1001	arg1	polysaccharides					1003:1017	bacterial polysaccharides	993:1017	bacterial polysaccharides	993:1017	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	4	46	theme	host/bacterium	1099:1112	arg1	interaction					1114:1124	the host/bacterium interaction	1095:1124	the host/bacterium interaction	1095:1124	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	5	47	theme	mixture	1260:1266	arg1	composition					1232:1242	the composition	1228:1242	the composition of this complex mixture	1228:1266	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	4	48	theme	chemical	1059:1066	arg1	structure					1068:1076	their chemical structure	1053:1076	their chemical structure in regulation of the host/bacterium interaction	1053:1124	These findings demonstrate that bacterial polysaccharides have a distinct role depending on their chemical structure in regulation of the host/bacterium interaction.					
31221330	1	49	dep	Bifidobacterium	206:220	arg1	bifidum					222:228	bifidum	222:228	bifidum	222:228	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	3	50	dep	induced	896:902	arg1	CSGG					891:894	CSGG	891:894	CSGG	891:894	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31221330	1	51	theme	chemical	141:148	arg1	structure					150:158	The chemical structure	137:158	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants,	137:306	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	52	theme	important	246:254	arg1	member					256:261	an important member	243:261	an important member of the gut microbiota of breast-fed infants	243:305	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	1	52	theme	important	246:254	arg1	PRI1					237:240	Bifidobacterium bifidum strain PRI1	206:240	Bifidobacterium bifidum strain PRI1	206:240	The chemical structure of cell surface polysaccharides isolated from Bifidobacterium bifidum strain PRI1, an important member of the gut microbiota of breast-fed infants, has been elucidated by chemical and NMR spectroscopy analysis.					
31221330	0	53	theme	complex	55:61	arg1	mixture					63:69	a complex mixture	53:69	a complex mixture of glucans and galactans	53:94	Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties.					
31221330	2	54	theme	1 → 6	669:673	arg1	-galactan					675:683	β-(1 → 6)-galactan	666:683	β-(1 → 6)-galactan	666:683	Results demonstrated that the bacterium produces a complex mixture of polysaccharides that could be classified in two main groups: a phospho-glycero-β-galactofuranan, PGβG, and a mixture composed of four neutral polysaccharides named as (CSGG), composed of β-(1 → 6)-glucan, β-(1 → 4)-galactan, β-(1 → 6)-galactan, β-galactofuranan and starch.					
31221330	5	55	theme	complex	1252:1258	arg1	mixture					1260:1266	this complex mixture	1247:1266	this complex mixture	1247:1266	Our findings suggest that polysaccharides may differentially regulate the host immunity depending on the composition of this complex mixture, either enhancing immunity or inducing immune tolerance.					
31221330	3	56	theme	different	743:751	arg1	responses					760:768	different immune responses	743:768	different immune responses	743:768	These two fractions exerted different immune responses when assayed on dendritic cells: PGβG enhanced pro-inflammatory immune responses by increasing interferon-γ levels while CSGG induced immunosuppressive regulatory T cells and interleukin-10.					
31166348	2	0	theme	same	444:447	arg1	compositions					464:475	the same monosaccharide compositions	440:475	the same monosaccharide compositions with different ratios	440:497	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	5	1	theme	MDA	1067:1069	arg1	production					1071:1080	MDA production	1067:1080	MDA production in healthy mouse liver homogenate	1067:1114	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	1	2	theme	fruit	141:145	arg1	MFP					163:165	MFP	163:165	MFP	163:165	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	1	2	theme	fruit	141:145	arg1	polysaccharide					147:160	Mulberry fruit polysaccharide	132:160	Mulberry fruit polysaccharide (MFP)	132:166	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	1	3	theme	water	209:213	arg1	method					226:231	a hot water extraction method	203:231	a hot water extraction method	203:231	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	4	4	theme	flat	912:915	arg1	sheets					917:922	flat sheets	912:922	flat sheets	912:922	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	1	5	theme	extraction	215:224	arg1	method					226:231	a hot water extraction method	203:231	a hot water extraction method	203:231	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	2	6	with	compositions	464:475	arg1	ratios					492:497	different ratios	482:497	different ratios	482:497	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	2	7	theme	different	482:490	arg1	ratios					492:497	different ratios	482:497	different ratios	482:497	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	1	8	theme	DEAE	315:318	arg1	chromatography					340:353	DEAE cellulose-52 column chromatography	315:353	DEAE cellulose-52 column chromatography	315:353	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	7	9	theme	organic	1359:1365	arg1	supplements					1372:1382	organic iron supplements	1359:1382	organic iron supplements	1359:1382	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	7	10	theme	antioxidant	1394:1404	arg1	activity					1406:1413	good antioxidant activity	1389:1413	good antioxidant activity	1389:1413	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	1	11	theme	cellulose-52	320:331	arg1	chromatography					340:353	DEAE cellulose-52 column chromatography	315:353	DEAE cellulose-52 column chromatography	315:353	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	4	12	theme	polysaccharide	737:750	arg1	mechanism					710:718	the chelate mechanism	698:718	the chelate mechanism of iron(iii) and polysaccharide	698:750	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	2	13	theme	Monosaccharide	356:369	arg1	analysis					371:378	Monosaccharide analysis	356:378	Monosaccharide analysis of MFP1, MFP2 and MFP3	356:401	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	0	14	theme	mori	8:11	arg1	chelates					36:43	Fructus mori L. polysaccharide-iron chelates	0:43	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core	0:95	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	1	15	theme	column	333:338	arg1	chromatography					340:353	DEAE cellulose-52 column chromatography	315:353	DEAE cellulose-52 column chromatography	315:353	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	6	16	theme	scavenging	1199:1208	arg1	ability					1210:1216	stronger superoxide radical scavenging ability	1171:1216	stronger superoxide radical scavenging ability	1171:1216	Also, the polysaccharide-iron(iii) chelates exhibited stronger superoxide radical scavenging ability than the polysaccharides.					
31166348	4	17	theme	iron	723:726	arg1	mechanism					710:718	the chelate mechanism	698:718	the chelate mechanism of iron(iii) and polysaccharide	698:750	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	5	18	theme	hydroxyl	1037:1044	arg1	radicals					1046:1053	hydroxyl radicals	1037:1053	hydroxyl radicals	1037:1053	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	0	19	theme	Fructus	0:6	arg1	chelates					36:43	Fructus mori L. polysaccharide-iron chelates	0:43	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core	0:95	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	6	20	theme	radical	1191:1197	arg1	ability					1210:1216	stronger superoxide radical scavenging ability	1171:1216	stronger superoxide radical scavenging ability	1171:1216	Also, the polysaccharide-iron(iii) chelates exhibited stronger superoxide radical scavenging ability than the polysaccharides.					
31166348	7	21	theme	good	1389:1392	arg1	activity					1406:1413	good antioxidant activity	1389:1413	good antioxidant activity	1389:1413	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	0	22	theme	polysaccharide-iron	16:34	arg1	chelates					36:43	Fructus mori L. polysaccharide-iron chelates	0:43	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core	0:95	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	1	23	theme	mulberry	238:245	arg1	fractions					262:270	mulberry polysaccharide fractions	238:270	mulberry polysaccharide fractions named MFP1, MFP2 and MFP3	238:296	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	0	24	theme	good	105:108	arg1	activity					122:129	good antioxidant activity	105:129	good antioxidant activity	105:129	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	2	25	contain	had	436:438	arg2	compositions					464:475	the same monosaccharide compositions	440:475	the same monosaccharide compositions with different ratios	440:497	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	2	25	contain	had	436:438	arg1	components					425:434	the three components	415:434	the three components	415:434	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	7	26	theme	iron	1367:1370	arg1	supplements					1372:1382	organic iron supplements	1359:1382	organic iron supplements	1359:1382	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	6	27	theme	superoxide	1180:1189	arg1	ability					1210:1216	stronger superoxide radical scavenging ability	1171:1216	stronger superoxide radical scavenging ability	1171:1216	Also, the polysaccharide-iron(iii) chelates exhibited stronger superoxide radical scavenging ability than the polysaccharides.					
31166348	2	28	theme	MFP2	389:392	arg1	analysis					371:378	Monosaccharide analysis	356:378	Monosaccharide analysis of MFP1, MFP2 and MFP3	356:401	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	0	29	theme	L.	13:14	arg1	chelates					36:43	Fructus mori L. polysaccharide-iron chelates	0:43	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core	0:95	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	1	30	theme	polysaccharide	247:260	arg1	fractions					262:270	mulberry polysaccharide fractions	238:270	mulberry polysaccharide fractions named MFP1, MFP2 and MFP3	238:296	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	5	31	theme	strong	996:1001	arg1	activity					1015:1022	strong antioxidant activity	996:1022	strong antioxidant activity	996:1022	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	5	32	from	production	1071:1080	arg1	homogenate					1105:1114	healthy mouse liver homogenate	1085:1114	healthy mouse liver homogenate	1085:1114	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	2	33	theme	galacturonic	504:515	arg1	acid					517:520	galacturonic acid	504:520	galacturonic acid	504:520	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	2	33	theme	galacturonic	504:515	arg1	component					550:558	the main monosaccharide component	526:558	the main monosaccharide component	526:558	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	5	34	theme	antioxidant	1003:1013	arg1	activity					1015:1022	strong antioxidant activity	996:1022	strong antioxidant activity	996:1022	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	3	35	theme	weight	571:576	arg1	measurements					578:589	Molecular weight measurements	561:589	Molecular weight measurements	561:589	Molecular weight measurements showed that MFP1 and MFP2 are heteropolysaccharides and MFP3 is a homogeneous polysaccharide.					
31166348	2	36	theme	MFP1	383:386	arg1	analysis					371:378	Monosaccharide analysis	356:378	Monosaccharide analysis of MFP1, MFP2 and MFP3	356:401	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	5	37	theme	healthy	1085:1091	arg1	homogenate					1105:1114	healthy mouse liver homogenate	1085:1114	healthy mouse liver homogenate	1085:1114	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	0	38	theme	antioxidant	110:120	arg1	activity					122:129	good antioxidant activity	105:129	good antioxidant activity	105:129	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	2	39	theme	MFP3	398:401	arg1	analysis					371:378	Monosaccharide analysis	356:378	Monosaccharide analysis of MFP1, MFP2 and MFP3	356:401	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	5	40	theme	mouse	1093:1097	arg1	homogenate					1105:1114	healthy mouse liver homogenate	1085:1114	healthy mouse liver homogenate	1085:1114	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	7	41	dep	Morus	1304:1308	arg1	L.					1315:1316	Morus alba L.	1304:1316	Morus alba L.	1304:1316	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	4	42	theme	hydroxyl	843:850	arg1	groups					865:870	hydroxyl and carboxyl groups	843:870	groups	865:870	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	5	43	theme	liver	1099:1103	arg1	homogenate					1105:1114	healthy mouse liver homogenate	1085:1114	healthy mouse liver homogenate	1085:1114	Furthermore, the polysaccharides showed strong antioxidant activity to eliminate hydroxyl radicals and inhibit MDA production in healthy mouse liver homogenate.					
31166348	2	44	theme	monosaccharide	535:548	arg1	acid					517:520	galacturonic acid	504:520	galacturonic acid	504:520	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	2	44	theme	monosaccharide	535:548	arg1	component					550:558	the main monosaccharide component	526:558	the main monosaccharide component	526:558	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	3	45	theme	Molecular	561:569	arg1	measurements					578:589	Molecular weight measurements	561:589	Molecular weight measurements	561:589	Molecular weight measurements showed that MFP1 and MFP2 are heteropolysaccharides and MFP3 is a homogeneous polysaccharide.					
31166348	4	46	theme	morphology	889:898	arg1	change					900:905	a morphology change	887:905	a morphology change from flat sheets to rods	887:930	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	2	47	theme	main	530:533	arg1	acid					517:520	galacturonic acid	504:520	galacturonic acid	504:520	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	2	47	theme	main	530:533	arg1	component					550:558	the main monosaccharide component	526:558	the main monosaccharide component	526:558	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	3	48	theme	homogeneous	657:667	arg1	polysaccharide					669:682	a homogeneous polysaccharide	655:682	a homogeneous polysaccharide	655:682	Molecular weight measurements showed that MFP1 and MFP2 are heteropolysaccharides and MFP3 is a homogeneous polysaccharide.					
31166348	3	48	theme	homogeneous	657:667	arg1	MFP3					647:650	MFP3	647:650	MFP3	647:650	Molecular weight measurements showed that MFP1 and MFP2 are heteropolysaccharides and MFP3 is a homogeneous polysaccharide.					
31166348	1	49	dep	Morus	186:190	arg1	L.					197:198	Morus alba L.	186:198	Morus alba L.	186:198	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	6	50	theme	stronger	1171:1178	arg1	ability					1210:1216	stronger superoxide radical scavenging ability	1171:1216	stronger superoxide radical scavenging ability	1171:1216	Also, the polysaccharide-iron(iii) chelates exhibited stronger superoxide radical scavenging ability than the polysaccharides.					
31166348	4	51	from	sheets	917:922	arg1	change					900:905	a morphology change	887:905	a morphology change from flat sheets to rods	887:930	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	7	52	attach	derived	1291:1297	arg2	candidates					1332:1341	promising candidates	1322:1341	promising candidates for fabricating organic iron supplements with good antioxidant activity	1322:1413	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	7	52	attach	derived	1291:1297	arg2	polysaccharides					1275:1289	the polysaccharides	1271:1289	the polysaccharides derived from Morus alba L.	1271:1316	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	7	52	attach	derived	1291:1297	arg1	Morus					1304:1308	Morus	1304:1308	Morus	1304:1308	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	7	53	theme	promising	1322:1330	arg1	polysaccharides					1275:1289	the polysaccharides	1271:1289	the polysaccharides derived from Morus alba L.	1271:1316	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	7	53	theme	promising	1322:1330	arg1	candidates					1332:1341	promising candidates	1322:1341	promising candidates for fabricating organic iron supplements with good antioxidant activity	1322:1413	These results suggest that the polysaccharides derived from Morus alba L. are promising candidates for fabricating organic iron supplements with good antioxidant activity.					
31166348	0	54	with	self-embedding	55:68	arg1	iron					75:78	iron(iii)	75:83	iron(iii)	75:83	Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity.					
31166348	4	55	theme	chelate	702:708	arg1	mechanism					710:718	the chelate mechanism	698:718	the chelate mechanism of iron(iii) and polysaccharide	698:750	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	4	56	theme	carboxyl	856:863	arg1	groups					865:870	hydroxyl and carboxyl groups	843:870	groups	865:870	In addition, the chelate mechanism of iron(iii) and polysaccharide is proposed in which iron(iii) as a core is enwrapped by the polysaccharide as a ligand by hydroxyl and carboxyl groups, which induces a morphology change from flat sheets to rods and increases the size.					
31166348	2	57	theme	monosaccharide	449:462	arg1	compositions					464:475	the same monosaccharide compositions	440:475	the same monosaccharide compositions with different ratios	440:497	Monosaccharide analysis of MFP1, MFP2 and MFP3 showed that the three components had the same monosaccharide compositions with different ratios, and galacturonic acid was the main monosaccharide component.					
31166348	6	58	theme	polysaccharide-iron	1127:1145	arg1	chelates					1152:1159	the polysaccharide-iron(iii) chelates	1123:1159	the polysaccharide-iron(iii) chelates	1123:1159	Also, the polysaccharide-iron(iii) chelates exhibited stronger superoxide radical scavenging ability than the polysaccharides.					
31166348	1	59	theme	Mulberry	132:139	arg1	MFP					163:165	MFP	163:165	MFP	163:165	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	1	59	theme	Mulberry	132:139	arg1	polysaccharide					147:160	Mulberry fruit polysaccharide	132:160	Mulberry fruit polysaccharide (MFP)	132:166	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31166348	1	60	theme	hot	205:207	arg1	method					226:231	a hot water extraction method	203:231	a hot water extraction method	203:231	Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.					
31437508	7	0	theme	better	1225:1230	arg1	effect					1251:1256	a better immunity enhancing effect	1223:1256	a better immunity enhancing effect	1223:1256	However, FVPH had a better immunity enhancing effect compared with FVPU.					
31437508	3	1	theme	lower	758:762	arg1	polysaccharide					764:777	lower polysaccharide and polyphenol contents	758:801	polysaccharide	764:777	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	1	2	theme	activity	559:566	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	5	3	theme	superoxide	1115:1124	arg1	radical					1132:1138	superoxide anion radical	1115:1138	superoxide anion radical	1115:1138	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	8	4	from	effect	1326:1331	arg1	structure					1360:1368	the structure	1356:1368	the structure of polysaccharides	1356:1387	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	4	5	theme	molecular	940:948	arg1	weight					950:955	low molecular weight	936:955	low molecular weight polysaccharide components of FVPU	936:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	3	6	theme	protein	713:719	arg1	content					721:727	protein content	713:727	protein content	713:727	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	1	7	theme	primary	437:443	arg1	compositions					445:456	primary compositions	437:456	primary compositions	437:456	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	8	theme	ultrasound-assisted	189:207	arg1	UAE					221:223	UAE	221:223	UAE	221:223	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	8	theme	ultrasound-assisted	189:207	arg1	extraction					209:218	ultrasound-assisted extraction	189:218	ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs)	189:271	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	0	9	theme	velutipes	124:132	arg1	activity					101:108	bidirectional immunomodulatory activity	70:108	bidirectional immunomodulatory activity	70:108	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	0	9	theme	velutipes	124:132	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	0	10	from	Effects	0:6	arg1	activity					101:108	bidirectional immunomodulatory activity	70:108	bidirectional immunomodulatory activity	70:108	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	0	10	from	Effects	0:6	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	1	11	theme	compositions	445:456	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	9	12	theme	functional	1586:1595	arg1	foods					1597:1601	functional foods	1586:1601	functional foods	1586:1601	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	0	13	theme	Flammulina	113:122	arg1	velutipes					124:132	Flammulina velutipes	113:132	Flammulina velutipes	113:132	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	1	14	theme	water	380:384	arg1	extraction					386:395	hot water extraction	376:395	hot water extraction (FVPH)	376:402	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	14	theme	water	380:384	arg1	FVPH					398:401	FVPH	398:401	FVPH	398:401	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	7	15	theme	immunity	1232:1239	arg1	effect					1251:1256	a better immunity enhancing effect	1223:1256	a better immunity enhancing effect	1223:1256	However, FVPH had a better immunity enhancing effect compared with FVPU.					
31437508	0	16	dep	polysaccharide	134:147	arg1	Effects					0:6	Effects	0:6	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes	0:132	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	8	17	theme	UAE	1422:1424	arg1	process					1411:1417	the extraction process	1396:1417	the extraction process of UAE	1396:1424	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	5	18	theme	anion	1126:1130	arg1	radical					1132:1138	superoxide anion radical	1115:1138	superoxide anion radical	1115:1138	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	2	19	theme	FVPs	673:676	arg1	properties					659:668	the above properties	649:668	the above properties of FVPs	649:676	Results indicated that UAE changed the above properties of FVPs.					
31437508	1	20	theme	surface	459:465	arg1	microstructure					467:480	surface microstructure	459:480	surface microstructure	459:480	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	8	21	theme	polysaccharides	1373:1387	arg1	structure					1360:1368	the structure	1356:1368	the structure of polysaccharides	1356:1387	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	9	22	theme	environmentally	1482:1496	arg1	method					1507:1512	an efficient and environmentally friendly method	1465:1512	an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals	1465:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	9	22	theme	environmentally	1482:1496	arg1	UAE					1457:1459	UAE	1457:1459	UAE	1457:1459	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	3	23	theme	uronic	734:739	arg1	content					746:752	uronic acid content	734:752	uronic acid content	734:752	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	1	24	theme	microstructure	467:480	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	9	25	theme	foods	1597:1601	arg1	development					1571:1581	the development	1567:1581	the development of functional foods or nutraceuticals	1567:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	1	26	theme	bidirectional	573:585	arg1	activity					604:611	bidirectional immunomodulatory activity	573:611	bidirectional immunomodulatory activity	573:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	3	27	theme	acid	741:744	arg1	content					746:752	uronic acid content	734:752	uronic acid content	734:752	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	4	28	theme	helix	887:891	arg1	structure					893:901	the triple helix structure	876:901	the triple helix structure	876:901	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	7	29	theme	enhancing	1241:1249	arg1	effect					1251:1256	a better immunity enhancing effect	1223:1256	a better immunity enhancing effect	1223:1256	However, FVPH had a better immunity enhancing effect compared with FVPU.					
31437508	1	30	theme	immunomodulatory	587:602	arg1	activity					604:611	bidirectional immunomodulatory activity	573:611	bidirectional immunomodulatory activity	573:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	9	31	theme	efficient	1468:1476	arg1	method					1507:1512	an efficient and environmentally friendly method	1465:1512	an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals	1465:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	9	31	theme	efficient	1468:1476	arg1	UAE					1457:1459	UAE	1457:1459	UAE	1457:1459	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	9	32	from	velutipes	1553:1561	arg1	polysaccharides					1529:1543	new polysaccharides	1525:1543	new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals	1525:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	2	33	theme	above	653:657	arg1	properties					659:668	the above properties	649:668	the above properties of FVPs	649:676	Results indicated that UAE changed the above properties of FVPs.					
31437508	1	34	theme	helix-coil	483:492	arg1	structure					505:513	helix-coil transition structure	483:513	helix-coil transition structure	483:513	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	6	35	theme	inflammation	1172:1183	arg1	inhibitor					1159:1167	a better inhibitor	1150:1167	a better inhibitor of inflammation	1150:1183	FVPU was a better inhibitor of inflammation compared with FVPH.					
31437508	6	35	theme	inflammation	1172:1183	arg1	FVPU					1141:1144	FVPU	1141:1144	FVPU	1141:1144	FVPU was a better inhibitor of inflammation compared with FVPH.					
31437508	1	36	theme	activity	604:611	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	0	37	theme	extraction	31:40	arg1	Effects					0:6	Effects	0:6	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes	0:132	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	3	38	theme	polyphenol	783:792	arg1	contents					794:801	lower polysaccharide and polyphenol contents	758:801	contents	794:801	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	4	39	theme	weight	950:955	arg1	components					972:981	low molecular weight polysaccharide components	936:981	low molecular weight polysaccharide components of FVPU	936:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	1	40	theme	transition	494:503	arg1	structure					505:513	helix-coil transition structure	483:513	helix-coil transition structure	483:513	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	8	41	theme	waves	1347:1351	arg1	effect					1326:1331	the cavitation effect	1311:1331	the cavitation effect of ultrasonic waves on the structure of polysaccharides	1311:1387	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	5	42	from	activities	1065:1074	arg1	radical					1084:1090	DPPH radical	1079:1090	DPPH radical	1079:1090	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	5	42	from	activities	1065:1074	arg1	radical					1102:1108	hydroxyl radical	1093:1108	hydroxyl radical	1093:1108	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	5	42	from	activities	1065:1074	arg1	radical					1132:1138	superoxide anion radical	1115:1138	superoxide anion radical	1115:1138	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	9	43	theme	friendly	1498:1505	arg1	method					1507:1512	an efficient and environmentally friendly method	1465:1512	an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals	1465:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	9	43	theme	friendly	1498:1505	arg1	UAE					1457:1459	UAE	1457:1459	UAE	1457:1459	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	0	44	theme	ultrasound-assisted	11:29	arg1	extraction					31:40	ultrasound-assisted extraction	11:40	ultrasound-assisted extraction	11:40	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	1	45	theme	ultrasound-assisted	316:334	arg1	FVPU					348:351	FVPU	348:351	FVPU	348:351	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	45	theme	ultrasound-assisted	316:334	arg1	extraction					336:345	ultrasound-assisted extraction	316:345	ultrasound-assisted extraction (FVPU)	316:352	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	3	46	located	observed	808:815	arg2	content					746:752	uronic acid content	734:752	uronic acid content	734:752	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	3	46	located	observed	808:815	arg1	FVPU					820:823	FVPU	820:823	FVPU	820:823	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	3	46	located	observed	808:815	arg2	yield					706:710	higher yield	699:710	higher yield	699:710	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	3	46	located	observed	808:815	arg2	content					721:727	protein content	713:727	protein content	713:727	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	3	46	located	observed	808:815	arg2	polysaccharide					764:777	lower polysaccharide and polyphenol contents	758:801	polysaccharide	764:777	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	5	47	theme	hydroxyl	1093:1100	arg1	radical					1102:1108	hydroxyl radical	1093:1108	hydroxyl radical	1093:1108	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	8	48	theme	extraction	1400:1409	arg1	process					1411:1417	the extraction process	1396:1417	the extraction process of UAE	1396:1424	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	5	49	from	power	1044:1048	arg1	radical					1084:1090	DPPH radical	1079:1090	DPPH radical	1079:1090	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	5	49	from	power	1044:1048	arg1	radical					1102:1108	hydroxyl radical	1093:1108	hydroxyl radical	1093:1108	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	5	49	from	power	1044:1048	arg1	radical					1132:1138	superoxide anion radical	1115:1138	superoxide anion radical	1115:1138	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	1	50	theme	Flammulina	229:238	arg1	FVPs					267:270	FVPs	267:270	FVPs	267:270	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	50	theme	Flammulina	229:238	arg1	polysaccharides					250:264	Flammulina velutipes polysaccharides	229:264	Flammulina velutipes polysaccharides (FVPs)	229:271	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	0	51	theme	antioxidant	45:55	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	9	52	theme	new	1525:1527	arg1	polysaccharides					1529:1543	new polysaccharides	1525:1543	new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals	1525:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	4	53	theme	components	972:981	arg1	proportion					922:931	the proportion	918:931	the proportion of low molecular weight polysaccharide components of FVPU	918:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	4	54	theme	surface	842:848	arg1	microstructure					850:863	the surface microstructure	838:863	the surface microstructure	838:863	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	1	55	theme	velutipes	240:248	arg1	FVPs					267:270	FVPs	267:270	FVPs	267:270	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	55	theme	velutipes	240:248	arg1	polysaccharides					250:264	Flammulina velutipes polysaccharides	229:264	Flammulina velutipes polysaccharides (FVPs)	229:271	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	4	56	theme	polysaccharide	957:970	arg1	components					972:981	low molecular weight polysaccharide components	936:981	low molecular weight polysaccharide components of FVPU	936:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	1	57	theme	structure	505:513	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	4	58	theme	FVPU	986:989	arg1	components					972:981	low molecular weight polysaccharide components	936:981	low molecular weight polysaccharide components of FVPU	936:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	5	59	theme	scavenging	1054:1063	arg1	activities					1065:1074	scavenging activities	1054:1074	scavenging activities	1054:1074	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	4	60	theme	triple	880:885	arg1	structure					893:901	the triple helix structure	876:901	the triple helix structure	876:901	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	6	61	theme	better	1152:1157	arg1	inhibitor					1159:1167	a better inhibitor	1150:1167	a better inhibitor of inflammation	1150:1183	FVPU was a better inhibitor of inflammation compared with FVPH.					
31437508	6	61	theme	better	1152:1157	arg1	FVPU					1141:1144	FVPU	1141:1144	FVPU	1141:1144	FVPU was a better inhibitor of inflammation compared with FVPH.					
31437508	1	62	theme	hot	376:378	arg1	extraction					386:395	hot water extraction	376:395	hot water extraction (FVPH)	376:402	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	62	theme	hot	376:378	arg1	FVPH					398:401	FVPH	398:401	FVPH	398:401	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	63	theme	molecular	516:524	arg1	distribution					533:544	molecular weight distribution	516:544	molecular weight distribution	516:544	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	9	64	theme	nutraceuticals	1606:1619	arg1	development					1571:1581	the development	1567:1581	the development of functional foods or nutraceuticals	1567:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	4	65	theme	low	936:938	arg1	weight					950:955	low molecular weight	936:955	low molecular weight polysaccharide components of FVPU	936:989	UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU.					
31437508	5	66	theme	DPPH	1079:1082	arg1	radical					1084:1090	DPPH radical	1079:1090	DPPH radical	1079:1090	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	1	67	theme	weight	526:531	arg1	distribution					533:544	molecular weight distribution	516:544	molecular weight distribution	516:544	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	68	from	extraction	209:218	arg1	FVPs					267:270	FVPs	267:270	FVPs	267:270	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	68	from	extraction	209:218	arg1	polysaccharides					250:264	Flammulina velutipes polysaccharides	229:264	Flammulina velutipes polysaccharides (FVPs)	229:271	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	7	69	contain	had	1219:1221	arg2	effect					1251:1256	a better immunity enhancing effect	1223:1256	a better immunity enhancing effect	1223:1256	However, FVPH had a better immunity enhancing effect compared with FVPU.					
31437508	7	69	contain	had	1219:1221	arg1	FVPH					1214:1217	FVPH	1214:1217	FVPH	1214:1217	However, FVPH had a better immunity enhancing effect compared with FVPU.					
31437508	1	70	theme	yield	430:434	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	1	71	theme	distribution	533:544	arg1	terms					421:425	terms	421:425	terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity	421:611	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31437508	8	72	theme	cavitation	1315:1324	arg1	effect					1326:1331	the cavitation effect	1311:1331	the cavitation effect of ultrasonic waves on the structure of polysaccharides	1311:1387	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	0	73	theme	immunomodulatory	84:99	arg1	activity					101:108	bidirectional immunomodulatory activity	70:108	bidirectional immunomodulatory activity	70:108	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	3	74	theme	higher	699:704	arg1	yield					706:710	higher yield	699:710	higher yield	699:710	Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU.					
31437508	5	75	theme	stronger	1026:1033	arg1	power					1044:1048	a stronger reducing power	1024:1048	a stronger reducing power	1024:1048	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	9	76	theme	F.	1550:1551	arg1	velutipes					1553:1561	F. velutipes	1550:1561	F. velutipes for the development of functional foods or nutraceuticals	1550:1619	These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.					
31437508	8	77	theme	ultrasonic	1336:1345	arg1	waves					1347:1351	ultrasonic waves	1336:1351	ultrasonic waves	1336:1351	These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE.					
31437508	0	78	theme	bidirectional	70:82	arg1	activity					101:108	bidirectional immunomodulatory activity	70:108	bidirectional immunomodulatory activity	70:108	Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide.					
31437508	5	79	theme	reducing	1035:1042	arg1	power					1044:1048	a stronger reducing power	1024:1048	a stronger reducing power	1024:1048	Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical.					
31437508	1	80	theme	antioxidant	547:557	arg1	activity					559:566	antioxidant activity	547:566	antioxidant activity	547:566	In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity.					
31173827	6	0	dep	endo-1,4-β-xylanase	907:925	arg1	i					904:904	i	904:904	i	904:904	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	0	1	theme	coracana	78:85	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	0	2	from	hydrolysis	10:19	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	1	3	theme	enzymatic	177:185	arg1	saccharification					187:202	enzymatic saccharification	177:202	enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose	177:293	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	6	4	theme	hemicellulose	838:850	arg1	saccharification					852:867	hemicellulose saccharification	838:867	hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5	838:1009	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	5	theme	pretreated	884:893	arg1	FMS					895:897	the above pretreated FMS	874:897	the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5	874:1009	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	6	from	37 °C	989:993	arg1	FMS					895:897	the above pretreated FMS	874:897	the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5	874:1009	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	4	7	theme	FMS	730:732	arg1	%					712:712	69.3%	708:712	69.3% of raw powdered FMS	708:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	4	7	theme	FMS	730:732	arg1	FMS					730:732	raw powdered FMS	717:732	raw powdered FMS	717:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	6	8	theme	pretreated	1060:1069	arg1	biomass					1071:1077	70 mg/g pretreated biomass	1052:1077	70 mg/g pretreated biomass	1052:1077	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	9	theme	Box-Behnken	1014:1024	arg1	design					1026:1031	Box-Behnken design	1014:1031	Box-Behnken design	1014:1031	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	0	10	theme	recombinant	97:107	arg1	endo-1,4-β-xylanase					109:127	recombinant endo-1,4-β-xylanase	97:127	recombinant endo-1,4-β-xylanase	97:127	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	6	11	dep	37 °C	989:993	arg1	both					996:999	both	996:999	both	996:999	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	12	theme	biomass	1071:1077	arg1	TRS					1045:1047	the TRS	1041:1047	the TRS of 70 mg/g pretreated biomass	1041:1077	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	7	13	theme	percentage	1084:1093	arg1	conversion					1095:1104	The percentage conversion	1080:1104	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A	1080:1147	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A was 24.7%.					
31173827	7	13	theme	percentage	1084:1093	arg1	%					1157:1157	24.7%	1153:1157	24.7%	1153:1157	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A was 24.7%.					
31173827	3	14	theme	recombinant	409:419	arg1	active					461:466	active	461:466	active	461:466	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	14	theme	recombinant	409:419	arg1	CtXyn11A					442:449	CtXyn11A	442:449	CtXyn11A	442:449	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	14	theme	recombinant	409:419	arg1	endo-1,4-β-xylanase					421:439	The recombinant endo-1,4-β-xylanase	405:439	The recombinant endo-1,4-β-xylanase (CtXyn11A)	405:450	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	5	15	theme	biomass	769:775	arg1	yield					777:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	5	15	theme	biomass	769:775	arg1	biomass					800:806	biomass	800:806	biomass	800:806	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	6	16	theme	saccharification	852:867	arg1	optimization					822:833	The two-step optimization	809:833	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design	809:1031	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	17	from	FMS	895:897	arg1	optimization					822:833	The two-step optimization	809:833	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design	809:1031	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	17	from	FMS	895:897	arg1	saccharification					852:867	hemicellulose saccharification	838:867	hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5	838:1009	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	1	18	theme	hemicellulose	207:219	arg1	part					221:224	hemicellulose part	207:224	hemicellulose part of the pretreated Finger millet straw (FMS)	207:268	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	0	19	theme	Enzymatic	0:8	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.	0:153	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	1	20	theme	xylose	288:293	arg1	production					274:283	production	274:283	production of xylose	274:293	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	2	21	theme	carbohydrate	317:328	arg1	composition					330:340	the carbohydrate composition	313:340	the carbohydrate composition of FMS	313:347	The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments.					
31173827	1	22	theme	part	221:224	arg1	saccharification					187:202	enzymatic saccharification	177:202	enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose	177:293	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	0	23	theme	hemicellulose	24:36	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.	0:153	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	4	24	from	%	712:712	arg1	content					695:701	the holocellulose content	677:701	the holocellulose content from 69.3% of raw powdered FMS	677:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	4	25	theme	powdered	721:728	arg1	FMS					730:732	raw powdered FMS	717:732	raw powdered FMS	717:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	3	26	theme	32 mg/g	609:615	arg1	biomass					628:634	32 mg/g pretreated biomass	609:634	32 mg/g pretreated biomass	609:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	4	27	theme	raw	717:719	arg1	FMS					730:732	raw powdered FMS	717:732	raw powdered FMS	717:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	6	28	theme	70 mg/g	1052:1058	arg1	biomass					1071:1077	70 mg/g pretreated biomass	1052:1077	70 mg/g pretreated biomass	1052:1077	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	0	29	theme	pretreated	43:52	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	3	30	theme	pretreated	617:626	arg1	biomass					628:634	32 mg/g pretreated biomass	609:634	32 mg/g pretreated biomass	609:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	31	theme	total	573:577	arg1	yield					594:598	a total reducing sugar yield	571:598	a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass	571:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	31	theme	total	573:577	arg1	TRS					601:603	TRS	601:603	TRS	601:603	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	1	32	theme	pretreated	233:242	arg1	FMS					265:267	FMS	265:267	FMS	265:267	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	1	32	theme	pretreated	233:242	arg1	straw					258:262	the pretreated Finger millet straw	229:262	the pretreated Finger millet straw (FMS)	229:268	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	7	33	theme	xylan	1109:1113	arg1	conversion					1095:1104	The percentage conversion	1080:1104	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A	1080:1147	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A was 24.7%.					
31173827	7	33	theme	xylan	1109:1113	arg1	%					1157:1157	24.7%	1153:1157	24.7%	1153:1157	The percentage conversion of xylan to xylose by CtXyn11A and BoGH43A was 24.7%.					
31173827	6	34	from	55 °C	941:945	arg1	exo-1,4-β-xylosidase					955:974	ii) exo-1,4-β-xylosidase	951:974	ii) exo-1,4-β-xylosidase (BoGH43A)	951:984	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	34	from	55 °C	941:945	arg1	CtXyn11A					928:935	CtXyn11A	928:935	CtXyn11A	928:935	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	34	from	55 °C	941:945	arg1	endo-1,4-β-xylanase					907:925	endo-1,4-β-xylanase	907:925	i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C	904:945	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	34	from	55 °C	941:945	arg1	BoGH43A					977:983	BoGH43A	977:983	BoGH43A	977:983	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	3	35	theme	reducing	579:586	arg1	yield					594:598	a total reducing sugar yield	571:598	a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass	571:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	35	theme	reducing	579:586	arg1	TRS					601:603	TRS	601:603	TRS	601:603	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	1	36	theme	Finger	244:249	arg1	FMS					265:267	FMS	265:267	FMS	265:267	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	1	36	theme	Finger	244:249	arg1	straw					258:262	the pretreated Finger millet straw	229:262	the pretreated Finger millet straw (FMS)	229:268	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	0	37	theme	millet	61:66	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	5	38	theme	solid	763:767	arg1	yield					777:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	5	38	theme	solid	763:767	arg1	biomass					800:806	biomass	800:806	biomass	800:806	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	6	39	theme	two-step	813:820	arg1	optimization					822:833	The two-step optimization	809:833	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design	809:1031	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	40	with	FMS	895:897	arg1	exo-1,4-β-xylosidase					955:974	ii) exo-1,4-β-xylosidase	951:974	ii) exo-1,4-β-xylosidase (BoGH43A)	951:984	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	40	with	FMS	895:897	arg1	CtXyn11A					928:935	CtXyn11A	928:935	CtXyn11A	928:935	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	40	with	FMS	895:897	arg1	endo-1,4-β-xylanase					907:925	endo-1,4-β-xylanase	907:925	i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C	904:945	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	6	40	with	FMS	895:897	arg1	BoGH43A					977:983	BoGH43A	977:983	BoGH43A	977:983	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31173827	0	41	theme	Finger	54:59	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	4	42	theme	holocellulose	681:693	arg1	content					695:701	the holocellulose content	677:701	the holocellulose content from 69.3% of raw powdered FMS	677:732	The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%.					
31173827	2	43	theme	FMS	345:347	arg1	composition					330:340	the carbohydrate composition	313:340	the carbohydrate composition of FMS	313:347	The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments.					
31173827	1	44	theme	millet	251:256	arg1	FMS					265:267	FMS	265:267	FMS	265:267	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	1	44	theme	millet	251:256	arg1	straw					258:262	the pretreated Finger millet straw	229:262	the pretreated Finger millet straw (FMS)	229:268	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	3	45	theme	biomass	628:634	arg1	yield					594:598	a total reducing sugar yield	571:598	a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass	571:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	45	theme	biomass	628:634	arg1	TRS					601:603	TRS	601:603	TRS	601:603	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	46	theme	%	496:496	arg1	NaOH					504:507	1% (w/v) NaOH	495:507	1% (w/v) NaOH combined with oven heating at 120 °C for 20 min	495:555	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	2	47	from	variation	300:308	arg1	composition					330:340	the carbohydrate composition	313:340	the carbohydrate composition of FMS	313:347	The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments.					
31173827	1	48	theme	straw	258:262	arg1	part					221:224	hemicellulose part	207:224	hemicellulose part of the pretreated Finger millet straw (FMS)	207:268	This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose.					
31173827	0	49	theme	Eleusine	69:76	arg1	straw					88:92	pretreated Finger millet (Eleusine coracana) straw	43:92	pretreated Finger millet (Eleusine coracana) straw	43:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	0	50	from	straw	88:92	arg1	hemicellulose					24:36	hemicellulose	24:36	hemicellulose from pretreated Finger millet (Eleusine coracana) straw	24:92	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	0	50	from	straw	88:92	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.	0:153	Enzymatic hydrolysis of hemicellulose from pretreated Finger millet (Eleusine coracana) straw by recombinant endo-1,4-β-xylanase and exo-1,4-β-xylosidase.					
31173827	3	51	theme	sugar	588:592	arg1	yield					594:598	a total reducing sugar yield	571:598	a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass	571:634	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	3	51	theme	sugar	588:592	arg1	TRS					601:603	TRS	601:603	TRS	601:603	The recombinant endo-1,4-β-xylanase (CtXyn11A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 °C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass.					
31173827	2	52	theme	different	380:388	arg1	pretreatments					390:402	different pretreatments	380:402	different pretreatments	380:402	The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments.					
31173827	5	53	theme	post-treatment	748:761	arg1	yield					777:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield	744:781	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	5	53	theme	post-treatment	748:761	arg1	biomass					800:806	biomass	800:806	biomass	800:806	The post-treatment solid biomass yield was 0.36 g/g raw biomass.					
31173827	6	54	from	optimization	822:833	arg1	FMS					895:897	the above pretreated FMS	874:897	the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5	874:1009	The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo-1,4-β-xylanase (CtXyn11A) at 55 °C and ii) exo-1,4-β-xylosidase (BoGH43A) at 37 °C, both at pH 7.5 by Box-Behnken design yielded the TRS of 70 mg/g pretreated biomass.					
31163700	3	0	theme	striata	321:327	arg1	BSP					345:347	BSP	345:347	BSP	345:347	In this study, Bletilla striata polysaccharide (BSP) was blended with PLA by a solvent method.					
31163700	3	0	theme	striata	321:327	arg1	polysaccharide					329:342	Bletilla striata polysaccharide	312:342	Bletilla striata polysaccharide (BSP)	312:348	In this study, Bletilla striata polysaccharide (BSP) was blended with PLA by a solvent method.					
31163700	5	1	theme	TG	613:614	arg1	data					616:619	TG data	613:619	TG data	613:619	TG data indicates that the composite film material has good thermal stability.					
31163700	7	2	theme	white	843:847	arg1	polysaccharide					855:868	white peony polysaccharide	843:868	white peony polysaccharide	843:868	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	6	3	theme	pure	790:793	arg1	film					799:802	the pure PLA film	786:802	the pure PLA film	786:802	Tensile tests show that the composite film is improved in rigidity and elasticity compared to the pure PLA film.					
31163700	1	4	theme	high	158:161	arg1	price					163:167	its high price	154:167	its high price	154:167	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	4	5	theme	°C	552:553	arg1	value					540:544	an extreme value	529:544	an extreme value of 68 °C	529:553	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	7	6	theme	PLA	834:836	arg1	modification					818:829	The blending modification	805:829	The blending modification of PLA with white peony polysaccharide	805:868	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	7	7	theme	PLA	963:965	arg1	properties					949:958	the thermal and mechanical properties	922:958	the thermal and mechanical properties of PLA	922:965	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	7	8	theme	PLA	899:901	arg1	cost					891:894	the cost	887:894	the cost of PLA	887:901	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	7	9	theme	blending	809:816	arg1	modification					818:829	The blending modification	805:829	The blending modification of PLA with white peony polysaccharide	805:868	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	4	10	theme	glass-transition	443:458	arg1	temperature					460:470	a higher glass-transition temperature	434:470	a higher glass-transition temperature	434:470	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	11	theme	DMA	392:394	arg1	data					396:399	DMA data	392:399	DMA data	392:399	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	1	12	theme	Polylactic	95:104	arg1	PLA					112:114	PLA	112:114	PLA	112:114	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	1	12	theme	Polylactic	95:104	arg1	acid					106:109	Polylactic acid	95:109	Polylactic acid (PLA)	95:115	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	2	13	used	used	260:263	arg2	methods					238:244	Modification methods	225:244	Modification methods	225:244	Modification methods are currently used to overcome these shortcomings.					
31163700	5	14	theme	composite	640:648	arg1	material					655:662	the composite film material	636:662	the composite film material	636:662	TG data indicates that the composite film material has good thermal stability.					
31163700	1	15	theme	high	170:173	arg1	brittleness					175:185	high brittleness	170:185	high brittleness	170:185	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	3	16	theme	solvent	376:382	arg1	method					384:389	a solvent method	374:389	a solvent method	374:389	In this study, Bletilla striata polysaccharide (BSP) was blended with PLA by a solvent method.					
31163700	4	17	theme	extreme	532:538	arg1	value					540:544	an extreme value	529:544	an extreme value of 68 °C	529:553	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	5	18	theme	good	668:671	arg1	stability					681:689	good thermal stability	668:689	good thermal stability	668:689	TG data indicates that the composite film material has good thermal stability.					
31163700	1	19	theme	low	191:193	arg1	temperature					212:222	low glass-transition temperature	191:222	low glass-transition temperature	191:222	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	5	20	contain	has	664:666	arg2	stability					681:689	good thermal stability	668:689	good thermal stability	668:689	TG data indicates that the composite film material has good thermal stability.					
31163700	5	20	contain	has	664:666	arg1	material					655:662	the composite film material	636:662	the composite film material	636:662	TG data indicates that the composite film material has good thermal stability.					
31163700	5	21	theme	thermal	673:679	arg1	stability					681:689	good thermal stability	668:689	good thermal stability	668:689	TG data indicates that the composite film material has good thermal stability.					
31163700	1	22	theme	glass-transition	195:210	arg1	temperature					212:222	low glass-transition temperature	191:222	low glass-transition temperature	191:222	Polylactic acid (PLA) is limited in its application due to its high price, high brittleness and low glass-transition temperature.					
31163700	0	23	theme	Bletilla	36:43	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Bletilla striata Polysaccharide/Polylactic Acid Composite.					
31163700	0	23	theme	Bletilla	36:43	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Bletilla striata Polysaccharide/Polylactic Acid Composite.					
31163700	4	24	theme	film	517:520	arg1	temperature					498:508	the glass-transition temperature	477:508	the glass-transition temperature of the film	477:520	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	25	theme	higher	436:441	arg1	temperature					460:470	a higher glass-transition temperature	434:470	a higher glass-transition temperature	434:470	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	26	contain	had	430:432	arg2	temperature					460:470	a higher glass-transition temperature	434:470	a higher glass-transition temperature	434:470	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	26	contain	had	430:432	arg1	film					425:428	the BSP/PLA film	413:428	the BSP/PLA film	413:428	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	0	27	theme	Acid	79:82	arg1	Composite					84:92	Polysaccharide/Polylactic Acid Composite	53:92	Polysaccharide/Polylactic Acid Composite	53:92	Preparation and Characterization of Bletilla striata Polysaccharide/Polylactic Acid Composite.					
31163700	2	28	theme	Modification	225:236	arg1	methods					238:244	Modification methods	225:244	Modification methods	225:244	Modification methods are currently used to overcome these shortcomings.					
31163700	6	29	theme	composite	720:728	arg1	film					730:733	the composite film	716:733	the composite film	716:733	Tensile tests show that the composite film is improved in rigidity and elasticity compared to the pure PLA film.					
31163700	0	30	theme	Polysaccharide/Polylactic	53:77	arg1	Composite					84:92	Polysaccharide/Polylactic Acid Composite	53:92	Polysaccharide/Polylactic Acid Composite	53:92	Preparation and Characterization of Bletilla striata Polysaccharide/Polylactic Acid Composite.					
31163700	3	31	theme	Bletilla	312:319	arg1	BSP					345:347	BSP	345:347	BSP	345:347	In this study, Bletilla striata polysaccharide (BSP) was blended with PLA by a solvent method.					
31163700	3	31	theme	Bletilla	312:319	arg1	polysaccharide					329:342	Bletilla striata polysaccharide	312:342	Bletilla striata polysaccharide (BSP)	312:348	In this study, Bletilla striata polysaccharide (BSP) was blended with PLA by a solvent method.					
31163700	7	32	theme	mechanical	938:947	arg1	properties					949:958	the thermal and mechanical properties	922:958	the thermal and mechanical properties of PLA	922:965	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	5	33	theme	film	650:653	arg1	material					655:662	the composite film material	636:662	the composite film material	636:662	TG data indicates that the composite film material has good thermal stability.					
31163700	6	34	theme	PLA	795:797	arg1	film					799:802	the pure PLA film	786:802	the pure PLA film	786:802	Tensile tests show that the composite film is improved in rigidity and elasticity compared to the pure PLA film.					
31163700	4	35	theme	BSP/PLA	417:423	arg1	film					425:428	the BSP/PLA film	413:428	the BSP/PLA film	413:428	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	7	36	theme	thermal	926:932	arg1	properties					949:958	the thermal and mechanical properties	922:958	the thermal and mechanical properties of PLA	922:965	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	7	37	theme	peony	849:853	arg1	polysaccharide					855:868	white peony polysaccharide	843:868	white peony polysaccharide	843:868	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	7	38	with	modification	818:829	arg1	polysaccharide					855:868	white peony polysaccharide	843:868	white peony polysaccharide	843:868	The blending modification of PLA with white peony polysaccharide not only reduces the cost of PLA, but also improves the thermal and mechanical properties of PLA.					
31163700	4	39	theme	polysaccharide	588:601	arg1	%					610:610	0.8%	607:610	0.8%	607:610	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	39	theme	polysaccharide	588:601	arg1	proportion					564:573	the proportion	560:573	the proportion of the chalk polysaccharide	560:601	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	4	40	theme	glass-transition	481:496	arg1	temperature					498:508	the glass-transition temperature	477:508	the glass-transition temperature of the film	477:520	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31163700	6	41	theme	Tensile	692:698	arg1	tests					700:704	Tensile tests	692:704	Tensile tests	692:704	Tensile tests show that the composite film is improved in rigidity and elasticity compared to the pure PLA film.					
31163700	4	42	theme	chalk	582:586	arg1	polysaccharide					588:601	the chalk polysaccharide	578:601	the chalk polysaccharide	578:601	DMA data showed that the BSP/PLA film had a higher glass-transition temperature, and the glass-transition temperature of the film showed an extreme value of 68 °C when the proportion of the chalk polysaccharide was 0.8%.					
31590875	0	0	theme	multi-metal	93:103	arg1	KMS3-1					131:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	7	1	theme	multi-metal	1091:1101	arg1	KMS3-1					1129:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	7	2	theme	metal	1209:1213	arg1	removal					1215:1221	metal removal	1209:1221	metal removal	1209:1221	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	7	2	theme	metal	1209:1213	arg1	biotechnological					1153:1168	biotechnological	1153:1168	biotechnological	1153:1168	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	7	3	theme	EPS-producing	1077:1089	arg1	KMS3-1					1129:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	7	4	theme	resistant	1103:1111	arg1	KMS3-1					1129:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	3	5	theme	optimum	515:521	arg1	conditions					523:532	optimum conditions	515:532	optimum conditions for exopolysaccharides (EPS) production	515:572	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	1	6	theme	bacteria	230:237	arg1	isolation					143:151	isolation	143:151	isolation	143:151	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	6	theme	bacteria	230:237	arg1	screening					154:162	screening	154:162	screening	154:162	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	6	theme	bacteria	230:237	arg1	identification					169:182	identification	169:182	identification	169:182	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	0	7	theme	Bacillus	115:122	arg1	KMS3-1					131:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	7	8	theme	potential	1067:1075	arg1	KMS3-1					1129:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	1	9	dep	isolation	143:151	arg1	The					139:141	The	139:141	The	139:141	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	4	10	theme	sucrose	745:751	arg1	concentrations					727:740	the optimum concentrations	715:740	the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L)	715:805	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	10	theme	sucrose	745:751	arg1	5 g/L					812:816	5 g/L	812:816	5 g/L	812:816	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	0	11	theme	resistant	105:113	arg1	KMS3-1					131:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	multi-metal resistant Bacillus cereus KMS3-1	93:136	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	4	12	theme	yeast	757:761	arg1	extract					763:769	yeast extract	757:769	yeast extract	757:769	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	2	13	dep	S2-2	322:325	arg1	resistance					359:368	higher multi-metal resistance	340:368	higher multi-metal resistance	340:368	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	1	14	theme	polluted	244:251	arg1	samples					270:276	polluted coastal sediment samples	244:276	polluted coastal sediment samples	244:276	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	15	from	samples	270:276	arg1	isolation					143:151	isolation	143:151	isolation	143:151	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	15	from	samples	270:276	arg1	screening					154:162	screening	154:162	screening	154:162	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	15	from	samples	270:276	arg1	identification					169:182	identification	169:182	identification	169:182	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	0	16	dep	Bacillus	115:122	arg1	cereus					124:129	cereus	124:129	cereus	124:129	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	7	17	theme	industrial	1174:1183	arg1	application					1185:1195	industrial application	1174:1195	industrial application	1174:1195	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	7	18	dep	suggested	1053:1061	arg1	used					1145:1148	used	1145:1148	suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal	1053:1221	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	1	19	theme	multi-metal	187:197	arg1	bacteria					230:237	multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria	187:237	multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria	187:237	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	5	20	theme	HPLC	909:912	arg1	analysis					914:921	HPLC analysis	909:921	HPLC analysis	909:921	Heteropolysaccharide nature of EPS determined by FTIR, TLC, and HPLC analysis, consist of mannose, rhamnose, glucose, and xylose.					
31590875	1	21	theme	resistant	199:207	arg1	bacteria					230:237	multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria	187:237	multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria	187:237	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	2	22	theme	Bacillus	430:437	arg1	KMS3-1					446:451	Bacillus cereus KMS3-1	430:451	Bacillus cereus KMS3-1	430:451	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	1	23	theme	coastal	253:259	arg1	samples					270:276	polluted coastal sediment samples	244:276	polluted coastal sediment samples	244:276	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	2	24	dep	Bacillus	430:437	arg1	cereus					439:444	cereus	439:444	cereus	439:444	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	7	25	dep	Bacillus	1113:1120	arg1	cereus					1122:1127	cereus	1122:1127	cereus	1122:1127	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	4	26	theme	extract	763:769	arg1	concentrations					727:740	the optimum concentrations	715:740	the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L)	715:805	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	26	theme	extract	763:769	arg1	5 g/L					812:816	5 g/L	812:816	5 g/L	812:816	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	2	27	theme	multi-metal	347:357	arg1	resistance					359:368	higher multi-metal resistance	340:368	higher multi-metal resistance	340:368	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	1	28	theme	sediment	261:268	arg1	samples					270:276	polluted coastal sediment samples	244:276	polluted coastal sediment samples	244:276	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	3	29	theme	incubation	587:596	arg1	time					598:601	incubation time 120 h	587:607	incubation time 120 h	587:607	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	2	30	dep	isolates	313:320	arg1	isolates					313:320	the isolates S2-2 and S3-2 had higher multi-metal resistance	309:368	the isolates S2-2 and S3-2 had higher multi-metal resistance	309:368	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	2	30	dep	isolates	313:320	arg1	S3-2 had					331:338	S3-2 had	331:338	S3-2 had	331:338	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	2	30	dep	isolates	313:320	arg1	S2-2					322:325	S2-2	322:325	S2-2	322:325	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	6	31	theme	flocculation	1022:1033	arg1	activity					1035:1042	strong emulsifying and flocculation activity	999:1042	strong emulsifying and flocculation activity	999:1042	In addition, EPS showed strong emulsifying and flocculation activity.					
31590875	6	32	theme	strong	999:1004	arg1	activity					1035:1042	strong emulsifying and flocculation activity	999:1042	strong emulsifying and flocculation activity	999:1042	In addition, EPS showed strong emulsifying and flocculation activity.					
31590875	0	33	theme	compositional	14:26	arg1	analysis					28:35	compositional analysis	14:35	compositional analysis	14:35	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	3	34	theme	exopolysaccharides	538:555	arg1	production					563:572	exopolysaccharides (EPS) production	538:572	exopolysaccharides (EPS) production	538:572	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	3	35	theme	yeast	636:640	arg1	extract					642:648	10 g/L yeast extract	629:648	10 g/L yeast extract	629:648	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	4	36	theme	composite	684:692	arg1	design					694:699	central composite design	676:699	central composite design	676:699	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	2	37	theme	Pseudomonas	393:403	arg1	KMS2-2					419:424	Pseudomonas pachastrellae KMS2-2	393:424	Pseudomonas pachastrellae KMS2-2	393:424	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	1	38	dep	resistant	199:207	arg1	Pb					218:219	Pb	218:219	Pb	218:219	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	38	dep	resistant	199:207	arg1	Zn					226:227	Zn	226:227	Zn	226:227	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	38	dep	resistant	199:207	arg1	Cu					214:215	Cu	214:215	Cu	214:215	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	1	38	dep	resistant	199:207	arg1	Cd					210:211	Cd	210:211	Cd	210:211	The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed.					
31590875	6	39	theme	emulsifying	1006:1016	arg1	activity					1035:1042	strong emulsifying and flocculation activity	999:1042	strong emulsifying and flocculation activity	999:1042	In addition, EPS showed strong emulsifying and flocculation activity.					
31590875	4	40	theme	central	676:682	arg1	design					694:699	central composite design	676:699	central composite design	676:699	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	7	41	theme	Bacillus	1113:1120	arg1	KMS3-1					1129:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1	1063:1134	Results suggested the potential EPS-producing multi-metal resistant Bacillus cereus KMS3-1 could be used in biotechnological and industrial application, especially metal removal.					
31590875	5	42	theme	EPS	876:878	arg1	nature					866:871	Heteropolysaccharide nature	845:871	Heteropolysaccharide nature	845:871	Heteropolysaccharide nature of EPS determined by FTIR, TLC, and HPLC analysis, consist of mannose, rhamnose, glucose, and xylose.					
31590875	3	43	theme	10 g/L	629:634	arg1	extract					642:648	10 g/L yeast extract	629:648	10 g/L yeast extract	629:648	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	2	44	dep	Pseudomonas	393:403	arg1	pachastrellae					405:417	pachastrellae	405:417	pachastrellae	405:417	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	3	45	theme	One-variable-at-a	468:484	arg1	approach					491:498	One-variable-at-a time approach	468:498	One-variable-at-a time approach	468:498	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	4	46	theme	higher	775:780	arg1	8.9 g/L					798:804	8.9 g/L	798:804	8.9 g/L	798:804	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	46	theme	higher	775:780	arg1	production					786:795	higher EPS production	775:795	higher EPS production (8.9 g/L)	775:805	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	47	theme	EPS	782:784	arg1	8.9 g/L					798:804	8.9 g/L	798:804	8.9 g/L	798:804	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	47	theme	EPS	782:784	arg1	production					786:795	higher EPS production	775:795	higher EPS production (8.9 g/L)	775:805	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	3	48	theme	time	486:489	arg1	approach					491:498	One-variable-at-a time approach	468:498	One-variable-at-a time approach	468:498	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
31590875	4	49	theme	optimum	719:725	arg1	concentrations					727:740	the optimum concentrations	715:740	the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L)	715:805	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	4	49	theme	optimum	719:725	arg1	5 g/L					812:816	5 g/L	812:816	5 g/L	812:816	Further, optimization by central composite design revealed that the optimum concentrations of sucrose and yeast extract for higher EPS production (8.9 g/L) were 5 g/L, and 30 g/L, respectively.					
31590875	2	50	theme	higher	340:345	arg1	resistance					359:368	higher multi-metal resistance	340:368	higher multi-metal resistance	340:368	In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively.					
31590875	5	51	theme	Heteropolysaccharide	845:864	arg1	nature					866:871	Heteropolysaccharide nature	845:871	Heteropolysaccharide nature	845:871	Heteropolysaccharide nature of EPS determined by FTIR, TLC, and HPLC analysis, consist of mannose, rhamnose, glucose, and xylose.					
31590875	0	52	theme	exopolysaccharides	62:79	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	0	52	theme	exopolysaccharides	62:79	arg1	analysis					28:35	compositional analysis	14:35	compositional analysis	14:35	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	0	52	theme	exopolysaccharides	62:79	arg1	characterization					42:57	characterization	42:57	characterization	42:57	Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.					
31590875	3	53	theme	5 g/L	610:614	arg1	sucrose					616:622	5 g/L sucrose	610:622	5 g/L sucrose	610:622	One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.0, incubation time 120 h, 5 g/L sucrose, and 10 g/L yeast extract.					
29578012	7	0	theme	-α-Araf-	1019:1026	arg1	1→					1041:1042	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	1→	1041:1042	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	1	theme	TNF-α	1251:1255	arg1	secretions					1237:1246	the secretions	1233:1246	the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1233:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	2	2	from	herb	187:190	arg1	China					205:209	Southwest China	195:209	Southwest China	195:209	has been widely used as a medicinal and edible herb in Southwest China and Southeast Asia.					
29578012	2	2	from	herb	187:190	arg1	Asia					225:228	Asia	225:228	Asia	225:228	has been widely used as a medicinal and edible herb in Southwest China and Southeast Asia.					
29578012	7	3	theme	1→5	1015:1017	arg1	1→					1041:1042	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	1→	1041:1042	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	4	theme	IL-6	1258:1261	arg1	secretions					1237:1246	the secretions	1233:1246	the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1233:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	10	5	from	macrophages	1420:1430	arg1	secretions					1372:1381	the secretions	1368:1381	the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1368:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	7	6	theme	-α-Rhap-	1006:1013	arg1	1→					1041:1042	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	1→	1041:1042	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	8	7	theme	immunomodulatory	1084:1099	arg1	assays					1101:1106	The immunomodulatory assays	1080:1106	The immunomodulatory assays	1080:1106	The immunomodulatory assays revealed the dual-functionalities of NFP-1.					
29578012	11	8	theme	due	1606:1608	arg1	food					1601:1604	an ideal medicinal or functional food	1568:1604	an ideal medicinal or functional food due to its dual immunomodulatory activities	1568:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	8	theme	due	1606:1608	arg1	fordii					1552:1557	Nervilia fordii	1543:1557	Nervilia fordii	1543:1557	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	7	9	theme	→2,4	1001:1004	arg1	chains					991:996	two branch chains	980:996	two branch chains of →2,4	980:1004	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	6	10	theme	galacturonic	779:790	arg1	acid					792:795	galacturonic acid	779:795	galacturonic acid	779:795	NFP-1 mainly consists of galactose, arabinose, rhamnose, and galacturonic acid.					
29578012	7	11	theme	GC-MS	830:834	arg1	analysis					836:843	methylation and GC-MS analysis	814:843	analysis	836:843	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	11	12	theme	functional	1590:1599	arg1	food					1601:1604	an ideal medicinal or functional food	1568:1604	an ideal medicinal or functional food due to its dual immunomodulatory activities	1568:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	12	theme	functional	1590:1599	arg1	fordii					1552:1557	Nervilia fordii	1543:1557	Nervilia fordii	1543:1557	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	7	13	theme	methylation	814:824	arg1	analysis					836:843	methylation and GC-MS analysis	814:843	analysis	836:843	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	0	14	from	Nervilia	91:98	arg1	polysaccharide					71:84	a novel polysaccharide	63:84	a novel polysaccharide from Nervilia fordii	63:105	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	0	14	from	Nervilia	91:98	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	0	14	from	Nervilia	91:98	arg1	activities					49:58	immunomodulating activities	32:58	immunomodulating activities	32:58	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	11	15	theme	immunomodulatory	1622:1637	arg1	activities					1639:1648	its dual immunomodulatory activities	1613:1648	its dual immunomodulatory activities	1613:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	16	theme	dual	1617:1620	arg1	activities					1639:1648	its dual immunomodulatory activities	1613:1648	its dual immunomodulatory activities	1613:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	3	17	theme	polysaccharidewith	273:290	arg1	purity					294:299	a new water-soluble polysaccharidewith a purity	253:299	a new water-soluble polysaccharidewith a purity of 97.8%	253:308	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	3	17	theme	polysaccharidewith	273:290	arg1	NFP-1					246:250	NFP-1	246:250	NFP-1	246:250	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	8	18	theme	NFP-1	1145:1149	arg1	dual-functionalities					1121:1140	the dual-functionalities	1117:1140	the dual-functionalities of NFP-1	1117:1149	The immunomodulatory assays revealed the dual-functionalities of NFP-1.					
29578012	3	19	theme	new	255:257	arg1	purity					294:299	a new water-soluble polysaccharidewith a purity	253:299	a new water-soluble polysaccharidewith a purity of 97.8%	253:308	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	3	19	theme	new	255:257	arg1	NFP-1					246:250	NFP-1	246:250	NFP-1	246:250	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	7	20	theme	4→1	955:957	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	20	theme	4→1	955:957	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	21	theme	1→2	942:944	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	21	theme	1→2	942:944	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	11	22	theme	Nervilia	1543:1550	arg1	fordii					1552:1557	Nervilia fordii	1543:1557	Nervilia fordii	1543:1557	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	22	theme	Nervilia	1543:1550	arg1	food					1601:1604	an ideal medicinal or functional food	1568:1604	an ideal medicinal or functional food due to its dual immunomodulatory activities	1568:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	0	23	theme	polysaccharide	71:84	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	0	23	theme	polysaccharide	71:84	arg1	activities					49:58	immunomodulating activities	32:58	immunomodulating activities	32:58	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	11	24	theme	medicinal	1577:1585	arg1	food					1601:1604	an ideal medicinal or functional food	1568:1604	an ideal medicinal or functional food due to its dual immunomodulatory activities	1568:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	24	theme	medicinal	1577:1585	arg1	fordii					1552:1557	Nervilia fordii	1543:1557	Nervilia fordii	1543:1557	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	5	25	theme	high	583:586	arg1	HPLC					623:626	HPLC	623:626	HPLC	623:626	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	5	25	theme	high	583:586	arg1	chromatography					607:620	high performance liquid chromatography	583:620	high performance liquid chromatography (HPLC)	583:627	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	4	26	theme	high	478:481	arg1	HPGPC					526:530	HPGPC	526:530	HPGPC	526:530	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	4	26	theme	high	478:481	arg1	chromatography					510:523	high performance gel-permeation chromatography	478:523	high performance gel-permeation chromatography (HPGPC)	478:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	1	27	dep	Schltr	132:137	arg1	Nervilia					108:115	Nervilia	108:115	Nervilia	108:115	Nervilia fordii (Hance) Schltr.					
29578012	1	27	dep	Schltr	132:137	arg1	Hance					125:129	Hance	125:129	Hance	125:129	Nervilia fordii (Hance) Schltr.					
29578012	5	28	theme	liquid	600:605	arg1	HPLC					623:626	HPLC	623:626	HPLC	623:626	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	5	28	theme	liquid	600:605	arg1	chromatography					607:620	high performance liquid chromatography	583:620	high performance liquid chromatography (HPLC)	583:627	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	4	29	theme	molecular	436:444	arg1	weight					446:451	a relative molecular weight	425:451	a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC)	425:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	7	30	theme	-α-Rhap-	905:912	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	30	theme	-α-Rhap-	905:912	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	31	theme	2→	914:915	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	31	theme	2→	914:915	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	0	32	from	activities	49:58	arg1	Nervilia					91:98	Nervilia	91:98	Nervilia	91:98	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	3	33	theme	Sephadex	384:391	arg1	chromatography					399:412	DEAE-cellulose and Sephadex G-100 chromatography	365:412	chromatography	399:412	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	0	34	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	7	35	theme	→4	902:903	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	35	theme	→4	902:903	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	3	36	theme	water	329:333	arg1	extract					335:341	water extract	329:341	water extract of Nervilia fordii	329:360	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	7	37	contain	containing	969:978	arg2	chains					991:996	two branch chains	980:996	two branch chains of →2,4	980:1004	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	37	contain	containing	969:978	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	37	contain	containing	969:978	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	38	theme	1→	1041:1042	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	38	theme	1→	1041:1042	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	39	theme	nitric	1202:1207	arg1	NO					1216:1217	NO	1216:1217	NO	1216:1217	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	9	39	theme	nitric	1202:1207	arg1	oxide					1209:1213	nitric oxide	1202:1213	nitric oxide (NO)	1202:1218	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	9	40	from	IL-6	1258:1261	arg1	macrophages					1286:1296	RAW264.7 macrophages	1277:1296	RAW264.7 macrophages	1277:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	3	41	theme	DEAE-cellulose	365:378	arg1	chromatography					399:412	DEAE-cellulose and Sephadex G-100 chromatography	365:412	chromatography	399:412	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	2	42	theme	medicinal	166:174	arg1	herb					187:190	a medicinal and edible herb	164:190	herb	187:190	has been widely used as a medicinal and edible herb in Southwest China and Southeast Asia.					
29578012	7	43	theme	-α-Araf-	1032:1039	arg1	1→					1041:1042	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	1→	1041:1042	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	44	from	TNF-α	1251:1255	arg1	macrophages					1286:1296	RAW264.7 macrophages	1277:1296	RAW264.7 macrophages	1277:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	10	45	from	secretions	1372:1381	arg1	macrophages					1420:1430	RAW264.7 macrophages	1411:1430	RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1411:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	3	46	theme	fordii	355:360	arg1	extract					335:341	water extract	329:341	water extract of Nervilia fordii	329:360	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	4	47	theme	gel-permeation	495:508	arg1	HPGPC					526:530	HPGPC	526:530	HPGPC	526:530	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	4	47	theme	gel-permeation	495:508	arg1	chromatography					510:523	high performance gel-permeation chromatography	478:523	high performance gel-permeation chromatography (HPGPC)	478:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	10	48	theme	NO	1356:1357	arg1	production					1342:1351	the production	1338:1351	the production of NO	1338:1357	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	7	49	theme	1→3	1028:1030	arg1	1→					1041:1042	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	1→	1041:1042	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	50	from	secretions	1237:1246	arg1	macrophages					1286:1296	RAW264.7 macrophages	1277:1296	RAW264.7 macrophages	1277:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	10	51	from	TNF-α	1386:1390	arg1	macrophages					1420:1430	RAW264.7 macrophages	1411:1430	RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1411:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	10	52	theme	IL-10	1501:1505	arg1	meanwhile					1507:1515	IL-10 meanwhile	1501:1515	IL-10 meanwhile	1501:1515	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	10	53	from	IL-6	1393:1396	arg1	macrophages					1420:1430	RAW264.7 macrophages	1411:1430	RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1411:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	9	54	from	IL-1β	1268:1272	arg1	macrophages					1286:1296	RAW264.7 macrophages	1277:1296	RAW264.7 macrophages	1277:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	2	55	theme	edible	180:185	arg1	herb					187:190	a medicinal and edible herb	164:190	herb	187:190	has been widely used as a medicinal and edible herb in Southwest China and Southeast Asia.					
29578012	10	56	from	IL-1β	1402:1406	arg1	macrophages					1420:1430	RAW264.7 macrophages	1411:1430	RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1411:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	5	57	with	hydrolysate	663:673	arg1	PMP					712:714	PMP	712:714	PMP	712:714	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	5	57	with	hydrolysate	663:673	arg1	1-phenyl-3-methyl-5-pyrazolone					680:709	1-phenyl-3-methyl-5-pyrazolone	680:709	1-phenyl-3-methyl-5-pyrazolone (PMP)	680:715	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	10	58	theme	IL-6	1393:1396	arg1	secretions					1372:1381	the secretions	1368:1381	the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1368:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	7	59	theme	structure	859:867	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	59	theme	structure	859:867	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	10	60	theme	TNF-α	1386:1390	arg1	secretions					1372:1381	the secretions	1368:1381	the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1368:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	10	61	theme	RAW264.7	1411:1418	arg1	macrophages					1420:1430	RAW264.7 macrophages	1411:1430	RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1411:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	9	62	theme	IL-1β	1268:1272	arg1	secretions					1237:1246	the secretions	1233:1246	the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1233:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	4	63	theme	performance	483:493	arg1	HPGPC					526:530	HPGPC	526:530	HPGPC	526:530	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	4	63	theme	performance	483:493	arg1	chromatography					510:523	high performance gel-permeation chromatography	478:523	high performance gel-permeation chromatography (HPGPC)	478:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	10	64	theme	IL-1β	1402:1406	arg1	secretions					1372:1381	the secretions	1368:1381	the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS)	1368:1468	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	7	65	theme	branch	984:989	arg1	chains					991:996	two branch chains	980:996	two branch chains of →2,4	980:1004	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	9	66	theme	RAW264.7	1277:1284	arg1	macrophages					1286:1296	RAW264.7 macrophages	1277:1296	RAW264.7 macrophages	1277:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	4	67	theme	relative	427:434	arg1	weight					446:451	a relative molecular weight	425:451	a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC)	425:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	7	68	theme	-α-Rhap-	946:953	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	68	theme	-α-Rhap-	946:953	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	3	69	theme	a	292:292	arg1	purity					294:299	a new water-soluble polysaccharidewith a purity	253:299	a new water-soluble polysaccharidewith a purity of 97.8%	253:308	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	3	69	theme	a	292:292	arg1	NFP-1					246:250	NFP-1	246:250	NFP-1	246:250	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	5	70	theme	monosaccharide	538:551	arg1	compositions					553:564	Its monosaccharide compositions	534:564	Its monosaccharide compositions	534:564	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	5	71	theme	performance	588:598	arg1	HPLC					623:626	HPLC	623:626	HPLC	623:626	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	5	71	theme	performance	588:598	arg1	chromatography					607:620	high performance liquid chromatography	583:620	high performance liquid chromatography (HPLC)	583:627	Its monosaccharide compositions were analyzed by high performance liquid chromatography (HPLC) after pre-column derivatizing its hydrolysate with 1-phenyl-3-methyl-5-pyrazolone (PMP).					
29578012	3	72	theme	water-soluble	259:271	arg1	purity					294:299	a new water-soluble polysaccharidewith a purity	253:299	a new water-soluble polysaccharidewith a purity of 97.8%	253:308	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	3	72	theme	water-soluble	259:271	arg1	NFP-1					246:250	NFP-1	246:250	NFP-1	246:250	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	7	73	theme	-β-Galp-	1067:1074	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	73	theme	-β-Galp-	1067:1074	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	74	theme	-α-Rhap-	933:940	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	74	theme	-α-Rhap-	933:940	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	3	75	theme	%	308:308	arg1	purity					294:299	a new water-soluble polysaccharidewith a purity	253:299	a new water-soluble polysaccharidewith a purity of 97.8%	253:308	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	3	75	theme	%	308:308	arg1	NFP-1					246:250	NFP-1	246:250	NFP-1	246:250	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	7	76	theme	1→4	1063:1065	arg1	-β-Galp-					1067:1074	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	-β-Galp-	1067:1074	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	77	theme	1→2	929:931	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	77	theme	1→2	929:931	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	0	78	from	characterization	11:26	arg1	Nervilia					91:98	Nervilia	91:98	Nervilia	91:98	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	7	79	theme	-α-Rhap-	1054:1061	arg1	-β-Galp-					1067:1074	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	-β-Galp-	1067:1074	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	80	theme	-α-GalpA-	919:927	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	80	theme	-α-GalpA-	919:927	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	11	81	theme	ideal	1571:1575	arg1	food					1601:1604	an ideal medicinal or functional food	1568:1604	an ideal medicinal or functional food due to its dual immunomodulatory activities	1568:1648	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	11	81	theme	ideal	1571:1575	arg1	fordii					1552:1557	Nervilia fordii	1543:1557	Nervilia fordii	1543:1557	Our study suggested that Nervilia fordii could be an ideal medicinal or functional food due to its dual immunomodulatory activities.					
29578012	7	82	theme	→2,4	1049:1052	arg1	-β-Galp-					1067:1074	-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-	1006:1074	-β-Galp-	1067:1074	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	4	83	theme	950 kDa	456:462	arg1	weight					446:451	a relative molecular weight	425:451	a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC)	425:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	3	84	theme	G-100	393:397	arg1	chromatography					399:412	DEAE-cellulose and Sephadex G-100 chromatography	365:412	chromatography	399:412	In this study, NFP-1, a new water-soluble polysaccharidewith a purity of 97.8%, was purified from water extract of Nervilia fordii by DEAE-cellulose and Sephadex G-100 chromatography.					
29578012	0	85	theme	immunomodulating	32:47	arg1	activities					49:58	immunomodulating activities	32:58	immunomodulating activities	32:58	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	9	86	theme	oxide	1209:1213	arg1	secretion					1189:1197	the secretion	1185:1197	the secretion of nitric oxide (NO)	1185:1218	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	4	87	contain	has	421:423	arg2	weight					446:451	a relative molecular weight	425:451	a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC)	425:531	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	4	87	contain	has	421:423	arg1	NFP-1					415:419	NFP-1	415:419	NFP-1	415:419	NFP-1 has a relative molecular weight of 950 kDa determined by high performance gel-permeation chromatography (HPGPC).					
29578012	0	88	theme	novel	65:69	arg1	polysaccharide					71:84	a novel polysaccharide	63:84	a novel polysaccharide from Nervilia fordii	63:105	Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.					
29578012	2	89	theme	Southwest	195:203	arg1	China					205:209	Southwest China	195:209	Southwest China	195:209	has been widely used as a medicinal and edible herb in Southwest China and Southeast Asia.					
29578012	10	90	theme	meanwhile	1507:1515	arg1	production					1487:1496	the production	1483:1496	the production of IL-10 meanwhile	1483:1515	NFP-1 could also significantly inhibit the production of NO, depress the secretions of TNF-α, IL-6 and IL-1β in RAW264.7 macrophages activated by lipopolysaccharide (LPS), and promote the production of IL-10 meanwhile.					
29578012	9	91	from	macrophages	1286:1296	arg1	secretions					1237:1246	the secretions	1233:1246	the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages	1233:1296	NFP-1 could significantly induce the secretion of nitric oxide (NO), and promote the secretions of TNF-α, IL-6, and IL-1β in RAW264.7 macrophages.					
29578012	7	92	theme	NFP-1	877:881	arg1	unit					869:872	the structure unit	855:872	the structure unit of NFP-1	855:881	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
29578012	7	92	theme	NFP-1	877:881	arg1	-β-Galp-T					959:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T	902:967	→4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→	902:1077	Based on FT-IR, methylation and GC-MS analysis, and NMR, the structure unit of NFP-1 was established as →4)-α-Rhap-(2→ 4)-α-GalpA-(1→2)-α-Rhap-(1→2)-α-Rhap-(4→1)-β-Galp-T containing two branch chains of →2,4)-α-Rhap-(1→5)-α-Araf-(1→3)-α-Araf-(1→, and →2,4)-α-Rhap-(1→4)-β-Galp-(1→.					
30684576	6	0	theme	fucoidan	955:962	arg1	composition					940:950	The monosaccharide composition	921:950	The monosaccharide composition of fucoidan	921:962	The monosaccharide composition of fucoidan included fucose (34.13%), mannose (30.70%), galactose (23.19%), xylose (9.35%) and glucose (2.65%).					
30684576	8	1	theme	reducing	1243:1250	arg1	power					1252:1256	reducing power	1243:1256	reducing power (0.182 Abs)	1243:1268	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	1	theme	reducing	1243:1250	arg1	Abs					1265:1267	0.182 Abs	1259:1267	0.182 Abs	1259:1267	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	9	2	theme	HeLa	1339:1342	arg1	cells					1344:1348	HeLa cells	1339:1348	HeLa cells	1339:1348	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	5	3	theme	molecular	795:803	arg1	weight					805:810	molecular weight	795:810	molecular weight	795:810	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	7	4	theme	average	1068:1074	arg1	weight					1086:1091	The average molecular weight	1064:1091	The average molecular weight of the extracted fucoidan	1064:1117	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	7	4	theme	average	1068:1074	arg1	694 kDa					1123:1129	694 kDa	1123:1129	694 kDa	1123:1129	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	1	5	theme	extraction	264:273	arg1	conditions					275:284	extraction conditions	264:284	extraction conditions	264:284	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	6	6	dep	included	964:971	arg1	%					986:986	34.13%	981:986	34.13%	981:986	The monosaccharide composition of fucoidan included fucose (34.13%), mannose (30.70%), galactose (23.19%), xylose (9.35%) and glucose (2.65%).					
30684576	10	7	theme	NO	1477:1478	arg1	production					1480:1489	maximum NO production	1469:1489	maximum NO production	1469:1489	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	6	8	theme	monosaccharide	925:938	arg1	composition					940:950	The monosaccharide composition	921:950	The monosaccharide composition of fucoidan	921:962	The monosaccharide composition of fucoidan included fucose (34.13%), mannose (30.70%), galactose (23.19%), xylose (9.35%) and glucose (2.65%).					
30684576	2	9	dep	found	380:384	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	9	dep	found	380:384	arg1	temperature					431:441	extraction temperature	420:441	extraction temperature of 150 °C	420:451	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	9	dep	found	380:384	arg1	time					404:407	extraction time	393:407	extraction time of 29 min	393:417	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	5	10	theme	extract	884:890	arg1	composition					782:792	Chemical and monosaccharide composition	754:792	composition	782:792	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	5	10	theme	extract	884:890	arg1	weight					805:810	molecular weight	795:810	molecular weight	795:810	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	5	10	theme	extract	884:890	arg1	activities					866:875	the antioxidant, anticancer and immunomodulatory activities	817:875	the antioxidant, anticancer and immunomodulatory activities of the extract	817:890	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	5	11	theme	Chemical	754:761	arg1	composition					782:792	Chemical and monosaccharide composition	754:792	composition	782:792	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	3	12	theme	fucoidan	502:509	arg1	%					557:557	25.98%	552:557	25.98%	552:557	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	3	12	theme	fucoidan	502:509	arg1	yield					511:515	The fucoidan yield	498:515	The fucoidan yield under these optimum conditions	498:546	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	5	13	theme	antioxidant	821:831	arg1	activities					866:875	the antioxidant, anticancer and immunomodulatory activities	817:875	the antioxidant, anticancer and immunomodulatory activities of the extract	817:890	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	2	14	theme	29 min	412:417	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	14	theme	29 min	412:417	arg1	temperature					431:441	extraction temperature	420:441	extraction temperature of 150 °C	420:451	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	14	theme	29 min	412:417	arg1	time					404:407	extraction time	393:407	extraction time of 29 min	393:417	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	1	15	attach	isolated	172:179	arg2	fucoidan					153:160	fucoidan	153:160	fucoidan	153:160	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	15	attach	isolated	172:179	arg1	Nizamuddinia					186:197	Nizamuddinia	186:197	Nizamuddinia	186:197	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	15	attach	isolated	172:179	arg2	polysaccharide					137:150	A sulfated polysaccharide	126:150	A sulfated polysaccharide (fucoidan)	126:161	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	2	16	theme	21 g/mL	489:495	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	16	theme	21 g/mL	489:495	arg1	temperature					431:441	extraction temperature	420:441	extraction temperature of 150 °C	420:451	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	16	theme	21 g/mL	489:495	arg1	time					404:407	extraction time	393:407	extraction time of 29 min	393:417	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	0	17	dep	Nizamuddinia	101:112	arg1	zanardinii					114:123	Nizamuddinia zanardinii	101:123	Nizamuddinia zanardinii	101:123	Subcritical water extraction as an efficient technique to isolate biologically-active fucoidans from Nizamuddinia zanardinii.					
30684576	10	18	theme	fucoidan	1532:1539	arg1	concentration					1541:1553	a 50 μg/mL fucoidan concentration	1521:1553	a 50 μg/mL fucoidan concentration	1521:1553	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	8	19	theme	appreciable	1194:1204	arg1	scavenging					1219:1228	appreciable ABTS radical scavenging	1194:1228	appreciable ABTS radical scavenging (70.35%)	1194:1237	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	19	theme	appreciable	1194:1204	arg1	%					1236:1236	70.35%	1231:1236	70.35%	1231:1236	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	0	20	theme	water	12:16	arg1	extraction					18:27	Subcritical water extraction	0:27	Subcritical water extraction as an efficient technique	0:53	Subcritical water extraction as an efficient technique to isolate biologically-active fucoidans from Nizamuddinia zanardinii.					
30684576	5	21	theme	monosaccharide	767:780	arg1	composition					782:792	Chemical and monosaccharide composition	754:792	composition	782:792	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	4	22	theme	Extraction	643:652	arg1	variables					695:703	the extraction variables	680:703	the extraction variables that most significantly affected fucoidan yield	680:751	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	4	22	theme	Extraction	643:652	arg1	temperature					663:673	temperature	663:673	temperature	663:673	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	4	22	theme	Extraction	643:652	arg1	time					654:657	Extraction time	643:657	Extraction time	643:657	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	0	23	theme	Subcritical	0:10	arg1	extraction					18:27	Subcritical water extraction	0:27	Subcritical water extraction as an efficient technique	0:53	Subcritical water extraction as an efficient technique to isolate biologically-active fucoidans from Nizamuddinia zanardinii.					
30684576	8	24	theme	ABTS	1206:1209	arg1	scavenging					1219:1228	appreciable ABTS radical scavenging	1194:1228	appreciable ABTS radical scavenging (70.35%)	1194:1237	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	24	theme	ABTS	1206:1209	arg1	%					1236:1236	70.35%	1231:1236	70.35%	1231:1236	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	2	25	theme	extraction	393:402	arg1	time					404:407	extraction time	393:407	extraction time of 29 min	393:417	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	5	26	theme	anticancer	834:843	arg1	activities					866:875	the antioxidant, anticancer and immunomodulatory activities	817:875	the antioxidant, anticancer and immunomodulatory activities of the extract	817:890	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	9	27	theme	anticancer	1275:1284	arg1	activity					1286:1293	The anticancer activity	1271:1293	The anticancer activity of fucoidan	1271:1305	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	8	28	theme	SCWE-extracted	1166:1179	arg1	fucoidan					1181:1188	SCWE-extracted fucoidan	1166:1188	SCWE-extracted fucoidan	1166:1188	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	1	29	theme	subcritical	216:226	arg1	SCWE					253:256	SCWE	253:256	SCWE	253:256	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	29	theme	subcritical	216:226	arg1	method					245:250	subcritical water extraction method	216:250	subcritical water extraction method (SCWE)	216:257	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	2	30	theme	150 °C	446:451	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	30	theme	150 °C	446:451	arg1	temperature					431:441	extraction temperature	420:441	extraction temperature of 150 °C	420:451	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	2	30	theme	150 °C	446:451	arg1	time					404:407	extraction time	393:407	extraction time of 29 min	393:417	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	1	31	theme	water	228:232	arg1	SCWE					253:256	SCWE	253:256	SCWE	253:256	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	31	theme	water	228:232	arg1	method					245:250	subcritical water extraction method	216:250	subcritical water extraction method (SCWE)	216:257	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	0	32	theme	efficient	35:43	arg1	technique					45:53	an efficient technique	32:53	an efficient technique	32:53	Subcritical water extraction as an efficient technique to isolate biologically-active fucoidans from Nizamuddinia zanardinii.					
30684576	1	33	theme	response	311:318	arg1	methodology					328:338	the response surface methodology	307:338	the response surface methodology	307:338	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	10	34	theme	NO	1396:1397	arg1	production					1399:1408	The NO production	1392:1408	The NO production of RAW264.7 cells	1392:1426	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	10	34	theme	NO	1396:1397	arg1	dose-dependent					1447:1460	dose-dependent	1447:1460	dose-dependent	1447:1460	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	1	35	theme	extraction	234:243	arg1	SCWE					253:256	SCWE	253:256	SCWE	253:256	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	35	theme	extraction	234:243	arg1	method					245:250	subcritical water extraction method	216:250	subcritical water extraction method (SCWE)	216:257	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	9	36	dep	46.78	1368:1372	arg1	to					1365:1366	to	1365:1366	to	1365:1366	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	8	37	theme	radical	1211:1217	arg1	scavenging					1219:1228	appreciable ABTS radical scavenging	1194:1228	appreciable ABTS radical scavenging (70.35%)	1194:1237	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	37	theme	radical	1211:1217	arg1	%					1236:1236	70.35%	1231:1236	70.35%	1231:1236	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	2	38	theme	extraction	420:429	arg1	temperature					431:441	extraction temperature	420:441	extraction temperature of 150 °C	420:451	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	4	39	theme	extraction	684:693	arg1	variables					695:703	the extraction variables	680:703	the extraction variables that most significantly affected fucoidan yield	680:751	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	4	39	theme	extraction	684:693	arg1	temperature					663:673	temperature	663:673	temperature	663:673	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	4	39	theme	extraction	684:693	arg1	time					654:657	Extraction time	643:657	Extraction time	643:657	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	1	40	theme	surface	320:326	arg1	methodology					328:338	the response surface methodology	307:338	the response surface methodology	307:338	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	3	41	theme	conventional	603:614	arg1	extraction					624:633	conventional solvent extraction	603:633	conventional solvent extraction (5.2%)	603:640	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	3	41	theme	conventional	603:614	arg1	%					639:639	5.2%	636:639	5.2%	636:639	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	10	42	from	concentration	1541:1553	arg1	34.82 μmol					1507:1516	34.82 μmol	1507:1516	34.82 μmol at a 50 μg/mL fucoidan concentration	1507:1553	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	8	43	theme	Antioxidant	1132:1142	arg1	results					1144:1150	Antioxidant results	1132:1150	Antioxidant results	1132:1150	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	7	44	theme	molecular	1076:1084	arg1	weight					1086:1091	The average molecular weight	1064:1091	The average molecular weight of the extracted fucoidan	1064:1117	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	7	44	theme	molecular	1076:1084	arg1	694 kDa					1123:1129	694 kDa	1123:1129	694 kDa	1123:1129	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	9	45	theme	fucoidan	1298:1305	arg1	activity					1286:1293	The anticancer activity	1271:1293	The anticancer activity of fucoidan	1271:1305	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	9	46	dep	49.46	1328:1332	arg1	to					1325:1326	to	1325:1326	to	1325:1326	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	10	47	theme	cells	1422:1426	arg1	production					1399:1408	The NO production	1392:1408	The NO production of RAW264.7 cells	1392:1426	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	10	47	theme	cells	1422:1426	arg1	dose-dependent					1447:1460	dose-dependent	1447:1460	dose-dependent	1447:1460	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	1	48	theme	sulfated	128:135	arg1	fucoidan					153:160	fucoidan	153:160	fucoidan	153:160	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	1	48	theme	sulfated	128:135	arg1	polysaccharide					137:150	A sulfated polysaccharide	126:150	A sulfated polysaccharide (fucoidan)	126:161	A sulfated polysaccharide (fucoidan) has been isolated from Nizamuddinia zanardinii using subcritical water extraction method (SCWE), and extraction conditions were optimised using the response surface methodology.					
30684576	2	49	theme	material-to-water	462:478	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	9	50	theme	HepG2	1379:1383	arg1	cells					1385:1389	HepG2 cells	1379:1389	HepG2 cells	1379:1389	The anticancer activity of fucoidan ranged from 24.60 to 49.46% for HeLa cells and from 23.95 to 46.78% for HepG2 cells.					
30684576	7	51	theme	extracted	1100:1108	arg1	fucoidan					1110:1117	the extracted fucoidan	1096:1117	the extracted fucoidan	1096:1117	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	10	52	theme	50 μg/mL	1523:1530	arg1	concentration					1541:1553	a 50 μg/mL fucoidan concentration	1521:1553	a 50 μg/mL fucoidan concentration	1521:1553	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	8	53	contain	had	1190:1192	arg2	scavenging					1219:1228	appreciable ABTS radical scavenging	1194:1228	appreciable ABTS radical scavenging (70.35%)	1194:1237	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	53	contain	had	1190:1192	arg2	Abs					1265:1267	0.182 Abs	1259:1267	0.182 Abs	1259:1267	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	53	contain	had	1190:1192	arg2	%					1236:1236	70.35%	1231:1236	70.35%	1231:1236	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	53	contain	had	1190:1192	arg1	fucoidan					1181:1188	SCWE-extracted fucoidan	1166:1188	SCWE-extracted fucoidan	1166:1188	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	8	53	contain	had	1190:1192	arg2	power					1252:1256	reducing power	1243:1256	reducing power (0.182 Abs)	1243:1268	Antioxidant results revealed that SCWE-extracted fucoidan had appreciable ABTS radical scavenging (70.35%) and reducing power (0.182 Abs).					
30684576	2	54	theme	extraction	353:362	arg1	conditions					364:373	The optimum extraction conditions	341:373	The optimum extraction conditions	341:373	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	10	55	theme	maximum	1469:1475	arg1	production					1480:1489	maximum NO production	1469:1489	maximum NO production	1469:1489	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	10	56	theme	RAW264.7	1413:1420	arg1	cells					1422:1426	RAW264.7 cells	1413:1426	RAW264.7 cells	1413:1426	The NO production of RAW264.7 cells was observed to be dose-dependent, while maximum NO production was found to be 34.82 μmol at a 50 μg/mL fucoidan concentration.					
30684576	2	57	theme	optimum	345:351	arg1	conditions					364:373	The optimum extraction conditions	341:373	The optimum extraction conditions	341:373	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30684576	4	58	theme	fucoidan	738:745	arg1	yield					747:751	fucoidan yield	738:751	fucoidan yield	738:751	Extraction time and temperature were the extraction variables that most significantly affected fucoidan yield.					
30684576	0	59	theme	biologically-active	66:84	arg1	fucoidans					86:94	biologically-active fucoidans	66:94	biologically-active fucoidans	66:94	Subcritical water extraction as an efficient technique to isolate biologically-active fucoidans from Nizamuddinia zanardinii.					
30684576	3	60	theme	solvent	616:622	arg1	extraction					624:633	conventional solvent extraction	603:633	conventional solvent extraction (5.2%)	603:640	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	3	60	theme	solvent	616:622	arg1	%					639:639	5.2%	636:639	5.2%	636:639	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	5	61	theme	immunomodulatory	849:864	arg1	activities					866:875	the antioxidant, anticancer and immunomodulatory activities	817:875	the antioxidant, anticancer and immunomodulatory activities of the extract	817:890	Chemical and monosaccharide composition, molecular weight, and the antioxidant, anticancer and immunomodulatory activities of the extract have also been investigated.					
30684576	3	62	theme	optimum	529:535	arg1	conditions					537:546	these optimum conditions	523:546	these optimum conditions	523:546	The fucoidan yield under these optimum conditions was 25.98%, which was considerably higher than that of conventional solvent extraction (5.2%).					
30684576	7	63	theme	fucoidan	1110:1117	arg1	weight					1086:1091	The average molecular weight	1064:1091	The average molecular weight of the extracted fucoidan	1064:1117	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	7	63	theme	fucoidan	1110:1117	arg1	694 kDa					1123:1129	694 kDa	1123:1129	694 kDa	1123:1129	The average molecular weight of the extracted fucoidan was 694 kDa.					
30684576	2	64	theme	raw	458:460	arg1	ratio					480:484	raw material-to-water ratio	458:484	raw material-to-water ratio of 21 g/mL	458:495	The optimum extraction conditions were found to be: extraction time of 29 min, extraction temperature of 150 °C, and raw material-to-water ratio of 21 g/mL.					
30292926	0	0	theme	O-antigen	70:78	arg1	unit					58:61	the repeating unit	44:61	the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	44:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	3	1	theme	reducing	760:767	arg1	stereochemistry					773:787	the reducing end stereochemistry	756:787	the reducing end stereochemistry	756:787	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	1	2	theme	Azospirillum	318:329	arg1	Jm125A2					342:348	Azospirillum brasilense Jm125A2	318:348	Azospirillum brasilense Jm125A2	318:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	3	theme	-α-L-Rha-	245:253	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	0	4	theme	Azospirillum	85:96	arg1	Jm125A2					109:115	Azospirillum brasilense Jm125A2	85:115	Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	85:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	1	5	theme	brasilense	331:340	arg1	Jm125A2					342:348	Azospirillum brasilense Jm125A2	318:348	Azospirillum brasilense Jm125A2	318:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	6	theme	linear	613:618	arg1	strategy					620:627	a linear strategy	611:627	a linear strategy	611:627	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	1	7	theme	stereoselective	460:474	arg1	glycosylations					476:489	stereoselective glycosylations	460:489	stereoselective glycosylations	460:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	3	8	theme	end	769:771	arg1	stereochemistry					773:787	the reducing end stereochemistry	756:787	the reducing end stereochemistry	756:787	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	1	9	from	-α-L-Rha-CH2CH2NH2	277:294	arg1	Jm125A2					342:348	Azospirillum brasilense Jm125A2	318:348	Azospirillum brasilense Jm125A2	318:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	10	theme	glycosylations	476:489	arg1	manipulations					400:412	rational protecting group manipulations	374:412	rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations	374:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	11	theme	1 → 3	255:259	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	12	theme	glycoside	551:559	arg1	form					526:529	the form	522:529	the form of its 2-aminoethyl glycoside	522:559	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	1	13	theme	Total	160:164	arg1	synthesis					175:183	Total chemical synthesis	160:183	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	160:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	0	14	theme	brasilense	98:107	arg1	Jm125A2					109:115	Azospirillum brasilense Jm125A2	85:115	Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	85:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	1	15	theme	chemical	166:173	arg1	synthesis					175:183	Total chemical synthesis	160:183	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	160:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	16	from	tetrasaccharide	503:517	arg1	form					526:529	the form	522:529	the form of its 2-aminoethyl glycoside	522:559	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	3	17	theme	free	727:730	arg1	amine					732:736	the terminal free amine	714:736	the terminal free amine	714:736	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	3	18	theme	glycoconjugate	681:694	arg1	formation					696:704	further glycoconjugate formation	673:704	further glycoconjugate formation	673:704	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	0	19	theme	tetrasaccharide	17:31	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the tetrasaccharide	0:31	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	1	20	theme	-α-L-Rha-	261:269	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	3	21	theme	further	673:679	arg1	formation					696:704	further glycoconjugate formation	673:704	further glycoconjugate formation	673:704	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	2	22	theme	%	579:579	arg1	yield					581:585	∼24% yield	576:585	∼24% yield	576:585	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	1	23	theme	rational	374:381	arg1	manipulations					400:412	rational protecting group manipulations	374:412	rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations	374:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	24	theme	linear	192:197	arg1	tetrasaccharide					199:213	the linear tetrasaccharide	188:213	the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	188:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	25	theme	1 → 2	271:275	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	26	theme	∼24	576:578	arg1	%					579:579	%	579:579	%	579:579	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	1	27	theme	protecting	383:392	arg1	manipulations					400:412	rational protecting group manipulations	374:412	rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations	374:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	28	theme	tetrasaccharide	199:213	arg1	synthesis					175:183	Total chemical synthesis	160:183	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	160:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	29	theme	group	394:398	arg1	manipulations					400:412	rational protecting group manipulations	374:412	rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations	374:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	30	from	Jm125A2	342:348	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	30	from	Jm125A2	342:348	arg1	O-antigen					303:311	the O-antigen	299:311	the O-antigen from Azospirillum brasilense Jm125A2	299:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	31	theme	2-aminoethyl	538:549	arg1	glycoside					551:559	its 2-aminoethyl glycoside	534:559	its 2-aminoethyl glycoside	534:559	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	0	32	theme	repeating	48:56	arg1	unit					58:61	the repeating unit	44:61	the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	44:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	0	33	theme	2-aminoethyl	136:147	arg1	glycoside					149:157	its 2-aminoethyl glycoside	132:157	its 2-aminoethyl glycoside	132:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	0	34	from	unit	58:61	arg1	Jm125A2					109:115	Azospirillum brasilense Jm125A2	85:115	Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	85:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	0	35	from	Jm125A2	109:115	arg1	form					124:127	the form	120:127	the form of its 2-aminoethyl glycoside	120:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	0	35	from	Jm125A2	109:115	arg1	unit					58:61	the repeating unit	44:61	the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	44:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	0	35	from	Jm125A2	109:115	arg1	O-antigen					70:78	the O-antigen	66:78	the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside	66:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	3	36	theme	terminal	718:725	arg1	amine					732:736	the terminal free amine	714:736	the terminal free amine	714:736	The structure is particularly suitable for further glycoconjugate formation through the terminal free amine without hampering the reducing end stereochemistry.					
30292926	1	37	theme	unit	225:228	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	0	38	theme	glycoside	149:157	arg1	form					124:127	the form	120:127	the form of its 2-aminoethyl glycoside	120:157	Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the form of its 2-aminoethyl glycoside.					
30292926	1	39	theme	available	430:438	arg1	monosaccharides					440:454	commercially available monosaccharides	417:454	commercially available monosaccharides	417:454	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	40	theme	β-D-Glc-	230:237	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	41	theme	monosaccharides	440:454	arg1	manipulations					400:412	rational protecting group manipulations	374:412	rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations	374:489	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	2	42	theme	target	496:501	arg1	tetrasaccharide					503:517	The target tetrasaccharide	492:517	The target tetrasaccharide in the form of its 2-aminoethyl glycoside	492:559	The target tetrasaccharide in the form of its 2-aminoethyl glycoside is obtained in ∼24% yield over 10 steps following a linear strategy.					
30292926	1	43	theme	O-antigen	303:311	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
30292926	1	44	theme	1 → 2	239:243	arg1	-α-L-Rha-CH2CH2NH2					277:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2	225:294	unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2	225:348	Total chemical synthesis of the linear tetrasaccharide repeating unit β-D-Glc-(1 → 2)-α-L-Rha-(1 → 3)-α-L-Rha-(1 → 2)-α-L-Rha-CH2CH2NH2 of the O-antigen from Azospirillum brasilense Jm125A2 is accomplished through rational protecting group manipulations of commercially available monosaccharides and stereoselective glycosylations.					
31883901	0	0	theme	acid	73:76	arg1	synergy					14:20	Compositional synergy	0:20	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.	0:132	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	4	1	theme	composite	1192:1200	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	2	2	theme	in-vitro	643:650	arg1	degradation					652:662	in-vitro degradation	643:662	in-vitro degradation	643:662	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	1	3	theme	response	154:161	arg1	RSM					184:186	RSM	184:186	RSM	184:186	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	3	theme	response	154:161	arg1	methodology					171:181	response surface methodology	154:181	response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	154:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	2	4	theme	independent	827:837	arg1	variables					839:847	the independent variables	823:847	the independent variables	823:847	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	0	5	theme	citric	66:71	arg1	acid					73:76	citric acid	66:76	citric acid	66:76	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	3	6	theme	St	937:938	arg1	%					935:935	10 w/w% St	929:938	10 w/w% St	929:938	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	1	7	theme	surface	163:169	arg1	RSM					184:186	RSM	184:186	RSM	184:186	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	7	theme	surface	163:169	arg1	methodology					171:181	response surface methodology	154:181	response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	154:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	8	theme	tensile	574:580	arg1	TS					592:593	TS	592:593	TS	592:593	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	8	theme	tensile	574:580	arg1	strength					582:589	tensile strength	574:589	tensile strength (TS)	574:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	9	theme	film	1434:1437	arg1	terms					1370:1374	terms	1370:1374	terms of a durable and inexpensive biocompatible wound dressing film	1370:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	3	10	theme	15 wt	955:959	arg1	Gl					962:963	15 wt% CA and 15 wt% Gl	941:963	Gl	962:963	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	4	11	theme	gram-positive	1292:1304	arg1	bacteria					1331:1338	gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1255:1338	bacteria	1331:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	11	theme	gram-positive	1292:1304	arg1	monocytogenes					1316:1328	Listeria monocytogenes	1307:1328	Listeria monocytogenes	1307:1328	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	12	theme	biocompatible	1405:1417	arg1	film					1434:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	13	theme	strength	582:589	arg1	combinations					497:508	appropriate combinations	485:508	appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	485:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	14	theme	antibacterial	1227:1239	arg1	activity					1241:1248	promising antibacterial activity	1217:1248	promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1217:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	2	15	theme	quadratic	673:681	arg1	expressions					683:693	quadratic expressions	673:693	quadratic expressions	673:693	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	3	16	theme	%	960:960	arg1	Gl					962:963	15 wt% CA and 15 wt% Gl	941:963	Gl	962:963	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	4	17	theme	inexpensive	1393:1403	arg1	film					1434:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	18	theme	appropriate	485:495	arg1	combinations					497:508	appropriate combinations	485:508	appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	485:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	19	theme	promising	1217:1225	arg1	activity					1241:1248	promising antibacterial activity	1217:1248	promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1217:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	0	20	theme	dressing	106:113	arg1	fabrication					121:131	wound dressing films fabrication	100:131	wound dressing films fabrication	100:131	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	1	21	theme	polyvinyl	365:373	arg1	%					442:442	15-40 wt%	434:442	15-40 wt%	434:442	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	21	theme	polyvinyl	365:373	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (5-10 w/w%)	365:393	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	21	theme	polyvinyl	365:373	arg1	%					392:392	5-10 w/w%	384:392	5-10 w/w%	384:392	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	21	theme	polyvinyl	365:373	arg1	compatibility					250:262	the compatibility	246:262	the compatibility	246:262	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	22	theme	dressing	1425:1432	arg1	film					1434:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	23	from	competence	1356:1365	arg1	terms					1370:1374	terms	1370:1374	terms of a durable and inexpensive biocompatible wound dressing film	1370:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	3	24	theme	water	1093:1097	arg1	solubility					1099:1108	water solubility	1093:1108	water solubility	1093:1108	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	0	25	theme	wound	100:104	arg1	fabrication					121:131	wound dressing films fabrication	100:131	wound dressing films fabrication	100:131	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	4	26	theme	polymer	1184:1190	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	27	theme	wound	1419:1423	arg1	film					1434:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	3	28	dep	composition	904:914	arg1	Gl					962:963	15 wt% CA and 15 wt% Gl	941:963	Gl	962:963	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	28	dep	composition	904:914	arg1	PVA					924:926	5 w/w% PVA	917:926	5 w/w% PVA	917:926	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	28	dep	composition	904:914	arg1	CA					948:949	15 wt% CA and 15 wt% Gl	941:963	CA	948:949	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	28	dep	composition	904:914	arg1	%					935:935	10 w/w% St	929:938	10 w/w% St	929:938	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	29	theme	gel	1045:1047	arg1	fraction					1049:1056	gel fraction	1045:1056	gel fraction (GF 34%)	1045:1065	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	29	theme	gel	1045:1047	arg1	%					1064:1064	GF 34%	1059:1064	GF 34%	1059:1064	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	2	30	theme	variables	839:847	arg1	terms					814:818	bilinear and linear terms	794:818	bilinear and linear terms of the independent variables	794:847	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	1	31	theme	15-40 wt	434:441	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (5-10 w/w%)	365:393	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	31	theme	15-40 wt	434:441	arg1	%					442:442	15-40 wt%	434:442	15-40 wt%	434:442	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	32	theme	swelling	513:520	arg1	SI					529:530	SI	529:530	SI	529:530	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	32	theme	swelling	513:520	arg1	index					522:526	swelling index	513:526	swelling index (SI)	513:531	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	0	33	theme	Compositional	0:12	arg1	synergy					14:20	Compositional synergy	0:20	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.	0:132	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	4	34	theme	based	1178:1182	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	35	dep	compatibility	250:262	arg1	concentrations					327:340	[variant macromolecular concentrations	303:340	[variant macromolecular concentrations of starch	303:350	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	2	36	theme	design	601:606	arg1	studies					608:614	The design studies	597:614	The design studies	597:614	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	1	37	theme	index	522:526	arg1	combinations					497:508	appropriate combinations	485:508	appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	485:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	38	theme	Listeria	1307:1314	arg1	bacteria					1331:1338	gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1255:1338	bacteria	1331:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	38	theme	Listeria	1307:1314	arg1	monocytogenes					1316:1328	Listeria monocytogenes	1307:1328	Listeria monocytogenes	1307:1328	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	0	39	theme	poly-vinyl	25:34	arg1	alcohol					36:42	poly-vinyl alcohol	25:42	poly-vinyl alcohol	25:42	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	1	40	theme	quaternary	279:288	arg1	formulations					290:301	quaternary formulations	279:301	quaternary formulations	279:301	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	41	theme	%	946:946	arg1	CA					948:949	15 wt% CA and 15 wt% Gl	941:963	CA	948:949	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	42	theme	optimized	888:896	arg1	film					898:901	optimized film	888:901	optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl)	888:964	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	1	43	theme	formulations	290:301	arg1	glycerol					424:431	glycerol	424:431	glycerol	424:431	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	43	theme	formulations	290:301	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (5-10 w/w%)	365:393	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	43	theme	formulations	290:301	arg1	acid					403:406	citric acid	396:406	citric acid (15-40 wt%)	396:418	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	43	theme	formulations	290:301	arg1	%					361:361	5-10 w/w%	353:361	5-10 w/w%	353:361	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	43	theme	formulations	290:301	arg1	compatibility					250:262	the compatibility	246:262	the compatibility	246:262	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	43	theme	formulations	290:301	arg1	synergy					268:274	synergy	268:274	synergy of quaternary formulations	268:301	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	0	44	theme	films	115:119	arg1	fabrication					121:131	wound dressing films fabrication	100:131	wound dressing films fabrication	100:131	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	4	45	theme	durable	1381:1387	arg1	film					1434:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	a durable and inexpensive biocompatible wound dressing film	1379:1437	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	3	46	theme	experimental	859:870	arg1	investigations					872:885	experimental investigations	859:885	experimental investigations	859:885	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	2	47	theme	quaternary	729:738	arg1	compositions					740:751	the quaternary compositions	725:751	the quaternary compositions	725:751	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	4	48	with	activity	1241:1248	arg1	gram-negative					1255:1267	gram-negative	1255:1267	gram-negative	1255:1267	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	48	with	activity	1241:1248	arg1	bacteria					1331:1338	gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1255:1338	bacteria	1331:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	48	with	activity	1241:1248	arg1	monocytogenes					1316:1328	Listeria monocytogenes	1307:1328	Listeria monocytogenes	1307:1328	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	49	theme	[variant	303:310	arg1	concentrations					327:340	[variant macromolecular concentrations	303:340	[variant macromolecular concentrations of starch	303:350	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	50	theme	%	922:922	arg1	PVA					924:926	5 w/w% PVA	917:926	5 w/w% PVA	917:926	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	0	51	theme	alcohol	36:42	arg1	synergy					14:20	Compositional synergy	0:20	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.	0:132	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	1	52	theme	macromolecular	312:325	arg1	concentrations					327:340	[variant macromolecular concentrations	303:340	[variant macromolecular concentrations of starch	303:350	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	53	theme	higher	1068:1073	arg1	TS					1075:1076	higher TS	1068:1076	higher TS (7.65 MPa)	1068:1087	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	53	theme	higher	1068:1073	arg1	%					1113:1113	66%	1111:1113	66%	1111:1113	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	53	theme	higher	1068:1073	arg1	7.65 MPa					1079:1086	7.65 MPa	1079:1086	7.65 MPa	1079:1086	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	54	theme	excellent	976:984	arg1	SI					986:987	excellent SI	976:987	excellent SI (338.37%)	976:997	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	54	theme	excellent	976:984	arg1	%					996:996	338.37%	990:996	338.37%	990:996	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	1	55	from	insights	234:241	arg1	glycerol					424:431	glycerol	424:431	glycerol	424:431	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	55	from	insights	234:241	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (5-10 w/w%)	365:393	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	55	from	insights	234:241	arg1	acid					403:406	citric acid	396:406	citric acid (15-40 wt%)	396:418	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	55	from	insights	234:241	arg1	%					361:361	5-10 w/w%	353:361	5-10 w/w%	353:361	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	55	from	insights	234:241	arg1	compatibility					250:262	the compatibility	246:262	the compatibility	246:262	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	55	from	insights	234:241	arg1	synergy					268:274	synergy	268:274	synergy of quaternary formulations	268:301	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	4	56	theme	composition	1166:1176	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	57	dep	methodology	171:181	arg1	design					189:194	design	189:194	response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	154:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	58	theme	acceptable	1000:1009	arg1	degradation					1020:1030	acceptable in-vitro degradation	1000:1030	acceptable in-vitro degradation (53.27%)	1000:1039	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	58	theme	acceptable	1000:1009	arg1	%					1038:1038	53.27%	1033:1038	53.27%	1033:1038	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	0	59	theme	starch	45:50	arg1	synergy					14:20	Compositional synergy	0:20	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.	0:132	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	4	60	theme	optimal	1158:1164	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	61	theme	citric	396:401	arg1	acid					403:406	citric acid	396:406	citric acid (15-40 wt%)	396:418	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	61	theme	citric	396:401	arg1	%					417:417	15-40 wt%	409:417	15-40 wt%	409:417	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	61	theme	citric	396:401	arg1	compatibility					250:262	the compatibility	246:262	the compatibility	246:262	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	0	62	dep	alcohol	36:42	arg1	concentrations					78:91	concentrations	78:91	concentrations	78:91	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	3	63	dep	film	898:901	arg1	composition					904:914	composition	904:914	composition	904:914	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	1	64	theme	starch	345:350	arg1	concentrations					327:340	[variant macromolecular concentrations	303:340	[variant macromolecular concentrations of starch	303:350	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	2	65	with	complex	781:787	arg1	terms					814:818	bilinear and linear terms	794:818	bilinear and linear terms of the independent variables	794:847	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	4	66	dep	gram-negative	1255:1267	arg1	coli					1282:1285	Escherichia coli	1270:1285	Escherichia coli	1270:1285	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	67	contain	possessed	1207:1215	arg1	film					1202:1205	the optimal composition based polymer composite film	1154:1205	the optimal composition based polymer composite film	1154:1205	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	4	67	contain	possessed	1207:1215	arg2	activity					1241:1248	promising antibacterial activity	1217:1248	promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria	1217:1338	Further, the optimal composition based polymer composite film possessed promising antibacterial activity with gram-negative (Escherichia coli) and gram-positive (Listeria monocytogenes) bacteria to indicate its competence in terms of a durable and inexpensive biocompatible wound dressing film.					
31883901	1	68	theme	in-vitro	534:541	arg1	degradation					543:553	in-vitro degradation	534:553	in-vitro degradation during 27 days	534:568	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	69	with	films	474:478	arg1	combinations					497:508	appropriate combinations	485:508	appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	485:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	70	theme	in-vitro	1011:1018	arg1	degradation					1020:1030	acceptable in-vitro degradation	1000:1030	acceptable in-vitro degradation (53.27%)	1000:1039	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	70	theme	in-vitro	1011:1018	arg1	%					1038:1038	53.27%	1033:1038	53.27%	1033:1038	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	2	71	from	variations	711:720	arg1	compositions					740:751	the quaternary compositions	725:751	the quaternary compositions	725:751	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	1	72	theme	wound	459:463	arg1	films					474:478	wound dressing films	459:478	wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	459:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	73	theme	poor	1120:1123	arg1	elongation					1125:1134	poor elongation	1120:1134	poor elongation (9.13%)	1120:1142	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	73	theme	poor	1120:1123	arg1	%					1141:1141	9.13%	1137:1141	9.13%	1137:1141	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	1	74	theme	degradation	543:553	arg1	combinations					497:508	appropriate combinations	485:508	appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	485:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	75	theme	useful	227:232	arg1	insights					234:241	useful insights	227:241	useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)]	227:444	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	76	theme	15-40 wt	409:416	arg1	acid					403:406	citric acid	396:406	citric acid (15-40 wt%)	396:418	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	76	theme	15-40 wt	409:416	arg1	%					417:417	15-40 wt%	409:417	15-40 wt%	409:417	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	1	77	theme	dressing	465:472	arg1	films					474:478	wound dressing films	459:478	wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS)	459:594	This work addresses response surface methodology (RSM) design based investigations to obtain useful insights on the compatibility and synergy of quaternary formulations [variant macromolecular concentrations of starch (5-10 w/w%), polyvinyl alcohol (5-10 w/w%), citric acid (15-40 wt%) and glycerol (15-40 wt%)] and to yield wound dressing films with appropriate combinations of swelling index (SI), in-vitro degradation during 27 days and tensile strength (TS).					
31883901	3	78	theme	15 wt	941:945	arg1	CA					948:949	15 wt% CA and 15 wt% Gl	941:963	CA	948:949	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	2	79	theme	bilinear	794:801	arg1	terms					814:818	bilinear and linear terms	794:818	bilinear and linear terms of the independent variables	794:847	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	0	80	theme	glycerol	53:60	arg1	synergy					14:20	Compositional synergy	0:20	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.	0:132	Compositional synergy of poly-vinyl alcohol, starch, glycerol and citric acid concentrations during wound dressing films fabrication.					
31883901	3	81	theme	5 w/w	917:921	arg1	PVA					924:926	5 w/w% PVA	917:926	5 w/w% PVA	917:926	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	2	82	theme	linear	807:812	arg1	terms					814:818	bilinear and linear terms	794:818	bilinear and linear terms of the independent variables	794:847	The design studies inferred that while SI and in-vitro degradation followed quadratic expressions with respect to variations in the quaternary compositions, the TS was relatively less complex with bilinear and linear terms of the independent variables.					
31883901	3	83	theme	GF	1059:1060	arg1	fraction					1049:1056	gel fraction	1045:1056	gel fraction (GF 34%)	1045:1065	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31883901	3	83	theme	GF	1059:1060	arg1	%					1064:1064	GF 34%	1059:1064	GF 34%	1059:1064	Based on experimental investigations, optimized film (composition: 5 w/w% PVA, 10 w/w% St, 15 wt% CA and 15 wt% Gl) exhibited excellent SI (338.37%), acceptable in-vitro degradation (53.27%) and gel fraction (GF 34%), higher TS (7.65 MPa) and water solubility (66%) but poor elongation (9.13%).					
31689590	0	0	theme	strawberry	93:102	arg1	fruit					104:108	strawberry fruit	93:108	strawberry fruit	93:108	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	1	from	identification	14:27	arg1	arrhenomanes					61:72	Pythium arrhenomanes	53:72	Pythium arrhenomanes	53:72	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	6	2	theme	greater	745:751	arg1	activity					777:784	greater DPPH radical-scavenging activity	745:784	greater DPPH radical-scavenging activity	745:784	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	3	from	activity	777:784	arg1	index					634:638	decay index	628:638	decay index	628:638	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	3	from	activity	777:784	arg1	malondialdehyde					650:664	lower malondialdehyde	644:664	lower malondialdehyde	644:664	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	4	from	delay	619:623	arg1	index					634:638	decay index	628:638	decay index	628:638	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	4	from	delay	619:623	arg1	malondialdehyde					650:664	lower malondialdehyde	644:664	lower malondialdehyde	644:664	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	0	5	from	Isolation	0:8	arg1	arrhenomanes					61:72	Pythium arrhenomanes	53:72	Pythium arrhenomanes	53:72	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	5	6	theme	polysaccharide-based	440:459	arg1	PEPS-2					417:422	PEPS-2	417:422	PEPS-2	417:422	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	5	6	theme	polysaccharide-based	440:459	arg1	coating					461:467	a polysaccharide-based coating	438:467	a polysaccharide-based coating for strawberry fruit	438:488	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	0	7	from	arrhenomanes	61:72	arg1	identification					14:27	identification	14:27	identification	14:27	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	7	from	arrhenomanes	61:72	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	7	from	arrhenomanes	61:72	arg1	Duch					129:132	Duch	129:132	Duch	129:132	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	7	from	arrhenomanes	61:72	arg1	application					78:88	application	78:88	application to strawberry fruit	78:108	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	7	from	arrhenomanes	61:72	arg1	polysaccharides					32:46	polysaccharides	32:46	polysaccharides from Pythium arrhenomanes	32:72	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	5	8	theme	postharvest	510:520	arg1	preservation					522:533	postharvest preservation	510:533	postharvest preservation of the fruit	510:546	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	2	9	dep	polysaccharides	257:271	arg1	PEPS-3					290:295	PEPS-3	290:295	PEPS-3	290:295	In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3).					
31689590	2	9	dep	polysaccharides	257:271	arg1	PEPS-2					282:287	PEPS-2	282:287	PEPS-2	282:287	In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3).					
31689590	2	9	dep	polysaccharides	257:271	arg1	PEPS-1					274:279	PEPS-1	274:279	PEPS-1	274:279	In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3).					
31689590	7	10	theme	strawberries	888:899	arg1	shelf-life					874:883	the shelf-life	870:883	the shelf-life of strawberries	870:899	The developed method could be beneficial for improving the shelf-life of strawberries.					
31689590	6	11	theme	C	730:730	arg1	content					732:738	vitamin C content	722:738	vitamin C content	722:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	11	theme	C	730:730	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	4	12	theme	antioxidant	393:403	arg1	activities					405:414	outstanding antioxidant activities	381:414	outstanding antioxidant activities	381:414	PEPS-2 and PEPS-3 showed outstanding antioxidant activities.					
31689590	6	13	theme	vitamin	722:728	arg1	content					732:738	vitamin C content	722:738	vitamin C content	722:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	13	theme	vitamin	722:728	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	14	theme	decay	628:632	arg1	index					634:638	decay index	628:638	decay index	628:638	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	4	15	theme	outstanding	381:391	arg1	activities					405:414	outstanding antioxidant activities	381:414	outstanding antioxidant activities	381:414	PEPS-2 and PEPS-3 showed outstanding antioxidant activities.					
31689590	6	16	theme	radical-scavenging	758:775	arg1	activity					777:784	greater DPPH radical-scavenging activity	745:784	greater DPPH radical-scavenging activity	745:784	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	17	from	solids	682:687	arg1	index					634:638	decay index	628:638	decay index	628:638	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	17	from	solids	682:687	arg1	malondialdehyde					650:664	lower malondialdehyde	644:664	lower malondialdehyde	644:664	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	18	theme	anthocyanin	698:708	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	18	theme	anthocyanin	698:708	arg1	content					710:716	anthocyanin content	698:716	anthocyanin content	698:716	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	0	19	theme	polysaccharides	32:46	arg1	identification					14:27	identification	14:27	identification	14:27	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	19	theme	polysaccharides	32:46	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	19	theme	polysaccharides	32:46	arg1	Duch					129:132	Duch	129:132	Duch	129:132	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	0	19	theme	polysaccharides	32:46	arg1	application					78:88	application	78:88	application to strawberry fruit	78:108	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	7	20	theme	developed	819:827	arg1	method					829:834	The developed method	815:834	The developed method	815:834	The developed method could be beneficial for improving the shelf-life of strawberries.					
31689590	7	20	theme	developed	819:827	arg1	beneficial					845:854	beneficial	845:854	beneficial	845:854	The developed method could be beneficial for improving the shelf-life of strawberries.					
31689590	6	21	theme	significant	607:617	arg1	delay					619:623	significant delay	607:623	significant delay in decay index and lower malondialdehyde	607:664	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	0	22	from	application	78:88	arg1	arrhenomanes					61:72	Pythium arrhenomanes	53:72	Pythium arrhenomanes	53:72	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	5	23	from	effects	499:505	arg1	preservation					522:533	postharvest preservation	510:533	postharvest preservation of the fruit	510:546	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	6	24	theme	control	800:806	arg1	fruit					808:812	uncoated control fruit	791:812	uncoated control fruit	791:812	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	0	25	theme	Pythium	53:59	arg1	arrhenomanes					61:72	Pythium arrhenomanes	53:72	Pythium arrhenomanes	53:72	Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.)					
31689590	6	26	theme	uncoated	791:798	arg1	fruit					808:812	uncoated control fruit	791:812	uncoated control fruit	791:812	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	2	27	theme	Pythium	202:208	arg1	arrhenomanes					210:221	Pythium arrhenomanes	202:221	Pythium arrhenomanes	202:221	In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3).					
31689590	6	28	dep	solids	682:687	arg1	content					732:738	vitamin C content	722:738	vitamin C content	722:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	28	dep	solids	682:687	arg1	content					689:695	content	689:695	content	689:695	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	28	dep	solids	682:687	arg1	content					710:716	anthocyanin content	698:716	anthocyanin content	698:716	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	28	dep	solids	682:687	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	5	29	theme	strawberry	473:482	arg1	fruit					484:488	strawberry fruit	473:488	strawberry fruit	473:488	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	3	30	theme	polysaccharides	324:338	arg1	composition					303:313	The composition	299:313	The composition of these polysaccharides	299:338	The composition of these polysaccharides was determined.					
31689590	6	31	theme	lower	644:648	arg1	malondialdehyde					650:664	lower malondialdehyde	644:664	lower malondialdehyde	644:664	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	5	32	theme	fruit	542:546	arg1	preservation					522:533	postharvest preservation	510:533	postharvest preservation of the fruit	510:546	PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined.					
31689590	6	33	theme	soluble	674:680	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	33	theme	soluble	674:680	arg1	content					689:695	content	689:695	content	689:695	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	33	theme	soluble	674:680	arg1	content					710:716	anthocyanin content	698:716	anthocyanin content	698:716	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	33	theme	soluble	674:680	arg1	content					732:738	vitamin C content	722:738	vitamin C content	722:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	34	theme	DPPH	753:756	arg1	activity					777:784	greater DPPH radical-scavenging activity	745:784	greater DPPH radical-scavenging activity	745:784	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	35	theme	higher	667:672	arg1	solids					682:687	higher soluble solids	667:687	higher soluble solids content, anthocyanin content and vitamin C content	667:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	35	theme	higher	667:672	arg1	content					689:695	content	689:695	content	689:695	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	35	theme	higher	667:672	arg1	content					710:716	anthocyanin content	698:716	anthocyanin content	698:716	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
31689590	6	35	theme	higher	667:672	arg1	content					732:738	vitamin C content	722:738	vitamin C content	722:738	Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit.					
30892132	2	0	from	effects	384:390	arg1	interaction					424:434	mAb interaction	420:434	mAb interaction	420:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	6	1	theme	toripalimab	1226:1236	arg1	mechanisms					1212:1221	the binding mechanisms	1200:1221	the binding mechanisms of toripalimab to PD-1	1200:1244	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	1	2	theme	-based	150:155	arg1	blockade					157:164	Monoclonal antibody (mAb)-based blockade	125:164	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity	125:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	4	3	theme	structural	957:966	arg1	evidences					968:976	structural evidences	957:976	structural evidences	957:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	0	4	theme	PD-1	83:86	arg1	loop					75:78	FG loop	72:78	FG loop of PD-1 for tumor immune checkpoint therapy	72:122	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	5	5	theme	N-linked	1049:1056	arg1	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	4	6	theme	FG	776:777	arg1	loop					779:782	the FG loop	772:782	the FG loop of PD-1	772:790	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	5	7	located	observed	1021:1028	arg1	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	5	7	located	observed	1021:1028	arg2	modifications					990:1002	The glycan modifications	979:1002	The glycan modifications of PD-1	979:1010	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	1	8	theme	antitumor	224:232	arg1	immunity					241:248	antitumor T-cell immunity	224:248	antitumor T-cell immunity	224:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	0	9	theme	immune	98:103	arg1	therapy					116:122	tumor immune checkpoint therapy	92:122	tumor immune checkpoint therapy	92:122	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	5	10	theme	glycosylation	1058:1070	arg1	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	6	11	theme	binding	1204:1210	arg1	mechanisms					1212:1221	the binding mechanisms	1200:1221	the binding mechanisms of toripalimab to PD-1	1200:1244	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	4	12	with	mAbs	947:950	arg1	evidences					968:976	structural evidences	957:976	structural evidences	957:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	3	13	theme	complex	481:487	arg1	structure					489:497	the complex structure	477:497	the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US	477:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	1	14	theme	T-cell	234:239	arg1	immunity					241:248	antitumor T-cell immunity	224:248	antitumor T-cell immunity	224:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	0	15	theme	tumor	92:96	arg1	therapy					116:122	tumor immune checkpoint therapy	92:122	tumor immune checkpoint therapy	92:122	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	3	16	theme	Phase	660:664	arg1	trials					685:690	multiple Phase 1-Phase 3 clinical trials	651:690	multiple Phase 1-Phase 3 clinical trials in both China and the US	651:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	4	17	theme	PD-1	787:790	arg1	loop					779:782	the FG loop	772:782	the FG loop of PD-1	772:790	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	7	18	theme	Protein	1359:1365	arg1	Bank					1372:1375	the Protein Data Bank	1355:1375	the Protein Data Bank	1355:1375	Atomic coordinates have been deposited in the Protein Data Bank under accession code 6JBT.					
30892132	3	19	theme	1-Phase	666:672	arg1	trials					685:690	multiple Phase 1-Phase 3 clinical trials	651:690	multiple Phase 1-Phase 3 clinical trials in both China and the US	651:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	2	20	theme	mAbs	362:365	arg1	PD-1					371:374	PD-1	371:374	PD-1	371:374	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	20	theme	mAbs	362:365	arg1	mechanisms					344:353	the binding mechanisms	332:353	the binding mechanisms of the mAbs	332:365	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	0	21	theme	checkpoint	105:114	arg1	therapy					116:122	tumor immune checkpoint therapy	92:122	tumor immune checkpoint therapy	92:122	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	4	22	theme	known	911:915	arg1	epitopes					925:932	the known binding epitopes	907:932	the known binding epitopes of anti-PD-1 mAbs with structural evidences	907:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	2	23	theme	glycosylation	395:407	arg1	effects					384:390	the effects	380:390	the effects of glycosylation of PD-1 on mAb interaction	380:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	23	theme	glycosylation	395:407	arg1	basis					323:327	the structural basis	308:327	the structural basis of the binding mechanisms of the mAbs and PD-1	308:374	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	5	24	gly	glycosylation	1058:1070	arg2	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	5	24	gly	glycosylation	1058:1070	arg2	three					1033:1037	three	1033:1037	three	1033:1037	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	1	25	theme	programmed	169:178	arg1	death					185:189	programmed cell death 1	169:191	programmed cell death 1 (PD-1)	169:198	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	1	25	theme	programmed	169:178	arg1	PD-1					194:197	PD-1	194:197	PD-1	194:197	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	4	26	theme	anti-PD-1	937:945	arg1	mAbs					947:950	anti-PD-1 mAbs	937:950	anti-PD-1 mAbs with structural evidences	937:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	3	27	theme	multiple	651:658	arg1	trials					685:690	multiple Phase 1-Phase 3 clinical trials	651:690	multiple Phase 1-Phase 3 clinical trials in both China and the US	651:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	28	from	trials	685:690	arg1	China					700:704	China	700:704	China	700:704	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	28	from	trials	685:690	arg1	US					714:715	US	714:715	US	714:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	2	29	from	basis	323:327	arg1	interaction					424:434	mAb interaction	420:434	mAb interaction	420:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	4	30	theme	heavy	871:875	arg1	chain					877:881	the heavy chain	867:881	the heavy chain	867:881	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	1	31	theme	cell	180:183	arg1	death					185:189	programmed cell death 1	169:191	programmed cell death 1 (PD-1)	169:198	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	1	31	theme	cell	180:183	arg1	PD-1					194:197	PD-1	194:197	PD-1	194:197	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	0	32	theme	Glycosylation-independent	0:24	arg1	binding					26:32	Glycosylation-independent binding	0:32	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.	0:123	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	5	33	theme	substantial	1088:1098	arg1	influences					1100:1109	no substantial influences	1085:1109	no substantial influences	1085:1109	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	5	34	link	N-linked	1049:1056	arg1	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	1	35	theme	death	185:189	arg1	blockade					157:164	Monoclonal antibody (mAb)-based blockade	125:164	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity	125:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	0	36	theme	monoclonal	37:46	arg1	toripalimab					57:67	monoclonal antibody toripalimab	37:67	monoclonal antibody toripalimab	37:67	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	5	37	theme	PD-1	1007:1010	arg1	modifications					990:1002	The glycan modifications	979:1002	The glycan modifications of PD-1	979:1010	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	6	38	theme	mechanisms	1212:1221	arg1	understanding					1183:1195	our understanding	1179:1195	our understanding of the binding mechanisms of toripalimab to PD-1	1179:1244	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	6	39	theme	PD-1	1307:1310	arg1	development					1272:1282	future development	1265:1282	future development of biologics targeting PD-1	1265:1310	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	4	40	theme	mAbs	947:950	arg1	epitopes					925:932	the known binding epitopes	907:932	the known binding epitopes of anti-PD-1 mAbs with structural evidences	907:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	5	41	theme	potential	1039:1047	arg1	sites					1072:1076	three potential N-linked glycosylation sites	1033:1076	three potential N-linked glycosylation sites	1033:1076	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	5	42	theme	glycan	983:988	arg1	modifications					990:1002	The glycan modifications	979:1002	The glycan modifications of PD-1	979:1010	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	3	43	theme	PD-1	502:505	arg1	structure					489:497	the complex structure	477:497	the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US	477:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	44	with	structure	489:497	arg1	mAb					527:529	a mAb	525:529	a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US	525:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	44	with	structure	489:497	arg1	approved					539:546	approved	539:546	approved	539:546	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	44	with	structure	489:497	arg1	toripalimab					512:522	toripalimab	512:522	toripalimab	512:522	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	4	45	dep	region	850:855	arg1	loop					859:862	3 loop	857:862	an unconventionally long complementarity-determining region 3 loop	797:862	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	0	46	theme	toripalimab	57:67	arg1	binding					26:32	Glycosylation-independent binding	0:32	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.	0:123	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	3	47	theme	clinical	676:683	arg1	trials					685:690	multiple Phase 1-Phase 3 clinical trials	651:690	multiple Phase 1-Phase 3 clinical trials in both China and the US	651:715	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	7	48	theme	Atomic	1313:1318	arg1	coordinates					1320:1330	Atomic coordinates	1313:1330	Atomic coordinates	1313:1330	Atomic coordinates have been deposited in the Protein Data Bank under accession code 6JBT.					
30892132	2	49	theme	mechanisms	344:353	arg1	effects					384:390	the effects	380:390	the effects of glycosylation of PD-1 on mAb interaction	380:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	49	theme	mechanisms	344:353	arg1	basis					323:327	the structural basis	308:327	the structural basis of the binding mechanisms of the mAbs and PD-1	308:374	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	50	theme	PD-1	371:374	arg1	effects					384:390	the effects	380:390	the effects of glycosylation of PD-1 on mAb interaction	380:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	50	theme	PD-1	371:374	arg1	basis					323:327	the structural basis	308:327	the structural basis of the binding mechanisms of the mAbs and PD-1	308:374	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	51	theme	mAb	420:422	arg1	interaction					424:434	mAb interaction	420:434	mAb interaction	420:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	4	52	theme	binding	917:923	arg1	epitopes					925:932	the known binding epitopes	907:932	the known binding epitopes of anti-PD-1 mAbs with structural evidences	907:976	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	0	53	theme	antibody	48:55	arg1	toripalimab					57:67	monoclonal antibody toripalimab	37:67	monoclonal antibody toripalimab	37:67	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	3	54	theme	National	557:564	arg1	Products					574:581	China National Medical Products	551:581	China National Medical Products Administration as a second-line treatment for melanoma	551:636	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	3	55	dep	Products	574:581	arg1	Administration					583:596	Administration	583:596	Administration	583:596	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	4	56	theme	chain	877:881	arg1	distinct					893:900	distinct	893:900	distinct	893:900	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	4	56	theme	chain	877:881	arg1	region					850:855	an unconventionally long complementarity-determining region	797:855	an unconventionally long complementarity-determining region 3 loop	797:862	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	0	57	theme	FG	72:73	arg1	loop					75:78	FG loop	72:78	FG loop of PD-1 for tumor immune checkpoint therapy	72:122	Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy.					
30892132	1	58	theme	multiple	282:289	arg1	tumors					291:296	multiple tumors	282:296	multiple tumors	282:296	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	6	59	theme	targeting	1297:1305	arg1	PD-1					1307:1310	biologics targeting PD-1	1287:1310	biologics targeting PD-1	1287:1310	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	3	60	theme	Medical	566:572	arg1	Products					574:581	China National Medical Products	551:581	China National Medical Products Administration as a second-line treatment for melanoma	551:636	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	2	61	theme	structural	312:321	arg1	basis					323:327	the structural basis	308:327	the structural basis of the binding mechanisms of the mAbs and PD-1	308:374	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	62	theme	PD-1	412:415	arg1	glycosylation					395:407	glycosylation	395:407	glycosylation of PD-1	395:415	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	6	63	theme	future	1265:1270	arg1	development					1272:1282	future development	1265:1282	future development of biologics targeting PD-1	1265:1310	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
30892132	3	64	theme	second-line	603:613	arg1	treatment					615:623	a second-line treatment	601:623	a second-line treatment for melanoma	601:636	Here, we report the complex structure of PD-1 with toripalimab, a mAb that is approved by China National Medical Products Administration as a second-line treatment for melanoma and is under multiple Phase 1-Phase 3 clinical trials in both China and the US.					
30892132	4	65	from	epitopes	925:932	arg1	distinct					893:900	distinct	893:900	distinct	893:900	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	4	65	from	epitopes	925:932	arg1	region					850:855	an unconventionally long complementarity-determining region	797:855	an unconventionally long complementarity-determining region 3 loop	797:862	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	1	66	theme	Monoclonal	125:134	arg1	antibody					136:143	Monoclonal antibody	125:143	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity	125:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	1	66	theme	Monoclonal	125:134	arg1	mAb					146:148	mAb	146:148	mAb	146:148	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	7	67	dep	6JBT	1398:1401	arg1	code					1393:1396	code	1393:1396	code	1393:1396	Atomic coordinates have been deposited in the Protein Data Bank under accession code 6JBT.					
30892132	4	68	theme	complementarity-determining	822:848	arg1	distinct					893:900	distinct	893:900	distinct	893:900	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	4	68	theme	complementarity-determining	822:848	arg1	region					850:855	an unconventionally long complementarity-determining region	797:855	an unconventionally long complementarity-determining region 3 loop	797:862	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	2	69	gly	glycosylation	395:407	arg1	mAb					420:422	mAb interaction	420:434	mAb interaction	420:434	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	2	69	gly	glycosylation	395:407	arg1	PD-1					412:415	PD-1	412:415	PD-1	412:415	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	1	70	theme	antibody	136:143	arg1	blockade					157:164	Monoclonal antibody (mAb)-based blockade	125:164	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity	125:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	5	71	theme	toripalimab	1143:1153	arg1	binding					1132:1138	the binding	1128:1138	the binding of toripalimab	1128:1153	The glycan modifications of PD-1 could be observed in three potential N-linked glycosylation sites, while no substantial influences were detected to the binding of toripalimab.					
30892132	4	72	theme	long	817:820	arg1	distinct					893:900	distinct	893:900	distinct	893:900	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	4	72	theme	long	817:820	arg1	region					850:855	an unconventionally long complementarity-determining region	797:855	an unconventionally long complementarity-determining region 3 loop	797:862	Our analysis reveals that toripalimab mainly binds to the FG loop of PD-1 with an unconventionally long complementarity-determining region 3 loop of the heavy chain, which is distinct from the known binding epitopes of anti-PD-1 mAbs with structural evidences.					
30892132	7	73	theme	Data	1367:1370	arg1	Bank					1372:1375	the Protein Data Bank	1355:1375	the Protein Data Bank	1355:1375	Atomic coordinates have been deposited in the Protein Data Bank under accession code 6JBT.					
30892132	2	74	theme	binding	336:342	arg1	mechanisms					344:353	the binding mechanisms	332:353	the binding mechanisms of the mAbs	332:365	However, the structural basis of the binding mechanisms of the mAbs and PD-1 and the effects of glycosylation of PD-1 on mAb interaction are not well understood.					
30892132	1	75	theme	ligand	207:212	arg1	blockade					157:164	Monoclonal antibody (mAb)-based blockade	125:164	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity	125:248	Monoclonal antibody (mAb)-based blockade of programmed cell death 1 (PD-1) or its ligand to enable antitumor T-cell immunity has been successful in treating multiple tumors.					
30892132	7	76	theme	accession	1383:1391	arg1	6JBT					1398:1401	accession code 6JBT	1383:1401	accession code 6JBT	1383:1401	Atomic coordinates have been deposited in the Protein Data Bank under accession code 6JBT.					
30892132	6	77	theme	biologics	1287:1295	arg1	PD-1					1307:1310	biologics targeting PD-1	1287:1310	biologics targeting PD-1	1287:1310	These findings benefit our understanding of the binding mechanisms of toripalimab to PD-1 and shed light for future development of biologics targeting PD-1.					
31227151	4	0	theme	chain	812:816	arg1	arabinose					818:826	side chain arabinose	807:826	side chain arabinose	807:826	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	3	1	theme	Chemical	475:482	arg1	composition					484:494	Chemical composition	475:494	Chemical composition	475:494	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	2	theme	TGA	650:652	arg1	techniques					654:663	TGA techniques	650:663	TGA techniques	650:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	4	3	theme	side	807:810	arg1	arabinose					818:826	side chain arabinose	807:826	side chain arabinose	807:826	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	6	4	theme	P.	1050:1051	arg1	edulis					1053:1058	P. edulis	1050:1058	P. edulis	1050:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	0	5	from	Characterization	0:15	arg1	culm					73:76	Phyllostachys edulis (moso bamboo) culm	38:76	Phyllostachys edulis (moso bamboo) culm during xylogenesis	38:95	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	6	6	from	structure	1017:1025	arg1	xylogenesis					1035:1045	culm xylogenesis	1030:1045	culm xylogenesis in P. edulis	1030:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	3	7	theme	hemicellulosic	531:544	arg1	fractions					546:554	hemicellulosic fractions	531:554	hemicellulosic fractions	531:554	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	5	8	from	xylogenesis	925:935	arg1	edulis					943:948	P. edulis	940:948	P. edulis	940:948	Hemicellulose content and molecular weight increased with culm xylogenesis in P. edulis.					
31227151	4	9	with	glucuronoarabinoxylans	745:766	arg1	arabinose					818:826	side chain arabinose	807:826	side chain arabinose	807:826	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	9	with	glucuronoarabinoxylans	745:766	arg1	acetylation					849:859	acetylation	849:859	acetylation	849:859	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	9	with	glucuronoarabinoxylans	745:766	arg1	backbone					780:787	backbone 1	780:789	backbone 1	780:789	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	9	with	glucuronoarabinoxylans	745:766	arg1	4-β-d-Xyl					792:800	4-β-d-Xyl	792:800	4-β-d-Xyl	792:800	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	9	with	glucuronoarabinoxylans	745:766	arg1	acid					840:843	glucuronic acid	829:843	glucuronic acid	829:843	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	6	10	from	insights	975:982	arg1	dynamics					991:998	the dynamics	987:998	the dynamics of hemicellulose structure in culm xylogenesis in P. edulis	987:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	6	11	from	xylogenesis	1035:1045	arg1	edulis					1053:1058	P. edulis	1050:1058	P. edulis	1050:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	6	11	from	xylogenesis	1035:1045	arg1	dynamics					991:998	the dynamics	987:998	the dynamics of hemicellulose structure in culm xylogenesis in P. edulis	987:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	3	12	theme	fractions	546:554	arg1	composition					484:494	Chemical composition	475:494	Chemical composition	475:494	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	12	theme	fractions	546:554	arg1	characterization					511:526	structural characterization	500:526	structural characterization	500:526	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	1	13	located	found	157:161	arg2	polysaccharides					141:155	β-(1→4)-linked backbone polysaccharides	117:155	β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans	117:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	13	located	found	157:161	arg1	walls					177:181	plant cell walls	166:181	plant cell walls that include xyloglucans, xylans, mannans and glucomannans	166:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	13	located	found	157:161	arg2	Hemicelluloses					98:111	Hemicelluloses	98:111	Hemicelluloses	98:111	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	0	14	theme	edulis	52:57	arg1	culm					73:76	Phyllostachys edulis (moso bamboo) culm	38:76	Phyllostachys edulis (moso bamboo) culm during xylogenesis	38:95	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	6	15	from	dynamics	991:998	arg1	xylogenesis					1035:1045	culm xylogenesis	1030:1045	culm xylogenesis in P. edulis	1030:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	5	16	theme	P.	940:941	arg1	edulis					943:948	P. edulis	940:948	P. edulis	940:948	Hemicellulose content and molecular weight increased with culm xylogenesis in P. edulis.					
31227151	2	17	attach	isolated	334:341	arg1	segments					377:384	the apical, middle and basal segments	348:384	the apical, middle and basal segments of 6 m Phyllostachys edulis culm	348:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	2	17	attach	isolated	334:341	arg2	hemicelluloses					314:327	hemicelluloses	314:327	hemicelluloses	314:327	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	1	18	theme	plant	166:170	arg1	walls					177:181	plant cell walls	166:181	plant cell walls that include xyloglucans, xylans, mannans and glucomannans	166:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	5	19	theme	molecular	888:896	arg1	weight					898:903	molecular weight	888:903	molecular weight	888:903	Hemicellulose content and molecular weight increased with culm xylogenesis in P. edulis.					
31227151	1	20	theme	cell	172:175	arg1	walls					177:181	plant cell walls	166:181	plant cell walls that include xyloglucans, xylans, mannans and glucomannans	166:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	2	21	theme	culm	414:417	arg1	segments					377:384	the apical, middle and basal segments	348:384	the apical, middle and basal segments of 6 m Phyllostachys edulis culm	348:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	1	22	theme	important	252:260	arg1	roles					262:266	important roles	252:266	important roles	252:266	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	4	23	from	chain	697:701	arg1	edulis					726:731	P. edulis	723:731	P. edulis	723:731	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	24	theme	main	692:695	arg1	chain					697:701	the main chain	688:701	the main chain of hemicellulose in P. edulis	688:731	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	25	theme	P.	723:724	arg1	edulis					726:731	P. edulis	723:731	P. edulis	723:731	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	2	26	theme	apical	352:357	arg1	segments					377:384	the apical, middle and basal segments	348:384	the apical, middle and basal segments of 6 m Phyllostachys edulis culm	348:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	2	27	theme	edulis	407:412	arg1	culm					414:417	6 m Phyllostachys edulis culm	389:417	6 m Phyllostachys edulis culm	389:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	6	28	theme	culm	1030:1033	arg1	xylogenesis					1035:1045	culm xylogenesis	1030:1045	culm xylogenesis in P. edulis	1030:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	4	29	theme	hemicellulose	706:718	arg1	chain					697:701	the main chain	688:701	the main chain of hemicellulose in P. edulis	688:731	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	2	30	theme	6 m	389:391	arg1	culm					414:417	6 m Phyllostachys edulis culm	389:417	6 m Phyllostachys edulis culm	389:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	2	31	theme	Phyllostachys	393:405	arg1	culm					414:417	6 m Phyllostachys edulis culm	389:417	6 m Phyllostachys edulis culm	389:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	2	32	theme	extraction	438:447	arg1	procedures					449:458	KOH and DMSO extraction procedures	425:458	KOH and DMSO extraction procedures	425:458	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	1	33	theme	plant	271:275	arg1	configuration					284:296	plant tissue configuration	271:296	plant tissue configuration	271:296	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	34	link	-linked	124:130	arg1	Hemicelluloses					98:111	Hemicelluloses	98:111	Hemicelluloses	98:111	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	34	link	-linked	124:130	arg1	polysaccharides					141:155	β-(1→4)-linked backbone polysaccharides	117:155	β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans	117:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	3	35	theme	13C-	631:634	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	6	36	theme	structure	1017:1025	arg1	dynamics					991:998	the dynamics	987:998	the dynamics of hemicellulose structure in culm xylogenesis in P. edulis	987:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	2	37	theme	DMSO	433:436	arg1	procedures					449:458	KOH and DMSO extraction procedures	425:458	KOH and DMSO extraction procedures	425:458	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	1	38	theme	tissue	277:282	arg1	configuration					284:296	plant tissue configuration	271:296	plant tissue configuration	271:296	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	0	39	theme	Phyllostachys	38:50	arg1	culm					73:76	Phyllostachys edulis (moso bamboo) culm	38:76	Phyllostachys edulis (moso bamboo) culm during xylogenesis	38:95	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	3	40	theme	HPLC	608:611	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	2	41	theme	basal	371:375	arg1	segments					377:384	the apical, middle and basal segments	348:384	the apical, middle and basal segments of 6 m Phyllostachys edulis culm	348:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	0	42	theme	moso	60:63	arg1	culm					73:76	Phyllostachys edulis (moso bamboo) culm	38:76	Phyllostachys edulis (moso bamboo) culm during xylogenesis	38:95	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	3	43	theme	1H-	626:628	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	2	44	theme	KOH	425:427	arg1	procedures					449:458	KOH and DMSO extraction procedures	425:458	KOH and DMSO extraction procedures	425:458	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	4	45	theme	glucuronic	829:838	arg1	backbone					780:787	backbone 1	780:789	backbone 1	780:789	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	4	45	theme	glucuronic	829:838	arg1	acid					840:843	glucuronic acid	829:843	glucuronic acid	829:843	Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation.					
31227151	3	46	theme	NMR	642:644	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	5	47	theme	Hemicellulose	862:874	arg1	content					876:882	Hemicellulose content	862:882	Hemicellulose content	862:882	Hemicellulose content and molecular weight increased with culm xylogenesis in P. edulis.					
31227151	3	48	theme	GPC	614:616	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	49	theme	FT-IR	619:623	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	50	theme	structural	500:509	arg1	characterization					511:526	structural characterization	500:526	structural characterization	500:526	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	51	theme	HSQC	637:640	arg1	NMR					642:644	HSQC NMR	637:644	HSQC NMR	637:644	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	3	52	theme	techniques	654:663	arg1	combination					593:603	a combination	591:603	a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques	591:663	Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, 1H-, 13C-, HSQC NMR and TGA techniques.					
31227151	6	53	theme	hemicellulose	1003:1015	arg1	structure					1017:1025	hemicellulose structure	1003:1025	hemicellulose structure in culm xylogenesis in P. edulis	1003:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	1	54	theme	-linked	124:130	arg1	Hemicelluloses					98:111	Hemicelluloses	98:111	Hemicelluloses	98:111	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	54	theme	-linked	124:130	arg1	polysaccharides					141:155	β-(1→4)-linked backbone polysaccharides	117:155	β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans	117:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	2	55	theme	middle	360:365	arg1	segments					377:384	the apical, middle and basal segments	348:384	the apical, middle and basal segments of 6 m Phyllostachys edulis culm	348:417	In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively.					
31227151	6	56	theme	new	971:973	arg1	insights					975:982	new insights	971:982	new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis	971:1058	Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.					
31227151	0	57	theme	bamboo	65:70	arg1	culm					73:76	Phyllostachys edulis (moso bamboo) culm	38:76	Phyllostachys edulis (moso bamboo) culm during xylogenesis	38:95	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	0	58	theme	hemicelluloses	20:33	arg1	Characterization					0:15	Characterization	0:15	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.	0:96	Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis.					
31227151	1	59	theme	backbone	132:139	arg1	Hemicelluloses					98:111	Hemicelluloses	98:111	Hemicelluloses	98:111	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31227151	1	59	theme	backbone	132:139	arg1	polysaccharides					141:155	β-(1→4)-linked backbone polysaccharides	117:155	β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans	117:240	Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration.					
31173832	3	0	theme	process	303:309	arg1	effects					287:293	the effects	283:293	the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide	283:389	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	4	1	theme	fermented	504:512	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	1	theme	fermented	504:512	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	8	2	theme	Probiotic	931:939	arg1	fermentation					941:952	Probiotic fermentation	931:952	Probiotic fermentation by Bacillus sp	931:967	Probiotic fermentation by Bacillus sp.					
31173832	9	3	theme	immunostimulatory	1035:1051	arg1	activities					1053:1062	immunostimulatory activities	1035:1062	immunostimulatory activities of D. officinale polysaccharide	1035:1094	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	4	4	theme	stem	528:531	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	4	theme	stem	528:531	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	3	5	theme	Dendrobium	224:233	arg1	officinale					235:244	Dendrobium officinale	224:244	Dendrobium officinale (D. officinale) polysaccharides	224:276	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	5	theme	Dendrobium	224:233	arg1	officinale					250:259	D. officinale	247:259	D. officinale	247:259	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	8	6	theme	Bacillus	957:964	arg1	sp					966:967	Bacillus sp	957:967	Bacillus sp	957:967	Probiotic fermentation by Bacillus sp.					
31173832	4	7	theme	D.	476:477	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	7	theme	D.	476:477	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	8	theme	officinale	517:526	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	8	theme	officinale	517:526	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	1	9	theme	Dendrobium	46:55	arg1	polysaccharide					68:81	Dendrobium officinale polysaccharide	46:81	Dendrobium officinale polysaccharide	46:81	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	7	10	theme	nitric	860:865	arg1	oxide					867:871	nitric oxide	860:871	nitric oxide	860:871	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
31173832	7	10	theme	nitric	860:865	arg1	IL-1β					893:897	IL-1β	893:897	IL-1β	893:897	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
31173832	2	11	theme	lactic	150:155	arg1	acid					157:160	a novel lactic acid probiotic	142:170	a novel lactic acid probiotic	142:170	In this study, a novel lactic acid probiotic named Bacillus sp.					
31173832	1	12	theme	officinale	57:66	arg1	polysaccharide					68:81	Dendrobium officinale polysaccharide	46:81	Dendrobium officinale polysaccharide	46:81	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	1	13	from	fermentation	30:41	arg1	polysaccharide					68:81	Dendrobium officinale polysaccharide	46:81	Dendrobium officinale polysaccharide	46:81	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	9	14	theme	D.	1067:1068	arg1	polysaccharide					1081:1094	D. officinale polysaccharide	1067:1094	D. officinale polysaccharide	1067:1094	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	2	15	theme	novel	144:148	arg1	acid					157:160	a novel lactic acid probiotic	142:170	a novel lactic acid probiotic	142:170	In this study, a novel lactic acid probiotic named Bacillus sp.					
31173832	3	16	theme	D.	247:248	arg1	officinale					235:244	Dendrobium officinale	224:244	Dendrobium officinale (D. officinale) polysaccharides	224:276	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	16	theme	D.	247:248	arg1	officinale					250:259	D. officinale	247:259	D. officinale	247:259	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	9	17	theme	officinale	1070:1079	arg1	polysaccharide					1081:1094	D. officinale polysaccharide	1067:1094	D. officinale polysaccharide	1067:1094	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	7	18	theme	cell	837:840	arg1	proliferation					842:854	cell proliferation	837:854	cell proliferation	837:854	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
31173832	3	19	theme	immunostimulatory	332:348	arg1	activity					350:357	immunostimulatory activity	332:357	immunostimulatory activity	332:357	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	5	20	theme	average	614:620	arg1	Mw					640:641	Mw	640:641	Mw	640:641	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	5	20	theme	average	614:620	arg1	weight					632:637	the average molecular weight	610:637	the average molecular weight (Mw) of FDP-1	610:651	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	7	21	theme	RAW	914:916	arg1	cells					924:928	RAW 264.7 cells	914:928	RAW 264.7 cells	914:928	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
31173832	10	22	theme	functional	1207:1216	arg1	material					1218:1225	auxiliary functional material	1197:1225	auxiliary functional material in immunotherapy	1197:1242	DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.					
31173832	9	23	theme	polysaccharide	1081:1094	arg1	activities					1053:1062	immunostimulatory activities	1035:1062	immunostimulatory activities of D. officinale polysaccharide	1035:1094	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	3	24	theme	polysaccharide	376:389	arg1	structure					318:326	structure	318:326	structure	318:326	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	24	theme	polysaccharide	376:389	arg1	activity					350:357	immunostimulatory activity	332:357	immunostimulatory activity	332:357	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	25	theme	officinale	235:244	arg1	polysaccharides					262:276	Dendrobium officinale (D. officinale) polysaccharides	224:276	Dendrobium officinale (D. officinale) polysaccharides	224:276	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	26	theme	D.	362:363	arg1	polysaccharide					376:389	D. officinale polysaccharide	362:389	D. officinale polysaccharide	362:389	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	1	27	theme	immunoregulatory	98:113	arg1	activities					115:124	immunoregulatory activities	98:124	immunoregulatory activities	98:124	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	1	28	dep	fermentation	30:41	arg1	Structure					84:92	Structure	84:92	Structure	84:92	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	1	28	dep	fermentation	30:41	arg1	activities					115:124	immunoregulatory activities	98:124	immunoregulatory activities	98:124	DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.					
31173832	3	29	theme	officinale	365:374	arg1	polysaccharide					376:389	D. officinale polysaccharide	362:389	D. officinale polysaccharide	362:389	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	2	30	theme	Bacillus	178:185	arg1	sp					187:188	Bacillus sp	178:188	Bacillus sp	178:188	In this study, a novel lactic acid probiotic named Bacillus sp.					
31173832	9	31	theme	possible	1110:1117	arg1	application					1119:1129	the possible application	1106:1129	the possible application of Bacillus sp	1106:1144	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	4	32	from	UDP-1	496:500	arg1	FLP-1					582:586	FLP-1	582:586	FLP-1	582:586	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	32	from	UDP-1	496:500	arg1	liquid					574:579	liquid	574:579	liquid	574:579	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	10	33	from	material	1218:1225	arg1	immunotherapy					1230:1242	immunotherapy	1230:1242	immunotherapy	1230:1242	DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.					
31173832	4	34	theme	unfermented	464:474	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	34	theme	unfermented	464:474	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	3	35	dep	structure	318:326	arg1	the					314:316	the	314:316	the	314:316	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	2	36	theme	probiotic	162:170	arg1	acid					157:160	a novel lactic acid probiotic	142:170	a novel lactic acid probiotic	142:170	In this study, a novel lactic acid probiotic named Bacillus sp.					
31173832	0	37	theme	sp	19:20	arg1	Effect					0:5	Effect	0:5	Effect of Bacillus sp.	0:21	Effect of Bacillus sp.					
31173832	6	38	theme	mannose	750:756	arg1	proportions					735:745	the proportions	731:745	the proportions of mannose in FDP-1	731:765	Fermentation increased the proportions of mannose in FDP-1 by 51.38% compared with that in UDP-1.					
31173832	4	39	from	polysaccharide	546:559	arg1	FLP-1					582:586	FLP-1	582:586	FLP-1	582:586	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	39	from	polysaccharide	546:559	arg1	liquid					574:579	liquid	574:579	liquid	574:579	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	10	40	theme	officinale	1167:1176	arg1	polysaccharides					1178:1192	D. officinale polysaccharides	1164:1192	D. officinale polysaccharides	1164:1192	DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.					
31173832	5	41	theme	FDP-1	647:651	arg1	Mw					640:641	Mw	640:641	Mw	640:641	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	5	41	theme	FDP-1	647:651	arg1	weight					632:637	the average molecular weight	610:637	the average molecular weight (Mw) of FDP-1	610:651	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	4	42	theme	officinale	479:488	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	42	theme	officinale	479:488	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	9	43	theme	Bacillus	1134:1141	arg1	sp					1143:1144	Bacillus sp	1134:1144	Bacillus sp	1134:1144	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	6	44	from	proportions	735:745	arg1	FDP-1					761:765	FDP-1	761:765	FDP-1	761:765	Fermentation increased the proportions of mannose in FDP-1 by 51.38% compared with that in UDP-1.					
31173832	9	45	theme	monosaccharide	989:1002	arg1	composition					1004:1014	monosaccharide composition	989:1014	monosaccharide composition	989:1014	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	10	46	theme	D.	1164:1165	arg1	polysaccharides					1178:1192	D. officinale polysaccharides	1164:1192	D. officinale polysaccharides	1164:1192	DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.					
31173832	3	47	theme	such	298:301	arg1	process					303:309	such process	298:309	such process	298:309	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	9	48	theme	sp	1143:1144	arg1	application					1119:1129	the possible application	1106:1129	the possible application of Bacillus sp	1106:1144	DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp.					
31173832	4	49	theme	stem	490:493	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	49	theme	stem	490:493	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	5	50	theme	molecular	622:630	arg1	Mw					640:641	Mw	640:641	Mw	640:641	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	5	50	theme	molecular	622:630	arg1	weight					632:637	the average molecular weight	610:637	the average molecular weight (Mw) of FDP-1	610:651	After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92 × 105 Da (UDP-1) to 5.21 × 105 Da.					
31173832	7	51	theme	interleukin-1β	877:890	arg1	production					900:909	nitric oxide and interleukin-1β (IL-1β) production	860:909	nitric oxide and interleukin-1β (IL-1β) production	860:909	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
31173832	3	52	from	effects	287:293	arg1	structure					318:326	structure	318:326	structure	318:326	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	3	52	from	effects	287:293	arg1	activity					350:357	immunostimulatory activity	332:357	immunostimulatory activity	332:357	DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated.					
31173832	10	53	theme	auxiliary	1197:1205	arg1	material					1218:1225	auxiliary functional material	1197:1225	auxiliary functional material in immunotherapy	1197:1242	DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.					
31173832	4	54	theme	D.	514:515	arg1	FDP-1					534:538	fermented D. officinale stem (FDP-1)	504:539	fermented D. officinale stem (FDP-1)	504:539	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	4	54	theme	D.	514:515	arg1	UDP-1					496:500	unfermented D. officinale stem (UDP-1)	464:501	unfermented D. officinale stem (UDP-1)	464:501	Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1).					
31173832	0	55	theme	Bacillus	10:17	arg1	sp					19:20	Bacillus sp	10:20	Bacillus sp	10:20	Effect of Bacillus sp.					
31173832	7	56	theme	oxide	867:871	arg1	production					900:909	nitric oxide and interleukin-1β (IL-1β) production	860:909	nitric oxide and interleukin-1β (IL-1β) production	860:909	FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1β (IL-1β) production in RAW 264.7 cells.					
30864128	0	0	theme	Polysaccharides	75:89	arg1	O-Acetylation					38:50	the O-Acetylation	34:50	the O-Acetylation of Bacterial Cell Wall Polysaccharides	34:89	Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides.					
30864128	4	1	theme	inhibitors	549:558	arg1	search					539:544	search	539:544	search of inhibitors of the respective enzymes	539:584	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	0	2	theme	Wall	70:73	arg1	Polysaccharides					75:89	Bacterial Cell Wall Polysaccharides	55:89	Bacterial Cell Wall Polysaccharides	55:89	Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides.					
30864128	4	3	theme	respective	567:576	arg1	enzymes					578:584	the respective enzymes	563:584	the respective enzymes	563:584	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	3	4	theme	wall	375:378	arg1	O-acetylation					380:392	bacterial cell wall O-acetylation	360:392	bacterial cell wall O-acetylation	360:392	Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases.					
30864128	4	5	theme	screens	528:534	arg1	development					497:507	the development	493:507	the development of high-throughput screens in search of inhibitors of the respective enzymes	493:584	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	3	6	theme	O-acetylation	380:392	arg1	analysis					348:355	the compositional analysis	330:355	the compositional analysis of bacterial cell wall O-acetylation	330:392	Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases.					
30864128	0	7	theme	Cell	65:68	arg1	Polysaccharides					75:89	Bacterial Cell Wall Polysaccharides	55:89	Bacterial Cell Wall Polysaccharides	55:89	Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides.					
30864128	4	8	from	development	497:507	arg1	search					539:544	search	539:544	search of inhibitors of the respective enzymes	539:584	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	4	9	theme	high-throughput	512:526	arg1	screens					528:534	high-throughput screens	512:534	high-throughput screens	512:534	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	4	10	theme	enzymes	578:584	arg1	inhibitors					549:558	inhibitors	549:558	inhibitors of the respective enzymes	549:584	The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.					
30864128	0	11	theme	Bacterial	55:63	arg1	Polysaccharides					75:89	Bacterial Cell Wall Polysaccharides	55:89	Bacterial Cell Wall Polysaccharides	55:89	Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides.					
30864128	2	12	theme	systems	242:248	arg1	hydrolases					214:223	hydrolases	214:223	hydrolases of innate immune systems	214:248	This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity.					
30864128	3	13	theme	compositional	334:346	arg1	analysis					348:355	the compositional analysis	330:355	the compositional analysis of bacterial cell wall O-acetylation	330:392	Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases.					
30864128	2	14	theme	innate	228:233	arg1	systems					242:248	innate immune systems	228:248	innate immune systems	228:248	This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity.					
30864128	2	15	theme	immune	235:240	arg1	systems					242:248	innate immune systems	228:248	innate immune systems	228:248	This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity.					
30864128	1	16	theme	bacterial	126:134	arg1	walls					141:145	bacterial cell walls	126:145	bacterial cell walls	126:145	The polysaccharides that comprise bacterial cell walls are commonly O-acetylated.					
30864128	1	17	theme	cell	136:139	arg1	walls					141:145	bacterial cell walls	126:145	bacterial cell walls	126:145	The polysaccharides that comprise bacterial cell walls are commonly O-acetylated.					
30864128	3	18	theme	bacterial	360:368	arg1	O-acetylation					380:392	bacterial cell wall O-acetylation	360:392	bacterial cell wall O-acetylation	360:392	Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases.					
30864128	2	19	theme	autolytic	277:285	arg1	activity					287:294	endogenous autolytic activity	266:294	endogenous autolytic activity	266:294	This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity.					
30864128	3	20	theme	cell	370:373	arg1	O-acetylation					380:392	bacterial cell wall O-acetylation	360:392	bacterial cell wall O-acetylation	360:392	Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases.					
30864128	2	21	theme	endogenous	266:275	arg1	activity					287:294	endogenous autolytic activity	266:294	endogenous autolytic activity	266:294	This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity.					
29930401	0	0	theme	carbohydrate	61:72	arg1	source					74:79	main carbohydrate source	56:79	main carbohydrate source	56:79	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	3	1	from	effects	376:382	arg1	performance					536:546	cognitive performance	526:546	cognitive performance of piglets	526:557	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	0	2	theme	main	56:59	arg1	source					74:79	main carbohydrate source	56:79	main carbohydrate source	56:79	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	7	3	contain	have	1174:1177	arg2	scores					1189:1194	higher RM scores	1179:1194	higher RM scores	1179:1194	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	7	3	contain	have	1174:1177	arg1	piglets					1117:1123	piglets	1117:1123	piglets offered the maltodextrin-based formula	1117:1162	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	4	4	theme	buckets	783:789	arg1	configuration					759:771	a configuration	757:771	a configuration of baited buckets	757:789	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	8	5	theme	Working	1269:1275	arg1	memory					1290:1295	Working (short-term) memory	1269:1295	Working (short-term) memory	1269:1295	Working (short-term) memory was not affected by the formulas.					
29930401	9	6	dep	weeks	1444:1448	arg1	end					1460:1462	the end	1456:1462	the end of the intervention	1456:1482	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	5	7	theme	reversal	850:857	arg1	trials					859:864	16 reversal trials	847:864	16 reversal trials in which the location of the baited buckets was changed	847:920	After 28 acquisition trials, piglets were subjected to 16 reversal trials in which the location of the baited buckets was changed.					
29930401	7	8	theme	configuration	1102:1114	arg1	switch					1092:1097	the switch	1088:1097	the switch of configuration	1088:1114	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	7	9	theme	RM	1211:1212	arg1	errors					1214:1219	fewer RM errors	1205:1219	fewer RM errors	1205:1219	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	4	10	theme	holeboard	680:688	arg1	task					690:693	a spatial holeboard task	670:693	a spatial holeboard task (n = 8 pens/formula)	670:714	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	4	10	theme	holeboard	680:688	arg1	pens/formula					702:713	n = 8 pens/formula	696:713	n = 8 pens/formula	696:713	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	2	11	theme	different	254:262	arg1	carbohydrates					264:276	different carbohydrates	254:276	different carbohydrates than lactose	254:289	The impact of using different carbohydrates than lactose on later cognition of formula-fed infants remains, however, unknown.					
29930401	7	12	theme	RM	1186:1187	arg1	scores					1189:1194	higher RM scores	1179:1194	higher RM scores	1179:1194	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	0	13	theme	cognitive	91:99	arg1	performance					101:111	cognitive performance	91:111	cognitive performance of piglets	91:122	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	9	14	theme	milk	1379:1382	arg1	formulas					1384:1391	milk formulas	1379:1391	milk formulas	1379:1391	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	3	15	theme	nutrient	501:508	arg1	composition					510:520	total nutrient composition	495:520	total nutrient composition	495:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	6	16	theme	memory	987:992	arg1	scores					999:1004	higher reference memory (RM) scores	970:1004	higher reference memory (RM) scores	970:1004	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	6	17	contain	had	966:968	arg1	Piglets					923:929	Piglets	923:929	Piglets fed the maltodextrin-based formula	923:964	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	6	17	contain	had	966:968	arg2	scores					999:1004	higher reference memory (RM) scores	970:1004	higher reference memory (RM) scores	970:1004	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	3	18	theme	composition	510:520	arg1	%					490:490	28%	488:490	28% of total nutrient composition	488:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	3	18	theme	composition	510:520	arg1	composition					510:520	total nutrient composition	495:520	total nutrient composition	495:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	1	19	theme	recent	146:151	arg1	years					153:157	recent years	146:157	recent years	146:157	In recent years, lactose-free and low-lactose infant formulas have been increasingly used.					
29930401	0	20	theme	milk	2:5	arg1	formula					7:13	A milk formula	0:13	A milk formula containing maltodextrin, vs. lactose	0:50	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	4	21	theme	baited	776:781	arg1	buckets					783:789	baited buckets	776:789	baited buckets	776:789	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	0	22	contain	containing	15:24	arg1	formula					7:13	A milk formula	0:13	A milk formula containing maltodextrin, vs. lactose	0:50	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	0	22	contain	containing	15:24	arg2	lactose					44:50	lactose	44:50	lactose	44:50	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	0	22	contain	containing	15:24	arg2	maltodextrin					26:37	maltodextrin	26:37	maltodextrin	26:37	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	2	23	theme	later	294:298	arg1	cognition					300:308	later cognition	294:308	later cognition of formula-fed infants	294:331	The impact of using different carbohydrates than lactose on later cognition of formula-fed infants remains, however, unknown.					
29930401	3	24	theme	formulas	397:404	arg1	effects					376:382	the effects	372:382	the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets	372:557	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	3	25	theme	piglets	551:557	arg1	performance					536:546	cognitive performance	526:546	cognitive performance of piglets	526:557	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	4	26	dep	received	568:575	arg1	tested					660:665	tested	660:665	were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets	642:789	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	3	27	theme	cognitive	526:534	arg1	performance					536:546	cognitive performance	526:546	cognitive performance of piglets	526:557	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	1	28	theme	lactose-free	160:171	arg1	formulas					196:203	lactose-free and low-lactose infant formulas	160:203	lactose-free and low-lactose infant formulas	160:203	In recent years, lactose-free and low-lactose infant formulas have been increasingly used.					
29930401	4	29	theme	spatial	672:678	arg1	task					690:693	a spatial holeboard task	670:693	a spatial holeboard task (n = 8 pens/formula)	670:714	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	4	29	theme	spatial	672:678	arg1	pens/formula					702:713	n = 8 pens/formula	696:713	n = 8 pens/formula	696:713	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	3	30	dep	source	480:485	arg1	%					490:490	28%	488:490	28% of total nutrient composition	488:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	3	30	dep	source	480:485	arg1	composition					510:520	total nutrient composition	495:520	total nutrient composition	495:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	8	31	theme	short-term	1278:1287	arg1	memory					1290:1295	Working (short-term) memory	1269:1295	Working (short-term) memory	1269:1295	Working (short-term) memory was not affected by the formulas.					
29930401	2	32	from	impact	238:243	arg1	cognition					300:308	later cognition	294:308	later cognition of formula-fed infants	294:331	The impact of using different carbohydrates than lactose on later cognition of formula-fed infants remains, however, unknown.					
29930401	9	33	theme	spatial	1412:1418	arg1	memory					1420:1425	long-term spatial memory	1402:1425	long-term spatial memory of piglets	1402:1436	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	7	34	theme	fewer	1205:1209	arg1	errors					1214:1219	fewer RM errors	1205:1219	fewer RM errors	1205:1219	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	6	35	theme	lactose-based	1027:1039	arg1	formula					1041:1047	the lactose-based formula	1023:1047	the lactose-based formula towards the end of acquisition	1023:1078	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	4	36	theme	age	611:613	arg1	weeks					602:606	1 to 9 weeks	595:606	1 to 9 weeks of age	595:613	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	9	37	theme	intervention	1471:1482	arg1	end					1460:1462	the end	1456:1462	the end of the intervention	1456:1482	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	6	38	theme	reference	977:985	arg1	memory					987:992	reference memory	977:992	higher reference memory (RM) scores	970:1004	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	6	38	theme	reference	977:985	arg1	RM					995:996	RM	995:996	RM	995:996	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	1	39	theme	low-lactose	177:187	arg1	formulas					196:203	lactose-free and low-lactose infant formulas	160:203	lactose-free and low-lactose infant formulas	160:203	In recent years, lactose-free and low-lactose infant formulas have been increasingly used.					
29930401	3	40	theme	providing	387:395	arg1	formulas					397:404	providing formulas	387:404	providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition)	387:521	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	0	41	theme	piglets	116:122	arg1	performance					101:111	cognitive performance	91:111	cognitive performance of piglets	91:122	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	1	42	theme	infant	189:194	arg1	formulas					196:203	lactose-free and low-lactose infant formulas	160:203	lactose-free and low-lactose infant formulas	160:203	In recent years, lactose-free and low-lactose infant formulas have been increasingly used.					
29930401	0	43	theme	spatial	129:135	arg1	task					137:140	a spatial task	127:140	a spatial task	127:140	A milk formula containing maltodextrin, vs. lactose, as main carbohydrate source, improves cognitive performance of piglets in a spatial task.					
29930401	1	44	used	used	228:231	arg2	formulas					196:203	lactose-free and low-lactose infant formulas	160:203	lactose-free and low-lactose infant formulas	160:203	In recent years, lactose-free and low-lactose infant formulas have been increasingly used.					
29930401	9	45	theme	maltodextrin	1363:1374	arg1	use					1356:1358	the use	1352:1358	the use of maltodextrin in milk formulas	1352:1391	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	2	46	theme	infants	325:331	arg1	cognition					300:308	later cognition	294:308	later cognition of formula-fed infants	294:331	The impact of using different carbohydrates than lactose on later cognition of formula-fed infants remains, however, unknown.					
29930401	9	47	theme	long-term	1402:1410	arg1	memory					1420:1425	long-term spatial memory	1402:1425	long-term spatial memory of piglets	1402:1436	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	9	48	theme	piglets	1430:1436	arg1	memory					1420:1425	long-term spatial memory	1402:1425	long-term spatial memory of piglets	1402:1436	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	3	49	contain	containing	406:415	arg1	formulas					397:404	providing formulas	387:404	providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition)	387:521	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	3	49	contain	containing	406:415	arg2	lactose					451:457	lactose	451:457	lactose	451:457	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	3	49	contain	containing	406:415	arg2	maltodextrin					435:446	digestible maltodextrin	424:446	digestible maltodextrin	424:446	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	4	50	dep	9	600:600	arg1	to					597:598	to	597:598	to	597:598	Piglets received the formulas from 1 to 9 weeks of age and, starting at 12 weeks, were individually tested in a spatial holeboard task (n = 8 pens/formula), in which they had to learn and memorize a configuration of baited buckets.					
29930401	9	51	from	use	1356:1358	arg1	formulas					1384:1391	milk formulas	1379:1391	milk formulas	1379:1391	Compared to lactose, the use of maltodextrin in milk formulas improved long-term spatial memory of piglets, even weeks after the end of the intervention.					
29930401	2	52	theme	formula-fed	313:323	arg1	infants					325:331	formula-fed infants	313:331	formula-fed infants	313:331	The impact of using different carbohydrates than lactose on later cognition of formula-fed infants remains, however, unknown.					
29930401	5	53	theme	baited	895:900	arg1	buckets					902:908	the baited buckets	891:908	the baited buckets	891:908	After 28 acquisition trials, piglets were subjected to 16 reversal trials in which the location of the baited buckets was changed.					
29930401	3	54	theme	main	462:465	arg1	source					480:485	main carbohydrate source	462:485	main carbohydrate source (28% of total nutrient composition)	462:521	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	6	55	theme	acquisition	1068:1078	arg1	end					1061:1063	the end	1057:1063	the end of acquisition	1057:1078	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	5	56	theme	buckets	902:908	arg1	location					879:886	the location	875:886	the location of the baited buckets	875:908	After 28 acquisition trials, piglets were subjected to 16 reversal trials in which the location of the baited buckets was changed.					
29930401	3	57	theme	carbohydrate	467:478	arg1	source					480:485	main carbohydrate source	462:485	main carbohydrate source (28% of total nutrient composition)	462:521	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	7	58	theme	higher	1179:1184	arg1	scores					1189:1194	higher RM scores	1179:1194	higher RM scores	1179:1194	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	6	59	theme	maltodextrin-based	939:956	arg1	formula					958:964	the maltodextrin-based formula	935:964	the maltodextrin-based formula	935:964	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	7	60	theme	lactose-based	1246:1258	arg1	formula					1260:1266	the lactose-based formula	1242:1266	the lactose-based formula	1242:1266	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	6	61	theme	higher	970:975	arg1	scores					999:1004	higher reference memory (RM) scores	970:1004	higher reference memory (RM) scores	970:1004	Piglets fed the maltodextrin-based formula had higher reference memory (RM) scores than piglets fed the lactose-based formula towards the end of acquisition.					
29930401	5	62	theme	acquisition	801:811	arg1	trials					813:818	28 acquisition trials	798:818	28 acquisition trials	798:818	After 28 acquisition trials, piglets were subjected to 16 reversal trials in which the location of the baited buckets was changed.					
29930401	3	63	theme	digestible	424:433	arg1	maltodextrin					435:446	digestible maltodextrin	424:446	digestible maltodextrin	424:446	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
29930401	7	64	theme	maltodextrin-based	1137:1154	arg1	formula					1156:1162	the maltodextrin-based formula	1133:1162	the maltodextrin-based formula	1133:1162	During the switch of configuration, piglets offered the maltodextrin-based formula tended to have higher RM scores and make fewer RM errors than piglets offered the lactose-based formula.					
29930401	3	65	theme	total	495:499	arg1	composition					510:520	total nutrient composition	495:520	total nutrient composition	495:520	We examined the effects of providing formulas containing either digestible maltodextrin or lactose as main carbohydrate source (28% of total nutrient composition) on cognitive performance of piglets.					
31753363	0	0	theme	hydrogels	84:92	arg1	capacity					72:79	the scaffolding capacity	56:79	the scaffolding capacity of hydrogels	56:92	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	2	1	theme	X-ray	527:531	arg1	scattering					533:542	wide-angle X-ray scattering	516:542	wide-angle X-ray scattering	516:542	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	4	2	theme	pore	752:755	arg1	networks					757:764	interconnecting pore networks	736:764	interconnecting pore networks	736:764	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	2	3	theme	wide-angle	516:525	arg1	scattering					533:542	wide-angle X-ray scattering	516:542	wide-angle X-ray scattering	516:542	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	6	4	theme	PBS	1116:1118	arg1	pH					1128:1129	pH 7.4	1128:1133	pH 7.4	1128:1133	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	6	4	theme	PBS	1116:1118	arg1	buffer					1120:1125	PBS buffer	1116:1125	PBS buffer (pH 7.4)	1116:1134	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	3	5	dep	excesses	614:621	arg1	ranging					624:630	ranging	624:630	ranging from 60 to 80 wt%	624:648	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%) were created.					
31753363	8	6	theme	CS/GG	1337:1341	arg1	scaffolds					1371:1379	scaffolds	1371:1379	scaffolds for tissue engineering purposes	1371:1411	This work presents for the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes.					
31753363	8	6	theme	CS/GG	1337:1341	arg1	hydrogels					1343:1351	CS/GG hydrogels	1337:1351	CS/GG hydrogels	1337:1351	This work presents for the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes.					
31753363	0	7	theme	bone	97:100	arg1	cells					119:123	bone mesenchymal stem cells	97:123	bone mesenchymal stem cells	97:123	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	1	8	theme	non-soluble	344:354	arg1	materials					377:385	non-soluble polysaccharide-based materials	344:385	non-soluble polysaccharide-based materials	344:385	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	5	9	theme	cells	994:998	arg1	growth					940:945	growth	940:945	growth	940:945	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	9	theme	cells	994:998	arg1	spreading					952:960	spreading	952:960	spreading	952:960	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	9	theme	cells	994:998	arg1	attachment					928:937	attachment	928:937	attachment	928:937	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	2	10	theme	electron	558:565	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy	549:576	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	1	11	theme	polysaccharide-based	356:375	arg1	materials					377:385	non-soluble polysaccharide-based materials	344:385	non-soluble polysaccharide-based materials	344:385	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	3	12	theme	chitosan	600:607	arg1	excesses					614:621	chitosan (CS) excesses	600:621	chitosan (CS) excesses (ranging from 60 to 80 wt%)	600:649	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%) were created.					
31753363	2	13	theme	scanning	549:556	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy	549:576	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	3	14	dep	80 wt	643:647	arg1	to					640:641	to	640:641	to	640:641	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%) were created.					
31753363	8	15	theme	first	1321:1325	arg1	time					1327:1330	the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes	1317:1411	the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes	1317:1411	This work presents for the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes.					
31753363	2	16	theme	physical	419:426	arg1	hydrogels					428:436	physical hydrogels	419:436	physical hydrogels called as PECs	419:451	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	0	17	theme	mesenchymal	102:112	arg1	cells					119:123	bone mesenchymal stem cells	97:123	bone mesenchymal stem cells	97:123	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	5	18	theme	weight	861:866	arg1	ratio					868:872	80/20 CS/GG weight ratio	849:872	80/20 CS/GG weight ratio (sample CS/GG80-20)	849:892	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	18	theme	weight	861:866	arg1	CS/GG80-20					882:891	sample CS/GG80-20	875:891	sample CS/GG80-20	875:891	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	6	19	theme	PEC	1109:1111	arg1	capacity					1093:1100	the swelling capacity	1080:1100	the swelling capacity of the PEC in PBS buffer (pH 7.4)	1080:1134	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	0	20	theme	stem	114:117	arg1	cells					119:123	bone mesenchymal stem cells	97:123	bone mesenchymal stem cells	97:123	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	0	21	theme	gum	16:18	arg1	content					26:32	Chitosan/gellan gum ratio content	0:32	Chitosan/gellan gum ratio content into blends	0:44	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	5	22	theme	CS/GG	822:826	arg1	hydrogel					828:835	The CS/GG hydrogel	818:835	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20)	818:892	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	22	theme	CS/GG	822:826	arg1	cytocompatible					897:910	cytocompatible	897:910	cytocompatible	897:910	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	23	theme	marrow	970:975	arg1	BMSCs					1001:1005	BMSCs	1001:1005	BMSCs	1001:1005	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	23	theme	marrow	970:975	arg1	cells					994:998	bone marrow mesenchymal stem cells	965:998	bone marrow mesenchymal stem cells (BMSCs)	965:1006	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	4	24	theme	structural	709:718	arg1	homogeneity					720:730	structural homogeneity	709:730	structural homogeneity	709:730	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	0	25	theme	Chitosan/gellan	0:14	arg1	content					26:32	Chitosan/gellan gum ratio content	0:32	Chitosan/gellan gum ratio content into blends	0:44	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	5	26	theme	mesenchymal	977:987	arg1	BMSCs					1001:1005	BMSCs	1001:1005	BMSCs	1001:1005	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	26	theme	mesenchymal	977:987	arg1	cells					994:998	bone marrow mesenchymal stem cells	965:998	bone marrow mesenchymal stem cells (BMSCs)	965:1006	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	2	27	theme	polyelectrolyte	392:406	arg1	complexes					408:416	The polyelectrolyte complexes	388:416	The polyelectrolyte complexes (physical hydrogels called as PECs)	388:452	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	7	28	theme	CS	1156:1157	arg1	content					1159:1165	the CS content	1152:1165	the CS content	1152:1165	By controlling the CS content, we can tune the water uptake of the material, enhancing the capacity to serve as a three-dimensional cell scaffold for BMSCs.					
31753363	1	29	theme	covalent	272:279	arg1	agents					307:312	any covalent and metallic crosslinking agents	268:312	any covalent and metallic crosslinking agents	268:312	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	4	30	theme	weight	804:809	arg1	ratio					811:815	the CS/GG weight ratio	794:815	the CS/GG weight ratio	794:815	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	6	31	from	capacity	1093:1100	arg1	pH					1128:1129	pH 7.4	1128:1133	pH 7.4	1128:1133	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	6	31	from	capacity	1093:1100	arg1	buffer					1120:1125	PBS buffer	1116:1125	PBS buffer (pH 7.4)	1116:1134	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	1	32	theme	hydrogel	192:199	arg1	scaffolds					201:209	hydrogel scaffolds	192:209	hydrogel scaffolds	192:209	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	0	33	theme	ratio	20:24	arg1	content					26:32	Chitosan/gellan gum ratio content	0:32	Chitosan/gellan gum ratio content into blends	0:44	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	5	34	theme	stem	989:992	arg1	BMSCs					1001:1005	BMSCs	1001:1005	BMSCs	1001:1005	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	34	theme	stem	989:992	arg1	cells					994:998	bone marrow mesenchymal stem cells	965:998	bone marrow mesenchymal stem cells (BMSCs)	965:1006	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	4	35	theme	CS/GG	798:802	arg1	ratio					811:815	the CS/GG weight ratio	794:815	the CS/GG weight ratio	794:815	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	1	36	theme	scaffolds	201:209	arg1	characterization					172:187	characterization	172:187	characterization	172:187	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	1	36	theme	scaffolds	201:209	arg1	production					157:166	production	157:166	production	157:166	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	1	37	theme	metallic	285:292	arg1	agents					307:312	any covalent and metallic crosslinking agents	268:312	any covalent and metallic crosslinking agents	268:312	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	7	38	theme	three-dimensional	1251:1267	arg1	scaffold					1274:1281	a three-dimensional cell scaffold	1249:1281	a three-dimensional cell scaffold for BMSCs	1249:1291	By controlling the CS content, we can tune the water uptake of the material, enhancing the capacity to serve as a three-dimensional cell scaffold for BMSCs.					
31753363	5	39	theme	culture	1032:1038	arg1	days					1019:1022	nine days	1014:1022	nine days of cell culture	1014:1038	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	6	40	theme	swelling	1084:1091	arg1	capacity					1093:1100	the swelling capacity	1080:1100	the swelling capacity of the PEC in PBS buffer (pH 7.4)	1080:1134	The cytocompatibility is correlated to the swelling capacity of the PEC in PBS buffer (pH 7.4).					
31753363	4	41	theme	Durable	665:671	arg1	scaffolds					694:702	Durable polysaccharide-based scaffolds	665:702	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks	665:764	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	1	42	theme	crosslinking	294:305	arg1	agents					307:312	any covalent and metallic crosslinking agents	268:312	any covalent and metallic crosslinking agents	268:312	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	4	43	with	scaffolds	694:702	arg1	homogeneity					720:730	structural homogeneity	709:730	structural homogeneity	709:730	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	4	43	with	scaffolds	694:702	arg1	networks					757:764	interconnecting pore networks	736:764	interconnecting pore networks	736:764	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	7	44	theme	cell	1269:1272	arg1	scaffold					1274:1281	a three-dimensional cell scaffold	1249:1281	a three-dimensional cell scaffold for BMSCs	1249:1291	By controlling the CS content, we can tune the water uptake of the material, enhancing the capacity to serve as a three-dimensional cell scaffold for BMSCs.					
31753363	2	45	theme	infrared	493:500	arg1	spectroscopy					502:513	Fourier-transform infrared spectroscopy	475:513	Fourier-transform infrared spectroscopy	475:513	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	5	46	theme	80/20	849:853	arg1	ratio					868:872	80/20 CS/GG weight ratio	849:872	80/20 CS/GG weight ratio (sample CS/GG80-20)	849:892	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	46	theme	80/20	849:853	arg1	CS/GG80-20					882:891	sample CS/GG80-20	875:891	sample CS/GG80-20	875:891	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	7	47	theme	material	1204:1211	arg1	uptake					1190:1195	the water uptake	1180:1195	the water uptake of the material	1180:1211	By controlling the CS content, we can tune the water uptake of the material, enhancing the capacity to serve as a three-dimensional cell scaffold for BMSCs.					
31753363	1	48	dep	production	157:166	arg1	the					153:155	the	153:155	the	153:155	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	2	49	theme	Fourier-transform	475:491	arg1	spectroscopy					502:513	Fourier-transform infrared spectroscopy	475:513	Fourier-transform infrared spectroscopy	475:513	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	8	50	theme	tissue	1385:1390	arg1	purposes					1404:1411	tissue engineering purposes	1385:1411	tissue engineering purposes	1385:1411	This work presents for the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes.					
31753363	3	51	contain	containing	589:598	arg2	excesses					614:621	chitosan (CS) excesses	600:621	chitosan (CS) excesses (ranging from 60 to 80 wt%)	600:649	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%) were created.					
31753363	3	51	contain	containing	589:598	arg1	Hydrogels					579:587	Hydrogels	579:587	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%)	579:649	Hydrogels containing chitosan (CS) excesses (ranging from 60 to 80 wt%) were created.					
31753363	2	52	dep	complexes	408:416	arg1	hydrogels					428:436	physical hydrogels	419:436	physical hydrogels called as PECs	419:451	The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and scanning electron microscopy.					
31753363	4	53	theme	interconnecting	736:750	arg1	networks					757:764	interconnecting pore networks	736:764	interconnecting pore networks	736:764	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	1	54	theme	chitosan/gellan	220:234	arg1	CS/GG					241:245	CS/GG	241:245	CS/GG	241:245	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	1	54	theme	chitosan/gellan	220:234	arg1	gum					236:238	chitosan/gellan gum	220:238	chitosan/gellan gum (CS/GG) assemblies	220:257	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	5	55	theme	bone	965:968	arg1	marrow					970:975	bone marrow	965:975	bone marrow mesenchymal stem cells (BMSCs)	965:1006	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	1	56	theme	gum	236:238	arg1	assemblies					248:257	chitosan/gellan gum (CS/GG) assemblies	220:257	chitosan/gellan gum (CS/GG) assemblies	220:257	Here, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials.					
31753363	5	57	dep	attachment	928:937	arg1	the					924:926	the	924:926	the	924:926	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	8	58	theme	engineering	1392:1402	arg1	purposes					1404:1411	tissue engineering purposes	1385:1411	tissue engineering purposes	1385:1411	This work presents for the first time that CS/GG hydrogels can be applied as scaffolds for tissue engineering purposes.					
31753363	5	59	theme	sample	875:880	arg1	ratio					868:872	80/20 CS/GG weight ratio	849:872	80/20 CS/GG weight ratio (sample CS/GG80-20)	849:892	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	59	theme	sample	875:880	arg1	CS/GG80-20					882:891	sample CS/GG80-20	875:891	sample CS/GG80-20	875:891	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	4	60	theme	polysaccharide-based	673:692	arg1	scaffolds					694:702	Durable polysaccharide-based scaffolds	665:702	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks	665:764	Durable polysaccharide-based scaffolds with structural homogeneity and interconnecting pore networks are developed by modulating the CS/GG weight ratio.					
31753363	0	61	theme	scaffolding	60:70	arg1	capacity					72:79	the scaffolding capacity	56:79	the scaffolding capacity of hydrogels	56:92	Chitosan/gellan gum ratio content into blends modulates the scaffolding capacity of hydrogels on bone mesenchymal stem cells.					
31753363	5	62	theme	cell	1027:1030	arg1	culture					1032:1038	cell culture	1027:1038	cell culture	1027:1038	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	63	theme	CS/GG	855:859	arg1	ratio					868:872	80/20 CS/GG weight ratio	849:872	80/20 CS/GG weight ratio (sample CS/GG80-20)	849:892	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	5	63	theme	CS/GG	855:859	arg1	CS/GG80-20					882:891	sample CS/GG80-20	875:891	sample CS/GG80-20	875:891	The CS/GG hydrogel prepared at 80/20 CS/GG weight ratio (sample CS/GG80-20) is cytocompatible, supporting the attachment, growth, and spreading of bone marrow mesenchymal stem cells (BMSCs) after nine days of cell culture.					
31753363	7	64	theme	water	1184:1188	arg1	uptake					1190:1195	the water uptake	1180:1195	the water uptake of the material	1180:1211	By controlling the CS content, we can tune the water uptake of the material, enhancing the capacity to serve as a three-dimensional cell scaffold for BMSCs.					
31382820	4	0	theme	Congo	460:464	arg1	staining					470:477	Congo red staining	460:477	Congo red staining	460:477	Its chemical structure was identified using FT-IR, NMR, AFM, SEM and Congo red staining.					
31382820	8	1	theme	IFN-ɑ	1066:1070	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	8	2	from	IgM	1073:1075	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	0	3	theme	polysaccharides	80:94	arg1	Willd					128:132	Willd	128:132	Willd	128:132	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	3	theme	polysaccharides	80:94	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	3	theme	polysaccharides	80:94	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	3	theme	polysaccharides	80:94	arg1	activity					68:75	in vivo immunity-enhancing activity	41:75	in vivo immunity-enhancing activity	41:75	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	3	4	theme	soluble	228:234	arg1	QPS1					272:275	QPS1	272:275	QPS1	272:275	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	3	4	theme	soluble	228:234	arg1	fraction					262:269	The soluble non-starch polysaccharide fraction	224:269	The soluble non-starch polysaccharide fraction (QPS1)	224:276	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	4	5	theme	chemical	395:402	arg1	structure					404:412	Its chemical structure	391:412	Its chemical structure	391:412	Its chemical structure was identified using FT-IR, NMR, AFM, SEM and Congo red staining.					
31382820	0	6	from	Purification	0:11	arg1	quinoa					101:106	quinoa	101:106	quinoa	101:106	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	7	7	theme	immunosuppression	918:934	arg1	improvement					867:877	the improvement	863:877	the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice	863:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	7	8	theme	ICR	939:941	arg1	mice					943:946	ICR mice	939:946	ICR mice	939:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	8	9	from	IFN-ɑ	1066:1070	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	6	10	theme	galacturonic	685:696	arg1	acid					698:701	galacturonic acid	685:701	galacturonic acid	685:701	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	2	11	theme	crude	150:154	arg1	QPS					173:175	QPS	173:175	QPS	173:175	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	2	11	theme	crude	150:154	arg1	polysaccharides					156:170	Quinoa crude polysaccharides	143:170	Quinoa crude polysaccharides (QPS)	143:176	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	8	12	theme	macrophages	1171:1181	arg1	function					1147:1154	the phagocytic function	1132:1154	the phagocytic function of mononuclear macrophages	1132:1181	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	3	13	dep	cellulose	315:323	arg1	chromatography					347:360	gel chromatography	343:360	gel chromatography	343:360	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	2	14	theme	Quinoa	143:148	arg1	QPS					173:175	QPS	173:175	QPS	173:175	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	2	14	theme	Quinoa	143:148	arg1	polysaccharides					156:170	Quinoa crude polysaccharides	143:170	Quinoa crude polysaccharides (QPS)	143:176	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	8	15	theme	phagocytic	1136:1145	arg1	function					1147:1154	the phagocytic function	1132:1154	the phagocytic function of mononuclear macrophages	1132:1181	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	7	16	from	effects	844:850	arg1	improvement					867:877	the improvement	863:877	the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice	863:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	5	17	theme	High	480:483	arg1	chromatography					512:525	High performance gel permeation chromatography	480:525	High performance gel permeation chromatography (HPGPC)	480:533	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	5	17	theme	High	480:483	arg1	HPGPC					528:532	HPGPC	528:532	HPGPC	528:532	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	8	18	theme	mononuclear	1159:1169	arg1	macrophages					1171:1181	mononuclear macrophages	1159:1181	mononuclear macrophages	1159:1181	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	3	19	theme	Sephadex	329:336	arg1	G-50					338:341	Sephadex G-50	329:341	Sephadex G-50	329:341	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	3	20	theme	non-starch	236:245	arg1	QPS1					272:275	QPS1	272:275	QPS1	272:275	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	3	20	theme	non-starch	236:245	arg1	fraction					262:269	The soluble non-starch polysaccharide fraction	224:269	The soluble non-starch polysaccharide fraction (QPS1)	224:276	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	5	21	theme	performance	485:495	arg1	chromatography					512:525	High performance gel permeation chromatography	480:525	High performance gel permeation chromatography (HPGPC)	480:533	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	5	21	theme	performance	485:495	arg1	HPGPC					528:532	HPGPC	528:532	HPGPC	528:532	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	3	22	theme	polysaccharide	247:260	arg1	QPS1					272:275	QPS1	272:275	QPS1	272:275	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	3	22	theme	polysaccharide	247:260	arg1	fraction					262:269	The soluble non-starch polysaccharide fraction	224:269	The soluble non-starch polysaccharide fraction (QPS1)	224:276	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	5	23	theme	molecular	557:565	arg1	weight					567:572	molecular weight	557:572	molecular weight	557:572	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	8	24	theme	immune-enhancing	984:999	arg1	activity					1001:1008	significantly immune-enhancing activity	970:1008	significantly immune-enhancing activity	970:1008	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	7	25	theme	ameliorative	831:842	arg1	effects					844:850	the ameliorative effects	827:850	the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice	827:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	6	26	theme	molar	750:754	arg1	ratio					756:760	a molar ratio	748:760	a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01	748:798	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	3	27	theme	raw	376:378	arg1	materials					380:388	raw materials	376:388	raw materials	376:388	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	3	27	theme	raw	376:378	arg1	QPS					369:371	QPS	369:371	QPS	369:371	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	6	28	theme	34.0 kDa	633:640	arg1	weight					623:628	a molecular weight	611:628	a molecular weight of 34.0 kDa	611:640	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	8	29	theme	delayed	1199:1205	arg1	allergy					1207:1213	delayed allergy	1199:1213	delayed allergy in mice	1199:1221	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	0	30	theme	in	41:42	arg1	activity					68:75	in vivo immunity-enhancing activity	41:75	in vivo immunity-enhancing activity	41:75	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	8	31	theme	IL-6	1060:1063	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	8	32	from	content	1042:1048	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	8	33	from	lysozyme	1081:1088	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	0	34	theme	Chenopodium	109:119	arg1	quinoa					121:126	Chenopodium quinoa	109:126	Chenopodium quinoa Willd.	109:133	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	35	theme	immunity-enhancing	49:66	arg1	activity					68:75	in vivo immunity-enhancing activity	41:75	in vivo immunity-enhancing activity	41:75	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	3	36	theme	DEAE-52	307:313	arg1	cellulose					315:323	DEAE-52 cellulose	307:323	DEAE-52 cellulose	307:323	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	0	37	from	activity	68:75	arg1	quinoa					101:106	quinoa	101:106	quinoa	101:106	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	38	dep	in	41:42	arg1	vivo					44:47	vivo	44:47	vivo	44:47	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	4	39	theme	red	466:468	arg1	staining					470:477	Congo red staining	460:477	Congo red staining	460:477	Its chemical structure was identified using FT-IR, NMR, AFM, SEM and Congo red staining.					
31382820	7	40	from	improvement	867:877	arg1	mice					943:946	ICR mice	939:946	ICR mice	939:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	8	41	from	serum	1100:1104	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	8	42	theme	IFN-γ	1053:1057	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	2	43	theme	quinoa	210:215	arg1	Willd					217:221	Chenopodium quinoa Willd	198:221	Chenopodium quinoa Willd	198:221	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	6	44	theme	2.63:2.40:1.64:6.28:1.95:2.48:5.01	765:798	arg1	ratio					756:760	a molar ratio	748:760	a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01	748:798	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	8	45	theme	IgM	1073:1075	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	2	46	theme	Chenopodium	198:208	arg1	quinoa					210:215	Chenopodium quinoa	198:215	Chenopodium quinoa Willd	198:221	Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd.					
31382820	6	47	theme	molecular	613:621	arg1	weight					623:628	a molecular weight	611:628	a molecular weight of 34.0 kDa	611:640	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	8	48	from	IL-6	1060:1063	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	5	49	theme	gel	497:499	arg1	chromatography					512:525	High performance gel permeation chromatography	480:525	High performance gel permeation chromatography (HPGPC)	480:533	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	5	49	theme	gel	497:499	arg1	HPGPC					528:532	HPGPC	528:532	HPGPC	528:532	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	8	50	theme	lysozyme	1081:1088	arg1	content					1042:1048	the content	1038:1048	the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks	1038:1120	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	0	51	from	quinoa	101:106	arg1	polysaccharides					80:94	polysaccharides	80:94	polysaccharides from quinoa	80:106	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	51	from	quinoa	101:106	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	51	from	quinoa	101:106	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	51	from	quinoa	101:106	arg1	activity					68:75	in vivo immunity-enhancing activity	41:75	in vivo immunity-enhancing activity	41:75	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	51	from	quinoa	101:106	arg1	Willd					128:132	Willd	128:132	Willd	128:132	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	5	52	theme	permeation	501:510	arg1	chromatography					512:525	High performance gel permeation chromatography	480:525	High performance gel permeation chromatography (HPGPC)	480:533	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	5	52	theme	permeation	501:510	arg1	HPGPC					528:532	HPGPC	528:532	HPGPC	528:532	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	7	53	theme	-induced	909:916	arg1	immunosuppression					918:934	anti-cyclophosphamide (CTX)-induced immunosuppression	882:934	anti-cyclophosphamide (CTX)-induced immunosuppression	882:934	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	7	54	theme	QPS1	855:858	arg1	effects					844:850	the ameliorative effects	827:850	the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice	827:946	In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice.					
31382820	8	55	from	IFN-γ	1053:1057	arg1	serum					1100:1104	serum	1100:1104	serum	1100:1104	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	3	56	theme	gel	343:345	arg1	chromatography					347:360	gel chromatography	343:360	gel chromatography	343:360	The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials.					
31382820	0	57	from	elucidation	25:35	arg1	quinoa					101:106	quinoa	101:106	quinoa	101:106	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	0	58	theme	structural	14:23	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.)					
31382820	8	59	from	allergy	1207:1213	arg1	mice					1218:1221	mice	1218:1221	mice	1218:1221	The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.					
31382820	6	60	with	QPS1	600:603	arg1	weight					623:628	a molecular weight	611:628	a molecular weight of 34.0 kDa	611:640	QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01.					
31382820	5	61	used	used	539:542	arg2	HPGPC					528:532	HPGPC	528:532	HPGPC	528:532	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31382820	5	61	used	used	539:542	arg2	chromatography					512:525	High performance gel permeation chromatography	480:525	High performance gel permeation chromatography (HPGPC)	480:533	High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC.					
31518623	0	0	theme	polysaccharides	73:87	arg1	fermentation					24:35	fermentation	24:35	fermentation in vitro	24:44	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	0	theme	polysaccharides	73:87	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	0	theme	polysaccharides	73:87	arg1	plant					169:173	a familiar herbal plant	151:173	a familiar herbal plant	151:173	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	1	1	from	H.	404:405	arg1	digestion					284:292	the simulated digestion	270:292	the simulated digestion (saliva, simulated gastric and small intestinal conditions)	270:352	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	1	from	H.	404:405	arg1	polysaccharides					383:397	polysaccharides	383:397	polysaccharides from H. angustifolia L. (HaLPs)	383:429	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	1	from	H.	404:405	arg1	fermentation					358:369	fermentation	358:369	fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs)	358:429	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	2	2	theme	gastrointestinal	590:605	arg1	digestion					607:615	the simulated gastrointestinal digestion	576:615	the simulated gastrointestinal digestion	576:615	The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely.					
31518623	2	3	contain	had	617:619	arg2	effect					624:629	no effect	621:629	no effect	621:629	The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely.					
31518623	2	3	contain	had	617:619	arg1	digestion					607:615	the simulated gastrointestinal digestion	576:615	the simulated gastrointestinal digestion	576:615	The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely.					
31518623	0	4	from	digestion	10:18	arg1	Helicteres					94:103	Helicteres	94:103	Helicteres	94:103	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	2	5	theme	simulated	580:588	arg1	digestion					607:615	the simulated gastrointestinal digestion	576:615	the simulated gastrointestinal digestion	576:615	The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely.					
31518623	0	6	theme	human	241:245	arg1	health					247:252	human health	241:252	human health	241:252	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	4	7	contain	had	847:849	arg2	effect					881:886	the significantly alternation effect	851:886	the significantly alternation effect on the composition of the gut microbiota	851:927	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	4	7	contain	had	847:849	arg1	HaLPs					841:845	HaLPs	841:845	HaLPs	841:845	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	0	8	theme	various	191:197	arg1	bioactivities					199:211	various bioactivities	191:211	various bioactivities	191:211	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	5	9	theme	total	947:951	arg1	content					977:983	the total short-chain fatty acids content	943:983	the total short-chain fatty acids content	943:983	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	1	10	dep	H.	404:405	arg1	angustifolia					407:418	H. angustifolia	404:418	H. angustifolia L. (HaLPs)	404:429	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	10	dep	H.	404:405	arg1	L					420:420	L	420:420	H. angustifolia L. (HaLPs)	404:429	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	5	11	theme	short-chain	953:963	arg1	acids					971:975	short-chain fatty acids	953:975	the total short-chain fatty acids content	943:983	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	5	12	dep	22.45 ± 4.56 mM	1052:1066	arg1	to					1049:1050	to	1049:1050	to	1049:1050	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	3	13	theme	molecular	703:711	arg1	weight					713:718	the molecular weight	699:718	the molecular weight of HaLPs	699:727	However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions.					
31518623	1	14	from	digestion	284:292	arg1	HaLPs					424:428	HaLPs	424:428	HaLPs	424:428	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	14	from	digestion	284:292	arg1	H.					404:405	H.	404:405	H.	404:405	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	0	15	dep	Helicteres	94:103	arg1	L.					145:146	L.	145:146	L.	145:146	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	15	dep	Helicteres	94:103	arg1	angustifolia					105:116	Helicteres angustifolia L. Helicteres angustifolia L.	94:146	Helicteres angustifolia L. Helicteres angustifolia L.	94:146	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	1	16	theme	high-throughput	510:524	arg1	technology					537:546	high-throughput sequencing technology	510:546	high-throughput sequencing technology	510:546	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	3	17	theme	anaerobic	801:809	arg1	conditions					811:820	anaerobic conditions	801:820	anaerobic conditions	801:820	However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions.					
31518623	5	18	theme	acids	971:975	arg1	content					977:983	the total short-chain fatty acids content	943:983	the total short-chain fatty acids content	943:983	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	1	19	theme	sequencing	526:535	arg1	technology					537:546	high-throughput sequencing technology	510:546	high-throughput sequencing technology	510:546	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	20	from	fermentation	358:369	arg1	HaLPs					424:428	HaLPs	424:428	HaLPs	424:428	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	20	from	fermentation	358:369	arg1	H.					404:405	H.	404:405	H.	404:405	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	0	21	theme	Simulated	0:8	arg1	fermentation					24:35	fermentation	24:35	fermentation in vitro	24:44	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	21	theme	Simulated	0:8	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	21	theme	Simulated	0:8	arg1	plant					169:173	a familiar herbal plant	151:173	a familiar herbal plant	151:173	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	3	22	theme	HaLPs	723:727	arg1	weight					713:718	the molecular weight	699:718	the molecular weight of HaLPs	699:727	However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions.					
31518623	3	22	theme	HaLPs	723:727	arg1	sugar					746:750	the reducing sugar	733:750	the reducing sugar	733:750	However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions.					
31518623	0	23	theme	potential	218:226	arg1	benefits					228:235	potential benefits	218:235	potential benefits for human health	218:252	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	6	24	theme	viable	1167:1172	arg1	basis					1174:1178	a viable basis	1165:1178	a viable basis for their development	1165:1200	These results provided an understanding of the digestive characteristics of HaLPs and afforded a viable basis for their development.					
31518623	1	25	theme	simulated	274:282	arg1	digestion					284:292	the simulated digestion	270:292	the simulated digestion (saliva, simulated gastric and small intestinal conditions)	270:352	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	5	26	theme	fatty	965:969	arg1	acids					971:975	short-chain fatty acids	953:975	the total short-chain fatty acids content	943:983	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	6	27	theme	HaLPs	1146:1150	arg1	characteristics					1127:1141	the digestive characteristics	1113:1141	the digestive characteristics of HaLPs	1113:1150	These results provided an understanding of the digestive characteristics of HaLPs and afforded a viable basis for their development.					
31518623	4	28	theme	microbiota	918:927	arg1	composition					895:905	the composition	891:905	the composition of the gut microbiota	891:927	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	1	29	theme	intestinal	331:340	arg1	conditions					342:351	simulated gastric and small intestinal conditions	303:351	simulated gastric and small intestinal conditions	303:351	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	29	theme	intestinal	331:340	arg1	saliva					295:300	saliva	295:300	saliva	295:300	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	30	from	effects	455:461	arg1	microbiota					479:488	gut microbiota	475:488	gut microbiota	475:488	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	4	31	theme	gut	914:916	arg1	microbiota					918:927	the gut microbiota	910:927	the gut microbiota	910:927	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	1	32	theme	polysaccharides	383:397	arg1	digestion					284:292	the simulated digestion	270:292	the simulated digestion (saliva, simulated gastric and small intestinal conditions)	270:352	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	32	theme	polysaccharides	383:397	arg1	fermentation					358:369	fermentation	358:369	fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs)	358:429	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	3	33	theme	reducing	737:744	arg1	sugar					746:750	the reducing sugar	733:750	the reducing sugar	733:750	However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions.					
31518623	0	34	theme	human	49:53	arg1	microbiota					59:68	human gut microbiota	49:68	human gut microbiota	49:68	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	35	theme	familiar	153:160	arg1	fermentation					24:35	fermentation	24:35	fermentation in vitro	24:44	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	35	theme	familiar	153:160	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	35	theme	familiar	153:160	arg1	plant					169:173	a familiar herbal plant	151:173	a familiar herbal plant	151:173	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	1	36	dep	digestion	284:292	arg1	conditions					342:351	simulated gastric and small intestinal conditions	303:351	simulated gastric and small intestinal conditions	303:351	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	36	dep	digestion	284:292	arg1	saliva					295:300	saliva	295:300	saliva	295:300	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	37	theme	HaLPs	466:470	arg1	effects					455:461	the effects	451:461	the effects of HaLPs on gut microbiota	451:488	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	4	38	theme	alternation	869:879	arg1	effect					881:886	the significantly alternation effect	851:886	the significantly alternation effect on the composition of the gut microbiota	851:927	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	1	39	theme	gastric	313:319	arg1	conditions					342:351	simulated gastric and small intestinal conditions	303:351	simulated gastric and small intestinal conditions	303:351	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	1	39	theme	gastric	313:319	arg1	saliva					295:300	saliva	295:300	saliva	295:300	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	2	40	theme	large	666:670	arg1	intestine					672:680	the large intestine	662:680	the large intestine	662:680	The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely.					
31518623	5	41	dep	increased	985:993	arg1	22.45 ± 4.56 mM					1052:1066	22.45 ± 4.56 mM	1052:1066	22.45 ± 4.56 mM	1052:1066	Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM).					
31518623	0	42	theme	herbal	162:167	arg1	fermentation					24:35	fermentation	24:35	fermentation in vitro	24:44	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	42	theme	herbal	162:167	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	42	theme	herbal	162:167	arg1	plant					169:173	a familiar herbal plant	151:173	a familiar herbal plant	151:173	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	43	from	Helicteres	94:103	arg1	fermentation					24:35	fermentation	24:35	fermentation in vitro	24:44	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	43	from	Helicteres	94:103	arg1	plant					169:173	a familiar herbal plant	151:173	a familiar herbal plant	151:173	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	43	from	Helicteres	94:103	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	43	from	Helicteres	94:103	arg1	polysaccharides					73:87	polysaccharides	73:87	polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L.	73:146	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	1	44	theme	gut	475:477	arg1	microbiota					479:488	gut microbiota	475:488	gut microbiota	475:488	In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology.					
31518623	6	45	theme	characteristics	1127:1141	arg1	understanding					1096:1108	an understanding	1093:1108	an understanding of the digestive characteristics of HaLPs	1093:1150	These results provided an understanding of the digestive characteristics of HaLPs and afforded a viable basis for their development.					
31518623	4	46	from	effect	881:886	arg1	composition					895:905	the composition	891:905	the composition of the gut microbiota	891:927	It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota.					
31518623	6	47	theme	digestive	1117:1125	arg1	characteristics					1127:1141	the digestive characteristics	1113:1141	the digestive characteristics of HaLPs	1113:1150	These results provided an understanding of the digestive characteristics of HaLPs and afforded a viable basis for their development.					
31518623	0	48	theme	gut	55:57	arg1	microbiota					59:68	human gut microbiota	49:68	human gut microbiota	49:68	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
31518623	0	49	from	fermentation	24:35	arg1	Helicteres					94:103	Helicteres	94:103	Helicteres	94:103	Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L. Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health.					
30934822	0	0	theme	Defoamed	74:81	arg1	Beer					83:86	Defoamed Beer	74:86	Defoamed Beer	74:86	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	8	1	theme	irregular	1284:1292	arg1	lamellas					1294:1301	irregular lamellas	1284:1301	irregular lamellas	1284:1301	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	3	2	theme	polymerization	520:533	arg1	degrees					509:515	average degrees	501:515	average degrees of polymerization (avDP) of arabinoxylan	501:556	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	3	2	theme	polymerization	520:533	arg1	composition					442:452	The monosaccharide composition	423:452	The monosaccharide composition	423:452	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	3	2	theme	polymerization	520:533	arg1	degree					468:473	substitution degree	455:473	substitution degree (Ara/Xyl ratio, A/X)	455:494	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	5	3	from	content	735:741	arg1	BF					750:751	the BF	746:751	the BF	746:751	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	1	4	theme	molecular	144:152	arg1	characterization					154:169	some molecular characterization	139:169	some molecular characterization	139:169	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	10	5	theme	foam	1707:1710	arg1	characteristics					1712:1726	wheat beer foam characteristics	1696:1726	wheat beer foam characteristics	1696:1726	It was suggested that the arabinoxylan played important roles in maintaining wheat beer foam characteristics.					
30934822	8	6	theme	arabinoxylan	1376:1387	arg1	structures					1317:1326	spherical structures	1307:1326	spherical structures	1307:1326	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	6	theme	arabinoxylan	1376:1387	arg1	weight					1361:1366	the weight-average molecular weight	1332:1366	the weight-average molecular weight (Mw) of arabinoxylan	1332:1387	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	6	theme	arabinoxylan	1376:1387	arg1	Mw					1369:1370	Mw	1369:1370	Mw	1369:1370	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	6	theme	arabinoxylan	1376:1387	arg1	lamellas					1294:1301	irregular lamellas	1284:1301	irregular lamellas	1284:1301	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	9	7	theme	beer	1512:1515	arg1	foam					1517:1520	beer foam	1512:1520	beer foam	1512:1520	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	1	8	theme	arabinoxylan	281:292	arg1	influences					267:276	the crucial influences	255:276	the crucial influences of arabinoxylan on wheat beer and its foam	255:319	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	9	9	theme	%	1539:1539	arg1	ethanol					1541:1547	50% ethanol	1537:1547	50% ethanol	1537:1547	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	8	10	theme	largest	1431:1437	arg1	values					1439:1444	the largest values	1427:1444	the largest values	1427:1444	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	11	theme	lowest	1400:1405	arg1	values					1407:1412	the lowest values	1396:1412	the lowest values in BF	1396:1418	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	2	12	theme	%	406:406	arg1	ethanol					388:394	ethanol	388:394	ethanol of 50%, 67%, 75%, and 80%	388:420	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	6	13	theme	highest	965:971	arg1	value					973:977	highest value	965:977	highest value of avDP of the arabinoxylan	965:1005	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	5	14	from	precipitated	809:820	arg1	fraction					828:835	BF fraction	825:835	BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides	825:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	1	15	theme	wheat	297:301	arg1	beer					303:306	wheat beer	297:306	wheat beer	297:306	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	9	16	theme	50	1537:1538	arg1	%					1539:1539	%	1539:1539	%	1539:1539	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	5	17	theme	same	690:693	arg1	concentration					703:715	the same ethanol concentration	686:715	the same ethanol concentration	686:715	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	18	with	fraction	828:835	arg1	ethanol					846:852	50% ethanol	842:852	50% ethanol which accounted for 80.84% of the total polysaccharides	842:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	19	theme	polysaccharides	894:908	arg1	polysaccharides					894:908	the total polysaccharides	884:908	the total polysaccharides	884:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	19	theme	polysaccharides	894:908	arg1	%					879:879	80.84%	874:879	80.84% of the total polysaccharides	874:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	6	20	theme	foam	1029:1032	arg1	fractions					1034:1042	all beer foam fractions	1020:1042	all beer foam fractions	1020:1042	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	8	21	from	values	1407:1412	arg1	BF					1417:1418	BF	1417:1418	BF	1417:1418	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	5	22	theme	total	888:892	arg1	polysaccharides					894:908	the total polysaccharides	884:908	the total polysaccharides	884:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	23	theme	ethanol	695:701	arg1	concentration					703:715	the same ethanol concentration	686:715	the same ethanol concentration	686:715	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	3	24	theme	arabinoxylan	545:556	arg1	avDP					536:539	avDP	536:539	avDP	536:539	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	3	24	theme	arabinoxylan	545:556	arg1	polymerization					520:533	polymerization	520:533	polymerization (avDP) of arabinoxylan	520:556	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	6	25	theme	beer	1024:1027	arg1	fractions					1034:1042	all beer foam fractions	1020:1042	all beer foam fractions	1020:1042	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	8	26	theme	spherical	1307:1315	arg1	structures					1317:1326	spherical structures	1307:1326	spherical structures	1307:1326	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	4	27	theme	Molecular	577:585	arg1	weight					587:592	Molecular weight	577:592	Molecular weight	577:592	Molecular weight and microstructure were also involved in this study by GPC-LLS and SEM, respectively.					
30934822	0	28	from	Beer	54:57	arg1	Characterization					10:25	Molecular Characterization	0:25	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.	0:87	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	1	29	theme	arabinoxylan	174:185	arg1	characterization					154:169	some molecular characterization	139:169	some molecular characterization	139:169	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	29	theme	arabinoxylan	174:185	arg1	distribution					122:133	distribution	122:133	distribution	122:133	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	0	30	theme	Molecular	0:8	arg1	Characterization					10:25	Molecular Characterization	0:25	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.	0:87	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	1	31	theme	defoamed	225:232	arg1	DB					240:241	DB	240:241	DB	240:241	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	31	theme	defoamed	225:232	arg1	beer					234:237	defoamed beer	225:237	defoamed beer (DB)	225:242	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	3	32	theme	average	501:507	arg1	degrees					509:515	average degrees	501:515	average degrees of polymerization (avDP) of arabinoxylan	501:556	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	7	33	theme	p	1190:1190	arg1	difference					1178:1187	a significant difference	1164:1187	a significant difference (p < 0.05) among B, BF, and DB	1164:1218	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	7	33	theme	p	1190:1190	arg1	<					1192:1192	p < 0.05	1190:1197	p < 0.05	1190:1197	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	7	34	theme	average	1097:1103	arg1	degrees					1105:1111	The average degrees	1093:1111	The average degrees of polymerization (avDP) of arabinoxylan	1093:1152	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	0	35	theme	Arabinoxylan	30:41	arg1	Characterization					10:25	Molecular Characterization	0:25	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.	0:87	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	7	36	theme	polymerization	1116:1129	arg1	degrees					1105:1111	The average degrees	1093:1111	The average degrees of polymerization (avDP) of arabinoxylan	1093:1152	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	6	37	theme	arabinoxylan	994:1005	arg1	avDP					982:985	avDP	982:985	avDP of the arabinoxylan	982:1005	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	1	38	dep	distribution	122:133	arg1	the					118:120	the	118:120	the	118:120	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	39	theme	wheat	190:194	arg1	beer					196:199	wheat beer	190:199	wheat beer (B)	190:203	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	39	theme	wheat	190:194	arg1	B					202:202	B	202:202	B	202:202	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	2	40	from	DB	364:365	arg1	arabinoxylan					335:346	The purified arabinoxylan	322:346	The purified arabinoxylan from B, BF, and DB	322:365	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	6	41	theme	substitution	935:946	arg1	A/X					956:958	A/X	956:958	A/X	956:958	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	41	theme	substitution	935:946	arg1	degree					948:953	the greatest substitution degree	922:953	the greatest substitution degree (A/X)	922:959	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	0	42	theme	Wheat	48:52	arg1	Beer					54:57	Wheat Beer	48:57	Wheat Beer	48:57	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	9	43	theme	lower	1609:1613	arg1	Mw					1615:1616	lower Mw	1609:1616	lower Mw	1609:1616	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	2	44	theme	%	411:411	arg1	ethanol					388:394	ethanol	388:394	ethanol of 50%, 67%, 75%, and 80%	388:420	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	5	45	theme	BF	825:826	arg1	fraction					828:835	BF fraction	825:835	BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides	825:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	46	from	fraction	828:835	arg1	precipitated					809:820	precipitated	809:820	precipitated	809:820	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	10	47	theme	wheat	1696:1700	arg1	characteristics					1712:1726	wheat beer foam characteristics	1696:1726	wheat beer foam characteristics	1696:1726	It was suggested that the arabinoxylan played important roles in maintaining wheat beer foam characteristics.					
30934822	6	48	located	found	1011:1015	arg2	value					973:977	highest value	965:977	highest value of avDP of the arabinoxylan	965:1005	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	48	located	found	1011:1015	arg1	fractions					1034:1042	all beer foam fractions	1020:1042	all beer foam fractions	1020:1042	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	48	located	found	1011:1015	arg2	A/X					956:958	A/X	956:958	A/X	956:958	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	48	located	found	1011:1015	arg2	Meanwhile					911:919	Meanwhile	911:919	Meanwhile	911:919	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	48	located	found	1011:1015	arg2	degree					948:953	the greatest substitution degree	922:953	the greatest substitution degree (A/X)	922:959	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	0	49	from	Foam	65:68	arg1	Characterization					10:25	Molecular Characterization	0:25	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.	0:87	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	3	50	theme	substitution	455:466	arg1	degree					468:473	substitution degree	455:473	substitution degree (Ara/Xyl ratio, A/X)	455:494	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	7	51	theme	arabinoxylan	1141:1152	arg1	avDP					1132:1135	avDP	1132:1135	avDP	1132:1135	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	7	51	theme	arabinoxylan	1141:1152	arg1	polymerization					1116:1129	polymerization	1116:1129	polymerization (avDP) of arabinoxylan	1116:1152	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	2	52	theme	purified	326:333	arg1	arabinoxylan					335:346	The purified arabinoxylan	322:346	The purified arabinoxylan from B, BF, and DB	322:365	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	8	53	theme	weight-average	1336:1349	arg1	Mw					1369:1370	Mw	1369:1370	Mw	1369:1370	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	53	theme	weight-average	1336:1349	arg1	weight					1361:1366	the weight-average molecular weight	1332:1366	the weight-average molecular weight (Mw) of arabinoxylan	1332:1387	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	3	54	theme	monosaccharide	427:440	arg1	composition					442:452	The monosaccharide composition	423:452	The monosaccharide composition	423:452	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	6	55	theme	ethanol	1079:1085	arg1	concentration					1062:1074	the concentration	1058:1074	the concentration of ethanol used	1058:1090	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	56	theme	greatest	926:933	arg1	A/X					956:958	A/X	956:958	A/X	956:958	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	56	theme	greatest	926:933	arg1	degree					948:953	the greatest substitution degree	922:953	the greatest substitution degree (A/X)	922:959	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	1	57	from	influences	267:276	arg1	beer					303:306	wheat beer	297:306	wheat beer	297:306	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	57	from	influences	267:276	arg1	foam					316:319	its foam	312:319	its foam	312:319	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	2	58	from	BF	356:357	arg1	arabinoxylan					335:346	The purified arabinoxylan	322:346	The purified arabinoxylan from B, BF, and DB	322:365	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	5	59	theme	%	844:844	arg1	ethanol					846:852	50% ethanol	842:852	50% ethanol which accounted for 80.84% of the total polysaccharides	842:908	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	60	theme	50	842:843	arg1	%					844:844	%	844:844	%	844:844	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	10	61	theme	important	1665:1673	arg1	roles					1675:1679	important roles	1665:1679	important roles	1665:1679	It was suggested that the arabinoxylan played important roles in maintaining wheat beer foam characteristics.					
30934822	8	62	theme	molecular	1351:1359	arg1	Mw					1369:1370	Mw	1369:1370	Mw	1369:1370	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	8	62	theme	molecular	1351:1359	arg1	weight					1361:1366	the weight-average molecular weight	1332:1366	the weight-average molecular weight (Mw) of arabinoxylan	1332:1387	Furthermore, arabinoxylan presented varied microstructure with irregular lamellas and spherical structures and the weight-average molecular weight (Mw) of arabinoxylan showed the lowest values in BF, while the largest values were shown in DB.					
30934822	2	63	theme	%	420:420	arg1	ethanol					388:394	ethanol	388:394	ethanol of 50%, 67%, 75%, and 80%	388:420	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	3	64	theme	Ara/Xyl	476:482	arg1	A/X					491:493	A/X	491:493	A/X	491:493	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	3	64	theme	Ara/Xyl	476:482	arg1	ratio					484:488	Ara/Xyl ratio	476:488	Ara/Xyl ratio	476:488	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	9	65	theme	greater	1567:1573	arg1	value					1575:1579	greater value	1567:1579	greater value of A/X and avDP, as well as lower Mw	1567:1616	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	7	66	theme	significant	1166:1176	arg1	difference					1178:1187	a significant difference	1164:1187	a significant difference (p < 0.05) among B, BF, and DB	1164:1218	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	7	66	theme	significant	1166:1176	arg1	<					1192:1192	p < 0.05	1190:1197	p < 0.05	1190:1197	The average degrees of polymerization (avDP) of arabinoxylan displayed a significant difference (p < 0.05) among B, BF, and DB.					
30934822	9	67	theme	A/X	1584:1586	arg1	value					1575:1579	greater value	1567:1579	greater value of A/X and avDP, as well as lower Mw	1567:1616	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	9	68	theme	Mw	1615:1616	arg1	value					1575:1579	greater value	1567:1579	greater value of A/X and avDP, as well as lower Mw	1567:1616	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	2	69	theme	%	401:401	arg1	ethanol					388:394	ethanol	388:394	ethanol of 50%, 67%, 75%, and 80%	388:420	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	0	70	from	Beer	83:86	arg1	Characterization					10:25	Molecular Characterization	0:25	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.	0:87	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	6	71	theme	avDP	982:985	arg1	A/X					956:958	A/X	956:958	A/X	956:958	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	71	theme	avDP	982:985	arg1	degree					948:953	the greatest substitution degree	922:953	the greatest substitution degree (A/X)	922:959	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	71	theme	avDP	982:985	arg1	Meanwhile					911:919	Meanwhile	911:919	Meanwhile	911:919	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	6	71	theme	avDP	982:985	arg1	value					973:977	highest value	965:977	highest value of avDP of the arabinoxylan	965:1005	Meanwhile, the greatest substitution degree (A/X) and highest value of avDP of the arabinoxylan was found in all beer foam fractions regardless of the concentration of ethanol used.					
30934822	3	72	dep	degree	468:473	arg1	A/X					491:493	A/X	491:493	A/X	491:493	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	3	72	dep	degree	468:473	arg1	ratio					484:488	Ara/Xyl ratio	476:488	Ara/Xyl ratio	476:488	The monosaccharide composition, substitution degree (Ara/Xyl ratio, A/X), and average degrees of polymerization (avDP) of arabinoxylan were investigated.					
30934822	1	73	theme	beer	206:209	arg1	BF					217:218	BF	217:218	BF	217:218	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	1	73	theme	beer	206:209	arg1	foam					211:214	beer foam	206:214	beer foam (BF)	206:219	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	0	74	theme	Beer	60:63	arg1	Foam					65:68	Beer Foam	60:68	Beer Foam	60:68	Molecular Characterization of Arabinoxylan from Wheat Beer, Beer Foam and Defoamed Beer.					
30934822	1	75	theme	crucial	259:265	arg1	influences					267:276	the crucial influences	255:276	the crucial influences of arabinoxylan on wheat beer and its foam	255:319	This research was to explore the distribution and some molecular characterization of arabinoxylan in wheat beer (B), beer foam (BF) and defoamed beer (DB) because of the crucial influences of arabinoxylan on wheat beer and its foam.					
30934822	2	76	from	B	353:353	arg1	arabinoxylan					335:346	The purified arabinoxylan	322:346	The purified arabinoxylan from B, BF, and DB	322:365	The purified arabinoxylan from B, BF, and DB were fractionated by ethanol of 50%, 67%, 75%, and 80%.					
30934822	5	77	theme	arabinoxylan	722:733	arg1	content					735:741	the arabinoxylan content	718:741	the arabinoxylan content in the BF	718:751	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	5	77	theme	arabinoxylan	722:733	arg1	higher					757:762	higher	757:762	higher	757:762	Under the same ethanol concentration, the arabinoxylan content in the BF was higher than the other two, respectively, and it was precipitated in BF fraction with 50% ethanol which accounted for 80.84% of the total polysaccharides.					
30934822	9	78	theme	avDP	1592:1595	arg1	value					1575:1579	greater value	1567:1579	greater value of A/X and avDP, as well as lower Mw	1567:1616	Therefore, arabinoxylan was more accumulated in beer foam, especially in 50% ethanol, characterised by greater value of A/X and avDP, as well as lower Mw.					
30934822	10	79	theme	beer	1702:1705	arg1	characteristics					1712:1726	wheat beer foam characteristics	1696:1726	wheat beer foam characteristics	1696:1726	It was suggested that the arabinoxylan played important roles in maintaining wheat beer foam characteristics.					
29386647	3	0	theme	synthetic	748:756	arg1	UDP-CH2-GalNAc					775:788	synthetic, nonhydrolyzable UDP-CH2-GalNAc	748:788	UDP-CH2-GalNAc	775:788	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	4	1	theme	glycan	903:908	arg1	counting					910:917	glycan counting	903:917	glycan counting	903:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	0	2	theme	glycosyltransferase	65:83	arg1	mechanism					40:48	the molecular ruler mechanism	20:48	the molecular ruler mechanism of a bacterial glycosyltransferase	20:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	1	3	link	lipid-linked	230:241	arg1	LLO					260:262	LLO	260:262	LLO	260:262	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	3	link	lipid-linked	230:241	arg1	oligosaccharide					243:257	the lipid-linked oligosaccharide	226:257	the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	226:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	3	link	lipid-linked	230:241	arg1	donor					291:295	the glycan donor	280:295	the glycan donor in bacterial protein N-glycosylation	280:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	4	from	jejuni	180:185	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	4	from	jejuni	180:185	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	3	5	from	complexes	666:674	arg1	complex					626:632	a binary complex	617:632	a binary complex with UDP-GalNAc	617:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	5	from	complexes	666:674	arg1	states					599:604	three distinct states	584:604	three distinct states	584:604	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	4	6	theme	helix	834:838	arg1	"					839:839	"ruler helix"	827:839	"ruler helix"	827:839	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	6	theme	helix	834:838	arg1	helix					820:824	an amphipathic helix	805:824	an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting	805:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	5	7	theme	LLO	1027:1029	arg1	substrate					1031:1039	the LLO substrate	1023:1039	the LLO substrate	1023:1039	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	4	8	contain	has	847:849	arg2	role					858:861	a dual role	851:861	a dual role of facilitating membrane attachment and glycan counting	851:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	8	contain	has	847:849	arg1	"					839:839	"ruler helix"	827:839	"ruler helix"	827:839	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	8	contain	has	847:849	arg1	helix					820:824	an amphipathic helix	805:824	an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting	805:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	6	9	theme	glycan	1181:1186	arg1	counting					1188:1195	glycan counting	1181:1195	glycan counting	1181:1195	These results, combined with molecular dynamics simulations, provide the mechanism of glycan counting by PglH.					
29386647	5	10	theme	pyrophosphate	1000:1012	arg1	substrate					1031:1039	the LLO substrate	1023:1039	the LLO substrate	1023:1039	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	10	theme	pyrophosphate	1000:1012	arg1	group					1014:1018	the pyrophosphate group	996:1018	the pyrophosphate group of the LLO substrate	996:1039	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	11	theme	side	970:973	arg1	chains					975:980	three positively charged side chains	945:980	three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three	945:1092	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	3	12	with	complex	626:632	arg1	UDP-GalNAc					639:648	UDP-GalNAc	639:648	UDP-GalNAc	639:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	13	from	structures	562:571	arg1	complex					626:632	a binary complex	617:632	a binary complex with UDP-GalNAc	617:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	13	from	structures	562:571	arg1	states					599:604	three distinct states	584:604	three distinct states	584:604	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	4	14	theme	ruler	828:832	arg1	"					839:839	"ruler helix"	827:839	"ruler helix"	827:839	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	14	theme	ruler	828:832	arg1	helix					820:824	an amphipathic helix	805:824	an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting	805:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	5	15	theme	charged	962:968	arg1	chains					975:980	three positively charged side chains	945:980	three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three	945:1092	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	2	16	theme	growing	466:472	arg1	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl					489:534	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl	489:534	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl	489:534	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	2	16	theme	growing	466:472	arg1	precursor					478:486	the growing LLO precursor	462:486	the growing LLO precursor	462:486	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	3	17	theme	PglH	576:579	arg1	complexes					666:674	two ternary complexes	654:674	two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc	654:788	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	17	theme	PglH	576:579	arg1	structures					562:571	crystal structures	554:571	crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc	554:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	18	theme	nonhydrolyzable	759:773	arg1	UDP-CH2-GalNAc					775:788	synthetic, nonhydrolyzable UDP-CH2-GalNAc	748:788	UDP-CH2-GalNAc	775:788	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	19	theme	LLO	719:721	arg1	analog					723:728	a chemo-enzymatically generated LLO analog	687:728	a chemo-enzymatically generated LLO analog	687:728	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	0	20	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase	0:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	1	21	theme	glycan	284:289	arg1	oligosaccharide					243:257	the lipid-linked oligosaccharide	226:257	the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	226:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	21	theme	glycan	284:289	arg1	donor					291:295	the glycan donor	280:295	the glycan donor in bacterial protein N-glycosylation	280:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	22	from	donor	291:295	arg1	N-glycosylation					318:332	bacterial protein N-glycosylation	300:332	bacterial protein N-glycosylation	300:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	23	theme	membrane-associated	90:108	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	23	theme	membrane-associated	90:108	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	5	24	dep	bind	991:994	arg1	limit					1050:1054	limit	1050:1054	limit the addition of GalNAc units to three	1050:1092	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	1	25	theme	biosynthetic	202:213	arg1	pathway					215:221	the biosynthetic pathway	198:221	the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	198:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	2	26	theme	units	453:457	arg1	transfer					391:398	the transfer	387:398	the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl	387:534	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	4	27	theme	dual	853:856	arg1	role					858:861	a dual role	851:861	a dual role of facilitating membrane attachment and glycan counting	851:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	3	28	theme	distinct	590:597	arg1	complex					626:632	a binary complex	617:632	a binary complex with UDP-GalNAc	617:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	28	theme	distinct	590:597	arg1	states					599:604	three distinct states	584:604	three distinct states	584:604	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	1	29	theme	pathway	215:221	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	29	theme	pathway	215:221	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	6	30	theme	dynamics	1134:1141	arg1	simulations					1143:1153	molecular dynamics simulations	1124:1153	molecular dynamics simulations	1124:1153	These results, combined with molecular dynamics simulations, provide the mechanism of glycan counting by PglH.					
29386647	4	31	contain	contains	796:803	arg1	PglH					791:794	PglH	791:794	PglH	791:794	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	31	contain	contains	796:803	arg2	helix					820:824	an amphipathic helix	805:824	an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting	805:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	31	contain	contains	796:803	arg2	"					839:839	"ruler helix"	827:839	"ruler helix"	827:839	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	1	32	theme	processive	111:120	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	32	theme	processive	111:120	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	6	33	theme	molecular	1124:1132	arg1	simulations					1143:1153	molecular dynamics simulations	1124:1153	molecular dynamics simulations	1124:1153	These results, combined with molecular dynamics simulations, provide the mechanism of glycan counting by PglH.					
29386647	0	34	theme	ruler	34:38	arg1	mechanism					40:48	the molecular ruler mechanism	20:48	the molecular ruler mechanism of a bacterial glycosyltransferase	20:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	1	35	theme	bacterial	300:308	arg1	N-glycosylation					318:332	bacterial protein N-glycosylation	300:332	bacterial protein N-glycosylation	300:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	3	36	contain	containing	676:685	arg1	complexes					666:674	two ternary complexes	654:674	two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc	654:788	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	36	contain	containing	676:685	arg2	analog					723:728	a chemo-enzymatically generated LLO analog	687:728	a chemo-enzymatically generated LLO analog	687:728	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	36	contain	containing	676:685	arg2	UDP					741:743	UDP	741:743	UDP	741:743	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	2	37	theme	N-acetylgalactosamine	422:442	arg1	units					453:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	0	38	theme	molecular	24:32	arg1	mechanism					40:48	the molecular ruler mechanism	20:48	the molecular ruler mechanism of a bacterial glycosyltransferase	20:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	1	39	theme	protein	310:316	arg1	N-glycosylation					318:332	bacterial protein N-glycosylation	300:332	bacterial protein N-glycosylation	300:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	40	theme	lipid-linked	230:241	arg1	LLO					260:262	LLO	260:262	LLO	260:262	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	40	theme	lipid-linked	230:241	arg1	oligosaccharide					243:257	the lipid-linked oligosaccharide	226:257	the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	226:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	40	theme	lipid-linked	230:241	arg1	donor					291:295	the glycan donor	280:295	the glycan donor in bacterial protein N-glycosylation	280:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	2	41	theme	LLO	474:476	arg1	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl					489:534	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl	489:534	GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl	489:534	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	2	41	theme	LLO	474:476	arg1	precursor					478:486	the growing LLO precursor	462:486	the growing LLO precursor	462:486	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	4	42	theme	membrane	879:886	arg1	attachment					888:897	membrane attachment	879:897	membrane attachment	879:897	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	0	43	theme	mechanism	40:48	arg1	basis					11:15	Structural basis	0:15	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase	0:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	3	44	theme	binary	619:624	arg1	complex					626:632	a binary complex	617:632	a binary complex with UDP-GalNAc	617:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	1	45	theme	retaining	126:134	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	45	theme	retaining	126:134	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	5	46	theme	GalNAc	1072:1077	arg1	units					1079:1083	GalNAc units	1072:1083	GalNAc units	1072:1083	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	1	47	theme	glycosyltransferase	136:154	arg1	part					190:193	part	190:193	part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	190:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	1	47	theme	glycosyltransferase	136:154	arg1	PglH					156:159	The membrane-associated, processive and retaining glycosyltransferase PglH	86:159	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni	86:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	4	48	theme	amphipathic	808:818	arg1	"					839:839	"ruler helix"	827:839	"ruler helix"	827:839	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	4	48	theme	amphipathic	808:818	arg1	helix					820:824	an amphipathic helix	805:824	an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting	805:917	PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting.					
29386647	1	49	theme	oligosaccharide	243:257	arg1	pathway					215:221	the biosynthetic pathway	198:221	the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation	198:332	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
29386647	6	50	theme	counting	1188:1195	arg1	mechanism					1168:1176	the mechanism	1164:1176	the mechanism of glycan counting by PglH	1164:1203	These results, combined with molecular dynamics simulations, provide the mechanism of glycan counting by PglH.					
29386647	3	51	theme	generated	709:717	arg1	analog					723:728	a chemo-enzymatically generated LLO analog	687:728	a chemo-enzymatically generated LLO analog	687:728	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	3	52	theme	ternary	658:664	arg1	complexes					666:674	two ternary complexes	654:674	two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc	654:788	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	5	53	contain	contains	936:943	arg1	helix					930:934	The ruler helix	920:934	The ruler helix	920:934	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	53	contain	contains	936:943	arg2	chains					975:980	three positively charged side chains	945:980	three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three	945:1092	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	54	theme	ruler	924:928	arg1	helix					930:934	The ruler helix	920:934	The ruler helix	920:934	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	55	theme	units	1079:1083	arg1	addition					1060:1067	the addition	1056:1067	the addition of GalNAc units to three	1056:1092	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	2	56	theme	unknown	344:350	arg1	mechanism					361:369	an unknown counting mechanism	341:369	an unknown counting mechanism	341:369	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	0	57	theme	bacterial	55:63	arg1	glycosyltransferase					65:83	a bacterial glycosyltransferase	53:83	a bacterial glycosyltransferase	53:83	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.					
29386647	2	58	theme	α1,4	417:420	arg1	units					453:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	5	59	theme	substrate	1031:1039	arg1	substrate					1031:1039	the LLO substrate	1023:1039	the LLO substrate	1023:1039	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	5	59	theme	substrate	1031:1039	arg1	group					1014:1018	the pyrophosphate group	996:1018	the pyrophosphate group of the LLO substrate	996:1039	The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three.					
29386647	2	60	theme	GalNAc	445:450	arg1	units					453:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	exactly three α1,4 N-acetylgalactosamine (GalNAc) units	403:457	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	3	61	theme	crystal	554:560	arg1	structures					562:571	crystal structures	554:571	crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc	554:648	Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc.					
29386647	2	62	theme	counting	352:359	arg1	mechanism					361:369	an unknown counting mechanism	341:369	an unknown counting mechanism	341:369	Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl.					
29386647	1	63	theme	Campylobacter	166:178	arg1	jejuni					180:185	Campylobacter jejuni	166:185	Campylobacter jejuni	166:185	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation.					
30980422	8	0	theme	intracellular	1193:1205	arg1	compartments					1207:1218	distinct intracellular compartments	1184:1218	distinct intracellular compartments	1184:1218	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	7	1	from	role	1103:1106	arg1	process					1136:1142	this process	1131:1142	this process	1131:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	6	2	theme	unreported	809:818	arg1	mode					820:823	a previously unreported mode	796:823	a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage	796:903	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	9	3	theme	water	1470:1474	arg1	storage					1476:1482	water storage	1470:1482	water storage	1470:1482	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	8	4	theme	pectins	1278:1284	arg1	distribution					1157:1168	The in situ distribution	1145:1168	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage	1145:1246	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	8	4	theme	pectins	1278:1284	arg1	upregulation					1262:1273	apparent upregulation	1253:1273	apparent upregulation of pectins, imparting flexibility to the cell wall,	1253:1325	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	5	theme	leaf-succulent	611:624	arg1	species					647:653	leaf-succulent Aloe (Asphodelaceae) species	611:653	leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling	611:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	6	6	theme	water	890:894	arg1	shortage					896:903	water shortage	890:903	water shortage	890:903	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	8	7	dep	in	1149:1150	arg1	situ					1152:1155	situ	1152:1155	situ	1152:1155	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	7	8	from	mechanics	1038:1046	arg1	role					1103:1106	the possible role	1090:1106	the possible role of homogalacturonan in this process	1090:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	7	8	from	mechanics	1038:1046	arg1	remobilization					1055:1068	the remobilization	1051:1068	the remobilization of stored water	1051:1084	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	8	9	theme	cell	1316:1319	arg1	wall					1321:1324	the cell wall	1312:1324	the cell wall	1312:1324	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	7	10	theme	possible	1094:1101	arg1	role					1103:1106	the possible role	1090:1106	the possible role of homogalacturonan in this process	1090:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	2	11	contain	have	202:205	arg1	plants					195:200	Succulent plants	185:200	Succulent plants	185:200	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	2	11	contain	have	202:205	arg2	capacity					211:218	the capacity to tolerate periodically dry environments	207:260	the capacity to tolerate periodically dry environments	207:260	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	7	12	theme	mechanics	1038:1046	arg1	indicative					1000:1009	indicative	1000:1009	indicative	1000:1009	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	7	13	theme	stored	1073:1078	arg1	water					1080:1084	stored water	1073:1084	stored water	1073:1084	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	7	14	theme	cell	1028:1031	arg1	mechanics					1038:1046	predetermined cell wall mechanics	1014:1046	predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	1014:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	8	15	theme	wall	1353:1356	arg1	folding					1358:1364	elaborate cell wall folding	1338:1364	elaborate cell wall folding	1338:1364	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	16	theme	microarray	754:763	arg1	profiling					773:781	comprehensive microarray polymer profiling	740:781	comprehensive microarray polymer profiling	740:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	3	17	from	components	385:394	arg1	cells					428:432	hydrenchyma cells	416:432	hydrenchyma cells	416:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	2	18	theme	periodically	232:243	arg1	environments					249:260	periodically dry environments	232:260	periodically dry environments	232:260	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	5	19	theme	histological	678:689	arg1	microscopy					691:700	histological microscopy	678:700	histological microscopy	678:700	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	0	20	theme	drought	62:68	arg1	responses					70:78	the drought responses	58:78	the drought responses of succulent Aloe species	58:104	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	1	21	theme	adaptations	142:152	arg1	multitude					129:137	a multitude	127:137	a multitude of adaptations	127:152	Plants have evolved a multitude of adaptations to survive extreme conditions.					
30980422	0	22	from	Dynamics	0:7	arg1	responses					70:78	the drought responses	58:78	the drought responses of succulent Aloe species	58:104	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	5	23	theme	content	727:733	arg1	quantification					703:716	quantification	703:716	quantification of water content	703:733	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	5	23	theme	content	727:733	arg1	combination					663:673	a combination	661:673	a combination of histological microscopy	661:700	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	5	23	theme	content	727:733	arg1	profiling					773:781	comprehensive microarray polymer profiling	740:781	comprehensive microarray polymer profiling	740:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	5	24	theme	drought	591:597	arg1	response					599:606	the drought response	587:606	the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling	587:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	0	25	theme	Aloe	93:96	arg1	species					98:104	succulent Aloe species	83:104	succulent Aloe species	83:104	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	4	26	theme	cell	460:463	arg1	wall					465:468	the cell wall	456:468	the cell wall	456:468	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	7	27	theme	cell	948:951	arg1	walls					953:957	the hydrenchyma cell walls	932:957	the hydrenchyma cell walls	932:957	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	3	28	theme	wall	350:353	arg1	polysaccharides					355:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	28	theme	wall	350:353	arg1	components					385:394	important components	375:394	important components of water storage in hydrenchyma cells	375:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	0	29	theme	intracellular	12:24	arg1	mannan					26:31	intracellular mannan	12:31	intracellular mannan	12:31	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	3	30	theme	water	399:403	arg1	storage					405:411	water storage	399:411	water storage in hydrenchyma cells	399:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	4	31	theme	succulent	542:550	arg1	plants					552:557	succulent plants	542:557	succulent plants	542:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	6	32	theme	dynamics	868:875	arg1	mode					820:823	a previously unreported mode	796:823	a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage	796:903	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	8	33	dep	folding	1358:1364	arg1	elaborate					1338:1346	elaborate	1338:1346	elaborate	1338:1346	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	0	34	theme	wall	42:45	arg1	folding					47:53	cell wall folding	37:53	cell wall folding	37:53	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	9	35	theme	cell	1406:1409	arg1	composition					1431:1441	cell wall polysaccharide composition	1406:1441	cell wall polysaccharide composition	1406:1441	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	6	36	theme	wall	852:855	arg1	dynamics					868:875	cell wall structural dynamics	847:875	cell wall structural dynamics	847:875	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	2	37	theme	specialized	305:315	arg1	tissue					317:322	a specialized tissue	303:322	a specialized tissue	303:322	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	7	38	theme	hydrenchyma	936:946	arg1	walls					953:957	the hydrenchyma cell walls	932:957	the hydrenchyma cell walls	932:957	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	9	39	theme	polysaccharide	1416:1429	arg1	composition					1431:1441	cell wall polysaccharide composition	1406:1441	cell wall polysaccharide composition	1406:1441	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	8	40	theme	in	1149:1150	arg1	distribution					1157:1168	The in situ distribution	1145:1168	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage	1145:1246	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	41	theme	Asphodelaceae	632:644	arg1	species					647:653	leaf-succulent Aloe (Asphodelaceae) species	611:653	leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling	611:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	7	42	theme	Microscopical	906:918	arg1	analysis					920:927	Microscopical analysis	906:927	Microscopical analysis of the hydrenchyma cell walls	906:957	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	9	43	theme	important	1452:1460	arg1	role					1462:1465	an important role	1449:1465	an important role	1449:1465	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	8	44	theme	mannans	1173:1179	arg1	distribution					1157:1168	The in situ distribution	1145:1168	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage	1145:1246	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	8	44	theme	mannans	1173:1179	arg1	upregulation					1262:1273	apparent upregulation	1253:1273	apparent upregulation of pectins, imparting flexibility to the cell wall,	1253:1325	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	45	theme	Aloe	626:629	arg1	species					647:653	leaf-succulent Aloe (Asphodelaceae) species	611:653	leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling	611:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	6	46	theme	structural	857:866	arg1	dynamics					868:875	cell wall structural dynamics	847:875	cell wall structural dynamics	847:875	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	5	47	theme	species	647:653	arg1	response					599:606	the drought response	587:606	the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling	587:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	8	48	theme	distinct	1184:1191	arg1	compartments					1207:1218	distinct intracellular compartments	1184:1218	distinct intracellular compartments	1184:1218	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	8	49	from	upregulation	1262:1273	arg1	compartments					1207:1218	distinct intracellular compartments	1184:1218	distinct intracellular compartments	1184:1218	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	9	50	theme	drought	1488:1494	arg1	response					1496:1503	drought response	1488:1503	drought response	1488:1503	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	7	51	theme	homogalacturonan	1111:1126	arg1	role					1103:1106	the possible role	1090:1106	the possible role of homogalacturonan in this process	1090:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	7	51	theme	homogalacturonan	1111:1126	arg1	remobilization					1055:1068	the remobilization	1051:1068	the remobilization of stored water	1051:1084	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	1	52	theme	extreme	165:171	arg1	conditions					173:182	extreme conditions	165:182	extreme conditions	165:182	Plants have evolved a multitude of adaptations to survive extreme conditions.					
30980422	4	53	from	role	448:451	arg1	relation					508:515	relation	508:515	relation to drought resistance of succulent plants	508:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	7	54	from	remobilization	1055:1068	arg1	process					1136:1142	this process	1131:1142	this process	1131:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	5	55	theme	water	721:725	arg1	content					727:733	water content	721:733	water content	721:733	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	7	56	theme	water	1080:1084	arg1	role					1103:1106	the possible role	1090:1106	the possible role of homogalacturonan in this process	1090:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	7	56	theme	water	1080:1084	arg1	remobilization					1055:1068	the remobilization	1051:1068	the remobilization of stored water	1051:1084	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	8	57	theme	apparent	1253:1260	arg1	upregulation					1262:1273	apparent upregulation	1253:1273	apparent upregulation of pectins, imparting flexibility to the cell wall,	1253:1325	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	58	theme	microscopy	691:700	arg1	quantification					703:716	quantification	703:716	quantification of water content	703:733	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	5	58	theme	microscopy	691:700	arg1	combination					663:673	a combination	661:673	a combination of histological microscopy	661:700	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	5	58	theme	microscopy	691:700	arg1	profiling					773:781	comprehensive microarray polymer profiling	740:781	comprehensive microarray polymer profiling	740:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	8	59	from	compartments	1207:1218	arg1	distribution					1157:1168	The in situ distribution	1145:1168	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage	1145:1246	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	8	59	from	compartments	1207:1218	arg1	upregulation					1262:1273	apparent upregulation	1253:1273	apparent upregulation of pectins, imparting flexibility to the cell wall,	1253:1325	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	7	60	theme	indicative	1000:1009	arg1	patterns					991:998	highly regular folding patterns	968:998	highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	968:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	5	61	theme	comprehensive	740:752	arg1	profiling					773:781	comprehensive microarray polymer profiling	740:781	comprehensive microarray polymer profiling	740:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	2	62	theme	dry	245:247	arg1	environments					249:260	periodically dry environments	232:260	periodically dry environments	232:260	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	7	63	theme	wall	1033:1036	arg1	mechanics					1038:1046	predetermined cell wall mechanics	1014:1046	predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	1014:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	8	64	theme	cell	1348:1351	arg1	folding					1358:1364	elaborate cell wall folding	1338:1364	elaborate cell wall folding	1338:1364	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	5	65	theme	polymer	765:771	arg1	profiling					773:781	comprehensive microarray polymer profiling	740:781	comprehensive microarray polymer profiling	740:781	We investigate the drought response of leaf-succulent Aloe (Asphodelaceae) species using a combination of histological microscopy, quantification of water content, and comprehensive microarray polymer profiling.					
30980422	7	66	theme	predetermined	1014:1026	arg1	mechanics					1038:1046	predetermined cell wall mechanics	1014:1046	predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	1014:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	4	67	theme	composition	493:503	arg1	unknown					563:569	unknown	563:569	unknown	563:569	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	4	67	theme	composition	493:503	arg1	role					448:451	the role	444:451	the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants	444:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	2	68	theme	Succulent	185:193	arg1	plants					195:200	Succulent plants	185:200	Succulent plants	185:200	Succulent plants have the capacity to tolerate periodically dry environments, due to their ability to retain water in a specialized tissue, termed hydrenchyma.					
30980422	0	69	theme	succulent	83:91	arg1	species					98:104	succulent Aloe species	83:104	succulent Aloe species	83:104	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	3	70	from	storage	405:411	arg1	cells					428:432	hydrenchyma cells	416:432	hydrenchyma cells	416:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	7	71	theme	regular	975:981	arg1	patterns					991:998	highly regular folding patterns	968:998	highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	968:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	3	72	theme	Cell	345:348	arg1	polysaccharides					355:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	72	theme	Cell	345:348	arg1	components					385:394	important components	375:394	important components of water storage in hydrenchyma cells	375:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	8	73	theme	drought	1373:1379	arg1	stress					1381:1386	drought stress	1373:1386	drought stress	1373:1386	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	0	74	theme	species	98:104	arg1	responses					70:78	the drought responses	58:78	the drought responses of succulent Aloe species	58:104	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	7	75	theme	folding	983:989	arg1	patterns					991:998	highly regular folding patterns	968:998	highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process	968:1142	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	4	76	theme	wall	465:468	arg1	unknown					563:569	unknown	563:569	unknown	563:569	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	4	76	theme	wall	465:468	arg1	role					448:451	the role	444:451	the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants	444:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	3	77	theme	important	375:383	arg1	polysaccharides					355:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	77	theme	important	375:383	arg1	components					385:394	important components	375:394	important components of water storage in hydrenchyma cells	375:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	78	theme	hydrenchyma	416:426	arg1	cells					428:432	hydrenchyma cells	416:432	hydrenchyma cells	416:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	0	79	theme	mannan	26:31	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.	0:105	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	7	80	theme	walls	953:957	arg1	analysis					920:927	Microscopical analysis	906:927	Microscopical analysis of the hydrenchyma cell walls	906:957	Microscopical analysis of the hydrenchyma cell walls revealed highly regular folding patterns indicative of predetermined cell wall mechanics in the remobilization of stored water and the possible role of homogalacturonan in this process.					
30980422	4	81	theme	plants	552:557	arg1	resistance					528:537	drought resistance	520:537	drought resistance of succulent plants	520:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	0	82	theme	cell	37:40	arg1	folding					47:53	cell wall folding	37:53	cell wall folding	37:53	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	8	83	from	mannans	1173:1179	arg1	compartments					1207:1218	distinct intracellular compartments	1184:1218	distinct intracellular compartments	1184:1218	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	3	84	theme	storage	405:411	arg1	polysaccharides					355:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	84	theme	storage	405:411	arg1	components					385:394	important components	375:394	important components of water storage in hydrenchyma cells	375:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	0	85	theme	folding	47:53	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.	0:105	Dynamics of intracellular mannan and cell wall folding in the drought responses of succulent Aloe species.					
30980422	8	86	from	distribution	1157:1168	arg1	compartments					1207:1218	distinct intracellular compartments	1184:1218	distinct intracellular compartments	1184:1218	The in situ distribution of mannans in distinct intracellular compartments during drought, for storage, and apparent upregulation of pectins, imparting flexibility to the cell wall, facilitate elaborate cell wall folding during drought stress.					
30980422	4	87	theme	drought	520:526	arg1	resistance					528:537	drought resistance	520:537	drought resistance of succulent plants	520:557	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	9	88	theme	wall	1411:1414	arg1	composition					1431:1441	cell wall polysaccharide composition	1406:1441	cell wall polysaccharide composition	1406:1441	We conclude that cell wall polysaccharide composition plays an important role in water storage and drought response in Aloe.					
30980422	6	89	theme	cell	847:850	arg1	dynamics					868:875	cell wall structural dynamics	847:875	cell wall structural dynamics	847:875	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	4	90	theme	polysaccharide	478:491	arg1	composition					493:503	its polysaccharide composition	474:503	its polysaccharide composition	474:503	However, the role of the cell wall and its polysaccharide composition in relation to drought resistance of succulent plants are unknown.					
30980422	6	91	theme	polysaccharide	828:841	arg1	mode					820:823	a previously unreported mode	796:823	a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage	796:903	We observed a previously unreported mode of polysaccharide and cell wall structural dynamics triggered by water shortage.					
30980422	3	92	from	cells	428:432	arg1	polysaccharides					355:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides	345:369	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
30980422	3	92	from	cells	428:432	arg1	components					385:394	important components	375:394	important components of water storage in hydrenchyma cells	375:432	Cell wall polysaccharides are important components of water storage in hydrenchyma cells.					
31736074	16	0	theme	systems	2367:2373	arg1	properties					2346:2355	thermal, pasting, and rheological properties	2312:2355	thermal, pasting, and rheological properties of starch systems	2312:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	16	1	dep	hydrocolloid	2273:2284	arg1	the					2269:2271	the	2269:2271	the	2269:2271	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	9	2	theme	infrared	1366:1373	arg1	spectra					1382:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra revealed an increase in hydrogen bonding by increasing xanthan and sucrose.					
31736074	6	3	theme	starch	1108:1113	arg1	properties					1094:1103	the thermal and pasting properties	1070:1103	the thermal and pasting properties of starch	1070:1113	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	10	4	theme	hydrogen	1489:1496	arg1	bonds					1498:1502	hydrogen bonds	1489:1502	hydrogen bonds	1489:1502	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	11	5	theme	PRACTICAL	1597:1605	arg1	APPLICATIONS					1607:1618	PRACTICAL APPLICATIONS	1597:1618	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.	1597:1785	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	16	6	theme	addition	2300:2307	arg1	effects					2258:2264	the effects	2254:2264	the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems	2254:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	3	7	theme	simultaneous	472:483	arg1	addition					485:492	simultaneous addition	472:492	simultaneous addition of sucrose and xanthan	472:515	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	9	8	theme	FTIR	1376:1379	arg1	spectra					1382:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra revealed an increase in hydrogen bonding by increasing xanthan and sucrose.					
31736074	4	9	theme	sucrose	620:626	arg1	effects					628:634	sucrose effects	620:634	sucrose effects	620:634	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	16	10	theme	thermal	2312:2318	arg1	properties					2346:2355	thermal, pasting, and rheological properties	2312:2355	thermal, pasting, and rheological properties of starch systems	2312:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	2	11	theme	main	286:289	arg1	effects					307:313	the main and interactive effects	282:313	the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch	282:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	1	12	theme	rheological	155:165	arg1	pasting					142:148	pasting	142:148	pasting	142:148	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	12	theme	rheological	155:165	arg1	characteristics					214:228	the key characteristics	206:228	the key characteristics for its processing	206:247	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	12	theme	rheological	155:165	arg1	Thermal					133:139	Thermal	133:139	Thermal	133:139	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	12	theme	rheological	155:165	arg1	properties					167:176	rheological properties	155:176	rheological properties	155:176	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	11	13	from	countries	1705:1713	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	13	from	countries	1705:1713	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	8	14	theme	Regression	1198:1207	arg1	models					1209:1214	Regression models	1198:1214	Regression models	1198:1214	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	13	15	from	aspects	1962:1968	arg1	processing					1977:1986	its processing	1973:1986	its processing	1973:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	0	16	theme	rheological	96:106	arg1	behavior					108:115	the thermal, pasting, and rheological behavior	70:115	the thermal, pasting, and rheological behavior of corn starch	70:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	12	17	theme	great	1851:1855	arg1	importance					1857:1866	great importance	1851:1866	great importance	1851:1866	Therefore, the production of these kinds of food products is of great importance.					
31736074	1	18	theme	systems	194:200	arg1	pasting					142:148	pasting	142:148	pasting	142:148	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	18	theme	systems	194:200	arg1	characteristics					214:228	the key characteristics	206:228	the key characteristics for its processing	206:247	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	18	theme	systems	194:200	arg1	Thermal					133:139	Thermal	133:139	Thermal	133:139	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	18	theme	systems	194:200	arg1	properties					167:176	rheological properties	155:176	rheological properties	155:176	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	11	19	from	foods	1681:1685	arg1	countries					1705:1713	the developing countries	1690:1713	the developing countries	1690:1713	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	0	20	from	effects	59:65	arg1	behavior					108:115	the thermal, pasting, and rheological behavior	70:115	the thermal, pasting, and rheological behavior of corn starch	70:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	14	21	theme	ingredients	2058:2068	arg1	presence					2040:2047	the presence	2036:2047	the presence of other ingredients such as hydrocolloids and sweeteners	2036:2105	These properties show differences according to the presence of other ingredients such as hydrocolloids and sweeteners.					
31736074	11	22	theme	energy	1746:1751	arg1	deal					1738:1741	a great deal	1730:1741	a great deal of energy	1730:1751	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	0	23	theme	starch	125:130	arg1	behavior					108:115	the thermal, pasting, and rheological behavior	70:115	the thermal, pasting, and rheological behavior of corn starch	70:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	3	24	theme	processing	525:534	arg1	properties					536:545	some processing properties	520:545	some processing properties of starch-based systems	520:569	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	5	25	theme	enthalpy	850:857	arg1	ΔH					867:868	ΔH	867:868	ΔH	867:868	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	5	25	theme	enthalpy	850:857	arg1	change					859:864	the enthalpy change	846:864	the enthalpy change (ΔH) of the starch gelatinization	846:898	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	16	26	theme	sauces	2465:2470	arg1	production					2451:2460	the production	2447:2460	the production of sauces, baby foods, bread, confectionery, etc	2447:2509	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	8	27	theme	properties	1258:1267	arg1	prediction					1229:1238	prediction	1229:1238	prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88)	1229:1341	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	11	28	theme	foods	1681:1685	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	28	theme	foods	1681:1685	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	7	29	theme	interaction	1167:1177	arg1	effects					1179:1185	interaction effects	1167:1185	interaction effects	1167:1185	These were ascribed to the first- and second-order interaction effects (p < .05).					
31736074	3	30	theme	systems	563:569	arg1	properties					536:545	some processing properties	520:545	some processing properties of starch-based systems	520:569	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	5	31	theme	transition	818:827	arg1	temperatures					829:840	the transition temperatures	814:840	the transition temperatures	814:840	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	16	32	theme	baby	2473:2476	arg1	sauces					2465:2470	sauces	2465:2470	sauces	2465:2470	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	16	32	theme	baby	2473:2476	arg1	foods					2478:2482	baby foods	2473:2482	baby foods	2473:2482	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	11	33	from	kind	1673:1676	arg1	diet					1781:1784	a healthy diet	1771:1784	a healthy diet	1771:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	33	from	kind	1673:1676	arg1	countries					1705:1713	the developing countries	1690:1713	the developing countries	1690:1713	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	14	34	theme	other	2052:2056	arg1	sweeteners					2096:2105	sweeteners	2096:2105	sweeteners	2096:2105	These properties show differences according to the presence of other ingredients such as hydrocolloids and sweeteners.					
31736074	14	34	theme	other	2052:2056	arg1	ingredients					2058:2068	other ingredients	2052:2068	other ingredients such as hydrocolloids and sweeteners	2052:2105	These properties show differences according to the presence of other ingredients such as hydrocolloids and sweeteners.					
31736074	14	34	theme	other	2052:2056	arg1	hydrocolloids					2078:2090	hydrocolloids	2078:2090	hydrocolloids	2078:2090	These properties show differences according to the presence of other ingredients such as hydrocolloids and sweeteners.					
31736074	15	35	from	sweetener	2176:2184	arg1	foods					2202:2206	starch-based foods	2189:2206	starch-based foods	2189:2206	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	4	36	theme	considered	698:707	arg1	properties					709:718	all considered properties	694:718	all considered properties	694:718	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	13	37	theme	imperative	1951:1960	arg1	properties					1914:1923	The gelatinization, pasting, and rheological properties	1869:1923	The gelatinization, pasting, and rheological properties of starch-based foods	1869:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	13	37	theme	imperative	1951:1960	arg1	aspects					1962:1968	imperative aspects	1951:1968	imperative aspects in its processing	1951:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	12	38	theme	products	1836:1843	arg1	kinds					1822:1826	these kinds	1816:1826	these kinds of food products	1816:1843	Therefore, the production of these kinds of food products is of great importance.					
31736074	8	39	from	prediction	1229:1238	arg1	range					1305:1309	the range	1301:1309	the range of selected factors (R2 > 0.88)	1301:1341	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	5	40	theme	gelatinization	885:898	arg1	temperatures					829:840	the transition temperatures	814:840	the transition temperatures	814:840	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	5	40	theme	gelatinization	885:898	arg1	ΔH					867:868	ΔH	867:868	ΔH	867:868	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	5	40	theme	gelatinization	885:898	arg1	change					859:864	the enthalpy change	846:864	the enthalpy change (ΔH) of the starch gelatinization	846:898	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	15	41	theme	optimal	2216:2222	arg1	condition					2235:2243	optimal processing condition	2216:2243	optimal processing condition	2216:2243	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	6	42	theme	synergistic	1013:1023	arg1	effects					1025:1031	the synergistic effects	1009:1031	the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch	1009:1113	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	4	43	theme	significant	674:684	arg1	role					686:689	a significant role	672:689	a significant role in all considered properties of corn starch	672:733	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	10	44	theme	starch	1582:1587	arg1	pastes					1589:1594	the starch pastes	1578:1594	the starch pastes	1578:1594	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	2	45	theme	starch	396:401	arg1	behavior					379:386	the thermal, pasting, and rheological behavior	341:386	the thermal, pasting, and rheological behavior of corn starch	341:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	7	46	dep	first-	1143:1148	arg1	effects					1179:1185	interaction effects	1167:1185	interaction effects	1167:1185	These were ascribed to the first- and second-order interaction effects (p < .05).					
31736074	7	46	dep	first-	1143:1148	arg1	the					1139:1141	the	1139:1141	the	1139:1141	These were ascribed to the first- and second-order interaction effects (p < .05).					
31736074	13	47	theme	pasting	1889:1895	arg1	properties					1914:1923	The gelatinization, pasting, and rheological properties	1869:1923	The gelatinization, pasting, and rheological properties of starch-based foods	1869:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	13	47	theme	pasting	1889:1895	arg1	aspects					1962:1968	imperative aspects	1951:1968	imperative aspects in its processing	1951:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	8	48	theme	factors	1323:1329	arg1	range					1305:1309	the range	1301:1309	the range of selected factors (R2 > 0.88)	1301:1341	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	4	49	theme	behavior	751:758	arg1	p < .05					767:773	p < .05	767:773	p < .05	767:773	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	4	49	theme	behavior	751:758	arg1	index					760:764	the flow behavior index	742:764	the flow behavior index (p < .05)	742:774	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	0	50	theme	simultaneous	14:25	arg1	addition					27:34	simultaneous addition	14:34	simultaneous addition of sucrose and xanthan	14:57	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	16	51	theme	optimal	2416:2422	arg1	conditions					2432:2441	optimal process conditions	2416:2441	optimal process conditions	2416:2441	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	6	52	theme	analysis	983:990	arg1	results					992:998	Statistical analysis results	971:998	Statistical analysis results	971:998	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	2	53	theme	rheological	367:377	arg1	behavior					379:386	the thermal, pasting, and rheological behavior	341:386	the thermal, pasting, and rheological behavior of corn starch	341:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	8	54	theme	R2	1332:1333	arg1	> 0.88					1335:1340	R2 > 0.88	1332:1340	R2 > 0.88	1332:1340	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	8	54	theme	R2	1332:1333	arg1	factors					1323:1329	selected factors	1314:1329	selected factors (R2 > 0.88)	1314:1341	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	3	55	theme	central	406:412	arg1	CCD					432:434	CCD	432:434	CCD	432:434	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	3	55	theme	central	406:412	arg1	design					424:429	A central composite design	404:429	A central composite design (CCD)	404:435	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	15	56	theme	common	2169:2174	arg1	sweetener					2176:2184	a common sweetener	2167:2184	a common sweetener in starch-based foods	2167:2206	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	9	57	from	increase	1402:1409	arg1	bonding					1423:1429	hydrogen bonding	1414:1429	hydrogen bonding	1414:1429	The Fourier-transform infrared (FTIR) spectra revealed an increase in hydrogen bonding by increasing xanthan and sucrose.					
31736074	3	58	used	used	441:444	arg2	CCD					432:434	CCD	432:434	CCD	432:434	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	3	58	used	used	441:444	arg2	design					424:429	A central composite design	404:429	A central composite design (CCD)	404:435	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	13	59	theme	starch-based	1928:1939	arg1	foods					1941:1945	starch-based foods	1928:1945	starch-based foods	1928:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	2	60	theme	sucrose	318:324	arg1	effects					307:313	the main and interactive effects	282:313	the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch	282:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	4	61	theme	starch	728:733	arg1	role					686:689	a significant role	672:689	a significant role in all considered properties of corn starch	672:733	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	15	62	theme	xanthan	2130:2136	arg1	addition					2118:2125	the addition	2114:2125	the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods	2114:2206	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	11	63	theme	consumed	1664:1671	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	63	theme	consumed	1664:1671	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	16	64	theme	rheological	2334:2344	arg1	properties					2346:2355	thermal, pasting, and rheological properties	2312:2355	thermal, pasting, and rheological properties of starch systems	2312:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	1	65	theme	key	210:212	arg1	pasting					142:148	pasting	142:148	pasting	142:148	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	65	theme	key	210:212	arg1	characteristics					214:228	the key characteristics	206:228	the key characteristics for its processing	206:247	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	65	theme	key	210:212	arg1	Thermal					133:139	Thermal	133:139	Thermal	133:139	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	1	65	theme	key	210:212	arg1	properties					167:176	rheological properties	155:176	rheological properties	155:176	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	5	66	theme	pasting	926:932	arg1	properties					934:943	all pasting properties	922:943	all pasting properties	922:943	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	2	67	theme	xanthan	330:336	arg1	effects					307:313	the main and interactive effects	282:313	the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch	282:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	15	68	theme	processing	2224:2233	arg1	condition					2235:2243	optimal processing condition	2216:2243	optimal processing condition	2216:2243	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	2	69	from	effects	307:313	arg1	behavior					379:386	the thermal, pasting, and rheological behavior	341:386	the thermal, pasting, and rheological behavior of corn starch	341:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	6	70	theme	due	1033:1035	arg1	effects					1025:1031	the synergistic effects	1009:1031	the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch	1009:1113	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	10	71	theme	mixture	1512:1518	arg1	components					1520:1529	mixture components	1512:1529	mixture components	1512:1529	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	13	72	theme	gelatinization	1873:1886	arg1	properties					1914:1923	The gelatinization, pasting, and rheological properties	1869:1923	The gelatinization, pasting, and rheological properties of starch-based foods	1869:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	13	72	theme	gelatinization	1873:1886	arg1	aspects					1962:1968	imperative aspects	1951:1968	imperative aspects in its processing	1951:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	3	73	theme	addition	485:492	arg1	effect					462:467	the effect	458:467	the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems	458:569	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	9	74	theme	Fourier-transform	1348:1364	arg1	spectra					1382:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra	1344:1388	The Fourier-transform infrared (FTIR) spectra revealed an increase in hydrogen bonding by increasing xanthan and sucrose.					
31736074	11	75	from	nutrients	1758:1766	arg1	countries					1705:1713	the developing countries	1690:1713	the developing countries	1690:1713	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	10	76	theme	bonds	1498:1502	arg1	formation					1476:1484	the formation	1472:1484	the formation of hydrogen bonds between mixture components	1472:1529	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	11	77	theme	Starch-based	1621:1632	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	77	theme	Starch-based	1621:1632	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	3	78	from	effect	462:467	arg1	properties					536:545	some processing properties	520:545	some processing properties of starch-based systems	520:569	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	16	79	theme	sweetener	2290:2298	arg1	addition					2300:2307	sweetener addition	2290:2307	sweetener addition	2290:2307	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	3	80	theme	xanthan	509:515	arg1	addition					485:492	simultaneous addition	472:492	simultaneous addition of sucrose and xanthan	472:515	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	6	81	theme	pasting	1086:1092	arg1	properties					1094:1103	the thermal and pasting properties	1070:1103	the thermal and pasting properties of starch	1070:1113	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	3	82	theme	sucrose	497:503	arg1	addition					485:492	simultaneous addition	472:492	simultaneous addition of sucrose and xanthan	472:515	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	6	83	theme	thermal	1074:1080	arg1	properties					1094:1103	the thermal and pasting properties	1070:1103	the thermal and pasting properties of starch	1070:1113	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	11	84	theme	great	1732:1736	arg1	deal					1738:1741	a great deal	1730:1741	a great deal of energy	1730:1751	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	0	85	theme	corn	120:123	arg1	starch					125:130	corn starch	120:130	corn starch	120:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	1	86	theme	starch-based	181:192	arg1	systems					194:200	starch-based systems	181:200	starch-based systems	181:200	Thermal, pasting, and rheological properties of starch-based systems are the key characteristics for its processing.					
31736074	10	87	theme	considered	1553:1562	arg1	properties					1564:1573	the considered properties	1549:1573	the considered properties of the starch pastes	1549:1594	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	11	88	theme	nutrients	1758:1766	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	88	theme	nutrients	1758:1766	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	9	89	theme	hydrogen	1414:1421	arg1	bonding					1423:1429	hydrogen bonding	1414:1429	hydrogen bonding	1414:1429	The Fourier-transform infrared (FTIR) spectra revealed an increase in hydrogen bonding by increasing xanthan and sucrose.					
31736074	8	90	theme	mixtures	1289:1296	arg1	properties					1258:1267	all considered properties	1243:1267	all considered properties of the starch-based mixtures	1243:1296	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	11	91	theme	developing	1694:1703	arg1	countries					1705:1713	the developing countries	1690:1713	the developing countries	1690:1713	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	3	92	theme	starch-based	550:561	arg1	systems					563:569	starch-based systems	550:569	starch-based systems	550:569	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	8	93	theme	considered	1247:1256	arg1	properties					1258:1267	all considered properties	1243:1267	all considered properties of the starch-based mixtures	1243:1296	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	0	94	theme	thermal	74:80	arg1	behavior					108:115	the thermal, pasting, and rheological behavior	70:115	the thermal, pasting, and rheological behavior of corn starch	70:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	12	95	theme	food	1831:1834	arg1	products					1836:1843	food products	1831:1843	food products	1831:1843	Therefore, the production of these kinds of food products is of great importance.					
31736074	11	96	theme	healthy	1773:1779	arg1	diet					1781:1784	a healthy diet	1771:1784	a healthy diet	1771:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	16	97	theme	hydrocolloid	2273:2284	arg1	effects					2258:2264	the effects	2254:2264	the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems	2254:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	0	98	theme	pasting	83:89	arg1	behavior					108:115	the thermal, pasting, and rheological behavior	70:115	the thermal, pasting, and rheological behavior of corn starch	70:130	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	5	99	theme	starch	878:883	arg1	gelatinization					885:898	the starch gelatinization	874:898	the starch gelatinization	874:898	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	8	100	theme	starch-based	1276:1287	arg1	mixtures					1289:1296	the starch-based mixtures	1272:1296	the starch-based mixtures	1272:1296	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	16	101	dep	help	2376:2379	arg1	Knowing					2246:2252	Knowing	2246:2252	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems	2246:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	10	102	theme	pastes	1589:1594	arg1	properties					1564:1573	the considered properties	1549:1573	the considered properties of the starch pastes	1549:1594	Since the formation of hydrogen bonds between mixture components could be affected the considered properties of the starch pastes.					
31736074	12	103	theme	kinds	1822:1826	arg1	production					1802:1811	the production	1798:1811	the production of these kinds of food products	1798:1843	Therefore, the production of these kinds of food products is of great importance.					
31736074	4	104	from	role	686:689	arg1	properties					709:718	all considered properties	694:718	all considered properties	694:718	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	16	105	from	effects	2258:2264	arg1	properties					2346:2355	thermal, pasting, and rheological properties	2312:2355	thermal, pasting, and rheological properties of starch systems	2312:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	2	106	theme	thermal	345:351	arg1	behavior					379:386	the thermal, pasting, and rheological behavior	341:386	the thermal, pasting, and rheological behavior of corn starch	341:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	0	107	theme	addition	27:34	arg1	Assessment					0:9	Assessment	0:9	Assessment of simultaneous addition of sucrose and xanthan	0:57	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	2	108	theme	corn	391:394	arg1	starch					396:401	corn starch	391:401	corn starch	391:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	8	109	theme	selected	1314:1321	arg1	> 0.88					1335:1340	R2 > 0.88	1332:1340	R2 > 0.88	1332:1340	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	8	109	theme	selected	1314:1321	arg1	factors					1323:1329	selected factors	1314:1329	selected factors (R2 > 0.88)	1314:1341	Regression models proposed for prediction of all considered properties of the starch-based mixtures in the range of selected factors (R2 > 0.88).					
31736074	0	110	theme	sucrose	39:45	arg1	addition					27:34	simultaneous addition	14:34	simultaneous addition of sucrose and xanthan	14:57	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	15	111	theme	starch-based	2189:2200	arg1	foods					2202:2206	starch-based foods	2189:2206	starch-based foods	2189:2206	Since the addition of xanthan as hydrocolloid and sugar as a common sweetener in starch-based foods, affect optimal processing condition.					
31736074	6	112	theme	Statistical	971:981	arg1	results					992:998	Statistical analysis results	971:998	Statistical analysis results	971:998	Statistical analysis results revealed the synergistic effects due to adding xanthan and sucrose on the thermal and pasting properties of starch.					
31736074	4	113	theme	flow	746:749	arg1	p < .05					767:773	p < .05	767:773	p < .05	767:773	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	4	113	theme	flow	746:749	arg1	index					760:764	the flow behavior index	742:764	the flow behavior index (p < .05)	742:774	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	16	114	theme	pasting	2321:2327	arg1	properties					2346:2355	thermal, pasting, and rheological properties	2312:2355	thermal, pasting, and rheological properties of starch systems	2312:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	3	115	theme	composite	414:422	arg1	CCD					432:434	CCD	432:434	CCD	432:434	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	3	115	theme	composite	414:422	arg1	design					424:429	A central composite design	404:429	A central composite design (CCD)	404:435	A central composite design (CCD) was used to evaluate the effect of simultaneous addition of sucrose and xanthan on some processing properties of starch-based systems.					
31736074	0	116	theme	xanthan	51:57	arg1	addition					27:34	simultaneous addition	14:34	simultaneous addition of sucrose and xanthan	14:57	Assessment of simultaneous addition of sucrose and xanthan effects on the thermal, pasting, and rheological behavior of corn starch.					
31736074	11	117	dep	APPLICATIONS	1607:1618	arg1	foods					1634:1638	Starch-based foods	1621:1638	Starch-based foods	1621:1638	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	11	117	dep	APPLICATIONS	1607:1618	arg1	kind					1673:1676	the most frequently consumed kind	1644:1676	the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet	1644:1784	PRACTICAL APPLICATIONS: Starch-based foods are the most frequently consumed kind of foods in the developing countries, which provide a great deal of energy, and nutrients in a healthy diet.					
31736074	16	118	theme	process	2424:2430	arg1	conditions					2432:2441	optimal process conditions	2416:2441	optimal process conditions	2416:2441	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	5	119	theme	setback	952:958	arg1	viscosity					960:968	setback viscosity	952:968	setback viscosity	952:968	Adding xanthan and sucrose increased the transition temperatures and the enthalpy change (ΔH) of the starch gelatinization, while they increased all pasting properties except setback viscosity.					
31736074	13	120	theme	foods	1941:1945	arg1	properties					1914:1923	The gelatinization, pasting, and rheological properties	1869:1923	The gelatinization, pasting, and rheological properties of starch-based foods	1869:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	13	120	theme	foods	1941:1945	arg1	aspects					1962:1968	imperative aspects	1951:1968	imperative aspects in its processing	1951:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	16	121	theme	starch	2360:2365	arg1	systems					2367:2373	starch systems	2360:2373	starch systems	2360:2373	Knowing the effects of the hydrocolloid and sweetener addition on thermal, pasting, and rheological properties of starch systems, help to improve the process and finding optimal process conditions for the production of sauces, baby foods, bread, confectionery, etc.					
31736074	4	122	theme	corn	723:726	arg1	starch					728:733	corn starch	723:733	corn starch	723:733	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	2	123	theme	interactive	295:305	arg1	effects					307:313	the main and interactive effects	282:313	the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch	282:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	4	124	dep	xanthan	608:614	arg1	the					604:606	the	604:606	the	604:606	The results showed that besides the xanthan and sucrose effects (p < .001), the interactions played a significant role in all considered properties of corn starch except the flow behavior index (p < .05).					
31736074	2	125	theme	pasting	354:360	arg1	behavior					379:386	the thermal, pasting, and rheological behavior	341:386	the thermal, pasting, and rheological behavior of corn starch	341:401	This study aimed to investigate the main and interactive effects of sucrose and xanthan on the thermal, pasting, and rheological behavior of corn starch.					
31736074	13	126	theme	rheological	1902:1912	arg1	properties					1914:1923	The gelatinization, pasting, and rheological properties	1869:1923	The gelatinization, pasting, and rheological properties of starch-based foods	1869:1945	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31736074	13	126	theme	rheological	1902:1912	arg1	aspects					1962:1968	imperative aspects	1951:1968	imperative aspects in its processing	1951:1986	The gelatinization, pasting, and rheological properties of starch-based foods are imperative aspects in its processing.					
31151623	0	0	theme	controlled	90:99	arg1	drop					110:113	instant controlled pressure drop	82:113	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	1	theme	pressure	330:337	arg1	technology					344:353	instant controlled pressure drop technology	311:353	instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	311:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	2	theme	acid	814:817	arg1	content					819:825	galacturonic acid content	801:825	galacturonic acid content	801:825	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	3	theme	sugar	853:857	arg1	composition					859:869	sugar composition	853:869	sugar composition	853:869	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	0	4	theme	instant	82:88	arg1	drop					110:113	instant controlled pressure drop	82:113	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	5	theme	drop	339:342	arg1	technology					344:353	instant controlled pressure drop technology	311:353	instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	311:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	6	theme	different	916:924	arg1	zones					933:937	different tissue zones	916:937	different tissue zones of raw carrots	916:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	amount					773:778	the amount	769:778	the amount of pectic fractions	769:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	composition					859:869	sugar composition	853:869	sugar composition	853:869	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	content					819:825	galacturonic acid content	801:825	galacturonic acid content	801:825	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	degree					828:833	degree	828:833	degree of methoxylation	828:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	linearity					875:883	linearity	875:883	linearity	875:883	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	fractions					790:798	pectic fractions	783:798	pectic fractions	783:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	CWPs					888:891	CWPs	888:891	CWPs that fractionated from different tissue zones of raw carrots	888:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	7	theme	fractions	790:798	arg1	methoxylation					838:850	methoxylation	838:850	methoxylation	838:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	4	8	theme	expanding	1219:1227	arg1	behaviors					1229:1237	the expanding behaviors	1215:1237	the expanding behaviors	1215:1237	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	0	9	theme	drop	110:113	arg1	chips					128:132	instant controlled pressure drop dried carrot chips	82:132	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	4	10	theme	textural	1076:1083	arg1	properties					1085:1094	textural properties	1076:1094	textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1076:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	1	11	theme	CWPs	427:430	arg1	characteristics					408:422	the characteristics	404:422	the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot	404:535	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	4	12	theme	relationship	1172:1183	arg1	insight					1157:1163	a mechanistic insight	1143:1163	a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1143:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	0	13	theme	pressure	101:108	arg1	drop					110:113	instant controlled pressure drop	82:113	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	4	14	theme	DIC-dried	1242:1250	arg1	fruits					1252:1257	DIC-dried fruits	1242:1257	DIC-dried fruits	1242:1257	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	15	theme	DIC-dried	1103:1111	arg1	chips					1120:1124	the DIC-dried carrot chips	1099:1124	the DIC-dried carrot chips	1099:1124	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	3	16	theme	tissue	926:931	arg1	zones					933:937	different tissue zones	916:937	different tissue zones of raw carrots	916:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	4	17	theme	expansion	1056:1064	arg1	ratio					1066:1070	the expansion ratio	1052:1070	the expansion ratio	1052:1070	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	0	18	theme	carrot	121:126	arg1	chips					128:132	instant controlled pressure drop dried carrot chips	82:132	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	4	19	theme	substantial	1005:1015	arg1	factor					1017:1022	a substantial factor	1003:1022	a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1003:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	19	theme	substantial	1005:1015	arg1	characteristics					959:973	The characteristics	955:973	The characteristics of CWPs	955:981	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	2	20	theme	expansion	563:571	arg1	volume					573:578	Larger expansion volume	556:578	Larger expansion volume	556:578	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	1	21	theme	ground	437:442	arg1	GT					452:453	GT	452:453	GT	452:453	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	21	theme	ground	437:442	arg1	tissue					444:449	ground tissue	437:449	ground tissue (GT)	437:454	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	22	attach	derived	134:140	arg2	chips					128:132	instant controlled pressure drop dried carrot chips	82:132	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	0	22	attach	derived	134:140	arg1	zone					164:167	different tissue zone	147:167	different tissue zone	147:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	0	23	theme	dried	115:119	arg1	chips					128:132	instant controlled pressure drop dried carrot chips	82:132	instant controlled pressure drop dried carrot chips derived from different tissue zone	82:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	2	24	theme	Larger	556:561	arg1	volume					573:578	Larger expansion volume	556:578	Larger expansion volume	556:578	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	4	25	theme	fruits	1252:1257	arg1	CWPs					1206:1209	CWPs	1206:1209	CWPs	1206:1209	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	25	theme	fruits	1252:1257	arg1	behaviors					1229:1237	the expanding behaviors	1215:1237	the expanding behaviors	1215:1237	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	2	26	attach	derived	613:619	arg2	chips					607:611	the carrot chips	596:611	the carrot chips	596:611	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	2	26	attach	derived	613:619	arg1	GT					626:627	GT	626:627	GT	626:627	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	1	27	theme	textural	266:273	arg1	properties					275:284	textural properties	266:284	textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	266:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	2	28	theme	carrot	600:605	arg1	chips					607:611	the carrot chips	596:611	the carrot chips	596:611	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	1	29	theme	vascular	506:513	arg1	tissue					515:520	vascular tissue	506:520	vascular tissue (VT)	506:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	29	theme	vascular	506:513	arg1	VT					523:524	VT	523:524	VT	523:524	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	30	theme	wall	24:27	arg1	polysaccharides					36:50	cell wall pectic polysaccharides	19:50	cell wall pectic polysaccharides	19:50	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	31	theme	properties	275:284	arg1	formation					253:261	the formation	249:261	the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	249:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	32	theme	carrots	946:952	arg1	zones					933:937	different tissue zones	916:937	different tissue zones of raw carrots	916:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	0	33	theme	cell	19:22	arg1	polysaccharides					36:50	cell wall pectic polysaccharides	19:50	cell wall pectic polysaccharides	19:50	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	0	34	theme	chips	128:132	arg1	properties					68:77	textural properties	59:77	textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone	59:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	3	35	theme	methoxylation	838:850	arg1	amount					773:778	the amount	769:778	the amount of pectic fractions	769:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	composition					859:869	sugar composition	853:869	sugar composition	853:869	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	content					819:825	galacturonic acid content	801:825	galacturonic acid content	801:825	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	degree					828:833	degree	828:833	degree of methoxylation	828:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	linearity					875:883	linearity	875:883	linearity	875:883	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	fractions					790:798	pectic fractions	783:798	pectic fractions	783:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	CWPs					888:891	CWPs	888:891	CWPs that fractionated from different tissue zones of raw carrots	888:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	35	theme	methoxylation	838:850	arg1	methoxylation					838:850	methoxylation	838:850	methoxylation	838:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	1	36	theme	cell	206:209	arg1	CWPs					240:243	CWPs	240:243	CWPs	240:243	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	36	theme	cell	206:209	arg1	polysaccharides					223:237	cell wall pectic polysaccharides	206:237	cell wall pectic polysaccharides (CWPs)	206:244	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	37	theme	polysaccharides	36:50	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of cell wall pectic polysaccharides	0:50	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	38	theme	carrot	289:294	arg1	chips					296:300	carrot chips	289:300	carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	289:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	39	theme	different	147:155	arg1	zone					164:167	different tissue zone	147:167	different tissue zone	147:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	40	theme	wall	211:214	arg1	CWPs					240:243	CWPs	240:243	CWPs	240:243	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	40	theme	wall	211:214	arg1	polysaccharides					223:237	cell wall pectic polysaccharides	206:237	cell wall pectic polysaccharides (CWPs)	206:244	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	41	theme	pectic	29:34	arg1	polysaccharides					36:50	cell wall pectic polysaccharides	19:50	cell wall pectic polysaccharides	19:50	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	42	theme	chips	296:300	arg1	properties					275:284	textural properties	266:284	textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	266:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	2	43	attach	derived	705:711	arg1	VT					725:726	VT	725:726	VT	725:726	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	2	43	attach	derived	705:711	arg2	chips					699:703	the chips	695:703	the chips derived from JT and VT	695:726	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	2	43	attach	derived	705:711	arg1	JT					718:719	JT	718:719	JT	718:719	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	4	44	theme	CWPs	978:981	arg1	factor					1017:1022	a substantial factor	1003:1022	a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1003:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	44	theme	CWPs	978:981	arg1	characteristics					959:973	The characteristics	955:973	The characteristics of CWPs	955:981	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	45	theme	carrot	1113:1118	arg1	chips					1120:1124	the DIC-dried carrot chips	1099:1124	the DIC-dried carrot chips	1099:1124	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	1	46	theme	pectic	216:221	arg1	CWPs					240:243	CWPs	240:243	CWPs	240:243	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	46	theme	pectic	216:221	arg1	polysaccharides					223:237	cell wall pectic polysaccharides	206:237	cell wall pectic polysaccharides (CWPs)	206:244	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	0	47	theme	textural	59:66	arg1	properties					68:77	textural properties	59:77	textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone	59:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	48	dep	technology	344:353	arg1	French					356:361	French	356:361	French	356:361	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	49	theme	CWPs	888:891	arg1	amount					773:778	the amount	769:778	the amount of pectic fractions	769:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	composition					859:869	sugar composition	853:869	sugar composition	853:869	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	content					819:825	galacturonic acid content	801:825	galacturonic acid content	801:825	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	degree					828:833	degree	828:833	degree of methoxylation	828:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	linearity					875:883	linearity	875:883	linearity	875:883	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	fractions					790:798	pectic fractions	783:798	pectic fractions	783:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	CWPs					888:891	CWPs	888:891	CWPs that fractionated from different tissue zones of raw carrots	888:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	3	49	theme	CWPs	888:891	arg1	methoxylation					838:850	methoxylation	838:850	methoxylation	838:850	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	0	50	theme	tissue	157:162	arg1	zone					164:167	different tissue zone	147:167	different tissue zone	147:167	Characteristics of cell wall pectic polysaccharides affect textural properties of instant controlled pressure drop dried carrot chips derived from different tissue zone.					
31151623	1	51	from	tissue	515:520	arg1	CWPs					427:430	CWPs	427:430	CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT)	427:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	51	from	tissue	515:520	arg1	characteristics					408:422	the characteristics	404:422	the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot	404:535	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	52	theme	instant	311:317	arg1	technology					344:353	instant controlled pressure drop technology	311:353	instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	311:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	4	53	from	variation	1193:1201	arg1	CWPs					1206:1209	CWPs	1206:1209	CWPs	1206:1209	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	53	from	variation	1193:1201	arg1	behaviors					1229:1237	the expanding behaviors	1215:1237	the expanding behaviors	1215:1237	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	1	54	theme	ground	469:474	arg1	tissue					515:520	vascular tissue	506:520	vascular tissue (VT)	506:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	54	theme	ground	469:474	arg1	GT					452:453	GT	452:453	GT	452:453	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	54	theme	ground	469:474	arg1	junction					457:464	junction	457:464	junction of ground and vascular tissue (JT)	457:499	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	54	theme	ground	469:474	arg1	tissue					444:449	ground tissue	437:449	ground tissue (GT)	437:454	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	54	theme	ground	469:474	arg1	VT					523:524	VT	523:524	VT	523:524	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	55	from	tissue	444:449	arg1	CWPs					427:430	CWPs	427:430	CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT)	427:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	55	from	tissue	444:449	arg1	characteristics					408:422	the characteristics	404:422	the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot	404:535	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	56	theme	pectic	783:788	arg1	fractions					790:798	pectic fractions	783:798	pectic fractions	783:798	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	1	57	theme	polysaccharides	223:237	arg1	role					198:201	the role	194:201	the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	194:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	4	58	theme	chips	1120:1124	arg1	ratio					1066:1070	the expansion ratio	1052:1070	the expansion ratio	1052:1070	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	58	theme	chips	1120:1124	arg1	properties					1085:1094	textural properties	1076:1094	textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1076:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	2	59	theme	textural	662:669	arg1	qualities					671:679	superior textural qualities	653:679	superior textural qualities	653:679	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	2	60	theme	superior	653:660	arg1	qualities					671:679	superior textural qualities	653:679	superior textural qualities	653:679	Larger expansion volume was obtained in the carrot chips derived from GT, which accompanied with superior textural qualities compared with the chips derived from JT and VT.					
31151623	1	61	theme	carrot	530:535	arg1	characteristics					408:422	the characteristics	404:422	the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot	404:535	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	62	theme	Remarkable	729:738	arg1	differences					740:750	Remarkable differences	729:750	Remarkable differences	729:750	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	1	63	from	junction	457:464	arg1	CWPs					427:430	CWPs	427:430	CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT)	427:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	63	from	junction	457:464	arg1	characteristics					408:422	the characteristics	404:422	the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot	404:535	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	64	theme	raw	942:944	arg1	carrots					946:952	raw carrots	942:952	raw carrots	942:952	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31151623	1	65	theme	vascular	480:487	arg1	JT					497:498	JT	497:498	JT	497:498	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	65	theme	vascular	480:487	arg1	tissue					489:494	vascular tissue	480:494	vascular tissue (JT)	480:499	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	66	from	role	198:201	arg1	formation					253:261	the formation	249:261	the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	249:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	4	67	theme	vegetables	1263:1272	arg1	CWPs					1206:1209	CWPs	1206:1209	CWPs	1206:1209	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	4	67	theme	vegetables	1263:1272	arg1	behaviors					1229:1237	the expanding behaviors	1215:1237	the expanding behaviors	1215:1237	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	1	68	from	characteristics	408:422	arg1	tissue					515:520	vascular tissue	506:520	vascular tissue (VT)	506:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	68	from	characteristics	408:422	arg1	GT					452:453	GT	452:453	GT	452:453	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	68	from	characteristics	408:422	arg1	junction					457:464	junction	457:464	junction of ground and vascular tissue (JT)	457:499	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	68	from	characteristics	408:422	arg1	tissue					444:449	ground tissue	437:449	ground tissue (GT)	437:454	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	68	from	characteristics	408:422	arg1	VT					523:524	VT	523:524	VT	523:524	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	69	theme	controlled	319:328	arg1	technology					344:353	instant controlled pressure drop technology	311:353	instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC)	311:401	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	4	70	theme	mechanistic	1145:1155	arg1	insight					1157:1163	a mechanistic insight	1143:1163	a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables	1143:1272	The characteristics of CWPs was confirmed to be a substantial factor that significantly affected the expansion ratio and textural properties of the DIC-dried carrot chips, which providing a mechanistic insight of the relationship between variation in CWPs and the expanding behaviors of DIC-dried fruits and vegetables.					
31151623	1	71	theme	tissue	489:494	arg1	tissue					515:520	vascular tissue	506:520	vascular tissue (VT)	506:525	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	71	theme	tissue	489:494	arg1	GT					452:453	GT	452:453	GT	452:453	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	71	theme	tissue	489:494	arg1	junction					457:464	junction	457:464	junction of ground and vascular tissue (JT)	457:499	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	71	theme	tissue	489:494	arg1	tissue					444:449	ground tissue	437:449	ground tissue (GT)	437:454	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	1	71	theme	tissue	489:494	arg1	VT					523:524	VT	523:524	VT	523:524	To better understanding the role of cell wall pectic polysaccharides (CWPs) on the formation of textural properties of carrot chips dried by instant controlled pressure drop technology (French for Détente Instantanée Contrôlée, DIC), the characteristics of CWPs from ground tissue (GT), junction of ground and vascular tissue (JT), and vascular tissue (VT) of carrot were investigated.					
31151623	3	72	theme	galacturonic	801:812	arg1	content					819:825	galacturonic acid content	801:825	galacturonic acid content	801:825	Remarkable differences were obtained in the amount of pectic fractions, galacturonic acid content, degree of methoxylation, sugar composition and linearity of CWPs that fractionated from different tissue zones of raw carrots.					
31426994	3	0	theme	-Manp-	784:789	arg1	1→					791:792	-Manp-(1→	784:792	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	572:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	6	1	theme	anti-HBV	1216:1223	arg1	activity					1225:1232	anti-HBV activity	1216:1232	anti-HBV activity of polysaccharides	1216:1251	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	1	2	theme	polysaccharide	145:158	arg1	SLP-4					171:175	A novel polysaccharide designated SLP-4	137:175	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da	137:199	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	6	3	theme	present	1123:1129	arg1	study					1131:1135	The present study	1119:1135	The present study	1119:1135	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	1	4	theme	Saussurea	232:240	arg1	laniceps					242:249	Saussurea laniceps	232:249	Saussurea laniceps	232:249	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	0	5	theme	Saussurea	74:82	arg1	Petal					65:69	Petal	65:69	Petal	65:69	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	0	6	from	Elucidation	11:21	arg1	Petal					65:69	Petal	65:69	Petal	65:69	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	4	7	theme	HBeAg	868:872	arg1	secretion					845:853	the secretion	841:853	the secretion of HBsAg and HBeAg in HepG2.2.15 cells	841:892	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	2	8	theme	galacturonic	344:355	arg1	acid					357:360	galacturonic acid	344:360	galacturonic acid	344:360	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	3	9	theme	-Galp-	703:708	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	1	10	theme	laniceps	242:249	arg1	petal					223:227	petal	223:227	petal	223:227	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	3	11	with	polysaccharide	530:543	arg1	backbone					552:559	a backbone	550:559	a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	550:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	12	from	mannose	325:331	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	4	13	theme	DNA	943:945	arg1	replication					924:934	the replication	920:934	the replication of HBV DNA	920:945	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	3	14	theme	-Galp-	577:582	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	15	theme	1→	684:685	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	4	16	contain	had	899:901	arg1	SLP-4					809:813	SLP-4	809:813	SLP-4	809:813	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	4	16	contain	had	899:901	arg2	effect					910:915	little effect	903:915	little effect	903:915	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	3	17	theme	→3,6	572:575	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	18	theme	1→	760:761	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	19	theme	typical	515:521	arg1	polysaccharide					530:543	a typical pectin polysaccharide	513:543	a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	513:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	19	theme	typical	515:521	arg1	SLP-4					503:507	SLP-4	503:507	SLP-4	503:507	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	20	theme	1→	722:723	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	6	21	theme	polysaccharides	1237:1251	arg1	activity					1225:1232	anti-HBV activity	1216:1232	anti-HBV activity of polysaccharides	1216:1251	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	2	22	from	rhamnose	334:341	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	5	23	theme	cellular	979:986	arg1	pathways					988:995	cellular pathways	979:995	cellular pathways	979:995	This inhibition didn't involve cellular pathways, and was due to the interaction between SLP-4 and HBsAg or HBeAg, which may block the ELISA detection of HBsAg and HBeAg.					
31426994	2	24	from	acid	357:360	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	2	25	theme	composition	267:277	arg1	analysis					279:286	Monosaccharide composition analysis	252:286	Monosaccharide composition analysis	252:286	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	4	26	theme	HBsAg	858:862	arg1	secretion					845:853	the secretion	841:853	the secretion of HBsAg and HBeAg in HepG2.2.15 cells	841:892	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	2	27	theme	0.825:2.030:14.998:0.841:8.260:4.039:6.009	424:465	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	4	28	theme	HBV	939:941	arg1	DNA					943:945	HBV DNA	939:945	HBV DNA	939:945	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	4	29	theme	little	903:908	arg1	effect					910:915	little effect	903:915	little effect	903:915	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	6	30	theme	SLP-4	1189:1193	arg1	study					1180:1184	further study	1172:1184	further study of SLP-4	1172:1193	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	4	31	from	secretion	845:853	arg1	cells					888:892	HepG2.2.15 cells	877:892	HepG2.2.15 cells	877:892	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	0	32	theme	Structural	0:9	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea	0:82	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	2	33	from	glucose	363:369	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	3	34	theme	1→	584:585	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	35	theme	1→	734:735	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	→4					781:782	→4	781:782	→4	781:782	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	1→					774:775	1→	774:775	1→	774:775	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	1→					791:792	-Manp-(1→	784:792	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	572:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	→6					603:604	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	→6	603:604	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	→4					617:618	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	→4	617:618	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	1→					650:651	1→	650:651	1→ with the branches of →4	650:675	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	36	dep	-Galp-	623:628	arg1	→4					588:589	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	→4	588:589	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	0	37	theme	Anti-HBV	118:125	arg1	Activity					127:134	its Anti-HBV Activity	114:134	its Anti-HBV Activity	114:134	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	3	38	with	1→	650:651	arg1	branches					662:669	the branches	658:669	the branches of →4	658:675	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	39	theme	molar	409:413	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	1	40	theme	designated	160:169	arg1	SLP-4					171:175	A novel polysaccharide designated SLP-4	137:175	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da	137:199	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	6	41	theme	further	1172:1178	arg1	study					1180:1184	further study	1172:1184	further study of SLP-4	1172:1193	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	1	42	theme	19681 Da	192:199	arg1	Mw					186:187	the Mw	182:187	the Mw of 19681 Da	182:199	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	0	43	theme	Pectin-Polysaccharide	34:54	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea	0:82	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	3	44	theme	1→	613:614	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	0	45	theme	Novel	28:32	arg1	Pectin-Polysaccharide					34:54	a Novel Pectin-Polysaccharide	26:54	a Novel Pectin-Polysaccharide from the Petal of Saussurea	26:82	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	0	46	theme	Activity	127:134	arg1	Mechanism					101:109	and the Mechanism	93:109	Mechanism	101:109	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	5	47	theme	HBeAg	1112:1116	arg1	detection					1089:1097	the ELISA detection	1079:1097	the ELISA detection of HBsAg and HBeAg	1079:1116	This inhibition didn't involve cellular pathways, and was due to the interaction between SLP-4 and HBsAg or HBeAg, which may block the ELISA detection of HBsAg and HBeAg.					
31426994	3	48	theme	-GalpA-	591:597	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	49	from	galactose	372:380	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	3	50	theme	Structural	468:477	arg1	features					479:486	Structural features	468:486	Structural features	468:486	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	0	51	from	Petal	65:69	arg1	Pectin-Polysaccharide					34:54	a Novel Pectin-Polysaccharide	26:54	a Novel Pectin-Polysaccharide from the Petal of Saussurea	26:82	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	0	51	from	Petal	65:69	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea	0:82	Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity.					
31426994	6	52	theme	activity	1225:1232	arg1	understanding					1199:1211	understanding	1199:1211	understanding of anti-HBV activity of polysaccharides	1199:1251	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	6	52	theme	activity	1225:1232	arg1	information					1156:1166	useful information	1149:1166	useful information for further study of SLP-4	1149:1193	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	5	53	theme	ELISA	1083:1087	arg1	detection					1089:1097	the ELISA detection	1079:1097	the ELISA detection of HBsAg and HBeAg	1079:1116	This inhibition didn't involve cellular pathways, and was due to the interaction between SLP-4 and HBsAg or HBeAg, which may block the ELISA detection of HBsAg and HBeAg.					
31426994	3	54	theme	-Galp-	606:611	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	55	from	xylose	383:388	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	3	56	contain	containing	561:570	arg2	-Rhap-					643:648	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Rhap-	643:648	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	56	contain	containing	561:570	arg1	backbone					552:559	a backbone	550:559	a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	550:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	56	contain	containing	561:570	arg2	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	1	57	with	SLP-4	171:175	arg1	Mw					186:187	the Mw	182:187	the Mw of 19681 Da	182:199	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	3	58	theme	pectin	523:528	arg1	polysaccharide					530:543	a typical pectin polysaccharide	513:543	a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→	513:792	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	58	theme	pectin	523:528	arg1	SLP-4					503:507	SLP-4	503:507	SLP-4	503:507	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	59	theme	Monosaccharide	252:265	arg1	analysis					279:286	Monosaccharide composition analysis	252:286	Monosaccharide composition analysis	252:286	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	5	60	theme	HBsAg	1102:1106	arg1	detection					1089:1097	the ELISA detection	1079:1097	the ELISA detection of HBsAg and HBeAg	1079:1116	This inhibition didn't involve cellular pathways, and was due to the interaction between SLP-4 and HBsAg or HBeAg, which may block the ELISA detection of HBsAg and HBeAg.					
31426994	4	61	theme	HepG2.2.15	877:886	arg1	cells					888:892	HepG2.2.15 cells	877:892	HepG2.2.15 cells	877:892	Additionally, SLP-4 could effectively inhibit the secretion of HBsAg and HBeAg in HepG2.2.15 cells, but had little effect on the replication of HBV DNA.					
31426994	3	62	theme	-Galp-	677:682	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	63	theme	-Araf-	741:746	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	2	64	from	arabinose	394:402	arg1	ratio					415:419	a molar ratio	407:419	a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009	407:465	Monosaccharide composition analysis indicated that SLP-4 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.825:2.030:14.998:0.841:8.260:4.039:6.009.					
31426994	3	65	theme	1→	696:697	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	→4					764:765	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	→4	764:765	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	→3					700:701	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	→3	700:701	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	T-Rhap-					714:720	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	T-Rhap-	714:720	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	T-Glcp-					752:758	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	T-Glcp-	752:758	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	→5					738:739	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	→5	738:739	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	T-Galp-					688:694	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	T-Galp-	688:694	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	66	dep	-Xylp-	767:772	arg1	T-Araf-					726:732	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	T-Araf-	726:732	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	6	67	theme	useful	1149:1154	arg1	information					1156:1166	useful information	1149:1166	useful information for further study of SLP-4	1149:1193	The present study may provide useful information for further study of SLP-4 and understanding of anti-HBV activity of polysaccharides.					
31426994	3	68	theme	→4	674:675	arg1	branches					662:669	the branches	658:669	the branches of →4	658:675	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	69	theme	1→	599:600	arg1	-Galp-					623:628	→3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-	572:648	-Galp-	623:628	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	3	70	theme	1→	748:749	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
31426994	1	71	theme	novel	139:143	arg1	SLP-4					171:175	A novel polysaccharide designated SLP-4	137:175	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da	137:199	A novel polysaccharide designated SLP-4 with the Mw of 19681 Da was purified from the petal of Saussurea laniceps.					
31426994	3	72	theme	1→	710:711	arg1	-Xylp-					767:772	-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-	677:772	-Xylp-	767:772	Structural features indicated that SLP-4 was a typical pectin polysaccharide with a backbone containing →3,6)-Galp-(1→, →4)-GalpA-(1→, →6)-Galp-(1→, →4, 6)-Galp-(1→ and →2, 4)-Rhap-(1→ with the branches of →4)-Galp-(1→, T-Galp-(1→, →3)-Galp-(1→, T-Rhap-(1→, T-Araf-(1→, →5)-Araf-(1→, T-Glcp-(1→, →4)-Xylp-(1→ and →4)-Manp-(1→.					
29954476	16	0	theme	different	2319:2327	arg1	curves					2341:2346	the different calibration curves	2315:2346	the different calibration curves	2315:2346	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	13	1	theme	4.4	1861:1863	arg1	factors					1850:1856	factors	1850:1856	factors of 4.4 and 4.2, and 2.4 and 1.6	1850:1888	Both overestimated the MW of 1 and 2 by factors of 4.4 and 4.2, and 2.4 and 1.6, when using dextran and pullulan calibration, respectively.					
29954476	15	2	theme	broad	2171:2175	arg1	standards					2189:2197	broad calibration standards	2171:2197	broad calibration standards	2171:2197	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	10	3	located	observed	1519:1526	arg1	polysaccharides					1541:1555	some aloe polysaccharides	1531:1555	some aloe polysaccharides in the current analysis	1531:1579	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	10	3	located	observed	1519:1526	arg2	interactions					1463:1474	interactions	1463:1474	interactions between polysaccharide molecules	1463:1507	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	10	3	located	observed	1519:1526	arg2	effects					1451:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	13	4	theme	4.2	1869:1871	arg1	factors					1850:1856	factors	1850:1856	factors of 4.4 and 4.2, and 2.4 and 1.6	1850:1888	Both overestimated the MW of 1 and 2 by factors of 4.4 and 4.2, and 2.4 and 1.6, when using dextran and pullulan calibration, respectively.					
29954476	9	5	theme	analytical	1383:1392	arg1	environment					1394:1404	the analytical environment	1379:1404	the analytical environment of a 0.1 M NaCl solution	1379:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	8	6	theme	reliable	1196:1203	arg1	results					1205:1211	reliable results	1196:1211	reliable results	1196:1211	The conditions to obtain reliable results from MALS measurement were examined.					
29954476	13	7	theme	2	1845:1845	arg1	MW					1833:1834	the MW	1829:1834	the MW of 1 and 2	1829:1845	Both overestimated the MW of 1 and 2 by factors of 4.4 and 4.2, and 2.4 and 1.6, when using dextran and pullulan calibration, respectively.					
29954476	0	8	theme	Molecular	0:8	arg1	Determination					17:29	Molecular Weight Determination	0:29	Molecular Weight Determination of Aloe Polysaccharides	0:53	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	1	9	theme	chromatography	198:211	arg1	RI					237:238	RI	237:238	RI	237:238	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	9	theme	chromatography	198:211	arg1	index					230:234	Size exclusion chromatography (SEC)/refractive index	183:234	Size exclusion chromatography (SEC)/refractive index (RI)	183:239	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	16	10	theme	determined	2357:2366	arg1	MWs					2368:2370	the determined MWs	2353:2370	the determined MWs	2353:2370	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	4	11	theme	recent	665:670	arg1	technologies					672:683	more recent technologies	660:683	more recent technologies	660:683	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	14	12	theme	similar	2030:2036	arg1	result					2038:2043	a similar result	2028:2043	a similar result	2028:2043	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	9	13	theme	0.1	1411:1413	arg1	M					1415:1415	M	1415:1415	M	1415:1415	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	15	14	theme	calibration	2253:2263	arg1	standards					2265:2273	narrow polydispersity pullulan calibration standards	2222:2273	narrow polydispersity pullulan calibration standards	2222:2273	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	9	15	theme	NaCl	1417:1420	arg1	solution					1422:1429	a 0.1 M NaCl solution	1409:1429	a 0.1 M NaCl solution	1409:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	4	16	theme	traditional	636:646	arg1	methods					648:654	the traditional methods	632:654	the traditional methods	632:654	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	14	17	theme	3.1	2019:2021	arg1	factor					2009:2014	a factor	2007:2014	a factor of 3.1	2007:2021	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	0	18	theme	Index	154:158	arg1	Detectors					160:168	Multi-Angle Laser Light Scattering and Refractive Index Detectors	104:168	Multi-Angle Laser Light Scattering and Refractive Index Detectors	104:168	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	15	19	theme	polydispersity	2229:2242	arg1	standards					2265:2273	narrow polydispersity pullulan calibration standards	2222:2273	narrow polydispersity pullulan calibration standards	2222:2273	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	12	20	from	differences	1776:1786	arg1	MWD					1795:1797	the MWD	1791:1797	the MWD	1791:1797	Comparing the results with SEC/RI calibrated by pullulan and dextran standards, marked differences in the MWD are found.					
29954476	3	21	used	used	584:587	arg2	methods					571:577	different methods	561:577	different methods	561:577	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	17	22	theme	polysaccharide	2492:2505	arg1	standards					2507:2515	true polysaccharide standards	2487:2515	true polysaccharide standards	2487:2515	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	3	23	dep	MW	520:521	arg1	the					516:518	the	516:518	the	516:518	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	6	24	theme	1-3	953:955	arg1	glucomannans					993:1004	1, 4-β-linked glucomannans	979:1004	glucomannans	993:1004	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	6	24	theme	1-3	953:955	arg1	structures					939:948	The chemical structures	926:948	The chemical structures of 1-3	926:955	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	17	25	theme	become	2517:2522	arg1	MWD					2541:2543	MWD's	2541:2545	MWD's	2541:2545	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	17	25	theme	become	2517:2522	arg1	standards					2578:2586	the standards	2574:2586	the standards employed	2574:2595	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	17	25	theme	become	2517:2522	arg1	MW					2534:2535	become available MW	2517:2535	become available MW	2517:2535	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	0	26	theme	Exclusion	66:74	arg1	Chromatography					76:89	Size Exclusion Chromatography	61:89	Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors	61:168	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	15	27	theme	isolated	2121:2128	arg1	polysaccharides					2135:2149	The two isolated aloe polysaccharides	2113:2149	The two isolated aloe polysaccharides	2113:2149	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	1	28	theme	Size	183:186	arg1	SEC					214:216	SEC	214:216	SEC	214:216	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	28	theme	Size	183:186	arg1	chromatography					198:211	Size exclusion chromatography	183:211	Size exclusion chromatography (SEC)/refractive index (RI)	183:239	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	14	29	theme	3	2002:2002	arg1	MW					1996:1997	the MW	1992:1997	the MW of 3	1992:2002	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	0	30	theme	Multi-Angle	104:114	arg1	Scattering					128:137	Multi-Angle Laser Light Scattering	104:137	Multi-Angle Laser Light Scattering	104:137	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	0	31	theme	Light	122:126	arg1	Scattering					128:137	Multi-Angle Laser Light Scattering	104:137	Multi-Angle Laser Light Scattering	104:137	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	5	32	theme	vera	915:918	arg1	leaf					920:923	A. vera leaf	912:923	A. vera leaf	912:923	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	4	33	theme	Aloe	721:724	arg1	polysaccharides					731:745	Aloe vera polysaccharides	721:745	Aloe vera polysaccharides	721:745	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	5	34	theme	light	790:794	arg1	MALS					808:811	MALS	808:811	MALS	808:811	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	5	34	theme	light	790:794	arg1	scattering					796:805	multi-angle laser light scattering	772:805	multi-angle laser light scattering (MALS) detection	772:822	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	6	35	theme	4-β-linked	982:991	arg1	glucomannans					993:1004	1, 4-β-linked glucomannans	979:1004	glucomannans	993:1004	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	6	35	theme	4-β-linked	982:991	arg1	structures					939:948	The chemical structures	926:948	The chemical structures of 1-3	926:955	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	14	36	theme	pullulan	1956:1963	arg1	calibration					1965:1975	pullulan calibration	1956:1975	pullulan calibration	1956:1975	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	4	37	theme	polysaccharides	731:745	arg1	MW					707:708	MW	707:708	MW	707:708	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	4	37	theme	polysaccharides	731:745	arg1	MWD					714:716	MWD	714:716	MWD	714:716	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	9	38	theme	4-β-linked	1298:1307	arg1	glucomannan					1309:1319	the 1, 4-β-linked glucomannan	1291:1319	glucomannan	1309:1319	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	2	39	theme	calibration	467:477	arg1	standards					479:487	calibration standards	467:487	calibration standards	467:487	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	2	39	theme	calibration	467:477	arg1	dextran					429:435	dextran	429:435	dextran	429:435	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	2	39	theme	calibration	467:477	arg1	pullulan					416:423	commercially available pullulan	393:423	commercially available pullulan	393:423	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	10	40	theme	exclusion	1441:1449	arg1	effects					1451:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	0	41	theme	Aloe	34:37	arg1	Polysaccharides					39:53	Aloe Polysaccharides	34:53	Aloe Polysaccharides	34:53	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	1	42	theme	molecular	290:298	arg1	MWD					322:324	MWD	322:324	MWD	322:324	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	42	theme	molecular	290:298	arg1	distributions					307:319	molecular weight distributions	290:319	molecular weight distributions (MWD) of polysaccharides	290:344	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	3	43	theme	Significant	490:500	arg1	difference					502:511	Significant difference	490:511	Significant difference in the MW and MWD	490:529	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	5	44	theme	multi-angle	772:782	arg1	MALS					808:811	MALS	808:811	MALS	808:811	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	5	44	theme	multi-angle	772:782	arg1	scattering					796:805	multi-angle laser light scattering	772:805	multi-angle laser light scattering (MALS) detection	772:822	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	1	45	used	used	246:249	arg2	RI					237:238	RI	237:238	RI	237:238	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	45	used	used	246:249	arg2	index					230:234	Size exclusion chromatography (SEC)/refractive index	183:234	Size exclusion chromatography (SEC)/refractive index (RI)	183:239	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	16	46	theme	aloe	2284:2287	arg1	samples					2289:2295	Several aloe samples	2276:2295	Several aloe samples	2276:2295	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	9	47	theme	random	1347:1352	arg1	coils					1354:1358	random coils	1347:1358	random coils	1347:1358	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	16	48	theme	MALS	2415:2418	arg1	measurement					2420:2430	MALS measurement	2415:2430	MALS measurement	2415:2430	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	9	49	link	4-β-linked	1298:1307	arg1	glucomannan					1309:1319	the 1, 4-β-linked glucomannan	1291:1319	glucomannan	1309:1319	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	14	50	theme	MALS	2095:2098	arg1	measurement					2100:2110	MALS measurement	2095:2110	MALS measurement	2095:2110	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	2	51	theme	product	355:361	arg1	research					363:370	aloe product research	350:370	aloe product research	350:370	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	15	52	theme	calibration	2177:2187	arg1	standards					2189:2197	broad calibration standards	2171:2197	broad calibration standards	2171:2197	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	16	53	theme	calibration	2329:2339	arg1	curves					2341:2346	the different calibration curves	2315:2346	the different calibration curves	2315:2346	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	6	54	theme	glycosidic	1040:1049	arg1	analysis					1059:1066	glycosidic linkage analysis	1040:1066	glycosidic linkage analysis	1040:1066	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	6	55	link	4-β-linked	982:991	arg1	glucomannans					993:1004	1, 4-β-linked glucomannans	979:1004	glucomannans	993:1004	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	6	55	link	4-β-linked	982:991	arg1	structures					939:948	The chemical structures	926:948	The chemical structures of 1-3	926:955	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	7	56	theme	Absolute	1069:1076	arg1	MW					1078:1079	Absolute MW	1069:1079	Absolute MW	1069:1079	Absolute MW and root-mean-square radius were determined by MALS on the isolated aloe polysaccharides.					
29954476	11	57	theme	weight-average	1586:1599	arg1	MW					1601:1602	The weight-average MW	1582:1602	The weight-average MW obtained by MALS measurement for 1, 2, and 3	1582:1647	The weight-average MW obtained by MALS measurement for 1, 2, and 3 are 55, 129, and 962 kDa, respectively.					
29954476	11	57	theme	weight-average	1586:1599	arg1	kDa					1670:1672	55, 129, and 962 kDa	1653:1672	55, 129, and 962 kDa	1653:1672	The weight-average MW obtained by MALS measurement for 1, 2, and 3 are 55, 129, and 962 kDa, respectively.					
29954476	9	58	theme	spheres	1368:1374	arg1	conformation					1331:1342	the conformation	1327:1342	the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution	1327:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	14	59	theme	dextran	2063:2069	arg1	calibration					2071:2081	dextran calibration	2063:2081	dextran calibration	2063:2081	Using pullulan calibration underestimated the MW of 3 by a factor of 3.1, but a similar result was obtained from dextran calibration compared to MALS measurement.					
29954476	11	60	theme	MALS	1616:1619	arg1	measurement					1621:1631	MALS measurement	1616:1631	MALS measurement for 1, 2, and 3	1616:1647	The weight-average MW obtained by MALS measurement for 1, 2, and 3 are 55, 129, and 962 kDa, respectively.					
29954476	1	61	theme	molecular	264:272	arg1	MW					282:283	MW	282:283	MW	282:283	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	61	theme	molecular	264:272	arg1	weight					274:279	molecular weight	264:279	molecular weight (MW)	264:284	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	10	62	theme	aloe	1536:1539	arg1	polysaccharides					1541:1555	some aloe polysaccharides	1531:1555	some aloe polysaccharides in the current analysis	1531:1579	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	1	63	theme	exclusion	188:196	arg1	SEC					214:216	SEC	214:216	SEC	214:216	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	63	theme	exclusion	188:196	arg1	chromatography					198:211	Size exclusion chromatography	183:211	Size exclusion chromatography (SEC)/refractive index (RI)	183:239	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	7	64	theme	root-mean-square	1085:1100	arg1	radius					1102:1107	root-mean-square radius	1085:1107	root-mean-square radius	1085:1107	Absolute MW and root-mean-square radius were determined by MALS on the isolated aloe polysaccharides.					
29954476	3	65	theme	different	561:569	arg1	methods					571:577	different methods	561:577	different methods	561:577	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	10	66	theme	current	1564:1570	arg1	analysis					1572:1579	the current analysis	1560:1579	the current analysis	1560:1579	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	0	67	theme	Refractive	143:152	arg1	Index					154:158	Refractive Index	143:158	Refractive Index	143:158	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	3	68	from	difference	502:511	arg1	MWD					527:529	MWD	527:529	MWD	527:529	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	3	68	from	difference	502:511	arg1	MW					520:521	MW	520:521	MW	520:521	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	3	69	located	found	536:540	arg2	difference					502:511	Significant difference	490:511	Significant difference in the MW and MWD	490:529	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	3	69	located	found	536:540	arg1	literature					545:554	literature	545:554	literature	545:554	Significant difference in the MW and MWD were found in literature when different methods were used.					
29954476	9	70	theme	M	1415:1415	arg1	solution					1422:1429	a 0.1 M NaCl solution	1409:1429	a 0.1 M NaCl solution	1409:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	8	71	theme	MALS	1218:1221	arg1	measurement					1223:1233	MALS measurement	1218:1233	MALS measurement	1218:1233	The conditions to obtain reliable results from MALS measurement were examined.					
29954476	9	72	theme	solution	1422:1429	arg1	environment					1394:1404	the analytical environment	1379:1404	the analytical environment of a 0.1 M NaCl solution	1379:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	7	73	theme	aloe	1149:1152	arg1	polysaccharides					1154:1168	the isolated aloe polysaccharides	1136:1168	the isolated aloe polysaccharides	1136:1168	Absolute MW and root-mean-square radius were determined by MALS on the isolated aloe polysaccharides.					
29954476	15	74	theme	pullulan	2244:2251	arg1	standards					2265:2273	narrow polydispersity pullulan calibration standards	2222:2273	narrow polydispersity pullulan calibration standards	2222:2273	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	9	75	theme	MALS	1259:1262	arg1	analysis					1264:1271	MALS analysis	1259:1271	MALS analysis	1259:1271	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	17	76	theme	true	2487:2490	arg1	standards					2507:2515	true polysaccharide standards	2487:2515	true polysaccharide standards	2487:2515	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	15	77	theme	narrow	2222:2227	arg1	standards					2265:2273	narrow polydispersity pullulan calibration standards	2222:2273	narrow polydispersity pullulan calibration standards	2222:2273	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	13	78	theme	1	1839:1839	arg1	MW					1833:1834	the MW	1829:1834	the MW of 1 and 2	1829:1845	Both overestimated the MW of 1 and 2 by factors of 4.4 and 4.2, and 2.4 and 1.6, when using dextran and pullulan calibration, respectively.					
29954476	17	79	theme	available	2524:2532	arg1	MWD					2541:2543	MWD's	2541:2545	MWD's	2541:2545	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	17	79	theme	available	2524:2532	arg1	standards					2578:2586	the standards	2574:2586	the standards employed	2574:2595	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	17	79	theme	available	2524:2532	arg1	MW					2534:2535	become available MW	2517:2535	become available MW	2517:2535	Conclusions: The results clearly indicated that until true polysaccharide standards become available MW and MWD's will be simply relative to the standards employed and the technologies used.					
29954476	9	80	from	conformation	1331:1342	arg1	environment					1394:1404	the analytical environment	1379:1404	the analytical environment of a 0.1 M NaCl solution	1379:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	6	81	theme	chemical	930:937	arg1	glucomannans					993:1004	1, 4-β-linked glucomannans	979:1004	glucomannans	993:1004	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	6	81	theme	chemical	930:937	arg1	structures					939:948	The chemical structures	926:948	The chemical structures of 1-3	926:955	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	0	82	theme	Laser	116:120	arg1	Scattering					128:137	Multi-Angle Laser Light Scattering	104:137	Multi-Angle Laser Light Scattering	104:137	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	1	83	theme	polysaccharides	330:344	arg1	MWD					322:324	MWD	322:324	MWD	322:324	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	83	theme	polysaccharides	330:344	arg1	distributions					307:319	molecular weight distributions	290:319	molecular weight distributions (MWD) of polysaccharides	290:344	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	83	theme	polysaccharides	330:344	arg1	MW					282:283	MW	282:283	MW	282:283	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	83	theme	polysaccharides	330:344	arg1	weight					274:279	molecular weight	264:279	molecular weight (MW)	264:284	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	0	84	theme	Scattering	128:137	arg1	Detectors					160:168	Multi-Angle Laser Light Scattering and Refractive Index Detectors	104:168	Multi-Angle Laser Light Scattering and Refractive Index Detectors	104:168	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	13	85	theme	pullulan	1914:1921	arg1	calibration					1923:1933	pullulan calibration	1914:1933	pullulan calibration	1914:1933	Both overestimated the MW of 1 and 2 by factors of 4.4 and 4.2, and 2.4 and 1.6, when using dextran and pullulan calibration, respectively.					
29954476	1	86	theme	/refractive	218:228	arg1	RI					237:238	RI	237:238	RI	237:238	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	86	theme	/refractive	218:228	arg1	index					230:234	Size exclusion chromatography (SEC)/refractive index	183:234	Size exclusion chromatography (SEC)/refractive index (RI)	183:239	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	5	87	theme	A.	912:913	arg1	leaf					920:923	A. vera leaf	912:923	A. vera leaf	912:923	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	6	88	theme	monosaccharide	1009:1022	arg1	composition					1024:1034	monosaccharide composition	1009:1034	monosaccharide composition	1009:1034	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	7	89	from	MALS	1128:1131	arg1	polysaccharides					1154:1168	the isolated aloe polysaccharides	1136:1168	the isolated aloe polysaccharides	1136:1168	Absolute MW and root-mean-square radius were determined by MALS on the isolated aloe polysaccharides.					
29954476	2	90	theme	quality	376:382	arg1	control					384:390	quality control	376:390	quality control	376:390	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	0	91	theme	Weight	10:15	arg1	Determination					17:29	Molecular Weight Determination	0:29	Molecular Weight Determination of Aloe Polysaccharides	0:53	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	5	92	theme	scattering	796:805	arg1	detection					814:822	multi-angle laser light scattering (MALS) detection	772:822	multi-angle laser light scattering (MALS) detection	772:822	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	4	93	dep	MW	707:708	arg1	the					703:705	the	703:705	the	703:705	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	8	94	dep	conditions	1175:1184	arg1	obtain					1189:1194	obtain	1189:1194	to obtain reliable results from MALS measurement	1186:1233	The conditions to obtain reliable results from MALS measurement were examined.					
29954476	4	95	theme	vera	726:729	arg1	polysaccharides					731:745	Aloe vera polysaccharides	721:745	Aloe vera polysaccharides	721:745	Objectives: This study was to investigate the traditional methods and more recent technologies used to determine the MW and MWD of Aloe vera polysaccharides.					
29954476	2	96	theme	available	406:414	arg1	standards					479:487	calibration standards	467:487	calibration standards	467:487	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	2	96	theme	available	406:414	arg1	dextran					429:435	dextran	429:435	dextran	429:435	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	2	96	theme	available	406:414	arg1	pullulan					416:423	commercially available pullulan	393:423	commercially available pullulan	393:423	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	0	97	theme	Polysaccharides	39:53	arg1	Determination					17:29	Molecular Weight Determination	0:29	Molecular Weight Determination of Aloe Polysaccharides	0:53	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	15	98	theme	aloe	2130:2133	arg1	polysaccharides					2135:2149	The two isolated aloe polysaccharides	2113:2149	The two isolated aloe polysaccharides	2113:2149	The two isolated aloe polysaccharides were employed to be broad calibration standards or to be combined with narrow polydispersity pullulan calibration standards.					
29954476	10	99	theme	Non-size	1432:1439	arg1	effects					1451:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects	1432:1457	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	16	100	theme	Several	2276:2282	arg1	samples					2289:2295	Several aloe samples	2276:2295	Several aloe samples	2276:2295	Several aloe samples were tested using the different calibration curves, and the determined MWs were compared with the results obtained by MALS measurement.					
29954476	7	101	theme	isolated	1140:1147	arg1	polysaccharides					1154:1168	the isolated aloe polysaccharides	1136:1168	the isolated aloe polysaccharides	1136:1168	Absolute MW and root-mean-square radius were determined by MALS on the isolated aloe polysaccharides.					
29954476	0	102	theme	Size	61:64	arg1	Chromatography					76:89	Size Exclusion Chromatography	61:89	Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors	61:168	Molecular Weight Determination of Aloe Polysaccharides Using Size Exclusion Chromatography Coupled with Multi-Angle Laser Light Scattering and Refractive Index Detectors.					
29954476	1	103	theme	weight	300:305	arg1	MWD					322:324	MWD	322:324	MWD	322:324	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	1	103	theme	weight	300:305	arg1	distributions					307:319	molecular weight distributions	290:319	molecular weight distributions (MWD) of polysaccharides	290:344	Background: Size exclusion chromatography (SEC)/refractive index (RI) were used to determine molecular weight (MW) and molecular weight distributions (MWD) of polysaccharides.					
29954476	12	104	theme	marked	1769:1774	arg1	differences					1776:1786	marked differences	1769:1786	marked differences in the MWD	1769:1797	Comparing the results with SEC/RI calibrated by pullulan and dextran standards, marked differences in the MWD are found.					
29954476	12	105	theme	pullulan	1737:1744	arg1	standards					1758:1766	pullulan and dextran standards	1737:1766	pullulan and dextran standards	1737:1766	Comparing the results with SEC/RI calibrated by pullulan and dextran standards, marked differences in the MWD are found.					
29954476	10	106	theme	polysaccharide	1484:1497	arg1	molecules					1499:1507	polysaccharide molecules	1484:1507	polysaccharide molecules	1484:1507	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	12	107	theme	dextran	1750:1756	arg1	standards					1758:1766	pullulan and dextran standards	1737:1766	pullulan and dextran standards	1737:1766	Comparing the results with SEC/RI calibrated by pullulan and dextran standards, marked differences in the MWD are found.					
29954476	2	108	theme	aloe	350:353	arg1	research					363:370	aloe product research	350:370	aloe product research	350:370	In aloe product research and quality control, commercially available pullulan and dextran are most commonly employed as calibration standards.					
29954476	9	109	theme	coils	1354:1358	arg1	conformation					1331:1342	the conformation	1327:1342	the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution	1327:1429	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
29954476	10	110	from	polysaccharides	1541:1555	arg1	analysis					1572:1579	the current analysis	1560:1579	the current analysis	1560:1579	Non-size exclusion effects and interactions between polysaccharide molecules were also observed in some aloe polysaccharides in the current analysis.					
29954476	6	111	theme	linkage	1051:1057	arg1	analysis					1059:1066	glycosidic linkage analysis	1040:1066	glycosidic linkage analysis	1040:1066	The chemical structures of 1-3 were characterized as 1, 4-β-linked glucomannans by monosaccharide composition and glycosidic linkage analysis.					
29954476	5	112	theme	laser	784:788	arg1	MALS					808:811	MALS	808:811	MALS	808:811	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	5	112	theme	laser	784:788	arg1	scattering					796:805	multi-angle laser light scattering	772:805	multi-angle laser light scattering (MALS) detection	772:822	Methods: In this study, multi-angle laser light scattering (MALS) detection was studied on three polysaccharides, 1, 2, and 3, that were isolated and purified from A. vera leaf.					
29954476	9	113	theme	hard	1363:1366	arg1	spheres					1368:1374	hard spheres	1363:1374	hard spheres	1363:1374	Results: MALS analysis demonstrates that the 1, 4-β-linked glucomannan adopt the conformation of random coils or hard spheres in the analytical environment of a 0.1 M NaCl solution.					
30014879	7	0	theme	repeating	1312:1320	arg1	unit					1322:1325	the repeating unit	1308:1325	the repeating unit	1308:1325	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	0	1	contain	contain	71:77	arg1	polysaccharides					55:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	0	1	contain	contain	71:77	arg2	di-N-acetyl-bacillosamine					79:103	di-N-acetyl-bacillosamine	79:103	di-N-acetyl-bacillosamine	79:103	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	7	2	theme	cps	1209:1211	arg1	loci					1213:1216	these serotypes' cps loci	1192:1216	these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit	1192:1325	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	7	3	theme	unit	1322:1325	arg1	biosynthesis					1292:1303	the biosynthesis	1288:1303	the biosynthesis of the repeating unit	1288:1325	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	3	4	theme	capsular	356:363	arg1	factor					415:420	a major bacterial virulence factor	387:420	a major bacterial virulence factor	387:420	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	4	theme	capsular	356:363	arg1	CPS					381:383	CPS	381:383	CPS	381:383	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	4	theme	capsular	356:363	arg1	polysaccharide					365:378	serotype 3 capsular polysaccharide	345:378	serotype 3 capsular polysaccharide (CPS)	345:384	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	4	5	theme	Chemical	494:501	arg1	data					521:524	Chemical and spectroscopic data	494:524	Chemical and spectroscopic data	494:524	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	6	dep	S.	455:456	arg1	suis					458:461	suis	458:461	suis	458:461	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	7	7	theme	putative	1227:1234	arg1	glycosyltransferases					1236:1255	putative glycosyltransferases	1227:1255	putative glycosyltransferases	1227:1255	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	6	8	theme	d-QuiNAc4NAc	1096:1107	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	8	theme	d-QuiNAc4NAc	1096:1107	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	3	9	theme	polysaccharide	365:378	arg1	structure					332:340	structure	332:340	structure	332:340	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	9	theme	polysaccharide	365:378	arg1	composition					316:326	chemical composition	307:326	chemical composition	307:326	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	9	theme	polysaccharide	365:378	arg1	antigen					430:436	the antigen	426:436	the antigen at the origin of S. suis classification into serotypes	426:491	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	6	10	theme	[d-Glc	1052:1057	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	10	theme	[d-Glc	1052:1057	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	5	11	from	genes	951:955	arg1	serotype					968:975	S. suis serotype 18	960:978	S. suis serotype 18	960:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	8	12	theme	S.	1493:1494	arg1	serotypes					1501:1509	S. suis serotypes 3 and 18	1493:1518	serotypes	1501:1509	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	3	13	theme	serotype	345:352	arg1	factor					415:420	a major bacterial virulence factor	387:420	a major bacterial virulence factor	387:420	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	13	theme	serotype	345:352	arg1	CPS					381:383	CPS	381:383	CPS	381:383	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	13	theme	serotype	345:352	arg1	polysaccharide					365:378	serotype 3 capsular polysaccharide	345:378	serotype 3 capsular polysaccharide (CPS)	345:384	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	4	14	theme	first	653:657	arg1	this					641:644	this	641:644	this	641:644	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	14	theme	first	653:657	arg1	report					659:664	the first report	649:664	the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	649:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	15	theme	diamino	783:789	arg1	sugar					791:795	this rare diamino sugar	773:795	this rare diamino sugar	773:795	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	5	16	theme	homologues	912:921	arg1	identification					894:907	the identification	890:907	the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18	890:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	1	17	theme	clinical	183:190	arg1	disease					192:198	clinical disease	183:198	clinical disease	183:198	Streptococcus suis serotype 3 is counted among the S. suis serotypes causing clinical disease in pigs.					
30014879	0	18	theme	serotype	34:41	arg1	polysaccharides					55:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	7	19	theme	responsible	1272:1282	arg1	glycosyltransferases					1236:1255	putative glycosyltransferases	1227:1255	putative glycosyltransferases	1227:1255	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	0	20	theme	suis	14:17	arg1	serotype					19:26	Streptococcus suis serotype 3	0:28	Streptococcus suis serotype 3	0:28	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	8	21	theme	composition	1387:1397	arg1	Knowledge					1356:1364	Knowledge	1356:1364	Knowledge of CPS structure and composition	1356:1397	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	8	22	from	role	1437:1440	arg1	pathogenesis					1477:1488	the pathogenesis	1473:1488	the pathogenesis of S. suis serotypes 3 and 18	1473:1518	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	5	23	theme	genes	951:955	arg1	homologues					912:921	homologues	912:921	homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18	912:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	1	24	theme	Streptococcus	106:118	arg1	serotype					125:132	Streptococcus suis serotype 3	106:134	Streptococcus suis serotype 3	106:134	Streptococcus suis serotype 3 is counted among the S. suis serotypes causing clinical disease in pigs.					
30014879	4	25	theme	Gram-positive	820:832	arg1	species					844:850	a Gram-positive bacterial species	818:850	a Gram-positive bacterial species since its initial report	818:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	6	26	theme	unit	1001:1004	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	26	theme	unit	1001:1004	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	0	27	theme	Streptococcus	0:12	arg1	serotype					19:26	Streptococcus suis serotype 3	0:28	Streptococcus suis serotype 3	0:28	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	4	28	theme	unit	545:548	arg1	sequence					550:557	the repeating unit sequence	531:557	the repeating unit sequence for serotype 3	531:572	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	1	29	theme	suis	120:123	arg1	serotype					125:132	Streptococcus suis serotype 3	106:134	Streptococcus suis serotype 3	106:134	Streptococcus suis serotype 3 is counted among the S. suis serotypes causing clinical disease in pigs.					
30014879	5	30	from	serotype	968:975	arg1	homologues					912:921	homologues	912:921	homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18	912:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	6	31	theme	repeating	991:999	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	31	theme	repeating	991:999	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	4	32	theme	repeating	535:543	arg1	sequence					550:557	the repeating unit sequence	531:557	the repeating unit sequence for serotype 3	531:572	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	33	dep	composition	316:326	arg1	the					303:305	the	303:305	the	303:305	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	1	34	dep	S.	157:158	arg1	suis					160:163	suis	160:163	suis	160:163	Streptococcus suis serotype 3 is counted among the S. suis serotypes causing clinical disease in pigs.					
30014879	5	35	theme	S.	960:961	arg1	serotype					968:975	S. suis serotype 18	960:978	S. suis serotype 18	960:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	4	36	theme	d-QuiNAc4NAc	591:602	arg1	n.					608:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n.	575:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	575:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	0	37	theme	serotype	19:26	arg1	polysaccharides					55:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	8	38	theme	structure	1373:1381	arg1	Knowledge					1356:1364	Knowledge	1356:1364	Knowledge of CPS structure and composition	1356:1397	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	6	39	theme	d-GalA4OAc	1066:1075	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	39	theme	d-GalA4OAc	1066:1075	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	40	theme	α1-3	1047:1050	arg1	[d-Glc					1052:1057	[3)d-GalNAc(α1-3)[d-Glc	1035:1057	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	40	theme	α1-3	1047:1050	arg1	β1-2					1060:1063	β1-2	1060:1063	β1-2	1060:1063	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	4	41	dep	time	768:771	arg1	observed					806:813	observed	806:813	has been observed in a Gram-positive bacterial species since its initial report	797:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	42	theme	bacterial	395:403	arg1	factor					415:420	a major bacterial virulence factor	387:420	a major bacterial virulence factor	387:420	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	42	theme	bacterial	395:403	arg1	polysaccharide					365:378	serotype 3 capsular polysaccharide	345:378	serotype 3 capsular polysaccharide (CPS)	345:384	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	7	43	theme	suis	1141:1144	arg1	serotypes					1146:1154	S. suis serotypes 3 and 18 CPS sequences and genes	1138:1187	serotypes	1146:1154	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	7	44	theme	S.	1138:1139	arg1	serotypes					1146:1154	S. suis serotypes 3 and 18 CPS sequences and genes	1138:1187	serotypes	1146:1154	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	4	45	theme	second	761:766	arg1	time					768:771	the second time	757:771	the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	757:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	46	theme	β1-	604:606	arg1	n.					608:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n.	575:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	575:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	47	theme	virulence	405:413	arg1	factor					415:420	a major bacterial virulence factor	387:420	a major bacterial virulence factor	387:420	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	47	theme	virulence	405:413	arg1	polysaccharide					365:378	serotype 3 capsular polysaccharide	345:378	serotype 3 capsular polysaccharide (CPS)	345:384	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	7	48	theme	CPS	1165:1167	arg1	sequences					1169:1177	18 CPS sequences	1162:1177	18 CPS sequences	1162:1177	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	5	49	theme	suis	963:966	arg1	serotype					968:975	S. suis serotype 18	960:978	S. suis serotype 18	960:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	4	50	theme	di-N-acetyl-d-bacillosamine	669:695	arg1	this					641:644	this	641:644	this	641:644	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	50	theme	di-N-acetyl-d-bacillosamine	669:695	arg1	report					659:664	the first report	649:664	the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	649:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	0	51	theme	capsular	46:53	arg1	polysaccharides					55:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides	0:69	Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di-N-acetyl-bacillosamine.					
30014879	4	52	contain	containing	710:719	arg2	polysaccharides					721:735	polysaccharides	721:735	polysaccharides	721:735	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	52	contain	containing	710:719	arg1	di-N-acetyl-d-bacillosamine					669:695	di-N-acetyl-d-bacillosamine	669:695	di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	669:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	52	contain	containing	710:719	arg1	QuiNAc4NAc					698:707	QuiNAc4NAc	698:707	QuiNAc4NAc	698:707	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	53	theme	bacterial	834:842	arg1	species					844:850	a Gram-positive bacterial species	818:850	a Gram-positive bacterial species since its initial report	818:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	54	theme	classification	463:476	arg1	origin					445:450	the origin	441:450	the origin of S. suis classification into serotypes	441:491	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	55	theme	major	389:393	arg1	factor					415:420	a major bacterial virulence factor	387:420	a major bacterial virulence factor	387:420	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	55	theme	major	389:393	arg1	polysaccharide					365:378	serotype 3 capsular polysaccharide	345:378	serotype 3 capsular polysaccharide (CPS)	345:384	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	56	theme	chemical	307:314	arg1	composition					316:326	chemical composition	307:326	chemical composition	307:326	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	2	57	theme	limited	214:220	arg1	information					222:232	limited information	214:232	limited information	214:232	Yet, limited information is available on this serotype.					
30014879	4	58	theme	rare	778:781	arg1	sugar					791:795	this rare diamino sugar	773:795	this rare diamino sugar	773:795	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	3	59	from	origin	445:450	arg1	structure					332:340	structure	332:340	structure	332:340	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	59	from	origin	445:450	arg1	composition					316:326	chemical composition	307:326	chemical composition	307:326	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	3	59	from	origin	445:450	arg1	antigen					430:436	the antigen	426:436	the antigen at the origin of S. suis classification into serotypes	426:491	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	6	60	theme	d-GalNAc	1038:1045	arg1	[d-Glc					1052:1057	[3)d-GalNAc(α1-3)[d-Glc	1035:1057	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	60	theme	d-GalNAc	1038:1045	arg1	β1-2					1060:1063	β1-2	1060:1063	β1-2	1060:1063	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	3	61	theme	first	292:296	arg1	time					298:301	the first time	288:301	the first time	288:301	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	6	62	theme	d-GalNAc	1082:1089	arg1	n					1113:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n	1033:1113	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	6	62	theme	d-GalNAc	1082:1089	arg1	sequence					1006:1013	the repeating unit sequence	987:1013	the repeating unit sequence for serotype 18	987:1029	Thus, the repeating unit sequence for serotype 18 is: [3)d-GalNAc(α1-3)[d-Glc (β1-2)]d-GalA4OAc(β1-3)d-GalNAc(α1-3)d-QuiNAc4NAc(α1-]n.					
30014879	5	63	theme	UDP-QuiNAc4NAc	926:939	arg1	genes					951:955	UDP-QuiNAc4NAc synthesis genes	926:955	UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18	926:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	7	64	theme	loci	1213:1216	arg1	serotypes					1146:1154	S. suis serotypes 3 and 18 CPS sequences and genes	1138:1187	serotypes	1146:1154	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	7	64	theme	loci	1213:1216	arg1	genes					1183:1187	genes	1183:1187	genes	1183:1187	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	7	64	theme	loci	1213:1216	arg1	sequences					1169:1177	18 CPS sequences	1162:1177	18 CPS sequences	1162:1177	A correlation between S. suis serotypes 3 and 18 CPS sequences and genes of these serotypes' cps loci encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.					
30014879	3	65	theme	S.	455:456	arg1	classification					463:476	S. suis classification	455:476	S. suis classification into serotypes	455:491	Here we determined for the first time the chemical composition and structure of serotype 3 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.					
30014879	4	66	located	observed	806:813	arg1	species					844:850	a Gram-positive bacterial species	818:850	a Gram-positive bacterial species since its initial report	818:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	66	located	observed	806:813	arg2	sugar					791:795	this rare diamino sugar	773:795	this rare diamino sugar	773:795	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	67	theme	D-GlcA	578:583	arg1	n.					608:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n.	575:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	575:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	5	68	theme	synthesis	941:949	arg1	genes					951:955	UDP-QuiNAc4NAc synthesis genes	926:955	UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18	926:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	8	69	theme	18	1517:1518	arg1	pathogenesis					1477:1488	the pathogenesis	1473:1488	the pathogenesis of S. suis serotypes 3 and 18	1473:1518	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	8	70	theme	bacterial	1450:1458	arg1	component					1460:1468	this bacterial component	1445:1468	this bacterial component	1445:1468	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	8	71	theme	CPS	1369:1371	arg1	structure					1373:1381	CPS structure	1369:1381	CPS structure	1369:1381	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	4	72	theme	initial	862:868	arg1	report					870:875	its initial report	858:875	its initial report	858:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	73	dep	n.	608:609	arg1	this					641:644	this	641:644	this	641:644	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	4	73	dep	n.	608:609	arg1	report					659:664	the first report	649:664	the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	649:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	1	74	theme	S.	157:158	arg1	serotypes					165:173	the S. suis serotypes	153:173	the S. suis serotypes causing clinical disease in pigs	153:206	Streptococcus suis serotype 3 is counted among the S. suis serotypes causing clinical disease in pigs.					
30014879	8	75	theme	serotypes	1501:1509	arg1	pathogenesis					1477:1488	the pathogenesis	1473:1488	the pathogenesis of S. suis serotypes 3 and 18	1473:1518	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	4	76	theme	β1-3	586:589	arg1	n.					608:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n.	575:609	[4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report	575:875	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30014879	8	77	theme	suis	1496:1499	arg1	serotypes					1501:1509	S. suis serotypes 3 and 18	1493:1518	serotypes	1501:1509	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	8	78	theme	component	1460:1468	arg1	role					1437:1440	the role	1433:1440	the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18	1433:1518	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotypes 3 and 18.					
30014879	5	79	from	homologues	912:921	arg1	serotype					968:975	S. suis serotype 18	960:978	S. suis serotype 18	960:978	This led to the identification of homologues of UDP-QuiNAc4NAc synthesis genes in S. suis serotype 18.					
30014879	4	80	theme	spectroscopic	507:519	arg1	data					521:524	Chemical and spectroscopic data	494:524	Chemical and spectroscopic data	494:524	Chemical and spectroscopic data gave the repeating unit sequence for serotype 3: [4)D-GlcA (β1-3)d-QuiNAc4NAc(β1-]n. To the best of our knowledge, this is the first report of di-N-acetyl-d-bacillosamine (QuiNAc4NAc) containing polysaccharides in Streptococci and the second time this rare diamino sugar has been observed in a Gram-positive bacterial species since its initial report.					
30194998	0	0	theme	pickle	95:100	arg1	cabbage					110:116	pickle Chinese cabbage	95:116	pickle Chinese cabbage	95:116	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	4	1	theme	purified	487:494	arg1	EPS					496:498	purified EPS	487:498	purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1	487:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	6	2	theme	in-vitro	948:955	arg1	activities					969:978	The in-vitro antioxidant activities	944:978	The in-vitro antioxidant activities of the dextran	944:993	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	6	3	theme	good	1002:1005	arg1	effects					1018:1024	good scavenging effects	1002:1024	good scavenging effects on superoxide anion radical and hydroxyl radical	1002:1073	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	4	4	theme	magnetic	569:576	arg1	resonance					578:586	nuclear magnetic resonance	561:586	nuclear magnetic resonance (NMR) spectra	561:600	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	4	theme	magnetic	569:576	arg1	NMR					589:591	NMR	589:591	NMR	589:591	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	6	5	theme	antioxidant	957:967	arg1	activities					969:978	The in-vitro antioxidant activities	944:978	The in-vitro antioxidant activities of the dextran	944:993	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	1	6	theme	Weissella	162:170	arg1	YB-1					180:183	Weissella cibaria YB-1	162:183	Weissella cibaria YB-1 isolated from pickle Chinese cabbage	162:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	4	7	theme	nuclear	561:567	arg1	resonance					578:586	nuclear magnetic resonance	561:586	nuclear magnetic resonance (NMR) spectra	561:600	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	7	theme	nuclear	561:567	arg1	NMR					589:591	NMR	589:591	NMR	589:591	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	8	dep	Fourier	514:520	arg1	transform					522:530	transform	522:530	transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1	522:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	9	theme	α-	734:735	arg1	branches					758:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	0	10	theme	Chinese	102:108	arg1	cabbage					110:116	pickle Chinese cabbage	95:116	pickle Chinese cabbage	95:116	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	4	11	theme	structural	456:465	arg1	characterization					467:482	The structural characterization	452:482	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1	452:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	5	12	theme	YB-1	864:867	arg1	dextran					869:875	YB-1 dextran	864:875	YB-1 dextran	864:875	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	4	13	theme	few	730:732	arg1	α-					734:735	only a few α-	723:735	only a few α-(1 → 3) (4.3%) linked branches	723:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	13	theme	few	730:732	arg1	%					748:748	4.3%	745:748	4.3%	745:748	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	13	theme	few	730:732	arg1	1 → 3					737:741	1 → 3	737:741	1 → 3	737:741	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	14	theme	resonance	578:586	arg1	spectra					594:600	nuclear magnetic resonance (NMR) spectra	561:600	nuclear magnetic resonance (NMR) spectra	561:600	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	5	15	theme	water	772:776	arg1	WSI					796:798	WSI	796:798	WSI	796:798	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	15	theme	water	772:776	arg1	index					789:793	The water solubility index	768:793	The water solubility index (WSI)	768:799	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	15	theme	water	772:776	arg1	%					927:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	index					789:793	The water solubility index	768:793	The water solubility index (WSI)	768:799	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	capacity					816:823	water holding capacity	802:823	water holding capacity (WHC)	802:829	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	%					927:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	activity					847:854	emulsifying activity	835:854	emulsifying activity (EA) of YB-1 dextran	835:875	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	EA					857:858	EA	857:858	EA	857:858	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	WSI					796:798	WSI	796:798	WSI	796:798	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	16	theme	dextran	869:875	arg1	WHC					826:828	WHC	826:828	WHC	826:828	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	3	17	theme	high	405:408	arg1	HPLC					445:448	HPLC	445:448	HPLC	445:448	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	17	theme	high	405:408	arg1	chromatography					429:442	high performance liquid chromatography	405:442	high performance liquid chromatography (HPLC)	405:449	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	1	18	theme	cibaria	172:178	arg1	YB-1					180:183	Weissella cibaria YB-1	162:183	Weissella cibaria YB-1 isolated from pickle Chinese cabbage	162:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	3	19	theme	gas	377:379	arg1	GC					397:398	GC	397:398	GC	397:398	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	19	theme	gas	377:379	arg1	chromatography					381:394	gas chromatography	377:394	gas chromatography (GC)	377:399	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	6	20	theme	dextran	987:993	arg1	activities					969:978	The in-vitro antioxidant activities	944:978	The in-vitro antioxidant activities of the dextran	944:993	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	4	21	link	linked	751:756	arg1	branches					758:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	3	22	theme	performance	410:420	arg1	HPLC					445:448	HPLC	445:448	HPLC	445:448	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	22	theme	performance	410:420	arg1	chromatography					429:442	high performance liquid chromatography	405:442	high performance liquid chromatography (HPLC)	405:449	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	4	23	theme	FT-IR	542:546	arg1	spectra					549:555	infrared (FT-IR) spectra	532:555	infrared (FT-IR) spectra	532:555	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	6	24	theme	hydroxyl	1058:1065	arg1	radical					1067:1073	hydroxyl radical	1058:1073	hydroxyl radical	1058:1073	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	4	25	theme	W.	620:621	arg1	YB-1					631:634	W. cibaria YB-1	620:634	W. cibaria YB-1	620:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	1	26	theme	Chinese	206:212	arg1	cabbage					214:220	pickle Chinese cabbage	199:220	pickle Chinese cabbage	199:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	3	27	theme	monosaccharide	267:280	arg1	glucose					309:315	glucose	309:315	glucose	309:315	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	27	theme	monosaccharide	267:280	arg1	composition					282:292	The monosaccharide composition	263:292	The monosaccharide composition of the EPS	263:303	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	4	28	theme	linked	751:756	arg1	branches					758:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	only a few α-(1 → 3) (4.3%) linked branches	723:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	29	theme	linear	650:655	arg1	dextran					657:663	a linear dextran	648:663	a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches	648:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	5	30	theme	solubility	778:787	arg1	WSI					796:798	WSI	796:798	WSI	796:798	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	30	theme	solubility	778:787	arg1	index					789:793	The water solubility index	768:793	The water solubility index (WSI)	768:799	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	30	theme	solubility	778:787	arg1	%					927:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%	882:927	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	3	31	theme	liquid	422:427	arg1	HPLC					445:448	HPLC	445:448	HPLC	445:448	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	31	theme	liquid	422:427	arg1	chromatography					429:442	high performance liquid chromatography	405:442	high performance liquid chromatography (HPLC)	405:449	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	0	32	theme	exopolysaccharide	37:53	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	0	32	theme	exopolysaccharide	37:53	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	4	33	theme	cibaria	623:629	arg1	YB-1					631:634	W. cibaria YB-1	620:634	W. cibaria YB-1	620:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	0	34	theme	Weissella	67:75	arg1	YB-1					85:88	Weissella cibaria YB-1	67:88	Weissella cibaria YB-1	67:88	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	1	35	attach	isolated	185:192	arg1	cabbage					214:220	pickle Chinese cabbage	199:220	pickle Chinese cabbage	199:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	1	35	attach	isolated	185:192	arg2	YB-1					180:183	Weissella cibaria YB-1	162:183	Weissella cibaria YB-1 isolated from pickle Chinese cabbage	162:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	4	36	theme	EPS	496:498	arg1	characterization					467:482	The structural characterization	452:482	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1	452:634	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	6	37	theme	anion	1040:1044	arg1	radical					1046:1052	superoxide anion radical	1029:1052	superoxide anion radical	1029:1052	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	4	38	theme	infrared	532:539	arg1	spectra					549:555	infrared (FT-IR) spectra	532:555	infrared (FT-IR) spectra	532:555	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	3	39	theme	EPS	301:303	arg1	glucose					309:315	glucose	309:315	glucose	309:315	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	3	39	theme	EPS	301:303	arg1	composition					282:292	The monosaccharide composition	263:292	The monosaccharide composition of the EPS	263:303	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	4	40	theme	glycosidic	698:707	arg1	linkages					709:716	α-(1 → 6) glycosidic linkages	688:716	α-(1 → 6) glycosidic linkages	688:716	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	5	41	theme	emulsifying	835:845	arg1	EA					857:858	EA	857:858	EA	857:858	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	41	theme	emulsifying	835:845	arg1	activity					847:854	emulsifying activity	835:854	emulsifying activity (EA) of YB-1 dextran	835:875	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	6	42	theme	superoxide	1029:1038	arg1	radical					1046:1052	superoxide anion radical	1029:1052	superoxide anion radical	1029:1052	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	3	43	theme	molecular	326:334	arg1	mass					336:339	its molecular mass	322:339	its molecular mass	322:339	The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC).					
30194998	6	44	theme	scavenging	1007:1016	arg1	effects					1018:1024	good scavenging effects	1002:1024	good scavenging effects on superoxide anion radical and hydroxyl radical	1002:1073	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	1	45	theme	pickle	199:204	arg1	cabbage					214:220	pickle Chinese cabbage	199:220	pickle Chinese cabbage	199:220	An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage.					
30194998	5	46	theme	water	802:806	arg1	capacity					816:823	water holding capacity	802:823	water holding capacity (WHC)	802:829	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	46	theme	water	802:806	arg1	WHC					826:828	WHC	826:828	WHC	826:828	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	0	47	theme	cibaria	77:83	arg1	YB-1					85:88	Weissella cibaria YB-1	67:88	Weissella cibaria YB-1	67:88	Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.					
30194998	4	48	contain	had	684:686	arg2	linkages					709:716	α-(1 → 6) glycosidic linkages	688:716	α-(1 → 6) glycosidic linkages	688:716	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	4	48	contain	had	684:686	arg1	dextran					657:663	a linear dextran	648:663	a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches	648:765	The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches.					
30194998	6	49	from	effects	1018:1024	arg1	radical					1067:1073	hydroxyl radical	1058:1073	hydroxyl radical	1058:1073	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	6	49	from	effects	1018:1024	arg1	radical					1046:1052	superoxide anion radical	1029:1052	superoxide anion radical	1029:1052	The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.					
30194998	5	50	theme	holding	808:814	arg1	capacity					816:823	water holding capacity	802:823	water holding capacity (WHC)	802:829	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30194998	5	50	theme	holding	808:814	arg1	WHC					826:828	WHC	826:828	WHC	826:828	The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively.					
30742969	0	0	theme	polysaccharide	108:121	arg1	Characterization					0:15	Characterization	0:15	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.	0:122	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	2	1	theme	UDP-GlcA	518:525	arg1	gene					541:544	the UDP-GlcA decarboxylase gene	514:544	the UDP-GlcA decarboxylase gene	514:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	7	2	theme	2OHC24:0	1299:1306	arg1	acid					1314:1317	2OHC24:0 fatty acid	1299:1317	2OHC24:0 fatty acid	1299:1317	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	2	theme	2OHC24:0	1299:1306	arg1	acid					1293:1296	lignoceric acid	1282:1296	C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1240:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	0	3	theme	wall	103:106	arg1	polysaccharide					108:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	5	4	with	O-3	946:948	arg1	-β-Gal-					1004:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	3	5	theme	Monosaccharide	547:560	arg1	composition					562:572	Monosaccharide composition	547:572	Monosaccharide composition	547:572	Monosaccharide composition and methylation analyses, as well as nuclear magnetic resonance spectroscopy were employed in discerning the structure.					
30742969	7	6	theme	C18-phytosphingosine	1240:1259	arg1	acid					1314:1317	2OHC24:0 fatty acid	1299:1317	2OHC24:0 fatty acid	1299:1317	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	6	theme	C18-phytosphingosine	1240:1259	arg1	acid					1293:1296	lignoceric acid	1282:1296	C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1240:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	4	7	theme	mannose	823:829	arg1	residues					831:838	xylose and 2-O-acetylated mannose residues	797:838	xylose and 2-O-acetylated mannose residues	797:838	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	4	8	theme	xylose	797:802	arg1	residues					831:838	xylose and 2-O-acetylated mannose residues	797:838	xylose and 2-O-acetylated mannose residues	797:838	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	7	9	theme	glycolipid	1152:1161	arg1	anchor					1163:1168	the glycolipid anchor	1148:1168	the glycolipid anchor of the LGMGal	1148:1182	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	10	theme	fatty	1308:1312	arg1	acid					1314:1317	2OHC24:0 fatty acid	1299:1317	2OHC24:0 fatty acid	1299:1317	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	10	theme	fatty	1308:1312	arg1	acid					1293:1296	lignoceric acid	1282:1296	C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1240:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	4	11	theme	2-O-acetylated	808:821	arg1	residues					831:838	xylose and 2-O-acetylated mannose residues	797:838	xylose and 2-O-acetylated mannose residues	797:838	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	2	12	theme	first	372:376	arg1	time					378:381	the first time	368:381	the first time	368:381	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	5	13	theme	galactan	862:869	arg1	chain					876:880	a galactan main chain -[-6-α-Gal-]-	860:894	a galactan main chain -[-6-α-Gal-]-	860:894	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	3	14	theme	methylation	578:588	arg1	analyses					590:597	methylation analyses	578:597	methylation analyses	578:597	Monosaccharide composition and methylation analyses, as well as nuclear magnetic resonance spectroscopy were employed in discerning the structure.					
30742969	5	15	theme	oligosaccharide	958:972	arg1	-β-Gal-					1004:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	7	16	theme	LGMGal	1177:1182	arg1	anchor					1163:1168	the glycolipid anchor	1148:1168	the glycolipid anchor of the LGMGal	1148:1182	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	2	17	contain	carrying	491:498	arg1	neoformans					480:489	C. neoformans	477:489	a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene	468:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	2	17	contain	carrying	491:498	arg2	deletion					502:509	a deletion	500:509	a deletion in the UDP-GlcA decarboxylase gene	500:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	4	18	theme	residues	831:838	arg1	absence					786:792	the absence	782:792	the absence of xylose and 2-O-acetylated mannose residues	782:838	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	5	19	theme	second	909:914	arg1	residue					920:926	every second Gal residue	903:926	every second Gal residue	903:926	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	2	20	theme	neoformans	480:489	arg1	surface					457:463	the surface	453:463	the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene	453:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	2	21	from	deletion	502:509	arg1	gene					541:544	the UDP-GlcA decarboxylase gene	514:544	the UDP-GlcA decarboxylase gene	514:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	7	22	theme	lignoceric	1282:1291	arg1	acid					1314:1317	2OHC24:0 fatty acid	1299:1317	2OHC24:0 fatty acid	1299:1317	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	22	theme	lignoceric	1282:1291	arg1	acid					1293:1296	lignoceric acid	1282:1296	C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1240:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	3	23	theme	nuclear	611:617	arg1	resonance					628:636	nuclear magnetic resonance	611:636	nuclear magnetic resonance spectroscopy	611:649	Monosaccharide composition and methylation analyses, as well as nuclear magnetic resonance spectroscopy were employed in discerning the structure.					
30742969	7	24	theme	myo-inositol	1199:1210	arg1	phosphoceramide					1212:1226	an myo-inositol phosphoceramide	1196:1226	an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1196:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	5	25	theme	Gal	916:918	arg1	residue					920:926	every second Gal residue	903:926	every second Gal residue	903:926	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	5	26	theme	main	871:874	arg1	chain					876:880	a galactan main chain -[-6-α-Gal-]-	860:894	a galactan main chain -[-6-α-Gal-]-	860:894	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	1	27	theme	host	328:331	arg1	response					340:347	the host immune response	324:347	the host immune response	324:347	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	3	28	theme	magnetic	619:626	arg1	resonance					628:636	nuclear magnetic resonance	611:636	nuclear magnetic resonance spectroscopy	611:649	Monosaccharide composition and methylation analyses, as well as nuclear magnetic resonance spectroscopy were employed in discerning the structure.					
30742969	4	29	theme	polysaccharide	720:733	arg1	structure					735:743	the polysaccharide structure	716:743	the polysaccharide structure of the LGMGal	716:757	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	0	30	theme	6-O-acetylated	24:37	arg1	neoformans					87:96	6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans	24:96	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	1	31	theme	immune	333:338	arg1	response					340:347	the host immune response	324:347	the host immune response	324:347	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	5	32	theme	α-Man6OAc-3-α-Man-4-	974:993	arg1	-β-Gal-					1004:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	5	33	theme	β-GlcA-3	995:1002	arg1	-β-Gal-					1004:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-	955:1010	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	1	34	theme	response	340:347	arg1	impairment					310:319	impairment	310:319	impairment of the host immune response	310:347	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	4	35	theme	LGMGal	752:757	arg1	structure					735:743	the polysaccharide structure	716:743	the polysaccharide structure of the LGMGal	716:757	Our results show that the polysaccharide structure of the LGMGal differs from GXMGal by the absence of xylose and 2-O-acetylated mannose residues.					
30742969	0	36	theme	a	66:66	arg1	neoformans					87:96	6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans	24:96	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	0	37	theme	lipoglucuronomannogalactan	39:64	arg1	neoformans					87:96	6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans	24:96	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	6	38	theme	units	1092:1096	arg1	rate					1077:1080	The substitution rate	1060:1080	The substitution rate of β-Galp units by GlcpA	1060:1105	The substitution rate of β-Galp units by GlcpA is 35%.					
30742969	6	38	theme	units	1092:1096	arg1	%					1112:1112	35%	1110:1112	35%	1110:1112	The substitution rate of β-Galp units by GlcpA is 35%.					
30742969	0	39	theme	Cryptococcus	74:85	arg1	neoformans					87:96	6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans	24:96	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	2	40	theme	decarboxylase	527:539	arg1	gene					541:544	the UDP-GlcA decarboxylase gene	514:544	the UDP-GlcA decarboxylase gene	514:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	3	41	theme	resonance	628:636	arg1	spectroscopy					638:649	nuclear magnetic resonance spectroscopy	611:649	nuclear magnetic resonance spectroscopy	611:649	Monosaccharide composition and methylation analyses, as well as nuclear magnetic resonance spectroscopy were employed in discerning the structure.					
30742969	0	42	theme	novel	68:72	arg1	neoformans					87:96	6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans	24:96	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	2	43	attach	isolated	439:446	arg2	LGMGal					430:435	LGMGal	430:435	LGMGal	430:435	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	2	43	attach	isolated	439:446	arg2	lipoglucuronomannogalactan					402:427	a lipoglucuronomannogalactan	400:427	a lipoglucuronomannogalactan (LGMGal)	400:436	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	2	43	attach	isolated	439:446	arg1	surface					457:463	the surface	453:463	the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene	453:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	1	44	theme	characteristic	166:179	arg1	Glucuronoxylomannogalactans					124:150	Glucuronoxylomannogalactans	124:150	Glucuronoxylomannogalactans (GXMGals)	124:160	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	1	44	theme	characteristic	166:179	arg1	polysaccharides					190:204	characteristic capsular polysaccharides	166:204	characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response	166:347	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	6	45	theme	β-Galp	1085:1090	arg1	units					1092:1096	β-Galp units	1085:1096	β-Galp units	1085:1096	The substitution rate of β-Galp units by GlcpA is 35%.					
30742969	7	46	theme	monohydroxylated	1265:1280	arg1	acid					1314:1317	2OHC24:0 fatty acid	1299:1317	2OHC24:0 fatty acid	1299:1317	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	7	46	theme	monohydroxylated	1265:1280	arg1	acid					1293:1296	lignoceric acid	1282:1296	C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid)	1240:1318	Additionally, we determined that the glycolipid anchor of the LGMGal is based on an myo-inositol phosphoceramide composed of C18-phytosphingosine and monohydroxylated lignoceric acid (2OHC24:0 fatty acid).					
30742969	1	47	theme	capsular	181:188	arg1	Glucuronoxylomannogalactans					124:150	Glucuronoxylomannogalactans	124:150	Glucuronoxylomannogalactans (GXMGals)	124:160	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	1	47	theme	capsular	181:188	arg1	polysaccharides					190:204	characteristic capsular polysaccharides	166:204	characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response	166:347	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	1	48	theme	cryptococcal	282:293	arg1	virulence					295:303	cryptococcal virulence	282:303	cryptococcal virulence	282:303	Glucuronoxylomannogalactans (GXMGals) are characteristic capsular polysaccharides produced by the opportunistic fungus C. neoformans, which are implicated in cryptococcal virulence, via impairment of the host immune response.					
30742969	0	49	theme	cell	98:101	arg1	polysaccharide					108:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	5	50	from	components	1013:1022	arg1	italic					1027:1032	italic	1027:1032	italic	1027:1032	LGMGal consists of a galactan main chain -[-6-α-Gal-]-, where every second Gal residue is substituted at O-3 with an oligosaccharide α-Man6OAc-3-α-Man-4-(β-GlcA-3)-β-Gal-; components in italic being non-stoichiometric.					
30742969	0	51	theme	neoformans	87:96	arg1	polysaccharide					108:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide	20:121	Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide.					
30742969	2	52	theme	lipoglucuronomannogalactan	402:427	arg1	structure					387:395	the structure	383:395	the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene	383:544	We determined for the first time the structure of a lipoglucuronomannogalactan (LGMGal), isolated from the surface of a mutant C. neoformans carrying a deletion in the UDP-GlcA decarboxylase gene.					
30742969	6	53	theme	substitution	1064:1075	arg1	rate					1077:1080	The substitution rate	1060:1080	The substitution rate of β-Galp units by GlcpA	1060:1105	The substitution rate of β-Galp units by GlcpA is 35%.					
30742969	6	53	theme	substitution	1064:1075	arg1	%					1112:1112	35%	1110:1112	35%	1110:1112	The substitution rate of β-Galp units by GlcpA is 35%.					
30439423	4	0	theme	separation	882:891	arg1	methods					893:899	conventional ethanol precipitation and separation methods	843:899	methods	893:899	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	3	1	theme	extraction	653:662	arg1	time					664:667	extraction time	653:667	extraction time	653:667	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	3	2	theme	%	638:638	arg1	reduction					640:648	~80% reduction	635:648	~80% reduction in extraction time	635:667	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	3	theme	EPS	342:344	arg1	%					355:355	52.09%	350:355	52.09%	350:355	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	3	theme	EPS	342:344	arg1	yield					333:337	The maximum extraction yield	310:337	The maximum extraction yield of EPS	310:344	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	7	4	theme	green	1288:1292	arg1	technique					1305:1313	a simple and green separation technique	1275:1313	a simple and green separation technique	1275:1313	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	0	5	theme	exopolysaccharides	79:96	arg1	extraction					40:49	direct extraction	33:49	direct extraction	33:49	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	0	5	theme	exopolysaccharides	79:96	arg1	separation					55:64	separation	55:64	separation	55:64	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	1	6	theme	three-phase	158:168	arg1	TPP					184:186	TPP	184:186	TPP	184:186	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	6	theme	three-phase	158:168	arg1	partitioning					170:181	three-phase partitioning	158:181	three-phase partitioning (TPP)	158:187	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	0	7	theme	cultured	107:114	arg1	broth					116:120	the cultured broth	103:120	the cultured broth of Phellinus baumii	103:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	3	8	theme	extraction	614:623	arg1	yield					625:629	extraction yield	614:629	extraction yield	614:629	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	0	9	dep	extraction	40:49	arg1	the					29:31	the	29:31	the	29:31	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	2	10	theme	cultured	462:469	arg1	broth					471:475	cultured broth	462:475	cultured broth	462:475	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	0	11	from	separation	55:64	arg1	broth					116:120	the cultured broth	103:120	the cultured broth of Phellinus baumii	103:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	4	12	theme	conventional	843:854	arg1	precipitation					864:876	conventional ethanol precipitation and separation methods	843:899	precipitation	864:876	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	7	13	theme	mushrooms	1408:1416	arg1	broths					1398:1403	the fermentation broths	1381:1403	the fermentation broths of mushrooms and other fungi	1381:1432	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	4	14	theme	acid	746:749	arg1	contents					759:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents	709:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS	709:792	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	4	14	theme	acid	746:749	arg1	higher					799:804	higher	799:804	higher	799:804	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	7	15	theme	separate	1353:1360	arg1	EPS					1372:1374	separate bioactive EPS	1353:1374	separate bioactive EPS	1353:1374	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	7	16	theme	bioactive	1362:1370	arg1	EPS					1372:1374	separate bioactive EPS	1353:1374	separate bioactive EPS	1353:1374	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	2	17	theme	following	367:375	arg1	35 °C					503:507	35 °C	503:507	35 °C	503:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	17	theme	following	367:375	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	17	theme	following	367:375	arg1	30 min					491:496	30 min	491:496	30 min	491:496	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	17	theme	following	367:375	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	17	theme	following	367:375	arg1	ratio					453:457	1.0:1.5 (v/v) ratio	439:457	1.0:1.5 (v/v) ratio of cultured broth to t‑butanol	439:488	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	0	18	from	extraction	40:49	arg1	broth					116:120	the cultured broth	103:120	the cultured broth of Phellinus baumii	103:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	1	19	from	broth	283:287	arg1	extract					210:216	extract	210:216	extract	210:216	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	19	from	broth	283:287	arg1	EPSs					261:264	EPSs	261:264	EPSs	261:264	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	19	from	broth	283:287	arg1	exopolysaccharides					241:258	separate bioactive exopolysaccharides	222:258	separate bioactive exopolysaccharides (EPSs)	222:265	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	6	20	theme	inhibitory	1124:1133	arg1	activities					1135:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	7	21	theme	other	1422:1426	arg1	fungi					1428:1432	other fungi	1422:1432	other fungi	1422:1432	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	2	22	theme	1.0:1.5	439:445	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	22	theme	1.0:1.5	439:445	arg1	ratio					453:457	1.0:1.5 (v/v) ratio	439:457	1.0:1.5 (v/v) ratio of cultured broth to t‑butanol	439:488	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	6	23	theme	α‑glycosidase	1110:1122	arg1	activities					1135:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	0	24	theme	Three-phase	0:10	arg1	partitioning					12:23	Three-phase partitioning	0:23	Three-phase partitioning	0:23	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	5	25	theme	different	977:985	arg1	compositions					1002:1013	different monosaccharide compositions	977:1013	different monosaccharide compositions	977:1013	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	2	26	theme	broth	471:475	arg1	35 °C					503:507	35 °C	503:507	35 °C	503:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	26	theme	broth	471:475	arg1	30 min					491:496	30 min	491:496	30 min	491:496	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	26	theme	broth	471:475	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	26	theme	broth	471:475	arg1	ratio					453:457	1.0:1.5 (v/v) ratio	439:457	1.0:1.5 (v/v) ratio of cultured broth to t‑butanol	439:488	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	26	theme	broth	471:475	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	5	27	theme	structural	946:955	arg1	characteristics					957:971	similar preliminary structural characteristics	926:971	similar preliminary structural characteristics	926:971	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	7	28	theme	fermentation	1385:1396	arg1	broths					1398:1403	the fermentation broths	1381:1403	the fermentation broths of mushrooms and other fungi	1381:1432	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	3	29	from	reduction	640:648	arg1	yield					625:629	extraction yield	614:629	extraction yield	614:629	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	3	29	from	reduction	640:648	arg1	time					664:667	extraction time	653:667	extraction time	653:667	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	6	30	theme	stimulation	1162:1172	arg1	activities					1174:1183	macrophage stimulation activities	1151:1183	macrophage stimulation activities	1151:1183	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	0	31	theme	baumii	135:140	arg1	broth					116:120	the cultured broth	103:120	the cultured broth of Phellinus baumii	103:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	3	32	theme	multifrequency	512:525	arg1	ultrasound					533:542	A multifrequency power ultrasound	510:542	A multifrequency power ultrasound in a sequential mode coupled with TPP	510:580	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	6	33	theme	α‑amylase	1096:1104	arg1	activities					1135:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	3	34	theme	power	527:531	arg1	ultrasound					533:542	A multifrequency power ultrasound	510:542	A multifrequency power ultrasound in a sequential mode coupled with TPP	510:580	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	35	theme	20	397:398	arg1	%					399:399	%	399:399	%	399:399	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	36	theme	%	399:399	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	36	theme	%	399:399	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	1	37	theme	cultured	274:281	arg1	broth					283:287	a cultured broth	272:287	a cultured broth of Phellinus baumii	272:307	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	0	38	theme	Phellinus	125:133	arg1	baumii					135:140	Phellinus baumii	125:140	Phellinus baumii	125:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	5	39	theme	monosaccharide	987:1000	arg1	compositions					1002:1013	different monosaccharide compositions	977:1013	different monosaccharide compositions	977:1013	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	5	40	theme	similar	926:932	arg1	characteristics					957:971	similar preliminary structural characteristics	926:971	similar preliminary structural characteristics	926:971	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	6	41	theme	antioxidant	1072:1082	arg1	capacities					1084:1093	antioxidant capacities	1072:1093	antioxidant capacities	1072:1093	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	3	42	from	increment	601:609	arg1	yield					625:629	extraction yield	614:629	extraction yield	614:629	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	3	42	from	increment	601:609	arg1	time					664:667	extraction time	653:667	extraction time	653:667	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	43	dep	conditions	385:394	arg1	35 °C					503:507	35 °C	503:507	35 °C	503:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	43	dep	conditions	385:394	arg1	30 min					491:496	30 min	491:496	30 min	491:496	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	43	dep	conditions	385:394	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	43	dep	conditions	385:394	arg1	ratio					453:457	1.0:1.5 (v/v) ratio	439:457	1.0:1.5 (v/v) ratio of cultured broth to t‑butanol	439:488	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	43	dep	conditions	385:394	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	3	44	from	ultrasound	533:542	arg1	mode					560:563	a sequential mode	547:563	a sequential mode coupled with TPP	547:580	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	5	45	theme	preliminary	934:944	arg1	characteristics					957:971	similar preliminary structural characteristics	926:971	similar preliminary structural characteristics	926:971	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	0	46	theme	direct	33:38	arg1	extraction					40:49	direct extraction	33:49	direct extraction	33:49	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	3	47	theme	traditional	692:702	arg1	TPP					704:706	traditional TPP	692:706	traditional TPP	692:706	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	48	theme	optimal	377:383	arg1	35 °C					503:507	35 °C	503:507	35 °C	503:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	48	theme	optimal	377:383	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	48	theme	optimal	377:383	arg1	30 min					491:496	30 min	491:496	30 min	491:496	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	48	theme	optimal	377:383	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	48	theme	optimal	377:383	arg1	ratio					453:457	1.0:1.5 (v/v) ratio	439:457	1.0:1.5 (v/v) ratio of cultured broth to t‑butanol	439:488	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	49	theme	sulfate	416:422	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	49	theme	sulfate	416:422	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	1	50	theme	Phellinus	292:300	arg1	baumii					302:307	Phellinus baumii	292:307	Phellinus baumii	292:307	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	4	51	theme	purified	781:788	arg1	EPS					790:792	partially purified EPS	771:792	partially purified EPS	771:792	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	5	52	theme	molecular	1019:1027	arg1	weights					1029:1035	molecular weights	1019:1035	molecular weights	1019:1035	EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights.					
30439423	1	53	used	used	193:196	arg2	partitioning					170:181	three-phase partitioning	158:181	three-phase partitioning (TPP)	158:187	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	53	used	used	193:196	arg2	TPP					184:186	TPP	184:186	TPP	184:186	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	3	54	theme	~80	635:637	arg1	%					638:638	%	638:638	%	638:638	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	55	theme	ammonium	407:414	arg1	conditions					385:394	the following optimal conditions	363:394	the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C	363:507	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	55	theme	ammonium	407:414	arg1	concentration					424:436	20% (w/v) ammonium sulfate concentration	397:436	20% (w/v) ammonium sulfate concentration	397:436	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	1	56	theme	baumii	302:307	arg1	broth					283:287	a cultured broth	272:287	a cultured broth of Phellinus baumii	272:307	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	3	57	theme	%	599:599	arg1	increment					601:609	~9.12% increment	594:609	~9.12% increment in extraction yield	594:629	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	58	dep	%	399:399	arg1	w/v					402:404	w/v	402:404	w/v	402:404	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	59	theme	extraction	322:331	arg1	%					355:355	52.09%	350:355	52.09%	350:355	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	59	theme	extraction	322:331	arg1	yield					333:337	The maximum extraction yield	310:337	The maximum extraction yield of EPS	310:344	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	6	60	theme	EPS	1188:1190	arg1	activities					1174:1183	macrophage stimulation activities	1151:1183	macrophage stimulation activities	1151:1183	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	6	60	theme	EPS	1188:1190	arg1	activities					1135:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	α‑amylase and α‑glycosidase inhibitory activities	1096:1144	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	6	60	theme	EPS	1188:1190	arg1	higher					1202:1207	higher	1202:1207	higher	1202:1207	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	6	60	theme	EPS	1188:1190	arg1	abilities					1061:1069	The radical-scavenging abilities	1038:1069	The radical-scavenging abilities	1038:1069	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	6	60	theme	EPS	1188:1190	arg1	capacities					1084:1093	antioxidant capacities	1072:1093	antioxidant capacities	1072:1093	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	4	61	theme	carbohydrate	713:724	arg1	contents					759:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents	709:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS	709:792	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	4	61	theme	carbohydrate	713:724	arg1	higher					799:804	higher	799:804	higher	799:804	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	0	62	from	broth	116:120	arg1	exopolysaccharides					79:96	bioactive exopolysaccharides	69:96	bioactive exopolysaccharides from the cultured broth of Phellinus baumii	69:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	0	62	from	broth	116:120	arg1	extraction					40:49	direct extraction	33:49	direct extraction	33:49	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	0	62	from	broth	116:120	arg1	separation					55:64	separation	55:64	separation	55:64	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	3	63	theme	sequential	549:558	arg1	mode					560:563	a sequential mode	547:563	a sequential mode coupled with TPP	547:580	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	2	64	theme	maximum	314:320	arg1	%					355:355	52.09%	350:355	52.09%	350:355	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	2	64	theme	maximum	314:320	arg1	yield					333:337	The maximum extraction yield	310:337	The maximum extraction yield of EPS	310:344	The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C.					
30439423	4	65	theme	uronic	739:744	arg1	acid					746:749	uronic acid	739:749	uronic acid (3.37%)	739:757	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	4	65	theme	uronic	739:744	arg1	%					756:756	3.37%	752:756	3.37%	752:756	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	4	66	theme	EPS	790:792	arg1	contents					759:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents	709:766	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS	709:792	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	4	66	theme	EPS	790:792	arg1	higher					799:804	higher	799:804	higher	799:804	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	7	67	theme	separation	1294:1303	arg1	technique					1305:1313	a simple and green separation technique	1275:1313	a simple and green separation technique	1275:1313	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	7	68	used	used	1324:1327	arg2	TPP					1268:1270	TPP	1268:1270	TPP as a simple and green separation technique	1268:1313	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	6	69	theme	macrophage	1151:1160	arg1	activities					1174:1183	macrophage stimulation activities	1151:1183	macrophage stimulation activities	1151:1183	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	0	70	theme	bioactive	69:77	arg1	exopolysaccharides					79:96	bioactive exopolysaccharides	69:96	bioactive exopolysaccharides from the cultured broth of Phellinus baumii	69:140	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	3	71	theme	~9.12	594:598	arg1	%					599:599	%	599:599	%	599:599	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	0	72	dep	partitioning	12:23	arg1	extraction					40:49	direct extraction	33:49	direct extraction	33:49	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	0	72	dep	partitioning	12:23	arg1	separation					55:64	separation	55:64	separation	55:64	Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii.					
30439423	6	73	theme	radical-scavenging	1042:1059	arg1	abilities					1061:1069	The radical-scavenging abilities	1038:1069	The radical-scavenging abilities	1038:1069	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	6	73	theme	radical-scavenging	1042:1059	arg1	higher					1202:1207	higher	1202:1207	higher	1202:1207	The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C.					
30439423	7	74	theme	simple	1277:1282	arg1	technique					1305:1313	a simple and green separation technique	1275:1313	a simple and green separation technique	1275:1313	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	1	75	theme	separate	222:229	arg1	EPSs					261:264	EPSs	261:264	EPSs	261:264	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	75	theme	separate	222:229	arg1	exopolysaccharides					241:258	separate bioactive exopolysaccharides	222:258	separate bioactive exopolysaccharides (EPSs)	222:265	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	4	76	theme	ethanol	856:862	arg1	precipitation					864:876	conventional ethanol precipitation and separation methods	843:899	precipitation	864:876	The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods.					
30439423	7	77	theme	fungi	1428:1432	arg1	broths					1398:1403	the fermentation broths	1381:1403	the fermentation broths of mushrooms and other fungi	1381:1432	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	3	78	dep	resulted	582:589	arg1	compared					669:676	compared	669:676	compared with those of traditional TPP	669:706	A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP.					
30439423	7	79	from	broths	1398:1403	arg1	EPS					1372:1374	separate bioactive EPS	1353:1374	separate bioactive EPS	1353:1374	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	7	79	from	broths	1398:1403	arg1	extract					1341:1347	extract	1341:1347	extract	1341:1347	Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi.					
30439423	1	80	theme	bioactive	231:239	arg1	EPSs					261:264	EPSs	261:264	EPSs	261:264	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
30439423	1	80	theme	bioactive	231:239	arg1	exopolysaccharides					241:258	separate bioactive exopolysaccharides	222:258	separate bioactive exopolysaccharides (EPSs)	222:265	In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii.					
31734359	4	0	theme	SeH	1037:1039	arg1	bond					1041:1044	the SeH bond	1033:1044	the SeH bond on the branch of the polysaccharide	1033:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	1	theme	I2-KI	1169:1173	arg1	reaction					1175:1182	I2-KI reaction	1169:1182	I2-KI reaction	1169:1182	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	4	2	theme	selenyl	908:914	arg1	ester					916:920	selenyl ester	908:920	selenyl ester	908:920	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	7	3	from	difference	1487:1496	arg1	methods					1525:1531	artificial selenization methods	1501:1531	artificial selenization methods	1501:1531	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	0	4	theme	green	139:143	arg1	tea					145:147	synthetic selenized green tea	119:147	synthetic selenized green tea	119:147	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	7	5	contain	had	1651:1653	arg2	effect					1669:1674	a significant effect	1655:1674	a significant effect	1655:1674	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	7	5	contain	had	1651:1653	arg1	difference					1487:1496	the difference	1483:1496	the difference in artificial selenization methods	1483:1531	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	3	6	theme	different	632:640	arg1	composition					657:667	different monosaccharide composition	632:667	different monosaccharide composition	632:667	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	4	7	from	position	860:867	arg1	polysaccharide					876:889	the polysaccharide	872:889	the polysaccharide	872:889	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	7	from	position	860:867	arg1	group					843:847	the hydroxyl group	830:847	the hydroxyl group at the C-6 position in the polysaccharide	830:889	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	8	theme	Congo	1153:1157	arg1	test					1163:1166	the Congo red test	1149:1166	the Congo red test	1149:1166	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	7	9	theme	different	1574:1582	arg1	characteristics					1595:1609	different structural characteristics	1574:1609	different structural characteristics	1574:1609	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	3	10	theme	molar	670:674	arg1	ratio					676:680	molar ratio	670:680	molar ratio	670:680	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	4	11	from	branch	1053:1058	arg1	form					1025:1028	the form	1021:1028	the form of the SeH bond on the branch of the polysaccharide	1021:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	12	theme	artificial	1234:1243	arg1	Se-TPSs					1245:1251	the two artificial Se-TPSs	1226:1251	the two artificial Se-TPSs	1226:1251	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	6	13	theme	significant	1321:1331	arg1	differences					1333:1343	significant differences	1321:1343	significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity	1321:1451	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	1	14	theme	recent	169:174	arg1	years					176:180	recent years	169:180	recent years	169:180	In recent years, selenium-enriched polysaccharides (Se-PS) have been paid more and more attention, and the activity of many Se-PSs has been studied but little on their structure.					
31734359	4	15	theme	C-1	997:999	arg1	position					1009:1016	the C-1 and C-6 position	993:1016	position	1009:1016	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	2	16	from	forms	431:435	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	16	from	forms	431:435	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	4	17	theme	Raman	748:752	arg1	spectra					754:760	Raman spectra	748:760	Raman spectra	748:760	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	18	theme	XRD	1201:1203	arg1	analysis					1205:1212	XRD analysis	1201:1212	XRD analysis	1201:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	19	theme	studies	1104:1110	arg1	series					1094:1099	a series	1092:1099	a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1092:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	3	20	with	heteropolysaccharides	605:625	arg1	composition					657:667	different monosaccharide composition	632:667	different monosaccharide composition	632:667	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	3	20	with	heteropolysaccharides	605:625	arg1	ratio					676:680	molar ratio	670:680	molar ratio	670:680	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	3	20	with	heteropolysaccharides	605:625	arg1	content					696:702	selenium content	687:702	selenium content	687:702	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	4	21	from	selenium	800:807	arg1	CSe-tps1					812:819	CSe-tps1	812:819	CSe-tps1	812:819	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	7	22	theme	selenization	1512:1523	arg1	methods					1525:1531	artificial selenization methods	1501:1531	artificial selenization methods	1501:1531	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	4	23	theme	hydroxyl	975:982	arg1	group					984:988	the hydroxyl group	971:988	the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide	971:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	0	24	from	selenium	43:50	arg1	tea					145:147	synthetic selenized green tea	119:147	synthetic selenized green tea	119:147	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	0	24	from	selenium	43:50	arg1	selenium-enriched					97:113	selenium-enriched	97:113	selenium-enriched	97:113	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	0	25	theme	synthetic	119:127	arg1	tea					145:147	synthetic selenized green tea	119:147	synthetic selenized green tea	119:147	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	2	26	theme	selenium-enriched	477:493	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	26	theme	selenium-enriched	477:493	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	0	27	dep	selenium-enriched	97:113	arg1	polysaccharides					149:163	polysaccharides	149:163	polysaccharides	149:163	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	4	28	theme	NMR	773:775	arg1	analysis					777:784	2D NMR analysis	770:784	2D NMR analysis	770:784	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	28	theme	NMR	773:775	arg1	1D					766:767	1D	766:767	1D	766:767	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	29	theme	more	1286:1289	arg1	branches					1291:1298	more branches	1286:1298	more branches	1286:1298	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	0	30	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of existence	0:32	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	2	31	theme	present	423:429	arg1	forms					431:435	present forms	423:435	present forms	423:435	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	6	32	theme	apparent	1368:1375	arg1	morphology					1377:1386	apparent morphology	1368:1386	apparent morphology	1368:1386	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	4	33	from	form	1025:1028	arg1	branch					1053:1058	the branch	1049:1058	the branch of the polysaccharide	1049:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	34	theme	C-6	1005:1007	arg1	position					1009:1016	the C-1 and C-6 position	993:1016	position	1009:1016	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	3	35	theme	selenium	687:694	arg1	content					696:702	selenium content	687:702	selenium content	687:702	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	5	36	theme	triple	1259:1264	arg1	structure					1272:1280	a triple helix structure	1257:1280	a triple helix structure	1257:1280	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	2	37	theme	different	456:464	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	37	theme	different	456:464	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	0	38	theme	structural	56:65	arg1	characteristics					67:81	structural characteristics	56:81	structural characteristics	56:81	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	6	39	theme	inhibition	1433:1442	arg1	activity					1444:1451	the α-glucosidase inhibition activity	1415:1451	the α-glucosidase inhibition activity	1415:1451	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	5	40	theme	FESEM	1185:1189	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	2	41	from	characterization	402:417	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	41	from	characterization	402:417	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	4	42	theme	polysaccharide	1067:1080	arg1	branch					1053:1058	the branch	1049:1058	the branch of the polysaccharide	1049:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	43	theme	DSC	1192:1194	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	44	from	series	1094:1099	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	4	45	theme	bond	1041:1044	arg1	form					1025:1028	the form	1021:1028	the form of the SeH bond on the branch of the polysaccharide	1021:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	46	theme	structural	1119:1128	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	47	from	characteristics	1130:1144	arg1	series					1094:1099	a series	1092:1099	a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1092:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	6	48	theme	release	1394:1400	arg1	ability					1402:1408	heat release ability	1389:1408	heat release ability	1389:1408	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	5	49	theme	reaction	1175:1182	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	7	50	from	form	1683:1686	arg1	polysaccharides					1703:1717	polysaccharides	1703:1717	polysaccharides	1703:1717	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	1	51	theme	selenium-enriched	183:199	arg1	Se-PS					218:222	Se-PS	218:222	Se-PS	218:222	In recent years, selenium-enriched polysaccharides (Se-PS) have been paid more and more attention, and the activity of many Se-PSs has been studied but little on their structure.					
31734359	1	51	theme	selenium-enriched	183:199	arg1	polysaccharides					201:215	selenium-enriched polysaccharides	183:215	selenium-enriched polysaccharides (Se-PS)	183:223	In recent years, selenium-enriched polysaccharides (Se-PS) have been paid more and more attention, and the activity of many Se-PSs has been studied but little on their structure.					
31734359	1	52	theme	Se-PSs	290:295	arg1	activity					273:280	the activity	269:280	the activity of many Se-PSs	269:295	In recent years, selenium-enriched polysaccharides (Se-PS) have been paid more and more attention, and the activity of many Se-PSs has been studied but little on their structure.					
31734359	7	53	from	selenium	1691:1698	arg1	polysaccharides					1703:1717	polysaccharides	1703:1717	polysaccharides	1703:1717	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	5	54	theme	red	1159:1161	arg1	test					1163:1166	the Congo red test	1149:1166	the Congo red test	1149:1166	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	6	55	from	differences	1333:1343	arg1	morphology					1356:1365	crystal morphology	1348:1365	crystal morphology	1348:1365	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	6	55	from	differences	1333:1343	arg1	morphology					1377:1386	apparent morphology	1368:1386	apparent morphology	1368:1386	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	6	55	from	differences	1333:1343	arg1	ability					1402:1408	heat release ability	1389:1408	heat release ability	1389:1408	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	6	55	from	differences	1333:1343	arg1	activity					1444:1451	the α-glucosidase inhibition activity	1415:1451	the α-glucosidase inhibition activity	1415:1451	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	7	56	theme	hypoglycemic	1619:1630	arg1	activity					1632:1639	the hypoglycemic activity	1615:1639	the hypoglycemic activity	1615:1639	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	7	57	from	polysaccharides	1703:1717	arg1	form					1683:1686	the form	1679:1686	the form of selenium in polysaccharides	1679:1717	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	4	58	theme	Structural	705:714	arg1	1D					766:767	1D	766:767	1D	766:767	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	58	theme	Structural	705:714	arg1	FT-IR					741:745	FT-IR	741:745	FT-IR	741:745	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	58	theme	Structural	705:714	arg1	spectra					754:760	Raman spectra	748:760	Raman spectra	748:760	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	58	theme	Structural	705:714	arg1	investigations					716:729	Structural investigations	705:729	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis	705:784	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	5	59	contain	had	1253:1255	arg2	branches					1291:1298	more branches	1286:1298	more branches	1286:1298	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	59	contain	had	1253:1255	arg1	Se-TPSs					1245:1251	the two artificial Se-TPSs	1226:1251	the two artificial Se-TPSs	1226:1251	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	59	contain	had	1253:1255	arg2	structure					1272:1280	a triple helix structure	1257:1280	a triple helix structure	1257:1280	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	4	60	from	selenium	941:948	arg1	ASe-tps1					953:960	ASe-tps1	953:960	ASe-tps1	953:960	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	7	61	theme	structural	1584:1593	arg1	characteristics					1595:1609	different structural characteristics	1574:1609	different structural characteristics	1574:1609	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	0	62	from	characteristics	67:81	arg1	tea					145:147	synthetic selenized green tea	119:147	synthetic selenized green tea	119:147	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	0	62	from	characteristics	67:81	arg1	selenium-enriched					97:113	selenium-enriched	97:113	selenium-enriched	97:113	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	3	63	theme	acidic	598:603	arg1	heteropolysaccharides					605:625	acidic heteropolysaccharides	598:625	acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content	598:702	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	4	64	theme	ester	916:920	arg1	form					900:903	the form	896:903	the form of selenyl ester	896:920	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	3	65	theme	monosaccharide	642:655	arg1	composition					657:667	different monosaccharide composition	632:667	different monosaccharide composition	632:667	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
31734359	4	66	from	bond	1041:1044	arg1	branch					1053:1058	the branch	1049:1058	the branch of the polysaccharide	1049:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	7	67	theme	selenium	1691:1698	arg1	form					1683:1686	the form	1679:1686	the form of selenium in polysaccharides	1679:1717	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	2	68	theme	tea	495:497	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	68	theme	tea	495:497	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	5	69	theme	analysis	1205:1212	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	7	70	theme	artificial	1501:1510	arg1	methods					1525:1531	artificial selenization methods	1501:1531	artificial selenization methods	1501:1531	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	5	71	theme	helix	1266:1270	arg1	structure					1272:1280	a triple helix structure	1257:1280	a triple helix structure	1257:1280	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	0	72	theme	selenized	129:137	arg1	tea					145:147	synthetic selenized green tea	119:147	synthetic selenized green tea	119:147	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	6	73	theme	heat	1389:1392	arg1	ability					1402:1408	heat release ability	1389:1408	heat release ability	1389:1408	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	7	74	theme	significant	1657:1667	arg1	effect					1669:1674	a significant effect	1655:1674	a significant effect	1655:1674	These results indicated that the difference in artificial selenization methods not only made the polysaccharide exhibit different structural characteristics and the hypoglycemic activity, but also had a significant effect on the form of selenium in polysaccharides.					
31734359	2	75	theme	selenium	440:447	arg1	activity					381:388	activity	381:388	activity	381:388	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	75	theme	selenium	440:447	arg1	characterization					402:417	structural characterization	391:417	structural characterization	391:417	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	75	theme	selenium	440:447	arg1	forms					431:435	present forms	423:435	present forms	423:435	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	4	76	theme	2D	770:771	arg1	analysis					777:784	2D NMR analysis	770:784	2D NMR analysis	770:784	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	76	theme	2D	770:771	arg1	1D					766:767	1D	766:767	1D	766:767	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	0	77	theme	existence	24:32	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of existence	0:32	Comparative analysis of existence form for selenium and structural characteristics in artificial selenium-enriched and synthetic selenized green tea polysaccharides.					
31734359	1	78	theme	many	285:288	arg1	Se-PSs					290:295	many Se-PSs	285:295	many Se-PSs	285:295	In recent years, selenium-enriched polysaccharides (Se-PS) have been paid more and more attention, and the activity of many Se-PSs has been studied but little on their structure.					
31734359	5	79	from	studies	1104:1110	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	5	80	theme	test	1163:1166	arg1	characteristics					1130:1144	the structural characteristics	1115:1144	the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis	1115:1212	Besides, a series of studies on the structural characteristics of the Congo red test, I2-KI reaction, FESEM, DSC, and XRD analysis showed that the two artificial Se-TPSs had a triple helix structure and more branches.					
31734359	2	81	theme	artificial	466:475	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	81	theme	artificial	466:475	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	82	theme	structural	391:400	arg1	characterization					402:417	structural characterization	391:417	structural characterization	391:417	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	6	83	theme	crystal	1348:1354	arg1	morphology					1356:1365	crystal morphology	1348:1365	crystal morphology	1348:1365	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	4	84	from	position	1009:1016	arg1	form					1025:1028	the form	1021:1028	the form of the SeH bond on the branch of the polysaccharide	1021:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	84	from	position	1009:1016	arg1	group					984:988	the hydroxyl group	971:988	the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide	971:1080	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	4	85	theme	C-6	856:858	arg1	position					860:867	the C-6 position	852:867	the C-6 position in the polysaccharide	852:889	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	2	86	dep	activity	381:388	arg1	the					377:379	the	377:379	the	377:379	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	87	from	activity	381:388	arg1	Se-TPS					516:521	Se-TPS	516:521	Se-TPS	516:521	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	2	87	from	activity	381:388	arg1	polysaccharides					499:513	two different artificial selenium-enriched tea polysaccharides	452:513	two different artificial selenium-enriched tea polysaccharides (Se-TPS)	452:522	This study aimed to investigate the activity, structural characterization and present forms of selenium in two different artificial selenium-enriched tea polysaccharides (Se-TPS).					
31734359	6	88	theme	α-glucosidase	1419:1431	arg1	activity					1444:1451	the α-glucosidase inhibition activity	1415:1451	the α-glucosidase inhibition activity	1415:1451	However, there were significant differences in crystal morphology, apparent morphology, heat release ability, and the α-glucosidase inhibition activity.					
31734359	4	89	theme	hydroxyl	834:841	arg1	group					843:847	the hydroxyl group	830:847	the hydroxyl group at the C-6 position in the polysaccharide	830:889	Structural investigations including FT-IR, Raman spectra and 1D, 2D NMR analysis revealed that selenium in CSe-tps1 replaced the hydroxyl group at the C-6 position in the polysaccharide with the form of selenyl ester, while most of the selenium in ASe-tps1 replaced the hydroxyl group at the C-1 and C-6 position in the form of the SeH bond on the branch of the polysaccharide.					
31734359	3	90	theme	physicochemical	529:543	arg1	analysis					545:552	The physicochemical analysis	525:552	The physicochemical analysis	525:552	The physicochemical analysis showed that both CSe-tps1 and ASe-tps1 were acidic heteropolysaccharides with different monosaccharide composition, molar ratio, and selenium content.					
30572059	0	0	theme	hydrogel	91:98	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	0	0	theme	hydrogel	91:98	arg1	characterization					26:41	rheological characterization	14:41	rheological characterization	14:41	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	4	1	theme	rheological	543:553	arg1	behavior					555:562	rheological behavior	543:562	rheological behavior of the gellan-pullulan hydrogel	543:594	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	1	2	theme	diverse	161:167	arg1	sectors					180:186	diverse industrial sectors	161:186	diverse industrial sectors	161:186	Hydrogels have found applications in diverse industrial sectors and majority of them are chemically modified.					
30572059	0	3	theme	composite	81:89	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	1	4	theme	industrial	169:178	arg1	sectors					180:186	diverse industrial sectors	161:186	diverse industrial sectors	161:186	Hydrogels have found applications in diverse industrial sectors and majority of them are chemically modified.					
30572059	4	5	theme	Arrhenius	626:634	arg1	models					636:641	power law and Arrhenius models	612:641	power law and Arrhenius models	612:641	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	0	6	theme	gellan	103:108	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	5	7	theme	novel	717:721	arg1	hydrogel					723:730	this novel hydrogel	712:730	this novel hydrogel	712:730	Interestingly, this novel hydrogel was quick-setting and exhibited good crosslinking and maintained its elasticity at high temperature and frequency.					
30572059	3	8	theme	composite	386:394	arg1	hydrogel					396:403	a novel composite hydrogel	378:403	a novel composite hydrogel using gellan and pullulan	378:429	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	3	8	theme	composite	386:394	arg1	polysaccharides					477:491	two commercially available biologically safe polysaccharides	432:491	two commercially available biologically safe polysaccharides	432:491	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	6	9	theme	data	883:886	arg1	analysis					855:862	Overall analysis	847:862	Overall analysis of the rheological data	847:886	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	0	10	theme	pullulan	114:121	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	3	11	theme	chemical	506:513	arg1	modifications					515:527	any chemical modifications	502:527	any chemical modifications	502:527	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	5	12	theme	high	815:818	arg1	temperature					820:830	high temperature	815:830	high temperature	815:830	Interestingly, this novel hydrogel was quick-setting and exhibited good crosslinking and maintained its elasticity at high temperature and frequency.					
30572059	2	13	theme	non-toxic	284:292	arg1	hydrogels					294:302	stable biocompatible non-toxic hydrogels	263:302	stable biocompatible non-toxic hydrogels	263:302	However, recently demand for stable biocompatible non-toxic hydrogels has increased significantly.					
30572059	6	14	theme	gellan	964:969	arg1	hydrogel					971:978	gellan hydrogel	964:978	gellan hydrogel	964:978	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	3	15	theme	available	449:457	arg1	hydrogel					396:403	a novel composite hydrogel	378:403	a novel composite hydrogel using gellan and pullulan	378:429	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	3	15	theme	available	449:457	arg1	polysaccharides					477:491	two commercially available biologically safe polysaccharides	432:491	two commercially available biologically safe polysaccharides	432:491	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	4	16	theme	power	612:616	arg1	law					618:620	power law	612:620	power law	612:620	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	5	17	theme	good	764:767	arg1	crosslinking					769:780	good crosslinking	764:780	good crosslinking	764:780	Interestingly, this novel hydrogel was quick-setting and exhibited good crosslinking and maintained its elasticity at high temperature and frequency.					
30572059	6	18	theme	Overall	847:853	arg1	analysis					855:862	Overall analysis	847:862	Overall analysis of the rheological data	847:886	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	1	19	theme	them	204:207	arg1	majority					192:199	majority	192:199	majority of them	192:207	Hydrogels have found applications in diverse industrial sectors and majority of them are chemically modified.					
30572059	0	20	theme	rheological	14:24	arg1	characterization					26:41	rheological characterization	14:41	rheological characterization	14:41	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	4	21	theme	flow	682:685	arg1	behavior					687:694	flow behavior	682:694	flow behavior	682:694	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	4	22	theme	law	618:620	arg1	models					636:641	power law and Arrhenius models	612:641	power law and Arrhenius models	612:641	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	3	23	dep	available	449:457	arg1	safe					472:475	safe	472:475	safe	472:475	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	4	24	theme	hydrogel	587:594	arg1	behavior					555:562	rheological behavior	543:562	rheological behavior of the gellan-pullulan hydrogel	543:594	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
30572059	6	25	theme	potential	938:946	arg1	advantages					948:957	potential advantages	938:957	potential advantages over gellan hydrogel	938:978	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	0	26	theme	thermostable	54:65	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	3	27	theme	novel	380:384	arg1	hydrogel					396:403	a novel composite hydrogel	378:403	a novel composite hydrogel using gellan and pullulan	378:429	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	3	27	theme	novel	380:384	arg1	polysaccharides					477:491	two commercially available biologically safe polysaccharides	432:491	two commercially available biologically safe polysaccharides	432:491	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	0	28	theme	novel	48:52	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	3	29	theme	current	337:343	arg1	study					345:349	The current study	333:349	The current study	333:349	The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.					
30572059	7	30	theme	composite	1063:1071	arg1	hydrogel					1073:1080	a composite hydrogel	1061:1080	a composite hydrogel solely made of gellan and pullulan	1061:1115	This is the first report of a composite hydrogel solely made of gellan and pullulan.					
30572059	2	31	theme	biocompatible	270:282	arg1	hydrogels					294:302	stable biocompatible non-toxic hydrogels	263:302	stable biocompatible non-toxic hydrogels	263:302	However, recently demand for stable biocompatible non-toxic hydrogels has increased significantly.					
30572059	7	32	theme	hydrogel	1073:1080	arg1	This					1033:1036	This	1033:1036	This	1033:1036	This is the first report of a composite hydrogel solely made of gellan and pullulan.					
30572059	7	32	theme	hydrogel	1073:1080	arg1	report					1051:1056	the first report	1041:1056	the first report of a composite hydrogel solely made of gellan and pullulan	1041:1115	This is the first report of a composite hydrogel solely made of gellan and pullulan.					
30572059	6	33	theme	synthesized	907:917	arg1	hydrogel					919:926	the synthesized hydrogel	903:926	the synthesized hydrogel	903:926	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	2	34	theme	stable	263:268	arg1	hydrogels					294:302	stable biocompatible non-toxic hydrogels	263:302	stable biocompatible non-toxic hydrogels	263:302	However, recently demand for stable biocompatible non-toxic hydrogels has increased significantly.					
30572059	6	35	theme	rheological	871:881	arg1	data					883:886	the rheological data	867:886	the rheological data	867:886	Overall analysis of the rheological data suggested that the synthesized hydrogel can offer potential advantages over gellan hydrogel as it was found to be more thermostable and elastic.					
30572059	0	36	theme	setting	73:79	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	0	37	theme	quick	67:71	arg1	hydrogel					91:98	a novel thermostable quick setting composite hydrogel	46:98	a novel thermostable quick setting composite hydrogel of gellan and pullulan	46:121	Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.					
30572059	7	38	theme	first	1045:1049	arg1	This					1033:1036	This	1033:1036	This	1033:1036	This is the first report of a composite hydrogel solely made of gellan and pullulan.					
30572059	7	38	theme	first	1045:1049	arg1	report					1051:1056	the first report	1041:1056	the first report of a composite hydrogel solely made of gellan and pullulan	1041:1115	This is the first report of a composite hydrogel solely made of gellan and pullulan.					
30572059	4	39	theme	gellan-pullulan	571:585	arg1	hydrogel					587:594	the gellan-pullulan hydrogel	567:594	the gellan-pullulan hydrogel	567:594	Furthermore, rheological behavior of the gellan-pullulan hydrogel was studied and power law and Arrhenius models were fit to understand consistency and flow behavior.					
31712144	4	0	theme	antioxidant	703:713	arg1	activities					715:724	antioxidant activities	703:724	antioxidant activities	703:724	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	9	1	theme	consumption	1479:1489	arg1	method					1491:1496	a high-efficient and low-energy consumption method	1447:1496	a high-efficient and low-energy consumption method for CPP extraction	1447:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	1	theme	consumption	1479:1489	arg1	extraction					1432:1441	enzyme-ultrasonic assisted extraction	1405:1441	enzyme-ultrasonic assisted extraction	1405:1441	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	2	from	method	1491:1496	arg1	summary					1396:1402	summary	1396:1402	summary	1396:1402	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	1	3	theme	structural	247:256	arg1	characteristics					258:272	structural characteristics	247:272	structural characteristics	247:272	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	3	4	theme	extraction	536:545	arg1	conditions					547:556	the optimum extraction conditions	524:556	the optimum extraction conditions of enzyme-ultrasonic assisted extraction	524:597	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	3	4	theme	extraction	536:545	arg1	Meanwhile					513:521	Meanwhile	513:521	Meanwhile	513:521	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	1	5	theme	antioxidant	278:288	arg1	activities					290:299	antioxidant activities	278:299	antioxidant activities	278:299	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	5	6	theme	assisted	910:917	arg1	extraction					919:928	enzyme-ultrasonic assisted extraction	892:928	enzyme-ultrasonic assisted extraction	892:928	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	7	theme	enzyme-ultrasonic	892:908	arg1	extraction					919:928	enzyme-ultrasonic assisted extraction	892:928	enzyme-ultrasonic assisted extraction	892:928	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	8	contain	possessed	930:938	arg2	yield					963:967	the highest extraction yield	940:967	the highest extraction yield (10.39 ± 0.04%)	940:983	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	8	contain	possessed	930:938	arg1	extraction					919:928	enzyme-ultrasonic assisted extraction	892:928	enzyme-ultrasonic assisted extraction	892:928	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	8	contain	possessed	930:938	arg2	%					982:982	10.39 ± 0.04%	970:982	10.39 ± 0.04%	970:982	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	8	9	theme	highest	1314:1320	arg1	effect					1333:1338	the highest scavenging effect	1310:1338	the highest scavenging effect of superoxide radical and lipids inhibiting ability	1310:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	6	10	theme	other	1041:1045	arg1	polysaccharides					1047:1061	the other polysaccharides	1037:1061	the other polysaccharides	1037:1061	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	7	11	theme	CPPe	1158:1161	arg1	similar					1168:1174	similar	1168:1174	similar	1168:1174	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	11	theme	CPPe	1158:1161	arg1	composition					1122:1132	the monosaccharide composition	1103:1132	the monosaccharide composition of CPPc, CPPh, CPPu and CPPe	1103:1161	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	1	12	from	methods	133:139	arg1	CPP					194:196	CPP	194:196	CPP	194:196	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	1	12	from	methods	133:139	arg1	Bunge					187:191	Bunge	187:191	Bunge	187:191	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	3	13	theme	optimum	528:534	arg1	conditions					547:556	the optimum extraction conditions	524:556	the optimum extraction conditions of enzyme-ultrasonic assisted extraction	524:597	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	3	13	theme	optimum	528:534	arg1	Meanwhile					513:521	Meanwhile	513:521	Meanwhile	513:521	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	8	14	theme	inhibiting	1373:1382	arg1	ability					1384:1390	inhibiting ability	1373:1390	inhibiting ability	1373:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	3	15	theme	assisted	579:586	arg1	extraction					588:597	enzyme-ultrasonic assisted extraction	561:597	enzyme-ultrasonic assisted extraction	561:597	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	5	16	theme	highest	944:950	arg1	yield					963:967	the highest extraction yield	940:967	the highest extraction yield (10.39 ± 0.04%)	940:983	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	16	theme	highest	944:950	arg1	%					982:982	10.39 ± 0.04%	970:982	10.39 ± 0.04%	970:982	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	7	17	theme	monosaccharide	1205:1218	arg1	different					1225:1233	different	1225:1233	different	1225:1233	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	17	theme	monosaccharide	1205:1218	arg1	percentages					1190:1200	the molar percentages	1180:1200	the molar percentages of monosaccharide	1180:1218	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	18	theme	CPPu	1149:1152	arg1	similar					1168:1174	similar	1168:1174	similar	1168:1174	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	18	theme	CPPu	1149:1152	arg1	composition					1122:1132	the monosaccharide composition	1103:1132	the monosaccharide composition of CPPc, CPPh, CPPu and CPPe	1103:1161	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	3	19	theme	extraction	588:597	arg1	conditions					547:556	the optimum extraction conditions	524:556	the optimum extraction conditions of enzyme-ultrasonic assisted extraction	524:597	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	3	19	theme	extraction	588:597	arg1	Meanwhile					513:521	Meanwhile	513:521	Meanwhile	513:521	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	8	20	theme	scavenging	1322:1331	arg1	effect					1333:1338	the highest scavenging effect	1310:1338	the highest scavenging effect of superoxide radical and lipids inhibiting ability	1310:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	2	21	theme	assisted	387:394	arg1	CPPu					408:411	CPPu	408:411	CPPu	408:411	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	21	theme	assisted	387:394	arg1	extraction					396:405	ultrasound assisted extraction	376:405	ultrasound assisted extraction (CPPu)	376:412	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	0	22	theme	extraction	24:33	arg1	methods					35:41	different extraction methods	14:41	different extraction methods	14:41	Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge.					
31712144	4	23	theme	visual	760:765	arg1	photos					767:772	visual photos	760:772	visual photos	760:772	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	4	24	theme	gas	775:777	arg1	chromatography					779:792	gas chromatography	775:792	gas chromatography	775:792	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	2	25	theme	assisted	422:429	arg1	CPPe					443:446	CPPe	443:446	CPPe	443:446	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	25	theme	assisted	422:429	arg1	extraction					431:440	enzyme assisted extraction	415:440	enzyme assisted extraction (CPPe)	415:447	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	0	26	theme	different	14:22	arg1	methods					35:41	different extraction methods	14:41	different extraction methods	14:41	Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge.					
31712144	9	27	theme	assisted	1423:1430	arg1	method					1491:1496	a high-efficient and low-energy consumption method	1447:1496	a high-efficient and low-energy consumption method for CPP extraction	1447:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	27	theme	assisted	1423:1430	arg1	extraction					1432:1441	enzyme-ultrasonic assisted extraction	1405:1441	enzyme-ultrasonic assisted extraction	1405:1441	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	7	28	theme	CPPh	1143:1146	arg1	similar					1168:1174	similar	1168:1174	similar	1168:1174	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	28	theme	CPPh	1143:1146	arg1	composition					1122:1132	the monosaccharide composition	1103:1132	the monosaccharide composition of CPPc, CPPh, CPPu and CPPe	1103:1161	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	3	29	theme	enzyme-ultrasonic	561:577	arg1	extraction					588:597	enzyme-ultrasonic assisted extraction	561:597	enzyme-ultrasonic assisted extraction	561:597	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	2	30	theme	enzyme	415:420	arg1	CPPe					443:446	CPPe	443:446	CPPe	443:446	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	30	theme	enzyme	415:420	arg1	extraction					431:440	enzyme assisted extraction	415:440	enzyme assisted extraction (CPPe)	415:447	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	8	31	theme	activities	1272:1281	arg1	results					1249:1255	the results	1245:1255	the results of antioxidant activities	1245:1281	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	8	32	theme	radical	1354:1360	arg1	effect					1333:1338	the highest scavenging effect	1310:1338	the highest scavenging effect of superoxide radical and lipids inhibiting ability	1310:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	0	33	from	Bunge	90:94	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from Crataegus pinnatifida Bunge	47:94	Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge.					
31712144	4	34	theme	structural	672:681	arg1	characteristics					683:697	structural characteristics	672:697	structural characteristics	672:697	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	4	35	theme	extraction	653:662	arg1	yields					664:669	The extraction yields	649:669	The extraction yields	649:669	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	2	36	theme	assisted	471:478	arg1	CPPc					492:495	CPPc	492:495	CPPc	492:495	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	36	theme	assisted	471:478	arg1	extraction					480:489	enzyme-ultrasound assisted extraction	453:489	enzyme-ultrasound assisted extraction (CPPc)	453:496	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	0	37	theme	methods	35:41	arg1	Comparison					0:9	Comparison	0:9	Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge.	0:95	Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge.					
31712144	8	38	theme	superoxide	1343:1352	arg1	radical					1354:1360	superoxide radical	1343:1360	superoxide radical	1343:1360	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	3	39	theme	response	618:625	arg1	method					635:640	response surface method	618:640	response surface method (RSM)	618:646	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	3	39	theme	response	618:625	arg1	RSM					643:645	RSM	643:645	RSM	643:645	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	9	40	theme	CPP	1502:1504	arg1	extraction					1506:1515	CPP extraction	1502:1515	CPP extraction	1502:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	8	41	theme	lipids	1366:1371	arg1	effect					1333:1338	the highest scavenging effect	1310:1338	the highest scavenging effect of superoxide radical and lipids inhibiting ability	1310:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	2	42	theme	enzyme-ultrasound	453:469	arg1	CPPc					492:495	CPPc	492:495	CPPc	492:495	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	42	theme	enzyme-ultrasound	453:469	arg1	extraction					480:489	enzyme-ultrasound assisted extraction	453:489	enzyme-ultrasound assisted extraction (CPPc)	453:496	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	6	43	theme	CPPh	1010:1013	arg1	highest					1023:1029	highest	1023:1029	highest	1023:1029	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	6	43	theme	CPPh	1010:1013	arg1	weight					1000:1005	The molecular weight	986:1005	The molecular weight of CPPh	986:1013	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	7	44	theme	monosaccharide	1107:1120	arg1	similar					1168:1174	similar	1168:1174	similar	1168:1174	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	44	theme	monosaccharide	1107:1120	arg1	composition					1122:1132	the monosaccharide composition	1103:1132	the monosaccharide composition of CPPc, CPPh, CPPu and CPPe	1103:1161	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	9	45	theme	enzyme-ultrasonic	1405:1421	arg1	method					1491:1496	a high-efficient and low-energy consumption method	1447:1496	a high-efficient and low-energy consumption method for CPP extraction	1447:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	45	theme	enzyme-ultrasonic	1405:1421	arg1	extraction					1432:1441	enzyme-ultrasonic assisted extraction	1405:1441	enzyme-ultrasonic assisted extraction	1405:1441	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	1	46	theme	different	112:120	arg1	methods					133:139	different extraction methods	112:139	different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP)	112:197	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	7	47	theme	CPPc	1137:1140	arg1	similar					1168:1174	similar	1168:1174	similar	1168:1174	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	47	theme	CPPc	1137:1140	arg1	composition					1122:1132	the monosaccharide composition	1103:1132	the monosaccharide composition of CPPc, CPPh, CPPu and CPPe	1103:1161	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	9	48	theme	high-efficient	1449:1462	arg1	method					1491:1496	a high-efficient and low-energy consumption method	1447:1496	a high-efficient and low-energy consumption method for CPP extraction	1447:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	48	theme	high-efficient	1449:1462	arg1	extraction					1432:1441	enzyme-ultrasonic assisted extraction	1405:1441	enzyme-ultrasonic assisted extraction	1405:1441	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	8	49	theme	antioxidant	1260:1270	arg1	activities					1272:1281	antioxidant activities	1260:1281	antioxidant activities	1260:1281	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	1	50	theme	extraction	122:131	arg1	methods					133:139	different extraction methods	112:139	different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP)	112:197	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	1	51	theme	polysaccharides	144:158	arg1	methods					133:139	different extraction methods	112:139	different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP)	112:197	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	4	52	theme	infrared	837:844	arg1	spectroscopy					846:857	Fourier-transform infrared spectroscopy	819:857	Fourier-transform infrared spectroscopy	819:857	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	2	53	theme	water	351:355	arg1	extraction					357:366	hot water extraction	347:366	hot water extraction (CPPh)	347:373	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	53	theme	water	351:355	arg1	CPPh					369:372	CPPh	369:372	CPPh	369:372	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	5	54	theme	extraction	952:961	arg1	yield					963:967	the highest extraction yield	940:967	the highest extraction yield (10.39 ± 0.04%)	940:983	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	5	54	theme	extraction	952:961	arg1	%					982:982	10.39 ± 0.04%	970:982	10.39 ± 0.04%	970:982	The results clearly showed that enzyme-ultrasonic assisted extraction possessed the highest extraction yield (10.39 ± 0.04%).					
31712144	9	55	theme	low-energy	1468:1477	arg1	method					1491:1496	a high-efficient and low-energy consumption method	1447:1496	a high-efficient and low-energy consumption method for CPP extraction	1447:1515	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	9	55	theme	low-energy	1468:1477	arg1	extraction					1432:1441	enzyme-ultrasonic assisted extraction	1405:1441	enzyme-ultrasonic assisted extraction	1405:1441	In summary, enzyme-ultrasonic assisted extraction was a high-efficient and low-energy consumption method for CPP extraction.					
31712144	4	56	theme	Fourier-transform	819:835	arg1	spectroscopy					846:857	Fourier-transform infrared spectroscopy	819:857	Fourier-transform infrared spectroscopy	819:857	The extraction yields, structural characteristics and antioxidant activities were investigated and compared by visual photos, gas chromatography, ultraviolet-visible and Fourier-transform infrared spectroscopy.					
31712144	2	57	theme	hot	347:349	arg1	extraction					357:366	hot water extraction	347:366	hot water extraction (CPPh)	347:373	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	57	theme	hot	347:349	arg1	CPPh					369:372	CPPh	369:372	CPPh	369:372	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	1	58	theme	extraction	229:238	arg1	yield					240:244	the extraction yield	225:244	the extraction yield	225:244	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	6	59	contain	had	1063:1065	arg2	difference					1082:1091	no significant difference	1067:1091	no significant difference	1067:1091	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	6	59	contain	had	1063:1065	arg1	polysaccharides					1047:1061	the other polysaccharides	1037:1061	the other polysaccharides	1037:1061	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	3	60	theme	surface	627:633	arg1	method					635:640	response surface method	618:640	response surface method (RSM)	618:646	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	3	60	theme	surface	627:633	arg1	RSM					643:645	RSM	643:645	RSM	643:645	Meanwhile, the optimum extraction conditions of enzyme-ultrasonic assisted extraction were determined by response surface method (RSM).					
31712144	2	61	theme	ultrasound	376:385	arg1	CPPu					408:411	CPPu	408:411	CPPu	408:411	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	2	61	theme	ultrasound	376:385	arg1	extraction					396:405	ultrasound assisted extraction	376:405	ultrasound assisted extraction (CPPu)	376:412	Firstly, polysaccharides were obtained using hot water extraction (CPPh), ultrasound assisted extraction (CPPu), enzyme assisted extraction (CPPe) and enzyme-ultrasound assisted extraction (CPPc), respectively.					
31712144	7	62	theme	molar	1184:1188	arg1	different					1225:1233	different	1225:1233	different	1225:1233	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	7	62	theme	molar	1184:1188	arg1	percentages					1190:1200	the molar percentages	1180:1200	the molar percentages of monosaccharide	1180:1218	Besides, the monosaccharide composition of CPPc, CPPh, CPPu and CPPe were similar but the molar percentages of monosaccharide were different.					
31712144	6	63	theme	molecular	990:998	arg1	highest					1023:1029	highest	1023:1029	highest	1023:1029	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	6	63	theme	molecular	990:998	arg1	weight					1000:1005	The molecular weight	986:1005	The molecular weight of CPPh	986:1013	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
31712144	8	64	dep	radical	1354:1360	arg1	ability					1384:1390	inhibiting ability	1373:1390	inhibiting ability	1373:1390	Finally, the results of antioxidant activities showed that CPPc exhibited the highest scavenging effect of superoxide radical and lipids inhibiting ability.					
31712144	1	65	from	Bunge	187:191	arg1	methods					133:139	different extraction methods	112:139	different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP)	112:197	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	1	65	from	Bunge	187:191	arg1	polysaccharides					144:158	polysaccharides	144:158	polysaccharides from Crataegus pinnatifida Bunge (CPP)	144:197	In this study, different extraction methods of polysaccharides from Crataegus pinnatifida Bunge (CPP) were compared by studying the extraction yield, structural characteristics and antioxidant activities.					
31712144	6	66	theme	significant	1070:1080	arg1	difference					1082:1091	no significant difference	1067:1091	no significant difference	1067:1091	The molecular weight of CPPh was the highest while the other polysaccharides had no significant difference.					
30446149	6	0	contain	have	877:880	arg1	TPS					840:842	TPS	840:842	TPS from Trichoderma kanganensis	840:871	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	6	0	contain	have	877:880	arg2	application					892:902	potential application	882:902	potential application	882:902	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	3	1	theme	TPS	432:434	arg1	backbone					420:427	The major backbone	410:427	The major backbone of TPS	410:434	The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→.					
30446149	0	2	theme	Trichoderma	96:106	arg1	kanganensis					108:118	Trichoderma kanganensis	96:118	Trichoderma kanganensis	96:118	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	0	3	from	characteristics	11:25	arg1	kanganensis					108:118	Trichoderma kanganensis	96:118	Trichoderma kanganensis	96:118	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	6	4	theme	Trichoderma	849:859	arg1	kanganensis					861:871	Trichoderma kanganensis	849:871	Trichoderma kanganensis	849:871	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	3	5	theme	side	507:510	arg1	α-d-Glcp-1→4-α-d-Glcp-1→					523:546	α-d-Glcp-1→4-α-d-Glcp-1→	523:546	α-d-Glcp-1→4-α-d-Glcp-1→	523:546	The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→.					
30446149	3	5	theme	side	507:510	arg1	chains					512:517	the side chains	503:517	the side chains	503:517	The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→.					
30446149	4	6	theme	colon	700:704	arg1	cells					706:710	mouse colon cells	694:710	mouse colon cells (CT26 cells)	694:723	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	6	theme	colon	700:704	arg1	cells					718:722	CT26 cells	713:722	CT26 cells	713:722	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	1	7	attach	isolated	180:187	arg2	polysaccharide					143:156	A novel water-soluble polysaccharide	121:156	A novel water-soluble polysaccharide designated as TPS	121:174	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	1	7	attach	isolated	180:187	arg1	mycelia					211:217	the fermentation mycelia	194:217	the fermentation mycelia of Trichoderma kanganensis	194:244	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	2	8	theme	monosaccharide	314:327	arg1	composition					329:339	the monosaccharide composition	310:339	the monosaccharide composition	310:339	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	4	9	from	cells	641:645	arg1	non-toxic					621:629	non-toxic	621:629	non-toxic	621:629	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	5	10	theme	hydrogen	783:790	arg1	peroxide					792:799	hydrogen peroxide	783:799	hydrogen peroxide	783:799	TPS also showed free radical scavenging activity against hydrogen peroxide.					
30446149	1	11	theme	fermentation	198:209	arg1	mycelia					211:217	the fermentation mycelia	194:217	the fermentation mycelia of Trichoderma kanganensis	194:244	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	5	12	theme	radical	747:753	arg1	activity					766:773	free radical scavenging activity	742:773	free radical scavenging activity against hydrogen peroxide	742:799	TPS also showed free radical scavenging activity against hydrogen peroxide.					
30446149	5	13	theme	free	742:745	arg1	activity					766:773	free radical scavenging activity	742:773	free radical scavenging activity against hydrogen peroxide	742:799	TPS also showed free radical scavenging activity against hydrogen peroxide.					
30446149	0	14	from	activities	54:63	arg1	kanganensis					108:118	Trichoderma kanganensis	96:118	Trichoderma kanganensis	96:118	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	4	15	theme	mouse	694:698	arg1	cells					706:710	mouse colon cells	694:710	mouse colon cells (CT26 cells)	694:723	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	15	theme	mouse	694:698	arg1	cells					718:722	CT26 cells	713:722	CT26 cells	713:722	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	16	from	non-toxic	621:629	arg1	cells					652:656	LO2 cells	648:656	LO2 cells	648:656	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	16	from	non-toxic	621:629	arg1	cells					641:645	normal cells	634:645	normal cells (LO2 cells)	634:657	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	0	17	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	4	18	theme	CT26	713:716	arg1	cells					706:710	mouse colon cells	694:710	mouse colon cells (CT26 cells)	694:723	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	18	theme	CT26	713:716	arg1	cells					718:722	CT26 cells	713:722	CT26 cells	713:722	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	1	19	theme	Trichoderma	222:232	arg1	kanganensis					234:244	Trichoderma kanganensis	222:244	Trichoderma kanganensis	222:244	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	0	20	theme	anticancer/antioxidant	31:52	arg1	activities					54:63	anticancer/antioxidant activities	31:63	anticancer/antioxidant activities	31:63	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	1	21	theme	novel	123:127	arg1	polysaccharide					143:156	A novel water-soluble polysaccharide	121:156	A novel water-soluble polysaccharide designated as TPS	121:174	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	1	22	theme	kanganensis	234:244	arg1	mycelia					211:217	the fermentation mycelia	194:217	the fermentation mycelia of Trichoderma kanganensis	194:244	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	0	23	from	kanganensis	108:118	arg1	polysaccharide					76:89	a novel polysaccharide	68:89	a novel polysaccharide from Trichoderma kanganensis	68:118	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	0	23	from	kanganensis	108:118	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	0	23	from	kanganensis	108:118	arg1	activities					54:63	anticancer/antioxidant activities	31:63	anticancer/antioxidant activities	31:63	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	1	24	theme	water-soluble	129:141	arg1	polysaccharide					143:156	A novel water-soluble polysaccharide	121:156	A novel water-soluble polysaccharide designated as TPS	121:174	A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis.					
30446149	5	25	theme	scavenging	755:764	arg1	activity					766:773	free radical scavenging activity	742:773	free radical scavenging activity against hydrogen peroxide	742:799	TPS also showed free radical scavenging activity against hydrogen peroxide.					
30446149	4	26	theme	normal	634:639	arg1	cells					652:656	LO2 cells	648:656	LO2 cells	648:656	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	26	theme	normal	634:639	arg1	cells					641:645	normal cells	634:645	normal cells (LO2 cells)	634:657	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	6	27	theme	biomedical	907:916	arg1	fields					918:923	biomedical fields	907:923	biomedical fields	907:923	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	2	28	theme	molecular	272:280	arg1	mass					282:285	a weight-average molecular mass	255:285	a weight-average molecular mass of 3.074 × 105 Da	255:303	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	2	29	contain	had	251:253	arg2	mass					282:285	a weight-average molecular mass	255:285	a weight-average molecular mass of 3.074 × 105 Da	255:303	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	2	29	contain	had	251:253	arg1	TPS					247:249	TPS	247:249	TPS	247:249	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	0	30	theme	novel	70:74	arg1	polysaccharide					76:89	a novel polysaccharide	68:89	a novel polysaccharide from Trichoderma kanganensis	68:118	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	2	31	theme	weight-average	257:270	arg1	mass					282:285	a weight-average molecular mass	255:285	a weight-average molecular mass of 3.074 × 105 Da	255:303	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	6	32	theme	potential	882:890	arg1	application					892:902	potential application	882:902	potential application	882:902	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	4	33	theme	cells	706:710	arg1	proliferation					677:689	the proliferation	673:689	the proliferation of mouse colon cells (CT26 cells)	673:723	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	2	34	theme	Da	302:303	arg1	mass					282:285	a weight-average molecular mass	255:285	a weight-average molecular mass of 3.074 × 105 Da	255:303	TPS had a weight-average molecular mass of 3.074 × 105 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%).					
30446149	3	35	theme	major	414:418	arg1	backbone					420:427	The major backbone	410:427	The major backbone of TPS	410:434	The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→.					
30446149	6	36	from	kanganensis	861:871	arg1	TPS					840:842	TPS	840:842	TPS from Trichoderma kanganensis	840:871	Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.					
30446149	4	37	theme	LO2	648:650	arg1	cells					652:656	LO2 cells	648:656	LO2 cells	648:656	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	4	37	theme	LO2	648:650	arg1	cells					641:645	normal cells	634:645	normal cells (LO2 cells)	634:657	In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells).					
30446149	0	38	theme	polysaccharide	76:89	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
30446149	0	38	theme	polysaccharide	76:89	arg1	activities					54:63	anticancer/antioxidant activities	31:63	anticancer/antioxidant activities	31:63	Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma kanganensis.					
31034685	4	0	theme	MS/MS	901:905	arg1	scans					907:911	three MS/MS scans	895:911	three MS/MS scans	895:911	MS/MS data were collected in data-dependent mode and three MS/MS scans were acquired after the full MS scan.					
31034685	10	1	theme	acids	1685:1689	arg1	combinations					1640:1651	different combinations	1630:1651	different combinations of α2-3- and α2-6-linked sialic acids	1630:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	1	2	contain	has	181:183	arg1	PGC					176:178	PGC	176:178	PGC	176:178	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	2	contain	has	181:183	arg1	chromatography					160:173	RATIONALE Porous graphic carbon chromatography	128:173	RATIONALE Porous graphic carbon chromatography (PGC)	128:179	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	2	contain	has	181:183	arg2	mechanism					197:205	a different mechanism	185:205	a different mechanism in the retention of tryptic peptides	185:242	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	2	3	theme	1100	688:691	arg1	system					698:703	an Agilent 1100 HPLC system	677:703	an Agilent 1100 HPLC system	677:703	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	1	4	with	PGC	328:330	arg1	spectrometry					349:360	tandem mass spectrometry	337:360	tandem mass spectrometry	337:360	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	10	5	theme	α2-6-linked	1666:1676	arg1	acids					1685:1689	α2-3- and α2-6-linked sialic acids	1656:1689	α2-3- and α2-6-linked sialic acids	1656:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	1	6	theme	different	187:195	arg1	mechanism					197:205	a different mechanism	185:205	a different mechanism in the retention of tryptic peptides	185:242	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	8	7	theme	Glycan	1188:1193	arg1	composition					1195:1205	Glycan composition	1188:1205	Glycan composition	1188:1205	Glycan composition and peptide sequence were manually annotated.					
31034685	10	8	gly	glycopeptides	1611:1623	arg2	glycopeptides					1611:1623	isomeric glycopeptides	1602:1623	isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids	1602:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	7	9	theme	identified	1092:1101	arg1	compounds					1103:1111	identified compounds	1092:1111	identified compounds	1092:1111	Chromatograms and MS/MS spectra of identified compounds were extracted with Masslynx (Waters) and imported to Origin for analysis.					
31034685	2	10	theme	liquid	600:605	arg1	LC/MS/MS					648:655	LC/MS/MS	648:655	LC/MS/MS	648:655	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	10	theme	liquid	600:605	arg1	spectrometry					634:645	a capillary liquid chromatography/tandem mass spectrometry	588:645	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	3	11	theme	HyperCarb	770:778	arg1	column					784:789	a HyperCarb PGC column	768:789	a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles	768:839	Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles.					
31034685	3	11	theme	HyperCarb	770:778	arg1	i.d. × 100 mm					799:811	300 μm i.d. × 100 mm	792:811	300 μm i.d. × 100 mm	792:811	Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles.					
31034685	1	12	theme	phosphorylation	403:417	arg1	quantitation					387:398	the quantitation	383:398	the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation	383:483	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	11	13	theme	post-translational	1961:1978	arg1	PTMs					1995:1998	PTMs	1995:1998	PTMs	1995:1998	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	11	13	theme	post-translational	1961:1978	arg1	modifications					1980:1992	post-translational modifications	1961:1992	post-translational modifications (PTMs)	1961:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	1	14	theme	tryptic	227:233	arg1	peptides					235:242	tryptic peptides	227:242	tryptic peptides	227:242	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	2	15	theme	mass	629:632	arg1	LC/MS/MS					648:655	LC/MS/MS	648:655	LC/MS/MS	648:655	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	15	theme	mass	629:632	arg1	spectrometry					634:645	a capillary liquid chromatography/tandem mass spectrometry	588:645	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	1	16	theme	site-specific	457:469	arg1	glycosylation					471:483	site-specific glycosylation	457:483	site-specific glycosylation	457:483	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	17	theme	graphic	145:151	arg1	PGC					176:178	PGC	176:178	PGC	176:178	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	17	theme	graphic	145:151	arg1	chromatography					160:173	RATIONALE Porous graphic carbon chromatography	128:173	RATIONALE Porous graphic carbon chromatography (PGC)	128:179	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	4	18	theme	full	937:940	arg1	MS					942:943	the full MS	933:943	the full MS scan	933:948	MS/MS data were collected in data-dependent mode and three MS/MS scans were acquired after the full MS scan.					
31034685	1	19	theme	glycosylation	471:483	arg1	phosphorylation					403:417	phosphorylation stoichiometry and characterization	403:452	phosphorylation	403:417	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	19	theme	glycosylation	471:483	arg1	characterization					437:452	characterization	437:452	characterization	437:452	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	20	from	mechanism	197:205	arg1	retention					214:222	the retention	210:222	the retention of tryptic peptides	210:242	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	5	21	theme	RAW	951:953	arg1	data					955:958	RAW data	951:958	RAW data	951:958	RAW data were transformed to .					
31034685	0	22	theme	mass	64:67	arg1	spectrometry					69:80	tandem mass spectrometry	57:80	tandem mass spectrometry for post-translational modification analysis	57:125	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	1	23	theme	mass	344:347	arg1	spectrometry					349:360	tandem mass spectrometry	337:360	tandem mass spectrometry	337:360	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	10	24	with	glycopeptides	1611:1623	arg1	combinations					1640:1651	different combinations	1630:1651	different combinations of α2-3- and α2-6-linked sialic acids	1630:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	2	25	theme	bovine	541:546	arg1	fetuin					548:553	bovine fetuin	541:553	bovine fetuin	541:553	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	25	theme	bovine	541:546	arg1	standards					513:521	protein standards	505:521	protein standards (horse myoglobin, bovine fetuin and β-casein)	505:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	11	26	theme	CONCLUSIONS	1786:1796	arg1	alternative					1912:1922	an alternative	1909:1922	an alternative in the proteomic characterization of post-translational modifications (PTMs)	1909:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	11	26	theme	CONCLUSIONS	1786:1796	arg1	PGC					1798:1800	CONCLUSIONS PGC	1786:1800	CONCLUSIONS PGC	1786:1800	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	2	27	dep	standards	513:521	arg1	fetuin					548:553	bovine fetuin	541:553	bovine fetuin	541:553	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	27	dep	standards	513:521	arg1	standards					513:521	protein standards	505:521	protein standards (horse myoglobin, bovine fetuin and β-casein)	505:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	27	dep	standards	513:521	arg1	β-casein					559:566	β-casein	559:566	β-casein	559:566	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	27	dep	standards	513:521	arg1	myoglobin					530:538	horse myoglobin	524:538	horse myoglobin	524:538	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	0	28	theme	modification	105:116	arg1	analysis					118:125	post-translational modification analysis	86:125	post-translational modification analysis	86:125	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	9	29	theme	phosphorylation	1334:1348	arg1	sites					1350:1354	specific phosphorylation sites	1325:1354	specific phosphorylation sites from β-casein	1325:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	2	30	theme	HPLC	693:696	arg1	system					698:703	an Agilent 1100 HPLC system	677:703	an Agilent 1100 HPLC system	677:703	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	10	31	theme	exoglycosidase	1760:1773	arg1	digestion					1775:1783	exoglycosidase digestion	1760:1783	exoglycosidase digestion	1760:1783	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	4	32	dep	MS	942:943	arg1	scan					945:948	scan	945:948	scan	945:948	MS/MS data were collected in data-dependent mode and three MS/MS scans were acquired after the full MS scan.					
31034685	2	33	theme	protein	505:511	arg1	fetuin					548:553	bovine fetuin	541:553	bovine fetuin	541:553	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	33	theme	protein	505:511	arg1	β-casein					559:566	β-casein	559:566	β-casein	559:566	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	33	theme	protein	505:511	arg1	standards					513:521	protein standards	505:521	protein standards (horse myoglobin, bovine fetuin and β-casein)	505:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	33	theme	protein	505:511	arg1	myoglobin					530:538	horse myoglobin	524:538	horse myoglobin	524:538	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	9	34	dep	phosphopeptide	1401:1414	arg1	the					1397:1399	the	1397:1399	the	1397:1399	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	0	35	theme	Online	0:5	arg1	chromatography					29:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	4	36	theme	data-dependent	871:884	arg1	mode					886:889	data-dependent mode	871:889	data-dependent mode	871:889	MS/MS data were collected in data-dependent mode and three MS/MS scans were acquired after the full MS scan.					
31034685	2	37	theme	Synapt	710:715	arg1	Waters					729:734	Waters	729:734	Waters	729:734	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	37	theme	Synapt	710:715	arg1	HDMS					723:726	a Synapt G2-Si HDMS	708:726	a Synapt G2-Si HDMS (Waters)	708:735	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	11	38	gly	glycosylation	1876:1888	arg1	peptides					1842:1849	peptides	1842:1849	peptides with phosphorylation and glycosylation	1842:1888	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	0	39	theme	graphic	14:20	arg1	chromatography					29:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	1	40	theme	RATIONALE	128:136	arg1	PGC					176:178	PGC	176:178	PGC	176:178	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	40	theme	RATIONALE	128:136	arg1	chromatography					160:173	RATIONALE Porous graphic carbon chromatography	128:173	RATIONALE Porous graphic carbon chromatography (PGC)	128:179	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	10	41	theme	protein	1572:1578	arg1	sialoglycosylation					1580:1597	protein sialoglycosylation	1572:1597	protein sialoglycosylation	1572:1597	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	1	42	theme	coupling	319:326	arg1	PGC					328:330	coupling PGC	319:330	coupling PGC with tandem mass spectrometry	319:360	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	10	43	theme	different	1630:1638	arg1	combinations					1640:1651	different combinations	1630:1651	different combinations of α2-3- and α2-6-linked sialic acids	1630:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	9	44	theme	non-phosphorylated	1424:1441	arg1	counterpart					1443:1453	its non-phosphorylated counterpart	1420:1453	its non-phosphorylated counterpart	1420:1453	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	11	45	theme	peptides	1842:1849	arg1	separation					1828:1837	superior separation	1819:1837	superior separation of peptides with phosphorylation and glycosylation	1819:1888	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	10	46	link	α2-6-linked	1666:1676	arg1	acids					1685:1689	α2-3- and α2-6-linked sialic acids	1656:1689	α2-3- and α2-6-linked sialic acids	1656:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	11	47	theme	polar	2004:2008	arg1	groups					2010:2015	polar groups	2004:2015	polar groups	2004:2015	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	10	48	theme	sialic	1678:1683	arg1	acids					1685:1689	α2-3- and α2-6-linked sialic acids	1656:1689	α2-3- and α2-6-linked sialic acids	1656:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	7	49	theme	compounds	1103:1111	arg1	Chromatograms					1057:1069	Chromatograms	1057:1069	Chromatograms	1057:1069	Chromatograms and MS/MS spectra of identified compounds were extracted with Masslynx (Waters) and imported to Origin for analysis.					
31034685	7	49	theme	compounds	1103:1111	arg1	spectra					1081:1087	MS/MS spectra	1075:1087	MS/MS spectra	1075:1087	Chromatograms and MS/MS spectra of identified compounds were extracted with Masslynx (Waters) and imported to Origin for analysis.					
31034685	11	50	with	peptides	1842:1849	arg1	glycosylation					1876:1888	glycosylation	1876:1888	glycosylation	1876:1888	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	11	50	with	peptides	1842:1849	arg1	phosphorylation					1856:1870	phosphorylation	1856:1870	phosphorylation	1856:1870	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	9	51	theme	reversed-phase	1484:1497	arg1	chromatography					1499:1512	reversed-phase chromatography	1484:1512	reversed-phase chromatography	1484:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	2	52	theme	Agilent	680:686	arg1	system					698:703	an Agilent 1100 HPLC system	677:703	an Agilent 1100 HPLC system	677:703	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	9	53	from	β-casein	1361:1368	arg1	sites					1350:1354	specific phosphorylation sites	1325:1354	specific phosphorylation sites from β-casein	1325:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	9	53	from	β-casein	1361:1368	arg1	stoichiometry					1308:1320	the stoichiometry	1304:1320	the stoichiometry of specific phosphorylation sites from β-casein	1304:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	11	54	theme	proteomic	1931:1939	arg1	characterization					1941:1956	the proteomic characterization	1927:1956	the proteomic characterization of post-translational modifications (PTMs)	1927:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	7	55	theme	MS/MS	1075:1079	arg1	spectra					1081:1087	MS/MS spectra	1075:1087	MS/MS spectra	1075:1087	Chromatograms and MS/MS spectra of identified compounds were extracted with Masslynx (Waters) and imported to Origin for analysis.					
31034685	11	56	from	alternative	1912:1922	arg1	characterization					1941:1956	the proteomic characterization	1927:1956	the proteomic characterization of post-translational modifications (PTMs)	1927:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	2	57	theme	capillary	590:598	arg1	LC/MS/MS					648:655	LC/MS/MS	648:655	LC/MS/MS	648:655	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	57	theme	capillary	590:598	arg1	spectrometry					634:645	a capillary liquid chromatography/tandem mass spectrometry	588:645	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	10	58	theme	combination	1731:1741	arg1	ratios					1716:1721	the ratios	1712:1721	the ratios of each combination	1712:1741	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	3	59	theme	PGC	780:782	arg1	column					784:789	a HyperCarb PGC column	768:789	a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles	768:839	Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles.					
31034685	3	59	theme	PGC	780:782	arg1	i.d. × 100 mm					799:811	300 μm i.d. × 100 mm	792:811	300 μm i.d. × 100 mm	792:811	Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles.					
31034685	8	60	theme	peptide	1211:1217	arg1	sequence					1219:1226	peptide sequence	1211:1226	peptide sequence	1211:1226	Glycan composition and peptide sequence were manually annotated.					
31034685	11	61	theme	modifications	1980:1992	arg1	characterization					1941:1956	the proteomic characterization	1927:1956	the proteomic characterization of post-translational modifications (PTMs)	1927:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	1	62	theme	characterization	437:452	arg1	quantitation					387:398	the quantitation	383:398	the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation	383:483	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	63	theme	Porous	138:143	arg1	PGC					176:178	PGC	176:178	PGC	176:178	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	63	theme	Porous	138:143	arg1	chromatography					160:173	RATIONALE Porous graphic carbon chromatography	128:173	RATIONALE Porous graphic carbon chromatography (PGC)	128:179	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	9	64	from	stoichiometry	1308:1320	arg1	β-casein					1361:1368	β-casein	1361:1368	β-casein	1361:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	2	65	theme	spectrometry	634:645	arg1	system					658:663	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	9	66	theme	accurate	1279:1286	arg1	quantitation					1288:1299	accurate quantitation	1279:1299	accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography	1279:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	1	67	theme	carbon	153:158	arg1	PGC					176:178	PGC	176:178	PGC	176:178	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	1	67	theme	carbon	153:158	arg1	chromatography					160:173	RATIONALE Porous graphic carbon chromatography	128:173	RATIONALE Porous graphic carbon chromatography (PGC)	128:179	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	6	68	theme	Swissprot	1027:1035	arg1	database					1037:1044	the Swissprot database	1023:1044	the Swissprot database	1023:1044	mgf by PLGS (Waters) and searched against the Swissprot database by Mascot.					
31034685	2	69	theme	chromatography/tandem	607:627	arg1	LC/MS/MS					648:655	LC/MS/MS	648:655	LC/MS/MS	648:655	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	69	theme	chromatography/tandem	607:627	arg1	spectrometry					634:645	a capillary liquid chromatography/tandem mass spectrometry	588:645	a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system	588:663	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	1	70	theme	peptides	235:242	arg1	retention					214:222	the retention	210:222	the retention of tryptic peptides	210:242	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	3	71	theme	3 μm	826:829	arg1	particles					831:839	3 μm particles	826:839	3 μm particles	826:839	Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles.					
31034685	0	72	theme	post-translational	86:103	arg1	modification					105:116	post-translational modification	86:116	post-translational modification analysis	86:125	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	9	73	theme	stoichiometry	1308:1320	arg1	quantitation					1288:1299	accurate quantitation	1279:1299	accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography	1279:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	9	74	theme	specific	1325:1332	arg1	sites					1350:1354	specific phosphorylation sites	1325:1354	specific phosphorylation sites from β-casein	1325:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	9	75	theme	sites	1350:1354	arg1	stoichiometry					1308:1320	the stoichiometry	1304:1320	the stoichiometry of specific phosphorylation sites from β-casein	1304:1368	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	10	76	theme	comprehensive	1538:1550	arg1	characterization					1552:1567	comprehensive characterization	1538:1567	comprehensive characterization of protein sialoglycosylation	1538:1597	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	0	77	theme	porous	7:12	arg1	chromatography					29:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	2	78	theme	G2-Si	717:721	arg1	Waters					729:734	Waters	729:734	Waters	729:734	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	78	theme	G2-Si	717:721	arg1	HDMS					723:726	a Synapt G2-Si HDMS	708:726	a Synapt G2-Si HDMS (Waters)	708:735	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	0	79	theme	carbon	22:27	arg1	chromatography					29:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography	0:42	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	9	80	theme	efficient	1373:1381	arg1	separation					1383:1392	efficient separation	1373:1392	efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography	1373:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	2	81	theme	METHODS	486:492	arg1	Digests					494:500	METHODS Digests	486:500	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein)	486:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	10	82	theme	sialoglycosylation	1580:1597	arg1	characterization					1552:1567	comprehensive characterization	1538:1567	comprehensive characterization of protein sialoglycosylation	1538:1597	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	11	83	theme	superior	1819:1826	arg1	separation					1828:1837	superior separation	1819:1837	superior separation of peptides with phosphorylation and glycosylation	1819:1888	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	2	84	theme	horse	524:528	arg1	standards					513:521	protein standards	505:521	protein standards (horse myoglobin, bovine fetuin and β-casein)	505:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	2	84	theme	horse	524:528	arg1	myoglobin					530:538	horse myoglobin	524:538	horse myoglobin	524:538	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	4	85	theme	MS/MS	842:846	arg1	data					848:851	MS/MS data	842:851	MS/MS data	842:851	MS/MS data were collected in data-dependent mode and three MS/MS scans were acquired after the full MS scan.					
31034685	0	86	theme	tandem	57:62	arg1	spectrometry					69:80	tandem mass spectrometry	57:80	tandem mass spectrometry for post-translational modification analysis	57:125	Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.					
31034685	9	87	theme	phosphopeptide	1401:1414	arg1	separation					1383:1392	efficient separation	1373:1392	efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography	1373:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	11	88	used	used	1901:1904	arg2	PGC					1798:1800	CONCLUSIONS PGC	1786:1800	CONCLUSIONS PGC	1786:1800	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	11	88	used	used	1901:1904	arg2	alternative					1912:1922	an alternative	1909:1922	an alternative in the proteomic characterization of post-translational modifications (PTMs)	1909:1999	CONCLUSIONS PGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post-translational modifications (PTMs) by polar groups.					
31034685	2	89	theme	standards	513:521	arg1	Digests					494:500	METHODS Digests	486:500	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein)	486:567	METHODS Digests of protein standards (horse myoglobin, bovine fetuin and β-casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2-Si HDMS (Waters).					
31034685	1	90	theme	tandem	337:342	arg1	spectrometry					349:360	tandem mass spectrometry	337:360	tandem mass spectrometry	337:360	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	9	91	dep	RESULTS	1253:1259	arg1	PGC/MS/MS					1261:1269	PGC/MS/MS	1261:1269	PGC/MS/MS	1261:1269	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	10	92	theme	isomeric	1602:1609	arg1	glycopeptides					1611:1623	isomeric glycopeptides	1602:1623	isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids	1602:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	9	93	theme	counterpart	1443:1453	arg1	separation					1383:1392	efficient separation	1373:1392	efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography	1373:1512	RESULTS PGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β-casein by efficient separation of the phosphopeptide and its non-phosphorylated counterpart, which cannot be achieved by reversed-phase chromatography.					
31034685	1	94	dep	phosphorylation	403:417	arg1	stoichiometry					419:431	stoichiometry	419:431	stoichiometry	419:431	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31034685	10	95	theme	α2-3-	1656:1660	arg1	acids					1685:1689	α2-3- and α2-6-linked sialic acids	1656:1689	α2-3- and α2-6-linked sialic acids	1656:1689	PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2-3- and α2-6-linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.					
31034685	1	96	theme	reversed-phase	258:271	arg1	chromatography					273:286	reversed-phase chromatography	258:286	reversed-phase chromatography	258:286	RATIONALE Porous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed-phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.					
31775630	9	0	theme	Dendrobium	1670:1679	arg1	moniliforme					1681:1691	Dendrobium moniliforme	1670:1691	Dendrobium moniliforme under different tissues	1670:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	7	1	theme	genes	1251:1255	arg1	patterns					1235:1242	the tissue-specific expression patterns	1204:1242	the tissue-specific expression patterns of the genes involved in polysaccharide pathway	1204:1290	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	2	2	theme	polysaccharide	365:378	arg1	biosynthesis					380:391	polysaccharide biosynthesis	365:391	polysaccharide biosynthesis in D. moniliforme	365:409	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	11	3	theme	medical	2166:2172	arg1	herb					2174:2177	this medical herb	2161:2177	this medical herb	2161:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	2	4	theme	medicinal	304:312	arg1	ingredients					314:324	the main medicinal ingredients	295:324	the main medicinal ingredients	295:324	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	2	4	theme	medicinal	304:312	arg1	Polysaccharides					275:289	Polysaccharides	275:289	Polysaccharides	275:289	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	3	5	theme	comprehensive	568:580	arg1	transcriptome					582:594	a comprehensive transcriptome	566:594	a comprehensive transcriptome of D. moniliforme	566:612	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	9	6	dep	CONCLUSIONS	1608:1618	arg1	determined					1623:1632	determined	1623:1632	determined the content of polysaccharides from Dendrobium moniliforme under different tissues	1623:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	9	6	dep	CONCLUSIONS	1608:1618	arg1	obtained					1725:1732	obtained	1725:1732	obtained a large number of differential genes by transcriptome sequencing	1725:1797	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	11	7	theme	regulatory	2123:2132	arg1	mechanism					2134:2142	the regulatory mechanism	2119:2142	the regulatory mechanism which operate in this medical herb	2119:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	3	8	theme	moniliforme	602:612	arg1	transcriptome					582:594	a comprehensive transcriptome	566:594	a comprehensive transcriptome of D. moniliforme	566:612	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	9	9	theme	large	1736:1740	arg1	number					1742:1747	a large number	1734:1747	a large number of differential genes	1734:1769	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	10	10	theme	genes	1843:1847	arg1	pool					1825:1828	a pool	1823:1828	a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme	1823:1909	This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme.					
31775630	1	11	theme	Chinese	227:233	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine due to bioactive constituents	215:272	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	7	12	theme	molecular	1388:1396	arg1	level					1398:1402	the molecular level	1384:1402	the molecular level	1384:1402	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	1	13	theme	BACKGROUND	133:142	arg1	Linnaeus					168:175	Linnaeus	168:175	Linnaeus	168:175	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	1	13	theme	BACKGROUND	133:142	arg1	moniliforme					155:165	BACKGROUND Dendrobium moniliforme	133:165	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz	133:183	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	9	14	from	moniliforme	1681:1691	arg1	polysaccharides					1649:1663	polysaccharides	1649:1663	polysaccharides from Dendrobium moniliforme under different tissues	1649:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	9	14	from	moniliforme	1681:1691	arg1	content					1638:1644	the content	1634:1644	the content of polysaccharides from Dendrobium moniliforme under different tissues	1634:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	9	15	theme	genes	1765:1769	arg1	number					1742:1747	a large number	1734:1747	a large number of differential genes	1734:1769	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	8	16	theme	polysaccharide	1461:1474	arg1	pathway					1476:1482	the polysaccharide pathway	1457:1482	the polysaccharide pathway	1457:1482	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	9	17	theme	transcriptome	1774:1786	arg1	sequencing					1788:1797	transcriptome sequencing	1774:1797	transcriptome sequencing	1774:1797	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	11	18	theme	pathways	1992:1999	arg1	characterization					1956:1971	characterization	1956:1971	characterization	1956:1971	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	11	18	theme	pathways	1992:1999	arg1	analysis					1943:1950	the comprehensive analysis	1925:1950	the comprehensive analysis	1925:1950	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	5	19	theme	glycoside	936:944	arg1	hydrolases					946:955	780 glycoside hydrolases	932:955	780 glycoside hydrolases (GHs)	932:961	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	19	theme	glycoside	936:944	arg1	GHs					958:960	GHs	958:960	GHs	958:960	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	0	20	theme	moniliforme	85:95	arg1	profiling					36:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	0	20	theme	moniliforme	85:95	arg1	mining					64:69	putative gene mining	50:69	putative gene mining	50:69	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	7	21	theme	tissue-specific	1208:1222	arg1	patterns					1235:1242	the tissue-specific expression patterns	1204:1242	the tissue-specific expression patterns of the genes involved in polysaccharide pathway	1204:1290	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	4	22	dep	RESULTS	615:621	arg1	generated					705:713	generated	705:713	was generated by performing transcriptome sequencing	701:752	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	11	23	theme	composition	2075:2085	arg1	diversity					2053:2061	the diversity	2049:2061	the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb	2049:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	0	24	theme	different	114:122	arg1	tissues					124:130	different tissues	114:130	different tissues	114:130	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	5	25	theme	carbohydrate-binding	1000:1019	arg1	CBMs					1030:1033	CBMs	1030:1033	CBMs	1030:1033	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	25	theme	carbohydrate-binding	1000:1019	arg1	modules					1021:1027	75 carbohydrate-binding modules	997:1027	75 carbohydrate-binding modules (CBMs)	997:1034	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	9	26	from	content	1638:1644	arg1	moniliforme					1681:1691	Dendrobium moniliforme	1670:1691	Dendrobium moniliforme under different tissues	1670:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	7	27	theme	regulation	1363:1372	arg1	understanding					1325:1337	understanding	1325:1337	understanding of the biosynthesis and regulation of DMP at the molecular level	1325:1402	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	8	28	theme	different	1534:1542	arg1	tissues					1544:1550	different tissues	1534:1550	different tissues	1534:1550	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	0	29	theme	Dendrobium	74:83	arg1	moniliforme					85:95	Dendrobium moniliforme	74:95	Dendrobium moniliforme	74:95	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	0	30	theme	Polysaccharide	0:13	arg1	profiling					36:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	10	31	from	biosynthesis	1861:1872	arg1	moniliforme					1899:1909	D. moniliforme	1896:1909	D. moniliforme	1896:1909	This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme.					
31775630	7	32	theme	biosynthesis	1346:1357	arg1	understanding					1325:1337	understanding	1325:1337	understanding of the biosynthesis and regulation of DMP at the molecular level	1325:1402	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	3	33	theme	de	503:504	arg1	sequencing					525:534	de novo transcriptome sequencing	503:534	de novo transcriptome sequencing	503:534	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	0	34	theme	pathway	28:34	arg1	profiling					36:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	8	35	theme	key	1413:1415	arg1	Susy					1431:1434	Susy	1431:1434	Susy	1431:1434	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	35	theme	key	1413:1415	arg1	genes					1424:1428	The two key enzyme genes	1405:1428	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway	1405:1482	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	35	theme	key	1413:1415	arg1	SPS					1440:1442	SPS	1440:1442	SPS	1440:1442	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	lyases					1059:1064	44 polysaccharide lyases	1041:1064	44 polysaccharide lyases (PLs)	1041:1070	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	unigenes					853:860	1204 carbohydrate-active related unigenes	820:860	1204 carbohydrate-active related unigenes	820:860	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	genes					919:923	417 glycosyltransferase genes	895:923	417 glycosyltransferase genes (GTs)	895:929	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	hydrolases					946:955	780 glycoside hydrolases	932:955	780 glycoside hydrolases (GHs)	932:961	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	esterases					980:988	19 carbohydrate esterases	964:988	19 carbohydrate esterases (CEs)	964:994	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	36	theme	carbohydrate-active	825:843	arg1	modules					1021:1027	75 carbohydrate-binding modules	997:1027	75 carbohydrate-binding modules (CBMs)	997:1034	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	7	37	dep	biosynthesis	1346:1357	arg1	the					1342:1344	the	1342:1344	the	1342:1344	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	8	38	theme	quantitative	1580:1591	arg1	PCR					1603:1605	quantitative real-time PCR	1580:1605	quantitative real-time PCR	1580:1605	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	39	from	patterns	1522:1529	arg1	tissues					1544:1550	different tissues	1534:1550	different tissues	1534:1550	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	5	40	theme	polysaccharide	1044:1057	arg1	PLs					1067:1069	PLs	1067:1069	PLs	1067:1069	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	40	theme	polysaccharide	1044:1057	arg1	lyases					1059:1064	44 polysaccharide lyases	1041:1064	44 polysaccharide lyases (PLs)	1041:1070	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	7	41	from	understanding	1325:1337	arg1	level					1398:1402	the molecular level	1384:1402	the molecular level	1384:1402	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	3	42	theme	transcriptome	511:523	arg1	sequencing					525:534	de novo transcriptome sequencing	503:534	de novo transcriptome sequencing	503:534	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	6	43	theme	differential	1110:1121	arg1	DEGs					1141:1144	DEGs	1141:1144	DEGs	1141:1144	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	6	43	theme	differential	1110:1121	arg1	genes					1134:1138	21 differential expression genes	1107:1138	21 differential expression genes (DEGs) related to polysaccharide	1107:1171	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	2	44	theme	D.	396:397	arg1	moniliforme					399:409	D. moniliforme	396:409	D. moniliforme	396:409	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	7	45	theme	polysaccharide	1269:1282	arg1	pathway					1284:1290	polysaccharide pathway	1269:1290	polysaccharide pathway	1269:1290	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	3	46	theme	first	544:548	arg1	time					550:553	the first time	540:553	the first time	540:553	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	6	47	theme	synthase	1090:1097	arg1	family					1099:1104	the cellulose synthase family	1076:1104	the cellulose synthase family	1076:1104	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	11	48	theme	chemical	2066:2073	arg1	composition					2075:2085	chemical composition	2066:2085	chemical composition	2066:2085	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	9	49	theme	different	1699:1707	arg1	tissues					1709:1715	different tissues	1699:1715	different tissues	1699:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	2	50	theme	main	299:302	arg1	ingredients					314:324	the main medicinal ingredients	295:324	the main medicinal ingredients	295:324	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	2	50	theme	main	299:302	arg1	Polysaccharides					275:289	Polysaccharides	275:289	Polysaccharides	275:289	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	0	51	theme	gene	59:62	arg1	mining					64:69	putative gene mining	50:69	putative gene mining	50:69	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	2	52	from	biosynthesis	380:391	arg1	moniliforme					399:409	D. moniliforme	396:409	D. moniliforme	396:409	Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme.					
31775630	11	53	theme	characteristics	2098:2112	arg1	diversity					2053:2061	the diversity	2049:2061	the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb	2049:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	1	54	theme	well-known	190:199	arg1	plant					201:205	a well-known plant	188:205	a well-known plant used in traditional Chinese medicine due to bioactive constituents	188:272	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	1	54	theme	well-known	190:199	arg1	Swartz					178:183	Swartz	178:183	Swartz	178:183	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	6	55	theme	related	1147:1153	arg1	DEGs					1141:1144	DEGs	1141:1144	DEGs	1141:1144	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	6	55	theme	related	1147:1153	arg1	genes					1134:1138	21 differential expression genes	1107:1138	21 differential expression genes (DEGs) related to polysaccharide	1107:1171	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	3	56	theme	D.	599:600	arg1	moniliforme					602:612	D. moniliforme	599:612	D. moniliforme	599:612	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	11	57	theme	mechanism	2134:2142	arg1	diversity					2053:2061	the diversity	2049:2061	the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb	2049:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	4	58	theme	unigenes	659:666	arg1	database					639:646	a database	637:646	a database of 562,580 unigenes (average length = 1115.67 bases)	637:699	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	5	59	theme	glycosyltransferase	899:917	arg1	GTs					926:928	GTs	926:928	GTs	926:928	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	59	theme	glycosyltransferase	899:917	arg1	genes					919:923	417 glycosyltransferase genes	895:923	417 glycosyltransferase genes (GTs)	895:929	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	4	60	theme	average	669:675	arg1	unigenes					659:666	562,580 unigenes	651:666	562,580 unigenes (average length = 1115.67 bases)	651:699	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	4	60	theme	average	669:675	arg1	bases					694:698	average length = 1115.67 bases	669:698	average length = 1115.67 bases	669:698	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	10	61	theme	candidate	1833:1841	arg1	genes					1843:1847	candidate genes	1833:1847	candidate genes involved in biosynthesis of polysaccharides in D. moniliforme	1833:1909	This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme.					
31775630	9	62	theme	differential	1752:1763	arg1	genes					1765:1769	differential genes	1752:1769	differential genes	1752:1769	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	10	63	theme	polysaccharides	1877:1891	arg1	biosynthesis					1861:1872	biosynthesis	1861:1872	biosynthesis of polysaccharides in D. moniliforme	1861:1909	This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme.					
31775630	1	64	theme	Dendrobium	144:153	arg1	Linnaeus					168:175	Linnaeus	168:175	Linnaeus	168:175	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	1	64	theme	Dendrobium	144:153	arg1	moniliforme					155:165	BACKGROUND Dendrobium moniliforme	133:165	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz	133:183	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	5	65	theme	transcriptome	791:803	arg1	annotation					773:782	the gene annotation	764:782	the gene annotation of the transcriptome	764:803	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	11	66	theme	better	2024:2029	arg1	insight					2031:2037	a better insight	2022:2037	a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb	2022:2177	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	8	67	dep	genes	1424:1428	arg1	Susy					1431:1434	Susy	1431:1434	Susy	1431:1434	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	67	dep	genes	1424:1428	arg1	genes					1424:1428	The two key enzyme genes	1405:1428	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway	1405:1482	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	67	dep	genes	1424:1428	arg1	SPS					1440:1442	SPS	1440:1442	SPS	1440:1442	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	7	68	theme	DMP	1377:1379	arg1	biosynthesis					1346:1357	biosynthesis	1346:1357	biosynthesis	1346:1357	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	7	68	theme	DMP	1377:1379	arg1	regulation					1363:1372	regulation	1363:1372	regulation	1363:1372	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	7	69	theme	expression	1224:1233	arg1	patterns					1235:1242	the tissue-specific expression patterns	1204:1242	the tissue-specific expression patterns of the genes involved in polysaccharide pathway	1204:1290	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	8	70	theme	expression	1511:1520	arg1	patterns					1522:1529	their expression patterns	1505:1529	their expression patterns in different tissues	1505:1550	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	4	71	theme	transcriptome	729:741	arg1	sequencing					743:752	transcriptome sequencing	729:752	transcriptome sequencing	729:752	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	5	72	theme	gene	768:771	arg1	annotation					773:782	the gene annotation	764:782	the gene annotation of the transcriptome	764:803	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	11	73	theme	synthetic	2088:2096	arg1	characteristics					2098:2112	synthetic characteristics	2088:2112	synthetic characteristics	2088:2112	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	6	74	theme	cellulose	1080:1088	arg1	family					1099:1104	the cellulose synthase family	1076:1104	the cellulose synthase family	1076:1104	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	0	75	theme	biosynthetic	15:26	arg1	profiling					36:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling	0:44	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	5	76	theme	carbohydrate	967:978	arg1	CEs					991:993	CEs	991:993	CEs	991:993	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	76	theme	carbohydrate	967:978	arg1	esterases					980:988	19 carbohydrate esterases	964:988	19 carbohydrate esterases (CEs)	964:994	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	3	77	theme	transcription	469:481	arg1	level					483:487	the transcription level	465:487	the transcription level	465:487	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	5	78	theme	CAZy	870:873	arg1	database					875:882	CAZy database	870:882	CAZy database	870:882	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	7	79	from	level	1398:1402	arg1	understanding					1325:1337	understanding	1325:1337	understanding of the biosynthesis and regulation of DMP at the molecular level	1325:1402	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	7	79	from	level	1398:1402	arg1	biosynthesis					1346:1357	biosynthesis	1346:1357	biosynthesis	1346:1357	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	7	79	from	level	1398:1402	arg1	regulation					1363:1372	regulation	1363:1372	regulation	1363:1372	Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level.					
31775630	0	80	theme	putative	50:57	arg1	mining					64:69	putative gene mining	50:69	putative gene mining	50:69	Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.					
31775630	1	81	theme	traditional	215:225	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine due to bioactive constituents	215:272	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	3	82	dep	de	503:504	arg1	novo					506:509	novo	506:509	novo	506:509	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	8	83	theme	real-time	1593:1601	arg1	PCR					1603:1605	quantitative real-time PCR	1580:1605	quantitative real-time PCR	1580:1605	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	10	84	theme	D.	1896:1897	arg1	moniliforme					1899:1909	D. moniliforme	1896:1909	D. moniliforme	1896:1909	This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme.					
31775630	11	85	theme	significant	1980:1990	arg1	pathways					1992:1999	the significant pathways	1976:1999	the significant pathways	1976:1999	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	5	86	theme	related	845:851	arg1	lyases					1059:1064	44 polysaccharide lyases	1041:1064	44 polysaccharide lyases (PLs)	1041:1070	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	86	theme	related	845:851	arg1	unigenes					853:860	1204 carbohydrate-active related unigenes	820:860	1204 carbohydrate-active related unigenes	820:860	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	86	theme	related	845:851	arg1	genes					919:923	417 glycosyltransferase genes	895:923	417 glycosyltransferase genes (GTs)	895:929	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	86	theme	related	845:851	arg1	hydrolases					946:955	780 glycoside hydrolases	932:955	780 glycoside hydrolases (GHs)	932:961	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	86	theme	related	845:851	arg1	esterases					980:988	19 carbohydrate esterases	964:988	19 carbohydrate esterases (CEs)	964:994	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	5	86	theme	related	845:851	arg1	modules					1021:1027	75 carbohydrate-binding modules	997:1027	75 carbohydrate-binding modules (CBMs)	997:1034	Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs).					
31775630	8	87	theme	enzyme	1417:1422	arg1	Susy					1431:1434	Susy	1431:1434	Susy	1431:1434	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	87	theme	enzyme	1417:1422	arg1	genes					1424:1428	The two key enzyme genes	1405:1428	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway	1405:1482	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	8	87	theme	enzyme	1417:1422	arg1	SPS					1440:1442	SPS	1440:1442	SPS	1440:1442	The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.					
31775630	6	88	theme	expression	1123:1132	arg1	DEGs					1141:1144	DEGs	1141:1144	DEGs	1141:1144	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	6	88	theme	expression	1123:1132	arg1	genes					1134:1138	21 differential expression genes	1107:1138	21 differential expression genes (DEGs) related to polysaccharide	1107:1171	In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified.					
31775630	1	89	theme	due	244:246	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine due to bioactive constituents	215:272	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
31775630	9	90	theme	polysaccharides	1649:1663	arg1	content					1638:1644	the content	1634:1644	the content of polysaccharides from Dendrobium moniliforme under different tissues	1634:1715	CONCLUSIONS We determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing.					
31775630	4	91	theme	562,580	651:657	arg1	unigenes					659:666	562,580 unigenes	651:666	562,580 unigenes (average length = 1115.67 bases)	651:699	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	4	91	theme	562,580	651:657	arg1	bases					694:698	average length = 1115.67 bases	669:698	average length = 1115.67 bases	669:698	RESULTS In our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing.					
31775630	3	92	from	level	483:487	arg1	polysaccharide					447:460	the polysaccharide	443:460	the polysaccharide at the transcription level	443:487	To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.					
31775630	11	93	theme	comprehensive	1929:1941	arg1	analysis					1943:1950	the comprehensive analysis	1925:1950	the comprehensive analysis	1925:1950	Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.					
31775630	1	94	theme	bioactive	251:259	arg1	constituents					261:272	bioactive constituents	251:272	bioactive constituents	251:272	BACKGROUND Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents.					
29620509	7	0	dep	lines	1026:1030	arg1	Result					989:994	Result	989:994	Result	989:994	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	5	1	dep	H.	761:762	arg1	removed					788:794	removed	788:794	was removed by using human rhinovirus protease and size exclusion chromatography	784:863	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	7	2	theme	protein	1063:1069	arg1	production					1042:1051	high production	1037:1051	high production of Necl-4 protein	1037:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	9	3	theme	AP	1359:1360	arg1	system					1391:1396	modified AP mammalian protein expression system	1350:1396	modified AP mammalian protein expression system	1350:1396	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	4	4	theme	inhibitor	609:617	arg1	kifunensine					619:629	N-glycosylation processing inhibitor kifunensine	582:629	N-glycosylation processing inhibitor kifunensine	582:629	Then, by adding N-glycosylation processing inhibitor kifunensine into the medium, complex glycan was inhibited to generate.					
29620509	9	5	theme	protein	1372:1378	arg1	system					1391:1396	modified AP mammalian protein expression system	1350:1396	modified AP mammalian protein expression system	1350:1396	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	8	6	theme	Necl-4	1190:1195	arg1	protein					1197:1203	purified Necl-4 protein	1181:1203	purified Necl-4 protein	1181:1203	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	7	7	theme	high	1037:1040	arg1	production					1042:1051	high production	1037:1051	high production of Necl-4 protein	1037:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	3	8	theme	AP	521:522	arg1	assay					533:537	the AP activity assay	517:537	the AP activity assay	517:537	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	1	9	theme	Nectin-like	176:186	arg1	protein					199:205	Nectin-like 4 (Necl-4) protein	176:205	Nectin-like 4 (Necl-4) protein	176:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	10	10	theme	inhibitor	1508:1516	arg1	kifunensine					1518:1528	mannosidase inhibitor kifunensine	1496:1528	mannosidase inhibitor kifunensine into the medium and cutting purified protein	1496:1573	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	2	11	theme	Necls	275:279	arg1	domain					265:270	the extracellular domain	247:270	the extracellular domain of Necls	247:279	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	2	11	theme	Necls	275:279	arg1	Necls					275:279	Necls	275:279	Necls	275:279	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	8	12	theme	soluble	1154:1160	arg1	form					1173:1176	The soluble and active form	1150:1176	The soluble and active form of purified Necl-4 protein	1150:1203	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	9	13	dep	Conclusions	1329:1339	arg1	screen					1413:1418	screen	1413:1418	can easily screen the high productive stable cell lines by using AP activity assay	1402:1483	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	4	14	theme	N-glycosylation	582:596	arg1	kifunensine					619:629	N-glycosylation processing inhibitor kifunensine	582:629	N-glycosylation processing inhibitor kifunensine	582:629	Then, by adding N-glycosylation processing inhibitor kifunensine into the medium, complex glycan was inhibited to generate.					
29620509	10	15	theme	cutting	1550:1556	arg1	protein					1567:1573	cutting purified protein	1550:1573	cutting purified protein	1550:1573	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	11	16	theme	structural	1758:1767	arg1	studies					1784:1790	structural and functional studies	1758:1790	structural and functional studies	1758:1790	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	7	17	theme	stable	1014:1019	arg1	lines					1026:1030	The transient and stable cell lines	996:1030	Result The transient and stable cell lines with high production of Necl-4 protein	989:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	3	18	theme	Western	543:549	arg1	analysis					556:563	Western blot analysis	543:563	Western blot analysis	543:563	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	8	19	theme	active	1166:1171	arg1	form					1173:1176	The soluble and active form	1150:1176	The soluble and active form of purified Necl-4 protein	1150:1203	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	6	20	theme	protein	903:909	arg1	concentration					870:882	The concentration	866:882	The concentration of purified Necl-4 protein	866:909	The concentration of purified Necl-4 protein was monitored by measuring the absorbance at 280 nm and analyzed by SDS-PAGE.					
29620509	11	21	theme	glycoprotein	1741:1752	arg1	purification					1725:1736	purification	1725:1736	purification	1725:1736	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	11	21	theme	glycoprotein	1741:1752	arg1	expression					1710:1719	expression	1710:1719	expression	1710:1719	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	7	22	theme	transient	1000:1008	arg1	lines					1026:1030	The transient and stable cell lines	996:1030	Result The transient and stable cell lines with high production of Necl-4 protein	989:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	6	23	theme	purified	887:894	arg1	protein					903:909	purified Necl-4 protein	887:909	purified Necl-4 protein	887:909	The concentration of purified Necl-4 protein was monitored by measuring the absorbance at 280 nm and analyzed by SDS-PAGE.					
29620509	8	24	theme	N-glycan	1250:1257	arg1	protein					1259:1265	native N-glycan protein	1243:1265	native N-glycan protein	1243:1265	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	9	25	theme	productive	1429:1438	arg1	lines					1452:1456	the high productive stable cell lines	1420:1456	the high productive stable cell lines	1420:1456	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	1	26	theme	transient	120:128	arg1	lines					146:150	the transient and stable cell lines	116:150	the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein	116:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	0	27	theme	Glycoprotein	63:74	arg1	Purification					27:38	Purification	27:38	Purification	27:38	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	0	27	theme	Glycoprotein	63:74	arg1	Expression					12:21	Expression	12:21	Expression	12:21	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	8	28	gly	deglycosylation	1224:1238	arg1	protein					1259:1265	native N-glycan protein	1243:1265	native N-glycan protein	1243:1265	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	5	29	theme	size	835:838	arg1	chromatography					850:863	human rhinovirus protease and size exclusion chromatography	805:863	chromatography	850:863	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	11	30	theme	functional	1773:1782	arg1	studies					1784:1790	structural and functional studies	1758:1790	structural and functional studies	1758:1790	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	4	31	theme	complex	648:654	arg1	glycan					656:661	complex glycan	648:661	complex glycan	648:661	Then, by adding N-glycosylation processing inhibitor kifunensine into the medium, complex glycan was inhibited to generate.					
29620509	0	32	theme	293ET	79:83	arg1	Line					90:93	293ET Cell Line	79:93	293ET Cell Line	79:93	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	7	33	from	reaction	1098:1105	arg1	vector					1142:1147	the recombinant vector	1126:1147	the recombinant vector	1126:1147	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	9	34	theme	AP	1467:1468	arg1	assay					1479:1483	AP activity assay	1467:1483	AP activity assay	1467:1483	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	8	35	theme	native	1243:1248	arg1	protein					1259:1265	native N-glycan protein	1243:1265	native N-glycan protein	1243:1265	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	8	36	theme	mg/L	1297:1300	arg1	culture					1302:1308	4 mg/L culture	1295:1308	4 mg/L culture	1295:1308	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	10	37	theme	endoglycosidase	1584:1598	arg1	H					1600:1600	endoglycosidase H	1584:1600	endoglycosidase H	1584:1600	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	5	38	theme	residual	694:701	arg1	glycan					703:708	The residual glycan	690:708	The residual glycan of purified protein	690:728	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	0	39	theme	Mouse	43:47	arg1	Glycoprotein					63:74	Mouse Nectin-like 4 Glycoprotein	43:74	Mouse Nectin-like 4 Glycoprotein	43:74	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	6	40	from	nm	960:961	arg1	absorbance					942:951	the absorbance	938:951	the absorbance at 280 nm	938:961	The concentration of purified Necl-4 protein was monitored by measuring the absorbance at 280 nm and analyzed by SDS-PAGE.					
29620509	2	41	theme	alkaline	346:353	arg1	phosphatase					355:365	alkaline phosphatase	346:365	alkaline phosphatase (AP)	346:370	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	2	41	theme	alkaline	346:353	arg1	AP					368:369	AP	368:369	AP	368:369	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	0	42	theme	Recombinant	0:10	arg1	Expression					12:21	Expression	12:21	Expression	12:21	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	2	43	theme	cDNA	223:226	arg1	sequences					228:236	cDNA sequences	223:236	cDNA sequences encoding the extracellular domain of Necls	223:279	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	2	44	theme	fusion	376:381	arg1	expression					383:392	fusion expression	376:392	fusion expression	376:392	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	5	45	theme	AP	773:774	arg1	protein					776:782	AP protein	773:782	AP protein	773:782	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	3	46	theme	culture	479:485	arg1	medium					487:492	the culture medium	475:492	the culture medium which were detected by the AP activity assay and Western blot analysis	475:563	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	9	47	theme	stable	1440:1445	arg1	lines					1452:1456	the high productive stable cell lines	1420:1456	the high productive stable cell lines	1420:1456	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	8	48	theme	%	1326:1326	arg1	purity					1314:1319	purity	1314:1319	purity	1314:1319	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	8	48	theme	%	1326:1326	arg1	culture					1302:1308	4 mg/L culture	1295:1308	4 mg/L culture	1295:1308	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	3	49	theme	293ET	401:405	arg1	cells					407:411	293ET cells	401:411	293ET cells	401:411	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	9	50	theme	mammalian	1362:1370	arg1	system					1391:1396	modified AP mammalian protein expression system	1350:1396	modified AP mammalian protein expression system	1350:1396	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	7	51	with	lines	1026:1030	arg1	production					1042:1051	high production	1037:1051	high production of Necl-4 protein	1037:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	8	52	with	deglycosylation	1224:1238	arg1	level					1286:1290	an expression level	1272:1290	an expression level of 4 mg/L culture and purity of 95%	1272:1326	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	0	53	theme	Nectin-like	49:59	arg1	Glycoprotein					63:74	Mouse Nectin-like 4 Glycoprotein	43:74	Mouse Nectin-like 4 Glycoprotein	43:74	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	11	54	dep	expression	1710:1719	arg1	the					1706:1708	the	1706:1708	the	1706:1708	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	3	55	theme	Necls-AP	430:437	arg1	protein					446:452	Necls-AP fusion protein	430:452	Necls-AP fusion protein	430:452	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	1	56	theme	protein	199:205	arg1	production					162:171	high production	157:171	high production of Nectin-like 4 (Necl-4) protein	157:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	9	57	theme	cell	1447:1450	arg1	lines					1452:1456	the high productive stable cell lines	1420:1456	the high productive stable cell lines	1420:1456	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	5	58	theme	human	805:809	arg1	protease					822:829	human rhinovirus protease and size exclusion chromatography	805:863	protease	822:829	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	5	59	theme	purified	713:720	arg1	protein					722:728	purified protein	713:728	purified protein	713:728	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	10	60	theme	milligram	1650:1658	arg1	quantities					1660:1669	milligram quantities	1650:1669	milligram quantities	1650:1669	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	10	61	theme	mannosidase	1496:1506	arg1	kifunensine					1518:1528	mannosidase inhibitor kifunensine	1496:1528	mannosidase inhibitor kifunensine into the medium and cutting purified protein	1496:1573	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	7	62	theme	Necl-4	1056:1061	arg1	protein					1063:1069	Necl-4 protein	1056:1069	Necl-4 protein	1056:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	9	63	theme	modified	1350:1357	arg1	system					1391:1396	modified AP mammalian protein expression system	1350:1396	modified AP mammalian protein expression system	1350:1396	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	10	64	from	protein	1639:1645	arg1	quantities					1660:1669	milligram quantities	1650:1669	milligram quantities	1650:1669	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	8	65	theme	expression	1275:1284	arg1	level					1286:1290	an expression level	1272:1290	an expression level of 4 mg/L culture and purity of 95%	1272:1326	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	2	66	theme	extracellular	251:263	arg1	domain					265:270	the extracellular domain	247:270	the extracellular domain of Necls	247:279	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	2	66	theme	extracellular	251:263	arg1	Necls					275:279	Necls	275:279	Necls	275:279	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	9	67	theme	expression	1380:1389	arg1	system					1391:1396	modified AP mammalian protein expression system	1350:1396	modified AP mammalian protein expression system	1350:1396	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	1	68	theme	high	157:160	arg1	production					162:171	high production	157:171	high production of Nectin-like 4 (Necl-4) protein	157:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	7	69	with	reaction	1098:1105	arg1	AP-tag					1116:1121	the AP-tag	1112:1121	the AP-tag	1112:1121	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	4	70	theme	processing	598:607	arg1	kifunensine					619:629	N-glycosylation processing inhibitor kifunensine	582:629	N-glycosylation processing inhibitor kifunensine	582:629	Then, by adding N-glycosylation processing inhibitor kifunensine into the medium, complex glycan was inhibited to generate.					
29620509	5	71	theme	protein	722:728	arg1	glycan					703:708	The residual glycan	690:708	The residual glycan of purified protein	690:728	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	5	72	theme	rhinovirus	811:820	arg1	protease					822:829	human rhinovirus protease and size exclusion chromatography	805:863	protease	822:829	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	2	73	from	terminus	334:341	arg1	pAPtag					318:323	the modified vector pAPtag	298:323	the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression	298:392	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	8	74	theme	purified	1181:1188	arg1	protein					1197:1203	purified Necl-4 protein	1181:1203	purified Necl-4 protein	1181:1203	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	7	75	theme	cell	1021:1024	arg1	lines					1026:1030	The transient and stable cell lines	996:1030	Result The transient and stable cell lines with high production of Necl-4 protein	989:1069	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	11	76	gly	glycoprotein	1741:1752	arg1	glycoprotein					1741:1752	glycoprotein	1741:1752	glycoprotein	1741:1752	Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.					
29620509	8	77	theme	protein	1197:1203	arg1	form					1173:1176	The soluble and active form	1150:1176	The soluble and active form of purified Necl-4 protein	1150:1203	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	10	78	theme	purified	1558:1565	arg1	protein					1567:1573	cutting purified protein	1550:1573	cutting purified protein	1550:1573	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	10	79	theme	Necl-4	1632:1637	arg1	protein					1639:1645	deglycosylated Necl-4 protein	1617:1645	deglycosylated Necl-4 protein in milligram quantities	1617:1669	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	1	80	theme	Necl-4	191:196	arg1	protein					199:205	Nectin-like 4 (Necl-4) protein	176:205	Nectin-like 4 (Necl-4) protein	176:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	3	81	theme	activity	524:531	arg1	assay					533:537	the AP activity assay	517:537	the AP activity assay	517:537	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	6	82	theme	Necl-4	896:901	arg1	protein					903:909	purified Necl-4 protein	887:909	purified Necl-4 protein	887:909	The concentration of purified Necl-4 protein was monitored by measuring the absorbance at 280 nm and analyzed by SDS-PAGE.					
29620509	9	83	theme	high	1424:1427	arg1	lines					1452:1456	the high productive stable cell lines	1420:1456	the high productive stable cell lines	1420:1456	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	2	84	dep	cloned	286:291	arg1	First					216:220	First	216:220	First	216:220	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	2	85	theme	modified	302:309	arg1	pAPtag					318:323	the modified vector pAPtag	298:323	the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression	298:392	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	3	86	theme	blot	551:554	arg1	analysis					556:563	Western blot analysis	543:563	Western blot analysis	543:563	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	1	87	theme	stable	134:139	arg1	lines					146:150	the transient and stable cell lines	116:150	the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein	116:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	0	88	from	Purification	27:38	arg1	Line					90:93	293ET Cell Line	79:93	293ET Cell Line	79:93	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	8	89	theme	protein	1259:1265	arg1	deglycosylation					1224:1238	deglycosylation	1224:1238	deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%	1224:1326	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	0	90	theme	Cell	85:88	arg1	Line					90:93	293ET Cell Line	79:93	293ET Cell Line	79:93	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	1	91	with	lines	146:150	arg1	production					162:171	high production	157:171	high production of Nectin-like 4 (Necl-4) protein	157:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	5	92	theme	exclusion	840:848	arg1	chromatography					850:863	human rhinovirus protease and size exclusion chromatography	805:863	chromatography	850:863	The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography.					
29620509	0	93	from	Expression	12:21	arg1	Line					90:93	293ET Cell Line	79:93	293ET Cell Line	79:93	Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.					
29620509	10	94	theme	deglycosylated	1617:1630	arg1	protein					1639:1645	deglycosylated Necl-4 protein	1617:1645	deglycosylated Necl-4 protein in milligram quantities	1617:1669	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	9	95	theme	activity	1470:1477	arg1	assay					1479:1483	AP activity assay	1467:1483	AP activity assay	1467:1483	Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay.					
29620509	2	96	theme	phosphatase	355:365	arg1	terminus					334:341	the N terminus	328:341	the N terminus of alkaline phosphatase (AP)	328:370	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	10	97	gly	deglycosylated	1617:1630	arg1	protein					1639:1645	deglycosylated Necl-4 protein	1617:1645	deglycosylated Necl-4 protein in milligram quantities	1617:1669	By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities.					
29620509	8	98	theme	culture	1302:1308	arg1	level					1286:1290	an expression level	1272:1290	an expression level of 4 mg/L culture and purity of 95%	1272:1326	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	8	99	theme	purity	1314:1319	arg1	level					1286:1290	an expression level	1272:1290	an expression level of 4 mg/L culture and purity of 95%	1272:1326	The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%.					
29620509	7	100	theme	color	1092:1096	arg1	reaction					1098:1105	the color reaction	1088:1105	the color reaction with the AP-tag in the recombinant vector	1088:1147	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	7	101	theme	recombinant	1130:1140	arg1	vector					1142:1147	the recombinant vector	1126:1147	the recombinant vector	1126:1147	Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector.					
29620509	1	102	theme	cell	141:144	arg1	lines					146:150	the transient and stable cell lines	116:150	the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein	116:205	Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein.					
29620509	2	103	theme	vector	311:316	arg1	pAPtag					318:323	the modified vector pAPtag	298:323	the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression	298:392	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29620509	3	104	theme	fusion	439:444	arg1	protein					446:452	Necls-AP fusion protein	430:452	Necls-AP fusion protein	430:452	Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis.					
29620509	2	105	theme	N	332:332	arg1	terminus					334:341	the N terminus	328:341	the N terminus of alkaline phosphatase (AP)	328:370	Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression.					
29802926	0	0	theme	immunomodulatory	91:106	arg1	activity					108:115	potentially immunomodulatory activity	79:115	potentially immunomodulatory activity	79:115	Two heteropolysaccharides from Isaria cicadae Miquel differ in composition and potentially immunomodulatory activity.					
29802926	3	1	theme	acid	496:499	arg1	contents					464:471	higher contents	457:471	higher contents of sulfated and uronic acid	457:499	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	2	2	with	galactose	387:395	arg1	ratio					427:431	different monosaccharide ratio	402:431	different monosaccharide ratio	402:431	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	1	3	theme	molecular	173:181	arg1	weights					183:189	molecular weights	173:189	molecular weights of 30.9 and 555.3 kDa	173:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	7	4	theme	functional	1082:1091	arg1	food					1093:1096	a functional food	1080:1096	a functional food with the better immunomodulator activity	1080:1137	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	7	4	theme	functional	1082:1091	arg1	JCH-1					1045:1049	JCH-1	1045:1049	JCH-1	1045:1049	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	7	5	contain	had	981:983	arg2	fractions					1004:1012	two polysaccharide fractions	985:1012	two polysaccharide fractions	985:1012	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	7	5	contain	had	981:983	arg1	Miquel					974:979	Miquel	974:979	Miquel	974:979	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	6	6	theme	immunomodulatory	863:878	arg1	better					916:921	better	916:921	better	916:921	Furthermore, the immunomodulatory activity of JCH-1 was significantly better than that of JCH-2.					
29802926	6	6	theme	immunomodulatory	863:878	arg1	activity					880:887	the immunomodulatory activity	859:887	the immunomodulatory activity of JCH-1	859:896	Furthermore, the immunomodulatory activity of JCH-1 was significantly better than that of JCH-2.					
29802926	5	7	theme	NO	826:827	arg1	release					815:821	the release	811:821	the release of NO, IL-6 and TNF-α	811:843	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	4	8	theme	cells	609:613	arg1	proliferation					583:595	the proliferation	579:595	the proliferation of RAW264.7 cells	579:613	Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range.					
29802926	1	9	dep	heteropolysaccharides	128:148	arg1	JCH-1					151:155	JCH-1	151:155	JCH-1	151:155	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	1	9	dep	heteropolysaccharides	128:148	arg1	heteropolysaccharides					128:148	Two novel heteropolysaccharides	118:148	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa	118:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	1	9	dep	heteropolysaccharides	128:148	arg1	JCH-2					161:165	JCH-2	161:165	JCH-2	161:165	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	4	10	theme	specific	648:655	arg1	range					671:675	a specific concentration range	646:675	a specific concentration range	646:675	Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range.					
29802926	0	11	from	Miquel	46:51	arg1	heteropolysaccharides					4:24	Two heteropolysaccharides	0:24	Two heteropolysaccharides from Isaria cicadae Miquel	0:51	Two heteropolysaccharides from Isaria cicadae Miquel differ in composition and potentially immunomodulatory activity.					
29802926	5	12	theme	TNF-α	839:843	arg1	release					815:821	the release	811:821	the release of NO, IL-6 and TNF-α	811:843	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	1	13	with	heteropolysaccharides	128:148	arg1	weights					183:189	molecular weights	173:189	molecular weights of 30.9 and 555.3 kDa	173:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	1	14	theme	30.9	194:197	arg1	weights					183:189	molecular weights	173:189	molecular weights of 30.9 and 555.3 kDa	173:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	6	15	theme	JCH-1	892:896	arg1	better					916:921	better	916:921	better	916:921	Furthermore, the immunomodulatory activity of JCH-1 was significantly better than that of JCH-2.					
29802926	6	15	theme	JCH-1	892:896	arg1	activity					880:887	the immunomodulatory activity	859:887	the immunomodulatory activity of JCH-1	859:896	Furthermore, the immunomodulatory activity of JCH-1 was significantly better than that of JCH-2.					
29802926	5	16	theme	immunomodulatory	682:697	arg1	assay					699:703	The immunomodulatory assay	678:703	The immunomodulatory assay	678:703	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	2	17	theme	Monosaccharide	297:310	arg1	analysis					312:319	Monosaccharide analysis	297:319	Monosaccharide analysis	297:319	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	3	18	theme	higher	457:462	arg1	contents					464:471	higher contents	457:471	higher contents of sulfated and uronic acid	457:499	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	3	19	contain	had	453:455	arg2	contents					464:471	higher contents	457:471	higher contents of sulfated and uronic acid	457:499	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	3	19	contain	had	453:455	arg1	JCH-1					447:451	JCH-1	447:451	JCH-1	447:451	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	1	20	theme	novel	122:126	arg1	JCH-1					151:155	JCH-1	151:155	JCH-1	151:155	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	1	20	theme	novel	122:126	arg1	heteropolysaccharides					128:148	Two novel heteropolysaccharides	118:148	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa	118:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	1	20	theme	novel	122:126	arg1	JCH-2					161:165	JCH-2	161:165	JCH-2	161:165	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	2	21	theme	monosaccharide	412:425	arg1	ratio					427:431	different monosaccharide ratio	402:431	different monosaccharide ratio	402:431	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	2	22	theme	different	402:410	arg1	ratio					427:431	different monosaccharide ratio	402:431	different monosaccharide ratio	402:431	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	5	23	theme	macrophage	781:790	arg1	cells					792:796	macrophage cells	781:796	macrophage cells	781:796	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	7	24	theme	polysaccharide	989:1002	arg1	fractions					1004:1012	two polysaccharide fractions	985:1012	two polysaccharide fractions	985:1012	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	5	25	theme	IL-6	830:833	arg1	release					815:821	the release	811:821	the release of NO, IL-6 and TNF-α	811:843	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	5	26	theme	cells	792:796	arg1	viability					768:776	the viability	764:776	the viability of macrophage cells	764:796	The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α.					
29802926	7	27	theme	immunomodulator	1114:1128	arg1	activity					1130:1137	the better immunomodulator activity	1103:1137	the better immunomodulator activity	1103:1137	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	4	28	theme	MTT	529:531	arg1	assay					533:537	MTT assay	529:537	MTT assay	529:537	Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range.					
29802926	3	29	theme	uronic	489:494	arg1	acid					496:499	sulfated and uronic acid	476:499	sulfated and uronic acid	476:499	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	7	30	theme	different	1019:1027	arg1	composition					1029:1039	different composition	1019:1039	different composition	1019:1039	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	2	31	with	mannose	366:372	arg1	ratio					427:431	different monosaccharide ratio	402:431	different monosaccharide ratio	402:431	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	3	32	theme	sulfated	476:483	arg1	acid					496:499	sulfated and uronic acid	476:499	sulfated and uronic acid	476:499	In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2.					
29802926	7	33	with	food	1093:1096	arg1	activity					1130:1137	the better immunomodulator activity	1103:1137	the better immunomodulator activity	1103:1137	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	1	34	theme	555.3 kDa	203:211	arg1	weights					183:189	molecular weights	173:189	molecular weights of 30.9 and 555.3 kDa	173:211	Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel).					
29802926	4	35	theme	RAW264.7	600:607	arg1	cells					609:613	RAW264.7 cells	600:613	RAW264.7 cells	600:613	Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range.					
29802926	7	36	theme	better	1107:1112	arg1	activity					1130:1137	the better immunomodulator activity	1103:1137	the better immunomodulator activity	1103:1137	These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.					
29802926	2	37	with	glucose	375:381	arg1	ratio					427:431	different monosaccharide ratio	402:431	different monosaccharide ratio	402:431	Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio.					
29802926	4	38	theme	concentration	657:669	arg1	range					671:675	a specific concentration range	646:675	a specific concentration range	646:675	Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range.					
30412387	7	0	theme	modified	1152:1159	arg1	function					1172:1179	modified nanofibril function	1152:1179	modified nanofibril function as interfacial reinforcement and aggregation	1152:1224	The trade-off between modified nanofibril function as interfacial reinforcement and aggregation leads to an optimum loading.					
30412387	5	1	theme	phase	909:913	arg1	addition					881:888	addition	881:888	addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively)	881:977	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	2	theme	PVF	771:773	arg1	films					775:779	neat PVF films	766:779	neat PVF films	766:779	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	0	3	theme	Cellulose	61:69	arg1	Nanofiber					71:79	Modified Cellulose Nanofiber	52:79	Modified Cellulose Nanofiber Bearing UV Shielding Property	52:109	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	3	4	theme	X-ray	489:493	arg1	spectroscopies					509:522	X-ray photoelectron spectroscopies	489:522	X-ray photoelectron spectroscopies	489:522	A thorough characterization was carried out via elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR.					
30412387	6	5	located	observed	1046:1053	arg1	loadings					1107:1114	CNF loadings	1103:1114	CNF loadings below 5 wt %	1103:1127	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	6	5	located	observed	1046:1053	arg2	performance					999:1009	A high UV blocking performance	980:1009	A high UV blocking performance	980:1009	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	0	6	theme	UV	89:90	arg1	Property					102:109	UV Shielding Property	89:109	UV Shielding Property	89:109	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	5	7	theme	MPa	949:951	arg1	values					924:929	maximum values	916:929	maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively	916:976	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	8	dep	phase	909:913	arg1	values					924:929	maximum values	916:929	maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively	916:976	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	6	9	theme	wt	1124:1125	arg1	%					1127:1127	5 wt %	1122:1127	5 wt %	1122:1127	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	2	10	theme	TEMPO-oxidized	250:263	arg1	CNFs					265:268	TEMPO-oxidized CNFs	250:268	TEMPO-oxidized CNFs	250:268	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	5	11	theme	neat	766:769	arg1	films					775:779	neat PVF films	766:779	neat PVF films	766:779	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	12	theme	maximum	916:922	arg1	values					924:929	maximum values	916:929	maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively	916:976	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	13	theme	films	841:845	arg1	strength					794:801	the tensile strength	782:801	the tensile strength	782:801	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	13	theme	films	841:845	arg1	modulus					810:816	Young modulus	804:816	Young modulus	804:816	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	5	13	theme	films	841:845	arg1	elongation					823:832	elongation	823:832	elongation of the films	823:845	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	3	14	theme	thorough	403:410	arg1	characterization					412:427	A thorough characterization	401:427	A thorough characterization	401:427	A thorough characterization was carried out via elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR.					
30412387	0	15	theme	Shielding	92:100	arg1	Property					102:109	UV Shielding Property	89:109	UV Shielding Property	89:109	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	6	16	from	performance	999:1009	arg1	region					1034:1039	the UVB region	1026:1039	the UVB region	1026:1039	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	3	17	theme	solid-state	528:538	arg1	NMR					540:542	solid-state NMR	528:542	solid-state NMR	528:542	A thorough characterization was carried out via elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR.					
30412387	1	18	theme	cellulose	184:192	arg1	nanofibers					194:203	cellulose nanofibers	184:203	cellulose nanofibers (CNFs)	184:210	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	1	18	theme	cellulose	184:192	arg1	CNFs					206:209	CNFs	206:209	CNFs	206:209	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	0	19	theme	Transparent	7:17	arg1	Films					41:45	Highly Transparent, Strong, and Flexible Films	0:45	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.	0:110	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	5	20	theme	tensile	786:792	arg1	strength					794:801	the tensile strength	782:801	the tensile strength	782:801	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	7	21	theme	nanofibril	1161:1170	arg1	function					1172:1179	modified nanofibril function	1152:1179	modified nanofibril function as interfacial reinforcement and aggregation	1152:1224	The trade-off between modified nanofibril function as interfacial reinforcement and aggregation leads to an optimum loading.					
30412387	5	22	theme	MPa	938:940	arg1	values					924:929	maximum values	916:929	maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively	916:976	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	2	23	theme	soybean	359:365	arg1	oil					367:369	epoxidized soybean oil	348:369	epoxidized soybean oil	348:369	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	4	24	with	films	624:628	arg1	capabilities					648:659	UV-absorbing capabilities	635:659	UV-absorbing capabilities	635:659	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	5	25	theme	reinforcing	897:907	arg1	phase					909:913	the reinforcing phase	893:913	the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively)	893:977	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	2	26	theme	epoxidized	348:357	arg1	oil					367:369	epoxidized soybean oil	348:369	epoxidized soybean oil	348:369	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	0	27	theme	Strong	20:25	arg1	Films					41:45	Highly Transparent, Strong, and Flexible Films	0:45	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.	0:110	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	5	28	theme	∼	943:943	arg1	MPa					949:951	∼ 714 MPa	943:951	∼ 714 MPa	943:951	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	3	29	theme	elemental	449:457	arg1	analysis					459:466	elemental analysis	449:466	elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR	449:542	A thorough characterization was carried out via elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR.					
30412387	6	30	theme	CNF	1103:1105	arg1	loadings					1107:1114	CNF loadings	1103:1114	CNF loadings below 5 wt %	1103:1127	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	4	31	theme	surface-modified	560:575	arg1	CNFs					577:580	the surface-modified CNFs	556:580	the surface-modified CNFs	556:580	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	2	32	with	phase	305:309	arg1	oil					367:369	epoxidized soybean oil	348:369	epoxidized soybean oil	348:369	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	2	32	with	phase	305:309	arg1	diisocyanate					330:341	diisocyanate	330:341	diisocyanate	330:341	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	2	32	with	phase	305:309	arg1	benzophenone					316:327	benzophenone	316:327	benzophenone	316:327	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	1	33	theme	multifunctional	135:149	arg1	films					161:165	multifunctional composite films	135:165	multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA)	135:240	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	4	34	theme	thermomechanical	684:699	arg1	strength					701:708	their thermomechanical strength	678:708	their thermomechanical strength	678:708	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	6	35	theme	multifunctional	1074:1088	arg1	films					1094:1098	the introduced multifunctional PVA films	1059:1098	the introduced multifunctional PVA films	1059:1098	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	5	36	theme	dramatic	857:864	arg1	increases					866:874	dramatic increases	857:874	dramatic increases	857:874	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	2	37	theme	esterification	375:388	arg1	reactions					390:398	esterification reactions	375:398	esterification reactions	375:398	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	5	38	theme	Young	804:808	arg1	modulus					810:816	Young modulus	804:816	Young modulus	804:816	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	6	39	theme	blocking	990:997	arg1	performance					999:1009	A high UV blocking performance	980:1009	A high UV blocking performance	980:1009	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	8	40	theme	active	1316:1321	arg1	packaging					1323:1331	active packaging	1316:1331	active packaging	1316:1331	The results indicate promising applications, for example, in active packaging.					
30412387	4	41	theme	light	733:737	arg1	transmittance					739:751	a high light transmittance	726:751	a high light transmittance	726:751	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	0	42	theme	Flexible	32:39	arg1	Films					41:45	Highly Transparent, Strong, and Flexible Films	0:45	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.	0:110	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	4	43	theme	high	728:731	arg1	transmittance					739:751	a high light transmittance	726:751	a high light transmittance	726:751	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	5	44	theme	%	962:962	arg1	values					924:929	maximum values	916:929	maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively	916:976	Compared to neat PVF films, the tensile strength, Young modulus, and elongation of the films underwent dramatic increases upon addition of the reinforcing phase (maximum values of ∼96 MPa, ∼ 714 MPa, and ∼350%, respectively).					
30412387	6	45	theme	high	982:985	arg1	performance					999:1009	A high UV blocking performance	980:1009	A high UV blocking performance	980:1009	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	6	46	theme	introduced	1063:1072	arg1	films					1094:1098	the introduced multifunctional PVA films	1059:1098	the introduced multifunctional PVA films	1059:1098	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	6	47	theme	UV	987:988	arg1	performance					999:1009	A high UV blocking performance	980:1009	A high UV blocking performance	980:1009	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	8	48	theme	promising	1276:1284	arg1	applications					1286:1297	promising applications	1276:1297	promising applications	1276:1297	The results indicate promising applications, for example, in active packaging.					
30412387	0	49	with	Films	41:45	arg1	Nanofiber					71:79	Modified Cellulose Nanofiber	52:79	Modified Cellulose Nanofiber Bearing UV Shielding Property	52:109	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	2	50	theme	heterogeneous	291:303	arg1	phase					305:309	the heterogeneous phase	287:309	the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions	287:398	First, TEMPO-oxidized CNFs were modified in the heterogeneous phase with benzophenone, diisocyanate, and epoxidized soybean oil via esterification reactions.					
30412387	6	51	theme	PVA	1090:1092	arg1	films					1094:1098	the introduced multifunctional PVA films	1059:1098	the introduced multifunctional PVA films	1059:1098	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	1	52	theme	vinyl	221:225	arg1	alcohol					227:233	vinyl alcohol	221:233	vinyl alcohol	221:233	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	1	52	theme	vinyl	221:225	arg1	poly					216:219	poly	216:219	poly(vinyl alcohol) (PVA)	216:240	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	0	53	theme	Modified	52:59	arg1	Nanofiber					71:79	Modified Cellulose Nanofiber	52:79	Modified Cellulose Nanofiber Bearing UV Shielding Property	52:109	Highly Transparent, Strong, and Flexible Films with Modified Cellulose Nanofiber Bearing UV Shielding Property.					
30412387	4	54	theme	UV-absorbing	635:646	arg1	capabilities					648:659	UV-absorbing capabilities	635:659	UV-absorbing capabilities	635:659	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	3	55	theme	photoelectron	495:507	arg1	spectroscopies					509:522	X-ray photoelectron spectroscopies	489:522	X-ray photoelectron spectroscopies	489:522	A thorough characterization was carried out via elemental analysis as well as FT-IR and X-ray photoelectron spectroscopies and solid-state NMR.					
30412387	6	56	theme	UVB	1030:1032	arg1	region					1034:1039	the UVB region	1026:1039	the UVB region	1026:1039	A high UV blocking performance, especially in the UVB region, was observed for the introduced multifunctional PVA films at CNF loadings below 5 wt %.					
30412387	4	57	theme	composite	614:622	arg1	films					624:628	composite films	614:628	composite films with UV-absorbing capabilities	614:659	Following, the surface-modified CNFs were combined with PVA to endow composite films with UV-absorbing capabilities while increasing their thermomechanical strength and maintaining a high light transmittance.					
30412387	7	58	theme	optimum	1238:1244	arg1	loading					1246:1252	an optimum loading	1235:1252	an optimum loading	1235:1252	The trade-off between modified nanofibril function as interfacial reinforcement and aggregation leads to an optimum loading.					
30412387	1	59	theme	composite	151:159	arg1	films					161:165	multifunctional composite films	135:165	multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA)	135:240	This work investigates multifunctional composite films synthesized with cellulose nanofibers (CNFs) and poly(vinyl alcohol) (PVA).					
30412387	7	60	theme	interfacial	1184:1194	arg1	reinforcement					1196:1208	interfacial reinforcement	1184:1208	interfacial reinforcement	1184:1208	The trade-off between modified nanofibril function as interfacial reinforcement and aggregation leads to an optimum loading.					
30353291	3	0	theme	further	543:549	arg1	analysis					551:558	further analysis	543:558	further analysis of the in-ESI HDX methodology	543:588	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	6	1	theme	solvated	945:952	arg1	species					954:960	solvated species	945:960	solvated species	945:960	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	3	2	theme	methodology	578:588	arg1	analysis					551:558	further analysis	543:558	further analysis of the in-ESI HDX methodology	543:588	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	9	3	theme	experimental	1456:1467	arg1	results					1469:1475	our experimental results	1452:1475	our experimental results	1452:1475	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	3	4	theme	in-ESI	567:572	arg1	methodology					578:588	the in-ESI HDX methodology	563:588	the in-ESI HDX methodology	563:588	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	1	5	theme	biological	185:194	arg1	functions					196:204	their biological functions	179:204	their biological functions	179:204	The conformations of glycans are crucial for their biological functions.					
30353291	10	6	theme	fundamental	1617:1627	arg1	processes					1629:1637	the fundamental processes	1613:1637	the fundamental processes of ESI	1613:1644	In total, this work illustrates how the fundamental processes of ESI alter the magnitude of HDX for carbohydrates and suggest parameters that should be considered and/or optimized prior to performing experiments with this in-ESI HDX technique.					
30353291	7	7	theme	infusion	1116:1123	arg1	rate					1125:1128	sample infusion rate	1109:1128	sample infusion rate	1109:1128	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	4	8	theme	analyte	652:658	arg1	transitioning					660:672	the analyte transitioning	648:672	the analyte transitioning from solution to gas-phase ions	648:704	For instance, in this method, HDX occurs concurrently to the analyte transitioning from solution to gas-phase ions.					
30353291	11	9	theme	Graphical	1821:1829	arg1	Abstract					1831:1838	Graphical Abstract	1821:1838	Graphical Abstract	1821:1838	Graphical Abstract ᅟ.					
30353291	10	10	theme	in-ESI	1799:1804	arg1	technique					1810:1818	this in-ESI HDX technique	1794:1818	this in-ESI HDX technique	1794:1818	In total, this work illustrates how the fundamental processes of ESI alter the magnitude of HDX for carbohydrates and suggest parameters that should be considered and/or optimized prior to performing experiments with this in-ESI HDX technique.					
30353291	9	11	theme	individual	1499:1508	arg1	parameter					1514:1522	an individual ESI parameter	1496:1522	an individual ESI parameter	1496:1522	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	7	12	theme	flow	1098:1101	arg1	rate					1103:1106	sheath gas flow rate	1087:1106	sheath gas flow rate	1087:1106	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	2	13	theme	exchange-mass	259:271	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	13	theme	exchange-mass	259:271	arg1	HDX-MS					287:292	HDX-MS	287:292	HDX-MS	287:292	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	13	theme	exchange-mass	259:271	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	7	14	theme	auxiliary	1062:1070	arg1	rate					1081:1084	auxiliary gas flow rate	1062:1084	auxiliary gas flow rate	1062:1084	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	15	theme	sheath	1087:1092	arg1	rate					1103:1106	sheath gas flow rate	1087:1106	sheath gas flow rate	1087:1106	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	8	16	from	changes	1246:1252	arg1	solvents					1348:1355	solvents	1348:1355	solvents	1348:1355	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	7	17	theme	spray	1035:1039	arg1	composition					1049:1059	spray solvent composition	1035:1059	spray solvent composition	1035:1059	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	8	18	theme	hydrogen	1328:1335	arg1	bonding					1337:1343	its intramolecular and intermolecular hydrogen bonding	1290:1343	its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1290:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	0	19	theme	Electrospray	20:31	arg1	ESI					45:47	ESI	45:47	ESI	45:47	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	0	19	theme	Electrospray	20:31	arg1	Ionization					33:42	Electrospray Ionization	20:42	Electrospray Ionization (ESI)	20:48	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	0	20	theme	Hydrogen/Deuterium	71:88	arg1	Exchange					90:97	In-ESI Hydrogen/Deuterium Exchange	64:97	In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts	64:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	7	21	theme	ESI	1009:1011	arg1	concentration					1138:1150	sample concentration	1131:1150	sample concentration	1131:1150	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	rate					1081:1084	auxiliary gas flow rate	1062:1084	auxiliary gas flow rate	1062:1084	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	composition					1049:1059	spray solvent composition	1035:1059	spray solvent composition	1035:1059	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	parameters					1013:1022	ESI parameters	1009:1022	ESI parameters	1009:1022	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	rate					1125:1128	sample infusion rate	1109:1128	sample infusion rate	1109:1128	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	voltage					1163:1169	spray voltage	1157:1169	spray voltage	1157:1169	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	21	theme	ESI	1009:1011	arg1	rate					1103:1106	sheath gas flow rate	1087:1106	sheath gas flow rate	1087:1106	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	9	22	theme	in-ESI	1565:1570	arg1	HDX					1572:1574	in-ESI HDX	1565:1574	in-ESI HDX	1565:1574	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	2	23	dep	hydroxyls	410:418	arg1	e.g.					404:407	e.g.	404:407	e.g.	404:407	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	0	24	theme	Ion	121:123	arg1	Adducts					125:131	Carbohydrate-Metal Ion Adducts	102:131	Carbohydrate-Metal Ion Adducts	102:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	0	25	theme	In-ESI	64:69	arg1	Exchange					90:97	In-ESI Hydrogen/Deuterium Exchange	64:97	In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts	64:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	8	26	theme	methanol	1388:1395	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	8	27	theme	water	1401:1405	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	7	28	theme	solvent	1041:1047	arg1	composition					1049:1059	spray solvent composition	1035:1059	spray solvent composition	1035:1059	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	3	29	theme	regular	470:476	arg1	application					478:488	regular application	470:488	regular application of in-ESI HDX to characterize carbohydrates	470:532	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	8	30	with	bonding	1337:1343	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	2	31	theme	exchanging	374:383	arg1	groups					396:401	rapidly exchanging functional groups	366:401	rapidly exchanging functional groups	366:401	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	6	32	theme	metal-adducted	856:869	arg1	carbohydrates					871:883	metal-adducted carbohydrates	856:883	metal-adducted carbohydrates from gas-phase HDX	856:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	2	33	theme	In-electrospray	207:221	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	33	theme	In-electrospray	207:221	arg1	HDX-MS					287:292	HDX-MS	287:292	HDX-MS	287:292	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	33	theme	In-electrospray	207:221	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	8	34	from	solvents	1348:1355	arg1	changes					1246:1252	the structural changes	1231:1252	the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1231:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	9	35	theme	molecular	1414:1422	arg1	simulations					1432:1442	These molecular dynamic simulations	1408:1442	These molecular dynamic simulations	1408:1442	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	7	36	theme	in-ESI	1201:1206	arg1	HDX					1208:1210	in-ESI HDX	1201:1210	in-ESI HDX	1201:1210	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	37	theme	sample	1131:1136	arg1	concentration					1138:1150	sample concentration	1131:1150	sample concentration	1131:1150	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	6	38	theme	in-ESI	842:847	arg1	HDX					849:851	in-ESI HDX	842:851	in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX	842:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	3	39	theme	HDX	574:576	arg1	methodology					578:588	the in-ESI HDX methodology	563:588	the in-ESI HDX methodology	563:588	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	8	40	with	melezitose	1274:1283	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	2	41	theme	promising	300:308	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	41	theme	promising	300:308	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	8	42	theme	structural	1235:1244	arg1	changes					1246:1252	the structural changes	1231:1252	the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1231:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	6	43	from	HDX	900:902	arg1	carbohydrates					871:883	metal-adducted carbohydrates	856:883	metal-adducted carbohydrates from gas-phase HDX	856:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	6	43	from	HDX	900:902	arg1	HDX					849:851	in-ESI HDX	842:851	in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX	842:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	8	44	with	trisaccharide	1259:1271	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	7	45	theme	sample	1109:1114	arg1	rate					1125:1128	sample infusion rate	1109:1128	sample infusion rate	1109:1128	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	4	46	from	solution	679:686	arg1	transitioning					660:672	the analyte transitioning	648:672	the analyte transitioning from solution to gas-phase ions	648:704	For instance, in this method, HDX occurs concurrently to the analyte transitioning from solution to gas-phase ions.					
30353291	4	47	theme	gas-phase	691:699	arg1	ions					701:704	gas-phase ions	691:704	gas-phase ions	691:704	For instance, in this method, HDX occurs concurrently to the analyte transitioning from solution to gas-phase ions.					
30353291	2	48	theme	carbohydrate	333:344	arg1	conformations					346:358	carbohydrate conformations	333:358	carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	333:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	8	49	theme	trisaccharide	1259:1271	arg1	changes					1246:1252	the structural changes	1231:1252	the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1231:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	7	50	theme	gas	1094:1096	arg1	rate					1103:1106	sheath gas flow rate	1087:1106	sheath gas flow rate	1087:1106	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	9	51	theme	ESI	1510:1512	arg1	parameter					1514:1522	an individual ESI parameter	1496:1522	an individual ESI parameter	1496:1522	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	8	52	theme	melezitose	1274:1283	arg1	changes					1246:1252	the structural changes	1231:1252	the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1231:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	10	53	theme	ESI	1642:1644	arg1	processes					1629:1637	the fundamental processes	1613:1637	the fundamental processes of ESI	1613:1644	In total, this work illustrates how the fundamental processes of ESI alter the magnitude of HDX for carbohydrates and suggest parameters that should be considered and/or optimized prior to performing experiments with this in-ESI HDX technique.					
30353291	2	54	theme	hydrogen/deuterium	240:257	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	54	theme	hydrogen/deuterium	240:257	arg1	HDX-MS					287:292	HDX-MS	287:292	HDX-MS	287:292	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	54	theme	hydrogen/deuterium	240:257	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	7	55	theme	gas	1072:1074	arg1	rate					1081:1084	auxiliary gas flow rate	1062:1084	auxiliary gas flow rate	1062:1084	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	7	56	theme	flow	1076:1079	arg1	rate					1081:1084	auxiliary gas flow rate	1062:1084	auxiliary gas flow rate	1062:1084	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	10	57	theme	HDX	1806:1808	arg1	technique					1810:1818	this in-ESI HDX technique	1794:1818	this in-ESI HDX technique	1794:1818	In total, this work illustrates how the fundamental processes of ESI alter the magnitude of HDX for carbohydrates and suggest parameters that should be considered and/or optimized prior to performing experiments with this in-ESI HDX technique.					
30353291	8	58	theme	intramolecular	1294:1307	arg1	bonding					1337:1343	its intramolecular and intermolecular hydrogen bonding	1290:1343	its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1290:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	3	59	theme	HDX	500:502	arg1	application					478:488	regular application	470:488	regular application of in-ESI HDX to characterize carbohydrates	470:532	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	5	60	theme	solution-phase	772:785	arg1	conformations					787:799	both gas-phase and solution-phase conformations	753:799	both gas-phase and solution-phase conformations of the analyte	753:814	Therefore, there is a possibility of sampling both gas-phase and solution-phase conformations of the analyte.					
30353291	8	61	from	melezitose	1274:1283	arg1	solvents					1348:1355	solvents	1348:1355	solvents	1348:1355	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	1	62	theme	glycans	155:161	arg1	conformations					138:150	The conformations	134:150	The conformations of glycans	134:161	The conformations of glycans are crucial for their biological functions.					
30353291	1	62	theme	glycans	155:161	arg1	crucial					167:173	crucial	167:173	crucial	167:173	The conformations of glycans are crucial for their biological functions.					
30353291	8	63	theme	intermolecular	1313:1326	arg1	bonding					1337:1343	its intramolecular and intermolecular hydrogen bonding	1290:1343	its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1290:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	8	64	from	trisaccharide	1259:1271	arg1	solvents					1348:1355	solvents	1348:1355	solvents	1348:1355	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	8	65	theme	bonding	1337:1343	arg1	changes					1246:1252	the structural changes	1231:1252	the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water	1231:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	0	66	theme	Carbohydrate-Metal	102:119	arg1	Adducts					125:131	Carbohydrate-Metal Ion Adducts	102:131	Carbohydrate-Metal Ion Adducts	102:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	7	67	theme	parameters	1013:1022	arg1	effects					998:1004	the effects	994:1004	the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage,	994:1170	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	0	68	from	Parameters	50:59	arg1	Exchange					90:97	In-ESI Hydrogen/Deuterium Exchange	64:97	In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts	64:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	9	69	dep	conformations	1538:1550	arg1	sample					1555:1560	sample	1555:1560	sample	1555:1560	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	9	69	dep	conformations	1538:1550	arg1	we					1552:1553	we	1552:1553	we	1552:1553	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
30353291	0	70	theme	Adducts	125:131	arg1	Exchange					90:97	In-ESI Hydrogen/Deuterium Exchange	64:97	In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts	64:131	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	7	71	from	effects	1190:1196	arg1	HDX					1208:1210	in-ESI HDX	1201:1210	in-ESI HDX	1201:1210	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	8	72	theme	different	1362:1370	arg1	compositions					1372:1383	different compositions	1362:1383	different compositions of methanol and water	1362:1405	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	10	73	theme	HDX	1669:1671	arg1	magnitude					1656:1664	the magnitude	1652:1664	the magnitude of HDX for carbohydrates	1652:1689	In total, this work illustrates how the fundamental processes of ESI alter the magnitude of HDX for carbohydrates and suggest parameters that should be considered and/or optimized prior to performing experiments with this in-ESI HDX technique.					
30353291	8	74	from	bonding	1337:1343	arg1	solvents					1348:1355	solvents	1348:1355	solvents	1348:1355	Further, we model the structural changes of a trisaccharide, melezitose, and its intramolecular and intermolecular hydrogen bonding in solvents with different compositions of methanol and water.					
30353291	6	75	from	HDX	849:851	arg1	HDX					900:902	gas-phase HDX	890:902	gas-phase HDX	890:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	0	76	theme	Ionization	33:42	arg1	Characterization					0:15	Characterization	0:15	Characterization of Electrospray Ionization (ESI)	0:48	Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts.					
30353291	2	77	theme	ESI	456:458	arg1	timeframe					443:451	the timeframe	439:451	the timeframe of ESI	439:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	3	78	theme	in-ESI	493:498	arg1	HDX					500:502	in-ESI HDX	493:502	in-ESI HDX	493:502	However, regular application of in-ESI HDX to characterize carbohydrates requires further analysis of the in-ESI HDX methodology.					
30353291	5	79	theme	analyte	808:814	arg1	conformations					787:799	both gas-phase and solution-phase conformations	753:799	both gas-phase and solution-phase conformations of the analyte	753:814	Therefore, there is a possibility of sampling both gas-phase and solution-phase conformations of the analyte.					
30353291	7	80	theme	spray	1157:1161	arg1	voltage					1163:1169	spray voltage	1157:1169	spray voltage	1157:1169	We also systematically examine the effects of ESI parameters, including spray solvent composition, auxiliary gas flow rate, sheath gas flow rate, sample infusion rate, sample concentration, and spray voltage, and discuss their effects on in-ESI HDX.					
30353291	6	81	theme	gas-phase	890:898	arg1	HDX					900:902	gas-phase HDX	890:902	gas-phase HDX	890:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	2	82	theme	ESI	235:237	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	82	theme	ESI	235:237	arg1	HDX-MS					287:292	HDX-MS	287:292	HDX-MS	287:292	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	82	theme	ESI	235:237	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	83	theme	functional	385:394	arg1	groups					396:401	rapidly exchanging functional groups	366:401	rapidly exchanging functional groups	366:401	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	6	84	theme	carbohydrates	871:883	arg1	HDX					849:851	in-ESI HDX	842:851	in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX	842:902	Herein, we differentiate in-ESI HDX of metal-adducted carbohydrates from gas-phase HDX and illustrate that this method analyzes solvated species.					
30353291	5	85	theme	gas-phase	758:766	arg1	conformations					787:799	both gas-phase and solution-phase conformations	753:799	both gas-phase and solution-phase conformations of the analyte	753:814	Therefore, there is a possibility of sampling both gas-phase and solution-phase conformations of the analyte.					
30353291	2	86	theme	ionization	223:232	arg1	technique					310:318	a promising technique	298:318	a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI	298:458	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	86	theme	ionization	223:232	arg1	HDX-MS					287:292	HDX-MS	287:292	HDX-MS	287:292	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	2	86	theme	ionization	223:232	arg1	spectrometry					273:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry	207:284	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS)	207:293	In-electrospray ionization (ESI) hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a promising technique for studying carbohydrate conformations since rapidly exchanging functional groups, e.g., hydroxyls, can be labeled on the timeframe of ESI.					
30353291	9	87	theme	dynamic	1424:1430	arg1	simulations					1432:1442	These molecular dynamic simulations	1408:1442	These molecular dynamic simulations	1408:1442	These molecular dynamic simulations support our experimental results and illustrate how an individual ESI parameter can alter the conformations we sample by in-ESI HDX.					
31602930	0	0	theme	different	93:101	arg1	areas					114:118	different production areas	93:118	different production areas	93:118	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	1	1	theme	polysaccharide	369:382	arg1	content					384:390	polysaccharide content	369:390	polysaccharide content of P. umbellatus	369:407	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	3	2	from	province	837:844	arg1	difference					687:696	a certain difference	677:696	a certain difference in total polysaccharide content of P. umbellatus from different regions	677:768	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	2	from	province	837:844	arg1	polysaccharide					809:822	total P. umbellatus polysaccharide	789:822	total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	789:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	2	from	province	837:844	arg1	content					778:784	the content	774:784	the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	774:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	12	3	theme	analysis	1371:1378	arg1	results					1314:1320	the results	1310:1320	the results of cluster analysis( CA) and principal component analysis( PCA)	1310:1384	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	13	4	theme	polysaccharides	1774:1788	arg1	sequence					1693:1700	the sequence	1689:1700	the sequence of monosaccharide linking	1689:1726	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	13	4	theme	polysaccharides	1774:1788	arg1	activity					1748:1755	pharmacological activity	1732:1755	pharmacological activity of P. umbellatus polysaccharides	1732:1788	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	11	5	theme	galacturonic	1244:1255	arg1	acid					1257:1260	galacturonic acid	1244:1260	galacturonic acid	1244:1260	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	13	6	theme	umbellatus	1651:1660	arg1	resources					1662:1670	P. umbellatus resources	1648:1670	P. umbellatus resources	1648:1670	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	1	7	theme	exploitation	164:175	arg1	application					192:202	exploitation and sufficient application	164:202	exploitation and sufficient application of Polyporus umbellatus resources	164:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	1	8	theme	P.	282:283	arg1	polysaccharides					296:310	P. umbellatus polysaccharides	282:310	P. umbellatus polysaccharides	282:310	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	13	9	theme	linking	1720:1726	arg1	monosaccharide					1705:1718	monosaccharide linking	1705:1726	monosaccharide linking	1705:1726	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	0	10	from	[Study	0:5	arg1	content					25:31	polysaccharide content	10:31	polysaccharide content	10:31	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	0	10	from	[Study	0:5	arg1	composition					52:62	monosaccharide composition	37:62	monosaccharide composition	37:62	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	7	11	theme	glucose	1120:1126	arg1	contents					1108:1115	The contents	1104:1115	The contents of glucose( 17	1104:1130	The contents of glucose( 17.					
31602930	13	12	theme	P.	1760:1761	arg1	polysaccharides					1774:1788	P. umbellatus polysaccharides	1760:1788	P. umbellatus polysaccharides	1760:1788	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	1	13	theme	umbellatus	285:294	arg1	polysaccharides					296:310	P. umbellatus polysaccharides	282:310	P. umbellatus polysaccharides	282:310	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	0	14	theme	production	103:112	arg1	areas					114:118	different production areas	93:118	different production areas	93:118	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	3	15	theme	total	789:793	arg1	polysaccharide					809:822	total P. umbellatus polysaccharide	789:822	total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	789:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	13	16	theme	resources	1662:1670	arg1	utilization					1633:1643	rational utilization	1624:1643	rational utilization of P. umbellatus resources	1624:1670	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	13	17	theme	monosaccharide	1705:1718	arg1	sequence					1693:1700	the sequence	1689:1700	the sequence of monosaccharide linking	1689:1726	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	13	17	theme	monosaccharide	1705:1718	arg1	activity					1748:1755	pharmacological activity	1732:1755	pharmacological activity of P. umbellatus polysaccharides	1732:1788	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	1	18	theme	polysaccharides	296:310	arg1	composition					267:277	the monosaccharide composition	248:277	the monosaccharide composition of P. umbellatus polysaccharides	248:310	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	12	19	theme	component	1361:1369	arg1	PCA					1381:1383	PCA	1381:1383	PCA	1381:1383	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	12	19	theme	component	1361:1369	arg1	analysis					1371:1378	principal component analysis	1351:1378	principal component analysis( PCA)	1351:1384	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	1	20	theme	umbellatus	398:407	arg1	content					384:390	polysaccharide content	369:390	polysaccharide content of P. umbellatus	369:407	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	12	21	theme	principal	1351:1359	arg1	PCA					1381:1383	PCA	1381:1383	PCA	1381:1383	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	12	21	theme	principal	1351:1359	arg1	analysis					1371:1378	principal component analysis	1351:1378	principal component analysis( PCA)	1351:1384	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	3	22	from	regions	762:768	arg1	difference					687:696	a certain difference	677:696	a certain difference in total polysaccharide content of P. umbellatus from different regions	677:768	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	22	from	regions	762:768	arg1	content					778:784	the content	774:784	the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	774:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	23	from	difference	687:696	arg1	content					722:728	total polysaccharide content	701:728	total polysaccharide content of P. umbellatus	701:745	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	23	from	difference	687:696	arg1	province					837:844	province	837:844	province	837:844	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	1	24	theme	sufficient	181:190	arg1	application					192:202	exploitation and sufficient application	164:202	exploitation and sufficient application of Polyporus umbellatus resources	164:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	3	25	from	content	778:784	arg1	content					722:728	total polysaccharide content	701:728	total polysaccharide content of P. umbellatus	701:745	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	25	from	content	778:784	arg1	province					837:844	province	837:844	province	837:844	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	26	theme	polysaccharide	809:822	arg1	difference					687:696	a certain difference	677:696	a certain difference in total polysaccharide content of P. umbellatus from different regions	677:768	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	26	theme	polysaccharide	809:822	arg1	content					778:784	the content	774:784	the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	774:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	13	27	theme	rational	1624:1631	arg1	utilization					1633:1643	rational utilization	1624:1643	rational utilization of P. umbellatus resources	1624:1670	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	12	28	theme	cluster	1325:1331	arg1	analysis					1333:1340	cluster analysis	1325:1340	cluster analysis( CA)	1325:1345	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	12	28	theme	cluster	1325:1331	arg1	CA					1343:1344	CA	1343:1344	CA	1343:1344	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	3	29	theme	P.	795:796	arg1	polysaccharide					809:822	total P. umbellatus polysaccharide	789:822	total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1	789:868	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	13	30	theme	P.	1648:1649	arg1	resources					1662:1670	P. umbellatus resources	1648:1670	P. umbellatus resources	1648:1670	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	13	31	theme	pharmacological	1732:1746	arg1	activity					1748:1755	pharmacological activity	1732:1755	pharmacological activity of P. umbellatus polysaccharides	1732:1788	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	1	32	theme	Polyporus	207:215	arg1	resources					228:236	Polyporus umbellatus resources	207:236	Polyporus umbellatus resources	207:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	0	33	theme	polysaccharide	10:23	arg1	content					25:31	polysaccharide content	10:31	polysaccharide content	10:31	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	1	34	theme	anthrone-sulfuric	316:332	arg1	method					339:344	the anthrone-sulfuric acid method	312:344	the anthrone-sulfuric acid method	312:344	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	1	35	theme	producing	417:425	arg1	areas					427:431	17 producing areas	414:431	17 producing areas	414:431	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	12	36	theme	analysis	1333:1340	arg1	results					1314:1320	the results	1310:1320	the results of cluster analysis( CA) and principal component analysis( PCA)	1310:1384	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	1	37	theme	umbellatus	217:226	arg1	resources					228:236	Polyporus umbellatus resources	207:236	Polyporus umbellatus resources	207:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	0	38	theme	monosaccharide	37:50	arg1	composition					52:62	monosaccharide composition	37:62	monosaccharide composition	37:62	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	1	39	theme	acid	334:337	arg1	method					339:344	the anthrone-sulfuric acid method	312:344	the anthrone-sulfuric acid method	312:344	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	2	40	theme	component	596:604	arg1	content					565:571	the content	561:571	the content of each monosaccharide component	561:604	The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously.					
31602930	1	41	theme	resources	228:236	arg1	application					192:202	exploitation and sufficient application	164:202	exploitation and sufficient application of Polyporus umbellatus resources	164:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	2	42	theme	monosaccharide	581:594	arg1	component					596:604	each monosaccharide component	576:604	each monosaccharide component	576:604	The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously.					
31602930	3	43	theme	total	701:705	arg1	content					722:728	total polysaccharide content	701:728	total polysaccharide content of P. umbellatus	701:745	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	12	44	theme	Heilongjiang	1526:1537	arg1	province					1539:1546	Heilongjiang province	1526:1546	Heilongjiang province	1526:1546	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	3	45	theme	polysaccharide	707:720	arg1	content					722:728	total polysaccharide content	701:728	total polysaccharide content of P. umbellatus	701:745	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	13	46	theme	umbellatus	1763:1772	arg1	polysaccharides					1774:1788	P. umbellatus polysaccharides	1760:1788	P. umbellatus polysaccharides	1760:1788	The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.					
31602930	11	47	located	detected	1272:1279	arg2	fructose					1222:1229	fructose	1222:1229	fructose	1222:1229	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	11	47	located	detected	1272:1279	arg1	addition					1213:1220	addition	1213:1220	addition	1213:1220	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	11	47	located	detected	1272:1279	arg2	acid					1257:1260	galacturonic acid	1244:1260	galacturonic acid	1244:1260	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	11	47	located	detected	1272:1279	arg2	rhamnose					1231:1238	rhamnose	1231:1238	rhamnose	1231:1238	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	11	47	located	detected	1272:1279	arg1	samples					1289:1295	some samples	1284:1295	some samples	1284:1295	In addition,fructose,rhamnose and galacturonic acid were also detected in some samples.					
31602930	12	48	theme	polysaccharide	1437:1450	arg1	batches					1412:1418	totally 17 batches	1401:1418	totally 17 batches of P. umbellatus polysaccharide	1401:1450	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	3	49	dep	P.	795:796	arg1	umbellatus					798:807	umbellatus	798:807	umbellatus	798:807	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	3	50	theme	different	752:760	arg1	regions					762:768	different regions	752:768	different regions	752:768	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	12	51	dep	P.	1423:1424	arg1	umbellatus					1426:1435	umbellatus	1426:1435	umbellatus	1426:1435	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	0	52	from	areas	114:118	arg1	[Study					0:5	[Study	0:5	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas	0:118	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	12	53	theme	P.	1423:1424	arg1	polysaccharide					1437:1450	P. umbellatus polysaccharide	1423:1450	P. umbellatus polysaccharide	1423:1450	Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately.					
31602930	0	54	theme	umbellatus	77:86	arg1	[Study					0:5	[Study	0:5	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas	0:118	[Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas].					
31602930	3	55	theme	umbellatus	736:745	arg1	content					722:728	total polysaccharide content	701:728	total polysaccharide content of P. umbellatus	701:745	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	5	56	theme	90	882:883	arg1	%					884:884	%	884:884	%	884:884	90%) was relatively higher than that of others areas.					
31602930	1	57	theme	scientific	142:151	arg1	basics					153:158	scientific basics	142:158	scientific basics for exploitation and sufficient application of Polyporus umbellatus resources	142:236	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	6	58	theme	glucuronic	1034:1043	arg1	acid					1045:1048	glucuronic acid	1034:1048	glucuronic acid	1034:1048	Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose.					
31602930	1	59	theme	monosaccharide	252:265	arg1	composition					267:277	the monosaccharide composition	248:277	the monosaccharide composition of P. umbellatus polysaccharides	248:310	In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas.					
31602930	8	60	theme	65	1133:1134	arg1	mg·g-1					1136:1141	mg·g-1	1136:1141	mg·g-1	1136:1141	65 mg·g-1) was higher than others.					
31602930	3	61	theme	certain	679:685	arg1	difference					687:696	a certain difference	677:696	a certain difference in total polysaccharide content of P. umbellatus from different regions	677:768	The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1.					
31602930	5	62	theme	others	922:927	arg1	areas					929:933	others areas	922:933	others areas	922:933	90%) was relatively higher than that of others areas.					
31602930	6	63	from	umbellatus	960:969	arg1	Polysaccharides					936:950	Polysaccharides	936:950	Polysaccharides from P. umbellatus	936:969	Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose.					
30281305	1	0	theme	preharvest	153:162	arg1	application					164:174	preharvest application	153:174	preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	153:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	8	1	theme	elicitors	1088:1096	arg1	action					1072:1077	the action	1068:1077	the action of these elicitors	1068:1096	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	0	2	theme	Wine	68:71	arg1	Polysaccharide					73:86	Wine Polysaccharide	68:86	Wine Polysaccharide	68:86	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	5	3	from	content	795:801	arg1	wine					806:809	wine	806:809	wine	806:809	MeJ, BTH, CHSf, and particularly CHSs decrease the polysaccharide content in wine.					
30281305	1	4	theme	application	164:174	arg1	effect					143:148	the effect	139:148	the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions	139:387	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	0	5	theme	Oligosaccharide	92:106	arg1	Composition					108:118	Oligosaccharide Composition	92:118	Oligosaccharide Composition	92:118	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	1	6	from	fungi	288:292	arg1	jasmonate					233:241	[methyl jasmonate	225:241	[methyl jasmonate (MeJ)	225:247	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	MeJ					244:246	MeJ	244:246	MeJ	244:246	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	chitosan					274:281	chitosan	274:281	chitosan from fungi (CHSf)	274:299	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	elicitors					215:223	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan	200:313	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	200:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	chitosan					306:313	chitosan	306:313	chitosan	306:313	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	benzothiadiazole					250:265	benzothiadiazole	250:265	benzothiadiazole (BTH)	250:271	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	6	from	fungi	288:292	arg1	BTH					268:270	BTH	268:270	BTH	268:270	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	7	from	application	164:174	arg1	grapes					190:195	Monastrell grapes	179:195	Monastrell grapes	179:195	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	7	from	application	164:174	arg1	CHSs					329:332	CHSs	329:332	CHSs	329:332	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	7	from	application	164:174	arg1	seafood					320:326	seafood	320:326	seafood (CHSs)]	320:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	4	8	theme	Sugar	589:593	arg1	composition					595:605	Sugar composition	589:605	Sugar composition of oligosaccharides	589:625	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
30281305	8	9	theme	skin	1038:1041	arg1	wall					1048:1051	the skin cell wall	1034:1051	the skin cell wall	1034:1051	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	1	10	from	effect	143:148	arg1	grapes					190:195	Monastrell grapes	179:195	Monastrell grapes	179:195	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	10	from	effect	143:148	arg1	polysaccharide					344:357	wine polysaccharide and oligosaccharide fractions	339:387	polysaccharide	344:357	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	10	from	effect	143:148	arg1	fractions					379:387	wine polysaccharide and oligosaccharide fractions	339:387	fractions	379:387	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	4	11	theme	derivatives	716:726	arg1	GC					662:663	GC	662:663	GC of their per-O-trimethylsilylated methyl glycoside derivatives	662:726	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
30281305	9	12	from	application	1118:1128	arg1	clusters					1168:1175	the clusters	1164:1175	the clusters of the vineyard	1164:1191	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	8	13	theme	wall	1048:1051	arg1	cause					1000:1004	the cause	996:1004	the cause	996:1004	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	8	13	theme	wall	1048:1051	arg1	reinforcement					1017:1029	a reinforcement	1015:1029	a reinforcement of the skin cell wall	1015:1051	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	6	14	theme	CHS	874:876	arg1	treatments					878:887	both CHS treatments	869:887	both CHS treatments	869:887	The oligosaccharide concentration was also reduced after both CHS treatments.					
30281305	3	15	theme	polysaccharides	530:544	arg1	hydrolysis					516:525	hydrolysis	516:525	hydrolysis of polysaccharides	516:544	Neutral monosaccharides were released after hydrolysis of polysaccharides and quantified by gas chromatography (GC).					
30281305	9	16	theme	complex	1206:1212	arg1	composition					1227:1237	the complex carbohydrate composition	1202:1237	the complex carbohydrate composition of elaborated wine	1202:1256	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	1	17	theme	Monastrell	179:188	arg1	grapes					190:195	Monastrell grapes	179:195	Monastrell grapes	179:195	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	2	18	theme	oligosaccharide	413:427	arg1	fractions					429:437	The polysaccharide and oligosaccharide fractions	390:437	fractions	429:437	The polysaccharide and oligosaccharide fractions were isolated and characterized.					
30281305	5	19	theme	polysaccharide	780:793	arg1	content					795:801	the polysaccharide content	776:801	the polysaccharide content in wine	776:809	MeJ, BTH, CHSf, and particularly CHSs decrease the polysaccharide content in wine.					
30281305	4	20	theme	per-O-trimethylsilylated	674:697	arg1	derivatives					716:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
30281305	0	21	theme	Preharvest	0:9	arg1	Application					11:21	Preharvest Application	0:21	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.	0:119	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	1	22	from	seafood	320:326	arg1	jasmonate					233:241	[methyl jasmonate	225:241	[methyl jasmonate (MeJ)	225:247	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	22	from	seafood	320:326	arg1	application					164:174	preharvest application	153:174	preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	153:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	22	from	seafood	320:326	arg1	chitosan					274:281	chitosan	274:281	chitosan from fungi (CHSf)	274:299	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	22	from	seafood	320:326	arg1	elicitors					215:223	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan	200:313	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	200:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	22	from	seafood	320:326	arg1	chitosan					306:313	chitosan	306:313	chitosan	306:313	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	22	from	seafood	320:326	arg1	benzothiadiazole					250:265	benzothiadiazole	250:265	benzothiadiazole (BTH)	250:271	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	7	23	theme	skin	942:945	arg1	wall					952:955	the skin cell wall	938:955	the skin cell wall from treated grapes	938:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	7	24	from	degradation	923:933	arg1	grapes					970:975	treated grapes	962:975	treated grapes	962:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	0	25	theme	Elicitors	26:34	arg1	Application					11:21	Preharvest Application	0:21	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.	0:119	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	7	26	theme	treated	962:968	arg1	grapes					970:975	treated grapes	962:975	treated grapes	962:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	7	27	theme	cell	947:950	arg1	wall					952:955	the skin cell wall	938:955	the skin cell wall from treated grapes	938:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	9	28	theme	carbohydrate	1214:1225	arg1	composition					1227:1237	the complex carbohydrate composition	1202:1237	the complex carbohydrate composition of elaborated wine	1202:1256	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	3	29	theme	gas	564:566	arg1	GC					584:585	GC	584:585	GC	584:585	Neutral monosaccharides were released after hydrolysis of polysaccharides and quantified by gas chromatography (GC).					
30281305	3	29	theme	gas	564:566	arg1	chromatography					568:581	gas chromatography	564:581	gas chromatography (GC)	564:586	Neutral monosaccharides were released after hydrolysis of polysaccharides and quantified by gas chromatography (GC).					
30281305	1	30	theme	different	205:213	arg1	chitosan					306:313	chitosan	306:313	chitosan	306:313	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	30	theme	different	205:213	arg1	benzothiadiazole					250:265	benzothiadiazole	250:265	benzothiadiazole (BTH)	250:271	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	30	theme	different	205:213	arg1	jasmonate					233:241	[methyl jasmonate	225:241	[methyl jasmonate (MeJ)	225:247	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	30	theme	different	205:213	arg1	elicitors					215:223	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan	200:313	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	200:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	30	theme	different	205:213	arg1	chitosan					274:281	chitosan	274:281	chitosan from fungi (CHSf)	274:299	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	0	31	theme	Monastrell	39:48	arg1	Grapes					50:55	Monastrell Grapes	39:55	Monastrell Grapes	39:55	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	1	32	theme	elicitors	215:223	arg1	application					164:174	preharvest application	153:174	preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	153:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	8	33	theme	action	1072:1077	arg1	result					1058:1063	a result	1056:1063	a result of the action of these elicitors	1056:1096	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	1	34	theme	wine	339:342	arg1	polysaccharide					344:357	wine polysaccharide and oligosaccharide fractions	339:387	polysaccharide	344:357	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	2	35	theme	polysaccharide	394:407	arg1	fractions					429:437	The polysaccharide and oligosaccharide fractions	390:437	fractions	429:437	The polysaccharide and oligosaccharide fractions were isolated and characterized.					
30281305	1	36	theme	[methyl	225:231	arg1	MeJ					244:246	MeJ	244:246	MeJ	244:246	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	36	theme	[methyl	225:231	arg1	jasmonate					233:241	[methyl jasmonate	225:241	[methyl jasmonate (MeJ)	225:247	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	36	theme	[methyl	225:231	arg1	elicitors					215:223	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan	200:313	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	200:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	6	37	theme	oligosaccharide	816:830	arg1	concentration					832:844	The oligosaccharide concentration	812:844	The oligosaccharide concentration	812:844	The oligosaccharide concentration was also reduced after both CHS treatments.					
30281305	0	38	from	Impact	58:63	arg1	Polysaccharide					73:86	Wine Polysaccharide	68:86	Wine Polysaccharide	68:86	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	0	38	from	Impact	58:63	arg1	Composition					108:118	Oligosaccharide Composition	92:118	Oligosaccharide Composition	92:118	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	3	39	theme	Neutral	472:478	arg1	monosaccharides					480:494	Neutral monosaccharides	472:494	Neutral monosaccharides	472:494	Neutral monosaccharides were released after hydrolysis of polysaccharides and quantified by gas chromatography (GC).					
30281305	7	40	theme	wall	952:955	arg1	degradation					923:933	a lower degradation	915:933	a lower degradation of the skin cell wall from treated grapes	915:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	8	41	theme	cell	1043:1046	arg1	wall					1048:1051	the skin cell wall	1034:1051	the skin cell wall	1034:1051	We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors.					
30281305	9	42	theme	vineyard	1184:1191	arg1	clusters					1168:1175	the clusters	1164:1175	the clusters of the vineyard	1164:1191	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	1	43	theme	oligosaccharide	363:377	arg1	fractions					379:387	wine polysaccharide and oligosaccharide fractions	339:387	fractions	379:387	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	jasmonate					233:241	[methyl jasmonate	225:241	[methyl jasmonate (MeJ)	225:247	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	MeJ					244:246	MeJ	244:246	MeJ	244:246	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	chitosan					274:281	chitosan	274:281	chitosan from fungi (CHSf)	274:299	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	elicitors					215:223	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan	200:313	four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)]	200:334	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	chitosan					306:313	chitosan	306:313	chitosan	306:313	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	benzothiadiazole					250:265	benzothiadiazole	250:265	benzothiadiazole (BTH)	250:271	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	1	44	dep	elicitors	215:223	arg1	BTH					268:270	BTH	268:270	BTH	268:270	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	9	45	theme	elaborated	1242:1251	arg1	wine					1253:1256	elaborated wine	1242:1256	elaborated wine	1242:1256	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	1	46	from	grapes	190:195	arg1	effect					143:148	the effect	139:148	the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions	139:387	This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions.					
30281305	7	47	from	grapes	970:975	arg1	wall					952:955	the skin cell wall	938:955	the skin cell wall from treated grapes	938:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	7	47	from	grapes	970:975	arg1	degradation					923:933	a lower degradation	915:933	a lower degradation of the skin cell wall from treated grapes	915:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	7	48	theme	lower	917:921	arg1	degradation					923:933	a lower degradation	915:933	a lower degradation of the skin cell wall from treated grapes	915:975	These results pointed to a lower degradation of the skin cell wall from treated grapes.					
30281305	9	49	theme	wine	1253:1256	arg1	composition					1227:1237	the complex carbohydrate composition	1202:1237	the complex carbohydrate composition of elaborated wine	1202:1256	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	0	50	dep	Application	11:21	arg1	Impact					58:63	Impact	58:63	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.	0:119	Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition.					
30281305	9	51	theme	any	1133:1135	arg1	application					1118:1128	the application	1114:1128	the application of any of these four elicitors in the clusters of the vineyard	1114:1191	In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.					
30281305	4	52	theme	glycoside	706:714	arg1	derivatives					716:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
30281305	4	53	theme	methyl	699:704	arg1	derivatives					716:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	their per-O-trimethylsilylated methyl glycoside derivatives	668:726	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
30281305	4	54	theme	oligosaccharides	610:625	arg1	composition					595:605	Sugar composition	589:605	Sugar composition of oligosaccharides	589:625	Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives.					
31325025	0	0	theme	Enzymatic	91:99	arg1	Hydrolysis					101:110	Enzymatic Hydrolysis	91:110	Enzymatic Hydrolysis	91:110	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	6	1	theme	CBH	924:926	arg1	hydrolysis					899:908	The enzymatic hydrolysis	885:908	The enzymatic hydrolysis of HEEBPT and CBH	885:926	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	5	2	theme	%	804:804	arg1	content					791:797	xylan content	785:797	xylan content	785:797	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	8	3	theme	lignin	1237:1242	arg1	removal					1244:1250	lignin removal	1237:1250	lignin removal with NaClO2 from EB	1237:1270	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	3	4	theme	beneficial	521:530	arg1	bacteria					542:549	beneficial human gut bacteria	521:549	beneficial human gut bacteria	521:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	6	5	theme	significant	990:1000	arg1	difference					1002:1011	significant difference	990:1011	significant difference among the enzymatic extracts evaluated	990:1050	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	6	6	theme	HEEBPT	913:918	arg1	hydrolysis					899:908	The enzymatic hydrolysis	885:908	The enzymatic hydrolysis of HEEBPT and CBH	885:926	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	7	7	theme	feasible	1108:1115	arg1	alternative					1117:1127	a technically feasible alternative	1094:1127	a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry	1094:1225	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	7	7	theme	feasible	1108:1115	arg1	production					1061:1070	The XOS production	1053:1070	The XOS production from EB	1053:1078	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	7	8	from	EB	1077:1078	arg1	alternative					1117:1127	a technically feasible alternative	1094:1127	a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry	1094:1225	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	7	8	from	EB	1077:1078	arg1	production					1061:1070	The XOS production	1053:1070	The XOS production from EB	1053:1078	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	0	9	theme	Hydrolysis	101:110	arg1	Assessment					77:86	Assessment	77:86	Assessment of Enzymatic Hydrolysis	77:110	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	0	10	from	By-product	65:74	arg1	Characterization					15:30	Characterization	15:30	Characterization	15:30	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	0	10	from	By-product	65:74	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	5	11	located	found	834:838	arg1	CBH					879:881	CBH	879:881	CBH	879:881	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	11	located	found	834:838	arg1	hemicellulose					864:876	commercial Birchwood hemicellulose	843:876	commercial Birchwood hemicellulose (CBH)	843:882	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	11	located	found	834:838	arg2	%					832:832	58.5%	828:832	58.5% found in commercial Birchwood hemicellulose (CBH)	828:882	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	8	12	theme	process	1313:1319	arg1	feasibility					1284:1294	the feasibility	1280:1294	the feasibility of an industrial process because they generate toxic compounds in the pretreatment step	1280:1382	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	5	13	theme	commercial	843:852	arg1	CBH					879:881	CBH	879:881	CBH	879:881	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	13	theme	commercial	843:852	arg1	hemicellulose					864:876	commercial Birchwood hemicellulose	843:876	commercial Birchwood hemicellulose (CBH)	843:882	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	4	14	theme	hydrolysis	653:662	arg1	assessment					625:634	an assessment	622:634	an assessment of hemicellulose hydrolysis	622:662	This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS.					
31325025	3	15	theme	bacteria	542:549	arg1	stimulation					506:516	stimulation	506:516	stimulation	506:516	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	3	15	theme	bacteria	542:549	arg1	maintenance					490:500	maintenance	490:500	maintenance	490:500	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	1	16	theme	fibers	186:191	arg1	wood					156:159	Eucalyptus wood	145:159	Eucalyptus wood	145:159	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	1	16	theme	fibers	186:191	arg1	source					176:181	the primary source	164:181	the primary source of fibers to produce paper and cellulose in South American countries	164:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	5	17	theme	xylan	785:789	arg1	content					791:797	xylan content	785:797	xylan content	785:797	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	6	18	theme	enzymatic	889:897	arg1	hydrolysis					899:908	The enzymatic hydrolysis	885:908	The enzymatic hydrolysis of HEEBPT and CBH	885:926	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	9	19	from	EB	1511:1512	arg1	removal					1498:1504	lignin removal	1491:1504	lignin removal from EB	1491:1512	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	8	20	theme	pretreatment	1366:1377	arg1	step					1379:1382	the pretreatment step	1362:1382	the pretreatment step	1362:1382	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	3	21	theme	xylose-based	442:453	arg1	xylooligosaccharides					411:430	The xylooligosaccharides	407:430	The xylooligosaccharides (XOS)	407:436	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	3	21	theme	xylose-based	442:453	arg1	oligomers					455:463	xylose-based oligomers	442:463	xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria	442:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	4	22	theme	EB	576:577	arg1	extraction					579:588	the EB extraction	572:588	the EB extraction	572:588	This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS.					
31325025	10	23	dep	Abstract	1525:1532	arg1	Graphical					1515:1523	Graphical	1515:1523	Graphical	1515:1523	Graphical Abstract.					
31325025	2	24	theme	chip	334:337	arg1	by-product					390:399	Eucalyptus by-product	379:399	Eucalyptus by-product (EB)	379:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	24	theme	chip	334:337	arg1	production					344:353	chip wood production	334:353	chip wood production	334:353	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	7	25	theme	XOS	1057:1059	arg1	alternative					1117:1127	a technically feasible alternative	1094:1127	a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry	1094:1225	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	7	25	theme	XOS	1057:1059	arg1	production					1061:1070	The XOS production	1053:1070	The XOS production from EB	1053:1078	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	5	26	attach	derived	721:727	arg2	Hemicellulose					707:719	Hemicellulose	707:719	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT)	707:773	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	26	attach	derived	721:727	arg1	HEEBPT					767:772	HEEBPT	767:772	HEEBPT	767:772	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	26	attach	derived	721:727	arg1	extracted					734:742	extracted	734:742	extracted	734:742	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	26	attach	derived	721:727	arg1	pretreated					755:764	pretreated	755:764	pretreated	755:764	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	7	27	theme	hemicellulosic	1167:1180	arg1	fraction					1182:1189	hemicellulosic fraction	1167:1189	hemicellulosic fraction generated in the cellulose industry	1167:1225	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	6	28	theme	xylan	967:971	arg1	conversion					953:962	maximum conversion	945:962	maximum conversion of xylan into XOS	945:980	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	3	29	with	oligomers	455:463	arg1	effects					477:483	proven effects	470:483	proven effects over maintenance and stimulation of beneficial human gut bacteria	470:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	3	30	theme	proven	470:475	arg1	effects					477:483	proven effects	470:483	proven effects over maintenance and stimulation of beneficial human gut bacteria	470:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	2	31	theme	cellulose	291:299	arg1	industry					301:308	the cellulose industry	287:308	the cellulose industry	287:308	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	8	32	theme	toxic	1343:1347	arg1	compounds					1349:1357	toxic compounds	1343:1357	toxic compounds	1343:1357	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	9	33	theme	ionic	1442:1446	arg1	liquids					1448:1454	ionic liquids	1442:1454	ionic liquids	1442:1454	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	3	34	theme	human	532:536	arg1	bacteria					542:549	beneficial human gut bacteria	521:549	beneficial human gut bacteria	521:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
31325025	2	35	attach	derived	321:327	arg2	by-product					263:272	The major by-product	253:272	The major by-product generated in the cellulose industry	253:308	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	35	attach	derived	321:327	arg1	by-product					390:399	Eucalyptus by-product	379:399	Eucalyptus by-product (EB)	379:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	35	attach	derived	321:327	arg2	sawdust					313:319	sawdust	313:319	sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB)	313:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	35	attach	derived	321:327	arg1	production					344:353	chip wood production	334:353	chip wood production	334:353	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	5	36	theme	Birchwood	854:862	arg1	CBH					879:881	CBH	879:881	CBH	879:881	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	5	36	theme	Birchwood	854:862	arg1	hemicellulose					864:876	commercial Birchwood hemicellulose	843:876	commercial Birchwood hemicellulose (CBH)	843:882	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	0	37	theme	Hemicellulose	35:47	arg1	Characterization					15:30	Characterization	15:30	Characterization	15:30	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	0	37	theme	Hemicellulose	35:47	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	6	38	theme	maximum	945:951	arg1	conversion					953:962	maximum conversion	945:962	maximum conversion of xylan into XOS	945:980	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	9	39	theme	alternative	1412:1422	arg1	liquids					1448:1454	ionic liquids	1442:1454	ionic liquids	1442:1454	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	9	39	theme	alternative	1412:1422	arg1	reagents					1424:1431	alternative reagents	1412:1431	alternative reagents	1412:1431	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	8	40	from	EB	1269:1270	arg1	removal					1244:1250	lignin removal	1237:1250	lignin removal with NaClO2 from EB	1237:1270	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	4	41	theme	commercial	670:679	arg1	xylanases					681:689	commercial xylanases	670:689	commercial xylanases	670:689	This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS.					
31325025	8	42	theme	industrial	1302:1311	arg1	process					1313:1319	an industrial process	1299:1319	an industrial process because they generate toxic compounds in the pretreatment step	1299:1382	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	0	43	theme	Eucalyptus	54:63	arg1	By-product					65:74	Eucalyptus By-product	54:74	Eucalyptus By-product	54:74	Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.					
31325025	7	44	theme	cellulose	1208:1216	arg1	industry					1218:1225	the cellulose industry	1204:1225	the cellulose industry	1204:1225	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	1	45	theme	primary	168:174	arg1	wood					156:159	Eucalyptus wood	145:159	Eucalyptus wood	145:159	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	1	45	theme	primary	168:174	arg1	source					176:181	the primary source	164:181	the primary source of fibers to produce paper and cellulose in South American countries	164:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	1	46	theme	Eucalyptus	145:154	arg1	wood					156:159	Eucalyptus wood	145:159	Eucalyptus wood	145:159	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	1	46	theme	Eucalyptus	145:154	arg1	source					176:181	the primary source	164:181	the primary source of fibers to produce paper and cellulose in South American countries	164:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	9	47	theme	further	1391:1397	arg1	studies					1399:1405	further studies	1391:1405	further studies with alternative reagents, such as ionic liquids,	1391:1455	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	7	48	theme	value-added	1142:1152	arg1	product					1154:1160	a value-added product	1140:1160	a value-added product	1140:1160	The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry.					
31325025	1	49	from	cellulose	214:222	arg1	countries					242:250	South American countries	227:250	South American countries	227:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	1	50	theme	American	233:240	arg1	countries					242:250	South American countries	227:250	South American countries	227:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	8	51	dep	process	1313:1319	arg1	generate					1334:1341	generate	1334:1341	generate toxic compounds in the pretreatment step	1334:1382	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	8	52	with	removal	1244:1250	arg1	NaClO2					1257:1262	NaClO2	1257:1262	NaClO2	1257:1262	However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step.					
31325025	2	53	theme	wood	339:342	arg1	by-product					390:399	Eucalyptus by-product	379:399	Eucalyptus by-product (EB)	379:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	53	theme	wood	339:342	arg1	production					344:353	chip wood production	334:353	chip wood production	334:353	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	6	54	theme	enzymatic	1023:1031	arg1	extracts					1033:1040	the enzymatic extracts	1019:1040	the enzymatic extracts evaluated	1019:1050	The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated.					
31325025	5	55	dep	pretreated	755:764	arg1	NaClO2					748:753	NaClO2	748:753	NaClO2	748:753	Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH).					
31325025	9	56	theme	lignin	1491:1496	arg1	removal					1498:1504	lignin removal	1491:1504	lignin removal from EB	1491:1512	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	1	57	from	paper	204:208	arg1	countries					242:250	South American countries	227:250	South American countries	227:250	Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries.					
31325025	2	58	theme	Eucalyptus	379:388	arg1	EB					402:403	EB	402:403	EB	402:403	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	58	theme	Eucalyptus	379:388	arg1	by-product					390:399	Eucalyptus by-product	379:399	Eucalyptus by-product (EB)	379:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	58	theme	Eucalyptus	379:388	arg1	production					344:353	chip wood production	334:353	chip wood production	334:353	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	4	59	theme	hemicellulose	639:651	arg1	hydrolysis					653:662	hemicellulose hydrolysis	639:662	hemicellulose hydrolysis	639:662	This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS.					
31325025	2	60	theme	major	257:261	arg1	by-product					263:272	The major by-product	253:272	The major by-product generated in the cellulose industry	253:308	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	2	60	theme	major	257:261	arg1	sawdust					313:319	sawdust	313:319	sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB)	313:404	The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB).					
31325025	9	61	with	studies	1399:1405	arg1	liquids					1448:1454	ionic liquids	1442:1454	ionic liquids	1442:1454	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	9	61	with	studies	1399:1405	arg1	reagents					1424:1431	alternative reagents	1412:1431	alternative reagents	1412:1431	Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB.					
31325025	3	62	theme	gut	538:540	arg1	bacteria					542:549	beneficial human gut bacteria	521:549	beneficial human gut bacteria	521:549	The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria.					
29441417	11	0	from	processing	1716:1725	arg1	IVDs					1738:1741	the AIS IVDs	1730:1741	the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1730:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	3	1	theme	Sepharose	505:513	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	1	theme	Sepharose	505:513	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	4	2	from	IVDs	686:689	arg1	content					646:652	The KS and CS content	632:652	content	646:652	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	4	2	from	IVDs	686:689	arg1	extracts					668:675	IVD tissue extracts	657:675	IVD tissue extracts from AIS IVDs	657:689	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	6	3	theme	IVD	903:905	arg1	populations					916:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	3	4	theme	polyacrylamide	554:567	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	4	theme	polyacrylamide	554:567	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	4	theme	polyacrylamide	554:567	arg1	CAPAGE					590:595	CAPAGE	590:595	CAPAGE	590:595	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	6	5	theme	idiopathic	882:891	arg1	populations					916:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	11	6	from	IVDs	1692:1695	arg1	polydispersity					1608:1621	The polydispersity	1604:1621	The polydispersity	1604:1621	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	11	6	from	IVDs	1692:1695	arg1	similar					1658:1664	similar	1658:1664	similar	1658:1664	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	4	7	theme	tissue	661:666	arg1	extracts					668:675	IVD tissue extracts	657:675	IVD tissue extracts from AIS IVDs	657:689	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	3	8	theme	composite	536:544	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	8	theme	composite	536:544	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	8	theme	composite	536:544	arg1	CAPAGE					590:595	CAPAGE	590:595	CAPAGE	590:595	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	5	9	theme	similar	847:853	arg1	manner					855:860	a similar manner	845:860	a similar manner	845:860	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	8	10	theme	human	1206:1210	arg1	IVDs					1222:1225	aged human and ovine IVDs	1201:1225	aged human and ovine IVDs	1201:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	6	11	with	levels	943:948	arg1	hyaluronan					993:1002	hyaluronan	993:1002	hyaluronan	993:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	11	12	from	similar	1658:1664	arg1	IVDs					1692:1695	old human and ovine IVDs	1672:1695	old human and ovine IVDs	1672:1695	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	9	13	theme	Sulphated	1228:1236	arg1	5-D-4					1242:1246	5-D-4	1242:1246	5-D-4	1242:1246	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	13	theme	Sulphated	1228:1236	arg1	KS					1238:1239	Sulphated KS	1228:1239	Sulphated KS (5-D-4)	1228:1247	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	8	14	contain	had	1169:1171	arg1	IVDs					1164:1167	AIS IVDs	1160:1167	AIS IVDs	1160:1167	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	8	14	contain	had	1169:1171	arg2	compositions					1177:1188	GAG compositions	1173:1188	GAG compositions similar to aged human and ovine IVDs	1173:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	8	15	theme	ovine	1216:1220	arg1	IVDs					1222:1225	aged human and ovine IVDs	1201:1225	aged human and ovine IVDs	1201:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	4	16	theme	CS	643:644	arg1	content					646:652	The KS and CS content	632:652	content	646:652	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	0	17	theme	aged	128:131	arg1	IVDs					149:152	aged human and ovine IVDs	128:152	aged human and ovine IVDs	128:152	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	1	18	theme	aged	382:385	arg1	IVDs					393:396	age matched and aged human IVDs	366:396	age matched and aged human IVDs	366:396	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	11	19	theme	biomechanical	1762:1774	arg1	microenvironments					1776:1792	the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1758:1835	the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1758:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	10	20	theme	CS	1552:1553	arg1	isomer					1555:1560	the 2-B-6(+) CS isomer	1539:1560	the 2-B-6(+) CS isomer	1539:1560	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	7	21	theme	aged	1085:1088	arg1	IVDs					1106:1109	aged human and ovine IVDs	1085:1109	aged human and ovine IVDs	1085:1109	CAPAGE demonstrated three aggrecan populations in AIS, aged human and ovine IVDs increased polydispersity and mobility in CAPAGE.					
29441417	5	22	theme	ovine	812:816	arg1	IVDs					818:821	10-year-old ovine IVDs	800:821	10-year-old ovine IVDs	800:821	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	1	23	theme	altered	211:217	arg1	biomechanics					219:230	altered biomechanics	211:230	altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	211:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	24	from	biomechanics	219:230	arg1	IVDs					295:298	IVDs	295:298	IVDs	295:298	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	24	from	biomechanics	219:230	arg1	AIS					268:270	AIS	268:270	AIS	268:270	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	24	from	biomechanics	219:230	arg1	discs					288:292	adolescent idiopathic scoliotic (AIS) intervertebral discs	235:292	adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	235:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	4	25	theme	age-matched	710:720	arg1	IVDs					740:743	age-matched normal adolescent IVDs	710:743	age-matched normal adolescent IVDs	710:743	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	6	26	theme	similar	935:941	arg1	proteoglycans					1015:1027	old IVD proteoglycans	1007:1027	old IVD proteoglycans	1007:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	6	26	theme	similar	935:941	arg1	levels					943:948	similar levels	935:948	similar levels of polydispersity and aggregatability with hyaluronan	935:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	4	27	theme	adolescent	729:738	arg1	IVDs					740:743	age-matched normal adolescent IVDs	710:743	age-matched normal adolescent IVDs	710:743	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	12	28	theme	Supplementary	1885:1897	arg1	Material					1899:1906	Electronic Supplementary Material	1874:1906	Electronic Supplementary Material	1874:1906	These slides can be retrieved under Electronic Supplementary Material.					
29441417	0	29	theme	glycosaminoglycan	79:95	arg1	composition					97:107	a glycosaminoglycan composition	77:107	a glycosaminoglycan composition	77:107	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	9	30	theme	were	1286:1289	arg1	markers					1291:1297	3-B-3(+) were markers	1277:1297	3-B-3(+) were markers of tissue maturation	1277:1318	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	6	31	theme	IVD	1011:1013	arg1	proteoglycans					1015:1027	old IVD proteoglycans	1007:1027	old IVD proteoglycans	1007:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	6	31	theme	IVD	1011:1013	arg1	levels					943:948	similar levels	935:948	similar levels of polydispersity and aggregatability with hyaluronan	935:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	11	32	from	polydispersity	1608:1621	arg1	IVDs					1642:1645	AIS IVDs	1638:1645	AIS IVDs	1638:1645	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	11	33	theme	old	1672:1674	arg1	IVDs					1692:1695	old human and ovine IVDs	1672:1695	old human and ovine IVDs	1672:1695	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	0	34	theme	similar	109:115	arg1	aggrecanolysis					58:71	advanced aggrecanolysis	49:71	advanced aggrecanolysis	49:71	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	10	35	theme	immature	1582:1589	arg1	tissues					1595:1601	immature IVD tissues	1582:1601	immature IVD tissues	1582:1601	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	1	36	theme	matched	370:376	arg1	IVDs					393:396	age matched and aged human IVDs	366:396	age matched and aged human IVDs	366:396	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	37	dep	PURPOSE	155:161	arg1	designed					185:192	designed	185:192	was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs	181:396	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	11	38	theme	disc	1798:1801	arg1	cells					1803:1807	the disc cells	1794:1807	the disc cells	1794:1807	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	5	39	theme	10-year-old	800:810	arg1	IVDs					818:821	10-year-old ovine IVDs	800:821	10-year-old ovine IVDs	800:821	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	1	40	theme	intervertebral	273:286	arg1	IVDs					295:298	IVDs	295:298	IVDs	295:298	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	40	theme	intervertebral	273:286	arg1	AIS					268:270	AIS	268:270	AIS	268:270	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	40	theme	intervertebral	273:286	arg1	discs					288:292	adolescent idiopathic scoliotic (AIS) intervertebral discs	235:292	adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	235:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	2	41	theme	ovine	427:431	arg1	IVDs					433:436	Newborn, 2- and 10-year-old ovine IVDs	399:436	IVDs	433:436	Newborn, 2- and 10-year-old ovine IVDs were also examined.					
29441417	4	42	theme	old	754:756	arg1	IVDs					764:767	old human IVDs	754:767	old human IVDs	754:767	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	11	43	theme	AIS	1828:1830	arg1	IVDs					1832:1835	AIS IVDs	1828:1835	AIS IVDs	1828:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	0	44	theme	idiopathic	15:24	arg1	IVD					36:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	10	45	theme	3-B-3	1459:1463	arg1	CS					1468:1469	3-B-3(+) CS	1459:1469	3-B-3(+) CS	1459:1469	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	0	46	theme	advanced	49:56	arg1	aggrecanolysis					58:71	advanced aggrecanolysis	49:71	advanced aggrecanolysis	49:71	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	1	47	theme	tissue	310:315	arg1	compositions					317:328	tissue compositions	310:328	tissue compositions	310:328	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	9	48	from	IVDs	1384:1387	arg1	prominent					1362:1370	prominent	1362:1370	prominent	1362:1370	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	3	49	theme	Aggrecan	466:473	arg1	populations					475:485	METHODS Aggrecan populations	458:485	METHODS Aggrecan populations	458:485	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	6	50	dep	RESULTS	863:869	arg1	shared					928:933	shared	928:933	shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans	928:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	10	51	theme	maturation	1508:1517	arg1	IVDs					1527:1530	IVD maturation and AIS IVDs	1504:1530	IVD maturation and AIS IVDs	1504:1530	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	2	52	theme	Newborn	399:405	arg1	IVDs					433:436	Newborn, 2- and 10-year-old ovine IVDs	399:436	IVDs	433:436	Newborn, 2- and 10-year-old ovine IVDs were also examined.					
29441417	3	53	theme	CL2B	515:518	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	53	theme	CL2B	515:518	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	9	54	theme	immature	1375:1382	arg1	IVDs					1384:1387	immature IVDs	1375:1387	immature IVDs	1375:1387	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	1	55	theme	scoliotic	257:265	arg1	IVDs					295:298	IVDs	295:298	IVDs	295:298	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	55	theme	scoliotic	257:265	arg1	AIS					268:270	AIS	268:270	AIS	268:270	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	55	theme	scoliotic	257:265	arg1	discs					288:292	adolescent idiopathic scoliotic (AIS) intervertebral discs	235:292	adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	235:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	6	56	theme	Aggrecan	907:914	arg1	populations					916:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	3	57	theme	agarose	546:552	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	57	theme	agarose	546:552	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	57	theme	agarose	546:552	arg1	CAPAGE					590:595	CAPAGE	590:595	CAPAGE	590:595	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	8	58	theme	GAG	1173:1175	arg1	compositions					1177:1188	GAG compositions	1173:1188	GAG compositions similar to aged human and ovine IVDs	1173:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	6	59	theme	scoliotic	893:901	arg1	populations					916:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	10	60	theme	AIS	1523:1525	arg1	IVDs					1527:1530	IVD maturation and AIS IVDs	1504:1530	IVD maturation and AIS IVDs	1504:1530	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	1	61	theme	present	167:173	arg1	study					175:179	The present study	163:179	The present study	163:179	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	8	62	theme	similar	1190:1196	arg1	compositions					1177:1188	GAG compositions	1173:1188	GAG compositions similar to aged human and ovine IVDs	1173:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	6	63	theme	Adolescent	871:880	arg1	populations					916:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	Adolescent idiopathic scoliotic IVD Aggrecan populations	871:926	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	4	64	theme	IVD	657:659	arg1	extracts					668:675	IVD tissue extracts	657:675	IVD tissue extracts from AIS IVDs	657:689	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	7	65	theme	ovine	1100:1104	arg1	IVDs					1106:1109	aged human and ovine IVDs	1085:1109	aged human and ovine IVDs	1085:1109	CAPAGE demonstrated three aggrecan populations in AIS, aged human and ovine IVDs increased polydispersity and mobility in CAPAGE.					
29441417	9	66	theme	mature	1418:1423	arg1	IVDs					1425:1428	mature IVDs	1418:1428	mature IVDs	1418:1428	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	8	67	theme	aged	1201:1204	arg1	IVDs					1222:1225	aged human and ovine IVDs	1201:1225	aged human and ovine IVDs	1201:1225	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	4	68	theme	extracts	668:675	arg1	content					646:652	The KS and CS content	632:652	content	646:652	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	11	69	theme	altered	1708:1714	arg1	processing					1716:1725	altered processing	1708:1725	altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1708:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	0	70	theme	human	133:137	arg1	IVDs					149:152	aged human and ovine IVDs	128:152	aged human and ovine IVDs	128:152	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	3	71	theme	gel	569:571	arg1	electrophoresis					573:587	composite agarose polyacrylamide gel electrophoresis	536:587	composite agarose polyacrylamide gel electrophoresis (CAPAGE)	536:596	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	71	theme	gel	569:571	arg1	chromatography					520:533	Sepharose CL2B chromatography	505:533	Sepharose CL2B chromatography	505:533	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	3	71	theme	gel	569:571	arg1	CAPAGE					590:595	CAPAGE	590:595	CAPAGE	590:595	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	4	72	from	content	646:652	arg1	IVDs					686:689	AIS IVDs	682:689	AIS IVDs	682:689	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	6	73	theme	aggregatability	972:986	arg1	proteoglycans					1015:1027	old IVD proteoglycans	1007:1027	old IVD proteoglycans	1007:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	6	73	theme	aggregatability	972:986	arg1	levels					943:948	similar levels	935:948	similar levels of polydispersity and aggregatability with hyaluronan	935:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	0	74	theme	ovine	143:147	arg1	IVDs					149:152	aged human and ovine IVDs	128:152	aged human and ovine IVDs	128:152	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	1	75	theme	human	387:391	arg1	IVDs					393:396	age matched and aged human IVDs	366:396	age matched and aged human IVDs	366:396	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	6	76	theme	polydispersity	953:966	arg1	proteoglycans					1015:1027	old IVD proteoglycans	1007:1027	old IVD proteoglycans	1007:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	6	76	theme	polydispersity	953:966	arg1	levels					943:948	similar levels	935:948	similar levels of polydispersity and aggregatability with hyaluronan	935:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	12	77	theme	Electronic	1874:1883	arg1	Material					1899:1906	Electronic Supplementary Material	1874:1906	Electronic Supplementary Material	1874:1906	These slides can be retrieved under Electronic Supplementary Material.					
29441417	11	78	theme	AIS	1734:1736	arg1	IVDs					1738:1741	the AIS IVDs	1730:1741	the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1730:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	7	79	theme	human	1090:1094	arg1	IVDs					1106:1109	aged human and ovine IVDs	1085:1109	aged human and ovine IVDs	1085:1109	CAPAGE demonstrated three aggrecan populations in AIS, aged human and ovine IVDs increased polydispersity and mobility in CAPAGE.					
29441417	7	80	theme	aggrecan	1056:1063	arg1	populations					1065:1075	three aggrecan populations	1050:1075	three aggrecan populations in AIS	1050:1082	CAPAGE demonstrated three aggrecan populations in AIS, aged human and ovine IVDs increased polydispersity and mobility in CAPAGE.					
29441417	4	81	theme	normal	722:727	arg1	IVDs					740:743	age-matched normal adolescent IVDs	710:743	age-matched normal adolescent IVDs	710:743	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	4	82	theme	AIS	682:684	arg1	IVDs					686:689	AIS IVDs	682:689	AIS IVDs	682:689	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	5	83	from	2-	793:794	arg1	Extracts					770:777	Extracts	770:777	Extracts from newborn, 2- and 10-year-old ovine IVDs	770:821	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	11	84	theme	ovine	1686:1690	arg1	IVDs					1692:1695	old human and ovine IVDs	1672:1695	old human and ovine IVDs	1672:1695	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	5	85	from	newborn	784:790	arg1	Extracts					770:777	Extracts	770:777	Extracts from newborn, 2- and 10-year-old ovine IVDs	770:821	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	6	86	theme	old	1007:1009	arg1	proteoglycans					1015:1027	old IVD proteoglycans	1007:1027	old IVD proteoglycans	1007:1027	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	6	86	theme	old	1007:1009	arg1	levels					943:948	similar levels	935:948	similar levels of polydispersity and aggregatability with hyaluronan	935:1002	RESULTS Adolescent idiopathic scoliotic IVD Aggrecan populations shared similar levels of polydispersity and aggregatability with hyaluronan as old IVD proteoglycans.					
29441417	4	87	theme	human	758:762	arg1	IVDs					764:767	old human IVDs	754:767	old human IVDs	754:767	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	11	88	theme	human	1676:1680	arg1	IVDs					1692:1695	old human and ovine IVDs	1672:1695	old human and ovine IVDs	1672:1695	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	5	89	from	IVDs	818:821	arg1	Extracts					770:777	Extracts	770:777	Extracts from newborn, 2- and 10-year-old ovine IVDs	770:821	Extracts from newborn, 2- and 10-year-old ovine IVDs were also examined in a similar manner.					
29441417	10	90	theme	Sulphated	1442:1450	arg1	KS					1452:1453	DISCUSSION Sulphated KS	1431:1453	DISCUSSION Sulphated KS	1431:1453	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	11	91	from	IVDs	1738:1741	arg1	response					1746:1753	response	1746:1753	response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs	1746:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	7	92	from	populations	1065:1075	arg1	AIS					1080:1082	AIS	1080:1082	AIS	1080:1082	CAPAGE demonstrated three aggrecan populations in AIS, aged human and ovine IVDs increased polydispersity and mobility in CAPAGE.					
29441417	0	93	theme	adolescent	4:13	arg1	IVD					36:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	9	94	theme	tissue	1302:1307	arg1	maturation					1309:1318	tissue maturation	1302:1318	tissue maturation	1302:1318	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	95	dep	were	1286:1289	arg1	3-B-3					1277:1281	3-B-3	1277:1281	3-B-3	1277:1281	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	0	96	theme	scoliotic	26:34	arg1	IVD					36:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD	0:38	The adolescent idiopathic scoliotic IVD displays advanced aggrecanolysis and a glycosaminoglycan composition similar to that of aged human and ovine IVDs.					
29441417	11	97	dep	microenvironments	1776:1792	arg1	exposed					1814:1820	exposed	1814:1820	were exposed to in AIS IVDs	1809:1835	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	2	98	theme	10-year-old	415:425	arg1	IVDs					433:436	Newborn, 2- and 10-year-old ovine IVDs	399:436	IVDs	433:436	Newborn, 2- and 10-year-old ovine IVDs were also examined.					
29441417	11	99	theme	aggrecan	1626:1633	arg1	polydispersity					1608:1621	The polydispersity	1604:1621	The polydispersity	1604:1621	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	11	99	theme	aggrecan	1626:1633	arg1	similar					1658:1664	similar	1658:1664	similar	1658:1664	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	3	100	theme	METHODS	458:464	arg1	populations					475:485	METHODS Aggrecan populations	458:485	METHODS Aggrecan populations	458:485	METHODS Aggrecan populations were separated by Sepharose CL2B chromatography, composite agarose polyacrylamide gel electrophoresis (CAPAGE) and identified by immunoblotting.					
29441417	1	101	theme	adolescent	235:244	arg1	IVDs					295:298	IVDs	295:298	IVDs	295:298	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	101	theme	adolescent	235:244	arg1	AIS					268:270	AIS	268:270	AIS	268:270	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	101	theme	adolescent	235:244	arg1	discs					288:292	adolescent idiopathic scoliotic (AIS) intervertebral discs	235:292	adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	235:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	10	102	theme	IVD	1591:1593	arg1	tissues					1595:1601	immature IVD tissues	1582:1601	immature IVD tissues	1582:1601	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	9	103	theme	maturation	1309:1318	arg1	5-D-4					1242:1246	5-D-4	1242:1246	5-D-4	1242:1246	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	103	theme	maturation	1309:1318	arg1	markers					1291:1297	3-B-3(+) were markers	1277:1297	3-B-3(+) were markers of tissue maturation	1277:1318	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	103	theme	maturation	1309:1318	arg1	chondroitin-4-sulphate					1325:1346	chondroitin-4-sulphate	1325:1346	chondroitin-4-sulphate	1325:1346	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	103	theme	maturation	1309:1318	arg1	chondroitin-6-sulphate					1253:1274	chondroitin-6-sulphate	1253:1274	chondroitin-6-sulphate	1253:1274	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	103	theme	maturation	1309:1318	arg1	KS					1238:1239	Sulphated KS	1228:1239	Sulphated KS (5-D-4)	1228:1247	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	103	theme	maturation	1309:1318	arg1	2-B-6					1349:1353	2-B-6	1349:1353	2-B-6	1349:1353	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	9	104	from	prominent	1362:1370	arg1	IVDs					1384:1387	immature IVDs	1375:1387	immature IVDs	1375:1387	Sulphated KS (5-D-4) and chondroitin-6-sulphate, 3-B-3(+) were markers of tissue maturation, and chondroitin-4-sulphate, 2-B-6(+) was prominent in immature IVDs but its levels were lower in mature IVDs.					
29441417	11	105	theme	AIS	1638:1640	arg1	IVDs					1642:1645	AIS IVDs	1638:1645	AIS IVDs	1638:1645	The polydispersity of aggrecan in AIS IVDs, which was similar to in old human and ovine IVDs, reflected altered processing in the AIS IVDs in response to the biomechanical microenvironments the disc cells were exposed to in AIS IVDs.					
29441417	8	106	theme	AIS	1160:1162	arg1	IVDs					1164:1167	AIS IVDs	1160:1167	AIS IVDs	1160:1167	AIS IVDs had GAG compositions similar to aged human and ovine IVDs.					
29441417	4	107	theme	KS	636:637	arg1	content					646:652	The KS and CS content	632:652	content	646:652	The KS and CS content of IVD tissue extracts from AIS IVDs were compared with age-matched normal adolescent IVDs and with old human IVDs.					
29441417	10	108	theme	IVD	1504:1506	arg1	maturation					1508:1517	IVD maturation	1504:1517	IVD maturation	1504:1517	DISCUSSION Sulphated KS and 3-B-3(+) CS were prominently associated with IVD maturation and AIS IVDs, while the 2-B-6(+) CS isomer was associated with immature IVD tissues.					
29441417	1	109	theme	idiopathic	246:255	arg1	IVDs					295:298	IVDs	295:298	IVDs	295:298	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	109	theme	idiopathic	246:255	arg1	AIS					268:270	AIS	268:270	AIS	268:270	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
29441417	1	109	theme	idiopathic	246:255	arg1	discs					288:292	adolescent idiopathic scoliotic (AIS) intervertebral discs	235:292	adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs)	235:299	PURPOSE The present study was designed to ascertain how altered biomechanics in adolescent idiopathic scoliotic (AIS) intervertebral discs (IVDs) affected tissue compositions and aggrecan processing compared to age matched and aged human IVDs.					
30342144	0	0	theme	surface	101:107	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	7	1	theme	typical	1212:1218	arg1	characteristics					1196:1210	characteristics	1196:1210	characteristics typical of amorphous powders	1196:1239	The microstructure of CYP exhibited characteristics typical of amorphous powders.					
30342144	2	2	theme	surface	377:383	arg1	RSM					398:400	RSM	398:400	RSM	398:400	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	2	theme	surface	377:383	arg1	methodology					385:395	response surface methodology	368:395	response surface methodology (RSM)	368:401	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	2	theme	surface	377:383	arg1	experiments					326:336	single-factor experiments	312:336	single-factor experiments	312:336	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	5	3	with	purification	823:834	arg1	exchange					847:854	anion exchange	841:854	anion exchange (DE-52)	841:862	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	3	with	purification	823:834	arg1	DE-52					857:861	DE-52	857:861	DE-52	857:861	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	3	with	purification	823:834	arg1	columns					900:906	size-exclusion (Sephadex G-100) columns	868:906	size-exclusion (Sephadex G-100) columns	868:906	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	9	4	theme	inhibitory	1421:1430	arg1	effect					1432:1437	a relatively strong inhibitory effect	1401:1437	a relatively strong inhibitory effect on BGC-823 cell growth	1401:1460	Moreover, CYP exhibited a relatively strong inhibitory effect on BGC-823 cell growth.					
30342144	0	5	theme	response	92:99	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	2	6	theme	response	368:375	arg1	RSM					398:400	RSM	398:400	RSM	398:400	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	6	theme	response	368:375	arg1	methodology					385:395	response surface methodology	368:395	response surface methodology (RSM)	368:401	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	6	theme	response	368:375	arg1	experiments					326:336	single-factor experiments	312:336	single-factor experiments	312:336	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	5	7	theme	50.8	968:971	arg1	%					972:972	%	972:972	%	972:972	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	2	8	theme	polysaccharide	534:547	arg1	rate					514:517	the extraction rate	499:517	the extraction rate of Chinese yam polysaccharide (CYP)	499:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	6	9	theme	polysaccharides	1143:1157	arg1	peaks					1130:1134	the characteristic absorption peaks	1100:1134	the characteristic absorption peaks of the polysaccharides	1100:1157	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	2	10	theme	single-factor	312:324	arg1	methodology					385:395	response surface methodology	368:395	response surface methodology (RSM)	368:401	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	10	theme	single-factor	312:324	arg1	experiments					326:336	single-factor experiments	312:336	single-factor experiments	312:336	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	10	theme	single-factor	312:324	arg1	design					351:356	Box-Behnken design	339:356	Box-Behnken design (BBD)	339:362	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	6	11	theme	absorption	1119:1128	arg1	peaks					1130:1134	the characteristic absorption peaks	1100:1134	the characteristic absorption peaks of the polysaccharides	1100:1157	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	2	12	theme	yam	530:532	arg1	CYP					550:552	CYP	550:552	CYP	550:552	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	12	theme	yam	530:532	arg1	polysaccharide					534:547	Chinese yam polysaccharide	522:547	Chinese yam polysaccharide (CYP)	522:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	6	13	theme	spectroscopy	1053:1064	arg1	characterization					1066:1081	infrared (FT-IR) spectroscopy characterization	1036:1081	infrared (FT-IR) spectroscopy characterization of CYP	1036:1088	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	6	14	theme	CYP	1086:1088	arg1	characterization					1066:1081	infrared (FT-IR) spectroscopy characterization	1036:1081	infrared (FT-IR) spectroscopy characterization of CYP	1036:1088	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	0	15	theme	yam	67:69	arg1	polysaccharide					71:84	Chinese yam polysaccharide	59:84	Chinese yam polysaccharide	59:84	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	4	16	theme	optimal	752:758	arg1	conditions					760:769	these optimal conditions	746:769	these optimal conditions	746:769	Under these optimal conditions, a CYP extraction rate of 88.7% was achieved.					
30342144	6	17	dep	Fourier	1018:1024	arg1	transform					1026:1034	transform	1026:1034	transform infrared (FT-IR) spectroscopy characterization of CYP	1026:1088	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	6	18	theme	infrared	1036:1043	arg1	spectroscopy					1053:1064	infrared (FT-IR) spectroscopy	1036:1064	infrared (FT-IR) spectroscopy characterization of CYP	1036:1088	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	6	19	theme	FT-IR	1046:1050	arg1	spectroscopy					1053:1064	infrared (FT-IR) spectroscopy	1036:1064	infrared (FT-IR) spectroscopy characterization of CYP	1036:1088	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	7	20	theme	CYP	1182:1184	arg1	microstructure					1164:1177	The microstructure	1160:1177	The microstructure of CYP	1160:1184	The microstructure of CYP exhibited characteristics typical of amorphous powders.					
30342144	8	21	theme	anion	1361:1365	arg1	radicals					1367:1374	superoxide anion radicals	1350:1374	superoxide anion radicals	1350:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	2	22	theme	raw	298:300	arg1	yam					260:262	Baiyu yam	254:262	Baiyu yam (Dioscorea opposita Thunb)	254:289	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	22	theme	raw	298:300	arg1	material					302:309	the raw material	294:309	the raw material	294:309	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	0	23	theme	biological	129:138	arg1	activity					140:147	its biological activity	125:147	its biological activity	125:147	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	2	24	theme	pH	467:468	arg1	effects					436:442	the effects	432:442	the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP)	432:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	7	25	theme	amorphous	1223:1231	arg1	powders					1233:1239	amorphous powders	1223:1239	amorphous powders	1223:1239	The microstructure of CYP exhibited characteristics typical of amorphous powders.					
30342144	3	26	theme	regression	572:581	arg1	significant					599:609	significant	599:609	significant	599:609	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	26	theme	regression	572:581	arg1	0.03 MPa					730:737	0.03 MPa	730:737	0.03 MPa	730:737	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	26	theme	regression	572:581	arg1	20 °C					720:724	20 °C	720:724	20 °C	720:724	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	26	theme	regression	572:581	arg1	pH 6.5					712:717	pH 6.5	712:717	pH 6.5	712:717	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	26	theme	regression	572:581	arg1	model					583:587	The constructed regression model	556:587	The constructed regression model	556:587	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	27	dep	significant	599:609	arg1	significant					599:609	significant	599:609	significant	599:609	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	27	dep	significant	599:609	arg1	0.03 MPa					730:737	0.03 MPa	730:737	0.03 MPa	730:737	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	27	dep	significant	599:609	arg1	20 °C					720:724	20 °C	720:724	20 °C	720:724	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	27	dep	significant	599:609	arg1	pH 6.5					712:717	pH 6.5	712:717	pH 6.5	712:717	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	27	dep	significant	599:609	arg1	model					583:587	The constructed regression model	556:587	The constructed regression model	556:587	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	2	28	theme	ultrafiltration	451:465	arg1	pH					467:468	the ultrafiltration pH	447:468	the ultrafiltration pH	447:468	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	29	theme	Box-Behnken	339:349	arg1	BBD					359:361	BBD	359:361	BBD	359:361	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	29	theme	Box-Behnken	339:349	arg1	experiments					326:336	single-factor experiments	312:336	single-factor experiments	312:336	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	29	theme	Box-Behnken	339:349	arg1	design					351:356	Box-Behnken design	339:356	Box-Behnken design (BBD)	339:362	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	4	30	theme	CYP	774:776	arg1	rate					789:792	a CYP extraction rate	772:792	a CYP extraction rate of 88.7%	772:801	Under these optimal conditions, a CYP extraction rate of 88.7% was achieved.					
30342144	1	31	theme	macromolecular	222:235	arg1	solutions					237:245	macromolecular solutions	222:245	macromolecular solutions	222:245	Ultrafiltration is a separation process for purifying and concentrating macromolecular solutions.					
30342144	0	32	theme	ultrafiltration-assisted	20:43	arg1	extraction					45:54	the ultrafiltration-assisted extraction	16:54	the ultrafiltration-assisted extraction of Chinese yam polysaccharide	16:84	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	9	33	theme	cell	1450:1453	arg1	growth					1455:1460	BGC-823 cell growth	1442:1460	BGC-823 cell growth	1442:1460	Moreover, CYP exhibited a relatively strong inhibitory effect on BGC-823 cell growth.					
30342144	5	34	theme	CYP	943:945	arg1	composition					928:938	the monosaccharide composition	909:938	the monosaccharide composition of CYP	909:945	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	34	theme	CYP	943:945	arg1	glucose					974:980	50.8% glucose	968:980	50.8% glucose	968:980	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	35	theme	%	987:987	arg1	mannose					989:995	24.2% mannose	983:995	24.2% mannose	983:995	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	36	theme	%	1005:1005	arg1	galactose					1007:1015	11.8% galactose	1001:1015	11.8% galactose	1001:1015	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	8	37	theme	radicals	1367:1374	arg1	scavenging					1299:1308	the scavenging	1295:1308	the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals	1295:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	3	38	theme	constructed	560:570	arg1	significant					599:609	significant	599:609	significant	599:609	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	38	theme	constructed	560:570	arg1	0.03 MPa					730:737	0.03 MPa	730:737	0.03 MPa	730:737	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	38	theme	constructed	560:570	arg1	20 °C					720:724	20 °C	720:724	20 °C	720:724	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	38	theme	constructed	560:570	arg1	pH 6.5					712:717	pH 6.5	712:717	pH 6.5	712:717	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	38	theme	constructed	560:570	arg1	model					583:587	The constructed regression model	556:587	The constructed regression model	556:587	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	9	39	theme	BGC-823	1442:1448	arg1	growth					1455:1460	BGC-823 cell growth	1442:1460	BGC-823 cell growth	1442:1460	Moreover, CYP exhibited a relatively strong inhibitory effect on BGC-823 cell growth.					
30342144	5	40	theme	anion	841:845	arg1	exchange					847:854	anion exchange	841:854	anion exchange (DE-52)	841:862	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	40	theme	anion	841:845	arg1	DE-52					857:861	DE-52	857:861	DE-52	857:861	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	2	41	theme	pressure	487:494	arg1	effects					436:442	the effects	432:442	the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP)	432:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	8	42	theme	hydroxyl	1328:1335	arg1	radicals					1337:1344	hydroxyl radicals	1328:1344	hydroxyl radicals	1328:1344	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	2	43	theme	opposita	275:282	arg1	yam					260:262	Baiyu yam	254:262	Baiyu yam (Dioscorea opposita Thunb)	254:289	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	43	theme	opposita	275:282	arg1	Thunb					284:288	Dioscorea opposita Thunb	265:288	Dioscorea opposita Thunb	265:288	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	5	44	theme	size-exclusion	868:881	arg1	columns					900:906	size-exclusion (Sephadex G-100) columns	868:906	size-exclusion (Sephadex G-100) columns	868:906	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	0	45	theme	extraction	45:54	arg1	Optimization					0:11	Optimization	0:11	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide	0:84	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	9	46	from	effect	1432:1437	arg1	growth					1455:1460	BGC-823 cell growth	1442:1460	BGC-823 cell growth	1442:1460	Moreover, CYP exhibited a relatively strong inhibitory effect on BGC-823 cell growth.					
30342144	3	47	theme	extraction	653:662	arg1	conditions					664:673	the optimal ultrafiltration-assisted extraction conditions	616:673	the optimal ultrafiltration-assisted extraction conditions	616:673	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	47	theme	extraction	653:662	arg1	following					701:709	the following	697:709	the following	697:709	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	2	48	theme	Dioscorea	265:273	arg1	yam					260:262	Baiyu yam	254:262	Baiyu yam (Dioscorea opposita Thunb)	254:289	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	48	theme	Dioscorea	265:273	arg1	Thunb					284:288	Dioscorea opposita Thunb	265:288	Dioscorea opposita Thunb	265:288	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	8	49	theme	radicals	1318:1325	arg1	scavenging					1299:1308	the scavenging	1295:1308	the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals	1295:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	8	50	theme	antioxidant	1261:1271	arg1	activities					1273:1282	antioxidant activities	1261:1282	antioxidant activities	1261:1282	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	8	50	theme	antioxidant	1261:1271	arg1	scavenging					1299:1308	the scavenging	1295:1308	the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals	1295:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	2	51	theme	temperature	471:481	arg1	effects					436:442	the effects	432:442	the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP)	432:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	8	52	theme	superoxide	1350:1359	arg1	radicals					1367:1374	superoxide anion radicals	1350:1374	superoxide anion radicals	1350:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	0	53	theme	polysaccharide	71:84	arg1	extraction					45:54	the ultrafiltration-assisted extraction	16:54	the ultrafiltration-assisted extraction of Chinese yam polysaccharide	16:84	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	6	54	theme	characteristic	1104:1117	arg1	peaks					1130:1134	the characteristic absorption peaks	1100:1134	the characteristic absorption peaks of the polysaccharides	1100:1157	Fourier transform infrared (FT-IR) spectroscopy characterization of CYP confirmed the characteristic absorption peaks of the polysaccharides.					
30342144	0	55	theme	Chinese	59:65	arg1	polysaccharide					71:84	Chinese yam polysaccharide	59:84	Chinese yam polysaccharide	59:84	Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity.					
30342144	2	56	theme	Baiyu	254:258	arg1	yam					260:262	Baiyu yam	254:262	Baiyu yam (Dioscorea opposita Thunb)	254:289	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	56	theme	Baiyu	254:258	arg1	material					302:309	the raw material	294:309	the raw material	294:309	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	56	theme	Baiyu	254:258	arg1	Thunb					284:288	Dioscorea opposita Thunb	265:288	Dioscorea opposita Thunb	265:288	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	5	57	theme	11.8	1001:1004	arg1	%					1005:1005	%	1005:1005	%	1005:1005	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	58	theme	24.2	983:986	arg1	%					987:987	%	987:987	%	987:987	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	59	theme	%	972:972	arg1	composition					928:938	the monosaccharide composition	909:938	the monosaccharide composition of CYP	909:945	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	59	theme	%	972:972	arg1	glucose					974:980	50.8% glucose	968:980	50.8% glucose	968:980	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	3	60	theme	optimal	620:626	arg1	conditions					664:673	the optimal ultrafiltration-assisted extraction conditions	616:673	the optimal ultrafiltration-assisted extraction conditions	616:673	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	60	theme	optimal	620:626	arg1	following					701:709	the following	697:709	the following	697:709	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	8	61	theme	DPPH	1313:1316	arg1	radicals					1318:1325	DPPH radicals	1313:1325	DPPH radicals	1313:1325	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	4	62	theme	extraction	778:787	arg1	rate					789:792	a CYP extraction rate	772:792	a CYP extraction rate of 88.7%	772:801	Under these optimal conditions, a CYP extraction rate of 88.7% was achieved.					
30342144	3	63	theme	ultrafiltration-assisted	628:651	arg1	conditions					664:673	the optimal ultrafiltration-assisted extraction conditions	616:673	the optimal ultrafiltration-assisted extraction conditions	616:673	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	3	63	theme	ultrafiltration-assisted	628:651	arg1	following					701:709	the following	697:709	the following	697:709	The constructed regression model is highly significant, and the optimal ultrafiltration-assisted extraction conditions were determined to be the following: pH 6.5, 20 °C and 0.03 MPa.					
30342144	5	64	theme	monosaccharide	913:926	arg1	composition					928:938	the monosaccharide composition	909:938	the monosaccharide composition of CYP	909:945	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	5	64	theme	monosaccharide	913:926	arg1	glucose					974:980	50.8% glucose	968:980	50.8% glucose	968:980	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	8	65	theme	radicals	1337:1344	arg1	scavenging					1299:1308	the scavenging	1295:1308	the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals	1295:1374	CYP also exhibited antioxidant activities, including the scavenging of DPPH radicals, hydroxyl radicals and superoxide anion radicals.					
30342144	2	66	theme	Chinese	522:528	arg1	CYP					550:552	CYP	550:552	CYP	550:552	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	2	66	theme	Chinese	522:528	arg1	polysaccharide					534:547	Chinese yam polysaccharide	522:547	Chinese yam polysaccharide (CYP)	522:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	5	67	theme	Sephadex	884:891	arg1	columns					900:906	size-exclusion (Sephadex G-100) columns	868:906	size-exclusion (Sephadex G-100) columns	868:906	After purification with anion exchange (DE-52) and size-exclusion (Sephadex G-100) columns, the monosaccharide composition of CYP was determined to be 50.8% glucose, 24.2% mannose and 11.8% galactose.					
30342144	7	68	theme	powders	1233:1239	arg1	typical					1212:1218	typical	1212:1218	typical	1212:1218	The microstructure of CYP exhibited characteristics typical of amorphous powders.					
30342144	2	69	theme	extraction	503:512	arg1	rate					514:517	the extraction rate	499:517	the extraction rate of Chinese yam polysaccharide (CYP)	499:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	4	70	theme	%	801:801	arg1	rate					789:792	a CYP extraction rate	772:792	a CYP extraction rate of 88.7%	772:801	Under these optimal conditions, a CYP extraction rate of 88.7% was achieved.					
30342144	2	71	from	effects	436:442	arg1	rate					514:517	the extraction rate	499:517	the extraction rate of Chinese yam polysaccharide (CYP)	499:553	Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box-Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the ultrafiltration pH, temperature and pressure on the extraction rate of Chinese yam polysaccharide (CYP).					
30342144	1	72	theme	separation	171:180	arg1	Ultrafiltration					150:164	Ultrafiltration	150:164	Ultrafiltration	150:164	Ultrafiltration is a separation process for purifying and concentrating macromolecular solutions.					
30342144	1	72	theme	separation	171:180	arg1	process					182:188	a separation process	169:188	a separation process for purifying and concentrating macromolecular solutions	169:245	Ultrafiltration is a separation process for purifying and concentrating macromolecular solutions.					
30342144	9	73	theme	strong	1414:1419	arg1	effect					1432:1437	a relatively strong inhibitory effect	1401:1437	a relatively strong inhibitory effect on BGC-823 cell growth	1401:1460	Moreover, CYP exhibited a relatively strong inhibitory effect on BGC-823 cell growth.					
29360545	3	0	from	galactose	354:362	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	0	from	galactose	354:362	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	0	from	galactose	354:362	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	0	1	from	characterization	11:26	arg1	ginger					76:81	ginger	76:81	ginger	76:81	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	0	2	from	ginger	76:81	arg1	polysaccharide					56:69	polysaccharide	56:69	polysaccharide from ginger	56:81	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	0	2	from	ginger	76:81	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	0	2	from	ginger	76:81	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	3	3	from	galactose	438:446	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	3	from	galactose	438:446	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	3	from	galactose	438:446	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	4	theme	4.78	468:471	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	5	from	glucose	342:348	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	5	from	glucose	342:348	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	5	from	glucose	342:348	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	6	theme	molar	453:457	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	7	theme	GP2	324:326	arg1	mannose					333:339	mannose	333:339	mannose	333:339	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	7	theme	GP2	324:326	arg1	proportion					302:311	proportion	302:311	proportion	302:311	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	7	theme	GP2	324:326	arg1	composition					286:296	The composition	282:296	The composition	282:296	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	5	8	theme	sulfuric	661:668	arg1	groups					675:680	sulfuric acid groups	661:680	sulfuric acid groups	661:680	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	0	9	from	activity	44:51	arg1	ginger					76:81	ginger	76:81	ginger	76:81	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	3	10	from	mannose	417:423	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	10	from	mannose	417:423	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	10	from	mannose	417:423	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	1	11	theme	ginger	99:104	arg1	polysaccharide					106:119	ginger polysaccharide 1	99:121	ginger polysaccharide 1 (GP1)	99:127	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	11	theme	ginger	99:104	arg1	GP1					124:126	GP1	124:126	GP1	124:126	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	11	theme	ginger	99:104	arg1	components					88:97	Two components	84:97	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2)	84:161	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	0	12	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	0	13	theme	antioxidant	32:42	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	3	14	from	glucose	426:432	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	14	from	glucose	426:432	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	14	from	glucose	426:432	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	5	15	theme	oxidation	698:706	arg1	resistance					708:717	a high oxidation resistance	691:717	a high oxidation resistance	691:717	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	5	16	theme	high	693:696	arg1	resistance					708:717	a high oxidation resistance	691:717	a high oxidation resistance	691:717	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	3	17	theme	molar	369:373	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	18	dep	ratio	459:463	arg1	16.70					474:478	16.70	474:478	16.70	474:478	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	18	dep	ratio	459:463	arg1	16.75					488:492	16.75	488:492	16.75	488:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	18	dep	ratio	459:463	arg1	61.77					481:485	61.77	481:485	61.77	481:485	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	5	19	theme	acid	670:673	arg1	groups					675:680	sulfuric acid groups	661:680	sulfuric acid groups	661:680	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	5	20	contain	has	687:689	arg1	GP2					648:650	GP2	648:650	GP2	648:650	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	5	20	contain	has	687:689	arg2	resistance					708:717	a high oxidation resistance	691:717	a high oxidation resistance	691:717	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	3	21	theme	2.80	397:400	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	21	theme	2.80	397:400	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	22	from	mannose	333:339	arg1	arabinose					406:414	arabinose	406:414	arabinose	406:414	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	22	from	mannose	333:339	arg1	ratio					459:463	a molar ratio	451:463	a molar ratio of 4.78: 16.70: 61.77: 16.75	451:492	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	22	from	mannose	333:339	arg1	ratio					375:379	a molar ratio	367:379	a molar ratio of 4.96: 92.24: 2.80	367:400	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	2	23	theme	molecular	207:215	arg1	weights					217:223	the molecular weights	203:223	the molecular weights of GP1 and GP2	203:238	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	2	23	theme	molecular	207:215	arg1	6128 Da					245:251	6128 Da	245:251	6128 Da	245:251	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	0	24	theme	polysaccharide	56:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	0	24	theme	polysaccharide	56:69	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of polysaccharide from ginger.					
29360545	5	25	contain	contains	652:659	arg1	GP2					648:650	GP2	648:650	GP2	648:650	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	5	25	contain	contains	652:659	arg2	groups					675:680	sulfuric acid groups	661:680	sulfuric acid groups	661:680	GP2 contains sulfuric acid groups, and has a high oxidation resistance, its structure is more evacuated and messy.					
29360545	1	26	dep	components	88:97	arg1	polysaccharide					106:119	ginger polysaccharide 1	99:121	ginger polysaccharide 1 (GP1)	99:127	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	26	dep	components	88:97	arg1	GP1					124:126	GP1	124:126	GP1	124:126	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	26	dep	components	88:97	arg1	components					88:97	Two components	84:97	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2)	84:161	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	26	dep	components	88:97	arg1	polysaccharide					140:153	ginger polysaccharide 2	133:155	ginger polysaccharide 2 (GP2)	133:161	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	26	dep	components	88:97	arg1	GP2					158:160	GP2	158:160	GP2	158:160	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	3	27	theme	homopolysaccharide	558:575	arg1	kind					550:553	a kind	548:553	a kind of homopolysaccharide	548:575	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	27	theme	homopolysaccharide	558:575	arg1	GP2					535:537	GP2	535:537	GP2	535:537	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	27	theme	homopolysaccharide	558:575	arg1	GP1					527:529	GP1	527:529	GP1	527:529	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	2	28	theme	GP2	236:238	arg1	weights					217:223	the molecular weights	203:223	the molecular weights of GP1 and GP2	203:238	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	2	28	theme	GP2	236:238	arg1	6128 Da					245:251	6128 Da	245:251	6128 Da	245:251	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	1	29	theme	ginger	133:138	arg1	components					88:97	Two components	84:97	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2)	84:161	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	29	theme	ginger	133:138	arg1	polysaccharide					140:153	ginger polysaccharide 2	133:155	ginger polysaccharide 2 (GP2)	133:161	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	1	29	theme	ginger	133:138	arg1	GP2					158:160	GP2	158:160	GP2	158:160	Two components ginger polysaccharide 1 (GP1) and ginger polysaccharide 2 (GP2) were extracted.					
29360545	3	30	theme	GP1	316:318	arg1	mannose					333:339	mannose	333:339	mannose	333:339	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	30	theme	GP1	316:318	arg1	proportion					302:311	proportion	302:311	proportion	302:311	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	3	30	theme	GP1	316:318	arg1	composition					286:296	The composition	282:296	The composition	282:296	The composition and proportion of GP1 and GP2 were mannose, glucose and galactose in a molar ratio of 4.96: 92.24: 2.80 and arabinose, mannose, glucose and galactose in a molar ratio of 4.78: 16.70: 61.77: 16.75, respectively, illustrating that GP1 and GP2 were not a kind of homopolysaccharide.					
29360545	4	31	contain	has	582:584	arg2	structure					600:608	a three-helix structure	586:608	a three-helix structure	586:608	GP1 has a three-helix structure, and the structure is closely linked.					
29360545	4	31	contain	has	582:584	arg1	GP1					578:580	GP1	578:580	GP1	578:580	GP1 has a three-helix structure, and the structure is closely linked.					
29360545	2	32	theme	GP1	228:230	arg1	weights					217:223	the molecular weights	203:223	the molecular weights of GP1 and GP2	203:238	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	2	32	theme	GP1	228:230	arg1	6128 Da					245:251	6128 Da	245:251	6128 Da	245:251	The results showed that the molecular weights of GP1 and GP2 were 6128 Da and 12,619 Da, respectively.					
29360545	4	33	theme	three-helix	588:598	arg1	structure					600:608	a three-helix structure	586:608	a three-helix structure	586:608	GP1 has a three-helix structure, and the structure is closely linked.					
31801722	1	0	from	nigricans	232:240	arg1	EPS					213:215	EPS	213:215	EPS	213:215	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	0	from	nigricans	232:240	arg1	substitution					152:163	substitution	152:163	substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans	152:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	0	from	nigricans	232:240	arg1	exopolysaccharide					194:210	sulfated exopolysaccharide	185:210	sulfated exopolysaccharide (EPS) from Rhizopus nigricans	185:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	10	1	theme	direct	1993:1998	arg1	impact					2000:2005	a direct impact	1991:2005	a direct impact	1991:2005	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	1	2	theme	sulfate	250:256	arg1	modification					258:269	sulfate modification	250:269	sulfate modification	250:269	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	6	3	theme	Infrared	1082:1089	arg1	analysis					1100:1107	Infrared spectrum analysis	1082:1107	Infrared spectrum analysis	1082:1107	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	8	4	theme	anti-tumor	1509:1518	arg1	activity					1520:1527	a lower anti-tumor activity	1501:1527	a lower anti-tumor activity than SEPS-1 (P < 0.05)	1501:1550	At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 (P < 0.05).					
31801722	9	5	theme	%	1911:1911	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	5	theme	%	1911:1911	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	10	6	theme	composition	1944:1954	arg1	ratio					1956:1960	The composition ratio	1940:1960	The composition ratio of the esterifying agent	1940:1985	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	3	7	theme	infrared	556:563	arg1	analysis					574:581	infrared spectrum analysis	556:581	infrared spectrum analysis	556:581	The sulfate groups binding with EPS were analyzed with infrared spectrum analysis.					
31801722	7	8	theme	inhibitory	1319:1328	arg1	effects					1330:1336	stronger inhibitory effects	1310:1336	stronger inhibitory effects on the proliferation of HCT 116 cells	1310:1374	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	1	9	theme	group	176:180	arg1	substitution					152:163	substitution	152:163	substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans	152:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	10	10	theme	esterifying	2098:2108	arg1	agent					2110:2114	the esterifying agent	2094:2114	the esterifying agent	2094:2114	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	6	11	theme	absorption	1150:1159	arg1	peak					1161:1164	the S=O stretching vibration absorption peak	1121:1164	the S=O stretching vibration absorption peak of -OSO3-	1121:1174	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	1	12	theme	sulfated	185:192	arg1	EPS					213:215	EPS	213:215	EPS	213:215	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	12	theme	sulfated	185:192	arg1	exopolysaccharide					194:210	sulfated exopolysaccharide	185:210	sulfated exopolysaccharide (EPS) from Rhizopus nigricans	185:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	13	from	effect	117:122	arg1	substitution					152:163	substitution	152:163	substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans	152:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	4	14	theme	EPS	642:644	arg1	sulfate					646:652	EPS sulfate	642:652	EPS sulfate (SEPS)	642:659	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	4	14	theme	EPS	642:644	arg1	SEPS					655:658	SEPS	655:658	SEPS	655:658	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	11	15	theme	antitumor	2161:2169	arg1	activity					2171:2178	its antitumor activity	2157:2178	its antitumor activity	2157:2178	Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.					
31801722	6	16	theme	vibration	1140:1148	arg1	peak					1161:1164	the S=O stretching vibration absorption peak	1121:1164	the S=O stretching vibration absorption peak of -OSO3-	1121:1174	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	6	17	theme	sulfate	1221:1227	arg1	group					1229:1233	the sulfate group	1217:1233	the sulfate group	1217:1233	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	9	18	theme	stronger	1572:1579	arg1	effect					1595:1600	stronger pro-apoptotic effect	1572:1600	stronger pro-apoptotic effect	1572:1600	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	6	19	theme	S=O	1125:1127	arg1	peak					1161:1164	the S=O stretching vibration absorption peak	1121:1164	the S=O stretching vibration absorption peak of -OSO3-	1121:1174	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	9	20	theme	early	1800:1804	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	21	theme	HCT	704:706	arg1	cells					712:716	human colon cancer HCT 116 cells	685:716	human colon cancer HCT 116 cells	685:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	4	22	theme	cells	712:716	arg1	proliferation					668:680	the proliferation	664:680	the proliferation of human colon cancer HCT 116 cells	664:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	10	23	contain	has	1987:1989	arg1	ratio					1956:1960	The composition ratio	1940:1960	The composition ratio of the esterifying agent	1940:1985	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	10	23	contain	has	1987:1989	arg2	impact					2000:2005	a direct impact	1991:2005	a direct impact	1991:2005	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	1	24	theme	composition	131:141	arg1	ratio					143:147	the composition ratio	127:147	the composition ratio	127:147	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	10	25	from	impact	2000:2005	arg1	degree					2014:2019	the degree	2010:2019	the degree of substitution of EPS	2010:2042	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	10	26	theme	esterifying	1969:1979	arg1	agent					1981:1985	the esterifying agent	1965:1985	the esterifying agent	1965:1985	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	1	27	dep	explore	105:111	arg1	affects					271:277	affects	271:277	affects the anti-tumor activity of EPS	271:308	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	0	28	theme	Rhizopus	71:78	arg1	nigricans					80:88	Rhizopus nigricans	71:88	Rhizopus nigricans	71:88	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	4	29	theme	colon	691:695	arg1	cells					712:716	human colon cancer HCT 116 cells	685:716	human colon cancer HCT 116 cells	685:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	1	30	from	substitution	152:163	arg1	nigricans					232:240	Rhizopus nigricans	223:240	Rhizopus nigricans	223:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	30	from	substitution	152:163	arg1	EPS					213:215	EPS	213:215	EPS	213:215	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	1	30	from	substitution	152:163	arg1	exopolysaccharide					194:210	sulfated exopolysaccharide	185:210	sulfated exopolysaccharide (EPS) from Rhizopus nigricans	185:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	5	31	theme	composition	868:878	arg1	ratios					880:885	the composition ratios	864:885	the composition ratios of 1:1 and 2:1	864:900	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	9	32	theme	%	1836:1836	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	32	theme	%	1836:1836	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	11	33	theme	substitution	2240:2251	arg1	degree					2230:2235	the degree	2226:2235	the degree of substitution	2226:2251	Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.					
31801722	11	34	theme	Sulfate	2117:2123	arg1	modification					2125:2136	Sulfate modification	2117:2136	Sulfate modification of EPS	2117:2143	Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.					
31801722	5	35	theme	%	942:942	arg1	substitution					922:933	sulfate substitution	914:933	sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively	914:984	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	2	36	theme	sulfate	444:450	arg1	substitution					452:463	sulfate substitution	444:463	sulfate substitution	444:463	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	5	37	from	ratios	880:885	arg1	EPS					857:859	EPS	857:859	EPS at the composition ratios of 1:1 and 2:1	857:900	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	5	37	from	ratios	880:885	arg1	agent					847:851	The esterifying agent	831:851	The esterifying agent	831:851	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	9	38	theme	late	1853:1856	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	39	theme	V-FITC/PI	731:739	arg1	staining					748:755	annexin V-FITC/PI double staining	723:755	annexin V-FITC/PI double staining	723:755	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	0	40	theme	antitumor	17:25	arg1	activity					27:34	antitumor activity	17:34	antitumor activity	17:34	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	5	41	theme	sulfate	914:920	arg1	substitution					922:933	sulfate substitution	914:933	sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively	914:984	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	2	42	theme	chloride	478:485	arg1	turbidimetry					487:498	barium chloride turbidimetry	471:498	barium chloride turbidimetry	471:498	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	2	43	theme	esterification	406:419	arg1	ratio					421:425	esterification ratio	406:425	esterification ratio	406:425	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	1	44	theme	EPS	306:308	arg1	activity					294:301	the anti-tumor activity	279:301	the anti-tumor activity of EPS	279:308	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	5	45	theme	%	961:961	arg1	substitution					922:933	sulfate substitution	914:933	sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively	914:984	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	9	46	theme	early	1695:1699	arg1	cells					1711:1715	early apoptotic cells	1695:1715	early apoptotic cells	1695:1715	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	5	47	theme	agent	1064:1068	arg1	ratio					1039:1043	the ratio	1035:1043	the ratio of the esterifying agent (P < 0.05)	1035:1079	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	2	48	theme	acid-pyridine	344:356	arg1	method					358:363	a chlorosulfonic acid-pyridine method	327:363	a chlorosulfonic acid-pyridine method	327:363	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	0	49	theme	exopolysaccharide	48:64	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation	0:11	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	0	49	theme	exopolysaccharide	48:64	arg1	activity					27:34	antitumor activity	17:34	antitumor activity	17:34	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	5	50	theme	P	1071:1071	arg1	agent					1064:1068	the esterifying agent	1048:1068	the esterifying agent (P < 0.05)	1048:1079	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	5	50	theme	P	1071:1071	arg1	<					1073:1073	P < 0.05	1071:1078	P < 0.05	1071:1078	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	2	51	from	effect	396:401	arg1	degree					434:439	the degree	430:439	the degree of sulfate substitution	430:463	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	9	52	theme	cells	1711:1715	arg1	ratio					1686:1690	the ratio	1682:1690	the ratio of early apoptotic cells and necrotic cells	1682:1734	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	53	used	used	761:764	arg2	staining					748:755	annexin V-FITC/PI double staining	723:755	annexin V-FITC/PI double staining	723:755	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	10	54	theme	agent	2110:2114	arg1	ratio					2085:2089	the ratio	2081:2089	the ratio of the esterifying agent	2081:2114	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	9	55	theme	necrotic	1721:1728	arg1	cells					1730:1734	necrotic cells	1721:1734	necrotic cells	1721:1734	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	56	theme	pro-apoptotic	780:792	arg1	effect					794:799	the pro-apoptotic effect	776:799	the pro-apoptotic effect of SEPS in the cells	776:820	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	5	57	theme	esterifying	835:845	arg1	agent					847:851	The esterifying agent	831:851	The esterifying agent	831:851	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	4	58	theme	inhibitory	621:630	arg1	effect					632:637	the inhibitory effect	617:637	the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells	617:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	9	59	theme	%	1901:1901	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	59	theme	%	1901:1901	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	8	60	theme	P	1542:1542	arg1	SEPS-1					1534:1539	SEPS-1	1534:1539	SEPS-1 (P < 0.05)	1534:1550	At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 (P < 0.05).					
31801722	8	60	theme	P	1542:1542	arg1	<					1544:1544	P < 0.05	1542:1549	P < 0.05	1542:1549	At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 (P < 0.05).					
31801722	7	61	theme	cells	1370:1374	arg1	proliferation					1345:1357	the proliferation	1341:1357	the proliferation of HCT 116 cells	1341:1374	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	10	62	theme	EPS	2040:2042	arg1	substitution					2024:2035	substitution	2024:2035	substitution of EPS	2024:2042	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	7	63	theme	HCT	1362:1364	arg1	cells					1370:1374	HCT 116 cells	1362:1374	HCT 116 cells	1362:1374	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	10	64	theme	substitution	2024:2035	arg1	degree					2014:2019	the degree	2010:2019	the degree of substitution of EPS	2010:2042	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	8	65	theme	lower	1503:1507	arg1	activity					1520:1527	a lower anti-tumor activity	1501:1527	a lower anti-tumor activity than SEPS-1 (P < 0.05)	1501:1550	At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 (P < 0.05).					
31801722	6	66	theme	-OSO3-	1169:1174	arg1	peak					1161:1164	the S=O stretching vibration absorption peak	1121:1164	the S=O stretching vibration absorption peak of -OSO3-	1121:1174	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	1	67	theme	sulfate	168:174	arg1	group					176:180	sulfate group	168:180	sulfate group	168:180	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	9	68	theme	apoptotic	1701:1709	arg1	cells					1711:1715	early apoptotic cells	1695:1715	early apoptotic cells	1695:1715	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	7	69	theme	stronger	1310:1317	arg1	effects					1330:1336	stronger inhibitory effects	1310:1336	stronger inhibitory effects on the proliferation of HCT 116 cells	1310:1374	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	3	70	theme	spectrum	565:572	arg1	analysis					574:581	infrared spectrum analysis	556:581	infrared spectrum analysis	556:581	The sulfate groups binding with EPS were analyzed with infrared spectrum analysis.					
31801722	9	71	theme	mg/mL	1780:1784	arg1	SEPS-1					1786:1791	0.06, 0.08 and 0.10 mg/mL SEPS-1	1760:1791	0.06, 0.08 and 0.10 mg/mL SEPS-1	1760:1791	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	2	72	theme	chlorosulfonic	329:342	arg1	method					358:363	a chlorosulfonic acid-pyridine method	327:363	a chlorosulfonic acid-pyridine method	327:363	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	0	73	from	nigricans	80:88	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation	0:11	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	0	73	from	nigricans	80:88	arg1	activity					27:34	antitumor activity	17:34	antitumor activity	17:34	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	6	74	theme	stretching	1129:1138	arg1	peak					1161:1164	the S=O stretching vibration absorption peak	1121:1164	the S=O stretching vibration absorption peak of -OSO3-	1121:1174	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	4	75	theme	sulfate	646:652	arg1	effect					632:637	the inhibitory effect	617:637	the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells	617:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	4	76	used	used	600:603	arg2	assay					590:594	CCK-8 assay	584:594	CCK-8 assay	584:594	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	9	77	theme	pro-apoptotic	1581:1593	arg1	effect					1595:1600	stronger pro-apoptotic effect	1572:1600	stronger pro-apoptotic effect	1572:1600	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	7	78	theme	CCK-8	1270:1274	arg1	assay					1276:1280	CCK-8 assay	1270:1280	CCK-8 assay	1270:1280	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	9	79	theme	apoptotic	1806:1814	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	80	theme	cancer	697:702	arg1	cells					712:716	human colon cancer HCT 116 cells	685:716	human colon cancer HCT 116 cells	685:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	4	81	from	effect	794:799	arg1	cells					816:820	the cells	812:820	the cells	812:820	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	5	82	theme	1:1	890:892	arg1	ratios					880:885	the composition ratios	864:885	the composition ratios of 1:1 and 2:1	864:900	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	10	83	theme	agent	1981:1985	arg1	ratio					1956:1960	The composition ratio	1940:1960	The composition ratio of the esterifying agent	1940:1985	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	2	84	dep	METHODS	311:317	arg1	used					322:325	used	322:325	used a chlorosulfonic acid-pyridine method to modify EPS	322:377	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	2	84	dep	METHODS	311:317	arg1	analyzed					383:390	analyzed	383:390	analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry	383:498	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	4	85	from	effect	632:637	arg1	proliferation					668:680	the proliferation	664:680	the proliferation of human colon cancer HCT 116 cells	664:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	1	86	theme	ratio	143:147	arg1	effect					117:122	the effect	113:122	the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans	113:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	6	87	theme	spectrum	1091:1098	arg1	analysis					1100:1107	Infrared spectrum analysis	1082:1107	Infrared spectrum analysis	1082:1107	Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO3- appeared near 1249 cm-1, indicating that the sulfate group combined with EPS to form sulfate.					
31801722	9	88	theme	%	1829:1829	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	88	theme	%	1829:1829	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	7	89	from	effects	1330:1336	arg1	proliferation					1345:1357	the proliferation	1341:1357	the proliferation of HCT 116 cells	1341:1374	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	4	90	theme	human	685:689	arg1	cells					712:716	human colon cancer HCT 116 cells	685:716	human colon cancer HCT 116 cells	685:716	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	11	91	with	related	2213:2219	arg1	degree					2230:2235	the degree	2226:2235	the degree of substitution	2226:2251	Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.					
31801722	5	92	theme	esterifying	1052:1062	arg1	agent					1064:1068	the esterifying agent	1048:1068	the esterifying agent (P < 0.05)	1048:1079	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	5	92	theme	esterifying	1052:1062	arg1	<					1073:1073	P < 0.05	1071:1078	P < 0.05	1071:1078	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	4	93	theme	double	741:746	arg1	staining					748:755	annexin V-FITC/PI double staining	723:755	annexin V-FITC/PI double staining	723:755	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	2	94	used	used	322:325	arg2	We					319:320	We	319:320	We	319:320	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	5	95	theme	2:1	898:900	arg1	ratios					880:885	the composition ratios	864:885	the composition ratios of 1:1 and 2:1	864:900	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	11	96	theme	EPS	2141:2143	arg1	modification					2125:2136	Sulfate modification	2117:2136	Sulfate modification of EPS	2117:2143	Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.					
31801722	1	97	theme	anti-tumor	283:292	arg1	activity					294:301	the anti-tumor activity	279:301	the anti-tumor activity of EPS	279:308	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	10	98	dep	CONCLUSIONS	1928:1938	arg1	has					1987:1989	has	1987:1989	has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent	1987:2114	CONCLUSIONS The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent.					
31801722	4	99	theme	CCK-8	584:588	arg1	assay					590:594	CCK-8 assay	584:594	CCK-8 assay	584:594	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	9	100	theme	apoptotic	1858:1866	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	4	101	theme	annexin	723:729	arg1	staining					748:755	annexin V-FITC/PI double staining	723:755	annexin V-FITC/PI double staining	723:755	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	2	102	theme	ratio	421:425	arg1	effect					396:401	the effect	392:401	the effect of esterification ratio on the degree of sulfate substitution	392:463	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	0	103	theme	sulfated	39:46	arg1	exopolysaccharide					48:64	sulfated exopolysaccharide	39:64	sulfated exopolysaccharide	39:64	[Preparation and antitumor activity of sulfated exopolysaccharide from Rhizopus nigricans].					
31801722	7	104	theme	P	1435:1435	arg1	mg/L					1429:1432	0.02-0.10 mg/L	1419:1432	0.02-0.10 mg/L (P < 0.05)	1419:1443	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	7	104	theme	P	1435:1435	arg1	<					1437:1437	P < 0.05	1435:1442	P < 0.05	1435:1442	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	2	105	theme	barium	471:476	arg1	turbidimetry					487:498	barium chloride turbidimetry	471:498	barium chloride turbidimetry	471:498	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	9	106	theme	necrotic	1872:1879	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	7	107	theme	mg/L	1429:1432	arg1	range					1410:1414	the concentration range	1392:1414	the concentration range of 0.02-0.10 mg/L (P < 0.05)	1392:1443	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	2	108	theme	substitution	452:463	arg1	degree					434:439	the degree	430:439	the degree of sulfate substitution	430:463	METHODS We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry.					
31801722	4	109	theme	SEPS	804:807	arg1	effect					794:799	the pro-apoptotic effect	776:799	the pro-apoptotic effect of SEPS in the cells	776:820	CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.					
31801722	3	110	theme	sulfate	505:511	arg1	groups					513:518	The sulfate groups	501:518	The sulfate groups binding with EPS	501:535	The sulfate groups binding with EPS were analyzed with infrared spectrum analysis.					
31801722	9	111	theme	%	1846:1846	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	111	theme	%	1846:1846	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	8	112	theme	mg/L	1481:1484	arg1	concentrations					1453:1466	the concentrations	1449:1466	the concentrations of 0.04-0.08 mg/L	1449:1484	At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 (P < 0.05).					
31801722	9	113	theme	%	1894:1894	arg1	rates					1881:1885	late apoptotic and necrotic rates	1853:1885	late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively	1853:1925	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	113	theme	%	1894:1894	arg1	rates					1816:1820	early apoptotic rates	1800:1820	early apoptotic rates of 6.38%, 11.8% and 12.5%	1800:1846	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	5	114	dep	RESULTS	823:829	arg1	EPS					857:859	EPS	857:859	EPS at the composition ratios of 1:1 and 2:1	857:900	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	5	114	dep	RESULTS	823:829	arg1	agent					847:851	The esterifying agent	831:851	The esterifying agent	831:851	RESULTS The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent (P < 0.05).					
31801722	1	115	theme	Rhizopus	223:230	arg1	nigricans					232:240	Rhizopus nigricans	223:240	Rhizopus nigricans	223:240	OBJECTIVE To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from Rhizopus nigricans and how sulfate modification affects the anti-tumor activity of EPS.					
31801722	7	116	theme	concentration	1396:1408	arg1	range					1410:1414	the concentration range	1392:1414	the concentration range of 0.02-0.10 mg/L (P < 0.05)	1392:1443	CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L (P < 0.05).					
31801722	9	117	theme	cells	1730:1734	arg1	ratio					1686:1690	the ratio	1682:1690	the ratio of early apoptotic cells and necrotic cells	1682:1734	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
31801722	9	118	theme	8.04	1897:1900	arg1	%					1901:1901	8.04%	1897:1901	8.04%	1897:1901	SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.					
30925745	0	0	theme	indica	93:98	arg1	L.					100:101	Tamarindus indica L.	82:101	Tamarindus indica L.	82:101	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	7	1	theme	high	1004:1007	arg1	temperature					1009:1019	high temperature	1004:1019	high temperature	1004:1019	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	8	2	theme	processed	1235:1243	arg1	foodstuffs					1245:1254	high-temperature processed foodstuffs	1218:1254	high-temperature processed foodstuffs	1218:1254	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	1	3	theme	hot	213:215	arg1	extraction					223:232	acidic hot water extraction	206:232	acidic hot water extraction	206:232	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	0	4	theme	Tamarindus	82:91	arg1	L.					100:101	Tamarindus indica L.	82:101	Tamarindus indica L.	82:101	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	6	5	theme	oscillatory	753:763	arg1	analysis					765:772	Dynamic oscillatory analysis	745:772	Dynamic oscillatory analysis	745:772	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	1	6	theme	water	217:221	arg1	extraction					223:232	acidic hot water extraction	206:232	acidic hot water extraction	206:232	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	5	7	theme	aqueous	643:649	arg1	solution					651:658	TSP aqueous solution	639:658	TSP aqueous solution at a concentration above 0.5% (w/v)	639:694	Results revealed that TSP aqueous solution at a concentration above 0.5% (w/v) exhibited non-Newtonian shear-thinning behavior.					
30925745	3	8	from	xylose	423:428	arg1	ratio					456:460	a molar ratio	448:460	a molar ratio of 2.9:1.8:1.0	448:475	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	2	9	dep	%	313:313	arg1	acid					322:325	uronic acid	315:325	5.4% uronic acid	310:325	It was composed of 86.2% neutral polysaccharide, 5.4% uronic acid and 1.3% protein.					
30925745	7	10	theme	Apparent	836:843	arg1	viscosities					845:855	Apparent viscosities	836:855	Apparent viscosities	836:855	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	3	11	theme	molar	450:454	arg1	ratio					456:460	a molar ratio	448:460	a molar ratio of 2.9:1.8:1.0	448:475	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	3	12	with	kDa	404:406	arg1	xylose					423:428	xylose	423:428	xylose	423:428	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	3	12	with	kDa	404:406	arg1	galactose					435:443	galactose	435:443	galactose	435:443	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	3	12	with	kDa	404:406	arg1	glucose					414:420	glucose	414:420	glucose	414:420	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	4	13	theme	solutions	567:575	arg1	shear					518:522	steady shear	511:522	steady shear	511:522	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	4	13	theme	solutions	567:575	arg1	properties					541:550	viscoelastic properties	528:550	viscoelastic properties	528:550	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	3	14	theme	molecular	349:357	arg1	kDa					404:406	about 1735 kDa	393:406	about 1735 kDa	393:406	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	3	14	theme	molecular	349:357	arg1	weight					359:364	The molecular weight	345:364	The molecular weight of TSP	345:371	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	7	15	theme	sucrose	1055:1061	arg1	presence					1029:1036	the presence	1025:1036	the presence of salt ions and sucrose	1025:1061	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	7	15	theme	sucrose	1055:1061	arg1	solution					994:1001	acidic solution	987:1001	acidic solution	987:1001	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	7	15	theme	sucrose	1055:1061	arg1	temperature					1009:1019	high temperature	1004:1019	high temperature	1004:1019	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	4	16	theme	aqueous	559:565	arg1	solutions					567:575	TSP aqueous solutions	555:575	TSP aqueous solutions	555:575	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	5	17	theme	non-Newtonian	706:718	arg1	behavior					735:742	non-Newtonian shear-thinning behavior	706:742	non-Newtonian shear-thinning behavior	706:742	Results revealed that TSP aqueous solution at a concentration above 0.5% (w/v) exhibited non-Newtonian shear-thinning behavior.					
30925745	3	18	theme	2.9:1.8:1.0	465:475	arg1	ratio					456:460	a molar ratio	448:460	a molar ratio of 2.9:1.8:1.0	448:475	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	1	19	theme	Tamarindus	170:179	arg1	seed					192:195	seed	192:195	seed	192:195	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	1	19	theme	Tamarindus	170:179	arg1	L.					188:189	Tamarindus indica L.	170:189	Tamarindus indica L.	170:189	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	5	20	theme	shear-thinning	720:733	arg1	behavior					735:742	non-Newtonian shear-thinning behavior	706:742	non-Newtonian shear-thinning behavior	706:742	Results revealed that TSP aqueous solution at a concentration above 0.5% (w/v) exhibited non-Newtonian shear-thinning behavior.					
30925745	1	21	theme	ethanol	238:244	arg1	precipitation					246:258	ethanol precipitation	238:258	ethanol precipitation	238:258	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	2	22	dep	%	284:284	arg1	polysaccharide					294:307	neutral polysaccharide	286:307	86.2% neutral polysaccharide	280:307	It was composed of 86.2% neutral polysaccharide, 5.4% uronic acid and 1.3% protein.					
30925745	3	23	theme	TSP	369:371	arg1	kDa					404:406	about 1735 kDa	393:406	about 1735 kDa	393:406	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	3	23	theme	TSP	369:371	arg1	weight					359:364	The molecular weight	345:364	The molecular weight of TSP	345:371	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	7	24	theme	salt	1041:1044	arg1	ions					1046:1049	salt ions	1041:1049	salt ions	1041:1049	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	8	25	from	applications	1181:1192	arg1	foodstuffs					1245:1254	high-temperature processed foodstuffs	1218:1254	high-temperature processed foodstuffs	1218:1254	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	8	25	from	applications	1181:1192	arg1	beverages					1204:1212	acidic beverages	1197:1212	acidic beverages	1197:1212	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	3	26	from	glucose	414:420	arg1	ratio					456:460	a molar ratio	448:460	a molar ratio of 2.9:1.8:1.0	448:475	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	7	27	theme	acidic	987:992	arg1	solution					994:1001	acidic solution	987:1001	acidic solution	987:1001	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	0	28	theme	Rheological	16:26	arg1	Properties					28:37	Rheological Properties	16:37	Rheological Properties	16:37	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	8	29	dep	processed	1235:1243	arg1	high-temperature					1218:1233	high-temperature	1218:1233	high-temperature	1218:1233	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	0	30	dep	Tamarind	72:79	arg1	L.					100:101	Tamarindus indica L.	82:101	Tamarindus indica L.	82:101	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	4	31	theme	viscoelastic	528:539	arg1	properties					541:550	viscoelastic properties	528:550	viscoelastic properties	528:550	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	0	32	theme	Polysaccharide	42:55	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	0	32	theme	Polysaccharide	42:55	arg1	Properties					28:37	Rheological Properties	16:37	Rheological Properties	16:37	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	7	33	theme	>	954:954	arg1	solution					939:946	an alkaline solution	927:946	an alkaline solution of pH > 10	927:957	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	6	34	theme	%	790:790	arg1	TSP					798:800	10% (w/v) TSP	788:800	10% (w/v) TSP	788:800	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	8	35	contain	possessed	1097:1105	arg1	TSP					1093:1095	TSP	1093:1095	TSP	1093:1095	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	8	35	contain	possessed	1097:1105	arg2	thermo-stability					1135:1150	thermo-stability	1135:1150	thermo-stability	1135:1150	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	8	35	contain	possessed	1097:1105	arg2	pH-resistance					1117:1129	excellent pH-resistance	1107:1129	excellent pH-resistance	1107:1129	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	1	36	theme	indica	181:186	arg1	seed					192:195	seed	192:195	seed	192:195	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	1	36	theme	indica	181:186	arg1	L.					188:189	Tamarindus indica L.	170:189	Tamarindus indica L.	170:189	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	4	37	theme	TSP	555:557	arg1	solutions					567:575	TSP aqueous solutions	555:575	TSP aqueous solutions	555:575	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	5	38	theme	TSP	639:641	arg1	solution					651:658	TSP aqueous solution	639:658	TSP aqueous solution at a concentration above 0.5% (w/v)	639:694	Results revealed that TSP aqueous solution at a concentration above 0.5% (w/v) exhibited non-Newtonian shear-thinning behavior.					
30925745	6	39	theme	10	788:789	arg1	%					790:790	%	790:790	%	790:790	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	1	40	from	seed	192:195	arg1	yield					149:153	high yield	144:153	high yield from tamarind (Tamarindus indica L.) seed (TSP)	144:201	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	6	41	dep	%	790:790	arg1	w/v					793:795	w/v	793:795	w/v	793:795	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	8	42	theme	excellent	1107:1115	arg1	pH-resistance					1117:1129	excellent pH-resistance	1107:1129	excellent pH-resistance	1107:1129	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	6	43	theme	"	823:823	arg1	structure					825:833	a "weak gel" structure	812:833	a "weak gel" structure	812:833	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	7	44	theme	pH	951:952	arg1	>					954:954	pH > 10	951:957	pH > 10	951:957	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	3	45	theme	major	484:488	arg1	monosaccharides					490:504	the major monosaccharides	480:504	the major monosaccharides	480:504	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	7	46	theme	ions	1046:1049	arg1	presence					1029:1036	the presence	1025:1036	the presence of salt ions and sucrose	1025:1061	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	7	46	theme	ions	1046:1049	arg1	solution					994:1001	acidic solution	987:1001	acidic solution	987:1001	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	7	46	theme	ions	1046:1049	arg1	temperature					1009:1019	high temperature	1004:1019	high temperature	1004:1019	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	6	47	theme	gel	820:822	arg1	structure					825:833	a "weak gel" structure	812:833	a "weak gel" structure	812:833	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	7	48	theme	alkaline	930:937	arg1	solution					939:946	an alkaline solution	927:946	an alkaline solution of pH > 10	927:957	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	4	49	dep	shear	518:522	arg1	The					507:509	The	507:509	The	507:509	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	7	50	theme	viscoelastic	861:872	arg1	parameters					874:883	viscoelastic parameters	861:883	viscoelastic parameters	861:883	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	2	51	theme	neutral	286:292	arg1	polysaccharide					294:307	neutral polysaccharide	286:307	86.2% neutral polysaccharide	280:307	It was composed of 86.2% neutral polysaccharide, 5.4% uronic acid and 1.3% protein.					
30925745	4	52	theme	steady	511:516	arg1	shear					518:522	steady shear	511:522	steady shear	511:522	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	5	53	from	concentration	665:677	arg1	solution					651:658	TSP aqueous solution	639:658	TSP aqueous solution at a concentration above 0.5% (w/v)	639:694	Results revealed that TSP aqueous solution at a concentration above 0.5% (w/v) exhibited non-Newtonian shear-thinning behavior.					
30925745	7	54	theme	TSP	888:890	arg1	solutions					892:900	TSP solutions	888:900	TSP solutions	888:900	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	1	55	theme	high	144:147	arg1	yield					149:153	high yield	144:153	high yield from tamarind (Tamarindus indica L.) seed (TSP)	144:201	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30925745	8	56	theme	acidic	1197:1202	arg1	beverages					1204:1212	acidic beverages	1197:1212	acidic beverages	1197:1212	These results indicated that TSP possessed excellent pH-resistance and thermo-stability, which might be suitable for applications in acidic beverages and high-temperature processed foodstuffs.					
30925745	6	57	theme	Dynamic	745:751	arg1	analysis					765:772	Dynamic oscillatory analysis	745:772	Dynamic oscillatory analysis	745:772	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	7	58	theme	solutions	892:900	arg1	viscosities					845:855	Apparent viscosities	836:855	Apparent viscosities	836:855	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	7	58	theme	solutions	892:900	arg1	parameters					874:883	viscoelastic parameters	861:883	viscoelastic parameters	861:883	Apparent viscosities and viscoelastic parameters of TSP solutions decreased drastically in an alkaline solution of pH > 10, but slightly influenced by acidic solution, high temperature and the presence of salt ions and sucrose.					
30925745	4	59	theme	dynamic	598:604	arg1	rheometry					606:614	dynamic rheometry	598:614	dynamic rheometry	598:614	The steady shear and viscoelastic properties of TSP aqueous solutions were investigated by dynamic rheometry.					
30925745	0	60	theme	Tamarind	72:79	arg1	Seed					104:107	Tamarind (Tamarindus indica L.) Seed	72:107	Tamarind (Tamarindus indica L.) Seed	72:107	Composition and Rheological Properties of Polysaccharide Extracted from Tamarind (Tamarindus indica L.) Seed.					
30925745	6	61	theme	weak	815:818	arg1	structure					825:833	a "weak gel" structure	812:833	a "weak gel" structure	812:833	Dynamic oscillatory analysis revealed that 10% (w/v) TSP showed as a "weak gel" structure.					
30925745	2	62	dep	%	334:334	arg1	protein					336:342	protein	336:342	1.3% protein	331:342	It was composed of 86.2% neutral polysaccharide, 5.4% uronic acid and 1.3% protein.					
30925745	2	63	theme	uronic	315:320	arg1	acid					322:325	uronic acid	315:325	5.4% uronic acid	310:325	It was composed of 86.2% neutral polysaccharide, 5.4% uronic acid and 1.3% protein.					
30925745	3	64	from	galactose	435:443	arg1	ratio					456:460	a molar ratio	448:460	a molar ratio of 2.9:1.8:1.0	448:475	The molecular weight of TSP was estimated to be about 1735 kDa, with glucose, xylose, and galactose in a molar ratio of 2.9:1.8:1.0 as the major monosaccharides.					
30925745	1	65	theme	acidic	206:211	arg1	extraction					223:232	acidic hot water extraction	206:232	acidic hot water extraction	206:232	A polysaccharide was extracted in high yield from tamarind (Tamarindus indica L.) seed (TSP) by acidic hot water extraction and ethanol precipitation.					
30040844	5	0	theme	structural	743:752	arg1	details					754:760	the structural details	739:760	the structural details of the mucilage from the micro- down to the nanoscale	739:814	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	5	1	theme	critical	647:654	arg1	CPD					670:672	CPD	670:672	CPD	670:672	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	5	1	theme	critical	647:654	arg1	drying					662:667	critical point drying	647:667	critical point drying (CPD)	647:673	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	4	2	theme	mucilage	572:579	arg1	envelope					581:588	the seed mucilage envelope	563:588	the seed mucilage envelope of selected taxa which produce cellulose mucilage	563:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	0	3	theme	study	79:83	arg1	architecture					33:44	The micro- and nanoscale spatial architecture	0:44	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species	0:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	5	4	theme	point	656:660	arg1	CPD					670:672	CPD	670:672	CPD	670:672	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	5	4	theme	point	656:660	arg1	drying					662:667	critical point drying	647:667	critical point drying (CPD)	647:673	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	2	5	theme	typical	320:326	arg1	polysaccharides					328:342	the typical polysaccharides i.e. cellulose	316:357	the typical polysaccharides i.e. cellulose	316:357	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	4	6	theme	seed	567:570	arg1	envelope					581:588	the seed mucilage envelope	563:588	the seed mucilage envelope of selected taxa which produce cellulose mucilage	563:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	0	7	theme	plant	97:101	arg1	species					103:109	selected plant species	88:109	selected plant species	88:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	4	8	theme	presented	504:512	arg1	study					514:518	the presented study	500:518	the presented study	500:518	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	5	9	theme	drying	662:667	arg1	imaging					714:720	critical point drying (CPD) and scanning electron microscopy (SEM) imaging	647:720	critical point drying (CPD) and scanning electron microscopy (SEM) imaging	647:720	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	2	10	dep	polysaccharides	328:342	arg1	cellulose					349:357	i.e. cellulose	344:357	the typical polysaccharides i.e. cellulose	316:357	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	9	11	theme	mechanical	1488:1497	arg1	impacts					1499:1505	mechanical impacts	1488:1505	mechanical impacts	1488:1505	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	2	12	with	wall	299:302	arg1	polysaccharides					328:342	the typical polysaccharides i.e. cellulose	316:357	the typical polysaccharides i.e. cellulose	316:357	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	2	12	with	wall	299:302	arg1	hemicelluloses					372:385	hemicelluloses	372:385	hemicelluloses	372:385	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	2	12	with	wall	299:302	arg1	all					309:311	all	309:311	all	309:311	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	2	12	with	wall	299:302	arg1	pectins					360:366	pectins	360:366	pectins	360:366	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	0	13	theme	selected	88:95	arg1	species					103:109	selected plant species	88:109	selected plant species	88:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	5	14	theme	microscopy	697:706	arg1	imaging					714:720	critical point drying (CPD) and scanning electron microscopy (SEM) imaging	647:720	critical point drying (CPD) and scanning electron microscopy (SEM) imaging	647:720	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	7	15	theme	mucilaginous	1057:1068	arg1	components					1081:1090	the mucilaginous fibrillary components	1053:1090	the mucilaginous fibrillary components	1053:1090	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	2	16	theme	special	269:275	arg1	type					277:280	a special type	267:280	a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses	267:385	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	4	17	theme	envelope	581:588	arg1	architecture					547:558	the spatial architecture	535:558	the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage	535:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	4	18	theme	cellulose	621:629	arg1	mucilage					631:638	cellulose mucilage	621:638	cellulose mucilage	621:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	8	19	theme	components	1296:1305	arg1	rest					1284:1287	the rest	1280:1287	the rest of the components which were spread between them and/or covered their surface	1280:1365	Cellulose fibrils built a kind of scaffold for the rest of the components which were spread between them and/or covered their surface.					
30040844	3	20	theme	mucilage	420:427	arg1	well-recognized					432:446	well-recognized	432:446	well-recognized	432:446	The chemical composition of the mucilage is well-recognized but its structural organization remains unclear.					
30040844	3	20	theme	mucilage	420:427	arg1	composition					401:411	The chemical composition	388:411	The chemical composition of the mucilage	388:427	The chemical composition of the mucilage is well-recognized but its structural organization remains unclear.					
30040844	0	21	theme	species	103:109	arg1	study					79:83	the seed mucilage-Comparative study	49:83	the seed mucilage-Comparative study of selected plant species	49:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	10	22	theme	seed	1690:1693	arg1	envelope					1704:1711	the seed mucilage envelope	1686:1711	the seed mucilage envelope	1686:1711	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	7	23	dep	long	1115:1118	arg1	thicker					1133:1139	thicker	1133:1139	thicker	1133:1139	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	7	23	dep	long	1115:1118	arg1	unbranched					1121:1130	unbranched	1121:1130	unbranched	1121:1130	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	0	24	theme	micro-	4:9	arg1	architecture					33:44	The micro- and nanoscale spatial architecture	0:44	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species	0:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	5	25	from	micro-	787:792	arg1	details					754:760	the structural details	739:760	the structural details of the mucilage from the micro- down to the nanoscale	739:814	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	7	26	theme	shorter	1173:1179	arg1	polysaccharides					1216:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	7	27	theme	branched	1201:1208	arg1	polysaccharides					1216:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	2	28	theme	wall	299:302	arg1	type					277:280	a special type	267:280	a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses	267:385	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	10	29	theme	special	1535:1541	arg1	composition					1552:1562	special chemical composition	1535:1562	special chemical composition	1535:1562	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	10	30	theme	chemical	1543:1550	arg1	composition					1552:1562	special chemical composition	1535:1562	special chemical composition	1535:1562	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	6	31	from	organization	938:949	arg1	basilicum					1016:1024	Ocimum basilicum	1009:1024	Ocimum basilicum	1009:1024	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	6	31	from	organization	938:949	arg1	thaliana					966:973	Arabidopsis thaliana	954:973	Arabidopsis thaliana	954:973	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	9	32	attach	attached	1395:1402	arg2	fibrils					1382:1388	The cellulose fibrils	1368:1388	The cellulose fibrils	1368:1388	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	9	32	attach	attached	1395:1402	arg1	surface					1416:1422	the seed surface	1407:1422	the seed surface	1407:1422	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	2	33	theme	cell	294:297	arg1	wall					299:302	modified cell wall	285:302	modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses	285:385	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	6	34	contain	had	842:844	arg2	structure					864:872	a visibly spatial structure	846:872	a visibly spatial structure which differed between the studied taxa	846:912	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	6	34	contain	had	842:844	arg1	mucilage					821:828	The mucilage	817:828	The mucilage	817:828	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	10	35	theme	wall	1589:1592	arg1	composition					1552:1562	special chemical composition	1535:1562	special chemical composition	1535:1562	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	10	35	theme	wall	1589:1592	arg1	architecture					1518:1529	loose architecture	1512:1529	loose architecture	1512:1529	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	0	36	theme	nanoscale	15:23	arg1	architecture					33:44	The micro- and nanoscale spatial architecture	0:44	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species	0:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	4	37	theme	spatial	539:545	arg1	architecture					547:558	the spatial architecture	535:558	the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage	535:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	5	38	theme	electron	688:695	arg1	microscopy					697:706	scanning electron microscopy	679:706	scanning electron microscopy (SEM)	679:712	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	5	38	theme	electron	688:695	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	6	39	from	arrangement	994:1004	arg1	basilicum					1016:1024	Ocimum basilicum	1009:1024	Ocimum basilicum	1009:1024	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	6	39	from	arrangement	994:1004	arg1	thaliana					966:973	Arabidopsis thaliana	954:973	Arabidopsis thaliana	954:973	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	2	40	theme	modified	285:292	arg1	wall					299:302	modified cell wall	285:302	modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses	285:385	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	9	41	theme	seed	1411:1414	arg1	surface					1416:1422	the seed surface	1407:1422	the seed surface	1407:1422	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	4	42	theme	taxa	602:605	arg1	envelope					581:588	the seed mucilage envelope	563:588	the seed mucilage envelope of selected taxa which produce cellulose mucilage	563:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	9	43	theme	mucilage	1463:1470	arg1	envelope					1472:1479	the mucilage envelope	1459:1479	the mucilage envelope	1459:1479	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	9	44	theme	cellulose	1372:1380	arg1	fibrils					1382:1388	The cellulose fibrils	1368:1388	The cellulose fibrils	1368:1388	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	3	45	theme	structural	456:465	arg1	organization					467:478	its structural organization	452:478	its structural organization	452:478	The chemical composition of the mucilage is well-recognized but its structural organization remains unclear.					
30040844	3	46	theme	chemical	392:399	arg1	well-recognized					432:446	well-recognized	432:446	well-recognized	432:446	The chemical composition of the mucilage is well-recognized but its structural organization remains unclear.					
30040844	3	46	theme	chemical	392:399	arg1	composition					401:411	The chemical composition	388:411	The chemical composition of the mucilage	388:427	The chemical composition of the mucilage is well-recognized but its structural organization remains unclear.					
30040844	10	47	dep	architecture	1518:1529	arg1	The					1508:1510	The	1508:1510	The	1508:1510	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	8	48	theme	Cellulose	1233:1241	arg1	fibrils					1243:1249	Cellulose fibrils	1233:1249	Cellulose fibrils	1233:1249	Cellulose fibrils built a kind of scaffold for the rest of the components which were spread between them and/or covered their surface.					
30040844	6	49	theme	ordered	986:992	arg1	arrangement					994:1004	a more ordered arrangement	979:1004	a more ordered arrangement in Ocimum basilicum	979:1024	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	0	50	theme	spatial	25:31	arg1	architecture					33:44	The micro- and nanoscale spatial architecture	0:44	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species	0:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	7	51	theme	fibrillary	1070:1079	arg1	components					1081:1090	the mucilaginous fibrillary components	1053:1090	the mucilaginous fibrillary components	1053:1090	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	10	52	theme	loose	1512:1516	arg1	architecture					1518:1529	loose architecture	1512:1529	loose architecture	1512:1529	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	1	53	dep	gel-like	203:210	arg1	transparent					213:223	transparent	213:223	transparent	213:223	The seed coat mucilage envelope is formed just after hydration and surrounds the seed as a gel-like, transparent capsule.					
30040844	5	54	theme	down	794:797	arg1	micro-					787:792	the micro-	783:792	the micro- down to the nanoscale	783:814	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	7	55	theme	cellulose	1141:1149	arg1	fibrils					1151:1157	long, unbranched, thicker cellulose fibrils	1115:1157	long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides	1115:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	5	56	theme	mucilage	769:776	arg1	details					754:760	the structural details	739:760	the structural details of the mucilage from the micro- down to the nanoscale	739:814	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	6	57	theme	studied	901:907	arg1	taxa					909:912	the studied taxa	897:912	the studied taxa	897:912	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	7	58	theme	thinner	1182:1188	arg1	polysaccharides					1216:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	6	59	theme	spatial	856:862	arg1	structure					864:872	a visibly spatial structure	846:872	a visibly spatial structure which differed between the studied taxa	846:912	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	9	60	theme	envelope	1472:1479	arg1	loss					1451:1454	the loss	1447:1454	the loss of the mucilage envelope	1447:1479	The cellulose fibrils were attached to the seed surface, and therefore prevent the loss of the mucilage envelope during mechanical impacts.					
30040844	6	61	theme	tangled	930:936	arg1	organization					938:949	a tangled organization	928:949	a tangled organization in Arabidopsis thaliana	928:973	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30040844	1	62	theme	seed	116:119	arg1	envelope					135:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope is formed just after hydration and surrounds the seed as a gel-like, transparent capsule.					
30040844	10	63	theme	envelope	1704:1711	arg1	proper					1664:1669	proper	1664:1669	proper	1664:1669	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	7	64	theme	long	1115:1118	arg1	fibrils					1151:1157	long, unbranched, thicker cellulose fibrils	1115:1157	long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides	1115:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	10	65	theme	cell	1584:1587	arg1	wall					1589:1592	the mucilaginous cell wall	1567:1592	the mucilaginous cell wall	1567:1592	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	10	66	theme	mucilage	1695:1702	arg1	envelope					1704:1711	the seed mucilage envelope	1686:1711	the seed mucilage envelope	1686:1711	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	1	67	theme	coat	121:124	arg1	envelope					135:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope is formed just after hydration and surrounds the seed as a gel-like, transparent capsule.					
30040844	1	68	theme	gel-like	203:210	arg1	capsule					225:231	a gel-like, transparent capsule	201:231	a gel-like, transparent capsule	201:231	The seed coat mucilage envelope is formed just after hydration and surrounds the seed as a gel-like, transparent capsule.					
30040844	5	69	theme	scanning	679:686	arg1	microscopy					697:706	scanning electron microscopy	679:706	scanning electron microscopy (SEM)	679:712	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	5	69	theme	scanning	679:686	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Using critical point drying (CPD) and scanning electron microscopy (SEM) imaging, we demonstrated the structural details of the mucilage from the micro- down to the nanoscale.					
30040844	2	70	theme	mucilage	238:245	arg1	envelope					247:254	The mucilage envelope	234:254	The mucilage envelope	234:254	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	1	71	theme	mucilage	126:133	arg1	envelope					135:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope	112:142	The seed coat mucilage envelope is formed just after hydration and surrounds the seed as a gel-like, transparent capsule.					
30040844	4	72	theme	selected	593:600	arg1	taxa					602:605	selected taxa	593:605	selected taxa which produce cellulose mucilage	593:638	In the presented study, we visualized the spatial architecture of the seed mucilage envelope of selected taxa which produce cellulose mucilage.					
30040844	0	73	theme	mucilage-Comparative	58:77	arg1	study					79:83	the seed mucilage-Comparative study	49:83	the seed mucilage-Comparative study of selected plant species	49:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	10	74	theme	water	1611:1615	arg1	binding					1617:1623	water binding	1611:1623	water binding	1611:1623	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	8	75	theme	scaffold	1267:1274	arg1	kind					1259:1262	a kind	1257:1262	a kind of scaffold for the rest of the components which were spread between them and/or covered their surface	1257:1365	Cellulose fibrils built a kind of scaffold for the rest of the components which were spread between them and/or covered their surface.					
30040844	0	76	theme	seed	53:56	arg1	study					79:83	the seed mucilage-Comparative study	49:83	the seed mucilage-Comparative study of selected plant species	49:109	The micro- and nanoscale spatial architecture of the seed mucilage-Comparative study of selected plant species.					
30040844	7	77	theme	other	1210:1214	arg1	polysaccharides					1216:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	shorter, thinner and, often branched other polysaccharides	1173:1230	In general, the mucilaginous fibrillary components formed network made of long, unbranched, thicker cellulose fibrils together with shorter, thinner and, often branched other polysaccharides.					
30040844	2	78	theme	i.e.	344:347	arg1	cellulose					349:357	i.e. cellulose	344:357	the typical polysaccharides i.e. cellulose	316:357	The mucilage envelope represents a special type of modified cell wall with all of the typical polysaccharides i.e. cellulose, pectins and hemicelluloses.					
30040844	10	79	theme	mucilaginous	1571:1582	arg1	wall					1589:1592	the mucilaginous cell wall	1567:1592	the mucilaginous cell wall	1567:1592	The loose architecture and special chemical composition of the mucilaginous cell wall is important for water binding and storage, which are crucial for the proper functioning of the seed mucilage envelope.					
30040844	6	80	theme	Arabidopsis	954:964	arg1	thaliana					966:973	Arabidopsis thaliana	954:973	Arabidopsis thaliana	954:973	The mucilage, after CPD, had a visibly spatial structure which differed between the studied taxa; for example, a tangled organization in Arabidopsis thaliana and a more ordered arrangement in Ocimum basilicum were revealed.					
30849468	0	0	theme	Chimonobambusa	83:96	arg1	by-products					125:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	3	1	theme	molecular	689:697	arg1	weights					699:705	molecular weights	689:705	molecular weights	689:705	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	6	2	theme	composition	1243:1253	arg1	weight					1199:1204	smaller molecular weight	1181:1204	smaller molecular weight	1181:1204	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	6	2	theme	composition	1243:1253	arg1	content					1217:1223	lower content	1211:1223	lower content of monosaccharide composition of glucose for the CPS-UAE	1211:1280	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	6	2	theme	composition	1243:1253	arg1	content					1172:1178	the higher uronic acid content	1149:1178	the higher uronic acid content	1149:1178	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	5	3	dep	lowest	1015:1020	arg1	value					1051:1055	medium-high-molecular weight value	1022:1055	the lowest medium-high-molecular weight value (117.49 kDa)	1011:1068	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	3	dep	lowest	1015:1020	arg1	117.49 kDa					1058:1067	117.49 kDa	1058:1067	117.49 kDa	1058:1067	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	4	4	theme	medium-high-molecular-weight	882:909	arg1	value					911:915	the highest medium-high-molecular-weight value	870:915	the highest medium-high-molecular-weight value (136.07 kDa)	870:928	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	4	theme	medium-high-molecular-weight	882:909	arg1	136.07 kDa					918:927	136.07 kDa	918:927	136.07 kDa	918:927	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	3	5	theme	monosaccharide	664:677	arg1	contents					679:686	monosaccharide contents	664:686	monosaccharide contents	664:686	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	6	6	theme	monosaccharide	1228:1241	arg1	composition					1243:1253	monosaccharide composition	1228:1253	monosaccharide composition of glucose	1228:1264	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	5	7	theme	medium-high-molecular	1022:1042	arg1	value					1051:1055	medium-high-molecular weight value	1022:1055	the lowest medium-high-molecular weight value (117.49 kDa)	1011:1068	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	7	theme	medium-high-molecular	1022:1042	arg1	117.49 kDa					1058:1067	117.49 kDa	1058:1067	117.49 kDa	1058:1067	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	1	8	theme	antioxidant	250:260	arg1	activity					262:269	antioxidant activity	250:269	antioxidant activity	250:269	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	6	9	theme	uronic	1160:1165	arg1	content					1172:1178	the higher uronic acid content	1149:1178	the higher uronic acid content	1149:1178	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	4	10	theme	notable	934:940	arg1	ability					954:960	notable antioxidant ability	934:960	notable antioxidant ability	934:960	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	0	11	theme	quadrangularis	98:111	arg1	by-products					125:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	3	12	theme	antioxidant	711:721	arg1	activities					723:732	antioxidant activities	711:732	antioxidant activities	711:732	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	2	13	theme	enzyme-assisted	567:581	arg1	EAE					595:597	EAE	595:597	EAE	595:597	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	13	theme	enzyme-assisted	567:581	arg1	extraction					583:592	enzyme-assisted extraction	567:592	enzyme-assisted extraction (EAE)	567:598	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	7	14	theme	promising	1390:1398	arg1	way					1416:1418	a promising and alternative way	1388:1418	a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization	1388:1519	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	7	14	theme	promising	1390:1398	arg1	technique					1369:1377	ASE technique	1365:1377	ASE technique	1365:1377	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	5	15	contain	had	971:973	arg1	UAE-CPS					963:969	UAE-CPS	963:969	UAE-CPS	963:969	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	15	contain	had	971:973	arg2	highest					979:985	highest	979:985	highest	979:985	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	15	contain	had	971:973	arg2	lowest					1015:1020	lowest	1015:1020	lowest	1015:1020	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	7	16	theme	high	1446:1449	arg1	yield					1451:1455	its high yield	1442:1455	its high yield	1442:1455	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	4	17	theme	antioxidant	942:952	arg1	ability					954:960	notable antioxidant ability	934:960	notable antioxidant ability	934:960	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	6	18	theme	glucose	1258:1264	arg1	composition					1243:1253	monosaccharide composition	1228:1253	monosaccharide composition of glucose	1228:1264	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	6	19	theme	lower	1211:1215	arg1	content					1217:1223	lower content	1211:1223	lower content of monosaccharide composition of glucose for the CPS-UAE	1211:1280	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	2	20	theme	hot	423:425	arg1	HWE					445:447	HWE	445:447	HWE	445:447	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	20	theme	hot	423:425	arg1	extraction					433:442	hot water extraction	423:442	hot water extraction (HWE)	423:448	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	6	21	theme	correlation	1127:1137	arg1	analysis					1139:1146	the correlation analysis	1123:1146	the correlation analysis	1123:1146	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	0	22	theme	processing	114:123	arg1	by-products					125:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	5	23	theme	antioxidant	1079:1089	arg1	activity					1091:1098	its antioxidant activity	1075:1098	its antioxidant activity	1075:1098	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	23	theme	antioxidant	1079:1089	arg1	best					1108:1111	best	1108:1111	best	1108:1111	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	6	24	theme	higher	1306:1311	arg1	activity					1325:1332	its higher antioxidant activity	1302:1332	its higher antioxidant activity	1302:1332	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	7	25	theme	alternative	1404:1414	arg1	way					1416:1418	a promising and alternative way	1388:1418	a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization	1388:1519	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	7	25	theme	alternative	1404:1414	arg1	technique					1369:1377	ASE technique	1365:1377	ASE technique	1365:1377	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	4	26	theme	extraction	844:853	arg1	yield					855:859	the highest extraction yield	832:859	the highest extraction yield (9.94%)	832:867	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	26	theme	extraction	844:853	arg1	%					866:866	9.94%	862:866	9.94%	862:866	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	5	27	theme	uronic	987:992	arg1	acid					994:997	uronic acid	987:997	the highest uronic acid (9.42%)	975:1005	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	27	theme	uronic	987:992	arg1	%					1004:1004	9.42%	1000:1004	9.42%	1000:1004	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	7	28	theme	ASE	1365:1367	arg1	way					1416:1418	a promising and alternative way	1388:1418	a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization	1388:1519	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	7	28	theme	ASE	1365:1367	arg1	technique					1369:1377	ASE technique	1365:1377	ASE technique	1365:1377	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	6	29	theme	higher	1153:1158	arg1	content					1172:1178	the higher uronic acid content	1149:1178	the higher uronic acid content	1149:1178	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	1	30	theme	polysaccharides	274:288	arg1	yield					220:224	the yield	216:224	the yield	216:224	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	30	theme	polysaccharides	274:288	arg1	structure					236:244	chemical structure	227:244	chemical structure	227:244	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	30	theme	polysaccharides	274:288	arg1	activity					262:269	antioxidant activity	250:269	antioxidant activity	250:269	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	4	31	theme	highest	836:842	arg1	yield					855:859	the highest extraction yield	832:859	the highest extraction yield (9.94%)	832:867	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	31	theme	highest	836:842	arg1	%					866:866	9.94%	862:866	9.94%	862:866	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	0	32	theme	extraction	24:33	arg1	methods					35:41	different extraction methods	14:41	different extraction methods	14:41	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	3	33	theme	CPSs	746:749	arg1	different					770:778	different	770:778	different	770:778	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	33	theme	CPSs	746:749	arg1	contents					654:661	the uronic acid contents	638:661	the uronic acid contents	638:661	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	33	theme	CPSs	746:749	arg1	contents					679:686	monosaccharide contents	664:686	monosaccharide contents	664:686	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	33	theme	CPSs	746:749	arg1	weights					699:705	molecular weights	689:705	molecular weights	689:705	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	33	theme	CPSs	746:749	arg1	activities					723:732	antioxidant activities	711:732	antioxidant activities	711:732	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	1	34	from	activity	262:269	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	34	from	activity	262:269	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	7	35	theme	convenient	1492:1501	arg1	industrialization					1503:1519	convenient industrialization	1492:1519	convenient industrialization	1492:1519	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	1	36	theme	extraction	194:203	arg1	methods					205:211	extraction methods	194:211	extraction methods	194:211	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	0	37	theme	different	14:22	arg1	methods					35:41	different extraction methods	14:41	different extraction methods	14:41	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	1	38	theme	bamboo	295:300	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	38	theme	bamboo	295:300	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	2	39	theme	microwave-assisted	527:544	arg1	MAE					558:560	MAE	558:560	MAE	558:560	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	39	theme	microwave-assisted	527:544	arg1	extraction					546:555	microwave-assisted extraction	527:555	microwave-assisted extraction (MAE)	527:561	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	5	40	theme	weight	1044:1049	arg1	value					1051:1055	medium-high-molecular weight value	1022:1055	the lowest medium-high-molecular weight value (117.49 kDa)	1011:1068	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	40	theme	weight	1044:1049	arg1	117.49 kDa					1058:1067	117.49 kDa	1058:1067	117.49 kDa	1058:1067	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	1	41	theme	methods	205:211	arg1	influences					180:189	the influences	176:189	the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	176:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	42	theme	shoots	302:307	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	42	theme	shoots	302:307	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	6	43	theme	molecular	1189:1197	arg1	weight					1199:1204	smaller molecular weight	1181:1204	smaller molecular weight	1181:1204	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	0	44	theme	methods	35:41	arg1	Comparison					0:9	Comparison	0:9	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.	0:136	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	7	45	theme	extract	1423:1429	arg1	CPS					1431:1433	extract CPS	1423:1433	extract CPS	1423:1433	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	2	46	theme	solvent	463:469	arg1	ASE					483:485	ASE	483:485	ASE	483:485	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	46	theme	solvent	463:469	arg1	extraction					471:480	accelerated solvent extraction	451:480	accelerated solvent extraction (ASE)	451:486	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	7	47	theme	notable	1458:1464	arg1	activity					1478:1485	notable antioxidant activity	1458:1485	notable antioxidant activity	1458:1485	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	6	48	theme	smaller	1181:1187	arg1	weight					1199:1204	smaller molecular weight	1181:1204	smaller molecular weight	1181:1204	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	2	49	theme	ultrasonic-assisted	489:507	arg1	extraction					509:518	ultrasonic-assisted extraction	489:518	ultrasonic-assisted extraction (UAE)	489:524	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	49	theme	ultrasonic-assisted	489:507	arg1	UAE					521:523	UAE	521:523	UAE	521:523	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	50	theme	accelerated	451:461	arg1	ASE					483:485	ASE	483:485	ASE	483:485	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	50	theme	accelerated	451:461	arg1	extraction					471:480	accelerated solvent extraction	451:480	accelerated solvent extraction (ASE)	451:486	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	1	51	theme	Chimonobambusa	310:323	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	51	theme	Chimonobambusa	310:323	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	7	52	theme	antioxidant	1466:1476	arg1	activity					1478:1485	notable antioxidant activity	1458:1485	notable antioxidant activity	1458:1485	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	4	53	theme	highest	874:880	arg1	value					911:915	the highest medium-high-molecular-weight value	870:915	the highest medium-high-molecular-weight value (136.07 kDa)	870:928	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	53	theme	highest	874:880	arg1	136.07 kDa					918:927	136.07 kDa	918:927	136.07 kDa	918:927	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	1	54	from	by-products	352:362	arg1	yield					220:224	the yield	216:224	the yield	216:224	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	54	from	by-products	352:362	arg1	structure					236:244	chemical structure	227:244	chemical structure	227:244	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	54	from	by-products	352:362	arg1	activity					262:269	antioxidant activity	250:269	antioxidant activity	250:269	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	54	from	by-products	352:362	arg1	polysaccharides					274:288	polysaccharides	274:288	polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	274:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	55	theme	quadrangularis	325:338	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	55	theme	quadrangularis	325:338	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	56	from	structure	236:244	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	56	from	structure	236:244	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	0	57	from	by-products	125:135	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products	47:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	7	58	from	viewpoint	1354:1362	arg1	way					1416:1418	a promising and alternative way	1388:1418	a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization	1388:1519	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	7	58	from	viewpoint	1354:1362	arg1	technique					1369:1377	ASE technique	1365:1377	ASE technique	1365:1377	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	1	59	from	yield	220:224	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	59	from	yield	220:224	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	6	60	theme	acid	1167:1170	arg1	content					1172:1178	the higher uronic acid content	1149:1178	the higher uronic acid content	1149:1178	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
30849468	7	61	theme	industrial	1343:1352	arg1	viewpoint					1354:1362	an industrial viewpoint	1340:1362	an industrial viewpoint	1340:1362	From an industrial viewpoint, ASE technique could be a promising and alternative way to extract CPS due to its high yield, notable antioxidant activity, and convenient industrialization.					
30849468	5	62	dep	highest	979:985	arg1	acid					994:997	uronic acid	987:997	the highest uronic acid (9.42%)	975:1005	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	5	62	dep	highest	979:985	arg1	%					1004:1004	9.42%	1000:1004	9.42%	1000:1004	UAE-CPS had the highest uronic acid (9.42%) and the lowest medium-high-molecular weight value (117.49 kDa), and its antioxidant activity was the best.					
30849468	3	63	theme	uronic	642:647	arg1	different					770:778	different	770:778	different	770:778	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	63	theme	uronic	642:647	arg1	contents					654:661	the uronic acid contents	638:661	the uronic acid contents	638:661	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	64	theme	experimental	605:616	arg1	results					618:624	The experimental results	601:624	The experimental results	601:624	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	1	65	from	influences	180:189	arg1	yield					220:224	the yield	216:224	the yield	216:224	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	65	from	influences	180:189	arg1	structure					236:244	chemical structure	227:244	chemical structure	227:244	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	65	from	influences	180:189	arg1	activity					262:269	antioxidant activity	250:269	antioxidant activity	250:269	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	4	66	contain	possessed	822:830	arg2	yield					855:859	the highest extraction yield	832:859	the highest extraction yield (9.94%)	832:867	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	66	contain	possessed	822:830	arg2	%					866:866	9.94%	862:866	9.94%	862:866	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	66	contain	possessed	822:830	arg2	value					911:915	the highest medium-high-molecular-weight value	870:915	the highest medium-high-molecular-weight value (136.07 kDa)	870:928	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	66	contain	possessed	822:830	arg2	136.07 kDa					918:927	136.07 kDa	918:927	136.07 kDa	918:927	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	66	contain	possessed	822:830	arg2	ability					954:960	notable antioxidant ability	934:960	notable antioxidant ability	934:960	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	66	contain	possessed	822:830	arg1	CPS					781:783	CPS	781:783	CPS extracted using ASE method (ASE-CPS)	781:820	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	0	67	theme	shoots	75:80	arg1	by-products					125:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	3	68	theme	acid	649:652	arg1	different					770:778	different	770:778	different	770:778	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	3	68	theme	acid	649:652	arg1	contents					654:661	the uronic acid contents	638:661	the uronic acid contents	638:661	The experimental results showed that the uronic acid contents, monosaccharide contents, molecular weights and antioxidant activities of the five CPSs were significantly different.					
30849468	4	69	theme	ASE	801:803	arg1	ASE-CPS					813:819	ASE-CPS	813:819	ASE-CPS	813:819	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	4	69	theme	ASE	801:803	arg1	method					805:810	ASE method	801:810	ASE method (ASE-CPS)	801:820	CPS extracted using ASE method (ASE-CPS) possessed the highest extraction yield (9.94%), the highest medium-high-molecular-weight value (136.07 kDa) and notable antioxidant ability.					
30849468	2	70	theme	water	427:431	arg1	HWE					445:447	HWE	445:447	HWE	445:447	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	2	70	theme	water	427:431	arg1	extraction					433:442	hot water extraction	423:442	hot water extraction (HWE)	423:448	CPSs were extracted by using five methods including hot water extraction (HWE), accelerated solvent extraction (ASE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE).					
30849468	1	71	theme	chemical	227:234	arg1	structure					236:244	chemical structure	227:244	chemical structure	227:244	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	0	72	theme	bamboo	68:73	arg1	by-products					125:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	68:135	Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products.					
30849468	1	73	theme	processing	341:350	arg1	CPS					365:367	CPS	365:367	CPS	365:367	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	73	theme	processing	341:350	arg1	by-products					352:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products	295:362	bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS)	295:368	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	1	74	theme	study	154:158	arg1	aim					142:144	The aim	138:144	The aim of this study	138:158	The aim of this study was to evaluate the influences of extraction methods on the yield, chemical structure and antioxidant activity of polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products (CPS).					
30849468	6	75	theme	antioxidant	1313:1323	arg1	activity					1325:1332	its higher antioxidant activity	1302:1332	its higher antioxidant activity	1302:1332	Based on the correlation analysis, the higher uronic acid content, smaller molecular weight, and lower content of monosaccharide composition of glucose for the CPS-UAE might contribute to its higher antioxidant activity.					
31739015	3	0	theme	chromatographies	507:522	arg1	combination					460:470	a combination	458:470	a combination of ion-exchange and gel permeation chromatographies	458:522	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	4	1	theme	HPLC	604:607	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	1	theme	HPLC	604:607	arg1	ability					681:687	anti-hemolysis inhibitory ability	655:687	anti-hemolysis inhibitory ability	655:687	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	1	theme	HPLC	604:607	arg1	effect					701:706	protective effect	690:706	protective effect against DNA damage	690:725	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	0	2	theme	japonica	92:99	arg1	Thunb					101:105	Lonicera japonica Thunb	83:105	Lonicera japonica Thunb	83:105	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	1	3	theme	due	163:165	arg1	food					137:140	food	137:140	food	137:140	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	1	3	theme	due	163:165	arg1	japonica					111:118	L. japonica	108:118	L. japonica	108:118	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	8	4	theme	evaluated	1353:1361	arg1	methods					1363:1369	evaluated methods	1353:1369	evaluated methods comparable with others	1353:1392	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	7	5	theme	RG-I	1167:1170	arg1	domain					1172:1177	RG-I domain	1167:1177	RG-I domain	1167:1177	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	6	6	with	GalA	1001:1004	arg1	residues					1035:1042	some Gal and Ara residues	1018:1042	residues	1035:1042	LJP-A-2 ~ LJP-A-4 were similar to each other, mainly composed of GalA (>50%) with some Gal and Ara residues, while LJP-A-1 mainly composed of Gal and Ara (>70%).					
31739015	5	7	dep	383.8 kDa	925:933	arg1	to					922:923	to	922:923	to	922:923	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	4	8	theme	FT-IR	610:614	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	8	theme	FT-IR	610:614	arg1	ability					681:687	anti-hemolysis inhibitory ability	655:687	anti-hemolysis inhibitory ability	655:687	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	8	theme	FT-IR	610:614	arg1	effect					701:706	protective effect	690:706	protective effect against DNA damage	690:725	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	7	9	theme	domain	1172:1177	arg1	trace					1158:1162	a trace	1156:1162	a trace of RG-I domain by NMR experiment	1156:1195	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	8	10	theme	antioxidant	1314:1324	arg1	activities					1326:1335	noticeable antioxidant activities	1303:1335	noticeable antioxidant activities	1303:1335	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	8	11	theme	noticeable	1303:1312	arg1	activities					1326:1335	noticeable antioxidant activities	1303:1335	noticeable antioxidant activities	1303:1335	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	5	12	theme	starch-like	760:770	arg1	glucan					772:777	a starch-like glucan	758:777	a starch-like glucan with some arabinogalactan	758:803	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	5	12	theme	starch-like	760:770	arg1	LJP-N					748:752	LJP-N	748:752	LJP-N	748:752	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	9	13	theme	potential	1461:1469	arg1	source					1479:1484	a potential natural source	1459:1484	a potential natural source of antioxidant	1459:1499	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	13	theme	potential	1461:1469	arg1	LJP-A-4					1434:1440	LJP-A-4	1434:1440	LJP-A-4	1434:1440	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	13	theme	potential	1461:1469	arg1	LJP-A-3					1422:1428	LJP-A-3	1422:1428	LJP-A-3	1422:1428	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	8	14	theme	LJP	1226:1228	arg1	fractions					1230:1238	LJP fractions	1226:1238	LJP fractions	1226:1238	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	8	15	theme	antioxidant	1202:1212	arg1	activity					1214:1221	The antioxidant activity	1198:1221	The antioxidant activity of LJP fractions	1198:1238	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	7	16	theme	HG-type	1121:1127	arg1	LJP-A-3					1098:1104	LJP-A-3	1098:1104	LJP-A-3	1098:1104	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	7	16	theme	HG-type	1121:1127	arg1	polysacchride					1136:1148	HG-type pectic polysacchride	1121:1148	HG-type pectic polysacchride	1121:1148	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	9	17	theme	natural	1471:1477	arg1	source					1479:1484	a potential natural source	1459:1484	a potential natural source of antioxidant	1459:1499	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	17	theme	natural	1471:1477	arg1	LJP-A-4					1434:1440	LJP-A-4	1434:1440	LJP-A-4	1434:1440	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	17	theme	natural	1471:1477	arg1	LJP-A-3					1422:1428	LJP-A-3	1422:1428	LJP-A-3	1422:1428	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	18	used	used	1451:1454	arg2	LJP-A-3					1422:1428	LJP-A-3	1422:1428	LJP-A-3	1422:1428	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	18	used	used	1451:1454	arg2	source					1479:1484	a potential natural source	1459:1484	a potential natural source of antioxidant	1459:1499	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	18	used	used	1451:1454	arg2	LJP-A-4					1434:1440	LJP-A-4	1434:1440	LJP-A-4	1434:1440	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	5	19	theme	pectic	832:837	arg1	polysaccharide					839:852	all pectic polysaccharide	828:852	all pectic polysaccharide	828:852	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	7	20	theme	NMR	1182:1184	arg1	experiment					1186:1195	NMR experiment	1182:1195	NMR experiment	1182:1195	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	1	21	theme	good	174:177	arg1	nutrition					179:187	the good nutrition	170:187	the good nutrition	170:187	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	4	22	theme	factions	576:583	arg1	activities					556:565	antioxidant activities	544:565	antioxidant activities	544:565	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	22	theme	factions	576:583	arg1	structures					529:538	structures	529:538	structures	529:538	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	6	23	dep	composed	1066:1073	arg1	LJP-A-1					1051:1057	LJP-A-1	1051:1057	LJP-A-1 mainly composed of Gal and Ara (>70%)	1051:1095	LJP-A-2 ~ LJP-A-4 were similar to each other, mainly composed of GalA (>50%) with some Gal and Ara residues, while LJP-A-1 mainly composed of Gal and Ara (>70%).					
31739015	6	23	dep	composed	1066:1073	arg1	%					1094:1094	>70%	1091:1094	>70%	1091:1094	LJP-A-2 ~ LJP-A-4 were similar to each other, mainly composed of GalA (>50%) with some Gal and Ara residues, while LJP-A-1 mainly composed of Gal and Ara (>70%).					
31739015	4	24	theme	protective	690:699	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	24	theme	protective	690:699	arg1	effect					701:706	protective effect	690:706	protective effect against DNA damage	690:725	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	3	25	theme	neutral	384:390	arg1	LJP-N					402:406	LJP-N	402:406	LJP-N	402:406	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	3	25	theme	neutral	384:390	arg1	fraction					392:399	one neutral fraction	380:399	one neutral fraction (LJP-N)	380:407	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	1	26	used	used	129:132	arg2	beverage					154:161	healthy beverage	146:161	healthy beverage	146:161	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	1	26	used	used	129:132	arg2	japonica					111:118	L. japonica	108:118	L. japonica	108:118	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	1	26	used	used	129:132	arg2	food					137:140	food	137:140	food	137:140	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	8	27	theme	fractions	1230:1238	arg1	activity					1214:1221	The antioxidant activity	1198:1221	The antioxidant activity of LJP fractions	1198:1238	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	0	28	theme	antioxidant	18:28	arg1	activities					30:39	antioxidant activities	18:39	antioxidant activities	18:39	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	0	29	from	Thunb	101:105	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	0	29	from	Thunb	101:105	arg1	activities					30:39	antioxidant activities	18:39	antioxidant activities	18:39	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	5	30	with	polysaccharide	839:852	arg1	weights					882:888	the average molecular weights	860:888	the average molecular weights approximately ranging from 19.0 to 383.8 kDa	860:933	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	5	31	theme	average	864:870	arg1	weights					882:888	the average molecular weights	860:888	the average molecular weights approximately ranging from 19.0 to 383.8 kDa	860:933	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	5	32	theme	acidic	806:811	arg1	fractions					813:821	acidic fractions	806:821	acidic fractions	806:821	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	8	33	with	comparable	1371:1380	arg1	others					1387:1392	others	1387:1392	others	1387:1392	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	5	34	theme	molecular	872:880	arg1	weights					882:888	the average molecular weights	860:888	the average molecular weights approximately ranging from 19.0 to 383.8 kDa	860:933	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	4	35	theme	inhibitory	670:679	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	35	theme	inhibitory	670:679	arg1	ability					681:687	anti-hemolysis inhibitory ability	655:687	anti-hemolysis inhibitory ability	655:687	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	3	36	theme	acidic	418:423	arg1	LJP-A-1 ~ LJP-A-4					436:452	LJP-A-1 ~ LJP-A-4	436:452	LJP-A-1 ~ LJP-A-4	436:452	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	3	36	theme	acidic	418:423	arg1	fractions					425:433	four acidic fractions	413:433	four acidic fractions (LJP-A-1 ~ LJP-A-4)	413:453	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	4	37	theme	antioxidant	544:554	arg1	activities					556:565	antioxidant activities	544:565	antioxidant activities	544:565	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	7	38	theme	pectic	1129:1134	arg1	LJP-A-3					1098:1104	LJP-A-3	1098:1104	LJP-A-3	1098:1104	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	7	38	theme	pectic	1129:1134	arg1	polysacchride					1136:1148	HG-type pectic polysacchride	1121:1148	HG-type pectic polysacchride	1121:1148	LJP-A-3 was defined as HG-type pectic polysacchride, with a trace of RG-I domain by NMR experiment.					
31739015	8	39	theme	different	1247:1255	arg1	activities					1257:1266	different activities	1247:1266	different activities	1247:1266	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	5	40	with	glucan	772:777	arg1	arabinogalactan					789:803	some arabinogalactan	784:803	some arabinogalactan	784:803	Results showed that LJP-N was a starch-like glucan with some arabinogalactan; acidic fractions were all pectic polysaccharide, with the average molecular weights approximately ranging from 19.0 to 383.8 kDa.					
31739015	4	41	theme	anti-hemolysis	655:668	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	41	theme	anti-hemolysis	655:668	arg1	ability					681:687	anti-hemolysis inhibitory ability	655:687	anti-hemolysis inhibitory ability	655:687	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	0	42	theme	polysaccharides	62:76	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	0	42	theme	polysaccharides	62:76	arg1	activities					30:39	antioxidant activities	18:39	antioxidant activities	18:39	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	4	43	dep	structures	529:538	arg1	The					525:527	The	525:527	The	525:527	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	3	44	theme	gel	492:494	arg1	chromatographies					507:522	gel permeation chromatographies	492:522	gel permeation chromatographies	492:522	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	0	45	theme	water-soluble	48:60	arg1	polysaccharides					62:76	the water-soluble polysaccharides	44:76	the water-soluble polysaccharides	44:76	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	3	46	theme	water-soluble	312:324	arg1	polysaccharides					326:340	water-soluble polysaccharides	312:340	water-soluble polysaccharides of L. japonica	312:355	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	2	47	theme	chemical	203:210	arg1	compounds					212:220	the chemical compounds	199:220	the chemical compounds	199:220	Although the chemical compounds have been extensively studied, polysaccharide compositions remain unclear.					
31739015	3	48	theme	permeation	496:505	arg1	chromatographies					507:522	gel permeation chromatographies	492:522	gel permeation chromatographies	492:522	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	8	49	theme	comparable	1371:1380	arg1	methods					1363:1369	evaluated methods	1353:1369	evaluated methods comparable with others	1353:1392	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	3	50	theme	ion-exchange	475:486	arg1	combination					460:470	a combination	458:470	a combination of ion-exchange and gel permeation chromatographies	458:522	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	8	51	theme	methods	1363:1369	arg1	kinds					1344:1348	six kinds	1340:1348	six kinds of evaluated methods comparable with others	1340:1392	The antioxidant activity of LJP fractions showed different activities, and LJP-A-3 and LJP-A-4 exhibited noticeable antioxidant activities by six kinds of evaluated methods comparable with others.					
31739015	2	52	theme	polysaccharide	253:266	arg1	compositions					268:279	polysaccharide compositions	253:279	polysaccharide compositions	253:279	Although the chemical compounds have been extensively studied, polysaccharide compositions remain unclear.					
31739015	6	53	theme	Ara	1031:1033	arg1	residues					1035:1042	some Gal and Ara residues	1018:1042	residues	1035:1042	LJP-A-2 ~ LJP-A-4 were similar to each other, mainly composed of GalA (>50%) with some Gal and Ara residues, while LJP-A-1 mainly composed of Gal and Ara (>70%).					
31739015	0	54	theme	Lonicera	83:90	arg1	Thunb					101:105	Lonicera japonica Thunb	83:105	Lonicera japonica Thunb	83:105	Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb.					
31739015	9	55	theme	antioxidant	1489:1499	arg1	source					1479:1484	a potential natural source	1459:1484	a potential natural source of antioxidant	1459:1499	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	55	theme	antioxidant	1489:1499	arg1	LJP-A-4					1434:1440	LJP-A-4	1434:1440	LJP-A-4	1434:1440	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	9	55	theme	antioxidant	1489:1499	arg1	LJP-A-3					1422:1428	LJP-A-3	1422:1428	LJP-A-3	1422:1428	The results indicated that LJP-A-3 and LJP-A-4 could be used as a potential natural source of antioxidant.					
31739015	4	56	theme	DNA	716:718	arg1	damage					720:725	DNA damage	716:725	DNA damage	716:725	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	57	theme	scavenging	632:641	arg1	activities					643:652	HPLC, FT-IR and the radical scavenging activities	604:652	HPLC, FT-IR and the radical scavenging activities	604:652	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	57	theme	scavenging	632:641	arg1	ability					681:687	anti-hemolysis inhibitory ability	655:687	anti-hemolysis inhibitory ability	655:687	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	4	57	theme	scavenging	632:641	arg1	effect					701:706	protective effect	690:706	protective effect against DNA damage	690:725	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31739015	3	58	theme	japonica	348:355	arg1	polysaccharides					326:340	water-soluble polysaccharides	312:340	water-soluble polysaccharides of L. japonica	312:355	In this study, water-soluble polysaccharides of L. japonica were fractionated into one neutral fraction (LJP-N) and four acidic fractions (LJP-A-1 ~ LJP-A-4) by a combination of ion-exchange and gel permeation chromatographies.					
31739015	6	59	theme	Gal	1023:1025	arg1	residues					1035:1042	some Gal and Ara residues	1018:1042	residues	1035:1042	LJP-A-2 ~ LJP-A-4 were similar to each other, mainly composed of GalA (>50%) with some Gal and Ara residues, while LJP-A-1 mainly composed of Gal and Ara (>70%).					
31739015	1	60	theme	healthy	146:152	arg1	japonica					111:118	L. japonica	108:118	L. japonica	108:118	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	1	60	theme	healthy	146:152	arg1	beverage					154:161	healthy beverage	146:161	healthy beverage	146:161	L. japonica has been used as food and healthy beverage due to the good nutrition.					
31739015	4	61	theme	radical	624:630	arg1	scavenging					632:641	the radical scavenging	620:641	the radical scavenging	620:641	The structures and antioxidant activities of these factions were determined by HPLC, FT-IR and the radical scavenging activities, anti-hemolysis inhibitory ability, protective effect against DNA damage.					
31359698	6	0	from	effects	946:952	arg1	cells					988:992	human breast cancer MDA-MB-231 cells	957:992	human breast cancer MDA-MB-231 cells	957:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	0	1	from	effect	62:67	arg1	migration					100:108	migration	100:108	migration of human breast cancer MDA-MB-231 cells	100:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	1	from	effect	62:67	arg1	cocos					52:56	Poria cocos	46:56	Poria cocos	46:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	11	2	attach	isolated	1625:1632	arg2	kind					1523:1526	a kind	1521:1526	a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells	1521:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	11	2	attach	isolated	1625:1632	arg1	cocos					1642:1646	P. cocos	1639:1646	P. cocos	1639:1646	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	7	3	theme	P.	1126:1127	arg1	cocos					1129:1133	P. cocos	1126:1133	P. cocos	1126:1133	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	11	4	theme	polysaccharide	1543:1556	arg1	kind					1523:1526	a kind	1521:1526	a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells	1521:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	0	5	theme	cells	144:148	arg1	migration					100:108	migration	100:108	migration of human breast cancer MDA-MB-231 cells	100:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	3	6	theme	ethanol	576:582	arg1	extraction					584:593	ethanol extraction	576:593	ethanol extraction	576:593	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	8	7	theme	1,6-branched	1198:1209	arg1	1,3-α-D-galactan					1211:1226	1,6-branched 1,3-α-D-galactan	1198:1226	1,6-branched 1,3-α-D-galactan	1198:1226	06×104,and structure of 1,6-branched 1,3-α-D-galactan.					
31359698	11	8	theme	substance	1763:1771	arg1	study					1779:1783	the substance basis study	1759:1783	the substance basis study of P. cocos	1759:1795	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	0	9	theme	human	113:117	arg1	cells					144:148	human breast cancer MDA-MB-231 cells	113:148	human breast cancer MDA-MB-231 cells	113:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	5	10	theme	PPSW-1	858:863	arg1	structure					845:853	The structure	841:853	The structure of PPSW-1	841:863	The structure of PPSW-1 was identified and its sulfated derivatives were prepared.					
31359698	0	11	theme	cancer	126:131	arg1	cells					144:148	human breast cancer MDA-MB-231 cells	113:148	human breast cancer MDA-MB-231 cells	113:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	9	12	theme	derivative	1253:1262	arg1	Sul-W-1					1264:1270	its sulfated derivative Sul-W-1	1240:1270	its sulfated derivative Sul-W-1	1240:1270	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	0	13	theme	polysaccharide	26:39	arg1	[Isolation					0:9	[Isolation	0:9	[Isolation of homogeneous polysaccharide from Poria cocos	0:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	13	theme	polysaccharide	26:39	arg1	effect					62:67	effect	62:67	effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells	62:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	6	14	theme	cancer	970:975	arg1	cells					988:992	human breast cancer MDA-MB-231 cells	957:992	human breast cancer MDA-MB-231 cells	957:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	2	15	from	effects	492:498	arg1	expression					509:518	SATB1 expression	503:518	SATB1 expression	503:518	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	11	16	theme	P.	1788:1789	arg1	cocos					1791:1795	P. cocos	1788:1795	P. cocos	1788:1795	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	7	17	theme	breast	1077:1082	arg1	cells					1102:1106	human breast cancer MDA-MB-231 cells	1071:1106	human breast cancer MDA-MB-231 cells	1071:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	4	18	dep	flow	752:755	arg1	steps					819:823	chromatographic steps	803:823	chromatographic steps	803:823	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	6	19	theme	human	957:961	arg1	cells					988:992	human breast cancer MDA-MB-231 cells	957:992	human breast cancer MDA-MB-231 cells	957:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	9	20	theme	inhibitory	1284:1293	arg1	effect					1295:1300	good inhibitory effect	1279:1300	good inhibitory effect	1279:1300	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	3	21	theme	crude	608:612	arg1	PPS					629:631	P. cocos crude polysaccharide PPS	599:631	P. cocos crude polysaccharide PPS	599:631	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	2	22	with	compositions	463:474	arg1	effects					492:498	inhibitory effects	481:498	inhibitory effects on SATB1 expression	481:518	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	2	23	theme	inhibitory	481:490	arg1	effects					492:498	inhibitory effects	481:498	inhibitory effects on SATB1 expression	481:518	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	10	24	theme	cocos	1440:1444	arg1	polysaccharide					1419:1432	polysaccharide	1419:1432	polysaccharide of P. cocos	1419:1444	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	10	24	theme	cocos	1440:1444	arg1	derivative					1463:1472	its sulfated derivative	1450:1472	its sulfated derivative	1450:1472	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	7	25	with	PPSW-1	1038:1043	arg1	effect					1061:1066	inhibitory effect	1050:1066	inhibitory effect on human breast cancer MDA-MB-231 cells	1050:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	0	26	from	[Isolation	0:9	arg1	migration					100:108	migration	100:108	migration of human breast cancer MDA-MB-231 cells	100:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	26	from	[Isolation	0:9	arg1	cocos					52:56	Poria cocos	46:56	Poria cocos	46:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	4	27	theme	Superdex-75	776:786	arg1	permeation					792:801	Superdex-75 gel permeation	776:801	Superdex-75 gel permeation	776:801	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	0	28	theme	derivatives	85:95	arg1	[Isolation					0:9	[Isolation	0:9	[Isolation of homogeneous polysaccharide from Poria cocos	0:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	28	theme	derivatives	85:95	arg1	effect					62:67	effect	62:67	effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells	62:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	29	from	cocos	52:56	arg1	[Isolation					0:9	[Isolation	0:9	[Isolation of homogeneous polysaccharide from Poria cocos	0:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	29	from	cocos	52:56	arg1	polysaccharide					26:39	homogeneous polysaccharide	14:39	homogeneous polysaccharide from Poria cocos	14:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	0	29	from	cocos	52:56	arg1	effect					62:67	effect	62:67	effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells	62:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	9	30	theme	water	1329:1333	arg1	better					1361:1366	better	1361:1366	better	1361:1366	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	9	30	theme	water	1329:1333	arg1	solubility					1335:1344	the water solubility	1325:1344	the water solubility of Sul-W-1	1325:1355	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	4	31	theme	fast	747:750	arg1	flow					752:755	DEAE Sepharose fast flow anion-exchange	732:770	DEAE Sepharose fast flow anion-exchange	732:770	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	1	32	theme	SATB1	245:249	arg1	expression					251:260	SATB1 expression	245:260	SATB1 expression	245:260	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	11	33	theme	similar	1681:1687	arg1	efficacy					1689:1696	similar efficacy	1681:1696	similar efficacy	1681:1696	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	4	34	theme	anion-exchange	757:770	arg1	flow					752:755	DEAE Sepharose fast flow anion-exchange	732:770	DEAE Sepharose fast flow anion-exchange	732:770	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	0	35	theme	homogeneous	14:24	arg1	polysaccharide					26:39	homogeneous polysaccharide	14:39	homogeneous polysaccharide from Poria cocos	14:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	3	36	theme	water	649:653	arg1	extraction					655:664	water extraction	649:664	water extraction	649:664	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	11	37	theme	human	1584:1588	arg1	cells					1615:1619	human breast cancer MDA-MB-231 cells	1584:1619	human breast cancer MDA-MB-231 cells	1584:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	7	38	theme	molecular	1151:1159	arg1	weight					1161:1166	a relative molecular weight	1140:1166	a relative molecular weight of 3	1140:1171	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	1	39	theme	cancer	293:298	arg1	metastasis					300:309	breast cancer metastasis	286:309	breast cancer metastasis	286:309	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	2	40	attach	isolated	359:366	arg2	polysaccharides					338:352	homogeneous polysaccharides	326:352	homogeneous polysaccharides	326:352	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	2	40	attach	isolated	359:366	arg1	cocos					379:383	Poria cocos	373:383	Poria cocos	373:383	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	1	41	theme	breast	213:218	arg1	cancer					220:225	breast cancer	213:225	breast cancer	213:225	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	11	42	theme	P.	1639:1640	arg1	cocos					1642:1646	P. cocos	1639:1646	P. cocos	1639:1646	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	11	43	theme	cancer	1597:1602	arg1	cells					1615:1619	human breast cancer MDA-MB-231 cells	1584:1619	human breast cancer MDA-MB-231 cells	1584:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	2	44	theme	homogeneous	326:336	arg1	polysaccharides					338:352	homogeneous polysaccharides	326:352	homogeneous polysaccharides	326:352	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	6	45	theme	inhibitory	935:944	arg1	effects					946:952	their inhibitory effects	929:952	their inhibitory effects on human breast cancer MDA-MB-231 cells	929:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	1	46	theme	crucial	166:172	arg1	role					174:177	a crucial role	164:177	a crucial role	164:177	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	5	47	theme	sulfated	888:895	arg1	derivatives					897:907	its sulfated derivatives	884:907	its sulfated derivatives	884:907	The structure of PPSW-1 was identified and its sulfated derivatives were prepared.					
31359698	9	48	theme	Sul-W-1	1349:1355	arg1	better					1361:1366	better	1361:1366	better	1361:1366	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	9	48	theme	Sul-W-1	1349:1355	arg1	solubility					1335:1344	the water solubility	1325:1344	the water solubility of Sul-W-1	1325:1355	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	11	49	theme	better	1702:1707	arg1	solubility					1709:1718	better solubility	1702:1718	better solubility	1702:1718	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	0	50	theme	breast	119:124	arg1	cells					144:148	human breast cancer MDA-MB-231 cells	113:148	human breast cancer MDA-MB-231 cells	113:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	11	51	theme	homogeneous	1531:1541	arg1	polysaccharide					1543:1556	homogeneous polysaccharide	1531:1556	homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells	1531:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	3	52	theme	P.	564:565	arg1	cocos					567:571	P. cocos	564:571	P. cocos	564:571	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	11	53	with	derivative	1665:1674	arg1	efficacy					1689:1696	similar efficacy	1681:1696	similar efficacy	1681:1696	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	11	53	with	derivative	1665:1674	arg1	solubility					1709:1718	better solubility	1702:1718	better solubility	1702:1718	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	11	54	theme	basis	1773:1777	arg1	study					1779:1783	the substance basis study	1759:1783	the substance basis study of P. cocos	1759:1795	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	7	55	theme	cancer	1084:1089	arg1	cells					1102:1106	human breast cancer MDA-MB-231 cells	1071:1106	human breast cancer MDA-MB-231 cells	1071:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	9	56	theme	sulfated	1244:1251	arg1	Sul-W-1					1264:1270	its sulfated derivative Sul-W-1	1240:1270	its sulfated derivative Sul-W-1	1240:1270	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	8	57	theme	1,3-α-D-galactan	1211:1226	arg1	06×104					1174:1179	06×104	1174:1179	06×104	1174:1179	06×104,and structure of 1,6-branched 1,3-α-D-galactan.					
31359698	8	57	theme	1,3-α-D-galactan	1211:1226	arg1	structure					1185:1193	structure	1185:1193	structure	1185:1193	06×104,and structure of 1,6-branched 1,3-α-D-galactan.					
31359698	6	58	theme	MDA-MB-231	977:986	arg1	cells					988:992	human breast cancer MDA-MB-231 cells	957:992	human breast cancer MDA-MB-231 cells	957:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	7	59	theme	human	1071:1075	arg1	cells					1102:1106	human breast cancer MDA-MB-231 cells	1071:1106	human breast cancer MDA-MB-231 cells	1071:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	7	60	theme	inhibitory	1050:1059	arg1	effect					1061:1066	inhibitory effect	1050:1066	inhibitory effect on human breast cancer MDA-MB-231 cells	1050:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	6	61	theme	breast	963:968	arg1	cells					988:992	human breast cancer MDA-MB-231 cells	957:992	human breast cancer MDA-MB-231 cells	957:992	Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated.					
31359698	11	62	theme	cocos	1791:1795	arg1	study					1779:1783	the substance basis study	1759:1783	the substance basis study of P. cocos	1759:1795	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	9	63	theme	good	1279:1282	arg1	effect					1295:1300	good inhibitory effect	1279:1300	good inhibitory effect	1279:1300	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	3	64	theme	polysaccharide	614:627	arg1	PPS					629:631	P. cocos crude polysaccharide PPS	599:631	P. cocos crude polysaccharide PPS	599:631	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	7	65	theme	PPSW-1	1038:1043	arg1	kind					1015:1018	A kind	1013:1018	A kind	1013:1018	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	3	66	theme	cocos	602:606	arg1	PPS					629:631	P. cocos crude polysaccharide PPS	599:631	P. cocos crude polysaccharide PPS	599:631	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	7	67	theme	polysaccharide	1023:1036	arg1	kind					1015:1018	A kind	1013:1018	A kind	1013:1018	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	7	68	from	effect	1061:1066	arg1	cells					1102:1106	human breast cancer MDA-MB-231 cells	1071:1106	human breast cancer MDA-MB-231 cells	1071:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	0	69	theme	sulfated	76:83	arg1	derivatives					85:95	its sulfated derivatives	72:95	its sulfated derivatives	72:95	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	9	70	theme	cells	1305:1309	arg1	migration					1311:1319	cells migration	1305:1319	cells migration	1305:1319	PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1.					
31359698	10	71	theme	P.	1437:1438	arg1	cocos					1440:1444	P. cocos	1437:1444	P. cocos	1437:1444	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	11	72	from	effect	1574:1579	arg1	cells					1615:1619	human breast cancer MDA-MB-231 cells	1584:1619	human breast cancer MDA-MB-231 cells	1584:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	2	73	theme	SATB1	503:507	arg1	expression					509:518	SATB1 expression	503:518	SATB1 expression	503:518	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	10	74	theme	sulfated	1454:1461	arg1	derivative					1463:1472	its sulfated derivative	1450:1472	its sulfated derivative	1450:1472	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	4	75	theme	gel	788:790	arg1	permeation					792:801	Superdex-75 gel permeation	776:801	Superdex-75 gel permeation	776:801	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	2	76	theme	Poria	373:377	arg1	cocos					379:383	Poria cocos	373:383	Poria cocos	373:383	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	2	77	dep	screen	433:438	arg1	out					440:442	out	440:442	out	440:442	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	0	78	theme	MDA-MB-231	133:142	arg1	cells					144:148	human breast cancer MDA-MB-231 cells	113:148	human breast cancer MDA-MB-231 cells	113:148	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	7	79	theme	3	1171:1171	arg1	weight					1161:1166	a relative molecular weight	1140:1166	a relative molecular weight of 3	1140:1171	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	1	80	theme	expression	251:260	arg1	inhibition					231:240	inhibition	231:240	inhibition of SATB1 expression	231:260	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	3	81	attach	removed	551:557	arg2	components					535:544	Smal-molecule components	521:544	Smal-molecule components	521:544	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	3	81	attach	removed	551:557	arg1	cocos					567:571	P. cocos	564:571	P. cocos	564:571	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	11	82	with	polysaccharide	1543:1556	arg1	effect					1574:1579	inhibitory effect	1563:1579	inhibitory effect on human breast cancer MDA-MB-231 cells	1563:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	2	83	theme	sulfated	395:402	arg1	derivatives					404:414	their sulfated derivatives	389:414	their sulfated derivatives	389:414	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	0	84	theme	Poria	46:50	arg1	cocos					52:56	Poria cocos	46:56	Poria cocos	46:56	[Isolation of homogeneous polysaccharide from Poria cocos and effect of its sulfated derivatives on migration of human breast cancer MDA-MB-231 cells].					
31359698	2	85	theme	polysaccharide	448:461	arg1	compositions					463:474	the polysaccharide compositions	444:474	the polysaccharide compositions with inhibitory effects on SATB1 expression	444:518	In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression.					
31359698	11	86	theme	sulfated	1656:1663	arg1	derivative					1665:1674	its sulfated derivative	1652:1674	its sulfated derivative with similar efficacy but better solubility	1652:1718	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	7	87	theme	relative	1142:1149	arg1	weight					1161:1166	a relative molecular weight	1140:1166	a relative molecular weight of 3	1140:1171	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	1	88	theme	breast	286:291	arg1	metastasis					300:309	breast cancer metastasis	286:309	breast cancer metastasis	286:309	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	7	89	theme	MDA-MB-231	1091:1100	arg1	cells					1102:1106	human breast cancer MDA-MB-231 cells	1071:1106	human breast cancer MDA-MB-231 cells	1071:1106	A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3.					
31359698	3	90	theme	ethanol	670:676	arg1	precipitation					678:690	ethanol precipitation	670:690	ethanol precipitation	670:690	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	11	91	theme	inhibitory	1563:1572	arg1	effect					1574:1579	inhibitory effect	1563:1579	inhibitory effect on human breast cancer MDA-MB-231 cells	1563:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	3	92	theme	P.	599:600	arg1	PPS					629:631	P. cocos crude polysaccharide PPS	599:631	P. cocos crude polysaccharide PPS	599:631	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	10	93	located	found	1408:1412	arg1	addition					1392:1399	addition	1392:1399	addition	1392:1399	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	10	93	located	found	1408:1412	arg2	it					1401:1402	it	1401:1402	it	1401:1402	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	10	94	theme	SATB1	1500:1504	arg1	expression					1486:1495	expression	1486:1495	expression of SATB1	1486:1504	In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1.					
31359698	4	95	theme	chromatographic	803:817	arg1	steps					819:823	chromatographic steps	803:823	chromatographic steps	803:823	Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1.					
31359698	11	96	theme	MDA-MB-231	1604:1613	arg1	cells					1615:1619	human breast cancer MDA-MB-231 cells	1584:1619	human breast cancer MDA-MB-231 cells	1584:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	11	97	theme	breast	1590:1595	arg1	cells					1615:1619	human breast cancer MDA-MB-231 cells	1584:1619	human breast cancer MDA-MB-231 cells	1584:1619	In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.					
31359698	3	98	theme	Smal-molecule	521:533	arg1	components					535:544	Smal-molecule components	521:544	Smal-molecule components	521:544	Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation.					
31359698	1	99	theme	cancer	220:225	arg1	metastasis					199:208	metastasis	199:208	metastasis	199:208	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
31359698	1	99	theme	cancer	220:225	arg1	invasion					186:193	invasion	186:193	invasion	186:193	SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis.					
30465842	7	0	theme	water	1234:1238	arg1	capacity					1248:1255	water holding capacity	1234:1255	water holding capacity	1234:1255	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	7	1	theme	branching	1137:1145	arg1	complicated					1165:1175	complicated	1165:1175	complicated	1165:1175	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	7	1	theme	branching	1137:1145	arg1	degree					1112:1117	the degree	1108:1117	the degree of polysaccharide branching	1108:1145	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	6	2	theme	australasicum	888:900	arg1	mucilage					902:909	A. australasicum mucilage	885:909	A. australasicum mucilage	885:909	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	0	3	dep	J.	127:128	arg1	Sm					130:131	Sm	130:131	J. Sm.	127:132	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	0	4	from	properties	60:69	arg1	fronds					92:97	the fronds	88:97	the fronds of Asplenium australasicum	88:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	7	5	theme	holding	1240:1246	arg1	capacity					1248:1255	water holding capacity	1234:1255	water holding capacity	1234:1255	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	6	6	theme	A.	885:886	arg1	mucilage					902:909	A. australasicum mucilage	885:909	A. australasicum mucilage	885:909	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	0	7	from	Effect	0:5	arg1	properties					60:69	the physicochemical properties	40:69	the physicochemical properties of mucilage from the fronds of Asplenium australasicum	40:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	2	8	theme	Enzyme-assisted	141:155	arg1	EAE					169:171	EAE	169:171	EAE	169:171	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	2	8	theme	Enzyme-assisted	141:155	arg1	extraction					157:166	Enzyme-assisted extraction	141:166	Enzyme-assisted extraction (EAE)	141:172	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	7	9	theme	polysaccharide	1122:1135	arg1	branching					1137:1145	polysaccharide branching	1122:1145	polysaccharide branching	1122:1145	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	2	10	theme	reaction	278:285	arg1	conditions					287:296	relatively mild reaction conditions	262:296	relatively mild reaction conditions	262:296	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	4	11	theme	3.64	603:606	arg1	%					607:607	%	607:607	%	607:607	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	0	12	theme	australasicum	112:124	arg1	fronds					92:97	the fronds	88:97	the fronds of Asplenium australasicum	88:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	2	13	theme	mild	273:276	arg1	conditions					287:296	relatively mild reaction conditions	262:296	relatively mild reaction conditions	262:296	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	0	14	theme	Asplenium	102:110	arg1	australasicum					112:124	Asplenium australasicum	102:124	Asplenium australasicum	102:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	6	15	theme	mucilage	902:909	arg1	profile					874:880	the monosaccharide composition profile	843:880	the monosaccharide composition profile of A. australasicum mucilage	843:909	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	5	16	theme	higher	769:774	arg1	efficiency					787:796	the higher extraction efficiency	765:796	the higher extraction efficiency of polysaccharide	765:814	SEM results showed that enzymes can erode the raw material which contributed to the higher extraction efficiency of polysaccharide.					
30465842	7	17	theme	enzymes	1095:1101	arg1	action					1081:1086	the action	1077:1086	the action of the enzymes	1077:1101	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	4	18	theme	molecular	643:651	arg1	weight					653:658	the average molecular weight	631:658	the average molecular weight	631:658	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	3	19	theme	simultaneous	386:397	arg1	application					399:409	simultaneous application	386:409	simultaneous application	386:409	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	4	20	dep	increased	566:574	arg1	%					621:621	3.64% to 6.04-6.86%	603:621	3.64% to 6.04-6.86%	603:621	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	6	21	dep	modified	834:841	arg1	was					942:944	was	942:944	was a significant increase in glucose and galacturonic acid content	942:1008	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	0	22	theme	extraction	26:35	arg1	Effect					0:5	Effect	0:5	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum	0:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	6	23	theme	acid	997:1000	arg1	content					1002:1008	galacturonic acid content	984:1008	galacturonic acid content	984:1008	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	2	24	contain	possesses	174:182	arg1	EAE					169:171	EAE	169:171	EAE	169:171	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	2	24	contain	possesses	174:182	arg1	extraction					157:166	Enzyme-assisted extraction	141:166	Enzyme-assisted extraction (EAE)	141:172	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	2	24	contain	possesses	174:182	arg2	advantages					188:197	the advantages	184:197	the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions	184:296	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	0	25	theme	enzyme-assisted	10:24	arg1	extraction					26:35	enzyme-assisted extraction	10:35	enzyme-assisted extraction	10:35	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	6	26	theme	galacturonic	984:995	arg1	content					1002:1008	galacturonic acid content	984:1008	galacturonic acid content	984:1008	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	3	27	dep	Asplenium	319:327	arg1	australasicum					329:341	Asplenium australasicum	319:341	Asplenium australasicum	319:341	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	4	28	dep	%	607:607	arg1	to					609:610	to	609:610	to	609:610	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	3	29	theme	enzymatic	419:427	arg1	preparations					429:440	both enzymatic preparations	414:440	both enzymatic preparations	414:440	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	4	30	theme	average	635:641	arg1	weight					653:658	the average molecular weight	631:658	the average molecular weight	631:658	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	3	31	theme	physicochemical	470:484	arg1	properties					486:495	their physicochemical properties	464:495	their physicochemical properties	464:495	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	3	32	theme	preparations	429:440	arg1	glucanase					372:380	glucanase	372:380	glucanase	372:380	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	3	32	theme	preparations	429:440	arg1	application					399:409	simultaneous application	386:409	simultaneous application	386:409	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	3	32	theme	preparations	429:440	arg1	xylanase					362:369	xylanase	362:369	xylanase	362:369	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	5	33	theme	extraction	776:785	arg1	efficiency					787:796	the higher extraction efficiency	765:796	the higher extraction efficiency of polysaccharide	765:814	SEM results showed that enzymes can erode the raw material which contributed to the higher extraction efficiency of polysaccharide.					
30465842	7	34	theme	intrinsic	1210:1218	arg1	viscosity					1220:1228	intrinsic viscosity	1210:1228	intrinsic viscosity	1210:1228	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	3	35	dep	extracted	347:355	arg1	investigated					447:458	investigated	447:458	was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties	343:495	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	6	36	theme	composition	862:872	arg1	profile					874:880	the monosaccharide composition profile	843:880	the monosaccharide composition profile of A. australasicum mucilage	843:909	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	2	37	theme	environmentally	208:222	arg1	friendly					224:231	environmentally friendly	208:231	environmentally friendly	208:231	Enzyme-assisted extraction (EAE) possesses the advantages of being environmentally friendly and easily operated owing to relatively mild reaction conditions.					
30465842	7	38	dep	viscosity	1220:1228	arg1	the					1206:1208	the	1206:1208	the	1206:1208	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	0	39	theme	physicochemical	44:58	arg1	properties					60:69	the physicochemical properties	40:69	the physicochemical properties of mucilage from the fronds of Asplenium australasicum	40:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	4	40	theme	mucilage	557:564	arg1	yield					548:552	the yield	544:552	the yield of mucilage	544:564	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	6	41	theme	monosaccharide	847:860	arg1	profile					874:880	the monosaccharide composition profile	843:880	the monosaccharide composition profile of A. australasicum mucilage	843:909	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	6	42	from	increase	960:967	arg1	glucose					972:978	glucose	972:978	glucose	972:978	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	6	42	from	increase	960:967	arg1	content					1002:1008	galacturonic acid content	984:1008	galacturonic acid content	984:1008	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	6	43	theme	significant	948:958	arg1	increase					960:967	a significant increase	946:967	a significant increase in glucose and galacturonic acid content	946:1008	Furthermore, EAE modified the monosaccharide composition profile of A. australasicum mucilage considerably, especially there was a significant increase in glucose and galacturonic acid content.					
30465842	5	44	theme	SEM	685:687	arg1	results					689:695	SEM results	685:695	SEM results	685:695	SEM results showed that enzymes can erode the raw material which contributed to the higher extraction efficiency of polysaccharide.					
30465842	3	45	from	Asplenium	319:327	arg1	mucilage					305:312	mucilage	305:312	mucilage from Asplenium australasicum	305:341	Thus, mucilage from Asplenium australasicum was extracted with xylanase, glucanase and simultaneous application of both enzymatic preparations then investigated for their physicochemical properties.					
30465842	4	46	theme	6.04-6.86	612:620	arg1	%					607:607	%	607:607	%	607:607	It was observed that compared to the control, the yield of mucilage increased significantly by EAE (from 3.64% to 6.04-6.86%), while the average molecular weight also increased slightly.					
30465842	0	47	theme	mucilage	74:81	arg1	properties					60:69	the physicochemical properties	40:69	the physicochemical properties of mucilage from the fronds of Asplenium australasicum	40:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	0	48	from	fronds	92:97	arg1	mucilage					74:81	mucilage	74:81	mucilage from the fronds of Asplenium australasicum	74:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	0	48	from	fronds	92:97	arg1	properties					60:69	the physicochemical properties	40:69	the physicochemical properties of mucilage from the fronds of Asplenium australasicum	40:124	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	7	49	theme	FT-IR	1024:1028	arg1	fingerprint					1030:1040	the FT-IR fingerprint	1020:1040	the FT-IR fingerprint	1020:1040	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	0	50	dep	Effect	0:5	arg1	J.					127:128	J.	127:128	J.	127:128	Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.)					
30465842	5	51	theme	polysaccharide	801:814	arg1	efficiency					787:796	the higher extraction efficiency	765:796	the higher extraction efficiency of polysaccharide	765:814	SEM results showed that enzymes can erode the raw material which contributed to the higher extraction efficiency of polysaccharide.					
30465842	7	52	theme	mucilage	1260:1267	arg1	viscosity					1220:1228	intrinsic viscosity	1210:1228	intrinsic viscosity	1210:1228	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	7	52	theme	mucilage	1260:1267	arg1	capacity					1248:1255	water holding capacity	1234:1255	water holding capacity	1234:1255	Although the FT-IR fingerprint did not change pronouncedly due to the action of the enzymes, but the degree of polysaccharide branching seemed to be more complicated, thus significantly reducing the intrinsic viscosity and water holding capacity of mucilage.					
30465842	5	53	theme	raw	731:733	arg1	material					735:742	the raw material	727:742	the raw material which contributed to the higher extraction efficiency of polysaccharide	727:814	SEM results showed that enzymes can erode the raw material which contributed to the higher extraction efficiency of polysaccharide.					
30236720	6	0	theme	acid	957:960	arg1	contents					962:969	higher sugar and uronic acid contents	933:969	higher sugar and uronic acid contents	933:969	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	4	1	theme	gourd	616:620	arg1	BPS-F					646:650	BPS-F	646:650	BPS-F	646:650	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	1	theme	gourd	616:620	arg1	BPS-I					657:661	BPS-I	657:661	BPS-I	657:661	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	1	theme	gourd	616:620	arg1	BPS-H					639:643	BPS-H	639:643	BPS-H	639:643	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	1	theme	gourd	616:620	arg1	polysaccharides					622:636	Three water-soluble bitter gourd polysaccharides	589:636	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I)	589:662	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	0	2	theme	bioactive	62:70	arg1	polysaccharides					72:86	bioactive polysaccharides	62:86	bioactive polysaccharides	62:86	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	6	3	theme	ID-dried	907:914	arg1	gourd					923:927	ID-dried bitter gourd	907:927	ID-dried bitter gourd	907:927	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	0	4	dep	Momordica	105:113	arg1	L.					125:126	Momordica charantia L.	105:126	Momordica charantia L.	105:126	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	6	5	theme	sugar	940:944	arg1	contents					962:969	higher sugar and uronic acid contents	933:969	higher sugar and uronic acid contents	933:969	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	0	6	theme	bitter	91:96	arg1	Momordica					105:113	Momordica	105:113	Momordica	105:113	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	0	6	theme	bitter	91:96	arg1	gourd					98:102	bitter gourd	91:102	bitter gourd (Momordica charantia L.)	91:127	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	0	7	from	Effect	0:5	arg1	quality					50:56	product quality	42:56	product quality	42:56	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	0	7	from	Effect	0:5	arg1	polysaccharides					72:86	bioactive polysaccharides	62:86	bioactive polysaccharides	62:86	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	4	8	theme	drying	722:727	arg1	methods					729:735	the three drying methods	712:735	the three drying methods	712:735	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	9	dep	polysaccharides	622:636	arg1	BPS-F					646:650	BPS-F	646:650	BPS-F	646:650	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	9	dep	polysaccharides	622:636	arg1	BPS-I					657:661	BPS-I	657:661	BPS-I	657:661	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	9	dep	polysaccharides	622:636	arg1	BPS-H					639:643	BPS-H	639:643	BPS-H	639:643	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	9	dep	polysaccharides	622:636	arg1	polysaccharides					622:636	Three water-soluble bitter gourd polysaccharides	589:636	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I)	589:662	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	6	10	theme	higher	933:938	arg1	sugar					940:944	higher sugar	933:944	higher sugar	933:944	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	1	11	theme	dry	270:272	arg1	Momordica					288:296	Momordica	288:296	Momordica	288:296	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	11	theme	dry	270:272	arg1	gourd					281:285	dry bitter gourd	270:285	dry bitter gourd (Momordica charantia L.) slices	270:317	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	4	12	theme	bitter	609:614	arg1	gourd					616:620	bitter gourd	609:620	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I)	589:662	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	1	13	theme	bitter	274:279	arg1	Momordica					288:296	Momordica	288:296	Momordica	288:296	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	13	theme	bitter	274:279	arg1	gourd					281:285	dry bitter gourd	270:285	dry bitter gourd (Momordica charantia L.) slices	270:317	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	7	14	theme	bile	1045:1048	arg1	capacity					1063:1070	bile acid-binding capacity	1045:1070	bile acid-binding capacity	1045:1070	BPS-I exhibited stronger antioxidant activities and bile acid-binding capacity in vitro than BPS-H and BPS-F.					
30236720	8	15	theme	significant	1136:1146	arg1	activities					1169:1178	significant α-amylase inhibitory activities	1136:1178	significant α-amylase inhibitory activities	1136:1178	Moreover, BPS-F and BPS-I showed significant α-amylase inhibitory activities in vitro.					
30236720	0	16	theme	gourd	98:102	arg1	quality					50:56	product quality	42:56	product quality	42:56	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	0	16	theme	gourd	98:102	arg1	polysaccharides					72:86	bioactive polysaccharides	62:86	bioactive polysaccharides	62:86	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	1	17	theme	gourd	281:285	arg1	slices					312:317	dry bitter gourd (Momordica charantia L.) slices	270:317	dry bitter gourd (Momordica charantia L.) slices	270:317	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	7	18	theme	acid-binding	1050:1061	arg1	capacity					1063:1070	bile acid-binding capacity	1045:1070	bile acid-binding capacity	1045:1070	BPS-I exhibited stronger antioxidant activities and bile acid-binding capacity in vitro than BPS-H and BPS-F.					
30236720	2	19	contain	had	359:361	arg1	methods					351:357	the drying methods	340:357	the drying methods	340:357	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	2	19	contain	had	359:361	arg2	influences					375:384	significant influences	363:384	significant influences	363:384	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	6	20	contain	had	929:931	arg1	BPS-I					887:891	BPS-I	887:891	BPS-I obtained from ID-dried bitter gourd	887:927	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	6	20	contain	had	929:931	arg2	contents					962:969	higher sugar and uronic acid contents	933:969	higher sugar and uronic acid contents	933:969	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	5	21	theme	similar	774:780	arg1	characteristics					805:819	similar preliminary structural characteristics	774:819	similar preliminary structural characteristics	774:819	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	2	22	theme	drying	344:349	arg1	methods					351:357	the drying methods	340:357	the drying methods	340:357	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	1	23	theme	drying	158:163	arg1	drying					205:210	freeze drying	198:210	freeze drying (FD)	198:215	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	23	theme	drying	158:163	arg1	drying					241:246	infrared radiation drying	222:246	infrared radiation drying (ID)	222:251	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	23	theme	drying	158:163	arg1	methods					165:171	three drying methods	152:171	three drying methods	152:171	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	23	theme	drying	158:163	arg1	drying					190:195	hot-air drying	182:195	hot-air drying	182:195	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	0	24	theme	drying	20:25	arg1	methods					27:33	different drying methods	10:33	different drying methods	10:33	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	6	25	theme	bitter	916:921	arg1	gourd					923:927	ID-dried bitter gourd	907:927	ID-dried bitter gourd	907:927	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	2	26	theme	bitter	455:460	arg1	gourd					462:466	dried bitter gourd	449:466	dried bitter gourd	449:466	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	8	27	theme	α-amylase	1148:1156	arg1	activities					1169:1178	significant α-amylase inhibitory activities	1136:1178	significant α-amylase inhibitory activities	1136:1178	Moreover, BPS-F and BPS-I showed significant α-amylase inhibitory activities in vitro.					
30236720	0	28	theme	different	10:18	arg1	methods					27:33	different drying methods	10:33	different drying methods	10:33	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	1	29	theme	infrared	222:229	arg1	ID					249:250	ID	249:250	ID	249:250	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	29	theme	infrared	222:229	arg1	methods					165:171	three drying methods	152:171	three drying methods	152:171	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	29	theme	infrared	222:229	arg1	drying					241:246	infrared radiation drying	222:246	infrared radiation drying (ID)	222:251	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	2	30	theme	dried	449:453	arg1	gourd					462:466	dried bitter gourd	449:466	dried bitter gourd	449:466	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	0	31	dep	quality	50:56	arg1	the					38:40	the	38:40	the	38:40	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	5	32	theme	structural	794:803	arg1	characteristics					805:819	similar preliminary structural characteristics	774:819	similar preliminary structural characteristics	774:819	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	1	33	theme	radiation	231:239	arg1	ID					249:250	ID	249:250	ID	249:250	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	33	theme	radiation	231:239	arg1	methods					165:171	three drying methods	152:171	three drying methods	152:171	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	33	theme	radiation	231:239	arg1	drying					241:246	infrared radiation drying	222:246	infrared radiation drying (ID)	222:251	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	7	34	theme	antioxidant	1018:1028	arg1	activities					1030:1039	stronger antioxidant activities	1009:1039	stronger antioxidant activities	1009:1039	BPS-I exhibited stronger antioxidant activities and bile acid-binding capacity in vitro than BPS-H and BPS-F.					
30236720	3	35	theme	bitter	500:505	arg1	gourd					507:511	dried bitter gourd	494:511	high-quality dried bitter gourd products	481:520	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	5	36	theme	preliminary	782:792	arg1	characteristics					805:819	similar preliminary structural characteristics	774:819	similar preliminary structural characteristics	774:819	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	0	37	theme	methods	27:33	arg1	Effect					0:5	Effect	0:5	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.)	0:127	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	8	38	theme	inhibitory	1158:1167	arg1	activities					1169:1178	significant α-amylase inhibitory activities	1136:1178	significant α-amylase inhibitory activities	1136:1178	Moreover, BPS-F and BPS-I showed significant α-amylase inhibitory activities in vitro.					
30236720	3	39	theme	uniform	533:539	arg1	network					551:557	the uniform honeycomb network	529:557	the uniform honeycomb network	529:557	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	3	40	theme	gourd	507:511	arg1	products					513:520	high-quality dried bitter gourd products	481:520	high-quality dried bitter gourd products	481:520	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	2	41	theme	rehydration	408:418	arg1	ratio					420:424	rehydration ratio	408:424	rehydration ratio	408:424	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	0	42	theme	product	42:48	arg1	quality					50:56	product quality	42:56	product quality	42:56	Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices.					
30236720	3	43	theme	honeycomb	541:549	arg1	network					551:557	the uniform honeycomb network	529:557	the uniform honeycomb network	529:557	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	3	44	theme	high-quality	481:492	arg1	products					513:520	high-quality dried bitter gourd products	481:520	high-quality dried bitter gourd products	481:520	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	5	45	theme	monosaccharide	836:849	arg1	compositions					851:862	different monosaccharide compositions	826:862	different monosaccharide compositions	826:862	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	7	46	theme	stronger	1009:1016	arg1	activities					1030:1039	stronger antioxidant activities	1009:1039	stronger antioxidant activities	1009:1039	BPS-I exhibited stronger antioxidant activities and bile acid-binding capacity in vitro than BPS-H and BPS-F.					
30236720	3	47	theme	dried	494:498	arg1	gourd					507:511	dried bitter gourd	494:511	high-quality dried bitter gourd products	481:520	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	4	48	theme	bitter	687:692	arg1	gourd					694:698	the bitter gourd	683:698	the bitter gourd dried using the three drying methods	683:735	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	5	49	theme	different	826:834	arg1	compositions					851:862	different monosaccharide compositions	826:862	different monosaccharide compositions	826:862	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	1	50	theme	hot-air	182:188	arg1	drying					190:195	hot-air drying	182:195	hot-air drying	182:195	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	50	theme	hot-air	182:188	arg1	methods					165:171	three drying methods	152:171	three drying methods	152:171	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	2	51	theme	gourd	462:466	arg1	color					401:405	color	401:405	color	401:405	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	2	51	theme	gourd	462:466	arg1	ratio					420:424	rehydration ratio	408:424	rehydration ratio	408:424	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	2	51	theme	gourd	462:466	arg1	appearance					389:398	appearance	389:398	appearance	389:398	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	2	51	theme	gourd	462:466	arg1	microstructure					431:444	microstructure	431:444	microstructure of dried bitter gourd	431:466	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	5	52	theme	molecular	868:876	arg1	weights					878:884	molecular weights	868:884	molecular weights	868:884	The three polysaccharides exhibited similar preliminary structural characteristics with different monosaccharide compositions and molecular weights.					
30236720	3	53	theme	collapsed	568:576	arg1	structure					578:586	less collapsed structure	563:586	less collapsed structure	563:586	FD provided high-quality dried bitter gourd products due to the uniform honeycomb network and less collapsed structure.					
30236720	4	54	theme	water-soluble	595:607	arg1	BPS-F					646:650	BPS-F	646:650	BPS-F	646:650	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	54	theme	water-soluble	595:607	arg1	BPS-I					657:661	BPS-I	657:661	BPS-I	657:661	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	54	theme	water-soluble	595:607	arg1	BPS-H					639:643	BPS-H	639:643	BPS-H	639:643	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	4	54	theme	water-soluble	595:607	arg1	polysaccharides					622:636	Three water-soluble bitter gourd polysaccharides	589:636	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I)	589:662	Three water-soluble bitter gourd polysaccharides (BPS-H, BPS-F, and BPS-I) were obtained from the bitter gourd dried using the three drying methods.					
30236720	1	55	dep	Momordica	288:296	arg1	L.					308:309	Momordica charantia L.	288:309	Momordica charantia L.	288:309	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	2	56	theme	significant	363:373	arg1	influences					375:384	significant influences	363:384	significant influences	363:384	Results showed that the drying methods had significant influences on appearance, color, rehydration ratio, and microstructure of dried bitter gourd.					
30236720	6	57	theme	uronic	950:955	arg1	contents					962:969	higher sugar and uronic acid contents	933:969	higher sugar and uronic acid contents	933:969	BPS-I obtained from ID-dried bitter gourd had higher sugar and uronic acid contents than BPS-H and BPS-F.					
30236720	1	58	theme	freeze	198:203	arg1	FD					213:214	FD	213:214	FD	213:214	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	58	theme	freeze	198:203	arg1	methods					165:171	three drying methods	152:171	three drying methods	152:171	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
30236720	1	58	theme	freeze	198:203	arg1	drying					205:210	freeze drying	198:210	freeze drying (FD)	198:215	In this study, three drying methods, namely, hot-air drying, freeze drying (FD), and infrared radiation drying (ID), were applied to dry bitter gourd (Momordica charantia L.) slices.					
31569637	0	0	theme	Cellular	110:117	arg1	Products					119:126	Cellular Products	110:126	Cellular Products	110:126	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	6	1	theme	electrical	1320:1329	arg1	conductivity					1331:1342	enhanced electrical conductivity	1311:1342	enhanced electrical conductivity	1311:1342	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	5	2	from	moduli	1108:1113	arg1	viscosity					1079:1087	complex viscosity	1071:1087	complex viscosity	1071:1087	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	3	3	theme	surfactants-sodium	732:749	arg1	sulfate					759:765	three different surfactants-sodium dodecyl sulfate	716:765	three different surfactants-sodium dodecyl sulfate (SDS-anionic)	716:779	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	3	theme	surfactants-sodium	732:749	arg1	SDS-anionic					768:778	SDS-anionic	768:778	SDS-anionic	768:778	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	2	4	theme	carbon	384:389	arg1	nanotubes					391:399	single-walled carbon nanotubes	370:399	single-walled carbon nanotubes	370:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	5	5	theme	SWCNT	1122:1126	arg1	gels					1138:1141	the SWCNT composite gels	1118:1141	the SWCNT composite gels	1118:1141	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	4	6	theme	Raman	1009:1013	arg1	spectroscopy					1015:1026	Raman spectroscopy	1009:1026	Raman spectroscopy	1009:1026	After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy.					
31569637	3	7	theme	dodecyl	751:757	arg1	sulfate					759:765	three different surfactants-sodium dodecyl sulfate	716:765	three different surfactants-sodium dodecyl sulfate (SDS-anionic)	716:779	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	7	theme	dodecyl	751:757	arg1	SDS-anionic					768:778	SDS-anionic	768:778	SDS-anionic	768:778	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	6	8	theme	alginate	1360:1367	arg1	gels					1369:1372	alginate gels	1360:1372	alginate gels	1360:1372	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	5	9	theme	Rheometric	1029:1038	arg1	analysis					1040:1047	Rheometric analysis	1029:1047	Rheometric analysis	1029:1047	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	8	10	from	matrix	1595:1600	arg1	agglomeration					1551:1563	agglomeration	1551:1563	agglomeration	1551:1563	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	6	11	theme	structure	1262:1270	arg1	presence					1224:1231	the presence	1220:1231	the presence of a well-distributed porous structure	1220:1270	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	6	12	theme	well-distributed	1238:1253	arg1	structure					1262:1270	a well-distributed porous structure	1236:1270	a well-distributed porous structure	1236:1270	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	3	13	theme	cetyltrimethylammonium	782:803	arg1	CTAB-cationic					814:826	CTAB-cationic	814:826	CTAB-cationic	814:826	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	13	theme	cetyltrimethylammonium	782:803	arg1	bromide					805:811	cetyltrimethylammonium bromide	782:811	cetyltrimethylammonium bromide (CTAB-cationic)	782:827	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	2	14	theme	importance	297:306	arg1	parameters					283:292	the parameters	279:292	the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes	279:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	0	15	theme	Nanotube-Based	63:76	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	5	16	theme	pure	1162:1165	arg1	gels					1176:1179	pure alginate gels	1162:1179	pure alginate gels	1162:1179	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	5	17	from	increase	1059:1066	arg1	viscosity					1079:1087	complex viscosity	1071:1087	complex viscosity	1071:1087	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	2	18	theme	dose-dependent	481:494	arg1	correlation					496:506	their dose-dependent correlation	475:506	their dose-dependent correlation	475:506	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	5	19	theme	alginate	1167:1174	arg1	gels					1176:1179	pure alginate gels	1162:1179	pure alginate gels	1162:1179	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	2	20	theme	alginate-based	331:344	arg1	composite					355:363	an alginate-based hydrogel composite	328:363	an alginate-based hydrogel composite with single-walled carbon nanotubes	328:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	8	21	theme	scaffold	1623:1630	arg1	formation					1632:1640	a stable scaffold formation	1614:1640	a stable scaffold formation	1614:1640	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	2	22	theme	varying	412:418	arg1	degrees					420:426	their varying degrees	406:426	their varying degrees	406:426	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	8	23	theme	stable	1616:1621	arg1	formation					1632:1640	a stable scaffold formation	1614:1640	a stable scaffold formation	1614:1640	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	4	24	theme	SWCNT-surfactant	894:909	arg1	mixing					880:885	mixing	880:885	mixing of the SWCNT-surfactant with alginate	880:923	After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy.					
31569637	8	25	theme	alginate	1586:1593	arg1	matrix					1595:1600	the alginate matrix	1582:1600	the alginate matrix	1582:1600	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	8	26	from	agglomeration	1551:1563	arg1	matrix					1595:1600	the alginate matrix	1582:1600	the alginate matrix	1582:1600	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	0	27	theme	HiPco	36:40	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	8	28	theme	SWCNTs	1572:1577	arg1	agglomeration					1551:1563	agglomeration	1551:1563	agglomeration	1551:1563	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	2	29	theme	particulate	445:455	arg1	agglomeration					457:469	their particulate agglomeration	439:469	their particulate agglomeration	439:469	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	30	theme	agglomeration	457:469	arg1	degrees					420:426	their varying degrees	406:426	their varying degrees	406:426	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	4	31	theme	calcium	972:978	arg1	ions					980:983	divalent calcium ions	963:983	divalent calcium ions	963:983	After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy.					
31569637	2	32	with	composite	355:363	arg1	nanotubes					391:399	single-walled carbon nanotubes	370:399	single-walled carbon nanotubes	370:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	33	from	parameters	283:292	arg1	formation					315:323	the formation	311:323	the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes	311:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	0	34	theme	Carbon	56:61	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	2	35	theme	resultant	562:570	arg1	efficiency					584:593	the resultant composite's efficiency	558:593	the resultant composite's efficiency	558:593	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	5	36	theme	composite	1128:1136	arg1	gels					1138:1141	the SWCNT composite gels	1118:1141	the SWCNT composite gels	1118:1141	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	6	37	theme	enhanced	1311:1318	arg1	conductivity					1331:1342	enhanced electrical conductivity	1311:1342	enhanced electrical conductivity	1311:1342	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	5	38	from	loss	1090:1093	arg1	viscosity					1079:1087	complex viscosity	1071:1087	complex viscosity	1071:1087	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	2	39	theme	cell	511:514	arg1	viability					516:524	cell viability	511:524	cell viability	511:524	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	3	40	theme	Pluronic	834:841	arg1	nonionic					849:856	nonionic	849:856	nonionic	849:856	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	40	theme	Pluronic	834:841	arg1	F108					843:846	Pluronic F108	834:846	Pluronic F108 (nonionic)	834:857	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	0	41	theme	Single-Walled	42:54	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	5	42	theme	gels	1138:1141	arg1	loss					1090:1093	loss	1090:1093	loss	1090:1093	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	5	42	theme	gels	1138:1141	arg1	increase					1059:1066	an increase	1056:1066	an increase in complex viscosity	1056:1087	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	5	42	theme	gels	1138:1141	arg1	moduli					1108:1113	storage moduli	1100:1113	storage moduli of the SWCNT composite gels	1100:1141	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	8	43	theme	Pluronic	1504:1511	arg1	F108					1513:1516	Pluronic F108	1504:1516	Pluronic F108	1504:1516	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	8	44	from	SWCNTs	1572:1577	arg1	matrix					1595:1600	the alginate matrix	1582:1600	the alginate matrix	1582:1600	Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.					
31569637	2	45	theme	purity	431:436	arg1	degrees					420:426	their varying degrees	406:426	their varying degrees	406:426	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	0	46	theme	Alginate	78:85	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	3	47	dep	-were	858:862	arg1	utilized					864:871	utilized	864:871	-were utilized	858:871	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	6	48	theme	electron	1191:1198	arg1	microscopy					1200:1209	Scanning electron microscopy	1182:1209	Scanning electron microscopy	1182:1209	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	2	49	theme	single-walled	370:382	arg1	nanotubes					391:399	single-walled carbon nanotubes	370:399	single-walled carbon nanotubes	370:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	50	theme	correlation	496:506	arg1	degrees					420:426	their varying degrees	406:426	their varying degrees	406:426	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	5	51	theme	storage	1100:1106	arg1	moduli					1108:1113	storage moduli	1100:1113	storage moduli of the SWCNT composite gels	1100:1141	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	6	52	theme	SWCNT-gel	1281:1289	arg1	composites					1291:1300	all SWCNT-gel composites	1277:1300	all SWCNT-gel composites	1277:1300	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	6	53	theme	Scanning	1182:1189	arg1	microscopy					1200:1209	Scanning electron microscopy	1182:1209	Scanning electron microscopy	1182:1209	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	3	54	theme	homogenous	652:661	arg1	dispersion					663:672	their homogenous dispersion	646:672	their homogenous dispersion	646:672	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	4	55	theme	divalent	963:970	arg1	ions					980:983	divalent calcium ions	963:983	divalent calcium ions	963:983	After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy.					
31569637	3	56	theme	different	722:730	arg1	sulfate					759:765	three different surfactants-sodium dodecyl sulfate	716:765	three different surfactants-sodium dodecyl sulfate (SDS-anionic)	716:779	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	56	theme	different	722:730	arg1	SDS-anionic					768:778	SDS-anionic	768:778	SDS-anionic	768:778	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	2	57	theme	composite	355:363	arg1	formation					315:323	the formation	311:323	the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes	311:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	4	58	with	mixing	880:885	arg1	alginate					916:923	alginate	916:923	alginate	916:923	After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy.					
31569637	3	59	theme	SWCNT	709:713	arg1	agglomeration					688:700	agglomeration	688:700	agglomeration of the SWCNT	688:713	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	F108					843:846	Pluronic F108	834:846	Pluronic F108 (nonionic)	834:857	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	sulfate					759:765	three different surfactants-sodium dodecyl sulfate	716:765	three different surfactants-sodium dodecyl sulfate (SDS-anionic)	716:779	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	bromide					805:811	cetyltrimethylammonium bromide	782:811	cetyltrimethylammonium bromide (CTAB-cationic)	782:827	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	CTAB-cationic					814:826	CTAB-cationic	814:826	CTAB-cationic	814:826	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	nonionic					849:856	nonionic	849:856	nonionic	849:856	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	3	59	theme	SWCNT	709:713	arg1	SDS-anionic					768:778	SDS-anionic	768:778	SDS-anionic	768:778	To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized.					
31569637	6	60	theme	porous	1255:1260	arg1	structure					1262:1270	a well-distributed porous structure	1236:1270	a well-distributed porous structure	1236:1270	Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels.					
31569637	2	61	contain	have	540:543	arg1	degrees					420:426	their varying degrees	406:426	their varying degrees	406:426	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	61	contain	have	540:543	arg2	impact					548:553	an impact	545:553	an impact on the resultant composite's efficiency and effectiveness towards cell-therapy	545:632	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	61	contain	have	540:543	arg1	all					527:529	all	527:529	all	527:529	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	2	62	theme	hydrogel	346:353	arg1	composite					355:363	an alginate-based hydrogel composite	328:363	an alginate-based hydrogel composite with single-walled carbon nanotubes	328:399	Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy.					
31569637	5	63	with	comparison	1146:1155	arg1	gels					1176:1179	pure alginate gels	1162:1179	pure alginate gels	1162:1179	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	1	64	theme	-alginate	242:250	arg1	composites					261:270	ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites	188:270	ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites	188:270	In this study, we designed, synthesized, and characterized ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites.					
31569637	0	65	theme	Composites	96:105	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.	0:127	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	5	66	theme	complex	1071:1077	arg1	viscosity					1079:1087	complex viscosity	1071:1087	complex viscosity	1071:1087	Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels.					
31569637	1	67	theme	hydrogel	252:259	arg1	composites					261:270	ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites	188:270	ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites	188:270	In this study, we designed, synthesized, and characterized ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites.					
31569637	0	68	theme	Hydrogel	87:94	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31569637	0	69	theme	Surfactant-Dispersed	15:34	arg1	Composites					96:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites	15:105	Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products.					
31429260	7	0	theme	sample	1888:1893	arg1	method					1907:1912	our sample preparation method	1884:1912	our sample preparation method along with the achieved separation and in-depth structural characterization	1884:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	3	1	theme	MS	765:766	arg1	method					768:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method	672:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides	672:862	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	4	2	theme	dihydroxybenzoic	974:989	arg1	nanoparticles					1020:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	5	3	theme	diagnostic	1272:1281	arg1	ions					1318:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	7	4	theme	demonstrated	1857:1868	arg1	simplicity					1870:1879	the demonstrated simplicity	1853:1879	the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization	1853:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	5	theme	observed	1110:1117	arg1	CV					1146:1147	<20% CV	1141:1147	<20% CV	1141:1147	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	5	theme	observed	1110:1117	arg1	reproducibility					1124:1138	the observed high reproducibility	1106:1138	the observed high reproducibility (<20% CV)	1106:1148	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	6	from	abundance	1703:1711	arg1	samples					1716:1722	samples	1716:1722	samples from individual donors at different lactation times	1716:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	6	from	abundance	1703:1711	arg1	donors					1740:1745	individual donors	1729:1745	individual donors at different lactation times	1729:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	1	7	theme	structure-function	254:271	arg1	relationships					273:285	their structure-function relationships	248:285	their structure-function relationships	248:285	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	4	8	theme	@	1038:1038	arg1	MNPs					1039:1042	DHB@MNPs	1035:1042	DHB@MNPs	1035:1042	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	8	theme	@	1038:1038	arg1	dispersion					921:930	good dispersion	916:930	good dispersion	916:930	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	9	theme	signal	1170:1175	arg1	enhancement					1177:1187	28-fold signal enhancement	1162:1187	28-fold signal enhancement	1162:1187	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	10	from	variability	1668:1678	arg1	abundance					1703:1711	the oligosaccharide abundance	1683:1711	the oligosaccharide abundance	1683:1711	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	7	11	theme	samples	2070:2076	arg1	screening					2030:2038	the rapid screening	2020:2038	the rapid screening of other oligosaccharide-rich samples	2020:2076	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	12	from	dispersion	921:930	arg1	plate					1056:1060	the TLC plate	1048:1060	the TLC plate	1048:1060	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	7	13	theme	other	2043:2047	arg1	samples					2070:2076	other oligosaccharide-rich samples	2043:2076	other oligosaccharide-rich samples	2043:2076	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	14	theme	magnetic	1011:1018	arg1	nanoparticles					1020:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	15	theme	lactation	1760:1768	arg1	times					1770:1774	different lactation times	1750:1774	different lactation times	1750:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	16	theme	ionic	675:679	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	16	theme	ionic	675:679	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	1	17	from	relationships	273:285	arg1	health					308:313	health	308:313	health	308:313	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	1	17	from	relationships	273:285	arg1	diseases					327:334	various diseases	319:334	various diseases	319:334	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	1	18	theme	in-depth	173:180	arg1	characterization					182:197	The in-depth characterization	169:197	The in-depth characterization of glycan structures	169:218	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	1	18	theme	in-depth	173:180	arg1	crucial					223:229	crucial	223:229	crucial	223:229	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	4	19	theme	spot	1067:1070	arg1	homogeneity					1072:1082	spot homogeneity	1067:1082	spot homogeneity	1067:1082	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	20	theme	individual	1729:1738	arg1	donors					1740:1745	individual donors	1729:1745	individual donors at different lactation times	1729:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	5	21	theme	composition	1367:1377	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	3	22	theme	efficient	875:883	arg1	profiling					885:893	efficient profiling	875:893	efficient profiling	875:893	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	0	23	theme	Ionic	78:82	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	1	24	theme	structures	209:218	arg1	characterization					182:197	The in-depth characterization	169:197	The in-depth characterization of glycan structures	169:218	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	1	24	theme	structures	209:218	arg1	crucial					223:229	crucial	223:229	crucial	223:229	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	0	25	theme	Nanomatrix-Assisted	102:120	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	2	26	theme	structural	621:630	arg1	similarity					632:641	their high structural similarity	610:641	their high structural similarity	610:641	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	5	27	theme	on-spot	1333:1339	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	0	28	theme	Desorption-Ionization	128:148	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	7	29	theme	rapid	2024:2028	arg1	screening					2030:2038	the rapid screening	2020:2038	the rapid screening of other oligosaccharide-rich samples	2020:2076	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	2	30	theme	complex	476:482	arg1	mixtures					484:491	complex mixtures	476:491	complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity	476:641	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	7	31	used	used	2011:2014	arg2	approach					1995:2002	our approach	1991:2002	our approach	1991:2002	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	32	from	stabilization	936:948	arg1	plate					1056:1060	the TLC plate	1048:1060	the TLC plate	1048:1060	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	33	theme	oligosaccharide	1687:1701	arg1	abundance					1703:1711	the oligosaccharide abundance	1683:1711	the oligosaccharide abundance	1683:1711	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	4	34	theme	high	1119:1122	arg1	CV					1146:1147	<20% CV	1141:1147	<20% CV	1141:1147	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	34	theme	high	1119:1122	arg1	reproducibility					1124:1138	the observed high reproducibility	1106:1138	the observed high reproducibility (<20% CV)	1106:1148	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	0	35	theme	Liquid-Stabilized	84:100	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	0	36	theme	Direct	0:5	arg1	Profiling					23:31	Direct Oligosaccharide Profiling	0:31	Direct Oligosaccharide Profiling	0:31	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	5	37	theme	sequence	1380:1387	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	2	38	theme	due	510:512	arg1	carbohydrates					496:508	carbohydrates	496:508	carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity	496:641	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	39	from	advances	345:352	arg1	analysis					363:370	rapid analysis	357:370	rapid analysis	357:370	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	40	from	milk	1628:1631	arg1	identification					1579:1592	the identification	1575:1592	the identification of 25 oligosaccharides from human milk	1575:1631	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	40	from	milk	1628:1631	arg1	oligosaccharides					1600:1615	25 oligosaccharides	1597:1615	25 oligosaccharides from human milk	1597:1631	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	41	from	donors	1740:1745	arg1	samples					1716:1722	samples	1716:1722	samples from individual donors at different lactation times	1716:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	41	from	donors	1740:1745	arg1	abundance					1703:1711	the oligosaccharide abundance	1683:1711	the oligosaccharide abundance	1683:1711	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	42	theme	chromatography	738:751	arg1	method					768:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method	672:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides	672:862	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	5	43	theme	branching	1390:1398	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	0	44	theme	Thin-Layer	39:48	arg1	Chromatography					50:63	Thin-Layer Chromatography	39:63	Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	39:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	6	45	theme	glycan	1483:1488	arg1	samples					1490:1496	glycan samples	1483:1496	glycan samples	1483:1496	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	2	46	theme	spectrometry	437:448	arg1	utility					377:383	the utility	373:383	the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	373:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	5	47	theme	separate	1223:1230	arg1	glycans					1241:1247	separate isomeric glycans	1223:1247	separate isomeric glycans	1223:1247	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	7	48	theme	structural	1962:1971	arg1	characterization					1973:1988	in-depth structural characterization	1953:1988	in-depth structural characterization	1953:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	3	49	theme	-MALDI	758:763	arg1	method					768:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method	672:773	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides	672:862	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	2	50	theme	desorption/ionization	410:430	arg1	spectrometry					437:448	matrix-assisted laser desorption/ionization mass spectrometry	388:448	matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	388:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	50	theme	desorption/ionization	410:430	arg1	MS					457:458	MALDI MS	451:458	MALDI MS	451:458	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	51	theme	integrated	1546:1555	arg1	TLC					1523:1525	TLC	1523:1525	TLC	1523:1525	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	51	theme	integrated	1546:1555	arg1	platform					1557:1564	our integrated platform	1542:1564	our integrated platform	1542:1564	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	5	52	theme	glycosidic	1283:1292	arg1	ions					1318:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	7	53	theme	preparation	1895:1905	arg1	method					1907:1912	our sample preparation method	1884:1912	our sample preparation method along with the achieved separation and in-depth structural characterization	1884:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	54	theme	28-fold	1162:1168	arg1	enhancement					1177:1187	28-fold signal enhancement	1162:1187	28-fold signal enhancement	1162:1187	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	5	55	theme	cross-ring	1298:1307	arg1	ions					1318:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	4	56	theme	%	1144:1144	arg1	CV					1146:1147	<20% CV	1141:1147	<20% CV	1141:1147	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	56	theme	%	1144:1144	arg1	reproducibility					1124:1138	the observed high reproducibility	1106:1138	the observed high reproducibility (<20% CV)	1106:1148	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	3	57	theme	simultaneous	779:790	arg1	separation					802:811	simultaneous and rapid separation	779:811	simultaneous and rapid separation	779:811	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	5	58	theme	separated	1432:1440	arg1	spot					1442:1445	each separated spot	1427:1445	each separated spot	1427:1445	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	2	59	theme	MALDI	451:455	arg1	spectrometry					437:448	matrix-assisted laser desorption/ionization mass spectrometry	388:448	matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	388:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	59	theme	MALDI	451:455	arg1	MS					457:458	MALDI MS	451:458	MALDI MS	451:458	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	60	theme	tandem	1531:1536	arg1	MS					1538:1539	tandem MS	1531:1539	tandem MS	1531:1539	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	5	61	theme	@	1257:1257	arg1	MNPs					1258:1261	the DHB@MNPs	1250:1261	the DHB@MNPs	1250:1261	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	4	62	theme	spin	958:961	arg1	coating					963:969	the spin coating	954:969	the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles	954:1032	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	7	63	dep	along	1914:1918	arg1	with					1920:1923	with	1920:1923	with	1920:1923	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	2	64	theme	rapid	357:361	arg1	analysis					363:370	rapid analysis	357:370	rapid analysis	357:370	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	65	theme	oligosaccharides	1600:1615	arg1	identification					1579:1592	the identification	1575:1592	the identification of 25 oligosaccharides from human milk	1575:1631	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	66	theme	rapid	796:800	arg1	separation					802:811	simultaneous and rapid separation	779:811	simultaneous and rapid separation	779:811	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	2	67	theme	matrix-assisted	388:402	arg1	spectrometry					437:448	matrix-assisted laser desorption/ionization mass spectrometry	388:448	matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	388:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	67	theme	matrix-assisted	388:402	arg1	MS					457:458	MALDI MS	451:458	MALDI MS	451:458	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	4	68	theme	DHB	1035:1037	arg1	MNPs					1039:1042	DHB@MNPs	1035:1042	DHB@MNPs	1035:1042	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	68	theme	DHB	1035:1037	arg1	dispersion					921:930	good dispersion	916:930	good dispersion	916:930	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	69	from	times	1770:1774	arg1	donors					1740:1745	individual donors	1729:1745	individual donors at different lactation times	1729:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	70	theme	liquid	681:686	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	70	theme	liquid	681:686	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	1	71	from	effects	297:303	arg1	health					308:313	health	308:313	health	308:313	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	1	71	from	effects	297:303	arg1	diseases					327:334	various diseases	319:334	various diseases	319:334	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	7	72	theme	oligosaccharide-rich	2049:2068	arg1	samples					2070:2076	other oligosaccharide-rich samples	2043:2076	other oligosaccharide-rich samples	2043:2076	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	4	73	theme	acid-functionalized	991:1009	arg1	nanoparticles					1020:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	dihydroxybenzoic acid-functionalized magnetic nanoparticles	974:1032	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	74	theme	different	1750:1758	arg1	times					1770:1774	different lactation times	1750:1774	different lactation times	1750:1774	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	5	75	theme	structural	1341:1350	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	3	76	theme	oligosaccharides	847:862	arg1	detection					814:822	detection	814:822	detection	814:822	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	76	theme	oligosaccharides	847:862	arg1	identification					829:842	identification	829:842	identification	829:842	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	76	theme	oligosaccharides	847:862	arg1	separation					802:811	simultaneous and rapid separation	779:811	simultaneous and rapid separation	779:811	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	77	theme	-stabilized	692:702	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	77	theme	-stabilized	692:702	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	6	78	theme	heatmap	1638:1644	arg1	analysis					1646:1653	heatmap analysis	1638:1653	heatmap analysis	1638:1653	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	4	79	theme	nanoparticles	1020:1032	arg1	coating					963:969	the spin coating	954:969	the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles	954:1032	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	6	80	theme	chemical	1456:1463	arg1	derivatization					1465:1478	chemical derivatization	1456:1478	chemical derivatization of glycan samples	1456:1496	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	81	theme	IL	689:690	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	81	theme	IL	689:690	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	1	82	theme	glycan	202:207	arg1	structures					209:218	glycan structures	202:218	glycan structures	202:218	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	0	83	theme	Laser	122:126	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	6	84	theme	human	1622:1626	arg1	milk					1628:1631	human milk	1622:1631	human milk	1622:1631	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	85	dep	microbiota	1812:1821	arg1	the					1808:1810	the	1808:1810	the	1808:1810	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	0	86	theme	Mass	150:153	arg1	Spectrometry					155:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry	78:166	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	2	87	theme	high	616:619	arg1	similarity					632:641	their high structural similarity	610:641	their high structural similarity	610:641	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	4	88	theme	TLC	1052:1054	arg1	plate					1056:1060	the TLC plate	1048:1060	the TLC plate	1048:1060	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	5	89	theme	linkage	1405:1411	arg1	elucidation					1352:1362	on-spot structural elucidation	1333:1362	on-spot structural elucidation of composition, sequence, branching, and linkage of glycans	1333:1422	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	0	90	theme	Oligosaccharide	7:21	arg1	Profiling					23:31	Direct Oligosaccharide Profiling	0:31	Direct Oligosaccharide Profiling	0:31	Direct Oligosaccharide Profiling Using Thin-Layer Chromatography Coupled with Ionic Liquid-Stabilized Nanomatrix-Assisted Laser Desorption-Ionization Mass Spectrometry.					
31429260	2	91	theme	low	523:525	arg1	efficiency					538:547	their low ionization efficiency	517:547	their low ionization efficiency	517:547	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	92	theme	glycan	1499:1504	arg1	visualization					1506:1518	glycan visualization	1499:1518	glycan visualization by TLC and tandem MS, our integrated platform,	1499:1565	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	93	from	identification	1579:1592	arg1	milk					1628:1631	human milk	1622:1631	human milk	1622:1631	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	3	94	theme	thin-layer	727:736	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	94	theme	thin-layer	727:736	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	2	95	theme	carbohydrates	496:508	arg1	mixtures					484:491	complex mixtures	476:491	complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity	476:641	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	6	96	theme	samples	1490:1496	arg1	derivatization					1465:1478	chemical derivatization	1456:1478	chemical derivatization of glycan samples	1456:1496	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	5	97	theme	DHB	1254:1256	arg1	MNPs					1258:1261	the DHB@MNPs	1250:1261	the DHB@MNPs	1250:1261	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	3	98	theme	nanomatrix-decorated	705:724	arg1	TLC					754:756	TLC	754:756	TLC	754:756	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	3	98	theme	nanomatrix-decorated	705:724	arg1	chromatography					738:751	an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography	672:751	chromatography	738:751	In this study, we developed an ionic liquid (IL)-stabilized, nanomatrix-decorated, thin-layer chromatography (TLC)-MALDI MS method for simultaneous and rapid separation, detection, and identification of oligosaccharides to achieve efficient profiling.					
31429260	7	99	theme	in-depth	1953:1960	arg1	characterization					1973:1988	in-depth structural characterization	1953:1988	in-depth structural characterization	1953:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	7	100	theme	achieved	1929:1936	arg1	separation					1938:1947	the achieved separation	1925:1947	the achieved separation	1925:1947	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	6	101	theme	infants	1839:1845	arg1	microbiota					1812:1821	microbiota	1812:1821	microbiota	1812:1821	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	6	101	theme	infants	1839:1845	arg1	immunity					1827:1834	immunity	1827:1834	immunity	1827:1834	Without chemical derivatization of glycan samples, glycan visualization by TLC and tandem MS, our integrated platform, allowed the identification of 25 oligosaccharides from human milk, and heatmap analysis revealed the variability in the oligosaccharide abundance in samples from individual donors at different lactation times, which may provide insight into the microbiota and immunity of infants.					
31429260	4	102	theme	good	916:919	arg1	MNPs					1039:1042	DHB@MNPs	1035:1042	DHB@MNPs	1035:1042	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	4	102	theme	good	916:919	arg1	dispersion					921:930	good dispersion	916:930	good dispersion	916:930	The IL demonstrated good dispersion and stabilization for the spin coating of dihydroxybenzoic acid-functionalized magnetic nanoparticles (DHB@MNPs) on the TLC plate with spot homogeneity, which contributed to the observed high reproducibility (<20% CV) and 12- and 28-fold signal enhancement.					
31429260	2	103	theme	mass	432:435	arg1	spectrometry					437:448	matrix-assisted laser desorption/ionization mass spectrometry	388:448	matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	388:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	103	theme	mass	432:435	arg1	MS					457:458	MALDI MS	451:458	MALDI MS	451:458	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	5	104	theme	isomeric	1232:1239	arg1	glycans					1241:1247	separate isomeric glycans	1223:1247	separate isomeric glycans	1223:1247	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	7	105	theme	separation	1938:1947	arg1	simplicity					1870:1879	the demonstrated simplicity	1853:1879	the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization	1853:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	5	106	theme	glycans	1416:1422	arg1	branching					1390:1398	branching	1390:1398	branching	1390:1398	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	5	106	theme	glycans	1416:1422	arg1	linkage					1405:1411	linkage	1405:1411	linkage	1405:1411	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	5	106	theme	glycans	1416:1422	arg1	sequence					1380:1387	sequence	1380:1387	sequence	1380:1387	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	5	106	theme	glycans	1416:1422	arg1	composition					1367:1377	composition	1367:1377	composition	1367:1377	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
31429260	2	107	theme	laser	404:408	arg1	spectrometry					437:448	matrix-assisted laser desorption/ionization mass spectrometry	388:448	matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)	388:459	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	2	107	theme	laser	404:408	arg1	MS					457:458	MALDI MS	451:458	MALDI MS	451:458	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	7	108	theme	method	1907:1912	arg1	simplicity					1870:1879	the demonstrated simplicity	1853:1879	the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization	1853:1988	With the demonstrated simplicity of our sample preparation method along with the achieved separation and in-depth structural characterization, our approach can be used for the rapid screening of other oligosaccharide-rich samples.					
31429260	1	109	theme	various	319:325	arg1	diseases					327:334	various diseases	319:334	various diseases	319:334	The in-depth characterization of glycan structures is crucial to understanding their structure-function relationships and their effects on health and various diseases.					
31429260	2	110	theme	ionization	527:536	arg1	efficiency					538:547	their low ionization efficiency	517:547	their low ionization efficiency	517:547	Despite advances in rapid analysis, the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is limited for complex mixtures of carbohydrates due to their low ionization efficiency and the difficulty in separating oligosaccharides because of their high structural similarity.					
31429260	5	111	theme	cleavage	1309:1316	arg1	ions					1318:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	diagnostic glycosidic and cross-ring cleavage ions	1272:1321	Although the TLC was not able to separate isomeric glycans, the DHB@MNPs generate diagnostic glycosidic and cross-ring cleavage ions, enabling on-spot structural elucidation of composition, sequence, branching, and linkage of glycans in each separated spot.					
29463789	0	0	from	optimization	21:32	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	0	from	optimization	21:32	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	1	1	from	influence	329:337	arg1	properties					393:402	physicochemical properties	377:402	physicochemical properties	377:402	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	1	1	from	influence	329:337	arg1	abilities					420:428	antioxidant abilities	408:428	antioxidant abilities	408:428	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	3	2	theme	temperature	667:677	arg1	25 mL/g					628:634	25 mL/g	628:634	25 mL/g	628:634	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	2	theme	temperature	667:677	arg1	time					648:651	extraction time	637:651	extraction time of 61 min and temperature of 75 °C. Under these parameters	637:710	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	0	3	dep	Astragalus	145:154	arg1	methodology					182:192	cicer L. Response surface methodology	156:192	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	4	dep	ratio	619:623	arg1	to					603:604	to	603:604	to	603:604	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	1	5	theme	drying	352:357	arg1	techniques					359:368	different drying techniques	342:368	different drying techniques	342:368	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	1	6	theme	techniques	359:368	arg1	influence					329:337	The influence	325:337	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs	325:436	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	7	7	theme	1 mg/mL	1163:1169	arg1	concentration					1146:1158	the determined concentration	1131:1158	the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS	1131:1215	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	8	8	theme	L.	1375:1376	arg1	polysaccharides					1378:1392	Astragalus cicer L. polysaccharides	1358:1392	Astragalus cicer L. polysaccharides	1358:1392	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	3	9	theme	raw	606:608	arg1	ratio					619:623	water to raw material ratio	597:623	water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97%	597:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	2	10	theme	freeze	509:514	arg1	FD					524:525	FD	524:525	FD	524:525	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	2	10	theme	freeze	509:514	arg1	drying					516:521	freeze drying	509:521	freeze drying (FD)	509:526	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	6	11	contain	had	1002:1004	arg2	activities					1027:1036	the best antioxidant activities	1006:1036	the best antioxidant activities	1006:1036	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	11	contain	had	1002:1004	arg1	ACPs					962:965	ACPs	962:965	ACPs dried with the FD method (FD-ACPs)	962:1000	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	11	contain	had	1002:1004	arg2	related					1054:1060	related	1054:1060	related	1054:1060	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	4	12	theme	ACPs	822:825	arg1	compositions					796:807	the monosaccharide compositions	777:807	the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques	777:860	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	4	12	theme	ACPs	822:825	arg1	identical					867:875	identical	867:875	identical	867:875	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	6	13	theme	molecular	1079:1087	arg1	weight					1089:1094	their smaller molecular weight	1065:1094	their smaller molecular weight	1065:1094	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	14	theme	higher	1100:1105	arg1	content					1119:1125	higher uronic acid content	1100:1125	higher uronic acid content	1100:1125	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	7	15	theme	radical	1222:1228	arg1	capacities					1241:1250	free radical scavenging capacities	1217:1250	free radical scavenging capacities of FD-ACPs	1217:1261	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	0	16	theme	antioxidant	98:108	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	17	theme	extraction	637:646	arg1	25 mL/g					628:634	25 mL/g	628:634	25 mL/g	628:634	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	17	theme	extraction	637:646	arg1	time					648:651	extraction time	637:651	extraction time of 61 min and temperature of 75 °C. Under these parameters	637:710	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	6	18	theme	best	1010:1013	arg1	related					1054:1060	related	1054:1060	related	1054:1060	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	18	theme	best	1010:1013	arg1	activities					1027:1036	the best antioxidant activities	1006:1036	the best antioxidant activities	1006:1036	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	7	19	theme	reducing	1183:1190	arg1	power					1192:1196	the ferric reducing power	1172:1196	the ferric reducing power	1172:1196	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	0	20	theme	polysaccharides	124:138	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	20	theme	polysaccharides	124:138	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	1	21	theme	physicochemical	377:391	arg1	properties					393:402	physicochemical properties	377:402	physicochemical properties	377:402	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	0	22	theme	extraction	256:265	arg1	parameters					267:276	the extraction parameters	252:276	the extraction parameters of Astragalus cicer L. polysaccharides (ACPs)	252:322	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	7	23	theme	scavenging	1230:1239	arg1	capacities					1241:1250	free radical scavenging capacities	1217:1250	free radical scavenging capacities of FD-ACPs	1217:1261	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	2	24	theme	hot	479:481	arg1	air					483:485	hot air	479:485	hot air (HD)	479:490	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	2	24	theme	hot	479:481	arg1	HD					488:489	HD	488:489	HD	488:489	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	0	25	theme	Astragalus	281:290	arg1	ACPs					318:321	ACPs	318:321	ACPs	318:321	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	25	theme	Astragalus	281:290	arg1	polysaccharides					301:315	Astragalus cicer L. polysaccharides	281:315	Astragalus cicer L. polysaccharides (ACPs)	281:322	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	4	26	dep	HD	838:839	arg1	techniques					851:860	techniques	851:860	techniques	851:860	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	0	27	theme	condition	11:19	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	28	from	effects	38:44	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	28	from	effects	38:44	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	7	29	theme	determined	1135:1144	arg1	concentration					1146:1158	the determined concentration	1131:1158	the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS	1131:1215	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	6	30	theme	smaller	1071:1077	arg1	weight					1089:1094	their smaller molecular weight	1065:1094	their smaller molecular weight	1065:1094	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	3	31	theme	75 °C.	682:687	arg1	temperature					667:677	temperature	667:677	temperature	667:677	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	31	theme	75 °C.	682:687	arg1	61 min					656:661	61 min	656:661	61 min	656:661	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	7	32	theme	free	1217:1220	arg1	capacities					1241:1250	free radical scavenging capacities	1217:1250	free radical scavenging capacities of FD-ACPs	1217:1261	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	0	33	theme	Extraction	0:9	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	4	34	theme	monosaccharide	781:794	arg1	compositions					796:807	the monosaccharide compositions	777:807	the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques	777:860	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	4	34	theme	monosaccharide	781:794	arg1	identical					867:875	identical	867:875	identical	867:875	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	0	35	theme	L.	298:299	arg1	ACPs					318:321	ACPs	318:321	ACPs	318:321	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	35	theme	L.	298:299	arg1	polysaccharides					301:315	Astragalus cicer L. polysaccharides	281:315	Astragalus cicer L. polysaccharides (ACPs)	281:322	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	36	theme	optimal	541:547	arg1	conditions					549:558	The optimal conditions	537:558	The optimal conditions for ACPs extraction	537:578	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	37	theme	material	610:617	arg1	ratio					619:623	water to raw material ratio	597:623	water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97%	597:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	8	38	theme	good	1328:1331	arg1	choice					1333:1338	a good choice	1326:1338	a good choice for the drying of Astragalus cicer L. polysaccharides	1326:1392	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	8	38	theme	good	1328:1331	arg1	FD					1319:1320	FD	1319:1320	FD	1319:1320	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	0	39	theme	cicer	292:296	arg1	ACPs					318:321	ACPs	318:321	ACPs	318:321	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	39	theme	cicer	292:296	arg1	polysaccharides					301:315	Astragalus cicer L. polysaccharides	281:315	Astragalus cicer L. polysaccharides (ACPs)	281:322	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	4	40	theme	HPLC	751:754	arg1	analysis					756:763	HPLC analysis	751:763	HPLC analysis	751:763	HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical.					
29463789	1	41	theme	antioxidant	408:418	arg1	abilities					420:428	antioxidant abilities	408:428	antioxidant abilities	408:428	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	6	42	theme	FD	982:983	arg1	FD-ACPs					993:999	FD-ACPs	993:999	FD-ACPs	993:999	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	42	theme	FD	982:983	arg1	method					985:990	the FD method	978:990	the FD method (FD-ACPs)	978:1000	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	0	43	theme	L.	162:163	arg1	methodology					182:192	cicer L. Response surface methodology	156:192	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	44	from	properties	83:92	arg1	RSM					195:197	RSM	195:197	RSM	195:197	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	44	from	properties	83:92	arg1	Astragalus					145:154	Astragalus	145:154	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	45	theme	ACPs	564:567	arg1	extraction					569:578	ACPs extraction	564:578	ACPs extraction	564:578	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	6	46	theme	uronic	1107:1112	arg1	content					1119:1125	higher uronic acid content	1100:1125	higher uronic acid content	1100:1125	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	1	47	dep	properties	393:402	arg1	the					373:375	the	373:375	the	373:375	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	0	48	theme	cicer	156:160	arg1	methodology					182:192	cicer L. Response surface methodology	156:192	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	49	theme	water	597:601	arg1	ratio					619:623	water to raw material ratio	597:623	water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97%	597:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	8	50	theme	cicer	1369:1373	arg1	polysaccharides					1378:1392	Astragalus cicer L. polysaccharides	1358:1392	Astragalus cicer L. polysaccharides	1358:1392	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	2	51	theme	drying	516:521	arg1	methods					528:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	7	52	theme	DPPH	1203:1206	arg1	concentration					1146:1158	the determined concentration	1131:1158	the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS	1131:1215	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	0	53	theme	methods	56:62	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	53	theme	methods	56:62	arg1	effects					38:44	effects	38:44	effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables	38:224	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	54	theme	surface	174:180	arg1	methodology					182:192	cicer L. Response surface methodology	156:192	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	1	55	theme	ACPs	433:436	arg1	properties					393:402	physicochemical properties	377:402	physicochemical properties	377:402	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	1	55	theme	ACPs	433:436	arg1	abilities					420:428	antioxidant abilities	408:428	antioxidant abilities	408:428	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	8	56	theme	Astragalus	1358:1367	arg1	polysaccharides					1378:1392	Astragalus cicer L. polysaccharides	1358:1392	Astragalus cicer L. polysaccharides	1358:1392	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	0	57	theme	polysaccharides	301:315	arg1	parameters					267:276	the extraction parameters	252:276	the extraction parameters of Astragalus cicer L. polysaccharides (ACPs)	252:322	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	7	58	theme	power	1192:1196	arg1	concentration					1146:1158	the determined concentration	1131:1158	the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS	1131:1215	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	0	59	theme	drying	49:54	arg1	methods					56:62	drying methods	49:62	drying methods	49:62	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	60	theme	Response	165:172	arg1	methodology					182:192	cicer L. Response surface methodology	156:192	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	3	61	theme	25 mL/g	628:634	arg1	ratio					619:623	water to raw material ratio	597:623	water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97%	597:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	62	theme	ACPs	716:719	arg1	25 mL/g					628:634	25 mL/g	628:634	25 mL/g	628:634	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	62	theme	ACPs	716:719	arg1	yield					721:725	an ACPs yield	713:725	an ACPs yield of 10.97%	713:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	7	63	theme	FD-ACPs	1255:1261	arg1	capacities					1241:1250	free radical scavenging capacities	1217:1250	free radical scavenging capacities of FD-ACPs	1217:1261	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	7	64	theme	ferric	1176:1181	arg1	power					1192:1196	the ferric reducing power	1172:1196	the ferric reducing power	1172:1196	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	5	65	theme	antioxidant	903:913	arg1	abilities					915:923	antioxidant abilities	903:923	antioxidant abilities	903:923	The three ACPs exhibited antioxidant abilities in a concentration-dependent manner.					
29463789	0	66	from	activities	110:119	arg1	RSM					195:197	RSM	195:197	RSM	195:197	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	66	from	activities	110:119	arg1	Astragalus					145:154	Astragalus	145:154	Astragalus cicer L. Response surface methodology (RSM)	145:198	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	2	67	theme	vacuum	493:498	arg1	methods					528:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	0	68	theme	physicochemical	67:81	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	7	69	from	concentration	1146:1158	arg1	%					1280:1280	0.762, 75.30%	1268:1280	%	1280:1280	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	7	69	from	concentration	1146:1158	arg1	%					1291:1291	99.21%	1286:1291	99.21%	1286:1291	At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively.					
29463789	6	70	theme	acid	1114:1117	arg1	content					1119:1125	higher uronic acid content	1100:1125	higher uronic acid content	1100:1125	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	0	71	from	Astragalus	145:154	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	71	from	Astragalus	145:154	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	71	from	Astragalus	145:154	arg1	variables					216:224	three variables	210:224	three variables	210:224	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	0	71	from	Astragalus	145:154	arg1	polysaccharides					124:138	polysaccharides	124:138	polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables	124:224	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs).					
29463789	2	72	theme	air	483:485	arg1	methods					528:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	hot air (HD), vacuum (VD) and freeze drying (FD) methods	479:534	The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods.					
29463789	1	73	theme	different	342:350	arg1	techniques					359:368	different drying techniques	342:368	different drying techniques	342:368	The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated.					
29463789	5	74	theme	concentration-dependent	930:952	arg1	manner					954:959	a concentration-dependent manner	928:959	a concentration-dependent manner	928:959	The three ACPs exhibited antioxidant abilities in a concentration-dependent manner.					
29463789	3	75	theme	61 min	656:661	arg1	25 mL/g					628:634	25 mL/g	628:634	25 mL/g	628:634	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	75	theme	61 min	656:661	arg1	time					648:651	extraction time	637:651	extraction time of 61 min and temperature of 75 °C. Under these parameters	637:710	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	6	76	theme	antioxidant	1015:1025	arg1	related					1054:1060	related	1054:1060	related	1054:1060	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	6	76	theme	antioxidant	1015:1025	arg1	activities					1027:1036	the best antioxidant activities	1006:1036	the best antioxidant activities	1006:1036	ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content.					
29463789	8	77	theme	polysaccharides	1378:1392	arg1	drying					1348:1353	the drying	1344:1353	the drying of Astragalus cicer L. polysaccharides	1344:1392	Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.					
29463789	3	78	theme	%	735:735	arg1	25 mL/g					628:634	25 mL/g	628:634	25 mL/g	628:634	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
29463789	3	78	theme	%	735:735	arg1	yield					721:725	an ACPs yield	713:725	an ACPs yield of 10.97%	713:735	The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained.					
31756485	2	0	from	size	431:434	arg1	combination					488:498	combination	488:498	combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	488:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	7	1	theme	drug	1266:1269	arg1	system					1280:1285	the gastroretentive drug delivery system	1246:1285	the gastroretentive drug delivery system	1246:1285	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	7	2	theme	potential	1209:1217	arg1	carrier					1224:1230	a potential drug carrier	1207:1230	a potential drug carrier	1207:1230	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	5	3	from	rate	930:933	arg1	mucosa					950:955	the gastric mucosa	938:955	the gastric mucosa of SA matrix	938:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	2	4	from	properties	454:463	arg1	combination					488:498	combination	488:498	combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	488:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	5	theme	blend	674:678	arg1	microspheres					680:691	SA-BSP blend microspheres	667:691	SA-BSP blend microspheres	667:691	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	6	6	theme	SA	1105:1106	arg1	microsphere					1108:1118	the SA microsphere	1101:1118	the SA microsphere	1101:1118	Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did.					
31756485	0	7	theme	Bletilla	113:120	arg1	polysaccharide					130:143	Bletilla striata polysaccharide	113:143	Bletilla striata polysaccharide	113:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	2	8	theme	gastric	597:603	arg1	retention					605:613	gastric retention	597:613	gastric retention	597:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	9	theme	SA-BSP	667:672	arg1	microspheres					680:691	SA-BSP blend microspheres	667:691	SA-BSP blend microspheres	667:691	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	6	10	theme	Mucoadhesion	1010:1021	arg1	tests					1023:1027	Mucoadhesion tests	1010:1027	Mucoadhesion tests	1010:1027	Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did.					
31756485	2	11	theme	swelling	538:545	arg1	adsorption					565:574	mucin adsorption	559:574	mucin adsorption	559:574	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	11	theme	swelling	538:545	arg1	properties					547:556	the swelling properties	534:556	the swelling properties	534:556	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	1	12	theme	gastrointestinal	352:367	arg1	tract					369:373	the gastrointestinal tract	348:373	the gastrointestinal tract	348:373	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	7	13	with	microspheres	1147:1158	arg1	mucoadhesion					1178:1189	the enhanced mucoadhesion	1165:1189	the enhanced mucoadhesion	1165:1189	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	4	14	theme	BSP	785:787	arg1	addition					773:780	The addition	769:780	The addition of BSP	769:787	The addition of BSP increased flexibility and reduced rigidity of SA microsphere.					
31756485	2	15	dep	properties	547:556	arg1	retention					605:613	gastric retention	597:613	gastric retention	597:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	5	16	from	ability	904:910	arg1	mucosa					950:955	the gastric mucosa	938:955	the gastric mucosa of SA matrix	938:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	1	17	from	striata	178:184	arg1	polysaccharide					149:162	polysaccharide	149:162	polysaccharide from Bletilla striata (BSP)	149:190	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	5	18	theme	mucin	887:891	arg1	ability					904:910	mucin adsorption ability	887:910	mucin adsorption ability	887:910	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	0	19	theme	striata	122:128	arg1	polysaccharide					130:143	Bletilla striata polysaccharide	113:143	Bletilla striata polysaccharide	113:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	2	20	theme	thermodynamic	440:452	arg1	properties					454:463	thermodynamic properties	440:463	thermodynamic properties	440:463	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	5	21	theme	adsorption	893:902	arg1	ability					904:910	mucin adsorption ability	887:910	mucin adsorption ability	887:910	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	2	22	from	microspheres	472:483	arg1	combination					488:498	combination	488:498	combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	488:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	5	23	theme	SA	960:961	arg1	matrix					963:968	SA matrix	960:968	SA matrix	960:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	0	24	theme	Mucoadhesive	0:11	arg1	improvement					13:23	Mucoadhesive improvement	0:23	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.	0:144	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	5	25	theme	matrix	963:968	arg1	mucosa					950:955	the gastric mucosa	938:955	the gastric mucosa of SA matrix	938:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	0	26	theme	alginate	28:35	arg1	microspheres					37:48	alginate microspheres	28:48	alginate microspheres	28:48	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	2	27	theme	microspheres	680:691	arg1	mucoadhesion					651:662	the mucoadhesion	647:662	the mucoadhesion of SA-BSP blend microspheres	647:691	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	0	28	with	blending	99:106	arg1	polysaccharide					130:143	Bletilla striata polysaccharide	113:143	Bletilla striata polysaccharide	113:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	2	29	theme	particle	422:429	arg1	size					431:434	particle size	422:434	particle size	422:434	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	1	30	theme	long	381:384	arg1	time					386:389	a long time	379:389	a long time	379:389	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	7	31	theme	delivery	1271:1278	arg1	system					1280:1285	the gastroretentive drug delivery system	1246:1285	the gastroretentive drug delivery system	1246:1285	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	5	32	theme	swelling	868:875	arg1	property					877:884	the swelling property	864:884	the swelling property	864:884	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	2	33	theme	mucin	559:563	arg1	adsorption					565:574	mucin adsorption	559:574	mucin adsorption	559:574	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	33	theme	mucin	559:563	arg1	properties					547:556	the swelling properties	534:556	the swelling properties	534:556	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	34	from	combination	488:498	arg1	properties					410:419	The morphological properties	392:419	The morphological properties	392:419	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	34	from	combination	488:498	arg1	size					431:434	particle size	422:434	particle size	422:434	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	34	from	combination	488:498	arg1	properties					454:463	thermodynamic properties	440:463	thermodynamic properties	440:463	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	7	35	theme	SA-BSP	1140:1145	arg1	microspheres					1147:1158	the SA-BSP microspheres	1136:1158	the SA-BSP microspheres with the enhanced mucoadhesion	1136:1189	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	0	36	theme	microspheres	37:48	arg1	improvement					13:23	Mucoadhesive improvement	0:23	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.	0:144	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	2	37	theme	microspheres	472:483	arg1	properties					410:419	The morphological properties	392:419	The morphological properties	392:419	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	37	theme	microspheres	472:483	arg1	size					431:434	particle size	422:434	particle size	422:434	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	37	theme	microspheres	472:483	arg1	properties					454:463	thermodynamic properties	440:463	thermodynamic properties	440:463	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	38	from	evaluations	519:529	arg1	adsorption					565:574	mucin adsorption	559:574	mucin adsorption	559:574	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	38	from	evaluations	519:529	arg1	properties					547:556	the swelling properties	534:556	the swelling properties	534:556	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	4	39	theme	microsphere	838:848	arg1	rigidity					823:830	rigidity	823:830	rigidity of SA microsphere	823:848	The addition of BSP increased flexibility and reduced rigidity of SA microsphere.					
31756485	5	40	from	property	877:884	arg1	mucosa					950:955	the gastric mucosa	938:955	the gastric mucosa of SA matrix	938:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	1	41	theme	sodium	241:246	arg1	SA					258:259	SA	258:259	SA	258:259	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	41	theme	sodium	241:246	arg1	microspheres					262:273	sodium alginate (SA) microspheres	241:273	sodium alginate (SA) microspheres	241:273	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	0	42	theme	gastroretentive	63:77	arg1	carrier					88:94	potential gastroretentive delivery carrier	53:94	potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide	53:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	7	43	theme	drug	1219:1222	arg1	carrier					1224:1230	a potential drug carrier	1207:1230	a potential drug carrier	1207:1230	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	2	44	theme	comprehensive	505:517	arg1	evaluations					519:529	comprehensive evaluations	505:529	comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	505:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	0	45	theme	potential	53:61	arg1	carrier					88:94	potential gastroretentive delivery carrier	53:94	potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide	53:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	1	46	theme	composite	313:321	arg1	microsphere					323:333	a composite microsphere	311:333	a composite microsphere to retain in the gastrointestinal tract for a long time	311:389	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	6	47	dep	showed	1029:1034	arg1	stayed					1060:1065	stayed	1060:1065	showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did	1029:1122	Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did.					
31756485	5	48	theme	gastric	942:948	arg1	mucosa					950:955	the gastric mucosa	938:955	the gastric mucosa of SA matrix	938:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
31756485	7	49	theme	enhanced	1169:1176	arg1	mucoadhesion					1178:1189	the enhanced mucoadhesion	1165:1189	the enhanced mucoadhesion	1165:1189	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	1	50	from	improvement	226:236	arg1	SA					258:259	SA	258:259	SA	258:259	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	50	from	improvement	226:236	arg1	microspheres					262:273	sodium alginate (SA) microspheres	241:273	sodium alginate (SA) microspheres	241:273	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	51	theme	alginate	248:255	arg1	SA					258:259	SA	258:259	SA	258:259	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	51	theme	alginate	248:255	arg1	microspheres					262:273	sodium alginate (SA) microspheres	241:273	sodium alginate (SA) microspheres	241:273	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	2	52	from	properties	410:419	arg1	combination					488:498	combination	488:498	combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	488:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	53	with	combination	488:498	arg1	evaluations					519:529	comprehensive evaluations	505:529	comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention	505:613	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	2	54	theme	morphological	396:408	arg1	properties					410:419	The morphological properties	392:419	The morphological properties	392:419	The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres.					
31756485	4	55	theme	SA	835:836	arg1	microsphere					838:848	SA microsphere	835:848	SA microsphere	835:848	The addition of BSP increased flexibility and reduced rigidity of SA microsphere.					
31756485	1	56	theme	mucoadhesive	213:224	arg1	improvement					226:236	mucoadhesive improvement	213:236	mucoadhesive improvement in sodium alginate (SA) microspheres	213:273	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	57	theme	Bletilla	169:176	arg1	striata					178:184	Bletilla striata	169:184	Bletilla striata (BSP)	169:190	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	1	57	theme	Bletilla	169:176	arg1	BSP					187:189	BSP	187:189	BSP	187:189	As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time.					
31756485	7	58	theme	gastroretentive	1250:1264	arg1	system					1280:1285	the gastroretentive drug delivery system	1246:1285	the gastroretentive drug delivery system	1246:1285	Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.					
31756485	6	59	theme	SA-BSP	1040:1045	arg1	microspheres					1047:1058	the SA-BSP microspheres	1036:1058	the SA-BSP microspheres	1036:1058	Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did.					
31756485	0	60	theme	delivery	79:86	arg1	carrier					88:94	potential gastroretentive delivery carrier	53:94	potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide	53:143	Mucoadhesive improvement of alginate microspheres as potential gastroretentive delivery carrier by blending with Bletilla striata polysaccharide.					
31756485	3	61	theme	prepared	718:725	arg1	discrete					745:752	discrete	745:752	discrete	745:752	Results showed that the prepared microspheres were discrete and spherical.					
31756485	3	61	theme	prepared	718:725	arg1	microspheres					727:738	the prepared microspheres	714:738	the prepared microspheres	714:738	Results showed that the prepared microspheres were discrete and spherical.					
31756485	5	62	theme	retention	920:928	arg1	rate					930:933	the retention rate	916:933	the retention rate on the gastric mucosa of SA matrix	916:968	Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP.					
29935270	8	0	theme	overweight	1191:1200	arg1	children					1202:1209	overweight children	1191:1209	overweight children	1191:1209	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	11	1	theme	levan	1724:1728	arg1	fermentation					1730:1741	levan fermentation	1724:1741	levan fermentation	1724:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	3	2	theme	reference	702:710	arg1	bacterium					712:720	a reference bacterium	700:720	a reference bacterium for colon microbiota	700:741	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	10	3	theme	specific	1448:1455	arg1	promotion					1457:1465	the specific promotion	1444:1465	the specific promotion of Bacteroides and Lachnospiraceae by levans	1444:1510	In the adult pool, the specific promotion of Bacteroides and Lachnospiraceae by levans was disclosed.					
29935270	3	4	theme	fecal	559:563	arg1	samples					565:571	pooled fecal samples	552:571	pooled fecal samples	552:571	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	8	5	theme	lactic	1224:1229	arg1	acid					1231:1234	lactic acid	1224:1234	lactic acid	1224:1234	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	11	6	theme	production	1705:1714	arg1	rate					1716:1719	the highest maximum heat production rate	1680:1719	the highest maximum heat production rate at levan fermentation	1680:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	8	7	theme	major	1243:1247	arg1	product					1262:1268	the major fermentation product	1239:1268	the major fermentation product on short saccharides	1239:1289	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	13	8	theme	non-digestible	2062:2075	arg1	oligo-					2077:2082	non-digestible oligo-	2062:2082	non-digestible oligo-	2062:2082	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	9	9	theme	Acetic	1292:1297	arg1	acids					1311:1315	Acetic and butyric acids	1292:1315	Acetic and butyric acids	1292:1315	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	8	10	theme	short	1273:1277	arg1	saccharides					1279:1289	short saccharides	1273:1289	short saccharides	1273:1289	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	9	11	theme	butyric	1303:1309	arg1	acids					1311:1315	Acetic and butyric acids	1292:1315	Acetic and butyric acids	1292:1315	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	7	12	theme	pool-specific	1016:1028	arg1	responses					1048:1056	Several pool-specific substrate-related responses	1008:1056	Several pool-specific substrate-related responses to raffinose and melibiose	1008:1083	Several pool-specific substrate-related responses to raffinose and melibiose were revealed.					
29935270	11	13	theme	normal-weight	1548:1560	arg1	children					1562:1569	normal-weight children	1548:1569	normal-weight children	1548:1569	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	12	14	link	raffinose-derived	1755:1771	arg1	oligosaccharides					1773:1788	raffinose-derived oligosaccharides	1755:1788	raffinose-derived oligosaccharides	1755:1788	Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron.					
29935270	3	15	theme	adult	629:633	arg1	volunteers					635:644	healthy adult volunteers	621:644	healthy adult volunteers	621:644	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	13	16	dep	manners	2051:2057	arg1	manners					2051:2057	specific manners	2042:2057	specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans	2042:2171	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	16	dep	manners	2051:2057	arg1	levans					2166:2171	levans	2166:2171	levans	2166:2171	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	16	dep	manners	2051:2057	arg1	melibiose					2116:2124	melibiose	2116:2124	melibiose	2116:2124	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	16	dep	manners	2051:2057	arg1	oligosaccharides					2145:2160	raffinose-derived oligosaccharides	2127:2160	raffinose-derived oligosaccharides	2127:2160	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	16	dep	manners	2051:2057	arg1	raffinose					2105:2113	raffinose	2105:2113	raffinose	2105:2113	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	8	17	theme	children	1202:1209	arg1	pool					1183:1186	the pool	1179:1186	the pool of overweight children resulting in lactic acid	1179:1234	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	0	18	from	metabolism	16:25	arg1	overweight					63:72	overweight	63:72	overweight	63:72	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	18	from	metabolism	16:25	arg1	normal					52:57	normal	52:57	normal	52:57	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	14	19	theme	microbiota	2252:2261	arg1	balance					2235:2241	a healthy balance	2225:2241	a healthy balance of colon microbiota	2225:2261	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	5	20	theme	rDNA	909:912	arg1	sequencing					914:923	rDNA sequencing	909:923	rDNA sequencing	909:923	Taxonomic profiles of the microbiota were assessed by 16S rDNA sequencing.					
29935270	8	21	from	enriched	1167:1174	arg1	pool					1183:1186	the pool	1179:1186	the pool of overweight children resulting in lactic acid	1179:1234	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	0	22	from	Composition	0:10	arg1	overweight					63:72	overweight	63:72	overweight	63:72	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	22	from	Composition	0:10	arg1	normal					52:57	normal	52:57	normal	52:57	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	4	23	theme	heat	781:784	arg1	curves					796:801	the heat evolution curves	777:801	the heat evolution curves	777:801	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	13	24	theme	different	1999:2007	arg1	compositions					2009:2020	different compositions	1999:2020	different compositions	1999:2020	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	9	25	theme	normal-weight	1355:1367	arg1	pool					1369:1372	the normal-weight pool	1351:1372	the normal-weight pool coinciding with the enrichment of Catenibacterium	1351:1422	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	0	26	link	raffinose-derived	139:155	arg1	fructans					157:164	raffinose-derived fructans	139:164	raffinose-derived fructans	139:164	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	6	27	theme	fecal	995:999	arg1	pools					1001:1005	all fecal pools	991:1005	all fecal pools	991:1005	Raffinose and melibiose promoted the growth of bifidobacteria in all fecal pools.					
29935270	13	28	contain	have	1994:1997	arg2	compositions					2009:2020	different compositions	1999:2020	different compositions	1999:2020	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	28	contain	have	1994:1997	arg1	microbiota					1949:1958	fecal microbiota	1943:1958	fecal microbiota of normal and overweight children	1943:1992	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	14	29	theme	healthy	2227:2233	arg1	balance					2235:2241	a healthy balance	2225:2241	a healthy balance of colon microbiota	2225:2261	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	2	30	theme	polysaccharides	401:415	arg1	raffinose					355:363	raffinose	355:363	raffinose	355:363	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	30	theme	polysaccharides	401:415	arg1	levan					496:500	levan	496:500	levan from timothy grass	496:519	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	30	theme	polysaccharides	401:415	arg1	substrates					339:348	The five tested substrates	323:348	The five tested substrates	323:348	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	30	theme	polysaccharides	401:415	arg1	melibiose					366:374	melibiose	366:374	melibiose	366:374	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	30	theme	polysaccharides	401:415	arg1	mixture					379:385	a mixture	377:385	a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose	377:489	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	13	31	theme	normal	1963:1968	arg1	microbiota					1949:1958	fecal microbiota	1943:1958	fecal microbiota of normal and overweight children	1943:1992	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	11	32	from	fermentation	1730:1741	arg1	rate					1716:1719	the highest maximum heat production rate	1680:1719	the highest maximum heat production rate at levan fermentation	1680:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	2	33	theme	oligo-	390:395	arg1	raffinose					355:363	raffinose	355:363	raffinose	355:363	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	33	theme	oligo-	390:395	arg1	levan					496:500	levan	496:500	levan from timothy grass	496:519	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	33	theme	oligo-	390:395	arg1	substrates					339:348	The five tested substrates	323:348	The five tested substrates	323:348	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	33	theme	oligo-	390:395	arg1	melibiose					366:374	melibiose	366:374	melibiose	366:374	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	33	theme	oligo-	390:395	arg1	mixture					379:385	a mixture	377:385	a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose	377:489	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	8	34	theme	Lactate-producing	1100:1116	arg1	bacteria					1118:1125	Lactate-producing bacteria	1100:1125	Lactate-producing bacteria (Streptococcus and Enterococcus)	1100:1158	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	14	35	theme	saccharides	2202:2212	arg1	potential					2178:2186	The potential	2174:2186	The potential of the tested saccharides to support a healthy balance of colon microbiota	2174:2261	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	3	36	from	children	606:613	arg1	samples					565:571	pooled fecal samples	552:571	pooled fecal samples	552:571	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	1	37	theme	isothermal	294:303	arg1	microcalorimetry					305:320	isothermal microcalorimetry	294:320	isothermal microcalorimetry	294:320	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	4	38	theme	organic	826:832	arg1	acids					834:838	organic acids	826:838	organic acids	826:838	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	13	39	theme	fecal	1943:1947	arg1	microbiota					1949:1958	fecal microbiota	1943:1958	fecal microbiota of normal and overweight children	1943:1992	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	0	40	theme	fecal	30:34	arg1	microbiota					36:45	fecal microbiota	30:45	fecal microbiota from normal and overweight children	30:81	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	8	41	from	pool	1183:1186	arg1	enriched					1167:1174	enriched	1167:1174	enriched	1167:1174	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	13	42	dep	normal	1963:1968	arg1	children					1985:1992	children	1985:1992	children	1985:1992	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	3	43	theme	pure	652:655	arg1	culture					657:663	a pure culture	650:663	a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota	650:741	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	13	44	theme	raffinose-derived	2127:2143	arg1	oligosaccharides					2145:2160	raffinose-derived oligosaccharides	2127:2160	raffinose-derived oligosaccharides	2127:2160	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	6	45	from	growth	963:968	arg1	pools					1001:1005	all fecal pools	991:1005	all fecal pools	991:1005	Raffinose and melibiose promoted the growth of bifidobacteria in all fecal pools.					
29935270	12	46	theme	thetaiotaomicron	1882:1897	arg1	culture					1859:1865	a pure culture	1852:1865	a pure culture of Bacteroides thetaiotaomicron	1852:1897	Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron.					
29935270	8	47	dep	bacteria	1118:1125	arg1	Enterococcus					1146:1157	Enterococcus	1146:1157	Enterococcus	1146:1157	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	8	47	dep	bacteria	1118:1125	arg1	Streptococcus					1128:1140	Streptococcus	1128:1140	Streptococcus	1128:1140	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	3	48	theme	thetaiotaomicron	680:695	arg1	culture					657:663	a pure culture	650:663	a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota	650:741	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	3	48	theme	thetaiotaomicron	680:695	arg1	volunteers					635:644	healthy adult volunteers	621:644	healthy adult volunteers	621:644	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	11	49	theme	heat	1700:1703	arg1	rate					1716:1719	the highest maximum heat production rate	1680:1719	the highest maximum heat production rate at levan fermentation	1680:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	1	50	theme	oligo-	240:245	arg1	metabolism					211:220	the metabolism	207:220	the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota	207:285	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	9	51	from	prevalent	1322:1330	arg1	pool					1369:1372	the normal-weight pool	1351:1372	the normal-weight pool coinciding with the enrichment of Catenibacterium	1351:1422	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	3	52	theme	colon	726:730	arg1	microbiota					732:741	colon microbiota	726:741	colon microbiota	726:741	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	11	53	theme	highest	1684:1690	arg1	rate					1716:1719	the highest maximum heat production rate	1680:1719	the highest maximum heat production rate at levan fermentation	1680:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	1	54	theme	polysaccharides	251:265	arg1	metabolism					211:220	the metabolism	207:220	the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota	207:285	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	12	55	theme	pure	1854:1857	arg1	culture					1859:1865	a pure culture	1852:1865	a pure culture of Bacteroides thetaiotaomicron	1852:1897	Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron.					
29935270	13	56	theme	specific	2042:2049	arg1	manners					2051:2057	specific manners	2042:2057	specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans	2042:2171	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	56	theme	specific	2042:2049	arg1	melibiose					2116:2124	melibiose	2116:2124	melibiose	2116:2124	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	13	56	theme	specific	2042:2049	arg1	raffinose					2105:2113	raffinose	2105:2113	raffinose	2105:2113	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	11	57	theme	Senegalimassilia	1604:1619	arg1	growth					1594:1599	the growth	1590:1599	the growth of Senegalimassilia and Lachnoclostridium	1590:1641	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	3	58	theme	pooled	552:557	arg1	samples					565:571	pooled fecal samples	552:571	pooled fecal samples	552:571	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	1	59	theme	fecal	270:274	arg1	microbiota					276:285	fecal microbiota	270:285	fecal microbiota	270:285	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	2	60	theme	tested	332:337	arg1	raffinose					355:363	raffinose	355:363	raffinose	355:363	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	60	theme	tested	332:337	arg1	substrates					339:348	The five tested substrates	323:348	The five tested substrates	323:348	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	3	61	from	culture	657:663	arg1	samples					565:571	pooled fecal samples	552:571	pooled fecal samples	552:571	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	5	62	theme	microbiota	877:886	arg1	profiles					861:868	Taxonomic profiles	851:868	Taxonomic profiles of the microbiota	851:886	Taxonomic profiles of the microbiota were assessed by 16S rDNA sequencing.					
29935270	8	63	from	product	1262:1268	arg1	saccharides					1279:1289	short saccharides	1273:1289	short saccharides	1273:1289	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	4	64	theme	gases	844:848	arg1	production					812:821	the production	808:821	the production of organic acids and gases	808:848	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	4	64	theme	gases	844:848	arg1	curves					796:801	the heat evolution curves	777:801	the heat evolution curves	777:801	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	10	65	theme	Bacteroides	1470:1480	arg1	promotion					1457:1465	the specific promotion	1444:1465	the specific promotion of Bacteroides and Lachnospiraceae by levans	1444:1510	In the adult pool, the specific promotion of Bacteroides and Lachnospiraceae by levans was disclosed.					
29935270	3	66	theme	overweight	578:587	arg1	children					606:613	overweight or normal-weight children	578:613	overweight or normal-weight children	578:613	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	1	67	theme	study	182:186	arg1	aim					171:173	The aim	167:173	The aim of the study	167:186	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	11	68	theme	Lachnoclostridium	1625:1641	arg1	growth					1594:1599	the growth	1590:1599	the growth of Senegalimassilia and Lachnoclostridium	1590:1641	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	9	69	from	pool	1369:1372	arg1	prevalent					1322:1330	prevalent	1322:1330	prevalent	1322:1330	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	6	70	theme	bifidobacteria	973:986	arg1	growth					963:968	the growth	959:968	the growth of bifidobacteria in all fecal pools	959:1005	Raffinose and melibiose promoted the growth of bifidobacteria in all fecal pools.					
29935270	0	71	theme	raffinose-derived	139:155	arg1	fructans					157:164	raffinose-derived fructans	139:164	raffinose-derived fructans	139:164	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	5	72	theme	Taxonomic	851:859	arg1	profiles					861:868	Taxonomic profiles	851:868	Taxonomic profiles of the microbiota	851:886	Taxonomic profiles of the microbiota were assessed by 16S rDNA sequencing.					
29935270	8	73	theme	fermentation	1249:1260	arg1	product					1262:1268	the major fermentation product	1239:1268	the major fermentation product on short saccharides	1239:1289	Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides.					
29935270	2	74	from	grass	515:519	arg1	raffinose					355:363	raffinose	355:363	raffinose	355:363	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	74	from	grass	515:519	arg1	levan					496:500	levan	496:500	levan from timothy grass	496:519	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	74	from	grass	515:519	arg1	substrates					339:348	The five tested substrates	323:348	The five tested substrates	323:348	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	74	from	grass	515:519	arg1	melibiose					366:374	melibiose	366:374	melibiose	366:374	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	2	74	from	grass	515:519	arg1	mixture					379:385	a mixture	377:385	a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose	377:489	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	14	75	theme	further	2272:2278	arg1	studies					2280:2286	further studies	2272:2286	further studies	2272:2286	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	3	76	theme	normal-weight	592:604	arg1	children					606:613	overweight or normal-weight children	578:613	overweight or normal-weight children	578:613	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	9	77	from	fermentation	1335:1346	arg1	prevalent					1322:1330	prevalent	1322:1330	prevalent	1322:1330	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	7	78	theme	substrate-related	1030:1046	arg1	responses					1048:1056	Several pool-specific substrate-related responses	1008:1056	Several pool-specific substrate-related responses to raffinose and melibiose	1008:1083	Several pool-specific substrate-related responses to raffinose and melibiose were revealed.					
29935270	4	79	dep	analyzed	759:766	arg1	based					768:772	based	768:772	was analyzed based on the heat evolution curves, and the production of organic acids and gases	755:848	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	11	80	theme	fecal	1534:1538	arg1	pool					1540:1543	the fecal pool	1530:1543	the fecal pool of normal-weight children	1530:1569	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	3	81	theme	healthy	621:627	arg1	volunteers					635:644	healthy adult volunteers	621:644	healthy adult volunteers	621:644	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	7	82	theme	Several	1008:1014	arg1	responses					1048:1056	Several pool-specific substrate-related responses	1008:1056	Several pool-specific substrate-related responses to raffinose and melibiose	1008:1083	Several pool-specific substrate-related responses to raffinose and melibiose were revealed.					
29935270	2	83	theme	timothy	507:513	arg1	grass					515:519	timothy grass	507:519	timothy grass	507:519	The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass.					
29935270	13	84	theme	overweight	1974:1983	arg1	microbiota					1949:1958	fecal microbiota	1943:1958	fecal microbiota of normal and overweight children	1943:1992	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	12	85	theme	raffinose-derived	1755:1771	arg1	oligosaccharides					1773:1788	raffinose-derived oligosaccharides	1755:1788	raffinose-derived oligosaccharides	1755:1788	Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron.					
29935270	14	86	theme	colon	2246:2250	arg1	microbiota					2252:2261	colon microbiota	2246:2261	colon microbiota	2246:2261	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	11	87	theme	children	1562:1569	arg1	pool					1540:1543	the fecal pool	1530:1543	the fecal pool of normal-weight children	1530:1569	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	10	88	theme	adult	1432:1436	arg1	pool					1438:1441	the adult pool	1428:1441	the adult pool	1428:1441	In the adult pool, the specific promotion of Bacteroides and Lachnospiraceae by levans was disclosed.					
29935270	0	89	from	normal	52:57	arg1	metabolism					16:25	metabolism	16:25	metabolism	16:25	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	89	from	normal	52:57	arg1	microbiota					36:45	fecal microbiota	30:45	fecal microbiota from normal and overweight children	30:81	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	89	from	normal	52:57	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	10	90	theme	Lachnospiraceae	1486:1500	arg1	promotion					1457:1465	the specific promotion	1444:1465	the specific promotion of Bacteroides and Lachnospiraceae by levans	1444:1510	In the adult pool, the specific promotion of Bacteroides and Lachnospiraceae by levans was disclosed.					
29935270	4	91	theme	evolution	786:794	arg1	curves					796:801	the heat evolution curves	777:801	the heat evolution curves	777:801	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	3	92	from	volunteers	635:644	arg1	samples					565:571	pooled fecal samples	552:571	pooled fecal samples	552:571	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	13	93	theme	main	1904:1907	arg1	conclusion					1909:1918	The main conclusion	1900:1918	The main conclusion from the study	1900:1933	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	14	94	theme	tested	2195:2200	arg1	saccharides					2202:2212	the tested saccharides	2191:2212	the tested saccharides	2191:2212	The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.					
29935270	0	95	theme	microbiota	36:45	arg1	metabolism					16:25	metabolism	16:25	metabolism	16:25	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	95	theme	microbiota	36:45	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	9	96	theme	Catenibacterium	1408:1422	arg1	enrichment					1394:1403	the enrichment	1390:1403	the enrichment of Catenibacterium	1390:1422	Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium.					
29935270	0	97	dep	normal	52:57	arg1	children					74:81	children	74:81	children	74:81	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	13	98	from	study	1929:1933	arg1	conclusion					1909:1918	The main conclusion	1900:1918	The main conclusion from the study	1900:1933	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	4	99	theme	acids	834:838	arg1	production					812:821	the production	808:821	the production of organic acids and gases	808:848	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	4	99	theme	acids	834:838	arg1	curves					796:801	the heat evolution curves	777:801	the heat evolution curves	777:801	The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases.					
29935270	11	100	theme	particular	1652:1661	arg1	pool					1663:1666	this particular pool	1647:1666	this particular pool	1647:1666	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	0	101	from	overweight	63:72	arg1	metabolism					16:25	metabolism	16:25	metabolism	16:25	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	101	from	overweight	63:72	arg1	microbiota					36:45	fecal microbiota	30:45	fecal microbiota from normal and overweight children	30:81	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	0	101	from	overweight	63:72	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans.					
29935270	13	102	link	raffinose-derived	2127:2143	arg1	oligosaccharides					2145:2160	raffinose-derived oligosaccharides	2127:2160	raffinose-derived oligosaccharides	2127:2160	The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans.					
29935270	3	103	theme	Bacteroides	668:678	arg1	thetaiotaomicron					680:695	Bacteroides thetaiotaomicron	668:695	Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota	668:741	Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota.					
29935270	11	104	theme	maximum	1692:1698	arg1	rate					1716:1719	the highest maximum heat production rate	1680:1719	the highest maximum heat production rate at levan fermentation	1680:1741	In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation.					
29935270	1	105	theme	non-digestible	225:238	arg1	oligo-					240:245	non-digestible oligo-	225:245	non-digestible oligo-	225:245	The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry.					
29935270	12	106	theme	Bacteroides	1870:1880	arg1	thetaiotaomicron					1882:1897	Bacteroides thetaiotaomicron	1870:1897	Bacteroides thetaiotaomicron	1870:1897	Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron.					
30036623	4	0	theme	natural	906:912	arg1	antioxidant					914:924	natural antioxidant	906:924	natural antioxidant	906:924	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	2	1	theme	scavenging	585:594	arg1	activity					596:603	moderate hydroxyl radical scavenging activity	559:603	moderate hydroxyl radical scavenging activity	559:603	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	1	2	theme	P.	237:238	arg1	tricuspidata					240:251	P. tricuspidata	237:251	P. tricuspidata	237:251	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	2	3	theme	comparable	488:497	arg1	activity					478:485	strong superoxide radical activity	452:485	strong superoxide radical activity (comparable to BHT)	452:505	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	3	4	theme	PTP-4	673:677	arg1	structure					660:668	the chemical structure	647:668	the chemical structure of PTP-4	647:677	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	5	theme	radical	577:583	arg1	activity					596:603	moderate hydroxyl radical scavenging activity	559:603	moderate hydroxyl radical scavenging activity	559:603	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	1	6	theme	tricuspidata	240:251	arg1	stems					228:232	the stems	224:232	the stems of P. tricuspidata	224:251	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	0	7	theme	tricuspidata	114:125	arg1	stems					90:94	the stems	86:94	the stems of Parthenocissus tricuspidata	86:125	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	2	8	theme	hydroxyl	568:575	arg1	activity					596:603	moderate hydroxyl radical scavenging activity	559:603	moderate hydroxyl radical scavenging activity	559:603	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	3	9	theme	arabinose	771:779	arg1	composition					752:762	its mainly composition	741:762	its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose	741:828	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	0	10	theme	Parthenocissus	99:112	arg1	tricuspidata					114:125	Parthenocissus tricuspidata	99:125	Parthenocissus tricuspidata	99:125	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	3	11	theme	GC	702:703	arg1	spectra					721:727	FT-IR, GC, 1H and 13C NMR spectra	695:727	spectra	721:727	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	12	theme	moderate	559:566	arg1	activity					596:603	moderate hydroxyl radical scavenging activity	559:603	moderate hydroxyl radical scavenging activity	559:603	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	1	13	theme	anion	256:260	arg1	chromatography					271:284	anion exchange chromatography	256:284	anion exchange chromatography	256:284	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	2	14	theme	high	508:511	arg1	activity					526:533	high DPPH radical activity	508:533	high DPPH radical activity (78.53% at 1250 μg/mL)	508:556	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	15	theme	power	618:622	arg1	activity					624:631	reducing power activity	609:631	reducing power activity	609:631	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	16	theme	superoxide	459:468	arg1	activity					478:485	strong superoxide radical activity	452:485	strong superoxide radical activity (comparable to BHT)	452:505	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	3	17	theme	FT-IR	695:699	arg1	spectra					721:727	FT-IR, GC, 1H and 13C NMR spectra	695:727	spectra	721:727	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	18	theme	reducing	609:616	arg1	activity					624:631	reducing power activity	609:631	reducing power activity	609:631	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	19	theme	strong	452:457	arg1	activity					478:485	strong superoxide radical activity	452:485	strong superoxide radical activity (comparable to BHT)	452:505	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	3	20	theme	13C	713:715	arg1	spectra					721:727	FT-IR, GC, 1H and 13C NMR spectra	695:727	spectra	721:727	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	21	theme	antioxidant	310:320	arg1	activities					322:331	The antioxidant activities	306:331	The antioxidant activities of four PTPs	306:344	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	22	theme	antioxidant	386:396	arg1	activities					398:407	different antioxidant activities	376:407	different antioxidant activities	376:407	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	0	23	theme	Chemical	0:7	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	1	24	theme	exchange	262:269	arg1	chromatography					271:284	anion exchange chromatography	256:284	anion exchange chromatography	256:284	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	4	25	used	used	875:878	arg2	source					895:900	a potential source	883:900	a potential source for natural antioxidant	883:924	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	4	25	used	used	875:878	arg2	stems					841:845	the stems	837:845	the stems of P. tricuspidata	837:864	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	2	26	theme	different	376:384	arg1	activities					398:407	different antioxidant activities	376:407	different antioxidant activities	376:407	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	0	27	theme	antioxidant	30:40	arg1	activities					42:51	antioxidant activities	30:51	antioxidant activities	30:51	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	3	28	theme	NMR	717:719	arg1	spectra					721:727	FT-IR, GC, 1H and 13C NMR spectra	695:727	spectra	721:727	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	4	29	theme	P.	850:851	arg1	tricuspidata					853:864	P. tricuspidata	850:864	P. tricuspidata	850:864	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	3	30	theme	1H	706:707	arg1	spectra					721:727	FT-IR, GC, 1H and 13C NMR spectra	695:727	spectra	721:727	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	1	31	theme	gel	290:292	arg1	filtration					294:303	gel filtration	290:303	gel filtration	290:303	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	2	32	from	1250 μg/mL	546:555	arg1	%					541:541	78.53%	536:541	78.53% at 1250 μg/mL	536:555	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	1	33	theme	water	204:208	arg1	extraction					210:219	the water extraction	200:219	the water extraction of the stems of P. tricuspidata	200:251	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	4	34	theme	tricuspidata	853:864	arg1	source					895:900	a potential source	883:900	a potential source for natural antioxidant	883:924	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	4	34	theme	tricuspidata	853:864	arg1	stems					841:845	the stems	837:845	the stems of P. tricuspidata	837:864	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	2	35	theme	radical	470:476	arg1	activity					478:485	strong superoxide radical activity	452:485	strong superoxide radical activity (comparable to BHT)	452:505	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	36	theme	radical	518:524	arg1	activity					526:533	high DPPH radical activity	508:533	high DPPH radical activity (78.53% at 1250 μg/mL)	508:556	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	2	37	theme	PTPs	341:344	arg1	activities					322:331	The antioxidant activities	306:331	The antioxidant activities of four PTPs	306:344	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	0	38	theme	polysaccharides	56:70	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	0	38	theme	polysaccharides	56:70	arg1	activities					42:51	antioxidant activities	30:51	antioxidant activities	30:51	Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata.					
30036623	3	39	theme	glucuronic	801:810	arg1	acid					812:815	glucuronic acid	801:815	glucuronic acid	801:815	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	3	40	theme	acid	812:815	arg1	composition					752:762	its mainly composition	741:762	its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose	741:828	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	3	41	theme	xylose	782:787	arg1	composition					752:762	its mainly composition	741:762	its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose	741:828	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	3	42	theme	galactose	790:798	arg1	composition					752:762	its mainly composition	741:762	its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose	741:828	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	3	43	theme	chemical	651:658	arg1	structure					660:668	the chemical structure	647:668	the chemical structure of PTP-4	647:677	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	44	dep	activity	526:533	arg1	%					541:541	78.53%	536:541	78.53% at 1250 μg/mL	536:555	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
30036623	1	45	theme	stems	228:232	arg1	extraction					210:219	the water extraction	200:219	the water extraction of the stems of P. tricuspidata	200:251	Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration.					
30036623	4	46	theme	potential	885:893	arg1	source					895:900	a potential source	883:900	a potential source for natural antioxidant	883:924	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	4	46	theme	potential	885:893	arg1	stems					841:845	the stems	837:845	the stems of P. tricuspidata	837:864	Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.					
30036623	3	47	theme	mannose	822:828	arg1	composition					752:762	its mainly composition	741:762	its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose	741:828	Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, 1H and 13C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose.					
30036623	2	48	theme	DPPH	513:516	arg1	activity					526:533	high DPPH radical activity	508:533	high DPPH radical activity (78.53% at 1250 μg/mL)	508:556	The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity.					
31707031	0	0	theme	Flammulina	106:115	arg1	velutipes					117:125	Flammulina velutipes	106:125	Flammulina velutipes	106:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	5	1	theme	substandard	928:938	arg1	samples					940:946	substandard samples	928:946	substandard samples	928:946	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	7	2	theme	inhibitory	1195:1204	arg1	relationship					1215:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	6	3	theme	F.	1106:1107	arg1	velutipes					1109:1117	white and yellow F. velutipes	1089:1117	white and yellow F. velutipes polysaccharides in HPLC fingerprints	1089:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	1	4	theme	important	173:181	arg1	compounds					193:201	the most important bioactive compounds	164:201	the most important bioactive compounds in Flammulina velutipes	164:225	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	6	5	theme	velutipes	1109:1117	arg1	discrimination					1071:1084	discrimination	1071:1084	discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints	1071:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	7	6	theme	activity	1206:1213	arg1	relationship					1215:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	1	7	theme	bioactive	183:191	arg1	compounds					193:201	the most important bioactive compounds	164:201	the most important bioactive compounds in Flammulina velutipes	164:225	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	4	8	theme	inhibitory	603:612	arg1	activities					614:623	The inhibitory activities	599:623	The inhibitory activities of FVPs against HepG2	599:645	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	5	9	theme	principal	777:785	arg1	analysis					797:804	principal component analysis	777:804	The principal component analysis (PCA) scores	773:817	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	5	9	theme	principal	777:785	arg1	PCA					807:809	PCA	807:809	PCA	807:809	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	8	10	theme	products	1613:1620	arg1	control					1573:1579	the quality control	1561:1579	the quality control of F. velutipes and its related products	1561:1620	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	7	11	theme	HPLC	1171:1174	arg1	relationship					1215:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	6	12	from	polysaccharides	1119:1133	arg1	fingerprints					1143:1154	HPLC fingerprints	1138:1154	HPLC fingerprints	1138:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	2	13	theme	F.	323:324	arg1	polysaccharides					336:350	F. velutipes polysaccharides	323:350	F. velutipes polysaccharides (FVPs) from different sources	323:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	2	13	theme	F.	323:324	arg1	FVPs					353:356	FVPs	353:356	FVPs	353:356	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	4	14	theme	regression	697:706	arg1	analysis					714:721	multiple linear regression (MLR) analysis	681:721	multiple linear regression (MLR) analysis	681:721	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	4	15	theme	FVPs	628:631	arg1	activities					614:623	The inhibitory activities	599:623	The inhibitory activities of FVPs against HepG2	599:645	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	5	16	theme	component	787:795	arg1	analysis					797:804	principal component analysis	777:804	The principal component analysis (PCA) scores	773:817	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	5	16	theme	component	787:795	arg1	PCA					807:809	PCA	807:809	PCA	807:809	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	0	17	from	assessment	71:80	arg1	velutipes					117:125	Flammulina velutipes	106:125	Flammulina velutipes	106:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	7	18	theme	fingerprint-growth	1176:1193	arg1	relationship					1215:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	the HPLC fingerprint-growth inhibitory activity relationship	1167:1226	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	2	19	from	sources	374:380	arg1	polysaccharides					336:350	F. velutipes polysaccharides	323:350	F. velutipes polysaccharides (FVPs) from different sources	323:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	2	19	from	sources	374:380	arg1	FVPs					353:356	FVPs	353:356	FVPs	353:356	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	5	20	theme	analysis	797:804	arg1	scores					812:817	The principal component analysis (PCA) scores	773:817	The principal component analysis (PCA) scores	773:817	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	5	21	theme	F.	890:891	arg1	velutipes					893:901	different F. velutipes	880:901	different F. velutipes	880:901	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	6	22	dep	velutipes	1109:1117	arg1	polysaccharides					1119:1133	polysaccharides	1119:1133	white and yellow F. velutipes polysaccharides in HPLC fingerprints	1089:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	3	23	theme	electron	456:463	arg1	SEM					477:479	SEM	477:479	SEM	477:479	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	23	theme	electron	456:463	arg1	microscopy					465:474	scanning electron microscopy	447:474	scanning electron microscopy (SEM)	447:480	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	1	24	theme	compounds	193:201	arg1	one					157:159	one	157:159	one	157:159	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	1	24	theme	compounds	193:201	arg1	compounds					193:201	the most important bioactive compounds	164:201	the most important bioactive compounds in Flammulina velutipes	164:225	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	1	24	theme	compounds	193:201	arg1	Polysaccharides					128:142	Polysaccharides	128:142	Polysaccharides	128:142	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	3	25	theme	fingerprint	409:419	arg1	method					430:435	a multiple fingerprint analysis method	398:435	a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC)	398:580	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	4	26	theme	linear	690:695	arg1	analysis					714:721	multiple linear regression (MLR) analysis	681:721	multiple linear regression (MLR) analysis	681:721	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	3	27	theme	multiple	400:407	arg1	method					430:435	a multiple fingerprint analysis method	398:435	a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC)	398:580	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	6	28	theme	glucuronic	1019:1028	arg1	acid					1030:1033	The glucuronic acid	1015:1033	The glucuronic acid	1015:1033	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	6	28	theme	glucuronic	1019:1028	arg1	marker					1060:1065	a marker	1058:1065	a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints	1058:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	7	29	theme	inhibitory	1318:1327	arg1	activity					1329:1336	the HepG2 growth inhibitory activity	1301:1336	the HepG2 growth inhibitory activity	1301:1336	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	1	30	theme	Flammulina	206:215	arg1	velutipes					217:225	Flammulina velutipes	206:225	Flammulina velutipes	206:225	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	0	31	theme	Multiple	0:7	arg1	fingerprint					9:19	Multiple fingerprint	0:19	Multiple fingerprint	0:19	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	8	32	theme	velutipes	1587:1595	arg1	control					1573:1579	the quality control	1561:1579	the quality control of F. velutipes and its related products	1561:1620	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	7	33	from	sources	1456:1462	arg1	FVPs					1436:1439	FVPs	1436:1439	FVPs from different sources	1436:1462	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	0	34	theme	fingerprint-activity	25:44	arg1	relationship					46:57	fingerprint-activity relationship	25:57	fingerprint-activity relationship	25:57	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	6	35	theme	yellow	1099:1104	arg1	velutipes					1109:1117	white and yellow F. velutipes	1089:1117	white and yellow F. velutipes polysaccharides in HPLC fingerprints	1089:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	3	36	theme	Fourier-transform	483:499	arg1	FT-IR					524:528	FT-IR	524:528	FT-IR	524:528	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	36	theme	Fourier-transform	483:499	arg1	spectroscopy					510:521	Fourier-transform infrared spectroscopy	483:521	Fourier-transform infrared spectroscopy (FT-IR)	483:529	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	2	37	theme	velutipes	326:334	arg1	polysaccharides					336:350	F. velutipes polysaccharides	323:350	F. velutipes polysaccharides (FVPs) from different sources	323:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	2	37	theme	velutipes	326:334	arg1	FVPs					353:356	FVPs	353:356	FVPs	353:356	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	3	38	theme	infrared	501:508	arg1	FT-IR					524:528	FT-IR	524:528	FT-IR	524:528	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	38	theme	infrared	501:508	arg1	spectroscopy					510:521	Fourier-transform infrared spectroscopy	483:521	Fourier-transform infrared spectroscopy (FT-IR)	483:529	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	4	39	theme	fingerprint-activity	738:757	arg1	relationship					759:770	fingerprint-activity relationship	738:770	fingerprint-activity relationship	738:770	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	5	40	theme	velutipes	893:901	arg1	batches					869:875	20 batches	866:875	20 batches of different F. velutipes	866:901	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	8	41	theme	fingerprint-activity	1484:1503	arg1	method					1518:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	4	42	theme	multiple	681:688	arg1	analysis					714:721	multiple linear regression (MLR) analysis	681:721	multiple linear regression (MLR) analysis	681:721	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	3	43	theme	scanning	447:454	arg1	SEM					477:479	SEM	477:479	SEM	477:479	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	43	theme	scanning	447:454	arg1	microscopy					465:474	scanning electron microscopy	447:474	scanning electron microscopy (SEM)	447:480	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	7	44	theme	monosaccharides	1245:1259	arg1	composition					1261:1271	monosaccharides composition	1245:1271	monosaccharides composition	1245:1271	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	8	45	theme	suggested	1469:1477	arg1	method					1518:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	2	46	theme	accurate	249:256	arg1	method					287:292	no accurate and comprehensive assessment method	246:292	no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources	246:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	7	47	theme	activity-associated	1343:1361	arg1	markers					1363:1369	activity-associated markers	1343:1369	activity-associated markers (mannose, rhamnose, xylose, and galactose)	1343:1412	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	47	theme	activity-associated	1343:1361	arg1	xylose					1391:1396	xylose	1391:1396	xylose	1391:1396	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	47	theme	activity-associated	1343:1361	arg1	galactose					1403:1411	galactose	1403:1411	galactose	1403:1411	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	47	theme	activity-associated	1343:1361	arg1	rhamnose					1381:1388	rhamnose	1381:1388	rhamnose	1381:1388	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	47	theme	activity-associated	1343:1361	arg1	mannose					1372:1378	mannose	1372:1378	mannose	1372:1378	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	3	48	theme	analysis	421:428	arg1	method					430:435	a multiple fingerprint analysis method	398:435	a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC)	398:580	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	4	49	theme	MLR	709:711	arg1	analysis					714:721	multiple linear regression (MLR) analysis	681:721	multiple linear regression (MLR) analysis	681:721	The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship.					
31707031	8	50	theme	quality	1565:1571	arg1	control					1573:1579	the quality control	1561:1579	the quality control of F. velutipes and its related products	1561:1620	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	0	51	theme	quality	63:69	arg1	assessment					71:80	quality assessment	63:80	quality assessment of polysaccharides from Flammulina velutipes	63:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	8	52	theme	F.	1584:1585	arg1	velutipes					1587:1595	F. velutipes	1584:1595	F. velutipes	1584:1595	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	8	53	theme	relationship	1505:1516	arg1	method					1518:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	2	54	theme	different	364:372	arg1	sources					374:380	different sources	364:380	different sources	364:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	8	55	theme	integrated	1537:1546	arg1	strategy					1548:1555	an integrated strategy	1534:1555	an integrated strategy for the quality control of F. velutipes and its related products	1534:1620	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	8	56	theme	HPLC	1479:1482	arg1	method					1518:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method	1465:1523	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	7	57	dep	markers	1363:1369	arg1	markers					1363:1369	activity-associated markers	1343:1369	activity-associated markers (mannose, rhamnose, xylose, and galactose)	1343:1412	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	57	dep	markers	1363:1369	arg1	xylose					1391:1396	xylose	1391:1396	xylose	1391:1396	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	57	dep	markers	1363:1369	arg1	galactose					1403:1411	galactose	1403:1411	galactose	1403:1411	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	57	dep	markers	1363:1369	arg1	rhamnose					1381:1388	rhamnose	1381:1388	rhamnose	1381:1388	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	57	dep	markers	1363:1369	arg1	mannose					1372:1378	mannose	1372:1378	mannose	1372:1378	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	6	58	theme	white	1089:1093	arg1	velutipes					1109:1117	white and yellow F. velutipes	1089:1117	white and yellow F. velutipes polysaccharides in HPLC fingerprints	1089:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	7	59	theme	growth	1311:1316	arg1	activity					1329:1336	the HepG2 growth inhibitory activity	1301:1336	the HepG2 growth inhibitory activity	1301:1336	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	60	theme	different	1446:1454	arg1	sources					1456:1462	different sources	1446:1462	different sources	1446:1462	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	5	61	theme	authentic	980:988	arg1	polysaccharides					990:1004	the authentic polysaccharides	976:1004	the authentic polysaccharides	976:1004	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	0	62	theme	polysaccharides	85:99	arg1	assessment					71:80	quality assessment	63:80	quality assessment of polysaccharides from Flammulina velutipes	63:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	0	63	from	velutipes	117:125	arg1	polysaccharides					85:99	polysaccharides	85:99	polysaccharides from Flammulina velutipes	85:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	0	63	from	velutipes	117:125	arg1	assessment					71:80	quality assessment	63:80	quality assessment of polysaccharides from Flammulina velutipes	63:125	Multiple fingerprint and fingerprint-activity relationship for quality assessment of polysaccharides from Flammulina velutipes.					
31707031	2	64	theme	assessment	276:285	arg1	method					287:292	no accurate and comprehensive assessment method	246:292	no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources	246:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	5	65	theme	different	880:888	arg1	velutipes					893:901	different F. velutipes	880:901	different F. velutipes	880:901	The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly.					
31707031	3	66	theme	high-performance	536:551	arg1	HPLC					576:579	HPLC	576:579	HPLC	576:579	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	66	theme	high-performance	536:551	arg1	chromatography					560:573	high-performance liquid chromatography	536:573	high-performance liquid chromatography (HPLC)	536:580	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	6	67	theme	HPLC	1138:1141	arg1	fingerprints					1143:1154	HPLC fingerprints	1138:1154	HPLC fingerprints	1138:1154	The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints.					
31707031	8	68	theme	related	1605:1611	arg1	products					1613:1620	its related products	1601:1620	its related products	1601:1620	The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.					
31707031	2	69	theme	comprehensive	262:274	arg1	method					287:292	no accurate and comprehensive assessment method	246:292	no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources	246:380	However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources.					
31707031	1	70	from	compounds	193:201	arg1	velutipes					217:225	Flammulina velutipes	206:225	Flammulina velutipes	206:225	Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes.					
31707031	3	71	theme	liquid	553:558	arg1	HPLC					576:579	HPLC	576:579	HPLC	576:579	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	3	71	theme	liquid	553:558	arg1	chromatography					560:573	high-performance liquid chromatography	536:573	high-performance liquid chromatography (HPLC)	536:580	In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established.					
31707031	7	72	theme	important	1283:1291	arg1	role					1293:1296	an important role	1280:1296	an important role	1280:1296	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31707031	7	73	theme	HepG2	1305:1309	arg1	activity					1329:1336	the HepG2 growth inhibitory activity	1301:1336	the HepG2 growth inhibitory activity	1301:1336	Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources.					
31642268	7	0	theme	spiked	1105:1110	arg1	samples					1131:1137	spiked Lycium barbarum L. samples	1105:1137	spiked Lycium barbarum L. samples	1105:1137	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	1	1	theme	fructose	269:276	arg1	monosaccharides					299:313	fructose and various aldehyde monosaccharides	269:313	monosaccharides	299:313	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	2	theme	various	282:288	arg1	monosaccharides					299:313	fructose and various aldehyde monosaccharides	269:313	monosaccharides	299:313	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	10	3	theme	planted	1681:1687	arg1	environmention					1689:1702	the planted environmention	1677:1702	the planted environmention	1677:1702	The concentration distribution of various monosaccharides differed notably depending on the planted environmention.					
31642268	6	4	from	mg/L	1045:1048	arg1	ranges					1024:1029	the ranges	1020:1029	the ranges	1020:1029	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	8	5	from	analysis	1341:1348	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides of Lycium barbarum L. from four planting areas	1387:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	5	6	theme	concentration	874:886	arg1	ranges					888:893	the corresponding mass concentration ranges	851:893	the corresponding mass concentration ranges	851:893	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	2	7	theme	gradient	563:570	arg1	elution					572:578	gradient elution	563:578	gradient elution	563:578	The separation was performed on a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution.					
31642268	8	8	theme	monosaccharide	1357:1370	arg1	composition					1372:1382	the monosaccharide composition	1353:1382	the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas	1353:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	0	9	theme	liquid	148:153	arg1	chromatography					155:168	high-performance liquid chromatography	131:168	high-performance liquid chromatography	131:168	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	1	10	theme	mass	451:454	arg1	HPLC-MS					470:476	HPLC-MS	470:476	HPLC-MS	470:476	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	10	theme	mass	451:454	arg1	spectrometry					456:467	mass spectrometry	451:467	mass spectrometry (HPLC-MS)	451:477	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	3	11	theme	mass	636:639	arg1	spectrometry					641:652	electrospray ionization mass spectrometry	612:652	electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode	612:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	3	12	theme	electrospray	612:623	arg1	spectrometry					641:652	electrospray ionization mass spectrometry	612:652	electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode	612:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	4	13	theme	fructose	752:759	arg1	mechanism					727:735	the derivatization mechanism	708:735	the derivatization mechanism of PMP-labeled fructose	708:759	In this study, the derivatization mechanism of PMP-labeled fructose was proposed under mild NH3·H2O conditions.					
31642268	6	14	dep	0.15	1062:1065	arg1	to					1059:1060	to	1059:1060	to	1059:1060	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	0	15	theme	mass	183:186	arg1	spectrometry					188:199	mass spectrometry	183:199	mass spectrometry	183:199	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	3	16	theme	ion	666:668	arg1	mode					687:690	selected ion monitoring (SIM) mode	657:690	selected ion monitoring (SIM) mode	657:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	5	17	theme	corresponding	855:867	arg1	ranges					888:893	the corresponding mass concentration ranges	851:893	the corresponding mass concentration ranges	851:893	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	2	18	with	column	527:532	arg1	elution					572:578	gradient elution	563:578	gradient elution	563:578	The separation was performed on a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution.					
31642268	4	19	theme	derivatization	712:725	arg1	mechanism					727:735	the derivatization mechanism	708:735	the derivatization mechanism of PMP-labeled fructose	708:759	In this study, the derivatization mechanism of PMP-labeled fructose was proposed under mild NH3·H2O conditions.					
31642268	7	20	theme	standard	1175:1182	arg1	RSDs					1196:1199	RSDs	1196:1199	RSDs	1196:1199	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	7	20	theme	standard	1175:1182	arg1	deviations					1184:1193	relative standard deviations	1166:1193	relative standard deviations (RSDs) of less than 10.2%	1166:1219	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	8	21	theme	planting	1435:1442	arg1	areas					1444:1448	four planting areas	1430:1448	four planting areas	1430:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	7	22	dep	%	1148:1148	arg1	to					1150:1151	to	1150:1151	to	1150:1151	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	11	23	theme	proposed	1709:1716	arg1	method					1718:1723	The proposed method	1705:1723	The proposed method	1705:1723	The proposed method is expected to be of great significance in standardizing the quality control of polysaccharides.					
31642268	9	24	theme	isolated	1475:1482	arg1	polysaccharides					1484:1498	the isolated polysaccharides	1471:1498	the isolated polysaccharides	1471:1498	Results showed that the isolated polysaccharides comprise mannose, fructose, rhamnose, galactose, glucose, xylose, arabinose, and ribose.					
31642268	6	25	theme	quantification	990:1003	arg1	mg/L					1045:1048	0.003 to 0.05 mg/L	1031:1048	0.003 to 0.05 mg/L	1031:1048	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	6	25	theme	quantification	990:1003	arg1	limits					980:985	limits	980:985	limits of quantification (LOQs)	980:1010	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	6	25	theme	quantification	990:1003	arg1	limits					949:954	The limits	945:954	The limits of detection (LODs)	945:974	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	7	26	dep	Lycium	1112:1117	arg1	barbarum					1119:1126	barbarum	1119:1126	barbarum	1119:1126	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	8	27	theme	repeatability	1278:1290	arg1	virtue					1225:1230	virtue	1225:1230	virtue of its simplicity, high sensitivity, and good repeatability	1225:1290	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	8	28	from	composition	1372:1382	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides of Lycium barbarum L. from four planting areas	1387:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	0	29	theme	mild	111:114	arg1	conditions					116:125	mild conditions	111:125	mild conditions	111:125	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	1	30	theme	liquid	416:421	arg1	chromatography					423:436	high-performance liquid chromatography	399:436	high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)	399:477	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	5	31	theme	suggested	809:817	arg1	method					819:824	The suggested method	805:824	The suggested method	805:824	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	1	32	theme	aldehyde	290:297	arg1	monosaccharides					299:313	fructose and various aldehyde monosaccharides	269:313	monosaccharides	299:313	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	4	33	theme	mild	780:783	arg1	conditions					793:802	mild NH3·H2O conditions	780:802	mild NH3·H2O conditions	780:802	In this study, the derivatization mechanism of PMP-labeled fructose was proposed under mild NH3·H2O conditions.					
31642268	0	34	theme	monosaccharide	13:26	arg1	composition					28:38	monosaccharide composition	13:38	monosaccharide composition in polysaccharides from Lycium barbarum L.	13:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	8	35	dep	Lycium	1406:1411	arg1	L.					1422:1423	Lycium barbarum L.	1406:1423	Lycium barbarum L. from four planting areas	1406:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	7	36	from	recoveries	1091:1100	arg1	samples					1131:1137	spiked Lycium barbarum L. samples	1105:1137	spiked Lycium barbarum L. samples	1105:1137	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	5	37	theme	good	833:836	arg1	linearity					838:846	good linearity	833:846	good linearity	833:846	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	1	38	dep	separation	220:229	arg1	the					216:218	the	216:218	the	216:218	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	11	39	theme	quality	1786:1792	arg1	control					1794:1800	the quality control	1782:1800	the quality control of polysaccharides	1782:1819	The proposed method is expected to be of great significance in standardizing the quality control of polysaccharides.					
31642268	6	40	from	mg/L	1067:1070	arg1	ranges					1024:1029	the ranges	1020:1029	the ranges	1020:1029	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	7	41	theme	relative	1166:1173	arg1	RSDs					1196:1199	RSDs	1196:1199	RSDs	1196:1199	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	7	41	theme	relative	1166:1173	arg1	deviations					1184:1193	relative standard deviations	1166:1193	relative standard deviations (RSDs) of less than 10.2%	1166:1219	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	1	42	with	labeling	344:351	arg1	PMP					390:392	PMP	390:392	PMP	390:392	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	42	with	labeling	344:351	arg1	1-phenyl-3-methyl-5-pyrazolone					358:387	1-phenyl-3-methyl-5-pyrazolone	358:387	1-phenyl-3-methyl-5-pyrazolone (PMP)	358:393	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	42	with	labeling	344:351	arg1	chromatography					423:436	high-performance liquid chromatography	399:436	high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)	399:477	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	8	43	theme	sensitivity	1256:1266	arg1	virtue					1225:1230	virtue	1225:1230	virtue of its simplicity, high sensitivity, and good repeatability	1225:1290	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	5	44	theme	correlation	905:915	arg1	r2					931:932	r2	931:932	r2	931:932	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	5	44	theme	correlation	905:915	arg1	coefficients					917:928	the correlation coefficients	901:928	the correlation coefficients (r2) > 0.9947	901:942	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	11	45	theme	great	1746:1750	arg1	significance					1752:1763	great significance	1746:1763	great significance	1746:1763	The proposed method is expected to be of great significance in standardizing the quality control of polysaccharides.					
31642268	0	46	theme	high-performance	131:146	arg1	chromatography					155:168	high-performance liquid chromatography	131:168	high-performance liquid chromatography	131:168	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	5	47	theme	mass	869:872	arg1	ranges					888:893	the corresponding mass concentration ranges	851:893	the corresponding mass concentration ranges	851:893	The suggested method showed good linearity in the corresponding mass concentration ranges, with the correlation coefficients (r2) > 0.9947.					
31642268	8	48	theme	composition	1372:1382	arg1	analysis					1341:1348	the analysis	1337:1348	the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas	1337:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	11	49	theme	polysaccharides	1805:1819	arg1	control					1794:1800	the quality control	1782:1800	the quality control of polysaccharides	1782:1819	The proposed method is expected to be of great significance in standardizing the quality control of polysaccharides.					
31642268	3	50	theme	ionization	625:634	arg1	spectrometry					641:652	electrospray ionization mass spectrometry	612:652	electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode	612:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	7	51	with	%	1158:1158	arg1	RSDs					1196:1199	RSDs	1196:1199	RSDs	1196:1199	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	7	51	with	%	1158:1158	arg1	deviations					1184:1193	relative standard deviations	1166:1193	relative standard deviations (RSDs) of less than 10.2%	1166:1219	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	0	52	from	[Analysis	0:8	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Lycium barbarum L.	43:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	6	53	theme	detection	959:967	arg1	mg/L					1045:1048	0.003 to 0.05 mg/L	1031:1048	0.003 to 0.05 mg/L	1031:1048	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	6	53	theme	detection	959:967	arg1	limits					980:985	limits	980:985	limits of quantification (LOQs)	980:1010	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	6	53	theme	detection	959:967	arg1	limits					949:954	The limits	945:954	The limits of detection (LODs)	945:974	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	8	54	theme	Lycium	1406:1411	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides of Lycium barbarum L. from four planting areas	1387:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	3	55	theme	selected	657:664	arg1	mode					687:690	selected ion monitoring (SIM) mode	657:690	selected ion monitoring (SIM) mode	657:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	4	56	theme	PMP-labeled	740:750	arg1	fructose					752:759	PMP-labeled fructose	740:759	PMP-labeled fructose	740:759	In this study, the derivatization mechanism of PMP-labeled fructose was proposed under mild NH3·H2O conditions.					
31642268	10	57	theme	various	1623:1629	arg1	monosaccharides					1631:1645	various monosaccharides	1623:1645	various monosaccharides	1623:1645	The concentration distribution of various monosaccharides differed notably depending on the planted environmention.					
31642268	8	58	from	polysaccharides	1387:1401	arg1	analysis					1341:1348	the analysis	1337:1348	the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas	1337:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	8	58	from	polysaccharides	1387:1401	arg1	areas					1444:1448	four planting areas	1430:1448	four planting areas	1430:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	0	59	from	composition	28:38	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Lycium barbarum L.	43:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	0	59	from	composition	28:38	arg1	Lycium					64:69	Lycium	64:69	Lycium	64:69	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	8	60	theme	simplicity	1239:1248	arg1	virtue					1225:1230	virtue	1225:1230	virtue of its simplicity, high sensitivity, and good repeatability	1225:1290	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	0	61	theme	precolumn	86:94	arg1	labeling					96:103	precolumn labeling	86:103	precolumn labeling	86:103	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	8	62	theme	high	1251:1254	arg1	sensitivity					1256:1266	high sensitivity	1251:1266	high sensitivity	1251:1266	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	2	63	dep	column	527:532	arg1	mm					546:547	100 mm×4.6 mm	535:547	100 mm×4.6 mm	535:547	The separation was performed on a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution.					
31642268	2	63	dep	column	527:532	arg1	μm					554:555	3.5 μm	550:555	3.5 μm	550:555	The separation was performed on a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution.					
31642268	0	64	from	Lycium	64:69	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Lycium barbarum L.	43:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	0	64	from	Lycium	64:69	arg1	composition					28:38	monosaccharide composition	13:38	monosaccharide composition in polysaccharides from Lycium barbarum L.	13:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	4	65	theme	NH3·H2O	785:791	arg1	conditions					793:802	mild NH3·H2O conditions	780:802	mild NH3·H2O conditions	780:802	In this study, the derivatization mechanism of PMP-labeled fructose was proposed under mild NH3·H2O conditions.					
31642268	3	66	from	spectrometry	641:652	arg1	mode					687:690	selected ion monitoring (SIM) mode	657:690	selected ion monitoring (SIM) mode	657:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	7	67	theme	116.2	1153:1157	arg1	%					1148:1148	%	1148:1148	%	1148:1148	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	1	68	theme	high-performance	399:414	arg1	chromatography					423:436	high-performance liquid chromatography	399:436	high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)	399:477	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	0	69	from	polysaccharides	43:57	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography	0:168	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	8	70	theme	good	1273:1276	arg1	repeatability					1278:1290	good repeatability	1273:1290	good repeatability	1273:1290	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	8	71	from	areas	1444:1448	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides of Lycium barbarum L. from four planting areas	1387:1448	By virtue of its simplicity, high sensitivity, and good repeatability, the method could be successfully applied to the analysis of the monosaccharide composition in polysaccharides of Lycium barbarum L. from four planting areas.					
31642268	0	72	theme	composition	28:38	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography	0:168	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	1	73	theme	monosaccharides	299:313	arg1	identification					232:245	identification	232:245	identification	232:245	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	73	theme	monosaccharides	299:313	arg1	determination					252:264	determination	252:264	determination	252:264	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	1	73	theme	monosaccharides	299:313	arg1	separation					220:229	separation	220:229	separation	220:229	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	3	74	theme	SIM	682:684	arg1	mode					687:690	selected ion monitoring (SIM) mode	657:690	selected ion monitoring (SIM) mode	657:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	10	75	theme	concentration	1593:1605	arg1	distribution					1607:1618	The concentration distribution	1589:1618	The concentration distribution of various monosaccharides	1589:1645	The concentration distribution of various monosaccharides differed notably depending on the planted environmention.					
31642268	7	76	theme	L.	1128:1129	arg1	samples					1131:1137	spiked Lycium barbarum L. samples	1105:1137	spiked Lycium barbarum L. samples	1105:1137	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	0	77	dep	Lycium	64:69	arg1	L.					80:81	Lycium barbarum L.	64:81	Lycium barbarum L.	64:81	[Analysis of monosaccharide composition in polysaccharides from Lycium barbarum L. by precolumn labeling under mild conditions and high-performance liquid chromatography coupled with mass spectrometry].					
31642268	2	78	theme	Kromasil-C18	514:525	arg1	column					527:532	a Kromasil-C18 column	512:532	a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution	512:578	The separation was performed on a Kromasil-C18 column (100 mm×4.6 mm, 3.5 μm) with gradient elution.					
31642268	3	79	theme	monitoring	670:679	arg1	mode					687:690	selected ion monitoring (SIM) mode	657:690	selected ion monitoring (SIM) mode	657:690	The detection was performed by electrospray ionization mass spectrometry in selected ion monitoring (SIM) mode.					
31642268	7	80	theme	Lycium	1112:1117	arg1	samples					1131:1137	spiked Lycium barbarum L. samples	1105:1137	spiked Lycium barbarum L. samples	1105:1137	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	1	81	theme	precolumn	334:342	arg1	labeling					344:351	precolumn labeling	334:351	precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)	334:477	A method for the separation, identification, and determination of fructose and various aldehyde monosaccharides was established by precolumn labeling with 1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).					
31642268	7	82	theme	%	1219:1219	arg1	RSDs					1196:1199	RSDs	1196:1199	RSDs	1196:1199	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	7	82	theme	%	1219:1219	arg1	deviations					1184:1193	relative standard deviations	1166:1193	relative standard deviations (RSDs) of less than 10.2%	1166:1219	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
31642268	10	83	theme	monosaccharides	1631:1645	arg1	distribution					1607:1618	The concentration distribution	1589:1618	The concentration distribution of various monosaccharides	1589:1645	The concentration distribution of various monosaccharides differed notably depending on the planted environmention.					
31642268	6	84	dep	0.05	1040:1043	arg1	to					1037:1038	to	1037:1038	to	1037:1038	The limits of detection (LODs) and limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 0.15 mg/L, respectively.					
31642268	7	85	theme	65.1	1144:1147	arg1	%					1148:1148	%	1148:1148	%	1148:1148	The recoveries in spiked Lycium barbarum L. samples were 65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%.					
30959857	5	0	theme	conjugates	816:825	arg1	presence					710:717	the presence	706:717	the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs	706:840	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	7	1	theme	SW480	1149:1153	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	2	theme	rugosa	519:524	arg1	Thunb					526:530	Rosa rugosa Thunb	514:530	Rosa rugosa Thunb	514:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	7	3	dep	enzymes	1001:1007	arg1	COX-2					1017:1021	COX-2	1017:1021	COX-2	1017:1021	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	3	dep	enzymes	1001:1007	arg1	COX-1					1010:1014	COX-1	1010:1014	COX-1	1010:1014	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	3	dep	enzymes	1001:1007	arg1	hyaluronidase					1024:1036	hyaluronidase	1024:1036	hyaluronidase	1024:1036	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	4	4	theme	cellular	597:604	arg1	processes					606:614	various cellular processes	589:614	various cellular processes involved in the development of cancer and other civilization diseases	589:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	7	5	theme	colon	1155:1159	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	6	theme	Thunb	526:530	arg1	petals					477:482	petals	477:482	petals	477:482	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	6	theme	Thunb	526:530	arg1	hips					493:496	hips	493:496	hips	493:496	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	6	theme	Thunb	526:530	arg1	achenes					503:509	achenes	503:509	achenes	503:509	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	7	7	theme	Different	915:923	arg1	extracts					945:952	Different polysaccharide-rich extracts	915:952	Different polysaccharide-rich extracts	915:952	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	4	8	theme	various	589:595	arg1	processes					606:614	various cellular processes	589:614	various cellular processes involved in the development of cancer and other civilization diseases	589:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	3	9	dep	extracts	433:440	arg1	polysaccharides					449:463	crude polysaccharides	443:463	crude polysaccharides	443:463	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	9	dep	extracts	433:440	arg1	CPLs					466:469	CPLs	466:469	CPLs	466:469	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	8	10	used	used	1280:1283	arg2	polysaccharides					1222:1236	rose crude polysaccharides	1211:1236	rose crude polysaccharides	1211:1236	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	10	used	used	1280:1283	arg2	agents					1312:1317	natural chemopreventive agents	1288:1317	natural chemopreventive agents	1288:1317	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	7	11	theme	polysaccharide-rich	925:943	arg1	extracts					945:952	Different polysaccharide-rich extracts	915:952	Different polysaccharide-rich extracts	915:952	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	12	from	activity	316:323	arg1	organs					352:357	rose organs	347:357	rose organs	347:357	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	8	13	theme	chemopreventive	1296:1310	arg1	polysaccharides					1222:1236	rose crude polysaccharides	1211:1236	rose crude polysaccharides	1211:1236	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	13	theme	chemopreventive	1296:1310	arg1	agents					1312:1317	natural chemopreventive agents	1288:1317	natural chemopreventive agents	1288:1317	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	7	14	from	activity	1118:1125	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	14	from	activity	1118:1125	arg1	lung					1140:1143	the A549 lung and SW480 colon cancer cell lines	1131:1177	lung	1140:1143	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	15	theme	unaddressed	383:393	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	15	theme	unaddressed	383:393	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	15	theme	unaddressed	383:393	arg1	activity					316:323	biological activity	305:323	biological activity	305:323	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	15	theme	unaddressed	383:393	arg1	content					293:299	content	293:299	content	293:299	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	7	16	from	effect	1061:1066	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	16	from	effect	1061:1066	arg1	lung					1140:1143	the A549 lung and SW480 colon cancer cell lines	1131:1177	lung	1140:1143	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	17	theme	cancer	1161:1166	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	0	18	from	Thunb.-Composition	47:64	arg1	Fractions					20:28	Polysaccharide-Rich Fractions	0:28	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.	0:95	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	5	19	theme	rose	830:833	arg1	organs					835:840	rose organs	830:840	rose organs	830:840	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	6	20	theme	Rose	843:846	arg1	source					888:893	the most abundant source	870:893	the most abundant source of polysaccharides	870:912	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	6	20	theme	Rose	843:846	arg1	hips					848:851	Rose hips	843:851	Rose hips	843:851	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	1	21	theme	huge	101:104	arg1	potential					124:132	The huge health-beneficial potential	97:132	The huge health-beneficial potential of polysaccharides	97:151	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	4	22	theme	diseases	677:684	arg1	development					632:642	the development	628:642	the development of cancer and other civilization diseases	628:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	3	23	from	achenes	503:509	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	23	from	achenes	503:509	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	1	24	theme	health-beneficial	106:122	arg1	potential					124:132	The huge health-beneficial potential	97:132	The huge health-beneficial potential of polysaccharides	97:151	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	7	25	from	enzymes	1001:1007	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	25	from	enzymes	1001:1007	arg1	lung					1140:1143	the A549 lung and SW480 colon cancer cell lines	1131:1177	lung	1140:1143	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	1	26	theme	compounds	219:227	arg1	sources					185:191	novel sources	179:191	novel sources	179:191	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	1	26	theme	compounds	219:227	arg1	applications					197:208	applications	197:208	applications	197:208	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	0	27	theme	Polysaccharide-Rich	0:18	arg1	Fractions					20:28	Polysaccharide-Rich Fractions	0:28	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.	0:95	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	7	28	theme	pro-inflammatory	984:999	arg1	enzymes					1001:1007	pro-inflammatory enzymes	984:1007	pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase)	984:1037	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	7	29	dep	in	1183:1184	arg1	vitro					1186:1190	vitro	1186:1190	vitro	1186:1190	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	0	30	theme	Rosa	35:38	arg1	Thunb.-Composition					47:64	Rosa rugosa Thunb.-Composition	35:64	Rosa rugosa Thunb.-Composition	35:64	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	4	31	from	influence	576:584	arg1	processes					606:614	various cellular processes	589:614	various cellular processes involved in the development of cancer and other civilization diseases	589:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	5	32	theme	water-soluble	722:734	arg1	polysaccharides					751:765	water-soluble and -insoluble polysaccharides	722:765	water-soluble and -insoluble polysaccharides (including β-glucans)	722:787	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	3	33	theme	polysaccharide-rich	413:431	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	33	theme	polysaccharide-rich	413:431	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	7	34	theme	scavenging	1050:1059	arg1	effect					1061:1066	a radical scavenging effect	1040:1066	a radical scavenging effect (against DPPH• and ABTS•+)	1040:1093	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	1	35	theme	polysaccharides	137:151	arg1	potential					124:132	The huge health-beneficial potential	97:132	The huge health-beneficial potential of polysaccharides	97:151	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	4	36	theme	cancer	647:652	arg1	development					632:642	the development	628:642	the development of cancer and other civilization diseases	628:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	4	37	from	composition	556:566	arg1	processes					606:614	various cellular processes	589:614	various cellular processes involved in the development of cancer and other civilization diseases	589:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	3	38	from	content	293:299	arg1	organs					352:357	rose organs	347:357	rose organs	347:357	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	5	39	theme	-insoluble	740:749	arg1	polysaccharides					751:765	water-soluble and -insoluble polysaccharides	722:765	water-soluble and -insoluble polysaccharides (including β-glucans)	722:787	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	6	40	theme	polysaccharides	898:912	arg1	hips					848:851	Rose hips	843:851	Rose hips	843:851	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	6	40	theme	polysaccharides	898:912	arg1	source					888:893	the most abundant source	870:893	the most abundant source of polysaccharides	870:912	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	5	41	theme	polysaccharides	751:765	arg1	presence					710:717	the presence	706:717	the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs	706:840	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	5	42	attach	presence	710:717	arg2	polysaccharides					751:765	water-soluble and -insoluble polysaccharides	722:765	water-soluble and -insoluble polysaccharides (including β-glucans)	722:787	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	5	42	attach	presence	710:717	arg2	conjugates					816:825	protein-polysaccharide conjugates	793:825	protein-polysaccharide conjugates	793:825	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	5	42	attach	presence	710:717	arg1	organs					835:840	rose organs	830:840	rose organs	830:840	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	4	43	theme	civilization	664:675	arg1	diseases					677:684	other civilization diseases	658:684	other civilization diseases	658:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	0	44	theme	rugosa	40:45	arg1	Thunb.-Composition					47:64	Rosa rugosa Thunb.-Composition	35:64	Rosa rugosa Thunb.-Composition	35:64	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	3	45	theme	biological	305:314	arg1	activity					316:323	biological activity	305:323	biological activity	305:323	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	45	theme	biological	305:314	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	45	theme	biological	305:314	arg1	content					293:299	content	293:299	content	293:299	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	8	46	theme	natural	1288:1294	arg1	polysaccharides					1222:1236	rose crude polysaccharides	1211:1236	rose crude polysaccharides	1211:1236	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	46	theme	natural	1288:1294	arg1	agents					1312:1317	natural chemopreventive agents	1288:1317	natural chemopreventive agents	1288:1317	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	47	theme	rose	1211:1214	arg1	polysaccharides					1222:1236	rose crude polysaccharides	1211:1236	rose crude polysaccharides	1211:1236	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	47	theme	rose	1211:1214	arg1	agents					1312:1317	natural chemopreventive agents	1288:1317	natural chemopreventive agents	1288:1317	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	3	48	from	leaves	485:490	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	48	from	leaves	485:490	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	4	49	theme	other	658:662	arg1	diseases					677:684	other civilization diseases	658:684	other civilization diseases	658:684	were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases.					
30959857	3	50	theme	crude	443:447	arg1	polysaccharides					449:463	crude polysaccharides	443:463	crude polysaccharides	443:463	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	50	theme	crude	443:447	arg1	CPLs					466:469	CPLs	466:469	CPLs	466:469	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	51	theme	rose	347:350	arg1	organs					352:357	rose organs	347:357	rose organs	347:357	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	0	52	from	Potential	86:94	arg1	Fractions					20:28	Polysaccharide-Rich Fractions	0:28	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.	0:95	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	2	53	theme	polysaccharides	260:274	arg1	rose					283:286	the rose	279:286	the rose	279:286	One poorly explored source of polysaccharides is the rose.					
30959857	2	53	theme	polysaccharides	260:274	arg1	source					250:255	One poorly explored source	230:255	One poorly explored source of polysaccharides	230:274	One poorly explored source of polysaccharides is the rose.					
30959857	3	54	dep	content	293:299	arg1	The					289:291	The	289:291	The	289:291	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	7	55	theme	antiproliferative	1100:1116	arg1	activity					1118:1125	antiproliferative activity	1100:1125	antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines)	1100:1178	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	6	56	theme	abundant	879:886	arg1	hips					848:851	Rose hips	843:851	Rose hips	843:851	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	6	56	theme	abundant	879:886	arg1	source					888:893	the most abundant source	870:893	the most abundant source of polysaccharides	870:912	Rose hips were found to be the most abundant source of polysaccharides.					
30959857	7	57	theme	A549	1135:1138	arg1	lung					1140:1143	the A549 lung and SW480 colon cancer cell lines	1131:1177	lung	1140:1143	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	58	from	hips	493:496	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	58	from	hips	493:496	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	59	theme	Rosa	514:517	arg1	Thunb					526:530	Rosa rugosa Thunb	514:530	Rosa rugosa Thunb	514:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	60	theme	polysaccharides	328:342	arg1	activity					316:323	biological activity	305:323	biological activity	305:323	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	60	theme	polysaccharides	328:342	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	60	theme	polysaccharides	328:342	arg1	content					293:299	content	293:299	content	293:299	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	2	61	theme	explored	241:248	arg1	rose					283:286	the rose	279:286	the rose	279:286	One poorly explored source of polysaccharides is the rose.					
30959857	2	61	theme	explored	241:248	arg1	source					250:255	One poorly explored source	230:255	One poorly explored source of polysaccharides	230:274	One poorly explored source of polysaccharides is the rose.					
30959857	7	62	theme	cell	1168:1171	arg1	lines					1173:1177	the A549 lung and SW480 colon cancer cell lines	1131:1177	lines	1173:1177	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	5	63	theme	protein-polysaccharide	793:814	arg1	conjugates					816:825	protein-polysaccharide conjugates	793:825	protein-polysaccharide conjugates	793:825	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	5	64	from	presence	710:717	arg1	organs					835:840	rose organs	830:840	rose organs	830:840	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30959857	8	65	theme	crude	1216:1220	arg1	polysaccharides					1222:1236	rose crude polysaccharides	1211:1236	rose crude polysaccharides	1211:1236	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	8	65	theme	crude	1216:1220	arg1	agents					1312:1317	natural chemopreventive agents	1288:1317	natural chemopreventive agents	1288:1317	Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.					
30959857	7	66	theme	radical	1042:1048	arg1	effect					1061:1066	a radical scavenging effect	1040:1066	a radical scavenging effect (against DPPH• and ABTS•+)	1040:1093	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	1	67	theme	novel	179:183	arg1	sources					185:191	novel sources	179:191	novel sources	179:191	The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds.					
30959857	0	68	theme	Chemopreventive	70:84	arg1	Potential					86:94	Chemopreventive Potential	70:94	Chemopreventive Potential	70:94	Polysaccharide-Rich Fractions from Rosa rugosa Thunb.-Composition and Chemopreventive Potential.					
30959857	7	69	theme	in	1183:1184	arg1	assays					1192:1197	in vitro assays	1183:1197	in vitro assays	1183:1197	Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays.					
30959857	3	70	from	petals	477:482	arg1	extracts					433:440	polysaccharide-rich extracts	413:440	polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb	413:530	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	3	70	from	petals	477:482	arg1	topic					395:399	an almost completely unaddressed topic	362:399	an almost completely unaddressed topic	362:399	The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb.					
30959857	5	71	dep	polysaccharides	751:765	arg1	including					768:776	including	768:776	including β-glucans	768:786	The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs.					
30682481	2	0	theme	structural	470:479	arg1	homogeneity					481:491	structural homogeneity	470:491	structural homogeneity	470:491	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	5	1	theme	degradation	1051:1061	arg1	rate					1063:1066	degradation rate	1051:1066	degradation rate	1051:1066	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	4	2	theme	washed	858:863	arg1	PECs					865:868	the washed PECs	854:868	the washed PECs	854:868	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	1	3	theme	durable	157:163	arg1	complexes					181:189	durable polyelectrolyte complexes	157:189	durable polyelectrolyte complexes (hydrogel PECs)	157:205	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	1	3	theme	durable	157:163	arg1	PECs					201:204	hydrogel PECs	192:204	hydrogel PECs	192:204	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	6	4	theme	CS/GG-based	1098:1108	arg1	hydrogels					1110:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	0	5	theme	assemblies	88:97	arg1	durability					27:36	durability	27:36	durability	27:36	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	0	5	theme	assemblies	88:97	arg1	homogeneity					53:63	structural homogeneity	42:63	structural homogeneity	42:63	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	3	6	theme	weight	641:646	arg1	ratios					648:653	CS:GG solutions (at 60 °C) with GG:CS weight ratios	603:653	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	4	7	theme	high	824:827	arg1	degree					829:834	a high degree	822:834	a high degree of association	822:849	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	1	8	theme	polyelectrolyte	165:179	arg1	complexes					181:189	durable polyelectrolyte complexes	157:189	durable polyelectrolyte complexes (hydrogel PECs)	157:205	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	1	8	theme	polyelectrolyte	165:179	arg1	PECs					201:204	hydrogel PECs	192:204	hydrogel PECs	192:204	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	2	9	theme	aqueous	521:527	arg1	instability					529:539	aqueous instability	521:539	aqueous instability	521:539	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	4	10	theme	CS-GG	738:742	arg1	chains					744:749	CS-GG chains	738:749	CS-GG chains	738:749	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	4	11	theme	association	839:849	arg1	degree					829:834	a high degree	822:834	a high degree of association	822:849	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	6	12	theme	chemical	1180:1187	arg1	processes					1189:1197	chemical processes	1180:1197	chemical processes	1180:1197	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	4	13	theme	spectroscopy	699:710	arg1	analysis					718:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	3	14	from	60 °C	623:627	arg1	solutions					609:617	GG solutions	606:617	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	1	15	theme	GG	334:335	arg1	hydrogels					350:358	GG and CS-based hydrogels	334:358	GG and CS-based hydrogels	334:358	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	4	16	theme	chains	916:921	arg1	self-assembling					889:903	self-assembling	889:903	self-assembling	889:903	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	5	17	theme	GG	931:932	arg1	ratio					941:945	GG weight ratio	931:945	GG weight ratio	931:945	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	2	18	theme	stable	427:432	arg1	hydrogels					455:463	stable and durable GG-based hydrogels	427:463	stable and durable GG-based hydrogels	427:463	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	1	19	theme	CS-based	341:348	arg1	hydrogels					350:358	GG and CS-based hydrogels	334:358	GG and CS-based hydrogels	334:358	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	3	20	with	solutions	609:617	arg1	GG					635:636	GG	635:636	GG	635:636	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	4	21	theme	polymer	908:914	arg1	chains					916:921	polymer chains	908:921	polymer chains	908:921	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	5	22	theme	porous	1027:1032	arg1	homogeneity					1034:1044	porous homogeneity	1027:1044	porous homogeneity	1027:1044	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	1	23	theme	hydrogel	192:199	arg1	complexes					181:189	durable polyelectrolyte complexes	157:189	durable polyelectrolyte complexes (hydrogel PECs)	157:205	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	1	23	theme	hydrogel	192:199	arg1	PECs					201:204	hydrogel PECs	192:204	hydrogel PECs	192:204	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	0	24	theme	Chitosan	0:7	arg1	content					9:15	Chitosan content	0:15	Chitosan content	0:15	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	6	25	theme	conventional	1145:1156	arg1	materials					1158:1166	conventional materials	1145:1166	conventional materials produced by chemical processes	1145:1197	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	4	26	theme	photoelectron	685:697	arg1	analysis					718:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	3	27	theme	CS	603:604	arg1	ratios					648:653	CS:GG solutions (at 60 °C) with GG:CS weight ratios	603:653	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	4	28	theme	characteristic	871:884	arg1	forces					803:808	electrostatic and intermolecular forces	770:808	electrostatic and intermolecular forces	770:808	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	4	29	theme	XPS	713:715	arg1	analysis					718:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	3	30	dep	CS	603:604	arg1	solutions					609:617	GG solutions	606:617	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	3	30	dep	CS	603:604	arg1	CS					638:639	CS	638:639	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	6	31	theme	thermosensitive	1082:1096	arg1	hydrogels					1110:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	2	32	theme	typical	542:548	arg1	precipitation					503:515	precipitation	503:515	precipitation	503:515	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	5	33	theme	high	1001:1004	arg1	porosity					1006:1013	polyelectrolyte complex (PEC) high porosity	971:1013	polyelectrolyte complex (PEC) high porosity	971:1013	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	6	34	theme	Physical	1069:1076	arg1	hydrogels					1110:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	0	35	theme	structural	42:51	arg1	homogeneity					53:63	structural homogeneity	42:63	structural homogeneity	42:63	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	1	36	theme	metallic	266:273	arg1	agents					301:306	metallic and covalent crosslinking agents	266:306	metallic and covalent crosslinking agents	266:306	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	4	37	theme	self-assembling	889:903	arg1	characteristic					871:884	characteristic	871:884	characteristic	871:884	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	2	38	theme	new	380:382	arg1	approach					384:391	This new approach	375:391	This new approach	375:391	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	4	39	theme	electrostatic	770:782	arg1	forces					803:808	electrostatic and intermolecular forces	770:808	electrostatic and intermolecular forces	770:808	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	1	40	theme	gellan	212:217	arg1	GG					224:225	GG	224:225	GG	224:225	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	1	40	theme	gellan	212:217	arg1	gum					219:221	gellan gum	212:221	gellan gum (GG)	212:226	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	2	41	theme	GG-based	446:453	arg1	hydrogels					455:463	stable and durable GG-based hydrogels	427:463	stable and durable GG-based hydrogels	427:463	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	2	42	theme	durable	438:444	arg1	hydrogels					455:463	stable and durable GG-based hydrogels	427:463	stable and durable GG-based hydrogels	427:463	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	1	43	theme	covalent	279:286	arg1	agents					301:306	metallic and covalent crosslinking agents	266:306	metallic and covalent crosslinking agents	266:306	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	4	44	theme	intermolecular	788:801	arg1	forces					803:808	electrostatic and intermolecular forces	770:808	electrostatic and intermolecular forces	770:808	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	1	45	theme	new	117:119	arg1	method					141:146	a new and straightforward method	115:146	a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively	115:372	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	1	46	theme	crosslinking	288:299	arg1	agents					301:306	metallic and covalent crosslinking agents	266:306	metallic and covalent crosslinking agents	266:306	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	5	47	theme	complex	987:993	arg1	porosity					1006:1013	polyelectrolyte complex (PEC) high porosity	971:1013	polyelectrolyte complex (PEC) high porosity	971:1013	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	5	48	theme	polyelectrolyte	971:985	arg1	porosity					1006:1013	polyelectrolyte complex (PEC) high porosity	971:1013	polyelectrolyte complex (PEC) high porosity	971:1013	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	2	49	theme	materials	563:571	arg1	typical					542:548	typical	542:548	typical	542:548	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	5	50	theme	weight	934:939	arg1	ratio					941:945	GG weight ratio	931:945	GG weight ratio	931:945	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	3	51	theme	GG	606:607	arg1	solutions					609:617	GG solutions	606:617	CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60	603:676	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
30682481	1	52	theme	straightforward	125:139	arg1	method					141:146	a new and straightforward method	115:146	a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively	115:372	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	2	53	theme	PEC-based	553:561	arg1	materials					563:571	PEC-based materials	553:571	PEC-based materials	553:571	This new approach overcomes challenges of obtaining stable and durable GG-based hydrogels with structural homogeneity, avoiding precipitation and aqueous instability, typical of PEC-based materials.					
30682481	5	54	theme	PEC	996:998	arg1	porosity					1006:1013	polyelectrolyte complex (PEC) high porosity	971:1013	polyelectrolyte complex (PEC) high porosity	971:1013	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	1	55	dep	gum	219:221	arg1	assemblies					246:255	assemblies	246:255	assemblies	246:255	Here we report a new and straightforward method to yield durable polyelectrolyte complexes (hydrogel PECs) from gellan gum (GG) and chitosan (CS) assemblies, without metallic and covalent crosslinking agents, commonly used to produce GG and CS-based hydrogels, respectively.					
30682481	5	56	dep	CS	928:929	arg1	tuned					954:958	tuned	954:958	can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate	947:1066	The CS:GG weight ratio can be tuned to improve polyelectrolyte complex (PEC) high porosity, stability, porous homogeneity, and degradation rate.					
30682481	4	57	theme	X-ray	679:683	arg1	analysis					718:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis	679:725	X-ray photoelectron spectroscopy (XPS) analysis shows that CS-GG chains are interacting by electrostatic and intermolecular forces, conferring a high degree of association to the washed PECs, characteristic of self-assembling of polymer chains.					
30682481	0	58	theme	gum	84:86	arg1	assemblies					88:97	chitosan-gellan gum assemblies	68:97	chitosan-gellan gum assemblies	68:97	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	0	59	gly	homogeneity	53:63	arg1	assemblies					88:97	chitosan-gellan gum assemblies	68:97	chitosan-gellan gum assemblies	68:97	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	6	60	contain	have	1124:1127	arg2	advantages					1129:1138	advantages	1129:1138	advantages	1129:1138	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	6	60	contain	have	1124:1127	arg1	hydrogels					1110:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels	1069:1118	Physical and thermosensitive CS/GG-based hydrogels can have advantages over conventional materials produced by chemical processes.					
30682481	0	61	theme	chitosan-gellan	68:82	arg1	assemblies					88:97	chitosan-gellan gum assemblies	68:97	chitosan-gellan gum assemblies	68:97	Chitosan content modulates durability and structural homogeneity of chitosan-gellan gum assemblies.					
30682481	3	62	dep	40:60	672:676	arg1	to					669:670	to	669:670	to	669:670	PECs are created by blending CS:GG solutions (at 60 °C) with GG:CS weight ratios between 80:20 to 40:60.					
31348962	0	0	theme	polysaccharides	98:112	arg1	production					39:48	production	39:48	production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris	39:147	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	2	1	theme	types	482:486	arg1	production					463:472	the simultaneous production	446:472	the simultaneous production of both types of cell-wall polysaccharides	446:515	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	6	2	theme	fewer	1185:1189	arg1	components					1191:1200	fewer components	1185:1200	fewer components	1185:1200	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	6	3	theme	reduced	1220:1226	arg1	content					1234:1240	considerably reduced salts content	1207:1240	considerably reduced salts content	1207:1240	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	5	4	theme	CGC	1155:1157	arg1	cultivation					1101:1111	cultivation	1101:1111	cultivation of K. pastoris	1101:1126	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	5	4	theme	CGC	1155:1157	arg1	production					1136:1145	the production	1132:1145	the production of both CGC and mannans	1132:1169	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	3	5	theme	previous	776:783	arg1	studies					785:791	previous studies	776:791	previous studies	776:791	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	0	6	theme	mannose-containing	79:96	arg1	polysaccharides					98:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	3	7	dep	K.	561:562	arg1	pastoris					564:571	K. pastoris	561:571	K. pastoris	561:571	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	4	8	contain	had	811:813	arg1	CGC					807:809	The produced CGC	794:809	The produced CGC	794:809	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	4	8	contain	had	811:813	arg2	ratio					839:843	a chitin:β-glucan molar ratio	815:843	a chitin:β-glucan molar ratio of 12:88	815:852	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	4	9	theme	reported	879:886	arg1	values					888:893	previously reported values	868:893	previously reported values for K. pastoris CGC (11:89-19:81)	868:927	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	3	10	theme	K	536:536	arg1	use					522:524	The use	518:524	The use of Medium K for the cultivation of K. pastoris	518:571	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	4	11	theme	mannose	972:978	arg1	units					980:984	mannose units	972:984	mannose units	972:984	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	5	12	theme	K.	1116:1117	arg1	pastoris					1119:1126	K. pastoris	1116:1126	K. pastoris	1116:1126	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	6	13	theme	significant	1263:1273	arg1	simplification					1275:1288	a significant simplification	1261:1288	a significant simplification of the bioprocess with no precipitation problems	1261:1337	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	3	14	dep	productivities	711:724	arg1	19.2 g/L day					736:747	19.2 g/L day	736:747	19.2 g/L day	736:747	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	3	14	dep	productivities	711:724	arg1	17.5					727:730	17.5	727:730	17.5	727:730	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	4	15	theme	chitin	817:822	arg1	ratio					839:843	a chitin:β-glucan molar ratio	815:843	a chitin:β-glucan molar ratio of 12:88	815:852	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	5	16	theme	pastoris	1119:1126	arg1	cultivation					1101:1111	cultivation	1101:1111	cultivation of K. pastoris	1101:1126	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	5	16	theme	pastoris	1119:1126	arg1	production					1136:1145	the production	1132:1145	the production of both CGC and mannans	1132:1169	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	0	17	theme	Komagataella	127:138	arg1	pastoris					140:147	the yeast Komagataella pastoris	117:147	the yeast Komagataella pastoris	117:147	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	4	18	with	units	980:984	arg1	content					1002:1008	a protein content	992:1008	a protein content of 10 wt%	992:1018	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	2	19	theme	cultivation	391:401	arg1	medium					403:408	a novel cultivation medium	383:408	a novel cultivation medium	383:408	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	2	19	theme	cultivation	391:401	arg1	K					418:418	Medium K	411:418	Medium K	411:418	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	0	20	theme	yeast	121:125	arg1	pastoris					140:147	the yeast Komagataella pastoris	117:147	the yeast Komagataella pastoris	117:147	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	3	21	theme	Medium	529:534	arg1	K					536:536	Medium K	529:536	Medium K	529:536	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	2	22	theme	novel	385:389	arg1	medium					403:408	a novel cultivation medium	383:408	a novel cultivation medium	383:408	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	2	22	theme	novel	385:389	arg1	K					418:418	Medium K	411:418	Medium K	411:418	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	6	23	theme	precipitation	1316:1328	arg1	problems					1330:1337	no precipitation problems	1313:1337	no precipitation problems	1313:1337	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	1	24	theme	mannose-containing	290:307	arg1	mannans					319:325	mannans	319:325	mannans	319:325	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	24	theme	mannose-containing	290:307	arg1	polymers					309:316	mannose-containing polymers	290:316	mannose-containing polymers (mannans)	290:326	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	4	25	theme	produced	798:805	arg1	CGC					807:809	The produced CGC	794:809	The produced CGC	794:809	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	3	26	theme	K.	561:562	arg1	cultivation					546:556	the cultivation	542:556	the cultivation of K. pastoris	542:571	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	1	27	theme	valuable	209:216	arg1	polysaccharides					218:232	valuable polysaccharides	209:232	valuable polysaccharides	209:232	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	0	28	theme	composition	23:33	arg1	Optimization					0:11	Optimization	0:11	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.	0:148	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	1	29	theme	polymers	309:316	arg1	pastoris					163:170	Komagataella pastoris	150:170	Komagataella pastoris	150:170	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	29	theme	polymers	309:316	arg1	source					199:204	a source	197:204	a source	197:204	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	30	theme	polysaccharides	218:232	arg1	pastoris					163:170	Komagataella pastoris	150:170	Komagataella pastoris	150:170	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	30	theme	polysaccharides	218:232	arg1	source					199:204	a source	197:204	a source	197:204	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	0	31	theme	medium	16:21	arg1	composition					23:33	medium composition	16:33	medium composition	16:33	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	3	32	theme	higher	683:688	arg1	products					690:697	significantly higher products	669:697	significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively)	669:762	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	4	33	theme	12:88	848:852	arg1	ratio					839:843	a chitin:β-glucan molar ratio	815:843	a chitin:β-glucan molar ratio of 12:88	815:852	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	4	34	theme	%	1018:1018	arg1	content					1002:1008	a protein content	992:1008	a protein content of 10 wt%	992:1018	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	2	35	theme	Medium	411:416	arg1	medium					403:408	a novel cultivation medium	383:408	a novel cultivation medium	383:408	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	2	35	theme	Medium	411:416	arg1	K					418:418	Medium K	411:418	Medium K	411:418	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	4	36	theme	10 wt	1013:1017	arg1	%					1018:1018	10 wt%	1013:1018	10 wt%	1013:1018	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	3	37	theme	volumetric	700:709	arg1	productivities					711:724	volumetric productivities	700:724	significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively)	669:762	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	6	38	theme	bioprocess	1297:1306	arg1	simplification					1275:1288	a significant simplification	1261:1288	a significant simplification of the bioprocess with no precipitation problems	1261:1337	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	6	39	theme	salts	1228:1232	arg1	content					1234:1240	considerably reduced salts content	1207:1240	considerably reduced salts content	1207:1240	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	3	40	theme	high	585:588	arg1	contents					590:597	high contents	585:597	high contents of CGC (19 wt%)	585:613	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	2	41	theme	polysaccharides	501:515	arg1	types					482:486	both types	477:486	both types of cell-wall polysaccharides	477:515	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	2	42	theme	simultaneous	450:461	arg1	production					463:472	the simultaneous production	446:472	the simultaneous production of both types of cell-wall polysaccharides	446:515	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	4	43	theme	molar	833:837	arg1	ratio					839:843	a chitin:β-glucan molar ratio	815:843	a chitin:β-glucan molar ratio of 12:88	815:852	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	0	44	theme	complex	67:73	arg1	polysaccharides					98:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	6	45	theme	no	1313:1314	arg1	problems					1330:1337	no precipitation problems	1313:1337	no precipitation problems	1313:1337	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	5	46	theme	developed	1057:1065	arg1	suitable					1088:1095	suitable	1088:1095	suitable	1088:1095	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	5	46	theme	developed	1057:1065	arg1	medium					1077:1082	the developed optimized medium	1053:1082	the developed optimized medium	1053:1082	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	4	47	theme	β-glucan	824:831	arg1	ratio					839:843	a chitin:β-glucan molar ratio	815:843	a chitin:β-glucan molar ratio of 12:88	815:852	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	3	48	dep	products	690:697	arg1	productivities					711:724	volumetric productivities	700:724	significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively)	669:762	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	5	49	theme	optimized	1067:1075	arg1	suitable					1088:1095	suitable	1088:1095	suitable	1088:1095	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	5	49	theme	optimized	1067:1075	arg1	medium					1077:1082	the developed optimized medium	1053:1082	the developed optimized medium	1053:1082	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	3	50	theme	CGC	602:604	arg1	mannans					619:625	mannans	619:625	mannans (21 wt%)	619:634	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	3	50	theme	CGC	602:604	arg1	contents					590:597	high contents	585:597	high contents of CGC (19 wt%)	585:613	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	3	50	theme	CGC	602:604	arg1	%					633:633	21 wt%	628:633	21 wt%	628:633	The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies.					
31348962	6	51	with	bioprocess	1297:1306	arg1	problems					1330:1337	no precipitation problems	1313:1337	no precipitation problems	1313:1337	It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.					
31348962	4	52	dep	K.	899:900	arg1	pastoris					902:909	K. pastoris CGC (11:89-19:81)	899:927	K. pastoris CGC (11:89-19:81)	899:927	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	4	52	dep	K.	899:900	arg1	CGC					911:913	CGC (11:89-19:81)	911:927	K. pastoris CGC (11:89-19:81)	899:927	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	4	53	theme	protein	994:1000	arg1	content					1002:1008	a protein content	992:1008	a protein content of 10 wt%	992:1018	The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%.					
31348962	1	54	theme	co-polymer	247:256	arg1	CGC					281:283	CGC	281:283	CGC	281:283	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	54	theme	co-polymer	247:256	arg1	complex					272:278	the co-polymer chitin-glucan complex	243:278	the co-polymer chitin-glucan complex (CGC)	243:284	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	2	55	theme	cell-wall	491:499	arg1	polysaccharides					501:515	cell-wall polysaccharides	491:515	cell-wall polysaccharides	491:515	In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides.					
31348962	5	56	theme	mannans	1163:1169	arg1	cultivation					1101:1111	cultivation	1101:1111	cultivation of K. pastoris	1101:1126	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	5	56	theme	mannans	1163:1169	arg1	production					1136:1145	the production	1132:1145	the production of both CGC and mannans	1132:1169	These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans.					
31348962	1	57	theme	chitin-glucan	258:270	arg1	CGC					281:283	CGC	281:283	CGC	281:283	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	57	theme	chitin-glucan	258:270	arg1	complex					272:278	the co-polymer chitin-glucan complex	243:278	the co-polymer chitin-glucan complex (CGC)	243:284	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	0	58	theme	chitin-glucan	53:65	arg1	polysaccharides					98:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	chitin-glucan complex and mannose-containing polysaccharides	53:112	Optimization of medium composition for production of chitin-glucan complex and mannose-containing polysaccharides by the yeast Komagataella pastoris.					
31348962	1	59	theme	complex	272:278	arg1	pastoris					163:170	Komagataella pastoris	150:170	Komagataella pastoris	150:170	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
31348962	1	59	theme	complex	272:278	arg1	source					199:204	a source	197:204	a source	197:204	Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall.					
30980844	0	0	theme	fibroblast	95:104	arg1	cells					106:110	L929 mouse fibroblast cells	84:110	L929 mouse fibroblast cells	84:110	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	5	1	theme	mouse	768:772	arg1	cells					785:789	L929 mouse fibroblast cells	763:789	L929 mouse fibroblast cells	763:789	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	7	2	theme	basal	1148:1152	arg1	activity					1154:1161	GLUT1's basal activity	1140:1161	GLUT1's basal activity	1140:1161	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	8	3	theme	cytoskeleton	1372:1383	arg1	disruption					1348:1357	disruption	1348:1357	disruption of the actin cytoskeleton or microtubules	1348:1399	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	10	4	from	activation	1807:1816	arg1	L929 cell					1821:1829	L929 cell	1821:1829	L929 cell	1821:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	6	5	theme	X-100	1030:1034	arg1	extraction					1036:1045	cold Triton X-100 extraction	1018:1045	cold Triton X-100 extraction	1018:1045	These structures are not altered by cholesterol removal with methyl-β-cyclodextrin and lack resistance to cold Triton X-100 extraction.					
30980844	0	6	theme	mouse	89:93	arg1	cells					106:110	L929 mouse fibroblast cells	84:110	L929 mouse fibroblast cells	84:110	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	2	7	theme	cells	331:335	arg1	variety					320:326	a wide variety	313:326	a wide variety of cells	313:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	2	7	theme	cells	331:335	arg1	cells					331:335	cells	331:335	cells	331:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	6	8	theme	Triton	1023:1028	arg1	extraction					1036:1045	cold Triton X-100 extraction	1018:1045	cold Triton X-100 extraction	1018:1045	These structures are not altered by cholesterol removal with methyl-β-cyclodextrin and lack resistance to cold Triton X-100 extraction.					
30980844	5	9	from	phenomenon	749:758	arg1	cells					785:789	L929 mouse fibroblast cells	763:789	L929 mouse fibroblast cells	763:789	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	10	10	theme	low	1755:1757	arg1	fractions					1776:1784	low density membrane fractions	1755:1784	low density membrane fractions in response to acute activation in L929 cell	1755:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	9	11	theme	membrane	1651:1658	arg1	fractions					1660:1668	membrane fractions	1651:1668	membrane fractions	1651:1668	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	8	12	theme	microtubules	1388:1399	arg1	disruption					1348:1357	disruption	1348:1357	disruption of the actin cytoskeleton or microtubules	1348:1399	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	2	13	from	uptake	303:308	arg1	variety					320:326	a wide variety	313:326	a wide variety of cells	313:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	2	13	from	uptake	303:308	arg1	cells					331:335	cells	331:335	cells	331:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	4	14	theme	Previous	596:603	arg1	findings					605:612	Previous findings	596:612	Previous findings	596:612	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	0	15	from	domains	73:79	arg1	cells					106:110	L929 mouse fibroblast cells	84:110	L929 mouse fibroblast cells	84:110	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	10	16	dep	fractions	1776:1784	arg1	out					1748:1750	out	1748:1750	out	1748:1750	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	9	17	theme	pharmacologic	1573:1585	arg1	inhibition					1587:1596	pharmacologic inhibition	1573:1596	pharmacologic inhibition of these processes	1573:1615	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	4	18	theme	uptake	712:717	arg1	activity					719:726	GLUT1 uptake activity	706:726	GLUT1 uptake activity	706:726	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	5	19	theme	low	833:835	arg1	microdomain					851:861	a low density lipid microdomain	831:861	a low density lipid microdomain distinct from traditionally-defined lipid rafts	831:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	4	20	theme	membrane	634:641	arg1	subdomains					643:652	plasma membrane subdomains	627:652	plasma membrane subdomains called lipid rafts	627:671	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	9	21	theme	GLUT1	1634:1638	arg1	density					1640:1646	GLUT1 density	1634:1646	GLUT1 density in membrane fractions	1634:1668	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	1	22	from	metabolite	136:145	arg1	cells					165:169	most mammalian cells	150:169	most mammalian cells	150:169	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	3	23	theme	therapeutic	517:527	arg1	strategies					529:538	therapeutic strategies	517:538	therapeutic strategies	517:538	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	1	24	theme	proper	176:181	arg1	regulation					183:192	proper regulation	176:192	proper regulation of uptake	176:202	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	10	25	theme	acute	1801:1805	arg1	activation					1807:1816	acute activation	1801:1816	acute activation in L929 cell	1801:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	5	26	theme	fibroblast	774:783	arg1	cells					785:789	L929 mouse fibroblast cells	763:789	L929 mouse fibroblast cells	763:789	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	9	27	contain	had	1617:1619	arg2	impact					1624:1629	no impact	1621:1629	no impact	1621:1629	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	9	27	contain	had	1617:1619	arg1	inhibition					1587:1596	pharmacologic inhibition	1573:1596	pharmacologic inhibition of these processes	1573:1615	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	7	28	dep	myriocin	1224:1231	arg1	treatment					1254:1262	treatment	1254:1262	treatment	1254:1262	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	10	29	from	fractions	1776:1784	arg1	response					1789:1796	response	1789:1796	response to acute activation in L929 cell	1789:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	5	30	theme	distinct	863:870	arg1	microdomain					851:861	a low density lipid microdomain	831:861	a low density lipid microdomain distinct from traditionally-defined lipid rafts	831:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	5	31	theme	traditionally-defined	877:897	arg1	rafts					905:909	traditionally-defined lipid rafts	877:909	traditionally-defined lipid rafts	877:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	5	32	theme	density	837:843	arg1	microdomain					851:861	a low density lipid microdomain	831:861	a low density lipid microdomain distinct from traditionally-defined lipid rafts	831:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	10	33	theme	membrane	1767:1774	arg1	fractions					1776:1784	low density membrane fractions	1755:1784	low density membrane fractions in response to acute activation in L929 cell	1755:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	5	34	from	rafts	905:909	arg1	distinct					863:870	distinct	863:870	distinct	863:870	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	2	35	theme	glucose	295:301	arg1	uptake					303:308	glucose uptake	295:308	glucose uptake in a wide variety of cells	295:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	3	36	theme	GLUT1	431:435	arg1	regulation					437:446	GLUT1 regulation	431:446	GLUT1 regulation within its various cellular environments	431:487	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	9	37	theme	processes	1607:1615	arg1	inhibition					1587:1596	pharmacologic inhibition	1573:1596	pharmacologic inhibition of these processes	1573:1615	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	8	38	theme	actin	1366:1370	arg1	cytoskeleton					1372:1383	the actin cytoskeleton	1362:1383	the actin cytoskeleton	1362:1383	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	9	39	from	density	1640:1646	arg1	fractions					1660:1668	membrane fractions	1651:1668	membrane fractions	1651:1668	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	3	40	theme	regulation	437:446	arg1	essential					492:500	essential	492:500	essential	492:500	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	3	40	theme	regulation	437:446	arg1	understanding					414:426	a better understanding	405:426	a better understanding of GLUT1 regulation within its various cellular environments	405:487	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	3	41	theme	cellular	467:474	arg1	environments					476:487	its various cellular environments	455:487	its various cellular environments	455:487	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	7	42	theme	membrane	1092:1099	arg1	microdomains					1101:1112	the GLUT1-containing membrane microdomains	1071:1112	the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity,	1071:1162	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	7	42	theme	membrane	1092:1099	arg1	sphingolipid-dependent					1176:1197	sphingolipid-dependent	1176:1197	sphingolipid-dependent	1176:1197	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	4	43	theme	lipid	661:665	arg1	rafts					667:671	lipid rafts	661:671	lipid rafts	661:671	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	2	44	theme	specific	377:384	arg1	manner					386:391	a tissue specific manner	368:391	a tissue specific manner	368:391	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	0	45	theme	sphingolipid-organized	25:46	arg1	domains					73:79	sphingolipid-organized, cholesterol-independent domains	25:79	sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells	25:110	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	7	46	from	microdomains	1101:1112	arg1	L929 cells					1117:1126	L929 cells	1117:1126	L929 cells	1117:1126	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	8	47	theme	lipid	1326:1330	arg1	composition					1332:1342	their lipid composition	1320:1342	their lipid composition	1320:1342	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	8	48	theme	GLUT1	1435:1439	arg1	association					1420:1430	the association	1416:1430	the association of GLUT1 with them	1416:1449	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	5	49	theme	lipid	845:849	arg1	microdomain					851:861	a low density lipid microdomain	831:861	a low density lipid microdomain distinct from traditionally-defined lipid rafts	831:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	5	50	theme	lipid	899:903	arg1	rafts					905:909	traditionally-defined lipid rafts	877:909	traditionally-defined lipid rafts	877:909	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	8	51	with	association	1420:1430	arg1	them					1446:1449	them	1446:1449	them	1446:1449	These microdomains appear to be organized primarily by their lipid composition, as disruption of the actin cytoskeleton or microtubules does not alter the association of GLUT1 with them.					
30980844	3	52	theme	glucose	575:581	arg1	homeostasis					583:593	glucose homeostasis	575:593	glucose homeostasis	575:593	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	9	53	with	association	1469:1479	arg1	microdomains					1501:1512	these microdomains	1495:1512	these microdomains	1495:1512	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	3	54	theme	various	459:465	arg1	environments					476:487	its various cellular environments	455:487	its various cellular environments	455:487	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	10	55	theme	density	1759:1765	arg1	fractions					1776:1784	low density membrane fractions	1755:1784	low density membrane fractions in response to acute activation in L929 cell	1755:1829	Importantly, we find no evidence that GLUT1 is actively translocated into or out of low density membrane fractions in response to acute activation in L929 cell.					
30980844	6	56	theme	cholesterol	948:958	arg1	removal					960:966	cholesterol removal	948:966	cholesterol removal	948:966	These structures are not altered by cholesterol removal with methyl-β-cyclodextrin and lack resistance to cold Triton X-100 extraction.					
30980844	7	57	theme	GLUT1-containing	1075:1090	arg1	microdomains					1101:1112	the GLUT1-containing membrane microdomains	1071:1112	the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity,	1071:1162	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	7	57	theme	GLUT1-containing	1075:1090	arg1	sphingolipid-dependent					1176:1197	sphingolipid-dependent	1176:1197	sphingolipid-dependent	1176:1197	Our data indicate that the GLUT1-containing membrane microdomains in L929 cells, as well as GLUT1's basal activity, are instead sphingolipid-dependent, being sensitive to both myriocin and sphingomyelinase treatment.					
30980844	0	58	dep	sphingolipid-organized	25:46	arg1	cholesterol-independent					49:71	cholesterol-independent	49:71	cholesterol-independent	49:71	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	1	59	theme	preferred	126:134	arg1	metabolite					136:145	a preferred metabolite	124:145	a preferred metabolite in most mammalian cells	124:169	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	1	59	theme	preferred	126:134	arg1	Glucose					113:119	Glucose	113:119	Glucose	113:119	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	2	60	theme	wide	315:318	arg1	variety					320:326	a wide variety	313:326	a wide variety of cells	313:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	2	60	theme	wide	315:318	arg1	cells					331:335	cells	331:335	cells	331:335	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	1	61	theme	organism	220:227	arg1	homeostasis					229:239	organism homeostasis	220:239	organism homeostasis	220:239	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	6	62	theme	cold	1018:1021	arg1	extraction					1036:1045	cold Triton X-100 extraction	1018:1045	cold Triton X-100 extraction	1018:1045	These structures are not altered by cholesterol removal with methyl-β-cyclodextrin and lack resistance to cold Triton X-100 extraction.					
30980844	4	63	theme	activity	719:726	arg1	regulation					692:701	regulation	692:701	regulation of GLUT1 uptake activity	692:726	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	9	64	theme	GLUT1	1484:1488	arg1	association					1469:1479	the association	1465:1479	the association of GLUT1 with these microdomains	1465:1512	Furthermore, the association of GLUT1 with these microdomains appears not to require palmitoylation or glycosylation, as pharmacologic inhibition of these processes had no impact on GLUT1 density in membrane fractions.					
30980844	0	65	theme	L929	84:87	arg1	cells					106:110	L929 mouse fibroblast cells	84:110	L929 mouse fibroblast cells	84:110	GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.					
30980844	1	66	theme	uptake	197:202	arg1	regulation					183:192	proper regulation	176:192	proper regulation of uptake	176:202	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	1	67	theme	most	150:153	arg1	cells					165:169	most mammalian cells	150:169	most mammalian cells	150:169	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	3	68	theme	better	407:412	arg1	essential					492:500	essential	492:500	essential	492:500	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	3	68	theme	better	407:412	arg1	understanding					414:426	a better understanding	405:426	a better understanding of GLUT1 regulation within its various cellular environments	405:487	Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis.					
30980844	4	69	theme	plasma	627:632	arg1	subdomains					643:652	plasma membrane subdomains	627:652	plasma membrane subdomains called lipid rafts	627:671	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
30980844	5	70	theme	L929	763:766	arg1	cells					785:789	L929 mouse fibroblast cells	763:789	L929 mouse fibroblast cells	763:789	While studying this phenomenon in L929 mouse fibroblast cells, we observed that GLUT1 associates with a low density lipid microdomain distinct from traditionally-defined lipid rafts.					
30980844	2	71	theme	glucose	246:252	arg1	transporter					254:264	The glucose transporter 1	242:266	The glucose transporter 1 (GLUT1)	242:274	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	2	71	theme	glucose	246:252	arg1	GLUT1					269:273	GLUT1	269:273	GLUT1	269:273	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	2	71	theme	glucose	246:252	arg1	responsible					279:289	responsible	279:289	responsible	279:289	The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner.					
30980844	1	72	theme	mammalian	155:163	arg1	cells					165:169	most mammalian cells	150:169	most mammalian cells	150:169	Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis.					
30980844	4	73	theme	GLUT1	706:710	arg1	activity					719:726	GLUT1 uptake activity	706:726	GLUT1 uptake activity	706:726	Previous findings suggest that plasma membrane subdomains called lipid rafts may play a role in regulation of GLUT1 uptake activity.					
31386359	7	0	theme	glycosyl	1138:1145	arg1	donor					1147:1151	the glycosyl donor	1134:1151	the glycosyl donor	1134:1151	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	2	1	theme	peptidoglycan	351:363	arg1	glycosyltransferases					365:384	peptidoglycan glycosyltransferases	351:384	peptidoglycan glycosyltransferases	351:384	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	0	2	theme	Elongation	62:71	arg1	Growth					19:24	Chain Growth	13:24	Chain Growth	13:24	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	0	2	theme	Elongation	62:71	arg1	Preferences					40:50	Substrate Preferences	30:50	Substrate Preferences	30:50	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	4	3	from	essential	750:758	arg1	bacteria					774:781	nearly all bacteria	763:781	nearly all bacteria	763:781	Because RodA and FtsW are essential in nearly all bacteria, these enzymes are promising targets for new antibiotics.					
31386359	6	4	theme	growing	1036:1042	arg1	chain					1051:1055	the growing glycan chain	1032:1055	the growing glycan chain	1032:1055	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	7	5	theme	distinct	1202:1209	arg1	requirements					1217:1228	distinct lipid requirements	1202:1228	distinct lipid requirements	1202:1228	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	6	6	theme	glycan	1044:1049	arg1	chain					1051:1055	the growing glycan chain	1032:1055	the growing glycan chain	1032:1055	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	8	7	theme	biochemical	1365:1375	arg1	studies					1392:1398	future biochemical and structural studies	1358:1398	future biochemical and structural studies	1358:1398	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	2	8	theme	glycopeptide	294:305	arg1	Lipid					318:322	a glycopeptide precursor, Lipid II	292:325	Lipid	318:322	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	2	9	gly	glycopeptide	294:305	arg2	glycopeptide					294:305	glycopeptide	294:305	glycopeptide	294:305	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	3	10	theme	Lipid	559:563	arg1	II					565:566	Lipid II	559:566	Lipid II	559:566	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	11	dep	proteins	657:664	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	11	dep	proteins	657:664	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	11	dep	proteins	657:664	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	0	12	theme	Sporulation-Family	88:105	arg1	Glycosyltransferases					121:140	Sporulation-Family Peptidoglycan Glycosyltransferases	88:140	Sporulation-Family Peptidoglycan Glycosyltransferases	88:140	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	3	13	theme	peptidoglycan	697:709	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	13	theme	peptidoglycan	697:709	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	14	theme	enzymes	535:541	arg1	family					525:530	only one family	516:530	only one family of enzymes that polymerize Lipid II	516:566	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	6	15	theme	SEDS	938:941	arg1	proteins					943:950	SEDS proteins	938:950	SEDS proteins	938:950	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	3	16	contain	contain	508:514	arg2	family					525:530	only one family	516:530	only one family of enzymes that polymerize Lipid II	516:566	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	16	contain	contain	508:514	arg1	bacteria					483:490	bacteria	483:490	bacteria	483:490	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	4	17	theme	promising	802:810	arg1	enzymes					790:796	these enzymes	784:796	these enzymes	784:796	Because RodA and FtsW are essential in nearly all bacteria, these enzymes are promising targets for new antibiotics.					
31386359	4	17	theme	promising	802:810	arg1	targets					812:818	promising targets	802:818	promising targets for new antibiotics	802:838	Because RodA and FtsW are essential in nearly all bacteria, these enzymes are promising targets for new antibiotics.					
31386359	6	18	theme	new	987:989	arg1	monomers					1000:1007	new Lipid II monomers	987:1007	new Lipid II monomers	987:1007	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	4	19	theme	new	824:826	arg1	antibiotics					828:838	new antibiotics	824:838	new antibiotics	824:838	Because RodA and FtsW are essential in nearly all bacteria, these enzymes are promising targets for new antibiotics.					
31386359	8	20	theme	polymerases	1319:1329	arg1	mechanism					1294:1302	the mechanism	1290:1302	the mechanism of SEDS-family polymerases	1290:1329	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	7	21	contain	have	1197:1200	arg2	requirements					1217:1228	distinct lipid requirements	1202:1228	distinct lipid requirements	1202:1228	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	7	21	contain	have	1197:1200	arg1	donor					1147:1151	the glycosyl donor	1134:1151	the glycosyl donor	1134:1151	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	7	21	contain	have	1197:1200	arg1	acceptor					1157:1164	acceptor	1157:1164	acceptor	1157:1164	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	2	22	theme	cell	460:463	arg1	wall					465:468	the mature cell wall	449:468	the mature cell wall	449:468	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	8	23	theme	first	1258:1262	arg1	insights					1276:1283	the first fundamental insights	1254:1283	the first fundamental insights into the mechanism of SEDS-family polymerases	1254:1329	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	5	24	theme	polymerases	904:914	arg1	mechanisms					884:893	the mechanisms	880:893	the mechanisms of these polymerases	880:914	However, almost nothing is known about the mechanisms of these polymerases.					
31386359	2	25	theme	glycan	391:396	arg1	strands					398:404	glycan strands	391:404	glycan strands that are subsequently cross-linked to form the mature cell wall	391:468	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	2	26	theme	mature	453:458	arg1	wall					465:468	the mature cell wall	449:468	the mature cell wall	449:468	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	0	27	theme	Glycosyltransferases	121:140	arg1	Growth					19:24	Chain Growth	13:24	Chain Growth	13:24	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	0	27	theme	Glycosyltransferases	121:140	arg1	Preferences					40:50	Substrate Preferences	30:50	Substrate Preferences	30:50	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	8	28	theme	fundamental	1264:1274	arg1	insights					1276:1283	the first fundamental insights	1254:1283	the first fundamental insights into the mechanism of SEDS-family polymerases	1254:1329	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	0	29	theme	Chain	13:17	arg1	Growth					19:24	Chain Growth	13:24	Chain Growth	13:24	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	0	30	theme	Peptidoglycan	107:119	arg1	Glycosyltransferases					121:140	Sporulation-Family Peptidoglycan Glycosyltransferases	88:140	Sporulation-Family Peptidoglycan Glycosyltransferases	88:140	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	3	31	theme	Sporulation	631:641	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	31	theme	Sporulation	631:641	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	31	theme	Sporulation	631:641	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	31	theme	Sporulation	631:641	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	2	32	theme	precursor	307:315	arg1	Lipid					318:322	a glycopeptide precursor, Lipid II	292:325	Lipid	318:322	Peptidoglycan is assembled from a glycopeptide precursor, Lipid II, that is polymerized by peptidoglycan glycosyltransferases into glycan strands that are subsequently cross-linked to form the mature cell wall.					
31386359	7	33	theme	polymerization	1173:1186	arg1	reaction					1188:1195	the polymerization reaction	1169:1195	the polymerization reaction	1169:1195	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	0	34	theme	Substrate	30:38	arg1	Preferences					40:50	Substrate Preferences	30:50	Substrate Preferences	30:50	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	3	35	theme	ubiquitous	587:596	arg1	Shape					598:602	ubiquitous Shape	587:602	ubiquitous Shape	587:602	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	6	36	theme	reducing	1016:1023	arg1	end					1025:1027	the reducing end	1012:1027	the reducing end of the growing glycan chain	1012:1055	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	0	37	theme	Growth	19:24	arg1	Direction					0:8	Direction	0:8	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.	0:141	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	6	38	theme	II	997:998	arg1	monomers					1000:1007	new Lipid II monomers	987:1007	new Lipid II monomers	987:1007	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	8	39	theme	future	1358:1363	arg1	studies					1392:1398	future biochemical and structural studies	1358:1398	future biochemical and structural studies	1358:1398	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	8	40	theme	SEDS-family	1307:1317	arg1	polymerases					1319:1329	SEDS-family polymerases	1307:1329	SEDS-family polymerases	1307:1329	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	7	41	theme	lipid	1211:1215	arg1	requirements					1217:1228	distinct lipid requirements	1202:1228	distinct lipid requirements	1202:1228	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	0	42	theme	Preferences	40:50	arg1	Direction					0:8	Direction	0:8	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.	0:141	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	3	43	theme	-family	649:655	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	43	theme	-family	649:655	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	43	theme	-family	649:655	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	43	theme	-family	649:655	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	8	44	theme	structural	1381:1390	arg1	studies					1392:1398	future biochemical and structural studies	1358:1398	future biochemical and structural studies	1358:1398	These findings provide the first fundamental insights into the mechanism of SEDS-family polymerases and lay the groundwork for future biochemical and structural studies.					
31386359	7	45	theme	reducing	1102:1109	arg1	end					1111:1113	the reducing end	1098:1113	the reducing end	1098:1113	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	7	46	from	donor	1147:1151	arg1	reaction					1188:1195	the polymerization reaction	1169:1195	the polymerization reaction	1169:1195	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	3	47	theme	Shape	598:602	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	47	theme	Shape	598:602	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	47	theme	Shape	598:602	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	47	theme	Shape	598:602	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	6	48	theme	Lipid	991:995	arg1	monomers					1000:1007	new Lipid II monomers	987:1007	new Lipid II monomers	987:1007	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	3	49	theme	Division	617:624	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	49	theme	Division	617:624	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	49	theme	Division	617:624	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	49	theme	Division	617:624	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	4	50	from	bacteria	774:781	arg1	essential					750:758	essential	750:758	essential	750:758	Because RodA and FtsW are essential in nearly all bacteria, these enzymes are promising targets for new antibiotics.					
31386359	6	51	theme	chain	1051:1055	arg1	end					1025:1027	the reducing end	1012:1027	the reducing end of the growing glycan chain	1012:1055	Here, we report that SEDS proteins synthesize peptidoglycan by adding new Lipid II monomers to the reducing end of the growing glycan chain.					
31386359	1	52	theme	established	224:234	arg1	target					236:241	an established target	221:241	an established target for antibiotics	221:257	The bacterial cell wall is composed of peptidoglycan, and its biosynthesis is an established target for antibiotics.					
31386359	1	52	theme	established	224:234	arg1	biosynthesis					205:216	its biosynthesis	201:216	its biosynthesis	201:216	The bacterial cell wall is composed of peptidoglycan, and its biosynthesis is an established target for antibiotics.					
31386359	7	53	from	acceptor	1157:1164	arg1	reaction					1188:1195	the polymerization reaction	1169:1195	the polymerization reaction	1169:1195	Using substrates that can only react at the reducing end, we also show that the glycosyl donor and acceptor in the polymerization reaction have distinct lipid requirements.					
31386359	1	54	theme	bacterial	147:155	arg1	wall					162:165	The bacterial cell wall	143:165	The bacterial cell wall	143:165	The bacterial cell wall is composed of peptidoglycan, and its biosynthesis is an established target for antibiotics.					
31386359	0	55	theme	Shape	55:59	arg1	Growth					19:24	Chain Growth	13:24	Chain Growth	13:24	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	0	55	theme	Shape	55:59	arg1	Preferences					40:50	Substrate Preferences	30:50	Substrate Preferences	30:50	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	3	56	theme	Elongation	605:614	arg1	FtsW					675:678	FtsW	675:678	FtsW	675:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	56	theme	Elongation	605:614	arg1	RodA					666:669	RodA	666:669	RodA	666:669	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	56	theme	Elongation	605:614	arg1	polymerases					711:721	peptidoglycan polymerases	697:721	peptidoglycan polymerases	697:721	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	3	56	theme	Elongation	605:614	arg1	proteins					657:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins	583:664	the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW	583:678	For decades bacteria were thought to contain only one family of enzymes that polymerize Lipid II, but recently, the ubiquitous Shape, Elongation, Division, and Sporulation (SEDS)-family proteins RodA and FtsW were shown to be peptidoglycan polymerases.					
31386359	1	57	theme	cell	157:160	arg1	wall					162:165	The bacterial cell wall	143:165	The bacterial cell wall	143:165	The bacterial cell wall is composed of peptidoglycan, and its biosynthesis is an established target for antibiotics.					
31386359	0	58	theme	Division	74:81	arg1	Growth					19:24	Chain Growth	13:24	Chain Growth	13:24	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31386359	0	58	theme	Division	74:81	arg1	Preferences					40:50	Substrate Preferences	30:50	Substrate Preferences	30:50	Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases.					
31672634	4	0	theme	antioxidant	899:909	arg1	activities					911:920	The antioxidant activities	895:920	The antioxidant activities of composite films	895:939	The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro.					
31672634	1	1	theme	Falkenbergia	321:332	arg1	rufolanosa					334:343	red marine alga Falkenbergia rufolanosa	305:343	red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	305:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	6	2	theme	antioxidant	1452:1462	arg1	material					1464:1471	a potent antioxidant material	1443:1471	a potent antioxidant material	1443:1471	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	1	3	theme	wound	505:509	arg1	healing					511:517	the potential wound healing	491:517	the potential wound healing	491:517	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	4	theme	different	404:412	arg1	ratios					414:419	different ratios	404:419	different ratios of FRP/PVA	404:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	5	from	ratios	414:419	arg1	films					395:399	poly (vinyl alcohol) (PVA) composed films	359:399	poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	359:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	3	6	theme	glass	766:770	arg1	transition					772:781	glass transition	766:781	glass transition	766:781	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	1	7	theme	morphological	241:253	arg1	properties					255:264	physicochemical, antioxidant and morphological properties	208:264	physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	208:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	0	8	theme	promising	80:88	arg1	agent					104:108	A promising wound healing agent	78:108	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	5	9	theme	higher	1160:1165	arg1	content					1167:1173	a higher content	1158:1173	a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups	1158:1320	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	6	10	theme	biodegradable	1420:1432	arg1	films					1434:1438	biodegradable films	1420:1438	biodegradable films as a potent antioxidant material	1420:1471	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	1	11	dep	F1	433:434	arg1	effects					519:525	effects	519:525	effects	519:525	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	6	12	theme	substantial	1378:1388	arg1	pledge					1390:1395	a substantial pledge	1376:1395	a substantial pledge for the development of biodegradable films as a potent antioxidant material	1376:1471	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	3	13	theme	PVA	883:885	arg1	ratios					887:892	PVA ratios	883:892	PVA ratios	883:892	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	5	14	dep	collagen	1178:1185	arg1	885.12 ± 20.35 mg/g					1188:1206	885.12 ± 20.35 mg/g	1188:1206	885.12 ± 20.35 mg/g of tissue	1188:1216	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	6	15	theme	films	1434:1438	arg1	development					1405:1415	the development	1401:1415	the development of biodegradable films as a potent antioxidant material	1401:1471	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	5	16	theme	histological	1232:1243	arg1	examination					1245:1255	histological examination	1232:1255	histological examination	1232:1255	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	3	17	theme	semi-crystalline	802:817	arg1	structure					819:827	a semi-crystalline structure	800:827	a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios	800:892	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	0	18	theme	healing	96:102	arg1	agent					104:108	A promising wound healing agent	78:108	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	1	19	theme	poly	359:362	arg1	films					395:399	poly (vinyl alcohol) (PVA) composed films	359:399	poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	359:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	20	theme	FRP/PVA	424:430	arg1	ratios					414:419	different ratios	404:419	different ratios of FRP/PVA	404:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	4	21	theme	antioxidant	980:990	arg1	activity					992:999	the highest antioxidant activity	968:999	the highest antioxidant activity	968:999	The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro.					
31672634	0	22	theme	wound	90:94	arg1	agent					104:108	A promising wound healing agent	78:108	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	2	23	with	heterogeneous	569:581	arg1	surface					613:619	a rough surface	605:619	a rough surface	605:619	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	5	24	theme	higher	1123:1128	arg1	scores					1147:1152	higher wound appearance scores	1123:1152	higher wound appearance scores	1123:1152	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	0	25	theme	rufolanosa	13:22	arg1	polysaccharide					24:37	Falkenbergia rufolanosa polysaccharide	0:37	Falkenbergia rufolanosa polysaccharide	0:37	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	1	26	theme	vinyl	365:369	arg1	poly					359:362	poly	359:362	poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	359:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	26	theme	vinyl	365:369	arg1	alcohol					371:377	vinyl alcohol	365:377	vinyl alcohol	365:377	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	3	27	theme	transition	772:781	arg1	results					783:789	X-ray diffraction and glass transition results	744:789	X-ray diffraction and glass transition results	744:789	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	6	28	theme	potent	1445:1450	arg1	antioxidant					1452:1462	a potent antioxidant	1443:1462	a potent antioxidant material	1443:1471	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	0	29	theme	Falkenbergia	0:11	arg1	polysaccharide					24:37	Falkenbergia rufolanosa polysaccharide	0:37	Falkenbergia rufolanosa polysaccharide	0:37	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	1	30	theme	polysaccharide	269:282	arg1	properties					255:264	physicochemical, antioxidant and morphological properties	208:264	physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	208:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	3	31	theme	X-ray	744:748	arg1	diffraction					750:760	X-ray diffraction	744:760	X-ray diffraction	744:760	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	2	32	theme	prepared	549:556	arg1	films					558:562	FRP/PVA prepared films	541:562	FRP/PVA prepared films	541:562	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	0	33	dep	films	71:75	arg1	agent					104:108	A promising wound healing agent	78:108	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	3	34	theme	films	845:849	arg1	structure					819:827	a semi-crystalline structure	800:827	a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios	800:892	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	5	35	theme	appearance	1136:1145	arg1	scores					1147:1152	higher wound appearance scores	1123:1152	higher wound appearance scores	1123:1152	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	3	36	theme	diffraction	750:760	arg1	results					783:789	X-ray diffraction and glass transition results	744:789	X-ray diffraction and glass transition results	744:789	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	2	37	dep	heterogeneous	569:581	arg1	opaque					593:598	opaque	593:598	opaque	593:598	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	0	38	theme	polysaccharide	24:37	arg1	films					71:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films	0:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	4	39	theme	highest	972:978	arg1	activity					992:999	the highest antioxidant activity	968:999	the highest antioxidant activity	968:999	The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro.					
31672634	3	40	theme	composed	836:843	arg1	films					845:849	FRP composed films	832:849	FRP composed films	832:849	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	3	41	theme	FRP	832:834	arg1	films					845:849	FRP composed films	832:849	FRP composed films	832:849	Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios.					
31672634	5	42	theme	tissue	1211:1216	arg1	885.12 ± 20.35 mg/g					1188:1206	885.12 ± 20.35 mg/g	1188:1206	885.12 ± 20.35 mg/g of tissue	1188:1216	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	5	43	theme	treatment	1099:1107	arg1	days					1091:1094	eight days	1085:1094	eight days of treatment	1085:1107	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	0	44	theme	laser	125:129	arg1	burns					131:135	dermal laser burns	118:135	dermal laser burns	118:135	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	1	45	theme	%	480:480	arg1	PVA					482:484	100% PVA	477:484	100% PVA	477:484	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	45	theme	%	480:480	arg1	PVA					472:474	PVA	472:474	PVA (100% PVA)	472:485	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	0	46	theme	Poly	41:44	arg1	films					71:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films	0:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	4	47	theme	composite	925:933	arg1	films					935:939	composite films	925:939	composite films	925:939	The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro.					
31672634	0	48	theme	dermal	118:123	arg1	burns					131:135	dermal laser burns	118:135	dermal laser burns	118:135	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	6	49	theme	tissue	1499:1504	arg1	regeneration					1506:1517	tissue regeneration	1499:1517	tissue regeneration	1499:1517	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	1	50	dep	conducted	160:168	arg1	PVA					472:474	PVA	472:474	PVA (100% PVA)	472:485	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	F3					457:458	F3	457:458	F3	457:458	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	F2					445:446	F2	445:446	F2 (50:50)	445:454	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	30:70					461:465	30:70	461:465	30:70	461:465	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	F1					433:434	F1	433:434	F1 (70:30)	433:442	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	PVA					482:484	100% PVA	477:484	100% PVA	477:484	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	50:50					449:453	50:50	449:453	50:50	449:453	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	50	dep	conducted	160:168	arg1	70:30					437:441	70:30	437:441	70:30	437:441	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	5	51	theme	CYTOL	1286:1290	arg1	BASIC®					1292:1297	CYTOL BASIC®	1286:1297	CYTOL BASIC®	1286:1297	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	4	52	theme	films	935:939	arg1	activities					911:920	The antioxidant activities	895:920	The antioxidant activities of composite films	895:939	The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro.					
31672634	5	53	theme	wound	1064:1068	arg1	healing					1070:1076	the wound healing	1060:1076	the wound healing	1060:1076	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	2	54	dep	transform	647:655	arg1	infrared					657:664	infrared	657:664	transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters	647:735	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	6	55	theme	marine-derived	1341:1354	arg1	All					1323:1325	All	1323:1325	All	1323:1325	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	6	55	theme	marine-derived	1341:1354	arg1	polysaccharide					1356:1369	the marine-derived polysaccharide	1337:1369	the marine-derived polysaccharide	1337:1369	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	0	56	theme	vinyl	46:50	arg1	Poly					41:44	Poly	41:44	Poly(vinyl alcohol)	41:59	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	0	56	theme	vinyl	46:50	arg1	alcohol					52:58	vinyl alcohol	46:58	vinyl alcohol	46:58	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	1	57	theme	red	305:307	arg1	rufolanosa					334:343	red marine alga Falkenbergia rufolanosa	305:343	red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	305:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	5	58	theme	collagen	1178:1185	arg1	content					1167:1173	a higher content	1158:1173	a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups	1158:1320	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	5	58	theme	collagen	1178:1185	arg1	scores					1147:1152	higher wound appearance scores	1123:1152	higher wound appearance scores	1123:1152	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	2	59	dep	prepared	549:556	arg1	FRP/PVA					541:547	FRP/PVA	541:547	FRP/PVA	541:547	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	1	60	theme	marine	309:314	arg1	rufolanosa					334:343	red marine alga Falkenbergia rufolanosa	305:343	red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	305:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	61	theme	composed	386:393	arg1	films					395:399	poly (vinyl alcohol) (PVA) composed films	359:399	poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	359:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	5	62	theme	PVA-treated	1303:1313	arg1	groups					1315:1320	PVA-treated groups	1303:1320	PVA-treated groups	1303:1320	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	6	63	theme	promising	1479:1487	arg1	agent					1489:1493	a promising agent	1477:1493	a promising agent for tissue regeneration	1477:1517	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	5	64	theme	wound	1130:1134	arg1	scores					1147:1152	higher wound appearance scores	1123:1152	higher wound appearance scores	1123:1152	Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12 ± 20.35 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups.					
31672634	6	65	link	marine-derived	1341:1354	arg1	All					1323:1325	All	1323:1325	All	1323:1325	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	6	65	link	marine-derived	1341:1354	arg1	polysaccharide					1356:1369	the marine-derived polysaccharide	1337:1369	the marine-derived polysaccharide	1337:1369	All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.					
31672634	2	66	theme	electron	689:696	arg1	microscopy					698:707	scanning electron microscopy	680:707	scanning electron microscopy	680:707	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	1	67	theme	physicochemical	208:222	arg1	properties					255:264	physicochemical, antioxidant and morphological properties	208:264	physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	208:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	0	68	theme	composite	61:69	arg1	films					71:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films	0:75	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.	0:144	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31672634	2	69	theme	colorimetric	713:724	arg1	parameters					726:735	colorimetric parameters	713:735	colorimetric parameters	713:735	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	2	70	theme	rough	607:611	arg1	surface					613:619	a rough surface	605:619	a rough surface	605:619	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	2	71	theme	scanning	680:687	arg1	microscopy					698:707	scanning electron microscopy	680:707	scanning electron microscopy	680:707	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	1	72	theme	alga	316:319	arg1	rufolanosa					334:343	red marine alga Falkenbergia rufolanosa	305:343	red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	305:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	1	73	theme	antioxidant	225:235	arg1	properties					255:264	physicochemical, antioxidant and morphological properties	208:264	physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA	208:430	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	2	74	dep	Fourier	639:645	arg1	transform					647:655	transform	647:655	transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters	647:735	As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters.					
31672634	1	75	theme	potential	495:503	arg1	healing					511:517	the potential wound healing	491:517	the potential wound healing	491:517	This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects.					
31672634	0	76	from	agent	104:108	arg1	rats					140:143	rats	140:143	rats	140:143	Falkenbergia rufolanosa polysaccharide - Poly(vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats.					
31252018	5	0	contain	had	794:796	arg1	factors					736:742	Environmental factors	722:742	Environmental factors such as temperature and relative humidities (RHs)	722:792	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	0	contain	had	794:796	arg1	temperature					752:762	temperature	752:762	temperature	752:762	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	0	contain	had	794:796	arg1	humidities					777:786	relative humidities	768:786	relative humidities (RHs)	768:792	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	0	contain	had	794:796	arg2	influences					810:819	significant influences	798:819	significant influences	798:819	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	6	1	theme	bioactive	985:993	arg1	components					995:1004	complex and bioactive components	973:1004	complex and bioactive components	973:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	4	2	theme	phenols	697:703	arg1	release					682:688	the release	678:688	the release of the phenols	678:703	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	2	theme	phenols	697:703	arg1	slowest					713:719	the slowest	709:719	the slowest	709:719	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	0	3	dep	composition	103:113	arg1	the					99:101	the	99:101	the	99:101	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	1	4	theme	important	236:244	arg1	polymers					259:266	important carbohydrate polymers	236:266	important carbohydrate polymers as wall materials	236:284	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	4	theme	important	236:244	arg1	maltodextrins					212:224	maltodextrins	212:224	maltodextrins (MDs)	212:230	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	4	theme	important	236:244	arg1	starch					201:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	5	5	theme	CACs	848:851	arg1	kinetics					836:843	the release kinetics	824:843	the release kinetics of CACs in REO	824:858	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	1	6	theme	carbohydrate	246:257	arg1	polymers					259:266	important carbohydrate polymers	236:266	important carbohydrate polymers as wall materials	236:284	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	6	theme	carbohydrate	246:257	arg1	maltodextrins					212:224	maltodextrins	212:224	maltodextrins (MDs)	212:230	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	6	theme	carbohydrate	246:257	arg1	starch					201:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	7	theme	Octenyl	159:165	arg1	anhydride					176:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	4	8	theme	characteristic	545:558	arg1	CACs					578:581	CACs	578:581	CACs	578:581	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	8	theme	characteristic	545:558	arg1	components					566:575	the seven characteristic aroma components	535:575	the seven characteristic aroma components (CACs) of rose essential oil (REO)	535:610	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	5	9	from	kinetics	836:843	arg1	REO					856:858	REO	856:858	REO	856:858	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	3	10	theme	essential	493:501	arg1	oils					503:506	essential oils	493:506	essential oils	493:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	1	11	theme	succinic	167:174	arg1	anhydride					176:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	6	12	theme	MDs	933:935	arg1	use					902:904	the use	898:904	the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components	898:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	2	13	theme	few	296:298	arg1	studies					300:306	few studies	296:306	few studies	296:306	However, few studies have shown whether these two wall materials affect the composition of core materials.					
31252018	4	14	theme	oil	602:604	arg1	CACs					578:581	CACs	578:581	CACs	578:581	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	14	theme	oil	602:604	arg1	components					566:575	the seven characteristic aroma components	535:575	the seven characteristic aroma components (CACs) of rose essential oil (REO)	535:610	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	5	15	theme	Environmental	722:734	arg1	factors					736:742	Environmental factors	722:742	Environmental factors such as temperature and relative humidities (RHs)	722:792	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	15	theme	Environmental	722:734	arg1	temperature					752:762	temperature	752:762	temperature	752:762	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	15	theme	Environmental	722:734	arg1	humidities					777:786	relative humidities	768:786	relative humidities (RHs)	768:792	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	6	16	theme	new	880:882	arg1	insights					884:891	new insights	880:891	new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components	880:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	5	17	theme	significant	798:808	arg1	influences					810:819	significant influences	798:819	significant influences	798:819	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	0	18	theme	octenyl	23:29	arg1	anhydride					40:48	octenyl succinic anhydride	23:48	octenyl succinic anhydride (OSA)	23:54	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	0	18	theme	octenyl	23:29	arg1	OSA					51:53	OSA	51:53	OSA	51:53	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	0	19	theme	release	119:125	arg1	property					127:134	release property	119:134	release property	119:134	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	4	20	theme	rose	587:590	arg1	REO					607:609	REO	607:609	REO	607:609	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	20	theme	rose	587:590	arg1	oil					602:604	rose essential oil	587:604	rose essential oil (REO)	587:610	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	1	21	theme	wall	271:274	arg1	materials					276:284	wall materials	271:284	wall materials	271:284	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	6	22	theme	wall	940:943	arg1	materials					945:953	wall materials	940:953	wall materials for encapsulating complex and bioactive components	940:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	5	23	theme	release	828:834	arg1	kinetics					836:843	the release kinetics	824:843	the release kinetics of CACs in REO	824:858	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	1	24	theme	-modified	191:199	arg1	polymers					259:266	important carbohydrate polymers	236:266	important carbohydrate polymers as wall materials	236:284	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	24	theme	-modified	191:199	arg1	maltodextrins					212:224	maltodextrins	212:224	maltodextrins (MDs)	212:230	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	1	24	theme	-modified	191:199	arg1	starch					201:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch	159:206	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	2	25	theme	materials	383:391	arg1	composition					363:373	the composition	359:373	the composition of core materials	359:391	However, few studies have shown whether these two wall materials affect the composition of core materials.					
31252018	0	26	theme	succinic	31:38	arg1	anhydride					40:48	octenyl succinic anhydride	23:48	octenyl succinic anhydride (OSA)	23:54	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	0	26	theme	succinic	31:38	arg1	OSA					51:53	OSA	51:53	OSA	51:53	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	5	27	theme	relative	768:775	arg1	RHs					789:791	RHs	789:791	RHs	789:791	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	5	27	theme	relative	768:775	arg1	humidities					777:786	relative humidities	768:786	relative humidities (RHs)	768:792	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	2	28	theme	core	378:381	arg1	materials					383:391	core materials	378:391	core materials	378:391	However, few studies have shown whether these two wall materials affect the composition of core materials.					
31252018	0	29	theme	essential	144:152	arg1	oil					154:156	rose essential oil	139:156	rose essential oil	139:156	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	0	30	theme	rose	139:142	arg1	oil					154:156	rose essential oil	139:156	rose essential oil	139:156	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	3	31	from	effects	428:434	arg1	property					481:488	the release property	469:488	the release property of essential oils	469:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	3	32	theme	MD	463:464	arg1	effects					428:434	the effects	424:434	the effects of OSA-modified starch and MD on the release property of essential oils	424:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	5	33	from	REO	856:858	arg1	kinetics					836:843	the release kinetics	824:843	the release kinetics of CACs in REO	824:858	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	1	34	dep	-modified	191:199	arg1	anhydride					176:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride	159:184	Octenyl succinic anhydride (OSA)-modified starch and maltodextrins (MDs) are important carbohydrate polymers as wall materials.					
31252018	3	35	theme	OSA-modified	439:450	arg1	starch					452:457	OSA-modified starch	439:457	OSA-modified starch	439:457	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	0	36	theme	oil	154:156	arg1	property					127:134	release property	119:134	release property	119:134	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	0	36	theme	oil	154:156	arg1	composition					103:113	composition	103:113	composition	103:113	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	3	37	theme	oils	503:506	arg1	property					481:488	the release property	469:488	the release property of essential oils	469:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	3	38	theme	starch	452:457	arg1	effects					428:434	the effects	424:434	the effects of OSA-modified starch and MD on the release property of essential oils	424:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	5	39	from	CACs	848:851	arg1	REO					856:858	REO	856:858	REO	856:858	Environmental factors such as temperature and relative humidities (RHs) had significant influences on the release kinetics of CACs in REO.					
31252018	6	40	theme	starch	922:927	arg1	use					902:904	the use	898:904	the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components	898:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	0	41	theme	-modified	55:63	arg1	starch					65:70	octenyl succinic anhydride (OSA)-modified starch	23:70	octenyl succinic anhydride (OSA)-modified starch	23:70	Microcapsules based on octenyl succinic anhydride (OSA)-modified starch and maltodextrins changing the composition and release property of rose essential oil.					
31252018	4	42	theme	essential	592:600	arg1	REO					607:609	REO	607:609	REO	607:609	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	42	theme	essential	592:600	arg1	oil					602:604	rose essential oil	587:604	rose essential oil (REO)	587:610	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	3	43	theme	release	473:479	arg1	property					481:488	the release property	469:488	the release property of essential oils	469:506	In this work, we investigated the effects of OSA-modified starch and MD on the release property of essential oils.					
31252018	6	44	theme	complex	973:979	arg1	components					995:1004	complex and bioactive components	973:1004	complex and bioactive components	973:1004	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	6	45	theme	OSA-modified	909:920	arg1	starch					922:927	OSA-modified starch	909:927	OSA-modified starch	909:927	This work provides new insights into the use of OSA-modified starch and MDs as wall materials for encapsulating complex and bioactive components.					
31252018	4	46	theme	aroma	560:564	arg1	CACs					578:581	CACs	578:581	CACs	578:581	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	4	46	theme	aroma	560:564	arg1	components					566:575	the seven characteristic aroma components	535:575	the seven characteristic aroma components (CACs) of rose essential oil (REO)	535:610	Results showed that among the seven characteristic aroma components (CACs) of rose essential oil (REO), the esters released the fastest, followed by the alcohols, while the release of the phenols was the slowest.					
31252018	2	47	theme	wall	337:340	arg1	materials					342:350	these two wall materials	327:350	these two wall materials	327:350	However, few studies have shown whether these two wall materials affect the composition of core materials.					
29954478	7	0	dep	minerals	1074:1081	arg1	index					1322:1326	-differential refractive index	1297:1326	minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1074:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	0	dep	minerals	1074:1081	arg1	dRI					1329:1331	dRI	1329:1331	dRI	1329:1331	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	1	theme	amperometric	1152:1163	arg1	detection					1165:1173	pulsed amperometric detection	1145:1173	pulsed amperometric detection for free sugars	1145:1189	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	10	2	theme	components-organic	1700:1717	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	2	theme	components-organic	1700:1717	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	2	theme	components-organic	1700:1717	arg1	monosaccharides					1736:1750	monosaccharides	1736:1750	monosaccharides	1736:1750	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	2	theme	components-organic	1700:1717	arg1	minerals					1726:1733	minerals	1726:1733	minerals	1726:1733	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	1	3	theme	vera	240:243	arg1	plant					245:249	the Aloe vera plant	231:249	the Aloe vera plant	231:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	12	4	theme	ethanol-precipitated	1989:2008	arg1	products					2010:2017	ethanol-precipitated products	1989:2017	ethanol-precipitated products	1989:2017	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	15	5	theme	vera	2802:2805	arg1	products					2807:2814	the various commercially available A. vera products	2764:2814	the various commercially available A. vera products in this study	2764:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	2	6	from	differences	321:331	arg1	composition					336:346	composition	336:346	composition	336:346	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	15	7	dep	available	2789:2797	arg1	A.					2799:2800	A.	2799:2800	A.	2799:2800	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	8	theme	available	2789:2797	arg1	products					2807:2814	the various commercially available A. vera products	2764:2814	the various commercially available A. vera products in this study	2764:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	13	9	from	content	2291:2297	arg1	samples					2341:2347	the commercial aloe samples	2321:2347	the commercial aloe samples analyzed	2321:2356	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	0	10	theme	Chemical	0:7	arg1	Investigation					9:21	Chemical Investigation	0:21	Chemical Investigation of Major Constituents in Aloe vera	0:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	15	11	theme	differential	2636:2647	arg1	detectors					2652:2660	differential RI detectors	2636:2660	differential RI detectors	2636:2660	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	9	12	theme	major	1452:1456	arg1	constituents					1458:1469	The major constituents	1448:1469	The major constituents of A. vera fresh leaf	1448:1491	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	12	theme	major	1452:1456	arg1	Results					1439:1445	Results	1439:1445	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.	1439:1640	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	12	theme	major	1452:1456	arg1	fibers					1497:1502	fibers	1497:1502	fibers	1497:1502	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	15	13	theme	products	2807:2814	arg1	contents					2752:2759	contents	2752:2759	contents	2752:2759	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	13	theme	products	2807:2814	arg1	MW					2745:2746	polysaccharide MW	2730:2746	polysaccharide MW	2730:2746	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	4	14	theme	major	650:654	arg1	constituents					665:676	their major chemical constituents	644:676	their major chemical constituents	644:676	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	7	15	theme	high-performance	1084:1099	arg1	chromatography					1116:1129	high-performance anion-exchange chromatography	1084:1129	high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars	1084:1189	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	15	theme	high-performance	1084:1099	arg1	minerals					1074:1081	minerals	1074:1081	minerals	1074:1081	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	13	16	from	MW	2284:2285	arg1	samples					2341:2347	the commercial aloe samples	2321:2347	the commercial aloe samples analyzed	2321:2356	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	1	17	theme	scientific	168:177	arg1	literature					179:188	the scientific literature	164:188	the scientific literature reporting on the chemical composition of the Aloe vera plant	164:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	6	18	theme	free	938:941	arg1	lipids					905:910	lipids	905:910	lipids	905:910	The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides.					
29954478	6	18	theme	free	938:941	arg1	sugars					943:948	free sugars	938:948	free sugars	938:948	The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides.					
29954478	15	19	theme	improved	2682:2689	arg1	tool					2691:2694	an improved tool	2679:2694	an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study	2679:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	19	theme	improved	2682:2689	arg1	use					2598:2600	The use	2594:2600	The use of the SEC combined with MALS and differential RI detectors	2594:2660	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	4	20	theme	juice	608:612	arg1	powders					614:620	commercial aloe juice powders	592:620	commercial aloe juice powders	592:620	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	13	21	theme	processes	2418:2426	arg1	types					2395:2399	the types	2391:2399	the types of manufacturing processes employed	2391:2435	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	5	22	theme	fresh	696:700	arg1	powders					748:754	fresh leaves from China and Mexico, plus commercial powders	696:754	fresh leaves from China and Mexico, plus commercial powders	696:754	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	9	23	theme	vera	1477:1480	arg1	leaf					1488:1491	A. vera fresh leaf	1474:1491	A. vera fresh leaf	1474:1491	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	10	24	theme	total	1801:1805	arg1	composition					1807:1817	the total composition	1797:1817	the total composition	1797:1817	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	9	25	theme	leaf	1488:1491	arg1	constituents					1458:1469	The major constituents	1448:1469	The major constituents of A. vera fresh leaf	1448:1491	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	25	theme	leaf	1488:1491	arg1	Results					1439:1445	Results	1439:1445	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.	1439:1640	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	25	theme	leaf	1488:1491	arg1	fibers					1497:1502	fibers	1497:1502	fibers	1497:1502	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	12	26	theme	aloe	2113:2116	arg1	polysaccharides					2118:2132	aloe polysaccharides	2113:2132	aloe polysaccharides	2113:2132	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	7	27	theme	plasma-optical	1033:1046	arg1	spectroscopy					1057:1068	inductively coupled plasma-optical emission spectroscopy	1013:1068	inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1013:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	1	28	from	literature	179:188	arg1	number					144:149	a substantial number	130:149	a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant	130:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	10	29	theme	juice	1674:1678	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	29	theme	juice	1674:1678	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	5	30	theme	commercial	737:746	arg1	powders					748:754	fresh leaves from China and Mexico, plus commercial powders	696:754	fresh leaves from China and Mexico, plus commercial powders	696:754	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	1	31	from	papers	154:159	arg1	literature					179:188	the scientific literature	164:188	the scientific literature reporting on the chemical composition of the Aloe vera plant	164:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	9	32	theme	organic	1515:1521	arg1	acids					1523:1527	organic acids	1515:1527	organic acids	1515:1527	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	32	theme	organic	1515:1521	arg1	fibers					1497:1502	fibers	1497:1502	fibers	1497:1502	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	12	33	theme	manufacturing	2063:2075	arg1	conditions					2077:2086	manufacturing conditions	2063:2086	manufacturing conditions	2063:2086	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	1	34	from	number	144:149	arg1	literature					179:188	the scientific literature	164:188	the scientific literature reporting on the chemical composition of the Aloe vera plant	164:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	0	35	theme	Aloe	88:91	arg1	Powders					99:105	and Several Commercial Aloe Juice Powders	65:105	Powders	99:105	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	0	36	dep	Powders	99:105	arg1	Several					69:75	Several	69:75	Several	69:75	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	14	37	theme	derived	2576:2582	arg1	products					2584:2591	commercially derived products	2563:2591	commercially derived products	2563:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	11	38	theme	protein	1941:1947	arg1	fiber					1931:1935	fiber	1931:1935	fiber	1931:1935	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	38	theme	protein	1941:1947	arg1	amounts					1920:1926	high amounts	1915:1926	high amounts of fiber and protein	1915:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	38	theme	protein	1941:1947	arg1	protein					1941:1947	protein	1941:1947	protein	1941:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	8	39	theme	absolute	1367:1374	arg1	MW					1376:1377	The absolute MW	1363:1377	The absolute MW	1363:1377	The absolute MW and MW distribution were determined using MALS measurement.					
29954478	11	40	theme	high	1915:1918	arg1	fiber					1931:1935	fiber	1931:1935	fiber	1931:1935	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	40	theme	high	1915:1918	arg1	amounts					1920:1926	high amounts	1915:1926	high amounts of fiber and protein	1915:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	40	theme	high	1915:1918	arg1	protein					1941:1947	protein	1941:1947	protein	1941:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	5	41	theme	different	761:769	arg1	manufacturers					771:783	different manufacturers	761:783	different manufacturers made from different plant parts and/or manufacturing processes	761:846	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	7	42	theme	polysaccharide	1338:1351	arg1	analyses					1353:1360	polysaccharide analyses	1338:1360	polysaccharide analyses	1338:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	0	43	from	Constituents	32:43	arg1	vera					53:56	Aloe vera	48:56	Aloe vera	48:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	5	44	theme	plant	805:809	arg1	parts					811:815	different plant parts	795:815	different plant parts	795:815	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	7	45	dep	-multi-angle	1255:1266	arg1	chromatography					1235:1248	size exclusion chromatography	1220:1248	size exclusion chromatography	1220:1248	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	2	46	theme	available	422:430	arg1	forms					440:444	commercially available product forms	409:444	commercially available product forms	409:444	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	15	47	theme	MW	2745:2746	arg1	determination					2713:2725	the accurate determination	2700:2725	the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study	2700:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	7	48	theme	organic	1201:1207	arg1	acids					1209:1213	organic acids	1201:1213	organic acids	1201:1213	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	10	49	theme	powdered	1660:1667	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	49	theme	powdered	1660:1667	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	0	50	theme	Constituents	32:43	arg1	Investigation					9:21	Chemical Investigation	0:21	Chemical Investigation of Major Constituents in Aloe vera	0:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	13	51	theme	overall	2214:2220	arg1	profiles					2231:2238	The overall chemical profiles	2210:2238	The overall chemical profiles	2210:2238	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	13	51	theme	overall	2214:2220	arg1	consistent					2257:2266	consistent	2257:2266	consistent	2257:2266	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	15	52	theme	polysaccharide	2730:2743	arg1	MW					2745:2746	polysaccharide MW	2730:2746	polysaccharide MW	2730:2746	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	14	53	theme	leaf	2554:2557	arg1	composition					2531:2541	the major chemical composition	2512:2541	the major chemical composition of A. vera leaf and commercially derived products	2512:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	2	54	from	variations	378:387	arg1	fresh					392:396	fresh	392:396	fresh	392:396	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	2	54	from	variations	378:387	arg1	forms					440:444	commercially available product forms	409:444	commercially available product forms	409:444	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	7	55	theme	refractive	1311:1320	arg1	index					1322:1326	-differential refractive index	1297:1326	minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1074:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	55	theme	refractive	1311:1320	arg1	dRI					1329:1331	dRI	1329:1331	dRI	1329:1331	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	8	56	theme	MALS	1421:1424	arg1	measurement					1426:1436	MALS measurement	1421:1436	MALS measurement	1421:1436	The absolute MW and MW distribution were determined using MALS measurement.					
29954478	10	57	theme	major	1694:1698	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	57	theme	major	1694:1698	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	57	theme	major	1694:1698	arg1	monosaccharides					1736:1750	monosaccharides	1736:1750	monosaccharides	1736:1750	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	57	theme	major	1694:1698	arg1	minerals					1726:1733	minerals	1726:1733	minerals	1726:1733	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	0	58	theme	Aloe	48:51	arg1	vera					53:56	Aloe vera	48:56	Aloe vera	48:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	14	59	theme	A.	2546:2547	arg1	leaf					2554:2557	A. vera leaf	2546:2557	A. vera leaf	2546:2557	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	15	60	theme	SEC	2609:2611	arg1	tool					2691:2694	an improved tool	2679:2694	an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study	2679:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	60	theme	SEC	2609:2611	arg1	use					2598:2600	The use	2594:2600	The use of the SEC combined with MALS and differential RI detectors	2594:2660	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	1	61	theme	chemical	207:214	arg1	composition					216:226	the chemical composition	203:226	the chemical composition of the Aloe vera plant	203:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	14	62	theme	composition	2531:2541	arg1	investigation					2495:2507	a comprehensive investigation	2479:2507	a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products	2479:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	1	63	theme	substantial	132:142	arg1	number					144:149	a substantial number	130:149	a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant	130:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	15	64	from	study	2824:2828	arg1	contents					2752:2759	contents	2752:2759	contents	2752:2759	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	64	from	study	2824:2828	arg1	MW					2745:2746	polysaccharide MW	2730:2746	polysaccharide MW	2730:2746	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	11	65	theme	fiber	1931:1935	arg1	fiber					1931:1935	fiber	1931:1935	fiber	1931:1935	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	65	theme	fiber	1931:1935	arg1	amounts					1920:1926	high amounts	1915:1926	high amounts of fiber and protein	1915:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	65	theme	fiber	1931:1935	arg1	protein					1941:1947	protein	1941:1947	protein	1941:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	2	66	dep	fresh	392:396	arg1	leaves					398:403	leaves	398:403	leaves	398:403	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	14	67	theme	major	2516:2520	arg1	composition					2531:2541	the major chemical composition	2512:2541	the major chemical composition of A. vera leaf and commercially derived products	2512:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	9	68	theme	composition	1618:1628	arg1	%					1603:1603	85-95%	1598:1603	85-95% of the total composition determined	1598:1639	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	9	68	theme	composition	1618:1628	arg1	composition					1618:1628	the total composition	1608:1628	the total composition determined	1608:1639	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	7	69	theme	light	1274:1278	arg1	MALS					1292:1295	MALS	1292:1295	MALS	1292:1295	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	69	theme	light	1274:1278	arg1	scattering					1280:1289	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	69	theme	light	1274:1278	arg1	minerals					1074:1081	minerals	1074:1081	minerals	1074:1081	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	70	theme	exclusion	1225:1233	arg1	chromatography					1235:1248	size exclusion chromatography	1220:1248	size exclusion chromatography	1220:1248	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	71	theme	pulsed	1145:1150	arg1	detection					1165:1173	pulsed amperometric detection	1145:1173	pulsed amperometric detection for free sugars	1145:1189	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	1	72	theme	Aloe	235:238	arg1	plant					245:249	the Aloe vera plant	231:249	the Aloe vera plant	231:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	7	73	theme	-multi-angle	1255:1266	arg1	MALS					1292:1295	MALS	1292:1295	MALS	1292:1295	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	73	theme	-multi-angle	1255:1266	arg1	scattering					1280:1289	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	73	theme	-multi-angle	1255:1266	arg1	minerals					1074:1081	minerals	1074:1081	minerals	1074:1081	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	4	74	theme	A.	561:562	arg1	vera					564:567	Fresh A. vera	555:567	Fresh A. vera	555:567	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	2	75	theme	investigations	266:279	arg1	None					252:255	None	252:255	None of these investigations	252:279	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	1	76	theme	plant	245:249	arg1	composition					216:226	the chemical composition	203:226	the chemical composition of the Aloe vera plant	203:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	14	77	theme	comprehensive	2481:2493	arg1	investigation					2495:2507	a comprehensive investigation	2479:2507	a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products	2479:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	7	78	theme	anion-exchange	1101:1114	arg1	chromatography					1116:1129	high-performance anion-exchange chromatography	1084:1129	high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars	1084:1189	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	78	theme	anion-exchange	1101:1114	arg1	minerals					1074:1081	minerals	1074:1081	minerals	1074:1081	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	1	79	theme	papers	154:159	arg1	number					144:149	a substantial number	130:149	a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant	130:249	Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant.					
29954478	13	80	theme	aloe	2336:2339	arg1	samples					2341:2347	the commercial aloe samples	2321:2347	the commercial aloe samples analyzed	2321:2356	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	15	81	theme	RI	2649:2650	arg1	detectors					2652:2660	differential RI detectors	2636:2660	differential RI detectors	2636:2660	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	82	theme	various	2768:2774	arg1	products					2807:2814	the various commercially available A. vera products	2764:2814	the various commercially available A. vera products in this study	2764:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	5	83	theme	manufacturing	824:836	arg1	processes					838:846	manufacturing processes	824:846	manufacturing processes	824:846	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	4	84	theme	aloe	603:606	arg1	powders					614:620	commercial aloe juice powders	592:620	commercial aloe juice powders	592:620	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	7	85	theme	emission	1048:1055	arg1	spectroscopy					1057:1068	inductively coupled plasma-optical emission spectroscopy	1013:1068	inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1013:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	10	86	theme	composition	1807:1817	arg1	composition					1807:1817	the total composition	1797:1817	the total composition	1797:1817	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	86	theme	composition	1807:1817	arg1	%					1792:1792	78-84%	1787:1792	78-84% of the total composition	1787:1817	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	9	87	theme	fresh	1482:1486	arg1	leaf					1488:1491	A. vera fresh leaf	1474:1491	A. vera fresh leaf	1474:1491	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	6	88	theme	organic	923:929	arg1	acids					931:935	organic acids	923:935	organic acids	923:935	The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides.					
29954478	6	88	theme	organic	923:929	arg1	lipids					905:910	lipids	905:910	lipids	905:910	The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides.					
29954478	4	89	theme	powders	614:620	arg1	number					582:587	a number	580:587	a number of commercial aloe juice powders	580:620	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	7	90	theme	coupled	1025:1031	arg1	spectroscopy					1057:1068	inductively coupled plasma-optical emission spectroscopy	1013:1068	inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1013:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	13	91	theme	commercial	2325:2334	arg1	samples					2341:2347	the commercial aloe samples	2321:2347	the commercial aloe samples analyzed	2321:2356	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	7	92	theme	analytical	976:985	arg1	methods					987:993	The analytical methods	972:993	The analytical methods employed	972:1002	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	9	93	theme	A.	1474:1475	arg1	vera					1477:1480	A. vera	1474:1480	A. vera fresh leaf	1474:1491	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	4	94	theme	chemical	656:663	arg1	constituents					665:676	their major chemical constituents	644:676	their major chemical constituents	644:676	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	7	95	theme	size	1220:1223	arg1	chromatography					1235:1248	size exclusion chromatography	1220:1248	size exclusion chromatography	1220:1248	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	0	96	theme	Commercial	77:86	arg1	Powders					99:105	and Several Commercial Aloe Juice Powders	65:105	Powders	99:105	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	15	97	from	MW	2745:2746	arg1	study					2824:2828	this study	2819:2828	this study	2819:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	5	98	dep	fresh	696:700	arg1	leaves					702:707	leaves	702:707	leaves from China and Mexico	702:729	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	0	99	from	Investigation	9:21	arg1	vera					53:56	Aloe vera	48:56	Aloe vera	48:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	0	100	theme	Juice	93:97	arg1	Powders					99:105	and Several Commercial Aloe Juice Powders	65:105	Powders	99:105	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	13	101	theme	polysaccharides	2302:2316	arg1	content					2291:2297	content	2291:2297	content	2291:2297	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	13	101	theme	polysaccharides	2302:2316	arg1	MW					2284:2285	MW	2284:2285	MW	2284:2285	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	14	102	theme	products	2584:2591	arg1	composition					2531:2541	the major chemical composition	2512:2541	the major chemical composition of A. vera leaf and commercially derived products	2512:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	0	103	from	vera	53:56	arg1	Investigation					9:21	Chemical Investigation	0:21	Chemical Investigation of Major Constituents in Aloe vera	0:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	11	104	theme	major	1840:1844	arg1	components					1846:1855	the four major components	1831:1855	the four major components	1831:1855	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	105	contain	contained	1905:1913	arg2	protein					1941:1947	protein	1941:1947	protein	1941:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	105	contain	contained	1905:1913	arg2	fiber					1931:1935	fiber	1931:1935	fiber	1931:1935	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	105	contain	contained	1905:1913	arg2	amounts					1920:1926	high amounts	1915:1926	high amounts of fiber and protein	1915:1947	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	105	contain	contained	1905:1913	arg1	products					1858:1865	products	1858:1865	products manufactured by ethanol precipitation	1858:1903	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	15	106	theme	contents	2752:2759	arg1	determination					2713:2725	the accurate determination	2700:2725	the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study	2700:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	15	107	from	contents	2752:2759	arg1	study					2824:2828	this study	2819:2828	this study	2819:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	5	108	theme	different	795:803	arg1	parts					811:815	different plant parts	795:815	different plant parts	795:815	Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes.					
29954478	13	109	theme	manufacturing	2404:2416	arg1	processes					2418:2426	manufacturing processes	2404:2426	manufacturing processes employed	2404:2435	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	14	110	theme	chemical	2522:2529	arg1	composition					2531:2541	the major chemical composition	2512:2541	the major chemical composition of A. vera leaf and commercially derived products	2512:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	2	111	theme	processing	367:376	arg1	variations					378:387	processing variations	367:387	processing variations in fresh leaves and commercially available product forms	367:444	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	0	112	theme	Major	26:30	arg1	Constituents					32:43	Major Constituents	26:43	Major Constituents in Aloe vera	26:56	Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders.					
29954478	12	113	theme	nonethanol	2174:2183	arg1	processes					2199:2207	the nonethanol manufacturing processes	2170:2207	the nonethanol manufacturing processes	2170:2207	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	2	114	theme	product	432:438	arg1	forms					440:444	commercially available product forms	409:444	commercially available product forms	409:444	None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms.					
29954478	4	115	theme	Fresh	555:559	arg1	vera					564:567	Fresh A. vera	555:567	Fresh A. vera	555:567	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	13	116	dep	MW	2284:2285	arg1	the					2280:2282	the	2280:2282	the	2280:2282	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	8	117	theme	MW	1383:1384	arg1	distribution					1386:1397	MW distribution	1383:1397	MW distribution	1383:1397	The absolute MW and MW distribution were determined using MALS measurement.					
29954478	14	118	link	derived	2576:2582	arg1	products					2584:2591	commercially derived products	2563:2591	commercially derived products	2563:2591	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	10	119	theme	aloe	1669:1672	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	119	theme	aloe	1669:1672	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	7	120	theme	free	1179:1182	arg1	sugars					1184:1189	free sugars	1179:1189	free sugars	1179:1189	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	121	theme	-differential	1297:1309	arg1	index					1322:1326	-differential refractive index	1297:1326	minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses	1074:1360	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	121	theme	-differential	1297:1309	arg1	dRI					1329:1331	dRI	1329:1331	dRI	1329:1331	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	12	122	theme	manufacturing	2185:2197	arg1	processes					2199:2207	the nonethanol manufacturing processes	2170:2207	the nonethanol manufacturing processes	2170:2207	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	14	123	theme	present	2456:2462	arg1	study					2464:2468	This present study	2451:2468	This present study	2451:2468	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	10	124	theme	commercial	1649:1658	arg1	acids					1719:1723	four major components-organic acids	1689:1723	four major components-organic acids	1689:1723	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	10	124	theme	commercial	1649:1658	arg1	samples					1680:1686	the commercial powdered aloe juice samples	1645:1686	the commercial powdered aloe juice samples	1645:1686	In the commercial powdered aloe juice samples, four major components-organic acids, minerals, monosaccharides, and polysaccharides-accounted for 78-84% of the total composition.					
29954478	15	125	theme	accurate	2704:2711	arg1	determination					2713:2725	the accurate determination	2700:2725	the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study	2700:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	13	126	theme	chemical	2222:2229	arg1	profiles					2231:2238	The overall chemical profiles	2210:2238	The overall chemical profiles	2210:2238	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	13	126	theme	chemical	2222:2229	arg1	consistent					2257:2266	consistent	2257:2266	consistent	2257:2266	The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed.					
29954478	14	127	theme	vera	2549:2552	arg1	leaf					2554:2557	A. vera leaf	2546:2557	A. vera leaf	2546:2557	Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products.					
29954478	6	128	theme	test	853:856	arg1	results					858:864	The test results	849:864	The test results	849:864	The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides.					
29954478	15	129	from	products	2807:2814	arg1	study					2824:2828	this study	2819:2828	this study	2819:2828	The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.					
29954478	11	130	theme	free	1960:1963	arg1	sugars					1965:1970	the free sugars	1956:1970	the free sugars	1956:1970	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	11	131	theme	ethanol	1883:1889	arg1	precipitation					1891:1903	ethanol precipitation	1883:1903	ethanol precipitation	1883:1903	Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed.					
29954478	12	132	theme	polysaccharide	2024:2037	arg1	MW					2039:2040	the polysaccharide MW	2020:2040	the polysaccharide MW	2020:2040	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	12	133	theme	polysaccharides	2118:2132	arg1	higher					2138:2143	higher	2138:2143	higher	2138:2143	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	12	133	theme	polysaccharides	2118:2132	arg1	concentration					2096:2108	the concentration	2092:2108	the concentration of aloe polysaccharides	2092:2132	In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes.					
29954478	9	134	theme	total	1612:1616	arg1	composition					1618:1628	the total composition	1608:1628	the total composition determined	1608:1639	Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85-95% of the total composition determined.					
29954478	7	135	theme	laser	1268:1272	arg1	MALS					1292:1295	MALS	1292:1295	MALS	1292:1295	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	135	theme	laser	1268:1272	arg1	scattering					1280:1289	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)	1220:1296	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	7	135	theme	laser	1268:1272	arg1	minerals					1074:1081	minerals	1074:1081	minerals	1074:1081	The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)-multi-angle laser light scattering (MALS)-differential refractive index (dRI) for polysaccharide analyses.					
29954478	4	136	theme	commercial	592:601	arg1	powders					614:620	commercial aloe juice powders	592:620	commercial aloe juice powders	592:620	Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents.					
29954478	3	137	theme	forms	514:518	arg1	range					499:503	a range	497:503	a range of these forms	497:518	Objectives: This work was to analytically examine a range of these forms and compile the findings.					
31591262	2	0	from	study	329:333	arg1	glycosylation					342:354	the glycosylation	338:354	the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	338:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	10	1	with	Agreement	1507:1515	arg1	medians					1536:1542	the consensus medians	1522:1542	the consensus medians	1522:1542	Agreement with the consensus medians did not depend on the specific method or laboratory type.					
31591262	0	2	theme	NIST	0:3	arg1	Study					21:25	NIST Interlaboratory Study	0:25	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.	0:132	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	1	3	theme	interest	178:185	arg1	Glycosylation					134:146	Glycosylation	134:146	Glycosylation	134:146	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	1	3	theme	interest	178:185	arg1	topic					153:157	a topic	151:157	a topic of intense current interest in the development of biopharmaceuticals	151:226	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	4	4	theme	principal	640:648	arg1	objective					650:658	The principal objective	636:658	The principal objective of this study	636:672	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	11	5	theme	analysis	1760:1767	arg1	methods					1769:1775	glycosylation analysis methods	1746:1775	glycosylation analysis methods	1746:1775	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	5	6	theme	measurement	883:893	arg1	techniques					895:904	their own measurement techniques	873:904	their own measurement techniques	873:904	Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques.					
31591262	0	7	from	Comparison	79:88	arg1	Methods					125:131	Diverse Analytical Methods	106:131	Diverse Analytical Methods	106:131	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	10	8	theme	specific	1566:1573	arg1	method					1575:1580	the specific method	1562:1580	the specific method	1562:1580	Agreement with the consensus medians did not depend on the specific method or laboratory type.					
31591262	9	9	theme	consensus	1440:1448	arg1	medians					1450:1456	These consensus medians	1434:1456	These consensus medians	1434:1456	These consensus medians provide community-derived values for NISTmAb PS.					
31591262	6	10	theme	Protein	907:913	arg1	glycosylation					915:927	Protein glycosylation	907:927	Protein glycosylation	907:927	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	8	11	theme	consensus	1406:1414	arg1	values					1426:1431	consensus abundance values	1406:1431	consensus abundance values	1406:1431	In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values.					
31591262	11	12	theme	clear	1714:1718	arg1	need					1720:1723	a clear need	1712:1723	a clear need for harmonization of glycosylation analysis methods	1712:1775	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	6	13	theme	mAb	994:996	arg1	level					978:982	the level	974:982	the level of intact mAb, protein fragments, glycopeptides, or released glycans	974:1051	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	0	14	theme	Antibodies	67:76	arg1	Analysis					44:51	Glycosylation Analysis	30:51	Glycosylation Analysis of Monoclonal Antibodies	30:76	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	0	15	from	Study	21:25	arg1	Analysis					44:51	Glycosylation Analysis	30:51	Glycosylation Analysis of Monoclonal Antibodies	30:76	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	4	16	theme	study	668:672	arg1	objective					650:658	The principal objective	636:658	The principal objective of this study	636:672	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	3	17	from	sectors	524:530	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	1	18	theme	intense	162:168	arg1	interest					178:185	intense current interest	162:185	intense current interest	162:185	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	0	19	theme	Results	93:99	arg1	Comparison					79:88	Comparison	79:88	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.	0:132	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	4	20	theme	analytical	730:739	arg1	methods					741:747	analytical methods	730:747	analytical methods presently used in the glycosylation analysis of mAbs	730:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	0	21	theme	Diverse	106:112	arg1	Methods					125:131	Diverse Analytical Methods	106:131	Diverse Analytical Methods	106:131	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	6	22	theme	released	1036:1043	arg1	glycans					1045:1051	released glycans	1036:1051	released glycans	1036:1051	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	4	23	gly	glycosylation	771:783	arg1	mAbs					797:800	mAbs	797:800	mAbs	797:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	2	24	theme	reference	417:425	arg1	material					427:434	a monoclonal antibody reference material	395:434	a monoclonal antibody reference material	395:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	24	theme	reference	417:425	arg1	NISTmAb					386:392	NISTmAb	386:392	NISTmAb	386:392	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	25	theme	monoclonal	397:406	arg1	antibody					408:415	a monoclonal antibody	395:415	a monoclonal antibody reference material	395:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	8	26	dep	reported	1353:1360	arg1	assigned					1397:1404	assigned	1397:1404	could be assigned consensus abundance values	1388:1431	In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values.					
31591262	3	27	theme	glycan	614:619	arg1	distributions					621:633	glycan distributions	614:633	glycan distributions	614:633	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	4	28	theme	glycosylation	771:783	arg1	analysis					785:792	the glycosylation analysis	767:792	the glycosylation analysis of mAbs	767:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	3	29	theme	Seventy-six	437:447	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	0	30	theme	Glycosylation	30:42	arg1	Analysis					44:51	Glycosylation Analysis	30:51	Glycosylation Analysis of Monoclonal Antibodies	30:76	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	6	31	theme	protein	999:1005	arg1	fragments					1007:1015	protein fragments	999:1015	protein fragments	999:1015	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	3	32	theme	reports	603:609	arg1	total					590:594	a total	588:594	a total of 103 reports	588:609	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	3	33	from	laboratories	449:460	arg1	North					543:547	North	543:547	North	543:547	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	3	33	from	laboratories	449:460	arg1	Asia					558:561	Asia	558:561	Asia	558:561	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	3	33	from	laboratories	449:460	arg1	Australia					568:576	Australia	568:576	Australia	568:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	3	33	from	laboratories	449:460	arg1	America					549:555	America	549:555	America	549:555	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	6	34	theme	methods	1078:1084	arg1	separation					1106:1115	separation	1106:1115	separation	1106:1115	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	34	theme	methods	1078:1084	arg1	methods					1078:1084	methods	1078:1084	methods for derivatization	1078:1103	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	34	theme	methods	1078:1084	arg1	identification					1118:1131	identification	1118:1131	identification	1118:1131	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	34	theme	methods	1078:1084	arg1	variety					1067:1073	a wide variety	1060:1073	a wide variety of methods for derivatization, separation, identification, and quantification	1060:1151	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	34	theme	methods	1078:1084	arg1	quantification					1138:1151	quantification	1138:1151	quantification	1138:1151	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	3	35	from	industry	467:474	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	0	36	dep	Study	21:25	arg1	Comparison					79:88	Comparison	79:88	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.	0:132	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	11	37	theme	glycosylation	1673:1685	arg1	measurement					1687:1697	biologic glycosylation measurement	1664:1697	biologic glycosylation measurement	1664:1697	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	2	38	theme	Sample	371:376	arg1	glycosylation					342:354	the glycosylation	338:354	the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	338:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	7	39	theme	compositions	1233:1244	arg1	number					1216:1221	the number	1212:1221	the number of glycan compositions identified by each laboratory ranging from 4 to 48	1212:1295	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	0	40	from	Methods	125:131	arg1	Comparison					79:88	Comparison	79:88	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.	0:132	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	0	40	from	Methods	125:131	arg1	Results					93:99	Results	93:99	Results from Diverse Analytical Methods	93:131	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	7	41	with	enormous	1197:1204	arg1	number					1216:1221	the number	1212:1221	the number of glycan compositions identified by each laboratory ranging from 4 to 48	1212:1295	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	11	42	theme	current	1635:1641	arg1	state-of-the-art					1643:1658	the current state-of-the-art	1631:1658	the current state-of-the-art for biologic glycosylation measurement	1631:1697	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	3	43	from	government	499:508	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	2	44	from	glycosylation	342:354	arg1	results					299:305	results	299:305	results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	299:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	10	45	theme	consensus	1526:1534	arg1	medians					1536:1542	the consensus medians	1522:1542	the consensus medians	1522:1542	Agreement with the consensus medians did not depend on the specific method or laboratory type.					
31591262	6	46	gly	glycosylation	915:927	arg1	ways					955:958	various ways	947:958	various ways	947:958	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	3	47	theme	hospital	515:522	arg1	sectors					524:530	hospital sectors	515:530	hospital sectors	515:530	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	1	48	theme	current	170:176	arg1	interest					178:185	intense current interest	162:185	intense current interest	162:185	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	4	49	theme	mAbs	797:800	arg1	analysis					785:792	the glycosylation analysis	767:792	the glycosylation analysis of mAbs	767:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	3	50	from	university	477:486	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	2	51	theme	interlaboratory	313:327	arg1	study					329:333	an interlaboratory study	310:333	an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	310:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	3	52	from	research	489:496	arg1	laboratories					449:460	Seventy-six laboratories	437:460	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia	437:576	Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions.					
31591262	7	53	dep	48	1294:1295	arg1	to					1291:1292	to	1291:1292	to	1291:1292	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	6	54	theme	various	947:953	arg1	ways					955:958	various ways	947:958	various ways	947:958	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	9	55	theme	NISTmAb	1495:1501	arg1	PS					1503:1504	NISTmAb PS	1495:1504	NISTmAb PS	1495:1504	These consensus medians provide community-derived values for NISTmAb PS.					
31591262	10	56	theme	laboratory	1585:1594	arg1	type					1596:1599	laboratory type	1585:1599	laboratory type	1585:1599	Agreement with the consensus medians did not depend on the specific method or laboratory type.					
31591262	11	57	theme	methods	1769:1775	arg1	harmonization					1729:1741	harmonization	1729:1741	harmonization of glycosylation analysis methods	1729:1775	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	1	58	theme	biopharmaceuticals	209:226	arg1	development					194:204	the development	190:204	the development of biopharmaceuticals	190:226	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	5	59	theme	own	879:881	arg1	techniques					895:904	their own measurement techniques	873:904	their own measurement techniques	873:904	Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques.					
31591262	8	60	theme	abundance	1416:1424	arg1	values					1426:1431	consensus abundance values	1406:1431	consensus abundance values	1406:1431	In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values.					
31591262	1	61	dep	drug	253:256	arg1	safety					258:263	safety	258:263	safety	258:263	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	1	61	dep	drug	253:256	arg1	efficacy					269:276	efficacy	269:276	efficacy	269:276	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	7	62	theme	results	1185:1191	arg1	enormous					1197:1204	enormous	1197:1204	enormous	1197:1204	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	7	62	theme	results	1185:1191	arg1	diversity					1172:1180	the diversity	1168:1180	the diversity of results	1168:1191	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	9	63	theme	community-derived	1466:1482	arg1	values					1484:1489	community-derived values	1466:1489	community-derived values for NISTmAb PS	1466:1504	These consensus medians provide community-derived values for NISTmAb PS.					
31591262	6	64	theme	intact	987:992	arg1	mAb					994:996	intact mAb	987:996	intact mAb	987:996	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	2	65	from	results	299:305	arg1	glycosylation					342:354	the glycosylation	338:354	the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	338:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	11	66	theme	glycosylation	1746:1758	arg1	methods					1769:1775	glycosylation analysis methods	1746:1775	glycosylation analysis methods	1746:1775	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	4	67	theme	full	716:719	arg1	range					721:725	the full range	712:725	the full range of analytical methods presently used in the glycosylation analysis of mAbs	712:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	0	68	theme	Analytical	114:123	arg1	Methods					125:131	Diverse Analytical Methods	106:131	Diverse Analytical Methods	106:131	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	1	69	from	topic	153:157	arg1	development					194:204	the development	190:204	the development of biopharmaceuticals	190:226	Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy.					
31591262	6	70	theme	glycans	1045:1051	arg1	level					978:982	the level	974:982	the level of intact mAb, protein fragments, glycopeptides, or released glycans	974:1051	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	0	71	theme	Interlaboratory	5:19	arg1	Study					21:25	NIST Interlaboratory Study	0:25	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.	0:132	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	6	72	theme	glycopeptides	1018:1030	arg1	level					978:982	the level	974:982	the level of intact mAb, protein fragments, glycopeptides, or released glycans	974:1051	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	2	73	theme	antibody	408:415	arg1	material					427:434	a monoclonal antibody reference material	395:434	a monoclonal antibody reference material	395:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	73	theme	antibody	408:415	arg1	NISTmAb					386:392	NISTmAb	386:392	NISTmAb	386:392	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	6	74	gly	glycopeptides	1018:1030	arg2	glycopeptides					1018:1030	glycopeptides	1018:1030	glycopeptides	1018:1030	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	11	75	theme	state-of-the-art	1643:1658	arg1	view					1623:1626	a view	1621:1626	a view of the current state-of-the-art for biologic glycosylation measurement	1621:1697	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	9	76	link	community-derived	1466:1482	arg1	values					1484:1489	community-derived values	1466:1489	community-derived values for NISTmAb PS	1466:1504	These consensus medians provide community-derived values for NISTmAb PS.					
31591262	6	77	theme	fragments	1007:1015	arg1	level					978:982	the level	974:982	the level of intact mAb, protein fragments, glycopeptides, or released glycans	974:1051	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	8	78	theme	glycan	1328:1333	arg1	compositions					1335:1346	one hundred sixteen glycan compositions	1308:1346	one hundred sixteen glycan compositions	1308:1346	In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values.					
31591262	2	79	theme	NISTmAb	386:392	arg1	PS					379:380	PS	379:380	PS	379:380	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	79	theme	NISTmAb	386:392	arg1	Sample					371:376	the Primary Sample	359:376	the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	359:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	0	80	theme	Monoclonal	56:65	arg1	Antibodies					67:76	Monoclonal Antibodies	56:76	Monoclonal Antibodies	56:76	NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.					
31591262	7	81	theme	glycan	1226:1231	arg1	compositions					1233:1244	glycan compositions	1226:1244	glycan compositions identified by each laboratory ranging from 4 to 48	1226:1295	Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48.					
31591262	2	82	gly	glycosylation	342:354	arg1	PS					379:380	PS	379:380	PS	379:380	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	82	gly	glycosylation	342:354	arg1	Sample					371:376	the Primary Sample	359:376	the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	359:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	11	83	theme	biologic	1664:1671	arg1	measurement					1687:1697	biologic glycosylation measurement	1664:1697	biologic glycosylation measurement	1664:1697	The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.					
31591262	4	84	theme	methods	741:747	arg1	range					721:725	the full range	712:725	the full range of analytical methods presently used in the glycosylation analysis of mAbs	712:800	The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs.					
31591262	2	85	theme	study	329:333	arg1	results					299:305	results	299:305	results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	299:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	6	86	theme	wide	1062:1065	arg1	separation					1106:1115	separation	1106:1115	separation	1106:1115	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	86	theme	wide	1062:1065	arg1	methods					1078:1084	methods	1078:1084	methods for derivatization	1078:1103	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	86	theme	wide	1062:1065	arg1	identification					1118:1131	identification	1118:1131	identification	1118:1131	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	86	theme	wide	1062:1065	arg1	variety					1067:1073	a wide variety	1060:1073	a wide variety of methods for derivatization, separation, identification, and quantification	1060:1151	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	6	86	theme	wide	1062:1065	arg1	quantification					1138:1151	quantification	1138:1151	quantification	1138:1151	Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification.					
31591262	2	87	theme	Primary	363:369	arg1	PS					379:380	PS	379:380	PS	379:380	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
31591262	2	87	theme	Primary	363:369	arg1	Sample					371:376	the Primary Sample	359:376	the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material	359:434	This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material.					
30904529	0	0	theme	chondroitin	100:110	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	2	1	from	floridana	495:503	arg1	FCS					475:477	FCS	475:477	FCS	475:477	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	2	1	from	floridana	495:503	arg1	fucoidan					462:469	fucoidan	462:469	fucoidan	462:469	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	0	2	theme	fucosylated	88:98	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	3	3	theme	fucoidan	742:749	arg1	depolymerization					722:737	the hydrothermal depolymerization	705:737	the hydrothermal depolymerization of fucoidan and FCS	705:757	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	3	4	theme	key	693:695	arg1	role					697:700	a key role	691:700	a key role	691:700	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	1	5	theme	Holothuria	289:298	arg1	floridana					300:308	Holothuria floridana	289:308	Holothuria floridana	289:308	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	6	6	theme	different	1375:1383	arg1	structure					1385:1393	their different structure	1369:1393	their different structure	1369:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	4	7	theme	FCS	898:900	arg1	structure					831:839	the structure	827:839	the structure of the optimized hydrothermal depolymerized fucoidan and FCS	827:900	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	5	8	theme	hydrothermal	1016:1027	arg1	condition					1029:1037	the same hydrothermal condition	1007:1037	the same hydrothermal condition	1007:1037	By comparison, FCS was more difficult to be depolymerized than fucoidan under the same hydrothermal condition.					
30904529	7	9	from	mechanism	1466:1474	arg1	cucumber					1505:1512	sea cucumber	1501:1512	sea cucumber	1501:1512	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	6	10	theme	glycosidic	1205:1214	arg1	linkage					1216:1222	β-1 → 4 glycosidic linkage	1197:1222	β-1 → 4 glycosidic linkage	1197:1222	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	11	theme	decarboxylation	1228:1242	arg1	cleavage					1185:1192	preferential cleavage	1172:1192	preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure	1172:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	7	12	theme	FCS	1492:1494	arg1	depolymerization					1438:1453	hydrothermal depolymerization	1425:1453	hydrothermal depolymerization	1425:1453	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	12	theme	FCS	1492:1494	arg1	mechanism					1466:1474	action mechanism	1459:1474	action mechanism	1459:1474	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	0	13	gly	fucosylated	88:98	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	2	14	theme	pH	593:594	arg1	control					582:588	control	582:588	control of pH at 5-6	582:601	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	6	15	theme	due	1362:1364	arg1	type					1345:1348	the random fracture type	1325:1348	the random fracture type of fucoidan due to their different structure	1325:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	16	theme	hydrothermal	1270:1281	arg1	treatment					1283:1291	hydrothermal treatment	1270:1291	hydrothermal treatment	1270:1291	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	17	theme	preferential	1172:1183	arg1	cleavage					1185:1192	preferential cleavage	1172:1192	preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure	1172:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	7	18	theme	action	1459:1464	arg1	mechanism					1466:1474	action mechanism	1459:1474	action mechanism	1459:1474	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	6	19	theme	fucoidan	1353:1360	arg1	type					1345:1348	the random fracture type	1325:1348	the random fracture type of fucoidan due to their different structure	1325:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	7	20	theme	hydrothermal	1425:1436	arg1	depolymerization					1438:1453	hydrothermal depolymerization	1425:1453	hydrothermal depolymerization	1425:1453	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	6	21	theme	oligosaccharide	1044:1058	arg1	profile					1060:1066	The oligosaccharide profile	1040:1066	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis	1040:1121	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	1	22	gly	fucosylated	246:256	arg1	FCS					279:281	FCS	279:281	FCS	279:281	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	1	22	gly	fucosylated	246:256	arg1	sulfate					270:276	fucosylated chondroitin sulfate	246:276	fucosylated chondroitin sulfate (FCS)	246:282	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	0	23	theme	depolymerization	27:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan	0:82	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	0	23	theme	depolymerization	27:42	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	4	24	theme	hydrothermal	858:869	arg1	fucoidan					885:892	the optimized hydrothermal depolymerized fucoidan	844:892	the optimized hydrothermal depolymerized fucoidan	844:892	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	1	25	from	floridana	300:308	arg1	FCS					279:281	FCS	279:281	FCS	279:281	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	1	25	from	floridana	300:308	arg1	sulfate					270:276	fucosylated chondroitin sulfate	246:276	fucosylated chondroitin sulfate (FCS)	246:282	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	1	25	from	floridana	300:308	arg1	fucoidan					233:240	fucoidan	233:240	fucoidan	233:240	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	5	26	theme	same	1011:1014	arg1	condition					1029:1037	the same hydrothermal condition	1007:1037	the same hydrothermal condition	1007:1037	By comparison, FCS was more difficult to be depolymerized than fucoidan under the same hydrothermal condition.					
30904529	7	27	theme	sea	1501:1503	arg1	cucumber					1505:1512	sea cucumber	1501:1512	sea cucumber	1501:1512	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	28	from	cucumber	1505:1512	arg1	fucoidan					1479:1486	fucoidan	1479:1486	fucoidan	1479:1486	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	28	from	cucumber	1505:1512	arg1	depolymerization					1438:1453	hydrothermal depolymerization	1425:1453	hydrothermal depolymerization	1425:1453	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	28	from	cucumber	1505:1512	arg1	mechanism					1466:1474	action mechanism	1459:1474	action mechanism	1459:1474	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	28	from	cucumber	1505:1512	arg1	FCS					1492:1494	FCS	1492:1494	FCS	1492:1494	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	0	29	theme	hydrothermal	14:25	arg1	depolymerization					27:42	hydrothermal depolymerization	14:42	hydrothermal depolymerization	14:42	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	1	30	theme	hydrothermal	335:346	arg1	conditions					348:357	hydrothermal conditions	335:357	hydrothermal conditions	335:357	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	2	31	theme	molecular	624:632	arg1	weight					634:639	controlled molecular weight	613:639	controlled molecular weight	613:639	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	4	32	theme	NMR	802:804	arg1	analysis					806:813	NMR analysis	802:813	NMR analysis	802:813	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	6	33	theme	β-1 → 4	1197:1203	arg1	linkage					1216:1222	β-1 → 4 glycosidic linkage	1197:1222	β-1 → 4 glycosidic linkage	1197:1222	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	0	34	theme	oligosaccharide	48:62	arg1	profile					64:70	oligosaccharide profile	48:70	oligosaccharide profile	48:70	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	3	35	theme	hydrothermal	709:720	arg1	depolymerization					722:737	the hydrothermal depolymerization	705:737	the hydrothermal depolymerization of fucoidan and FCS	705:757	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	2	36	theme	controlled	613:622	arg1	weight					634:639	controlled molecular weight	613:639	controlled molecular weight	613:639	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	4	37	theme	fucoidan	885:892	arg1	structure					831:839	the structure	827:839	the structure of the optimized hydrothermal depolymerized fucoidan and FCS	827:900	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	6	38	from	acid	1258:1261	arg1	cleavage					1185:1192	preferential cleavage	1172:1192	preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure	1172:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	1	39	theme	fucosylated	246:256	arg1	FCS					279:281	FCS	279:281	FCS	279:281	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	1	39	theme	fucosylated	246:256	arg1	sulfate					270:276	fucosylated chondroitin sulfate	246:276	fucosylated chondroitin sulfate (FCS)	246:282	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	6	40	theme	depolymerized	1071:1083	arg1	fucoidan					1085:1092	depolymerized fucoidan	1071:1092	depolymerized fucoidan	1071:1092	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	4	41	theme	depolymerized	871:883	arg1	fucoidan					885:892	the optimized hydrothermal depolymerized fucoidan	844:892	the optimized hydrothermal depolymerized fucoidan	844:892	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	6	42	from	linkage	1216:1222	arg1	acid					1258:1261	glucuronic acid	1247:1261	glucuronic acid	1247:1261	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	1	43	theme	chondroitin	258:268	arg1	FCS					279:281	FCS	279:281	FCS	279:281	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	1	43	theme	chondroitin	258:268	arg1	sulfate					270:276	fucosylated chondroitin sulfate	246:276	fucosylated chondroitin sulfate (FCS)	246:282	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	2	44	from	control	582:588	arg1	5-6					599:601	5-6	599:601	5-6	599:601	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	3	45	theme	first	653:657	arg1	It					642:643	It	642:643	It	642:643	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	3	45	theme	first	653:657	arg1	time					659:662	the first time	649:662	the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS	649:757	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	4	46	theme	monosaccharide	764:777	arg1	composition					779:789	The monosaccharide composition	760:789	The monosaccharide composition	760:789	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30904529	0	47	theme	profile	64:70	arg1	Comparison					0:9	Comparison	0:9	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan	0:82	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	0	47	theme	profile	64:70	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	3	48	theme	FCS	755:757	arg1	depolymerization					722:737	the hydrothermal depolymerization	705:737	the hydrothermal depolymerization of fucoidan and FCS	705:757	It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS.					
30904529	0	49	from	floridana	136:144	arg1	Comparison					0:9	Comparison	0:9	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan	0:82	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	0	49	from	floridana	136:144	arg1	sulfate					112:118	fucosylated chondroitin sulfate	88:118	fucosylated chondroitin sulfate from Holothuria floridana	88:144	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	6	50	from	profile	1060:1066	arg1	FCS					1098:1100	FCS	1098:1100	FCS	1098:1100	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	50	from	profile	1060:1066	arg1	fucoidan					1085:1092	depolymerized fucoidan	1071:1092	depolymerized fucoidan	1071:1092	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	51	from	decarboxylation	1228:1242	arg1	acid					1258:1261	glucuronic acid	1247:1261	glucuronic acid	1247:1261	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	52	with	different	1310:1318	arg1	type					1345:1348	the random fracture type	1325:1348	the random fracture type of fucoidan due to their different structure	1325:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	7	53	theme	fucoidan	1479:1486	arg1	depolymerization					1438:1453	hydrothermal depolymerization	1425:1453	hydrothermal depolymerization	1425:1453	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	7	53	theme	fucoidan	1479:1486	arg1	mechanism					1466:1474	action mechanism	1459:1474	action mechanism	1459:1474	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	6	54	theme	fracture	1336:1343	arg1	type					1345:1348	the random fracture type	1325:1348	the random fracture type of fucoidan due to their different structure	1325:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	55	theme	linkage	1216:1222	arg1	cleavage					1185:1192	preferential cleavage	1172:1192	preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure	1172:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	56	theme	glucuronic	1247:1256	arg1	acid					1258:1261	glucuronic acid	1247:1261	glucuronic acid	1247:1261	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	57	theme	HILIC-MS	1105:1112	arg1	analysis					1114:1121	HILIC-MS analysis	1105:1121	HILIC-MS analysis	1105:1121	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	7	58	theme	different	1545:1553	arg1	structure					1555:1563	their different structure	1539:1563	their different structure	1539:1563	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	6	59	theme	random	1329:1334	arg1	type					1345:1348	the random fracture type	1325:1348	the random fracture type of fucoidan due to their different structure	1325:1393	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	6	60	from	cleavage	1185:1192	arg1	acid					1258:1261	glucuronic acid	1247:1261	glucuronic acid	1247:1261	The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure.					
30904529	1	61	theme	undesired	159:167	arg1	activities					185:194	undesired pharmacological activities	159:194	undesired pharmacological activities	159:194	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	2	62	from	5-6	599:601	arg1	control					582:588	control	582:588	control of pH at 5-6	582:601	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	2	62	from	5-6	599:601	arg1	pH					593:594	pH	593:594	pH at 5-6	593:601	Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight.					
30904529	1	63	dep	fucoidan	233:240	arg1	the					229:231	the	229:231	the	229:231	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	7	64	from	depolymerization	1438:1453	arg1	cucumber					1505:1512	sea cucumber	1501:1512	sea cucumber	1501:1512	These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure.					
30904529	1	65	theme	pharmacological	169:183	arg1	activities					185:194	undesired pharmacological activities	159:194	undesired pharmacological activities	159:194	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	0	66	theme	fucoidan	75:82	arg1	depolymerization					27:42	hydrothermal depolymerization	14:42	hydrothermal depolymerization	14:42	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	0	66	theme	fucoidan	75:82	arg1	profile					64:70	oligosaccharide profile	48:70	oligosaccharide profile	48:70	Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana.					
30904529	1	67	theme	hydrothermal	388:399	arg1	depolymerization					401:416	hydrothermal depolymerization	388:416	hydrothermal depolymerization	388:416	To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed.					
30904529	4	68	theme	optimized	848:856	arg1	fucoidan					885:892	the optimized hydrothermal depolymerized fucoidan	844:892	the optimized hydrothermal depolymerized fucoidan	844:892	The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged.					
30909000	0	0	theme	adsorption-desorption	107:127	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	1	1	theme	hydrothermal-assisted	379:399	arg1	approaches					410:419	facile hydrothermal-assisted assembly approaches	372:419	facile hydrothermal-assisted assembly approaches	372:419	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	7	2	theme	y	1629:1629	arg1	composites					1631:1640	GO-MAx:y composites	1622:1640	GO-MAx:y composites	1622:1640	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	7	2	theme	y	1629:1629	arg1	adsorbents					1668:1677	reusable adsorbents	1659:1677	reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions	1659:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	5	3	theme	adsorption	1270:1279	arg1	capacities					1281:1290	The adsorption capacities	1266:1290	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+	1266:1412	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	3	theme	adsorption	1270:1279	arg1	84.76					1419:1423	84.76	1419:1423	84.76	1419:1423	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	0	4	with	mechanism	198:206	arg1	pore-sizes					87:96	controllable pore-sizes	74:96	controllable pore-sizes	74:96	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	0	4	with	mechanism	198:206	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	3	5	theme	adsorbate	845:853	arg1	time					818:821	contact time	810:821	contact time	810:821	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	5	theme	adsorbate	845:853	arg1	concentration					855:867	initial adsorbate concentration	837:867	initial adsorbate concentration	837:867	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	0	6	theme	good	102:105	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	1	7	theme	assembly	401:408	arg1	approaches					410:419	facile hydrothermal-assisted assembly approaches	372:419	facile hydrothermal-assisted assembly approaches	372:419	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	1	8	theme	controllable	296:307	arg1	composites					320:329	controllable pore-sizes composites	296:329	controllable pore-sizes composites in the range of 6-40 nm	296:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	3	9	from	effects	799:805	arg1	process					913:919	the adsorption process	898:919	the adsorption process	898:919	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	7	10	theme	GO-MAx	1622:1627	arg1	composites					1631:1640	GO-MAx:y composites	1622:1640	GO-MAx:y composites	1622:1640	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	7	10	theme	GO-MAx	1622:1627	arg1	adsorbents					1668:1677	reusable adsorbents	1659:1677	reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions	1659:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	2	11	dep	transform	668:676	arg1	infrared					678:685	infrared	678:685	transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS)	668:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	1	12	theme	pore-sizes	309:318	arg1	composites					320:329	controllable pore-sizes composites	296:329	controllable pore-sizes composites in the range of 6-40 nm	296:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	4	13	theme	adsorption	949:958	arg1	capacities					960:969	The adsorption capacities	945:969	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR)	945:1186	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	13	theme	adsorption	949:958	arg1	22.17					1193:1197	22.17	1193:1197	22.17	1193:1197	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	14	theme	alizarin	1146:1153	arg1	ARS					1162:1164	ARS	1162:1164	ARS	1162:1164	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	14	theme	alizarin	1146:1153	arg1	S					1159:1159	alizarin red S	1146:1159	alizarin red S (ARS)	1146:1165	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	3	15	theme	initial	837:843	arg1	time					818:821	contact time	810:821	contact time	810:821	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	15	theme	initial	837:843	arg1	concentration					855:867	initial adsorbate concentration	837:867	initial adsorbate concentration	837:867	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	16	theme	solution	886:893	arg1	value					873:877	pH value	870:877	pH value of the solution	870:893	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	16	theme	solution	886:893	arg1	time					818:821	contact time	810:821	contact time	810:821	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	6	17	theme	y	1531:1531	arg1	composites					1533:1542	GO-MAx:y composites	1524:1542	GO-MAx:y composites	1524:1542	In addition, GO-MAx:y composites also exhibited high mechanical properties and good reusability.					
30909000	1	18	theme	Three-dimensional	209:225	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	0	19	theme	Facile	141:146	arg1	fabrication					148:158	Facile fabrication	141:158	Facile fabrication	141:158	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	2	20	theme	adsorption-desorption	627:647	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	20	theme	adsorption-desorption	627:647	arg1	Fourier					660:666	Fourier	660:666	Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS)	660:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	20	theme	adsorption-desorption	627:647	arg1	isotherms					649:657	N2 adsorption-desorption isotherms	624:657	N2 adsorption-desorption isotherms	624:657	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	0	21	with	fabrication	148:158	arg1	pore-sizes					87:96	controllable pore-sizes	74:96	controllable pore-sizes	74:96	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	0	21	with	fabrication	148:158	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	2	22	dep	morphologies	426:437	arg1	characterized					571:583	characterized	571:583	were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS)	566:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	3	23	theme	adsorption	902:911	arg1	process					913:919	the adsorption process	898:919	the adsorption process	898:919	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	4	24	theme	3D	974:975	arg1	composite					987:995	3D GO-MA20:1 composite	974:995	3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR)	974:1186	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	0	25	theme	porous	18:23	arg1	graphene					25:32	Three-dimensional porous graphene	0:32	Three-dimensional porous graphene	0:32	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	6	26	theme	GO-MAx	1524:1529	arg1	composites					1533:1542	GO-MAx:y composites	1524:1542	GO-MAx:y composites	1524:1542	In addition, GO-MAx:y composites also exhibited high mechanical properties and good reusability.					
30909000	2	27	theme	N2	624:625	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	27	theme	N2	624:625	arg1	Fourier					660:666	Fourier	660:666	Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS)	660:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	27	theme	N2	624:625	arg1	isotherms					649:657	N2 adsorption-desorption isotherms	624:657	N2 adsorption-desorption isotherms	624:657	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	4	28	theme	composite	987:995	arg1	capacities					960:969	The adsorption capacities	945:969	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR)	945:1186	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	28	theme	composite	987:995	arg1	22.17					1193:1197	22.17	1193:1197	22.17	1193:1197	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	7	29	theme	inorganic/organic	1702:1718	arg1	substances					1720:1729	removal/enrichment inorganic/organic substances	1683:1729	removal/enrichment inorganic/organic substances in aqueous solutions	1683:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	0	30	theme	Three-dimensional	0:16	arg1	graphene					25:32	Three-dimensional porous graphene	0:32	Three-dimensional porous graphene	0:32	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	2	31	theme	pore	440:443	arg1	sizes					445:449	pore sizes	440:449	pore sizes	440:449	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	3	32	theme	adsorption-desorption	766:786	arg1	mechanism					788:796	the adsorption-desorption mechanism	762:796	the adsorption-desorption mechanism	762:796	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	2	33	dep	ratios	553:558	arg1	x					561:561	x	561:561	x:y	561:563	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	6	34	theme	mechanical	1564:1573	arg1	properties					1575:1584	high mechanical properties	1559:1584	high mechanical properties	1559:1584	In addition, GO-MAx:y composites also exhibited high mechanical properties and good reusability.					
30909000	7	35	theme	reusable	1659:1666	arg1	composites					1631:1640	GO-MAx:y composites	1622:1640	GO-MAx:y composites	1622:1640	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	7	35	theme	reusable	1659:1666	arg1	adsorbents					1668:1677	reusable adsorbents	1659:1677	reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions	1659:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	4	36	theme	organic	1001:1007	arg1	contaminants					1009:1020	organic contaminants	1001:1020	organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR)	1001:1186	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	36	theme	organic	1001:1007	arg1	NR					1184:1185	NR	1184:1185	NR	1184:1185	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	1	37	theme	porous	232:237	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	4	38	theme	tert-butyl	1032:1041	arg1	TBHQ					1057:1060	TBHQ	1057:1060	TBHQ	1057:1060	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	38	theme	tert-butyl	1032:1041	arg1	hydroquinone					1043:1054	tert-butyl hydroquinone	1032:1054	tert-butyl hydroquinone (TBHQ)	1032:1061	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	1	39	from	composites	320:329	arg1	range					338:342	the range	334:342	the range of 6-40 nm	334:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	1	40	theme	graphene	239:246	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	2	41	dep	x	561:561	arg1	y					563:563	y	563:563	x:y	561:563	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	5	42	theme	GO-MA30:1	1295:1303	arg1	composite					1305:1313	GO-MA30:1 composite	1295:1313	GO-MA30:1 composite	1295:1313	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	1	43	theme	oxide-maize	248:258	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	0	44	theme	amylopectin	46:56	arg1	composites					58:67	amylopectin composites	46:67	amylopectin composites with controllable pore-sizes and good adsorption-desorption properties	46:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	1	45	theme	6-40 nm	347:353	arg1	range					338:342	the range	334:342	the range of 6-40 nm	334:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	2	46	theme	y	507:507	arg1	composites					509:518	y composites	507:518	y composites with	507:523	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	4	47	theme	red	1155:1157	arg1	ARS					1162:1164	ARS	1162:1164	ARS	1162:1164	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	4	47	theme	red	1155:1157	arg1	S					1159:1159	alizarin red S	1146:1159	alizarin red S (ARS)	1146:1165	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	1	48	theme	amylopectin	260:270	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	0	49	theme	controllable	74:85	arg1	pore-sizes					87:96	controllable pore-sizes	74:96	controllable pore-sizes	74:96	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	6	50	theme	good	1590:1593	arg1	reusability					1595:1605	good reusability	1590:1605	good reusability	1590:1605	In addition, GO-MAx:y composites also exhibited high mechanical properties and good reusability.					
30909000	0	51	theme	adsorption	187:196	arg1	mechanism					198:206	the adsorption mechanism	183:206	the adsorption mechanism	183:206	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	4	52	theme	GO-MA20:1	977:985	arg1	composite					987:995	3D GO-MA20:1 composite	974:995	3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR)	974:1186	The adsorption capacities of 3D GO-MA20:1 composite for organic contaminants including tert-butyl hydroquinone (TBHQ), p-aminophenol (PAP), p-nitrophenol (PNP), o-nitrophenol (MNP), hydroquinone (HQ), alizarin red S (ARS) and neutral red (NR) were 22.17, 116.4, 44.78, 36.96, 16.10, 39.92 and 24.23 mg g-1, respectively.					
30909000	1	53	dep	Three-dimensional	209:225	arg1	3D					228:229	3D	228:229	3D	228:229	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	2	54	theme	photoelectron	718:730	arg1	XPS					746:748	XPS	746:748	XPS	746:748	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	54	theme	photoelectron	718:730	arg1	spectroscopy					732:743	X-ray photoelectron spectroscopy	712:743	X-ray photoelectron spectroscopy (XPS)	712:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	55	theme	GO-MAx	500:505	arg1	compositions					484:495	compositions	484:495	compositions of GO-MAx	484:505	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	55	theme	GO-MAx	500:505	arg1	area					469:472	specific surface area	452:472	specific surface area (SSA)	452:478	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	55	theme	GO-MAx	500:505	arg1	morphologies					426:437	The morphologies	422:437	The morphologies	422:437	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	55	theme	GO-MAx	500:505	arg1	sizes					445:449	pore sizes	440:449	pore sizes	440:449	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	55	theme	GO-MAx	500:505	arg1	SSA					475:477	SSA	475:477	SSA	475:477	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	56	theme	specific	452:459	arg1	area					469:472	specific surface area	452:472	specific surface area (SSA)	452:478	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	56	theme	specific	452:459	arg1	SSA					475:477	SSA	475:477	SSA	475:477	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	57	theme	scanning	588:595	arg1	SEM					618:620	SEM	618:620	SEM	618:620	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	57	theme	scanning	588:595	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	57	theme	scanning	588:595	arg1	isotherms					649:657	N2 adsorption-desorption isotherms	624:657	N2 adsorption-desorption isotherms	624:657	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	58	theme	X-ray	712:716	arg1	XPS					746:748	XPS	746:748	XPS	746:748	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	58	theme	X-ray	712:716	arg1	spectroscopy					732:743	X-ray photoelectron spectroscopy	712:743	X-ray photoelectron spectroscopy (XPS)	712:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	7	59	used	used	1651:1654	arg2	adsorbents					1668:1677	reusable adsorbents	1659:1677	reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions	1659:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	7	59	used	used	1651:1654	arg2	composites					1631:1640	GO-MAx:y composites	1622:1640	GO-MAx:y composites	1622:1640	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	5	60	theme	composite	1305:1313	arg1	capacities					1281:1290	The adsorption capacities	1266:1290	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+	1266:1412	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	60	theme	composite	1305:1313	arg1	84.76					1419:1423	84.76	1419:1423	84.76	1419:1423	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	6	61	theme	high	1559:1562	arg1	properties					1575:1584	high mechanical properties	1559:1584	high mechanical properties	1559:1584	In addition, GO-MAx:y composites also exhibited high mechanical properties and good reusability.					
30909000	2	62	theme	mass	548:551	arg1	ratios					553:558	different GO-to-MA mass ratios	529:558	different GO-to-MA mass ratios (x:y)	529:564	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	5	63	theme	inorganic	1319:1327	arg1	Er3+					1409:1412	Er3+	1409:1412	Er3+	1409:1412	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Pb2+					1350:1353	Pb2+	1350:1353	Pb2+	1350:1353	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Cd2+					1371:1374	Cd2+	1371:1374	Cd2+	1371:1374	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Cu2+					1377:1380	Cu2+	1377:1380	Cu2+	1377:1380	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Y3+					1395:1397	Y3+	1395:1397	Y3+	1395:1397	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Mn2+					1356:1359	Mn2+	1356:1359	Mn2+	1356:1359	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Yb3+					1400:1403	Yb3+	1400:1403	Yb3+	1400:1403	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Cr2O72-					1362:1368	Cr2O72-	1362:1368	Cr2O72-	1362:1368	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	Nd3+					1383:1386	Nd3+	1383:1386	Nd3+	1383:1386	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	substances					1329:1338	inorganic substances	1319:1338	inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+	1319:1412	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	5	63	theme	inorganic	1319:1327	arg1	La3+					1389:1392	La3+	1389:1392	La3+	1389:1392	The adsorption capacities of GO-MA30:1 composite for inorganic substances including Pb2+, Mn2+, Cr2O72-, Cd2+, Cu2+, Nd3+, La3+, Y3+, Yb3+ and Er3+ were 84.76, 7.92, 13.6, 17.64, 30.56, 25.52, 12.48, 16.96, 23.32 and 30.32 mg g-1, respectively.					
30909000	2	64	theme	electron	597:604	arg1	SEM					618:620	SEM	618:620	SEM	618:620	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	64	theme	electron	597:604	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	64	theme	electron	597:604	arg1	isotherms					649:657	N2 adsorption-desorption isotherms	624:657	N2 adsorption-desorption isotherms	624:657	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	7	65	theme	removal/enrichment	1683:1700	arg1	substances					1720:1729	removal/enrichment inorganic/organic substances	1683:1729	removal/enrichment inorganic/organic substances in aqueous solutions	1683:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	7	66	theme	aqueous	1734:1740	arg1	solutions					1742:1750	aqueous solutions	1734:1750	aqueous solutions	1734:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	0	67	with	reutilization	164:176	arg1	pore-sizes					87:96	controllable pore-sizes	74:96	controllable pore-sizes	74:96	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	0	67	with	reutilization	164:176	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	1	68	theme	GO-MA	273:277	arg1	composites					280:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites	209:289	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm	209:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	2	69	theme	GO-to-MA	539:546	arg1	ratios					553:558	different GO-to-MA mass ratios	529:558	different GO-to-MA mass ratios (x:y)	529:564	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	70	theme	surface	461:467	arg1	area					469:472	specific surface area	452:472	specific surface area (SSA)	452:478	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	2	70	theme	surface	461:467	arg1	SSA					475:477	SSA	475:477	SSA	475:477	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	1	71	with	composites	280:289	arg1	composites					320:329	controllable pore-sizes composites	296:329	controllable pore-sizes composites in the range of 6-40 nm	296:353	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	7	72	from	substances	1720:1729	arg1	solutions					1742:1750	aqueous solutions	1734:1750	aqueous solutions	1734:1750	Consequently, GO-MAx:y composites could be used as reusable adsorbents for removal/enrichment inorganic/organic substances in aqueous solutions.					
30909000	3	73	theme	pH	870:871	arg1	value					873:877	pH value	870:877	pH value of the solution	870:893	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	73	theme	pH	870:871	arg1	time					818:821	contact time	810:821	contact time	810:821	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	74	theme	contact	810:816	arg1	value					873:877	pH value	870:877	pH value of the solution	870:893	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	74	theme	contact	810:816	arg1	temperature					824:834	temperature	824:834	temperature	824:834	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	74	theme	contact	810:816	arg1	time					818:821	contact time	810:821	contact time	810:821	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	3	74	theme	contact	810:816	arg1	concentration					855:867	initial adsorbate concentration	837:867	initial adsorbate concentration	837:867	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
30909000	2	75	theme	different	529:537	arg1	ratios					553:558	different GO-to-MA mass ratios	529:558	different GO-to-MA mass ratios (x:y)	529:564	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	1	76	theme	facile	372:377	arg1	approaches					410:419	facile hydrothermal-assisted assembly approaches	372:419	facile hydrothermal-assisted assembly approaches	372:419	Three-dimensional (3D) porous graphene oxide-maize amylopectin (GO-MA) composites with controllable pore-sizes composites in the range of 6-40 nm were prepared by facile hydrothermal-assisted assembly approaches.					
30909000	0	77	with	composites	58:67	arg1	pore-sizes					87:96	controllable pore-sizes	74:96	controllable pore-sizes	74:96	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	0	77	with	composites	58:67	arg1	properties					129:138	good adsorption-desorption properties	102:138	good adsorption-desorption properties	102:138	Three-dimensional porous graphene oxide-maize amylopectin composites with controllable pore-sizes and good adsorption-desorption properties: Facile fabrication and reutilization, and the adsorption mechanism.					
30909000	2	78	dep	Fourier	660:666	arg1	transform					668:676	transform	668:676	transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS)	668:749	The morphologies, pore sizes, specific surface area (SSA) and compositions of GO-MAx:y composites with and different GO-to-MA mass ratios (x:y) were characterized by scanning electron microscopy (SEM), N2 adsorption-desorption isotherms, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS).					
30909000	3	79	theme	time	818:821	arg1	effects					799:805	effects	799:805	effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process	799:919	To reveal the adsorption-desorption mechanism, effects of contact time, temperature, initial adsorbate concentration, pH value of the solution on the adsorption process were studied in detail.					
31639407	1	0	theme	-β-d-Fru-	357:365	arg1	-α-d-Rha					373:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	0	theme	-β-d-Fru-	357:365	arg1	[β-d-Glc-					327:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-	271:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	271:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	2	1	from	C-3	427:429	arg1	C-2					491:493	C-2	491:493	C-2	491:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	1	2	theme	crocosmiiflora	156:169	arg1	flowers					184:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	3	theme	new	236:238	arg1	tetrasaccharide					254:268	a new sucrose-based tetrasaccharide	234:268	a new sucrose-based tetrasaccharide	234:268	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	3	theme	new	236:238	arg1	[β-d-Glc-					327:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-	271:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	271:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	0	4	theme	tetrasaccharide	74:88	arg1	esters					44:49	antibacterial p-coumaroyl esters	18:49	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	5	5	theme	TLC	852:854	arg1	sites					871:875	TLC, and acylation sites	852:875	TLC, and acylation sites	852:875	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	1	6	theme	sucrose-based	240:252	arg1	tetrasaccharide					254:268	a new sucrose-based tetrasaccharide	234:268	a new sucrose-based tetrasaccharide	234:268	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	6	theme	sucrose-based	240:252	arg1	[β-d-Glc-					327:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-	271:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	271:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	0	7	theme	×	105:105	arg1	flowers					135:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	2	8	from	C-4	447:449	arg1	C-2					491:493	C-2	491:493	C-2	491:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	1	9	theme	tetrasaccharide	254:268	arg1	esters					205:210	four esters	200:210	four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	200:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	9	theme	tetrasaccharide	254:268	arg1	A-D					226:228	montbresides A-D	213:228	montbresides A-D	213:228	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	0	10	theme	Crocosmia	95:103	arg1	flowers					135:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	4	11	from	1D	753:754	arg1	data					727:730	MS data	724:730	MS data	724:730	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	11	from	1D	753:754	arg1	structures					737:746	structures	737:746	structures	737:746	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	12	theme	MS	724:725	arg1	data					727:730	MS data	724:730	MS data	724:730	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	6	13	theme	enzymic	933:939	arg1	digestion					941:949	enzymic digestion	933:949	enzymic digestion	933:949	Enantiomers were distinguished by enzymic digestion.					
31639407	8	14	theme	concentration	1181:1193	arg1	~6 μg/ml					1195:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	8	14	theme	concentration	1181:1193	arg1	aureus					1154:1159	Staphylococcus aureus	1139:1159	Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml)	1139:1203	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	2	15	theme	fructose	526:533	arg1	C-4					447:449	C-4	447:449	C-4 of α-glucose, plus O-acetyl residues on C-2	447:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	15	theme	fructose	526:533	arg1	C-6					519:521	C-6	519:521	C-6 of fructose	519:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	15	theme	fructose	526:533	arg1	C-3					427:429	C-3	427:429	C-3 of fructose	427:441	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	15	theme	fructose	526:533	arg1	C-3					499:501	C-3	499:501	C-3	499:501	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	5	16	theme	selective	821:829	arg1	spectra					840:846	selective 1D TOCSY spectra	821:846	selective 1D TOCSY spectra	821:846	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	1	17	theme	montbretia	172:181	arg1	flowers					184:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	0	18	theme	crocosmiiflora	107:120	arg1	flowers					135:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	0	19	from	esters	44:49	arg1	flowers					135:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	7	20	theme	purified	1038:1045	arg1	montbresides					1047:1058	purified montbresides	1038:1058	purified montbresides	1038:1058	Montbretia flower extracts were cytotoxic against six human cancerous cell-lines, but purified montbresides lacked cytotoxicity.					
31639407	2	21	from	C-6	519:521	arg1	C-2					491:493	C-2	491:493	C-2	491:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	4	22	from	montbresides	638:649	arg1	trans-					628:633	trans-	628:633	trans-	628:633	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	22	from	montbresides	638:649	arg1	p-coumaroyls					611:622	The p-coumaroyls	607:622	The p-coumaroyls	607:622	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	23	dep	1D	753:754	arg1	spectra					767:773	NMR spectra	763:773	NMR spectra	763:773	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	2	24	theme	residues	479:486	arg1	C-4					447:449	C-4	447:449	C-4 of α-glucose, plus O-acetyl residues on C-2	447:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	24	theme	residues	479:486	arg1	C-6					519:521	C-6	519:521	C-6 of fructose	519:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	24	theme	residues	479:486	arg1	C-3					427:429	C-3	427:429	C-3 of fructose	427:441	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	24	theme	residues	479:486	arg1	C-3					499:501	C-3	499:501	C-3	499:501	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	8	25	theme	inhibitory	1170:1179	arg1	~6 μg/ml					1195:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	8	25	theme	inhibitory	1170:1179	arg1	aureus					1154:1159	Staphylococcus aureus	1139:1159	Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml)	1139:1203	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	5	26	theme	2D	882:883	arg1	spectra					890:896	2D HMBC spectra	882:896	2D HMBC spectra	882:896	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	5	27	theme	1D	831:832	arg1	spectra					840:846	selective 1D TOCSY spectra	821:846	selective 1D TOCSY spectra	821:846	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	0	28	theme	Montbresides	0:11	arg1	A-D					13:15	Montbresides A-D	0:15	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	1	29	theme	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose	271:325	arg1	-α-d-Rha					373:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	29	theme	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose	271:325	arg1	tetrasaccharide					254:268	a new sucrose-based tetrasaccharide	234:268	a new sucrose-based tetrasaccharide	234:268	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	29	theme	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose	271:325	arg1	[β-d-Glc-					327:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-	271:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	271:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	9	30	theme	potential	1222:1230	arg1	Montbretia					1206:1215	Montbretia	1206:1215	Montbretia	1206:1215	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	9	30	theme	potential	1222:1230	arg1	source					1232:1237	a potential source	1220:1237	a potential source of new cytotoxins and antibacterial agents	1220:1280	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	5	31	theme	HMBC	885:888	arg1	spectra					890:896	2D HMBC spectra	882:896	2D HMBC spectra	882:896	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	4	32	from	trans-	628:633	arg1	montbresides					638:649	montbresides A and C	638:657	montbresides A and C	638:657	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	32	from	trans-	628:633	arg1	C					657:657	C	657:657	C	657:657	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	32	from	trans-	628:633	arg1	A					651:651	A	651:651	A	651:651	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	32	from	trans-	628:633	arg1	D.					677:678	D.	677:678	D.	677:678	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	32	from	trans-	628:633	arg1	B					671:671	B	671:671	B	671:671	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	5	33	theme	TOCSY	834:838	arg1	spectra					840:846	selective 1D TOCSY spectra	821:846	selective 1D TOCSY spectra	821:846	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	0	34	theme	antibacterial	18:30	arg1	esters					44:49	antibacterial p-coumaroyl esters	18:49	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	4	35	dep	montbresides	638:649	arg1	montbresides					638:649	montbresides A and C	638:657	montbresides A and C	638:657	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	35	dep	montbresides	638:649	arg1	C					657:657	C	657:657	C	657:657	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	35	dep	montbresides	638:649	arg1	A					651:651	A	651:651	A	651:651	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	7	36	theme	Montbretia	952:961	arg1	extracts					970:977	Montbretia flower extracts	952:977	Montbretia flower extracts	952:977	Montbretia flower extracts were cytotoxic against six human cancerous cell-lines, but purified montbresides lacked cytotoxicity.					
31639407	5	37	theme	Monosaccharide	776:789	arg1	residues					791:798	Monosaccharide residues	776:798	Monosaccharide residues	776:798	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	2	38	theme	fructose	434:441	arg1	C-4					447:449	C-4	447:449	C-4 of α-glucose, plus O-acetyl residues on C-2	447:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	38	theme	fructose	434:441	arg1	C-6					519:521	C-6	519:521	C-6 of fructose	519:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	38	theme	fructose	434:441	arg1	C-3					427:429	C-3	427:429	C-3 of fructose	427:441	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	38	theme	fructose	434:441	arg1	C-3					499:501	C-3	499:501	C-3	499:501	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	0	39	theme	montbretia	123:132	arg1	flowers					135:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Crocosmia × crocosmiiflora (montbretia) flowers	95:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	4	40	dep	B	671:671	arg1	determined					708:717	determined	708:717	were determined from MS data, and structures from 1D and 2D NMR spectra	703:773	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	3	41	dep	Montbresides	536:547	arg1	A					549:549	A	549:549	A	549:549	Montbresides A and B are additionally O-acetylated on C-1 of fructose.					
31639407	3	41	dep	Montbresides	536:547	arg1	Montbresides					536:547	Montbresides A and B	536:555	Montbresides A and B	536:555	Montbresides A and B are additionally O-acetylated on C-1 of fructose.					
31639407	3	41	dep	Montbresides	536:547	arg1	B					555:555	B	555:555	B	555:555	Montbresides A and B are additionally O-acetylated on C-1 of fructose.					
31639407	2	42	theme	O-acetyl	470:477	arg1	residues					479:486	α-glucose, plus O-acetyl residues	454:486	α-glucose, plus O-acetyl residues	454:486	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	9	43	theme	new	1242:1244	arg1	cytotoxins					1246:1255	new cytotoxins	1242:1255	new cytotoxins	1242:1255	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	4	44	theme	NMR	763:765	arg1	spectra					767:773	NMR spectra	763:773	NMR spectra	763:773	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	0	45	theme	p-coumaroyl	32:42	arg1	esters					44:49	antibacterial p-coumaroyl esters	18:49	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	9	46	theme	cytotoxins	1246:1255	arg1	Montbretia					1206:1215	Montbretia	1206:1215	Montbretia	1206:1215	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	9	46	theme	cytotoxins	1246:1255	arg1	source					1232:1237	a potential source	1220:1237	a potential source of new cytotoxins and antibacterial agents	1220:1280	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	2	47	contain	possess	393:399	arg1	All					384:386	All	384:386	All	384:386	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	47	contain	possess	393:399	arg2	residues					415:422	O-p-coumaroyl residues	401:422	O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose	401:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	1	48	theme	montbresides	213:224	arg1	esters					205:210	four esters	200:210	four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	200:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	48	theme	montbresides	213:224	arg1	A-D					226:228	montbresides A-D	213:228	montbresides A-D	213:228	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	49	theme	-α-d-Glc-	343:351	arg1	-α-d-Rha					373:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha	337:380	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	49	theme	-α-d-Glc-	343:351	arg1	[β-d-Glc-					327:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-	271:335	3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha]	271:381	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	7	50	theme	human	1006:1010	arg1	cell-lines					1022:1031	six human cancerous cell-lines	1002:1031	six human cancerous cell-lines	1002:1031	Montbretia flower extracts were cytotoxic against six human cancerous cell-lines, but purified montbresides lacked cytotoxicity.					
31639407	2	51	theme	α-glucose	454:462	arg1	residues					479:486	α-glucose, plus O-acetyl residues	454:486	α-glucose, plus O-acetyl residues	454:486	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	52	theme	O-p-coumaroyl	401:413	arg1	residues					415:422	O-p-coumaroyl residues	401:422	O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose	401:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	7	53	theme	cancerous	1012:1020	arg1	cell-lines					1022:1031	six human cancerous cell-lines	1002:1031	six human cancerous cell-lines	1002:1031	Montbretia flower extracts were cytotoxic against six human cancerous cell-lines, but purified montbresides lacked cytotoxicity.					
31639407	9	54	theme	antibacterial	1261:1273	arg1	agents					1275:1280	antibacterial agents	1261:1280	antibacterial agents	1261:1280	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	9	55	theme	agents	1275:1280	arg1	Montbretia					1206:1215	Montbretia	1206:1215	Montbretia	1206:1215	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	9	55	theme	agents	1275:1280	arg1	source					1232:1237	a potential source	1220:1237	a potential source of new cytotoxins and antibacterial agents	1220:1280	Montbretia is a potential source of new cytotoxins and antibacterial agents.					
31639407	7	56	theme	flower	963:968	arg1	extracts					970:977	Montbretia flower extracts	952:977	Montbretia flower extracts	952:977	Montbretia flower extracts were cytotoxic against six human cancerous cell-lines, but purified montbresides lacked cytotoxicity.					
31639407	5	57	theme	acylation	861:869	arg1	sites					871:875	TLC, and acylation sites	852:875	TLC, and acylation sites	852:875	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	5	58	from	spectra	890:896	arg1	spectra					840:846	selective 1D TOCSY spectra	821:846	selective 1D TOCSY spectra	821:846	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	5	58	from	spectra	890:896	arg1	sites					871:875	TLC, and acylation sites	852:875	TLC, and acylation sites	852:875	Monosaccharide residues were identified from selective 1D TOCSY spectra and TLC, and acylation sites from 2D HMBC spectra.					
31639407	1	59	dep	-α-d-Rha	373:380	arg1	4 ← 1					367:371	4 ← 1	367:371	4 ← 1	367:371	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	1	59	dep	-α-d-Rha	373:380	arg1	1↔2					353:355	1↔2	353:355	1↔2	353:355	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	8	60	theme	antibacterial	1108:1120	arg1	activity					1122:1129	antibacterial activity	1108:1129	antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml)	1108:1203	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	2	61	from	C-3	499:501	arg1	C-2					491:493	C-2	491:493	C-2	491:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	3	62	theme	fructose	597:604	arg1	C-1					590:592	C-1	590:592	C-1 of fructose	590:604	Montbresides A and B are additionally O-acetylated on C-1 of fructose.					
31639407	4	63	from	2D	760:761	arg1	data					727:730	MS data	724:730	MS data	724:730	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	63	from	2D	760:761	arg1	structures					737:746	structures	737:746	structures	737:746	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	64	theme	Elemental	680:688	arg1	compositions					690:701	Elemental compositions	680:701	Elemental compositions	680:701	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	65	from	D.	677:678	arg1	trans-					628:633	trans-	628:633	trans-	628:633	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	65	from	D.	677:678	arg1	p-coumaroyls					611:622	The p-coumaroyls	607:622	The p-coumaroyls	607:622	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	8	66	theme	minimal	1162:1168	arg1	~6 μg/ml					1195:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	minimal inhibitory concentration ~6 μg/ml	1162:1202	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	8	66	theme	minimal	1162:1168	arg1	aureus					1154:1159	Staphylococcus aureus	1139:1159	Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml)	1139:1203	Each montbreside displayed antibacterial activity against Staphylococcus aureus (minimal inhibitory concentration ~6 μg/ml).					
31639407	0	67	from	flowers	135:141	arg1	tetrasaccharide					74:88	a new sucrose-based tetrasaccharide	54:88	a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers	54:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	0	67	from	flowers	135:141	arg1	esters					44:49	antibacterial p-coumaroyl esters	18:49	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	0	68	theme	sucrose-based	60:72	arg1	tetrasaccharide					74:88	a new sucrose-based tetrasaccharide	54:88	a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers	54:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	0	69	dep	A-D	13:15	arg1	esters					44:49	antibacterial p-coumaroyl esters	18:49	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.	0:142	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	2	70	from	residues	415:422	arg1	C-4					447:449	C-4	447:449	C-4 of α-glucose, plus O-acetyl residues on C-2	447:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	70	from	residues	415:422	arg1	C-6					519:521	C-6	519:521	C-6 of fructose	519:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	70	from	residues	415:422	arg1	C-3					427:429	C-3	427:429	C-3 of fructose	427:441	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	1	71	theme	Crocosmia	144:152	arg1	flowers					184:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	4	72	from	B	671:671	arg1	trans-					628:633	trans-	628:633	trans-	628:633	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	4	72	from	B	671:671	arg1	p-coumaroyls					611:622	The p-coumaroyls	607:622	The p-coumaroyls	607:622	The p-coumaroyls are trans- in montbresides A and C and cis- in B and D. Elemental compositions were determined from MS data, and structures from 1D and 2D NMR spectra.					
31639407	0	73	theme	new	56:58	arg1	tetrasaccharide					74:88	a new sucrose-based tetrasaccharide	54:88	a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers	54:141	Montbresides A-D: antibacterial p-coumaroyl esters of a new sucrose-based tetrasaccharide from Crocosmia × crocosmiiflora (montbretia) flowers.					
31639407	1	74	theme	×	154:154	arg1	flowers					184:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers	144:190	Crocosmia × crocosmiiflora (montbretia) flowers yielded four esters (montbresides A-D) of a new sucrose-based tetrasaccharide, 3-O-β-d-glucopyranosyl-4´-O-α-d-rhamnopyranosyl-sucrose [β-d-Glc-(1 → 3)-α-d-Glc-(1↔2)-β-d-Fru-(4 ← 1)-α-d-Rha].					
31639407	2	75	theme	rhamnose	506:513	arg1	C-4					447:449	C-4	447:449	C-4 of α-glucose, plus O-acetyl residues on C-2	447:493	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	75	theme	rhamnose	506:513	arg1	C-6					519:521	C-6	519:521	C-6 of fructose	519:533	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	75	theme	rhamnose	506:513	arg1	C-3					427:429	C-3	427:429	C-3 of fructose	427:441	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
31639407	2	75	theme	rhamnose	506:513	arg1	C-3					499:501	C-3	499:501	C-3	499:501	All four possess O-p-coumaroyl residues on C-3 of fructose and C-4 of α-glucose, plus O-acetyl residues on C-2 and C-3 of rhamnose and C-6 of fructose.					
30915778	0	0	theme	ovary	173:177	arg1	syndrome					179:186	polycystic ovary syndrome	162:186	polycystic ovary syndrome	162:186	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	2	1	with	association	627:637	arg1	g					651:651	MI 1.1 g	644:651	MI 1.1 g	644:651	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	1	with	association	627:637	arg1	group					712:716	group 3	712:718	group 3	712:718	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	1	with	association	627:637	arg1	mg					682:683	D-chiroinositol (DCI) 27.6 mg	655:683	D-chiroinositol (DCI) 27.6 mg	655:683	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	0	2	with	women	151:155	arg1	syndrome					179:186	polycystic ovary syndrome	162:186	polycystic ovary syndrome	162:186	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	3	theme	polycystic	162:171	arg1	syndrome					179:186	polycystic ovary syndrome	162:186	polycystic ovary syndrome	162:186	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	4	dep	clinical	109:116	arg1	outcomes					139:146	outcomes	139:146	outcomes	139:146	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	4	5	theme	Body	951:954	arg1	weight					956:961	RESULTS Body weight	943:961	RESULTS Body weight	943:961	RESULTS Body weight, BMI, waist and hip circumferences decreased significantly in all groups.					
30915778	0	6	from	women	151:155	arg1	clinical					109:116	clinical	109:116	clinical	109:116	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	1	7	theme	clinical	341:348	arg1	terms					332:336	terms	332:336	terms of clinical and body composition outcomes	332:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	2	8	theme	acid	582:585	arg1	µg					591:592	folic acid 400 µg	576:592	folic acid 400 µg	576:592	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	6	9	theme	fat	1293:1295	arg1	reduction					1302:1310	the fat mass reduction	1289:1310	the fat mass reduction	1289:1310	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	6	10	theme	CONCLUSIONS	1205:1215	arg1	MI+DCI					1217:1222	CONCLUSIONS MI+DCI	1205:1222	CONCLUSIONS MI+DCI in association with diet	1205:1247	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	2	11	dep	Group	525:529	arg1	n					534:534	n = 21	534:539	n = 21	534:539	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	4	12	theme	RESULTS	943:949	arg1	weight					956:961	RESULTS Body weight	943:961	RESULTS Body weight	943:961	RESULTS Body weight, BMI, waist and hip circumferences decreased significantly in all groups.					
30915778	2	13	theme	folic	576:580	arg1	µg					591:592	folic acid 400 µg	576:592	folic acid 400 µg	576:592	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	14	theme	MI	644:645	arg1	g					651:651	MI 1.1 g	644:651	MI 1.1 g	644:651	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	14	theme	MI	644:645	arg1	group					712:716	group 3	712:718	group 3	712:718	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	1	15	theme	body	354:357	arg1	composition					359:369	body composition	354:369	body composition	354:369	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	3	16	theme	body	861:864	arg1	composition					866:876	body composition	861:876	body composition	861:876	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	17	theme	folic	687:691	arg1	µg					702:703	folic acid 400 µg	687:703	folic acid 400 µg	687:703	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	18	dep	group	601:605	arg1	n					610:610	n = 10	610:615	n = 10	610:615	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	19	theme	METHODS	394:400	arg1	patients					464:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	6	20	theme	lean	1346:1349	arg1	mass					1351:1354	percent lean mass	1338:1354	percent lean mass	1338:1354	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	1	21	theme	composition	359:369	arg1	terms					332:336	terms	332:336	terms of clinical and body composition outcomes	332:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	2	22	theme	overweight/obese	447:462	arg1	patients					464:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	6	23	theme	cycle	1447:1451	arg1	regularity					1419:1428	the regularity	1415:1428	the regularity of the menstrual cycle	1415:1451	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	3	24	theme	waist-hip	834:842	arg1	WHR					851:853	WHR	851:853	WHR	851:853	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	24	theme	waist-hip	834:842	arg1	ratio					844:848	waist-hip ratio	834:848	waist-hip ratio (WHR)	834:854	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	24	theme	waist-hip	834:842	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	6	25	theme	weight	1273:1278	arg1	loss					1280:1283	the weight loss	1269:1283	the weight loss	1269:1283	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	2	26	theme	PATIENTS	381:388	arg1	patients					464:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	1	27	theme	distinct	259:266	arg1	modalities					278:287	three distinct treatment modalities	253:287	three distinct treatment modalities	253:287	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	3	28	theme	hip	815:817	arg1	circumference					819:831	hip circumference	815:831	hip circumference	815:831	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	28	theme	hip	815:817	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	29	theme	D-chiroinositol	655:669	arg1	mg					682:683	D-chiroinositol (DCI) 27.6 mg	655:683	D-chiroinositol (DCI) 27.6 mg	655:683	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	1	30	from	benefits	320:327	arg1	terms					332:336	terms	332:336	terms of clinical and body composition outcomes	332:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	0	31	theme	treatment	17:25	arg1	myoinositol					60:70	myoinositol	60:70	myoinositol	60:70	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	31	theme	treatment	17:25	arg1	myoinositol					45:55	myoinositol	45:55	myoinositol	45:55	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	31	theme	treatment	17:25	arg1	diet					39:42	diet	39:42	diet	39:42	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	31	theme	treatment	17:25	arg1	modalities					27:36	three treatment modalities	11:36	three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol)	11:104	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	1	32	dep	clinical	341:348	arg1	outcomes					371:378	outcomes	371:378	outcomes	371:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	3	33	theme	waist	794:798	arg1	circumference					800:812	waist circumference	794:812	waist circumference	794:812	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	33	theme	waist	794:798	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	34	theme	Ferriman-Gallwey	747:762	arg1	score					764:768	Ferriman-Gallwey score	747:768	Ferriman-Gallwey score	747:768	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	34	theme	Ferriman-Gallwey	747:762	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	35	theme	syndrome	431:438	arg1	patients					464:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	6	36	theme	menstrual	1437:1445	arg1	cycle					1447:1451	the menstrual cycle	1433:1451	the menstrual cycle	1433:1451	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	3	37	theme	Menstrual	730:738	arg1	cycle					740:744	Menstrual cycle	730:744	Menstrual cycle	730:744	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	38	theme	ovary	425:429	arg1	PCOS					441:444	PCOS	441:444	PCOS	441:444	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	38	theme	ovary	425:429	arg1	syndrome					431:438	polycystic ovary syndrome	414:438	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	39	dep	diet	543:546	arg1	g					572:572	4 g	570:572	4 g	570:572	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	40	theme	=	723:723	arg1	n					721:721	n = 13	721:726	n = 13	721:726	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	41	from	diet	619:622	arg1	association					627:637	association	627:637	association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13)	627:727	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	6	42	theme	mass	1351:1354	arg1	increase					1326:1333	a slight increase	1317:1333	a slight increase of percent lean mass	1317:1354	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	2	43	theme	polycystic	414:423	arg1	PCOS					441:444	PCOS	441:444	PCOS	441:444	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	43	theme	polycystic	414:423	arg1	syndrome					431:438	polycystic ovary syndrome	414:438	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	0	44	theme	modalities	27:36	arg1	Effects					0:6	Effects	0:6	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.	0:187	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	45	theme	body	122:125	arg1	composition					127:137	body composition	122:137	body composition	122:137	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	2	46	theme	=	612:612	arg1	n					610:610	n = 10	610:615	n = 10	610:615	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	47	theme	Forty-three	402:412	arg1	patients					464:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients	381:471	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	1	48	theme	greatest	302:309	arg1	benefits					320:327	the greatest clinical benefits	298:327	the greatest clinical benefits in terms of clinical and body composition outcomes	298:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	1	49	theme	treatment	268:276	arg1	modalities					278:287	three distinct treatment modalities	253:287	three distinct treatment modalities	253:287	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	3	50	theme	mass	776:779	arg1	circumference					800:812	waist circumference	794:812	waist circumference	794:812	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	50	theme	mass	776:779	arg1	ratio					844:848	waist-hip ratio	834:848	waist-hip ratio (WHR)	834:854	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	50	theme	mass	776:779	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	50	theme	mass	776:779	arg1	BMI					788:790	BMI	788:790	BMI	788:790	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	50	theme	mass	776:779	arg1	circumference					819:831	hip circumference	815:831	hip circumference	815:831	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	50	theme	mass	776:779	arg1	score					764:768	Ferriman-Gallwey score	747:768	Ferriman-Gallwey score	747:768	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	51	dep	group	712:716	arg1	n					721:721	n = 13	721:726	n = 13	721:726	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	0	52	from	Effects	0:6	arg1	clinical					109:116	clinical	109:116	clinical	109:116	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	52	from	Effects	0:6	arg1	composition					127:137	body composition	122:137	body composition	122:137	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	1	53	theme	clinical	311:318	arg1	benefits					320:327	the greatest clinical benefits	298:327	the greatest clinical benefits in terms of clinical and body composition outcomes	298:378	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	6	54	with	association	1227:1237	arg1	diet					1244:1247	diet	1244:1247	diet	1244:1247	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	0	55	dep	modalities	27:36	arg1	myoinositol					60:70	myoinositol	60:70	myoinositol	60:70	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	55	dep	modalities	27:36	arg1	myoinositol					45:55	myoinositol	45:55	myoinositol	45:55	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	55	dep	modalities	27:36	arg1	diet					39:42	diet	39:42	diet	39:42	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	0	55	dep	modalities	27:36	arg1	modalities					27:36	three treatment modalities	11:36	three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol)	11:104	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
30915778	6	56	theme	slight	1319:1324	arg1	increase					1326:1333	a slight increase	1317:1333	a slight increase of percent lean mass	1317:1354	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	6	57	theme	percent	1338:1344	arg1	mass					1351:1354	percent lean mass	1338:1354	percent lean mass	1338:1354	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	5	58	dep	was	1043:1045	arg1	obtained					1163:1170	obtained	1163:1170	was obtained in all patients only in-group 3	1159:1202	There was a significant difference between the 3 groups regarding the restoration of menstrual regularity (p = 0.02) that was obtained in all patients only in-group 3.					
30915778	6	59	theme	mass	1297:1300	arg1	reduction					1302:1310	the fat mass reduction	1289:1310	the fat mass reduction	1289:1310	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	2	60	theme	=	536:536	arg1	n					534:534	n = 21	534:539	n = 21	534:539	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	3	61	theme	body	771:774	arg1	circumference					800:812	waist circumference	794:812	waist circumference	794:812	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	61	theme	body	771:774	arg1	ratio					844:848	waist-hip ratio	834:848	waist-hip ratio (WHR)	834:854	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	61	theme	body	771:774	arg1	index					781:785	body mass index	771:785	body mass index (BMI)	771:791	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	61	theme	body	771:774	arg1	BMI					788:790	BMI	788:790	BMI	788:790	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	61	theme	body	771:774	arg1	circumference					819:831	hip circumference	815:831	hip circumference	815:831	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	3	61	theme	body	771:774	arg1	score					764:768	Ferriman-Gallwey score	747:768	Ferriman-Gallwey score	747:768	Menstrual cycle, Ferriman-Gallwey score, body mass index (BMI), waist circumference, hip circumference, waist-hip ratio (WHR), and body composition by bioimpedentiometry were measured at baseline, 3 and 6 months.					
30915778	2	62	dep	diet	519:522	arg1	myo-inositol					552:563	myo-inositol	552:563	myo-inositol (MI)	552:568	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	62	dep	diet	519:522	arg1	diet					619:622	diet	619:622	only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13)	514:727	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	62	dep	diet	519:522	arg1	MI					566:567	MI	566:567	MI	566:567	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	62	dep	diet	519:522	arg1	µg					591:592	folic acid 400 µg	576:592	folic acid 400 µg	576:592	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	62	dep	diet	519:522	arg1	diet					543:546	diet	543:546	diet	543:546	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	2	62	dep	diet	519:522	arg1	group					601:605	group 2	601:607	group 2	601:607	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	4	63	dep	weight	956:961	arg1	circumferences					983:996	circumferences	983:996	circumferences	983:996	RESULTS Body weight, BMI, waist and hip circumferences decreased significantly in all groups.					
30915778	2	64	theme	DCI	672:674	arg1	mg					682:683	D-chiroinositol (DCI) 27.6 mg	655:683	D-chiroinositol (DCI) 27.6 mg	655:683	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	1	65	theme	overweight/obese	215:230	arg1	women					237:241	overweight/obese PCOS women	215:241	overweight/obese PCOS women	215:241	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	6	66	from	MI+DCI	1217:1222	arg1	association					1227:1237	association	1227:1237	association with diet	1227:1247	CONCLUSIONS MI+DCI in association with diet seems to accelerate the weight loss and the fat mass reduction with a slight increase of percent lean mass, and this treatment contributes significantly in restoring the regularity of the menstrual cycle.					
30915778	5	67	theme	menstrual	1122:1130	arg1	regularity					1132:1141	menstrual regularity	1122:1141	menstrual regularity	1122:1141	There was a significant difference between the 3 groups regarding the restoration of menstrual regularity (p = 0.02) that was obtained in all patients only in-group 3.					
30915778	5	68	theme	significant	1049:1059	arg1	difference					1061:1070	a significant difference	1047:1070	a significant difference between the 3 groups regarding the restoration of menstrual regularity	1047:1141	There was a significant difference between the 3 groups regarding the restoration of menstrual regularity (p = 0.02) that was obtained in all patients only in-group 3.					
30915778	1	69	theme	PCOS	232:235	arg1	women					237:241	overweight/obese PCOS women	215:241	overweight/obese PCOS women	215:241	OBJECTIVE To evaluate, in overweight/obese PCOS women, which of three distinct treatment modalities achieved the greatest clinical benefits in terms of clinical and body composition outcomes.					
30915778	5	70	theme	regularity	1132:1141	arg1	restoration					1107:1117	the restoration	1103:1117	the restoration of menstrual regularity	1103:1141	There was a significant difference between the 3 groups regarding the restoration of menstrual regularity (p = 0.02) that was obtained in all patients only in-group 3.					
30915778	2	71	theme	acid	693:696	arg1	µg					702:703	folic acid 400 µg	687:703	folic acid 400 µg	687:703	PATIENTS AND METHODS Forty-three polycystic ovary syndrome (PCOS) overweight/obese patients were randomly treated for 6 months with: only diet (Group 1, n = 21); diet and myo-inositol (MI) 4 g + folic acid 400 µg daily (group 2, n = 10); diet in association with MI 1.1 g + D-chiroinositol (DCI) 27.6 mg + folic acid 400 µg daily (group 3, n = 13).					
30915778	0	72	from	clinical	109:116	arg1	women					151:155	women	151:155	women with polycystic ovary syndrome	151:186	Effects of three treatment modalities (diet, myoinositol or myoinositol associated with D-chiro-inositol) on clinical and body composition outcomes in women with polycystic ovary syndrome.					
31296384	5	0	contain	had	735:737	arg1	emulsion					726:733	the prepared emulsion	713:733	the prepared emulsion	713:733	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	5	0	contain	had	735:737	arg2	sizes					755:759	smaller droplet sizes	739:759	smaller droplet sizes	739:759	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	2	1	theme	molecular	355:363	arg1	weights					365:371	the molecular weights	351:371	the molecular weights of the polysaccharides	351:394	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	5	2	theme	smaller	739:745	arg1	sizes					755:759	smaller droplet sizes	739:759	smaller droplet sizes	739:759	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	6	3	theme	emulsion	942:949	arg1	destabilization					919:933	significant destabilization	907:933	significant destabilization of the emulsion	907:949	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	6	4	theme	ions	858:861	arg1	concentrations					827:840	low concentrations	823:840	low concentrations of Na+ and Ca2+ ions	823:861	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	7	5	theme	emulsifying	1083:1093	arg1	agent					1095:1099	a natural polysaccharide emulsifying agent	1058:1099	a natural polysaccharide emulsifying agent	1058:1099	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	4	6	from	variations	557:566	arg1	concentrations					589:602	cation ion concentrations	578:602	cation ion concentrations	578:602	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	4	6	from	variations	557:566	arg1	pH					571:572	pH	571:572	pH	571:572	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	6	7	theme	Ca2+	853:856	arg1	ions					858:861	Na+ and Ca2+ ions	845:861	Na+ and Ca2+ ions	845:861	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	3	8	theme	highest	513:519	arg1	capacity					533:540	the highest emulsifying capacity	509:540	the highest emulsifying capacity	509:540	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	2	9	dep	xylose	307:312	arg1	mannose					315:321	mannose	315:321	mannose	315:321	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	9	dep	xylose	307:312	arg1	glucose					338:344	glucose	338:344	glucose	338:344	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	9	dep	xylose	307:312	arg1	galactose					324:332	galactose	324:332	galactose	324:332	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	7	10	theme	natural	1060:1066	arg1	polysaccharide					1068:1081	a natural polysaccharide	1058:1081	a natural polysaccharide emulsifying agent	1058:1099	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	6	11	theme	Na+	845:847	arg1	ions					858:861	Na+ and Ca2+ ions	845:861	Na+ and Ca2+ ions	845:861	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	6	12	theme	significant	907:917	arg1	destabilization					919:933	significant destabilization	907:933	significant destabilization of the emulsion	907:949	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	1	13	theme	thinned-young	100:112	arg1	polysaccharides					120:134	The thinned-young apple polysaccharides	96:134	The thinned-young apple polysaccharides from three varieties	96:155	The thinned-young apple polysaccharides from three varieties were obtained by hot water extraction at 88 ̊C for 120 min.					
31296384	4	14	from	effect	627:632	arg1	properties					653:662	the emulsifying properties	637:662	the emulsifying properties of the extracted polysaccharides	637:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	4	15	theme	significant	615:625	arg1	effect					627:632	a significant effect	613:632	a significant effect on the emulsifying properties of the extracted polysaccharides	613:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	5	16	theme	higher	769:774	arg1	values					779:784	higher pH values	769:784	higher pH values	769:784	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	1	17	theme	apple	114:118	arg1	polysaccharides					120:134	The thinned-young apple polysaccharides	96:134	The thinned-young apple polysaccharides from three varieties	96:155	The thinned-young apple polysaccharides from three varieties were obtained by hot water extraction at 88 ̊C for 120 min.					
31296384	2	18	theme	polysaccharides	380:394	arg1	weights					365:371	the molecular weights	351:371	the molecular weights of the polysaccharides	351:394	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	0	19	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	1	20	from	varieties	147:155	arg1	polysaccharides					120:134	The thinned-young apple polysaccharides	96:134	The thinned-young apple polysaccharides from three varieties	96:155	The thinned-young apple polysaccharides from three varieties were obtained by hot water extraction at 88 ̊C for 120 min.					
31296384	0	21	theme	emulsifying	32:42	arg1	properties					44:53	emulsifying properties	32:53	emulsifying properties	32:53	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	5	22	theme	pH	776:777	arg1	values					779:784	higher pH values	769:784	higher pH values	769:784	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	7	23	theme	apple	1034:1038	arg1	polysaccharide					1040:1053	thinned-young apple polysaccharide	1020:1053	thinned-young apple polysaccharide	1020:1053	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	3	24	theme	emulsifying	521:531	arg1	capacity					533:540	the highest emulsifying capacity	509:540	the highest emulsifying capacity	509:540	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	2	25	theme	200-300 kDa	417:427	arg1	range					408:412	the range	404:412	the range of 200-300 kDa	404:427	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	7	26	theme	polysaccharide	1040:1053	arg1	application					1005:1015	the potential application	991:1015	the potential application of thinned-young apple polysaccharide	991:1053	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	6	27	theme	high	864:867	arg1	concentrations					869:882	high concentrations	864:882	high concentrations of Na+ and Ca2+	864:898	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	1	28	theme	hot	174:176	arg1	extraction					184:193	hot water extraction	174:193	hot water extraction	174:193	The thinned-young apple polysaccharides from three varieties were obtained by hot water extraction at 88 ̊C for 120 min.					
31296384	5	29	theme	droplet	747:753	arg1	sizes					755:759	smaller droplet sizes	739:759	smaller droplet sizes	739:759	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	6	30	theme	low	823:825	arg1	concentrations					827:840	low concentrations	823:840	low concentrations of Na+ and Ca2+ ions	823:861	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	2	31	theme	polysaccharides	264:278	arg1	xylose					307:312	xylose	307:312	xylose	307:312	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	31	theme	polysaccharides	264:278	arg1	same					301:304	same	301:304	same	301:304	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	31	theme	polysaccharides	264:278	arg1	monosaccharides					235:249	The compositional monosaccharides	217:249	The compositional monosaccharides of the three polysaccharides	217:278	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	3	32	theme	Qinyang	445:451	arg1	"					452:452	"Qinyang"	444:452	"Qinyang"	444:452	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	3	33	contain	had	505:507	arg2	capacity					533:540	the highest emulsifying capacity	509:540	the highest emulsifying capacity	509:540	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	3	33	contain	had	505:507	arg1	polysaccharide					474:487	the polysaccharide	470:487	the polysaccharide from "Jinshiji"	470:503	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	7	34	theme	thinned-young	1020:1032	arg1	polysaccharide					1040:1053	thinned-young apple polysaccharide	1020:1053	thinned-young apple polysaccharide	1020:1053	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	6	35	from	concentrations	827:840	arg1	emulsion					800:807	the emulsion	796:807	the emulsion	796:807	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	6	35	from	concentrations	827:840	arg1	stable					813:818	stable	813:818	stable	813:818	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	0	36	theme	apples	72:77	arg1	polysaccharides					79:93	thinned-young apples polysaccharides	58:93	thinned-young apples polysaccharides	58:93	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	6	37	theme	Ca2+	895:898	arg1	concentrations					869:882	high concentrations	864:882	high concentrations of Na+ and Ca2+	864:898	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	7	38	theme	potential	995:1003	arg1	application					1005:1015	the potential application	991:1015	the potential application of thinned-young apple polysaccharide	991:1053	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	4	39	contain	had	604:606	arg1	variations					557:566	the variations	553:566	the variations in pH and cation ion concentrations	553:602	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	4	39	contain	had	604:606	arg2	effect					627:632	a significant effect	613:632	a significant effect on the emulsifying properties of the extracted polysaccharides	613:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	4	40	theme	polysaccharides	681:695	arg1	properties					653:662	the emulsifying properties	637:662	the emulsifying properties of the extracted polysaccharides	637:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	0	41	theme	thinned-young	58:70	arg1	polysaccharides					79:93	thinned-young apples polysaccharides	58:93	thinned-young apples polysaccharides	58:93	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	6	42	theme	Na+	887:889	arg1	concentrations					869:882	high concentrations	864:882	high concentrations of Na+ and Ca2+	864:898	Although the emulsion was stable at low concentrations of Na+ and Ca2+ ions, high concentrations of Na+ and Ca2+ led to significant destabilization of the emulsion.					
31296384	7	43	theme	polysaccharide	1068:1081	arg1	agent					1095:1099	a natural polysaccharide emulsifying agent	1058:1099	a natural polysaccharide emulsifying agent	1058:1099	Conclusively, our results demonstrated the potential application of thinned-young apple polysaccharide as a natural polysaccharide emulsifying agent.					
31296384	4	44	theme	extracted	671:679	arg1	polysaccharides					681:695	the extracted polysaccharides	667:695	the extracted polysaccharides	667:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	2	45	theme	compositional	221:233	arg1	xylose					307:312	xylose	307:312	xylose	307:312	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	45	theme	compositional	221:233	arg1	same					301:304	same	301:304	same	301:304	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	45	theme	compositional	221:233	arg1	monosaccharides					235:249	The compositional monosaccharides	217:249	The compositional monosaccharides of the three polysaccharides	217:278	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	4	46	theme	emulsifying	641:651	arg1	properties					653:662	the emulsifying properties	637:662	the emulsifying properties of the extracted polysaccharides	637:695	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	3	47	theme	Pinklady	459:466	arg1	"					467:467	"Pinklady"	458:467	"Pinklady"	458:467	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	1	48	theme	water	178:182	arg1	extraction					184:193	hot water extraction	174:193	hot water extraction	174:193	The thinned-young apple polysaccharides from three varieties were obtained by hot water extraction at 88 ̊C for 120 min.					
31296384	5	49	theme	prepared	717:724	arg1	emulsion					726:733	the prepared emulsion	713:733	the prepared emulsion	713:733	At pH 2.0-4.0, the prepared emulsion had smaller droplet sizes than at higher pH values.					
31296384	2	50	dep	same	301:304	arg1	xylose					307:312	xylose	307:312	xylose	307:312	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	50	dep	same	301:304	arg1	same					301:304	same	301:304	same	301:304	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	2	50	dep	same	301:304	arg1	monosaccharides					235:249	The compositional monosaccharides	217:249	The compositional monosaccharides of the three polysaccharides	217:278	The compositional monosaccharides of the three polysaccharides were shown to be the same (xylose, mannose, galactose and glucose) and the molecular weights of the polysaccharides were in the range of 200-300 kDa.					
31296384	4	51	theme	ion	585:587	arg1	concentrations					589:602	cation ion concentrations	578:602	cation ion concentrations	578:602	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31296384	0	52	theme	polysaccharides	79:93	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	0	52	theme	polysaccharides	79:93	arg1	properties					44:53	emulsifying properties	32:53	emulsifying properties	32:53	Structural characterization and emulsifying properties of thinned-young apples polysaccharides.					
31296384	3	53	theme	Jinshiji	495:502	arg1	"					503:503	"Jinshiji"	494:503	"Jinshiji"	494:503	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	3	54	from	"	503:503	arg1	polysaccharide					474:487	the polysaccharide	470:487	the polysaccharide from "Jinshiji"	470:503	Compared with "Qinyang" and "Pinklady", the polysaccharide from "Jinshiji" had the highest emulsifying capacity.					
31296384	4	55	theme	cation	578:583	arg1	concentrations					589:602	cation ion concentrations	578:602	cation ion concentrations	578:602	Moreover, the variations in pH and cation ion concentrations had also a significant effect on the emulsifying properties of the extracted polysaccharides.					
31733246	6	0	dep	Fourier	905:911	arg1	transform					913:921	transform	913:921	transform infrared (FT-IR) spectrum	913:947	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	5	1	theme	molar	820:824	arg1	different					860:868	different	860:868	different	860:868	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	1	theme	molar	820:824	arg1	ratio					826:830	the molar ratio	816:830	the molar ratio of monosaccharide	816:848	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	2	2	theme	chloride	461:468	arg1	CSFF					480:483	CSFF	480:483	CSFF	480:483	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	2	theme	chloride	461:468	arg1	solution					470:477	calcium chloride solution	453:477	calcium chloride solution (CSFF)	453:484	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	7	3	theme	red	1039:1041	arg1	test					1043:1046	Congo red test	1033:1046	Congo red test	1033:1046	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	2	4	theme	hot	367:369	arg1	water					371:375	hot water	367:375	hot water (prepared fucoidan was named as WSFF)	367:413	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	7	5	from	differences	1108:1118	arg1	conformation					1132:1143	solution conformation	1123:1143	solution conformation of these samples	1123:1160	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	0	6	theme	fusiforme	117:125	arg1	fucoidans					127:135	Sargassum fusiforme fucoidans	107:135	Sargassum fusiforme fucoidans	107:135	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	2	7	dep	water	371:375	arg1	named					400:404	named	400:404	was named as WSFF	396:412	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	0	8	theme	Sargassum	107:115	arg1	fucoidans					127:135	Sargassum fusiforme fucoidans	107:135	Sargassum fusiforme fucoidans	107:135	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	9	9	theme	antioxidant	1457:1467	arg1	activities					1469:1478	antioxidant activities	1457:1478	antioxidant activities	1457:1478	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	4	10	theme	minimum	721:727	arg1	yield					729:733	the minimum yield	717:733	the minimum yield (3.94%)	717:741	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	4	10	theme	minimum	721:727	arg1	%					740:740	3.94%	736:740	3.94%	736:740	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	7	11	theme	Circular	1052:1059	arg1	spectroscopy					1071:1082	Circular dichroism spectroscopy	1052:1082	Circular dichroism spectroscopy	1052:1082	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	3	12	theme	S.	521:522	arg1	fucoidans					534:542	S. fusiforme fucoidans	521:542	S. fusiforme fucoidans	521:542	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	2	13	theme	extraction	337:346	arg1	water					371:375	hot water	367:375	hot water (prepared fucoidan was named as WSFF)	367:413	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	13	theme	extraction	337:346	arg1	methods					348:354	different extraction methods	327:354	different extraction methods	327:354	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	13	theme	extraction	337:346	arg1	acid					436:439	dilute hydrochloric acid	416:439	dilute hydrochloric acid (ASFF)	416:446	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	13	theme	extraction	337:346	arg1	solution					470:477	calcium chloride solution	453:477	calcium chloride solution (CSFF)	453:484	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	9	14	theme	conformation	1430:1441	arg1	behaviors					1443:1451	conformation behaviors	1430:1451	conformation behaviors	1430:1451	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	5	15	theme	ASFF	784:787	arg1	composition					763:773	The monosaccharide composition	744:773	The monosaccharide composition of WSFF, ASFF, and CSFF	744:797	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	15	theme	ASFF	784:787	arg1	similar					803:809	similar	803:809	similar	803:809	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	16	theme	monosaccharide	748:761	arg1	composition					763:773	The monosaccharide composition	744:773	The monosaccharide composition of WSFF, ASFF, and CSFF	744:797	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	16	theme	monosaccharide	748:761	arg1	similar					803:809	similar	803:809	similar	803:809	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	1	17	theme	active	191:196	arg1	component					198:206	an active component	188:206	an active component found in the cell wall of brown seaweeds	188:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	0	18	theme	fucoidans	127:135	arg1	activities					93:102	antioxidant activities	81:102	antioxidant activities	81:102	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	0	18	theme	fucoidans	127:135	arg1	properties					66:75	distinct physicochemical properties	41:75	distinct physicochemical properties	41:75	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	2	19	theme	calcium	453:459	arg1	CSFF					480:483	CSFF	480:483	CSFF	480:483	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	19	theme	calcium	453:459	arg1	solution					470:477	calcium chloride solution	453:477	calcium chloride solution (CSFF)	453:484	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	8	20	theme	hydroxyl	1267:1274	arg1	radical					1276:1282	hydroxyl radical	1267:1282	hydroxyl radical	1267:1282	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	9	21	theme	extraction	1371:1380	arg1	methods					1382:1388	extraction methods	1371:1388	extraction methods	1371:1388	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	0	22	theme	extraction	10:19	arg1	methods					21:27	Different extraction methods	0:27	Different extraction methods	0:27	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	7	23	theme	samples	1154:1160	arg1	conformation					1132:1143	solution conformation	1123:1143	solution conformation of these samples	1123:1160	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	2	24	theme	different	327:335	arg1	water					371:375	hot water	367:375	hot water (prepared fucoidan was named as WSFF)	367:413	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	24	theme	different	327:335	arg1	methods					348:354	different extraction methods	327:354	different extraction methods	327:354	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	24	theme	different	327:335	arg1	acid					436:439	dilute hydrochloric acid	416:439	dilute hydrochloric acid (ASFF)	416:446	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	24	theme	different	327:335	arg1	solution					470:477	calcium chloride solution	453:477	calcium chloride solution (CSFF)	453:484	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	9	25	theme	methods	1382:1388	arg1	influences					1357:1366	the influences	1353:1366	the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans	1353:1504	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	0	26	theme	Different	0:8	arg1	methods					21:27	Different extraction methods	0:27	Different extraction methods	0:27	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	5	27	theme	CSFF	794:797	arg1	composition					763:773	The monosaccharide composition	744:773	The monosaccharide composition of WSFF, ASFF, and CSFF	744:797	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	27	theme	CSFF	794:797	arg1	similar					803:809	similar	803:809	similar	803:809	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	8	28	theme	scavenging	1284:1293	arg1	assays					1295:1300	scavenging assays	1284:1300	scavenging assays	1284:1300	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	6	29	theme	acid	977:980	arg1	content					982:988	uronic acid content	970:988	uronic acid content	970:988	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	7	30	theme	spectroscopy	1071:1082	arg1	analysis					1084:1091	The Congo red test and Circular dichroism spectroscopy analysis	1029:1091	The Congo red test and Circular dichroism spectroscopy analysis	1029:1091	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	3	31	theme	molecular	581:589	arg1	conformations					591:603	molecular conformations	581:603	molecular conformations	581:603	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	8	32	theme	in	1213:1214	arg1	activities					1234:1243	distinct in vitro antioxidant activities	1204:1243	distinct in vitro antioxidant activities	1204:1243	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	3	33	theme	fusiforme	524:532	arg1	fucoidans					534:542	S. fusiforme fucoidans	521:542	S. fusiforme fucoidans	521:542	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	8	34	theme	antioxidant	1222:1232	arg1	activities					1234:1243	distinct in vitro antioxidant activities	1204:1243	distinct in vitro antioxidant activities	1204:1243	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	2	35	theme	dilute	416:421	arg1	ASFF					442:445	ASFF	442:445	ASFF	442:445	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	35	theme	dilute	416:421	arg1	acid					436:439	dilute hydrochloric acid	416:439	dilute hydrochloric acid (ASFF)	416:446	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	36	theme	hydrochloric	423:434	arg1	ASFF					442:445	ASFF	442:445	ASFF	442:445	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	36	theme	hydrochloric	423:434	arg1	acid					436:439	dilute hydrochloric acid	416:439	dilute hydrochloric acid (ASFF)	416:446	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	6	37	theme	molecular	887:895	arg1	weight					897:902	their molecular weight	881:902	their molecular weight	881:902	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	2	38	theme	prepared	378:385	arg1	fucoidan					387:394	prepared fucoidan	378:394	prepared fucoidan	378:394	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	2	38	theme	prepared	378:385	arg1	WSFF					409:412	WSFF	409:412	WSFF	409:412	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	3	39	theme	antioxidant	619:629	arg1	activities					631:640	in vitro antioxidant activities	610:640	in vitro antioxidant activities	610:640	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	2	40	theme	Sargassum	301:309	arg1	fusiforme					311:319	Sargassum fusiforme	301:319	Sargassum fusiforme	301:319	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	9	41	theme	fusiforme	1486:1494	arg1	fucoidans					1496:1504	S. fusiforme fucoidans	1483:1504	S. fusiforme fucoidans	1483:1504	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	1	42	theme	cell	221:224	arg1	wall					226:229	the cell wall	217:229	the cell wall of brown seaweeds	217:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	0	43	theme	distinct	41:48	arg1	properties					66:75	distinct physicochemical properties	41:75	distinct physicochemical properties	41:75	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	9	44	from	evidence	1341:1348	arg1	influences					1357:1366	the influences	1353:1366	the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans	1353:1504	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	45	from	influences	1357:1366	arg1	characteristics					1413:1427	the physicochemical characteristics	1393:1427	the physicochemical characteristics	1393:1427	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	45	from	influences	1357:1366	arg1	behaviors					1443:1451	conformation behaviors	1430:1451	conformation behaviors	1430:1451	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	45	from	influences	1357:1366	arg1	activities					1469:1478	antioxidant activities	1457:1478	antioxidant activities	1457:1478	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	46	theme	S.	1483:1484	arg1	fucoidans					1496:1504	S. fusiforme fucoidans	1483:1504	S. fusiforme fucoidans	1483:1504	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	4	47	theme	extraction	667:676	arg1	yield					678:682	the maximum extraction yield	655:682	the maximum extraction yield (11.24%)	655:691	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	4	47	theme	extraction	667:676	arg1	%					690:690	11.24%	685:690	11.24%	685:690	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	7	48	theme	dichroism	1061:1069	arg1	spectroscopy					1071:1082	Circular dichroism spectroscopy	1052:1082	Circular dichroism spectroscopy	1052:1082	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	3	49	theme	in	610:611	arg1	activities					631:640	in vitro antioxidant activities	610:640	in vitro antioxidant activities	610:640	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	6	50	theme	surface	950:956	arg1	morphology					958:967	surface morphology	950:967	surface morphology	950:967	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	7	51	theme	test	1043:1046	arg1	analysis					1084:1091	The Congo red test and Circular dichroism spectroscopy analysis	1029:1091	The Congo red test and Circular dichroism spectroscopy analysis	1029:1091	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	7	52	theme	solution	1123:1130	arg1	conformation					1132:1143	solution conformation	1123:1143	solution conformation of these samples	1123:1160	The Congo red test and Circular dichroism spectroscopy analysis displayed some differences in solution conformation of these samples.					
31733246	1	53	located	found	208:212	arg1	wall					226:229	the cell wall	217:229	the cell wall of brown seaweeds	217:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	1	53	located	found	208:212	arg2	component					198:206	an active component	188:206	an active component found in the cell wall of brown seaweeds	188:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	8	54	theme	distinct	1204:1211	arg1	activities					1234:1243	distinct in vitro antioxidant activities	1204:1243	distinct in vitro antioxidant activities	1204:1243	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	0	55	theme	physicochemical	50:64	arg1	properties					66:75	distinct physicochemical properties	41:75	distinct physicochemical properties	41:75	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	6	56	theme	sulfation	1004:1012	arg1	content					982:988	uronic acid content	970:988	uronic acid content	970:988	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	6	56	theme	sulfation	1004:1012	arg1	Fourier					905:911	Fourier	905:911	Fourier transform infrared (FT-IR) spectrum	905:947	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	6	56	theme	sulfation	1004:1012	arg1	weight					897:902	their molecular weight	881:902	their molecular weight	881:902	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	6	56	theme	sulfation	1004:1012	arg1	morphology					958:967	surface morphology	950:967	surface morphology	950:967	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	6	56	theme	sulfation	1004:1012	arg1	degree					994:999	degree	994:999	degree of sulfation	994:1012	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	9	57	theme	fucoidans	1496:1504	arg1	characteristics					1413:1427	the physicochemical characteristics	1393:1427	the physicochemical characteristics	1393:1427	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	57	theme	fucoidans	1496:1504	arg1	behaviors					1443:1451	conformation behaviors	1430:1451	conformation behaviors	1430:1451	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	9	57	theme	fucoidans	1496:1504	arg1	activities					1469:1478	antioxidant activities	1457:1478	antioxidant activities	1457:1478	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	3	58	theme	chemical	559:566	arg1	composition					568:578	their chemical composition	553:578	their chemical composition	553:578	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	9	59	theme	physicochemical	1397:1411	arg1	characteristics					1413:1427	the physicochemical characteristics	1393:1427	the physicochemical characteristics	1393:1427	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	5	60	theme	monosaccharide	835:848	arg1	different					860:868	different	860:868	different	860:868	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	60	theme	monosaccharide	835:848	arg1	ratio					826:830	the molar ratio	816:830	the molar ratio of monosaccharide	816:848	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	8	61	dep	showed	1197:1202	arg1	evaluated					1245:1253	evaluated	1245:1253	showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays	1197:1300	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	4	62	theme	maximum	659:665	arg1	yield					678:682	the maximum extraction yield	655:682	the maximum extraction yield (11.24%)	655:691	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	4	62	theme	maximum	659:665	arg1	%					690:690	11.24%	685:690	11.24%	685:690	ASFF showed the maximum extraction yield (11.24%), whereas CSFF exhibited the minimum yield (3.94%).					
31733246	9	63	theme	present	1307:1313	arg1	study					1315:1319	The present study	1303:1319	The present study	1303:1319	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	1	64	theme	brown	234:238	arg1	seaweeds					240:247	brown seaweeds	234:247	brown seaweeds	234:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	8	65	dep	in	1213:1214	arg1	vitro					1216:1220	vitro	1216:1220	vitro	1216:1220	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	1	66	theme	complex	152:158	arg1	polysaccharide					169:182	a complex sulfated polysaccharide	150:182	a complex sulfated polysaccharide	150:182	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	1	66	theme	complex	152:158	arg1	Fucoidan					138:145	Fucoidan	138:145	Fucoidan	138:145	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	1	67	theme	seaweeds	240:247	arg1	wall					226:229	the cell wall	217:229	the cell wall of brown seaweeds	217:247	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	0	68	theme	antioxidant	81:91	arg1	activities					93:102	antioxidant activities	81:102	antioxidant activities	81:102	Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans.					
31733246	8	69	dep	DPPH	1258:1261	arg1	assays					1295:1300	scavenging assays	1284:1300	scavenging assays	1284:1300	Furthermore, WSFF, ASFF, and CSFF showed distinct in vitro antioxidant activities evaluated by DPPH and hydroxyl radical scavenging assays.					
31733246	2	70	theme	present	257:263	arg1	study					265:269	the present study	253:269	the present study	253:269	In the present study, fucoidans were obtained from Sargassum fusiforme using different extraction methods, including hot water (prepared fucoidan was named as WSFF), dilute hydrochloric acid (ASFF), and calcium chloride solution (CSFF).					
31733246	6	71	theme	uronic	970:975	arg1	content					982:988	uronic acid content	970:988	uronic acid content	970:988	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	1	72	theme	sulfated	160:167	arg1	polysaccharide					169:182	a complex sulfated polysaccharide	150:182	a complex sulfated polysaccharide	150:182	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	1	72	theme	sulfated	160:167	arg1	Fucoidan					138:145	Fucoidan	138:145	Fucoidan	138:145	Fucoidan is a complex sulfated polysaccharide and an active component found in the cell wall of brown seaweeds.					
31733246	6	73	dep	transform	913:921	arg1	infrared					923:930	infrared	923:930	transform infrared (FT-IR) spectrum	913:947	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31733246	5	74	theme	WSFF	778:781	arg1	composition					763:773	The monosaccharide composition	744:773	The monosaccharide composition of WSFF, ASFF, and CSFF	744:797	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	5	74	theme	WSFF	778:781	arg1	similar					803:809	similar	803:809	similar	803:809	The monosaccharide composition of WSFF, ASFF, and CSFF was similar, but the molar ratio of monosaccharide was quite different.					
31733246	3	75	dep	in	610:611	arg1	vitro					613:617	vitro	613:617	vitro	613:617	The assessments were performed on S. fusiforme fucoidans based on their chemical composition, molecular conformations, and in vitro antioxidant activities.					
31733246	9	76	theme	scientific	1330:1339	arg1	evidence					1341:1348	scientific evidence	1330:1348	scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans	1330:1504	The present study provides scientific evidence on the influences of extraction methods on the physicochemical characteristics, conformation behaviors and antioxidant activities of S. fusiforme fucoidans.					
31733246	6	77	theme	FT-IR	933:937	arg1	spectrum					940:947	(FT-IR) spectrum	932:947	(FT-IR) spectrum	932:947	Moreover, their molecular weight, Fourier transform infrared (FT-IR) spectrum, surface morphology, uronic acid content and degree of sulfation were distinct.					
31086063	4	0	theme	%	676:676	arg1	concentration					658:670	a concentration	656:670	a concentration of 4%	656:676	The results showed that hemicellulose at a concentration of 4% increased the yield of the mycelia biomass to twice that of the control group.					
31086063	7	1	theme	Monosaccharide	1001:1014	arg1	analysis					1028:1035	Monosaccharide composition analysis	1001:1035	Monosaccharide composition analysis	1001:1035	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31086063	2	2	theme	mycelia	349:355	arg1	growth					339:344	the growth	335:344	the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	335:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	3	from	effect	297:302	arg1	growth					339:344	the growth	335:344	the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	335:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	6	4	theme	polysaccharide	959:972	arg1	biosynthesis					974:985	polysaccharide biosynthesis	959:985	polysaccharide biosynthesis	959:985	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	0	5	from	Effect	4:9	arg1	Properties					42:51	Properties	42:51	Properties of Polysaccharides	42:70	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus edodes and Their Antioxidant Evaluation.					
31086063	6	6	from	biosynthesis	974:985	arg1	edodes					993:998	edodes	993:998	edodes	993:998	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	3	7	theme	LEPs	498:501	arg1	properties					484:493	The antioxidant properties	468:493	The antioxidant properties of LEPs	468:501	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	2	8	from	mycelia	434:440	arg1	mycelia					349:355	mycelia	349:355	mycelia	349:355	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	8	from	mycelia	434:440	arg1	polysaccharides					403:417	polysaccharides	403:417	polysaccharides from L. edodes mycelia (LEPs)	403:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	8	from	mycelia	434:440	arg1	properties					389:398	the physicochemical properties	369:398	the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	369:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	3	9	theme	scavenging	534:543	arg1	assays					545:550	radical scavenging assays	526:550	radical scavenging assays in vitro	526:559	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	9	10	from	effect	1468:1473	arg1	elegans					1481:1487	C. elegans	1478:1487	C. elegans	1478:1487	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	9	11	theme	Antioxidant	1293:1303	arg1	assays					1305:1310	Antioxidant assays	1293:1310	Antioxidant assays	1293:1310	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	10	12	theme	hemicellulose	1574:1586	arg1	addition					1562:1569	the addition	1558:1569	the addition of hemicellulose and lignin	1558:1597	In summary, the addition of hemicellulose and lignin improved the biosynthesis and bioactivity of LEPs.					
31086063	1	13	contain	possess	164:170	arg1	polysaccharides					148:162	polysaccharides	148:162	polysaccharides	148:162	Lentinus edodes, whose polysaccharides possess diverse bioactivities, commonly grows on hardwood sawdust composed of hemicellulose, lignin and cellulose.					
31086063	1	13	contain	possess	164:170	arg2	bioactivities					180:192	diverse bioactivities	172:192	diverse bioactivities	172:192	Lentinus edodes, whose polysaccharides possess diverse bioactivities, commonly grows on hardwood sawdust composed of hemicellulose, lignin and cellulose.					
31086063	6	14	theme	mycelia	940:946	arg1	growth					948:953	mycelia growth	940:953	mycelia growth	940:953	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	0	15	theme	Antioxidant	101:111	arg1	Evaluation					113:122	and Their Antioxidant Evaluation	91:122	Evaluation	113:122	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus edodes and Their Antioxidant Evaluation.					
31086063	6	16	from	growth	948:953	arg1	edodes					993:998	edodes	993:998	edodes	993:998	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	4	17	from	concentration	658:670	arg1	hemicellulose					639:651	hemicellulose	639:651	hemicellulose at a concentration of 4%	639:676	The results showed that hemicellulose at a concentration of 4% increased the yield of the mycelia biomass to twice that of the control group.					
31086063	1	18	theme	diverse	172:178	arg1	bioactivities					180:192	diverse bioactivities	172:192	diverse bioactivities	172:192	Lentinus edodes, whose polysaccharides possess diverse bioactivities, commonly grows on hardwood sawdust composed of hemicellulose, lignin and cellulose.					
31086063	9	19	theme	thermal	1449:1455	arg1	effect					1468:1473	a thermal resistance effect	1447:1473	a thermal resistance effect on C. elegans	1447:1487	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	7	20	theme	higher	1118:1123	arg1	concentrations					1125:1138	higher concentrations	1118:1138	higher concentrations of hemicellulose	1118:1155	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31086063	2	21	from	mycelia	349:355	arg1	mycelia					434:440	L. edodes mycelia	424:440	L. edodes mycelia (LEPs)	424:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	21	from	mycelia	349:355	arg1	LEPs					443:446	LEPs	443:446	LEPs	443:446	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	3	22	theme	radical	526:532	arg1	assays					545:550	radical scavenging assays	526:550	radical scavenging assays in vitro	526:559	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	10	23	dep	biosynthesis	1612:1623	arg1	the					1608:1610	the	1608:1610	the	1608:1610	In summary, the addition of hemicellulose and lignin improved the biosynthesis and bioactivity of LEPs.					
31086063	8	24	theme	molecular	1162:1170	arg1	structure					1172:1180	The molecular structure	1158:1180	The molecular structure	1158:1180	The molecular structure, including the molecular weight distribution and configuration type, was affected by hemicellulose and lignin.					
31086063	2	25	theme	hemicellulose	307:319	arg1	effect					297:302	the effect	293:302	the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	293:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	5	26	theme	mycelia	841:847	arg1	content					826:832	the polysaccharide content	807:832	the polysaccharide content of the mycelia	807:847	Meanwhile, when cultured with 4.0% hemicellulose, the polysaccharide content of the mycelia was raised by 112.2%.					
31086063	0	27	theme	Hemicellulose	14:26	arg1	Effect					4:9	The Effect	0:9	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus	0:82	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus edodes and Their Antioxidant Evaluation.					
31086063	8	28	dep	distribution	1214:1225	arg1	type					1245:1248	type	1245:1248	type	1245:1248	The molecular structure, including the molecular weight distribution and configuration type, was affected by hemicellulose and lignin.					
31086063	9	29	theme	resistance	1457:1466	arg1	effect					1468:1473	a thermal resistance effect	1447:1473	a thermal resistance effect on C. elegans	1447:1487	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	3	30	theme	in	552:553	arg1	assays					545:550	radical scavenging assays	526:550	radical scavenging assays in vitro	526:559	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	4	31	theme	biomass	713:719	arg1	mycelia					705:711	the mycelia	701:711	the mycelia biomass to twice that of the control group	701:754	The results showed that hemicellulose at a concentration of 4% increased the yield of the mycelia biomass to twice that of the control group.					
31086063	6	32	theme	lignin	917:922	arg1	concentration					900:912	the appropriate concentration	884:912	the appropriate concentration of lignin	884:922	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	7	33	theme	higher	1051:1056	arg1	content					1058:1064	a higher content	1049:1064	a higher content of xylose	1049:1074	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31086063	2	34	theme	polysaccharides	403:417	arg1	mycelia					349:355	mycelia	349:355	mycelia	349:355	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	34	theme	polysaccharides	403:417	arg1	properties					389:398	the physicochemical properties	369:398	the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	369:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	0	35	theme	Lignin	32:37	arg1	Effect					4:9	The Effect	0:9	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus	0:82	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus edodes and Their Antioxidant Evaluation.					
31086063	9	36	theme	antioxidant	1508:1518	arg1	effect					1520:1525	the antioxidant effect	1504:1525	the antioxidant effect	1504:1525	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	9	36	theme	antioxidant	1508:1518	arg1	potent					1530:1535	potent	1530:1535	potent	1530:1535	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	10	37	theme	lignin	1592:1597	arg1	addition					1562:1569	the addition	1558:1569	the addition of hemicellulose and lignin	1558:1597	In summary, the addition of hemicellulose and lignin improved the biosynthesis and bioactivity of LEPs.					
31086063	3	38	theme	elegans	592:598	arg1	model					600:604	the Caenorhabditis elegans model	573:604	the Caenorhabditis elegans model	573:604	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	1	39	theme	hardwood	213:220	arg1	sawdust					222:228	hardwood sawdust	213:228	hardwood sawdust composed of hemicellulose, lignin and cellulose	213:276	Lentinus edodes, whose polysaccharides possess diverse bioactivities, commonly grows on hardwood sawdust composed of hemicellulose, lignin and cellulose.					
31086063	2	40	theme	physicochemical	373:387	arg1	properties					389:398	the physicochemical properties	369:398	the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	369:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	5	41	theme	4.0	787:789	arg1	%					790:790	%	790:790	%	790:790	Meanwhile, when cultured with 4.0% hemicellulose, the polysaccharide content of the mycelia was raised by 112.2%.					
31086063	2	42	theme	properties	389:398	arg1	growth					339:344	the growth	335:344	the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	335:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	6	43	theme	appropriate	888:898	arg1	concentration					900:912	the appropriate concentration	884:912	the appropriate concentration of lignin	884:922	In addition, the appropriate concentration of lignin could stimulate mycelia growth and polysaccharide biosynthesis in L. edodes.					
31086063	7	44	theme	composition	1016:1026	arg1	analysis					1028:1035	Monosaccharide composition analysis	1001:1035	Monosaccharide composition analysis	1001:1035	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31086063	3	45	theme	antioxidant	472:482	arg1	properties					484:493	The antioxidant properties	468:493	The antioxidant properties of LEPs	468:501	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	8	46	theme	weight	1207:1212	arg1	distribution					1214:1225	the molecular weight distribution	1193:1225	the molecular weight distribution	1193:1225	The molecular structure, including the molecular weight distribution and configuration type, was affected by hemicellulose and lignin.					
31086063	9	47	theme	higher	1388:1393	arg1	abilities					1414:1422	higher radical scavenging abilities	1388:1422	higher radical scavenging abilities	1388:1422	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	3	48	theme	Caenorhabditis	577:590	arg1	model					600:604	the Caenorhabditis elegans model	573:604	the Caenorhabditis elegans model	573:604	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	8	49	theme	molecular	1197:1205	arg1	distribution					1214:1225	the molecular weight distribution	1193:1225	the molecular weight distribution	1193:1225	The molecular structure, including the molecular weight distribution and configuration type, was affected by hemicellulose and lignin.					
31086063	9	50	theme	radical	1395:1401	arg1	abilities					1414:1422	higher radical scavenging abilities	1388:1422	higher radical scavenging abilities	1388:1422	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	10	51	theme	LEPs	1644:1647	arg1	bioactivity					1629:1639	bioactivity	1629:1639	bioactivity	1629:1639	In summary, the addition of hemicellulose and lignin improved the biosynthesis and bioactivity of LEPs.					
31086063	10	51	theme	LEPs	1644:1647	arg1	biosynthesis					1612:1623	biosynthesis	1612:1623	biosynthesis	1612:1623	In summary, the addition of hemicellulose and lignin improved the biosynthesis and bioactivity of LEPs.					
31086063	7	52	theme	xylose	1069:1074	arg1	content					1058:1064	a higher content	1049:1064	a higher content of xylose	1049:1074	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31086063	1	53	theme	Lentinus	125:132	arg1	edodes					134:139	Lentinus edodes	125:139	Lentinus edodes	125:139	Lentinus edodes, whose polysaccharides possess diverse bioactivities, commonly grows on hardwood sawdust composed of hemicellulose, lignin and cellulose.					
31086063	9	54	theme	scavenging	1403:1412	arg1	abilities					1414:1422	higher radical scavenging abilities	1388:1422	higher radical scavenging abilities	1388:1422	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	4	55	theme	control	742:748	arg1	group					750:754	the control group	738:754	the control group	738:754	The results showed that hemicellulose at a concentration of 4% increased the yield of the mycelia biomass to twice that of the control group.					
31086063	2	56	theme	lignin	325:330	arg1	effect					297:302	the effect	293:302	the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs)	293:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	4	57	theme	mycelia	705:711	arg1	yield					692:696	the yield	688:696	the yield of the mycelia biomass to twice that of the control group	688:754	The results showed that hemicellulose at a concentration of 4% increased the yield of the mycelia biomass to twice that of the control group.					
31086063	0	58	theme	Polysaccharides	56:70	arg1	Properties					42:51	Properties	42:51	Properties of Polysaccharides	42:70	The Effect of Hemicellulose and Lignin on Properties of Polysaccharides in Lentinus edodes and Their Antioxidant Evaluation.					
31086063	5	59	theme	polysaccharide	811:824	arg1	content					826:832	the polysaccharide content	807:832	the polysaccharide content of the mycelia	807:847	Meanwhile, when cultured with 4.0% hemicellulose, the polysaccharide content of the mycelia was raised by 112.2%.					
31086063	2	60	from	properties	389:398	arg1	mycelia					434:440	L. edodes mycelia	424:440	L. edodes mycelia (LEPs)	424:447	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	2	60	from	properties	389:398	arg1	LEPs					443:446	LEPs	443:446	LEPs	443:446	In this study the effect of hemicellulose and lignin on the growth of mycelia, as well as the physicochemical properties of polysaccharides from L. edodes mycelia (LEPs) were investigated.					
31086063	3	61	dep	in	552:553	arg1	vitro					555:559	vitro	555:559	vitro	555:559	The antioxidant properties of LEPs were evaluated through radical scavenging assays in vitro and through the Caenorhabditis elegans model in vivo.					
31086063	5	62	theme	%	790:790	arg1	hemicellulose					792:804	4.0% hemicellulose	787:804	4.0% hemicellulose	787:804	Meanwhile, when cultured with 4.0% hemicellulose, the polysaccharide content of the mycelia was raised by 112.2%.					
31086063	9	63	contain	possessed	1378:1386	arg2	abilities					1414:1422	higher radical scavenging abilities	1388:1422	higher radical scavenging abilities	1388:1422	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	9	63	contain	possessed	1378:1386	arg1	LEPs					1327:1330	LEPs	1327:1330	LEPs supplemented with hemicellulose and/or lignin	1327:1376	Antioxidant assays indicated that LEPs supplemented with hemicellulose and/or lignin possessed higher radical scavenging abilities in vitro and exhibited a thermal resistance effect on C. elegans, implying that the antioxidant effect is potent in vivo.					
31086063	7	64	theme	hemicellulose	1143:1155	arg1	concentrations					1125:1138	higher concentrations	1118:1138	higher concentrations of hemicellulose	1118:1155	Monosaccharide composition analysis showed that a higher content of xylose was found when mycelia were cultured with higher concentrations of hemicellulose.					
31793753	6	0	theme	carbon	1088:1093	arg1	sources					1095:1101	more complex carbon sources	1075:1101	more complex carbon sources	1075:1101	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	1	1	theme	water	253:257	arg1	capacity					269:276	water retention capacity	253:276	water retention capacity	253:276	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	9	2	theme	biofilms	1617:1624	arg1	view					1609:1612	a novel view	1601:1612	a novel view of biofilms	1601:1624	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	2	theme	biofilms	1617:1624	arg1	biomaterials					1648:1659	potentially useful biomaterials	1629:1659	potentially useful biomaterials	1629:1659	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	6	3	theme	WRV	1177:1179	arg1	trend					1181:1185	the lower WRV trend	1167:1185	the lower WRV trend	1167:1185	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	9	4	theme	useful	1641:1646	arg1	view					1609:1612	a novel view	1601:1612	a novel view of biofilms	1601:1624	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	4	theme	useful	1641:1646	arg1	biomaterials					1648:1659	potentially useful biomaterials	1629:1659	potentially useful biomaterials	1629:1659	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	3	5	theme	water	545:549	arg1	values					561:566	decreased water retention values	535:566	decreased water retention values (WRV)	535:572	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	5	theme	water	545:549	arg1	WRV					569:571	WRV	569:571	WRV	569:571	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	5	6	theme	relative	855:862	arg1	quantity					864:871	relative quantity	855:871	relative quantity	855:871	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	9	7	used	used	1528:1531	arg2	source					1514:1519	carbon source	1507:1519	carbon source	1507:1519	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	7	used	used	1528:1531	arg2	lever					1544:1548	process lever	1536:1548	process lever	1536:1548	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	1	8	theme	process	184:190	arg1	complexity					168:177	carbon source complexity	154:177	carbon source complexity	154:177	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	1	8	theme	process	184:190	arg1	lever					192:196	a process lever	182:196	a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta	182:320	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	4	9	theme	laser	622:626	arg1	microscopy					637:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	0	10	theme	discreta	118:125	arg1	biofilms					127:134	Neurospora discreta biofilms	107:134	Neurospora discreta biofilms	107:134	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	5	11	theme	carbon	909:914	arg1	complexity					923:932	carbon source complexity	909:932	carbon source complexity	909:932	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	8	12	theme	biofilms	1439:1446	arg1	composition					1406:1416	composition	1406:1416	composition	1406:1416	This in turn impacts the microstructure, composition and water content of biofilms.					
31793753	8	12	theme	biofilms	1439:1446	arg1	content					1428:1434	water content	1422:1434	water content	1422:1434	This in turn impacts the microstructure, composition and water content of biofilms.					
31793753	8	12	theme	biofilms	1439:1446	arg1	microstructure					1390:1403	microstructure	1390:1403	microstructure	1390:1403	This in turn impacts the microstructure, composition and water content of biofilms.					
31793753	7	13	theme	cells	1358:1362	arg1	proportions					1335:1345	the relative proportions	1322:1345	the relative proportions of ECM and cells	1322:1362	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	9	14	theme	STUDY	1480:1484	arg1	IMPACT					1466:1471	IMPACT	1466:1471	IMPACT	1466:1471	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	14	theme	STUDY	1480:1484	arg1	SIGNIFICANCE					1449:1460	SIGNIFICANCE	1449:1460	SIGNIFICANCE	1449:1460	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	7	15	theme	ECM	1350:1352	arg1	proportions					1335:1345	the relative proportions	1322:1345	the relative proportions of ECM and cells	1322:1362	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	4	16	theme	stained	718:724	arg1	areas					726:730	relative stained areas	709:730	relative stained areas of cells and carbohydrates from fluorescence imaging of samples	709:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	6	17	theme	matrix	1032:1037	arg1	analysis					992:999	The chemical analysis	979:999	The chemical analysis of the extracted extracellular matrix (ECM)	979:1043	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	18	contain	had	1103:1105	arg2	content					1138:1144	lower carbohydrate and protein content	1107:1144	content	1138:1144	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	18	contain	had	1103:1105	arg2	carbohydrate					1113:1124	lower carbohydrate and protein content	1107:1144	carbohydrate	1113:1124	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	18	contain	had	1103:1105	arg1	biofilms					1057:1064	biofilms	1057:1064	biofilms grown on more complex carbon sources	1057:1101	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	0	19	theme	water	51:55	arg1	retention					57:65	water retention	51:65	water retention	51:65	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	3	20	theme	harvested	593:601	arg1	biofilms					603:610	harvested biofilms	593:610	harvested biofilms	593:610	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	6	21	theme	extracted	1008:1016	arg1	ECM					1040:1042	ECM	1040:1042	ECM	1040:1042	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	21	theme	extracted	1008:1016	arg1	matrix					1032:1037	the extracted extracellular matrix	1004:1037	the extracted extracellular matrix (ECM)	1004:1043	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	4	22	from	imaging	777:783	arg1	areas					726:730	relative stained areas	709:730	relative stained areas of cells and carbohydrates from fluorescence imaging of samples	709:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	1	23	theme	source	161:166	arg1	complexity					168:177	carbon source complexity	154:177	carbon source complexity	154:177	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	1	23	theme	source	161:166	arg1	lever					192:196	a process lever	182:196	a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta	182:320	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	0	24	from	Influence	0:8	arg1	composition					92:102	extracellular matrix composition	71:102	extracellular matrix composition	71:102	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	0	24	from	Influence	0:8	arg1	porosity					41:48	porosity	41:48	porosity	41:48	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	0	24	from	Influence	0:8	arg1	retention					57:65	water retention	51:65	water retention	51:65	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	0	25	theme	matrix	85:90	arg1	composition					92:102	extracellular matrix composition	71:102	extracellular matrix composition	71:102	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	6	26	theme	lower	1171:1175	arg1	trend					1181:1185	the lower WRV trend	1167:1185	the lower WRV trend	1167:1185	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	27	theme	chemical	983:990	arg1	analysis					992:999	The chemical analysis	979:999	The chemical analysis of the extracted extracellular matrix (ECM)	979:1043	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	7	28	theme	relative	1326:1333	arg1	proportions					1335:1345	the relative proportions	1322:1345	the relative proportions of ECM and cells	1322:1362	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	0	29	theme	carbon	13:18	arg1	complexity					27:36	carbon source complexity	13:36	carbon source complexity	13:36	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	5	30	theme	cells	876:880	arg1	Porosity					842:849	Porosity	842:849	Porosity	842:849	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	5	30	theme	cells	876:880	arg1	quantity					864:871	relative quantity	855:871	relative quantity	855:871	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	4	31	theme	blue	816:819	arg1	Trypan					809:814	Trypan blue	809:819	Trypan blue	809:819	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	0	32	theme	complexity	27:36	arg1	Influence					0:8	Influence	0:8	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.	0:135	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	1	33	theme	Neurospora	302:311	arg1	discreta					313:320	Neurospora discreta	302:320	Neurospora discreta	302:320	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	6	34	dep	had	1103:1105	arg1	explains					1158:1165	explains	1158:1165	explains	1158:1165	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	3	35	theme	carbon	486:491	arg1	source					493:498	carbon source	486:498	carbon source	486:498	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	9	36	theme	biofilms	1579:1586	arg1	properties					1565:1574	the properties	1561:1574	the properties of biofilms	1561:1586	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	7	37	theme	metabolic	1274:1282	arg1	pathway					1284:1290	the metabolic pathway	1270:1290	the metabolic pathway of cells	1270:1299	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	4	38	theme	samples	788:794	arg1	imaging					777:783	fluorescence imaging	764:783	fluorescence imaging of samples	764:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	2	39	theme	RESULTS	335:341	arg1	Biofilms					343:350	METHODS AND RESULTS Biofilms	323:350	METHODS AND RESULTS Biofilms	323:350	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	6	40	theme	lower	1107:1111	arg1	carbohydrate					1113:1124	lower carbohydrate and protein content	1107:1144	carbohydrate	1113:1124	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	8	41	theme	water	1422:1426	arg1	content					1428:1434	water content	1422:1434	water content	1422:1434	This in turn impacts the microstructure, composition and water content of biofilms.					
31793753	2	42	theme	fungus	383:388	arg1	discreta					393:400	nonpathogenic fungus N. discreta	369:400	nonpathogenic fungus N. discreta	369:400	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	1	43	theme	chemical	228:235	arg1	composition					237:247	chemical composition	228:247	chemical composition	228:247	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	9	44	theme	novel	1603:1607	arg1	view					1609:1612	a novel view	1601:1612	a novel view of biofilms	1601:1624	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	44	theme	novel	1603:1607	arg1	biomaterials					1648:1659	potentially useful biomaterials	1629:1659	potentially useful biomaterials	1629:1659	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	7	45	theme	carbon	1248:1253	arg1	source					1255:1260	carbon source	1248:1260	carbon source	1248:1260	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	6	46	theme	complex	1080:1086	arg1	sources					1095:1101	more complex carbon sources	1075:1101	more complex carbon sources	1075:1101	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	3	47	from	sucrose	505:511	arg1	increase					460:467	The increase	456:467	The increase in complexity of carbon source from sucrose to lignin	456:521	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	48	theme	decreased	535:543	arg1	values					561:566	decreased water retention values	535:566	decreased water retention values (WRV)	535:572	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	48	theme	decreased	535:543	arg1	WRV					569:571	WRV	569:571	WRV	569:571	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	1	49	theme	retention	259:267	arg1	capacity					269:276	water retention capacity	253:276	water retention capacity	253:276	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	9	50	theme	carbon	1507:1512	arg1	lever					1544:1548	process lever	1536:1548	process lever	1536:1548	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	50	theme	carbon	1507:1512	arg1	source					1514:1519	carbon source	1507:1519	carbon source	1507:1519	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	4	51	theme	Fluor	831:835	arg1	Alexa					825:829	Alexa Fluor 488	825:839	Alexa Fluor 488	825:839	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	2	52	theme	METHODS	323:329	arg1	Biofilms					343:350	METHODS AND RESULTS Biofilms	323:350	METHODS AND RESULTS Biofilms	323:350	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	0	53	theme	biofilms	127:134	arg1	composition					92:102	extracellular matrix composition	71:102	extracellular matrix composition	71:102	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	0	53	theme	biofilms	127:134	arg1	porosity					41:48	porosity	41:48	porosity	41:48	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	0	53	theme	biofilms	127:134	arg1	retention					57:65	water retention	51:65	water retention	51:65	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	3	54	theme	retention	551:559	arg1	values					561:566	decreased water retention values	535:566	decreased water retention values (WRV)	535:572	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	54	theme	retention	551:559	arg1	WRV					569:571	WRV	569:571	WRV	569:571	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	4	55	theme	Confocal	613:620	arg1	microscopy					637:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	3	56	theme	wet	578:580	arg1	weights					582:588	wet weights	578:588	wet weights of harvested biofilms	578:610	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	1	57	dep	microstructure	212:225	arg1	the					208:210	the	208:210	the	208:210	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	4	58	theme	scanning	628:635	arg1	microscopy					637:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	3	59	theme	biofilms	603:610	arg1	values					561:566	decreased water retention values	535:566	decreased water retention values (WRV)	535:572	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	59	theme	biofilms	603:610	arg1	weights					582:588	wet weights	578:588	wet weights of harvested biofilms	578:610	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	59	theme	biofilms	603:610	arg1	WRV					569:571	WRV	569:571	WRV	569:571	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	4	60	used	used	652:655	arg2	microscopy					637:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy	613:646	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	5	61	theme	source	916:921	arg1	complexity					923:932	carbon source complexity	909:932	carbon source complexity	909:932	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	4	62	theme	relative	709:716	arg1	areas					726:730	relative stained areas	709:730	relative stained areas of cells and carbohydrates from fluorescence imaging of samples	709:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	8	63	dep	microstructure	1390:1403	arg1	the					1386:1388	the	1386:1388	the	1386:1388	This in turn impacts the microstructure, composition and water content of biofilms.					
31793753	6	64	theme	extracellular	1018:1030	arg1	ECM					1040:1042	ECM	1040:1042	ECM	1040:1042	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	6	64	theme	extracellular	1018:1030	arg1	matrix					1032:1037	the extracted extracellular matrix	1004:1037	the extracted extracellular matrix (ECM)	1004:1043	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	0	65	theme	extracellular	71:83	arg1	composition					92:102	extracellular matrix composition	71:102	extracellular matrix composition	71:102	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	1	66	theme	carbon	154:159	arg1	complexity					168:177	carbon source complexity	154:177	carbon source complexity	154:177	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	1	66	theme	carbon	154:159	arg1	lever					192:196	a process lever	182:196	a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta	182:320	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	4	67	theme	bright-field	684:695	arg1	images					697:702	bright-field images	684:702	bright-field images	684:702	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	5	68	from	increase	897:904	arg1	complexity					923:932	carbon source complexity	909:932	carbon source complexity	909:932	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	5	69	theme	carbohydrates	954:966	arg1	amount					944:949	the amount	940:949	the amount of carbohydrates	940:966	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	5	69	theme	carbohydrates	954:966	arg1	carbohydrates					954:966	carbohydrates	954:966	carbohydrates	954:966	Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased.					
31793753	4	70	theme	carbohydrates	745:757	arg1	areas					726:730	relative stained areas	709:730	relative stained areas of cells and carbohydrates from fluorescence imaging of samples	709:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	0	71	theme	Neurospora	107:116	arg1	biofilms					127:134	Neurospora discreta biofilms	107:134	Neurospora discreta biofilms	107:134	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	4	72	theme	fluorescence	764:775	arg1	imaging					777:783	fluorescence imaging	764:783	fluorescence imaging of samples	764:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	9	73	theme	process	1536:1542	arg1	lever					1544:1548	process lever	1536:1548	process lever	1536:1548	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	9	73	theme	process	1536:1542	arg1	source					1514:1519	carbon source	1507:1519	carbon source	1507:1519	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	2	74	theme	carbon	441:446	arg1	source					448:453	carbon source	441:453	carbon source	441:453	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	2	75	theme	N.	390:391	arg1	discreta					393:400	nonpathogenic fungus N. discreta	369:400	nonpathogenic fungus N. discreta	369:400	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	0	76	theme	source	20:25	arg1	complexity					27:36	carbon source complexity	13:36	carbon source complexity	13:36	Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.					
31793753	1	77	theme	biofilms	281:288	arg1	microstructure					212:225	microstructure	212:225	microstructure	212:225	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	1	77	theme	biofilms	281:288	arg1	composition					237:247	chemical composition	228:247	chemical composition	228:247	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	1	77	theme	biofilms	281:288	arg1	capacity					269:276	water retention capacity	253:276	water retention capacity	253:276	AIMS To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.					
31793753	7	78	theme	cells	1295:1299	arg1	pathway					1284:1290	the metabolic pathway	1270:1290	the metabolic pathway of cells	1270:1299	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	4	79	theme	cells	735:739	arg1	areas					726:730	relative stained areas	709:730	relative stained areas of cells and carbohydrates from fluorescence imaging of samples	709:794	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
31793753	7	80	dep	CONCLUSIONS	1222:1232	arg1	impacts					1262:1268	impacts	1262:1268	impacts	1262:1268	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	6	81	theme	protein	1130:1136	arg1	content					1138:1144	lower carbohydrate and protein content	1107:1144	content	1138:1144	The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic.					
31793753	3	82	from	increase	460:467	arg1	complexity					472:481	complexity	472:481	complexity of carbon source	472:498	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	3	83	theme	source	493:498	arg1	complexity					472:481	complexity	472:481	complexity of carbon source	472:498	The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms.					
31793753	7	84	theme	source	1255:1260	arg1	nature					1238:1243	The nature	1234:1243	The nature of carbon source	1234:1260	CONCLUSIONS The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells.					
31793753	9	85	dep	STUDY	1480:1484	arg1	shows					1496:1500	shows	1496:1500	shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials	1496:1659	SIGNIFICANCE AND IMPACT OF THE STUDY This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.					
31793753	2	86	theme	nonpathogenic	369:381	arg1	discreta					393:400	nonpathogenic fungus N. discreta	369:400	nonpathogenic fungus N. discreta	369:400	METHODS AND RESULTS Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source.					
31793753	4	87	from	images	697:702	arg1	porosity					670:677	porosity	670:677	porosity from bright-field images	670:702	Confocal laser scanning microscopy was used to calculate porosity from bright-field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488.					
29438752	2	0	theme	1-ethyl-3-	601:610	arg1	solution					682:689	N-hydroxysuccinimide (NHS) solution	655:689	N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS)	655:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	0	theme	1-ethyl-3-	601:610	arg1	EDC					649:651	EDC	649:651	EDC	649:651	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	0	theme	1-ethyl-3-	601:610	arg1	carbodiimide					635:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	601:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	601:652	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	1	theme	important	252:260	arg1	challenge					262:270	an important challenge	249:270	an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential	249:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	6	2	theme	X-ray	1199:1203	arg1	studies					1217:1223	the X-ray diffraction studies	1195:1223	the X-ray diffraction studies	1195:1223	The crystallinity of the scaffold was examined by the X-ray diffraction studies.					
29438752	7	3	theme	cartilage	1383:1391	arg1	regeneration					1400:1411	cartilage tissue regeneration	1383:1411	cartilage tissue regeneration	1383:1411	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	2	4	theme	3-dimethylaminopropyl	612:632	arg1	solution					682:689	N-hydroxysuccinimide (NHS) solution	655:689	N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS)	655:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	4	theme	3-dimethylaminopropyl	612:632	arg1	EDC					649:651	EDC	649:651	EDC	649:651	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	4	theme	3-dimethylaminopropyl	612:632	arg1	carbodiimide					635:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	601:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	601:652	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	7	5	theme	suitable	1350:1357	arg1	strength					1370:1377	suitable mechanical strength	1350:1377	suitable mechanical strength for cartilage tissue regeneration	1350:1411	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	8	6	theme	MTT	1414:1416	arg1	assay					1418:1422	MTT assay	1414:1422	MTT assay	1414:1422	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	6	7	theme	scaffold	1170:1177	arg1	crystallinity					1149:1161	The crystallinity	1145:1161	The crystallinity of the scaffold	1145:1177	The crystallinity of the scaffold was examined by the X-ray diffraction studies.					
29438752	1	8	theme	supplementation	206:220	arg1	lack					198:201	lack	198:201	lack	198:201	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	1	8	theme	supplementation	206:220	arg1	nature					187:192	avascular nature	177:192	avascular nature	177:192	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	8	9	theme	cell	1512:1515	arg1	scaffold					1524:1531	the cell seeded scaffold	1508:1531	the cell seeded scaffold	1508:1531	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	2	10	theme	chondroitin	702:712	arg1	CS					723:724	CS	723:724	CS	723:724	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	10	theme	chondroitin	702:712	arg1	sulfate					714:720	chondroitin sulfate	702:720	chondroitin sulfate (CS)	702:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	11	theme	Poor	118:121	arg1	potential					136:144	Poor regenerative potential	118:144	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells	118:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	2	12	theme	freeze	561:566	arg1	method					575:580	freeze drying method	561:580	freeze drying method	561:580	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	4	13	theme	homogeneous	944:954	arg1	structure					961:969	homogeneous pore structure	944:969	homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%	944:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	7	14	theme	moderate	1282:1289	arg1	biodegradation					1291:1304	moderate biodegradation	1282:1304	moderate biodegradation	1282:1304	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	1	15	theme	matrix	347:352	arg1	development					307:317	development	307:317	development of artificial extracellular matrix	307:352	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	1	16	theme	cartilage	149:157	arg1	tissue					159:164	cartilage tissue	149:164	cartilage tissue due to the avascular nature and lack of supplementation of reparative cells	149:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	0	17	theme	composite	63:71	arg1	scaffolds					73:81	based composite scaffolds	57:81	based composite scaffolds for cartilage tissue regeneration	57:115	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	1	18	theme	enhanced	359:366	arg1	potential					402:410	enhanced neo-cartilage tissue regeneration potential	359:410	enhanced neo-cartilage tissue regeneration potential	359:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	1	19	theme	due	166:168	arg1	tissue					159:164	cartilage tissue	149:164	cartilage tissue due to the avascular nature and lack of supplementation of reparative cells	149:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	2	20	theme	polyelectrolyte	508:522	arg1	complex					524:530	polyelectrolyte complex	508:530	polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM)	508:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	21	theme	tissue	382:387	arg1	potential					402:410	enhanced neo-cartilage tissue regeneration potential	359:410	enhanced neo-cartilage tissue regeneration potential	359:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	8	22	theme	regenerative	1482:1493	arg1	potential					1495:1503	the regenerative potential	1478:1503	the regenerative potential of the cell seeded scaffold	1478:1531	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	0	23	theme	tissue	97:102	arg1	regeneration					104:115	cartilage tissue regeneration	87:115	cartilage tissue regeneration	87:115	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	3	24	theme	mechanical	858:867	arg1	behaviors					869:877	The physical, chemical, thermal, and mechanical behaviors	821:877	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds	821:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	2	25	theme	NHS	677:679	arg1	solution					682:689	N-hydroxysuccinimide (NHS) solution	655:689	N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS)	655:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	25	theme	NHS	677:679	arg1	carbodiimide					635:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	601:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	601:652	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	26	theme	recent	275:280	arg1	practice					290:297	recent medical practice	275:297	recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential	275:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	9	27	theme	histopathological	1538:1554	arg1	analysis					1556:1563	The histopathological analysis	1534:1563	The histopathological analysis	1534:1563	The histopathological analysis defines the suitability of scaffold for cartilage tissue regeneration.					
29438752	7	28	theme	swelling	1263:1270	arg1	property					1272:1279	suitable swelling property	1254:1279	suitable swelling property	1254:1279	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	5	29	dep	polymers	1096:1103	arg1	PC					1119:1120	PC	1119:1120	PC	1119:1120	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	5	29	dep	polymers	1096:1103	arg1	PLLA					1110:1113	PLLA	1110:1113	PLLA	1110:1113	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	5	29	dep	polymers	1096:1103	arg1	CH					1106:1107	CH	1106:1107	CH	1106:1107	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	5	29	dep	polymers	1096:1103	arg1	polymers					1096:1103	polymers	1096:1103	polymers (CH, PLLA and PC)	1096:1121	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	2	30	theme	N-hydroxysuccinimide	655:674	arg1	solution					682:689	N-hydroxysuccinimide (NHS) solution	655:689	N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS)	655:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	30	theme	N-hydroxysuccinimide	655:674	arg1	carbodiimide					635:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	601:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	601:652	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	4	31	from	porosities	999:1008	arg1	range					1017:1021	the range	1013:1021	the range of 79 to 84%	1013:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	3	32	theme	scaffolds	892:900	arg1	behaviors					869:877	The physical, chemical, thermal, and mechanical behaviors	821:877	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds	821:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	4	33	theme	pore	976:979	arg1	size					981:984	pore size 49-170μm	976:993	pore size 49-170μm	976:993	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	8	34	theme	scaffold	1524:1531	arg1	potential					1495:1503	the regenerative potential	1478:1503	the regenerative potential of the cell seeded scaffold	1478:1531	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	2	35	theme	poly	428:431	arg1	compositions					469:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	0	36	theme	chitosan/poly	25:37	arg1	evaluation					11:20	evaluation	11:20	evaluation of chitosan/poly(l-lactide)	11:48	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	0	36	theme	chitosan/poly	25:37	arg1	Design					0:5	Design	0:5	Design	0:5	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	4	37	with	structure	961:969	arg1	size					981:984	pore size 49-170μm	976:993	pore size 49-170μm	976:993	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	4	37	with	structure	961:969	arg1	porosities					999:1008	porosities	999:1008	porosities in the range of 79 to 84%	999:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	1	38	theme	artificial	322:331	arg1	matrix					347:352	artificial extracellular matrix	322:352	artificial extracellular matrix	322:352	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	9	39	theme	scaffold	1592:1599	arg1	suitability					1577:1587	the suitability	1573:1587	the suitability of scaffold for cartilage tissue regeneration	1573:1633	The histopathological analysis defines the suitability of scaffold for cartilage tissue regeneration.					
29438752	4	40	theme	%	1034:1034	arg1	range					1017:1021	the range	1013:1021	the range of 79 to 84%	1013:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	9	41	theme	cartilage	1605:1613	arg1	regeneration					1622:1633	cartilage tissue regeneration	1605:1633	cartilage tissue regeneration	1605:1633	The histopathological analysis defines the suitability of scaffold for cartilage tissue regeneration.					
29438752	4	42	with	porous	932:937	arg1	structure					961:969	homogeneous pore structure	944:969	homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%	944:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	2	43	theme	cartilage	783:791	arg1	ECM					815:817	ECM	815:817	ECM	815:817	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	43	theme	cartilage	783:791	arg1	matrix					807:812	the cartilage extracellular matrix	779:812	the cartilage extracellular matrix (ECM)	779:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	5	44	dep	Fourier	1037:1043	arg1	transform					1045:1053	transform	1045:1053	transform infrared study	1045:1068	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	1	45	theme	cells	236:240	arg1	supplementation					206:220	supplementation	206:220	supplementation of reparative cells	206:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	7	46	from	property	1272:1279	arg1	nature					1331:1336	nature	1331:1336	nature	1331:1336	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	6	47	theme	diffraction	1205:1215	arg1	studies					1217:1223	the X-ray diffraction studies	1195:1223	the X-ray diffraction studies	1195:1223	The crystallinity of the scaffold was examined by the X-ray diffraction studies.					
29438752	7	48	contain	possess	1342:1348	arg1	scaffold					1239:1246	scaffold	1239:1246	scaffold	1239:1246	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	7	48	contain	possess	1342:1348	arg2	strength					1370:1377	suitable mechanical strength	1350:1377	suitable mechanical strength for cartilage tissue regeneration	1350:1411	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	2	49	dep	complex	524:530	arg1	crosslinked					586:596	crosslinked	586:596	crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM)	586:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	49	dep	complex	524:530	arg1	based					532:536	based	532:536	based porous scaffolds using freeze drying method	532:580	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	50	with	practice	290:297	arg1	potential					402:410	enhanced neo-cartilage tissue regeneration potential	359:410	enhanced neo-cartilage tissue regeneration potential	359:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	7	51	theme	tissue	1393:1398	arg1	regeneration					1400:1411	cartilage tissue regeneration	1383:1411	cartilage tissue regeneration	1383:1411	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	1	52	theme	avascular	177:185	arg1	nature					187:192	avascular nature	177:192	avascular nature	177:192	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	1	53	from	challenge	262:270	arg1	practice					290:297	recent medical practice	275:297	recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential	275:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	2	54	theme	porous	538:543	arg1	scaffolds					545:553	porous scaffolds	538:553	porous scaffolds using freeze drying method	538:580	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	7	55	theme	mechanical	1359:1368	arg1	strength					1370:1377	suitable mechanical strength	1350:1377	suitable mechanical strength for cartilage tissue regeneration	1350:1411	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	2	56	theme	matrix	807:812	arg1	composition					740:750	the composition	736:750	the composition as well as architecture of the cartilage extracellular matrix (ECM)	736:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	56	theme	matrix	807:812	arg1	architecture					763:774	architecture	763:774	the composition as well as architecture of the cartilage extracellular matrix (ECM)	736:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	8	57	theme	GAG	1425:1427	arg1	content					1429:1435	GAG content	1425:1435	GAG content	1425:1435	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	5	58	theme	polymers	1096:1103	arg1	presence					1084:1091	the presence	1080:1091	the presence of polymers (CH, PLLA and PC) within the scaffolds	1080:1142	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	2	59	theme	drying	568:573	arg1	method					575:580	freeze drying method	561:580	freeze drying method	561:580	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	60	theme	regenerative	123:134	arg1	potential					136:144	Poor regenerative potential	118:144	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells	118:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	3	61	theme	chemical	835:842	arg1	behaviors					869:877	The physical, chemical, thermal, and mechanical behaviors	821:877	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds	821:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	0	62	theme	based	57:61	arg1	scaffolds					73:81	based composite scaffolds	57:81	based composite scaffolds for cartilage tissue regeneration	57:115	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	3	63	theme	physical	825:832	arg1	behaviors					869:877	The physical, chemical, thermal, and mechanical behaviors	821:877	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds	821:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	8	64	theme	chondrocyte	1456:1466	arg1	assay					1418:1422	MTT assay	1414:1422	MTT assay	1414:1422	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	8	64	theme	chondrocyte	1456:1466	arg1	content					1429:1435	GAG content	1425:1435	GAG content	1425:1435	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	8	64	theme	chondrocyte	1456:1466	arg1	attachment					1442:1451	attachment	1442:1451	attachment of chondrocyte	1442:1466	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	1	65	theme	tissue	159:164	arg1	potential					136:144	Poor regenerative potential	118:144	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells	118:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	4	66	theme	pore	956:959	arg1	structure					961:969	homogeneous pore structure	944:969	homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%	944:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	0	67	theme	cartilage	87:95	arg1	regeneration					104:115	cartilage tissue regeneration	87:115	cartilage tissue regeneration	87:115	Design and evaluation of chitosan/poly(l-lactide)/pectin based composite scaffolds for cartilage tissue regeneration.					
29438752	1	68	theme	neo-cartilage	368:380	arg1	potential					402:410	enhanced neo-cartilage tissue regeneration potential	359:410	enhanced neo-cartilage tissue regeneration potential	359:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	3	69	theme	thermal	845:851	arg1	behaviors					869:877	The physical, chemical, thermal, and mechanical behaviors	821:877	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds	821:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	1	70	theme	regeneration	389:400	arg1	potential					402:410	enhanced neo-cartilage tissue regeneration potential	359:410	enhanced neo-cartilage tissue regeneration potential	359:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	3	71	theme	developed	882:890	arg1	scaffolds					892:900	developed scaffolds	882:900	developed scaffolds	882:900	The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done.					
29438752	7	72	from	hemocompatibility	1310:1326	arg1	nature					1331:1336	nature	1331:1336	nature	1331:1336	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	7	73	theme	suitable	1254:1261	arg1	property					1272:1279	suitable swelling property	1254:1279	suitable swelling property	1254:1279	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	8	74	theme	seeded	1517:1522	arg1	scaffold					1524:1531	the cell seeded scaffold	1508:1531	the cell seeded scaffold	1508:1531	MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold.					
29438752	2	75	theme	Chitosan	413:420	arg1	compositions					469:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	76	theme	medical	282:288	arg1	practice					290:297	recent medical practice	275:297	recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential	275:410	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	4	77	dep	84	1032:1033	arg1	to					1029:1030	to	1029:1030	to	1029:1030	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	7	78	from	biodegradation	1291:1304	arg1	nature					1331:1336	nature	1331:1336	nature	1331:1336	Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration.					
29438752	2	79	theme	pectin	457:462	arg1	compositions					469:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions	413:480	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	80	dep	nature	187:192	arg1	the					173:175	the	173:175	the	173:175	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	4	81	from	size	981:984	arg1	range					1017:1021	the range	1013:1021	the range of 79 to 84%	1013:1034	The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%.					
29438752	5	82	dep	transform	1045:1053	arg1	infrared					1055:1062	infrared	1055:1062	transform infrared study	1045:1068	Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds.					
29438752	1	83	theme	extracellular	333:345	arg1	matrix					347:352	artificial extracellular matrix	322:352	artificial extracellular matrix	322:352	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	2	84	theme	extracellular	793:805	arg1	ECM					815:817	ECM	815:817	ECM	815:817	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	84	theme	extracellular	793:805	arg1	matrix					807:812	the cartilage extracellular matrix	779:812	the cartilage extracellular matrix (ECM)	779:818	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	1	85	theme	reparative	225:234	arg1	cells					236:240	reparative cells	225:240	reparative cells	225:240	Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential.					
29438752	9	86	theme	tissue	1615:1620	arg1	regeneration					1622:1633	cartilage tissue regeneration	1605:1633	cartilage tissue regeneration	1605:1633	The histopathological analysis defines the suitability of scaffold for cartilage tissue regeneration.					
29438752	2	87	contain	containing	691:700	arg1	solution					682:689	N-hydroxysuccinimide (NHS) solution	655:689	N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS)	655:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	87	contain	containing	691:700	arg2	sulfate					714:720	chondroitin sulfate	702:720	chondroitin sulfate (CS)	702:725	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	87	contain	containing	691:700	arg2	CS					723:724	CS	723:724	CS	723:724	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
29438752	2	87	contain	containing	691:700	arg1	carbodiimide					635:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	601:646	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	601:652	Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM).					
30669397	5	0	from	impact	995:1000	arg1	composition					1046:1056	the tomato cell wall composition	1025:1056	the tomato cell wall composition	1025:1056	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	3	1	theme	wall	676:679	arg1	material					681:688	cell wall material	671:688	cell wall material	671:688	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	7	2	theme	modifications	1332:1344	arg1	discernment					1297:1307	the discernment	1293:1307	the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization	1293:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	6	3	theme	inoculation	1216:1226	arg1	effects					1190:1196	the effects	1186:1196	the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage	1186:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	7	4	theme	high	1457:1460	arg1	fertilization					1462:1474	high fertilization	1457:1474	high fertilization	1457:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	7	5	theme	different	1408:1416	arg1	response					1425:1432	a different tomato response	1406:1432	a different tomato response to AM colonization and high fertilization	1406:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	4	6	theme	fruit	880:884	arg1	features					886:893	fruit features	880:893	fruit features	880:893	In addition, to improve host nutrition and tolerance/resistance to environmental stresses, AM symbiosis was shown to modulate fruit features.					
30669397	0	7	from	Changes	14:20	arg1	Walls					37:41	Tomato Cell Walls	25:41	Tomato Cell Walls in Roots and Fruits	25:61	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	3	8	theme	developing	623:632	arg1	structures					655:664	all the developing intracellular fungal structures	615:664	all the developing intracellular fungal structures	615:664	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	5	9	used	used	976:979	arg2	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	10	theme	Microarray	925:934	arg1	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	7	11	theme	cell	1312:1315	arg1	modifications					1332:1344	cell wall component modifications	1312:1344	cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization	1312:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	1	12	theme	plant	244:248	arg1	interactions					250:261	plant interactions	244:261	plant interactions with other organisms, such as the mycorrhizal fungi	244:313	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	5	13	theme	cell	1036:1039	arg1	composition					1046:1056	the tomato cell wall composition	1025:1056	the tomato cell wall composition	1025:1056	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	4	14	theme	AM	845:846	arg1	symbiosis					848:856	AM symbiosis	845:856	AM symbiosis	845:856	In addition, to improve host nutrition and tolerance/resistance to environmental stresses, AM symbiosis was shown to modulate fruit features.					
30669397	5	15	theme	Polymer	936:942	arg1	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	6	16	theme	fruit	1251:1255	arg1	stage					1266:1270	the fruit ripening stage	1247:1270	the fruit ripening stage	1247:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	0	17	theme	Mycorrhizal	95:105	arg1	Colonization					107:118	Arbuscular Mycorrhizal Colonization	84:118	Arbuscular Mycorrhizal Colonization	84:118	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	5	18	theme	wall	1041:1044	arg1	composition					1046:1056	the tomato cell wall composition	1025:1056	the tomato cell wall composition	1025:1056	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	19	theme	Profiling	944:952	arg1	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	2	20	theme	most	364:367	arg1	organisms					385:393	the most widespread soil organisms	360:393	the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon	360:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	0	21	theme	Arbuscular	84:93	arg1	Colonization					107:118	Arbuscular Mycorrhizal Colonization	84:118	Arbuscular Mycorrhizal Colonization	84:118	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	0	22	from	Walls	37:41	arg1	Fruits					56:61	Fruits	56:61	Fruits	56:61	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	0	22	from	Walls	37:41	arg1	Roots					46:50	Roots	46:50	Roots	46:50	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	6	23	theme	ripening	1257:1264	arg1	stage					1266:1270	the fruit ripening stage	1247:1270	the fruit ripening stage	1247:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	1	24	from	Modifications	121:133	arg1	composition					148:158	cell wall composition	138:158	cell wall composition	138:158	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	6	25	theme	stage	1266:1270	arg1	effects					1190:1196	the effects	1186:1196	the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage	1186:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	7	26	theme	mycorrhizal	1372:1382	arg1	colonization					1384:1395	mycorrhizal colonization	1372:1395	mycorrhizal colonization	1372:1395	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	4	27	theme	environmental	821:833	arg1	stresses					835:842	environmental stresses	821:842	environmental stresses	821:842	In addition, to improve host nutrition and tolerance/resistance to environmental stresses, AM symbiosis was shown to modulate fruit features.					
30669397	7	28	theme	wall	1317:1320	arg1	modifications					1332:1344	cell wall component modifications	1312:1344	cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization	1312:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	7	29	theme	AM	1437:1438	arg1	colonization					1440:1451	AM colonization	1437:1451	AM colonization	1437:1451	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	6	30	theme	data	1120:1123	arg1	analyses					1125:1132	Multivariate data analyses	1107:1132	Multivariate data analyses	1107:1132	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	5	31	theme	AM	1009:1010	arg1	symbiosis					1012:1020	the AM symbiosis	1005:1020	the AM symbiosis	1005:1020	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	7	32	theme	tomato	1418:1423	arg1	response					1425:1432	a different tomato response	1406:1432	a different tomato response to AM colonization and high fertilization	1406:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	3	33	theme	AM	540:541	arg1	symbiosis					543:551	AM symbiosis	540:551	AM symbiosis	540:551	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	6	34	theme	fertilization	1201:1213	arg1	effects					1190:1196	the effects	1186:1196	the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage	1186:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	0	35	theme	Tomato	25:30	arg1	Walls					37:41	Tomato Cell Walls	25:41	Tomato Cell Walls in Roots and Fruits	25:61	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	1	36	theme	other	268:272	arg1	organisms					274:282	other organisms	268:282	other organisms	268:282	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	1	36	theme	other	268:272	arg1	fungi					309:313	the mycorrhizal fungi	293:313	the mycorrhizal fungi	293:313	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	2	37	theme	plant-assimilated	511:527	arg1	carbon					529:534	plant-assimilated carbon	511:534	plant-assimilated carbon	511:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	6	38	with	inoculation	1216:1226	arg1	fungi					1236:1240	AM fungi	1233:1240	AM fungi	1233:1240	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	7	39	theme	component	1322:1330	arg1	modifications					1332:1344	cell wall component modifications	1312:1344	cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization	1312:1474	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	5	40	theme	local	1066:1070	arg1	fruit					1099:1103	fruit	1099:1103	fruit	1099:1103	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	40	theme	local	1066:1070	arg1	level					1092:1096	local (root) and systemic level	1066:1096	local (root) and systemic level (fruit)	1066:1104	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	0	41	theme	Colonization	107:118	arg1	Contribution					68:79	The Contribution	64:79	The Contribution of Arbuscular Mycorrhizal Colonization	64:118	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	5	42	theme	symbiosis	1012:1020	arg1	impact					995:1000	the impact	991:1000	the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit)	991:1104	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	43	theme	Comprehensive	911:923	arg1	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	44	dep	local	1066:1070	arg1	root					1073:1076	root	1073:1076	root	1073:1076	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	6	45	theme	obtained	1156:1163	arg1	datasets					1165:1172	the obtained datasets	1152:1172	the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage	1152:1270	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	6	46	theme	Multivariate	1107:1118	arg1	analyses					1125:1132	Multivariate data analyses	1107:1132	Multivariate data analyses	1107:1132	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	2	47	theme	soil	380:383	arg1	organisms					385:393	the most widespread soil organisms	360:393	the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon	360:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	0	48	theme	Cell	32:35	arg1	Walls					37:41	Tomato Cell Walls	25:41	Tomato Cell Walls in Roots and Fruits	25:61	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	3	49	theme	cell	740:743	arg1	surface					745:751	the fungal cell surface	729:751	the fungal cell surface	729:751	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	3	50	theme	host	558:561	arg1	membrane					570:577	the host plasma membrane	554:577	the host plasma membrane	554:577	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	2	51	theme	widespread	369:378	arg1	organisms					385:393	the most widespread soil organisms	360:393	the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon	360:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	2	52	theme	plants	427:432	arg1	roots					413:417	the roots	409:417	the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon	409:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	5	53	dep	composition	1046:1056	arg1	both					1058:1061	both	1058:1061	both	1058:1061	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	54	theme	tomato	1029:1034	arg1	composition					1046:1056	the tomato cell wall composition	1025:1056	the tomato cell wall composition	1025:1056	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	1	55	with	interactions	250:261	arg1	organisms					274:282	other organisms	268:282	other organisms	268:282	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	1	55	with	interactions	250:261	arg1	fungi					309:313	the mycorrhizal fungi	293:313	the mycorrhizal fungi	293:313	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	2	56	theme	mycorrhizal	327:337	arg1	AM					340:341	AM	340:341	AM	340:341	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	2	56	theme	mycorrhizal	327:337	arg1	fungi					344:348	Arbuscular mycorrhizal (AM) fungi	316:348	Arbuscular mycorrhizal (AM) fungi	316:348	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	2	57	theme	land	422:425	arg1	plants					427:432	land plants	422:432	land plants	422:432	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	5	58	theme	systemic	1083:1090	arg1	fruit					1099:1103	fruit	1099:1103	fruit	1099:1103	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	5	58	theme	systemic	1083:1090	arg1	level					1092:1096	local (root) and systemic level	1066:1096	local (root) and systemic level (fruit)	1066:1104	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	6	59	theme	AM	1233:1234	arg1	fungi					1236:1240	AM fungi	1233:1240	AM fungi	1233:1240	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	3	60	theme	fungal	648:653	arg1	structures					655:664	all the developing intracellular fungal structures	615:664	all the developing intracellular fungal structures	615:664	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	4	61	theme	host	778:781	arg1	nutrition					783:791	host nutrition	778:791	host nutrition	778:791	In addition, to improve host nutrition and tolerance/resistance to environmental stresses, AM symbiosis was shown to modulate fruit features.					
30669397	2	62	theme	Arbuscular	316:325	arg1	AM					340:341	AM	340:341	AM	340:341	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	2	62	theme	Arbuscular	316:325	arg1	fungi					344:348	Arbuscular mycorrhizal (AM) fungi	316:348	Arbuscular mycorrhizal (AM) fungi	316:348	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	1	63	theme	mycorrhizal	297:307	arg1	fungi					309:313	the mycorrhizal fungi	293:313	the mycorrhizal fungi	293:313	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	7	64	dep	discernment	1297:1307	arg1	both					1477:1480	both	1477:1480	both	1477:1480	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	5	65	theme	CoMMP	955:959	arg1	technique					962:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	Comprehensive Microarray Polymer Profiling (CoMMP) technique	911:970	In this study, Comprehensive Microarray Polymer Profiling (CoMMP) technique was used to verify the impact of the AM symbiosis on the tomato cell wall composition both at local (root) and systemic level (fruit).					
30669397	1	66	from	changes	189:195	arg1	structure					204:212	its structure	200:212	its structure	200:212	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	7	67	theme	systemic	1502:1509	arg1	level					1511:1515	the root and the systemic level	1485:1515	level	1511:1515	Results allowed for the discernment of cell wall component modifications that were correlated with mycorrhizal colonization, showing a different tomato response to AM colonization and high fertilization, both at the root and the systemic level.					
30669397	3	68	theme	fungal	733:738	arg1	surface					745:751	the fungal cell surface	729:751	the fungal cell surface	729:751	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	2	69	from	soil	490:493	arg1	exchange					498:505	exchange	498:505	exchange for plant-assimilated carbon	498:534	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	6	70	with	stage	1266:1270	arg1	fungi					1236:1240	AM fungi	1233:1240	AM fungi	1233:1240	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	2	71	theme	nutrient	465:472	arg1	uptake					474:479	mineral nutrient uptake	457:479	mineral nutrient uptake	457:479	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	3	72	theme	intracellular	634:646	arg1	structures					655:664	all the developing intracellular fungal structures	615:664	all the developing intracellular fungal structures	615:664	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	3	73	theme	plasma	563:568	arg1	membrane					570:577	the host plasma membrane	554:577	the host plasma membrane	554:577	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	1	74	theme	cell	138:141	arg1	composition					148:158	cell wall composition	138:158	cell wall composition	138:158	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30669397	6	75	with	fertilization	1201:1213	arg1	fungi					1236:1240	AM fungi	1233:1240	AM fungi	1233:1240	Multivariate data analyses were performed on the obtained datasets looking for the effects of fertilization, inoculation with AM fungi, and the fruit ripening stage.					
30669397	2	76	theme	mineral	457:463	arg1	uptake					474:479	mineral nutrient uptake	457:479	mineral nutrient uptake	457:479	Arbuscular mycorrhizal (AM) fungi are among the most widespread soil organisms that colonize the roots of land plants, where they facilitate mineral nutrient uptake from the soil in exchange for plant-assimilated carbon.					
30669397	3	77	theme	cell	671:674	arg1	material					681:688	cell wall material	671:688	cell wall material	671:688	In AM symbiosis, the host plasma membrane invaginates and proliferates around all the developing intracellular fungal structures, and cell wall material is laid down between this membrane and the fungal cell surface.					
30669397	0	78	dep	Understanding	0:12	arg1	Contribution					68:79	The Contribution	64:79	The Contribution of Arbuscular Mycorrhizal Colonization	64:118	Understanding Changes in Tomato Cell Walls in Roots and Fruits: The Contribution of Arbuscular Mycorrhizal Colonization.					
30669397	1	79	theme	wall	143:146	arg1	composition					148:158	cell wall composition	138:158	cell wall composition	138:158	Modifications in cell wall composition, which can be accompanied by changes in its structure, were already reported during plant interactions with other organisms, such as the mycorrhizal fungi.					
30248961	0	0	theme	Main	69:72	arg1	Polysaccharide					74:87	a Main Polysaccharide	67:87	a Main Polysaccharide in Pollen of Chinese Wolfberry	67:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	0	1	from	Characterization	11:26	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	4	2	from	arabinose	754:762	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	5	3	theme	MTT	870:872	arg1	assay					881:885	MTT, Tunel assay	870:885	assay	881:885	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	7	4	theme	Tunel	1093:1097	arg1	assay					1099:1103	Tunel assay	1093:1103	Tunel assay	1093:1103	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	6	5	theme	DU145	1064:1068	arg1	cells					1086:1090	DU145 prostate cancer cells	1064:1090	DU145 prostate cancer cells	1064:1090	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	2	6	theme	hot-water	318:326	arg1	extraction					328:337	hot-water extraction	318:337	hot-water extraction	318:337	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	8	7	theme	agent	1316:1320	arg1	source					1277:1282	a potential source	1265:1282	a potential source of antitumor functional food or agent	1265:1320	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	8	7	theme	agent	1316:1320	arg1	CF1					1244:1246	the CF1	1240:1246	the CF1 of WPPs	1240:1254	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	0	8	from	Isolation	0:8	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	4	9	theme	molar	785:789	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	2	10	dep	extracted	305:313	arg1	purified					374:381	purified	374:381	further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column	366:452	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	0	11	theme	Polysaccharide	74:87	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	8	12	theme	present	1211:1217	arg1	study					1219:1223	The present study	1207:1223	The present study	1207:1223	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	3	13	theme	which	532:536	arg1	kDa					574:576	1540.10 ± 48.78 kDa	558:576	1540.10 ± 48.78 kDa by HPGPC-ELSD	558:590	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	3	13	theme	which	532:536	arg1	weight					522:527	the molecular weight	508:527	the molecular weight	508:527	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	4	14	theme	glucuronic	699:708	arg1	acid					710:713	glucuronic acid	699:713	glucuronic acid	699:713	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	4	15	theme	CF1	681:683	arg1	compositions					665:676	the monosaccharide compositions	646:676	the monosaccharide compositions of CF1	646:683	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	4	15	theme	CF1	681:683	arg1	mannose					690:696	mannose	690:696	mannose	690:696	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	7	16	dep	assay	1099:1103	arg1	assay					1124:1128	assay	1124:1128	assay	1124:1128	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	4	17	from	xylose	735:740	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	2	18	theme	pollen	247:252	arg1	polysaccharides					254:268	pollen polysaccharides	247:268	pollen polysaccharides from Chinese wolfberry (WPPs)	247:298	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	6	19	theme	374.11	1043:1048	arg1	μg/mL					1050:1054	374.11 μg/mL	1043:1054	374.11 μg/mL	1043:1054	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	0	20	from	Effect	42:47	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	8	21	theme	antitumor	1287:1295	arg1	food					1308:1311	antitumor functional food	1287:1311	antitumor functional food	1287:1311	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	5	22	theme	cytometry	896:904	arg1	assay					906:910	flow cytometry assay	891:910	flow cytometry assay	891:910	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	6	23	theme	μg/mL	1050:1054	arg1	value					1034:1038	an IC50 value	1026:1038	an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells	1026:1090	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	5	24	theme	antitumor	840:848	arg1	effects					850:856	The antitumor effects	836:856	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay	836:910	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	2	25	theme	Sephadex	432:439	arg1	column					447:452	Sephadex G-100 column	432:452	Sephadex G-100 column	432:452	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	4	26	from	acid	729:732	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	4	27	theme	galacturonic	716:727	arg1	acid					729:732	galacturonic acid	716:732	galacturonic acid	716:732	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	3	28	theme	molecular	512:520	arg1	kDa					574:576	1540.10 ± 48.78 kDa	558:576	1540.10 ± 48.78 kDa by HPGPC-ELSD	558:590	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	3	28	theme	molecular	512:520	arg1	weight					522:527	the molecular weight	508:527	the molecular weight	508:527	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	4	29	theme	monosaccharide	650:663	arg1	compositions					665:676	the monosaccharide compositions	646:676	the monosaccharide compositions of CF1	646:683	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	4	29	theme	monosaccharide	650:663	arg1	mannose					690:696	mannose	690:696	mannose	690:696	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	6	30	theme	dose-dependent	980:993	arg1	effect					1013:1018	a dose-dependent antiproliferative effect	978:1018	a dose-dependent antiproliferative effect	978:1018	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	4	31	theme	0.68:0.59:0.27:0.24:0.22:0.67:0.08	800:833	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	8	32	theme	food	1308:1311	arg1	source					1277:1282	a potential source	1265:1282	a potential source of antitumor functional food or agent	1265:1320	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	8	32	theme	food	1308:1311	arg1	CF1					1244:1246	the CF1	1240:1246	the CF1 of WPPs	1240:1254	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	8	33	theme	functional	1297:1306	arg1	food					1308:1311	antitumor functional food	1287:1311	antitumor functional food	1287:1311	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	1	34	theme	certain	165:171	arg1	role					173:176	a certain role	163:176	a certain role	163:176	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	8	35	theme	WPPs	1251:1254	arg1	source					1277:1282	a potential source	1265:1282	a potential source of antitumor functional food or agent	1265:1320	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	8	35	theme	WPPs	1251:1254	arg1	CF1					1244:1246	the CF1	1240:1246	the CF1 of WPPs	1240:1254	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	0	36	theme	Wolfberry	110:118	arg1	Pollen					92:97	Pollen	92:97	Pollen of Chinese Wolfberry	92:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	5	37	theme	flow	891:894	arg1	assay					906:910	flow cytometry assay	891:910	flow cytometry assay	891:910	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	4	38	theme	PMP	603:605	arg1	analysis					622:629	PMP derivatization analysis	603:629	PMP derivatization analysis	603:629	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	2	39	theme	DEAE-cellulose	406:419	arg1	column					421:426	a DEAE-cellulose column	404:426	a DEAE-cellulose column	404:426	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	0	40	theme	Chinese	102:108	arg1	Wolfberry					110:118	Chinese Wolfberry	102:118	Chinese Wolfberry	102:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	2	41	theme	Chinese	275:281	arg1	wolfberry					283:291	Chinese wolfberry	275:291	Chinese wolfberry (WPPs)	275:298	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	2	41	theme	Chinese	275:281	arg1	WPPs					294:297	WPPs	294:297	WPPs	294:297	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	3	42	theme	Homogeneous	455:465	arg1	CF1					482:484	Homogeneous polysaccharide CF1	455:484	Homogeneous polysaccharide CF1 of WPPS	455:492	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	7	43	theme	flow	1109:1112	arg1	cytometry					1114:1122	flow cytometry	1109:1122	flow cytometry	1109:1122	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	4	44	with	HPLC	593:596	arg1	analysis					622:629	PMP derivatization analysis	603:629	PMP derivatization analysis	603:629	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	6	45	theme	IC50	1029:1032	arg1	value					1034:1038	an IC50 value	1026:1038	an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells	1026:1090	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	0	46	theme	Antitumor	32:40	arg1	Effect					42:47	Antitumor Effect	32:47	Antitumor Effect	32:47	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	1	47	contain	has	159:161	arg1	pollen					152:157	pollen	152:157	pollen	152:157	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	1	47	contain	has	159:161	arg2	role					173:176	a certain role	163:176	a certain role	163:176	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	4	48	theme	derivatization	607:620	arg1	analysis					622:629	PMP derivatization analysis	603:629	PMP derivatization analysis	603:629	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	0	49	theme	DU145	52:56	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	5	50	theme	CF1	861:863	arg1	effects					850:856	The antitumor effects	836:856	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay	836:910	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	0	51	from	Cells	58:62	arg1	Pollen					92:97	Pollen	92:97	Pollen of Chinese Wolfberry	92:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	3	52	theme	1540.10	558:564	arg1	kDa					574:576	1540.10 ± 48.78 kDa	558:576	1540.10 ± 48.78 kDa by HPGPC-ELSD	558:590	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	3	52	theme	1540.10	558:564	arg1	weight					522:527	the molecular weight	508:527	the molecular weight	508:527	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	6	53	theme	prostate	1070:1077	arg1	cells					1086:1090	DU145 prostate cancer cells	1064:1090	DU145 prostate cancer cells	1064:1090	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	2	54	theme	G-100	441:445	arg1	column					447:452	Sephadex G-100 column	432:452	Sephadex G-100 column	432:452	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	3	55	theme	±	566:566	arg1	kDa					574:576	1540.10 ± 48.78 kDa	558:576	1540.10 ± 48.78 kDa by HPGPC-ELSD	558:590	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	3	55	theme	±	566:566	arg1	weight					522:527	the molecular weight	508:527	the molecular weight	508:527	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	3	56	theme	WPPS	489:492	arg1	CF1					482:484	Homogeneous polysaccharide CF1	455:484	Homogeneous polysaccharide CF1 of WPPS	455:492	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	4	57	from	acid	710:713	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	0	58	from	Pollen	92:97	arg1	Cells					58:62	DU145 Cells	52:62	DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry	52:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	4	59	from	galactose	743:751	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	3	60	theme	polysaccharide	467:480	arg1	CF1					482:484	Homogeneous polysaccharide CF1	455:484	Homogeneous polysaccharide CF1 of WPPS	455:492	Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD.					
30248961	6	61	theme	cancer	1079:1084	arg1	cells					1086:1090	DU145 prostate cancer cells	1064:1090	DU145 prostate cancer cells	1064:1090	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	6	62	theme	antiproliferative	995:1011	arg1	effect					1013:1018	a dose-dependent antiproliferative effect	978:1018	a dose-dependent antiproliferative effect	978:1018	The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells.					
30248961	5	63	dep	assay	881:885	arg1	Tunel					875:879	MTT, Tunel assay	870:885	Tunel	875:879	The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro.					
30248961	2	64	theme	present	232:238	arg1	study					240:244	the present study	228:244	the present study	228:244	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	2	65	from	chromatography	386:399	arg1	column					447:452	Sephadex G-100 column	432:452	Sephadex G-100 column	432:452	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	2	65	from	chromatography	386:399	arg1	column					421:426	a DEAE-cellulose column	404:426	a DEAE-cellulose column	404:426	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	1	66	theme	Modern	121:126	arg1	studies					128:134	Modern studies	121:134	Modern studies	121:134	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	8	67	theme	potential	1267:1275	arg1	source					1277:1282	a potential source	1265:1282	a potential source of antitumor functional food or agent	1265:1320	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	8	67	theme	potential	1267:1275	arg1	CF1					1244:1246	the CF1	1240:1246	the CF1 of WPPs	1240:1254	The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.					
30248961	1	68	theme	prostate-related	198:213	arg1	diseases					215:222	prostate-related diseases	198:222	prostate-related diseases	198:222	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	0	69	from	Polysaccharide	74:87	arg1	Pollen					92:97	Pollen	92:97	Pollen of Chinese Wolfberry	92:118	Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.					
30248961	2	70	theme	ethanol	343:349	arg1	precipitation					351:363	ethanol precipitation	343:363	ethanol precipitation	343:363	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	4	71	from	mannose	690:696	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	1	72	theme	diseases	215:222	arg1	treatment					185:193	the treatment	181:193	the treatment of prostate-related diseases	181:222	Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases.					
30248961	7	73	theme	antitumor	1146:1154	arg1	related					1176:1182	related	1176:1182	related	1176:1182	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	7	73	theme	antitumor	1146:1154	arg1	activity					1156:1163	the antitumor activity	1142:1163	the antitumor activity of CF1	1142:1170	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	4	74	from	trehalose	769:777	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08	783:833	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08.					
30248961	2	75	from	wolfberry	283:291	arg1	polysaccharides					254:268	pollen polysaccharides	247:268	pollen polysaccharides from Chinese wolfberry (WPPs)	247:298	In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column.					
30248961	7	76	theme	CF1	1168:1170	arg1	related					1176:1182	related	1176:1182	related	1176:1182	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30248961	7	76	theme	CF1	1168:1170	arg1	activity					1156:1163	the antitumor activity	1142:1163	the antitumor activity of CF1	1142:1170	Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro.					
30461419	5	0	theme	pool	1148:1151	arg1	complexity					1153:1162	compositional, informational and structural pool complexity	1104:1162	compositional, informational and structural pool complexity	1104:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	1	1	theme	polymer	168:174	arg1	strands					176:182	different genetic polymer strands	150:182	different genetic polymer strands	150:182	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	5	2	theme	concomitant	1080:1090	arg1	increase					1092:1099	a concomitant increase	1078:1099	a concomitant increase in compositional, informational and structural pool complexity	1078:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	5	3	theme	capacity	1024:1031	arg1	determinant					999:1009	the fundamental determinant	983:1009	the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential	983:1195	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	4	theme	vicinal	836:842	arg1	configuration					858:870	the vicinal ring cis-diol configuration	832:870	the vicinal ring cis-diol configuration shared by RNA and AtNA	832:893	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	3	5	theme	RNA	526:528	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	4	6	theme	lesser	693:698	arg1	extent					700:705	a lesser extent	691:705	a lesser extent	691:705	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	5	7	theme	evolutionary	1174:1185	arg1	potential					1187:1195	evolutionary potential	1174:1195	evolutionary potential	1174:1195	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	3	8	theme	unnatural	543:551	arg1	polymers					561:568	the unnatural genetic polymers	539:568	the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids)	539:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	8	theme	unnatural	543:551	arg1	AtNA					605:608	AtNA	605:608	AtNA (altritol-nucleic acids)	605:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	8	theme	unnatural	543:551	arg1	HNA					586:588	HNA	586:588	HNA (hexitol-)	586:599	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	8	theme	unnatural	543:551	arg1	ANA					570:572	ANA	570:572	ANA (arabino-)	570:583	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	2	9	theme	non-enzymatic	367:379	arg1	recombination					381:393	non-enzymatic recombination	367:393	non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools	367:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	2	9	theme	non-enzymatic	367:379	arg1	ligation					400:407	ligation	400:407	ligation	400:407	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	3	10	theme	semi-random	489:499	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	11	theme	DNA	531:533	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	4	12	theme	recombination	773:785	arg1	modes					737:741	diverse modes	729:741	diverse modes of spontaneous intermolecular recombination	729:785	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	5	13	dep	increase	1092:1099	arg1	potential					1187:1195	evolutionary potential	1174:1195	evolutionary potential	1174:1195	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	0	14	theme	genetic	16:22	arg1	pools					33:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.					
30461419	0	15	theme	Random-sequence	0:14	arg1	pools					33:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.					
30461419	5	16	theme	innate	1017:1022	arg1	capacity					1024:1031	an innate capacity	1014:1031	an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential	1014:1195	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	17	theme	RNA	679:681	arg1	pools					713:717	RNA (and to a lesser extent AtNA) pools	679:717	RNA (and to a lesser extent AtNA) pools	679:717	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	1	18	theme	fundamental	191:201	arg1	importance					203:212	fundamental importance	191:212	fundamental importance in biology	191:223	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	1	19	theme	dedicated	295:303	arg1	enzymes					317:323	dedicated recombinase enzymes	295:323	dedicated recombinase enzymes	295:323	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	2	20	from	recombination	381:393	arg1	pools					446:450	random-sequence genetic oligomer pools	413:450	random-sequence genetic oligomer pools	413:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	1	21	dep	different	150:158	arg1	genetic					160:166	genetic	160:166	genetic	160:166	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	0	22	theme	oligomer	24:31	arg1	pools					33:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools	0:37	Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.					
30461419	1	23	theme	recombinase	305:315	arg1	enzymes					317:323	dedicated recombinase enzymes	295:323	dedicated recombinase enzymes	295:323	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	3	24	theme	altritol-nucleic	611:626	arg1	AtNA					605:608	AtNA	605:608	AtNA (altritol-nucleic acids)	605:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	24	theme	altritol-nucleic	611:626	arg1	acids					628:632	altritol-nucleic acids	611:632	altritol-nucleic acids	611:632	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	25	theme	polymers	561:568	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	2	26	theme	oligomer	437:444	arg1	pools					446:450	random-sequence genetic oligomer pools	413:450	random-sequence genetic oligomer pools	413:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	2	27	theme	genetic	429:435	arg1	pools					446:450	random-sequence genetic oligomer pools	413:450	random-sequence genetic oligomer pools	413:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	5	28	from	increase	1092:1099	arg1	complexity					1153:1162	compositional, informational and structural pool complexity	1104:1162	compositional, informational and structural pool complexity	1104:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	2	29	theme	random-sequence	413:427	arg1	pools					446:450	random-sequence genetic oligomer pools	413:450	random-sequence genetic oligomer pools	413:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	5	30	theme	compositional	1104:1116	arg1	complexity					1153:1162	compositional, informational and structural pool complexity	1104:1162	compositional, informational and structural pool complexity	1104:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	31	theme	cis-diol	849:856	arg1	configuration					858:870	the vicinal ring cis-diol configuration	832:870	the vicinal ring cis-diol configuration shared by RNA and AtNA	832:893	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	5	32	theme	structural	1137:1146	arg1	complexity					1153:1162	compositional, informational and structural pool complexity	1104:1162	compositional, informational and structural pool complexity	1104:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	33	theme	intermolecular	758:771	arg1	recombination					773:785	spontaneous intermolecular recombination	746:785	spontaneous intermolecular recombination	746:785	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	3	34	theme	N20	512:514	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	0	35	theme	innate	50:55	arg1	potential					57:65	an innate potential	47:65	an innate potential for ligation and recombination	47:96	Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.					
30461419	4	36	theme	ring	844:847	arg1	configuration					858:870	the vicinal ring cis-diol configuration	832:870	the vicinal ring cis-diol configuration shared by RNA and AtNA	832:893	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	5	37	theme	fundamental	987:997	arg1	determinant					999:1009	the fundamental determinant	983:1009	the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential	983:1195	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	38	theme	spontaneous	746:756	arg1	recombination					773:785	spontaneous intermolecular recombination	746:785	spontaneous intermolecular recombination	746:785	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	1	39	from	importance	203:212	arg1	biology					217:223	biology	217:223	biology	217:223	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	2	40	theme	innate	347:352	arg1	capacity					354:361	an innate capacity	344:361	an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools	344:450	Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools.					
30461419	5	41	theme	informational	1119:1131	arg1	complexity					1153:1162	compositional, informational and structural pool complexity	1104:1162	compositional, informational and structural pool complexity	1104:1162	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	4	42	dep	DNA	642:644	arg1	pools					659:663	pools	659:663	pools	659:663	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	1	43	theme	information	130:140	arg1	Recombination					99:111	Recombination	99:111	Recombination	99:111	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	1	43	theme	information	130:140	arg1	exchange					118:125	the exchange	114:125	the exchange of information between different genetic polymer strands	114:182	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	3	44	theme	genetic	553:559	arg1	polymers					561:568	the unnatural genetic polymers	539:568	the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids)	539:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	44	theme	genetic	553:559	arg1	AtNA					605:608	AtNA	605:608	AtNA (altritol-nucleic acids)	605:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	44	theme	genetic	553:559	arg1	HNA					586:588	HNA	586:588	HNA (hexitol-)	586:599	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	44	theme	genetic	553:559	arg1	ANA					570:572	ANA	570:572	ANA (arabino-)	570:583	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	1	45	theme	genome	229:234	arg1	maintenance					236:246	genome maintenance	229:246	genome maintenance	229:246	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	5	46	theme	chemical	906:913	arg1	constitution					915:926	the chemical constitution	902:926	the chemical constitution that renders both susceptible to hydrolysis	902:970	Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.					
30461419	3	47	theme	eicosamer	501:509	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	48	theme	random	478:483	arg1	pools					517:521	random and semi-random eicosamer (N20) pools	478:521	pools	517:521	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	4	49	theme	diverse	729:735	arg1	modes					737:741	diverse modes	729:741	diverse modes of spontaneous intermolecular recombination	729:785	While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA.					
30461419	1	50	theme	different	150:158	arg1	strands					176:182	different genetic polymer strands	150:182	different genetic polymer strands	150:182	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
30461419	3	51	dep	polymers	561:568	arg1	AtNA					605:608	AtNA	605:608	AtNA (altritol-nucleic acids)	605:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	HNA					586:588	HNA	586:588	HNA (hexitol-)	586:599	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	polymers					561:568	the unnatural genetic polymers	539:568	the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids)	539:633	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	acids					628:632	altritol-nucleic acids	611:632	altritol-nucleic acids	611:632	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	ANA					570:572	ANA	570:572	ANA (arabino-)	570:583	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	hexitol-					591:598	hexitol-	591:598	hexitol-	591:598	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	3	51	dep	polymers	561:568	arg1	arabino-					575:582	arabino-	575:582	arabino-	575:582	Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids).					
30461419	1	52	theme	genetic	252:258	arg1	diversification					260:274	genetic diversification	252:274	genetic diversification	252:274	Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes.					
29654860	8	0	theme	hypoglycemic	914:925	arg1	CCP					895:897	CCP	895:897	CCP	895:897	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	8	0	theme	hypoglycemic	914:925	arg1	medicine					927:934	hypoglycemic medicine	914:934	hypoglycemic medicine	914:934	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	6	1	theme	metformin	676:684	arg1	hydrochloride					686:698	the metformin hydrochloride	672:698	the metformin hydrochloride	672:698	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	5	2	theme	conjugated	402:411	arg1	proteins					413:420	conjugated proteins	402:420	conjugated proteins	402:420	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	0	3	from	activity	13:20	arg1	Abel					73:76	Abel	73:76	Abel	73:76	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel.					
29654860	8	4	theme	health	939:944	arg1	food					946:949	health food	939:949	health food	939:949	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	8	4	theme	health	939:944	arg1	CCP					895:897	CCP	895:897	CCP	895:897	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	7	5	theme	hypoglycemia	823:834	arg1	polysaccharides					836:850	hypoglycemia polysaccharides	823:850	hypoglycemia polysaccharides	823:850	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	6	6	dep	highest	633:639	arg1	exceeded					655:662	exceeded	655:662	exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration	655:766	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	7	7	theme	polysaccharides	836:850	arg1	relationship					801:812	A meaningful structure activity relationship	769:812	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides	769:850	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	5	8	theme	glucose	502:508	arg1	consumption					483:493	the consumption	479:493	the consumption of the glucose in the medium by HepG2 cells	479:537	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	3	9	theme	hypoglycemic	195:206	arg1	activity					208:215	its hypoglycemic activity	191:215	its hypoglycemic activity	191:215	seed cake (CCP) and studied its hypoglycemic activity in vitro.					
29654860	6	10	theme	Tea	720:722	arg1	Polysaccharide					724:737	Tea Polysaccharide	720:737	Tea Polysaccharide	720:737	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	7	11	dep	relationship	801:812	arg1	SAR					815:817	SAR	815:817	SAR	815:817	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	3	12	theme	seed	163:166	arg1	cake					168:171	seed cake	163:171	seed cake (CCP)	163:177	seed cake (CCP) and studied its hypoglycemic activity in vitro.					
29654860	3	12	theme	seed	163:166	arg1	CCP					174:176	CCP	174:176	CCP	174:176	seed cake (CCP) and studied its hypoglycemic activity in vitro.					
29654860	5	13	theme	acidic	438:443	arg1	polysaccharide					445:458	acidic polysaccharide	438:458	acidic polysaccharide	438:458	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	6	14	theme	consumption	587:597	arg1	highest					633:639	highest	633:639	highest	633:639	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	14	theme	consumption	587:597	arg1	RCR					605:607	RCR	605:607	RCR	605:607	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	14	theme	consumption	587:597	arg1	rate					599:602	Relative consumption rate	578:602	Relative consumption rate (RCR) of the glucose	578:623	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	4	15	theme	monosaccharide	275:288	arg1	ratio					308:312	the monosaccharide molar composition ratio	271:312	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	5	16	from	medium	517:522	arg1	consumption					483:493	the consumption	479:493	the consumption of the glucose in the medium by HepG2 cells	479:537	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	5	17	from	glucose	502:508	arg1	medium					517:522	the medium	513:522	the medium	513:522	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	0	18	theme	Hypoglycemic	0:11	arg1	activity					13:20	Hypoglycemic activity	0:20	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel	0:76	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel.					
29654860	7	19	theme	activity	792:799	arg1	relationship					801:812	A meaningful structure activity relationship	769:812	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides	769:850	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	6	20	theme	Relative	578:585	arg1	highest					633:639	highest	633:639	highest	633:639	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	20	theme	Relative	578:585	arg1	RCR					605:607	RCR	605:607	RCR	605:607	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	20	theme	Relative	578:585	arg1	rate					599:602	Relative consumption rate	578:602	Relative consumption rate (RCR) of the glucose	578:623	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	5	21	theme	0.125-0.500 mg/mL	559:575	arg1	range					550:554	the range	546:554	the range of 0.125-0.500 mg/mL	546:575	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	7	22	theme	structure	782:790	arg1	relationship					801:812	A meaningful structure activity relationship	769:812	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides	769:850	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	7	23	theme	meaningful	771:780	arg1	relationship					801:812	A meaningful structure activity relationship	769:812	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides	769:850	A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established.					
29654860	0	24	theme	polysaccharides	34:48	arg1	activity					13:20	Hypoglycemic activity	0:20	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel	0:76	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel.					
29654860	4	25	dep	ratio	308:312	arg1	Galactosamine					342:354	Galactosamine	342:354	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	25	dep	ratio	308:312	arg1	Xylose					319:324	Xylose	319:324	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	25	dep	ratio	308:312	arg1	acid					337:340	Glucuronic acid	326:340	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	25	dep	ratio	308:312	arg1	Mannose = 10.9:4.4:2.6:1.8					356:381	Mannose = 10.9:4.4:2.6:1.8	356:381	Mannose = 10.9:4.4:2.6:1.8	356:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	8	26	used	used	906:909	arg2	medicine					927:934	hypoglycemic medicine	914:934	hypoglycemic medicine	914:934	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	8	26	used	used	906:909	arg2	CCP					895:897	CCP	895:897	CCP	895:897	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	8	26	used	used	906:909	arg2	food					946:949	health food	939:949	health food	939:949	This result suggests that CCP can be used as hypoglycemic medicine or health food.					
29654860	4	27	theme	CCP	251:253	arg1	weight					241:246	The molecular weight	227:246	The molecular weight of CCP	227:253	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	27	theme	CCP	251:253	arg1	4736 Da					259:265	4736 Da	259:265	4736 Da	259:265	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	0	28	from	Abel	73:76	arg1	polysaccharides					34:48	polysaccharides	34:48	polysaccharides from Camellia oleifera Abel	34:76	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel.					
29654860	0	28	from	Abel	73:76	arg1	activity					13:20	Hypoglycemic activity	0:20	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel	0:76	Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel.					
29654860	6	29	theme	same	749:752	arg1	concentration					754:766	the same concentration	745:766	the same concentration	745:766	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	5	30	theme	HepG2	527:531	arg1	cells					533:537	HepG2 cells	527:537	HepG2 cells	527:537	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	4	31	theme	Glucuronic	326:335	arg1	acid					337:340	Glucuronic acid	326:340	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	6	32	theme	glucose	617:623	arg1	highest					633:639	highest	633:639	highest	633:639	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	32	theme	glucose	617:623	arg1	RCR					605:607	RCR	605:607	RCR	605:607	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	6	32	theme	glucose	617:623	arg1	rate					599:602	Relative consumption rate	578:602	Relative consumption rate (RCR) of the glucose	578:623	Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration.					
29654860	5	33	contain	contained	392:400	arg2	proteins					413:420	conjugated proteins	402:420	conjugated proteins	402:420	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	5	33	contain	contained	392:400	arg1	CCP					388:390	The CCP	384:390	The CCP	384:390	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29654860	4	34	theme	composition	296:306	arg1	ratio					308:312	the monosaccharide molar composition ratio	271:312	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	35	theme	molecular	231:239	arg1	weight					241:246	The molecular weight	227:246	The molecular weight of CCP	227:253	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	35	theme	molecular	231:239	arg1	4736 Da					259:265	4736 Da	259:265	4736 Da	259:265	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	4	36	theme	molar	290:294	arg1	ratio					308:312	the monosaccharide molar composition ratio	271:312	the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8	271:381	The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8.					
29654860	5	37	from	consumption	483:493	arg1	medium					517:522	the medium	513:522	the medium	513:522	The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL.					
29983173	2	0	theme	polysaccharide	433:446	arg1	contents					448:455	GAG and general polysaccharide contents	417:455	GAG and general polysaccharide contents in clam tissue	417:470	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	6	1	theme	sugar	1212:1216	arg1	determinations					1188:1201	the determinations	1184:1201	the determinations of amino sugar and uronic acid	1184:1232	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	0	2	from	scarcity	29:36	arg1	tissue					72:77	the soft tissue	63:77	the soft tissue of clam	63:85	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	7	3	theme	sparse	1304:1309	arg1	amounts					1311:1317	sparse amounts	1304:1317	sparse amounts of GAGs	1304:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	3	theme	sparse	1304:1309	arg1	GAGs					1322:1325	GAGs	1322:1325	GAGs	1322:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	2	4	theme	general	425:431	arg1	polysaccharide					433:446	general polysaccharide	425:446	general polysaccharide	425:446	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	3	5	theme	GAGs	667:670	arg1	generation					653:662	trifling generation	644:662	trifling generation of GAGs	644:670	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	3	5	theme	GAGs	667:670	arg1	existence					615:623	the general existence	603:623	the general existence of saccharides	603:638	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	1	6	from	presence	134:141	arg1	tissue					215:220	Meretrix meretrix soft tissue	192:220	Meretrix meretrix soft tissue	192:220	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	4	7	theme	mantle	761:766	arg1	tissues					777:783	the mantle and foot tissues	757:783	the mantle and foot tissues in mucus form	757:797	GAGs coexisting with glycogens appeared to be primarily produced in the mantle and foot tissues in mucus form by visualization.					
29983173	1	8	theme	GAG	237:239	arg1	composition					241:251	GAG composition	237:251	GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera	237:337	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	0	9	from	Abundance	0:8	arg1	tissue					72:77	the soft tissue	63:77	the soft tissue of clam	63:85	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	6	10	theme	acid	1229:1232	arg1	determinations					1188:1201	the determinations	1184:1201	the determinations of amino sugar and uronic acid	1184:1232	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	8	11	theme	subsequent	1357:1366	arg1	development					1368:1378	the subsequent development	1353:1378	the subsequent development of products made from the polysaccharide components of M. meretrix	1353:1445	The results will benefit the subsequent development of products made from the polysaccharide components of M. meretrix.					
29983173	5	12	theme	materials	917:925	arg1	10 g					894:897	10 g	894:897	10 g of 5 dried tissue materials	894:925	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	5	13	theme	tissue	910:915	arg1	materials					917:925	5 dried tissue materials	902:925	5 dried tissue materials	902:925	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	0	14	theme	clam	82:85	arg1	tissue					72:77	the soft tissue	63:77	the soft tissue of clam	63:85	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	5	15	theme	major	1042:1046	arg1	components					1048:1057	the major components	1038:1057	the major components of polysaccharide extracts	1038:1084	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	5	15	theme	major	1042:1046	arg1	GAGs					1024:1027	GAGs	1024:1027	GAGs	1024:1027	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	6	16	theme	uronic	1222:1227	arg1	acid					1229:1232	uronic acid	1222:1232	uronic acid	1222:1232	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	5	17	from	level	965:969	arg1	range					978:982	the range	974:982	the range of 500-1760 mg	974:997	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	1	18	theme	adductor	310:317	arg1	parts					270:274	the different parts	256:274	the different parts	256:274	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	18	theme	adductor	310:317	arg1	muscle					319:324	adductor muscle	310:324	adductor muscle	310:324	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	7	19	theme	clam	1255:1258	arg1	tissues					1244:1250	The soft tissues	1235:1250	The soft tissues of clam	1235:1258	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	20	theme	saccharide	1279:1288	arg1	compounds					1290:1298	abundant saccharide compounds	1270:1298	abundant saccharide compounds	1270:1298	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	2	21	theme	clam	460:463	arg1	tissue					465:470	clam tissue	460:470	clam tissue	460:470	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	5	22	theme	extracts	1077:1084	arg1	components					1048:1057	the major components	1038:1057	the major components of polysaccharide extracts	1038:1084	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	5	22	theme	extracts	1077:1084	arg1	GAGs					1024:1027	GAGs	1024:1027	GAGs	1024:1027	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	5	23	theme	carbohydrate	952:963	arg1	level					965:969	total carbohydrate level	946:969	total carbohydrate level in the range of 500-1760 mg	946:997	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	1	24	attach	presence	134:141	arg1	tissue					215:220	Meretrix meretrix soft tissue	192:220	Meretrix meretrix soft tissue	192:220	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	24	attach	presence	134:141	arg2	GAGs					183:186	GAGs	183:186	GAGs	183:186	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	24	attach	presence	134:141	arg2	glycosaminoglycans					163:180	glycosaminoglycans	163:180	glycosaminoglycans (GAGs)	163:187	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	6	25	theme	amino	1206:1210	arg1	sugar					1212:1216	amino sugar	1206:1216	amino sugar	1206:1216	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	8	26	theme	products	1383:1390	arg1	development					1368:1378	the subsequent development	1353:1378	the subsequent development of products made from the polysaccharide components of M. meretrix	1353:1445	The results will benefit the subsequent development of products made from the polysaccharide components of M. meretrix.					
29983173	0	27	from	glycosaminoglycans	41:58	arg1	tissue					72:77	the soft tissue	63:77	the soft tissue of clam	63:85	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	1	28	theme	glycosaminoglycans	163:180	arg1	distribution					147:158	distribution	147:158	distribution	147:158	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	28	theme	glycosaminoglycans	163:180	arg1	presence					134:141	presence	134:141	presence	134:141	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	6	29	theme	components	1155:1164	arg1	%					1137:1137	approximately 4%	1122:1137	approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid	1122:1232	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	6	29	theme	components	1155:1164	arg1	components					1155:1164	total glycan components	1142:1164	total glycan components	1142:1164	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	8	30	theme	meretrix	1438:1445	arg1	components					1421:1430	the polysaccharide components	1402:1430	the polysaccharide components of M. meretrix	1402:1445	The results will benefit the subsequent development of products made from the polysaccharide components of M. meretrix.					
29983173	3	31	theme	saccharides	628:638	arg1	generation					653:662	trifling generation	644:662	trifling generation of GAGs	644:670	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	3	31	theme	saccharides	628:638	arg1	existence					615:623	the general existence	603:623	the general existence of saccharides	603:638	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	1	32	theme	different	260:268	arg1	edge					292:295	mantle edge	285:295	mantle edge	285:295	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	32	theme	different	260:268	arg1	muscle					319:324	adductor muscle	310:324	adductor muscle	310:324	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	32	theme	different	260:268	arg1	parts					270:274	the different parts	256:274	the different parts	256:274	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	32	theme	different	260:268	arg1	foot					298:301	foot	298:301	foot	298:301	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	32	theme	different	260:268	arg1	gill					304:307	gill	304:307	gill	304:307	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	32	theme	different	260:268	arg1	viscera					331:337	viscera	331:337	viscera	331:337	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	edge					292:295	mantle edge	285:295	mantle edge	285:295	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	muscle					319:324	adductor muscle	310:324	adductor muscle	310:324	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	parts					270:274	the different parts	256:274	the different parts	256:274	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	foot					298:301	foot	298:301	foot	298:301	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	gill					304:307	gill	304:307	gill	304:307	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	33	from	composition	241:251	arg1	viscera					331:337	viscera	331:337	viscera	331:337	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	5	34	from	75.8 mg	883:889	arg1	10 g					894:897	10 g	894:897	10 g of 5 dried tissue materials	894:925	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	5	35	theme	dried	904:908	arg1	materials					917:925	5 dried tissue materials	902:925	5 dried tissue materials	902:925	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	2	36	theme	ingredients	365:375	arg1	occurrence					344:353	The occurrence	340:353	The occurrence of glycan ingredients	340:375	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	0	37	theme	saccharides	13:23	arg1	Abundance					0:8	Abundance	0:8	Abundance of saccharides	0:23	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	0	37	theme	saccharides	13:23	arg1	scarcity					29:36	scarcity	29:36	scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus)	29:115	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	6	38	theme	total	1142:1146	arg1	components					1155:1164	total glycan components	1142:1164	total glycan components	1142:1164	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	3	39	theme	Tissue	525:530	arg1	sections					532:539	Tissue sections	525:539	Tissue sections stained with alcian blue or periodic acid-Schiff	525:588	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	2	40	theme	glycan	358:363	arg1	ingredients					365:375	glycan ingredients	358:375	glycan ingredients	358:375	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	5	41	with	comparison	930:939	arg1	level					965:969	total carbohydrate level	946:969	total carbohydrate level in the range of 500-1760 mg	946:997	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	0	42	from	tissue	72:77	arg1	Abundance					0:8	Abundance	0:8	Abundance of saccharides	0:23	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	0	42	from	tissue	72:77	arg1	scarcity					29:36	scarcity	29:36	scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus)	29:115	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	4	43	theme	mucus	788:792	arg1	form					794:797	mucus form	788:797	mucus form	788:797	GAGs coexisting with glycogens appeared to be primarily produced in the mantle and foot tissues in mucus form by visualization.					
29983173	5	44	theme	polysaccharide	840:853	arg1	extract					855:861	the polysaccharide extract	836:861	the polysaccharide extract	836:861	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	7	45	theme	soft	1239:1242	arg1	tissues					1244:1250	The soft tissues	1235:1250	The soft tissues of clam	1235:1258	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	2	46	theme	GAG	417:419	arg1	contents					448:455	GAG and general polysaccharide contents	417:455	GAG and general polysaccharide contents in clam tissue	417:470	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	3	47	theme	general	607:613	arg1	existence					615:623	the general existence	603:623	the general existence of saccharides	603:638	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	5	48	theme	GAG	821:823	arg1	content					825:831	The GAG content	817:831	The GAG content of the polysaccharide extract	817:861	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	1	49	theme	Meretrix	192:199	arg1	tissue					215:220	Meretrix meretrix soft tissue	192:220	Meretrix meretrix soft tissue	192:220	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	2	50	from	contents	448:455	arg1	tissue					465:470	clam tissue	460:470	clam tissue	460:470	The occurrence of glycan ingredients was examined by histochemistry, whereas GAG and general polysaccharide contents in clam tissue were qualified through extraction and determination.					
29983173	7	51	theme	GAGs	1322:1325	arg1	amounts					1311:1317	sparse amounts	1304:1317	sparse amounts of GAGs	1304:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	51	theme	GAGs	1322:1325	arg1	GAGs					1322:1325	GAGs	1322:1325	GAGs	1322:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	0	52	theme	glycosaminoglycans	41:58	arg1	Abundance					0:8	Abundance	0:8	Abundance of saccharides	0:23	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	0	52	theme	glycosaminoglycans	41:58	arg1	scarcity					29:36	scarcity	29:36	scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus)	29:115	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	1	53	theme	mantle	285:290	arg1	parts					270:274	the different parts	256:274	the different parts	256:274	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	1	53	theme	mantle	285:290	arg1	edge					292:295	mantle edge	285:295	mantle edge	285:295	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	5	54	theme	polysaccharide	1062:1075	arg1	extracts					1077:1084	polysaccharide extracts	1062:1084	polysaccharide extracts	1062:1084	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	8	55	theme	M.	1435:1436	arg1	meretrix					1438:1445	M. meretrix	1435:1445	M. meretrix	1435:1445	The results will benefit the subsequent development of products made from the polysaccharide components of M. meretrix.					
29983173	5	56	theme	500-1760 mg	987:997	arg1	range					978:982	the range	974:982	the range of 500-1760 mg	974:997	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	6	57	theme	GAG	1087:1089	arg1	composition					1091:1101	GAG composition	1087:1101	GAG composition	1087:1101	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	6	58	theme	glycan	1148:1153	arg1	components					1155:1164	total glycan components	1142:1164	total glycan components	1142:1164	GAG composition only accounted for approximately 4% of total glycan components, which consist of the determinations of amino sugar and uronic acid.					
29983173	7	59	contain	contained	1260:1268	arg2	compounds					1290:1298	abundant saccharide compounds	1270:1298	abundant saccharide compounds	1270:1298	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	59	contain	contained	1260:1268	arg2	GAGs					1322:1325	GAGs	1322:1325	GAGs	1322:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	59	contain	contained	1260:1268	arg1	tissues					1244:1250	The soft tissues	1235:1250	The soft tissues of clam	1235:1258	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	7	59	contain	contained	1260:1268	arg2	amounts					1311:1317	sparse amounts	1304:1317	sparse amounts of GAGs	1304:1325	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	5	60	theme	total	946:950	arg1	level					965:969	total carbohydrate level	946:969	total carbohydrate level in the range of 500-1760 mg	946:997	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	8	61	theme	polysaccharide	1406:1419	arg1	components					1421:1430	the polysaccharide components	1402:1430	the polysaccharide components of M. meretrix	1402:1445	The results will benefit the subsequent development of products made from the polysaccharide components of M. meretrix.					
29983173	1	62	dep	Meretrix	192:199	arg1	meretrix					201:208	meretrix	201:208	meretrix	201:208	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	4	63	from	tissues	777:783	arg1	form					794:797	mucus form	788:797	mucus form	788:797	GAGs coexisting with glycogens appeared to be primarily produced in the mantle and foot tissues in mucus form by visualization.					
29983173	7	64	theme	abundant	1270:1277	arg1	compounds					1290:1298	abundant saccharide compounds	1270:1298	abundant saccharide compounds	1270:1298	The soft tissues of clam contained abundant saccharide compounds but sparse amounts of GAGs.					
29983173	1	65	theme	soft	210:213	arg1	tissue					215:220	Meretrix meretrix soft tissue	192:220	Meretrix meretrix soft tissue	192:220	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
29983173	0	66	theme	soft	67:70	arg1	tissue					72:77	the soft tissue	63:77	the soft tissue of clam	63:85	Abundance of saccharides and scarcity of glycosaminoglycans in the soft tissue of clam, Meretrix meretrix (Linnaeus).					
29983173	3	67	theme	trifling	644:651	arg1	generation					653:662	trifling generation	644:662	trifling generation of GAGs	644:670	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	3	68	theme	periodic	569:576	arg1	acid-Schiff					578:588	periodic acid-Schiff	569:588	periodic acid-Schiff	569:588	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	5	69	theme	extract	855:861	arg1	content					825:831	The GAG content	817:831	The GAG content of the polysaccharide extract	817:861	The GAG content of the polysaccharide extract ranged from 16.8 to 75.8 mg in 10 g of 5 dried tissue materials in comparison with total carbohydrate level in the range of 500-1760 mg, thereby indicating that GAGs were not the major components of polysaccharide extracts.					
29983173	3	70	theme	clam	675:678	arg1	tissues					680:686	clam tissues	675:686	clam tissues	675:686	Tissue sections stained with alcian blue or periodic acid-Schiff demonstrated the general existence of saccharides and trifling generation of GAGs in clam tissues.					
29983173	4	71	theme	foot	772:775	arg1	tissues					777:783	the mantle and foot tissues	757:783	the mantle and foot tissues in mucus form	757:797	GAGs coexisting with glycogens appeared to be primarily produced in the mantle and foot tissues in mucus form by visualization.					
29983173	1	72	from	distribution	147:158	arg1	tissue					215:220	Meretrix meretrix soft tissue	192:220	Meretrix meretrix soft tissue	192:220	We investigated presence and distribution of glycosaminoglycans (GAGs) in Meretrix meretrix soft tissue by determining GAG composition in the different parts, namely, mantle edge, foot, gill, adductor muscle, and viscera.					
30188131	10	0	contain	containing	1647:1656	arg1	emulsions					1637:1645	emulsions	1637:1645	emulsions containing only microgels	1637:1671	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	10	0	contain	containing	1647:1656	arg2	microgels					1663:1671	only microgels	1658:1671	only microgels	1658:1671	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	1	1	from	effect	192:197	arg1	capacity					208:215	their capacity to stabilize water-in-water (W/W) emulsions	202:259	their capacity to stabilize water-in-water (W/W) emulsions	202:259	Protein particles were complexed with polysaccharides, and the effect on their capacity to stabilize water-in-water (W/W) emulsions was investigated.					
30188131	6	2	theme	microgels	1014:1022	arg1	point					1001:1005	the isoionic point	988:1005	the isoionic point of the microgels (pI = 5.0)	988:1033	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	5	3	theme	microgel	738:745	arg1	concentration					747:759	a higher microgel concentration	729:759	a higher microgel concentration (3 g/L)	729:767	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	5	3	theme	microgel	738:745	arg1	g/L					764:766	3 g/L	762:766	3 g/L	762:766	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	0	4	theme	Particle	88:95	arg1	Stabilization					97:109	Influence Particle Stabilization	78:109	Influence Particle Stabilization of W/W Emulsions	78:126	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	4	5	theme	complexes	525:533	arg1	mass					502:505	molar mass	496:505	molar mass	496:505	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	4	5	theme	complexes	525:533	arg1	radius					511:516	radius	511:516	radius	511:516	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	0	6	theme	W/W	114:116	arg1	Emulsions					118:126	W/W Emulsions	114:126	W/W Emulsions	114:126	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	10	7	theme	complexation	1464:1475	arg1	function					1533:1540	a function	1531:1540	a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate	1531:1682	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	10	7	theme	complexation	1464:1475	arg1	effect					1454:1459	The effect	1450:1459	The effect of complexation on the structure of the emulsions	1450:1509	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	7	8	with	Complexation	1036:1047	arg1	polysaccharides					1054:1068	polysaccharides	1054:1068	polysaccharides	1054:1068	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	4	9	theme	molar	496:500	arg1	mass					502:505	molar mass	496:505	molar mass	496:505	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	6	10	theme	=	1028:1028	arg1	5.0					1030:1032	pI = 5.0	1025:1032	pI = 5.0	1025:1032	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	6	10	theme	=	1028:1028	arg1	microgels					1014:1022	the microgels	1010:1022	the microgels (pI = 5.0)	1010:1033	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	5	11	theme	higher	731:736	arg1	concentration					747:759	a higher microgel concentration	729:759	a higher microgel concentration (3 g/L)	729:767	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	5	11	theme	higher	731:736	arg1	g/L					764:766	3 g/L	762:766	3 g/L	762:766	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	5	12	theme	confocal	785:792	arg1	microscopy					809:818	confocal laser scanning microscopy	785:818	confocal laser scanning microscopy	785:818	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	6	13	theme	isoionic	992:999	arg1	point					1001:1005	the isoionic point	988:1005	the isoionic point of the microgels (pI = 5.0)	988:1033	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	0	14	theme	Emulsions	118:126	arg1	Stabilization					97:109	Influence Particle Stabilization	78:109	Influence Particle Stabilization of W/W Emulsions	78:126	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	3	15	dep	polysaccharides	426:440	arg1	polysaccharides					426:440	anionic or cationic polysaccharides	406:440	anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan	406:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	15	dep	polysaccharides	426:440	arg1	κ-car					458:462	κ-car	458:462	κ-car	458:462	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	15	dep	polysaccharides	426:440	arg1	chitosan					468:475	chitosan	468:475	chitosan	468:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	15	dep	polysaccharides	426:440	arg1	κ-carrageenan					443:455	κ-carrageenan	443:455	κ-carrageenan (κ-car)	443:463	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	7	16	theme	microgels	1104:1112	arg1	suspensions					1089:1099	aqueous suspensions	1081:1099	aqueous suspensions of microgels	1081:1112	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	4	17	theme	pH	654:655	arg1	function					638:645	a function	636:645	a function of the pH and the composition	636:675	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	0	18	theme	Complex	11:17	arg1	Formation					19:27	Complex Formation	11:27	Complex Formation between Polysaccharides and Protein Microgels	11:73	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	7	19	theme	polysaccharides	1171:1185	arg1	absence					1160:1166	the absence	1156:1166	the absence of polysaccharides (4.3-5.5)	1156:1195	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	10	20	theme	emulsions	1501:1509	arg1	structure					1484:1492	the structure	1480:1492	the structure of the emulsions	1480:1509	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	4	21	theme	composition	665:675	arg1	function					638:645	a function	636:645	a function of the pH and the composition	636:675	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	5	22	theme	laser	794:798	arg1	microscopy					809:818	confocal laser scanning microscopy	785:818	confocal laser scanning microscopy	785:818	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	5	23	theme	scanning	800:807	arg1	microscopy					809:818	confocal laser scanning microscopy	785:818	confocal laser scanning microscopy	785:818	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	4	24	dep	mass	502:505	arg1	The					492:494	The	492:494	The	492:494	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	10	25	theme	pH	1622:1623	arg1	range					1625:1629	the pH range	1618:1629	the pH range where emulsions containing only microgels flocculate	1618:1682	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	10	26	theme	pH.	1549:1551	arg1	function					1533:1540	a function	1531:1540	a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate	1531:1682	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	10	26	theme	pH.	1549:1551	arg1	effect					1454:1459	The effect	1450:1459	The effect of complexation on the structure of the emulsions	1450:1509	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	2	27	theme	aqueous	320:326	arg1	solutions					328:336	aqueous solutions	320:336	aqueous solutions of whey protein isolate	320:360	Protein microgels were formed by heating aqueous solutions of whey protein isolate.					
30188131	10	28	theme	liquid-like	1577:1587	arg1	emulsions					1589:1597	stable liquid-like emulsions	1570:1597	stable liquid-like emulsions	1570:1597	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	7	29	dep	they	1136:1139	arg1	flocculated					1141:1151	flocculated	1141:1151	flocculated in the absence of polysaccharides (4.3-5.5)	1141:1195	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	10	30	from	effect	1454:1459	arg1	structure					1484:1492	the structure	1480:1492	the structure of the emulsions	1480:1509	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	10	31	theme	stable	1570:1575	arg1	emulsions					1589:1597	stable liquid-like emulsions	1570:1597	stable liquid-like emulsions	1570:1597	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	7	32	theme	pH	1121:1122	arg1	range					1124:1128	the pH range	1117:1128	the pH range where they flocculated in the absence of polysaccharides (4.3-5.5)	1117:1195	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	8	33	theme	W/W	1198:1200	arg1	emulsions					1202:1210	W/W emulsions	1198:1210	W/W emulsions	1198:1210	W/W emulsions were produced by mixing dextran and poly(ethylene oxide) solutions.					
30188131	5	34	theme	complexes	709:717	arg1	structure					682:690	structure	682:690	structure	682:690	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	5	34	theme	complexes	709:717	arg1	stability					696:704	stability	696:704	stability	696:704	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	6	35	theme	stable	845:850	arg1	complexes					852:860	small stable complexes	839:860	small stable complexes	839:860	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	6	36	theme	small	839:843	arg1	complexes					852:860	small stable complexes	839:860	small stable complexes	839:860	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	7	37	theme	aqueous	1081:1087	arg1	suspensions					1089:1099	aqueous suspensions	1081:1099	aqueous suspensions of microgels	1081:1112	Complexation with polysaccharides stabilized aqueous suspensions of microgels in the pH range where they flocculated in the absence of polysaccharides (4.3-5.5).					
30188131	1	38	theme	water-in-water	230:243	arg1	emulsions					251:259	water-in-water (W/W) emulsions	230:259	water-in-water (W/W) emulsions	230:259	Protein particles were complexed with polysaccharides, and the effect on their capacity to stabilize water-in-water (W/W) emulsions was investigated.					
30188131	3	39	theme	cationic	417:424	arg1	polysaccharides					426:440	anionic or cationic polysaccharides	406:440	anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan	406:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	39	theme	cationic	417:424	arg1	chitosan					468:475	chitosan	468:475	chitosan	468:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	39	theme	cationic	417:424	arg1	κ-carrageenan					443:455	κ-carrageenan	443:455	κ-carrageenan (κ-car)	443:463	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	5	40	dep	structure	682:690	arg1	The					678:680	The	678:680	The	678:680	The structure and stability of complexes formed at a higher microgel concentration (3 g/L) were studied by confocal laser scanning microscopy.					
30188131	9	41	theme	different	1403:1411	arg1	extents					1413:1419	different extents	1403:1419	different extents	1403:1419	Microgels added to these emulsions spontaneously form a layer around the dispersed droplets, which inhibits coalescence to different extents depending on the conditions.					
30188131	1	42	theme	Protein	129:135	arg1	particles					137:145	Protein particles	129:145	Protein particles	129:145	Protein particles were complexed with polysaccharides, and the effect on their capacity to stabilize water-in-water (W/W) emulsions was investigated.					
30188131	0	43	theme	Protein	57:63	arg1	Microgels					65:73	Protein Microgels	57:73	Protein Microgels	57:73	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	4	44	theme	microgel	552:559	arg1	suspensions					561:571	dilute microgel suspensions	545:571	dilute microgel suspensions (40 mg/L)	545:581	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	4	44	theme	microgel	552:559	arg1	mg/L					577:580	40 mg/L	574:580	40 mg/L	574:580	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	2	45	theme	isolate	354:360	arg1	solutions					328:336	aqueous solutions	320:336	aqueous solutions of whey protein isolate	320:360	Protein microgels were formed by heating aqueous solutions of whey protein isolate.					
30188131	4	46	theme	dilute	545:550	arg1	suspensions					561:571	dilute microgel suspensions	545:571	dilute microgel suspensions (40 mg/L)	545:581	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	4	46	theme	dilute	545:550	arg1	mg/L					577:580	40 mg/L	574:580	40 mg/L	574:580	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	10	47	dep	pH.	1549:1551	arg1	shown					1559:1563	shown	1559:1563	is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate	1556:1682	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	2	48	theme	Protein	279:285	arg1	microgels					287:295	Protein microgels	279:295	Protein microgels	279:295	Protein microgels were formed by heating aqueous solutions of whey protein isolate.					
30188131	8	49	dep	dextran	1236:1242	arg1	solutions					1269:1277	solutions	1269:1277	solutions	1269:1277	W/W emulsions were produced by mixing dextran and poly(ethylene oxide) solutions.					
30188131	2	50	theme	protein	346:352	arg1	isolate					354:360	whey protein isolate	341:360	whey protein isolate	341:360	Protein microgels were formed by heating aqueous solutions of whey protein isolate.					
30188131	4	51	theme	scattering	611:620	arg1	techniques					622:631	light scattering techniques	605:631	light scattering techniques as a function of the pH and the composition	605:675	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	8	52	theme	ethylene	1253:1260	arg1	poly					1248:1251	poly	1248:1251	poly(ethylene oxide)	1248:1267	W/W emulsions were produced by mixing dextran and poly(ethylene oxide) solutions.					
30188131	8	52	theme	ethylene	1253:1260	arg1	oxide					1262:1266	ethylene oxide	1253:1266	ethylene oxide	1253:1266	W/W emulsions were produced by mixing dextran and poly(ethylene oxide) solutions.					
30188131	10	53	theme	only	1658:1661	arg1	microgels					1663:1671	only microgels	1658:1671	only microgels	1658:1671	The effect of complexation on the structure of the emulsions was investigated as a function of the pH. It is shown that stable liquid-like emulsions can be obtained in the pH range where emulsions containing only microgels flocculate.					
30188131	2	54	theme	whey	341:344	arg1	isolate					354:360	whey protein isolate	341:360	whey protein isolate	341:360	Protein microgels were formed by heating aqueous solutions of whey protein isolate.					
30188131	4	55	theme	light	605:609	arg1	techniques					622:631	light scattering techniques	605:631	light scattering techniques as a function of the pH and the composition	605:675	The molar mass and radius of the complexes formed in dilute microgel suspensions (40 mg/L) were characterized by light scattering techniques as a function of the pH and the composition.					
30188131	0	56	theme	Influence	78:86	arg1	Stabilization					97:109	Influence Particle Stabilization	78:109	Influence Particle Stabilization of W/W Emulsions	78:126	Exploiting Complex Formation between Polysaccharides and Protein Microgels To Influence Particle Stabilization of W/W Emulsions.					
30188131	6	57	theme	pI	1025:1026	arg1	5.0					1030:1032	pI = 5.0	1025:1032	pI = 5.0	1025:1032	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	6	57	theme	pI	1025:1026	arg1	microgels					1014:1022	the microgels	1010:1022	the microgels (pI = 5.0)	1010:1033	It was found that small stable complexes can be formed with κ-car between pH 4.3 and pH 5.5 and with chitosan between pH 4.1 and pH 6.5, that is, both below and above the isoionic point of the microgels (pI = 5.0).					
30188131	9	58	theme	dispersed	1353:1361	arg1	droplets					1363:1370	the dispersed droplets	1349:1370	the dispersed droplets	1349:1370	Microgels added to these emulsions spontaneously form a layer around the dispersed droplets, which inhibits coalescence to different extents depending on the conditions.					
30188131	3	59	theme	anionic	406:412	arg1	polysaccharides					426:440	anionic or cationic polysaccharides	406:440	anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan	406:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	59	theme	anionic	406:412	arg1	chitosan					468:475	chitosan	468:475	chitosan	468:475	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
30188131	3	59	theme	anionic	406:412	arg1	κ-carrageenan					443:455	κ-carrageenan	443:455	κ-carrageenan (κ-car)	443:463	The microgels were subsequently mixed with anionic or cationic polysaccharides: κ-carrageenan (κ-car) or chitosan, respectively.					
31306703	0	0	theme	antisolvent	84:94	arg1	method					110:115	antisolvent precipitation method	84:115	antisolvent precipitation method	84:115	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	5	1	theme	mean	658:661	arg1	size					663:666	mean size	658:666	mean size of 148 nm and PDI < 0.2	658:690	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	3	2	theme	antisolvent	523:533	arg1	method					549:554	antisolvent precipitation method	523:554	antisolvent precipitation method	523:554	Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0.					
31306703	1	3	theme	colloidal	253:261	arg1	stability					263:271	the poor colloidal stability	244:271	the poor colloidal stability of zein nanoparticles	244:293	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	6	4	from	pH 7.0	894:899	arg1	pH 4.0					872:877	pH 4.0	872:877	pH 4.0	872:877	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	1	5	theme	zein	159:162	arg1	nanoparticles					164:176	zein nanoparticles	159:176	zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS)	159:230	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	9	6	theme	cytotoxicity	1213:1224	arg1	assay					1226:1230	cytotoxicity assay	1213:1230	cytotoxicity assay on human renal epithelial cells-293 cells	1213:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	9	7	theme	zein/CS	1289:1295	arg1	nanoparticles					1307:1319	zein/CS composite nanoparticles	1289:1319	zein/CS composite nanoparticles	1289:1319	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	2	8	theme	average	300:306	arg1	weight					318:323	The average molecular weight	296:323	The average molecular weight of CS	296:329	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	8	theme	average	300:306	arg1	20.51 kDa					335:343	20.51 kDa	335:343	20.51 kDa	335:343	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	9	theme	molecular	308:316	arg1	weight					318:323	The average molecular weight	296:323	The average molecular weight of CS	296:329	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	9	theme	molecular	308:316	arg1	20.51 kDa					335:343	20.51 kDa	335:343	20.51 kDa	335:343	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	0	10	theme	precipitation	96:108	arg1	method					110:115	antisolvent precipitation method	84:115	antisolvent precipitation method	84:115	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	9	11	theme	composite	1297:1305	arg1	nanoparticles					1307:1319	zein/CS composite nanoparticles	1289:1319	zein/CS composite nanoparticles	1289:1319	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	5	12	theme	148 nm	671:676	arg1	size					663:666	mean size	658:666	mean size of 148 nm and PDI < 0.2	658:690	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	7	13	theme	regular	940:946	arg1	structures					958:967	regular spherical structures	940:967	regular spherical structures	940:967	The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions.					
31306703	1	14	theme	zein	276:279	arg1	nanoparticles					281:293	zein nanoparticles	276:293	zein nanoparticles	276:293	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	5	15	theme	Zein/CS	619:625	arg1	nanoparticles					637:649	Zein/CS composite nanoparticles	619:649	Zein/CS composite nanoparticles	619:649	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	6	16	theme	heat	799:802	arg1	treatment					804:812	heat treatment	799:812	heat treatment	799:812	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	2	17	theme	monosulfated	395:406	arg1	Di2S					421:424	monosulfated disaccharide Di2S	395:424	70.71% monosulfated disaccharide Di2S	388:424	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	6	18	from	pH 4.0	872:877	arg1	range					834:838	a range	832:838	a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	832:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	6	18	from	pH 4.0	872:877	arg1	strength					849:856	ionic strength	843:856	ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	843:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	2	19	dep	%	435:435	arg1	diS					467:469	disulfated disaccharide Di2,6 diS	437:469	29.29% disulfated disaccharide Di2,6 diS	430:469	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	8	20	theme	hydrogen	1153:1160	arg1	bonding					1162:1168	hydrogen bonding	1153:1168	hydrogen bonding	1153:1168	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	7	21	theme	zein	1009:1012	arg1	surface					998:1004	the surface	994:1004	the surface of zein	994:1012	The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions.					
31306703	2	22	theme	Di2,6	461:465	arg1	diS					467:469	disulfated disaccharide Di2,6 diS	437:469	29.29% disulfated disaccharide Di2,6 diS	430:469	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	6	23	theme	0-15 mM	861:867	arg1	strength					849:856	ionic strength	843:856	ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	843:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	2	24	theme	CS	328:329	arg1	weight					318:323	The average molecular weight	296:323	The average molecular weight of CS	296:329	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	24	theme	CS	328:329	arg1	20.51 kDa					335:343	20.51 kDa	335:343	20.51 kDa	335:343	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	7	25	theme	electrostatic	1024:1036	arg1	interactions					1038:1049	electrostatic interactions	1024:1049	electrostatic interactions	1024:1049	The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions.					
31306703	1	26	theme	anionic	183:189	arg1	CS					228:229	CS	228:229	CS	228:229	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	1	26	theme	anionic	183:189	arg1	sulfate					219:225	anionic polysaccharides-chondroitin sulfate	183:225	anionic polysaccharides-chondroitin sulfate (CS)	183:230	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	2	27	theme	disaccharide	448:459	arg1	diS					467:469	disulfated disaccharide Di2,6 diS	437:469	29.29% disulfated disaccharide Di2,6 diS	430:469	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	1	28	theme	nanoparticles	281:293	arg1	stability					263:271	the poor colloidal stability	244:271	the poor colloidal stability of zein nanoparticles	244:293	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	1	29	theme	polysaccharides-chondroitin	191:217	arg1	CS					228:229	CS	228:229	CS	228:229	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	1	29	theme	polysaccharides-chondroitin	191:217	arg1	sulfate					219:225	anionic polysaccharides-chondroitin sulfate	183:225	anionic polysaccharides-chondroitin sulfate (CS)	183:230	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	0	30	theme	zein	22:25	arg1	nanoparticles					27:39	stable zein nanoparticles	15:39	stable zein nanoparticles	15:39	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	4	31	theme	mass	579:582	arg1	ratio					584:588	The optimal mass ratio	567:588	The optimal mass ratio of zein to CS	567:602	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	4	31	theme	mass	579:582	arg1	1:1					608:610	1:1	608:610	1:1 (w/w)	608:616	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	5	32	theme	great	705:709	arg1	stability					711:719	great stability	705:719	great stability	705:719	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	7	33	theme	spherical	948:956	arg1	structures					958:967	regular spherical structures	940:967	regular spherical structures	940:967	The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions.					
31306703	8	34	theme	hydrophobic	1174:1184	arg1	interactions					1186:1197	hydrophobic interactions	1174:1197	hydrophobic interactions	1174:1197	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	3	35	theme	Zein/CS	472:478	arg1	nanoparticles					490:502	Zein/CS composite nanoparticles	472:502	Zein/CS composite nanoparticles	472:502	Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0.					
31306703	0	36	theme	stable	15:20	arg1	nanoparticles					27:39	stable zein nanoparticles	15:39	stable zein nanoparticles	15:39	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	4	37	theme	zein	593:596	arg1	ratio					584:588	The optimal mass ratio	567:588	The optimal mass ratio of zein to CS	567:602	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	4	37	theme	zein	593:596	arg1	1:1					608:610	1:1	608:610	1:1 (w/w)	608:616	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	2	38	theme	disaccharide	353:364	arg1	%					393:393	70.71%	388:393	70.71% monosulfated disaccharide Di2S	388:424	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	38	theme	disaccharide	353:364	arg1	composition					366:376	the disaccharide composition	349:376	the disaccharide composition of CS	349:382	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	3	39	theme	composite	480:488	arg1	nanoparticles					490:502	Zein/CS composite nanoparticles	472:502	Zein/CS composite nanoparticles	472:502	Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0.					
31306703	6	40	theme	strength	849:856	arg1	range					834:838	a range	832:838	a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	832:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	0	41	theme	nanoparticles	27:39	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition	0:73	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	6	42	from	range	834:838	arg1	pH 4.0					872:877	pH 4.0	872:877	pH 4.0	872:877	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	6	42	from	range	834:838	arg1	0-10 mM					883:889	0-10 mM	883:889	0-10 mM	883:889	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	6	43	theme	ionic	843:847	arg1	strength					849:856	ionic strength	843:856	ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	843:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	2	44	theme	disulfated	437:446	arg1	diS					467:469	disulfated disaccharide Di2,6 diS	437:469	29.29% disulfated disaccharide Di2,6 diS	430:469	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	45	theme	disaccharide	408:419	arg1	Di2S					421:424	monosulfated disaccharide Di2S	395:424	70.71% monosulfated disaccharide Di2S	388:424	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	0	46	theme	sulfate	56:62	arg1	deposition					64:73	chondroitin sulfate deposition	44:73	chondroitin sulfate deposition	44:73	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	9	47	from	assay	1226:1230	arg1	cells					1268:1272	human renal epithelial cells-293 cells	1235:1272	human renal epithelial cells-293 cells	1235:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	8	48	theme	urea	1084:1087	arg1	addition					1072:1079	the addition	1068:1079	the addition of urea and sodium dodecyl sulfate	1068:1114	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	8	49	theme	dodecyl	1100:1106	arg1	sulfate					1108:1114	sodium dodecyl sulfate	1093:1114	sodium dodecyl sulfate	1093:1114	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	6	50	theme	prepared	756:763	arg1	nanoparticles					765:777	these prepared nanoparticles	750:777	these prepared nanoparticles	750:777	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	0	51	theme	chondroitin	44:54	arg1	sulfate					56:62	chondroitin sulfate	44:62	chondroitin sulfate deposition	44:73	Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.					
31306703	4	52	theme	optimal	571:577	arg1	ratio					584:588	The optimal mass ratio	567:588	The optimal mass ratio of zein to CS	567:602	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	4	52	theme	optimal	571:577	arg1	1:1					608:610	1:1	608:610	1:1 (w/w)	608:616	The optimal mass ratio of zein to CS was 1:1 (w/w).					
31306703	5	53	theme	PDI < 0.2	682:690	arg1	size					663:666	mean size	658:666	mean size of 148 nm and PDI < 0.2	658:690	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	9	54	theme	human	1235:1239	arg1	cells					1268:1272	human renal epithelial cells-293 cells	1235:1272	human renal epithelial cells-293 cells	1235:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	5	55	theme	composite	627:635	arg1	nanoparticles					637:649	Zein/CS composite nanoparticles	619:649	Zein/CS composite nanoparticles	619:649	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	3	56	theme	precipitation	535:547	arg1	method					549:554	antisolvent precipitation method	523:554	antisolvent precipitation method	523:554	Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0.					
31306703	7	57	theme	composite	906:914	arg1	nanoparticles					916:928	The composite nanoparticles	902:928	The composite nanoparticles	902:928	The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions.					
31306703	1	58	with	nanoparticles	164:176	arg1	CS					228:229	CS	228:229	CS	228:229	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	1	58	with	nanoparticles	164:176	arg1	sulfate					219:225	anionic polysaccharides-chondroitin sulfate	183:225	anionic polysaccharides-chondroitin sulfate (CS)	183:230	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	9	59	theme	renal	1241:1245	arg1	cells					1268:1272	human renal epithelial cells-293 cells	1235:1272	human renal epithelial cells-293 cells	1235:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	5	60	dep	8.0	734:736	arg1	to					731:732	to	731:732	to	731:732	Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0.					
31306703	9	61	theme	epithelial	1247:1256	arg1	cells					1268:1272	human renal epithelial cells-293 cells	1235:1272	human renal epithelial cells-293 cells	1235:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	8	62	theme	sodium	1093:1098	arg1	sulfate					1108:1114	sodium dodecyl sulfate	1093:1114	sodium dodecyl sulfate	1093:1114	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	1	63	theme	work	138:141	arg1	purpose					122:128	The purpose	118:128	The purpose of this work	118:141	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
31306703	8	64	theme	sulfate	1108:1114	arg1	addition					1072:1079	the addition	1068:1079	the addition of urea and sodium dodecyl sulfate	1068:1114	In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions.					
31306703	9	65	theme	cells-293	1258:1266	arg1	cells					1268:1272	human renal epithelial cells-293 cells	1235:1272	human renal epithelial cells-293 cells	1235:1272	In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic.					
31306703	2	66	theme	CS	381:382	arg1	%					393:393	70.71%	388:393	70.71% monosulfated disaccharide Di2S	388:424	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	2	66	theme	CS	381:382	arg1	composition					366:376	the disaccharide composition	349:376	the disaccharide composition of CS	349:382	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	6	67	from	0-10 mM	883:889	arg1	range					834:838	a range	832:838	a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	832:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	6	67	from	0-10 mM	883:889	arg1	strength					849:856	ionic strength	843:856	ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0	843:899	Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0.					
31306703	2	68	dep	%	393:393	arg1	Di2S					421:424	monosulfated disaccharide Di2S	395:424	70.71% monosulfated disaccharide Di2S	388:424	The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS.					
31306703	1	69	theme	poor	248:251	arg1	stability					263:271	the poor colloidal stability	244:271	the poor colloidal stability of zein nanoparticles	244:293	The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles.					
29519322	7	0	theme	gastric	1248:1254	arg1	ulcers					1256:1261	ethanol-induced gastric ulcers	1232:1261	ethanol-induced gastric ulcers	1232:1261	It reduced ethanol-induced gastric ulcers in rats, preserving mucus and glutathione levels in the stomach.					
29519322	3	1	theme	work	503:506	arg1	objective					485:493	the objective	481:493	the objective of this work	481:506	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	5	2	theme	RSBAL	930:934	arg1	activity					883:890	The gastroprotective activity	862:890	The gastroprotective activity of the pectic polysaccharide fraction RSBAL	862:934	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	3	3	from	plant	625:629	arg1	fraction					606:613	a polysaccharide fraction	589:613	a polysaccharide fraction from this plant	589:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	3	3	from	plant	625:629	arg1	activity					577:584	the gastroprotective activity	556:584	the gastroprotective activity of a polysaccharide fraction from this plant	556:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	5	4	theme	levels	1049:1054	arg1	determination					1006:1018	determination	1006:1018	determination of the mucus and glutathione levels in the gastric tissue	1006:1076	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	6	5	theme	arabinogalactans	1203:1218	arg1	chains					1171:1176	side chains	1166:1176	side chains of arabinans and type II arabinogalactans	1166:1218	RESULTS RSBAL was constituted by a homogalacturonan and a homogalacturonan branched by side chains of arabinans and type II arabinogalactans.					
29519322	0	6	theme	dendroideum	94:104	arg1	infusion					76:83	infusion	76:83	infusion of Sedum dendroideum	76:104	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum leaves.					
29519322	5	7	theme	fraction	921:928	arg1	RSBAL					930:934	the pectic polysaccharide fraction RSBAL	895:934	the pectic polysaccharide fraction RSBAL	895:934	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	8	8	theme	dendroideum	1439:1449	arg1	activity					1424:1431	the pharmacological activity	1404:1431	the pharmacological activity of S. dendroideum	1404:1449	CONCLUSION This study demonstrated that polysaccharides could be related to the pharmacological activity of S. dendroideum.					
29519322	2	9	contain	have	339:342	arg2	properties					361:370	gastroprotective properties	344:370	gastroprotective properties	344:370	Some studies have shown that plant polysaccharides may have gastroprotective properties.					
29519322	2	9	contain	have	339:342	arg1	polysaccharides					319:333	plant polysaccharides	313:333	plant polysaccharides	313:333	Some studies have shown that plant polysaccharides may have gastroprotective properties.					
29519322	1	10	dep	Sedum	125:129	arg1	dendroideum					131:141	dendroideum	131:141	dendroideum	131:141	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	5	11	theme	gastroprotective	866:881	arg1	activity					883:890	The gastroprotective activity	862:890	The gastroprotective activity of the pectic polysaccharide fraction RSBAL	862:934	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	4	12	theme	NMR	848:850	arg1	analysis					852:859	NMR analysis	848:859	NMR analysis	848:859	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	4	13	theme	100 kDa	770:776	arg1	membrane					786:793	a 100 kDa cut-off membrane	768:793	a 100 kDa cut-off membrane	768:793	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	5	14	from	determination	1006:1018	arg1	tissue					1071:1076	the gastric tissue	1059:1076	the gastric tissue	1059:1076	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	3	15	theme	gastroprotective	435:450	arg1	activity					452:459	the gastroprotective activity	431:459	the gastroprotective activity of polysaccharides	431:478	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	6	16	theme	arabinans	1181:1189	arg1	arabinogalactans					1203:1218	arabinans and type II arabinogalactans	1181:1218	arabinans and type II arabinogalactans	1181:1218	RESULTS RSBAL was constituted by a homogalacturonan and a homogalacturonan branched by side chains of arabinans and type II arabinogalactans.					
29519322	5	17	theme	ethanol-induced	957:971	arg1	model					979:983	the ethanol-induced ulcer model	953:983	the ethanol-induced ulcer model in rats	953:991	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	3	18	theme	dendroideum	415:425	arg1	use					405:407	the popular use	393:407	the popular use of S. dendroideum	393:425	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	3	18	theme	dendroideum	415:425	arg1	activity					452:459	the gastroprotective activity	431:459	the gastroprotective activity of polysaccharides	431:478	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	0	19	theme	Gastroprotective	0:15	arg1	activity					17:24	Gastroprotective activity	0:24	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum	0:104	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum leaves.					
29519322	1	20	theme	several	253:259	arg1	problems					274:281	several other health problems	253:281	several other health problems	253:281	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	5	21	theme	ulcer	973:977	arg1	model					979:983	the ethanol-induced ulcer model	953:983	the ethanol-induced ulcer model in rats	953:991	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	5	22	from	levels	1049:1054	arg1	tissue					1071:1076	the gastric tissue	1059:1076	the gastric tissue	1059:1076	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	4	23	theme	dendroideum	662:672	arg1	Polysaccharides					640:654	METHODS Polysaccharides	632:654	METHODS Polysaccharides of S. dendroideum	632:672	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	1	24	theme	other	261:265	arg1	problems					274:281	several other health problems	253:281	several other health problems	253:281	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	3	25	from	activity	577:584	arg1	plant					625:629	this plant	620:629	this plant	620:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	3	26	theme	polysaccharides	464:478	arg1	use					405:407	the popular use	393:407	the popular use of S. dendroideum	393:425	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	3	26	theme	polysaccharides	464:478	arg1	activity					452:459	the gastroprotective activity	431:459	the gastroprotective activity of polysaccharides	431:478	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	8	27	theme	pharmacological	1408:1422	arg1	activity					1424:1431	the pharmacological activity	1404:1431	the pharmacological activity of S. dendroideum	1404:1449	CONCLUSION This study demonstrated that polysaccharides could be related to the pharmacological activity of S. dendroideum.					
29519322	1	28	theme	health	267:272	arg1	problems					274:281	several other health problems	253:281	several other health problems	253:281	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	4	29	theme	cut-off	778:784	arg1	membrane					786:793	a 100 kDa cut-off membrane	768:793	a 100 kDa cut-off membrane	768:793	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	6	30	theme	side	1166:1169	arg1	chains					1171:1176	side chains	1166:1176	side chains of arabinans and type II arabinogalactans	1166:1218	RESULTS RSBAL was constituted by a homogalacturonan and a homogalacturonan branched by side chains of arabinans and type II arabinogalactans.					
29519322	0	31	theme	polysaccharide	38:51	arg1	fraction					53:60	a pectic polysaccharide fraction	29:60	a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum	29:104	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum leaves.					
29519322	4	32	theme	monosaccharide	817:830	arg1	composition					832:842	monosaccharide composition	817:842	monosaccharide composition	817:842	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	6	33	theme	RESULTS	1079:1085	arg1	RSBAL					1087:1091	RESULTS RSBAL	1079:1091	RESULTS RSBAL	1079:1091	RESULTS RSBAL was constituted by a homogalacturonan and a homogalacturonan branched by side chains of arabinans and type II arabinogalactans.					
29519322	0	34	theme	pectic	31:36	arg1	fraction					53:60	a pectic polysaccharide fraction	29:60	a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum	29:104	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum leaves.					
29519322	5	35	theme	glutathione	1037:1047	arg1	levels					1049:1054	the mucus and glutathione levels	1023:1054	levels	1049:1054	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	1	36	theme	wound	208:212	arg1	healing					214:220	a wound healing	206:220	a wound healing agent	206:226	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	3	37	theme	popular	397:403	arg1	use					405:407	the popular use	393:407	the popular use of S. dendroideum	393:425	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	3	38	theme	gastroprotective	560:575	arg1	activity					577:584	the gastroprotective activity	556:584	the gastroprotective activity of a polysaccharide fraction from this plant	556:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	1	39	theme	Sedum	125:129	arg1	agent					222:226	a wound healing agent	206:226	a wound healing agent	206:226	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	1	39	theme	Sedum	125:129	arg1	BACKGROUND					114:123	BACKGROUND	114:123	BACKGROUND	114:123	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	0	40	theme	fraction	53:60	arg1	activity					17:24	Gastroprotective activity	0:24	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum	0:104	Gastroprotective activity of a pectic polysaccharide fraction obtained from infusion of Sedum dendroideum leaves.					
29519322	1	41	used	used	198:201	arg2	BACKGROUND					114:123	BACKGROUND	114:123	BACKGROUND	114:123	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	1	41	used	used	198:201	arg2	agent					222:226	a wound healing agent	206:226	a wound healing agent	206:226	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	2	42	theme	plant	313:317	arg1	polysaccharides					319:333	plant polysaccharides	313:333	plant polysaccharides	313:333	Some studies have shown that plant polysaccharides may have gastroprotective properties.					
29519322	5	43	from	model	979:983	arg1	rats					988:991	rats	988:991	rats	988:991	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	5	44	theme	mucus	1027:1031	arg1	levels					1049:1054	the mucus and glutathione levels	1023:1054	levels	1049:1054	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	5	45	theme	gastric	1063:1069	arg1	tissue					1071:1076	the gastric tissue	1059:1076	the gastric tissue	1059:1076	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	7	46	theme	glutathione	1293:1303	arg1	levels					1305:1310	glutathione levels	1293:1310	glutathione levels	1293:1310	It reduced ethanol-induced gastric ulcers in rats, preserving mucus and glutathione levels in the stomach.					
29519322	1	47	theme	healing	214:220	arg1	agent					222:226	a wound healing agent	206:226	a wound healing agent	206:226	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	1	47	theme	healing	214:220	arg1	BACKGROUND					114:123	BACKGROUND	114:123	BACKGROUND	114:123	BACKGROUND Sedum dendroideum, popularly known in Brazil as balsam, is traditionally used as a wound healing agent, to treat gastritis, and several other health problems.					
29519322	8	48	dep	CONCLUSION	1328:1337	arg1	demonstrated					1350:1361	demonstrated	1350:1361	demonstrated that polysaccharides could be related to the pharmacological activity of S. dendroideum	1350:1449	CONCLUSION This study demonstrated that polysaccharides could be related to the pharmacological activity of S. dendroideum.					
29519322	5	49	theme	polysaccharide	906:919	arg1	RSBAL					930:934	the pectic polysaccharide fraction RSBAL	895:934	the pectic polysaccharide fraction RSBAL	895:934	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	5	50	from	tissue	1071:1076	arg1	determination					1006:1018	determination	1006:1018	determination of the mucus and glutathione levels in the gastric tissue	1006:1076	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	4	51	theme	METHODS	632:638	arg1	Polysaccharides					640:654	METHODS Polysaccharides	632:654	METHODS Polysaccharides of S. dendroideum	632:672	METHODS Polysaccharides of S. dendroideum were extracted with water by infusion, fractionated by freeze-thawing process and dialyzed at a 100 kDa cut-off membrane, and characterized by monosaccharide composition and NMR analysis.					
29519322	3	52	theme	polysaccharide	591:604	arg1	fraction					606:613	a polysaccharide fraction	589:613	a polysaccharide fraction from this plant	589:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	5	53	theme	pectic	899:904	arg1	polysaccharide					906:919	the pectic polysaccharide	895:919	the pectic polysaccharide fraction RSBAL	895:934	The gastroprotective activity of the pectic polysaccharide fraction RSBAL was evaluated in the ethanol-induced ulcer model in rats, followed by determination of the mucus and glutathione levels in the gastric tissue.					
29519322	6	54	theme	type	1195:1198	arg1	arabinogalactans					1203:1218	arabinans and type II arabinogalactans	1181:1218	arabinans and type II arabinogalactans	1181:1218	RESULTS RSBAL was constituted by a homogalacturonan and a homogalacturonan branched by side chains of arabinans and type II arabinogalactans.					
29519322	3	55	theme	fraction	606:613	arg1	activity					577:584	the gastroprotective activity	556:584	the gastroprotective activity of a polysaccharide fraction from this plant	556:629	PURPOSE Considering the popular use of S. dendroideum and the gastroprotective activity of polysaccharides, the objective of this work was to obtain, to characterize, and to evaluate the gastroprotective activity of a polysaccharide fraction from this plant.					
29519322	2	56	theme	gastroprotective	344:359	arg1	properties					361:370	gastroprotective properties	344:370	gastroprotective properties	344:370	Some studies have shown that plant polysaccharides may have gastroprotective properties.					
29519322	7	57	theme	ethanol-induced	1232:1246	arg1	ulcers					1256:1261	ethanol-induced gastric ulcers	1232:1261	ethanol-induced gastric ulcers	1232:1261	It reduced ethanol-induced gastric ulcers in rats, preserving mucus and glutathione levels in the stomach.					
30904524	8	0	theme	IL-1β	1157:1161	arg1	levels					1113:1118	the levels	1109:1118	the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α	1109:1171	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	8	1	theme	RAW	1246:1248	arg1	cells					1267:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	9	2	theme	IL-6	1321:1324	arg1	secretion					1308:1316	the secretion	1304:1316	the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice	1304:1361	In addition ASPP could reduce the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice.					
30904524	5	3	from	rhamnose	773:780	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	0	4	theme	sweet	105:109	arg1	potato					111:116	purple sweet potato	98:116	purple sweet potato	98:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	0	5	from	characterization	11:26	arg1	potato					111:116	purple sweet potato	98:116	purple sweet potato	98:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	10	6	theme	anti-inflammation	1417:1433	arg1	agent					1435:1439	a novel anti-inflammation agent	1409:1439	a novel anti-inflammation agent	1409:1439	Our results suggest ASPP can be developed as a novel anti-inflammation agent.					
30904524	1	7	from	effect	188:193	arg1	potato					254:259	purple sweet potato	241:259	purple sweet potato	241:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	5	8	from	xylose	794:799	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	8	9	theme	TNF-α	1167:1171	arg1	levels					1113:1118	the levels	1109:1118	the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α	1109:1171	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	7	10	theme	anti-inflammation	980:996	arg1	effect					998:1003	The anti-inflammation effect	976:1003	The anti-inflammation effect of ASPP	976:1011	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	5	11	theme	Monosaccharide	707:720	arg1	analysis					736:743	Monosaccharide compositional analysis	707:743	Monosaccharide compositional analysis	707:743	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	2	12	from	polysaccharides	348:362	arg1	column					385:390	DEAE-52 cellulose column	367:390	DEAE-52 cellulose column	367:390	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	10	13	dep	suggest	1376:1382	arg1	developed					1396:1404	developed	1396:1404	suggest ASPP can be developed as a novel anti-inflammation agent	1376:1439	Our results suggest ASPP can be developed as a novel anti-inflammation agent.					
30904524	6	14	attach	attached	946:953	arg1	position					966:973	the O-6 position	958:973	the O-6 position	958:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	6	14	attach	attached	946:953	arg2	chains					939:944	side chains	934:944	side chains attached to the O-6 position	934:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	3	15	theme	main	397:400	arg1	F-1					412:414	F-1	412:414	F-1	412:414	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	3	15	theme	main	397:400	arg1	fraction					402:409	The main fraction	393:409	The main fraction (F-1)	393:415	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	5	16	theme	compositional	722:734	arg1	analysis					736:743	Monosaccharide compositional analysis	707:743	Monosaccharide compositional analysis	707:743	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	1	17	dep	characterization	149:164	arg1	the					134:136	the	134:136	the	134:136	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	9	18	theme	LPS-treated	1346:1356	arg1	mice					1358:1361	LPS-treated mice	1346:1361	LPS-treated mice	1346:1361	In addition ASPP could reduce the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice.					
30904524	6	19	theme	side	934:937	arg1	chains					939:944	side chains	934:944	side chains attached to the O-6 position	934:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	3	20	theme	sweet	497:501	arg1	ASPP					526:529	ASPP	526:529	ASPP	526:529	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	3	20	theme	sweet	497:501	arg1	polysaccharide					510:523	alkali-soluble sweet potato polysaccharide	482:523	purified alkali-soluble sweet potato polysaccharide (ASPP)	473:530	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	9	21	from	secretion	1308:1316	arg1	mice					1358:1361	LPS-treated mice	1346:1361	LPS-treated mice	1346:1361	In addition ASPP could reduce the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice.					
30904524	3	22	theme	Sephadex	441:448	arg1	column					456:461	Sephadex G-200 column	441:461	Sephadex G-200 column	441:461	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	1	23	theme	dilute	198:203	arg1	polysaccharides					220:234	dilute alkali-soluble polysaccharides	198:234	dilute alkali-soluble polysaccharides from purple sweet potato	198:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	5	24	dep	showed	745:750	arg1	composed					761:768	composed	761:768	showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3	745:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	7	25	theme	in	1054:1055	arg1	experiments					1062:1072	in vitro and in vivo experiments	1041:1072	in vitro and in vivo experiments	1041:1072	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	7	26	dep	in	1054:1055	arg1	vivo					1057:1060	vivo	1057:1060	vivo	1057:1060	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	1	27	theme	alkali-soluble	205:218	arg1	polysaccharides					220:234	dilute alkali-soluble polysaccharides	198:234	dilute alkali-soluble polysaccharides from purple sweet potato	198:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	5	28	theme	2.8:1.9:1.0:7.6:53.3	844:863	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	0	29	from	activity	50:57	arg1	potato					111:116	purple sweet potato	98:116	purple sweet potato	98:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	1	30	from	characterization	149:164	arg1	potato					254:259	purple sweet potato	241:259	purple sweet potato	241:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	2	31	theme	crude	342:346	arg1	polysaccharides					348:362	crude polysaccharides	342:362	crude polysaccharides on DEAE-52 cellulose column	342:390	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	1	32	theme	polysaccharides	220:234	arg1	characterization					149:164	structural characterization	138:164	structural characterization	138:164	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	1	32	theme	polysaccharides	220:234	arg1	effect					188:193	anti-inflammation effect	170:193	anti-inflammation effect	170:193	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	0	33	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	8	34	from	production	1190:1199	arg1	cells					1267:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	8	35	theme	-treated	1237:1244	arg1	cells					1267:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	4	36	theme	chemical	537:544	arg1	structure					546:554	The chemical structure	533:554	The chemical structure of ASPP	533:562	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	8	37	theme	macrophage	1256:1265	arg1	cells					1267:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1213:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	3	38	theme	G-200	450:454	arg1	column					456:461	Sephadex G-200 column	441:461	Sephadex G-200 column	441:461	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	0	39	theme	anti-inflammatory	32:48	arg1	activity					50:57	anti-inflammatory activity	32:57	anti-inflammatory activity	32:57	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	9	40	theme	IL-1β	1327:1331	arg1	secretion					1308:1316	the secretion	1304:1316	the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice	1304:1361	In addition ASPP could reduce the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice.					
30904524	10	41	theme	novel	1411:1415	arg1	agent					1435:1439	a novel anti-inflammation agent	1409:1439	a novel anti-inflammation agent	1409:1439	Our results suggest ASPP can be developed as a novel anti-inflammation agent.					
30904524	7	42	theme	ASPP	1008:1011	arg1	effect					998:1003	The anti-inflammation effect	976:1003	The anti-inflammation effect of ASPP	976:1011	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	1	43	theme	purple	241:246	arg1	potato					254:259	purple sweet potato	241:259	purple sweet potato	241:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	7	44	theme	in	1041:1042	arg1	experiments					1062:1072	in vitro and in vivo experiments	1041:1072	in vitro and in vivo experiments	1041:1072	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	6	45	theme	ASPP	892:895	arg1	backbone					880:887	the backbone	876:887	the backbone of ASPP	876:895	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	0	46	from	potato	111:116	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	0	46	from	potato	111:116	arg1	activity					50:57	anti-inflammatory activity	32:57	anti-inflammatory activity	32:57	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	0	46	from	potato	111:116	arg1	polysaccharides					77:91	alkali-soluble polysaccharides	62:91	alkali-soluble polysaccharides from purple sweet potato	62:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	1	47	theme	sweet	248:252	arg1	potato					254:259	purple sweet potato	241:259	purple sweet potato	241:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	4	48	theme	gas	580:582	arg1	chromatography					584:597	gas chromatography	580:597	gas chromatography	580:597	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	8	49	theme	oxide	1130:1134	arg1	levels					1113:1118	the levels	1109:1118	the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α	1109:1171	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	4	50	theme	resonance	683:691	arg1	spectroscopy					693:704	nuclear magnetic resonance spectroscopy	666:704	nuclear magnetic resonance spectroscopy	666:704	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	7	51	dep	in	1041:1042	arg1	vitro					1044:1048	vitro	1044:1048	vitro	1044:1048	The anti-inflammation effect of ASPP was further investigated by in vitro and in vivo experiments.					
30904524	2	52	dep	fractions	286:294	arg1	F-1					297:299	F-1	297:299	F-1	297:299	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	2	52	dep	fractions	286:294	arg1	fractions					286:294	Three fractions	280:294	Three fractions (F-1, F-2 and F-3)	280:313	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	2	52	dep	fractions	286:294	arg1	F-3					310:312	F-3	310:312	F-3	310:312	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	2	52	dep	fractions	286:294	arg1	F-2					302:304	F-2	302:304	F-2	302:304	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	5	53	theme	molar	829:833	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	8	54	theme	nitric	1123:1128	arg1	oxide					1130:1134	nitric oxide	1123:1134	nitric oxide	1123:1134	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	4	55	dep	Fourier	600:606	arg1	transform					608:616	transform	608:616	transform infrared spectroscopy	608:638	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	8	56	theme	IL-10	1204:1208	arg1	production					1190:1199	the production	1186:1199	the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1186:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	1	57	from	potato	254:259	arg1	polysaccharides					220:234	dilute alkali-soluble polysaccharides	198:234	dilute alkali-soluble polysaccharides from purple sweet potato	198:259	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	1	57	from	potato	254:259	arg1	characterization					149:164	structural characterization	138:164	structural characterization	138:164	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	1	57	from	potato	254:259	arg1	effect					188:193	anti-inflammation effect	170:193	anti-inflammation effect	170:193	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	3	58	theme	purified	473:480	arg1	ASPP					526:529	ASPP	526:529	ASPP	526:529	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	3	58	theme	purified	473:480	arg1	polysaccharide					510:523	alkali-soluble sweet potato polysaccharide	482:523	purified alkali-soluble sweet potato polysaccharide (ASPP)	473:530	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	0	59	theme	polysaccharides	77:91	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	0	59	theme	polysaccharides	77:91	arg1	activity					50:57	anti-inflammatory activity	32:57	anti-inflammatory activity	32:57	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	8	60	dep	showed	1083:1088	arg1	inhibit					1101:1107	inhibit	1101:1107	inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α	1101:1171	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	8	60	dep	showed	1083:1088	arg1	increase					1177:1184	increase	1177:1184	increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells	1177:1271	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	8	61	theme	interleukin	1137:1147	arg1	IL					1150:1151	interleukin (IL)-6	1137:1154	interleukin (IL)-6	1137:1154	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	6	62	theme	1,4-linked	913:922	arg1	Glcp					924:927	1,4-linked Glcp	913:927	1,4-linked Glcp with side chains attached to the O-6 position	913:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	2	63	theme	cellulose	375:383	arg1	column					385:390	DEAE-52 cellulose column	367:390	DEAE-52 cellulose column	367:390	Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column.					
30904524	0	64	theme	alkali-soluble	62:75	arg1	polysaccharides					77:91	alkali-soluble polysaccharides	62:91	alkali-soluble polysaccharides from purple sweet potato	62:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	5	65	from	glucose	814:820	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	5	66	from	arabinose	783:791	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	4	67	theme	ASPP	559:562	arg1	structure					546:554	The chemical structure	533:554	The chemical structure of ASPP	533:562	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	8	68	theme	IL	1150:1151	arg1	levels					1113:1118	the levels	1109:1118	the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α	1109:1171	Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1β and TNF-α but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells.					
30904524	9	69	theme	TNF-α	1337:1341	arg1	secretion					1308:1316	the secretion	1304:1316	the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice	1304:1361	In addition ASPP could reduce the secretion of IL-6, IL-1β and TNF-α in LPS-treated mice.					
30904524	1	70	theme	structural	138:147	arg1	characterization					149:164	structural characterization	138:164	structural characterization	138:164	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	4	71	dep	transform	608:616	arg1	infrared					618:625	infrared	618:625	transform infrared spectroscopy	608:638	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	5	72	from	mannose	802:808	arg1	ratio					835:839	the molar ratio	825:839	the molar ratio of 2.8:1.9:1.0:7.6:53.3	825:863	Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:53.3.					
30904524	6	73	link	1,4-linked	913:922	arg1	Glcp					924:927	1,4-linked Glcp	913:927	1,4-linked Glcp with side chains attached to the O-6 position	913:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	6	74	with	Glcp	924:927	arg1	chains					939:944	side chains	934:944	side chains attached to the O-6 position	934:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	3	75	theme	potato	503:508	arg1	ASPP					526:529	ASPP	526:529	ASPP	526:529	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	3	75	theme	potato	503:508	arg1	polysaccharide					510:523	alkali-soluble sweet potato polysaccharide	482:523	purified alkali-soluble sweet potato polysaccharide (ASPP)	473:530	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	0	76	theme	purple	98:103	arg1	potato					111:116	purple sweet potato	98:116	purple sweet potato	98:116	Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato.					
30904524	4	77	theme	magnetic	674:681	arg1	resonance					683:691	nuclear magnetic resonance	666:691	nuclear magnetic resonance spectroscopy	666:704	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	3	78	theme	alkali-soluble	482:495	arg1	ASPP					526:529	ASPP	526:529	ASPP	526:529	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	3	78	theme	alkali-soluble	482:495	arg1	polysaccharide					510:523	alkali-soluble sweet potato polysaccharide	482:523	purified alkali-soluble sweet potato polysaccharide (ASPP)	473:530	The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP).					
30904524	6	79	theme	O-6	962:964	arg1	position					966:973	the O-6 position	958:973	the O-6 position	958:973	Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position.					
30904524	4	80	theme	nuclear	666:672	arg1	resonance					683:691	nuclear magnetic resonance	666:691	nuclear magnetic resonance spectroscopy	666:704	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30904524	1	81	theme	anti-inflammation	170:186	arg1	effect					188:193	anti-inflammation effect	170:193	anti-inflammation effect	170:193	In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated.					
30904524	4	82	theme	methylation	641:651	arg1	analysis					653:660	methylation analysis	641:660	methylation analysis	641:660	The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy.					
30144552	2	0	theme	gut	335:337	arg1	microbiota					339:348	gut microbiota	335:348	gut microbiota	335:348	This study aimed to investigate the possible mechanism exhibited by gut microbiota in response to SCSP.					
30144552	4	1	theme	lipopolysaccharide-binding	527:552	arg1	protein					554:560	lipopolysaccharide-binding protein	527:560	lipopolysaccharide-binding protein (LBP)	527:566	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	4	1	theme	lipopolysaccharide-binding	527:552	arg1	LBP					563:565	LBP	563:565	LBP	563:565	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	9	2	used	used	1389:1392	arg2	microbiota					1465:1474	the gut microbiota	1457:1474	the gut microbiota	1457:1474	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	2	used	used	1389:1392	arg2	manipulator					1414:1424	a gut microbiota manipulator	1397:1424	a gut microbiota manipulator	1397:1424	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	2	used	used	1389:1392	arg2	SCSP					1377:1380	SCSP	1377:1380	SCSP	1377:1380	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	7	3	theme	growth	1095:1100	arg1	performance					1102:1112	the growth performance	1091:1112	the growth performance using Pearson's correlation coefficient	1091:1152	The microbial metabolites were identified to strongly correlate with the growth performance using Pearson's correlation coefficient.					
30144552	0	4	theme	normal	94:99	arg1	mice					101:104	normal mice	94:104	normal mice	94:104	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	4	5	theme	microbiota	470:479	arg1	composition					481:491	The microbiota composition	466:491	The microbiota composition	466:491	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	9	6	theme	dependent	1503:1511	arg1	manner					1513:1518	a molecular weight (Mw) dependent manner	1479:1518	a molecular weight (Mw) dependent manner	1479:1518	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	1	7	from	cucumber	140:147	arg1	polysaccharide					116:129	Sulfated polysaccharide	107:129	Sulfated polysaccharide from sea cucumber (SCSP)	107:154	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	8	8	theme	growth	1320:1325	arg1	performance					1327:1337	related growth performance	1312:1337	related growth performance	1312:1337	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	8	9	theme	related	1312:1318	arg1	performance					1327:1337	related growth performance	1312:1337	related growth performance	1312:1337	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	5	10	theme	gut	685:687	arg1	microbiota					689:698	the gut microbiota	681:698	the gut microbiota	681:698	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	9	11	theme	gut	1461:1463	arg1	SCSP					1377:1380	SCSP	1377:1380	SCSP	1377:1380	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	11	theme	gut	1461:1463	arg1	microbiota					1465:1474	the gut microbiota	1457:1474	the gut microbiota	1457:1474	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	11	theme	gut	1461:1463	arg1	manipulator					1414:1424	a gut microbiota manipulator	1397:1424	a gut microbiota manipulator	1397:1424	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	6	12	theme	tissue	1008:1013	arg1	index					1015:1019	gut tissue index	1004:1019	gut tissue index	1004:1019	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	5	13	theme	harmful	821:827	arg1	bacteria					829:836	harmful bacteria	821:836	harmful bacteria	821:836	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	9	14	theme	gut	1399:1401	arg1	manipulator					1414:1424	a gut microbiota manipulator	1397:1424	a gut microbiota manipulator	1397:1424	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	14	theme	gut	1399:1401	arg1	microbiota					1465:1474	the gut microbiota	1457:1474	the gut microbiota	1457:1474	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	14	theme	gut	1399:1401	arg1	SCSP					1377:1380	SCSP	1377:1380	SCSP	1377:1380	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	8	15	from	effect	1193:1198	arg1	microbiota					1219:1228	the gut microbiota	1211:1228	the gut microbiota	1211:1228	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	5	16	theme	SCFA-producing	751:764	arg1	diversity					740:748	the enriched microbiota diversity	716:748	the enriched microbiota diversity	716:748	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	16	theme	SCFA-producing	751:764	arg1	bacteria					766:773	SCFA-producing bacteria	751:773	SCFA-producing bacteria	751:773	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	0	17	from	cucumber	33:40	arg1	polysaccharide					9:22	Sulfated polysaccharide	0:22	Sulfated polysaccharide from sea cucumber	0:40	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	0	18	theme	Sulfated	0:7	arg1	polysaccharide					9:22	Sulfated polysaccharide	0:22	Sulfated polysaccharide from sea cucumber	0:40	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	8	19	theme	gut	1215:1217	arg1	microbiota					1219:1228	the gut microbiota	1211:1228	the gut microbiota	1211:1228	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	0	20	theme	sea	29:31	arg1	cucumber					33:40	sea cucumber	29:40	sea cucumber	29:40	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	7	21	theme	microbial	1026:1034	arg1	metabolites					1036:1046	The microbial metabolites	1022:1046	The microbial metabolites	1022:1046	The microbial metabolites were identified to strongly correlate with the growth performance using Pearson's correlation coefficient.					
30144552	9	22	theme	microbiota	1403:1412	arg1	manipulator					1414:1424	a gut microbiota manipulator	1397:1424	a gut microbiota manipulator	1397:1424	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	22	theme	microbiota	1403:1412	arg1	microbiota					1465:1474	the gut microbiota	1457:1474	the gut microbiota	1457:1474	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	22	theme	microbiota	1403:1412	arg1	SCSP					1377:1380	SCSP	1377:1380	SCSP	1377:1380	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	6	23	theme	gut	1004:1006	arg1	index					1015:1019	gut tissue index	1004:1019	gut tissue index	1004:1019	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	1	24	theme	various	183:189	arg1	effects					198:204	various health effects	183:204	various health effects	183:204	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	5	25	theme	sulfide-degrading	779:795	arg1	diversity					740:748	the enriched microbiota diversity	716:748	the enriched microbiota diversity	716:748	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	25	theme	sulfide-degrading	779:795	arg1	bacteria					797:804	sulfide-degrading bacteria	779:804	sulfide-degrading bacteria	779:804	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	0	26	from	metabolites	79:89	arg1	mice					101:104	normal mice	94:104	normal mice	94:104	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	0	27	dep	microbiota	60:69	arg1	the					52:54	the	52:54	the	52:54	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	1	28	theme	health	191:196	arg1	effects					198:204	various health effects	183:204	various health effects	183:204	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	5	29	theme	enriched	720:727	arg1	diversity					740:748	the enriched microbiota diversity	716:748	the enriched microbiota diversity	716:748	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	29	theme	enriched	720:727	arg1	bacteria					766:773	SCFA-producing bacteria	751:773	SCFA-producing bacteria	751:773	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	29	theme	enriched	720:727	arg1	bacteria					797:804	sulfide-degrading bacteria	779:804	sulfide-degrading bacteria	779:804	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	6	30	theme	metabolites	921:931	arg1	levels					901:906	the levels	897:906	the levels of microbial metabolites including SCFAs and LBP	897:955	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	1	31	theme	effects	198:204	arg1	mechanism					211:219	the mechanism	207:219	the mechanism	207:219	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	9	32	theme	health	1430:1435	arg1	promotion					1437:1445	health promotion	1430:1445	health promotion	1430:1445	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	4	33	theme	tissue	589:594	arg1	index					596:600	gut tissue index	585:600	gut tissue index	585:600	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	5	34	theme	microbiota	729:738	arg1	diversity					740:748	the enriched microbiota diversity	716:748	the enriched microbiota diversity	716:748	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	34	theme	microbiota	729:738	arg1	bacteria					766:773	SCFA-producing bacteria	751:773	SCFA-producing bacteria	751:773	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	5	34	theme	microbiota	729:738	arg1	bacteria					797:804	sulfide-degrading bacteria	779:804	sulfide-degrading bacteria	779:804	Results revealed that both SCSP and d-SCSP positively regulated the gut microbiota as indicated by the enriched microbiota diversity, SCFA-producing bacteria and sulfide-degrading bacteria, and decreased harmful bacteria.					
30144552	4	35	theme	short	494:498	arg1	SCFAs					519:523	SCFAs	519:523	SCFAs	519:523	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	4	35	theme	short	494:498	arg1	acids					512:516	short chain fatty acids	494:516	short chain fatty acids (SCFAs)	494:524	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	8	36	theme	modulating	1182:1191	arg1	effect					1193:1198	the modulating effect	1178:1198	the modulating effect of SCSP on the gut microbiota	1178:1228	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	8	37	theme	free-radical	1245:1256	arg1	depolymerization					1258:1273	free-radical depolymerization	1245:1273	free-radical depolymerization	1245:1273	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	7	38	theme	correlation	1130:1140	arg1	coefficient					1142:1152	Pearson's correlation coefficient	1120:1152	Pearson's correlation coefficient	1120:1152	The microbial metabolites were identified to strongly correlate with the growth performance using Pearson's correlation coefficient.					
30144552	4	39	theme	gut	585:587	arg1	index					596:600	gut tissue index	585:600	gut tissue index	585:600	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	0	40	theme	gut	56:58	arg1	microbiota					60:69	gut microbiota	56:69	gut microbiota	56:69	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	6	41	theme	microbial	911:919	arg1	LBP					953:955	LBP	953:955	LBP	953:955	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	6	41	theme	microbial	911:919	arg1	SCFAs					943:947	SCFAs	943:947	SCFAs	943:947	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	6	41	theme	microbial	911:919	arg1	metabolites					921:931	microbial metabolites	911:931	microbial metabolites including SCFAs and LBP	911:955	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	0	42	from	microbiota	60:69	arg1	mice					101:104	normal mice	94:104	normal mice	94:104	Sulfated polysaccharide from sea cucumber modulates the gut microbiota and its metabolites in normal mice.					
30144552	1	43	theme	Sulfated	107:114	arg1	polysaccharide					116:129	Sulfated polysaccharide	107:129	Sulfated polysaccharide from sea cucumber (SCSP)	107:154	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	4	44	theme	fatty	506:510	arg1	SCFAs					519:523	SCFAs	519:523	SCFAs	519:523	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	4	44	theme	fatty	506:510	arg1	acids					512:516	short chain fatty acids	494:516	short chain fatty acids (SCFAs)	494:524	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	4	45	theme	chain	500:504	arg1	SCFAs					519:523	SCFAs	519:523	SCFAs	519:523	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	4	45	theme	chain	500:504	arg1	acids					512:516	short chain fatty acids	494:516	short chain fatty acids (SCFAs)	494:524	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	2	46	theme	possible	303:310	arg1	mechanism					312:320	the possible mechanism	299:320	the possible mechanism exhibited by gut microbiota in response to SCSP	299:368	This study aimed to investigate the possible mechanism exhibited by gut microbiota in response to SCSP.					
30144552	1	47	theme	sea	136:138	arg1	SCSP					150:153	SCSP	150:153	SCSP	150:153	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	1	47	theme	sea	136:138	arg1	cucumber					140:147	sea cucumber	136:147	sea cucumber (SCSP)	136:154	Sulfated polysaccharide from sea cucumber (SCSP) has been demonstrated with various health effects, the mechanism of which, however, remains poorly understood.					
30144552	8	48	theme	SCSP	1203:1206	arg1	effect					1193:1198	the modulating effect	1178:1198	the modulating effect of SCSP on the gut microbiota	1178:1228	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
30144552	6	49	theme	body	988:991	arg1	weight					993:998	body weight	988:998	body weight	988:998	Moreover, SCSP and d-SCSP not only significantly improved the levels of microbial metabolites including SCFAs and LBP, but also effectively adjusted body weight and gut tissue index.					
30144552	3	50	theme	depolymerized	425:437	arg1	d-SCSP					445:450	d-SCSP	445:450	d-SCSP	445:450	BALB/c mice were fed diets supplemented with SCSP and depolymerized SCSP (d-SCSP) for 42 days.					
30144552	3	50	theme	depolymerized	425:437	arg1	SCSP					439:442	depolymerized SCSP	425:442	depolymerized SCSP (d-SCSP)	425:451	BALB/c mice were fed diets supplemented with SCSP and depolymerized SCSP (d-SCSP) for 42 days.					
30144552	9	51	theme	molecular	1481:1489	arg1	weight					1491:1496	molecular weight	1481:1496	molecular weight (Mw)	1481:1501	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	9	51	theme	molecular	1481:1489	arg1	Mw					1499:1500	Mw	1499:1500	Mw	1499:1500	These findings suggest that SCSP can be used as a gut microbiota manipulator for health promotion and alter the gut microbiota in a molecular weight (Mw) dependent manner.					
30144552	4	52	theme	body	569:572	arg1	weight					574:579	body weight	569:579	body weight	569:579	The microbiota composition, short chain fatty acids (SCFAs), lipopolysaccharide-binding protein (LBP), body weight and gut tissue index were analyzed.					
30144552	3	53	theme	BALB/c	371:376	arg1	mice					378:381	BALB/c mice	371:381	BALB/c mice	371:381	BALB/c mice were fed diets supplemented with SCSP and depolymerized SCSP (d-SCSP) for 42 days.					
30144552	2	54	dep	SCSP	365:368	arg1	response					353:360	response	353:360	response	353:360	This study aimed to investigate the possible mechanism exhibited by gut microbiota in response to SCSP.					
30144552	8	55	theme	microbial	1286:1294	arg1	metabolites					1296:1306	the microbial metabolites	1282:1306	the microbial metabolites	1282:1306	We further showed that the modulating effect of SCSP on the gut microbiota was altered by free-radical depolymerization, while the microbial metabolites and related growth performance were not.					
31769514	8	0	from	applicability	1183:1195	arg1	formulations					1213:1224	food formulations	1208:1224	food formulations	1208:1224	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	10	1	theme	low	1368:1370	arg1	content					1380:1386	low protein content	1368:1386	low protein content	1368:1386	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	1	2	theme	Kexin	249:253	arg1	cultivars					260:268	Kexin No.1 cultivars	249:268	Kexin No.1 cultivars	249:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	8	3	theme	potato	1153:1158	arg1	flours					1160:1165	potato flours	1153:1165	potato flours	1153:1165	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	6	4	theme	bulk	869:872	arg1	density					874:880	bulk density	869:880	bulk density of them	869:888	However, bulk density of them slightly reduced with the increase in fermentation time.					
31769514	11	5	theme	flour	1625:1629	arg1	characteristics					1570:1584	the nutritional and functional characteristics	1539:1584	the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF)	1539:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	10	6	theme	potato	1391:1396	arg1	flours					1398:1403	potato flours	1391:1403	potato flours	1391:1403	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	3	7	theme	ash	514:516	arg1	contents					518:525	increased protein and ash contents	492:525	increased protein and ash contents	492:525	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	5	8	theme	potato	825:830	arg1	flours					832:837	potato flours	825:837	potato flours during fermentation	825:857	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	12	9	theme	study	1684:1688	arg1	findings					1667:1674	findings	1667:1674	findings of this study	1667:1688	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	6	10	theme	fermentation	928:939	arg1	time					941:944	fermentation time	928:944	fermentation time	928:944	However, bulk density of them slightly reduced with the increase in fermentation time.					
31769514	11	11	theme	yeast	1511:1515	arg1	fermentation					1517:1528	yeast fermentation	1511:1528	yeast fermentation	1511:1528	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	7	12	theme	flours	1046:1051	arg1	stability					1014:1022	freeze-thaw stability	1002:1022	freeze-thaw stability of gelatinized potato flours	1002:1051	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	7	13	theme	gelatinized	1027:1037	arg1	flours					1046:1051	gelatinized potato flours	1027:1051	gelatinized potato flours	1027:1051	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	3	14	theme	lipid	566:570	arg1	levels					546:551	the levels	542:551	the levels of moisture, lipid, soluble amylose, amylopectin, and total starch	542:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	1	15	theme	gelatinized	205:215	arg1	flours					224:229	gelatinized potato flours	205:229	gelatinized potato flours from Atlantic and Kexin No.1 cultivars	205:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	16	from	Atlantic	236:243	arg1	properties					191:200	functional properties	180:200	functional properties	180:200	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	16	from	Atlantic	236:243	arg1	flours					224:229	gelatinized potato flours	205:229	gelatinized potato flours from Atlantic and Kexin No.1 cultivars	205:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	16	from	Atlantic	236:243	arg1	color					169:173	color	169:173	color	169:173	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	16	from	Atlantic	236:243	arg1	compositions					155:166	compositions	155:166	compositions	155:166	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	3	17	theme	moisture	556:563	arg1	levels					546:551	the levels	542:551	the levels of moisture, lipid, soluble amylose, amylopectin, and total starch	542:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	5	18	theme	emulsifying	775:785	arg1	capacity					787:794	emulsifying capacity	775:794	emulsifying capacity	775:794	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	1	19	theme	fermentation	139:150	arg1	effects					128:134	effects	128:134	effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars	128:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	4	20	theme	potato	652:657	arg1	flours					659:664	potato flours	652:664	potato flours	652:664	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	0	21	from	Effects	0:6	arg1	Properties					63:72	Functional Properties	52:72	Functional Properties	52:72	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	21	from	Effects	0:6	arg1	Color					41:45	Color	41:45	Color	41:45	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	21	from	Effects	0:6	arg1	Compositions					27:38	Compositions	27:38	Compositions	27:38	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	22	theme	Gelatinized	77:87	arg1	Flours					96:101	Gelatinized Potato Flours	77:101	Gelatinized Potato Flours	77:101	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	23	theme	Flours	96:101	arg1	Properties					63:72	Functional Properties	52:72	Functional Properties	52:72	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	23	theme	Flours	96:101	arg1	Color					41:45	Color	41:45	Color	41:45	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	0	23	theme	Flours	96:101	arg1	Compositions					27:38	Compositions	27:38	Compositions	27:38	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	10	24	theme	food	1427:1430	arg1	formulations					1432:1443	food formulations	1427:1443	food formulations	1427:1443	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	2	25	theme	%	339:339	arg1	concentration					347:359	1% yeast concentration	338:359	1% yeast concentration	338:359	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	9	26	theme	flour	1321:1325	arg1	use					1307:1309	the use	1303:1309	the use of potato flour in foods	1303:1334	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	4	27	from	increase	689:696	arg1	time					714:717	fermentation time	701:717	fermentation time	701:717	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	4	28	theme	fermentation	701:712	arg1	time					714:717	fermentation time	701:717	fermentation time	701:717	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	0	29	theme	Fermentation	11:22	arg1	Effects					0:6	Effects	0:6	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours	0:101	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	3	30	theme	total	607:611	arg1	starch					613:618	total starch	607:618	total starch	607:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	11	31	theme	Atlantic	1589:1596	arg1	AF					1605:1606	AF	1605:1606	AF	1605:1606	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	11	31	theme	Atlantic	1589:1596	arg1	flour					1598:1602	Atlantic flour	1589:1602	Atlantic flour (AF)	1589:1607	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	3	32	theme	flours	473:478	arg1	features					442:449	the nutritional and physicochemical features	406:449	the nutritional and physicochemical features of gelatinized potato flours	406:478	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	5	33	theme	water	751:755	arg1	index					768:772	water absorption index	751:772	water absorption index	751:772	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	3	34	theme	soluble	573:579	arg1	amylose					581:587	soluble amylose	573:587	soluble amylose	573:587	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	11	35	theme	nutritional	1543:1553	arg1	characteristics					1570:1584	the nutritional and functional characteristics	1539:1584	the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF)	1539:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	3	36	theme	gelatinized	454:464	arg1	flours					473:478	gelatinized potato flours	454:478	gelatinized potato flours	454:478	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	1	37	theme	potato	217:222	arg1	flours					224:229	gelatinized potato flours	205:229	gelatinized potato flours from Atlantic and Kexin No.1 cultivars	205:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	12	38	theme	flours	1741:1746	arg1	selection					1721:1729	the selection	1717:1729	the selection of potato flours for various food formulations	1717:1776	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	3	39	theme	increased	492:500	arg1	contents					518:525	increased protein and ash contents	492:525	increased protein and ash contents	492:525	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	6	40	theme	them	885:888	arg1	density					874:880	bulk density	869:880	bulk density of them	869:888	However, bulk density of them slightly reduced with the increase in fermentation time.					
31769514	12	41	theme	various	1752:1758	arg1	formulations					1765:1776	various food formulations	1752:1776	various food formulations	1752:1776	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	8	42	theme	processing	1123:1132	arg1	characteristics					1134:1148	certain processing characteristics	1115:1148	certain processing characteristics of potato flours	1115:1165	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	7	43	contain	has	973:975	arg2	effect					992:997	no significant effect	977:997	no significant effect	977:997	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	7	43	contain	has	973:975	arg1	fermentation					960:971	fermentation	960:971	fermentation	960:971	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	11	44	theme	Kexin	1613:1617	arg1	KF					1632:1633	KF	1632:1633	KF	1632:1633	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	11	44	theme	Kexin	1613:1617	arg1	flour					1625:1629	Kexin No. 1 flour	1613:1629	Kexin No. 1 flour (KF)	1613:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	1	45	theme	No.1	255:258	arg1	cultivars					260:268	Kexin No.1 cultivars	249:268	Kexin No.1 cultivars	249:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	10	46	theme	dark	1353:1356	arg1	color					1358:1362	dark color	1353:1362	dark color	1353:1362	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	2	47	theme	Atlantic	271:278	arg1	flour					280:284	Atlantic flour	271:284	Atlantic flour (AF)	271:289	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	2	47	theme	Atlantic	271:278	arg1	AF					287:288	AF	287:288	AF	287:288	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	8	48	theme	flours	1160:1165	arg1	characteristics					1134:1148	certain processing characteristics	1115:1148	certain processing characteristics of potato flours	1115:1165	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	2	49	theme	Kexin	295:299	arg1	KF					313:314	KF	313:314	KF	313:314	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	2	49	theme	Kexin	295:299	arg1	flour					306:310	Kexin No.1 flour	295:310	Kexin No.1 flour (KF)	295:315	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	5	50	theme	flours	832:837	arg1	index					768:772	water absorption index	751:772	water absorption index	751:772	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	5	50	theme	flours	832:837	arg1	capacity					787:794	emulsifying capacity	775:794	emulsifying capacity	775:794	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	5	50	theme	flours	832:837	arg1	stability					812:820	emulsifying stability	800:820	emulsifying stability	800:820	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	7	51	theme	potato	1039:1044	arg1	flours					1046:1051	gelatinized potato flours	1027:1051	gelatinized potato flours	1027:1051	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	10	52	theme	protein	1372:1378	arg1	content					1380:1386	low protein content	1368:1386	low protein content	1368:1386	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	1	53	theme	functional	180:189	arg1	properties					191:200	functional properties	180:200	functional properties	180:200	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	9	54	from	use	1307:1309	arg1	foods					1330:1334	foods	1330:1334	foods	1330:1334	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	10	55	from	application	1412:1422	arg1	formulations					1432:1443	food formulations	1427:1443	food formulations	1427:1443	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	9	56	theme	PRACTICAL	1227:1235	arg1	APPLICATION					1237:1247	PRACTICAL APPLICATION	1227:1247	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.	1227:1335	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	5	57	theme	emulsifying	800:810	arg1	stability					812:820	emulsifying stability	800:820	emulsifying stability	800:820	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	8	58	theme	them	1200:1203	arg1	applicability					1183:1195	the applicability	1179:1195	the applicability of them in food formulations	1179:1224	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	7	59	theme	freeze-thaw	1002:1012	arg1	stability					1014:1022	freeze-thaw stability	1002:1022	freeze-thaw stability of gelatinized potato flours	1002:1051	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	7	60	theme	significant	980:990	arg1	effect					992:997	no significant effect	977:997	no significant effect	977:997	In addition, fermentation has no significant effect on freeze-thaw stability of gelatinized potato flours.					
31769514	8	61	theme	food	1208:1211	arg1	formulations					1213:1224	food formulations	1208:1224	food formulations	1208:1224	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	4	62	theme	flours	659:664	arg1	whiteness					639:647	whiteness	639:647	whiteness	639:647	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	4	62	theme	flours	659:664	arg1	lightness					625:633	lightness	625:633	lightness	625:633	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	3	63	theme	amylose	581:587	arg1	levels					546:551	the levels	542:551	the levels of moisture, lipid, soluble amylose, amylopectin, and total starch	542:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	1	64	from	properties	191:200	arg1	Atlantic					236:243	Atlantic	236:243	Atlantic	236:243	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	64	from	properties	191:200	arg1	cultivars					260:268	Kexin No.1 cultivars	249:268	Kexin No.1 cultivars	249:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	0	65	theme	Functional	52:61	arg1	Properties					63:72	Functional Properties	52:72	Functional Properties	52:72	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	11	66	theme	possible	1482:1489	arg1	mechanisms					1491:1500	the possible mechanisms	1478:1500	the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF)	1478:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	9	67	theme	national	1276:1283	arg1	project					1285:1291	national project	1276:1291	national project regarding the use of potato flour in foods	1276:1334	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	1	68	from	color	169:173	arg1	Atlantic					236:243	Atlantic	236:243	Atlantic	236:243	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	68	from	color	169:173	arg1	cultivars					260:268	Kexin No.1 cultivars	249:268	Kexin No.1 cultivars	249:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	69	from	cultivars	260:268	arg1	properties					191:200	functional properties	180:200	functional properties	180:200	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	69	from	cultivars	260:268	arg1	flours					224:229	gelatinized potato flours	205:229	gelatinized potato flours from Atlantic and Kexin No.1 cultivars	205:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	69	from	cultivars	260:268	arg1	color					169:173	color	169:173	color	169:173	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	69	from	cultivars	260:268	arg1	compositions					155:166	compositions	155:166	compositions	155:166	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	0	70	theme	Potato	89:94	arg1	Flours					96:101	Gelatinized Potato Flours	77:101	Gelatinized Potato Flours	77:101	Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.					
31769514	5	71	from	increases	738:746	arg1	index					768:772	water absorption index	751:772	water absorption index	751:772	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	5	71	from	increases	738:746	arg1	capacity					787:794	emulsifying capacity	775:794	emulsifying capacity	775:794	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	5	71	from	increases	738:746	arg1	stability					812:820	emulsifying stability	800:820	emulsifying stability	800:820	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	1	72	from	compositions	155:166	arg1	Atlantic					236:243	Atlantic	236:243	Atlantic	236:243	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	72	from	compositions	155:166	arg1	cultivars					260:268	Kexin No.1 cultivars	249:268	Kexin No.1 cultivars	249:268	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	12	73	theme	applications	1642:1653	arg1	standpoint					1655:1664	applications standpoint	1642:1664	applications standpoint	1642:1664	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	10	74	theme	flours	1398:1403	arg1	content					1380:1386	low protein content	1368:1386	low protein content	1368:1386	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	10	74	theme	flours	1398:1403	arg1	color					1358:1362	dark color	1353:1362	dark color	1353:1362	However, due to dark color and low protein content of potato flours, their application in food formulations was limited.					
31769514	2	75	theme	No.1	301:304	arg1	KF					313:314	KF	313:314	KF	313:314	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	2	75	theme	No.1	301:304	arg1	flour					306:310	Kexin No.1 flour	295:310	Kexin No.1 flour (KF)	295:315	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	2	76	theme	yeast	341:345	arg1	concentration					347:359	1% yeast concentration	338:359	1% yeast concentration	338:359	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	5	77	theme	absorption	757:766	arg1	index					768:772	water absorption index	751:772	water absorption index	751:772	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	9	78	theme	potato	1314:1319	arg1	flour					1321:1325	potato flour	1314:1325	potato flour	1314:1325	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	1	79	from	effects	128:134	arg1	properties					191:200	functional properties	180:200	functional properties	180:200	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	79	from	effects	128:134	arg1	color					169:173	color	169:173	color	169:173	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	79	from	effects	128:134	arg1	compositions					155:166	compositions	155:166	compositions	155:166	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	2	80	theme	1	338:338	arg1	%					339:339	%	339:339	%	339:339	Atlantic flour (AF) and Kexin No.1 flour (KF) were fermented using 1% yeast concentration, respectively.					
31769514	12	81	from	knowledge	1704:1712	arg1	selection					1721:1729	the selection	1717:1729	the selection of potato flours for various food formulations	1717:1776	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	6	82	from	increase	916:923	arg1	time					941:944	fermentation time	928:944	fermentation time	928:944	However, bulk density of them slightly reduced with the increase in fermentation time.					
31769514	5	83	theme	gradual	730:736	arg1	increases					738:746	gradual increases	730:746	gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation	730:857	There are gradual increases in water absorption index, emulsifying capacity and emulsifying stability of potato flours during fermentation.					
31769514	3	84	theme	amylopectin	590:600	arg1	levels					546:551	the levels	542:551	the levels of moisture, lipid, soluble amylose, amylopectin, and total starch	542:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	3	85	theme	physicochemical	426:440	arg1	features					442:449	the nutritional and physicochemical features	406:449	the nutritional and physicochemical features of gelatinized potato flours	406:478	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	8	86	theme	yeast	1082:1086	arg1	fermentation					1088:1099	yeast fermentation	1082:1099	yeast fermentation	1082:1099	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	12	87	theme	food	1760:1763	arg1	formulations					1765:1776	various food formulations	1752:1776	various food formulations	1752:1776	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	11	88	theme	functional	1559:1568	arg1	characteristics					1570:1584	the nutritional and functional characteristics	1539:1584	the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF)	1539:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	4	89	dep	lightness	625:633	arg1	The					621:623	The	621:623	The	621:623	The lightness and whiteness of potato flours were enhanced with the increase in fermentation time.					
31769514	9	90	dep	APPLICATION	1237:1247	arg1	started					1268:1274	started	1268:1274	has started national project regarding the use of potato flour in foods	1264:1334	PRACTICAL APPLICATION: Lately, China has started national project regarding the use of potato flour in foods.					
31769514	3	91	theme	starch	613:618	arg1	levels					546:551	the levels	542:551	the levels of moisture, lipid, soluble amylose, amylopectin, and total starch	542:618	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	3	92	theme	potato	466:471	arg1	flours					473:478	gelatinized potato flours	454:478	gelatinized potato flours	454:478	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	1	93	theme	flours	224:229	arg1	properties					191:200	functional properties	180:200	functional properties	180:200	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	93	theme	flours	224:229	arg1	color					169:173	color	169:173	color	169:173	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	1	93	theme	flours	224:229	arg1	compositions					155:166	compositions	155:166	compositions	155:166	This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars.					
31769514	8	94	theme	certain	1115:1121	arg1	characteristics					1134:1148	certain processing characteristics	1115:1148	certain processing characteristics of potato flours	1115:1165	These results indicate that yeast fermentation could enhance certain processing characteristics of potato flours and improve the applicability of them in food formulations.					
31769514	12	95	theme	potato	1734:1739	arg1	flours					1741:1746	potato flours	1734:1746	potato flours	1734:1746	From applications standpoint, findings of this study could provide knowledge on the selection of potato flours for various food formulations.					
31769514	3	96	theme	nutritional	410:420	arg1	features					442:449	the nutritional and physicochemical features	406:449	the nutritional and physicochemical features of gelatinized potato flours	406:478	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
31769514	11	97	theme	No.	1619:1621	arg1	KF					1632:1633	KF	1632:1633	KF	1632:1633	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	11	97	theme	No.	1619:1621	arg1	flour					1625:1629	Kexin No. 1 flour	1613:1629	Kexin No. 1 flour (KF)	1613:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	11	98	theme	flour	1598:1602	arg1	characteristics					1570:1584	the nutritional and functional characteristics	1539:1584	the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF)	1539:1634	This study analyzed the possible mechanisms by which yeast fermentation improved the nutritional and functional characteristics of Atlantic flour (AF) and Kexin No. 1 flour (KF).					
31769514	3	99	theme	protein	502:508	arg1	contents					518:525	increased protein and ash contents	492:525	increased protein and ash contents	492:525	Fermentation further improved the nutritional and physicochemical features of gelatinized potato flours by means of increased protein and ash contents, and decreased the levels of moisture, lipid, soluble amylose, amylopectin, and total starch.					
29307227	0	0	theme	pasta	81:85	arg1	features					101:108	pasta technological features	81:108	pasta technological features	81:108	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	5	1	theme	developed	1020:1028	arg1	structure					1030:1038	a better developed structure	1011:1038	a better developed structure	1011:1038	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	2	with	design	393:398	arg1	methodology					433:443	randomised response surface methodology	405:443	randomised response surface methodology	405:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	3	theme	bran-enriched	986:998	arg1	pasta					1000:1004	bran-enriched pasta	986:1004	bran-enriched pasta with a better developed structure and superior cooking behaviour	986:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	1	4	theme	appropriate	234:244	arg1	properties					264:273	appropriate techno-functional properties	234:273	appropriate techno-functional properties	234:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	3	5	theme	swelling	634:641	arg1	index					643:647	swelling index	634:647	swelling index	634:647	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	1	6	theme	fibre-enriched	146:159	arg1	pasta					167:171	fibre-enriched fresh pasta	146:171	fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties	146:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	1	7	theme	techno-functional	246:262	arg1	properties					264:273	appropriate techno-functional properties	234:273	appropriate techno-functional properties	234:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	4	8	theme	uncooked	832:839	arg1	pasta					841:845	uncooked pasta	832:845	uncooked pasta	832:845	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	5	9	theme	xanthan	947:953	arg1	gum					955:957	xanthan gum	947:957	: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour	941:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	10	theme	xanthan	1094:1100	arg1	gum					1102:1104	xanthan gum	1094:1104	xanthan gum (0.8% w/w)	1094:1115	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	10	theme	xanthan	1094:1100	arg1	%					1110:1110	0.8% w/w	1107:1114	0.8% w/w	1107:1114	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	0	11	theme	technological	87:99	arg1	features					101:108	pasta technological features	81:108	pasta technological features	81:108	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	3	12	theme	better	661:666	arg1	colour					668:673	better colour	661:673	better colour	661:673	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	3	13	theme	xanthan	517:523	arg1	gum					525:527	xanthan gum	517:527	xanthan gum	517:527	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	14	theme	pasta	1180:1184	arg1	yellowness					1186:1195	uncooked pasta yellowness	1171:1195	uncooked pasta yellowness	1171:1195	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	15	theme	gum	1102:1104	arg1	carboxymethylcellulose					1121:1142	carboxymethylcellulose	1121:1142	carboxymethylcellulose (over 0.6% w/w)	1121:1158	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	15	theme	gum	1102:1104	arg1	combination					1079:1089	a combination	1077:1089	(ii) a combination of xanthan gum (0.8% w/w)	1072:1115	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	15	theme	gum	1102:1104	arg1	gum					955:957	xanthan gum	947:957	: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour	941:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	0	16	from	Significance	48:59	arg1	features					101:108	pasta technological features	81:108	pasta technological features	81:108	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	3	17	theme	surface	425:431	arg1	methodology					433:443	randomised response surface methodology	405:443	randomised response surface methodology	405:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	1	18	theme	fresh	161:165	arg1	pasta					167:171	fibre-enriched fresh pasta	146:171	fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties	146:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	4	19	theme	bran	786:789	arg1	composition					752:762	The proximate chemical composition	729:762	The proximate chemical composition of wheat semolina and bran	729:789	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	2	20	theme	Wheat	276:280	arg1	semolina					282:289	Wheat semolina	276:289	Wheat semolina	276:289	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	5	21	dep	gum	955:957	arg1	i					944:944	i	944:944	i	944:944	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	0	22	theme	bran-enriched	11:23	arg1	tagliatelle					35:45	Micronised bran-enriched fresh egg tagliatelle	0:45	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	3	23	theme	locust	533:538	arg1	gum					545:547	locust bean gum	533:547	locust bean gum	533:547	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	3	24	theme	colour	668:673	arg1	characteristics					687:701	better colour and texture characteristics	661:701	better colour and texture characteristics	661:701	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	0	25	theme	Micronised	0:9	arg1	tagliatelle					35:45	Micronised bran-enriched fresh egg tagliatelle	0:45	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	5	26	theme	w/w	969:971	arg1	%					967:967	0.6% w/w	964:971	0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour	964:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	2	27	theme	particle	314:321	arg1	size					323:326	fine particle size	309:326	fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w)	309:376	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	0	28	theme	egg	31:33	arg1	tagliatelle					35:45	Micronised bran-enriched fresh egg tagliatelle	0:45	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	1	29	with	bran	199:202	arg1	properties					264:273	appropriate techno-functional properties	234:273	appropriate techno-functional properties	234:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	3	30	used	used	449:452	arg2	design					393:398	A Box-Behnken design	379:398	A Box-Behnken design with randomised response surface methodology	379:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	2	31	theme	fine	309:312	arg1	size					323:326	fine particle size	309:326	fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w)	309:376	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	1	32	theme	micronised	182:191	arg1	bran					199:202	micronised wheat bran	182:202	micronised wheat bran	182:202	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	0	33	theme	fresh	25:29	arg1	tagliatelle					35:45	Micronised bran-enriched fresh egg tagliatelle	0:45	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	5	34	theme	surface	887:893	arg1	methodology					895:905	the response surface methodology	874:905	the response surface methodology analysis	874:914	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	35	theme	maximum	600:606	arg1	values					608:613	maximum values	600:613	maximum values for water gain and swelling index	600:647	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	1	36	theme	wheat	193:197	arg1	bran					199:202	micronised wheat bran	182:202	micronised wheat bran	182:202	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	5	37	with	pasta	1000:1004	arg1	behaviour					1061:1069	superior cooking behaviour	1044:1069	superior cooking behaviour	1044:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	5	37	with	pasta	1000:1004	arg1	structure					1030:1038	a better developed structure	1011:1038	a better developed structure	1011:1038	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	38	theme	texture	679:685	arg1	characteristics					687:701	better colour and texture characteristics	661:701	better colour and texture characteristics	661:701	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	4	39	theme	wheat	767:771	arg1	semolina					773:780	wheat semolina	767:780	wheat semolina	767:780	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	5	40	theme	uncooked	1171:1178	arg1	yellowness					1186:1195	uncooked pasta yellowness	1171:1195	uncooked pasta yellowness	1171:1195	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	41	theme	bean	540:543	arg1	gum					545:547	locust bean gum	533:547	locust bean gum	533:547	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	3	42	theme	Box-Behnken	381:391	arg1	design					393:398	A Box-Behnken design	379:398	A Box-Behnken design with randomised response surface methodology	379:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	43	theme	superior	1044:1051	arg1	behaviour					1061:1069	superior cooking behaviour	1044:1069	superior cooking behaviour	1044:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	44	theme	pasta	560:564	arg1	attributes					566:575	pasta attributes	560:575	pasta attributes	560:575	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	3	45	theme	randomised	405:414	arg1	methodology					433:443	randomised response surface methodology	405:443	randomised response surface methodology	405:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	46	theme	cooking	1053:1059	arg1	behaviour					1061:1069	superior cooking behaviour	1044:1069	superior cooking behaviour	1044:1069	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	4	47	theme	semolina	773:780	arg1	composition					752:762	The proximate chemical composition	729:762	The proximate chemical composition of wheat semolina and bran	729:789	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	3	48	theme	gum	545:547	arg1	combination					478:488	a suitable combination	467:488	a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking	467:726	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	49	theme	methodology	895:905	arg1	analysis					907:914	the response surface methodology analysis	874:914	the response surface methodology analysis	874:914	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	4	50	theme	pasta	841:845	arg1	microstructure					814:827	the microstructure	810:827	the microstructure of uncooked pasta	810:845	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	4	50	theme	pasta	841:845	arg1	well					863:866	well	863:866	well	863:866	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	0	51	dep	tagliatelle	35:45	arg1	Significance					48:59	Significance	48:59	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	5	52	theme	response	878:885	arg1	methodology					895:905	the response surface methodology	874:905	the response surface methodology analysis	874:914	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	4	53	theme	proximate	733:741	arg1	composition					752:762	The proximate chemical composition	729:762	The proximate chemical composition of wheat semolina and bran	729:789	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	3	54	theme	minimum	578:584	arg1	loss					594:597	minimum cooking loss	578:597	minimum cooking loss	578:597	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	2	55	dep	size	323:326	arg1	%					331:331	50%	329:331	50%	329:331	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	2	55	dep	size	323:326	arg1	w/w					373:375	w/w	373:375	w/w	373:375	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	2	55	dep	size	323:326	arg1	%					370:370	up to 11.54%	359:370	up to 11.54% (w/w)	359:376	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	2	55	dep	size	323:326	arg1	bran					352:355	wheat bran	346:355	wheat bran	346:355	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	3	56	theme	gum	525:527	arg1	combination					478:488	a suitable combination	467:488	a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking	467:726	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	1	57	theme	durum	208:212	arg1	semolina					220:227	durum wheat semolina	208:227	durum wheat semolina	208:227	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	3	58	theme	carboxymethylcellulose	493:514	arg1	combination					478:488	a suitable combination	467:488	a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking	467:726	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	59	dep	combination	1079:1089	arg1	ii					1073:1074	ii	1073:1074	ii	1073:1074	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	3	60	theme	cooking	586:592	arg1	loss					594:597	minimum cooking loss	578:597	minimum cooking loss	578:597	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	1	61	with	semolina	220:227	arg1	properties					264:273	appropriate techno-functional properties	234:273	appropriate techno-functional properties	234:273	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	1	62	theme	work	126:129	arg1	aim					115:117	The aim	111:117	The aim of the work	111:129	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	5	63	theme	%	1153:1153	arg1	 w/w					1154:1157	0.6% w/w	1150:1157	0.6% w/w	1150:1157	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	1	64	theme	wheat	214:218	arg1	semolina					220:227	durum wheat semolina	208:227	durum wheat semolina	208:227	The aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties.					
29307227	0	65	theme	addition	69:76	arg1	Significance					48:59	Significance	48:59	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.	0:109	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	3	66	theme	water	619:623	arg1	gain					625:628	water gain	619:628	water gain	619:628	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	2	67	dep	11.54	365:369	arg1	to					362:363	to	362:363	to	362:363	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
29307227	3	68	theme	response	416:423	arg1	methodology					433:443	randomised response surface methodology	405:443	randomised response surface methodology	405:443	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	3	69	theme	suitable	469:476	arg1	combination					478:488	a suitable combination	467:488	a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking	467:726	A Box-Behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum values for water gain and swelling index, as well as better colour and texture characteristics before and after cooking.					
29307227	5	70	theme	0.6	1150:1152	arg1	%					1153:1153	%	1153:1153	%	1153:1153	From the response surface methodology analysis, it is recommended to use: (i) xanthan gum over 0.6% w/w as it led to bran-enriched pasta with a better developed structure and superior cooking behaviour, (ii) a combination of xanthan gum (0.8% w/w) and carboxymethylcellulose (over 0.6% w/w) to enhance uncooked pasta yellowness.					
29307227	0	71	theme	gums	64:67	arg1	addition					69:76	gums addition	64:76	gums addition	64:76	Micronised bran-enriched fresh egg tagliatelle: Significance of gums addition on pasta technological features.					
29307227	4	72	theme	chemical	743:750	arg1	composition					752:762	The proximate chemical composition	729:762	The proximate chemical composition of wheat semolina and bran	729:789	The proximate chemical composition of wheat semolina and bran was determined and the microstructure of uncooked pasta was observed as well.					
29307227	2	73	theme	wheat	346:350	arg1	bran					352:355	wheat bran	346:355	wheat bran	346:355	Wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w).					
30529352	7	0	theme	L929	1270:1273	arg1	cells					1275:1279	L929 cells	1270:1279	L929 cells	1270:1279	Moreover, there was no statistically significant difference between the material treatment and the blank control group, indicating that the material almost showed no toxicity to L929 cells.					
30529352	4	1	theme	FSHPs‑silver	591:602	arg1	nanoparticles					604:616	The FSHPs‑silver nanoparticles	587:616	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution	587:660	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	4	1	theme	FSHPs‑silver	591:602	arg1	FSHPs-Ag					619:626	FSHPs-Ag	619:626	FSHPs-Ag	619:626	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	0	2	theme	defects	193:199	arg1	regeneration					148:159	the regeneration	144:159	the regeneration of infected full-thickness skin defects	144:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	0	3	with	composites	94:103	arg1	chitosan					110:117	chitosan	110:117	chitosan	110:117	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	3	4	theme	silver	534:539	arg1	AgNPs					556:560	AgNPs	556:560	AgNPs	556:560	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	4	theme	silver	534:539	arg1	nanoparticles					541:553	silver nanoparticles	534:553	silver nanoparticles (AgNPs)	534:561	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	1	5	theme	antibacterial	265:277	arg1	dressings					279:287	antibacterial dressings	265:287	antibacterial dressings	265:287	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	7	6	theme	control	1197:1203	arg1	group					1205:1209	the blank control group	1187:1209	the blank control group	1187:1209	Moreover, there was no statistically significant difference between the material treatment and the blank control group, indicating that the material almost showed no toxicity to L929 cells.					
30529352	9	7	theme	wound	1556:1560	arg1	material					1571:1578	an ideal wound dressing material	1547:1578	an ideal wound dressing material	1547:1578	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	3	8	used	used	492:495	arg2	polysaccharides					463:477	sanghuang polysaccharides	453:477	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	8	used	used	492:495	arg2	FSHPs					480:484	FSHPs	480:484	FSHPs	480:484	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	8	used	used	492:495	arg2	agent					517:521	a green reducing agent	500:521	a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm	500:584	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	0	9	with	sanghuang	80:88	arg1	chitosan					110:117	chitosan	110:117	chitosan	110:117	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	9	10	theme	dressing	1562:1569	arg1	material					1571:1578	an ideal wound dressing material	1547:1578	an ideal wound dressing material	1547:1578	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	9	11	theme	wound	1391:1395	arg1	contraction					1397:1407	wound contraction	1391:1407	wound contraction	1391:1407	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	8	12	theme	dressing	1318:1325	arg1	wounds					1334:1339	dressing animal wounds	1318:1339	dressing animal wounds	1318:1339	Finally, this material was used for dressing animal wounds.					
30529352	5	13	theme	internal	760:767	arg1	pores					769:773	The internal pores	756:773	The internal pores of CS-FSHPs-Ag	756:788	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	5	14	theme	retention	849:857	arg1	properties					859:868	good swelling and water retention properties	825:868	good swelling and water retention properties	825:868	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	7	15	theme	significant	1129:1139	arg1	difference					1141:1150	no statistically significant difference	1112:1150	no statistically significant difference between the material treatment and the blank control group	1112:1209	Moreover, there was no statistically significant difference between the material treatment and the blank control group, indicating that the material almost showed no toxicity to L929 cells.					
30529352	9	16	theme	tissue	1422:1427	arg1	growth					1429:1434	internal tissue growth	1413:1434	internal tissue growth	1413:1434	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	9	17	theme	Aquacel®	1472:1479	arg1	Ag					1481:1482	Aquacel® Ag	1472:1482	Aquacel® Ag	1472:1482	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	6	18	theme	appropriate	962:972	arg1	concentration					974:986	the appropriate concentration	958:986	the appropriate concentration of AgNPs required for CS-FSHPs-Ag to inhibit Escherichia coli and Staphylococcus aureus	958:1074	Based on the experimental results, the appropriate concentration of AgNPs required for CS-FSHPs-Ag to inhibit Escherichia coli and Staphylococcus aureus was determined.					
30529352	2	19	theme	traditional	339:349	arg1	methods					351:357	traditional methods	339:357	traditional methods for reducing silver nanoparticles	339:391	However, traditional methods for reducing silver nanoparticles are usually toxic.					
30529352	5	20	theme	good	825:828	arg1	properties					859:868	good swelling and water retention properties	825:868	good swelling and water retention properties	825:868	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	6	21	theme	AgNPs	991:995	arg1	concentration					974:986	the appropriate concentration	958:986	the appropriate concentration of AgNPs required for CS-FSHPs-Ag to inhibit Escherichia coli and Staphylococcus aureus	958:1074	Based on the experimental results, the appropriate concentration of AgNPs required for CS-FSHPs-Ag to inhibit Escherichia coli and Staphylococcus aureus was determined.					
30529352	1	22	theme	recent	205:210	arg1	years					212:216	recent years	205:216	recent years	205:216	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	5	23	theme	swelling	830:837	arg1	properties					859:868	good swelling and water retention properties	825:868	good swelling and water retention properties	825:868	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	1	24	theme	antibiotic	298:307	arg1	problems					320:327	antibiotic resistance problems	298:327	antibiotic resistance problems	298:327	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	5	25	theme	water	843:847	arg1	retention					849:857	water retention	843:857	water retention	843:857	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	1	26	theme	resistance	309:318	arg1	problems					320:327	antibiotic resistance problems	298:327	antibiotic resistance problems	298:327	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	0	27	from	composites	94:103	arg1	polysaccharides					44:58	polysaccharides	44:58	polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects	44:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	9	28	theme	ideal	1550:1554	arg1	material					1571:1578	an ideal wound dressing material	1547:1578	an ideal wound dressing material	1547:1578	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	4	29	theme	chitosan	644:651	arg1	solution					653:660	chitosan solution	644:660	chitosan solution	644:660	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	4	30	theme	chitosan-FSHPs-Ag	723:739	arg1	dressing					711:718	a porous sponge dressing	695:718	a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag)	695:753	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	9	31	contain	has	1522:1524	arg1	CS-FSHPs-Ag					1510:1520	the CS-FSHPs-Ag	1506:1520	the CS-FSHPs-Ag	1506:1520	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	9	31	contain	has	1522:1524	arg2	potential					1534:1542	a great potential	1526:1542	a great potential as an ideal wound dressing material	1526:1578	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	3	32	dep	polysaccharides	463:477	arg1	Sanghuangporus					438:451	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	1	33	theme	silver	219:224	arg1	nanoparticles					226:238	silver nanoparticles	219:238	silver nanoparticles	219:238	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	3	34	theme	sanghuang	453:461	arg1	agent					517:521	a green reducing agent	500:521	a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm	500:584	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	34	theme	sanghuang	453:461	arg1	FSHPs					480:484	FSHPs	480:484	FSHPs	480:484	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	34	theme	sanghuang	453:461	arg1	polysaccharides					463:477	sanghuang polysaccharides	453:477	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	7	35	theme	blank	1191:1195	arg1	group					1205:1209	the blank control group	1187:1209	the blank control group	1187:1209	Moreover, there was no statistically significant difference between the material treatment and the blank control group, indicating that the material almost showed no toxicity to L929 cells.					
30529352	0	36	from	sanghuang	80:88	arg1	polysaccharides					44:58	polysaccharides	44:58	polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects	44:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	4	37	with	composite	629:637	arg1	solution					653:660	chitosan solution	644:660	chitosan solution	644:660	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	6	38	theme	experimental	936:947	arg1	results					949:955	the experimental results	932:955	the experimental results	932:955	Based on the experimental results, the appropriate concentration of AgNPs required for CS-FSHPs-Ag to inhibit Escherichia coli and Staphylococcus aureus was determined.					
30529352	7	39	theme	material	1164:1171	arg1	treatment					1173:1181	the material treatment	1160:1181	the material treatment	1160:1181	Moreover, there was no statistically significant difference between the material treatment and the blank control group, indicating that the material almost showed no toxicity to L929 cells.					
30529352	5	40	theme	moist	893:897	arg1	environment					899:909	a moist environment	891:909	a moist environment for wounds	891:920	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	0	41	theme	infected	164:171	arg1	defects					193:199	infected full-thickness skin defects	164:199	infected full-thickness skin defects	164:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	5	42	contain	had	821:823	arg1	pores					769:773	The internal pores	756:773	The internal pores of CS-FSHPs-Ag	756:788	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	5	42	contain	had	821:823	arg2	properties					859:868	good swelling and water retention properties	825:868	good swelling and water retention properties	825:868	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	8	43	used	used	1309:1312	arg2	material					1296:1303	this material	1291:1303	this material	1291:1303	Finally, this material was used for dressing animal wounds.					
30529352	2	44	theme	silver	372:377	arg1	nanoparticles					379:391	reducing silver nanoparticles	363:391	reducing silver nanoparticles	363:391	However, traditional methods for reducing silver nanoparticles are usually toxic.					
30529352	9	45	theme	great	1528:1532	arg1	potential					1534:1542	a great potential	1526:1542	a great potential as an ideal wound dressing material	1526:1578	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	1	46	used	used	257:260	arg2	nanoparticles					226:238	silver nanoparticles	219:238	silver nanoparticles	219:238	In recent years, silver nanoparticles have widely been used in antibacterial dressings to solve antibiotic resistance problems.					
30529352	2	47	theme	reducing	363:370	arg1	nanoparticles					379:391	reducing silver nanoparticles	363:391	reducing silver nanoparticles	363:391	However, traditional methods for reducing silver nanoparticles are usually toxic.					
30529352	3	48	theme	3-35 nm	578:584	arg1	size					570:573	a size	568:573	a size of 3-35 nm	568:584	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	0	49	theme	skin	188:191	arg1	defects					193:199	infected full-thickness skin defects	164:199	infected full-thickness skin defects	164:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	5	50	theme	CS-FSHPs-Ag	778:788	arg1	pores					769:773	The internal pores	756:773	The internal pores of CS-FSHPs-Ag	756:788	The internal pores of CS-FSHPs-Ag were between 50 and 100 μm and had good swelling and water retention properties, which could provide a moist environment for wounds.					
30529352	3	51	theme	green	502:506	arg1	agent					517:521	a green reducing agent	500:521	a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm	500:584	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	51	theme	green	502:506	arg1	polysaccharides					463:477	sanghuang polysaccharides	453:477	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	9	52	theme	internal	1413:1420	arg1	growth					1429:1434	internal tissue growth	1413:1434	internal tissue growth	1413:1434	The results showed that the CS-FSHPs-Ag promoted wound contraction and internal tissue growth better than the wounds treated with Aquacel® Ag, which indicated that the CS-FSHPs-Ag has a great potential as an ideal wound dressing material.					
30529352	0	53	theme	full-thickness	173:186	arg1	defects					193:199	infected full-thickness skin defects	164:199	infected full-thickness skin defects	164:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	3	54	theme	reducing	508:515	arg1	agent					517:521	a green reducing agent	500:521	a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm	500:584	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	3	54	theme	reducing	508:515	arg1	polysaccharides					463:477	sanghuang polysaccharides	453:477	Sanghuangporus sanghuang polysaccharides (FSHPs)	438:485	To overcome this problem, Sanghuangporus sanghuang polysaccharides (FSHPs) were used as a green reducing agent to prepare silver nanoparticles (AgNPs) with a size of 3-35 nm.					
30529352	8	55	theme	animal	1327:1332	arg1	wounds					1334:1339	dressing animal wounds	1318:1339	dressing animal wounds	1318:1339	Finally, this material was used for dressing animal wounds.					
30529352	0	56	dep	nanoparticles	7:19	arg1	synthesized					29:39	synthesized	29:39	nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects	7:199	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	4	57	theme	sponge	704:709	arg1	dressing					711:718	a porous sponge dressing	695:718	a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag)	695:753	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	0	58	theme	Sanghuangporus	65:78	arg1	sanghuang					80:88	Sanghuangporus sanghuang	65:88	Sanghuangporus sanghuang	65:88	Silver nanoparticles in situ synthesized by polysaccharides from Sanghuangporus sanghuang and composites with chitosan to prepare scaffolds for the regeneration of infected full-thickness skin defects.					
30529352	4	59	theme	porous	697:702	arg1	dressing					711:718	a porous sponge dressing	695:718	a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag)	695:753	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	4	60	theme	composite	629:637	arg1	nanoparticles					604:616	The FSHPs‑silver nanoparticles	587:616	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution	587:660	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
30529352	4	60	theme	composite	629:637	arg1	FSHPs-Ag					619:626	FSHPs-Ag	619:626	FSHPs-Ag	619:626	The FSHPs‑silver nanoparticles (FSHPs-Ag) composite with chitosan solution were then freeze-dried to obtain a porous sponge dressing of chitosan-FSHPs-Ag (CS-FSHPs-Ag).					
29701014	0	0	theme	alternate	80:88	arg1	soaking					90:96	alternate soaking	80:96	alternate soaking in solutions of calcium/magnesium and carbonate ion solutions	80:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	2	1	theme	phosphate	341:349	arg1	biomaterials					351:362	calcium phosphate biomaterials	333:362	calcium phosphate biomaterials	333:362	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	7	2	theme	Ca	984:985	arg1	ratio					1002:1006	Ca : Mg elemental ratio	984:1006	Ca : Mg elemental ratio in the mineral formed	984:1028	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	9	3	dep	formed	1238:1243	arg1	decreased					1291:1299	decreased	1291:1299	decreased the total amount of the mineral formed and its crystallinity	1291:1360	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	3	dep	formed	1238:1243	arg1	led					1245:1247	led	1245:1247	led to the formation of magnesian calcite	1245:1285	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	2	4	theme	calcium	333:339	arg1	biomaterials					351:362	calcium phosphate biomaterials	333:362	calcium phosphate biomaterials	333:362	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	10	5	theme	MC3T3-E1	1474:1481	arg1	cells					1499:1503	MC3T3-E1 osteoblast-like cells	1474:1503	MC3T3-E1 osteoblast-like cells	1474:1503	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	7	6	from	ratio	1002:1006	arg1	mineral					1015:1021	mineral	1015:1021	mineral	1015:1021	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	3	7	contain	containing	515:524	arg1	carbonates					504:513	carbonates	504:513	carbonates containing different amounts of calcium (Ca) and magnesium (Mg)	504:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	7	contain	containing	515:524	arg2	amounts					536:542	different amounts	526:542	different amounts of calcium (Ca) and magnesium (Mg)	526:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	7	contain	containing	515:524	arg2	Mg					575:576	Mg	575:576	Mg	575:576	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	7	contain	containing	515:524	arg2	calcium					547:553	calcium	547:553	calcium (Ca)	547:558	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	7	contain	containing	515:524	arg2	Ca					556:557	Ca	556:557	Ca	556:557	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	7	contain	containing	515:524	arg2	magnesium					564:572	magnesium	564:572	magnesium (Mg)	564:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	8	theme	calcium	547:553	arg1	calcium					547:553	calcium	547:553	calcium (Ca)	547:558	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	8	theme	calcium	547:553	arg1	amounts					536:542	different amounts	526:542	different amounts of calcium (Ca) and magnesium (Mg)	526:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	8	theme	calcium	547:553	arg1	Mg					575:576	Mg	575:576	Mg	575:576	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	8	theme	calcium	547:553	arg1	Ca					556:557	Ca	556:557	Ca	556:557	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	8	theme	calcium	547:553	arg1	magnesium					564:572	magnesium	564:572	magnesium (Mg)	564:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	4	9	theme	alternate	699:707	arg1	cycle					717:721	This alternate soaking cycle	694:721	This alternate soaking cycle	694:721	This alternate soaking cycle was repeated five times.					
29701014	6	10	theme	sample	928:933	arg1	groups					935:940	all sample groups	924:940	all sample groups subjected to the alternate soaking cycle	924:981	Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle.					
29701014	10	11	theme	cells	1499:1503	arg1	proliferation					1457:1469	proliferation	1457:1469	proliferation of MC3T3-E1 osteoblast-like cells	1457:1503	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	10	11	theme	cells	1499:1503	arg1	differentiation					1508:1522	differentiation	1508:1522	differentiation after 7 days	1508:1535	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	3	12	theme	ion	680:682	arg1	hydrogels					472:480	gellan gum hydrogels	461:480	gellan gum hydrogels	461:480	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	12	theme	ion	680:682	arg1	solution					684:691	a carbonate ion solution	668:691	a carbonate ion solution	668:691	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	5	13	theme	molar	824:828	arg1	ratios					830:835	different molar ratios	814:835	different molar ratios of Ca	814:841	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	10	14	theme	osteoblast-like	1483:1497	arg1	cells					1499:1503	MC3T3-E1 osteoblast-like cells	1474:1503	MC3T3-E1 osteoblast-like cells	1474:1503	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	9	15	from	content	1215:1221	arg1	mineral					1230:1236	mineral	1230:1236	mineral	1230:1236	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	16	theme	mineral	1325:1331	arg1	crystallinity					1348:1360	its crystallinity	1344:1360	its crystallinity	1344:1360	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	16	theme	mineral	1325:1331	arg1	amount					1311:1316	the total amount	1301:1316	the total amount of the mineral formed	1301:1338	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	16	theme	mineral	1325:1331	arg1	mineral					1325:1331	mineral	1325:1331	mineral	1325:1331	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	8	17	theme	Mg	1120:1121	arg1	absence					1109:1115	the absence	1105:1115	the absence of Mg	1105:1121	Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite.					
29701014	1	18	theme	hydrogels	179:187	arg1	Mineralization					161:174	Mineralization	161:174	Mineralization of hydrogels	161:187	Mineralization of hydrogels is desirable prior to applications in bone regeneration.					
29701014	5	19	theme	different	753:761	arg1	solutions					792:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	8	20	from	CaCO3	1141:1145	arg1	form					1154:1157	the form	1150:1157	the form of a mixture of calcite and vaterite	1150:1194	Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite.					
29701014	10	21	theme	Mg	1402:1403	arg1	content					1405:1411	Hydrogel mineralization and increasing Mg content	1363:1411	content	1405:1411	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	10	22	theme	increasing	1391:1400	arg1	content					1405:1411	Hydrogel mineralization and increasing Mg content	1363:1411	content	1405:1411	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	2	23	theme	regeneration	274:285	arg1	CaCO3					246:250	CaCO3	246:250	CaCO3	246:250	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	2	23	theme	regeneration	274:285	arg1	material					287:294	a widely used bone regeneration material	255:294	a widely used bone regeneration material	255:294	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	2	24	theme	bone-forming	378:389	arg1	adhesion					396:403	bone-forming cell adhesion	378:403	bone-forming cell adhesion	378:403	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	5	25	theme	calcium	763:769	arg1	solutions					792:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	3	26	theme	carbonate	670:678	arg1	hydrogels					472:480	gellan gum hydrogels	461:480	gellan gum hydrogels	461:480	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	26	theme	carbonate	670:678	arg1	solution					684:691	a carbonate ion solution	668:691	a carbonate ion solution	668:691	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	10	27	theme	Hydrogel	1363:1370	arg1	mineralization					1372:1385	Hydrogel mineralization and increasing Mg content	1363:1411	mineralization	1372:1385	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	5	28	contain	containing	803:812	arg1	solutions					792:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	5	28	contain	containing	803:812	arg2	ratios					830:835	different molar ratios	814:835	different molar ratios of Ca	814:841	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	5	29	theme	free	876:879	arg1	Ca					873:874	Ca free	873:879	Ca free	873:879	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	8	30	theme	mixture	1164:1170	arg1	form					1154:1157	the form	1150:1157	the form of a mixture of calcite and vaterite	1150:1194	Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite.					
29701014	6	31	theme	alternate	959:967	arg1	cycle					977:981	the alternate soaking cycle	955:981	the alternate soaking cycle	955:981	Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle.					
29701014	3	32	theme	magnesium	630:638	arg1	solution					644:651	a calcium and/or magnesium ion solution	613:651	solution	644:651	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	33	theme	magnesium	564:572	arg1	calcium					547:553	calcium	547:553	calcium (Ca)	547:558	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	33	theme	magnesium	564:572	arg1	amounts					536:542	different amounts	526:542	different amounts of calcium (Ca) and magnesium (Mg)	526:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	33	theme	magnesium	564:572	arg1	Mg					575:576	Mg	575:576	Mg	575:576	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	33	theme	magnesium	564:572	arg1	Ca					556:557	Ca	556:557	Ca	556:557	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	33	theme	magnesium	564:572	arg1	magnesium					564:572	magnesium	564:572	magnesium (Mg)	564:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	1	34	theme	prior	202:206	arg1	desirable					192:200	desirable	192:200	desirable prior to applications in bone regeneration	192:243	Mineralization of hydrogels is desirable prior to applications in bone regeneration.					
29701014	0	35	theme	gum	25:27	arg1	Mineralization					0:13	Mineralization	0:13	Mineralization of gellan gum	0:27	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	3	36	from	mineralized	487:497	arg1	study					454:458	this study	449:458	this study	449:458	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	4	37	theme	soaking	709:715	arg1	cycle					717:721	This alternate soaking cycle	694:721	This alternate soaking cycle	694:721	This alternate soaking cycle was repeated five times.					
29701014	8	38	theme	calcite	1175:1181	arg1	mixture					1164:1170	a mixture	1162:1170	a mixture of calcite and vaterite	1162:1194	Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite.					
29701014	2	39	theme	bone	427:430	arg1	regeneration					432:443	bone regeneration	427:443	bone regeneration	427:443	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	6	40	theme	mineral	906:912	arg1	Carbonate					896:904	Carbonate mineral	896:912	Carbonate mineral	896:912	Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle.					
29701014	0	41	theme	calcium/magnesium	114:130	arg1	solutions					101:109	solutions	101:109	solutions of calcium/magnesium and carbonate ion solutions	101:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	0	42	dep	calcium/magnesium	114:130	arg1	solutions					150:158	ion solutions	146:158	ion solutions	146:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	10	43	from	content	1405:1411	arg1	mineral					1416:1422	mineral	1416:1422	mineral	1416:1422	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	3	44	theme	gellan	461:466	arg1	calcium					615:621	a calcium and/or magnesium ion solution	613:651	calcium	615:621	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	44	theme	gellan	461:466	arg1	hydrogels					472:480	gellan gum hydrogels	461:480	gellan gum hydrogels	461:480	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	44	theme	gellan	461:466	arg1	solution					684:691	a carbonate ion solution	668:691	a carbonate ion solution	668:691	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	0	45	theme	ion	146:148	arg1	solutions					150:158	ion solutions	146:158	ion solutions	146:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	8	46	theme	vaterite	1187:1194	arg1	mixture					1164:1170	a mixture	1162:1170	a mixture of calcite and vaterite	1162:1194	Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite.					
29701014	5	47	theme	Ca	840:841	arg1	ratios					830:835	different molar ratios	814:835	different molar ratios of Ca	814:841	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	7	48	theme	respective	1053:1062	arg1	solution					1077:1084	the respective mineralizing solution	1049:1084	the respective mineralizing solution	1049:1084	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	2	49	theme	cell	391:394	arg1	adhesion					396:403	bone-forming cell adhesion	378:403	bone-forming cell adhesion	378:403	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	0	50	theme	carbonate	136:144	arg1	solutions					101:109	solutions	101:109	solutions of calcium/magnesium and carbonate ion solutions	101:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	5	51	theme	magnesium	778:786	arg1	ion					788:790	magnesium ion	778:790	magnesium ion	778:790	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	5	52	theme	different	814:822	arg1	ratios					830:835	different molar ratios	814:835	different molar ratios of Ca	814:841	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	1	53	theme	bone	227:230	arg1	regeneration					232:243	bone regeneration	227:243	bone regeneration	227:243	Mineralization of hydrogels is desirable prior to applications in bone regeneration.					
29701014	7	54	theme	Mg	989:990	arg1	ratio					1002:1006	Ca : Mg elemental ratio	984:1006	Ca : Mg elemental ratio in the mineral formed	984:1028	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	10	55	from	mineralization	1372:1385	arg1	mineral					1416:1422	mineral	1416:1422	mineral	1416:1422	Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.					
29701014	5	56	theme	ion	788:790	arg1	solutions					792:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions	748:800	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	3	57	from	study	454:458	arg1	mineralized					487:497	mineralized	487:497	mineralized	487:497	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	7	58	theme	elemental	992:1000	arg1	ratio					1002:1006	Ca : Mg elemental ratio	984:1006	Ca : Mg elemental ratio in the mineral formed	984:1028	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	0	59	theme	calcium	44:50	arg1	carbonates					66:75	calcium and magnesium carbonates	44:75	calcium and magnesium carbonates	44:75	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	9	60	theme	magnesian	1269:1277	arg1	calcite					1279:1285	magnesian calcite	1269:1285	magnesian calcite	1269:1285	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	61	theme	Mg	1212:1213	arg1	content					1215:1221	the Mg content	1208:1221	the Mg content in the mineral	1208:1236	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	3	62	theme	ion	640:642	arg1	solution					644:651	a calcium and/or magnesium ion solution	613:651	solution	644:651	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	5	63	theme	free	865:868	arg1	Mg					862:863	Mg	862:863	Mg free to Ca free	862:879	Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared.					
29701014	9	64	theme	calcite	1279:1285	arg1	formation					1256:1264	the formation	1252:1264	the formation of magnesian calcite	1252:1285	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	7	65	theme	mineralizing	1064:1075	arg1	solution					1077:1084	the respective mineralizing solution	1049:1084	the respective mineralizing solution	1049:1084	Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution.					
29701014	2	66	theme	bone	269:272	arg1	CaCO3					246:250	CaCO3	246:250	CaCO3	246:250	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	2	66	theme	bone	269:272	arg1	material					287:294	a widely used bone regeneration material	255:294	a widely used bone regeneration material	255:294	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	1	67	from	applications	211:222	arg1	regeneration					232:243	bone regeneration	227:243	bone regeneration	227:243	Mineralization of hydrogels is desirable prior to applications in bone regeneration.					
29701014	3	68	theme	gum	468:470	arg1	calcium					615:621	a calcium and/or magnesium ion solution	613:651	calcium	615:621	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	68	theme	gum	468:470	arg1	hydrogels					472:480	gellan gum hydrogels	461:480	gellan gum hydrogels	461:480	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	68	theme	gum	468:470	arg1	solution					684:691	a carbonate ion solution	668:691	a carbonate ion solution	668:691	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	6	69	theme	soaking	969:975	arg1	cycle					977:981	the alternate soaking cycle	955:981	the alternate soaking cycle	955:981	Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle.					
29701014	3	70	dep	mineralized	487:497	arg1	solution					684:691	a carbonate ion solution	668:691	a carbonate ion solution	668:691	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	70	dep	mineralized	487:497	arg1	calcium					615:621	a calcium and/or magnesium ion solution	613:651	calcium	615:621	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	70	dep	mineralized	487:497	arg1	hydrogels					472:480	gellan gum hydrogels	461:480	gellan gum hydrogels	461:480	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	70	dep	mineralized	487:497	arg1	solution					644:651	a calcium and/or magnesium ion solution	613:651	solution	644:651	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	2	71	theme	used	264:267	arg1	CaCO3					246:250	CaCO3	246:250	CaCO3	246:250	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	2	71	theme	used	264:267	arg1	material					287:294	a widely used bone regeneration material	255:294	a widely used bone regeneration material	255:294	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	3	72	theme	different	526:534	arg1	calcium					547:553	calcium	547:553	calcium (Ca)	547:558	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	72	theme	different	526:534	arg1	amounts					536:542	different amounts	526:542	different amounts of calcium (Ca) and magnesium (Mg)	526:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	72	theme	different	526:534	arg1	Mg					575:576	Mg	575:576	Mg	575:576	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	72	theme	different	526:534	arg1	Ca					556:557	Ca	556:557	Ca	556:557	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	72	theme	different	526:534	arg1	magnesium					564:572	magnesium	564:572	magnesium (Mg)	564:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	3	73	with	mineralized	487:497	arg1	carbonates					504:513	carbonates	504:513	carbonates containing different amounts of calcium (Ca) and magnesium (Mg)	504:577	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	0	74	from	soaking	90:96	arg1	solutions					101:109	solutions	101:109	solutions of calcium/magnesium and carbonate ion solutions	101:158	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	3	75	theme	alternate	582:590	arg1	soaking					592:598	alternate soaking	582:598	alternate soaking in	582:601	In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution.					
29701014	0	76	theme	magnesium	56:64	arg1	carbonates					66:75	calcium and magnesium carbonates	44:75	calcium and magnesium carbonates	44:75	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	0	77	theme	gellan	18:23	arg1	gum					25:27	gellan gum	18:27	gellan gum	18:27	Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.					
29701014	2	78	theme	biomaterials	351:362	arg1	component					320:328	a component	318:328	a component of calcium phosphate biomaterials	318:362	CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.					
29701014	9	79	theme	total	1305:1309	arg1	amount					1311:1316	the total amount	1301:1316	the total amount of the mineral formed	1301:1338	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29701014	9	79	theme	total	1305:1309	arg1	mineral					1325:1331	mineral	1325:1331	mineral	1325:1331	Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity.					
29366898	5	0	theme	-α-d-Galp-	709:718	arg1	1→					740:741	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	1→	740:741	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	0	1	from	activity	39:46	arg1	the					75:77	the	75:77	the	75:77	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	6	2	theme	antioxidant	937:947	arg1	FRAP					956:959	FRAP	956:959	FRAP	956:959	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	6	2	theme	antioxidant	937:947	arg1	power					949:953	ferric-reducing antioxidant power	921:953	ferric-reducing antioxidant power (FRAP)	921:960	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	0	3	from	elucidation	11:21	arg1	the					75:77	the	75:77	the	75:77	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	6	4	theme	ferric-reducing	921:935	arg1	FRAP					956:959	FRAP	956:959	FRAP	956:959	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	6	4	theme	ferric-reducing	921:935	arg1	power					949:953	ferric-reducing antioxidant power	921:953	ferric-reducing antioxidant power (FRAP)	921:960	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	5	5	theme	-β-d-Galp-	691:700	arg1	1→					740:741	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	1→	740:741	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	4	6	theme	-β-d-Galp-	597:606	arg1	backbone					582:589	a backbone	580:589	a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position	580:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	1	7	theme	Sepharose	246:254	arg1	chromatography					274:287	Sepharose 6B gel filtration chromatography	246:287	Sepharose 6B gel filtration chromatography	246:287	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	0	8	theme	Moringa	89:95	arg1	oleifera					97:104	Moringa oleifera	89:104	Moringa oleifera	89:104	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	4	9	from	position	648:655	arg1	chains					634:639	highly branched chains	618:639	highly branched chains at O-4 position	618:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	5	10	theme	4→	702:703	arg1	1→					740:741	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	1→	740:741	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	6	11	theme	scavenging	802:811	arg1	capacity					813:820	DPPH radical scavenging capacity	789:820	DPPH radical scavenging capacity	789:820	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	2	12	theme	MOP-1	390:394	arg1	7.65 × 107 Da					400:412	7.65 × 107 Da	400:412	7.65 × 107 Da	400:412	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	12	theme	MOP-1	390:394	arg1	weight					380:385	the molecular weight	366:385	the molecular weight of MOP-1	366:394	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	6	13	theme	radical	794:800	arg1	capacity					813:820	DPPH radical scavenging capacity	789:820	DPPH radical scavenging capacity	789:820	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	3	14	theme	1:7.32:12.12	521:532	arg1	ratio					512:516	a molar ratio	504:516	a molar ratio of 1:7.32:12.12	504:532	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	5	15	theme	1→	730:731	arg1	1→					740:741	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	1→	740:741	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	1	16	attach	isolated	143:150	arg1	leaves					157:162	leaves	157:162	leaves	157:162	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	1	16	attach	isolated	143:150	arg2	MOP-1					132:136	MOP-1	132:136	MOP-1	132:136	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	1	16	attach	isolated	143:150	arg2	arabinogalactan					115:129	A novel arabinogalactan	107:129	A novel arabinogalactan (MOP-1)	107:137	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	2	17	theme	permeation	311:320	arg1	HPGPC					338:342	HPGPC	338:342	HPGPC	338:342	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	17	theme	permeation	311:320	arg1	chromatography					322:335	High performance gel permeation chromatography	290:335	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	3	18	theme	composition	430:440	arg1	analysis					442:449	Monosaccharide composition analysis	415:449	Monosaccharide composition analysis	415:449	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	0	19	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	1	20	theme	gel	259:261	arg1	chromatography					274:287	Sepharose 6B gel filtration chromatography	246:287	Sepharose 6B gel filtration chromatography	246:287	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	2	21	theme	gel	307:309	arg1	HPGPC					338:342	HPGPC	338:342	HPGPC	338:342	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	21	theme	gel	307:309	arg1	chromatography					322:335	High performance gel permeation chromatography	290:335	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	22	theme	molecular	370:378	arg1	7.65 × 107 Da					400:412	7.65 × 107 Da	400:412	7.65 × 107 Da	400:412	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	22	theme	molecular	370:378	arg1	weight					380:385	the molecular weight	366:385	the molecular weight of MOP-1	366:394	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	0	23	theme	antioxidant	27:37	arg1	activity					39:46	antioxidant activity	27:46	antioxidant activity	27:46	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	1	24	theme	filtration	263:272	arg1	chromatography					274:287	Sepharose 6B gel filtration chromatography	246:287	Sepharose 6B gel filtration chromatography	246:287	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	7	25	theme	significant	1001:1011	arg1	activities					1025:1034	significant antioxidant activities	1001:1034	significant antioxidant activities	1001:1034	The results indicated MOP-1 exhibited significant antioxidant activities.					
29366898	2	26	theme	performance	295:305	arg1	HPGPC					338:342	HPGPC	338:342	HPGPC	338:342	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	26	theme	performance	295:305	arg1	chromatography					322:335	High performance gel permeation chromatography	290:335	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	6	27	theme	cation	889:894	arg1	capacity					907:914	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	7	28	theme	antioxidant	1013:1023	arg1	activities					1025:1034	significant antioxidant activities	1001:1034	significant antioxidant activities	1001:1034	The results indicated MOP-1 exhibited significant antioxidant activities.					
29366898	6	29	theme	antioxidant	748:758	arg1	activities					760:769	Its antioxidant activities	744:769	Its antioxidant activities	744:769	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	2	30	theme	High	290:293	arg1	HPGPC					338:342	HPGPC	338:342	HPGPC	338:342	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	2	30	theme	High	290:293	arg1	chromatography					322:335	High performance gel permeation chromatography	290:335	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	5	31	theme	2→	720:721	arg1	1→					740:741	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	1→	740:741	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	6	32	theme	radical	881:887	arg1	cation					889:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation	823:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	4	33	theme	branched	625:632	arg1	chains					634:639	highly branched chains	618:639	highly branched chains at O-4 position	618:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	4	34	theme	O-4	644:646	arg1	position					648:655	O-4 position	644:655	O-4 position	644:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	3	35	theme	sugar	466:470	arg1	composition					472:482	sugar composition	466:482	sugar composition	466:482	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	3	36	theme	Monosaccharide	415:428	arg1	analysis					442:449	Monosaccharide composition analysis	415:449	Monosaccharide composition analysis	415:449	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	0	37	from	the	75:77	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	0	37	from	the	75:77	arg1	activity					39:46	antioxidant activity	27:46	antioxidant activity	27:46	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	0	37	from	the	75:77	arg1	arabinogalactan					54:68	an arabinogalactan	51:68	an arabinogalactan from the leaves of Moringa oleifera	51:104	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	0	38	theme	arabinogalactan	54:68	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	0	38	theme	arabinogalactan	54:68	arg1	activity					39:46	antioxidant activity	27:46	antioxidant activity	27:46	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	6	39	theme	ABTS	875:878	arg1	cation					889:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation	823:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	6	40	theme	scavenging	896:905	arg1	capacity					907:914	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	4	41	contain	has	576:578	arg2	backbone					582:589	a backbone	580:589	a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position	580:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	4	41	contain	has	576:578	arg1	MOP-1					570:574	MOP-1	570:574	MOP-1	570:574	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	1	42	theme	novel	109:113	arg1	MOP-1					132:136	MOP-1	132:136	MOP-1	132:136	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	1	42	theme	novel	109:113	arg1	arabinogalactan					115:129	A novel arabinogalactan	107:129	A novel arabinogalactan (MOP-1)	107:137	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	4	43	theme	Structural	535:544	arg1	analysis					546:553	Structural analysis	535:553	Structural analysis	535:553	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	6	44	theme	2,2'-Azinobis-	823:836	arg1	cation					889:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation	823:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	6	45	theme	DPPH	789:792	arg1	capacity					813:820	DPPH radical scavenging capacity	789:820	DPPH radical scavenging capacity	789:820	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	4	46	dep	-β-d-Galp-	597:606	arg1	→1					594:595	→1	594:595	→1	594:595	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	0	47	dep	the	75:77	arg1	leaves					79:84	leaves	79:84	leaves	79:84	Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.					
29366898	1	48	theme	macro-porous	204:215	arg1	resin					217:221	macro-porous resin	204:221	macro-porous resin	204:221	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
29366898	4	49	with	backbone	582:589	arg1	chains					634:639	highly branched chains	618:639	highly branched chains at O-4 position	618:655	Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position.					
29366898	2	50	theme	chromatography	322:335	arg1	analysis					345:352	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis	290:352	High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 107 Da.					
29366898	5	51	dep	1→	740:741	arg1	Araf-					724:728	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	Araf-	724:728	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	5	51	dep	1→	740:741	arg1	Galp-					734:738	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	Galp-	734:738	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	5	51	dep	1→	740:741	arg1	→1					706:707	→1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→	688:741	→1	706:707	The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→.					
29366898	3	52	theme	molar	506:510	arg1	ratio					512:516	a molar ratio	504:516	a molar ratio of 1:7.32:12.12	504:532	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	6	53	theme	3-ethylbenzthiazoline-6-sulphonate	838:871	arg1	cation					889:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation	823:894	2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity	823:914	Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP).					
29366898	3	54	dep	Rha	487:489	arg1	Gal					497:499	Gal	497:499	Gal	497:499	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	3	54	dep	Rha	487:489	arg1	Ara					492:494	Ara	492:494	Ara	492:494	Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12.					
29366898	1	55	theme	DEAE-52	224:230	arg1	Cellulose					232:240	DEAE-52 Cellulose	224:240	DEAE-52 Cellulose	224:240	A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography.					
30170051	0	0	theme	Lallemantia	87:97	arg1	seeds					106:110	Lallemantia ibrica seeds	87:110	Lallemantia ibrica seeds	87:110	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	8	1	theme	high	1117:1120	arg1	viscosity					1122:1130	its high viscosity	1113:1130	its high viscosity at low concentration	1113:1151	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	1	2	theme	emulsifying	163:173	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	2	theme	emulsifying	163:173	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	8	3	theme	shear	1077:1081	arg1	behavior					1092:1099	The shear thinning behavior	1073:1099	The shear thinning behavior of LISG	1073:1107	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	3	4	theme	6.52	611:614	arg1	%					615:615	%	615:615	%	615:615	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	5	theme	0.2	598:600	arg1	%					601:601	%	601:601	%	601:601	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	8	6	from	stabilizer	1168:1177	arg1	industry					1205:1212	the food industry	1196:1212	the food industry	1196:1212	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	3	7	theme	molecular	476:484	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	7	theme	molecular	476:484	arg1	5.74 × 106 g/mol					509:524	5.74 × 106 g/mol	509:524	5.74 × 106 g/mol	509:524	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	7	theme	molecular	476:484	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	6	8	theme	tested	880:885	arg1	concentrations					887:900	all tested concentrations	876:900	all tested concentrations	876:900	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	3	9	theme	seed	450:453	arg1	LISG					460:463	LISG	460:463	LISG	460:463	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	9	theme	seed	450:453	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	9	theme	seed	450:453	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	10	theme	%	615:615	arg1	moisture					617:624	6.52% moisture	611:624	6.52% moisture	611:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	10	theme	%	615:615	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	4	11	theme	functional	732:741	arg1	groups					743:748	O-acetyl functional groups	723:748	O-acetyl functional groups	723:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	4	12	theme	galactose	677:685	arg1	presence					665:672	the presence	661:672	the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups	661:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	0	13	theme	ibrica	99:104	arg1	seeds					106:110	Lallemantia ibrica seeds	87:110	Lallemantia ibrica seeds	87:110	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	8	14	theme	good	1163:1166	arg1	stabilizer					1168:1177	a good stabilizer	1161:1177	a good stabilizer	1161:1177	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	8	15	theme	food	1200:1203	arg1	industry					1205:1212	the food industry	1196:1212	the food industry	1196:1212	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	2	16	theme	extraction	354:363	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	16	theme	extraction	354:363	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	4	17	theme	1H	636:637	arg1	analysis					643:650	FTIR and 1H NMR analysis	627:650	analysis	643:650	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	5	18	from	25 °C	787:791	arg1	LISG					778:781	LISG	778:781	LISG (at 25 °C)	778:792	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	5	18	from	25 °C	787:791	arg1	viscosity					765:773	The intrinsic viscosity	751:773	The intrinsic viscosity of LISG (at 25 °C)	751:792	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	5	18	from	25 °C	787:791	arg1	1.96 dL/g					814:822	1.96 dL/g	814:822	1.96 dL/g	814:822	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	2	19	theme	highest	276:282	arg1	yield					284:288	yield	284:288	yield	284:288	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	5	20	theme	LISG	778:781	arg1	viscosity					765:773	The intrinsic viscosity	751:773	The intrinsic viscosity of LISG (at 25 °C)	751:792	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	5	20	theme	LISG	778:781	arg1	1.96 dL/g					814:822	1.96 dL/g	814:822	1.96 dL/g	814:822	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	1	21	theme	stabilizing	176:186	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	21	theme	stabilizing	176:186	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	4	22	theme	groups	743:748	arg1	presence					665:672	the presence	661:672	the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups	661:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	2	23	theme	pH = 10	390:396	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	23	theme	pH = 10	390:396	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	7	24	dep	water	1006:1010	arg1	pH = 7					1013:1018	pH = 7	1013:1018	pH = 7	1013:1018	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	1	25	theme	thickening	189:198	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	25	theme	thickening	189:198	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	7	26	theme	LISG	1052:1055	arg1	macromolecules					1057:1070	LISG macromolecules	1052:1070	LISG macromolecules	1052:1070	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	8	27	from	thickener	1183:1191	arg1	industry					1205:1212	the food industry	1196:1212	the food industry	1196:1212	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	1	28	used	used	141:144	arg2	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	28	used	used	141:144	arg2	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	2	29	theme	68.75 °C	380:387	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	29	theme	68.75 °C	380:387	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	30	theme	36:1	425:428	arg1	ratio					416:420	ratio	416:420	ratio of 36:1	416:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	3	31	theme	2.98	572:575	arg1	%					576:576	%	576:576	%	576:576	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	4	32	theme	uronic	707:712	arg1	acid					714:717	uronic acid	707:717	uronic acid	707:717	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	4	33	theme	O-acetyl	723:730	arg1	groups					743:748	O-acetyl functional groups	723:748	O-acetyl functional groups	723:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	2	34	theme	carbohydrate	300:311	arg1	content					313:319	total carbohydrate content	294:319	total carbohydrate content	294:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	7	35	theme	deionized	996:1004	arg1	solvent					1040:1046	a good solvent	1033:1046	a good solvent for LISG macromolecules	1033:1070	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	7	35	theme	deionized	996:1004	arg1	water					1006:1010	deionized water	996:1010	deionized water (pH = 7 at 25 °C)	996:1028	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	1	36	theme	gelling	201:207	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	36	theme	gelling	201:207	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	8	37	from	concentration	1139:1151	arg1	behavior					1092:1099	The shear thinning behavior	1073:1099	The shear thinning behavior of LISG	1073:1107	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	8	37	from	concentration	1139:1151	arg1	viscosity					1122:1130	its high viscosity	1113:1130	its high viscosity at low concentration	1113:1151	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	4	38	theme	mannose	688:694	arg1	presence					665:672	the presence	661:672	the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups	661:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	2	39	theme	total	294:298	arg1	content					313:319	total carbohydrate content	294:319	total carbohydrate content	294:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	3	40	theme	weight	486:491	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	40	theme	weight	486:491	arg1	5.74 × 106 g/mol					509:524	5.74 × 106 g/mol	509:524	5.74 × 106 g/mol	509:524	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	40	theme	weight	486:491	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	41	theme	high	471:474	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	41	theme	high	471:474	arg1	5.74 × 106 g/mol					509:524	5.74 × 106 g/mol	509:524	5.74 × 106 g/mol	509:524	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	41	theme	high	471:474	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	42	theme	ibrica	443:448	arg1	LISG					460:463	LISG	460:463	LISG	460:463	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	42	theme	ibrica	443:448	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	42	theme	ibrica	443:448	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	6	43	contain	had	843:845	arg2	behavior					864:871	a shear-thinning behavior	847:871	a shear-thinning behavior	847:871	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	6	43	contain	had	843:845	arg1	solutions					833:841	The gum solutions	825:841	The gum solutions	825:841	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	6	44	theme	rheological	956:966	arg1	behavior					968:975	their rheological behavior	950:975	their rheological behavior	950:975	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	1	45	theme	binding	210:216	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	45	theme	binding	210:216	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	0	46	theme	rheological	33:43	arg1	study					45:49	rheological study	33:49	rheological study	33:49	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	8	47	theme	thinning	1083:1090	arg1	behavior					1092:1099	The shear thinning behavior	1073:1099	The shear thinning behavior of LISG	1073:1107	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	3	48	theme	8.95	587:590	arg1	%					591:591	%	591:591	%	591:591	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	4	49	theme	acid	714:717	arg1	presence					665:672	the presence	661:672	the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups	661:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	5	50	theme	intrinsic	755:763	arg1	viscosity					765:773	The intrinsic viscosity	751:773	The intrinsic viscosity of LISG (at 25 °C)	751:792	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	5	50	theme	intrinsic	755:763	arg1	1.96 dL/g					814:822	1.96 dL/g	814:822	1.96 dL/g	814:822	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	2	51	theme	water	402:406	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	51	theme	water	402:406	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	8	52	theme	LISG	1104:1107	arg1	behavior					1092:1099	The shear thinning behavior	1073:1099	The shear thinning behavior of LISG	1073:1107	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	8	52	theme	LISG	1104:1107	arg1	viscosity					1122:1130	its high viscosity	1113:1130	its high viscosity at low concentration	1113:1151	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	6	53	theme	gum	829:831	arg1	solutions					833:841	The gum solutions	825:841	The gum solutions	825:841	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	6	54	theme	model	921:925	arg1	satisfactory					927:938	the Power-law model satisfactory	907:938	the Power-law model satisfactory	907:938	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	3	55	theme	%	591:591	arg1	ash					593:595	8.95% ash	587:595	8.95% ash	587:595	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	55	theme	%	591:591	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	56	theme	89.60	551:555	arg1	%					556:556	%	556:556	%	556:556	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	2	57	theme	protein	342:348	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	57	theme	protein	342:348	arg1	protein					342:348	protein	342:348	protein	342:348	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	57	theme	protein	342:348	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	57	theme	protein	342:348	arg1	amount					332:337	lowest amount	325:337	lowest amount of protein	325:348	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	3	58	contain	containing	527:536	arg1	carbohydrate					558:569	89.60% carbohydrate	551:569	89.60% carbohydrate	551:569	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg1	moisture					617:624	6.52% moisture	611:624	6.52% moisture	611:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg2	5.74 × 106 g/mol					509:524	5.74 × 106 g/mol	509:524	5.74 × 106 g/mol	509:524	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg1	ash					593:595	8.95% ash	587:595	8.95% ash	587:595	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg2	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg2	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg1	protein					578:584	2.98% protein	572:584	2.98% protein	572:584	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	58	contain	containing	527:536	arg1	fat					603:605	0.2% fat	598:605	0.2% fat	598:605	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	59	theme	%	556:556	arg1	carbohydrate					558:569	89.60% carbohydrate	551:569	89.60% carbohydrate	551:569	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	59	theme	%	556:556	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	4	60	theme	FTIR	627:630	arg1	analysis					643:650	FTIR and 1H NMR analysis	627:650	analysis	643:650	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	3	61	theme	%	576:576	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	61	theme	%	576:576	arg1	protein					578:584	2.98% protein	572:584	2.98% protein	572:584	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	4	62	theme	rhamnose	697:704	arg1	presence					665:672	the presence	661:672	the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups	661:748	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	4	63	theme	NMR	639:641	arg1	analysis					643:650	FTIR and 1H NMR analysis	627:650	analysis	643:650	FTIR and 1H NMR analysis revealed the presence of galactose, mannose, rhamnose, uronic acid and O-acetyl functional groups.					
30170051	1	64	theme	coating	222:228	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	64	theme	coating	222:228	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	0	65	from	seeds	106:110	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	0	65	from	seeds	106:110	arg1	study					45:49	rheological study	33:49	rheological study	33:49	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	0	65	from	seeds	106:110	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	2	66	theme	lowest	325:330	arg1	protein					342:348	protein	342:348	protein	342:348	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	66	theme	lowest	325:330	arg1	amount					332:337	lowest amount	325:337	lowest amount of protein	325:348	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	6	67	theme	shear-thinning	849:862	arg1	behavior					864:871	a shear-thinning behavior	847:871	a shear-thinning behavior	847:871	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30170051	0	68	theme	polysaccharide	67:80	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	0	68	theme	polysaccharide	67:80	arg1	study					45:49	rheological study	33:49	rheological study	33:49	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	0	68	theme	polysaccharide	67:80	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	3	69	theme	%	601:601	arg1	average					542:548	average	542:548	average	542:548	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	69	theme	%	601:601	arg1	fat					603:605	0.2% fat	598:605	0.2% fat	598:605	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	2	70	theme	extraction	250:259	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	70	theme	extraction	250:259	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	8	71	theme	low	1135:1137	arg1	concentration					1139:1151	low concentration	1135:1151	low concentration	1135:1151	The shear thinning behavior of LISG and its high viscosity at low concentration make it a good stabilizer and thickener in the food industry.					
30170051	1	72	theme	plasticizing	149:160	arg1	agents					230:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents	149:235	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	1	72	theme	plasticizing	149:160	arg1	Polysaccharides					113:127	Polysaccharides	113:127	Polysaccharides	113:127	Polysaccharides are broadly used as plasticizing, emulsifying, stabilizing, thickening, gelling, binding and coating agents.					
30170051	3	73	theme	Lallemantia	431:441	arg1	LISG					460:463	LISG	460:463	LISG	460:463	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	73	theme	Lallemantia	431:441	arg1	gum					455:457	Lallemantia ibrica seed gum	431:457	Lallemantia ibrica seed gum (LISG)	431:464	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	3	73	theme	Lallemantia	431:441	arg1	polysaccharide					493:506	a high molecular weight polysaccharide	469:506	a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture	469:624	Lallemantia ibrica seed gum (LISG) is a high molecular weight polysaccharide (5.74 × 106 g/mol) containing, on average, 89.60% carbohydrate, 2.98% protein, 8.95% ash, 0.2% fat and 6.52% moisture.					
30170051	7	74	theme	good	1035:1038	arg1	solvent					1040:1046	a good solvent	1033:1046	a good solvent for LISG macromolecules	1033:1070	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	7	74	theme	good	1035:1038	arg1	water					1006:1010	deionized water	996:1010	deionized water (pH = 7 at 25 °C)	996:1028	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	0	75	theme	purified	58:65	arg1	polysaccharide					67:80	the purified polysaccharide	54:80	the purified polysaccharide	54:80	Extraction, characterization and rheological study of the purified polysaccharide from Lallemantia ibrica seeds.					
30170051	7	76	from	25 °C	1023:1027	arg1	pH = 7					1013:1018	pH = 7	1013:1018	pH = 7	1013:1018	It was found that deionized water (pH = 7 at 25 °C) is a good solvent for LISG macromolecules.					
30170051	2	77	theme	optimum	242:248	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1	354:428	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	2	77	theme	optimum	242:248	arg1	point					261:265	The optimum extraction point	238:265	The optimum extraction point based on highest yield and total carbohydrate content	238:319	The optimum extraction point based on highest yield and total carbohydrate content and lowest amount of protein was extraction temperature of 68.75 °C, pH = 10 and water to seed ratio of 36:1.					
30170051	5	78	from	viscosity	765:773	arg1	25 °C					787:791	25 °C	787:791	25 °C	787:791	The intrinsic viscosity of LISG (at 25 °C) was estimated to be 1.96 dL/g.					
30170051	6	79	theme	Power-law	911:919	arg1	satisfactory					927:938	the Power-law model satisfactory	907:938	the Power-law model satisfactory	907:938	The gum solutions had a shear-thinning behavior at all tested concentrations, and the Power-law model satisfactory described their rheological behavior.					
30042003	0	0	theme	isolated	90:97	arg1	cibaria					109:115	a newly isolated Weissella cibaria	82:115	a newly isolated Weissella cibaria under sucrose effect	82:136	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	6	1	theme	linkage	1012:1018	arg1	dextran					984:990	dextran	984:990	dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	984:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	1	theme	linkage	1012:1018	arg1	composition					962:972	The major composition	952:972	The major composition of EPS	952:979	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	2	theme	EPS	977:979	arg1	dextran					984:990	dextran	984:990	dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	984:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	2	theme	EPS	977:979	arg1	composition					962:972	The major composition	952:972	The major composition of EPS	952:979	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	3	3	theme	sucrose	605:611	arg1	60 g/L					595:600	60 g/L	595:600	60 g/L of sucrose	595:611	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	2	4	theme	sucrose	325:331	arg1	effects					314:320	the effects	310:320	the effects of sucrose	310:331	This is first-attempt to understand the effects of sucrose and achieve the highest exopolysaccharide (EPS) productivity from W27.					
30042003	8	5	theme	new	1303:1305	arg1	strain					1325:1330	this new dextran producing strain	1298:1330	this new dextran producing strain	1298:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	5	6	theme	optimal	899:905	arg1	conditions					907:916	optimal conditions	899:916	optimal conditions	899:916	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	6	7	theme	nuclear	1061:1067	arg1	resonance					1078:1086	nuclear magnetic resonance	1061:1086	nuclear magnetic resonance (NMR)	1061:1092	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	7	theme	nuclear	1061:1067	arg1	HPSEC					1146:1150	HPSEC	1146:1150	HPSEC	1146:1150	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	7	theme	nuclear	1061:1067	arg1	NMR					1089:1091	NMR	1089:1091	NMR	1089:1091	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	0	8	theme	Weissella	99:107	arg1	cibaria					109:115	a newly isolated Weissella cibaria	82:115	a newly isolated Weissella cibaria under sucrose effect	82:136	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	6	9	with	linkage	1012:1018	arg1	weight					1035:1040	molecular weight	1025:1040	molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1025:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	7	10	theme	surface	1167:1173	arg1	property					1175:1182	The surface property	1163:1182	The surface property of W27 induced by sucrose	1163:1208	The surface property of W27 induced by sucrose is become more hydrophobic to better inhibit bacteria.					
30042003	5	11	from	pH	935:936	arg1	pH					886:887	temperature > time > initial pH	857:887	temperature > time > initial pH	857:887	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	4	12	theme	cell	664:667	arg1	length					669:674	cell length	664:674	cell length changing shorter after sucrose addition	664:714	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	0	13	theme	sucrose	123:129	arg1	effect					131:136	sucrose effect	123:136	sucrose effect	123:136	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	1	14	from	fermentation	260:271	arg1	bacterium					187:195	a new lactic acid bacterium	169:195	a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation	169:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	14	from	fermentation	260:271	arg1	cibaria					149:155	Weissella cibaria 27	139:158	Weissella cibaria 27 (W27)	139:164	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	14	from	fermentation	260:271	arg1	important					241:249	important	241:249	important	241:249	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	2	15	theme	exopolysaccharide	357:373	arg1	productivity					381:392	the highest exopolysaccharide (EPS) productivity	345:392	the highest exopolysaccharide (EPS) productivity from W27	345:401	This is first-attempt to understand the effects of sucrose and achieve the highest exopolysaccharide (EPS) productivity from W27.					
30042003	3	16	theme	metabolic	408:416	arg1	compounds					418:426	The metabolic compounds	404:426	The metabolic compounds of lactic acid, acetic acid and ethanol	404:466	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	8	17	theme	cultural	1276:1283	arg1	approach					1285:1292	The simple cultural approach	1265:1292	The simple cultural approach for this new dextran producing strain	1265:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	8	17	theme	cultural	1276:1283	arg1	W27					1333:1335	W27	1333:1335	W27	1333:1335	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	5	18	from	22°C	926:929	arg1	pH					886:887	temperature > time > initial pH	857:887	temperature > time > initial pH	857:887	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	5	19	theme	L9	743:744	arg1	array					757:761	an L9 orthogonal array	740:761	an L9 orthogonal array	740:761	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	1	20	theme	new	171:173	arg1	bacterium					187:195	a new lactic acid bacterium	169:195	a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation	169:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	20	theme	new	171:173	arg1	cibaria					149:155	Weissella cibaria 27	139:158	Weissella cibaria 27 (W27)	139:164	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	20	theme	new	171:173	arg1	important					241:249	important	241:249	important	241:249	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	5	21	theme	orthogonal	746:755	arg1	array					757:761	an L9 orthogonal array	740:761	an L9 orthogonal array	740:761	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	6	22	theme	resonance	1078:1086	arg1	analysis					1153:1160	nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1061:1160	nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1061:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	2	23	theme	highest	349:355	arg1	productivity					381:392	the highest exopolysaccharide (EPS) productivity	345:392	the highest exopolysaccharide (EPS) productivity from W27	345:401	This is first-attempt to understand the effects of sucrose and achieve the highest exopolysaccharide (EPS) productivity from W27.					
30042003	1	24	theme	diary	254:258	arg1	fermentation					260:271	diary fermentation	254:271	diary fermentation	254:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	5	25	theme	temperature > time > initial	857:884	arg1	pH					886:887	temperature > time > initial pH	857:887	temperature > time > initial pH	857:887	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	4	26	theme	sucrose	699:705	arg1	addition					707:714	sucrose addition	699:714	sucrose addition	699:714	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	1	27	theme	acid	182:185	arg1	bacterium					187:195	a new lactic acid bacterium	169:195	a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation	169:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	27	theme	acid	182:185	arg1	cibaria					149:155	Weissella cibaria 27	139:158	Weissella cibaria 27 (W27)	139:164	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	27	theme	acid	182:185	arg1	important					241:249	important	241:249	important	241:249	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	8	28	contain	has	1338:1340	arg2	potential					1342:1350	potential	1342:1350	potential	1342:1350	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	8	28	contain	has	1338:1340	arg1	approach					1285:1292	The simple cultural approach	1265:1292	The simple cultural approach for this new dextran producing strain	1265:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	8	28	contain	has	1338:1340	arg1	W27					1333:1335	W27	1333:1335	W27	1333:1335	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	1	29	from	important	241:249	arg1	fermentation					260:271	diary fermentation	254:271	diary fermentation	254:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	6	30	theme	glycosidic	1001:1010	arg1	linkage					1012:1018	α-1,6 glycosidic linkage	995:1018	α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	995:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	31	theme	molecular	1025:1033	arg1	weight					1035:1040	molecular weight	1025:1040	molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1025:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	4	32	theme	electron	623:630	arg1	SEM					644:646	SEM	644:646	SEM	644:646	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	4	32	theme	electron	623:630	arg1	microscopy					632:641	Scanning electron microscopy	614:641	Scanning electron microscopy (SEM) results	614:655	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	6	33	theme	1.2 x 107 Da	1045:1056	arg1	weight					1035:1040	molecular weight	1025:1040	molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1025:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	1	34	theme	lactic	175:180	arg1	bacterium					187:195	a new lactic acid bacterium	169:195	a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation	169:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	34	theme	lactic	175:180	arg1	cibaria					149:155	Weissella cibaria 27	139:158	Weissella cibaria 27 (W27)	139:164	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	34	theme	lactic	175:180	arg1	important					241:249	important	241:249	important	241:249	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	0	35	theme	antibacterial	33:45	arg1	activity					47:54	antibacterial activity	33:54	antibacterial activity	33:54	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	5	36	theme	culture	802:808	arg1	effects					791:797	the effects	787:797	the effects of culture	787:808	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	8	37	theme	producing	1315:1323	arg1	strain					1325:1330	this new dextran producing strain	1298:1330	this new dextran producing strain	1298:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	3	38	theme	lactic	431:436	arg1	acid					438:441	lactic acid	431:441	lactic acid	431:441	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	0	39	theme	exopolysaccharide	59:75	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	0	39	theme	exopolysaccharide	59:75	arg1	activity					47:54	antibacterial activity	33:54	antibacterial activity	33:54	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	0	39	theme	exopolysaccharide	59:75	arg1	Production					0:9	Production	0:9	Production	0:9	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	8	40	theme	simple	1269:1274	arg1	approach					1285:1292	The simple cultural approach	1265:1292	The simple cultural approach for this new dextran producing strain	1265:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	8	40	theme	simple	1269:1274	arg1	W27					1333:1335	W27	1333:1335	W27	1333:1335	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	5	41	theme	EPS	839:841	arg1	production					843:852	the EPS production	835:852	the EPS production	835:852	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	8	42	dep	food	1359:1362	arg1	the					1355:1357	the	1355:1357	the	1355:1357	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	8	43	theme	antibacterial	1371:1383	arg1	agent					1385:1389	antibacterial agent	1371:1389	antibacterial agent	1371:1389	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	3	44	theme	ethanol	460:466	arg1	compounds					418:426	The metabolic compounds	404:426	The metabolic compounds of lactic acid, acetic acid and ethanol	404:466	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	7	45	theme	W27	1187:1189	arg1	property					1175:1182	The surface property	1163:1182	The surface property of W27 induced by sucrose	1163:1208	The surface property of W27 induced by sucrose is become more hydrophobic to better inhibit bacteria.					
30042003	4	46	theme	Scanning	614:621	arg1	SEM					644:646	SEM	644:646	SEM	644:646	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	4	46	theme	Scanning	614:621	arg1	microscopy					632:641	Scanning electron microscopy	614:641	Scanning electron microscopy (SEM) results	614:655	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30042003	5	47	dep	evaluate	778:785	arg1	ranked					815:820	ranked	815:820	were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h	810:949	By Taguchi's approach, an L9 orthogonal array was adopted to evaluate the effects of culture were ranked according to the EPS production as temperature > time > initial pH, in which optimal conditions were at 22°C and pH 6.2 for 24 h.					
30042003	1	48	theme	Weissella	139:147	arg1	bacterium					187:195	a new lactic acid bacterium	169:195	a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation	169:271	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	48	theme	Weissella	139:147	arg1	cibaria					149:155	Weissella cibaria 27	139:158	Weissella cibaria 27 (W27)	139:164	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	48	theme	Weissella	139:147	arg1	W27					161:163	W27	161:163	W27	161:163	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	1	48	theme	Weissella	139:147	arg1	important					241:249	important	241:249	important	241:249	Weissella cibaria 27 (W27) is a new lactic acid bacterium which has been screened from kimchi, and is important in diary fermentation.					
30042003	6	49	theme	magnetic	1069:1076	arg1	resonance					1078:1086	nuclear magnetic resonance	1061:1086	nuclear magnetic resonance (NMR)	1061:1092	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	49	theme	magnetic	1069:1076	arg1	HPSEC					1146:1150	HPSEC	1146:1150	HPSEC	1146:1150	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	49	theme	magnetic	1069:1076	arg1	NMR					1089:1091	NMR	1089:1091	NMR	1089:1091	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	3	50	dep	24.8 g/L	581:588	arg1	up					575:576	up	575:576	up	575:576	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	0	51	from	cibaria	109:115	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	0	51	from	cibaria	109:115	arg1	activity					47:54	antibacterial activity	33:54	antibacterial activity	33:54	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	0	51	from	cibaria	109:115	arg1	Production					0:9	Production	0:9	Production	0:9	Production, characterization and antibacterial activity of exopolysaccharide from a newly isolated Weissella cibaria under sucrose effect.					
30042003	6	52	theme	high-performance	1098:1113	arg1	chromatography					1130:1143	high-performance size-exclusion chromatography	1098:1143	high-performance size-exclusion chromatography	1098:1143	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	3	53	theme	acid	451:454	arg1	compounds					418:426	The metabolic compounds	404:426	The metabolic compounds of lactic acid, acetic acid and ethanol	404:466	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	3	54	theme	acetic	444:449	arg1	acid					451:454	acetic acid	444:454	acetic acid	444:454	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	6	55	theme	major	956:960	arg1	dextran					984:990	dextran	984:990	dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	984:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	55	theme	major	956:960	arg1	composition					962:972	The major composition	952:972	The major composition of EPS	952:979	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	6	56	theme	chromatography	1130:1143	arg1	analysis					1153:1160	nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1061:1160	nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis	1061:1160	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	3	57	theme	acid	438:441	arg1	compounds					418:426	The metabolic compounds	404:426	The metabolic compounds of lactic acid, acetic acid and ethanol	404:466	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	3	58	theme	similar	475:481	arg1	levels					483:488	similar levels	475:488	similar levels	475:488	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	8	59	theme	dextran	1307:1313	arg1	strain					1325:1330	this new dextran producing strain	1298:1330	this new dextran producing strain	1298:1330	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	3	60	with	cultures	499:506	arg1	lactose					524:530	lactose	524:530	lactose	524:530	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	3	60	with	cultures	499:506	arg1	glucose					513:519	glucose	513:519	glucose	513:519	The metabolic compounds of lactic acid, acetic acid and ethanol are at similar levels when the cultures with glucose or lactose; except for EPS is significantly increased up to 24.8 g/L with 60 g/L of sucrose.					
30042003	8	61	theme	cosmetic	1396:1403	arg1	industry					1405:1412	cosmetic industry	1396:1412	cosmetic industry	1396:1412	The simple cultural approach for this new dextran producing strain, W27, has potential in the food, feed, antibacterial agent, and cosmetic industry.					
30042003	6	62	theme	size-exclusion	1115:1128	arg1	chromatography					1130:1143	high-performance size-exclusion chromatography	1098:1143	high-performance size-exclusion chromatography	1098:1143	The major composition of EPS is dextran of α-1,6 glycosidic linkage with molecular weight of 1.2 x 107 Da by nuclear magnetic resonance (NMR) and high-performance size-exclusion chromatography (HPSEC) analysis.					
30042003	2	63	from	W27	399:401	arg1	productivity					381:392	the highest exopolysaccharide (EPS) productivity	345:392	the highest exopolysaccharide (EPS) productivity from W27	345:401	This is first-attempt to understand the effects of sucrose and achieve the highest exopolysaccharide (EPS) productivity from W27.					
30042003	4	64	theme	microscopy	632:641	arg1	results					649:655	Scanning electron microscopy (SEM) results	614:655	Scanning electron microscopy (SEM) results	614:655	Scanning electron microscopy (SEM) results reveal cell length changing shorter after sucrose addition.					
30986466	0	0	theme	Dioscorea	79:87	arg1	Thunb					98:102	Dioscorea opposita Thunb	79:102	Dioscorea opposita Thunb	79:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	1	from	structure	11:19	arg1	Thunb					98:102	Dioscorea opposita Thunb	79:102	Dioscorea opposita Thunb	79:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	5	2	dep	-β-d-gal	881:888	arg1	-α-d-glc					832:839	→4)-α-d-glc	829:839	→4)-α-d-glc	829:839	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	5	2	dep	-β-d-gal	881:888	arg1	1 → residues					841:852	1 → residues	841:852	1 → residues	841:852	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	4	3	theme	DOTP-B	558:563	arg1	features					546:553	Structural features	535:553	Structural features of DOTP-B	535:563	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	5	4	theme	internal	869:876	arg1	→6					878:879	about 6% internal →6	860:879	about 6% internal →6	860:879	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	7	5	theme	polysaccharide	1079:1092	arg1	DOTP-B					1094:1099	the polysaccharide DOTP-B	1075:1099	the polysaccharide DOTP-B	1075:1099	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	7	5	theme	polysaccharide	1079:1092	arg1	antioxidant					1152:1162	a promising natural antioxidant	1132:1162	a promising natural antioxidant for application in medical and food industries	1132:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	4	6	theme	periodate	676:684	arg1	oxidation					686:694	periodate oxidation	676:694	periodate oxidation	676:694	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	0	7	from	Isolation	0:8	arg1	Thunb					98:102	Dioscorea opposita Thunb	79:102	Dioscorea opposita Thunb	79:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	5	8	dep	→6	878:879	arg1	%					867:867	%	867:867	%	867:867	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	1	9	theme	well-known	240:249	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	9	theme	well-known	240:249	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	0	10	theme	opposita	89:96	arg1	Thunb					98:102	Dioscorea opposita Thunb	79:102	Dioscorea opposita Thunb	79:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	2	11	from	neutral	311:317	arg1	charge					322:327	charge	322:327	charge	322:327	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	4	12	theme	NMR	734:736	arg1	spectra					738:744	several NMR spectra	726:744	several NMR spectra	726:744	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	1	13	theme	edible	251:256	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	13	theme	edible	251:256	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	7	14	theme	natural	1144:1150	arg1	DOTP-B					1094:1099	the polysaccharide DOTP-B	1075:1099	the polysaccharide DOTP-B	1075:1099	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	7	14	theme	natural	1144:1150	arg1	antioxidant					1152:1162	a promising natural antioxidant	1132:1162	a promising natural antioxidant for application in medical and food industries	1132:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	1	15	from	plant	272:276	arg1	China					281:285	China	281:285	China	281:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	5	16	dep	%	867:867	arg1	6					866:866	6	866:866	6	866:866	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	3	17	theme	composition	390:400	arg1	analysis					402:409	Monosaccharide composition analysis	375:409	Monosaccharide composition analysis by GC-MS	375:418	Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.6:1.0.					
30986466	6	18	theme	DOTP-B	932:937	arg1	activity					920:927	The antioxidant activity	904:927	The antioxidant activity of DOTP-B	904:937	The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 ± 0.1 mg/mL and 1.6 ± 0.1 mg/mL, respectively.					
30986466	2	19	from	charge	322:327	arg1	neutral					311:317	neutral	311:317	neutral	311:317	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	1	20	dep	unknown	118:124	arg1	water-soluble					127:139	water-soluble	127:139	water-soluble	127:139	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	2	21	theme	molecular	346:354	arg1	weight					356:361	an average molecular weight	335:361	an average molecular weight of 5623 Da	335:372	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	0	22	from	activity	25:32	arg1	Thunb					98:102	Dioscorea opposita Thunb	79:102	Dioscorea opposita Thunb	79:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	7	23	theme	medical	1183:1189	arg1	industries					1200:1209	medical and food industries	1183:1209	medical and food industries	1183:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	5	24	theme	-β-d-gal	881:888	arg1	1 → residues					890:901	→4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues	829:901	→4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues	829:901	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	2	25	theme	average	338:344	arg1	weight					356:361	an average molecular weight	335:361	an average molecular weight of 5623 Da	335:372	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	5	26	theme	main	795:798	arg1	chain					800:804	the main chain	791:804	the main chain of DOTP-B	791:814	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	4	27	theme	complete	650:657	arg1	hydrolysis					664:673	complete acid hydrolysis	650:673	complete acid hydrolysis	650:673	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	1	28	theme	medicinal	262:270	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	28	theme	medicinal	262:270	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	3	29	theme	14.6:1.0	525:532	arg1	ratio					516:520	a molar ratio	508:520	a molar ratio of 14.6:1.0	508:532	Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.6:1.0.					
30986466	4	30	theme	Structural	535:544	arg1	features					546:553	Structural features	535:553	Structural features of DOTP-B	535:563	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	6	31	theme	antioxidant	908:918	arg1	activity					920:927	The antioxidant activity	904:927	The antioxidant activity of DOTP-B	904:937	The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 ± 0.1 mg/mL and 1.6 ± 0.1 mg/mL, respectively.					
30986466	4	32	theme	chemical	605:612	arg1	methylation					697:707	methylation	697:707	methylation	697:707	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	GC-MS					710:714	GC-MS	710:714	GC-MS	710:714	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	spectra					738:744	several NMR spectra	726:744	several NMR spectra	726:744	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	hydrolysis					664:673	complete acid hydrolysis	650:673	complete acid hydrolysis	650:673	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	methods					631:637	chemical and instrumental methods	605:637	chemical and instrumental methods	605:637	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	oxidation					686:694	periodate oxidation	676:694	periodate oxidation	676:694	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	32	theme	chemical	605:612	arg1	FTIR					717:720	FTIR	717:720	FTIR	717:720	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	5	33	theme	correlated	754:763	arg1	results					765:771	Highly correlated results	747:771	Highly correlated results	747:771	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	4	34	theme	acid	659:662	arg1	hydrolysis					664:673	complete acid hydrolysis	650:673	complete acid hydrolysis	650:673	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	35	theme	methods	631:637	arg1	combination					590:600	a combination	588:600	a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra	588:744	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	7	36	from	application	1168:1178	arg1	industries					1200:1209	medical and food industries	1183:1209	medical and food industries	1183:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	2	37	theme	5623 Da	366:372	arg1	weight					356:361	an average molecular weight	335:361	an average molecular weight of 5623 Da	335:372	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	3	38	theme	Monosaccharide	375:388	arg1	analysis					402:409	Monosaccharide composition analysis	375:409	Monosaccharide composition analysis by GC-MS	375:418	Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.6:1.0.					
30986466	2	39	with	neutral	311:317	arg1	weight					356:361	an average molecular weight	335:361	an average molecular weight of 5623 Da	335:372	DOTP-B was found to be neutral in charge, with an average molecular weight of 5623 Da.					
30986466	4	40	theme	instrumental	618:629	arg1	methylation					697:707	methylation	697:707	methylation	697:707	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	GC-MS					710:714	GC-MS	710:714	GC-MS	710:714	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	spectra					738:744	several NMR spectra	726:744	several NMR spectra	726:744	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	hydrolysis					664:673	complete acid hydrolysis	650:673	complete acid hydrolysis	650:673	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	methods					631:637	chemical and instrumental methods	605:637	chemical and instrumental methods	605:637	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	oxidation					686:694	periodate oxidation	676:694	periodate oxidation	676:694	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	4	40	theme	instrumental	618:629	arg1	FTIR					717:720	FTIR	717:720	FTIR	717:720	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	0	41	theme	novel	39:43	arg1	polysaccharide					59:72	a novel water-soluble polysaccharide	37:72	a novel water-soluble polysaccharide from Dioscorea opposita Thunb	37:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	1	42	attach	isolated	175:182	arg1	roots					193:197	the roots	189:197	the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China	189:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	42	attach	isolated	175:182	arg2	polysaccharide					141:154	A previously unknown, water-soluble polysaccharide	105:154	A previously unknown, water-soluble polysaccharide	105:154	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	5	43	with	1 → residues	841:852	arg1	→6					878:879	about 6% internal →6	860:879	about 6% internal →6	860:879	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	5	44	dep	-α-d-glc	832:839	arg1	→4					829:830	→4	829:830	→4	829:830	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	1	45	theme	unknown	118:124	arg1	polysaccharide					141:154	A previously unknown, water-soluble polysaccharide	105:154	A previously unknown, water-soluble polysaccharide	105:154	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	6	46	theme	EC50	965:968	arg1	2.1 ± 0.1 mg/mL					1013:1027	2.1 ± 0.1 mg/mL	1013:1027	2.1 ± 0.1 mg/mL	1013:1027	The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 ± 0.1 mg/mL and 1.6 ± 0.1 mg/mL, respectively.					
30986466	6	46	theme	EC50	965:968	arg1	values					970:975	the EC50 values	961:975	the EC50 values against DPPH and PTIO radicals	961:1006	The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 ± 0.1 mg/mL and 1.6 ± 0.1 mg/mL, respectively.					
30986466	4	47	theme	several	726:732	arg1	spectra					738:744	several NMR spectra	726:744	several NMR spectra	726:744	Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra.					
30986466	1	48	theme	plant	206:210	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	48	theme	plant	206:210	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	3	49	theme	molar	510:514	arg1	ratio					516:520	a molar ratio	508:520	a molar ratio of 14.6:1.0	508:532	Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.6:1.0.					
30986466	0	50	from	Thunb	98:102	arg1	structure					11:19	structure	11:19	structure	11:19	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	50	from	Thunb	98:102	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	50	from	Thunb	98:102	arg1	polysaccharide					59:72	a novel water-soluble polysaccharide	37:72	a novel water-soluble polysaccharide from Dioscorea opposita Thunb	37:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	50	from	Thunb	98:102	arg1	activity					25:32	activity	25:32	activity	25:32	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	6	51	theme	PTIO	994:997	arg1	radicals					999:1006	PTIO radicals	994:1006	PTIO radicals	994:1006	The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 ± 0.1 mg/mL and 1.6 ± 0.1 mg/mL, respectively.					
30986466	1	52	theme	Dioscorea	212:220	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	52	theme	Dioscorea	212:220	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	0	53	theme	polysaccharide	59:72	arg1	structure					11:19	structure	11:19	structure	11:19	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	53	theme	polysaccharide	59:72	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	0	53	theme	polysaccharide	59:72	arg1	activity					25:32	activity	25:32	activity	25:32	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	5	54	theme	DOTP-B	809:814	arg1	chain					800:804	the main chain	791:804	the main chain of DOTP-B	791:814	Highly correlated results demonstrated that the main chain of DOTP-B consisted of →4)-α-d-glc(1 → residues, with about 6% internal →6)-β-d-gal(1 → residues.					
30986466	7	55	theme	food	1195:1198	arg1	industries					1200:1209	medical and food industries	1183:1209	medical and food industries	1183:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	1	56	theme	opposita	222:229	arg1	plant					272:276	a well-known edible and medicinal plant	238:276	a well-known edible and medicinal plant in China	238:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	1	56	theme	opposita	222:229	arg1	Thunb					231:235	the plant Dioscorea opposita Thunb	202:235	the plant Dioscorea opposita Thunb	202:235	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30986466	0	57	theme	water-soluble	45:57	arg1	polysaccharide					59:72	a novel water-soluble polysaccharide	37:72	a novel water-soluble polysaccharide from Dioscorea opposita Thunb	37:102	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.					
30986466	7	58	theme	promising	1134:1142	arg1	DOTP-B					1094:1099	the polysaccharide DOTP-B	1075:1099	the polysaccharide DOTP-B	1075:1099	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	7	58	theme	promising	1134:1142	arg1	antioxidant					1152:1162	a promising natural antioxidant	1132:1162	a promising natural antioxidant for application in medical and food industries	1132:1209	Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.					
30986466	1	59	theme	Thunb	231:235	arg1	roots					193:197	the roots	189:197	the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China	189:285	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China.					
30658789	0	0	theme	hemicellulosic	78:91	arg1	polysaccharides					93:107	the cell wall hemicellulosic polysaccharides	64:107	the cell wall hemicellulosic polysaccharides	64:107	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	4	1	from	desiccated	645:654	arg1	walls					621:625	Isolated cell walls	607:625	Isolated cell walls from hydrated and desiccated leaves of each species	607:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	1	2	theme	tissues	181:187	arg1	dehydration					155:165	Drought-induced dehydration	139:165	Drought-induced dehydration of vegetative tissues in lycopods	139:199	Drought-induced dehydration of vegetative tissues in lycopods affects growth and survival.					
30658789	0	3	theme	wall	73:76	arg1	polysaccharides					93:107	the cell wall hemicellulosic polysaccharides	64:107	the cell wall hemicellulosic polysaccharides	64:107	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	0	4	from	tolerance	12:20	arg1	plants					25:30	plants	25:30	plants	25:30	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	3	5	theme	desiccation-sensitive	532:552	arg1	species					554:560	two desiccation-sensitive species	528:560	two desiccation-sensitive species	528:560	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	4	6	theme	cell	616:619	arg1	walls					621:625	Isolated cell walls	607:625	Isolated cell walls from hydrated and desiccated leaves of each species	607:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	4	7	from	hydrated	632:639	arg1	walls					621:625	Isolated cell walls	607:625	Isolated cell walls from hydrated and desiccated leaves of each species	607:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	6	8	theme	cell	986:989	arg1	polysaccharides					1011:1025	cell wall hemicellulosic polysaccharides	986:1025	cell wall hemicellulosic polysaccharides	986:1025	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	5	9	dep	wall	882:885	arg1	composition					887:897	composition	887:897	composition	887:897	Our results demonstrate that desiccation induces substantial modifications in the cell wall composition and structure.					
30658789	5	9	dep	wall	882:885	arg1	structure					903:911	structure	903:911	structure	903:911	Our results demonstrate that desiccation induces substantial modifications in the cell wall composition and structure.					
30658789	3	10	dep	species	503:509	arg1	involvens					514:522	involvens	514:522	involvens	514:522	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	0	11	theme	polysaccharides	93:107	arg1	characterization					44:59	Structural characterization	33:59	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.	0:137	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	0	12	theme	Selaginella	118:128	arg1	species					130:136	three Selaginella species	112:136	three Selaginella species	112:136	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	0	13	from	characterization	44:59	arg1	species					130:136	three Selaginella species	112:136	three Selaginella species	112:136	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	4	14	theme	each	666:669	arg1	species					671:677	each species	666:677	each species	666:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	3	15	theme	leaf	460:463	arg1	wall					470:473	the leaf cell wall	456:473	the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	456:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	6	16	theme	structural	961:970	arg1	remodeling					972:981	structural remodeling	961:981	structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan	961:1099	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	6	16	theme	structural	961:970	arg1	process					1117:1123	an important process	1104:1123	an important process in order to mitigate desiccation stress in Selaginella	1104:1178	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	3	17	theme	structural	425:434	arg1	characterization					436:451	the structural characterization	421:451	the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	421:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	6	18	from	stress	1158:1163	arg1	Selaginella					1168:1178	Selaginella	1168:1178	Selaginella	1168:1178	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	2	19	theme	mechanisms	300:309	arg1	series					278:283	a series	276:283	a series of specialized mechanisms	276:309	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	3	20	theme	cell	465:468	arg1	wall					470:473	the leaf cell wall	456:473	the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	456:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	5	21	theme	substantial	844:854	arg1	modifications					856:868	substantial modifications	844:868	substantial modifications	844:868	Our results demonstrate that desiccation induces substantial modifications in the cell wall composition and structure.					
30658789	1	22	from	dehydration	155:165	arg1	lycopods					192:199	lycopods	192:199	lycopods	192:199	Drought-induced dehydration of vegetative tissues in lycopods affects growth and survival.					
30658789	4	23	theme	oligosaccharide	715:729	arg1	fragments					731:739	the resulting oligosaccharide fragments	701:739	the resulting oligosaccharide fragments	701:739	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	0	24	theme	Desiccation	0:10	arg1	tolerance					12:20	Desiccation tolerance	0:20	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.	0:137	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	4	25	theme	resulting	705:713	arg1	fragments					731:739	the resulting oligosaccharide fragments	701:739	the resulting oligosaccharide fragments	701:739	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	6	26	theme	acetylated	1076:1085	arg1	galactomannan					1087:1099	acetylated galactomannan	1076:1099	acetylated galactomannan	1076:1099	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	2	27	theme	vegetative	338:347	arg1	tissues					349:355	vegetative tissues	338:355	vegetative tissues	338:355	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	4	28	theme	Isolated	607:614	arg1	walls					621:625	Isolated cell walls	607:625	Isolated cell walls from hydrated and desiccated leaves of each species	607:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	4	29	dep	hydrated	632:639	arg1	leaves					656:661	leaves	656:661	leaves of each species	656:677	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	6	30	theme	hemicellulosic	996:1009	arg1	polysaccharides					1011:1025	cell wall hemicellulosic polysaccharides	986:1025	cell wall hemicellulosic polysaccharides	986:1025	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	3	31	theme	desiccation-tolerant	482:501	arg1	kraussiana					573:582	S. kraussiana	570:582	S. kraussiana	570:582	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	3	31	theme	desiccation-tolerant	482:501	arg1	moellendorffii					591:604	S. moellendorffii	588:604	S. moellendorffii	588:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	3	31	theme	desiccation-tolerant	482:501	arg1	species					503:509	the desiccation-tolerant species S. involvens and two desiccation-sensitive species	478:560	species	503:509	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	0	32	theme	Structural	33:42	arg1	characterization					44:59	Structural characterization	33:59	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.	0:137	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	3	33	theme	wall	470:473	arg1	characterization					436:451	the structural characterization	421:451	the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	421:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	6	34	theme	important	1107:1115	arg1	remodeling					972:981	structural remodeling	961:981	structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan	961:1099	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	6	34	theme	important	1107:1115	arg1	process					1117:1123	an important process	1104:1123	an important process in order to mitigate desiccation stress in Selaginella	1104:1178	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	6	35	theme	desiccation	1146:1156	arg1	stress					1158:1163	desiccation stress	1146:1163	desiccation stress in Selaginella	1146:1178	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	2	36	theme	Selaginella	251:261	arg1	species					240:246	Different species	230:246	Different species of Selaginella	230:261	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	5	37	theme	cell	877:880	arg1	wall					882:885	the cell wall composition and structure	873:911	the cell wall composition and structure	873:911	Our results demonstrate that desiccation induces substantial modifications in the cell wall composition and structure.					
30658789	6	38	theme	XXXG-rich	1037:1045	arg1	xyloglucan					1047:1056	XXXG-rich xyloglucan	1037:1056	XXXG-rich xyloglucan	1037:1056	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	6	39	theme	wall	991:994	arg1	polysaccharides					1011:1025	cell wall hemicellulosic polysaccharides	986:1025	cell wall hemicellulosic polysaccharides	986:1025	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	2	40	theme	water	372:376	arg1	stress					378:383	water stress	372:383	water stress	372:383	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	2	41	theme	Different	230:238	arg1	species					240:246	Different species	230:246	Different species of Selaginella	230:261	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	2	42	theme	specialized	288:298	arg1	mechanisms					300:309	specialized mechanisms	288:309	specialized mechanisms	288:309	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	1	43	theme	Drought-induced	139:153	arg1	dehydration					155:165	Drought-induced dehydration	139:165	Drought-induced dehydration of vegetative tissues in lycopods	139:199	Drought-induced dehydration of vegetative tissues in lycopods affects growth and survival.					
30658789	4	44	theme	structural	774:783	arg1	features					785:792	their structural features	768:792	their structural features	768:792	Isolated cell walls from hydrated and desiccated leaves of each species were fractionated and the resulting oligosaccharide fragments were analyzed to determine their structural features.					
30658789	2	45	from	desiccation	323:333	arg1	response					360:367	response	360:367	response to water stress	360:383	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	2	45	from	desiccation	323:333	arg1	tissues					349:355	vegetative tissues	338:355	vegetative tissues	338:355	Different species of Selaginella have evolved a series of specialized mechanisms to tolerate desiccation in vegetative tissues in response to water stress.					
30658789	3	46	theme	species	503:509	arg1	wall					470:473	the leaf cell wall	456:473	the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	456:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	0	47	theme	cell	68:71	arg1	polysaccharides					93:107	the cell wall hemicellulosic polysaccharides	64:107	the cell wall hemicellulosic polysaccharides	64:107	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	0	48	dep	tolerance	12:20	arg1	characterization					44:59	Structural characterization	33:59	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.	0:137	Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species.					
30658789	3	49	theme	species	554:560	arg1	wall					470:473	the leaf cell wall	456:473	the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii	456:604	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
30658789	6	50	theme	polysaccharides	1011:1025	arg1	remodeling					972:981	structural remodeling	961:981	structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan	961:1099	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	6	50	theme	polysaccharides	1011:1025	arg1	process					1117:1123	an important process	1104:1123	an important process in order to mitigate desiccation stress in Selaginella	1104:1178	Altogether, these data highlight the fact that structural remodeling of cell wall hemicellulosic polysaccharides including XXXG-rich xyloglucan, arabinoxylan and acetylated galactomannan is an important process in order to mitigate desiccation stress in Selaginella.					
30658789	1	51	theme	vegetative	170:179	arg1	tissues					181:187	vegetative tissues	170:187	vegetative tissues	170:187	Drought-induced dehydration of vegetative tissues in lycopods affects growth and survival.					
30658789	3	52	theme	present	393:399	arg1	study					401:405	the present study	389:405	the present study	389:405	In the present study, we report on the structural characterization of the leaf cell wall of the desiccation-tolerant species S. involvens and two desiccation-sensitive species, namely S. kraussiana and S. moellendorffii.					
29949660	5	0	theme	cell	1221:1224	arg1	polymers					1231:1238	different cell wall polymers	1211:1238	different cell wall polymers	1211:1238	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	5	1	theme	different	1052:1060	arg1	compositions					1069:1080	the different lignin compositions	1048:1080	the different lignin compositions	1048:1080	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	2	2	theme	lignin	346:351	arg1	compositions					353:364	different lignin compositions	336:364	different lignin compositions	336:364	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	6	3	theme	biotic	1428:1433	arg1	responses					1442:1450	biotic stress responses	1428:1450	biotic stress responses	1428:1450	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	6	4	theme	lignin	1255:1260	arg1	composition					1262:1272	lignin composition	1255:1272	lignin composition	1255:1272	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	3	5	theme	cis-jasmone-mediated	717:736	arg1	responses					738:746	cis-jasmone-mediated responses	717:746	cis-jasmone-mediated responses to aphids	717:756	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	4	6	theme	cell	929:932	arg1	walls					934:938	cell walls	929:938	cell walls of plants of the two lines	929:965	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	1	7	theme	ectopic	148:154	arg1	expression					156:165	the ectopic expression	144:165	the ectopic expression of defense genes	144:182	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	1	8	theme	such	237:240	arg1	phenomena					242:250	such phenomena	237:250	such phenomena	237:250	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	0	9	from	Elicitors	0:8	arg1	plants					40:45	plants	40:45	plants with altered lignin compositions	40:78	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	4	10	theme	plants	943:948	arg1	walls					934:938	cell walls	929:938	cell walls of plants of the two lines	929:965	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	2	11	theme	Arabidopsis	273:283	arg1	lines					285:289	Two Arabidopsis lines	269:289	Two Arabidopsis lines with similar lignin contents	269:318	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	5	12	theme	digestion	1015:1023	arg1	studies					1025:1031	Glycome profiling, fractionation and enzymatic digestion studies	968:1031	studies	1025:1031	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	5	13	theme	fractionation	987:999	arg1	studies					1025:1031	Glycome profiling, fractionation and enzymatic digestion studies	968:1031	studies	1025:1031	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	5	14	theme	elicitor	1176:1183	arg1	activity					1185:1192	elicitor activity	1176:1192	elicitor activity originating from different cell wall polymers	1176:1238	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	6	15	theme	defense	1385:1391	arg1	molecules					1400:1408	multiple latent defense signal molecules	1369:1408	multiple latent defense signal molecules	1369:1408	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	3	16	theme	oomycete	533:540	arg1	attack					565:570	oomycete and bacterial pathogen attack	533:570	oomycete and bacterial pathogen attack	533:570	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	17	theme	responsive	519:528	arg1	genes					513:517	genes	513:517	genes responsive to oomycete and bacterial pathogen attack	513:570	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	4	18	theme	water-soluble	901:913	arg1	extracts					915:922	water-soluble extracts	901:922	water-soluble extracts from cell walls of plants of the two lines	901:965	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	6	19	with	sequestration	1352:1364	arg1	impact					1418:1423	an impact	1415:1423	an impact on biotic stress responses	1415:1450	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	2	20	theme	different	377:385	arg1	responses					432:440	different quantitative and qualitative transcriptional responses	377:440	different quantitative and qualitative transcriptional responses	377:440	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	6	21	theme	wall	1311:1314	arg1	polysaccharides					1323:1337	plant cell wall matrix polysaccharides	1300:1337	plant cell wall matrix polysaccharides	1300:1337	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	6	22	theme	multiple	1369:1376	arg1	molecules					1400:1408	multiple latent defense signal molecules	1369:1408	multiple latent defense signal molecules	1369:1408	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	6	23	theme	composition	1262:1272	arg1	Alteration					1241:1250	Alteration	1241:1250	Alteration of lignin composition	1241:1272	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	2	24	theme	quantitative	387:398	arg1	responses					432:440	different quantitative and qualitative transcriptional responses	377:440	different quantitative and qualitative transcriptional responses	377:440	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	0	25	from	induction	27:35	arg1	plants					40:45	plants	40:45	plants with altered lignin compositions	40:78	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	2	26	theme	lignin	304:309	arg1	contents					311:318	similar lignin contents	296:318	similar lignin contents	296:318	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	1	27	theme	defense	170:176	arg1	genes					178:182	defense genes	170:182	defense genes	170:182	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	4	28	from	walls	934:938	arg1	extracts					915:922	water-soluble extracts	901:922	water-soluble extracts from cell walls of plants of the two lines	901:965	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	6	29	theme	cell	1306:1309	arg1	polysaccharides					1323:1337	plant cell wall matrix polysaccharides	1300:1337	plant cell wall matrix polysaccharides	1300:1337	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	2	30	theme	similar	296:302	arg1	contents					311:318	similar lignin contents	296:318	similar lignin contents	296:318	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	5	31	theme	wall	1226:1229	arg1	polymers					1231:1238	different cell wall polymers	1211:1238	different cell wall polymers	1211:1238	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	1	32	theme	genes	178:182	arg1	expression					156:165	the ectopic expression	144:165	the ectopic expression of defense genes	144:182	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	0	33	theme	gene	22:25	arg1	induction					27:35	defense gene induction	14:35	defense gene induction	14:35	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	3	34	theme	bacterial	808:816	arg1	inoculation					828:838	bacterial and aphid inoculation	808:838	bacterial and aphid inoculation	808:838	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	1	35	from	content	107:113	arg1	plants					129:134	transgenic plants	118:134	transgenic plants	118:134	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	5	36	theme	enzymatic	1005:1013	arg1	studies					1025:1031	Glycome profiling, fractionation and enzymatic digestion studies	968:1031	studies	1025:1031	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	3	37	theme	guaiacyl	484:491	arg1	units					493:497	guaiacyl units	484:497	guaiacyl units	484:497	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	0	38	theme	defense	14:20	arg1	induction					27:35	defense gene induction	14:35	defense gene induction	14:35	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	3	39	theme	altered	782:788	arg1	responsiveness					790:803	altered responsiveness	782:803	altered responsiveness to bacterial and aphid inoculation	782:838	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	40	theme	genes	679:683	arg1	some					696:699	some	696:699	some	696:699	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	40	theme	genes	679:683	arg1	number					661:666	a far greater number	647:666	a far greater number	647:666	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	2	41	theme	different	336:344	arg1	compositions					353:364	different lignin compositions	336:364	different lignin compositions	336:364	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	6	42	with	interactions	1282:1293	arg1	polysaccharides					1323:1337	plant cell wall matrix polysaccharides	1300:1337	plant cell wall matrix polysaccharides	1300:1337	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	5	43	theme	epitopes	1161:1168	arg1	range					1122:1126	a range	1120:1126	a range of heterogeneous oligosaccharide epitopes	1120:1168	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	2	44	theme	transcriptional	416:430	arg1	responses					432:440	different quantitative and qualitative transcriptional responses	377:440	different quantitative and qualitative transcriptional responses	377:440	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	6	45	theme	molecules	1400:1408	arg1	sequestration					1352:1364	the sequestration	1348:1364	the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses	1348:1450	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	2	46	with	lines	285:289	arg1	contents					311:318	similar lignin contents	296:318	similar lignin contents	296:318	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	4	47	theme	genes	864:868	arg1	Several					841:847	Several	841:847	Several	841:847	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	2	48	theme	qualitative	404:414	arg1	responses					432:440	different quantitative and qualitative transcriptional responses	377:440	different quantitative and qualitative transcriptional responses	377:440	Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses.					
29949660	5	49	theme	oligosaccharide	1145:1159	arg1	epitopes					1161:1168	heterogeneous oligosaccharide epitopes	1131:1168	heterogeneous oligosaccharide epitopes	1131:1168	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	6	50	from	impact	1418:1423	arg1	responses					1442:1450	biotic stress responses	1428:1450	biotic stress responses	1428:1450	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	3	51	with	Plants	443:448	arg1	lignin					455:460	lignin	455:460	lignin composed primarily of guaiacyl units	455:497	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	52	theme	defense	671:677	arg1	genes					679:683	defense genes	671:683	defense genes	671:683	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	6	53	theme	latent	1378:1383	arg1	molecules					1400:1408	multiple latent defense signal molecules	1369:1408	multiple latent defense signal molecules	1369:1408	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	1	54	from	importance	193:202	arg1	phenomena					242:250	such phenomena	237:250	such phenomena	237:250	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	1	55	theme	lignin	100:105	arg1	content					107:113	the lignin content	96:113	the lignin content in transgenic plants	96:134	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	3	56	with	plants	581:586	arg1	lignin					593:598	lignin	593:598	lignin composed primarily of syringyl units	593:635	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	57	theme	syringyl	622:629	arg1	units					631:635	syringyl units	622:635	syringyl units	622:635	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	5	58	theme	Glycome	968:974	arg1	studies					1025:1031	Glycome profiling, fractionation and enzymatic digestion studies	968:1031	studies	1025:1031	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	3	59	theme	aphid	822:826	arg1	inoculation					828:838	bacterial and aphid inoculation	808:838	bacterial and aphid inoculation	808:838	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	4	60	theme	lines	961:965	arg1	plants					943:948	plants	943:948	plants of the two lines	943:965	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	5	61	theme	range	1122:1126	arg1	extractability					1102:1115	differential extractability	1089:1115	differential extractability of a range of heterogeneous oligosaccharide epitopes	1089:1168	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	3	62	theme	greater	653:659	arg1	some					696:699	some	696:699	some	696:699	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	3	62	theme	greater	653:659	arg1	number					661:666	a far greater number	647:666	a far greater number	647:666	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	5	63	theme	differential	1089:1100	arg1	extractability					1102:1115	differential extractability	1089:1115	differential extractability of a range of heterogeneous oligosaccharide epitopes	1089:1168	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	5	64	theme	profiling	976:984	arg1	fractionation					987:999	Glycome profiling, fractionation and enzymatic digestion studies	968:1031	fractionation	987:999	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	6	65	theme	stress	1435:1440	arg1	responses					1442:1450	biotic stress responses	1428:1450	biotic stress responses	1428:1450	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	6	66	theme	matrix	1316:1321	arg1	polysaccharides					1323:1337	plant cell wall matrix polysaccharides	1300:1337	plant cell wall matrix polysaccharides	1300:1337	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	5	67	theme	lignin	1062:1067	arg1	compositions					1069:1080	the different lignin compositions	1048:1080	the different lignin compositions	1048:1080	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	1	68	theme	transgenic	118:127	arg1	plants					129:134	transgenic plants	118:134	transgenic plants	118:134	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	1	69	theme	altered	207:213	arg1	composition					222:232	altered lignin composition	207:232	altered lignin composition	207:232	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	0	70	theme	lignin	60:65	arg1	compositions					67:78	altered lignin compositions	52:78	altered lignin compositions	52:78	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	5	71	theme	heterogeneous	1131:1143	arg1	epitopes					1161:1168	heterogeneous oligosaccharide epitopes	1131:1168	heterogeneous oligosaccharide epitopes	1131:1168	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	4	72	theme	defense	856:862	arg1	genes					864:868	the defense genes	852:868	the defense genes	852:868	Several of the defense genes were differentially induced by water-soluble extracts from cell walls of plants of the two lines.					
29949660	0	73	with	plants	40:45	arg1	compositions					67:78	altered lignin compositions	52:78	altered lignin compositions	52:78	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	3	74	theme	bacterial	546:554	arg1	attack					565:570	oomycete and bacterial pathogen attack	533:570	oomycete and bacterial pathogen attack	533:570	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	1	75	from	reduction	83:91	arg1	content					107:113	the lignin content	96:113	the lignin content in transgenic plants	96:134	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	1	76	theme	lignin	215:220	arg1	composition					222:232	altered lignin composition	207:232	altered lignin composition	207:232	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
29949660	0	77	theme	altered	52:58	arg1	compositions					67:78	altered lignin compositions	52:78	altered lignin compositions	52:78	Elicitors and defense gene induction in plants with altered lignin compositions.					
29949660	5	78	theme	different	1211:1219	arg1	polymers					1231:1238	different cell wall polymers	1211:1238	different cell wall polymers	1211:1238	Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.					
29949660	6	79	theme	plant	1300:1304	arg1	polysaccharides					1323:1337	plant cell wall matrix polysaccharides	1300:1337	plant cell wall matrix polysaccharides	1300:1337	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	3	80	theme	pathogen	556:563	arg1	attack					565:570	oomycete and bacterial pathogen attack	533:570	oomycete and bacterial pathogen attack	533:570	Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis-jasmone-mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.					
29949660	6	81	theme	signal	1393:1398	arg1	molecules					1400:1408	multiple latent defense signal molecules	1369:1408	multiple latent defense signal molecules	1369:1408	Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.					
29949660	1	82	theme	composition	222:232	arg1	importance					193:202	the importance	189:202	the importance of altered lignin composition in such phenomena	189:250	A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.					
30583384	3	0	theme	cell	314:317	arg1	composition					324:334	berry skin cell wall composition	303:334	berry skin cell wall composition	303:334	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	5	1	theme	wall	749:752	arg1	compositions					754:765	the grape cell wall compositions	734:765	the grape cell wall compositions	734:765	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	1	2	theme	Vintage	154:160	arg1	effects					175:181	Vintage and ripeness effects	154:181	Vintage and ripeness effects	154:181	Part I: Vintage and ripeness effects.					
30583384	7	3	from	2015	994:997	arg1	intact					984:989	intact	984:989	intact	984:989	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	3	4	theme	wall	319:322	arg1	composition					324:334	berry skin cell wall composition	303:334	berry skin cell wall composition	303:334	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	6	5	theme	wine	796:799	arg1	content					810:816	a higher grape and wine phenolic content	777:816	a higher grape and wine phenolic content	777:816	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	0	6	theme	phenolic	108:115	arg1	compounds					117:125	phenolic compounds	108:125	phenolic compounds	108:125	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	8	7	theme	corresponding	1140:1152	arg1	extractability					1163:1176	the corresponding phenolic extractability	1136:1176	the corresponding phenolic extractability during the winemaking	1136:1198	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	4	8	theme	cell	567:570	arg1	walls					572:576	the grape cell walls	557:576	the grape cell walls	557:576	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	3	9	theme	grape	360:364	arg1	phenolics					375:383	grape and wine phenolics	360:383	grape and wine phenolics	360:383	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	5	10	theme	climatic	646:653	arg1	variance					655:662	The climatic variance	642:662	The climatic variance between the seasons	642:682	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	6	11	theme	grape	786:790	arg1	content					810:816	a higher grape and wine phenolic content	777:816	a higher grape and wine phenolic content	777:816	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	4	12	theme	grape	561:565	arg1	walls					572:576	the grape cell walls	557:576	the grape cell walls	557:576	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	6	13	theme	higher	779:784	arg1	content					810:816	a higher grape and wine phenolic content	777:816	a higher grape and wine phenolic content	777:816	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	2	14	theme	wine	276:279	arg1	stability					236:244	colour stability	229:244	colour stability	229:244	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	2	14	theme	wine	276:279	arg1	properties					258:267	sensory properties	250:267	sensory properties of red wine	250:279	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	1	15	theme	ripeness	166:173	arg1	effects					175:181	Vintage and ripeness effects	154:181	Vintage and ripeness effects	154:181	Part I: Vintage and ripeness effects.					
30583384	0	16	theme	compounds	117:125	arg1	composition					70:80	grape cell wall polysaccharide composition	39:80	grape cell wall polysaccharide composition	39:80	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	0	16	theme	compounds	117:125	arg1	extractability					90:103	the extractability	86:103	the extractability of phenolic compounds into Shiraz wines	86:143	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	6	17	from	content	810:816	arg1	phenols					843:849	polymeric phenols	833:849	polymeric phenols	833:849	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	7	18	theme	cell	903:906	arg1	walls					908:912	grape berry cell walls	891:912	grape berry cell walls	891:912	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	2	19	theme	red	272:274	arg1	wine					276:279	red wine	272:279	red wine	272:279	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	7	20	from	2016	1007:1010	arg1	intact					984:989	intact	984:989	intact	984:989	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	7	21	from	stages	942:947	arg1	walls					908:912	grape berry cell walls	891:912	grape berry cell walls	891:912	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	4	22	from	fraction	545:552	arg1	effect					489:494	The vintage effect	477:494	The vintage effect	477:494	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	4	22	from	fraction	545:552	arg1	significant					507:517	significant	507:517	significant	507:517	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	3	23	dep	levels	407:412	arg1	23°Brix					424:430	23°Brix	424:430	23°Brix	424:430	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	23	dep	levels	407:412	arg1	25°Brix					437:443	25°Brix	437:443	25°Brix	437:443	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	23	dep	levels	407:412	arg1	21°Brix					415:421	21°Brix	415:421	21°Brix	415:421	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	23	dep	levels	407:412	arg1	levels					407:412	different ripeness levels	388:412	different ripeness levels (21°Brix, 23°Brix, and 25°Brix)	388:444	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	8	24	theme	possible	1021:1028	arg1	relationship					1030:1041	a possible relationship	1019:1041	a possible relationship	1019:1041	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	7	25	theme	grape	891:895	arg1	walls					908:912	grape berry cell walls	891:912	grape berry cell walls	891:912	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	0	26	theme	wall	50:53	arg1	composition					70:80	grape cell wall polysaccharide composition	39:80	grape cell wall polysaccharide composition	39:80	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	6	27	located	found	856:860	arg2	content					810:816	a higher grape and wine phenolic content	777:816	a higher grape and wine phenolic content	777:816	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	6	27	located	found	856:860	arg1	wines					870:874	2015 wines	865:874	2015 wines	865:874	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	7	28	theme	berry	897:901	arg1	walls					908:912	grape berry cell walls	891:912	grape berry cell walls	891:912	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	4	29	from	significant	507:517	arg1	fraction					545:552	the pectin fraction	534:552	the pectin fraction of the grape cell walls	534:576	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	3	30	theme	wine	370:373	arg1	phenolics					375:383	grape and wine phenolics	360:383	grape and wine phenolics	360:383	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	0	31	theme	Shiraz	132:137	arg1	wines					139:143	Shiraz wines	132:143	Shiraz wines	132:143	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	2	32	theme	Phenolic	184:191	arg1	compounds					193:201	Phenolic compounds	184:201	Phenolic compounds	184:201	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	0	33	theme	cell	45:48	arg1	composition					70:80	grape cell wall polysaccharide composition	39:80	grape cell wall polysaccharide composition	39:80	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	4	34	theme	pectin	538:543	arg1	fraction					545:552	the pectin fraction	534:552	the pectin fraction of the grape cell walls	534:576	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	4	35	theme	phenolics	621:629	arg1	concentrations					595:608	the concentrations	591:608	the concentrations of certain phenolics extracted	591:639	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	0	36	theme	grape	39:43	arg1	composition					70:80	grape cell wall polysaccharide composition	39:80	grape cell wall polysaccharide composition	39:80	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	6	37	theme	polymeric	833:841	arg1	phenols					843:849	polymeric phenols	833:849	polymeric phenols	833:849	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	2	38	theme	colour	229:234	arg1	stability					236:244	colour stability	229:244	colour stability	229:244	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	4	39	theme	certain	613:619	arg1	phenolics					621:629	certain phenolics	613:629	certain phenolics extracted	613:639	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	0	40	theme	polysaccharide	55:68	arg1	composition					70:80	grape cell wall polysaccharide composition	39:80	grape cell wall polysaccharide composition	39:80	Investigating the relationship between grape cell wall polysaccharide composition and the extractability of phenolic compounds into Shiraz wines.					
30583384	8	41	theme	intactness	1081:1090	arg1	degree					1065:1070	the degree	1061:1070	the degree of berry intactness, especially for pectin-rich components,	1061:1130	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	8	41	theme	intactness	1081:1090	arg1	extractability					1163:1176	the corresponding phenolic extractability	1136:1176	the corresponding phenolic extractability during the winemaking	1136:1198	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	3	42	theme	berry	303:307	arg1	composition					324:334	berry skin cell wall composition	303:334	berry skin cell wall composition	303:334	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	5	43	theme	cell	744:747	arg1	compositions					754:765	the grape cell wall compositions	734:765	the grape cell wall compositions	734:765	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	3	44	theme	ripeness	398:405	arg1	23°Brix					424:430	23°Brix	424:430	23°Brix	424:430	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	44	theme	ripeness	398:405	arg1	25°Brix					437:443	25°Brix	437:443	25°Brix	437:443	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	44	theme	ripeness	398:405	arg1	21°Brix					415:421	21°Brix	415:421	21°Brix	415:421	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	44	theme	ripeness	398:405	arg1	levels					407:412	different ripeness levels	388:412	different ripeness levels (21°Brix, 23°Brix, and 25°Brix)	388:444	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	45	theme	skin	309:312	arg1	composition					324:334	berry skin cell wall composition	303:334	berry skin cell wall composition	303:334	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	2	46	theme	important	211:219	arg1	role					221:224	an important role	208:224	an important role	208:224	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	1	47	dep	effects	175:181	arg1	Part					146:149	Part I	146:151	Part I	146:151	Part I: Vintage and ripeness effects.					
30583384	8	48	theme	berry	1075:1079	arg1	intactness					1081:1090	berry intactness	1075:1090	berry intactness	1075:1090	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	5	49	located	observed	722:729	arg2	differences					710:720	the differences	706:720	the differences observed in the grape cell wall compositions	706:765	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	5	49	located	observed	722:729	arg1	compositions					754:765	the grape cell wall compositions	734:765	the grape cell wall compositions	734:765	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	3	50	theme	consecutive	455:465	arg1	vintages					467:474	two consecutive vintages	451:474	two consecutive vintages	451:474	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	8	51	theme	phenolic	1154:1161	arg1	extractability					1163:1176	the corresponding phenolic extractability	1136:1176	the corresponding phenolic extractability during the winemaking	1136:1198	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	4	52	theme	walls	572:576	arg1	fraction					545:552	the pectin fraction	534:552	the pectin fraction of the grape cell walls	534:576	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	5	53	theme	grape	738:742	arg1	compositions					754:765	the grape cell wall compositions	734:765	the grape cell wall compositions	734:765	The climatic variance between the seasons might have influenced the differences observed in the grape cell wall compositions.					
30583384	6	54	theme	phenolic	801:808	arg1	content					810:816	a higher grape and wine phenolic content	777:816	a higher grape and wine phenolic content	777:816	Firstly, a higher grape and wine phenolic content, especially in polymeric phenols, was found in 2015 wines.					
30583384	2	55	theme	sensory	250:256	arg1	properties					258:267	sensory properties	250:267	sensory properties of red wine	250:279	Phenolic compounds play an important role in colour stability and sensory properties of red wine.					
30583384	7	56	theme	ripening	952:959	arg1	stages					942:947	the earliest stages	929:947	the earliest stages of ripening	929:959	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	7	57	from	intact	984:989	arg1	2015					994:997	2015	994:997	2015	994:997	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	7	57	from	intact	984:989	arg1	2016					1007:1010	2016	1007:1010	2016	1007:1010	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	3	58	theme	different	388:396	arg1	23°Brix					424:430	23°Brix	424:430	23°Brix	424:430	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	58	theme	different	388:396	arg1	25°Brix					437:443	25°Brix	437:443	25°Brix	437:443	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	58	theme	different	388:396	arg1	21°Brix					415:421	21°Brix	415:421	21°Brix	415:421	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	3	58	theme	different	388:396	arg1	levels					407:412	different ripeness levels	388:412	different ripeness levels (21°Brix, 23°Brix, and 25°Brix)	388:444	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
30583384	4	59	theme	vintage	481:487	arg1	effect					489:494	The vintage effect	477:494	The vintage effect	477:494	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	4	59	theme	vintage	481:487	arg1	significant					507:517	significant	507:517	significant	507:517	The vintage effect was highly significant, especially in the pectin fraction of the grape cell walls and affected the concentrations of certain phenolics extracted.					
30583384	7	60	theme	earliest	933:940	arg1	stages					942:947	the earliest stages	929:947	the earliest stages of ripening	929:959	Additionally, grape berry cell walls, especially at the earliest stages of ripening, were found to be more intact in 2015 than in 2016.					
30583384	8	61	theme	pectin-rich	1108:1118	arg1	components					1120:1129	pectin-rich components	1108:1129	pectin-rich components	1108:1129	Thus, a possible relationship was found between the degree of berry intactness, especially for pectin-rich components, and the corresponding phenolic extractability during the winemaking.					
30583384	3	62	dep	evaluated	293:301	arg1	influences					349:358	influences	349:358	influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages	349:474	This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages.					
31336117	7	0	theme	Hep3B	1301:1305	arg1	cells					1307:1311	Hep3B cells	1301:1311	Hep3B cells	1301:1311	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	0	1	from	screening	21:29	arg1	hijiki					86:91	acid-processed hijiki	71:91	acid-processed hijiki (Hizikia fusiforme)	71:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	1	from	screening	21:29	arg1	fusiforme					102:110	Hizikia fusiforme	94:110	Hizikia fusiforme	94:110	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	5	2	theme	half-maximal	854:865	arg1	concentration					878:890	a half-maximal inhibitory concentration	852:890	a half-maximal inhibitory concentration	852:890	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	4	3	theme	Fourier-transform	714:730	arg1	spectroscopy					741:752	Fourier-transform infrared spectroscopy	714:752	Fourier-transform infrared spectroscopy	714:752	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	1	4	theme	East	306:309	arg1	Asia					311:314	East Asia	306:314	East Asia	306:314	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	7	5	theme	Bcl-2-associated	1217:1232	arg1	protein					1236:1242	Bcl-2-associated X protein	1217:1242	Bcl-2-associated X protein	1217:1242	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	0	6	from	Characterization	0:15	arg1	hijiki					86:91	acid-processed hijiki	71:91	acid-processed hijiki (Hizikia fusiforme)	71:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	6	from	Characterization	0:15	arg1	fusiforme					102:110	Hizikia fusiforme	94:110	Hizikia fusiforme	94:110	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	3	7	theme	active	497:502	arg1	JHCF1-JHCF4					515:525	JHCF1-JHCF4	515:525	JHCF1-JHCF4	515:525	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	3	7	theme	active	497:502	arg1	fractions					504:512	four active fractions	492:512	four active fractions (JHCF1-JHCF4)	492:526	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	8	8	theme	potential	1441:1449	arg1	loss					1410:1413	the concurrent loss	1395:1413	the concurrent loss of mitochondrial membrane potential	1395:1449	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	5	9	with	48 h	842:845	arg1	concentration					878:890	a half-maximal inhibitory concentration	852:890	a half-maximal inhibitory concentration	852:890	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	0	10	theme	Hizikia	94:100	arg1	hijiki					86:91	acid-processed hijiki	71:91	acid-processed hijiki (Hizikia fusiforme)	71:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	10	theme	Hizikia	94:100	arg1	fusiforme					102:110	Hizikia fusiforme	94:110	Hizikia fusiforme	94:110	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	8	11	theme	membrane	1432:1439	arg1	potential					1441:1449	mitochondrial membrane potential	1418:1449	mitochondrial membrane potential	1418:1449	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	5	12	theme	anticancer	947:956	arg1	activity					958:965	the strongest anticancer activity	933:965	the strongest anticancer activity	933:965	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	9	13	theme	antiproliferative	1575:1591	arg1	effects					1593:1599	antiproliferative effects	1575:1599	antiproliferative effects	1575:1599	Therefore, these results indicate that JHCF4 exhibited antiproliferative effects on the investigated cancer cell lines.					
31336117	5	14	theme	33.53 ± 2.50 μg/ml	895:912	arg1	concentration					878:890	a half-maximal inhibitory concentration	852:890	a half-maximal inhibitory concentration	852:890	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	5	15	theme	highest	772:778	arg1	fucose					780:785	fucose	780:785	fucose	780:785	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	9	16	theme	investigated	1608:1619	arg1	lines					1633:1637	the investigated cancer cell lines	1604:1637	the investigated cancer cell lines	1604:1637	Therefore, these results indicate that JHCF4 exhibited antiproliferative effects on the investigated cancer cell lines.					
31336117	7	17	theme	lymphoma	1175:1182	arg1	extra-large					1184:1194	B-cell lymphoma extra-large	1168:1194	B-cell lymphoma extra-large	1168:1194	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	5	18	theme	inhibitory	867:876	arg1	concentration					878:890	a half-maximal inhibitory concentration	852:890	a half-maximal inhibitory concentration	852:890	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	6	19	theme	JHCF4	1104:1108	arg1	activity					1092:1099	the anticancer activity	1077:1099	the anticancer activity of JHCF4	1077:1108	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	4	20	theme	fractions	685:693	arg1	structure					628:636	The structure	624:636	The structure of the crude polysaccharides and four fucoidan fractions	624:693	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	5	21	theme	Hep3B	821:825	arg1	growth					832:837	Hep3B cell growth	821:837	Hep3B cell growth	821:837	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	7	22	theme	X	1234:1234	arg1	protein					1236:1242	Bcl-2-associated X protein	1217:1242	Bcl-2-associated X protein	1217:1242	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	9	23	theme	cancer	1621:1626	arg1	lines					1633:1637	the investigated cancer cell lines	1604:1637	the investigated cancer cell lines	1604:1637	Therefore, these results indicate that JHCF4 exhibited antiproliferative effects on the investigated cancer cell lines.					
31336117	1	24	theme	antiproliferative	159:175	arg1	effects					177:183	the antiproliferative effects	155:183	the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia	155:314	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	7	25	theme	apoptotic	1260:1268	arg1	bodies					1270:1275	apoptotic bodies	1260:1275	apoptotic bodies	1260:1275	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	4	26	theme	crude	645:649	arg1	polysaccharides					651:665	the crude polysaccharides	641:665	the crude polysaccharides	641:665	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	8	27	theme	potential	1467:1475	arg1	role					1477:1480	the potential role	1463:1480	the potential role of the mitochondria-mediated pathway	1463:1517	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	2	28	theme	%	345:345	arg1	acid					354:357	1% citric acid	344:357	1% citric acid	344:357	Hijiki was processed using 1% citric acid to decrease heavy metal content.					
31336117	6	29	theme	bromide	1042:1048	arg1	staining					1050:1057	acridine orange-ethidium bromide staining	1017:1057	acridine orange-ethidium bromide staining	1017:1057	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	7	30	theme	B-cell	1168:1173	arg1	lymphoma					1175:1182	B-cell lymphoma	1168:1182	B-cell lymphoma extra-large	1168:1194	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	6	31	with	staining	985:992	arg1	Hoechst					999:1005	Hoechst 33342	999:1011	Hoechst 33342	999:1011	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	2	32	theme	1	344:344	arg1	%					345:345	%	345:345	%	345:345	Hijiki was processed using 1% citric acid to decrease heavy metal content.					
31336117	6	33	theme	orange-ethidium	1026:1040	arg1	staining					1050:1057	acridine orange-ethidium bromide staining	1017:1057	acridine orange-ethidium bromide staining	1017:1057	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	8	34	theme	mitochondria-mediated	1489:1509	arg1	pathway					1511:1517	the mitochondria-mediated pathway	1485:1517	the mitochondria-mediated pathway	1485:1517	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	2	35	theme	metal	377:381	arg1	content					383:389	heavy metal content	371:389	heavy metal content	371:389	Hijiki was processed using 1% citric acid to decrease heavy metal content.					
31336117	6	36	theme	acridine	1017:1024	arg1	staining					1050:1057	acridine orange-ethidium bromide staining	1017:1057	acridine orange-ethidium bromide staining	1017:1057	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	9	37	theme	cell	1628:1631	arg1	lines					1633:1637	the investigated cancer cell lines	1604:1637	the investigated cancer cell lines	1604:1637	Therefore, these results indicate that JHCF4 exhibited antiproliferative effects on the investigated cancer cell lines.					
31336117	3	38	theme	monosaccharide	538:551	arg1	composition					553:563	their monosaccharide composition	532:563	their monosaccharide composition	532:563	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	2	39	theme	heavy	371:375	arg1	content					383:389	heavy metal content	371:389	heavy metal content	371:389	Hijiki was processed using 1% citric acid to decrease heavy metal content.					
31336117	0	40	theme	activity	45:52	arg1	screening					21:29	screening	21:29	screening	21:29	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	40	theme	activity	45:52	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	8	41	theme	species	1376:1382	arg1	generation					1346:1355	the generation	1342:1355	the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential	1342:1449	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	0	42	from	hijiki	86:91	arg1	screening					21:29	screening	21:29	screening	21:29	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	42	from	hijiki	86:91	arg1	activity					45:52	anti-tumor activity	34:52	anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme)	34:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	42	from	hijiki	86:91	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	3	43	theme	high-performance	584:599	arg1	chromatography					608:621	high-performance liquid chromatography	584:621	high-performance liquid chromatography	584:621	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	4	44	theme	fucoidan	676:683	arg1	fractions					685:693	four fucoidan fractions	671:693	four fucoidan fractions	671:693	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	4	45	theme	polysaccharides	651:665	arg1	structure					628:636	The structure	624:636	The structure of the crude polysaccharides and four fucoidan fractions	624:693	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	0	46	theme	anti-tumor	34:43	arg1	activity					45:52	anti-tumor activity	34:52	anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme)	34:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	1	47	from	samples	244:250	arg1	effects					177:183	the antiproliferative effects	155:183	the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia	155:314	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	47	from	samples	244:250	arg1	fucoidan					188:195	fucoidan	188:195	fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	188:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	7	48	from	degrees	1290:1296	arg1	cells					1307:1311	Hep3B cells	1301:1311	Hep3B cells	1301:1311	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	3	49	theme	liquid	601:606	arg1	chromatography					608:621	high-performance liquid chromatography	584:621	high-performance liquid chromatography	584:621	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	1	50	theme	regional	208:215	arg1	samples					244:250	three regional hijiki (Hizikia fusiforme) samples	202:250	three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	202:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	50	theme	regional	208:215	arg1	Wando-Korea					290:300	Wando-Korea	290:300	Wando-Korea	290:300	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	50	theme	regional	208:215	arg1	Jeju-Korea					269:278	Jeju-Korea	269:278	Jeju-Korea	269:278	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	50	theme	regional	208:215	arg1	Zhejiang-China					253:266	Zhejiang-China	253:266	Zhejiang-China	253:266	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	0	51	theme	fucoidan	57:64	arg1	activity					45:52	anti-tumor activity	34:52	anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme)	34:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	5	52	theme	sulfate	791:797	arg1	content					799:805	sulfate content	791:805	sulfate content	791:805	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	8	53	theme	concurrent	1399:1408	arg1	loss					1410:1413	the concurrent loss	1395:1413	the concurrent loss of mitochondrial membrane potential	1395:1449	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	7	54	theme	different	1280:1288	arg1	degrees					1290:1296	different degrees	1280:1296	different degrees in Hep3B cells	1280:1311	Moreover, JHCF4 down-regulated B-cell lymphoma extra-large, while up-regulating Bcl-2-associated X protein, caspase-3, and apoptotic bodies to different degrees in Hep3B cells.					
31336117	8	55	theme	pathway	1511:1517	arg1	role					1477:1480	the potential role	1463:1480	the potential role of the mitochondria-mediated pathway	1463:1517	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	4	56	theme	infrared	732:739	arg1	spectroscopy					741:752	Fourier-transform infrared spectroscopy	714:752	Fourier-transform infrared spectroscopy	714:752	The structure of the crude polysaccharides and four fucoidan fractions was analyzed using Fourier-transform infrared spectroscopy.					
31336117	8	57	theme	oxygen	1369:1374	arg1	species					1376:1382	reactive oxygen species	1360:1382	reactive oxygen species	1360:1382	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	3	58	theme	diethylaminoethyl-cellulose-ion	426:456	arg1	chromatography					467:480	diethylaminoethyl-cellulose-ion exchange chromatography	426:480	diethylaminoethyl-cellulose-ion exchange chromatography	426:480	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	1	59	theme	hijiki	217:222	arg1	samples					244:250	three regional hijiki (Hizikia fusiforme) samples	202:250	three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	202:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	59	theme	hijiki	217:222	arg1	Wando-Korea					290:300	Wando-Korea	290:300	Wando-Korea	290:300	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	59	theme	hijiki	217:222	arg1	Jeju-Korea					269:278	Jeju-Korea	269:278	Jeju-Korea	269:278	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	59	theme	hijiki	217:222	arg1	Zhejiang-China					253:266	Zhejiang-China	253:266	Zhejiang-China	253:266	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	6	60	with	staining	1050:1057	arg1	Hoechst					999:1005	Hoechst 33342	999:1011	Hoechst 33342	999:1011	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	1	61	from	effects	177:183	arg1	Asia					311:314	East Asia	306:314	East Asia	306:314	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	61	from	effects	177:183	arg1	samples					244:250	three regional hijiki (Hizikia fusiforme) samples	202:250	three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	202:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	61	from	effects	177:183	arg1	Wando-Korea					290:300	Wando-Korea	290:300	Wando-Korea	290:300	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	61	from	effects	177:183	arg1	Jeju-Korea					269:278	Jeju-Korea	269:278	Jeju-Korea	269:278	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	61	from	effects	177:183	arg1	Zhejiang-China					253:266	Zhejiang-China	253:266	Zhejiang-China	253:266	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	3	62	theme	exchange	458:465	arg1	chromatography					467:480	diethylaminoethyl-cellulose-ion exchange chromatography	426:480	diethylaminoethyl-cellulose-ion exchange chromatography	426:480	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	8	63	theme	mitochondrial	1418:1430	arg1	potential					1441:1449	mitochondrial membrane potential	1418:1449	mitochondrial membrane potential	1418:1449	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	3	64	theme	JH	396:397	arg1	sample					399:404	The JH sample	392:404	The JH sample	392:404	The JH sample was separated using diethylaminoethyl-cellulose-ion exchange chromatography to obtain four active fractions (JHCF1-JHCF4) and their monosaccharide composition was detected using high-performance liquid chromatography.					
31336117	0	65	theme	acid-processed	71:84	arg1	hijiki					86:91	acid-processed hijiki	71:91	acid-processed hijiki (Hizikia fusiforme)	71:111	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	0	65	theme	acid-processed	71:84	arg1	fusiforme					102:110	Hizikia fusiforme	94:110	Hizikia fusiforme	94:110	Characterization and screening of anti-tumor activity of fucoidan from acid-processed hijiki (Hizikia fusiforme).					
31336117	1	66	dep	samples	244:250	arg1	samples					244:250	three regional hijiki (Hizikia fusiforme) samples	202:250	three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	202:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	66	dep	samples	244:250	arg1	Wando-Korea					290:300	Wando-Korea	290:300	Wando-Korea	290:300	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	66	dep	samples	244:250	arg1	Jeju-Korea					269:278	Jeju-Korea	269:278	Jeju-Korea	269:278	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	66	dep	samples	244:250	arg1	Zhejiang-China					253:266	Zhejiang-China	253:266	Zhejiang-China	253:266	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	8	67	theme	reactive	1360:1367	arg1	species					1376:1382	reactive oxygen species	1360:1382	reactive oxygen species	1360:1382	JHCF4 induced apoptosis via the generation of reactive oxygen species along with the concurrent loss of mitochondrial membrane potential, indicating the potential role of the mitochondria-mediated pathway.					
31336117	1	68	theme	fucoidan	188:195	arg1	effects					177:183	the antiproliferative effects	155:183	the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia	155:314	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	69	theme	study	130:134	arg1	aim					118:120	The aim	114:120	The aim of this study	114:134	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	2	70	theme	citric	347:352	arg1	acid					354:357	1% citric acid	344:357	1% citric acid	344:357	Hijiki was processed using 1% citric acid to decrease heavy metal content.					
31336117	1	71	theme	Hizikia	225:231	arg1	hijiki					217:222	hijiki	217:222	three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea)	202:301	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	1	71	theme	Hizikia	225:231	arg1	fusiforme					233:241	Hizikia fusiforme	225:241	Hizikia fusiforme	225:241	The aim of this study was to investigate the antiproliferative effects of fucoidan from three regional hijiki (Hizikia fusiforme) samples (Zhejiang-China, Jeju-Korea [JH], and Wando-Korea) in East Asia.					
31336117	5	72	theme	cell	827:830	arg1	growth					832:837	Hep3B cell growth	821:837	Hep3B cell growth	821:837	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	6	73	theme	nuclear	977:983	arg1	staining					985:992	nuclear staining	977:992	nuclear staining with Hoechst 33342	977:1011	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
31336117	5	74	theme	strongest	937:945	arg1	activity					958:965	the strongest anticancer activity	933:965	the strongest anticancer activity	933:965	JHCF4 showed the highest fucose and sulfate content and decreased Hep3B cell growth in 48 h with a half-maximal inhibitory concentration of 33.53 ± 2.50 μg/ml, which represented the strongest anticancer activity.					
31336117	6	75	theme	anticancer	1081:1090	arg1	activity					1092:1099	the anticancer activity	1077:1099	the anticancer activity of JHCF4	1077:1108	Further, nuclear staining with Hoechst 33342 and acridine orange-ethidium bromide staining demonstrated that the anticancer activity of JHCF4 was mediated by apoptosis.					
29663128	0	0	theme	Lanzhou	83:89	arg1	Lily					91:94	Lanzhou Lily	83:94	Lanzhou Lily	83:94	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	3	1	theme	methylation	288:298	arg1	analysis					300:307	methylation analysis	288:307	methylation analysis	288:307	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	4	2	theme	6.74	810:813	arg1	ratio					801:805	an approximate weight ratio	779:805	an approximate weight ratio of 6.74	779:813	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	3	3	theme	chromatography-mass	480:498	arg1	spectroscopy					500:511	gas chromatography-mass spectroscopy	476:511	gas chromatography-mass spectroscopy (GC-MS)	476:519	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	3	theme	chromatography-mass	480:498	arg1	GC-MS					514:518	GC-MS	514:518	GC-MS	514:518	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	1	4	theme	Lilium	217:222	arg1	var					232:234	Lilium davidii var	217:234	Lilium davidii var	217:234	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	3	5	theme	infrared	522:529	arg1	spectroscopy					536:547	infrared (IR) spectroscopy	522:547	infrared (IR) spectroscopy	522:547	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	5	6	theme	thermal	866:872	arg1	behavior					874:881	thermal behavior	866:881	thermal behavior	866:881	Furthermore, its morphology, thermal behavior, and preliminary hypoglycemic activities were also investigated.					
29663128	0	7	theme	Lily	91:94	arg1	Bulbs					74:78	Bulbs	74:78	Bulbs of Lanzhou Lily	74:94	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	4	8	theme	molecular	675:683	arg1	6.2 × 104 Da					692:703	molecular weight 6.2 × 104 Da	675:703	molecular weight 6.2 × 104 Da	675:703	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	8	theme	molecular	675:683	arg1	heteropolysaccharide					653:672	a homogeneous heteropolysaccharide	639:672	a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da)	639:704	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	3	9	theme	structural	314:323	arg1	features					325:332	its structural features	310:332	its structural features	310:332	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	4	10	theme	approximate	782:792	arg1	ratio					801:805	an approximate weight ratio	779:805	an approximate weight ratio of 6.74	779:813	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	11	dep	heteropolysaccharide	653:672	arg1	Glc					763:765	Glc	763:765	Glc	763:765	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	11	dep	heteropolysaccharide	653:672	arg1	Gal					772:774	Gal	772:774	Gal	772:774	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	11	dep	heteropolysaccharide	653:672	arg1	Man					758:760	Man	758:760	Man	758:760	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	11	dep	heteropolysaccharide	653:672	arg1	Lyx					753:755	Lyx	753:755	Lyx	753:755	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	5	12	theme	preliminary	888:898	arg1	activities					913:922	preliminary hypoglycemic activities	888:922	preliminary hypoglycemic activities	888:922	Furthermore, its morphology, thermal behavior, and preliminary hypoglycemic activities were also investigated.					
29663128	3	13	theme	size-exclusion	398:411	arg1	chromatography					413:426	size-exclusion chromatography	398:426	size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy	398:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	4	14	theme	weight	794:799	arg1	ratio					801:805	an approximate weight ratio	779:805	an approximate weight ratio of 6.74	779:813	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	3	15	theme	IR	532:533	arg1	spectroscopy					536:547	infrared (IR) spectroscopy	522:547	infrared (IR) spectroscopy	522:547	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	0	16	theme	Structural	14:23	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	1	17	dep	Lily	211:214	arg1	var					232:234	Lilium davidii var	217:234	Lilium davidii var	217:234	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	1	18	dep	polysaccharide	135:148	arg1	designated					151:160	designated	151:160	designated as LDP-2	151:169	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	4	19	dep	Lyx	753:755	arg1	10.48					829:833	10.48	829:833	10.48	829:833	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	19	dep	Lyx	753:755	arg1	76.50					822:826	76.50	822:826	76.50	822:826	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	19	dep	Lyx	753:755	arg1	6.28					816:819	6.28	816:819	6.28	816:819	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	20	theme	weight	685:690	arg1	6.2 × 104 Da					692:703	molecular weight 6.2 × 104 Da	675:703	molecular weight 6.2 × 104 Da	675:703	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	20	theme	weight	685:690	arg1	heteropolysaccharide					653:672	a homogeneous heteropolysaccharide	639:672	a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da)	639:704	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	3	21	theme	magnetic	562:569	arg1	spectroscopy					587:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	22	theme	laser	442:446	arg1	SEC-LLS					466:472	SEC-LLS	466:472	SEC-LLS	466:472	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	22	theme	laser	442:446	arg1	scattering					454:463	laser light scattering	442:463	laser light scattering (SEC-LLS)	442:473	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	0	23	from	Bulbs	74:78	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	0	23	from	Bulbs	74:78	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	3	24	theme	resonance	571:579	arg1	spectroscopy					587:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	25	theme	light	448:452	arg1	SEC-LLS					466:472	SEC-LLS	466:472	SEC-LLS	466:472	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	25	theme	light	448:452	arg1	scattering					454:463	laser light scattering	442:463	laser light scattering (SEC-LLS)	442:473	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	0	26	theme	Polysaccharide	54:67	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	0	26	theme	Polysaccharide	54:67	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	0	27	theme	Second	47:52	arg1	Polysaccharide					54:67	a Second Polysaccharide	45:67	a Second Polysaccharide	45:67	Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily.					
29663128	3	28	theme	nuclear	554:560	arg1	spectroscopy					587:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	29	theme	gas	476:478	arg1	spectroscopy					500:511	gas chromatography-mass spectroscopy	476:511	gas chromatography-mass spectroscopy (GC-MS)	476:519	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	29	theme	gas	476:478	arg1	GC-MS					514:518	GC-MS	514:518	GC-MS	514:518	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	1	30	attach	isolated	176:183	arg2	polysaccharide					135:148	a second water-soluble polysaccharide	112:148	a second water-soluble polysaccharide (designated as LDP-2)	112:170	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	1	30	attach	isolated	176:183	arg1	bulbs					194:198	the bulbs	190:198	the bulbs of Lanzhou Lily (Lilium davidii var	190:234	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	5	31	theme	hypoglycemic	900:911	arg1	activities					913:922	preliminary hypoglycemic activities	888:922	preliminary hypoglycemic activities	888:922	Furthermore, its morphology, thermal behavior, and preliminary hypoglycemic activities were also investigated.					
29663128	3	32	theme	monosaccharide	257:270	arg1	composition					272:282	monosaccharide composition	257:282	monosaccharide composition	257:282	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	1	33	dep	Lilium	217:222	arg1	davidii					224:230	davidii	224:230	davidii	224:230	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	4	34	theme	monosaccharides	736:750	arg1	kinds					727:731	four kinds	722:731	four kinds of monosaccharides	722:750	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	35	from	Glc	763:765	arg1	ratio					801:805	an approximate weight ratio	779:805	an approximate weight ratio of 6.74	779:813	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	1	36	theme	second	114:119	arg1	polysaccharide					135:148	a second water-soluble polysaccharide	112:148	a second water-soluble polysaccharide (designated as LDP-2)	112:170	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	1	37	theme	Lanzhou	203:209	arg1	Lily					211:214	Lanzhou Lily	203:214	Lanzhou Lily (Lilium davidii var	203:234	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	1	38	theme	water-soluble	121:133	arg1	polysaccharide					135:148	a second water-soluble polysaccharide	112:148	a second water-soluble polysaccharide (designated as LDP-2)	112:170	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	1	39	theme	Lily	211:214	arg1	bulbs					194:198	the bulbs	190:198	the bulbs of Lanzhou Lily (Lilium davidii var	190:234	In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var.					
29663128	3	40	theme	characterizations	371:387	arg1	variety					360:366	a variety	358:366	a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy	358:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	40	theme	characterizations	371:387	arg1	chromatography					413:426	size-exclusion chromatography	398:426	size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy	398:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	40	theme	characterizations	371:387	arg1	characterizations					371:387	characterizations	371:387	characterizations	371:387	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	3	41	theme	NMR	582:584	arg1	spectroscopy					587:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	nuclear magnetic resonance (NMR) spectroscopy	554:598	Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.					
29663128	4	42	from	Man	758:760	arg1	ratio					801:805	an approximate weight ratio	779:805	an approximate weight ratio of 6.74	779:813	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	43	theme	homogeneous	641:651	arg1	6.2 × 104 Da					692:703	molecular weight 6.2 × 104 Da	675:703	molecular weight 6.2 × 104 Da	675:703	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	43	theme	homogeneous	641:651	arg1	heteropolysaccharide					653:672	a homogeneous heteropolysaccharide	639:672	a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da)	639:704	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29663128	4	43	theme	homogeneous	641:651	arg1	LDP-2					630:634	LDP-2	630:634	LDP-2	630:634	The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48).					
29406618	7	0	theme	microcomputed	1202:1214	arg1	tomography					1216:1225	microcomputed tomography	1202:1225	microcomputed tomography	1202:1225	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	0	theme	microcomputed	1202:1214	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	1	theme	volume	1092:1097	arg1	measurement					1099:1109	volume measurement	1092:1109	volume measurement	1092:1109	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	6	2	theme	composite	869:877	arg1	parameters					879:888	the composite parameters	865:888	the composite parameters	865:888	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	8	3	theme	human	1295:1299	arg1	serum					1301:1305	human serum	1295:1305	human serum collected from healthy people	1295:1335	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	6	4	from	changes	912:918	arg1	reasonable					769:778	reasonable	769:778	reasonable	769:778	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	6	5	theme	volume	905:910	arg1	changes					912:918	particular volume changes	894:918	particular volume changes	894:918	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	5	6	theme	composite	640:648	arg1	implantation					650:661	the composite implantation	636:661	the composite implantation in patients without significant inflammation indicators	636:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	6	7	theme	pH	825:826	arg1	serum					808:812	human serum	802:812	human serum of neutral pH (typical for noninflamed tissues)	802:860	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	9	8	theme	change-related	1487:1500	arg1	effects					1507:1513	volume change-related side effects	1480:1513	volume change-related side effects of HAp/glucan composite	1480:1537	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	7	9	from	serum	1049:1053	arg1	parameters					986:995	physicochemical parameters	970:995	physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH	970:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	6	10	theme	particular	894:903	arg1	changes					912:918	particular volume changes	894:918	particular volume changes	894:918	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	5	11	theme	further	602:608	arg1	cases					619:623	further clinical cases	602:623	further clinical cases concerning the composite implantation in patients without significant inflammation indicators	602:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	2	12	theme	specific	278:285	arg1	conditions					292:301	the specific body conditions	274:301	the specific body conditions	274:301	However, such composites tested in vivo may behave different due to the specific body conditions.					
29406618	7	13	theme	parameters	986:995	arg1	characterization					950:965	the characterization	946:965	the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH	946:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	0	14	from	serum	57:61	arg1	composite					38:46	composite	38:46	composite	38:46	Behavior of new hydroxyapatite/glucan composite in human serum.					
29406618	5	15	theme	significant	683:693	arg1	indicators					708:717	significant inflammation indicators	683:717	significant inflammation indicators	683:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	5	16	theme	clinical	610:617	arg1	cases					619:623	further clinical cases	602:623	further clinical cases concerning the composite implantation in patients without significant inflammation indicators	602:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	7	17	theme	Fourier-transform	1161:1177	arg1	spectroscopy					1188:1199	Fourier-transform infrared spectroscopy	1161:1199	Fourier-transform infrared spectroscopy	1161:1199	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	17	theme	Fourier-transform	1161:1177	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	4	18	theme	massive	473:479	arg1	swelling					481:488	Such massive swelling	468:488	Such massive swelling in acidic medium	468:505	Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial.					
29406618	6	19	theme	serum	808:812	arg1	effect					792:797	the effect	788:797	the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters	788:888	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	7	20	theme	scanning	1112:1119	arg1	microscopy					1130:1139	scanning electron microscopy	1112:1139	scanning electron microscopy	1112:1139	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	20	theme	scanning	1112:1119	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	4	21	theme	acidic	493:498	arg1	medium					500:505	acidic medium	493:505	acidic medium	493:505	Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial.					
29406618	10	22	dep	©	1609:1609	arg1	Inc.					1635:1638	Inc.	1635:1638	Inc.	1635:1638	© 2018 Wiley Periodicals, Inc.					
29406618	6	23	theme	human	802:806	arg1	serum					808:812	human serum	802:812	human serum of neutral pH (typical for noninflamed tissues)	802:860	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	1	24	theme	bone	81:84	arg1	regeneration					93:104	bone tissue regeneration	81:104	bone tissue regeneration	81:104	Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials.					
29406618	5	25	located	observed	741:748	arg2	effects					728:734	no side effects	720:734	no side effects	720:734	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	5	25	located	observed	741:748	arg1	cases					619:623	further clinical cases	602:623	further clinical cases concerning the composite implantation in patients without significant inflammation indicators	602:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	7	26	from	parameters	986:995	arg1	serum					1049:1053	human serum	1043:1053	human serum of neutral pH	1043:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	0	27	from	composite	38:46	arg1	serum					57:61	human serum	51:61	human serum	51:61	Behavior of new hydroxyapatite/glucan composite in human serum.					
29406618	4	28	from	swelling	481:488	arg1	medium					500:505	acidic medium	493:505	acidic medium	493:505	Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial.					
29406618	1	29	theme	tissue	86:91	arg1	regeneration					93:104	bone tissue regeneration	81:104	bone tissue regeneration	81:104	Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials.					
29406618	6	30	from	reasonable	769:778	arg1	changes					912:918	particular volume changes	894:918	particular volume changes	894:918	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	3	31	theme	transient	376:384	arg1	inflammation					400:411	transient postoperative inflammation	376:411	transient postoperative inflammation	376:411	For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing.					
29406618	1	32	theme	clinical	189:196	arg1	trials					198:203	the clinical trials	185:203	the clinical trials	185:203	Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials.					
29406618	5	33	from	implantation	650:661	arg1	patients					666:673	patients	666:673	patients without significant inflammation indicators	666:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	5	34	theme	side	723:726	arg1	effects					728:734	no side effects	720:734	no side effects	720:734	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	8	35	theme	mechanical	1403:1412	arg1	changes					1360:1366	no uncontrolled changes	1344:1366	no uncontrolled changes in weight and volume	1344:1387	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	35	theme	mechanical	1403:1412	arg1	properties					1414:1423	mechanical properties	1403:1423	mechanical properties of the composite	1403:1440	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	6	36	from	effect	792:797	arg1	parameters					879:888	the composite parameters	865:888	the composite parameters	865:888	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	0	37	theme	new	12:14	arg1	Behavior					0:7	Behavior	0:7	Behavior of new	0:14	Behavior of new hydroxyapatite/glucan composite in human serum.					
29406618	4	38	theme	/β-glucan	567:575	arg1	biomaterial					577:587	new elastic hydroxyapatite (HAp)/β-glucan biomaterial	535:587	new elastic hydroxyapatite (HAp)/β-glucan biomaterial	535:587	Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial.					
29406618	7	39	theme	neutral	1058:1064	arg1	pH					1066:1067	neutral pH	1058:1067	neutral pH	1058:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	2	40	theme	body	287:290	arg1	conditions					292:301	the specific body conditions	274:301	the specific body conditions	274:301	However, such composites tested in vivo may behave different due to the specific body conditions.					
29406618	9	41	theme	HAp/glucan	1518:1527	arg1	composite					1529:1537	HAp/glucan composite	1518:1537	HAp/glucan composite	1518:1537	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	7	42	theme	electron	1121:1128	arg1	microscopy					1130:1139	scanning electron microscopy	1112:1139	scanning electron microscopy	1112:1139	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	42	theme	electron	1121:1128	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	43	theme	human	1043:1047	arg1	serum					1049:1053	human serum	1043:1053	human serum of neutral pH	1043:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	3	44	theme	wound	453:457	arg1	healing					459:465	the wound healing	449:465	the wound healing	449:465	For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing.					
29406618	4	45	theme	Such	468:471	arg1	swelling					481:488	Such massive swelling	468:488	Such massive swelling in acidic medium	468:505	Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial.					
29406618	7	46	theme	infrared	1179:1186	arg1	spectroscopy					1188:1199	Fourier-transform infrared spectroscopy	1161:1199	Fourier-transform infrared spectroscopy	1161:1199	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	46	theme	infrared	1179:1186	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	3	47	theme	postoperative	386:398	arg1	inflammation					400:411	transient postoperative inflammation	376:411	transient postoperative inflammation	376:411	For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing.					
29406618	5	48	theme	inflammation	695:706	arg1	indicators					708:717	significant inflammation indicators	683:717	significant inflammation indicators	683:717	However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed.					
29406618	7	49	theme	pH	1066:1067	arg1	serum					1049:1053	human serum	1043:1053	human serum of neutral pH	1043:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	11	50	dep	Res	1655:1657	arg1	106B					1682:1685	106B	1682:1685	106B	1682:1685	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	11	50	dep	Res	1655:1657	arg1	B					1664:1664	Part B	1659:1664	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.	1640:1703	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	11	50	dep	Res	1655:1657	arg1	Biomater					1672:1679	Biomater	1672:1679	Biomater	1672:1679	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	11	50	dep	Res	1655:1657	arg1	2018					1699:1702	2018	1699:1702	2018	1699:1702	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	1	51	theme	polymer-based	117:129	arg1	composites					131:140	polymer-based composites	117:140	polymer-based composites	117:140	Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials.					
29406618	0	52	theme	human	51:55	arg1	serum					57:61	human serum	51:61	human serum	51:61	Behavior of new hydroxyapatite/glucan composite in human serum.					
29406618	11	53	dep	Biomater	1672:1679	arg1	2653-2664					1688:1696	2653-2664	1688:1696	2653-2664	1688:1696	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	6	54	theme	noninflamed	841:851	arg1	tissues					853:859	noninflamed tissues	841:859	noninflamed tissues	841:859	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	2	55	theme	such	215:218	arg1	composites					220:229	such composites	215:229	such composites tested in vivo	215:244	However, such composites tested in vivo may behave different due to the specific body conditions.					
29406618	7	56	theme	composite	1004:1012	arg1	parameters					986:995	physicochemical parameters	970:995	physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH	970:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	9	57	theme	side	1502:1505	arg1	effects					1507:1513	volume change-related side effects	1480:1513	volume change-related side effects of HAp/glucan composite	1480:1537	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	8	58	theme	composite	1432:1440	arg1	changes					1360:1366	no uncontrolled changes	1344:1366	no uncontrolled changes in weight and volume	1344:1387	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	58	theme	composite	1432:1440	arg1	porosity					1390:1397	porosity	1390:1397	porosity	1390:1397	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	58	theme	composite	1432:1440	arg1	properties					1414:1423	mechanical properties	1403:1423	mechanical properties of the composite	1403:1440	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	6	59	theme	neutral	817:823	arg1	pH					825:826	neutral pH	817:826	neutral pH (typical for noninflamed tissues)	817:860	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	9	60	theme	effects	1507:1513	arg1	lack					1472:1475	the lack	1468:1475	the lack of volume change-related side effects of HAp/glucan composite in bone	1468:1545	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	8	61	from	changes	1360:1366	arg1	volume					1382:1387	volume	1382:1387	volume	1382:1387	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	61	from	changes	1360:1366	arg1	weight					1371:1376	weight	1371:1376	weight	1371:1376	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	9	62	theme	postoperative	1568:1580	arg1	inflammation					1582:1593	postoperative inflammation	1568:1593	postoperative inflammation	1568:1593	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	6	63	theme	typical	829:835	arg1	pH					825:826	neutral pH	817:826	neutral pH (typical for noninflamed tissues)	817:860	Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes.					
29406618	8	64	theme	uncontrolled	1347:1358	arg1	changes					1360:1366	no uncontrolled changes	1344:1366	no uncontrolled changes in weight and volume	1344:1387	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	64	theme	uncontrolled	1347:1358	arg1	porosity					1390:1397	porosity	1390:1397	porosity	1390:1397	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	8	64	theme	uncontrolled	1347:1358	arg1	properties					1414:1423	mechanical properties	1403:1423	mechanical properties of the composite	1403:1440	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	9	65	theme	composite	1529:1537	arg1	effects					1507:1513	volume change-related side effects	1480:1513	volume change-related side effects of HAp/glucan composite	1480:1537	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	9	66	theme	volume	1480:1485	arg1	effects					1507:1513	volume change-related side effects	1480:1513	volume change-related side effects of HAp/glucan composite	1480:1537	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	7	67	from	composite	1004:1012	arg1	serum					1049:1053	human serum	1043:1053	human serum of neutral pH	1043:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	8	68	theme	healthy	1322:1328	arg1	people					1330:1335	healthy people	1322:1335	healthy people	1322:1335	Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite.					
29406618	9	69	from	lack	1472:1475	arg1	bone					1542:1545	bone	1542:1545	bone	1542:1545	Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented.					
29406618	7	70	theme	mercury	1228:1234	arg1	intrusion					1236:1244	mercury intrusion	1228:1244	mercury intrusion	1228:1244	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	70	theme	mercury	1228:1234	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	11	71	theme	Part	1659:1662	arg1	B					1664:1664	Part B	1659:1664	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.	1640:1703	J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.					
29406618	7	72	theme	X-ray	1142:1146	arg1	diffraction					1148:1158	X-ray diffraction	1142:1158	X-ray diffraction	1142:1158	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	72	theme	X-ray	1142:1146	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	73	theme	physicochemical	970:984	arg1	parameters					986:995	physicochemical parameters	970:995	physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH	970:1067	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	74	theme	biochemical	1251:1261	arg1	weight					1081:1086	weight	1081:1086	weight	1081:1086	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29406618	7	74	theme	biochemical	1251:1261	arg1	techniques					1263:1272	biochemical techniques	1251:1272	biochemical techniques	1251:1272	Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques.					
29505709	5	0	theme	sheet	743:747	arg1	composition					724:734	the composition	720:734	the composition of the sheet	720:747	In the second hydrogel type, the composition of the sheet changed orthogonally to the direction of CNC alignment.					
29505709	4	1	theme	CNC	676:678	arg1	alignment					680:688	CNC alignment	676:688	CNC alignment	676:688	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	1	2	theme	tissues	159:165	arg1	function					131:138	the function	127:138	the function of many biological tissues	127:165	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	7	3	theme	regenerative	1073:1084	arg1	medicine					1086:1093	regenerative medicine	1073:1093	regenerative medicine	1073:1093	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	3	4	with	hydrogels	508:516	arg1	composition					484:494	a uniform composition	474:494	a uniform composition	474:494	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	3	4	with	hydrogels	508:516	arg1	types					537:541	two different types	523:541	two different types of compositional gradients	523:568	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	4	5	theme	alignment	680:688	arg1	direction					663:671	the direction	659:671	the direction of CNC alignment	659:688	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	4	6	theme	first	578:582	arg1	type					584:587	the first type	574:587	the first type of gradient hydrogel	574:608	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	1	7	theme	Compositional	66:78	arg1	patterns					95:102	Compositional and structural patterns	66:102	Compositional and structural patterns	66:102	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	4	8	theme	gradient	592:599	arg1	hydrogel					601:608	gradient hydrogel	592:608	gradient hydrogel	592:608	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	3	9	theme	compositional	546:558	arg1	gradients					560:568	compositional gradients	546:568	compositional gradients	546:568	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	3	10	theme	uniform	476:482	arg1	composition					484:494	a uniform composition	474:494	a uniform composition	474:494	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	1	11	theme	structural	84:93	arg1	patterns					95:102	Compositional and structural patterns	66:102	Compositional and structural patterns	66:102	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	2	12	theme	microextrusion-based	305:324	arg1	method					338:343	a microextrusion-based 3D printing method	303:343	a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion	303:444	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	5	13	theme	CNC	790:792	arg1	alignment					794:802	CNC alignment	790:802	CNC alignment	790:802	In the second hydrogel type, the composition of the sheet changed orthogonally to the direction of CNC alignment.					
29505709	0	14	theme	Anisotropic	27:37	arg1	Sheets					58:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Patterning of Structurally Anisotropic Composite Hydrogel Sheets.					
29505709	3	15	with	hydrogels	459:467	arg1	composition					484:494	a uniform composition	474:494	a uniform composition	474:494	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	3	15	with	hydrogels	459:467	arg1	types					537:541	two different types	523:541	two different types of compositional gradients	523:568	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	3	16	theme	gradients	560:568	arg1	types					537:541	two different types	523:541	two different types of compositional gradients	523:568	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	2	17	theme	extrusion	436:444	arg1	direction					423:431	the direction	419:431	the direction of extrusion	419:444	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	7	18	theme	tissue	1050:1055	arg1	engineering					1057:1067	tissue engineering	1050:1067	tissue engineering	1050:1067	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	3	19	theme	different	527:535	arg1	types					537:541	two different types	523:541	two different types of compositional gradients	523:568	We prepared hydrogels with a uniform composition, as well as hydrogels with two different types of compositional gradients.					
29505709	6	20	theme	mechanical	853:862	arg1	properties					864:873	mechanical properties	853:873	mechanical properties	853:873	The hydrogels exhibited gradients in structure, mechanical properties, and permeability, all governed by the compositional patterns, as well as cytocompatibility.					
29505709	1	21	theme	many	143:146	arg1	tissues					159:165	many biological tissues	143:165	many biological tissues	143:165	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	0	22	theme	Hydrogel	49:56	arg1	Sheets					58:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Patterning of Structurally Anisotropic Composite Hydrogel Sheets.					
29505709	5	23	theme	second	698:703	arg1	type					714:717	the second hydrogel type	694:717	the second hydrogel type	694:717	In the second hydrogel type, the composition of the sheet changed orthogonally to the direction of CNC alignment.					
29505709	2	24	theme	present	175:181	arg1	work					183:186	the present work	171:186	the present work	171:186	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	25	theme	cross-linked	238:249	arg1	nanocrystals					261:272	chemically cross-linked cellulose nanocrystals	227:272	chemically cross-linked cellulose nanocrystals (CNC)	227:278	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	25	theme	cross-linked	238:249	arg1	CNC					275:277	CNC	275:277	CNC	275:277	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	0	26	theme	Composite	39:47	arg1	Sheets					58:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Structurally Anisotropic Composite Hydrogel Sheets	14:63	Patterning of Structurally Anisotropic Composite Hydrogel Sheets.					
29505709	5	27	theme	hydrogel	705:712	arg1	type					714:717	the second hydrogel type	694:717	the second hydrogel type	694:717	In the second hydrogel type, the composition of the sheet changed orthogonally to the direction of CNC alignment.					
29505709	5	28	theme	alignment	794:802	arg1	direction					777:785	the direction	773:785	the direction of CNC alignment	773:802	In the second hydrogel type, the composition of the sheet changed orthogonally to the direction of CNC alignment.					
29505709	4	29	theme	sheet	634:638	arg1	composition					615:625	the composition	611:625	the composition of the sheet	611:638	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	6	30	from	gradients	829:837	arg1	structure					842:850	structure	842:850	structure	842:850	The hydrogels exhibited gradients in structure, mechanical properties, and permeability, all governed by the compositional patterns, as well as cytocompatibility.					
29505709	6	30	from	gradients	829:837	arg1	properties					864:873	mechanical properties	853:873	mechanical properties	853:873	The hydrogels exhibited gradients in structure, mechanical properties, and permeability, all governed by the compositional patterns, as well as cytocompatibility.					
29505709	6	30	from	gradients	829:837	arg1	permeability					880:891	permeability	880:891	permeability	880:891	The hydrogels exhibited gradients in structure, mechanical properties, and permeability, all governed by the compositional patterns, as well as cytocompatibility.					
29505709	0	31	theme	Sheets	58:63	arg1	Patterning					0:9	Patterning	0:9	Patterning of Structurally Anisotropic Composite Hydrogel Sheets.	0:64	Patterning of Structurally Anisotropic Composite Hydrogel Sheets.					
29505709	6	32	theme	compositional	914:926	arg1	patterns					928:935	the compositional patterns	910:935	the compositional patterns	910:935	The hydrogels exhibited gradients in structure, mechanical properties, and permeability, all governed by the compositional patterns, as well as cytocompatibility.					
29505709	1	33	theme	biological	148:157	arg1	tissues					159:165	many biological tissues	143:165	many biological tissues	143:165	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	7	34	theme	promising	989:997	arg1	applications					999:1010	promising applications	989:1010	promising applications for both fundamental research and for tissue engineering and regenerative medicine	989:1093	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	7	35	theme	fundamental	1021:1031	arg1	research					1033:1040	both fundamental research	1016:1040	both fundamental research	1016:1040	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	2	36	theme	nanofibrillar	193:205	arg1	hydrogels					207:215	nanofibrillar hydrogels	193:215	nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin	193:290	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	37	theme	printing	329:336	arg1	method					338:343	a microextrusion-based 3D printing method	303:343	a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion	303:444	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	1	38	theme	crucial	111:117	arg1	role					119:122	a crucial role	109:122	a crucial role	109:122	Compositional and structural patterns play a crucial role in the function of many biological tissues.					
29505709	7	39	contain	have	984:987	arg1	hydrogels					974:982	These hydrogels	968:982	These hydrogels	968:982	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	7	39	contain	have	984:987	arg2	applications					999:1010	promising applications	989:1010	promising applications for both fundamental research and for tissue engineering and regenerative medicine	989:1093	These hydrogels have promising applications for both fundamental research and for tissue engineering and regenerative medicine.					
29505709	2	40	theme	3D	326:327	arg1	method					338:343	a microextrusion-based 3D printing method	303:343	a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion	303:444	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	4	41	theme	hydrogel	601:608	arg1	type					584:587	the first type	574:587	the first type of gradient hydrogel	574:608	In the first type of gradient hydrogel, the composition of the sheet varied parallel to the direction of CNC alignment.					
29505709	2	42	theme	hydrogel	382:389	arg1	sheets					391:396	structurally anisotropic hydrogel sheets	357:396	structurally anisotropic hydrogel sheets	357:396	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	43	link	cross-linked	238:249	arg1	nanocrystals					261:272	chemically cross-linked cellulose nanocrystals	227:272	chemically cross-linked cellulose nanocrystals (CNC)	227:278	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	43	link	cross-linked	238:249	arg1	CNC					275:277	CNC	275:277	CNC	275:277	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	44	theme	cellulose	251:259	arg1	nanocrystals					261:272	chemically cross-linked cellulose nanocrystals	227:272	chemically cross-linked cellulose nanocrystals (CNC)	227:278	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	44	theme	cellulose	251:259	arg1	CNC					275:277	CNC	275:277	CNC	275:277	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
29505709	2	45	theme	anisotropic	370:380	arg1	sheets					391:396	structurally anisotropic hydrogel sheets	357:396	structurally anisotropic hydrogel sheets	357:396	In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion.					
31120985	10	0	theme	sudangrass	1508:1517	arg1	accessions					1519:1528	sudangrass accessions	1508:1528	sudangrass accessions	1508:1528	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31120985	2	1	theme	biotic	561:566	arg1	stresses					580:587	biotic and abiotic stresses	561:587	biotic and abiotic stresses	561:587	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	2	from	collection	365:374	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	2	from	collection	365:374	arg1	growth					310:315	potential growth	300:315	potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	300:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	3	theme	potential	300:308	arg1	growth					310:315	potential growth	300:315	potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	300:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	5	4	theme	galacturonic	1076:1087	arg1	sugars					1025:1030	the total sugars	1015:1030	the total sugars	1015:1030	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	4	theme	galacturonic	1076:1087	arg1	acid					1089:1092	galacturonic acid	1076:1092	galacturonic acid	1076:1092	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	4	5	theme	matrix	801:806	arg1	polysaccharides					808:822	matrix polysaccharides and crystalline cellulose	801:848	polysaccharides	808:822	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	4	6	theme	crystalline	828:838	arg1	cellulose					840:848	crystalline cellulose	828:848	crystalline cellulose	828:848	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	2	7	from	sudangrass	267:276	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	12	8	theme	biomass	1866:1872	arg1	composition					1847:1857	the composition	1843:1857	the composition of the biomass	1843:1872	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	12	8	theme	biomass	1866:1872	arg1	response					1882:1889	the response	1878:1889	the response to stress	1878:1899	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	12	9	theme	abiotic	1783:1789	arg1	stresses					1802:1809	abiotic and biotic stresses	1783:1809	abiotic and biotic stresses	1783:1809	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	1	10	used	used	171:174	arg2	crop					147:150	a vigorous forage crop	129:150	a vigorous forage crop that has also been used for biogas, paper, and electricity production	129:220	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	1	10	used	used	171:174	arg2	Sudangrass					81:90	Sudangrass	81:90	Sudangrass	81:90	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	12	11	theme	major	1680:1684	arg1	polymers					1696:1703	major cell wall polymers	1680:1703	major cell wall polymers	1680:1703	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	4	12	theme	bioethanol	897:906	arg1	production					908:917	bioethanol production	897:917	bioethanol production	897:917	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	3	13	theme	wall	677:680	arg1	polymers					682:689	major cell wall polymers	666:689	major cell wall polymers	666:689	The biomass composition was analyzed for major cell wall polymers, monosaccharides, and elemental composition.					
31120985	2	14	theme	large	234:238	arg1	biomass					240:246	the large biomass	230:246	the large biomass	230:246	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	15	from	area	292:295	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	1	16	theme	vigorous	131:138	arg1	crop					147:150	a vigorous forage crop	129:150	a vigorous forage crop that has also been used for biogas, paper, and electricity production	129:220	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	1	16	theme	vigorous	131:138	arg1	Sudangrass					81:90	Sudangrass	81:90	Sudangrass	81:90	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	9	17	theme	Alkaline	1364:1371	arg1	pretreatment					1373:1384	Alkaline pretreatment	1364:1384	Alkaline pretreatment	1364:1384	Alkaline pretreatment was more effective than water pretreatment.					
31120985	9	18	theme	water	1410:1414	arg1	pretreatment					1416:1427	water pretreatment	1410:1427	water pretreatment	1410:1427	Alkaline pretreatment was more effective than water pretreatment.					
31120985	5	19	theme	predominant	935:945	arg1	Xylose					920:925	Xylose	920:925	Xylose	920:925	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	19	theme	predominant	935:945	arg1	monosaccharide					969:982	the predominant matrix polysaccharide monosaccharide	931:982	the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose	931:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	12	20	theme	tolerant	1744:1751	arg1	accessions					1769:1778	tolerant and susceptible accessions	1744:1778	tolerant and susceptible accessions	1744:1778	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	2	21	theme	bioethanol	468:477	arg1	production					479:488	lignocellulosic bioethanol production	452:488	lignocellulosic bioethanol production	452:488	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	0	22	from	feedstock	43:51	arg1	Argentina					70:78	Argentina	70:78	Argentina	70:78	Sudangrass, an alternative lignocellulosic feedstock for bioenergy in Argentina.					
31120985	5	23	theme	matrix	947:952	arg1	Xylose					920:925	Xylose	920:925	Xylose	920:925	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	23	theme	matrix	947:952	arg1	monosaccharide					969:982	the predominant matrix polysaccharide monosaccharide	931:982	the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose	931:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	2	24	theme	sudangrass	336:345	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	3	25	theme	cell	672:675	arg1	polymers					682:689	major cell wall polymers	666:689	major cell wall polymers	666:689	The biomass composition was analyzed for major cell wall polymers, monosaccharides, and elemental composition.					
31120985	2	26	theme	abiotic	572:578	arg1	stresses					580:587	biotic and abiotic stresses	561:587	biotic and abiotic stresses	561:587	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	12	27	theme	composition	1724:1734	arg1	comparison					1666:1675	The comparison	1662:1675	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses	1662:1809	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	1	28	theme	Sorghum	93:99	arg1	Stapf					119:123	Sorghum sudanense (Piper) Stapf	93:123	Sorghum sudanense (Piper) Stapf	93:123	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	1	28	theme	Sorghum	93:99	arg1	Sudangrass					81:90	Sudangrass	81:90	Sudangrass	81:90	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	2	29	theme	Argentina	320:328	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	30	theme	sudanense	382:390	arg1	collection					365:374	a collection	363:374	a collection of S. sudanense	363:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	31	theme	biomass	616:622	arg1	response					549:556	the response	545:556	the response to biotic and abiotic stresses	545:587	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	31	theme	biomass	616:622	arg1	composition					597:607	the composition	593:607	the composition of the biomass	593:622	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	1	32	theme	sudanense	101:109	arg1	Stapf					119:123	Sorghum sudanense (Piper) Stapf	93:123	Sorghum sudanense (Piper) Stapf	93:123	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	1	32	theme	sudanense	101:109	arg1	Sudangrass					81:90	Sudangrass	81:90	Sudangrass	81:90	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	7	33	from	element	1222:1228	arg1	stem					1244:1247	sudangrass stem	1233:1247	sudangrass stem	1233:1247	Silica was the most abundant element in sudangrass stem, followed by chloride, calcium, phosphorus and sulfur.					
31120985	2	34	theme	S.	379:380	arg1	sudanense					382:390	S. sudanense	379:390	S. sudanense	379:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	5	35	theme	sugars	1025:1030	arg1	%					1010:1010	45%	1008:1010	45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose	1008:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	acid					1115:1118	glucuronic acid	1104:1118	glucuronic acid	1104:1118	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	acid					1089:1092	galacturonic acid	1076:1092	galacturonic acid	1076:1092	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	fucose					1125:1130	fucose	1125:1130	fucose	1125:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	glucose					1056:1062	glucose	1056:1062	glucose	1056:1062	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	mannose					1095:1101	mannose	1095:1101	mannose	1095:1101	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	sugars					1025:1030	the total sugars	1015:1030	the total sugars	1015:1030	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	35	theme	sugars	1025:1030	arg1	galactose					1065:1073	galactose	1065:1073	galactose	1065:1073	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	7	36	theme	sudangrass	1233:1242	arg1	stem					1244:1247	sudangrass stem	1233:1247	sudangrass stem	1233:1247	Silica was the most abundant element in sudangrass stem, followed by chloride, calcium, phosphorus and sulfur.					
31120985	10	37	theme	hydroxide	1437:1445	arg1	pretreatment					1447:1458	Sodium hydroxide pretreatment	1430:1458	Sodium hydroxide pretreatment	1430:1458	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31120985	12	38	theme	cell	1686:1689	arg1	polymers					1696:1703	major cell wall polymers	1680:1703	major cell wall polymers	1680:1703	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	4	39	theme	stem	755:758	arg1	biomass					776:782	stem lignocellulosic biomass	755:782	stem lignocellulosic biomass	755:782	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	7	40	theme	abundant	1213:1220	arg1	element					1222:1228	the most abundant element	1204:1228	the most abundant element in sudangrass stem	1204:1247	Silica was the most abundant element in sudangrass stem, followed by chloride, calcium, phosphorus and sulfur.					
31120985	7	40	theme	abundant	1213:1220	arg1	Silica					1193:1198	Silica	1193:1198	Silica	1193:1198	Silica was the most abundant element in sudangrass stem, followed by chloride, calcium, phosphorus and sulfur.					
31120985	0	41	theme	lignocellulosic	27:41	arg1	feedstock					43:51	an alternative lignocellulosic feedstock	12:51	an alternative lignocellulosic feedstock for bioenergy in Argentina	12:78	Sudangrass, an alternative lignocellulosic feedstock for bioenergy in Argentina.					
31120985	0	41	theme	lignocellulosic	27:41	arg1	Sudangrass					0:9	Sudangrass	0:9	Sudangrass	0:9	Sudangrass, an alternative lignocellulosic feedstock for bioenergy in Argentina.					
31120985	4	42	theme	sugars	886:891	arg1	source					876:881	a potential source	864:881	a potential source of sugars for bioethanol production	864:917	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	0	43	theme	alternative	15:25	arg1	feedstock					43:51	an alternative lignocellulosic feedstock	12:51	an alternative lignocellulosic feedstock for bioenergy in Argentina	12:78	Sudangrass, an alternative lignocellulosic feedstock for bioenergy in Argentina.					
31120985	0	43	theme	alternative	15:25	arg1	Sudangrass					0:9	Sudangrass	0:9	Sudangrass	0:9	Sudangrass, an alternative lignocellulosic feedstock for bioenergy in Argentina.					
31120985	5	44	theme	glucuronic	1104:1113	arg1	sugars					1025:1030	the total sugars	1015:1030	the total sugars	1015:1030	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	44	theme	glucuronic	1104:1113	arg1	acid					1115:1118	glucuronic acid	1104:1118	glucuronic acid	1104:1118	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	10	45	theme	recalcitrance	1488:1500	arg1	levels					1478:1483	different levels	1468:1483	different levels of recalcitrance among sudangrass accessions	1468:1528	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31120985	11	46	theme	Phenological	1571:1582	arg1	traits					1584:1589	Phenological traits	1571:1589	Phenological traits	1571:1589	Phenological traits were also evaluated, showing significant variability among accessions.					
31120985	1	47	dep	crop	147:150	arg1	forage					140:145	forage	140:145	forage	140:145	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	3	48	theme	major	666:670	arg1	polymers					682:689	major cell wall polymers	666:689	major cell wall polymers	666:689	The biomass composition was analyzed for major cell wall polymers, monosaccharides, and elemental composition.					
31120985	1	49	theme	Piper	112:116	arg1	Stapf					119:123	Sorghum sudanense (Piper) Stapf	93:123	Sorghum sudanense (Piper) Stapf	93:123	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	1	49	theme	Piper	112:116	arg1	Sudangrass					81:90	Sudangrass	81:90	Sudangrass	81:90	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	10	50	theme	Sodium	1430:1435	arg1	pretreatment					1447:1458	Sodium hydroxide pretreatment	1430:1458	Sodium hydroxide pretreatment	1430:1458	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31120985	3	51	theme	elemental	713:721	arg1	composition					723:733	elemental composition	713:733	elemental composition	713:733	The biomass composition was analyzed for major cell wall polymers, monosaccharides, and elemental composition.					
31120985	4	52	theme	lignocellulosic	760:774	arg1	biomass					776:782	stem lignocellulosic biomass	755:782	stem lignocellulosic biomass	755:782	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	2	53	from	accessions	347:356	arg1	area					292:295	the large area	282:295	the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	282:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	53	from	accessions	347:356	arg1	sudangrass					267:276	sudangrass	267:276	sudangrass	267:276	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	54	theme	large	286:290	arg1	area					292:295	the large area	282:295	the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	282:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	12	55	theme	polymers	1696:1703	arg1	comparison					1666:1675	The comparison	1662:1675	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses	1662:1809	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	2	56	from	growth	310:315	arg1	accessions					347:356	Argentina seven sudangrass accessions	320:356	Argentina seven sudangrass accessions from a collection of S. sudanense	320:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	56	from	growth	310:315	arg1	collection					365:374	a collection	363:374	a collection of S. sudanense	363:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	4	57	theme	potential	866:874	arg1	source					876:881	a potential source	864:881	a potential source of sugars for bioethanol production	864:917	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	3	58	theme	biomass	629:635	arg1	composition					637:647	The biomass composition	625:647	The biomass composition	625:647	The biomass composition was analyzed for major cell wall polymers, monosaccharides, and elemental composition.					
31120985	2	59	theme	Due	223:225	arg1	yields					248:253	Due to the large biomass yields	223:253	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	223:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	12	60	theme	susceptible	1757:1767	arg1	accessions					1769:1778	tolerant and susceptible accessions	1744:1778	tolerant and susceptible accessions	1744:1778	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	12	61	theme	wall	1691:1694	arg1	polymers					1696:1703	major cell wall polymers	1680:1703	major cell wall polymers	1680:1703	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	10	62	theme	different	1468:1476	arg1	levels					1478:1483	different levels	1468:1483	different levels of recalcitrance among sudangrass accessions	1468:1528	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31120985	1	63	dep	biogas	180:185	arg1	production					211:220	production	211:220	production	211:220	Sudangrass, Sorghum sudanense (Piper) Stapf, is a vigorous forage crop that has also been used for biogas, paper, and electricity production.					
31120985	12	64	theme	biotic	1795:1800	arg1	stresses					1802:1809	abiotic and biotic stresses	1783:1809	abiotic and biotic stresses	1783:1809	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	12	65	theme	monosaccharide	1709:1722	arg1	composition					1724:1734	monosaccharide composition	1709:1734	monosaccharide composition	1709:1734	The comparison of major cell wall polymers and monosaccharide composition between tolerant and susceptible accessions to abiotic and biotic stresses suggests an association between the composition of the biomass and the response to stress.					
31120985	2	66	theme	lignocellulosic	452:466	arg1	production					479:488	lignocellulosic bioethanol production	452:488	lignocellulosic bioethanol production	452:488	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	5	67	theme	total	1019:1023	arg1	acid					1115:1118	glucuronic acid	1104:1118	glucuronic acid	1104:1118	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	acid					1089:1092	galacturonic acid	1076:1092	galacturonic acid	1076:1092	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	fucose					1125:1130	fucose	1125:1130	fucose	1125:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	glucose					1056:1062	glucose	1056:1062	glucose	1056:1062	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	mannose					1095:1101	mannose	1095:1101	mannose	1095:1101	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	sugars					1025:1030	the total sugars	1015:1030	the total sugars	1015:1030	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	67	theme	total	1019:1023	arg1	galactose					1065:1073	galactose	1065:1073	galactose	1065:1073	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	4	68	theme	biomass	776:782	arg1	%					750:750	68%	748:750	68% of stem lignocellulosic biomass	748:782	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	4	68	theme	biomass	776:782	arg1	biomass					776:782	stem lignocellulosic biomass	755:782	stem lignocellulosic biomass	755:782	On average, 68% of stem lignocellulosic biomass was comprised of matrix polysaccharides and crystalline cellulose, representing a potential source of sugars for bioethanol production.					
31120985	8	69	theme	saccharification	1317:1332	arg1	analyses					1334:1341	saccharification analyses	1317:1341	saccharification analyses	1317:1341	We performed saccharification analyses after pretreatments.					
31120985	5	70	theme	polysaccharide	954:967	arg1	Xylose					920:925	Xylose	920:925	Xylose	920:925	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	5	70	theme	polysaccharide	954:967	arg1	monosaccharide					969:982	the predominant matrix polysaccharide monosaccharide	931:982	the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose	931:1130	Xylose was the predominant matrix polysaccharide monosaccharide comprising, on average, 45% of the total sugars, followed by arabinose, glucose, galactose, galacturonic acid, mannose, glucuronic acid, and fucose.					
31120985	11	71	theme	significant	1620:1630	arg1	variability					1632:1642	significant variability	1620:1642	significant variability among accessions	1620:1659	Phenological traits were also evaluated, showing significant variability among accessions.					
31120985	6	72	located	detected	1150:1157	arg1	any					1162:1164	any	1162:1164	any	1162:1164	Rhamnose was not detected in any of the biomasses analyzed.					
31120985	6	72	located	detected	1150:1157	arg2	Rhamnose					1133:1140	Rhamnose	1133:1140	Rhamnose	1133:1140	Rhamnose was not detected in any of the biomasses analyzed.					
31120985	2	73	theme	growth	310:315	arg1	area					292:295	the large area	282:295	the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense	282:390	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	2	73	theme	growth	310:315	arg1	sudangrass					267:276	sudangrass	267:276	sudangrass	267:276	Due to the large biomass yields achieved by sudangrass and the large area of potential growth in Argentina seven sudangrass accessions from a collection of S. sudanense were analyzed to evaluate their potential as feedstocks for lignocellulosic bioethanol production, and to assess whether there is an association between the response to biotic and abiotic stresses and the composition of the biomass.					
31120985	10	74	theme	water	1543:1547	arg1	pretreatment					1549:1560	the water pretreatment	1539:1560	the water pretreatment	1539:1560	Sodium hydroxide pretreatment exposed different levels of recalcitrance among sudangrass accessions, whereas the water pretreatment did not.					
31373288	4	0	theme	fiber	904:908	arg1	content					915:921	the dietary fiber (DF) content	892:921	the dietary fiber (DF) content	892:921	In addition, the dietary fiber (DF) content was also evaluated.					
31373288	3	1	theme	thermal	660:666	arg1	properties					740:749	thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties	660:749	thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules	660:790	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	5	2	theme	G/CM	1054:1057	arg1	ratio					1059:1063	the G/CM ratio	1050:1063	the G/CM ratio of 3:1	1050:1070	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	5	2	theme	G/CM	1054:1057	arg1	suitable					1078:1085	suitable	1078:1085	suitable	1078:1085	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	10	3	theme	G	1572:1572	arg1	formulation					1550:1560	a formulation	1548:1560	a formulation of CM and G	1548:1572	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	3	4	theme	control	825:831	arg1	capsules					838:845	control (CC) capsules	825:845	control (CC) capsules containing only G and glycerol	825:876	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	5	dep	transform	603:611	arg1	infrared					613:620	infrared	613:620	transform infrared	603:620	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	4	6	theme	dietary	896:902	arg1	content					915:921	the dietary fiber (DF) content	892:921	the dietary fiber (DF) content	892:921	In addition, the dietary fiber (DF) content was also evaluated.					
31373288	3	7	dep	microscopy	547:556	arg1	SEM					559:561	SEM	559:561	SEM	559:561	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	10	8	theme	softgels	1533:1540	arg1	feasibility					1507:1517	the feasibility	1503:1517	the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties	1503:1617	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	5	9	theme	composite	987:995	arg1	samples					997:1003	composite samples	987:1003	composite samples	987:1003	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	3	10	theme	CMC	778:780	arg1	capsules					783:790	the prepared composite (CMC) capsules	754:790	the prepared composite (CMC) capsules	754:790	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	5	11	dep	decreased	1005:1013	arg1	ratio					1059:1063	the G/CM ratio	1050:1063	the G/CM ratio of 3:1	1050:1070	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	5	11	dep	decreased	1005:1013	arg1	suitable					1078:1085	suitable	1078:1085	suitable	1078:1085	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	5	12	theme	CM	1046:1047	arg1	presence					1034:1041	the presence	1030:1041	the presence of CM	1030:1047	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	7	13	theme	CMC	1298:1300	arg1	morphology					1263:1272	morphology	1263:1272	morphology	1263:1272	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	7	13	theme	CMC	1298:1300	arg1	behavior					1286:1293	thermal behavior	1278:1293	thermal behavior	1278:1293	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	3	14	theme	prepared	758:765	arg1	capsules					783:790	the prepared composite (CMC) capsules	754:790	the prepared composite (CMC) capsules	754:790	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	15	from	characterization	577:592	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	15	from	characterization	577:592	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	5	16	theme	samples	997:1003	arg1	transparency					971:982	the transparency	967:982	the transparency of composite samples	967:1003	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	10	17	theme	nutritional	1596:1606	arg1	properties					1608:1617	nutritional properties	1596:1617	nutritional properties	1596:1617	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	3	18	theme	composite	767:775	arg1	capsules					783:790	the prepared composite (CMC) capsules	754:790	the prepared composite (CMC) capsules	754:790	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	7	19	contain	had	1232:1234	arg2	effects					1248:1254	significant effects	1236:1254	significant effects on the morphology and thermal behavior of CMC	1236:1300	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	7	19	contain	had	1232:1234	arg1	presence					1205:1212	the presence	1201:1212	the presence of polysaccharide	1201:1230	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	10	20	theme	CM	1565:1566	arg1	formulation					1550:1560	a formulation	1548:1560	a formulation of CM and G	1548:1572	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	8	21	theme	CM	1386:1387	arg1	biopolymers					1395:1405	CM and G biopolymers	1386:1405	biopolymers	1395:1405	FTIR spectra confirmed the CMC formation by crosslinking between CM and G biopolymers.					
31373288	2	22	theme	different	399:407	arg1	ratios					414:419	different G/CM ratios	399:419	different G/CM ratios (3:1, 1:1 and 1:3)	399:438	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	8	23	theme	G	1393:1393	arg1	biopolymers					1395:1405	CM and G biopolymers	1386:1405	biopolymers	1395:1405	FTIR spectra confirmed the CMC formation by crosslinking between CM and G biopolymers.					
31373288	4	24	theme	DF	911:912	arg1	content					915:921	the dietary fiber (DF) content	892:921	the dietary fiber (DF) content	892:921	In addition, the dietary fiber (DF) content was also evaluated.					
31373288	3	25	from	Transparency	454:465	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	25	from	Transparency	454:465	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	26	theme	calorimetry/thermogravimetric	691:719	arg1	analysis					721:728	differential scanning calorimetry/thermogravimetric analysis	669:728	differential scanning calorimetry/thermogravimetric analysis	669:728	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	26	theme	calorimetry/thermogravimetric	691:719	arg1	DSC/TGA					731:737	DSC/TGA	731:737	DSC/TGA	731:737	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	1	27	dep	gelatin	181:187	arg1	biopolymers					218:228	biopolymers	218:228	biopolymers	218:228	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	3	28	theme	CC	834:835	arg1	capsules					838:845	control (CC) capsules	825:845	control (CC) capsules containing only G and glycerol	825:876	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	2	29	theme	concentration	382:394	arg1	effect					370:375	The effect	366:375	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3)	366:438	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	3	30	theme	differential	669:680	arg1	analysis					721:728	differential scanning calorimetry/thermogravimetric analysis	669:728	differential scanning calorimetry/thermogravimetric analysis	669:728	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	30	theme	differential	669:680	arg1	DSC/TGA					731:737	DSC/TGA	731:737	DSC/TGA	731:737	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	6	31	theme	CM	1125:1126	arg1	addition					1113:1120	The addition	1109:1120	The addition of CM	1109:1126	The addition of CM decreased the MC, the SW and the lightness of the capsules.					
31373288	8	32	theme	CMC	1348:1350	arg1	formation					1352:1360	the CMC formation	1344:1360	the CMC formation	1344:1360	FTIR spectra confirmed the CMC formation by crosslinking between CM and G biopolymers.					
31373288	7	33	theme	thermal	1278:1284	arg1	behavior					1286:1293	thermal behavior	1278:1293	thermal behavior	1278:1293	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	2	34	theme	G	380:380	arg1	concentration					382:394	G concentration	380:394	G concentration	380:394	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	3	35	theme	scanning	682:689	arg1	analysis					721:728	differential scanning calorimetry/thermogravimetric analysis	669:728	differential scanning calorimetry/thermogravimetric analysis	669:728	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	35	theme	scanning	682:689	arg1	DSC/TGA					731:737	DSC/TGA	731:737	DSC/TGA	731:737	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	36	from	content	477:483	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	36	from	content	477:483	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	37	theme	electron	538:545	arg1	microscopy					547:556	scanning electron microscopy	529:556	scanning electron microscopy	529:556	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	37	theme	electron	538:545	arg1	morphology					517:526	morphology	517:526	morphology (scanning electron microscopy, SEM)	517:562	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	0	38	theme	Physicochemical	16:30	arg1	Characterization					32:47	Physicochemical Characterization	16:47	Physicochemical Characterization	16:47	Preparation and Physicochemical Characterization of Softgels Cross-Linked with Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica.					
31373288	1	39	theme	alternative	296:306	arg1	formulation					163:173	A new crosslinking formulation	144:173	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers	144:228	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	39	theme	alternative	296:306	arg1	material					313:320	an alternative wall material	293:320	an alternative wall material to traditional gelatin capsules (softgels)	293:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	3	40	contain	containing	847:856	arg2	glycerol					869:876	glycerol	869:876	glycerol	869:876	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	40	contain	containing	847:856	arg1	capsules					838:845	control (CC) capsules	825:845	control (CC) capsules containing only G and glycerol	825:876	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	40	contain	containing	847:856	arg2	G					863:863	G	863:863	G	863:863	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	1	41	dep	developed	234:242	arg1	characterized					263:275	characterized	263:275	characterized	263:275	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	41	dep	developed	234:242	arg1	proposed					281:288	proposed	281:288	proposed as an alternative wall material to traditional gelatin capsules (softgels)	281:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	7	42	from	effects	1248:1254	arg1	morphology					1263:1272	morphology	1263:1272	morphology	1263:1272	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	7	42	from	effects	1248:1254	arg1	behavior					1286:1293	thermal behavior	1278:1293	thermal behavior	1278:1293	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	3	43	dep	thermal	660:666	arg1	analysis					721:728	differential scanning calorimetry/thermogravimetric analysis	669:728	differential scanning calorimetry/thermogravimetric analysis	669:728	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	43	dep	thermal	660:666	arg1	DSC/TGA					731:737	DSC/TGA	731:737	DSC/TGA	731:737	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	0	44	theme	Softgels	52:59	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Physicochemical Characterization of Softgels Cross-Linked with Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica.					
31373288	0	44	theme	Softgels	52:59	arg1	Characterization					32:47	Physicochemical Characterization	16:47	Physicochemical Characterization	16:47	Preparation and Physicochemical Characterization of Softgels Cross-Linked with Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica.					
31373288	1	45	theme	wall	308:311	arg1	formulation					163:173	A new crosslinking formulation	144:173	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers	144:228	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	45	theme	wall	308:311	arg1	material					313:320	an alternative wall material	293:320	an alternative wall material to traditional gelatin capsules (softgels)	293:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	2	46	dep	ratios	414:419	arg1	3:1					422:424	3:1	422:424	3:1	422:424	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	1	47	theme	cactus	197:202	arg1	CM					214:215	CM	214:215	CM	214:215	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	47	theme	cactus	197:202	arg1	mucilage					204:211	cactus mucilage	197:211	cactus mucilage (CM)	197:216	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	10	48	theme	developing	1522:1531	arg1	softgels					1533:1540	developing softgels	1522:1540	developing softgels using a formulation of CM and G as wall material with nutritional properties	1522:1617	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	6	49	theme	capsules	1178:1185	arg1	MC					1142:1143	the MC	1138:1143	the MC	1138:1143	The addition of CM decreased the MC, the SW and the lightness of the capsules.					
31373288	6	49	theme	capsules	1178:1185	arg1	SW					1150:1151	the SW	1146:1151	the SW	1146:1151	The addition of CM decreased the MC, the SW and the lightness of the capsules.					
31373288	6	49	theme	capsules	1178:1185	arg1	lightness					1161:1169	the lightness	1157:1169	the lightness of the capsules	1157:1185	The addition of CM decreased the MC, the SW and the lightness of the capsules.					
31373288	3	50	theme	vibrational	565:575	arg1	Fourier					595:601	Fourier	595:601	Fourier transform infrared	595:620	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	50	theme	vibrational	565:575	arg1	characterization					577:592	vibrational characterization	565:592	vibrational characterization (Fourier transform infrared, FTIR)	565:627	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	7	51	theme	significant	1236:1246	arg1	effects					1248:1254	significant effects	1236:1254	significant effects on the morphology and thermal behavior of CMC	1236:1300	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	2	52	theme	G/CM	409:412	arg1	ratios					414:419	different G/CM ratios	399:419	different G/CM ratios (3:1, 1:1 and 1:3)	399:438	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	5	53	theme	3:1	1068:1070	arg1	ratio					1059:1063	the G/CM ratio	1050:1063	the G/CM ratio of 3:1	1050:1070	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	5	53	theme	3:1	1068:1070	arg1	suitable					1078:1085	suitable	1078:1085	suitable	1078:1085	Our results showed that the transparency of composite samples decreased gradually with the presence of CM, the G/CM ratio of 3:1 being suitable to form the softgels.					
31373288	2	54	dep	3:1	422:424	arg1	1:3					435:437	1:3	435:437	1:3	435:437	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	2	54	dep	3:1	422:424	arg1	1:1					427:429	1:1	427:429	1:1	427:429	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	3	55	theme	moisture	468:475	arg1	MC					486:487	MC	486:487	MC	486:487	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	55	theme	moisture	468:475	arg1	content					477:483	moisture content	468:483	moisture content (MC)	468:488	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	56	from	parameters	636:645	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	56	from	parameters	636:645	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	1	57	theme	traditional	325:335	arg1	softgels					355:362	softgels	355:362	softgels	355:362	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	57	theme	traditional	325:335	arg1	capsules					345:352	traditional gelatin capsules	325:352	traditional gelatin capsules (softgels)	325:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	3	58	from	properties	740:749	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	58	from	properties	740:749	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	59	theme	scanning	529:536	arg1	microscopy					547:556	scanning electron microscopy	529:556	scanning electron microscopy	529:556	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	59	theme	scanning	529:536	arg1	morphology					517:526	morphology	517:526	morphology (scanning electron microscopy, SEM)	517:562	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	9	60	theme	CM	1424:1425	arg1	addition					1412:1419	The addition	1408:1419	The addition of CM to the softgels formulation	1408:1453	The addition of CM to the softgels formulation influenced the DF content.					
31373288	1	61	theme	gelatin	337:343	arg1	softgels					355:362	softgels	355:362	softgels	355:362	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	61	theme	gelatin	337:343	arg1	capsules					345:352	traditional gelatin capsules	325:352	traditional gelatin capsules (softgels)	325:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	7	62	dep	CC	1317:1318	arg1	contrast					1305:1312	contrast	1305:1312	contrast	1305:1312	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	8	63	theme	FTIR	1321:1324	arg1	spectra					1326:1332	FTIR spectra	1321:1332	FTIR spectra	1321:1332	FTIR spectra confirmed the CMC formation by crosslinking between CM and G biopolymers.					
31373288	9	64	theme	DF	1470:1471	arg1	content					1473:1479	the DF content	1466:1479	the DF content	1466:1479	The addition of CM to the softgels formulation influenced the DF content.					
31373288	1	65	theme	new	146:148	arg1	material					313:320	an alternative wall material	293:320	an alternative wall material to traditional gelatin capsules (softgels)	293:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	65	theme	new	146:148	arg1	formulation					163:173	A new crosslinking formulation	144:173	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers	144:228	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	3	66	from	morphology	517:526	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	66	from	morphology	517:526	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	7	67	theme	polysaccharide	1217:1230	arg1	presence					1205:1212	the presence	1201:1212	the presence of polysaccharide	1201:1230	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	2	68	from	ratios	414:419	arg1	effect					370:375	The effect	366:375	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3)	366:438	The effect of G concentration at different G/CM ratios (3:1, 1:1 and 1:3) was analyzed.					
31373288	1	69	theme	crosslinking	150:161	arg1	material					313:320	an alternative wall material	293:320	an alternative wall material to traditional gelatin capsules (softgels)	293:363	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	1	69	theme	crosslinking	150:161	arg1	formulation					163:173	A new crosslinking formulation	144:173	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers	144:228	A new crosslinking formulation using gelatin (G) and cactus mucilage (CM) biopolymers was developed, physicochemically characterized and proposed as an alternative wall material to traditional gelatin capsules (softgels).					
31373288	10	70	with	material	1582:1589	arg1	properties					1608:1617	nutritional properties	1596:1617	nutritional properties	1596:1617	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	10	71	theme	wall	1577:1580	arg1	material					1582:1589	wall material	1577:1589	wall material with nutritional properties	1577:1617	Our findings support the feasibility of developing softgels using a formulation of CM and G as wall material with nutritional properties.					
31373288	3	72	theme	capsules	783:790	arg1	CIELab					648:653	CIELab	648:653	CIELab	648:653	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	microscopy					547:556	scanning electron microscopy	529:556	scanning electron microscopy	529:556	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	characterization					577:592	vibrational characterization	565:592	vibrational characterization (Fourier transform infrared, FTIR)	565:627	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	MC					486:487	MC	486:487	MC	486:487	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	Transparency					454:465	Transparency	454:465	Transparency	454:465	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	Fourier					595:601	Fourier	595:601	Fourier transform infrared	595:620	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	morphology					517:526	morphology	517:526	morphology (scanning electron microscopy, SEM)	517:562	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	solubility					491:500	solubility	491:500	solubility in water (SW)	491:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	content					477:483	moisture content	468:483	moisture content (MC)	468:488	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	parameters					636:645	color parameters	630:645	color parameters (CIELab)	630:654	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	72	theme	capsules	783:790	arg1	properties					740:749	thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties	660:749	thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules	660:790	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	9	73	theme	softgels	1434:1441	arg1	formulation					1443:1453	the softgels formulation	1430:1453	the softgels formulation	1430:1453	The addition of CM to the softgels formulation influenced the DF content.					
31373288	0	74	theme	Cactus	79:84	arg1	Mucilage					86:93	Cactus Mucilage	79:93	Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica	79:141	Preparation and Physicochemical Characterization of Softgels Cross-Linked with Cactus Mucilage Extracted from Cladodes of Opuntia Ficus-Indica.					
31373288	3	75	theme	color	630:634	arg1	CIELab					648:653	CIELab	648:653	CIELab	648:653	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	75	theme	color	630:634	arg1	parameters					636:645	color parameters	630:645	color parameters (CIELab)	630:654	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	7	76	dep	morphology	1263:1272	arg1	the					1259:1261	the	1259:1261	the	1259:1261	Furthermore, the presence of polysaccharide had significant effects on the morphology and thermal behavior of CMC in contrast to CC.					
31373288	3	77	from	solubility	491:500	arg1	water					505:509	water	505:509	water (SW)	505:514	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	77	from	solubility	491:500	arg1	SW					512:513	SW	512:513	SW	512:513	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	78	dep	Fourier	595:601	arg1	transform					603:611	transform	603:611	transform infrared	603:620	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
31373288	3	78	dep	Fourier	595:601	arg1	FTIR					623:626	FTIR	623:626	FTIR	623:626	Transparency, moisture content (MC), solubility in water (SW), morphology (scanning electron microscopy, SEM), vibrational characterization (Fourier transform infrared, FTIR), color parameters (CIELab) and thermal (differential scanning calorimetry/thermogravimetric analysis, DSC/TGA) properties of the prepared composite (CMC) capsules were estimated and compared with control (CC) capsules containing only G and glycerol.					
30177186	0	0	theme	AAPH-induced	79:90	arg1	damage					102:107	AAPH-induced oxidative damage	79:107	AAPH-induced oxidative damage in human erythrocytes	79:129	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	4	1	from	arabinose	742:750	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	2	from	arabinose	561:569	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	3	theme	glucuronic	493:502	arg1	acid					504:507	glucuronic acid	493:507	glucuronic acid	493:507	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	7	4	theme	natural	1206:1212	arg1	antioxidant					1214:1224	a natural antioxidant	1204:1224	a natural antioxidant	1204:1224	The results provide an important basis for the development of S. laniceps as a natural antioxidant.					
30177186	5	5	from	GSH	940:942	arg1	erythrocytes					969:980	AAPH-treated erythrocytes	956:980	AAPH-treated erythrocytes	956:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	4	6	from	rhamnose	483:490	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	7	theme	GSH	940:942	arg1	balance					929:935	the balance	925:935	the balance of GSH and GSSG in AAPH-treated erythrocytes	925:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	0	8	theme	oxidative	92:100	arg1	damage					102:107	AAPH-induced oxidative damage	79:107	AAPH-induced oxidative damage in human erythrocytes	79:129	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	4	9	from	galactose	727:735	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	0	10	theme	human	112:116	arg1	erythrocytes					118:129	human erythrocytes	112:129	human erythrocytes	112:129	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	6	11	theme	polysaccharide	1097:1110	arg1	cells					1120:1124	polysaccharide treated cells	1097:1124	polysaccharide treated cells	1097:1124	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	4	12	from	mannose	474:480	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	13	theme	GSSG	948:951	arg1	balance					929:935	the balance	925:935	the balance of GSH and GSSG in AAPH-treated erythrocytes	925:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	5	14	theme	ROS	906:908	arg1	generation					878:887	the generation	874:887	the generation of intracellular ROS	874:908	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	0	15	from	torus	35:39	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from the torus of Saussurea laniceps	0:61	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	4	16	from	glucose	718:724	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	17	from	xylose	549:554	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	0	18	theme	novel	4:8	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from the torus of Saussurea laniceps	0:61	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	7	19	theme	S.	1189:1190	arg1	laniceps					1192:1199	S. laniceps	1189:1199	S. laniceps	1189:1199	The results provide an important basis for the development of S. laniceps as a natural antioxidant.					
30177186	5	20	from	erythrocytes	969:980	arg1	balance					929:935	the balance	925:935	the balance of GSH and GSSG in AAPH-treated erythrocytes	925:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	5	21	theme	MDA	851:853	arg1	content					855:861	MDA content	851:861	MDA content	851:861	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	7	22	theme	important	1150:1158	arg1	basis					1160:1164	an important basis	1147:1164	an important basis for the development of S. laniceps as a natural antioxidant	1147:1224	The results provide an important basis for the development of S. laniceps as a natural antioxidant.					
30177186	4	23	from	acid	712:715	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	2	24	theme	human	304:308	arg1	erythrocytes					310:321	human erythrocytes	304:321	human erythrocytes	304:321	Their molecular weight, monosaccharide compositions and the ability to protect human erythrocytes from oxidative damage induced by AAPH were assessed.					
30177186	3	25	theme	SLT-4	416:420	arg1	Mw					400:401	the Mw	396:401	the Mw of SLT-3 and SLT-4	396:420	Results showed that the Mw of SLT-3 and SLT-4 were 10,113 Da and 12,392 Da.					
30177186	3	25	theme	SLT-4	416:420	arg1	10,113 Da					427:435	10,113 Da	427:435	10,113 Da	427:435	Results showed that the Mw of SLT-3 and SLT-4 were 10,113 Da and 12,392 Da.					
30177186	4	26	from	glucose	529:535	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	1	27	dep	polysaccharides	142:156	arg1	SLT-4					166:170	SLT-4	166:170	SLT-4	166:170	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	1	27	dep	polysaccharides	142:156	arg1	SLT-3					159:163	SLT-3	159:163	SLT-3	159:163	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	4	28	theme	galacturonic	699:710	arg1	acid					712:715	galacturonic acid	699:715	galacturonic acid	699:715	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	6	29	theme	treated	1112:1118	arg1	cells					1120:1124	polysaccharide treated cells	1097:1124	polysaccharide treated cells	1097:1124	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	30	theme	enzymes	1040:1046	arg1	activities					1000:1009	the activities	996:1009	the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT,	996:1076	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	4	31	from	acid	523:526	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	32	with	Pretreatment	809:820	arg1	SLT-3					827:831	SLT-3	827:831	SLT-3	827:831	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	5	32	with	Pretreatment	809:820	arg1	SLT-4					837:841	SLT-4	837:841	SLT-4	837:841	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	6	33	theme	antioxidant	1028:1038	arg1	enzymes					1040:1046	intracellular antioxidant enzymes	1014:1046	intracellular antioxidant enzymes	1014:1046	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	33	theme	antioxidant	1028:1038	arg1	CAT					1073:1075	CAT	1073:1075	CAT	1073:1075	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	33	theme	antioxidant	1028:1038	arg1	GSH-Px					1062:1067	GSH-Px	1062:1067	GSH-Px	1062:1067	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	33	theme	antioxidant	1028:1038	arg1	SOD					1057:1059	SOD	1057:1059	SOD	1057:1059	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	3	34	theme	SLT-3	406:410	arg1	Mw					400:401	the Mw	396:401	the Mw of SLT-3 and SLT-4	396:420	Results showed that the Mw of SLT-3 and SLT-4 were 10,113 Da and 12,392 Da.					
30177186	3	34	theme	SLT-3	406:410	arg1	10,113 Da					427:435	10,113 Da	427:435	10,113 Da	427:435	Results showed that the Mw of SLT-3 and SLT-4 were 10,113 Da and 12,392 Da.					
30177186	1	35	attach	isolated	178:185	arg1	torus					196:200	torus	196:200	torus	196:200	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	1	35	attach	isolated	178:185	arg2	polysaccharides					142:156	Two major polysaccharides	132:156	Two major polysaccharides (SLT-3, SLT-4)	132:171	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	4	36	theme	0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	591:630	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	0	37	theme	Saussurea	44:52	arg1	laniceps					54:61	Saussurea laniceps	44:61	Saussurea laniceps	44:61	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	4	38	dep	glucose	529:535	arg1	composed					651:658	composed	651:658	was composed of mannose	647:669	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	39	theme	glucuronic	682:691	arg1	acid					693:696	glucuronic acid	682:696	glucuronic acid	682:696	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	40	from	acid	504:507	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	41	theme	0.92:5.61:0.93:19.50:2.42:5.27:3.01	772:806	arg1	ratio					763:767	a molar ratio	755:767	a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01	755:806	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	42	theme	intracellular	892:904	arg1	ROS					906:908	intracellular ROS	892:908	intracellular ROS	892:908	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	5	43	from	balance	929:935	arg1	erythrocytes					969:980	AAPH-treated erythrocytes	956:980	AAPH-treated erythrocytes	956:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	4	44	theme	galacturonic	510:521	arg1	acid					523:526	galacturonic acid	510:526	galacturonic acid	510:526	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	45	theme	AAPH-treated	956:967	arg1	erythrocytes					969:980	AAPH-treated erythrocytes	956:980	AAPH-treated erythrocytes	956:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	2	46	theme	monosaccharide	249:262	arg1	compositions					264:275	monosaccharide compositions	249:275	monosaccharide compositions	249:275	Their molecular weight, monosaccharide compositions and the ability to protect human erythrocytes from oxidative damage induced by AAPH were assessed.					
30177186	2	47	theme	oxidative	328:336	arg1	damage					338:343	oxidative damage	328:343	oxidative damage induced by AAPH	328:359	Their molecular weight, monosaccharide compositions and the ability to protect human erythrocytes from oxidative damage induced by AAPH were assessed.					
30177186	4	48	from	acid	693:696	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	1	49	theme	Saussurea	205:213	arg1	laniceps					215:222	Saussurea laniceps	205:222	Saussurea laniceps	205:222	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	0	50	from	damage	102:107	arg1	erythrocytes					118:129	human erythrocytes	112:129	human erythrocytes	112:129	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	1	51	theme	major	136:140	arg1	polysaccharides					142:156	Two major polysaccharides	132:156	Two major polysaccharides (SLT-3, SLT-4)	132:171	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	1	52	theme	laniceps	215:222	arg1	torus					196:200	torus	196:200	torus	196:200	Two major polysaccharides (SLT-3, SLT-4) were isolated from the torus of Saussurea laniceps.					
30177186	7	53	theme	laniceps	1192:1199	arg1	development					1174:1184	the development	1170:1184	the development of S. laniceps as a natural antioxidant	1170:1224	The results provide an important basis for the development of S. laniceps as a natural antioxidant.					
30177186	6	54	theme	intracellular	1014:1026	arg1	enzymes					1040:1046	intracellular antioxidant enzymes	1014:1046	intracellular antioxidant enzymes	1014:1046	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	54	theme	intracellular	1014:1026	arg1	CAT					1073:1075	CAT	1073:1075	CAT	1073:1075	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	54	theme	intracellular	1014:1026	arg1	GSH-Px					1062:1067	GSH-Px	1062:1067	GSH-Px	1062:1067	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	6	54	theme	intracellular	1014:1026	arg1	SOD					1057:1059	SOD	1057:1059	SOD	1057:1059	Furthermore, the activities of intracellular antioxidant enzymes, such as SOD, GSH-Px and CAT, were attenuated in polysaccharide treated cells.					
30177186	4	55	theme	molar	757:761	arg1	ratio					763:767	a molar ratio	755:767	a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01	755:806	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	5	56	from	GSSG	948:951	arg1	erythrocytes					969:980	AAPH-treated erythrocytes	956:980	AAPH-treated erythrocytes	956:980	Pretreatment with SLT-3 and SLT-4 reduced MDA content, inhibited the generation of intracellular ROS and maintained the balance of GSH and GSSG in AAPH-treated erythrocytes.					
30177186	4	57	theme	molar	576:580	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	0	58	theme	laniceps	54:61	arg1	torus					35:39	torus	35:39	torus	35:39	Two novel polysaccharides from the torus of Saussurea laniceps protect against AAPH-induced oxidative damage in human erythrocytes.					
30177186	2	59	theme	molecular	231:239	arg1	weight					241:246	Their molecular weight	225:246	Their molecular weight	225:246	Their molecular weight, monosaccharide compositions and the ability to protect human erythrocytes from oxidative damage induced by AAPH were assessed.					
30177186	4	60	from	galactose	538:546	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
30177186	4	61	from	rhamnose	672:679	arg1	ratio					582:586	a molar ratio	574:586	a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73	574:630	SLT-3 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.25:0.53:0.19:15.35:0.51:1.10:0.63:1.73, whereas SLT-4 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.92:5.61:0.93:19.50:2.42:5.27:3.01.					
29939724	3	0	from	structure	662:670	arg1	complex					711:717	complex	711:717	complex with chitobiose	711:733	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	3	1	theme	mutant	701:706	arg1	structure					662:670	the crystal structure	650:670	the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose	650:733	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	9	2	theme	decreasing	1564:1573	arg1	power					1586:1590	the decreasing hydrolytic power	1560:1590	the decreasing hydrolytic power	1560:1590	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	1	3	from	activity	181:188	arg1	hydrolases					203:212	glycoside hydrolases	193:212	glycoside hydrolases	193:212	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	2	4	theme	family	369:374	arg1	D					401:401	chitinase D	391:401	chitinase D from Serratia proteamaculans ( SpChiD)	391:440	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	4	theme	family	369:374	arg1	A					456:456	chitinase A	446:456	chitinase A from Serratia marcescens ( SmChiA)	446:491	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	4	theme	family	369:374	arg1	chitinases					379:388	two family 18 chitinases	365:388	two family 18 chitinases	365:388	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	7	5	with	Reactions	1217:1225	arg1	mutant					1241:1246	the best mutant	1232:1246	the best mutant	1232:1246	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	7	5	with	Reactions	1217:1225	arg1	SpChiD-D151N/Y226W/Y222A					1249:1272	SpChiD-D151N/Y226W/Y222A	1249:1272	SpChiD-D151N/Y226W/Y222A	1249:1272	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	4	6	theme	+2	937:938	arg1	subsite					940:946	the +2 subsite	933:946	the +2 subsite	933:946	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	2	7	theme	donor	601:605	arg1	sites					620:624	both donor and acceptor sites	596:624	both donor and acceptor sites	596:624	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	9	8	dep	powerful	1659:1666	arg1	general					1669:1675	general	1669:1675	general	1669:1675	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	1	9	theme	TG	177:178	arg1	activity					181:188	transglycosylation (TG) activity	157:188	transglycosylation (TG) activity in glycoside hydrolases	157:212	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	8	10	from	positions	1428:1436	arg1	SmChiA					1441:1446	SmChiA	1441:1446	SmChiA	1441:1446	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	8	10	from	positions	1428:1436	arg1	introduction					1380:1391	the introduction	1376:1391	the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A)	1376:1495	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	2	11	theme	important	519:527	arg1	residues					510:517	mutated residues	502:517	mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites	502:624	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	3	12	theme	inactive	679:686	arg1	mutant					701:706	the inactive SpChiD-E153Q mutant	675:706	the inactive SpChiD-E153Q mutant	675:706	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	7	13	theme	levels	1309:1314	arg1	accumulation					1288:1299	rapid accumulation	1282:1299	rapid accumulation of high levels of TG products that remained stable over time	1282:1360	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	6	14	theme	mutations	1163:1171	arg1	combination					1148:1158	The combination	1144:1158	The combination of mutations	1144:1171	The combination of mutations yielded even higher degrees of TG activity.					
29939724	9	15	theme	hydrolytic	1575:1584	arg1	power					1586:1590	the decreasing hydrolytic power	1560:1590	the decreasing hydrolytic power	1560:1590	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	1	16	theme	enzymes	270:276	arg1	construction					254:265	the construction	250:265	the construction of enzymes that can catalyze controlled synthesis of oligosaccharides	250:335	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	0	17	theme	Donor	90:94	arg1	Subsites					109:116	Donor and Acceptor Subsites	90:116	Donor and Acceptor Subsites	90:116	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	6	18	theme	activity	1207:1214	arg1	degrees					1193:1199	even higher degrees	1181:1199	even higher degrees of TG activity	1181:1214	The combination of mutations yielded even higher degrees of TG activity.					
29939724	2	19	theme	mutated	502:508	arg1	residues					510:517	mutated residues	502:517	mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites	502:624	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	6	20	theme	TG	1204:1205	arg1	activity					1207:1214	TG activity	1204:1214	TG activity	1204:1214	The combination of mutations yielded even higher degrees of TG activity.					
29939724	9	21	theme	glycoside	1744:1752	arg1	hydrolases					1754:1763	retaining glycoside hydrolases	1734:1763	retaining glycoside hydrolases	1734:1763	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	5	22	theme	DXDXE	1031:1035	arg1	motif					1037:1041	the catalytically important DXDXE motif	1003:1041	the catalytically important DXDXE motif	1003:1041	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	0	23	theme	Acceptor	100:107	arg1	Subsites					109:116	Donor and Acceptor Subsites	90:116	Donor and Acceptor Subsites	90:116	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	5	24	theme	hydrolytic	1066:1075	arg1	activity					1077:1084	hydrolytic activity	1066:1084	hydrolytic activity	1066:1084	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	2	25	theme	chitinase	446:454	arg1	A					456:456	chitinase A	446:456	chitinase A from Serratia marcescens ( SmChiA)	446:491	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	25	theme	chitinase	446:454	arg1	chitinases					379:388	two family 18 chitinases	365:388	two family 18 chitinases	365:388	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	0	26	from	Activity	42:49	arg1	Chitinases					64:73	Family 18 Chitinases	54:73	Family 18 Chitinases	54:73	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	5	27	theme	apparent	1122:1129	arg1	activity					1134:1141	apparent TG activity	1122:1141	apparent TG activity	1122:1141	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	9	28	theme	intermediate	1628:1639	arg1	states					1641:1646	intermediate states	1628:1646	intermediate states	1628:1646	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	8	29	theme	TG	1520:1521	arg1	efficiency					1523:1532	TG efficiency	1520:1532	TG efficiency	1520:1532	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	2	30	theme	chitinase	391:399	arg1	D					401:401	chitinase D	391:401	chitinase D from Serratia proteamaculans ( SpChiD)	391:440	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	30	theme	chitinase	391:399	arg1	chitinases					379:388	two family 18 chitinases	365:388	two family 18 chitinases	365:388	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	0	31	theme	Key	0:2	arg1	Residues					4:11	Key Residues	0:11	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases	0:73	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	9	32	theme	stability	1615:1623	arg1	combination					1545:1555	the combination	1541:1555	the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states	1541:1646	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	2	33	theme	acceptor	611:618	arg1	sites					620:624	both donor and acceptor sites	596:624	both donor and acceptor sites	596:624	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	0	34	dep	Insights	76:83	arg1	Residues					4:11	Key Residues	0:11	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases	0:73	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	1	35	theme	glycoside	193:201	arg1	hydrolases					203:212	glycoside hydrolases	193:212	glycoside hydrolases	193:212	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	0	36	theme	Transglycosylation	23:40	arg1	Activity					42:49	Transglycosylation Activity	23:49	Transglycosylation Activity in Family 18 Chitinases	23:73	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	4	37	with	mutations	771:779	arg1	effect					799:804	a beneficial effect	786:804	a beneficial effect on TG activity	786:819	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	2	38	theme	reaction	549:556	arg1	intermediate					558:569	the reaction intermediate	545:569	the reaction intermediate	545:569	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	9	39	theme	retaining	1734:1742	arg1	hydrolases					1754:1763	retaining glycoside hydrolases	1734:1763	retaining glycoside hydrolases	1734:1763	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	4	40	from	effect	799:804	arg1	activity					812:819	TG activity	809:819	TG activity	809:819	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	1	41	theme	controlled	296:305	arg1	synthesis					307:315	controlled synthesis	296:315	controlled synthesis of oligosaccharides	296:335	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	9	42	theme	power	1586:1590	arg1	combination					1545:1555	the combination	1541:1555	the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states	1541:1646	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	7	43	theme	best	1236:1239	arg1	mutant					1241:1246	the best mutant	1232:1246	the best mutant	1232:1246	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	7	43	theme	best	1236:1239	arg1	SpChiD-D151N/Y226W/Y222A					1249:1272	SpChiD-D151N/Y226W/Y222A	1249:1272	SpChiD-D151N/Y226W/Y222A	1249:1272	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	5	44	theme	TG	1131:1132	arg1	activity					1134:1141	apparent TG activity	1122:1141	apparent TG activity	1122:1141	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	9	45	theme	subsite	1593:1599	arg1	affinity					1601:1608	subsite affinity	1593:1608	subsite affinity	1593:1608	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	7	46	theme	TG	1319:1320	arg1	products					1322:1329	TG products	1319:1329	TG products that remained stable over time	1319:1360	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	8	47	dep	SmChiA	1441:1446	arg1	Y390F					1473:1477	Y390F	1473:1477	Y390F	1473:1477	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	8	47	dep	SmChiA	1441:1446	arg1	Y163A					1449:1453	Y163A	1449:1453	Y163A	1449:1453	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	5	48	theme	important	1021:1029	arg1	motif					1037:1041	the catalytically important DXDXE motif	1003:1041	the catalytically important DXDXE motif	1003:1041	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	4	49	theme	TG	809:810	arg1	activity					812:819	TG activity	809:819	TG activity	809:819	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	8	50	contain	had	1497:1499	arg2	effects					1509:1515	similar effects	1501:1515	similar effects	1501:1515	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	8	50	contain	had	1497:1499	arg1	introduction					1380:1391	the introduction	1376:1391	the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A)	1376:1495	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	2	51	theme	Serratia	408:415	arg1	proteamaculans					417:430	Serratia proteamaculans	408:430	Serratia proteamaculans ( SpChiD)	408:440	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	51	theme	Serratia	408:415	arg1	SpChiD					434:439	SpChiD	434:439	SpChiD	434:439	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	1	52	theme	oligosaccharides	320:335	arg1	synthesis					307:315	controlled synthesis	296:315	controlled synthesis of oligosaccharides	296:335	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	3	53	theme	mutant	635:640	arg1	design					642:647	mutant design	635:647	mutant design	635:647	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	5	54	theme	D151	975:978	arg1	exchange					963:970	exchange	963:970	exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine	963:1056	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	9	55	theme	states	1641:1646	arg1	power					1586:1590	the decreasing hydrolytic power	1560:1590	the decreasing hydrolytic power	1560:1590	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	9	55	theme	states	1641:1646	arg1	affinity					1601:1608	subsite affinity	1593:1608	subsite affinity	1593:1608	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	9	55	theme	states	1641:1646	arg1	stability					1615:1623	stability	1615:1623	stability	1615:1623	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	0	56	theme	Family	54:59	arg1	Chitinases					64:73	Family 18 Chitinases	54:73	Family 18 Chitinases	54:73	Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.					
29939724	8	57	theme	similar	1501:1507	arg1	effects					1509:1515	similar effects	1501:1515	similar effects	1501:1515	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	2	58	theme	substrate	575:583	arg1	binding					585:591	substrate binding	575:591	substrate binding	575:591	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	8	59	theme	mutations	1406:1414	arg1	introduction					1380:1391	the introduction	1376:1391	the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A)	1376:1495	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	9	60	theme	hypertransglycosylating	1699:1721	arg1	mutants					1723:1729	hypertransglycosylating mutants	1699:1729	hypertransglycosylating mutants of retaining glycoside hydrolases	1699:1763	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	8	61	theme	analogous	1396:1404	arg1	mutations					1406:1414	analogous mutations	1396:1414	analogous mutations	1396:1414	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	5	62	theme	concomitant	1098:1108	arg1	increase					1110:1117	a concomitant increase	1096:1117	a concomitant increase in apparent TG activity	1096:1141	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	2	63	from	proteamaculans	417:430	arg1	D					401:401	chitinase D	391:401	chitinase D from Serratia proteamaculans ( SpChiD)	391:440	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	63	from	proteamaculans	417:430	arg1	A					456:456	chitinase A	446:456	chitinase A from Serratia marcescens ( SmChiA)	446:491	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	63	from	proteamaculans	417:430	arg1	chitinases					379:388	two family 18 chitinases	365:388	two family 18 chitinases	365:388	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	5	64	theme	middle	985:990	arg1	residue					992:998	the middle residue	981:998	the middle residue in the catalytically important DXDXE motif	981:1041	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	5	64	theme	middle	985:990	arg1	D151					975:978	D151	975:978	D151	975:978	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	4	65	theme	beneficial	788:797	arg1	effect					799:804	a beneficial effect	786:804	a beneficial effect on TG activity	786:819	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	5	66	from	increase	1110:1117	arg1	activity					1134:1141	apparent TG activity	1122:1141	apparent TG activity	1122:1141	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	2	67	theme	TG	350:351	arg1	activity					353:360	TG activity	350:360	TG activity	350:360	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	3	68	with	complex	711:717	arg1	chitobiose					724:733	chitobiose	724:733	chitobiose	724:733	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	8	69	theme	same	1423:1426	arg1	positions					1428:1436	the same positions	1419:1436	the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A)	1419:1495	Importantly, the introduction of analogous mutations at the same positions in SmChiA (Y163A equal to Y28A and Y390F similar to Y222A) had similar effects on TG efficiency.					
29939724	7	70	theme	high	1304:1307	arg1	levels					1309:1314	high levels	1304:1314	high levels of TG products that remained stable over time	1304:1360	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	2	71	contain	have	497:500	arg1	we					494:495	we	494:495	we	494:495	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	71	contain	have	497:500	arg2	residues					510:517	mutated residues	502:517	mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites	502:624	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	4	72	dep	mutations	771:779	arg1	Y226W					916:920	Y226W	916:920	Y226W	916:920	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	4	72	dep	mutations	771:779	arg1	Y222A					876:880	Y222A	876:880	Y222A	876:880	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	4	72	dep	mutations	771:779	arg1	mutations					771:779	three mutations	765:779	three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite)	765:947	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	4	72	dep	mutations	771:779	arg1	Y28A					822:825	Y28A	822:825	Y28A	822:825	We identified three mutations with a beneficial effect on TG activity: Y28A (affecting the -1 subsite and the intermediate), Y222A (affecting the intermediate), and Y226W (affecting the +2 subsite).					
29939724	9	73	theme	hydrolases	1754:1763	arg1	mutants					1723:1729	hypertransglycosylating mutants	1699:1729	hypertransglycosylating mutants of retaining glycoside hydrolases	1699:1763	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	5	74	from	residue	992:998	arg1	motif					1037:1041	the catalytically important DXDXE motif	1003:1041	the catalytically important DXDXE motif	1003:1041	Furthermore, exchange of D151, the middle residue in the catalytically important DXDXE motif, to asparagine reduced hydrolytic activity ≤99% with a concomitant increase in apparent TG activity.					
29939724	3	75	theme	crystal	654:660	arg1	structure					662:670	the crystal structure	650:670	the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose	650:733	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	7	76	theme	rapid	1282:1286	arg1	accumulation					1288:1299	rapid accumulation	1282:1299	rapid accumulation of high levels of TG products that remained stable over time	1282:1360	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29939724	2	77	from	marcescens	472:481	arg1	D					401:401	chitinase D	391:401	chitinase D from Serratia proteamaculans ( SpChiD)	391:440	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	77	from	marcescens	472:481	arg1	A					456:456	chitinase A	446:456	chitinase A from Serratia marcescens ( SmChiA)	446:491	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	2	77	from	marcescens	472:481	arg1	chitinases					379:388	two family 18 chitinases	365:388	two family 18 chitinases	365:388	To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for stabilizing the reaction intermediate and substrate binding in both donor and acceptor sites.					
29939724	9	78	theme	affinity	1601:1608	arg1	combination					1545:1555	the combination	1541:1555	the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states	1541:1646	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	9	79	theme	powerful	1659:1666	arg1	strategy					1677:1684	a powerful, general strategy	1657:1684	a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases	1657:1763	Thus, the combination of the decreasing hydrolytic power, subsite affinity, and stability of intermediate states provides a powerful, general strategy for creating hypertransglycosylating mutants of retaining glycoside hydrolases.					
29939724	3	80	theme	SpChiD-E153Q	688:699	arg1	mutant					701:706	the inactive SpChiD-E153Q mutant	675:706	the inactive SpChiD-E153Q mutant	675:706	To help mutant design, the crystal structure of the inactive SpChiD-E153Q mutant in complex with chitobiose was determined.					
29939724	6	81	theme	higher	1186:1191	arg1	degrees					1193:1199	even higher degrees	1181:1199	even higher degrees of TG activity	1181:1214	The combination of mutations yielded even higher degrees of TG activity.					
29939724	1	82	theme	transglycosylation	157:174	arg1	activity					181:188	transglycosylation (TG) activity	157:188	transglycosylation (TG) activity in glycoside hydrolases	157:212	Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides.					
29939724	7	83	theme	products	1322:1329	arg1	levels					1309:1314	high levels	1304:1314	high levels of TG products that remained stable over time	1304:1360	Reactions with the best mutant, SpChiD-D151N/Y226W/Y222A, led to rapid accumulation of high levels of TG products that remained stable over time.					
29775057	2	0	theme	economical	318:327	arg1	option					356:361	an economical and environmental friendly option	315:361	an economical and environmental friendly option	315:361	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	2	1	contain	has	226:228	arg2	value					247:251	a high nutritive value	230:251	a high nutritive value	230:251	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	2	1	contain	has	226:228	arg1	okara					220:224	okara	220:224	okara	220:224	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	8	2	theme	potential	1064:1072	arg1	food					1085:1088	a potential functional food	1062:1088	a potential functional food for animal feed	1062:1104	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	8	2	theme	potential	1064:1072	arg1	okara					1053:1057	fermented okara	1043:1057	fermented okara	1043:1057	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	4	3	theme	biofermenting	509:521	arg1	okara					523:527	biofermenting okara	509:527	biofermenting okara	509:527	The study revealed that biofermenting okara improves its nutritional composition.					
29775057	0	4	theme	Probiotic	88:96	arg1	Fermentation					110:121	Probiotic Solid-State Fermentation	88:121	Probiotic Solid-State Fermentation	88:121	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	8	5	theme	entire	994:999	arg1	work					1001:1004	this entire work	989:1004	this entire work	989:1004	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	5	6	theme	DPPH	662:665	arg1	test					667:670	DPPH test	662:670	DPPH test	662:670	It was found that the metabolomic composition (by GC-MS analysis) and antioxidant activity (by DPPH test) improved after the microbial fermentations.					
29775057	7	7	theme	respective	839:848	arg1	pathways					863:870	their respective biosynthesis pathways	833:870	their respective biosynthesis pathways	833:870	Further, the metabolites were traced back to their respective biosynthesis pathways, in order to understand the biochemical reactions being triggered during the fermentation processes.					
29775057	5	8	theme	GC-MS	617:621	arg1	analysis					623:630	GC-MS analysis	617:630	GC-MS analysis	617:630	It was found that the metabolomic composition (by GC-MS analysis) and antioxidant activity (by DPPH test) improved after the microbial fermentations.					
29775057	7	9	theme	biosynthesis	850:861	arg1	pathways					863:870	their respective biosynthesis pathways	833:870	their respective biosynthesis pathways	833:870	Further, the metabolites were traced back to their respective biosynthesis pathways, in order to understand the biochemical reactions being triggered during the fermentation processes.					
29775057	3	10	theme	probiotic	422:430	arg1	cultures					456:463	the probiotic FDA-approved food-grade cultures	418:463	the probiotic FDA-approved food-grade cultures used in this study	418:482	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	10	theme	probiotic	422:430	arg1	oligosporus					373:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	10	theme	probiotic	422:430	arg1	plantarum					403:411	Lactobacillus plantarum	389:411	Lactobacillus plantarum	389:411	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	0	11	theme	Solid-State	98:108	arg1	Fermentation					110:121	Probiotic Solid-State Fermentation	88:121	Probiotic Solid-State Fermentation	88:121	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	8	12	theme	fermented	1043:1051	arg1	food					1085:1088	a potential functional food	1062:1088	a potential functional food for animal feed	1062:1104	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	8	12	theme	fermented	1043:1051	arg1	okara					1053:1057	fermented okara	1043:1057	fermented okara	1043:1057	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	8	13	theme	work	1001:1004	arg1	findings					977:984	The findings	973:984	The findings of this entire work	973:1004	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	3	14	theme	FDA-approved	432:443	arg1	cultures					456:463	the probiotic FDA-approved food-grade cultures	418:463	the probiotic FDA-approved food-grade cultures used in this study	418:482	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	14	theme	FDA-approved	432:443	arg1	oligosporus					373:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	14	theme	FDA-approved	432:443	arg1	plantarum					403:411	Lactobacillus plantarum	389:411	Lactobacillus plantarum	389:411	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	6	15	theme	better	772:777	arg1	results					779:785	better results	772:785	better results	772:785	Of the two, okara fermented with R. oligosporus showed better results.					
29775057	3	16	theme	Rhizopus	364:371	arg1	cultures					456:463	the probiotic FDA-approved food-grade cultures	418:463	the probiotic FDA-approved food-grade cultures used in this study	418:482	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	16	theme	Rhizopus	364:371	arg1	oligosporus					373:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	16	theme	Rhizopus	364:371	arg1	plantarum					403:411	Lactobacillus plantarum	389:411	Lactobacillus plantarum	389:411	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	5	17	theme	antioxidant	637:647	arg1	activity					649:656	antioxidant activity	637:656	antioxidant activity (by DPPH test)	637:671	It was found that the metabolomic composition (by GC-MS analysis) and antioxidant activity (by DPPH test) improved after the microbial fermentations.					
29775057	2	18	theme	friendly	347:354	arg1	option					356:361	an economical and environmental friendly option	315:361	an economical and environmental friendly option	315:361	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	0	19	theme	Nutritional	21:31	arg1	Composition					33:43	Improved Nutritional Composition	12:43	Improved Nutritional Composition of Potential Functional Food (Okara)	12:80	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	8	20	theme	functional	1074:1083	arg1	food					1085:1088	a potential functional food	1062:1088	a potential functional food for animal feed	1062:1104	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	8	20	theme	functional	1074:1083	arg1	okara					1053:1057	fermented okara	1043:1057	fermented okara	1043:1057	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	5	21	theme	metabolomic	589:599	arg1	composition					601:611	the metabolomic composition	585:611	the metabolomic composition (by GC-MS analysis)	585:631	It was found that the metabolomic composition (by GC-MS analysis) and antioxidant activity (by DPPH test) improved after the microbial fermentations.					
29775057	0	22	theme	Improved	12:19	arg1	Composition					33:43	Improved Nutritional Composition	12:43	Improved Nutritional Composition of Potential Functional Food (Okara)	12:80	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	5	23	theme	microbial	692:700	arg1	fermentations					702:714	the microbial fermentations	688:714	the microbial fermentations	688:714	It was found that the metabolomic composition (by GC-MS analysis) and antioxidant activity (by DPPH test) improved after the microbial fermentations.					
29775057	7	24	theme	fermentation	949:960	arg1	processes					962:970	the fermentation processes	945:970	the fermentation processes	945:970	Further, the metabolites were traced back to their respective biosynthesis pathways, in order to understand the biochemical reactions being triggered during the fermentation processes.					
29775057	1	25	theme	soymilk	187:193	arg1	industry					204:211	the soymilk and tofu industry	183:211	industry	204:211	Okara is a major agro-waste, generated as a byproduct from the soymilk and tofu industry.					
29775057	3	26	theme	food-grade	445:454	arg1	cultures					456:463	the probiotic FDA-approved food-grade cultures	418:463	the probiotic FDA-approved food-grade cultures used in this study	418:482	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	26	theme	food-grade	445:454	arg1	oligosporus					373:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus	364:383	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	3	26	theme	food-grade	445:454	arg1	plantarum					403:411	Lactobacillus plantarum	389:411	Lactobacillus plantarum	389:411	Rhizopus oligosporus and Lactobacillus plantarum were the probiotic FDA-approved food-grade cultures used in this study.					
29775057	0	27	theme	Composition	33:43	arg1	Analysis					0:7	Analysis	0:7	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.	0:122	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	6	28	theme	R.	750:751	arg1	oligosporus					753:763	R. oligosporus	750:763	R. oligosporus	750:763	Of the two, okara fermented with R. oligosporus showed better results.					
29775057	1	29	theme	tofu	199:202	arg1	industry					204:211	the soymilk and tofu industry	183:211	industry	204:211	Okara is a major agro-waste, generated as a byproduct from the soymilk and tofu industry.					
29775057	0	30	theme	Functional	58:67	arg1	Okara					75:79	Okara	75:79	Okara	75:79	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	0	30	theme	Functional	58:67	arg1	Food					69:72	Potential Functional Food	48:72	Potential Functional Food (Okara)	48:80	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	0	31	theme	Potential	48:56	arg1	Okara					75:79	Okara	75:79	Okara	75:79	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	0	31	theme	Potential	48:56	arg1	Food					69:72	Potential Functional Food	48:72	Potential Functional Food (Okara)	48:80	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
29775057	4	32	theme	nutritional	542:552	arg1	composition					554:564	its nutritional composition	538:564	its nutritional composition	538:564	The study revealed that biofermenting okara improves its nutritional composition.					
29775057	8	33	theme	animal	1094:1099	arg1	feed					1101:1104	animal feed	1094:1104	animal feed	1094:1104	The findings of this entire work open up the possibility of employing fermented okara as a potential functional food for animal feed.					
29775057	1	34	from	industry	204:211	arg1	byproduct					168:176	a byproduct	166:176	a byproduct from the soymilk and tofu industry	166:211	Okara is a major agro-waste, generated as a byproduct from the soymilk and tofu industry.					
29775057	1	35	theme	major	135:139	arg1	agro-waste					141:150	a major agro-waste	133:150	a major agro-waste	133:150	Okara is a major agro-waste, generated as a byproduct from the soymilk and tofu industry.					
29775057	1	35	theme	major	135:139	arg1	Okara					124:128	Okara	124:128	Okara	124:128	Okara is a major agro-waste, generated as a byproduct from the soymilk and tofu industry.					
29775057	2	36	theme	nutritive	237:245	arg1	value					247:251	a high nutritive value	230:251	a high nutritive value	230:251	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	2	37	theme	state	290:294	arg1	biofermentation					296:310	solid state biofermentation	284:310	solid state biofermentation	284:310	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	7	38	theme	biochemical	900:910	arg1	reactions					912:920	the biochemical reactions	896:920	the biochemical reactions being triggered during the fermentation processes	896:970	Further, the metabolites were traced back to their respective biosynthesis pathways, in order to understand the biochemical reactions being triggered during the fermentation processes.					
29775057	2	39	theme	high	232:235	arg1	value					247:251	a high nutritive value	230:251	a high nutritive value	230:251	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	2	40	theme	solid	284:288	arg1	biofermentation					296:310	solid state biofermentation	284:310	solid state biofermentation	284:310	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	2	41	theme	environmental	333:345	arg1	option					356:361	an economical and environmental friendly option	315:361	an economical and environmental friendly option	315:361	Since okara has a high nutritive value, reusing it as a substrate for solid state biofermentation is an economical and environmental friendly option.					
29775057	0	42	theme	Food	69:72	arg1	Composition					33:43	Improved Nutritional Composition	12:43	Improved Nutritional Composition of Potential Functional Food (Okara)	12:80	Analysis of Improved Nutritional Composition of Potential Functional Food (Okara) after Probiotic Solid-State Fermentation.					
30605786	3	0	theme	major	466:470	arg1	factor					482:487	a major virulence factor	464:487	a major virulence factor for encapsulated pathogens	464:514	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	8	1	theme	bacterial	1355:1363	arg1	component					1365:1373	this bacterial component	1350:1373	this bacterial component	1350:1373	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	5	2	dep	Streptococcus	867:879	arg1	pneumoniae					881:890	pneumoniae	881:890	pneumoniae	881:890	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	3	3	dep	S.	355:356	arg1	suis					358:361	suis	358:361	suis	358:361	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	4	theme	virulence	472:480	arg1	factor					482:487	a major virulence factor	464:487	a major virulence factor for encapsulated pathogens	464:514	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	1	5	theme	S.	239:240	arg1	serotypes					247:255	the top six S. suis serotypes	227:255	the top six S. suis serotypes causing clinical disease in pigs	227:288	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
30605786	4	6	theme	β1-4	800:803	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	6	theme	β1-4	800:803	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	3	7	theme	first	544:548	arg1	time					550:553	the first time	540:553	the first time	540:553	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	4	8	theme	α1-P-2	709:714	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	8	theme	α1-P-2	709:714	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	5	9	with	reaction	940:947	arg1	reference					980:988	reference anti-S	980:995	reference anti-S	980:995	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	1	10	theme	suis	242:245	arg1	serotypes					247:255	the top six S. suis serotypes	227:255	the top six S. suis serotypes causing clinical disease in pigs	227:288	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
30605786	0	11	theme	assignment	93:102	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.	0:170	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	5	12	theme	dot-blot	906:913	arg1	analyses					915:922	dot-blot analyses	906:922	dot-blot analyses	906:922	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	6	13	dep	serum	1021:1025	arg1	serotype					1003:1010	suis serotype 8	998:1012	suis serotype 8	998:1012	suis serotype 8 rabbit serum.					
30605786	6	14	theme	suis	998:1001	arg1	serotype					1003:1010	suis serotype 8	998:1012	suis serotype 8	998:1012	suis serotype 8 rabbit serum.					
30605786	4	15	theme	unit	684:687	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	15	theme	unit	684:687	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	15	theme	unit	684:687	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	16	theme	α1-	750:752	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	16	theme	α1-	750:752	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	3	17	theme	encapsulated	493:504	arg1	pathogens					506:514	encapsulated pathogens	493:514	encapsulated pathogens	493:514	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	18	theme	capsular	395:402	arg1	CPS					420:422	CPS	420:422	CPS	420:422	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	18	theme	capsular	395:402	arg1	polysaccharide					404:417	capsular polysaccharide	395:417	capsular polysaccharide (CPS) antigenicity	395:436	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	7	19	theme	putative	1130:1137	arg1	glycosyltransferases					1139:1158	putative glycosyltransferases	1130:1158	putative glycosyltransferases	1130:1158	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	0	20	theme	functions	107:115	arg1	serotypes					46:54	Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis	27:169	serotypes	46:54	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	0	20	theme	functions	107:115	arg1	assignment					93:102	assignment	93:102	assignment of functions of the cps locus genes involved in their biosynthesis	93:169	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	8	21	theme	composition	1292:1302	arg1	Knowledge					1261:1269	Knowledge	1261:1269	Knowledge of CPS structure and composition	1261:1302	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	0	22	theme	Structure	0:8	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.	0:170	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	1	23	theme	Streptococcus	172:184	arg1	serotypes					191:199	Streptococcus suis serotypes 7 and 8	172:207	Streptococcus suis serotypes 7 and 8	172:207	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
30605786	8	24	theme	component	1365:1373	arg1	role					1342:1345	the role	1338:1345	the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8	1338:1444	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	4	25	dep	sequences	689:697	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	25	dep	sequences	689:697	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	25	dep	sequences	689:697	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	1	26	theme	suis	186:189	arg1	serotypes					191:199	Streptococcus suis serotypes 7 and 8	172:207	Streptococcus suis serotypes 7 and 8	172:207	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
30605786	3	27	theme	serotyping	363:372	arg1	system					374:379	S. suis serotyping system	355:379	S. suis serotyping system	355:379	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	5	28	theme	anti-S	990:995	arg1	reference					980:988	reference anti-S	980:995	reference anti-S	980:995	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	7	29	dep	serotypes	1058:1066	arg1	sequences					1076:1084	sequences	1076:1084	sequences	1076:1084	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	7	30	theme	repeating	1216:1224	arg1	units					1226:1230	the repeating units	1212:1230	the repeating units	1212:1230	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	3	31	theme	polysaccharide	404:417	arg1	antigenicity					425:436	capsular polysaccharide (CPS) antigenicity	395:436	capsular polysaccharide (CPS) antigenicity	395:436	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	0	32	theme	Streptococcus	27:39	arg1	serotypes					46:54	Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis	27:169	serotypes	46:54	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	1	33	theme	clinical	265:272	arg1	disease					274:280	clinical disease	265:280	clinical disease	265:280	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
30605786	0	34	theme	cps	124:126	arg1	genes					134:138	the cps locus genes	120:138	the cps locus genes involved in their biosynthesis	120:169	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	8	35	theme	disease	1402:1408	arg1	pathogenesis					1382:1393	the pathogenesis	1378:1393	the pathogenesis of the disease caused by S. suis serotypes 7 and 8	1378:1444	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	4	36	theme	D-FucNAc4N	739:748	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	36	theme	D-FucNAc4N	739:748	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	3	37	theme	S.	355:356	arg1	system					374:379	S. suis serotyping system	355:379	S. suis serotyping system	355:379	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	4	38	theme	α1-P-4	784:789	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	38	theme	α1-P-4	784:789	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	5	39	theme	type	892:895	arg1	CPS					901:903	Streptococcus pneumoniae type 19F CPS	867:903	Streptococcus pneumoniae type 19F CPS	867:903	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	7	40	theme	loci	1116:1119	arg1	serotypes					1058:1066	S. suis serotypes 7 and 8 sequences and genes	1050:1094	serotypes	1058:1066	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	7	40	theme	loci	1116:1119	arg1	genes					1090:1094	genes	1090:1094	genes	1090:1094	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	6	41	theme	rabbit	1014:1019	arg1	serum					1021:1025	rabbit serum	1014:1025	rabbit serum	1014:1025	suis serotype 8 rabbit serum.					
30605786	0	42	theme	serotypes	46:54	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.	0:170	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	0	43	theme	genes	134:138	arg1	functions					107:115	functions	107:115	functions of the cps locus genes involved in their biosynthesis	107:169	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	5	44	theme	19F	897:899	arg1	CPS					901:903	Streptococcus pneumoniae type 19F CPS	867:903	Streptococcus pneumoniae type 19F CPS	867:903	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	5	45	theme	serotype	836:843	arg1	CPS					847:849	serotype 8 CPS	836:849	serotype 8 CPS	836:849	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	4	46	theme	Chemical	623:630	arg1	data					650:653	Chemical and spectroscopic data	623:653	Chemical and spectroscopic data	623:653	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	0	47	theme	suis	41:44	arg1	serotypes					46:54	Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis	27:169	serotypes	46:54	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	5	48	theme	19F	956:958	arg1	polysaccharide					960:973	the 19F polysaccharide	952:973	the 19F polysaccharide	952:973	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	0	49	theme	locus	128:132	arg1	genes					134:138	the cps locus genes	120:138	the cps locus genes involved in their biosynthesis	120:169	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	3	50	theme	serotypes	599:607	arg1	structures					585:594	structures	585:594	structures	585:594	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	50	theme	serotypes	599:607	arg1	compositions					568:579	the chemical compositions	555:579	the chemical compositions	555:579	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	51	theme	chemical	559:566	arg1	compositions					568:579	the chemical compositions	555:579	the chemical compositions	555:579	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	8	52	theme	structure	1278:1286	arg1	Knowledge					1261:1269	Knowledge	1261:1269	Knowledge of CPS structure and composition	1261:1302	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	4	53	theme	D-Glc	805:809	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	53	theme	D-Glc	805:809	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	5	54	theme	polysaccharide	960:973	arg1	reaction					940:947	a strong reaction	931:947	a strong reaction of the 19F polysaccharide with reference anti-S	931:995	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	7	55	theme	S.	1050:1051	arg1	serotypes					1058:1066	S. suis serotypes 7 and 8 sequences and genes	1050:1094	serotypes	1058:1066	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	0	56	dep	serotypes	46:54	arg1	polysaccharides					73:87	7 and 8 capsular polysaccharides	56:87	7 and 8 capsular polysaccharides	56:87	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	8	57	from	role	1342:1345	arg1	pathogenesis					1382:1393	the pathogenesis	1378:1393	the pathogenesis of the disease caused by S. suis serotypes 7 and 8	1378:1444	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	4	58	theme	repeating	674:682	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	58	theme	repeating	674:682	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	58	theme	repeating	674:682	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	59	theme	D-ManNAc	791:798	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	59	theme	D-ManNAc	791:798	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	8	60	theme	S.	1420:1421	arg1	serotypes					1428:1436	S. suis serotypes 7 and 8	1420:1444	serotypes	1428:1436	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	4	61	theme	[2	775:776	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	61	theme	[2	775:776	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	7	62	theme	units	1226:1230	arg1	biosynthesis					1196:1207	the biosynthesis	1192:1207	the biosynthesis of the repeating units	1192:1230	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	4	63	theme	following	664:672	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	63	theme	following	664:672	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	63	theme	following	664:672	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	8	64	theme	CPS	1274:1276	arg1	structure					1278:1286	CPS structure	1274:1286	CPS structure	1274:1286	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	7	65	theme	responsible	1176:1186	arg1	glycosyltransferases					1139:1158	putative glycosyltransferases	1130:1158	putative glycosyltransferases	1130:1158	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	4	66	theme	α1-4	722:725	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	66	theme	α1-4	722:725	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	67	theme	L-Rha	703:707	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	67	theme	L-Rha	703:707	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	3	68	dep	serotypes	599:607	arg1	7					609:609	7	609:609	7	609:609	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	3	68	dep	serotypes	599:607	arg1	CPSs					617:620	8 CPSs	615:620	8 CPSs	615:620	Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs.					
30605786	4	69	theme	L-Rha	778:782	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	69	theme	L-Rha	778:782	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	5	70	theme	Streptococcus	867:879	arg1	CPS					901:903	Streptococcus pneumoniae type 19F CPS	867:903	Streptococcus pneumoniae type 19F CPS	867:903	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	4	71	theme	D-Gal	716:720	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	71	theme	D-Gal	716:720	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	2	72	theme	limited	296:302	arg1	information					304:314	limited information	296:314	limited information	296:314	Yet, limited information is available on these serotypes.					
30605786	8	73	theme	suis	1423:1426	arg1	serotypes					1428:1436	S. suis serotypes 7 and 8	1420:1444	serotypes	1428:1436	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.					
30605786	0	74	theme	capsular	64:71	arg1	polysaccharides					73:87	7 and 8 capsular polysaccharides	56:87	7 and 8 capsular polysaccharides	56:87	Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis.					
30605786	4	75	theme	D-GlcA	727:732	arg1	n					754:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n	700:754	[3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7	700:769	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	75	theme	D-GlcA	727:732	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	76	theme	α1-	811:813	arg1	n					815:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	[2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n	775:815	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	4	76	theme	α1-	811:813	arg1	sequences					689:697	the following repeating unit sequences	660:697	the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8	660:830	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	5	77	theme	strong	933:938	arg1	reaction					940:947	a strong reaction	931:947	a strong reaction of the 19F polysaccharide with reference anti-S	931:995	As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S.					
30605786	4	78	theme	spectroscopic	636:648	arg1	data					650:653	Chemical and spectroscopic data	623:653	Chemical and spectroscopic data	623:653	Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8.					
30605786	7	79	theme	suis	1053:1056	arg1	serotypes					1058:1066	S. suis serotypes 7 and 8 sequences and genes	1050:1094	serotypes	1058:1066	A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established.					
30605786	1	80	theme	top	231:233	arg1	serotypes					247:255	the top six S. suis serotypes	227:255	the top six S. suis serotypes causing clinical disease in pigs	227:288	Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs.					
31412567	3	0	theme	0.18	890:893	arg1	yield					845:849	the polysaccharide yield	826:849	the polysaccharide yield from TCBL obtained by EUCE	826:876	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	3	0	theme	0.18	890:893	arg1	%					894:894	4.78% ± 0.18%	882:894	4.78% ± 0.18%	882:894	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	2	1	theme	enzyme	756:761	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	6	2	theme	antitumor	1133:1141	arg1	activity					1143:1150	antitumor activity	1133:1150	antitumor activity	1133:1150	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	7	3	theme	IC50	1335:1338	arg1	value					1340:1344	its IC50 value	1331:1344	its IC50 value	1331:1344	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	2	4	theme	technology	587:596	arg1	parameters					598:607	Optimal technology parameters	579:607	Optimal technology parameters	579:607	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	4	theme	technology	587:596	arg1	follows					628:634	follows	628:634	follows	628:634	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	7	5	theme	inhibitory	1284:1293	arg1	capacity					1295:1302	the highest inhibitory capacity	1272:1302	the highest inhibitory capacity	1272:1302	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	8	6	theme	µg·mL-1	1476:1482	arg1	values					1450:1455	IC50 values	1445:1455	IC50 values of 169.0 and 132.0 µg·mL-1	1445:1482	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	0	7	theme	excellent	155:163	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	7	theme	excellent	155:163	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	7	theme	excellent	155:163	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	7	theme	excellent	155:163	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	11	8	theme	cancer	1840:1845	arg1	activity					1847:1854	human cervical cancer activity	1825:1854	human cervical cancer activity	1825:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	9	9	theme	highest	1497:1503	arg1	effect					1515:1520	the highest antitumor effect	1493:1520	the highest antitumor effect	1493:1520	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	1	10	theme	optimum	432:438	arg1	technology					451:460	the optimum extraction technology	428:460	the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme	428:519	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	11	11	theme	human	1825:1829	arg1	cancer					1840:1845	human cervical cancer	1825:1845	human cervical cancer activity	1825:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	0	12	theme	bioactive	183:191	arg1	polysaccharides					193:207	bioactive polysaccharides	183:207	bioactive polysaccharides	183:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	9	13	theme	human	1525:1529	arg1	Hela					1554:1557	Hela	1554:1557	Hela	1554:1557	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	9	13	theme	human	1525:1529	arg1	cells					1547:1551	human cervical cancer cells	1525:1551	human cervical cancer cells (Hela)	1525:1558	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	0	14	theme	S.	115:116	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	15	theme	Taxus	368:372	arg1	branches					384:391	Taxus cuspidata branches	368:391	Taxus cuspidata branches	368:391	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	9	16	theme	cancer	1540:1545	arg1	Hela					1554:1557	Hela	1554:1557	Hela	1554:1557	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	9	16	theme	cancer	1540:1545	arg1	cells					1547:1551	human cervical cancer cells	1525:1551	human cervical cancer cells (Hela)	1525:1558	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	0	17	from	Branches	76:83	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	17	from	Branches	76:83	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	17	from	Branches	76:83	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	17	from	Branches	76:83	arg1	Polysaccharides					55:69	Polysaccharides	55:69	Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	55:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	17	from	Branches	76:83	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	18	theme	Z.	121:122	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	11	19	theme	inhibitors	1790:1799	arg1	polysaccharide					1729:1742	Pe4 polysaccharide	1725:1742	Pe4 polysaccharide from TCBL	1725:1752	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	11	19	theme	inhibitors	1790:1799	arg1	source					1770:1775	a beneficial source	1757:1775	a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity	1757:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	7	20	theme	purified	1219:1226	arg1	polysaccharides					1228:1242	the four purified polysaccharides	1210:1242	the four purified polysaccharides from TCBL	1210:1252	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	0	21	contain	has	213:215	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	21	contain	has	213:215	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	21	contain	has	213:215	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	21	contain	has	213:215	arg2	activities					236:245	various biological activities	217:245	various biological activities	217:245	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	21	contain	has	213:215	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	10	22	theme	Pe4	1598:1600	arg1	polysaccharide					1602:1615	Pe4 polysaccharide	1598:1615	Pe4 polysaccharide	1598:1615	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	4	23	from	TCBL	957:960	arg1	polysaccharides					915:929	The four purified polysaccharides	897:929	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL	897:960	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	2	24	theme	extraction	676:685	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	10	25	theme	inhibitory	1651:1660	arg1	activity					1662:1669	a good α-glucosidase inhibitory activity	1630:1669	a good α-glucosidase inhibitory activity	1630:1669	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	0	26	dep	cuspidata	130:138	arg1	S.					140:141	S.	140:141	S.	140:141	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	27	from	Leaves	89:94	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	27	from	Leaves	89:94	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	27	from	Leaves	89:94	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	27	from	Leaves	89:94	arg1	Polysaccharides					55:69	Polysaccharides	55:69	Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	55:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	27	from	Leaves	89:94	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	28	theme	Polysaccharides	55:69	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	28	theme	Polysaccharides	55:69	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	28	theme	Polysaccharides	55:69	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	28	theme	Polysaccharides	55:69	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	10	29	theme	good	1632:1635	arg1	activity					1662:1669	a good α-glucosidase inhibitory activity	1630:1669	a good α-glucosidase inhibitory activity	1630:1669	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	3	30	theme	optimized	804:812	arg1	conditions					814:823	the optimized conditions	800:823	the optimized conditions	800:823	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	9	31	theme	IC50	1569:1572	arg1	value					1574:1578	its IC50 value	1565:1578	its IC50 value	1565:1578	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	0	32	from	Purification	12:23	arg1	Branches					76:83	Branches	76:83	Branches	76:83	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	32	from	Purification	12:23	arg1	Leaves					89:94	Leaves	89:94	Leaves	89:94	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	2	33	theme	min	698:700	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	33	theme	min	698:700	arg1	ratio					705:709	a ratio	703:709	a ratio of material to liquid of 1:19 (g:mL)	703:746	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	33	theme	min	698:700	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	33	theme	min	698:700	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	0	34	from	Extraction	0:9	arg1	Branches					76:83	Branches	76:83	Branches	76:83	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	34	from	Extraction	0:9	arg1	Leaves					89:94	Leaves	89:94	Leaves	89:94	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	35	theme	activities	333:342	arg1	effect					296:301	the effect	292:301	the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	292:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	0	36	theme	biological	225:234	arg1	activities					236:245	various biological activities	217:245	various biological activities	217:245	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	3	37	theme	polysaccharide	830:843	arg1	yield					845:849	the polysaccharide yield	826:849	the polysaccharide yield from TCBL obtained by EUCE	826:876	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	3	37	theme	polysaccharide	830:843	arg1	%					894:894	4.78% ± 0.18%	882:894	4.78% ± 0.18%	882:894	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	0	38	from	Activities	41:50	arg1	Branches					76:83	Branches	76:83	Branches	76:83	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	38	from	Activities	41:50	arg1	Leaves					89:94	Leaves	89:94	Leaves	89:94	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	39	theme	coupled	545:551	arg1	method					571:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	0	40	theme	Taxus	99:103	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	41	theme	polysaccharides	347:361	arg1	activities					333:342	antidiabetic and antitumor activities	306:342	antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	306:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	8	42	theme	highest	1377:1383	arg1	capacity					1395:1402	the highest antitumor capacity	1373:1402	the highest antitumor capacity	1373:1402	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	1	43	theme	study	270:274	arg1	objective					252:260	The objective	248:260	The objective of this study	248:274	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	8	44	theme	MCF7	1412:1415	arg1	cells					1417:1421	MCF7 cells	1412:1421	MCF7 cells	1412:1421	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	3	45	theme	4.78	882:885	arg1	%					886:886	%	886:886	%	886:886	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	6	46	from	TCBL	1176:1179	arg1	polysaccharides					1155:1169	polysaccharides	1155:1169	polysaccharides from TCBL	1155:1179	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	6	46	from	TCBL	1176:1179	arg1	activity					1120:1127	antidiabetic activity	1107:1127	antidiabetic activity	1107:1127	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	6	46	from	TCBL	1176:1179	arg1	activity					1143:1150	antitumor activity	1133:1150	antitumor activity	1133:1150	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	2	47	dep	g	742:742	arg1	mL					744:745	mL	744:745	g:mL	742:745	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	11	48	theme	type	1804:1807	arg1	diabetes					1812:1819	type II diabetes	1804:1819	type II diabetes	1804:1819	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	1	49	theme	low-temperature	478:492	arg1	enzyme					514:519	a low-temperature and high-efficiency enzyme	476:519	a low-temperature and high-efficiency enzyme	476:519	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	2	50	dep	1:19	736:739	arg1	g					742:742	g	742:742	g:mL	742:745	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	51	theme	mg·mL-1	785:791	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	51	theme	mg·mL-1	785:791	arg1	ratio					705:709	a ratio	703:709	a ratio of material to liquid of 1:19 (g:mL)	703:746	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	51	theme	mg·mL-1	785:791	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	51	theme	mg·mL-1	785:791	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	10	52	theme	Hela	1702:1705	arg1	cells					1707:1711	Hela cells	1702:1711	Hela cells	1702:1711	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	1	53	theme	high-efficiency	498:512	arg1	enzyme					514:519	a low-temperature and high-efficiency enzyme	476:519	a low-temperature and high-efficiency enzyme	476:519	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	6	54	from	activity	1143:1150	arg1	TCBL					1176:1179	TCBL	1176:1179	TCBL	1176:1179	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	7	55	contain	had	1268:1270	arg1	Pe4					1264:1266	purified Pe4	1255:1266	purified Pe4	1255:1266	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	7	55	contain	had	1268:1270	arg2	capacity					1295:1302	the highest inhibitory capacity	1272:1302	the highest inhibitory capacity	1272:1302	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	4	56	dep	polysaccharides	915:929	arg1	Pe4					947:949	Pe4	947:949	Pe4	947:949	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	56	dep	polysaccharides	915:929	arg1	Pe2					937:939	Pe2	937:939	Pe2	937:939	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	0	57	theme	natural	165:171	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	57	theme	natural	165:171	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	57	theme	natural	165:171	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	57	theme	natural	165:171	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	11	58	theme	cervical	1831:1838	arg1	cancer					1840:1845	human cervical cancer	1825:1845	human cervical cancer activity	1825:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	4	59	theme	small	1018:1022	arg1	xylose					1034:1039	xylose	1034:1039	xylose	1034:1039	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	59	theme	small	1018:1022	arg1	amount					1024:1029	a small amount	1016:1029	a small amount of xylose	1016:1039	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	6	60	dep	activity	1120:1127	arg1	The					1103:1105	The	1103:1105	The	1103:1105	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	2	61	theme	Optimal	579:585	arg1	parameters					598:607	Optimal technology parameters	579:607	Optimal technology parameters	579:607	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	61	theme	Optimal	579:585	arg1	follows					628:634	follows	628:634	follows	628:634	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	6	62	theme	antidiabetic	1107:1118	arg1	activity					1120:1127	antidiabetic activity	1107:1127	antidiabetic activity	1107:1127	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	9	63	theme	antitumor	1505:1513	arg1	effect					1515:1520	the highest antitumor effect	1493:1520	the highest antitumor effect	1493:1520	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	7	64	theme	highest	1276:1282	arg1	capacity					1295:1302	the highest inhibitory capacity	1272:1302	the highest inhibitory capacity	1272:1302	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	8	65	contain	had	1369:1371	arg2	capacity					1395:1402	the highest antitumor capacity	1373:1402	the highest antitumor capacity	1373:1402	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	8	65	contain	had	1369:1371	arg1	Pe1					1365:1367	Pe1	1365:1367	Pe1	1365:1367	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	0	66	theme	polysaccharides	193:207	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	66	theme	polysaccharides	193:207	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	66	theme	polysaccharides	193:207	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	66	theme	polysaccharides	193:207	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	67	theme	extraction	440:449	arg1	technology					451:460	the optimum extraction technology	428:460	the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme	428:519	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	0	68	theme	et	118:119	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	11	69	theme	activity	1847:1854	arg1	inhibitors					1790:1799	potential inhibitors	1780:1799	potential inhibitors of type II diabetes and human cervical cancer activity	1780:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	2	70	dep	follows	628:634	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	70	dep	follows	628:634	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	70	dep	follows	628:634	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	70	dep	follows	628:634	arg1	ratio					705:709	a ratio	703:709	a ratio of material to liquid of 1:19 (g:mL)	703:746	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	9	71	theme	cervical	1531:1538	arg1	Hela					1554:1557	Hela	1554:1557	Hela	1554:1557	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	9	71	theme	cervical	1531:1538	arg1	cells					1547:1551	human cervical cancer cells	1525:1551	human cervical cancer cells (Hela)	1525:1558	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	7	72	theme	purified	1255:1262	arg1	Pe4					1264:1266	purified Pe4	1255:1266	purified Pe4	1255:1266	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	2	73	theme	1:19	736:739	arg1	liquid					726:731	liquid	726:731	liquid	726:731	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	0	74	theme	Taxus	124:128	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	1	75	theme	cuspidata	374:382	arg1	branches					384:391	Taxus cuspidata branches	368:391	Taxus cuspidata branches	368:391	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	11	76	theme	potential	1780:1788	arg1	inhibitors					1790:1799	potential inhibitors	1780:1799	potential inhibitors of type II diabetes and human cervical cancer activity	1780:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	3	77	from	TCBL	856:859	arg1	yield					845:849	the polysaccharide yield	826:849	the polysaccharide yield from TCBL obtained by EUCE	826:876	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	3	77	from	TCBL	856:859	arg1	%					894:894	4.78% ± 0.18%	882:894	4.78% ± 0.18%	882:894	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	4	78	theme	xylose	1034:1039	arg1	galactose					996:1004	galactose	996:1004	galactose	996:1004	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	78	theme	xylose	1034:1039	arg1	glucose					1007:1013	glucose	1007:1013	glucose	1007:1013	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	78	theme	xylose	1034:1039	arg1	xylose					1034:1039	xylose	1034:1039	xylose	1034:1039	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	78	theme	xylose	1034:1039	arg1	arabinose					985:993	arabinose	985:993	arabinose	985:993	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	78	theme	xylose	1034:1039	arg1	mannose					1046:1052	mannose	1046:1052	mannose	1046:1052	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	4	78	theme	xylose	1034:1039	arg1	amount					1024:1029	a small amount	1016:1029	a small amount of xylose	1016:1039	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	6	79	theme	polysaccharides	1155:1169	arg1	activity					1120:1127	antidiabetic activity	1107:1127	antidiabetic activity	1107:1127	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	6	79	theme	polysaccharides	1155:1169	arg1	activity					1143:1150	antitumor activity	1133:1150	antitumor activity	1133:1150	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	0	80	dep	S.	140:141	arg1	et					143:144	S. et Z.	140:147	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	80	dep	S.	140:141	arg1	Z.					146:147	Z.	146:147	Z.	146:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	10	81	theme	α-glucosidase	1637:1649	arg1	activity					1662:1669	a good α-glucosidase inhibitory activity	1630:1669	a good α-glucosidase inhibitory activity	1630:1669	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	2	82	theme	material	714:721	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	82	theme	material	714:721	arg1	ratio					705:709	a ratio	703:709	a ratio of material to liquid of 1:19 (g:mL)	703:746	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	82	theme	material	714:721	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	82	theme	material	714:721	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	1	83	from	leaves	397:402	arg1	polysaccharides					347:361	polysaccharides	347:361	polysaccharides from Taxus cuspidata branches and leaves (TCBL)	347:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	83	from	leaves	397:402	arg1	activities					333:342	antidiabetic and antitumor activities	306:342	antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	306:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	84	theme	antidiabetic	306:317	arg1	activities					333:342	antidiabetic and antitumor activities	306:342	antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	306:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	85	from	branches	384:391	arg1	polysaccharides					347:361	polysaccharides	347:361	polysaccharides from Taxus cuspidata branches and leaves (TCBL)	347:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	85	from	branches	384:391	arg1	activities					333:342	antidiabetic and antitumor activities	306:342	antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	306:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	86	theme	antitumor	323:331	arg1	activities					333:342	antidiabetic and antitumor activities	306:342	antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL)	306:409	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	2	87	theme	extraction	640:649	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	1	88	theme	ultrasound-assisted	525:543	arg1	method					571:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	0	89	theme	cuspidata	105:113	arg1	cuspidata					130:138	Taxus cuspidata S. et Z. Taxus cuspidata	99:138	Taxus cuspidata S. et Z. Taxus cuspidata S. et Z.	99:147	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	90	theme	various	217:223	arg1	activities					236:245	various biological activities	217:245	various biological activities	217:245	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	8	91	theme	antitumor	1385:1393	arg1	capacity					1395:1402	the highest antitumor capacity	1373:1402	the highest antitumor capacity	1373:1402	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	1	92	theme	extraction	553:562	arg1	method					571:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	9	93	contain	had	1489:1491	arg2	effect					1515:1520	the highest antitumor effect	1493:1520	the highest antitumor effect	1493:1520	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	9	93	contain	had	1489:1491	arg1	Pe4					1485:1487	Pe4	1485:1487	Pe4	1485:1487	Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL-1.					
31412567	2	94	theme	°C	669:670	arg1	temperature					651:661	an extraction temperature	637:661	an extraction temperature of 51 °C	637:670	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	94	theme	°C	669:670	arg1	ratio					705:709	a ratio	703:709	a ratio of material to liquid of 1:19 (g:mL)	703:746	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	94	theme	°C	669:670	arg1	time					687:690	an extraction time	673:690	an extraction time of 33 min	673:700	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	2	94	theme	°C	669:670	arg1	concentration					763:775	an enzyme concentration	753:775	an enzyme concentration of 0.10 mg·mL-1	753:791	Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL-1.					
31412567	1	95	theme	EUCE	565:568	arg1	method					571:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	11	96	theme	Pe4	1725:1727	arg1	polysaccharide					1729:1742	Pe4 polysaccharide	1725:1742	Pe4 polysaccharide from TCBL	1725:1752	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	11	96	theme	Pe4	1725:1727	arg1	source					1770:1775	a beneficial source	1757:1775	a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity	1757:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	1	97	from	activities	333:342	arg1	branches					384:391	Taxus cuspidata branches	368:391	Taxus cuspidata branches	368:391	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	97	from	activities	333:342	arg1	TCBL					405:408	TCBL	405:408	TCBL	405:408	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	97	from	activities	333:342	arg1	leaves					397:402	leaves	397:402	leaves	397:402	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	11	98	theme	beneficial	1759:1768	arg1	polysaccharide					1729:1742	Pe4 polysaccharide	1725:1742	Pe4 polysaccharide from TCBL	1725:1752	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	11	98	theme	beneficial	1759:1768	arg1	source					1770:1775	a beneficial source	1757:1775	a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity	1757:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	0	99	theme	Biological	30:39	arg1	Purification					12:23	Purification	12:23	Purification	12:23	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	99	theme	Biological	30:39	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	99	theme	Biological	30:39	arg1	Activities					41:50	Biological Activities	30:50	Biological Activities	30:50	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	0	99	theme	Biological	30:39	arg1	source					173:178	an excellent natural source	152:178	an excellent natural source of bioactive polysaccharides	152:207	Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z. Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities.					
31412567	7	100	from	TCBL	1249:1252	arg1	polysaccharides					1228:1242	the four purified polysaccharides	1210:1242	the four purified polysaccharides from TCBL	1210:1252	Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL-1.					
31412567	8	101	theme	HepG2	1427:1431	arg1	cells					1433:1437	HepG2 cells	1427:1437	HepG2 cells	1427:1437	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	10	102	theme	antitumor	1675:1683	arg1	capacity					1685:1692	antitumor capacity	1675:1692	antitumor capacity	1675:1692	Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells.					
31412567	1	103	theme	TCBL	465:468	arg1	method					571:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	ultrasound-assisted coupled extraction (EUCE) method	525:576	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	1	103	theme	TCBL	465:468	arg1	technology					451:460	the optimum extraction technology	428:460	the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme	428:519	The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method.					
31412567	3	104	theme	%	886:886	arg1	yield					845:849	the polysaccharide yield	826:849	the polysaccharide yield from TCBL obtained by EUCE	826:876	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	3	104	theme	%	886:886	arg1	%					894:894	4.78% ± 0.18%	882:894	4.78% ± 0.18%	882:894	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	4	105	theme	purified	906:913	arg1	polysaccharides					915:929	The four purified polysaccharides	897:929	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL	897:960	The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose.					
31412567	3	106	theme	±	888:888	arg1	yield					845:849	the polysaccharide yield	826:849	the polysaccharide yield from TCBL obtained by EUCE	826:876	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	3	106	theme	±	888:888	arg1	%					894:894	4.78% ± 0.18%	882:894	4.78% ± 0.18%	882:894	Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%.					
31412567	8	107	theme	IC50	1445:1448	arg1	values					1450:1455	IC50 values	1445:1455	IC50 values of 169.0 and 132.0 µg·mL-1	1445:1482	Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL-1.					
31412567	11	108	from	TCBL	1749:1752	arg1	polysaccharide					1729:1742	Pe4 polysaccharide	1725:1742	Pe4 polysaccharide from TCBL	1725:1752	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	11	108	from	TCBL	1749:1752	arg1	source					1770:1775	a beneficial source	1757:1775	a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity	1757:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	11	109	theme	diabetes	1812:1819	arg1	inhibitors					1790:1799	potential inhibitors	1780:1799	potential inhibitors of type II diabetes and human cervical cancer activity	1780:1854	Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.					
31412567	6	110	from	activity	1120:1127	arg1	TCBL					1176:1179	TCBL	1176:1179	TCBL	1176:1179	The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro.					
31412567	5	111	theme	HPIC	1088:1091	arg1	analysis					1093:1100	HPIC analysis	1088:1100	HPIC analysis	1088:1100	This composition was assessed by HPIC analysis.					
31887339	0	0	theme	patients	88:95	arg1	cohort					52:57	cohort	52:57	cohort of Chinese colorectal cancer patients	52:95	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	11	1	theme	first	1403:1407	arg1	This					1391:1394	This	1391:1394	This	1391:1394	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	11	1	theme	first	1403:1407	arg1	comprehensively					1409:1423	the first comprehensively	1399:1423	the first comprehensively	1399:1423	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	1	2	theme	cancer	167:172	arg1	mortality					143:151	mortality	143:151	mortality	143:151	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	1	2	theme	cancer	167:172	arg1	morbidity					129:137	morbidity	129:137	morbidity	129:137	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	0	3	theme	cancer	81:86	arg1	patients					88:95	Chinese colorectal cancer patients	62:95	Chinese colorectal cancer patients	62:95	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	8	4	theme	glycans	1044:1050	arg1	fucosylation					1017:1028	fucosylation	1017:1028	fucosylation	1017:1028	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	8	4	theme	glycans	1044:1050	arg1	sialylation					1001:1011	IgG sialylation	997:1011	IgG sialylation	997:1011	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	1	5	dep	BACKGROUD	98:106	arg1	mortality					143:151	mortality	143:151	mortality	143:151	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	1	5	dep	BACKGROUD	98:106	arg1	morbidity					129:137	morbidity	129:137	morbidity	129:137	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	1	6	theme	early	242:246	arg1	diagnosis					248:256	early diagnosis	242:256	early diagnosis of CRC	242:263	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	0	7	from	Profiling	0:8	arg1	cohort					52:57	cohort	52:57	cohort of Chinese colorectal cancer patients	52:95	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	5	8	theme	K-S	633:635	arg1	test					637:640	K-S test	633:640	K-S test	633:640	Statistical analysis including K-S test, t-test, ROC curve and OPLS-DA were successively performed.					
31887339	2	9	theme	IgG	274:276	arg1	glycosylation					278:290	Altered IgG glycosylation	266:290	Altered IgG glycosylation	266:290	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	13	10	theme	cancer	1685:1690	arg1	progression					1692:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	12	11	theme	early	1629:1633	arg1	diagnosis					1635:1643	early diagnosis	1629:1643	early diagnosis of CRC	1629:1650	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
31887339	13	12	theme	GENERAL	1653:1659	arg1	progression					1692:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	11	13	theme	disease	1533:1539	arg1	patients					1541:1548	colorectal disease patients	1522:1548	colorectal disease patients	1522:1548	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	2	14	theme	Altered	266:272	arg1	glycosylation					278:290	Altered IgG glycosylation	266:290	Altered IgG glycosylation	266:290	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	3	15	theme	PGC-based	478:486	arg1	analysis					505:512	PGC-based nanoLC-ESI-MS/MS analysis	478:512	PGC-based nanoLC-ESI-MS/MS analysis	478:512	METHODS Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis.					
31887339	11	16	theme	isomer-specific	1438:1452	arg1	N-glycosylation					1458:1472	isomer-specific IgG N-glycosylation	1438:1472	isomer-specific IgG N-glycosylation	1438:1472	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	3	17	theme	nanoLC-ESI-MS/MS	488:503	arg1	analysis					505:512	PGC-based nanoLC-ESI-MS/MS analysis	478:512	PGC-based nanoLC-ESI-MS/MS analysis	478:512	METHODS Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis.					
31887339	11	18	dep	CONCLUSIONS	1379:1389	arg1	This					1391:1394	This	1391:1394	This	1391:1394	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	11	18	dep	CONCLUSIONS	1379:1389	arg1	comprehensively					1409:1423	the first comprehensively	1399:1423	the first comprehensively	1399:1423	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	8	19	theme	IgG	997:999	arg1	sialylation					1001:1011	IgG sialylation	997:1011	IgG sialylation	997:1011	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	3	20	theme	METHODS	414:420	arg1	Methylamidation					422:436	METHODS Methylamidation	414:436	METHODS Methylamidation of IgG N-glycans	414:453	METHODS Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis.					
31887339	8	21	from	increase	951:958	arg1	agalactosylation					967:982	IgG agalactosylation	963:982	IgG agalactosylation	963:982	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	11	22	theme	IgG	1454:1456	arg1	N-glycosylation					1458:1472	isomer-specific IgG N-glycosylation	1438:1472	isomer-specific IgG N-glycosylation	1438:1472	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	9	23	theme	accurate	1210:1217	arg1	area					1219:1222	an accurate area	1207:1222	an accurate area under the receiver operating characteristic curve	1207:1272	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	6	24	theme	independent	719:729	arg1	set					731:733	an independent set	716:733	an independent set	716:733	Additionally, an independent set was utilized to validate the results.					
31887339	3	25	theme	IgG	441:443	arg1	N-glycans					445:453	IgG N-glycans	441:453	IgG N-glycans	441:453	METHODS Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis.					
31887339	6	26	used	utilized	739:746	arg2	set					731:733	an independent set	716:733	an independent set	716:733	Additionally, an independent set was utilized to validate the results.					
31887339	5	27	theme	ROC	651:653	arg1	curve					655:659	ROC curve	651:659	ROC curve	651:659	Statistical analysis including K-S test, t-test, ROC curve and OPLS-DA were successively performed.					
31887339	11	28	from	patients	1541:1548	arg1	controls					1508:1515	normal controls	1501:1515	normal controls from colorectal disease patients	1501:1548	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	2	29	theme	glycosylation	368:380	arg1	association					340:350	the association	336:350	the association of IgG isomeric glycosylation with CRC	336:389	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	1	30	theme	CRC	261:263	arg1	diagnosis					248:256	early diagnosis	242:256	early diagnosis of CRC	242:263	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	5	31	theme	Statistical	602:612	arg1	analysis					614:621	Statistical analysis	602:621	Statistical analysis including K-S test, t-test, ROC curve and OPLS-DA	602:671	Statistical analysis including K-S test, t-test, ROC curve and OPLS-DA were successively performed.					
31887339	13	32	theme	SIGNIFICANCE	1661:1672	arg1	progression					1692:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	11	33	theme	colorectal	1522:1531	arg1	patients					1541:1548	colorectal disease patients	1522:1548	colorectal disease patients	1522:1548	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	2	34	theme	isomeric	359:366	arg1	glycosylation					368:380	IgG isomeric glycosylation	355:380	IgG isomeric glycosylation	355:380	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	0	35	theme	IgG	29:31	arg1	N-glycosylation					33:47	isomer-specific IgG N-glycosylation	13:47	isomer-specific IgG N-glycosylation	13:47	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	8	36	theme	sialylated	1033:1042	arg1	glycans					1044:1050	sialylated glycans	1033:1050	sialylated glycans	1033:1050	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	13	37	theme	Colorectal	1674:1683	arg1	progression					1692:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression	1653:1702	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	8	38	theme	Statistical	898:908	arg1	analysis					910:917	Statistical analysis	898:917	Statistical analysis	898:917	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	0	39	theme	isomer-specific	13:27	arg1	N-glycosylation					33:47	isomer-specific IgG N-glycosylation	13:47	isomer-specific IgG N-glycosylation	13:47	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	4	40	theme	MATLAB	585:590	arg1	solution					592:599	MATLAB solution	585:599	MATLAB solution	585:599	Data processing was operated by a self-developed application based on MATLAB solution.					
31887339	12	41	theme	screening	1604:1612	arg1	indicators					1614:1623	important screening indicators	1594:1623	important screening indicators for early diagnosis of CRC	1594:1650	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
31887339	10	42	theme	independent	1325:1335	arg1	set					1337:1339	an independent set	1322:1339	an independent set	1322:1339	Significantly, these results were validated in an independent set by multivariate statistical analysis.					
31887339	7	43	theme	compositional	809:821	arg1	isomers					823:829	79 compositional isomers	806:829	79 compositional isomers	806:829	RESULTS Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far.					
31887339	9	44	theme	operating	1243:1251	arg1	curve					1268:1272	the receiver operating characteristic curve	1230:1272	the receiver operating characteristic curve	1230:1272	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	3	45	theme	N-glycans	445:453	arg1	Methylamidation					422:436	METHODS Methylamidation	414:436	METHODS Methylamidation of IgG N-glycans	414:453	METHODS Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis.					
31887339	0	46	theme	N-glycosylation	33:47	arg1	Profiling					0:8	Profiling	0:8	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.	0:96	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	8	47	gly	fucosylation	1017:1028	arg1	glycans					1044:1050	sialylated glycans	1033:1050	sialylated glycans	1033:1050	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	7	48	dep	RESULTS	773:779	arg1	isomers					823:829	79 compositional isomers	806:829	79 compositional isomers	806:829	RESULTS Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far.					
31887339	7	48	dep	RESULTS	773:779	arg1	Total					781:785	Total 28 IgG glycans and 79 compositional isomers	781:829	Total	781:785	RESULTS Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far.					
31887339	9	49	dep	isomers	1105:1111	arg1	isomers					1105:1111	three compositional isomers	1085:1111	three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e)	1085:1147	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	49	dep	isomers	1105:1111	arg1	H4N3S1F1-e					1137:1146	H4N3S1F1-e	1137:1146	H4N3S1F1-e	1137:1146	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	49	dep	isomers	1105:1111	arg1	H3N4F1-b					1124:1131	H3N4F1-b	1124:1131	H3N4F1-b	1124:1131	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	49	dep	isomers	1105:1111	arg1	H3N4F1-a					1114:1121	H3N4F1-a	1114:1121	H3N4F1-a	1114:1121	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	50	with	controls	1193:1200	arg1	area					1219:1222	an accurate area	1207:1222	an accurate area under the receiver operating characteristic curve	1207:1272	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	12	51	theme	important	1594:1602	arg1	indicators					1614:1623	important screening indicators	1594:1623	important screening indicators for early diagnosis of CRC	1594:1650	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
31887339	7	52	dep	Total	781:785	arg1	glycans					794:800	28 IgG glycans	787:800	28 IgG glycans	787:800	RESULTS Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far.					
31887339	9	53	theme	characteristic	1253:1266	arg1	curve					1268:1272	the receiver operating characteristic curve	1230:1272	the receiver operating characteristic curve	1230:1272	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	1	54	theme	increasing	118:127	arg1	morbidity					129:137	morbidity	129:137	morbidity	129:137	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	8	55	gly	sialylated	1033:1042	arg1	glycans					1044:1050	sialylated glycans	1033:1050	sialylated glycans	1033:1050	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	4	56	theme	self-developed	549:562	arg1	application					564:574	a self-developed application	547:574	a self-developed application based on MATLAB solution	547:599	Data processing was operated by a self-developed application based on MATLAB solution.					
31887339	13	57	theme	isomer-specific	1732:1746	arg1	N-glycosylation					1752:1766	isomer-specific IgG N-glycosylation	1732:1766	isomer-specific IgG N-glycosylation	1732:1766	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	2	58	with	association	340:350	arg1	CRC					387:389	CRC	387:389	CRC	387:389	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	10	59	theme	statistical	1357:1367	arg1	analysis					1369:1376	multivariate statistical analysis	1344:1376	multivariate statistical analysis	1344:1376	Significantly, these results were validated in an independent set by multivariate statistical analysis.					
31887339	9	60	theme	compositional	1091:1103	arg1	isomers					1105:1111	three compositional isomers	1085:1111	three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e)	1085:1147	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	60	theme	compositional	1091:1103	arg1	H4N3S1F1-e					1137:1146	H4N3S1F1-e	1137:1146	H4N3S1F1-e	1137:1146	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	60	theme	compositional	1091:1103	arg1	H3N4F1-b					1124:1131	H3N4F1-b	1124:1131	H3N4F1-b	1124:1131	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	9	60	theme	compositional	1091:1103	arg1	H3N4F1-a					1114:1121	H3N4F1-a	1114:1121	H3N4F1-a	1114:1121	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	12	61	theme	IgG	1565:1567	arg1	glyco-biomarkers					1569:1584	The candidate IgG glyco-biomarkers	1551:1584	The candidate IgG glyco-biomarkers	1551:1584	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
31887339	7	62	theme	IgG	790:792	arg1	glycans					794:800	28 IgG glycans	787:800	28 IgG glycans	787:800	RESULTS Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far.					
31887339	13	63	theme	IgG	1748:1750	arg1	N-glycosylation					1752:1766	isomer-specific IgG N-glycosylation	1732:1766	isomer-specific IgG N-glycosylation	1732:1766	GENERAL SIGNIFICANCE Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.					
31887339	10	64	theme	multivariate	1344:1355	arg1	analysis					1369:1376	multivariate statistical analysis	1344:1376	multivariate statistical analysis	1344:1376	Significantly, these results were validated in an independent set by multivariate statistical analysis.					
31887339	11	65	theme	normal	1501:1506	arg1	controls					1508:1515	normal controls	1501:1515	normal controls from colorectal disease patients	1501:1548	CONCLUSIONS This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients.					
31887339	12	66	theme	candidate	1555:1563	arg1	glyco-biomarkers					1569:1584	The candidate IgG glyco-biomarkers	1551:1584	The candidate IgG glyco-biomarkers	1551:1584	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
31887339	8	67	theme	IgG	963:965	arg1	agalactosylation					967:982	IgG agalactosylation	963:982	IgG agalactosylation	963:982	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	2	68	theme	CRC	311:313	arg1	progression					315:325	CRC progression	311:325	CRC progression	311:325	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	1	69	theme	noninvasive	207:217	arg1	strategy					229:236	a noninvasive screening strategy	205:236	a noninvasive screening strategy for early diagnosis of CRC	205:263	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	0	70	theme	colorectal	70:79	arg1	patients					88:95	Chinese colorectal cancer patients	62:95	Chinese colorectal cancer patients	62:95	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	2	71	theme	IgG	355:357	arg1	glycosylation					368:380	IgG isomeric glycosylation	355:380	IgG isomeric glycosylation	355:380	Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated.					
31887339	1	72	theme	screening	219:227	arg1	strategy					229:236	a noninvasive screening strategy	205:236	a noninvasive screening strategy for early diagnosis of CRC	205:263	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	0	73	theme	Chinese	62:68	arg1	patients					88:95	Chinese colorectal cancer patients	62:95	Chinese colorectal cancer patients	62:95	Profiling of isomer-specific IgG N-glycosylation in cohort of Chinese colorectal cancer patients.					
31887339	4	74	theme	Data	515:518	arg1	processing					520:529	Data processing	515:529	Data processing	515:529	Data processing was operated by a self-developed application based on MATLAB solution.					
31887339	8	75	gly	sialylation	1001:1011	arg1	glycans					1044:1050	sialylated glycans	1033:1050	sialylated glycans	1033:1050	Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans.					
31887339	9	76	theme	early	1175:1179	arg1	stages					1181:1186	early stages	1175:1186	early stages	1175:1186	Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve.					
31887339	1	77	theme	colorectal	156:165	arg1	CRC					175:177	CRC	175:177	CRC	175:177	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	1	77	theme	colorectal	156:165	arg1	cancer					167:172	colorectal cancer	156:172	colorectal cancer (CRC)	156:178	BACKGROUD Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC.					
31887339	12	78	theme	CRC	1648:1650	arg1	diagnosis					1635:1643	early diagnosis	1629:1643	early diagnosis of CRC	1629:1650	The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC.					
29898109	0	0	theme	different	88:96	arg1	abilities					116:124	different biofilm formation abilities	88:124	different biofilm formation abilities	88:124	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	11	1	theme	Amphora	1280:1286	arg1	sp					1288:1289	Amphora sp	1280:1289	Amphora sp	1280:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	7	2	dep	less	848:851	arg1	<0.4-fold					854:862	<0.4-fold	854:862	<0.4-fold	854:862	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	1	3	theme	benthic	158:164	arg1	diatoms					166:172	two benthic diatoms	154:172	two benthic diatoms	154:172	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	11	4	from	monomer	1259:1265	arg1	SL-EPS					1270:1275	SL-EPS	1270:1275	SL-EPS of Amphora sp	1270:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	3	5	theme	diatom	342:347	arg1	abilities					367:375	the diatom biofilm formation abilities	338:375	the diatom biofilm formation abilities	338:375	The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities.					
29898109	4	6	from	cells	515:519	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	6	from	cells	515:519	arg1	EPS					498:500	loosely and tightly bound EPS	472:500	loosely and tightly bound EPS from biofilm cells	472:519	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	7	theme	whole	418:422	arg1	culture					424:430	whole culture	418:430	whole culture	418:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	1	8	theme	diatoms	166:172	arg1	EPS					146:148	EPS	146:148	EPS	146:148	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	1	8	theme	diatoms	166:172	arg1	sp					183:184	Amphora sp	175:184	Amphora sp	175:184	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	1	8	theme	diatoms	166:172	arg1	Exopolysaccharide					127:143	Exopolysaccharide	127:143	Exopolysaccharide (EPS) of two benthic diatoms	127:172	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	10	9	theme	Amphora	1133:1139	arg1	sp					1141:1142	Amphora sp	1133:1142	Amphora sp	1133:1142	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	0	10	theme	formation	106:114	arg1	abilities					116:124	different biofilm formation abilities	88:124	different biofilm formation abilities	88:124	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	11	11	located	present	1177:1183	arg2	hexosamine					1161:1170	hexosamine	1161:1170	hexosamine	1161:1170	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	11	11	located	present	1177:1183	arg1	fractions					1192:1200	all fractions	1188:1200	all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp	1188:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	11	11	located	present	1177:1183	arg2	acid					1152:1155	Uronic acid	1145:1155	Uronic acid	1145:1155	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	7	12	dep	more	802:805	arg1	1.5-fold					808:815	1.5-fold	808:815	1.5-fold	808:815	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	7	13	theme	Stauroneis	880:889	arg1	sp					891:892	Stauroneis sp	880:892	Stauroneis sp	880:892	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	5	14	from	terms	714:718	arg1	different					701:709	different	701:709	different	701:709	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	0	15	theme	biofilm	98:104	arg1	abilities					116:124	different biofilm formation abilities	88:124	different biofilm formation abilities	88:124	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	12	16	theme	strong	1389:1394	arg1	abilities					1414:1422	the strong biofilm formation abilities	1385:1422	the strong biofilm formation abilities of Amphora sp	1385:1436	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	10	17	from	Stauroneis	1081:1090	arg1	abundant					1069:1076	abundant	1069:1076	abundant	1069:1076	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	0	18	with	diatoms	75:81	arg1	abilities					116:124	different biofilm formation abilities	88:124	different biofilm formation abilities	88:124	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	11	19	from	present	1177:1183	arg1	fractions					1192:1200	all fractions	1188:1200	all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp	1188:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	12	20	theme	high	1317:1320	arg1	crucial					1373:1379	crucial	1373:1379	crucial	1373:1379	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	12	20	theme	high	1317:1320	arg1	content					1322:1328	the high content	1313:1328	the high content of uronic acid (especially Glc-A)	1313:1362	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	4	21	theme	culture	424:430	arg1	supernatant					403:413	the supernatant	399:413	the supernatant of whole culture	399:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	8	22	from	sp	939:940	arg1	monosaccharides					899:913	The monosaccharides	895:913	The monosaccharides of the EPS from Amphora sp	895:940	The monosaccharides of the EPS from Amphora sp.					
29898109	12	23	theme	uronic	1333:1338	arg1	acid					1340:1343	uronic acid	1333:1343	uronic acid (especially Glc-A)	1333:1362	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	5	24	theme	monosaccharide	632:645	arg1	compositions					647:658	monosaccharide compositions	632:658	monosaccharide compositions	632:658	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	11	25	theme	abundant	1250:1257	arg1	monomer					1259:1265	the most abundant monomer	1241:1265	the most abundant monomer in SL-EPS of Amphora sp	1241:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	1	26	theme	Amphora	175:181	arg1	sp					183:184	Amphora sp	175:184	Amphora sp	175:184	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	1	26	theme	Amphora	175:181	arg1	Exopolysaccharide					127:143	Exopolysaccharide	127:143	Exopolysaccharide (EPS) of two benthic diatoms	127:172	Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp.					
29898109	9	27	with	diverse	953:959	arg1	monomers					1006:1013	13 and 10 monomers	996:1013	13 and 10 monomers	996:1013	were more diverse than those of Stauroneis sp., with 13 and 10 monomers, respectively.					
29898109	4	28	theme	bound	492:496	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	28	theme	bound	492:496	arg1	EPS					498:500	loosely and tightly bound EPS	472:500	loosely and tightly bound EPS from biofilm cells	472:519	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	11	29	theme	diatoms	1209:1215	arg1	fractions					1192:1200	all fractions	1188:1200	all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp	1188:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	5	30	theme	monomer	759:765	arg1	compositions					767:778	monomer compositions	759:778	monomer compositions	759:778	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	4	31	from	supernatant	403:413	arg1	F-TB-EPS					550:557	F-TB-EPS	550:557	F-TB-EPS	550:557	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	EPS					447:449	tightly bound EPS	433:449	tightly bound EPS from floating cells	433:469	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	BF-LB-EPS					560:568	BF-LB-EPS	560:568	BF-LB-EPS	560:568	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	EPS					498:500	loosely and tightly bound EPS	472:500	loosely and tightly bound EPS from biofilm cells	472:519	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	SL-EPS					542:547	SL-EPS	542:547	SL-EPS	542:547	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	31	from	supernatant	403:413	arg1	BF-TB-EPS					574:582	BF-TB-EPS	574:582	BF-TB-EPS	574:582	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	10	32	dep	Stauroneis	1081:1090	arg1	sp.					1092:1094	Stauroneis sp.	1081:1094	Stauroneis sp.	1081:1094	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	12	33	theme	acid	1340:1343	arg1	crucial					1373:1379	crucial	1373:1379	crucial	1373:1379	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	12	33	theme	acid	1340:1343	arg1	content					1322:1328	the high content	1313:1328	the high content of uronic acid (especially Glc-A)	1313:1362	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	10	34	from	abundant	1069:1076	arg1	Stauroneis					1081:1090	Stauroneis	1081:1090	Stauroneis	1081:1090	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	7	35	theme	total	818:822	arg1	exopolysaccharides					824:841	more (1.5-fold) total exopolysaccharides	802:841	more (1.5-fold) total exopolysaccharides	802:841	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	7	36	theme	less	848:851	arg1	BF-TB-EPS					865:873	less (<0.4-fold) BF-TB-EPS	848:873	less (<0.4-fold) BF-TB-EPS	848:873	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	12	37	theme	formation	1404:1412	arg1	abilities					1414:1422	the strong biofilm formation abilities	1385:1422	the strong biofilm formation abilities of Amphora sp	1385:1436	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	3	38	theme	suspension-cells/biofilm-cells	282:311	arg1	ratio					273:277	The ratio	269:277	The ratio of suspension-cells/biofilm-cells	269:311	The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities.					
29898109	0	39	theme	exopolysaccharides	39:56	arg1	comparison					25:34	comparison	25:34	comparison	25:34	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	0	39	theme	exopolysaccharides	39:56	arg1	characterization					4:19	characterization	4:19	characterization	4:19	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	4	40	from	cells	465:469	arg1	EPS					447:449	tightly bound EPS	433:449	tightly bound EPS from floating cells	433:469	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	40	from	cells	465:469	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	41	theme	floating	456:463	arg1	cells					465:469	floating cells	456:469	floating cells	456:469	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	2	42	theme	formation	230:238	arg1	abilities					240:248	different biofilm formation abilities	212:248	different biofilm formation abilities	212:248	and Stauroneis sp., with different biofilm formation abilities were investigated.					
29898109	5	43	theme	productions	727:737	arg1	terms					714:718	terms	714:718	terms of the productions, distributions, and monomer compositions	714:778	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	0	44	from	diatoms	75:81	arg1	comparison					25:34	comparison	25:34	comparison	25:34	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	0	44	from	diatoms	75:81	arg1	characterization					4:19	characterization	4:19	characterization	4:19	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	2	45	theme	biofilm	222:228	arg1	abilities					240:248	different biofilm formation abilities	212:248	different biofilm formation abilities	212:248	and Stauroneis sp., with different biofilm formation abilities were investigated.					
29898109	6	46	theme	Amphora	781:787	arg1	sp					789:790	Amphora sp	781:790	Amphora sp.	781:791	Amphora sp.					
29898109	7	47	theme	more	802:805	arg1	exopolysaccharides					824:841	more (1.5-fold) total exopolysaccharides	802:841	more (1.5-fold) total exopolysaccharides	802:841	produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp.					
29898109	0	48	theme	benthic	67:73	arg1	diatoms					75:81	two benthic diatoms	63:81	two benthic diatoms with different biofilm formation abilities	63:124	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	2	49	theme	different	212:220	arg1	abilities					240:248	different biofilm formation abilities	212:248	different biofilm formation abilities	212:248	and Stauroneis sp., with different biofilm formation abilities were investigated.					
29898109	8	50	theme	EPS	922:924	arg1	monosaccharides					899:913	The monosaccharides	895:913	The monosaccharides of the EPS from Amphora sp	895:940	The monosaccharides of the EPS from Amphora sp.					
29898109	11	51	from	fractions	1192:1200	arg1	present					1177:1183	present	1177:1183	present	1177:1183	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	11	52	theme	Uronic	1145:1150	arg1	acid					1152:1155	Uronic acid	1145:1155	Uronic acid	1145:1155	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	10	53	from	rich	1125:1128	arg1	sp					1141:1142	Amphora sp	1133:1142	Amphora sp	1133:1142	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	4	54	theme	bound	441:445	arg1	EPS					447:449	tightly bound EPS	433:449	tightly bound EPS from floating cells	433:469	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	54	theme	bound	441:445	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	F-TB-EPS					550:557	F-TB-EPS	550:557	F-TB-EPS	550:557	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	EPS					447:449	tightly bound EPS	433:449	tightly bound EPS from floating cells	433:469	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	BF-LB-EPS					560:568	BF-LB-EPS	560:568	BF-LB-EPS	560:568	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	EPS					498:500	loosely and tightly bound EPS	472:500	loosely and tightly bound EPS from biofilm cells	472:519	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	SL-EPS					542:547	SL-EPS	542:547	SL-EPS	542:547	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	EPS					390:392	The soluble EPS	378:392	The soluble EPS from the supernatant of whole culture	378:430	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	4	55	theme	soluble	382:388	arg1	BF-TB-EPS					574:582	BF-TB-EPS	574:582	BF-TB-EPS	574:582	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	12	56	theme	biofilm	1396:1402	arg1	abilities					1414:1422	the strong biofilm formation abilities	1385:1422	the strong biofilm formation abilities of Amphora sp	1385:1436	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	12	57	dep	acid	1340:1343	arg1	Glc-A					1357:1361	Glc-A	1357:1361	especially Glc-A	1346:1361	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	10	58	from	sp	1141:1142	arg1	rich					1125:1128	rich	1125:1128	rich	1125:1128	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	10	59	theme	Neutral	1030:1036	arg1	Man					1059:1061	Man	1059:1061	Man	1059:1061	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	10	59	theme	Neutral	1030:1036	arg1	Xyl					1051:1053	Xyl	1051:1053	Xyl	1051:1053	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	10	59	theme	Neutral	1030:1036	arg1	Glc					1046:1048	Glc	1046:1048	Glc	1046:1048	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	10	59	theme	Neutral	1030:1036	arg1	sugars					1038:1043	Neutral sugars	1030:1043	Neutral sugars	1030:1043	Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp.					
29898109	12	60	theme	Amphora	1427:1433	arg1	sp					1435:1436	Amphora sp	1427:1436	Amphora sp	1427:1436	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	5	61	theme	compositions	767:778	arg1	terms					714:718	terms	714:718	terms of the productions, distributions, and monomer compositions	714:778	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	11	62	theme	sp	1288:1289	arg1	SL-EPS					1270:1275	SL-EPS	1270:1275	SL-EPS of Amphora sp	1270:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	2	63	dep	Stauroneis	191:200	arg1	sp.					202:204	and Stauroneis sp., with different biofilm formation abilities	187:248	sp.	202:204	and Stauroneis sp., with different biofilm formation abilities were investigated.					
29898109	8	64	theme	Amphora	931:937	arg1	sp					939:940	Amphora sp	931:940	Amphora sp	931:940	The monosaccharides of the EPS from Amphora sp.					
29898109	12	65	theme	sp	1435:1436	arg1	abilities					1414:1422	the strong biofilm formation abilities	1385:1422	the strong biofilm formation abilities of Amphora sp	1385:1436	It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.					
29898109	3	66	theme	biofilm	349:355	arg1	abilities					367:375	the diatom biofilm formation abilities	338:375	the diatom biofilm formation abilities	338:375	The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities.					
29898109	0	67	dep	characterization	4:19	arg1	The					0:2	The	0:2	The	0:2	The characterization and comparison of exopolysaccharides from two benthic diatoms with different biofilm formation abilities.					
29898109	11	68	attach	present	1177:1183	arg2	hexosamine					1161:1170	hexosamine	1161:1170	hexosamine	1161:1170	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	11	68	attach	present	1177:1183	arg1	fractions					1192:1200	all fractions	1188:1200	all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp	1188:1289	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	11	68	attach	present	1177:1183	arg2	acid					1152:1155	Uronic acid	1145:1155	Uronic acid	1145:1155	Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp.					
29898109	5	69	from	diatoms	688:694	arg1	EPS					675:677	EPS	675:677	EPS from two diatoms	675:694	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	3	70	theme	formation	357:365	arg1	abilities					367:375	the diatom biofilm formation abilities	338:375	the diatom biofilm formation abilities	338:375	The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities.					
29898109	4	71	theme	biofilm	507:513	arg1	cells					515:519	biofilm cells	507:519	biofilm cells	507:519	The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively.					
29898109	5	72	from	different	701:709	arg1	terms					714:718	terms	714:718	terms of the productions, distributions, and monomer compositions	714:778	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29898109	5	73	theme	distributions	740:752	arg1	terms					714:718	terms	714:718	terms of the productions, distributions, and monomer compositions	714:778	The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions.					
29727645	5	0	from	10:8.4:7.5	852:861	arg1	SP					866:867	SP	866:867	SP	866:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	1	1	theme	sea	157:159	arg1	algae					161:165	sea algae	157:165	sea algae	157:165	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	8	2	theme	oxygen	1095:1100	arg1	consumption					1102:1112	oxygen consumption	1095:1112	oxygen consumption	1095:1112	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	5	3	theme	Gas	712:714	arg1	GC					731:732	GC	731:732	GC	731:732	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	3	theme	Gas	712:714	arg1	chromatograph					716:728	Gas chromatograph	712:728	Gas chromatograph (GC)	712:733	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	6	4	theme	SPIC	883:886	arg1	MW					870:871	MW	870:871	MW of SP and SPIC	870:886	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	4	5	theme	polysaccharide	690:703	arg1	chain					705:709	polysaccharide chain	690:709	polysaccharide chain	690:709	Fourier-transform infrared (FTIR) spectra indicated further that iron was chelated in polysaccharide chain.					
29727645	3	6	from	difference	488:497	arg1	SPIC					463:466	SPIC	463:466	SPIC	463:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	6	from	difference	488:497	arg1	content					523:529	total polysaccharide content	502:529	total polysaccharide content	502:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	5	7	with	mucose	786:791	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	3	8	theme	protein	549:555	arg1	contents					557:564	protein contents	549:564	protein contents	549:564	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	0	9	theme	iron	99:102	arg1	complex					104:110	its iron complex	95:110	its iron complex	95:110	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	6	10	theme	SP	876:877	arg1	MW					870:871	MW	870:871	MW of SP and SPIC	870:886	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	2	11	theme	active	258:263	arg1	components					265:274	components	265:274	components	265:274	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	3	12	contain	have	416:419	arg2	difference					488:497	no significant difference	473:497	no significant difference in total polysaccharide content	473:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	12	contain	have	416:419	arg2	ions					455:458	no free ferric ions	440:458	no free ferric ions in SPIC	440:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	12	contain	have	416:419	arg1	both					411:414	both	411:414	both	411:414	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	12	contain	have	416:419	arg2	stability					426:434	good stability	421:434	good stability	421:434	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	2	13	dep	components	265:274	arg1	The					249:251	The	249:251	The	249:251	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	6	14	theme	liquid	964:969	arg1	HPLC					987:990	HPLC	987:990	HPLC	987:990	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	6	14	theme	liquid	964:969	arg1	chromatography					971:984	high-performance liquid chromatography	947:984	high-performance liquid chromatography (HPLC)	947:991	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	7	15	contain	had	999:1001	arg2	effects					1032:1038	hydroxyl radicals scavenging effects	1003:1038	hydroxyl radicals scavenging effects	1003:1038	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	7	15	contain	had	999:1001	arg1	Both					994:997	Both	994:997	Both	994:997	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	5	16	from	ratio	820:824	arg1	SPIC					843:846	SPIC	843:846	SPIC	843:846	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	16	from	ratio	820:824	arg1	SP					866:867	SP	866:867	SP	866:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	17	theme	10:3.8:3.5	829:838	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	2	18	theme	main	253:256	arg1	components					265:274	components	265:274	components	265:274	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	8	19	theme	longer	1163:1168	arg1	time					1170:1173	longer time	1163:1173	longer time	1163:1173	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	5	20	theme	10:8.4:7.5	852:861	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	9	21	theme	ROS	1320:1322	arg1	generation					1324:1333	the ROS generation	1316:1333	the ROS generation	1316:1333	Fluorescence extensity of intracellular ROS showed that SP could reduce the ROS generation while SPIC enhances the effect.					
29727645	10	22	theme	SP	1475:1476	arg1	chelating					1478:1486	SP chelating	1475:1486	SP chelating with ferric	1475:1498	The results revealed that the main active components and antioxidant activity exhibit differentiation after SP chelating with ferric.					
29727645	10	23	theme	antioxidant	1424:1434	arg1	activity					1436:1443	antioxidant activity	1424:1443	antioxidant activity	1424:1443	The results revealed that the main active components and antioxidant activity exhibit differentiation after SP chelating with ferric.					
29727645	2	24	theme	monosaccharide	288:301	arg1	composition					303:313	monosaccharide composition	288:313	monosaccharide composition	288:313	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	3	25	theme	good	421:424	arg1	stability					426:434	good stability	421:434	good stability	421:434	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	0	26	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex	0:110	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	6	27	theme	high-performance	947:962	arg1	HPLC					987:990	HPLC	987:990	HPLC	987:990	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	6	27	theme	high-performance	947:962	arg1	chromatography					971:984	high-performance liquid chromatography	947:984	high-performance liquid chromatography (HPLC)	947:991	MW of SP and SPIC varied no more at 231,3 kD and 237.2 kD respectively using high-performance liquid chromatography (HPLC).					
29727645	7	28	theme	stronger	1067:1074	arg1	effects					1076:1082	stronger effects	1067:1082	stronger effects	1067:1082	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	10	29	theme	active	1402:1407	arg1	components					1409:1418	the main active components	1393:1418	the main active components	1393:1418	The results revealed that the main active components and antioxidant activity exhibit differentiation after SP chelating with ferric.					
29727645	5	30	with	fucose	778:783	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	0	31	theme	components	21:30	arg1	study					12:16	Comparative study	0:16	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex	0:110	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	10	32	theme	main	1397:1400	arg1	components					1409:1418	the main active components	1393:1418	the main active components	1393:1418	The results revealed that the main active components and antioxidant activity exhibit differentiation after SP chelating with ferric.					
29727645	5	33	with	glucose	797:803	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	34	from	SP	866:867	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	3	35	theme	polysaccharide	508:521	arg1	content					523:529	total polysaccharide content	502:529	total polysaccharide content	502:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	1	36	theme	Sulfated	113:120	arg1	SP					138:139	SP	138:139	SP	138:139	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	1	36	theme	Sulfated	113:120	arg1	polysaccharide					122:135	Sulfated polysaccharide	113:135	Sulfated polysaccharide (SP) extracted from sea algae	113:165	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	4	37	theme	FTIR	632:635	arg1	spectra					638:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra indicated further that iron was chelated in polysaccharide chain.					
29727645	0	38	theme	anti-oxidative	36:49	arg1	effects					51:57	anti-oxidative effects	36:57	anti-oxidative effects	36:57	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	10	39	with	chelating	1478:1486	arg1	ferric					1493:1498	ferric	1493:1498	ferric	1493:1498	The results revealed that the main active components and antioxidant activity exhibit differentiation after SP chelating with ferric.					
29727645	3	40	theme	free	443:446	arg1	ions					455:458	no free ferric ions	440:458	no free ferric ions in SPIC	440:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	41	theme	increasing	592:601	arg1	content					584:590	and iron content increasing	575:601	content	584:590	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	42	theme	ferric	448:453	arg1	ions					455:458	no free ferric ions	440:458	no free ferric ions in SPIC	440:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	8	43	theme	stronger	1218:1225	arg1	effects					1227:1233	stronger effects	1218:1233	stronger effects	1218:1233	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	3	44	theme	significant	476:486	arg1	difference					488:497	no significant difference	473:497	no significant difference in total polysaccharide content	473:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	7	45	theme	scavenging	1021:1030	arg1	effects					1032:1038	hydroxyl radicals scavenging effects	1003:1038	hydroxyl radicals scavenging effects	1003:1038	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	5	46	theme	major	751:755	arg1	fucose					778:783	fucose	778:783	fucose	778:783	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	46	theme	major	751:755	arg1	monosaccharides					757:771	the major monosaccharides	747:771	the major monosaccharides	747:771	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	47	theme	molecular	810:818	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	5	48	from	SPIC	843:846	arg1	ratio					820:824	molecular ratio	810:824	molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP	810:867	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	0	49	theme	effects	51:57	arg1	study					12:16	Comparative study	0:16	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex	0:110	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	9	50	theme	Fluorescence	1244:1255	arg1	extensity					1257:1265	Fluorescence extensity	1244:1265	Fluorescence extensity of intracellular ROS	1244:1286	Fluorescence extensity of intracellular ROS showed that SP could reduce the ROS generation while SPIC enhances the effect.					
29727645	3	51	theme	iron	579:582	arg1	content					584:590	and iron content increasing	575:601	content	584:590	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	52	from	ions	455:458	arg1	SPIC					463:466	SPIC	463:466	SPIC	463:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	52	from	ions	455:458	arg1	content					523:529	total polysaccharide content	502:529	total polysaccharide content	502:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	2	53	theme	molecular	316:324	arg1	weight					326:331	molecular weight	316:331	molecular weight	316:331	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	7	54	theme	hydroxyl	1003:1010	arg1	radicals					1012:1019	hydroxyl radicals	1003:1019	hydroxyl radicals scavenging effects	1003:1038	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	5	55	from	10:3.8:3.5	829:838	arg1	SPIC					843:846	SPIC	843:846	SPIC	843:846	Gas chromatograph (GC) showed that the major monosaccharides were fucose, mucose and glucose with molecular ratio of 10:3.8:3.5 in SPIC and 10:8.4:7.5 in SP.					
29727645	4	56	theme	Fourier-transform	604:620	arg1	spectra					638:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra indicated further that iron was chelated in polysaccharide chain.					
29727645	7	57	theme	radicals	1012:1019	arg1	effects					1032:1038	hydroxyl radicals scavenging effects	1003:1038	hydroxyl radicals scavenging effects	1003:1038	Both had hydroxyl radicals scavenging effects in vitro while SPIC showed stronger effects.					
29727645	1	58	used	used	176:179	arg2	polysaccharide					122:135	Sulfated polysaccharide	113:135	Sulfated polysaccharide (SP) extracted from sea algae	113:165	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	1	58	used	used	176:179	arg2	SP					138:139	SP	138:139	SP	138:139	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	8	59	theme	control	1119:1125	arg1	cells					1146:1150	control PMA-differentiated cells	1119:1150	control PMA-differentiated cells	1119:1150	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	1	60	theme	polysaccharide	213:226	arg1	SPIC					242:245	SPIC	242:245	SPIC	242:245	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	1	60	theme	polysaccharide	213:226	arg1	complex					233:239	polysaccharide iron complex	213:239	polysaccharide iron complex (SPIC)	213:246	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	8	61	theme	PMA-differentiated	1127:1144	arg1	cells					1146:1150	control PMA-differentiated cells	1119:1150	control PMA-differentiated cells	1119:1150	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	9	62	theme	intracellular	1270:1282	arg1	ROS					1284:1286	intracellular ROS	1270:1286	intracellular ROS	1270:1286	Fluorescence extensity of intracellular ROS showed that SP could reduce the ROS generation while SPIC enhances the effect.					
29727645	3	63	from	stability	426:434	arg1	SPIC					463:466	SPIC	463:466	SPIC	463:466	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	3	63	from	stability	426:434	arg1	content					523:529	total polysaccharide content	502:529	total polysaccharide content	502:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	2	64	theme	anti-oxidative	337:350	arg1	capability					352:361	anti-oxidative capability	337:361	anti-oxidative capability	337:361	The main active components and groups, monosaccharide composition, molecular weight and anti-oxidative capability were studied comparatively.					
29727645	1	65	theme	iron	228:231	arg1	SPIC					242:245	SPIC	242:245	SPIC	242:245	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	1	65	theme	iron	228:231	arg1	complex					233:239	polysaccharide iron complex	213:239	polysaccharide iron complex (SPIC)	213:246	Sulfated polysaccharide (SP) extracted from sea algae has been used to chelate with ferric to yield polysaccharide iron complex (SPIC).					
29727645	0	66	theme	sulfated	67:74	arg1	polysaccharide					76:89	sulfated polysaccharide	67:89	sulfated polysaccharide	67:89	Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex.					
29727645	9	67	theme	ROS	1284:1286	arg1	extensity					1257:1265	Fluorescence extensity	1244:1265	Fluorescence extensity of intracellular ROS	1244:1286	Fluorescence extensity of intracellular ROS showed that SP could reduce the ROS generation while SPIC enhances the effect.					
29727645	8	68	theme	higher	1186:1191	arg1	platform					1193:1200	higher platform	1186:1200	higher platform	1186:1200	Comparing oxygen consumption with control PMA-differentiated cells, both took longer time to achieve higher platform and SPIC showed stronger effects than SP.					
29727645	3	69	theme	total	502:506	arg1	content					523:529	total polysaccharide content	502:529	total polysaccharide content	502:529	Results showed that both have good stability and no free ferric ions in SPIC, and no significant difference in total polysaccharide content while sulfate and protein contents lowering and iron content increasing.					
29727645	4	70	theme	infrared	622:629	arg1	spectra					638:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra	604:644	Fourier-transform infrared (FTIR) spectra indicated further that iron was chelated in polysaccharide chain.					
29859842	9	0	theme	examination	1306:1316	arg1	study					1318:1322	the nuclear morphological examination study	1280:1322	the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining	1280:1413	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	1	1	from	extraction	206:215	arg1	pomace					247:252	mango pomace	241:252	mango pomace	241:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	9	2	theme	calcein	1330:1336	arg1	calcein-AM					1368:1377	calcein-AM	1368:1377	calcein-AM	1368:1377	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	2	theme	calcein	1330:1336	arg1	easter					1360:1365	calcein acetyl methoxy methyl easter	1330:1365	calcein acetyl methoxy methyl easter (calcein-AM)	1330:1378	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	4	3	theme	DEAE-52	611:617	arg1	cellulose					619:627	DEAE-52 cellulose and Sephadex G-100 column chromatography	611:668	cellulose	619:627	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	0	4	theme	mango	92:96	arg1	pomace					98:103	mango pomace	92:103	mango pomace	92:103	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	9	5	dep	easter	1360:1365	arg1	staining					1406:1413	staining	1406:1413	staining	1406:1413	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	5	6	theme	monosaccharide	696:709	arg1	composition					711:721	monosaccharide composition	696:721	monosaccharide composition	696:721	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	10	7	theme	theoretical	1447:1457	arg1	research					1421:1428	This research	1416:1428	This research	1416:1428	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	10	7	theme	theoretical	1447:1457	arg1	reference					1459:1467	a theoretical reference	1445:1467	a theoretical reference for the efficient utilization of MPFs in biomedical and functional food	1445:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	5	8	theme	high	775:778	arg1	chromatography					814:827	high performance liquid gel permeation chromatography	775:827	high performance liquid gel permeation chromatography (HPGPC)	775:835	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	8	theme	high	775:778	arg1	HPGPC					830:834	HPGPC	830:834	HPGPC	830:834	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	2	9	theme	ultrasonic	335:344	arg1	power					346:350	ultrasonic power	335:350	ultrasonic power of 170 W	335:359	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	5	10	theme	performance	780:790	arg1	chromatography					814:827	high performance liquid gel permeation chromatography	775:827	high performance liquid gel permeation chromatography (HPGPC)	775:835	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	10	theme	performance	780:790	arg1	HPGPC					830:834	HPGPC	830:834	HPGPC	830:834	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	4	11	theme	polysaccharide	511:524	arg1	fractions					526:534	Three novel polysaccharide fractions	499:534	Three novel polysaccharide fractions	499:534	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	11	theme	polysaccharide	511:524	arg1	MG-3					552:555	MG-3	552:555	MG-3	552:555	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	11	theme	polysaccharide	511:524	arg1	MG-2					543:546	MG-2	543:546	MG-2	543:546	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	11	theme	polysaccharide	511:524	arg1	MG-1					537:540	MG-1	537:540	MG-1	537:540	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	7	12	theme	polysaccharide	989:1002	arg1	fractions					1004:1012	the polysaccharide fractions	985:1012	the polysaccharide fractions	985:1012	Furthermore, the anticancer activities of the polysaccharide fractions were also investigated in vitro.					
29859842	9	13	theme	propidium	1384:1392	arg1	PI					1402:1403	PI	1402:1403	PI	1402:1403	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	13	theme	propidium	1384:1392	arg1	iodide					1394:1399	propidium iodide	1384:1399	propidium iodide (PI)	1384:1404	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	5	14	theme	liquid	792:797	arg1	chromatography					814:827	high performance liquid gel permeation chromatography	775:827	high performance liquid gel permeation chromatography (HPGPC)	775:835	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	14	theme	liquid	792:797	arg1	HPGPC					830:834	HPGPC	830:834	HPGPC	830:834	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	4	15	theme	novel	505:509	arg1	fractions					526:534	Three novel polysaccharide fractions	499:534	Three novel polysaccharide fractions	499:534	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	15	theme	novel	505:509	arg1	MG-3					552:555	MG-3	552:555	MG-3	552:555	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	15	theme	novel	505:509	arg1	MG-2					543:546	MG-2	543:546	MG-2	543:546	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	4	15	theme	novel	505:509	arg1	MG-1					537:540	MG-1	537:540	MG-1	537:540	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	8	16	theme	significant	1097:1107	arg1	activities					1120:1129	significant anticancer activities	1097:1129	significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells	1097:1196	Results showed that MG-1, MG-2 and MG-3 exhibited significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells in a dose-dependent manner.					
29859842	7	17	theme	fractions	1004:1012	arg1	activities					971:980	the anticancer activities	956:980	the anticancer activities of the polysaccharide fractions	956:1012	Furthermore, the anticancer activities of the polysaccharide fractions were also investigated in vitro.					
29859842	2	18	theme	74 °C	328:332	arg1	ratio					416:420	raw material-to-water ratio	394:420	raw material-to-water ratio	394:420	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	18	theme	74 °C	328:332	arg1	time					373:376	extraction time	362:376	extraction time of 100 min	362:387	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	18	theme	74 °C	328:332	arg1	power					346:350	ultrasonic power	335:350	ultrasonic power of 170 W	335:359	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	18	theme	74 °C	328:332	arg1	temperature					313:323	extraction temperature	302:323	extraction temperature of 74 °C	302:332	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	1	19	theme	ultrasonic-assisted	186:204	arg1	extraction					206:215	ultrasonic-assisted extraction	186:215	ultrasonic-assisted extraction of polysaccharides from mango pomace	186:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	5	20	theme	gel	799:801	arg1	chromatography					814:827	high performance liquid gel permeation chromatography	775:827	high performance liquid gel permeation chromatography (HPGPC)	775:835	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	20	theme	gel	799:801	arg1	HPGPC					830:834	HPGPC	830:834	HPGPC	830:834	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	9	21	theme	methoxy	1345:1351	arg1	calcein-AM					1368:1377	calcein-AM	1368:1377	calcein-AM	1368:1377	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	21	theme	methoxy	1345:1351	arg1	easter					1360:1365	calcein acetyl methoxy methyl easter	1330:1365	calcein acetyl methoxy methyl easter (calcein-AM)	1330:1378	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	22	theme	nuclear	1284:1290	arg1	study					1318:1322	the nuclear morphological examination study	1280:1322	the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining	1280:1413	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	8	23	theme	dose-dependent	1203:1216	arg1	manner					1218:1223	a dose-dependent manner	1201:1223	a dose-dependent manner	1201:1223	Results showed that MG-1, MG-2 and MG-3 exhibited significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells in a dose-dependent manner.					
29859842	6	24	theme	1H	927:928	arg1	NMR					930:932	1H NMR	927:932	1H NMR	927:932	The characterizations of MPFs were conducted with FT-IR, 1H NMR and SEM.					
29859842	5	25	theme	molecular	675:683	arg1	weight					685:690	molecular weight	675:690	molecular weight	675:690	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	9	26	theme	acetyl	1338:1343	arg1	calcein-AM					1368:1377	calcein-AM	1368:1377	calcein-AM	1368:1377	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	26	theme	acetyl	1338:1343	arg1	easter					1360:1365	calcein acetyl methoxy methyl easter	1330:1365	calcein acetyl methoxy methyl easter (calcein-AM)	1330:1378	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	0	27	theme	Optimization	0:11	arg1	extraction					13:22	Optimization extraction	0:22	Optimization extraction	0:22	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	4	28	theme	crude	580:584	arg1	polysaccharides					586:600	the crude polysaccharides	576:600	the crude polysaccharides	576:600	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	8	29	theme	anticancer	1109:1118	arg1	activities					1120:1129	significant anticancer activities	1097:1129	significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells	1097:1196	Results showed that MG-1, MG-2 and MG-3 exhibited significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells in a dose-dependent manner.					
29859842	7	30	theme	anticancer	960:969	arg1	activities					971:980	the anticancer activities	956:980	the anticancer activities of the polysaccharide fractions	956:1012	Furthermore, the anticancer activities of the polysaccharide fractions were also investigated in vitro.					
29859842	1	31	theme	Response	106:113	arg1	methodology					123:133	Response surface methodology	106:133	Response surface methodology	106:133	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	1	32	theme	polysaccharides	220:234	arg1	extraction					206:215	ultrasonic-assisted extraction	186:215	ultrasonic-assisted extraction of polysaccharides from mango pomace	186:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	10	33	theme	biomedical	1510:1519	arg1	food					1536:1539	biomedical and functional food	1510:1539	biomedical and functional food	1510:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	1	34	theme	surface	115:121	arg1	methodology					123:133	Response surface methodology	106:133	Response surface methodology	106:133	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	2	35	theme	170 W	355:359	arg1	ratio					416:420	raw material-to-water ratio	394:420	raw material-to-water ratio	394:420	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	35	theme	170 W	355:359	arg1	time					373:376	extraction time	362:376	extraction time of 100 min	362:387	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	35	theme	170 W	355:359	arg1	power					346:350	ultrasonic power	335:350	ultrasonic power of 170 W	335:359	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	35	theme	170 W	355:359	arg1	temperature					313:323	extraction temperature	302:323	extraction temperature of 74 °C	302:332	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	0	36	from	pomace	98:103	arg1	characterization					25:40	characterization	25:40	characterization	25:40	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	0	36	from	pomace	98:103	arg1	activities					57:66	anticancer activities	46:66	anticancer activities	46:66	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	0	36	from	pomace	98:103	arg1	extraction					13:22	Optimization extraction	0:22	Optimization extraction	0:22	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	1	37	theme	mango	241:245	arg1	pomace					247:252	mango pomace	241:252	mango pomace	241:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	0	38	theme	anticancer	46:55	arg1	activities					57:66	anticancer activities	46:66	anticancer activities	46:66	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	10	39	theme	MPFs	1502:1505	arg1	utilization					1487:1497	the efficient utilization	1473:1497	the efficient utilization of MPFs in biomedical and functional food	1473:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	3	40	theme	extraction	464:473	arg1	yield					475:479	the extraction yield	460:479	the extraction yield	460:479	Under these conditions, the extraction yield was 3.71 ± 0.07%.					
29859842	3	40	theme	extraction	464:473	arg1	%					496:496	3.71 ± 0.07%	485:496	3.71 ± 0.07%	485:496	Under these conditions, the extraction yield was 3.71 ± 0.07%.					
29859842	5	41	dep	weight	685:690	arg1	The					671:673	The	671:673	The	671:673	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	2	42	theme	extraction	267:276	arg1	conditions					278:287	The Optimum extraction conditions	255:287	The Optimum extraction conditions	255:287	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	1	43	from	pomace	247:252	arg1	polysaccharides					220:234	polysaccharides	220:234	polysaccharides from mango pomace	220:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	1	43	from	pomace	247:252	arg1	extraction					206:215	ultrasonic-assisted extraction	186:215	ultrasonic-assisted extraction of polysaccharides from mango pomace	186:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	10	44	theme	efficient	1477:1485	arg1	utilization					1487:1497	the efficient utilization	1473:1497	the efficient utilization of MPFs in biomedical and functional food	1473:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	5	45	theme	HPLC	841:844	arg1	analysis					846:853	HPLC analysis	841:853	HPLC analysis	841:853	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	8	46	theme	BGC-823	1184:1190	arg1	cells					1192:1196	BGC-823 cells	1184:1196	BGC-823 cells	1184:1196	Results showed that MG-1, MG-2 and MG-3 exhibited significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells in a dose-dependent manner.					
29859842	5	47	theme	polysaccharide	726:739	arg1	MPFs					752:755	MPFs	752:755	MPFs	752:755	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	47	theme	polysaccharide	726:739	arg1	fractions					741:749	polysaccharide fractions	726:749	polysaccharide fractions (MPFs)	726:756	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	2	48	theme	Optimum	259:265	arg1	conditions					278:287	The Optimum extraction conditions	255:287	The Optimum extraction conditions	255:287	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	49	theme	100 min	381:387	arg1	ratio					416:420	raw material-to-water ratio	394:420	raw material-to-water ratio	394:420	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	49	theme	100 min	381:387	arg1	time					373:376	extraction time	362:376	extraction time of 100 min	362:387	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	49	theme	100 min	381:387	arg1	power					346:350	ultrasonic power	335:350	ultrasonic power of 170 W	335:359	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	49	theme	100 min	381:387	arg1	temperature					313:323	extraction temperature	302:323	extraction temperature of 74 °C	302:332	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	10	50	from	utilization	1487:1497	arg1	food					1536:1539	biomedical and functional food	1510:1539	biomedical and functional food	1510:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	5	51	theme	fractions	741:749	arg1	weight					685:690	molecular weight	675:690	molecular weight	675:690	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	51	theme	fractions	741:749	arg1	composition					711:721	monosaccharide composition	696:721	monosaccharide composition	696:721	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	1	52	theme	extraction	160:169	arg1	conditions					171:180	the extraction conditions	156:180	the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace	156:252	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	10	53	theme	functional	1525:1534	arg1	food					1536:1539	biomedical and functional food	1510:1539	biomedical and functional food	1510:1539	This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food.					
29859842	6	54	theme	MPFs	895:898	arg1	characterizations					874:890	The characterizations	870:890	The characterizations of MPFs	870:898	The characterizations of MPFs were conducted with FT-IR, 1H NMR and SEM.					
29859842	1	55	used	used	139:142	arg2	methodology					123:133	Response surface methodology	106:133	Response surface methodology	106:133	Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace.					
29859842	4	56	theme	G-100	642:646	arg1	chromatography					655:668	DEAE-52 cellulose and Sephadex G-100 column chromatography	611:668	chromatography	655:668	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	2	57	theme	extraction	302:311	arg1	temperature					313:323	extraction temperature	302:323	extraction temperature of 74 °C	302:332	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	2	58	theme	extraction	362:371	arg1	time					373:376	extraction time	362:376	extraction time of 100 min	362:387	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	4	59	theme	Sephadex	633:640	arg1	chromatography					655:668	DEAE-52 cellulose and Sephadex G-100 column chromatography	611:668	chromatography	655:668	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
29859842	5	60	theme	permeation	803:812	arg1	chromatography					814:827	high performance liquid gel permeation chromatography	775:827	high performance liquid gel permeation chromatography (HPGPC)	775:835	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	5	60	theme	permeation	803:812	arg1	HPGPC					830:834	HPGPC	830:834	HPGPC	830:834	The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively.					
29859842	9	61	theme	methyl	1353:1358	arg1	calcein-AM					1368:1377	calcein-AM	1368:1377	calcein-AM	1368:1377	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	9	61	theme	methyl	1353:1358	arg1	easter					1360:1365	calcein acetyl methoxy methyl easter	1330:1365	calcein acetyl methoxy methyl easter (calcein-AM)	1330:1378	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	2	62	theme	material-to-water	398:414	arg1	ratio					416:420	raw material-to-water ratio	394:420	raw material-to-water ratio	394:420	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	0	63	theme	polysaccharides	71:85	arg1	characterization					25:40	characterization	25:40	characterization	25:40	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	0	63	theme	polysaccharides	71:85	arg1	activities					57:66	anticancer activities	46:66	anticancer activities	46:66	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	0	63	theme	polysaccharides	71:85	arg1	extraction					13:22	Optimization extraction	0:22	Optimization extraction	0:22	Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace.					
29859842	9	64	theme	morphological	1292:1304	arg1	study					1318:1322	the nuclear morphological examination study	1280:1322	the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining	1280:1413	MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining.					
29859842	2	65	theme	raw	394:396	arg1	ratio					416:420	raw material-to-water ratio	394:420	raw material-to-water ratio	394:420	The Optimum extraction conditions consisted of extraction temperature of 74 °C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL.					
29859842	4	66	theme	column	648:653	arg1	chromatography					655:668	DEAE-52 cellulose and Sephadex G-100 column chromatography	611:668	chromatography	655:668	Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography.					
31743709	4	0	theme	nanoscale	882:890	arg1	properties					901:910	their nanoscale adhesion properties	876:910	their nanoscale adhesion properties with CNCs	876:920	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	1	1	theme	biofuels	254:261	arg1	source					244:249	a renewable source	232:249	a renewable source of biofuels, chemicals and materials	232:286	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	1	1	theme	biofuels	254:261	arg1	interest					220:227	growing interest	212:227	growing interest	212:227	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	5	2	theme	different	1102:1110	arg1	films					1112:1116	the different films	1098:1116	the different films	1098:1116	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	0	3	with	modulus	74:80	arg1	nanocrystals					144:155	cellulose nanocrystals	134:155	cellulose nanocrystals at the nanoscale	134:172	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	2	4	theme	added-value	361:371	arg1	potential					373:381	high added-value potential	356:381	high added-value potential in the nanocomposite field	356:408	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	3	5	from	effect	548:553	arg1	properties					600:609	the mechanical properties	585:609	the mechanical properties	585:609	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	3	5	from	effect	548:553	arg1	polymers					514:521	polymers	514:521	polymers at the nanoscale and the effect of the external parameters on the mechanical properties	514:609	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	0	6	theme	lignocellulosic	85:99	arg1	polymers					101:108	lignocellulosic polymers	85:108	lignocellulosic polymers	85:108	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	5	7	theme	CNC-functionalized	987:1004	arg1	levers					1006:1011	CNC-functionalized levers	987:1011	CNC-functionalized levers	987:1011	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	7	8	theme	lignocellulosic	1393:1407	arg1	properties					1414:1423	the lignocellulosic film properties	1389:1423	the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites	1389:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	1	9	theme	chemicals	264:272	arg1	source					244:249	a renewable source	232:249	a renewable source of biofuels, chemicals and materials	232:286	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	1	9	theme	chemicals	264:272	arg1	interest					220:227	growing interest	212:227	growing interest	212:227	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	5	10	theme	force	957:961	arg1	experiments					970:980	force volume experiments	957:980	force volume experiments	957:980	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	7	11	theme	composites	1523:1532	arg1	production					1486:1495	the production	1482:1495	the production of new highly added-value composites	1482:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	4	12	theme	adhesion	892:899	arg1	properties					901:910	their nanoscale adhesion properties	876:910	their nanoscale adhesion properties with CNCs	876:920	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	2	13	theme	Lignocellulosic	289:303	arg1	polymers					305:312	Lignocellulosic polymers	289:312	Lignocellulosic polymers	289:312	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	7	14	theme	film	1409:1412	arg1	properties					1414:1423	the lignocellulosic film properties	1389:1423	the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites	1389:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	4	15	theme	relative	696:703	arg1	humidity					705:712	the relative humidity	692:712	the relative humidity	692:712	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	5	16	theme	atomic	929:934	arg1	microscopy					942:951	atomic force microscopy	929:951	atomic force microscopy	929:951	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	4	17	theme	relative	850:857	arg1	humidity					859:866	relative humidity	850:866	relative humidity	850:866	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	3	18	from	nanoscale	530:538	arg1	polymers					514:521	polymers	514:521	polymers at the nanoscale and the effect of the external parameters on the mechanical properties	514:609	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	4	19	theme	crystalline	789:799	arg1	cellulose					801:809	crystalline cellulose	789:809	crystalline cellulose	789:809	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	0	20	theme	polymers	101:108	arg1	modulus					74:80	the Young's modulus	62:80	the Young's modulus of lignocellulosic polymers	62:108	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	0	20	theme	polymers	101:108	arg1	adhesion					120:127	their adhesion	114:127	their adhesion with cellulose nanocrystals at the nanoscale	114:172	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	0	21	from	nanoscale	164:172	arg1	nanocrystals					144:155	cellulose nanocrystals	134:155	cellulose nanocrystals at the nanoscale	134:172	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	2	22	from	potential	373:381	arg1	field					404:408	the nanocomposite field	386:408	the nanocomposite field	386:408	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	5	23	theme	increasing	1014:1023	arg1	humidity					1038:1045	the relative humidity	1025:1045	increasing the relative humidity	1014:1045	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	1	24	theme	Lignocellulosic	175:189	arg1	biomass					191:197	Lignocellulosic biomass	175:197	Lignocellulosic biomass	175:197	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	0	25	theme	force	7:11	arg1	microscopy					13:22	Atomic force microscopy	0:22	Atomic force microscopy	0:22	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	5	26	theme	adhesion	1136:1143	arg1	forces					1145:1150	their adhesion forces	1130:1150	their adhesion forces with CNCs	1130:1160	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	3	27	theme	parameters	571:580	arg1	effect					548:553	the effect	544:553	the effect of the external parameters on the mechanical properties	544:609	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	7	28	theme	new	1500:1502	arg1	composites					1523:1532	new highly added-value composites	1500:1532	new highly added-value composites	1500:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	0	29	theme	Atomic	0:5	arg1	microscopy					13:22	Atomic force microscopy	0:22	Atomic force microscopy	0:22	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	1	30	theme	materials	278:286	arg1	source					244:249	a renewable source	232:249	a renewable source of biofuels, chemicals and materials	232:286	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	1	30	theme	materials	278:286	arg1	interest					220:227	growing interest	212:227	growing interest	212:227	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	5	31	theme	volume	963:968	arg1	experiments					970:980	force volume experiments	957:980	force volume experiments	957:980	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	2	32	theme	nanocomposite	390:402	arg1	field					404:408	the nanocomposite field	386:408	the nanocomposite field	386:408	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	6	33	from	variations	1315:1324	arg1	force					1342:1346	the adhesion force	1329:1346	the adhesion force	1329:1346	In particular, CNCs more strongly interact with glucomannan and lignin than xylan, and in the case of lignin, the oxidation of the film promotes strong variations in the adhesion force.					
31743709	6	34	theme	adhesion	1333:1340	arg1	force					1342:1346	the adhesion force	1329:1346	the adhesion force	1329:1346	In particular, CNCs more strongly interact with glucomannan and lignin than xylan, and in the case of lignin, the oxidation of the film promotes strong variations in the adhesion force.					
31743709	2	35	theme	cellulose	318:326	arg1	CNCs					342:345	CNCs	342:345	CNCs	342:345	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	2	35	theme	cellulose	318:326	arg1	nanocrystals					328:339	cellulose nanocrystals	318:339	cellulose nanocrystals (CNCs)	318:346	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	3	36	theme	external	562:569	arg1	parameters					571:580	the external parameters	558:580	the external parameters	558:580	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	1	37	theme	growing	212:218	arg1	source					244:249	a renewable source	232:249	a renewable source of biofuels, chemicals and materials	232:286	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	1	37	theme	growing	212:218	arg1	interest					220:227	growing interest	212:227	growing interest	212:227	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	0	38	theme	relative	36:43	arg1	humidity					45:52	relative humidity	36:52	relative humidity	36:52	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	0	39	theme	cellulose	134:142	arg1	nanocrystals					144:155	cellulose nanocrystals	134:155	cellulose nanocrystals at the nanoscale	134:172	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	5	40	with	forces	1145:1150	arg1	CNCs					1157:1160	CNCs	1157:1160	CNCs	1157:1160	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	4	41	theme	present	655:661	arg1	study					663:667	The present study	651:667	The present study	651:667	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	7	42	from	improvement	1467:1477	arg1	production					1486:1495	the production	1482:1495	the production of new highly added-value composites	1482:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	4	43	theme	films	769:773	arg1	modulus					742:748	the reduced Young's modulus	722:748	the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin	722:840	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	7	44	from	nanoscale	1432:1440	arg1	properties					1414:1423	the lignocellulosic film properties	1389:1423	the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites	1389:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	6	45	theme	strong	1308:1313	arg1	variations					1315:1324	strong variations	1308:1324	strong variations in the adhesion force	1308:1346	In particular, CNCs more strongly interact with glucomannan and lignin than xylan, and in the case of lignin, the oxidation of the film promotes strong variations in the adhesion force.					
31743709	5	46	theme	films	1112:1116	arg1	values					1088:1093	the Young's modulus values	1068:1093	the Young's modulus values of the different films	1068:1116	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	6	47	theme	lignin	1265:1270	arg1	case					1257:1260	the case	1253:1260	the case of lignin	1253:1270	In particular, CNCs more strongly interact with glucomannan and lignin than xylan, and in the case of lignin, the oxidation of the film promotes strong variations in the adhesion force.					
31743709	2	48	theme	high	356:359	arg1	potential					373:381	high added-value potential	356:381	high added-value potential in the nanocomposite field	356:408	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31743709	5	49	theme	Young	1072:1076	arg1	values					1088:1093	the Young's modulus values	1068:1093	the Young's modulus values of the different films	1068:1116	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	3	50	theme	mechanical	589:598	arg1	properties					600:609	the mechanical properties	585:609	the mechanical properties	585:609	Among them, the interaction between polymers at the nanoscale and the effect of the external parameters on the mechanical properties have to be more precisely investigated.					
31743709	5	51	theme	force	936:940	arg1	microscopy					942:951	atomic force microscopy	929:951	atomic force microscopy	929:951	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	4	52	with	properties	901:910	arg1	CNCs					917:920	CNCs	917:920	CNCs	917:920	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	6	53	theme	film	1294:1297	arg1	oxidation					1277:1285	the oxidation	1273:1285	the oxidation of the film	1273:1297	In particular, CNCs more strongly interact with glucomannan and lignin than xylan, and in the case of lignin, the oxidation of the film promotes strong variations in the adhesion force.					
31743709	7	54	theme	Such	1349:1352	arg1	results					1354:1360	Such results	1349:1360	Such results	1349:1360	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	4	55	dep	evaluating	677:686	arg1	changes					868:874	changes	868:874	changes their nanoscale adhesion properties with CNCs	868:920	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	4	55	dep	evaluating	677:686	arg1	affects					714:720	affects	714:720	affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin	714:840	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	4	56	theme	Young	734:738	arg1	modulus					742:748	the reduced Young's modulus	722:748	the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin	722:840	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	4	57	theme	lignocellulosic	753:767	arg1	films					769:773	lignocellulosic films	753:773	lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin	753:840	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	5	58	theme	modulus	1080:1086	arg1	values					1088:1093	the Young's modulus values	1068:1093	the Young's modulus values of the different films	1068:1116	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	5	59	theme	relative	1029:1036	arg1	humidity					1038:1045	the relative humidity	1025:1045	increasing the relative humidity	1014:1045	Using atomic force microscopy and force volume experiments with CNC-functionalized levers, increasing the relative humidity is shown to decrease the Young's modulus values of the different films and promote their adhesion forces with CNCs.					
31743709	4	60	theme	reduced	726:732	arg1	modulus					742:748	the reduced Young's modulus	722:748	the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin	722:840	The present study aims at evaluating how the relative humidity affects the reduced Young's modulus of lignocellulosic films prepared with crystalline cellulose, glucomannan, xylan and lignin and how relative humidity changes their nanoscale adhesion properties with CNCs.					
31743709	0	61	with	adhesion	120:127	arg1	nanocrystals					144:155	cellulose nanocrystals	134:155	cellulose nanocrystals at the nanoscale	134:172	Atomic force microscopy reveals how relative humidity impacts the Young's modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscale.					
31743709	1	62	theme	renewable	234:242	arg1	source					244:249	a renewable source	232:249	a renewable source of biofuels, chemicals and materials	232:286	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	1	62	theme	renewable	234:242	arg1	interest					220:227	growing interest	212:227	growing interest	212:227	Lignocellulosic biomass is receiving growing interest as a renewable source of biofuels, chemicals and materials.					
31743709	7	63	theme	added-value	1511:1521	arg1	composites					1523:1532	new highly added-value composites	1500:1532	new highly added-value composites	1500:1532	Such results allow to better understand the lignocellulosic film properties at the nanoscale, which should lead to an improvement in the production of new highly added-value composites.					
31743709	2	64	theme	large-scale	452:462	arg1	applications					464:475	large-scale applications	452:475	large-scale applications	452:475	Lignocellulosic polymers and cellulose nanocrystals (CNCs) present high added-value potential in the nanocomposite field, but some issues have to be solved before large-scale applications.					
31398402	3	0	with	fraction	631:638	arg1	SAP					696:698	SAP	696:698	SAP	696:698	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	0	with	fraction	631:638	arg1	adhesion					652:659	higher adhesion	645:659	higher adhesion	645:659	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	0	with	fraction	631:638	arg1	potential					685:693	adhesion-inhibition potential	665:693	adhesion-inhibition potential	665:693	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	1	theme	rhamnogalacturonan	837:854	arg1	SAP1					865:868	SAP1	865:868	SAP1	865:868	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	1	theme	rhamnogalacturonan	837:854	arg1	regions					856:862	xylogalacturonan and rhamnogalacturonan regions	816:862	regions	856:862	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	4	2	theme	molar	1027:1031	arg1	pectins					1038:1044	high molar mass pectins	1022:1044	high molar mass pectins	1022:1044	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	5	3	theme	recognition	1172:1182	arg1	process					1184:1190	the recognition process	1168:1190	the recognition process in the adhesion process	1168:1214	The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process.					
31398402	4	4	theme	adhesion	1056:1063	arg1	capacity					1065:1072	no adhesion capacity	1053:1072	no adhesion capacity	1053:1072	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	2	5	dep	in	485:486	arg1	vitro					488:492	vitro	488:492	vitro	488:492	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	3	6	dep	SAP1	865:868	arg1	545.5 kDa					871:879	545.5 kDa	871:879	545.5 kDa	871:879	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	2	7	theme	modified	295:302	arg1	polysaccharides					317:331	modified soybean meal polysaccharides	295:331	modified soybean meal polysaccharides	295:331	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	3	8	theme	thermochemical	557:570	arg1	process					583:589	a thermochemical extraction process	555:589	a thermochemical extraction process	555:589	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	9	theme	xylogalacturonan	816:831	arg1	SAP1					865:868	SAP1	865:868	SAP1	865:868	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	9	theme	xylogalacturonan	816:831	arg1	regions					856:862	xylogalacturonan and rhamnogalacturonan regions	816:862	regions	856:862	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	10	theme	extraction	572:581	arg1	process					583:589	a thermochemical extraction process	555:589	a thermochemical extraction process	555:589	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	11	theme	polysaccharides	733:747	arg1	-linked-β-D-galactan					896:915	a (1 → 4)-linked-β-D-galactan	887:915	a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	887:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	11	theme	polysaccharides	733:747	arg1	pectin					776:781	a partially depolymerized pectin	750:781	a partially depolymerized pectin	750:781	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	11	theme	polysaccharides	733:747	arg1	types					724:728	two types	720:728	two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	720:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	4	12	theme	Salmonella	990:999	arg1	affinity					978:985	a high affinity	971:985	a high affinity of Salmonella for galactans	971:1013	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	2	13	dep	enterobacteria	362:375	arg1	Typhimurium					389:399	Salmonella Typhimurium	378:399	Salmonella Typhimurium	378:399	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	4	14	theme	high	973:976	arg1	affinity					978:985	a high affinity	971:985	a high affinity of Salmonella for galactans	971:1013	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	3	15	dep	SAP2	936:939	arg1	8.7 kDa					942:948	8.7 kDa	942:948	8.7 kDa	942:948	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	1	16	theme	promising	162:170	arg1	alternatives					172:183	promising alternatives	162:183	promising alternatives for the control and prevention of bacterial infections	162:238	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	3	17	dep	types	724:728	arg1	-linked-β-D-galactan					896:915	a (1 → 4)-linked-β-D-galactan	887:915	a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	887:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	17	dep	types	724:728	arg1	types					724:728	two types	720:728	two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	720:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	17	dep	types	724:728	arg1	pectin					776:781	a partially depolymerized pectin	750:781	a partially depolymerized pectin	750:781	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	2	18	theme	in	485:486	arg1	assays					494:499	in vitro assays	485:499	in vitro assays	485:499	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	19	theme	chemical	273:280	arg1	structure					282:290	the chemical structure	269:290	the chemical structure of modified soybean meal polysaccharides	269:331	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	20	theme	analogue	459:466	arg1	receptors					468:476	the known analogue receptors	449:476	the known analogue receptors	449:476	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	21	dep	capacity	343:350	arg1	adhere					355:360	adhere	355:360	to adhere enterobacteria (Salmonella Typhimurium)	352:400	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	21	dep	capacity	343:350	arg1	interfere					409:417	interfere	409:417	to interfere with the bacteria adhesion to the known analogue receptors	406:476	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	22	theme	known	453:457	arg1	receptors					468:476	the known analogue receptors	449:476	the known analogue receptors	449:476	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	3	23	theme	depolymerized	762:774	arg1	pectin					776:781	a partially depolymerized pectin	750:781	a partially depolymerized pectin	750:781	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	23	theme	depolymerized	762:774	arg1	types					724:728	two types	720:728	two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	720:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	6	24	theme	Other	1217:1221	arg1	structure					1240:1248	structure	1240:1248	structure	1240:1248	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	6	24	theme	Other	1217:1221	arg1	factors					1223:1229	Other factors	1217:1229	Other factors	1217:1229	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	6	24	theme	Other	1217:1221	arg1	degree					1254:1259	degree	1254:1259	degree	1254:1259	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	2	25	theme	polysaccharides	317:331	arg1	structure					282:290	the chemical structure	269:290	the chemical structure of modified soybean meal polysaccharides	269:331	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	25	theme	polysaccharides	317:331	arg1	capacity					343:350	their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors	337:476	their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors	337:476	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	3	26	theme	molar	924:928	arg1	SAP2					936:939	SAP2	936:939	SAP2	936:939	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	26	theme	molar	924:928	arg1	mass					930:933	low molar mass	920:933	low molar mass (SAP2, 8.7 kDa)	920:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	7	27	theme	meal	1359:1362	arg1	agent					1417:1421	a promising alternative agent	1393:1421	a promising alternative agent to antibiotics for the control and prevention of foodborne diseases	1393:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	27	theme	meal	1359:1362	arg1	polysaccharides					1364:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	28	dep	control	1446:1452	arg1	the					1442:1444	the	1442:1444	the	1442:1444	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	5	29	from	process	1184:1190	arg1	process					1208:1214	the adhesion process	1195:1214	the adhesion process	1195:1214	The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process.					
31398402	2	30	theme	meal	312:315	arg1	polysaccharides					317:331	modified soybean meal polysaccharides	295:331	modified soybean meal polysaccharides	295:331	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	3	31	theme	mass	930:933	arg1	-linked-β-D-galactan					896:915	a (1 → 4)-linked-β-D-galactan	887:915	a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	887:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	31	theme	mass	930:933	arg1	pectin					776:781	a partially depolymerized pectin	750:781	a partially depolymerized pectin	750:781	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	31	theme	mass	930:933	arg1	types					724:728	two types	720:728	two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	720:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	0	32	theme	meal	17:20	arg1	polysaccharide					22:35	soybean meal polysaccharide	9:35	soybean meal polysaccharide	9:35	Modified soybean meal polysaccharide with high adhesion capacity to Salmonella.					
31398402	2	33	theme	soybean	304:310	arg1	polysaccharides					317:331	modified soybean meal polysaccharides	295:331	modified soybean meal polysaccharides	295:331	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	2	34	theme	bacteria	428:435	arg1	adhesion					437:444	the bacteria adhesion	424:444	the bacteria adhesion to the known analogue receptors	424:476	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	0	35	theme	high	42:45	arg1	capacity					56:63	high adhesion capacity	42:63	high adhesion capacity to Salmonella	42:77	Modified soybean meal polysaccharide with high adhesion capacity to Salmonella.					
31398402	3	36	theme	adhesion-inhibition	665:683	arg1	potential					685:693	adhesion-inhibition potential	665:693	adhesion-inhibition potential	665:693	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	7	37	theme	Modified	1342:1349	arg1	agent					1417:1421	a promising alternative agent	1393:1421	a promising alternative agent to antibiotics for the control and prevention of foodborne diseases	1393:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	37	theme	Modified	1342:1349	arg1	polysaccharides					1364:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	3	38	theme	low	920:922	arg1	SAP2					936:939	SAP2	936:939	SAP2	936:939	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	38	theme	low	920:922	arg1	mass					930:933	low molar mass	920:933	low molar mass (SAP2, 8.7 kDa)	920:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	7	39	theme	alternative	1405:1415	arg1	agent					1417:1421	a promising alternative agent	1393:1421	a promising alternative agent to antibiotics for the control and prevention of foodborne diseases	1393:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	39	theme	alternative	1405:1415	arg1	polysaccharides					1364:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	3	40	theme	high	787:790	arg1	mass					798:801	high molar mass	787:801	high molar mass	787:801	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	6	41	theme	polymers	1286:1293	arg1	polymerization					1264:1277	polymerization	1264:1277	polymerization of the polymers	1264:1293	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	3	42	theme	higher	645:650	arg1	SAP					696:698	SAP	696:698	SAP	696:698	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	42	theme	higher	645:650	arg1	adhesion					652:659	higher adhesion	645:659	higher adhesion	645:659	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	7	43	theme	soybean	1351:1357	arg1	agent					1417:1421	a promising alternative agent	1393:1421	a promising alternative agent to antibiotics for the control and prevention of foodborne diseases	1393:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	43	theme	soybean	1351:1357	arg1	polysaccharides					1364:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	3	44	theme	molar	792:796	arg1	mass					798:801	high molar mass	787:801	high molar mass	787:801	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	6	45	theme	adhesion	1324:1331	arg1	process					1333:1339	the adhesion process	1320:1339	the adhesion process	1320:1339	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	0	46	theme	adhesion	47:54	arg1	capacity					56:63	high adhesion capacity	42:63	high adhesion capacity to Salmonella	42:77	Modified soybean meal polysaccharide with high adhesion capacity to Salmonella.					
31398402	7	47	theme	diseases	1482:1489	arg1	control					1446:1452	control	1446:1452	control	1446:1452	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	47	theme	diseases	1482:1489	arg1	prevention					1458:1467	prevention	1458:1467	prevention	1458:1467	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	3	48	theme	structural	595:604	arg1	analyses					606:613	structural analyses	595:613	structural analyses	595:613	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	5	49	theme	fractions	1108:1116	arg1	compositions					1088:1099	The chemical compositions	1075:1099	The chemical compositions of the fractions	1075:1116	The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process.					
31398402	1	50	theme	analog	114:119	arg1	receptors					121:129	analog receptors	114:129	analog receptors for bacteria	114:142	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	1	50	theme	analog	114:119	arg1	Carbohydrates					80:92	Carbohydrates	80:92	Carbohydrates	80:92	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	5	51	theme	adhesion	1199:1206	arg1	process					1208:1214	the adhesion process	1195:1214	the adhesion process	1195:1214	The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process.					
31398402	6	52	theme	polymerization	1264:1277	arg1	structure					1240:1248	structure	1240:1248	structure	1240:1248	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	6	52	theme	polymerization	1264:1277	arg1	degree					1254:1259	degree	1254:1259	degree	1254:1259	Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process.					
31398402	3	53	dep	-linked-β-D-galactan	896:915	arg1	1 → 4					890:894	1 → 4	890:894	1 → 4	890:894	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	4	54	theme	mass	1033:1036	arg1	pectins					1038:1044	high molar mass pectins	1022:1044	high molar mass pectins	1022:1044	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	1	55	theme	bacterial	219:227	arg1	infections					229:238	bacterial infections	219:238	bacterial infections	219:238	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	7	56	theme	promising	1395:1403	arg1	agent					1417:1421	a promising alternative agent	1393:1421	a promising alternative agent to antibiotics for the control and prevention of foodborne diseases	1393:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	7	56	theme	promising	1395:1403	arg1	polysaccharides					1364:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides	1342:1378	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31398402	3	57	theme	meal	520:523	arg1	suspensions					525:535	soybean meal suspensions	512:535	soybean meal suspensions	512:535	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	1	58	theme	infections	229:238	arg1	prevention					205:214	prevention	205:214	prevention	205:214	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	1	58	theme	infections	229:238	arg1	control					193:199	control	193:199	control	193:199	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	4	59	theme	high	1022:1025	arg1	pectins					1038:1044	high molar mass pectins	1022:1044	high molar mass pectins	1022:1044	The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity.					
31398402	1	60	dep	control	193:199	arg1	the					189:191	the	189:191	the	189:191	Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections.					
31398402	3	61	theme	soybean	512:518	arg1	suspensions					525:535	soybean meal suspensions	512:535	soybean meal suspensions	512:535	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	62	theme	mass	798:801	arg1	-linked-β-D-galactan					896:915	a (1 → 4)-linked-β-D-galactan	887:915	a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	887:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	62	theme	mass	798:801	arg1	pectin					776:781	a partially depolymerized pectin	750:781	a partially depolymerized pectin	750:781	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	3	62	theme	mass	798:801	arg1	types					724:728	two types	720:728	two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa)	720:949	For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-β-D-galactan of low molar mass (SAP2, 8.7 kDa).					
31398402	5	63	theme	chemical	1079:1086	arg1	compositions					1088:1099	The chemical compositions	1075:1099	The chemical compositions of the fractions	1075:1116	The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process.					
31398402	2	64	theme	present	245:251	arg1	study					253:257	The present study	241:257	The present study	241:257	The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays.					
31398402	0	65	theme	soybean	9:15	arg1	polysaccharide					22:35	soybean meal polysaccharide	9:35	soybean meal polysaccharide	9:35	Modified soybean meal polysaccharide with high adhesion capacity to Salmonella.					
31398402	7	66	theme	foodborne	1472:1480	arg1	diseases					1482:1489	foodborne diseases	1472:1489	foodborne diseases	1472:1489	Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.					
31678104	3	0	theme	water	689:693	arg1	extraction					695:704	water extraction	689:704	water extraction	689:704	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	8	1	contain	had	1505:1507	arg1	SCP-2					1499:1503	SCP-2	1499:1503	SCP-2	1499:1503	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	1	contain	had	1505:1507	arg2	activity					1530:1537	stronger antioxidant activity	1509:1537	stronger antioxidant activity	1509:1537	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	1	2	from	constricta	274:283	arg1	polysaccharides					240:254	polysaccharides	240:254	polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction	240:313	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	3	theme	structural	332:341	arg1	characteristics					343:357	structural characteristics	332:357	structural characteristics	332:357	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	8	4	dep	activity	1349:1356	arg1	the					1330:1332	the	1330:1332	the	1330:1332	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	4	dep	activity	1349:1356	arg1	activity					1451:1458	inhibition activity	1440:1458	inhibition activity	1440:1458	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	4	5	theme	fraction	891:898	arg1	composition					868:878	the composition	864:878	the composition of the SCP fraction	864:898	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	8	6	theme	SCP-2	1473:1477	arg1	activity					1349:1356	Fe2+ chelating activity	1334:1356	Fe2+ chelating activity	1334:1356	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	6	theme	SCP-2	1473:1477	arg1	activity					1407:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	6	theme	SCP-2	1473:1477	arg1	peroxidation					1427:1438	lipid peroxidation	1421:1438	lipid peroxidation	1421:1438	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	2	7	theme	content	631:637	arg1	%					642:642	enzyme content 4.0%	624:642	enzyme content 4.0%	624:642	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	6	8	theme	uronic	1160:1165	arg1	acids					1167:1171	uronic acids	1160:1171	uronic acids	1160:1171	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	8	9	theme	Fe2+	1334:1337	arg1	activity					1349:1356	Fe2+ chelating activity	1334:1356	Fe2+ chelating activity	1334:1356	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	1	10	dep	fractions	421:429	arg1	SCP-2					442:446	SCP-2	442:446	SCP-2	442:446	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	10	dep	fractions	421:429	arg1	SCP-1					432:436	SCP-1	432:436	SCP-1	432:436	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	10	dep	fractions	421:429	arg1	fractions					421:429	the two purified polysaccharide fractions	389:429	the two purified polysaccharide fractions (SCP-1 and SCP-2)	389:447	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	4	11	theme	polysaccharide	909:922	arg1	content					924:930	the polysaccharide content	905:930	the polysaccharide content produced by enzymatic extraction	905:963	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	4	11	theme	polysaccharide	909:922	arg1	higher					968:973	higher	968:973	higher	968:973	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	2	12	theme	enzyme	624:629	arg1	%					642:642	enzyme content 4.0%	624:642	enzyme content 4.0%	624:642	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	0	13	theme	structural	107:116	arg1	activities					149:158	their structural characteristic and antioxidant activities	101:158	their structural characteristic and antioxidant activities	101:158	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	1	14	theme	antioxidant	363:373	arg1	activities					375:384	antioxidant activities	363:384	antioxidant activities	363:384	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	2	15	theme	temperature	601:611	arg1	50.0 ℃					613:618	enzymolysis temperature 50.0 ℃	589:618	enzymolysis temperature 50.0 ℃	589:618	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	6	16	theme	sulfuric	1138:1145	arg1	radicals					1147:1154	sulfuric radicals	1138:1154	sulfuric radicals	1138:1154	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	1	17	theme	paper	177:181	arg1	aim					165:167	The aim	161:167	The aim of this paper	161:181	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	0	18	theme	mild	2:5	arg1	method					32:37	A mild and efficient extraction method	0:37	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.	0:159	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	2	19	theme	enzymolysis	589:599	arg1	50.0 ℃					613:618	enzymolysis temperature 50.0 ℃	589:618	enzymolysis temperature 50.0 ℃	589:618	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	7	20	theme	molecular	1274:1282	arg1	weights					1284:1290	their molecular weights	1268:1290	their molecular weights	1268:1290	Both SCP-1 and SCP-2 were mainly consisted of glucose, but their molecular weights were different.					
31678104	3	21	theme	enzymatic	664:672	arg1	extraction					674:683	enzymatic extraction	664:683	enzymatic extraction	664:683	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	3	22	theme	UV-visible	732:741	arg1	images					759:764	visual, UV-visible and IR spectrum images	724:764	visual, UV-visible and IR spectrum images	724:764	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	8	23	theme	inhibition	1440:1449	arg1	activity					1451:1458	inhibition activity	1440:1458	inhibition activity	1440:1458	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	5	24	dep	properties	1002:1011	arg1	the					982:984	the	982:984	the	982:984	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	0	25	theme	efficient	11:19	arg1	method					32:37	A mild and efficient extraction method	0:37	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.	0:159	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	1	26	dep	characteristics	343:357	arg1	the					328:330	the	328:330	the	328:330	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	5	27	theme	structural	1017:1026	arg1	characteristics					1028:1042	structural characteristics	1017:1042	structural characteristics	1017:1042	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	6	28	theme	carbohydrate	1116:1127	arg1	content					1129:1135	The carbohydrate content	1112:1135	The carbohydrate content	1112:1135	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	3	29	theme	visual	724:729	arg1	images					759:764	visual, UV-visible and IR spectrum images	724:764	visual, UV-visible and IR spectrum images	724:764	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	0	30	theme	characteristic	118:131	arg1	activities					149:158	their structural characteristic and antioxidant activities	101:158	their structural characteristic and antioxidant activities	101:158	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	5	31	theme	SCP-2	1057:1061	arg1	properties					1002:1011	physicochemical properties	986:1011	physicochemical properties	986:1011	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	5	31	theme	SCP-2	1057:1061	arg1	characteristics					1028:1042	structural characteristics	1017:1042	structural characteristics	1017:1042	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	2	32	theme	extraction	466:475	arg1	conditions					477:486	enzyme extraction conditions	459:486	enzyme extraction conditions	459:486	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	8	33	theme	antioxidant	1518:1528	arg1	activity					1530:1537	stronger antioxidant activity	1509:1537	stronger antioxidant activity	1509:1537	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	34	theme	stronger	1509:1516	arg1	activity					1530:1537	stronger antioxidant activity	1509:1537	stronger antioxidant activity	1509:1537	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	0	35	theme	activities	149:158	arg1	constricta					77:86	Sinonovacula constricta	64:86	Sinonovacula constricta	64:86	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	0	35	theme	activities	149:158	arg1	study					92:96	study	92:96	study	92:96	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	0	36	from	constricta	77:86	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities	43:158	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	5	37	theme	physicochemical	986:1000	arg1	properties					1002:1011	physicochemical properties	986:1011	physicochemical properties	986:1011	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	1	38	theme	mild	199:202	arg1	method					229:234	a mild and efficient extraction method	197:234	a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction	197:313	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	0	39	theme	extraction	21:30	arg1	method					32:37	A mild and efficient extraction method	0:37	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.	0:159	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	1	40	theme	enzyme	297:302	arg1	extraction					304:313	enzyme extraction	297:313	enzyme extraction	297:313	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	0	41	theme	antioxidant	137:147	arg1	activities					149:158	their structural characteristic and antioxidant activities	101:158	their structural characteristic and antioxidant activities	101:158	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	1	42	theme	purified	397:404	arg1	SCP-2					442:446	SCP-2	442:446	SCP-2	442:446	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	42	theme	purified	397:404	arg1	SCP-1					432:436	SCP-1	432:436	SCP-1	432:436	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	42	theme	purified	397:404	arg1	fractions					421:429	the two purified polysaccharide fractions	389:429	the two purified polysaccharide fractions (SCP-1 and SCP-2)	389:447	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	8	43	dep	turned	1483:1488	arg1	out					1490:1492	out	1490:1492	out	1490:1492	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	2	44	theme	enzyme	459:464	arg1	conditions					477:486	enzyme extraction conditions	459:486	enzyme extraction conditions	459:486	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	2	45	theme	time	565:568	arg1	173.0 min					570:578	enzymolysis time 173.0 min	553:578	enzymolysis time 173.0 min	553:578	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	1	46	theme	polysaccharide	406:419	arg1	SCP-2					442:446	SCP-2	442:446	SCP-2	442:446	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	46	theme	polysaccharide	406:419	arg1	SCP-1					432:436	SCP-1	432:436	SCP-1	432:436	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	46	theme	polysaccharide	406:419	arg1	fractions					421:429	the two purified polysaccharide fractions	389:429	the two purified polysaccharide fractions (SCP-1 and SCP-2)	389:447	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	8	47	theme	radical	1365:1371	arg1	activity					1407:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	3	48	theme	IR	747:748	arg1	images					759:764	visual, UV-visible and IR spectrum images	724:764	visual, UV-visible and IR spectrum images	724:764	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	2	49	theme	enzymolysis	553:563	arg1	173.0 min					570:578	enzymolysis time 173.0 min	553:578	enzymolysis time 173.0 min	553:578	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	1	50	theme	fractions	421:429	arg1	characteristics					343:357	structural characteristics	332:357	structural characteristics	332:357	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	1	50	theme	fractions	421:429	arg1	activities					375:384	antioxidant activities	363:384	antioxidant activities	363:384	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	4	51	theme	significant	813:823	arg1	difference					825:834	no significant difference	810:834	no significant difference between them with regard to the composition of the SCP fraction	810:898	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	8	52	theme	lipid	1421:1425	arg1	peroxidation					1427:1438	lipid peroxidation	1421:1438	lipid peroxidation	1421:1438	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	6	53	theme	fractions	1184:1192	arg1	content					1129:1135	The carbohydrate content	1112:1135	The carbohydrate content	1112:1135	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	6	53	theme	fractions	1184:1192	arg1	radicals					1147:1154	sulfuric radicals	1138:1154	sulfuric radicals	1138:1154	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	6	53	theme	fractions	1184:1192	arg1	acids					1167:1171	uronic acids	1160:1171	uronic acids	1160:1171	The carbohydrate content, sulfuric radicals and uronic acids of the two fractions were detected.					
31678104	3	54	theme	spectrum	750:757	arg1	images					759:764	visual, UV-visible and IR spectrum images	724:764	visual, UV-visible and IR spectrum images	724:764	Comparison between enzymatic extraction and water extraction was obtained from visual, UV-visible and IR spectrum images.					
31678104	4	55	theme	SCP	887:889	arg1	fraction					891:898	the SCP fraction	883:898	the SCP fraction	883:898	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	8	56	theme	superoxide	1377:1386	arg1	radical					1388:1394	superoxide radical	1377:1394	superoxide radical	1377:1394	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	57	theme	chelating	1339:1347	arg1	activity					1349:1356	Fe2+ chelating activity	1334:1356	Fe2+ chelating activity	1334:1356	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	1	58	theme	efficient	208:216	arg1	method					229:234	a mild and efficient extraction method	197:234	a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction	197:313	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	5	59	theme	SCP-1	1047:1051	arg1	properties					1002:1011	physicochemical properties	986:1011	physicochemical properties	986:1011	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	5	59	theme	SCP-1	1047:1051	arg1	characteristics					1028:1042	structural characteristics	1017:1042	structural characteristics	1017:1042	Then, the physicochemical properties and structural characteristics of SCP-1 and SCP-2 were investigated using FT-TR, UV, GC and HPGPC.					
31678104	8	60	theme	SCP-1	1463:1467	arg1	activity					1349:1356	Fe2+ chelating activity	1334:1356	Fe2+ chelating activity	1334:1356	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	60	theme	SCP-1	1463:1467	arg1	activity					1407:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	60	theme	SCP-1	1463:1467	arg1	peroxidation					1427:1438	lipid peroxidation	1421:1438	lipid peroxidation	1421:1438	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	1	61	theme	extraction	218:227	arg1	method					229:234	a mild and efficient extraction method	197:234	a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction	197:313	The aim of this paper is to develop a mild and efficient extraction method for polysaccharides from Sinonovacula constricta (SCP) using enzyme extraction, and analyze the structural characteristics and antioxidant activities of the two purified polysaccharide fractions (SCP-1 and SCP-2).					
31678104	0	62	theme	Sinonovacula	64:75	arg1	constricta					77:86	Sinonovacula constricta	64:86	Sinonovacula constricta	64:86	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	0	63	from	study	92:96	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities	43:158	A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities.					
31678104	8	64	theme	scavenging	1396:1405	arg1	activity					1407:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	8	65	theme	ABTS+	1359:1363	arg1	radical					1365:1371	ABTS+ radical	1359:1371	ABTS+ radical	1359:1371	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
31678104	4	66	theme	enzymatic	944:952	arg1	extraction					954:963	enzymatic extraction	944:963	enzymatic extraction	944:963	The results clearly indicate that there is no significant difference between them with regard to the composition of the SCP fraction, but the polysaccharide content produced by enzymatic extraction is higher.					
31678104	2	67	dep	follows	544:550	arg1	pH					581:582	pH 8.2	581:586	pH 8.2	581:586	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	2	67	dep	follows	544:550	arg1	173.0 min					570:578	enzymolysis time 173.0 min	553:578	enzymolysis time 173.0 min	553:578	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	2	67	dep	follows	544:550	arg1	%					642:642	enzyme content 4.0%	624:642	enzyme content 4.0%	624:642	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	2	67	dep	follows	544:550	arg1	50.0 ℃					613:618	enzymolysis temperature 50.0 ℃	589:618	enzymolysis temperature 50.0 ℃	589:618	Firstly, enzyme extraction conditions were optimized, and the conditions were found to be, as follows: enzymolysis time 173.0 min, pH 8.2, enzymolysis temperature 50.0 ℃ and enzyme content 4.0%.					
31678104	8	68	theme	radical	1388:1394	arg1	activity					1407:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	ABTS+ radical and superoxide radical scavenging activity	1359:1414	In addition, compared the Fe2+ chelating activity, ABTS+ radical and superoxide radical scavenging activity, and lipid peroxidation inhibition activity of SCP-1 and SCP-2, it turned out that SCP-2 had stronger antioxidant activity than SCP-1.					
30451447	2	0	theme	NSP	461:463	arg1	sources					450:456	richest sources	442:456	richest sources of NSP in grains	442:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	0	1	from	Potential	16:24	arg1	Avena					67:71	Avena	67:71	Avena	67:71	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	0	1	from	Potential	16:24	arg1	Oats					61:64	Oats	61:64	Oats (Avena sativa L.)	61:82	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	1	2	from	influence	294:302	arg1	health					348:353	metabolic health	338:353	metabolic health	338:353	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	2	from	influence	294:302	arg1	host					307:310	host intestinal microbiota and metabolic health	307:353	host	307:310	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	3	theme	hemicellulose	1097:1109	arg1	easier					1111:1116	hemicellulose easier	1097:1116	hemicellulose easier to access for other bacteria	1097:1145	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	0	4	from	Oats	61:64	arg1	Potential					16:24	Broad Prebiotic Potential	0:24	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	0	4	from	Oats	61:64	arg1	Polysaccharides					40:54	Non-starch Polysaccharides	29:54	Non-starch Polysaccharides from Oats (Avena sativa L.)	29:82	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	1	5	theme	intestinal	312:321	arg1	microbiota					323:332	intestinal microbiota	312:332	intestinal microbiota	312:332	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	5	6	theme	new	758:760	arg1	candidates					762:771	new candidates	758:771	new candidates for the syntrophic bacterial interactions	758:813	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	4	7	theme	prebiotics	675:684	arg1	fermentation					659:670	fermentation	659:670	fermentation of prebiotics	659:684	Moreover, bacterial cross-feeding interactions in which fermentation of prebiotics is involved is poorly characterized.					
30451447	7	8	theme	succinic	1210:1217	arg1	acids					1219:1223	lactic and succinic acids	1199:1223	lactic and succinic acids	1199:1223	Because of fermentation, B. licheniformis produces lactic and succinic acids, which further can be used by other bacteria for cross-feeding and SCFA production.					
30451447	0	9	dep	in	88:89	arg1	vitro					91:95	vitro	91:95	vitro	91:95	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	1	10	theme	metabolic	338:346	arg1	health					348:353	metabolic health	338:353	metabolic health	338:353	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	0	11	theme	in	88:89	arg1	Study					97:101	an in vitro Study	85:101	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	6	12	theme	oat	1074:1076	arg1	β-glucan					1078:1085	oat β-glucan	1074:1085	oat β-glucan	1074:1085	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	5	13	theme	polysaccharides	852:866	arg1	fermentability					819:832	fermentability	819:832	fermentability of oat non-starch polysaccharides (NSP)	819:872	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	5	13	theme	polysaccharides	852:866	arg1	exploration					743:753	the exploration	739:753	the exploration of new candidates for the syntrophic bacterial interactions	739:813	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	2	14	from	sources	450:456	arg1	grains					468:473	grains	468:473	grains	468:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	2	15	theme	sources	450:456	arg1	one					435:437	one	435:437	one	435:437	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	2	15	theme	sources	450:456	arg1	sources					450:456	richest sources	442:456	richest sources of NSP in grains	442:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	5	16	theme	bacterial	792:800	arg1	interactions					802:813	the syntrophic bacterial interactions	777:813	the syntrophic bacterial interactions	777:813	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	4	17	theme	cross-feeding	623:635	arg1	interactions					637:648	bacterial cross-feeding interactions	613:648	bacterial cross-feeding interactions in which fermentation of prebiotics is involved	613:696	Moreover, bacterial cross-feeding interactions in which fermentation of prebiotics is involved is poorly characterized.					
30451447	0	18	theme	Prebiotic	6:14	arg1	Potential					16:24	Broad Prebiotic Potential	0:24	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	6	19	from	composition	915:925	arg1	medium					984:989	fermentation medium	971:989	fermentation medium	971:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	7	20	theme	SCFA	1292:1295	arg1	production					1297:1306	SCFA production	1292:1306	SCFA production	1292:1306	Because of fermentation, B. licheniformis produces lactic and succinic acids, which further can be used by other bacteria for cross-feeding and SCFA production.					
30451447	1	21	dep	growth	128:133	arg1	the					124:126	the	124:126	the	124:126	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	22	from	viscosity	928:936	arg1	medium					984:989	fermentation medium	971:989	fermentation medium	971:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	1	23	theme	beneficial	150:159	arg1	bacteria					172:179	beneficial intestinal bacteria	150:179	beneficial intestinal bacteria	150:179	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	0	24	theme	Broad	0:4	arg1	Potential					16:24	Broad Prebiotic Potential	0:24	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	6	25	theme	fermentation	971:982	arg1	medium					984:989	fermentation medium	971:989	fermentation medium	971:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	1	26	theme	intestinal	161:170	arg1	bacteria					172:179	beneficial intestinal bacteria	150:179	beneficial intestinal bacteria	150:179	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	27	theme	wide	256:259	arg1	recognition					261:271	wide recognition	256:271	wide recognition	256:271	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	28	theme	NSP	964:966	arg1	concentration					942:954	concentration	942:954	concentration	942:954	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	28	theme	NSP	964:966	arg1	viscosity					928:936	viscosity	928:936	viscosity	928:936	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	28	theme	NSP	964:966	arg1	composition					915:925	composition	915:925	composition	915:925	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	0	29	dep	Avena	67:71	arg1	L.					80:81	Avena sativa L.	67:81	Avena sativa L.	67:81	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	1	30	theme	bacteria	172:179	arg1	activity					138:145	activity	138:145	activity	138:145	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	30	theme	bacteria	172:179	arg1	growth					128:133	growth	128:133	growth	128:133	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	31	dep	host	307:310	arg1	microbiota					323:332	intestinal microbiota	312:332	intestinal microbiota	312:332	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	2	32	from	NSP	461:463	arg1	grains					468:473	grains	468:473	grains	468:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	6	33	theme	high	1056:1059	arg1	viscosity					1061:1069	high viscosity	1056:1069	high viscosity of oat β-glucan	1056:1085	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	0	34	theme	Polysaccharides	40:54	arg1	Potential					16:24	Broad Prebiotic Potential	0:24	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	6	35	theme	oats	959:962	arg1	NSP					964:966	oats NSP	959:966	oats NSP in fermentation medium	959:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	2	36	theme	prebiotic	512:520	arg1	effect					522:527	prebiotic effect	512:527	prebiotic effect	512:527	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	5	37	theme	oat	837:839	arg1	NSP					869:871	NSP	869:871	NSP	869:871	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	5	37	theme	oat	837:839	arg1	polysaccharides					852:866	oat non-starch polysaccharides	837:866	oat non-starch polysaccharides (NSP)	837:872	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	3	38	theme	specific	570:577	arg1	NSP					579:581	specific NSP	570:581	specific NSP of oats	570:589	However, information on fermentation of specific NSP of oats is limited.					
30451447	2	39	theme	non-starch	361:370	arg1	NSP					389:391	NSP	389:391	NSP	389:391	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	2	39	theme	non-starch	361:370	arg1	polysaccharides					372:386	Some non-starch polysaccharides	356:386	Some non-starch polysaccharides (NSP)	356:392	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	4	40	theme	bacterial	613:621	arg1	interactions					637:648	bacterial cross-feeding interactions	613:648	bacterial cross-feeding interactions in which fermentation of prebiotics is involved	613:696	Moreover, bacterial cross-feeding interactions in which fermentation of prebiotics is involved is poorly characterized.					
30451447	0	41	theme	Non-starch	29:38	arg1	Polysaccharides					40:54	Non-starch Polysaccharides	29:54	Non-starch Polysaccharides from Oats (Avena sativa L.)	29:82	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	2	42	theme	richest	442:448	arg1	sources					450:456	richest sources	442:456	richest sources of NSP in grains	442:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	2	43	from	grains	468:473	arg1	sources					450:456	richest sources	442:456	richest sources of NSP in grains	442:473	Some non-starch polysaccharides (NSP) are defined as prebiotics and oats being one of richest sources of NSP in grains are considered as potentially having prebiotic effect.					
30451447	3	44	theme	NSP	579:581	arg1	fermentation					554:565	fermentation	554:565	fermentation of specific NSP of oats	554:589	However, information on fermentation of specific NSP of oats is limited.					
30451447	5	45	theme	non-starch	841:850	arg1	NSP					869:871	NSP	869:871	NSP	869:871	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	5	45	theme	non-starch	841:850	arg1	polysaccharides					852:866	oat non-starch polysaccharides	837:866	oat non-starch polysaccharides (NSP)	837:872	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	1	46	theme	beneficial	283:292	arg1	influence					294:302	their beneficial influence	277:302	their beneficial influence on host intestinal microbiota and metabolic health	277:353	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	47	dep	NSP	1042:1044	arg1	makes					1091:1095	makes	1091:1095	makes hemicellulose easier to access for other bacteria	1091:1145	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	47	dep	NSP	1042:1044	arg1	degrades					1047:1054	degrades	1047:1054	degrades high viscosity of oat β-glucan	1047:1085	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	48	from	NSP	964:966	arg1	medium					984:989	fermentation medium	971:989	fermentation medium	971:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	49	from	medium	984:989	arg1	concentration					942:954	concentration	942:954	concentration	942:954	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	49	from	medium	984:989	arg1	viscosity					928:936	viscosity	928:936	viscosity	928:936	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	49	from	medium	984:989	arg1	composition					915:925	composition	915:925	composition	915:925	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	6	50	theme	other	1132:1136	arg1	bacteria					1138:1145	other bacteria	1132:1145	other bacteria	1132:1145	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	7	51	used	used	1247:1250	arg2	acids					1219:1223	lactic and succinic acids	1199:1223	lactic and succinic acids	1199:1223	Because of fermentation, B. licheniformis produces lactic and succinic acids, which further can be used by other bacteria for cross-feeding and SCFA production.					
30451447	5	52	theme	candidates	762:771	arg1	fermentability					819:832	fermentability	819:832	fermentability of oat non-starch polysaccharides (NSP)	819:872	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	5	52	theme	candidates	762:771	arg1	exploration					743:753	the exploration	739:753	the exploration of new candidates for the syntrophic bacterial interactions	739:813	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30451447	6	53	theme	oat	1038:1040	arg1	NSP					1042:1044	oat NSP	1038:1044	oat NSP	1038:1044	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	1	54	theme	short-chain	204:214	arg1	acids					222:226	short-chain fatty acids	204:226	short-chain fatty acids (SCFA)	204:233	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	54	theme	short-chain	204:214	arg1	SCFA					229:232	SCFA	229:232	SCFA	229:232	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	55	from	concentration	942:954	arg1	medium					984:989	fermentation medium	971:989	fermentation medium	971:989	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	0	56	dep	Potential	16:24	arg1	Study					97:101	an in vitro Study	85:101	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.	0:102	Broad Prebiotic Potential of Non-starch Polysaccharides from Oats (Avena sativa L.): an in vitro Study.					
30451447	7	57	theme	other	1255:1259	arg1	bacteria					1261:1268	other bacteria	1255:1268	other bacteria	1255:1268	Because of fermentation, B. licheniformis produces lactic and succinic acids, which further can be used by other bacteria for cross-feeding and SCFA production.					
30451447	3	58	from	information	539:549	arg1	fermentation					554:565	fermentation	554:565	fermentation of specific NSP of oats	554:589	However, information on fermentation of specific NSP of oats is limited.					
30451447	1	59	theme	fatty	216:220	arg1	acids					222:226	short-chain fatty acids	204:226	short-chain fatty acids (SCFA)	204:233	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	1	59	theme	fatty	216:220	arg1	SCFA					229:232	SCFA	229:232	SCFA	229:232	Prebiotics inducing the growth or activity of beneficial intestinal bacteria - probiotics producing short-chain fatty acids (SCFA) have lately received wide recognition for their beneficial influence on host intestinal microbiota and metabolic health.					
30451447	6	60	theme	β-glucan	1078:1085	arg1	viscosity					1061:1069	high viscosity	1056:1069	high viscosity of oat β-glucan	1056:1085	The results obtained by differentiating composition, viscosity and concentration of oats NSP in fermentation medium showed that Bacillus licheniformis pre-digests oat NSP, degrades high viscosity of oat β-glucan and makes hemicellulose easier to access for other bacteria.					
30451447	7	61	theme	lactic	1199:1204	arg1	acids					1219:1223	lactic and succinic acids	1199:1223	lactic and succinic acids	1199:1223	Because of fermentation, B. licheniformis produces lactic and succinic acids, which further can be used by other bacteria for cross-feeding and SCFA production.					
30451447	3	62	theme	oats	586:589	arg1	NSP					579:581	specific NSP	570:581	specific NSP of oats	570:589	However, information on fermentation of specific NSP of oats is limited.					
30451447	5	63	theme	syntrophic	781:790	arg1	interactions					802:813	the syntrophic bacterial interactions	777:813	the syntrophic bacterial interactions	777:813	Here, we report the exploration of new candidates for the syntrophic bacterial interactions and fermentability of oat non-starch polysaccharides (NSP).					
30589960	2	0	theme	yam	365:367	arg1	polysaccharide					369:382	yam polysaccharide	365:382	yam polysaccharide	365:382	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	6	1	theme	colitic	1254:1260	arg1	rats					1262:1265	colitic rats	1254:1265	colitic rats	1254:1265	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	3	2	theme	METHODS	558:564	arg1	rats					574:577	METHODS Male SD rats	558:577	METHODS Male SD rats	558:577	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	1	3	theme	Inflammatory	131:142	arg1	disease					150:156	AIM Inflammatory bowel disease	127:156	AIM Inflammatory bowel disease	127:156	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	0	4	theme	Compound	0:7	arg1	polysaccharides					9:23	Compound polysaccharides	0:23	Compound polysaccharides	0:23	Compound polysaccharides ameliorate experimental colitis by modulating gut microbiota composition and function.					
30589960	5	5	theme	reduced	824:830	arg1	inflammation					832:843	CP reduced inflammation	821:843	CP reduced inflammation in the rat model of colitis induced by TNBS	821:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	6	6	theme	Bacteroides	1130:1140	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	6	theme	Bacteroides	1130:1140	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	7	theme	dysregulated	1218:1229	arg1	function					1242:1249	the dysregulated microbiota function	1214:1249	the dysregulated microbiota function of colitic rats	1214:1265	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	2	8	theme	compound	324:331	arg1	CP					350:351	CP	350:351	CP	350:351	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	8	theme	compound	324:331	arg1	polysaccharides					333:347	compound polysaccharides	324:347	compound polysaccharides (CP) containing yam polysaccharide and inulin	324:393	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	1	9	theme	microbial	216:224	arg1	flora					226:230	intestinal microbial flora	205:230	intestinal microbial flora in individuals with genetic predisposition(s)	205:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	3	10	theme	SD	571:572	arg1	rats					574:577	METHODS Male SD rats	558:577	METHODS Male SD rats	558:577	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	2	11	theme	function	548:555	arg1	terms					508:512	terms	508:512	terms of gut microbiota composition and function	508:555	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	1	12	from	flora	226:230	arg1	individuals					235:245	individuals	235:245	individuals with genetic predisposition(s)	235:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	1	13	theme	bowel	144:148	arg1	disease					150:156	AIM Inflammatory bowel disease	127:156	AIM Inflammatory bowel disease	127:156	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	7	14	theme	colitis	1735:1741	arg1	individuals					1743:1753	colitis individuals	1735:1753	colitis individuals	1735:1753	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	7	15	theme	microbiota	1608:1617	arg1	composition					1619:1629	gut microbiota composition	1604:1629	gut microbiota composition	1604:1629	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	4	16	theme	myeloperoxidase	695:709	arg1	level					711:715	serum myeloperoxidase level	689:715	serum myeloperoxidase level	689:715	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	6	17	theme	normal	1274:1279	arg1	condition					1281:1289	a normal condition	1272:1289	a normal condition	1272:1289	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	5	18	from	inflammation	832:843	arg1	model					856:860	the rat model	848:860	the rat model of colitis induced by TNBS	848:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	6	19	theme	sulfate-reducing	1169:1184	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	19	theme	sulfate-reducing	1169:1184	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	0	20	theme	microbiota	75:84	arg1	composition					86:96	gut microbiota composition	71:96	gut microbiota composition	71:96	Compound polysaccharides ameliorate experimental colitis by modulating gut microbiota composition and function.					
30589960	5	21	theme	rat	852:854	arg1	model					856:860	the rat model	848:860	the rat model of colitis induced by TNBS	848:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	4	22	theme	activity	673:680	arg1	index					682:686	Disease activity index	665:686	Disease activity index	665:686	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	7	23	used	used	1684:1687	arg2	agents					1702:1707	prebiotic agents	1692:1707	prebiotic agents	1692:1707	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	7	23	used	used	1684:1687	arg2	it					1666:1667	it	1666:1667	it	1666:1667	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	1	24	theme	immune	186:191	arg1	response					193:200	a dysregulated immune response	171:200	a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s)	171:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	2	25	theme	composition	532:542	arg1	terms					508:512	terms	508:512	terms of gut microbiota composition and function	508:555	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	5	26	theme	experimental	909:920	arg1	colitis					922:928	the experimental colitis	905:928	the experimental colitis	905:928	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	7	27	theme	rats	1565:1568	arg1	colitis					1554:1560	the experimental colitis	1537:1560	the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals	1537:1753	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	2	28	theme	gut	517:519	arg1	composition					532:542	gut microbiota composition	517:542	gut microbiota composition	517:542	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	6	29	from	reduction	1343:1351	arg1	metabolism					1442:1451	metabolism	1442:1451	metabolism	1442:1451	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	29	from	reduction	1343:1351	arg1	stress					1366:1371	oxidative stress	1356:1371	oxidative stress	1356:1371	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	29	from	reduction	1343:1351	arg1	transduction					1396:1407	signal transduction	1389:1407	signal transduction	1389:1407	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	29	from	reduction	1343:1351	arg1	metabolism					1326:1335	basic metabolism	1320:1335	basic metabolism	1320:1335	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	29	from	reduction	1343:1351	arg1	biodegradation					1422:1435	xenobiotics biodegradation	1410:1435	xenobiotics biodegradation	1410:1435	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	29	from	reduction	1343:1351	arg1	motility					1379:1386	cell motility	1374:1386	cell motility	1374:1386	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	30	theme	xenobiotics	1410:1420	arg1	biodegradation					1422:1435	xenobiotics biodegradation	1410:1435	xenobiotics biodegradation	1410:1435	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	metabolism					1442:1451	metabolism	1442:1451	metabolism	1442:1451	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	stress					1366:1371	oxidative stress	1356:1371	oxidative stress	1356:1371	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	transduction					1396:1407	signal transduction	1389:1407	signal transduction	1389:1407	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	metabolism					1326:1335	basic metabolism	1320:1335	basic metabolism	1320:1335	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	biodegradation					1422:1435	xenobiotics biodegradation	1410:1435	xenobiotics biodegradation	1410:1435	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	31	from	improvement	1305:1315	arg1	motility					1379:1386	cell motility	1374:1386	cell motility	1374:1386	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	32	theme	short-chain	1032:1042	arg1	SCFAs					1057:1061	SCFAs	1057:1061	SCFAs	1057:1061	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	32	theme	short-chain	1032:1042	arg1	acids					1050:1054	increased short-chain fatty acids	1022:1054	increased short-chain fatty acids (SCFAs)	1022:1062	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	7	33	theme	experimental	1541:1552	arg1	colitis					1554:1560	the experimental colitis	1537:1560	the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals	1537:1753	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	1	34	theme	AIM	127:129	arg1	disease					150:156	AIM Inflammatory bowel disease	127:156	AIM Inflammatory bowel disease	127:156	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	2	35	theme	colitis	415:421	arg1	model					406:410	the rat model	398:410	the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS)	398:474	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	6	36	theme	gut	997:999	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	36	theme	gut	997:999	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	37	theme	cell	1374:1377	arg1	motility					1379:1386	cell motility	1374:1386	cell motility	1374:1386	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	7	38	theme	Compound	1500:1507	arg1	polysaccharides					1509:1523	CONCLUSIONS Compound polysaccharides	1488:1523	CONCLUSIONS Compound polysaccharides	1488:1523	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	6	39	theme	acid-producing	1091:1104	arg1	bacteria					1106:1113	lactic acid-producing bacteria	1084:1113	lactic acid-producing bacteria	1084:1113	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	3	40	theme	CP	655:656	arg1	group					658:662	the CP group	651:662	the CP group	651:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	40	theme	CP	655:656	arg1	groups					603:608	three groups	597:608	three groups: the control group, the model group, and the CP group	597:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	4	41	theme	microbiota	758:767	arg1	level					711:715	serum myeloperoxidase level	689:715	serum myeloperoxidase level	689:715	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	4	41	theme	microbiota	758:767	arg1	function					742:749	function	742:749	function	742:749	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	4	41	theme	microbiota	758:767	arg1	composition					726:736	composition	726:736	composition	726:736	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	4	41	theme	microbiota	758:767	arg1	index					682:686	Disease activity index	665:686	Disease activity index	665:686	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	6	42	theme	pathogenesis	1464:1475	arg1	processes					1477:1485	pathogenesis processes	1464:1485	pathogenesis processes	1464:1485	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	1	43	theme	genetic	252:258	arg1	s					275:275	s	275:275	s	275:275	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	1	43	theme	genetic	252:258	arg1	predisposition					260:273	genetic predisposition	252:273	genetic predisposition(s)	252:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	6	44	theme	-producing	1063:1072	arg1	bacteria					1074:1081	dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria	1001:1081	dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria	1001:1081	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	45	theme	rats	1262:1265	arg1	function					1242:1249	the dysregulated microbiota function	1214:1249	the dysregulated microbiota function of colitic rats	1214:1265	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	7	46	from	dysbiosis	1722:1730	arg1	individuals					1743:1753	colitis individuals	1735:1753	colitis individuals	1735:1753	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	5	47	dep	RESULTS	784:790	arg1	showed					814:819	showed	814:819	showed CP reduced inflammation in the rat model of colitis induced by TNBS	814:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	5	47	dep	RESULTS	784:790	arg1	ameliorated					893:903	ameliorated	893:903	ameliorated the experimental colitis	893:928	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	7	48	theme	gut	1718:1720	arg1	dysbiosis					1722:1730	gut dysbiosis	1718:1730	gut dysbiosis in colitis individuals	1718:1753	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	5	49	theme	CP	821:822	arg1	inflammation					832:843	CP reduced inflammation	821:843	CP reduced inflammation in the rat model of colitis induced by TNBS	821:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	6	50	theme	microbiota	1231:1240	arg1	function					1242:1249	the dysregulated microbiota function	1214:1249	the dysregulated microbiota function of colitic rats	1214:1265	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	2	51	theme	polysaccharides	333:347	arg1	effects					313:319	the effects	309:319	the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS)	309:474	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	1	52	theme	intestinal	205:214	arg1	flora					226:230	intestinal microbial flora	205:230	intestinal microbial flora in individuals with genetic predisposition(s)	205:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	6	53	theme	decreased	1120:1128	arg1	Bacteroides					1130:1140	decreased Bacteroides	1120:1140	decreased Bacteroides	1120:1140	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	54	theme	basic	1320:1324	arg1	metabolism					1326:1335	basic metabolism	1320:1335	basic metabolism	1320:1335	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	3	55	theme	Male	566:569	arg1	rats					574:577	METHODS Male SD rats	558:577	METHODS Male SD rats	558:577	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	7	56	theme	function	1635:1642	arg1	profiles					1644:1651	the gut microbiota composition and function profiles	1600:1651	the gut microbiota composition and function profiles	1600:1651	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	2	57	from	mechanism	495:503	arg1	terms					508:512	terms	508:512	terms of gut microbiota composition and function	508:555	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	4	58	dep	composition	726:736	arg1	the					722:724	the	722:724	the	722:724	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	3	59	dep	groups	603:608	arg1	groups					603:608	three groups	597:608	three groups: the control group, the model group, and the CP group	597:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	59	dep	groups	603:608	arg1	group					658:662	the CP group	651:662	the CP group	651:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	59	dep	groups	603:608	arg1	group					640:644	the model group	630:644	the model group	630:644	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	59	dep	groups	603:608	arg1	group					623:627	the control group	611:627	the control group	611:627	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	7	60	theme	composition	1619:1629	arg1	profiles					1644:1651	the gut microbiota composition and function profiles	1600:1651	the gut microbiota composition and function profiles	1600:1651	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	5	61	theme	colitis	865:871	arg1	model					856:860	the rat model	848:860	the rat model of colitis induced by TNBS	848:887	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	7	62	theme	gut	1604:1606	arg1	composition					1619:1629	gut microbiota composition	1604:1629	gut microbiota composition	1604:1629	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	3	63	theme	control	615:621	arg1	groups					603:608	three groups	597:608	three groups: the control group, the model group, and the CP group	597:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	63	theme	control	615:621	arg1	group					623:627	the control group	611:627	the control group	611:627	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	6	64	theme	TNBS-induced	984:995	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	64	theme	TNBS-induced	984:995	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	4	65	theme	serum	689:693	arg1	level					711:715	serum myeloperoxidase level	689:715	serum myeloperoxidase level	689:715	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	6	66	theme	oxidative	1356:1364	arg1	stress					1366:1371	oxidative stress	1356:1371	oxidative stress	1356:1371	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	1	67	theme	dysregulated	173:184	arg1	response					193:200	a dysregulated immune response	171:200	a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s)	171:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	2	68	from	effects	313:319	arg1	model					406:410	the rat model	398:410	the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS)	398:474	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	4	69	theme	Disease	665:671	arg1	index					682:686	Disease activity index	665:686	Disease activity index	665:686	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	2	70	contain	containing	354:363	arg2	inulin					388:393	inulin	388:393	inulin	388:393	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	70	contain	containing	354:363	arg2	polysaccharide					369:382	yam polysaccharide	365:382	yam polysaccharide	365:382	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	70	contain	containing	354:363	arg1	CP					350:351	CP	350:351	CP	350:351	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	70	contain	containing	354:363	arg1	polysaccharides					333:347	compound polysaccharides	324:347	compound polysaccharides (CP) containing yam polysaccharide and inulin	324:393	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	71	theme	microbiota	521:530	arg1	composition					532:542	gut microbiota composition	517:542	gut microbiota composition	517:542	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	5	72	from	data	796:799	arg1	study					808:812	the study	804:812	the study	804:812	RESULTS The data in the study showed CP reduced inflammation in the rat model of colitis induced by TNBS and ameliorated the experimental colitis.					
30589960	7	73	theme	prebiotic	1692:1700	arg1	agents					1702:1707	prebiotic agents	1692:1707	prebiotic agents	1692:1707	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	7	73	theme	prebiotic	1692:1700	arg1	it					1666:1667	it	1666:1667	it	1666:1667	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	2	74	theme	rat	402:404	arg1	model					406:410	the rat model	398:410	the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS)	398:474	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	6	75	theme	fatty	1044:1048	arg1	SCFAs					1057:1061	SCFAs	1057:1061	SCFAs	1057:1061	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	75	theme	fatty	1044:1048	arg1	acids					1050:1054	increased short-chain fatty acids	1022:1054	increased short-chain fatty acids (SCFAs)	1022:1062	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	0	76	theme	experimental	36:47	arg1	colitis					49:55	experimental colitis	36:55	experimental colitis	36:55	Compound polysaccharides ameliorate experimental colitis by modulating gut microbiota composition and function.					
30589960	1	77	with	individuals	235:245	arg1	s					275:275	s	275:275	s	275:275	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	1	77	with	individuals	235:245	arg1	predisposition					260:273	genetic predisposition	252:273	genetic predisposition(s)	252:276	BACKGROUND AND AIM Inflammatory bowel disease results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s).					
30589960	6	78	theme	increased	1022:1030	arg1	SCFAs					1057:1061	SCFAs	1057:1061	SCFAs	1057:1061	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	78	theme	increased	1022:1030	arg1	acids					1050:1054	increased short-chain fatty acids	1022:1054	increased short-chain fatty acids (SCFAs)	1022:1062	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	2	79	theme	2,4,6-trinitrobenzenesulfonic	434:462	arg1	TNBS					470:473	TNBS	470:473	TNBS	470:473	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	2	79	theme	2,4,6-trinitrobenzenesulfonic	434:462	arg1	acid					464:467	2,4,6-trinitrobenzenesulfonic acid	434:467	2,4,6-trinitrobenzenesulfonic acid (TNBS)	434:474	This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function.					
30589960	3	80	theme	model	634:638	arg1	groups					603:608	three groups	597:608	three groups: the control group, the model group, and the CP group	597:662	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	3	80	theme	model	634:638	arg1	group					640:644	the model group	630:644	the model group	630:644	METHODS Male SD rats were divided into three groups: the control group, the model group, and the CP group.					
30589960	6	81	theme	signal	1389:1394	arg1	transduction					1396:1407	signal transduction	1389:1407	signal transduction	1389:1407	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	82	theme	dysbiosis-indexed	1001:1017	arg1	bacteria					1074:1081	dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria	1001:1081	dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria	1001:1081	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	0	83	theme	gut	71:73	arg1	composition					86:96	gut microbiota composition	71:96	gut microbiota composition	71:96	Compound polysaccharides ameliorate experimental colitis by modulating gut microbiota composition and function.					
30589960	6	84	theme	bacteria	1106:1113	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	84	theme	bacteria	1106:1113	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	85	theme	lactic	1084:1089	arg1	bacteria					1106:1113	lactic acid-producing bacteria	1084:1113	lactic acid-producing bacteria	1084:1113	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	4	86	theme	gut	754:756	arg1	microbiota					758:767	gut microbiota	754:767	gut microbiota	754:767	Disease activity index, serum myeloperoxidase level, and the composition and function of gut microbiota were analyzed.					
30589960	7	87	theme	CONCLUSIONS	1488:1498	arg1	polysaccharides					1509:1523	CONCLUSIONS Compound polysaccharides	1488:1523	CONCLUSIONS Compound polysaccharides	1488:1523	CONCLUSIONS Compound polysaccharides ameliorated the experimental colitis of rats induced by TNBS by modulating the gut microbiota composition and function profiles, which makes it possible to be used as prebiotic agents to treat gut dysbiosis in colitis individuals.					
30589960	6	88	theme	bacteria	1074:1081	arg1	bacteria					1186:1193	sulfate-reducing bacteria	1169:1193	sulfate-reducing bacteria	1169:1193	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30589960	6	88	theme	bacteria	1074:1081	arg1	Proteobacteria					1143:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria	984:1156	The results also indicated that CP not only reversed TNBS-induced gut dysbiosis-indexed by increased short-chain fatty acids (SCFAs)-producing bacteria, lactic acid-producing bacteria, and decreased Bacteroides, Proteobacteria as well as sulfate-reducing bacteria, but also restored the dysregulated microbiota function of colitic rats into a normal condition, including an improvement on basic metabolism and a reduction on oxidative stress, cell motility, signal transduction, xenobiotics biodegradation, and metabolism as well as pathogenesis processes.					
30513259	4	0	theme	CH2	832:834	arg1	orientations					843:854	different CH2 domain orientations	822:854	different CH2 domain orientations	822:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	8	1	theme	refined	1574:1580	arg1	variants					1596:1603	highly refined functional Fc variants	1567:1603	highly refined functional Fc variants	1567:1603	These findings will guide the development of methodology for the production of highly refined functional Fc variants.					
30513259	7	2	from	orientation	1463:1473	arg1	solution					1478:1485	solution	1478:1485	solution	1478:1485	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	7	3	theme	glycan	1416:1421	arg1	removal					1392:1398	the removal	1388:1398	the removal of the N-linked glycan	1388:1421	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	8	4	theme	Fc	1593:1594	arg1	variants					1596:1603	highly refined functional Fc variants	1567:1603	highly refined functional Fc variants	1567:1603	These findings will guide the development of methodology for the production of highly refined functional Fc variants.					
30513259	3	5	theme	X-ray	518:522	arg1	SAXS					536:539	SAXS	536:539	SAXS	536:539	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	5	theme	X-ray	518:522	arg1	scattering					524:533	small-angle X-ray scattering	506:533	the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution	502:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	1	6	theme	fragment	270:277	arg1	region					284:289	the crystallizable fragment (Fc) region	251:289	the crystallizable fragment (Fc) region	251:289	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	4	7	theme	Fc	807:808	arg1	region					810:815	the Fc region	803:815	the Fc region with different CH2 domain orientations	803:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	2	8	link	N-linked	355:362	arg1	glycan					364:369	the N-linked glycan	351:369	the N-linked glycan	351:369	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	6	9	theme	best-fitted	1202:1212	arg1	profiles					1219:1226	the best-fitted SAXS profiles	1198:1226	the best-fitted SAXS profiles	1198:1226	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	1	10	theme	immunoglobulin	188:201	arg1	G					203:203	immunoglobulin G	188:203	immunoglobulin G	188:203	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	3	11	gly	glycosylated	557:568	arg1	region					573:578	the glycosylated Fc region	553:578	the glycosylated Fc region (gFc)	553:584	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	11	gly	glycosylated	557:568	arg1	gFc					581:583	gFc	581:583	gFc	581:583	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	4	12	with	comparison	712:721	arg1	profiles					733:740	SAXS profiles	728:740	SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations	728:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	0	13	theme	Fc	118:119	arg1	regions					121:127	aglycosylated Fc regions	104:127	aglycosylated Fc regions	104:127	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	6	14	theme	crystal	1273:1279	arg1	structure					1281:1289	the crystal structure	1269:1289	the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation	1269:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	5	15	theme	first	1028:1032	arg1	component					1044:1052	the first principal component	1024:1052	the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis	1024:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	3	16	theme	glycosylated	557:568	arg1	region					573:578	the glycosylated Fc region	553:578	the glycosylated Fc region (gFc)	553:584	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	16	theme	glycosylated	557:568	arg1	gFc					581:583	gFc	581:583	gFc	581:583	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	1	17	dep	stability	225:233	arg1	the					221:223	the	221:223	the	221:223	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	0	18	theme	X-ray	147:151	arg1	scattering					153:162	small-angle X-ray scattering	135:162	small-angle X-ray scattering	135:162	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	19	theme	structure	994:1002	arg1	openness					958:965	openness	958:965	openness	958:965	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	5	19	theme	structure	994:1002	arg1	closure					970:976	closure	970:976	closure	970:976	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	4	20	theme	CH2	671:673	arg1	orientation					682:692	the CH2 domain orientation	667:692	the CH2 domain orientation	667:692	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	5	21	theme	fitting	896:902	arg1	analyses					904:911	the fitting analyses	892:911	the fitting analyses of gFc and aFc	892:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	6	22	theme	CH2	1326:1328	arg1	domain					1330:1335	a "semi-closed" CH2 domain	1310:1335	a "semi-closed" CH2 domain orientation	1310:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	3	23	theme	region	573:578	arg1	profile					542:548	the small-angle X-ray scattering (SAXS) profile	502:548	the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution	502:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	0	24	from	orientation	11:21	arg1	solution					52:59	solution	52:59	solution	52:59	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	4	25	theme	Conformational	633:646	arg1	characteristics					648:662	Conformational characteristics	633:662	Conformational characteristics of the CH2 domain orientation	633:692	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	0	26	theme	Structural	62:71	arg1	comparison					73:82	Structural comparison	62:82	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	27	theme	chi-square	869:878	arg1	values					880:885	The reduced chi-square values	857:885	The reduced chi-square values from the fitting analyses of gFc and aFc	857:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	4	28	theme	SAXS	728:731	arg1	profiles					733:740	SAXS profiles	728:740	SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations	728:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	2	29	theme	domain	425:430	arg1	orientation					432:442	the CH2 domain orientation	417:442	the CH2 domain orientation	417:442	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	1	30	theme	region	284:289	arg1	function					239:246	function	239:246	function	239:246	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	1	30	theme	region	284:289	arg1	stability					225:233	stability	225:233	stability	225:233	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	1	31	link	N-linked	169:176	arg1	critical					208:215	critical	208:215	critical	208:215	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	1	31	link	N-linked	169:176	arg1	glycan					178:183	The N-linked glycan	165:183	The N-linked glycan in immunoglobulin G	165:203	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	4	32	theme	orientation	682:692	arg1	characteristics					648:662	Conformational characteristics	633:662	Conformational characteristics of the CH2 domain orientation	633:692	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	0	33	theme	CH2	0:2	arg1	orientation					11:21	CH2 domain orientation	0:21	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	34	theme	singular	1137:1144	arg1	decomposition					1152:1164	a singular value decomposition	1135:1164	a singular value decomposition analysis	1135:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	0	35	gly	aglycosylated	104:116	arg1	regions					121:127	aglycosylated Fc regions	104:127	aglycosylated Fc regions	104:127	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	2	36	theme	Fc	451:452	arg1	region					454:459	the Fc region	447:459	the Fc region	447:459	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	3	37	theme	Fc	604:605	arg1	aFc					615:617	aFc	615:617	aFc	615:617	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	37	theme	Fc	604:605	arg1	region					607:612	aglycosylated Fc region	590:612	aglycosylated Fc region (aFc)	590:618	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	4	38	theme	crystal	781:787	arg1	structures					789:798	multiple crystal structures	772:798	multiple crystal structures of the Fc region with different CH2 domain orientations	772:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	0	39	theme	human	26:30	arg1	G					47:47	human immunoglobulin G	26:47	human immunoglobulin G	26:47	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	40	theme	CH2	1099:1101	arg1	domain					1103:1108	the CH2 domain	1095:1108	the CH2 domain orientation extracted by a singular value decomposition analysis	1095:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	0	41	theme	G	47:47	arg1	orientation					11:21	CH2 domain orientation	0:21	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	42	theme	orientation	1110:1120	arg1	variation					1082:1090	the variation	1078:1090	the variation of the CH2 domain orientation extracted by a singular value decomposition analysis	1078:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	7	43	theme	CH2	1452:1454	arg1	orientation					1463:1473	the CH2 domain orientation	1448:1473	the CH2 domain orientation in solution	1448:1485	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	5	44	theme	crystal	986:992	arg1	structure					994:1002	each crystal structure	981:1002	each crystal structure	981:1002	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	2	45	theme	properties	320:329	arg1	Alteration					292:301	Alteration	292:301	Alteration of these protein properties upon the removal of the N-linked glycan	292:369	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	8	46	theme	variants	1596:1603	arg1	production					1553:1562	the production	1549:1562	the production of highly refined functional Fc variants	1549:1603	These findings will guide the development of methodology for the production of highly refined functional Fc variants.					
30513259	0	47	dep	orientation	11:21	arg1	comparison					73:82	Structural comparison	62:82	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	2	48	theme	N-linked	355:362	arg1	glycan					364:369	the N-linked glycan	351:369	the N-linked glycan	351:369	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	2	49	from	alteration	403:412	arg1	region					454:459	the Fc region	447:459	the Fc region	447:459	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	5	50	theme	decomposition	1152:1164	arg1	analysis					1166:1173	a singular value decomposition analysis	1135:1173	a singular value decomposition analysis	1135:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	7	51	theme	N-linked	1407:1414	arg1	glycan					1416:1421	the N-linked glycan	1403:1421	the N-linked glycan	1403:1421	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	4	52	theme	different	822:830	arg1	orientations					843:854	different CH2 domain orientations	822:854	different CH2 domain orientations	822:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	3	53	gly	aglycosylated	590:602	arg1	aFc					615:617	aFc	615:617	aFc	615:617	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	53	gly	aglycosylated	590:602	arg1	region					607:612	aglycosylated Fc region	590:612	aglycosylated Fc region (aFc)	590:618	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	54	theme	small-angle	506:516	arg1	SAXS					536:539	SAXS	536:539	SAXS	536:539	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	54	theme	small-angle	506:516	arg1	scattering					524:533	small-angle X-ray scattering	506:533	the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution	502:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	1	55	theme	crystallizable	255:268	arg1	region					284:289	the crystallizable fragment (Fc) region	251:289	the crystallizable fragment (Fc) region	251:289	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	4	56	theme	domain	836:841	arg1	orientations					843:854	different CH2 domain orientations	822:854	different CH2 domain orientations	822:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	8	57	theme	functional	1582:1591	arg1	variants					1596:1603	highly refined functional Fc variants	1567:1603	highly refined functional Fc variants	1567:1603	These findings will guide the development of methodology for the production of highly refined functional Fc variants.					
30513259	6	58	theme	SAXS	1214:1217	arg1	profiles					1219:1226	the best-fitted SAXS profiles	1198:1226	the best-fitted SAXS profiles	1198:1226	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	1	59	theme	N-linked	169:176	arg1	critical					208:215	critical	208:215	critical	208:215	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	1	59	theme	N-linked	169:176	arg1	glycan					178:183	The N-linked glycan	165:183	The N-linked glycan in immunoglobulin G	165:203	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	6	60	theme	semi-closed	1313:1323	arg1	domain					1330:1335	a "semi-closed" CH2 domain	1310:1335	a "semi-closed" CH2 domain orientation	1310:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	4	61	theme	region	810:815	arg1	structures					789:798	multiple crystal structures	772:798	multiple crystal structures of the Fc region with different CH2 domain orientations	772:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	7	62	link	N-linked	1407:1414	arg1	glycan					1416:1421	the N-linked glycan	1403:1421	the N-linked glycan	1403:1421	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	6	63	theme	gFc	1294:1296	arg1	structure					1281:1289	the crystal structure	1269:1289	the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation	1269:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	3	64	theme	scattering	524:533	arg1	profile					542:548	the small-angle X-ray scattering (SAXS) profile	502:548	the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution	502:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	0	65	theme	small-angle	135:145	arg1	scattering					153:162	small-angle X-ray scattering	135:162	small-angle X-ray scattering	135:162	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	66	theme	principal	1034:1042	arg1	component					1044:1052	the first principal component	1024:1052	the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis	1024:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	0	67	theme	regions	121:127	arg1	comparison					73:82	Structural comparison	62:82	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	6	68	theme	domain	1330:1335	arg1	orientation					1337:1347	a "semi-closed" CH2 domain orientation	1310:1347	a "semi-closed" CH2 domain orientation	1310:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	3	69	theme	Fc	570:571	arg1	region					573:578	the glycosylated Fc region	553:578	the glycosylated Fc region (gFc)	553:584	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	69	theme	Fc	570:571	arg1	gFc					581:583	gFc	581:583	gFc	581:583	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	6	70	theme	"	1324:1324	arg1	domain					1330:1335	a "semi-closed" CH2 domain	1310:1335	a "semi-closed" CH2 domain orientation	1310:1347	For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a "semi-closed" CH2 domain orientation.					
30513259	1	71	from	glycan	178:183	arg1	G					203:203	immunoglobulin G	188:203	immunoglobulin G	188:203	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	5	72	theme	reduced	861:867	arg1	values					880:885	The reduced chi-square values	857:885	The reduced chi-square values from the fitting analyses of gFc and aFc	857:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	4	73	with	region	810:815	arg1	orientations					843:854	different CH2 domain orientations	822:854	different CH2 domain orientations	822:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	0	74	theme	glycosylated	87:98	arg1	comparison					73:82	Structural comparison	62:82	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	75	theme	aFc	924:926	arg1	analyses					904:911	the fitting analyses	892:911	the fitting analyses of gFc and aFc	892:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	0	76	theme	aglycosylated	104:116	arg1	regions					121:127	aglycosylated Fc regions	104:127	aglycosylated Fc regions	104:127	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	0	77	theme	domain	4:9	arg1	orientation					11:21	CH2 domain orientation	0:21	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.	0:163	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	2	78	theme	CH2	421:423	arg1	orientation					432:442	the CH2 domain orientation	417:442	the CH2 domain orientation	417:442	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	4	79	theme	domain	675:680	arg1	orientation					682:692	the CH2 domain orientation	667:692	the CH2 domain orientation	667:692	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	3	80	from	profile	542:548	arg1	solution					623:630	solution	623:630	solution	623:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	2	81	theme	glycan	364:369	arg1	removal					340:346	the removal	336:346	the removal of the N-linked glycan	336:369	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	1	82	theme	Fc	280:281	arg1	region					284:289	the crystallizable fragment (Fc) region	251:289	the crystallizable fragment (Fc) region	251:289	The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region.					
30513259	5	83	theme	gFc	916:918	arg1	analyses					904:911	the fitting analyses	892:911	the fitting analyses of gFc and aFc	892:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	3	84	theme	aglycosylated	590:602	arg1	aFc					615:617	aFc	615:617	aFc	615:617	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	3	84	theme	aglycosylated	590:602	arg1	region					607:612	aglycosylated Fc region	590:612	aglycosylated Fc region (aFc)	590:618	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	0	85	theme	immunoglobulin	32:45	arg1	G					47:47	human immunoglobulin G	26:47	human immunoglobulin G	26:47	CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.					
30513259	5	86	theme	value	1146:1150	arg1	decomposition					1152:1164	a singular value decomposition	1135:1164	a singular value decomposition analysis	1135:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	5	87	theme	closure	970:976	arg1	degree					948:953	the degree	944:953	the degree of openness or closure of each crystal structure	944:1002	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	4	88	theme	multiple	772:779	arg1	structures					789:798	multiple crystal structures	772:798	multiple crystal structures of the Fc region with different CH2 domain orientations	772:854	Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations.					
30513259	5	89	theme	domain	1103:1108	arg1	orientation					1110:1120	the CH2 domain orientation	1095:1120	the CH2 domain orientation extracted by a singular value decomposition analysis	1095:1173	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	2	90	theme	orientation	432:442	arg1	alteration					403:412	the alteration	399:412	the alteration of the CH2 domain orientation in the Fc region	399:459	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	8	91	theme	methodology	1533:1543	arg1	development					1518:1528	the development	1514:1528	the development of methodology for the production of highly refined functional Fc variants	1514:1603	These findings will guide the development of methodology for the production of highly refined functional Fc variants.					
30513259	7	92	theme	domain	1456:1461	arg1	orientation					1463:1473	the CH2 domain orientation	1448:1473	the CH2 domain orientation in solution	1448:1485	Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution.					
30513259	3	93	theme	region	607:612	arg1	profile					542:548	the small-angle X-ray scattering (SAXS) profile	502:548	the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution	502:630	To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution.					
30513259	2	94	theme	protein	312:318	arg1	properties					320:329	these protein properties	306:329	these protein properties	306:329	Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region.					
30513259	5	95	from	analyses	904:911	arg1	values					880:885	The reduced chi-square values	857:885	The reduced chi-square values from the fitting analyses of gFc and aFc	857:926	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30513259	5	96	theme	openness	958:965	arg1	degree					948:953	the degree	944:953	the degree of openness or closure of each crystal structure	944:1002	The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis.					
30267825	0	0	theme	antioxidant	87:97	arg1	evaluation					116:125	their antioxidant and antidiabetic evaluation	81:125	their antioxidant and antidiabetic evaluation	81:125	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	1	1	theme	various	163:169	arg1	activities					182:191	various biological activities	163:191	various biological activities	163:191	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	8	2	theme	oxidative	1336:1344	arg1	damages					1346:1352	oxidative damages	1336:1352	oxidative damages	1336:1352	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	4	3	dep	GSH	811:813	arg1	p < 0.05					816:823	p < 0.05	816:823	p < 0.05	816:823	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	3	dep	GSH	811:813	arg1	p < 0.01					826:833	p < 0.01	826:833	p < 0.01	826:833	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	6	4	theme	evident	944:950	arg1	improvement					952:962	an evident improvement	941:962	an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05)	941:1032	Furthermore, PPe-H could cause an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).					
30267825	1	5	theme	biological	171:180	arg1	activities					182:191	various biological activities	163:191	various biological activities	163:191	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	4	6	from	loss	652:655	arg1	rats					665:668	T2DM rats	660:668	T2DM rats	660:668	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	0	7	theme	antidiabetic	103:114	arg1	evaluation					116:125	their antioxidant and antidiabetic evaluation	81:125	their antioxidant and antidiabetic evaluation	81:125	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	0	8	from	Preparation	0:10	arg1	polysaccharides					61:75	pumpkin polysaccharides	53:75	pumpkin polysaccharides	53:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	7	9	dep	both	1054:1057	arg1	heteropolysaccharide					1063:1082	the heteropolysaccharide	1059:1082	the heteropolysaccharide	1059:1082	PPe and PPe-H were both the heteropolysaccharide and composed of rhamnose, arabinose, glucose and galactose, their molecular weight were 104.27 kDa and 37.58 kDa, respectively.					
30267825	8	10	theme	GLP-1	1318:1322	arg1	secretion					1294:1302	the secretion	1290:1302	the secretion of endogenous GLP-1	1290:1322	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	2	11	theme	central	295:301	arg1	design					313:318	central composite design	295:318	central composite design (CCD)	295:324	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	2	11	theme	central	295:301	arg1	CCD					321:323	CCD	321:323	CCD	321:323	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	4	12	theme	level	802:806	arg1	activities					723:732	the activities	719:732	the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01)	719:834	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	8	13	theme	antidiabetic	1226:1237	arg1	related					1267:1273	related	1267:1273	related	1267:1273	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	8	13	theme	antidiabetic	1226:1237	arg1	mechanism					1239:1247	The potential antidiabetic mechanism	1212:1247	The potential antidiabetic mechanism of PPe-H	1212:1256	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	4	14	theme	T2DM	660:663	arg1	rats					665:668	T2DM rats	660:668	T2DM rats	660:668	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	7	15	theme	molecular	1150:1158	arg1	weight					1160:1165	their molecular weight	1144:1165	their molecular weight	1144:1165	PPe and PPe-H were both the heteropolysaccharide and composed of rhamnose, arabinose, glucose and galactose, their molecular weight were 104.27 kDa and 37.58 kDa, respectively.					
30267825	8	16	theme	PPe-H	1252:1256	arg1	related					1267:1273	related	1267:1273	related	1267:1273	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	8	16	theme	PPe-H	1252:1256	arg1	mechanism					1239:1247	The potential antidiabetic mechanism	1212:1247	The potential antidiabetic mechanism of PPe-H	1212:1256	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	6	17	theme	stimulated	975:984	arg1	secretion					992:1000	glucose stimulated GLP-1 secretion	967:1000	glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05)	967:1032	Furthermore, PPe-H could cause an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).					
30267825	1	18	contain	have	158:161	arg2	activities					182:191	various biological activities	163:191	various biological activities	163:191	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	1	18	contain	have	158:161	arg1	PPe					153:155	PPe	153:155	PPe	153:155	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	1	18	contain	have	158:161	arg1	polysaccharides					136:150	Pumpkin polysaccharides	128:150	Pumpkin polysaccharides (PPe)	128:156	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	3	19	theme	low-dose	491:498	arg1	streptozotocin					500:513	low-dose streptozotocin	491:513	low-dose streptozotocin	491:513	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	6	20	theme	GLP-1	986:990	arg1	secretion					992:1000	glucose stimulated GLP-1 secretion	967:1000	glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05)	967:1032	Furthermore, PPe-H could cause an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).					
30267825	3	21	theme	rat	422:424	arg1	model					426:430	A rat model	420:430	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin	420:513	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	8	22	theme	endogenous	1307:1316	arg1	GLP-1					1318:1322	endogenous GLP-1	1307:1322	endogenous GLP-1	1307:1322	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	4	23	theme	remarkable	689:698	arg1	ability					700:706	the remarkable ability	685:706	the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01)	685:834	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	2	24	theme	antidiabetic	362:373	arg1	activities					375:384	the antioxidant and antidiabetic activities	342:384	the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H)	342:417	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	0	25	theme	controlled	19:28	arg1	hydrolysates					35:46	the controlled acid hydrolysates	15:46	the controlled acid hydrolysates from pumpkin polysaccharides	15:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	6	26	theme	glucose	967:973	arg1	secretion					992:1000	glucose stimulated GLP-1 secretion	967:1000	glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05)	967:1032	Furthermore, PPe-H could cause an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).					
30267825	3	27	theme	type	435:438	arg1	T2DM					461:464	T2DM	461:464	T2DM	461:464	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	3	27	theme	type	435:438	arg1	mellitus					451:458	type 2 diabetes mellitus	435:458	type 2 diabetes mellitus (T2DM)	435:465	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	2	28	theme	composite	303:311	arg1	design					313:318	central composite design	295:318	central composite design (CCD)	295:324	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	2	28	theme	composite	303:311	arg1	CCD					321:323	CCD	321:323	CCD	321:323	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	0	29	theme	hydrolysates	35:46	arg1	Preparation					0:10	Preparation	0:10	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides	0:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	0	29	theme	hydrolysates	35:46	arg1	evaluation					116:125	their antioxidant and antidiabetic evaluation	81:125	their antioxidant and antidiabetic evaluation	81:125	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	4	30	theme	glucose	618:624	arg1	level					626:630	fasting blood glucose level	604:630	fasting blood glucose level	604:630	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	2	31	theme	hydrolysates	398:409	arg1	activities					375:384	the antioxidant and antidiabetic activities	342:384	the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H)	342:417	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	0	32	theme	acid	30:33	arg1	hydrolysates					35:46	the controlled acid hydrolysates	15:46	the controlled acid hydrolysates from pumpkin polysaccharides	15:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	2	33	theme	acid	393:396	arg1	hydrolysates					398:409	the acid hydrolysates	389:409	the acid hydrolysates (PPe-H)	389:417	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	2	33	theme	acid	393:396	arg1	PPe-H					412:416	PPe-H	412:416	PPe-H	412:416	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	0	34	theme	pumpkin	53:59	arg1	polysaccharides					61:75	pumpkin polysaccharides	53:75	pumpkin polysaccharides	53:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	2	35	with	PPe	255:257	arg1	ability					278:284	OH scavenging ability	264:284	OH scavenging ability	264:284	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	0	36	from	polysaccharides	61:75	arg1	hydrolysates					35:46	the controlled acid hydrolysates	15:46	the controlled acid hydrolysates from pumpkin polysaccharides	15:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	0	36	from	polysaccharides	61:75	arg1	Preparation					0:10	Preparation	0:10	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides	0:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	0	36	from	polysaccharides	61:75	arg1	evaluation					116:125	their antioxidant and antidiabetic evaluation	81:125	their antioxidant and antidiabetic evaluation	81:125	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	2	37	theme	hydrolysis	233:242	arg1	process					244:250	the acid hydrolysis process	224:250	the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD)	224:324	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	1	38	theme	Pumpkin	128:134	arg1	PPe					153:155	PPe	153:155	PPe	153:155	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	1	38	theme	Pumpkin	128:134	arg1	polysaccharides					136:150	Pumpkin polysaccharides	128:150	Pumpkin polysaccharides (PPe)	128:156	Pumpkin polysaccharides (PPe) have various biological activities.					
30267825	8	39	theme	diabetes	1392:1399	arg1	process					1381:1387	the process	1377:1387	the process of diabetes	1377:1399	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	4	40	theme	GSH	811:813	arg1	enzymes					753:759	the antioxidant enzymes	737:759	the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01)	737:792	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	40	theme	GSH	811:813	arg1	GR					770:771	GR	770:771	GR	770:771	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	40	theme	GSH	811:813	arg1	level					802:806	the level	798:806	the level of GSH (p < 0.05, p < 0.01)	798:834	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	40	theme	GSH	811:813	arg1	CAT					762:764	CAT	762:764	CAT	762:764	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	2	41	theme	acid	228:231	arg1	process					244:250	the acid hydrolysis process	224:250	the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD)	224:324	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	4	42	theme	enzymes	753:759	arg1	activities					723:732	the activities	719:732	the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01)	719:834	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	0	43	from	evaluation	116:125	arg1	polysaccharides					61:75	pumpkin polysaccharides	53:75	pumpkin polysaccharides	53:75	Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation.					
30267825	2	44	theme	scavenging	267:276	arg1	ability					278:284	OH scavenging ability	264:284	OH scavenging ability	264:284	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	4	45	theme	weight	645:650	arg1	loss					652:655	the weight loss	641:655	the weight loss in T2DM rats	641:668	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	3	46	theme	diabetes	442:449	arg1	T2DM					461:464	T2DM	461:464	T2DM	461:464	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	3	46	theme	diabetes	442:449	arg1	mellitus					451:458	type 2 diabetes mellitus	435:458	type 2 diabetes mellitus (T2DM)	435:465	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	2	47	theme	OH	264:265	arg1	ability					278:284	OH scavenging ability	264:284	OH scavenging ability	264:284	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	3	48	theme	mellitus	451:458	arg1	model					426:430	A rat model	420:430	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin	420:513	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	8	49	theme	potential	1216:1224	arg1	related					1267:1273	related	1267:1273	related	1267:1273	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	8	49	theme	potential	1216:1224	arg1	mechanism					1239:1247	The potential antidiabetic mechanism	1212:1247	The potential antidiabetic mechanism of PPe-H	1212:1256	The potential antidiabetic mechanism of PPe-H might be related to stimulating the secretion of endogenous GLP-1, decreasing oxidative damages, and then slowing down the process of diabetes.					
30267825	4	50	theme	antioxidant	741:751	arg1	enzymes					753:759	the antioxidant enzymes	737:759	the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01)	737:792	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	50	theme	antioxidant	741:751	arg1	GR					770:771	GR	770:771	GR	770:771	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	50	theme	antioxidant	741:751	arg1	CAT					762:764	CAT	762:764	CAT	762:764	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	51	theme	blood	612:616	arg1	level					626:630	fasting blood glucose level	604:630	fasting blood glucose level	604:630	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	52	theme	fasting	604:610	arg1	level					626:630	fasting blood glucose level	604:630	fasting blood glucose level	604:630	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	3	53	theme	high-fat	473:480	arg1	diet					482:485	high-fat diet	473:485	high-fat diet	473:485	A rat model of type 2 diabetes mellitus (T2DM) using high-fat diet and low-dose streptozotocin were established to assess the bioactivities.					
30267825	4	54	dep	enzymes	753:759	arg1	p < 0.01					784:791	p < 0.01	784:791	p < 0.01	784:791	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	54	dep	enzymes	753:759	arg1	enzymes					753:759	the antioxidant enzymes	737:759	the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01)	737:792	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	54	dep	enzymes	753:759	arg1	p < 0.01					774:781	p < 0.01	774:781	p < 0.01	774:781	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	54	dep	enzymes	753:759	arg1	GR					770:771	GR	770:771	GR	770:771	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	4	54	dep	enzymes	753:759	arg1	CAT					762:764	CAT	762:764	CAT	762:764	Both PPe and PPe-H could distinctly reduce fasting blood glucose level, prevent the weight loss in T2DM rats, and exhibited the remarkable ability to enhance the activities of the antioxidant enzymes (CAT and GR, p < 0.01, p < 0.01) and the level of GSH (p < 0.05, p < 0.01).					
30267825	5	55	theme	MDA	894:896	arg1	level					885:889	the level	881:889	the level of MDA (p < 0.05)	881:907	Besides, PPe-H could significantly decrease the level of MDA (p < 0.05).					
30267825	2	56	theme	PPe	255:257	arg1	process					244:250	the acid hydrolysis process	224:250	the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD)	224:324	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30267825	6	57	from	0 min	1007:1011	arg1	secretion					992:1000	glucose stimulated GLP-1 secretion	967:1000	glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05)	967:1032	Furthermore, PPe-H could cause an evident improvement to glucose stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).					
30267825	2	58	theme	antioxidant	346:356	arg1	activities					375:384	the antioxidant and antidiabetic activities	342:384	the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H)	342:417	This research was to optimize the acid hydrolysis process of PPe with OH scavenging ability based on central composite design (CCD), and to explore the antioxidant and antidiabetic activities of the acid hydrolysates (PPe-H).					
30170363	0	0	theme	Toosendan	91:99	arg1	polysaccharides					101:115	Fructus Meliae Toosendan polysaccharides	76:115	Fructus Meliae Toosendan polysaccharides	76:115	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	4	1	theme	response	864:871	arg1	method					881:886	the response surface method	860:886	the response surface method	860:886	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	10	2	from	Toosendan	1646:1654	arg1	polysaccharides					1610:1624	polysaccharides	1610:1624	polysaccharides from Fructus Meliae Toosendan	1610:1654	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	2	from	Toosendan	1646:1654	arg1	food					1734:1737	a functional food	1721:1737	a functional food	1721:1737	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	2	from	Toosendan	1646:1654	arg1	agent					1697:1701	a potential antioxidant agent	1673:1701	a potential antioxidant agent in medicine	1673:1713	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	1	3	theme	active	176:181	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides	147:161	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	1	3	theme	active	176:181	arg1	component					183:191	the main active component	167:191	the main active component of the Mongolian medicine Fructus Meliae Toosendan	167:242	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	4	4	theme	composite	773:781	arg1	tests					783:787	central composite tests	765:787	central composite tests on the four factors	765:807	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	9	5	theme	antioxidant	1582:1592	arg1	activity					1594:1601	considerable antioxidant activity	1569:1601	considerable antioxidant activity	1569:1601	The data show that FMTP has considerable antioxidant activity.					
30170363	8	6	theme	power	1528:1532	arg1	assay					1534:1538	power assay	1528:1538	power assay	1528:1538	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	5	7	theme	extraction	1051:1060	arg1	time					1062:1065	extraction time	1051:1065	extraction time of 20 min	1051:1075	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	4	8	from	tests	783:787	arg1	factors					801:807	the four factors	792:807	the four factors	792:807	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	10	9	theme	functional	1723:1732	arg1	polysaccharides					1610:1624	polysaccharides	1610:1624	polysaccharides from Fructus Meliae Toosendan	1610:1654	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	9	theme	functional	1723:1732	arg1	food					1734:1737	a functional food	1721:1737	a functional food	1721:1737	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	1	10	theme	antioxidant	280:290	arg1	effects					292:298	antioxidant effects	280:298	antioxidant effects	280:298	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	10	11	from	agent	1697:1701	arg1	medicine					1706:1713	medicine	1706:1713	medicine	1706:1713	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	6	12	theme	theoretical	1182:1192	arg1	predictions					1194:1204	the theoretical predictions	1178:1204	the theoretical predictions	1178:1204	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	6	13	theme	optimum	1142:1148	arg1	conditions					1150:1159	the optimum conditions	1138:1159	the optimum conditions	1138:1159	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	4	14	theme	central	765:771	arg1	tests					783:787	central composite tests	765:787	central composite tests on the four factors	765:807	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	5	15	theme	FMTP	956:959	arg1	extraction					971:980	FMTP microwave extraction	956:980	FMTP microwave extraction	956:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	0	16	theme	polysaccharides	101:115	arg1	methodology					61:71	the response surface methodology	40:71	the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity	40:144	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	4	17	theme	surface	873:879	arg1	method					881:886	the response surface method	860:886	the response surface method	860:886	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	2	18	from	extraction	369:378	arg1	FMTP					430:433	FMTP	430:433	FMTP	430:433	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	2	18	from	extraction	369:378	arg1	Toosendan					419:427	Toosendan	419:427	Toosendan	419:427	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	2	19	theme	polysaccharides	383:397	arg1	extraction					369:378	the microwave extraction	355:378	the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP)	355:434	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	6	20	from	conditions	1150:1159	arg1	rate					1119:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	6	20	from	conditions	1150:1159	arg1	%					1133:1133	15.75%	1128:1133	15.75%	1128:1133	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	6	21	theme	FMTP	1103:1106	arg1	rate					1119:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	6	21	theme	FMTP	1103:1106	arg1	%					1133:1133	15.75%	1128:1133	15.75%	1128:1133	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	0	22	theme	antioxidant	125:135	arg1	activity					137:144	its antioxidant activity	121:144	its antioxidant activity	121:144	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	3	23	theme	main	517:520	arg1	power					565:569	microwave power	555:569	microwave power	555:569	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	23	theme	main	517:520	arg1	time					583:586	extraction time	572:586	extraction time	572:586	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	23	theme	main	517:520	arg1	number					593:598	number	593:598	number of extractions	593:613	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	23	theme	main	517:520	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	23	theme	main	517:520	arg1	ratio					548:552	the solid-liquid ratio	531:552	the solid-liquid ratio	531:552	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	4	24	theme	optimization	832:843	arg1	analysis					845:852	an optimization analysis	829:852	an optimization analysis using the response surface method	829:886	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	7	25	theme	molecular	1320:1328	arg1	mass					1330:1333	a weight-average molecular mass	1303:1333	a weight-average molecular mass of 1288 Da	1303:1344	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	8	26	theme	antioxidant	1351:1361	arg1	activities					1363:1372	The antioxidant activities	1347:1372	The antioxidant activities of FMTP	1347:1380	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	3	27	theme	microwave	555:563	arg1	power					565:569	microwave power	555:569	microwave power	555:569	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	27	theme	microwave	555:563	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	8	28	theme	nitrite	1476:1482	arg1	scavenging					1497:1506	nitrite free radical scavenging	1476:1506	nitrite free radical scavenging	1476:1506	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	7	29	theme	1288 Da	1338:1344	arg1	mass					1330:1333	a weight-average molecular mass	1303:1333	a weight-average molecular mass of 1288 Da	1303:1344	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	0	30	theme	extraction	10:19	arg1	optimization					21:32	Microwave extraction optimization	0:32	Microwave extraction optimization	0:32	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	8	31	theme	anion	1427:1431	arg1	assays					1508:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	9	32	contain	has	1565:1567	arg1	FMTP					1560:1563	FMTP	1560:1563	FMTP	1560:1563	The data show that FMTP has considerable antioxidant activity.					
30170363	9	32	contain	has	1565:1567	arg2	activity					1594:1601	considerable antioxidant activity	1569:1601	considerable antioxidant activity	1569:1601	The data show that FMTP has considerable antioxidant activity.					
30170363	5	33	theme	optimum	933:939	arg1	conditions					941:950	the optimum conditions	929:950	the optimum conditions for FMTP microwave extraction	929:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	7	34	theme	high-purity	1263:1273	arg1	polysaccharide					1280:1293	high-purity FMTP polysaccharide	1263:1293	high-purity FMTP polysaccharide	1263:1293	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	1	35	theme	Mongolian	200:208	arg1	medicine					210:217	the Mongolian medicine Fructus Meliae Toosendan	196:242	the Mongolian medicine Fructus Meliae Toosendan	196:242	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	0	36	theme	Microwave	0:8	arg1	optimization					21:32	Microwave extraction optimization	0:32	Microwave extraction optimization	0:32	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	9	37	theme	considerable	1569:1580	arg1	activity					1594:1601	considerable antioxidant activity	1569:1601	considerable antioxidant activity	1569:1601	The data show that FMTP has considerable antioxidant activity.					
30170363	8	38	theme	free	1484:1487	arg1	scavenging					1497:1506	nitrite free radical scavenging	1476:1506	nitrite free radical scavenging	1476:1506	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	1	39	theme	medicine	210:217	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides	147:161	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	1	39	theme	medicine	210:217	arg1	component					183:191	the main active component	167:191	the main active component of the Mongolian medicine Fructus Meliae Toosendan	167:242	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	8	40	theme	scavenging	1497:1506	arg1	assays					1508:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	3	41	theme	extraction	572:581	arg1	time					583:586	extraction time	572:586	extraction time	572:586	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	41	theme	extraction	572:581	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	2	42	theme	response	446:453	arg1	method					465:470	the response interface method	442:470	the response interface method	442:470	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	2	43	theme	interface	455:463	arg1	method					465:470	the response interface method	442:470	the response interface method	442:470	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	6	44	with	consistent	1162:1171	arg1	predictions					1194:1204	the theoretical predictions	1178:1204	the theoretical predictions	1178:1204	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	0	45	theme	activity	137:144	arg1	methodology					61:71	the response surface methodology	40:71	the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity	40:144	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	4	46	theme	response	749:756	arg1	index					758:762	a response index	747:762	a response index	747:762	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	4	46	theme	response	749:756	arg1	content					736:742	the FMTP content	727:742	the FMTP content	727:742	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	7	47	theme	weight-average	1305:1318	arg1	mass					1330:1333	a weight-average molecular mass	1303:1333	a weight-average molecular mass of 1288 Da	1303:1344	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	0	48	theme	response	44:51	arg1	methodology					61:71	the response surface methodology	40:71	the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity	40:144	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	5	49	theme	microwave	961:969	arg1	extraction					971:980	FMTP microwave extraction	956:980	FMTP microwave extraction	956:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	3	50	theme	microwave	668:676	arg1	process					689:695	the microwave extraction process	664:695	the microwave extraction process	664:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	51	theme	extractions	603:613	arg1	power					565:569	microwave power	555:569	microwave power	555:569	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	51	theme	extractions	603:613	arg1	time					583:586	extraction time	572:586	extraction time	572:586	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	51	theme	extractions	603:613	arg1	number					593:598	number	593:598	number of extractions	593:613	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	51	theme	extractions	603:613	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	51	theme	extractions	603:613	arg1	ratio					548:552	the solid-liquid ratio	531:552	the solid-liquid ratio	531:552	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	10	52	theme	antioxidant	1685:1695	arg1	polysaccharides					1610:1624	polysaccharides	1610:1624	polysaccharides from Fructus Meliae Toosendan	1610:1654	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	52	theme	antioxidant	1685:1695	arg1	agent					1697:1701	a potential antioxidant agent	1673:1701	a potential antioxidant agent in medicine	1673:1713	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	1	53	dep	medicine	210:217	arg1	Toosendan					234:242	Toosendan	234:242	Toosendan	234:242	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	2	54	from	Toosendan	419:427	arg1	polysaccharides					383:397	polysaccharides	383:397	polysaccharides from Fructus Meliae Toosendan (FMTP)	383:434	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	2	54	from	Toosendan	419:427	arg1	extraction					369:378	the microwave extraction	355:378	the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP)	355:434	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	10	55	theme	potential	1675:1683	arg1	polysaccharides					1610:1624	polysaccharides	1610:1624	polysaccharides from Fructus Meliae Toosendan	1610:1654	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	55	theme	potential	1675:1683	arg1	agent					1697:1701	a potential antioxidant agent	1673:1701	a potential antioxidant agent in medicine	1673:1713	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	8	56	theme	FMTP	1377:1380	arg1	activities					1363:1372	The antioxidant activities	1347:1372	The antioxidant activities of FMTP	1347:1380	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	8	57	theme	superoxide	1416:1425	arg1	anion					1427:1431	hydroxyl superoxide anion	1407:1431	hydroxyl superoxide anion	1407:1431	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	2	58	theme	extraction	369:378	arg1	optimization					339:350	the optimization	335:350	the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method	335:470	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	0	59	theme	surface	53:59	arg1	methodology					61:71	the response surface methodology	40:71	the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity	40:144	Microwave extraction optimization using the response surface methodology of Fructus Meliae Toosendan polysaccharides and its antioxidant activity.					
30170363	5	60	theme	700 W	1044:1048	arg1	ratio					1002:1006	a solvent-material ratio	983:1006	a solvent-material ratio of 30.00 mL/mg	983:1021	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	60	theme	700 W	1044:1048	arg1	time					1062:1065	extraction time	1051:1065	extraction time of 20 min	1051:1075	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	60	theme	700 W	1044:1048	arg1	power					1035:1039	extraction power	1024:1039	extraction power of 700 W	1024:1048	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	60	theme	700 W	1044:1048	arg1	conditions					941:950	the optimum conditions	929:950	the optimum conditions for FMTP microwave extraction	929:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	60	theme	700 W	1044:1048	arg1	extractions					1086:1096	two extractions	1082:1096	two extractions	1082:1096	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	8	61	theme	hydroxyl	1407:1414	arg1	anion					1427:1431	hydroxyl superoxide anion	1407:1431	hydroxyl superoxide anion	1407:1431	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	7	62	theme	FMTP	1275:1278	arg1	polysaccharide					1280:1293	high-purity FMTP polysaccharide	1263:1293	high-purity FMTP polysaccharide	1263:1293	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	2	63	theme	microwave	359:367	arg1	extraction					369:378	the microwave extraction	355:378	the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP)	355:434	Therefore, the optimization of the microwave extraction of polysaccharides from Fructus Meliae Toosendan (FMTP) using the response interface method was carried out.					
30170363	8	64	theme	2,2-diphenyl-1-picrylhydrazyl	1434:1462	arg1	assays					1508:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays	1403:1513	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	5	65	theme	extraction	1024:1033	arg1	power					1035:1039	extraction power	1024:1039	extraction power of 700 W	1024:1048	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	66	theme	solvent-material	985:1000	arg1	ratio					1002:1006	a solvent-material ratio	983:1006	a solvent-material ratio of 30.00 mL/mg	983:1021	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	3	67	theme	polysaccharide	630:643	arg1	rate					656:659	the polysaccharide extraction rate	626:659	the polysaccharide extraction rate	626:659	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	68	from	tests	503:507	arg1	power					565:569	microwave power	555:569	microwave power	555:569	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	68	from	tests	503:507	arg1	time					583:586	extraction time	572:586	extraction time	572:586	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	68	from	tests	503:507	arg1	number					593:598	number	593:598	number of extractions	593:613	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	68	from	tests	503:507	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	68	from	tests	503:507	arg1	ratio					548:552	the solid-liquid ratio	531:552	the solid-liquid ratio	531:552	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	6	69	theme	extraction	1108:1117	arg1	rate					1119:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate	1099:1122	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	6	69	theme	extraction	1108:1117	arg1	%					1133:1133	15.75%	1128:1133	15.75%	1128:1133	The FMTP extraction rate was 15.75% at the optimum conditions, consistent with the theoretical predictions.					
30170363	3	70	dep	factors	522:528	arg1	power					565:569	microwave power	555:569	microwave power	555:569	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	70	dep	factors	522:528	arg1	time					583:586	extraction time	572:586	extraction time	572:586	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	70	dep	factors	522:528	arg1	number					593:598	number	593:598	number of extractions	593:613	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	70	dep	factors	522:528	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	70	dep	factors	522:528	arg1	ratio					548:552	the solid-liquid ratio	531:552	the solid-liquid ratio	531:552	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	71	theme	extraction	678:687	arg1	process					689:695	the microwave extraction process	664:695	the microwave extraction process	664:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	72	theme	extraction	645:654	arg1	rate					656:659	the polysaccharide extraction rate	626:659	the polysaccharide extraction rate	626:659	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	5	73	theme	20 min	1070:1075	arg1	ratio					1002:1006	a solvent-material ratio	983:1006	a solvent-material ratio of 30.00 mL/mg	983:1021	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	73	theme	20 min	1070:1075	arg1	time					1062:1065	extraction time	1051:1065	extraction time of 20 min	1051:1075	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	73	theme	20 min	1070:1075	arg1	power					1035:1039	extraction power	1024:1039	extraction power of 700 W	1024:1048	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	73	theme	20 min	1070:1075	arg1	conditions					941:950	the optimum conditions	929:950	the optimum conditions for FMTP microwave extraction	929:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	73	theme	20 min	1070:1075	arg1	extractions					1086:1096	two extractions	1082:1096	two extractions	1082:1096	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	4	74	theme	FMTP	731:734	arg1	index					758:762	a response index	747:762	a response index	747:762	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	4	74	theme	FMTP	731:734	arg1	content					736:742	the FMTP content	727:742	the FMTP content	727:742	Then, using the FMTP content as a response index, central composite tests on the four factors were conducted, and an optimization analysis using the response surface method was completed.					
30170363	7	75	theme	crude	1211:1215	arg1	polysaccharide					1217:1230	The crude polysaccharide	1207:1230	The crude polysaccharide	1207:1230	The crude polysaccharide was further purified to obtain high-purity FMTP polysaccharide, having a weight-average molecular mass of 1288 Da.					
30170363	8	76	theme	radical	1489:1495	arg1	scavenging					1497:1506	nitrite free radical scavenging	1476:1506	nitrite free radical scavenging	1476:1506	The antioxidant activities of FMTP were evaluated using the hydroxyl superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitrite free radical scavenging assays and reducing power assay.					
30170363	3	77	theme	Single-factor	489:501	arg1	tests					503:507	Single-factor tests	489:507	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	489:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	78	theme	solid-liquid	535:546	arg1	factors					522:528	four main factors	512:528	four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process	512:695	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	3	78	theme	solid-liquid	535:546	arg1	ratio					548:552	the solid-liquid ratio	531:552	the solid-liquid ratio	531:552	Single-factor tests on four main factors (the solid-liquid ratio, microwave power, extraction time, and number of extractions) affecting the polysaccharide extraction rate in the microwave extraction process were carried out.					
30170363	1	79	theme	effective	255:263	arg1	extraction					265:274	their effective extraction	249:274	their effective extraction	249:274	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	5	80	theme	30.00 mL/mg	1011:1021	arg1	ratio					1002:1006	a solvent-material ratio	983:1006	a solvent-material ratio of 30.00 mL/mg	983:1021	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	80	theme	30.00 mL/mg	1011:1021	arg1	time					1062:1065	extraction time	1051:1065	extraction time of 20 min	1051:1075	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	80	theme	30.00 mL/mg	1011:1021	arg1	power					1035:1039	extraction power	1024:1039	extraction power of 700 W	1024:1048	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	80	theme	30.00 mL/mg	1011:1021	arg1	conditions					941:950	the optimum conditions	929:950	the optimum conditions for FMTP microwave extraction	929:980	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	5	80	theme	30.00 mL/mg	1011:1021	arg1	extractions					1086:1096	two extractions	1082:1096	two extractions	1082:1096	Consequently, we obtained the optimum conditions for FMTP microwave extraction: a solvent-material ratio of 30.00 mL/mg, extraction power of 700 W, extraction time of 20 min, and two extractions.					
30170363	1	81	theme	main	171:174	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides	147:161	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	1	81	theme	main	171:174	arg1	component					183:191	the main active component	167:191	the main active component of the Mongolian medicine Fructus Meliae Toosendan	167:242	Polysaccharides are the main active component of the Mongolian medicine Fructus Meliae Toosendan, but their effective extraction and antioxidant effects have not been reported.					
30170363	10	82	used	used	1665:1668	arg2	food					1734:1737	a functional food	1721:1737	a functional food	1721:1737	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	82	used	used	1665:1668	arg2	agent					1697:1701	a potential antioxidant agent	1673:1701	a potential antioxidant agent in medicine	1673:1713	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
30170363	10	82	used	used	1665:1668	arg2	polysaccharides					1610:1624	polysaccharides	1610:1624	polysaccharides from Fructus Meliae Toosendan	1610:1654	Thus, polysaccharides from Fructus Meliae Toosendan could be used as a potential antioxidant agent in medicine or as a functional food.					
29486260	0	0	theme	polysaccharide-protein	79:100	arg1	conjugate					102:110	the polysaccharide-protein conjugate	75:110	the polysaccharide-protein conjugate	75:110	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	4	1	from	profiles	599:606	arg1	seeds					636:640	ungerminated seeds	623:640	ungerminated seeds	623:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	2	2	theme	seeds	338:342	arg1	composition					323:333	the composition	319:333	the composition of seeds	319:342	During germination, the composition of seeds undergoes distinct changes that cause variations in bioactivities.					
29486260	6	3	theme	2,2-diphenyl-1-picrylhydrazyl	954:982	arg1	activities					1068:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	3	theme	2,2-diphenyl-1-picrylhydrazyl	954:982	arg1	highest					1113:1119	highest	1113:1119	highest	1113:1119	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	5	4	from	polysaccharide	743:756	arg1	fraction					764:771	F1 fraction	761:771	F1 fraction of ungerminated seeds	761:793	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	1	5	theme	Black	154:158	arg1	soybeans					160:167	Black soybeans	154:167	Black soybeans	154:167	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	1	5	theme	Black	154:158	arg1	foods					201:205	health foods	194:205	health foods	194:205	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	7	6	theme	phenolic	1160:1167	arg1	content					1183:1189	The phenolic and flavonoid content	1156:1189	content	1183:1189	The phenolic and flavonoid content significantly increased after the fifth day of germination, suggesting that these ingredients also contributed to the antioxidant activities.					
29486260	0	7	from	Effect	0:5	arg1	profiling					37:45	composition profiling	25:45	composition profiling	25:45	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	0	7	from	Effect	0:5	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	8	8	theme	F1	1407:1408	arg1	fraction					1410:1417	the F1 fraction	1403:1417	the F1 fraction	1403:1417	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	6	9	theme	germination	1143:1153	arg1	day					1136:1138	the seventh day	1124:1138	the seventh day of germination	1124:1153	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	0	10	theme	conjugate	102:110	arg1	profiling					37:45	composition profiling	25:45	composition profiling	25:45	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	0	10	theme	conjugate	102:110	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	8	11	theme	foods	1537:1541	arg1	development					1511:1521	the development	1507:1521	the development of functional foods	1507:1541	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	5	12	theme	various	915:921	arg1	levels					923:928	various levels	915:928	various levels during germination	915:947	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	7	13	theme	germination	1238:1248	arg1	day					1231:1233	the fifth day	1221:1233	the fifth day of germination	1221:1248	The phenolic and flavonoid content significantly increased after the fifth day of germination, suggesting that these ingredients also contributed to the antioxidant activities.					
29486260	0	14	theme	soybean	121:127	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	8	15	theme	functional	1526:1535	arg1	foods					1537:1541	functional foods	1526:1541	functional foods	1526:1541	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	5	16	theme	seeds	789:793	arg1	fraction					764:771	F1 fraction	761:771	F1 fraction of ungerminated seeds	761:793	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	4	17	theme	BSPS	615:618	arg1	profiles					599:606	The chromatographic profiles	579:606	The chromatographic profiles of the BSPS in ungerminated seeds	579:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	8	18	theme	long-term	1340:1348	arg1	germination					1350:1360	long-term germination	1340:1360	long-term germination	1340:1360	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	6	19	theme	3-ethylbenzthiazoline-6-sulphonic	1010:1042	arg1	2,2'-azino-bis					995:1008	2,2'-azino-bis	995:1008	2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)	995:1055	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	19	theme	3-ethylbenzthiazoline-6-sulphonic	1010:1042	arg1	acid					1044:1047	3-ethylbenzthiazoline-6-sulphonic acid	1010:1047	3-ethylbenzthiazoline-6-sulphonic acid	1010:1047	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	0	20	theme	black	115:119	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	0	21	from	profiling	37:45	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	3	22	theme	two-day	519:525	arg1	intervals					527:535	two-day intervals	519:535	two-day intervals during the first week of seedling growth	519:576	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	6	23	theme	scavenging	1057:1066	arg1	activities					1068:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	23	theme	scavenging	1057:1066	arg1	highest					1113:1119	highest	1113:1119	highest	1113:1119	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	7	24	theme	antioxidant	1309:1319	arg1	activities					1321:1330	the antioxidant activities	1305:1330	the antioxidant activities	1305:1330	The phenolic and flavonoid content significantly increased after the fifth day of germination, suggesting that these ingredients also contributed to the antioxidant activities.					
29486260	8	25	from	conjugate	1390:1398	arg1	fraction					1410:1417	the F1 fraction	1403:1417	the F1 fraction	1403:1417	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	5	26	theme	F1	761:762	arg1	fraction					764:771	F1 fraction	761:771	F1 fraction of ungerminated seeds	761:793	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	8	27	theme	natural	1483:1489	arg1	conjugate					1390:1398	the polysaccharide-protein conjugate	1363:1398	the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities	1363:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	8	27	theme	natural	1483:1489	arg1	antioxidant					1491:1501	a potential natural antioxidant	1471:1501	a potential natural antioxidant for the development of functional foods	1471:1541	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	4	28	from	seeds	636:640	arg1	profiles					599:606	The chromatographic profiles	579:606	The chromatographic profiles of the BSPS in ungerminated seeds	579:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	8	29	theme	antioxidant	1433:1443	arg1	activities					1445:1454	enhanced antioxidant activities	1424:1454	enhanced antioxidant activities	1424:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	5	30	theme	galacturonic	894:905	arg1	arabinose					859:867	arabinose	859:867	arabinose	859:867	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	5	30	theme	galacturonic	894:905	arg1	acid					907:910	galacturonic acid	894:910	galacturonic acid	894:910	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	3	31	theme	soybean	450:456	arg1	BSPS					474:477	BSPS	474:477	BSPS	474:477	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	3	31	theme	soybean	450:456	arg1	polysaccharide					458:471	the water-soluble black soybean polysaccharide	426:471	the water-soluble black soybean polysaccharide (BSPS)	426:478	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	0	32	theme	germination	10:20	arg1	Effect					0:5	Effect	0:5	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.	0:150	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	0	33	theme	[Glycinemax	129:139	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	3	34	theme	first	548:552	arg1	week					554:557	the first week	544:557	the first week of seedling growth	544:576	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	1	35	theme	health	194:199	arg1	soybeans					160:167	Black soybeans	154:167	Black soybeans	154:167	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	1	35	theme	health	194:199	arg1	foods					201:205	health foods	194:205	health foods	194:205	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	8	36	with	conjugate	1390:1398	arg1	activities					1445:1454	enhanced antioxidant activities	1424:1454	enhanced antioxidant activities	1424:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	7	37	theme	fifth	1225:1229	arg1	day					1231:1233	the fifth day	1221:1233	the fifth day of germination	1221:1248	The phenolic and flavonoid content significantly increased after the fifth day of germination, suggesting that these ingredients also contributed to the antioxidant activities.					
29486260	4	38	theme	ungerminated	623:634	arg1	seeds					636:640	ungerminated seeds	623:640	ungerminated seeds	623:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	4	39	from	BSPS	615:618	arg1	seeds					636:640	ungerminated seeds	623:640	ungerminated seeds	623:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	3	40	theme	water-soluble	430:442	arg1	BSPS					474:477	BSPS	474:477	BSPS	474:477	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	3	40	theme	water-soluble	430:442	arg1	polysaccharide					458:471	the water-soluble black soybean polysaccharide	426:471	the water-soluble black soybean polysaccharide (BSPS)	426:478	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	0	41	theme	composition	25:35	arg1	profiling					37:45	composition profiling	25:45	composition profiling	25:45	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	1	42	theme	edible	283:288	arg1	sprouts					290:296	edible sprouts	283:296	edible sprouts	283:296	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	1	42	theme	edible	283:288	arg1	they					253:256	they	253:256	they	253:256	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	0	43	theme	L.	142:143	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	3	44	theme	black	444:448	arg1	BSPS					474:477	BSPS	474:477	BSPS	474:477	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	3	44	theme	black	444:448	arg1	polysaccharide					458:471	the water-soluble black soybean polysaccharide	426:471	the water-soluble black soybean polysaccharide (BSPS)	426:478	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	0	45	theme	antioxidant	51:61	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	6	46	theme	2,2'-azino-bis	995:1008	arg1	activities					1068:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	46	theme	2,2'-azino-bis	995:1008	arg1	highest					1113:1119	highest	1113:1119	highest	1113:1119	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	0	47	from	activity	63:70	arg1	Merr					146:149	black soybean [Glycinemax (L.) Merr	115:149	black soybean [Glycinemax (L.) Merr	115:149	Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.]					
29486260	3	48	theme	seedling	562:569	arg1	growth					571:576	seedling growth	562:576	seedling growth	562:576	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	6	49	theme	F1	1105:1106	arg1	power					1096:1100	the reducing power	1083:1100	the reducing power of F1	1083:1106	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	49	theme	F1	1105:1106	arg1	activities					1068:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities	950:1077	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	49	theme	F1	1105:1106	arg1	highest					1113:1119	highest	1113:1119	highest	1113:1119	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	8	50	theme	potential	1473:1481	arg1	conjugate					1390:1398	the polysaccharide-protein conjugate	1363:1398	the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities	1363:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	8	50	theme	potential	1473:1481	arg1	antioxidant					1491:1501	a potential natural antioxidant	1471:1501	a potential natural antioxidant for the development of functional foods	1471:1541	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	3	51	theme	growth	571:576	arg1	week					554:557	the first week	544:557	the first week of seedling growth	544:576	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	2	52	from	variations	382:391	arg1	bioactivities					396:408	bioactivities	396:408	bioactivities	396:408	During germination, the composition of seeds undergoes distinct changes that cause variations in bioactivities.					
29486260	3	53	attach	isolated	484:491	arg2	polysaccharide					458:471	the water-soluble black soybean polysaccharide	426:471	the water-soluble black soybean polysaccharide (BSPS)	426:478	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	3	53	attach	isolated	484:491	arg1	sprouts					498:504	sprouts	498:504	sprouts harvested at two-day intervals during the first week of seedling growth	498:576	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	3	53	attach	isolated	484:491	arg2	BSPS					474:477	BSPS	474:477	BSPS	474:477	In this study, the water-soluble black soybean polysaccharide (BSPS) was isolated from sprouts harvested at two-day intervals during the first week of seedling growth.					
29486260	4	54	dep	F2	694:695	arg1	<1kDa					698:702	<1kDa	698:702	<1kDa	698:702	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	8	55	theme	polysaccharide-protein	1367:1388	arg1	conjugate					1390:1398	the polysaccharide-protein conjugate	1363:1398	the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities	1363:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	8	55	theme	polysaccharide-protein	1367:1388	arg1	antioxidant					1491:1501	a potential natural antioxidant	1471:1501	a potential natural antioxidant for the development of functional foods	1471:1541	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	6	56	theme	seventh	1128:1134	arg1	day					1136:1138	the seventh day	1124:1138	the seventh day of germination	1124:1153	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	7	57	theme	flavonoid	1173:1181	arg1	content					1183:1189	The phenolic and flavonoid content	1156:1189	content	1183:1189	The phenolic and flavonoid content significantly increased after the fifth day of germination, suggesting that these ingredients also contributed to the antioxidant activities.					
29486260	6	58	theme	reducing	1087:1094	arg1	power					1096:1100	the reducing power	1083:1100	the reducing power of F1	1083:1106	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	6	58	theme	reducing	1087:1094	arg1	highest					1113:1119	highest	1113:1119	highest	1113:1119	The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activities and the reducing power of F1 were highest on the seventh day of germination.					
29486260	4	59	theme	chromatographic	583:597	arg1	profiles					599:606	The chromatographic profiles	579:606	The chromatographic profiles of the BSPS in ungerminated seeds	579:640	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	1	60	used	used	211:214	arg2	soybeans					160:167	Black soybeans	154:167	Black soybeans	154:167	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	1	60	used	used	211:214	arg2	foods					201:205	health foods	194:205	health foods	194:205	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	4	61	dep	fraction	649:656	arg1	F1					661:662	F1	661:662	F1	661:662	The chromatographic profiles of the BSPS in ungerminated seeds showed fraction 1 (F1, about 64kDa) and fraction 2 (F2, <1kDa) that degraded during germination.					
29486260	5	62	theme	ungerminated	776:787	arg1	seeds					789:793	ungerminated seeds	776:793	ungerminated seeds	776:793	The polysaccharide in F1 fraction of ungerminated seeds was covalently associated with the protein and mainly contained arabinose, galactose, glucose, and galacturonic acid at various levels during germination.					
29486260	2	63	theme	distinct	354:361	arg1	changes					363:369	distinct changes	354:369	distinct changes that cause variations in bioactivities	354:408	During germination, the composition of seeds undergoes distinct changes that cause variations in bioactivities.					
29486260	1	64	theme	traditional	219:229	arg1	medicine					239:246	traditional Chinese medicine	219:246	traditional Chinese medicine	219:246	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
29486260	8	65	theme	enhanced	1424:1431	arg1	activities					1445:1454	enhanced antioxidant activities	1424:1454	enhanced antioxidant activities	1424:1454	During long-term germination, the polysaccharide-protein conjugate in the F1 fraction with enhanced antioxidant activities is regarded as a potential natural antioxidant for the development of functional foods.					
29486260	1	66	theme	Chinese	231:237	arg1	medicine					239:246	traditional Chinese medicine	219:246	traditional Chinese medicine	219:246	Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts.					
30171958	6	0	theme	Structural	566:575	arg1	analysis					577:584	Structural analysis	566:584	Structural analysis	566:584	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	9	1	theme	Activity	1026:1033	arg1	studies					1035:1041	Activity studies	1026:1041	Activity studies	1026:1041	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	9	2	theme	natural	1062:1068	arg1	polysaccharides					1070:1084	natural polysaccharides	1062:1084	natural polysaccharides	1062:1084	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	0	3	theme	jujuba	89:94	arg1	cv					96:97	Zizyphus jujuba cv	80:97	Zizyphus jujuba cv	80:97	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	2	4	theme	jujuba	226:231	arg1	cv					233:234	Zizyphus jujuba cv	217:234	Zizyphus jujuba cv	217:234	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	11	5	theme	biological	1486:1495	arg1	characteristics					1497:1511	their biological characteristics	1480:1511	their biological characteristics	1480:1511	The degree of substitution of polysaccharides seemed to be closely related to their biological characteristics.					
30171958	7	6	theme	galacturonic	754:765	arg1	composition					779:789	galacturonic acid (GalA) composition	754:789	galacturonic acid (GalA) composition	754:789	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	9	7	theme	sulfated	1162:1169	arg1	polysaccharides					1180:1194	sulfated modified polysaccharides	1162:1194	sulfated modified polysaccharides	1162:1194	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	9	8	theme	immunomodulatory	1129:1144	arg1	activity					1146:1153	immunomodulatory activity	1129:1153	immunomodulatory activity	1129:1153	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	6	9	theme	average	664:670	arg1	weight					682:687	the average molecular weight	660:687	the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa	660:720	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	9	theme	average	664:670	arg1	Mw					690:691	Mw	690:691	Mw	690:691	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	12	10	theme	jujuba	1633:1638	arg1	polysaccharides					1640:1654	jujuba polysaccharides	1633:1654	jujuba polysaccharides	1633:1654	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	7	11	theme	arabinose	848:856	arg1	Ara					859:861	Ara	859:861	Ara	859:861	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	11	theme	arabinose	848:856	arg1	Gal					839:841	Gal	839:841	Gal	839:841	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	11	theme	arabinose	848:856	arg1	arabinose					848:856	arabinose	848:856	arabinose (Ara)	848:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	11	theme	arabinose	848:856	arg1	galactose					828:836	galactose	828:836	galactose (Gal)	828:842	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	11	theme	arabinose	848:856	arg1	amount					818:823	a small amount	810:823	a small amount of galactose (Gal) and arabinose (Ara)	810:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	6	12	theme	357.39 kDa	711:720	arg1	weight					682:687	the average molecular weight	660:687	the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa	660:720	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	12	theme	357.39 kDa	711:720	arg1	Mw					690:691	Mw	690:691	Mw	690:691	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	10	13	theme	thromboplastin	1281:1294	arg1	APTT					1302:1305	APTT	1302:1305	APTT	1302:1305	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	13	theme	thromboplastin	1281:1294	arg1	time					1296:1299	partial thromboplastin time	1273:1299	partial thromboplastin time (APTT)	1273:1306	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	14	theme	sulfated	1224:1231	arg1	polysaccharides					1233:1247	the sulfated polysaccharides	1220:1247	the sulfated polysaccharides	1220:1247	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	15	theme	partial	1273:1279	arg1	APTT					1302:1305	APTT	1302:1305	APTT	1302:1305	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	15	theme	partial	1273:1279	arg1	time					1296:1299	partial thromboplastin time	1273:1299	partial thromboplastin time (APTT)	1273:1306	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	6	16	with	polysaccharides	639:653	arg1	weight					682:687	the average molecular weight	660:687	the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa	660:720	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	16	with	polysaccharides	639:653	arg1	Mw					690:691	Mw	690:691	Mw	690:691	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	17	theme	size	626:629	arg1	polysaccharides					639:653	size uniform polysaccharides	626:653	size uniform polysaccharides	626:653	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	4	18	theme	high-performance	331:346	arg1	chromatography					363:376	high-performance gel permeation chromatography	331:376	high-performance gel permeation chromatography (HPGPC)	331:384	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	18	theme	high-performance	331:346	arg1	HPGPC					379:383	HPGPC	379:383	HPGPC	379:383	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	6	19	theme	uniform	631:637	arg1	polysaccharides					639:653	size uniform polysaccharides	626:653	size uniform polysaccharides	626:653	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	12	20	theme	immunomodulatory	1584:1599	arg1	activities					1619:1628	the immunomodulatory and anticoagulant activities	1580:1628	the immunomodulatory and anticoagulant activities of jujuba polysaccharides	1580:1654	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	4	21	theme	magnetic	395:402	arg1	NMR					415:417	NMR	415:417	NMR	415:417	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	21	theme	magnetic	395:402	arg1	resonance					404:412	nuclear magnetic resonance	387:412	nuclear magnetic resonance (NMR)	387:418	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	5	22	theme	chlorosulfonic	526:539	arg1	CSA-Pyr					556:562	CSA-Pyr	556:562	CSA-Pyr	556:562	Further, eight sulfated derivatives are prepared by chlorosulfonic acid-pyridine (CSA-Pyr).					
30171958	5	22	theme	chlorosulfonic	526:539	arg1	acid-pyridine					541:553	chlorosulfonic acid-pyridine	526:553	chlorosulfonic acid-pyridine (CSA-Pyr)	526:563	Further, eight sulfated derivatives are prepared by chlorosulfonic acid-pyridine (CSA-Pyr).					
30171958	0	23	theme	Sulfated	0:7	arg1	modification					9:20	Sulfated modification	0:20	Sulfated modification	0:20	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	7	24	theme	small	812:816	arg1	Ara					859:861	Ara	859:861	Ara	859:861	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	24	theme	small	812:816	arg1	Gal					839:841	Gal	839:841	Gal	839:841	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	24	theme	small	812:816	arg1	arabinose					848:856	arabinose	848:856	arabinose (Ara)	848:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	24	theme	small	812:816	arg1	galactose					828:836	galactose	828:836	galactose (Gal)	828:842	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	24	theme	small	812:816	arg1	amount					818:823	a small amount	810:823	a small amount of galactose (Gal) and arabinose (Ara)	810:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	9	25	theme	modified	1171:1178	arg1	polysaccharides					1180:1194	sulfated modified polysaccharides	1162:1194	sulfated modified polysaccharides	1162:1194	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	7	26	contain	contains	801:808	arg2	amount					818:823	a small amount	810:823	a small amount of galactose (Gal) and arabinose (Ara)	810:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	26	contain	contains	801:808	arg2	Ara					859:861	Ara	859:861	Ara	859:861	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	26	contain	contains	801:808	arg2	arabinose					848:856	arabinose	848:856	arabinose (Ara)	848:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	26	contain	contains	801:808	arg1	JCS-1					737:741	JCS-1	737:741	JCS-1	737:741	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	26	contain	contains	801:808	arg2	galactose					828:836	galactose	828:836	galactose (Gal)	828:842	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	26	contain	contains	801:808	arg2	Gal					839:841	Gal	839:841	Gal	839:841	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	4	27	theme	liquid	301:306	arg1	chromatography					308:321	high performance liquid chromatography	284:321	high performance liquid chromatography (HPLC)	284:328	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	27	theme	liquid	301:306	arg1	HPLC					324:327	HPLC	324:327	HPLC	324:327	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	10	28	theme	clotting	1387:1394	arg1	PT					1383:1384	PT	1383:1384	PT	1383:1384	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	28	theme	clotting	1387:1394	arg1	time					1396:1399	prothrombin time (PT) clotting time	1365:1399	prothrombin time (PT) clotting time	1365:1399	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	0	29	theme	biological	26:35	arg1	activities					37:46	biological activities	26:46	biological activities	26:46	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	10	30	theme	prothrombin	1365:1375	arg1	PT					1383:1384	PT	1383:1384	PT	1383:1384	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	30	theme	prothrombin	1365:1375	arg1	time					1396:1399	prothrombin time (PT) clotting time	1365:1399	prothrombin time (PT) clotting time	1365:1399	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	4	31	theme	performance	289:299	arg1	chromatography					308:321	high performance liquid chromatography	284:321	high performance liquid chromatography (HPLC)	284:328	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	31	theme	performance	289:299	arg1	HPLC					324:327	HPLC	324:327	HPLC	324:327	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	9	32	theme	sulfated	1090:1097	arg1	polysaccharides					1108:1122	sulfated modified polysaccharides	1090:1122	sulfated modified polysaccharides	1090:1122	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	8	33	theme	molar	984:988	arg1	ratio					990:994	a molar ratio	982:994	a molar ratio of 19.87:2.07:1.77:1.65:1.16	982:1023	JCS-2 was composed of GalA, mannose (Man), rhamnose (Rha), Ara and Gal, at a molar ratio of 19.87:2.07:1.77:1.65:1.16.					
30171958	5	34	theme	sulfated	489:496	arg1	derivatives					498:508	eight sulfated derivatives	483:508	eight sulfated derivatives	483:508	Further, eight sulfated derivatives are prepared by chlorosulfonic acid-pyridine (CSA-Pyr).					
30171958	10	35	theme	time	1377:1380	arg1	PT					1383:1384	PT	1383:1384	PT	1383:1384	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	35	theme	time	1377:1380	arg1	time					1396:1399	prothrombin time (PT) clotting time	1365:1399	prothrombin time (PT) clotting time	1365:1399	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	12	36	theme	polysaccharides	1640:1654	arg1	activities					1619:1628	the immunomodulatory and anticoagulant activities	1580:1628	the immunomodulatory and anticoagulant activities of jujuba polysaccharides	1580:1654	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	8	37	theme	19.87:2.07:1.77:1.65:1.16	999:1023	arg1	ratio					990:994	a molar ratio	982:994	a molar ratio of 19.87:2.07:1.77:1.65:1.16	982:1023	JCS-2 was composed of GalA, mannose (Man), rhamnose (Rha), Ara and Gal, at a molar ratio of 19.87:2.07:1.77:1.65:1.16.					
30171958	7	38	theme	galactose	828:836	arg1	Ara					859:861	Ara	859:861	Ara	859:861	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	38	theme	galactose	828:836	arg1	Gal					839:841	Gal	839:841	Gal	839:841	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	38	theme	galactose	828:836	arg1	arabinose					848:856	arabinose	848:856	arabinose (Ara)	848:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	38	theme	galactose	828:836	arg1	galactose					828:836	galactose	828:836	galactose (Gal)	828:842	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	38	theme	galactose	828:836	arg1	amount					818:823	a small amount	810:823	a small amount of galactose (Gal) and arabinose (Ara)	810:862	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	12	39	theme	effective	1550:1558	arg1	method					1560:1565	an effective method	1547:1565	an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides	1547:1654	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	7	40	theme	molar	875:879	arg1	ratio					881:885	their molar ratio	869:885	their molar ratio	869:885	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	2	41	theme	Cellulose	187:195	arg1	chromatography					197:210	DEAE-52 Cellulose chromatography	179:210	DEAE-52 Cellulose chromatography	179:210	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	2	42	dep	polysaccharides	117:131	arg1	polysaccharides					117:131	Two polysaccharides	113:131	Two polysaccharides JCS-1 and JCS-2	113:147	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	2	42	dep	polysaccharides	117:131	arg1	JCS-2					143:147	JCS-2	143:147	JCS-2	143:147	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	2	42	dep	polysaccharides	117:131	arg1	JCS-1					133:137	JCS-1	133:137	JCS-1	133:137	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	2	43	theme	DEAE-52	179:185	arg1	chromatography					197:210	DEAE-52 Cellulose chromatography	179:210	DEAE-52 Cellulose chromatography	179:210	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	10	44	dep	time	1296:1299	arg1	times					1340:1344	clotting times	1331:1344	clotting times	1331:1344	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	0	45	theme	polysaccharides	51:65	arg1	modification					9:20	Sulfated modification	0:20	Sulfated modification	0:20	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	0	45	theme	polysaccharides	51:65	arg1	activities					37:46	biological activities	26:46	biological activities	26:46	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	7	46	contain	has	750:752	arg2	composition					779:789	galacturonic acid (GalA) composition	754:789	galacturonic acid (GalA) composition	754:789	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	7	46	contain	has	750:752	arg1	JCS-1					737:741	JCS-1	737:741	JCS-1	737:741	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	4	47	theme	nuclear	387:393	arg1	NMR					415:417	NMR	415:417	NMR	415:417	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	47	theme	nuclear	387:393	arg1	resonance					404:412	nuclear magnetic resonance	387:412	nuclear magnetic resonance (NMR)	387:418	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	11	48	theme	polysaccharides	1432:1446	arg1	substitution					1416:1427	substitution	1416:1427	substitution of polysaccharides	1416:1446	The degree of substitution of polysaccharides seemed to be closely related to their biological characteristics.					
30171958	10	49	theme	clotting	1331:1338	arg1	times					1340:1344	clotting times	1331:1344	clotting times	1331:1344	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	12	50	theme	anticoagulant	1605:1617	arg1	activities					1619:1628	the immunomodulatory and anticoagulant activities	1580:1628	the immunomodulatory and anticoagulant activities of jujuba polysaccharides	1580:1654	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	10	51	theme	thrombin	1312:1319	arg1	time					1321:1324	thrombin time	1312:1324	thrombin time (TT)	1312:1329	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	10	51	theme	thrombin	1312:1319	arg1	TT					1327:1328	TT	1327:1328	TT	1327:1328	Moreover, the sulfated polysaccharides significantly prolonged partial thromboplastin time (APTT) and thrombin time (TT) clotting times, but did not alter prothrombin time (PT) clotting time.					
30171958	7	52	theme	GalA	773:776	arg1	composition					779:789	galacturonic acid (GalA) composition	754:789	galacturonic acid (GalA) composition	754:789	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	4	53	theme	FT-IR	453:457	arg1	spectroscopy					460:471	(FT-IR) spectroscopy	452:471	(FT-IR) spectroscopy	452:471	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	54	theme	permeation	352:361	arg1	chromatography					363:376	high-performance gel permeation chromatography	331:376	high-performance gel permeation chromatography (HPGPC)	331:384	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	54	theme	permeation	352:361	arg1	HPGPC					379:383	HPGPC	379:383	HPGPC	379:383	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	6	55	theme	71.75 kDa	697:705	arg1	weight					682:687	the average molecular weight	660:687	the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa	660:720	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	55	theme	71.75 kDa	697:705	arg1	Mw					690:691	Mw	690:691	Mw	690:691	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	4	56	theme	high	284:287	arg1	chromatography					308:321	high performance liquid chromatography	284:321	high performance liquid chromatography (HPLC)	284:328	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	56	theme	high	284:287	arg1	HPLC					324:327	HPLC	324:327	HPLC	324:327	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	11	57	theme	substitution	1416:1427	arg1	degree					1406:1411	The degree	1402:1411	The degree of substitution of polysaccharides	1402:1446	The degree of substitution of polysaccharides seemed to be closely related to their biological characteristics.					
30171958	11	57	theme	substitution	1416:1427	arg1	related					1469:1475	related	1469:1475	related	1469:1475	The degree of substitution of polysaccharides seemed to be closely related to their biological characteristics.					
30171958	0	58	theme	Zizyphus	80:87	arg1	cv					96:97	Zizyphus jujuba cv	80:97	Zizyphus jujuba cv	80:97	Sulfated modification and biological activities of polysaccharides derived from Zizyphus jujuba cv.					
30171958	2	59	theme	Zizyphus	217:224	arg1	cv					233:234	Zizyphus jujuba cv	217:234	Zizyphus jujuba cv	217:234	Two polysaccharides JCS-1 and JCS-2 were successfully purified by DEAE-52 Cellulose chromatography from Zizyphus jujuba cv.					
30171958	4	60	theme	gel	348:350	arg1	chromatography					363:376	high-performance gel permeation chromatography	331:376	high-performance gel permeation chromatography (HPGPC)	331:384	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	60	theme	gel	348:350	arg1	HPGPC					379:383	HPGPC	379:383	HPGPC	379:383	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	6	61	theme	molecular	672:680	arg1	weight					682:687	the average molecular weight	660:687	the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa	660:720	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	6	61	theme	molecular	672:680	arg1	Mw					690:691	Mw	690:691	Mw	690:691	Structural analysis revealed that both JCS-1 and JCS-2 were size uniform polysaccharides with the average molecular weight (Mw) of 71.75 kDa and 357.39 kDa, respectively.					
30171958	7	62	theme	acid	767:770	arg1	composition					779:789	galacturonic acid (GalA) composition	754:789	galacturonic acid (GalA) composition	754:789	JCS-1 mainly has galacturonic acid (GalA) composition, and also contains a small amount of galactose (Gal) and arabinose (Ara), and their molar ratio is 39.04:1.26:1.39.					
30171958	9	63	contain	have	1124:1127	arg1	polysaccharides					1070:1084	natural polysaccharides	1062:1084	natural polysaccharides	1062:1084	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	9	63	contain	have	1124:1127	arg1	polysaccharides					1108:1122	sulfated modified polysaccharides	1090:1122	sulfated modified polysaccharides	1090:1122	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	9	63	contain	have	1124:1127	arg2	activity					1146:1153	immunomodulatory activity	1129:1153	immunomodulatory activity	1129:1153	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	9	64	theme	modified	1099:1106	arg1	polysaccharides					1108:1122	sulfated modified polysaccharides	1090:1122	sulfated modified polysaccharides	1090:1122	Activity studies indicate that both natural polysaccharides and sulfated modified polysaccharides have immunomodulatory activity, while sulfated modified polysaccharides are stronger.					
30171958	12	65	theme	Sulfated	1514:1521	arg1	modification					1523:1534	Sulfated modification	1514:1534	Sulfated modification	1514:1534	Sulfated modification represents an effective method of enhancing the immunomodulatory and anticoagulant activities of jujuba polysaccharides.					
30171958	4	66	dep	fourier	425:431	arg1	transform					433:441	transform	433:441	transform infrared (FT-IR) spectroscopy	433:471	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30171958	4	67	dep	transform	433:441	arg1	infrared					443:450	infrared	443:450	transform infrared (FT-IR) spectroscopy	433:471	Their structures were analyzed by high performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), nuclear magnetic resonance (NMR), and fourier transform infrared (FT-IR) spectroscopy.					
30011781	0	0	theme	Structural	102:111	arg1	Characterization					113:128	Structural Characterization	102:128	Structural Characterization	102:128	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	1	1	theme	one-factor-at-a-time	363:382	arg1	designs					422:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	4	2	theme	monosaccharide	962:975	arg1	compositions					977:988	the monosaccharide compositions	958:988	the monosaccharide compositions	958:988	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	1	3	theme	volvacea	293:300	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	4	theme	ultrasound	525:534	arg1	power					536:540	ultrasound power	525:540	ultrasound power of 175 W	525:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	0	5	from	Extraction	20:29	arg1	volvacea					67:74	Volvariella volvacea	55:74	Volvariella volvacea	55:74	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	2	6	theme	power	536:540	arg1	time					596:599	extraction time	585:599	extraction time of 33 min	585:609	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	6	theme	power	536:540	arg1	conditions					511:520	the optimized conditions	497:520	the optimized conditions of ultrasound power of 175 W	497:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	6	theme	power	536:540	arg1	ratio					620:624	the ratio	616:624	the ratio of liquid to raw material of 25:1	616:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	6	theme	power	536:540	arg1	temperature					563:573	extraction temperature	552:573	extraction temperature of 57 °C	552:582	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	4	7	theme	ultrasound	928:937	arg1	treatment					939:947	ultrasound treatment	928:947	ultrasound treatment	928:947	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	4	8	theme	weight	1016:1021	arg1	range					1023:1027	molecular weight range	1006:1027	molecular weight range of polysaccharides extracted from V. volvacea	1006:1073	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	1	9	theme	three-level	388:398	arg1	designs					422:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	3	10	from	volvacea	789:796	arg1	polysaccharides					765:779	polysaccharides	765:779	polysaccharides from V. volvacea	765:796	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	3	10	from	volvacea	789:796	arg1	extraction					751:760	the extraction	747:760	the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency	747:847	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	1	11	theme	straw	303:307	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	12	theme	extraction	552:561	arg1	temperature					563:573	extraction temperature	552:573	extraction temperature of 57 °C	552:582	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	1	13	theme	Box-Behnken	400:410	arg1	designs					422:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	14	theme	min	607:609	arg1	time					596:599	extraction time	585:599	extraction time of 33 min	585:609	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	14	theme	min	607:609	arg1	conditions					511:520	the optimized conditions	497:520	the optimized conditions of ultrasound power of 175 W	497:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	14	theme	min	607:609	arg1	ratio					620:624	the ratio	616:624	the ratio of liquid to raw material of 25:1	616:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	14	theme	min	607:609	arg1	temperature					563:573	extraction temperature	552:573	extraction temperature of 57 °C	552:582	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	1	15	theme	mushroom	309:316	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	16	theme	factorial	412:420	arg1	designs					422:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	one-factor-at-a-time and three-level Box-Behnken factorial designs	363:428	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	17	from	bodies	328:333	arg1	yield					221:225	the yield	217:225	the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies	217:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	17	from	bodies	328:333	arg1	extraction					265:274	ultrasound-assisted polysaccharide extraction	230:274	ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies	230:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	18	theme	raw	639:641	arg1	material					643:650	raw material	639:650	raw material of 25:1	639:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	1	19	theme	operational	182:192	arg1	parameters					194:203	the operational parameters	178:203	the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs	178:428	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	20	theme	W	549:549	arg1	power					536:540	ultrasound power	525:540	ultrasound power of 175 W	525:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	21	theme	liquid	629:634	arg1	time					596:599	extraction time	585:599	extraction time of 33 min	585:609	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	21	theme	liquid	629:634	arg1	conditions					511:520	the optimized conditions	497:520	the optimized conditions of ultrasound power of 175 W	497:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	21	theme	liquid	629:634	arg1	ratio					620:624	the ratio	616:624	the ratio of liquid to raw material of 25:1	616:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	21	theme	liquid	629:634	arg1	temperature					563:573	extraction temperature	552:573	extraction temperature of 57 °C	552:582	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	3	22	theme	V.	786:787	arg1	volvacea					789:796	V. volvacea	786:796	V. volvacea	786:796	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	2	23	theme	polysaccharide	441:454	arg1	yield					456:460	A maximum polysaccharide yield	431:460	A maximum polysaccharide yield of 8.28 ± 0.23%	431:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	0	24	from	volvacea	67:74	arg1	Polysaccharides					34:48	Polysaccharides	34:48	Polysaccharides from Volvariella volvacea	34:74	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	0	24	from	volvacea	67:74	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.	0:129	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	2	25	theme	maximum	433:439	arg1	yield					456:460	A maximum polysaccharide yield	431:460	A maximum polysaccharide yield of 8.28 ± 0.23%	431:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	0	26	theme	Ultrasound-Assisted	0:18	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.	0:129	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	4	27	theme	structural	885:894	arg1	characterization					896:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	4	28	theme	polysaccharide	870:883	arg1	characterization					896:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	0	29	theme	Polysaccharides	34:48	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.	0:129	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	1	30	theme	fruiting	319:326	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	31	theme	25:1	655:658	arg1	material					643:650	raw material	639:650	raw material of 25:1	639:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	32	theme	0.23	472:475	arg1	%					476:476	%	476:476	%	476:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	33	theme	%	476:476	arg1	±					470:470	8.28 ±	465:470	8.28 ± 0.23%	465:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	34	theme	°C	581:582	arg1	time					596:599	extraction time	585:599	extraction time of 33 min	585:609	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	34	theme	°C	581:582	arg1	conditions					511:520	the optimized conditions	497:520	the optimized conditions of ultrasound power of 175 W	497:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	34	theme	°C	581:582	arg1	ratio					620:624	the ratio	616:624	the ratio of liquid to raw material of 25:1	616:658	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	34	theme	°C	581:582	arg1	temperature					563:573	extraction temperature	552:573	extraction temperature of 57 °C	552:582	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	2	35	theme	±	470:470	arg1	yield					456:460	A maximum polysaccharide yield	431:460	A maximum polysaccharide yield of 8.28 ± 0.23%	431:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	0	36	theme	Volvariella	55:65	arg1	volvacea					67:74	Volvariella volvacea	55:74	Volvariella volvacea	55:74	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	4	37	theme	molecular	1006:1014	arg1	range					1023:1027	molecular weight range	1006:1027	molecular weight range of polysaccharides extracted from V. volvacea	1006:1073	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	2	38	theme	8.28	465:468	arg1	±					470:470	8.28 ±	465:470	8.28 ± 0.23%	465:476	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	1	39	theme	ultrasound-assisted	230:248	arg1	extraction					265:274	ultrasound-assisted polysaccharide extraction	230:274	ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies	230:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	40	theme	optimized	501:509	arg1	conditions					511:520	the optimized conditions	497:520	the optimized conditions of ultrasound power of 175 W	497:549	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	3	41	theme	polysaccharides	765:779	arg1	extraction					751:760	the extraction	747:760	the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency	747:847	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	4	42	theme	Further	850:856	arg1	characterization					896:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	4	43	theme	preliminary	858:868	arg1	characterization					896:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization	850:911	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	3	44	from	extraction	751:760	arg1	volvacea					789:796	V. volvacea	786:796	V. volvacea	786:796	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	1	45	theme	first	352:356	arg1	time					358:361	the first time	348:361	the first time	348:361	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	46	from	yield	221:225	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	47	theme	present	147:153	arg1	study					155:159	the present study	143:159	the present study	143:159	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	48	theme	polysaccharide	250:263	arg1	extraction					265:274	ultrasound-assisted polysaccharide extraction	230:274	ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies	230:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	2	49	theme	extraction	585:594	arg1	time					596:599	extraction time	585:599	extraction time of 33 min	585:609	A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively.					
30011781	1	50	theme	study	155:159	arg1	aims					135:138	The aims	131:138	The aims of the present study	131:159	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	1	51	theme	extraction	265:274	arg1	yield					221:225	the yield	217:225	the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies	217:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
30011781	0	52	dep	Extraction	20:29	arg1	Optimization					85:96	Process Optimization	77:96	Process Optimization	77:96	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	0	52	dep	Extraction	20:29	arg1	Characterization					113:128	Structural Characterization	102:128	Structural Characterization	102:128	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	4	53	theme	polysaccharides	1032:1046	arg1	range					1023:1027	molecular weight range	1006:1027	molecular weight range of polysaccharides extracted from V. volvacea	1006:1073	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	4	53	theme	polysaccharides	1032:1046	arg1	ratios					994:999	ratios	994:999	ratios	994:999	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	4	53	theme	polysaccharides	1032:1046	arg1	compositions					977:988	the monosaccharide compositions	958:988	the monosaccharide compositions	958:988	Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.					
30011781	0	54	theme	Process	77:83	arg1	Optimization					85:96	Process Optimization	77:96	Process Optimization	77:96	Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization.					
30011781	3	55	theme	hot-water	691:699	arg1	extraction					701:710	the hot-water extraction	687:710	the hot-water extraction	687:710	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	3	56	theme	higher	806:811	arg1	yield					828:832	its higher polysaccharide yield	802:832	its higher polysaccharide yield	802:832	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	3	57	theme	polysaccharide	813:826	arg1	yield					828:832	its higher polysaccharide yield	802:832	its higher polysaccharide yield	802:832	Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency.					
30011781	1	58	theme	Volvariella	281:291	arg1	bodies					328:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	Volvariella volvacea (straw mushroom) fruiting bodies	281:333	The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs.					
32247498	7	0	theme	complex	828:834	arg1	profile					836:842	a heterogeneous and complex profile	808:842	a heterogeneous and complex profile	808:842	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	7	0	theme	complex	828:834	arg1	as					790:791	as	790:791	as	790:791	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	10	1	theme	complex	1137:1143	arg1	xylose					1180:1185	xylose	1180:1185	mainly xylose	1173:1185	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	10	1	theme	complex	1137:1143	arg1	mixture					1145:1151	a complex mixture	1135:1151	a complex mixture of monosaccharides	1135:1170	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	1	2	theme	phenolic	177:184	arg1	compounds					186:194	phenolic compounds	177:194	phenolic compounds	177:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	11	3	from	effect	1380:1385	arg1	viability					1395:1403	cell viability	1390:1403	cell viability	1390:1403	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	4	from	100 μg/mL	1264:1272	arg1	one					1217:1219	one	1217:1219	one	1217:1219	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	4	from	100 μg/mL	1264:1272	arg1	PRF					1200:1202	This latter PRF	1188:1202	This latter PRF	1188:1202	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	5	theme	NF-κB	1326:1330	arg1	release					1291:1297	the release	1287:1297	the release of TNF-α	1287:1306	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	5	theme	NF-κB	1326:1330	arg1	activation					1312:1321	activation	1312:1321	activation of NF-κB in LPS-activated RAW 264.7 macrophages	1312:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	14	6	theme	RG	1946:1947	arg1	II					1949:1950	possible RG I and RG II and xylan chains	1928:1967	II	1949:1950	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	1	7	theme	compounds	186:194	arg1	source					167:172	a well recognized source	149:172	a well recognized source of phenolic compounds	149:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	7	theme	compounds	186:194	arg1	by-products					136:146	by-products	136:146	by-products	136:146	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	14	8	theme	bioactive	1769:1777	arg1	fractions					1779:1787	bioactive fractions	1769:1787	bioactive fractions	1769:1787	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	12	9	with	polysaccharides	1520:1534	arg1	application					1551:1561	potential application	1541:1561	potential application	1541:1561	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	4	10	theme	monosaccharide	528:541	arg1	composition					543:553	their monosaccharide composition	522:553	their monosaccharide composition	522:553	PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight.					
32247498	12	11	theme	compounds	1596:1604	arg1	polysaccharides					1520:1534	bioactive polysaccharides	1510:1534	bioactive polysaccharides with potential application	1510:1561	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	12	11	theme	compounds	1596:1604	arg1	source					1568:1573	a source	1566:1573	a source of anti-inflammatory compounds	1566:1604	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	5	12	theme	PRFs	641:644	arg1	activity					629:636	Anti-inflammatory activity	611:636	Anti-inflammatory activity of PRFs	611:644	Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages.					
32247498	2	13	theme	techniques	306:315	arg1	PRF					272:274	PRF	272:274	PRF	272:274	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	2	13	theme	techniques	306:315	arg1	effects					281:287	effects	281:287	effects of fractionation techniques on its potential bioactivity	281:344	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	2	13	theme	techniques	306:315	arg1	fraction					262:269	the polysaccharide-rich fraction	238:269	the polysaccharide-rich fraction (PRF)	238:275	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	12	14	theme	anti-inflammatory	1578:1594	arg1	compounds					1596:1604	anti-inflammatory compounds	1578:1604	anti-inflammatory compounds	1578:1604	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	7	15	located	detected	848:855	arg2	profile					836:842	a heterogeneous and complex profile	808:842	a heterogeneous and complex profile	808:842	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	7	15	located	detected	848:855	arg1	extracts					860:867	extracts	860:867	extracts procured under alkaline conditions	860:902	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	7	15	located	detected	848:855	arg2	as					790:791	as	790:791	as	790:791	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	10	16	theme	monosaccharides	1156:1170	arg1	xylose					1180:1185	xylose	1180:1185	mainly xylose	1173:1185	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	10	16	theme	monosaccharides	1156:1170	arg1	mixture					1145:1151	a complex mixture	1135:1151	a complex mixture of monosaccharides	1135:1170	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	12	17	theme	grape	1424:1428	arg1	variety					1430:1436	the grape variety	1420:1436	the grape variety	1420:1436	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	2	18	theme	fractionation	292:304	arg1	techniques					306:315	fractionation techniques	292:315	fractionation techniques	292:315	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	6	19	theme	heterogeneous	758:770	arg1	profiles					772:779	heterogeneous profiles	758:779	heterogeneous profiles	758:779	PRF obtained in water and/or under acidic conditions showed heterogeneous profiles.					
32247498	11	20	theme	latter	1193:1198	arg1	one					1217:1219	one	1217:1219	one	1217:1219	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	20	theme	latter	1193:1198	arg1	PRF					1200:1202	This latter PRF	1188:1202	This latter PRF	1188:1202	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	21	theme	RAW	1349:1351	arg1	macrophages					1359:1369	LPS-activated RAW 264.7 macrophages	1335:1369	LPS-activated RAW 264.7 macrophages	1335:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	8	22	theme	high	907:910	arg1	content					912:918	A high content	905:918	A high content of uronic acid	905:933	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	9	23	located	present	1036:1042	arg1	PRFs					1047:1050	PRFs	1047:1050	PRFs procured under acidic conditions	1047:1083	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	23	located	present	1036:1042	arg2	hemicellulose					1017:1029	hemicellulose	1017:1029	hemicellulose	1017:1029	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	23	located	present	1036:1042	arg2	Pectin					1006:1011	Pectin	1006:1011	Pectin	1006:1011	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	8	24	located	found	939:943	arg1	extracts					956:963	aqueous extracts	948:963	aqueous extracts	948:963	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	8	24	located	found	939:943	arg2	content					912:918	A high content	905:918	A high content of uronic acid	905:933	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	5	25	theme	stimulated	664:673	arg1	macrophages					685:695	stimulated RAW 264.7 macrophages	664:695	stimulated RAW 264.7 macrophages	664:695	Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages.					
32247498	11	26	from	release	1291:1297	arg1	macrophages					1359:1369	LPS-activated RAW 264.7 macrophages	1335:1369	LPS-activated RAW 264.7 macrophages	1335:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	2	27	from	fraction	262:269	arg1	bioactivity					334:344	its potential bioactivity	320:344	its potential bioactivity	320:344	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	0	28	from	by-products	85:95	arg1	polysaccharides					53:67	anti-inflammatory polysaccharides	35:67	anti-inflammatory polysaccharides from winemaking by-products	35:95	Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.					
32247498	8	29	theme	uronic	923:928	arg1	acid					930:933	uronic acid	923:933	uronic acid	923:933	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	5	30	theme	RAW	675:677	arg1	macrophages					685:695	stimulated RAW 264.7 macrophages	664:695	stimulated RAW 264.7 macrophages	664:695	Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages.					
32247498	1	31	theme	large	119:123	arg1	amounts					125:131	large amounts	119:131	large amounts of by-products, a well recognized source of phenolic compounds	119:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	31	theme	large	119:123	arg1	source					167:172	a well recognized source	149:172	a well recognized source of phenolic compounds	149:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	31	theme	large	119:123	arg1	by-products					136:146	by-products	136:146	by-products	136:146	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	6	32	theme	acidic	733:738	arg1	conditions					740:749	acidic conditions	733:749	acidic conditions	733:749	PRF obtained in water and/or under acidic conditions showed heterogeneous profiles.					
32247498	3	33	theme	pH	451:452	arg1	aqueous					434:440	aqueous	434:440	aqueous	434:440	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	3	33	theme	pH	451:452	arg1	conditions					454:463	neutral pH conditions	443:463	neutral pH conditions	443:463	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	0	34	theme	Alkaline	0:7	arg1	conditions					9:18	Alkaline conditions	0:18	Alkaline conditions	0:18	Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.					
32247498	11	35	theme	LPS-activated	1335:1347	arg1	macrophages					1359:1369	LPS-activated RAW 264.7 macrophages	1335:1369	LPS-activated RAW 264.7 macrophages	1335:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	5	36	theme	Anti-inflammatory	611:627	arg1	activity					629:636	Anti-inflammatory activity	611:636	Anti-inflammatory activity of PRFs	611:644	Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages.					
32247498	2	37	theme	potential	324:332	arg1	bioactivity					334:344	its potential bioactivity	320:344	its potential bioactivity	320:344	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	3	38	theme	winemaking	390:399	arg1	by-products					401:411	winemaking by-products	390:411	winemaking by-products	390:411	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	14	39	theme	linked	1833:1838	arg1	chain					1807:1811	a chain	1805:1811	a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains	1805:1967	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	9	40	from	PRFs	1047:1050	arg1	present					1036:1042	present	1036:1042	present	1036:1042	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	8	41	theme	acid	930:933	arg1	content					912:918	A high content	905:918	A high content of uronic acid	905:933	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	3	42	theme	alkaline	478:485	arg1	aqueous					434:440	aqueous	434:440	aqueous	434:440	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	3	42	theme	alkaline	478:485	arg1	conditions					487:496	alkaline conditions	478:496	alkaline conditions	478:496	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	4	43	theme	molecular	593:601	arg1	weight					603:608	molecular weight	593:608	molecular weight	593:608	PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight.					
32247498	12	44	theme	potential	1541:1549	arg1	application					1551:1561	potential application	1541:1561	potential application	1541:1561	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	1	45	theme	by-products	136:146	arg1	amounts					125:131	large amounts	119:131	large amounts of by-products, a well recognized source of phenolic compounds	119:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	45	theme	by-products	136:146	arg1	source					167:172	a well recognized source	149:172	a well recognized source of phenolic compounds	149:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	45	theme	by-products	136:146	arg1	by-products					136:146	by-products	136:146	by-products	136:146	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	10	46	contain	containing	1124:1133	arg2	mixture					1145:1151	a complex mixture	1135:1151	a complex mixture of monosaccharides	1135:1170	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	10	46	contain	containing	1124:1133	arg1	extracts					1115:1122	extracts	1115:1122	extracts containing a complex mixture of monosaccharides, mainly xylose	1115:1185	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	10	46	contain	containing	1124:1133	arg2	xylose					1180:1185	xylose	1180:1185	mainly xylose	1173:1185	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	11	47	theme	cell	1390:1393	arg1	viability					1395:1403	cell viability	1390:1403	cell viability	1390:1403	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	3	48	from	Syrah	368:372	arg1	PRFs					358:361	PRFs	358:361	PRFs from Syrah and Tempranillo winemaking by-products	358:411	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	11	49	theme	TNF-α	1302:1306	arg1	release					1291:1297	the release	1287:1297	the release of TNF-α	1287:1306	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	11	49	theme	TNF-α	1302:1306	arg1	activation					1312:1321	activation	1312:1321	activation of NF-κB in LPS-activated RAW 264.7 macrophages	1312:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	0	50	theme	anti-inflammatory	35:51	arg1	polysaccharides					53:67	anti-inflammatory polysaccharides	35:67	anti-inflammatory polysaccharides from winemaking by-products	35:95	Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.					
32247498	4	51	theme	uronic	556:561	arg1	content					568:574	uronic acid content	556:574	uronic acid content	556:574	PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight.					
32247498	3	52	theme	neutral	443:449	arg1	aqueous					434:440	aqueous	434:440	aqueous	434:440	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	3	52	theme	neutral	443:449	arg1	conditions					454:463	neutral pH conditions	443:463	neutral pH conditions	443:463	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	2	53	theme	polysaccharide-rich	242:260	arg1	PRF					272:274	PRF	272:274	PRF	272:274	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	2	53	theme	polysaccharide-rich	242:260	arg1	fraction					262:269	the polysaccharide-rich fraction	238:269	the polysaccharide-rich fraction (PRF)	238:275	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	14	54	theme	RG	1937:1938	arg1	I					1940:1940	possible RG I and RG II and xylan chains	1928:1967	I	1940:1940	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	0	55	theme	winemaking	74:83	arg1	by-products					85:95	winemaking by-products	74:95	winemaking by-products	74:95	Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.					
32247498	7	56	theme	heterogeneous	810:822	arg1	profile					836:842	a heterogeneous and complex profile	808:842	a heterogeneous and complex profile	808:842	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	7	56	theme	heterogeneous	810:822	arg1	as					790:791	as	790:791	as	790:791	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	13	57	theme	anti-inflammatory	1664:1680	arg1	effects					1682:1688	the anti-inflammatory effects	1660:1688	the anti-inflammatory effects	1660:1688	A complex mixture of polymers may be responsible for the anti-inflammatory effects.					
32247498	3	58	from	Tempranillo	378:388	arg1	PRFs					358:361	PRFs	358:361	PRFs from Syrah and Tempranillo winemaking by-products	358:411	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	14	59	theme	possible	1928:1935	arg1	I					1940:1940	possible RG I and RG II and xylan chains	1928:1967	I	1940:1940	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	8	60	theme	aqueous	948:954	arg1	extracts					956:963	aqueous extracts	948:963	aqueous extracts	948:963	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	11	61	from	activation	1312:1321	arg1	macrophages					1359:1369	LPS-activated RAW 264.7 macrophages	1335:1369	LPS-activated RAW 264.7 macrophages	1335:1369	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
32247498	12	62	theme	best	1488:1491	arg1	PRFs					1439:1442	PRFs	1439:1442	PRFs obtained under alkaline conditions	1439:1477	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	12	62	theme	best	1488:1491	arg1	option					1493:1498	the best option	1484:1498	the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds	1484:1604	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	3	63	dep	Syrah	368:372	arg1	by-products					401:411	winemaking by-products	390:411	winemaking by-products	390:411	Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions.					
32247498	13	64	theme	complex	1609:1615	arg1	responsible					1644:1654	responsible	1644:1654	responsible	1644:1654	A complex mixture of polymers may be responsible for the anti-inflammatory effects.					
32247498	13	64	theme	complex	1609:1615	arg1	mixture					1617:1623	A complex mixture	1607:1623	A complex mixture of polymers	1607:1635	A complex mixture of polymers may be responsible for the anti-inflammatory effects.					
32247498	13	65	theme	polymers	1628:1635	arg1	responsible					1644:1654	responsible	1644:1654	responsible	1644:1654	A complex mixture of polymers may be responsible for the anti-inflammatory effects.					
32247498	13	65	theme	polymers	1628:1635	arg1	mixture					1617:1623	A complex mixture	1607:1623	A complex mixture of polymers	1607:1635	A complex mixture of polymers may be responsible for the anti-inflammatory effects.					
32247498	4	66	theme	acid	563:566	arg1	content					568:574	uronic acid content	556:574	uronic acid content	556:574	PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight.					
32247498	12	67	theme	alkaline	1459:1466	arg1	conditions					1468:1477	alkaline conditions	1459:1477	alkaline conditions	1459:1477	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	10	68	theme	Alkaline	1086:1093	arg1	conditions					1095:1104	Alkaline conditions	1086:1104	Alkaline conditions	1086:1104	Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose.					
32247498	8	69	theme	pectin	998:1003	arg1	presence					986:993	the presence	982:993	the presence of pectin	982:1003	A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin.					
32247498	2	70	from	effects	281:287	arg1	bioactivity					334:344	its potential bioactivity	320:344	its potential bioactivity	320:344	However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity.					
32247498	14	71	theme	xylan	1956:1960	arg1	chains					1962:1967	possible RG I and RG II and xylan chains	1928:1967	chains	1962:1967	Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of α-L-Araf-(1 → 3) linked, β-D-Xylp- (1 → 4), α-D-Glcp-(1 → 4) linked, α-D-GalpA-(1 → 4), α-D-Gal-(1 → 2) forming possible RG I and RG II and xylan chains.					
32247498	1	72	theme	recognized	156:165	arg1	source					167:172	a well recognized source	149:172	a well recognized source of phenolic compounds	149:194	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	1	72	theme	recognized	156:165	arg1	by-products					136:146	by-products	136:146	by-products	136:146	Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds.					
32247498	7	73	theme	alkaline	884:891	arg1	conditions					893:902	alkaline conditions	884:902	alkaline conditions	884:902	As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions.					
32247498	12	74	theme	bioactive	1510:1518	arg1	polysaccharides					1520:1534	bioactive polysaccharides	1510:1534	bioactive polysaccharides with potential application	1510:1561	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	12	74	theme	bioactive	1510:1518	arg1	source					1568:1573	a source	1566:1573	a source of anti-inflammatory compounds	1566:1604	Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds.					
32247498	9	75	theme	acidic	1067:1072	arg1	conditions					1074:1083	acidic conditions	1067:1083	acidic conditions	1067:1083	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	76	from	present	1036:1042	arg1	PRFs					1047:1050	PRFs	1047:1050	PRFs procured under acidic conditions	1047:1083	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	77	attach	present	1036:1042	arg1	PRFs					1047:1050	PRFs	1047:1050	PRFs procured under acidic conditions	1047:1083	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	77	attach	present	1036:1042	arg2	hemicellulose					1017:1029	hemicellulose	1017:1029	hemicellulose	1017:1029	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	9	77	attach	present	1036:1042	arg2	Pectin					1006:1011	Pectin	1006:1011	Pectin	1006:1011	Pectin and hemicellulose were present in PRFs procured under acidic conditions.					
32247498	11	78	theme	anti-inflammatory	1232:1248	arg1	potential					1250:1258	anti-inflammatory potential	1232:1258	anti-inflammatory potential	1232:1258	This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 μg/mL) by reducing the release of TNF-α and activation of NF-κB in LPS-activated RAW 264.7 macrophages, with no effect on cell viability.					
29691034	5	0	theme	test	749:752	arg1	BPS					690:692	BPS	690:692	BPS	690:692	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	5	0	theme	test	749:752	arg1	drug					754:757	a test drug	747:757	a test drug	747:757	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	1	1	theme	bacteria	176:183	arg1	atrophaeus					242:251	Bacillus atrophaeus	233:251	Bacillus atrophaeus	233:251	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	1	theme	bacteria	176:183	arg1	yield					148:152	A high yield	141:152	A high yield of exopolysaccharides bacteria isolated from mangrove system	141:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	1	theme	bacteria	176:183	arg1	strain					282:287	WYZ strain	278:287	WYZ strain	278:287	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	6	2	theme	transdermal	974:984	arg1	effect					1007:1012	transdermal absorption promoting effect	974:1012	transdermal absorption promoting effect	974:1012	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	6	3	contain	has	1047:1049	arg1	BPS					1043:1045	BPS	1043:1045	BPS	1043:1045	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	6	3	contain	has	1047:1049	arg2	potential					1057:1065	great potential	1051:1065	great potential	1051:1065	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	5	4	theme	transdermal	875:885	arg1	absorption					887:896	lidocaine transdermal absorption	865:896	lidocaine transdermal absorption	865:896	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	0	5	theme	atrophaeus	118:127	arg1	strain					133:138	Bacillus atrophaeus WYZ strain	109:138	Bacillus atrophaeus WYZ strain	109:138	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	3	6	theme	intrinsic	523:531	arg1	viscosity					533:541	intrinsic viscosity	523:541	intrinsic viscosity	523:541	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	1	7	theme	WYZ	278:280	arg1	yield					148:152	A high yield	141:152	A high yield of exopolysaccharides bacteria isolated from mangrove system	141:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	7	theme	WYZ	278:280	arg1	strain					282:287	WYZ strain	278:287	WYZ strain	278:287	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	0	8	theme	Bacillus	109:116	arg1	strain					133:138	Bacillus atrophaeus WYZ strain	109:138	Bacillus atrophaeus WYZ strain	109:138	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	6	9	theme	retention	951:959	arg1	capacity					961:968	good water retention capacity	940:968	good water retention capacity	940:968	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	5	10	theme	lidocaine	865:873	arg1	absorption					887:896	lidocaine transdermal absorption	865:896	lidocaine transdermal absorption	865:896	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	3	11	theme	molecular	472:480	arg1	weight					482:487	weight average molecular weight	457:487	weight average molecular weight (Mw)	457:492	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	3	11	theme	molecular	472:480	arg1	Mw					490:491	Mw	490:491	Mw	490:491	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	6	12	theme	great	1051:1055	arg1	potential					1057:1065	great potential	1051:1065	great potential	1051:1065	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	2	13	theme	0.58 g/L	388:395	arg1	yield					373:377	a yield	371:377	a yield of about 0.58 g/L	371:395	An exopolysaccharide (BPS) was obtained from this strain after purification with a yield of about 0.58 g/L.					
29691034	4	14	theme	wound	638:642	arg1	structure					673:681	porous wound three-dimensional spider web structure	631:681	porous wound three-dimensional spider web structure	631:681	The microstructure (SEM) showed that BPS was porous wound three-dimensional spider web structure.					
29691034	0	15	theme	WYZ	129:131	arg1	strain					133:138	Bacillus atrophaeus WYZ strain	109:138	Bacillus atrophaeus WYZ strain	109:138	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	1	16	attach	isolated	185:192	arg1	system					208:213	mangrove system	199:213	mangrove system	199:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	16	attach	isolated	185:192	arg2	bacteria					176:183	exopolysaccharides bacteria	157:183	exopolysaccharides bacteria isolated from mangrove system	157:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	3	17	theme	physical	408:415	arg1	properties					430:439	some physical and chemical properties	403:439	some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity	403:570	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	5	18	theme	anesthesia	779:788	arg1	experiments					806:816	in vitro and local anesthesia in live animals experiments	760:816	in vitro and local anesthesia in live animals experiments	760:816	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	3	19	theme	BPS	444:446	arg1	properties					430:439	some physical and chemical properties	403:439	some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity	403:570	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	5	20	theme	local	773:777	arg1	anesthesia					779:788	local anesthesia	773:788	local anesthesia in live animals	773:804	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	5	21	from	anesthesia	779:788	arg1	animals					798:804	live animals	793:804	live animals	793:804	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	4	22	theme	porous	631:636	arg1	structure					673:681	porous wound three-dimensional spider web structure	631:681	porous wound three-dimensional spider web structure	631:681	The microstructure (SEM) showed that BPS was porous wound three-dimensional spider web structure.					
29691034	3	23	theme	retention	553:561	arg1	capacity					563:570	water retention capacity	547:570	water retention capacity	547:570	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	3	24	theme	chemical	421:428	arg1	properties					430:439	some physical and chemical properties	403:439	some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity	403:570	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	0	25	theme	Physical	0:7	arg1	properties					22:31	Physical and chemical properties	0:31	Physical and chemical properties	0:31	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	0	25	theme	Physical	0:7	arg1	effects					68:74	percutaneous absorption-promoting effects	34:74	percutaneous absorption-promoting effects of exopolysaccharide	34:95	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	6	26	theme	water	945:949	arg1	capacity					961:968	good water retention capacity	940:968	good water retention capacity	940:968	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	5	27	dep	in	760:761	arg1	vitro					763:767	vitro	763:767	vitro	763:767	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	4	28	theme	three-dimensional	644:660	arg1	structure					673:681	porous wound three-dimensional spider web structure	631:681	porous wound three-dimensional spider web structure	631:681	The microstructure (SEM) showed that BPS was porous wound three-dimensional spider web structure.					
29691034	6	29	theme	good	940:943	arg1	capacity					961:968	good water retention capacity	940:968	good water retention capacity	940:968	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	0	30	theme	chemical	13:20	arg1	properties					22:31	Physical and chemical properties	0:31	Physical and chemical properties	0:31	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	0	30	theme	chemical	13:20	arg1	effects					68:74	percutaneous absorption-promoting effects	34:74	percutaneous absorption-promoting effects of exopolysaccharide	34:95	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	3	31	theme	water	547:551	arg1	capacity					563:570	water retention capacity	547:570	water retention capacity	547:570	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	3	32	theme	weight	457:462	arg1	weight					482:487	weight average molecular weight	457:487	weight average molecular weight (Mw)	457:492	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	3	32	theme	weight	457:462	arg1	Mw					490:491	Mw	490:491	Mw	490:491	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	0	33	theme	percutaneous	34:45	arg1	properties					22:31	Physical and chemical properties	0:31	Physical and chemical properties	0:31	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	0	33	theme	percutaneous	34:45	arg1	effects					68:74	percutaneous absorption-promoting effects	34:74	percutaneous absorption-promoting effects of exopolysaccharide	34:95	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	4	34	theme	spider	662:667	arg1	structure					673:681	porous wound three-dimensional spider web structure	631:681	porous wound three-dimensional spider web structure	631:681	The microstructure (SEM) showed that BPS was porous wound three-dimensional spider web structure.					
29691034	5	35	theme	transdermal	697:707	arg1	enhancer					720:727	transdermal absorption enhancer	697:727	transdermal absorption enhancer	697:727	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	5	36	theme	in	760:761	arg1	experiments					806:816	in vitro and local anesthesia in live animals experiments	760:816	in vitro and local anesthesia in live animals experiments	760:816	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	5	37	theme	absorption	709:718	arg1	enhancer					720:727	transdermal absorption enhancer	697:727	transdermal absorption enhancer	697:727	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	6	38	contain	had	936:938	arg2	effect					1007:1012	transdermal absorption promoting effect	974:1012	transdermal absorption promoting effect	974:1012	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	6	38	contain	had	936:938	arg2	capacity					961:968	good water retention capacity	940:968	good water retention capacity	940:968	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	6	38	contain	had	936:938	arg1	BPS					932:934	the BPS	928:934	the BPS	928:934	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	6	39	theme	biomaterials	1083:1094	arg1	field					1074:1078	the field	1070:1078	the field of biomaterials	1070:1094	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	0	40	theme	absorption-promoting	47:66	arg1	properties					22:31	Physical and chemical properties	0:31	Physical and chemical properties	0:31	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	0	40	theme	absorption-promoting	47:66	arg1	effects					68:74	percutaneous absorption-promoting effects	34:74	percutaneous absorption-promoting effects of exopolysaccharide	34:95	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	2	41	with	purification	353:364	arg1	yield					373:377	a yield	371:377	a yield of about 0.58 g/L	371:395	An exopolysaccharide (BPS) was obtained from this strain after purification with a yield of about 0.58 g/L.					
29691034	4	42	theme	web	669:671	arg1	structure					673:681	porous wound three-dimensional spider web structure	631:681	porous wound three-dimensional spider web structure	631:681	The microstructure (SEM) showed that BPS was porous wound three-dimensional spider web structure.					
29691034	1	43	theme	high	143:146	arg1	atrophaeus					242:251	Bacillus atrophaeus	233:251	Bacillus atrophaeus	233:251	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	43	theme	high	143:146	arg1	yield					148:152	A high yield	141:152	A high yield of exopolysaccharides bacteria isolated from mangrove system	141:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	43	theme	high	143:146	arg1	strain					282:287	WYZ strain	278:287	WYZ strain	278:287	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	1	44	theme	exopolysaccharides	157:174	arg1	bacteria					176:183	exopolysaccharides bacteria	157:183	exopolysaccharides bacteria isolated from mangrove system	157:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	3	45	theme	average	464:470	arg1	weight					482:487	weight average molecular weight	457:487	weight average molecular weight (Mw)	457:492	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	3	45	theme	average	464:470	arg1	Mw					490:491	Mw	490:491	Mw	490:491	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	0	46	theme	exopolysaccharide	79:95	arg1	properties					22:31	Physical and chemical properties	0:31	Physical and chemical properties	0:31	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	0	46	theme	exopolysaccharide	79:95	arg1	effects					68:74	percutaneous absorption-promoting effects	34:74	percutaneous absorption-promoting effects of exopolysaccharide	34:95	Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain.					
29691034	1	47	theme	mangrove	199:206	arg1	system					208:213	mangrove system	199:213	mangrove system	199:213	A high yield of exopolysaccharides bacteria isolated from mangrove system was identified as Bacillus atrophaeus by 16S rDNA and named as WYZ strain.					
29691034	6	48	theme	promoting	997:1005	arg1	effect					1007:1012	transdermal absorption promoting effect	974:1012	transdermal absorption promoting effect	974:1012	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29691034	5	49	theme	live	793:796	arg1	animals					798:804	live animals	793:804	live animals	793:804	Using BPS as transdermal absorption enhancer, and lidocaine as a test drug, in vitro and local anesthesia in live animals experiments were conducted to explore that the BPS promote lidocaine transdermal absorption and mechanism.					
29691034	3	50	theme	monosaccharide	495:508	arg1	composition					510:520	monosaccharide composition	495:520	monosaccharide composition	495:520	Then some physical and chemical properties of BPS, such as weight average molecular weight (Mw), monosaccharide composition, intrinsic viscosity and water retention capacity were studied.					
29691034	6	51	theme	absorption	986:995	arg1	effect					1007:1012	transdermal absorption promoting effect	974:1012	transdermal absorption promoting effect	974:1012	In conclusion, the BPS had good water retention capacity and transdermal absorption promoting effect, all of these indicated that BPS has great potential in the field of biomaterials.					
29455975	4	0	theme	monosaccharides	810:824	arg1	composition					826:836	similar monosaccharides composition	802:836	similar monosaccharides composition except glucose content	802:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	2	1	theme	RPM	380:382	arg1	FR					373:374	FR	373:374	FR	373:374	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	1	theme	RPM	380:382	arg1	activity					363:370	the maximum flocculation activity	338:370	the maximum flocculation activity (FR) of RPM to kolin clay	338:396	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	4	2	theme	crude	561:565	arg1	RPMP					595:598	RPMP	595:598	RPMP	595:598	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	2	theme	crude	561:565	arg1	extract					587:593	The crude RPM polysaccharides extract	557:593	The crude RPM polysaccharides extract(RPMP)	557:599	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	0	3	theme	Effective	79:87	arg1	polysaccharides					89:103	Effective polysaccharides	79:103	Effective polysaccharides	79:103	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	2	4	from	%	452:452	arg1	system					473:478	sea water assay system	457:478	sea water assay system	457:478	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	4	from	%	452:452	arg1	system					437:442	deionized water assay system	415:442	deionized water assay system	415:442	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	4	5	theme	similar	802:808	arg1	composition					826:836	similar monosaccharides composition	802:836	similar monosaccharides composition except glucose content	802:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	1	6	theme	philippinarum	187:199	arg1	resource					165:172	A novel natural bioflocculant resource	135:172	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components	135:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	0	7	theme	synergistic	109:119	arg1	flocculation					121:132	synergistic flocculation	109:132	synergistic flocculation	109:132	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	1	8	with	resource	165:172	arg1	components					258:267	the effective flocculation components	231:267	the effective flocculation components	231:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	3	9	theme	microalgae	509:518	arg1	salina					530:535	marine microalgae Chlorella salina	502:535	marine microalgae Chlorella salina	502:535	RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%.					
29455975	4	10	theme	pure	702:705	arg1	RPMP					707:710	pure RPMP	702:710	pure RPMP	702:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	11	theme	functional	677:686	arg1	components					688:697	the functional components	673:697	the functional components of pure RPMP	673:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	11	theme	functional	677:686	arg1	heteropolysaccharides					754:774	two complex heteropolysaccharides	742:774	two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content	742:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	12	theme	polysaccharides	571:585	arg1	RPMP					595:598	RPMP	595:598	RPMP	595:598	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	12	theme	polysaccharides	571:585	arg1	extract					587:593	The crude RPM polysaccharides extract	557:593	The crude RPM polysaccharides extract(RPMP)	557:599	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	3	13	theme	%	554:554	arg1	FR					544:545	a FR	542:545	a FR of 74.1%	542:554	RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%.					
29455975	6	14	theme	original	1104:1111	arg1	significant					1127:1137	significant	1127:1137	significant	1127:1137	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	6	14	theme	original	1104:1111	arg1	proportion					1113:1122	the original proportion	1100:1122	the original proportion	1100:1122	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	4	15	theme	RPM	567:569	arg1	RPMP					595:598	RPMP	595:598	RPMP	595:598	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	15	theme	RPM	567:569	arg1	extract					587:593	The crude RPM polysaccharides extract	557:593	The crude RPM polysaccharides extract(RPMP)	557:599	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	2	16	theme	Experimental	305:316	arg1	results					318:324	Experimental results	305:324	Experimental results	305:324	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	1	17	theme	conglutination	201:214	arg1	mud					216:218	conglutination mud	201:218	Ruditapes philippinarum conglutination mud (RPM)	177:224	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	4	18	theme	RPMP-1	779:784	arg1	components					688:697	the functional components	673:697	the functional components of pure RPMP	673:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	18	theme	RPMP-1	779:784	arg1	heteropolysaccharides					754:774	two complex heteropolysaccharides	742:774	two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content	742:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	19	theme	RPMP	707:710	arg1	components					688:697	the functional components	673:697	the functional components of pure RPMP	673:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	19	theme	RPMP	707:710	arg1	heteropolysaccharides					754:774	two complex heteropolysaccharides	742:774	two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content	742:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	6	20	theme	%	1046:1046	arg1	activity					1030:1037	synergistic flocculation activity	1005:1037	synergistic flocculation activity of 65.6%	1005:1046	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	2	21	theme	maximum	342:348	arg1	FR					373:374	FR	373:374	FR	373:374	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	21	theme	maximum	342:348	arg1	activity					363:370	the maximum flocculation activity	338:370	the maximum flocculation activity (FR) of RPM to kolin clay	338:396	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	22	theme	flocculation	350:361	arg1	FR					373:374	FR	373:374	FR	373:374	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	22	theme	flocculation	350:361	arg1	activity					363:370	the maximum flocculation activity	338:370	the maximum flocculation activity (FR) of RPM to kolin clay	338:396	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	4	23	theme	RPMP-2	790:795	arg1	components					688:697	the functional components	673:697	the functional components of pure RPMP	673:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	23	theme	RPMP-2	790:795	arg1	heteropolysaccharides					754:774	two complex heteropolysaccharides	742:774	two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content	742:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	1	24	dep	philippinarum	187:199	arg1	mud					216:218	conglutination mud	201:218	Ruditapes philippinarum conglutination mud (RPM)	177:224	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	6	25	theme	flocculation	1017:1028	arg1	activity					1030:1037	synergistic flocculation activity	1005:1037	synergistic flocculation activity of 65.6%	1005:1046	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	0	26	theme	natural	18:24	arg1	resource					40:47	a novel natural bioflocculant resource	10:47	a novel natural bioflocculant resource from Ruditapes philippinarum	10:76	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	5	27	theme	RPMP-1	887:892	arg1	weights					876:882	The molecular weights	862:882	The molecular weights of RPMP-1 and RPMP-2	862:903	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	5	27	theme	RPMP-1	887:892	arg1	5.7 kDa					910:916	5.7 kDa	910:916	5.7 kDa	910:916	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	6	28	theme	crude	1077:1081	arg1	RPMP					1083:1086	crude RPMP	1077:1086	crude RPMP	1077:1086	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	2	29	theme	assay	431:435	arg1	system					437:442	deionized water assay system	415:442	deionized water assay system	415:442	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	0	30	theme	novel	12:16	arg1	resource					40:47	a novel natural bioflocculant resource	10:47	a novel natural bioflocculant resource from Ruditapes philippinarum	10:76	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	5	31	theme	RPMP-2	898:903	arg1	weights					876:882	The molecular weights	862:882	The molecular weights of RPMP-1 and RPMP-2	862:903	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	5	31	theme	RPMP-2	898:903	arg1	5.7 kDa					910:916	5.7 kDa	910:916	5.7 kDa	910:916	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	3	32	theme	marine	502:507	arg1	salina					530:535	marine microalgae Chlorella salina	502:535	marine microalgae Chlorella salina	502:535	RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%.					
29455975	2	33	theme	water	425:429	arg1	system					437:442	deionized water assay system	415:442	deionized water assay system	415:442	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	34	theme	assay	467:471	arg1	system					473:478	sea water assay system	457:478	sea water assay system	457:478	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	6	35	theme	2:1	1067:1069	arg1	ratio					1058:1062	a mass ratio	1051:1062	a mass ratio of 2:1	1051:1069	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	4	36	theme	complex	746:752	arg1	components					688:697	the functional components	673:697	the functional components of pure RPMP	673:710	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	36	theme	complex	746:752	arg1	heteropolysaccharides					754:774	two complex heteropolysaccharides	742:774	two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content	742:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	2	37	theme	deionized	415:423	arg1	water					425:429	deionized water	415:429	deionized water assay system	415:442	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	38	theme	water	461:465	arg1	system					473:478	sea water assay system	457:478	sea water assay system	457:478	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	0	39	theme	bioflocculant	26:38	arg1	resource					40:47	a novel natural bioflocculant resource	10:47	a novel natural bioflocculant resource from Ruditapes philippinarum	10:76	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	1	40	theme	effective	235:243	arg1	components					258:267	the effective flocculation components	231:267	the effective flocculation components	231:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	2	41	theme	sea	457:459	arg1	system					473:478	sea water assay system	457:478	sea water assay system	457:478	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	0	42	from	philippinarum	64:76	arg1	resource					40:47	a novel natural bioflocculant resource	10:47	a novel natural bioflocculant resource from Ruditapes philippinarum	10:76	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	6	43	theme	mass	1053:1056	arg1	ratio					1058:1062	a mass ratio	1051:1062	a mass ratio of 2:1	1051:1069	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	5	44	theme	molecular	866:874	arg1	weights					876:882	The molecular weights	862:882	The molecular weights of RPMP-1 and RPMP-2	862:903	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	5	44	theme	molecular	866:874	arg1	5.7 kDa					910:916	5.7 kDa	910:916	5.7 kDa	910:916	The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively.					
29455975	4	45	theme	glucose	845:851	arg1	content					853:859	glucose content	845:859	glucose content	845:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	2	46	from	%	410:410	arg1	system					473:478	sea water assay system	457:478	sea water assay system	457:478	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	2	46	from	%	410:410	arg1	system					437:442	deionized water assay system	415:442	deionized water assay system	415:442	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	1	47	theme	novel	137:141	arg1	resource					165:172	A novel natural bioflocculant resource	135:172	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components	135:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	4	48	dep	%	622:622	arg1	polysaccharides					630:644	polysaccharides	630:644	97.8% (w/w) polysaccharides	618:644	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	4	48	dep	%	622:622	arg1	protein					661:667	protein	661:667	protein	661:667	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	6	49	theme	synergistic	1142:1152	arg1	flocculation					1154:1165	synergistic flocculation	1142:1165	synergistic flocculation	1142:1165	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	1	50	theme	natural	143:149	arg1	resource					165:172	A novel natural bioflocculant resource	135:172	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components	135:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	1	51	theme	flocculation	245:256	arg1	components					258:267	the effective flocculation components	231:267	the effective flocculation components	231:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	1	52	theme	bioflocculant	151:163	arg1	resource					165:172	A novel natural bioflocculant resource	135:172	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components	135:267	A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study.					
29455975	4	53	with	heteropolysaccharides	754:774	arg1	composition					826:836	similar monosaccharides composition	802:836	similar monosaccharides composition except glucose content	802:859	The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content.					
29455975	6	54	theme	synergistic	1005:1015	arg1	activity					1030:1037	synergistic flocculation activity	1005:1037	synergistic flocculation activity of 65.6%	1005:1046	It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.					
29455975	2	55	theme	kolin	387:391	arg1	clay					393:396	kolin clay	387:396	kolin clay	387:396	Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system.					
29455975	0	56	dep	Revealing	0:8	arg1	polysaccharides					89:103	Effective polysaccharides	79:103	Effective polysaccharides	79:103	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	0	56	dep	Revealing	0:8	arg1	flocculation					121:132	synergistic flocculation	109:132	synergistic flocculation	109:132	Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation.					
29455975	3	57	theme	Chlorella	520:528	arg1	salina					530:535	marine microalgae Chlorella salina	502:535	marine microalgae Chlorella salina	502:535	RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%.					
30071672	0	0	theme	Anti-Allergic	81:93	arg1	Effect					95:100	Anti-Allergic Effect	81:100	Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix	81:159	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	8	1	theme	molar	1188:1192	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	6	2	theme	delayed-type	779:790	arg1	DTH					810:812	DTH	810:812	DTH	810:812	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	6	2	theme	delayed-type	779:790	arg1	hypersensitivity					792:807	delayed-type hypersensitivity	779:807	a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days	777:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	8	3	theme	relative	1179:1186	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	1	4	theme	Allergic	174:181	arg1	disease					212:218	a common clinical disease	194:218	a common clinical disease	194:218	Background: Allergic disease is a common clinical disease.					
30071672	1	4	theme	Allergic	174:181	arg1	disease					183:189	Allergic disease	174:189	Allergic disease	174:189	Background: Allergic disease is a common clinical disease.					
30071672	1	4	theme	Allergic	174:181	arg1	Background					162:171	Background	162:171	Background: Allergic disease is a common clinical disease.	162:219	Background: Allergic disease is a common clinical disease.					
30071672	4	5	dep	composition	481:491	arg1	The					477:479	The	477:479	The	477:479	The composition and content of monosaccharides were determined to provide a material basis.					
30071672	12	6	theme	natural	1661:1667	arg1	drugs					1683:1687	natural anti-allergic drugs	1661:1687	natural anti-allergic drugs	1661:1687	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	12	7	theme	potential	1638:1646	arg1	resource					1648:1655	a novel potential resource	1630:1655	a novel potential resource for natural anti-allergic drugs	1630:1687	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	12	7	theme	potential	1638:1646	arg1	SRPS					1622:1625	SRPS	1622:1625	SRPS	1622:1625	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	3	8	dep	polysaccharides	404:418	arg1	purified					434:441	purified	434:441	purified from Saposhnikoviae Radix (SRPS)	434:474	This study was designed to evaluate the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS).					
30071672	3	8	dep	polysaccharides	404:418	arg1	extracted					420:428	extracted	420:428	extracted	420:428	This study was designed to evaluate the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS).					
30071672	12	9	theme	anti-allergic	1669:1681	arg1	drugs					1683:1687	natural anti-allergic drugs	1661:1687	natural anti-allergic drugs	1661:1687	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	2	10	theme	anti-allergic	296:308	arg1	agents					310:315	potential anti-allergic agents	286:315	potential anti-allergic agents	286:315	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	6	11	dep	model	821:825	arg1	SRPS					848:851	orally administrated SRPS	827:851	a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days	777:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	9	12	theme	serum	1346:1350	arg1	levels					1356:1361	a serum IgE levels	1344:1361	a serum IgE levels	1344:1361	SRPS could effectively reduce ear swelling, a thymus index, and a serum IgE levels.					
30071672	0	13	theme	Effect	95:100	arg1	Determination					44:56	Determination	44:56	Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix	44:159	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	2	14	theme	potential	286:294	arg1	agents					310:315	potential anti-allergic agents	286:315	potential anti-allergic agents	286:315	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	7	15	theme	serum	912:916	arg1	levels					922:927	serum IgE levels	912:927	serum IgE levels	912:927	Ear swelling, organ index, and serum IgE levels were observed to evaluate the anti-allergic activities.					
30071672	9	16	theme	IgE	1352:1354	arg1	levels					1356:1361	a serum IgE levels	1344:1361	a serum IgE levels	1344:1361	SRPS could effectively reduce ear swelling, a thymus index, and a serum IgE levels.					
30071672	0	17	theme	Polysaccharides	109:123	arg1	Monosaccharides					61:75	Monosaccharides	61:75	Monosaccharides	61:75	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	0	17	theme	Polysaccharides	109:123	arg1	Effect					95:100	Anti-Allergic Effect	81:100	Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix	81:159	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	8	18	theme	%	1207:1207	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	6	19	theme	consecutive	863:873	arg1	days					875:878	seven consecutive days	857:878	seven consecutive days	857:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	7	20	theme	IgE	918:920	arg1	levels					922:927	serum IgE levels	912:927	serum IgE levels	912:927	Ear swelling, organ index, and serum IgE levels were observed to evaluate the anti-allergic activities.					
30071672	3	21	theme	anti-allergic	358:370	arg1	activities					372:381	the anti-allergic activities	354:381	the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS)	354:474	This study was designed to evaluate the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS).					
30071672	5	22	theme	chromatography-tandem	611:631	arg1	spectrometry					638:649	liquid chromatography-tandem mass spectrometry	604:649	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	5	22	theme	chromatography-tandem	611:631	arg1	UPLC-MS/MS					652:661	UPLC-MS/MS	652:661	UPLC-MS/MS	652:661	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	8	23	theme	%	1230:1230	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	0	24	theme	Sensitive	2:10	arg1	Method					33:38	A Sensitive and Rapid UPLC-MS/MS Method	0:38	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.	0:160	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	4	25	theme	monosaccharides	508:522	arg1	content					497:503	content	497:503	content	497:503	The composition and content of monosaccharides were determined to provide a material basis.					
30071672	4	25	theme	monosaccharides	508:522	arg1	composition					481:491	composition	481:491	composition	481:491	The composition and content of monosaccharides were determined to provide a material basis.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	acid					1097:1100	galacturonic acid	1084:1100	galacturonic acid	1084:1100	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	arabinose					1162:1170	arabinose	1162:1170	arabinose	1162:1170	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	mannose					1103:1109	mannose	1103:1109	mannose	1103:1109	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	rhamnose					1132:1139	rhamnose	1132:1139	rhamnose	1132:1139	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	fucose					1142:1147	fucose	1142:1147	fucose	1142:1147	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	glucose					1112:1118	glucose	1112:1118	glucose	1112:1118	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	galactose					1121:1129	galactose	1121:1129	galactose	1121:1129	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	26	theme	galacturonic	1084:1095	arg1	ribose					1150:1155	ribose	1150:1155	ribose	1150:1155	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	5	27	theme	mass	633:636	arg1	spectrometry					638:649	liquid chromatography-tandem mass spectrometry	604:649	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	5	27	theme	mass	633:636	arg1	UPLC-MS/MS					652:661	UPLC-MS/MS	652:661	UPLC-MS/MS	652:661	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	10	28	theme	monosaccharide	1485:1498	arg1	composition					1500:1510	the monosaccharide composition	1481:1510	the monosaccharide composition of SRPS	1481:1518	Conclusions: The method was simple, rapid, sensitive, and reproducible, which could be used to analyze and determine the monosaccharide composition of SRPS.					
30071672	8	29	theme	%	1237:1237	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	10	30	used	used	1451:1454	arg2	which					1436:1440	which	1436:1440	which	1436:1440	Conclusions: The method was simple, rapid, sensitive, and reproducible, which could be used to analyze and determine the monosaccharide composition of SRPS.					
30071672	5	31	theme	spectrometry	638:649	arg1	method					664:669	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	7	32	theme	organ	895:899	arg1	index					901:905	organ index	895:905	organ index	895:905	Ear swelling, organ index, and serum IgE levels were observed to evaluate the anti-allergic activities.					
30071672	1	33	theme	common	196:201	arg1	disease					212:218	a common clinical disease	194:218	a common clinical disease	194:218	Background: Allergic disease is a common clinical disease.					
30071672	1	33	theme	common	196:201	arg1	disease					183:189	Allergic disease	174:189	Allergic disease	174:189	Background: Allergic disease is a common clinical disease.					
30071672	1	33	theme	common	196:201	arg1	Background					162:171	Background	162:171	Background: Allergic disease is a common clinical disease.	162:219	Background: Allergic disease is a common clinical disease.					
30071672	0	34	theme	Rapid	16:20	arg1	Method					33:38	A Sensitive and Rapid UPLC-MS/MS Method	0:38	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.	0:160	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	7	35	theme	Ear	881:883	arg1	swelling					885:892	Ear swelling	881:892	Ear swelling	881:892	Ear swelling, organ index, and serum IgE levels were observed to evaluate the anti-allergic activities.					
30071672	6	36	theme	administrated	834:846	arg1	SRPS					848:851	orally administrated SRPS	827:851	a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days	777:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	8	37	with	galactose	1121:1129	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	1	38	theme	clinical	203:210	arg1	disease					212:218	a common clinical disease	194:218	a common clinical disease	194:218	Background: Allergic disease is a common clinical disease.					
30071672	1	38	theme	clinical	203:210	arg1	disease					183:189	Allergic disease	174:189	Allergic disease	174:189	Background: Allergic disease is a common clinical disease.					
30071672	1	38	theme	clinical	203:210	arg1	Background					162:171	Background	162:171	Background: Allergic disease is a common clinical disease.	162:219	Background: Allergic disease is a common clinical disease.					
30071672	3	39	theme	water-soluble	390:402	arg1	polysaccharides					404:418	the water-soluble polysaccharides	386:418	the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS)	386:474	This study was designed to evaluate the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS).					
30071672	6	40	theme	mouse	815:819	arg1	model					821:825	a delayed-type hypersensitivity (DTH) mouse model	777:825	a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days	777:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	11	41	theme	DTH	1617:1619	arg1	development					1589:1599	development	1589:1599	development of DNFB-induced DTH	1589:1619	The vivo experiments demonstrated that SRPS may effectively inhibit development of DNFB-induced DTH.					
30071672	2	42	theme	agents	310:315	arg1	range					277:281	a wide range	270:281	a wide range of potential anti-allergic agents	270:315	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	8	43	theme	%	1222:1222	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	44	with	mannose	1103:1109	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	2	45	theme	important	249:257	arg1	source					259:264	an important source	246:264	an important source for a wide range of potential anti-allergic agents	246:315	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	8	46	with	glucose	1112:1118	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	0	47	theme	UPLC-MS/MS	22:31	arg1	Method					33:38	A Sensitive and Rapid UPLC-MS/MS Method	0:38	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.	0:160	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	9	48	theme	a	1344:1344	arg1	levels					1356:1361	a serum IgE levels	1344:1361	a serum IgE levels	1344:1361	SRPS could effectively reduce ear swelling, a thymus index, and a serum IgE levels.					
30071672	5	49	dep	composition	704:714	arg1	the					700:702	the	700:702	the	700:702	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	8	50	theme	%	1244:1244	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	4	51	theme	material	553:560	arg1	basis					562:566	a material basis	551:566	a material basis	551:566	The composition and content of monosaccharides were determined to provide a material basis.					
30071672	5	52	theme	SRPS	731:734	arg1	content					720:726	content	720:726	content	720:726	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	5	52	theme	SRPS	731:734	arg1	composition					704:714	composition	704:714	composition	704:714	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	11	53	theme	DNFB-induced	1604:1615	arg1	DTH					1617:1619	DNFB-induced DTH	1604:1619	DNFB-induced DTH	1604:1619	The vivo experiments demonstrated that SRPS may effectively inhibit development of DNFB-induced DTH.					
30071672	8	54	theme	%	1251:1251	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	10	55	theme	SRPS	1515:1518	arg1	composition					1500:1510	the monosaccharide composition	1481:1510	the monosaccharide composition of SRPS	1481:1518	Conclusions: The method was simple, rapid, sensitive, and reproducible, which could be used to analyze and determine the monosaccharide composition of SRPS.					
30071672	2	56	theme	Natural	221:227	arg1	products					229:236	Natural products	221:236	Natural products	221:236	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	5	57	theme	ultra-high-performance	581:602	arg1	method					664:669	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	7	58	theme	anti-allergic	959:971	arg1	activities					973:982	the anti-allergic activities	955:982	the anti-allergic activities	955:982	Ear swelling, organ index, and serum IgE levels were observed to evaluate the anti-allergic activities.					
30071672	11	59	theme	vivo	1525:1528	arg1	experiments					1530:1540	The vivo experiments	1521:1540	The vivo experiments	1521:1540	The vivo experiments demonstrated that SRPS may effectively inhibit development of DNFB-induced DTH.					
30071672	2	60	theme	wide	272:275	arg1	range					277:281	a wide range	270:281	a wide range of potential anti-allergic agents	270:315	Natural products provide an important source for a wide range of potential anti-allergic agents.					
30071672	5	61	theme	liquid	604:609	arg1	spectrometry					638:649	liquid chromatography-tandem mass spectrometry	604:649	An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method	578:669	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	5	61	theme	liquid	604:609	arg1	UPLC-MS/MS					652:661	UPLC-MS/MS	652:661	UPLC-MS/MS	652:661	Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the composition and content of SRPS.					
30071672	8	62	with	fucose	1142:1147	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	12	63	theme	novel	1632:1636	arg1	resource					1648:1655	a novel potential resource	1630:1655	a novel potential resource for natural anti-allergic drugs	1630:1687	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	12	63	theme	novel	1632:1636	arg1	SRPS					1622:1625	SRPS	1622:1625	SRPS	1622:1625	SRPS is a novel potential resource for natural anti-allergic drugs.					
30071672	9	64	theme	thymus	1326:1331	arg1	index					1333:1337	a thymus index	1324:1337	a thymus index	1324:1337	SRPS could effectively reduce ear swelling, a thymus index, and a serum IgE levels.					
30071672	0	65	theme	Monosaccharides	61:75	arg1	Determination					44:56	Determination	44:56	Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix	44:159	A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix.					
30071672	8	66	with	ribose	1150:1155	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	67	theme	UPLC-MS/MS	998:1007	arg1	analysis					1009:1016	The UPLC-MS/MS analysis	994:1016	The UPLC-MS/MS analysis	994:1016	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	68	theme	%	1263:1263	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	69	with	rhamnose	1132:1139	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	8	70	theme	%	1214:1214	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	6	71	theme	hypersensitivity	792:807	arg1	model					821:825	a delayed-type hypersensitivity (DTH) mouse model	777:825	a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days	777:878	2,4-dinitrofluorobenzene (DNFB) induced a delayed-type hypersensitivity (DTH) mouse model orally administrated SRPS for seven consecutive days.					
30071672	3	72	theme	polysaccharides	404:418	arg1	activities					372:381	the anti-allergic activities	354:381	the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS)	354:474	This study was designed to evaluate the anti-allergic activities of the water-soluble polysaccharides extracted and purified from Saposhnikoviae Radix (SRPS).					
30071672	8	73	with	arabinose	1162:1170	arg1	ratio					1194:1198	a relative molar ratio	1177:1198	a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively	1177:1277	Results: The UPLC-MS/MS analysis showed that SRPS was consisted of eight monosaccharides including galacturonic acid, mannose, glucose, galactose, rhamnose, fucose, ribose, and arabinose with a relative molar ratio of 4.42%, 7.86%, 23.69%, 12.06%, 3.10%, 0.45%, 0.71%, and 47.70%, respectively.					
30071672	9	74	theme	ear	1310:1312	arg1	swelling					1314:1321	ear swelling	1310:1321	ear swelling	1310:1321	SRPS could effectively reduce ear swelling, a thymus index, and a serum IgE levels.					
29600528	2	0	theme	inimer-type	543:553	arg1	agent					575:579	an inimer-type RAFT chain transfer agent	540:579	an inimer-type RAFT chain transfer agent	540:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	1	1	theme	hyperbranched	180:192	arg1	glycopolymers					194:206	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	0	2	theme	Hepatoma-Targeted	93:109	arg1	Imaging					111:117	Hepatoma-Targeted Imaging	93:117	Hepatoma-Targeted Imaging	93:117	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	5	3	theme	bioimaging	1194:1203	arg1	fields					1205:1210	the bioimaging fields	1190:1210	the bioimaging fields	1190:1210	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	5	4	theme	potential	1165:1173	arg1	application					1175:1185	their potential application	1159:1185	their potential application in the bioimaging fields	1159:1210	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	5	5	theme	hyperbranched	998:1010	arg1	polymers					1012:1019	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	6	theme	addition-fragmentation	371:392	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	3	7	theme	good	769:772	arg1	photostability					774:787	good photostability	769:787	good photostability	769:787	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	5	8	theme	fluorescent	986:996	arg1	polymers					1012:1019	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	4	9	theme	excellent	829:837	arg1	biocompatibility					839:854	the excellent biocompatibility	825:854	the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells	825:931	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	1	10	theme	facile	287:292	arg1	strategy					306:313	a facile "bottom-up" strategy	285:313	a facile "bottom-up" strategy	285:313	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	3	11	theme	resulting	607:615	arg1	hPGVB					629:633	the resulting amphiphilic hPGVB	603:633	the resulting amphiphilic hPGVB	603:633	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	2	12	theme	chain	394:398	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	4	13	theme	viability	799:807	arg1	study					809:813	The cell viability study	790:813	The cell viability study	790:813	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	2	14	theme	agent	575:579	arg1	presence					528:535	presence	528:535	presence of an inimer-type RAFT chain transfer agent	528:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	4	15	theme	cell	794:797	arg1	viability					799:807	The cell viability	790:807	The cell viability study	790:813	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	4	16	theme	fluorescent	869:879	arg1	FNPs					896:899	FNPs	896:899	FNPs	896:899	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	4	16	theme	fluorescent	869:879	arg1	nanoparticles					881:893	the hPGVB fluorescent nanoparticles	859:893	the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells	859:931	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	2	17	theme	transfer	566:573	arg1	agent					575:579	an inimer-type RAFT chain transfer agent	540:579	an inimer-type RAFT chain transfer agent	540:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	18	theme	monomers	511:518	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	18	theme	monomers	511:518	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	5	19	theme	ASGP	1115:1118	arg1	cells					1141:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	20	theme	fluorescent	499:509	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	20	theme	fluorescent	499:509	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	1	21	theme	bottom-up	295:303	arg1	strategy					306:313	a facile "bottom-up" strategy	285:313	a facile "bottom-up" strategy	285:313	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	1	22	dep	glycopolymers	194:206	arg1	GalEA-co-VBPT-co-BYMA					212:232	GalEA-co-VBPT-co-BYMA	212:232	GalEA-co-VBPT-co-BYMA	212:232	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	1	22	dep	glycopolymers	194:206	arg1	hPGVB					236:240	hPGVB	236:240	hPGVB	236:240	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	1	22	dep	glycopolymers	194:206	arg1	h-P					208:210	h-P	208:210	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	2	23	theme	RAFT	410:413	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	24	theme	methacrylate-type	481:497	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	24	theme	methacrylate-type	481:497	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	1	25	theme	"	304:304	arg1	strategy					306:313	a facile "bottom-up" strategy	285:313	a facile "bottom-up" strategy	285:313	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	3	26	theme	stable	658:663	arg1	nanoparticles					665:677	stable nanoparticles	658:677	stable nanoparticles	658:677	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	1	27	theme	novel	122:126	arg1	type					128:131	A novel type	120:131	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	120:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	5	28	theme	receptor-rich	1121:1133	arg1	cells					1141:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	0	29	theme	"	10:10	arg1	Fabrication					12:22	"Bottom-Up" Fabrication	0:22	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.	0:118	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	5	30	theme	hPEVB	1049:1053	arg1	FNPs					1055:1058	hPEVB FNPs	1049:1058	hPEVB FNPs	1049:1058	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	1	31	theme	glycopolymers	194:206	arg1	type					128:131	A novel type	120:131	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	120:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	5	32	from	application	1175:1185	arg1	fields					1205:1210	the bioimaging fields	1190:1210	the bioimaging fields	1190:1210	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	33	theme	monomers	460:467	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	0	34	theme	BODIPY-Functionalized	27:47	arg1	Glycopolymers					75:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	2	35	theme	one-pot	352:358	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	4	36	theme	HepG2	910:914	arg1	cells					927:931	HepG2 and NIH3T3 cells	910:931	cells	927:931	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	2	37	theme	galactose	450:458	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	37	theme	galactose	450:458	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	37	theme	galactose	450:458	arg1	AcGalEA					469:475	AcGalEA	469:475	AcGalEA	469:475	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	5	38	gly	asialoglycoprotein	1095:1112	arg1	asialoglycoprotein					1095:1112	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	3	39	theme	strong	705:710	arg1	fluorescence					718:729	strong green fluorescence	705:729	strong green fluorescence	705:729	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	5	40	theme	HepG2	1135:1139	arg1	cells					1141:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	41	theme	acrylate-type	436:448	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	41	theme	acrylate-type	436:448	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	41	theme	acrylate-type	436:448	arg1	AcGalEA					469:475	AcGalEA	469:475	AcGalEA	469:475	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	0	42	theme	Hyperbranched	61:73	arg1	Glycopolymers					75:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	5	43	theme	galactose-free	971:984	arg1	polymers					1012:1019	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	44	theme	RAFT	555:558	arg1	agent					575:579	an inimer-type RAFT chain transfer agent	540:579	an inimer-type RAFT chain transfer agent	540:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	4	45	theme	NIH3T3	920:925	arg1	cells					927:931	HepG2 and NIH3T3 cells	910:931	cells	927:931	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	5	46	dep	polymers	1012:1019	arg1	OEGMA-co-VBPT-co-BYMA					1025:1045	OEGMA-co-VBPT-co-BYMA	1025:1045	OEGMA-co-VBPT-co-BYMA	1025:1045	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	5	46	dep	polymers	1012:1019	arg1	h-P					1021:1023	h-P	1021:1023	the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA)	967:1046	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	47	theme	reversible	360:369	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	0	48	theme	Fluorescent	49:59	arg1	Glycopolymers					75:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers	27:87	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	3	49	theme	amphiphilic	617:627	arg1	hPGVB					629:633	the resulting amphiphilic hPGVB	603:633	the resulting amphiphilic hPGVB	603:633	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	3	50	theme	quantum	750:756	arg1	yields					758:763	relative high quantum yields	736:763	relative high quantum yields	736:763	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	2	51	dep	monomers	460:467	arg1	monomers					460:467	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA	436:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	51	dep	monomers	460:467	arg1	BYMA					520:523	methacrylate-type fluorescent monomers BYMA	481:523	methacrylate-type fluorescent monomers BYMA	481:523	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	2	51	dep	monomers	460:467	arg1	AcGalEA					469:475	AcGalEA	469:475	AcGalEA	469:475	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	1	52	theme	multivalent	136:146	arg1	glycopolymers					194:206	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	5	53	theme	asialoglycoprotein	1095:1112	arg1	cells					1141:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	asialoglycoprotein (ASGP) receptor-rich HepG2 cells	1095:1145	More importantly, comparing with the galactose-free fluorescent hyperbranched polymers h-P(OEGMA-co-VBPT-co-BYMA), hPEVB FNPs can be selectively internalized by asialoglycoprotein (ASGP) receptor-rich HepG2 cells, indicating their potential application in the bioimaging fields.					
29600528	2	54	from	copolymerization	416:431	arg1	presence					528:535	presence	528:535	presence of an inimer-type RAFT chain transfer agent	528:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	3	55	theme	green	712:716	arg1	fluorescence					718:729	strong green fluorescence	705:729	strong green fluorescence	705:729	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	2	56	theme	acetylated	320:329	arg1	hPGVB					331:335	The acetylated hPGVB	316:335	The acetylated hPGVB	316:335	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	3	57	theme	relative	736:743	arg1	yields					758:763	relative high quantum yields	736:763	relative high quantum yields	736:763	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	4	58	theme	nanoparticles	881:893	arg1	biocompatibility					839:854	the excellent biocompatibility	825:854	the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells	825:931	The cell viability study indicates the excellent biocompatibility of the hPGVB fluorescent nanoparticles (FNPs) against HepG2 and NIH3T3 cells.					
29600528	1	59	theme	fluorescent	168:178	arg1	glycopolymers					194:206	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	2	60	theme	chain	560:564	arg1	agent					575:579	an inimer-type RAFT chain transfer agent	540:579	an inimer-type RAFT chain transfer agent	540:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
29600528	3	61	theme	high	745:748	arg1	yields					758:763	relative high quantum yields	736:763	relative high quantum yields	736:763	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	3	62	theme	aqueous	682:688	arg1	media					690:694	aqueous media	682:694	aqueous media	682:694	After deacetylation, the resulting amphiphilic hPGVB can self-assemble into stable nanoparticles in aqueous media, showing strong green fluorescence with relative high quantum yields and good photostability.					
29600528	1	63	theme	specific	159:166	arg1	glycopolymers					194:206	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB)	136:241	A novel type of multivalent and highly specific fluorescent hyperbranched glycopolymers h-P(GalEA-co-VBPT-co-BYMA) (hPGVB) is designed and prepared successfully via a facile "bottom-up" strategy.					
29600528	0	64	theme	Glycopolymers	75:87	arg1	Fabrication					12:22	"Bottom-Up" Fabrication	0:22	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.	0:118	"Bottom-Up" Fabrication of BODIPY-Functionalized Fluorescent Hyperbranched Glycopolymers for Hepatoma-Targeted Imaging.					
29600528	2	65	theme	transfer	400:407	arg1	copolymerization					416:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization	352:431	one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent	352:579	The acetylated hPGVB is prepared by one-pot reversible addition-fragmentation chain transfer (RAFT) copolymerization of acrylate-type galactose monomers AcGalEA and methacrylate-type fluorescent monomers BYMA in presence of an inimer-type RAFT chain transfer agent.					
31279876	9	0	theme	antibacterial	1438:1450	arg1	efficacy					1452:1459	significant antibacterial efficacy	1426:1459	significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1426:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	1	1	theme	positive	153:160	arg1	impacts					162:168	the positive impacts	149:168	the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition	149:248	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	2	2	from	work	282:285	arg1	effect					294:299	the effect	290:299	the effect of the aforementioned bio-glass on the polymer matrix composites	290:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	0	3	theme	tissue	75:80	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	3	4	theme	bone	487:490	arg1	scaffolds					502:510	the bone composite scaffolds	483:510	the bone composite scaffolds	483:510	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	2	5	from	effect	294:299	arg1	composites					355:364	the polymer matrix composites	336:364	the polymer matrix composites	336:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	9	6	theme	incubation	1287:1296	arg1	60 days					1276:1282	60 days	1276:1282	60 days of incubation in PBS solution	1276:1312	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	5	7	theme	scaffolds	735:743	arg1	strength					690:697	Compression strength	678:697	Compression strength of obtained highly porous composite scaffolds	678:743	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	1	8	from	impacts	162:168	arg1	composition					238:248	the bioactive glasses composition	216:248	the bioactive glasses composition	216:248	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	2	9	theme	dedicated	272:280	arg1	work					282:285	no dedicated work	269:285	no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites	269:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	9	10	theme	scaffolds	1486:1494	arg1	enhancement					1461:1471	significant antibacterial efficacy enhancement	1426:1471	significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1426:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	8	11	theme	MG-63	1216:1220	arg1	cells					1222:1226	MG-63 cells	1216:1226	MG-63 cells	1216:1226	Cytotoxicity, cell attachment and proliferation and osteogenic differentiation were also evaluated by culturing MG-63 cells on scaffolds.					
31279876	7	12	theme	In	913:914	arg1	evaluation					922:931	In vitro evaluation	913:931	In vitro evaluation such as swelling, bio-mineralization, biodegradation	913:984	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	6	13	theme	compressive	821:831	arg1	1.7 MPa					843:849	1.7 MPa	843:849	1.7 MPa	843:849	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	6	13	theme	compressive	821:831	arg1	strength					833:840	The maximum compressive strength	809:840	The maximum compressive strength (1.7 MPa)	809:850	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	9	14	from	60 days	1276:1282	arg1	solution					1305:1312	PBS solution	1301:1312	PBS solution	1301:1312	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	3	15	theme	mechanical	458:467	arg1	properties					469:478	mechanical properties	458:478	mechanical properties	458:478	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	9	16	from	incubation	1287:1296	arg1	solution					1305:1312	PBS solution	1301:1312	PBS solution	1301:1312	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	1	17	theme	bone	114:117	arg1	field					132:136	bone regeneration field	114:136	bone regeneration field	114:136	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	6	18	theme	maximum	813:819	arg1	1.7 MPa					843:849	1.7 MPa	843:849	1.7 MPa	843:849	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	6	18	theme	maximum	813:819	arg1	strength					833:840	The maximum compressive strength	809:840	The maximum compressive strength (1.7 MPa)	809:850	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	5	19	theme	particles	798:806	arg1	presence					776:783	the presence	772:783	the presence of bio-glass particles	772:806	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	5	20	theme	bio-glass	788:796	arg1	particles					798:806	bio-glass particles	788:806	bio-glass particles	788:806	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	3	21	theme	key	371:373	arg1	idea					375:378	The key idea	367:378	The key idea of the approach	367:394	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	9	22	theme	PBS	1301:1303	arg1	solution					1305:1312	PBS solution	1301:1312	PBS solution	1301:1312	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	3	23	theme	antibacterial	410:422	arg1	efficacy					424:431	antibacterial efficacy	410:431	antibacterial efficacy	410:431	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	6	24	theme	1 g	899:901	arg1	Mg-Zn-BG					903:910	alginate composite containing 1 g Mg-Zn-BG	869:910	alginate composite containing 1 g Mg-Zn-BG	869:910	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	9	25	from	solution	1305:1312	arg1	60 days					1276:1282	60 days	1276:1282	60 days of incubation in PBS solution	1276:1312	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	9	26	theme	composite	1358:1366	arg1	scaffolds					1368:1376	the composite scaffolds	1354:1376	the composite scaffolds	1354:1376	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	7	27	theme	composite	1083:1091	arg1	scaffolds					1093:1101	composite scaffolds	1083:1101	composite scaffolds	1083:1101	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	1	28	theme	presence	177:184	arg1	impacts					162:168	the positive impacts	149:168	the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition	149:248	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	0	29	theme	Alginate-bioactive	0:17	arg1	glass					19:23	Alginate-bioactive glass	0:23	Alginate-bioactive glass	0:23	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	9	30	theme	ion	1328:1330	arg1	capability					1340:1349	the ion release capability	1324:1349	the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1324:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	1	31	theme	Past	95:98	arg1	researches					100:109	Past researches	95:109	Past researches on bone regeneration field	95:136	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	4	32	theme	XRD	664:666	arg1	analysis					668:675	SEM, ATR-FTIR and XRD analysis	646:675	SEM, ATR-FTIR and XRD analysis	646:675	The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis.					
31279876	0	33	theme	Zn	36:37	arg1	scaffolds					56:64	Zn and Mg composite scaffolds	36:64	Zn and Mg composite scaffolds for bone tissue engineering	36:92	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	3	34	contain	containing	547:556	arg2	Magnesium					567:575	Magnesium	567:575	Magnesium	567:575	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	3	34	contain	containing	547:556	arg2	Zinc					558:561	Zinc	558:561	Zinc	558:561	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	3	34	contain	containing	547:556	arg1	glasses					539:545	bioactive glasses	529:545	bioactive glasses containing Zinc and Magnesium	529:575	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	5	35	theme	obtained	702:709	arg1	scaffolds					735:743	obtained highly porous composite scaffolds	702:743	obtained highly porous composite scaffolds	702:743	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	7	36	dep	In	913:914	arg1	vitro					916:920	vitro	916:920	vitro	916:920	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	1	37	theme	Zinc	189:192	arg1	ions					208:211	Zinc and Magnesium ions	189:211	ions	208:211	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	9	38	theme	Mg	1399:1400	arg1	ions					1402:1405	Mg ions	1399:1405	Mg ions	1399:1405	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	8	39	theme	cell	1118:1121	arg1	attachment					1123:1132	cell attachment	1118:1132	cell attachment	1118:1132	Cytotoxicity, cell attachment and proliferation and osteogenic differentiation were also evaluated by culturing MG-63 cells on scaffolds.					
31279876	6	40	theme	containing	888:897	arg1	Mg-Zn-BG					903:910	alginate composite containing 1 g Mg-Zn-BG	869:910	alginate composite containing 1 g Mg-Zn-BG	869:910	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	3	41	theme	composite	492:500	arg1	scaffolds					502:510	the bone composite scaffolds	483:510	the bone composite scaffolds	483:510	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	0	42	theme	Mg	43:44	arg1	scaffolds					56:64	Zn and Mg composite scaffolds	36:64	Zn and Mg composite scaffolds for bone tissue engineering	36:92	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	2	43	theme	aforementioned	308:321	arg1	bio-glass					323:331	the aforementioned bio-glass	304:331	the aforementioned bio-glass	304:331	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	5	44	theme	porous	718:723	arg1	scaffolds					735:743	obtained highly porous composite scaffolds	702:743	obtained highly porous composite scaffolds	702:743	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	6	45	theme	composite	878:886	arg1	Mg-Zn-BG					903:910	alginate composite containing 1 g Mg-Zn-BG	869:910	alginate composite containing 1 g Mg-Zn-BG	869:910	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	1	46	theme	Magnesium	198:206	arg1	ions					208:211	Zinc and Magnesium ions	189:211	ions	208:211	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	3	47	theme	approach	387:394	arg1	idea					375:378	The key idea	367:378	The key idea of the approach	367:394	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	5	48	theme	composite	725:733	arg1	scaffolds					735:743	obtained highly porous composite scaffolds	702:743	obtained highly porous composite scaffolds	702:743	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	6	49	theme	alginate	869:876	arg1	Mg-Zn-BG					903:910	alginate composite containing 1 g Mg-Zn-BG	869:910	alginate composite containing 1 g Mg-Zn-BG	869:910	The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG.					
31279876	9	50	theme	efficacy	1452:1459	arg1	enhancement					1461:1471	significant antibacterial efficacy enhancement	1426:1471	significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1426:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	7	51	theme	bio-glass	1042:1050	arg1	incorporation					1025:1037	incorporation	1025:1037	incorporation of bio-glass	1025:1050	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	1	52	theme	ions	208:211	arg1	presence					177:184	the presence	173:184	the presence of Zinc and Magnesium ions	173:211	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	8	53	theme	osteogenic	1156:1165	arg1	differentiation					1167:1181	osteogenic differentiation	1156:1181	osteogenic differentiation	1156:1181	Cytotoxicity, cell attachment and proliferation and osteogenic differentiation were also evaluated by culturing MG-63 cells on scaffolds.					
31279876	2	54	theme	bio-glass	323:331	arg1	effect					294:299	the effect	290:299	the effect of the aforementioned bio-glass on the polymer matrix composites	290:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	0	55	theme	composite	46:54	arg1	scaffolds					56:64	Zn and Mg composite scaffolds	36:64	Zn and Mg composite scaffolds for bone tissue engineering	36:92	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	5	56	theme	Compression	678:688	arg1	strength					690:697	Compression strength	678:697	Compression strength of obtained highly porous composite scaffolds	678:743	Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles.					
31279876	3	57	theme	alginate	582:589	arg1	networks					591:598	alginate networks	582:598	alginate networks	582:598	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	1	58	theme	regeneration	119:130	arg1	field					132:136	bone regeneration field	114:136	bone regeneration field	114:136	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	1	59	from	researches	100:109	arg1	field					132:136	bone regeneration field	114:136	bone regeneration field	114:136	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	9	60	dep	scaffolds	1368:1376	arg1	Zn					1392:1393	Zn	1392:1393	Zn	1392:1393	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	9	60	dep	scaffolds	1368:1376	arg1	ions					1402:1405	Mg ions	1399:1405	Mg ions	1399:1405	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	7	61	theme	apatite	1061:1067	arg1	deposition					1069:1078	apatite deposition	1061:1078	apatite deposition on composite scaffolds	1061:1101	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	9	62	dep	coli	1507:1510	arg1	bacteria					1526:1533	bacteria	1526:1533	bacteria	1526:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	9	63	theme	ICP	1242:1244	arg1	analysis					1246:1253	ICP analysis	1242:1253	ICP analysis	1242:1253	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	9	64	theme	scaffolds	1368:1376	arg1	capability					1340:1349	the ion release capability	1324:1349	the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1324:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	9	65	theme	significant	1426:1436	arg1	efficacy					1452:1459	significant antibacterial efficacy	1426:1459	significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1426:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	4	66	theme	prepared	605:612	arg1	scaffolds					614:622	The prepared scaffolds	601:622	The prepared scaffolds	601:622	The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis.					
31279876	9	67	theme	composite	1476:1484	arg1	scaffolds					1486:1494	composite scaffolds	1476:1494	composite scaffolds against E. coli and S. aureus bacteria	1476:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	3	68	theme	bioactive	529:537	arg1	glasses					539:545	bioactive glasses	529:545	bioactive glasses containing Zinc and Magnesium	529:575	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	3	69	theme	scaffolds	502:510	arg1	properties					469:478	mechanical properties	458:478	mechanical properties	458:478	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	3	69	theme	scaffolds	502:510	arg1	efficacy					424:431	antibacterial efficacy	410:431	antibacterial efficacy	410:431	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	3	69	theme	scaffolds	502:510	arg1	activity					445:452	biological activity	434:452	biological activity	434:452	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	7	70	from	deposition	1069:1078	arg1	scaffolds					1093:1101	composite scaffolds	1083:1101	composite scaffolds	1083:1101	In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds.					
31279876	0	71	theme	bone	70:73	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.					
31279876	4	72	theme	SEM	646:648	arg1	analysis					668:675	SEM, ATR-FTIR and XRD analysis	646:675	SEM, ATR-FTIR and XRD analysis	646:675	The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis.					
31279876	3	73	theme	biological	434:443	arg1	activity					445:452	biological activity	434:452	biological activity	434:452	The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks.					
31279876	2	74	theme	matrix	348:353	arg1	composites					355:364	the polymer matrix composites	336:364	the polymer matrix composites	336:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	4	75	theme	ATR-FTIR	651:658	arg1	analysis					668:675	SEM, ATR-FTIR and XRD analysis	646:675	SEM, ATR-FTIR and XRD analysis	646:675	The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis.					
31279876	1	76	theme	bioactive	220:228	arg1	composition					238:248	the bioactive glasses composition	216:248	the bioactive glasses composition	216:248	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31279876	9	77	theme	release	1332:1338	arg1	capability					1340:1349	the ion release capability	1324:1349	the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria	1324:1533	ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.					
31279876	2	78	theme	polymer	340:346	arg1	composites					355:364	the polymer matrix composites	336:364	the polymer matrix composites	336:364	However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites.					
31279876	1	79	theme	glasses	230:236	arg1	composition					238:248	the bioactive glasses composition	216:248	the bioactive glasses composition	216:248	Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition.					
31220498	5	0	theme	SVP2-2	1097:1102	arg1	capability					1083:1092	the stronger anticoagulant capability	1056:1092	the stronger anticoagulant capability of SVP2-2	1056:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	1	theme	analysis	872:879	arg1	comparison					837:846	Further comparison	829:846	Further comparison of their monosaccharide analysis and NMR spectra	829:895	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	3	2	theme	1 → with	697:704	arg1	backbone					644:651	its backbone	640:651	its backbone	640:651	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	2	theme	1 → with	697:704	arg1	Xyl					706:708	→6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc	671:715	Xyl	706:708	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	3	contain	had	587:589	arg1	SVP2-2					580:585	SVP2-2	580:585	SVP2-2	580:585	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	3	contain	had	587:589	arg2	activity					614:621	stronger anticoagulant activity	591:621	stronger anticoagulant activity	591:621	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	0	4	theme	yessoensis	96:105	arg1	viscera					107:113	Patinopecten yessoensis viscera	83:113	Patinopecten yessoensis viscera	83:113	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	0	5	from	characterization	11:26	arg1	viscera					107:113	Patinopecten yessoensis viscera	83:113	Patinopecten yessoensis viscera	83:113	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	5	6	theme	sulfate	981:987	arg1	content					989:995	the higher sulfate content	970:995	the higher sulfate content	970:995	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	6	theme	sulfate	981:987	arg1	reasons					1044:1050	the possible reasons	1031:1050	the possible reasons for the stronger anticoagulant capability of SVP2-2	1031:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	6	theme	sulfate	981:987	arg1	weight					1017:1022	lower molecular weight	1001:1022	lower molecular weight	1001:1022	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	7	theme	same	937:940	arg1	features					957:964	the same core structure features	933:964	the same core structure features	933:964	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	6	8	theme	present	1109:1115	arg1	study					1117:1121	The present study	1105:1121	The present study	1105:1121	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	1	9	theme	Sepharose	273:281	arg1	CL-6B					283:287	Sepharose CL-6B	273:287	Sepharose CL-6B	273:287	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	6	10	theme	acidic	1132:1137	arg1	candidates					1203:1212	promising anticoagulant candidates	1179:1212	promising anticoagulant candidates	1179:1212	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	6	10	theme	acidic	1132:1137	arg1	polysaccharides					1139:1153	acidic polysaccharides	1132:1153	acidic polysaccharides from scallop viscera	1132:1174	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	3	11	theme	-α-Manp	674:680	arg1	backbone					644:651	its backbone	640:651	its backbone	640:651	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	11	theme	-α-Manp	674:680	arg1	Xyl					706:708	→6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc	671:715	Xyl	706:708	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	6	12	theme	scallop	1160:1166	arg1	viscera					1168:1174	scallop viscera	1160:1174	scallop viscera	1160:1174	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	5	13	theme	core	942:945	arg1	features					957:964	the same core structure features	933:964	the same core structure features	933:964	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	14	theme	stronger	1060:1067	arg1	capability					1083:1092	the stronger anticoagulant capability	1056:1092	the stronger anticoagulant capability of SVP2-2	1056:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	15	theme	structure	947:955	arg1	features					957:964	the same core structure features	933:964	the same core structure features	933:964	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	3	16	theme	-α-Galp	689:695	arg1	backbone					644:651	its backbone	640:651	its backbone	640:651	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	16	theme	-α-Galp	689:695	arg1	Xyl					706:708	→6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc	671:715	Xyl	706:708	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	6	17	from	viscera	1168:1174	arg1	candidates					1203:1212	promising anticoagulant candidates	1179:1212	promising anticoagulant candidates	1179:1212	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	6	17	from	viscera	1168:1174	arg1	polysaccharides					1139:1153	acidic polysaccharides	1132:1153	acidic polysaccharides from scallop viscera	1132:1174	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	5	18	theme	Further	829:835	arg1	comparison					837:846	Further comparison	829:846	Further comparison of their monosaccharide analysis and NMR spectra	829:895	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	0	19	from	activity	46:53	arg1	viscera					107:113	Patinopecten yessoensis viscera	83:113	Patinopecten yessoensis viscera	83:113	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	2	20	theme	activated	326:334	arg1	time					359:362	activated partial thromboplastin time	326:362	activated partial thromboplastin time (APTT)	326:369	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	2	20	theme	activated	326:334	arg1	APTT					365:368	APTT	365:368	APTT	365:368	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	0	21	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	4	22	theme	NMR	815:817	arg1	analysis					819:826	NMR analysis	815:826	NMR analysis	815:826	Based on monosaccharide composition analysis, methylation analysis, and NMR analysis.					
31220498	0	23	theme	anticoagulant	32:44	arg1	activity					46:53	anticoagulant activity	32:53	anticoagulant activity	32:53	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	6	24	theme	promising	1179:1187	arg1	candidates					1203:1212	promising anticoagulant candidates	1179:1212	promising anticoagulant candidates	1179:1212	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	6	24	theme	promising	1179:1187	arg1	polysaccharides					1139:1153	acidic polysaccharides	1132:1153	acidic polysaccharides from scallop viscera	1132:1174	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	5	25	theme	lower	1001:1005	arg1	content					989:995	the higher sulfate content	970:995	the higher sulfate content	970:995	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	25	theme	lower	1001:1005	arg1	reasons					1044:1050	the possible reasons	1031:1050	the possible reasons for the stronger anticoagulant capability of SVP2-2	1031:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	25	theme	lower	1001:1005	arg1	weight					1017:1022	lower molecular weight	1001:1022	lower molecular weight	1001:1022	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	26	contain	possess	925:931	arg2	features					957:964	the same core structure features	933:964	the same core structure features	933:964	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	26	contain	possess	925:931	arg1	SVP2-2					918:923	SVP2-2	918:923	SVP2-2	918:923	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	26	contain	possess	925:931	arg1	SVP2-1					907:912	SVP2-1	907:912	SVP2-1	907:912	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	1	27	theme	Patinopecten	197:208	arg1	viscera					221:227	Patinopecten yessoensis viscera	197:227	Patinopecten yessoensis viscera	197:227	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	2	28	theme	common	554:559	arg1	pathways					561:568	intrinsic and common pathways	540:568	intrinsic and common pathways	540:568	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	0	29	from	viscera	107:113	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	0	29	from	viscera	107:113	arg1	activity					46:53	anticoagulant activity	32:53	anticoagulant activity	32:53	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	0	29	from	viscera	107:113	arg1	polysaccharides					62:76	two polysaccharides	58:76	two polysaccharides from Patinopecten yessoensis viscera	58:113	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	1	30	theme	present	123:129	arg1	study					131:135	the present study	119:135	the present study	119:135	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	1	31	theme	yessoensis	210:219	arg1	viscera					221:227	Patinopecten yessoensis viscera	197:227	Patinopecten yessoensis viscera	197:227	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	5	32	theme	possible	1035:1042	arg1	content					989:995	the higher sulfate content	970:995	the higher sulfate content	970:995	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	32	theme	possible	1035:1042	arg1	reasons					1044:1050	the possible reasons	1031:1050	the possible reasons for the stronger anticoagulant capability of SVP2-2	1031:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	32	theme	possible	1035:1042	arg1	weight					1017:1022	lower molecular weight	1001:1022	lower molecular weight	1001:1022	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	3	33	theme	stronger	591:598	arg1	activity					614:621	stronger anticoagulant activity	591:621	stronger anticoagulant activity	591:621	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	34	theme	Gal	738:740	arg1	C4					732:733	C4	732:733	C4 of Gal	732:740	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	2	35	theme	intrinsic	540:548	arg1	pathways					561:568	intrinsic and common pathways	540:568	intrinsic and common pathways	540:568	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	2	36	theme	fibrinogen	428:437	arg1	transformation					410:423	the transformation	406:423	the transformation of fibrinogen into fibrin (FIB)	406:455	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	0	37	theme	polysaccharides	62:76	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	0	37	theme	polysaccharides	62:76	arg1	activity					46:53	anticoagulant activity	32:53	anticoagulant activity	32:53	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	1	38	attach	isolated	183:190	arg2	SVP2-2					170:175	SVP2-2	170:175	SVP2-2	170:175	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	1	38	attach	isolated	183:190	arg1	viscera					221:227	Patinopecten yessoensis viscera	197:227	Patinopecten yessoensis viscera	197:227	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	1	38	attach	isolated	183:190	arg2	SVP2-1					159:164	SVP2-1	159:164	SVP2-1	159:164	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	1	38	attach	isolated	183:190	arg2	polysaccharides					142:156	two polysaccharides	138:156	two polysaccharides	138:156	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31220498	5	39	theme	anticoagulant	1069:1081	arg1	capability					1083:1092	the stronger anticoagulant capability	1056:1092	the stronger anticoagulant capability of SVP2-2	1056:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	40	theme	NMR	885:887	arg1	spectra					889:895	NMR spectra	885:895	NMR spectra	885:895	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	41	theme	molecular	1007:1015	arg1	content					989:995	the higher sulfate content	970:995	the higher sulfate content	970:995	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	41	theme	molecular	1007:1015	arg1	reasons					1044:1050	the possible reasons	1031:1050	the possible reasons for the stronger anticoagulant capability of SVP2-2	1031:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	41	theme	molecular	1007:1015	arg1	weight					1017:1022	lower molecular weight	1001:1022	lower molecular weight	1001:1022	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	4	42	theme	methylation	789:799	arg1	analysis					801:808	methylation analysis	789:808	methylation analysis	789:808	Based on monosaccharide composition analysis, methylation analysis, and NMR analysis.					
31220498	5	43	theme	spectra	889:895	arg1	comparison					837:846	Further comparison	829:846	Further comparison of their monosaccharide analysis and NMR spectra	829:895	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	44	theme	higher	974:979	arg1	content					989:995	the higher sulfate content	970:995	the higher sulfate content	970:995	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	44	theme	higher	974:979	arg1	reasons					1044:1050	the possible reasons	1031:1050	the possible reasons for the stronger anticoagulant capability of SVP2-2	1031:1102	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	5	44	theme	higher	974:979	arg1	weight					1017:1022	lower molecular weight	1001:1022	lower molecular weight	1001:1022	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	3	45	theme	anticoagulant	600:612	arg1	activity					614:621	stronger anticoagulant activity	591:621	stronger anticoagulant activity	591:621	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	2	46	theme	thromboplastin	344:357	arg1	time					359:362	activated partial thromboplastin time	326:362	activated partial thromboplastin time (APTT)	326:369	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	2	46	theme	thromboplastin	344:357	arg1	APTT					365:368	APTT	365:368	APTT	365:368	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	6	47	theme	anticoagulant	1189:1201	arg1	candidates					1203:1212	promising anticoagulant candidates	1179:1212	promising anticoagulant candidates	1179:1212	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	6	47	theme	anticoagulant	1189:1201	arg1	polysaccharides					1139:1153	acidic polysaccharides	1132:1153	acidic polysaccharides from scallop viscera	1132:1174	The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.					
31220498	2	48	theme	partial	336:342	arg1	time					359:362	activated partial thromboplastin time	326:362	activated partial thromboplastin time (APTT)	326:369	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	2	48	theme	partial	336:342	arg1	APTT					365:368	APTT	365:368	APTT	365:368	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	0	49	theme	Patinopecten	83:94	arg1	viscera					107:113	Patinopecten yessoensis viscera	83:113	Patinopecten yessoensis viscera	83:113	Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.					
31220498	4	50	theme	composition	767:777	arg1	analysis					779:786	monosaccharide composition analysis	752:786	monosaccharide composition analysis	752:786	Based on monosaccharide composition analysis, methylation analysis, and NMR analysis.					
31220498	2	51	theme	thrombin	375:382	arg1	TT					390:391	TT	390:391	TT	390:391	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	2	51	theme	thrombin	375:382	arg1	time					384:387	thrombin time	375:387	thrombin time (TT)	375:392	Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways.					
31220498	3	52	theme	1 → 2	683:687	arg1	backbone					644:651	its backbone	640:651	its backbone	640:651	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	3	52	theme	1 → 2	683:687	arg1	Xyl					706:708	→6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc	671:715	Xyl	706:708	Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal.					
31220498	4	53	theme	monosaccharide	752:765	arg1	analysis					779:786	monosaccharide composition analysis	752:786	monosaccharide composition analysis	752:786	Based on monosaccharide composition analysis, methylation analysis, and NMR analysis.					
31220498	5	54	theme	monosaccharide	857:870	arg1	analysis					872:879	their monosaccharide analysis	851:879	their monosaccharide analysis	851:879	Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2.					
31220498	1	55	theme	DEAE-52	251:257	arg1	cellulose					259:267	DEAE-52 cellulose	251:267	DEAE-52 cellulose	251:267	In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B.					
31002629	0	0	theme	Antitumor	73:81	arg1	Activity					83:90	In Vitro Antitumor Activity	64:90	In Vitro Antitumor Activity of Four Products of Cordyceps	64:120	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	1	1	theme	sinensis	324:331	arg1	mycelia					298:304	the dried fermented mycelia	278:304	the dried fermented mycelia of Cephalosporium sinensis	278:331	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	bodies					420:425	the dried fruiting bodies	401:425	the dried fruiting bodies of C. militaris (C4)	401:446	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	extracts					264:271	water extracts	258:271	water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1)	258:336	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	hepiali					352:358	Paecilomyces hepiali	339:358	Paecilomyces hepiali (C2)	339:363	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	cicadae					376:382	Cordyceps cicadae	366:382	Cordyceps cicadae (C3)	366:387	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	C3					385:386	C3	385:386	C3	385:386	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	C1					334:335	C1	334:335	C1	334:335	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	2	from	mycelia	298:304	arg1	C2					361:362	C2	361:362	C2	361:362	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	7	3	theme	extracts	1162:1169	arg1	activities					1127:1136	the antitumor activities	1113:1136	the antitumor activities of the respective water extracts	1113:1169	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	3	4	theme	Na	679:680	arg1	contents					682:689	Na contents	679:689	Na contents	679:689	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	7	5	theme	antitumor	1117:1125	arg1	activities					1127:1136	the antitumor activities	1113:1136	the antitumor activities of the respective water extracts	1113:1169	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	5	6	from	content	897:903	arg1	C4					908:909	C4	908:909	C4	908:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	2	7	theme	C.	531:532	arg1	S1					545:546	S1	545:546	S1	545:546	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	2	7	theme	C.	531:532	arg1	militaris					534:542	natural C. militaris	523:542	natural C. militaris (S1)	523:547	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	7	8	theme	>	1212:1212	arg1	order					1190:1194	the order	1186:1194	the order of C3 > C4 > C1 > C2	1186:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	4	9	located	detected	809:816	arg1	S1					830:831	S1	830:831	S1	830:831	Except for C4, cordycepin was not detected in C1-C3 or S1.					
31002629	4	9	located	detected	809:816	arg1	C1-C3					821:825	C1-C3	821:825	C1-C3	821:825	Except for C4, cordycepin was not detected in C1-C3 or S1.					
31002629	4	9	located	detected	809:816	arg2	cordycepin					790:799	cordycepin	790:799	cordycepin	790:799	Except for C4, cordycepin was not detected in C1-C3 or S1.					
31002629	7	10	theme	water	1156:1160	arg1	extracts					1162:1169	the respective water extracts	1141:1169	the respective water extracts	1141:1169	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	2	11	theme	natural	523:529	arg1	S1					545:546	S1	545:546	S1	545:546	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	2	11	theme	natural	523:529	arg1	militaris					534:542	natural C. militaris	523:542	natural C. militaris (S1)	523:547	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	0	12	theme	Activity	83:90	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes)	0:134	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	3	13	theme	Zn	570:571	arg1	contents					554:561	The contents	550:561	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	13	theme	Zn	570:571	arg1	different					636:644	different	636:644	different	636:644	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	13	theme	Zn	570:571	arg1	ratio					606:610	the Zn/Cu ratio	596:610	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	5	14	theme	total	878:882	arg1	content					897:903	total triterpenoid content	878:903	total triterpenoid content in C4	878:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	7	15	theme	respective	1145:1154	arg1	extracts					1162:1169	the respective water extracts	1141:1169	the respective water extracts	1141:1169	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	3	16	theme	K/Na	695:698	arg1	ratios					700:705	K/Na ratios	695:705	K/Na ratios	695:705	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	6	17	theme	highest	1051:1057	arg1	glucose					1069:1075	glucose	1069:1075	glucose	1069:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	17	theme	highest	1051:1057	arg1	amount					1059:1064	the highest amount	1047:1064	the highest amount of glucose	1047:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	0	18	theme	Products	100:107	arg1	Compositions					23:34	Chemical Compositions	14:34	Chemical Compositions	14:34	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	0	18	theme	Products	100:107	arg1	Ingredients					47:57	Bioactive Ingredients	37:57	Bioactive Ingredients	37:57	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	0	18	theme	Products	100:107	arg1	Activity					83:90	In Vitro Antitumor Activity	64:90	In Vitro Antitumor Activity of Four Products of Cordyceps	64:120	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	0	18	theme	Products	100:107	arg1	Ascomycetes					123:133	Ascomycetes	123:133	Ascomycetes	123:133	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	6	19	theme	polysaccharide	984:997	arg1	analysis					962:969	Detailed analysis	953:969	Detailed analysis of the crude polysaccharide from C4	953:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	20	from	analysis	962:969	arg1	C4					1004:1005	C4	1004:1005	C4	1004:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	5	21	from	contents	859:866	arg1	content					897:903	total triterpenoid content	878:903	total triterpenoid content in C4	878:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	5	21	from	contents	859:866	arg1	C4					871:872	C4	871:872	C4	871:872	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	1	22	theme	Paecilomyces	339:350	arg1	C2					361:362	C2	361:362	C2	361:362	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	22	theme	Paecilomyces	339:350	arg1	hepiali					352:358	Paecilomyces hepiali	339:358	Paecilomyces hepiali (C2)	339:363	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	5	23	theme	polysaccharide	844:857	arg1	contents					859:866	The crude polysaccharide contents	834:866	The crude polysaccharide contents in C4 and total triterpenoid content in C4	834:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	5	23	theme	polysaccharide	844:857	arg1	lower					927:931	lower	927:931	lower	927:931	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	7	24	dep	>	1212:1212	arg1	C2					1214:1215	C2	1214:1215	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	5	25	theme	crude	838:842	arg1	contents					859:866	The crude polysaccharide contents	834:866	The crude polysaccharide contents in C4 and total triterpenoid content in C4	834:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	5	25	theme	crude	838:842	arg1	lower					927:931	lower	927:931	lower	927:931	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	8	26	theme	strains	1284:1290	arg1	products					1262:1269	products	1262:1269	products of Cordyceps strains	1262:1290	These results can help us better understand products of Cordyceps strains.					
31002629	1	27	theme	chemical	160:167	arg1	compositions					169:180	The chemical compositions	156:180	The chemical compositions	156:180	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	28	theme	water	258:262	arg1	C1					334:335	C1	334:335	C1	334:335	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	28	theme	water	258:262	arg1	extracts					264:271	water extracts	258:271	water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1)	258:336	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	29	theme	dried	405:409	arg1	bodies					420:425	the dried fruiting bodies	401:425	the dried fruiting bodies of C. militaris (C4)	401:446	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	7	30	theme	C4	1204:1205	arg1	>					1212:1212	C3 > C4 > C1 >	1199:1212	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	3	31	from	those	755:759	arg1	different					740:748	different	740:748	different	740:748	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	0	32	theme	Compositions	23:34	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes)	0:134	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	3	33	theme	Ca	582:583	arg1	contents					554:561	The contents	550:561	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	33	theme	Ca	582:583	arg1	different					636:644	different	636:644	different	636:644	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	33	theme	Ca	582:583	arg1	ratio					606:610	the Zn/Cu ratio	596:610	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	0	34	theme	Cordyceps	112:120	arg1	Products					100:107	Four Products	95:107	Four Products of Cordyceps	95:120	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	1	35	theme	fruiting	411:418	arg1	bodies					420:425	the dried fruiting bodies	401:425	the dried fruiting bodies of C. militaris (C4)	401:446	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	0	36	dep	In	64:65	arg1	Vitro					67:71	Vitro	67:71	Vitro	67:71	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	0	37	theme	Chemical	14:21	arg1	Compositions					23:34	Chemical Compositions	14:34	Chemical Compositions	14:34	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	0	37	theme	Chemical	14:21	arg1	Ascomycetes					123:133	Ascomycetes	123:133	Ascomycetes	123:133	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	3	38	theme	Mn	574:575	arg1	contents					554:561	The contents	550:561	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	38	theme	Mn	574:575	arg1	different					636:644	different	636:644	different	636:644	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	38	theme	Mn	574:575	arg1	ratio					606:610	the Zn/Cu ratio	596:610	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	2	39	theme	acid	461:464	arg1	content					466:472	Total amino acid content	449:472	Total amino acid content	449:472	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	1	40	theme	bioactive	183:191	arg1	ingredients					193:203	bioactive ingredients	183:203	bioactive ingredients	183:203	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	0	41	theme	Bioactive	37:45	arg1	Ingredients					47:57	Bioactive Ingredients	37:57	Bioactive Ingredients	37:57	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	2	42	theme	amino	455:459	arg1	content					466:472	Total amino acid content	449:472	Total amino acid content	449:472	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	5	43	from	C4	871:872	arg1	C4					908:909	C4	908:909	C4	908:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	1	44	theme	dried	282:286	arg1	mycelia					298:304	the dried fermented mycelia	278:304	the dried fermented mycelia of Cephalosporium sinensis	278:331	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	2	45	theme	Total	449:453	arg1	content					466:472	Total amino acid content	449:472	Total amino acid content	449:472	Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1).					
31002629	7	46	theme	C1	1209:1210	arg1	>					1212:1212	C3 > C4 > C1 >	1199:1212	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	6	47	theme	Detailed	953:960	arg1	analysis					962:969	Detailed analysis	953:969	Detailed analysis of the crude polysaccharide from C4	953:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	3	48	from	contents	554:561	arg1	C1-C3					615:619	C1-C3	615:619	C1-C3	615:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	1	49	theme	fermented	288:296	arg1	mycelia					298:304	the dried fermented mycelia	278:304	the dried fermented mycelia of Cephalosporium sinensis	278:331	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	50	dep	in	210:211	arg1	vitro					213:217	vitro	213:217	vitro	213:217	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	3	51	theme	Fe	566:567	arg1	contents					554:561	The contents	550:561	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	51	theme	Fe	566:567	arg1	different					636:644	different	636:644	different	636:644	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	51	theme	Fe	566:567	arg1	ratio					606:610	the Zn/Cu ratio	596:610	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	0	52	theme	Ingredients	47:57	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes)	0:134	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	5	53	theme	triterpenoid	884:895	arg1	content					897:903	total triterpenoid content	878:903	total triterpenoid content in C4	878:909	The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3.					
31002629	3	54	from	ratio	606:610	arg1	C1-C3					615:619	C1-C3	615:619	C1-C3	615:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	1	55	theme	Cordyceps	366:374	arg1	cicadae					376:382	Cordyceps cicadae	366:382	Cordyceps cicadae (C3)	366:387	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	55	theme	Cordyceps	366:374	arg1	C3					385:386	C3	385:386	C3	385:386	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	6	56	theme	crude	978:982	arg1	polysaccharide					984:997	the crude polysaccharide	974:997	the crude polysaccharide from C4	974:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	7	57	theme	>	1207:1207	arg1	>					1212:1212	C3 > C4 > C1 >	1199:1212	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	3	58	from	contents	682:689	arg1	S1					722:723	S1	722:723	S1	722:723	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	58	from	contents	682:689	arg1	C3					710:711	C3	710:711	C3	710:711	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	58	from	contents	682:689	arg1	C4					714:715	C4	714:715	C4	714:715	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	1	59	theme	militaris	433:441	arg1	bodies					420:425	the dried fruiting bodies	401:425	the dried fruiting bodies of C. militaris (C4)	401:446	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	extracts					264:271	water extracts	258:271	water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1)	258:336	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	hepiali					352:358	Paecilomyces hepiali	339:358	Paecilomyces hepiali (C2)	339:363	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	cicadae					376:382	Cordyceps cicadae	366:382	Cordyceps cicadae (C3)	366:387	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	C3					385:386	C3	385:386	C3	385:386	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	C1					334:335	C1	334:335	C1	334:335	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	1	59	theme	militaris	433:441	arg1	C2					361:362	C2	361:362	C2	361:362	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	6	60	contain	contained	1037:1045	arg2	amount					1059:1064	the highest amount	1047:1064	the highest amount of glucose	1047:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	60	contain	contained	1037:1045	arg1	it					1034:1035	it	1034:1035	it	1034:1035	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	60	contain	contained	1037:1045	arg2	glucose					1069:1075	glucose	1069:1075	glucose	1069:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	61	from	C4	1004:1005	arg1	polysaccharide					984:997	the crude polysaccharide	974:997	the crude polysaccharide from C4	974:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	61	from	C4	1004:1005	arg1	analysis					962:969	Detailed analysis	953:969	Detailed analysis of the crude polysaccharide from C4	953:1005	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	7	62	theme	C3	1199:1200	arg1	>					1212:1212	C3 > C4 > C1 >	1199:1212	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	6	63	theme	glucose	1069:1075	arg1	glucose					1069:1075	glucose	1069:1075	glucose	1069:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	6	63	theme	glucose	1069:1075	arg1	amount					1059:1064	the highest amount	1047:1064	the highest amount of glucose	1047:1075	Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3.					
31002629	1	64	theme	in	210:211	arg1	activity					229:236	in vitro antitumor activity	210:236	in vitro antitumor activity	210:236	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	0	65	theme	In	64:65	arg1	Activity					83:90	In Vitro Antitumor Activity	64:90	In Vitro Antitumor Activity of Four Products of Cordyceps	64:120	Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China.					
31002629	7	66	theme	>	1202:1202	arg1	>					1212:1212	C3 > C4 > C1 >	1199:1212	C3 > C4 > C1 > C2	1199:1215	Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2.					
31002629	3	67	theme	Zn/Cu	600:604	arg1	different					636:644	different	636:644	different	636:644	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	67	theme	Zn/Cu	600:604	arg1	ratio					606:610	the Zn/Cu ratio	596:610	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3	550:619	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	1	68	theme	Cephalosporium	309:322	arg1	sinensis					324:331	Cephalosporium sinensis	309:331	Cephalosporium sinensis	309:331	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
31002629	3	69	from	ratios	700:705	arg1	S1					722:723	S1	722:723	S1	722:723	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	69	from	ratios	700:705	arg1	C3					710:711	C3	710:711	C3	710:711	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	3	69	from	ratios	700:705	arg1	C4					714:715	C4	714:715	C4	714:715	The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2.					
31002629	8	70	theme	Cordyceps	1274:1282	arg1	strains					1284:1290	Cordyceps strains	1274:1290	Cordyceps strains	1274:1290	These results can help us better understand products of Cordyceps strains.					
31002629	1	71	theme	antitumor	219:227	arg1	activity					229:236	in vitro antitumor activity	210:236	in vitro antitumor activity	210:236	The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4).					
30832845	3	0	theme	molecular	515:523	arg1	weight					525:530	molecular weight	515:530	molecular weight cut off (MWCO) of 300 kDa (F300)	515:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	1	theme	weight	525:530	arg1	cut					532:534	molecular weight cut	515:534	molecular weight cut off (MWCO) of 300 kDa (F300)	515:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	1	theme	weight	525:530	arg1	MWCO					541:544	MWCO	541:544	MWCO	541:544	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	6	2	theme	antioxidant	969:979	arg1	capacity					981:988	secondary antioxidant capacity	959:988	secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA)	959:1036	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	0	3	theme	New	107:109	arg1	Zealand					111:117	New Zealand	107:117	New Zealand	107:117	Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand.					
30832845	6	4	theme	secondary	959:967	arg1	capacity					981:988	secondary antioxidant capacity	959:988	secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA)	959:1036	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	5	5	theme	FTIR	708:711	arg1	analysis					725:732	FTIR and 2D HSQC analysis	708:732	analysis	725:732	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	1	6	from	species	148:154	arg1	Fucoidan					120:127	Fucoidan	120:127	Fucoidan from brown seaweed species	120:154	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	6	from	species	148:154	arg1	pinnatifida					165:175	pinnatifida	165:175	pinnatifida	165:175	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	7	theme	seaweed	140:146	arg1	species					148:154	brown seaweed species	134:154	brown seaweed species	134:154	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	5	8	theme	alternating	797:807	arg1	backbone					826:833	an alternating fucose-galactose backbone	794:833	an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions	794:912	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	4	9	theme	higher	644:649	arg1	content					659:665	significantly higher sulfate content	630:665	significantly higher sulfate content	630:665	FStd contained significantly higher sulfate content as compared to Fcrude and F300.					
30832845	5	10	theme	2D	717:718	arg1	analysis					725:732	FTIR and 2D HSQC analysis	708:732	analysis	725:732	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	2	11	theme	research	281:288	arg1	objective					263:271	The objective	259:271	The objective of this research	259:288	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	3	12	theme	Crude	464:468	arg1	Fcrude					480:485	Fcrude	480:485	Fcrude	480:485	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	12	theme	Crude	464:468	arg1	fucoidan					470:477	Crude fucoidan	464:477	Crude fucoidan (Fcrude)	464:486	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	5	13	theme	purified	760:767	arg1	fucoidan					769:776	the purified fucoidan	756:776	the purified fucoidan	756:776	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	2	14	attach	isolated	343:350	arg2	fucoidan					334:341	fucoidan	334:341	fucoidan isolated from U. pinnatifida harvested in New Zealand	334:395	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	2	14	attach	isolated	343:350	arg1	pinnatifida					360:370	pinnatifida	360:370	pinnatifida	360:370	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	3	15	with	fucoidan	501:508	arg1	cut					532:534	molecular weight cut	515:534	molecular weight cut off (MWCO) of 300 kDa (F300)	515:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	15	with	fucoidan	501:508	arg1	MWCO					541:544	MWCO	541:544	MWCO	541:544	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	5	16	theme	C4	901:902	arg1	positions					904:912	C2 and C4 positions	894:912	C2 and C4 positions	894:912	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	1	17	theme	numerous	182:189	arg1	anticancer					236:245	anticancer	236:245	anticancer	236:245	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	17	theme	numerous	182:189	arg1	properties					201:210	numerous bioactive properties	182:210	numerous bioactive properties such as antioxidant and anticancer activities	182:256	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	17	theme	numerous	182:189	arg1	antioxidant					220:230	antioxidant	220:230	antioxidant	220:230	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	18	theme	bioactive	191:199	arg1	anticancer					236:245	anticancer	236:245	anticancer	236:245	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	18	theme	bioactive	191:199	arg1	properties					201:210	numerous bioactive properties	182:210	numerous bioactive properties such as antioxidant and anticancer activities	182:256	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	18	theme	bioactive	191:199	arg1	antioxidant					220:230	antioxidant	220:230	antioxidant	220:230	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	0	19	theme	antioxidant	31:41	arg1	activity					43:50	antioxidant activity	31:50	antioxidant activity	31:50	Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand.					
30832845	6	20	theme	New	937:939	arg1	Zealand					941:947	New Zealand	937:947	New Zealand	937:947	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	3	21	with	fucoidan	470:477	arg1	cut					532:534	molecular weight cut	515:534	molecular weight cut off (MWCO) of 300 kDa (F300)	515:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	21	with	fucoidan	470:477	arg1	MWCO					541:544	MWCO	541:544	MWCO	541:544	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	22	theme	300 kDa	550:556	arg1	cut					532:534	molecular weight cut	515:534	molecular weight cut off (MWCO) of 300 kDa (F300)	515:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	22	theme	300 kDa	550:556	arg1	MWCO					541:544	MWCO	541:544	MWCO	541:544	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	2	23	theme	antioxidant	442:452	arg1	capacity					454:461	antioxidant capacity	442:461	antioxidant capacity	442:461	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	5	24	attach	linked	835:840	arg3	bonds					870:874	1,3 glycosidic bonds	855:874	1,3 glycosidic bonds	855:874	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	5	25	theme	1,3	855:857	arg1	bonds					870:874	1,3 glycosidic bonds	855:874	1,3 glycosidic bonds	855:874	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	6	26	from	Zealand	941:947	arg1	samples					924:930	Fucoidan samples	915:930	Fucoidan samples from New Zealand	915:947	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	4	27	contain	contained	620:628	arg1	FStd					615:618	FStd	615:618	FStd	615:618	FStd contained significantly higher sulfate content as compared to Fcrude and F300.					
30832845	4	27	contain	contained	620:628	arg2	content					659:665	significantly higher sulfate content	630:665	significantly higher sulfate content	630:665	FStd contained significantly higher sulfate content as compared to Fcrude and F300.					
30832845	4	28	theme	sulfate	651:657	arg1	content					659:665	significantly higher sulfate content	630:665	significantly higher sulfate content	630:665	FStd contained significantly higher sulfate content as compared to Fcrude and F300.					
30832845	2	29	theme	molecular	418:426	arg1	structure					428:436	molecular structure	418:436	molecular structure	418:436	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	6	30	theme	low-molecular-weight	1044:1063	arg1	fucoidan					1065:1072	low-molecular-weight fucoidan	1044:1072	low-molecular-weight fucoidan	1044:1072	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	6	31	theme	Fucoidan	915:922	arg1	samples					924:930	Fucoidan samples	915:930	Fucoidan samples from New Zealand	915:947	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	1	32	contain	has	178:180	arg2	antioxidant					220:230	antioxidant	220:230	antioxidant	220:230	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	32	contain	has	178:180	arg1	Fucoidan					120:127	Fucoidan	120:127	Fucoidan from brown seaweed species	120:154	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	32	contain	has	178:180	arg2	properties					201:210	numerous bioactive properties	182:210	numerous bioactive properties such as antioxidant and anticancer activities	182:256	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	32	contain	has	178:180	arg2	anticancer					236:245	anticancer	236:245	anticancer	236:245	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	1	32	contain	has	178:180	arg1	pinnatifida					165:175	pinnatifida	165:175	pinnatifida	165:175	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	5	33	theme	fucose-galactose	809:824	arg1	backbone					826:833	an alternating fucose-galactose backbone	794:833	an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions	794:912	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	6	34	theme	higher	1092:1097	arg1	capacity					1099:1106	significantly higher capacity	1078:1106	significantly higher capacity	1078:1106	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	0	35	theme	fucoidan	55:62	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand.					
30832845	0	35	theme	fucoidan	55:62	arg1	activity					43:50	antioxidant activity	31:50	antioxidant activity	31:50	Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand.					
30832845	5	36	from	positions	904:912	arg1	sulfation					881:889	sulfation	881:889	sulfation at C2 and C4 positions	881:912	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	3	37	theme	standard	598:605	arg1	fucoidan					589:596	a fucoidan standard	587:605	a fucoidan standard (FStd)	587:612	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	3	37	theme	standard	598:605	arg1	FStd					608:611	FStd	608:611	FStd	608:611	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	6	38	contain	had	1074:1076	arg1	fucoidan					1065:1072	low-molecular-weight fucoidan	1044:1072	low-molecular-weight fucoidan	1044:1072	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	6	38	contain	had	1074:1076	arg2	capacity					1099:1106	significantly higher capacity	1078:1106	significantly higher capacity	1078:1106	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	1	39	theme	brown	134:138	arg1	species					148:154	brown seaweed species	134:154	brown seaweed species	134:154	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	2	40	theme	chemical	310:317	arg1	composition					319:329	the chemical composition	306:329	the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand	306:395	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	1	41	dep	antioxidant	220:230	arg1	activities					247:256	activities	247:256	activities	247:256	Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities.					
30832845	2	42	theme	fucoidan	334:341	arg1	composition					319:329	the chemical composition	306:329	the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand	306:395	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	5	43	theme	C2	894:895	arg1	positions					904:912	C2 and C4 positions	894:912	C2 and C4 positions	894:912	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	5	44	theme	HSQC	720:723	arg1	analysis					725:732	FTIR and 2D HSQC analysis	708:732	analysis	725:732	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
30832845	6	45	theme	butylated	1007:1015	arg1	BHA					1033:1035	BHA	1033:1035	BHA	1033:1035	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	6	45	theme	butylated	1007:1015	arg1	hydroxyanisole					1017:1030	butylated hydroxyanisole	1007:1030	butylated hydroxyanisole (BHA)	1007:1036	Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.					
30832845	2	46	theme	New	385:387	arg1	Zealand					389:395	New Zealand	385:395	New Zealand	385:395	The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity.					
30832845	3	47	theme	purified	492:499	arg1	fucoidan					501:508	purified fucoidan	492:508	purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300)	492:563	Crude fucoidan (Fcrude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F300) were compared against a fucoidan standard (FStd).					
30832845	0	48	dep	Structure	0:8	arg1	characterization					10:25	characterization	10:25	characterization	10:25	Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand.					
30832845	5	49	theme	glycosidic	859:868	arg1	bonds					870:874	1,3 glycosidic bonds	855:874	1,3 glycosidic bonds	855:874	Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions.					
31712146	6	0	theme	acid	829:832	arg1	yield					783:787	the highest extraction yield	760:787	the highest extraction yield	760:787	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	0	theme	acid	829:832	arg1	contents					794:801	the contents	790:801	the contents of total sugar and uronic acid	790:832	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	0	theme	acid	829:832	arg1	weight					861:866	the smallest molecular weight	838:866	the smallest molecular weight	838:866	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	5	1	theme	element	611:617	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	1	theme	element	611:617	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	8	2	from	increase	1094:1101	arg1	P <0 .05					1194:1201	P <0 .05	1194:1201	P <0 .05	1194:1201	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	2	from	increase	1094:1101	arg1	rats					1188:1191	diabetic rats	1179:1191	diabetic rats (P <0 .05)	1179:1202	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	7	3	contain	had	920:922	arg2	structure					940:948	no triple-helix structure	924:948	no triple-helix structure	924:948	Congo red assay indicated that all polysaccharides had no triple-helix structure.					
31712146	7	3	contain	had	920:922	arg1	polysaccharides					904:918	all polysaccharides	900:918	all polysaccharides	900:918	Congo red assay indicated that all polysaccharides had no triple-helix structure.					
31712146	11	4	theme	increased	1493:1501	arg1	contents					1515:1522	the increased ALT and BUN contents	1489:1522	the increased ALT and BUN contents caused by the liver and kidney damages	1489:1561	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	11	4	theme	increased	1493:1501	arg1	Meanwhile					1478:1486	Meanwhile	1478:1486	Meanwhile	1478:1486	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	5	5	theme	molecular	620:628	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	5	theme	molecular	620:628	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	6	6	theme	total	806:810	arg1	sugar					812:816	total sugar	806:816	total sugar	806:816	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	10	7	theme	low	1400:1402	arg1	lipoprotein					1412:1422	the low density lipoprotein	1396:1422	the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05)	1396:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	12	8	theme	diabetes	1760:1767	arg1	improvement					1745:1755	the improvement	1741:1755	the improvement of diabetes and its complications	1741:1789	As a consequence, APs could be promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications.					
31712146	11	9	theme	APs	1615:1617	arg1	P < .05					1633:1639	P < .05	1633:1639	P < .05	1633:1639	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	11	9	theme	APs	1615:1617	arg1	intervention					1619:1630	APs intervention	1615:1630	APs intervention (P < .05)	1615:1640	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	4	10	theme	water	306:310	arg1	extraction					312:321	hot water extraction	302:321	hot water extraction	302:321	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	8	11	theme	cholesterol	1159:1169	arg1	levels					1106:1111	levels	1106:1111	levels of blood glucose, triglyceride (TG) and total cholesterol (TC)	1106:1174	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	12	theme	glucose	1122:1128	arg1	levels					1106:1111	levels	1106:1111	levels of blood glucose, triglyceride (TG) and total cholesterol (TC)	1106:1174	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	10	13	theme	rats	1462:1465	arg1	level					1444:1448	the low density lipoprotein cholesterol (LDL-C) level	1396:1448	the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05)	1396:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	7	14	theme	triple-helix	927:938	arg1	structure					940:948	no triple-helix structure	924:948	no triple-helix structure	924:948	Congo red assay indicated that all polysaccharides had no triple-helix structure.					
31712146	8	15	theme	triglyceride	1131:1142	arg1	levels					1106:1111	levels	1106:1111	levels of blood glucose, triglyceride (TG) and total cholesterol (TC)	1106:1174	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	6	16	theme	molecular	851:859	arg1	weight					861:866	the smallest molecular weight	838:866	the smallest molecular weight	838:866	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	1	17	theme	Sargassum	221:229	arg1	Scagassum					204:212	Scagassum	204:212	Scagassum (SCP)	204:218	Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.)					
31712146	1	17	theme	Sargassum	221:229	arg1	fusiforme					231:239	Sargassum fusiforme	221:239	Sargassum fusiforme	221:239	Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.)					
31712146	4	18	theme	antidiabetic	366:377	arg1	activity					379:386	antidiabetic activity	366:386	antidiabetic activity	366:386	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	11	19	theme	kidney	1548:1553	arg1	damages					1555:1561	the liver and kidney damages	1534:1561	the liver and kidney damages	1534:1561	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	5	20	theme	uronic	707:712	arg1	acid					714:717	uronic acid	707:717	uronic acid	707:717	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	1	21	theme	algal	157:161	arg1	APs					180:182	APs	180:182	APs	180:182	Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.)					
31712146	1	21	theme	algal	157:161	arg1	polysaccharides					163:177	Three algal polysaccharides	151:177	Three algal polysaccharides (APs)	151:183	Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.)					
31712146	10	22	theme	lipoprotein	1412:1422	arg1	LDL-C					1437:1441	LDL-C	1437:1441	LDL-C	1437:1441	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	10	22	theme	lipoprotein	1412:1422	arg1	cholesterol					1424:1434	the low density lipoprotein cholesterol	1396:1434	the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05)	1396:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	0	23	theme	hypoglycemic	84:95	arg1	activities					115:124	their hypoglycemic and hypolipidemic activities	78:124	their hypoglycemic and hypolipidemic activities in type 2 diabetic rats	78:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	7	24	theme	red	875:877	arg1	assay					879:883	Congo red assay	869:883	Congo red assay	869:883	Congo red assay indicated that all polysaccharides had no triple-helix structure.					
31712146	4	25	theme	diabetic	457:464	arg1	rats					466:469	streptozotocin-induced type 2 diabetic rats	427:469	streptozotocin-induced type 2 diabetic rats	427:469	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	0	26	theme	hypolipidemic	101:113	arg1	activities					115:124	their hypoglycemic and hypolipidemic activities	78:124	their hypoglycemic and hypolipidemic activities in type 2 diabetic rats	78:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	4	27	theme	fat	414:416	arg1	diet					418:421	high fat diet	409:421	high fat diet	409:421	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	5	28	theme	protein	698:704	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	28	theme	protein	698:704	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	8	29	theme	APs	974:976	arg1	administration					956:969	Oral administration	951:969	Oral administration of APs	951:976	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	0	30	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of polysaccharides from three seaweed species	0:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	9	31	theme	Diabetic	1205:1212	arg1	rats					1214:1217	Diabetic rats	1205:1217	Diabetic rats treated with SCP	1205:1234	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	12	32	theme	natural	1698:1704	arg1	medicines					1706:1714	natural medicines	1698:1714	natural medicines	1698:1714	As a consequence, APs could be promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications.					
31712146	5	33	theme	extraction	532:541	arg1	yield					543:547	extraction yield	532:547	extraction yield	532:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	10	34	theme	positive	1367:1374	arg1	effect					1376:1381	positive effect	1367:1381	positive effect	1367:1381	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	11	35	theme	BUN	1511:1513	arg1	contents					1515:1522	the increased ALT and BUN contents	1489:1522	the increased ALT and BUN contents caused by the liver and kidney damages	1489:1561	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	11	35	theme	BUN	1511:1513	arg1	Meanwhile					1478:1486	Meanwhile	1478:1486	Meanwhile	1478:1486	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	0	36	theme	seaweed	58:64	arg1	species					66:72	three seaweed species	52:72	three seaweed species	52:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	6	37	theme	highest	764:770	arg1	yield					783:787	the highest extraction yield	760:787	the highest extraction yield	760:787	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	9	38	theme	high	1252:1255	arg1	HDL-C					1290:1294	HDL-C	1290:1294	HDL-C	1290:1294	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	38	theme	high	1252:1255	arg1	cholesterol					1277:1287	high density lipoprotein cholesterol	1252:1287	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	8	39	theme	intake	1038:1043	arg1	increase					1020:1027	increase	1020:1027	increase of water intake (P < .05)	1020:1053	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	39	theme	intake	1038:1043	arg1	loss					1001:1004	loss	1001:1004	loss of weight	1001:1014	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	9	40	theme	lipoprotein	1265:1275	arg1	HDL-C					1290:1294	HDL-C	1290:1294	HDL-C	1290:1294	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	40	theme	lipoprotein	1265:1275	arg1	cholesterol					1277:1287	high density lipoprotein cholesterol	1252:1287	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	5	41	theme	organic	603:609	arg1	element					611:617	organic element	603:617	organic element	603:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	6	42	theme	uronic	822:827	arg1	acid					829:832	uronic acid	822:832	uronic acid	822:832	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	12	43	theme	functional	1720:1729	arg1	foods					1731:1735	functional foods	1720:1735	functional foods	1720:1735	As a consequence, APs could be promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications.					
31712146	10	44	theme	cholesterol	1424:1434	arg1	level					1444:1448	the low density lipoprotein cholesterol (LDL-C) level	1396:1448	the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05)	1396:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	6	45	theme	sugar	812:816	arg1	yield					783:787	the highest extraction yield	760:787	the highest extraction yield	760:787	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	45	theme	sugar	812:816	arg1	contents					794:801	the contents	790:801	the contents of total sugar and uronic acid	790:832	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	45	theme	sugar	812:816	arg1	weight					861:866	the smallest molecular weight	838:866	the smallest molecular weight	838:866	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	4	46	theme	structural	334:343	arg1	characterization					345:360	their structural characterization	328:360	their structural characterization	328:360	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	10	47	theme	density	1404:1410	arg1	lipoprotein					1412:1422	the low density lipoprotein	1396:1422	the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05)	1396:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	11	48	theme	ALT	1503:1505	arg1	contents					1515:1522	the increased ALT and BUN contents	1489:1522	the increased ALT and BUN contents caused by the liver and kidney damages	1489:1561	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	11	48	theme	ALT	1503:1505	arg1	Meanwhile					1478:1486	Meanwhile	1478:1486	Meanwhile	1478:1486	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	4	49	theme	hot	302:304	arg1	extraction					312:321	hot water extraction	302:321	hot water extraction	302:321	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	5	50	theme	monosaccharide	584:597	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	50	theme	monosaccharide	584:597	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	0	51	theme	diabetic	136:143	arg1	rats					145:148	type 2 diabetic rats	129:148	type 2 diabetic rats	129:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	8	52	theme	levels	1106:1111	arg1	increase					1094:1101	the increase	1090:1101	the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05)	1090:1202	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	53	theme	total	1153:1157	arg1	cholesterol					1159:1169	total cholesterol	1153:1169	total cholesterol (TC)	1153:1174	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	53	theme	total	1153:1157	arg1	TC					1172:1173	TC	1172:1173	TC	1172:1173	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	54	theme	blood	1116:1120	arg1	glucose					1122:1128	blood glucose	1116:1128	blood glucose	1116:1128	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	4	55	theme	high	409:412	arg1	diet					418:421	high fat diet	409:421	high fat diet	409:421	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	10	56	theme	diabetic	1453:1460	arg1	P < .05					1468:1474	P < .05	1468:1474	P < .05	1468:1474	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	10	56	theme	diabetic	1453:1460	arg1	rats					1462:1465	diabetic rats	1453:1465	diabetic rats (P < .05)	1453:1475	MAP and SCP showed positive effect in improving the low density lipoprotein cholesterol (LDL-C) level of diabetic rats (P < .05).					
31712146	9	57	theme	normal	1334:1339	arg1	group					1341:1345	normal group	1334:1345	normal group	1334:1345	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	6	58	theme	smallest	842:849	arg1	weight					861:866	the smallest molecular weight	838:866	the smallest molecular weight	838:866	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	5	59	theme	rheological	642:652	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	59	theme	rheological	642:652	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	60	theme	obvious	509:515	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	61	from	differences	517:527	arg1	yield					543:547	extraction yield	532:547	extraction yield	532:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	0	62	from	activities	115:124	arg1	rats					145:148	type 2 diabetic rats	129:148	type 2 diabetic rats	129:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	0	62	from	activities	115:124	arg1	species					66:72	three seaweed species	52:72	three seaweed species	52:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	8	63	theme	diabetic	1179:1186	arg1	P <0 .05					1194:1201	P <0 .05	1194:1201	P <0 .05	1194:1201	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	63	theme	diabetic	1179:1186	arg1	rats					1188:1191	diabetic rats	1179:1191	diabetic rats (P <0 .05)	1179:1202	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	12	64	theme	complications	1777:1789	arg1	improvement					1745:1755	the improvement	1741:1755	the improvement of diabetes and its complications	1741:1789	As a consequence, APs could be promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications.					
31712146	6	65	contain	had	756:758	arg2	contents					794:801	the contents	790:801	the contents of total sugar and uronic acid	790:832	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	65	contain	had	756:758	arg1	SFP					752:754	SFP	752:754	SFP	752:754	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	65	contain	had	756:758	arg2	weight					861:866	the smallest molecular weight	838:866	the smallest molecular weight	838:866	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	6	65	contain	had	756:758	arg2	yield					783:787	the highest extraction yield	760:787	the highest extraction yield	760:787	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	5	66	theme	sugar	691:695	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	66	theme	sugar	691:695	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	67	theme	acid	714:717	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	8	68	theme	Oral	951:954	arg1	administration					956:969	Oral administration	951:969	Oral administration of APs	951:976	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	0	69	from	characterization	11:26	arg1	rats					145:148	type 2 diabetic rats	129:148	type 2 diabetic rats	129:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	0	69	from	characterization	11:26	arg1	species					66:72	three seaweed species	52:72	three seaweed species	52:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	11	70	theme	diabetic	1596:1603	arg1	rats					1605:1608	the diabetic rats	1592:1608	the diabetic rats	1592:1608	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	5	71	theme	total	685:689	arg1	sugar					691:695	total sugar	685:695	total sugar	685:695	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	72	theme	molecular	550:558	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	72	theme	molecular	550:558	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	0	73	from	species	66:72	arg1	activities					115:124	their hypoglycemic and hypolipidemic activities	78:124	their hypoglycemic and hypolipidemic activities in type 2 diabetic rats	78:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	0	73	from	species	66:72	arg1	polysaccharides					31:45	polysaccharides	31:45	polysaccharides from three seaweed species	31:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	0	73	from	species	66:72	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of polysaccharides from three seaweed species	0:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	7	74	theme	Congo	869:873	arg1	assay					879:883	Congo red assay	869:883	Congo red assay	869:883	Congo red assay indicated that all polysaccharides had no triple-helix structure.					
31712146	4	75	theme	streptozotocin-induced	427:448	arg1	rats					466:469	streptozotocin-induced type 2 diabetic rats	427:469	streptozotocin-induced type 2 diabetic rats	427:469	(MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats.					
31712146	0	76	theme	polysaccharides	31:45	arg1	activities					115:124	their hypoglycemic and hypolipidemic activities	78:124	their hypoglycemic and hypolipidemic activities in type 2 diabetic rats	78:148	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	0	76	theme	polysaccharides	31:45	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of polysaccharides from three seaweed species	0:72	Structural characterization of polysaccharides from three seaweed species and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats.					
31712146	8	77	theme	weight	1009:1014	arg1	increase					1020:1027	increase	1020:1027	increase of water intake (P < .05)	1020:1053	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	77	theme	weight	1009:1014	arg1	loss					1001:1004	loss	1001:1004	loss of weight	1001:1014	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	5	78	theme	sulfate	723:729	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	78	theme	sulfate	723:729	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	12	79	theme	promising	1674:1682	arg1	candidates					1684:1693	promising candidates	1674:1693	promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications	1674:1789	As a consequence, APs could be promising candidates as natural medicines and functional foods for the improvement of diabetes and its complications.					
31712146	3	80	dep	Ag	289:290	arg1	L.					286:287	L.	286:287	L.	286:287	(SFP) and Macrocystis pyrifera(L.)Ag.					
31712146	3	80	dep	Ag	289:290	arg1	pyrifera					277:284	pyrifera	277:284	pyrifera	277:284	(SFP) and Macrocystis pyrifera(L.)Ag.					
31712146	1	81	dep	acquired	190:197	arg1	Harv					241:244	Harv	241:244	Harv	241:244	Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.)					
31712146	5	82	theme	SCP	734:736	arg1	compositions					568:579	compositions	568:579	compositions of monosaccharide and organic element	568:617	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	contents					673:680	the contents	669:680	the contents of total sugar, protein, uronic acid and sulfate	669:729	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	morphology					630:639	molecular morphology	620:639	molecular morphology	620:639	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	MAP					739:741	MAP	739:741	MAP	739:741	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	properties					654:663	rheological properties	642:663	rheological properties	642:663	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	SFP					747:749	SFP	747:749	SFP	747:749	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	weight					560:565	molecular weight	550:565	molecular weight	550:565	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	5	82	theme	SCP	734:736	arg1	differences					517:527	obvious differences	509:527	obvious differences in extraction yield	509:547	The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, rheological properties and the contents of total sugar, protein, uronic acid and sulfate of SCP, MAP and SFP.					
31712146	8	83	theme	water	1032:1036	arg1	P < .05					1046:1052	P < .05	1046:1052	P < .05	1046:1052	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	8	83	theme	water	1032:1036	arg1	intake					1038:1043	water intake	1032:1043	water intake (P < .05)	1032:1053	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	6	84	theme	extraction	772:781	arg1	yield					783:787	the highest extraction yield	760:787	the highest extraction yield	760:787	SFP had the highest extraction yield, the contents of total sugar and uronic acid and the smallest molecular weight.					
31712146	11	85	theme	liver	1538:1542	arg1	damages					1555:1561	the liver and kidney damages	1534:1561	the liver and kidney damages	1534:1561	Meanwhile, the increased ALT and BUN contents caused by the liver and kidney damages were markedly ameliorated in the diabetic rats with APs intervention (P < .05).					
31712146	9	86	theme	highest	1244:1250	arg1	P < .05					1304:1310	P < .05	1304:1310	P < .05	1304:1310	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	86	theme	highest	1244:1250	arg1	level					1297:1301	the highest high density lipoprotein cholesterol (HDL-C) level	1240:1301	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	87	theme	density	1257:1263	arg1	HDL-C					1290:1294	HDL-C	1290:1294	HDL-C	1290:1294	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	87	theme	density	1257:1263	arg1	cholesterol					1277:1287	high density lipoprotein cholesterol	1252:1287	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	8	88	dep	restrained	990:999	arg1	controlled					1079:1088	controlled	1079:1088	controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05)	1079:1202	Oral administration of APs prominently restrained loss of weight and increase of water intake (P < .05), and also significantly controlled the increase of levels of blood glucose, triglyceride (TG) and total cholesterol (TC) in diabetic rats (P <0 .05).					
31712146	9	89	contain	had	1236:1238	arg2	level					1297:1301	the highest high density lipoprotein cholesterol (HDL-C) level	1240:1301	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	89	contain	had	1236:1238	arg1	rats					1214:1217	Diabetic rats	1205:1217	Diabetic rats treated with SCP	1205:1234	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	89	contain	had	1236:1238	arg2	P < .05					1304:1310	P < .05	1304:1310	P < .05	1304:1310	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	90	theme	cholesterol	1277:1287	arg1	P < .05					1304:1310	P < .05	1304:1310	P < .05	1304:1310	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
31712146	9	90	theme	cholesterol	1277:1287	arg1	level					1297:1301	the highest high density lipoprotein cholesterol (HDL-C) level	1240:1301	the highest high density lipoprotein cholesterol (HDL-C) level (P < .05)	1240:1311	Diabetic rats treated with SCP had the highest high density lipoprotein cholesterol (HDL-C) level (P < .05), similar to those in normal group.					
30891034	6	0	theme	abundant	1074:1081	arg1	clostridiales					1054:1066	the orders clostridiales	1043:1066	the orders clostridiales (most abundant)	1043:1082	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	2	1	theme	inflammatory	357:368	arg1	conditions					370:379	autoimmune and inflammatory conditions	342:379	autoimmune and inflammatory conditions	342:379	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	12	2	theme	anti-carbohydrate	2034:2050	arg1	antibodies					2052:2061	natural anti-carbohydrate antibodies	2026:2061	natural anti-carbohydrate antibodies	2026:2061	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	9	3	theme	anti-glycan	1669:1679	arg1	antibodies					1681:1690	natural anti-glycan antibodies	1661:1690	natural anti-glycan antibodies	1661:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	11	4	theme	mice	1990:1993	arg1	microbiome					1968:1977	the gut microbiome	1960:1977	the gut microbiome of GalT-KO mice	1960:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	4	5	dep	regions	797:803	arg1	V3-V5					805:809	V3-V5	805:809	V3-V5	805:809	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	6	6	from	mice	1247:1250	arg1	development					1166:1176	the development	1162:1176	the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice	1162:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	13	7	theme	immune	2363:2368	arg1	responses					2370:2378	different immune responses	2353:2378	different immune responses to pathogens or other potential threats	2353:2418	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	11	8	theme	functional	1939:1948	arg1	output					1950:1955	the overall functional output	1927:1955	the overall functional output of the gut microbiome of GalT-KO mice	1927:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	12	9	theme	mouse	2188:2192	arg1	population					2161:2170	the gut bacterial population	2143:2170	the gut bacterial population of each GalT-KO mouse	2143:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	3	10	theme	immune	540:545	arg1	system					547:552	the host immune system	531:552	the host immune system	531:552	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	5	11	theme	antibodies	855:864	arg1	antibodies					855:864	glycan-specific natural antibodies	831:864	glycan-specific natural antibodies	831:864	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	5	11	theme	antibodies	855:864	arg1	repertoire					817:826	The repertoire	813:826	The repertoire	813:826	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	7	12	theme	anti-glycan	1386:1396	arg1	antibodies					1398:1407	natural anti-glycan antibodies	1378:1407	natural anti-glycan antibodies	1378:1407	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	13	13	theme	different	2353:2361	arg1	responses					2370:2378	different immune responses	2353:2378	different immune responses to pathogens or other potential threats	2353:2418	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	2	14	theme	disease	316:322	arg1	conditions					370:379	autoimmune and inflammatory conditions	342:379	autoimmune and inflammatory conditions	342:379	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	2	14	theme	disease	316:322	arg1	states					324:329	several disease states	308:329	several disease states	308:329	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	9	15	theme	examined	1721:1728	arg1	animals					1730:1736	the examined animals	1717:1736	the examined animals	1717:1736	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	1	16	theme	innate	190:195	arg1	homeostasis					217:227	the innate and adaptive immune homeostasis	186:227	the innate and adaptive immune homeostasis	186:227	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	6	17	theme	antibodies	1225:1234	arg1	repertoire					1191:1200	the final repertoire	1181:1200	the final repertoire of natural anti-glycan antibodies in GalT-KO mice	1181:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	6	17	theme	antibodies	1225:1234	arg1	antibodies					1225:1234	natural anti-glycan antibodies	1205:1234	natural anti-glycan antibodies	1205:1234	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	4	18	theme	taxons	647:652	arg1	abundance					625:633	abundance	625:633	abundance	625:633	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	18	theme	taxons	647:652	arg1	diversity					611:619	diversity	611:619	diversity	611:619	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	5	19	theme	glycan-specific	831:845	arg1	antibodies					855:864	glycan-specific natural antibodies	831:864	glycan-specific natural antibodies	831:864	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	8	20	theme	significant	1438:1448	arg1	associations					1472:1483	significant positive and negative associations	1438:1483	significant positive and negative associations	1438:1483	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	6	21	theme	natural	1205:1211	arg1	antibodies					1225:1234	natural anti-glycan antibodies	1205:1234	natural anti-glycan antibodies	1205:1234	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	10	22	theme	anti-glycan	1826:1836	arg1	antibodies					1838:1847	specific anti-glycan antibodies	1817:1847	specific anti-glycan antibodies	1817:1847	We also found redundancy in different taxa associated with the development of specific anti-glycan antibodies.					
30891034	1	23	theme	commensal	116:124	arg1	bacteria					126:133	Gut commensal bacteria	112:133	Gut commensal bacteria	112:133	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	7	24	theme	important	1332:1340	arg1	changes					1342:1348	important changes	1332:1348	important changes in levels and repertoire of natural anti-glycan antibodies in these mice	1332:1421	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	6	25	theme	repertoire	1191:1200	arg1	development					1166:1176	the development	1162:1176	the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice	1162:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	12	26	theme	gut	2147:2149	arg1	population					2161:2170	the gut bacterial population	2143:2170	the gut bacterial population of each GalT-KO mouse	2143:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	2	27	theme	intestinal	249:258	arg1	composition					270:280	the intestinal microbial composition	245:280	the intestinal microbial composition	245:280	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	4	28	theme	inbred	679:684	arg1	mice					694:697	inbred GalT-KO mice	679:697	inbred GalT-KO mice	679:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	29	from	diversity	611:619	arg1	microbiota					665:674	the gut microbiota	657:674	the gut microbiota of inbred GalT-KO mice	657:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	5	30	theme	printed	879:885	arg1	technology					900:909	printed glycan array technology	879:909	printed glycan array technology	879:909	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	4	31	theme	mice	694:697	arg1	microbiota					665:674	the gut microbiota	657:674	the gut microbiota of inbred GalT-KO mice	657:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	5	32	theme	array	894:898	arg1	technology					900:909	printed glycan array technology	879:909	printed glycan array technology	879:909	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	11	33	theme	GalT-KO	1982:1988	arg1	mice					1990:1993	GalT-KO mice	1982:1993	GalT-KO mice	1982:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	4	34	theme	16S	773:775	arg1	sequencing					761:770	metagenetic high-throughput sequencing	733:770	metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5)	733:810	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	34	theme	16S	773:775	arg1	gene					782:785	16S rRNA gene	773:785	16S rRNA gene	773:785	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	13	35	theme	microbiota	2220:2229	arg1	diversity					2231:2239	gut microbiota diversity	2216:2239	gut microbiota diversity	2216:2239	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	2	36	from	Alterations	230:240	arg1	composition					270:280	the intestinal microbial composition	245:280	the intestinal microbial composition	245:280	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	4	37	theme	life	725:728	arg1	months					708:713	7 months	706:713	7 months of animal life	706:728	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	3	38	theme	gut	430:432	arg1	microbiota					434:443	commensal gut microbiota	420:443	commensal gut microbiota	420:443	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	13	39	theme	natural	2292:2298	arg1	antibodies					2312:2321	natural anti-glycan antibodies	2292:2321	natural anti-glycan antibodies	2292:2321	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	4	40	theme	metagenetic	733:743	arg1	sequencing					761:770	metagenetic high-throughput sequencing	733:770	metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5)	733:810	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	40	theme	metagenetic	733:743	arg1	gene					782:785	16S rRNA gene	773:785	16S rRNA gene	773:785	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	13	41	theme	antibodies	2312:2321	arg1	levels					2282:2287	levels	2282:2287	levels	2282:2287	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	13	41	theme	antibodies	2312:2321	arg1	repertoire					2267:2276	different repertoire	2257:2276	different repertoire	2257:2276	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	13	41	theme	antibodies	2312:2321	arg1	antibodies					2312:2321	natural anti-glycan antibodies	2292:2321	natural anti-glycan antibodies	2292:2321	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	5	42	theme	microbial	941:949	arg1	diversity					951:959	the microbial diversity	937:959	the microbial diversity for each animal by metagenome-wide association studies (MWAS)	937:1021	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	12	43	theme	continuous	2098:2107	arg1	stimulation					2119:2129	the continuous antigenic stimulation	2094:2129	the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse	2094:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	8	44	theme	anti-glycan	1551:1561	arg1	antibodies					1563:1572	specific anti-glycan antibodies	1542:1572	specific anti-glycan antibodies	1542:1572	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	13	45	from	differences	2201:2211	arg1	diversity					2231:2239	gut microbiota diversity	2216:2239	gut microbiota diversity	2216:2239	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	11	46	theme	gut	1964:1966	arg1	microbiome					1968:1977	the gut microbiome	1960:1977	the gut microbiome of GalT-KO mice	1960:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	1	47	theme	significant	155:165	arg1	role					167:170	a significant role	153:170	a significant role	153:170	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	0	48	theme	Natural	33:39	arg1	Repertoire					52:61	Glycan-Specific Natural Antibodies Repertoire	17:61	Glycan-Specific Natural Antibodies Repertoire	17:61	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	0	49	theme	GalT-KO	66:72	arg1	Mice					74:77	GalT-KO Mice	66:77	GalT-KO Mice	66:77	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	0	50	theme	Repertoire	52:61	arg1	Formation					4:12	The Formation	0:12	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice	0:77	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	1	51	theme	immune	210:215	arg1	homeostasis					217:227	the innate and adaptive immune homeostasis	186:227	the innate and adaptive immune homeostasis	186:227	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	10	52	theme	different	1767:1775	arg1	taxa					1777:1780	different taxa	1767:1780	different taxa associated with the development of specific anti-glycan antibodies	1767:1847	We also found redundancy in different taxa associated with the development of specific anti-glycan antibodies.					
30891034	5	53	theme	metagenome-wide	980:994	arg1	MWAS					1017:1020	MWAS	1017:1020	MWAS	1017:1020	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	5	53	theme	metagenome-wide	980:994	arg1	studies					1008:1014	metagenome-wide association studies	980:1014	metagenome-wide association studies (MWAS)	980:1021	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	7	54	theme	main	1257:1260	arg1	related					1321:1327	related	1321:1327	related	1321:1327	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	54	theme	main	1257:1260	arg1	changes					1262:1268	The main changes	1253:1268	The main changes in microbiota diversity (month-2 and month-3)	1253:1314	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	4	55	theme	variable	788:795	arg1	regions					797:803	variable regions V3-V5	788:809	variable regions V3-V5	788:809	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	12	56	theme	antigenic	2109:2117	arg1	stimulation					2119:2129	the continuous antigenic stimulation	2094:2129	the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse	2094:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	3	57	theme	circulating	507:517	arg1	elements					519:526	circulating elements	507:526	circulating elements of the host immune system	507:552	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	9	58	theme	natural	1661:1667	arg1	antibodies					1681:1690	natural anti-glycan antibodies	1661:1690	natural anti-glycan antibodies	1661:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	3	59	theme	system	547:552	arg1	elements					519:526	circulating elements	507:526	circulating elements of the host immune system	507:552	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	13	60	theme	gut	2216:2218	arg1	diversity					2231:2239	gut microbiota diversity	2216:2239	gut microbiota diversity	2216:2239	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	12	61	theme	natural	2026:2032	arg1	antibodies					2052:2061	natural anti-carbohydrate antibodies	2026:2061	natural anti-carbohydrate antibodies	2026:2061	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	9	62	theme	antibodies	1681:1690	arg1	microbiota					1614:1623	the gut microbiota	1606:1623	the gut microbiota	1606:1623	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	9	62	theme	antibodies	1681:1690	arg1	antibodies					1681:1690	natural anti-glycan antibodies	1661:1690	natural anti-glycan antibodies	1661:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	9	62	theme	antibodies	1681:1690	arg1	repertoire					1647:1656	the corresponding repertoire	1629:1656	the corresponding repertoire of natural anti-glycan antibodies	1629:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	13	63	theme	anti-glycan	2300:2310	arg1	antibodies					2312:2321	natural anti-glycan antibodies	2292:2321	natural anti-glycan antibodies	2292:2321	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	1	64	contain	have	148:151	arg1	bacteria					126:133	Gut commensal bacteria	112:133	Gut commensal bacteria	112:133	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	1	64	contain	have	148:151	arg2	role					167:170	a significant role	153:170	a significant role	153:170	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	3	65	theme	host	535:538	arg1	system					547:552	the host immune system	531:552	the host immune system	531:552	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	5	66	theme	natural	847:853	arg1	antibodies					855:864	glycan-specific natural antibodies	831:864	glycan-specific natural antibodies	831:864	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	12	67	theme	antibodies	2052:2061	arg1	antibodies					2052:2061	natural anti-carbohydrate antibodies	2026:2061	natural anti-carbohydrate antibodies	2026:2061	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	12	67	theme	antibodies	2052:2061	arg1	repertoire					2012:2021	the repertoire	2008:2021	the repertoire of natural anti-carbohydrate antibodies	2008:2061	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	12	68	theme	GalT-KO	2180:2186	arg1	mouse					2188:2192	each GalT-KO mouse	2175:2192	each GalT-KO mouse	2175:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	6	69	theme	GalT-KO	1239:1245	arg1	mice					1247:1250	GalT-KO mice	1239:1250	GalT-KO mice	1239:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	10	70	theme	specific	1817:1824	arg1	antibodies					1838:1847	specific anti-glycan antibodies	1817:1847	specific anti-glycan antibodies	1817:1847	We also found redundancy in different taxa associated with the development of specific anti-glycan antibodies.					
30891034	4	71	dep	gene	782:785	arg1	regions					797:803	variable regions V3-V5	788:809	variable regions V3-V5	788:809	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	8	72	theme	gut	1508:1510	arg1	microbiota					1512:1521	the gut microbiota	1504:1521	the gut microbiota	1504:1521	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	11	73	from	Differences	1850:1860	arg1	diversity					1875:1883	microbial diversity	1865:1883	microbial diversity	1865:1883	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	7	74	from	changes	1262:1268	arg1	diversity					1284:1292	microbiota diversity	1273:1292	microbiota diversity (month-2 and month-3)	1273:1314	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	75	theme	antibodies	1398:1407	arg1	levels					1353:1358	levels	1353:1358	levels	1353:1358	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	75	theme	antibodies	1398:1407	arg1	antibodies					1398:1407	natural anti-glycan antibodies	1378:1407	natural anti-glycan antibodies	1378:1407	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	75	theme	antibodies	1398:1407	arg1	repertoire					1364:1373	repertoire	1364:1373	repertoire of natural anti-glycan antibodies in these mice	1364:1421	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	4	76	theme	gut	661:663	arg1	microbiota					665:674	the gut microbiota	657:674	the gut microbiota of inbred GalT-KO mice	657:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	2	77	theme	several	308:314	arg1	conditions					370:379	autoimmune and inflammatory conditions	342:379	autoimmune and inflammatory conditions	342:379	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	2	77	theme	several	308:314	arg1	states					324:329	several disease states	308:329	several disease states	308:329	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	7	78	theme	natural	1378:1384	arg1	antibodies					1398:1407	natural anti-glycan antibodies	1378:1407	natural anti-glycan antibodies	1378:1407	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	12	79	theme	bacterial	2151:2159	arg1	population					2161:2170	the gut bacterial population	2143:2170	the gut bacterial population of each GalT-KO mouse	2143:2192	In summary, the repertoire of natural anti-carbohydrate antibodies may be partially determined by the continuous antigenic stimulation produced by the gut bacterial population of each GalT-KO mouse.					
30891034	4	80	from	abundance	625:633	arg1	microbiota					665:674	the gut microbiota	657:674	the gut microbiota of inbred GalT-KO mice	657:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	13	81	theme	potential	2402:2410	arg1	threats					2412:2418	other potential threats	2396:2418	other potential threats	2396:2418	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	8	82	theme	positive	1450:1457	arg1	associations					1472:1483	significant positive and negative associations	1438:1483	significant positive and negative associations	1438:1483	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	6	83	theme	anti-glycan	1213:1223	arg1	antibodies					1225:1234	natural anti-glycan antibodies	1205:1234	natural anti-glycan antibodies	1205:1234	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	4	84	theme	specific	638:645	arg1	taxons					647:652	specific taxons	638:652	specific taxons	638:652	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	13	85	theme	Small	2195:2199	arg1	differences					2201:2211	Small differences	2195:2211	Small differences in gut microbiota diversity	2195:2239	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	10	86	theme	antibodies	1838:1847	arg1	development					1802:1812	the development	1798:1812	the development of specific anti-glycan antibodies	1798:1847	We also found redundancy in different taxa associated with the development of specific anti-glycan antibodies.					
30891034	1	87	theme	Gut	112:114	arg1	bacteria					126:133	Gut commensal bacteria	112:133	Gut commensal bacteria	112:133	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	8	88	theme	negative	1463:1470	arg1	associations					1472:1483	significant positive and negative associations	1438:1483	significant positive and negative associations	1438:1483	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	11	89	theme	microbial	1865:1873	arg1	diversity					1875:1883	microbial diversity	1865:1883	microbial diversity	1865:1883	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	4	90	dep	diversity	611:619	arg1	the					607:609	the	607:609	the	607:609	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	2	91	theme	microbial	260:268	arg1	composition					270:280	the intestinal microbial composition	245:280	the intestinal microbial composition	245:280	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	6	92	theme	final	1185:1189	arg1	repertoire					1191:1200	the final repertoire	1181:1200	the final repertoire of natural anti-glycan antibodies in GalT-KO mice	1181:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	6	92	theme	final	1185:1189	arg1	antibodies					1225:1234	natural anti-glycan antibodies	1205:1234	natural anti-glycan antibodies	1205:1234	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	5	93	theme	glycan	887:892	arg1	technology					900:909	printed glycan array technology	879:909	printed glycan array technology	879:909	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	0	94	from	Formation	4:12	arg1	Mice					74:77	GalT-KO Mice	66:77	GalT-KO Mice	66:77	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	4	95	theme	GalT-KO	686:692	arg1	mice					694:697	inbred GalT-KO mice	679:697	inbred GalT-KO mice	679:697	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	6	96	from	repertoire	1191:1200	arg1	mice					1247:1250	GalT-KO mice	1239:1250	GalT-KO mice	1239:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	0	97	theme	Gut	96:98	arg1	Microbiota					100:109	Gut Microbiota	96:109	Gut Microbiota	96:109	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	7	98	dep	diversity	1284:1292	arg1	month-3					1307:1313	month-3	1307:1313	month-3	1307:1313	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	98	dep	diversity	1284:1292	arg1	month-2					1295:1301	month-2	1295:1301	month-2	1295:1301	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	4	99	theme	high-throughput	745:759	arg1	sequencing					761:770	metagenetic high-throughput sequencing	733:770	metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5)	733:810	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	99	theme	high-throughput	745:759	arg1	gene					782:785	16S rRNA gene	773:785	16S rRNA gene	773:785	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	13	100	theme	other	2396:2400	arg1	threats					2412:2418	other potential threats	2396:2418	other potential threats	2396:2418	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	6	101	from	development	1166:1176	arg1	mice					1247:1250	GalT-KO mice	1239:1250	GalT-KO mice	1239:1250	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	7	102	from	changes	1342:1348	arg1	levels					1353:1358	levels	1353:1358	levels	1353:1358	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	102	from	changes	1342:1348	arg1	antibodies					1398:1407	natural anti-glycan antibodies	1378:1407	natural anti-glycan antibodies	1378:1407	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	102	from	changes	1342:1348	arg1	repertoire					1364:1373	repertoire	1364:1373	repertoire of natural anti-glycan antibodies in these mice	1364:1421	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	6	103	theme	orders	1047:1052	arg1	clostridiales					1054:1066	the orders clostridiales	1043:1066	the orders clostridiales (most abundant)	1043:1082	Our data show that the orders clostridiales (most abundant), bacteriodales, lactobacillales, and deferribacterales may be associated with the development of the final repertoire of natural anti-glycan antibodies in GalT-KO mice.					
30891034	8	104	theme	specific	1542:1549	arg1	antibodies					1563:1572	specific anti-glycan antibodies	1542:1572	specific anti-glycan antibodies	1542:1572	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	4	105	theme	animal	718:723	arg1	life					725:728	animal life	718:728	animal life	718:728	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	0	106	theme	Glycan-Specific	17:31	arg1	Repertoire					52:61	Glycan-Specific Natural Antibodies Repertoire	17:61	Glycan-Specific Natural Antibodies Repertoire	17:61	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	9	107	theme	corresponding	1633:1645	arg1	antibodies					1681:1690	natural anti-glycan antibodies	1661:1690	natural anti-glycan antibodies	1661:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	9	107	theme	corresponding	1633:1645	arg1	repertoire					1647:1656	the corresponding repertoire	1629:1656	the corresponding repertoire of natural anti-glycan antibodies	1629:1690	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	9	108	theme	individual	1585:1594	arg1	features					1596:1603	individual features	1585:1603	individual features	1585:1603	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	7	109	from	levels	1353:1358	arg1	mice					1418:1421	these mice	1412:1421	these mice	1412:1421	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	8	110	theme	antibodies	1563:1572	arg1	microbiota					1512:1521	the gut microbiota	1504:1521	the gut microbiota	1504:1521	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	8	110	theme	antibodies	1563:1572	arg1	pattern					1531:1537	the pattern	1527:1537	the pattern of specific anti-glycan antibodies	1527:1572	Additionally, significant positive and negative associations were found between the gut microbiota and the pattern of specific anti-glycan antibodies.					
30891034	3	111	theme	systemic	476:483	arg1	immunity					485:492	the systemic immunity	472:492	the systemic immunity	472:492	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	0	112	theme	Antibodies	41:50	arg1	Repertoire					52:61	Glycan-Specific Natural Antibodies Repertoire	17:61	Glycan-Specific Natural Antibodies Repertoire	17:61	The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice Is Determined by Gut Microbiota.					
30891034	7	113	from	repertoire	1364:1373	arg1	mice					1418:1421	these mice	1412:1421	these mice	1412:1421	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	7	114	theme	microbiota	1273:1282	arg1	diversity					1284:1292	microbiota diversity	1273:1292	microbiota diversity (month-2 and month-3)	1273:1314	The main changes in microbiota diversity (month-2 and month-3) were related to important changes in levels and repertoire of natural anti-glycan antibodies in these mice.					
30891034	3	115	theme	commensal	420:428	arg1	microbiota					434:443	commensal gut microbiota	420:443	commensal gut microbiota	420:443	However, it is not entirely clear how commensal gut microbiota modulate and contribute to the systemic immunity, and whether circulating elements of the host immune system could regulate the microbiome.					
30891034	11	116	theme	microbiome	1968:1977	arg1	output					1950:1955	the overall functional output	1927:1955	the overall functional output of the gut microbiome of GalT-KO mice	1927:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	9	117	theme	gut	1610:1612	arg1	microbiota					1614:1623	the gut microbiota	1606:1623	the gut microbiota	1606:1623	Regarding individual features, the gut microbiota and the corresponding repertoire of natural anti-glycan antibodies showed differences among the examined animals.					
30891034	2	118	theme	autoimmune	342:351	arg1	conditions					370:379	autoimmune and inflammatory conditions	342:379	autoimmune and inflammatory conditions	342:379	Alterations in the intestinal microbial composition have been associated with several disease states, including autoimmune and inflammatory conditions.					
30891034	4	119	theme	rRNA	777:780	arg1	sequencing					761:770	metagenetic high-throughput sequencing	733:770	metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5)	733:810	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	4	119	theme	rRNA	777:780	arg1	gene					782:785	16S rRNA gene	773:785	16S rRNA gene	773:785	Thus, we have studied the diversity and abundance of specific taxons in the gut microbiota of inbred GalT-KO mice during 7 months of animal life by metagenetic high-throughput sequencing (16S rRNA gene, variable regions V3-V5).					
30891034	1	120	theme	adaptive	201:208	arg1	homeostasis					217:227	the innate and adaptive immune homeostasis	186:227	the innate and adaptive immune homeostasis	186:227	Gut commensal bacteria are known to have a significant role in regulating the innate and adaptive immune homeostasis.					
30891034	5	121	theme	association	996:1006	arg1	MWAS					1017:1020	MWAS	1017:1020	MWAS	1017:1020	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	5	121	theme	association	996:1006	arg1	studies					1008:1014	metagenome-wide association studies	980:1014	metagenome-wide association studies (MWAS)	980:1021	The repertoire of glycan-specific natural antibodies, obtained by printed glycan array technology, was then associated with the microbial diversity for each animal by metagenome-wide association studies (MWAS).					
30891034	11	122	theme	overall	1931:1937	arg1	output					1950:1955	the overall functional output	1927:1955	the overall functional output of the gut microbiome of GalT-KO mice	1927:1993	Differences in microbial diversity did not, therefore, necessarily influence the overall functional output of the gut microbiome of GalT-KO mice.					
30891034	13	123	theme	different	2257:2265	arg1	repertoire					2267:2276	different repertoire	2257:2276	different repertoire	2257:2276	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
30891034	13	123	theme	different	2257:2265	arg1	antibodies					2312:2321	natural anti-glycan antibodies	2292:2321	natural anti-glycan antibodies	2292:2321	Small differences in gut microbiota diversity could determine different repertoire and levels of natural anti-glycan antibodies and consequently might induce different immune responses to pathogens or other potential threats.					
31261000	0	0	theme	cellulose	87:95	arg1	nanofiber					97:105	cellulose nanofiber	87:105	cellulose nanofiber	87:105	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	5	1	theme	1.79-5.38	769:777	arg1	%					778:778	%	778:778	%	778:778	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	4	2	theme	thermal	688:694	arg1	stability					696:704	thermal stability	688:704	thermal stability	688:704	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	4	3	theme	hemicelluloses-chitosan	548:570	arg1	matrix					572:577	hemicelluloses-chitosan matrix	548:577	hemicelluloses-chitosan matrix	548:577	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	5	4	theme	%	778:778	arg1	XOS					780:782	1.79-5.38% XOS	769:782	1.79-5.38% XOS	769:782	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	5	theme	7.18	900:903	arg1	XOS					906:908	7.18% XOS	900:908	7.18% XOS	900:908	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	6	6	theme	simulated	964:972	arg1	fluid					982:986	simulated gastric fluid	964:986	simulated gastric fluid	964:986	Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min.					
31261000	0	7	from	Use	0:2	arg1	films					67:71	hemicelluloses/chitosan-based films	37:71	hemicelluloses/chitosan-based films	37:71	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	7	8	theme	high	1167:1170	arg1	TS					1172:1173	high TS	1167:1173	high TS	1167:1173	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	4	9	theme	vapor	613:617	arg1	permeability					619:630	slightly higher water vapor permeability	591:630	slightly higher water vapor permeability	591:630	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	4	10	theme	high	711:714	arg1	transparency					716:727	high transparency	711:727	high transparency	711:727	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	1	11	theme	Edible	146:151	arg1	films					163:167	Edible packaging films	146:167	Edible packaging films	146:167	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	5	12	theme	oxygen	824:829	arg1	permeability					831:842	lower oxygen permeability	818:842	lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa)	818:876	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	3	13	theme	film	456:459	arg1	TS					445:446	TS	445:446	TS	445:446	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	3	13	theme	film	456:459	arg1	strength					435:442	the tensile strength	423:442	the tensile strength (TS) of the film	423:459	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	3	14	theme	5 wt	503:506	arg1	CNF					509:511	5 wt% CNF	503:511	5 wt% CNF	503:511	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	7	15	with	XOS	1012:1014	arg1	properties					1044:1053	prebiotic properties	1034:1053	prebiotic properties can be added to films successfully to improve the functionality	1034:1117	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	1	16	theme	important	177:185	arg1	role					187:190	an important role	174:190	an important role	174:190	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	5	17	theme	hemicelluloses/chitosan	734:756	arg1	films					758:762	The hemicelluloses/chitosan films	730:762	The hemicelluloses/chitosan films with 1.79-5.38% XOS	730:782	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	6	18	theme	gastric	974:980	arg1	fluid					982:986	simulated gastric fluid	964:986	simulated gastric fluid	964:986	Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min.					
31261000	3	19	theme	%	507:507	arg1	CNF					509:511	5 wt% CNF	503:511	5 wt% CNF	503:511	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	7	20	theme	prebiotic	1034:1042	arg1	properties					1044:1053	prebiotic properties	1034:1053	prebiotic properties can be added to films successfully to improve the functionality	1034:1117	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	2	21	theme	reinforced	296:305	arg1	films					344:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films	270:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS)	270:380	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	3	22	theme	tensile	427:433	arg1	TS					445:446	TS	445:446	TS	445:446	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	3	22	theme	tensile	427:433	arg1	strength					435:442	the tensile strength	423:442	the tensile strength (TS) of the film	423:459	Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF.					
31261000	4	23	theme	1.79-7.18	528:536	arg1	%					537:537	%	537:537	%	537:537	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	5	24	dep	higher	788:793	arg1	permeability					831:842	lower oxygen permeability	818:842	lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa)	818:876	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	24	dep	higher	788:793	arg1	42.7-50.7 MPa					799:811	42.7-50.7 MPa	799:811	42.7-50.7 MPa	799:811	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	24	dep	higher	788:793	arg1	TS					795:796	TS	795:796	TS (42.7-50.7 MPa)	795:812	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	7	25	with	films	1127:1131	arg1	properties					1044:1053	prebiotic properties	1034:1053	prebiotic properties can be added to films successfully to improve the functionality	1034:1117	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	5	26	theme	lower	818:822	arg1	permeability					831:842	lower oxygen permeability	818:842	lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa)	818:876	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	0	27	theme	xylooligosaccharides	7:26	arg1	Use					0:2	Use	0:2	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films	0:71	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	0	28	theme	physicochemical	118:132	arg1	properties					134:143	physicochemical properties	118:143	physicochemical properties	118:143	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	1	29	theme	shelf	209:213	arg1	life					215:218	the shelf life	205:218	the shelf life of food products	205:235	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	4	30	theme	%	537:537	arg1	XOS					539:541	1.79-7.18% XOS	528:541	1.79-7.18% XOS	528:541	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	4	31	theme	composite	641:649	arg1	films					651:655	the composite films	637:655	the composite films	637:655	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	7	32	dep	properties	1044:1053	arg1	added					1062:1066	added	1062:1066	can be added to films successfully to improve the functionality	1055:1117	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	0	33	theme	hemicelluloses/chitosan-based	37:65	arg1	films					67:71	hemicelluloses/chitosan-based films	37:71	hemicelluloses/chitosan-based films	37:71	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	6	34	theme	%	930:930	arg1	XOS					932:934	∼92.6% XOS	925:934	∼92.6% XOS in films	925:943	Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min.					
31261000	2	35	with	films	344:348	arg1	XOS					377:379	XOS	377:379	XOS	377:379	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	2	35	with	films	344:348	arg1	xylooligosaccharides					355:374	xylooligosaccharides	355:374	xylooligosaccharides (XOS)	355:380	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	5	36	theme	%	904:904	arg1	XOS					906:908	7.18% XOS	900:908	7.18% XOS	900:908	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	6	37	theme	∼92.6	925:929	arg1	%					930:930	%	930:930	%	930:930	Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min.					
31261000	2	38	theme	films	344:348	arg1	properties					256:265	the properties	252:265	the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS)	252:380	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	6	39	from	XOS	932:934	arg1	films					939:943	films	939:943	films	939:943	Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min.					
31261000	0	40	from	Effect	108:113	arg1	properties					134:143	physicochemical properties	118:143	physicochemical properties	118:143	Use of xylooligosaccharides (XOS) in hemicelluloses/chitosan-based films reinforced by cellulose nanofiber: Effect on physicochemical properties.					
31261000	5	41	dep	permeability	831:842	arg1	4.95-5.06 cm3 μm/m2·day·kPa					849:875	4.95-5.06 cm3 μm/m2·day·kPa	849:875	4.95-5.06 cm3 μm/m2·day·kPa	849:875	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	41	dep	permeability	831:842	arg1	OP					845:846	OP	845:846	OP	845:846	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	2	42	theme	edible	337:342	arg1	films					344:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films	270:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS)	270:380	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	1	43	theme	food	223:226	arg1	products					228:235	food products	223:235	food products	223:235	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	2	44	theme	hemicelluloses/chitosan-based	307:335	arg1	films					344:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films	270:348	cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS)	270:380	In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated.					
31261000	1	45	theme	products	228:235	arg1	life					215:218	the shelf life	205:218	the shelf life of food products	205:235	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	5	46	with	films	758:762	arg1	XOS					780:782	1.79-5.38% XOS	769:782	1.79-5.38% XOS	769:782	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	47	contain	containing	889:898	arg2	XOS					906:908	7.18% XOS	900:908	7.18% XOS	900:908	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	5	47	contain	containing	889:898	arg1	those					883:887	those	883:887	those	883:887	The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 μm/m2·day·kPa) than those containing 7.18% XOS.					
31261000	1	48	theme	packaging	153:161	arg1	films					163:167	Edible packaging films	146:167	Edible packaging films	146:167	Edible packaging films play an important role in extending the shelf life of food products.					
31261000	4	49	theme	water	607:611	arg1	permeability					619:630	slightly higher water vapor permeability	591:630	slightly higher water vapor permeability	591:630	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	7	50	theme	low	1179:1181	arg1	OP					1183:1184	low OP	1179:1184	low OP	1179:1184	Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.					
31261000	4	51	theme	good	667:670	arg1	hydrophobicity					672:685	good hydrophobicity	667:685	good hydrophobicity	667:685	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
31261000	4	52	theme	higher	600:605	arg1	permeability					619:630	slightly higher water vapor permeability	591:630	slightly higher water vapor permeability	591:630	Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency.					
30640029	4	0	theme	equal	752:756	arg1	activity					776:783	equal or even increased activity	752:783	equal or even increased activity	752:783	When combined with an LPMO, several less processive chitinase mutants showed equal or even increased activity on chitin compared to the wild-type.					
30640029	1	1	theme	recalcitrant	197:208	arg1	cellulose					234:242	cellulose	234:242	cellulose	234:242	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	1	1	theme	recalcitrant	197:208	arg1	chitin					248:253	chitin	248:253	chitin	248:253	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	1	1	theme	recalcitrant	197:208	arg1	polysaccharides					210:224	recalcitrant polysaccharides	197:224	recalcitrant polysaccharides such as cellulose and chitin	197:253	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	2	2	from	degradation	399:409	arg1	efficient					382:390	efficient	382:390	efficient	382:390	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	2	2	from	degradation	399:409	arg1	mechanism					369:377	The processive mechanism	354:377	The processive mechanism	354:377	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	2	3	theme	substrates	424:433	arg1	degradation					399:409	the degradation	395:409	the degradation of insoluble substrates	395:433	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	0	4	theme	glycoside	116:124	arg1	processivity					136:147	glycoside hydrolase processivity	116:147	glycoside hydrolase processivity	116:147	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	2	5	theme	processive	358:367	arg1	efficient					382:390	efficient	382:390	efficient	382:390	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	2	5	theme	processive	358:367	arg1	mechanism					369:377	The processive mechanism	354:377	The processive mechanism	354:377	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	2	6	from	efficient	382:390	arg1	degradation					399:409	the degradation	395:409	the degradation of insoluble substrates	395:433	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	5	7	from	processivity	854:865	arg1	cocktails					902:910	polysaccharide degrading enzyme cocktails	870:910	polysaccharide degrading enzyme cocktails	870:910	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	3	8	theme	crystalline	563:573	arg1	chitin					575:580	crystalline chitin	563:580	crystalline chitin	563:580	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	1	9	theme	crystalline	333:343	arg1	surface					345:351	the crystalline surface	329:351	the crystalline surface	329:351	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	5	10	theme	enzyme	895:900	arg1	cocktails					902:910	polysaccharide degrading enzyme cocktails	870:910	polysaccharide degrading enzyme cocktails	870:910	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	5	11	theme	cocktails	956:964	arg1	composition					936:946	the composition	932:946	the composition of such cocktails	932:964	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	5	12	theme	degrading	885:893	arg1	cocktails					902:910	polysaccharide degrading enzyme cocktails	870:910	polysaccharide degrading enzyme cocktails	870:910	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	4	13	theme	several	703:709	arg1	mutants					737:743	several less processive chitinase mutants	703:743	several less processive chitinase mutants	703:743	When combined with an LPMO, several less processive chitinase mutants showed equal or even increased activity on chitin compared to the wild-type.					
30640029	0	14	theme	hydrolase	126:134	arg1	processivity					136:147	glycoside hydrolase processivity	116:147	glycoside hydrolase processivity	116:147	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	4	15	theme	processive	716:725	arg1	mutants					737:743	several less processive chitinase mutants	703:743	several less processive chitinase mutants	703:743	When combined with an LPMO, several less processive chitinase mutants showed equal or even increased activity on chitin compared to the wild-type.					
30640029	0	16	theme	crystalline	26:36	arg1	polysaccharides					38:52	recalcitrant crystalline polysaccharides	13:52	recalcitrant crystalline polysaccharides	13:52	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	5	17	theme	polysaccharide	870:883	arg1	cocktails					902:910	polysaccharide degrading enzyme cocktails	870:910	polysaccharide degrading enzyme cocktails	870:910	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	0	18	theme	recalcitrant	13:24	arg1	polysaccharides					38:52	recalcitrant crystalline polysaccharides	13:52	recalcitrant crystalline polysaccharides	13:52	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	5	19	theme	such	951:954	arg1	cocktails					956:964	such cocktails	951:964	such cocktails	951:964	Thus, LPMOs affect the need for processivity in polysaccharide degrading enzyme cocktails, which implies that the composition of such cocktails may need reconsideration.					
30640029	2	20	theme	speed	476:480	arg1	cost					453:456	the cost	449:456	the cost of reduced enzyme speed	449:480	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	3	21	theme	processive	501:510	arg1	variants					522:529	less processive chitinase variants	496:529	less processive chitinase variants	496:529	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	2	22	theme	enzyme	469:474	arg1	speed					476:480	reduced enzyme speed	461:480	reduced enzyme speed	461:480	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	0	23	theme	polysaccharides	38:52	arg1	Treatment					0:8	Treatment	0:8	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase	0:92	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	2	24	theme	reduced	461:467	arg1	speed					476:480	reduced enzyme speed	461:480	reduced enzyme speed	461:480	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	0	25	theme	polysaccharide	65:78	arg1	monooxygenase					80:92	lytic polysaccharide monooxygenase	59:92	lytic polysaccharide monooxygenase	59:92	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	3	26	theme	lytic	632:636	arg1	monooxygenase					653:665	a lytic polysaccharide monooxygenase	630:665	a lytic polysaccharide monooxygenase (LPMO)	630:672	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	3	26	theme	lytic	632:636	arg1	LPMO					668:671	LPMO	668:671	LPMO	668:671	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	2	27	theme	insoluble	414:422	arg1	substrates					424:433	insoluble substrates	414:433	insoluble substrates	414:433	The processive mechanism is efficient in the degradation of insoluble substrates, but comes at the cost of reduced enzyme speed.					
30640029	0	28	theme	lytic	59:63	arg1	monooxygenase					80:92	lytic polysaccharide monooxygenase	59:92	lytic polysaccharide monooxygenase	59:92	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	3	29	theme	polysaccharide	638:651	arg1	monooxygenase					653:665	a lytic polysaccharide monooxygenase	630:665	a lytic polysaccharide monooxygenase (LPMO)	630:672	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	3	29	theme	polysaccharide	638:651	arg1	LPMO					668:671	LPMO	668:671	LPMO	668:671	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	1	30	theme	Processive	150:159	arg1	hydrolases					171:180	Processive glycoside hydrolases	150:180	Processive glycoside hydrolases	150:180	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	0	31	with	Treatment	0:8	arg1	monooxygenase					80:92	lytic polysaccharide monooxygenase	59:92	lytic polysaccharide monooxygenase	59:92	Treatment of recalcitrant crystalline polysaccharides with lytic polysaccharide monooxygenase relieves the need for glycoside hydrolase processivity.					
30640029	1	32	theme	glycoside	161:169	arg1	hydrolases					171:180	Processive glycoside hydrolases	150:180	Processive glycoside hydrolases	150:180	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	1	33	theme	glycosidic	277:286	arg1	linkages					288:295	glycosidic linkages	277:295	glycosidic linkages	277:295	Processive glycoside hydrolases associate with recalcitrant polysaccharides such as cellulose and chitin and repeatedly cleave glycosidic linkages without fully dissociating from the crystalline surface.					
30640029	4	34	theme	increased	766:774	arg1	activity					776:783	equal or even increased activity	752:783	equal or even increased activity	752:783	When combined with an LPMO, several less processive chitinase mutants showed equal or even increased activity on chitin compared to the wild-type.					
30640029	3	35	theme	reduced	536:542	arg1	ability					544:550	reduced ability	536:550	reduced ability	536:550	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	3	36	theme	chitinase	512:520	arg1	variants					522:529	less processive chitinase variants	496:529	less processive chitinase variants	496:529	We show that less processive chitinase variants with reduced ability to degrade crystalline chitin, regain much of this ability when combined with a lytic polysaccharide monooxygenase (LPMO).					
30640029	4	37	theme	chitinase	727:735	arg1	mutants					737:743	several less processive chitinase mutants	703:743	several less processive chitinase mutants	703:743	When combined with an LPMO, several less processive chitinase mutants showed equal or even increased activity on chitin compared to the wild-type.					
30471396	2	0	theme	extraction	660:669	arg1	method					671:676	ultrasound extraction method	649:676	ultrasound extraction method (3.6%)	649:683	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	2	0	theme	extraction	660:669	arg1	%					682:682	3.6%	679:682	3.6%	679:682	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	6	1	theme	microwave-	1132:1141	arg1	fucoidans					1175:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	1	2	theme	extraction	278:287	arg1	procedures					289:298	conventional and non-conventional extraction procedures	244:298	conventional and non-conventional extraction procedures	244:298	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	0	3	theme	antiviral	81:89	arg1	activity					91:98	the antibacterial and antiviral activity	59:98	the antibacterial and antiviral activity of fucoidans	59:111	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	1	4	theme	saccharide	395:404	arg1	composition					406:416	saccharide composition	395:416	saccharide composition	395:416	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	2	5	theme	ultrasound	649:658	arg1	method					671:676	ultrasound extraction method	649:676	ultrasound extraction method (3.6%)	649:683	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	2	5	theme	ultrasound	649:658	arg1	%					682:682	3.6%	679:682	3.6%	679:682	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	5	6	theme	FT-IR	938:942	arg1	spectroscopy					944:955	The FT-IR spectroscopy	934:955	The FT-IR spectroscopy	934:955	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	0	7	theme	fucoidans	103:111	arg1	activity					91:98	the antibacterial and antiviral activity	59:98	the antibacterial and antiviral activity of fucoidans	59:111	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	0	8	from	Effect	0:5	arg1	activity					91:98	the antibacterial and antiviral activity	59:98	the antibacterial and antiviral activity of fucoidans	59:111	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	5	9	theme	bending	1001:1007	arg1	vibration					1009:1017	bending vibration	1001:1017	bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1001:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	10	from	vibration	1009:1017	arg1	1250 cm-1					1074:1082	1250 cm-1	1074:1082	1250 cm-1	1074:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	10	from	vibration	1009:1017	arg1	818					1066:1068	818	1066:1068	818	1066:1068	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	6	11	theme	water-extracted	1159:1173	arg1	fucoidans					1175:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	7	12	theme	EC50	1494:1497	arg1	values					1499:1504	EC50 values	1494:1504	EC50 values in the 0.027-0.123 μg/mL range	1494:1535	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	7	13	theme	0.027-0.123 μg/mL	1513:1529	arg1	range					1531:1535	the 0.027-0.123 μg/mL range	1509:1535	the 0.027-0.123 μg/mL range	1509:1535	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	6	14	theme	Antibacterial	1099:1111	arg1	assays					1113:1118	Antibacterial assays	1099:1118	Antibacterial assays	1099:1118	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	2	15	theme	fucoidans	581:589	arg1	yield					591:595	the highest fucoidans yield	569:595	the highest fucoidans yield (13.15%)	569:604	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	2	15	theme	fucoidans	581:589	arg1	%					603:603	13.15%	598:603	13.15%	598:603	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	1	16	theme	extracts	425:432	arg1	characteristics					375:389	biochemical characteristics	363:389	biochemical characteristics	363:389	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	1	16	theme	extracts	425:432	arg1	composition					406:416	saccharide composition	395:416	saccharide composition	395:416	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	2	17	theme	water	545:549	arg1	extraction					551:560	subcritical water extraction	533:560	subcritical water extraction	533:560	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	5	18	theme	COS	1022:1024	arg1	peaks					1057:1061	COS and stretching vibration of SO peaks	1022:1061	COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1022:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	7	19	theme	strong	1438:1443	arg1	activity					1455:1462	strong antiviral activity	1438:1462	strong antiviral activity	1438:1462	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	5	20	from	1250 cm-1	1074:1082	arg1	vibration					1009:1017	bending vibration	1001:1017	bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1001:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	20	from	1250 cm-1	1074:1082	arg1	peaks					1057:1061	COS and stretching vibration of SO peaks	1022:1061	COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1022:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	4	21	theme	fucoidans	891:899	arg1	weight					881:886	The weight mean average molecular weight	847:886	The weight mean average molecular weight of fucoidans	847:899	The weight mean average molecular weight of fucoidans varied between 444 and 1184 kDa.					
30471396	6	22	theme	inhibitory	1317:1326	arg1	effects					1328:1334	inhibitory effects	1317:1334	inhibitory effects against P. aeruginosa	1317:1356	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	1	23	theme	brown	190:194	arg1	zanardinii					214:223	brown alga Nizamuddinia zanardinii	190:223	brown alga Nizamuddinia zanardinii	190:223	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	7	24	theme	best	1598:1601	arg1	index					1615:1619	the best selectivity index	1594:1619	the best selectivity index	1594:1619	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	3	25	theme	molar	779:783	arg1	percentages					785:795	molar percentages	779:795	molar percentages	779:795	The polysaccharide chains consisted of fucose, galactose, glucose, mannose and xylose, whose molar percentages differed according to the extraction method used.					
30471396	1	26	from	effects	316:322	arg1	characteristics					375:389	biochemical characteristics	363:389	biochemical characteristics	363:389	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	1	26	from	effects	316:322	arg1	composition					406:416	saccharide composition	395:416	saccharide composition	395:416	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	5	27	theme	sulfate	983:989	arg1	esters					991:996	sulfate esters	983:996	sulfate esters	983:996	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	7	28	theme	HSV-2	1472:1476	arg1	infection					1478:1486	HSV-2 infection	1472:1486	HSV-2 infection	1472:1486	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	1	29	theme	alga	196:199	arg1	zanardinii					214:223	brown alga Nizamuddinia zanardinii	190:223	brown alga Nizamuddinia zanardinii	190:223	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	0	30	theme	non-conventional	20:35	arg1	methods					48:54	different non-conventional extraction methods	10:54	different non-conventional extraction methods	10:54	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	6	31	theme	subcritical	1269:1279	arg1	water					1281:1285	subcritical water	1269:1285	subcritical water	1269:1285	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	5	32	theme	esters	991:996	arg1	presence					971:978	the presence	967:978	the presence of sulfate esters	967:996	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	33	theme	SO	1054:1055	arg1	vibration					1041:1049	stretching vibration	1030:1049	stretching vibration of SO	1030:1055	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	33	theme	SO	1054:1055	arg1	COS					1022:1024	COS	1022:1024	COS	1022:1024	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	1	34	theme	Nizamuddinia	201:212	arg1	zanardinii					214:223	brown alga Nizamuddinia zanardinii	190:223	brown alga Nizamuddinia zanardinii	190:223	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	0	35	theme	different	10:18	arg1	methods					48:54	different non-conventional extraction methods	10:54	different non-conventional extraction methods	10:54	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	0	36	theme	Nizamuddinia	128:139	arg1	zanardinii					141:150	Nizamuddinia zanardinii	128:150	Nizamuddinia zanardinii	128:150	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	7	37	theme	extracted	1410:1418	arg1	fucoidans					1420:1428	all the extracted fucoidans	1402:1428	all the extracted fucoidans	1402:1428	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	6	38	theme	E.coli	1209:1214	arg1	growth					1199:1204	the growth	1195:1204	the growth of E.coli	1195:1214	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	0	39	theme	methods	48:54	arg1	Effect					0:5	Effect	0:5	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans	0:111	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	3	40	theme	polysaccharide	690:703	arg1	chains					705:710	The polysaccharide chains	686:710	The polysaccharide chains	686:710	The polysaccharide chains consisted of fucose, galactose, glucose, mannose and xylose, whose molar percentages differed according to the extraction method used.					
30471396	4	41	theme	weight	851:856	arg1	weight					881:886	The weight mean average molecular weight	847:886	The weight mean average molecular weight of fucoidans	847:899	The weight mean average molecular weight of fucoidans varied between 444 and 1184 kDa.					
30471396	0	42	theme	extraction	37:46	arg1	methods					48:54	different non-conventional extraction methods	10:54	different non-conventional extraction methods	10:54	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	1	43	theme	introduced	336:345	arg1	technologies					347:358	recently introduced technologies	327:358	recently introduced technologies	327:358	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	2	44	theme	subcritical	533:543	arg1	extraction					551:560	subcritical water extraction	533:560	subcritical water extraction	533:560	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	5	45	theme	peaks	1057:1061	arg1	vibration					1009:1017	bending vibration	1001:1017	bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1001:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	1	46	theme	technologies	347:358	arg1	effects					316:322	the effects	312:322	the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts	312:432	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	7	47	theme	antiviral	1445:1453	arg1	activity					1455:1462	strong antiviral activity	1438:1462	strong antiviral activity	1438:1462	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	1	48	theme	antibacterial	452:464	arg1	properties					491:500	their antibacterial, antiviral and cytotoxic properties	446:500	their antibacterial, antiviral and cytotoxic properties	446:500	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	4	49	theme	average	863:869	arg1	weight					881:886	The weight mean average molecular weight	847:886	The weight mean average molecular weight of fucoidans	847:899	The weight mean average molecular weight of fucoidans varied between 444 and 1184 kDa.					
30471396	7	50	theme	viscozyme-extracted	1549:1567	arg1	macromolecules					1569:1582	the viscozyme-extracted macromolecules	1545:1582	the viscozyme-extracted macromolecules	1545:1582	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	7	51	theme	Antiviral	1370:1378	arg1	studies					1380:1386	Antiviral studies	1370:1386	Antiviral studies	1370:1386	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	7	52	theme	selectivity	1603:1613	arg1	index					1615:1619	the best selectivity index	1594:1619	the best selectivity index	1594:1619	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	4	53	theme	molecular	871:879	arg1	weight					881:886	The weight mean average molecular weight	847:886	The weight mean average molecular weight of fucoidans	847:899	The weight mean average molecular weight of fucoidans varied between 444 and 1184 kDa.					
30471396	4	54	theme	mean	858:861	arg1	weight					881:886	The weight mean average molecular weight	847:886	The weight mean average molecular weight of fucoidans	847:899	The weight mean average molecular weight of fucoidans varied between 444 and 1184 kDa.					
30471396	7	55	from	values	1499:1504	arg1	range					1531:1535	the 0.027-0.123 μg/mL range	1509:1535	the 0.027-0.123 μg/mL range	1509:1535	Antiviral studies revealed that all the extracted fucoidans exerted strong antiviral activity against HSV-2 infection, with EC50 values in the 0.027-0.123 μg/mL range; indeed the viscozyme-extracted macromolecules displayed the best selectivity index.					
30471396	1	56	theme	biochemical	363:373	arg1	characteristics					375:389	biochemical characteristics	363:389	biochemical characteristics	363:389	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	2	57	theme	lowest	617:622	arg1	yield					624:628	the lowest yield	613:628	the lowest yield	613:628	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	1	58	theme	antiviral	467:475	arg1	properties					491:500	their antibacterial, antiviral and cytotoxic properties	446:500	their antibacterial, antiviral and cytotoxic properties	446:500	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	6	59	theme	extracted	1287:1295	arg1	fucoidans					1297:1305	extracted fucoidans	1287:1305	extracted fucoidans	1287:1305	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	1	60	from	zanardinii	214:223	arg1	fucoidans					175:183	fucoidans	175:183	fucoidans from brown alga Nizamuddinia zanardinii	175:223	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	5	61	theme	stretching	1030:1039	arg1	vibration					1041:1049	stretching vibration	1030:1049	stretching vibration of SO	1030:1055	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	62	from	818	1066:1068	arg1	vibration					1009:1017	bending vibration	1001:1017	bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1001:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	5	62	from	818	1066:1068	arg1	peaks					1057:1061	COS and stretching vibration of SO peaks	1022:1061	COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1022:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	1	63	theme	conventional	244:255	arg1	procedures					289:298	conventional and non-conventional extraction procedures	244:298	conventional and non-conventional extraction procedures	244:298	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	2	64	theme	highest	573:579	arg1	yield					591:595	the highest fucoidans yield	569:595	the highest fucoidans yield (13.15%)	569:604	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	2	64	theme	highest	573:579	arg1	%					603:603	13.15%	598:603	13.15%	598:603	The results demonstrated that subcritical water extraction showed the highest fucoidans yield (13.15%), while the lowest yield was obtained using ultrasound extraction method (3.6%).					
30471396	1	65	theme	cytotoxic	481:489	arg1	properties					491:500	their antibacterial, antiviral and cytotoxic properties	446:500	their antibacterial, antiviral and cytotoxic properties	446:500	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	5	66	theme	vibration	1041:1049	arg1	peaks					1057:1061	COS and stretching vibration of SO peaks	1022:1061	COS and stretching vibration of SO peaks at 818 and 1250 cm-1	1022:1082	The FT-IR spectroscopy confirmed the presence of sulfate esters by bending vibration of COS and stretching vibration of SO peaks at 818 and 1250 cm-1, respectively.					
30471396	6	67	dep	enzyme-ultrasound	1225:1241	arg1	fucoidans					1297:1305	extracted fucoidans	1287:1305	extracted fucoidans	1287:1305	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	6	68	theme	subcritical	1147:1157	arg1	fucoidans					1175:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	microwave- and subcritical water-extracted fucoidans	1132:1183	Antibacterial assays showed that microwave- and subcritical water-extracted fucoidans inhibited the growth of E.coli and that enzyme-ultrasound, ultrasound-microwave and subcritical water extracted fucoidans exhibited inhibitory effects against P. aeruginosa at 2 mg/mL.					
30471396	3	69	theme	extraction	823:832	arg1	method					834:839	the extraction method	819:839	the extraction method used	819:844	The polysaccharide chains consisted of fucose, galactose, glucose, mannose and xylose, whose molar percentages differed according to the extraction method used.					
30471396	0	70	theme	antibacterial	63:75	arg1	activity					91:98	the antibacterial and antiviral activity	59:98	the antibacterial and antiviral activity of fucoidans	59:111	Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii.					
30471396	1	71	theme	current	160:166	arg1	study					168:172	the current study	156:172	the current study	156:172	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
30471396	1	72	theme	non-conventional	261:276	arg1	procedures					289:298	conventional and non-conventional extraction procedures	244:298	conventional and non-conventional extraction procedures	244:298	In the current study, fucoidans from brown alga Nizamuddinia zanardinii were isolated with conventional and non-conventional extraction procedures to evaluate the effects of recently introduced technologies on biochemical characteristics and saccharide composition of the extracts, along with their antibacterial, antiviral and cytotoxic properties.					
31623207	0	0	from	Polysaccharides	10:24	arg1	Cells					78:82	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	Cells	78:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	0	0	from	Polysaccharides	10:24	arg1	L					50:50	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	L	50:50	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	6	1	theme	scanning	686:693	arg1	TG-DSC					708:713	TG-DSC	708:713	TG-DSC	708:713	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	6	1	theme	scanning	686:693	arg1	calorimetry					695:705	thermogravimetry-differential scanning calorimetry	656:705	thermogravimetry-differential scanning calorimetry (TG-DSC)	656:714	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	0	2	theme	Cancer	71:76	arg1	Cells					78:82	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	Cells	78:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	4	3	theme	PCp-I	472:476	arg1	structure					459:467	the part structure	450:467	the part structure of PCp-I	450:476	Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified.					
31623207	7	4	theme	cancer	840:845	arg1	cells					847:851	anti-A549 lung cancer cells	825:851	significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM)	813:889	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	1	5	with	heteroglycan	127:138	arg1	activity					192:199	anti-A549 lung cancer cells activity	164:199	anti-A549 lung cancer cells activity	164:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	7	6	dep	=	868:868	arg1	μM					887:888	64.84 and 126.30 μM	870:888	64.84 and 126.30 μM	870:888	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	7	7	theme	cells	847:851	arg1	activity					853:860	significant anti-A549 lung cancer cells activity	813:860	significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM)	813:889	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	6	8	theme	thermogravimetry-differential	656:684	arg1	TG-DSC					708:713	TG-DSC	708:713	TG-DSC	708:713	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	6	8	theme	thermogravimetry-differential	656:684	arg1	calorimetry					695:705	thermogravimetry-differential scanning calorimetry	656:705	thermogravimetry-differential scanning calorimetry (TG-DSC)	656:714	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	6	9	contain	had	745:747	arg2	stability					762:770	good thermal stability	749:770	good thermal stability	749:770	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	6	9	contain	had	745:747	arg1	PCp-II					738:743	PCp-II	738:743	PCp-II	738:743	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	6	9	contain	had	745:747	arg1	PCp-I					728:732	PCp-I	728:732	PCp-I	728:732	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	4	10	theme	NMR	432:434	arg1	spectroscopy					436:447	NMR spectroscopy	432:447	NMR spectroscopy	432:447	Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified.					
31623207	6	11	theme	thermal	754:760	arg1	stability					762:770	good thermal stability	749:770	good thermal stability	749:770	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	2	12	theme	molecular	258:266	arg1	weights					268:274	Their average molecular weights	244:274	Their average molecular weights	244:274	Their average molecular weights were 2.721 × 104 and 2.850 × 104.					
31623207	7	13	theme	lung	835:838	arg1	cells					847:851	anti-A549 lung cancer cells	825:851	significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM)	813:889	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	5	14	contain	had	562:564	arg2	structure					586:594	an irregular porous structure	566:594	an irregular porous structure	566:594	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	5	14	contain	had	562:564	arg1	PCp-I					556:560	PCp-I	556:560	PCp-I	556:560	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	5	15	theme	irregular	569:577	arg1	structure					586:594	an irregular porous structure	566:594	an irregular porous structure	566:594	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	5	16	theme	microscope	527:536	arg1	results					498:504	The results	494:504	The results of scanning electron microscope (SEM)	494:542	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	2	17	theme	average	250:256	arg1	weights					268:274	Their average molecular weights	244:274	Their average molecular weights	244:274	Their average molecular weights were 2.721 × 104 and 2.850 × 104.					
31623207	3	18	theme	monosaccharide	340:353	arg1	composition					355:365	the same monosaccharide composition	331:365	the same monosaccharide composition	331:365	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	5	19	theme	porous	579:584	arg1	structure					586:594	an irregular porous structure	566:594	an irregular porous structure	566:594	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	7	20	theme	significant	813:823	arg1	activity					853:860	significant anti-A549 lung cancer cells activity	813:860	significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM)	813:889	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	7	21	dep	activity	853:860	arg1	IC50					863:866	IC50	863:866	IC50 = 64.84 and 126.30 μM	863:888	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	1	22	theme	anti-A549	164:172	arg1	cells					186:190	anti-A549 lung cancer cells	164:190	anti-A549 lung cancer cells activity	164:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	4	23	theme	part	454:457	arg1	structure					459:467	the part structure	450:467	the part structure of PCp-I	450:476	Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified.					
31623207	1	24	dep	heteroglycan	127:138	arg1	PCp-II					151:156	PCp-II	151:156	PCp-II	151:156	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	24	dep	heteroglycan	127:138	arg1	PCp-I					141:145	PCp-I	141:145	PCp-I	141:145	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	25	theme	lung	174:177	arg1	cells					186:190	anti-A549 lung cancer cells	164:190	anti-A549 lung cancer cells activity	164:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	3	26	theme	same	335:338	arg1	composition					355:365	the same monosaccharide composition	331:365	the same monosaccharide composition	331:365	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	5	27	theme	scanning	509:516	arg1	SEM					539:541	SEM	539:541	SEM	539:541	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	5	27	theme	scanning	509:516	arg1	microscope					527:536	scanning electron microscope	509:536	scanning electron microscope (SEM)	509:542	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	1	28	theme	cancer	179:184	arg1	cells					186:190	anti-A549 lung cancer cells	164:190	anti-A549 lung cancer cells activity	164:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	0	29	theme	corylifolia	38:48	arg1	L					50:50	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	L	50:50	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	1	30	dep	Psoralea	220:227	arg1	L					241:241	L	241:241	Psoralea corylifolia L	220:241	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	30	dep	Psoralea	220:227	arg1	corylifolia					229:239	Psoralea corylifolia L	220:241	Psoralea corylifolia L	220:241	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	31	attach	isolated	206:213	arg1	Psoralea					220:227	Psoralea	220:227	Psoralea	220:227	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	31	attach	isolated	206:213	arg2	heteroglycan					127:138	Two novel water soluble heteroglycan	103:138	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity	103:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	1	32	theme	cells	186:190	arg1	activity					192:199	anti-A549 lung cancer cells activity	164:199	anti-A549 lung cancer cells activity	164:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	0	33	theme	Psoralea	29:36	arg1	L					50:50	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	L	50:50	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	3	34	theme	molar	378:382	arg1	ratios					384:389	their molar ratios	372:389	their molar ratios	372:389	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	7	35	theme	anti-A549	825:833	arg1	cells					847:851	anti-A549 lung cancer cells	825:851	significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM)	813:889	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	3	36	contain	had	327:329	arg1	PCp-II					320:325	PCp-II	320:325	PCp-II	320:325	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	3	36	contain	had	327:329	arg2	composition					355:365	the same monosaccharide composition	331:365	the same monosaccharide composition	331:365	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	3	36	contain	had	327:329	arg1	PCp-I					310:314	PCp-I	310:314	PCp-I	310:314	PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different.					
31623207	1	37	theme	novel	107:111	arg1	heteroglycan					127:138	Two novel water soluble heteroglycan	103:138	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity	103:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	6	38	theme	good	749:752	arg1	stability					762:770	good thermal stability	749:770	good thermal stability	749:770	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	5	39	theme	electron	518:525	arg1	SEM					539:541	SEM	539:541	SEM	539:541	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	5	39	theme	electron	518:525	arg1	microscope					527:536	scanning electron microscope	509:536	scanning electron microscope (SEM)	509:542	The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved.					
31623207	0	40	dep	Activity	84:91	arg1	Polysaccharides					10:24	Two Novel Polysaccharides	0:24	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells	0:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	1	41	theme	soluble	119:125	arg1	heteroglycan					127:138	Two novel water soluble heteroglycan	103:138	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity	103:199	Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L.					
31623207	0	42	theme	Lung	66:69	arg1	Cells					78:82	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	Cells	78:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	7	43	theme	=	868:868	arg1	IC50					863:866	IC50	863:866	IC50 = 64.84 and 126.30 μM	863:888	Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.					
31623207	0	44	theme	anti-A549	56:64	arg1	Cells					78:82	Psoralea corylifolia L and anti-A549 Lung Cancer Cells	29:82	Cells	78:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31623207	6	45	theme	calorimetry	695:705	arg1	results					645:651	The results	641:651	The results of thermogravimetry-differential scanning calorimetry (TG-DSC)	641:714	The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability.					
31623207	0	46	theme	Novel	4:8	arg1	Polysaccharides					10:24	Two Novel Polysaccharides	0:24	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells	0:82	Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro.					
31862050	6	0	theme	bridging	1316:1323	arg1	effects					1340:1346	bridging and enmeshment effects	1316:1346	bridging and enmeshment effects	1316:1346	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	6	1	theme	agminated	1226:1234	arg1	flocs					1236:1240	smaller and more agminated flocs	1209:1240	smaller and more agminated flocs with great recovery ability	1209:1268	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	1	2	theme	CuO	516:518	arg1	nanoparticles					520:532	CuO nanoparticles	516:532	CuO nanoparticles	516:532	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	0	3	contain	have	199:202	arg2	impacts					212:218	serious impacts	204:218	serious impacts	204:218	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	3	contain	have	199:202	arg1	removal					12:18	Synchronous removal	0:18	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides	0:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	3	4	theme	PACl-Ep	778:784	arg1	systems					786:792	the three PACl-Ep systems	768:792	the three PACl-Ep systems	768:792	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	3	5	theme	highest	813:819	arg1	efficiencies					829:840	the highest removal efficiencies	809:840	the highest removal efficiencies for turbidity and Cu2+	809:863	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	5	6	theme	Al	1076:1077	arg1	species					1079:1085	the Al species	1072:1085	all the Al species coagulants	1068:1096	For all the Al species coagulants, alkalescent conditions were more conducive to removing Cu and HA compared to acidic conditions.					
31862050	6	7	dep	systems	1307:1313	arg1	cooperated					1348:1357	cooperated	1348:1357	cooperated with the chelated reticular structure formed by the Ep and Al species	1348:1427	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	3	8	theme	removal	821:827	arg1	efficiencies					829:840	the highest removal efficiencies	809:840	the highest removal efficiencies for turbidity and Cu2+	809:863	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	7	9	theme	hydroxide	1478:1486	arg1	precipitates					1488:1499	hydroxide precipitates	1478:1499	hydroxide precipitates	1478:1499	Similarly, due to the increased hydrolysis and hydroxide precipitates, flocs formed under the condition of alkalescence were smaller, denser and stronger compared with weakly acidic conditions.					
31862050	6	10	theme	PAClc-Ep	1298:1305	arg1	systems					1307:1313	PAClb-Ep and PAClc-Ep systems	1285:1313	PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species)	1285:1428	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	6	11	theme	smaller	1209:1215	arg1	flocs					1236:1240	smaller and more agminated flocs	1209:1240	smaller and more agminated flocs with great recovery ability	1209:1268	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	1	12	theme	polyaluminum	441:452	arg1	PACl					464:467	PACl	464:467	PACl	464:467	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	1	12	theme	polyaluminum	441:452	arg1	chloride					454:461	polyaluminum chloride	441:461	polyaluminum chloride (PACl) (denoted as PACl-Ep)	441:489	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	4	13	theme	Higher	931:936	arg1	contents					938:945	Higher contents	931:945	Higher contents of Alb and Alc	931:960	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	0	14	theme	species	118:124	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	14	theme	species	118:124	arg1	removal					12:18	Synchronous removal	0:18	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides	0:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	14	theme	species	118:124	arg1	Effect					105:110	Effect	105:110	Effect of Al species	105:124	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	1	15	theme	innovative	351:360	arg1	polysaccharides					399:413	Enteromorpha polysaccharides	386:413	Enteromorpha polysaccharides (Ep)	386:418	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	1	15	theme	innovative	351:360	arg1	coagulant					371:379	an innovative recycled coagulant aid	348:383	an innovative recycled coagulant aid	348:383	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	2	16	theme	coagulants	600:609	arg1	influence					566:574	The influence	562:574	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms	562:715	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	0	17	theme	aquatic	245:251	arg1	environment					253:263	the aquatic environment	241:263	the aquatic environment	241:263	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	18	theme	composite	321:329	arg1	contaminants					331:342	composite contaminants	321:342	composite contaminants	321:342	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	19	theme	Al	115:116	arg1	species					118:124	Al species	115:124	Al species	115:124	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	1	20	theme	recycled	362:369	arg1	polysaccharides					399:413	Enteromorpha polysaccharides	386:413	Enteromorpha polysaccharides (Ep)	386:418	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	1	20	theme	recycled	362:369	arg1	coagulant					371:379	an innovative recycled coagulant aid	348:383	an innovative recycled coagulant aid	348:383	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	0	21	theme	serious	204:210	arg1	impacts					212:218	serious impacts	204:218	serious impacts	204:218	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	2	22	theme	reaction	697:704	arg1	mechanisms					706:715	reaction mechanisms	697:715	reaction mechanisms	697:715	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	4	23	theme	Alc	958:960	arg1	contents					938:945	Higher contents	931:945	Higher contents of Alb and Alc	931:960	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	2	24	theme	Al	589:590	arg1	species					592:598	different Al species	579:598	different Al species coagulants (AlCl3, PAClb and PAClc)	579:634	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	6	25	theme	enmeshment	1329:1338	arg1	effects					1340:1346	bridging and enmeshment effects	1316:1346	bridging and enmeshment effects	1316:1346	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	0	26	theme	Synchronous	0:10	arg1	removal					12:18	Synchronous removal	0:18	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides	0:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	2	27	theme	different	579:587	arg1	species					592:598	different Al species	579:598	different Al species coagulants (AlCl3, PAClb and PAClc)	579:634	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	7	28	theme	alkalescence	1538:1549	arg1	condition					1525:1533	the condition	1521:1533	the condition of alkalescence	1521:1549	Similarly, due to the increased hydrolysis and hydroxide precipitates, flocs formed under the condition of alkalescence were smaller, denser and stronger compared with weakly acidic conditions.					
31862050	3	29	theme	best	874:877	arg1	effect					893:898	the best UV254 removal effect	870:898	the best UV254 removal effect	870:898	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	0	30	theme	CuO	23:25	arg1	nanoparticles					27:39	CuO nanoparticles	23:39	CuO nanoparticles	23:39	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	7	31	theme	acidic	1606:1611	arg1	conditions					1613:1622	acidic conditions	1606:1622	acidic conditions	1606:1622	Similarly, due to the increased hydrolysis and hydroxide precipitates, flocs formed under the condition of alkalescence were smaller, denser and stronger compared with weakly acidic conditions.					
31862050	0	32	theme	pH.	130:132	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	3	33	theme	UV254	879:883	arg1	effect					893:898	the best UV254 removal effect	870:898	the best UV254 removal effect	870:898	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	6	34	theme	chelated	1368:1375	arg1	structure					1387:1395	the chelated reticular structure	1364:1395	the chelated reticular structure formed by the Ep and Al species	1364:1427	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	3	35	theme	removal	885:891	arg1	effect					893:898	the best UV254 removal effect	870:898	the best UV254 removal effect	870:898	Results showed that in the three PACl-Ep systems, PAClb-Ep gave the highest removal efficiencies for turbidity and Cu2+, and the best UV254 removal effect was reached by using PAClc-Ep.					
31862050	4	36	theme	sweeping	1046:1053	arg1	effects					1055:1061	sweeping effects	1046:1061	sweeping effects	1046:1061	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	2	37	from	influence	566:574	arg1	mechanisms					706:715	reaction mechanisms	697:715	reaction mechanisms	697:715	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	37	from	influence	566:574	arg1	performance					664:674	coagulation performance	652:674	coagulation performance	652:674	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	37	from	influence	566:574	arg1	properties					682:691	floc properties	677:691	floc properties	677:691	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	0	38	theme	oxide	141:145	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	39	theme	nanoparticles	27:39	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	39	theme	nanoparticles	27:39	arg1	removal					12:18	Synchronous removal	0:18	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides	0:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	39	theme	nanoparticles	27:39	arg1	Effect					105:110	Effect	105:110	Effect of Al species	105:124	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	5	40	theme	species	1079:1085	arg1	coagulants					1087:1096	all the Al species coagulants	1068:1096	all the Al species coagulants	1068:1096	For all the Al species coagulants, alkalescent conditions were more conducive to removing Cu and HA compared to acidic conditions.					
31862050	0	41	theme	Copper	134:139	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	1	42	theme	Enteromorpha	386:397	arg1	Ep					416:417	Ep	416:417	Ep	416:417	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	1	42	theme	Enteromorpha	386:397	arg1	polysaccharides					399:413	Enteromorpha polysaccharides	386:413	Enteromorpha polysaccharides (Ep)	386:418	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	1	42	theme	Enteromorpha	386:397	arg1	coagulant					371:379	an innovative recycled coagulant aid	348:383	an innovative recycled coagulant aid	348:383	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	2	43	dep	coagulants	600:609	arg1	PAClc					629:633	PAClc	629:633	PAClc	629:633	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	43	dep	coagulants	600:609	arg1	PAClb					619:623	PAClb	619:623	PAClb	619:623	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	43	dep	coagulants	600:609	arg1	AlCl3					612:616	AlCl3	612:616	AlCl3	612:616	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	43	dep	coagulants	600:609	arg1	coagulants					600:609	different Al species coagulants	579:609	different Al species coagulants (AlCl3, PAClb and PAClc)	579:634	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	0	44	theme	humic	297:301	arg1	acid					303:306	humic acid	297:306	humic acid (HA)	297:311	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	44	theme	humic	297:301	arg1	HA					309:310	HA	309:310	HA	309:310	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	6	45	theme	PAClb-Ep	1285:1292	arg1	systems					1307:1313	PAClb-Ep and PAClc-Ep systems	1285:1313	PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species)	1285:1428	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	4	46	theme	Alb	950:952	arg1	contents					938:945	Higher contents	931:945	Higher contents of Alb and Alc	931:960	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	1	47	dep	coagulant	371:379	arg1	aid					381:383	aid	381:383	aid	381:383	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	6	48	theme	recovery	1253:1260	arg1	ability					1262:1268	great recovery ability	1247:1268	great recovery ability	1247:1268	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	7	49	theme	increased	1453:1461	arg1	hydrolysis					1463:1472	the increased hydrolysis	1449:1472	the increased hydrolysis	1449:1472	Similarly, due to the increased hydrolysis and hydroxide precipitates, flocs formed under the condition of alkalescence were smaller, denser and stronger compared with weakly acidic conditions.					
31862050	0	50	theme	Cu2+	45:48	arg1	nanomaterials					147:159	pH. Copper oxide nanomaterials	130:159	pH. Copper oxide nanomaterials	130:159	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	50	theme	Cu2+	45:48	arg1	removal					12:18	Synchronous removal	0:18	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides	0:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	0	50	theme	Cu2+	45:48	arg1	Effect					105:110	Effect	105:110	Effect of Al species	105:124	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	6	51	theme	great	1247:1251	arg1	ability					1262:1268	great recovery ability	1247:1268	great recovery ability	1247:1268	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	2	52	theme	species	592:598	arg1	PAClc					629:633	PAClc	629:633	PAClc	629:633	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	52	theme	species	592:598	arg1	PAClb					619:623	PAClb	619:623	PAClb	619:623	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	52	theme	species	592:598	arg1	AlCl3					612:616	AlCl3	612:616	AlCl3	612:616	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	52	theme	species	592:598	arg1	coagulants					600:609	different Al species coagulants	579:609	different Al species coagulants (AlCl3, PAClb and PAClc)	579:634	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	2	53	theme	pH	646:647	arg1	influence					566:574	The influence	562:574	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms	562:715	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	5	54	theme	alkalescent	1099:1109	arg1	conditions					1111:1120	alkalescent conditions	1099:1120	alkalescent conditions	1099:1120	For all the Al species coagulants, alkalescent conditions were more conducive to removing Cu and HA compared to acidic conditions.					
31862050	2	55	theme	water	640:644	arg1	pH					646:647	water pH	640:647	water pH	640:647	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	1	56	dep	chloride	454:461	arg1	denoted					471:477	denoted	471:477	denoted as PACl-Ep	471:488	As an innovative recycled coagulant aid, Enteromorpha polysaccharides (Ep) were associated with polyaluminum chloride (PACl) (denoted as PACl-Ep) to simultaneously remove CuO nanoparticles, Cu2+ and HA in this study.					
31862050	6	57	dep	Ep	1411:1412	arg1	species					1421:1427	species	1421:1427	species	1421:1427	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	4	58	theme	great	977:981	arg1	performance					995:1005	great coagulation performance	977:1005	great coagulation performance	977:1005	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	2	59	theme	coagulation	652:662	arg1	performance					664:674	coagulation performance	652:674	coagulation performance	652:674	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	6	60	with	flocs	1236:1240	arg1	ability					1262:1268	great recovery ability	1247:1268	great recovery ability	1247:1268	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	0	61	theme	chloride-Enteromorpha	66:86	arg1	polysaccharides					88:102	polyaluminum chloride-Enteromorpha polysaccharides	53:102	polyaluminum chloride-Enteromorpha polysaccharides	53:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	6	62	theme	reticular	1377:1385	arg1	structure					1387:1395	the chelated reticular structure	1364:1395	the chelated reticular structure formed by the Ep and Al species	1364:1427	Additionally, smaller and more agminated flocs with great recovery ability were formed by PAClb-Ep and PAClc-Ep systems (bridging and enmeshment effects cooperated with the chelated reticular structure formed by the Ep and Al species).					
31862050	2	63	theme	floc	677:680	arg1	properties					682:691	floc properties	677:691	floc properties	677:691	The influence of different Al species coagulants (AlCl3, PAClb and PAClc) and water pH on coagulation performance, floc properties and reaction mechanisms was investigated in detail.					
31862050	0	64	theme	polyaluminum	53:64	arg1	polysaccharides					88:102	polyaluminum chloride-Enteromorpha polysaccharides	53:102	polyaluminum chloride-Enteromorpha polysaccharides	53:102	Synchronous removal of CuO nanoparticles and Cu2+ by polyaluminum chloride-Enteromorpha polysaccharides: Effect of Al species and pH. Copper oxide nanomaterials have been extensively applied and can have serious impacts when discharged into the aquatic environment, especially when complexed with humic acid (HA) to form composite contaminants.					
31862050	4	65	theme	coagulation	983:993	arg1	performance					995:1005	great coagulation performance	977:1005	great coagulation performance	977:1005	Higher contents of Alb and Alc contributed to great coagulation performance because of their stronger bridging and sweeping effects.					
31862050	5	66	theme	acidic	1176:1181	arg1	conditions					1183:1192	acidic conditions	1176:1192	acidic conditions	1176:1192	For all the Al species coagulants, alkalescent conditions were more conducive to removing Cu and HA compared to acidic conditions.					
31593720	7	0	theme	potential	1301:1309	arg1	bacteriostat					1323:1334	bacteriostat	1323:1334	bacteriostat	1323:1334	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	0	theme	potential	1301:1309	arg1	NPs					1259:1261	NPs	1259:1261	NPs from bamboo leaves	1259:1280	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	1	1	with	polysaccharides	179:193	arg1	weight					228:233	an average molecular weight	207:233	an average molecular weight of 5.77 × 103 Da	207:250	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	3	2	theme	Structural	476:485	arg1	analysis					487:494	Structural analysis	476:494	Structural analysis	476:494	Structural analysis indicated that NPs was formed by 1,4-β-linked xylp backbone.					
31593720	0	3	from	characterization	11:26	arg1	bamboo					106:111	bamboo	106:111	bamboo (Phyllostachys pubescens Mazel)	106:143	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	3	from	characterization	11:26	arg1	Mazel					138:142	Mazel	138:142	Mazel	138:142	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	1	4	theme	material	364:371	arg1	weight					350:355	weight	350:355	weight of raw material	350:371	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	4	5	theme	methylation	588:598	arg1	analysis					600:607	methylation analysis	588:607	methylation analysis	588:607	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	6	theme	Antioxidant	841:851	arg1	assays					853:858	Antioxidant assays	841:858	Antioxidant assays	841:858	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	5	7	theme	radical	993:999	arg1	scavenging					1001:1010	99.78% ABTS free radical scavenging	976:1010	99.78% ABTS free radical scavenging	976:1010	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	7	8	theme	natural	1315:1321	arg1	bacteriostat					1323:1334	bacteriostat	1323:1334	bacteriostat	1323:1334	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	8	theme	natural	1315:1321	arg1	NPs					1259:1261	NPs	1259:1261	NPs from bamboo leaves	1259:1280	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	4	9	theme	molar	700:704	arg1	ratio					706:710	the molar ratio	696:710	the molar ratio of 1.22:1.79:1.89:4.46	696:733	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	0	10	from	activity	59:66	arg1	bamboo					106:111	bamboo	106:111	bamboo (Phyllostachys pubescens Mazel)	106:143	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	10	from	activity	59:66	arg1	Mazel					138:142	Mazel	138:142	Mazel	138:142	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	7	11	dep	bamboo	1268:1273	arg1	leaves					1275:1280	leaves	1275:1280	leaves	1275:1280	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	6	12	theme	NPs	1179:1181	arg1	concentration					1183:1195	the NPs concentration	1175:1195	the NPs concentration	1175:1195	Moreover, NPs exhibited obvious growth inhibitory against E. coli, S. aureus and B. subtilis when the NPs concentration was in range of 0.50-50.0 mg/mL.					
31593720	4	13	theme	-Glc-	770:774	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	14	from	activity	908:915	arg1	manner					937:942	a dose-dependent manner	920:942	a dose-dependent manner	920:942	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	1	15	theme	raw	360:362	arg1	material					364:371	raw material	360:371	raw material	360:371	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	0	16	theme	polysaccharides	85:99	arg1	antioxidant					29:39	antioxidant	29:39	antioxidant	29:39	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	16	theme	polysaccharides	85:99	arg1	activity					59:66	antimicrobial activity	45:66	antimicrobial activity	45:66	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	16	theme	polysaccharides	85:99	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	4	17	theme	glycosidic	744:753	arg1	linkages					755:762	the glycosidic linkages	740:762	the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	740:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	18	theme	scavenging	1001:1010	arg1	rate					1012:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate	949:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0	949:1046	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	4	19	theme	side	679:682	arg1	chains					684:689	side chains	679:689	side chains	679:689	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	0	20	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	4	21	theme	-Gla-	798:802	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	22	theme	85.9	961:964	arg1	%					965:965	%	965:965	%	965:965	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	5	23	theme	high	903:906	arg1	activity					908:915	relatively high activity	892:915	relatively high activity in a dose-dependent manner	892:942	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	3	24	theme	1,4-β-linked	529:540	arg1	backbone					547:554	1,4-β-linked xylp backbone	529:554	1,4-β-linked xylp backbone	529:554	Structural analysis indicated that NPs was formed by 1,4-β-linked xylp backbone.					
31593720	4	25	theme	-Rha-	831:835	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	0	26	from	bamboo	106:111	arg1	antioxidant					29:39	antioxidant	29:39	antioxidant	29:39	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	26	from	bamboo	106:111	arg1	activity					59:66	antimicrobial activity	45:66	antimicrobial activity	45:66	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	26	from	bamboo	106:111	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	26	from	bamboo	106:111	arg1	polysaccharides					85:99	water-soluble polysaccharides	71:99	water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel)	71:143	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	3	27	theme	xylp	542:545	arg1	backbone					547:554	1,4-β-linked xylp backbone	529:554	1,4-β-linked xylp backbone	529:554	Structural analysis indicated that NPs was formed by 1,4-β-linked xylp backbone.					
31593720	7	28	from	bamboo	1268:1273	arg1	antioxidant					1340:1350	antioxidant	1340:1350	antioxidant	1340:1350	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	28	from	bamboo	1268:1273	arg1	bacteriostat					1323:1334	bacteriostat	1323:1334	bacteriostat	1323:1334	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	28	from	bamboo	1268:1273	arg1	NPs					1259:1261	NPs	1259:1261	NPs from bamboo leaves	1259:1280	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	1	29	theme	average	210:216	arg1	weight					228:233	an average molecular weight	207:233	an average molecular weight of 5.77 × 103 Da	207:250	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	0	30	theme	antimicrobial	45:57	arg1	activity					59:66	antimicrobial activity	45:66	antimicrobial activity	45:66	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	6	31	theme	0.50-50.0 mg/mL	1213:1227	arg1	range					1204:1208	range	1204:1208	range of 0.50-50.0 mg/mL	1204:1227	Moreover, NPs exhibited obvious growth inhibitory against E. coli, S. aureus and B. subtilis when the NPs concentration was in range of 0.50-50.0 mg/mL.					
31593720	5	32	theme	%	981:981	arg1	scavenging					1001:1010	99.78% ABTS free radical scavenging	976:1010	99.78% ABTS free radical scavenging	976:1010	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	2	33	dep	fractions	392:400	arg1	fractions					392:400	two fractions	388:400	two fractions (NPs-A and NPs-B)	388:418	Successively, two fractions (NPs-A and NPs-B) could be obtained after being eluted by DEAE-Sepharose.					
31593720	2	33	dep	fractions	392:400	arg1	NPs-B					413:417	NPs-B	413:417	NPs-B	413:417	Successively, two fractions (NPs-A and NPs-B) could be obtained after being eluted by DEAE-Sepharose.					
31593720	2	33	dep	fractions	392:400	arg1	NPs-A					403:407	NPs-A	403:407	NPs-A	403:407	Successively, two fractions (NPs-A and NPs-B) could be obtained after being eluted by DEAE-Sepharose.					
31593720	1	34	theme	molecular	218:226	arg1	weight					228:233	an average molecular weight	207:233	an average molecular weight of 5.77 × 103 Da	207:250	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	7	35	dep	bacteriostat	1323:1334	arg1	a					1299:1299	a	1299:1299	a	1299:1299	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	4	36	theme	Monosaccharide	557:570	arg1	composition					572:582	Monosaccharide composition	557:582	Monosaccharide composition	557:582	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	37	theme	ABTS	983:986	arg1	scavenging					1001:1010	99.78% ABTS free radical scavenging	976:1010	99.78% ABTS free radical scavenging	976:1010	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	4	38	theme	-Ara-	785:789	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	7	39	used	used	1291:1294	arg2	bacteriostat					1323:1334	bacteriostat	1323:1334	bacteriostat	1323:1334	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	39	used	used	1291:1294	arg2	NPs					1259:1261	NPs	1259:1261	NPs from bamboo leaves	1259:1280	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	7	39	used	used	1291:1294	arg2	antioxidant					1340:1350	antioxidant	1340:1350	antioxidant	1340:1350	Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.					
31593720	5	40	theme	99.78	976:980	arg1	%					981:981	%	981:981	%	981:981	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	5	41	theme	free	988:991	arg1	scavenging					1001:1010	99.78% ABTS free radical scavenging	976:1010	99.78% ABTS free radical scavenging	976:1010	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	4	42	theme	1→	837:838	arg1	linkages					755:762	the glycosidic linkages	740:762	the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	740:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	4	43	theme	-Rha-	811:815	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	1	44	dep	bamboo	271:276	arg1	leaves					310:315	leaves	310:315	leaves	310:315	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	5	45	theme	highest	953:959	arg1	rate					1012:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate	949:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0	949:1046	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	5	46	theme	%	965:965	arg1	DPPH					967:970	85.9% DPPH	961:970	85.9% DPPH	961:970	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	4	47	theme	1 → and → 2,4	817:829	arg1	1→					837:838	→4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→	767:838	1→	837:838	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	5	48	theme	DPPH	967:970	arg1	rate					1012:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate	949:1015	the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0	949:1046	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	1	49	attach	isolated	257:264	arg1	Mazel					303:307	Mazel	303:307	Mazel	303:307	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	1	49	attach	isolated	257:264	arg2	NPs					196:198	NPs	196:198	NPs	196:198	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	1	49	attach	isolated	257:264	arg2	polysaccharides					179:193	One water-soluble neutral polysaccharides	153:193	One water-soluble neutral polysaccharides (NPs)	153:199	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	1	49	attach	isolated	257:264	arg1	bamboo					271:276	bamboo	271:276	bamboo	271:276	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	4	50	theme	1.22:1.79:1.89:4.46	715:733	arg1	ratio					706:710	the molar ratio	696:710	the molar ratio of 1.22:1.79:1.89:4.46	696:733	Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→.					
31593720	1	51	theme	5.77 × 103 Da	238:250	arg1	weight					228:233	an average molecular weight	207:233	an average molecular weight of 5.77 × 103 Da	207:250	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	3	52	link	1,4-β-linked	529:540	arg1	backbone					547:554	1,4-β-linked xylp backbone	529:554	1,4-β-linked xylp backbone	529:554	Structural analysis indicated that NPs was formed by 1,4-β-linked xylp backbone.					
31593720	1	53	theme	water-soluble	157:169	arg1	NPs					196:198	NPs	196:198	NPs	196:198	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	1	53	theme	water-soluble	157:169	arg1	polysaccharides					179:193	One water-soluble neutral polysaccharides	153:193	One water-soluble neutral polysaccharides (NPs)	153:199	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	0	54	theme	water-soluble	71:83	arg1	polysaccharides					85:99	water-soluble polysaccharides	71:99	water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel)	71:143	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	55	from	antioxidant	29:39	arg1	bamboo					106:111	bamboo	106:111	bamboo (Phyllostachys pubescens Mazel)	106:143	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	0	55	from	antioxidant	29:39	arg1	Mazel					138:142	Mazel	138:142	Mazel	138:142	Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.					
31593720	5	56	theme	dose-dependent	922:935	arg1	manner					937:942	a dose-dependent manner	920:942	a dose-dependent manner	920:942	Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively.					
31593720	1	57	theme	neutral	171:177	arg1	NPs					196:198	NPs	196:198	NPs	196:198	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
31593720	1	57	theme	neutral	171:177	arg1	polysaccharides					179:193	One water-soluble neutral polysaccharides	153:193	One water-soluble neutral polysaccharides (NPs)	153:199	One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 103 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material.					
30035373	5	0	theme	soluble	758:764	arg1	arabinoxylan					766:777	soluble arabinoxylan	758:777	soluble arabinoxylan	758:777	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	4	1	theme	microbiota	685:694	arg1	diversity					702:710	microbiota alpha diversity	685:710	microbiota alpha diversity	685:710	Malts increased microbiota alpha diversity more than BSG and the extracts.					
30035373	5	2	theme	arabinoxylan	766:777	arg1	whereas					857:863	whereas	857:863	whereas	857:863	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	2	theme	arabinoxylan	766:777	arg1	related					783:789	related	783:789	related	783:789	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	2	theme	arabinoxylan	766:777	arg1	intake					748:753	The intake	744:753	The intake of soluble arabinoxylan	744:777	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	8	3	theme	fiber	1482:1486	arg1	extracts					1488:1495	purer fiber extracts	1476:1495	purer fiber extracts	1476:1495	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	8	4	theme	microbiota	1450:1459	arg1	diversity					1461:1469	microbiota diversity	1450:1469	microbiota diversity than purer fiber extracts	1450:1495	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	4	5	theme	alpha	696:700	arg1	diversity					702:710	microbiota alpha diversity	685:710	microbiota alpha diversity	685:710	Malts increased microbiota alpha diversity more than BSG and the extracts.					
30035373	5	6	from	related	783:789	arg1	cecum					842:846	the cecum	838:846	the cecum of rats	838:854	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	7	7	theme	higher	1347:1352	arg1	acid					1362:1365	a higher butyric acid	1345:1365	a higher butyric acid formation	1345:1375	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	2	8	theme	barley	311:316	arg1	fiber					326:330	barley dietary fiber	311:330	barley dietary fiber	311:330	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	2	9	theme	dietary	318:324	arg1	fiber					326:330	barley dietary fiber	311:330	barley dietary fiber	311:330	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	5	10	theme	butyrate-producing	940:957	arg1	bacteria					959:966	some potentially butyrate-producing bacteria	923:966	some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum)	923:1014	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	6	11	dep	CONCLUSION	1017:1026	arg1	demonstrates					1039:1050	demonstrates	1039:1050	demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties	1039:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	6	12	theme	fiber	1187:1191	arg1	properties					1193:1202	specific fiber properties	1178:1202	specific fiber properties	1178:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	7	13	theme	beer	1238:1241	arg1	production					1243:1252	beer production	1238:1252	beer production	1238:1252	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	3	14	theme	varying	545:551	arg1	arabinoxylan					610:621	insoluble arabinoxylan	600:621	insoluble arabinoxylan	600:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	14	theme	varying	545:551	arg1	β-glucan					564:571	β-glucan	564:571	β-glucan	564:571	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	14	theme	varying	545:551	arg1	arabinoxylan					582:593	soluble arabinoxylan	574:593	soluble arabinoxylan	574:593	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	14	theme	varying	545:551	arg1	amounts					553:559	varying amounts	545:559	varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan	545:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	8	15	from	mixture	1388:1394	arg1	malts					1415:1419	the malts	1411:1419	the malts	1411:1419	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	5	16	theme	rats	851:854	arg1	cecum					842:846	the cecum	838:846	the cecum of rats	838:854	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	8	17	theme	greater	1427:1433	arg1	importance					1435:1444	greater importance	1427:1444	greater importance for microbiota diversity than purer fiber extracts	1427:1495	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	3	18	theme	spent	492:496	arg1	BSG					505:507	BSG	505:507	BSG	505:507	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	18	theme	spent	492:496	arg1	grain					498:502	brewer's spent grain	483:502	brewer's spent grain (BSG)	483:508	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	6	19	theme	specific	1178:1185	arg1	properties					1193:1202	specific fiber properties	1178:1202	specific fiber properties	1178:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	3	20	theme	barley	469:474	arg1	malts					476:480	barley malts	469:480	barley malts	469:480	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	2	21	theme	physico-chemical	280:295	arg1	properties					297:306	different physico-chemical properties	270:306	different physico-chemical properties of barley dietary fiber	270:330	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	6	22	theme	butyrate-	1091:1099	arg1	bacteria					1126:1133	butyrate- and propionate-producing bacteria	1091:1133	butyrate- and propionate-producing bacteria	1091:1133	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	7	23	theme	microbiota	1314:1323	arg1	composition					1325:1335	the microbiota composition	1310:1335	the microbiota composition	1310:1335	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	3	24	theme	β-glucan	564:571	arg1	arabinoxylan					610:621	insoluble arabinoxylan	600:621	insoluble arabinoxylan	600:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	24	theme	β-glucan	564:571	arg1	β-glucan					564:571	β-glucan	564:571	β-glucan	564:571	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	24	theme	β-glucan	564:571	arg1	arabinoxylan					582:593	soluble arabinoxylan	574:593	soluble arabinoxylan	574:593	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	24	theme	β-glucan	564:571	arg1	amounts					553:559	varying amounts	545:559	varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan	545:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	25	theme	fiber	458:462	arg1	amounts					439:445	equal amounts	433:445	equal amounts of dietary fiber	433:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	25	theme	fiber	458:462	arg1	fiber					458:462	dietary fiber	450:462	dietary fiber	450:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	0	26	theme	Barley	0:5	arg1	Products					7:14	Barley Products	0:14	Barley Products of Different Fiber Composition	0:45	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	5	27	dep	related	783:789	arg1	whereas					857:863	whereas	857:863	whereas	857:863	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	27	dep	related	783:789	arg1	related					783:789	related	783:789	related	783:789	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	27	dep	related	783:789	arg1	intake					748:753	The intake	744:753	The intake of soluble arabinoxylan	744:777	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	3	28	contain	containing	422:431	arg1	diets					416:420	Seven diets	410:420	Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts	410:529	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	28	contain	containing	422:431	arg2	fiber					458:462	dietary fiber	450:462	dietary fiber	450:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	28	contain	containing	422:431	arg2	amounts					439:445	equal amounts	433:445	equal amounts of dietary fiber	433:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	1	29	theme	SCOPE	98:102	arg1	properties					126:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	5	30	theme	β-glucan	865:872	arg1	arabinoxylan					891:902	β-glucan and/or insoluble arabinoxylan	865:902	β-glucan and/or insoluble arabinoxylan	865:902	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	0	31	theme	Different	19:27	arg1	Composition					35:45	Different Fiber Composition	19:45	Different Fiber Composition	19:45	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	5	32	theme	propionic	810:818	arg1	formation					825:833	propionic acid formation	810:833	propionic acid formation	810:833	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	7	33	theme	butyric	1354:1360	arg1	acid					1362:1365	a higher butyric acid	1345:1365	a higher butyric acid formation	1345:1375	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	2	34	theme	fiber	326:330	arg1	properties					297:306	different physico-chemical properties	270:306	different physico-chemical properties of barley dietary fiber	270:330	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	1	35	theme	microbiota	192:201	arg1	composition					203:213	the microbiota composition	188:213	the microbiota composition	188:213	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	6	36	theme	malt	1161:1164	arg1	products					1166:1173	malt products	1161:1173	malt products of specific fiber properties	1161:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	1	37	theme	dietary	112:118	arg1	properties					126:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	1	38	theme	composition	203:213	arg1	profiling					175:183	the profiling	171:183	the profiling of the microbiota composition	171:213	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	0	39	theme	Composition	35:45	arg1	Products					7:14	Barley Products	0:14	Barley Products of Different Fiber Composition	0:45	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	7	40	theme	acid	1362:1365	arg1	formation					1367:1375	a higher butyric acid formation	1345:1375	a higher butyric acid formation	1345:1375	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	0	41	theme	Fiber	29:33	arg1	Composition					35:45	Different Fiber Composition	19:45	Different Fiber Composition	19:45	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	7	42	used	used	1285:1288	arg2	a					1220:1220	a	1220:1220	a	1220:1220	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	7	42	used	used	1285:1288	arg2	BSG					1215:1217	BSG	1215:1217	BSG	1215:1217	Moreover, BSG, a by product from beer production, added to malt can possibly be used to further modulate the microbiota composition, toward a higher butyric acid formation.					
30035373	3	43	theme	dietary	450:456	arg1	fiber					458:462	dietary fiber	450:462	dietary fiber	450:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	0	44	from	Composition	77:87	arg1	Rats					92:95	Rats	92:95	Rats	92:95	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	5	45	theme	acid	820:823	arg1	formation					825:833	propionic acid formation	810:833	propionic acid formation	810:833	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	46	from	cecum	842:846	arg1	whereas					857:863	whereas	857:863	whereas	857:863	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	46	from	cecum	842:846	arg1	related					783:789	related	783:789	related	783:789	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	46	from	cecum	842:846	arg1	intake					748:753	The intake	744:753	The intake of soluble arabinoxylan	744:777	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	5	47	dep	Lactobacillus	975:987	arg1	e.g.					969:972	e.g.	969:972	e.g.	969:972	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	3	48	theme	equal	433:437	arg1	amounts					439:445	equal amounts	433:445	equal amounts of dietary fiber	433:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	48	theme	equal	433:437	arg1	fiber					458:462	dietary fiber	450:462	dietary fiber	450:462	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	6	49	with	rats	1151:1154	arg1	products					1166:1173	malt products	1161:1173	malt products of specific fiber properties	1161:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	3	50	theme	soluble	574:580	arg1	arabinoxylan					582:593	soluble arabinoxylan	574:593	soluble arabinoxylan	574:593	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	1	51	theme	fiber	120:124	arg1	properties					126:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	8	52	theme	purer	1476:1480	arg1	extracts					1488:1495	purer fiber extracts	1476:1495	purer fiber extracts	1476:1495	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	3	53	theme	arabinoxylan	582:593	arg1	arabinoxylan					610:621	insoluble arabinoxylan	600:621	insoluble arabinoxylan	600:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	53	theme	arabinoxylan	582:593	arg1	β-glucan					564:571	β-glucan	564:571	β-glucan	564:571	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	53	theme	arabinoxylan	582:593	arg1	arabinoxylan					582:593	soluble arabinoxylan	574:593	soluble arabinoxylan	574:593	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	53	theme	arabinoxylan	582:593	arg1	amounts					553:559	varying amounts	545:559	varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan	545:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	54	dep	METHODS	390:396	arg1	given					641:645	given	641:645	were given to conventional rats	636:666	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	8	55	theme	fiber	1399:1403	arg1	mixture					1388:1394	A complex mixture	1378:1394	A complex mixture of fiber as in the malts	1378:1419	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30035373	2	56	theme	different	270:278	arg1	properties					297:306	different physico-chemical properties	270:306	different physico-chemical properties of barley dietary fiber	270:330	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	2	57	theme	microbiota	350:359	arg1	composition					361:371	the gut microbiota composition	342:371	the gut microbiota composition	342:371	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	1	58	theme	Several	104:110	arg1	properties					126:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties	98:135	SCOPE Several dietary fiber properties are suggested to be important for the profiling of the microbiota composition, but those characteristics are rather unclear.					
30035373	3	59	theme	conventional	650:661	arg1	rats					663:666	conventional rats	650:666	conventional rats	650:666	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	6	60	theme	properties	1193:1202	arg1	products					1166:1173	malt products	1161:1173	malt products of specific fiber properties	1161:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	2	61	theme	gut	346:348	arg1	composition					361:371	the gut microbiota composition	342:371	the gut microbiota composition	342:371	Whether different physico-chemical properties of barley dietary fiber influence the gut microbiota composition is investigated.					
30035373	6	62	theme	propionate-producing	1105:1124	arg1	bacteria					1126:1133	butyrate- and propionate-producing bacteria	1091:1133	butyrate- and propionate-producing bacteria	1091:1133	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	3	63	theme	insoluble	600:608	arg1	arabinoxylan					610:621	insoluble arabinoxylan	600:621	insoluble arabinoxylan	600:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	64	theme	barley	515:520	arg1	extracts					522:529	barley extracts	515:529	barley extracts	515:529	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	0	65	theme	Microbiota	66:75	arg1	Composition					77:87	Microbiota Composition	66:87	Microbiota Composition in Rats	66:95	Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats.					
30035373	6	66	theme	rats	1151:1154	arg1	cecum					1142:1146	the cecum	1138:1146	the cecum of rats with malt products of specific fiber properties	1138:1202	CONCLUSION This study demonstrates that there is a potential to stimulate butyrate- and propionate-producing bacteria in the cecum of rats with malt products of specific fiber properties.					
30035373	3	67	theme	arabinoxylan	610:621	arg1	arabinoxylan					610:621	insoluble arabinoxylan	600:621	insoluble arabinoxylan	600:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	67	theme	arabinoxylan	610:621	arg1	β-glucan					564:571	β-glucan	564:571	β-glucan	564:571	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	67	theme	arabinoxylan	610:621	arg1	arabinoxylan					582:593	soluble arabinoxylan	574:593	soluble arabinoxylan	574:593	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	3	67	theme	arabinoxylan	610:621	arg1	amounts					553:559	varying amounts	545:559	varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan	545:621	METHODS AND RESULTS Seven diets containing equal amounts of dietary fiber from barley malts, brewer's spent grain (BSG), and barley extracts, resulting in varying amounts of β-glucan, soluble arabinoxylan, and insoluble arabinoxylan in the diets were given to conventional rats.					
30035373	5	68	theme	insoluble	881:889	arg1	arabinoxylan					891:902	β-glucan and/or insoluble arabinoxylan	865:902	β-glucan and/or insoluble arabinoxylan	865:902	The intake of soluble arabinoxylan was related to Akkermansia and propionic acid formation in the cecum of rats, whereas β-glucan and/or insoluble arabinoxylan were attributed to some potentially butyrate-producing bacteria (e.g., Lactobacillus, Blautia, and Allobaculum).					
30035373	8	69	theme	complex	1380:1386	arg1	mixture					1388:1394	A complex mixture	1378:1394	A complex mixture of fiber as in the malts	1378:1419	A complex mixture of fiber as in the malts is of greater importance for microbiota diversity than purer fiber extracts.					
30465847	8	0	theme	LMPs	1320:1323	arg1	properties					1306:1315	the biological properties	1291:1315	the biological properties of LMPs	1291:1323	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	3	1	from	water	495:499	arg1	polysaccharides					448:462	pectic polysaccharides	441:462	pectic polysaccharides from citrus canning processing water	441:499	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	1	2	theme	valuable	174:181	arg1	source					197:202	a valuable and renewable source	172:202	a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides	172:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	4	3	theme	canning	568:574	arg1	water					587:591	citrus canning processing water	561:591	citrus canning processing water	561:591	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	8	4	theme	monosaccharide	1402:1415	arg1	composition					1417:1427	monosaccharide composition	1402:1427	monosaccharide composition	1402:1427	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	4	5	theme	pectic	524:529	arg1	polysaccharides					531:545	pectic polysaccharides	524:545	pectic polysaccharides recycled from citrus canning processing water	524:591	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	8	6	contain	possessed	1167:1175	arg1	LMP2					1162:1165	LMP2	1162:1165	LMP2	1162:1165	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	6	contain	possessed	1167:1175	arg1	LMP1					1153:1156	LMP1	1153:1156	LMP1	1153:1156	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	6	contain	possessed	1167:1175	arg2	activity					1199:1206	significant antitumor activity	1177:1206	significant antitumor activity	1177:1206	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	0	7	theme	antiproliferation	101:117	arg1	potentials					119:128	their antiproliferation potentials	95:128	their antiproliferation potentials	95:128	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	4	8	theme	citrus	561:566	arg1	water					587:591	citrus canning processing water	561:591	citrus canning processing water	561:591	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	2	9	theme	environmental	334:346	arg1	pollution					348:356	environmental pollution	334:356	environmental pollution	334:356	Upgrading these processing wastes can not only alleviate environmental pollution but also add value to the commodity's production.					
30465847	8	10	theme	Further	1026:1032	arg1	assay					1044:1048	Further antitumor assay	1026:1048	Further antitumor assay	1026:1048	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	11	theme	inhibitory	1219:1228	arg1	effect					1230:1235	the inhibitory effect	1215:1235	the inhibitory effect of LMP1	1215:1243	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	11	theme	inhibitory	1219:1228	arg1	higher					1249:1254	higher	1249:1254	higher	1249:1254	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	12	theme	structural	1343:1352	arg1	characteristics					1354:1368	structural characteristics	1343:1368	structural characteristics	1343:1368	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	12	theme	structural	1343:1352	arg1	weight					1391:1396	molecular weight	1381:1396	molecular weight	1381:1396	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	12	theme	structural	1343:1352	arg1	composition					1417:1427	monosaccharide composition	1402:1427	monosaccharide composition	1402:1427	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	13	theme	LMP1	1240:1243	arg1	effect					1230:1235	the inhibitory effect	1215:1235	the inhibitory effect of LMP1	1215:1243	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	13	theme	LMP1	1240:1243	arg1	higher					1249:1254	higher	1249:1254	higher	1249:1254	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	5	14	theme	size-exclusion	679:692	arg1	chromatography					694:707	size-exclusion chromatography	679:707	size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa	679:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	1	15	theme	renewable	187:195	arg1	source					197:202	a valuable and renewable source	172:202	a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides	172:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	0	16	theme	processing	55:64	arg1	water					66:70	citrus canning processing water	40:70	citrus canning processing water by Fenton reaction and their antiproliferation potentials	40:128	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	8	17	theme	antitumor	1189:1197	arg1	activity					1199:1206	significant antitumor activity	1177:1206	significant antitumor activity	1177:1206	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	1	18	contain	contains	163:170	arg1	water					157:161	Citrus canning processing water	131:161	Citrus canning processing water	131:161	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	1	18	contain	contains	163:170	arg2	source					197:202	a valuable and renewable source	172:202	a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides	172:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	8	19	theme	significant	1177:1187	arg1	activity					1199:1206	significant antitumor activity	1177:1206	significant antitumor activity	1177:1206	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	3	20	theme	citrus	469:474	arg1	water					495:499	citrus canning processing water	469:499	citrus canning processing water	469:499	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	0	21	theme	Pectic	0:5	arg1	oligosaccharides					7:22	Pectic oligosaccharides	0:22	Pectic oligosaccharides	0:22	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	8	22	with	comparison	1065:1074	arg1	activity					1138:1145	moderate antitumor activity	1119:1145	moderate antitumor activity	1119:1145	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	22	with	comparison	1065:1074	arg1	polysaccharide					1099:1112	the native pectic polysaccharide	1081:1112	the native pectic polysaccharide	1081:1112	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	3	23	theme	canning	476:482	arg1	water					495:499	citrus canning processing water	469:499	citrus canning processing water	469:499	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	8	24	theme	molecular	1381:1389	arg1	weight					1391:1396	molecular weight	1381:1396	molecular weight	1381:1396	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	6	25	theme	non-esterified	932:945	arg1	polysaccharides					947:961	homogalacturonans-enriched non-esterified polysaccharides	905:961	homogalacturonans-enriched non-esterified polysaccharides	905:961	Structure analyses showed that LMP1 were homogalacturonans-enriched non-esterified polysaccharides.					
30465847	3	26	theme	previous	413:420	arg1	study					422:426	a previous study	411:426	a previous study	411:426	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	4	27	theme	Fenton	627:632	arg1	system					634:639	an optimized Fenton system	614:639	an optimized Fenton system	614:639	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	8	28	theme	native	1085:1090	arg1	polysaccharide					1099:1112	the native pectic polysaccharide	1081:1112	the native pectic polysaccharide	1081:1112	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	1	29	theme	bioactive	223:231	arg1	compounds					233:241	bioactive compounds	223:241	bioactive compounds	223:241	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	1	29	theme	bioactive	223:231	arg1	polysaccharides					260:274	pectic polysaccharides	253:274	pectic polysaccharides	253:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	0	30	theme	canning	47:53	arg1	water					66:70	citrus canning processing water	40:70	citrus canning processing water by Fenton reaction and their antiproliferation potentials	40:128	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	4	31	theme	optimized	617:625	arg1	system					634:639	an optimized Fenton system	614:639	an optimized Fenton system	614:639	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	8	32	theme	antitumor	1034:1042	arg1	assay					1044:1048	Further antitumor assay	1026:1048	Further antitumor assay	1026:1048	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	1	33	theme	biopolymers	207:217	arg1	source					197:202	a valuable and renewable source	172:202	a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides	172:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	1	34	theme	compounds	233:241	arg1	source					197:202	a valuable and renewable source	172:202	a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides	172:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	3	35	theme	processing	484:493	arg1	water					495:499	citrus canning processing water	469:499	citrus canning processing water	469:499	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	0	36	theme	citrus	40:45	arg1	water					66:70	citrus canning processing water	40:70	citrus canning processing water by Fenton reaction and their antiproliferation potentials	40:128	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	4	37	theme	present	509:515	arg1	study					517:521	the present study	505:521	the present study	505:521	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	6	38	theme	homogalacturonans-enriched	905:930	arg1	polysaccharides					947:961	homogalacturonans-enriched non-esterified polysaccharides	905:961	homogalacturonans-enriched non-esterified polysaccharides	905:961	Structure analyses showed that LMP1 were homogalacturonans-enriched non-esterified polysaccharides.					
30465847	5	39	dep	chromatography	694:707	arg1	25 kDa < LMP5 < 100 kDa					821:843	25 kDa < LMP5 < 100 kDa	821:843	25 kDa < LMP5 < 100 kDa	821:843	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	5	39	dep	chromatography	694:707	arg1	LMP6 > 10 wDa					849:861	LMP6 > 10 wDa	849:861	LMP6 > 10 wDa	849:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	5	39	dep	chromatography	694:707	arg1	5 kDa < LMP3 < 12 kDa					774:794	5 kDa < LMP3 < 12 kDa	774:794	size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa	679:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	5	39	dep	chromatography	694:707	arg1	12 kDa < LMP4 < 25 kDa					797:818	12 kDa < LMP4 < 25 kDa	797:818	size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa	679:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	5	39	dep	chromatography	694:707	arg1	500 Da < LMP1 < 3 kDa					729:749	500 Da < LMP1 < 3 kDa	729:749	size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa	679:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	5	39	dep	chromatography	694:707	arg1	3 kDa < LMP2 < 5 kDa					752:771	3 kDa < LMP2 < 5 kDa	752:771	size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa	679:861	The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa.					
30465847	4	40	theme	processing	576:585	arg1	water					587:591	citrus canning processing water	561:591	citrus canning processing water	561:591	In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system.					
30465847	8	41	theme	pectic	1092:1097	arg1	polysaccharide					1099:1112	the native pectic polysaccharide	1081:1112	the native pectic polysaccharide	1081:1112	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	1	42	theme	Citrus	131:136	arg1	water					157:161	Citrus canning processing water	131:161	Citrus canning processing water	131:161	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	3	43	theme	pectic	441:446	arg1	polysaccharides					448:462	pectic polysaccharides	441:462	pectic polysaccharides from citrus canning processing water	441:499	In a previous study we recovered pectic polysaccharides from citrus canning processing water.					
30465847	8	44	theme	moderate	1119:1126	arg1	activity					1138:1145	moderate antitumor activity	1119:1145	moderate antitumor activity	1119:1145	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	1	45	theme	canning	138:144	arg1	water					157:161	Citrus canning processing water	131:161	Citrus canning processing water	131:161	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	1	46	theme	processing	146:155	arg1	water					157:161	Citrus canning processing water	131:161	Citrus canning processing water	131:161	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	1	47	theme	pectic	253:258	arg1	polysaccharides					260:274	pectic polysaccharides	253:274	pectic polysaccharides	253:274	Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides.					
30465847	0	48	theme	Fenton	75:80	arg1	reaction					82:89	Fenton reaction	75:89	Fenton reaction	75:89	Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials.					
30465847	2	49	theme	processing	293:302	arg1	wastes					304:309	these processing wastes	287:309	these processing wastes	287:309	Upgrading these processing wastes can not only alleviate environmental pollution but also add value to the commodity's production.					
30465847	8	50	theme	antitumor	1128:1136	arg1	activity					1138:1145	moderate antitumor activity	1119:1145	moderate antitumor activity	1119:1145	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	8	51	theme	biological	1295:1304	arg1	properties					1306:1315	the biological properties	1291:1315	the biological properties of LMPs	1291:1323	Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.					
30465847	6	52	theme	Structure	864:872	arg1	analyses					874:881	Structure analyses	864:881	Structure analyses	864:881	Structure analyses showed that LMP1 were homogalacturonans-enriched non-esterified polysaccharides.					
31196546	1	0	theme	gellan	144:149	arg1	exopolysaccharide					155:171	the gellan gum exopolysaccharide	140:171	the gellan gum exopolysaccharide	140:171	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	0	1	theme	protein	82:88	arg1	capture					90:96	protein capture	82:96	protein capture	82:96	Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture.					
31196546	1	2	theme	gum	151:153	arg1	exopolysaccharide					155:171	the gellan gum exopolysaccharide	140:171	the gellan gum exopolysaccharide	140:171	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	1	3	theme	oxygen	259:264	arg1	1:5					275:277	1:5	275:277	1:5	275:277	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	1	3	theme	oxygen	259:264	arg1	content					266:272	the oxygen content	255:272	the oxygen content (1:5)	255:278	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	6	4	with	interactions	1090:1101	arg1	microspheres					1114:1125	these microspheres	1108:1125	these microspheres	1108:1125	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	2	5	with	dissolution	444:454	arg1	water					471:475	distilled water	461:475	distilled water	461:475	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	2	6	theme	FTIR	281:284	arg1	data					294:297	FTIR and NMR data	281:297	data	294:297	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	3	7	theme	microsphere	524:534	arg1	formulation					536:546	Gellan microsphere formulation	517:546	Gellan microsphere formulation by water-in-oil emulsion	517:571	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	6	8	theme	strength	962:969	arg1	manipulation					940:951	The manipulation	936:951	The manipulation of ionic strength or pH on the binding and elution steps	936:1008	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	4	9	theme	gellan	742:747	arg1	750 rpm					764:770	750 rpm	764:770	750 rpm	764:770	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	9	theme	gellan	742:747	arg1	concentration					749:761	1.27% gellan concentration	736:761	1.27% gellan concentration	736:761	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	9	theme	gellan	742:747	arg1	92.54 °C					776:783	92.54 °C	776:783	92.54 °C	776:783	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	1	10	theme	exopolysaccharide	155:171	arg1	biosynthesis					124:135	the biosynthesis	120:135	the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation	120:215	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	3	11	theme	different	677:685	arg1	counter-ions					687:698	different counter-ions	677:698	different counter-ions	677:698	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	2	12	with	precipitation	478:490	arg1	acetonitrile					497:508	acetonitrile (1:3)	497:514	acetonitrile (1:3)	497:514	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	4	13	theme	%	740:740	arg1	750 rpm					764:770	750 rpm	764:770	750 rpm	764:770	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	13	theme	%	740:740	arg1	concentration					749:761	1.27% gellan concentration	736:761	1.27% gellan concentration	736:761	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	13	theme	%	740:740	arg1	92.54 °C					776:783	92.54 °C	776:783	92.54 °C	776:783	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	6	14	theme	electrostatic	1064:1076	arg1	interactions					1090:1101	electrostatic or affinity interactions	1064:1101	interactions	1090:1101	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	2	15	theme	each	424:427	arg1	200 mL					417:422	200 mL each one	417:431	200 mL each one	417:431	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	2	15	theme	each	424:427	arg1	ether					410:414	ether	410:414	ether (200 mL each one)	410:432	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	5	16	from	SEM	914:916	arg1	results					876:882	the most satisfactory results	854:882	the most satisfactory results from semi-optical microscopy, SEM and EDX analysis	854:933	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	1	17	theme	Sphingomonas	178:189	arg1	paucimobilis					191:202	Sphingomonas paucimobilis	178:202	Sphingomonas paucimobilis fermentation	178:215	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	6	18	theme	ionic	956:960	arg1	strength					962:969	ionic strength	956:969	ionic strength	956:969	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	4	19	theme	optimal	705:711	arg1	point					713:717	The optimal point	701:717	The optimal point	701:717	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	20	theme	1.27	736:739	arg1	%					740:740	%	740:740	%	740:740	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	1	21	theme	paucimobilis	191:202	arg1	fermentation					204:215	Sphingomonas paucimobilis fermentation	178:215	Sphingomonas paucimobilis fermentation	178:215	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	6	22	from	manipulation	940:951	arg1	steps					1004:1008	the binding and elution steps	980:1008	steps	1004:1008	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	5	23	from	microscopy	902:911	arg1	results					876:882	the most satisfactory results	854:882	the most satisfactory results from semi-optical microscopy, SEM and EDX analysis	854:933	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	2	24	theme	NMR	290:292	arg1	data					294:297	FTIR and NMR data	281:297	data	294:297	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	6	25	theme	different	1028:1036	arg1	proteins					1044:1051	different model proteins	1028:1051	different model proteins	1028:1051	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	5	26	theme	EDX	922:924	arg1	analysis					926:933	EDX analysis	922:933	EDX analysis	922:933	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	1	27	theme	present	106:112	arg1	work					114:117	the present work	102:117	the present work	102:117	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	0	28	theme	polysaccharide	37:50	arg1	isolation					17:25	isolation	17:25	isolation	17:25	Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture.					
31196546	0	28	theme	polysaccharide	37:50	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis	0:11	Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture.					
31196546	6	29	theme	model	1038:1042	arg1	proteins					1044:1051	different model proteins	1028:1051	different model proteins	1028:1051	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	0	30	theme	gellan	30:35	arg1	polysaccharide					37:50	gellan polysaccharide	30:50	gellan polysaccharide	30:50	Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture.					
31196546	5	31	with	Microspheres	805:816	arg1	nickel					823:828	nickel	823:828	nickel	823:828	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	6	32	theme	pH	974:975	arg1	manipulation					940:951	The manipulation	936:951	The manipulation of ionic strength or pH on the binding and elution steps	936:1008	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	3	33	theme	water-in-oil	551:562	arg1	emulsion					564:571	water-in-oil emulsion	551:571	water-in-oil emulsion	551:571	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	2	34	from	medium	365:370	arg1	gellan					345:350	gellan	345:350	gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3)	345:514	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	5	35	theme	semi-optical	889:900	arg1	microscopy					902:911	semi-optical microscopy	889:911	semi-optical microscopy	889:911	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	2	36	dep	dissolution	444:454	arg1	permeate					435:442	permeate	435:442	permeate	435:442	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	4	37	theme	oil	788:790	arg1	temperature					792:802	oil temperature	788:802	oil temperature	788:802	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	1	38	from	fermentation	204:215	arg1	biosynthesis					124:135	the biosynthesis	120:135	the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation	120:215	In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5).					
31196546	5	39	from	analysis	926:933	arg1	results					876:882	the most satisfactory results	854:882	the most satisfactory results from semi-optical microscopy, SEM and EDX analysis	854:933	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
31196546	3	40	theme	composite	603:611	arg1	design					613:618	central composite design	595:618	central composite design	595:618	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	2	41	theme	following	313:321	arg1	procedures					323:332	the following procedures	309:332	the following procedures	309:332	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	3	42	theme	Gellan	517:522	arg1	formulation					536:546	Gellan microsphere formulation	517:546	Gellan microsphere formulation by water-in-oil emulsion	517:571	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	6	43	theme	elution	996:1002	arg1	steps					1004:1008	the binding and elution steps	980:1008	steps	1004:1008	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	2	44	dep	gellan	345:350	arg1	filtration					373:382	filtration	373:382	gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3)	345:514	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	2	44	dep	gellan	345:350	arg1	precipitation					478:490	precipitation	478:490	gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3)	345:514	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	2	44	dep	gellan	345:350	arg1	dissolution					444:454	permeate dissolution	435:454	gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3)	345:514	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	2	44	dep	gellan	345:350	arg1	washing					385:391	washing	385:391	washing	385:391	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	6	45	theme	affinity	1081:1088	arg1	interactions					1090:1101	electrostatic or affinity interactions	1064:1101	interactions	1090:1101	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	4	46	theme	temperature	792:802	arg1	750 rpm					764:770	750 rpm	764:770	750 rpm	764:770	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	46	theme	temperature	792:802	arg1	concentration					749:761	1.27% gellan concentration	736:761	1.27% gellan concentration	736:761	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	4	46	theme	temperature	792:802	arg1	92.54 °C					776:783	92.54 °C	776:783	92.54 °C	776:783	The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 °C of oil temperature.					
31196546	2	47	theme	culture	357:363	arg1	medium					365:370	culture medium	357:370	culture medium	357:370	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	6	48	theme	binding	984:990	arg1	steps					1004:1008	the binding and elution steps	980:1008	steps	1004:1008	The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.					
31196546	2	49	theme	distilled	461:469	arg1	water					471:475	distilled water	461:475	distilled water	461:475	FTIR and NMR data indicated the following procedures to recover gellan from culture medium: filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3).					
31196546	3	50	theme	central	595:601	arg1	design					613:618	central composite design	595:618	central composite design	595:618	Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (≈ 300 μm), and reinforced with different counter-ions.					
31196546	5	51	theme	satisfactory	863:874	arg1	results					876:882	the most satisfactory results	854:882	the most satisfactory results from semi-optical microscopy, SEM and EDX analysis	854:933	Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis.					
29727640	1	0	theme	antioxidant	176:186	arg1	activity					188:195	antioxidant activity	176:195	antioxidant activity	176:195	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	7	1	theme	notable	972:978	arg1	activity					1005:1012	notable α-glucosidase inhibition activity	972:1012	notable α-glucosidase inhibition activity	972:1012	In addition, UA-CPs showed notable α-glucosidase inhibition activity.					
29727640	3	2	theme	EUA	537:539	arg1	methods					541:547	EUA methods	537:547	EUA methods	537:547	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	2	3	theme	hot	319:321	arg1	HW					341:342	HW	341:342	HW	341:342	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	3	theme	hot	319:321	arg1	extraction					329:338	hot water extraction	319:338	hot water extraction (HW)	319:343	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	5	4	theme	antihyperglycemic	739:755	arg1	activities					757:766	antihyperglycemic activities	739:766	antihyperglycemic activities	739:766	CPs showed antioxidant activities and antihyperglycemic activities in a concentration-dependent manner.					
29727640	6	5	theme	acid	931:934	arg1	content					936:942	higher uronic acid content	917:942	higher uronic acid content	917:942	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	7	6	theme	α-glucosidase	980:992	arg1	activity					1005:1012	notable α-glucosidase inhibition activity	972:1012	notable α-glucosidase inhibition activity	972:1012	In addition, UA-CPs showed notable α-glucosidase inhibition activity.					
29727640	4	7	from	difference	663:672	arg1	content					692:698	monosaccharide content	677:698	monosaccharide content	677:698	The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content.					
29727640	8	8	theme	ultrasonic-assisted	1044:1062	arg1	technology					1075:1084	ultrasonic-assisted extraction technology	1044:1084	ultrasonic-assisted extraction technology	1044:1084	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	2	9	theme	ultrasonic-assisted	346:364	arg1	UA					378:379	UA	378:379	UA	378:379	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	9	theme	ultrasonic-assisted	346:364	arg1	extraction					366:375	ultrasonic-assisted extraction	346:375	ultrasonic-assisted extraction (UA)	346:380	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	8	10	theme	extraction	1121:1130	arg1	yields					1132:1137	the extraction yields	1117:1137	the extraction yields of the polysaccharides	1117:1160	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	7	11	theme	inhibition	994:1003	arg1	activity					1005:1012	notable α-glucosidase inhibition activity	972:1012	notable α-glucosidase inhibition activity	972:1012	In addition, UA-CPs showed notable α-glucosidase inhibition activity.					
29727640	2	12	theme	extract	306:312	arg1	CPs					314:316	extract CPs	306:316	extract CPs	306:316	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	6	13	theme	higher	917:922	arg1	content					936:942	higher uronic acid content	917:942	higher uronic acid content	917:942	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	6	14	theme	antioxidant	829:839	arg1	capacity					841:848	better antioxidant capacity	822:848	better antioxidant capacity	822:848	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	1	15	theme	antihyperglycemic	201:217	arg1	activity					219:226	antihyperglycemic activity	201:226	antihyperglycemic activity	201:226	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	6	16	dep	exhibited	812:820	arg1	related					873:879	related	873:879	related	873:879	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	6	17	theme	uronic	924:929	arg1	content					936:942	higher uronic acid content	917:942	higher uronic acid content	917:942	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	6	18	theme	better	822:827	arg1	capacity					841:848	better antioxidant capacity	822:848	better antioxidant capacity	822:848	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	2	19	used	used	298:301	arg2	techniques					282:291	Four techniques	277:291	Four techniques	277:291	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	20	theme	enzyme-assisted	383:397	arg1	extraction					399:408	enzyme-assisted extraction	383:408	enzyme-assisted extraction (EA)	383:413	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	20	theme	enzyme-assisted	383:397	arg1	EA					411:412	EA	411:412	EA	411:412	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	0	21	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques.					
29727640	4	22	theme	significant	651:661	arg1	difference					663:672	a significant difference	649:672	a significant difference in monosaccharide content	649:698	The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content.					
29727640	8	23	theme	polysaccharides	1146:1160	arg1	yields					1132:1137	the extraction yields	1117:1137	the extraction yields of the polysaccharides	1117:1160	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	2	24	theme	water	323:327	arg1	HW					341:342	HW	341:342	HW	341:342	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	24	theme	water	323:327	arg1	extraction					329:338	hot water extraction	319:338	hot water extraction (HW)	319:343	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	25	theme	enzyme-ultrasonic-assisted	419:444	arg1	EUA					458:460	EUA	458:460	EUA	458:460	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	2	25	theme	enzyme-ultrasonic-assisted	419:444	arg1	extraction					446:455	enzyme-ultrasonic-assisted extraction	419:455	enzyme-ultrasonic-assisted extraction (EUA)	419:461	Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA).					
29727640	3	26	contain	had	559:561	arg1	CPs					500:502	CPs	500:502	CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs)	500:557	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	3	26	contain	had	559:561	arg2	yields					581:586	higher extraction yields	563:586	higher extraction yields	563:586	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	1	27	theme	extraction	119:128	arg1	techniques					130:139	extraction techniques	119:139	extraction techniques	119:139	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	28	theme	polysaccharides	239:253	arg1	activity					219:226	antihyperglycemic activity	201:226	antihyperglycemic activity	201:226	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	28	theme	polysaccharides	239:253	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	28	theme	polysaccharides	239:253	arg1	activity					188:195	antioxidant activity	176:195	antioxidant activity	176:195	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	0	29	theme	comfrey	45:51	arg1	polysaccharides					53:67	comfrey polysaccharides	45:67	comfrey polysaccharides	45:67	Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques.					
29727640	5	30	theme	antioxidant	712:722	arg1	activities					724:733	antioxidant activities	712:733	antioxidant activities	712:733	CPs showed antioxidant activities and antihyperglycemic activities in a concentration-dependent manner.					
29727640	6	31	theme	molecular	896:904	arg1	weight					906:911	its smaller molecular weight	884:911	its smaller molecular weight	884:911	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	6	32	theme	smaller	888:894	arg1	weight					906:911	its smaller molecular weight	884:911	its smaller molecular weight	884:911	UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content.					
29727640	3	33	theme	Experimental	464:475	arg1	results					477:483	Experimental results	464:483	Experimental results	464:483	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	8	34	theme	higher	1174:1179	arg1	polysaccharides					1191:1205	higher bioactive polysaccharides	1174:1205	higher bioactive polysaccharides from comfrey	1174:1218	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	3	35	theme	higher	563:568	arg1	yields					581:586	higher extraction yields	563:586	higher extraction yields	563:586	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	1	36	theme	techniques	130:139	arg1	effects					108:114	The effects	104:114	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs)	104:259	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	0	37	theme	polysaccharides	53:67	arg1	activities					31:40	activities	31:40	activities	31:40	Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques.					
29727640	0	37	theme	polysaccharides	53:67	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques.					
29727640	3	38	theme	extraction	570:579	arg1	yields					581:586	higher extraction yields	563:586	higher extraction yields	563:586	Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields.					
29727640	8	39	from	comfrey	1212:1218	arg1	polysaccharides					1191:1205	higher bioactive polysaccharides	1174:1205	higher bioactive polysaccharides from comfrey	1174:1218	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	8	40	theme	extraction	1064:1073	arg1	technology					1075:1084	ultrasonic-assisted extraction technology	1044:1084	ultrasonic-assisted extraction technology	1044:1084	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	8	41	theme	bioactive	1181:1189	arg1	polysaccharides					1191:1205	higher bioactive polysaccharides	1174:1205	higher bioactive polysaccharides from comfrey	1174:1218	These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey.					
29727640	1	42	theme	physicochemical	148:162	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	0	43	theme	different	82:90	arg1	techniques					92:101	different techniques	82:101	different techniques	82:101	Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques.					
29727640	4	44	theme	monosaccharide	618:631	arg1	composition					633:643	the same monosaccharide composition	609:643	the same monosaccharide composition	609:643	The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content.					
29727640	1	45	theme	comfrey	231:237	arg1	polysaccharides					239:253	comfrey polysaccharides	231:253	comfrey polysaccharides (CPs)	231:259	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	45	theme	comfrey	231:237	arg1	CPs					256:258	CPs	256:258	CPs	256:258	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	4	46	theme	monosaccharide	677:690	arg1	content					692:698	monosaccharide content	677:698	monosaccharide content	677:698	The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content.					
29727640	1	47	from	effects	108:114	arg1	activity					219:226	antihyperglycemic activity	201:226	antihyperglycemic activity	201:226	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	47	from	effects	108:114	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	1	47	from	effects	108:114	arg1	activity					188:195	antioxidant activity	176:195	antioxidant activity	176:195	The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated.					
29727640	5	48	theme	concentration-dependent	773:795	arg1	manner					797:802	a concentration-dependent manner	771:802	a concentration-dependent manner	771:802	CPs showed antioxidant activities and antihyperglycemic activities in a concentration-dependent manner.					
29727640	4	49	theme	same	613:616	arg1	composition					633:643	the same monosaccharide composition	609:643	the same monosaccharide composition	609:643	The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content.					
30531805	7	0	theme	dihedral	1215:1222	arg1	angles					1224:1229	dihedral angles	1215:1229	dihedral angles within the pyrophosphate moiety	1215:1261	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
30531805	8	1	theme	chain	1474:1478	arg1	rearrangement					1446:1458	structural rearrangement	1435:1458	structural rearrangement of the glycan chain	1435:1478	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	5	2	theme	protein	858:864	arg1	PBP1b					870:874	PBP1b	870:874	PBP1b	870:874	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	5	2	theme	protein	858:864	arg1	1b					866:867	penicillin-binding protein 1b	839:867	penicillin-binding protein 1b (PBP1b) from Escherichia coli	839:897	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	1	3	theme	bacterial	155:163	arg1	formation					175:183	bacterial cell wall formation	155:183	bacterial cell wall formation	155:183	Peptidoglycan (PG) biosynthesis and assembly are needed for bacterial cell wall formation.					
30531805	4	4	theme	elongated	563:571	arg1	forms					573:577	its elongated forms	559:577	its elongated forms (lipid VI and lipid XII)	559:602	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	4	4	theme	elongated	563:571	arg1	lipid					580:584	lipid VI and lipid XII	580:601	lipid	580:584	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	4	4	theme	elongated	563:571	arg1	lipid					593:597	lipid	593:597	lipid	593:597	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	6	5	theme	non-reducing	1056:1067	arg1	end					1069:1071	the non-reducing end	1052:1071	the non-reducing end of the nascent PG	1052:1089	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	1	6	theme	cell	165:168	arg1	formation					175:183	bacterial cell wall formation	155:183	bacterial cell wall formation	155:183	Peptidoglycan (PG) biosynthesis and assembly are needed for bacterial cell wall formation.					
30531805	0	7	from	Insight	0:6	arg1	Membranes					84:92	Bacterial Cytoplasmic Membranes	62:92	Bacterial Cytoplasmic Membranes	62:92	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	5	8	from	simulated	915:923	arg1	presence					932:939	the presence	928:939	the presence of a nascent PG to investigate their interactions	928:989	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	4	9	theme	prototypical	761:772	arg1	composition					774:784	the prototypical composition	757:784	the prototypical composition of Gram-negative cytoplasmic membranes	757:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	6	10	theme	normal	1144:1149	arg1	membrane					1135:1142	the membrane	1131:1142	the membrane normal	1131:1149	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	2	11	contain	carries	247:253	arg1	precursor					202:210	the precursor	198:210	the precursor in the PG biosynthetic pathway	198:241	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	2	11	contain	carries	247:253	arg1	Lipid					186:190	Lipid II	186:193	Lipid II	186:193	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	2	11	contain	carries	247:253	arg2	unit					268:271	a nascent PG unit	255:271	a nascent PG unit that is processed by glycosyltransferases	255:313	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	9	12	theme	transpeptidase	1552:1565	arg1	domain					1567:1572	the transpeptidase domain	1548:1572	the transpeptidase domain	1548:1572	Most importantly, the number of nascent PG units required to reach the transpeptidase domain are determined to be 7 or 8.					
30531805	6	13	theme	membrane	1184:1191	arg1	surface					1193:1199	the membrane surface	1180:1199	the membrane surface	1180:1199	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	3	14	theme	membrane-anchored	490:506	arg1	enzymes					508:514	membrane-anchored enzymes	490:514	membrane-anchored enzymes	490:514	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	4	15	from	bilayers	634:641	arg1	modeled					609:615	modeled	609:615	modeled	609:615	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	6	16	theme	Trajectory	992:1001	arg1	analysis					1003:1010	Trajectory analysis	992:1010	Trajectory analysis	992:1010	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	2	17	from	precursor	202:210	arg1	pathway					235:241	the PG biosynthetic pathway	215:241	the PG biosynthetic pathway	215:241	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	2	18	theme	PG	265:266	arg1	unit					268:271	a nascent PG unit	255:271	a nascent PG unit that is processed by glycosyltransferases	255:313	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	1	19	theme	wall	170:173	arg1	formation					175:183	bacterial cell wall formation	155:183	bacterial cell wall formation	155:183	Peptidoglycan (PG) biosynthesis and assembly are needed for bacterial cell wall formation.					
30531805	0	20	theme	Processing	48:57	arg1	Stages					24:29	Elongation Stages	13:29	Elongation Stages of Peptidoglycan Processing	13:57	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	4	21	from	work	540:543	arg1	modeled					609:615	modeled	609:615	modeled	609:615	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	5	22	from	presence	932:939	arg1	simulated					915:923	simulated	915:923	simulated	915:923	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	2	23	theme	nascent	257:263	arg1	unit					268:271	a nascent PG unit	255:271	a nascent PG unit that is processed by glycosyltransferases	255:313	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	3	24	theme	lipid	432:436	arg1	interactions					472:483	its interactions	468:483	its interactions with membrane-anchored enzymes	468:514	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	24	theme	lipid	432:436	arg1	ensemble					420:427	the conformational ensemble	401:427	the conformational ensemble of lipid II in bacterial membranes	401:462	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	25	theme	several	369:375	arg1	classes					377:383	several classes	369:383	several classes of antibiotics	369:398	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	26	with	interactions	472:483	arg1	enzymes					508:514	membrane-anchored enzymes	490:514	membrane-anchored enzymes	490:514	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	10	27	theme	scaffold	1737:1744	arg1	assembly					1718:1725	the assembly	1714:1725	the assembly of the PG scaffold	1714:1744	Our findings complement experimental results to further understand how the structure of nascent PG can dictate the assembly of the PG scaffold.					
30531805	1	28	theme	Peptidoglycan	95:107	arg1	biosynthesis					114:125	Peptidoglycan (PG) biosynthesis	95:125	Peptidoglycan (PG) biosynthesis	95:125	Peptidoglycan (PG) biosynthesis and assembly are needed for bacterial cell wall formation.					
30531805	3	29	theme	conformational	405:418	arg1	ensemble					420:427	the conformational ensemble	401:427	the conformational ensemble of lipid II in bacterial membranes	401:462	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	30	theme	classes	377:383	arg1	target					359:364	a target	357:364	a target of several classes of antibiotics	357:398	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	4	31	theme	lipid	546:550	arg1	II					552:553	lipid II	546:553	lipid II	546:553	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	3	32	from	interactions	472:483	arg1	membranes					454:462	bacterial membranes	444:462	bacterial membranes	444:462	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	5	33	theme	nascent	946:952	arg1	PG					954:955	a nascent PG	944:955	a nascent PG	944:955	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	4	34	from	modeled	609:615	arg1	work					540:543	this work	535:543	this work	535:543	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	4	34	from	modeled	609:615	arg1	bilayers					634:641	bilayers	634:641	bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes	634:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	5	35	from	coli	894:897	arg1	PBP1b					870:874	PBP1b	870:874	PBP1b	870:874	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	5	35	from	coli	894:897	arg1	1b					866:867	penicillin-binding protein 1b	839:867	penicillin-binding protein 1b (PBP1b) from Escherichia coli	839:897	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	1	36	theme	PG	110:111	arg1	biosynthesis					114:125	Peptidoglycan (PG) biosynthesis	95:125	Peptidoglycan (PG) biosynthesis	95:125	Peptidoglycan (PG) biosynthesis and assembly are needed for bacterial cell wall formation.					
30531805	3	37	theme	bacterial	444:452	arg1	membranes					454:462	bacterial membranes	444:462	bacterial membranes	444:462	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	0	38	theme	Elongation	13:22	arg1	Stages					24:29	Elongation Stages	13:29	Elongation Stages of Peptidoglycan Processing	13:57	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	9	39	theme	units	1524:1528	arg1	7					1595:1595	7	1595:1595	7	1595:1595	Most importantly, the number of nascent PG units required to reach the transpeptidase domain are determined to be 7 or 8.					
30531805	9	39	theme	units	1524:1528	arg1	number					1503:1508	the number	1499:1508	the number of nascent PG units required to reach the transpeptidase domain	1499:1572	Most importantly, the number of nascent PG units required to reach the transpeptidase domain are determined to be 7 or 8.					
30531805	3	40	theme	therapeutic	336:346	arg1	value					348:352	its immense therapeutic value	324:352	its immense therapeutic value as a target of several classes of antibiotics	324:398	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	6	41	theme	PG	1088:1089	arg1	end					1069:1071	the non-reducing end	1052:1071	the non-reducing end of the nascent PG	1052:1089	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	6	42	theme	greater	1105:1111	arg1	fluctuation					1113:1123	much greater fluctuation	1100:1123	much greater fluctuation along the membrane normal	1100:1149	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	0	43	theme	Peptidoglycan	34:46	arg1	Processing					48:57	Peptidoglycan Processing	34:57	Peptidoglycan Processing	34:57	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	10	44	theme	PG	1734:1735	arg1	scaffold					1737:1744	the PG scaffold	1730:1744	the PG scaffold	1730:1744	Our findings complement experimental results to further understand how the structure of nascent PG can dictate the assembly of the PG scaffold.					
30531805	2	45	theme	biosynthetic	222:233	arg1	pathway					235:241	the PG biosynthetic pathway	215:241	the PG biosynthetic pathway	215:241	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	7	46	theme	surrounding	1317:1327	arg1	environment					1329:1339	its surrounding environment	1313:1339	its surrounding environment	1313:1339	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
30531805	6	47	theme	nascent	1080:1086	arg1	PG					1088:1089	the nascent PG	1076:1089	the nascent PG	1076:1089	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	8	48	with	contact	1413:1419	arg1	PBP1b					1426:1430	PBP1b	1426:1430	PBP1b	1426:1430	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	7	49	theme	environment	1329:1339	arg1	length					1285:1290	the length	1281:1290	the length of the PG moiety and its surrounding environment	1281:1339	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
30531805	2	50	theme	PG	219:220	arg1	pathway					235:241	the PG biosynthetic pathway	215:241	the PG biosynthetic pathway	215:241	Lipid II is the precursor in the PG biosynthetic pathway and carries a nascent PG unit that is processed by glycosyltransferases.					
30531805	7	51	theme	PG	1299:1300	arg1	moiety					1302:1307	the PG moiety	1295:1307	the PG moiety	1295:1307	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
30531805	5	52	theme	penicillin-binding	839:856	arg1	PBP1b					870:874	PBP1b	870:874	PBP1b	870:874	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	5	52	theme	penicillin-binding	839:856	arg1	1b					866:867	penicillin-binding protein 1b	839:867	penicillin-binding protein 1b (PBP1b) from Escherichia coli	839:897	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	3	53	theme	immense	328:334	arg1	value					348:352	its immense therapeutic value	324:352	its immense therapeutic value as a target of several classes of antibiotics	324:398	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	54	theme	antibiotics	388:398	arg1	classes					377:383	several classes	369:383	several classes of antibiotics	369:398	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	3	55	with	ensemble	420:427	arg1	enzymes					508:514	membrane-anchored enzymes	490:514	membrane-anchored enzymes	490:514	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	9	56	theme	nascent	1513:1519	arg1	units					1524:1528	nascent PG units	1513:1528	nascent PG units	1513:1528	Most importantly, the number of nascent PG units required to reach the transpeptidase domain are determined to be 7 or 8.					
30531805	8	57	theme	stem	1383:1386	arg1	peptide					1388:1394	the stem peptide	1379:1394	the stem peptide	1379:1394	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	8	58	theme	structural	1435:1444	arg1	rearrangement					1446:1458	structural rearrangement	1435:1458	structural rearrangement of the glycan chain	1435:1478	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	8	59	theme	close	1407:1411	arg1	contact					1413:1419	close contact	1407:1419	close contact with PBP1b by structural rearrangement of the glycan chain	1407:1478	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	4	60	theme	cytoplasmic	803:813	arg1	membranes					815:823	Gram-negative cytoplasmic membranes	789:823	Gram-negative cytoplasmic membranes	789:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	5	61	theme	PG	954:955	arg1	presence					932:939	the presence	928:939	the presence of a nascent PG to investigate their interactions	928:989	In addition, penicillin-binding protein 1b (PBP1b) from Escherichia coli was modeled and simulated in the presence of a nascent PG to investigate their interactions.					
30531805	10	62	theme	nascent	1691:1697	arg1	PG					1699:1700	nascent PG	1691:1700	nascent PG	1691:1700	Our findings complement experimental results to further understand how the structure of nascent PG can dictate the assembly of the PG scaffold.					
30531805	8	63	theme	nascent	1349:1355	arg1	PG					1357:1358	a nascent PG	1347:1358	a nascent PG	1347:1358	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	8	64	theme	glycan	1467:1472	arg1	chain					1474:1478	the glycan chain	1463:1478	the glycan chain	1463:1478	When a nascent PG is bound to PBP1b, the stem peptide remains in close contact with PBP1b by structural rearrangement of the glycan chain.					
30531805	4	65	theme	Gram-negative	789:801	arg1	membranes					815:823	Gram-negative cytoplasmic membranes	789:823	Gram-negative cytoplasmic membranes	789:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	10	66	theme	experimental	1627:1638	arg1	results					1640:1646	experimental results	1627:1646	experimental results	1627:1646	Our findings complement experimental results to further understand how the structure of nascent PG can dictate the assembly of the PG scaffold.					
30531805	4	67	theme	POPG	700:703	arg1	bilayers					634:641	bilayers	634:641	bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes	634:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	6	68	theme	glycan	1032:1037	arg1	chain					1039:1043	the glycan chain	1028:1043	the glycan chain	1028:1043	Trajectory analysis reveals that as the glycan chain grows, the non-reducing end of the nascent PG displays much greater fluctuation along the membrane normal and minimally interacts with the membrane surface.					
30531805	0	69	theme	Cytoplasmic	72:82	arg1	Membranes					84:92	Bacterial Cytoplasmic Membranes	62:92	Bacterial Cytoplasmic Membranes	62:92	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	4	70	dep	lipid	580:584	arg1	VI					586:587	VI	586:587	VI	586:587	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	4	70	dep	lipid	580:584	arg1	XII					599:601	XII	599:601	XII	599:601	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	3	71	from	ensemble	420:427	arg1	membranes					454:462	bacterial membranes	444:462	bacterial membranes	444:462	Despite its immense therapeutic value as a target of several classes of antibiotics, the conformational ensemble of lipid II in bacterial membranes and its interactions with membrane-anchored enzymes remain elusive.					
30531805	4	72	theme	POPE	646:649	arg1	bilayers					634:641	bilayers	634:641	bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes	634:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	7	73	theme	moiety	1302:1307	arg1	length					1285:1290	the length	1281:1290	the length of the PG moiety and its surrounding environment	1281:1339	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
30531805	9	74	theme	PG	1521:1522	arg1	units					1524:1528	nascent PG units	1513:1528	nascent PG units	1513:1528	Most importantly, the number of nascent PG units required to reach the transpeptidase domain are determined to be 7 or 8.					
30531805	0	75	theme	Bacterial	62:70	arg1	Membranes					84:92	Bacterial Cytoplasmic Membranes	62:92	Bacterial Cytoplasmic Membranes	62:92	Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.					
30531805	4	76	theme	membranes	815:823	arg1	composition					774:784	the prototypical composition	757:784	the prototypical composition of Gram-negative cytoplasmic membranes	757:823	In this work, lipid II and its elongated forms (lipid VI and lipid XII) were modeled and simulated in bilayers of POPE (palmitoyl-oleoyl-phosphatidyl-ethanolamine) and POPG (palmitoyl-oleoyl-phosphatidyl-glycerol) that mimic the prototypical composition of Gram-negative cytoplasmic membranes.					
30531805	10	77	theme	PG	1699:1700	arg1	structure					1678:1686	the structure	1674:1686	the structure of nascent PG	1674:1700	Our findings complement experimental results to further understand how the structure of nascent PG can dictate the assembly of the PG scaffold.					
30531805	7	78	theme	pyrophosphate	1242:1254	arg1	moiety					1256:1261	the pyrophosphate moiety	1238:1261	the pyrophosphate moiety	1238:1261	In addition, dihedral angles within the pyrophosphate moiety are determined by the length of the PG moiety and its surrounding environment.					
31888838	4	0	theme	NMR	324:326	arg1	spectroscopy					328:339	2D NMR spectroscopy	321:339	2D NMR spectroscopy	321:339	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	7	1	theme	partial	969:975	arg1	CH50					989:992	CH50	989:992	CH50: 197.76 ± 21.81 μg/mL	989:1014	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	7	1	theme	partial	969:975	arg1	hydrolysis					977:986	partial hydrolysis	969:986	partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL)	969:1015	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	2	2	theme	acidic	128:133	arg1	polysaccharide					135:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	2	2	theme	acidic	128:133	arg1	XB-PS3					151:156	XB-PS3	151:156	XB-PS3	151:156	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	3	3	theme	molecular	228:236	arg1	weight					238:243	a molecular weight	226:243	a molecular weight of 86.04 kDa	226:256	Wilsonii with a molecular weight of 86.04 kDa.					
31888838	2	4	theme	water-soluble	114:126	arg1	polysaccharide					135:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	2	4	theme	water-soluble	114:126	arg1	XB-PS3					151:156	XB-PS3	151:156	XB-PS3	151:156	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	7	5	theme	important	1033:1041	arg1	role					1043:1046	the important role	1029:1046	the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity	1029:1131	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	7	6	dep	reduced	900:906	arg1	CH50					909:912	CH50	909:912	CH50: 268.55 ± 16.82 μg/mL	909:934	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	1	7	theme	Extraction	54:63	arg1	activity					102:109	Extraction, characterization and anticomplement activity	54:109	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	7	8	theme	anticomplement	1109:1122	arg1	activity					1124:1131	the polysaccharide's anticomplement activity	1088:1131	the polysaccharide's anticomplement activity	1088:1131	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	3	9	theme	86.04 kDa	248:256	arg1	weight					238:243	a molecular weight	226:243	a molecular weight of 86.04 kDa	226:256	Wilsonii with a molecular weight of 86.04 kDa.					
31888838	1	10	theme	characterization	66:81	arg1	activity					102:109	Extraction, characterization and anticomplement activity	54:109	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	4	11	theme	composition	286:296	arg1	analysis					298:305	monosaccharide composition analysis	271:305	monosaccharide composition analysis	271:305	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	5	12	theme	possible	534:541	arg1	units					553:557	The possible repeating units	530:557	The possible repeating units	530:557	The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis.					
31888838	0	13	theme	pingii	10:15	arg1	var					17:19	Juniperus pingii var	0:19	Juniperus pingii var.	0:20	Juniperus pingii var.					
31888838	5	14	theme	repeating	543:551	arg1	units					553:557	The possible repeating units	530:557	The possible repeating units	530:557	The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis.					
31888838	7	15	theme	galacturonic	876:887	arg1	acids					889:893	the galacturonic acids	872:893	the galacturonic acids	872:893	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	1	16	theme	anticomplement	87:100	arg1	activity					102:109	Extraction, characterization and anticomplement activity	54:109	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	0	17	theme	Juniperus	0:8	arg1	var					17:19	Juniperus pingii var	0:19	Juniperus pingii var.	0:20	Juniperus pingii var.					
31888838	7	18	dep	CH50	909:912	arg1	268.55 ± 16.82 μg/mL					915:934	268.55 ± 16.82 μg/mL	915:934	CH50: 268.55 ± 16.82 μg/mL	909:934	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	6	19	from	C4	751:752	arg1	cascade					794:800	the complement activation cascade	768:800	the complement activation cascade	768:800	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	4	20	theme	araban	479:484	arg1	branch					486:491	an araban branch	476:491	an araban branch attached to O-2 of →2,4)-α-Manp-(1→	476:527	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	5	21	theme	acid	622:625	arg1	hydrolysis					627:636	partial acid hydrolysis	614:636	partial acid hydrolysis	614:636	The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis.					
31888838	6	22	theme	117.23 ± 18.74 μg/mL	706:725	arg1	value					697:701	CH50 value	692:701	CH50 value of 117.23 ± 18.74 μg/mL	692:725	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	1	23	dep	polysaccharide	38:51	arg1	activity					102:109	Extraction, characterization and anticomplement activity	54:109	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	4	24	dep	-α-GalpA-	439:447	arg1	esterified					458:467	esterified	458:467	esterified	458:467	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	25	attach	attached	493:500	arg2	branch					486:491	an araban branch	476:491	an araban branch attached to O-2 of →2,4)-α-Manp-(1→	476:527	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	25	attach	attached	493:500	arg1	O-2					505:507	O-2	505:507	O-2 of →2,4)-α-Manp-(1→	505:527	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	26	theme	monosaccharide	271:284	arg1	analysis					298:305	monosaccharide composition analysis	271:305	monosaccharide composition analysis	271:305	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	7	27	theme	branch	1068:1073	arg1	structure					1075:1083	branch structure	1068:1083	branch structure	1068:1083	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	6	28	theme	activation	783:792	arg1	cascade					794:800	the complement activation cascade	768:800	the complement activation cascade	768:800	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	6	29	from	C5	755:756	arg1	cascade					794:800	the complement activation cascade	768:800	the complement activation cascade	768:800	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	4	30	dep	esterified	458:467	arg1	1→					449:450	1→	449:450	1→	449:450	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	30	dep	esterified	458:467	arg1	%					456:456	60 %	453:456	60 %	453:456	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	7	31	theme	structure	1075:1083	arg1	role					1043:1046	the important role	1029:1046	the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity	1029:1131	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	6	32	theme	complement	772:781	arg1	cascade					794:800	the complement activation cascade	768:800	the complement activation cascade	768:800	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	4	33	theme	UPLC-MS	345:351	arg1	analysis					353:360	UPLC-MS analysis	345:360	UPLC-MS analysis	345:360	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	34	contain	had	388:390	arg1	XB-PS3					381:386	XB-PS3	381:386	XB-PS3	381:386	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	34	contain	had	388:390	arg2	backbone					394:401	a backbone	392:401	a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified)	392:468	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	7	35	from	role	1043:1046	arg1	activity					1124:1131	the polysaccharide's anticomplement activity	1088:1131	the polysaccharide's anticomplement activity	1088:1131	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	5	36	theme	oligosaccharide	585:599	arg1	analysis					601:608	oligosaccharide analysis	585:608	oligosaccharide analysis	585:608	The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis.					
31888838	7	37	dep	CH50	989:992	arg1	197.76 ± 21.81 μg/mL					995:1014	197.76 ± 21.81 μg/mL	995:1014	CH50: 197.76 ± 21.81 μg/mL	989:1014	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	6	38	from	C9	762:763	arg1	cascade					794:800	the complement activation cascade	768:800	the complement activation cascade	768:800	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	7	39	theme	uronic	1051:1056	arg1	acids					1058:1062	uronic acids	1051:1062	uronic acids	1051:1062	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	6	40	theme	CH50	692:695	arg1	value					697:701	CH50 value	692:701	CH50 value of 117.23 ± 18.74 μg/mL	692:725	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	2	41	attach	isolated	163:170	arg1	twigs					181:185	the twigs	177:185	the twigs of Juniperus pingii var	177:209	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	2	41	attach	isolated	163:170	arg2	XB-PS3					151:156	XB-PS3	151:156	XB-PS3	151:156	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	2	41	attach	isolated	163:170	arg2	polysaccharide					135:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide	112:148	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	2	42	theme	var	207:209	arg1	twigs					181:185	the twigs	177:185	the twigs of Juniperus pingii var	177:209	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	1	43	theme	wilsonii	22:29	arg1	polysaccharide					38:51	wilsonii acidic polysaccharide	22:51	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	4	44	theme	-α-Manp-	420:427	arg1	-α-GalpA-					439:447	→2,4)-α-Manp-(1→ and →4)-α-GalpA-	415:447	→2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified)	415:468	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	45	dep	-α-Manp-	420:427	arg1	1→					429:430	1→	429:430	1→	429:430	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	45	dep	-α-Manp-	420:427	arg1	→4					436:437	→4	436:437	→4	436:437	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	5	46	theme	partial	614:620	arg1	hydrolysis					627:636	partial acid hydrolysis	614:636	partial acid hydrolysis	614:636	The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis.					
31888838	2	47	theme	pingii	200:205	arg1	var					207:209	Juniperus pingii var	190:209	Juniperus pingii var	190:209	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	1	48	theme	acidic	31:36	arg1	polysaccharide					38:51	wilsonii acidic polysaccharide	22:51	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.	22:110	wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity.					
31888838	4	49	theme	2D	321:322	arg1	spectroscopy					328:339	2D NMR spectroscopy	321:339	2D NMR spectroscopy	321:339	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	6	50	theme	potent	656:661	arg1	activity					678:685	potent anticomplement activity	656:685	potent anticomplement activity	656:685	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	7	51	theme	anticomplement	816:829	arg1	activity					831:838	the anticomplement activity	812:838	the anticomplement activity	812:838	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	4	52	theme	-α-Manp-	517:524	arg1	1→					526:527	→2,4)-α-Manp-(1→	512:527	→2,4)-α-Manp-(1→	512:527	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	2	53	theme	Juniperus	190:198	arg1	var					207:209	Juniperus pingii var	190:209	Juniperus pingii var	190:209	A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var.					
31888838	6	54	theme	anticomplement	663:676	arg1	activity					678:685	potent anticomplement activity	656:685	potent anticomplement activity	656:685	XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 μg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade.					
31888838	7	55	theme	acids	1058:1062	arg1	role					1043:1046	the important role	1029:1046	the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity	1029:1131	However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 μg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 μg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.					
31888838	4	56	dep	analysis	298:305	arg1	means					262:266	means	262:266	means	262:266	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31888838	4	57	theme	1→	526:527	arg1	O-2					505:507	O-2	505:507	O-2 of →2,4)-α-Manp-(1→	505:527	By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→.					
31287456	8	0	theme	treatment	1424:1432	arg1	combination					1386:1396	a combination	1384:1396	a combination of chemical and enzymatic treatment	1384:1432	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	4	1	theme	chromatography-mass	673:691	arg1	LC-MS					707:711	LC-MS	707:711	LC-MS	707:711	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	4	1	theme	chromatography-mass	673:691	arg1	spectrometry					693:704	liquid chromatography-mass spectrometry	666:704	liquid chromatography-mass spectrometry (LC-MS)	666:712	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	7	2	theme	polysaccharide	1169:1182	arg1	lyases					1184:1189	polysaccharide lyases	1169:1189	polysaccharide lyases	1169:1189	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	5	3	theme	low	859:861	arg1	heparin					880:886	low molecular weight heparin	859:886	low molecular weight heparin	859:886	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	2	4	theme	interstitial	299:310	arg1	cystitis					312:319	interstitial cystitis	299:319	interstitial cystitis	299:319	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	5	5	dep	6000	831:834	arg1	to					828:829	to	828:829	to	828:829	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	8	6	theme	GAG	1354:1356	arg1	analysis					1358:1365	GAG analysis	1354:1365	GAG analysis	1354:1365	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	1	7	theme	Pentosan	58:65	arg1	glycosaminoglycan					105:121	a semi-synthetic glycosaminoglycan	88:121	a semi-synthetic glycosaminoglycan (GAG) mimetic	88:135	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	1	7	theme	Pentosan	58:65	arg1	PPS					80:82	PPS	80:82	PPS	80:82	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	1	7	theme	Pentosan	58:65	arg1	polysulfate					67:77	Pentosan polysulfate	58:77	Pentosan polysulfate (PPS)	58:83	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	4	8	theme	bottom-up	724:732	arg1	information					734:744	bottom-up information	724:744	bottom-up information on its composition	724:763	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	2	9	theme	active	231:236	arg1	PPS					138:140	PPS	138:140	PPS	138:140	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	9	theme	active	231:236	arg1	ingredient					253:262	the active pharmaceutical ingredient	227:262	the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis	227:319	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	3	10	theme	polyanionic	436:446	arg1	polysaccharide					448:461	an unnatural polyanionic polysaccharide	423:461	an unnatural polyanionic polysaccharide	423:461	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	3	10	theme	polyanionic	436:446	arg1	PPS					416:418	PPS	416:418	PPS	416:418	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	4	11	theme	liquid	666:671	arg1	LC-MS					707:711	LC-MS	707:711	LC-MS	707:711	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	4	11	theme	liquid	666:671	arg1	spectrometry					693:704	liquid chromatography-mass spectrometry	666:704	liquid chromatography-mass spectrometry (LC-MS)	666:712	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	6	12	theme	structural	1084:1093	arg1	information					1095:1105	the first detailed compositional and structural information	1047:1105	the first detailed compositional and structural information on PPS	1047:1112	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	7	13	theme	chondroitin	1252:1262	arg1	sulfates					1264:1271	chondroitin sulfates	1252:1271	chondroitin sulfates	1252:1271	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	6	14	theme	top-down	1013:1020	arg1	analysis					1022:1029	The bottom-up and top-down analysis	995:1029	analysis	1022:1029	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	7	15	theme	heparan	1274:1280	arg1	sulfate					1282:1288	heparan sulfate	1274:1288	heparan sulfate	1274:1288	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	5	16	theme	molecular	863:871	arg1	heparin					880:886	low molecular weight heparin	859:886	low molecular weight heparin	859:886	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	4	17	from	information	734:744	arg1	composition					753:763	its composition	749:763	its composition	749:763	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	8	18	used	used	1443:1446	arg2	combination					1386:1396	a combination	1384:1396	a combination of chemical and enzymatic treatment	1384:1432	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	5	19	from	LC-MS	937:941	arg1	chains					957:962	its intact chains	946:962	its intact chains	946:962	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	4	20	theme	oligosaccharide	618:632	arg1	fragments					634:642	the resulting oligosaccharide fragments	604:642	the resulting oligosaccharide fragments	604:642	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	2	21	theme	pharmaceutical	238:251	arg1	PPS					138:140	PPS	138:140	PPS	138:140	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	21	theme	pharmaceutical	238:251	arg1	ingredient					253:262	the active pharmaceutical ingredient	227:262	the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis	227:319	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	7	22	theme	GAGs	1239:1242	arg1	analysis					1219:1226	the analysis	1215:1226	the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates	1215:1310	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	2	23	theme	drug	271:274	arg1	Elmiron™					276:283	the drug Elmiron™	267:283	the drug Elmiron™ used to treat interstitial cystitis	267:319	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	6	24	theme	PPS	1034:1036	arg1	analysis					1022:1029	The bottom-up and top-down analysis	995:1029	analysis	1022:1029	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	5	25	theme	similar	840:846	arg1	weight					803:808	an average molecular weight	782:808	an average molecular weight	782:808	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	5	26	theme	molecular	793:801	arg1	weight					803:808	an average molecular weight	782:808	an average molecular weight	782:808	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	8	27	theme	natural	1483:1489	arg1	GAGs					1491:1494	natural GAGs	1483:1494	natural GAGs	1483:1494	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	8	28	contain	containing	1467:1476	arg1	samples					1459:1465	samples	1459:1465	samples containing both natural GAGs and PPS	1459:1502	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	8	28	contain	containing	1467:1476	arg2	PPS					1500:1502	PPS	1500:1502	PPS	1500:1502	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	8	28	contain	containing	1467:1476	arg2	GAGs					1491:1494	natural GAGs	1483:1494	natural GAGs	1483:1494	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	3	29	theme	natural	329:335	arg1	GAGs					337:340	natural GAGs	329:340	natural GAGs that can be enzymatically broken down into oligosaccharides for analysis	329:413	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	5	30	theme	top-down	976:983	arg1	analysis					985:992	top-down analysis	976:992	top-down analysis	976:992	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	6	31	from	information	1095:1105	arg1	PPS					1110:1112	PPS	1110:1112	PPS	1110:1112	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	6	32	theme	bottom-up	999:1007	arg1	analysis					1022:1029	The bottom-up and top-down analysis	995:1029	analysis	1022:1029	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	0	33	theme	top-down	14:21	arg1	profiling					23:31	top-down profiling	14:31	top-down profiling	14:31	Bottom-up and top-down profiling of pentosan polysulfate.					
31287456	4	34	theme	oxygen	532:537	arg1	species					539:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	2	35	theme	chemical	167:174	arg1	sulfonation					176:186	the chemical sulfonation	163:186	the chemical sulfonation of a plant-derived β-(1 → 4)-xylan	163:221	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	1	36	theme	semi-synthetic	90:103	arg1	glycosaminoglycan					105:121	a semi-synthetic glycosaminoglycan	88:121	a semi-synthetic glycosaminoglycan (GAG) mimetic	88:135	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	1	36	theme	semi-synthetic	90:103	arg1	polysulfate					67:77	Pentosan polysulfate	58:77	Pentosan polysulfate (PPS)	58:83	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	4	37	theme	PPS	596:598	arg1	depolymerization					576:591	the controlled depolymerization	561:591	the controlled depolymerization of PPS	561:598	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	0	38	theme	pentosan	36:43	arg1	polysulfate					45:55	pentosan polysulfate	36:55	pentosan polysulfate	36:55	Bottom-up and top-down profiling of pentosan polysulfate.					
31287456	4	39	theme	reactive	523:530	arg1	species					539:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	4	40	theme	resulting	608:616	arg1	fragments					634:642	the resulting oligosaccharide fragments	604:642	the resulting oligosaccharide fragments	604:642	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	2	41	link	plant-derived	193:205	arg1	β-					207:208	a plant-derived β-	191:208	a plant-derived β-(1 → 4)-xylan	191:221	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	41	link	plant-derived	193:205	arg1	→					212:212	1 → 4	210:214	1 → 4	210:214	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	8	42	theme	chemical	1401:1408	arg1	treatment					1424:1432	chemical and enzymatic treatment	1401:1432	chemical and enzymatic treatment	1401:1432	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	4	43	used	used	552:555	arg2	species					539:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species	515:545	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	6	44	theme	first	1051:1055	arg1	information					1095:1105	the first detailed compositional and structural information	1047:1105	the first detailed compositional and structural information on PPS	1047:1112	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	5	45	theme	weight	873:878	arg1	heparin					880:886	low molecular weight heparin	859:886	low molecular weight heparin	859:886	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	0	46	theme	polysulfate	45:55	arg1	Bottom-up					0:8	Bottom-up	0:8	Bottom-up	0:8	Bottom-up and top-down profiling of pentosan polysulfate.					
31287456	0	46	theme	polysulfate	45:55	arg1	profiling					23:31	top-down profiling	14:31	top-down profiling	14:31	Bottom-up and top-down profiling of pentosan polysulfate.					
31287456	2	47	theme	plant-derived	193:205	arg1	β-					207:208	a plant-derived β-	191:208	a plant-derived β-(1 → 4)-xylan	191:221	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	47	theme	plant-derived	193:205	arg1	→					212:212	1 → 4	210:214	1 → 4	210:214	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	48	theme	β-	207:208	arg1	-xylan					216:221	a plant-derived β-(1 → 4)-xylan	191:221	a plant-derived β-(1 → 4)-xylan	191:221	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	7	49	theme	natural	1231:1237	arg1	GAGs					1239:1242	natural GAGs	1231:1242	natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates	1231:1310	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	7	49	theme	natural	1231:1237	arg1	sulfates					1303:1310	keratan sulfates	1295:1310	keratan sulfates	1295:1310	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	7	49	theme	natural	1231:1237	arg1	sulfates					1264:1271	chondroitin sulfates	1252:1271	chondroitin sulfates	1252:1271	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	7	49	theme	natural	1231:1237	arg1	sulfate					1282:1288	heparan sulfate	1274:1288	heparan sulfate	1274:1288	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	5	50	contain	has	778:780	arg2	weight					803:808	an average molecular weight	782:808	an average molecular weight	782:808	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	5	50	contain	has	778:780	arg1	PPS					774:776	PPS	774:776	PPS	774:776	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	7	51	theme	keratan	1295:1301	arg1	sulfates					1303:1310	keratan sulfates	1295:1310	keratan sulfates	1295:1310	Finally, we examined whether PPS would interfere with polysaccharide lyases and hydrolases, used in the analysis of natural GAGs such as chondroitin sulfates, heparan sulfate, and keratan sulfates.					
31287456	3	52	theme	unnatural	426:434	arg1	polysaccharide					448:461	an unnatural polyanionic polysaccharide	423:461	an unnatural polyanionic polysaccharide	423:461	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	3	52	theme	unnatural	426:434	arg1	PPS					416:418	PPS	416:418	PPS	416:418	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	6	53	theme	detailed	1057:1064	arg1	information					1095:1105	the first detailed compositional and structural information	1047:1105	the first detailed compositional and structural information on PPS	1047:1112	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	4	54	theme	controlled	565:574	arg1	depolymerization					576:591	the controlled depolymerization	561:591	the controlled depolymerization of PPS	561:598	Instead reactive oxygen species were used for the controlled depolymerization of PPS and the resulting oligosaccharide fragments were then analyzed by liquid chromatography-mass spectrometry (LC-MS) to obtain bottom-up information on its composition.					
31287456	8	55	theme	enzymatic	1414:1422	arg1	treatment					1424:1432	chemical and enzymatic treatment	1401:1432	chemical and enzymatic treatment	1401:1432	We found that PPS did not interfere with GAG analysis, suggesting that a combination of chemical and enzymatic treatment could be used to analyze samples containing both natural GAGs and PPS.					
31287456	2	56	theme	-xylan	216:221	arg1	sulfonation					176:186	the chemical sulfonation	163:186	the chemical sulfonation of a plant-derived β-(1 → 4)-xylan	163:221	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	57	theme	Elmiron™	276:283	arg1	PPS					138:140	PPS	138:140	PPS	138:140	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	2	57	theme	Elmiron™	276:283	arg1	ingredient					253:262	the active pharmaceutical ingredient	227:262	the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis	227:319	PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug Elmiron™ used to treat interstitial cystitis.					
31287456	6	58	theme	compositional	1066:1078	arg1	information					1095:1105	the first detailed compositional and structural information	1047:1105	the first detailed compositional and structural information on PPS	1047:1112	The bottom-up and top-down analysis of PPS provides the first detailed compositional and structural information on PPS.					
31287456	1	59	theme	mimetic	129:135	arg1	glycosaminoglycan					105:121	a semi-synthetic glycosaminoglycan	88:121	a semi-synthetic glycosaminoglycan (GAG) mimetic	88:135	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	1	59	theme	mimetic	129:135	arg1	polysulfate					67:77	Pentosan polysulfate	58:77	Pentosan polysulfate (PPS)	58:83	Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic.					
31287456	3	60	theme	analytical	494:503	arg1	approach					505:512	such an analytical approach	486:512	such an analytical approach	486:512	Unlike natural GAGs that can be enzymatically broken down into oligosaccharides for analysis, PPS is an unnatural polyanionic polysaccharide and is not amenable to such an analytical approach.					
31287456	5	61	theme	intact	950:955	arg1	chains					957:962	its intact chains	946:962	its intact chains	946:962	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
31287456	5	62	theme	average	785:791	arg1	weight					803:808	an average molecular weight	782:808	an average molecular weight	782:808	Because PPS has an average molecular weight ranging from 4000 to 6000 Da, similar to that of low molecular weight heparin, this suggested that it might be possible to use LC-MS on its intact chains and perform top-down analysis.					
30933826	4	0	theme	matter	639:644	arg1	content					646:652	volatile matter content	630:652	volatile matter content	630:652	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	8	1	theme	torrefied	981:989	arg1	char					991:994	torrefied char	981:994	torrefied char	981:994	The combustion kinetic of torrefied char were calculated using the Friedman method and the Ozawa-Flynn-Wall method.					
30933826	1	2	theme	routine	142:148	arg1	pyrolysis					150:158	the pyrolysis gas and heat from biomass routine pyrolysis	102:158	pyrolysis	150:158	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	8	3	theme	Friedman	1022:1029	arg1	method					1031:1036	the Friedman method	1018:1036	the Friedman method	1018:1036	The combustion kinetic of torrefied char were calculated using the Friedman method and the Ozawa-Flynn-Wall method.					
30933826	6	4	theme	Chemical	773:780	arg1	structure					782:790	Chemical structure	773:790	Chemical structure	773:790	Chemical structure is different for torrefied char under pyrolysis gas and N2 atmosphere.					
30933826	3	5	theme	composition	478:488	arg1	influence					460:468	the influence	456:468	the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance	456:561	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	3	6	from	CO2	502:504	arg1	gas					527:529	pyrolysis gas	517:529	pyrolysis gas	517:529	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	3	7	theme	pyrolysis	517:525	arg1	gas					527:529	pyrolysis gas	517:529	pyrolysis gas	517:529	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	4	8	dep	content	646:652	arg1	the					626:628	the	626:628	the	626:628	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	7	9	theme	synergy	907:913	arg1	role					915:918	synergy role	907:918	synergy role	907:918	And each composition in pyrolysis gas plays synergy role to the severity of torrefied char.					
30933826	2	10	theme	char	269:272	arg1	performance					364:374	combustion performance	353:374	combustion performance	353:374	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	10	theme	char	269:272	arg1	pyrolysis					339:347	pyrolysis	339:347	pyrolysis	339:347	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	10	theme	char	269:272	arg1	properties					245:254	And the chemical and physical properties	215:254	properties	245:254	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	6	11	theme	N2	848:849	arg1	atmosphere					851:860	pyrolysis gas and N2 atmosphere	830:860	atmosphere	851:860	Chemical structure is different for torrefied char under pyrolysis gas and N2 atmosphere.					
30933826	4	12	theme	char	686:689	arg1	content					646:652	volatile matter content	630:652	volatile matter content	630:652	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	4	12	theme	char	686:689	arg1	value					663:667	heat value	658:667	heat value	658:667	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	2	13	theme	torrefied	259:267	arg1	char					269:272	torrefied char	259:272	torrefied char obtained under N2 and pyrolysis gas	259:308	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	1	14	theme	camellia	165:172	arg1	shell					174:178	the camellia shell	161:178	the camellia shell	161:178	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	5	15	theme	cellulose	744:752	arg1	degradation					729:739	the degradation	725:739	the degradation of cellulose and hemicellulose	725:770	Moreover, pyrolysis gas promotes the degradation of cellulose and hemicellulose.					
30933826	5	16	theme	hemicellulose	758:770	arg1	degradation					729:739	the degradation	725:739	the degradation of cellulose and hemicellulose	725:770	Moreover, pyrolysis gas promotes the degradation of cellulose and hemicellulose.					
30933826	4	17	theme	pyrolysis	603:611	arg1	gas					613:615	pyrolysis gas	603:615	pyrolysis gas	603:615	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	8	18	theme	char	991:994	arg1	combustion					959:968	The combustion kinetic	955:976	The combustion kinetic of torrefied char	955:994	The combustion kinetic of torrefied char were calculated using the Friedman method and the Ozawa-Flynn-Wall method.					
30933826	2	19	theme	pyrolysis	296:304	arg1	gas					306:308	pyrolysis gas	296:308	pyrolysis gas	296:308	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	20	theme	combustion	353:362	arg1	performance					364:374	combustion performance	353:374	combustion performance	353:374	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	20	theme	combustion	353:362	arg1	properties					245:254	And the chemical and physical properties	215:254	properties	245:254	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	0	21	theme	Torrefaction	0:11	arg1	performance					13:23	Torrefaction performance	0:23	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.	0:73	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.					
30933826	3	22	from	influence	460:468	arg1	performance					551:561	the torrefaction performance	534:561	the torrefaction performance	534:561	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	4	23	theme	torrefied	676:684	arg1	char					686:689	the torrefied char	672:689	the torrefied char	672:689	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	0	24	theme	camellia	28:35	arg1	shell					37:41	camellia shell	28:41	camellia shell	28:41	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.					
30933826	8	25	theme	kinetic	970:976	arg1	combustion					959:968	The combustion kinetic	955:976	The combustion kinetic of torrefied char	955:994	The combustion kinetic of torrefied char were calculated using the Friedman method and the Ozawa-Flynn-Wall method.					
30933826	7	26	theme	pyrolysis	887:895	arg1	gas					897:899	pyrolysis gas	887:899	pyrolysis gas	887:899	And each composition in pyrolysis gas plays synergy role to the severity of torrefied char.					
30933826	4	27	dep	show	598:601	arg1	improves					617:624	improves	617:624	show pyrolysis gas improves the volatile matter content and heat value of the torrefied char	598:689	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	3	28	theme	gas	438:440	arg1	torrefaction					442:453	pyrolysis gas torrefaction	428:453	pyrolysis gas torrefaction	428:453	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	2	29	dep	pyrolysis	339:347	arg1	the					335:337	the	335:337	the	335:337	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	6	30	theme	pyrolysis	830:838	arg1	gas					840:842	pyrolysis gas and N2 atmosphere	830:860	gas	840:842	Chemical structure is different for torrefied char under pyrolysis gas and N2 atmosphere.					
30933826	7	31	from	composition	872:882	arg1	gas					897:899	pyrolysis gas	887:899	pyrolysis gas	887:899	And each composition in pyrolysis gas plays synergy role to the severity of torrefied char.					
30933826	3	32	theme	torrefaction	442:453	arg1	mechanism					415:423	the mechanism	411:423	the mechanism of pyrolysis gas torrefaction	411:453	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	8	33	theme	Ozawa-Flynn-Wall	1046:1061	arg1	method					1063:1068	the Ozawa-Flynn-Wall method	1042:1068	the Ozawa-Flynn-Wall method	1042:1068	The combustion kinetic of torrefied char were calculated using the Friedman method and the Ozawa-Flynn-Wall method.					
30933826	3	34	theme	torrefaction	538:549	arg1	performance					551:561	the torrefaction performance	534:561	the torrefaction performance	534:561	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	0	35	theme	shell	37:41	arg1	performance					13:23	Torrefaction performance	0:23	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.	0:73	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.					
30933826	3	36	theme	pyrolysis	428:436	arg1	torrefaction					442:453	pyrolysis gas torrefaction	428:453	pyrolysis gas torrefaction	428:453	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	0	37	theme	gas	59:61	arg1	atmosphere					63:72	pyrolysis gas atmosphere	49:72	pyrolysis gas atmosphere	49:72	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.					
30933826	6	38	theme	torrefied	809:817	arg1	char					819:822	torrefied char	809:822	torrefied char	809:822	Chemical structure is different for torrefied char under pyrolysis gas and N2 atmosphere.					
30933826	1	39	theme	pyrolysis	106:114	arg1	gas					116:118	the pyrolysis gas and heat from biomass routine pyrolysis	102:158	gas	116:118	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	0	40	theme	pyrolysis	49:57	arg1	atmosphere					63:72	pyrolysis gas atmosphere	49:72	pyrolysis gas atmosphere	49:72	Torrefaction performance of camellia shell under pyrolysis gas atmosphere.					
30933826	4	41	theme	heat	658:661	arg1	value					663:667	heat value	658:667	heat value	658:667	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	3	42	from	CH4	510:512	arg1	gas					527:529	pyrolysis gas	517:529	pyrolysis gas	517:529	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30933826	4	43	theme	volatile	630:637	arg1	content					646:652	volatile matter content	630:652	volatile matter content	630:652	The results show pyrolysis gas improves the volatile matter content and heat value of the torrefied char.					
30933826	2	44	theme	physical	236:243	arg1	performance					364:374	combustion performance	353:374	combustion performance	353:374	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	44	theme	physical	236:243	arg1	pyrolysis					339:347	pyrolysis	339:347	pyrolysis	339:347	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	44	theme	physical	236:243	arg1	properties					245:254	And the chemical and physical properties	215:254	properties	245:254	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	7	45	theme	torrefied	939:947	arg1	char					949:952	torrefied char	939:952	torrefied char	939:952	And each composition in pyrolysis gas plays synergy role to the severity of torrefied char.					
30933826	1	46	theme	heat	124:127	arg1	pyrolysis					150:158	the pyrolysis gas and heat from biomass routine pyrolysis	102:158	pyrolysis	150:158	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	1	47	theme	PG	200:201	arg1	atmosphere					203:212	PG atmosphere	200:212	PG atmosphere	200:212	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	7	48	theme	char	949:952	arg1	severity					927:934	the severity	923:934	the severity of torrefied char	923:952	And each composition in pyrolysis gas plays synergy role to the severity of torrefied char.					
30933826	1	49	theme	from	129:132	arg1	pyrolysis					150:158	the pyrolysis gas and heat from biomass routine pyrolysis	102:158	pyrolysis	150:158	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	5	50	theme	pyrolysis	702:710	arg1	gas					712:714	pyrolysis gas	702:714	pyrolysis gas	702:714	Moreover, pyrolysis gas promotes the degradation of cellulose and hemicellulose.					
30933826	2	51	theme	chemical	223:230	arg1	performance					364:374	combustion performance	353:374	combustion performance	353:374	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	51	theme	chemical	223:230	arg1	pyrolysis					339:347	pyrolysis	339:347	pyrolysis	339:347	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	2	51	theme	chemical	223:230	arg1	properties					245:254	And the chemical and physical properties	215:254	properties	245:254	And the chemical and physical properties of torrefied char obtained under N2 and pyrolysis gas were compared as well as the pyrolysis and combustion performance.					
30933826	1	52	theme	biomass	134:140	arg1	pyrolysis					150:158	the pyrolysis gas and heat from biomass routine pyrolysis	102:158	pyrolysis	150:158	In order to complete using the pyrolysis gas and heat from biomass routine pyrolysis, the camellia shell was torrefied under PG atmosphere.					
30933826	3	53	from	H2	498:499	arg1	gas					527:529	pyrolysis gas	517:529	pyrolysis gas	517:529	Moreover, in order to investigate the mechanism of pyrolysis gas torrefaction, the influence of each composition such as H2, CO2 and CH4 in pyrolysis gas on the torrefaction performance was also been studied.					
30711106	5	0	theme	129	753:755	arg1	%					756:756	%	756:756	%	756:756	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	0	1	from	studies	20:26	arg1	interaction					35:45	the interaction	31:45	the interaction between polyphenols and apple cell walls	31:86	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	7	2	from	reduction	997:1005	arg1	capacities					1018:1027	binding capacities	1010:1027	binding capacities of polyphenols	1010:1042	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	1	3	theme	adsorption	133:142	arg1	capacity					144:151	The adsorption capacity	129:151	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties	129:226	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	0	4	theme	cell	77:80	arg1	walls					82:86	apple cell walls	71:86	apple cell walls	71:86	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	5	5	from	pectin	717:722	arg1	walls					738:742	apple cell walls	727:742	apple cell walls	727:742	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	2	6	theme	Isothermal	246:255	arg1	adsorption					257:266	Isothermal adsorption	246:266	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations	246:332	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	7	7	theme	binding	1102:1108	arg1	sites					1110:1114	binding sites	1102:1114	binding sites	1102:1114	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	7	theme	binding	1102:1108	arg1	structural					1085:1094	structural	1085:1094	structural	1085:1094	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	3	8	theme	Langmuir	527:534	arg1	model					536:540	the Langmuir model	523:540	the Langmuir model in all cases	523:553	The isotherm data were best fitted by the Langmuir model in all cases.					
30711106	3	9	from	model	536:540	arg1	cases					549:553	all cases	545:553	all cases	545:553	The isotherm data were best fitted by the Langmuir model in all cases.					
30711106	5	10	theme	apple	727:731	arg1	walls					738:742	apple cell walls	727:742	apple cell walls	727:742	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	7	11	dep	degradation	1148:1158	arg1	i.e.					1136:1139	i.e.	1136:1139	i.e.	1136:1139	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	4	12	theme	surface	691:697	arg1	charge					699:704	surface charge	691:704	surface charge	691:704	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	0	13	theme	variety	100:106	arg1	Effects					89:95	Effects	89:95	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	5	14	theme	polyphenols	812:822	arg1	adsorption					779:788	the adsorption	775:788	the adsorption of negatively charged polyphenols	775:822	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	4	15	theme	binding	618:624	arg1	capacities					626:635	maximum binding capacities	610:635	maximum binding capacities ranging from 140 to 580 µg/mg cell walls	610:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	4	16	theme	cell	667:670	arg1	walls					672:676	580 µg/mg cell walls	657:676	580 µg/mg cell walls	657:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	7	17	theme	walls	1185:1189	arg1	sites					1110:1114	binding sites	1102:1114	binding sites	1102:1114	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	17	theme	walls	1185:1189	arg1	structural					1085:1094	structural	1085:1094	structural	1085:1094	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	18	dep	sites	1110:1114	arg1	i.e.					1097:1100	i.e.	1097:1100	i.e.	1097:1100	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	2	19	from	range	358:362	arg1	30 mM					394:398	30 mM	394:398	30 mM	394:398	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	4	20	theme	cell	594:597	arg1	walls					599:603	cell walls	594:603	cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls	594:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	1	21	theme	principal	156:164	arg1	compounds					175:183	principal phenolic compounds	156:183	principal phenolic compounds	156:183	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	0	22	theme	isotherm	11:18	arg1	studies					20:26	Adsorption isotherm studies	0:26	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	5	23	theme	%	756:756	arg1	decrease					763:770	a 129%-311% decrease	751:770	a 129%-311% decrease in the adsorption of negatively charged polyphenols	751:822	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	2	24	dep	carried	339:345	arg1	subjected					433:441	subjected	433:441	were subjected to boiling, oven-drying or freeze-drying	428:482	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	2	24	dep	carried	339:345	arg1	before					400:405	before	400:405	before	400:405	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	1	25	theme	phenolic	166:173	arg1	compounds					175:183	principal phenolic compounds	156:183	principal phenolic compounds	156:183	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	0	26	theme	Adsorption	0:9	arg1	studies					20:26	Adsorption isotherm studies	0:26	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	5	27	theme	Increased	707:715	arg1	pectin					717:722	Increased pectin	707:722	Increased pectin in apple cell walls	707:742	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	1	28	theme	compounds	175:183	arg1	capacity					144:151	The adsorption capacity	129:151	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties	129:226	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	0	29	theme	heating	109:115	arg1	Effects					89:95	Effects	89:95	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	2	30	theme	cell	417:420	arg1	walls					422:426	cell walls	417:426	cell walls	417:426	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	7	31	theme	twofold	989:995	arg1	reduction					997:1005	more than twofold reduction	979:1005	more than twofold reduction in binding capacities of polyphenols	979:1042	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	32	dep	twofold	989:995	arg1	than					984:987	than	984:987	than	984:987	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	32	dep	twofold	989:995	arg1	more					979:982	more	979:982	more	979:982	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	7	33	theme	pectin	1141:1146	arg1	degradation					1148:1158	pectin degradation	1141:1158	pectin degradation	1141:1158	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	1	34	theme	cell	190:193	arg1	walls					195:199	cell walls	190:199	cell walls from three apple varieties	190:226	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	5	35	theme	-311	757:760	arg1	%					761:761	%	761:761	%	761:761	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	3	36	theme	isotherm	489:496	arg1	data					498:501	The isotherm data	485:501	The isotherm data	485:501	The isotherm data were best fitted by the Langmuir model in all cases.					
30711106	0	37	theme	drying	121:126	arg1	Effects					89:95	Effects	89:95	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	6	38	theme	wall	951:954	arg1	interactions					956:967	polyphenol-cell wall interactions	935:967	polyphenol-cell wall interactions	935:967	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	4	39	theme	580 µg/mg	657:665	arg1	walls					672:676	580 µg/mg cell walls	657:676	580 µg/mg cell walls	657:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	5	40	theme	%	761:761	arg1	decrease					763:770	a 129%-311% decrease	751:770	a 129%-311% decrease in the adsorption of negatively charged polyphenols	751:822	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	6	41	theme	polyphenol-cell	935:949	arg1	interactions					956:967	polyphenol-cell wall interactions	935:967	polyphenol-cell wall interactions	935:967	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	5	42	theme	electrostatic	843:855	arg1	forces					867:872	electrostatic repulsive forces	843:872	electrostatic repulsive forces	843:872	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	7	43	theme	binding	1010:1016	arg1	capacities					1018:1027	binding capacities	1010:1027	binding capacities of polyphenols	1010:1042	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	6	44	contain	had	883:885	arg1	Boiling					875:881	Boiling	875:881	Boiling	875:881	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	6	44	contain	had	883:885	arg2	effect					895:900	limited effect	887:900	limited effect	887:900	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	7	45	theme	compositional	1121:1133	arg1	characteristics					1161:1175	the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics	1081:1175	characteristics	1161:1175	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	1	46	from	varieties	218:226	arg1	walls					195:199	cell walls	190:199	cell walls from three apple varieties	190:226	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	4	47	with	walls	599:603	arg1	capacities					626:635	maximum binding capacities	610:635	maximum binding capacities ranging from 140 to 580 µg/mg cell walls	610:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	6	48	theme	limited	887:893	arg1	effect					895:900	limited effect	887:900	limited effect	887:900	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	6	49	theme	cell	905:908	arg1	polysaccharides					915:929	cell wall polysaccharides	905:929	cell wall polysaccharides	905:929	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	2	50	from	30 mM	394:398	arg1	range					358:362	a range	356:362	a range of concentrations from 0.5 to 30 mM	356:398	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	2	50	from	30 mM	394:398	arg1	concentrations					367:380	concentrations	367:380	concentrations from 0.5 to 30 mM	367:398	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	2	51	dep	Freundlich	292:301	arg1	equations					324:332	equations	324:332	equations	324:332	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	6	52	theme	wall	910:913	arg1	polysaccharides					915:929	cell wall polysaccharides	905:929	cell wall polysaccharides	905:929	Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions.					
30711106	5	53	theme	repulsive	857:865	arg1	forces					867:872	electrostatic repulsive forces	843:872	electrostatic repulsive forces	843:872	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	5	54	theme	cell	733:736	arg1	walls					738:742	apple cell walls	727:742	apple cell walls	727:742	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	7	55	theme	polyphenols	1032:1042	arg1	capacities					1018:1027	binding capacities	1010:1027	binding capacities of polyphenols	1010:1042	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	0	56	dep	studies	20:26	arg1	Effects					89:95	Effects	89:95	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.	0:127	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	1	57	theme	apple	212:216	arg1	varieties					218:226	three apple varieties	206:226	three apple varieties	206:226	The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated.					
30711106	0	58	theme	apple	71:75	arg1	walls					82:86	apple cell walls	71:86	apple cell walls	71:86	Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating and drying.					
30711106	2	59	dep	30 mM	394:398	arg1	to					391:392	to	391:392	to	391:392	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	5	60	theme	charged	804:810	arg1	polyphenols					812:822	negatively charged polyphenols	793:822	negatively charged polyphenols	793:822	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
30711106	2	61	theme	concentrations	367:380	arg1	range					358:362	a range	356:362	a range of concentrations from 0.5 to 30 mM	356:398	Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying.					
30711106	4	62	theme	maximum	610:616	arg1	capacities					626:635	maximum binding capacities	610:635	maximum binding capacities ranging from 140 to 580 µg/mg cell walls	610:676	Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge.					
30711106	7	63	theme	cell	1180:1183	arg1	walls					1185:1189	cell walls	1180:1189	cell walls	1180:1189	However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.					
30711106	5	64	from	decrease	763:770	arg1	adsorption					779:788	the adsorption	775:788	the adsorption of negatively charged polyphenols	775:822	Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces.					
31335981	0	0	theme	giant	72:76	arg1	gel					111:113	the giant squid hydrolysate-polysaccharide gel	68:113	the giant squid hydrolysate-polysaccharide gel	68:113	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	3	1	theme	treatment	305:313	arg1	effect					279:284	the effect	275:284	the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	275:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	5	2	theme	rheological	635:645	arg1	measurements					647:658	The rheological measurements	631:658	The rheological measurements	631:658	The rheological measurements indicated that autoclaving led to significantly higher viscoelastic modulus and gel strength.					
31335981	4	3	theme	magnetic	477:484	arg1	resonance					486:494	nuclear magnetic resonance	469:494	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	4	4	theme	spin-spin	503:511	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	3	5	dep	microstructure	338:351	arg1	the					334:336	the	334:336	the	334:336	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	7	6	contain	had	1047:1049	arg2	effect					1090:1095	a similar, but insignificant, positive effect	1051:1095	a similar, but insignificant, positive effect	1051:1095	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	7	6	contain	had	1047:1049	arg1	Autoclaving					1035:1045	Autoclaving	1035:1045	Autoclaving	1035:1045	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	6	7	theme	polysaccharides	876:890	arg1	separation					862:871	the phase separation	852:871	the phase separation of polysaccharides and proteins/peptides	852:912	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	0	8	theme	hydrolysate-polysaccharide	84:109	arg1	gel					111:113	the giant squid hydrolysate-polysaccharide gel	68:113	the giant squid hydrolysate-polysaccharide gel	68:113	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	0	9	from	Effect	0:5	arg1	structure					55:63	structure	55:63	structure	55:63	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	0	9	from	Effect	0:5	arg1	properties					40:49	mechanical properties	29:49	mechanical properties	29:49	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	4	10	theme	stronger	582:589	arg1	ability					605:611	stronger water-binding ability	582:611	stronger water-binding ability of the GSH-P gel	582:628	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	0	11	theme	squid	78:82	arg1	gel					111:113	the giant squid hydrolysate-polysaccharide gel	68:113	the giant squid hydrolysate-polysaccharide gel	68:113	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	7	12	theme	similar	1053:1059	arg1	effect					1090:1095	a similar, but insignificant, positive effect	1051:1095	a similar, but insignificant, positive effect	1051:1095	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	6	13	theme	scattering	826:835	arg1	results					758:764	The results	754:764	The results of confocal laser scanning microscopy and small-angle X-ray scattering	754:835	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	9	14	theme	GSH-P	1305:1309	arg1	gel					1311:1313	the GSH-P gel	1301:1313	the GSH-P gel	1301:1313	This study shows that autoclaving can alter the structure and improve the properties of the GSH-P gel.					
31335981	7	15	theme	P	1120:1120	arg1	gel					1123:1125	the polysaccharide (P) gel	1100:1125	the polysaccharide (P) gel	1100:1125	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	4	16	theme	GSH-P	620:624	arg1	gel					626:628	the GSH-P gel	616:628	the GSH-P gel	616:628	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	1	17	theme	composite	173:181	arg1	foods					187:191	composite gel foods	173:191	composite gel foods	173:191	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	0	18	theme	gel	111:113	arg1	structure					55:63	structure	55:63	structure	55:63	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	0	18	theme	gel	111:113	arg1	properties					40:49	mechanical properties	29:49	mechanical properties	29:49	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	4	19	theme	nuclear	469:475	arg1	resonance					486:494	nuclear magnetic resonance	469:494	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	3	20	theme	giant	375:379	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	6	21	theme	small-angle	808:818	arg1	scattering					826:835	small-angle X-ray scattering	808:835	small-angle X-ray scattering	808:835	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	1	22	theme	gel	183:185	arg1	foods					187:191	composite gel foods	173:191	composite gel foods	173:191	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	7	23	theme	polysaccharide	1104:1117	arg1	gel					1123:1125	the polysaccharide (P) gel	1100:1125	the polysaccharide (P) gel	1100:1125	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	3	24	theme	hydrolysate-konjac	387:404	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	7	25	theme	positive	1081:1088	arg1	effect					1090:1095	a similar, but insignificant, positive effect	1051:1095	a similar, but insignificant, positive effect	1051:1095	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	6	26	theme	ordered	1006:1012	arg1	structures					1023:1032	less, but ordered, network structures	996:1032	less, but ordered, network structures	996:1032	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	6	27	theme	microscopy	793:802	arg1	results					758:764	The results	754:764	The results of confocal laser scanning microscopy and small-angle X-ray scattering	754:835	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	5	28	theme	gel	740:742	arg1	strength					744:751	gel strength	740:751	gel strength	740:751	The rheological measurements indicated that autoclaving led to significantly higher viscoelastic modulus and gel strength.					
31335981	3	29	theme	glucomannan-κ-carrageenan-locust	406:437	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	8	30	theme	polysaccharide	1188:1201	arg1	gelation					1203:1210	polysaccharide gelation	1188:1210	polysaccharide gelation	1188:1210	Thus, GSH seems to play an important role in the process of polysaccharide gelation.					
31335981	5	31	theme	higher	708:713	arg1	modulus					728:734	significantly higher viscoelastic modulus	694:734	significantly higher viscoelastic modulus	694:734	The rheological measurements indicated that autoclaving led to significantly higher viscoelastic modulus and gel strength.					
31335981	6	32	theme	scanning	784:791	arg1	microscopy					793:802	confocal laser scanning microscopy	769:802	confocal laser scanning microscopy	769:802	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	3	33	theme	GSH-P	453:457	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	0	34	theme	autoclaving	10:20	arg1	Effect					0:5	Effect	0:5	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.	0:114	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	5	35	theme	viscoelastic	715:726	arg1	modulus					728:734	significantly higher viscoelastic modulus	694:734	significantly higher viscoelastic modulus	694:734	The rheological measurements indicated that autoclaving led to significantly higher viscoelastic modulus and gel strength.					
31335981	8	36	theme	important	1155:1163	arg1	role					1165:1168	an important role	1152:1168	an important role	1152:1168	Thus, GSH seems to play an important role in the process of polysaccharide gelation.					
31335981	3	37	theme	gel	459:461	arg1	properties					357:366	properties	357:366	properties	357:366	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	3	37	theme	gel	459:461	arg1	microstructure					338:351	microstructure	338:351	microstructure	338:351	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	0	38	theme	mechanical	29:38	arg1	properties					40:49	mechanical properties	29:49	mechanical properties	29:49	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	6	39	theme	less	996:999	arg1	structures					1023:1032	less, but ordered, network structures	996:1032	less, but ordered, network structures	996:1032	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	3	40	dep	121°C	316:320	arg1	min					326:328	10 min	323:328	10 min	323:328	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	6	41	theme	proteins/peptides	896:912	arg1	separation					862:871	the phase separation	852:871	the phase separation of polysaccharides and proteins/peptides	852:912	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	3	42	theme	gel	448:450	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	3	43	theme	squid	381:385	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	4	44	theme	time	524:527	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	8	45	theme	gelation	1203:1210	arg1	process					1177:1183	the process	1173:1183	the process of polysaccharide gelation	1173:1210	Thus, GSH seems to play an important role in the process of polysaccharide gelation.					
31335981	0	46	dep	properties	40:49	arg1	the					25:27	the	25:27	the	25:27	Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.					
31335981	6	47	theme	laser	778:782	arg1	microscopy					793:802	confocal laser scanning microscopy	769:802	confocal laser scanning microscopy	769:802	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	4	48	theme	relaxation	513:522	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	7	49	theme	insignificant	1066:1078	arg1	effect					1090:1095	a similar, but insignificant, positive effect	1051:1095	a similar, but insignificant, positive effect	1051:1095	Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel.					
31335981	3	50	theme	bean	439:442	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	6	51	theme	confocal	769:776	arg1	microscopy					793:802	confocal laser scanning microscopy	769:802	confocal laser scanning microscopy	769:802	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	1	52	theme	Fish	116:119	arg1	option					152:157	an attractive option	138:157	an attractive option for preparing composite gel foods	138:191	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	1	52	theme	Fish	116:119	arg1	hydrolysates					121:132	Fish hydrolysates	116:132	Fish hydrolysates	116:132	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	4	53	theme	proton	496:501	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	4	54	theme	T2	530:531	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	3	55	theme	autoclaving	293:303	arg1	121°C					316:320	121°C	316:320	121°C	316:320	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	3	55	theme	autoclaving	293:303	arg1	treatment					305:313	the autoclaving treatment	289:313	the autoclaving treatment (121°C, 10 min)	289:329	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	9	56	theme	gel	1311:1313	arg1	properties					1287:1296	the properties	1283:1296	the properties of the GSH-P gel	1283:1313	This study shows that autoclaving can alter the structure and improve the properties of the GSH-P gel.					
31335981	3	57	from	effect	279:284	arg1	properties					357:366	properties	357:366	properties	357:366	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	3	57	from	effect	279:284	arg1	microstructure					338:351	microstructure	338:351	microstructure	338:351	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	6	58	theme	phase	856:860	arg1	separation					862:871	the phase separation	852:871	the phase separation of polysaccharides and proteins/peptides	852:912	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	6	59	theme	X-ray	820:824	arg1	scattering					826:835	small-angle X-ray scattering	808:835	small-angle X-ray scattering	808:835	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	4	60	theme	water-binding	591:603	arg1	ability					605:611	stronger water-binding ability	582:611	stronger water-binding ability of the GSH-P gel	582:628	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	4	61	theme	gel	626:628	arg1	ability					605:611	stronger water-binding ability	582:611	stronger water-binding ability of the GSH-P gel	582:628	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	1	62	theme	attractive	141:150	arg1	option					152:157	an attractive option	138:157	an attractive option for preparing composite gel foods	138:191	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	1	62	theme	attractive	141:150	arg1	hydrolysates					121:132	Fish hydrolysates	116:132	Fish hydrolysates	116:132	Fish hydrolysates are an attractive option for preparing composite gel foods.					
31335981	3	63	theme	gum	444:446	arg1	gel					459:461	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel)	371:462	Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel).					
31335981	6	64	theme	network	1015:1021	arg1	structures					1023:1032	less, but ordered, network structures	996:1032	less, but ordered, network structures	996:1032	The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures.					
31335981	4	65	theme	resonance	486:494	arg1	measurements					535:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements	465:546	The nuclear magnetic resonance proton spin-spin relaxation time (T2 ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel.					
31335981	2	66	theme	gel	237:239	arg1	properties					241:250	their gel properties	231:250	their gel properties	231:250	However, not much is known regarding their gel properties.					
30309034	8	0	theme	water-soluble	1067:1079	arg1	polysaccharide					1081:1094	the water-soluble polysaccharide	1063:1094	the water-soluble polysaccharide	1063:1094	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	6	1	theme	natural	786:792	arg1	pulp					801:804	the natural longan pulp	782:804	the natural longan pulp	782:804	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	1	2	theme	juice	177:181	arg1	potential					157:165	The prebiotic potential	143:165	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide	143:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	9	3	theme	ethanol-soluble	1177:1191	arg1	fraction					1199:1206	its ethanol-soluble sugar fraction	1173:1206	its ethanol-soluble sugar fraction	1173:1206	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	4	4	theme	total	567:571	arg1	clarity					599:605	clarity	599:605	clarity	599:605	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	4	theme	total	567:571	arg1	yield					589:593	yield	589:593	yield	589:593	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	4	theme	total	567:571	arg1	solids					581:586	total soluble solids	567:586	total soluble solids	567:586	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	9	5	theme	sugar	1193:1197	arg1	fraction					1199:1206	its ethanol-soluble sugar fraction	1173:1206	its ethanol-soluble sugar fraction	1173:1206	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	4	6	theme	juice	617:621	arg1	clarity					599:605	clarity	599:605	clarity	599:605	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	6	theme	juice	617:621	arg1	yield					589:593	yield	589:593	yield	589:593	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	6	theme	juice	617:621	arg1	solids					581:586	total soluble solids	567:586	total soluble solids	567:586	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	0	7	theme	Sucrose	105:111	arg1	Conversion					79:88	Enzymatic Conversion	69:88	Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides	69:140	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	8	8	theme	enzyme	1126:1131	arg1	treatment					1133:1141	enzyme treatment	1126:1141	enzyme treatment	1126:1141	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	2	9	theme	carbohydrate	337:348	arg1	composition					350:360	carbohydrate composition	337:360	carbohydrate composition	337:360	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	7	10	theme	enzyme	973:978	arg1	treatment					980:988	enzyme treatment	973:988	enzyme treatment	973:988	Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment.					
30309034	0	11	theme	Constituent	93:103	arg1	Sucrose					105:111	Constituent Sucrose	93:111	Constituent Sucrose	93:111	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	6	12	theme	sucrose	865:871	arg1	content					873:879	sucrose content	865:879	sucrose content	865:879	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	9	13	theme	treated	1347:1353	arg1	juice					1362:1366	the treated longan juice	1343:1366	the treated longan juice	1343:1366	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	1	14	theme	commercial	197:206	arg1	L					218:218	a commercial Viscozyme L	195:218	a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide	195:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	3	15	theme	probiotic	489:497	arg1	bacteria					499:506	probiotic bacteria	489:506	probiotic bacteria growth	489:513	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	3	16	theme	treated	465:471	arg1	juice					480:484	the treated longan juice	461:484	the treated longan juice	461:484	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	9	17	theme	longan	1355:1360	arg1	juice					1362:1366	the treated longan juice	1343:1366	the treated longan juice	1343:1366	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	2	18	theme	physicochemical	306:320	arg1	properties					322:331	The physicochemical properties	302:331	The physicochemical properties	302:331	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	3	19	theme	bacteria	499:506	arg1	growth					508:513	probiotic bacteria growth	489:513	probiotic bacteria growth	489:513	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	3	20	theme	stimulation	438:448	arg1	effects					450:456	The stimulation effects	434:456	The stimulation effects of the treated longan juice on probiotic bacteria growth	434:513	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	3	21	from	effects	450:456	arg1	growth					508:513	probiotic bacteria growth	489:513	probiotic bacteria growth	489:513	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	5	22	theme	water-soluble	683:695	arg1	content					712:718	The water-soluble polysaccharide content	679:718	The water-soluble polysaccharide content	679:718	The water-soluble polysaccharide content, including pectin, was significantly increased.					
30309034	6	23	theme	significant	841:851	arg1	decrease					853:860	a significant decrease	839:860	a significant decrease in sucrose content	839:879	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	2	24	theme	longan	369:374	arg1	juice					376:380	the longan juice	365:380	the longan juice	365:380	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	3	25	theme	juice	480:484	arg1	effects					450:456	The stimulation effects	434:456	The stimulation effects of the treated longan juice on probiotic bacteria growth	434:513	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	5	26	theme	polysaccharide	697:710	arg1	content					712:718	The water-soluble polysaccharide content	679:718	The water-soluble polysaccharide content	679:718	The water-soluble polysaccharide content, including pectin, was significantly increased.					
30309034	8	27	theme	molecular	995:1003	arg1	distribution					1012:1023	The molecular weight distribution	991:1023	The molecular weight distribution	991:1023	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	1	28	theme	Viscozyme	208:216	arg1	L					218:218	a commercial Viscozyme L	195:218	a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide	195:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	9	29	theme	thermophiles	1244:1255	arg1	growth					1221:1226	the growth	1217:1226	the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii	1217:1312	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	0	30	theme	Prebiotic	21:29	arg1	Potential					31:39	Prebiotic Potential	21:39	Prebiotic Potential	21:39	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	4	31	theme	longan	610:615	arg1	juice					617:621	longan juice	610:621	longan juice	610:621	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	9	32	theme	functional	1382:1391	arg1	foods					1393:1397	functional foods	1382:1397	functional foods	1382:1397	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	7	33	theme	Substantial	882:892	arg1	nystose					942:948	nystose	942:948	nystose	942:948	Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment.					
30309034	7	33	theme	Substantial	882:892	arg1	1-kestose					928:936	1-kestose	928:936	1-kestose	928:936	Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment.					
30309034	7	33	theme	Substantial	882:892	arg1	fructo-oligosaccharides					894:916	Substantial fructo-oligosaccharides	882:916	Substantial fructo-oligosaccharides including 1-kestose and nystose	882:948	Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment.					
30309034	9	34	theme	Streptococus	1231:1242	arg1	thermophiles					1244:1255	Streptococus thermophiles	1231:1255	Streptococus thermophiles	1231:1255	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	9	34	theme	Streptococus	1231:1242	arg1	acidophilus					1272:1282	Lactobacillus acidophilus	1258:1282	Lactobacillus acidophilus	1258:1282	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	9	34	theme	Streptococus	1231:1242	arg1	delbrueckii					1302:1312	Lactobacillus delbrueckii	1288:1312	Lactobacillus delbrueckii	1288:1312	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	0	35	theme	Longan	44:49	arg1	Juice					51:55	Longan Juice	44:55	Longan Juice	44:55	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	8	36	theme	weight	1005:1010	arg1	distribution					1012:1023	The molecular weight distribution	991:1023	The molecular weight distribution	991:1023	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	6	37	from	decrease	853:860	arg1	content					873:879	sucrose content	865:879	sucrose content	865:879	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	3	38	theme	longan	473:478	arg1	juice					480:484	the treated longan juice	461:484	the treated longan juice	461:484	The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro.					
30309034	4	39	theme	soluble	573:579	arg1	clarity					599:605	clarity	599:605	clarity	599:605	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	39	theme	soluble	573:579	arg1	yield					589:593	yield	589:593	yield	589:593	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	4	39	theme	soluble	573:579	arg1	solids					581:586	total soluble solids	567:586	total soluble solids	567:586	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
30309034	6	40	theme	treated	818:824	arg1	juice					826:830	the enzyme treated juice	807:830	the enzyme treated juice	807:830	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	0	41	theme	Juice	51:55	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	0	41	theme	Juice	51:55	arg1	Potential					31:39	Prebiotic Potential	21:39	Prebiotic Potential	21:39	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	1	42	theme	longan	170:175	arg1	juice					177:181	longan juice	170:181	longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide	170:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	6	43	theme	enzyme	811:816	arg1	juice					826:830	the enzyme treated juice	807:830	the enzyme treated juice	807:830	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	9	44	theme	treated	1148:1154	arg1	juice					1163:1167	The treated longan juice	1144:1167	The treated longan juice	1144:1167	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	8	45	theme	monosaccharide	1033:1046	arg1	composition					1048:1058	the monosaccharide composition	1029:1058	the monosaccharide composition of the water-soluble polysaccharide	1029:1094	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	1	46	theme	prebiotic	147:155	arg1	potential					157:165	The prebiotic potential	143:165	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide	143:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	8	47	theme	polysaccharide	1081:1094	arg1	composition					1048:1058	the monosaccharide composition	1029:1058	the monosaccharide composition of the water-soluble polysaccharide	1029:1094	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	8	47	theme	polysaccharide	1081:1094	arg1	distribution					1012:1023	The molecular weight distribution	991:1023	The molecular weight distribution	991:1023	The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment.					
30309034	1	48	theme	constituent	238:248	arg1	sucrose					250:256	constituent sucrose	238:256	constituent sucrose	238:256	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	2	49	theme	juice	376:380	arg1	properties					322:331	The physicochemical properties	302:331	The physicochemical properties	302:331	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	2	49	theme	juice	376:380	arg1	composition					350:360	carbohydrate composition	337:360	carbohydrate composition	337:360	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	1	50	theme	sucrose	250:256	arg1	conversion					224:233	conversion	224:233	conversion of constituent sucrose to fructo-oligosaccharide	224:282	The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated.					
30309034	0	51	theme	Enzymatic	69:77	arg1	Conversion					79:88	Enzymatic Conversion	69:88	Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides	69:140	Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides.					
30309034	9	52	theme	good	1325:1328	arg1	potential					1330:1338	a good potential	1323:1338	a good potential of the treated longan juice for producing functional foods and nutraceuticals	1323:1416	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	9	53	theme	longan	1156:1161	arg1	juice					1163:1167	The treated longan juice	1144:1167	The treated longan juice	1144:1167	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	9	54	theme	juice	1362:1366	arg1	potential					1330:1338	a good potential	1323:1338	a good potential of the treated longan juice for producing functional foods and nutraceuticals	1323:1416	The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.					
30309034	6	55	theme	longan	794:799	arg1	pulp					801:804	the natural longan pulp	782:804	the natural longan pulp	782:804	Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content.					
30309034	2	56	theme	enzymatic	413:421	arg1	treatment					423:431	enzymatic treatment	413:431	enzymatic treatment	413:431	The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment.					
30309034	4	57	theme	enzyme	661:666	arg1	treatment					668:676	enzyme treatment	661:676	enzyme treatment	661:676	The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment.					
31421168	9	0	from	potential	1227:1235	arg1	feed					1262:1265	feed	1262:1265	feed	1262:1265	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	9	0	from	potential	1227:1235	arg1	fields					1286:1291	pharmaceutical fields	1271:1291	pharmaceutical fields	1271:1291	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	9	0	from	potential	1227:1235	arg1	food					1256:1259	food	1256:1259	food	1256:1259	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	7	1	theme	magnetic	887:894	arg1	resonance					896:904	13C nuclear magnetic resonance	875:904	13C nuclear magnetic resonance (NMR)	875:910	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	7	1	theme	magnetic	887:894	arg1	NMR					907:909	NMR	907:909	NMR	907:909	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	1	2	theme	homemade	155:162	arg1	product					177:183	the homemade fermentation product	151:183	the homemade fermentation product of hami melon	151:197	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	8	3	theme	water	997:1001	arg1	WSI					1021:1023	WSI	1021:1023	WSI	1021:1023	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	3	theme	water	997:1001	arg1	index					1014:1018	a high water solubility index	990:1018	a high water solubility index (WSI)	990:1024	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	1	4	attach	isolated	137:144	arg1	product					177:183	the homemade fermentation product	151:183	the homemade fermentation product of hami melon	151:197	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	1	4	attach	isolated	137:144	arg2	EPS					108:110	EPS	108:110	EPS	108:110	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	1	4	attach	isolated	137:144	arg2	exopolysaccharide					89:105	An exopolysaccharide	86:105	An exopolysaccharide (EPS)-producing bacterium	86:131	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	8	5	dep	capacity	1044:1051	arg1	%					1072:1072	91.90 ± 2.45%	1060:1072	91.90 ± 2.45%	1060:1072	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	5	dep	capacity	1044:1051	arg1	%					1092:1092	509.45 ± 28.59%	1078:1092	509.45 ± 28.59%	1078:1092	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	4	6	theme	lactis	378:383	arg1	EPS					388:390	lactis L2 EPS	378:390	lactis L2 EPS	378:390	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	1	7	theme	fermentation	164:175	arg1	product					177:183	the homemade fermentation product	151:183	the homemade fermentation product of hami melon	151:197	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	7	8	theme	D-pyranose	801:810	arg1	residues					812:819	α-(1 → 6)-linked D-pyranose residues	784:819	α-(1 → 6)-linked D-pyranose residues	784:819	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	9	theme	holding	1036:1042	arg1	WHC					1054:1056	WHC	1054:1056	WHC	1054:1056	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	9	theme	holding	1036:1042	arg1	capacity					1044:1051	water holding capacity	1030:1051	water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively)	1030:1107	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	4	10	theme	molecular	457:465	arg1	weight					467:472	its molecular weight	453:472	its molecular weight	453:472	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	2	11	theme	CHL	329:331	arg1	test					333:336	the API 50 CHL test	318:336	the API 50 CHL test	318:336	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	5	12	theme	microscopic	559:569	arg1	compact					592:598	compact	592:598	compact	592:598	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	5	12	theme	microscopic	559:569	arg1	characteristics					571:585	The EPS microscopic characteristics	551:585	The EPS microscopic characteristics	551:585	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	8	13	theme	solubility	1003:1012	arg1	WSI					1021:1023	WSI	1021:1023	WSI	1021:1023	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	13	theme	solubility	1003:1012	arg1	index					1014:1018	a high water solubility index	990:1018	a high water solubility index (WSI)	990:1024	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	14	theme	water	1030:1034	arg1	WHC					1054:1056	WHC	1054:1056	WHC	1054:1056	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	14	theme	water	1030:1034	arg1	capacity					1044:1051	water holding capacity	1030:1051	water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively)	1030:1107	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	5	15	theme	electron	637:644	arg1	SEM					658:660	SEM	658:660	SEM	658:660	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	5	15	theme	electron	637:644	arg1	microscopy					646:655	scanning electron microscopy	628:655	scanning electron microscopy (SEM)	628:661	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	7	16	dep	Fourier	835:841	arg1	transform					843:851	transform	843:851	transform infrared (FT-IR)	843:868	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	1	17	dep	isolated	137:144	arg1	var					213:215	var	213:215	var	213:215	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	9	18	theme	pharmaceutical	1271:1284	arg1	fields					1286:1291	pharmaceutical fields	1271:1291	pharmaceutical fields	1271:1291	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	4	19	theme	L2	385:386	arg1	EPS					388:390	lactis L2 EPS	378:390	lactis L2 EPS	378:390	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	7	20	dep	transform	843:851	arg1	infrared					853:860	infrared	853:860	transform infrared (FT-IR)	843:868	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	6	21	theme	force	671:675	arg1	AFM					689:691	AFM	689:691	AFM	689:691	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	6	21	theme	force	671:675	arg1	microscopy					677:686	Atomic force microscopy	664:686	Atomic force microscopy (AFM)	664:692	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	6	22	theme	Atomic	664:669	arg1	AFM					689:691	AFM	689:691	AFM	689:691	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	6	22	theme	Atomic	664:669	arg1	microscopy					677:686	Atomic force microscopy	664:686	Atomic force microscopy (AFM)	664:692	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	7	23	link	-linked	793:799	arg1	residues					812:819	α-(1 → 6)-linked D-pyranose residues	784:819	α-(1 → 6)-linked D-pyranose residues	784:819	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	24	with	capacity	1044:1051	arg1	solvents					1167:1174	many organic solvents	1154:1174	many organic solvents	1154:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	1	25	theme	hami	188:191	arg1	melon					193:197	hami melon	188:197	hami melon	188:197	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	3	26	theme	Leu	373:375	arg1	composition					358:368	The monosaccharide composition	339:368	The monosaccharide composition of Leu	339:375	The monosaccharide composition of Leu.					
31421168	4	27	theme	exclusion	517:525	arg1	HPSEC					543:547	HPSEC	543:547	HPSEC	543:547	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	4	27	theme	exclusion	517:525	arg1	chromatography					527:540	high-performance size exclusion chromatography	495:540	high-performance size exclusion chromatography (HPSEC)	495:548	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	2	28	theme	sequence	296:303	arg1	analysis					305:312	16S rDNA sequence analysis	287:312	16S rDNA sequence analysis	287:312	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	1	29	theme	melon	193:197	arg1	product					177:183	the homemade fermentation product	151:183	the homemade fermentation product of hami melon	151:197	An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var.					
31421168	3	30	theme	monosaccharide	343:356	arg1	composition					358:368	The monosaccharide composition	339:368	The monosaccharide composition of Leu	339:375	The monosaccharide composition of Leu.					
31421168	4	31	theme	size	512:515	arg1	HPSEC					543:547	HPSEC	543:547	HPSEC	543:547	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	4	31	theme	size	512:515	arg1	chromatography					527:540	high-performance size exclusion chromatography	495:540	high-performance size exclusion chromatography (HPSEC)	495:548	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	6	32	theme	polymeric	725:733	arg1	matrix					735:740	a smooth and consistent polymeric matrix	701:740	a smooth and consistent polymeric matrix	701:740	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	2	33	theme	rDNA	291:294	arg1	analysis					305:312	16S rDNA sequence analysis	287:312	16S rDNA sequence analysis	287:312	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	5	34	theme	EPS	555:557	arg1	compact					592:598	compact	592:598	compact	592:598	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	5	34	theme	EPS	555:557	arg1	characteristics					571:585	The EPS microscopic characteristics	551:585	The EPS microscopic characteristics	551:585	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	2	35	theme	16S	287:289	arg1	analysis					305:312	16S rDNA sequence analysis	287:312	16S rDNA sequence analysis	287:312	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	0	36	theme	exopolysaccharide	40:56	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	0	36	theme	exopolysaccharide	40:56	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	7	37	theme	-linked	793:799	arg1	residues					812:819	α-(1 → 6)-linked D-pyranose residues	784:819	α-(1 → 6)-linked D-pyranose residues	784:819	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	7	38	theme	HSQC	956:959	arg1	spectra					962:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	39	theme	good	1121:1124	arg1	properties					1138:1147	good emulsifying properties	1121:1147	good emulsifying properties with many organic solvents	1121:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	9	40	theme	applicable	1216:1225	arg1	potential					1227:1235	the applicable potential	1212:1235	the applicable potential of this EPS in the food, feed and pharmaceutical fields	1212:1291	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	0	41	from	L2	82:83	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	0	41	from	L2	82:83	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	6	42	theme	consistent	714:723	arg1	matrix					735:740	a smooth and consistent polymeric matrix	701:740	a smooth and consistent polymeric matrix	701:740	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	0	43	theme	Leuconostoc	63:73	arg1	L2					82:83	Leuconostoc lactis L2	63:83	Leuconostoc lactis L2	63:83	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	7	44	theme	coherence	945:953	arg1	spectra					962:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	4	45	theme	gas	424:426	arg1	GC					444:445	GC	444:445	GC	444:445	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	4	45	theme	gas	424:426	arg1	chromatography					428:441	gas chromatography	424:441	gas chromatography (GC)	424:446	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	7	46	theme	heteronuclear	916:928	arg1	spectra					962:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	4	47	theme	high-performance	495:510	arg1	HPSEC					543:547	HPSEC	543:547	HPSEC	543:547	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	4	47	theme	high-performance	495:510	arg1	chromatography					527:540	high-performance size exclusion chromatography	495:540	high-performance size exclusion chromatography (HPSEC)	495:548	lactis L2 EPS was determined to be glucose by gas chromatography (GC), and its molecular weight was 3.7 × 103 kDa by high-performance size exclusion chromatography (HPSEC).					
31421168	8	48	theme	high	992:995	arg1	WSI					1021:1023	WSI	1021:1023	WSI	1021:1023	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	48	theme	high	992:995	arg1	index					1014:1018	a high water solubility index	990:1018	a high water solubility index (WSI)	990:1024	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	5	49	theme	scanning	628:635	arg1	SEM					658:660	SEM	658:660	SEM	658:660	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	5	49	theme	scanning	628:635	arg1	microscopy					646:655	scanning electron microscopy	628:655	scanning electron microscopy (SEM)	628:661	The EPS microscopic characteristics were compact, branched and tubular under scanning electron microscopy (SEM).					
31421168	6	50	theme	smooth	703:708	arg1	matrix					735:740	a smooth and consistent polymeric matrix	701:740	a smooth and consistent polymeric matrix	701:740	Atomic force microscopy (AFM) showed a smooth and consistent polymeric matrix.					
31421168	2	51	theme	API	322:324	arg1	test					333:336	the API 50 CHL test	318:336	the API 50 CHL test	318:336	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	7	52	theme	single	930:935	arg1	spectra					962:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	2	53	theme	lactis	261:266	arg1	L2					268:269	Leuconostoc lactis L2	249:269	Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test	249:336	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	7	54	theme	13C	875:877	arg1	resonance					896:904	13C nuclear magnetic resonance	875:904	13C nuclear magnetic resonance (NMR)	875:910	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	7	54	theme	13C	875:877	arg1	NMR					907:909	NMR	907:909	NMR	907:909	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	55	theme	many	1154:1157	arg1	solvents					1167:1174	many organic solvents	1154:1174	many organic solvents	1154:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	9	56	theme	EPS	1245:1247	arg1	potential					1227:1235	the applicable potential	1212:1235	the applicable potential of this EPS in the food, feed and pharmaceutical fields	1212:1291	These results collectively suggest the applicable potential of this EPS in the food, feed and pharmaceutical fields.					
31421168	7	57	theme	quantum	937:943	arg1	spectra					962:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	heteronuclear single quantum coherence (HSQC) spectra	916:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	2	58	theme	Leuconostoc	249:259	arg1	L2					268:269	Leuconostoc lactis L2	249:269	Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test	249:336	saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test.					
31421168	8	59	theme	emulsifying	1126:1136	arg1	properties					1138:1147	good emulsifying properties	1121:1147	good emulsifying properties with many organic solvents	1121:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	8	60	with	properties	1138:1147	arg1	solvents					1167:1174	many organic solvents	1154:1174	many organic solvents	1154:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	7	61	theme	linear	757:762	arg1	polymer					764:770	a linear polymer	755:770	a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra	755:968	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	7	61	theme	linear	757:762	arg1	EPS					747:749	The EPS	743:749	The EPS	743:749	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	62	with	index	1014:1018	arg1	solvents					1167:1174	many organic solvents	1154:1174	many organic solvents	1154:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	0	63	theme	lactis	75:80	arg1	L2					82:83	Leuconostoc lactis L2	63:83	Leuconostoc lactis L2	63:83	Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.					
31421168	7	64	theme	nuclear	879:885	arg1	resonance					896:904	13C nuclear magnetic resonance	875:904	13C nuclear magnetic resonance (NMR)	875:910	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	7	64	theme	nuclear	879:885	arg1	NMR					907:909	NMR	907:909	NMR	907:909	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
31421168	8	65	theme	organic	1159:1165	arg1	solvents					1167:1174	many organic solvents	1154:1174	many organic solvents	1154:1174	This EPS exhibited a high water solubility index (WSI) and water holding capacity (WHC) (91.90 ± 2.45% and 509.45 ± 28.59%, respectively), as well as good emulsifying properties with many organic solvents.					
31421168	7	66	dep	infrared	853:860	arg1	FT-IR					863:867	FT-IR	863:867	FT-IR	863:867	The EPS was a linear polymer composed of α-(1 → 6)-linked D-pyranose residues, based on the Fourier transform infrared (FT-IR), 1H, 13C nuclear magnetic resonance (NMR) and heteronuclear single quantum coherence (HSQC) spectra.					
29534472	11	0	theme	I.	1409:1410	arg1	parts					1432:1436	I. glandulifera aerial parts	1409:1436	I. glandulifera aerial parts	1409:1436	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	4	1	theme	molecular	513:521	arg1	weight					523:528	a low molecular weight	507:528	a low molecular weight part that consisted of components of similar molecular weight	507:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	5	2	theme	aerial	657:662	arg1	parts					664:668	aerial parts	657:668	aerial parts	657:668	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	3	dep	MDa	741:743	arg1	up					731:732	up	731:732	up	731:732	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	4	theme	components	720:729	arg1	components					720:729	high molecular weight components	698:729	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	4	theme	components	720:729	arg1	amounts					687:693	considerable amounts	674:693	considerable amounts of high molecular weight components up to 2.3 MDa	674:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	8	5	theme	obtained	1030:1037	arg1	polysaccharides					1039:1053	obtained polysaccharides	1030:1053	obtained polysaccharides	1030:1053	Moreover, in vitro bioactivity of obtained polysaccharides were evaluated.					
29534472	10	6	dep	balsamina	1303:1311	arg1	roots					1344:1348	parviflora roots	1333:1348	parviflora roots	1333:1348	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	10	6	dep	balsamina	1303:1311	arg1	parts					1320:1324	aerial parts	1313:1324	aerial parts	1313:1324	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	9	7	theme	scavenging	1231:1240	arg1	assays					1242:1247	radical scavenging assays	1223:1247	radical scavenging assays	1223:1247	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	9	8	theme	antioxidant	1075:1085	arg1	activity					1087:1094	The antioxidant activity	1071:1094	The antioxidant activity	1071:1094	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	8	9	theme	polysaccharides	1039:1053	arg1	bioactivity					1015:1025	in vitro bioactivity	1006:1025	in vitro bioactivity of obtained polysaccharides	1006:1053	Moreover, in vitro bioactivity of obtained polysaccharides were evaluated.					
29534472	8	10	theme	in	1006:1007	arg1	bioactivity					1015:1025	in vitro bioactivity	1006:1025	in vitro bioactivity of obtained polysaccharides	1006:1053	Moreover, in vitro bioactivity of obtained polysaccharides were evaluated.					
29534472	7	11	theme	galacturonic	977:988	arg1	acid					990:993	galacturonic acid	977:993	galacturonic acid	977:993	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	3	12	theme	aerial	411:416	arg1	parts					418:422	I. balsamina aerial parts	398:422	I. balsamina aerial parts	398:422	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	5	13	theme	considerable	674:685	arg1	components					720:729	high molecular weight components	698:729	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	13	theme	considerable	674:685	arg1	amounts					687:693	considerable amounts	674:693	considerable amounts of high molecular weight components up to 2.3 MDa	674:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	1	14	from	Impatiens	187:195	arg1	bioactivity					132:142	bioactivity	132:142	bioactivity	132:142	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	1	14	from	Impatiens	187:195	arg1	polysaccharides					161:175	water-soluble polysaccharides	147:175	water-soluble polysaccharides from four Impatiens	147:195	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	1	14	from	Impatiens	187:195	arg1	characterization					111:126	Preliminary characterization	99:126	Preliminary characterization	99:126	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	11	15	theme	IL-8	1492:1495	arg1	production					1478:1487	the production	1474:1487	the production of IL-8 by 32.7 ± 10.5%	1474:1511	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	10	16	theme	aerial	1313:1318	arg1	parts					1320:1324	aerial parts	1313:1324	aerial parts	1313:1324	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	4	17	theme	SEC	425:427	arg1	chromatograms					461:473	SEC (Size exclusion chromatography) chromatograms	425:473	SEC (Size exclusion chromatography) chromatograms	425:473	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	7	18	theme	polysaccharide	906:919	arg1	fractions					921:929	All polysaccharide fractions	902:929	All polysaccharide fractions	902:929	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	7	19	contain	contain	969:975	arg1	fractions					921:929	All polysaccharide fractions	902:929	All polysaccharide fractions	902:929	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	7	19	contain	contain	969:975	arg2	acid					990:993	galacturonic acid	977:993	galacturonic acid	977:993	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	4	20	theme	low	509:511	arg1	weight					523:528	a low molecular weight	507:528	a low molecular weight part that consisted of components of similar molecular weight	507:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	7	21	theme	parviflora	946:955	arg1	roots					957:961	I. parviflora roots	943:961	I. parviflora roots	943:961	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	1	22	theme	Preliminary	99:109	arg1	characterization					111:126	Preliminary characterization	99:126	Preliminary characterization	99:126	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	3	23	theme	balsamina	401:409	arg1	parts					418:422	I. balsamina aerial parts	398:422	I. balsamina aerial parts	398:422	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	11	24	theme	glandulifera	1412:1423	arg1	parts					1432:1436	I. glandulifera aerial parts	1409:1436	I. glandulifera aerial parts	1409:1436	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	5	25	theme	aerial	597:602	arg1	parts					604:608	parts	604:608	parts	604:608	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	8	26	dep	in	1006:1007	arg1	vitro					1009:1013	vitro	1009:1013	vitro	1009:1013	Moreover, in vitro bioactivity of obtained polysaccharides were evaluated.					
29534472	0	27	theme	Preliminary	0:10	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	4	28	theme	molecular	575:583	arg1	weight					585:590	similar molecular weight	567:590	similar molecular weight	567:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	5	29	theme	glandulifera	644:655	arg1	parts					604:608	parts	604:608	parts	604:608	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	29	theme	glandulifera	644:655	arg1	roots					614:618	roots	614:618	roots	614:618	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	6	30	theme	Impatiens	791:799	arg1	polysaccharides					801:815	Impatiens polysaccharides	791:815	Impatiens polysaccharides	791:815	The sugar composition analysis revealed that Impatiens polysaccharides consisted primarily of galactose, arabinose, rhamnose, mannose, xylose, and glucose.					
29534472	3	31	from	%	358:358	arg1	range					336:340	range	336:340	range	336:340	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	4	32	theme	similar	567:573	arg1	weight					585:590	similar molecular weight	567:590	similar molecular weight	567:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	5	33	theme	weight	713:718	arg1	components					720:729	high molecular weight components	698:729	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	34	theme	balsamina	626:634	arg1	parts					604:608	parts	604:608	parts	604:608	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	34	theme	balsamina	626:634	arg1	roots					614:618	roots	614:618	roots	614:618	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	0	35	dep	Polysaccharides	82:96	arg1	Bioactivities					33:45	Bioactivities	33:45	Bioactivities	33:45	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	0	35	dep	Polysaccharides	82:96	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	11	36	theme	±	1505:1505	arg1	%					1511:1511	32.7 ± 10.5%	1500:1511	32.7 ± 10.5%	1500:1511	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	3	37	theme	I.	398:399	arg1	parts					418:422	I. balsamina aerial parts	398:422	I. balsamina aerial parts	398:422	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	11	38	theme	aerial	1425:1430	arg1	parts					1432:1436	I. glandulifera aerial parts	1409:1436	I. glandulifera aerial parts	1409:1436	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	3	39	theme	polysaccharides	320:334	arg1	yields					310:315	The yields	306:315	The yields of polysaccharides	306:334	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	9	40	theme	2,2-diphenyl-1-picrylhydrazyl	1126:1154	arg1	basis					1117:1121	the basis	1113:1121	the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays	1113:1247	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	4	41	theme	weight	585:590	arg1	components					553:562	components	553:562	components of similar molecular weight	553:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	10	42	theme	antioxidant	1262:1272	arg1	activity					1274:1281	The highest antioxidant activity	1250:1281	The highest antioxidant activity	1250:1281	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	10	43	theme	parviflora	1333:1342	arg1	roots					1344:1348	parviflora roots	1333:1348	parviflora roots	1333:1348	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	9	44	theme	2,2-azino-bis-	1167:1180	arg1	basis					1117:1121	the basis	1113:1121	the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays	1113:1247	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	12	45	theme	antioxidants	1594:1605	arg1	species					1549:1555	Impatiens species	1539:1555	Impatiens species	1539:1555	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
29534472	12	45	theme	antioxidants	1594:1605	arg1	source					1584:1589	a new source	1578:1589	a new source of antioxidants	1578:1605	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
29534472	12	46	theme	Impatiens	1539:1547	arg1	species					1549:1555	Impatiens species	1539:1555	Impatiens species	1539:1555	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
29534472	12	46	theme	Impatiens	1539:1547	arg1	source					1584:1589	a new source	1578:1589	a new source of antioxidants	1578:1605	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
29534472	9	47	theme	radical	1223:1229	arg1	assays					1242:1247	radical scavenging assays	1223:1247	radical scavenging assays	1223:1247	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	0	48	theme	L.	65:66	arg1	Polysaccharides					82:96	L. Water-Soluble Polysaccharides	65:96	L. Water-Soluble Polysaccharides	65:96	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	0	49	theme	Water-Soluble	68:80	arg1	Polysaccharides					82:96	L. Water-Soluble Polysaccharides	65:96	L. Water-Soluble Polysaccharides	65:96	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	1	50	theme	water-soluble	147:159	arg1	polysaccharides					161:175	water-soluble polysaccharides	147:175	water-soluble polysaccharides from four Impatiens	147:195	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	0	51	theme	Impatiens	55:63	arg1	Bioactivities					33:45	Bioactivities	33:45	Bioactivities	33:45	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	0	51	theme	Impatiens	55:63	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization and Bioactivities of Some Impatiens L. Water-Soluble Polysaccharides.					
29534472	4	52	dep	weight	523:528	arg1	part					530:533	part	530:533	a low molecular weight part that consisted of components of similar molecular weight	507:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	4	53	theme	exclusion	435:443	arg1	chromatography					445:458	Size exclusion chromatography	430:458	Size exclusion chromatography	430:458	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	4	53	theme	exclusion	435:443	arg1	SEC					425:427	SEC	425:427	SEC (Size exclusion chromatography) chromatograms	425:473	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	6	54	theme	composition	756:766	arg1	analysis					768:775	The sugar composition analysis	746:775	The sugar composition analysis	746:775	The sugar composition analysis revealed that Impatiens polysaccharides consisted primarily of galactose, arabinose, rhamnose, mannose, xylose, and glucose.					
29534472	1	55	theme	polysaccharides	161:175	arg1	bioactivity					132:142	bioactivity	132:142	bioactivity	132:142	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	1	55	theme	polysaccharides	161:175	arg1	characterization					111:126	Preliminary characterization	99:126	Preliminary characterization	99:126	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	1	56	from	bioactivity	132:142	arg1	Impatiens					187:195	four Impatiens	182:195	four Impatiens	182:195	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	7	57	theme	I.	943:944	arg1	roots					957:961	I. parviflora roots	943:961	I. parviflora roots	943:961	All polysaccharide fractions, except for I. parviflora roots, also contain galacturonic acid.					
29534472	5	58	theme	high	698:701	arg1	weight					713:718	high molecular weight	698:718	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	4	59	theme	Size	430:433	arg1	chromatography					445:458	Size exclusion chromatography	430:458	Size exclusion chromatography	430:458	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	4	59	theme	Size	430:433	arg1	SEC					425:427	SEC	425:427	SEC (Size exclusion chromatography) chromatograms	425:473	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	6	60	theme	sugar	750:754	arg1	analysis					768:775	The sugar composition analysis	746:775	The sugar composition analysis	746:775	The sugar composition analysis revealed that Impatiens polysaccharides consisted primarily of galactose, arabinose, rhamnose, mannose, xylose, and glucose.					
29534472	5	61	dep	balsamina	626:634	arg1	parts					664:668	aerial parts	657:668	aerial parts	657:668	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	62	theme	molecular	703:711	arg1	weight					713:718	high molecular weight	698:718	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	9	63	dep	2,2-diphenyl-1-picrylhydrazyl	1126:1154	arg1	assays					1242:1247	radical scavenging assays	1223:1247	radical scavenging assays	1223:1247	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	4	64	contain	contained	497:505	arg1	samples					489:495	all samples	485:495	all samples	485:495	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	4	64	contain	contained	497:505	arg2	weight					523:528	a low molecular weight	507:528	a low molecular weight part that consisted of components of similar molecular weight	507:590	SEC (Size exclusion chromatography) chromatograms show that all samples contained a low molecular weight part that consisted of components of similar molecular weight.					
29534472	1	65	from	characterization	111:126	arg1	Impatiens					187:195	four Impatiens	182:195	four Impatiens	182:195	Preliminary characterization and bioactivity of water-soluble polysaccharides from four Impatiens species-I.					
29534472	5	66	contain	had	670:672	arg1	parts					604:608	parts	604:608	parts	604:608	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	66	contain	had	670:672	arg2	amounts					687:693	considerable amounts	674:693	considerable amounts of high molecular weight components up to 2.3 MDa	674:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	66	contain	had	670:672	arg2	components					720:729	high molecular weight components	698:729	high molecular weight components up to 2.3 MDa	698:743	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	5	66	contain	had	670:672	arg1	roots					614:618	roots	614:618	roots	614:618	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	3	67	theme	I.	364:365	arg1	roots					378:382	I. parviflora roots	364:382	I. parviflora roots	364:382	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	11	68	from	parts	1432:1436	arg1	able					1443:1446	able	1443:1446	able	1443:1446	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	11	68	from	parts	1432:1436	arg1	polysaccharides					1388:1402	only the polysaccharides	1379:1402	only the polysaccharides from I. glandulifera aerial parts	1379:1436	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	9	69	theme	3-ethyl-benzthia-6-sulfonic	1182:1208	arg1	2,2-azino-bis-					1167:1180	2,2-azino-bis-	1167:1180	2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS)	1167:1221	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	9	69	theme	3-ethyl-benzthia-6-sulfonic	1182:1208	arg1	acid					1210:1213	3-ethyl-benzthia-6-sulfonic acid	1182:1213	3-ethyl-benzthia-6-sulfonic acid	1182:1213	The antioxidant activity was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethyl-benzthia-6-sulfonic acid) (ABTS) radical scavenging assays.					
29534472	10	70	theme	highest	1254:1260	arg1	activity					1274:1281	The highest antioxidant activity	1250:1281	The highest antioxidant activity	1250:1281	The highest antioxidant activity was obtained for I. balsamina aerial parts and I. parviflora roots.					
29534472	3	71	theme	parviflora	367:376	arg1	roots					378:382	I. parviflora roots	364:382	I. parviflora roots	364:382	The yields of polysaccharides range widely from 1.97% for I. parviflora roots to 18.63% for I. balsamina aerial parts.					
29534472	11	72	theme	tested	1361:1366	arg1	fractions					1368:1376	the tested fractions	1357:1376	the tested fractions	1357:1376	Among the tested fractions, only the polysaccharides from I. glandulifera aerial parts were able to significantly decrease the production of IL-8 by 32.7 ± 10.5%.					
29534472	5	73	dep	parts	604:608	arg1	The					593:595	The	593:595	The	593:595	The aerial parts and roots of I. balsamina, and I. glandulifera aerial parts had considerable amounts of high molecular weight components up to 2.3 MDa.					
29534472	12	74	theme	new	1580:1582	arg1	species					1549:1555	Impatiens species	1539:1555	Impatiens species	1539:1555	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
29534472	12	74	theme	new	1580:1582	arg1	source					1584:1589	a new source	1578:1589	a new source of antioxidants	1578:1605	The results suggest that Impatiens species can be considered as a new source of antioxidants.					
31308400	8	0	theme	anti-AF-AGE	1116:1126	arg1	antibody					1128:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	1	1	theme	alternate	173:181	arg1	pathway					159:165	The anhydrofructose pathway	139:165	The anhydrofructose pathway	139:165	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	1	1	theme	alternate	173:181	arg1	pathway					183:189	an alternate pathway	170:189	an alternate pathway for glycogen degradation by α-1,4-glucan lyase	170:236	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	7	2	theme	carboxyethyl	1068:1079	arg1	lysine-BSA					1081:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	5	3	theme	1,5-AF	678:683	arg1	role					670:673	the role	666:673	the role of 1,5-AF in protein modification	666:707	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	2	4	from	role	353:356	arg1	mammals					364:370	mammals	364:370	mammals	364:370	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	7	5	theme	/N-	1064:1066	arg1	lysine-BSA					1081:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	12	6	theme	first	1511:1515	arg1	This					1499:1502	This	1499:1502	This	1499:1502	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	12	6	theme	first	1511:1515	arg1	report					1517:1522	the first report	1507:1522	the first report about in vivo detection of AF-AGEs with a novel structural epitope	1507:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	12	7	dep	in	1530:1531	arg1	vivo					1533:1536	vivo	1533:1536	vivo	1533:1536	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	3	8	theme	advanced	412:419	arg1	end-products					431:442	advanced glycation end-products	412:442	advanced glycation end-products (AGEs)	412:449	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	3	8	theme	advanced	412:419	arg1	AGEs					445:448	AGEs	445:448	AGEs	445:448	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	8	9	link	enzyme-linked	1192:1204	arg1	assay					1220:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	12	10	with	detection	1538:1546	arg1	epitope					1583:1589	a novel structural epitope	1564:1589	a novel structural epitope	1564:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	5	11	theme	protein	688:694	arg1	modification					696:707	protein modification	688:707	protein modification	688:707	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	7	12	theme	anti-AF-AGE	958:968	arg1	antiserum					970:978	anti-AF-AGE antiserum	958:978	anti-AF-AGE antiserum	958:978	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	3	13	theme	diabetes	504:511	arg1	mellitus					513:520	diabetes mellitus	504:520	diabetes mellitus	504:520	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	8	14	theme	competitive	1180:1190	arg1	assay					1220:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	6	15	theme	serum	825:829	arg1	albumin					831:837	rabbit serum albumin	818:837	rabbit serum albumin (RSA)	818:843	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	6	15	theme	serum	825:829	arg1	RSA					840:842	RSA	840:842	RSA	840:842	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	11	16	theme	cultured	1463:1470	arg1	hepatocytes					1472:1482	cultured hepatocytes	1463:1482	cultured hepatocytes (HepG2 cells)	1463:1496	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	11	16	theme	cultured	1463:1470	arg1	cells					1491:1495	HepG2 cells	1485:1495	HepG2 cells	1485:1495	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	0	17	theme	glycogen	118:125	arg1	1,5-anhydro-D-fructose					92:113	1,5-anhydro-D-fructose	92:113	1,5-anhydro-D-fructose	92:113	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	0	17	theme	glycogen	118:125	arg1	metabolite					127:136	a glycogen metabolite	116:136	a glycogen metabolite	116:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	6	18	theme	rabbit	818:823	arg1	albumin					831:837	rabbit serum albumin	818:837	rabbit serum albumin (RSA)	818:843	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	6	18	theme	rabbit	818:823	arg1	RSA					840:842	RSA	840:842	RSA	840:842	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	2	19	theme	central	293:299	arg1	intermediate					301:312	the central intermediate	289:312	the central intermediate of this pathway	289:328	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	2	19	theme	central	293:299	arg1	1,5-anhydro-D-fructose					249:270	The sugar 1,5-anhydro-D-fructose	239:270	The sugar 1,5-anhydro-D-fructose (1,5-AF)	239:279	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	1	20	theme	glycogen	195:202	arg1	degradation					204:214	glycogen degradation	195:214	glycogen degradation by α-1,4-glucan lyase	195:236	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	8	21	theme	immunosorbent	1206:1218	arg1	assay					1220:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	0	22	from	production	35:44	arg1	1,5-anhydro-D-fructose					92:113	1,5-anhydro-D-fructose	92:113	1,5-anhydro-D-fructose	92:113	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	0	22	from	production	35:44	arg1	metabolite					127:136	a glycogen metabolite	116:136	a glycogen metabolite	116:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	11	23	theme	AF-AGEs	1413:1419	arg1	accumulation					1397:1408	intracellular accumulation	1383:1408	intracellular accumulation of AF-AGEs	1383:1419	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	8	24	theme	novel	1095:1099	arg1	antibody					1128:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	0	25	theme	Immunological	0:12	arg1	evidence					14:21	Immunological evidence	0:21	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.	0:137	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	9	26	theme	AF-AGEs	1235:1241	arg1	assay					1243:1247	Then an AF-AGEs assay	1227:1247	Then an AF-AGEs assay	1227:1247	Then an AF-AGEs assay was established using this immunopurified antibody.					
31308400	9	27	theme	immunopurified	1276:1289	arg1	antibody					1291:1298	this immunopurified antibody	1271:1298	this immunopurified antibody	1271:1298	Then an AF-AGEs assay was established using this immunopurified antibody.					
31308400	10	28	theme	human	1342:1346	arg1	samples					1365:1371	human and animal serum samples	1342:1371	human and animal serum samples	1342:1371	This assay was able to detect AF-AGEs in human and animal serum samples.					
31308400	3	29	theme	Glycation	384:392	arg1	reactions					394:402	Glycation reactions	384:402	Glycation reactions forming advanced glycation end-products (AGEs)	384:449	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	5	30	from	role	670:673	arg1	modification					696:707	protein modification	688:707	protein modification	688:707	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	7	31	theme	affinity	931:938	arg1	chromatography					940:953	affinity chromatography	931:953	affinity chromatography of anti-AF-AGE antiserum	931:978	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	1	32	theme	α-1,4-glucan	219:230	arg1	lyase					232:236	α-1,4-glucan lyase	219:236	α-1,4-glucan lyase	219:236	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	7	33	theme	4B	1009:1010	arg1	column					1012:1017	a Sepharose 4B column	997:1017	a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	997:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	4	34	theme	1,5-AF-derived	596:609	arg1	AF-AGEs					617:623	AF-AGEs	617:623	AF-AGEs	617:623	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	4	34	theme	1,5-AF-derived	596:609	arg1	AGEs					611:614	1,5-AF-derived AGEs	596:614	1,5-AF-derived AGEs (AF-AGEs)	596:624	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	5	35	theme	novel	723:727	arg1	AF-AGEs					748:754	a novel antibody targeting AF-AGEs	721:754	a novel antibody targeting AF-AGEs	721:754	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	0	36	from	1,5-anhydro-D-fructose	92:113	arg1	end-products					74:85	novel advanced glycation end-products	49:85	novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	49:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	0	36	from	1,5-anhydro-D-fructose	92:113	arg1	production					35:44	in vivo production	27:44	in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	27:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	2	37	theme	pathway	322:328	arg1	intermediate					301:312	the central intermediate	289:312	the central intermediate of this pathway	289:328	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	2	37	theme	pathway	322:328	arg1	1,5-anhydro-D-fructose					249:270	The sugar 1,5-anhydro-D-fructose	239:270	The sugar 1,5-anhydro-D-fructose (1,5-AF)	239:279	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	7	38	theme	antiserum	970:978	arg1	chromatography					940:953	affinity chromatography	931:953	affinity chromatography of anti-AF-AGE antiserum	931:978	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	3	39	theme	complications	487:499	arg1	development					472:482	the development	468:482	the development of complications of diabetes mellitus	468:520	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	5	40	theme	antibody	729:736	arg1	AF-AGEs					748:754	a novel antibody targeting AF-AGEs	721:754	a novel antibody targeting AF-AGEs	721:754	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	10	41	theme	serum	1359:1363	arg1	samples					1365:1371	human and animal serum samples	1342:1371	human and animal serum samples	1342:1371	This assay was able to detect AF-AGEs in human and animal serum samples.					
31308400	11	42	theme	intracellular	1383:1395	arg1	accumulation					1397:1408	intracellular accumulation	1383:1408	intracellular accumulation of AF-AGEs	1383:1419	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	5	43	theme	targeting	738:746	arg1	AF-AGEs					748:754	a novel antibody targeting AF-AGEs	721:754	a novel antibody targeting AF-AGEs	721:754	To clarify the role of 1,5-AF in protein modification, we created a novel antibody targeting AF-AGEs.					
31308400	12	44	theme	in	1530:1531	arg1	detection					1538:1546	in vivo detection	1530:1546	in vivo detection of AF-AGEs with a novel structural epitope	1530:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	0	45	theme	in vivo	27:33	arg1	production					35:44	in vivo production	27:44	in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	27:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	3	46	theme	glycation	421:429	arg1	end-products					431:442	advanced glycation end-products	412:442	advanced glycation end-products (AGEs)	412:449	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	3	46	theme	glycation	421:429	arg1	AGEs					445:448	AGEs	445:448	AGEs	445:448	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	12	47	theme	structural	1572:1581	arg1	epitope					1583:1589	a novel structural epitope	1564:1589	a novel structural epitope	1564:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	8	48	theme	immunopurified	1101:1114	arg1	antibody					1128:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody	1093:1135	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	0	49	theme	glycation	64:72	arg1	end-products					74:85	novel advanced glycation end-products	49:85	novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	49:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	3	50	from	development	472:482	arg1	important					455:463	important	455:463	important	455:463	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	3	51	theme	mellitus	513:520	arg1	complications					487:499	complications	487:499	complications of diabetes mellitus	487:520	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
31308400	0	52	theme	novel	49:53	arg1	end-products					74:85	novel advanced glycation end-products	49:85	novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	49:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	4	53	theme	cellular	569:576	arg1	damage					578:583	cellular damage	569:583	cellular damage	569:583	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	7	54	theme	carboxymethyl	1050:1062	arg1	lysine-BSA					1081:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	6	55	theme	serum	855:859	arg1	BSA					870:872	BSA	870:872	BSA	870:872	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	6	55	theme	serum	855:859	arg1	albumin					861:867	bovine serum albumin	848:867	bovine serum albumin (BSA)	848:873	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	2	56	theme	sugar	243:247	arg1	1,5-AF					273:278	1,5-AF	273:278	1,5-AF	273:278	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	2	56	theme	sugar	243:247	arg1	1,5-anhydro-D-fructose					249:270	The sugar 1,5-anhydro-D-fructose	239:270	The sugar 1,5-anhydro-D-fructose (1,5-AF)	239:279	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	2	56	theme	sugar	243:247	arg1	intermediate					301:312	the central intermediate	289:312	the central intermediate of this pathway	289:328	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	10	57	theme	animal	1352:1357	arg1	samples					1365:1371	human and animal serum samples	1342:1371	human and animal serum samples	1342:1371	This assay was able to detect AF-AGEs in human and animal serum samples.					
31308400	6	58	theme	bovine	848:853	arg1	BSA					870:872	BSA	870:872	BSA	870:872	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	6	58	theme	bovine	848:853	arg1	albumin					861:867	bovine serum albumin	848:867	bovine serum albumin (BSA)	848:873	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	1	59	theme	anhydrofructose	143:157	arg1	pathway					159:165	The anhydrofructose pathway	139:165	The anhydrofructose pathway	139:165	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	1	59	theme	anhydrofructose	143:157	arg1	pathway					183:189	an alternate pathway	170:189	an alternate pathway for glycogen degradation by α-1,4-glucan lyase	170:236	The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase.					
31308400	4	60	theme	intracellular	631:643	arg1	proteins					645:652	intracellular proteins	631:652	intracellular proteins	631:652	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	7	61	theme	N-	1047:1048	arg1	lysine-BSA					1081:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	1047:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	8	62	theme	enzyme-linked	1192:1204	arg1	assay					1220:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	a competitive enzyme-linked immunosorbent assay	1178:1224	A novel immunopurified anti-AF-AGE antibody was obtained and was characterized using a competitive enzyme-linked immunosorbent assay.					
31308400	7	63	theme	Sepharose	999:1007	arg1	column					1012:1017	a Sepharose 4B column	997:1017	a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA	997:1090	After immunizing rabbits with AF-AGEs-RSA, affinity chromatography of anti-AF-AGE antiserum was performed on a Sepharose 4B column coupled with AF-AGEs-BSA or N-(carboxymethyl)/N-(carboxyethyl)lysine-BSA.					
31308400	2	64	theme	physiological	339:351	arg1	role					353:356	its physiological role	335:356	its physiological role of in mammals	335:370	The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear.					
31308400	12	65	theme	AF-AGEs	1551:1557	arg1	detection					1538:1546	in vivo detection	1530:1546	in vivo detection of AF-AGEs with a novel structural epitope	1530:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	0	66	theme	end-products	74:85	arg1	production					35:44	in vivo production	27:44	in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	27:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	6	67	theme	Serum	757:761	arg1	albumin					763:769	Serum albumin	757:769	Serum albumin modified by AF-AGEs	757:789	Serum albumin modified by AF-AGEs was prepared by incubating rabbit serum albumin (RSA) or bovine serum albumin (BSA) with 1,5-AF.					
31308400	11	68	theme	HepG2	1485:1489	arg1	hepatocytes					1472:1482	cultured hepatocytes	1463:1482	cultured hepatocytes (HepG2 cells)	1463:1496	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	11	68	theme	HepG2	1485:1489	arg1	cells					1491:1495	HepG2 cells	1485:1495	HepG2 cells	1485:1495	Finally, intracellular accumulation of AF-AGEs was shown to be associated with damage to cultured hepatocytes (HepG2 cells).					
31308400	12	69	theme	novel	1566:1570	arg1	epitope					1583:1589	a novel structural epitope	1564:1589	a novel structural epitope	1564:1589	This is the first report about in vivo detection of AF-AGEs with a novel structural epitope.					
31308400	0	70	theme	advanced	55:62	arg1	end-products					74:85	novel advanced glycation end-products	49:85	novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite	49:136	Immunological evidence for in vivo production of novel advanced glycation end-products from 1,5-anhydro-D-fructose, a glycogen metabolite.					
31308400	4	71	link	1,5-AF-derived	596:609	arg1	AF-AGEs					617:623	AF-AGEs	617:623	AF-AGEs	617:623	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	4	71	link	1,5-AF-derived	596:609	arg1	AGEs					611:614	1,5-AF-derived AGEs	596:614	1,5-AF-derived AGEs (AF-AGEs)	596:624	We hypothesized that 1,5-AF may contribute to cellular damage by forming 1,5-AF-derived AGEs (AF-AGEs) with intracellular proteins.					
31308400	3	72	from	important	455:463	arg1	development					472:482	the development	468:482	the development of complications of diabetes mellitus	468:520	Glycation reactions forming advanced glycation end-products (AGEs) are important in the development of complications of diabetes mellitus.					
30804383	0	0	theme	Heparan	79:85	arg1	Glycosaminoglycans					95:112	Heparin-like and Heparan Sulfate Glycosaminoglycans	62:112	Glycosaminoglycans	95:112	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	5	1	theme	by-products	926:936	arg1	properties					899:908	Functional properties	888:908	Functional properties of the purified by-products	888:936	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	1	2	theme	large	243:247	arg1	amounts					249:255	large amounts	243:255	large amounts of waste by-products	243:276	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	1	2	theme	large	243:247	arg1	by-products					266:276	waste by-products	260:276	waste by-products	260:276	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	3	3	theme	functional	495:504	arg1	characterisation					506:521	structural and functional characterisation	480:521	structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	480:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	3	theme	functional	495:504	arg1	purification					466:477	the first purification	456:477	the first purification	456:477	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	0	4	theme	Glycosaminoglycans	95:112	arg1	Source					52:57	a Diverse Source	42:57	a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans	42:112	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	2	5	with	glycosaminoglycans	359:376	arg1	potential					428:436	pharmaceutical potential	413:436	pharmaceutical potential	413:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	7	6	theme	future	1457:1462	arg1	studies					1483:1489	future structure-activity studies	1457:1489	future structure-activity studies	1457:1489	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	3	7	theme	HS	538:539	arg1	polysaccharides					541:555	Hp-like and HS polysaccharides	526:555	Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	526:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	1	8	theme	Global	115:120	arg1	production					122:131	Global production	115:131	Global production of pharmaceutical heparin (Hp)	115:162	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	0	9	theme	Sulfate	87:93	arg1	Glycosaminoglycans					95:112	Heparin-like and Heparan Sulfate Glycosaminoglycans	62:112	Glycosaminoglycans	95:112	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	6	10	theme	factor	1210:1215	arg1	receptor					1217:1224	fibroblast growth factor receptor	1192:1224	fibroblast growth factor receptor signalling	1192:1235	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	7	11	theme	natural	1553:1559	arg1	products					1561:1568	these important natural products	1537:1568	these important natural products	1537:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	3	12	from	characterisation	506:521	arg1	fractions					588:596	the four major by-product fractions	562:596	the four major by-product fractions of standard heparin production	562:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	13	theme	polysaccharides	541:555	arg1	characterisation					506:521	structural and functional characterisation	480:521	structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	480:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	13	theme	polysaccharides	541:555	arg1	purification					466:477	the first purification	456:477	the first purification	456:477	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	1	14	theme	waste	260:264	arg1	by-products					266:276	waste by-products	260:276	waste by-products	260:276	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	4	15	dep	differentiate	760:772	arg1	content					845:851	sulfated disaccharide content	823:851	sulfated disaccharide content	823:851	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	4	15	dep	differentiate	760:772	arg1	sulfation					809:817	sulfation	809:817	sulfation	809:817	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	7	16	theme	Hp-like	1393:1399	arg1	source					1362:1367	an untapped and economical source	1335:1367	an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1335:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	3	17	theme	Hp-like	526:532	arg1	polysaccharides					541:555	Hp-like and HS polysaccharides	526:555	Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	526:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	4	18	theme	composition	674:684	arg1	analysis					686:693	disaccharide composition analysis	661:693	disaccharide composition analysis	661:693	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	2	19	with	Hp-like	308:314	arg1	potential					428:436	pharmaceutical potential	413:436	pharmaceutical potential	413:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	20	with	entities	399:406	arg1	potential					428:436	pharmaceutical potential	413:436	pharmaceutical potential	413:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	6	21	theme	cell	1169:1172	arg1	proliferation					1174:1186	cell proliferation	1169:1186	cell proliferation	1169:1186	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	4	22	theme	reduced	801:807	arg1	sulfation					809:817	sulfation	809:817	sulfation	809:817	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	4	23	theme	disaccharide	661:672	arg1	analysis					686:693	disaccharide composition analysis	661:693	disaccharide composition analysis	661:693	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	5	24	theme	specific	1065:1072	arg1	inhibition					1074:1083	significantly lower global and specific inhibition	1034:1083	significantly lower global and specific inhibition of the coagulation pathway than Hp	1034:1118	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	1	25	theme	heparin	151:157	arg1	production					122:131	Global production	115:131	Global production of pharmaceutical heparin (Hp)	115:162	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	7	26	theme	collective	1286:1295	arg1	analyses					1297:1304	These collective analyses	1280:1304	These collective analyses	1280:1304	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	5	27	theme	anticoagulant	971:983	arg1	profiles					985:992	distinct anticoagulant profiles	962:992	distinct anticoagulant profiles	962:992	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	7	28	theme	untapped	1338:1345	arg1	source					1362:1367	an untapped and economical source	1335:1367	an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1335:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	2	29	theme	other	353:357	arg1	glycosaminoglycans					359:376	other glycosaminoglycans	353:376	other glycosaminoglycans	353:376	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	7	30	theme	economical	1351:1360	arg1	source					1362:1367	an untapped and economical source	1335:1367	an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1335:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	1	31	theme	by-products	266:276	arg1	amounts					249:255	large amounts	243:255	large amounts of waste by-products	243:276	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	1	31	theme	by-products	266:276	arg1	by-products					266:276	waste by-products	260:276	waste by-products	260:276	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	4	32	theme	characteristics	738:752	arg1	range					718:722	a range	716:722	a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct	716:885	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	3	33	theme	major	571:575	arg1	fractions					588:596	the four major by-product fractions	562:596	the four major by-product fractions of standard heparin production	562:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	7	34	theme	HS	1405:1406	arg1	polysaccharides					1408:1422	structurally-diverse Hp-like and HS polysaccharides	1372:1422	polysaccharides	1408:1422	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	0	35	theme	Production	23:32	arg1	By-Products					0:10	By-Products	0:10	By-Products of Heparin Production	0:32	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	5	36	theme	lower	1048:1052	arg1	inhibition					1074:1083	significantly lower global and specific inhibition	1034:1083	significantly lower global and specific inhibition of the coagulation pathway than Hp	1034:1118	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	5	37	theme	distinct	962:969	arg1	profiles					985:992	distinct anticoagulant profiles	962:992	distinct anticoagulant profiles	962:992	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	2	38	theme	sulfated	299:306	arg1	HS					337:338	HS	337:338	HS	337:338	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	38	theme	sulfated	299:306	arg1	Hp-like					308:314	lower sulfated Hp-like and heparan sulfate	293:334	Hp-like	308:314	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	3	39	from	fractions	588:596	arg1	polysaccharides					541:555	Hp-like and HS polysaccharides	526:555	Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	526:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	39	from	fractions	588:596	arg1	characterisation					506:521	structural and functional characterisation	480:521	structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	480:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	39	from	fractions	588:596	arg1	purification					466:477	the first purification	456:477	the first purification	456:477	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	1	40	theme	production	187:196	arg1	process					198:204	the production process	183:204	the production process from raw mucosal material	183:230	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	0	41	theme	Heparin	15:21	arg1	Production					23:32	Heparin Production	15:32	Heparin Production	15:32	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	2	42	with	sulfate	328:334	arg1	potential					428:436	pharmaceutical potential	413:436	pharmaceutical potential	413:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	43	theme	lower	293:297	arg1	HS					337:338	HS	337:338	HS	337:338	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	43	theme	lower	293:297	arg1	Hp-like					308:314	lower sulfated Hp-like and heparan sulfate	293:334	Hp-like	308:314	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	7	44	theme	biomedical	1510:1519	arg1	applications					1521:1532	new biomedical applications	1506:1532	new biomedical applications of these important natural products	1506:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	2	45	theme	bioactive	389:397	arg1	which					379:383	which	379:383	which	379:383	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	45	theme	bioactive	389:397	arg1	entities					399:406	bioactive entities	389:406	bioactive entities with pharmaceutical potential	389:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	entities					399:406	bioactive entities	389:406	bioactive entities with pharmaceutical potential	389:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	sulfate					328:334	lower sulfated Hp-like and heparan sulfate	293:334	sulfate	328:334	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	HS					337:338	HS	337:338	HS	337:338	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg1	These					279:283	These	279:283	These	279:283	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	Hp-like					308:314	lower sulfated Hp-like and heparan sulfate	293:334	Hp-like	308:314	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	glycosaminoglycans					359:376	other glycosaminoglycans	353:376	other glycosaminoglycans	353:376	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	2	46	contain	contain	285:291	arg2	which					379:383	which	379:383	which	379:383	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	5	47	theme	global	1054:1059	arg1	inhibition					1074:1083	significantly lower global and specific inhibition	1034:1083	significantly lower global and specific inhibition of the coagulation pathway than Hp	1034:1118	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	6	48	theme	minor	1248:1252	arg1	differences					1254:1264	only minor differences	1243:1264	only minor differences between them	1243:1277	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	6	49	theme	receptor	1217:1224	arg1	signalling					1226:1235	fibroblast growth factor receptor signalling	1192:1235	fibroblast growth factor receptor signalling	1192:1235	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	7	50	theme	polysaccharides	1408:1422	arg1	source					1362:1367	an untapped and economical source	1335:1367	an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1335:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	7	51	with	Hp-like	1393:1399	arg1	potential					1433:1441	the potential	1429:1441	the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1429:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	2	52	theme	pharmaceutical	413:426	arg1	potential					428:436	pharmaceutical potential	413:436	pharmaceutical potential	413:436	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	7	53	theme	structure-activity	1464:1481	arg1	studies					1483:1489	future structure-activity studies	1457:1489	future structure-activity studies	1457:1489	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	1	54	theme	raw	211:213	arg1	material					223:230	raw mucosal material	211:230	raw mucosal material	211:230	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	3	55	theme	by-product	577:586	arg1	fractions					588:596	the four major by-product fractions	562:596	the four major by-product fractions of standard heparin production	562:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	7	56	theme	products	1561:1568	arg1	applications					1521:1532	new biomedical applications	1506:1532	new biomedical applications of these important natural products	1506:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	4	57	theme	structural	727:736	arg1	characteristics					738:752	structural characteristics	727:752	structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct	727:885	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	0	58	theme	Diverse	44:50	arg1	Source					52:57	a Diverse Source	42:57	a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans	42:112	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	7	59	theme	new	1506:1508	arg1	applications					1521:1532	new biomedical applications	1506:1532	new biomedical applications of these important natural products	1506:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	3	60	theme	structural	480:489	arg1	characterisation					506:521	structural and functional characterisation	480:521	structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	480:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	60	theme	structural	480:489	arg1	purification					466:477	the first purification	456:477	the first purification	456:477	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	4	61	theme	disaccharide	832:843	arg1	content					845:851	sulfated disaccharide content	823:851	sulfated disaccharide content	823:851	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	7	62	with	polysaccharides	1408:1422	arg1	potential					1433:1441	the potential	1429:1441	the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products	1429:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	5	63	theme	coagulation	1092:1102	arg1	pathway					1104:1110	the coagulation pathway	1088:1110	the coagulation pathway	1088:1110	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	4	64	theme	sulfated	823:830	arg1	content					845:851	sulfated disaccharide content	823:851	sulfated disaccharide content	823:851	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	3	65	theme	standard	601:608	arg1	production					618:627	standard heparin production	601:627	standard heparin production	601:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	1	66	theme	pharmaceutical	136:149	arg1	Hp					160:161	Hp	160:161	Hp	160:161	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	1	66	theme	pharmaceutical	136:149	arg1	heparin					151:157	pharmaceutical heparin	136:157	pharmaceutical heparin (Hp)	136:162	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	6	67	theme	growth	1203:1208	arg1	receptor					1217:1224	fibroblast growth factor receptor	1192:1224	fibroblast growth factor receptor signalling	1192:1235	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	1	68	theme	mucosal	215:221	arg1	material					223:230	raw mucosal material	211:230	raw mucosal material	211:230	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	1	69	from	material	223:230	arg1	process					198:204	the production process	183:204	the production process from raw mucosal material	183:230	Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products.					
30804383	5	70	theme	pathway	1104:1110	arg1	inhibition					1074:1083	significantly lower global and specific inhibition	1034:1083	significantly lower global and specific inhibition of the coagulation pathway than Hp	1034:1118	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	7	71	theme	important	1543:1551	arg1	products					1561:1568	these important natural products	1537:1568	these important natural products	1537:1568	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	2	72	theme	heparan	320:326	arg1	sulfate					328:334	lower sulfated Hp-like and heparan sulfate	293:334	sulfate	328:334	These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential.					
30804383	3	73	theme	first	460:464	arg1	characterisation					506:521	structural and functional characterisation	480:521	structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production	480:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	3	73	theme	first	460:464	arg1	purification					466:477	the first purification	456:477	the first purification	456:477	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	0	74	theme	Heparin-like	62:73	arg1	Glycosaminoglycans					95:112	Heparin-like and Heparan Sulfate Glycosaminoglycans	62:112	Glycosaminoglycans	95:112	By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.					
30804383	4	75	theme	by-products	646:656	arg1	Analysis					630:637	Analysis	630:637	Analysis of the by-products by disaccharide composition analysis and NMR	630:701	Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct.					
30804383	3	76	theme	heparin	610:616	arg1	production					618:627	standard heparin production	601:627	standard heparin production	601:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	7	77	theme	structurally-diverse	1372:1391	arg1	Hp-like					1393:1399	structurally-diverse Hp-like and HS polysaccharides	1372:1422	Hp-like	1393:1399	These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.					
30804383	5	78	theme	different	997:1005	arg1	assays					1007:1012	different assays	997:1012	different assays	997:1012	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	5	79	theme	Functional	888:897	arg1	properties					899:908	Functional properties	888:908	Functional properties of the purified by-products	888:936	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
30804383	6	80	theme	fibroblast	1192:1201	arg1	receptor					1217:1224	fibroblast growth factor receptor	1192:1224	fibroblast growth factor receptor signalling	1192:1235	The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them.					
30804383	3	81	theme	production	618:627	arg1	fractions					588:596	the four major by-product fractions	562:596	the four major by-product fractions of standard heparin production	562:627	Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production.					
30804383	5	82	theme	purified	917:924	arg1	by-products					926:936	the purified by-products	913:936	the purified by-products	913:936	Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp.					
29561006	2	0	with	polysaccharide	292:305	arg1	content					326:332	a total sugar content	312:332	a total sugar content of 92.51%	312:342	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	3	1	theme	chromatography	422:435	arg1	spectrum					445:452	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	8	2	theme	anti-aging	1382:1391	arg1	abilities					1393:1401	significant antioxidant and anti-aging abilities	1354:1401	significant antioxidant and anti-aging abilities	1354:1401	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	4	3	with	d-glucose	634:642	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	5	4	theme	→1	954:955	arg1	chains					944:949	side chains	939:949	side chains of →1	939:955	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	5	theme	-α-d-GlcpA-	1013:1023	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	6	theme	NMR	867:869	arg1	analysis					871:878	NMR analysis	867:878	NMR analysis	867:878	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	4	7	with	d-xylose	624:631	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	8	8	theme	anti-aging	1315:1324	arg1	analyses					1326:1333	Anti-oxidative and anti-aging analyses	1296:1333	Anti-oxidative and anti-aging analyses	1296:1333	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	1	9	theme	Sephadex	214:221	arg1	G-200					223:227	Sephadex G-200	214:227	Sephadex G-200	214:227	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	0	10	theme	rose	90:93	arg1	buds					95:98	rose buds	90:98	rose buds	90:98	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	5	11	theme	Smith	831:835	arg1	degradation					837:847	Smith degradation	831:847	Smith degradation	831:847	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	12	dep	has	899:901	arg1	4→					1047:1048	4→	1047:1048	4→	1047:1048	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	3	13	theme	high-performance	390:405	arg1	HPGPC					438:442	HPGPC	438:442	HPGPC	438:442	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	3	13	theme	high-performance	390:405	arg1	chromatography					422:435	its high-performance gel-permeation chromatography	386:435	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	5	14	dep	such	779:782	arg1	as					784:785	as	784:785	as	784:785	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	2	15	theme	total	314:318	arg1	content					326:332	a total sugar content	312:332	a total sugar content of 92.51%	312:342	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	4	16	with	d-galactose	645:655	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	7	17	contain	have	1211:1214	arg1	R-PL					1197:1200	R-PL	1197:1200	R-PL	1197:1200	The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons.					
29561006	7	17	contain	have	1211:1214	arg2	conformation					1233:1244	a triple-helical conformation	1216:1244	a triple-helical conformation	1216:1244	The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons.					
29561006	4	18	theme	d-glucuronic	683:694	arg1	acid					696:699	d-glucuronic acid	683:699	d-glucuronic acid	683:699	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	1	19	theme	rose	156:159	arg1	buds					161:164	rose buds	156:164	rose buds	156:164	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	0	20	theme	chemical	4:11	arg1	structure					13:21	chemical structure	4:21	chemical structure	4:21	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	5	21	theme	side	939:942	arg1	chains					944:949	side chains	939:949	side chains of →1	939:955	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	6	22	theme	electron	1139:1146	arg1	SEM					1160:1162	SEM	1160:1162	SEM	1160:1162	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	6	22	theme	electron	1139:1146	arg1	microscopy					1148:1157	scanning electron microscopy	1130:1157	scanning electron microscopy (SEM) analysis	1130:1172	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	3	23	theme	homogeneous	479:489	arg1	R-PL					469:472	R-PL	469:472	R-PL	469:472	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	3	23	theme	homogeneous	479:489	arg1	polysaccharide					491:504	a homogeneous polysaccharide	477:504	a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da	477:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	4	24	with	d-arabinose	611:621	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	4	25	with	acid	696:699	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	7	26	theme	triple-helical	1218:1231	arg1	conformation					1233:1244	a triple-helical conformation	1216:1244	a triple-helical conformation	1216:1244	The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons.					
29561006	2	27	theme	total	253:257	arg1	yield					259:263	a total yield	251:263	a total yield of 1.90%	251:272	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	5	28	theme	-α-d-GalpA-	1035:1045	arg1	backbone					905:912	a backbone	903:912	a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	903:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	6	29	theme	microscopy	1148:1157	arg1	analysis					1165:1172	scanning electron microscopy (SEM) analysis	1130:1172	scanning electron microscopy (SEM) analysis	1130:1172	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	8	30	theme	significant	1354:1364	arg1	abilities					1393:1401	significant antioxidant and anti-aging abilities	1354:1401	significant antioxidant and anti-aging abilities	1354:1401	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	8	31	contain	has	1350:1352	arg1	R-PL					1345:1348	R-PL	1345:1348	R-PL	1345:1348	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	8	31	contain	has	1350:1352	arg2	abilities					1393:1401	significant antioxidant and anti-aging abilities	1354:1401	significant antioxidant and anti-aging abilities	1354:1401	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	5	32	theme	acid	815:818	arg1	oxidation					820:828	periodic acid oxidation	806:828	periodic acid oxidation	806:828	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	4	33	theme	4.6 	723:726	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	1	34	theme	hot	169:171	arg1	water					173:177	hot water	169:177	hot water (80 °C) extraction	169:196	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	1	34	theme	hot	169:171	arg1	°C					183:184	80 °C	180:184	80 °C	180:184	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	2	35	theme	%	342:342	arg1	content					326:332	a total sugar content	312:332	a total sugar content of 92.51%	312:342	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	5	36	theme	-β-d-Araf-	977:986	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	37	theme	periodic	806:813	arg1	oxidation					820:828	periodic acid oxidation	806:828	periodic acid oxidation	806:828	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	4	38	theme	d-galacturonic	658:671	arg1	acid					673:676	d-galacturonic acid	658:676	d-galacturonic acid	658:676	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	1	39	theme	water	173:177	arg1	extraction					187:196	hot water (80 °C) extraction	169:196	hot water (80 °C) extraction	169:196	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	3	40	theme	gel-permeation	407:420	arg1	HPGPC					438:442	HPGPC	438:442	HPGPC	438:442	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	3	40	theme	gel-permeation	407:420	arg1	chromatography					422:435	its high-performance gel-permeation chromatography	386:435	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	6	41	theme	morphological	1055:1067	arg1	characteristics					1069:1083	Its morphological characteristics	1051:1083	Its morphological characteristics	1051:1083	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	7	42	theme	twisted	1279:1285	arg1	ribbons					1287:1293	twisted ribbons	1279:1293	twisted ribbons	1279:1293	The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons.					
29561006	3	43	theme	molecular	513:521	arg1	weight					523:528	a molecular weight	511:528	a molecular weight of 7.727 × 105 Da	511:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	5	44	with	6→	931:932	arg1	chains					944:949	side chains	939:949	side chains of →1	939:955	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	4	45	theme	composition	564:574	arg1	analysis					576:583	Monosaccharide composition analysis	549:583	Monosaccharide composition analysis	549:583	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	0	46	theme	anti-aging	27:36	arg1	bioactivity					38:48	anti-aging bioactivity	27:48	anti-aging bioactivity	27:48	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	4	47	with	acid	673:676	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	3	48	theme	Da	545:546	arg1	weight					523:528	a molecular weight	511:528	a molecular weight of 7.727 × 105 Da	511:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	8	49	theme	antioxidant	1366:1376	arg1	abilities					1393:1401	significant antioxidant and anti-aging abilities	1354:1401	significant antioxidant and anti-aging abilities	1354:1401	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	2	50	theme	sugar	320:324	arg1	content					326:332	a total sugar content	312:332	a total sugar content of 92.51%	312:342	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	5	51	contain	has	899:901	arg1	R-PL					894:897	R-PL	894:897	R-PL	894:897	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	51	contain	has	899:901	arg2	backbone					905:912	a backbone	903:912	a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	903:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	52	theme	-β-d-Galp-	957:966	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	6	53	theme	red	1110:1112	arg1	experiments					1114:1124	Congo red experiments	1104:1124	Congo red experiments	1104:1124	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	5	54	theme	-α-d-Glcp-	920:929	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	1	55	theme	acid	104:107	arg1	polysaccharide					109:122	An acid polysaccharide	101:122	An acid polysaccharide	101:122	An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200.					
29561006	4	56	dep	ratio	714:718	arg1	 1 					748:750	 1 	748:750	 1 	748:750	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	4	56	dep	ratio	714:718	arg1	 1.4 					728:732	 1.4 	728:732	 1.4 	728:732	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	4	56	dep	ratio	714:718	arg1	 4.81 					741:746	 4.81 	741:746	 4.81 	741:746	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	4	56	dep	ratio	714:718	arg1	 5.22 					734:739	 5.22 	734:739	 5.22 	734:739	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	4	56	dep	ratio	714:718	arg1	 1.86					752:756	 1.86	752:756	 1.86	752:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	8	57	theme	Anti-oxidative	1296:1309	arg1	analyses					1326:1333	Anti-oxidative and anti-aging analyses	1296:1333	Anti-oxidative and anti-aging analyses	1296:1333	Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.					
29561006	2	58	theme	%	272:272	arg1	yield					259:263	a total yield	251:263	a total yield of 1.90%	251:272	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	4	59	theme	molar	708:712	arg1	ratio					714:718	a molar ratio	706:718	a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86	706:756	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	3	60	theme	×	539:539	arg1	Da					545:546	7.727 × 105 Da	533:546	7.727 × 105 Da	533:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	3	61	from	peak	378:381	arg1	spectrum					445:452	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	its high-performance gel-permeation chromatography (HPGPC) spectrum	386:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	5	62	theme	FTIR	787:790	arg1	spectroscopy					792:803	FTIR spectroscopy	787:803	FTIR spectroscopy	787:803	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	63	theme	-α-d-Xylp-	995:1004	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	0	64	dep	structure	13:21	arg1	The					0:2	The	0:2	The	0:2	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	5	65	theme	Structure	759:767	arg1	analysis					769:776	Structure analysis	759:776	Structure analysis	759:776	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	66	dep	-α-d-Glcp-	920:929	arg1	6→					931:932	6→	931:932	6→ with side chains of →1	931:955	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	0	67	theme	polysaccharide	61:74	arg1	structure					13:21	chemical structure	4:21	chemical structure	4:21	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	0	67	theme	polysaccharide	61:74	arg1	bioactivity					38:48	anti-aging bioactivity	27:48	anti-aging bioactivity	27:48	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	4	68	theme	Monosaccharide	549:562	arg1	analysis					576:583	Monosaccharide composition analysis	549:583	Monosaccharide composition analysis	549:583	Monosaccharide composition analysis shows that it consists of d-arabinose, d-xylose, d-glucose, d-galactose, d-galacturonic acid, and d-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86.					
29561006	6	69	theme	scanning	1130:1137	arg1	SEM					1160:1162	SEM	1160:1162	SEM	1160:1162	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	6	69	theme	scanning	1130:1137	arg1	microscopy					1148:1157	scanning electron microscopy	1130:1157	scanning electron microscopy (SEM) analysis	1130:1172	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
29561006	3	70	theme	sharp	372:376	arg1	peak					378:381	A single and symmetrically sharp peak	345:381	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum	345:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	2	71	theme	pure	287:290	arg1	polysaccharide					292:305	a highly pure polysaccharide	278:305	a highly pure polysaccharide with a total sugar content of 92.51%	278:342	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	2	71	theme	pure	287:290	arg1	R-PL					230:233	R-PL	230:233	R-PL	230:233	R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%.					
29561006	3	72	theme	single	347:352	arg1	peak					378:381	A single and symmetrically sharp peak	345:381	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum	345:452	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	5	73	theme	4→	1006:1007	arg1	-α-d-GalpA-					1035:1045	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	-α-d-GalpA-	1035:1045	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	0	74	theme	acid	56:59	arg1	polysaccharide					61:74	an acid polysaccharide	53:74	an acid polysaccharide	53:74	The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds.					
29561006	3	75	theme	7.727	533:537	arg1	Da					545:546	7.727 × 105 Da	533:546	7.727 × 105 Da	533:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	5	76	dep	-α-d-GalpA-	1035:1045	arg1	→1					1010:1011	→1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-	917:1045	→1	1010:1011	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	76	dep	-α-d-GalpA-	1035:1045	arg1	→1					1032:1033	→1	1032:1033	→1	1032:1033	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	5	76	dep	-α-d-GalpA-	1035:1045	arg1	2→					1025:1026	2→	1025:1026	2→	1025:1026	Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-d-Glcp-(6→ with side chains of →1)-β-d-Galp-(4,6→, →1)-β-d-Araf-(5→, →1)-α-d-Xylp-(4→, →1)-α-d-GlcpA-(2→ and →1)-α-d-GalpA-(4→.					
29561006	3	77	with	polysaccharide	491:504	arg1	weight					523:528	a molecular weight	511:528	a molecular weight of 7.727 × 105 Da	511:546	A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da.					
29561006	6	78	theme	Congo	1104:1108	arg1	experiments					1114:1124	Congo red experiments	1104:1124	Congo red experiments	1104:1124	Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis.					
31812968	0	0	theme	Dysphagia	75:83	arg1	Management					61:70	the Management	57:70	the Management of Dysphagia	57:83	Lack of Standardization in Commercial Thickeners Used in the Management of Dysphagia.					
31812968	6	1	dep	Products	988:995	arg1	Products					988:995	Products B, C, and E	988:1007	RESULTS Products B, C, and E	980:1007	RESULTS Products B, C, and E presented similar composition.					
31812968	6	1	dep	Products	988:995	arg1	C					1000:1000	C	1000:1000	C	1000:1000	RESULTS Products B, C, and E presented similar composition.					
31812968	6	1	dep	Products	988:995	arg1	E					1007:1007	E	1007:1007	E	1007:1007	RESULTS Products B, C, and E presented similar composition.					
31812968	6	1	dep	Products	988:995	arg1	B					997:997	B	997:997	B	997:997	RESULTS Products B, C, and E presented similar composition.					
31812968	5	2	theme	intermediate	901:912	arg1	level					925:929	level 2	925:931	level 2	925:931	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	5	2	theme	intermediate	901:912	arg1	viscosity					914:922	intermediate viscosity	901:922	intermediate viscosity (level 2)	901:932	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	4	3	from	available	622:630	arg1	Brazil					635:640	Brazil	635:640	Brazil	635:640	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	10	4	theme	viscosity	1435:1443	arg1	classifications					1445:1459	viscosity classifications	1435:1459	viscosity classifications provided by National Dysphagia Diet and IDDSI	1435:1505	There was no established correlation between viscosity classifications provided by National Dysphagia Diet and IDDSI.					
31812968	2	5	theme	food	344:347	arg1	preparation					320:330	the proper preparation	309:330	the proper preparation of modified food	309:347	Commercial thickeners are prescribed for these patients to change food viscosity and may alter the proper preparation of modified food.					
31812968	4	6	from	brands	615:620	arg1	thickeners					589:598	all thickeners	585:598	all thickeners	585:598	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	0	7	from	Lack	0:3	arg1	Thickeners					38:47	Commercial Thickeners	27:47	Commercial Thickeners	27:47	Lack of Standardization in Commercial Thickeners Used in the Management of Dysphagia.					
31812968	4	8	theme	Initiative	773:782	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	4	9	theme	IDDSI	785:789	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	2	10	theme	food	280:283	arg1	viscosity					285:293	food viscosity	280:293	food viscosity	280:293	Commercial thickeners are prescribed for these patients to change food viscosity and may alter the proper preparation of modified food.					
31812968	3	11	theme	nutritional	489:499	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	8	12	theme	Viscosity	1162:1170	arg1	tests					1172:1176	Viscosity tests	1162:1176	Viscosity tests	1162:1176	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	3	13	theme	information	501:511	arg1	viscosity					518:526	viscosity	518:526	viscosity of 7 commercial thickeners	518:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	terminology					390:400	employed terminology	381:400	employed terminology	381:400	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	amount					441:446	recommended amount	429:446	recommended amount	429:446	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	weight					452:457	weight	452:457	weight	452:457	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	instructions					415:426	preparation instructions	403:426	preparation instructions	403:426	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	composition					368:378	composition	368:378	composition	368:378	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	13	theme	information	501:511	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	7	14	theme	preparation	1088:1098	arg1	procedure					1100:1108	preparation procedure	1088:1108	preparation procedure	1088:1108	Manufacturer's information about the amount and preparation procedure, time, temperature, and base liquid was incomplete.					
31812968	5	15	theme	mineral	825:831	arg1	water					833:837	mineral water	825:837	mineral water (25°C)	825:844	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	5	15	theme	mineral	825:831	arg1	25°C					840:843	25°C	840:843	25°C	840:843	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	3	16	theme	commercial	533:542	arg1	thickeners					544:553	7 commercial thickeners	531:553	7 commercial thickeners	531:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	1	17	theme	eating	169:174	arg1	pleasure					176:183	eating pleasure	169:183	eating pleasure	169:183	INTRODUCTION Oral feeding safety is necessary to provide nutrition, hydration, and eating pleasure for patients with dysphagia.					
31812968	4	18	theme	different	605:613	arg1	brands					615:620	different brands	605:620	different brands available in Brazil	605:640	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	10	19	theme	National	1473:1480	arg1	Diet					1492:1495	National Dysphagia Diet	1473:1495	National Dysphagia Diet	1473:1495	There was no established correlation between viscosity classifications provided by National Dysphagia Diet and IDDSI.					
31812968	10	20	theme	established	1403:1413	arg1	correlation					1415:1425	no established correlation	1400:1425	no established correlation between viscosity classifications provided by National Dysphagia Diet and IDDSI	1400:1505	There was no established correlation between viscosity classifications provided by National Dysphagia Diet and IDDSI.					
31812968	3	21	theme	thickeners	544:553	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	21	theme	thickeners	544:553	arg1	viscosity					518:526	viscosity	518:526	viscosity of 7 commercial thickeners	518:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	21	theme	thickeners	544:553	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	22	theme	employed	381:388	arg1	terminology					390:400	employed terminology	381:400	employed terminology	381:400	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	4	23	theme	Standardization	757:771	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	3	24	theme	recommended	429:439	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	24	theme	recommended	429:439	arg1	viscosity					518:526	viscosity	518:526	viscosity of 7 commercial thickeners	518:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	24	theme	recommended	429:439	arg1	amount					441:446	recommended amount	429:446	recommended amount	429:446	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	24	theme	recommended	429:439	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	9	25	from	differences	1315:1325	arg1	viscosity					1345:1353	viscosity	1345:1353	viscosity	1345:1353	CONCLUSIONS The study showed differences in components and viscosity, beyond the lack of label details.					
31812968	9	25	from	differences	1315:1325	arg1	components					1330:1339	components	1330:1339	components	1330:1339	CONCLUSIONS The study showed differences in components and viscosity, beyond the lack of label details.					
31812968	7	26	theme	liquid	1139:1144	arg1	base					1134:1137	base liquid	1134:1144	base liquid	1134:1144	Manufacturer's information about the amount and preparation procedure, time, temperature, and base liquid was incomplete.					
31812968	4	27	theme	International	728:740	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	4	28	theme	Diet	752:755	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	8	29	theme	thickener	1192:1200	arg1	C					1202:1202	thickener C	1192:1202	thickener C	1192:1202	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	9	30	dep	CONCLUSIONS	1286:1296	arg1	showed					1308:1313	showed	1308:1313	showed	1308:1313	CONCLUSIONS The study showed differences in components and viscosity, beyond the lack of label details.					
31812968	1	31	theme	Oral	99:102	arg1	INTRODUCTION					86:97	INTRODUCTION Oral	86:102	INTRODUCTION Oral feeding safety	86:117	INTRODUCTION Oral feeding safety is necessary to provide nutrition, hydration, and eating pleasure for patients with dysphagia.					
31812968	3	32	theme	measuring	471:479	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	0	33	theme	Standardization	8:22	arg1	Lack					0:3	Lack	0:3	Lack of Standardization in Commercial Thickeners	0:47	Lack of Standardization in Commercial Thickeners Used in the Management of Dysphagia.					
31812968	9	34	dep	details	1381:1387	arg1	label					1375:1379	label	1375:1379	label	1375:1379	CONCLUSIONS The study showed differences in components and viscosity, beyond the lack of label details.					
31812968	3	35	theme	viscosity	518:526	arg1	viscosity					518:526	viscosity	518:526	viscosity of 7 commercial thickeners	518:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	terminology					390:400	employed terminology	381:400	employed terminology	381:400	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	amount					441:446	recommended amount	429:446	recommended amount	429:446	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	weight					452:457	weight	452:457	weight	452:457	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	instructions					415:426	preparation instructions	403:426	preparation instructions	403:426	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	composition					368:378	composition	368:378	composition	368:378	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	35	theme	viscosity	518:526	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	viscosity					518:526	viscosity	518:526	viscosity of 7 commercial thickeners	518:553	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	terminology					390:400	employed terminology	381:400	employed terminology	381:400	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	amount					441:446	recommended amount	429:446	recommended amount	429:446	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	weight					452:457	weight	452:457	weight	452:457	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	information					501:511	nutritional information	489:511	nutritional information	489:511	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	instructions					415:426	preparation instructions	403:426	preparation instructions	403:426	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	composition					368:378	composition	368:378	composition	368:378	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	3	36	theme	spoons	481:486	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	8	37	theme	desired	1261:1267	arg1	level					1279:1283	the desired viscosity level	1257:1283	the desired viscosity level	1257:1283	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	4	38	theme	G.	654:655	arg1	Products					657:664	G. Products	654:664	G. Products	654:664	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	0	39	theme	Commercial	27:36	arg1	Thickeners					38:47	Commercial Thickeners	27:47	Commercial Thickeners	27:47	Lack of Standardization in Commercial Thickeners Used in the Management of Dysphagia.					
31812968	1	40	with	patients	189:196	arg1	dysphagia					203:211	dysphagia	203:211	dysphagia	203:211	INTRODUCTION Oral feeding safety is necessary to provide nutrition, hydration, and eating pleasure for patients with dysphagia.					
31812968	3	41	theme	provided	462:469	arg1	spoons					481:486	provided measuring spoons	462:486	provided measuring spoons	462:486	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	8	42	theme	level	1279:1283	arg1	results					1242:1248	results	1242:1248	results out of the desired viscosity level	1242:1283	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	5	43	theme	thickener	869:877	arg1	thickener					869:877	thickener	869:877	thickener	869:877	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	5	43	theme	thickener	869:877	arg1	amount					859:864	the amount	855:864	the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions	855:977	Samples were prepared with mineral water (25°C) and with the amount of thickener recommended to obtain intermediate viscosity (level 2) according to the manufacturer's instructions.					
31812968	2	44	theme	Commercial	214:223	arg1	thickeners					225:234	Commercial thickeners	214:234	Commercial thickeners	214:234	Commercial thickeners are prescribed for these patients to change food viscosity and may alter the proper preparation of modified food.					
31812968	2	45	theme	modified	335:342	arg1	food					344:347	modified food	335:347	modified food	335:347	Commercial thickeners are prescribed for these patients to change food viscosity and may alter the proper preparation of modified food.					
31812968	3	46	theme	preparation	403:413	arg1	instructions					415:426	preparation instructions	403:426	preparation instructions	403:426	OBJECTIVE Analyze composition, employed terminology, preparation instructions, recommended amount and weight of provided measuring spoons, nutritional information, and viscosity of 7 commercial thickeners.					
31812968	8	47	theme	viscosity	1269:1277	arg1	level					1279:1283	the desired viscosity level	1257:1283	the desired viscosity level	1257:1283	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	4	48	theme	Dysphagia	742:750	arg1	test					792:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	the International Dysphagia Diet Standardization Initiative (IDDSI) test	724:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	6	49	dep	RESULTS	980:986	arg1	Products					988:995	Products B, C, and E	988:1007	RESULTS Products B, C, and E	980:1007	RESULTS Products B, C, and E presented similar composition.					
31812968	6	49	dep	RESULTS	980:986	arg1	C					1000:1000	C	1000:1000	C	1000:1000	RESULTS Products B, C, and E presented similar composition.					
31812968	6	49	dep	RESULTS	980:986	arg1	E					1007:1007	E	1007:1007	E	1007:1007	RESULTS Products B, C, and E presented similar composition.					
31812968	6	49	dep	RESULTS	980:986	arg1	B					997:997	B	997:997	B	997:997	RESULTS Products B, C, and E presented similar composition.					
31812968	10	50	theme	Dysphagia	1482:1490	arg1	Diet					1492:1495	National Dysphagia Diet	1473:1495	National Dysphagia Diet	1473:1495	There was no established correlation between viscosity classifications provided by National Dysphagia Diet and IDDSI.					
31812968	2	51	theme	proper	313:318	arg1	preparation					320:330	the proper preparation	309:330	the proper preparation of modified food	309:347	Commercial thickeners are prescribed for these patients to change food viscosity and may alter the proper preparation of modified food.					
31812968	4	52	theme	available	622:630	arg1	brands					615:620	different brands	605:620	different brands available in Brazil	605:640	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	6	53	theme	similar	1019:1025	arg1	composition					1027:1037	similar composition	1019:1037	similar composition	1019:1037	RESULTS Products B, C, and E presented similar composition.					
31812968	8	54	dep	level	1279:1283	arg1	out					1250:1252	out	1250:1252	out	1250:1252	Viscosity tests revealed that thickener C was basically solid while D displayed results out of the desired viscosity level.					
31812968	9	55	theme	details	1381:1387	arg1	lack					1367:1370	the lack	1363:1370	the lack of label details	1363:1387	CONCLUSIONS The study showed differences in components and viscosity, beyond the lack of label details.					
31812968	4	56	from	Brazil	635:640	arg1	available					622:630	available	622:630	available	622:630	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	4	57	dep	METHODS	556:562	arg1	comprised					575:583	comprised	575:583	comprised all thickeners from different brands available in Brazil, named A to G. Products	575:664	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
31812968	4	58	theme	viscosity	684:692	arg1	analysis					694:701	viscosity analysis	684:701	viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test	684:795	METHODS The sample comprised all thickeners from different brands available in Brazil, named A to G. Products were submitted to viscosity analysis using viscometer and the International Dysphagia Diet Standardization Initiative (IDDSI) test.					
29924315	7	0	theme	-Gal	1303:1306	arg1	terminus					1344:1351	a quantitatively minor terminus	1321:1351	a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan	1321:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	0	theme	-Gal	1303:1306	arg1	determinant					1308:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	10	1	theme	Siglec-8	1680:1687	arg1	ligand					1689:1694	Siglec-8 ligand	1680:1694	Siglec-8 ligand purified from human trachea extracts	1680:1731	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	3	2	theme	sialoglycan	515:525	arg1	ligands					527:533	Siglec-8 sialoglycan ligands	506:533	Siglec-8 sialoglycan ligands on inflammatory target tissues	506:564	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	0	3	theme	Sialylated	0:9	arg1	proteoglycans					27:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	0	3	theme	Sialylated	0:9	arg1	ligands					54:60	Siglec-8 ligands	45:60	Siglec-8 ligands	45:60	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	4	4	theme	human	721:725	arg1	trachea					727:733	postmortem human trachea	710:733	postmortem human trachea	710:733	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	10	5	theme	human	1710:1714	arg1	extracts					1724:1731	human trachea extracts	1710:1731	human trachea extracts	1710:1731	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	2	6	theme	cells-primarily	301:315	arg1	eosinophils					317:327	human allergic inflammatory cells-primarily eosinophils	273:327	human allergic inflammatory cells-primarily eosinophils	273:327	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	2	7	theme	allergic	279:286	arg1	eosinophils					317:327	human allergic inflammatory cells-primarily eosinophils	273:327	human allergic inflammatory cells-primarily eosinophils	273:327	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	4	8	from	airways	677:683	arg1	sialoglycans					653:664	Siglec-8-binding sialoglycans	636:664	Siglec-8-binding sialoglycans from human airways	636:683	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	5	9	theme	ligands	915:921	arg1	classes					895:901	Three size classes	884:901	Three size classes of Siglec-8 ligands	884:921	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	10	10	theme	increased	1741:1749	arg1	apoptosis					1751:1759	increased apoptosis	1741:1759	increased apoptosis of freshly isolated human eosinophils	1741:1797	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	9	11	theme	tracheal	1569:1576	arg1	sections					1591:1598	human tracheal histological sections	1563:1598	human tracheal histological sections	1563:1598	Treating human tracheal histological sections with keratanase also completely eliminated the binding of Siglec-8-Fc.					
29924315	8	12	theme	Siglec-8-binding	1523:1538	arg1	chains					1513:1518	sialylated KS chains	1499:1518	sialylated KS chains	1499:1518	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	8	12	theme	Siglec-8-binding	1523:1538	arg1	determinants					1540:1551	Siglec-8-binding determinants	1523:1551	Siglec-8-binding determinants	1523:1551	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	4	13	theme	composite	786:794	arg1	electrophoresis					819:833	composite agarose-acrylamide gel electrophoresis	786:833	composite agarose-acrylamide gel electrophoresis	786:833	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	1	14	theme	acid-binding	120:131	arg1	proteins					133:140	14 sialic acid-binding proteins	110:140	14 sialic acid-binding proteins	110:140	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	6	15	theme	Proteomic	1071:1079	arg1	spectrometry					1086:1097	Proteomic mass spectrometry	1071:1097	Proteomic mass spectrometry	1071:1097	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	11	16	gly	sialylated	1826:1835	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	16	gly	sialylated	1826:1835	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	17	theme	human	1869:1873	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	17	theme	human	1869:1873	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	0	18	theme	human	65:69	arg1	airways					71:77	human airways	65:77	human airways	65:77	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	7	19	theme	terminal	1275:1282	arg1	terminus					1344:1351	a quantitatively minor terminus	1321:1351	a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan	1321:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	19	theme	terminal	1275:1282	arg1	determinant					1308:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	0	20	from	ligands	54:60	arg1	airways					71:77	human airways	65:77	human airways	65:77	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	8	21	theme	tracheal	1412:1419	arg1	extracts					1421:1428	human tracheal extracts	1406:1428	human tracheal extracts	1406:1428	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	10	22	theme	human	1781:1785	arg1	eosinophils					1787:1797	freshly isolated human eosinophils	1764:1797	freshly isolated human eosinophils	1764:1797	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	1	23	theme	Human	80:84	arg1	siglecs					86:92	Human siglecs	80:92	Human siglecs	80:92	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	1	23	theme	Human	80:84	arg1	family					100:105	a family	98:105	a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses	98:233	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	7	24	theme	aggrecan	1387:1394	arg1	chains					1377:1382	keratan sulfate (KS) chains	1356:1382	keratan sulfate (KS) chains of aggrecan	1356:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	4	25	theme	gel	815:817	arg1	electrophoresis					819:833	composite agarose-acrylamide gel electrophoresis	786:833	composite agarose-acrylamide gel electrophoresis	786:833	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	2	26	theme	mast	402:405	arg1	release					421:427	mast cell mediator release	402:427	mast cell mediator release	402:427	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	5	27	theme	size	890:893	arg1	classes					895:901	Three size classes	884:901	Three size classes of Siglec-8 ligands	884:921	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	11	28	theme	sialylated	1826:1835	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	28	theme	sialylated	1826:1835	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	0	29	theme	sulfate	19:25	arg1	proteoglycans					27:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	0	29	theme	sulfate	19:25	arg1	ligands					54:60	Siglec-8 ligands	45:60	Siglec-8 ligands	45:60	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	7	30	theme	synthetic	1250:1258	arg1	glycans					1260:1266	synthetic glycans	1250:1266	synthetic glycans	1250:1266	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	3	31	theme	human	465:469	arg1	eosinophils					471:481	human eosinophils	465:481	human eosinophils	465:481	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	7	32	theme	sulfate	1364:1370	arg1	chains					1377:1382	keratan sulfate (KS) chains	1356:1382	keratan sulfate (KS) chains of aggrecan	1356:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	33	theme	array	1203:1207	arg1	studies					1209:1215	Glycan array studies	1196:1215	Glycan array studies	1196:1215	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	11	34	theme	endogenous	1858:1867	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	34	theme	endogenous	1858:1867	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	4	35	theme	blot	870:873	arg1	overlay					875:881	Siglec-8-Fc blot overlay	858:881	Siglec-8-Fc blot overlay	858:881	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	2	36	theme	mast	333:336	arg1	engagement					350:359	mast cells-where engagement	333:359	mast cells-where engagement	333:359	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	5	37	theme	pentameric	1042:1051	arg1	form					1053:1056	a recombinant pentameric form	1028:1056	a recombinant pentameric form of Siglec-8	1028:1068	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	7	38	theme	minor	1338:1342	arg1	terminus					1344:1351	a quantitatively minor terminus	1321:1351	a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan	1321:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	38	theme	minor	1338:1342	arg1	determinant					1308:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	8	39	theme	KS	1510:1511	arg1	chains					1513:1518	sialylated KS chains	1499:1518	sialylated KS chains	1499:1518	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	8	39	theme	KS	1510:1511	arg1	determinants					1540:1551	Siglec-8-binding determinants	1523:1551	Siglec-8-binding determinants	1523:1551	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	2	40	theme	eosinophil	368:377	arg1	apoptosis					379:387	eosinophil apoptosis	368:387	eosinophil apoptosis	368:387	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	3	41	from	ligands	527:533	arg1	tissues					558:564	inflammatory target tissues	538:564	inflammatory target tissues	538:564	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	2	42	theme	human	273:277	arg1	eosinophils					317:327	human allergic inflammatory cells-primarily eosinophils	273:327	human allergic inflammatory cells-primarily eosinophils	273:327	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	8	43	gly	sialylated	1499:1508	arg1	chains					1513:1518	sialylated KS chains	1499:1518	sialylated KS chains	1499:1518	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	8	43	gly	sialylated	1499:1508	arg1	determinants					1540:1551	Siglec-8-binding determinants	1523:1551	Siglec-8-binding determinants	1523:1551	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	3	44	theme	target	551:556	arg1	tissues					558:564	inflammatory target tissues	538:564	inflammatory target tissues	538:564	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	10	45	theme	trachea	1716:1722	arg1	extracts					1724:1731	human trachea extracts	1710:1731	human trachea extracts	1710:1731	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	7	46	from	terminus	1344:1351	arg1	chains					1377:1382	keratan sulfate (KS) chains	1356:1382	keratan sulfate (KS) chains of aggrecan	1356:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	4	47	theme	postmortem	710:719	arg1	trachea					727:733	postmortem human trachea	710:733	postmortem human trachea	710:733	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	2	48	theme	inflammatory	288:299	arg1	eosinophils					317:327	human allergic inflammatory cells-primarily eosinophils	273:327	human allergic inflammatory cells-primarily eosinophils	273:327	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	5	49	theme	Siglec-8	906:913	arg1	ligands					915:921	Siglec-8 ligands	906:921	Siglec-8 ligands	906:921	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	4	50	theme	human	671:675	arg1	airways					677:683	human airways	671:683	human airways	671:683	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	2	51	theme	cells-where	338:348	arg1	engagement					350:359	mast cells-where engagement	333:359	mast cells-where engagement	333:359	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	9	52	theme	human	1563:1567	arg1	sections					1591:1598	human tracheal histological sections	1563:1598	human tracheal histological sections	1563:1598	Treating human tracheal histological sections with keratanase also completely eliminated the binding of Siglec-8-Fc.					
29924315	3	53	theme	tissue	609:614	arg1	damage					616:621	tissue damage	609:621	tissue damage	609:621	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	9	54	theme	histological	1578:1589	arg1	sections					1591:1598	human tracheal histological sections	1563:1598	human tracheal histological sections	1563:1598	Treating human tracheal histological sections with keratanase also completely eliminated the binding of Siglec-8-Fc.					
29924315	4	55	theme	size-exclusion	752:765	arg1	chromatography					767:780	size-exclusion chromatography	752:780	size-exclusion chromatography	752:780	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	1	56	theme	sialic	113:118	arg1	proteins					133:140	14 sialic acid-binding proteins	110:140	14 sialic acid-binding proteins	110:140	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	3	57	theme	allergic	577:584	arg1	inflammation					586:597	allergic inflammation	577:597	allergic inflammation	577:597	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	6	58	theme	mass	1081:1084	arg1	spectrometry					1086:1097	Proteomic mass spectrometry	1071:1097	Proteomic mass spectrometry	1071:1097	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	7	59	theme	6-sulfo	1295:1301	arg1	terminus					1344:1351	a quantitatively minor terminus	1321:1351	a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan	1321:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	59	theme	6-sulfo	1295:1301	arg1	determinant					1308:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	1	60	theme	proteins	133:140	arg1	siglecs					86:92	Human siglecs	80:92	Human siglecs	80:92	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	1	60	theme	proteins	133:140	arg1	family					100:105	a family	98:105	a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses	98:233	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	11	61	theme	airway	1875:1880	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	61	theme	airway	1875:1880	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	7	62	theme	Neu5Acα2-3	1284:1293	arg1	terminus					1344:1351	a quantitatively minor terminus	1321:1351	a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan	1321:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	62	theme	Neu5Acα2-3	1284:1293	arg1	determinant					1308:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	a terminal Neu5Acα2-3(6-sulfo)-Gal determinant	1273:1318	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	5	63	theme	affinity	998:1005	arg1	chromatography					1007:1020	affinity chromatography	998:1020	affinity chromatography using a recombinant pentameric form of Siglec-8	998:1068	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	9	64	theme	Siglec-8-Fc	1658:1668	arg1	binding					1647:1653	the binding	1643:1653	the binding of Siglec-8-Fc	1643:1668	Treating human tracheal histological sections with keratanase also completely eliminated the binding of Siglec-8-Fc.					
29924315	6	65	theme	proteoglycan	1134:1145	arg1	aggrecan					1147:1154	the proteoglycan aggrecan	1130:1154	the proteoglycan aggrecan	1130:1154	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	6	65	theme	proteoglycan	1134:1145	arg1	finding					1159:1165	a finding	1157:1165	a finding validated by immunoblotting	1157:1193	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	6	65	theme	proteoglycan	1134:1145	arg1	classes					1119:1125	all size classes	1110:1125	all size classes	1110:1125	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	8	66	theme	human	1406:1410	arg1	extracts					1421:1428	human tracheal extracts	1406:1428	human tracheal extracts	1406:1428	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	10	67	theme	isolated	1772:1779	arg1	eosinophils					1787:1797	freshly isolated human eosinophils	1764:1797	freshly isolated human eosinophils	1764:1797	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	0	68	theme	keratan	11:17	arg1	proteoglycans					27:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	0	68	theme	keratan	11:17	arg1	ligands					54:60	Siglec-8 ligands	45:60	Siglec-8 ligands	45:60	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	11	69	theme	allergic	1926:1933	arg1	inflammation					1935:1946	allergic inflammation	1926:1946	allergic inflammation	1926:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	4	70	theme	agarose-acrylamide	796:813	arg1	electrophoresis					819:833	composite agarose-acrylamide gel electrophoresis	786:833	composite agarose-acrylamide gel electrophoresis	786:833	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	7	71	theme	Siglec-8	1230:1237	arg1	binding					1239:1245	Siglec-8 binding	1230:1245	Siglec-8 binding to synthetic glycans	1230:1266	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	4	72	dep	chromatography	767:780	arg1	probed					848:853	probed	848:853	probed by Siglec-8-Fc blot overlay	848:881	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	4	72	dep	chromatography	767:780	arg1	blotted					836:842	blotted	836:842	blotted	836:842	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	6	73	theme	size	1114:1117	arg1	aggrecan					1147:1154	the proteoglycan aggrecan	1130:1154	the proteoglycan aggrecan	1130:1154	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	6	73	theme	size	1114:1117	arg1	classes					1119:1125	all size classes	1110:1125	all size classes	1110:1125	Proteomic mass spectrometry identified all size classes as the proteoglycan aggrecan, a finding validated by immunoblotting.					
29924315	3	74	theme	mast	487:490	arg1	cells					492:496	mast cells	487:496	mast cells	487:496	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	0	75	theme	Siglec-8	45:52	arg1	proteoglycans					27:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans	0:39	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	0	75	theme	Siglec-8	45:52	arg1	ligands					54:60	Siglec-8 ligands	45:60	Siglec-8 ligands	45:60	Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways.					
29924315	1	76	theme	immune	185:190	arg1	cells					192:196	immune cells	185:196	immune cells where they regulate immune responses	185:233	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	5	77	theme	Siglec-8	1061:1068	arg1	form					1053:1056	a recombinant pentameric form	1028:1056	a recombinant pentameric form of Siglec-8	1028:1068	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	7	78	theme	keratan	1356:1362	arg1	KS					1373:1374	KS	1373:1374	KS	1373:1374	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	7	78	theme	keratan	1356:1362	arg1	sulfate					1364:1370	keratan sulfate	1356:1370	keratan sulfate (KS) chains of aggrecan	1356:1394	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	2	79	theme	mediator	412:419	arg1	release					421:427	mast cell mediator release	402:427	mast cell mediator release	402:427	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	10	80	theme	eosinophils	1787:1797	arg1	apoptosis					1751:1759	increased apoptosis	1741:1759	increased apoptosis of freshly isolated human eosinophils	1741:1797	Finally, Siglec-8 ligand purified from human trachea extracts induced increased apoptosis of freshly isolated human eosinophils in vitro.					
29924315	7	81	theme	Glycan	1196:1201	arg1	studies					1209:1215	Glycan array studies	1196:1215	Glycan array studies	1196:1215	Glycan array studies demonstrated Siglec-8 binding to synthetic glycans with a terminal Neu5Acα2-3(6-sulfo)-Gal determinant, a quantitatively minor terminus on keratan sulfate (KS) chains of aggrecan.					
29924315	8	82	theme	Siglec-8	1470:1477	arg1	binding					1479:1485	Siglec-8 binding	1470:1485	Siglec-8 binding	1470:1485	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	3	83	theme	Siglec-8	506:513	arg1	ligands					527:533	Siglec-8 sialoglycan ligands	506:533	Siglec-8 sialoglycan ligands on inflammatory target tissues	506:564	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	5	84	theme	recombinant	1030:1040	arg1	form					1053:1056	a recombinant pentameric form	1028:1056	a recombinant pentameric form of Siglec-8	1028:1068	Three size classes of Siglec-8 ligands were identified: 250 kDa, 600 kDa and 1 MDa, each of which was purified by affinity chromatography using a recombinant pentameric form of Siglec-8.					
29924315	4	85	theme	Siglec-8-binding	636:651	arg1	sialoglycans					653:664	Siglec-8-binding sialoglycans	636:664	Siglec-8-binding sialoglycans from human airways	636:683	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	11	86	theme	KS	1837:1838	arg1	ligands					1882:1888	endogenous human airway ligands	1858:1888	endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation	1858:1946	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	11	86	theme	KS	1837:1838	arg1	proteoglycans					1840:1852	sialylated KS proteoglycans	1826:1852	sialylated KS proteoglycans	1826:1852	We conclude that sialylated KS proteoglycans are endogenous human airway ligands that bind Siglec-8 and may regulate allergic inflammation.					
29924315	4	87	theme	Siglec-8-Fc	858:868	arg1	overlay					875:881	Siglec-8-Fc blot overlay	858:881	Siglec-8-Fc blot overlay	858:881	To identify Siglec-8-binding sialoglycans from human airways, proteins extracted from postmortem human trachea were resolved by size-exclusion chromatography and composite agarose-acrylamide gel electrophoresis, blotted and probed by Siglec-8-Fc blot overlay.					
29924315	1	88	theme	cells	192:196	arg1	subsets					174:180	subsets	174:180	subsets of immune cells where they regulate immune responses	174:233	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29924315	2	89	theme	cell	407:410	arg1	release					421:427	mast cell mediator release	402:427	mast cell mediator release	402:427	Siglec-8 is expressed selectively on human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis and inhibits mast cell mediator release.					
29924315	8	90	theme	sialylated	1499:1508	arg1	chains					1513:1518	sialylated KS chains	1499:1518	sialylated KS chains	1499:1518	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	8	90	theme	sialylated	1499:1508	arg1	determinants					1540:1551	Siglec-8-binding determinants	1523:1551	Siglec-8-binding determinants	1523:1551	Treating human tracheal extracts with sialidase or keratanase eliminated Siglec-8 binding, indicating sialylated KS chains as Siglec-8-binding determinants.					
29924315	3	91	theme	inflammatory	538:549	arg1	tissues					558:564	inflammatory target tissues	538:564	inflammatory target tissues	538:564	Evidence supports a model in which human eosinophils and mast cells bind to Siglec-8 sialoglycan ligands on inflammatory target tissues to resolve allergic inflammation and limit tissue damage.					
29924315	1	92	theme	immune	218:223	arg1	responses					225:233	immune responses	218:233	immune responses	218:233	Human siglecs are a family of 14 sialic acid-binding proteins, most of which are expressed on subsets of immune cells where they regulate immune responses.					
29962278	0	0	theme	hydroxyapatite	81:94	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	4	1	theme	higher	710:715	arg1	stability					717:725	higher stability	710:725	higher stability	710:725	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	7	2	theme	body	1004:1007	arg1	fluid					1009:1013	simulated body fluid	994:1013	simulated body fluid	994:1013	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	9	3	theme	viability	1172:1180	arg1	studies					1182:1188	In vitro cell viability studies	1158:1188	In vitro cell viability studies using mouse osteoblast MC3T3 cells	1158:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	0	4	theme	-nano	75:79	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	10	5	theme	cytotoxicity	1434:1445	arg1	signs					1425:1429	any signs	1421:1429	any signs of cytotoxicity compared to pristine PVA	1421:1470	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	9	6	theme	ethidium	1275:1282	arg1	staining					1308:1315	ethidium bromide/acridine orange staining	1275:1315	ethidium bromide/acridine orange staining	1275:1315	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	7	7	dep	In	948:949	arg1	vitro					951:955	vitro	951:955	vitro	951:955	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	0	8	theme	hydrogels	106:114	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	0	8	theme	hydrogels	106:114	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	11	9	theme	hydrogel	1601:1608	arg1	matrices					1610:1617	composite hydrogel matrices	1591:1617	composite hydrogel matrices	1591:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	9	10	theme	MC3T3	1213:1217	arg1	cells					1219:1223	mouse osteoblast MC3T3 cells	1196:1223	mouse osteoblast MC3T3 cells	1196:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	0	11	theme	composite	96:104	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	10	12	theme	cell	1393:1396	arg1	growth					1398:1403	cell growth	1393:1403	cell growth	1393:1403	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	7	13	theme	biomineralization	957:973	arg1	study					975:979	In vitro biomineralization study	948:979	In vitro biomineralization study performed in simulated body fluid	948:1013	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	7	14	dep	HPG/PVA/n-HA	1021:1032	arg1	has					1034:1036	has	1034:1036	has an apatite like structure	1034:1062	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	11	15	theme	Osteoblastic	1473:1484	arg1	activity					1486:1493	Osteoblastic activity	1473:1493	Osteoblastic activity	1473:1493	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	0	16	theme	bone	120:123	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	10	17	theme	viability	1335:1343	arg1	studies					1345:1351	The cell viability studies	1326:1351	The cell viability studies	1326:1351	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	5	18	theme	tissue	846:851	arg1	regeneration					853:864	bone tissue regeneration	841:864	bone tissue regeneration	841:864	The HPG/PVA hydrogel shows porous morphology as revealed by the SEM, which is suitable for bone tissue regeneration.					
29962278	4	19	theme	thermal	643:649	arg1	stability					651:659	higher thermal stability	636:659	higher thermal stability	636:659	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	2	20	theme	bionanocomposite	302:317	arg1	hydrogels					319:327	novel and ecofriendly bionanocomposite hydrogels	280:327	novel and ecofriendly bionanocomposite hydrogels	280:327	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	10	21	theme	cell	1330:1333	arg1	studies					1345:1351	The cell viability studies	1326:1351	The cell viability studies	1326:1351	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	1	22	theme	natural	185:191	arg1	materials					193:201	natural materials	185:201	natural materials	185:201	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	2	23	theme	reaction	473:480	arg1	conditions					482:491	freeze-thaw and mild reaction conditions	452:491	conditions	482:491	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	7	24	theme	In	948:949	arg1	study					975:979	In vitro biomineralization study	948:979	In vitro biomineralization study performed in simulated body fluid	948:1013	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	2	25	theme	ecofriendly	290:300	arg1	hydrogels					319:327	novel and ecofriendly bionanocomposite hydrogels	280:327	novel and ecofriendly bionanocomposite hydrogels	280:327	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	10	26	theme	pristine	1459:1466	arg1	PVA					1468:1470	pristine PVA	1459:1470	pristine PVA	1459:1470	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	3	27	theme	various	542:548	arg1	techniques					550:559	various techniques	542:559	various techniques	542:559	The hydrogel materials were characterized using various techniques.					
29962278	2	28	theme	alcohol	400:406	arg1	blend					354:358	a blend	352:358	a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA)	352:444	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	8	29	theme	biocompatibility	1132:1147	arg1	studies					1149:1155	biocompatibility studies	1132:1155	biocompatibility studies	1132:1155	The hydrogel materials were employed as extracellular matrices for biocompatibility studies.					
29962278	9	30	theme	Trypan	1248:1253	arg1	exclusion					1260:1268	Trypan blue exclusion	1248:1268	Trypan blue exclusion	1248:1268	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	10	31	theme	composite	1365:1373	arg1	materials					1375:1383	composite materials	1365:1383	composite materials	1365:1383	The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA.					
29962278	2	32	theme	vinyl	394:398	arg1	PVA					409:411	PVA	409:411	PVA	409:411	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	2	32	theme	vinyl	394:398	arg1	alcohol					400:406	poly vinyl alcohol	389:406	poly vinyl alcohol (PVA)	389:412	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	8	33	theme	extracellular	1105:1117	arg1	matrices					1119:1126	extracellular matrices	1105:1126	extracellular matrices for biocompatibility studies	1105:1155	The hydrogel materials were employed as extracellular matrices for biocompatibility studies.					
29962278	8	33	theme	extracellular	1105:1117	arg1	materials					1078:1086	The hydrogel materials	1065:1086	The hydrogel materials	1065:1086	The hydrogel materials were employed as extracellular matrices for biocompatibility studies.					
29962278	9	34	theme	blue	1255:1258	arg1	exclusion					1260:1268	Trypan blue exclusion	1248:1268	Trypan blue exclusion	1248:1268	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	11	35	theme	phosphatase	1534:1544	arg1	activity					1553:1560	an increased alkaline phosphatase enzyme activity	1512:1560	an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices	1512:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	11	36	theme	increased	1515:1523	arg1	activity					1553:1560	an increased alkaline phosphatase enzyme activity	1512:1560	an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices	1512:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	2	37	theme	nano-hydroxyapatite	419:437	arg1	blend					354:358	a blend	352:358	a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA)	352:444	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	9	38	theme	mouse	1196:1200	arg1	cells					1219:1223	mouse osteoblast MC3T3 cells	1196:1223	mouse osteoblast MC3T3 cells	1196:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	0	39	theme	tissue	125:130	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	9	40	theme	exclusion	1260:1268	arg1	methods					1317:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	11	41	from	activity	1553:1560	arg1	cells					1576:1580	MC3T3 bone cells	1565:1580	MC3T3 bone cells grown on composite hydrogel matrices	1565:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	1	42	theme	bone	159:162	arg1	implants					164:171	Biocompatible bone implants	145:171	Biocompatible bone implants composed of natural materials	145:201	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	0	43	theme	guar-poly	50:58	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	3	44	theme	hydrogel	498:505	arg1	materials					507:515	The hydrogel materials	494:515	The hydrogel materials	494:515	The hydrogel materials were characterized using various techniques.					
29962278	11	45	theme	composite	1591:1599	arg1	matrices					1610:1617	composite hydrogel matrices	1591:1617	composite hydrogel matrices	1591:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	9	46	theme	In	1158:1159	arg1	studies					1182:1188	In vitro cell viability studies	1158:1188	In vitro cell viability studies using mouse osteoblast MC3T3 cells	1158:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	1	47	theme	orthopedic	227:236	arg1	procedures					253:262	orthopedic reconstruction procedures	227:262	orthopedic reconstruction procedures	227:262	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	2	48	theme	mild	468:471	arg1	conditions					482:491	freeze-thaw and mild reaction conditions	452:491	conditions	482:491	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	0	49	theme	hydroxypropyl	36:48	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	1	50	theme	reconstruction	238:251	arg1	procedures					253:262	orthopedic reconstruction procedures	227:262	orthopedic reconstruction procedures	227:262	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	0	51	theme	vinyl	61:65	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	6	52	from	transparent	912:922	arg1	nature					940:945	nature	940:945	nature	940:945	Additionally, the hydrogels were found to be transparent and flexible in nature.					
29962278	9	53	dep	In	1158:1159	arg1	vitro					1161:1165	vitro	1161:1165	vitro	1161:1165	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	9	54	theme	cell	1167:1170	arg1	studies					1182:1188	In vitro cell viability studies	1158:1188	In vitro cell viability studies using mouse osteoblast MC3T3 cells	1158:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	2	55	theme	freeze-thaw	452:462	arg1	conditions					482:491	freeze-thaw and mild reaction conditions	452:491	conditions	482:491	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	4	56	theme	TGA	562:564	arg1	studies					566:572	TGA studies	562:572	TGA studies	562:572	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	6	57	from	nature	940:945	arg1	transparent					912:922	transparent	912:922	transparent	912:922	Additionally, the hydrogels were found to be transparent and flexible in nature.					
29962278	6	57	from	nature	940:945	arg1	hydrogels					885:893	the hydrogels	881:893	the hydrogels	881:893	Additionally, the hydrogels were found to be transparent and flexible in nature.					
29962278	9	58	theme	bromide/acridine	1284:1299	arg1	staining					1308:1315	ethidium bromide/acridine orange staining	1275:1315	ethidium bromide/acridine orange staining	1275:1315	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	8	59	theme	hydrogel	1069:1076	arg1	matrices					1119:1126	extracellular matrices	1105:1126	extracellular matrices for biocompatibility studies	1105:1155	The hydrogel materials were employed as extracellular matrices for biocompatibility studies.					
29962278	8	59	theme	hydrogel	1069:1076	arg1	materials					1078:1086	The hydrogel materials	1065:1086	The hydrogel materials	1065:1086	The hydrogel materials were employed as extracellular matrices for biocompatibility studies.					
29962278	1	60	from	desirable	214:222	arg1	procedures					253:262	orthopedic reconstruction procedures	227:262	orthopedic reconstruction procedures	227:262	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	2	61	theme	guar	377:380	arg1	blend					354:358	a blend	352:358	a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA)	352:444	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	11	62	theme	enzyme	1546:1551	arg1	activity					1553:1560	an increased alkaline phosphatase enzyme activity	1512:1560	an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices	1512:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	1	63	from	procedures	253:262	arg1	desirable					214:222	desirable	214:222	desirable	214:222	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	9	64	theme	MTT	1243:1245	arg1	methods					1317:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	4	65	contain	have	631:634	arg1	HPG/PVA					605:611	HPG/PVA	605:611	HPG/PVA	605:611	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	4	65	contain	have	631:634	arg1	composites					593:602	both composites	588:602	both composites	588:602	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	4	65	contain	have	631:634	arg1	HPG/PVA/n-HA					617:628	HPG/PVA/n-HA	617:628	HPG/PVA/n-HA	617:628	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	4	65	contain	have	631:634	arg2	stability					651:659	higher thermal stability	636:659	higher thermal stability	636:659	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	2	66	theme	hydroxypropyl	363:375	arg1	guar					377:380	hydroxypropyl guar	363:380	hydroxypropyl guar (HPG)	363:386	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	2	66	theme	hydroxypropyl	363:375	arg1	HPG					383:385	HPG	383:385	HPG	383:385	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	9	67	theme	osteoblast	1202:1211	arg1	cells					1219:1223	mouse osteoblast MC3T3 cells	1196:1223	mouse osteoblast MC3T3 cells	1196:1223	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	9	68	theme	staining	1308:1315	arg1	methods					1317:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods	1243:1323	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	2	69	theme	novel	280:284	arg1	hydrogels					319:327	novel and ecofriendly bionanocomposite hydrogels	280:327	novel and ecofriendly bionanocomposite hydrogels	280:327	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	1	70	theme	Biocompatible	145:157	arg1	implants					164:171	Biocompatible bone implants	145:171	Biocompatible bone implants composed of natural materials	145:201	Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures.					
29962278	11	71	theme	alkaline	1525:1532	arg1	activity					1553:1560	an increased alkaline phosphatase enzyme activity	1512:1560	an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices	1512:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	5	72	theme	porous	777:782	arg1	morphology					784:793	porous morphology	777:793	porous morphology	777:793	The HPG/PVA hydrogel shows porous morphology as revealed by the SEM, which is suitable for bone tissue regeneration.					
29962278	11	73	theme	MC3T3	1565:1569	arg1	cells					1576:1580	MC3T3 bone cells	1565:1580	MC3T3 bone cells grown on composite hydrogel matrices	1565:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	5	74	theme	bone	841:844	arg1	regeneration					853:864	bone tissue regeneration	841:864	bone tissue regeneration	841:864	The HPG/PVA hydrogel shows porous morphology as revealed by the SEM, which is suitable for bone tissue regeneration.					
29962278	2	75	theme	poly	389:392	arg1	PVA					409:411	PVA	409:411	PVA	409:411	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	2	75	theme	poly	389:392	arg1	alcohol					400:406	poly vinyl alcohol	389:406	poly vinyl alcohol (PVA)	389:412	In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions.					
29962278	9	76	theme	orange	1301:1306	arg1	staining					1308:1315	ethidium bromide/acridine orange staining	1275:1315	ethidium bromide/acridine orange staining	1275:1315	In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods.					
29962278	4	77	theme	higher	636:641	arg1	stability					651:659	higher thermal stability	636:659	higher thermal stability	636:659	TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone.					
29962278	0	78	theme	alcohol	67:73	arg1	hydrogels					106:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels	36:114	hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering	36:142	Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering.					
29962278	7	79	theme	simulated	994:1002	arg1	fluid					1009:1013	simulated body fluid	994:1013	simulated body fluid	994:1013	In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure.					
29962278	11	80	theme	bone	1571:1574	arg1	cells					1576:1580	MC3T3 bone cells	1565:1580	MC3T3 bone cells grown on composite hydrogel matrices	1565:1617	Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.					
29962278	5	81	theme	HPG/PVA	754:760	arg1	hydrogel					762:769	The HPG/PVA hydrogel	750:769	The HPG/PVA hydrogel	750:769	The HPG/PVA hydrogel shows porous morphology as revealed by the SEM, which is suitable for bone tissue regeneration.					
29890316	7	0	theme	fecal	1223:1227	arg1	samples					1229:1235	human fecal samples	1217:1235	human fecal samples	1217:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	3	1	theme	effective	485:493	arg1	component					495:503	the major effective component	475:503	the major effective component	475:503	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	12	2	theme	acid	2006:2009	arg1	contents					1987:1994	increased contents	1977:1994	increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus	1977:2141	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	3	3	from	component	495:503	arg1	SJZD					508:511	SJZD	508:511	SJZD	508:511	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	13	4	theme	immunomodulatory	2170:2185	arg1	role					2187:2190	an immunomodulatory role	2167:2190	an immunomodulatory role	2167:2190	CONCLUSION S-3-1 plays an immunomodulatory role by regulating the abundances of 9 intestinal bacteria genera.					
29890316	12	5	theme	total	2015:2019	arg1	acid					2021:2024	total acid	2015:2024	total acid	2015:2024	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	7	6	theme	Gas	1119:1121	arg1	GC					1138:1139	GC	1138:1139	GC	1138:1139	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	7	6	theme	Gas	1119:1121	arg1	chromatograph					1123:1135	Gas chromatograph	1119:1135	Gas chromatograph (GC) analysis	1119:1149	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	5	7	theme	intestinal	851:860	arg1	juice					862:866	intestinal juice	851:866	intestinal juice	851:866	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	12	8	theme	increased	1977:1985	arg1	contents					1987:1994	increased contents	1977:1994	increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus	1977:2141	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	10	9	theme	Dorea	1591:1595	arg1	abundances					1491:1500	the abundances	1487:1500	the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea	1487:1595	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	14	10	theme	SCFA	2345:2348	arg1	content					2350:2356	the SCFA content	2341:2356	the SCFA content to affect immunomodulation	2341:2383	Incubated S-3-1 can regulate more bacteria genera, a total of 13 kinds, and can adjust the SCFA content to affect immunomodulation.					
29890316	6	11	theme	reducing	975:982	arg1	sugar					984:988	reducing sugar	975:988	reducing sugar	975:988	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	8	12	theme	gut	1248:1250	arg1	composition					1263:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	5	13	theme	simulated	826:834	arg1	juice					844:848	simulated gastric juice	826:848	simulated gastric juice	826:848	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	6	14	theme	polysaccharide	956:969	arg1	concentrations					932:945	The concentrations	928:945	The concentrations of total polysaccharide and reducing sugar	928:988	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	4	15	theme	homogeneous	612:622	arg1	S-3-1					603:607	S-3-1	603:607	S-3-1	603:607	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	4	15	theme	homogeneous	612:622	arg1	polysaccharide					624:637	a homogeneous polysaccharide	610:637	a homogeneous polysaccharide purified from SJZD with immune-enhancement activity	610:689	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	10	16	theme	microflora	1617:1626	arg1	samples					1628:1634	human intestinal microflora samples	1600:1634	human intestinal microflora samples	1600:1634	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	4	17	dep	aimed	549:553	arg1	AIM					514:516	AIM	514:516	AIM OF THE STUDY	514:529	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	1	18	from	recipe	199:204	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine (TCM)	209:242	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	18	from	recipe	199:204	arg1	TCM					239:241	TCM	239:241	TCM	239:241	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	16	19	theme	pharmacological	2619:2633	arg1	mechanisms					2635:2644	the pharmacological mechanisms	2615:2644	the pharmacological mechanisms of polysaccharides	2615:2663	This study could provide new insights for studies on the pharmacological mechanisms of polysaccharides in vitro.					
29890316	10	20	theme	human	1600:1604	arg1	samples					1628:1634	human intestinal microflora samples	1600:1634	human intestinal microflora samples	1600:1634	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	15	21	theme	intestinal	2478:2487	arg1	flora					2489:2493	the intestinal flora	2474:2493	the intestinal flora composition	2474:2505	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	16	22	from	studies	2604:2610	arg1	mechanisms					2635:2644	the pharmacological mechanisms	2615:2644	the pharmacological mechanisms of polysaccharides	2615:2663	This study could provide new insights for studies on the pharmacological mechanisms of polysaccharides in vitro.					
29890316	7	23	from	contents	1205:1212	arg1	samples					1229:1235	human fecal samples	1217:1235	human fecal samples	1217:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	12	24	theme	Enterococcus	2085:2096	arg1	abundances					2071:2080	the abundances	2067:2080	the abundances of Enterococcus	2067:2096	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	24	theme	Enterococcus	2085:2096	arg1	Butyricimonas					2111:2123	Butyricimonas	2111:2123	Butyricimonas	2111:2123	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	24	theme	Enterococcus	2085:2096	arg1	Sutterella					2099:2108	Sutterella	2099:2108	Sutterella	2099:2108	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	24	theme	Enterococcus	2085:2096	arg1	Streptococcus					2129:2141	Streptococcus	2129:2141	Streptococcus	2129:2141	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	4	25	theme	immune-enhancement	663:680	arg1	activity					682:689	immune-enhancement activity	663:689	immune-enhancement activity	663:689	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	11	26	theme	influenced	1738:1747	arg1	flora					1749:1753	the influenced flora	1734:1753	the influenced flora	1734:1753	Specifically, S-3-1 could only regulate the abundances of Paraprevotella and Oscillospira, while the influenced flora changed to Butyricimonas, Coprococcus, Dialister, Sutterella, Ruminococcus and Parabacteroides after sequential incubation of S-3-1.					
29890316	7	27	theme	human	1217:1221	arg1	samples					1229:1235	human fecal samples	1217:1235	human fecal samples	1217:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	5	28	theme	human	873:877	arg1	microflora					885:894	human fecal microflora	873:894	human fecal microflora	873:894	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	6	29	from	stability	1020:1028	arg1	models					1111:1116	three in vitro fermentation models	1083:1116	three in vitro fermentation models	1083:1116	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	8	30	theme	MiSeq	1314:1318	arg1	sequencing					1320:1329	rRNA gene Illumina MiSeq sequencing	1295:1329	rRNA gene Illumina MiSeq sequencing (V3-V4 region)	1295:1344	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	8	30	theme	MiSeq	1314:1318	arg1	region					1338:1343	V3-V4 region	1332:1343	V3-V4 region	1332:1343	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	0	31	theme	intestinal	72:81	arg1	microbes					83:90	human intestinal microbes	66:90	human intestinal microbes	66:90	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	11	32	theme	Paraprevotella	1695:1708	arg1	abundances					1681:1690	the abundances	1677:1690	the abundances of Paraprevotella and Oscillospira	1677:1725	Specifically, S-3-1 could only regulate the abundances of Paraprevotella and Oscillospira, while the influenced flora changed to Butyricimonas, Coprococcus, Dialister, Sutterella, Ruminococcus and Parabacteroides after sequential incubation of S-3-1.					
29890316	0	33	from	Effects	0:6	arg1	Sijunzi					45:51	Sijunzi	45:51	Sijunzi	45:51	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	4	34	theme	short	754:758	arg1	acids					772:776	short chain fatty acids	754:776	short chain fatty acids	754:776	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	1	35	dep	RELEVANCE	151:159	arg1	SJZD					180:183	SJZD	180:183	SJZD	180:183	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	35	dep	RELEVANCE	151:159	arg1	decoction					169:177	Sijunzi decoction	161:177	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	16	36	theme	new	2587:2589	arg1	insights					2591:2598	new insights	2587:2598	new insights	2587:2598	This study could provide new insights for studies on the pharmacological mechanisms of polysaccharides in vitro.					
29890316	0	37	from	Sijunzi	45:51	arg1	Effects					0:6	Effects	0:6	Effects of a homogeneous polysaccharide from Sijunzi	0:51	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	0	37	from	Sijunzi	45:51	arg1	polysaccharide					25:38	a homogeneous polysaccharide	11:38	a homogeneous polysaccharide from Sijunzi	11:51	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	10	38	theme	incubated	1456:1464	arg1	S-3-1					1466:1470	incubated S-3-1	1456:1470	incubated S-3-1	1456:1470	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	4	39	theme	fatty	766:770	arg1	acids					772:776	short chain fatty acids	754:776	short chain fatty acids	754:776	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	0	40	theme	chain	102:106	arg1	acids					114:118	short chain fatty acids	96:118	short chain fatty acids	96:118	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	6	41	theme	in	1089:1090	arg1	models					1111:1116	three in vitro fermentation models	1083:1116	three in vitro fermentation models	1083:1116	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	4	42	theme	human	724:728	arg1	microbes					741:748	human intestinal microbes	724:748	human intestinal microbes	724:748	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	7	43	theme	chain	1181:1185	arg1	SCFA					1199:1202	SCFA	1199:1202	SCFA	1199:1202	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	7	43	theme	chain	1181:1185	arg1	acid					1193:1196	the short chain fatty acid	1171:1196	the short chain fatty acid (SCFA) contents in human fecal samples	1171:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	13	44	theme	bacteria	2237:2244	arg1	genera					2246:2251	9 intestinal bacteria genera	2224:2251	9 intestinal bacteria genera	2224:2251	CONCLUSION S-3-1 plays an immunomodulatory role by regulating the abundances of 9 intestinal bacteria genera.					
29890316	2	45	theme	loose	409:413	arg1	stools					415:420	loose stools	409:420	loose stools	409:420	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	6	46	theme	incubated	1064:1072	arg1	S-3-1					1074:1078	independently and sequentially incubated S-3-1	1033:1078	independently and sequentially incubated S-3-1	1033:1078	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	15	47	with	Incubation	2386:2395	arg1	juices					2425:2430	gastric and intestinal juices	2402:2430	gastric and intestinal juices	2402:2430	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	2	48	theme	reduced	385:391	arg1	intake					398:403	reduced food intake	385:403	reduced food intake	385:403	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	0	49	theme	polysaccharide	25:38	arg1	Effects					0:6	Effects	0:6	Effects of a homogeneous polysaccharide from Sijunzi	0:51	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	9	50	theme	RESULTS	1347:1353	arg1	S-3-1					1355:1359	RESULTS S-3-1	1347:1359	RESULTS S-3-1	1347:1359	RESULTS S-3-1 was degraded in three in vitro fermentation models separately and sequentially.					
29890316	3	51	theme	abundant	450:457	arg1	constituent					459:469	the most abundant constituent	441:469	the most abundant constituent	441:469	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	3	51	theme	abundant	450:457	arg1	Polysaccharide					423:436	Polysaccharide	423:436	Polysaccharide	423:436	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	1	52	theme	traditional	209:219	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine (TCM)	209:242	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	52	theme	traditional	209:219	arg1	TCM					239:241	TCM	239:241	TCM	239:241	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	7	53	used	used	1155:1158	arg2	analysis					1142:1149	Gas chromatograph (GC) analysis	1119:1149	Gas chromatograph (GC) analysis	1119:1149	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	8	54	theme	gene	1300:1303	arg1	sequencing					1320:1329	rRNA gene Illumina MiSeq sequencing	1295:1329	rRNA gene Illumina MiSeq sequencing (V3-V4 region)	1295:1344	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	8	54	theme	gene	1300:1303	arg1	region					1338:1343	V3-V4 region	1332:1343	V3-V4 region	1332:1343	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	14	55	theme	Incubated	2254:2262	arg1	S-3-1					2264:2268	Incubated S-3-1	2254:2268	Incubated S-3-1	2254:2268	Incubated S-3-1 can regulate more bacteria genera, a total of 13 kinds, and can adjust the SCFA content to affect immunomodulation.					
29890316	2	56	theme	function	347:354	arg1	disorders					317:325	disorders	317:325	disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools	317:420	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	12	57	theme	acid	2021:2024	arg1	contents					1987:1994	increased contents	1977:1994	increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus	1977:2141	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	7	58	theme	fatty	1187:1191	arg1	SCFA					1199:1202	SCFA	1199:1202	SCFA	1199:1202	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	7	58	theme	fatty	1187:1191	arg1	acid					1193:1196	the short chain fatty acid	1171:1196	the short chain fatty acid (SCFA) contents in human fecal samples	1171:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	3	59	theme	major	479:483	arg1	component					495:503	the major effective component	475:503	the major effective component	475:503	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	9	60	theme	in	1383:1384	arg1	models					1405:1410	three in vitro fermentation models	1377:1410	three in vitro fermentation models	1377:1410	RESULTS S-3-1 was degraded in three in vitro fermentation models separately and sequentially.					
29890316	6	61	theme	fermentation	1098:1109	arg1	models					1111:1116	three in vitro fermentation models	1083:1116	three in vitro fermentation models	1083:1116	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	1	62	theme	classic	191:197	arg1	RELEVANCE					151:159	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	62	theme	classic	191:197	arg1	recipe					199:204	a classic recipe	189:204	a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi	189:284	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	7	63	theme	chromatograph	1123:1135	arg1	analysis					1142:1149	Gas chromatograph (GC) analysis	1119:1149	Gas chromatograph (GC) analysis	1119:1149	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	9	64	theme	fermentation	1392:1403	arg1	models					1405:1410	three in vitro fermentation models	1377:1410	three in vitro fermentation models	1377:1410	RESULTS S-3-1 was degraded in three in vitro fermentation models separately and sequentially.					
29890316	12	65	theme	acetic	1999:2004	arg1	acid					2006:2009	acetic acid	1999:2009	acetic acid	1999:2009	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	5	66	theme	fecal	879:883	arg1	microflora					885:894	human fecal microflora	873:894	human fecal microflora	873:894	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	10	67	dep	Lactobacillus	1505:1517	arg1	Enterococcus					1561:1572	Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus	1505:1572	Enterococcus	1561:1572	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	3	68	from	constituent	459:469	arg1	SJZD					508:511	SJZD	508:511	SJZD	508:511	Polysaccharide is the most abundant constituent and the major effective component in SJZD.					
29890316	6	69	theme	sugar	984:988	arg1	concentrations					932:945	The concentrations	928:945	The concentrations of total polysaccharide and reducing sugar	928:988	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	4	70	theme	S-3-1	603:607	arg1	mechanism					590:598	the immunomodulatory mechanism	569:598	the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity,	569:690	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	5	71	theme	gastric	836:842	arg1	juice					844:848	simulated gastric juice	826:848	simulated gastric juice	826:848	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	12	72	theme	SCFA	1948:1951	arg1	content					1937:1943	the content	1933:1943	the content of SCFA	1933:1951	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	6	73	theme	total	950:954	arg1	polysaccharide					956:969	total polysaccharide	950:969	total polysaccharide	950:969	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	8	74	theme	microbiota	1252:1261	arg1	composition					1263:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	10	75	theme	intestinal	1606:1615	arg1	samples					1628:1634	human intestinal microflora samples	1600:1634	human intestinal microflora samples	1600:1634	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	13	76	theme	CONCLUSION	2144:2153	arg1	S-3-1					2155:2159	CONCLUSION S-3-1	2144:2159	CONCLUSION S-3-1	2144:2159	CONCLUSION S-3-1 plays an immunomodulatory role by regulating the abundances of 9 intestinal bacteria genera.					
29890316	12	77	theme	incubated	1954:1962	arg1	S-3-1					1964:1968	incubated S-3-1	1954:1968	incubated S-3-1	1954:1968	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	16	78	theme	polysaccharides	2649:2663	arg1	mechanisms					2635:2644	the pharmacological mechanisms	2615:2644	the pharmacological mechanisms of polysaccharides	2615:2663	This study could provide new insights for studies on the pharmacological mechanisms of polysaccharides in vitro.					
29890316	6	79	dep	in	1089:1090	arg1	vitro					1092:1096	vitro	1092:1096	vitro	1092:1096	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	15	80	theme	flora	2489:2493	arg1	composition					2495:2505	the intestinal flora composition	2474:2505	the intestinal flora composition	2474:2505	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	9	81	dep	in	1383:1384	arg1	vitro					1386:1390	vitro	1386:1390	vitro	1386:1390	RESULTS S-3-1 was degraded in three in vitro fermentation models separately and sequentially.					
29890316	11	82	theme	sequential	1856:1865	arg1	incubation					1867:1876	sequential incubation	1856:1876	sequential incubation of S-3-1	1856:1885	Specifically, S-3-1 could only regulate the abundances of Paraprevotella and Oscillospira, while the influenced flora changed to Butyricimonas, Coprococcus, Dialister, Sutterella, Ruminococcus and Parabacteroides after sequential incubation of S-3-1.					
29890316	0	83	theme	human	66:70	arg1	microbes					83:90	human intestinal microbes	66:90	human intestinal microbes	66:90	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	12	84	from	effects	2056:2062	arg1	abundances					2071:2080	the abundances	2067:2080	the abundances of Enterococcus	2067:2096	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	84	from	effects	2056:2062	arg1	Butyricimonas					2111:2123	Butyricimonas	2111:2123	Butyricimonas	2111:2123	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	84	from	effects	2056:2062	arg1	Sutterella					2099:2108	Sutterella	2099:2108	Sutterella	2099:2108	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	12	84	from	effects	2056:2062	arg1	Streptococcus					2129:2141	Streptococcus	2129:2141	Streptococcus	2129:2141	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	1	85	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	RELEVANCE					151:159	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	85	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	recipe					199:204	a classic recipe	189:204	a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi	189:284	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	8	86	theme	rRNA	1295:1298	arg1	sequencing					1320:1329	rRNA gene Illumina MiSeq sequencing	1295:1329	rRNA gene Illumina MiSeq sequencing (V3-V4 region)	1295:1344	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	8	86	theme	rRNA	1295:1298	arg1	region					1338:1343	V3-V4 region	1332:1343	V3-V4 region	1332:1343	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	14	87	theme	kinds	2319:2323	arg1	genera					2297:2302	more bacteria genera	2283:2302	more bacteria genera	2283:2302	Incubated S-3-1 can regulate more bacteria genera, a total of 13 kinds, and can adjust the SCFA content to affect immunomodulation.					
29890316	14	87	theme	kinds	2319:2323	arg1	total					2307:2311	a total	2305:2311	a total of 13 kinds	2305:2323	Incubated S-3-1 can regulate more bacteria genera, a total of 13 kinds, and can adjust the SCFA content to affect immunomodulation.					
29890316	1	88	theme	Sijunzi	161:167	arg1	SJZD					180:183	SJZD	180:183	SJZD	180:183	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	88	theme	Sijunzi	161:167	arg1	decoction					169:177	Sijunzi decoction	161:177	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD)	130:184	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	12	89	from	influence	1920:1928	arg1	content					1937:1943	the content	1933:1943	the content of SCFA	1933:1951	In contrast to S-3-1 showing no influence on the content of SCFA, incubated S-3-1 showed increased contents of acetic acid and total acid that were associated with its effects on the abundances of Enterococcus, Sutterella, Butyricimonas and Streptococcus.					
29890316	8	90	theme	Illumina	1305:1312	arg1	sequencing					1320:1329	rRNA gene Illumina MiSeq sequencing	1295:1329	rRNA gene Illumina MiSeq sequencing (V3-V4 region)	1295:1344	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	8	90	theme	Illumina	1305:1312	arg1	region					1338:1343	V3-V4 region	1332:1343	V3-V4 region	1332:1343	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	0	91	theme	short	96:100	arg1	acids					114:118	short chain fatty acids	96:118	short chain fatty acids	96:118	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	15	92	theme	carbon	2547:2552	arg1	source					2554:2559	a carbon source	2545:2559	a carbon source	2545:2559	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	0	93	theme	fatty	108:112	arg1	acids					114:118	short chain fatty acids	96:118	short chain fatty acids	96:118	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	15	94	theme	intestinal	2414:2423	arg1	juices					2425:2430	gastric and intestinal juices	2402:2430	gastric and intestinal juices	2402:2430	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	8	95	theme	human	1242:1246	arg1	composition					1263:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition	1238:1273	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	4	96	theme	chain	760:764	arg1	acids					772:776	short chain fatty acids	754:776	short chain fatty acids	754:776	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	2	97	theme	poor	370:373	arg1	appetite					375:382	poor appetite	370:382	poor appetite	370:382	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	5	98	theme	METHODS	793:799	arg1	S-3-1					801:805	MATERIALS AND METHODS S-3-1	779:805	MATERIALS AND METHODS S-3-1	779:805	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	15	99	theme	gastric	2402:2408	arg1	juices					2425:2430	gastric and intestinal juices	2402:2430	gastric and intestinal juices	2402:2430	Incubation with gastric and intestinal juices enhanced S-3-1's capability of modulating the intestinal flora composition and decreased the bacteria's need for a carbon source.					
29890316	4	100	theme	STUDY	525:529	arg1	AIM					514:516	AIM	514:516	AIM OF THE STUDY	514:529	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	0	101	theme	homogeneous	13:23	arg1	polysaccharide					25:38	a homogeneous polysaccharide	11:38	a homogeneous polysaccharide from Sijunzi	11:51	Effects of a homogeneous polysaccharide from Sijunzi decoction on human intestinal microbes and short chain fatty acids in vitro.					
29890316	13	102	theme	genera	2246:2251	arg1	abundances					2210:2219	the abundances	2206:2219	the abundances of 9 intestinal bacteria genera	2206:2251	CONCLUSION S-3-1 plays an immunomodulatory role by regulating the abundances of 9 intestinal bacteria genera.					
29890316	6	103	theme	S-3-1	1074:1078	arg1	stability					1020:1028	the stability	1016:1028	the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models	1016:1116	The concentrations of total polysaccharide and reducing sugar were measured to identify the stability of independently and sequentially incubated S-3-1 in three in vitro fermentation models.					
29890316	4	104	theme	present	535:541	arg1	study					543:547	The present study	531:547	The present study	531:547	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	4	105	with	SJZD	653:656	arg1	activity					682:689	immune-enhancement activity	663:689	immune-enhancement activity	663:689	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	4	106	theme	intestinal	730:739	arg1	microbes					741:748	human intestinal microbes	724:748	human intestinal microbes	724:748	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	2	107	theme	food	393:396	arg1	intake					398:403	reduced food intake	385:403	reduced food intake	385:403	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	7	108	theme	acid	1193:1196	arg1	contents					1205:1212	the short chain fatty acid (SCFA) contents	1171:1212	the short chain fatty acid (SCFA) contents in human fecal samples	1171:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	5	109	theme	MATERIALS	779:787	arg1	S-3-1					801:805	MATERIALS AND METHODS S-3-1	779:805	MATERIALS AND METHODS S-3-1	779:805	MATERIALS AND METHODS S-3-1 was incubated with simulated gastric juice, intestinal juice, and human fecal microflora independently and sequentially.					
29890316	10	110	theme	Lactobacillus	1505:1517	arg1	abundances					1491:1500	the abundances	1487:1500	the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea	1487:1595	Both S-3-1 and incubated S-3-1 could regulate the abundances of Lactobacillus, Pediococcus, Streptococcus, Bacteroides, Enterococcus, Clostridium and Dorea in human intestinal microflora samples.					
29890316	11	111	theme	S-3-1	1881:1885	arg1	incubation					1867:1876	sequential incubation	1856:1876	sequential incubation of S-3-1	1856:1885	Specifically, S-3-1 could only regulate the abundances of Paraprevotella and Oscillospira, while the influenced flora changed to Butyricimonas, Coprococcus, Dialister, Sutterella, Ruminococcus and Parabacteroides after sequential incubation of S-3-1.					
29890316	4	112	from	effects	713:719	arg1	acids					772:776	short chain fatty acids	754:776	short chain fatty acids	754:776	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	4	112	from	effects	713:719	arg1	microbes					741:748	human intestinal microbes	724:748	human intestinal microbes	724:748	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	13	113	theme	intestinal	2226:2235	arg1	genera					2246:2251	9 intestinal bacteria genera	2224:2251	9 intestinal bacteria genera	2224:2251	CONCLUSION S-3-1 plays an immunomodulatory role by regulating the abundances of 9 intestinal bacteria genera.					
29890316	4	114	theme	immunomodulatory	573:588	arg1	mechanism					590:598	the immunomodulatory mechanism	569:598	the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity,	569:690	AIM OF THE STUDY The present study aimed to understand the immunomodulatory mechanism of S-3-1, a homogeneous polysaccharide purified from SJZD with immune-enhancement activity, by investigating its effects on human intestinal microbes and short chain fatty acids.					
29890316	8	115	theme	V3-V4	1332:1336	arg1	sequencing					1320:1329	rRNA gene Illumina MiSeq sequencing	1295:1329	rRNA gene Illumina MiSeq sequencing (V3-V4 region)	1295:1344	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	8	115	theme	V3-V4	1332:1336	arg1	region					1338:1343	V3-V4 region	1332:1343	V3-V4 region	1332:1343	The human gut microbiota composition was measured by 16S rRNA gene Illumina MiSeq sequencing (V3-V4 region).					
29890316	11	116	theme	Oscillospira	1714:1725	arg1	abundances					1681:1690	the abundances	1677:1690	the abundances of Paraprevotella and Oscillospira	1677:1725	Specifically, S-3-1 could only regulate the abundances of Paraprevotella and Oscillospira, while the influenced flora changed to Butyricimonas, Coprococcus, Dialister, Sutterella, Ruminococcus and Parabacteroides after sequential incubation of S-3-1.					
29890316	2	117	theme	gastrointestinal	330:345	arg1	function					347:354	gastrointestinal function	330:354	gastrointestinal function manifested in poor appetite, reduced food intake and loose stools	330:420	It is well known for treating disorders of gastrointestinal function manifested in poor appetite, reduced food intake and loose stools.					
29890316	1	118	theme	Chinese	221:227	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine (TCM)	209:242	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	1	118	theme	Chinese	221:227	arg1	TCM					239:241	TCM	239:241	TCM	239:241	ETHNOPHARMACOLOGICAL RELEVANCE Sijunzi decoction (SJZD) is a classic recipe in traditional Chinese medicine (TCM) to strengthen the spleen and replenish Qi.					
29890316	7	119	theme	short	1175:1179	arg1	SCFA					1199:1202	SCFA	1199:1202	SCFA	1199:1202	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29890316	7	119	theme	short	1175:1179	arg1	acid					1193:1196	the short chain fatty acid	1171:1196	the short chain fatty acid (SCFA) contents in human fecal samples	1171:1235	Gas chromatograph (GC) analysis was used to measure the short chain fatty acid (SCFA) contents in human fecal samples.					
29802725	10	0	theme	QSG	1465:1467	arg1	behavior					1449:1456	The rheological behavior	1433:1456	The rheological behavior of the QSG studied in this paper	1433:1489	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	6	1	from	decrease	974:981	arg1	viscosity					996:1004	intrinsic viscosity	986:1004	intrinsic viscosity of QSG	986:1011	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	8	2	theme	apparent	1262:1269	arg1	viscosity					1271:1279	apparent viscosity	1262:1279	apparent viscosity	1262:1279	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	0	3	theme	quince	86:91	arg1	seeds					93:97	quince seeds	86:97	quince seeds	86:97	Characterizations and rheological study of the purified polysaccharide extracted from quince seeds.					
29802725	7	4	theme	consistency	1080:1090	arg1	coefficient					1092:1102	the consistency coefficient	1076:1102	the consistency coefficient (as a measure of apparent viscosity)	1076:1139	When the salt concentration increased from 0 to 50 mmol L-1 , the consistency coefficient (as a measure of apparent viscosity) declined.					
29802725	1	5	dep	BACKGROUND	100:109	arg1	dependent					170:178	dependent	170:178	dependent	170:178	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	1	5	dep	BACKGROUND	100:109	arg1	characteristics					126:140	The functional characteristics	111:140	The functional characteristics of hydrocolloids	111:157	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	3	6	theme	5.64 ± 0.21	589:599	arg1	%					600:600	%	600:600	%	600:600	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	7	theme	13.16 ± 1.73	517:528	arg1	%					529:529	%	529:529	%	529:529	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	8	dep	carbohydrate	409:420	arg1	%					482:482	5.75% d-glucose	478:492	5.75% d-glucose	478:492	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	8	dep	carbohydrate	409:420	arg1	%					427:427	6.39% l-arabinose	423:439	6.39% l-arabinose	423:439	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	8	dep	carbohydrate	409:420	arg1	d-xylose					449:456	40.43% d-xylose	442:456	40.43% d-xylose	442:456	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	8	dep	carbohydrate	409:420	arg1	%					503:503	31.11% d-mannose	498:513	31.11% d-mannose	498:513	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	8	dep	carbohydrate	409:420	arg1	%					463:463	5.60%	459:463	5.60% d-galactose	459:475	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	9	theme	%	529:529	arg1	acid					538:541	13.16 ± 1.73% uronic acid	517:541	13.16 ± 1.73% uronic acid	517:541	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	10	10	theme	potential	1520:1528	arg1	application					1530:1540	its potential application	1516:1540	its potential application in food and pharmaceutical industries	1516:1578	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	9	11	theme	control	1405:1411	arg1	samples					1413:1419	control samples	1405:1419	control samples	1405:1419	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
29802725	11	12	theme	Chemical	1599:1606	arg1	Industry					1608:1615	Chemical Industry	1599:1615	Chemical Industry	1599:1615	© 2018 Society of Chemical Industry.					
29802725	4	13	from	by-product	703:712	arg1	industries					745:754	the food and pharmaceutical industries	717:754	the food and pharmaceutical industries	717:754	Our findings indicated that this gum could be introduced as a value-added by-product in the food and pharmaceutical industries.					
29802725	4	14	theme	food	721:724	arg1	industries					745:754	the food and pharmaceutical industries	717:754	the food and pharmaceutical industries	717:754	Our findings indicated that this gum could be introduced as a value-added by-product in the food and pharmaceutical industries.					
29802725	1	15	theme	physicochemical	189:203	arg1	properties					205:214	their physicochemical properties	183:214	their physicochemical properties	183:214	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	10	16	from	application	1530:1540	arg1	industries					1569:1578	food and pharmaceutical industries	1545:1578	food and pharmaceutical industries	1545:1578	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	5	17	theme	Carbon-13	757:765	arg1	resonance					784:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	7	18	theme	viscosity	1130:1138	arg1	measure					1110:1116	a measure	1108:1116	a measure of apparent viscosity	1108:1138	When the salt concentration increased from 0 to 50 mmol L-1 , the consistency coefficient (as a measure of apparent viscosity) declined.					
29802725	9	19	theme	G	1315:1315	arg1	values					1325:1330	the G' and G″ values	1311:1330	the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations	1311:1385	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
29802725	3	20	theme	%	407:407	arg1	carbohydrate					409:420	85.04 ± 2.87% carbohydrate	395:420	85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose)	395:514	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	21	theme	high-molecular-weight	324:344	arg1	polysaccharide					346:359	a high-molecular-weight polysaccharide	322:359	a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat	322:626	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	21	theme	high-molecular-weight	324:344	arg1	g mol-1					373:379	9.61 × 106 g mol-1	362:379	9.61 × 106 g mol-1	362:379	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	21	theme	high-molecular-weight	324:344	arg1	gum					309:311	Quince seed gum	297:311	Quince seed gum (QSG)	297:317	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	7	22	theme	apparent	1121:1128	arg1	viscosity					1130:1138	apparent viscosity	1121:1138	apparent viscosity	1121:1138	When the salt concentration increased from 0 to 50 mmol L-1 , the consistency coefficient (as a measure of apparent viscosity) declined.					
29802725	9	23	theme	chloride	1363:1370	arg1	concentrations					1372:1385	calcium chloride concentrations	1355:1385	calcium chloride concentrations	1355:1385	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
29802725	5	24	theme	nuclear	767:773	arg1	resonance					784:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	6	25	theme	ion	935:937	arg1	concentration					939:951	the ion concentration	931:951	the ion concentration	931:951	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	3	26	theme	5.77 ± 0.83	544:554	arg1	%					555:555	%	555:555	%	555:555	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	8	27	theme	salt	1197:1200	arg1	concentration					1202:1214	salt concentration	1197:1214	salt concentration	1197:1214	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	3	28	theme	40.43	442:446	arg1	%					447:447	%	447:447	%	447:447	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	5	29	theme	magnetic	775:782	arg1	resonance					784:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance	757:792	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	3	30	dep	%	463:463	arg1	d-galactose					465:475	d-galactose	465:475	5.60% d-galactose	459:475	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	31	theme	seed	304:307	arg1	polysaccharide					346:359	a high-molecular-weight polysaccharide	322:359	a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat	322:626	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	31	theme	seed	304:307	arg1	QSG					314:316	QSG	314:316	QSG	314:316	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	31	theme	seed	304:307	arg1	gum					309:311	Quince seed gum	297:311	Quince seed gum (QSG)	297:317	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	32	theme	uronic	531:536	arg1	acid					538:541	13.16 ± 1.73% uronic acid	517:541	13.16 ± 1.73% uronic acid	517:541	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	33	theme	85.04 ± 2.87	395:406	arg1	%					407:407	%	407:407	%	407:407	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	6	34	from	increase	919:926	arg1	concentration					939:951	the ion concentration	931:951	the ion concentration	931:951	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	1	35	theme	functional	115:124	arg1	dependent					170:178	dependent	170:178	dependent	170:178	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	1	35	theme	functional	115:124	arg1	characteristics					126:140	The functional characteristics	111:140	The functional characteristics of hydrocolloids	111:157	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	6	36	theme	QSG	1009:1011	arg1	viscosity					996:1004	intrinsic viscosity	986:1004	intrinsic viscosity of QSG	986:1011	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	8	37	theme	other	1158:1162	arg1	hand					1164:1167	the other hand	1154:1167	the other hand	1154:1167	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	8	38	theme	consistency	1221:1231	arg1	values					1282:1287	the consistency coefficient (as a measure of apparent viscosity) values	1217:1287	the consistency coefficient (as a measure of apparent viscosity) values	1217:1287	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	2	39	theme	new	259:261	arg1	sources					263:269	the new sources	255:269	the new sources of hydrocolloids	255:286	Thus, it is essential to characterize the new sources of hydrocolloids.					
29802725	10	40	theme	pharmaceutical	1554:1567	arg1	industries					1569:1578	food and pharmaceutical industries	1545:1578	food and pharmaceutical industries	1545:1578	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	3	41	theme	l-arabinose	429:439	arg1	%					427:427	6.39% l-arabinose	423:439	6.39% l-arabinose	423:439	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	42	theme	Quince	297:302	arg1	polysaccharide					346:359	a high-molecular-weight polysaccharide	322:359	a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat	322:626	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	42	theme	Quince	297:302	arg1	QSG					314:316	QSG	314:316	QSG	314:316	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	42	theme	Quince	297:302	arg1	gum					309:311	Quince seed gum	297:311	Quince seed gum (QSG)	297:317	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	43	theme	%	600:600	arg1	ash					602:604	5.64 ± 0.21% ash	589:604	5.64 ± 0.21% ash	589:604	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	44	theme	2.78 ± 0.21	567:577	arg1	%					578:578	%	578:578	%	578:578	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	6	45	theme	dilute	901:906	arg1	regime					908:913	the dilute regime	897:913	the dilute regime	897:913	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	0	46	theme	polysaccharide	56:69	arg1	Characterizations					0:16	Characterizations	0:16	Characterizations	0:16	Characterizations and rheological study of the purified polysaccharide extracted from quince seeds.					
29802725	0	46	theme	polysaccharide	56:69	arg1	study					34:38	rheological study	22:38	rheological study	22:38	Characterizations and rheological study of the purified polysaccharide extracted from quince seeds.					
29802725	3	47	theme	%	447:447	arg1	d-xylose					449:456	40.43% d-xylose	442:456	40.43% d-xylose	442:456	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	48	theme	%	555:555	arg1	moisture					557:564	5.77 ± 0.83% moisture	544:564	5.77 ± 0.83% moisture	544:564	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	8	49	theme	coefficient	1233:1243	arg1	values					1282:1287	the consistency coefficient (as a measure of apparent viscosity) values	1217:1287	the consistency coefficient (as a measure of apparent viscosity) values	1217:1287	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	0	50	theme	purified	47:54	arg1	polysaccharide					56:69	the purified polysaccharide	43:69	the purified polysaccharide	43:69	Characterizations and rheological study of the purified polysaccharide extracted from quince seeds.					
29802725	7	51	theme	salt	1023:1026	arg1	concentration					1028:1040	the salt concentration	1019:1040	the salt concentration	1019:1040	When the salt concentration increased from 0 to 50 mmol L-1 , the consistency coefficient (as a measure of apparent viscosity) declined.					
29802725	9	52	dep	10	1336:1337	arg1	concentrations					1372:1385	calcium chloride concentrations	1355:1385	calcium chloride concentrations	1355:1385	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
29802725	3	53	theme	d-glucose	484:492	arg1	%					482:482	5.75% d-glucose	478:492	5.75% d-glucose	478:492	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	5	54	dep	Fourier	798:804	arg1	transform					806:814	transform	806:814	transform infrared spectroscopy	806:836	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	10	55	theme	rheological	1437:1447	arg1	behavior					1449:1456	The rheological behavior	1433:1456	The rheological behavior of the QSG studied in this paper	1433:1489	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	5	56	dep	transform	806:814	arg1	infrared					816:823	infrared	816:823	transform infrared spectroscopy	806:836	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	4	57	theme	pharmaceutical	730:743	arg1	industries					745:754	the food and pharmaceutical industries	717:754	the food and pharmaceutical industries	717:754	Our findings indicated that this gum could be introduced as a value-added by-product in the food and pharmaceutical industries.					
29802725	1	58	theme	hydrocolloids	145:157	arg1	dependent					170:178	dependent	170:178	dependent	170:178	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	1	58	theme	hydrocolloids	145:157	arg1	characteristics					126:140	The functional characteristics	111:140	The functional characteristics of hydrocolloids	111:157	BACKGROUND The functional characteristics of hydrocolloids are mainly dependent on their physicochemical properties.					
29802725	4	59	theme	value-added	691:701	arg1	gum					662:664	this gum	657:664	this gum	657:664	Our findings indicated that this gum could be introduced as a value-added by-product in the food and pharmaceutical industries.					
29802725	4	59	theme	value-added	691:701	arg1	by-product					703:712	a value-added by-product	689:712	a value-added by-product in the food and pharmaceutical industries	689:754	Our findings indicated that this gum could be introduced as a value-added by-product in the food and pharmaceutical industries.					
29802725	6	60	theme	intrinsic	986:994	arg1	viscosity					996:1004	intrinsic viscosity	986:1004	intrinsic viscosity of QSG	986:1011	In the dilute regime, an increase in the ion concentration was accompanied by a decrease in intrinsic viscosity of QSG.					
29802725	3	61	theme	0.75 ± 0.09	611:621	arg1	%					622:622	%	622:622	%	622:622	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	2	62	theme	hydrocolloids	274:286	arg1	sources					263:269	the new sources	255:269	the new sources of hydrocolloids	255:286	Thus, it is essential to characterize the new sources of hydrocolloids.					
29802725	5	63	theme	xylan	869:873	arg1	structure					875:883	a highly substituted xylan structure	848:883	a highly substituted xylan structure for QSG	848:891	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	8	64	theme	viscosity	1271:1279	arg1	measure					1251:1257	a measure	1249:1257	a measure of apparent viscosity	1249:1279	On the other hand, with further increasing of salt concentration, the consistency coefficient (as a measure of apparent viscosity) values increased.					
29802725	3	65	dep	RESULTS	289:295	arg1	polysaccharide					346:359	a high-molecular-weight polysaccharide	322:359	a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat	322:626	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	65	dep	RESULTS	289:295	arg1	g mol-1					373:379	9.61 × 106 g mol-1	362:379	9.61 × 106 g mol-1	362:379	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	65	dep	RESULTS	289:295	arg1	gum					309:311	Quince seed gum	297:311	Quince seed gum (QSG)	297:317	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	66	theme	%	622:622	arg1	fat					624:626	0.75 ± 0.09% fat	611:626	0.75 ± 0.09% fat	611:626	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	3	67	theme	%	578:578	arg1	protein					580:586	2.78 ± 0.21% protein	567:586	2.78 ± 0.21% protein	567:586	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	5	68	theme	substituted	857:867	arg1	structure					875:883	a highly substituted xylan structure	848:883	a highly substituted xylan structure for QSG	848:891	Carbon-13 nuclear magnetic resonance and Fourier transform infrared spectroscopy suggested a highly substituted xylan structure for QSG.					
29802725	9	69	theme	calcium	1355:1361	arg1	concentrations					1372:1385	calcium chloride concentrations	1355:1385	calcium chloride concentrations	1355:1385	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
29802725	0	70	theme	rheological	22:32	arg1	study					34:38	rheological study	22:38	rheological study	22:38	Characterizations and rheological study of the purified polysaccharide extracted from quince seeds.					
29802725	10	71	dep	CONCLUSION	1422:1431	arg1	provide					1495:1501	provide	1495:1501	can provide insight into its potential application in food and pharmaceutical industries	1491:1578	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	3	72	theme	d-mannose	505:513	arg1	%					503:503	31.11% d-mannose	498:513	31.11% d-mannose	498:513	RESULTS Quince seed gum (QSG) is a high-molecular-weight polysaccharide (9.61 × 106 g mol-1 ) composed of 85.04 ± 2.87% carbohydrate (6.39% l-arabinose, 40.43% d-xylose, 5.60% d-galactose, 5.75% d-glucose and 31.11% d-mannose), 13.16 ± 1.73% uronic acid, 5.77 ± 0.83% moisture, 2.78 ± 0.21% protein, 5.64 ± 0.21% ash, and 0.75 ± 0.09% fat.					
29802725	10	73	theme	food	1545:1548	arg1	industries					1569:1578	food and pharmaceutical industries	1545:1578	food and pharmaceutical industries	1545:1578	CONCLUSION The rheological behavior of the QSG studied in this paper can provide insight into its potential application in food and pharmaceutical industries.					
29802725	9	74	theme	G″	1322:1323	arg1	values					1325:1330	the G' and G″ values	1311:1330	the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations	1311:1385	Similarly, the G' and G″ values for 10 and 50 mmol L-1 calcium chloride concentrations were less than in control samples.					
30473522	0	0	theme	Flammulina	96:105	arg1	velutipes					107:115	Flammulina velutipes	96:115	Flammulina velutipes on Caenorhabditis elegans	96:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	4	1	theme	optimal	736:742	arg1	conditions					744:753	optimal conditions	736:753	optimal conditions	736:753	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	2	2	theme	microwave	426:434	arg1	time					436:439	microwave time	426:439	microwave time	426:439	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	8	3	theme	anti-ultraviolet	1179:1194	arg1	activity					1196:1203	certain anti-ultraviolet activity	1171:1203	certain anti-ultraviolet activity	1171:1203	Moreover, FRP-2 exhibited certain anti-ultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL.					
30473522	1	4	theme	anti-ultraviolet	198:213	arg1	activity					215:222	anti-ultraviolet activity	198:222	anti-ultraviolet activity	198:222	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	6	5	theme	FRP-2	976:980	arg1	weight					966:971	The average mean molecular weight	939:971	The average mean molecular weight of FRP-2	939:980	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	6	5	theme	FRP-2	976:980	arg1	Da					1010:1011	2.60×105 Da	1001:1011	2.60×105 Da	1001:1011	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	3	6	theme	extraction	617:626	arg1	conditions					628:637	the following optimal extraction conditions	595:637	the following optimal extraction conditions	595:637	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	0	7	theme	velutipes	107:115	arg1	root					88:91	the root	84:91	the root of Flammulina velutipes on Caenorhabditis elegans	84:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	8	theme	following	599:607	arg1	conditions					628:637	the following optimal extraction conditions	595:637	the following optimal extraction conditions	595:637	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	4	9	theme	measured	767:774	arg1	%					803:803	2.01%	799:803	2.01% (w/w)	799:809	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	4	9	theme	measured	767:774	arg1	value					776:780	the actual measured value	756:780	the actual measured value of the yield	756:793	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	1	10	from	extraction	183:192	arg1	root					266:269	the root	262:269	the root of Flammulina velutipes on Caenorhabditis elegans	262:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	11	theme	root	589:592	arg1	yield					567:571	the polysaccharide yield	548:571	the polysaccharide yield of F. velutipes root	548:592	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	2	12	theme	microwave	446:454	arg1	power					456:460	microwave power	446:460	microwave power	446:460	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	0	13	theme	Caenorhabditis	120:133	arg1	elegans					135:141	Caenorhabditis elegans	120:141	Caenorhabditis elegans	120:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	7	14	theme	sugar	1072:1076	arg1	present					1088:1094	present	1088:1094	present	1088:1094	The sugar units are present both in the α-configuration and β-configuration.					
30473522	7	14	theme	sugar	1072:1076	arg1	units					1078:1082	The sugar units	1068:1082	The sugar units	1068:1082	The sugar units are present both in the α-configuration and β-configuration.					
30473522	7	14	theme	sugar	1072:1076	arg1	both					1096:1099	both	1096:1099	both	1096:1099	The sugar units are present both in the α-configuration and β-configuration.					
30473522	2	15	theme	design	336:341	arg1	experiment					343:352	A Box-Behnken design experiment	322:352	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power,	322:461	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	16	dep	results	496:502	arg1	basis					483:487	basis	483:487	basis	483:487	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	16	dep	results	496:502	arg1	the					479:481	the	479:481	the	479:481	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	8	17	theme	polysaccharide	1228:1241	arg1	concentration					1243:1255	the polysaccharide concentration	1224:1255	the polysaccharide concentration	1224:1255	Moreover, FRP-2 exhibited certain anti-ultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL.					
30473522	1	18	theme	water-soluble	227:239	arg1	polysaccharides					241:255	water-soluble polysaccharides	227:255	water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans	227:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	19	theme	Cellulase-assisted	0:17	arg1	extraction					19:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	6	20	theme	molecular	956:964	arg1	weight					966:971	The average mean molecular weight	939:971	The average mean molecular weight of FRP-2	939:980	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	6	20	theme	molecular	956:964	arg1	Da					1010:1011	2.60×105 Da	1001:1011	2.60×105 Da	1001:1011	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	3	21	theme	microwave	713:721	arg1	power					723:727	144 W microwave power	707:727	144 W microwave power	707:727	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	1	22	theme	polysaccharides	241:255	arg1	extraction					183:192	cellulase-assisted extraction	164:192	cellulase-assisted extraction	164:192	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	1	22	theme	polysaccharides	241:255	arg1	activity					215:222	anti-ultraviolet activity	198:222	anti-ultraviolet activity	198:222	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	23	theme	anti-ultraviolet	34:49	arg1	activity					51:58	anti-ultraviolet activity	34:58	anti-ultraviolet activity	34:58	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	6	24	theme	mean	951:954	arg1	weight					966:971	The average mean molecular weight	939:971	The average mean molecular weight of FRP-2	939:980	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	6	24	theme	mean	951:954	arg1	Da					1010:1011	2.60×105 Da	1001:1011	2.60×105 Da	1001:1011	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	3	25	theme	optimal	609:615	arg1	conditions					628:637	the following optimal extraction conditions	595:637	the following optimal extraction conditions	595:637	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	7	26	dep	present	1088:1094	arg1	present					1088:1094	present	1088:1094	present	1088:1094	The sugar units are present both in the α-configuration and β-configuration.					
30473522	7	26	dep	present	1088:1094	arg1	units					1078:1082	The sugar units	1068:1082	The sugar units	1068:1082	The sugar units are present both in the α-configuration and β-configuration.					
30473522	7	26	dep	present	1088:1094	arg1	both					1096:1099	both	1096:1099	both	1096:1099	The sugar units are present both in the α-configuration and β-configuration.					
30473522	6	27	theme	average	943:949	arg1	weight					966:971	The average mean molecular weight	939:971	The average mean molecular weight of FRP-2	939:980	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	6	27	theme	average	943:949	arg1	Da					1010:1011	2.60×105 Da	1001:1011	2.60×105 Da	1001:1011	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	1	28	dep	extraction	183:192	arg1	the					160:162	the	160:162	the	160:162	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	29	theme	F.	576:577	arg1	root					589:592	F. velutipes root	576:592	F. velutipes root	576:592	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	30	used	used	644:647	arg2	time					697:700	80s microwave time	683:700	80s microwave time	683:700	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	30	used	used	644:647	arg2	conditions					628:637	the following optimal extraction conditions	595:637	the following optimal extraction conditions	595:637	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	30	used	used	644:647	arg2	temperature					670:680	52.67°C enzymolysis temperature	650:680	52.67°C enzymolysis temperature	650:680	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	30	used	used	644:647	arg2	power					723:727	144 W microwave power	707:727	144 W microwave power	707:727	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	1	31	from	elegans	313:319	arg1	root					266:269	the root	262:269	the root of Flammulina velutipes on Caenorhabditis elegans	262:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	32	theme	144	707:709	arg1	W					711:711	W	711:711	W	711:711	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	33	theme	52.67°C	650:656	arg1	temperature					670:680	52.67°C enzymolysis temperature	650:680	52.67°C enzymolysis temperature	650:680	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	6	34	theme	monosaccharide	1022:1035	arg1	composition					1037:1047	its monosaccharide composition	1018:1047	its monosaccharide composition	1018:1047	The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose.					
30473522	2	35	theme	experiments	521:531	arg1	results					496:502	the results	492:502	the results of single-factor experiments	492:531	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	0	36	from	root	88:91	arg1	extraction					19:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	36	from	root	88:91	arg1	activity					51:58	anti-ultraviolet activity	34:58	anti-ultraviolet activity	34:58	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	36	from	root	88:91	arg1	elegans					135:141	Caenorhabditis elegans	120:141	Caenorhabditis elegans	120:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	37	theme	enzymolysis	658:668	arg1	temperature					670:680	52.67°C enzymolysis temperature	650:680	52.67°C enzymolysis temperature	650:680	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	1	38	from	root	266:269	arg1	extraction					183:192	cellulase-assisted extraction	164:192	cellulase-assisted extraction	164:192	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	1	38	from	root	266:269	arg1	activity					215:222	anti-ultraviolet activity	198:222	anti-ultraviolet activity	198:222	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	1	38	from	root	266:269	arg1	polysaccharides					241:255	water-soluble polysaccharides	227:255	water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans	227:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	1	38	from	root	266:269	arg1	elegans					313:319	Caenorhabditis elegans	298:319	Caenorhabditis elegans	298:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	2	39	theme	single-factor	507:519	arg1	experiments					521:531	single-factor experiments	507:531	single-factor experiments	507:531	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	4	40	theme	actual	760:765	arg1	%					803:803	2.01%	799:803	2.01% (w/w)	799:809	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	4	40	theme	actual	760:765	arg1	value					776:780	the actual measured value	756:780	the actual measured value of the yield	756:793	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	0	41	theme	polysaccharides	63:77	arg1	extraction					19:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction	0:28	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	41	theme	polysaccharides	63:77	arg1	activity					51:58	anti-ultraviolet activity	34:58	anti-ultraviolet activity	34:58	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	42	from	elegans	135:141	arg1	root					88:91	the root	84:91	the root of Flammulina velutipes on Caenorhabditis elegans	84:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	2	43	theme	enzymolysis	401:411	arg1	temperature					413:423	enzymolysis temperature	401:423	enzymolysis temperature	401:423	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	3	44	theme	80s	683:685	arg1	time					697:700	80s microwave time	683:700	80s microwave time	683:700	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	2	45	theme	Box-Behnken	324:334	arg1	experiment					343:352	A Box-Behnken design experiment	322:352	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power,	322:461	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	1	46	theme	Flammulina	274:283	arg1	velutipes					285:293	Flammulina velutipes	274:293	Flammulina velutipes on Caenorhabditis elegans	274:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	1	47	from	velutipes	285:293	arg1	elegans					313:319	Caenorhabditis elegans	298:319	Caenorhabditis elegans	298:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	3	48	theme	W	711:711	arg1	power					723:727	144 W microwave power	707:727	144 W microwave power	707:727	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	3	49	theme	microwave	687:695	arg1	time					697:700	80s microwave time	683:700	80s microwave time	683:700	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	4	50	theme	yield	789:793	arg1	%					803:803	2.01%	799:803	2.01% (w/w)	799:809	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	4	50	theme	yield	789:793	arg1	value					776:780	the actual measured value	756:780	the actual measured value of the yield	756:793	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	3	51	theme	velutipes	579:587	arg1	root					589:592	F. velutipes root	576:592	F. velutipes root	576:592	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	1	52	theme	velutipes	285:293	arg1	root					266:269	the root	262:269	the root of Flammulina velutipes on Caenorhabditis elegans	262:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	0	53	from	velutipes	107:115	arg1	elegans					135:141	Caenorhabditis elegans	120:141	Caenorhabditis elegans	120:141	Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	4	54	theme	predicted	819:827	arg1	value					829:833	the predicted value	815:833	the predicted value	815:833	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	4	54	theme	predicted	819:827	arg1	%					843:843	2.06%	839:843	2.06% (w/w)	839:849	Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w).					
30473522	1	55	theme	cellulase-assisted	164:181	arg1	extraction					183:192	cellulase-assisted extraction	164:192	cellulase-assisted extraction	164:192	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	8	56	theme	certain	1171:1177	arg1	activity					1196:1203	certain anti-ultraviolet activity	1171:1203	certain anti-ultraviolet activity	1171:1203	Moreover, FRP-2 exhibited certain anti-ultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL.					
30473522	1	57	from	activity	215:222	arg1	root					266:269	the root	262:269	the root of Flammulina velutipes on Caenorhabditis elegans	262:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
30473522	2	58	with	experiment	343:352	arg1	power					456:460	microwave power	446:460	microwave power	446:460	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	58	with	experiment	343:352	arg1	temperature					413:423	enzymolysis temperature	401:423	enzymolysis temperature	401:423	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	58	with	experiment	343:352	arg1	time					436:439	microwave time	426:439	microwave time	426:439	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	58	with	experiment	343:352	arg1	factors					365:371	three factors	359:371	three factors	359:371	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	2	58	with	experiment	343:352	arg1	levels					383:388	three levels	377:388	three levels	377:388	A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments.					
30473522	3	59	theme	polysaccharide	552:565	arg1	yield					567:571	the polysaccharide yield	548:571	the polysaccharide yield of F. velutipes root	548:592	For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power.					
30473522	1	60	theme	Caenorhabditis	298:311	arg1	elegans					313:319	Caenorhabditis elegans	298:319	Caenorhabditis elegans	298:319	We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.					
31089153	0	0	theme	Navicula	72:79	arg1	sp					81:82	diatom Navicula sp	65:82	diatom Navicula sp	65:82	Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp.					
31089153	6	1	from	strands	945:951	arg1	force					977:981	the maximum adhesive force	956:981	the maximum adhesive force	956:981	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	1	from	strands	945:951	arg1	different					921:929	different	921:929	different	921:929	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	1	from	strands	945:951	arg1	length					1017:1022	the maximum polymer extension length	987:1022	the maximum polymer extension length	987:1022	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	1	from	strands	945:951	arg1	properties					880:889	the mechanical properties	865:889	the mechanical properties of mucilage trails	865:908	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	1	2	theme	sp	137:138	arg1	trails					107:112	The secreted mucilage trails	85:112	The secreted mucilage trails of the diatom Navicula sp	85:138	The secreted mucilage trails of the diatom Navicula sp.					
31089153	0	3	theme	diatom	65:70	arg1	sp					81:82	diatom Navicula sp	65:82	diatom Navicula sp	65:82	Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp.					
31089153	8	4	theme	diatom	1378:1383	arg1	motility					1385:1392	diatom motility	1378:1392	diatom motility	1378:1392	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	8	5	theme	precise	1302:1308	arg1	information					1310:1320	more precise information	1297:1320	more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention	1297:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	7	6	contain	contained	1178:1186	arg2	components					1199:1208	some other components	1188:1208	some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches	1188:1268	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	6	contain	contained	1178:1186	arg1	strands					1170:1176	the mucilage strands	1157:1176	the mucilage strands	1157:1176	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	6	7	theme	adhesive	968:975	arg1	force					977:981	the maximum adhesive force	956:981	the maximum adhesive force	956:981	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	4	8	theme	increased	507:515	arg1	peak					531:534	an increased tip-substrate peak	504:534	an increased tip-substrate peak	504:534	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	0	9	theme	sp	81:82	arg1	mobility					53:60	the mobility	49:60	the mobility of diatom Navicula sp	49:82	Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp.					
31089153	2	10	theme	Raman	294:298	arg1	spectra					300:306	Raman spectra	294:306	Raman spectra	294:306	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	6	11	theme	mucilage	936:943	arg1	strands					945:951	mucilage strands	936:951	mucilage strands in the maximum adhesive force and the maximum polymer extension length	936:1022	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	7	12	theme	mucilages	1110:1118	arg1	kinds					1101:1105	the two kinds	1093:1105	the two kinds of mucilages	1093:1118	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	13	theme	Raman	1025:1029	arg1	spectra					1031:1037	Raman spectra	1025:1037	Raman spectra	1025:1037	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	4	14	theme	small	542:546	arg1	peak					579:582	a small saw-tooth pattern tip-mucilage peak	540:582	a small saw-tooth pattern tip-mucilage peak	540:582	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	3	15	theme	force	445:449	arg1	method					427:432	the method	423:432	the method of in situ force mapping by AFM	423:464	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	7	16	theme	asymmetric	1235:1244	arg1	stretches					1260:1268	asymmetric and symmetric stretches	1235:1268	asymmetric and symmetric stretches	1235:1268	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	6	17	theme	extension	1007:1015	arg1	length					1017:1022	the maximum polymer extension length	987:1022	the maximum polymer extension length	987:1022	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	18	theme	maximum	960:966	arg1	force					977:981	the maximum adhesive force	956:981	the maximum adhesive force	956:981	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	7	19	with	components	1199:1208	arg1	-CH3					1230:1233	-CH3	1230:1233	-CH3	1230:1233	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	19	with	components	1199:1208	arg1	-CH2-					1220:1224	-CH2-	1220:1224	-CH2-	1220:1224	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	19	with	components	1199:1208	arg1	C=O					1215:1217	C=O	1215:1217	C=O	1215:1217	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	2	20	theme	electron	233:240	arg1	TEM					254:256	TEM	254:256	TEM	254:256	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	20	theme	electron	233:240	arg1	microscope					242:251	transmission electron microscope	220:251	transmission electron microscope (TEM)	220:257	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	6	21	theme	polymer	999:1005	arg1	length					1017:1022	the maximum polymer extension length	987:1022	the maximum polymer extension length	987:1022	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	8	22	from	terms	1369:1373	arg1	useful					1359:1364	useful	1359:1364	useful	1359:1364	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	7	23	dep	C=O	1215:1217	arg1	stretches					1260:1268	asymmetric and symmetric stretches	1235:1268	asymmetric and symmetric stretches	1235:1268	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	2	24	theme	force	267:271	arg1	AFM					285:287	AFM	285:287	AFM	285:287	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	24	theme	force	267:271	arg1	microscopy					273:282	atomic force microscopy	260:282	atomic force microscopy (AFM)	260:288	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	6	25	theme	maximum	991:997	arg1	length					1017:1022	the maximum polymer extension length	987:1022	the maximum polymer extension length	987:1022	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	3	26	theme	previous	325:332	arg1	studies					334:340	previous studies	325:340	previous studies	325:340	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	6	27	theme	trails	903:908	arg1	different					921:929	different	921:929	different	921:929	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	27	theme	trails	903:908	arg1	properties					880:889	the mechanical properties	865:889	the mechanical properties of mucilage trails	865:908	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	28	from	different	921:929	arg1	addition					855:862	addition	855:862	addition	855:862	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	2	29	theme	atomic	260:265	arg1	AFM					285:287	AFM	285:287	AFM	285:287	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	29	theme	atomic	260:265	arg1	microscopy					273:282	atomic force microscopy	260:282	atomic force microscopy (AFM)	260:288	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	5	30	theme	adhesive	748:755	arg1	force					757:761	the adhesive force	744:761	the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion	744:849	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	3	31	dep	in	437:438	arg1	situ					440:443	situ	440:443	situ	440:443	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	8	32	theme	mucilage	1328:1335	arg1	trails					1337:1342	mucilage trails	1328:1342	mucilage trails which would be useful in terms of diatom motility and biofouling prevention	1328:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	2	33	dep	microscopy	202:211	arg1	etc					308:310	etc	308:310	etc	308:310	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	0	34	theme	frustules	16:24	arg1	Contribution					0:11	Contribution	0:11	Contribution of frustules and mucilage	0:37	Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp.					
31089153	4	35	theme	force	482:486	arg1	curve					488:492	The retraction force curve	467:492	The retraction force curve	467:492	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	8	36	theme	prevention	1409:1418	arg1	terms					1369:1373	terms	1369:1373	terms of diatom motility and biofouling prevention	1369:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	1	37	theme	secreted	89:96	arg1	trails					107:112	The secreted mucilage trails	85:112	The secreted mucilage trails of the diatom Navicula sp	85:138	The secreted mucilage trails of the diatom Navicula sp.					
31089153	3	38	theme	in	437:438	arg1	force					445:449	in situ force	437:449	in situ force mapping by AFM	437:464	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	3	39	theme	force	343:347	arg1	measurement					349:359	force measurement	343:359	force measurement	343:359	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	4	40	theme	retraction	471:480	arg1	curve					488:492	The retraction force curve	467:492	The retraction force curve	467:492	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	5	41	theme	different	642:650	arg1	energy					660:665	different surface energy	642:665	different surface energy	642:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	5	42	with	substrates	626:635	arg1	energy					660:665	different surface energy	642:665	different surface energy	642:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	4	43	theme	pattern	558:564	arg1	peak					579:582	a small saw-tooth pattern tip-mucilage peak	540:582	a small saw-tooth pattern tip-mucilage peak	540:582	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	4	44	theme	tip-mucilage	566:577	arg1	peak					579:582	a small saw-tooth pattern tip-mucilage peak	540:582	a small saw-tooth pattern tip-mucilage peak	540:582	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	0	45	theme	mucilage	30:37	arg1	Contribution					0:11	Contribution	0:11	Contribution of frustules and mucilage	0:37	Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp.					
31089153	6	46	theme	mechanical	869:878	arg1	different					921:929	different	921:929	different	921:929	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	46	theme	mechanical	869:878	arg1	properties					880:889	the mechanical properties	865:889	the mechanical properties of mucilage trails	865:908	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	5	47	theme	surface	652:658	arg1	energy					660:665	different surface energy	642:665	different surface energy	642:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	7	48	theme	symmetric	1250:1258	arg1	stretches					1260:1268	asymmetric and symmetric stretches	1235:1268	asymmetric and symmetric stretches	1235:1268	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	49	contain	had	1120:1122	arg1	both					1085:1088	both	1085:1088	both	1085:1088	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	49	contain	had	1120:1122	arg1	kinds					1101:1105	the two kinds	1093:1105	the two kinds of mucilages	1093:1118	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	49	contain	had	1120:1122	arg2	polysaccharide					1137:1150	polysaccharide	1137:1150	polysaccharide	1137:1150	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	7	49	contain	had	1120:1122	arg2	proteins					1124:1131	proteins	1124:1131	proteins	1124:1131	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	3	50	theme	mucilage	387:394	arg1	trails					396:401	the mucilage trails	383:401	the mucilage trails of live cells	383:415	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	5	51	theme	same	597:600	arg1	measurements					602:613	same measurements	597:613	same measurements on various substrates with different surface energy	597:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	6	52	theme	mucilage	894:901	arg1	trails					903:908	mucilage trails	894:908	mucilage trails	894:908	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	2	53	theme	electron	193:200	arg1	microscopy					202:211	scanning electron microscopy	184:211	scanning electron microscopy (SEM)	184:217	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	53	theme	electron	193:200	arg1	SEM					214:216	SEM	214:216	SEM	214:216	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	1	54	theme	mucilage	98:105	arg1	trails					107:112	The secreted mucilage trails	85:112	The secreted mucilage trails of the diatom Navicula sp	85:138	The secreted mucilage trails of the diatom Navicula sp.					
31089153	7	55	from	difference	1053:1062	arg1	compositions					1067:1078	compositions	1067:1078	compositions	1067:1078	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	6	56	from	addition	855:862	arg1	different					921:929	different	921:929	different	921:929	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	6	56	from	addition	855:862	arg1	properties					880:889	the mechanical properties	865:889	the mechanical properties of mucilage trails	865:908	In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length.					
31089153	8	57	theme	biofouling	1398:1407	arg1	prevention					1409:1418	biofouling prevention	1398:1418	biofouling prevention	1398:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	2	58	theme	scanning	184:191	arg1	microscopy					202:211	scanning electron microscopy	184:211	scanning electron microscopy (SEM)	184:217	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	58	theme	scanning	184:191	arg1	SEM					214:216	SEM	214:216	SEM	214:216	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	8	59	theme	motility	1385:1392	arg1	terms					1369:1373	terms	1369:1373	terms of diatom motility and biofouling prevention	1369:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	8	60	from	useful	1359:1364	arg1	terms					1369:1373	terms	1369:1373	terms of diatom motility and biofouling prevention	1369:1418	This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.					
31089153	2	61	theme	transmission	220:231	arg1	TEM					254:256	TEM	254:256	TEM	254:256	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	2	61	theme	transmission	220:231	arg1	microscope					242:251	transmission electron microscope	220:251	transmission electron microscope (TEM)	220:257	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	3	62	theme	live	406:409	arg1	cells					411:415	live cells	406:415	live cells	406:415	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	5	63	theme	various	618:624	arg1	substrates					626:635	various substrates	618:635	various substrates with different surface energy	618:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	3	64	theme	cells	411:415	arg1	trails					396:401	the mucilage trails	383:401	the mucilage trails of live cells	383:415	Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM.					
31089153	4	65	theme	tip-substrate	517:529	arg1	peak					531:534	an increased tip-substrate peak	504:534	an increased tip-substrate peak	504:534	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	5	66	theme	mucilage	685:692	arg1	medium					726:731	a medium	724:731	a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion	724:849	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	5	66	theme	mucilage	685:692	arg1	trails					694:699	the mucilage trails	681:699	the mucilage trails	681:699	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	2	67	theme	motility	159:166	arg1	process					148:154	the process	144:154	the process of motility	144:166	in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc.					
31089153	4	68	theme	saw-tooth	548:556	arg1	peak					579:582	a small saw-tooth pattern tip-mucilage peak	540:582	a small saw-tooth pattern tip-mucilage peak	540:582	The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak.					
31089153	1	69	theme	diatom	121:126	arg1	sp					137:138	the diatom Navicula sp	117:138	the diatom Navicula sp	117:138	The secreted mucilage trails of the diatom Navicula sp.					
31089153	5	70	dep	diatom	775:780	arg1	the					771:773	the	771:773	the	771:773	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
31089153	7	71	theme	other	1193:1197	arg1	components					1199:1208	some other components	1188:1208	some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches	1188:1268	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	1	72	theme	Navicula	128:135	arg1	sp					137:138	the diatom Navicula sp	117:138	the diatom Navicula sp	117:138	The secreted mucilage trails of the diatom Navicula sp.					
31089153	7	73	theme	mucilage	1161:1168	arg1	strands					1170:1176	the mucilage strands	1157:1176	the mucilage strands	1157:1176	Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, -CH2- and -CH3 asymmetric and symmetric stretches.					
31089153	5	74	from	measurements	602:613	arg1	substrates					626:635	various substrates	618:635	various substrates with different surface energy	618:665	Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom's adhesion and locomotion.					
30150145	2	0	theme	anti-tumor	444:453	arg1	mechanism					455:463	the anti-tumor mechanism	440:463	the anti-tumor mechanism of GCP	440:470	In this study, we explore the anti-tumor mechanism of GCP from the angle of gut microbiota.					
30150145	5	1	theme	HE	680:681	arg1	staining					683:690	HE staining	680:690	HE staining of tissue sections	680:709	In addition, HE staining of tissue sections reflected that GCP could effectively inhibit tumor metastasis.					
30150145	7	2	theme	GCP	1076:1078	arg1	effects					1065:1071	the anti-tumor effects	1050:1071	the anti-tumor effects of GCP and gut microbiota	1050:1097	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	6	3	theme	fecal	812:816	arg1	samples					818:824	fecal samples	812:824	fecal samples	812:824	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	3	4	dep	cells	522:526	arg1	CT-26					529:533	CT-26	529:533	CT-26	529:533	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	10	5	theme	microbiota	1424:1433	arg1	composition					1435:1445	gut microbiota composition	1420:1445	gut microbiota composition	1420:1445	These results indicate that Glycyrrhiza Polysaccharides exert anti-tumor effects by affecting gut microbiota composition.					
30150145	2	6	theme	microbiota	494:503	arg1	angle					481:485	the angle	477:485	the angle of gut microbiota	477:503	In this study, we explore the anti-tumor mechanism of GCP from the angle of gut microbiota.					
30150145	0	7	from	effect	11:16	arg1	growth					63:68	tumor growth	57:68	tumor growth through regulation of the gut microbiota composition	57:121	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	10	8	theme	gut	1420:1422	arg1	composition					1435:1445	gut microbiota composition	1420:1445	gut microbiota composition	1420:1445	These results indicate that Glycyrrhiza Polysaccharides exert anti-tumor effects by affecting gut microbiota composition.					
30150145	10	9	theme	Glycyrrhiza	1354:1364	arg1	Polysaccharides					1366:1380	Glycyrrhiza Polysaccharides	1354:1380	Glycyrrhiza Polysaccharides	1354:1380	These results indicate that Glycyrrhiza Polysaccharides exert anti-tumor effects by affecting gut microbiota composition.					
30150145	3	10	theme	mouse	572:576	arg1	model					578:582	a tumor-bearing mouse model	556:582	a tumor-bearing mouse model	556:582	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	6	11	theme	microbiota	918:927	arg1	composition					899:909	the composition	895:909	the composition of gut microbiota	895:927	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	6	12	theme	significant	835:845	arg1	change					847:852	a significant change	833:852	a significant change between the model group and GCP group in the composition of gut microbiota	833:927	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	6	13	theme	gut	914:916	arg1	microbiota					918:927	gut microbiota	914:927	gut microbiota	914:927	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	4	14	theme	tumors	632:637	arg1	weights					621:627	the weights	617:627	the weights of tumors	617:637	After 14 days of GCP treatment, the weights of tumors were significantly reduced.					
30150145	0	15	theme	gut	96:98	arg1	composition					111:121	the gut microbiota composition	92:121	the gut microbiota composition	92:121	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	9	16	theme	certain	1267:1273	arg1	extent					1275:1280	a certain extent	1265:1280	a certain extent	1265:1280	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	5	17	theme	tumor	756:760	arg1	metastasis					762:771	tumor metastasis	756:771	tumor metastasis	756:771	In addition, HE staining of tissue sections reflected that GCP could effectively inhibit tumor metastasis.					
30150145	2	18	theme	GCP	468:470	arg1	mechanism					455:463	the anti-tumor mechanism	440:463	the anti-tumor mechanism of GCP	440:470	In this study, we explore the anti-tumor mechanism of GCP from the angle of gut microbiota.					
30150145	6	19	theme	samples	818:824	arg1	sequencing					798:807	16SrRNA high-throughput sequencing	774:807	16SrRNA high-throughput sequencing of fecal samples	774:824	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	7	20	theme	anti-tumor	1054:1063	arg1	effects					1065:1071	the anti-tumor effects	1050:1071	the anti-tumor effects of GCP and gut microbiota	1050:1097	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	6	21	theme	16SrRNA	774:780	arg1	sequencing					798:807	16SrRNA high-throughput sequencing	774:807	16SrRNA high-throughput sequencing of fecal samples	774:824	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	8	22	theme	microbiota	1127:1136	arg1	depletion					1110:1118	depletion	1110:1118	depletion of gut microbiota	1110:1136	Following depletion of gut microbiota, GCP cannot inhibit tumor growth.					
30150145	1	23	theme	specific	343:350	arg1	mechanism					363:371	the specific anti-tumor mechanism	339:371	the specific anti-tumor mechanism of GCP	339:378	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	6	24	from	change	847:852	arg1	composition					899:909	the composition	895:909	the composition of gut microbiota	895:927	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	0	25	theme	Inhibition	0:9	arg1	effect					11:16	Inhibition effect	0:16	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.	0:122	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	0	26	theme	glycyrrhiza	21:31	arg1	GCP					49:51	GCP	49:51	GCP	49:51	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	0	26	theme	glycyrrhiza	21:31	arg1	polysaccharide					33:46	glycyrrhiza polysaccharide	21:46	glycyrrhiza polysaccharide (GCP)	21:52	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	8	27	theme	gut	1123:1125	arg1	microbiota					1127:1136	gut microbiota	1123:1136	gut microbiota	1123:1136	Following depletion of gut microbiota, GCP cannot inhibit tumor growth.					
30150145	0	28	theme	composition	111:121	arg1	regulation					78:87	regulation	78:87	regulation of the gut microbiota composition	78:121	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	9	29	theme	Fecal	1172:1176	arg1	experiments					1194:1204	Fecal transplantation experiments	1172:1204	Fecal transplantation experiments	1172:1204	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	3	30	theme	Colon	506:510	arg1	cells					522:526	Colon carcinoma cells	506:526	Colon carcinoma cells (CT-26)	506:534	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	1	31	theme	macromolecular	173:186	arg1	polysaccharide					188:201	as a macromolecular polysaccharide	168:201	as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM)	168:255	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	9	32	theme	tumor	1297:1301	arg1	growth					1303:1308	tumor growth	1297:1308	tumor growth	1297:1308	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	0	33	theme	microbiota	100:109	arg1	composition					111:121	the gut microbiota composition	92:121	the gut microbiota composition	92:121	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	1	34	theme	anti-tumor	352:361	arg1	mechanism					363:371	the specific anti-tumor mechanism	339:371	the specific anti-tumor mechanism of GCP	339:378	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	3	35	theme	carcinoma	512:520	arg1	cells					522:526	Colon carcinoma cells	506:526	Colon carcinoma cells (CT-26)	506:534	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	4	36	theme	treatment	606:614	arg1	days					594:597	14 days	591:597	14 days of GCP treatment	591:614	After 14 days of GCP treatment, the weights of tumors were significantly reduced.					
30150145	2	37	theme	gut	490:492	arg1	microbiota					494:503	gut microbiota	490:503	gut microbiota	490:503	In this study, we explore the anti-tumor mechanism of GCP from the angle of gut microbiota.					
30150145	9	38	theme	mice	1256:1259	arg1	feces					1235:1239	the feces	1231:1239	the feces of GCP-treated mice	1231:1259	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	0	39	theme	polysaccharide	33:46	arg1	effect					11:16	Inhibition effect	0:16	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.	0:122	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	6	40	theme	GCP	882:884	arg1	group					886:890	GCP group	882:890	GCP group	882:890	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	5	41	theme	tissue	695:700	arg1	sections					702:709	tissue sections	695:709	tissue sections	695:709	In addition, HE staining of tissue sections reflected that GCP could effectively inhibit tumor metastasis.					
30150145	3	42	theme	tumor-bearing	558:570	arg1	model					578:582	a tumor-bearing mouse model	556:582	a tumor-bearing mouse model	556:582	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	7	43	dep	GCP	1076:1078	arg1	microbiota					1088:1097	microbiota	1088:1097	microbiota	1088:1097	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	7	44	theme	gut	944:946	arg1	depletion					959:967	gut microbiota depletion and fecal transplantation experiments	944:1005	depletion	959:967	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	6	45	theme	model	866:870	arg1	group					872:876	the model group	862:876	the model group	862:876	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	5	46	theme	sections	702:709	arg1	staining					683:690	HE staining	680:690	HE staining of tissue sections	680:709	In addition, HE staining of tissue sections reflected that GCP could effectively inhibit tumor metastasis.					
30150145	7	47	theme	fecal	973:977	arg1	experiments					995:1005	gut microbiota depletion and fecal transplantation experiments	944:1005	experiments	995:1005	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	1	48	theme	GCP	376:378	arg1	mechanism					363:371	the specific anti-tumor mechanism	339:371	the specific anti-tumor mechanism of GCP	339:378	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	7	49	theme	microbiota	948:957	arg1	depletion					959:967	gut microbiota depletion and fecal transplantation experiments	944:1005	depletion	959:967	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	9	50	dep	inhibit	1289:1295	arg1	transplanting					1217:1229	transplanting	1217:1229	transplanting	1217:1229	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	1	51	theme	tumor	295:299	arg1	in vitro					308:315	tumor growth in vitro	295:315	tumor growth in vitro	295:315	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	7	52	theme	transplantation	979:993	arg1	experiments					995:1005	gut microbiota depletion and fecal transplantation experiments	944:1005	experiments	995:1005	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	8	53	theme	tumor	1158:1162	arg1	growth					1164:1169	tumor growth	1158:1169	tumor growth	1158:1169	Following depletion of gut microbiota, GCP cannot inhibit tumor growth.					
30150145	6	54	theme	high-throughput	782:796	arg1	sequencing					798:807	16SrRNA high-throughput sequencing	774:807	16SrRNA high-throughput sequencing of fecal samples	774:824	16SrRNA high-throughput sequencing of fecal samples showed a significant change between the model group and GCP group in the composition of gut microbiota.					
30150145	1	55	theme	growth	301:306	arg1	in vitro					308:315	tumor growth in vitro	295:315	tumor growth in vitro	295:315	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	3	56	used	used	541:544	arg2	cells					522:526	Colon carcinoma cells	506:526	Colon carcinoma cells (CT-26)	506:534	Colon carcinoma cells (CT-26) were used to set up a tumor-bearing mouse model.					
30150145	1	57	theme	Glycyrrhiza	124:134	arg1	Polysaccharide					146:159	Glycyrrhiza Uralensis Polysaccharide	124:159	Glycyrrhiza Uralensis Polysaccharide (GCP)	124:165	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	57	theme	Glycyrrhiza	124:134	arg1	GCP					162:164	GCP	162:164	GCP	162:164	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	58	theme	Traditional	222:232	arg1	TCM					252:254	TCM	252:254	TCM	252:254	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	58	theme	Traditional	222:232	arg1	Medicine					242:249	the Traditional Chinese Medicine	218:249	the Traditional Chinese Medicine (TCM)	218:255	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	0	59	theme	tumor	57:61	arg1	growth					63:68	tumor growth	57:68	tumor growth through regulation of the gut microbiota composition	57:121	Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.					
30150145	9	60	theme	transplantation	1178:1192	arg1	experiments					1194:1204	Fecal transplantation experiments	1172:1204	Fecal transplantation experiments	1172:1204	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	1	61	theme	Uralensis	136:144	arg1	Polysaccharide					146:159	Glycyrrhiza Uralensis Polysaccharide	124:159	Glycyrrhiza Uralensis Polysaccharide (GCP)	124:165	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	61	theme	Uralensis	136:144	arg1	GCP					162:164	GCP	162:164	GCP	162:164	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	62	theme	Chinese	234:240	arg1	TCM					252:254	TCM	252:254	TCM	252:254	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	1	62	theme	Chinese	234:240	arg1	Medicine					242:249	the Traditional Chinese Medicine	218:249	the Traditional Chinese Medicine (TCM)	218:255	Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and in vivo; however, the specific anti-tumor mechanism of GCP needs to be further investigated.					
30150145	7	63	theme	gut	1084:1086	arg1	effects					1065:1071	the anti-tumor effects	1050:1071	the anti-tumor effects of GCP and gut microbiota	1050:1097	Subsequently, gut microbiota depletion and fecal transplantation experiments further confirmed the relationship between the anti-tumor effects of GCP and gut microbiota.					
30150145	9	64	theme	GCP-treated	1244:1254	arg1	mice					1256:1259	GCP-treated mice	1244:1259	GCP-treated mice	1244:1259	Fecal transplantation experiments found that transplanting the feces of GCP-treated mice, to a certain extent, could inhibit tumor growth and metastasis.					
30150145	10	65	theme	anti-tumor	1388:1397	arg1	effects					1399:1405	anti-tumor effects	1388:1405	anti-tumor effects	1388:1405	These results indicate that Glycyrrhiza Polysaccharides exert anti-tumor effects by affecting gut microbiota composition.					
30150145	4	66	theme	GCP	602:604	arg1	treatment					606:614	GCP treatment	602:614	GCP treatment	602:614	After 14 days of GCP treatment, the weights of tumors were significantly reduced.					
30399553	3	0	theme	greater	725:731	arg1	times					719:723	three times	713:723	three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon	713:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	1	1	from	electrode	299:307	arg1	repulsion					273:281	anion repulsion	267:281	anion repulsion at the positive electrode	267:307	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	5	2	theme	improved	1140:1147	arg1	accumulation					1154:1165	improved salt accumulation	1140:1165	improved salt accumulation with polysaccharide binders	1140:1193	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	1	3	theme	adsorption	167:176	arg1	SAC					188:190	SAC	188:190	SAC	188:190	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	1	3	theme	adsorption	167:176	arg1	capacity					178:185	salt adsorption capacity	162:185	salt adsorption capacity (SAC)	162:191	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	3	4	theme	modified	854:861	arg1	carbon					863:868	chemically modified carbon	843:868	chemically modified carbon	843:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	2	5	theme	CDI	474:476	arg1	electrodes					478:487	composite CDI electrodes	464:487	composite CDI electrodes	464:487	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	5	6	dep	covered	1283:1289	arg1	membrane					1274:1281	ion-exchange membrane	1261:1281	ion-exchange membrane	1261:1281	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	5	6	dep	covered	1283:1289	arg1	MCDI					1292:1295	MCDI	1292:1295	MCDI	1292:1295	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	4	7	theme	significant	1090:1100	arg1	loss					1102:1105	a significant loss	1088:1105	a significant loss in performance	1088:1120	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	5	8	theme	CDI	1253:1255	arg1	sub-units					1298:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	2	9	theme	polysaccharides	527:541	arg1	chitosan					543:550	the ionically charged structural polysaccharides chitosan	494:550	the ionically charged structural polysaccharides chitosan	494:550	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	5	10	theme	polysaccharide	1172:1185	arg1	binders					1187:1193	polysaccharide binders	1172:1193	polysaccharide binders	1172:1193	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	4	11	theme	charge	913:918	arg1	balance					920:926	the charge balance	909:926	the charge balance in the carbon micropores	909:951	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	5	12	theme	one-dimensional	1215:1229	arg1	model					1231:1235	a one-dimensional model	1213:1235	a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1213:1306	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	6	13	theme	uncovered	1492:1500	arg1	pores					1502:1506	depleted uncovered pores	1483:1506	depleted uncovered pores	1483:1506	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	5	14	with	accumulation	1154:1165	arg1	binders					1187:1193	polysaccharide binders	1172:1193	polysaccharide binders	1172:1193	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	2	15	theme	structural	516:525	arg1	chitosan					543:550	the ionically charged structural polysaccharides chitosan	494:550	the ionically charged structural polysaccharides chitosan	494:550	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	4	16	theme	discharge	971:979	arg1	voltage					981:987	the discharge voltage	967:987	the discharge voltage of maximum adsorption	967:1009	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	2	17	theme	charged	508:514	arg1	chitosan					543:550	the ionically charged structural polysaccharides chitosan	494:550	the ionically charged structural polysaccharides chitosan	494:550	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	4	18	theme	Polysaccharide	871:884	arg1	binders					886:892	Polysaccharide binders	871:892	Polysaccharide binders	871:892	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	2	19	theme	complementary	393:405	arg1	macropores					442:451	complementary fixed charged within the electrode macropores	393:451	complementary fixed charged within the electrode macropores	393:451	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	1	20	dep	performance	134:144	arg1	measured					150:157	measured	150:157	measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS)	150:239	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	5	21	theme	ion-exchange	1261:1272	arg1	membrane					1274:1281	ion-exchange membrane	1261:1281	ion-exchange membrane	1261:1281	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	2	22	theme	composite	464:472	arg1	electrodes					478:487	composite CDI electrodes	464:487	composite CDI electrodes	464:487	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	1	23	theme	Capacitive	104:113	arg1	CDI					129:131	CDI	129:131	CDI	129:131	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	1	23	theme	Capacitive	104:113	arg1	deionization					115:126	Capacitive deionization	104:126	Capacitive deionization (CDI) performance	104:144	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	6	24	theme	depleted	1483:1490	arg1	pores					1502:1506	depleted uncovered pores	1483:1506	depleted uncovered pores	1483:1506	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	2	25	theme	co-ion	362:367	arg1	repulsion					369:377	co-ion repulsion	362:377	co-ion repulsion	362:377	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	0	26	theme	capacitive	10:19	arg1	performance					34:44	capacitive deionization performance	10:44	capacitive deionization performance	10:44	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	3	27	theme	composite	738:746	arg1	electrodes					748:757	composite electrodes	738:757	composite electrodes bound with polyvinylidene fluoride (PVDF)	738:799	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	1	28	theme	deionization	115:126	arg1	performance					134:144	Capacitive deionization (CDI) performance	104:144	Capacitive deionization (CDI) performance	104:144	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	5	29	theme	integrated	1242:1251	arg1	sub-units					1298:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	4	30	from	balance	920:926	arg1	micropores					942:951	the carbon micropores	931:951	the carbon micropores	931:951	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	6	31	theme	Model	1309:1313	arg1	simulations					1315:1325	Model simulations	1309:1325	Model simulations	1309:1325	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	6	32	theme	charged	1387:1393	arg1	polysaccharides					1395:1409	charged polysaccharides	1387:1409	charged polysaccharides	1387:1409	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	4	33	theme	cycle	1065:1069	arg1	lifetime					1071:1078	prolonged cycle lifetime	1055:1078	prolonged cycle lifetime without a significant loss in performance	1055:1120	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	1	34	theme	anion	267:271	arg1	repulsion					273:281	anion repulsion	267:281	anion repulsion at the positive electrode	267:307	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	6	35	theme	polysaccharides	1395:1409	arg1	layers					1377:1382	thin layers	1372:1382	thin layers of charged polysaccharides	1372:1409	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	6	36	theme	sequential	1436:1445	arg1	accumulation					1447:1458	a sequential accumulation	1434:1458	a sequential accumulation	1434:1458	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	1	37	theme	energy	197:202	arg1	adsorption					215:224	energy normalized adsorption	197:224	energy normalized adsorption of salt (ENAS)	197:239	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	0	38	theme	deionization	21:32	arg1	performance					34:44	capacitive deionization performance	10:44	capacitive deionization performance	10:44	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	5	39	theme	accumulation	1154:1165	arg1	mechanism					1127:1135	The mechanism	1123:1135	The mechanism of improved salt accumulation with polysaccharide binders	1123:1193	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	2	40	theme	carboxymethyl	556:568	arg1	cellulose					570:578	carboxymethyl cellulose	556:578	carboxymethyl cellulose	556:578	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	1	41	theme	normalized	204:213	arg1	adsorption					215:224	energy normalized adsorption	197:224	energy normalized adsorption of salt (ENAS)	197:239	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	0	42	theme	charged	51:57	arg1	binders					95:101	charged structural polysaccharide electrode binders	51:101	charged structural polysaccharide electrode binders	51:101	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	3	43	theme	polyvinylidene	770:783	arg1	fluoride					785:792	polyvinylidene fluoride	770:792	polyvinylidene fluoride (PVDF)	770:799	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	3	43	theme	polyvinylidene	770:783	arg1	PVDF					795:798	PVDF	795:798	PVDF	795:798	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	3	44	theme	SAC	683:685	arg1	values					696:701	SAC and ENAS values	683:701	SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon	683:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	6	45	theme	carbon	1341:1346	arg1	macropores					1348:1357	carbon macropores	1341:1357	carbon macropores covered with thin layers of charged polysaccharides	1341:1409	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	3	46	theme	CDI	816:818	arg1	electrodes					820:829	CDI electrodes	816:829	CDI electrodes composed of chemically modified carbon	816:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	0	47	theme	polysaccharide	70:83	arg1	binders					95:101	charged structural polysaccharide electrode binders	51:101	charged structural polysaccharide electrode binders	51:101	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	1	48	theme	positive	290:297	arg1	electrode					299:307	the positive electrode	286:307	the positive electrode	286:307	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	6	49	theme	thin	1372:1375	arg1	layers					1377:1382	thin layers	1372:1382	thin layers of charged polysaccharides	1372:1409	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	4	50	theme	maximum	992:998	arg1	adsorption					1000:1009	maximum adsorption	992:1009	maximum adsorption	992:1009	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	3	51	theme	charged	611:617	arg1	binders					629:635	asymmetrically charged electrode binders	596:635	asymmetrically charged electrode binders	596:635	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	0	52	theme	structural	59:68	arg1	binders					95:101	charged structural polysaccharide electrode binders	51:101	charged structural polysaccharide electrode binders	51:101	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	6	53	theme	salt	1475:1478	arg1	accumulation					1447:1458	a sequential accumulation	1434:1458	a sequential accumulation	1434:1458	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	6	53	theme	salt	1475:1478	arg1	release					1464:1470	release	1464:1470	release of salt	1464:1478	Model simulations indicate that carbon macropores covered with thin layers of charged polysaccharides increase adsorption by a sequential accumulation and release of salt to depleted uncovered pores.					
30399553	3	54	theme	electrode	619:627	arg1	binders					629:635	asymmetrically charged electrode binders	596:635	asymmetrically charged electrode binders	596:635	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	4	55	theme	carbon	935:940	arg1	micropores					942:951	the carbon micropores	931:951	the carbon micropores	931:951	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	4	56	theme	prolonged	1055:1063	arg1	lifetime					1071:1078	prolonged cycle lifetime	1055:1078	prolonged cycle lifetime without a significant loss in performance	1055:1120	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	1	57	theme	salt	229:232	arg1	adsorption					215:224	energy normalized adsorption	197:224	energy normalized adsorption of salt (ENAS)	197:239	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	1	57	theme	salt	229:232	arg1	SAC					188:190	SAC	188:190	SAC	188:190	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	1	57	theme	salt	229:232	arg1	capacity					178:185	salt adsorption capacity	162:185	salt adsorption capacity (SAC)	162:191	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	4	58	from	loss	1102:1105	arg1	performance					1110:1120	performance	1110:1120	performance	1110:1120	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	5	59	theme	covered	1283:1289	arg1	sub-units					1298:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	integrated CDI and ion-exchange membrane covered (MCDI) sub-units	1242:1306	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	4	60	theme	adsorption	1000:1009	arg1	voltage					981:987	the discharge voltage	967:987	the discharge voltage of maximum adsorption	967:1009	Polysaccharide binders did not modify the charge balance in the carbon micropores but did shift the discharge voltage of maximum adsorption, enabling a shift in operating voltage that prolonged cycle lifetime without a significant loss in performance.					
30399553	3	61	theme	ENAS	691:694	arg1	values					696:701	SAC and ENAS values	683:701	SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon	683:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	3	62	theme	co-ion	638:643	arg1	repulsion					645:653	co-ion repulsion	638:653	co-ion repulsion	638:653	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
30399553	2	63	theme	fixed	407:411	arg1	macropores					442:451	complementary fixed charged within the electrode macropores	393:451	complementary fixed charged within the electrode macropores	393:451	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	0	64	theme	electrode	85:93	arg1	binders					95:101	charged structural polysaccharide electrode binders	51:101	charged structural polysaccharide electrode binders	51:101	Enhancing capacitive deionization performance with charged structural polysaccharide electrode binders.					
30399553	5	65	theme	salt	1149:1152	arg1	accumulation					1154:1165	improved salt accumulation	1140:1165	improved salt accumulation with polysaccharide binders	1140:1193	The mechanism of improved salt accumulation with polysaccharide binders was explored with a one-dimensional model that integrated CDI and ion-exchange membrane covered (MCDI) sub-units.					
30399553	2	66	theme	charged	413:419	arg1	macropores					442:451	complementary fixed charged within the electrode macropores	393:451	complementary fixed charged within the electrode macropores	393:451	In this work, we investigate the ability to prevent co-ion repulsion by increasing complementary fixed charged within the electrode macropores by binding composite CDI electrodes with the ionically charged structural polysaccharides chitosan and carboxymethyl cellulose.					
30399553	1	67	theme	salt	162:165	arg1	SAC					188:190	SAC	188:190	SAC	188:190	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	1	67	theme	salt	162:165	arg1	capacity					178:185	salt adsorption capacity	162:185	salt adsorption capacity (SAC)	162:191	Capacitive deionization (CDI) performance, as measured by salt adsorption capacity (SAC) and energy normalized adsorption of salt (ENAS), is frequently limited by anion repulsion at the positive electrode.					
30399553	3	68	theme	similar	805:811	arg1	times					719:723	three times	713:723	three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon	713:868	When employing asymmetrically charged electrode binders, co-ion repulsion was prevented, resulting in SAC and ENAS values that were three times greater than composite electrodes bound with polyvinylidene fluoride (PVDF) and similar to CDI electrodes composed of chemically modified carbon.					
31818016	6	0	theme	cellulose-based	1331:1345	arg1	composites					1361:1370	cellulose-based electroactive composites	1331:1370	cellulose-based electroactive composites	1331:1370	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	7	1	theme	maximum	1580:1586	arg1	amplitude					1600:1608	the maximum deformation amplitude	1576:1608	the maximum deformation amplitude	1576:1608	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	7	2	theme	deformation	1427:1437	arg1	amplitude					1439:1447	the end bending deformation amplitude	1411:1447	the end bending deformation amplitude of the resulted material	1411:1472	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	7	3	theme	pure	1525:1528	arg1	film					1540:1543	the pure cellulose film	1521:1543	the pure cellulose film	1521:1543	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	0	4	theme	Composites	86:95	arg1	Preparation					13:23	the Preparation	9:23	the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	9:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	9	5	with	comparison	1804:1813	arg1	film					1839:1842	the pure cellulose film	1820:1842	the pure cellulose film	1820:1842	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	7	6	theme	higher	1501:1506	arg1	times					1495:1499	2.3 times	1491:1499	2.3 times higher than that of the pure cellulose film	1491:1543	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	7	7	theme	cellulose	1530:1538	arg1	film					1540:1543	the pure cellulose film	1521:1543	the pure cellulose film	1521:1543	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	9	8	theme	water	1849:1853	arg1	retention					1855:1863	the water retention	1845:1863	the water retention of the composite film	1845:1885	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	3	9	theme	cellulose-based	549:563	arg1	materials					579:587	cellulose-based electroactive materials	549:587	cellulose-based electroactive materials	549:587	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	1	10	theme	bionic	341:346	arg1	robots					348:353	bionic robots	341:353	bionic robots	341:353	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	8	11	theme	cellulose	1723:1731	arg1	film					1733:1736	the cellulose film	1719:1736	the cellulose film	1719:1736	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	8	12	theme	composite	1673:1681	arg1	film					1683:1686	the chitosan/cellulose composite film	1650:1686	the chitosan/cellulose composite film	1650:1686	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	1	13	theme	biological	266:275	arg1	muscles					277:283	biological muscles	266:283	biological muscles	266:283	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	14	from	prospects	317:325	arg1	aerospace					330:338	aerospace	330:338	aerospace	330:338	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	14	from	prospects	317:325	arg1	robots					348:353	bionic robots	341:353	bionic robots	341:353	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	14	from	prospects	317:325	arg1	fields					366:371	other fields	360:371	other fields	360:371	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	3	15	theme	strong	745:750	arg1	structure					764:772	the strong hydrophilic structure	741:772	the strong hydrophilic structure of cellulose itself	741:792	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	2	16	theme	cellulose-based	392:406	arg1	materials					422:430	cellulose-based electroactive materials	392:430	cellulose-based electroactive materials	392:430	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	8	17	theme	chitosan/cellulose	1654:1671	arg1	film					1683:1686	the chitosan/cellulose composite film	1650:1686	the chitosan/cellulose composite film	1650:1686	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	2	18	theme	ionic	447:451	arg1	materials					457:465	ionic EAP materials	447:465	ionic EAP materials	447:465	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	7	19	theme	end	1415:1417	arg1	amplitude					1439:1447	the end bending deformation amplitude	1411:1447	the end bending deformation amplitude of the resulted material	1411:1472	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	4	20	theme	retention	861:869	arg1	properties					871:880	good film-forming and water retention properties	833:880	good film-forming and water retention properties	833:880	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	4	21	contain	has	829:831	arg1	chitosan					820:827	chitosan	820:827	chitosan	820:827	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	4	21	contain	has	829:831	arg2	properties					871:880	good film-forming and water retention properties	833:880	good film-forming and water retention properties	833:880	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	9	22	theme	cellulose	1829:1837	arg1	film					1839:1842	the pure cellulose film	1820:1842	the pure cellulose film	1820:1842	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	7	23	theme	bending	1419:1425	arg1	amplitude					1439:1447	the end bending deformation amplitude	1411:1447	the end bending deformation amplitude of the resulted material	1411:1472	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	2	24	theme	higher	487:492	arg1	modulus					502:508	higher Young's modulus	487:508	higher Young's modulus	487:508	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	1	25	theme	Electro-actuated	98:113	arg1	polymer					115:121	Electro-actuated polymer	98:121	Electro-actuated polymer (EAP)	98:127	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	25	theme	Electro-actuated	98:113	arg1	EAP					124:126	EAP	124:126	EAP	124:126	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	8	26	theme	film	1683:1686	arg1	strength					1638:1645	The tensile strength	1626:1645	The tensile strength of the chitosan/cellulose composite film	1626:1686	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	8	26	theme	film	1683:1686	arg1	higher					1699:1704	higher	1699:1704	higher	1699:1704	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	5	27	dep	[EMIM	1052:1056	arg1	Ac					1058:1059	Ac	1058:1059	Ac	1058:1059	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	6	28	theme	resulted	1292:1299	arg1	composite					1301:1309	the resulted composite	1288:1309	the resulted composite	1288:1309	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	9	29	from	changes	1999:2005	arg1	humidity					2024:2031	environmental humidity	2010:2031	environmental humidity	2010:2031	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	7	30	theme	material	1465:1472	arg1	amplitude					1439:1447	the end bending deformation amplitude	1411:1447	the end bending deformation amplitude of the resulted material	1411:1472	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	3	31	theme	electroactive	565:577	arg1	materials					579:587	cellulose-based electroactive materials	549:587	cellulose-based electroactive materials	549:587	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	6	32	theme	composite	1301:1309	arg1	surface					1277:1283	the surface	1273:1283	the surface of the resulted composite	1273:1309	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	3	33	theme	cellulose	777:785	arg1	structure					764:772	the strong hydrophilic structure	741:772	the strong hydrophilic structure of cellulose itself	741:792	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	7	34	theme	deformation	1588:1598	arg1	amplitude					1600:1608	the maximum deformation amplitude	1576:1608	the maximum deformation amplitude	1576:1608	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	1	35	contain	has	296:298	arg2	prospects					317:325	good application prospects	300:325	good application prospects in aerospace, bionic robots, and other fields	300:371	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	35	contain	has	296:298	arg1	it					293:294	it	293:294	it	293:294	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	1	36	theme	external	183:190	arg1	field					201:205	an external electric field	180:205	an external electric field	180:205	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	2	37	theme	materials	422:430	arg1	similar					436:442	similar	436:442	similar	436:442	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	2	37	theme	materials	422:430	arg1	properties					378:387	The properties	374:387	The properties of cellulose-based electroactive materials	374:430	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	3	38	theme	hydrophilic	752:762	arg1	structure					764:772	the strong hydrophilic structure	741:772	the strong hydrophilic structure of cellulose itself	741:792	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	5	39	theme	chitosan/cellulose	956:973	arg1	film					985:988	a chitosan/cellulose composite film	954:988	a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac),	954:1061	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	1	40	theme	other	360:364	arg1	fields					366:371	other fields	360:371	other fields	360:371	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	8	41	theme	tensile	1630:1636	arg1	strength					1638:1645	The tensile strength	1626:1645	The tensile strength of the chitosan/cellulose composite film	1626:1686	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	8	41	theme	tensile	1630:1636	arg1	higher					1699:1704	higher	1699:1704	higher	1699:1704	The tensile strength of the chitosan/cellulose composite film was 53.68% higher than that of the cellulose film, and the Young's modulus was increased by 72.52%.					
31818016	1	42	theme	electric	192:199	arg1	field					201:205	an external electric field	180:205	an external electric field	180:205	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	0	43	theme	Ionic	28:32	arg1	Composites					86:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	6	44	theme	composites	1361:1370	arg1	kind					1323:1326	a kind	1321:1326	a kind of cellulose-based electroactive composites	1321:1370	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	2	45	theme	energy	520:525	arg1	consumption					527:537	lower energy consumption	514:537	lower energy consumption	514:537	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	6	46	dep	poly	1184:1187	arg1	styrene					1223:1229	styrene	1223:1229	styrene sulfonate	1223:1239	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	4	47	theme	film-forming	838:849	arg1	properties					871:880	good film-forming and water retention properties	833:880	good film-forming and water retention properties	833:880	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	1	48	theme	field	201:205	arg1	action					170:175	the action	166:175	the action of an external electric field	166:205	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	6	49	theme	electroactive	1347:1359	arg1	composites					1361:1370	cellulose-based electroactive composites	1331:1370	cellulose-based electroactive composites	1331:1370	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	2	50	theme	lower	514:518	arg1	consumption					527:537	lower energy consumption	514:537	lower energy consumption	514:537	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	5	51	theme	regeneration	1098:1109	arg1	process					1111:1117	regeneration process	1098:1117	regeneration process	1098:1117	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	4	52	theme	good	833:836	arg1	properties					871:880	good film-forming and water retention properties	833:880	good film-forming and water retention properties	833:880	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	9	53	theme	composite	1872:1880	arg1	film					1882:1885	the composite film	1868:1885	the composite film	1868:1885	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	2	54	theme	Young	494:498	arg1	modulus					502:508	higher Young's modulus	487:508	higher Young's modulus	487:508	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	2	55	theme	EAP	453:455	arg1	materials					457:465	ionic EAP materials	447:465	ionic EAP materials	447:465	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	7	56	theme	same	1555:1558	arg1	conditions					1560:1569	the same conditions	1551:1569	the same conditions	1551:1569	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	0	57	theme	Chitosan/Cellulose-Based	47:70	arg1	Composites					86:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	4	58	theme	water	855:859	arg1	retention					861:869	water retention	855:869	water retention	855:869	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	9	59	theme	film	1882:1885	arg1	retention					1855:1863	the water retention	1845:1863	the water retention of the composite film	1845:1885	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	0	60	theme	Liquid	34:39	arg1	Composites					86:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	1	61	theme	good	300:303	arg1	prospects					317:325	good application prospects	300:325	good application prospects in aerospace, bionic robots, and other fields	300:371	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	6	62	dep	PEDOT	1243:1247	arg1	PSS					1250:1252	PSS	1250:1252	PEDOT: PSS	1243:1252	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	3	63	theme	ambient	688:694	arg1	humidity					696:703	ambient humidity	688:703	ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself	688:792	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	2	64	contain	have	482:485	arg2	modulus					502:508	higher Young's modulus	487:508	higher Young's modulus	487:508	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	2	64	contain	have	482:485	arg1	they					477:480	they	477:480	they	477:480	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	2	64	contain	have	482:485	arg2	consumption					527:537	lower energy consumption	514:537	lower energy consumption	514:537	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	9	65	theme	material	1987:1994	arg1	resistance					1969:1978	the resistance	1965:1978	the resistance of the material to changes in environmental humidity	1965:2031	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	1	66	theme	application	305:315	arg1	prospects					317:325	good application prospects	300:325	good application prospects in aerospace, bionic robots, and other fields	300:371	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	3	67	theme	actuation	626:634	arg1	performance					636:646	a more obvious deficiency-their actuation performance	594:646	a more obvious deficiency-their actuation performance	594:646	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	5	68	theme	composite	975:983	arg1	film					985:988	a chitosan/cellulose composite film	954:988	a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac),	954:1061	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	6	69	theme	conductive	1164:1173	arg1	polymer					1175:1181	a conductive polymer	1162:1181	a conductive polymer	1162:1181	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	6	69	theme	conductive	1164:1173	arg1	poly					1184:1187	poly	1184:1187	poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS)	1184:1253	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	9	70	theme	environmental	2010:2022	arg1	humidity					2024:2031	environmental humidity	2010:2031	environmental humidity	2010:2031	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	5	71	theme	1-ethyl-3-methylimidazolium	1015:1041	arg1	acetate					1043:1049	1-ethyl-3-methylimidazolium acetate	1015:1049	1-ethyl-3-methylimidazolium acetate ([EMIM]Ac)	1015:1060	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	5	71	theme	1-ethyl-3-methylimidazolium	1015:1041	arg1	[EMIM					1052:1056	[EMIM	1052:1056	[EMIM	1052:1056	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	5	71	theme	1-ethyl-3-methylimidazolium	1015:1041	arg1	liquid					1007:1012	liquid	1007:1012	liquid	1007:1012	In this study, a chitosan/cellulose composite film doped with ionic liquid, 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac), was prepared by co-dissolution and regeneration process using [EMIM]Ac as the solvent.					
31818016	4	72	with	compatibility	891:903	arg1	cellulose					910:918	cellulose	910:918	cellulose	910:918	Compared with cellulose, chitosan has good film-forming and water retention properties, and its compatibility with cellulose is very excellent.					
31818016	1	73	theme	similar	217:223	arg1	characteristics					236:250	similar behavioral characteristics	217:250	similar behavioral characteristics	217:250	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	0	74	theme	Electroactive	72:84	arg1	Composites					86:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	9	75	theme	pure	1824:1827	arg1	film					1839:1842	the pure cellulose film	1820:1842	the pure cellulose film	1820:1842	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	3	76	theme	obvious	601:607	arg1	performance					636:646	a more obvious deficiency-their actuation performance	594:646	a more obvious deficiency-their actuation performance	594:646	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	2	77	theme	electroactive	408:420	arg1	materials					422:430	cellulose-based electroactive materials	392:430	cellulose-based electroactive materials	392:430	The properties of cellulose-based electroactive materials are similar to ionic EAP materials, although they have higher Young's modulus and lower energy consumption.					
31818016	6	78	dep	styrene	1223:1229	arg1	sulfonate					1231:1239	sulfonate	1231:1239	styrene sulfonate	1223:1239	After that, a conductive polymer, poly(3,4-ethylenedioxythiophene)/poly (styrene sulfonate) (PEDOT: PSS), was deposited on the surface of the resulted composite, and then a kind of cellulose-based electroactive composites were obtained.					
31818016	1	79	theme	behavioral	225:234	arg1	characteristics					236:250	similar behavioral characteristics	217:250	similar behavioral characteristics	217:250	Electro-actuated polymer (EAP) can change its shape or volume under the action of an external electric field and shows similar behavioral characteristics with those of biological muscles, and so it has good application prospects in aerospace, bionic robots, and other fields.					
31818016	0	80	theme	Doped	41:45	arg1	Composites					86:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites	28:95	Study on the Preparation of Ionic Liquid Doped Chitosan/Cellulose-Based Electroactive Composites.					
31818016	7	81	theme	resulted	1456:1463	arg1	material					1465:1472	the resulted material	1452:1472	the resulted material	1452:1472	The results showed that the end bending deformation amplitude of the resulted material was increased by 2.3 times higher than that of the pure cellulose film under the same conditions, and the maximum deformation amplitude reached 7.3 mm.					
31818016	3	82	theme	deficiency-their	609:624	arg1	performance					636:646	a more obvious deficiency-their actuation performance	594:646	a more obvious deficiency-their actuation performance	594:646	However, cellulose-based electroactive materials have a more obvious deficiency-their actuation performance is often more significantly affected by ambient humidity due to the hygroscopicity caused by the strong hydrophilic structure of cellulose itself.					
31818016	9	83	theme	absorption	1911:1920	arg1	rate					1922:1925	the water absorption rate	1901:1925	the water absorption rate	1901:1925	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
31818016	9	84	theme	water	1905:1909	arg1	rate					1922:1925	the water absorption rate	1901:1925	the water absorption rate	1901:1925	Furthermore, in comparison with the pure cellulose film, the water retention of the composite film increased and the water absorption rate decreased obviously, which meant that the resistance of the material to changes in environmental humidity was greatly improved.					
30318233	0	0	theme	species	102:108	arg1	production					110:119	reactive oxygen species production	86:119	reactive oxygen species production in in vitro and in zebrafish model	86:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	8	1	theme	SP	942:943	arg1	embryos					963:969	SP treated zebrafish embryos	942:969	SP treated zebrafish embryos	942:969	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
30318233	6	2	theme	scavenging	614:623	arg1	activity					625:632	ABTS radical scavenging activity	601:632	ABTS radical scavenging activity	601:632	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	1	3	theme	sulfated	161:168	arg1	SP					186:187	SP	186:187	SP	186:187	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	1	3	theme	sulfated	161:168	arg1	polysaccharide					170:183	The sulfated polysaccharide	157:183	The sulfated polysaccharide (SP)	157:188	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	7	4	theme	mediated	783:790	arg1	stress					802:807	H2O2 mediated oxidative stress	778:807	H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS	778:864	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	0	5	theme	oxygen	95:100	arg1	species					102:108	reactive oxygen species	86:108	reactive oxygen species production in in vitro and in zebrafish model	86:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	3	6	theme	nitrogen	440:447	arg1	nitrogen					440:447	nitrogen	440:447	nitrogen	440:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	6	theme	nitrogen	440:447	arg1	%					396:396	28.8%	392:396	28.8% of carbon	392:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	6	theme	nitrogen	440:447	arg1	hydrogen					418:425	hydrogen	418:425	hydrogen	418:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	6	theme	nitrogen	440:447	arg1	carbon					401:406	carbon	401:406	carbon	401:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	6	theme	nitrogen	440:447	arg1	%					435:435	0.29%	431:435	0.29% of nitrogen	431:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	6	theme	nitrogen	440:447	arg1	%					413:413	4.02%	409:413	4.02% of hydrogen	409:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	7	7	theme	intracellular	848:860	arg1	ROS					862:864	scavenging intracellular ROS	837:864	scavenging intracellular ROS	837:864	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	8	8	theme	zebrafish	953:961	arg1	embryos					963:969	SP treated zebrafish embryos	942:969	SP treated zebrafish embryos	942:969	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
30318233	3	9	theme	Elemental	361:369	arg1	analysis					371:378	Elemental analysis	361:378	Elemental analysis of SP	361:384	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	8	10	theme	treated	945:951	arg1	embryos					963:969	SP treated zebrafish embryos	942:969	SP treated zebrafish embryos	942:969	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
30318233	8	11	theme	cell	899:902	arg1	death					904:908	cell death	899:908	cell death	899:908	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
30318233	6	12	theme	radical	606:612	arg1	activity					625:632	ABTS radical scavenging activity	601:632	ABTS radical scavenging activity	601:632	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	9	13	theme	pharmacological	1100:1114	arg1	industries					1116:1125	pharmacological industries	1100:1125	pharmacological industries	1100:1125	The protective effect of SP against oxidative stress might be utilized in pharmacological industries.					
30318233	6	14	theme	ABTS	601:604	arg1	activity					625:632	ABTS radical scavenging activity	601:632	ABTS radical scavenging activity	601:632	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	7	15	theme	scavenging	837:846	arg1	ROS					862:864	scavenging intracellular ROS	837:864	scavenging intracellular ROS	837:864	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	2	16	theme	SP	266:267	arg1	composition					251:261	The chemical composition	238:261	The chemical composition of SP	238:267	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	3	17	theme	carbon	401:406	arg1	nitrogen					440:447	nitrogen	440:447	nitrogen	440:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	17	theme	carbon	401:406	arg1	%					396:396	28.8%	392:396	28.8% of carbon	392:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	17	theme	carbon	401:406	arg1	hydrogen					418:425	hydrogen	418:425	hydrogen	418:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	17	theme	carbon	401:406	arg1	carbon					401:406	carbon	401:406	carbon	401:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	17	theme	carbon	401:406	arg1	%					435:435	0.29%	431:435	0.29% of nitrogen	431:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	17	theme	carbon	401:406	arg1	%					413:413	4.02%	409:413	4.02% of hydrogen	409:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	0	18	from	tenerrimum	39:48	arg1	polysaccharide					9:22	Sulfated polysaccharide	0:22	Sulfated polysaccharide from Sargassum tenerrimum	0:48	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	6	19	dep	DPPH	592:595	arg1	The					588:590	The	588:590	The	588:590	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	2	20	theme	sugar	301:305	arg1	protein					324:330	protein	324:330	protein	324:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	20	theme	sugar	301:305	arg1	sugar					301:305	total sugar	295:305	total sugar	295:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	20	theme	sugar	301:305	arg1	%					347:347	25.6 ± 0.45%	336:347	25.6 ± 0.45% of sulfate	336:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	20	theme	sugar	301:305	arg1	%					319:319	1.14 ± 0.28%	308:319	1.14 ± 0.28% of protein	308:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	20	theme	sugar	301:305	arg1	sulfate					352:358	sulfate	352:358	sulfate	352:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	20	theme	sugar	301:305	arg1	%					290:290	57 ± 0.29%	281:290	57 ± 0.29% of total sugar	281:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	protein					324:330	protein	324:330	protein	324:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	sugar					301:305	total sugar	295:305	total sugar	295:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	%					347:347	25.6 ± 0.45%	336:347	25.6 ± 0.45% of sulfate	336:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	%					319:319	1.14 ± 0.28%	308:319	1.14 ± 0.28% of protein	308:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	sulfate					352:358	sulfate	352:358	sulfate	352:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	21	theme	sulfate	352:358	arg1	%					290:290	57 ± 0.29%	281:290	57 ± 0.29% of total sugar	281:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	0	22	theme	Sulfated	0:7	arg1	polysaccharide					9:22	Sulfated polysaccharide	0:22	Sulfated polysaccharide from Sargassum tenerrimum	0:48	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	8	23	theme	ROS	880:882	arg1	generation					884:893	ROS generation	880:893	ROS generation	880:893	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
30318233	2	24	theme	total	295:299	arg1	sugar					301:305	total sugar	295:305	total sugar	295:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	9	25	used	utilized	1088:1095	arg2	effect					1041:1046	The protective effect	1026:1046	The protective effect of SP against oxidative stress	1026:1077	The protective effect of SP against oxidative stress might be utilized in pharmacological industries.					
30318233	7	26	from	stress	802:807	arg1	cells					823:827	fibroblast cells	812:827	fibroblast cells	812:827	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	2	27	theme	protein	324:330	arg1	protein					324:330	protein	324:330	protein	324:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	27	theme	protein	324:330	arg1	sugar					301:305	total sugar	295:305	total sugar	295:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	27	theme	protein	324:330	arg1	%					347:347	25.6 ± 0.45%	336:347	25.6 ± 0.45% of sulfate	336:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	27	theme	protein	324:330	arg1	%					319:319	1.14 ± 0.28%	308:319	1.14 ± 0.28% of protein	308:330	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	27	theme	protein	324:330	arg1	sulfate					352:358	sulfate	352:358	sulfate	352:358	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	2	27	theme	protein	324:330	arg1	%					290:290	57 ± 0.29%	281:290	57 ± 0.29% of total sugar	281:305	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	3	28	theme	SP	383:384	arg1	analysis					371:378	Elemental analysis	361:378	Elemental analysis of SP	361:384	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	7	29	theme	protective	754:763	arg1	role					765:768	a protective role	752:768	a protective role	752:768	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	0	30	theme	zebrafish	140:148	arg1	model					150:154	zebrafish model	140:154	zebrafish model	140:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	9	31	theme	protective	1030:1039	arg1	effect					1041:1046	The protective effect	1026:1046	The protective effect of SP against oxidative stress	1026:1077	The protective effect of SP against oxidative stress might be utilized in pharmacological industries.					
30318233	1	32	theme	brown	212:216	arg1	tenerrimum					226:235	the brown alga S. tenerrimum	208:235	the brown alga S. tenerrimum	208:235	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	4	33	theme	molecular	454:462	arg1	weight					464:469	The molecular weight	450:469	The molecular weight of SP	450:475	The molecular weight of SP was estimated as 31 kDa.					
30318233	4	33	theme	molecular	454:462	arg1	31 kDa					494:499	31 kDa	494:499	31 kDa	494:499	The molecular weight of SP was estimated as 31 kDa.					
30318233	5	34	theme	TGA	574:576	arg1	analysis					578:585	TGA analysis	574:585	TGA analysis	574:585	Further, the SP was characterized through FT-IR, 1H-NMR, GC-MS, XRD and TGA analysis.					
30318233	0	35	theme	oxidative	61:69	arg1	stress					71:76	oxidative stress	61:76	oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model	61:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	4	36	theme	SP	474:475	arg1	weight					464:469	The molecular weight	450:469	The molecular weight of SP	450:475	The molecular weight of SP was estimated as 31 kDa.					
30318233	4	36	theme	SP	474:475	arg1	31 kDa					494:499	31 kDa	494:499	31 kDa	494:499	The molecular weight of SP was estimated as 31 kDa.					
30318233	1	37	attach	isolated	194:201	arg2	polysaccharide					170:183	The sulfated polysaccharide	157:183	The sulfated polysaccharide (SP)	157:188	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	1	37	attach	isolated	194:201	arg1	tenerrimum					226:235	the brown alga S. tenerrimum	208:235	the brown alga S. tenerrimum	208:235	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	1	37	attach	isolated	194:201	arg2	SP					186:187	SP	186:187	SP	186:187	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	6	38	theme	25-125 μg/mL	701:712	arg1	concentration					684:696	the concentration	680:696	the concentration of 25-125 μg/mL	680:712	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	3	39	theme	hydrogen	418:425	arg1	nitrogen					440:447	nitrogen	440:447	nitrogen	440:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	39	theme	hydrogen	418:425	arg1	%					396:396	28.8%	392:396	28.8% of carbon	392:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	39	theme	hydrogen	418:425	arg1	hydrogen					418:425	hydrogen	418:425	hydrogen	418:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	39	theme	hydrogen	418:425	arg1	carbon					401:406	carbon	401:406	carbon	401:406	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	39	theme	hydrogen	418:425	arg1	%					435:435	0.29%	431:435	0.29% of nitrogen	431:447	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	3	39	theme	hydrogen	418:425	arg1	%					413:413	4.02%	409:413	4.02% of hydrogen	409:425	Elemental analysis of SP shows 28.8% of carbon, 4.02% of hydrogen and 0.29% of nitrogen.					
30318233	7	40	theme	fibroblast	812:821	arg1	cells					823:827	fibroblast cells	812:827	fibroblast cells	812:827	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	1	41	theme	alga	218:221	arg1	tenerrimum					226:235	the brown alga S. tenerrimum	208:235	the brown alga S. tenerrimum	208:235	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	9	42	theme	SP	1051:1052	arg1	effect					1041:1046	The protective effect	1026:1046	The protective effect of SP against oxidative stress	1026:1077	The protective effect of SP against oxidative stress might be utilized in pharmacological industries.					
30318233	6	43	theme	SP	637:638	arg1	activity					625:632	ABTS radical scavenging activity	601:632	ABTS radical scavenging activity	601:632	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	6	43	theme	SP	637:638	arg1	DPPH					592:595	DPPH	592:595	DPPH	592:595	The DPPH and ABTS radical scavenging activity of SP showed 34.03-62.70% and 22.94-38.04% at the concentration of 25-125 μg/mL respectively.					
30318233	7	44	theme	oxidative	792:800	arg1	stress					802:807	H2O2 mediated oxidative stress	778:807	H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS	778:864	In addition, SP exerted a protective role against H2O2 mediated oxidative stress in fibroblast cells through scavenging intracellular ROS.					
30318233	1	45	theme	S.	223:224	arg1	tenerrimum					226:235	the brown alga S. tenerrimum	208:235	the brown alga S. tenerrimum	208:235	The sulfated polysaccharide (SP) was isolated from the brown alga S. tenerrimum.					
30318233	0	46	theme	reactive	86:93	arg1	species					102:108	reactive oxygen species	86:108	reactive oxygen species production in in vitro and in zebrafish model	86:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	0	47	from	production	110:119	arg1	model					150:154	zebrafish model	140:154	zebrafish model	140:154	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	0	47	from	production	110:119	arg1	vitro					127:131	in vitro	124:131	in vitro	124:131	Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model.					
30318233	2	48	theme	chemical	242:249	arg1	composition					251:261	The chemical composition	238:261	The chemical composition of SP	238:267	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	9	49	theme	oxidative	1062:1070	arg1	stress					1072:1077	oxidative stress	1062:1077	oxidative stress	1062:1077	The protective effect of SP against oxidative stress might be utilized in pharmacological industries.					
30318233	2	50	dep	composed	269:276	arg1	of					278:279	of	278:279	of	278:279	The chemical composition of SP composed of 57 ± 0.29% of total sugar, 1.14 ± 0.28% of protein and 25.6 ± 0.45% of sulfate.					
30318233	8	51	theme	survival	997:1004	arg1	rate					1006:1009	the survival rate	993:1009	the survival rate	993:1009	Furthermore, ROS generation and cell death were significantly decreased in SP treated zebrafish embryos at 150 μg/mL, whereas the survival rate was increased.					
29337101	1	0	theme	exopolysaccharide	161:177	arg1	potentials					241:250	the in-vitro probiotic potentials	218:250	the in-vitro probiotic potentials of this strain	218:265	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	0	theme	exopolysaccharide	161:177	arg1	optimization					123:134	optimization	123:134	optimization	123:134	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	0	theme	exopolysaccharide	161:177	arg1	characterization					141:156	characterization	141:156	characterization	141:156	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	0	theme	exopolysaccharide	161:177	arg1	production					111:120	production	111:120	production	111:120	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	9	1	theme	Central	892:898	arg1	CCD					918:920	CCD	918:920	CCD	918:920	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	9	1	theme	Central	892:898	arg1	Design					910:915	Central Composite Design	892:915	Central Composite Design (CCD)	892:921	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	9	2	theme	Response	927:934	arg1	Methodology					944:954	Response Surface Methodology	927:954	Response Surface Methodology (RSM)	927:960	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	9	2	theme	Response	927:934	arg1	RSM					957:959	RSM	957:959	RSM	957:959	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	6	3	theme	hydroxyl	650:657	arg1	presence					638:645	the presence	634:645	the presence of hydroxyl, carboxyl, N-acetyl and amine groups	634:694	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
29337101	1	4	theme	strain	260:265	arg1	potentials					241:250	the in-vitro probiotic potentials	218:250	the in-vitro probiotic potentials of this strain	218:265	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	4	theme	strain	260:265	arg1	optimization					123:134	optimization	123:134	optimization	123:134	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	4	theme	strain	260:265	arg1	characterization					141:156	characterization	141:156	characterization	141:156	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	4	theme	strain	260:265	arg1	production					111:120	production	111:120	production	111:120	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	9	5	theme	Surface	936:942	arg1	Methodology					944:954	Response Surface Methodology	927:954	Response Surface Methodology (RSM)	927:960	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	9	5	theme	Surface	936:942	arg1	RSM					957:959	RSM	957:959	RSM	957:959	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	10	6	theme	sucrose	1048:1054	arg1	24.00 g/L					1071:1079	24.00 g/L	1071:1079	24.00 g/L	1071:1079	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	6	theme	sucrose	1048:1054	arg1	concentration					1056:1068	sucrose concentration	1048:1068	sucrose concentration (24.00 g/L)	1048:1080	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	4	7	theme	homopolysaccharide	489:506	arg1	nature					508:513	its homopolysaccharide nature	485:513	its homopolysaccharide nature	485:513	HPLC analysis revealed the presence of glucose monomers, indicating its homopolysaccharide nature.					
29337101	1	8	from	characterization	141:156	arg1	confusa					200:206	confusa	200:206	confusa	200:206	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	3	9	theme	purified	355:362	arg1	EPS					364:366	The purified EPS	351:366	The purified EPS	351:366	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	12	10	theme	salts	1337:1341	arg1	presence					1320:1327	the presence	1316:1327	the presence of bile salts (0.50% (w/v)) for 4 h	1316:1363	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	4	11	theme	HPLC	417:420	arg1	analysis					422:429	HPLC analysis	417:429	HPLC analysis	417:429	HPLC analysis revealed the presence of glucose monomers, indicating its homopolysaccharide nature.					
29337101	12	12	theme	bile	1332:1335	arg1	salts					1337:1341	bile salts	1332:1341	bile salts (0.50% (w/v))	1332:1355	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	5	13	theme	structural	520:529	arg1	characteristics					531:545	The structural characteristics	516:545	The structural characteristics of the EPS	516:556	The structural characteristics of the EPS were investigated by FTIR, and NMR spectroscopy.					
29337101	12	14	contain	possess	1234:1240	arg1	strain					1214:1219	This strain	1209:1219	This strain	1209:1219	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	12	14	contain	possess	1234:1240	arg2	attributes					1262:1271	desirable probiotic attributes	1242:1271	desirable probiotic attributes	1242:1271	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	5	15	theme	NMR	589:591	arg1	spectroscopy					593:604	FTIR, and NMR spectroscopy	579:604	spectroscopy	593:604	The structural characteristics of the EPS were investigated by FTIR, and NMR spectroscopy.					
29337101	13	16	theme	useful	1487:1492	arg1	applications					1494:1505	useful applications	1487:1505	useful applications in industries	1487:1519	The results obtained from this study demonstrate W. confusa OF126 as a promising probiotic and the EPS produced can find useful applications in industries.					
29337101	0	17	from	confusa	44:50	arg1	polysaccharide					14:27	Extracellular polysaccharide	0:27	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.	0:105	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.					
29337101	0	18	theme	Extracellular	0:12	arg1	polysaccharide					14:27	Extracellular polysaccharide	0:27	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.	0:105	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.					
29337101	10	19	theme	yeast	1097:1101	arg1	extract					1103:1109	yeast extract	1097:1109	yeast extract (2.50%)	1097:1117	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	19	theme	yeast	1097:1101	arg1	%					1116:1116	2.50%	1112:1116	2.50%	1112:1116	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	9	20	theme	significant	851:861	arg1	factors					863:869	Four significant factors	846:869	Four significant factors	846:869	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	2	21	theme	modified-MRS	313:324	arg1	broth					326:330	sucrose modified-MRS broth	305:330	sucrose modified-MRS broth	305:330	The EPS produced on sucrose modified-MRS broth was characterized.					
29337101	10	22	theme	cultivation	1020:1030	arg1	conditions					985:994	The predicted optimum conditions	963:994	The predicted optimum conditions for EPS production	963:1013	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	22	theme	cultivation	1020:1030	arg1	time					1032:1035	cultivation time	1020:1035	cultivation time (48.50 h)	1020:1045	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	22	theme	cultivation	1020:1030	arg1	48.50 h					1038:1044	48.50 h	1038:1044	48.50 h	1038:1044	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	3	23	theme	average	375:381	arg1	weight					393:398	an average molecular weight	372:398	an average molecular weight of 1.1 × 106 Da	372:414	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	1	24	from	optimization	123:134	arg1	confusa					200:206	confusa	200:206	confusa	200:206	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	2	25	theme	sucrose	305:311	arg1	broth					326:330	sucrose modified-MRS broth	305:330	sucrose modified-MRS broth	305:330	The EPS produced on sucrose modified-MRS broth was characterized.					
29337101	3	26	theme	molecular	383:391	arg1	weight					393:398	an average molecular weight	372:398	an average molecular weight of 1.1 × 106 Da	372:414	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	4	27	theme	monomers	464:471	arg1	presence					444:451	the presence	440:451	the presence of glucose monomers	440:471	HPLC analysis revealed the presence of glucose monomers, indicating its homopolysaccharide nature.					
29337101	6	28	theme	FTIR	607:610	arg1	spectroscopy					612:623	FTIR spectroscopy	607:623	FTIR spectroscopy	607:623	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
29337101	12	29	dep	salts	1337:1341	arg1	w/v					1351:1353	w/v	1351:1353	w/v	1351:1353	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	12	29	dep	salts	1337:1341	arg1	%					1348:1348	0.50%	1344:1348	0.50% (w/v)	1344:1354	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	4	30	theme	glucose	456:462	arg1	monomers					464:471	glucose monomers	456:471	glucose monomers	456:471	HPLC analysis revealed the presence of glucose monomers, indicating its homopolysaccharide nature.					
29337101	10	31	theme	predicted	967:975	arg1	conditions					985:994	The predicted optimum conditions	963:994	The predicted optimum conditions for EPS production	963:1013	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	31	theme	predicted	967:975	arg1	time					1032:1035	cultivation time	1020:1035	cultivation time (48.50 h)	1020:1045	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	6	32	theme	groups	689:694	arg1	presence					638:645	the presence	634:645	the presence of hydroxyl, carboxyl, N-acetyl and amine groups	634:694	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
29337101	8	33	theme	strong	808:813	arg1	activity					836:843	strong in-vitro antioxidant activity	808:843	strong in-vitro antioxidant activity	808:843	The EPS showed strong in-vitro antioxidant activity.					
29337101	7	34	contain	contained	733:741	arg2	linkage					753:759	α-(1 → 6) linkage	743:759	α-(1 → 6) linkage	743:759	NMR analysis confirmed that the EPS contained α-(1 → 6) linkage and α-(1 → 3) branched linkage.					
29337101	7	34	contain	contained	733:741	arg1	EPS					729:731	the EPS	725:731	the EPS	725:731	NMR analysis confirmed that the EPS contained α-(1 → 6) linkage and α-(1 → 3) branched linkage.					
29337101	3	35	theme	1.1 × 106 Da	403:414	arg1	weight					393:398	an average molecular weight	372:398	an average molecular weight of 1.1 × 106 Da	372:414	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	1	36	from	potentials	241:250	arg1	confusa					200:206	confusa	200:206	confusa	200:206	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	8	37	theme	antioxidant	824:834	arg1	activity					836:843	strong in-vitro antioxidant activity	808:843	strong in-vitro antioxidant activity	808:843	The EPS showed strong in-vitro antioxidant activity.					
29337101	9	38	theme	Composite	900:908	arg1	CCD					918:920	CCD	918:920	CCD	918:920	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	9	38	theme	Composite	900:908	arg1	Design					910:915	Central Composite Design	892:915	Central Composite Design (CCD)	892:921	Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).					
29337101	7	39	theme	NMR	697:699	arg1	analysis					701:708	NMR analysis	697:708	NMR analysis	697:708	NMR analysis confirmed that the EPS contained α-(1 → 6) linkage and α-(1 → 3) branched linkage.					
29337101	1	40	from	confusa	200:206	arg1	characterization					141:156	characterization	141:156	characterization	141:156	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	40	from	confusa	200:206	arg1	production					111:120	production	111:120	production	111:120	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	40	from	confusa	200:206	arg1	potentials					241:250	the in-vitro probiotic potentials	218:250	the in-vitro probiotic potentials of this strain	218:265	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	40	from	confusa	200:206	arg1	exopolysaccharide					161:177	exopolysaccharide	161:177	exopolysaccharide (EPS) from Weissella confusa OF126	161:212	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	40	from	confusa	200:206	arg1	optimization					123:134	optimization	123:134	optimization	123:134	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	40	from	confusa	200:206	arg1	EPS					180:182	EPS	180:182	EPS	180:182	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	6	41	theme	amine	683:687	arg1	groups					689:694	amine groups	683:694	amine groups	683:694	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
29337101	12	42	theme	probiotic	1252:1260	arg1	attributes					1262:1271	desirable probiotic attributes	1242:1271	desirable probiotic attributes	1242:1271	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	10	43	theme	EPS	1000:1002	arg1	production					1004:1013	EPS production	1000:1013	EPS production	1000:1013	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	3	44	contain	had	368:370	arg1	EPS					364:366	The purified EPS	351:366	The purified EPS	351:366	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	3	44	contain	had	368:370	arg2	weight					393:398	an average molecular weight	372:398	an average molecular weight of 1.1 × 106 Da	372:414	The purified EPS had an average molecular weight of 1.1 × 106 Da.					
29337101	1	45	from	production	111:120	arg1	confusa					200:206	confusa	200:206	confusa	200:206	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	1	46	theme	in-vitro	222:229	arg1	potentials					241:250	the in-vitro probiotic potentials	218:250	the in-vitro probiotic potentials of this strain	218:265	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	11	47	theme	experimental	1176:1187	arg1	3.00 g/L					1199:1206	3.00 g/L	1199:1206	3.00 g/L	1199:1206	The EPS produced was predicted to be 3.10 g/L, while the experimental yield was 3.00 g/L.					
29337101	11	47	theme	experimental	1176:1187	arg1	yield					1189:1193	the experimental yield	1172:1193	the experimental yield	1172:1193	The EPS produced was predicted to be 3.10 g/L, while the experimental yield was 3.00 g/L.					
29337101	12	48	theme	desirable	1242:1250	arg1	attributes					1262:1271	desirable probiotic attributes	1242:1271	desirable probiotic attributes	1242:1271	This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h.					
29337101	6	49	theme	N-acetyl	670:677	arg1	presence					638:645	the presence	634:645	the presence of hydroxyl, carboxyl, N-acetyl and amine groups	634:694	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
29337101	1	50	theme	probiotic	231:239	arg1	potentials					241:250	the in-vitro probiotic potentials	218:250	the in-vitro probiotic potentials of this strain	218:265	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	0	51	dep	polysaccharide	14:27	arg1	optimization					71:82	optimization	71:82	optimization	71:82	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.					
29337101	0	51	dep	polysaccharide	14:27	arg1	characterization					89:104	characterization	89:104	characterization	89:104	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.					
29337101	0	51	dep	polysaccharide	14:27	arg1	Production					59:68	Production	59:68	Production	59:68	Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.					
29337101	5	52	theme	EPS	554:556	arg1	characteristics					531:545	The structural characteristics	516:545	The structural characteristics of the EPS	516:556	The structural characteristics of the EPS were investigated by FTIR, and NMR spectroscopy.					
29337101	8	53	theme	in-vitro	815:822	arg1	activity					836:843	strong in-vitro antioxidant activity	808:843	strong in-vitro antioxidant activity	808:843	The EPS showed strong in-vitro antioxidant activity.					
29337101	13	54	from	applications	1494:1505	arg1	industries					1510:1519	industries	1510:1519	industries	1510:1519	The results obtained from this study demonstrate W. confusa OF126 as a promising probiotic and the EPS produced can find useful applications in industries.					
29337101	10	55	theme	optimum	977:983	arg1	conditions					985:994	The predicted optimum conditions	963:994	The predicted optimum conditions for EPS production	963:1013	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	10	55	theme	optimum	977:983	arg1	time					1032:1035	cultivation time	1020:1035	cultivation time (48.50 h)	1020:1045	The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).					
29337101	1	56	dep	production	111:120	arg1	The					107:109	The	107:109	The	107:109	The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated.					
29337101	6	57	theme	carboxyl	660:667	arg1	presence					638:645	the presence	634:645	the presence of hydroxyl, carboxyl, N-acetyl and amine groups	634:694	FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.					
30316197	0	0	theme	Eucalyptus	90:99	arg1	hydrolysis					76:85	enzymatic hydrolysis	66:85	enzymatic hydrolysis of Eucalyptus and its mechanism	66:117	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	4	1	theme	region	829:834	arg1	destruction					802:812	destruction	802:812	destruction of crystalline region	802:834	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	1	theme	region	829:834	arg1	micro-morphology					871:886	an eroded, pored and irregular micro-morphology	840:886	an eroded, pored and irregular micro-morphology	840:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	1	theme	region	829:834	arg1	removal					775:781	removal	775:781	removal of hemicelluloses	775:799	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	1	theme	region	829:834	arg1	lignin					767:772	lignin	767:772	lignin	767:772	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	5	2	theme	great	1003:1007	arg1	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	2	theme	great	1003:1007	arg1	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	1	3	theme	enzymatic	239:247	arg1	hydrolysis					249:258	enzymatic hydrolysis	239:258	enzymatic hydrolysis of Eucalyptus sawdust	239:280	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	4	4	theme	crystalline	817:827	arg1	region					829:834	crystalline region	817:834	crystalline region	817:834	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	5	5	theme	efficient	933:941	arg1	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	5	theme	efficient	933:941	arg1	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	2	6	theme	sugar	287:291	arg1	yield					293:297	The sugar yield	283:297	The sugar yield of pretreated sample	283:318	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	4	7	theme	[TBA	718:721	arg1	[OH					723:725	microwave and [TBA][OH]	704:726	[OH	723:725	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	5	8	theme	inexpensive	912:922	arg1	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	8	theme	inexpensive	912:922	arg1	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	3	9	theme	state	581:585	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	9	theme	state	581:585	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	4	10	theme	[OH	723:725	arg1	effect					694:699	The combined effect	681:699	The combined effect of microwave and [TBA][OH]	681:726	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	11	theme	polarization/magic-angle	541:564	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	11	theme	polarization/magic-angle	541:564	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	1	12	theme	Eucalyptus	263:272	arg1	sawdust					274:280	Eucalyptus sawdust	263:280	Eucalyptus sawdust	263:280	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	5	13	theme	green	894:898	arg1	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	13	theme	green	894:898	arg1	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	14	theme	high	928:931	arg1	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	14	theme	high	928:931	arg1	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	2	15	theme	microwave-assisted	380:397	arg1	pretreatment					409:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	1	16	theme	microwave	174:182	arg1	[OH					206:208	microwave and ionic liquid [TBA][OH]	174:209	[OH	206:208	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	0	17	theme	mechanism	109:117	arg1	hydrolysis					76:85	enzymatic hydrolysis	66:85	enzymatic hydrolysis of Eucalyptus and its mechanism	66:117	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	3	18	theme	work	427:430	arg1	mechanism					432:440	The work mechanism	423:440	The work mechanism	423:440	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	19	theme	13C	531:533	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	19	theme	13C	531:533	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	2	20	theme	[TBA	399:402	arg1	pretreatment					409:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	0	21	theme	microwave-assisted	2:19	arg1	pretreatment					42:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	2	22	theme	sample	313:318	arg1	yield					293:297	The sugar yield	283:297	The sugar yield of pretreated sample	283:318	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	1	23	theme	ionic	188:192	arg1	[OH					206:208	microwave and ionic liquid [TBA][OH]	174:209	[OH	206:208	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	1	24	theme	sawdust	274:280	arg1	hydrolysis					249:258	enzymatic hydrolysis	239:258	enzymatic hydrolysis of Eucalyptus sawdust	239:280	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	4	25	theme	destruction	802:812	arg1	deconstruction					749:762	the violent deconstruction	737:762	the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology	737:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	2	26	theme	pretreated	302:311	arg1	sample					313:318	pretreated sample	302:318	pretreated sample	302:318	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	1	27	theme	liquid	194:199	arg1	[OH					206:208	microwave and ionic liquid [TBA][OH]	174:209	[OH	206:208	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	0	28	theme	ionic	29:33	arg1	pretreatment					42:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	4	29	theme	microwave	704:712	arg1	[OH					723:725	microwave and [TBA][OH]	704:726	[OH	723:725	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	30	theme	micro-morphology	871:886	arg1	deconstruction					749:762	the violent deconstruction	737:762	the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology	737:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	31	theme	removal	775:781	arg1	deconstruction					749:762	the violent deconstruction	737:762	the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology	737:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	32	theme	spinning	566:573	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	32	theme	spinning	566:573	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	1	33	theme	[TBA	201:204	arg1	[OH					206:208	microwave and ionic liquid [TBA][OH]	174:209	[OH	206:208	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	0	34	theme	aqueous	21:27	arg1	pretreatment					42:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	4	35	theme	irregular	861:869	arg1	micro-morphology					871:886	an eroded, pored and irregular micro-morphology	840:886	an eroded, pored and irregular micro-morphology	840:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	36	theme	solid	575:579	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	36	theme	solid	575:579	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	37	theme	infrared	501:508	arg1	FTIR					524:527	FTIR	524:527	FTIR	524:527	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	37	theme	infrared	501:508	arg1	spectroscopy					510:521	infrared spectroscopy	501:521	infrared spectroscopy (FTIR)	501:528	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	38	theme	solid	596:600	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	38	theme	solid	596:600	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	2	39	theme	optimized	370:378	arg1	pretreatment					409:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	1	40	theme	[OH	206:208	arg1	combination					159:169	combination	159:169	combination of microwave and ionic liquid [TBA][OH]	159:209	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	0	41	theme	liquid	35:40	arg1	pretreatment					42:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment	0:53	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	3	42	dep	spectroscopy	510:521	arg1	analyses					671:678	analyses	671:678	analyses	671:678	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	4	43	theme	lignin	767:772	arg1	deconstruction					749:762	the violent deconstruction	737:762	the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology	737:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	2	44	theme	[OH	404:406	arg1	pretreatment					409:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	optimized microwave-assisted [TBA][OH] pretreatment	370:420	The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment.					
30316197	5	45	theme	bio-refinery	1035:1046	arg1	field					1026:1030	the field	1022:1030	the field of bio-refinery	1022:1046	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	4	46	theme	combined	685:692	arg1	effect					694:699	The combined effect	681:699	The combined effect of microwave and [TBA][OH]	681:726	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	5	47	contain	has	999:1001	arg2	pretreatment					943:954	a green, relatively inexpensive and high efficient pretreatment	892:954	a green, relatively inexpensive and high efficient pretreatment	892:954	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	47	contain	has	999:1001	arg2	potential					1009:1017	great potential	1003:1017	great potential	1003:1017	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	5	47	contain	has	999:1001	arg1	pretreatment					986:997	microwave-assisted [TBA][OH] pretreatment	957:997	microwave-assisted [TBA][OH] pretreatment	957:997	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	4	48	theme	hemicelluloses	786:799	arg1	destruction					802:812	destruction	802:812	destruction of crystalline region	802:834	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	48	theme	hemicelluloses	786:799	arg1	micro-morphology					871:886	an eroded, pored and irregular micro-morphology	840:886	an eroded, pored and irregular micro-morphology	840:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	48	theme	hemicelluloses	786:799	arg1	removal					775:781	removal	775:781	removal of hemicelluloses	775:799	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	48	theme	hemicelluloses	786:799	arg1	lignin					767:772	lignin	767:772	lignin	767:772	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	4	49	theme	eroded	843:848	arg1	micro-morphology					871:886	an eroded, pored and irregular micro-morphology	840:886	an eroded, pored and irregular micro-morphology	840:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	50	theme	13C	592:594	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	50	theme	13C	592:594	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	1	51	theme	novel	122:126	arg1	strategy					141:148	A novel pretreatment strategy	120:148	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH]	120:209	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	4	52	theme	violent	741:747	arg1	deconstruction					749:762	the violent deconstruction	737:762	the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology	737:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	53	theme	X-ray	608:612	arg1	XRD					627:629	XRD	627:629	XRD	627:629	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	53	theme	X-ray	608:612	arg1	diffraction					614:624	X-ray diffraction	608:624	X-ray diffraction (XRD)	608:630	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	5	54	theme	[OH	981:983	arg1	pretreatment					986:997	microwave-assisted [TBA][OH] pretreatment	957:997	microwave-assisted [TBA][OH] pretreatment	957:997	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	1	55	theme	pretreatment	128:139	arg1	strategy					141:148	A novel pretreatment strategy	120:148	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH]	120:209	A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust.					
30316197	4	56	theme	pored	851:855	arg1	micro-morphology					871:886	an eroded, pored and irregular micro-morphology	840:886	an eroded, pored and irregular micro-morphology	840:886	The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology.					
30316197	3	57	theme	scanning	636:643	arg1	SEM					666:668	SEM	666:668	SEM	666:668	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	57	theme	scanning	636:643	arg1	microscope					654:663	scanning electron microscope	636:663	scanning electron microscope (SEM)	636:669	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	58	theme	cross	535:539	arg1	NMR					602:604	13C solid NMR	592:604	13C solid NMR	592:604	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	58	theme	cross	535:539	arg1	NMR					587:589	13C cross polarization/magic-angle spinning solid state NMR	531:589	13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR)	531:605	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	59	theme	electron	645:652	arg1	SEM					666:668	SEM	666:668	SEM	666:668	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	3	59	theme	electron	645:652	arg1	microscope					654:663	scanning electron microscope	636:663	scanning electron microscope (SEM)	636:669	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	5	60	theme	microwave-assisted	957:974	arg1	pretreatment					986:997	microwave-assisted [TBA][OH] pretreatment	957:997	microwave-assisted [TBA][OH] pretreatment	957:997	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
30316197	3	61	theme	chemical	461:468	arg1	composition					470:480	chemical composition	461:480	chemical composition	461:480	The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), 13C cross polarization/magic-angle spinning solid state NMR (13C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses.					
30316197	0	62	theme	enzymatic	66:74	arg1	hydrolysis					76:85	enzymatic hydrolysis	66:85	enzymatic hydrolysis of Eucalyptus and its mechanism	66:117	A microwave-assisted aqueous ionic liquid pretreatment to enhance enzymatic hydrolysis of Eucalyptus and its mechanism.					
30316197	5	63	theme	[TBA	976:979	arg1	pretreatment					986:997	microwave-assisted [TBA][OH] pretreatment	957:997	microwave-assisted [TBA][OH] pretreatment	957:997	As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.					
29961784	8	0	dep	prevention	1601:1610	arg1	the					1597:1599	the	1597:1599	the	1597:1599	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	1	1	theme	biological	138:147	arg1	functions					149:157	the biological functions	134:157	the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88	134:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	0	2	theme	K88	71:73	arg1	adhesion					54:61	the adhesion	50:61	the adhesion of ETEC K88 to intestinal epithelial cells	50:104	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	7	3	theme	green	1270:1274	arg1	olives					1276:1281	table green olives	1264:1281	table green olives	1264:1281	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	6	4	theme	displacement	1076:1087	arg1	test					1089:1092	the displacement test	1072:1092	the displacement test	1072:1092	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	2	5	theme	industrial	342:351	arg1	fermenters					353:362	five industrial fermenters	337:362	five industrial fermenters	337:362	Exopolysaccharides were isolated from five industrial fermenters.					
29961784	1	6	theme	olives	248:253	arg1	fermentation					232:243	the industrial fermentation	217:243	the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88	217:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	0	7	theme	epithelial	89:98	arg1	cells					100:104	intestinal epithelial cells	78:104	intestinal epithelial cells	78:104	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	7	8	theme	olives	1276:1281	arg1	fermentation					1248:1259	the fermentation	1244:1259	the fermentation of table green olives	1244:1281	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	4	9	theme	shift	760:764	arg1	values					766:771	chemicals shift values	750:771	chemicals shift values	750:771	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	8	10	theme	functional	1425:1434	arg1	properties					1436:1445	novel functional properties	1419:1445	novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics	1419:1552	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	6	11	theme	K88	1029:1031	arg1	adhesion					1033:1040	the ETEC K88 adhesion	1020:1040	the ETEC K88 adhesion to IPEC-J2 cells	1020:1057	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	8	12	theme	isolated	1509:1516	arg1	EPSs					1518:1521	the isolated EPSs	1505:1521	the isolated EPSs as candidates for nutraceutics	1505:1552	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	7	13	theme	intestinal	1355:1364	arg1	cells					1377:1381	the intestinal epithelial cells	1351:1381	the intestinal epithelial cells	1351:1381	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	0	14	theme	intestinal	78:87	arg1	cells					100:104	intestinal epithelial cells	78:104	intestinal epithelial cells	78:104	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	5	15	theme	variable	943:950	arg1	intensities					952:962	variable intensities	943:962	variable intensities	943:962	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	4	16	theme	chemicals	750:758	arg1	values					766:771	chemicals shift values	750:771	chemicals shift values	750:771	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	8	17	theme	diarrhoea	1634:1642	arg1	treatment					1616:1624	treatment	1616:1624	treatment	1616:1624	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	8	17	theme	diarrhoea	1634:1642	arg1	prevention					1601:1610	prevention	1601:1610	prevention	1601:1610	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	6	18	theme	ETEC	1024:1027	arg1	adhesion					1033:1040	the ETEC K88 adhesion	1020:1040	the ETEC K88 adhesion to IPEC-J2 cells	1020:1057	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	1	19	theme	enterotoxigenic	263:277	arg1	K88					294:296	enterotoxigenic E. coli (ETEC) K88	263:296	enterotoxigenic E. coli (ETEC) K88	263:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	7	20	theme	table	1264:1268	arg1	olives					1276:1281	table green olives	1264:1281	table green olives	1264:1281	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	4	21	from	presence	659:666	arg1	form					692:695	the form	688:695	the form of α-d-Glcp-(1→) and two different α-furanoses	688:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	22	theme	α-pyranose	674:683	arg1	presence					659:666	the presence	655:666	the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses	655:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	8	23	theme	novel	1419:1423	arg1	properties					1436:1445	novel functional properties	1419:1445	novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics	1419:1552	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	7	24	theme	epithelial	1366:1375	arg1	cells					1377:1381	the intestinal epithelial cells	1351:1381	the intestinal epithelial cells	1351:1381	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	3	25	theme	monosaccharide	383:396	arg1	composition					398:408	their monosaccharide composition	377:408	their monosaccharide composition	377:408	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	2	26	attach	isolated	323:330	arg2	Exopolysaccharides					299:316	Exopolysaccharides	299:316	Exopolysaccharides	299:316	Exopolysaccharides were isolated from five industrial fermenters.					
29961784	2	26	attach	isolated	323:330	arg1	fermenters					353:362	five industrial fermenters	337:362	five industrial fermenters	337:362	Exopolysaccharides were isolated from five industrial fermenters.					
29961784	4	27	theme	α-furanoses	732:742	arg1	form					692:695	the form	688:695	the form of α-d-Glcp-(1→) and two different α-furanoses	688:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	5	28	with	K88	923:925	arg1	intensities					952:962	variable intensities	943:962	variable intensities	943:962	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	1	29	theme	isolated	166:173	arg1	EPSs					195:198	EPSs	195:198	EPSs	195:198	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	1	29	theme	isolated	166:173	arg1	exopolysaccharides					175:192	the isolated exopolysaccharides	162:192	the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88	162:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	3	30	theme	composition	398:408	arg1	Analysis					365:372	Analysis	365:372	Analysis of their monosaccharide composition by GLC	365:415	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	4	31	attach	presence	659:666	arg2	α-pyranose					674:683	an α-pyranose	671:683	an α-pyranose	671:683	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	31	attach	presence	659:666	arg1	form					692:695	the form	688:695	the form of α-d-Glcp-(1→) and two different α-furanoses	688:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	32	theme	different	722:730	arg1	α-furanoses					732:742	two different α-furanoses	718:742	two different α-furanoses	718:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	33	theme	in-depth	628:635	arg1	analysis					637:644	a more in-depth analysis	621:644	a more in-depth analysis	621:644	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	5	34	dep	in	837:838	arg1	vitro					840:844	vitro	840:844	vitro	840:844	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	3	35	theme	main	435:438	arg1	components					440:449	the main components	431:449	the main components	431:449	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	3	35	theme	main	435:438	arg1	glucose					456:462	glucose	456:462	glucose (27%-50%)	456:472	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	1	36	theme	coli	282:285	arg1	K88					294:296	enterotoxigenic E. coli (ETEC) K88	263:296	enterotoxigenic E. coli (ETEC) K88	263:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	8	37	theme	ETEC	1629:1632	arg1	diarrhoea					1634:1642	ETEC diarrhoea	1629:1642	ETEC diarrhoea	1629:1642	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	5	38	theme	Miniaturized	824:835	arg1	tests					846:850	Miniaturized in vitro tests	824:850	Miniaturized in vitro tests	824:850	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	0	39	theme	olive	24:28	arg1	brines					30:35	olive brines	24:35	olive brines	24:35	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	5	40	theme	in	837:838	arg1	tests					846:850	Miniaturized in vitro tests	824:850	Miniaturized in vitro tests	824:850	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	3	41	theme	%	467:467	arg1	%					471:471	27%-50%	465:471	27%-50%	465:471	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	3	41	theme	%	467:467	arg1	glucose					456:462	glucose	456:462	glucose (27%-50%)	456:472	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	1	42	theme	exopolysaccharides	175:192	arg1	functions					149:157	the biological functions	134:157	the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88	134:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	4	43	theme	NMR	557:559	arg1	spectrum					561:568	The 1H NMR spectrum	550:568	The 1H NMR spectrum	550:568	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	44	theme	α-d-Glcf	801:808	arg1	presence					789:796	the presence	785:796	the presence of α-d-Glcf and α-d-Galf	785:821	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	7	45	theme	opportunistic	1322:1334	arg1	pathogens					1336:1344	opportunistic pathogens	1322:1344	opportunistic pathogens	1322:1344	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	4	46	theme	α-d-Glcp-	700:708	arg1	form					692:695	the form	688:695	the form of α-d-Glcp-(1→) and two different α-furanoses	688:742	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	4	47	theme	1H	554:555	arg1	spectrum					561:568	The 1H NMR spectrum	550:568	The 1H NMR spectrum	550:568	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	6	48	theme	P	1181:1181	arg1	cells					1174:1178	the cells	1170:1178	the cells (P < 0.01)	1170:1189	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	6	48	theme	P	1181:1181	arg1	<					1183:1183	P < 0.01	1181:1188	P < 0.01	1181:1188	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	7	49	theme	pathogens	1336:1344	arg1	attachment					1308:1317	the attachment	1304:1317	the attachment of opportunistic pathogens onto the intestinal epithelial cells	1304:1381	These results suggest that the EPSs produced during the fermentation of table green olives could interfere with the attachment of opportunistic pathogens onto the intestinal epithelial cells.					
29961784	8	50	theme	human	1568:1572	arg1	diets					1588:1592	human and/or animal diets	1568:1592	human and/or animal diets	1568:1592	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	5	51	theme	ETEC	918:921	arg1	K88					923:925	ETEC K88	918:925	ETEC K88 (P < 0.05) with variable intensities	918:962	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	5	51	theme	ETEC	918:921	arg1	<					930:930	P < 0.05	928:935	P < 0.05	928:935	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	6	52	theme	EPS	1099:1101	arg1	samples					1103:1109	all EPS samples	1095:1109	all EPS samples	1095:1109	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	8	53	theme	fermented	1482:1490	arg1	food					1492:1495	this traditional Mediterranean fermented food	1451:1495	this traditional Mediterranean fermented food	1451:1495	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	8	54	theme	properties	1436:1445	arg1	possibility					1404:1414	the possibility	1400:1414	the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea	1400:1642	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	3	55	dep	glucose	456:462	arg1	followed					498:505	followed	498:505	followed by rhamnose (4-23%) and arabinose (6-17%)	498:547	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	8	56	theme	animal	1581:1586	arg1	diets					1588:1592	human and/or animal diets	1568:1592	human and/or animal diets	1568:1592	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	4	57	theme	more	623:626	arg1	analysis					637:644	a more in-depth analysis	621:644	a more in-depth analysis	621:644	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	6	58	theme	competition	969:979	arg1	test					981:984	The competition test	965:984	The competition test	965:984	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	6	59	theme	IPEC-J2	1045:1051	arg1	cells					1053:1057	IPEC-J2 cells	1045:1057	IPEC-J2 cells	1045:1057	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	4	60	theme	similar	584:590	arg1	profile					592:598	a very similar profile	577:598	a very similar profile between samples	577:614	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
29961784	0	61	theme	ETEC	66:69	arg1	K88					71:73	ETEC K88	66:73	ETEC K88	66:73	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	5	62	theme	P	928:928	arg1	K88					923:925	ETEC K88	918:925	ETEC K88 (P < 0.05) with variable intensities	918:962	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	5	62	theme	P	928:928	arg1	<					930:930	P < 0.05	928:935	P < 0.05	928:935	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	5	63	theme	EPS	880:882	arg1	samples					884:890	EPS samples	880:890	EPS samples	880:890	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	8	64	theme	Mediterranean	1468:1480	arg1	food					1492:1495	this traditional Mediterranean fermented food	1451:1495	this traditional Mediterranean fermented food	1451:1495	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	1	65	theme	industrial	221:230	arg1	fermentation					232:243	the industrial fermentation	217:243	the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88	217:296	This study aims to explore the biological functions of the isolated exopolysaccharides (EPSs) produced during the industrial fermentation of olives against enterotoxigenic E. coli (ETEC) K88.					
29961784	8	66	theme	traditional	1456:1466	arg1	food					1492:1495	this traditional Mediterranean fermented food	1451:1495	this traditional Mediterranean fermented food	1451:1495	This would open the possibility of novel functional properties for this traditional Mediterranean fermented food and for the isolated EPSs as candidates for nutraceutics to be used in human and/or animal diets in the prevention and treatment of ETEC diarrhoea.					
29961784	0	67	from	brines	30:35	arg1	Exopolysaccharides					0:17	Exopolysaccharides	0:17	Exopolysaccharides from olive brines	0:35	Exopolysaccharides from olive brines could reduce the adhesion of ETEC K88 to intestinal epithelial cells.					
29961784	5	68	theme	samples	884:890	arg1	ability					869:875	the ability	865:875	the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities	865:962	Miniaturized in vitro tests demonstrated the ability of EPS samples to attach specifically to ETEC K88 (P < 0.05) with variable intensities.					
29961784	3	69	theme	%	491:491	arg1	%					495:495	23%-33%	489:495	23%-33%	489:495	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	3	69	theme	%	491:491	arg1	galactose					478:486	galactose	478:486	galactose (23%-33%)	478:496	Analysis of their monosaccharide composition by GLC revealed that the main components were glucose (27%-50%) and galactose (23%-33%) followed by rhamnose (4-23%) and arabinose (6-17%).					
29961784	6	70	attach	attached	1158:1165	arg2	pathogens					1148:1156	the pathogens	1144:1156	the pathogens attached to the cells (P < 0.01)	1144:1189	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	6	70	attach	attached	1158:1165	arg1	cells					1174:1178	the cells	1170:1178	the cells (P < 0.01)	1170:1189	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	6	70	attach	attached	1158:1165	arg1	<					1183:1183	P < 0.01	1181:1188	P < 0.01	1181:1188	The competition test did not show the ability to block the ETEC K88 adhesion to IPEC-J2 cells; however, in the displacement test, all EPS samples were shown to effectively remove the pathogens attached to the cells (P < 0.01).					
29961784	4	71	theme	α-d-Galf	814:821	arg1	presence					789:796	the presence	785:796	the presence of α-d-Glcf and α-d-Galf	785:821	The 1H NMR spectrum showed a very similar profile between samples, and a more in-depth analysis revealed the presence of an α-pyranose in the form of α-d-Glcp-(1→) and two different α-furanoses, with chemicals shift values, suggesting the presence of α-d-Glcf and α-d-Galf.					
30036629	9	0	theme	amygdalus	1266:1274	arg1	peels					1276:1280	the Prunus amygdalus peels	1255:1280	the Prunus amygdalus peels	1255:1280	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	5	1	theme	extracted	671:679	arg1	yield					681:685	an extracted yield	668:685	an extracted yield of 9%	668:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	9	2	theme	activity	1141:1148	arg1	result					1109:1114	the result	1105:1114	the result of the antiproliferative activity against Caco-2 and B-16 cells	1105:1178	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	3	3	from	shell	414:418	arg1	polysaccharides					376:390	polysaccharides	376:390	polysaccharides from Prunus amygdalus shell	376:418	In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid.					
30036629	8	4	theme	antioxidant	1071:1081	arg1	activities					1083:1092	even better antioxidant activities	1059:1092	even better antioxidant activities	1059:1092	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	0	5	theme	antiproliferative	81:97	arg1	activities					99:108	Antioxidant and antiproliferative activities	65:108	Antioxidant and antiproliferative activities	65:108	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	2	6	theme	pharmacological	334:348	arg1	activities					350:359	their biological and pharmacological activities	313:359	their biological and pharmacological activities	313:359	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	9	7	theme	strong	1206:1211	arg1	ability					1226:1232	strong cytotoxicity ability	1206:1232	strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food	1206:1352	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	0	8	from	Characterization	0:15	arg1	peels					58:62	Prunus amygdalus peels	41:62	Prunus amygdalus peels	41:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	6	9	theme	polysaccharides	803:817	arg1	%					840:840	88.57%	835:840	88.57%	835:840	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	9	theme	polysaccharides	803:817	arg1	extractability					819:832	the highest polysaccharides extractability	791:832	the highest polysaccharides extractability (88.57%)	791:841	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	9	10	theme	functional	1338:1347	arg1	food					1349:1352	functional food	1338:1352	functional food	1338:1352	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	6	11	theme	highest	795:801	arg1	%					840:840	88.57%	835:840	88.57%	835:840	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	11	theme	highest	795:801	arg1	extractability					819:832	the highest polysaccharides extractability	791:832	the highest polysaccharides extractability (88.57%)	791:841	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	8	12	dep	tests	1005:1009	arg1	FRAP					1027:1030	FRAP	1027:1030	FRAP	1027:1030	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	12	dep	tests	1005:1009	arg1	ABTS					1018:1021	ABTS	1018:1021	ABTS	1018:1021	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	12	dep	tests	1005:1009	arg1	DPPH					1012:1015	DPPH	1012:1015	DPPH	1012:1015	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	12	dep	tests	1005:1009	arg1	tests					1005:1009	The antioxidant tests	989:1009	The antioxidant tests (DPPH, ABTS and FRAP)	989:1031	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	3	13	theme	hydrochloric	479:490	arg1	acid					492:495	hydrochloric acid	479:495	hydrochloric acid	479:495	In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid.					
30036629	0	14	from	peels	58:62	arg1	polysaccharides					20:34	polysaccharides	20:34	polysaccharides from Prunus amygdalus peels	20:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	0	14	from	peels	58:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharides from Prunus amygdalus peels	0:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	5	15	theme	extracting	646:655	arg1	polysaccharide					596:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	5	15	theme	extracting	646:655	arg1	agent					657:661	the most effective extracting agent	627:661	the most effective extracting agent with an extracted yield of 9%	627:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	5	16	theme	effective	636:644	arg1	polysaccharide					596:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	5	16	theme	effective	636:644	arg1	agent					657:661	the most effective extracting agent	627:661	the most effective extracting agent with an extracted yield of 9%	627:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	6	17	theme	esterification	868:881	arg1	%					840:840	88.57%	835:840	88.57%	835:840	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	17	theme	esterification	868:881	arg1	content					773:779	the highest galacturonic acid content	743:779	the highest galacturonic acid content (31.95%)	743:788	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	17	theme	esterification	868:881	arg1	extractability					819:832	the highest polysaccharides extractability	791:832	the highest polysaccharides extractability (88.57%)	791:841	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	17	theme	esterification	868:881	arg1	%					787:787	31.95%	782:787	31.95%	782:787	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	17	theme	esterification	868:881	arg1	%					889:889	31.76%	884:889	31.76%	884:889	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	17	theme	esterification	868:881	arg1	degree					858:863	the lowest degree	847:863	the lowest degree of esterification (31.76%)	847:890	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	0	18	theme	amygdalus	48:56	arg1	peels					58:62	Prunus amygdalus peels	41:62	Prunus amygdalus peels	41:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	2	19	dep	interested	244:253	arg1	evaluate					304:311	evaluate	304:311	to evaluate their biological and pharmacological activities	301:359	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	2	19	dep	interested	244:253	arg1	valorize					258:265	valorize	258:265	to valorize almond waste (hull and shell)	255:295	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	9	20	theme	raw	1307:1309	arg1	materials					1311:1319	the natural raw materials	1295:1319	the natural raw materials for new drug and functional food	1295:1352	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	0	21	dep	activities	99:108	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharides from Prunus amygdalus peels	0:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	6	22	theme	Soluble	703:709	arg1	Polysaccharides					711:725	The acid Soluble Polysaccharides	694:725	The acid Soluble Polysaccharides (ASP)	694:731	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	22	theme	Soluble	703:709	arg1	ASP					728:730	ASP	728:730	ASP	728:730	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	7	23	theme	different	897:905	arg1	fractions					923:931	The different polysaccharides fractions	893:931	The different polysaccharides fractions	893:931	The different polysaccharides fractions were characterized by FTIR, 1H NMR and SEC/MALS/VD/DRI.					
30036629	1	24	theme	Prunus	111:116	arg1	amygdalus					118:126	Prunus amygdalus	111:126	Prunus amygdalus	111:126	Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases.					
30036629	6	25	theme	acid	698:701	arg1	Polysaccharides					711:725	The acid Soluble Polysaccharides	694:725	The acid Soluble Polysaccharides (ASP)	694:731	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	25	theme	acid	698:701	arg1	ASP					728:730	ASP	728:730	ASP	728:730	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	1	26	theme	plant	190:194	arg1	interest					173:180	the interest	169:180	the interest of this plant which cures many diseases	169:220	Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases.					
30036629	7	27	theme	polysaccharides	907:921	arg1	fractions					923:931	The different polysaccharides fractions	893:931	The different polysaccharides fractions	893:931	The different polysaccharides fractions were characterized by FTIR, 1H NMR and SEC/MALS/VD/DRI.					
30036629	3	28	theme	ammonium	458:465	arg1	oxalate					467:473	ammonium oxalate	458:473	ammonium oxalate	458:473	In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid.					
30036629	0	29	theme	polysaccharides	20:34	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharides from Prunus amygdalus peels	0:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	9	30	theme	new	1325:1327	arg1	drug					1329:1332	new drug	1325:1332	new drug	1325:1332	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	1	31	used	used	131:134	arg2	amygdalus					118:126	Prunus amygdalus	111:126	Prunus amygdalus	111:126	Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases.					
30036629	9	32	theme	Prunus	1259:1264	arg1	peels					1276:1280	the Prunus amygdalus peels	1255:1280	the Prunus amygdalus peels	1255:1280	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	3	33	theme	amygdalus	404:412	arg1	shell					414:418	Prunus amygdalus shell	397:418	Prunus amygdalus shell	397:418	In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid.					
30036629	6	34	theme	lowest	851:856	arg1	%					889:889	31.76%	884:889	31.76%	884:889	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	34	theme	lowest	851:856	arg1	degree					858:863	the lowest degree	847:863	the lowest degree of esterification (31.76%)	847:890	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	1	35	theme	many	208:211	arg1	diseases					213:220	many diseases	208:220	many diseases	208:220	Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases.					
30036629	0	36	theme	Prunus	41:46	arg1	peels					58:62	Prunus amygdalus peels	41:62	Prunus amygdalus peels	41:62	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	4	37	theme	fractions	548:556	arg1	composition					517:527	The monosaccharide composition	498:527	The monosaccharide composition of polysaccharides fractions	498:556	The monosaccharide composition of polysaccharides fractions was performed by GC-MS.					
30036629	9	38	theme	cytotoxicity	1213:1224	arg1	ability					1226:1232	strong cytotoxicity ability	1206:1232	strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food	1206:1352	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	4	39	theme	polysaccharides	532:546	arg1	fractions					548:556	polysaccharides fractions	532:556	polysaccharides fractions	532:556	The monosaccharide composition of polysaccharides fractions was performed by GC-MS.					
30036629	5	40	theme	%	691:691	arg1	yield					681:685	an extracted yield	668:685	an extracted yield of 9%	668:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	2	41	theme	Many	223:226	arg1	researchers					228:238	Many researchers	223:238	Many researchers	223:238	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	9	42	theme	natural	1299:1305	arg1	materials					1311:1319	the natural raw materials	1295:1319	the natural raw materials for new drug and functional food	1295:1352	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	3	43	theme	Prunus	397:402	arg1	shell					414:418	Prunus amygdalus shell	397:418	Prunus amygdalus shell	397:418	In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid.					
30036629	8	44	theme	better	1064:1069	arg1	activities					1083:1092	even better antioxidant activities	1059:1092	even better antioxidant activities	1059:1092	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	6	45	theme	acid	768:771	arg1	content					773:779	the highest galacturonic acid content	743:779	the highest galacturonic acid content (31.95%)	743:788	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	45	theme	acid	768:771	arg1	%					787:787	31.95%	782:787	31.95%	782:787	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	2	46	theme	biological	319:328	arg1	activities					350:359	their biological and pharmacological activities	313:359	their biological and pharmacological activities	313:359	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	8	47	theme	antioxidant	993:1003	arg1	FRAP					1027:1030	FRAP	1027:1030	FRAP	1027:1030	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	47	theme	antioxidant	993:1003	arg1	ABTS					1018:1021	ABTS	1018:1021	ABTS	1018:1021	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	47	theme	antioxidant	993:1003	arg1	DPPH					1012:1015	DPPH	1012:1015	DPPH	1012:1015	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	8	47	theme	antioxidant	993:1003	arg1	tests					1005:1009	The antioxidant tests	989:1009	The antioxidant tests (DPPH, ABTS and FRAP)	989:1031	The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities.					
30036629	2	48	theme	almond	267:272	arg1	waste					274:278	almond waste	267:278	almond waste (hull and shell)	267:295	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	5	49	theme	Water-soluble	582:594	arg1	polysaccharide					596:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide	582:609	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	5	49	theme	Water-soluble	582:594	arg1	agent					657:661	the most effective extracting agent	627:661	the most effective extracting agent with an extracted yield of 9%	627:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
30036629	9	50	theme	antiproliferative	1123:1139	arg1	activity					1141:1148	the antiproliferative activity	1119:1148	the antiproliferative activity against Caco-2 and B-16 cells	1119:1178	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	6	51	theme	galacturonic	755:766	arg1	content					773:779	the highest galacturonic acid content	743:779	the highest galacturonic acid content (31.95%)	743:788	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	51	theme	galacturonic	755:766	arg1	%					787:787	31.95%	782:787	31.95%	782:787	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	52	theme	highest	747:753	arg1	content					773:779	the highest galacturonic acid content	743:779	the highest galacturonic acid content (31.95%)	743:788	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	6	52	theme	highest	747:753	arg1	%					787:787	31.95%	782:787	31.95%	782:787	The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%).					
30036629	1	53	theme	folk	143:146	arg1	medicine					148:155	the folk medicine	139:155	the folk medicine that proved the interest of this plant which cures many diseases	139:220	Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases.					
30036629	9	54	theme	B-16	1169:1172	arg1	cells					1174:1178	B-16 cells	1169:1178	B-16 cells	1169:1178	Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.					
30036629	0	55	theme	Antioxidant	65:75	arg1	activities					99:108	Antioxidant and antiproliferative activities	65:108	Antioxidant and antiproliferative activities	65:108	Characterization of polysaccharides from Prunus amygdalus peels: Antioxidant and antiproliferative activities.					
30036629	4	56	theme	monosaccharide	502:515	arg1	composition					517:527	The monosaccharide composition	498:527	The monosaccharide composition of polysaccharides fractions	498:556	The monosaccharide composition of polysaccharides fractions was performed by GC-MS.					
30036629	7	57	theme	1H	961:962	arg1	NMR					964:966	1H NMR	961:966	1H NMR	961:966	The different polysaccharides fractions were characterized by FTIR, 1H NMR and SEC/MALS/VD/DRI.					
30036629	2	58	dep	waste	274:278	arg1	hull					281:284	hull	281:284	hull	281:284	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	2	58	dep	waste	274:278	arg1	shell					290:294	shell	290:294	shell	290:294	Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities.					
30036629	5	59	with	agent	657:661	arg1	yield					681:685	an extracted yield	668:685	an extracted yield of 9%	668:691	Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%.					
31159355	6	0	theme	rounded	857:863	arg1	morphology					877:886	rounded or dumbbell morphology	857:886	rounded or dumbbell morphology	857:886	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	7	1	theme	flow	933:936	arg1	cytometry					938:946	flow cytometry	933:946	flow cytometry (FITC-conjugated SPs)	933:968	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	7	1	theme	flow	933:936	arg1	SPs					965:967	FITC-conjugated SPs	949:967	FITC-conjugated SPs	949:967	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	7	2	theme	fluorescence	908:919	arg1	microscopy					921:930	fluorescence microscopy	908:930	fluorescence microscopy	908:930	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	3	3	theme	crystallization	474:488	arg1	dynamics					490:497	the crystallization dynamics	470:497	the crystallization dynamics	470:497	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	0	4	theme	Seaweed	100:106	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	4	5	theme	crystals	721:728	arg1	charge					706:711	surface charge	698:711	surface charge	698:711	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	4	5	theme	crystals	721:728	arg1	morphology					677:686	morphology	677:686	morphology	677:686	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	4	5	theme	crystals	721:728	arg1	size					689:692	size	689:692	size	689:692	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	8	6	theme	main	1273:1276	arg1	SPs					1170:1172	SPs	1170:1172	SPs	1170:1172	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	6	theme	main	1273:1276	arg1	crystals					1249:1256	CaOx crystals	1244:1256	CaOx crystals	1244:1256	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	6	theme	main	1273:1276	arg1	cause					1278:1282	the main cause	1269:1282	the main cause of kidney stones	1269:1299	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	3	7	theme	sulfated	377:384	arg1	polysaccharides					386:400	seaweeds' sulfated polysaccharides	367:400	seaweeds' sulfated polysaccharides (SPs)	367:406	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	3	7	theme	sulfated	377:384	arg1	SPs					403:405	SPs	403:405	SPs	403:405	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	0	8	theme	Green	94:98	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	6	9	theme	CaOx	836:839	arg1	crystals					841:848	dihydrate CaOx crystals	826:848	dihydrate CaOx crystals	826:848	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	7	10	theme	action	1042:1047	arg1	mode					1034:1037	the mode	1030:1037	the mode of action between the Caulerpa's SPs and the CaOx crystals	1030:1096	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	5	11	located	found	776:780	arg1	persons					793:799	healthy persons	785:799	healthy persons	785:799	Thus, these crystals became similar to those found in healthy persons.					
31159355	5	11	located	found	776:780	arg2	those					770:774	those	770:774	those	770:774	Thus, these crystals became similar to those found in healthy persons.					
31159355	4	12	theme	CaOx	716:719	arg1	crystals					721:728	CaOx crystals	716:728	CaOx crystals	716:728	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	0	13	theme	cupressoides	117:128	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	6	14	theme	dihydrate	826:834	arg1	crystals					841:848	dihydrate CaOx crystals	826:848	dihydrate CaOx crystals	826:848	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	4	15	theme	surface	698:704	arg1	charge					706:711	surface charge	698:711	surface charge	698:711	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	0	16	theme	Caulerpa	108:115	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	0	17	from	Effect	38:43	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	6	18	dep	SPs	821:823	arg1	the					805:807	the	805:807	the	805:807	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	6	18	dep	SPs	821:823	arg1	presence					809:816	presence	809:816	presence	809:816	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	7	19	theme	Infrared	889:896	arg1	analysis					898:905	Infrared analysis	889:905	Infrared analysis	889:905	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	3	20	theme	negative	530:537	arg1	charges					539:545	the negative charges	526:545	the negative charges of these polymers	526:563	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	1	21	theme	world	192:196	arg1	population					198:207	the world population	188:207	the world population	188:207	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	0	22	theme	In	0:1	arg1	Studies					9:15	In Vitro Studies	0:15	In Vitro Studies	0:15	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	2	23	used	used	328:331	arg2	compound					307:314	no efficient compound	294:314	no efficient compound that can be used to prevent this disease	294:355	Currently, there is no efficient compound that can be used to prevent this disease.					
31159355	3	24	theme	charges	539:545	arg1	interaction					511:521	the interaction	507:521	the interaction of the negative charges of these polymers with the crystal surface during their synthesis	507:611	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	1	25	theme	population	198:207	arg1	population					198:207	the world population	188:207	the world population	188:207	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	1	25	theme	population	198:207	arg1	%					183:183	approximately 10%	167:183	approximately 10% of the world population	167:207	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	2	26	theme	efficient	297:305	arg1	compound					307:314	no efficient compound	294:314	no efficient compound that can be used to prevent this disease	294:355	Currently, there is no efficient compound that can be used to prevent this disease.					
31159355	8	27	theme	kidney	1287:1292	arg1	stones					1294:1299	kidney stones	1287:1299	kidney stones	1287:1299	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	7	28	theme	CaOx	1084:1087	arg1	crystals					1089:1096	the CaOx crystals	1080:1096	the CaOx crystals	1080:1096	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	0	29	theme	var	130:132	arg1	flabellata					134:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	the Green Seaweed Caulerpa cupressoides var flabellata	90:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	4	30	theme	cupressoides	651:662	arg1	SPs					635:637	the SPs	631:637	the SPs of Caulerpa cupressoides	631:662	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	5	31	theme	healthy	785:791	arg1	persons					793:799	healthy persons	785:799	healthy persons	785:799	Thus, these crystals became similar to those found in healthy persons.					
31159355	8	32	theme	promising	1185:1193	arg1	molecules					1195:1203	promising molecules	1185:1203	promising molecules for the treatment of urolithiasis	1185:1237	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	32	theme	promising	1185:1193	arg1	SPs					1170:1172	SPs	1170:1172	SPs	1170:1172	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	32	theme	promising	1185:1193	arg1	crystals					1249:1256	CaOx crystals	1244:1256	CaOx crystals	1244:1256	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	3	33	theme	polymers	556:563	arg1	charges					539:545	the negative charges	526:545	the negative charges of these polymers	526:563	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	3	34	theme	CaOx	423:426	arg1	crystals					428:435	the CaOx crystals surface's charge	419:452	the CaOx crystals surface's charge	419:452	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	0	35	theme	Antiurolithic	24:36	arg1	Effect					38:43	Antiurolithic Effect	24:43	Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	24:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	8	36	theme	first	1117:1121	arg1	step					1123:1126	the first step	1113:1126	the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones	1113:1299	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	36	theme	first	1117:1121	arg1	study					1104:1108	This study	1099:1108	This study	1099:1108	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	0	37	from	flabellata	134:143	arg1	Effect					38:43	Antiurolithic Effect	24:43	Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	24:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	0	37	from	flabellata	134:143	arg1	Polysaccharides					69:83	Antioxidant Sulfated Polysaccharides	48:83	Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	48:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	6	38	theme	dumbbell	868:875	arg1	morphology					877:886	rounded or dumbbell morphology	857:886	rounded or dumbbell morphology	857:886	In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology.					
31159355	0	39	theme	Antioxidant	48:58	arg1	Polysaccharides					69:83	Antioxidant Sulfated Polysaccharides	48:83	Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	48:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	3	40	with	interaction	511:521	arg1	surface					582:588	the crystal surface	570:588	the crystal surface during their synthesis	570:611	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
31159355	8	41	theme	stones	1294:1299	arg1	SPs					1170:1172	SPs	1170:1172	SPs	1170:1172	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	41	theme	stones	1294:1299	arg1	crystals					1249:1256	CaOx crystals	1244:1256	CaOx crystals	1244:1256	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	41	theme	stones	1294:1299	arg1	cause					1278:1282	the main cause	1269:1282	the main cause of kidney stones	1269:1299	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	7	42	theme	FITC-conjugated	949:963	arg1	cytometry					938:946	flow cytometry	933:946	flow cytometry (FITC-conjugated SPs)	933:968	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	7	42	theme	FITC-conjugated	949:963	arg1	SPs					965:967	FITC-conjugated SPs	949:967	FITC-conjugated SPs	949:967	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	0	43	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	7	44	theme	atomic	974:979	arg1	analysis					993:1000	atomic composition analysis	974:1000	atomic composition analysis (EDS)	974:1006	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	7	44	theme	atomic	974:979	arg1	EDS					1003:1005	EDS	1003:1005	EDS	1003:1005	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	4	45	dep	morphology	677:686	arg1	the					673:675	the	673:675	the	673:675	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	7	46	theme	composition	981:991	arg1	analysis					993:1000	atomic composition analysis	974:1000	atomic composition analysis (EDS)	974:1006	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	7	46	theme	composition	981:991	arg1	EDS					1003:1005	EDS	1003:1005	EDS	1003:1005	Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa's SPs and the CaOx crystals.					
31159355	8	47	theme	urolithiasis	1226:1237	arg1	treatment					1213:1221	the treatment	1209:1221	the treatment of urolithiasis	1209:1237	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	48	theme	CaOx	1244:1247	arg1	molecules					1195:1203	promising molecules	1185:1203	promising molecules for the treatment of urolithiasis	1185:1237	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	48	theme	CaOx	1244:1247	arg1	SPs					1170:1172	SPs	1170:1172	SPs	1170:1172	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	48	theme	CaOx	1244:1247	arg1	crystals					1249:1256	CaOx crystals	1244:1256	CaOx crystals	1244:1256	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	8	48	theme	CaOx	1244:1247	arg1	cause					1278:1282	the main cause	1269:1282	the main cause of kidney stones	1269:1299	This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.					
31159355	1	49	theme	calcium	241:247	arg1	crystals					264:271	calcium oxalate (CaOx) crystals	241:271	calcium oxalate (CaOx) crystals	241:271	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	1	49	theme	calcium	241:247	arg1	CaOx					258:261	CaOx	258:261	CaOx	258:261	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	0	50	theme	Polysaccharides	69:83	arg1	Effect					38:43	Antiurolithic Effect	24:43	Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	24:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	4	51	theme	Caulerpa	642:649	arg1	cupressoides					651:662	Caulerpa cupressoides	642:662	Caulerpa cupressoides	642:662	We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals.					
31159355	1	52	theme	oxalate	249:255	arg1	crystals					264:271	calcium oxalate (CaOx) crystals	241:271	calcium oxalate (CaOx) crystals	241:271	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	1	52	theme	oxalate	249:255	arg1	CaOx					258:261	CaOx	258:261	CaOx	258:261	Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals.					
31159355	0	53	theme	Sulfated	60:67	arg1	Polysaccharides					69:83	Antioxidant Sulfated Polysaccharides	48:83	Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata	48:143	In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata.					
31159355	3	54	theme	crystal	574:580	arg1	surface					582:588	the crystal surface	570:588	the crystal surface during their synthesis	570:611	However, seaweeds' sulfated polysaccharides (SPs) can change the CaOx crystals surface's charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis.					
29452183	3	0	contain	have	542:545	arg2	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	3	0	contain	have	542:545	arg1	AMPS-III					506:513	AMPS-III	506:513	AMPS-III	506:513	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	5	1	theme	food	1001:1004	arg1	supplements					1006:1016	anti-inflammatory food supplements	983:1016	anti-inflammatory food supplements	983:1016	The results provide helpful evidences for application of AMPS-III as anti-inflammatory food supplements.					
29452183	0	2	theme	galactofucan	80:91	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	0	2	theme	galactofucan	80:91	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	0	2	theme	galactofucan	80:91	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity	42:67	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	3	3	link	1,3-linked	573:582	arg1	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	4	4	theme	factor-α	774:781	arg1	release					748:754	the release	744:754	the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages	744:865	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	2	5	theme	AMPS-III	302:309	arg1	structure					371:379	structure	371:379	structure	371:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	5	theme	AMPS-III	302:309	arg1	structure					289:297	chemical structure	280:297	chemical structure of AMPS-III including the linkages of compositional monosaccharides	280:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	5	theme	AMPS-III	302:309	arg1	characteristics					264:278	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure	260:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit	260:398	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	0	6	theme	Armillaria	98:107	arg1	mellea					109:114	Armillaria mellea	98:114	Armillaria mellea	98:114	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	4	7	theme	monocyte	804:811	arg1	MCP-1					836:840	MCP-1	836:840	MCP-1	836:840	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	7	theme	monocyte	804:811	arg1	protein-1					825:833	cytokine monocyte chemotactic protein-1	795:833	cytokine monocyte chemotactic protein-1 (MCP-1)	795:841	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	8	theme	cytokine	795:802	arg1	MCP-1					836:840	MCP-1	836:840	MCP-1	836:840	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	8	theme	cytokine	795:802	arg1	protein-1					825:833	cytokine monocyte chemotactic protein-1	795:833	cytokine monocyte chemotactic protein-1 (MCP-1)	795:841	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	3	9	theme	1,3-linked	573:582	arg1	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	4	10	theme	necrosis	765:772	arg1	TNF-α					784:788	TNF-α	784:788	TNF-α	784:788	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	10	theme	necrosis	765:772	arg1	factor-α					774:781	tumor necrosis factor-α	759:781	tumor necrosis factor-α (TNF-α)	759:789	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	0	11	theme	anti-inflammatory	42:58	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity	42:67	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	2	12	theme	chemical	280:287	arg1	structure					289:297	chemical structure	280:297	chemical structure of AMPS-III including the linkages of compositional monosaccharides	280:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	12	theme	chemical	280:287	arg1	characteristics					264:278	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure	260:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit	260:398	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	13	theme	magnetic	475:482	arg1	resonance					484:492	nuclear magnetic resonance	467:492	two-dimensional nuclear magnetic resonance techniques	451:503	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	3	14	theme	4-O-xylosylated	557:571	arg1	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	3	15	theme	pentadecasaccharide	634:652	arg1	unit					661:664	a pentadecasaccharide repeat unit	632:664	a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	632:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	4	16	from	release	748:754	arg1	macrophages					855:865	RAW264.7 macrophages	846:865	RAW264.7 macrophages	846:865	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	2	17	dep	characteristics	264:278	arg1	structure					371:379	structure	371:379	structure	371:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	17	dep	characteristics	264:278	arg1	structure					289:297	chemical structure	280:297	chemical structure of AMPS-III including the linkages of compositional monosaccharides	280:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	17	dep	characteristics	264:278	arg1	characteristics					264:278	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure	260:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit	260:398	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	18	theme	nuclear	467:473	arg1	resonance					484:492	nuclear magnetic resonance	467:492	two-dimensional nuclear magnetic resonance techniques	451:503	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	3	19	theme	repeat	654:659	arg1	unit					661:664	a pentadecasaccharide repeat unit	632:664	a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	632:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	1	20	theme	novel	188:192	arg1	agent					212:216	a novel anti-inflammatory agent	186:216	a novel anti-inflammatory agent from an edible fungus, Armillaria mellea	186:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	20	theme	novel	188:192	arg1	1,3-galactofucan					127:142	A xylosyl 1,3-galactofucan	117:142	A xylosyl 1,3-galactofucan (AMPS-III)	117:153	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	2	21	theme	two-dimensional	451:465	arg1	techniques					494:503	two-dimensional nuclear magnetic resonance techniques	451:503	two-dimensional nuclear magnetic resonance techniques	451:503	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	4	22	theme	protein-1	825:833	arg1	release					748:754	the release	744:754	the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages	744:865	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	1	23	theme	anti-inflammatory	194:210	arg1	agent					212:216	a novel anti-inflammatory agent	186:216	a novel anti-inflammatory agent from an edible fungus, Armillaria mellea	186:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	23	theme	anti-inflammatory	194:210	arg1	1,3-galactofucan					127:142	A xylosyl 1,3-galactofucan	117:142	A xylosyl 1,3-galactofucan (AMPS-III)	117:153	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	0	24	theme	structural	14:23	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	3	25	theme	partial	549:555	arg1	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	3	26	theme	α-d-galactosyl-interlaced	584:608	arg1	α-l-fucan					610:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan	547:618	a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa	547:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	4	27	theme	tumor	759:763	arg1	TNF-α					784:788	TNF-α	784:788	TNF-α	784:788	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	27	theme	tumor	759:763	arg1	factor-α					774:781	tumor necrosis factor-α	759:781	tumor necrosis factor-α (TNF-α)	759:789	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	5	28	theme	helpful	934:940	arg1	evidences					942:950	helpful evidences	934:950	helpful evidences for application of AMPS-III as anti-inflammatory food supplements	934:1016	The results provide helpful evidences for application of AMPS-III as anti-inflammatory food supplements.					
29452183	3	29	theme	molecular	673:681	arg1	mass					683:686	a molecular mass	671:686	a molecular mass approximately 13 kDa	671:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	3	30	with	unit	661:664	arg1	mass					683:686	a molecular mass	671:686	a molecular mass approximately 13 kDa	671:707	AMPS-III was chemically proposed to have a partial 4-O-xylosylated 1,3-linked α-d-galactosyl-interlaced α-l-fucan composed of a pentadecasaccharide repeat unit with a molecular mass approximately 13 kDa.					
29452183	1	31	theme	xylosyl	119:125	arg1	agent					212:216	a novel anti-inflammatory agent	186:216	a novel anti-inflammatory agent from an edible fungus, Armillaria mellea	186:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	31	theme	xylosyl	119:125	arg1	AMPS-III					145:152	AMPS-III	145:152	AMPS-III	145:152	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	31	theme	xylosyl	119:125	arg1	1,3-galactofucan					127:142	A xylosyl 1,3-galactofucan	117:142	A xylosyl 1,3-galactofucan (AMPS-III)	117:153	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	2	32	theme	resonance	484:492	arg1	techniques					494:503	two-dimensional nuclear magnetic resonance techniques	451:503	two-dimensional nuclear magnetic resonance techniques	451:503	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	4	33	theme	TNF-α	897:901	arg1	induction					903:911	LPS and TNF-α induction	889:911	induction	903:911	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	2	34	theme	monosaccharides	351:365	arg1	linkages					325:332	the linkages	321:332	the linkages of compositional monosaccharides	321:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	5	35	theme	AMPS-III	971:978	arg1	application					956:966	application	956:966	application of AMPS-III as anti-inflammatory food supplements	956:1016	The results provide helpful evidences for application of AMPS-III as anti-inflammatory food supplements.					
29452183	1	36	theme	edible	226:231	arg1	fungus					233:238	an edible fungus	223:238	an edible fungus	223:238	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	36	theme	edible	226:231	arg1	mellea					252:257	Armillaria mellea	241:257	Armillaria mellea	241:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	37	from	fungus	233:238	arg1	agent					212:216	a novel anti-inflammatory agent	186:216	a novel anti-inflammatory agent from an edible fungus, Armillaria mellea	186:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	37	from	fungus	233:238	arg1	1,3-galactofucan					127:142	A xylosyl 1,3-galactofucan	117:142	A xylosyl 1,3-galactofucan (AMPS-III)	117:153	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	0	38	from	mellea	109:114	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	0	38	from	mellea	109:114	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	0	38	from	mellea	109:114	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity	42:67	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	4	39	theme	RAW264.7	846:853	arg1	macrophages					855:865	RAW264.7 macrophages	846:865	RAW264.7 macrophages	846:865	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	2	40	theme	compositional	337:349	arg1	monosaccharides					351:365	compositional monosaccharides	337:365	compositional monosaccharides	337:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	4	41	theme	chemotactic	813:823	arg1	MCP-1					836:840	MCP-1	836:840	MCP-1	836:840	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	4	41	theme	chemotactic	813:823	arg1	protein-1					825:833	cytokine monocyte chemotactic protein-1	795:833	cytokine monocyte chemotactic protein-1 (MCP-1)	795:841	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	0	42	theme	xylosyl	72:78	arg1	galactofucan					80:91	xylosyl galactofucan	72:91	xylosyl galactofucan	72:91	Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea.					
29452183	5	43	theme	anti-inflammatory	983:999	arg1	supplements					1006:1016	anti-inflammatory food supplements	983:1016	anti-inflammatory food supplements	983:1016	The results provide helpful evidences for application of AMPS-III as anti-inflammatory food supplements.					
29452183	2	44	theme	unit	395:398	arg1	structure					371:379	structure	371:379	structure	371:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	44	theme	unit	395:398	arg1	structure					289:297	chemical structure	280:297	chemical structure of AMPS-III including the linkages of compositional monosaccharides	280:365	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	2	44	theme	unit	395:398	arg1	characteristics					264:278	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure	260:379	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit	260:398	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	4	45	theme	LPS	889:891	arg1	induction					903:911	LPS and TNF-α induction	889:911	induction	903:911	AMPS-III significantly suppressed the release of tumor necrosis factor-α (TNF-α) and cytokine monocyte chemotactic protein-1 (MCP-1) in RAW264.7 macrophages and EAhy926 following LPS and TNF-α induction.					
29452183	2	46	theme	repeat	388:393	arg1	unit					395:398	the repeat unit	384:398	the repeat unit	384:398	The characteristics chemical structure of AMPS-III including the linkages of compositional monosaccharides and structure of the repeat unit were depicted and elucidated by proton, carbon and two-dimensional nuclear magnetic resonance techniques.					
29452183	1	47	theme	Armillaria	241:250	arg1	fungus					233:238	an edible fungus	223:238	an edible fungus	223:238	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29452183	1	47	theme	Armillaria	241:250	arg1	mellea					252:257	Armillaria mellea	241:257	Armillaria mellea	241:257	A xylosyl 1,3-galactofucan (AMPS-III) was isolated and identified as a novel anti-inflammatory agent from an edible fungus, Armillaria mellea.					
29414729	0	0	theme	longibrachiatum	93:107	arg1	exochitinase					118:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase	68:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides	68:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	1	1	theme	Trichoderma	375:385	arg1	exochitinase					412:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	7	2	theme	higher	1562:1567	arg1	effect					1580:1585	higher antifungal effect	1562:1585	higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger	1562:1657	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	5	3	theme	denaturation	1194:1205	arg1	6.88 Kcal·mol-1					1212:1226	denaturation (Ed) 6.88 Kcal·mol-1	1194:1226	denaturation (Ed) 6.88 Kcal·mol-1	1194:1226	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	5	4	theme	activation	1134:1143	arg1	energy					1145:1150	activation energy	1134:1150	higher activation energy (Ea) 3.39 Kcal·mol-1	1127:1171	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	5	4	theme	activation	1134:1143	arg1	Ea					1153:1154	Ea	1153:1154	Ea	1153:1154	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	5	5	theme	conjugated	1284:1293	arg1	form					1295:1298	conjugated form	1284:1298	conjugated form	1284:1298	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	0	6	theme	Trichoderma	81:91	arg1	exochitinase					118:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase	68:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides	68:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	1	7	theme	longibrachiatum	387:401	arg1	exochitinase					412:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	5	8	theme	energy	1145:1150	arg1	3.39 Kcal·mol-1					1157:1171	higher activation energy (Ea) 3.39 Kcal·mol-1	1127:1171	higher activation energy (Ea) 3.39 Kcal·mol-1	1127:1171	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	2	9	theme	chemical	537:544	arg1	modification					546:557	the chemical modification	533:557	the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase	533:610	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	0	10	theme	exochitinase	118:129	arg1	Improvement					0:10	Improvement	0:10	Improvement of catalytic	0:23	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	0	10	theme	exochitinase	118:129	arg1	thermodynamics					26:39	thermodynamics	26:39	thermodynamics	26:39	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	0	10	theme	exochitinase	118:129	arg1	activity					56:63	antifungal activity	45:63	antifungal activity	45:63	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	1	11	theme	KT693225	403:410	arg1	exochitinase					412:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	6	12	theme	conjugated	1371:1380	arg1	exochitinase					1382:1393	native and conjugated exochitinase	1360:1393	native and conjugated exochitinase	1360:1393	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	6	13	theme	parameters	1329:1338	arg1	values					1305:1310	The values	1301:1310	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase	1301:1393	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	0	14	theme	KT693225	109:116	arg1	exochitinase					118:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase	68:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides	68:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	1	15	theme	exochitinase	412:423	arg1	thermodynamics					348:361	the thermodynamics	344:361	the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase	344:423	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	4	16	theme	native	946:951	arg1	form					953:956	native form	946:956	native form	946:956	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	3	17	from	55	805:806	arg1	increase					769:776	4.32, 2.75 and 2.44-fold increase	744:776	increase	769:776	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	17	from	55	805:806	arg1	values					791:796	half-life values	781:796	half-life values at 50, 55 and 60 °C, respectively	781:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	0	18	theme	covalent	134:141	arg1	coupling					143:150	covalent coupling	134:150	covalent coupling to oxidized polysaccharides	134:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	7	19	theme	antifungal	1569:1578	arg1	effect					1580:1585	higher antifungal effect	1562:1585	higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger	1562:1657	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	6	20	theme	native	1360:1365	arg1	exochitinase					1382:1393	native and conjugated exochitinase	1360:1393	native and conjugated exochitinase	1360:1393	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	4	21	theme	conjugated	839:848	arg1	exochitinase					850:861	conjugated exochitinase	839:861	conjugated exochitinase	839:861	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	2	22	theme	Trichoderma	562:572	arg1	exochitinase					599:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	1	23	theme	covalent	428:435	arg1	coupling					437:444	covalent coupling	428:444	covalent coupling to sodium periodate activated agar	428:479	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	3	24	from	60 °C	812:816	arg1	increase					769:776	4.32, 2.75 and 2.44-fold increase	744:776	increase	769:776	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	24	from	60 °C	812:816	arg1	values					791:796	half-life values	781:796	half-life values at 50, 55 and 60 °C, respectively	781:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	25	theme	Optimum	613:619	arg1	temperature					621:631	Optimum temperature	613:631	Optimum temperature for conjugated exochitinase 60 °C	613:665	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	4	26	theme	lower	998:1002	arg1	60 °C					1056:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	2	27	theme	longibrachiatum	574:588	arg1	exochitinase					599:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	3	28	theme	4.32	744:747	arg1	increase					769:776	4.32, 2.75 and 2.44-fold increase	744:776	increase	769:776	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	4	29	theme	0.39 × 10-3 min-1at	1036:1054	arg1	60 °C					1056:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	7	30	theme	Alternaria	1595:1604	arg1	alternata					1606:1614	Alternaria alternata	1595:1614	Alternaria alternata	1595:1614	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	6	31	theme	free	1493:1496	arg1	ΔG°					1506:1508	ΔG°	1506:1508	ΔG°	1506:1508	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	6	31	theme	free	1493:1496	arg1	energy					1498:1503	free energy	1493:1503	free energy (ΔG°) of deactivation	1493:1525	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	4	32	theme	constant	1017:1024	arg1	rate					1026:1029	deactivation constant rate	1004:1029	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	32	theme	constant	1017:1024	arg1	kd					1032:1033	kd	1032:1033	kd	1032:1033	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	33	dep	showed	863:868	arg1	compared					919:926	compared	919:926	showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C.	863:966	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	3	34	from	50	801:802	arg1	increase					769:776	4.32, 2.75 and 2.44-fold increase	744:776	increase	769:776	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	34	from	50	801:802	arg1	values					791:796	half-life values	781:796	half-life values at 50, 55 and 60 °C, respectively	781:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	1	35	theme	agricultural	309:320	arg1	wastes					322:327	agricultural wastes	309:327	agricultural wastes	309:327	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	4	36	contain	had	994:996	arg2	60 °C					1056:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	36	contain	had	994:996	arg1	form					989:992	conjugated form	978:992	conjugated form	978:992	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	1	37	theme	main	210:213	arg1	goals					215:219	two main goals	206:219	two main goals preparation of constitutive exochitinase with low cost	206:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	6	38	theme	deactivation	1514:1525	arg1	enthalpy					1474:1481	enthalpy	1474:1481	enthalpy (ΔH°)	1474:1487	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	6	38	theme	deactivation	1514:1525	arg1	ΔG°					1506:1508	ΔG°	1506:1508	ΔG°	1506:1508	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	6	38	theme	deactivation	1514:1525	arg1	energy					1498:1503	free energy	1493:1503	free energy (ΔG°) of deactivation	1493:1525	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	6	38	theme	deactivation	1514:1525	arg1	ΔH°					1484:1486	ΔH°	1484:1486	ΔH°	1484:1486	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	4	39	theme	D-values	877:884	arg1	1790.49					911:917	higher D-values (decimal reduction time) 1790.49	870:917	higher D-values (decimal reduction time) 1790.49	870:917	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	3	40	theme	conjugated	637:646	arg1	60 °C					661:665	conjugated exochitinase 60 °C	637:665	conjugated exochitinase 60 °C	637:665	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	4	41	theme	decimal	887:893	arg1	D-values					877:884	D-values	877:884	higher D-values (decimal reduction time) 1790.49	870:917	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	41	theme	decimal	887:893	arg1	time					905:908	decimal reduction time	887:908	decimal reduction time	887:908	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	1	42	theme	goals	215:219	arg1	preparation					221:231	two main goals preparation	206:231	two main goals preparation of constitutive exochitinase with low cost	206:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	0	43	theme	catalytic	15:23	arg1	Improvement					0:10	Improvement	0:10	Improvement of catalytic	0:23	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	0	43	theme	catalytic	15:23	arg1	thermodynamics					26:39	thermodynamics	26:39	thermodynamics	26:39	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	0	43	theme	catalytic	15:23	arg1	activity					56:63	antifungal activity	45:63	antifungal activity	45:63	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	4	44	theme	conjugated	978:987	arg1	form					989:992	conjugated form	978:992	conjugated form	978:992	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	3	45	theme	exochitinase	648:659	arg1	60 °C					661:665	conjugated exochitinase 60 °C	637:665	conjugated exochitinase 60 °C	637:665	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	2	46	theme	Central	482:488	arg1	CCD					508:510	CCD	508:510	CCD	508:510	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	2	46	theme	Central	482:488	arg1	design					500:505	Central composite design	482:505	Central composite design (CCD)	482:511	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	3	47	from	increase	769:776	arg1	50					801:802	50	801:802	50	801:802	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	47	from	increase	769:776	arg1	values					791:796	half-life values	781:796	half-life values at 50, 55 and 60 °C, respectively	781:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	47	from	increase	769:776	arg1	60 °C					812:816	60 °C	812:816	60 °C	812:816	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	47	from	increase	769:776	arg1	55					805:806	55	805:806	55	805:806	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	4	48	theme	deactivation	1004:1015	arg1	rate					1026:1029	deactivation constant rate	1004:1029	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	48	theme	deactivation	1004:1015	arg1	kd					1032:1033	kd	1032:1033	kd	1032:1033	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	1	49	theme	sodium	449:454	arg1	periodate					456:464	sodium periodate	449:464	sodium periodate	449:464	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	3	50	theme	native	683:688	arg1	form					690:693	native form	683:693	native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively	683:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	3	51	dep	form	690:693	arg1	caused					737:742	caused	737:742	caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively	737:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	7	52	theme	Conjugated	1528:1537	arg1	exochitinase					1539:1550	Conjugated exochitinase	1528:1550	Conjugated exochitinase	1528:1550	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	0	53	theme	oxidized	155:162	arg1	polysaccharides					164:178	oxidized polysaccharides	155:178	oxidized polysaccharides	155:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	3	54	theme	oxidized	723:730	arg1	agar					732:735	oxidized agar	723:735	oxidized agar	723:735	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	5	55	theme	Native	1097:1102	arg1	exochitinase					1104:1115	Native exochitinase	1097:1115	Native exochitinase	1097:1115	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	1	56	theme	constitutive	236:247	arg1	exochitinase					249:260	constitutive exochitinase	236:260	constitutive exochitinase with low cost	236:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	2	57	theme	exochitinase	599:610	arg1	modification					546:557	the chemical modification	533:557	the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase	533:610	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	5	58	theme	lower	1177:1181	arg1	energy					1183:1188	lower energy	1177:1188	lower energy for denaturation (Ed) 6.88 Kcal·mol-1	1177:1226	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	1	59	theme	activated	466:474	arg1	agar					476:479	sodium periodate activated agar	449:479	sodium periodate activated agar	449:479	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	0	60	theme	antifungal	45:54	arg1	Improvement					0:10	Improvement	0:10	Improvement of catalytic	0:23	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	0	60	theme	antifungal	45:54	arg1	activity					56:63	antifungal activity	45:63	antifungal activity	45:63	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	3	61	theme	half-life	781:789	arg1	values					791:796	half-life values	781:796	half-life values at 50, 55 and 60 °C, respectively	781:830	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	5	62	theme	higher	1127:1132	arg1	3.39 Kcal·mol-1					1157:1171	higher activation energy (Ea) 3.39 Kcal·mol-1	1127:1171	higher activation energy (Ea) 3.39 Kcal·mol-1	1127:1171	Native exochitinase exhibited higher activation energy (Ea) 3.39 Kcal·mol-1 and lower energy for denaturation (Ed) 6.88 Kcal·mol-1 compared to 3.21 and 13.05 Kcal·mol-1, respectively for conjugated form.					
29414729	4	63	theme	reduction	895:903	arg1	D-values					877:884	D-values	877:884	higher D-values (decimal reduction time) 1790.49	870:917	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	4	63	theme	reduction	895:903	arg1	time					905:908	decimal reduction time	887:908	decimal reduction time	887:908	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	7	64	theme	Aspergillus	1641:1651	arg1	niger					1653:1657	Aspergillus niger	1641:1657	Aspergillus niger	1641:1657	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	7	65	theme	native	1664:1669	arg1	form					1671:1674	native form	1664:1674	native form	1664:1674	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	4	66	theme	higher	870:875	arg1	1790.49					911:917	higher D-values (decimal reduction time) 1790.49	870:917	higher D-values (decimal reduction time) 1790.49	870:917	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	6	67	theme	exochitinase	1382:1393	arg1	inactivation					1344:1355	inactivation	1344:1355	inactivation of native and conjugated exochitinase	1344:1393	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	3	68	theme	2.44-fold	759:767	arg1	increase					769:776	4.32, 2.75 and 2.44-fold increase	744:776	increase	769:776	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	1	69	contain	containing	298:307	arg1	non-chitin					287:296	non-chitin	287:296	non-chitin containing agricultural wastes	287:327	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	1	69	contain	containing	298:307	arg2	wastes					322:327	agricultural wastes	309:327	agricultural wastes	309:327	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	6	70	theme	thermodynamic	1315:1327	arg1	parameters					1329:1338	thermodynamic parameters	1315:1338	thermodynamic parameters	1315:1338	The values of thermodynamic parameters for inactivation of native and conjugated exochitinase indicated that conjugation significantly decreased entropy (ΔS°) and increased enthalpy (ΔH°) and free energy (ΔG°) of deactivation.					
29414729	1	71	theme	exochitinase	249:260	arg1	preparation					221:231	two main goals preparation	206:231	two main goals preparation of constitutive exochitinase with low cost	206:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	4	72	theme	native	1067:1072	arg1	form					1074:1077	native form 1.7 × 10-3 min-1	1067:1094	native form 1.7 × 10-3 min-1	1067:1094	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	2	73	used	used	517:520	arg2	design					500:505	Central composite design	482:505	Central composite design (CCD)	482:511	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	2	73	used	used	517:520	arg2	CCD					508:510	CCD	508:510	CCD	508:510	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	1	74	dep	activated	466:474	arg1	periodate					456:464	sodium periodate	449:464	sodium periodate	449:464	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	0	75	theme	constitutive	68:79	arg1	exochitinase					118:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase	68:129	constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides	68:178	Improvement of catalytic, thermodynamics and antifungal activity of constitutive Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to oxidized polysaccharides.					
29414729	7	76	theme	Fusarium	1617:1624	arg1	oxysporium					1626:1635	Fusarium oxysporium	1617:1635	Fusarium oxysporium	1617:1635	Conjugated exochitinase exhibited higher antifungal effect against Alternaria alternata, Fusarium oxysporium and Aspergillus niger than native form.					
29414729	3	77	theme	40 °C.	695:700	arg1	coupling					711:718	40 °C. Covalent coupling	695:718	40 °C. Covalent coupling to oxidized agar	695:735	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	2	78	theme	composite	490:498	arg1	CCD					508:510	CCD	508:510	CCD	508:510	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	2	78	theme	composite	490:498	arg1	design					500:505	Central composite design	482:505	Central composite design (CCD)	482:511	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
29414729	1	79	theme	low	267:269	arg1	cost					271:274	low cost	267:274	low cost	267:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	1	80	theme	purified	366:373	arg1	exochitinase					412:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	purified Trichoderma longibrachiatum KT693225 exochitinase	366:423	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	3	81	theme	Covalent	702:709	arg1	coupling					711:718	40 °C. Covalent coupling	695:718	40 °C. Covalent coupling to oxidized agar	695:735	Optimum temperature for conjugated exochitinase 60 °C was higher than native form 40 °C. Covalent coupling to oxidized agar caused 4.32, 2.75 and 2.44-fold increase in half-life values at 50, 55 and 60 °C, respectively.					
29414729	1	82	with	exochitinase	249:260	arg1	cost					271:274	low cost	267:274	low cost	267:274	Our study full filled in two main goals preparation of constitutive exochitinase with low cost, utilizing non-chitin containing agricultural wastes, and improving the thermodynamics of purified Trichoderma longibrachiatum KT693225 exochitinase by covalent coupling to sodium periodate activated agar.					
29414729	4	83	theme	rate	1026:1029	arg1	60 °C					1056:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C	998:1060	Also, conjugated exochitinase showed higher D-values (decimal reduction time) 1790.49 compared to 733.08 min for native form at 60 °C. Moreover, conjugated form had lower deactivation constant rate (kd) 0.39 × 10-3 min-1at 60 °C than native form 1.7 × 10-3 min-1.					
29414729	2	84	theme	KT693225	590:597	arg1	exochitinase					599:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Trichoderma longibrachiatum KT693225 exochitinase	562:610	Central composite design (CCD) was used to improve the chemical modification of Trichoderma longibrachiatum KT693225 exochitinase.					
31209074	3	0	theme	cell	345:348	arg1	poles					350:354	its cell poles	341:354	its cell poles	341:354	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	7	1	contain	contains	900:907	arg1	holdfast					891:898	the holdfast	887:898	the holdfast	887:898	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	7	1	contain	contains	900:907	arg2	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	12	2	theme	holdfast	1934:1941	arg1	attachment					1943:1952	holdfast attachment	1934:1952	holdfast attachment	1934:1952	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	7	3	theme	1,4-linked	911:920	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	9	4	theme	carbohydrate-based	1479:1496	arg1	adhesin					1498:1504	a carbohydrate-based adhesin	1477:1504	a carbohydrate-based adhesin called the holdfast	1477:1524	Caulobacter crescentus initiates surface colonization by secreting a carbohydrate-based adhesin called the holdfast.					
31209074	3	5	theme	poles	350:354	arg1	one					334:336	one	334:336	one	334:336	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	3	5	theme	poles	350:354	arg1	poles					350:354	its cell poles	341:354	its cell poles	341:354	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	4	6	theme	adhesin	545:551	arg1	quantities					527:536	analytical quantities	516:536	analytical quantities of the adhesin	516:551	The genes required for holdfast biosynthesis have been analyzed in detail, but difficulties in isolating analytical quantities of the adhesin have limited efforts to characterize its chemical structure.					
31209074	7	7	link	1,4-linked	911:920	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	5	8	theme	content	744:750	arg1	survey					717:722	a survey	715:722	a survey of its carbohydrate content	715:750	In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content.					
31209074	5	9	theme	C.	680:681	arg1	cultures					694:701	C. crescentus cultures	680:701	C. crescentus cultures	680:701	In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content.					
31209074	12	10	theme	composition	1861:1871	arg1	analysis					1873:1880	The composition analysis	1857:1880	The composition analysis	1857:1880	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	0	11	from	crescentus	60:69	arg1	Composition					0:10	Composition	0:10	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.	0:70	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.					
31209074	5	12	theme	crescentus	683:692	arg1	cultures					694:701	C. crescentus cultures	680:701	C. crescentus cultures	680:701	In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content.					
31209074	4	13	theme	holdfast	434:441	arg1	biosynthesis					443:454	holdfast biosynthesis	434:454	holdfast biosynthesis	434:454	The genes required for holdfast biosynthesis have been analyzed in detail, but difficulties in isolating analytical quantities of the adhesin have limited efforts to characterize its chemical structure.					
31209074	1	14	theme	Surface	72:78	arg1	colonization					80:91	Surface colonization	72:91	Surface colonization	72:91	Surface colonization is central to the lifestyles of many bacteria.					
31209074	8	15	theme	holdfast	1193:1200	arg1	pathway					1202:1208	the holdfast pathway	1189:1208	the holdfast pathway	1189:1208	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	3	16	theme	polysaccharide-based	280:299	arg1	adhesin					301:307	a polysaccharide-based adhesin	278:307	a polysaccharide-based adhesin	278:307	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	7	17	theme	xylose	977:982	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	3	18	theme	tight	371:375	arg1	attachment					377:386	tight attachment	371:386	tight attachment to exogenous surfaces	371:408	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	2	19	theme	surface	151:157	arg1	niches					159:164	surface niches	151:164	surface niches	151:164	Exploiting surface niches requires sophisticated systems for sensing and attaching to solid materials.					
31209074	7	20	theme	N-acetylglucosamine	952:970	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	11	21	theme	adhesive	1840:1847	arg1	matrix					1849:1854	the adhesive matrix	1836:1854	the adhesive matrix	1836:1854	This study outlines a method to extract the C. crescentus holdfast and describes the monosaccharide components contained within the adhesive matrix.					
31209074	8	22	theme	polysaccharide	1254:1267	arg1	adhesins					1269:1276	polysaccharide adhesins	1254:1276	polysaccharide adhesins	1254:1276	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	8	23	theme	monosaccharide	1077:1090	arg1	components					1092:1101	the monosaccharide components	1073:1101	the monosaccharide components in the polysaccharide	1073:1123	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	10	24	theme	unique	1658:1663	arg1	properties					1674:1683	its unique adhesive properties	1654:1683	its unique adhesive properties	1654:1683	Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood.					
31209074	10	25	theme	adhesive	1665:1672	arg1	properties					1674:1683	its unique adhesive properties	1654:1683	its unique adhesive properties	1654:1683	Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood.					
31209074	8	26	from	enzymes	1178:1184	arg1	pathway					1202:1208	the holdfast pathway	1189:1208	the holdfast pathway	1189:1208	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	7	27	theme	mannose	943:949	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	11	28	theme	monosaccharide	1793:1806	arg1	components					1808:1817	the monosaccharide components	1789:1817	the monosaccharide components contained within the adhesive matrix	1789:1854	This study outlines a method to extract the C. crescentus holdfast and describes the monosaccharide components contained within the adhesive matrix.					
31209074	3	29	theme	exogenous	391:399	arg1	surfaces					401:408	exogenous surfaces	391:408	exogenous surfaces	391:408	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	10	30	theme	chemical	1561:1568	arg1	makeup					1570:1575	the chemical makeup	1557:1575	the chemical makeup of the holdfast	1557:1591	Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood.					
31209074	12	31	theme	missing	1967:1973	arg1	information					1975:1985	missing information	1967:1985	missing information needed to characterize enzymes in the biosynthetic pathway	1967:2044	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	12	32	theme	chemical	1915:1922	arg1	basis					1924:1928	the chemical basis	1911:1928	the chemical basis for holdfast attachment	1911:1952	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	10	33	theme	physical	1635:1642	arg1	basis					1644:1648	the physical basis	1631:1648	the physical basis for its unique adhesive properties	1631:1683	Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood.					
31209074	4	34	theme	chemical	594:601	arg1	structure					603:611	its chemical structure	590:611	its chemical structure	590:611	The genes required for holdfast biosynthesis have been analyzed in detail, but difficulties in isolating analytical quantities of the adhesin have limited efforts to characterize its chemical structure.					
31209074	7	35	from	positions	1027:1035	arg1	branches					1007:1014	branches	1007:1014	branches at the C-6 positions of glucose and mannose	1007:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	0	36	theme	Polysaccharide	28:41	arg1	Composition					0:10	Composition	0:10	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.	0:70	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.					
31209074	5	37	theme	carbohydrate	731:742	arg1	content					744:750	its carbohydrate content	727:750	its carbohydrate content	727:750	In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content.					
31209074	11	38	theme	C.	1752:1753	arg1	holdfast					1766:1773	the C. crescentus holdfast	1748:1773	the C. crescentus holdfast	1748:1773	This study outlines a method to extract the C. crescentus holdfast and describes the monosaccharide components contained within the adhesive matrix.					
31209074	0	39	theme	Holdfast	19:26	arg1	Polysaccharide					28:41	the Holdfast Polysaccharide	15:41	the Holdfast Polysaccharide	15:41	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.					
31209074	6	40	located	detected	827:834	arg1	extracts					843:850	our extracts	839:850	our extracts	839:850	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	6	40	located	detected	827:834	arg2	Glucose					753:759	Glucose	753:759	Glucose	753:759	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	6	40	located	detected	827:834	arg2	N-acetylglucosamine					790:808	N-acetylglucosamine	790:808	N-acetylglucosamine	790:808	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	6	40	located	detected	827:834	arg2	xylose					815:820	xylose	815:820	xylose	815:820	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	6	40	located	detected	827:834	arg2	3-O-methylglucose					762:778	3-O-methylglucose	762:778	3-O-methylglucose	762:778	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	6	40	located	detected	827:834	arg2	mannose					781:787	mannose	781:787	mannose	781:787	Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine, and xylose were detected in our extracts.					
31209074	7	41	theme	mannose	1052:1058	arg1	positions					1027:1035	the C-6 positions	1019:1035	the C-6 positions of glucose and mannose	1019:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	2	42	theme	sophisticated	175:187	arg1	systems					189:195	sophisticated systems	175:195	sophisticated systems	175:195	Exploiting surface niches requires sophisticated systems for sensing and attaching to solid materials.					
31209074	4	43	theme	analytical	516:525	arg1	quantities					527:536	analytical quantities	516:536	analytical quantities of the adhesin	516:551	The genes required for holdfast biosynthesis have been analyzed in detail, but difficulties in isolating analytical quantities of the adhesin have limited efforts to characterize its chemical structure.					
31209074	0	44	theme	Caulobacter	48:58	arg1	crescentus					60:69	Caulobacter crescentus	48:69	Caulobacter crescentus	48:69	Composition of the Holdfast Polysaccharide from Caulobacter crescentus.					
31209074	12	45	theme	basis	1924:1928	arg1	understanding					1894:1906	our understanding	1890:1906	our understanding of the chemical basis for holdfast attachment	1890:1952	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	2	46	theme	solid	226:230	arg1	materials					232:240	solid materials	226:240	solid materials	226:240	Exploiting surface niches requires sophisticated systems for sensing and attaching to solid materials.					
31209074	8	47	theme	broader	1225:1231	arg1	understanding					1233:1245	a broader understanding	1223:1245	a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates	1223:1326	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	1	48	theme	many	125:128	arg1	bacteria					130:137	many bacteria	125:137	many bacteria	125:137	Surface colonization is central to the lifestyles of many bacteria.					
31209074	7	49	theme	glucose	934:940	arg1	backbone					922:929	a 1,4-linked backbone	909:929	a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose	909:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	9	50	theme	surface	1443:1449	arg1	colonization					1451:1462	surface colonization	1443:1462	surface colonization	1443:1462	Caulobacter crescentus initiates surface colonization by secreting a carbohydrate-based adhesin called the holdfast.					
31209074	7	51	theme	glucose	1040:1046	arg1	positions					1027:1035	the C-6 positions	1019:1035	the C-6 positions of glucose and mannose	1019:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	7	52	theme	C-6	1023:1025	arg1	positions					1027:1035	the C-6 positions	1019:1035	the C-6 positions of glucose and mannose	1019:1058	Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine, and xylose that is decorated with branches at the C-6 positions of glucose and mannose.					
31209074	1	53	theme	bacteria	130:137	arg1	lifestyles					111:120	the lifestyles	107:120	the lifestyles of many bacteria	107:137	Surface colonization is central to the lifestyles of many bacteria.					
31209074	10	54	theme	holdfast	1584:1591	arg1	makeup					1570:1575	the chemical makeup	1557:1575	the chemical makeup of the holdfast	1557:1591	Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood.					
31209074	12	55	theme	biosynthetic	2025:2036	arg1	pathway					2038:2044	the biosynthetic pathway	2021:2044	the biosynthetic pathway	2021:2044	The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.					
31209074	3	56	from	one	334:336	arg1	holdfast					322:329	the holdfast	318:329	the holdfast at one of its cell poles	318:354	Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces.					
31209074	8	57	theme	solid	1311:1315	arg1	substrates					1317:1326	solid substrates	1311:1326	solid substrates	1311:1326	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	5	58	from	cultures	694:701	arg1	extract					654:660	extract	654:660	extract	654:660	In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content.					
31209074	8	59	theme	dedicated	1351:1359	arg1	adhesins					1361:1368	dedicated adhesins	1351:1368	dedicated adhesins that facilitate attachment to surfaces	1351:1407	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
31209074	11	60	theme	crescentus	1755:1764	arg1	holdfast					1766:1773	the C. crescentus holdfast	1748:1773	the C. crescentus holdfast	1748:1773	This study outlines a method to extract the C. crescentus holdfast and describes the monosaccharide components contained within the adhesive matrix.					
31209074	8	61	from	components	1092:1101	arg1	polysaccharide					1110:1123	the polysaccharide	1106:1123	the polysaccharide	1106:1123	By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.IMPORTANCE To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces.					
29110612	9	0	with	variation	1063:1071	arg1	composition					1119:1129	composition	1119:1129	composition	1119:1129	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	5	1	theme	Raman	702:706	arg1	spectroscopy					708:719	confocal Raman spectroscopy	693:719	confocal Raman spectroscopy	693:719	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	11	2	theme	drug	1268:1271	arg1	release					1273:1279	drug release	1268:1279	drug release	1268:1279	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	4	3	theme	CTAB	579:582	arg1	solutions					593:601	CTAB micellar solutions	579:601	CTAB micellar solutions	579:601	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	4	4	theme	Drug	521:524	arg1	release					526:532	Drug release	521:532	Drug release	521:532	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	5	5	theme	Melatonin	658:666	arg1	release					668:674	Melatonin release	658:674	Melatonin release	658:674	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	11	6	theme	M	1363:1363	arg1	domains					1365:1371	M domains	1363:1371	M domains	1363:1371	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	1	7	theme	whole	169:173	arg1	range					175:179	the whole range	165:179	the whole range of compositions	165:195	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	2	8	from	implants	327:334	arg1	release					293:299	its release	289:299	its release from solid ethylcellulose implants	289:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	10	9	theme	Melatonin	1228:1236	arg1	transport					1215:1223	A micellar transport	1204:1223	CONCLUSION A micellar transport of Melatonin	1193:1236	CONCLUSION A micellar transport of Melatonin was found.					
29110612	2	10	theme	kinetic	349:355	arg1	point					372:376	both a kinetic and structural point	342:376	point	372:376	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	11	11	from	regimes	1257:1263	arg1	release					1273:1279	drug release	1268:1279	drug release	1268:1279	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	11	12	theme	polymer	1314:1320	arg1	effect					1330:1335	the polymer barrier effect	1310:1335	the polymer barrier effect	1310:1335	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	5	13	theme	2D	608:609	arg1	imaging					611:617	The 2D imaging	604:617	The 2D imaging of implant chemical composition during Melatonin release	604:674	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	6	14	theme	FT-IR	722:726	arg1	spectroscopy					728:739	FT-IR spectroscopy	722:739	FT-IR spectroscopy	722:739	FT-IR spectroscopy and Karl-Fisher technique were employed to study implants hydration.					
29110612	8	15	theme	apparent	890:897	arg1	D					922:922	D	922:922	D	922:922	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	8	15	theme	apparent	890:897	arg1	coefficient					909:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	4	16	theme	micellar	584:591	arg1	solutions					593:601	CTAB micellar solutions	579:601	CTAB micellar solutions	579:601	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	2	17	dep	point	372:376	arg1	both					342:345	both	342:345	both	342:345	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	2	18	theme	view	381:384	arg1	point					372:376	both a kinetic and structural point	342:376	point	372:376	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	11	19	dep	effect	1330:1335	arg1	connectivity					1373:1384	connectivity	1373:1384	connectivity	1373:1384	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	3	20	dep	implants	406:413	arg1	fabricated					470:479	fabricated	470:479	were fabricated to cover a large range of compositions	465:518	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	2	21	theme	loading	278:284	arg1	influence					251:259	the influence	247:259	the influence of the melatonin loading on its release from solid ethylcellulose implants	247:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	8	22	dep	10-12	1018:1022	arg1	to					1013:1014	to	1013:1014	to	1013:1014	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	3	23	theme	Ethylcellulose	444:457	arg1	ratio					459:463	Ethylcellulose ratio	444:463	Ethylcellulose ratio	444:463	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	4	24	from	tests	570:574	arg1	solutions					593:601	CTAB micellar solutions	579:601	CTAB micellar solutions	579:601	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	4	25	dep	in	549:550	arg1	vitro					552:556	vitro	552:556	vitro	552:556	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	5	26	theme	chemical	630:637	arg1	composition					639:649	implant chemical composition	622:649	implant chemical composition	622:649	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	5	27	theme	implant	622:628	arg1	composition					639:649	implant chemical composition	622:649	implant chemical composition	622:649	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	1	28	theme	Melatonin	94:102	arg1	release					104:110	BACKGROUND Melatonin release	83:110	BACKGROUND Melatonin release from Ethylcellulose matrix	83:137	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	8	29	theme	diffusion	899:907	arg1	D					922:922	D	922:922	D	922:922	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	8	29	theme	diffusion	899:907	arg1	coefficient					909:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	1	30	theme	compositions	184:195	arg1	range					175:179	the whole range	165:179	the whole range of compositions	165:195	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	3	31	theme	METHOD	387:392	arg1	implants					406:413	METHOD Cylindrical implants	387:413	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.	387:519	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	5	32	theme	composition	639:649	arg1	imaging					611:617	The 2D imaging	604:617	The 2D imaging of implant chemical composition during Melatonin release	604:674	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	1	33	from	matrix	132:137	arg1	release					104:110	BACKGROUND Melatonin release	83:110	BACKGROUND Melatonin release from Ethylcellulose matrix	83:137	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	2	34	theme	ethylcellulose	312:325	arg1	implants					327:334	solid ethylcellulose implants	306:334	solid ethylcellulose implants	306:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	7	35	theme	implant	854:860	arg1	composition					862:872	the implant composition	850:872	the implant composition	850:872	RESULTS A drug radial leakage, whatever the implant composition, is imaged.					
29110612	4	36	theme	in	549:550	arg1	tests					570:574	in vitro dissolution tests	549:574	in vitro dissolution tests in CTAB micellar solutions	549:601	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	2	37	theme	solid	306:310	arg1	implants					327:334	solid ethylcellulose implants	306:334	solid ethylcellulose implants	306:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	2	38	from	influence	251:259	arg1	release					293:299	its release	289:299	its release from solid ethylcellulose implants	289:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	0	39	theme	Implants	13:20	arg1	Influence					0:8	Influence	0:8	Influence of Implants	0:20	Influence of Implants Composition on Melatonin Release from Ethylcellulose Matrix.					
29110612	7	40	theme	drug	820:823	arg1	leakage					832:838	A drug radial leakage	818:838	A drug radial leakage	818:838	RESULTS A drug radial leakage, whatever the implant composition, is imaged.					
29110612	8	41	theme	Fickian	963:969	arg1	diffusion					978:986	Fickian radial diffusion	963:986	Fickian radial diffusion	963:986	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	1	42	theme	Ethylcellulose	117:130	arg1	matrix					132:137	Ethylcellulose matrix	117:137	Ethylcellulose matrix	117:137	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	8	43	theme	melatonin	927:935	arg1	D					922:922	D	922:922	D	922:922	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	8	43	theme	melatonin	927:935	arg1	coefficient					909:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient	886:919	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	9	44	theme	delivery	1100:1107	arg1	time					1109:1112	the characteristic drug delivery time	1076:1112	the characteristic drug delivery time	1076:1112	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	0	45	theme	Melatonin	37:45	arg1	Release					47:53	Melatonin Release	37:53	Melatonin Release from Ethylcellulose Matrix	37:80	Influence of Implants Composition on Melatonin Release from Ethylcellulose Matrix.					
29110612	4	46	theme	dissolution	558:568	arg1	tests					570:574	in vitro dissolution tests	549:574	in vitro dissolution tests in CTAB micellar solutions	549:601	Drug release was assayed by in vitro dissolution tests in CTAB micellar solutions.					
29110612	11	47	theme	initial	1342:1348	arg1	porosity					1350:1357	the initial porosity	1338:1357	the initial porosity	1338:1357	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	9	48	theme	drug	1095:1098	arg1	time					1109:1112	the characteristic drug delivery time	1076:1112	the characteristic drug delivery time	1076:1112	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	0	49	from	Matrix	75:80	arg1	Release					47:53	Melatonin Release	37:53	Melatonin Release from Ethylcellulose Matrix	37:80	Influence of Implants Composition on Melatonin Release from Ethylcellulose Matrix.					
29110612	6	50	theme	implants	790:797	arg1	hydration					799:807	implants hydration	790:807	implants hydration	790:807	FT-IR spectroscopy and Karl-Fisher technique were employed to study implants hydration.					
29110612	3	51	theme	Cylindrical	394:404	arg1	implants					406:413	METHOD Cylindrical implants	387:413	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.	387:519	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	7	52	theme	radial	825:830	arg1	leakage					832:838	A drug radial leakage	818:838	A drug radial leakage	818:838	RESULTS A drug radial leakage, whatever the implant composition, is imaged.					
29110612	3	53	theme	large	492:496	arg1	range					498:502	a large range	490:502	a large range of compositions	490:518	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	10	54	dep	CONCLUSION	1193:1202	arg1	transport					1215:1223	A micellar transport	1204:1223	CONCLUSION A micellar transport of Melatonin	1193:1236	CONCLUSION A micellar transport of Melatonin was found.					
29110612	2	55	theme	comprehensive	221:233	arg1	study					235:239	a comprehensive study	219:239	a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants	219:334	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	3	56	theme	compositions	507:518	arg1	range					498:502	a large range	490:502	a large range of compositions	490:518	METHOD Cylindrical implants differing in their Melatonin:Ethylcellulose ratio were fabricated to cover a large range of compositions.					
29110612	8	57	theme	EC	1047:1048	arg1	content					1050:1056	the EC content	1043:1056	the EC content	1043:1056	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	5	58	theme	confocal	693:700	arg1	spectroscopy					708:719	confocal Raman spectroscopy	693:719	confocal Raman spectroscopy	693:719	The 2D imaging of implant chemical composition during Melatonin release was performed by confocal Raman spectroscopy.					
29110612	2	59	theme	melatonin	268:276	arg1	loading					278:284	the melatonin loading	264:284	the melatonin loading	264:284	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
29110612	10	60	theme	micellar	1206:1213	arg1	transport					1215:1223	A micellar transport	1204:1223	CONCLUSION A micellar transport of Melatonin	1193:1236	CONCLUSION A micellar transport of Melatonin was found.					
29110612	1	61	theme	BACKGROUND	83:92	arg1	release					104:110	BACKGROUND Melatonin release	83:110	BACKGROUND Melatonin release from Ethylcellulose matrix	83:137	BACKGROUND Melatonin release from Ethylcellulose matrix has never been studied on the whole range of compositions.					
29110612	9	62	theme	different	1158:1166	arg1	regimes					1168:1174	two different regimes	1154:1174	two different regimes	1154:1174	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	9	63	theme	time	1109:1112	arg1	non-monotonous					1135:1148	non-monotonous	1135:1148	non-monotonous	1135:1148	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	9	63	theme	time	1109:1112	arg1	variation					1063:1071	The variation	1059:1071	The variation of the characteristic drug delivery time with composition	1059:1129	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	9	64	theme	characteristic	1080:1093	arg1	time					1109:1112	the characteristic drug delivery time	1076:1112	the characteristic drug delivery time	1076:1112	The variation of the characteristic drug delivery time with composition was non-monotonous and two different regimes were identified.					
29110612	8	65	theme	radial	971:976	arg1	diffusion					978:986	Fickian radial diffusion	963:986	Fickian radial diffusion	963:986	The apparent diffusion coefficient, D of melatonin was evaluated considering Fickian radial diffusion: its value ranges from 2 to 6 10-12 cm2/s depending on the EC content.					
29110612	0	66	theme	Ethylcellulose	60:73	arg1	Matrix					75:80	Ethylcellulose Matrix	60:80	Ethylcellulose Matrix	60:80	Influence of Implants Composition on Melatonin Release from Ethylcellulose Matrix.					
29110612	0	67	from	Composition	22:32	arg1	Release					47:53	Melatonin Release	37:53	Melatonin Release from Ethylcellulose Matrix	37:80	Influence of Implants Composition on Melatonin Release from Ethylcellulose Matrix.					
29110612	11	68	theme	barrier	1322:1328	arg1	effect					1330:1335	the polymer barrier effect	1310:1335	the polymer barrier effect	1310:1335	The two regimes in drug release were interpreted considering the polymer barrier effect, the initial porosity and M domains connectivity.					
29110612	6	69	theme	Karl-Fisher	745:755	arg1	technique					757:765	Karl-Fisher technique	745:765	Karl-Fisher technique	745:765	FT-IR spectroscopy and Karl-Fisher technique were employed to study implants hydration.					
29110612	2	70	theme	structural	361:370	arg1	point					372:376	both a kinetic and structural point	342:376	point	372:376	OBJECTIVE To perform a comprehensive study about the influence of the melatonin loading on its release from solid ethylcellulose implants, from both a kinetic and structural point of view.					
31197074	9	0	theme	acid	882:885	arg1	content					858:864	their high content	847:864	their high content of galacturonic acid and the extraction method	847:911	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	10	1	theme	scanning	1035:1042	arg1	DSC					1057:1059	DSC	1057:1059	DSC	1057:1059	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	10	1	theme	scanning	1035:1042	arg1	calorimetry					1044:1054	differential scanning calorimetry	1022:1054	differential scanning calorimetry (DSC)	1022:1060	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	4	2	contain	has	196:198	arg2	functions					227:235	nutritional and healthcare functions	200:235	nutritional and healthcare functions	200:235	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	4	2	contain	has	196:198	arg1	It					193:194	It	193:194	It	193:194	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	7	3	theme	response	601:608	arg1	method					618:623	the response surface method	597:623	the response surface method	597:623	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	8	4	dep	composition	636:646	arg1	The					626:628	The	626:628	The	626:628	The basic composition and antioxidant effects of ZJRP were determined.					
31197074	0	5	theme	jujuba	82:87	arg1	cv					89:90	Ziziphus jujuba cv	73:90	Ziziphus jujuba cv	73:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	10	6	theme	differential	1022:1033	arg1	DSC					1057:1059	DSC	1057:1059	DSC	1057:1059	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	10	6	theme	differential	1022:1033	arg1	calorimetry					1044:1054	differential scanning calorimetry	1022:1054	differential scanning calorimetry (DSC)	1022:1060	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	9	7	theme	DPPH	793:796	arg1	related					836:842	related	836:842	related	836:842	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	7	theme	DPPH	793:796	arg1	rate					817:820	the high DPPH radical scavenging rate	784:820	the high DPPH radical scavenging rate	784:820	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	4	8	theme	nutritional	200:210	arg1	functions					227:235	nutritional and healthcare functions	200:235	nutritional and healthcare functions	200:235	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	0	9	from	Optimization	0:11	arg1	cv					89:90	Ziziphus jujuba cv	73:90	Ziziphus jujuba cv	73:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	6	10	theme	food	414:417	arg1	ingredients					419:429	new functional food ingredients	399:429	new functional food ingredients	399:429	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	6	10	theme	food	414:417	arg1	polysaccharide					436:449	the polysaccharide	432:449	the polysaccharide (ZJRP)	432:456	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	3	11	theme	Yellow	164:169	arg1	Basin					177:181	the Yellow River Basin	160:181	the Yellow River Basin of China	160:190	Muzao is a plant widely cultivated in the Yellow River Basin of China.					
31197074	5	12	theme	Ziziphus	343:350	arg1	cv					359:360	Ziziphus jujuba cv	343:360	Ziziphus jujuba cv	343:360	In order to make effective use of Ziziphus jujuba cv.					
31197074	9	13	theme	extraction	895:904	arg1	method					906:911	the extraction method	891:911	the extraction method	891:911	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	8	14	theme	antioxidant	652:662	arg1	effects					664:670	antioxidant effects	652:670	antioxidant effects	652:670	The basic composition and antioxidant effects of ZJRP were determined.					
31197074	6	15	theme	functional	403:412	arg1	ingredients					419:429	new functional food ingredients	399:429	new functional food ingredients	399:429	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	6	15	theme	functional	403:412	arg1	polysaccharide					436:449	the polysaccharide	432:449	the polysaccharide (ZJRP)	432:456	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	11	16	theme	functional	1321:1330	arg1	ZJRP					1287:1290	ZJRP	1287:1290	ZJRP	1287:1290	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	16	theme	functional	1321:1330	arg1	ingredient					1337:1346	a functional food ingredient	1319:1346	a functional food ingredient	1319:1346	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	4	17	theme	main	271:274	arg1	polysaccharides					247:261	polysaccharides	247:261	polysaccharides	247:261	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	4	17	theme	main	271:274	arg1	components					276:285	the main components	267:285	the main components of its bio-functions	267:306	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	5	18	theme	jujuba	352:357	arg1	cv					359:360	Ziziphus jujuba cv	343:360	Ziziphus jujuba cv	343:360	In order to make effective use of Ziziphus jujuba cv.					
31197074	9	19	theme	method	906:911	arg1	content					858:864	their high content	847:864	their high content of galacturonic acid and the extraction method	847:911	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	6	20	from	cv	479:480	arg1	ingredients					419:429	new functional food ingredients	399:429	new functional food ingredients	399:429	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	6	20	from	cv	479:480	arg1	polysaccharide					436:449	the polysaccharide	432:449	the polysaccharide (ZJRP)	432:456	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	6	21	theme	new	399:401	arg1	ingredients					419:429	new functional food ingredients	399:429	new functional food ingredients	399:429	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	6	21	theme	new	399:401	arg1	polysaccharide					436:449	the polysaccharide	432:449	the polysaccharide (ZJRP)	432:456	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	7	22	theme	optimal	542:548	arg1	conditions					561:570	the optimal extraction conditions	538:570	the optimal extraction conditions of ZJRP	538:578	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	0	23	from	Properties	38:47	arg1	cv					89:90	Ziziphus jujuba cv	73:90	Ziziphus jujuba cv	73:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	9	24	theme	scavenging	806:815	arg1	related					836:842	related	836:842	related	836:842	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	24	theme	scavenging	806:815	arg1	rate					817:820	the high DPPH radical scavenging rate	784:820	the high DPPH radical scavenging rate	784:820	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	7	25	theme	extraction	550:559	arg1	conditions					561:570	the optimal extraction conditions	538:570	the optimal extraction conditions of ZJRP	538:578	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	4	26	from	components	276:285	arg1	functions					227:235	nutritional and healthcare functions	200:235	nutritional and healthcare functions	200:235	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	11	27	theme	potential	1211:1219	arg1	method					1187:1192	the alkaline extraction method	1163:1192	the alkaline extraction method	1163:1192	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	27	theme	potential	1211:1219	arg1	technique					1221:1229	a potential technique	1209:1229	a potential technique for extracting ZJRP with high antioxidant activity	1209:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	3	28	theme	China	186:190	arg1	Basin					177:181	the Yellow River Basin	160:181	the Yellow River Basin of China	160:190	Muzao is a plant widely cultivated in the Yellow River Basin of China.					
31197074	3	29	theme	River	171:175	arg1	Basin					177:181	the Yellow River Basin	160:181	the Yellow River Basin of China	160:190	Muzao is a plant widely cultivated in the Yellow River Basin of China.					
31197074	9	30	theme	radical	798:804	arg1	related					836:842	related	836:842	related	836:842	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	30	theme	radical	798:804	arg1	rate					817:820	the high DPPH radical scavenging rate	784:820	the high DPPH radical scavenging rate	784:820	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	0	31	theme	Extraction	23:32	arg1	Properties					38:47	Properties	38:47	Properties of Polysaccharides from Ziziphus jujuba cv	38:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	0	31	theme	Extraction	23:32	arg1	Optimization					0:11	Optimization	0:11	Optimization of Alkali Extraction	0:32	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	8	32	theme	ZJRP	675:678	arg1	composition					636:646	basic composition	630:646	basic composition	630:646	The basic composition and antioxidant effects of ZJRP were determined.					
31197074	8	32	theme	ZJRP	675:678	arg1	effects					664:670	antioxidant effects	652:670	antioxidant effects	652:670	The basic composition and antioxidant effects of ZJRP were determined.					
31197074	6	33	theme	jujuba	472:477	arg1	cv					479:480	Ziziphus jujuba cv	463:480	Ziziphus jujuba cv	463:480	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	0	34	theme	Alkali	16:21	arg1	Extraction					23:32	Alkali Extraction	16:32	Alkali Extraction	16:32	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	6	35	theme	Ziziphus	463:470	arg1	cv					479:480	Ziziphus jujuba cv	463:480	Ziziphus jujuba cv	463:480	Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv.					
31197074	0	36	from	cv	89:90	arg1	Properties					38:47	Properties	38:47	Properties of Polysaccharides from Ziziphus jujuba cv	38:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	0	36	from	cv	89:90	arg1	Optimization					0:11	Optimization	0:11	Optimization of Alkali Extraction	0:32	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	0	36	from	cv	89:90	arg1	Polysaccharides					52:66	Polysaccharides	52:66	Polysaccharides from Ziziphus jujuba cv	52:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	5	37	theme	effective	326:334	arg1	use					336:338	effective use	326:338	effective use of Ziziphus jujuba cv	326:360	In order to make effective use of Ziziphus jujuba cv.					
31197074	11	38	theme	alkaline	1167:1174	arg1	method					1187:1192	the alkaline extraction method	1163:1192	the alkaline extraction method	1163:1192	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	38	theme	alkaline	1167:1174	arg1	technique					1221:1229	a potential technique	1209:1229	a potential technique for extracting ZJRP with high antioxidant activity	1209:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	2	39	theme	Ziziphus	102:109	arg1	cv					118:119	Ziziphus jujuba cv	102:119	Ziziphus jujuba cv.	102:120	Ziziphus jujuba cv.					
31197074	11	40	theme	extraction	1176:1185	arg1	method					1187:1192	the alkaline extraction method	1163:1192	the alkaline extraction method	1163:1192	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	40	theme	extraction	1176:1185	arg1	technique					1221:1229	a potential technique	1209:1229	a potential technique for extracting ZJRP with high antioxidant activity	1209:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	10	41	theme	ZJRP	969:972	arg1	properties					955:964	the rheological and thermal properties	927:964	the rheological and thermal properties of ZJRP	927:972	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	7	42	theme	sodium	516:521	arg1	hydroxide					523:531	sodium hydroxide	516:531	sodium hydroxide	516:531	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	10	43	theme	shear	1089:1093	arg1	properties					1104:1113	shear thinning properties	1089:1113	shear thinning properties	1089:1113	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	0	44	theme	Polysaccharides	52:66	arg1	Properties					38:47	Properties	38:47	Properties of Polysaccharides from Ziziphus jujuba cv	38:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	0	44	theme	Polysaccharides	52:66	arg1	Optimization					0:11	Optimization	0:11	Optimization of Alkali Extraction	0:32	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	11	45	theme	food	1332:1335	arg1	ZJRP					1287:1290	ZJRP	1287:1290	ZJRP	1287:1290	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	45	theme	food	1332:1335	arg1	ingredient					1337:1346	a functional food ingredient	1319:1346	a functional food ingredient	1319:1346	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	9	46	theme	significant	730:740	arg1	activity					754:761	significant antioxidant activity	730:761	significant antioxidant activity	730:761	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	7	47	theme	ZJRP	575:578	arg1	conditions					561:570	the optimal extraction conditions	538:570	the optimal extraction conditions of ZJRP	538:578	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	11	48	theme	antioxidant	1261:1271	arg1	activity					1273:1280	high antioxidant activity	1256:1280	high antioxidant activity	1256:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	2	49	theme	jujuba	111:116	arg1	cv					118:119	Ziziphus jujuba cv	102:119	Ziziphus jujuba cv.	102:120	Ziziphus jujuba cv.					
31197074	10	50	theme	thinning	1095:1102	arg1	properties					1104:1113	shear thinning properties	1089:1113	shear thinning properties	1089:1113	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	11	51	theme	high	1256:1259	arg1	activity					1273:1280	high antioxidant activity	1256:1280	high antioxidant activity	1256:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	9	52	theme	antioxidant	742:752	arg1	activity					754:761	significant antioxidant activity	730:761	significant antioxidant activity	730:761	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	53	theme	high	788:791	arg1	related					836:842	related	836:842	related	836:842	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	53	theme	high	788:791	arg1	rate					817:820	the high DPPH radical scavenging rate	784:820	the high DPPH radical scavenging rate	784:820	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	4	54	theme	healthcare	216:225	arg1	functions					227:235	nutritional and healthcare functions	200:235	nutritional and healthcare functions	200:235	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	9	55	contain	has	726:728	arg1	ZJRP					721:724	ZJRP	721:724	ZJRP	721:724	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	55	contain	has	726:728	arg2	activity					754:761	significant antioxidant activity	730:761	significant antioxidant activity	730:761	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	9	56	theme	high	853:856	arg1	content					858:864	their high content	847:864	their high content of galacturonic acid and the extraction method	847:911	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	7	57	theme	Muzao	483:487	arg1	residues					489:496	Muzao residues	483:496	Muzao residues	483:496	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	10	58	contain	have	1084:1087	arg1	they					1079:1082	they	1079:1082	they	1079:1082	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	10	58	contain	have	1084:1087	arg2	properties					1104:1113	shear thinning properties	1089:1113	shear thinning properties	1089:1113	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	10	58	contain	have	1084:1087	arg2	stability					1132:1140	good thermal stability	1119:1140	good thermal stability	1119:1140	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	11	59	used	used	1201:1204	arg2	technique					1221:1229	a potential technique	1209:1229	a potential technique for extracting ZJRP with high antioxidant activity	1209:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	11	59	used	used	1201:1204	arg2	method					1187:1192	the alkaline extraction method	1163:1192	the alkaline extraction method	1163:1192	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	5	60	theme	cv	359:360	arg1	use					336:338	effective use	326:338	effective use of Ziziphus jujuba cv	326:360	In order to make effective use of Ziziphus jujuba cv.					
31197074	10	61	theme	thermal	947:953	arg1	properties					955:964	the rheological and thermal properties	927:964	the rheological and thermal properties of ZJRP	927:972	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	4	62	theme	bio-functions	294:306	arg1	polysaccharides					247:261	polysaccharides	247:261	polysaccharides	247:261	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	4	62	theme	bio-functions	294:306	arg1	components					276:285	the main components	267:285	the main components of its bio-functions	267:306	It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions.					
31197074	8	63	theme	basic	630:634	arg1	composition					636:646	basic composition	630:646	basic composition	630:646	The basic composition and antioxidant effects of ZJRP were determined.					
31197074	0	64	theme	Ziziphus	73:80	arg1	cv					89:90	Ziziphus jujuba cv	73:90	Ziziphus jujuba cv	73:90	Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv.					
31197074	11	65	with	ZJRP	1246:1249	arg1	activity					1273:1280	high antioxidant activity	1256:1280	high antioxidant activity	1256:1280	Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.					
31197074	10	66	theme	thermal	1124:1130	arg1	stability					1132:1140	good thermal stability	1119:1140	good thermal stability	1119:1140	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	9	67	theme	galacturonic	869:880	arg1	acid					882:885	galacturonic acid	869:885	galacturonic acid	869:885	The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method.					
31197074	7	68	theme	surface	610:616	arg1	method					618:623	the response surface method	597:623	the response surface method	597:623	Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method.					
31197074	10	69	theme	rheological	931:941	arg1	properties					955:964	the rheological and thermal properties	927:964	the rheological and thermal properties of ZJRP	927:972	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31197074	10	70	theme	good	1119:1122	arg1	stability					1132:1140	good thermal stability	1119:1140	good thermal stability	1119:1140	In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability.					
31389736	0	0	theme	rheological	56:66	arg1	study					68:72	rheological study	56:72	rheological study	56:72	Endotracheal tube mucus as a source of airway mucus for rheological study.					
31389736	2	1	theme	mucus	330:334	arg1	clearance					336:344	mucus clearance	330:344	mucus clearance	330:344	In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance.					
31389736	4	2	theme	in	550:551	arg1	production					558:567	in vivo production	550:567	in vivo production	550:567	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	6	3	theme	spatial	887:893	arg1	scales					895:900	spatial scales	887:900	spatial scales	887:900	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	6	4	theme	concentration-dependent	823:845	arg1	properties					857:866	concentration-dependent rheologic properties	823:866	concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus	823:951	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	4	5	theme	bronchial	622:630	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	3	6	theme	MOLD	347:350	arg1	research					352:359	MOLD research	347:359	MOLD research	347:359	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	4	7	theme	endotracheal	476:487	arg1	tubes					489:493	endotracheal tubes	476:493	endotracheal tubes (ETT)	476:499	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	7	theme	endotracheal	476:487	arg1	ETT					496:498	ETT	496:498	ETT	496:498	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	5	8	theme	mucus	698:702	arg1	compositions					678:689	Ionic and biochemical compositions	656:689	Ionic and biochemical compositions of ETT mucus from healthy human subjects	656:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	1	9	theme	chronic	140:146	arg1	disease					170:176	chronic obstructive pulmonary disease	140:176	chronic obstructive pulmonary disease	140:176	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	2	10	theme	airway	233:238	arg1	mucus					240:244	the airway mucus	229:244	the airway mucus	229:244	In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance.					
31389736	1	11	theme	obstructive	148:158	arg1	disease					170:176	chronic obstructive pulmonary disease	140:176	chronic obstructive pulmonary disease	140:176	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	3	12	theme	relevant	370:377	arg1	sources					379:385	relevant sources	370:385	relevant sources of healthy airway mucus for experimental manipulation and analysis	370:452	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	7	13	theme	MOLD	1064:1067	arg1	research					1069:1076	MOLD research	1064:1076	MOLD research	1064:1076	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	1	14	theme	pulmonary	160:168	arg1	disease					170:176	chronic obstructive pulmonary disease	140:176	chronic obstructive pulmonary disease	140:176	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	0	15	theme	Endotracheal	0:11	arg1	tube					13:16	Endotracheal tube	0:16	Endotracheal tube	0:16	Endotracheal tube mucus as a source of airway mucus for rheological study.					
31389736	1	16	theme	Muco-obstructive	75:90	arg1	MOLDs					107:111	MOLDs	107:111	MOLDs	107:111	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	1	16	theme	Muco-obstructive	75:90	arg1	diseases					97:104	Muco-obstructive lung diseases	75:104	Muco-obstructive lung diseases (MOLDs)	75:112	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	3	17	theme	experimental	415:426	arg1	manipulation					428:439	experimental manipulation	415:439	experimental manipulation	415:439	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	5	18	theme	ETT	773:775	arg1	samples					777:783	pooled ETT samples	766:783	pooled ETT samples	766:783	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	5	19	theme	pooled	766:771	arg1	samples					777:783	pooled ETT samples	766:783	pooled ETT samples	766:783	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	5	20	theme	Ionic	656:660	arg1	compositions					678:689	Ionic and biochemical compositions	656:689	Ionic and biochemical compositions of ETT mucus from healthy human subjects	656:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	1	21	theme	lung	92:95	arg1	MOLDs					107:111	MOLDs	107:111	MOLDs	107:111	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	1	21	theme	lung	92:95	arg1	diseases					97:104	Muco-obstructive lung diseases	75:104	Muco-obstructive lung diseases (MOLDs)	75:112	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	2	22	theme	osmotic	284:290	arg1	moduli					309:314	osmotic and viscoelastic moduli	284:314	osmotic and viscoelastic moduli	284:314	In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance.					
31389736	6	23	theme	ETT	803:805	arg1	mucus					807:811	Pooled ETT mucus	796:811	Pooled ETT mucus	796:811	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	6	24	theme	rheologic	847:855	arg1	properties					857:866	concentration-dependent rheologic properties	823:866	concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus	823:951	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	3	25	theme	airway	398:403	arg1	mucus					405:409	healthy airway mucus	390:409	healthy airway mucus	390:409	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	6	26	theme	HBE	943:945	arg1	mucus					947:951	HBE mucus	943:951	HBE mucus	943:951	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	5	27	theme	biochemical	666:676	arg1	compositions					678:689	Ionic and biochemical compositions	656:689	Ionic and biochemical compositions of ETT mucus from healthy human subjects	656:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	4	28	dep	production	558:567	arg1	e.g.					544:547	e.g.	544:547	e.g.	544:547	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	29	theme	epithelial	632:641	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	3	30	theme	mucus	405:409	arg1	sources					379:385	relevant sources	370:385	relevant sources of healthy airway mucus for experimental manipulation and analysis	370:452	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	5	31	theme	ETT	694:696	arg1	mucus					698:702	ETT mucus	694:702	ETT mucus	694:702	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	0	32	theme	airway	39:44	arg1	mucus					46:50	airway mucus	39:50	airway mucus	39:50	Endotracheal tube mucus as a source of airway mucus for rheological study.					
31389736	7	33	theme	useful	1053:1058	arg1	mucus					1035:1039	ETT mucus	1031:1039	ETT mucus potentially useful for MOLD research	1031:1076	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	4	34	dep	in	550:551	arg1	vivo					553:556	vivo	553:556	vivo	553:556	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	7	35	theme	other	1007:1011	arg1	types					1020:1024	other sample types	1007:1024	other sample types	1007:1024	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	3	36	theme	healthy	390:396	arg1	mucus					405:409	healthy airway mucus	390:409	healthy airway mucus	390:409	MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis.					
31389736	6	37	theme	ETT	927:929	arg1	samples					931:937	reported individual ETT samples	907:937	reported individual ETT samples	907:937	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	7	38	theme	ETT	1031:1033	arg1	mucus					1035:1039	ETT mucus	1031:1039	ETT mucus potentially useful for MOLD research	1031:1076	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	5	39	from	subjects	723:730	arg1	compositions					678:689	Ionic and biochemical compositions	656:689	Ionic and biochemical compositions of ETT mucus from healthy human subjects	656:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	6	40	theme	individual	916:925	arg1	samples					931:937	reported individual ETT samples	907:937	reported individual ETT samples	907:937	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	7	41	theme	sample	1013:1018	arg1	types					1020:1024	other sample types	1007:1024	other sample types	1007:1024	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	6	42	theme	Pooled	796:801	arg1	mucus					807:811	Pooled ETT mucus	796:811	Pooled ETT mucus	796:811	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	4	43	theme	HBE	644:646	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	44	theme	canonical	575:583	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	44	theme	canonical	575:583	arg1	sputum					606:611	sputum	606:611	sputum	606:611	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	44	theme	canonical	575:583	arg1	types					592:596	canonical sample types	575:596	canonical sample types such as sputum or human bronchial epithelial (HBE) mucus	575:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	5	45	theme	human	717:721	arg1	subjects					723:730	healthy human subjects	709:730	healthy human subjects	709:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	2	46	theme	viscoelastic	296:307	arg1	moduli					309:314	osmotic and viscoelastic moduli	284:314	osmotic and viscoelastic moduli	284:314	In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance.					
31389736	1	47	theme	subjects	200:207	arg1	spectrum					188:195	a spectrum	186:195	a spectrum of subjects	186:207	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	5	48	theme	healthy	709:715	arg1	subjects					723:730	healthy human subjects	709:730	healthy human subjects	709:730	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	7	49	theme	practical	974:982	arg1	benefits					984:991	the practical benefits	970:991	the practical benefits compared with other sample types	970:1024	We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.					
31389736	4	50	theme	human	616:620	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	0	51	theme	mucus	46:50	arg1	source					29:34	a source	27:34	a source of airway mucus for rheological study	27:72	Endotracheal tube mucus as a source of airway mucus for rheological study.					
31389736	6	52	theme	reported	907:914	arg1	samples					931:937	reported individual ETT samples	907:937	reported individual ETT samples	907:937	Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus.					
31389736	5	53	theme	samples	777:783	arg1	stock					757:761	a stock	755:761	a stock of pooled ETT samples	755:783	Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated.					
31389736	1	54	theme	cystic	120:125	arg1	fibrosis					127:134	cystic fibrosis	120:134	cystic fibrosis	120:134	Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally.					
31389736	4	55	theme	sample	585:590	arg1	mucus					649:653	human bronchial epithelial (HBE) mucus	616:653	human bronchial epithelial (HBE) mucus	616:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	55	theme	sample	585:590	arg1	sputum					606:611	sputum	606:611	sputum	606:611	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31389736	4	55	theme	sample	585:590	arg1	types					592:596	canonical sample types	575:596	canonical sample types such as sputum or human bronchial epithelial (HBE) mucus	575:653	Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus.					
31295903	9	0	theme	Quantitative	1394:1405	arg1	recoveries					1407:1416	Quantitative recoveries	1394:1416	Quantitative recoveries of these monosaccharides in the samples	1394:1456	Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10-103.70%.					
31295903	5	1	theme	trifluoroacetic	762:776	arg1	acid					778:781	4 mol/L trifluoroacetic acid	754:781	4 mol/L trifluoroacetic acid (TFA)	754:787	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	1	theme	trifluoroacetic	762:776	arg1	h					792:792	6 h	790:792	6 h	790:792	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	1	theme	trifluoroacetic	762:776	arg1	TFA					784:786	TFA	784:786	TFA	784:786	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	7	2	theme	spectral	1065:1072	arg1	behaviors					1074:1082	mass spectral behaviors	1060:1082	mass spectral behaviors	1060:1082	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	4	3	theme	hydrolysis	587:596	arg1	conditions					598:607	The hydrolysis conditions	583:607	The hydrolysis conditions of the polysaccharides	583:630	The hydrolysis conditions of the polysaccharides were optimized by orthogonal design.					
31295903	10	4	theme	quality	1629:1635	arg1	control					1637:1643	the quality control	1625:1643	the quality control of Polyporusumbellatus	1625:1666	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	0	5	from	Analysis	13:20	arg1	umbellatus					65:74	Polyporus umbellatus	55:74	Polyporus umbellatus	55:74	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	5	6	dep	°C	799:800	arg1	TFA					828:830	3 mol/L TFA	820:830	3 mol/L TFA	820:830	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	10	7	theme	Polyporusumbellatus	1648:1666	arg1	control					1637:1643	the quality control	1625:1643	the quality control of Polyporusumbellatus	1625:1666	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	5	8	theme	neutral	722:728	arg1	sugars					730:735	neutral sugars	722:735	neutral sugars	722:735	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	6	9	theme	established	969:979	arg1	method					981:986	the established method	965:986	the established method	965:986	The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method.					
31295903	5	10	dep	were	705:708	arg1	sugars					730:735	neutral sugars	722:735	neutral sugars	722:735	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	10	dep	were	705:708	arg1	acids					748:752	uronic acids	741:752	uronic acids	741:752	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	11	theme	amino	807:811	arg1	sugars					813:818	amino sugars	807:818	amino sugars	807:818	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	11	theme	amino	807:811	arg1	h					792:792	6 h	790:792	6 h	790:792	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	0	12	from	umbellatus	65:74	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.	0:94	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	3	13	theme	monosaccharides	388:402	arg1	analysis					373:380	the analysis	369:380	the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry	369:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	9	14	theme	monosaccharides	1427:1441	arg1	recoveries					1407:1416	Quantitative recoveries	1394:1416	Quantitative recoveries of these monosaccharides in the samples	1394:1456	Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10-103.70%.					
31295903	5	15	theme	uronic	741:746	arg1	acids					748:752	uronic acids	741:752	uronic acids	741:752	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	8	16	theme	glucuronic	1318:1327	arg1	acid					1329:1332	glucuronic acid	1318:1332	glucuronic acid	1318:1332	The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose.					
31295903	3	17	theme	ionization-ion	529:542	arg1	trap-time					544:552	electrospray ionization-ion trap-time	516:552	electrospray ionization-ion trap-time of flight-mass spectrometry	516:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	5	18	theme	3	820:820	arg1	mol/L					822:826	mol/L	822:826	mol/L	822:826	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	9	19	from	recoveries	1407:1416	arg1	samples					1450:1456	the samples	1446:1456	the samples	1446:1456	Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10-103.70%.					
31295903	5	20	theme	hydrolysis	694:703	arg1	results					673:679	The results	669:679	The results of optimized hydrolysis	669:703	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	1	21	theme	Polyporus	96:104	arg1	fungus					157:162	a well-known and important medicinal fungus	120:162	a well-known and important medicinal fungus in Asia	120:170	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	1	21	theme	Polyporus	96:104	arg1	umbellatus					106:115	Polyporus umbellatus	96:115	Polyporus umbellatus	96:115	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	2	22	contain	possess	193:199	arg1	polysaccharides					177:191	Its polysaccharides	173:191	Its polysaccharides	173:191	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	2	22	contain	possess	193:199	arg2	bioactivities					213:225	interesting bioactivities	201:225	interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects	201:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	2	22	contain	possess	193:199	arg2	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	0	23	from	Composition	40:50	arg1	umbellatus					65:74	Polyporus umbellatus	55:74	Polyporus umbellatus	55:74	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	1	24	from	fungus	157:162	arg1	Asia					167:170	Asia	167:170	Asia	167:170	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	0	25	theme	Quantitative	0:11	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.	0:94	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	5	26	theme	mol/L	756:760	arg1	acid					778:781	4 mol/L trifluoroacetic acid	754:781	4 mol/L trifluoroacetic acid (TFA)	754:787	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	26	theme	mol/L	756:760	arg1	h					792:792	6 h	790:792	6 h	790:792	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	26	theme	mol/L	756:760	arg1	TFA					784:786	TFA	784:786	TFA	784:786	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	3	27	theme	electrospray	516:527	arg1	trap-time					544:552	electrospray ionization-ion trap-time	516:552	electrospray ionization-ion trap-time of flight-mass spectrometry	516:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	0	28	theme	Polysaccharide	25:38	arg1	Composition					40:50	Polysaccharide Composition	25:50	Polysaccharide Composition in Polyporus umbellatus	25:74	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	3	29	theme	quantitative	324:335	arg1	method					337:342	A qualitative and quantitative method	306:342	A qualitative and quantitative method	306:342	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	7	30	theme	monosaccharides	1011:1025	arg1	Identification					989:1002	Identification	989:1002	Identification of the monosaccharides	989:1025	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	6	31	theme	resulting	850:858	arg1	monosaccharides					860:874	The resulting monosaccharides	846:874	The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone	846:922	The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method.					
31295903	3	32	theme	spectrometry	569:580	arg1	trap-time					544:552	electrospray ionization-ion trap-time	516:552	electrospray ionization-ion trap-time of flight-mass spectrometry	516:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	3	33	theme	umbellatus	444:453	arg1	polysaccharides					415:429	polysaccharides	415:429	polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry	415:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	1	34	theme	well-known	122:131	arg1	fungus					157:162	a well-known and important medicinal fungus	120:162	a well-known and important medicinal fungus in Asia	120:170	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	1	34	theme	well-known	122:131	arg1	umbellatus					106:115	Polyporus umbellatus	96:115	Polyporus umbellatus	96:115	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	7	35	dep	behaviors	1074:1082	arg1	the					1056:1058	the	1056:1058	the	1056:1058	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	8	36	theme	galacturonic	1335:1346	arg1	acid					1348:1351	galacturonic acid	1335:1351	galacturonic acid	1335:1351	The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose.					
31295903	3	37	theme	qualitative	308:318	arg1	method					337:342	A qualitative and quantitative method	306:342	A qualitative and quantitative method	306:342	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	2	38	theme	interesting	201:211	arg1	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	2	38	theme	interesting	201:211	arg1	bioactivities					213:225	interesting bioactivities	201:225	interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects	201:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	0	39	theme	Composition	40:50	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.	0:94	Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS.					
31295903	4	40	theme	orthogonal	650:659	arg1	design					661:666	orthogonal design	650:666	orthogonal design	650:666	The hydrolysis conditions of the polysaccharides were optimized by orthogonal design.					
31295903	10	41	theme	monosaccharides	1586:1600	arg1	quantification					1568:1581	quantification	1568:1581	quantification	1568:1581	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	10	41	theme	monosaccharides	1586:1600	arg1	identification					1549:1562	identification	1549:1562	identification	1549:1562	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	2	42	theme	hepatoprotective	259:274	arg1	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	3	43	theme	flight-mass	557:567	arg1	spectrometry					569:580	flight-mass spectrometry	557:580	flight-mass spectrometry	557:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	3	44	theme	high-performance	464:479	arg1	chromatography					488:501	high-performance liquid chromatography	464:501	high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry	464:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	8	45	from	polysaccharides	1202:1216	arg1	umbellatus					1231:1240	Polyporus umbellatus	1221:1240	Polyporus umbellatus	1221:1240	The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose.					
31295903	7	46	theme	1-phenyl-3-methyl-5-pyrazolone	1122:1151	arg1	monosaccharides					1161:1175	1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides	1122:1175	1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides	1122:1175	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	3	47	theme	liquid	481:486	arg1	chromatography					488:501	high-performance liquid chromatography	464:501	high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry	464:580	A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization-ion trap-time of flight-mass spectrometry.					
31295903	10	48	dep	identification	1549:1562	arg1	the					1545:1547	the	1545:1547	the	1545:1547	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	2	49	theme	antioxidant	246:256	arg1	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	7	50	theme	chromatography	1088:1101	arg1	characteristics					1103:1117	chromatography characteristics	1088:1117	chromatography characteristics	1088:1117	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	1	51	theme	important	137:145	arg1	fungus					157:162	a well-known and important medicinal fungus	120:162	a well-known and important medicinal fungus in Asia	120:170	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	1	51	theme	important	137:145	arg1	umbellatus					106:115	Polyporus umbellatus	96:115	Polyporus umbellatus	96:115	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	5	52	dep	sugars	730:735	arg1	acid					778:781	4 mol/L trifluoroacetic acid	754:781	4 mol/L trifluoroacetic acid (TFA)	754:787	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	52	dep	sugars	730:735	arg1	h					792:792	6 h	790:792	6 h	790:792	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	5	52	dep	sugars	730:735	arg1	TFA					784:786	TFA	784:786	TFA	784:786	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	9	53	theme	%	1491:1491	arg1	range					1470:1474	the range	1466:1474	the range of 96.10-103.70%	1466:1491	Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10-103.70%.					
31295903	7	54	theme	labeled	1153:1159	arg1	monosaccharides					1161:1175	1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides	1122:1175	1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides	1122:1175	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	2	55	theme	antitumor	235:243	arg1	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	5	56	theme	optimized	684:692	arg1	hydrolysis					694:703	optimized hydrolysis	684:703	optimized hydrolysis	684:703	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	1	57	theme	medicinal	147:155	arg1	fungus					157:162	a well-known and important medicinal fungus	120:162	a well-known and important medicinal fungus in Asia	120:170	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	1	57	theme	medicinal	147:155	arg1	umbellatus					106:115	Polyporus umbellatus	96:115	Polyporus umbellatus	96:115	Polyporus umbellatus is a well-known and important medicinal fungus in Asia.					
31295903	7	58	theme	monosaccharides	1161:1175	arg1	behaviors					1074:1082	mass spectral behaviors	1060:1082	mass spectral behaviors	1060:1082	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	7	58	theme	monosaccharides	1161:1175	arg1	characteristics					1103:1117	chromatography characteristics	1088:1117	chromatography characteristics	1088:1117	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	5	59	theme	mol/L	822:826	arg1	TFA					828:830	3 mol/L TFA	820:830	3 mol/L TFA	820:830	The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C.					
31295903	4	60	theme	polysaccharides	616:630	arg1	conditions					598:607	The hydrolysis conditions	583:607	The hydrolysis conditions of the polysaccharides	583:630	The hydrolysis conditions of the polysaccharides were optimized by orthogonal design.					
31295903	2	61	theme	immunomodulatory	280:295	arg1	effects					297:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	antitumor, antioxidant, hepatoprotective and immunomodulatory effects	235:303	Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects.					
31295903	7	62	theme	mass	1060:1063	arg1	behaviors					1074:1082	mass spectral behaviors	1060:1082	mass spectral behaviors	1060:1082	Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides.					
31295903	10	63	theme	natural	1673:1679	arg1	method					1499:1504	This method	1494:1504	This method	1494:1504	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
31295903	10	63	theme	natural	1673:1679	arg1	medicine					1681:1688	a natural medicine	1671:1688	a natural medicine	1671:1688	This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.					
30914368	9	0	theme	fermented	1259:1267	arg1	barley					1269:1274	fermented barley	1259:1274	fermented barley	1259:1274	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	7	1	theme	RAW264.7	915:922	arg1	cells					924:928	RAW264.7 cells	915:928	RAW264.7 cells	915:928	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	9	2	theme	several	1378:1384	arg1	TLR4					1427:1430	TLR4	1427:1430	TLR4	1427:1430	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	2	theme	several	1378:1384	arg1	dectin-1					1417:1424	dectin-1	1417:1424	dectin-1	1417:1424	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	2	theme	several	1378:1384	arg1	receptors					1397:1405	several macrophage receptors	1378:1405	several macrophage receptors including dectin-1, TLR4, SR, and CD14	1378:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	2	theme	several	1378:1384	arg1	SR					1433:1434	SR	1433:1434	SR	1433:1434	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	2	theme	several	1378:1384	arg1	CD14					1441:1444	CD14	1441:1444	CD14	1441:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	3	3	theme	fermented	359:367	arg1	barley					369:374	fermented barley	359:374	fermented barley	359:374	In this study, three polysaccharide fractions (BF-I-III) were purified from fermented barley and their monosaccharide composition was analyzed.					
30914368	5	4	theme	IL-12	663:667	arg1	production					643:652	the production	639:652	the production of IL-6, IL-12, and TNF-α	639:678	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	9	5	attach	isolated	1245:1252	arg1	barley					1269:1274	fermented barley	1259:1274	fermented barley	1259:1274	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	5	attach	isolated	1245:1252	arg2	BF-I					1239:1242	BF-I	1239:1242	BF-I	1239:1242	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	4	6	theme	RAW264.7	521:528	arg1	cells					530:534	RAW264.7 cells	521:534	RAW264.7 cells	521:534	Their immune-stimulatory activities and intracellular signaling pathways were also studied in RAW264.7 cells.					
30914368	7	7	theme	p65	908:910	arg1	translocation					891:903	the nuclear translocation	879:903	the nuclear translocation of p65 in RAW264.7 cells	879:928	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	8	8	theme	production	1129:1138	arg1	stimulation					1096:1106	the stimulation	1092:1106	the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation	1092:1208	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	4	9	theme	signaling	481:489	arg1	pathways					491:498	intracellular signaling pathways	467:498	intracellular signaling pathways	467:498	Their immune-stimulatory activities and intracellular signaling pathways were also studied in RAW264.7 cells.					
30914368	7	10	theme	nuclear	883:889	arg1	translocation					891:903	the nuclear translocation	879:903	the nuclear translocation of p65 in RAW264.7 cells	879:928	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	8	11	theme	oxide	1118:1122	arg1	production					1129:1138	nitric oxide (NO) production	1111:1138	nitric oxide (NO) production	1111:1138	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	8	12	dep	receptor	1044:1051	arg1	scavenge					1035:1042	scavenge	1035:1042	scavenge	1035:1042	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	5	13	theme	TNF-α	674:678	arg1	production					643:652	the production	639:652	the production of IL-6, IL-12, and TNF-α	639:678	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	3	14	theme	monosaccharide	386:399	arg1	composition					401:411	their monosaccharide composition	380:411	their monosaccharide composition	380:411	In this study, three polysaccharide fractions (BF-I-III) were purified from fermented barley and their monosaccharide composition was analyzed.					
30914368	9	15	theme	immune	1284:1289	arg1	activity					1304:1311	an immune potentiation activity	1281:1311	an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14	1281:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	2	16	attach	derived	252:258	arg1	barley					275:280	fermented barley	265:280	fermented barley	265:280	We have previously demonstrated the macrophage-stimulating properties of polysaccharides derived from fermented barley.					
30914368	2	16	attach	derived	252:258	arg2	polysaccharides					236:250	polysaccharides	236:250	polysaccharides derived from fermented barley	236:280	We have previously demonstrated the macrophage-stimulating properties of polysaccharides derived from fermented barley.					
30914368	9	17	theme	potentiation	1291:1302	arg1	activity					1304:1311	an immune potentiation activity	1281:1311	an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14	1281:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	18	from	activity	1304:1311	arg1	macrophages					1316:1326	macrophages	1316:1326	macrophages	1316:1326	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	5	19	theme	enhanced	579:586	arg1	properties					610:619	enhanced macrophage activation properties	579:619	enhanced macrophage activation properties	579:619	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	0	20	theme	Signaling	0:8	arg1	pathways					10:17	Signaling pathways	0:17	Signaling pathways	0:17	Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley.					
30914368	4	21	theme	immune-stimulatory	433:450	arg1	activities					452:461	Their immune-stimulatory activities	427:461	Their immune-stimulatory activities	427:461	Their immune-stimulatory activities and intracellular signaling pathways were also studied in RAW264.7 cells.					
30914368	6	22	from	effects	723:729	arg1	cells					744:748	RAW 264.7 cells	734:748	RAW 264.7 cells	734:748	However, BF-II and BF-III showed moderate effects on RAW 264.7 cells.					
30914368	8	23	with	experiments	944:954	arg1	antibodies					974:983	neutralizing antibodies	961:983	neutralizing antibodies	961:983	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	5	24	theme	IL-6	657:660	arg1	production					643:652	the production	639:652	the production of IL-6, IL-12, and TNF-α	639:678	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	4	25	theme	intracellular	467:479	arg1	pathways					491:498	intracellular signaling pathways	467:498	intracellular signaling pathways	467:498	Their immune-stimulatory activities and intracellular signaling pathways were also studied in RAW264.7 cells.					
30914368	6	26	theme	RAW	734:736	arg1	cells					744:748	RAW 264.7 cells	734:748	RAW 264.7 cells	734:748	However, BF-II and BF-III showed moderate effects on RAW 264.7 cells.					
30914368	7	27	theme	major	821:825	arg1	component					827:835	major component	821:835	major component of AP-1 transcription factor	821:864	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	2	28	theme	macrophage-stimulating	199:220	arg1	properties					222:231	the macrophage-stimulating properties	195:231	the macrophage-stimulating properties of polysaccharides derived from fermented barley	195:280	We have previously demonstrated the macrophage-stimulating properties of polysaccharides derived from fermented barley.					
30914368	9	29	contain	has	1277:1279	arg1	BF-I					1239:1242	BF-I	1239:1242	BF-I	1239:1242	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	29	contain	has	1277:1279	arg2	activity					1304:1311	an immune potentiation activity	1281:1311	an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14	1281:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	7	30	theme	transcription	845:857	arg1	factor					859:864	AP-1 transcription factor	840:864	AP-1 transcription factor	840:864	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	8	31	theme	toll-like	1007:1015	arg1	TLR					1027:1029	toll-like receptor (TLR) 4	1007:1032	toll-like receptor (TLR) 4	1007:1032	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	7	32	theme	factor	859:864	arg1	component					827:835	major component	821:835	major component of AP-1 transcription factor	821:864	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	0	33	theme	macrophage-activating	35:55	arg1	polysaccharides					57:71	macrophage-activating polysaccharides	35:71	macrophage-activating polysaccharides purified from fermented barley	35:102	Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley.					
30914368	3	34	theme	polysaccharide	304:317	arg1	fractions					319:327	three polysaccharide fractions	298:327	three polysaccharide fractions (BF-I-III)	298:338	In this study, three polysaccharide fractions (BF-I-III) were purified from fermented barley and their monosaccharide composition was analyzed.					
30914368	3	34	theme	polysaccharide	304:317	arg1	BF-I-III					330:337	BF-I-III	330:337	BF-I-III	330:337	In this study, three polysaccharide fractions (BF-I-III) were purified from fermented barley and their monosaccharide composition was analyzed.					
30914368	8	35	theme	phosphorylation	1194:1208	arg1	inhibition					1176:1185	the inhibition	1172:1185	the inhibition of JNK phosphorylation	1172:1208	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	7	36	theme	AP-1	840:843	arg1	factor					859:864	AP-1 transcription factor	840:864	AP-1 transcription factor	840:864	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	2	37	theme	fermented	265:273	arg1	barley					275:280	fermented barley	265:280	fermented barley	265:280	We have previously demonstrated the macrophage-stimulating properties of polysaccharides derived from fermented barley.					
30914368	8	38	theme	nitric	1111:1116	arg1	NO					1125:1126	NO	1125:1126	NO	1125:1126	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	8	38	theme	nitric	1111:1116	arg1	oxide					1118:1122	nitric oxide	1111:1122	nitric oxide (NO) production	1111:1138	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	1	39	theme	many	132:135	arg1	food					137:140	many food	132:140	many food	132:140	Barley is commonly used in many food and health products.					
30914368	8	40	theme	JNK	1190:1192	arg1	phosphorylation					1194:1208	JNK phosphorylation	1190:1208	JNK phosphorylation	1190:1208	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	7	41	dep	led	766:768	arg1	component					827:835	major component	821:835	major component of AP-1 transcription factor	821:864	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	8	42	theme	neutralizing	961:972	arg1	antibodies					974:983	neutralizing antibodies	961:983	neutralizing antibodies	961:983	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	1	43	used	used	124:127	arg2	Barley					105:110	Barley	105:110	Barley	105:110	Barley is commonly used in many food and health products.					
30914368	6	44	theme	moderate	714:721	arg1	effects					723:729	moderate effects	714:729	moderate effects on RAW 264.7 cells	714:748	However, BF-II and BF-III showed moderate effects on RAW 264.7 cells.					
30914368	7	45	theme	BF-I	751:754	arg1	treatment					756:764	BF-I treatment	751:764	BF-I treatment	751:764	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	2	46	theme	polysaccharides	236:250	arg1	properties					222:231	the macrophage-stimulating properties	195:231	the macrophage-stimulating properties of polysaccharides derived from fermented barley	195:280	We have previously demonstrated the macrophage-stimulating properties of polysaccharides derived from fermented barley.					
30914368	8	47	theme	receptor	1017:1024	arg1	TLR					1027:1029	toll-like receptor (TLR) 4	1007:1032	toll-like receptor (TLR) 4	1007:1032	In addition, experiments with neutralizing antibodies showed that Dectin-1, toll-like receptor (TLR) 4, scavenge receptor (SR), and CD14 were mainly involved in the stimulation of nitric oxide (NO) production by BF-I which was suppressed by the inhibition of JNK phosphorylation.					
30914368	9	48	theme	JNK	1352:1354	arg1	pathway					1366:1372	the JNK signaling pathway	1348:1372	the JNK signaling pathway	1348:1372	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	49	theme	macrophage	1386:1395	arg1	TLR4					1427:1430	TLR4	1427:1430	TLR4	1427:1430	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	49	theme	macrophage	1386:1395	arg1	dectin-1					1417:1424	dectin-1	1417:1424	dectin-1	1417:1424	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	49	theme	macrophage	1386:1395	arg1	receptors					1397:1405	several macrophage receptors	1378:1405	several macrophage receptors including dectin-1, TLR4, SR, and CD14	1378:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	49	theme	macrophage	1386:1395	arg1	SR					1433:1434	SR	1433:1434	SR	1433:1434	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	9	49	theme	macrophage	1386:1395	arg1	CD14					1441:1444	CD14	1441:1444	CD14	1441:1444	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	5	50	theme	macrophage	588:597	arg1	properties					610:619	enhanced macrophage activation properties	579:619	enhanced macrophage activation properties	579:619	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	1	51	theme	health	146:151	arg1	products					153:160	health products	146:160	health products	146:160	Barley is commonly used in many food and health products.					
30914368	9	52	theme	signaling	1356:1364	arg1	pathway					1366:1372	the JNK signaling pathway	1348:1372	the JNK signaling pathway	1348:1372	These findings suggest that BF-I, isolated from fermented barley, has an immune potentiation activity on macrophages, where it activates the JNK signaling pathway via several macrophage receptors including dectin-1, TLR4, SR, and CD14.					
30914368	5	53	theme	activation	599:608	arg1	properties					610:619	enhanced macrophage activation properties	579:619	enhanced macrophage activation properties	579:619	Among the three fractions, BF-I exhibited enhanced macrophage activation properties, such as inducing the production of IL-6, IL-12, and TNF-α.					
30914368	0	54	theme	fermented	87:95	arg1	barley					97:102	fermented barley	87:102	fermented barley	87:102	Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley.					
30914368	7	55	from	translocation	891:903	arg1	cells					924:928	RAW264.7 cells	915:928	RAW264.7 cells	915:928	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	7	56	theme	MAPKs	796:800	arg1	c-Jun					814:818	c-Jun	814:818	c-Jun	814:818	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	7	56	theme	MAPKs	796:800	arg1	phosphorylation					777:791	the phosphorylation	773:791	the phosphorylation of MAPKs	773:800	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
30914368	7	56	theme	MAPKs	796:800	arg1	NF-κB					803:807	NF-κB	803:807	NF-κB	803:807	BF-I treatment led to the phosphorylation of MAPKs, NF-κB, and c-Jun (major component of AP-1 transcription factor) and induced the nuclear translocation of p65 in RAW264.7 cells.					
29679870	2	0	theme	matter	426:431	arg1	removal					380:386	the simultaneous removal	363:386	the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	363:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	3	1	theme	coagulation	619:629	arg1	process					631:637	this coagulation process	614:637	this coagulation process	614:637	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	9	2	with	flocs	1325:1329	arg1	recoverability					1343:1356	better recoverability	1336:1356	better recoverability	1336:1356	Smaller and denser flocs with better recoverability were formed as the HA concentration increased.					
29679870	7	3	theme	Cu2+	1107:1110	arg1	both					1117:1120	both	1117:1120	both	1117:1120	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	3	theme	Cu2+	1107:1110	arg1	efficiencies					1079:1090	the removal efficiencies	1067:1090	the removal efficiencies of CuO NPs and Cu2+	1067:1110	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	2	4	theme	organic	418:424	arg1	NOM					434:436	NOM	434:436	NOM	434:436	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	2	4	theme	organic	418:424	arg1	matter					426:431	natural organic matter	410:431	natural organic matter (NOM)	410:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	12	5	from	Ep	1846:1847	arg1	species					1863:1869	species	1863:1869	Al (III) species (from PAC)	1854:1880	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	12	5	from	Ep	1846:1847	arg1	groups					1833:1838	carboxyl and hydroxyl groups	1811:1838	groups	1833:1838	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	1	6	theme	Humic	156:160	arg1	HA					168:169	HA	168:169	HA	168:169	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	1	6	theme	Humic	156:160	arg1	acid					162:165	Humic acid	156:165	Humic acid (HA)	156:170	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	12	7	theme	reticular	1758:1766	arg1	structure					1768:1776	a chelated reticular structure	1747:1776	a chelated reticular structure	1747:1776	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	0	8	theme	nanoparticles	96:108	arg1	removal					81:87	the simultaneous removal	64:87	the simultaneous removal of CuO nanoparticles	64:108	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	0	8	theme	nanoparticles	96:108	arg1	Cu2+					114:117	Cu2+	114:117	Cu2+	114:117	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	12	9	theme	chelated	1749:1756	arg1	structure					1768:1776	a chelated reticular structure	1747:1776	a chelated reticular structure	1747:1776	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	3	10	theme	novel	499:503	arg1	coagulant					515:523	the novel recycling coagulant	495:523	the novel recycling coagulant aid	495:527	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	3	10	theme	novel	499:503	arg1	polysaccharides					470:484	Enteromorpha polysaccharides	457:484	Enteromorpha polysaccharides (Ep)	457:489	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	9	11	theme	better	1336:1341	arg1	recoverability					1343:1356	better recoverability	1336:1356	better recoverability	1336:1356	Smaller and denser flocs with better recoverability were formed as the HA concentration increased.					
29679870	10	12	theme	composite	1557:1565	arg1	contaminant					1567:1577	a composite contaminant	1555:1577	a composite contaminant	1555:1577	Furthermore, because of affluent functional groups, HA was easily adsorbed on the surface of NPs and combined with dissociative Cu2+, thereby forming a composite contaminant.					
29679870	0	13	theme	CuO	92:94	arg1	nanoparticles					96:108	CuO nanoparticles	92:108	CuO nanoparticles	92:108	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	10	14	theme	dissociative	1520:1531	arg1	Cu2+					1533:1536	dissociative Cu2+	1520:1536	dissociative Cu2+	1520:1536	Furthermore, because of affluent functional groups, HA was easily adsorbed on the surface of NPs and combined with dissociative Cu2+, thereby forming a composite contaminant.					
29679870	4	15	theme	zeta	678:681	arg1	potential					683:691	zeta potential	678:691	zeta potential	678:691	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	9	16	theme	Smaller	1306:1312	arg1	flocs					1325:1329	Smaller and denser flocs	1306:1329	Smaller and denser flocs with better recoverability	1306:1356	Smaller and denser flocs with better recoverability were formed as the HA concentration increased.					
29679870	12	17	dep	species	1863:1869	arg1	III					1858:1860	III	1858:1860	III	1858:1860	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	12	17	dep	species	1863:1869	arg1	Al					1854:1855	Al (III) species (from PAC)	1854:1880	Al (III) species (from PAC)	1854:1880	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	11	18	theme	coagulation	1591:1601	arg1	process					1603:1609	the coagulation process	1587:1609	the coagulation process	1587:1609	During the coagulation process, the colloidal system was destabilized preferentially by the charge neutralization between composite contaminants and PAC.					
29679870	3	19	theme	Enteromorpha	457:468	arg1	coagulant					515:523	the novel recycling coagulant	495:523	the novel recycling coagulant aid	495:527	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	3	19	theme	Enteromorpha	457:468	arg1	Ep					487:488	Ep	487:488	Ep	487:488	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	3	19	theme	Enteromorpha	457:468	arg1	polysaccharides					470:484	Enteromorpha polysaccharides	457:484	Enteromorpha polysaccharides (Ep)	457:489	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	2	20	theme	concentration	346:358	arg1	impact					333:338	the impact	329:338	the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	329:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	12	21	theme	hydroxyl	1824:1831	arg1	groups					1833:1838	carboxyl and hydroxyl groups	1811:1838	groups	1833:1838	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	1	22	from	performance	265:275	arg1	coagulation					287:297	coagulation	287:297	coagulation	287:297	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	7	23	theme	removal	1071:1077	arg1	both					1117:1120	both	1117:1120	both	1117:1120	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	23	theme	removal	1071:1077	arg1	efficiencies					1079:1090	the removal efficiencies	1067:1090	the removal efficiencies of CuO NPs and Cu2+	1067:1110	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	8	24	theme	Cu	1285:1286	arg1	efficiency					1271:1280	the removal efficiency	1259:1280	the removal efficiency of Cu	1259:1286	However, excessive HA (more than 10 mg/L) reduced the removal efficiency of Cu by more than 31%.					
29679870	2	25	theme	HA	343:344	arg1	concentration					346:358	HA concentration	343:358	HA concentration	343:358	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	7	26	theme	CuO	1095:1097	arg1	NPs					1099:1101	CuO NPs	1095:1101	CuO NPs	1095:1101	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	4	27	dep	Fourier	741:747	arg1	Transform					749:757	Transform	749:757	Transform Infrared Spectra (FT-IR)	749:782	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	4	28	theme	floc	661:664	arg1	properties					666:675	floc properties	661:675	floc properties	661:675	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	3	29	dep	coagulant	515:523	arg1	aid					525:527	aid	525:527	aid	525:527	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	2	30	theme	NPs	395:397	arg1	removal					380:386	the simultaneous removal	363:386	the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	363:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	4	31	theme	Infrared	759:766	arg1	FT-IR					777:781	FT-IR	777:781	FT-IR	777:781	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	4	31	theme	Infrared	759:766	arg1	Spectra					768:774	Infrared Spectra	759:774	Infrared Spectra (FT-IR)	759:782	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	3	32	from	contaminants	598:609	arg1	process					631:637	this coagulation process	614:637	this coagulation process	614:637	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	7	33	from	%	1206:1206	arg1	particular					1144:1153	particular	1144:1153	particular	1144:1153	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	34	theme	highest	1160:1166	arg1	%					1206:1206	98%	1204:1206	98%	1204:1206	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	34	theme	highest	1160:1166	arg1	efficiency					1176:1185	the highest removal efficiency	1156:1185	the highest removal efficiency of turbidity	1156:1198	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	0	35	theme	polysaccharides	28:42	arg1	Application					0:10	Application	0:10	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.	0:154	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	12	36	dep	groups	1833:1838	arg1	the					1792:1794	the	1792:1794	the	1792:1794	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	12	36	dep	groups	1833:1838	arg1	conjunction					1796:1806	conjunction	1796:1806	conjunction	1796:1806	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	12	37	from	PAC	1877:1879	arg1	species					1863:1869	species	1863:1869	Al (III) species (from PAC)	1854:1880	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	12	37	from	PAC	1877:1879	arg1	groups					1833:1838	carboxyl and hydroxyl groups	1811:1838	groups	1833:1838	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	7	38	dep	both	1117:1120	arg1	%					1134:1134	%	1134:1134	%	1134:1134	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	6	39	theme	coagulation	937:947	arg1	performance					949:959	the coagulation performance	933:959	the coagulation performance	933:959	Further, the coagulation performance became better with the increase in HA concentration.					
29679870	0	40	theme	enteromorpha	15:26	arg1	polysaccharides					28:42	enteromorpha polysaccharides	15:42	enteromorpha polysaccharides	15:42	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	1	41	theme	removal	257:263	arg1	performance					265:275	the removal performance	253:275	the removal performance of NPs in coagulation	253:297	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	4	42	theme	scanning	694:701	arg1	SEM					724:726	SEM	724:726	SEM	724:726	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	4	42	theme	scanning	694:701	arg1	microscope					712:721	scanning electron microscope	694:721	scanning electron microscope (SEM) images	694:734	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	8	43	theme	removal	1263:1269	arg1	efficiency					1271:1280	the removal efficiency	1259:1280	the removal efficiency of Cu	1259:1286	However, excessive HA (more than 10 mg/L) reduced the removal efficiency of Cu by more than 31%.					
29679870	7	44	dep	%	1134:1134	arg1	80					1132:1133	80	1132:1133	80	1132:1133	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	12	45	theme	carboxyl	1811:1818	arg1	groups					1833:1838	carboxyl and hydroxyl groups	1811:1838	groups	1833:1838	Furthermore, a chelated reticular structure was formed by the conjunction of carboxyl and hydroxyl groups (from Ep) and Al (III) species (from PAC).					
29679870	2	46	theme	simultaneous	367:378	arg1	removal					380:386	the simultaneous removal	363:386	the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	363:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	2	47	theme	natural	410:416	arg1	NOM					434:436	NOM	434:436	NOM	434:436	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	2	47	theme	natural	410:416	arg1	matter					426:431	natural organic matter	410:431	natural organic matter (NOM)	410:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	10	48	theme	NPs	1498:1500	arg1	surface					1487:1493	the surface	1483:1493	the surface of NPs	1483:1500	Furthermore, because of affluent functional groups, HA was easily adsorbed on the surface of NPs and combined with dissociative Cu2+, thereby forming a composite contaminant.					
29679870	0	49	theme	humic	130:134	arg1	concentration					141:153	humic acid concentration	130:153	humic acid concentration	130:153	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	11	50	theme	composite	1702:1710	arg1	contaminants					1712:1723	composite contaminants	1702:1723	composite contaminants	1702:1723	During the coagulation process, the colloidal system was destabilized preferentially by the charge neutralization between composite contaminants and PAC.					
29679870	7	51	theme	removal	1168:1174	arg1	%					1206:1206	98%	1204:1206	98%	1204:1206	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	51	theme	removal	1168:1174	arg1	efficiency					1176:1185	the highest removal efficiency	1156:1185	the highest removal efficiency of turbidity	1156:1198	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	11	52	theme	charge	1672:1677	arg1	neutralization					1679:1692	the charge neutralization	1668:1692	the charge neutralization between composite contaminants and PAC	1668:1731	During the coagulation process, the colloidal system was destabilized preferentially by the charge neutralization between composite contaminants and PAC.					
29679870	10	53	theme	affluent	1429:1436	arg1	groups					1449:1454	affluent functional groups	1429:1454	affluent functional groups	1429:1454	Furthermore, because of affluent functional groups, HA was easily adsorbed on the surface of NPs and combined with dissociative Cu2+, thereby forming a composite contaminant.					
29679870	3	54	theme	polyaluminum	551:562	arg1	PAC					574:576	PAC	574:576	PAC	574:576	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	3	54	theme	polyaluminum	551:562	arg1	chloride					564:571	polyaluminum chloride	551:571	polyaluminum chloride (PAC)	551:577	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	2	55	theme	CuO	391:393	arg1	NPs					395:397	CuO NPs	391:397	CuO NPs	391:397	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	11	56	theme	colloidal	1616:1624	arg1	system					1626:1631	the colloidal system	1612:1631	the colloidal system	1612:1631	During the coagulation process, the colloidal system was destabilized preferentially by the charge neutralization between composite contaminants and PAC.					
29679870	5	57	with	PAC	832:834	arg1	PAC-Ep					845:850	PAC-Ep	845:850	PAC-Ep	845:850	Results showed that PAC with Ep (PAC-Ep) was more beneficial for improving removal performances than PAC alone.					
29679870	5	57	with	PAC	832:834	arg1	Ep					841:842	Ep	841:842	Ep (PAC-Ep)	841:851	Results showed that PAC with Ep (PAC-Ep) was more beneficial for improving removal performances than PAC alone.					
29679870	1	58	theme	NPs	280:282	arg1	performance					265:275	the removal performance	253:275	the removal performance of NPs in coagulation	253:297	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	0	59	theme	concentration	141:153	arg1	Effect					120:125	Effect	120:125	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.	0:154	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	0	60	dep	coagulant	47:55	arg1	aid					57:59	aid	57:59	aid in the simultaneous removal of CuO nanoparticles and Cu2+	57:117	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	2	61	from	impact	333:338	arg1	removal					380:386	the simultaneous removal	363:386	the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	363:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	8	62	dep	reduced	1251:1257	arg1	more					1232:1235	more	1232:1235	more	1232:1235	However, excessive HA (more than 10 mg/L) reduced the removal efficiency of Cu by more than 31%.					
29679870	2	63	theme	Cu2+	400:403	arg1	removal					380:386	the simultaneous removal	363:386	the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM)	363:437	Consequently, in this study, the impact of HA concentration on the simultaneous removal of CuO NPs, Cu2+, and natural organic matter (NOM) was investigated.					
29679870	0	64	theme	acid	136:139	arg1	concentration					141:153	humic acid concentration	130:153	humic acid concentration	130:153	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	3	65	theme	recycling	505:513	arg1	coagulant					515:523	the novel recycling coagulant	495:523	the novel recycling coagulant aid	495:527	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	3	65	theme	recycling	505:513	arg1	polysaccharides					470:484	Enteromorpha polysaccharides	457:484	Enteromorpha polysaccharides (Ep)	457:489	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	7	66	theme	NPs	1099:1101	arg1	both					1117:1120	both	1117:1120	both	1117:1120	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	66	theme	NPs	1099:1101	arg1	efficiencies					1079:1090	the removal efficiencies	1067:1090	the removal efficiencies of CuO NPs and Cu2+	1067:1110	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	67	theme	turbidity	1190:1198	arg1	%					1206:1206	98%	1204:1206	98%	1204:1206	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	67	theme	turbidity	1190:1198	arg1	efficiency					1176:1185	the highest removal efficiency	1156:1185	the highest removal efficiency of turbidity	1156:1198	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	10	68	theme	functional	1438:1447	arg1	groups					1449:1454	affluent functional groups	1429:1454	affluent functional groups	1429:1454	Furthermore, because of affluent functional groups, HA was easily adsorbed on the surface of NPs and combined with dissociative Cu2+, thereby forming a composite contaminant.					
29679870	3	69	theme	composite	588:596	arg1	contaminants					598:609	composite contaminants	588:609	composite contaminants in this coagulation process	588:637	Enteromorpha polysaccharides (Ep), as the novel recycling coagulant aid, were integrated with polyaluminum chloride (PAC) to treat composite contaminants in this coagulation process.					
29679870	1	70	dep	aggregation	187:197	arg1	the					183:185	the	183:185	the	183:185	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	8	71	theme	excessive	1218:1226	arg1	HA					1228:1229	excessive HA	1218:1229	excessive HA	1218:1229	However, excessive HA (more than 10 mg/L) reduced the removal efficiency of Cu by more than 31%.					
29679870	9	72	theme	HA	1377:1378	arg1	concentration					1380:1392	the HA concentration	1373:1392	the HA concentration	1373:1392	Smaller and denser flocs with better recoverability were formed as the HA concentration increased.					
29679870	4	73	theme	electron	703:710	arg1	SEM					724:726	SEM	724:726	SEM	724:726	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	4	73	theme	electron	703:710	arg1	microscope					712:721	scanning electron microscope	694:721	scanning electron microscope (SEM) images	694:734	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	6	74	from	increase	984:991	arg1	concentration					999:1011	HA concentration	996:1011	HA concentration	996:1011	Further, the coagulation performance became better with the increase in HA concentration.					
29679870	4	75	theme	Removal	640:646	arg1	performance					648:658	Removal performance	640:658	Removal performance	640:658	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	4	76	theme	microscope	712:721	arg1	images					729:734	scanning electron microscope (SEM) images	694:734	scanning electron microscope (SEM) images	694:734	Removal performance, floc properties, zeta potential, scanning electron microscope (SEM) images, and Fourier Transform Infrared Spectra (FT-IR) were measured and analyzed.					
29679870	0	77	theme	simultaneous	68:79	arg1	removal					81:87	the simultaneous removal	64:87	the simultaneous removal of CuO nanoparticles	64:108	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	5	78	theme	removal	887:893	arg1	performances					895:906	removal performances	887:906	removal performances than PAC alone	887:921	Results showed that PAC with Ep (PAC-Ep) was more beneficial for improving removal performances than PAC alone.					
29679870	6	79	theme	HA	996:997	arg1	concentration					999:1011	HA concentration	996:1011	HA concentration	996:1011	Further, the coagulation performance became better with the increase in HA concentration.					
29679870	1	80	theme	nanoparticles	216:228	arg1	stability					203:211	stability	203:211	stability	203:211	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	1	80	theme	nanoparticles	216:228	arg1	aggregation					187:197	aggregation	187:197	aggregation	187:197	Humic acid (HA) influences the aggregation and stability of nanoparticles (NPs), which determine the removal performance of NPs in coagulation.					
29679870	7	81	theme	HA	1023:1024	arg1	10 mg/L					1044:1050	10 mg/L	1044:1050	10 mg/L	1044:1050	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	7	81	theme	HA	1023:1024	arg1	concentration					1026:1038	the HA concentration	1019:1038	the HA concentration	1019:1038	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29679870	0	82	dep	Application	0:10	arg1	Effect					120:125	Effect	120:125	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.	0:154	Application of enteromorpha polysaccharides as coagulant aid in the simultaneous removal of CuO nanoparticles and Cu2+: Effect of humic acid concentration.					
29679870	9	83	theme	denser	1318:1323	arg1	flocs					1325:1329	Smaller and denser flocs	1306:1329	Smaller and denser flocs with better recoverability	1306:1356	Smaller and denser flocs with better recoverability were formed as the HA concentration increased.					
29679870	7	84	dep	10 mg/L	1044:1050	arg1	used					1053:1056	used	1053:1056	used PAC-Ep	1053:1063	When the HA concentration was 10 mg/L (used PAC-Ep), the removal efficiencies of CuO NPs and Cu2+ were both more than 80%; and in particular, the highest removal efficiency of turbidity was 98%.					
29932998	0	0	theme	drug	112:115	arg1	delivery					117:124	intragastric drug delivery	99:124	intragastric drug delivery	99:124	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	6	1	theme	release	1024:1030	arg1	behaviours					1032:1041	extended drug release behaviours	1010:1041	extended drug release behaviours (Q8h, 69-94%)	1010:1055	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	7	2	theme	oil-entrapped	1099:1111	arg1	NF/GG					1123:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	6	3	dep	behaviours	1032:1041	arg1	Q8h					1044:1046	Q8h	1044:1046	Q8h	1044:1046	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	6	3	dep	behaviours	1032:1041	arg1	%					1054:1054	69-94%	1049:1054	69-94%	1049:1054	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	7	4	theme	drug	1202:1205	arg1	release					1207:1213	its drug release	1198:1213	its drug release	1198:1213	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	6	5	theme	extended	1010:1017	arg1	behaviours					1032:1041	extended drug release behaviours	1010:1041	extended drug release behaviours (Q8h, 69-94%)	1010:1055	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	0	6	theme	intragastric	99:110	arg1	delivery					117:124	intragastric drug delivery	99:124	intragastric drug delivery	99:124	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	7	7	theme	Florite	1113:1119	arg1	NF/GG					1123:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	6	8	theme	GG	852:853	arg1	ratios					842:847	the mass ratios	833:847	the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion	833:925	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	1	9	theme	-gellan	206:212	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	9	theme	-gellan	206:212	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	9	10	theme	MFM	1511:1513	arg1	delivery					1499:1506	controlled intragastric delivery	1475:1506	controlled intragastric delivery of MFM for the management of type 2 diabetes	1475:1551	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	1	11	theme	metformin	303:311	arg1	MFM					318:320	MFM	318:320	MFM	318:320	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	11	theme	metformin	303:311	arg1	HCl					313:315	metformin HCl	303:315	metformin HCl (MFM)	303:321	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	4	12	from	environment	620:630	arg1	composites					639:648	the composites	635:648	the composites compatible with the drug	635:673	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	9	13	theme	type	1537:1540	arg1	diabetes					1544:1551	type 2 diabetes	1537:1551	type 2 diabetes	1537:1551	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	7	14	theme	transport	1174:1182	arg1	mechanism					1184:1192	case-II transport mechanism	1166:1192	case-II transport mechanism	1166:1192	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	1	15	theme	HCl	313:315	arg1	delivery					291:298	controlled intragastric delivery	267:298	controlled intragastric delivery of metformin HCl (MFM)	267:321	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	7	16	theme	case-II	1166:1172	arg1	mechanism					1184:1192	case-II transport mechanism	1166:1192	case-II transport mechanism	1166:1192	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	6	17	theme	inclusion	917:925	arg1	types					860:864	types	860:864	types of nanofiller (neusilin/bentonite/Florite) and oil inclusion	860:925	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	6	17	theme	inclusion	917:925	arg1	GG					852:853	GG	852:853	GG	852:853	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	7	18	theme	optimized	1089:1097	arg1	NF/GG					1123:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	6	19	theme	drug	962:965	arg1	efficiency					981:990	variable drug encapsulation efficiency	953:990	variable drug encapsulation efficiency (DEE, 50-85%)	953:1004	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	8	20	theme	excellent	1304:1312	arg1	properties					1330:1339	excellent gastroretentive properties	1304:1339	excellent gastroretentive properties	1304:1339	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	9	21	theme	controlled	1475:1484	arg1	delivery					1499:1506	controlled intragastric delivery	1475:1506	controlled intragastric delivery of MFM for the management of type 2 diabetes	1475:1551	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	2	22	theme	amidation	344:352	arg1	degree					334:339	a degree	332:339	a degree of amidation of 48.7%	332:361	DMP had a degree of amidation of 48.7% and was characterized further by FTIR, XRD and DSC analyses.					
29932998	2	23	theme	%	361:361	arg1	amidation					344:352	amidation	344:352	amidation of 48.7%	344:361	DMP had a degree of amidation of 48.7% and was characterized further by FTIR, XRD and DSC analyses.					
29932998	6	24	theme	mass	837:840	arg1	ratios					842:847	the mass ratios	833:847	the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion	833:925	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	8	25	theme	gastroretentive	1314:1328	arg1	properties					1330:1339	excellent gastroretentive properties	1304:1339	excellent gastroretentive properties	1304:1339	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	4	26	from	degree	691:696	arg1	crystallinity					723:735	drug's crystallinity	716:735	drug's crystallinity	716:735	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	1	27	theme	gum	214:216	arg1	composites					237:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	5	28	theme	spherical	783:791	arg1	shape					793:797	almost spherical shape	776:797	almost spherical shape of the composites	776:815	Scanning electron microscopy revealed almost spherical shape of the composites.					
29932998	7	29	dep	NF/GG	1123:1127	arg1	data					1215:1218	data	1215:1218	data	1215:1218	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	7	30	theme	R	1121:1121	arg1	NF/GG					1123:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG	1085:1127	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	3	31	theme	gelation	511:518	arg1	technique					520:528	ionotropic gelation technique	500:528	ionotropic gelation technique using zinc acetate as cross-linker	500:563	MFM-loaded composites were subsequently accomplished by green synthesis via ionotropic gelation technique using zinc acetate as cross-linker.					
29932998	6	32	theme	acetate	1060:1066	arg1	pH 4.5					1076:1081	pH 4.5	1076:1081	pH 4.5	1076:1081	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	6	32	theme	acetate	1060:1066	arg1	buffer					1068:1073	acetate buffer	1060:1073	acetate buffer (pH 4.5)	1060:1082	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	5	33	theme	composites	806:815	arg1	shape					793:797	almost spherical shape	776:797	almost spherical shape of the composites	776:815	Scanning electron microscopy revealed almost spherical shape of the composites.					
29932998	6	34	theme	oil	913:915	arg1	inclusion					917:925	oil inclusion	913:925	oil inclusion	913:925	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	3	35	theme	MFM-loaded	424:433	arg1	composites					435:444	MFM-loaded composites	424:444	MFM-loaded composites	424:444	MFM-loaded composites were subsequently accomplished by green synthesis via ionotropic gelation technique using zinc acetate as cross-linker.					
29932998	0	36	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	4	37	theme	X-ray	579:583	arg1	analyses					598:605	The thermal, X-ray and infrared analyses	566:605	analyses	598:605	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	8	38	theme	hypoglycemic	1357:1368	arg1	effect					1370:1375	substantial hypoglycemic effect	1345:1375	substantial hypoglycemic effect	1345:1375	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	8	39	theme	streptozotocin-induced	1380:1401	arg1	rats					1412:1415	streptozotocin-induced diabetic rats	1380:1415	streptozotocin-induced diabetic rats	1380:1415	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	8	40	theme	optimized	1269:1277	arg1	formulation					1279:1289	The optimized formulation	1265:1289	The optimized formulation	1265:1289	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	1	41	theme	-bionanofiller	222:235	arg1	composites					237:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	7	42	theme	1:1	1135:1137	arg1	composites					1140:1149	DMP (1:1) composites	1130:1149	DMP (1:1) composites eluted MFM via case-II transport mechanism	1130:1192	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	4	43	theme	infrared	589:596	arg1	analyses					598:605	The thermal, X-ray and infrared analyses	566:605	analyses	598:605	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	8	44	theme	substantial	1345:1355	arg1	effect					1370:1375	substantial hypoglycemic effect	1345:1375	substantial hypoglycemic effect	1345:1375	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	8	45	theme	diabetic	1403:1410	arg1	rats					1412:1415	streptozotocin-induced diabetic rats	1380:1415	streptozotocin-induced diabetic rats	1380:1415	The optimized formulation demonstrated excellent gastroretentive properties and substantial hypoglycemic effect in streptozotocin-induced diabetic rats.					
29932998	1	46	theme	Olive	136:140	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	46	theme	Olive	136:140	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	6	47	dep	GG	852:853	arg1	DMP					855:857	DMP	855:857	DMP	855:857	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	9	48	theme	intragastric	1486:1497	arg1	delivery					1499:1506	controlled intragastric delivery	1475:1506	controlled intragastric delivery of MFM for the management of type 2 diabetes	1475:1551	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	0	49	theme	tailor-made	41:51	arg1	composites					85:94	tailor-made pectin-gellan gum-bionanofiller composites	41:94	tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery	41:124	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	7	50	theme	zero-order	1239:1248	arg1	model					1258:1262	zero-order kinetic model	1239:1262	zero-order kinetic model	1239:1262	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	5	51	theme	Scanning	738:745	arg1	microscopy					756:765	Scanning electron microscopy	738:765	Scanning electron microscopy	738:765	Scanning electron microscopy revealed almost spherical shape of the composites.					
29932998	7	52	theme	kinetic	1250:1256	arg1	model					1258:1262	zero-order kinetic model	1239:1262	zero-order kinetic model	1239:1262	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	4	53	with	compatible	650:659	arg1	drug					670:673	the drug	666:673	the drug	666:673	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	2	54	theme	DSC	410:412	arg1	analyses					414:421	DSC analyses	410:421	DSC analyses	410:421	DMP had a degree of amidation of 48.7% and was characterized further by FTIR, XRD and DSC analyses.					
29932998	4	55	theme	certain	683:689	arg1	degree					691:696	certain degree	683:696	certain degree of attenuation in drug's crystallinity	683:735	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	2	56	contain	had	328:330	arg1	DMP					324:326	DMP	324:326	DMP	324:326	DMP had a degree of amidation of 48.7% and was characterized further by FTIR, XRD and DSC analyses.					
29932998	2	56	contain	had	328:330	arg2	degree					334:339	a degree	332:339	a degree of amidation of 48.7%	332:361	DMP had a degree of amidation of 48.7% and was characterized further by FTIR, XRD and DSC analyses.					
29932998	5	57	theme	electron	747:754	arg1	microscopy					756:765	Scanning electron microscopy	738:765	Scanning electron microscopy	738:765	Scanning electron microscopy revealed almost spherical shape of the composites.					
29932998	6	58	theme	nanofiller	869:878	arg1	types					860:864	types	860:864	types of nanofiller (neusilin/bentonite/Florite) and oil inclusion	860:925	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	6	58	theme	nanofiller	869:878	arg1	GG					852:853	GG	852:853	GG	852:853	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	4	59	theme	compatible	650:659	arg1	composites					639:648	the composites	635:648	the composites compatible with the drug	635:673	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	9	60	theme	novel	1424:1428	arg1	matrices					1437:1444	These novel hybrid matrices	1418:1444	These novel hybrid matrices	1418:1444	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	0	61	theme	gum-bionanofiller	67:83	arg1	composites					85:94	tailor-made pectin-gellan gum-bionanofiller composites	41:94	tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery	41:124	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	6	62	theme	encapsulation	967:979	arg1	efficiency					981:990	variable drug encapsulation efficiency	953:990	variable drug encapsulation efficiency (DEE, 50-85%)	953:1004	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	9	63	theme	diabetes	1544:1551	arg1	management					1523:1532	the management	1519:1532	the management of type 2 diabetes	1519:1551	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	1	64	theme	oil-entrapped	142:154	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	64	theme	oil-entrapped	142:154	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	0	65	theme	pectin-gellan	53:65	arg1	composites					85:94	tailor-made pectin-gellan gum-bionanofiller composites	41:94	tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery	41:124	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	4	66	theme	thermal	570:576	arg1	X-ray					579:583	The thermal, X-ray and infrared analyses	566:605	X-ray	579:583	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	3	67	theme	zinc	536:539	arg1	acetate					541:547	zinc acetate	536:547	zinc acetate	536:547	MFM-loaded composites were subsequently accomplished by green synthesis via ionotropic gelation technique using zinc acetate as cross-linker.					
29932998	1	68	theme	diethanolamine-modified	156:178	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	68	theme	diethanolamine-modified	156:178	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	6	69	theme	variable	953:960	arg1	efficiency					981:990	variable drug encapsulation efficiency	953:990	variable drug encapsulation efficiency (DEE, 50-85%)	953:1004	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	7	70	theme	DMP	1130:1132	arg1	composites					1140:1149	DMP (1:1) composites	1130:1149	DMP (1:1) composites eluted MFM via case-II transport mechanism	1130:1192	The optimized oil-entrapped Florite R NF/GG: DMP (1:1) composites eluted MFM via case-II transport mechanism and its drug release data was best fitted in zero-order kinetic model.					
29932998	4	71	theme	attenuation	701:711	arg1	degree					691:696	certain degree	683:696	certain degree of attenuation in drug's crystallinity	683:735	The thermal, X-ray and infrared analyses suggested an environment in the composites compatible with the drug, except certain degree of attenuation in drug's crystallinity.					
29932998	1	72	theme	high-methoxyl	180:192	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	72	theme	high-methoxyl	180:192	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	9	73	theme	hybrid	1430:1435	arg1	matrices					1437:1444	These novel hybrid matrices	1418:1444	These novel hybrid matrices	1418:1444	These novel hybrid matrices were thus found suitable for controlled intragastric delivery of MFM for the management of type 2 diabetes.					
29932998	1	74	theme	pectin	194:199	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	74	theme	pectin	194:199	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	6	75	dep	efficiency	981:990	arg1	DEE					993:995	DEE	993:995	DEE	993:995	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	6	75	dep	efficiency	981:990	arg1	%					1003:1003	50-85%	998:1003	50-85%	998:1003	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29932998	1	76	theme	controlled	267:276	arg1	delivery					291:298	controlled intragastric delivery	267:298	controlled intragastric delivery of metformin HCl (MFM)	267:321	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	3	77	theme	green	480:484	arg1	synthesis					486:494	green synthesis	480:494	green synthesis	480:494	MFM-loaded composites were subsequently accomplished by green synthesis via ionotropic gelation technique using zinc acetate as cross-linker.					
29932998	3	78	theme	ionotropic	500:509	arg1	technique					520:528	ionotropic gelation technique	500:528	ionotropic gelation technique using zinc acetate as cross-linker	500:563	MFM-loaded composites were subsequently accomplished by green synthesis via ionotropic gelation technique using zinc acetate as cross-linker.					
29932998	1	79	theme	intragastric	278:289	arg1	delivery					291:298	controlled intragastric delivery	267:298	controlled intragastric delivery of metformin HCl (MFM)	267:321	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	0	80	theme	composites	85:94	arg1	characterization					21:36	characterization	21:36	characterization	21:36	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	0	80	theme	composites	85:94	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis and characterization of tailor-made pectin-gellan gum-bionanofiller composites as intragastric drug delivery shuttles.					
29932998	1	81	theme	DMP	202:204	arg1	GG					219:220	GG	219:220	GG	219:220	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	1	81	theme	DMP	202:204	arg1	gum					214:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum	136:216	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites	136:246	Olive oil-entrapped diethanolamine-modified high-methoxyl pectin (DMP)-gellan gum (GG)-bionanofiller composites were developed for controlled intragastric delivery of metformin HCl (MFM).					
29932998	6	82	theme	drug	1019:1022	arg1	behaviours					1032:1041	extended drug release behaviours	1010:1041	extended drug release behaviours (Q8h, 69-94%)	1010:1055	Depending upon the mass ratios of GG:DMP, types of nanofiller (neusilin/bentonite/Florite) and oil inclusion, the composites exhibited variable drug encapsulation efficiency (DEE, 50-85%) and extended drug release behaviours (Q8h, 69-94%) in acetate buffer (pH 4.5).					
29678921	9	0	theme	initiation	1596:1605	arg1	process					1607:1613	this growth initiation process	1584:1613	this growth initiation process	1584:1613	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	7	1	theme	environmental	1208:1220	arg1	bacteria					1222:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	11	2	theme	small	1970:1974	arg1	signal					1958:1963	the signal	1954:1963	the signal	1954:1963	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	11	2	theme	small	1970:1974	arg1	motif					1999:2003	a small repeating disaccharide motif	1968:2003	a small repeating disaccharide motif	1968:2003	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	5	3	theme	resuscitation	611:623	arg1	signal					625:630	resuscitation signal	611:630	resuscitation signal	611:630	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	4	4	theme	bacterial	549:557	arg1	species					559:565	bacterial species	549:565	bacterial species	549:565	Only a few resuscitation signals have been described to date, and all of them are produced endogenously by bacterial species.					
29678921	12	5	theme	growth	2107:2112	arg1	media					2114:2118	solid growth media	2101:2118	solid growth media	2101:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	6	6	theme	agarose	760:766	arg1	Fractionation					694:706	Fractionation	694:706	Fractionation of the agar polysaccharide complex	694:741	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	6	theme	agarose	760:766	arg1	sulfation					747:755	sulfation	747:755	sulfation of agarose	747:766	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	2	7	theme	dormant	230:236	arg1	state					238:242	a metabolically inactive dormant state	205:242	a metabolically inactive dormant state	205:242	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	9	8	theme	Micrococcus	1524:1534	arg1	luteus					1536:1541	Micrococcus luteus	1524:1541	Micrococcus luteus	1524:1541	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	5	9	theme	agarose	678:684	arg1	plates					686:691	agar but not agarose plates	665:691	plates	686:691	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	11	10	theme	disaccharide	1986:1997	arg1	signal					1958:1963	the signal	1954:1963	the signal	1954:1963	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	11	10	theme	disaccharide	1986:1997	arg1	motif					1999:2003	a small repeating disaccharide motif	1968:2003	a small repeating disaccharide motif	1968:2003	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	9	11	theme	apparent	1659:1666	arg1	connection					1668:1677	its apparent connection	1655:1677	its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen	1655:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	12	12	theme	polysaccharide	2071:2084	arg1	composition					2086:2096	the polysaccharide composition	2067:2096	the polysaccharide composition of solid growth media	2067:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	7	13	theme	polysaccharide	994:1007	arg1	fragment					967:974	a small fragment	959:974	a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated	959:1334	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	8	14	theme	phenomenon	1402:1411	arg1	manifestations					1379:1392	and manifestations	1375:1392	manifestations	1379:1392	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	12	15	theme	inherent	2040:2047	arg1	signals					2032:2038	signals	2032:2038	signals inherent in or absent from the polysaccharide composition of solid growth media	2032:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	7	16	link	1,4-linked	1133:1142	arg1	bacteria					1222:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	7	17	theme	large	981:985	arg1	polysaccharide					994:1007	a large linear polysaccharide	979:1007	a large linear polysaccharide	979:1007	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	9	18	theme	persistent	1688:1697	arg1	form					1710:1713	the persistent or dormant form	1684:1713	the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen	1684:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	2	19	theme	trigger	274:280	arg1	factors					282:288	specific chemical trigger factors	256:288	specific chemical trigger factors	256:288	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	9	20	theme	dormant	1702:1708	arg1	form					1710:1713	the persistent or dormant form	1684:1713	the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen	1684:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	0	21	theme	luteus	69:74	arg1	Bacteria					76:83	Dormant Micrococcus luteus Bacteria	49:83	Dormant Micrococcus luteus Bacteria	49:83	A Repeating Sulfated Galactan Motif Resuscitates Dormant Micrococcus luteus Bacteria.					
29678921	2	22	theme	specific	256:263	arg1	factors					282:288	specific chemical trigger factors	256:288	specific chemical trigger factors	256:288	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	7	23	theme	small	961:965	arg1	fragment					967:974	a small fragment	959:974	a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated	959:1334	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	6	24	located	found	833:837	arg2	signal					795:800	the signal	791:800	the signal	791:800	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	24	located	found	833:837	arg2	saccharides					821:831	highly sulfated saccharides	805:831	highly sulfated saccharides found in agar or carrageenans	805:861	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	24	located	found	833:837	arg1	carrageenans					850:861	carrageenans	850:861	carrageenans	850:861	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	24	located	found	833:837	arg1	agar					842:845	agar	842:845	agar	842:845	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	9	25	theme	tuberculosis	1732:1743	arg1	form					1710:1713	the persistent or dormant form	1684:1713	the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen	1684:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	7	26	contain	containing	1094:1103	arg1	motif					1088:1092	a repeating disaccharide motif	1063:1092	a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1063:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	7	26	contain	containing	1094:1103	arg2	bacteria					1222:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	5	27	theme	novel	597:601	arg1	type					603:606	a novel type	595:606	a novel type of resuscitation signal	595:630	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	7	28	theme	same	1299:1302	arg1	plates					1304:1309	the same plates	1295:1309	the same plates	1295:1309	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	12	29	contain	have	2124:2127	arg2	effects					2135:2141	major effects	2129:2141	major effects	2129:2141	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	12	29	contain	have	2124:2127	arg1	signals					2032:2038	signals	2032:2038	signals inherent in or absent from the polysaccharide composition of solid growth media	2032:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	8	30	theme	molecular	1471:1479	arg1	signal					1481:1486	a molecular signal	1469:1486	a molecular signal indicating safe growing conditions	1469:1521	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	1	31	theme	small	93:97	arg1	fraction					99:106	Only a small fraction	86:106	Only a small fraction of bacteria	86:118	Only a small fraction of bacteria can autonomously initiate growth on agar plates.					
29678921	7	32	with	oligosaccharide	1019:1033	arg1	motif					1088:1092	a repeating disaccharide motif	1063:1092	a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1063:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	3	33	theme	model	374:378	arg1	organism					380:387	a model organism	372:387	a model organism for this important yet poorly understood phenomenon	372:439	Micrococcus luteus has become a model organism for this important yet poorly understood phenomenon.					
29678921	0	34	theme	Sulfated	12:19	arg1	Galactan					21:28	A Repeating Sulfated Galactan	0:28	A Repeating Sulfated Galactan Motif	0:34	A Repeating Sulfated Galactan Motif Resuscitates Dormant Micrococcus luteus Bacteria.					
29678921	7	35	dep	initiate	1238:1245	arg1	i.e.					1010:1013	i.e.	1010:1013	i.e.	1010:1013	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	8	36	theme	growing	1504:1510	arg1	conditions					1512:1521	safe growing conditions	1499:1521	safe growing conditions	1499:1521	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	7	37	theme	1,4-linked	1133:1142	arg1	bacteria					1222:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	12	38	from	composition	2086:2096	arg1	absent					2055:2060	absent	2055:2060	absent	2055:2060	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	3	39	theme	important	398:406	arg1	phenomenon					430:439	this important yet poorly understood phenomenon	393:439	this important yet poorly understood phenomenon	393:439	Micrococcus luteus has become a model organism for this important yet poorly understood phenomenon.					
29678921	7	40	theme	Most	1203:1206	arg1	bacteria					1222:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1105:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	0	41	theme	Dormant	49:55	arg1	Bacteria					76:83	Dormant Micrococcus luteus Bacteria	49:83	Dormant Micrococcus luteus Bacteria	49:83	A Repeating Sulfated Galactan Motif Resuscitates Dormant Micrococcus luteus Bacteria.					
29678921	7	42	theme	repeating	1065:1073	arg1	motif					1088:1092	a repeating disaccharide motif	1063:1092	a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1063:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	6	43	theme	polysaccharide	720:733	arg1	complex					735:741	the agar polysaccharide complex	711:741	the agar polysaccharide complex	711:741	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	12	44	theme	major	2129:2133	arg1	effects					2135:2141	major effects	2129:2141	major effects	2129:2141	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	7	45	theme	κ-carrageenan	891:903	arg1	Purification					864:875	Purification	864:875	Purification of hydrolyzed κ-carrageenan	864:903	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	9	46	theme	growth	1589:1594	arg1	process					1607:1613	this growth initiation process	1584:1613	this growth initiation process	1584:1613	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	5	47	theme	signal	625:630	arg1	type					603:606	a novel type	595:606	a novel type of resuscitation signal	595:630	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	7	48	theme	disaccharide	1075:1086	arg1	motif					1088:1092	a repeating disaccharide motif	1063:1092	a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1063:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	10	49	from	agar	1837:1840	arg1	saccharide					1821:1830	a highly sulfated saccharide	1803:1830	a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates	1803:1919	In this report, we identify a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates.					
29678921	7	50	from	growth	1247:1252	arg1	plates					1262:1267	agar plates	1257:1267	agar plates	1257:1267	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	7	51	theme	sugars	1051:1056	arg1	oligosaccharide					1019:1033	an oligosaccharide	1016:1033	an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria	1016:1229	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	10	52	from	carrageenans	1845:1856	arg1	saccharide					1821:1830	a highly sulfated saccharide	1803:1830	a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates	1803:1919	In this report, we identify a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates.					
29678921	12	53	theme	solid	2101:2105	arg1	media					2114:2118	solid growth media	2101:2118	solid growth media	2101:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	5	54	theme	not	674:676	arg1	plates					686:691	agar but not agarose plates	665:691	plates	686:691	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	11	55	theme	repeating	1976:1984	arg1	signal					1958:1963	the signal	1954:1963	the signal	1954:1963	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	11	55	theme	repeating	1976:1984	arg1	motif					1999:2003	a small repeating disaccharide motif	1968:2003	a small repeating disaccharide motif	1968:2003	We identified and characterized the signal as a small repeating disaccharide motif.					
29678921	4	56	theme	resuscitation	453:465	arg1	signals					467:473	Only a few resuscitation signals	442:473	Only a few resuscitation signals	442:473	Only a few resuscitation signals have been described to date, and all of them are produced endogenously by bacterial species.					
29678921	10	57	theme	agarose	1906:1912	arg1	plates					1914:1919	agarose plates	1906:1919	agarose plates	1906:1919	In this report, we identify a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates.					
29678921	1	58	theme	bacteria	111:118	arg1	fraction					99:106	Only a small fraction	86:106	Only a small fraction of bacteria	86:118	Only a small fraction of bacteria can autonomously initiate growth on agar plates.					
29678921	8	59	theme	underlying	1418:1427	arg1	requirement					1453:1463	the requirement	1449:1463	the requirement for a molecular signal indicating safe growing conditions	1449:1521	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	8	59	theme	underlying	1418:1427	arg1	cause					1429:1433	the underlying cause	1414:1433	the underlying cause	1414:1433	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	5	60	theme	agar	665:668	arg1	plates					686:691	agar but not agarose plates	665:691	plates	686:691	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	7	61	theme	linear	987:992	arg1	polysaccharide					994:1007	a large linear polysaccharide	979:1007	a large linear polysaccharide	979:1007	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	2	62	theme	chemical	265:272	arg1	factors					282:288	specific chemical trigger factors	256:288	specific chemical trigger factors	256:288	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	3	63	theme	Micrococcus	342:352	arg1	luteus					354:359	Micrococcus luteus	342:359	Micrococcus luteus	342:359	Micrococcus luteus has become a model organism for this important yet poorly understood phenomenon.					
29678921	7	64	theme	DA-G4S	1184:1189	arg1	3,6-anhydro-α-d-galactose					1147:1171	3,6-anhydro-α-d-galactose	1147:1171	3,6-anhydro-α-d-galactose (DA)	1147:1176	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	7	64	theme	DA-G4S	1184:1189	arg1	IMPORTANCE					1192:1201	G4S-(DA-G4S) IMPORTANCE	1179:1201	G4S-(DA-G4S) IMPORTANCE	1179:1201	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	1	65	theme	agar	156:159	arg1	plates					161:166	agar plates	156:166	agar plates	156:166	Only a small fraction of bacteria can autonomously initiate growth on agar plates.					
29678921	9	66	theme	Mycobacterium	1718:1730	arg1	pathogen					1765:1772	an important human pathogen	1746:1772	an important human pathogen	1746:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	9	66	theme	Mycobacterium	1718:1730	arg1	tuberculosis					1732:1743	Mycobacterium tuberculosis	1718:1743	Mycobacterium tuberculosis	1718:1743	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	7	67	theme	G4S-	1179:1182	arg1	3,6-anhydro-α-d-galactose					1147:1171	3,6-anhydro-α-d-galactose	1147:1171	3,6-anhydro-α-d-galactose (DA)	1147:1176	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	7	67	theme	G4S-	1179:1182	arg1	IMPORTANCE					1192:1201	G4S-(DA-G4S) IMPORTANCE	1179:1201	G4S-(DA-G4S) IMPORTANCE	1179:1201	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	12	68	theme	media	2114:2118	arg1	composition					2086:2096	the polysaccharide composition	2067:2096	the polysaccharide composition of solid growth media	2067:2118	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	1	69	from	growth	146:151	arg1	plates					161:166	agar plates	156:166	agar plates	156:166	Only a small fraction of bacteria can autonomously initiate growth on agar plates.					
29678921	5	70	theme	type	603:606	arg1	discovery					582:590	the discovery	578:590	the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates	578:691	We report the discovery of a novel type of resuscitation signal that allows M. luteus to grow on agar but not agarose plates.					
29678921	9	71	theme	important	1749:1757	arg1	pathogen					1765:1772	an important human pathogen	1746:1772	an important human pathogen	1746:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	9	71	theme	important	1749:1757	arg1	tuberculosis					1732:1743	Mycobacterium tuberculosis	1718:1743	Mycobacterium tuberculosis	1718:1743	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	0	72	theme	Repeating	2:10	arg1	Galactan					21:28	A Repeating Sulfated Galactan	0:28	A Repeating Sulfated Galactan Motif	0:34	A Repeating Sulfated Galactan Motif Resuscitates Dormant Micrococcus luteus Bacteria.					
29678921	8	73	theme	safe	1499:1502	arg1	conditions					1512:1521	safe growing conditions	1499:1521	safe growing conditions	1499:1521	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	0	74	theme	Galactan	21:28	arg1	Motif					30:34	A Repeating Sulfated Galactan Motif	0:34	A Repeating Sulfated Galactan Motif	0:34	A Repeating Sulfated Galactan Motif Resuscitates Dormant Micrococcus luteus Bacteria.					
29678921	7	75	theme	signal	949:954	arg1	identification					927:940	the identification	923:940	the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated	923:1334	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	10	76	theme	sulfated	1812:1819	arg1	saccharide					1821:1830	a highly sulfated saccharide	1803:1830	a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates	1803:1919	In this report, we identify a highly sulfated saccharide from agar or carrageenans that robustly resuscitates dormant M. luteus on agarose plates.					
29678921	9	77	with	connection	1668:1677	arg1	form					1710:1713	the persistent or dormant form	1684:1713	the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen	1684:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	2	78	dep	require	248:254	arg1	resume					327:332	resume	327:332	to resume growth	324:339	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	2	78	dep	require	248:254	arg1	exit					304:307	exit	304:307	to exit this state	301:318	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	3	79	theme	understood	419:428	arg1	phenomenon					430:439	this important yet poorly understood phenomenon	393:439	this important yet poorly understood phenomenon	393:439	Micrococcus luteus has become a model organism for this important yet poorly understood phenomenon.					
29678921	2	80	theme	Nongrowing	169:178	arg1	bacteria					180:187	Nongrowing bacteria	169:187	Nongrowing bacteria	169:187	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	9	81	theme	model	1556:1560	arg1	organism					1562:1569	a model organism	1554:1569	a model organism for studying this growth initiation process, often called resuscitation	1554:1641	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	7	82	theme	agar	1257:1260	arg1	plates					1262:1267	agar plates	1257:1267	agar plates	1257:1267	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	2	83	theme	inactive	221:228	arg1	state					238:242	a metabolically inactive dormant state	205:242	a metabolically inactive dormant state	205:242	Nongrowing bacteria typically enter a metabolically inactive dormant state and require specific chemical trigger factors or signals to exit this state and to resume growth.					
29678921	12	84	theme	bacterial	2146:2154	arg1	growth					2156:2161	bacterial growth	2146:2161	bacterial growth	2146:2161	Our results indicate that signals inherent in or absent from the polysaccharide composition of solid growth media can have major effects on bacterial growth.					
29678921	6	85	theme	complex	735:741	arg1	Fractionation					694:706	Fractionation	694:706	Fractionation of the agar polysaccharide complex	694:741	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	85	theme	complex	735:741	arg1	sulfation					747:755	sulfation	747:755	sulfation of agarose	747:766	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	86	theme	sulfated	812:819	arg1	saccharides					821:831	highly sulfated saccharides	805:831	highly sulfated saccharides found in agar or carrageenans	805:861	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	6	86	theme	sulfated	812:819	arg1	signal					795:800	the signal	791:800	the signal	791:800	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	8	87	theme	names	1365:1369	arg1	number					1355:1360	a number	1353:1360	a number of names	1353:1369	While there are a number of names for and manifestations of this phenomenon, the underlying cause appears to be the requirement for a molecular signal indicating safe growing conditions.					
29678921	6	88	theme	agar	715:718	arg1	complex					735:741	the agar polysaccharide complex	711:741	the agar polysaccharide complex	711:741	Fractionation of the agar polysaccharide complex and sulfation of agarose allowed us to identify the signal as highly sulfated saccharides found in agar or carrageenans.					
29678921	7	89	theme	hydrolyzed	880:889	arg1	κ-carrageenan					891:903	hydrolyzed κ-carrageenan	880:903	hydrolyzed κ-carrageenan	880:903	Purification of hydrolyzed κ-carrageenan ultimately led to the identification of the signal as a small fragment of a large linear polysaccharide, i.e., an oligosaccharide of five or more sugars with a repeating disaccharide motif containing d-galactose-4-sulfate (G4S) 1,4-linked to 3,6-anhydro-α-d-galactose (DA), G4S-(DA-G4S) IMPORTANCE Most environmental bacteria cannot initiate growth on agar plates, but they can flourish on the same plates once growth is initiated.					
29678921	9	90	theme	human	1759:1763	arg1	pathogen					1765:1772	an important human pathogen	1746:1772	an important human pathogen	1746:1772	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
29678921	9	90	theme	human	1759:1763	arg1	tuberculosis					1732:1743	Mycobacterium tuberculosis	1718:1743	Mycobacterium tuberculosis	1718:1743	Micrococcus luteus has become a model organism for studying this growth initiation process, often called resuscitation, because of its apparent connection with the persistent or dormant form of Mycobacterium tuberculosis, an important human pathogen.					
30452992	8	0	theme	partial	1250:1256	arg1	activity					1268:1275	partial microbial activity	1250:1275	partial microbial activity against Staphylococcus aureus	1250:1305	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	1	theme	UV	1388:1389	arg1	materials					1411:1419	UV shielding packaging materials	1388:1419	UV shielding packaging materials	1388:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	4	2	theme	k-C/X/G	659:665	arg1	structure					612:620	structure	612:620	structure	612:620	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	2	theme	k-C/X/G	659:665	arg1	morphology					600:609	morphology	600:609	morphology	600:609	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	2	theme	k-C/X/G	659:665	arg1	microstructure					626:639	microstructure	626:639	microstructure	626:639	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	0	3	theme	nanocomposite	60:72	arg1	films					74:78	highly elastic, stretchable UV protective nanocomposite films	18:78	highly elastic, stretchable UV protective nanocomposite films	18:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	0	4	theme	gum	151:153	arg1	combination					98:108	a combination	96:108	a combination of k-Carrageenan, xanthan gum and gellan gum	96:153	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	6	5	theme	moisture	1014:1021	arg1	content					1023:1029	moisture content	1014:1029	moisture content of the films	1014:1042	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	6	6	theme	hydrophobic	923:933	arg1	nature					935:940	the hydrophobic nature	919:940	the hydrophobic nature of the TiO2 nanoparticles	919:966	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	1	7	theme	solvent	267:273	arg1	method					283:288	solvent casting method	267:288	solvent casting method	267:288	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	8	8	theme	food	1356:1359	arg1	industries					1374:1383	food and non-food industries	1356:1383	food and non-food industries as UV shielding packaging materials	1356:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	4	9	theme	XRD	725:727	arg1	analysis					737:744	FT-IR, XRD and SEM analysis	718:744	FT-IR, XRD and SEM analysis	718:744	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	1	10	theme	casting	275:281	arg1	method					283:288	solvent casting method	267:288	solvent casting method	267:288	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	8	11	theme	microbial	1258:1266	arg1	activity					1268:1275	partial microbial activity	1250:1275	partial microbial activity against Staphylococcus aureus	1250:1305	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	12	from	application	1341:1351	arg1	industries					1374:1383	food and non-food industries	1356:1383	food and non-food industries as UV shielding packaging materials	1356:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	1	13	theme	xanthan	229:235	arg1	X					242:242	X	242:242	X	242:242	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	1	13	theme	xanthan	229:235	arg1	gum					237:239	xanthan gum	229:239	xanthan gum (X)	229:243	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	7	14	theme	violet	1100:1105	arg1	transmittance					1114:1126	ultra violet lights transmittance	1094:1126	ultra violet lights transmittance	1094:1126	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	5	15	theme	k-C/X/G	783:789	arg1	films					805:809	the k-C/X/G nanocomposite films	779:809	the k-C/X/G nanocomposite films	779:809	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	2	16	contain	have	386:389	arg2	elasticity					410:419	good elasticity	405:419	good elasticity	405:419	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	2	16	contain	have	386:389	arg2	texture					396:402	soft texture	391:402	soft texture	391:402	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	2	16	contain	have	386:389	arg1	films					307:311	The transparent films	291:311	The transparent films made from these gellable materials with synergistic hydrogel composition	291:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	2	16	contain	have	386:389	arg2	strength					441:448	excellent break strength	425:448	excellent break strength	425:448	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	3	17	theme	based	463:467	arg1	films					483:487	The k-C/X/G based nanocomposite films	451:487	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles	451:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	6	18	theme	contact	992:998	arg1	angle					1000:1004	contact angle	992:1004	contact angle	992:1004	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	2	19	theme	soft	391:394	arg1	texture					396:402	soft texture	391:402	soft texture	391:402	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	5	20	from	incorporation	754:766	arg1	films					805:809	the k-C/X/G nanocomposite films	779:809	the k-C/X/G nanocomposite films	779:809	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	2	21	theme	good	405:408	arg1	elasticity					410:419	good elasticity	405:419	good elasticity	405:419	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	3	22	theme	nanocomposite	469:481	arg1	films					483:487	The k-C/X/G based nanocomposite films	451:487	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles	451:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	2	23	with	materials	338:346	arg1	composition					374:384	synergistic hydrogel composition	353:384	synergistic hydrogel composition	353:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	0	24	theme	protective	49:58	arg1	films					74:78	highly elastic, stretchable UV protective nanocomposite films	18:78	highly elastic, stretchable UV protective nanocomposite films	18:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	3	25	theme	weight	513:518	arg1	ratio					520:524	different weight ratio	503:524	different weight ratio of TiO2 nanoparticles	503:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	5	26	theme	tensile	816:822	arg1	strength					824:831	the tensile strength	812:831	the tensile strength	812:831	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	7	27	theme	ultra	1094:1098	arg1	transmittance					1114:1126	ultra violet lights transmittance	1094:1126	ultra violet lights transmittance	1094:1126	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	6	28	from	increase	980:987	arg1	angle					1000:1004	contact angle	992:1004	contact angle	992:1004	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	1	29	theme	hydrogel	160:167	arg1	film					185:188	The hydrogel based composite film	156:188	The hydrogel based composite film	156:188	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	2	30	theme	hydrogel	365:372	arg1	composition					374:384	synergistic hydrogel composition	353:384	synergistic hydrogel composition	353:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	8	31	theme	non-food	1365:1372	arg1	industries					1374:1383	food and non-food industries	1356:1383	food and non-food industries as UV shielding packaging materials	1356:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	32	theme	UV	1230:1231	arg1	light					1233:1237	the UV light	1226:1237	the UV light	1226:1237	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	4	33	theme	FT-IR	718:722	arg1	analysis					737:744	FT-IR, XRD and SEM analysis	718:744	FT-IR, XRD and SEM analysis	718:744	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	34	dep	morphology	600:609	arg1	The					596:598	The	596:598	The	596:598	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	34	dep	morphology	600:609	arg1	properties					641:650	properties	641:650	properties	641:650	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	1	35	theme	based	169:173	arg1	film					185:188	The hydrogel based composite film	156:188	The hydrogel based composite film	156:188	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	0	36	theme	TiO2	10:13	arg1	Effect					0:5	Effect	0:5	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films	0:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	4	37	theme	SEM	733:735	arg1	analysis					737:744	FT-IR, XRD and SEM analysis	718:744	FT-IR, XRD and SEM analysis	718:744	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	3	38	theme	different	503:511	arg1	ratio					520:524	different weight ratio	503:524	different weight ratio of TiO2 nanoparticles	503:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	1	39	theme	composite	175:183	arg1	film					185:188	The hydrogel based composite film	156:188	The hydrogel based composite film	156:188	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	0	40	theme	elastic	25:31	arg1	films					74:78	highly elastic, stretchable UV protective nanocomposite films	18:78	highly elastic, stretchable UV protective nanocomposite films	18:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	5	41	theme	nanocomposite	791:803	arg1	films					805:809	the k-C/X/G nanocomposite films	779:809	the k-C/X/G nanocomposite films	779:809	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	3	42	theme	nanoparticles	534:546	arg1	ratio					520:524	different weight ratio	503:524	different weight ratio of TiO2 nanoparticles	503:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	2	43	theme	transparent	295:305	arg1	films					307:311	The transparent films	291:311	The transparent films made from these gellable materials with synergistic hydrogel composition	291:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	2	44	theme	gellable	329:336	arg1	materials					338:346	these gellable materials	323:346	these gellable materials with synergistic hydrogel composition	323:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	0	45	theme	k-Carrageenan	113:125	arg1	combination					98:108	a combination	96:108	a combination of k-Carrageenan, xanthan gum and gellan gum	96:153	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	7	46	theme	vapor	1065:1069	arg1	permeability					1071:1082	The water vapor permeability	1055:1082	The water vapor permeability (WVP)	1055:1088	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	7	46	theme	vapor	1065:1069	arg1	WVP					1085:1087	WVP	1085:1087	WVP	1085:1087	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	5	47	theme	TiO2	771:774	arg1	incorporation					754:766	the incorporation	750:766	the incorporation of TiO2 in the k-C/X/G nanocomposite films	750:809	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	7	48	theme	lights	1107:1112	arg1	transmittance					1114:1126	ultra violet lights transmittance	1094:1126	ultra violet lights transmittance	1094:1126	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	7	49	theme	water	1059:1063	arg1	permeability					1071:1082	The water vapor permeability	1055:1082	The water vapor permeability (WVP)	1055:1088	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	7	49	theme	water	1059:1063	arg1	WVP					1085:1087	WVP	1085:1087	WVP	1085:1087	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	1	50	theme	gellan	249:254	arg1	G					261:261	G	261:261	G	261:261	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	1	50	theme	gellan	249:254	arg1	gum					256:258	gellan gum	249:258	gellan gum (G)	249:262	The hydrogel based composite film was prepared from k-Carrageenan (k-C), xanthan gum (X) and gellan gum (G) by solvent casting method.					
30452992	0	51	theme	gum	136:138	arg1	combination					98:108	a combination	96:108	a combination of k-Carrageenan, xanthan gum and gellan gum	96:153	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	2	52	theme	excellent	425:433	arg1	strength					441:448	excellent break strength	425:448	excellent break strength	425:448	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	0	53	dep	elastic	25:31	arg1	stretchable					34:44	stretchable	34:44	stretchable	34:44	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	4	54	theme	TiO2	671:674	arg1	films					690:694	the k-C/X/G and TiO2 nanocomposite films	655:694	films	690:694	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	0	55	from	Effect	0:5	arg1	films					74:78	highly elastic, stretchable UV protective nanocomposite films	18:78	highly elastic, stretchable UV protective nanocomposite films	18:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	0	56	theme	xanthan	128:134	arg1	gum					136:138	xanthan gum	128:138	xanthan gum	128:138	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	3	57	theme	TiO2	529:532	arg1	nanoparticles					534:546	TiO2 nanoparticles	529:546	TiO2 nanoparticles	529:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	8	58	theme	k-C/X/G	1175:1181	arg1	nanocomposites					1192:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	59	theme	shielding	1391:1399	arg1	materials					1411:1419	UV shielding packaging materials	1388:1419	UV shielding packaging materials	1388:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	0	60	theme	gellan	144:149	arg1	gum					151:153	gellan gum	144:153	gellan gum	144:153	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	8	61	theme	packaging	1401:1409	arg1	materials					1411:1419	UV shielding packaging materials	1388:1419	UV shielding packaging materials	1388:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	3	62	theme	new	571:573	arg1	materials					585:593	new packaging materials	571:593	new packaging materials	571:593	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	6	63	theme	TiO2	949:952	arg1	nanoparticles					954:966	the TiO2 nanoparticles	945:966	the TiO2 nanoparticles	945:966	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	4	64	theme	films	690:694	arg1	structure					612:620	structure	612:620	structure	612:620	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	64	theme	films	690:694	arg1	morphology					600:609	morphology	600:609	morphology	600:609	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	4	64	theme	films	690:694	arg1	microstructure					626:639	microstructure	626:639	microstructure	626:639	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	5	65	theme	films	883:887	arg1	modulus					842:848	tensile modulus	834:848	tensile modulus	834:848	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	5	65	theme	films	883:887	arg1	stability					866:874	thermal stability	858:874	thermal stability	858:874	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	5	65	theme	films	883:887	arg1	Tg					851:852	Tg	851:852	Tg	851:852	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	5	65	theme	films	883:887	arg1	strength					824:831	the tensile strength	812:831	the tensile strength	812:831	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	6	66	theme	nanoparticles	954:966	arg1	nature					935:940	the hydrophobic nature	919:940	the hydrophobic nature of the TiO2 nanoparticles	919:966	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	6	67	theme	films	1038:1042	arg1	content					1023:1029	moisture content	1014:1029	moisture content of the films	1014:1042	Due to the hydrophobic nature of the TiO2 nanoparticles there is an increase in contact angle whereas moisture content of the films decreased.					
30452992	3	68	theme	packaging	575:583	arg1	materials					585:593	new packaging materials	571:593	new packaging materials	571:593	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30452992	4	69	theme	nanocomposite	676:688	arg1	films					690:694	the k-C/X/G and TiO2 nanocomposite films	655:694	films	690:694	The morphology, structure and microstructure properties of the k-C/X/G and TiO2 nanocomposite films were characterized by FT-IR, XRD and SEM analysis.					
30452992	5	70	theme	thermal	858:864	arg1	stability					866:874	thermal stability	858:874	thermal stability	858:874	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	2	71	theme	break	435:439	arg1	strength					441:448	excellent break strength	425:448	excellent break strength	425:448	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	8	72	theme	high	1318:1321	arg1	potential					1323:1331	a high potential	1316:1331	a high potential for the application in food and non-food industries as UV shielding packaging materials	1316:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	73	contain	have	1311:1314	arg2	potential					1323:1331	a high potential	1316:1331	a high potential for the application in food and non-food industries as UV shielding packaging materials	1316:1419	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	73	contain	have	1311:1314	arg1	nanocomposites					1192:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	8	74	theme	TiO2	1187:1190	arg1	nanocomposites					1192:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites	1171:1205	The k-C/X/G and TiO2 nanocomposites effectively shield the UV light, exhibited partial microbial activity against Staphylococcus aureus and have a high potential for the application in food and non-food industries as UV shielding packaging materials.					
30452992	5	75	theme	tensile	834:840	arg1	modulus					842:848	tensile modulus	834:848	tensile modulus	834:848	By the incorporation of TiO2 in the k-C/X/G nanocomposite films, the tensile strength, tensile modulus, Tg and thermal stability of the films were greatly enhanced.					
30452992	7	76	theme	TiO2	1157:1160	arg1	content					1162:1168	the TiO2 content	1153:1168	the TiO2 content	1153:1168	The water vapor permeability (WVP) and ultra violet lights transmittance decrease upon increasing the TiO2 content.					
30452992	0	77	theme	UV	46:47	arg1	films					74:78	highly elastic, stretchable UV protective nanocomposite films	18:78	highly elastic, stretchable UV protective nanocomposite films	18:78	Effect of TiO2 on highly elastic, stretchable UV protective nanocomposite films formed by using a combination of k-Carrageenan, xanthan gum and gellan gum.					
30452992	2	78	theme	synergistic	353:363	arg1	composition					374:384	synergistic hydrogel composition	353:384	synergistic hydrogel composition	353:384	The transparent films made from these gellable materials with synergistic hydrogel composition have soft texture, good elasticity and excellent break strength.					
30452992	3	79	theme	k-C/X/G	455:461	arg1	films					483:487	The k-C/X/G based nanocomposite films	451:487	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles	451:546	The k-C/X/G based nanocomposite films prepared from different weight ratio of TiO2 nanoparticles were characterized for new packaging materials.					
30497824	4	0	theme	evaporative	894:904	arg1	scattering					912:921	evaporative light scattering	894:921	evaporative light scattering detector (ELSD)	894:937	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	1	used	used	380:383	arg2	mixtures					348:355	Racemic mixtures	340:355	Racemic mixtures of 14 amino acids	340:373	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	4	2	dep	particular	808:817	arg1	in					805:806	in	805:806	in	805:806	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	0	3	theme	fluid	106:110	arg1	chromatography					112:125	supercritical fluid chromatography	92:125	supercritical fluid chromatography	92:125	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	4	4	theme	scattering	912:921	arg1	ELSD					933:936	ELSD	933:936	ELSD	933:936	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	4	theme	scattering	912:921	arg1	detector					923:930	evaporative light scattering detector	894:930	evaporative light scattering detector (ELSD)	894:937	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	1	5	theme	sub/super-critical	293:310	arg1	SFC					334:336	SFC	334:336	SFC	334:336	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	5	theme	sub/super-critical	293:310	arg1	chromatography					318:331	sub/super-critical fluid chromatography	293:331	sub/super-critical fluid chromatography (SFC)	293:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	0	6	theme	supercritical	92:104	arg1	chromatography					112:125	supercritical fluid chromatography	92:125	supercritical fluid chromatography	92:125	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	4	7	theme	light	906:910	arg1	scattering					912:921	evaporative light scattering	894:921	evaporative light scattering detector (ELSD)	894:937	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	8	theme	mobile	779:784	arg1	composition					792:802	mobile phase composition	779:802	mobile phase composition (in particular, of the amount of additive used)	779:850	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	1	9	theme	fluid	312:316	arg1	SFC					334:336	SFC	334:336	SFC	334:336	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	9	theme	fluid	312:316	arg1	chromatography					318:331	sub/super-critical fluid chromatography	293:331	sub/super-critical fluid chromatography (SFC)	293:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	4	10	theme	contribution	878:889	arg1	effect					769:774	the effect	765:774	the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening	765:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	11	theme	composition	792:802	arg1	effect					769:774	the effect	765:774	the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening	765:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	12	theme	recognition	408:418	arg1	ability					420:426	the chiral recognition ability	397:426	the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	397:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	2	13	theme	coated	484:489	arg1	selectors					524:532	coated or covalently immobilized chiral selectors	484:532	coated or covalently immobilized chiral selectors	484:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	4	14	theme	potential	868:876	arg1	contribution					878:889	the potential contribution	864:889	the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening	864:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	15	theme	phase	786:790	arg1	composition					792:802	mobile phase composition	779:802	mobile phase composition (in particular, of the amount of additive used)	779:850	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	16	theme	chiral	401:406	arg1	ability					420:426	the chiral recognition ability	397:426	the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	397:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	4	17	theme	extra	942:946	arg1	band-broadening					955:969	extra column band-broadening	942:969	extra column band-broadening	942:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	18	theme	Racemic	340:346	arg1	mixtures					348:355	Racemic mixtures	340:355	Racemic mixtures of 14 amino acids	340:373	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	1	19	theme	enantiomers	210:220	arg1	separation					196:205	the separation	192:205	the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	192:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	0	20	theme	enantiomers	14:24	arg1	Separation					0:9	Separation	0:9	Separation of enantiomers of native amino acids with polysaccharide-based chiral	0:79	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	4	21	theme	column	948:953	arg1	band-broadening					955:969	extra column band-broadening	942:969	extra column band-broadening	942:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	22	theme	polysaccharide-based	443:462	arg1	columns					471:477	8 different polysaccharide-based chiral columns	431:477	8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	431:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	0	23	theme	amino	36:40	arg1	acids					42:46	native amino acids	29:46	native amino acids	29:46	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	3	24	theme	studied	545:551	arg1	columns					553:559	the studied columns	541:559	the studied columns	541:559	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	1	25	from	columns	282:288	arg1	SFC					334:336	SFC	334:336	SFC	334:336	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	25	from	columns	282:288	arg1	chromatography					318:331	sub/super-critical fluid chromatography	293:331	sub/super-critical fluid chromatography (SFC)	293:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	25	from	columns	282:288	arg1	enantiomers					210:220	enantiomers	210:220	enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	210:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	2	26	theme	different	433:441	arg1	columns					471:477	8 different polysaccharide-based chiral columns	431:477	8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	431:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	3	27	theme	chiral	648:653	arg1	ability					667:673	the most universal chiral recognition ability	629:673	the most universal chiral recognition ability	629:673	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	1	28	theme	underivatized	225:237	arg1	acids					245:249	underivatized amino acids	225:249	underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	225:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	0	29	theme	native	29:34	arg1	acids					42:46	native amino acids	29:46	native amino acids	29:46	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	2	30	theme	chiral	517:522	arg1	selectors					524:532	coated or covalently immobilized chiral selectors	484:532	coated or covalently immobilized chiral selectors	484:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	3	31	theme	recognition	655:665	arg1	ability					667:673	the most universal chiral recognition ability	629:673	the most universal chiral recognition ability	629:673	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	1	32	theme	amino	239:243	arg1	acids					245:249	underivatized amino acids	225:249	underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	225:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	2	33	theme	immobilized	505:515	arg1	selectors					524:532	coated or covalently immobilized chiral selectors	484:532	coated or covalently immobilized chiral selectors	484:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	2	34	with	columns	471:477	arg1	selectors					524:532	coated or covalently immobilized chiral selectors	484:532	coated or covalently immobilized chiral selectors	484:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	0	35	theme	acids	42:46	arg1	enantiomers					14:24	enantiomers	14:24	enantiomers of native amino acids	14:46	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	4	36	theme	detector	923:930	arg1	contribution					878:889	the potential contribution	864:889	the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening	864:969	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	36	theme	detector	923:930	arg1	composition					792:802	mobile phase composition	779:802	mobile phase composition (in particular, of the amount of additive used)	779:850	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	3	37	theme	amino	739:743	arg1	acids					745:749	10 studied amino acids	728:749	10 studied amino acids	728:749	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	3	38	theme	universal	638:646	arg1	ability					667:673	the most universal chiral recognition ability	629:673	the most universal chiral recognition ability	629:673	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	1	39	from	enantiomers	210:220	arg1	columns					282:288	polysaccharide-based chiral columns	254:288	polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	254:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	2	40	theme	acids	369:373	arg1	mixtures					348:355	Racemic mixtures	340:355	Racemic mixtures of 14 amino acids	340:373	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	4	41	theme	additive	837:844	arg1	additive					837:844	additive	837:844	additive	837:844	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	4	41	theme	additive	837:844	arg1	amount					827:832	the amount	823:832	the amount of additive used	823:849	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	1	42	from	acids	245:249	arg1	columns					282:288	polysaccharide-based chiral columns	254:288	polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	254:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	3	43	theme	acids	745:749	arg1	enantiomers					713:723	the enantiomers	709:723	the enantiomers of 10 studied amino acids	709:749	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	3	44	theme	baseline	685:692	arg1	resolution					694:703	baseline resolution	685:703	baseline resolution	685:703	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	0	45	with	Separation	0:9	arg1	chiral					74:79	chiral	74:79	chiral	74:79	Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.					
30497824	1	46	theme	short	136:140	arg1	communication					142:154	this short communication	131:154	this short communication	131:154	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	47	theme	acids	245:249	arg1	enantiomers					210:220	enantiomers	210:220	enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	210:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	4	48	theme	amount	827:832	arg1	particular					808:817	particular	808:817	particular	808:817	In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.					
30497824	2	49	theme	amino	363:367	arg1	acids					369:373	14 amino acids	360:373	14 amino acids	360:373	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	3	50	theme	studied	731:737	arg1	acids					745:749	10 studied amino acids	728:749	10 studied amino acids	728:749	Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids.					
30497824	2	51	theme	chiral	464:469	arg1	columns					471:477	8 different polysaccharide-based chiral columns	431:477	8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	431:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	1	52	theme	preliminary	156:166	arg1	results					168:174	preliminary results	156:174	preliminary results	156:174	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	1	53	theme	polysaccharide-based	254:273	arg1	columns					282:288	polysaccharide-based chiral columns	254:288	polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	254:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30497824	2	54	theme	columns	471:477	arg1	ability					420:426	the chiral recognition ability	397:426	the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors	397:532	Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors.					
30497824	1	55	theme	chiral	275:280	arg1	columns					282:288	polysaccharide-based chiral columns	254:288	polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC)	254:337	In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC).					
30639652	4	0	dep	Fourier	475:481	arg1	transform					483:491	transform	483:491	transform infrared (FTIR) spectroscopy	483:520	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	1	theme	electron	448:455	arg1	microscopy					457:466	scanning electron microscopy	439:466	scanning electron microscopy (SEM)	439:472	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	1	theme	electron	448:455	arg1	SEM					469:471	SEM	469:471	SEM	469:471	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	5	2	theme	random	674:679	arg1	orientation					681:691	random orientation	674:691	random orientation	674:691	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	2	3	dep	reinforcement	263:275	arg1	wt					280:281	1 wt	278:281	reinforcement (1 wt	263:281	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	2	4	used	used	255:258	arg2	TLMFs					243:247	TLMFs	243:247	TLMFs	243:247	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	2	4	used	used	255:258	arg2	reinforcement					263:275	reinforcement	263:275	reinforcement (1 wt	263:281	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	2	4	used	used	255:258	arg2	microfibers					230:240	The TL microfibers	223:240	The TL microfibers (TLMFs)	223:248	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	3	5	theme	5 wt	287:290	arg1	%					291:291	5 wt%)	287:292	5 wt%)	287:292	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	6	6	theme	chemical	817:824	arg1	treatment					826:834	chemical treatment	817:834	chemical treatment of fibers	817:844	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	8	7	theme	Thermal	937:943	arg1	stability					945:953	Thermal stability	937:953	Thermal stability of the composite films	937:976	Thermal stability of the composite films was slightly higher than the matrix.					
30639652	7	8	theme	higher	874:879	arg1	index					895:899	higher crystallinity index	874:899	higher crystallinity index for the composites than the matrix	874:934	The XRD analysis indicated higher crystallinity index for the composites than the matrix.					
30639652	6	9	theme	quantity	767:774	arg1	presence					749:756	the presence	745:756	the presence of minor quantity of lignin	745:784	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	5	10	theme	uniform	637:643	arg1	distribution					645:656	the uniform distribution	633:656	the uniform distribution of TLMFs but in random orientation in the composites	633:709	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	9	11	theme	TLMFs	1195:1199	arg1	orientation					1176:1186	random orientation	1169:1186	random orientation of the TLMFs in the composites	1169:1217	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	6	12	theme	spectral	717:724	arg1	analysis					726:733	FTIR spectral analysis	712:733	FTIR spectral analysis	712:733	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	5	13	theme	TLMFs	661:665	arg1	distribution					645:656	the uniform distribution	633:656	the uniform distribution of TLMFs but in random orientation in the composites	633:709	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	10	14	theme	tensile	1232:1238	arg1	properties					1240:1249	the tensile properties	1228:1249	the tensile properties	1228:1249	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	4	15	theme	composite	365:373	arg1	films					375:379	These composite films	359:379	These composite films	359:379	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	9	16	from	break	1087:1091	arg1	higher					1120:1125	higher	1120:1125	higher	1120:1125	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	9	16	from	break	1087:1091	arg1	elongation					1073:1082	the elongation	1069:1082	the elongation at break of the composite films	1069:1114	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	4	17	theme	X-ray	523:527	arg1	XRD					542:544	XRD	542:544	XRD	542:544	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	17	theme	X-ray	523:527	arg1	diffraction					529:539	X-ray diffraction	523:539	X-ray diffraction (XRD)	523:545	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	8	18	theme	films	972:976	arg1	stability					945:953	Thermal stability	937:953	Thermal stability of the composite films	937:976	Thermal stability of the composite films was slightly higher than the matrix.					
30639652	6	19	theme	FTIR	712:715	arg1	analysis					726:733	FTIR spectral analysis	712:733	FTIR spectral analysis	712:733	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	3	20	theme	cellulose	297:305	arg1	matrix					307:312	cellulose matrix	297:312	cellulose matrix to prepare cellulose /TLMFs composite films	297:356	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	4	21	theme	scanning	439:446	arg1	microscopy					457:466	scanning electron microscopy	439:466	scanning electron microscopy (SEM)	439:472	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	21	theme	scanning	439:446	arg1	SEM					469:471	SEM	469:471	SEM	469:471	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	8	22	theme	composite	962:970	arg1	films					972:976	the composite films	958:976	the composite films	958:976	Thermal stability of the composite films was slightly higher than the matrix.					
30639652	1	23	from	lampas	138:143	arg1	fibers					89:94	The fibers	85:94	The fibers from the newly identified plant Thespesia lampas (TL)	85:148	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	10	24	theme	TL	1374:1375	arg1	composites					1383:1392	the cellulose/alkali treated TL fiber composites	1345:1392	the cellulose/alkali treated TL fiber composites	1345:1392	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	3	25	theme	/TLMFs	335:340	arg1	films					352:356	cellulose /TLMFs composite films	325:356	cellulose /TLMFs composite films	325:356	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	4	26	dep	infrared	493:500	arg1	FTIR					503:506	FTIR	503:506	FTIR	503:506	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	10	27	theme	treated	1366:1372	arg1	composites					1383:1392	the cellulose/alkali treated TL fiber composites	1345:1392	the cellulose/alkali treated TL fiber composites	1345:1392	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	7	28	theme	XRD	851:853	arg1	analysis					855:862	The XRD analysis	847:862	The XRD analysis	847:862	The XRD analysis indicated higher crystallinity index for the composites than the matrix.					
30639652	3	29	theme	composite	342:350	arg1	films					352:356	cellulose /TLMFs composite films	325:356	cellulose /TLMFs composite films	325:356	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	10	30	theme	cellulose/alkali	1349:1364	arg1	composites					1383:1392	the cellulose/alkali treated TL fiber composites	1345:1392	the cellulose/alkali treated TL fiber composites	1345:1392	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	10	31	theme	fiber	1377:1381	arg1	composites					1383:1392	the cellulose/alkali treated TL fiber composites	1345:1392	the cellulose/alkali treated TL fiber composites	1345:1392	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	4	32	theme	tensile	585:591	arg1	tests					593:597	tensile tests	585:597	tensile tests	585:597	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	33	theme	infrared	493:500	arg1	spectroscopy					509:520	infrared (FTIR) spectroscopy	493:520	infrared (FTIR) spectroscopy	493:520	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	5	34	theme	SEM	612:614	arg1	images					616:621	The POM and SEM images	600:621	The POM and SEM images	600:621	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	9	35	theme	composite	1100:1108	arg1	films					1110:1114	the composite films	1096:1114	the composite films	1096:1114	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	9	36	theme	Tensile	1015:1021	arg1	modulus					1023:1029	Tensile modulus and tensile strength	1015:1050	modulus	1023:1029	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	3	37	theme	cellulose	325:333	arg1	films					352:356	cellulose /TLMFs composite films	325:356	cellulose /TLMFs composite films	325:356	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	4	38	theme	optical	413:419	arg1	POM					433:435	POM	433:435	POM	433:435	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	38	theme	optical	413:419	arg1	microscopy					421:430	polarized optical microscopy	403:430	polarized optical microscopy (POM)	403:436	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	1	39	theme	identified	111:120	arg1	lampas					138:143	the newly identified plant Thespesia lampas	101:143	the newly identified plant Thespesia lampas (TL)	101:148	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	1	39	theme	identified	111:120	arg1	TL					146:147	TL	146:147	TL	146:147	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	4	40	theme	thermogravimetric	548:564	arg1	TGA					576:578	TGA	576:578	TGA	576:578	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	40	theme	thermogravimetric	548:564	arg1	analysis					566:573	thermogravimetric analysis	548:573	thermogravimetric analysis (TGA)	548:579	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	0	41	theme	lampas	50:55	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of cellulose/Thespesia lampas microfiber composite films.					
30639652	0	41	theme	lampas	50:55	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose/Thespesia lampas microfiber composite films.					
30639652	4	42	theme	polarized	403:411	arg1	POM					433:435	POM	433:435	POM	433:435	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	4	42	theme	polarized	403:411	arg1	microscopy					421:430	polarized optical microscopy	403:430	polarized optical microscopy (POM)	403:436	These composite films were characterized by polarized optical microscopy (POM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and tensile tests.					
30639652	2	43	theme	TL	227:228	arg1	reinforcement					263:275	reinforcement	263:275	reinforcement (1 wt	263:281	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	2	43	theme	TL	227:228	arg1	TLMFs					243:247	TLMFs	243:247	TLMFs	243:247	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	2	43	theme	TL	227:228	arg1	microfibers					230:240	The TL microfibers	223:240	The TL microfibers (TLMFs)	223:248	The TL microfibers (TLMFs) were used as reinforcement (1 wt.					
30639652	0	44	theme	cellulose/Thespesia	30:48	arg1	lampas					50:55	cellulose/Thespesia lampas	30:55	cellulose/Thespesia lampas	30:55	Preparation and properties of cellulose/Thespesia lampas microfiber composite films.					
30639652	9	45	from	TLMFs	1195:1199	arg1	composites					1208:1217	the composites	1204:1217	the composites	1204:1217	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	9	46	theme	tensile	1035:1041	arg1	strength					1043:1050	Tensile modulus and tensile strength	1015:1050	strength	1043:1050	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	1	47	theme	aspect	209:214	arg1	ratio					216:220	their aspect ratio	203:220	their aspect ratio	203:220	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	0	48	theme	composite	68:76	arg1	films					78:82	composite films	68:82	composite films	68:82	Preparation and properties of cellulose/Thespesia lampas microfiber composite films.					
30639652	10	49	theme	packaging	1426:1434	arg1	applications					1436:1447	packaging applications	1426:1447	packaging applications	1426:1447	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	5	50	from	distribution	645:656	arg1	composites					700:709	the composites	696:709	the composites	696:709	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	5	50	from	distribution	645:656	arg1	orientation					681:691	random orientation	674:691	random orientation	674:691	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	5	51	theme	POM	604:606	arg1	images					616:621	The POM and SEM images	600:621	The POM and SEM images	600:621	The POM and SEM images indicated the uniform distribution of TLMFs but in random orientation in the composites.					
30639652	1	52	theme	plant	122:126	arg1	lampas					138:143	the newly identified plant Thespesia lampas	101:143	the newly identified plant Thespesia lampas (TL)	101:148	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	1	52	theme	plant	122:126	arg1	TL					146:147	TL	146:147	TL	146:147	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	10	53	theme	composites	1296:1305	arg1	properties					1240:1249	the tensile properties	1228:1249	the tensile properties	1228:1249	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	10	53	theme	composites	1296:1305	arg1	stability					1263:1271	thermal stability	1255:1271	thermal stability	1255:1271	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	6	54	theme	minor	761:765	arg1	quantity					767:774	minor quantity	761:774	minor quantity	761:774	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	9	55	theme	cellulose	1145:1153	arg1	matrix					1155:1160	the cellulose matrix	1141:1160	the cellulose matrix	1141:1160	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	1	56	theme	Thespesia	128:136	arg1	lampas					138:143	the newly identified plant Thespesia lampas	101:143	the newly identified plant Thespesia lampas (TL)	101:148	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	1	56	theme	Thespesia	128:136	arg1	TL					146:147	TL	146:147	TL	146:147	The fibers from the newly identified plant Thespesia lampas (TL) were converted into microfibers in order to increase their aspect ratio.					
30639652	6	57	theme	fibers	839:844	arg1	treatment					826:834	chemical treatment	817:834	chemical treatment of fibers	817:844	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	3	58	dep	matrix	307:312	arg1	%					291:291	5 wt%)	287:292	5 wt%)	287:292	to 5 wt%) in cellulose matrix to prepare cellulose /TLMFs composite films.					
30639652	9	59	theme	films	1110:1114	arg1	higher					1120:1125	higher	1120:1125	higher	1120:1125	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	9	59	theme	films	1110:1114	arg1	elongation					1073:1082	the elongation	1069:1082	the elongation at break of the composite films	1069:1114	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	7	60	theme	crystallinity	881:893	arg1	index					895:899	higher crystallinity index	874:899	higher crystallinity index for the composites than the matrix	874:934	The XRD analysis indicated higher crystallinity index for the composites than the matrix.					
30639652	6	61	theme	lignin	779:784	arg1	presence					749:756	the presence	745:756	the presence of minor quantity of lignin	745:784	FTIR spectral analysis indicated the presence of minor quantity of lignin in the microfibers in spite of chemical treatment of fibers.					
30639652	9	62	theme	random	1169:1174	arg1	orientation					1176:1186	random orientation	1169:1186	random orientation of the TLMFs in the composites	1169:1217	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	10	63	theme	cellulose/TLMFs	1280:1294	arg1	composites					1296:1305	the cellulose/TLMFs composites	1276:1305	the cellulose/TLMFs composites	1276:1305	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	9	64	from	orientation	1176:1186	arg1	composites					1208:1217	the composites	1204:1217	the composites	1204:1217	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
30639652	10	65	theme	thermal	1255:1261	arg1	stability					1263:1271	thermal stability	1255:1271	thermal stability	1255:1271	However the tensile properties and thermal stability of the cellulose/TLMFs composites were found to be higher than those of the cellulose/alkali treated TL fiber composites and hence can be considered for packaging applications.					
30639652	9	66	from	composites	1208:1217	arg1	orientation					1176:1186	random orientation	1169:1186	random orientation of the TLMFs in the composites	1169:1217	Tensile modulus and tensile strength were lower while the elongation at break of the composite films was higher than those of the cellulose matrix due to random orientation of the TLMFs in the composites.					
31427006	0	0	theme	endothelial	114:124	arg1	proliferation					131:143	endothelial cell proliferation	114:143	endothelial cell proliferation	114:143	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	2	1	theme	oHAs	416:419	arg1	sizes					407:411	different sizes	397:411	different sizes of oHAs	397:419	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	4	2	from	hemocompatibility	796:812	arg1	scaffolds					860:868	these nanofibrous scaffolds	842:868	these nanofibrous scaffolds	842:868	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	1	3	theme	promising	197:205	arg1	results					207:213	promising results	197:213	promising results	197:213	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	5	4	theme	molecular	1048:1056	arg1	weight					1058:1063	high molecular weight	1043:1063	high molecular weight	1043:1063	The results showed that the nanofibrous scaffolds grafted by oHAs promoted EC proliferation whereas high molecular weight HA inhibited proliferation.					
31427006	4	5	gly	glycosylated	690:701	arg1	collagens					703:711	The glycosylated collagens	686:711	The glycosylated collagens	686:711	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	2	6	theme	EC	349:350	arg1	proliferation					352:364	EC proliferation	349:364	EC proliferation	349:364	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	0	7	theme	cell	126:129	arg1	proliferation					131:143	endothelial cell proliferation	114:143	endothelial cell proliferation	114:143	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	4	8	from	responses	829:837	arg1	scaffolds					860:868	these nanofibrous scaffolds	842:868	these nanofibrous scaffolds	842:868	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	6	9	theme	engineered	1190:1199	arg1	scaffolds					1217:1225	engineered vascular tissue scaffolds	1190:1225	engineered vascular tissue scaffolds	1190:1225	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	9	theme	engineered	1190:1199	arg1	promise					1179:1185	their promise	1173:1185	their promise	1173:1185	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	10	theme	tissue	1210:1215	arg1	scaffolds					1217:1225	engineered vascular tissue scaffolds	1190:1225	engineered vascular tissue scaffolds	1190:1225	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	10	theme	tissue	1210:1215	arg1	promise					1179:1185	their promise	1173:1185	their promise	1173:1185	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	0	11	theme	vascular	64:71	arg1	scaffold					91:98	vascular tissue-engineered scaffold	64:98	vascular tissue-engineered scaffold for promoting endothelial cell proliferation	64:143	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	2	12	theme	tissue	319:324	arg1	scaffold					326:333	tissue scaffold	319:333	tissue scaffold for promoting EC proliferation and vessel endothelialization	319:394	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	4	13	theme	nanofibrous	748:758	arg1	structures					760:769	various nanofibrous structures	740:769	various nanofibrous structures	740:769	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	2	14	theme	synthesized	505:515	arg1	materials					517:525	the synthesized materials	501:525	the synthesized materials	501:525	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	2	15	theme	vessel	370:375	arg1	endothelialization					377:394	vessel endothelialization	370:394	vessel endothelialization	370:394	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	5	16	theme	nanofibrous	971:981	arg1	scaffolds					983:991	the nanofibrous scaffolds	967:991	the nanofibrous scaffolds grafted by oHAs	967:1007	The results showed that the nanofibrous scaffolds grafted by oHAs promoted EC proliferation whereas high molecular weight HA inhibited proliferation.					
31427006	0	17	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	2	18	dep	engineered	300:309	arg1	develop					311:317	develop	311:317	develop tissue scaffold for promoting EC proliferation and vessel endothelialization	311:394	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	0	19	theme	collagen	41:48	arg1	nanofibers					50:59	collagen nanofibers	41:59	collagen nanofibers	41:59	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	4	20	theme	tissue	923:928	arg1	engineering					930:940	vascular tissue engineering	914:940	vascular tissue engineering	914:940	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	4	21	theme	nanofibrous	848:858	arg1	scaffolds					860:868	these nanofibrous scaffolds	842:868	these nanofibrous scaffolds	842:868	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	2	22	theme	biological	476:485	arg1	properties					487:496	the biological properties	472:496	the biological properties of the synthesized materials	472:525	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	6	23	contain	had	1107:1109	arg2	degree					1125:1130	no detectable degree	1111:1130	no detectable degree of hemolysis and coagulation	1111:1159	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	23	contain	had	1107:1109	arg1	scaffolds					1097:1105	The scaffolds	1093:1105	The scaffolds	1093:1105	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	1	24	theme	Hyaluronic	146:155	arg1	oHAs					180:183	oHAs	180:183	oHAs	180:183	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	1	24	theme	Hyaluronic	146:155	arg1	oligosaccharides					162:177	Hyaluronic acid oligosaccharides	146:177	Hyaluronic acid oligosaccharides (oHAs)	146:184	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	1	25	theme	cell	249:252	arg1	proliferation					259:271	vascular endothelial cell (EC) proliferation	228:271	vascular endothelial cell (EC) proliferation	228:271	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	3	26	gly	glycosylated	639:650	arg1	collagens					652:660	glycosylated collagens	639:660	glycosylated collagens	639:660	Firstly, oHAs were successfully prepared and conjugated with collagen to construct glycosylated collagens by reductive amination.					
31427006	4	27	theme	vascular	914:921	arg1	engineering					930:940	vascular tissue engineering	914:940	vascular tissue engineering	914:940	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	1	28	theme	vascular	228:235	arg1	cell					249:252	vascular endothelial cell	228:252	vascular endothelial cell (EC) proliferation	228:271	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	1	28	theme	vascular	228:235	arg1	EC					255:256	EC	255:256	EC	255:256	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	4	29	theme	various	740:746	arg1	structures					760:769	various nanofibrous structures	740:769	various nanofibrous structures	740:769	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	6	30	theme	detectable	1114:1123	arg1	degree					1125:1130	no detectable degree	1111:1130	no detectable degree of hemolysis and coagulation	1111:1159	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	0	31	theme	tissue-engineered	73:89	arg1	scaffold					91:98	vascular tissue-engineered scaffold	64:98	vascular tissue-engineered scaffold for promoting endothelial cell proliferation	64:143	Hyaluronic acid oligosaccharide-modified collagen nanofibers as vascular tissue-engineered scaffold for promoting endothelial cell proliferation.					
31427006	1	32	theme	acid	157:160	arg1	oHAs					180:183	oHAs	180:183	oHAs	180:183	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	1	32	theme	acid	157:160	arg1	oligosaccharides					162:177	Hyaluronic acid oligosaccharides	146:177	Hyaluronic acid oligosaccharides (oHAs)	146:184	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	2	33	theme	materials	517:525	arg1	properties					487:496	the biological properties	472:496	the biological properties of the synthesized materials	472:525	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	3	34	theme	reductive	665:673	arg1	amination					675:683	reductive amination	665:683	reductive amination	665:683	Firstly, oHAs were successfully prepared and conjugated with collagen to construct glycosylated collagens by reductive amination.					
31427006	2	35	theme	different	397:405	arg1	sizes					407:411	different sizes	397:411	different sizes of oHAs	397:419	To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis.					
31427006	3	36	theme	glycosylated	639:650	arg1	collagens					652:660	glycosylated collagens	639:660	glycosylated collagens	639:660	Firstly, oHAs were successfully prepared and conjugated with collagen to construct glycosylated collagens by reductive amination.					
31427006	4	37	theme	EC	826:827	arg1	responses					829:837	EC responses	826:837	EC responses on these nanofibrous scaffolds	826:868	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	4	38	theme	glycosylated	690:701	arg1	collagens					703:711	The glycosylated collagens	686:711	The glycosylated collagens	686:711	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	5	39	theme	EC	1018:1019	arg1	proliferation					1021:1033	EC proliferation	1018:1033	EC proliferation	1018:1033	The results showed that the nanofibrous scaffolds grafted by oHAs promoted EC proliferation whereas high molecular weight HA inhibited proliferation.					
31427006	5	40	theme	high	1043:1046	arg1	weight					1058:1063	high molecular weight	1043:1063	high molecular weight	1043:1063	The results showed that the nanofibrous scaffolds grafted by oHAs promoted EC proliferation whereas high molecular weight HA inhibited proliferation.					
31427006	4	41	from	morphology	781:790	arg1	scaffolds					860:868	these nanofibrous scaffolds	842:868	these nanofibrous scaffolds	842:868	The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering.					
31427006	1	42	theme	endothelial	237:247	arg1	cell					249:252	vascular endothelial cell	228:252	vascular endothelial cell (EC) proliferation	228:271	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	1	42	theme	endothelial	237:247	arg1	EC					255:256	EC	255:256	EC	255:256	Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization.					
31427006	6	43	theme	vascular	1201:1208	arg1	scaffolds					1217:1225	engineered vascular tissue scaffolds	1190:1225	engineered vascular tissue scaffolds	1190:1225	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	43	theme	vascular	1201:1208	arg1	promise					1179:1185	their promise	1173:1185	their promise	1173:1185	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	44	theme	hemolysis	1135:1143	arg1	degree					1125:1130	no detectable degree	1111:1130	no detectable degree of hemolysis and coagulation	1111:1159	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
31427006	6	45	theme	coagulation	1149:1159	arg1	degree					1125:1130	no detectable degree	1111:1130	no detectable degree of hemolysis and coagulation	1111:1159	The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.					
30563843	9	0	theme	hormone	1745:1751	arg1	pocket					1761:1766	AGT's hormone binding pocket	1739:1766	AGT's hormone binding pocket	1739:1766	These insights provide a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket.					
30563843	4	1	theme	allosteric	781:790	arg1	mechanism					792:800	a tail-into-mouth allosteric mechanism	763:800	a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins	763:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	4	2	theme	N	819:819	arg1	terminus					821:828	the N terminus	815:828	the N terminus	815:828	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	1	3	theme	fluid	165:169	arg1	balance					171:177	fluid balance	165:177	fluid balance	165:177	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	4	4	from	site	876:879	arg1	serpins					890:896	other serpins	884:896	other serpins	884:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	2	5	from	cleavage	195:202	arg1	terminus					244:251	the N terminus	238:251	the N terminus of angiotensinogen (AGT)	238:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	3	6	theme	AGT	590:592	arg1	structures					424:433	the crystal structures	412:433	the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	412:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	4	7	theme	tail-into-mouth	765:779	arg1	mechanism					792:800	a tail-into-mouth allosteric mechanism	763:800	a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins	763:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	2	8	theme	detailed	334:341	arg1	unclear					387:393	unclear	387:393	unclear	387:393	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	8	theme	detailed	334:341	arg1	mechanism					353:361	the detailed molecular mechanism	330:361	the detailed molecular mechanism underlying this step	330:382	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	8	9	theme	concerted	1522:1530	arg1	movements					1532:1540	concerted movements	1522:1540	concerted movements in the N-terminal tail and in its main body to modulate angiotensin release	1522:1616	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	3	10	with	AGT	525:527	arg1	renin					509:513	renin	509:513	renin (2.55 Å)	509:522	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	10	with	AGT	525:527	arg1	Å					521:521	2.55 Å	516:521	2.55 Å	516:521	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	11	theme	AGT	525:527	arg1	structures					424:433	the crystal structures	412:433	the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	412:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	2	12	theme	rate-limiting	285:297	arg1	cleavage					195:202	Renin-mediated cleavage	180:202	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	180:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	12	theme	rate-limiting	285:297	arg1	step					299:302	the rate-limiting step	281:302	the rate-limiting step of this cascade	281:318	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	7	13	theme	angiotensin	1292:1302	arg1	production					1278:1287	renin-mediated production	1263:1287	renin-mediated production of angiotensin I	1263:1304	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	7	14	theme	residues	1350:1357	arg1	components					1370:1379	glycan components	1363:1379	glycan components outside renin's active-site cleft	1363:1413	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	7	14	theme	residues	1350:1357	arg1	interactions					1323:1334	interactions	1323:1334	interactions of amino acid residues	1323:1357	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	7	15	theme	acid	1345:1348	arg1	residues					1350:1357	amino acid residues	1339:1357	amino acid residues	1339:1357	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	2	16	theme	molecular	343:351	arg1	unclear					387:393	unclear	387:393	unclear	387:393	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	16	theme	molecular	343:351	arg1	mechanism					353:361	the detailed molecular mechanism	330:361	the detailed molecular mechanism underlying this step	330:382	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	8	17	theme	angiotensin	1598:1608	arg1	release					1610:1616	angiotensin release	1598:1616	angiotensin release	1598:1616	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	8	18	theme	unique	1454:1459	arg1	features					1468:1475	unique serpin features	1454:1475	unique serpin features	1454:1475	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	9	19	theme	agents	1684:1689	arg1	development					1669:1679	the development	1665:1679	the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket	1665:1766	These insights provide a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket.					
30563843	3	20	theme	complex	496:502	arg1	structures					424:433	the crystal structures	412:433	the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	412:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	9	21	theme	angiotensin	1706:1716	arg1	release					1718:1724	angiotensin release	1706:1724	angiotensin release	1706:1724	These insights provide a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket.					
30563843	3	22	theme	crystal	416:422	arg1	structures					424:433	the crystal structures	412:433	the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	412:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	4	23	theme	hormone-binding	860:874	arg1	site					876:879	a hormone-binding site	858:879	a hormone-binding site on other serpins	858:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	3	24	theme	N-terminal	609:618	arg1	peptide					632:638	the N-terminal angiotensin peptide	605:638	the N-terminal angiotensin peptide	605:638	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	1	25	theme	renin-angiotensin	85:101	arg1	system					124:129	a hormone system	114:129	a hormone system that regulates blood pressure and fluid balance	114:177	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	1	25	theme	renin-angiotensin	85:101	arg1	cascade					103:109	The renin-angiotensin cascade	81:109	The renin-angiotensin cascade	81:109	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	2	26	from	terminus	244:251	arg1	cleavage					195:202	Renin-mediated cleavage	180:202	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	180:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	26	from	terminus	244:251	arg1	step					299:302	the rate-limiting step	281:302	the rate-limiting step of this cascade	281:318	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	26	from	terminus	244:251	arg1	peptide					225:231	the angiotensin I peptide	207:231	the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	207:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	5	27	theme	N-terminal	932:941	arg1	tail					943:946	the N-terminal tail	928:946	the N-terminal tail	928:946	These changes fully extended the N-terminal tail, with the scissile bond for angiotensin release docked in renin's active site.					
30563843	3	28	theme	angiotensin	620:630	arg1	peptide					632:638	the N-terminal angiotensin peptide	605:638	the N-terminal angiotensin peptide	605:638	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	9	29	theme	structural	1644:1653	arg1	basis					1655:1659	a structural basis	1642:1659	a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket	1642:1766	These insights provide a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket.					
30563843	2	30	theme	cascade	312:318	arg1	cleavage					195:202	Renin-mediated cleavage	180:202	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	180:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	30	theme	cascade	312:318	arg1	step					299:302	the rate-limiting step	281:302	the rate-limiting step of this cascade	281:318	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	0	31	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis for the specificity of renin-mediated angiotensinogen cleavage	0:78	Structural basis for the specificity of renin-mediated angiotensinogen cleavage.					
30563843	6	32	theme	terminus	1046:1053	arg1	Insertion					1027:1035	Insertion	1027:1035	Insertion of the N terminus into this pocket	1027:1070	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	6	33	theme	key	1147:1149	arg1	interactions					1151:1162	key interactions	1147:1162	key interactions with renin	1147:1173	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	3	34	theme	reactive	544:551	arg1	Å					576:576	RCL; 2.97 Å	566:576	RCL; 2.97 Å	566:576	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	34	theme	reactive	544:551	arg1	loop					560:563	its reactive center loop	540:563	its reactive center loop (RCL; 2.97 Å)	540:577	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	6	35	theme	H	1114:1114	arg1	unwinding					1095:1103	a complete unwinding	1084:1103	a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface	1084:1212	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	6	36	theme	N	1044:1044	arg1	terminus					1046:1053	the N terminus	1040:1053	the N terminus	1040:1053	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	7	37	theme	renin-mediated	1263:1276	arg1	production					1278:1287	renin-mediated production	1263:1287	renin-mediated production of angiotensin I	1263:1304	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	3	38	theme	human	451:455	arg1	resolution					469:478	2.30 Å resolution	462:478	2.30 Å resolution	462:478	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	38	theme	human	451:455	arg1	AGT					457:459	glycosylated human AGT	438:459	glycosylated human AGT (2.30 Å resolution)	438:479	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	8	39	theme	serpin	1461:1466	arg1	features					1468:1475	unique serpin features	1454:1475	unique serpin features	1454:1475	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	1	40	theme	hormone	116:122	arg1	system					124:129	a hormone system	114:129	a hormone system that regulates blood pressure and fluid balance	114:177	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	1	40	theme	hormone	116:122	arg1	cascade					103:109	The renin-angiotensin cascade	81:109	The renin-angiotensin cascade	81:109	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	3	41	theme	AGT	457:459	arg1	structures					424:433	the crystal structures	412:433	the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	412:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	42	with	complex	496:502	arg1	renin					509:513	renin	509:513	renin (2.55 Å)	509:522	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	42	with	complex	496:502	arg1	Å					521:521	2.55 Å	516:521	2.55 Å	516:521	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	8	43	theme	hormone	1481:1487	arg1	delivery					1489:1496	hormone delivery	1481:1496	hormone delivery	1481:1496	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	4	44	theme	equivalent	844:853	arg1	pocket					837:842	a pocket	835:842	a pocket equivalent to a hormone-binding site on other serpins	835:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	5	45	theme	angiotensin	976:986	arg1	release					988:994	angiotensin release	976:994	angiotensin release docked in renin's active site	976:1024	These changes fully extended the N-terminal tail, with the scissile bond for angiotensin release docked in renin's active site.					
30563843	4	46	theme	conformational	716:729	arg1	changes					731:737	profound conformational changes	707:737	profound conformational changes	707:737	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	7	47	theme	glycan	1363:1368	arg1	components					1370:1379	glycan components	1363:1379	glycan components outside renin's active-site cleft	1363:1413	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	3	48	with	AGT	457:459	arg1	renin					509:513	renin	509:513	renin (2.55 Å)	509:522	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	48	with	AGT	457:459	arg1	Å					521:521	2.55 Å	516:521	2.55 Å	516:521	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	2	49	theme	Renin-mediated	180:193	arg1	cleavage					195:202	Renin-mediated cleavage	180:202	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	180:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	49	theme	Renin-mediated	180:193	arg1	step					299:302	the rate-limiting step	281:302	the rate-limiting step of this cascade	281:318	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	50	theme	N	242:242	arg1	terminus					244:251	the N terminus	238:251	the N terminus of angiotensinogen (AGT)	238:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	3	51	theme	glycosylated	438:449	arg1	resolution					469:478	2.30 Å resolution	462:478	2.30 Å resolution	462:478	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	51	theme	glycosylated	438:449	arg1	AGT					457:459	glycosylated human AGT	438:459	glycosylated human AGT (2.30 Å resolution)	438:479	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	0	52	theme	angiotensinogen	55:69	arg1	cleavage					71:78	renin-mediated angiotensinogen cleavage	40:78	renin-mediated angiotensinogen cleavage	40:78	Structural basis for the specificity of renin-mediated angiotensinogen cleavage.					
30563843	6	53	theme	binding	1196:1202	arg1	interface					1204:1212	the complementary binding interface	1178:1212	the complementary binding interface	1178:1212	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	3	54	gly	glycosylated	438:449	arg1	resolution					469:478	2.30 Å resolution	462:478	2.30 Å resolution	462:478	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	54	gly	glycosylated	438:449	arg1	AGT					457:459	glycosylated human AGT	438:459	glycosylated human AGT (2.30 Å resolution)	438:479	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	7	55	theme	amino	1339:1343	arg1	residues					1350:1357	amino acid residues	1339:1357	amino acid residues	1339:1357	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	3	56	theme	Å	467:467	arg1	resolution					469:478	2.30 Å resolution	462:478	2.30 Å resolution	462:478	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	56	theme	Å	467:467	arg1	AGT					457:459	glycosylated human AGT	438:459	glycosylated human AGT (2.30 Å resolution)	438:479	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	0	57	theme	renin-mediated	40:53	arg1	cleavage					71:78	renin-mediated angiotensinogen cleavage	40:78	renin-mediated angiotensinogen cleavage	40:78	Structural basis for the specificity of renin-mediated angiotensinogen cleavage.					
30563843	6	58	theme	AGT	1119:1121	arg1	H					1114:1114	helix H	1108:1114	helix H	1108:1114	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	5	59	theme	active	1014:1019	arg1	site					1021:1024	renin's active site	1006:1024	renin's active site	1006:1024	These changes fully extended the N-terminal tail, with the scissile bond for angiotensin release docked in renin's active site.					
30563843	2	60	theme	peptide	225:231	arg1	cleavage					195:202	Renin-mediated cleavage	180:202	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	180:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	2	60	theme	peptide	225:231	arg1	step					299:302	the rate-limiting step	281:302	the rate-limiting step of this cascade	281:318	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	3	61	theme	spent	584:588	arg1	AGT					590:592	spent AGT	584:592	spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	584:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	61	theme	spent	584:588	arg1	Å					658:658	2.63 Å	653:658	2.63 Å	653:658	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	62	theme	RCL	566:568	arg1	Å					576:576	RCL; 2.97 Å	566:576	RCL; 2.97 Å	566:576	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	62	theme	RCL	566:568	arg1	loop					560:563	its reactive center loop	540:563	its reactive center loop (RCL; 2.97 Å)	540:577	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	9	63	theme	binding	1753:1759	arg1	pocket					1761:1766	AGT's hormone binding pocket	1739:1766	AGT's hormone binding pocket	1739:1766	These insights provide a structural basis for the development of agents that attenuate angiotensin release by targeting AGT's hormone binding pocket.					
30563843	6	64	theme	complete	1086:1093	arg1	unwinding					1095:1103	a complete unwinding	1084:1103	a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface	1084:1212	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	8	65	from	movements	1532:1540	arg1	body					1581:1584	its main body to modulate angiotensin release	1572:1616	its main body to modulate angiotensin release	1572:1616	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	8	65	from	movements	1532:1540	arg1	tail					1560:1563	the N-terminal tail	1545:1563	the N-terminal tail	1545:1563	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	2	66	theme	I	223:223	arg1	peptide					225:231	the angiotensin I peptide	207:231	the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	207:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	6	67	theme	helix	1108:1112	arg1	H					1114:1114	helix H	1108:1114	helix H	1108:1114	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	4	68	theme	profound	707:714	arg1	changes					731:737	profound conformational changes	707:737	profound conformational changes	707:737	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	8	69	theme	N-terminal	1549:1558	arg1	tail					1560:1563	the N-terminal tail	1545:1563	the N-terminal tail	1545:1563	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	6	70	theme	complementary	1182:1194	arg1	interface					1204:1212	the complementary binding interface	1178:1212	the complementary binding interface	1178:1212	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	3	71	attach	removed	644:650	arg1	AGT					590:592	spent AGT	584:592	spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å)	584:659	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	71	attach	removed	644:650	arg2	peptide					632:638	the N-terminal angiotensin peptide	605:638	the N-terminal angiotensin peptide	605:638	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	71	attach	removed	644:650	arg1	Å					658:658	2.63 Å	653:658	2.63 Å	653:658	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	2	72	theme	angiotensin	211:221	arg1	peptide					225:231	the angiotensin I peptide	207:231	the angiotensin I peptide from the N terminus of angiotensinogen (AGT)	207:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	7	73	theme	kinetic	1231:1237	arg1	analyses					1239:1246	kinetic analyses	1231:1246	kinetic analyses	1231:1246	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	1	74	theme	blood	146:150	arg1	pressure					152:159	blood pressure	146:159	blood pressure	146:159	The renin-angiotensin cascade is a hormone system that regulates blood pressure and fluid balance.					
30563843	6	75	with	interactions	1151:1162	arg1	renin					1169:1173	renin	1169:1173	renin	1169:1173	Insertion of the N terminus into this pocket accompanied a complete unwinding of helix H of AGT, which, in turn, formed key interactions with renin in the complementary binding interface.					
30563843	5	76	theme	scissile	958:965	arg1	bond					967:970	the scissile bond	954:970	the scissile bond for angiotensin release docked in renin's active site	954:1024	These changes fully extended the N-terminal tail, with the scissile bond for angiotensin release docked in renin's active site.					
30563843	4	77	theme	other	884:888	arg1	serpins					890:896	other serpins	884:896	other serpins	884:896	These structures revealed that AGT undergoes profound conformational changes and binds renin through a tail-into-mouth allosteric mechanism that inserts the N terminus into a pocket equivalent to a hormone-binding site on other serpins.					
30563843	7	78	theme	active-site	1397:1407	arg1	cleft					1409:1413	renin's active-site cleft	1389:1413	renin's active-site cleft	1389:1413	Mutagenesis and kinetic analyses confirmed that renin-mediated production of angiotensin I is controlled by interactions of amino acid residues and glycan components outside renin's active-site cleft.					
30563843	2	79	theme	angiotensinogen	256:270	arg1	terminus					244:251	the N terminus	238:251	the N terminus of angiotensinogen (AGT)	238:276	Renin-mediated cleavage of the angiotensin I peptide from the N terminus of angiotensinogen (AGT) is the rate-limiting step of this cascade; however, the detailed molecular mechanism underlying this step is unclear.					
30563843	8	80	theme	main	1576:1579	arg1	body					1581:1584	its main body to modulate angiotensin release	1572:1616	its main body to modulate angiotensin release	1572:1616	Our findings indicate that AGT adapts unique serpin features for hormone delivery and binds renin through concerted movements in the N-terminal tail and in its main body to modulate angiotensin release.					
30563843	0	81	theme	cleavage	71:78	arg1	specificity					25:35	the specificity	21:35	the specificity of renin-mediated angiotensinogen cleavage	21:78	Structural basis for the specificity of renin-mediated angiotensinogen cleavage.					
30563843	3	82	theme	center	553:558	arg1	Å					576:576	RCL; 2.97 Å	566:576	RCL; 2.97 Å	566:576	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	82	theme	center	553:558	arg1	loop					560:563	its reactive center loop	540:563	its reactive center loop (RCL; 2.97 Å)	540:577	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	83	theme	encounter	486:494	arg1	complex					496:502	its encounter complex	482:502	its encounter complex with renin (2.55 Å)	482:522	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	84	with	AGT	590:592	arg1	renin					509:513	renin	509:513	renin (2.55 Å)	509:522	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30563843	3	84	with	AGT	590:592	arg1	Å					521:521	2.55 Å	516:521	2.55 Å	516:521	Here, we solved the crystal structures of glycosylated human AGT (2.30 Å resolution), its encounter complex with renin (2.55 Å), AGT cleaved in its reactive center loop (RCL; 2.97 Å), and spent AGT from which the N-terminal angiotensin peptide was removed (2.63 Å).					
30177191	7	0	theme	structural	1040:1049	arg1	characterisation					1051:1066	The structural characterisation	1036:1066	The structural characterisation of the optimised film	1036:1088	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	0	1	from	effect	14:19	arg1	barrier					55:61	physical, optical, barrier and mechanical properties	36:87	barrier	55:61	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	0	1	from	effect	14:19	arg1	properties					78:87	physical, optical, barrier and mechanical properties	36:87	properties	78:87	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	7	2	theme	optimised	1075:1083	arg1	film					1085:1088	the optimised film	1071:1088	the optimised film	1071:1088	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	1	3	theme	based	184:188	arg1	film					214:217	based biodegradable composite film	184:217	based biodegradable composite film	184:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	3	4	theme	tensile	601:607	arg1	strength					609:616	maximization tensile strength	588:616	maximization tensile strength	588:616	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	1	5	theme	barrier	276:282	arg1	properties					284:293	barrier properties	276:293	barrier properties	276:293	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	5	6	theme	PS	793:794	arg1	%					820:820	3.7%	817:820	3.7%	817:820	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	6	theme	PS	793:794	arg1	value					784:788	The optimum value	772:788	The optimum value of PS, GG and glycerol	772:811	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	6	theme	PS	793:794	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	1	7	theme	biodegradable	190:202	arg1	film					214:217	based biodegradable composite film	184:217	based biodegradable composite film	184:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	0	8	theme	mechanical	67:76	arg1	properties					78:87	physical, optical, barrier and mechanical properties	36:87	properties	78:87	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	5	9	theme	solids	846:851	arg1	%					820:820	3.7%	817:820	3.7%	817:820	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	9	theme	solids	846:851	arg1	%					826:826	0.4%	823:826	0.4%	823:826	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	9	theme	solids	846:851	arg1	%					834:834	15%	832:834	15% (of total solids)	832:852	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	9	theme	solids	846:851	arg1	value					784:788	The optimum value	772:788	The optimum value of PS, GG and glycerol	772:811	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	9	theme	solids	846:851	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	4	10	theme	film	667:670	arg1	solution					680:687	film forming solution	667:687	film forming solution	667:687	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	1	11	theme	composite	204:212	arg1	film					214:217	based biodegradable composite film	184:217	based biodegradable composite film	184:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	3	12	theme	percentage	622:631	arg1	elongation					633:642	percentage elongation	622:642	percentage elongation	622:642	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	6	13	theme	significant	1009:1019	arg1	effect					1021:1026	any statistically significant effect	991:1026	any statistically significant effect on WVP	991:1033	All the independent variables depicted significant effect on the responses (p < 0.05) except starch which did not exhibit any statistically significant effect on WVP.					
30177191	4	14	theme	solution	680:687	arg1	Viscosity					654:662	Viscosity	654:662	Viscosity of film forming solution	654:687	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	2	15	theme	desirability	344:355	arg1	function					357:364	desirability function	344:364	desirability function	344:364	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	4	16	theme	forming	672:678	arg1	solution					680:687	film forming solution	667:687	film forming solution	667:687	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	6	17	from	effect	1021:1026	arg1	WVP					1031:1033	WVP	1031:1033	WVP	1031:1033	All the independent variables depicted significant effect on the responses (p < 0.05) except starch which did not exhibit any statistically significant effect on WVP.					
30177191	1	18	theme	potato	147:152	arg1	starch					154:159	potato starch	147:159	potato starch (PS)	147:164	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	1	18	theme	potato	147:152	arg1	PS					162:163	PS	162:163	PS	162:163	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	1	19	theme	starch	154:159	arg1	composition					132:142	The composition	128:142	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film	128:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	4	20	theme	films	745:749	arg1	thickness					690:698	thickness	690:698	thickness	690:698	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	4	20	theme	films	745:749	arg1	content					710:716	moisture content	701:716	moisture content	701:716	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	4	20	theme	films	745:749	arg1	index					732:736	whiteness index	722:736	whiteness index of the films	722:749	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	2	21	theme	composite	318:326	arg1	design					328:333	The rotatable central composite design	296:333	The rotatable central composite design applying desirability function	296:364	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	0	22	theme	starch	99:104	arg1	film					122:125	potato starch based composite film	92:125	potato starch based composite film	92:125	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	7	23	theme	developed	1175:1183	arg1	film					1185:1188	the developed film	1171:1188	the developed film	1171:1188	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	1	24	dep	starch	154:159	arg1	film					214:217	based biodegradable composite film	184:217	based biodegradable composite film	184:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	6	25	theme	independent	877:887	arg1	variables					889:897	All the independent variables	869:897	All the independent variables	869:897	All the independent variables depicted significant effect on the responses (p < 0.05) except starch which did not exhibit any statistically significant effect on WVP.					
30177191	0	26	theme	potato	92:97	arg1	film					122:125	potato starch based composite film	92:125	potato starch based composite film	92:125	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	3	27	theme	yellowness	536:545	arg1	index					547:551	yellowness index	536:551	yellowness index	536:551	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	7	28	theme	film	1085:1088	arg1	characterisation					1051:1066	The structural characterisation	1036:1066	The structural characterisation of the optimised film	1036:1088	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	5	29	theme	optimum	776:782	arg1	%					820:820	3.7%	817:820	3.7%	817:820	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	29	theme	optimum	776:782	arg1	value					784:788	The optimum value	772:788	The optimum value of PS, GG and glycerol	772:811	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	29	theme	optimum	776:782	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	0	30	theme	gum	29:31	arg1	effect					14:19	the effect	10:19	the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film	10:125	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	0	31	theme	composite	112:120	arg1	film					122:125	potato starch based composite film	92:125	potato starch based composite film	92:125	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	0	32	dep	physical	36:43	arg1	optical					46:52	optical	46:52	optical	46:52	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	7	33	theme	semi-crystalline	1144:1159	arg1	nature					1161:1166	the semi-crystalline nature	1140:1166	the semi-crystalline nature of the developed film	1140:1188	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	0	34	theme	guar	24:27	arg1	gum					29:31	guar gum	24:31	guar gum	24:31	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	0	35	theme	based	106:110	arg1	film					122:125	potato starch based composite film	92:125	potato starch based composite film	92:125	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	2	36	theme	glycerol	432:439	arg1	effect					389:394	the effect	385:394	the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%)	385:448	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	1	37	theme	guar	170:173	arg1	GG					180:181	GG	180:181	GG	180:181	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	1	37	theme	guar	170:173	arg1	gum					175:177	guar gum	170:177	guar gum (GG)	170:182	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	0	38	theme	physical	36:43	arg1	barrier					55:61	physical, optical, barrier and mechanical properties	36:87	barrier	55:61	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	2	39	theme	PS	399:400	arg1	effect					389:394	the effect	385:394	the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%)	385:448	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	0	40	theme	film	122:125	arg1	barrier					55:61	physical, optical, barrier and mechanical properties	36:87	barrier	55:61	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	0	40	theme	film	122:125	arg1	properties					78:87	physical, optical, barrier and mechanical properties	36:87	properties	78:87	Modelling the effect of guar gum on physical, optical, barrier and mechanical properties of potato starch based composite film.					
30177191	3	41	theme	vapour	563:568	arg1	permeability					570:581	water vapour permeability	557:581	water vapour permeability	557:581	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	4	42	theme	whiteness	722:730	arg1	index					732:736	whiteness index	722:736	whiteness index of the films	722:749	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	2	43	used	used	370:373	arg2	design					328:333	The rotatable central composite design	296:333	The rotatable central composite design applying desirability function	296:364	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	5	44	theme	GG	797:798	arg1	%					820:820	3.7%	817:820	3.7%	817:820	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	44	theme	GG	797:798	arg1	value					784:788	The optimum value	772:788	The optimum value of PS, GG and glycerol	772:811	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	44	theme	GG	797:798	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	45	theme	total	840:844	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	7	46	theme	film	1185:1188	arg1	nature					1161:1166	the semi-crystalline nature	1140:1166	the semi-crystalline nature of the developed film	1140:1188	The structural characterisation of the optimised film was also analysed by FESEM and XRD which point to the semi-crystalline nature of the developed film.					
30177191	3	47	theme	solubility	515:524	arg1	opacity					527:533	opacity	527:533	opacity	527:533	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	47	theme	solubility	515:524	arg1	minimization					494:505	minimization	494:505	minimization of film solubility	494:524	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	47	theme	solubility	515:524	arg1	elongation					633:642	percentage elongation	622:642	percentage elongation	622:642	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	47	theme	solubility	515:524	arg1	index					547:551	yellowness index	536:551	yellowness index	536:551	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	47	theme	solubility	515:524	arg1	strength					609:616	maximization tensile strength	588:616	maximization tensile strength	588:616	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	47	theme	solubility	515:524	arg1	permeability					570:581	water vapour permeability	557:581	water vapour permeability	557:581	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	1	48	theme	gum	175:177	arg1	composition					132:142	The composition	128:142	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film	128:217	The composition of potato starch (PS) and guar gum (GG) based biodegradable composite film was optimised based on physical, optical, mechanical and barrier properties.					
30177191	5	49	theme	glycerol	804:811	arg1	%					820:820	3.7%	817:820	3.7%	817:820	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	49	theme	glycerol	804:811	arg1	value					784:788	The optimum value	772:788	The optimum value of PS, GG and glycerol	772:811	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	5	49	theme	glycerol	804:811	arg1	solids					846:851	total solids	840:851	total solids	840:851	The optimum value of PS, GG and glycerol was 3.7%, 0.4% and 15% (of total solids), respectively.					
30177191	3	50	theme	water	557:561	arg1	permeability					570:581	water vapour permeability	557:581	water vapour permeability	557:581	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	4	51	theme	moisture	701:708	arg1	content					710:716	moisture content	701:716	moisture content	701:716	Viscosity of film forming solution; thickness, moisture content and whiteness index of the films were also evaluated.					
30177191	2	52	theme	central	310:316	arg1	design					328:333	The rotatable central composite design	296:333	The rotatable central composite design applying desirability function	296:364	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	6	53	theme	significant	908:918	arg1	effect					920:925	significant effect	908:925	significant effect	908:925	All the independent variables depicted significant effect on the responses (p < 0.05) except starch which did not exhibit any statistically significant effect on WVP.					
30177191	2	54	theme	GG	414:415	arg1	effect					389:394	the effect	385:394	the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%)	385:448	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30177191	3	55	theme	maximization	588:599	arg1	strength					609:616	maximization tensile strength	588:616	maximization tensile strength	588:616	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	3	56	theme	film	510:513	arg1	solubility					515:524	film solubility	510:524	film solubility	510:524	The optimisation was performed considering minimization of film solubility, opacity, yellowness index and water vapour permeability; and maximization tensile strength and percentage elongation at break.					
30177191	2	57	theme	rotatable	300:308	arg1	design					328:333	The rotatable central composite design	296:333	The rotatable central composite design applying desirability function	296:364	The rotatable central composite design applying desirability function was used to assess the effect of PS (2.5-4.5%), GG (0.2-0.8%) and glycerol (15-35%).					
30659878	4	0	theme	percentage	960:969	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	1	theme	%	997:997	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	3	2	theme	liver	769:773	arg1	injury					775:780	the alcohol-induced liver injury	749:780	the alcohol-induced liver injury	749:780	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	0	3	with	mice	97:100	arg1	injury					129:134	alcohol-induced liver injury	107:134	alcohol-induced liver injury	107:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	4	4	theme	%	1004:1004	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	0	5	theme	alcohol-induced	107:121	arg1	injury					129:134	alcohol-induced liver injury	107:134	alcohol-induced liver injury	107:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	4	6	theme	magnetic	1114:1121	arg1	analysis					1139:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	3	7	theme	alcohol-induced	604:618	arg1	injury					626:631	alcohol-induced liver injury	604:631	alcohol-induced liver injury	604:631	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	4	8	theme	a	930:930	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	9	theme	nuclear	1106:1112	arg1	analysis					1139:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	2	10	theme	liver	296:300	arg1	injury					302:307	alcohol-induced liver injury	280:307	alcohol-induced liver injury	280:307	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	4	11	theme	%	1025:1025	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	0	12	theme	liver	123:127	arg1	injury					129:134	alcohol-induced liver injury	107:134	alcohol-induced liver injury	107:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	0	13	theme	modified	47:54	arg1	polysaccharides					56:70	modified polysaccharides	47:70	modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury	47:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	2	14	theme	alcohol-induced	280:294	arg1	injury					302:307	alcohol-induced liver injury	280:307	alcohol-induced liver injury	280:307	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	4	15	theme	%	1036:1036	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	16	theme	%	990:990	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	0	17	theme	Antioxidant	0:10	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.	0:135	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	4	18	theme	%	1018:1018	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	2	19	theme	structure	325:333	arg1	features					335:342	preliminary structure features	313:342	preliminary structure features	313:342	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	0	20	theme	hepatoprotective	16:31	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.	0:135	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	2	21	theme	preliminary	313:323	arg1	features					335:342	preliminary structure features	313:342	preliminary structure features	313:342	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	3	22	theme	functional	707:716	arg1	foods					718:722	functional foods	707:722	functional foods	707:722	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	22	theme	functional	707:716	arg1	references					664:673	references	664:673	references for the exploitation of MPCC	664:702	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	23	theme	serum	449:453	arg1	levels					461:466	the hepatic and serum lipid levels	433:466	levels	461:466	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	24	theme	natural	727:733	arg1	references					664:673	references	664:673	references for the exploitation of MPCC	664:702	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	24	theme	natural	727:733	arg1	drugs					735:739	natural drugs	727:739	natural drugs	727:739	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	2	25	from	effects	269:275	arg1	injury					302:307	alcohol-induced liver injury	280:307	alcohol-induced liver injury	280:307	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	2	25	from	effects	269:275	arg1	features					335:342	preliminary structure features	313:342	preliminary structure features	313:342	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	3	26	theme	antioxidant	488:498	arg1	activities					507:516	antioxidant enzyme activities	488:516	antioxidant enzyme activities	488:516	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	27	theme	lipid	455:459	arg1	levels					461:466	the hepatic and serum lipid levels	433:466	levels	461:466	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	28	dep	in	367:368	arg1	vivo					370:373	vivo	370:373	vivo	370:373	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	0	29	from	comatus	86:92	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.	0:135	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	0	29	from	comatus	86:92	arg1	polysaccharides					56:70	modified polysaccharides	47:70	modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury	47:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	0	29	from	comatus	86:92	arg1	mice					97:100	mice	97:100	mice with alcohol-induced liver injury	97:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	3	30	theme	enzyme	500:505	arg1	activities					507:516	antioxidant enzyme activities	488:516	antioxidant enzyme activities	488:516	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	31	theme	in	367:368	arg1	abilities					392:400	in vivo hepatoprotective abilities	367:400	in vivo hepatoprotective abilities	367:400	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	4	32	with	β-configuration	939:953	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	3	33	theme	alcohol	537:543	arg1	system					556:561	alcohol metabolism system	537:561	alcohol metabolism system	537:561	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	0	34	theme	polysaccharides	56:70	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.	0:135	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	3	35	theme	hepatoprotective	375:390	arg1	abilities					392:400	in vivo hepatoprotective abilities	367:400	in vivo hepatoprotective abilities	367:400	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	4	36	theme	resonance	1123:1131	arg1	analysis					1139:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	3	37	theme	metabolism	545:554	arg1	system					556:561	alcohol metabolism system	537:561	alcohol metabolism system	537:561	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	38	theme	alcohol-induced	753:767	arg1	injury					775:780	the alcohol-induced liver injury	749:780	the alcohol-induced liver injury	749:780	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	3	39	theme	MPCC	699:702	arg1	exploitation					683:694	the exploitation	679:694	the exploitation of MPCC	679:702	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	1	40	theme	modified	139:146	arg1	polysaccharide					148:161	A modified polysaccharide	137:161	A modified polysaccharide named MPCC from Coprinus comatus (MPCC)	137:201	A modified polysaccharide named MPCC from Coprinus comatus (MPCC) was obtained by the snailase hydrolysis.					
30659878	3	41	theme	liver	620:624	arg1	injury					626:631	alcohol-induced liver injury	604:631	alcohol-induced liver injury	604:631	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	1	42	theme	snailase	223:230	arg1	hydrolysis					232:241	the snailase hydrolysis	219:241	the snailase hydrolysis	219:241	A modified polysaccharide named MPCC from Coprinus comatus (MPCC) was obtained by the snailase hydrolysis.					
30659878	4	43	with	galactose	887:895	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	44	theme	gas	1042:1044	arg1	chromatography					1046:1059	gas chromatography	1042:1059	gas chromatography (GC)	1042:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	44	theme	gas	1042:1044	arg1	GC					1062:1063	GC	1062:1063	GC	1062:1063	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	45	dep	infrared	1085:1092	arg1	FT-IR					1095:1099	FT-IR	1095:1099	FT-IR	1095:1099	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	46	with	fucose	813:818	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	47	theme	NMR	1134:1136	arg1	analysis					1139:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	nuclear magnetic resonance (NMR) analysis	1106:1146	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	2	48	theme	hepatoprotective	252:267	arg1	effects					269:275	the hepatoprotective effects	248:275	the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features	248:342	And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated.					
30659878	3	49	theme	inflammatory	567:578	arg1	response					580:587	inflammatory response	567:587	inflammatory response	567:587	For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury.					
30659878	4	50	theme	%	1011:1011	arg1	composition					971:981	a percentage composition	958:981	a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC)	958:1064	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	0	51	theme	Coprinus	77:84	arg1	comatus					86:92	Coprinus comatus	77:92	Coprinus comatus in mice with alcohol-induced liver injury	77:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	0	52	from	activities	33:42	arg1	comatus					86:92	Coprinus comatus	77:92	Coprinus comatus in mice with alcohol-induced liver injury	77:134	Antioxidant and hepatoprotective activities of modified polysaccharides from Coprinus comatus in mice with alcohol-induced liver injury.					
30659878	4	53	with	glucose	907:913	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30659878	4	54	with	mannose	872:878	arg1	α-					932:933	the a α-	926:933	the a α-	926:933	Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and β-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.					
30981359	5	0	theme	1,5-linked-α-l-Araf	779:797	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	5	1	theme	glycosidic	688:697	arg1	bonds					699:703	the main glycosidic bonds	679:703	the main glycosidic bonds of MFP-2A	679:713	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	6	2	theme	inhibitory	968:977	arg1	activities					979:988	α-glucosidase inhibitory activities	954:988	α-glucosidase inhibitory activities	954:988	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	0	3	from	characterization	12:27	arg1	furetianus					115:124	Mallotus furetianus	106:124	Mallotus furetianus	106:124	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	5	4	theme	NMR	652:654	arg1	analysis					656:663	methylation and NMR analysis	636:663	analysis	656:663	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	7	5	theme	furetianus	1062:1071	arg1	Arg					1086:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	4	6	theme	molar	582:586	arg1	ratio					588:592	a molar ratio	580:592	a molar ratio of 3.05:2.84:1.84:0.86:1.41	580:620	The monosaccharide composition was consisted of mannose, galactose, arabinose, galacturonic acid and glucose with a molar ratio of 3.05:2.84:1.84:0.86:1.41.					
30981359	0	7	from	activities	71:80	arg1	furetianus					115:124	Mallotus furetianus	106:124	Mallotus furetianus	106:124	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	5	8	theme	1,4,6-linked-β-d-Galp	733:753	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	6	9	theme	α-glucosidase	954:966	arg1	activities					979:988	α-glucosidase inhibitory activities	954:988	α-glucosidase inhibitory activities	954:988	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	3	10	theme	average	427:433	arg1	weight					445:450	an average molecular weight	424:450	an average molecular weight of 25.96 KDa	424:463	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	1	11	attach	isolated	180:187	arg2	polysaccharide					138:151	A purified polysaccharide	127:151	A purified polysaccharide	127:151	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	1	11	attach	isolated	180:187	arg1	Mallotus					194:201	Mallotus	194:201	Mallotus	194:201	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	1	11	attach	isolated	180:187	arg1	Bail					215:218	Bail	215:218	Bail	215:218	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	0	12	theme	α-glucosidase	46:58	arg1	activities					71:80	α-glucosidase inhibitory activities	46:80	α-glucosidase inhibitory activities	46:80	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	3	13	with	heteropolysaccharide	398:417	arg1	weight					445:450	an average molecular weight	424:450	an average molecular weight of 25.96 KDa	424:463	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	3	14	theme	molecular	435:443	arg1	weight					445:450	an average molecular weight	424:450	an average molecular weight of 25.96 KDa	424:463	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	5	15	theme	1,4-linked-α-d-Glcp	844:862	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	4	16	theme	monosaccharide	470:483	arg1	composition					485:495	The monosaccharide composition	466:495	The monosaccharide composition	466:495	The monosaccharide composition was consisted of mannose, galactose, arabinose, galacturonic acid and glucose with a molar ratio of 3.05:2.84:1.84:0.86:1.41.					
30981359	5	17	theme	1,3,6-linked-β-d-Manp	756:776	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	0	18	theme	Preliminary	0:10	arg1	characterization					12:27	Preliminary characterization	0:27	Preliminary characterization	0:27	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	4	19	theme	galacturonic	545:556	arg1	acid					558:561	galacturonic acid	545:561	galacturonic acid	545:561	The monosaccharide composition was consisted of mannose, galactose, arabinose, galacturonic acid and glucose with a molar ratio of 3.05:2.84:1.84:0.86:1.41.					
30981359	5	20	theme	methylation	636:646	arg1	analysis					656:663	methylation and NMR analysis	636:663	analysis	656:663	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	2	21	theme	methylation	309:319	arg1	analysis					321:328	methylation analysis	309:328	methylation analysis	309:328	The primary structure characteristics were determined by HPGPC, HPLC, FT-IR, methylation analysis, NMR, SEM and AFM.					
30981359	7	22	theme	functional	1135:1144	arg1	MFP-2A					1041:1046	MFP-2A	1041:1046	MFP-2A from Mallotus furetianus (Bail) Muell Arg	1041:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	7	22	theme	functional	1135:1144	arg1	food					1146:1149	a potential anti-diabetic and functional food	1105:1149	a potential anti-diabetic and functional food	1105:1149	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	7	23	theme	anti-diabetic	1117:1129	arg1	MFP-2A					1041:1046	MFP-2A	1041:1046	MFP-2A from Mallotus furetianus (Bail) Muell Arg	1041:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	7	23	theme	anti-diabetic	1117:1129	arg1	food					1146:1149	a potential anti-diabetic and functional food	1105:1149	a potential anti-diabetic and functional food	1105:1149	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	6	24	theme	scavenging	931:940	arg1	ability					942:948	significant free radical scavenging ability	906:948	significant free radical scavenging ability	906:948	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	1	25	theme	purified	129:136	arg1	polysaccharide					138:151	A purified polysaccharide	127:151	A purified polysaccharide	127:151	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	3	26	theme	KDa	461:463	arg1	weight					445:450	an average molecular weight	424:450	an average molecular weight of 25.96 KDa	424:463	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	3	27	theme	homogeneous	386:396	arg1	MFP-2A					373:378	MFP-2A	373:378	MFP-2A	373:378	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	3	27	theme	homogeneous	386:396	arg1	heteropolysaccharide					398:417	a homogeneous heteropolysaccharide	384:417	a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa	384:463	The results showed that MFP-2A was a homogeneous heteropolysaccharide with an average molecular weight of 25.96 KDa.					
30981359	5	28	theme	MFP-2A	708:713	arg1	bonds					699:703	the main glycosidic bonds	679:703	the main glycosidic bonds of MFP-2A	679:713	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	6	29	theme	radical	923:929	arg1	ability					942:948	significant free radical scavenging ability	906:948	significant free radical scavenging ability	906:948	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	2	30	theme	structure	244:252	arg1	characteristics					254:268	The primary structure characteristics	232:268	The primary structure characteristics	232:268	The primary structure characteristics were determined by HPGPC, HPLC, FT-IR, methylation analysis, NMR, SEM and AFM.					
30981359	1	31	theme	Muell	221:225	arg1	Arg					227:229	Muell Arg	221:229	Mallotus furetianus (Bail) Muell Arg	194:229	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	6	32	theme	free	918:921	arg1	radical					923:929	significant free radical	906:929	significant free radical scavenging ability	906:948	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	2	33	theme	primary	236:242	arg1	characteristics					254:268	The primary structure characteristics	232:268	The primary structure characteristics	232:268	The primary structure characteristics were determined by HPGPC, HPLC, FT-IR, methylation analysis, NMR, SEM and AFM.					
30981359	5	34	theme	1,4-linked-α-d-GalAp	819:838	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	6	35	theme	significant	906:916	arg1	radical					923:929	significant free radical	906:929	significant free radical scavenging ability	906:948	Moreover, MFP-2A also exhibited significant free radical scavenging ability and α-glucosidase inhibitory activities in vitro.					
30981359	0	36	theme	inhibitory	60:69	arg1	activities					71:80	α-glucosidase inhibitory activities	46:80	α-glucosidase inhibitory activities	46:80	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	7	37	theme	potential	1107:1115	arg1	MFP-2A					1041:1046	MFP-2A	1041:1046	MFP-2A from Mallotus furetianus (Bail) Muell Arg	1041:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	7	37	theme	potential	1107:1115	arg1	food					1146:1149	a potential anti-diabetic and functional food	1105:1149	a potential anti-diabetic and functional food	1105:1149	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	1	38	dep	Mallotus	194:201	arg1	furetianus					203:212	Mallotus furetianus	194:212	Mallotus furetianus (Bail) Muell Arg	194:229	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	1	38	dep	Mallotus	194:201	arg1	Arg					227:229	Muell Arg	221:229	Mallotus furetianus (Bail) Muell Arg	194:229	A purified polysaccharide, designated as MFP-2A, was isolated from Mallotus furetianus (Bail) Muell Arg.					
30981359	7	39	from	Arg	1086:1088	arg1	MFP-2A					1041:1046	MFP-2A	1041:1046	MFP-2A from Mallotus furetianus (Bail) Muell Arg	1041:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	7	39	from	Arg	1086:1088	arg1	food					1146:1149	a potential anti-diabetic and functional food	1105:1149	a potential anti-diabetic and functional food	1105:1149	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	5	40	theme	main	683:686	arg1	bonds					699:703	the main glycosidic bonds	679:703	the main glycosidic bonds of MFP-2A	679:713	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	7	41	theme	Muell	1080:1084	arg1	Arg					1086:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	5	42	theme	1-linked-α-l-Araf	800:816	arg1	residues					864:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues	733:871	In addition, methylation and NMR analysis revealed that the main glycosidic bonds of MFP-2A were comprised of 1,4,6-linked-β-d-Galp, 1,3,6-linked-β-d-Manp, 1,5-linked-α-l-Araf, 1-linked-α-l-Araf, 1,4-linked-α-d-GalAp and 1,4-linked-α-d-Glcp residues.					
30981359	0	43	theme	polysaccharides	85:99	arg1	antioxidant					30:40	antioxidant	30:40	antioxidant	30:40	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	43	theme	polysaccharides	85:99	arg1	characterization					12:27	Preliminary characterization	0:27	Preliminary characterization	0:27	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	43	theme	polysaccharides	85:99	arg1	activities					71:80	α-glucosidase inhibitory activities	46:80	α-glucosidase inhibitory activities	46:80	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	44	from	furetianus	115:124	arg1	activities					71:80	α-glucosidase inhibitory activities	46:80	α-glucosidase inhibitory activities	46:80	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	44	from	furetianus	115:124	arg1	characterization					12:27	Preliminary characterization	0:27	Preliminary characterization	0:27	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	44	from	furetianus	115:124	arg1	antioxidant					30:40	antioxidant	30:40	antioxidant	30:40	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	0	44	from	furetianus	115:124	arg1	polysaccharides					85:99	polysaccharides	85:99	polysaccharides from Mallotus furetianus	85:124	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	7	45	theme	Bail	1074:1077	arg1	Arg					1086:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	0	46	from	antioxidant	30:40	arg1	furetianus					115:124	Mallotus furetianus	106:124	Mallotus furetianus	106:124	Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.					
30981359	7	47	theme	Mallotus	1053:1060	arg1	Arg					1086:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Mallotus furetianus (Bail) Muell Arg	1053:1088	Overall, these results demonstrated that MFP-2A from Mallotus furetianus (Bail) Muell Arg could serve as a potential anti-diabetic and functional food.					
30981359	4	48	theme	3.05:2.84:1.84:0.86:1.41	597:620	arg1	ratio					588:592	a molar ratio	580:592	a molar ratio of 3.05:2.84:1.84:0.86:1.41	580:620	The monosaccharide composition was consisted of mannose, galactose, arabinose, galacturonic acid and glucose with a molar ratio of 3.05:2.84:1.84:0.86:1.41.					
31590873	6	0	theme	light	1076:1080	arg1	diode					1097:1101	light light-emitting diode	1076:1101	light light-emitting diode (LED, 3.0 V)	1076:1114	More importantly, two series-wound devices were easy to light light-emitting diode (LED, 3.0 V).					
31590873	5	1	theme	supercapacitor	921:934	arg1	density					910:916	The energy density	899:916	The energy density of supercapacitor based on HPC	899:947	The energy density of supercapacitor based on HPC was comparable to or higher than that of commercially supercapacitors.					
31590873	5	1	theme	supercapacitor	921:934	arg1	comparable					953:962	comparable	953:962	comparable	953:962	The energy density of supercapacitor based on HPC was comparable to or higher than that of commercially supercapacitors.					
31590873	1	2	theme	citric	395:400	arg1	polysaccharides					329:343	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	2	theme	citric	395:400	arg1	acid					402:405	citric acid	395:405	citric acid	395:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	4	3	theme	stability	888:896	arg1	capacitance					817:827	a high specific capacitance	801:827	a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability	801:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	7	4	theme	energy	1220:1225	arg1	devices					1235:1241	low-cost and eco-friendly energy storage devices	1194:1241	low-cost and eco-friendly energy storage devices	1194:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	1	5	theme	activation	297:306	arg1	process					308:314	activation process	297:314	activation process	297:314	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	0	6	theme	citric	99:104	arg1	acid					106:109	citric acid	99:109	citric acid	99:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	6	theme	citric	99:104	arg1	polysaccharides					33:47	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	3	7	with	structures	563:572	arg1	macropores					579:588	macropores	579:588	macropores	579:588	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	7	with	structures	563:572	arg1	walls					600:604	mesopore walls	591:604	mesopore walls	591:604	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	7	with	structures	563:572	arg1	content					635:641	high oxygen content	623:641	high oxygen content	623:641	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	7	with	structures	563:572	arg1	micropores					607:616	micropores	607:616	micropores	607:616	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	1	8	attach	derived	316:322	arg1	polysaccharides					329:343	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	8	attach	derived	316:322	arg1	acid					402:405	citric acid	395:405	citric acid	395:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	8	attach	derived	316:322	arg2	carbonization					279:291	one-step carbonization	270:291	one-step carbonization	270:291	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	8	attach	derived	316:322	arg1	cellulose					359:367	carboxymethyl cellulose	345:367	carboxymethyl cellulose	345:367	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	8	attach	derived	316:322	arg1	cellulose					380:388	bacterial cellulose	370:388	bacterial cellulose	370:388	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	4	9	theme	three-electrode	752:766	arg1	system					768:773	a three-electrode system	750:773	a three-electrode system	750:773	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	2	10	theme	high	476:479	arg1	2490 m2 g-1					504:514	2490 m2 g-1	504:514	2490 m2 g-1	504:514	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	10	theme	high	476:479	arg1	area					498:501	high specific surface area	476:501	high specific surface area (2490 m2 g-1)	476:515	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	4	11	theme	specific	808:815	arg1	capacitance					817:827	a high specific capacitance	801:827	a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability	801:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	2	12	theme	unique	436:441	arg1	morphology					460:469	unique porous nanosheet morphology	436:469	unique porous nanosheet morphology	436:469	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	1	13	theme	hierarchical	190:201	arg1	materials					233:241	hierarchical composite porous carbon (HPC) materials	190:241	hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	190:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	7	14	theme	eco-friendly	1207:1218	arg1	devices					1235:1241	low-cost and eco-friendly energy storage devices	1194:1241	low-cost and eco-friendly energy storage devices	1194:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	2	15	theme	oxygen	526:531	arg1	content					533:539	rich oxygen content	521:539	rich oxygen content (7.3%)	521:546	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	15	theme	oxygen	526:531	arg1	%					545:545	7.3%	542:545	7.3%	542:545	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	4	16	theme	high	803:806	arg1	capacitance					817:827	a high specific capacitance	801:827	a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability	801:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	1	17	theme	composite	203:211	arg1	materials					233:241	hierarchical composite porous carbon (HPC) materials	190:241	hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	190:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	3	18	theme	developed	553:561	arg1	structures					563:572	The developed structures	549:572	The developed structures with macropores, mesopore walls, micropores, and high oxygen content	549:641	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	4	19	theme	cycling	880:886	arg1	stability					888:896	excellent cycling stability	870:896	excellent cycling stability	870:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	1	20	theme	porous	213:218	arg1	materials					233:241	hierarchical composite porous carbon (HPC) materials	190:241	hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	190:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	0	21	theme	Hierarchical	0:11	arg1	porous					13:18	Hierarchical porous	0:18	Hierarchical porous	0:18	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	4	22	theme	excellent	870:878	arg1	stability					888:896	excellent cycling stability	870:896	excellent cycling stability	870:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	6	23	dep	diode	1097:1101	arg1	LED					1104:1106	LED	1104:1106	LED	1104:1106	More importantly, two series-wound devices were easy to light light-emitting diode (LED, 3.0 V).					
31590873	1	24	theme	carbon	220:225	arg1	materials					233:241	hierarchical composite porous carbon (HPC) materials	190:241	hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	190:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	7	25	theme	promising	1170:1178	arg1	candidate					1180:1188	a promising candidate	1168:1188	a promising candidate for low-cost and eco-friendly energy storage devices	1168:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	7	25	theme	promising	1170:1178	arg1	material					1156:1163	the current material	1144:1163	the current material	1144:1163	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	4	26	theme	rate	848:851	arg1	performance					853:863	good rate performance	843:863	good rate performance	843:863	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	3	27	theme	mesopore	591:598	arg1	walls					600:604	mesopore walls	591:604	mesopore walls	591:604	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	4	28	theme	good	843:846	arg1	performance					853:863	good rate performance	843:863	good rate performance	843:863	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	2	29	theme	specific	481:488	arg1	2490 m2 g-1					504:514	2490 m2 g-1	504:514	2490 m2 g-1	504:514	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	29	theme	specific	481:488	arg1	area					498:501	high specific surface area	476:501	high specific surface area (2490 m2 g-1)	476:515	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	30	theme	surface	490:496	arg1	2490 m2 g-1					504:514	2490 m2 g-1	504:514	2490 m2 g-1	504:514	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	30	theme	surface	490:496	arg1	area					498:501	high specific surface area	476:501	high specific surface area (2490 m2 g-1)	476:515	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	5	31	theme	commercially	990:1001	arg1	supercapacitors					1003:1017	commercially supercapacitors	990:1017	commercially supercapacitors	990:1017	The energy density of supercapacitor based on HPC was comparable to or higher than that of commercially supercapacitors.					
31590873	4	32	theme	HPC	780:782	arg1	electrode					784:792	the HPC electrode	776:792	the HPC electrode	776:792	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	1	33	theme	HPC	228:230	arg1	materials					233:241	hierarchical composite porous carbon (HPC) materials	190:241	hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	190:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	0	34	theme	carboxymethyl	49:61	arg1	cellulose					63:71	carboxymethyl cellulose	49:71	carboxymethyl cellulose	49:71	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	34	theme	carboxymethyl	49:61	arg1	polysaccharides					33:47	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	1	35	theme	carboxymethyl	345:357	arg1	polysaccharides					329:343	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	35	theme	carboxymethyl	345:357	arg1	cellulose					359:367	carboxymethyl cellulose	345:367	carboxymethyl cellulose	345:367	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	7	36	theme	low-cost	1194:1201	arg1	devices					1235:1241	low-cost and eco-friendly energy storage devices	1194:1241	low-cost and eco-friendly energy storage devices	1194:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	3	37	theme	high	623:626	arg1	content					635:641	high oxygen content	623:641	high oxygen content	623:641	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	5	38	theme	energy	903:908	arg1	density					910:916	The energy density	899:916	The energy density of supercapacitor based on HPC	899:947	The energy density of supercapacitor based on HPC was comparable to or higher than that of commercially supercapacitors.					
31590873	5	38	theme	energy	903:908	arg1	comparable					953:962	comparable	953:962	comparable	953:962	The energy density of supercapacitor based on HPC was comparable to or higher than that of commercially supercapacitors.					
31590873	2	39	theme	nanosheet	450:458	arg1	morphology					460:469	unique porous nanosheet morphology	436:469	unique porous nanosheet morphology	436:469	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	40	theme	rich	521:524	arg1	content					533:539	rich oxygen content	521:539	rich oxygen content (7.3%)	521:546	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	2	40	theme	rich	521:524	arg1	%					545:545	7.3%	542:545	7.3%	542:545	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	3	41	theme	electrochemical	660:674	arg1	performance					676:686	excellent electrochemical performance	650:686	excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs)	650:744	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	42	theme	oxygen	628:633	arg1	content					635:641	high oxygen content	623:641	high oxygen content	623:641	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	1	43	theme	materials	233:241	arg1	performance					175:185	excellent supercapacitor performance	150:185	excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	150:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	7	44	theme	current	1148:1154	arg1	candidate					1180:1188	a promising candidate	1168:1188	a promising candidate for low-cost and eco-friendly energy storage devices	1168:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	7	44	theme	current	1148:1154	arg1	material					1156:1163	the current material	1144:1163	the current material	1144:1163	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	4	45	theme	performance	853:863	arg1	capacitance					817:827	a high specific capacitance	801:827	a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability	801:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	1	46	theme	bacterial	370:378	arg1	polysaccharides					329:343	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	46	theme	bacterial	370:378	arg1	cellulose					380:388	bacterial cellulose	370:388	bacterial cellulose	370:388	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	3	47	theme	double-layer	714:725	arg1	EDLCs					739:743	EDLCs	739:743	EDLCs	739:743	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	47	theme	double-layer	714:725	arg1	capacitors					727:736	electric double-layer capacitors	705:736	electric double-layer capacitors (EDLCs)	705:744	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	0	48	dep	polysaccharides	33:47	arg1	acid					106:109	citric acid	99:109	citric acid	99:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	48	dep	polysaccharides	33:47	arg1	cellulose					63:71	carboxymethyl cellulose	49:71	carboxymethyl cellulose	49:71	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	48	dep	polysaccharides	33:47	arg1	cellulose					84:92	bacterial cellulose	74:92	bacterial cellulose	74:92	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	48	dep	polysaccharides	33:47	arg1	polysaccharides					33:47	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	7	49	theme	storage	1227:1233	arg1	devices					1235:1241	low-cost and eco-friendly energy storage devices	1194:1241	low-cost and eco-friendly energy storage devices	1194:1241	These results suggest that the current material is a promising candidate for low-cost and eco-friendly energy storage devices.					
31590873	3	50	theme	capacitors	727:736	arg1	electrode					692:700	electrode	692:700	electrode of electric double-layer capacitors (EDLCs)	692:744	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	51	theme	excellent	650:658	arg1	performance					676:686	excellent electrochemical performance	650:686	excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs)	650:744	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	2	52	theme	resultant	412:420	arg1	HPC					422:424	The resultant HPC	408:424	The resultant HPC	408:424	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	3	53	theme	electric	705:712	arg1	EDLCs					739:743	EDLCs	739:743	EDLCs	739:743	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	3	53	theme	electric	705:712	arg1	capacitors					727:736	electric double-layer capacitors	705:736	electric double-layer capacitors (EDLCs)	705:744	The developed structures with macropores, mesopore walls, micropores, and high oxygen content led to excellent electrochemical performance for electrode of electric double-layer capacitors (EDLCs).					
31590873	4	54	theme	g-1	838:840	arg1	capacitance					817:827	a high specific capacitance	801:827	a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability	801:896	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	6	55	theme	light-emitting	1082:1095	arg1	diode					1097:1101	light light-emitting diode	1076:1101	light light-emitting diode (LED, 3.0 V)	1076:1114	More importantly, two series-wound devices were easy to light light-emitting diode (LED, 3.0 V).					
31590873	1	56	theme	excellent	150:158	arg1	performance					175:185	excellent supercapacitor performance	150:185	excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	150:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	2	57	theme	porous	443:448	arg1	morphology					460:469	unique porous nanosheet morphology	436:469	unique porous nanosheet morphology	436:469	The resultant HPC displayed unique porous nanosheet morphology with high specific surface area (2490 m2 g-1) and rich oxygen content (7.3%).					
31590873	6	58	theme	series-wound	1042:1053	arg1	devices					1055:1061	two series-wound devices	1038:1061	two series-wound devices	1038:1061	More importantly, two series-wound devices were easy to light light-emitting diode (LED, 3.0 V).					
31590873	0	59	theme	bacterial	74:82	arg1	cellulose					84:92	bacterial cellulose	74:92	bacterial cellulose	74:92	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	0	59	theme	bacterial	74:82	arg1	polysaccharides					33:47	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	33:109	Hierarchical porous carbons from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid for supercapacitor.					
31590873	1	60	dep	polysaccharides	329:343	arg1	polysaccharides					329:343	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	329:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	60	dep	polysaccharides	329:343	arg1	acid					402:405	citric acid	395:405	citric acid	395:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	60	dep	polysaccharides	329:343	arg1	cellulose					359:367	carboxymethyl cellulose	345:367	carboxymethyl cellulose	345:367	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	60	dep	polysaccharides	329:343	arg1	cellulose					380:388	bacterial cellulose	370:388	bacterial cellulose	370:388	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	4	61	theme	350 F	832:836	arg1	g-1					838:840	350 F g-1	832:840	350 F g-1	832:840	In a three-electrode system, the HPC electrode showed a high specific capacitance of 350 F g-1, good rate performance, and excellent cycling stability.					
31590873	1	62	theme	supercapacitor	160:173	arg1	performance					175:185	excellent supercapacitor performance	150:185	excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid	150:405	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
31590873	1	63	theme	one-step	270:277	arg1	carbonization					279:291	one-step carbonization	270:291	one-step carbonization	270:291	This study reports excellent supercapacitor performance of hierarchical composite porous carbon (HPC) materials successfully fabricated by one-step carbonization and activation process derived from polysaccharides carboxymethyl cellulose, bacterial cellulose, and citric acid.					
29729338	9	0	theme	water	1257:1261	arg1	properties					1277:1286	water vapor barrier properties	1257:1286	water vapor barrier properties of TPS matrix	1257:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	1	1	theme	polysaccharide-based	170:189	arg1	films					204:208	polysaccharide-based biocomposite films	170:208	polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch	170:313	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	11	2	theme	transmission	1474:1485	arg1	TEM					1508:1510	TEM	1508:1510	TEM	1508:1510	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	11	2	theme	transmission	1474:1485	arg1	microscopy					1496:1505	the transmission electron microscopy	1470:1505	the transmission electron microscopy (TEM)	1470:1511	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	6	3	theme	loaded	991:996	arg1	films					1002:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	7	4	theme	Tensile	1074:1080	arg1	strength					1082:1089	Tensile strength	1074:1089	Tensile strength	1074:1089	Tensile strength and elastic modulus increased by up to 80% and 170% respectively.					
29729338	9	5	theme	vapor	1263:1267	arg1	properties					1277:1286	water vapor barrier properties	1257:1286	water vapor barrier properties of TPS matrix	1257:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	1	6	theme	biocomposite	191:202	arg1	films					204:208	polysaccharide-based biocomposite films	170:208	polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch	170:313	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	4	7	theme	henequen	611:618	arg1	fibers					620:625	short henequen fibers	605:625	short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process	605:693	CNF is successfully prepared from short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process.					
29729338	9	8	theme	barrier	1269:1275	arg1	properties					1277:1286	water vapor barrier properties	1257:1286	water vapor barrier properties of TPS matrix	1257:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	5	9	theme	TPS/CNF	696:702	arg1	films					714:718	TPS/CNF composite films	696:718	TPS/CNF composite films	696:718	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	11	10	theme	electron	1487:1494	arg1	TEM					1508:1510	TEM	1508:1510	TEM	1508:1510	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	11	10	theme	electron	1487:1494	arg1	microscopy					1496:1505	the transmission electron microscopy	1470:1505	the transmission electron microscopy (TEM)	1470:1511	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	2	11	theme	starch-based	335:346	arg1	films					348:352	starch-based films	335:352	starch-based films	335:352	The application of starch-based films is limited due to highly hydrophilic nature and poor mechanical properties.					
29729338	6	12	theme	%	985:985	arg1	CNF					987:989	The 0.4 wt% CNF	975:989	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	5	13	theme	composite	704:712	arg1	films					714:718	TPS/CNF composite films	696:718	TPS/CNF composite films	696:718	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	10	14	theme	surface	1348:1354	arg1	topography					1330:1339	the topography	1326:1339	the topography of the surface of the nanocomposite	1326:1375	The AFM analysis shows the topography of the surface of the nanocomposite.					
29729338	2	15	theme	poor	402:405	arg1	properties					418:427	poor mechanical properties	402:427	poor mechanical properties	402:427	The application of starch-based films is limited due to highly hydrophilic nature and poor mechanical properties.					
29729338	6	16	theme	0.4 wt	979:984	arg1	CNF					987:989	The 0.4 wt% CNF	975:989	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	7	17	theme	elastic	1095:1101	arg1	modulus					1103:1109	elastic modulus	1095:1109	elastic modulus	1095:1109	Tensile strength and elastic modulus increased by up to 80% and 170% respectively.					
29729338	1	18	theme	films	204:208	arg1	preparation					134:144	preparation	134:144	preparation	134:144	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	1	18	theme	films	204:208	arg1	characterization					150:165	characterization	150:165	characterization	150:165	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	9	19	theme	TPS	1291:1293	arg1	matrix					1295:1300	TPS matrix	1291:1300	TPS matrix	1291:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	5	20	theme	water	817:821	arg1	WVTR					848:851	WVTR	848:851	WVTR	848:851	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	20	theme	water	817:821	arg1	rate					842:845	water vapor transmission rate	817:845	water vapor transmission rate (WVTR)	817:852	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	9	21	theme	WVTR	1216:1219	arg1	results					1229:1235	WVTR and OTR results	1216:1235	WVTR and OTR results	1216:1235	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	3	22	theme	starch	500:505	arg1	nanocomposite					469:481	a nanocomposite	467:481	a nanocomposite of thermoplastic starch (TPS)	467:511	These problems are solved by forming a nanocomposite of thermoplastic starch (TPS) as matrix and cellulose nanofiber (CNF) as reinforcement.					
29729338	3	22	theme	starch	500:505	arg1	starch					500:505	thermoplastic starch	486:505	thermoplastic starch (TPS)	486:511	These problems are solved by forming a nanocomposite of thermoplastic starch (TPS) as matrix and cellulose nanofiber (CNF) as reinforcement.					
29729338	8	23	theme	%	1169:1169	arg1	CNF					1171:1173	0.5 wt% CNF	1163:1173	0.5 wt% CNF	1163:1173	Above 0.5 wt% CNF, tensile strength starts to deteriorate.					
29729338	9	24	theme	matrix	1295:1300	arg1	properties					1277:1286	water vapor barrier properties	1257:1286	water vapor barrier properties of TPS matrix	1257:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	11	25	theme	nanofibers	1396:1405	arg1	morphology					1382:1391	The morphology	1378:1391	The morphology of nanofibers	1378:1405	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	8	26	theme	0.5 wt	1163:1168	arg1	CNF					1171:1173	0.5 wt% CNF	1163:1173	0.5 wt% CNF	1163:1173	Above 0.5 wt% CNF, tensile strength starts to deteriorate.					
29729338	1	27	theme	current	105:111	arg1	study					113:117	The current study	101:117	The current study	101:117	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	5	28	theme	oxygen	886:891	arg1	OTR					912:914	OTR	912:914	OTR	912:914	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	28	theme	oxygen	886:891	arg1	rate					906:909	oxygen transmission rate	886:909	oxygen transmission rate (OTR)	886:915	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	6	29	from	improvement	1041:1051	arg1	strength					1064:1071	tensile strength	1056:1071	tensile strength	1056:1071	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	6	30	theme	maximum	1033:1039	arg1	improvement					1041:1051	approximately the maximum improvement	1015:1051	approximately the maximum improvement in tensile strength	1015:1071	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	11	31	theme	scanning	1431:1438	arg1	SEM					1461:1463	SEM	1461:1463	SEM	1461:1463	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	11	31	theme	scanning	1431:1438	arg1	microscopy					1449:1458	the scanning electron microscopy	1427:1458	the scanning electron microscopy (SEM)	1427:1464	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	5	32	theme	atomic	855:860	arg1	microscopy					868:877	atomic force microscopy	855:877	atomic force microscopy (AFM)	855:883	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	32	theme	atomic	855:860	arg1	AFM					880:882	AFM	880:882	AFM	880:882	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	33	theme	light	937:941	arg1	transmittance					943:955	light transmittance	937:955	light transmittance	937:955	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	4	34	theme	short	605:609	arg1	fibers					620:625	short henequen fibers	605:625	short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process	605:693	CNF is successfully prepared from short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process.					
29729338	5	35	theme	polymer	740:746	arg1	method					765:770	the polymer solution casting method	736:770	the polymer solution casting method	736:770	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	2	36	theme	films	348:352	arg1	application					320:330	The application	316:330	The application of starch-based films	316:352	The application of starch-based films is limited due to highly hydrophilic nature and poor mechanical properties.					
29729338	5	37	theme	force	862:866	arg1	microscopy					868:877	atomic force microscopy	855:877	atomic force microscopy (AFM)	855:883	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	37	theme	force	862:866	arg1	AFM					880:882	AFM	880:882	AFM	880:882	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	0	38	theme	composite	49:57	arg1	films					59:63	starch-based composite films	36:63	starch-based composite films	36:63	Preparation and characterization of starch-based composite films reinforced by cellulose nanofibers.					
29729338	5	39	theme	tensile	961:967	arg1	test					969:972	tensile test	961:972	tensile test	961:972	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	40	theme	solution	748:755	arg1	method					765:770	the polymer solution casting method	736:770	the polymer solution casting method	736:770	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	4	41	theme	chemo-mechanical	670:685	arg1	process					687:693	a chemo-mechanical process	668:693	a chemo-mechanical process	668:693	CNF is successfully prepared from short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process.					
29729338	6	42	theme	tensile	1056:1062	arg1	strength					1064:1071	tensile strength	1056:1071	tensile strength	1056:1071	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	1	43	theme	cellulose	243:251	arg1	nanofiber					253:261	cellulose nanofiber	243:261	cellulose nanofiber	243:261	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	0	44	theme	starch-based	36:47	arg1	films					59:63	starch-based composite films	36:63	starch-based composite films	36:63	Preparation and characterization of starch-based composite films reinforced by cellulose nanofibers.					
29729338	5	45	theme	casting	757:763	arg1	method					765:770	the polymer solution casting method	736:770	the polymer solution casting method	736:770	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	6	46	theme	CNF	987:989	arg1	films					1002:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
29729338	1	47	theme	nanofiber	253:261	arg1	incorporation					226:238	the incorporation	222:238	the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch	222:313	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	5	48	theme	transmission	829:840	arg1	WVTR					848:851	WVTR	848:851	WVTR	848:851	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	48	theme	transmission	829:840	arg1	rate					842:845	water vapor transmission rate	817:845	water vapor transmission rate (WVTR)	817:852	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	8	49	theme	tensile	1176:1182	arg1	strength					1184:1191	tensile strength	1176:1191	tensile strength	1176:1191	Above 0.5 wt% CNF, tensile strength starts to deteriorate.					
29729338	3	50	theme	thermoplastic	486:498	arg1	TPS					508:510	TPS	508:510	TPS	508:510	These problems are solved by forming a nanocomposite of thermoplastic starch (TPS) as matrix and cellulose nanofiber (CNF) as reinforcement.					
29729338	3	50	theme	thermoplastic	486:498	arg1	starch					500:505	thermoplastic starch	486:505	thermoplastic starch (TPS)	486:511	These problems are solved by forming a nanocomposite of thermoplastic starch (TPS) as matrix and cellulose nanofiber (CNF) as reinforcement.					
29729338	10	51	theme	AFM	1307:1309	arg1	analysis					1311:1318	The AFM analysis	1303:1318	The AFM analysis	1303:1318	The AFM analysis shows the topography of the surface of the nanocomposite.					
29729338	3	52	theme	cellulose	527:535	arg1	nanofiber					537:545	cellulose nanofiber	527:545	cellulose nanofiber	527:545	These problems are solved by forming a nanocomposite of thermoplastic starch (TPS) as matrix and cellulose nanofiber (CNF) as reinforcement.					
29729338	0	53	theme	films	59:63	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of starch-based composite films reinforced by cellulose nanofibers.					
29729338	0	53	theme	films	59:63	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of starch-based composite films reinforced by cellulose nanofibers.					
29729338	9	54	theme	OTR	1225:1227	arg1	results					1229:1235	WVTR and OTR results	1216:1235	WVTR and OTR results	1216:1235	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	4	55	dep	%	653:653	arg1	60					651:652	60	651:652	60	651:652	CNF is successfully prepared from short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process.					
29729338	4	56	dep	cellulose	655:663	arg1	%					653:653	%	653:653	%	653:653	CNF is successfully prepared from short henequen fibers which consist of almost 60% cellulose by a chemo-mechanical process.					
29729338	2	57	theme	hydrophilic	379:389	arg1	nature					391:396	highly hydrophilic nature	372:396	highly hydrophilic nature	372:396	The application of starch-based films is limited due to highly hydrophilic nature and poor mechanical properties.					
29729338	10	58	theme	nanocomposite	1363:1375	arg1	surface					1348:1354	the surface	1344:1354	the surface of the nanocomposite	1344:1375	The AFM analysis shows the topography of the surface of the nanocomposite.					
29729338	5	59	theme	X-ray	918:922	arg1	diffraction					924:934	X-ray diffraction	918:934	X-ray diffraction	918:934	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	1	60	dep	preparation	134:144	arg1	the					130:132	the	130:132	the	130:132	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	11	61	theme	electron	1440:1447	arg1	SEM					1461:1463	SEM	1461:1463	SEM	1461:1463	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	11	61	theme	electron	1440:1447	arg1	microscopy					1449:1458	the scanning electron microscopy	1427:1458	the scanning electron microscopy (SEM)	1427:1464	The morphology of nanofibers is studied by using the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).					
29729338	1	62	theme	glycerol	270:277	arg1	matrix					291:296	glycerol plasticized matrix	270:296	glycerol plasticized matrix formed by starch	270:313	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	0	63	theme	cellulose	79:87	arg1	nanofibers					89:98	cellulose nanofibers	79:98	cellulose nanofibers	79:98	Preparation and characterization of starch-based composite films reinforced by cellulose nanofibers.					
29729338	5	64	theme	transmission	893:904	arg1	OTR					912:914	OTR	912:914	OTR	912:914	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	64	theme	transmission	893:904	arg1	rate					906:909	oxygen transmission rate	886:909	oxygen transmission rate (OTR)	886:915	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	1	65	theme	plasticized	279:289	arg1	matrix					291:296	glycerol plasticized matrix	270:296	glycerol plasticized matrix formed by starch	270:313	The current study deals with the preparation and characterization of polysaccharide-based biocomposite films acquired by the incorporation of cellulose nanofiber within glycerol plasticized matrix formed by starch.					
29729338	9	66	from	improvement	1242:1252	arg1	properties					1277:1286	water vapor barrier properties	1257:1286	water vapor barrier properties of TPS matrix	1257:1300	WVTR and OTR results show improvement in water vapor barrier properties of TPS matrix.					
29729338	5	67	theme	vapor	823:827	arg1	WVTR					848:851	WVTR	848:851	WVTR	848:851	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	5	67	theme	vapor	823:827	arg1	rate					842:845	water vapor transmission rate	817:845	water vapor transmission rate (WVTR)	817:852	TPS/CNF composite films are prepared by the polymer solution casting method, and their characterizations are obtained by water vapor transmission rate (WVTR), atomic force microscopy (AFM), oxygen transmission rate (OTR), X-ray diffraction, light transmittance and tensile test.					
29729338	2	68	theme	mechanical	407:416	arg1	properties					418:427	poor mechanical properties	402:427	poor mechanical properties	402:427	The application of starch-based films is limited due to highly hydrophilic nature and poor mechanical properties.					
29729338	7	69	dep	80	1130:1131	arg1	to					1127:1128	to	1127:1128	to	1127:1128	Tensile strength and elastic modulus increased by up to 80% and 170% respectively.					
29729338	6	70	theme	TPS	998:1000	arg1	films					1002:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films	975:1006	The 0.4 wt% CNF loaded TPS films showed approximately the maximum improvement in tensile strength.					
31056269	2	0	theme	immobilized	151:161	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	0	theme	immobilized	151:161	arg1	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	0	theme	immobilized	151:161	arg1	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	0	theme	immobilized	151:161	arg1	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	5	1	theme	entropy	943:949	arg1	separations					962:972	some entropy controlled separations	938:972	some entropy controlled separations	938:972	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	4	2	theme	analyte	628:634	arg1	structure					636:644	the analyte structure	624:644	the analyte structure	624:644	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	0	3	theme	stationary	96:105	arg1	phases					107:112	chiral stationary phases	89:112	chiral stationary phases	89:112	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	3	4	theme	normal	564:569	arg1	mode					577:580	normal phase mode	564:580	normal phase mode	564:580	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	6	5	located	observed	1116:1123	arg2	retention					1036:1044	an increase retention	1024:1044	an increase retention with increasing temperature accompanied with increased resolution	1024:1110	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	6	5	located	observed	1116:1123	arg1	column					1145:1150	the Chiralpak IC column	1128:1150	the Chiralpak IC column	1128:1150	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	6	5	located	observed	1116:1123	arg2	phenomenon					1012:1021	unusual phenomenon	1004:1021	unusual phenomenon	1004:1021	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	7	6	theme	enantiomers	1174:1184	arg1	order					1165:1169	The elution order	1153:1169	The elution order of enantiomers	1153:1184	The elution order of enantiomers was determined in some cases and reversed elution order was also observed.					
31056269	2	7	from	bioactive	358:366	arg1	cases					352:356	most cases	347:356	most cases	347:356	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	8	theme	derivatives	300:310	arg1	enantioseparation					276:292	enantioseparation	276:292	enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether	276:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	4	9	theme	structure	636:644	arg1	effects					587:593	The effects	583:593	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation	583:710	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	5	10	theme	ln	812:813	arg1	plots					805:809	van´t Hoff plots	794:809	van´t Hoff plots (ln k versus 1/T)	794:827	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	10	theme	ln	812:813	arg1	k					815:815	ln k versus 1/T	812:826	k	815:815	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	3	11	theme	polysaccharide	538:551	arg1	columns					553:559	two different polysaccharide columns	524:559	two different polysaccharide columns in normal phase mode	524:580	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	5	12	dep	range	848:852	arg1	to					858:859	to	858:859	to	858:859	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	2	13	from	cases	352:356	arg1	bioactive					358:366	bioactive	358:366	bioactive	358:366	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	6	14	theme	IC	1142:1143	arg1	column					1145:1150	the Chiralpak IC column	1128:1150	the Chiralpak IC column	1128:1150	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	2	15	theme	1-methoxyspirobrassinol	431:453	arg1	ether					462:466	1-methoxyspirobrassinol methyl ether	431:466	1-methoxyspirobrassinol methyl ether	431:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	16	theme	stationary	185:194	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	16	theme	stationary	185:194	arg1	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	16	theme	stationary	185:194	arg1	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	16	theme	stationary	185:194	arg1	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	3	17	dep	resolved	503:510	arg1	baseline					494:501	baseline	494:501	baseline	494:501	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	5	18	theme	most	922:925	arg1	cases					927:931	most cases	922:931	most cases	922:931	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	0	19	theme	chromatographic	7:21	arg1	recognition					30:40	Liquid chromatographic chiral recognition	0:40	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides	0:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	2	20	theme	chiral	178:183	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	20	theme	chiral	178:183	arg1	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	20	theme	chiral	178:183	arg1	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	20	theme	chiral	178:183	arg1	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	6	21	theme	increase	1027:1034	arg1	retention					1036:1044	an increase retention	1024:1044	an increase retention with increasing temperature accompanied with increased resolution	1024:1110	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	0	22	dep	phases	107:112	arg1	recognition					30:40	Liquid chromatographic chiral recognition	0:40	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides	0:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	3	23	theme	all	476:478	arg1	analytes					480:487	Almost all analytes	469:487	Almost all analytes	469:487	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	0	24	theme	Liquid	0:5	arg1	recognition					30:40	Liquid chromatographic chiral recognition	0:40	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides	0:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	4	25	theme	phase	605:609	arg1	composition					611:621	mobile phase composition	598:621	mobile phase composition	598:621	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	7	26	theme	elution	1157:1163	arg1	order					1165:1169	The elution order	1153:1169	The elution order of enantiomers	1153:1184	The elution order of enantiomers was determined in some cases and reversed elution order was also observed.					
31056269	2	27	theme	phytoalexins	330:341	arg1	derivatives					300:310	36 derivatives	297:310	36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether	297:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	5	28	theme	controlled	951:960	arg1	separations					962:972	some entropy controlled separations	938:972	some entropy controlled separations	938:972	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	7	29	theme	elution	1228:1234	arg1	order					1236:1240	reversed elution order	1219:1240	reversed elution order	1219:1240	The elution order of enantiomers was determined in some cases and reversed elution order was also observed.					
31056269	4	30	theme	mobile	598:603	arg1	composition					611:621	mobile phase composition	598:621	mobile phase composition	598:621	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	5	31	theme	parameters	777:786	arg1	Evaluation					731:740	Evaluation	731:740	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C	731:865	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	3	32	from	columns	553:559	arg1	mode					577:580	normal phase mode	564:580	normal phase mode	564:580	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	2	33	theme	indole	323:328	arg1	ether					462:466	1-methoxyspirobrassinol methyl ether	431:466	1-methoxyspirobrassinol methyl ether	431:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	33	theme	indole	323:328	arg1	phytoalexins					330:341	natural indole phytoalexins	315:341	natural indole phytoalexins	315:341	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	33	theme	indole	323:328	arg1	1-methoxyspirobrassinin					403:425	1-methoxyspirobrassinin	403:425	1-methoxyspirobrassinin	403:425	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	33	theme	indole	323:328	arg1	spirobrassinin					387:400	racemic spirobrassinin	379:400	racemic spirobrassinin	379:400	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	1	34	theme	Unusual	115:121	arg1	behavior					135:142	Unusual temperature behavior	115:142	Unusual temperature behavior.	115:143	Unusual temperature behavior.					
31056269	2	35	theme	Chiralpak	218:226	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	35	theme	Chiralpak	218:226	arg1	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	36	theme	natural	315:321	arg1	ether					462:466	1-methoxyspirobrassinol methyl ether	431:466	1-methoxyspirobrassinol methyl ether	431:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	36	theme	natural	315:321	arg1	phytoalexins					330:341	natural indole phytoalexins	315:341	natural indole phytoalexins	315:341	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	36	theme	natural	315:321	arg1	1-methoxyspirobrassinin					403:425	1-methoxyspirobrassinin	403:425	1-methoxyspirobrassinin	403:425	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	36	theme	natural	315:321	arg1	spirobrassinin					387:400	racemic spirobrassinin	379:400	racemic spirobrassinin	379:400	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	37	used	used	254:257	arg2	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	37	used	used	254:257	arg2	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	37	used	used	254:257	arg2	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	37	used	used	254:257	arg2	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	0	38	theme	phytoalexins	45:56	arg1	recognition					30:40	Liquid chromatographic chiral recognition	0:40	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides	0:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	4	39	theme	composition	611:621	arg1	effects					587:593	The effects	583:593	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation	583:710	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	5	40	theme	van´t	794:798	arg1	1/T					824:826	ln k versus 1/T	812:826	1/T	824:826	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	40	theme	van´t	794:798	arg1	plots					805:809	van´t Hoff plots	794:809	van´t Hoff plots (ln k versus 1/T)	794:827	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	40	theme	van´t	794:798	arg1	k					815:815	ln k versus 1/T	812:826	k	815:815	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	6	41	theme	Chiralpak	1132:1140	arg1	column					1145:1150	the Chiralpak IC column	1128:1150	the Chiralpak IC column	1128:1150	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	4	42	from	effects	587:593	arg1	enantioseparation					694:710	enantioseparation	694:710	enantioseparation	694:710	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	4	42	from	effects	587:593	arg1	retention					680:688	retention	680:688	retention	680:688	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	6	43	theme	unusual	1004:1010	arg1	phenomenon					1012:1021	unusual phenomenon	1004:1021	unusual phenomenon	1004:1021	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	5	44	theme	temperature	836:846	arg1	range					848:852	the temperature range -15 to 50 °C	832:865	the temperature range -15 to 50 °C	832:865	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	2	45	theme	methyl	455:460	arg1	ether					462:466	1-methoxyspirobrassinol methyl ether	431:466	1-methoxyspirobrassinol methyl ether	431:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	0	46	theme	immobilized	61:71	arg1	polysaccharides					73:87	immobilized polysaccharides	61:87	immobilized polysaccharides	61:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	2	47	theme	Chiralpak	204:212	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	47	theme	Chiralpak	204:212	arg1	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	4	48	dep	retention	680:688	arg1	the					676:678	the	676:678	the	676:678	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	5	49	from	controlled	908:917	arg1	cases					927:931	most cases	922:931	most cases	922:931	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	2	50	theme	bioactive	358:366	arg1	ether					462:466	1-methoxyspirobrassinol methyl ether	431:466	1-methoxyspirobrassinol methyl ether	431:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	50	theme	bioactive	358:366	arg1	phytoalexins					330:341	natural indole phytoalexins	315:341	natural indole phytoalexins	315:341	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	50	theme	bioactive	358:366	arg1	1-methoxyspirobrassinin					403:425	1-methoxyspirobrassinin	403:425	1-methoxyspirobrassinin	403:425	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	50	theme	bioactive	358:366	arg1	spirobrassinin					387:400	racemic spirobrassinin	379:400	racemic spirobrassinin	379:400	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	5	51	theme	Hoff	800:803	arg1	1/T					824:826	ln k versus 1/T	812:826	1/T	824:826	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	51	theme	Hoff	800:803	arg1	plots					805:809	van´t Hoff plots	794:809	van´t Hoff plots (ln k versus 1/T)	794:827	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	51	theme	Hoff	800:803	arg1	k					815:815	ln k versus 1/T	812:826	k	815:815	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	2	52	theme	polysaccharide	163:176	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	52	theme	polysaccharide	163:176	arg1	IA					214:215	Chiralpak IA	204:215	Chiralpak IA	204:215	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	52	theme	polysaccharide	163:176	arg1	IB					228:229	Chiralpak IB	218:229	Chiralpak IB	218:229	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	52	theme	polysaccharide	163:176	arg1	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	5	53	theme	corresponding	749:761	arg1	parameters					777:786	the corresponding thermodynamic parameters	745:786	the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C	745:865	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	2	54	theme	Chiralpak	235:243	arg1	phases					196:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases	145:201	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	54	theme	Chiralpak	235:243	arg1	IC					245:246	Chiralpak IC	235:246	Chiralpak IC	235:246	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	55	theme	enantioseparation	276:292	arg1	study					267:271	the study	263:271	the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether	263:466	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	2	56	theme	most	347:350	arg1	cases					352:356	most cases	347:356	most cases	347:356	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	1	57	theme	temperature	123:133	arg1	behavior					135:142	Unusual temperature behavior	115:142	Unusual temperature behavior.	115:143	Unusual temperature behavior.					
31056269	4	58	theme	column	654:659	arg1	temperature					661:671	the column temperature	650:671	the column temperature	650:671	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31056269	5	59	theme	thermodynamic	763:775	arg1	parameters					777:786	the corresponding thermodynamic parameters	745:786	the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C	745:865	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	5	60	from	cases	927:931	arg1	controlled					908:917	controlled	908:917	controlled	908:917	Evaluation of the corresponding thermodynamic parameters using van´t Hoff plots (ln k versus 1/T) in the temperature range -15 to 50 °C indicated that separations were enthalpy controlled in most cases, but some entropy controlled separations were also observed.					
31056269	3	61	theme	different	528:536	arg1	columns					553:559	two different polysaccharide columns	524:559	two different polysaccharide columns in normal phase mode	524:580	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	6	62	theme	increased	1091:1099	arg1	resolution					1101:1110	increased resolution	1091:1110	increased resolution	1091:1110	Moreover, unusual phenomenon, an increase retention with increasing temperature accompanied with increased resolution was observed on the Chiralpak IC column.					
31056269	0	63	theme	chiral	89:94	arg1	phases					107:112	chiral stationary phases	89:112	chiral stationary phases	89:112	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	3	64	theme	phase	571:575	arg1	mode					577:580	normal phase mode	564:580	normal phase mode	564:580	Almost all analytes were baseline resolved at least on two different polysaccharide columns in normal phase mode.					
31056269	7	65	theme	reversed	1219:1226	arg1	order					1236:1240	reversed elution order	1219:1240	reversed elution order	1219:1240	The elution order of enantiomers was determined in some cases and reversed elution order was also observed.					
31056269	0	66	from	recognition	30:40	arg1	polysaccharides					73:87	immobilized polysaccharides	61:87	immobilized polysaccharides	61:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	0	67	theme	chiral	23:28	arg1	recognition					30:40	Liquid chromatographic chiral recognition	0:40	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides	0:87	Liquid chromatographic chiral recognition of phytoalexins on immobilized polysaccharides chiral stationary phases.					
31056269	2	68	theme	racemic	379:385	arg1	spirobrassinin					387:400	racemic spirobrassinin	379:400	racemic spirobrassinin	379:400	Three immobilized polysaccharide chiral stationary phases, Chiralpak IA, Chiralpak IB and Chiralpak IC, were used for the study of enantioseparation of 36 derivatives of natural indole phytoalexins, in most cases bioactive, including racemic spirobrassinin, 1-methoxyspirobrassinin and 1-methoxyspirobrassinol methyl ether.					
31056269	4	69	theme	temperature	661:671	arg1	effects					587:593	The effects	583:593	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation	583:710	The effects of mobile phase composition, the analyte structure and the column temperature on the retention and enantioseparation were investigated.					
31353707	8	0	theme	1.23 mg/ml	1163:1172	arg1	values					1144:1149	IC50 values	1139:1149	IC50 values	1139:1149	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	3	1	dep	separation	271:280	arg1	column					316:321	DEAE-52 cellulose anion-exchange column	283:321	DEAE-52 cellulose anion-exchange column	283:321	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	3	1	dep	separation	271:280	arg1	column					346:351	Sephadex G-100 gel column	327:351	Sephadex G-100 gel column	327:351	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	6	2	theme	hexahedron	818:827	arg1	structure					829:837	a straight hexahedron structure	807:837	a straight hexahedron structure	807:837	Meanwhile, DIP-1 presented a straight hexahedron structure, but no triple-helical conformation.					
31353707	7	3	theme	antioxidant	879:889	arg1	tests					900:904	antioxidant activity tests	879:904	antioxidant activity tests	879:904	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	3	4	theme	water	211:215	arg1	extraction					217:226	hot water extraction	207:226	hot water extraction	207:226	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	4	5	theme	DIP-1	389:393	arg1	properties					375:384	The physicochemical properties	355:384	The physicochemical properties of DIP-1	355:393	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	13	6	theme	present	1812:1818	arg1	study					1820:1824	the present study	1808:1824	the present study	1808:1824	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	2	7	theme	neutral	124:130	arg1	Andr					178:181	Andr	178:181	Andr	178:181	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	2	7	theme	neutral	124:130	arg1	DIP-1					148:152	DIP-1	148:152	DIP-1	148:152	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	2	7	theme	neutral	124:130	arg1	polysaccharide					132:145	A neutral polysaccharide	122:145	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)	122:183	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	12	8	theme	significant	1747:1757	arg1	activities					1785:1794	significant antioxidant and antitumor activities	1747:1794	significant antioxidant and antitumor activities	1747:1794	Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities.					
31353707	4	9	theme	monosaccharide	445:458	arg1	composition					460:470	monosaccharide composition	445:470	monosaccharide composition	445:470	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	3	10	theme	chromatographic	255:269	arg1	separation					271:280	chromatographic separation	255:280	chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column)	255:352	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	12	11	theme	neutral	1715:1721	arg1	polysaccharide					1723:1736	the neutral polysaccharide	1711:1736	the neutral polysaccharide	1711:1736	Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities.					
31353707	4	12	theme	gel	414:416	arg1	chromatography					429:442	gel permeation chromatography	414:442	gel permeation chromatography	414:442	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	3	13	theme	cellulose	291:299	arg1	column					316:321	DEAE-52 cellulose anion-exchange column	283:321	DEAE-52 cellulose anion-exchange column	283:321	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	0	14	from	antioxidant	18:28	arg1	indica					100:105	Duchesnea indica	90:105	Duchesnea indica	90:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	13	15	theme	functional	1907:1916	arg1	industries					1942:1951	functional food and pharmaceutical industries	1907:1951	functional food and pharmaceutical industries	1907:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	0	16	theme	polysaccharide	70:83	arg1	antioxidant					18:28	antioxidant	18:28	antioxidant	18:28	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	16	theme	polysaccharide	70:83	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	16	theme	polysaccharide	70:83	arg1	activities					46:55	anticancer activities	35:55	anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)	35:113	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	16	theme	polysaccharide	70:83	arg1	Andr					108:111	Andr	108:111	Andr	108:111	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	9	17	theme	many	1290:1293	arg1	diseases					1295:1302	many diseases	1290:1302	many diseases	1290:1302	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	9	17	theme	many	1290:1293	arg1	cancer					1315:1320	cancer	1315:1320	cancer	1315:1320	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	0	18	from	indica	100:105	arg1	polysaccharide					70:83	a neutral polysaccharide	60:83	a neutral polysaccharide from Duchesnea indica	60:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	18	from	indica	100:105	arg1	antioxidant					18:28	antioxidant	18:28	antioxidant	18:28	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	18	from	indica	100:105	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	18	from	indica	100:105	arg1	activities					46:55	anticancer activities	35:55	anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)	35:113	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	18	from	indica	100:105	arg1	Andr					108:111	Andr	108:111	Andr	108:111	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	3	19	theme	gel	342:344	arg1	column					346:351	Sephadex G-100 gel column	327:351	Sephadex G-100 gel column	327:351	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	0	20	from	Characterization	0:15	arg1	indica					100:105	Duchesnea indica	90:105	Duchesnea indica	90:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	10	21	theme	comprehensive	1416:1428	arg1	attention					1430:1438	comprehensive attention	1416:1438	comprehensive attention	1416:1438	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	9	22	theme	D.	1213:1214	arg1	indica					1216:1221	D. indica	1213:1221	D. indica	1213:1221	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	9	22	theme	D.	1213:1214	arg1	medicine					1266:1273	a Chinese medicine	1256:1273	a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1256:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	0	23	theme	Duchesnea	90:98	arg1	indica					100:105	Duchesnea indica	90:105	Duchesnea indica	90:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	8	24	theme	high	1065:1068	arg1	activities					1081:1090	high inhibitory activities	1065:1090	high inhibitory activities against SKOV-3 and Hep-G2 cells	1065:1122	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	7	25	theme	ABTS	973:976	arg1	radicals					978:985	ABTS radicals	973:985	ABTS radicals	973:985	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	3	26	theme	Sephadex	327:334	arg1	column					346:351	Sephadex G-100 gel column	327:351	Sephadex G-100 gel column	327:351	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	0	27	from	activities	46:55	arg1	indica					100:105	Duchesnea indica	90:105	Duchesnea indica	90:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	13	28	theme	D.	1878:1879	arg1	polysaccharides					1888:1902	D. indica polysaccharides	1878:1902	D. indica polysaccharides	1878:1902	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	13	29	theme	polysaccharides	1888:1902	arg1	application					1863:1873	the high-value application	1848:1873	the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries	1848:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	8	30	theme	SKOV-3	1100:1105	arg1	cells					1118:1122	SKOV-3 and Hep-G2 cells	1100:1122	SKOV-3 and Hep-G2 cells	1100:1122	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	11	31	from	indica	1674:1679	arg1	polysaccharide					1651:1664	a neutral polysaccharide	1641:1664	a neutral polysaccharide from D. indica	1641:1679	In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time.					
31353707	10	32	theme	biological	1488:1497	arg1	properties					1499:1508	pharmacological and biological properties	1468:1508	pharmacological and biological properties	1468:1508	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	7	33	from	activities	943:952	arg1	DPPH					967:970	DPPH	967:970	DPPH	967:970	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	7	33	from	activities	943:952	arg1	radicals					978:985	ABTS radicals	973:985	ABTS radicals	973:985	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	7	33	from	activities	943:952	arg1	power					1000:1004	reducing power	991:1004	reducing power	991:1004	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	7	33	from	activities	943:952	arg1	hydroxyl					957:964	hydroxyl	957:964	hydroxyl	957:964	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	4	34	dep	Fourier	473:479	arg1	transform					481:489	transform	481:489	transform infrared spectrometry	481:511	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	8	35	theme	Hep-G2	1111:1116	arg1	cells					1118:1122	SKOV-3 and Hep-G2 cells	1100:1122	SKOV-3 and Hep-G2 cells	1100:1122	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	4	36	theme	red	583:585	arg1	test					587:590	Congo red test	577:590	Congo red test	577:590	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	10	37	theme	pharmacological	1468:1482	arg1	properties					1499:1508	pharmacological and biological properties	1468:1508	pharmacological and biological properties	1468:1508	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	13	38	from	application	1863:1873	arg1	industries					1942:1951	functional food and pharmaceutical industries	1907:1951	functional food and pharmaceutical industries	1907:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	11	39	theme	first	1689:1693	arg1	time					1695:1698	the first time	1685:1698	the first time	1685:1698	In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time.					
31353707	4	40	theme	electron	553:560	arg1	microscope					562:571	scanning electron microscope	544:571	scanning electron microscope	544:571	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	8	41	theme	IC50	1139:1142	arg1	values					1144:1149	IC50 values	1139:1149	IC50 values	1139:1149	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	7	42	theme	scavenging	932:941	arg1	activities					943:952	powerful scavenging activities	923:952	powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power	923:1004	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	9	43	theme	PRACTICAL	1189:1197	arg1	APPLICATIONS					1199:1210	PRACTICAL APPLICATIONS	1189:1210	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.	1189:1371	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	12	44	theme	antitumor	1775:1783	arg1	activities					1785:1794	significant antioxidant and antitumor activities	1747:1794	significant antioxidant and antitumor activities	1747:1794	Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities.					
31353707	2	45	from	indica	170:175	arg1	Andr					178:181	Andr	178:181	Andr	178:181	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	2	45	from	indica	170:175	arg1	DIP-1					148:152	DIP-1	148:152	DIP-1	148:152	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	2	45	from	indica	170:175	arg1	polysaccharide					132:145	A neutral polysaccharide	122:145	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)	122:183	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	10	46	theme	immunomodulatory	1562:1577	arg1	activity					1579:1586	immunomodulatory activity	1562:1586	immunomodulatory activity	1562:1586	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	6	47	theme	straight	809:816	arg1	structure					829:837	a straight hexahedron structure	807:837	a straight hexahedron structure	807:837	Meanwhile, DIP-1 presented a straight hexahedron structure, but no triple-helical conformation.					
31353707	7	48	theme	activity	891:898	arg1	tests					900:904	antioxidant activity tests	879:904	antioxidant activity tests	879:904	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	11	49	theme	D.	1671:1672	arg1	indica					1674:1679	D. indica	1671:1679	D. indica	1671:1679	In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time.					
31353707	3	50	theme	hot	207:209	arg1	extraction					217:226	hot water extraction	207:226	hot water extraction	207:226	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	4	51	theme	physicochemical	359:373	arg1	properties					375:384	The physicochemical properties	355:384	The physicochemical properties of DIP-1	355:393	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	12	52	theme	antioxidant	1759:1769	arg1	activities					1785:1794	significant antioxidant and antitumor activities	1747:1794	significant antioxidant and antitumor activities	1747:1794	Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities.					
31353707	5	53	theme	1.00:0.42:18.36:14.17:0.81	712:737	arg1	ratio					703:707	a ratio	701:707	a ratio of 1.00:0.42:18.36:14.17:0.81	701:737	The results indicated that DIP-1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa.					
31353707	9	54	theme	long	1243:1246	arg1	time					1248:1251	a long time	1241:1251	a long time	1241:1251	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	3	55	theme	ethanol	229:235	arg1	precipitation					237:249	ethanol precipitation	229:249	ethanol precipitation	229:249	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	4	56	theme	UV-visible	514:523	arg1	spectrophotometry					525:541	UV-visible spectrophotometry	514:541	UV-visible spectrophotometry	514:541	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	3	57	theme	DEAE-52	283:289	arg1	cellulose					291:299	cellulose	291:299	cellulose	291:299	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	13	58	theme	pharmaceutical	1927:1940	arg1	industries					1942:1951	functional food and pharmaceutical industries	1907:1951	functional food and pharmaceutical industries	1907:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	9	59	theme	Chinese	1258:1264	arg1	indica					1216:1221	D. indica	1213:1221	D. indica	1213:1221	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	9	59	theme	Chinese	1258:1264	arg1	medicine					1266:1273	a Chinese medicine	1256:1273	a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1256:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	4	60	theme	permeation	418:427	arg1	chromatography					429:442	gel permeation chromatography	414:442	gel permeation chromatography	414:442	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	9	61	dep	APPLICATIONS	1199:1210	arg1	used					1232:1235	used	1232:1235	has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1223:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	0	62	theme	neutral	62:68	arg1	polysaccharide					70:83	a neutral polysaccharide	60:83	a neutral polysaccharide from Duchesnea indica	60:105	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	7	63	theme	dose-dependent	1011:1024	arg1	manner					1026:1031	a dose-dependent manner	1009:1031	a dose-dependent manner	1009:1031	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	3	64	theme	anion-exchange	301:314	arg1	column					316:321	DEAE-52 cellulose anion-exchange column	283:321	DEAE-52 cellulose anion-exchange column	283:321	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	13	65	theme	food	1918:1921	arg1	industries					1942:1951	functional food and pharmaceutical industries	1907:1951	functional food and pharmaceutical industries	1907:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	9	66	theme	diseases	1295:1302	arg1	therapy					1279:1285	therapy	1279:1285	therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1279:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	8	67	theme	inhibitory	1070:1079	arg1	activities					1081:1090	high inhibitory activities	1065:1090	high inhibitory activities against SKOV-3 and Hep-G2 cells	1065:1122	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	7	68	theme	reducing	991:998	arg1	power					1000:1004	reducing power	991:1004	reducing power	991:1004	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	9	69	used	used	1232:1235	arg2	medicine					1266:1273	a Chinese medicine	1256:1273	a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1256:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	9	69	used	used	1232:1235	arg2	indica					1216:1221	D. indica	1213:1221	D. indica	1213:1221	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	5	70	theme	molecular	748:756	arg1	weight					758:763	its molecular weight	744:763	its molecular weight	744:763	The results indicated that DIP-1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa.					
31353707	3	71	theme	G-100	336:340	arg1	column					346:351	Sephadex G-100 gel column	327:351	Sephadex G-100 gel column	327:351	Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column).					
31353707	10	72	theme	large	1453:1457	arg1	antioxidant					1546:1556	antioxidant	1546:1556	antioxidant	1546:1556	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	72	theme	large	1453:1457	arg1	antitumor					1521:1529	antitumor	1521:1529	antitumor	1521:1529	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	72	theme	large	1453:1457	arg1	activity					1579:1586	immunomodulatory activity	1562:1586	immunomodulatory activity	1562:1586	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	72	theme	large	1453:1457	arg1	antidiabetic					1532:1543	antidiabetic	1532:1543	antidiabetic	1532:1543	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	72	theme	large	1453:1457	arg1	range					1459:1463	a large range	1451:1463	a large range	1451:1463	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	13	73	theme	indica	1881:1886	arg1	polysaccharides					1888:1902	D. indica polysaccharides	1878:1902	D. indica polysaccharides	1878:1902	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	9	74	dep	and	1362:1364	arg1	so					1366:1367	so	1366:1367	so	1366:1367	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	4	75	theme	Congo	577:581	arg1	test					587:590	Congo red test	577:590	Congo red test	577:590	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	4	76	dep	transform	481:489	arg1	infrared					491:498	infrared	491:498	transform infrared spectrometry	481:511	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	2	77	theme	Duchesnea	160:168	arg1	indica					170:175	Duchesnea indica	160:175	Duchesnea indica	160:175	A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.)					
31353707	13	78	theme	high-value	1852:1861	arg1	application					1863:1873	the high-value application	1848:1873	the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries	1848:1951	Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.					
31353707	0	79	theme	anticancer	35:44	arg1	activities					46:55	anticancer activities	35:55	anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)	35:113	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	0	79	theme	anticancer	35:44	arg1	Andr					108:111	Andr	108:111	Andr	108:111	Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.)					
31353707	4	80	theme	scanning	544:551	arg1	microscope					562:571	scanning electron microscope	544:571	scanning electron microscope	544:571	The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test.					
31353707	11	81	theme	present	1596:1602	arg1	study					1604:1608	the present study	1592:1608	the present study	1592:1608	In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time.					
31353707	6	82	theme	triple-helical	847:860	arg1	conformation					862:873	triple-helical conformation	847:873	triple-helical conformation	847:873	Meanwhile, DIP-1 presented a straight hexahedron structure, but no triple-helical conformation.					
31353707	10	83	theme	properties	1499:1508	arg1	antioxidant					1546:1556	antioxidant	1546:1556	antioxidant	1546:1556	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	83	theme	properties	1499:1508	arg1	antitumor					1521:1529	antitumor	1521:1529	antitumor	1521:1529	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	83	theme	properties	1499:1508	arg1	activity					1579:1586	immunomodulatory activity	1562:1586	immunomodulatory activity	1562:1586	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	83	theme	properties	1499:1508	arg1	antidiabetic					1532:1543	antidiabetic	1532:1543	antidiabetic	1532:1543	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	10	83	theme	properties	1499:1508	arg1	range					1459:1463	a large range	1451:1463	a large range	1451:1463	At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity.					
31353707	7	84	theme	powerful	923:930	arg1	activities					943:952	powerful scavenging activities	923:952	powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power	923:1004	In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner.					
31353707	9	85	theme	on	1369:1370	arg1	therapy					1279:1285	therapy	1279:1285	therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on	1279:1370	PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on.					
31353707	8	86	theme	1.42	1154:1157	arg1	values					1144:1149	IC50 values	1139:1149	IC50 values	1139:1149	Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively.					
31353707	11	87	theme	neutral	1643:1649	arg1	polysaccharide					1651:1664	a neutral polysaccharide	1641:1664	a neutral polysaccharide from D. indica	1641:1679	In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time.					
31187991	5	0	theme	turbidity	788:796	arg1	formation					775:783	the formation	771:783	the formation of turbidity and protection mechanisms	771:822	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	6	1	theme	polyphenol	1020:1029	arg1	source					1031:1036	polyphenol source	1020:1036	polyphenol source	1020:1036	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	1	theme	polyphenol	1020:1029	arg1	extract					1000:1006	a commercial grape tannin extract	974:1006	a commercial grape tannin extract	974:1006	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	0	2	from	Interactions	34:45	arg1	Presence					54:61	the Presence	50:61	the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems	50:121	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	5	3	theme	Small-scale	673:683	arg1	trials					691:696	Small-scale bench trials	673:696	Small-scale bench trials	673:696	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	2	4	theme	colloidal	381:389	arg1	solutions					391:399	colloidal solutions	381:399	colloidal solutions	381:399	Different polysaccharides can influence these interactions by protecting or disrupting charges and are even used as additives to stabilize colloidal solutions.					
31187991	4	5	theme	unexpected	593:602	arg1	problems					604:611	unexpected problems	593:611	unexpected problems	593:611	In some cases, the mechanisms that are involved in these reactions are not thoroughly understood and can lead to unexpected problems and delayed haze formation after CMC addition to red wines.					
31187991	0	6	theme	Model	109:113	arg1	Systems					115:121	Wine-Like Model Systems	99:121	Wine-Like Model Systems	99:121	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	5	7	theme	bench	685:689	arg1	trials					691:696	Small-scale bench trials	673:696	Small-scale bench trials	673:696	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	0	8	theme	Wine-Like	99:107	arg1	Systems					115:121	Wine-Like Model Systems	99:121	Wine-Like Model Systems	99:121	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	6	9	theme	protein	928:934	arg1	protein					904:910	Egg-white protein	894:910	Egg-white protein	894:910	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	9	theme	protein	928:934	arg1	model					936:940	a protein model	926:940	a protein model due to its complex composition	926:971	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	9	10	theme	Other	1371:1375	arg1	polysaccharides					1377:1391	Other polysaccharides	1371:1391	Other polysaccharides	1371:1391	Other polysaccharides delayed the reaction between proteins and other macromolecules depending on their concentration.					
31187991	6	11	theme	various	1137:1143	arg1	conditions					1150:1159	various wine conditions	1137:1159	various wine conditions	1137:1159	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	1	12	theme	wine	186:189	arg1	assessment					201:210	wine stability assessment	186:210	wine stability assessment	186:210	Protein-polyphenol interactions play a very important role in wine stability assessment, especially in red varieties.					
31187991	5	13	theme	protection	802:811	arg1	formation					775:783	the formation	771:783	the formation of turbidity and protection mechanisms	771:822	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	10	14	theme	experiments	1511:1521	arg1	results					1494:1500	The results	1490:1500	The results of these experiments	1490:1521	The results of these experiments provide important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine.					
31187991	1	15	theme	stability	191:199	arg1	assessment					201:210	wine stability assessment	186:210	wine stability assessment	186:210	Protein-polyphenol interactions play a very important role in wine stability assessment, especially in red varieties.					
31187991	0	16	from	Presence	54:61	arg1	Systems					115:121	Wine-Like Model Systems	99:121	Wine-Like Model Systems	99:121	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	10	17	theme	physical	1621:1628	arg1	stability					1630:1638	the physical stability	1617:1638	the physical stability of wine	1617:1646	The results of these experiments provide important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine.					
31187991	10	18	theme	wine	1643:1646	arg1	stability					1630:1638	the physical stability	1617:1638	the physical stability of wine	1617:1646	The results of these experiments provide important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine.					
31187991	6	19	used	used	1012:1015	arg2	extract					1000:1006	a commercial grape tannin extract	974:1006	a commercial grape tannin extract	974:1006	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	19	used	used	1012:1015	arg2	source					1031:1036	polyphenol source	1020:1036	polyphenol source	1020:1036	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	3	20	theme	most	406:409	arg1	examples					418:425	The most common examples	402:425	The most common examples	402:425	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	3	20	theme	most	406:409	arg1	mannoproteins					431:443	mannoproteins	431:443	mannoproteins	431:443	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	6	21	theme	tannin	993:998	arg1	source					1031:1036	polyphenol source	1020:1036	polyphenol source	1020:1036	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	21	theme	tannin	993:998	arg1	extract					1000:1006	a commercial grape tannin extract	974:1006	a commercial grape tannin extract	974:1006	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	5	22	dep	turbidity	788:796	arg1	mechanisms					813:822	mechanisms	813:822	mechanisms	813:822	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	5	23	theme	polyphenols	860:870	arg1	interaction					835:845	the interaction	831:845	the interaction of proteins, polyphenols, and polysaccharides	831:891	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	3	24	theme	common	411:416	arg1	examples					418:425	The most common examples	402:425	The most common examples	402:425	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	3	24	theme	common	411:416	arg1	mannoproteins					431:443	mannoproteins	431:443	mannoproteins	431:443	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	6	25	theme	grape	987:991	arg1	source					1031:1036	polyphenol source	1020:1036	polyphenol source	1020:1036	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	25	theme	grape	987:991	arg1	extract					1000:1006	a commercial grape tannin extract	974:1006	a commercial grape tannin extract	974:1006	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	10	26	theme	important	1531:1539	arg1	insights					1541:1548	important insights	1531:1548	important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine	1531:1646	The results of these experiments provide important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine.					
31187991	6	27	theme	wine	1145:1148	arg1	conditions					1150:1159	various wine conditions	1137:1159	various wine conditions	1137:1159	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	3	28	theme	carboxymethyl	449:461	arg1	CMC					474:476	CMC	474:476	CMC	474:476	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	3	28	theme	carboxymethyl	449:461	arg1	cellulose					463:471	carboxymethyl cellulose	449:471	carboxymethyl cellulose (CMC)	449:477	The most common examples are mannoproteins and carboxymethyl cellulose (CMC).					
31187991	6	29	theme	commercial	976:985	arg1	source					1031:1036	polyphenol source	1020:1036	polyphenol source	1020:1036	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	29	theme	commercial	976:985	arg1	extract					1000:1006	a commercial grape tannin extract	974:1006	a commercial grape tannin extract	974:1006	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	5	30	theme	different	736:744	arg1	conditions					749:758	different pH conditions	736:758	different pH conditions	736:758	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	1	31	theme	red	227:229	arg1	varieties					231:239	red varieties	227:239	red varieties	227:239	Protein-polyphenol interactions play a very important role in wine stability assessment, especially in red varieties.					
31187991	5	32	theme	pH	746:747	arg1	conditions					749:758	different pH conditions	736:758	different pH conditions	736:758	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	6	33	theme	Egg-white	894:902	arg1	protein					904:910	Egg-white protein	894:910	Egg-white protein	894:910	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	6	33	theme	Egg-white	894:902	arg1	model					936:940	a protein model	926:940	a protein model due to its complex composition	926:971	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	5	34	theme	proteins	850:857	arg1	interaction					835:845	the interaction	831:845	the interaction of proteins, polyphenols, and polysaccharides	831:891	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	4	35	theme	red	662:664	arg1	wines					666:670	red wines	662:670	red wines	662:670	In some cases, the mechanisms that are involved in these reactions are not thoroughly understood and can lead to unexpected problems and delayed haze formation after CMC addition to red wines.					
31187991	0	36	theme	Cellulose	80:88	arg1	Presence					54:61	the Presence	50:61	the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems	50:121	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	7	37	theme	50	1205:1206	arg1	mL					1208:1209	mL	1208:1209	mL	1208:1209	Reactions were monitored in duplicate on a 50 mL scale by spectrophotometry at 860 nm over at least 30 days.					
31187991	2	38	theme	Different	242:250	arg1	polysaccharides					252:266	Different polysaccharides	242:266	Different polysaccharides	242:266	Different polysaccharides can influence these interactions by protecting or disrupting charges and are even used as additives to stabilize colloidal solutions.					
31187991	2	38	theme	Different	242:250	arg1	additives					358:366	additives	358:366	additives	358:366	Different polysaccharides can influence these interactions by protecting or disrupting charges and are even used as additives to stabilize colloidal solutions.					
31187991	1	39	theme	Protein-polyphenol	124:141	arg1	interactions					143:154	Protein-polyphenol interactions	124:154	Protein-polyphenol interactions	124:154	Protein-polyphenol interactions play a very important role in wine stability assessment, especially in red varieties.					
31187991	0	40	theme	Carboxymethyl	66:78	arg1	CMC					91:93	CMC	91:93	CMC	91:93	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	0	40	theme	Carboxymethyl	66:78	arg1	Cellulose					80:88	Carboxymethyl Cellulose	66:88	Carboxymethyl Cellulose (CMC)	66:94	Polyphenol-Protein-Polysaccharide Interactions in the Presence of Carboxymethyl Cellulose (CMC) in Wine-Like Model Systems.					
31187991	6	41	theme	complex	953:959	arg1	composition					961:971	its complex composition	949:971	its complex composition	949:971	Egg-white protein was chosen as a protein model due to its complex composition, a commercial grape tannin extract was used as polyphenol source, and pectin, glucomannan, mannoprotein, alginate, and CMC were applied as polysaccharides to model various wine conditions.					
31187991	10	42	theme	reaction	1555:1562	arg1	dynamics					1564:1571	reaction dynamics	1555:1571	reaction dynamics between macromolecules that are involved in the physical stability of wine	1555:1646	The results of these experiments provide important insights into reaction dynamics between macromolecules that are involved in the physical stability of wine.					
31187991	5	43	theme	model	716:720	arg1	systems					722:728	model systems	716:728	model systems under different pH conditions	716:758	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	9	44	theme	other	1435:1439	arg1	macromolecules					1441:1454	other macromolecules	1435:1454	other macromolecules	1435:1454	Other polysaccharides delayed the reaction between proteins and other macromolecules depending on their concentration.					
31187991	4	45	theme	haze	625:628	arg1	formation					630:638	haze formation	625:638	haze formation	625:638	In some cases, the mechanisms that are involved in these reactions are not thoroughly understood and can lead to unexpected problems and delayed haze formation after CMC addition to red wines.					
31187991	5	46	theme	polysaccharides	877:891	arg1	interaction					835:845	the interaction	831:845	the interaction of proteins, polyphenols, and polysaccharides	831:891	Small-scale bench trials were conducted in model systems under different pH conditions to monitor the formation of turbidity and protection mechanisms during the interaction of proteins, polyphenols, and polysaccharides.					
31187991	2	47	used	used	350:353	arg2	additives					358:366	additives	358:366	additives	358:366	Different polysaccharides can influence these interactions by protecting or disrupting charges and are even used as additives to stabilize colloidal solutions.					
31187991	2	47	used	used	350:353	arg2	polysaccharides					252:266	Different polysaccharides	242:266	Different polysaccharides	242:266	Different polysaccharides can influence these interactions by protecting or disrupting charges and are even used as additives to stabilize colloidal solutions.					
31187991	4	48	theme	CMC	646:648	arg1	addition					650:657	CMC addition	646:657	CMC addition to red wines	646:670	In some cases, the mechanisms that are involved in these reactions are not thoroughly understood and can lead to unexpected problems and delayed haze formation after CMC addition to red wines.					
31187991	1	49	theme	important	168:176	arg1	role					178:181	a very important role	161:181	a very important role	161:181	Protein-polyphenol interactions play a very important role in wine stability assessment, especially in red varieties.					
31187991	7	50	theme	mL	1208:1209	arg1	scale					1211:1215	a 50 mL scale	1203:1215	a 50 mL scale by spectrophotometry	1203:1236	Reactions were monitored in duplicate on a 50 mL scale by spectrophotometry at 860 nm over at least 30 days.					
30560450	4	0	contain	had	731:733	arg2	abundance					746:754	the lowest abundance	735:754	the lowest abundance of F. oxysporum	735:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	0	contain	had	731:733	arg2	pH					776:777	pH	776:777	pH	776:777	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	0	contain	had	731:733	arg1	soil					708:711	The rhizosphere soil	692:711	The rhizosphere soil of healthy plants	692:729	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	8	1	theme	wilt	1474:1477	arg1	expression					1479:1488	Fusarium wilt expression	1465:1488	Fusarium wilt expression	1465:1488	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	6	2	from	differences	956:966	arg1	compositions					986:997	soil microbial compositions	971:997	soil microbial compositions	971:997	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	0	3	from	differences	12:22	arg1	characteristics					54:68	the chemical and microbial characteristics	27:68	the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils	27:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	1	4	theme	comprehensive	172:184	arg1	effect					186:191	the comprehensive effect	168:191	the comprehensive effect of soil physicochemical and biological properties	168:241	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	4	5	theme	oxysporum	762:770	arg1	pH					776:777	pH	776:777	pH	776:777	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	5	theme	oxysporum	762:770	arg1	abundance					746:754	the lowest abundance	735:754	the lowest abundance of F. oxysporum	735:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	5	theme	oxysporum	762:770	arg1	contents					795:802	the highest contents	783:802	the highest contents of ammonium (NH4+) and nitrate (NO3-)	783:840	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	0	6	theme	watermelon	108:117	arg1	soils					131:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	3	7	theme	watermelon	538:547	arg1	plants					549:554	watermelon plants	538:554	watermelon plants grown in a greenhouse under the same field management practices	538:618	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	8	8	theme	Fusarium	1465:1472	arg1	expression					1479:1488	Fusarium wilt expression	1465:1488	Fusarium wilt expression	1465:1488	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	0	9	theme	wilt-infected	94:106	arg1	soils					131:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	4	10	theme	healthy	716:722	arg1	plants					724:729	healthy plants	716:729	healthy plants	716:729	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	8	11	theme	chemical	1367:1374	arg1	properties					1376:1385	some chemical properties	1362:1385	some chemical properties of the rhizosphere soil of healthy plants	1362:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	7	12	contain	had	1193:1195	arg1	microbiome					1140:1149	The microbiome	1136:1149	The microbiome in the rhizosphere soil of healthy plants	1136:1191	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	7	12	contain	had	1193:1195	arg2	abundances					1278:1287	the lowest relative abundances	1258:1287	the lowest relative abundances of potential pathogens	1258:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	7	12	contain	had	1193:1195	arg2	abundances					1218:1227	the highest relative abundances	1197:1227	the highest relative abundances of potential antagonists	1197:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	3	13	theme	rhizosphere	500:510	arg1	soils					512:516	The rhizosphere soils	496:516	The rhizosphere soils	496:516	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	0	14	theme	soils	131:135	arg1	characteristics					54:68	the chemical and microbial characteristics	27:68	the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils	27:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	4	15	theme	lowest	739:744	arg1	abundance					746:754	the lowest abundance	735:754	the lowest abundance of F. oxysporum	735:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	2	16	theme	potential	434:442	arg1	roles					444:448	their potential roles	428:448	their potential roles in plant health and Fusarium wilt expression	428:493	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	5	17	theme	relative	847:854	arg1	content					856:862	The relative content	843:862	The relative content of hemicellulose	843:879	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	0	18	theme	rhizosphere	119:129	arg1	soils					131:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	4	19	theme	highest	787:793	arg1	contents					795:802	the highest contents	783:802	the highest contents of ammonium (NH4+) and nitrate (NO3-)	783:840	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	7	20	theme	healthy	1178:1184	arg1	plants					1186:1191	healthy plants	1178:1191	healthy plants	1178:1191	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	6	21	from	statuses	1041:1048	arg1	watermelons					1009:1019	the watermelons	1005:1019	the watermelons at the three health statuses	1005:1048	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	3	22	theme	same	588:591	arg1	practices					610:618	the same field management practices	584:618	the same field management practices	584:618	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	1	23	theme	soil	196:199	arg1	properties					232:241	soil physicochemical and biological properties	196:241	soil physicochemical and biological properties	196:241	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	7	24	theme	plants	1186:1191	arg1	soil					1170:1173	the rhizosphere soil	1154:1173	the rhizosphere soil of healthy plants	1154:1191	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	6	25	theme	microbial	976:984	arg1	compositions					986:997	soil microbial compositions	971:997	soil microbial compositions	971:997	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	2	26	theme	soils	330:334	arg1	microbiomes					299:309	microbiomes	299:309	microbiomes	299:309	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	2	26	theme	soils	330:334	arg1	properties					284:293	properties	284:293	properties	284:293	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	7	27	theme	antagonists	1242:1252	arg1	abundances					1278:1287	the lowest relative abundances	1258:1287	the lowest relative abundances of potential pathogens	1258:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	7	27	theme	antagonists	1242:1252	arg1	abundances					1218:1227	the highest relative abundances	1197:1227	the highest relative abundances of potential antagonists	1197:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	2	28	theme	plants	397:402	arg1	soils					330:334	the rhizosphere soils	314:334	the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants	314:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	2	29	theme	plant	453:457	arg1	health					459:464	plant health	453:464	plant health	453:464	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	4	30	theme	F.	759:760	arg1	oxysporum					762:770	F. oxysporum	759:770	F. oxysporum	759:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	5	31	theme	F.	922:923	arg1	plants					944:949	F. oxysporum-infected plants	922:949	F. oxysporum-infected plants	922:949	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	2	32	theme	rhizosphere	318:328	arg1	soils					330:334	the rhizosphere soils	314:334	the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants	314:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	3	33	theme	field	593:597	arg1	practices					610:618	the same field management practices	584:618	the same field management practices	584:618	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	5	34	theme	plants	944:949	arg1	soil					914:917	the rhizosphere soil	898:917	the rhizosphere soil of F. oxysporum-infected plants	898:949	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	4	35	theme	plants	724:729	arg1	soil					708:711	The rhizosphere soil	692:711	The rhizosphere soil of healthy plants	692:729	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	1	36	theme	biological	221:230	arg1	properties					232:241	soil physicochemical and biological properties	196:241	soil physicochemical and biological properties	196:241	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	7	37	theme	pathogens	1302:1310	arg1	abundances					1278:1287	the lowest relative abundances	1258:1287	the lowest relative abundances of potential pathogens	1258:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	7	37	theme	pathogens	1302:1310	arg1	abundances					1218:1227	the highest relative abundances	1197:1227	the highest relative abundances of potential antagonists	1197:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	7	38	theme	lowest	1262:1267	arg1	abundances					1278:1287	the lowest relative abundances	1258:1287	the lowest relative abundances of potential pathogens	1258:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	1	39	theme	physicochemical	201:215	arg1	properties					232:241	soil physicochemical and biological properties	196:241	soil physicochemical and biological properties	196:241	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	1	40	theme	properties	232:241	arg1	effect					186:191	the comprehensive effect	168:191	the comprehensive effect of soil physicochemical and biological properties	168:241	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	0	41	theme	chemical	31:38	arg1	characteristics					54:68	the chemical and microbial characteristics	27:68	the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils	27:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	7	42	theme	rhizosphere	1158:1168	arg1	soil					1170:1173	the rhizosphere soil	1154:1173	the rhizosphere soil of healthy plants	1154:1191	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	3	43	theme	chemical	652:659	arg1	characteristics					661:675	various soil microbial and chemical characteristics	625:675	various soil microbial and chemical characteristics	625:675	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	8	44	theme	plants	1422:1427	arg1	soil					1406:1409	the rhizosphere soil	1390:1409	the rhizosphere soil of healthy plants	1390:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	8	45	theme	specific	1317:1324	arg1	responsible					1438:1448	responsible	1438:1448	responsible	1438:1448	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	8	45	theme	specific	1317:1324	arg1	composition					1336:1346	The specific microbial composition	1313:1346	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants	1313:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	2	46	theme	wilt	479:482	arg1	expression					484:493	Fusarium wilt expression	470:493	Fusarium wilt expression	470:493	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	7	47	theme	highest	1201:1207	arg1	abundances					1218:1227	the highest relative abundances	1197:1227	the highest relative abundances of potential antagonists	1197:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	8	48	theme	soil	1406:1409	arg1	properties					1376:1385	some chemical properties	1362:1385	some chemical properties of the rhizosphere soil of healthy plants	1362:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	6	49	theme	soil	971:974	arg1	compositions					986:997	soil microbial compositions	971:997	soil microbial compositions	971:997	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	3	50	theme	management	599:608	arg1	practices					610:618	the same field management practices	584:618	the same field management practices	584:618	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	6	51	theme	plant	1115:1119	arg1	status					1128:1133	plant health status	1115:1133	plant health status	1115:1133	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	2	52	theme	oxysporum-infected	357:374	arg1	plants					397:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	0	53	theme	microbial	44:52	arg1	characteristics					54:68	the chemical and microbial characteristics	27:68	the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils	27:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	7	54	theme	potential	1232:1240	arg1	antagonists					1242:1252	potential antagonists	1232:1252	potential antagonists	1232:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	2	55	theme	Fusarium	470:477	arg1	expression					484:493	Fusarium wilt expression	470:493	Fusarium wilt expression	470:493	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	4	56	theme	nitrate	827:833	arg1	pH					776:777	pH	776:777	pH	776:777	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	56	theme	nitrate	827:833	arg1	abundance					746:754	the lowest abundance	735:754	the lowest abundance of F. oxysporum	735:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	56	theme	nitrate	827:833	arg1	contents					795:802	the highest contents	783:802	the highest contents of ammonium (NH4+) and nitrate (NO3-)	783:840	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	7	57	theme	potential	1292:1300	arg1	pathogens					1302:1310	potential pathogens	1292:1310	potential pathogens	1292:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	1	58	theme	Plant	138:142	arg1	health					144:149	Plant health	138:149	Plant health	138:149	Plant health is determined by the comprehensive effect of soil physicochemical and biological properties.					
30560450	7	59	theme	relative	1269:1276	arg1	abundances					1278:1287	the lowest relative abundances	1258:1287	the lowest relative abundances of potential pathogens	1258:1310	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	5	60	theme	oxysporum-infected	925:942	arg1	plants					944:949	F. oxysporum-infected plants	922:949	F. oxysporum-infected plants	922:949	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	3	61	theme	microbial	638:646	arg1	characteristics					661:675	various soil microbial and chemical characteristics	625:675	various soil microbial and chemical characteristics	625:675	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	2	62	theme	chemical	275:282	arg1	properties					284:293	properties	284:293	properties	284:293	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	8	63	theme	microbial	1326:1334	arg1	responsible					1438:1448	responsible	1438:1448	responsible	1438:1448	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	8	63	theme	microbial	1326:1334	arg1	composition					1336:1346	The specific microbial composition	1313:1346	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants	1313:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	5	64	theme	hemicellulose	867:879	arg1	content					856:862	The relative content	843:862	The relative content of hemicellulose	843:879	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	6	65	theme	health	1034:1039	arg1	statuses					1041:1048	the three health statuses	1024:1048	the three health statuses	1024:1048	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	6	66	theme	health	1121:1126	arg1	status					1128:1133	plant health status	1115:1133	plant health status	1115:1133	The differences in soil microbial compositions among the watermelons at the three health statuses were obvious, and their microbiomes changed gradually along with plant health status.					
30560450	5	67	theme	rhizosphere	902:912	arg1	soil					914:917	the rhizosphere soil	898:917	the rhizosphere soil of F. oxysporum-infected plants	898:949	The relative content of hemicellulose was decreased in the rhizosphere soil of F. oxysporum-infected plants.					
30560450	2	68	theme	healthy	339:345	arg1	plants					397:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	8	69	theme	rhizosphere	1394:1404	arg1	soil					1406:1409	the rhizosphere soil	1390:1409	the rhizosphere soil of healthy plants	1390:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	4	70	theme	ammonium	807:814	arg1	pH					776:777	pH	776:777	pH	776:777	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	70	theme	ammonium	807:814	arg1	abundance					746:754	the lowest abundance	735:754	the lowest abundance of F. oxysporum	735:770	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	4	70	theme	ammonium	807:814	arg1	contents					795:802	the highest contents	783:802	the highest contents of ammonium (NH4+) and nitrate (NO3-)	783:840	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	0	71	theme	healthy	73:79	arg1	soils					131:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	healthy and Fusarium wilt-infected watermelon rhizosphere soils	73:135	Deciphering differences in the chemical and microbial characteristics of healthy and Fusarium wilt-infected watermelon rhizosphere soils.					
30560450	8	72	theme	healthy	1414:1420	arg1	plants					1422:1427	healthy plants	1414:1427	healthy plants	1414:1427	The specific microbial composition together with some chemical properties of the rhizosphere soil of healthy plants might be responsible for inhibiting Fusarium wilt expression.					
30560450	3	73	theme	various	625:631	arg1	characteristics					661:675	various soil microbial and chemical characteristics	625:675	various soil microbial and chemical characteristics	625:675	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	2	74	theme	watermelon	386:395	arg1	plants					397:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	2	75	dep	properties	284:293	arg1	the					271:273	the	271:273	the	271:273	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	4	76	theme	rhizosphere	696:706	arg1	soil					708:711	The rhizosphere soil	692:711	The rhizosphere soil of healthy plants	692:729	The rhizosphere soil of healthy plants had the lowest abundance of F. oxysporum and pH and the highest contents of ammonium (NH4+) and nitrate (NO3-).					
30560450	7	77	from	microbiome	1140:1149	arg1	soil					1170:1173	the rhizosphere soil	1154:1173	the rhizosphere soil of healthy plants	1154:1191	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30560450	3	78	theme	soil	633:636	arg1	characteristics					661:675	various soil microbial and chemical characteristics	625:675	various soil microbial and chemical characteristics	625:675	The rhizosphere soils were collected from watermelon plants grown in a greenhouse under the same field management practices, and various soil microbial and chemical characteristics were analyzed.					
30560450	2	79	from	roles	444:448	arg1	health					459:464	plant health	453:464	plant health	453:464	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	2	79	from	roles	444:448	arg1	expression					484:493	Fusarium wilt expression	470:493	Fusarium wilt expression	470:493	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	2	80	theme	dead	381:384	arg1	plants					397:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	healthy, Fusarium oxysporum-infected, and dead watermelon plants	339:402	In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plant health and Fusarium wilt expression.					
30560450	7	81	theme	relative	1209:1216	arg1	abundances					1218:1227	the highest relative abundances	1197:1227	the highest relative abundances of potential antagonists	1197:1252	The microbiome in the rhizosphere soil of healthy plants had the highest relative abundances of potential antagonists and the lowest relative abundances of potential pathogens.					
30409574	2	0	theme	yellow	422:427	arg1	OSYPF					440:444	OSYPF	440:444	OSYPF	440:444	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	0	theme	yellow	422:427	arg1	flour					433:437	organic split yellow pea flour	408:437	organic split yellow pea flour (OSYPF)	408:445	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	4	1	theme	%	567:567	arg1	starch					569:574	78-91% starch	562:574	78-91% starch	562:574	A range of 78-91% starch was enriched in the coarse fractions.					
30409574	1	2	theme	milling	162:168	arg1	influence					132:140	influence	132:140	influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions	132:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	2	3	theme	split	416:420	arg1	OSYPF					440:444	OSYPF	440:444	OSYPF	440:444	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	3	theme	split	416:420	arg1	flour					433:437	organic split yellow pea flour	408:437	organic split yellow pea flour (OSYPF)	408:445	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	5	4	theme	proximate	647:655	arg1	analysis					657:664	proximate analysis	647:664	proximate analysis	647:664	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	5	5	dep	fine	708:711	arg1	fractions					724:732	fractions	724:732	fractions	724:732	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	5	5	dep	fine	708:711	arg1	the					704:706	the	704:706	the	704:706	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	4	6	theme	78-91	562:566	arg1	%					567:567	%	567:567	%	567:567	A range of 78-91% starch was enriched in the coarse fractions.					
30409574	6	7	theme	size	748:751	arg1	analysis					753:760	The particle size analysis	735:760	The particle size analysis	735:760	The particle size analysis demonstrated that the milling in this study was sufficient and effective in reducing the flour particle size to smaller than 22 µm, which was considered to be the cut-point.					
30409574	0	8	theme	dry	91:93	arg1	fractionation					95:107	dry fractionation	91:107	dry fractionation	91:107	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	3	9	theme	85-87	462:466	arg1	%					467:467	%	467:467	%	467:467	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	6	10	from	milling	784:790	arg1	study					800:804	this study	795:804	this study	795:804	The particle size analysis demonstrated that the milling in this study was sufficient and effective in reducing the flour particle size to smaller than 22 µm, which was considered to be the cut-point.					
30409574	7	11	theme	fine	953:956	arg1	fraction					958:965	the fine fraction	949:965	the fine fraction	949:965	The yield of the fine fraction was as high as 44% and acceptable for an industrial manufacture.					
30409574	2	12	theme	split	374:378	arg1	SGPF					397:400	SGPF	397:400	SGPF	397:400	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	12	theme	split	374:378	arg1	flour					390:394	split green pea flour	374:394	split green pea flour (SGPF)	374:401	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	1	13	theme	air	174:176	arg1	classification					178:191	industrial scale milling and air classification	145:191	classification	178:191	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	5	14	theme	compositional	676:688	arg1	shift					690:694	compositional shift	676:694	compositional shift between the fine and coarse fractions	676:732	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	2	15	theme	organic	408:414	arg1	OSYPF					440:444	OSYPF	440:444	OSYPF	440:444	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	15	theme	organic	408:414	arg1	flour					433:437	organic split yellow pea flour	408:437	organic split yellow pea flour (OSYPF)	408:445	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	1	16	from	influence	132:140	arg1	separation					196:205	separation	196:205	separation of pea flours into fine and coarse fractions	196:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	2	17	theme	pea	276:278	arg1	flours					280:285	Three commercial field pea flours	253:285	Three commercial field pea flours	253:285	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	1	18	theme	classification	178:191	arg1	influence					132:140	influence	132:140	influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions	132:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	0	19	theme	scale	11:15	arg1	preparation					17:27	Industrial scale preparation	0:27	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.	0:108	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	5	20	theme	Scanning	614:621	arg1	microscopy					632:641	Scanning electron microscopy	614:641	Scanning electron microscopy	614:641	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	4	21	theme	coarse	596:601	arg1	fractions					603:611	the coarse fractions	592:611	the coarse fractions	592:611	A range of 78-91% starch was enriched in the coarse fractions.					
30409574	7	22	theme	industrial	1008:1017	arg1	manufacture					1019:1029	an industrial manufacture	1005:1029	an industrial manufacture	1005:1029	The yield of the fine fraction was as high as 44% and acceptable for an industrial manufacture.					
30409574	0	23	theme	Industrial	0:9	arg1	preparation					17:27	Industrial scale preparation	0:27	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.	0:108	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	6	24	theme	particle	857:864	arg1	size					866:869	the flour particle size	847:869	the flour particle size	847:869	The particle size analysis demonstrated that the milling in this study was sufficient and effective in reducing the flour particle size to smaller than 22 µm, which was considered to be the cut-point.					
30409574	3	25	theme	74-95	478:482	arg1	%					483:483	%	483:483	%	483:483	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	2	26	theme	pea	356:358	arg1	WGPF					367:370	WGPF	367:370	WGPF	367:370	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	26	theme	pea	356:358	arg1	flour					360:364	whole green pea flour	344:364	whole green pea flour (WGPF)	344:371	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	6	27	theme	flour	851:855	arg1	size					866:869	the flour particle size	847:869	the flour particle size	847:869	The particle size analysis demonstrated that the milling in this study was sufficient and effective in reducing the flour particle size to smaller than 22 µm, which was considered to be the cut-point.					
30409574	2	28	theme	field	304:308	arg1	peas					310:313	field peas	304:313	field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF)	304:445	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	7	29	theme	fraction	958:965	arg1	high					974:977	high	974:977	high	974:977	The yield of the fine fraction was as high as 44% and acceptable for an industrial manufacture.					
30409574	7	29	theme	fraction	958:965	arg1	yield					940:944	The yield	936:944	The yield of the fine fraction	936:965	The yield of the fine fraction was as high as 44% and acceptable for an industrial manufacture.					
30409574	2	30	theme	green	380:384	arg1	SGPF					397:400	SGPF	397:400	SGPF	397:400	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	30	theme	green	380:384	arg1	flour					390:394	split green pea flour	374:394	split green pea flour (SGPF)	374:401	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	31	theme	pea	386:388	arg1	SGPF					397:400	SGPF	397:400	SGPF	397:400	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	31	theme	pea	386:388	arg1	flour					390:394	split green pea flour	374:394	split green pea flour (SGPF)	374:401	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	32	theme	green	350:354	arg1	WGPF					367:370	WGPF	367:370	WGPF	367:370	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	32	theme	green	350:354	arg1	flour					360:364	whole green pea flour	344:364	whole green pea flour (WGPF)	344:371	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	3	33	theme	protein	469:475	arg1	ranges					452:457	The ranges	448:457	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals	448:508	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	1	34	theme	pea	210:212	arg1	flours					214:219	pea flours	210:219	pea flours	210:219	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	0	35	theme	flour	36:40	arg1	fractions					42:50	pea flour fractions	32:50	pea flour fractions	32:50	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	3	36	theme	pea	531:533	arg1	fractions					540:548	the pea fine fractions	527:548	the pea fine fractions	527:548	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	1	37	theme	flours	214:219	arg1	separation					196:205	separation	196:205	separation of pea flours into fine and coarse fractions	196:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	0	38	theme	pea	32:34	arg1	fractions					42:50	pea flour fractions	32:50	pea flour fractions	32:50	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	6	39	theme	particle	739:746	arg1	analysis					753:760	The particle size analysis	735:760	The particle size analysis	735:760	The particle size analysis demonstrated that the milling in this study was sufficient and effective in reducing the flour particle size to smaller than 22 µm, which was considered to be the cut-point.					
30409574	2	40	theme	pea	429:431	arg1	OSYPF					440:444	OSYPF	440:444	OSYPF	440:444	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	40	theme	pea	429:431	arg1	flour					433:437	organic split yellow pea flour	408:437	organic split yellow pea flour (OSYPF)	408:445	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	3	41	theme	%	483:483	arg1	fat					485:487	74-95% fat	478:487	74-95% fat	478:487	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	0	42	with	preparation	17:27	arg1	composition					76:86	enhanced nutritive composition	57:86	enhanced nutritive composition by dry fractionation	57:107	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	0	43	theme	fractions	42:50	arg1	preparation					17:27	Industrial scale preparation	0:27	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.	0:108	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	3	44	theme	fat	485:487	arg1	ranges					452:457	The ranges	448:457	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals	448:508	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	4	45	theme	starch	569:574	arg1	range					553:557	A range	551:557	A range of 78-91% starch	551:574	A range of 78-91% starch was enriched in the coarse fractions.					
30409574	3	46	theme	minerals	501:508	arg1	ranges					452:457	The ranges	448:457	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals	448:508	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	2	47	theme	field	270:274	arg1	flours					280:285	Three commercial field pea flours	253:285	Three commercial field pea flours	253:285	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	5	48	theme	electron	623:630	arg1	microscopy					632:641	Scanning electron microscopy	614:641	Scanning electron microscopy	614:641	Scanning electron microscopy and proximate analysis confirmed compositional shift between the fine and coarse fractions.					
30409574	3	49	theme	%	467:467	arg1	protein					469:475	85-87% protein	462:475	85-87% protein	462:475	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	1	50	theme	fine	226:229	arg1	fractions					242:250	fine and coarse fractions	226:250	fine and coarse fractions	226:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	3	51	theme	fine	535:538	arg1	fractions					540:548	the pea fine fractions	527:548	the pea fine fractions	527:548	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	2	52	theme	commercial	259:268	arg1	flours					280:285	Three commercial field pea flours	253:285	Three commercial field pea flours	253:285	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	53	theme	whole	344:348	arg1	WGPF					367:370	WGPF	367:370	WGPF	367:370	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	2	53	theme	whole	344:348	arg1	flour					360:364	whole green pea flour	344:364	whole green pea flour (WGPF)	344:371	Three commercial field pea flours were chosen from field peas grown in Manitoba, Canada, - whole green pea flour (WGPF), split green pea flour (SGPF), and organic split yellow pea flour (OSYPF).					
30409574	0	54	theme	nutritive	66:74	arg1	composition					76:86	enhanced nutritive composition	57:86	enhanced nutritive composition by dry fractionation	57:107	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	3	55	theme	66-76	494:498	arg1	%					499:499	%	499:499	%	499:499	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	1	56	theme	industrial	145:154	arg1	milling					162:168	industrial scale milling and air classification	145:191	milling	162:168	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	1	57	theme	coarse	235:240	arg1	fractions					242:250	fine and coarse fractions	226:250	fine and coarse fractions	226:250	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30409574	0	58	theme	enhanced	57:64	arg1	composition					76:86	enhanced nutritive composition	57:86	enhanced nutritive composition by dry fractionation	57:107	Industrial scale preparation of pea flour fractions with enhanced nutritive composition by dry fractionation.					
30409574	3	59	theme	%	499:499	arg1	minerals					501:508	66-76% minerals	494:508	66-76% minerals	494:508	The ranges of 85-87% protein, 74-95% fat, and 66-76% minerals were enriched in the pea fine fractions.					
30409574	1	60	theme	scale	156:160	arg1	milling					162:168	industrial scale milling and air classification	145:191	milling	162:168	This study determined influence of industrial scale milling and air classification on separation of pea flours into fine and coarse fractions.					
30567018	11	0	theme	B	1771:1771	arg1	groups					1786:1791	B, C, D, and E groups	1771:1791	B, C, D, and E groups	1771:1791	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	2	1	theme	PMP	621:623	arg1	method					651:656	PMP pre-column derivatization method	621:656	PMP pre-column derivatization method	621:656	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	5	2	theme	E	1059:1059	arg1	groups					1061:1066	C, D, and E groups	1049:1066	C, D, and E groups	1049:1066	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	2	3	theme	derivatization	636:649	arg1	method					651:656	PMP pre-column derivatization method	621:656	PMP pre-column derivatization method	621:656	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	4	4	theme	japonicum	974:982	arg1	cercariae					958:966	the cercariae	954:966	the cercariae of S. japonicum (30 ± 2 for each mouse)	954:1006	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	10	5	theme	=	1642:1642	arg1	F					1640:1640	F	1640:1640	F = 99.788	1640:1649	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	11	6	theme	D	1777:1777	arg1	groups					1786:1791	B, C, D, and E groups	1771:1791	B, C, D, and E groups	1771:1791	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	3	7	theme	normal	748:753	arg1	groups：A					738:745	five groups：A	733:745	five groups：A (normal group)	733:760	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	3	7	theme	normal	748:753	arg1	group					755:759	normal group	748:759	normal group	748:759	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	11	8	theme	C	1774:1774	arg1	groups					1786:1791	B, C, D, and E groups	1771:1791	B, C, D, and E groups	1771:1791	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	13	9	theme	S.	2033:2034	arg1	infection					2046:2054	S. japonicum infection	2033:2054	S. japonicum infection	2033:2054	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	3	10	theme	experimental	766:777	arg1	B					763:763	B	763:763	B (experimental group)	763:784	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	3	10	theme	experimental	766:777	arg1	group					779:783	experimental group	766:783	experimental group	766:783	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	13	11	theme	praziquantel	2107:2118	arg1	administration					2089:2102	the administration	2085:2102	the administration of praziquantel	2085:2118	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	12	12	from	degree	1833:1838	arg1	group					1845:1849	E group	1843:1849	E group	1843:1849	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	4	13	theme	B	888:888	arg1	groups					902:907	B, C, D, or E groups	888:907	B, C, D, or E groups	888:907	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	5	14	from	mice	1134:1137	arg1	group					1144:1148	B group	1142:1148	B group	1142:1148	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	9	15	dep	=	1532:1532	arg1	63.525					1534:1539	63.525	1534:1539	63.525	1534:1539	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	11	16	theme	HE	1693:1694	arg1	staining					1696:1703	HE staining	1693:1703	HE staining	1693:1703	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	4	17	theme	C	891:891	arg1	groups					902:907	B, C, D, or E groups	888:907	B, C, D, or E groups	888:907	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	13	18	dep	CONCLUSIONS	1909:1919	arg1	has					1961:1963	has	1961:1963	has	1961:1963	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	1	19	theme	polysaccharide	227:240	arg1	characterization					207:222	the molecular characterization	193:222	the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection	193:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	6	20	dep	livers	1182:1187	arg1	All					1174:1176	All	1174:1176	All	1174:1176	All the livers and sera were collected after 16 weeks.					
30567018	6	20	dep	livers	1182:1187	arg1	the					1178:1180	the	1178:1180	the	1178:1180	All the livers and sera were collected after 16 weeks.					
30567018	9	21	from	levels	1462:1467	arg1	groups					1493:1498	C, D, and E groups	1481:1498	C, D, and E groups	1481:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	9	22	theme	E	1491:1491	arg1	groups					1493:1498	C, D, and E groups	1481:1498	C, D, and E groups	1481:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	0	23	theme	Schistosoma	149:159	arg1	japonicum					161:169	Schistosoma japonicum	149:169	Schistosoma japonicum	149:169	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	4	24	from	mice	880:883	arg1	groups					902:907	B, C, D, or E groups	888:907	B, C, D, or E groups	888:907	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	9	25	theme	serum	1456:1460	arg1	levels					1462:1467	the serum levels	1452:1467	the serum levels of IFN-γ in C, D, and E groups	1452:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	12	26	theme	=	1889:1889	arg1	group					1879:1883	B group	1877:1883	B group (χ2 = 7.875, P < 0.05)	1877:1906	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	12	26	theme	=	1889:1889	arg1	χ2					1886:1887	χ2 = 7.875	1886:1895	χ2 = 7.875	1886:1895	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	10	27	theme	serum	1566:1570	arg1	levels					1572:1577	the serum levels	1562:1577	the serum levels of IL-13 in C, D, and E groups	1562:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	2	28	theme	monosaccharide	523:536	arg1	composition					538:548	monosaccharide composition	523:548	monosaccharide composition	523:548	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	0	29	from	intervention	94:105	arg1	detection					32:40	molecular detection	22:40	molecular detection of polysaccharide from Amusium pleuronectes	22:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	3	30	theme	polysaccharide	790:803	arg1	C					787:787	C	787:787	C (polysaccharide group)	787:810	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	3	30	theme	polysaccharide	790:803	arg1	group					805:809	polysaccharide group	790:809	polysaccharide group	790:809	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	0	31	theme	Amusium	65:71	arg1	pleuronectes					73:84	Amusium pleuronectes	65:84	Amusium pleuronectes	65:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	9	32	theme	D	1484:1484	arg1	groups					1493:1498	C, D, and E groups	1481:1498	C, D, and E groups	1481:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	12	33	theme	B	1877:1877	arg1	group					1879:1883	B group	1877:1883	B group (χ2 = 7.875, P < 0.05)	1877:1906	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	12	33	theme	B	1877:1877	arg1	χ2					1886:1887	χ2 = 7.875	1886:1895	χ2 = 7.875	1886:1895	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	11	34	theme	hepatic	1737:1743	arg1	fibrosis					1745:1752	hepatic fibrosis	1737:1752	hepatic fibrosis	1737:1752	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	1	35	theme	fibrosis	352:359	arg1	formation					331:339	the formation	327:339	the formation of hepatic fibrosis caused by Schistosoma japonicum infection	327:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	11	36	located	observed	1759:1766	arg1	groups					1786:1791	B, C, D, and E groups	1771:1791	B, C, D, and E groups	1771:1791	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	11	36	located	observed	1759:1766	arg2	fibrosis					1745:1752	hepatic fibrosis	1737:1752	hepatic fibrosis	1737:1752	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	11	36	located	observed	1759:1766	arg2	nodules					1725:1731	the egg nodules	1717:1731	the egg nodules	1717:1731	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	3	37	theme	polysaccharide	838:851	arg1	E					835:835	E	835:835	E (polysaccharide + praziquantel group)	835:873	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	3	37	theme	polysaccharide	838:851	arg1	group					868:872	polysaccharide + praziquantel group	838:872	group	868:872	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	10	38	theme	C	1591:1591	arg1	groups					1603:1608	C, D, and E groups	1591:1608	C, D, and E groups	1591:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	4	39	theme	D	894:894	arg1	groups					902:907	B, C, D, or E groups	888:907	B, C, D, or E groups	888:907	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	12	40	theme	E	1843:1843	arg1	group					1845:1849	E group	1843:1849	E group	1843:1849	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	13	41	theme	fibrosis	2005:2012	arg1	process					2014:2020	hepatic fibrosis process	1997:2020	hepatic fibrosis process induced by S. japonicum infection	1997:2054	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	9	42	theme	P	1542:1542	arg1	<					1544:1544	P < 0.01	1542:1549	P < 0.01	1542:1549	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	10	43	dep	F	1640:1640	arg1	<					1654:1654	P < 0.01	1652:1659	P < 0.01	1652:1659	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	2	44	theme	crude	416:420	arg1	polysaccharide					422:435	The crude polysaccharide	412:435	METHODS The crude polysaccharide from A. pleuronectes	404:456	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	0	45	theme	hepatic	110:116	arg1	fibrosis					118:125	hepatic fibrosis	110:125	hepatic fibrosis in rats infected with Schistosoma japonicum	110:169	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	1	46	theme	japonicum	383:391	arg1	infection					393:401	Schistosoma japonicum infection	371:401	Schistosoma japonicum infection	371:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	8	47	theme	molecular	1343:1351	arg1	kDa					1417:1419	11.7 kDa	1412:1419	11.7 kDa	1412:1419	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	8	47	theme	molecular	1343:1351	arg1	weight					1353:1358	The molecular weight	1339:1358	The molecular weight of purified polysaccharide from A. pleuronectes	1339:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	0	48	theme	[Preliminary	0:11	arg1	study					13:17	[Preliminary study	0:17	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes	0:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	5	49	theme	C	1049:1049	arg1	groups					1061:1066	C, D, and E groups	1049:1066	C, D, and E groups	1049:1066	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	10	50	dep	decreased	1629:1637	arg1	F					1640:1640	F	1640:1640	F = 99.788	1640:1649	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	5	51	theme	D	1052:1052	arg1	groups					1061:1066	C, D, and E groups	1049:1066	C, D, and E groups	1049:1066	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	10	52	from	levels	1572:1577	arg1	groups					1603:1608	C, D, and E groups	1591:1608	C, D, and E groups	1591:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	8	53	theme	polysaccharide	1372:1385	arg1	kDa					1417:1419	11.7 kDa	1412:1419	11.7 kDa	1412:1419	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	8	53	theme	polysaccharide	1372:1385	arg1	weight					1353:1358	The molecular weight	1339:1358	The molecular weight of purified polysaccharide from A. pleuronectes	1339:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	3	54	theme	BALB/c	694:699	arg1	mice					701:704	50 female BALB/c mice	684:704	50 female BALB/c mice	684:704	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	13	55	from	pleuronectes	1948:1959	arg1	polysaccharide					1925:1938	The polysaccharide	1921:1938	The polysaccharide from A. pleuronectes	1921:1959	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	1	56	theme	intervention	311:322	arg1	role					303:306	its role	299:306	its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection	299:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	8	57	theme	A.	1392:1393	arg1	pleuronectes					1395:1406	A. pleuronectes	1392:1406	A. pleuronectes	1392:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	12	58	theme	egg	1808:1810	arg1	nodules					1812:1818	egg nodules	1808:1818	egg nodules	1808:1818	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	0	59	theme	polysaccharide	45:58	arg1	detection					32:40	molecular detection	22:40	molecular detection of polysaccharide from Amusium pleuronectes	22:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	2	60	dep	METHODS	404:410	arg1	polysaccharide					422:435	The crude polysaccharide	412:435	METHODS The crude polysaccharide from A. pleuronectes	404:456	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	3	61	theme	praziquantel	855:866	arg1	E					835:835	E	835:835	E (polysaccharide + praziquantel group)	835:873	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	3	61	theme	praziquantel	855:866	arg1	group					868:872	polysaccharide + praziquantel group	838:872	group	868:872	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	1	62	theme	Amusium	256:262	arg1	pleuronectes					264:275	Amusium pleuronectes	256:275	Amusium pleuronectes	256:275	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	13	63	contain	has	1961:1963	arg1	polysaccharide					1925:1938	The polysaccharide	1921:1938	The polysaccharide from A. pleuronectes	1921:1959	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	13	63	contain	has	1961:1963	arg2	effect					1976:1981	an obvious effect	1965:1981	an obvious effect	1965:1981	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	2	64	from	pleuronectes	445:456	arg1	polysaccharide					422:435	The crude polysaccharide	412:435	METHODS The crude polysaccharide from A. pleuronectes	404:456	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	5	65	from	mice	1041:1044	arg1	groups					1061:1066	C, D, and E groups	1049:1066	C, D, and E groups	1049:1066	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	10	66	theme	E	1601:1601	arg1	groups					1603:1608	C, D, and E groups	1591:1608	C, D, and E groups	1591:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	2	67	theme	pre-column	625:634	arg1	method					651:656	PMP pre-column derivatization method	621:656	PMP pre-column derivatization method	621:656	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	9	68	theme	IFN-γ	1472:1476	arg1	levels					1462:1467	the serum levels	1452:1467	the serum levels of IFN-γ in C, D, and E groups	1452:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	2	69	theme	molecular	502:510	arg1	weight					512:517	the molecular weight	498:517	the molecular weight	498:517	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	8	70	from	pleuronectes	1395:1406	arg1	polysaccharide					1372:1385	purified polysaccharide	1363:1385	purified polysaccharide from A. pleuronectes	1363:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	8	70	from	pleuronectes	1395:1406	arg1	kDa					1417:1419	11.7 kDa	1412:1419	11.7 kDa	1412:1419	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	8	70	from	pleuronectes	1395:1406	arg1	weight					1353:1358	The molecular weight	1339:1358	The molecular weight of purified polysaccharide from A. pleuronectes	1339:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	9	71	theme	C	1481:1481	arg1	groups					1493:1498	C, D, and E groups	1481:1498	C, D, and E groups	1481:1498	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	10	72	theme	P	1652:1652	arg1	<					1654:1654	P < 0.01	1652:1659	P < 0.01	1652:1659	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	9	73	dep	F	1530:1530	arg1	<					1544:1544	P < 0.01	1542:1549	P < 0.01	1542:1549	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	1	74	theme	molecular	197:205	arg1	characterization					207:222	the molecular characterization	193:222	the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection	193:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	2	75	theme	pressure	578:585	arg1	chromatography					602:615	the high pressure size exclusion chromatography	569:615	the high pressure size exclusion chromatography	569:615	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	9	76	dep	increased	1519:1527	arg1	F					1530:1530	F	1530:1530	F = 63.525	1530:1539	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	0	77	from	pleuronectes	73:84	arg1	detection					32:40	molecular detection	22:40	molecular detection of polysaccharide from Amusium pleuronectes	22:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	2	78	theme	size	587:590	arg1	chromatography					602:615	the high pressure size exclusion chromatography	569:615	the high pressure size exclusion chromatography	569:615	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	0	79	from	fibrosis	118:125	arg1	rats					130:133	rats	130:133	rats infected with Schistosoma japonicum	130:169	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	13	80	theme	japonicum	2036:2044	arg1	infection					2046:2054	S. japonicum infection	2033:2054	S. japonicum infection	2033:2054	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	7	81	theme	HE	1229:1230	arg1	staining					1232:1239	HE staining	1229:1239	HE staining	1229:1239	HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits.					
30567018	2	82	theme	exclusion	592:600	arg1	chromatography					602:615	the high pressure size exclusion chromatography	569:615	the high pressure size exclusion chromatography	569:615	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	12	83	theme	P	1898:1898	arg1	<					1900:1900	P < 0.05	1898:1905	P < 0.05	1898:1905	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	5	84	theme	B	1142:1142	arg1	group					1144:1148	B group	1142:1148	B group	1142:1148	After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline.					
30567018	10	85	theme	B	1684:1684	arg1	group					1686:1690	B group	1684:1690	B group	1684:1690	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	11	86	theme	egg	1721:1723	arg1	nodules					1725:1731	the egg nodules	1717:1731	the egg nodules	1717:1731	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	4	87	theme	E	900:900	arg1	groups					902:907	B, C, D, or E groups	888:907	B, C, D, or E groups	888:907	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	1	88	theme	hepatic	344:350	arg1	fibrosis					352:359	hepatic fibrosis	344:359	hepatic fibrosis caused by Schistosoma japonicum infection	344:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	9	89	theme	=	1532:1532	arg1	F					1530:1530	F	1530:1530	F = 63.525	1530:1539	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	0	90	from	study	13:17	arg1	detection					32:40	molecular detection	22:40	molecular detection of polysaccharide from Amusium pleuronectes	22:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	3	91	theme	mice	701:704	arg1	total					675:679	A total	673:679	A total of 50 female BALB/c mice	673:704	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	4	92	dep	japonicum	974:982	arg1	±					988:988	30 ± 2	985:990	30 ± 2	985:990	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	13	93	theme	hepatic	1997:2003	arg1	fibrosis					2005:2012	hepatic fibrosis	1997:2012	hepatic fibrosis process induced by S. japonicum infection	1997:2054	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	1	94	theme	Schistosoma	371:381	arg1	infection					393:401	Schistosoma japonicum infection	371:401	Schistosoma japonicum infection	371:401	OBJECTIVE To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection.					
30567018	12	95	dep	χ2	1886:1887	arg1	<					1900:1900	P < 0.05	1898:1905	P < 0.05	1898:1905	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	13	96	theme	obvious	1968:1974	arg1	effect					1976:1981	an obvious effect	1965:1981	an obvious effect	1965:1981	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	2	97	theme	high	573:576	arg1	chromatography					602:615	the high pressure size exclusion chromatography	569:615	the high pressure size exclusion chromatography	569:615	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	8	98	theme	purified	1363:1370	arg1	polysaccharide					1372:1385	purified polysaccharide	1363:1385	purified polysaccharide from A. pleuronectes	1363:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	12	99	theme	nodules	1812:1818	arg1	number					1798:1803	The number	1794:1803	The number of egg nodules	1794:1818	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	12	99	theme	nodules	1812:1818	arg1	milder					1856:1861	milder	1856:1861	milder	1856:1861	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	12	99	theme	nodules	1812:1818	arg1	degree					1833:1838	fibrosis degree	1824:1838	fibrosis degree in E group	1824:1849	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	0	100	theme	molecular	22:30	arg1	detection					32:40	molecular detection	22:40	molecular detection of polysaccharide from Amusium pleuronectes	22:84	[Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum].					
30567018	11	101	theme	E	1784:1784	arg1	groups					1786:1791	B, C, D, and E groups	1771:1791	B, C, D, and E groups	1771:1791	HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups.					
30567018	10	102	dep	=	1642:1642	arg1	99.788					1644:1649	99.788	1644:1649	99.788	1644:1649	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	3	103	theme	female	687:692	arg1	mice					701:704	50 female BALB/c mice	684:704	50 female BALB/c mice	684:704	A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).					
30567018	12	104	theme	fibrosis	1824:1831	arg1	degree					1833:1838	fibrosis degree	1824:1838	fibrosis degree in E group	1824:1849	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	2	105	theme	A.	442:443	arg1	pleuronectes					445:456	A. pleuronectes	442:456	A. pleuronectes	442:456	METHODS The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively.					
30567018	8	106	from	weight	1353:1358	arg1	pleuronectes					1395:1406	A. pleuronectes	1392:1406	A. pleuronectes	1392:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	13	107	theme	A.	1945:1946	arg1	pleuronectes					1948:1959	A. pleuronectes	1945:1959	A. pleuronectes	1945:1959	CONCLUSIONS The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.					
30567018	10	108	theme	IL-13	1582:1586	arg1	levels					1572:1577	the serum levels	1562:1577	the serum levels of IL-13 in C, D, and E groups	1562:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	7	109	dep	serum	1274:1278	arg1	IFN-γ					1280:1284	IFN-γ	1280:1284	IFN-γ	1280:1284	HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits.					
30567018	7	109	dep	serum	1274:1278	arg1	IL-13					1290:1294	IL-13	1290:1294	IL-13	1290:1294	HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits.					
30567018	4	110	theme	abdominal	930:938	arg1	skin					940:943	the abdominal skin	926:943	the abdominal skin	926:943	The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively.					
30567018	9	111	theme	A	1436:1436	arg1	groups					1444:1449	A and B groups	1436:1449	A and B groups	1436:1449	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	10	112	theme	D	1594:1594	arg1	groups					1603:1608	C, D, and E groups	1591:1608	C, D, and E groups	1591:1608	However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group.					
30567018	12	113	from	number	1798:1803	arg1	group					1845:1849	E group	1843:1849	E group	1843:1849	The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05).					
30567018	7	114	theme	ELISA	1319:1323	arg1	kits					1325:1328	ELISA kits	1319:1328	ELISA kits	1319:1328	HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits.					
30567018	9	115	theme	B	1442:1442	arg1	groups					1444:1449	A and B groups	1436:1449	A and B groups	1436:1449	Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01).					
30567018	8	116	dep	RESULTS	1331:1337	arg1	kDa					1417:1419	11.7 kDa	1412:1419	11.7 kDa	1412:1419	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30567018	8	116	dep	RESULTS	1331:1337	arg1	weight					1353:1358	The molecular weight	1339:1358	The molecular weight of purified polysaccharide from A. pleuronectes	1339:1406	RESULTS The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa.					
30542567	0	0	theme	bisecting	90:98	arg1	GlcNAc					100:105	bisecting GlcNAc	90:105	bisecting GlcNAc	90:105	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	5	1	theme	glucosamine	766:776	arg1	unit					778:781	The bisecting glucosamine unit	752:781	The bisecting glucosamine unit	752:781	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	7	2	theme	interesting	1170:1180	arg1	preferences					1192:1202	interesting substrate preferences	1170:1202	interesting substrate preferences	1170:1202	Endo-A exhibited interesting substrate preferences depending on donor sizes, which was rationalized through molecular dynamics studies.					
30542567	6	3	gly	glycopeptide	1034:1045	arg2	glycopeptide					1034:1045	a glycopeptide acceptor	1032:1054	a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1032:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	3	4	theme	divergent	332:340	arg1	generation					342:351	divergent generation	332:351	divergent generation of N-glycan structures	332:374	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	7	5	theme	substrate	1182:1190	arg1	preferences					1192:1202	interesting substrate preferences	1170:1202	interesting substrate preferences	1170:1202	Endo-A exhibited interesting substrate preferences depending on donor sizes, which was rationalized through molecular dynamics studies.					
30542567	7	6	theme	molecular	1261:1269	arg1	studies					1280:1286	molecular dynamics studies	1261:1286	molecular dynamics studies	1261:1286	Endo-A exhibited interesting substrate preferences depending on donor sizes, which was rationalized through molecular dynamics studies.					
30542567	1	7	contain	have	131:134	arg2	structures					151:160	highly diverse structures	136:160	highly diverse structures	136:160	N-Linked glycopeptides have highly diverse structures in nature.					
30542567	1	7	contain	have	131:134	arg1	glycopeptides					117:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides have highly diverse structures in nature.					
30542567	2	8	theme	α1,3-linked	294:304	arg1	arms					314:317	both α1,6- and α1,3-linked mannose arms	279:317	both α1,6- and α1,3-linked mannose arms	279:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	8	9	theme	LewisX	1417:1422	arg1	antennae					1438:1445	two LewisX trisaccharide antennae	1413:1445	two LewisX trisaccharide antennae	1413:1445	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	4	10	theme	bisecting	603:611	arg1	moiety					625:630	a bisecting glucosamine moiety	601:630	a bisecting glucosamine moiety	601:630	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	7	11	theme	dynamics	1271:1278	arg1	studies					1280:1286	molecular dynamics studies	1261:1286	molecular dynamics studies	1261:1286	Endo-A exhibited interesting substrate preferences depending on donor sizes, which was rationalized through molecular dynamics studies.					
30542567	8	12	theme	trisaccharide	1424:1436	arg1	antennae					1438:1445	two LewisX trisaccharide antennae	1413:1445	two LewisX trisaccharide antennae	1413:1445	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	8	13	theme	rare	1387:1390	arg1	branch					1401:1406	the rare N-glycan branch	1383:1406	the rare N-glycan branch	1383:1406	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	1	14	gly	glycopeptides	117:129	arg2	glycopeptides					117:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides have highly diverse structures in nature.					
30542567	2	15	theme	α1,6-	284:288	arg1	arms					314:317	both α1,6- and α1,3-linked mannose arms	279:317	both α1,6- and α1,3-linked mannose arms	279:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	5	16	theme	modelling	939:947	arg1	analysis					949:956	molecular modelling analysis	929:956	molecular modelling analysis	929:956	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	8	17	theme	first	1301:1305	arg1	time					1307:1310	the first time	1297:1310	the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures	1297:1527	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	8	17	theme	first	1301:1305	arg1	This					1289:1292	This	1289:1292	This	1289:1292	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	3	18	theme	structures	365:374	arg1	generation					342:351	divergent generation	332:351	divergent generation of N-glycan structures	332:374	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	8	19	theme	structures	1518:1527	arg1	class					1488:1492	this class	1483:1492	this class of complex glycopeptide structures	1483:1527	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	3	20	theme	core	457:460	arg1	tetrasaccharide					462:476	the core tetrasaccharide	453:476	the core tetrasaccharide	453:476	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	4	21	from	flexible	582:589	arg1	addition					549:556	addition	549:556	addition	549:556	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	6	22	from	haptoglobin	1061:1071	arg1	acceptor					1047:1054	a glycopeptide acceptor	1032:1054	a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1032:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	6	23	theme	type	1094:1097	arg1	Endo-A					1144:1149	Endo-A	1144:1149	Endo-A	1144:1149	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	6	23	theme	type	1094:1097	arg1	endo-β-N-acetylglucosaminidase					1112:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase	1085:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1085:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	4	24	theme	synthesis	672:680	arg1	stage					659:663	a late stage	652:663	a late stage of the synthesis	652:680	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	0	25	theme	Chemoenzymatic	0:13	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of glycopeptides	0:40	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	6	26	theme	oxazoline	998:1006	arg1	donors					1008:1013	oxazoline donors	998:1013	oxazoline donors	998:1013	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	3	27	dep	one	533:535	arg1	another					537:543	another	537:543	another	537:543	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	6	28	theme	wild	1089:1092	arg1	Endo-A					1144:1149	Endo-A	1144:1149	Endo-A	1144:1149	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	6	28	theme	wild	1089:1092	arg1	endo-β-N-acetylglucosaminidase					1112:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase	1085:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1085:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	0	29	theme	glycopeptides	28:40	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of glycopeptides	0:40	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	4	30	theme	synthetic	563:571	arg1	route					573:577	the synthetic route	559:577	the synthetic route	559:577	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	4	30	theme	synthetic	563:571	arg1	flexible					582:589	flexible	582:589	flexible	582:589	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	0	31	gly	glycopeptides	28:40	arg2	glycopeptides					28:40	glycopeptides	28:40	glycopeptides	28:40	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	2	32	theme	arms	314:317	arg1	6-OH					271:274	6-OH	271:274	6-OH of both α1,6- and α1,3-linked mannose arms	271:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	6	33	theme	Arthrobacter	1099:1110	arg1	Endo-A					1144:1149	Endo-A	1144:1149	Endo-A	1144:1149	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	6	33	theme	Arthrobacter	1099:1110	arg1	endo-β-N-acetylglucosaminidase					1112:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase	1085:1141	the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1085:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	3	34	theme	protective	393:402	arg1	groups					404:409	four orthogonal protective groups	377:409	four orthogonal protective groups	377:409	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	2	35	link	α1,3-linked	294:304	arg1	arms					314:317	both α1,6- and α1,3-linked mannose arms	279:317	both α1,6- and α1,3-linked mannose arms	279:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	3	36	theme	N-glycan	356:363	arg1	structures					365:374	N-glycan structures	356:374	N-glycan structures	356:374	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	2	37	theme	mannose	306:312	arg1	arms					314:317	both α1,6- and α1,3-linked mannose arms	279:317	both α1,6- and α1,3-linked mannose arms	279:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	0	38	theme	rare	50:53	arg1	sequences					64:72	rare N-glycan sequences	50:72	rare N-glycan sequences	50:72	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	8	39	theme	bisecting	1342:1350	arg1	glucosamine					1361:1371	a bisecting N-acetyl glucosamine	1340:1371	a bisecting N-acetyl glucosamine (GlcNAc)	1340:1380	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	8	39	theme	bisecting	1342:1350	arg1	GlcNAc					1374:1379	GlcNAc	1374:1379	GlcNAc	1374:1379	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	2	40	theme	glycan	254:259	arg1	chains					261:266	glycan chains	254:266	glycan chains	254:266	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	8	41	gly	glycopeptide	1505:1516	arg2	glycopeptide					1505:1516	complex glycopeptide structures	1497:1527	complex glycopeptide structures	1497:1527	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	4	42	theme	late	654:657	arg1	stage					659:663	a late stage	652:663	a late stage of the synthesis	652:680	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	3	43	theme	branching	433:441	arg1	points					443:448	the branching points	429:448	the branching points on the core tetrasaccharide, which could be removed individually without affecting one another	429:543	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	7	44	theme	donor	1217:1221	arg1	sizes					1223:1227	donor sizes	1217:1227	donor sizes	1217:1227	Endo-A exhibited interesting substrate preferences depending on donor sizes, which was rationalized through molecular dynamics studies.					
30542567	8	45	theme	glycopeptide	1505:1516	arg1	structures					1518:1527	complex glycopeptide structures	1497:1527	complex glycopeptide structures	1497:1527	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	3	46	theme	orthogonal	382:391	arg1	groups					404:409	four orthogonal protective groups	377:409	four orthogonal protective groups	377:409	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	5	47	theme	adjacent	833:840	arg1	mannoses					842:849	adjacent mannoses	833:849	adjacent mannoses	833:849	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	4	48	from	addition	549:556	arg1	route					573:577	the synthetic route	559:577	the synthetic route	559:577	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	4	48	from	addition	549:556	arg1	flexible					582:589	flexible	582:589	flexible	582:589	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	5	49	theme	glycosylation	809:821	arg1	yields					823:828	the glycosylation yields	805:828	the glycosylation yields	805:828	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	8	50	theme	complex	1497:1503	arg1	structures					1518:1527	complex glycopeptide structures	1497:1527	complex glycopeptide structures	1497:1527	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	2	51	theme	N-glycan	237:244	arg1	synthesis					203:211	the first synthesis	193:211	the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms	193:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	8	52	theme	N-acetyl	1352:1359	arg1	glucosamine					1361:1371	a bisecting N-acetyl glucosamine	1340:1371	a bisecting N-acetyl glucosamine (GlcNAc)	1340:1380	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	8	52	theme	N-acetyl	1352:1359	arg1	GlcNAc					1374:1379	GlcNAc	1374:1379	GlcNAc	1374:1379	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	0	53	theme	N-glycan	55:62	arg1	sequences					64:72	rare N-glycan sequences	50:72	rare N-glycan sequences	50:72	Chemoenzymatic synthesis of glycopeptides bearing rare N-glycan sequences with or without bisecting GlcNAc.					
30542567	2	54	theme	rare	216:219	arg1	N-glycan					237:244	rare multi-antennary N-glycan	216:244	rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms	216:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	2	55	theme	multi-antennary	221:235	arg1	N-glycan					237:244	rare multi-antennary N-glycan	216:244	rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms	216:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	5	56	theme	molecular	929:937	arg1	analysis					949:956	molecular modelling analysis	929:956	molecular modelling analysis	929:956	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	8	57	dep	time	1307:1310	arg1	synthesized					1451:1461	synthesized	1451:1461	synthesized	1451:1461	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	8	58	gly	glycopeptide	1319:1330	arg2	glycopeptide					1319:1330	a glycopeptide	1317:1330	a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae	1317:1445	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	1	59	theme	diverse	143:149	arg1	structures					151:160	highly diverse structures	136:160	highly diverse structures	136:160	N-Linked glycopeptides have highly diverse structures in nature.					
30542567	1	60	theme	N-Linked	108:115	arg1	glycopeptides					117:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides	108:129	N-Linked glycopeptides have highly diverse structures in nature.					
30542567	8	61	theme	N-glycan	1392:1399	arg1	branch					1401:1406	the rare N-glycan branch	1383:1406	the rare N-glycan branch	1383:1406	This is the first time that a glycopeptide bearing a bisecting N-acetyl glucosamine (GlcNAc), the rare N-glycan branch, and two LewisX trisaccharide antennae was synthesized, enabling access to this class of complex glycopeptide structures.					
30542567	3	62	from	points	443:448	arg1	tetrasaccharide					462:476	the core tetrasaccharide	453:476	the core tetrasaccharide	453:476	To expedite divergent generation of N-glycan structures, four orthogonal protective groups were installed at the branching points on the core tetrasaccharide, which could be removed individually without affecting one another.					
30542567	4	63	theme	sequences	718:726	arg1	diversity					705:713	the diversity	701:713	the diversity of sequences that could be achieved	701:749	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	4	64	theme	glucosamine	613:623	arg1	moiety					625:630	a bisecting glucosamine moiety	601:630	a bisecting glucosamine moiety	601:630	In addition, the synthetic route is flexible, allowing a bisecting glucosamine moiety to be introduced at a late stage of the synthesis, further expanding the diversity of sequences that could be achieved.					
30542567	2	65	theme	first	197:201	arg1	synthesis					203:211	the first synthesis	193:211	the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms	193:317	Herein, we describe the first synthesis of rare multi-antennary N-glycan bearing glycan chains on 6-OH of both α1,6- and α1,3-linked mannose arms.					
30542567	5	66	theme	steric	876:881	arg1	hindrance					883:891	steric hindrance	876:891	steric hindrance imposed by the glucosamine based on molecular modelling analysis	876:956	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	6	67	theme	glycopeptide	1034:1045	arg1	acceptor					1047:1054	a glycopeptide acceptor	1032:1054	a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A)	1032:1150	The N-glycans were then transformed to oxazoline donors and ligated with a glycopeptide acceptor from haptoglobin promoted by the wild type Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A).					
30542567	5	68	theme	bisecting	756:764	arg1	unit					778:781	The bisecting glucosamine unit	752:781	The bisecting glucosamine unit	752:781	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30542567	5	69	theme	mannoses	842:849	arg1	yields					823:828	the glycosylation yields	805:828	the glycosylation yields	805:828	The bisecting glucosamine unit significantly reduced the glycosylation yields of adjacent mannoses, which was attributed to steric hindrance imposed by the glucosamine based on molecular modelling analysis.					
30987249	2	0	theme	elements	519:526	arg1	increment					494:502	an increment	491:502	an increment of macro/micro elements	491:526	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	3	1	theme	present	750:756	arg1	xylose					649:654	xylose	649:654	xylose	649:654	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	extract					741:747	3.2-27.3 mg/glyophilized extract	716:747	3.2-27.3 mg/glyophilized extract	716:747	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	fucose					657:662	fucose	657:662	fucose	657:662	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	Galactose					629:637	Galactose	629:637	Galactose	629:637	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	mannose					640:646	mannose	640:646	mannose	640:646	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	1	theme	present	750:756	arg1	acid					680:683	glucuronic acid	669:683	glucuronic acid	669:683	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	2	from	ratios	776:781	arg1	present					750:756	present	750:756	present	750:756	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	3	dep	monosaccharides	699:713	arg1	whereas					784:790	whereas	784:790	whereas	784:790	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	2	4	theme	muticum	372:378	arg1	extracts					405:412	Sargassum muticum and Osmundea pinnatifida extracts	362:412	Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively	362:481	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	6	5	theme	nutritional	1358:1368	arg1	purposes					1391:1398	nutritional and/or nutraceutical purposes	1358:1398	nutritional and/or nutraceutical purposes	1358:1398	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	2	6	theme	macro/micro	507:517	arg1	elements					519:526	macro/micro elements	507:526	macro/micro elements	507:526	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	4	7	from	presence	930:937	arg1	muticum					1020:1026	S. muticum	1017:1026	S. muticum	1017:1026	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	7	from	presence	930:937	arg1	extracts					985:992	the extracts	981:992	the extracts	981:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	6	8	dep	composition	1246:1256	arg1	the					1242:1244	the	1242:1244	the	1242:1244	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	4	9	from	O.	1072:1073	arg1	polysaccharides					1051:1065	sulfated polysaccharides	1042:1065	sulfated polysaccharides from O. pinnatifida	1042:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	5	10	theme	normal	1112:1117	arg1	cells					1129:1133	normal mammalian cells	1112:1133	normal mammalian cells	1112:1133	No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts.					
30987249	1	11	theme	enzyme-assisted	243:257	arg1	method					290:295	an efficient method	277:295	an efficient method to obtain multifunctional extracts	277:330	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	1	11	theme	enzyme-assisted	243:257	arg1	extraction					259:268	enzyme-assisted extraction	243:268	enzyme-assisted extraction	243:268	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	0	12	theme	Cytotoxic	89:97	arg1	Characterization					99:114	Cytotoxic Characterization	89:114	Cytotoxic Characterization	89:114	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	3	13	theme	main	694:697	arg1	xylose					649:654	xylose	649:654	xylose	649:654	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	extract					741:747	3.2-27.3 mg/glyophilized extract	716:747	3.2-27.3 mg/glyophilized extract	716:747	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	fucose					657:662	fucose	657:662	fucose	657:662	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	Galactose					629:637	Galactose	629:637	Galactose	629:637	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	mannose					640:646	mannose	640:646	mannose	640:646	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	13	theme	main	694:697	arg1	acid					680:683	glucuronic acid	669:683	glucuronic acid	669:683	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	6	14	theme	comprehensive	1208:1220	arg1	characterization					1222:1237	The comprehensive characterization	1204:1237	The comprehensive characterization of the composition and safety of these two extracts	1204:1289	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	4	15	from	structures	967:976	arg1	extracts					985:992	the extracts	981:992	the extracts	981:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	2	16	theme	corresponding	549:561	arg1	seaweeds					567:574	the corresponding dry seaweeds	545:574	the corresponding dry seaweeds	545:574	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	5	17	theme	mammalian	1119:1127	arg1	cells					1129:1133	normal mammalian cells	1112:1133	normal mammalian cells	1112:1133	No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts.					
30987249	4	18	from	extracts	985:992	arg1	presence					930:937	the presence	926:937	the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida	926:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	6	19	theme	important	1302:1310	arg1	step					1312:1315	an important step	1299:1315	an important step towards their authorized application for nutritional and/or nutraceutical purposes	1299:1398	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	1	20	used	used	150:153	arg2	Seaweeds					117:124	Seaweeds	117:124	Seaweeds	117:124	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	6	21	theme	nutraceutical	1377:1389	arg1	purposes					1391:1398	nutritional and/or nutraceutical purposes	1358:1398	nutritional and/or nutraceutical purposes	1358:1398	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	3	22	from	present	750:756	arg1	ratios					776:781	variable molar ratios	761:781	variable molar ratios	761:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	1	23	theme	human	159:163	arg1	consumption					165:175	human consumption	159:175	human consumption	159:175	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	3	24	theme	3.2-27.3	716:723	arg1	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	24	theme	3.2-27.3	716:723	arg1	extract					741:747	3.2-27.3 mg/glyophilized extract	716:747	3.2-27.3 mg/glyophilized extract	716:747	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	4	25	theme	agarans	1031:1037	arg1	presence					930:937	the presence	926:937	the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida	926:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	5	26	dep	4	1158:1158	arg1	to					1155:1156	to	1155:1156	to	1155:1156	No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts.					
30987249	0	27	theme	muticum	10:16	arg1	Extracts					53:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts	0:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.	0:115	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	3	28	theme	mg/glyophilized	725:739	arg1	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	28	theme	mg/glyophilized	725:739	arg1	extract					741:747	3.2-27.3 mg/glyophilized extract	716:747	3.2-27.3 mg/glyophilized extract	716:747	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	6	29	theme	extracts	1282:1289	arg1	safety					1262:1267	safety	1262:1267	safety	1262:1267	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	6	29	theme	extracts	1282:1289	arg1	composition					1246:1256	composition	1246:1256	composition	1246:1256	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	4	30	theme	structures	967:976	arg1	presence					930:937	the presence	926:937	the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida	926:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	31	theme	1H	901:902	arg1	spectra					908:914	1H NMR spectra	901:914	1H NMR spectra	901:914	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	2	32	theme	extracts	405:412	arg1	characterization					342:357	Chemical characterization	333:357	Chemical characterization	333:357	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	0	33	theme	Sargassum	0:8	arg1	muticum					10:16	Sargassum muticum	0:16	Sargassum muticum	0:16	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	3	34	theme	variable	761:768	arg1	ratios					776:781	variable molar ratios	761:781	variable molar ratios	761:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	35	theme	low	792:794	arg1	acids					807:811	low free amino acids content and diversity	792:833	low free amino acids content and diversity (1.4-2.7 g/100gprotein)	792:857	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	35	theme	low	792:794	arg1	diversity					825:833	diversity	825:833	diversity	825:833	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	35	theme	low	792:794	arg1	g/100gprotein					844:856	1.4-2.7 g/100gprotein	836:856	1.4-2.7 g/100gprotein	836:856	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	35	theme	low	792:794	arg1	content					813:819	content	813:819	content	813:819	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	2	36	theme	pinnatifida	393:403	arg1	extracts					405:412	Sargassum muticum and Osmundea pinnatifida extracts	362:412	Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively	362:481	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	0	37	theme	Osmundea	22:29	arg1	pinnatifida					31:41	Osmundea pinnatifida	22:41	Osmundea pinnatifida	22:41	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	3	38	theme	free	796:799	arg1	acids					807:811	low free amino acids content and diversity	792:833	low free amino acids content and diversity (1.4-2.7 g/100gprotein)	792:857	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	38	theme	free	796:799	arg1	diversity					825:833	diversity	825:833	diversity	825:833	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	38	theme	free	796:799	arg1	g/100gprotein					844:856	1.4-2.7 g/100gprotein	836:856	1.4-2.7 g/100gprotein	836:856	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	38	theme	free	796:799	arg1	content					813:819	content	813:819	content	813:819	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	2	39	theme	assisted	449:456	arg1	extraction					458:467	Viscozyme assisted extraction	439:467	Viscozyme assisted extraction	439:467	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	4	40	theme	sulfated	1042:1049	arg1	polysaccharides					1051:1065	sulfated polysaccharides	1042:1065	sulfated polysaccharides from O. pinnatifida	1042:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	1	41	theme	efficient	280:288	arg1	method					290:295	an efficient method	277:295	an efficient method to obtain multifunctional extracts	277:330	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	1	41	theme	efficient	280:288	arg1	extraction					259:268	enzyme-assisted extraction	243:268	enzyme-assisted extraction	243:268	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	2	42	theme	Viscozyme	439:447	arg1	extraction					458:467	Viscozyme assisted extraction	439:467	Viscozyme assisted extraction	439:467	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	0	43	theme	Enzymatic	43:51	arg1	Extracts					53:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts	0:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.	0:115	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	4	44	from	agarans	1031:1037	arg1	extracts					985:992	the extracts	981:992	the extracts	981:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	45	theme	important	942:950	arg1	structures					967:976	important polysaccharide structures	942:976	important polysaccharide structures in the extracts	942:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	1	46	theme	potential	195:203	arg1	source					205:210	a potential source	193:210	a potential source	193:210	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	0	47	theme	pinnatifida	31:41	arg1	Extracts					53:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts	0:60	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.	0:115	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	5	48	theme	mglyophilized	1160:1172	arg1	extract/mL					1174:1183	0 to 4 mglyophilized extract/mL	1153:1183	0 to 4 mglyophilized extract/mL	1153:1183	No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts.					
30987249	3	49	dep	acids	807:811	arg1	acids					807:811	low free amino acids content and diversity	792:833	low free amino acids content and diversity (1.4-2.7 g/100gprotein)	792:857	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	49	dep	acids	807:811	arg1	diversity					825:833	diversity	825:833	diversity	825:833	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	49	dep	acids	807:811	arg1	content					813:819	content	813:819	content	813:819	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	0	50	dep	Extracts	53:60	arg1	Structural					73:82	Structural	73:82	Structural	73:82	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	0	50	dep	Extracts	53:60	arg1	Chemical					63:70	Chemical	63:70	Chemical	63:70	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	0	50	dep	Extracts	53:60	arg1	Characterization					99:114	Cytotoxic Characterization	89:114	Cytotoxic Characterization	89:114	Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization.					
30987249	6	51	theme	authorized	1331:1340	arg1	application					1342:1352	their authorized application	1325:1352	their authorized application for nutritional and/or nutraceutical purposes	1325:1398	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	2	52	theme	dry	563:565	arg1	seaweeds					567:574	the corresponding dry seaweeds	545:574	the corresponding dry seaweeds	545:574	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	4	53	theme	NMR	904:906	arg1	spectra					908:914	1H NMR spectra	901:914	1H NMR spectra	901:914	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	3	54	theme	molar	770:774	arg1	ratios					776:781	variable molar ratios	761:781	variable molar ratios	761:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	6	55	theme	safety	1262:1267	arg1	characterization					1222:1237	The comprehensive characterization	1204:1237	The comprehensive characterization of the composition and safety of these two extracts	1204:1289	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	1	56	theme	multifunctional	307:321	arg1	extracts					323:330	multifunctional extracts	307:330	multifunctional extracts	307:330	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	2	57	theme	Na/K	596:599	arg1	ratio					587:591	the ratio	583:591	the ratio of Na/K	583:599	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	4	58	from	muticum	1020:1026	arg1	presence					930:937	the presence	926:937	the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida	926:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	58	from	muticum	1020:1026	arg1	structures					967:976	important polysaccharide structures	942:976	important polysaccharide structures in the extracts	942:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	58	from	muticum	1020:1026	arg1	agarans					1031:1037	agarans	1031:1037	agarans as sulfated polysaccharides from O. pinnatifida	1031:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	4	58	from	muticum	1020:1026	arg1	fucoidans					1002:1010	fucoidans	1002:1010	fucoidans from S. muticum	1002:1026	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	6	59	theme	composition	1246:1256	arg1	characterization					1222:1237	The comprehensive characterization	1204:1237	The comprehensive characterization of the composition and safety of these two extracts	1204:1289	The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.					
30987249	2	60	theme	Chemical	333:340	arg1	characterization					342:357	Chemical characterization	333:357	Chemical characterization	333:357	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
30987249	4	61	from	fucoidans	1002:1010	arg1	extracts					985:992	the extracts	981:992	the extracts	981:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	3	62	attach	present	750:756	arg2	mannose					640:646	mannose	640:646	mannose	640:646	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg1	ratios					776:781	variable molar ratios	761:781	variable molar ratios	761:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	acid					680:683	glucuronic acid	669:683	glucuronic acid	669:683	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	extract					741:747	3.2-27.3 mg/glyophilized extract	716:747	3.2-27.3 mg/glyophilized extract	716:747	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	xylose					649:654	xylose	649:654	xylose	649:654	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	Galactose					629:637	Galactose	629:637	Galactose	629:637	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	62	attach	present	750:756	arg2	fucose					657:662	fucose	657:662	fucose	657:662	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	4	63	theme	polysaccharide	952:965	arg1	structures					967:976	important polysaccharide structures	942:976	important polysaccharide structures in the extracts	942:992	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	1	64	theme	biological	215:224	arg1	compounds					226:234	biological compounds	215:234	biological compounds	215:234	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	xylose					649:654	xylose	649:654	xylose	649:654	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	fucose					657:662	fucose	657:662	fucose	657:662	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	Galactose					629:637	Galactose	629:637	Galactose	629:637	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	monosaccharides					699:713	the main monosaccharides	690:713	the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios	690:781	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	mannose					640:646	mannose	640:646	mannose	640:646	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	65	theme	glucuronic	669:678	arg1	acid					680:683	glucuronic acid	669:683	glucuronic acid	669:683	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	1	66	theme	compounds	226:234	arg1	source					205:210	a potential source	193:210	a potential source	193:210	Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts.					
30987249	4	67	theme	fucoidans	1002:1010	arg1	presence					930:937	the presence	926:937	the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida	926:1085	FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida.					
30987249	3	68	theme	amino	801:805	arg1	acids					807:811	low free amino acids content and diversity	792:833	low free amino acids content and diversity (1.4-2.7 g/100gprotein)	792:857	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	68	theme	amino	801:805	arg1	diversity					825:833	diversity	825:833	diversity	825:833	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	68	theme	amino	801:805	arg1	g/100gprotein					844:856	1.4-2.7 g/100gprotein	836:856	1.4-2.7 g/100gprotein	836:856	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	3	68	theme	amino	801:805	arg1	content					813:819	content	813:819	content	813:819	Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts.					
30987249	2	69	dep	seaweeds	567:574	arg1	comparison					531:540	comparison	531:540	comparison	531:540	Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts.					
31858418	7	0	dep	pesticides	1035:1044	arg1	pesticides					1035:1044	the pesticides solubility and improvement	1031:1071	the pesticides solubility and improvement	1031:1071	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	7	0	dep	pesticides	1035:1044	arg1	improvement					1061:1071	improvement	1061:1071	improvement	1061:1071	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	7	0	dep	pesticides	1035:1044	arg1	solubility					1046:1055	solubility	1046:1055	solubility	1046:1055	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	1	1	theme	systems	257:263	arg1	development					213:223	the development	209:223	the development of nanosized controlled release systems, such as polymer nanoparticles, micelles	209:304	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	0	2	theme	plant	84:88	arg1	protection					90:99	plant protection	84:99	plant protection	84:99	Arabinogalactan and glycyrrhizin based nanopesticides as novel delivery systems for plant protection.					
31858418	1	3	theme	wide	325:328	arg1	materials					341:349	materials	341:349	materials	341:349	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	3	theme	wide	325:328	arg1	variety					330:336	a wide variety	323:336	a wide variety of materials	323:349	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	7	4	theme	penetration	1080:1090	arg1	Enhancement					1016:1026	Enhancement	1016:1026	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds	1016:1127	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	9	5	theme	grains	1531:1536	arg1	surface					1520:1526	the surface	1516:1526	the surface of grains	1516:1536	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	4	6	theme	barley	725:730	arg1	seeds					742:746	the barley and wheat seeds	721:746	seeds	742:746	Enhancement of the NA solubility and penetration into the barley and wheat seeds had been demonstrated.					
31858418	2	7	theme	natural	506:512	arg1	polysaccharides					514:528	natural polysaccharides	506:528	natural polysaccharides	506:528	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	9	8	theme	membranes	1568:1576	arg1	affinity					1497:1504	affinity	1497:1504	affinity of DSs to the surface of grains	1497:1536	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	9	8	theme	membranes	1568:1576	arg1	modification					1547:1558	the modification	1543:1558	the modification of cell membranes by poly- and oligosaccharides	1543:1606	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	9	8	theme	membranes	1568:1576	arg1	enhancement					1484:1494	the solubility enhancement	1469:1494	the solubility enhancement	1469:1494	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	5	9	used	used	808:811	arg2	DSs					799:801	these DSs	793:801	these DSs	793:801	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	4	10	theme	wheat	736:740	arg1	seeds					742:746	the barley and wheat seeds	721:746	seeds	742:746	Enhancement of the NA solubility and penetration into the barley and wheat seeds had been demonstrated.					
31858418	8	11	theme	strongest	1152:1160	arg1	enhancement					1162:1172	The strongest enhancement	1148:1172	The strongest enhancement of penetration ability	1148:1195	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	1	12	theme	materials	341:349	arg1	materials					341:349	materials	341:349	materials	341:349	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	12	theme	materials	341:349	arg1	variety					330:336	a wide variety	323:336	a wide variety of materials	323:349	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	9	13	theme	nanopesticide	1418:1430	arg1	penetration					1432:1442	the nanopesticide penetration	1414:1442	the nanopesticide penetration	1414:1442	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	8	14	theme	arabinogalactan	1214:1228	arg1	nanocomposites					1230:1243	arabinogalactan nanocomposites	1214:1243	arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz	1214:1338	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	8	14	theme	arabinogalactan	1214:1228	arg1	10-folds					1303:1310	10-folds	1303:1310	10-folds	1303:1310	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	8	14	theme	arabinogalactan	1214:1228	arg1	5-folds					1246:1252	5-folds	1246:1252	5-folds	1246:1252	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	7	15	theme	corn	1110:1113	arg1	seeds					1101:1105	the seeds	1097:1105	the seeds of corn and rapeseeds	1097:1127	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	9	16	theme	oligosaccharides	1394:1409	arg1	effect					1364:1369	the effect	1360:1369	the effect of polysaccharides and oligosaccharides on the nanopesticide penetration	1360:1442	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	2	17	theme	pesticides	368:377	arg1	penetration					379:389	the pesticides penetration	364:389	the pesticides penetration into the grain	364:404	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	5	18	theme	present	778:784	arg1	study					786:790	the present study	774:790	the present study	774:790	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	2	19	with	preparation	489:499	arg1	oligosaccharides					534:549	oligosaccharides	534:549	oligosaccharides as delivery systems (DSs)	534:575	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	2	19	with	preparation	489:499	arg1	polysaccharides					514:528	natural polysaccharides	506:528	natural polysaccharides	506:528	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	1	20	theme	plant	175:179	arg1	products					192:199	plant protection products	175:199	plant protection products	175:199	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	2	21	theme	seed	426:429	arg1	treatment					431:439	the presowing seed treatment	412:439	the presowing seed treatment	412:439	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	1	22	theme	polymer	274:280	arg1	nanoparticles					282:294	polymer nanoparticles	274:294	polymer nanoparticles	274:294	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	8	23	dep	nanocomposites	1230:1243	arg1	nanocomposites					1230:1243	arabinogalactan nanocomposites	1214:1243	arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz	1214:1338	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	8	23	dep	nanocomposites	1230:1243	arg1	10-folds					1303:1310	10-folds	1303:1310	10-folds	1303:1310	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	8	23	dep	nanocomposites	1230:1243	arg1	5-folds					1246:1252	5-folds	1246:1252	5-folds	1246:1252	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	1	24	theme	protection	181:190	arg1	products					192:199	plant protection products	175:199	plant protection products	175:199	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	2	25	theme	presowing	416:424	arg1	treatment					431:439	the presowing seed treatment	412:439	the presowing seed treatment	412:439	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	2	26	theme	delivery	554:561	arg1	DSs					572:574	DSs	572:574	DSs	572:574	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	2	26	theme	delivery	554:561	arg1	systems					563:569	delivery systems	554:569	delivery systems (DSs)	554:575	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	6	27	with	formation	919:927	arg1	oligosaccharides					962:977	oligosaccharides	962:977	oligosaccharides	962:977	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	6	27	with	formation	919:927	arg1	poly-					952:956	poly-	952:956	poly-	952:956	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	1	28	theme	products	192:199	arg1	delivery					163:170	delivery	163:170	delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles	163:304	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	2	29	dep	preparation	489:499	arg1	associate					479:487	associate	479:487	associate	479:487	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	3	30	theme	antidote	627:634	arg1	NA					662:663	NA	662:663	NA	662:663	Earlier, this approach was tested on antidote 1,8-naphthalic anhydride (NA).					
31858418	3	30	theme	antidote	627:634	arg1	anhydride					651:659	antidote 1,8-naphthalic anhydride	627:659	antidote 1,8-naphthalic anhydride (NA)	627:664	Earlier, this approach was tested on antidote 1,8-naphthalic anhydride (NA).					
31858418	1	31	theme	past	113:116	arg1	decade					118:123	the past decade	109:123	the past decade	109:123	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	8	32	theme	ability	1189:1195	arg1	enhancement					1162:1172	The strongest enhancement	1148:1172	The strongest enhancement of penetration ability	1148:1195	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	3	33	theme	1,8-naphthalic	636:649	arg1	NA					662:663	NA	662:663	NA	662:663	Earlier, this approach was tested on antidote 1,8-naphthalic anhydride (NA).					
31858418	3	33	theme	1,8-naphthalic	636:649	arg1	anhydride					651:659	antidote 1,8-naphthalic anhydride	627:659	antidote 1,8-naphthalic anhydride (NA)	627:664	Earlier, this approach was tested on antidote 1,8-naphthalic anhydride (NA).					
31858418	5	34	theme	pesticides	842:851	arg1	nanocomposites					824:837	nanocomposites	824:837	nanocomposites of pesticides	824:851	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	5	34	theme	pesticides	842:851	arg1	pesticides					842:851	pesticides	842:851	pesticides	842:851	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	9	35	theme	solubility	1473:1482	arg1	enhancement					1484:1494	the solubility enhancement	1469:1494	the solubility enhancement	1469:1494	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	2	36	theme	new	444:446	arg1	approach					448:455	a new approach	442:455	a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs)	442:575	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	6	37	theme	composite	909:917	arg1	formation					919:927	The composite formation	905:927	The composite formation of the pesticides with poly- and oligosaccharides	905:977	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	7	38	theme	rapeseeds	1119:1127	arg1	seeds					1101:1105	the seeds	1097:1105	the seeds of corn and rapeseeds	1097:1127	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	9	39	theme	polysaccharides	1374:1388	arg1	effect					1364:1369	the effect	1360:1369	the effect of polysaccharides and oligosaccharides on the nanopesticide penetration	1360:1442	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	6	40	theme	relaxation	997:1006	arg1	method					1008:1013	NMR relaxation method	993:1013	NMR relaxation method	993:1013	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	0	41	theme	delivery	63:70	arg1	systems					72:78	novel delivery systems	57:78	novel delivery systems for plant protection	57:99	Arabinogalactan and glycyrrhizin based nanopesticides as novel delivery systems for plant protection.					
31858418	8	42	theme	penetration	1177:1187	arg1	ability					1189:1195	penetration ability	1177:1195	penetration ability	1177:1195	The strongest enhancement of penetration ability was observed for arabinogalactan nanocomposites: 5-folds for tebuconazole and imidacloprid, and more than 10-folds for imazalil and prochloraz.					
31858418	5	43	dep	prepare	816:822	arg1	imazalil					882:889	imazalil	882:889	imazalil	882:889	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	5	43	dep	prepare	816:822	arg1	imidacloprid					868:879	imidacloprid	868:879	imidacloprid	868:879	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	5	43	dep	prepare	816:822	arg1	tebuconazole					854:865	tebuconazole	854:865	tebuconazole	854:865	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	5	43	dep	prepare	816:822	arg1	prochloraz					892:901	prochloraz	892:901	prochloraz	892:901	In the present study, these DSs were used to prepare nanocomposites of pesticides (tebuconazole, imidacloprid, imazalil, prochloraz).					
31858418	4	44	theme	solubility	689:698	arg1	Enhancement					667:677	Enhancement	667:677	Enhancement of the NA solubility	667:698	Enhancement of the NA solubility and penetration into the barley and wheat seeds had been demonstrated.					
31858418	4	44	theme	solubility	689:698	arg1	penetration					704:714	penetration	704:714	penetration into the barley and wheat seeds	704:746	Enhancement of the NA solubility and penetration into the barley and wheat seeds had been demonstrated.					
31858418	0	45	theme	novel	57:61	arg1	systems					72:78	novel delivery systems	57:78	novel delivery systems for plant protection	57:99	Arabinogalactan and glycyrrhizin based nanopesticides as novel delivery systems for plant protection.					
31858418	7	46	theme	pesticides	1035:1044	arg1	Enhancement					1016:1026	Enhancement	1016:1026	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds	1016:1127	Enhancement of the pesticides solubility and improvement of its penetration into the seeds of corn and rapeseeds has been detected.					
31858418	4	47	theme	NA	686:687	arg1	solubility					689:698	the NA solubility	682:698	the NA solubility	682:698	Enhancement of the NA solubility and penetration into the barley and wheat seeds had been demonstrated.					
31858418	9	48	from	effect	1364:1369	arg1	penetration					1432:1442	the nanopesticide penetration	1414:1442	the nanopesticide penetration	1414:1442	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	9	49	theme	cell	1563:1566	arg1	membranes					1568:1576	cell membranes	1563:1576	cell membranes	1563:1576	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	6	50	theme	NMR	993:995	arg1	method					1008:1013	NMR relaxation method	993:1013	NMR relaxation method	993:1013	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	9	51	theme	DSs	1509:1511	arg1	affinity					1497:1504	affinity	1497:1504	affinity of DSs to the surface of grains	1497:1536	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	9	51	theme	DSs	1509:1511	arg1	modification					1547:1558	the modification	1543:1558	the modification of cell membranes by poly- and oligosaccharides	1543:1606	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	9	51	theme	DSs	1509:1511	arg1	enhancement					1484:1494	the solubility enhancement	1469:1494	the solubility enhancement	1469:1494	Our data show that the effect of polysaccharides and oligosaccharides on the nanopesticide penetration might be associated with the solubility enhancement, affinity of DSs to the surface of grains, and the modification of cell membranes by poly- and oligosaccharides.					
31858418	1	52	theme	nanosized	228:236	arg1	micelles					297:304	micelles	297:304	micelles	297:304	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	52	theme	nanosized	228:236	arg1	nanoparticles					282:294	polymer nanoparticles	274:294	polymer nanoparticles	274:294	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	52	theme	nanosized	228:236	arg1	systems					257:263	nanosized controlled release systems	228:263	nanosized controlled release systems	228:263	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	53	theme	controlled	238:247	arg1	micelles					297:304	micelles	297:304	micelles	297:304	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	53	theme	controlled	238:247	arg1	nanoparticles					282:294	polymer nanoparticles	274:294	polymer nanoparticles	274:294	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	53	theme	controlled	238:247	arg1	systems					257:263	nanosized controlled release systems	228:263	nanosized controlled release systems	228:263	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	2	54	theme	non-covalent	466:477	arg1	preparation					489:499	non-covalent associate preparation	466:499	non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs)	466:575	To increase the pesticides penetration into the grain under the presowing seed treatment, a new approach based on non-covalent associate preparation with natural polysaccharides and oligosaccharides as delivery systems (DSs) was applied.					
31858418	6	55	theme	pesticides	936:945	arg1	formation					919:927	The composite formation	905:927	The composite formation of the pesticides with poly- and oligosaccharides	905:977	The composite formation of the pesticides with poly- and oligosaccharides was proved by NMR relaxation method.					
31858418	1	56	theme	new	152:154	arg1	era					156:158	a new era	150:158	a new era	150:158	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	57	theme	release	249:255	arg1	micelles					297:304	micelles	297:304	micelles	297:304	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	57	theme	release	249:255	arg1	nanoparticles					282:294	polymer nanoparticles	274:294	polymer nanoparticles	274:294	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
31858418	1	57	theme	release	249:255	arg1	systems					257:263	nanosized controlled release systems	228:263	nanosized controlled release systems	228:263	During the past decade, nanotechnologies opened a new era in delivery of plant protection products through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials.					
30102859	4	0	theme	alkaline	872:879	arg1	solution					881:888	alkaline solution	872:888	alkaline solution	872:888	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	1	1	theme	chemical	149:156	arg1	analysis					170:177	chemical composition analysis	149:177	chemical composition analysis	149:177	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	0	2	theme	Bamboo	81:86	arg1	Culms					114:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	3	theme	composition	158:168	arg1	analysis					170:177	chemical composition analysis	149:177	chemical composition analysis	149:177	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	6	4	theme	NMR	993:995	arg1	analyses					997:1004	FT-IR and NMR analyses	983:1004	analyses	997:1004	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	0	5	theme	affinis	105:111	arg1	Culms					114:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	0	6	from	Changes	0:6	arg1	Culms					114:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	3	7	theme	secondary	574:582	arg1	wall					589:592	the secondary cell wall	570:592	the secondary cell wall	570:592	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	0	8	theme	Neosinocalamus	90:103	arg1	Culms					114:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	9	from	differences	134:144	arg1	analysis					170:177	chemical composition analysis	149:177	chemical composition analysis	149:177	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	1	9	from	differences	134:144	arg1	distribution					191:202	spatial distribution	183:202	spatial distribution	183:202	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	6	10	theme	substituted	1100:1110	arg1	xylans					1112:1117	xylans	1112:1117	xylans	1112:1117	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	1	11	theme	standard	267:274	arg1	methods					256:262	the methods	252:262	the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy	252:344	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	4	12	theme	culms	852:856	arg1	holocellulose					772:784	holocellulose	772:784	holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms	772:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	3	13	from	intensity	542:550	arg1	regions					626:632	the compound middle lamella regions	598:632	the compound middle lamella regions	598:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	3	13	from	intensity	542:550	arg1	wall					589:592	the secondary cell wall	570:592	the secondary cell wall	570:592	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	5	14	theme	bamboo	975:980	arg1	growth					965:970	the growth	961:970	the growth of bamboo	961:980	The ratio of arabinose to xylose of hemicelluloses was increased with the growth of bamboo.					
30102859	2	15	theme	acid-soluble	383:394	arg1	lignin					396:401	the acid-soluble lignin	379:401	the acid-soluble lignin	379:401	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	6	16	theme	bamboo	1057:1062	arg1	culms					1064:1068	young bamboo culms	1051:1068	young bamboo culms	1051:1068	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	6	17	theme	FT-IR	983:987	arg1	analyses					997:1004	FT-IR and NMR analyses	983:1004	analyses	997:1004	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	5	18	theme	arabinose	904:912	arg1	ratio					895:899	The ratio	891:899	The ratio of arabinose to xylose of hemicelluloses	891:940	The ratio of arabinose to xylose of hemicelluloses was increased with the growth of bamboo.					
30102859	4	19	theme	bamboo	845:850	arg1	culms					852:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	3	20	theme	lignin	555:560	arg1	intensity					542:550	Raman signal intensity	529:550	Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions	529:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	3	21	theme	cell	584:587	arg1	wall					589:592	the secondary cell wall	570:592	the secondary cell wall	570:592	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	1	22	theme	spatial	183:189	arg1	distribution					191:202	spatial distribution	183:202	spatial distribution	183:202	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	3	23	theme	bamboo	681:686	arg1	growth					671:676	growth	671:676	growth of bamboo	671:686	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	0	24	theme	Composition	20:30	arg1	Changes					0:6	Changes	0:6	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.	0:119	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	25	theme	National	279:286	arg1	Laboratory					305:314	National Renewable Energy Laboratory	279:314	National Renewable Energy Laboratory	279:314	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	5	26	theme	hemicelluloses	927:940	arg1	xylose					917:922	xylose	917:922	xylose of hemicelluloses	917:940	The ratio of arabinose to xylose of hemicelluloses was increased with the growth of bamboo.					
30102859	6	27	theme	young	1051:1055	arg1	culms					1064:1068	young bamboo culms	1051:1068	young bamboo culms	1051:1068	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	4	28	theme	12-month-old	809:820	arg1	culms					852:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	6	29	theme	culms	1064:1068	arg1	hemicelluloses					1033:1046	DMSO-soluble hemicelluloses	1020:1046	DMSO-soluble hemicelluloses of young bamboo culms	1020:1068	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	0	30	theme	Chemical	11:18	arg1	Composition					20:30	Chemical Composition	11:30	Chemical Composition	11:30	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	31	theme	Renewable	288:296	arg1	Laboratory					305:314	National Renewable Energy Laboratory	279:314	National Renewable Energy Laboratory	279:314	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	3	32	theme	Raman	529:533	arg1	intensity					542:550	Raman signal intensity	529:550	Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions	529:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	1	33	theme	young	207:211	arg1	bamboo					236:241	young Neosinocalamus affinis bamboo	207:241	young Neosinocalamus affinis bamboo	207:241	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	0	34	theme	Hemicelluloses	36:49	arg1	Structure					51:59	Hemicelluloses Structure	36:59	Hemicelluloses Structure	36:59	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	35	theme	Energy	298:303	arg1	Laboratory					305:314	National Renewable Energy Laboratory	279:314	National Renewable Energy Laboratory	279:314	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	2	36	theme	lignin	422:427	arg1	content					429:435	acid-insoluble lignin content	407:435	acid-insoluble lignin content	407:435	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	3	37	theme	compound	602:609	arg1	regions					626:632	the compound middle lamella regions	598:632	the compound middle lamella regions	598:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	1	38	theme	Neosinocalamus	213:226	arg1	bamboo					236:241	young Neosinocalamus affinis bamboo	207:241	young Neosinocalamus affinis bamboo	207:241	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	2	39	theme	bamboo	488:493	arg1	age					495:497	the increasing bamboo age	473:497	the increasing bamboo age	473:497	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	1	40	theme	Laboratory	305:314	arg1	standard					267:274	standard	267:274	standard	267:274	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	3	41	theme	similar	643:649	arg1	trend					660:664	a similar increase trend	641:664	a similar increase trend	641:664	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	2	42	theme	inverse	447:453	arg1	relationship					455:466	an inverse relationship	444:466	an inverse relationship with the increasing bamboo age	444:497	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	1	43	theme	affinis	228:234	arg1	bamboo					236:241	young Neosinocalamus affinis bamboo	207:241	young Neosinocalamus affinis bamboo	207:241	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	2	44	theme	increasing	477:486	arg1	age					495:497	the increasing bamboo age	473:497	the increasing bamboo age	473:497	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	3	45	theme	increase	651:658	arg1	trend					660:664	a similar increase trend	641:664	a similar increase trend	641:664	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	4	46	theme	affinis	837:843	arg1	culms					852:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	0	47	theme	Structure	51:59	arg1	Changes					0:6	Changes	0:6	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.	0:119	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	1	48	theme	confocal	320:327	arg1	microscopy					335:344	confocal Raman microscopy	320:344	confocal Raman microscopy	320:344	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	1	49	used	used	247:250	arg2	we					244:245	we	244:245	we	244:245	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	4	50	theme	Neosinocalamus	822:835	arg1	culms					852:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	12-month-old Neosinocalamus affinis bamboo culms	809:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	4	51	theme	hemicellulosic	708:721	arg1	fractions					723:731	eight hemicellulosic fractions	702:731	eight hemicellulosic fractions	702:731	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	1	52	theme	Raman	329:333	arg1	microscopy					335:344	confocal Raman microscopy	320:344	confocal Raman microscopy	320:344	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	3	53	theme	middle	611:616	arg1	regions					626:632	the compound middle lamella regions	598:632	the compound middle lamella regions	598:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	2	54	with	relationship	455:466	arg1	age					495:497	the increasing bamboo age	473:497	the increasing bamboo age	473:497	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	1	55	theme	bamboo	236:241	arg1	analysis					170:177	chemical composition analysis	149:177	chemical composition analysis	149:177	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	1	55	theme	bamboo	236:241	arg1	distribution					191:202	spatial distribution	183:202	spatial distribution	183:202	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	4	56	theme	2-	793:794	arg1	holocellulose					772:784	holocellulose	772:784	holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms	772:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	4	57	theme	4-	797:798	arg1	holocellulose					772:784	holocellulose	772:784	holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms	772:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	3	58	theme	Raman	500:504	arg1	analysis					506:513	Raman analysis	500:513	Raman analysis	500:513	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	6	59	theme	DMSO-soluble	1020:1031	arg1	hemicelluloses					1033:1046	DMSO-soluble hemicelluloses	1020:1046	DMSO-soluble hemicelluloses of young bamboo culms	1020:1068	FT-IR and NMR analyses revealed that DMSO-soluble hemicelluloses of young bamboo culms are mainly composed of highly substituted xylans and β-d-glucans.					
30102859	0	60	theme	Aged	76:79	arg1	Culms					114:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Differently Aged Bamboo ( Neosinocalamus affinis) Culms	64:118	Changes of Chemical Composition and Hemicelluloses Structure in Differently Aged Bamboo ( Neosinocalamus affinis) Culms.					
30102859	2	61	theme	acid-insoluble	407:420	arg1	content					429:435	acid-insoluble lignin content	407:435	acid-insoluble lignin content	407:435	It was found that the acid-soluble lignin and acid-insoluble lignin content showed an inverse relationship with the increasing bamboo age.					
30102859	3	62	theme	lamella	618:624	arg1	regions					626:632	the compound middle lamella regions	598:632	the compound middle lamella regions	598:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	3	63	theme	signal	535:540	arg1	intensity					542:550	Raman signal intensity	529:550	Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions	529:632	Raman analysis revealed that Raman signal intensity of lignin in both the secondary cell wall and the compound middle lamella regions showed a similar increase trend with growth of bamboo.					
30102859	1	64	theme	microscopy	335:344	arg1	standard					267:274	standard	267:274	standard	267:274	To study the differences in chemical composition analysis and spatial distribution of young Neosinocalamus affinis bamboo, we used the methods of standard of National Renewable Energy Laboratory and confocal Raman microscopy, respectively.					
30102859	4	65	theme	8-	801:802	arg1	holocellulose					772:784	holocellulose	772:784	holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms	772:856	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
30102859	4	66	dep	2-	793:794	arg1	the					789:791	the	789:791	the	789:791	In addition, eight hemicellulosic fractions were obtained by successively treating holocellulose of the 2-, 4-, 8-, and 12-month-old Neosinocalamus affinis bamboo culms with DMSO and alkaline solution.					
29921300	7	0	theme	fluorescence	1085:1096	arg1	emission					1098:1105	fluorescence emission	1085:1105	fluorescence emission in the visible range	1085:1126	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	3	1	theme	Food-grade	448:457	arg1	E171					459:462	Food-grade E171	448:462	Food-grade E171	448:462	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	13	2	theme	acute	1731:1735	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	0	3	theme	barrier	165:171	arg1	protection					173:182	gut barrier protection	161:182	gut barrier protection	161:182	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	4	4	theme	OECD	762:765	arg1	nanomaterial					777:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	4	5	theme	comprehensive	588:600	arg1	approach					602:609	a comprehensive approach	586:609	a comprehensive approach	586:609	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	13	6	theme	sub-chronic	1740:1750	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	11	7	theme	regular	1502:1508	arg1	"					1518:1518	regular "islands"	1502:1518	regular "islands" located approximately 20 µm above the substratum	1502:1567	The mucus layer was thin and organized into regular "islands" located approximately 20 µm above the substratum.					
29921300	14	8	theme	acid	1872:1875	arg1	profiles					1877:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	8	9	theme	mucoid	1193:1198	arg1	area					1200:1203	the mucoid area	1189:1203	the mucoid area of HT29-MTX cells	1189:1221	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells was visualized in situ.					
29921300	7	10	theme	TiO2	1048:1051	arg1	particles					1053:1061	E171 TiO2 particles	1043:1061	E171 TiO2 particles	1043:1061	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	14	11	theme	short-chain	1854:1864	arg1	profiles					1877:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	6	12	theme	laser	916:920	arg1	microscopy					931:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	5	13	theme	epithelial	878:887	arg1	cells					889:893	HT29-MTX intestinal epithelial cells	858:893	HT29-MTX intestinal epithelial cells	858:893	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	0	14	theme	short-chain	102:112	arg1	synthesis					125:133	short-chain fatty acid synthesis	102:133	short-chain fatty acid synthesis	102:133	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	3	15	theme	gut	491:493	arg1	mucus					482:486	mucus	482:486	mucus	482:486	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	3	15	theme	gut	491:493	arg1	protagonist					503:513	a gut barrier protagonist	489:513	a gut barrier protagonist still poorly explored in food nanotoxicology	489:558	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	1	16	theme	BACKGROUND	185:194	arg1	TiO2					214:217	TiO2	214:217	TiO2	214:217	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	1	16	theme	BACKGROUND	185:194	arg1	dioxide					205:211	BACKGROUND Titanium dioxide	185:211	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	6	17	theme	Time-lapse	896:905	arg1	microscopy					931:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	8	18	theme	cells	1217:1221	arg1	area					1200:1203	the mucoid area	1189:1203	the mucoid area of HT29-MTX cells	1189:1221	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells was visualized in situ.					
29921300	0	19	theme	acid	120:123	arg1	synthesis					125:133	short-chain fatty acid synthesis	102:133	short-chain fatty acid synthesis	102:133	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	1	20	theme	dioxide	205:211	arg1	particles					220:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	1	20	theme	dioxide	205:211	arg1	food					253:256	a food	251:256	a food additive (E171 in the EU)	251:282	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	8	21	theme	E171	1174:1177	arg1	TiO2					1179:1182	E171 TiO2	1174:1182	E171 TiO2	1174:1182	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells was visualized in situ.					
29921300	15	22	theme	barrier	2162:2168	arg1	impairment					2170:2179	a mucus barrier impairment	2154:2179	a mucus barrier impairment	2154:2179	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	15	23	theme	gut	2196:2198	arg1	conditions					2201:2210	"healthy gut" conditions	2187:2210	"healthy gut" conditions	2187:2210	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	12	24	theme	patchy	1657:1662	arg1	structure					1664:1672	this mucus patchy structure	1646:1672	this mucus patchy structure	1646:1672	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	5	25	theme	HT29-MTX	858:865	arg1	cells					889:893	HT29-MTX intestinal epithelial cells	858:893	HT29-MTX intestinal epithelial cells	858:893	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	2	26	theme	fraction	438:445	arg1	presence					413:420	the presence	409:420	the presence of a nano-sized fraction	409:445	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	15	27	theme	impairment	2170:2179	arg1	absence					2143:2149	the absence	2139:2149	the absence of a mucus barrier impairment	2139:2179	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	9	28	theme	TiO2	1272:1275	arg1	particles					1277:1285	TiO2 particles	1272:1285	TiO2 particles	1272:1285	One hour after exposure, TiO2 particles accumulated inside "patchy" regions 20 µm above the substratum.					
29921300	4	29	theme	in	637:638	arg1	interactions					658:669	in vitro and in vivo interactions	637:669	in vitro and in vivo interactions between TiO2 and intestinal mucus	637:703	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	11	30	theme	mucus	1462:1466	arg1	layer					1468:1472	The mucus layer	1458:1472	The mucus layer	1458:1472	The mucus layer was thin and organized into regular "islands" located approximately 20 µm above the substratum.					
29921300	5	31	theme	mucus	837:841	arg1	properties					823:832	E171-trapping properties	809:832	E171-trapping properties of mucus	809:841	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	12	32	theme	food-grade	1602:1611	arg1	particles					1618:1626	food-grade TiO2 particles	1602:1626	food-grade TiO2 particles	1602:1626	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	13	33	theme	relevant	1811:1818	arg1	levels					1829:1834	relevant exposure levels	1811:1834	relevant exposure levels for humans	1811:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	12	34	theme	particles	1618:1626	arg1	trapping					1590:1597	The region-specific trapping	1570:1597	The region-specific trapping of food-grade TiO2 particles	1570:1626	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	10	35	theme	HT29-MTX	1386:1393	arg1	cells					1395:1399	HT29-MTX cells	1386:1399	HT29-MTX cells	1386:1399	The structure of mucus produced by HT29-MTX cells was characterized by MUC5AC immunofluorescence staining.					
29921300	0	36	theme	Food-grade	0:9	arg1	TiO2					11:14	Food-grade TiO2	0:14	Food-grade TiO2	0:14	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	4	37	dep	in	650:651	arg1	vivo					653:656	vivo	653:656	vivo	653:656	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	10	38	theme	immunofluorescence	1429:1446	arg1	staining					1448:1455	MUC5AC immunofluorescence staining	1422:1455	MUC5AC immunofluorescence staining	1422:1455	The structure of mucus produced by HT29-MTX cells was characterized by MUC5AC immunofluorescence staining.					
29921300	13	39	theme	E171	1792:1795	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	2	40	theme	disruption	362:371	arg1	concern					390:396	an increasing concern	376:396	an increasing concern	376:396	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	2	40	theme	disruption	362:371	arg1	risk					342:345	the risk	338:345	the risk of gut barrier disruption	338:371	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	1	41	theme	additive	258:265	arg1	particles					220:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	1	41	theme	additive	258:265	arg1	food					253:256	a food	251:256	a food additive (E171 in the EU)	251:282	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	6	42	theme	particle	1002:1009	arg1	surface					1011:1017	the particle surface	998:1017	the particle surface	998:1017	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	13	43	theme	NM-105	1801:1806	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	2	44	theme	gut	350:352	arg1	disruption					362:371	gut barrier disruption	350:371	gut barrier disruption	350:371	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	7	45	theme	visible	1114:1120	arg1	range					1122:1126	the visible range	1110:1126	the visible range	1110:1126	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	15	46	theme	acid	2104:2107	arg1	synthesis					2109:2117	short-chain fatty acid synthesis	2086:2117	short-chain fatty acid synthesis	2086:2117	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	9	47	dep	hour	1251:1254	arg1	exposure					1262:1269	exposure	1262:1269	exposure	1262:1269	One hour after exposure, TiO2 particles accumulated inside "patchy" regions 20 µm above the substratum.					
29921300	4	48	theme	P25	757:759	arg1	nanomaterial					777:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	15	49	theme	"	2199:2199	arg1	conditions					2201:2210	"healthy gut" conditions	2187:2210	"healthy gut" conditions	2187:2210	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	1	50	dep	additive	258:265	arg1	E171					268:271	E171	268:271	E171 in the EU	268:281	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	15	51	theme	short-chain	2086:2096	arg1	acid					2104:2107	short-chain fatty acid	2086:2107	short-chain fatty acid synthesis	2086:2117	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	4	52	theme	food-grade	719:728	arg1	E171					730:733	food-grade E171	719:733	food-grade E171	719:733	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	9	53	theme	"	1313:1313	arg1	regions					1315:1321	"patchy" regions	1306:1321	"patchy" regions 20 µm above the substratum	1306:1348	One hour after exposure, TiO2 particles accumulated inside "patchy" regions 20 µm above the substratum.					
29921300	13	54	from	levels	1829:1834	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	13	55	theme	oral	1752:1755	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	0	56	theme	gut	161:163	arg1	protection					173:182	gut barrier protection	161:182	gut barrier protection	161:182	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	1	57	from	E171	268:271	arg1	EU					280:281	the EU	276:281	the EU	276:281	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	15	58	theme	mucin	2060:2064	arg1	O-glycosylation					2066:2080	mucin O-glycosylation	2060:2080	mucin O-glycosylation	2060:2080	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	7	59	theme	particles	1053:1061	arg1	irradiation					1028:1038	Near-UV irradiation	1020:1038	Near-UV irradiation of E171 TiO2 particles at 364 nm	1020:1071	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	15	60	theme	intestinal	2014:2023	arg1	mucus					2025:2029	intestinal mucus	2014:2029	intestinal mucus	2014:2029	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	8	61	theme	TiO2	1179:1182	arg1	penetration					1159:1169	The penetration	1155:1169	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells	1155:1221	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells was visualized in situ.					
29921300	7	62	theme	E171	1043:1046	arg1	particles					1053:1061	E171 TiO2 particles	1043:1061	E171 TiO2 particles	1043:1061	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	3	63	theme	barrier	495:501	arg1	mucus					482:486	mucus	482:486	mucus	482:486	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	3	63	theme	barrier	495:501	arg1	protagonist					503:513	a gut barrier protagonist	489:513	a gut barrier protagonist still poorly explored in food nanotoxicology	489:558	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	14	64	theme	fatty	1866:1870	arg1	profiles					1877:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	6	65	theme	scanning	922:929	arg1	microscopy					931:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	4	66	theme	reference	767:775	arg1	nanomaterial					777:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	0	67	theme	fatty	114:118	arg1	synthesis					125:133	short-chain fatty acid synthesis	102:133	short-chain fatty acid synthesis	102:133	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	4	68	theme	Aeroxyde	748:755	arg1	nanomaterial					777:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	14	69	theme	Cecal	1848:1852	arg1	profiles					1877:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles	1848:1884	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	6	70	theme	confocal	907:914	arg1	microscopy					931:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy	896:940	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	15	71	theme	mucus	2156:2160	arg1	impairment					2170:2179	a mucus barrier impairment	2154:2179	a mucus barrier impairment	2154:2179	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	1	72	theme	Titanium	196:203	arg1	TiO2					214:217	TiO2	214:217	TiO2	214:217	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	1	72	theme	Titanium	196:203	arg1	dioxide					205:211	BACKGROUND Titanium dioxide	185:211	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	7	73	from	510 nm	1147:1152	arg1	maximum					1136:1142	a maximum	1134:1142	a maximum at 510 nm	1134:1152	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	8	74	theme	HT29-MTX	1208:1215	arg1	cells					1217:1221	HT29-MTX cells	1208:1221	HT29-MTX cells	1208:1221	The penetration of E171 TiO2 into the mucoid area of HT29-MTX cells was visualized in situ.					
29921300	13	75	theme	TiO2-mediated	1687:1699	arg1	effects					1701:1707	TiO2-mediated effects	1687:1707	TiO2-mediated effects	1687:1707	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	15	76	theme	healthy	2188:2194	arg1	conditions					2201:2210	"healthy gut" conditions	2187:2210	"healthy gut" conditions	2187:2210	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	14	77	theme	O-glycosylation	1900:1914	arg1	patterns					1916:1923	gut mucin O-glycosylation patterns	1890:1923	gut mucin O-glycosylation patterns	1890:1923	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	12	78	theme	mucus	1651:1655	arg1	structure					1664:1672	this mucus patchy structure	1646:1672	this mucus patchy structure	1646:1672	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	4	79	theme	in	650:651	arg1	interactions					658:669	in vitro and in vivo interactions	637:669	in vitro and in vivo interactions between TiO2 and intestinal mucus	637:703	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	15	80	dep	CONCLUSIONS	1972:1982	arg1	trapped					2003:2009	trapped	2003:2009	is trapped by intestinal mucus in vitro	2000:2038	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	15	80	dep	CONCLUSIONS	1972:1982	arg1	affect					2053:2058	affect	2053:2058	affect	2053:2058	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	3	81	theme	food	540:543	arg1	nanotoxicology					545:558	food nanotoxicology	540:558	food nanotoxicology	540:558	Food-grade E171 may interact with mucus, a gut barrier protagonist still poorly explored in food nanotoxicology.					
29921300	5	82	theme	E171-trapping	809:821	arg1	properties					823:832	E171-trapping properties	809:832	E171-trapping properties of mucus	809:841	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	2	83	theme	nano-sized	427:436	arg1	fraction					438:445	a nano-sized fraction	425:445	a nano-sized fraction	425:445	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	7	84	from	emission	1098:1105	arg1	range					1122:1126	the visible range	1110:1126	the visible range	1110:1126	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	10	85	theme	mucus	1368:1372	arg1	structure					1355:1363	The structure	1351:1363	The structure of mucus produced by HT29-MTX cells	1351:1399	The structure of mucus produced by HT29-MTX cells was characterized by MUC5AC immunofluorescence staining.					
29921300	0	86	theme	mucin	76:80	arg1	O-glycosylation					82:96	mucin O-glycosylation	76:96	mucin O-glycosylation	76:96	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	14	87	theme	mucin	1894:1898	arg1	patterns					1916:1923	gut mucin O-glycosylation patterns	1890:1923	gut mucin O-glycosylation patterns	1890:1923	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	4	88	dep	in	637:638	arg1	vitro					640:644	vitro	640:644	vitro	640:644	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	4	89	theme	intestinal	688:697	arg1	mucus					699:703	intestinal mucus	688:703	intestinal mucus	688:703	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	1	90	theme	opacifying	306:315	arg1	properties					317:326	opacifying properties	306:326	opacifying properties	306:326	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	13	91	theme	exposure	1820:1827	arg1	levels					1829:1834	relevant exposure levels	1811:1834	relevant exposure levels for humans	1811:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	5	92	theme	intestinal	867:876	arg1	cells					889:893	HT29-MTX intestinal epithelial cells	858:893	HT29-MTX intestinal epithelial cells	858:893	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	12	93	theme	TiO2	1613:1616	arg1	particles					1618:1626	food-grade TiO2 particles	1602:1626	food-grade TiO2 particles	1602:1626	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	2	94	theme	increasing	379:388	arg1	concern					390:396	an increasing concern	376:396	an increasing concern	376:396	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	2	94	theme	increasing	379:388	arg1	risk					342:345	the risk	338:345	the risk of gut barrier disruption	338:371	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	13	95	theme	food-grade	1781:1790	arg1	E171					1792:1795	food-grade E171	1781:1795	food-grade E171	1781:1795	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
29921300	6	96	theme	surface	1011:1017	arg1	modification					982:993	modification	982:993	modification of the particle surface	982:1017	Time-lapse confocal laser scanning microscopy was performed without labeling to avoid modification of the particle surface.					
29921300	10	97	theme	MUC5AC	1422:1427	arg1	staining					1448:1455	MUC5AC immunofluorescence staining	1422:1455	MUC5AC immunofluorescence staining	1422:1455	The structure of mucus produced by HT29-MTX cells was characterized by MUC5AC immunofluorescence staining.					
29921300	2	98	theme	barrier	354:360	arg1	disruption					362:371	gut barrier disruption	350:371	gut barrier disruption	350:371	However, the risk of gut barrier disruption is an increasing concern because of the presence of a nano-sized fraction.					
29921300	0	99	theme	intestinal	30:39	arg1	mucus					41:45	intestinal mucus	30:45	intestinal mucus	30:45	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	1	100	used	used	243:246	arg2	particles					220:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles	185:228	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	1	100	used	used	243:246	arg2	food					253:256	a food	251:256	a food additive (E171 in the EU)	251:282	BACKGROUND Titanium dioxide (TiO2) particles are commonly used as a food additive (E171 in the EU) for its whitening and opacifying properties.					
29921300	15	101	theme	fatty	2098:2102	arg1	acid					2104:2107	short-chain fatty acid	2086:2107	short-chain fatty acid synthesis	2086:2117	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	12	102	theme	region-specific	1574:1588	arg1	trapping					1590:1597	The region-specific trapping	1570:1597	The region-specific trapping of food-grade TiO2 particles	1570:1626	The region-specific trapping of food-grade TiO2 particles was attributed to this mucus patchy structure.					
29921300	7	103	from	364 nm	1066:1071	arg1	irradiation					1028:1038	Near-UV irradiation	1020:1038	Near-UV irradiation of E171 TiO2 particles at 364 nm	1020:1071	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	15	104	theme	Food-grade	1984:1993	arg1	TiO2					1995:1998	Food-grade TiO2	1984:1998	Food-grade TiO2	1984:1998	CONCLUSIONS Food-grade TiO2 is trapped by intestinal mucus in vitro but does not affect mucin O-glycosylation and short-chain fatty acid synthesis in vivo, suggesting the absence of a mucus barrier impairment under "healthy gut" conditions.					
29921300	4	105	theme	NM-105	740:745	arg1	nanomaterial					777:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	NM-105 (Aeroxyde P25) OECD reference nanomaterial	740:788	To test this hypothesis, a comprehensive approach was performed to evaluate in vitro and in vivo interactions between TiO2 and intestinal mucus, by comparing food-grade E171 with NM-105 (Aeroxyde P25) OECD reference nanomaterial.					
29921300	11	106	theme	located	1520:1526	arg1	"					1518:1518	regular "islands"	1502:1518	regular "islands" located approximately 20 µm above the substratum	1502:1567	The mucus layer was thin and organized into regular "islands" located approximately 20 µm above the substratum.					
29921300	7	107	theme	Near-UV	1020:1026	arg1	irradiation					1028:1038	Near-UV irradiation	1020:1038	Near-UV irradiation of E171 TiO2 particles at 364 nm	1020:1071	Near-UV irradiation of E171 TiO2 particles at 364 nm resulted in fluorescence emission in the visible range, with a maximum at 510 nm.					
29921300	5	108	dep	RESULTS	791:797	arg1	tested					802:807	tested	802:807	tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells	802:893	RESULTS We tested E171-trapping properties of mucus in vitro using HT29-MTX intestinal epithelial cells.					
29921300	11	109	theme	islands	1511:1517	arg1	"					1518:1518	regular "islands"	1502:1518	regular "islands" located approximately 20 µm above the substratum	1502:1567	The mucus layer was thin and organized into regular "islands" located approximately 20 µm above the substratum.					
29921300	0	110	dep	trapped	19:25	arg1	implications					144:155	implications	144:155	implications for gut barrier protection	144:182	Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection.					
29921300	14	111	theme	gut	1890:1892	arg1	patterns					1916:1923	gut mucin O-glycosylation patterns	1890:1923	gut mucin O-glycosylation patterns	1890:1923	Cecal short-chain fatty acid profiles and gut mucin O-glycosylation patterns remained unchanged, irrespective of treatment.					
29921300	13	112	theme	daily	1757:1761	arg1	administration					1763:1776	acute or sub-chronic oral daily administration	1731:1776	acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans	1731:1845	We compared TiO2-mediated effects in vivo in rats after acute or sub-chronic oral daily administration of food-grade E171 and NM-105 at relevant exposure levels for humans.					
30593240	1	0	with	procedure	173:181	arg1	method					236:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	4	1	theme	multiple-reaction	679:695	arg1	mode					714:717	multiple-reaction monitoring (MRM) mode	679:717	multiple-reaction monitoring (MRM) mode	679:717	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	1	2	theme	composition	287:297	arg1	determination					251:263	the determination	247:263	the determination of the monosaccharide composition of 12 polysaccharides	247:319	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	0	3	theme	procedure	95:103	arg1	UPLC-MS/MS					105:114	pre-column derivatization procedure UPLC-MS/MS	69:114	pre-column derivatization procedure UPLC-MS/MS	69:114	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	2	4	theme	Armillaria	392:401	arg1	gallica					403:409	Armillaria gallica	392:409	Armillaria gallica	392:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	5	5	theme	92.30	1032:1036	arg1	%					1037:1037	%	1037:1037	%	1037:1037	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	6	6	located	detected	1110:1117	arg2	mannose					1084:1090	mannose	1084:1090	mannose	1084:1090	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	6	6	located	detected	1110:1117	arg1	samples					1133:1139	A. gallica samples	1122:1139	A. gallica samples	1122:1139	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	6	6	located	detected	1110:1117	arg2	glucose					1097:1103	glucose	1097:1103	glucose	1097:1103	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	6	6	located	detected	1110:1117	arg2	fructose					1074:1081	fructose	1074:1081	fructose	1074:1081	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	6	6	located	detected	1110:1117	arg2	monosaccharides					1050:1064	11 monosaccharides	1047:1064	11 monosaccharides such as fructose, mannose, and glucose	1047:1103	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	3	7	dep	column	497:502	arg1	mm					515:516	2.1 mm×100 mm	504:516	2.1 mm×100 mm	504:516	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	7	dep	column	497:502	arg1	μm					523:524	1.8 μm	519:524	1.8 μm	519:524	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	2	8	from	components	359:368	arg1	gallica					403:409	Armillaria gallica	392:409	Armillaria gallica	392:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	2	9	theme	same	329:332	arg1	time					334:337	the same time	325:337	the same time	325:337	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	5	10	theme	monosaccharides	932:946	arg1	components					948:957	the 12 monosaccharides components	925:957	the 12 monosaccharides components	925:957	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	11	theme	components	948:957	arg1	linear					964:969	linear	964:969	linear	964:969	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	11	theme	components	948:957	arg1	linearity					912:920	the linearity	908:920	the linearity of the 12 monosaccharides components	908:957	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	4	12	theme	electrospray	745:756	arg1	source					775:780	electrospray ionization (ESI) source	745:780	electrospray ionization (ESI) source operated in ionization mode	745:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	6	13	theme	A.	1122:1123	arg1	samples					1133:1139	A. gallica samples	1122:1139	A. gallica samples	1122:1139	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	5	14	from	range	987:991	arg1	linear					964:969	linear	964:969	linear	964:969	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	14	from	range	987:991	arg1	linearity					912:920	the linearity	908:920	the linearity of the 12 monosaccharides components	908:957	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	1	15	theme	polysaccharides	305:319	arg1	composition					287:297	the monosaccharide composition	268:297	the monosaccharide composition of 12 polysaccharides	268:319	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	4	16	located	detected	667:674	arg2	components					651:660	The target components	640:660	The target components	640:660	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	4	16	located	detected	667:674	arg1	mode					714:717	multiple-reaction monitoring (MRM) mode	679:717	multiple-reaction monitoring (MRM) mode	679:717	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	2	17	from	polysaccharides	373:387	arg1	gallica					403:409	Armillaria gallica	392:409	Armillaria gallica	392:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	3	18	theme	95	533:534	arg1	%					535:535	%	535:535	%	535:535	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	19	theme	ZORBAX	468:473	arg1	RRHD					475:478	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	1	20	theme	1-phenyl-3-methyl-5-pyrazolone	188:217	arg1	method					236:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	2	21	from	gallica	403:409	arg1	components					359:368	the monosaccharide components	340:368	the monosaccharide components of polysaccharides in Armillaria gallica	340:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	3	22	theme	%	535:535	arg1	acetonitrile					537:548	95% acetonitrile	533:548	95% acetonitrile (A)	533:552	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	22	theme	%	535:535	arg1	A					551:551	A	551:551	A	551:551	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	23	theme	Plus	488:491	arg1	column					497:502	Plus C₁₈ column	488:502	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	5	24	theme	linear	980:985	arg1	range					987:991	their linear range	974:991	their linear range (R²>0.990)	974:1002	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	24	theme	linear	980:985	arg1	R²>0.990					994:1001	R²>0.990	994:1001	R²>0.990	994:1001	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	2	25	theme	monosaccharide	344:357	arg1	components					359:368	the monosaccharide components	340:368	the monosaccharide components of polysaccharides in Armillaria gallica	340:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	1	26	theme	PMP	220:222	arg1	method					236:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	4	27	theme	ESI	770:772	arg1	source					775:780	electrospray ionization (ESI) source	745:780	electrospray ionization (ESI) source operated in ionization mode	745:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	3	28	theme	ammonium	558:565	arg1	acetonitrile-water					578:595	ammonium acetate-5% acetonitrile-water	558:595	ammonium acetate-5% acetonitrile-water (B)	558:599	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	28	theme	ammonium	558:565	arg1	B					598:598	B	598:598	B	598:598	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	29	with	phase	611:615	arg1	elution					631:637	gradient elution	622:637	gradient elution	622:637	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	4	30	theme	target	644:649	arg1	components					651:660	The target components	640:660	The target components	640:660	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	0	31	theme	composition	28:38	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS	0:114	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	1	32	theme	UPLC-MS/MS	225:234	arg1	method					236:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method	188:241	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	0	33	theme	monosaccharide	13:26	arg1	composition					28:38	monosaccharide composition	13:38	monosaccharide composition of twelve polysaccharides	13:64	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	6	34	theme	gallica	1125:1131	arg1	samples					1133:1139	A. gallica samples	1122:1139	A. gallica samples	1122:1139	11 monosaccharides such as fructose, mannose, and glucose were detected in A. gallica samples.					
30593240	3	35	theme	gradient	622:629	arg1	elution					631:637	gradient elution	622:637	gradient elution	622:637	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	4	36	theme	mass	722:725	arg1	spectrometry					727:738	mass spectrometry	722:738	mass spectrometry with electrospray ionization (ESI) source operated in ionization mode	722:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	5	37	theme	detection	864:872	arg1	method					874:879	the monosaccharides detection method	844:879	the monosaccharides detection method established by UPLC-MS/MS	844:905	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	4	38	theme	monitoring	697:706	arg1	mode					714:717	multiple-reaction monitoring (MRM) mode	679:717	multiple-reaction monitoring (MRM) mode	679:717	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	4	39	theme	ionization	794:803	arg1	mode					805:808	ionization mode	794:808	ionization mode	794:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	5	40	theme	monosaccharides	848:862	arg1	method					874:879	the monosaccharides detection method	844:879	the monosaccharides detection method established by UPLC-MS/MS	844:905	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	3	41	theme	C₁₈	493:495	arg1	column					497:502	Plus C₁₈ column	488:502	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	42	theme	ACQUITY	460:466	arg1	RRHD					475:478	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	4	43	with	spectrometry	727:738	arg1	source					775:780	electrospray ionization (ESI) source	745:780	electrospray ionization (ESI) source operated in ionization mode	745:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	5	44	theme	-105.6	1038:1043	arg1	%					1037:1037	%	1037:1037	%	1037:1037	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	3	45	dep	RRHD	475:478	arg1	Eclipse					480:486	Eclipse	480:486	Eclipse	480:486	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	45	dep	RRHD	475:478	arg1	column					497:502	Plus C₁₈ column	488:502	a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm)	458:525	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	46	theme	acetate-5	567:575	arg1	%					576:576	%	576:576	%	576:576	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	0	47	theme	polysaccharides	50:64	arg1	composition					28:38	monosaccharide composition	13:38	monosaccharide composition of twelve polysaccharides	13:64	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	3	48	theme	%	576:576	arg1	acetonitrile-water					578:595	ammonium acetate-5% acetonitrile-water	558:595	ammonium acetate-5% acetonitrile-water (B)	558:599	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	3	48	theme	%	576:576	arg1	B					598:598	B	598:598	B	598:598	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	2	49	theme	polysaccharides	373:387	arg1	components					359:368	the monosaccharide components	340:368	the monosaccharide components of polysaccharides in Armillaria gallica	340:409	At the same time, the monosaccharide components of polysaccharides in Armillaria gallica were analyzed.					
30593240	5	50	theme	recovery	1013:1020	arg1	rate					1022:1025	the recovery rate	1009:1025	the recovery rate	1009:1025	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	50	theme	recovery	1013:1020	arg1	%					1044:1044	92.30%-105.6%	1032:1044	92.30%-105.6%	1032:1044	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	4	51	theme	ionization	758:767	arg1	source					775:780	electrospray ionization (ESI) source	745:780	electrospray ionization (ESI) source operated in ionization mode	745:808	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	3	52	theme	mobile	604:609	arg1	phase					611:615	mobile phase	604:615	mobile phase with gradient elution	604:637	The separation was performed on a ACQUITY ZORBAX RRHD Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm),using 95% acetonitrile (A) and ammonium acetate-5% acetonitrile-water (B) as mobile phase with gradient elution.					
30593240	4	53	theme	MRM	709:711	arg1	mode					714:717	multiple-reaction monitoring (MRM) mode	679:717	multiple-reaction monitoring (MRM) mode	679:717	The target components were detected in multiple-reaction monitoring (MRM) mode by mass spectrometry with electrospray ionization (ESI) source operated in ionization mode.					
30593240	0	54	theme	derivatization	80:93	arg1	UPLC-MS/MS					105:114	pre-column derivatization procedure UPLC-MS/MS	69:114	pre-column derivatization procedure UPLC-MS/MS	69:114	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	7	55	theme	components	1285:1294	arg1	determination					1253:1265	the determination	1249:1265	the determination of monosaccharide components such as A. gallica	1249:1313	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	1	56	theme	pre-column	147:156	arg1	procedure					173:181	a pre-column derivatization procedure	145:181	a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides	145:319	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	7	57	theme	monosaccharide	1270:1283	arg1	components					1285:1294	monosaccharide components	1270:1294	monosaccharide components such as A. gallica	1270:1313	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	7	57	theme	monosaccharide	1270:1283	arg1	gallica					1307:1313	A. gallica	1304:1313	A. gallica	1304:1313	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	0	58	theme	pre-column	69:78	arg1	UPLC-MS/MS					105:114	pre-column derivatization procedure UPLC-MS/MS	69:114	pre-column derivatization procedure UPLC-MS/MS	69:114	[Analysis of monosaccharide composition of twelve polysaccharides by pre-column derivatization procedure UPLC-MS/MS].					
30593240	7	59	theme	A.	1304:1305	arg1	gallica					1307:1313	A. gallica	1304:1313	A. gallica	1304:1313	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	7	60	dep	robust	1187:1192	arg1	suitable					1236:1243	suitable	1236:1243	suitable	1236:1243	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	7	60	dep	robust	1187:1192	arg1	method					1146:1151	The method	1142:1151	The method established in this experiment	1142:1182	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	7	60	dep	robust	1187:1192	arg1	robust					1187:1192	robust	1187:1192	robust	1187:1192	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	7	60	dep	robust	1187:1192	arg1	reproducible					1202:1213	reproducible	1202:1213	reproducible	1202:1213	The method established in this experiment is robust, highly reproducible and accurate, and is suitable for the determination of monosaccharide components such as A. gallica.					
30593240	1	61	theme	derivatization	158:171	arg1	procedure					173:181	a pre-column derivatization procedure	145:181	a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides	145:319	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30593240	5	62	from	linear	964:969	arg1	range					987:991	their linear range	974:991	their linear range (R²>0.990)	974:1002	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	5	62	from	linear	964:969	arg1	R²>0.990					994:1001	R²>0.990	994:1001	R²>0.990	994:1001	The results showed that based on the monosaccharides detection method established by UPLC-MS/MS, the linearity of the 12 monosaccharides components were linear in their linear range (R²>0.990), and the recovery rate were 92.30%-105.6%.					
30593240	1	63	theme	monosaccharide	272:285	arg1	composition					287:297	the monosaccharide composition	268:297	the monosaccharide composition of 12 polysaccharides	268:319	This study is to establish a pre-column derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) UPLC-MS/MS method for the determination of the monosaccharide composition of 12 polysaccharides.					
30171898	8	0	theme	cellular	1422:1429	arg1	uptake					1431:1436	cellular uptake	1422:1436	cellular uptake	1422:1436	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	1	1	theme	Different	155:163	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	1	theme	Different	155:163	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	2	theme	cell	241:244	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	2	theme	cell	241:244	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	0	3	from	time	10:13	arg1	mucosa					95:100	the nasal mucosa	85:100	the nasal mucosa	85:100	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	5	4	theme	residence	840:848	arg1	time					850:853	their nasal residence time	828:853	their nasal residence time	828:853	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	4	5	theme	anionic	669:675	arg1	Acid					707:710	Poly Lactic co-Glycolic Acid	683:710	Poly Lactic co-Glycolic Acid	683:710	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	5	theme	anionic	669:675	arg1	PLGA					677:680	cationic and anionic PLGA	656:680	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	1	6	theme	delivery	246:253	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	6	theme	delivery	246:253	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	6	7	from	endocytosis	969:979	arg1	cells					992:996	mucosal cells	984:996	mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles	984:1083	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	8	theme	flow	1103:1106	arg1	cytometry					1108:1116	flow cytometry	1103:1116	flow cytometry	1103:1116	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	5	9	theme	live	904:907	arg1	imaging					909:915	in vivo live imaging	896:915	in vivo live imaging	896:915	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	8	10	theme	surface	1353:1359	arg1	charge					1361:1366	the nanoparticles' cationic surface charge	1325:1366	the nanoparticles' cationic surface charge	1325:1366	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	0	11	theme	nasal	89:93	arg1	mucosa					95:100	the nasal mucosa	85:100	the nasal mucosa	85:100	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	4	12	theme	porous	720:725	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	12	theme	porous	720:725	arg1	surface					766:772	cationic surface	757:772	cationic surface with an anionic lipid core	757:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	12	theme	porous	720:725	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	13	theme	PLGA	677:680	arg1	NP					713:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	13	theme	PLGA	677:680	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	14	dep	nanoparticles	505:517	arg1	cationic					561:568	cationic	561:568	cationic	561:568	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	14	dep	nanoparticles	505:517	arg1	anionic					550:556	anionic	550:556	anionic	550:556	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	7	15	theme	greatest	1195:1202	arg1	extent					1204:1209	the greatest extent	1191:1209	the greatest extent	1191:1209	Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized.					
30171898	6	16	theme	labeled	1063:1069	arg1	nanoparticles					1071:1083	labeled nanoparticles	1063:1083	labeled nanoparticles	1063:1083	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	17	theme	mucosal	984:990	arg1	cells					992:996	mucosal cells	984:996	mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles	984:1083	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	4	18	theme	maltodextrin	740:751	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	18	theme	maltodextrin	740:751	arg1	surface					766:772	cationic surface	757:772	cationic surface with an anionic lipid core	757:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	18	theme	maltodextrin	740:751	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	6	19	theme	airway	999:1004	arg1	macrophages					1024:1034	macrophages	1024:1034	macrophages	1024:1034	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	19	theme	airway	999:1004	arg1	cells					1050:1054	dendritic cells	1040:1054	dendritic cells	1040:1054	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	19	theme	airway	999:1004	arg1	cells					1017:1021	airway epithelial cells	999:1021	airway epithelial cells	999:1021	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	4	20	theme	anionic	637:643	arg1	NP					713:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	20	theme	anionic	637:643	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	20	theme	anionic	637:643	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	21	with	surface	766:772	arg1	core					796:799	an anionic lipid core	779:799	an anionic lipid core	779:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	0	22	with	Comparison	103:112	arg1	nanoparticles					140:152	anionic and cationic nanoparticles	119:152	anionic and cationic nanoparticles	119:152	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	3	23	theme	simultaneous	374:385	arg1	comparisons					387:397	only a few simultaneous comparisons	363:397	only a few simultaneous comparisons	363:397	However, only a few simultaneous comparisons have been made and it is therefore difficult to determine the best candidate.					
30171898	8	24	theme	mucosal	1395:1401	arg1	time					1413:1416	mucosal residence time	1395:1416	mucosal residence time	1395:1416	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	1	25	theme	biodegradable	174:186	arg1	nanoparticles					188:200	biodegradable nanoparticles	174:200	biodegradable nanoparticles (NP)	174:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	25	theme	biodegradable	174:186	arg1	NP					203:204	NP	203:204	NP	203:204	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	6	26	dep	In	960:961	arg1	vitro					963:967	vitro	963:967	vitro	963:967	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	5	27	theme	longest	936:942	arg1	time					954:957	the longest residence time	932:957	the longest residence time	932:957	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	2	28	theme	mucosa	315:320	arg1	cells					322:326	mucosa cells	315:326	mucosa cells	315:326	These nanoparticles need to strongly interact with mucosa cells to deliver their payload.					
30171898	1	29	theme	nanoparticles	188:200	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	29	theme	nanoparticles	188:200	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	0	30	theme	Residence	0:8	arg1	time					10:13	Residence time	0:13	Residence time	0:13	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	8	31	theme	residence	1403:1411	arg1	time					1413:1416	mucosal residence time	1395:1416	mucosal residence time	1395:1416	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	4	32	theme	surface	534:540	arg1	charge					542:547	different surface charge	524:547	different surface charge	524:547	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	0	33	theme	anionic	119:125	arg1	nanoparticles					140:152	anionic and cationic nanoparticles	119:152	anionic and cationic nanoparticles	119:152	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	4	34	with	nanoparticles	505:517	arg1	charge					542:547	different surface charge	524:547	different surface charge	524:547	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	7	35	theme	different	1220:1228	arg1	lines					1235:1239	the 3 different cell lines	1214:1239	the 3 different cell lines	1214:1239	Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized.					
30171898	6	36	dep	cells	992:996	arg1	macrophages					1024:1034	macrophages	1024:1034	macrophages	1024:1034	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	36	dep	cells	992:996	arg1	cells					1050:1054	dendritic cells	1040:1054	dendritic cells	1040:1054	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	36	dep	cells	992:996	arg1	cells					1017:1021	airway epithelial cells	999:1021	airway epithelial cells	999:1021	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	37	theme	confocal	1122:1129	arg1	microscopy					1131:1140	confocal microscopy	1122:1140	confocal microscopy	1122:1140	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	4	38	theme	potential	605:613	arg1	vectors					615:621	potential vectors	605:621	potential vectors	605:621	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	0	39	theme	porous	29:34	arg1	nanoparticles					68:80	porous and cationic maltodextrin-based nanoparticles	29:80	porous and cationic maltodextrin-based nanoparticles	29:80	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	5	40	theme	residence	944:952	arg1	time					954:957	the longest residence time	932:957	the longest residence time	932:957	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	0	41	theme	cationic	131:138	arg1	nanoparticles					140:152	anionic and cationic nanoparticles	119:152	anionic and cationic nanoparticles	119:152	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	4	42	theme	cationic	624:631	arg1	NP					713:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	42	theme	cationic	624:631	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	42	theme	cationic	624:631	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	43	dep	surface	766:772	arg1	NPL					802:804	NPL	802:804	NPL	802:804	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	44	theme	nanoparticles	505:517	arg1	types					496:500	5 types	494:500	5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	494:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	45	with	compositions	589:600	arg1	charge					542:547	different surface charge	524:547	different surface charge	524:547	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	8	46	theme	mucosal	1503:1509	arg1	cells					1511:1515	airway mucosal cells	1496:1515	airway mucosal cells	1496:1515	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	0	47	theme	cationic	40:47	arg1	nanoparticles					68:80	porous and cationic maltodextrin-based nanoparticles	29:80	porous and cationic maltodextrin-based nanoparticles	29:80	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	4	48	dep	types	496:500	arg1	NP					713:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	48	dep	types	496:500	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	48	dep	types	496:500	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	8	49	theme	best	1463:1466	arg1	NPL					1451:1453	the NPL	1447:1453	the NPL	1447:1453	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	8	49	theme	best	1463:1466	arg1	candidates					1468:1477	the best candidates	1459:1477	the best candidates to interact with airway mucosal cells	1459:1515	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	4	50	theme	lipid	790:794	arg1	core					796:799	an anionic lipid core	779:799	an anionic lipid core	779:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	6	51	theme	dendritic	1040:1048	arg1	cells					1050:1054	dendritic cells	1040:1054	dendritic cells	1040:1054	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	51	theme	dendritic	1040:1048	arg1	cells					1017:1021	airway epithelial cells	999:1021	airway epithelial cells	999:1021	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	4	52	theme	Poly	683:686	arg1	Acid					707:710	Poly Lactic co-Glycolic Acid	683:710	Poly Lactic co-Glycolic Acid	683:710	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	52	theme	Poly	683:686	arg1	PLGA					677:680	cationic and anionic PLGA	656:680	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	53	theme	various	575:581	arg1	compositions					589:600	various inner compositions	575:600	various inner compositions	575:600	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	54	theme	different	524:532	arg1	charge					542:547	different surface charge	524:547	different surface charge	524:547	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	8	55	theme	airway	1496:1501	arg1	cells					1511:1515	airway mucosal cells	1496:1515	airway mucosal cells	1496:1515	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	4	56	theme	anionic	782:788	arg1	core					796:799	an anionic lipid core	779:799	an anionic lipid core	779:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	7	57	theme	endocytosis	1249:1259	arg1	mechanisms					1261:1270	the endocytosis mechanisms	1245:1270	the endocytosis mechanisms	1245:1270	Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized.					
30171898	5	58	theme	nasal	861:865	arg1	administration					867:880	nasal administration	861:880	nasal administration in mice using in vivo live imaging	861:915	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	4	59	theme	cationic	757:764	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	59	theme	cationic	757:764	arg1	surface					766:772	cationic surface	757:772	cationic surface with an anionic lipid core	757:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	5	60	from	administration	867:880	arg1	mice					885:888	mice	885:888	mice using in vivo live imaging	885:915	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	8	61	theme	cationic	1344:1351	arg1	charge					1361:1366	the nanoparticles' cationic surface charge	1325:1366	the nanoparticles' cationic surface charge	1325:1366	Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.					
30171898	6	62	theme	In	960:961	arg1	endocytosis					969:979	In vitro endocytosis	960:979	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles	960:1083	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	3	63	theme	best	461:464	arg1	candidate					466:474	the best candidate	457:474	the best candidate	457:474	However, only a few simultaneous comparisons have been made and it is therefore difficult to determine the best candidate.					
30171898	5	64	theme	in	896:897	arg1	imaging					909:915	in vivo live imaging	896:915	in vivo live imaging	896:915	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	4	65	theme	inner	583:587	arg1	compositions					589:600	various inner compositions	575:600	various inner compositions	575:600	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	66	theme	cationic	731:738	arg1	NP					753:754	porous and cationic maltodextrin NP	720:754	porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	720:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	66	theme	cationic	731:738	arg1	surface					766:772	cationic surface	757:772	cationic surface with an anionic lipid core	757:799	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	66	theme	cationic	731:738	arg1	liposomes					645:653	cationic and anionic liposomes	624:653	cationic and anionic liposomes	624:653	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	5	67	dep	in	896:897	arg1	vivo					899:902	vivo	899:902	vivo	899:902	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	4	68	theme	Lactic	688:693	arg1	Acid					707:710	Poly Lactic co-Glycolic Acid	683:710	Poly Lactic co-Glycolic Acid	683:710	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	68	theme	Lactic	688:693	arg1	PLGA					677:680	cationic and anionic PLGA	656:680	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	69	theme	co-Glycolic	695:705	arg1	Acid					707:710	Poly Lactic co-Glycolic Acid	683:710	Poly Lactic co-Glycolic Acid	683:710	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	69	theme	co-Glycolic	695:705	arg1	PLGA					677:680	cationic and anionic PLGA	656:680	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	0	70	theme	nanoparticles	68:80	arg1	uptake					19:24	uptake	19:24	uptake	19:24	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	0	70	theme	nanoparticles	68:80	arg1	time					10:13	Residence time	0:13	Residence time	0:13	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	7	71	theme	cell	1230:1233	arg1	lines					1235:1239	the 3 different cell lines	1214:1239	the 3 different cell lines	1214:1239	Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized.					
30171898	0	72	dep	time	10:13	arg1	Comparison					103:112	Comparison	103:112	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.	0:153	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	5	73	theme	nasal	834:838	arg1	time					850:853	their nasal residence time	828:853	their nasal residence time	828:853	We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time.					
30171898	6	74	theme	epithelial	1006:1015	arg1	macrophages					1024:1034	macrophages	1024:1034	macrophages	1024:1034	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	74	theme	epithelial	1006:1015	arg1	cells					1050:1054	dendritic cells	1040:1054	dendritic cells	1040:1054	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	6	74	theme	epithelial	1006:1015	arg1	cells					1017:1021	airway epithelial cells	999:1021	airway epithelial cells	999:1021	In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy.					
30171898	1	75	theme	nasal	228:232	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	75	theme	nasal	228:232	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	0	76	theme	maltodextrin-based	49:66	arg1	nanoparticles					68:80	porous and cationic maltodextrin-based nanoparticles	29:80	porous and cationic maltodextrin-based nanoparticles	29:80	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	0	77	from	uptake	19:24	arg1	mucosa					95:100	the nasal mucosa	85:100	the nasal mucosa	85:100	Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.					
30171898	4	78	theme	compositions	589:600	arg1	types					496:500	5 types	494:500	5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL)	494:805	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	79	theme	cationic	656:663	arg1	Acid					707:710	Poly Lactic co-Glycolic Acid	683:710	Poly Lactic co-Glycolic Acid	683:710	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	4	79	theme	cationic	656:663	arg1	PLGA					677:680	cationic and anionic PLGA	656:680	cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP	656:714	Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL).					
30171898	1	80	theme	mucosa	234:239	arg1	systems					255:261	nasal mucosa cell delivery systems	228:261	nasal mucosa cell delivery systems	228:261	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30171898	1	80	theme	mucosa	234:239	arg1	types					165:169	Different types	155:169	Different types of biodegradable nanoparticles (NP)	155:205	Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems.					
30414905	0	0	theme	Astragalus	95:104	arg1	polysaccharides					106:120	Astragalus polysaccharides	95:120	Astragalus polysaccharides	95:120	Multi-fingerprint profiling combined with chemometric methods for investigating the quality of Astragalus polysaccharides.					
30414905	1	1	theme	Inner	352:356	arg1	Mongolia					358:365	Inner Mongolia	352:365	Inner Mongolia (NM)	352:370	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	1	1	theme	Inner	352:356	arg1	NM					368:369	NM	368:369	NM	368:369	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	4	2	from	plots	753:757	arg1	differences					724:734	the main differences	715:734	the main differences from the loading plots	715:757	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	5	3	theme	UPLC/Q-TOF-MS	853:865	arg1	fingerprints					867:878	UPLC/Q-TOF-MS fingerprints	853:878	UPLC/Q-TOF-MS fingerprints	853:878	Moreover, UPLC/Q-TOF-MS fingerprints were established based on the monosaccharide composition of the APS.					
30414905	4	4	theme	Galp	823:826	arg1	signals					812:818	carbonyls and H1 signals	795:818	carbonyls and H1 signals of Galp and β-glucose	795:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	1	5	theme	polysaccharides	169:183	arg1	quality					158:164	the quality	154:164	the quality of polysaccharides	154:183	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	6	6	theme	monosaccharides	970:984	arg1	concentration					953:965	The concentration	949:965	The concentration of monosaccharides and results of cluster analysis	949:1016	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	3	7	contain	had	586:588	arg1	APS					582:584	APS	582:584	APS	582:584	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	3	7	contain	had	586:588	arg2	similarity					598:607	reduced similarity	590:607	reduced similarity	590:607	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	0	8	theme	polysaccharides	106:120	arg1	quality					84:90	the quality	80:90	the quality of Astragalus polysaccharides	80:120	Multi-fingerprint profiling combined with chemometric methods for investigating the quality of Astragalus polysaccharides.					
30414905	4	9	theme	β-glucose	832:840	arg1	signals					812:818	carbonyls and H1 signals	795:818	carbonyls and H1 signals of Galp and β-glucose	795:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	5	10	theme	APS	944:946	arg1	composition					925:935	the monosaccharide composition	906:935	the monosaccharide composition of the APS	906:946	Moreover, UPLC/Q-TOF-MS fingerprints were established based on the monosaccharide composition of the APS.					
30414905	2	11	theme	chemical	490:497	arg1	structure					499:507	the chemical structure	486:507	the chemical structure of the harvested APS	486:528	Additionally, FT-IR and 1H NMR were applied to characterize the chemical structure of the harvested APS.					
30414905	4	12	theme	H1	809:810	arg1	signals					812:818	carbonyls and H1 signals	795:818	carbonyls and H1 signals of Galp and β-glucose	795:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	4	13	theme	loading	745:751	arg1	plots					753:757	the loading plots	741:757	the loading plots	741:757	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	1	14	dep	challenge	131:139	arg1	used					269:272	used	269:272	was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis	265:423	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	3	15	theme	fingerprinting	544:557	arg1	results					559:565	The spectral fingerprinting results	531:565	The spectral fingerprinting results	531:565	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	0	16	theme	Multi-fingerprint	0:16	arg1	profiling					18:26	Multi-fingerprint profiling	0:26	Multi-fingerprint profiling	0:26	Multi-fingerprint profiling combined with chemometric methods for investigating the quality of Astragalus polysaccharides.					
30414905	4	17	theme	signals	812:818	arg1	intensity					782:790	the absorption intensity	767:790	the absorption intensity of carbonyls and H1 signals of Galp and β-glucose	767:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	1	18	with	quality	293:299	arg1	the					392:394	the	392:394	the	392:394	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	5	19	theme	monosaccharide	910:923	arg1	composition					925:935	the monosaccharide composition	906:935	the monosaccharide composition of the APS	906:946	Moreover, UPLC/Q-TOF-MS fingerprints were established based on the monosaccharide composition of the APS.					
30414905	1	20	theme	complex	198:204	arg1	structure					215:223	their complex chemical structure	192:223	their complex chemical structure	192:223	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	7	21	theme	multiple	1117:1124	arg1	method					1138:1143	this multiple fingerprint method	1112:1143	this multiple fingerprint method	1112:1143	Overall, this multiple fingerprint method was stable, comprehensive and valid for monitoring APS quality.					
30414905	4	22	theme	main	719:722	arg1	differences					724:734	the main differences	715:734	the main differences from the loading plots	715:757	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	1	23	theme	chemical	206:213	arg1	structure					215:223	their complex chemical structure	192:223	their complex chemical structure	192:223	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	7	24	theme	fingerprint	1126:1136	arg1	method					1138:1143	this multiple fingerprint method	1112:1143	this multiple fingerprint method	1112:1143	Overall, this multiple fingerprint method was stable, comprehensive and valid for monitoring APS quality.					
30414905	1	25	theme	Astragalus	304:313	arg1	APS					332:334	APS	332:334	APS	332:334	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	1	25	theme	Astragalus	304:313	arg1	polysaccharides					315:329	Astragalus polysaccharides	304:329	Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS)	304:385	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	2	26	theme	APS	526:528	arg1	structure					499:507	the chemical structure	486:507	the chemical structure of the harvested APS	486:528	Additionally, FT-IR and 1H NMR were applied to characterize the chemical structure of the harvested APS.					
30414905	3	27	theme	different	629:637	arg1	origins					639:645	different origins	629:645	different origins	629:645	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	3	28	theme	spectral	535:542	arg1	results					559:565	The spectral fingerprinting results	531:565	The spectral fingerprinting results	531:565	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	1	29	theme	polysaccharides	315:329	arg1	quality					293:299	the quality	289:299	the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis	289:423	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	2	30	theme	harvested	516:524	arg1	APS					526:528	the harvested APS	512:528	the harvested APS	512:528	Additionally, FT-IR and 1H NMR were applied to characterize the chemical structure of the harvested APS.					
30414905	0	31	theme	chemometric	42:52	arg1	methods					54:60	chemometric methods	42:60	chemometric methods for investigating the quality of Astragalus polysaccharides	42:120	Multi-fingerprint profiling combined with chemometric methods for investigating the quality of Astragalus polysaccharides.					
30414905	2	32	theme	1H	450:451	arg1	NMR					453:455	1H NMR	450:455	1H NMR	450:455	Additionally, FT-IR and 1H NMR were applied to characterize the chemical structure of the harvested APS.					
30414905	4	33	theme	absorption	771:780	arg1	intensity					782:790	the absorption intensity	767:790	the absorption intensity of carbonyls and H1 signals of Galp and β-glucose	767:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	1	34	used	used	269:272	arg2	profiling					255:263	multi-fingerprint profiling	237:263	multi-fingerprint profiling	237:263	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	4	35	theme	carbonyls	795:803	arg1	signals					812:818	carbonyls and H1 signals	795:818	carbonyls and H1 signals of Galp and β-glucose	795:840	Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose.					
30414905	6	36	used	used	1072:1075	arg2	indicator					1050:1058	an indicator	1047:1058	an indicator that can be used to distinguish NM and GS	1047:1100	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	6	36	used	used	1072:1075	arg2	GlcA					1033:1036	GlcA	1033:1036	GlcA	1033:1036	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	6	37	theme	cluster	1001:1007	arg1	analysis					1009:1016	cluster analysis	1001:1016	cluster analysis	1001:1016	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	6	38	theme	analysis	1009:1016	arg1	monosaccharides					970:984	monosaccharides	970:984	monosaccharides	970:984	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	6	38	theme	analysis	1009:1016	arg1	results					990:996	results	990:996	results	990:996	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
30414905	1	39	theme	multi-fingerprint	237:253	arg1	profiling					255:263	multi-fingerprint profiling	237:263	multi-fingerprint profiling	237:263	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	1	40	theme	chemometric	404:414	arg1	analysis					416:423	chemometric analysis	404:423	chemometric analysis	404:423	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	3	41	theme	reduced	590:596	arg1	similarity					598:607	reduced similarity	590:607	reduced similarity	590:607	The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins.					
30414905	7	42	theme	APS	1196:1198	arg1	quality					1200:1206	APS quality	1196:1206	APS quality	1196:1206	Overall, this multiple fingerprint method was stable, comprehensive and valid for monitoring APS quality.					
30414905	1	43	dep	the	392:394	arg1	help					396:399	help	396:399	help	396:399	It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis.					
30414905	6	44	theme	results	990:996	arg1	concentration					953:965	The concentration	949:965	The concentration of monosaccharides and results of cluster analysis	949:1016	The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS.					
31158451	4	0	theme	older	697:701	arg1	women					722:726	older community-dwelling women	697:726	109 older community-dwelling women (65-70 yrs)	693:738	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	0	theme	older	697:701	arg1	65-70 yrs					729:737	65-70 yrs	729:737	65-70 yrs	729:737	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	9	1	theme	lower	1248:1252	arg1	level					1254:1258	a 12% lower level	1242:1258	a 12% lower level of NA3 (peak 8) and NA4 (peak 10)	1242:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	1	2	theme	proteins	168:175	arg1	function					156:163	the function	152:163	the function of proteins	152:175	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	9	3	theme	NA3	1263:1265	arg1	level					1254:1258	a 12% lower level	1242:1258	a 12% lower level of NA3 (peak 8) and NA4 (peak 10)	1242:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	3	theme	NA3	1263:1265	arg1	%					1236:1236	%	1236:1236	%	1236:1236	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	3	theme	NA3	1263:1265	arg1	NA4					1280:1282	NA4	1280:1282	NA4 (peak 10)	1280:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	3	theme	NA3	1263:1265	arg1	NA3					1263:1265	NA3	1263:1265	NA3 (peak 8)	1263:1274	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	3	theme	NA3	1263:1265	arg1	peak					1268:1271	peak 8	1268:1273	peak 8	1268:1273	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	3	theme	NA3	1263:1265	arg1	peak					1285:1288	peak 10	1285:1291	peak 10	1285:1291	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	3	4	theme	different	508:516	arg1	levels					521:526	different PA levels	508:526	different PA levels	508:526	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	8	5	theme	lower	1011:1015	arg1	level					1017:1021	lower level	1011:1021	lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3)	1011:1079	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	9	6	theme	PA	1199:1200	arg1	guideline					1202:1210	the PA guideline	1195:1210	the PA guideline of time in MVPA	1195:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	6	7	theme	Moderate-to-vigorous	813:832	arg1	MVPA					838:841	MVPA	838:841	MVPA	838:841	Moderate-to-vigorous PA (MVPA) was assessed objectively using accelerometry.					
31158451	6	7	theme	Moderate-to-vigorous	813:832	arg1	PA					834:835	Moderate-to-vigorous PA	813:835	Moderate-to-vigorous PA (MVPA)	813:842	Moderate-to-vigorous PA (MVPA) was assessed objectively using accelerometry.					
31158451	2	8	theme	N-glycan	303:310	arg1	profile					312:318	a specific N-glycan profile	292:318	a specific N-glycan profile	292:318	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	13	9	theme	disease	1798:1804	arg1	prevention					1806:1815	disease prevention	1798:1815	disease prevention	1798:1815	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	4	10	theme	metabolic	595:603	arg1	MetS					615:618	MetS	615:618	MetS	615:618	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	10	theme	metabolic	595:603	arg1	syndrome					605:612	the metabolic syndrome	591:612	the metabolic syndrome (MetS)	591:619	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	0	11	theme	aging	91:95	arg1	biomarkers					77:86	N-glycomic biomarkers	66:86	N-glycomic biomarkers of aging	66:95	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	0	11	theme	aging	91:95	arg1	inactivity					35:44	physical inactivity	26:44	physical inactivity	26:44	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	0	11	theme	aging	91:95	arg1	risk					57:60	metabolic risk	47:60	metabolic risk	47:60	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	10	12	from	spent	1416:1420	arg1	PA					1425:1426	PA	1425:1426	PA	1425:1426	Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans.					
31158451	2	13	theme	specific	294:301	arg1	profile					312:318	a specific N-glycan profile	292:318	a specific N-glycan profile	292:318	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	8	14	theme	NA3F	993:996	arg1	levels					963:968	levels	963:968	levels of NGA2FB (peak 2) and NA3F (peak 9)	963:1005	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	2	15	theme	metabolic	375:383	arg1	profile					390:396	metabolic risk profile	375:396	metabolic risk profile	375:396	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	13	16	theme	different	1778:1786	arg1	stages					1788:1793	different stages	1778:1793	different stages of disease prevention	1778:1815	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	3	17	theme	older	491:495	arg1	women					497:501	older women	491:501	older women with different PA levels and metabolic risk status	491:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	8	18	theme	α	1030:1030	arg1	level					1017:1021	lower level	1011:1021	lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3)	1011:1079	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	3	19	theme	N-glycan	471:478	arg1	profile					480:486	serum N-glycan profile	465:486	serum N-glycan profile in older women with different PA levels and metabolic risk status	465:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	3	20	theme	study	446:450	arg1	aim					427:429	The aim	423:429	The aim of the present study	423:450	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	10	21	theme	time	1411:1414	arg1	spent					1416:1420	time spent	1411:1420	time spent in PA below the MVPA threshold	1411:1451	Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans.					
31158451	4	22	dep	MATERIALS	555:563	arg1	Components					577:586	Components	577:586	Components	577:586	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	11	23	theme	older	1567:1571	arg1	adults					1573:1578	older adults	1567:1578	older adults	1567:1578	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	3	24	from	profile	480:486	arg1	women					497:501	older women	491:501	older women with different PA levels and metabolic risk status	491:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	3	25	theme	metabolic	532:540	arg1	status					547:552	metabolic risk status	532:552	metabolic risk status	532:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	10	26	theme	MVPA	1438:1441	arg1	threshold					1443:1451	the MVPA threshold	1434:1451	the MVPA threshold	1434:1451	Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans.					
31158451	9	27	from	time	1215:1218	arg1	MVPA					1223:1226	MVPA	1223:1226	MVPA	1223:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	28	from	MVPA	1223:1226	arg1	guideline					1202:1210	the PA guideline	1195:1210	the PA guideline of time in MVPA	1195:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	29	dep	active	1332:1337	arg1	p < 0.05					1385:1392	p < 0.05	1385:1392	p < 0.05	1385:1392	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	12	30	theme	proposed	1623:1630	arg1	effect					1632:1637	This proposed effect	1618:1637	This proposed effect on N-glycans	1618:1650	This proposed effect on N-glycans requires engagement in MVPA.					
31158451	9	31	theme	NA4	1280:1282	arg1	level					1254:1258	a 12% lower level	1242:1258	a 12% lower level of NA3 (peak 8) and NA4 (peak 10)	1242:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	31	theme	NA4	1280:1282	arg1	%					1236:1236	%	1236:1236	%	1236:1236	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	31	theme	NA4	1280:1282	arg1	NA4					1280:1282	NA4	1280:1282	NA4 (peak 10)	1280:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	31	theme	NA4	1280:1282	arg1	NA3					1263:1265	NA3	1263:1265	NA3 (peak 8)	1263:1274	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	31	theme	NA4	1280:1282	arg1	peak					1268:1271	peak 8	1268:1273	peak 8	1268:1273	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	31	theme	NA4	1280:1282	arg1	peak					1285:1288	peak 10	1285:1291	peak 10	1285:1291	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	0	32	theme	Detrimental	0:10	arg1	links					12:16	Detrimental links	0:16	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging	0:95	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	3	33	with	women	497:501	arg1	status					547:552	metabolic risk status	532:552	metabolic risk status	532:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	3	33	with	women	497:501	arg1	levels					521:526	different PA levels	508:526	different PA levels	508:526	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	13	34	theme	PA	1741:1742	arg1	guidelines					1744:1753	PA guidelines	1741:1753	PA guidelines	1741:1753	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	3	35	theme	present	438:444	arg1	study					446:450	the present study	434:450	the present study	434:450	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	0	36	theme	physical	26:33	arg1	inactivity					35:44	physical inactivity	26:44	physical inactivity	26:44	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	11	37	theme	metabolic	1595:1603	arg1	status					1610:1615	metabolic risk status	1595:1615	metabolic risk status	1595:1615	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	1	38	gly	glycosylation	128:140	arg1	abnormalities					233:245	age-related metabolic abnormalities	211:245	age-related metabolic abnormalities	211:245	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	1	39	theme	BACKGROUND	98:107	arg1	glycosylation					128:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	1	40	theme	age-related	211:221	arg1	abnormalities					233:245	age-related metabolic abnormalities	211:245	age-related metabolic abnormalities	211:245	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	12	41	from	engagement	1661:1670	arg1	MVPA					1675:1678	MVPA	1675:1678	MVPA	1675:1678	This proposed effect on N-glycans requires engagement in MVPA.					
31158451	9	42	contain	had	1228:1230	arg2	peak					1268:1271	peak 8	1268:1273	peak 8	1268:1273	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg2	peak					1285:1288	peak 10	1285:1291	peak 10	1285:1291	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg2	%					1236:1236	%	1236:1236	%	1236:1236	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg2	level					1254:1258	a 12% lower level	1242:1258	a 12% lower level of NA3 (peak 8) and NA4 (peak 10)	1242:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg1	women					1177:1181	women	1177:1181	women adhering to the PA guideline of time in MVPA	1177:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg2	NA3					1263:1265	NA3	1263:1265	NA3 (peak 8)	1263:1274	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	42	contain	had	1228:1230	arg2	NA4					1280:1282	NA4	1280:1282	NA4 (peak 10)	1280:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	11	43	theme	CONCLUSION	1482:1491	arg1	links					1499:1503	CONCLUSION Novel links	1482:1503	CONCLUSION Novel links between PA behaviors and N-glycan profile	1482:1545	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	1	44	theme	N-linked	109:116	arg1	glycosylation					128:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	1	45	theme	metabolic	223:231	arg1	abnormalities					233:245	age-related metabolic abnormalities	211:245	age-related metabolic abnormalities	211:245	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	5	46	theme	unique	772:777	arg1	structure					788:796	a unique N-glycan structure	770:796	a unique N-glycan structure	770:796	Ten peaks, each representing a unique N-glycan structure were detected.					
31158451	9	47	theme	10	1234:1235	arg1	NA4					1280:1282	NA4	1280:1282	NA4 (peak 10)	1280:1292	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	47	theme	10	1234:1235	arg1	peak					1285:1288	peak 10	1285:1291	peak 10	1285:1291	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	47	theme	10	1234:1235	arg1	peak					1268:1271	peak 8	1268:1273	peak 8	1268:1273	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	47	theme	10	1234:1235	arg1	NA3					1263:1265	NA3	1263:1265	NA3 (peak 8)	1263:1274	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	9	47	theme	10	1234:1235	arg1	%					1236:1236	%	1236:1236	%	1236:1236	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	12	48	from	effect	1632:1637	arg1	N-glycans					1642:1650	N-glycans	1642:1650	N-glycans	1642:1650	This proposed effect on N-glycans requires engagement in MVPA.					
31158451	11	49	theme	Novel	1493:1497	arg1	links					1499:1503	CONCLUSION Novel links	1482:1503	CONCLUSION Novel links between PA behaviors and N-glycan profile	1482:1545	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	1	50	theme	abnormalities	233:245	arg1	development					196:206	development	196:206	development of age-related metabolic abnormalities	196:245	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	4	51	theme	DSA-FACE	656:663	arg1	technology					665:674	DSA-FACE technology	656:674	DSA-FACE technology	656:674	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	5	52	theme	N-glycan	779:786	arg1	structure					788:796	a unique N-glycan structure	770:796	a unique N-glycan structure	770:796	Ten peaks, each representing a unique N-glycan structure were detected.					
31158451	9	53	from	guideline	1202:1210	arg1	MVPA					1223:1226	MVPA	1223:1226	MVPA	1223:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	13	54	theme	prevention	1806:1815	arg1	stages					1788:1793	different stages	1778:1793	different stages of disease prevention	1778:1815	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	8	55	theme	healthier	1136:1144	arg1	p < 0.05					1153:1160	p < 0.05	1153:1160	p < 0.05	1153:1160	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	8	55	theme	healthier	1136:1144	arg1	peers					1146:1150	their healthier peers	1130:1150	their healthier peers (p < 0.05)	1130:1161	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	2	56	theme	physical	256:263	arg1	activity					265:272	physical activity	256:272	physical activity (PA)	256:277	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	2	56	theme	physical	256:263	arg1	PA					275:276	PA	275:276	PA	275:276	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	9	57	theme	time	1215:1218	arg1	guideline					1202:1210	the PA guideline	1195:1210	the PA guideline of time in MVPA	1195:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	1	58	theme	enzymatic	118:126	arg1	glycosylation					128:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	0	59	theme	metabolic	47:55	arg1	risk					57:60	metabolic risk	47:60	metabolic risk	47:60	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	11	60	theme	risk	1605:1608	arg1	status					1610:1615	metabolic risk status	1595:1615	metabolic risk status	1595:1615	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	8	61	theme	NG1	1061:1063	arg1	α					1030:1030	the α	1026:1030	the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3)	1026:1079	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	8	61	theme	NG1	1061:1063	arg1	A2F					1067:1069	NG1(6)A2F	1061:1069	NG1(6)A2F	1061:1069	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	11	62	theme	PA	1513:1514	arg1	behaviors					1516:1524	PA behaviors	1513:1524	PA behaviors	1513:1524	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31158451	8	63	with	women	1102:1106	arg1	MetS					1113:1116	MetS	1113:1116	MetS	1113:1116	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	4	64	theme	community-dwelling	703:720	arg1	women					722:726	older community-dwelling women	697:726	109 older community-dwelling women (65-70 yrs)	693:738	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	64	theme	community-dwelling	703:720	arg1	65-70 yrs					729:737	65-70 yrs	729:737	65-70 yrs	729:737	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	13	65	theme	health	1702:1707	arg1	efforts					1709:1715	public health efforts	1695:1715	public health efforts	1695:1715	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	10	66	attach	linked	1461:1466	arg2	spent					1416:1420	time spent	1411:1420	time spent in PA below the MVPA threshold	1411:1451	Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans.					
31158451	10	66	attach	linked	1461:1466	arg1	N-glycans					1471:1479	N-glycans	1471:1479	N-glycans	1471:1479	Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans.					
31158451	13	67	theme	older	1758:1762	arg1	adults					1764:1769	older adults	1758:1769	older adults	1758:1769	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	3	68	theme	risk	542:545	arg1	status					547:552	metabolic risk status	532:552	metabolic risk status	532:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	8	69	theme	NGA2FB	973:978	arg1	levels					963:968	levels	963:968	levels of NGA2FB (peak 2) and NA3F (peak 9)	963:1005	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	2	70	theme	detrimental	335:345	arg1	links					347:351	detrimental links	335:351	detrimental links between N-glycans and metabolic risk profile	335:396	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	2	71	attach	linked	282:287	arg2	PA					275:276	PA	275:276	PA	275:276	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	2	71	attach	linked	282:287	arg1	profile					312:318	a specific N-glycan profile	292:318	a specific N-glycan profile	292:318	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	2	71	attach	linked	282:287	arg2	activity					265:272	physical activity	256:272	physical activity (PA)	256:277	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	0	72	theme	N-glycomic	66:75	arg1	biomarkers					77:86	N-glycomic biomarkers	66:86	N-glycomic biomarkers of aging	66:95	Detrimental links between physical inactivity, metabolic risk and N-glycomic biomarkers of aging.					
31158451	13	73	theme	public	1695:1700	arg1	efforts					1709:1715	public health efforts	1695:1715	public health efforts	1695:1715	This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.					
31158451	3	74	theme	serum	465:469	arg1	profile					480:486	serum N-glycan profile	465:486	serum N-glycan profile in older women with different PA levels and metabolic risk status	465:552	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	3	75	theme	PA	518:519	arg1	levels					521:526	different PA levels	508:526	different PA levels	508:526	The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status.					
31158451	1	76	link	N-linked	109:116	arg1	glycosylation					128:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation	98:140	BACKGROUND N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities.					
31158451	9	77	theme	adhering	1183:1190	arg1	women					1177:1181	women	1177:1181	women adhering to the PA guideline of time in MVPA	1177:1226	Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05).					
31158451	4	78	theme	serum	625:629	arg1	N-glycans					631:639	serum N-glycans	625:639	serum N-glycans	625:639	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	79	theme	syndrome	605:612	arg1	N-glycans					631:639	serum N-glycans	625:639	serum N-glycans	625:639	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	79	theme	syndrome	605:612	arg1	MATERIALS					555:563	MATERIALS	555:563	MATERIALS	555:563	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	4	79	theme	syndrome	605:612	arg1	METHODS					569:575	METHODS	569:575	METHODS	569:575	MATERIALS AND METHODS Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs).					
31158451	2	80	theme	risk	385:388	arg1	profile					390:396	metabolic risk profile	375:396	metabolic risk profile	375:396	Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored.					
31158451	8	81	theme	monogalactosylated	1041:1058	arg1	α					1030:1030	the α	1026:1030	the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3)	1026:1079	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	8	81	theme	monogalactosylated	1041:1058	arg1	A2F					1067:1069	NG1(6)A2F	1061:1069	NG1(6)A2F	1061:1069	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	8	81	theme	monogalactosylated	1041:1058	arg1	peak					1073:1076	peak 3	1073:1078	peak 3	1073:1078	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	8	81	theme	monogalactosylated	1041:1058	arg1	1,6					1032:1034	1,6	1032:1034	1,6	1032:1034	RESULTS Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05).					
31158451	11	82	theme	N-glycan	1530:1537	arg1	profile					1539:1545	N-glycan profile	1530:1545	N-glycan profile	1530:1545	CONCLUSION Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status.					
31220495	0	0	theme	template	111:118	arg1	method					120:125	bi-molecular template method	98:125	bi-molecular template method	98:125	A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.					
31220495	8	1	theme	collagenase	1183:1193	arg1	resistance					1195:1204	collagenase resistance	1183:1204	collagenase resistance	1183:1204	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	7	2	theme	growth	1006:1011	arg1	rate					1013:1016	The high relative growth rate	988:1016	The high relative growth rate of wild-type mouse embryonic fibroblasts cells	988:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	2	3	theme	Col-CMC/HA	416:425	arg1	composites					427:436	Col-CMC/HA composites	416:436	Col-CMC/HA composites	416:436	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	1	4	theme	biomimetic	289:298	arg1	template					300:307	a biomimetic template	287:307	a biomimetic template of Col and CMC protein-polysaccharide bi-molecules	287:358	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	0	5	theme	bi-molecular	98:109	arg1	method					120:125	bi-molecular template method	98:125	bi-molecular template method	98:125	A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.					
31220495	9	6	theme	tunable	1463:1469	arg1	properties					1471:1480	tunable properties	1463:1480	tunable properties	1463:1480	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	1	7	theme	porous	175:180	arg1	composite					243:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	8	8	theme	initial	1261:1267	arg1	weight					1269:1274	initial weight left	1261:1279	initial weight left at the ratio of Col to CMC of 1:9	1261:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	9	9	theme	safe	1422:1425	arg1	method					1427:1432	a rational and safe method	1407:1432	a rational and safe method to prepare biomaterials with tunable properties	1407:1480	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	9	9	theme	safe	1422:1425	arg1	composite					1361:1369	the Col-CMC/HA composite	1346:1369	the Col-CMC/HA composite by bi-molecular template method	1346:1401	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	7	10	theme	high	992:995	arg1	rate					1013:1016	The high relative growth rate	988:1016	The high relative growth rate of wild-type mouse embryonic fibroblasts cells	988:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	3	11	theme	porous	600:605	arg1	structure					607:615	the composite presented 3D porous structure	573:615	the composite presented 3D porous structure with pore size from 100 μm to 300 μm	573:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	4	12	theme	%	719:719	arg1	range					704:708	the range	700:708	the range of 71%-85%	700:719	The porosities of composites were located at the range of 71%-85%.					
31220495	4	13	theme	composites	673:682	arg1	located					689:695	located	689:695	located	689:695	The porosities of composites were located at the range of 71%-85%.					
31220495	4	13	theme	composites	673:682	arg1	porosities					659:668	The porosities	655:668	The porosities of composites	655:682	The porosities of composites were located at the range of 71%-85%.					
31220495	8	14	from	%	1256:1256	arg1	ratio					1288:1292	the ratio	1284:1292	the ratio of Col to CMC of 1:9	1284:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	3	15	with	structure	607:615	arg1	size					627:630	pore size	622:630	pore size from 100 μm to 300 μm	622:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	3	16	theme	presented	587:595	arg1	structure					607:615	the composite presented 3D porous structure	573:615	the composite presented 3D porous structure with pore size from 100 μm to 300 μm	573:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	1	17	theme	Col	312:314	arg1	bi-molecules					347:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	8	18	theme	Col	1297:1299	arg1	ratio					1288:1292	the ratio	1284:1292	the ratio of Col to CMC of 1:9	1284:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	8	19	theme	left	1276:1279	arg1	weight					1269:1274	initial weight left	1261:1279	initial weight left at the ratio of Col to CMC of 1:9	1261:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	1	20	theme	collagen-carboxymethyl	182:203	arg1	composite					243:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	3	21	theme	pore	622:625	arg1	size					627:630	pore size	622:630	pore size from 100 μm to 300 μm	622:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	1	22	theme	cellulose/hydroxyapatite	205:228	arg1	composite					243:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	0	23	theme	porous	2:7	arg1	collagen-carboxymethyl					9:30	A porous collagen-carboxymethyl	0:30	A porous collagen-carboxymethyl	0:30	A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.					
31220495	9	24	theme	Col-CMC/HA	1350:1359	arg1	method					1427:1432	a rational and safe method	1407:1432	a rational and safe method to prepare biomaterials with tunable properties	1407:1480	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	9	24	theme	Col-CMC/HA	1350:1359	arg1	composite					1361:1369	the Col-CMC/HA composite	1346:1369	the Col-CMC/HA composite by bi-molecular template method	1346:1401	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	8	25	theme	weight	1269:1274	arg1	weight					1269:1274	initial weight left	1261:1279	initial weight left at the ratio of Col to CMC of 1:9	1261:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	8	25	theme	weight	1269:1274	arg1	%					1256:1256	about 50%	1248:1256	about 50% of initial weight left at the ratio of Col to CMC of 1:9	1248:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	6	26	from	composite	856:864	arg1	respect					869:875	respect	869:875	respect to physical properties	869:898	The optimized composite in respect to physical properties showed a compressive strength as high as 7.06 MPa, quite close to that of natural bone.					
31220495	7	27	theme	good	1107:1110	arg1	biocompatibility					1112:1127	a good biocompatibility	1105:1127	a good biocompatibility	1105:1127	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	1	28	theme	Col-CMC/HA	231:240	arg1	composite					243:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite	173:251	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	1	29	theme	CMC	320:322	arg1	bi-molecules					347:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	5	30	theme	organic	824:830	arg1	template					832:839	the organic template	820:839	the organic template	820:839	Besides, the compressive strength of composites was highly depended on the ratio of Col to CMC in the organic template.					
31220495	1	31	theme	protein-polysaccharide	324:345	arg1	bi-molecules					347:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Col and CMC protein-polysaccharide bi-molecules	312:358	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	5	32	theme	composites	759:768	arg1	strength					747:754	the compressive strength	731:754	the compressive strength of composites	731:768	Besides, the compressive strength of composites was highly depended on the ratio of Col to CMC in the organic template.					
31220495	6	33	theme	compressive	909:919	arg1	strength					921:928	a compressive strength	907:928	a compressive strength	907:928	The optimized composite in respect to physical properties showed a compressive strength as high as 7.06 MPa, quite close to that of natural bone.					
31220495	3	34	theme	3D	597:598	arg1	structure					607:615	the composite presented 3D porous structure	573:615	the composite presented 3D porous structure with pore size from 100 μm to 300 μm	573:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	3	35	theme	Col-CMC	546:552	arg1	bi-templates					554:565	Col-CMC bi-templates	546:565	Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm	546:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	1	36	theme	bi-molecules	347:358	arg1	template					300:307	a biomimetic template	287:307	a biomimetic template of Col and CMC protein-polysaccharide bi-molecules	287:358	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	4	37	from	range	704:708	arg1	located					689:695	located	689:695	located	689:695	The porosities of composites were located at the range of 71%-85%.					
31220495	4	37	from	range	704:708	arg1	porosities					659:668	The porosities	655:668	The porosities of composites	655:682	The porosities of composites were located at the range of 71%-85%.					
31220495	2	38	dep	morphology	365:374	arg1	The					361:363	The	361:363	The	361:363	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	6	39	theme	natural	974:980	arg1	bone					982:985	natural bone	974:985	natural bone	974:985	The optimized composite in respect to physical properties showed a compressive strength as high as 7.06 MPa, quite close to that of natural bone.					
31220495	9	40	with	biomaterials	1445:1456	arg1	properties					1471:1480	tunable properties	1463:1480	tunable properties	1463:1480	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	8	41	from	good	1175:1178	arg1	resistance					1195:1204	collagenase resistance	1183:1204	collagenase resistance	1183:1204	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	4	42	located	located	689:695	arg1	range					704:708	the range	700:708	the range of 71%-85%	700:719	The porosities of composites were located at the range of 71%-85%.					
31220495	4	42	located	located	689:695	arg2	located					689:695	located	689:695	located	689:695	The porosities of composites were located at the range of 71%-85%.					
31220495	4	42	located	located	689:695	arg2	porosities					659:668	The porosities	655:668	The porosities of composites	655:682	The porosities of composites were located at the range of 71%-85%.					
31220495	7	43	theme	embryonic	1037:1045	arg1	cells					1059:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	4	44	theme	71	713:714	arg1	%					715:715	%	715:715	%	715:715	The porosities of composites were located at the range of 71%-85%.					
31220495	0	45	theme	tissue	76:81	arg1	engineering					83:93	bone tissue engineering	71:93	bone tissue engineering by bi-molecular template method	71:125	A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.					
31220495	8	46	theme	organic-inorganic	1134:1150	arg1	composite					1152:1160	The organic-inorganic composite	1130:1160	The organic-inorganic composite	1130:1160	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	7	47	theme	fibroblasts	1047:1057	arg1	cells					1059:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	8	48	theme	1:9	1311:1313	arg1	CMC					1304:1306	CMC	1304:1306	CMC of 1:9	1304:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	0	49	theme	bone	71:74	arg1	engineering					83:93	bone tissue engineering	71:93	bone tissue engineering by bi-molecular template method	71:125	A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.					
31220495	9	50	theme	bi-molecular	1374:1385	arg1	method					1396:1401	bi-molecular template method	1374:1401	bi-molecular template method	1374:1401	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	3	51	dep	300 μm	647:652	arg1	to					644:645	to	644:645	to	644:645	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	6	52	theme	physical	880:887	arg1	properties					889:898	physical properties	880:898	physical properties	880:898	The optimized composite in respect to physical properties showed a compressive strength as high as 7.06 MPa, quite close to that of natural bone.					
31220495	7	53	theme	cells	1059:1063	arg1	rate					1013:1016	The high relative growth rate	988:1016	The high relative growth rate of wild-type mouse embryonic fibroblasts cells	988:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	7	54	theme	wild-type	1021:1029	arg1	cells					1059:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	2	55	theme	physical	393:400	arg1	properties					402:411	physical properties	393:411	physical properties	393:411	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	9	56	theme	template	1387:1394	arg1	method					1396:1401	bi-molecular template method	1374:1401	bi-molecular template method	1374:1401	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	5	57	theme	compressive	735:745	arg1	strength					747:754	the compressive strength	731:754	the compressive strength of composites	731:768	Besides, the compressive strength of composites was highly depended on the ratio of Col to CMC in the organic template.					
31220495	5	58	from	CMC	813:815	arg1	template					832:839	the organic template	820:839	the organic template	820:839	Besides, the compressive strength of composites was highly depended on the ratio of Col to CMC in the organic template.					
31220495	6	59	theme	optimized	846:854	arg1	composite					856:864	The optimized composite	842:864	The optimized composite in respect to physical properties	842:898	The optimized composite in respect to physical properties showed a compressive strength as high as 7.06 MPa, quite close to that of natural bone.					
31220495	3	60	theme	bi-templates	554:565	arg1	surface					535:541	the surface	531:541	the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm	531:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	7	61	theme	mouse	1031:1035	arg1	cells					1059:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	wild-type mouse embryonic fibroblasts cells	1021:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	1	62	theme	bone	157:160	arg1	formation					162:170	bone formation	157:170	bone formation	157:170	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	3	63	from	300 μm	647:652	arg1	size					627:630	pore size	622:630	pore size from 100 μm to 300 μm	622:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	8	64	from	resistance	1195:1204	arg1	good					1175:1178	good	1175:1178	good	1175:1178	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	8	65	from	ratio	1288:1292	arg1	weight					1269:1274	initial weight left	1261:1279	initial weight left at the ratio of Col to CMC of 1:9	1261:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	8	65	from	ratio	1288:1292	arg1	%					1256:1256	about 50%	1248:1256	about 50% of initial weight left at the ratio of Col to CMC of 1:9	1248:1313	The organic-inorganic composite also behaved good in collagenase resistance and could be biodegraded in 8 weeks, with about 50% of initial weight left at the ratio of Col to CMC of 1:9.					
31220495	1	66	theme	formation	162:170	arg1	mechanism					144:152	the mechanism	140:152	the mechanism of bone formation	140:170	Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules.					
31220495	4	67	theme	-85	716:718	arg1	%					715:715	%	715:715	%	715:715	The porosities of composites were located at the range of 71%-85%.					
31220495	5	68	theme	Col	806:808	arg1	ratio					797:801	the ratio	793:801	the ratio of Col to CMC in the organic template	793:839	Besides, the compressive strength of composites was highly depended on the ratio of Col to CMC in the organic template.					
31220495	7	69	theme	relative	997:1004	arg1	rate					1013:1016	The high relative growth rate	988:1016	The high relative growth rate of wild-type mouse embryonic fibroblasts cells	988:1063	The high relative growth rate of wild-type mouse embryonic fibroblasts cells was found for the composite, indicating a good biocompatibility.					
31220495	2	70	theme	composites	427:436	arg1	composition					377:387	composition	377:387	composition	377:387	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	2	70	theme	composites	427:436	arg1	properties					402:411	physical properties	393:411	physical properties	393:411	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	2	70	theme	composites	427:436	arg1	morphology					365:374	morphology	365:374	morphology	365:374	The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically.					
31220495	3	71	theme	composite	577:585	arg1	structure					607:615	the composite presented 3D porous structure	573:615	the composite presented 3D porous structure with pore size from 100 μm to 300 μm	573:652	It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm.					
31220495	9	72	theme	rational	1409:1416	arg1	method					1427:1432	a rational and safe method	1407:1432	a rational and safe method to prepare biomaterials with tunable properties	1407:1480	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31220495	9	72	theme	rational	1409:1416	arg1	composite					1361:1369	the Col-CMC/HA composite	1346:1369	the Col-CMC/HA composite by bi-molecular template method	1346:1401	The results demonstrated that the Col-CMC/HA composite by bi-molecular template method was a rational and safe method to prepare biomaterials with tunable properties.					
31545583	0	0	theme	Oligosaccharide	81:95	arg1	Profiles					116:123	the Oligosaccharide and Polysaccharide Profiles	77:123	Profiles	116:123	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	4	1	with	ones	896:899	arg1	syrup					911:915	corn syrup	906:915	corn syrup	906:915	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	1	with	ones	896:899	arg1	syrup					927:931	invert syrup	920:931	invert syrup	920:931	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	5	2	theme	high-carbohydrate	1220:1236	arg1	foods					1238:1242	other high-carbohydrate foods	1214:1242	other high-carbohydrate foods	1214:1242	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	4	3	theme	tetrasaccharides	993:1008	arg1	[M+Cl					947:951	the [M+Cl]-	943:953	the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides	943:1008	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	3	theme	tetrasaccharides	993:1008	arg1	fragmented					1015:1024	fragmented	1015:1024	fragmented	1015:1024	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	3	4	theme	high-fructose	706:718	arg1	syrup					725:729	high-fructose corn syrup	706:729	high-fructose corn syrup	706:729	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	5	theme	corn	906:909	arg1	syrup					911:915	corn syrup	906:915	corn syrup	906:915	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	3	6	used	used	637:640	arg2	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	6	used	used	637:640	arg2	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	5	7	theme	foods	1238:1242	arg1	identification					1196:1209	the identification	1192:1209	the identification of other high-carbohydrate foods	1192:1242	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	0	8	theme	Polysaccharide	101:114	arg1	Profiles					116:123	the Oligosaccharide and Polysaccharide Profiles	77:123	Profiles	116:123	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	3	9	theme	corn	720:723	arg1	syrup					725:729	high-fructose corn syrup	706:729	high-fructose corn syrup	706:729	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	10	theme	invert	920:925	arg1	syrup					927:931	invert syrup	920:931	invert syrup	920:931	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	11	from	difference	801:810	arg1	composition					819:829	the composition	815:829	the composition of oligosaccharide isomers	815:856	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	2	12	theme	mass	312:315	arg1	MALDI-MS					331:338	MALDI-MS	331:338	MALDI-MS	331:338	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	12	theme	mass	312:315	arg1	spectrometry					317:328	matrix-assisted laser desorption/ionization mass spectrometry	268:328	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	268:339	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	3	13	theme	saccharides	580:590	arg1	trends					570:575	abnormal trends	561:575	abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	561:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	13	theme	saccharides	580:590	arg1	polymerization					532:545	polymerization	532:545	polymerization (DP ≥ 13)	532:555	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	13	theme	saccharides	580:590	arg1	DP					548:549	DP ≥ 13	548:554	DP ≥ 13	548:554	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	14	theme	trends	570:575	arg1	degree					522:527	higher degree	515:527	higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	515:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	15	with	samples	872:878	arg1	syrup					911:915	corn syrup	906:915	corn syrup	906:915	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	15	with	samples	872:878	arg1	syrup					927:931	invert syrup	920:931	invert syrup	920:931	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	5	16	theme	high-throughput	1107:1121	arg1	monitoring					1123:1132	the high-throughput monitoring	1103:1132	the high-throughput monitoring of honey adulteration	1103:1154	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	2	17	theme	desorption/ionization	290:310	arg1	MALDI-MS					331:338	MALDI-MS	331:338	MALDI-MS	331:338	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	17	theme	desorption/ionization	290:310	arg1	spectrometry					317:328	matrix-assisted laser desorption/ionization mass spectrometry	268:328	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	268:339	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	0	18	theme	High-Throughput	0:14	arg1	Monitoring					16:25	High-Throughput Monitoring	0:25	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey	0:66	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	4	19	theme	product	1045:1051	arg1	pairs					1057:1061	diagnostic product ion pairs	1034:1061	diagnostic product ion pairs	1034:1061	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	20	from	ones	896:899	arg1	[M+Cl					947:951	the [M+Cl]-	943:953	the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides	943:1008	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	20	from	ones	896:899	arg1	fragmented					1015:1024	fragmented	1015:1024	fragmented	1015:1024	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	21	theme	adulterated	884:894	arg1	ones					896:899	adulterated ones	884:899	adulterated ones	884:899	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	2	22	theme	laser	284:288	arg1	MALDI-MS					331:338	MALDI-MS	331:338	MALDI-MS	331:338	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	22	theme	laser	284:288	arg1	spectrometry					317:328	matrix-assisted laser desorption/ionization mass spectrometry	268:328	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	268:339	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	3	23	theme	corn	735:738	arg1	syrup					740:744	corn syrup	735:744	corn syrup	735:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	2	24	theme	matrix-assisted	268:282	arg1	MALDI-MS					331:338	MALDI-MS	331:338	MALDI-MS	331:338	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	24	theme	matrix-assisted	268:282	arg1	spectrometry					317:328	matrix-assisted laser desorption/ionization mass spectrometry	268:328	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	268:339	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	3	25	with	adulteration	688:699	arg1	syrup					725:729	high-fructose corn syrup	706:729	high-fructose corn syrup	706:729	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	25	with	adulteration	688:699	arg1	syrup					740:744	corn syrup	735:744	corn syrup	735:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	2	26	theme	oligosaccharide	402:416	arg1	profiles					437:444	oligosaccharide and polysaccharide profiles	402:444	profiles	437:444	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	4	27	theme	ion	1053:1055	arg1	pairs					1057:1061	diagnostic product ion pairs	1034:1061	diagnostic product ion pairs	1034:1061	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	5	28	theme	honey	1137:1141	arg1	adulteration					1143:1154	honey adulteration	1137:1154	honey adulteration	1137:1154	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	3	29	from	degree	522:527	arg1	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	29	from	degree	522:527	arg1	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	0	30	from	Monitoring	16:25	arg1	Honey					62:66	Honey	62:66	Honey	62:66	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	3	31	theme	honey	682:686	arg1	adulteration					688:699	honey adulteration	682:699	honey adulteration with high-fructose corn syrup and corn syrup	682:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	32	theme	adulterated	595:605	arg1	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	32	theme	adulterated	595:605	arg1	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	33	theme	oligosaccharide	834:848	arg1	isomers					850:856	oligosaccharide isomers	834:856	oligosaccharide isomers	834:856	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	0	34	theme	Mass	134:137	arg1	Spectrometry					139:150	MALDI Mass Spectrometry	128:150	MALDI Mass Spectrometry	128:150	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	3	35	from	polymerization	532:545	arg1	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	35	from	polymerization	532:545	arg1	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	36	from	samples	613:619	arg1	degree					522:527	higher degree	515:527	higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	515:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	37	theme	polysaccharides	494:508	arg1	presence					482:489	the presence	478:489	the presence of polysaccharides	478:508	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	0	38	theme	MALDI	128:132	arg1	Spectrometry					139:150	MALDI Mass Spectrometry	128:150	MALDI Mass Spectrometry	128:150	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	1	39	theme	natural	164:170	arg1	product					172:178	a natural product	162:178	a natural product that could be easily adulterated with various cheaper sweeteners	162:243	Honey is a natural product that could be easily adulterated with various cheaper sweeteners.					
31545583	1	39	theme	natural	164:170	arg1	Honey					153:157	Honey	153:157	Honey	153:157	Honey is a natural product that could be easily adulterated with various cheaper sweeteners.					
31545583	3	40	theme	≥	551:551	arg1	DP					548:549	DP ≥ 13	548:554	DP ≥ 13	548:554	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	40	theme	≥	551:551	arg1	polymerization					532:545	polymerization	532:545	polymerization (DP ≥ 13)	532:555	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	0	41	theme	Adulterants	47:57	arg1	Monitoring					16:25	High-Throughput Monitoring	0:25	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey	0:66	High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.					
31545583	5	42	theme	other	1214:1218	arg1	foods					1238:1242	other high-carbohydrate foods	1214:1242	other high-carbohydrate foods	1214:1242	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	3	43	theme	MS-based	447:454	arg1	strategy					456:463	MS-based strategy	447:463	MS-based strategy	447:463	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	44	theme	isomers	850:856	arg1	composition					819:829	the composition	815:829	the composition of oligosaccharide isomers	815:856	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	45	from	samples	872:878	arg1	[M+Cl					947:951	the [M+Cl]-	943:953	the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides	943:1008	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	45	from	samples	872:878	arg1	fragmented					1015:1024	fragmented	1015:1024	fragmented	1015:1024	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	1	46	theme	various	218:224	arg1	sweeteners					234:243	various cheaper sweeteners	218:243	various cheaper sweeteners	218:243	Honey is a natural product that could be easily adulterated with various cheaper sweeteners.					
31545583	3	47	from	trends	570:575	arg1	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	47	from	trends	570:575	arg1	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	48	theme	disaccharides	958:970	arg1	[M+Cl					947:951	the [M+Cl]-	943:953	the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides	943:1008	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	48	theme	disaccharides	958:970	arg1	fragmented					1015:1024	fragmented	1015:1024	fragmented	1015:1024	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	3	49	theme	honey	607:611	arg1	indicators					645:654	indicators	645:654	indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	645:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	49	theme	honey	607:611	arg1	samples					613:619	adulterated honey samples	595:619	adulterated honey samples	595:619	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	3	50	theme	adulteration	688:699	arg1	identification					664:677	the identification	660:677	the identification of honey adulteration with high-fructose corn syrup and corn syrup	660:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	51	theme	honey	866:870	arg1	samples					872:878	honey samples	866:878	honey samples	866:878	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	3	52	theme	polymerization	532:545	arg1	degree					522:527	higher degree	515:527	higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	515:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	53	theme	trisaccharides	973:986	arg1	[M+Cl					947:951	the [M+Cl]-	943:953	the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides	943:1008	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	53	theme	trisaccharides	973:986	arg1	fragmented					1015:1024	fragmented	1015:1024	fragmented	1015:1024	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	54	from	fragmented	1015:1024	arg1	ones					896:899	adulterated ones	884:899	adulterated ones	884:899	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	4	54	from	fragmented	1015:1024	arg1	samples					872:878	honey samples	866:878	honey samples	866:878	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	3	55	theme	higher	515:520	arg1	degree					522:527	higher degree	515:527	higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	515:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	1	56	theme	cheaper	226:232	arg1	sweeteners					234:243	various cheaper sweeteners	218:243	various cheaper sweeteners	218:243	Honey is a natural product that could be easily adulterated with various cheaper sweeteners.					
31545583	3	57	theme	abnormal	561:568	arg1	trends					570:575	abnormal trends	561:575	abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup	561:744	MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup.					
31545583	4	58	theme	MS/MS-based	747:757	arg1	strategy					759:766	MS/MS-based strategy	747:766	MS/MS-based strategy	747:766	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	2	59	theme	polysaccharide	422:435	arg1	profiles					437:444	oligosaccharide and polysaccharide profiles	402:444	profiles	437:444	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	60	theme	present	253:259	arg1	study					261:265	the present study	249:265	the present study	249:265	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	2	61	theme	adulteration	380:391	arg1	detection					361:369	the detection	357:369	the detection of honey adulteration based on oligosaccharide and polysaccharide profiles	357:444	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	4	62	theme	diagnostic	1034:1043	arg1	pairs					1057:1061	diagnostic product ion pairs	1034:1061	diagnostic product ion pairs	1034:1061	MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs.					
31545583	5	63	theme	adulteration	1143:1154	arg1	monitoring					1123:1132	the high-throughput monitoring	1103:1132	the high-throughput monitoring of honey adulteration	1103:1154	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31545583	2	64	theme	honey	374:378	arg1	adulteration					380:391	honey adulteration	374:391	honey adulteration	374:391	In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.					
31545583	5	65	theme	new	1172:1174	arg1	perspective					1176:1186	a new perspective	1170:1186	a new perspective for the identification of other high-carbohydrate foods	1170:1242	The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.					
31826450	8	0	theme	stable	1216:1221	arg1	foams					1223:1227	stable foams	1216:1227	stable foams	1216:1227	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	3	1	contain	contained	354:362	arg2	homogalacturonan					400:415	a relatively high methyl-esterified homogalacturonan	364:415	a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains	364:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	3	1	contain	contained	354:362	arg1	fraction					345:352	The fraction	341:352	The fraction	341:352	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	0	2	theme	fraction	90:97	arg1	properties					39:48	Structural, rheological and functional properties	0:48	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek	0:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	6	3	theme	length	970:975	arg1	chains					952:957	β-(1→4)-linked galactan chains	928:957	β-(1→4)-linked galactan chains of varying length	928:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	3	4	theme	methyl-esterified	382:398	arg1	homogalacturonan					400:415	a relatively high methyl-esterified homogalacturonan	364:415	a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains	364:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	6	5	theme	varying	962:968	arg1	length					970:975	varying length	962:975	varying length	962:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	7	6	theme	thixotropic	1032:1042	arg1	behavior					1081:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior	1007:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide	1007:1110	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	7	7	theme	shear-thinning	1011:1024	arg1	behavior					1081:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior	1007:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide	1007:1110	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	1	8	theme	acid-extracted	113:126	arg1	polysaccharide					128:141	An acid-extracted polysaccharide	110:141	An acid-extracted polysaccharide from alchohol-insoluble solids of leek	110:180	An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained.					
31826450	8	9	theme	high	1232:1235	arg1	concentration					1237:1249	high concentration	1232:1249	high concentration	1232:1249	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	4	10	theme	Mw	531:532	arg1	populations					534:544	three high Mw populations	520:544	three high Mw populations	520:544	The fraction consisted of three high Mw populations, covering the range of 10-100 kDa.					
31826450	6	11	theme	unbranched	870:879	arg1	segments					881:888	unbranched segments	870:888	unbranched segments	870:888	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	4	12	theme	high	526:529	arg1	populations					534:544	three high Mw populations	520:544	three high Mw populations	520:544	The fraction consisted of three high Mw populations, covering the range of 10-100 kDa.					
31826450	8	13	theme	higher	1134:1139	arg1	capacity					1155:1162	higher water holding capacity	1134:1162	higher water holding capacity	1134:1162	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	2	14	theme	small	300:304	arg1	amounts					306:312	small amounts	300:312	small amounts of rhamnose and arabinose	300:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	14	theme	small	300:304	arg1	arabinose					330:338	arabinose	330:338	arabinose	330:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	14	theme	small	300:304	arg1	rhamnose					317:324	rhamnose	317:324	rhamnose	317:324	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	3	15	theme	high	377:380	arg1	homogalacturonan					400:415	a relatively high methyl-esterified homogalacturonan	364:415	a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains	364:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	0	16	from	properties	39:48	arg1	leek					104:107	leek	104:107	leek	104:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	5	17	theme	degrading	633:641	arg1	enzymes					643:649	HG/RG-I degrading enzymes	625:649	HG/RG-I degrading enzymes	625:649	Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure.					
31826450	3	18	theme	rhamnogalacturonan	425:442	arg1	type					444:447	rhamnogalacturonan type I	425:449	rhamnogalacturonan type I decorated with galactose-rich side chains	425:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	6	19	theme	-linked	935:941	arg1	chains					952:957	β-(1→4)-linked galactan chains	928:957	β-(1→4)-linked galactan chains of varying length	928:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	8	20	theme	holding	1147:1153	arg1	capacity					1155:1162	higher water holding capacity	1134:1162	higher water holding capacity	1134:1162	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	1	21	from	solids	167:172	arg1	polysaccharide					128:141	An acid-extracted polysaccharide	110:141	An acid-extracted polysaccharide from alchohol-insoluble solids of leek	110:180	An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained.					
31826450	3	22	theme	side	481:484	arg1	chains					486:491	galactose-rich side chains	466:491	galactose-rich side chains	466:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	0	23	theme	Structural	0:9	arg1	properties					39:48	Structural, rheological and functional properties	0:48	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek	0:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	8	24	theme	water	1141:1145	arg1	capacity					1155:1162	higher water holding capacity	1134:1162	higher water holding capacity	1134:1162	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	7	25	theme	rheological	982:992	arg1	study					994:998	The rheological study	978:998	The rheological study	978:998	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	2	26	theme	arabinose	330:338	arg1	amounts					306:312	small amounts	300:312	small amounts of rhamnose and arabinose	300:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	26	theme	arabinose	330:338	arg1	arabinose					330:338	arabinose	330:338	arabinose	330:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	26	theme	arabinose	330:338	arg1	rhamnose					317:324	rhamnose	317:324	rhamnose	317:324	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	6	27	theme	methyl-esterification	830:850	arg1	RG-I					853:856	RG-I	853:856	RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length	853:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	6	27	theme	methyl-esterification	830:850	arg1	patterns					818:825	different patterns	808:825	different patterns of methyl-esterification	808:850	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	0	28	theme	rheological	12:22	arg1	properties					39:48	Structural, rheological and functional properties	0:48	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek	0:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	7	29	theme	non-Newtonian	1067:1079	arg1	behavior					1081:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior	1007:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide	1007:1110	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	2	30	theme	rhamnose	317:324	arg1	amounts					306:312	small amounts	300:312	small amounts of rhamnose and arabinose	300:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	30	theme	rhamnose	317:324	arg1	arabinose					330:338	arabinose	330:338	arabinose	330:338	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	30	theme	rhamnose	317:324	arg1	rhamnose					317:324	rhamnose	317:324	rhamnose	317:324	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	6	31	link	-linked	935:941	arg1	chains					952:957	β-(1→4)-linked galactan chains	928:957	β-(1→4)-linked galactan chains of varying length	928:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	2	32	theme	sugar	200:204	arg1	composition					206:216	The sugar composition	196:216	The sugar composition	196:216	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	0	33	theme	functional	28:37	arg1	properties					39:48	Structural, rheological and functional properties	0:48	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek	0:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	3	34	theme	galactose-rich	466:479	arg1	chains					486:491	galactose-rich side chains	466:491	galactose-rich side chains	466:491	The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains.					
31826450	0	35	theme	galactose-rich	53:66	arg1	fraction					90:97	galactose-rich pectic polysaccharide fraction	53:97	galactose-rich pectic polysaccharide fraction from leek	53:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	4	36	theme	10-100 kDa	569:578	arg1	range					560:564	the range	556:564	the range of 10-100 kDa	556:578	The fraction consisted of three high Mw populations, covering the range of 10-100 kDa.					
31826450	6	37	theme	galactan	943:950	arg1	chains					952:957	β-(1→4)-linked galactan chains	928:957	β-(1→4)-linked galactan chains of varying length	928:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	8	38	theme	oil-holding	1169:1179	arg1	capacity					1181:1188	oil-holding capacity	1169:1188	oil-holding capacity	1169:1188	The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.					
31826450	7	39	theme	anti-thixotropic	1045:1060	arg1	behavior					1081:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior	1007:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide	1007:1110	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	2	40	theme	galacturonic	247:258	arg1	sugars					280:285	the major sugars	270:285	the major sugars	270:285	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	40	theme	galacturonic	247:258	arg1	galactose					233:241	galactose	233:241	galactose	233:241	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	40	theme	galacturonic	247:258	arg1	acid					260:263	galacturonic acid	247:263	galacturonic acid	247:263	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	6	41	theme	different	808:816	arg1	RG-I					853:856	RG-I	853:856	RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length	853:975	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	6	41	theme	different	808:816	arg1	patterns					818:825	different patterns	808:825	different patterns of methyl-esterification	808:850	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	7	42	dep	shear-thinning	1011:1024	arg1	weak					1027:1030	weak	1027:1030	weak	1027:1030	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	7	43	theme	polysaccharide	1097:1110	arg1	behavior					1081:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior	1007:1088	the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide	1007:1110	The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide.					
31826450	5	44	theme	HG/RG-I	625:631	arg1	enzymes					643:649	HG/RG-I degrading enzymes	625:649	HG/RG-I degrading enzymes	625:649	Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure.					
31826450	1	45	theme	alchohol-insoluble	148:165	arg1	solids					167:172	alchohol-insoluble solids	148:172	alchohol-insoluble solids of leek	148:180	An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained.					
31826450	6	46	theme	GalA	785:788	arg1	sequences					790:798	GalA sequences	785:798	GalA sequences	785:798	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	0	47	theme	polysaccharide	75:88	arg1	fraction					90:97	galactose-rich pectic polysaccharide fraction	53:97	galactose-rich pectic polysaccharide fraction from leek	53:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	0	48	from	leek	104:107	arg1	fraction					90:97	galactose-rich pectic polysaccharide fraction	53:97	galactose-rich pectic polysaccharide fraction from leek	53:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	0	48	from	leek	104:107	arg1	properties					39:48	Structural, rheological and functional properties	0:48	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek	0:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	6	49	theme	sequences	790:798	arg1	presence					773:780	the presence	769:780	the presence of GalA sequences	769:798	The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length.					
31826450	0	50	theme	pectic	68:73	arg1	fraction					90:97	galactose-rich pectic polysaccharide fraction	53:97	galactose-rich pectic polysaccharide fraction from leek	53:107	Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek.					
31826450	2	51	theme	major	274:278	arg1	sugars					280:285	the major sugars	270:285	the major sugars	270:285	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	51	theme	major	274:278	arg1	galactose					233:241	galactose	233:241	galactose	233:241	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	2	51	theme	major	274:278	arg1	acid					260:263	galacturonic acid	247:263	galacturonic acid	247:263	The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose.					
31826450	5	52	theme	Enzymatic	581:589	arg1	fingerprinting					591:604	Enzymatic fingerprinting	581:604	Enzymatic fingerprinting	581:604	Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure.					
31826450	1	53	theme	leek	177:180	arg1	solids					167:172	alchohol-insoluble solids	148:172	alchohol-insoluble solids of leek	148:180	An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained.					
31551153	10	0	theme	higher	1126:1131	arg1	properties					1141:1150	higher tensile properties	1126:1150	higher tensile properties of the fibers	1126:1164	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	1	1	theme	dental	279:284	arg1	composites					286:295	experimental dental composites	266:295	experimental dental composites	266:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	11	2	theme	perpendicular	1242:1254	arg1	orientation					1256:1266	perpendicular orientation	1242:1266	perpendicular orientation	1242:1266	Fibers presented anisotropic behavior with higher UTS and E when tested in perpendicular orientation.					
31551153	5	3	theme	collector	633:641	arg1	drum					643:646	the collector drum	629:646	the collector drum	629:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	5	4	theme	parallel	571:578	arg1	orientation					559:569	an orientation	556:569	an orientation parallel and perpendicular to the rotational direction of the collector drum	556:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	1	5	theme	nanocrystal	186:196	arg1	nanofibers					229:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	10	6	theme	RESULTS	1087:1093	arg1	Addition					1095:1102	RESULTS Addition	1087:1102	RESULTS Addition of 3% NCC	1087:1112	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	1	7	theme	composites	286:295	arg1	properties					252:261	flexural properties	243:261	flexural properties of experimental dental composites	243:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	5	8	theme	drum	643:646	arg1	direction					616:624	the rotational direction	601:624	the rotational direction of the collector drum	601:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	2	9	theme	dimethylformamide	319:335	arg1	solution					343:350	dimethylformamide (DMF) solution	319:350	dimethylformamide (DMF) solution	319:350	METHODS 11wt% PAN in dimethylformamide (DMF) solution was electrospun at 17.2kVA and 20cm from the collector drum.					
31551153	14	10	theme	electrospun	1627:1637	arg1	nanofibres					1639:1648	electrospun nanofibres	1627:1648	electrospun nanofibres	1627:1648	This fundamental study warrants future investigation in the use of electrospun nanofibres as a way to reinforce dental composites.					
31551153	12	11	theme	NCC-PAN	1293:1299	arg1	nanofibers					1301:1310	3% NCC-PAN nanofibers	1290:1310	3% NCC-PAN nanofibers	1290:1310	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	5	12	theme	perpendicular	584:596	arg1	orientation					559:569	an orientation	556:569	an orientation parallel and perpendicular to the rotational direction of the collector drum	556:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	10	13	theme	fibers	1159:1164	arg1	properties					1141:1150	higher tensile properties	1126:1150	higher tensile properties of the fibers	1126:1164	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	12	14	theme	beams	1420:1424	arg1	fracture					1356:1363	fracture	1356:1363	fracture	1356:1363	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	12	14	theme	beams	1420:1424	arg1	strength					1378:1385	flexural strength	1369:1385	flexural strength of experimental dental composite beams	1369:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	14	15	theme	nanofibres	1639:1648	arg1	use					1620:1622	the use	1616:1622	the use of electrospun nanofibres as a way to reinforce dental composites	1616:1688	This fundamental study warrants future investigation in the use of electrospun nanofibres as a way to reinforce dental composites.					
31551153	12	16	theme	%	1291:1291	arg1	nanofibers					1301:1310	3% NCC-PAN nanofibers	1290:1310	3% NCC-PAN nanofibers	1290:1310	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	6	17	theme	maximum	753:759	arg1	stress					761:766	maximum stress	753:766	maximum stress	753:766	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	2	18	theme	%	310:310	arg1	PAN					312:314	% PAN	310:314	% PAN in dimethylformamide (DMF) solution	310:350	METHODS 11wt% PAN in dimethylformamide (DMF) solution was electrospun at 17.2kVA and 20cm from the collector drum.					
31551153	1	19	theme	NCC	209:211	arg1	nanofibers					229:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	1	20	theme	cellulose	198:206	arg1	nanofibers					229:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	5	21	theme	rotational	605:614	arg1	direction					616:624	the rotational direction	601:624	the rotational direction of the collector drum	601:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	6	22	theme	stress/strain	793:805	arg1	plots					807:811	stress/strain plots	793:811	stress/strain plots	793:811	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	13	23	theme	experimental	1497:1508	arg1	composites					1517:1526	experimental dental composites	1497:1526	experimental dental composites	1497:1526	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	6	24	theme	ultimate	682:689	arg1	UTS					709:711	UTS	709:711	UTS	709:711	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	24	theme	ultimate	682:689	arg1	strength					699:706	ultimate tensile strength	682:706	ultimate tensile strength (UTS)	682:712	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	11	25	theme	higher	1210:1215	arg1	UTS					1217:1219	higher UTS	1210:1219	higher UTS	1210:1219	Fibers presented anisotropic behavior with higher UTS and E when tested in perpendicular orientation.					
31551153	12	26	theme	nanofibers	1301:1310	arg1	incorporation					1273:1285	The incorporation	1269:1285	The incorporation of 3% NCC-PAN nanofibers	1269:1310	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	2	27	theme	collector	397:405	arg1	drum					407:410	the collector drum	393:410	the collector drum	393:410	METHODS 11wt% PAN in dimethylformamide (DMF) solution was electrospun at 17.2kVA and 20cm from the collector drum.					
31551153	0	28	theme	Experimental	0:11	arg1	composites					13:22	Experimental composites	0:22	Experimental composites of polyacrilonitrile-electrospun nanofibers	0:66	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	0	28	theme	Experimental	0:11	arg1	nanofibers					57:66	polyacrilonitrile-electrospun nanofibers	27:66	polyacrilonitrile-electrospun nanofibers	27:66	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	13	29	theme	suitable	1462:1469	arg1	NCC					1440:1442	SIGNIFICANCE NCC	1427:1442	SIGNIFICANCE NCC	1427:1442	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	13	29	theme	suitable	1462:1469	arg1	nanoparticle					1471:1482	a suitable nanoparticle	1460:1482	a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber	1460:1557	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	14	30	theme	fundamental	1565:1575	arg1	study					1577:1581	This fundamental study	1560:1581	This fundamental study	1560:1581	This fundamental study warrants future investigation in the use of electrospun nanofibres as a way to reinforce dental composites.					
31551153	7	31	theme	50/50	866:870	arg1	%					888:888	50/50 BisGMA/TEGDMA wt%	866:888	50/50 BisGMA/TEGDMA wt%	866:888	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	7	31	theme	50/50	866:870	arg1	monomers					856:863	resin monomers	850:863	resin monomers (50/50 BisGMA/TEGDMA wt%)	850:889	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	6	32	theme	Tensile	649:655	arg1	tests					657:661	Tensile tests	649:661	Tensile tests	649:661	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	0	33	theme	polyacrilonitrile-electrospun	27:55	arg1	nanofibers					57:66	polyacrilonitrile-electrospun nanofibers	27:66	polyacrilonitrile-electrospun nanofibers	27:66	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	7	34	theme	BisGMA/TEGDMA	872:884	arg1	%					888:888	50/50 BisGMA/TEGDMA wt%	866:888	50/50 BisGMA/TEGDMA wt%	866:888	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	7	34	theme	BisGMA/TEGDMA	872:884	arg1	monomers					856:863	resin monomers	850:863	resin monomers (50/50 BisGMA/TEGDMA wt%)	850:889	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	9	35	theme	one-way	1063:1069	arg1	α=0.05					1078:1083	α=0.05	1078:1083	α=0.05	1078:1083	Data were analyzed by multiple t-test and one-way ANOVA (α=0.05).					
31551153	9	35	theme	one-way	1063:1069	arg1	ANOVA					1071:1075	one-way ANOVA	1063:1075	one-way ANOVA (α=0.05)	1063:1084	Data were analyzed by multiple t-test and one-way ANOVA (α=0.05).					
31551153	1	36	theme	-containing	213:223	arg1	nanofibers					229:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	12	37	theme	dental	1403:1408	arg1	beams					1420:1424	experimental dental composite beams	1390:1424	experimental dental composite beams	1390:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	12	38	theme	3	1290:1290	arg1	%					1291:1291	%	1291:1291	%	1291:1291	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	12	39	theme	strength	1378:1385	arg1	work					1348:1351	work	1348:1351	work of fracture and flexural strength of experimental dental composite beams	1348:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	1	40	theme	addition	135:142	arg1	effects					124:130	the effects	120:130	the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites	120:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	1	41	theme	PAN	225:227	arg1	nanofibers					229:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	nanocrystal cellulose (NCC)-containing PAN nanofibers	186:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	12	42	theme	composite	1410:1418	arg1	beams					1420:1424	experimental dental composite beams	1390:1424	experimental dental composite beams	1390:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	6	43	theme	tensile	691:697	arg1	UTS					709:711	UTS	709:711	UTS	709:711	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	43	theme	tensile	691:697	arg1	strength					699:706	ultimate tensile strength	682:706	ultimate tensile strength (UTS)	682:712	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	12	44	theme	flexural	1369:1376	arg1	strength					1378:1385	flexural strength	1369:1385	flexural strength of experimental dental composite beams	1369:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	13	45	theme	SIGNIFICANCE	1427:1438	arg1	NCC					1440:1442	SIGNIFICANCE NCC	1427:1442	SIGNIFICANCE NCC	1427:1442	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	13	45	theme	SIGNIFICANCE	1427:1438	arg1	nanoparticle					1471:1482	a suitable nanoparticle	1460:1482	a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber	1460:1557	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	2	46	from	PAN	312:314	arg1	solution					343:350	dimethylformamide (DMF) solution	319:350	dimethylformamide (DMF) solution	319:350	METHODS 11wt% PAN in dimethylformamide (DMF) solution was electrospun at 17.2kVA and 20cm from the collector drum.					
31551153	1	47	theme	nanofibers	229:238	arg1	addition					135:142	addition	135:142	addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers	135:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	0	48	theme	nanofibers	57:66	arg1	composites					13:22	Experimental composites	0:22	Experimental composites of polyacrilonitrile-electrospun nanofibers	0:66	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	0	48	theme	nanofibers	57:66	arg1	nanofibers					57:66	polyacrilonitrile-electrospun nanofibers	27:66	polyacrilonitrile-electrospun nanofibers	27:66	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	7	49	theme	resin	850:854	arg1	%					888:888	50/50 BisGMA/TEGDMA wt%	866:888	50/50 BisGMA/TEGDMA wt%	866:888	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	7	49	theme	resin	850:854	arg1	monomers					856:863	resin monomers	850:863	resin monomers (50/50 BisGMA/TEGDMA wt%)	850:889	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	12	50	theme	experimental	1390:1401	arg1	beams					1420:1424	experimental dental composite beams	1390:1424	experimental dental composite beams	1390:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	8	51	theme	universal	994:1002	arg1	machine					1012:1018	a universal testing machine	992:1018	a universal testing machine	992:1018	Beams (2×2×25mm) were cut from the slabs and tested in a universal testing machine.					
31551153	4	52	theme	Fiber	452:456	arg1	mats					458:461	Fiber mats	452:461	Fiber mats	452:461	Fiber mats were produced in triplicates and tested as-spun.					
31551153	0	53	theme	nanocrystal	79:89	arg1	cellulose					91:99	nanocrystal cellulose	79:99	nanocrystal cellulose	79:99	Experimental composites of polyacrilonitrile-electrospun nanofibers containing nanocrystal cellulose.					
31551153	12	54	from	increase	1336:1343	arg1	work					1348:1351	work	1348:1351	work of fracture and flexural strength of experimental dental composite beams	1348:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	14	55	theme	future	1592:1597	arg1	investigation					1599:1611	future investigation	1592:1611	future investigation	1592:1611	This fundamental study warrants future investigation in the use of electrospun nanofibres as a way to reinforce dental composites.					
31551153	6	56	from	stress	761:766	arg1	elongation					739:748	elongation	739:748	elongation at maximum stress (%)	739:770	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	56	from	stress	761:766	arg1	strength					699:706	ultimate tensile strength	682:706	ultimate tensile strength (UTS)	682:712	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	56	from	stress	761:766	arg1	modulus					723:729	elastic modulus	715:729	elastic modulus (E)	715:733	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	56	from	stress	761:766	arg1	E					732:732	E	732:732	E	732:732	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	56	from	stress	761:766	arg1	UTS					709:711	UTS	709:711	UTS	709:711	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	56	from	stress	761:766	arg1	%					769:769	%	769:769	%	769:769	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	1	57	theme	polyacrilonitrile	147:163	arg1	nanofibers					171:180	polyacrilonitrile (PAN) nanofibers	147:180	polyacrilonitrile (PAN) nanofibers	147:180	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	10	58	theme	NCC	1110:1112	arg1	Addition					1095:1102	RESULTS Addition	1087:1102	RESULTS Addition of 3% NCC	1087:1112	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	14	59	theme	dental	1672:1677	arg1	composites					1679:1688	dental composites	1672:1688	dental composites	1672:1688	This fundamental study warrants future investigation in the use of electrospun nanofibres as a way to reinforce dental composites.					
31551153	7	60	theme	wt	886:887	arg1	%					888:888	50/50 BisGMA/TEGDMA wt%	866:888	50/50 BisGMA/TEGDMA wt%	866:888	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	7	60	theme	wt	886:887	arg1	monomers					856:863	resin monomers	850:863	resin monomers (50/50 BisGMA/TEGDMA wt%)	850:889	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	6	61	theme	elastic	715:721	arg1	modulus					723:729	elastic modulus	715:729	elastic modulus (E)	715:733	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	6	61	theme	elastic	715:721	arg1	E					732:732	E	732:732	E	732:732	Tensile tests were performed and ultimate tensile strength (UTS), elastic modulus (E) and elongation at maximum stress (%) were calculated from stress/strain plots.					
31551153	5	62	from	mat	549:551	arg1	orientation					559:569	an orientation	556:569	an orientation parallel and perpendicular to the rotational direction of the collector drum	556:646	Strips (5cm×0.5cm) were cut from the mat in an orientation parallel and perpendicular to the rotational direction of the collector drum.					
31551153	10	63	theme	%	1108:1108	arg1	NCC					1110:1112	3% NCC	1107:1112	3% NCC	1107:1112	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	1	64	theme	flexural	243:250	arg1	properties					252:261	flexural properties	243:261	flexural properties of experimental dental composites	243:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	8	65	theme	testing	1004:1010	arg1	machine					1012:1018	a universal testing machine	992:1018	a universal testing machine	992:1018	Beams (2×2×25mm) were cut from the slabs and tested in a universal testing machine.					
31551153	13	66	theme	dental	1510:1515	arg1	composites					1517:1526	experimental dental composites	1497:1526	experimental dental composites	1497:1526	SIGNIFICANCE NCC was found to be a suitable nanoparticle to reinforce experimental dental composites by incorporation via nanofiber.					
31551153	7	67	theme	Fiber	814:818	arg1	mats					820:823	Fiber mats	814:823	Fiber mats	814:823	Fiber mats were then infiltrated by resin monomers (50/50 BisGMA/TEGDMA wt%), stacked in a mold (2×15×25) and light-cured.					
31551153	1	68	theme	PAN	166:168	arg1	nanofibers					171:180	polyacrilonitrile (PAN) nanofibers	147:180	polyacrilonitrile (PAN) nanofibers	147:180	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	10	69	theme	3	1107:1107	arg1	%					1108:1108	%	1108:1108	%	1108:1108	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	12	70	theme	fracture	1356:1363	arg1	work					1348:1351	work	1348:1351	work of fracture and flexural strength of experimental dental composite beams	1348:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	9	71	theme	multiple	1043:1050	arg1	t-test					1052:1057	multiple t-test	1043:1057	multiple t-test	1043:1057	Data were analyzed by multiple t-test and one-way ANOVA (α=0.05).					
31551153	1	72	from	effects	124:130	arg1	properties					252:261	flexural properties	243:261	flexural properties of experimental dental composites	243:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	11	73	theme	anisotropic	1184:1194	arg1	behavior					1196:1203	anisotropic behavior	1184:1203	anisotropic behavior	1184:1203	Fibers presented anisotropic behavior with higher UTS and E when tested in perpendicular orientation.					
31551153	12	74	theme	significant	1324:1334	arg1	increase					1336:1343	significant increase	1324:1343	significant increase in work of fracture and flexural strength of experimental dental composite beams	1324:1424	The incorporation of 3% NCC-PAN nanofibers resulted in significant increase in work of fracture and flexural strength of experimental dental composite beams.					
31551153	1	75	theme	nanofibers	171:180	arg1	addition					135:142	addition	135:142	addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers	135:238	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31551153	10	76	theme	tensile	1133:1139	arg1	properties					1141:1150	higher tensile properties	1126:1150	higher tensile properties of the fibers	1126:1164	RESULTS Addition of 3% NCC resulted in higher tensile properties of the fibers.					
31551153	1	77	theme	experimental	266:277	arg1	composites					286:295	experimental dental composites	266:295	experimental dental composites	266:295	OBJECTIVE To test the effects of addition of polyacrilonitrile (PAN) nanofibers and nanocrystal cellulose (NCC)-containing PAN nanofibers on flexural properties of experimental dental composites.					
31364687	0	0	theme	nano-sized	101:110	arg1	supplement					124:133	an oral nano-sized nutritional supplement	93:133	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	4	1	with	distribution	746:757	arg1	size					790:793	the average size	778:793	the average size of ∼10 nm	778:803	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	1	with	distribution	746:757	arg1	shape					819:823	spherical shape	809:823	spherical shape within the EPS matrix	809:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	1	2	dep	stabilizing	335:345	arg1	favorable					325:333	favorable	325:333	favorable	325:333	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	3	3	theme	EPS-stabilized	509:522	arg1	complex					545:551	a complex	543:551	a complex	543:551	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	3	3	theme	EPS-stabilized	509:522	arg1	NPs					536:538	EPS-stabilized biogenic Fe NPs	509:538	EPS-stabilized biogenic Fe NPs	509:538	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	1	4	theme	biocompatible	273:285	arg1	NPs					308:310	NPs	308:310	NPs	308:310	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	1	4	theme	biocompatible	273:285	arg1	nanoparticles					293:305	biocompatible metal nanoparticles	273:305	biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties	273:369	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	0	5	theme	oral	96:99	arg1	supplement					124:133	an oral nano-sized nutritional supplement	93:133	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	6	theme	composite	1106:1114	arg1	safety					1077:1082	very high safety	1067:1082	very high safety of the biosynthesized composite	1067:1114	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	0	7	dep	in	145:146	arg1	vivo					148:151	vivo	148:151	vivo	148:151	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	1	8	theme	metal	287:291	arg1	NPs					308:310	NPs	308:310	NPs	308:310	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	1	8	theme	metal	287:291	arg1	nanoparticles					293:305	biocompatible metal nanoparticles	273:305	biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties	273:369	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	6	9	theme	biosynthesized	1091:1104	arg1	composite					1106:1114	the biosynthesized composite	1087:1114	the biosynthesized composite	1087:1114	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	5	10	theme	deficiency	982:991	arg1	anemia					993:998	iron deficiency anemia	977:998	iron deficiency anemia	977:998	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	4	11	theme	average	782:788	arg1	size					790:793	the average size	778:793	the average size of ∼10 nm	778:803	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	3	12	theme	novel	563:567	arg1	reaction					582:589	a novel biosynthesis reaction	561:589	a novel biosynthesis reaction	561:589	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	1	13	theme	nanoparticles	293:305	arg1	production					259:268	the production	255:268	the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure	255:441	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	0	14	theme	nutritional	112:122	arg1	supplement					124:133	an oral nano-sized nutritional supplement	93:133	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	15	theme	friendly	1187:1194	arg1	method					1206:1211	an economical and environment friendly synthesis method	1157:1211	an economical and environment friendly synthesis method	1157:1211	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	0	16	theme	high	140:143	arg1	compatibility					153:165	high in vivo compatibility	140:165	high in vivo compatibility	140:165	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	5	17	theme	Fe	904:905	arg1	NPs					907:909	the EPS-stabilized Fe NPs	885:909	the EPS-stabilized Fe NPs	885:909	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	4	18	theme	Fe	766:767	arg1	NPs					769:771	the Fe NPs	762:771	the Fe NPs	762:771	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	6	19	theme	environment	1175:1185	arg1	method					1206:1211	an economical and environment friendly synthesis method	1157:1211	an economical and environment friendly synthesis method	1157:1211	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	5	20	theme	in	865:866	arg1	toxicity					873:880	the in vivo toxicity	861:880	the in vivo toxicity of the EPS-stabilized Fe NPs	861:909	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	1	21	theme	polyanionic	394:404	arg1	groups					417:422	polyanionic functional groups	394:422	polyanionic functional groups	394:422	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	4	22	theme	Physicochemical	592:606	arg1	characterization					608:623	Physicochemical characterization	592:623	Physicochemical characterization of the EPS-Fe complex	592:645	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	2	23	theme	Pantoea	465:471	arg1	sp					473:474	Pantoea sp	465:474	Pantoea sp	465:474	In the present work, Pantoea sp.					
31364687	4	24	theme	thermal	678:684	arg1	stability					686:694	high thermal stability	673:694	high thermal stability	673:694	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	24	theme	thermal	678:684	arg1	properties					716:725	desirable magnetic properties	697:725	desirable magnetic properties	697:725	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	0	25	theme	biogreen	18:25	arg1	synthesis					27:35	Bacteria-assisted biogreen synthesis	0:35	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	1	26	theme	functional	406:415	arg1	groups					417:422	polyanionic functional groups	394:422	polyanionic functional groups	394:422	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	2	27	dep	sp	473:474	arg1	work					459:462	the present work	447:462	the present work	447:462	In the present work, Pantoea sp.					
31364687	6	28	theme	fields	1246:1251	arg1	spectrum					1224:1231	a wide spectrum	1217:1231	a wide spectrum of potential fields in medicine	1217:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	3	29	theme	biosynthesis	569:580	arg1	reaction					582:589	a novel biosynthesis reaction	561:589	a novel biosynthesis reaction	561:589	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	6	30	theme	high	1072:1075	arg1	safety					1077:1082	very high safety	1067:1082	very high safety of the biosynthesized composite	1067:1114	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	4	31	theme	desirable	697:705	arg1	stability					686:694	high thermal stability	673:694	high thermal stability	673:694	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	31	theme	desirable	697:705	arg1	properties					716:725	desirable magnetic properties	697:725	desirable magnetic properties	697:725	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	0	32	theme	Bacteria-assisted	0:16	arg1	synthesis					27:35	Bacteria-assisted biogreen synthesis	0:35	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	33	from	fields	1246:1251	arg1	medicine					1256:1263	medicine	1256:1263	medicine	1256:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	0	34	with	supplement	124:133	arg1	compatibility					153:165	high in vivo compatibility	140:165	high in vivo compatibility	140:165	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	35	theme	economical	1160:1169	arg1	method					1206:1211	an economical and environment friendly synthesis method	1157:1211	an economical and environment friendly synthesis method	1157:1211	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	5	36	theme	EPS-stabilized	889:902	arg1	NPs					907:909	the EPS-stabilized Fe NPs	885:909	the EPS-stabilized Fe NPs	885:909	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	1	37	from	presence	382:389	arg1	structure					433:441	their structure	427:441	their structure	427:441	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	5	38	theme	anemia	993:998	arg1	treatment					964:972	the treatment	960:972	the treatment of iron deficiency anemia	960:998	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	6	39	theme	histology	1039:1047	arg1	studies					1049:1055	organ histology studies	1033:1055	organ histology studies	1033:1055	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	5	40	dep	in	865:866	arg1	vivo					868:871	vivo	868:871	vivo	868:871	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	2	41	theme	present	451:457	arg1	work					459:462	the present work	447:462	the present work	447:462	In the present work, Pantoea sp.					
31364687	6	42	from	medicine	1256:1263	arg1	spectrum					1224:1231	a wide spectrum	1217:1231	a wide spectrum of potential fields in medicine	1217:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	6	43	theme	synthesis	1196:1204	arg1	method					1206:1211	an economical and environment friendly synthesis method	1157:1211	an economical and environment friendly synthesis method	1157:1211	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	0	44	theme	in	145:146	arg1	compatibility					153:165	high in vivo compatibility	140:165	high in vivo compatibility	140:165	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	1	45	theme	groups	417:422	arg1	presence					382:389	the presence	378:389	the presence of polyanionic functional groups in their structure	378:441	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	3	46	theme	biogenic	524:531	arg1	complex					545:551	a complex	543:551	a complex	543:551	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	3	46	theme	biogenic	524:531	arg1	NPs					536:538	EPS-stabilized biogenic Fe NPs	509:538	EPS-stabilized biogenic Fe NPs	509:538	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	3	47	theme	Fe	533:534	arg1	complex					545:551	a complex	543:551	a complex	543:551	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	3	47	theme	Fe	533:534	arg1	NPs					536:538	EPS-stabilized biogenic Fe NPs	509:538	EPS-stabilized biogenic Fe NPs	509:538	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	0	48	theme	scavenging	48:57	arg1	complexes					82:90	radical scavenging exopolysaccharide-iron complexes	40:90	radical scavenging exopolysaccharide-iron complexes	40:90	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	4	49	theme	high	673:676	arg1	stability					686:694	high thermal stability	673:694	high thermal stability	673:694	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	49	theme	high	673:676	arg1	properties					716:725	desirable magnetic properties	697:725	desirable magnetic properties	697:725	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	3	50	used	used	493:496	arg2	GH					486:487	BCCS 001 GH	477:487	BCCS 001 GH	477:487	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	1	51	theme	efficient	231:239	arg1	substrate					241:249	an efficient substrate	228:249	an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure	228:441	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	0	52	theme	radical	40:46	arg1	complexes					82:90	radical scavenging exopolysaccharide-iron complexes	40:90	radical scavenging exopolysaccharide-iron complexes	40:90	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	53	theme	potential	1236:1244	arg1	fields					1246:1251	potential fields	1236:1251	potential fields in medicine	1236:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	3	54	theme	BCCS	477:480	arg1	GH					486:487	BCCS 001 GH	477:487	BCCS 001 GH	477:487	BCCS 001 GH was used to produce EPS-stabilized biogenic Fe NPs as a complex through a novel biosynthesis reaction.					
31364687	0	55	theme	complexes	82:90	arg1	synthesis					27:35	Bacteria-assisted biogreen synthesis	0:35	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	1	56	theme	stabilizing	335:345	arg1	properties					360:369	their favorable stabilizing and reducing properties	319:369	their favorable stabilizing and reducing properties	319:369	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	4	57	theme	EPS	836:838	arg1	matrix					840:845	the EPS matrix	832:845	the EPS matrix	832:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	58	theme	uniform	738:744	arg1	distribution					746:757	the uniform distribution	734:757	the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix	734:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	0	59	theme	exopolysaccharide-iron	59:80	arg1	complexes					82:90	radical scavenging exopolysaccharide-iron complexes	40:90	radical scavenging exopolysaccharide-iron complexes	40:90	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	6	60	with	candidate	1142:1150	arg1	method					1206:1211	an economical and environment friendly synthesis method	1157:1211	an economical and environment friendly synthesis method	1157:1211	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	5	61	theme	NPs	907:909	arg1	toxicity					873:880	the in vivo toxicity	861:880	the in vivo toxicity of the EPS-stabilized Fe NPs	861:909	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	1	62	theme	reducing	351:358	arg1	properties					360:369	their favorable stabilizing and reducing properties	319:369	their favorable stabilizing and reducing properties	319:369	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	5	63	theme	iron	977:980	arg1	anemia					993:998	iron deficiency anemia	977:998	iron deficiency anemia	977:998	In addition, the in vivo toxicity of the EPS-stabilized Fe NPs was evaluated to investigate their potential for the treatment of iron deficiency anemia.					
31364687	4	64	theme	nm	802:803	arg1	size					790:793	the average size	778:793	the average size of ∼10 nm	778:803	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	64	theme	nm	802:803	arg1	shape					819:823	spherical shape	809:823	spherical shape within the EPS matrix	809:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	6	65	from	spectrum	1224:1231	arg1	medicine					1256:1263	medicine	1256:1263	medicine	1256:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	4	66	theme	EPS-Fe	632:637	arg1	complex					639:645	the EPS-Fe complex	628:645	the EPS-Fe complex	628:645	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	6	67	theme	wide	1219:1222	arg1	spectrum					1224:1231	a wide spectrum	1217:1231	a wide spectrum of potential fields in medicine	1217:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	0	68	dep	synthesis	27:35	arg1	supplement					124:133	an oral nano-sized nutritional supplement	93:133	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.	0:166	Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.					
31364687	4	69	theme	NPs	769:771	arg1	distribution					746:757	the uniform distribution	734:757	the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix	734:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	70	theme	magnetic	707:714	arg1	stability					686:694	high thermal stability	673:694	high thermal stability	673:694	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	4	70	theme	magnetic	707:714	arg1	properties					716:725	desirable magnetic properties	697:725	desirable magnetic properties	697:725	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	6	71	theme	suitable	1133:1140	arg1	candidate					1142:1150	a suitable candidate	1131:1150	a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine	1131:1263	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	6	72	theme	organ	1033:1037	arg1	studies					1049:1055	organ histology studies	1033:1055	organ histology studies	1033:1055	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	4	73	theme	spherical	809:817	arg1	shape					819:823	spherical shape	809:823	spherical shape within the EPS matrix	809:845	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
31364687	1	74	theme	Microbial	168:176	arg1	exopolysaccharides					178:195	Microbial exopolysaccharides	168:195	Microbial exopolysaccharides (EPSs)	168:202	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	1	74	theme	Microbial	168:176	arg1	EPSs					198:201	EPSs	198:201	EPSs	198:201	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	6	75	theme	blood	1012:1016	arg1	parameters					1018:1027	Biological blood parameters	1001:1027	Biological blood parameters	1001:1027	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	6	76	theme	Biological	1001:1010	arg1	parameters					1018:1027	Biological blood parameters	1001:1027	Biological blood parameters	1001:1027	Biological blood parameters and organ histology studies confirmed very high safety of the biosynthesized composite, making EPS-Fe a suitable candidate with an economical and environment friendly synthesis method for a wide spectrum of potential fields in medicine.					
31364687	1	77	attach	presence	382:389	arg2	groups					417:422	polyanionic functional groups	394:422	polyanionic functional groups	394:422	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	1	77	attach	presence	382:389	arg1	structure					433:441	their structure	427:441	their structure	427:441	Microbial exopolysaccharides (EPSs) have recently served as an efficient substrate for the production of biocompatible metal nanoparticles (NPs) given their favorable stabilizing and reducing properties due to the presence of polyanionic functional groups in their structure.					
31364687	4	78	theme	complex	639:645	arg1	characterization					608:623	Physicochemical characterization	592:623	Physicochemical characterization of the EPS-Fe complex	592:645	Physicochemical characterization of the EPS-Fe complex was performed, indicating high thermal stability, desirable magnetic properties due to the uniform distribution of the Fe NPs with the average size of ∼10 nm and spherical shape within the EPS matrix.					
30926489	2	0	theme	diet	458:461	arg1	mice					473:476	high fat diet (HFD)-fed mice	449:476	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	4	1	theme	metabolic	673:681	arg1	syndrome					683:690	HFD-induced metabolic syndrome	661:690	HFD-induced metabolic syndrome	661:690	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	2	2	theme	fat	454:456	arg1	HFD					464:466	HFD	464:466	HFD	464:466	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	2	2	theme	fat	454:456	arg1	diet					458:461	high fat diet	449:461	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	1	3	theme	different	158:166	arg1	sources					168:174	different sources	158:174	different sources	158:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	2	4	from	gain	420:423	arg1	mice					473:476	high fat diet (HFD)-fed mice	449:476	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	0	5	theme	fat	88:90	arg1	mice					101:104	high fat diet-fed mice	83:104	high fat diet-fed mice	83:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	0	6	from	Effect	0:5	arg1	activity					71:78	its biological activity	56:78	its biological activity in high fat diet-fed mice	56:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	4	7	theme	specific	801:808	arg1	microbiota					814:823	a specific gut microbiota	799:823	a specific gut microbiota	799:823	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	1	8	theme	polymerization	241:254	arg1	degree					256:261	polymerization degree	241:261	polymerization degree	241:261	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	0	9	theme	high	83:86	arg1	mice					101:104	high fat diet-fed mice	83:104	high fat diet-fed mice	83:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	2	10	from	accumulation	433:444	arg1	mice					473:476	high fat diet (HFD)-fed mice	449:476	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	4	11	theme	nutrient	845:852	arg1	utilization					854:864	nutrient utilization	845:864	nutrient utilization	845:864	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	3	12	theme	intragastric	498:509	arg1	gavage					511:516	intragastric gavage	498:516	intragastric gavage	498:516	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	4	13	from	difference	909:918	arg1	action					938:943	its beneficial action	923:943	its beneficial action	923:943	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	2	14	theme	-fed	468:471	arg1	mice					473:476	high fat diet (HFD)-fed mice	449:476	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	3	15	theme	AGSP	479:482	arg1	treatment					484:492	AGSP treatment	479:492	AGSP treatment via intragastric gavage	479:516	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	3	16	from	parameters	579:588	arg1	comparison					593:602	comparison	593:602	comparison with free diet	593:617	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	2	17	theme	abalone	382:388	arg1	AGSP					397:400	AGSP	397:400	AGSP	397:400	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	2	17	theme	abalone	382:388	arg1	gonad					390:394	abalone gonad	382:394	abalone gonad (AGSP)	382:401	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	0	18	theme	diet-fed	92:99	arg1	mice					101:104	high fat diet-fed mice	83:104	high fat diet-fed mice	83:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	2	19	theme	high	449:452	arg1	HFD					464:466	HFD	464:466	HFD	464:466	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	2	19	theme	high	449:452	arg1	diet					458:461	high fat diet	449:461	high fat diet (HFD)-fed mice	449:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	4	20	theme	beneficial	927:936	arg1	action					938:943	its beneficial action	923:943	its beneficial action	923:943	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	3	21	with	comparison	593:602	arg1	diet					614:617	free diet	609:617	free diet	609:617	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	1	22	from	actions	126:132	arg1	sources					168:174	different sources	158:174	different sources	158:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	4	23	theme	different	633:641	arg1	patterns					643:650	two different patterns	629:650	two different patterns	629:650	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	3	24	theme	better	530:535	arg1	effect					548:553	a better inhibitory effect	528:553	a better inhibitory effect	528:553	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	2	25	theme	sulfated	354:361	arg1	polysaccharides					363:377	sulfated polysaccharides	354:377	sulfated polysaccharides of abalone gonad (AGSP)	354:401	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	2	26	theme	weight	413:418	arg1	gain					420:423	weight gain	413:423	weight gain	413:423	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	5	27	theme	daily	1058:1062	arg1	life					1064:1067	daily life	1058:1067	daily life	1058:1067	Taken together, this study can provide a guidance for the practical application of prebiotic polysaccharides in daily life.					
30926489	0	28	theme	intake	10:15	arg1	pattern					17:23	intake pattern	10:23	intake pattern of sulfated polysaccharides	10:51	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	5	29	theme	prebiotic	1029:1037	arg1	polysaccharides					1039:1053	prebiotic polysaccharides	1029:1053	prebiotic polysaccharides	1029:1053	Taken together, this study can provide a guidance for the practical application of prebiotic polysaccharides in daily life.					
30926489	4	30	theme	HFD-induced	661:671	arg1	syndrome					683:690	HFD-induced metabolic syndrome	661:690	HFD-induced metabolic syndrome	661:690	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	0	31	theme	sulfated	28:35	arg1	polysaccharides					37:51	sulfated polysaccharides	28:51	sulfated polysaccharides	28:51	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	4	32	theme	slight	902:907	arg1	difference					909:918	a slight difference	900:918	a slight difference in its beneficial action	900:943	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	0	33	from	activity	71:78	arg1	mice					101:104	high fat diet-fed mice	83:104	high fat diet-fed mice	83:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	0	34	theme	pattern	17:23	arg1	Effect					0:5	Effect	0:5	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.	0:105	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	5	35	theme	polysaccharides	1039:1053	arg1	application					1014:1024	the practical application	1000:1024	the practical application of prebiotic polysaccharides in daily life	1000:1067	Taken together, this study can provide a guidance for the practical application of prebiotic polysaccharides in daily life.					
30926489	2	36	theme	gonad	390:394	arg1	polysaccharides					363:377	sulfated polysaccharides	354:377	sulfated polysaccharides of abalone gonad (AGSP)	354:401	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	4	37	theme	energy	870:875	arg1	metabolism					877:886	energy metabolism	870:886	energy metabolism	870:886	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	3	38	theme	free	609:612	arg1	diet					614:617	free diet	609:617	free diet	609:617	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	3	39	theme	inhibitory	537:546	arg1	effect					548:553	a better inhibitory effect	528:553	a better inhibitory effect	528:553	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	0	40	theme	polysaccharides	37:51	arg1	pattern					17:23	intake pattern	10:23	intake pattern of sulfated polysaccharides	10:51	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	1	41	theme	intake	290:295	arg1	pattern					297:303	intake pattern	290:303	intake pattern	290:303	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	2	42	theme	fat	429:431	arg1	accumulation					433:444	fat accumulation	429:444	fat accumulation in high fat diet (HFD)-fed mice	429:476	This study showed that sulfated polysaccharides of abalone gonad (AGSP) inhibited weight gain and fat accumulation in high fat diet (HFD)-fed mice.					
30926489	4	43	theme	gut	810:812	arg1	microbiota					814:823	a specific gut microbiota	799:823	a specific gut microbiota	799:823	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	1	44	theme	Various	107:113	arg1	actions					126:132	Various beneficial actions	107:132	Various beneficial actions of polysaccharides from different sources	107:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	5	45	theme	practical	1004:1012	arg1	application					1014:1024	the practical application	1000:1024	the practical application of prebiotic polysaccharides in daily life	1000:1067	Taken together, this study can provide a guidance for the practical application of prebiotic polysaccharides in daily life.					
30926489	4	46	theme	higher	763:768	arg1	levels					780:785	the higher taxonomic levels	759:785	the higher taxonomic levels	759:785	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	1	47	theme	beneficial	115:124	arg1	actions					126:132	Various beneficial actions	107:132	Various beneficial actions of polysaccharides from different sources	107:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	5	48	from	application	1014:1024	arg1	life					1064:1067	daily life	1058:1067	daily life	1058:1067	Taken together, this study can provide a guidance for the practical application of prebiotic polysaccharides in daily life.					
30926489	4	49	from	levels	780:785	arg1	differences					731:741	some differences	726:741	some differences between them at the higher taxonomic levels	726:785	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	4	50	theme	gut	696:698	arg1	dysbiosis					711:719	gut microbiota dysbiosis	696:719	gut microbiota dysbiosis	696:719	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	4	51	theme	microbiota	700:709	arg1	dysbiosis					711:719	gut microbiota dysbiosis	696:719	gut microbiota dysbiosis	696:719	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	0	52	theme	biological	60:69	arg1	activity					71:78	its biological activity	56:78	its biological activity in high fat diet-fed mice	56:104	Effect of intake pattern of sulfated polysaccharides on its biological activity in high fat diet-fed mice.					
30926489	3	53	theme	obesity-related	563:577	arg1	parameters					579:588	some obesity-related parameters	558:588	some obesity-related parameters in comparison with free diet	558:617	AGSP treatment via intragastric gavage exhibited a better inhibitory effect on some obesity-related parameters in comparison with free diet.					
30926489	1	54	theme	active	223:228	arg1	group					230:234	active group	223:234	active group	223:234	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	4	55	theme	taxonomic	770:778	arg1	levels					780:785	the higher taxonomic levels	759:785	the higher taxonomic levels	759:785	AGSP via two different patterns improved HFD-induced metabolic syndrome and gut microbiota dysbiosis, but some differences between them at the higher taxonomic levels resulted in a specific gut microbiota, which could affect nutrient utilization and energy metabolism and lead to a slight difference in its beneficial action.					
30926489	1	56	from	sources	168:174	arg1	polysaccharides					137:151	polysaccharides	137:151	polysaccharides from different sources	137:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	1	56	from	sources	168:174	arg1	actions					126:132	Various beneficial actions	107:132	Various beneficial actions of polysaccharides from different sources	107:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30926489	1	57	theme	polysaccharides	137:151	arg1	actions					126:132	Various beneficial actions	107:132	Various beneficial actions of polysaccharides from different sources	107:174	Various beneficial actions of polysaccharides from different sources have shown to be associated with their dosage, active group, and polymerization degree, but it is unclear whether intake pattern affects their activities.					
30391588	2	0	theme	dilute	533:538	arg1	viscometry					549:558	dilute solution viscometry	533:558	dilute solution viscometry	533:558	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	7	1	theme	grewia	1273:1278	arg1	samples					1280:1286	grewia samples	1273:1286	grewia samples	1273:1286	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	4	2	theme	uronic	875:880	arg1	acids					882:886	uronic acids	875:886	uronic acids	875:886	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	7	3	from	differences	1227:1237	arg1	techniques					1253:1262	extraction techniques	1242:1262	extraction techniques	1242:1262	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	6	4	theme	flow	1130:1133	arg1	behaviour					1135:1143	a shear thinning flow behaviour	1113:1143	a shear thinning flow behaviour	1113:1143	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	1	5	theme	extracts	275:282	arg1	composition					225:235	chemical composition	216:235	chemical composition	216:235	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	1	5	theme	extracts	275:282	arg1	characteristics					252:266	structural characteristics	241:266	structural characteristics	241:266	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	7	6	from	use	1322:1324	arg1	industries					1357:1366	the food and pharmaceutical industries	1329:1366	the food and pharmaceutical industries	1329:1366	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	2	7	theme	FT-IR	399:403	arg1	spectroscopy					413:424	FT-IR and NMR spectroscopy	399:424	spectroscopy	413:424	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	5	8	theme	Intrinsic	949:957	arg1	viscosity					959:967	Intrinsic viscosity	949:967	Intrinsic viscosity	949:967	Intrinsic viscosity varied between 6.5 and 9.1 dL g-1 and related with molar mass (754-2778 × 103 g mol-1).					
30391588	6	9	from	1 g dL-1	1094:1101	arg1	dispersions					1079:1089	Grewia polysaccharide dispersions	1057:1089	Grewia polysaccharide dispersions at 1 g dL-1	1057:1101	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	6	10	theme	polysaccharide	1064:1077	arg1	dispersions					1079:1089	Grewia polysaccharide dispersions	1057:1089	Grewia polysaccharide dispersions at 1 g dL-1	1057:1101	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	2	11	theme	shear	571:575	arg1	rheology					577:584	steady shear rheology	564:584	steady shear rheology	564:584	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	6	12	theme	higher	1198:1203	arg1	viscosities					1205:1215	higher viscosities	1198:1215	higher viscosities	1198:1215	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	2	13	theme	chemical	315:322	arg1	composition					324:334	chemical composition	315:334	chemical composition	315:334	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	1	14	theme	Grewia	65:70	arg1	polysaccharides					72:86	Grewia polysaccharides	65:86	Grewia polysaccharides	65:86	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	6	15	theme	crude	1150:1154	arg1	extracts					1182:1189	crude and sodium metabisulphite extracts	1150:1189	extracts	1182:1189	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	2	16	theme	steady	564:569	arg1	rheology					577:584	steady shear rheology	564:584	steady shear rheology	564:584	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	17	theme	35.1	761:764	arg1	%					765:765	18.5 and 35.1%	752:765	%	765:765	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	2	18	theme	exclusion	456:464	arg1	chromatography					466:479	size exclusion chromatography	451:479	size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS)	451:530	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	2	19	theme	neutral	427:433	arg1	analysis					441:448	neutral sugar analysis	427:448	neutral sugar analysis	427:448	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	1	20	theme	different	177:185	arg1	techniques					198:207	the different extraction techniques	173:207	the different extraction techniques	173:207	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	7	21	theme	extraction	1242:1251	arg1	techniques					1253:1262	extraction techniques	1242:1262	extraction techniques	1242:1262	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	5	22	with	9.1 dL g-1	992:1001	arg1	754-2778 × 103 g mol-1					1032:1053	754-2778 × 103 g mol-1	1032:1053	754-2778 × 103 g mol-1	1032:1053	Intrinsic viscosity varied between 6.5 and 9.1 dL g-1 and related with molar mass (754-2778 × 103 g mol-1).					
30391588	5	22	with	9.1 dL g-1	992:1001	arg1	mass					1026:1029	molar mass	1020:1029	molar mass (754-2778 × 103 g mol-1)	1020:1054	Intrinsic viscosity varied between 6.5 and 9.1 dL g-1 and related with molar mass (754-2778 × 103 g mol-1).					
30391588	3	23	theme	Chemical	587:594	arg1	composition					596:606	Chemical composition	587:606	Chemical composition	587:606	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	1	24	theme	extraction	187:196	arg1	techniques					198:207	the different extraction techniques	173:207	the different extraction techniques	173:207	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	2	25	theme	light	504:508	arg1	SEC-MALS					522:529	SEC-MALS	522:529	SEC-MALS	522:529	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	2	25	theme	light	504:508	arg1	scattering					510:519	multi-angle light scattering	492:519	multi-angle light scattering (SEC-MALS)	492:530	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	26	theme	18.5	752:755	arg1	%					765:765	18.5 and 35.1%	752:765	%	765:765	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	7	27	theme	pharmaceutical	1342:1355	arg1	industries					1357:1366	the food and pharmaceutical industries	1329:1366	the food and pharmaceutical industries	1329:1366	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	2	28	theme	polysaccharide	353:366	arg1	extracts					368:375	the resulting polysaccharide extracts	339:375	the resulting polysaccharide extracts	339:375	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	1	29	theme	techniques	198:207	arg1	influence					160:168	the influence	156:168	the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts	156:282	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	2	30	theme	multi-angle	492:502	arg1	SEC-MALS					522:529	SEC-MALS	522:529	SEC-MALS	522:529	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	2	30	theme	multi-angle	492:502	arg1	scattering					510:519	multi-angle light scattering	492:519	multi-angle light scattering (SEC-MALS)	492:530	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	31	theme	total	771:775	arg1	acid					784:787	total uronic acid	771:787	total uronic acid	771:787	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	2	32	theme	resulting	343:351	arg1	extracts					368:375	the resulting polysaccharide extracts	339:375	the resulting polysaccharide extracts	339:375	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	33	theme	uronic	777:782	arg1	acid					784:787	total uronic acid	771:787	total uronic acid	771:787	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	4	34	theme	esterified	930:939	arg1	groups					941:946	esterified groups	930:946	esterified groups	930:946	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	1	35	from	influence	160:168	arg1	composition					225:235	chemical composition	216:235	chemical composition	216:235	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	1	35	from	influence	160:168	arg1	characteristics					252:266	structural characteristics	241:266	structural characteristics	241:266	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	1	36	theme	sodium	108:113	arg1	metabisulphite					115:128	sodium metabisulphite	108:128	sodium metabisulphite	108:128	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	0	37	theme	physicochemical	14:28	arg1	properties					30:39	physicochemical properties	14:39	physicochemical properties	14:39	Structure and physicochemical properties of Ghanaian grewia gum.					
30391588	6	38	theme	thinning	1121:1128	arg1	behaviour					1135:1143	a shear thinning flow behaviour	1113:1143	a shear thinning flow behaviour	1113:1143	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	1	39	theme	chemical	216:223	arg1	composition					225:235	chemical composition	216:235	chemical composition	216:235	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	0	40	theme	Ghanaian	44:51	arg1	gum					60:62	Ghanaian grewia gum	44:62	Ghanaian grewia gum	44:62	Structure and physicochemical properties of Ghanaian grewia gum.					
30391588	6	41	theme	sodium	1160:1165	arg1	extracts					1182:1189	crude and sodium metabisulphite extracts	1150:1189	extracts	1182:1189	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	4	42	dep	Predominate	822:832	arg1	rhamnose					862:869	rhamnose	862:869	rhamnose	862:869	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	4	42	dep	Predominate	822:832	arg1	acids					882:886	uronic acids	875:886	uronic acids	875:886	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	2	43	theme	size	451:454	arg1	chromatography					466:479	size exclusion chromatography	451:479	size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS)	451:530	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	6	44	theme	shear	1115:1119	arg1	behaviour					1135:1143	a shear thinning flow behaviour	1113:1143	a shear thinning flow behaviour	1113:1143	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	3	45	theme	23.5	793:796	arg1	%					806:806	23.5 and 28.6%	793:806	%	806:806	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	4	46	theme	groups	941:946	arg1	presence					918:925	the presence	914:925	the presence of esterified groups	914:946	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	6	47	theme	Grewia	1057:1062	arg1	dispersions					1079:1089	Grewia polysaccharide dispersions	1057:1089	Grewia polysaccharide dispersions at 1 g dL-1	1057:1101	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	0	48	theme	gum	60:62	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and physicochemical properties of Ghanaian grewia gum.					
30391588	0	48	theme	gum	60:62	arg1	properties					30:39	physicochemical properties	14:39	physicochemical properties	14:39	Structure and physicochemical properties of Ghanaian grewia gum.					
30391588	7	49	theme	tailored	1293:1300	arg1	properties					1307:1316	tailored bulk properties	1293:1316	tailored bulk properties for use in the food and pharmaceutical industries	1293:1366	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	3	50	theme	extraction	642:651	arg1	solvent					653:659	the extraction solvent	638:659	the extraction solvent used	638:664	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	4	51	from	sugars	834:839	arg1	extracts					848:855	the extracts	844:855	the extracts	844:855	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	6	52	theme	metabisulphite	1167:1180	arg1	extracts					1182:1189	crude and sodium metabisulphite extracts	1150:1189	extracts	1182:1189	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	0	53	theme	grewia	53:58	arg1	gum					60:62	Ghanaian grewia gum	44:62	Ghanaian grewia gum	44:62	Structure and physicochemical properties of Ghanaian grewia gum.					
30391588	3	54	theme	solvent	653:659	arg1	similar					612:618	similar	612:618	similar	612:618	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	7	55	theme	food	1333:1336	arg1	industries					1357:1366	the food and pharmaceutical industries	1329:1366	the food and pharmaceutical industries	1329:1366	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	1	56	theme	phosphate	134:142	arg1	buffers					144:150	phosphate buffers	134:150	phosphate buffers	134:150	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	7	57	theme	bulk	1302:1305	arg1	properties					1307:1316	tailored bulk properties	1293:1316	tailored bulk properties for use in the food and pharmaceutical industries	1293:1366	Overall, differences in extraction techniques produced grewia samples with tailored bulk properties for use in the food and pharmaceutical industries.					
30391588	6	58	contain	having	1191:1196	arg1	extracts					1182:1189	crude and sodium metabisulphite extracts	1150:1189	extracts	1182:1189	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	6	58	contain	having	1191:1196	arg2	viscosities					1205:1215	higher viscosities	1198:1215	higher viscosities	1198:1215	Grewia polysaccharide dispersions at 1 g dL-1 exhibited a shear thinning flow behaviour with crude and sodium metabisulphite extracts having higher viscosities.					
30391588	1	59	dep	composition	225:235	arg1	the					212:214	the	212:214	the	212:214	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	4	60	with	acids	882:886	arg1	spectroscopy					893:904	spectroscopy	893:904	spectroscopy showing the presence of esterified groups	893:946	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
30391588	1	61	theme	structural	241:250	arg1	characteristics					252:266	structural characteristics	241:266	structural characteristics	241:266	Grewia polysaccharides were isolated using sodium metabisulphite and phosphate buffers and the influence of the different extraction techniques on the chemical composition and structural characteristics of the extracts were determined.					
30391588	2	62	theme	extracts	368:375	arg1	Structure					301:309	Structure	301:309	Structure	301:309	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	2	62	theme	extracts	368:375	arg1	composition					324:334	chemical composition	315:334	chemical composition	315:334	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	5	63	theme	molar	1020:1024	arg1	754-2778 × 103 g mol-1					1032:1053	754-2778 × 103 g mol-1	1032:1053	754-2778 × 103 g mol-1	1032:1053	Intrinsic viscosity varied between 6.5 and 9.1 dL g-1 and related with molar mass (754-2778 × 103 g mol-1).					
30391588	5	63	theme	molar	1020:1024	arg1	mass					1026:1029	molar mass	1020:1029	molar mass (754-2778 × 103 g mol-1)	1020:1054	Intrinsic viscosity varied between 6.5 and 9.1 dL g-1 and related with molar mass (754-2778 × 103 g mol-1).					
30391588	2	64	theme	sugar	435:439	arg1	analysis					441:448	neutral sugar analysis	427:448	neutral sugar analysis	427:448	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	65	theme	28.6	802:805	arg1	%					806:806	23.5 and 28.6%	793:806	%	806:806	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	2	66	theme	solution	540:547	arg1	viscometry					549:558	dilute solution viscometry	533:558	dilute solution viscometry	533:558	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	3	67	theme	total	732:736	arg1	carbohydrate					738:749	total carbohydrate	732:749	total carbohydrate	732:749	Chemical composition was similar irrespectively of the extraction solvent used and ranged between 11.1 and 16.5% for protein, 53.4 and 66.9% for total carbohydrate, 18.5 and 35.1% for total uronic acid and 23.5 and 28.6% for rhamnose.					
30391588	2	68	theme	NMR	409:411	arg1	spectroscopy					413:424	FT-IR and NMR spectroscopy	399:424	spectroscopy	413:424	Structure and chemical composition of the resulting polysaccharide extracts were determined using FT-IR and NMR spectroscopy, neutral sugar analysis, size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), dilute solution viscometry and steady shear rheology.					
30391588	4	69	with	rhamnose	862:869	arg1	spectroscopy					893:904	spectroscopy	893:904	spectroscopy showing the presence of esterified groups	893:946	Predominate sugars in the extracts were rhamnose and uronic acids with spectroscopy showing the presence of esterified groups.					
31151236	0	0	theme	Improved	84:91	arg1	Composite					105:113	Mechanically Improved Electrospun Composite	71:113	Mechanically Improved Electrospun Composite	71:113	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	0	1	from	Interaction	20:30	arg1	Composite					105:113	Mechanically Improved Electrospun Composite	71:113	Mechanically Improved Electrospun Composite	71:113	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	5	2	theme	intra-molecular	1157:1171	arg1	bonds					1182:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	0	3	theme	Mechanically	71:82	arg1	Composite					105:113	Mechanically Improved Electrospun Composite	71:113	Mechanically Improved Electrospun Composite	71:113	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	4	4	theme	homogeneous	922:932	arg1	phase					934:938	a relatively homogeneous phase	909:938	a relatively homogeneous phase	909:938	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	1	5	theme	regenerative	171:182	arg1	medicine					184:191	regenerative medicine	171:191	regenerative medicine	171:191	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	1	6	theme	insufficient	267:278	arg1	issue					211:215	the most elusive issue	194:215	the most elusive issue in the collagen-based biomedical scaffolds	194:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	1	6	theme	insufficient	267:278	arg1	strength					291:298	its insufficient mechanical strength	263:298	its insufficient mechanical strength	263:298	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	2	7	from	composites	467:476	arg1	cause					424:428	the cause	420:428	the cause of the mechanical improvement in the composites	420:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	7	from	composites	467:476	arg1	interactions					397:408	molecular interactions	387:408	molecular interactions which are the cause of the mechanical improvement in the composites	387:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	5	8	dep	bonds	1182:1186	arg1	i.e.					1132:1135	i.e.	1132:1135	i.e.	1132:1135	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	5	9	theme	higher	1014:1019	arg1	strength					1021:1028	~41% and ~14% higher strength	1000:1028	~41% and ~14% higher strength	1000:1028	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	6	10	theme	revealed	1193:1200	arg1	interaction					1212:1222	The revealed molecular interaction	1189:1222	The revealed molecular interaction	1189:1222	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	1	11	theme	mechanical	280:289	arg1	issue					211:215	the most elusive issue	194:215	the most elusive issue in the collagen-based biomedical scaffolds	194:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	1	11	theme	mechanical	280:289	arg1	strength					291:298	its insufficient mechanical strength	263:298	its insufficient mechanical strength	263:298	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	0	12	theme	Electrospun	93:103	arg1	Composite					105:113	Mechanically Improved Electrospun Composite	71:113	Mechanically Improved Electrospun Composite	71:113	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	5	13	theme	inter-	1138:1143	arg1	bonds					1182:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	6	14	theme	hydrogen	1280:1287	arg1	bonds					1289:1293	inter-molecular hydrogen bonds	1264:1293	inter-molecular hydrogen bonds with collagen	1264:1307	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	1	15	used	used	163:166	arg2	collagens					125:133	collagens	125:133	collagens from vertebrates	125:150	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	6	16	theme	crystalline	1341:1351	arg1	regions					1353:1359	their uncrumpled crystalline regions	1324:1359	their uncrumpled crystalline regions	1324:1359	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	1	17	from	vertebrates	140:150	arg1	collagens					125:133	collagens	125:133	collagens from vertebrates	125:150	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	0	18	theme	Molecular	10:18	arg1	Interaction					20:30	Different Molecular Interaction	0:30	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.	0:114	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	3	19	from	configurations	725:738	arg1	structure					755:763	the crystal structure	743:763	the crystal structure	743:763	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	6	20	theme	uncrumpled	1330:1339	arg1	regions					1353:1359	their uncrumpled crystalline regions	1324:1359	their uncrumpled crystalline regions	1324:1359	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	2	21	theme	collagen	336:343	arg1	composites					345:354	electrospun collagen composites	324:354	electrospun collagen composites with chitins	324:367	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	0	22	theme	Different	0:8	arg1	Interaction					20:30	Different Molecular Interaction	0:30	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.	0:114	Different Molecular Interaction between Collagen and α- or β-Chitin in Mechanically Improved Electrospun Composite.					
31151236	5	23	theme	secondary	1109:1117	arg1	interaction					1119:1129	a favorable secondary interaction	1097:1129	a favorable secondary interaction	1097:1129	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	2	24	theme	electrospun	324:334	arg1	composites					345:354	electrospun collagen composites	324:354	electrospun collagen composites with chitins	324:367	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	6	25	theme	inter-molecular	1264:1278	arg1	bonds					1289:1293	inter-molecular hydrogen bonds	1264:1293	inter-molecular hydrogen bonds with collagen	1264:1307	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	6	26	theme	molecular	1202:1210	arg1	interaction					1212:1222	The revealed molecular interaction	1189:1222	The revealed molecular interaction	1189:1222	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	2	27	theme	improvement	448:458	arg1	cause					424:428	the cause	420:428	the cause of the mechanical improvement in the composites	420:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	27	theme	improvement	448:458	arg1	interactions					397:408	molecular interactions	387:408	molecular interactions which are the cause of the mechanical improvement in the composites	387:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	5	28	theme	better	971:976	arg1	strength					986:993	better tensile strength	971:993	better tensile strength	971:993	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	2	29	theme	mechanical	437:446	arg1	improvement					448:458	the mechanical improvement	433:458	the mechanical improvement in the composites	433:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	30	theme	correlation	515:525	arg1	2DCOS					541:545	2DCOS	541:545	2DCOS	541:545	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	30	theme	correlation	515:525	arg1	spectroscopy					527:538	two-dimensional correlation spectroscopy	499:538	two-dimensional correlation spectroscopy (2DCOS)	499:546	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	3	31	theme	polymer	791:797	arg1	susceptibility					799:812	polymer susceptibility	791:812	polymer susceptibility	791:812	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	2	32	with	composites	345:354	arg1	chitins					361:367	chitins	361:367	chitins	361:367	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	33	theme	two-dimensional	499:513	arg1	2DCOS					541:545	2DCOS	541:545	2DCOS	541:545	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	33	theme	two-dimensional	499:513	arg1	spectroscopy					527:538	two-dimensional correlation spectroscopy	499:538	two-dimensional correlation spectroscopy (2DCOS)	499:546	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	4	34	contain	has	837:839	arg1	collagen/α-chitin					819:835	The collagen/α-chitin	815:835	The collagen/α-chitin	815:835	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	4	34	contain	has	837:839	arg2	phases					857:862	two distinctive phases	841:862	two distinctive phases	841:862	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	4	35	dep	composite	871:879	arg1	the					867:869	the	867:869	the	867:869	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	5	36	theme	β-chitin	945:952	arg1	composite					954:962	The β-chitin composite	941:962	The β-chitin composite	941:962	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	3	37	theme	molecular	676:684	arg1	interactions					686:697	molecular interactions	676:697	molecular interactions	676:697	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	4	38	theme	β-chitin	886:893	arg1	composite					895:903	β-chitin composite	886:903	β-chitin composite	886:903	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	5	39	theme	tensile	978:984	arg1	strength					986:993	better tensile strength	971:993	better tensile strength	971:993	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	5	40	theme	hydrogen	1173:1180	arg1	bonds					1182:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	inter- rather than intra-molecular hydrogen bonds	1138:1186	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	3	41	theme	inherent	716:723	arg1	configurations					725:738	different inherent configurations	706:738	different inherent configurations in the crystal structure	706:763	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	5	42	theme	due	1090:1092	arg1	collagen					1042:1049	collagen	1042:1049	collagen	1042:1049	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	2	43	from	cause	424:428	arg1	composites					467:476	the composites	463:476	the composites	463:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	3	44	theme	polymorphs	602:611	arg1	kinds					593:597	two kinds	589:597	two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility	589:812	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	3	45	theme	mechanical	649:658	arg1	enhancement					660:670	different mechanical enhancement	639:670	different mechanical enhancement	639:670	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	1	46	from	issue	211:215	arg1	scaffolds					250:258	the collagen-based biomedical scaffolds	220:258	the collagen-based biomedical scaffolds	220:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	1	47	theme	collagen-based	224:237	arg1	scaffolds					250:258	the collagen-based biomedical scaffolds	220:258	the collagen-based biomedical scaffolds	220:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	5	48	theme	favorable	1099:1107	arg1	interaction					1119:1129	a favorable secondary interaction	1097:1129	a favorable secondary interaction	1097:1129	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	4	49	theme	distinctive	845:855	arg1	phases					857:862	two distinctive phases	841:862	two distinctive phases	841:862	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	1	50	theme	biomedical	239:248	arg1	scaffolds					250:258	the collagen-based biomedical scaffolds	220:258	the collagen-based biomedical scaffolds	220:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	3	51	theme	different	706:714	arg1	configurations					725:738	different inherent configurations	706:738	different inherent configurations in the crystal structure	706:763	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	5	52	theme	α-chitin	1055:1062	arg1	composites					1064:1073	α-chitin composites	1055:1073	α-chitin composites	1055:1073	The β-chitin composite showed better tensile strength with ~41% and ~14% higher strength compared to collagen and α-chitin composites, respectively, due to a favorable secondary interaction, i.e., inter- rather than intra-molecular hydrogen bonds.					
31151236	1	53	theme	elusive	203:209	arg1	issue					211:215	the most elusive issue	194:215	the most elusive issue in the collagen-based biomedical scaffolds	194:258	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	1	53	theme	elusive	203:209	arg1	strength					291:298	its insufficient mechanical strength	263:298	its insufficient mechanical strength	263:298	Although collagens from vertebrates are mainly used in regenerative medicine, the most elusive issue in the collagen-based biomedical scaffolds is its insufficient mechanical strength.					
31151236	3	54	theme	electrospun	553:563	arg1	collagen					565:572	The electrospun collagen	549:572	The electrospun collagen	549:572	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	4	55	contain	has	905:907	arg1	composite					895:903	β-chitin composite	886:903	β-chitin composite	886:903	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	4	55	contain	has	905:907	arg2	phase					934:938	a relatively homogeneous phase	909:938	a relatively homogeneous phase	909:938	The collagen/α-chitin has two distinctive phases in the composite, but β-chitin composite has a relatively homogeneous phase.					
31151236	6	56	with	bonds	1289:1293	arg1	collagen					1300:1307	collagen	1300:1307	collagen	1300:1307	The revealed molecular interaction indicates that β-chitin prefers to form inter-molecular hydrogen bonds with collagen by rearranging their uncrumpled crystalline regions, unlike α-chitin.					
31151236	3	57	theme	crystal	747:753	arg1	structure					755:763	the crystal structure	743:763	the crystal structure	743:763	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	3	58	theme	different	639:647	arg1	enhancement					660:670	different mechanical enhancement	639:670	different mechanical enhancement	639:670	The electrospun collagen is composed of two kinds of polymorphs, α- and β-chitin, showing different mechanical enhancement and molecular interactions due to different inherent configurations in the crystal structure, resulting in solvent and polymer susceptibility.					
31151236	2	59	from	improvement	448:458	arg1	composites					467:476	the composites	463:476	the composites	463:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	60	theme	molecular	387:395	arg1	cause					424:428	the cause	420:428	the cause of the mechanical improvement in the composites	420:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31151236	2	60	theme	molecular	387:395	arg1	interactions					397:408	molecular interactions	387:408	molecular interactions which are the cause of the mechanical improvement in the composites	387:476	To solve this problem, electrospun collagen composites with chitins were prepared and molecular interactions which are the cause of the mechanical improvement in the composites were investigated by two-dimensional correlation spectroscopy (2DCOS).					
31022848	7	0	theme	reactive	1199:1206	arg1	ROS					1224:1226	ROS	1224:1226	ROS	1224:1226	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	7	0	theme	reactive	1199:1206	arg1	species					1215:1221	reactive oxygen species	1199:1221	reactive oxygen species (ROS)	1199:1227	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	5	1	theme	chromatography-refractive	869:893	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	1	theme	chromatography-refractive	869:893	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	8	2	theme	potential	1392:1400	arg1	agents					1402:1407	potential agents	1392:1407	potential agents in the fields of functional foods or medicine	1392:1453	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	8	2	theme	potential	1392:1400	arg1	-β-d-glucans					1334:1345	the (1→6)-β-d-glucans	1325:1345	the (1→6)-β-d-glucans obtained from Lentinus edodes	1325:1375	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	5	3	theme	laser	913:917	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	3	theme	laser	913:917	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	7	4	theme	oxygen	1208:1213	arg1	ROS					1224:1226	ROS	1224:1226	ROS	1224:1226	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	7	4	theme	oxygen	1208:1213	arg1	species					1215:1221	reactive oxygen species	1199:1221	reactive oxygen species (ROS)	1199:1227	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	7	5	theme	RAW	1115:1117	arg1	activation					1136:1145	RAW 264.7 macrophage activation	1115:1145	RAW 264.7 macrophage activation	1115:1145	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	3	6	with	purification	504:515	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	5	7	theme	light	919:923	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	7	theme	light	919:923	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	3	8	theme	Flow	541:544	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	0	9	theme	-β-d-Glucan	76:86	arg1	Extraction					9:18	Alkaline Extraction	0:18	Alkaline Extraction	0:18	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	0	9	theme	-β-d-Glucan	76:86	arg1	Characterization					32:47	Structural Characterization	21:47	Structural Characterization	21:47	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	0	9	theme	-β-d-Glucan	76:86	arg1	Bioactivities					54:66	Bioactivities	54:66	Bioactivities	54:66	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	5	10	theme	compact	987:993	arg1	unit					995:998	a compact unit	985:998	a compact unit in aqueous solution	985:1018	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	3	11	theme	strong	546:551	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	1	12	theme	structural	235:244	arg1	properties					261:270	their structural and biological properties	229:270	their structural and biological properties for sustainable utilization	229:298	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	5	13	theme	force	810:814	arg1	AFM					828:830	AFM	828:830	AFM	828:830	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	13	theme	force	810:814	arg1	microscopy					816:825	Atomic force microscopy	803:825	Atomic force microscopy (AFM)	803:831	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	3	14	theme	Q-Sepharose	524:534	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	5	15	theme	gel	854:856	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	15	theme	gel	854:856	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	4	16	theme	side	790:793	arg1	chains					795:800	minor β-(1→3) glucosidic side chains	765:800	minor β-(1→3) glucosidic side chains	765:800	LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains.					
31022848	0	17	dep	-β-d-Glucan	76:86	arg1	1→6					72:74	1→6	72:74	1→6	72:74	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	5	18	theme	high-performance	837:852	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	18	theme	high-performance	837:852	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	7	19	theme	nitric	1177:1182	arg1	NO					1191:1192	NO	1191:1192	NO	1191:1192	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	7	19	theme	nitric	1177:1182	arg1	oxide					1184:1188	nitric oxide	1177:1188	nitric oxide (NO)	1177:1193	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	8	20	from	agents	1402:1407	arg1	fields					1416:1421	the fields	1412:1421	the fields of functional foods or medicine	1412:1453	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	4	21	theme	1→6	743:745	arg1	-β-d-glucans					747:758	(1→6)-β-d-glucans	742:758	(1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains	742:800	LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains.					
31022848	1	22	theme	robust	152:157	arg1	approach					159:166	a robust approach	150:166	a robust approach to obtain β glucans from Lentinus edodes	150:207	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	0	23	theme	Alkaline	0:7	arg1	Extraction					9:18	Alkaline Extraction	0:18	Alkaline Extraction	0:18	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	8	24	theme	foods	1437:1441	arg1	fields					1416:1421	the fields	1412:1421	the fields of functional foods or medicine	1412:1453	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	8	25	theme	medicine	1446:1453	arg1	fields					1416:1421	the fields	1412:1421	the fields of functional foods or medicine	1412:1453	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	8	26	theme	functional	1426:1435	arg1	foods					1437:1441	functional foods	1426:1441	functional foods	1426:1441	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	5	27	theme	permeation	858:867	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	27	theme	permeation	858:867	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	0	28	theme	Structural	21:30	arg1	Characterization					32:47	Structural Characterization	21:47	Structural Characterization	21:47	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	1	29	theme	biological	250:259	arg1	properties					261:270	their structural and biological properties	229:270	their structural and biological properties for sustainable utilization	229:298	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	8	30	theme	Lentinus	1361:1368	arg1	edodes					1370:1375	Lentinus edodes	1361:1375	Lentinus edodes	1361:1375	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	3	31	theme	anion-exchange	553:566	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	6	32	theme	antioxidant	1058:1068	arg1	activities					1070:1079	antioxidant activities	1058:1079	antioxidant activities with IC50 at 157 μg/mL	1058:1102	This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL.					
31022848	2	33	theme	specific	413:420	arg1	polysaccharides					432:446	specific targeting polysaccharides	413:446	specific targeting polysaccharides	413:446	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	6	34	theme	157	1094:1096	arg1	μg/mL					1098:1102	157 μg/mL	1094:1102	157 μg/mL	1094:1102	This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL.					
31022848	8	35	theme	1→6	1330:1332	arg1	agents					1402:1407	potential agents	1392:1407	potential agents in the fields of functional foods or medicine	1392:1453	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	8	35	theme	1→6	1330:1332	arg1	-β-d-glucans					1334:1345	the (1→6)-β-d-glucans	1325:1345	the (1→6)-β-d-glucans obtained from Lentinus edodes	1325:1375	These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.					
31022848	5	36	theme	Atomic	803:808	arg1	AFM					828:830	AFM	828:830	AFM	828:830	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	36	theme	Atomic	803:808	arg1	microscopy					816:825	Atomic force microscopy	803:825	Atomic force microscopy (AFM)	803:831	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	6	37	with	activities	1070:1079	arg1	IC50					1086:1089	IC50	1086:1089	IC50 at 157 μg/mL	1086:1102	This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL.					
31022848	7	38	theme	μg/mL	1290:1294	arg1	dose					1278:1281	a dose	1276:1281	a dose of 100 μg/mL	1276:1294	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	1	39	theme	β	178:178	arg1	glucans					180:186	β glucans	178:186	β glucans from Lentinus edodes	178:207	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	1	40	theme	sustainable	276:286	arg1	utilization					288:298	sustainable utilization	276:298	sustainable utilization	276:298	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	3	41	theme	NMR	636:638	arg1	spectroscopy					640:651	NMR spectroscopy	636:651	NMR spectroscopy	636:651	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	2	42	theme	targeting	422:430	arg1	polysaccharides					432:446	specific targeting polysaccharides	413:446	specific targeting polysaccharides	413:446	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	2	43	theme	concise	383:389	arg1	process					391:397	a concise process	381:397	a concise process for obtaining specific targeting polysaccharides from Lentinus edodes	381:467	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	7	44	theme	macrophage	1125:1134	arg1	activation					1136:1145	RAW 264.7 macrophage activation	1115:1145	RAW 264.7 macrophage activation	1115:1145	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	4	45	with	-β-d-glucans	747:758	arg1	chains					795:800	minor β-(1→3) glucosidic side chains	765:800	minor β-(1→3) glucosidic side chains	765:800	LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains.					
31022848	1	46	from	edodes	202:207	arg1	glucans					180:186	β glucans	178:186	β glucans from Lentinus edodes	178:207	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	3	47	theme	monosaccharide	580:593	arg1	composition					595:605	the monosaccharide composition	576:605	the monosaccharide composition	576:605	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	2	48	theme	Lentinus	453:460	arg1	edodes					462:467	Lentinus edodes	453:467	Lentinus edodes	453:467	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	3	49	theme	structural	677:686	arg1	characterizations					688:704	their structural characterizations	671:704	their structural characterizations	671:704	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	1	50	theme	study	130:134	arg1	purpose					114:120	The purpose	110:120	The purpose of this study	110:134	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	7	51	from	dose	1278:1281	arg1	cytotoxicity					1260:1271	no cytotoxicity	1257:1271	no cytotoxicity at a dose of 100 μg/mL	1257:1294	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	7	52	theme	oxide	1184:1188	arg1	release					1166:1172	the release	1162:1172	the release of nitric oxide (NO) and reactive oxygen species (ROS)	1162:1227	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	0	53	from	Lentinus	93:100	arg1	Extraction					9:18	Alkaline Extraction	0:18	Alkaline Extraction	0:18	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	0	53	from	Lentinus	93:100	arg1	Characterization					32:47	Structural Characterization	21:47	Structural Characterization	21:47	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	0	53	from	Lentinus	93:100	arg1	Bioactivities					54:66	Bioactivities	54:66	Bioactivities	54:66	Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes.					
31022848	5	54	theme	aqueous	1003:1009	arg1	solution					1011:1018	aqueous solution	1003:1018	aqueous solution	1003:1018	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	3	55	theme	Fast	536:539	arg1	column					568:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	a Q-Sepharose Fast Flow strong anion-exchange column	522:573	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	4	56	theme	minor	765:769	arg1	chains					795:800	minor β-(1→3) glucosidic side chains	765:800	minor β-(1→3) glucosidic side chains	765:800	LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains.					
31022848	6	57	from	μg/mL	1098:1102	arg1	IC50					1086:1089	IC50	1086:1089	IC50 at 157 μg/mL	1086:1102	This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL.					
31022848	2	58	theme	alkali	305:310	arg1	extraction					312:321	The alkali extraction	301:321	The alkali extraction	301:321	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	1	59	theme	Lentinus	193:200	arg1	edodes					202:207	Lentinus edodes	193:207	Lentinus edodes	193:207	The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization.					
31022848	7	60	theme	species	1215:1221	arg1	release					1166:1172	the release	1162:1172	the release of nitric oxide (NO) and reactive oxygen species (ROS)	1162:1227	Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL.					
31022848	5	61	theme	index-multi-angle	895:911	arg1	HPGPC-RI-MALLS					937:950	HPGPC-RI-MALLS	937:950	HPGPC-RI-MALLS	937:950	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	5	61	theme	index-multi-angle	895:911	arg1	scattering					925:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering	837:934	high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS)	837:951	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31022848	3	62	theme	methylation	610:620	arg1	analysis					622:629	a methylation analysis	608:629	a methylation analysis	608:629	After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations.					
31022848	2	63	theme	experimental	356:367	arg1	design					369:374	an orthogonal experimental design	342:374	an orthogonal experimental design	342:374	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	6	64	theme	1→6	1027:1029	arg1	-β-d-glucan					1031:1041	This (1→6)-β-d-glucan	1021:1041	This (1→6)-β-d-glucan	1021:1041	This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL.					
31022848	4	65	theme	glucosidic	779:788	arg1	chains					795:800	minor β-(1→3) glucosidic side chains	765:800	minor β-(1→3) glucosidic side chains	765:800	LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains.					
31022848	2	66	theme	orthogonal	345:354	arg1	design					369:374	an orthogonal experimental design	342:374	an orthogonal experimental design	342:374	The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study.					
31022848	5	67	from	unit	995:998	arg1	solution					1011:1018	aqueous solution	1003:1018	aqueous solution	1003:1018	Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution.					
31071403	0	0	theme	sulfate	97:103	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	0	0	theme	sulfate	97:103	arg1	analysis					28:35	compositional analysis	14:35	compositional analysis	14:35	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	0	0	theme	sulfate	97:103	arg1	capacity					56:63	anticoagulant capacity	42:63	anticoagulant capacity	42:63	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	1	1	theme	chemical	285:292	arg1	composition					294:304	chemical composition	285:304	chemical composition	285:304	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	1	2	theme	sulfate/dermatan	152:167	arg1	CS/DS					178:182	CS/DS	178:182	CS/DS	178:182	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	1	2	theme	sulfate/dermatan	152:167	arg1	sulfate					169:175	Chondroitin sulfate/dermatan sulfate	140:175	Chondroitin sulfate/dermatan sulfate (CS/DS)	140:183	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	0	3	theme	sulfate/dermatan	80:95	arg1	sulfate					97:103	chondroitin sulfate/dermatan sulfate	68:103	chondroitin sulfate/dermatan sulfate	68:103	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	3	4	dep	sulfate	534:540	arg1	%					532:532	%	532:532	%	532:532	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	4	5	from	disaccharides	656:668	arg1	disaccharides					766:778	disulfated disaccharides	755:778	disulfated disaccharides	755:778	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	5	from	disaccharides	656:668	arg1	positions					673:681	positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides	673:778	positions	673:681	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	10	6	theme	soothing	1526:1533	arg1	potential					1482:1490	a promising anticoagulant potential	1456:1490	a promising anticoagulant potential	1456:1490	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	10	6	theme	soothing	1526:1533	arg1	drug					1535:1538	a novel and soothing drug	1514:1538	a novel and soothing drug	1514:1538	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	3	7	theme	%	510:510	arg1	yield					499:503	a yield	497:503	a yield of 10%	497:510	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	8	8	from	concentration	1309:1321	arg1	control					1296:1302	the negative control	1283:1302	the negative control at a concentration of 1000 μg/mL	1283:1335	In fact, CBG prolonged significantly (p < 0.05), aPTT and PT about 2.62 and 1.26 fold, respectively, greater than that of the negative control at a concentration of 1000 μg/mL.					
31071403	1	9	theme	anticoagulant	310:322	arg1	activity					324:331	anticoagulant activity	310:331	anticoagulant activity	310:331	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	7	10	theme	corb	1023:1026	arg1	bone					1015:1018	bone	1015:1018	bone of corb	1015:1026	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	3	11	dep	%	532:532	arg1	31.28					527:531	31.28	527:531	31.28	527:531	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	6	12	theme	purified	925:932	arg1	CS/DS					934:938	the purified CS/DS	921:938	the purified CS/DS	921:938	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	0	13	theme	corb	118:121	arg1	bone					110:113	bone	110:113	bone of corb (Sciaena umbra)	110:137	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	5	14	theme	sulfated	854:861	arg1	residues					863:870	4:6 sulfated residues	850:870	4:6 sulfated residues	850:870	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	9	15	theme	3.53	1377:1380	arg1	fold					1382:1385	prolonged 3.53 fold	1367:1385	prolonged 3.53 fold	1367:1385	However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL.					
31071403	10	16	theme	anticoagulant	1468:1480	arg1	potential					1482:1490	a promising anticoagulant potential	1456:1490	a promising anticoagulant potential	1456:1490	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	10	16	theme	anticoagulant	1468:1480	arg1	drug					1535:1538	a novel and soothing drug	1514:1538	a novel and soothing drug	1514:1538	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	7	17	theme	anticoagulant	1044:1056	arg1	effect					1058:1063	a strong anticoagulant effect	1035:1063	a strong anticoagulant effect	1035:1063	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	10	18	used	used	1506:1509	arg2	drug					1535:1538	a novel and soothing drug	1514:1538	a novel and soothing drug	1514:1538	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	10	18	used	used	1506:1509	arg2	potential					1482:1490	a promising anticoagulant potential	1456:1490	a promising anticoagulant potential	1456:1490	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	7	19	theme	thrombosis	1091:1100	arg1	aPTT					1108:1111	aPTT	1108:1111	aPTT	1108:1111	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	7	19	theme	thrombosis	1091:1100	arg1	time					1102:1105	activated partial thrombosis time	1073:1105	activated partial thrombosis time (aPTT)	1073:1112	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	4	20	theme	N-acetylgalactosamine	698:718	arg1	N-acetylgalactosamine					698:718	the N-acetylgalactosamine	694:718	the N-acetylgalactosamine (8.6% and 40.0%, respectively)	694:749	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	20	theme	N-acetylgalactosamine	698:718	arg1	6					683:683	6	683:683	6	683:683	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	2	21	theme	Infrared	348:355	arg1	spectrum					357:364	Infrared spectrum	348:364	Infrared spectrum	348:364	Infrared spectrum and agarose-gel electrophoresis for extracted CS/DS were also investigated.					
31071403	10	22	theme	purified	1431:1438	arg1	CS/DS					1440:1444	The purified CS/DS	1427:1444	The purified CS/DS	1427:1444	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	9	23	theme	prolonged	1367:1375	arg1	fold					1382:1385	prolonged 3.53 fold	1367:1385	prolonged 3.53 fold	1367:1385	However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL.					
31071403	7	24	theme	activated	1073:1081	arg1	aPTT					1108:1111	aPTT	1108:1111	aPTT	1108:1111	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	7	24	theme	activated	1073:1081	arg1	time					1102:1105	activated partial thrombosis time	1073:1105	activated partial thrombosis time (aPTT)	1073:1112	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	6	25	contain	contained	940:948	arg2	CS					961:962	74% CS	957:962	74% CS	957:962	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	6	25	contain	contained	940:948	arg1	CS/DS					934:938	the purified CS/DS	921:938	the purified CS/DS	921:938	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	6	25	contain	contained	940:948	arg2	DS					972:973	26% DS	968:973	26% DS	968:973	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	2	26	theme	extracted	402:410	arg1	CS/DS					412:416	extracted CS/DS	402:416	extracted CS/DS	402:416	Infrared spectrum and agarose-gel electrophoresis for extracted CS/DS were also investigated.					
31071403	0	27	theme	compositional	14:26	arg1	analysis					28:35	compositional analysis	14:35	compositional analysis	14:35	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	7	28	theme	new	990:992	arg1	CS/DS					994:998	the new CS/DS	986:998	the new CS/DS extracted from bone of corb	986:1026	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	5	29	theme	4:6	850:852	arg1	residues					863:870	4:6 sulfated residues	850:870	4:6 sulfated residues	850:870	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	9	30	theme	CBG	1359:1361	arg1	assay					1350:1354	TT assay	1347:1354	TT assay of CBG	1347:1361	However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL.					
31071403	4	31	theme	Disaccharide	586:597	arg1	analysis					599:606	Disaccharide analysis	586:606	Disaccharide analysis	586:606	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	10	32	theme	novel	1516:1520	arg1	potential					1482:1490	a promising anticoagulant potential	1456:1490	a promising anticoagulant potential	1456:1490	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	10	32	theme	novel	1516:1520	arg1	drug					1535:1538	a novel and soothing drug	1514:1538	a novel and soothing drug	1514:1538	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	3	33	theme	average	549:555	arg1	mass					567:570	an average molecular mass	546:570	an average molecular mass of 23.35 kDa	546:583	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	9	34	from	100 μg/mL	1416:1424	arg1	control					1405:1411	the control	1401:1411	the control at 100 μg/mL	1401:1424	However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL.					
31071403	6	35	theme	%	970:970	arg1	DS					972:973	26% DS	968:973	26% DS	968:973	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	7	36	theme	thrombin	1115:1122	arg1	time					1124:1127	thrombin time	1115:1127	thrombin time (TT)	1115:1132	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	7	36	theme	thrombin	1115:1122	arg1	TT					1130:1131	TT	1130:1131	TT	1130:1131	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	6	37	theme	Chondroitinase	891:904	arg1	AC					906:907	Chondroitinase AC	891:907	Chondroitinase AC	891:907	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	3	38	theme	molecular	557:565	arg1	mass					567:570	an average molecular mass	546:570	an average molecular mass of 23.35 kDa	546:583	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	2	39	theme	agarose-gel	370:380	arg1	electrophoresis					382:396	agarose-gel electrophoresis	370:396	agarose-gel electrophoresis	370:396	Infrared spectrum and agarose-gel electrophoresis for extracted CS/DS were also investigated.					
31071403	7	40	theme	strong	1037:1042	arg1	effect					1058:1063	a strong anticoagulant effect	1035:1063	a strong anticoagulant effect	1035:1063	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	10	41	theme	promising	1458:1466	arg1	potential					1482:1490	a promising anticoagulant potential	1456:1490	a promising anticoagulant potential	1456:1490	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	10	41	theme	promising	1458:1466	arg1	drug					1535:1538	a novel and soothing drug	1514:1538	a novel and soothing drug	1514:1538	The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.					
31071403	6	42	theme	26	968:969	arg1	%					970:970	%	970:970	%	970:970	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	8	43	theme	negative	1287:1294	arg1	control					1296:1302	the negative control	1283:1302	the negative control at a concentration of 1000 μg/mL	1283:1335	In fact, CBG prolonged significantly (p < 0.05), aPTT and PT about 2.62 and 1.26 fold, respectively, greater than that of the negative control at a concentration of 1000 μg/mL.					
31071403	4	44	theme	different	783:791	arg1	percentages					793:803	different percentages	783:803	different percentages	783:803	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	3	45	theme	purified	470:477	arg1	CS/DS					479:483	the purified CS/DS	466:483	the purified CS/DS obtained at a yield of 10%	466:510	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	0	46	theme	anticoagulant	42:54	arg1	capacity					56:63	anticoagulant capacity	42:63	anticoagulant capacity	42:63	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	3	47	contain	contains	512:519	arg2	sulfate					534:540	about 31.28% sulfate	521:540	about 31.28% sulfate	521:540	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	3	47	contain	contains	512:519	arg1	CS/DS					479:483	the purified CS/DS	466:483	the purified CS/DS obtained at a yield of 10%	466:510	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	3	47	contain	contains	512:519	arg2	mass					567:570	an average molecular mass	546:570	an average molecular mass of 23.35 kDa	546:583	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	8	48	dep	prolonged	1174:1182	arg1	p < 0.05					1199:1206	p < 0.05	1199:1206	p < 0.05	1199:1206	In fact, CBG prolonged significantly (p < 0.05), aPTT and PT about 2.62 and 1.26 fold, respectively, greater than that of the negative control at a concentration of 1000 μg/mL.					
31071403	4	49	from	disaccharides	766:778	arg1	percentages					793:803	different percentages	783:803	different percentages	783:803	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	5	50	theme	residues	863:870	arg1	ratio					841:845	the ratio	837:845	the ratio of 4:6 sulfated residues	837:870	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	5	50	theme	residues	863:870	arg1	equal					876:880	equal	876:880	equal	876:880	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	1	51	dep	corb	248:251	arg1	bone					240:243	bone	240:243	bone	240:243	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	1	51	dep	corb	248:251	arg1	the					236:238	the	236:238	the	236:238	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	3	52	theme	23.35 kDa	575:583	arg1	mass					567:570	an average molecular mass	546:570	an average molecular mass of 23.35 kDa	546:583	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	3	52	theme	23.35 kDa	575:583	arg1	sulfate					534:540	about 31.28% sulfate	521:540	about 31.28% sulfate	521:540	The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa.					
31071403	4	53	theme	disulfated	755:764	arg1	disaccharides					766:778	disulfated disaccharides	755:778	disulfated disaccharides	755:778	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	54	dep	N-acetylgalactosamine	698:718	arg1	%					724:724	8.6%	721:724	8.6%	721:724	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	54	dep	N-acetylgalactosamine	698:718	arg1	%					734:734	40.0%	730:734	40.0%	730:734	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	0	55	from	bone	110:113	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	0	55	from	bone	110:113	arg1	analysis					28:35	compositional analysis	14:35	compositional analysis	14:35	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	0	55	from	bone	110:113	arg1	capacity					56:63	anticoagulant capacity	42:63	anticoagulant capacity	42:63	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	4	56	dep	positions	673:681	arg1	4					689:689	4	689:689	4	689:689	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	56	dep	positions	673:681	arg1	N-acetylgalactosamine					698:718	the N-acetylgalactosamine	694:718	the N-acetylgalactosamine (8.6% and 40.0%, respectively)	694:749	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	4	56	dep	positions	673:681	arg1	6					683:683	6	683:683	6	683:683	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	8	57	theme	1000 μg/mL	1326:1335	arg1	concentration					1309:1321	a concentration	1307:1321	a concentration of 1000 μg/mL	1307:1335	In fact, CBG prolonged significantly (p < 0.05), aPTT and PT about 2.62 and 1.26 fold, respectively, greater than that of the negative control at a concentration of 1000 μg/mL.					
31071403	4	58	theme	monosulfated	643:654	arg1	disaccharides					656:668	monosulfated disaccharides	643:668	monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages	643:803	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	1	59	theme	first	220:224	arg1	time					226:229	the first time	216:229	the first time	216:229	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	0	60	theme	chondroitin	68:78	arg1	sulfate					97:103	chondroitin sulfate/dermatan sulfate	68:103	chondroitin sulfate/dermatan sulfate	68:103	Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).					
31071403	5	61	theme	charge	810:815	arg1	1.4					829:831	1.4	829:831	1.4	829:831	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	5	61	theme	charge	810:815	arg1	density					817:823	The charge density	806:823	The charge density	806:823	The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64.					
31071403	9	62	theme	TT	1347:1348	arg1	assay					1350:1354	TT assay	1347:1354	TT assay of CBG	1347:1361	However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL.					
31071403	7	63	theme	prothrombin	1138:1148	arg1	PT					1156:1157	PT	1156:1157	PT	1156:1157	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	7	63	theme	prothrombin	1138:1148	arg1	time					1150:1153	prothrombin time	1138:1153	prothrombin time (PT)	1138:1158	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	6	64	theme	%	959:959	arg1	CS					961:962	74% CS	957:962	74% CS	957:962	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
31071403	7	65	theme	partial	1083:1089	arg1	aPTT					1108:1111	aPTT	1108:1111	aPTT	1108:1111	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	7	65	theme	partial	1083:1089	arg1	time					1102:1105	activated partial thrombosis time	1073:1105	activated partial thrombosis time (aPTT)	1073:1112	Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT).					
31071403	1	66	theme	Chondroitin	140:150	arg1	CS/DS					178:182	CS/DS	178:182	CS/DS	178:182	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	1	66	theme	Chondroitin	140:150	arg1	sulfate					169:175	Chondroitin sulfate/dermatan sulfate	140:175	Chondroitin sulfate/dermatan sulfate (CS/DS)	140:183	Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed.					
31071403	4	67	from	positions	673:681	arg1	percentages					793:803	different percentages	783:803	different percentages	783:803	Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages.					
31071403	6	68	theme	74	957:958	arg1	%					959:959	%	959:959	%	959:959	Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS.					
30404213	0	0	theme	Streptococcus	92:104	arg1	thermophilus					106:117	Streptococcus thermophilus CH9	92:121	Streptococcus thermophilus CH9	92:121	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	5	1	theme	%	708:708	arg1	ratio					694:698	a ratio	692:698	a ratio of 69.82% and 57.09%, respectively	692:733	The EPS-1a and EPS-2a were mainly composed of mannose, in a ratio of 69.82% and 57.09%, respectively, while EPS-3a was mainly composed of glucose (63.93%), without mannose.					
30404213	7	2	theme	human	1041:1045	arg1	cells					1066:1070	human liver cancer HepG2 cells	1041:1070	human liver cancer HepG2 cells	1041:1070	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	9	3	contain	had	1259:1261	arg2	changes					1285:1291	typical morphological changes	1263:1291	typical morphological changes	1263:1291	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	9	3	contain	had	1259:1261	arg1	cells					1253:1257	HepG2 cells	1247:1257	HepG2 cells	1247:1257	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	3	4	theme	EPS-3a	497:502	arg1	weight					467:472	The average molecular weight	445:472	The average molecular weight	445:472	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	3	4	theme	EPS-3a	497:502	arg1	1.80					510:513	1.80	510:513	1.80	510:513	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	6	5	theme	sugar	903:907	arg1	chain					909:913	the sugar chain	899:913	the sugar chain of EPS-3a	899:923	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	7	6	theme	liver	1047:1051	arg1	cells					1066:1070	human liver cancer HepG2 cells	1041:1070	human liver cancer HepG2 cells	1041:1070	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	1	7	from	structure	162:170	arg1	thermophilus					258:269	Streptococcus thermophilus CH9	244:273	Streptococcus thermophilus CH9	244:273	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	2	8	theme	EPS-3a	348:353	arg1	fractions					315:323	three purified fractions	300:323	three purified fractions of EPS-1a, EPS-2a, and EPS-3a	300:353	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	8	9	from	activity	1096:1103	arg1	cells					1124:1128	HepG2 cells	1118:1128	HepG2 cells	1118:1128	The antitumor activity of EPS-3a in HepG2 cells was associated with cell apoptosis.					
30404213	10	10	theme	cytometry	1299:1307	arg1	analysis					1309:1316	Flow cytometry analysis	1294:1316	Flow cytometry analysis	1294:1316	Flow cytometry analysis showed that the cell cycle was arrested at G0/G1 phase.					
30404213	3	11	dep	1.80	510:513	arg1	10⁶					544:546	× 10⁶	542:546	× 10⁶	542:546	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	2	12	theme	EPS-1a	328:333	arg1	fractions					315:323	three purified fractions	300:323	three purified fractions of EPS-1a, EPS-2a, and EPS-3a	300:353	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	10	13	theme	Flow	1294:1297	arg1	analysis					1309:1316	Flow cytometry analysis	1294:1316	Flow cytometry analysis	1294:1316	Flow cytometry analysis showed that the cell cycle was arrested at G0/G1 phase.					
30404213	8	14	theme	antitumor	1086:1094	arg1	activity					1096:1103	The antitumor activity	1082:1103	The antitumor activity of EPS-3a in HepG2 cells	1082:1128	The antitumor activity of EPS-3a in HepG2 cells was associated with cell apoptosis.					
30404213	7	15	theme	higher	1007:1012	arg1	activity					1024:1031	higher antitumor activity	1007:1031	higher antitumor activity	1007:1031	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	3	16	theme	×	515:515	arg1	10⁶					517:519	10⁶	517:519	10⁶	517:519	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	1	17	theme	in	176:177	arg1	activity					195:202	in vitro antitumor activity	176:202	in vitro antitumor activity	176:202	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	7	18	theme	antitumor	1014:1022	arg1	activity					1024:1031	higher antitumor activity	1007:1031	higher antitumor activity	1007:1031	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	10	19	theme	cell	1334:1337	arg1	cycle					1339:1343	the cell cycle	1330:1343	the cell cycle	1330:1343	Flow cytometry analysis showed that the cell cycle was arrested at G0/G1 phase.					
30404213	6	20	theme	EPS-3a	918:923	arg1	chain					909:913	the sugar chain	899:913	the sugar chain of EPS-3a	899:923	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	4	21	from	others	626:631	arg1	different					607:615	different	607:615	different	607:615	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	4	21	from	others	626:631	arg1	composition					568:578	The monosaccharide composition	549:578	The monosaccharide composition of EPS-3a	549:588	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	2	22	theme	DEAE-52	393:399	arg1	cellulose					401:409	DEAE-52 cellulose	393:409	DEAE-52 cellulose	393:409	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	9	23	theme	EPS-3a	1239:1244	arg1	treatment					1226:1234	the treatment	1222:1234	the treatment of EPS-3a	1222:1244	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	1	24	dep	structure	162:170	arg1	the					146:148	the	146:148	the	146:148	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	25	theme	antitumor	185:193	arg1	activity					195:202	in vitro antitumor activity	176:202	in vitro antitumor activity	176:202	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	6	26	theme	protein	878:884	arg1	particles					886:894	protein particles	878:894	protein particles	878:894	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	8	27	theme	cell	1150:1153	arg1	apoptosis					1155:1163	cell apoptosis	1150:1163	cell apoptosis	1150:1163	The antitumor activity of EPS-3a in HepG2 cells was associated with cell apoptosis.					
30404213	10	28	theme	G0/G1	1361:1365	arg1	phase					1367:1371	G0/G1 phase	1361:1371	G0/G1 phase	1361:1371	Flow cytometry analysis showed that the cell cycle was arrested at G0/G1 phase.					
30404213	6	29	theme	protein-containing	942:959	arg1	EPS-3a					929:934	EPS-3a	929:934	EPS-3a	929:934	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	6	29	theme	protein-containing	942:959	arg1	polysaccharide					961:974	a protein-containing polysaccharide	940:974	a protein-containing polysaccharide	940:974	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	0	30	theme	Preliminary	14:24	arg1	Structure					26:34	Preliminary Structure	14:34	Preliminary Structure	14:34	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	9	31	theme	HepG2	1247:1251	arg1	cells					1253:1257	HepG2 cells	1247:1257	HepG2 cells	1247:1257	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	2	32	theme	purified	306:313	arg1	fractions					315:323	three purified fractions	300:323	three purified fractions of EPS-1a, EPS-2a, and EPS-3a	300:353	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	3	33	theme	EPS-1a	477:482	arg1	weight					467:472	The average molecular weight	445:472	The average molecular weight	445:472	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	3	33	theme	EPS-1a	477:482	arg1	1.80					510:513	1.80	510:513	1.80	510:513	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	0	34	theme	Antitumor	40:48	arg1	Activity					50:57	Antitumor Activity	40:57	Antitumor Activity	40:57	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	1	35	from	thermophilus	258:269	arg1	exopolysaccharides					213:230	three exopolysaccharides	207:230	three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9	207:273	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	35	from	thermophilus	258:269	arg1	EPSs					233:236	EPSs	233:236	EPSs	233:236	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	35	from	thermophilus	258:269	arg1	activity					195:202	in vitro antitumor activity	176:202	in vitro antitumor activity	176:202	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	35	from	thermophilus	258:269	arg1	structure					162:170	preliminary structure	150:170	preliminary structure	150:170	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	36	theme	exopolysaccharides	213:230	arg1	activity					195:202	in vitro antitumor activity	176:202	in vitro antitumor activity	176:202	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	36	theme	exopolysaccharides	213:230	arg1	structure					162:170	preliminary structure	150:170	preliminary structure	150:170	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	2	37	theme	Sephadex	415:422	arg1	G-100					424:428	Sephadex G-100	415:428	Sephadex G-100	415:428	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	5	38	theme	%	719:719	arg1	ratio					694:698	a ratio	692:698	a ratio of 69.82% and 57.09%, respectively	692:733	The EPS-1a and EPS-2a were mainly composed of mannose, in a ratio of 69.82% and 57.09%, respectively, while EPS-3a was mainly composed of glucose (63.93%), without mannose.					
30404213	8	39	theme	HepG2	1118:1122	arg1	cells					1124:1128	HepG2 cells	1118:1128	HepG2 cells	1118:1128	The antitumor activity of EPS-3a in HepG2 cells was associated with cell apoptosis.					
30404213	7	40	theme	cancer	1053:1058	arg1	cells					1066:1070	human liver cancer HepG2 cells	1041:1070	human liver cancer HepG2 cells	1041:1070	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	9	41	theme	typical	1263:1269	arg1	changes					1285:1291	typical morphological changes	1263:1291	typical morphological changes	1263:1291	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	1	42	dep	in	176:177	arg1	vitro					179:183	vitro	179:183	vitro	179:183	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	7	43	theme	HepG2	1060:1064	arg1	cells					1066:1070	human liver cancer HepG2 cells	1041:1070	human liver cancer HepG2 cells	1041:1070	Furthermore, EPS-3a exhibited higher antitumor activity against human liver cancer HepG2 cells in vitro.					
30404213	9	44	theme	morphological	1271:1283	arg1	changes					1285:1291	typical morphological changes	1263:1291	typical morphological changes	1263:1291	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	9	45	theme	HE	1166:1167	arg1	staining					1169:1176	HE staining	1166:1176	HE staining	1166:1176	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	1	46	theme	present	131:137	arg1	study					139:143	the present study	127:143	the present study	127:143	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	6	47	theme	surface	824:830	arg1	morphology					832:841	the surface morphology	820:841	the surface morphology observed	820:850	In addition, the surface morphology observed suggested that there were protein particles on the sugar chain of EPS-3a and EPS-3a was a protein-containing polysaccharide.					
30404213	2	48	theme	EPS-2a	336:341	arg1	fractions					315:323	three purified fractions	300:323	three purified fractions of EPS-1a, EPS-2a, and EPS-3a	300:353	Then, three purified fractions of EPS-1a, EPS-2a, and EPS-3a were obtained by chromatography using DEAE-52 cellulose and Sephadex G-100, respectively.					
30404213	3	49	theme	EPS-2a	485:490	arg1	weight					467:472	The average molecular weight	445:472	The average molecular weight	445:472	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	3	49	theme	EPS-2a	485:490	arg1	1.80					510:513	1.80	510:513	1.80	510:513	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	4	50	theme	monosaccharide	553:566	arg1	different					607:615	different	607:615	different	607:615	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	4	50	theme	monosaccharide	553:566	arg1	composition					568:578	The monosaccharide composition	549:578	The monosaccharide composition of EPS-3a	549:588	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	9	51	theme	Hoechst	1182:1188	arg1	staining					1196:1203	Hoechst 33342 staining	1182:1203	Hoechst 33342 staining	1182:1203	HE staining and Hoechst 33342 staining showed that with the treatment of EPS-3a, HepG2 cells had typical morphological changes.					
30404213	3	52	theme	molecular	457:465	arg1	weight					467:472	The average molecular weight	445:472	The average molecular weight	445:472	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	3	52	theme	molecular	457:465	arg1	1.80					510:513	1.80	510:513	1.80	510:513	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	1	53	from	activity	195:202	arg1	thermophilus					258:269	Streptococcus thermophilus CH9	244:273	Streptococcus thermophilus CH9	244:273	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	3	54	theme	average	449:455	arg1	weight					467:472	The average molecular weight	445:472	The average molecular weight	445:472	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	3	54	theme	average	449:455	arg1	1.80					510:513	1.80	510:513	1.80	510:513	The average molecular weight of EPS-1a, EPS-2a, and EPS-3a, were 1.80 × 10⁶, 1.06 × 10⁶ and 1.05 × 10⁶.					
30404213	4	55	theme	EPS-3a	583:588	arg1	different					607:615	different	607:615	different	607:615	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	4	55	theme	EPS-3a	583:588	arg1	composition					568:578	The monosaccharide composition	549:578	The monosaccharide composition of EPS-3a	549:588	The monosaccharide composition of EPS-3a was dramatically different from the others.					
30404213	0	56	theme	Exopolysaccharide	62:78	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	0	56	theme	Exopolysaccharide	62:78	arg1	Structure					26:34	Preliminary Structure	14:34	Preliminary Structure	14:34	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	0	56	theme	Exopolysaccharide	62:78	arg1	Activity					50:57	Antitumor Activity	40:57	Antitumor Activity	40:57	Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced by Streptococcus thermophilus CH9.					
30404213	8	57	theme	EPS-3a	1108:1113	arg1	activity					1096:1103	The antitumor activity	1082:1103	The antitumor activity of EPS-3a in HepG2 cells	1082:1128	The antitumor activity of EPS-3a in HepG2 cells was associated with cell apoptosis.					
30404213	1	58	theme	preliminary	150:160	arg1	structure					162:170	preliminary structure	150:170	preliminary structure	150:170	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
30404213	1	59	theme	Streptococcus	244:256	arg1	thermophilus					258:269	Streptococcus thermophilus CH9	244:273	Streptococcus thermophilus CH9	244:273	In the present study, the preliminary structure and in vitro antitumor activity of three exopolysaccharides (EPSs) from Streptococcus thermophilus CH9 were investigated.					
32450036	0	0	theme	Fruiting	104:111	arg1	Bodies					113:118	Fruiting Bodies	104:118	Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	104:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	8	1	theme	weight	1432:1437	arg1	distribution					1439:1450	molecular weight distribution	1422:1450	molecular weight distribution	1422:1450	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	2	2	theme	water-soluble	350:362	arg1	NDPs					395:398	NDPs	395:398	NDPs	395:398	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	2	theme	water-soluble	350:362	arg1	polysaccharides					378:392	water-soluble nondigestible polysaccharides	350:392	water-soluble nondigestible polysaccharides (NDPs)	350:399	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	3	theme	physicochemical	476:490	arg1	properties					492:501	their physicochemical properties	470:501	their physicochemical properties	470:501	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	8	4	from	differences	1495:1505	arg1	activity					1528:1535	anti-inflammatory activity	1510:1535	anti-inflammatory activity	1510:1535	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	3	5	theme	%	656:656	arg1	CP					638:639	CP	638:639	CP	638:639	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	3	5	theme	%	656:656	arg1	NDP					645:647	NDP	645:647	NDP	645:647	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	7	6	theme	bioassay	1140:1147	arg1	results					1149:1155	The bioassay results	1136:1155	The bioassay results	1136:1155	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	0	7	from	Isolation	0:8	arg1	Bodies					113:118	Fruiting Bodies	104:118	Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	104:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	7	8	theme	TNF-α	1295:1299	arg1	production					1301:1310	nitric oxide, IL-1β, and TNF-α production	1270:1310	nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages	1270:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	4	9	theme	predominant	752:762	arg1	mannose					783:789	mannose	783:789	mannose	783:789	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	4	9	theme	predominant	752:762	arg1	monosaccharide					764:777	the predominant monosaccharide	748:777	the predominant monosaccharide	748:777	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	6	10	theme	helix	1116:1120	arg1	conformation					1122:1133	a triple helix conformation	1107:1133	a triple helix conformation	1107:1133	Furthermore, CP but not NDP had a triple helix conformation.					
32450036	6	11	contain	had	1103:1105	arg2	conformation					1122:1133	a triple helix conformation	1107:1133	a triple helix conformation	1107:1133	Furthermore, CP but not NDP had a triple helix conformation.					
32450036	6	11	contain	had	1103:1105	arg1	NDP					1099:1101	NDP	1099:1101	CP but not NDP	1088:1101	Furthermore, CP but not NDP had a triple helix conformation.					
32450036	6	11	contain	had	1103:1105	arg1	CP					1088:1089	CP	1088:1089	CP but not NDP	1088:1101	Furthermore, CP but not NDP had a triple helix conformation.					
32450036	5	12	dep	major	923:927	arg1	kDa					934:936	3.1 kDa	930:936	3.1 kDa	930:936	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	12	dep	major	923:927	arg1	kDa					947:949	21.5 kDa	942:949	21.5 kDa	942:949	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	12	dep	major	923:927	arg1	populations					993:1003	macromolecular populations	978:1003	macromolecular populations	978:1003	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	4	13	theme	similar	703:709	arg1	composition					726:736	a similar monosaccharide composition	701:736	a similar monosaccharide composition	701:736	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	0	14	theme	Cordyceps	134:142	arg1	Ascomycetes					153:163	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	15	from	Properties	59:68	arg1	Bodies					113:118	Fruiting Bodies	104:118	Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	104:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	2	16	theme	-stimulated	561:571	arg1	macrophages					582:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	6	17	theme	triple	1109:1114	arg1	conformation					1122:1133	a triple helix conformation	1107:1133	a triple helix conformation	1107:1133	Furthermore, CP but not NDP had a triple helix conformation.					
32450036	7	18	theme	nitric	1270:1275	arg1	oxide					1277:1281	nitric oxide	1270:1281	nitric oxide	1270:1281	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	0	19	theme	Cultivated	123:132	arg1	Ascomycetes					153:163	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	7	20	contain	had	1235:1237	arg1	NDP					1231:1233	NDP	1231:1233	NDP	1231:1233	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	7	20	contain	had	1235:1237	arg2	effects					1259:1265	stronger inhibitory effects	1239:1265	stronger inhibitory effects	1239:1265	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	5	21	contain	contained	1018:1026	arg1	NDP					1014:1016	NDP	1014:1016	NDP	1014:1016	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	21	contain	contained	1018:1026	arg2	population					1052:1061	only one macromolecular population	1028:1061	only one macromolecular population (24.4 kDa)	1028:1072	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	21	contain	contained	1018:1026	arg2	kDa					1069:1071	24.4 kDa	1064:1071	24.4 kDa	1064:1071	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	1	22	theme	medicinal	190:198	arg1	fungus					200:205	a medicinal fungus	188:205	a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases	188:301	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	1	22	theme	medicinal	190:198	arg1	cicadae					177:183	Cordyceps cicadae	167:183	Cordyceps cicadae	167:183	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	2	23	theme	C.	454:455	arg1	cicadae					457:463	cultivated C. cicadae	443:463	cultivated C. cicadae	443:463	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	24	theme	anti-inflammatory	507:523	arg1	activity					525:532	anti-inflammatory activity	507:532	anti-inflammatory activity	507:532	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	25	theme	crude	319:323	arg1	CP					342:343	CP	342:343	CP	342:343	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	25	theme	crude	319:323	arg1	polysaccharides					325:339	crude polysaccharides	319:339	crude polysaccharides (CP)	319:344	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	7	26	contain	possess	1195:1201	arg2	activity					1221:1228	anti-inflammatory activity	1203:1228	anti-inflammatory activity	1203:1228	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	7	26	contain	possess	1195:1201	arg1	NDP					1191:1193	NDP	1191:1193	NDP	1191:1193	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	7	26	contain	possess	1195:1201	arg1	CP					1184:1185	CP	1184:1185	CP	1184:1185	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	2	27	theme	cultivated	443:452	arg1	cicadae					457:463	cultivated C. cicadae	443:463	cultivated C. cicadae	443:463	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	1	28	theme	kidney	287:292	arg1	diseases					294:301	kidney diseases	287:301	kidney diseases	287:301	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	3	29	theme	%	666:666	arg1	CP					638:639	CP	638:639	CP	638:639	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	3	29	theme	%	666:666	arg1	NDP					645:647	NDP	645:647	NDP	645:647	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	4	30	theme	composition	726:736	arg1	mannose					783:789	mannose	783:789	mannose	783:789	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	4	30	theme	composition	726:736	arg1	monosaccharide					764:777	the predominant monosaccharide	748:777	the predominant monosaccharide	748:777	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	2	31	theme	RAW264.7	573:580	arg1	macrophages					582:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	7	32	theme	RAW264.7	1330:1337	arg1	macrophages					1339:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	7	33	from	production	1301:1310	arg1	macrophages					1339:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	2	34	theme	fruiting	424:431	arg1	bodies					433:438	the fruiting bodies	420:438	the fruiting bodies of cultivated C. cicadae	420:463	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	0	35	theme	Anti-Inflammatory	11:27	arg1	Activity					29:36	Anti-Inflammatory Activity	11:36	Anti-Inflammatory Activity	11:36	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	8	36	from	differences	1390:1400	arg1	conformation					1405:1416	conformation	1405:1416	conformation	1405:1416	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	8	36	from	differences	1390:1400	arg1	distribution					1439:1450	molecular weight distribution	1422:1450	molecular weight distribution	1422:1450	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	0	37	theme	cicadae	144:150	arg1	Ascomycetes					153:163	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Cultivated Cordyceps cicadae (Ascomycetes)	123:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	2	38	from	properties	492:501	arg1	macrophages					582:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	7	39	theme	inhibitory	1248:1257	arg1	effects					1259:1265	stronger inhibitory effects	1239:1265	stronger inhibitory effects	1239:1265	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	0	40	theme	Physico-chemical	42:57	arg1	Properties					59:68	Physico-chemical Properties	42:68	Physico-chemical Properties	42:68	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	5	41	theme	molecular	842:850	arg1	distribution					859:870	molecular weight distribution	842:870	molecular weight distribution	842:870	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	1	42	theme	traditional	225:235	arg1	medicine					245:252	traditional Chinese medicine	225:252	traditional Chinese medicine	225:252	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	0	43	theme	Ascomycetes	153:163	arg1	Bodies					113:118	Fruiting Bodies	104:118	Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	104:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	5	44	theme	macromolecular	1037:1050	arg1	kDa					1069:1071	24.4 kDa	1064:1071	24.4 kDa	1064:1071	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	44	theme	macromolecular	1037:1050	arg1	population					1052:1061	only one macromolecular population	1028:1061	only one macromolecular population (24.4 kDa)	1028:1072	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	7	45	theme	LPS-stimulated	1315:1328	arg1	macrophages					1339:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	LPS-stimulated RAW264.7 macrophages	1315:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	5	46	theme	weight	852:857	arg1	distribution					859:870	molecular weight distribution	842:870	molecular weight distribution	842:870	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	1	47	theme	Chinese	237:243	arg1	medicine					245:252	traditional Chinese medicine	225:252	traditional Chinese medicine	225:252	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	8	48	theme	anti-inflammatory	1510:1526	arg1	activity					1528:1535	anti-inflammatory activity	1510:1535	anti-inflammatory activity	1510:1535	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	5	49	theme	macromolecular	978:991	arg1	populations					993:1003	macromolecular populations	978:1003	macromolecular populations	978:1003	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	2	50	theme	cicadae	457:463	arg1	bodies					433:438	the fruiting bodies	420:438	the fruiting bodies of cultivated C. cicadae	420:463	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	5	51	contain	possessed	909:917	arg2	major					923:927	major	923:927	major	923:927	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	51	contain	possessed	909:917	arg2	minor					960:964	minor	960:964	minor	960:964	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	51	contain	possessed	909:917	arg1	CP					906:907	CP	906:907	CP	906:907	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	5	51	contain	possessed	909:917	arg2	kDa					973:975	678.2 kDa	967:975	678.2 kDa	967:975	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	7	52	theme	IL-1β	1284:1288	arg1	production					1301:1310	nitric oxide, IL-1β, and TNF-α production	1270:1310	nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages	1270:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	2	53	from	activity	525:532	arg1	macrophages					582:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	537:592	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	4	54	theme	monosaccharide	711:724	arg1	composition					726:736	a similar monosaccharide composition	701:736	a similar monosaccharide composition	701:736	CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose.					
32450036	5	55	from	Differences	827:837	arg1	distribution					859:870	molecular weight distribution	842:870	molecular weight distribution	842:870	Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa).					
32450036	7	56	theme	anti-inflammatory	1203:1219	arg1	activity					1221:1228	anti-inflammatory activity	1203:1228	anti-inflammatory activity	1203:1228	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	8	57	theme	molecular	1422:1430	arg1	distribution					1439:1450	molecular weight distribution	1422:1450	molecular weight distribution	1422:1450	From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.					
32450036	3	58	theme	NDP	645:647	arg1	yields					628:633	yields	628:633	yields of CP and NDP of 3.42% and 1.17%, respectively	628:680	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	2	59	theme	nondigestible	364:376	arg1	NDPs					395:398	NDPs	395:398	NDPs	395:398	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	2	59	theme	nondigestible	364:376	arg1	polysaccharides					378:392	water-soluble nondigestible polysaccharides	350:392	water-soluble nondigestible polysaccharides (NDPs)	350:399	In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined.					
32450036	0	60	theme	Polysaccharides	83:97	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	60	theme	Polysaccharides	83:97	arg1	Activity					29:36	Anti-Inflammatory Activity	11:36	Anti-Inflammatory Activity	11:36	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	60	theme	Polysaccharides	83:97	arg1	Properties					59:68	Physico-chemical Properties	42:68	Physico-chemical Properties	42:68	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	61	from	Bodies	113:118	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	61	from	Bodies	113:118	arg1	Activity					29:36	Anti-Inflammatory Activity	11:36	Anti-Inflammatory Activity	11:36	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	61	from	Bodies	113:118	arg1	Properties					59:68	Physico-chemical Properties	42:68	Physico-chemical Properties	42:68	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	61	from	Bodies	113:118	arg1	Polysaccharides					83:97	Bioactive Polysaccharides	73:97	Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	73:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	7	62	theme	stronger	1239:1246	arg1	effects					1259:1265	stronger inhibitory effects	1239:1265	stronger inhibitory effects	1239:1265	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	7	63	theme	oxide	1277:1281	arg1	production					1301:1310	nitric oxide, IL-1β, and TNF-α production	1270:1310	nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages	1270:1349	The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages.					
32450036	0	64	theme	Bioactive	73:81	arg1	Polysaccharides					83:97	Bioactive Polysaccharides	73:97	Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	73:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	0	65	from	Activity	29:36	arg1	Bodies					113:118	Fruiting Bodies	104:118	Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)	104:164	Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes).					
32450036	3	66	theme	CP	638:639	arg1	yields					628:633	yields	628:633	yields of CP and NDP of 3.42% and 1.17%, respectively	628:680	The results showed yields of CP and NDP of 3.42% and 1.17%, respectively.					
32450036	1	67	theme	Cordyceps	167:175	arg1	fungus					200:205	a medicinal fungus	188:205	a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases	188:301	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
32450036	1	67	theme	Cordyceps	167:175	arg1	cicadae					177:183	Cordyceps cicadae	167:183	Cordyceps cicadae	167:183	Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases.					
31472848	0	0	theme	TLR/NF-κB	99:107	arg1	pathway					116:122	TLR/NF-κB signal pathway	99:122	TLR/NF-κB signal pathway	99:122	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	9	1	theme	MyD88	1286:1290	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	1	theme	MyD88	1286:1290	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	5	2	theme	fucoidan	691:698	arg1	properties					677:686	anti-inflammatory properties	659:686	anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF)	659:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	1	3	theme	compounds	175:183	arg1	seaweeds					131:138	Brown seaweeds	125:138	Brown seaweeds	125:138	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	1	3	theme	compounds	175:183	arg1	source					155:160	well-known source	144:160	well-known source	144:160	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	4	4	theme	seaweeds	498:505	arg1	applications					482:493	applications	482:493	applications of seaweeds	482:505	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	4	5	theme	bioactive	615:623	arg1	properties					625:634	their bioactive properties	609:634	their bioactive properties	609:634	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	10	6	theme	inflammatory	1456:1467	arg1	responses					1469:1477	LPS-induced inflammatory responses	1444:1477	LPS-induced inflammatory responses	1444:1477	The results, obtained from this study demonstrated PCF has a potential to inhibit LPS-induced inflammatory responses via blocking TLR/MyD88/ NF-κB signal transduction.					
31472848	6	7	theme	mono	883:886	arg1	composition					894:904	mono sugar composition	883:904	mono sugar composition	883:904	PCF was characterized using NMR, FT-IR, and HPAE-PAD spectrum (for mono sugar composition).					
31472848	2	8	from	centuries	343:351	arg1	countries					362:370	Asian countries	356:370	Asian countries	356:370	Traditionally, seaweeds used as ingredients in medicine for many centuries in Asian countries.					
31472848	2	9	theme	Asian	356:360	arg1	countries					362:370	Asian countries	356:370	Asian countries	356:370	Traditionally, seaweeds used as ingredients in medicine for many centuries in Asian countries.					
31472848	4	10	theme	poor	530:533	arg1	understanding					535:547	poor understanding	530:547	poor understanding of their structural features and mechanisms responsible for their bioactive properties	530:634	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	10	11	theme	LPS-induced	1444:1454	arg1	responses					1469:1477	LPS-induced inflammatory responses	1444:1477	LPS-induced inflammatory responses	1444:1477	The results, obtained from this study demonstrated PCF has a potential to inhibit LPS-induced inflammatory responses via blocking TLR/MyD88/ NF-κB signal transduction.					
31472848	0	12	attach	isolated	9:16	arg2	Fucoidan					0:7	Fucoidan	0:7	Fucoidan isolated from Padina commersonii	0:40	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	0	12	attach	isolated	9:16	arg1	commersonii					30:40	Padina commersonii	23:40	Padina commersonii	23:40	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	7	13	from	rich	936:939	arg1	sulfate					955:961	sulfate	955:961	sulfate	955:961	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	7	13	from	rich	936:939	arg1	structure					984:992	a similar structure	974:992	a similar structure to the commercial fucoidan	974:1019	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	7	13	from	rich	936:939	arg1	fucose					944:949	fucose	944:949	fucose	944:949	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	0	14	theme	signal	109:114	arg1	pathway					116:122	TLR/NF-κB signal pathway	99:122	TLR/NF-κB signal pathway	99:122	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	0	15	from	inflammation	62:73	arg1	macrophages					78:88	macrophages	78:88	macrophages blocking TLR/NF-κB signal pathway	78:122	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	8	16	theme	RT-qPCR	1040:1046	arg1	analysis					1048:1055	RT-qPCR analysis	1040:1055	RT-qPCR analysis	1040:1055	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	5	17	theme	present	644:650	arg1	study					652:656	the present study	640:656	the present study	640:656	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	18	theme	LPS-activated	779:791	arg1	macrophages					803:813	LPS-activated RAW 264.7 macrophages	779:813	LPS-activated RAW 264.7 macrophages	779:813	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	10	19	theme	NF-κB	1503:1507	arg1	transduction					1516:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	The results, obtained from this study demonstrated PCF has a potential to inhibit LPS-induced inflammatory responses via blocking TLR/MyD88/ NF-κB signal transduction.					
31472848	9	20	theme	transcriptional	1337:1351	arg1	factors					1353:1359	NF-κB transcriptional factors	1331:1359	NF-κB transcriptional factors	1331:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	5	21	theme	RAW	793:795	arg1	macrophages					803:813	LPS-activated RAW 264.7 macrophages	779:813	LPS-activated RAW 264.7 macrophages	779:813	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	22	theme	anti-inflammatory	659:675	arg1	properties					677:686	anti-inflammatory properties	659:686	anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF)	659:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	7	23	from	structure	984:992	arg1	rich					936:939	rich	936:939	rich	936:939	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	3	24	theme	many	411:414	arg1	metabolites					416:426	many metabolites	411:426	many metabolites found in seaweeds	411:444	However, the protective mechanisms of many metabolites found in seaweeds are remains to be determined.					
31472848	9	25	theme	NF-κB	1331:1335	arg1	factors					1353:1359	NF-κB transcriptional factors	1331:1359	NF-κB transcriptional factors	1331:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	26	theme	LPS-activated	1218:1230	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	26	theme	LPS-activated	1218:1230	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	27	theme	known	1302:1306	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	27	theme	known	1302:1306	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	8	28	theme	PCF	1106:1108	arg1	effects					1095:1101	the protective effects	1080:1101	the protective effects of PCF after LPS challenge using RAW 264.7 macrophages	1080:1156	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	3	29	theme	metabolites	416:426	arg1	mechanisms					397:406	the protective mechanisms	382:406	the protective mechanisms of many metabolites found in seaweeds	382:444	However, the protective mechanisms of many metabolites found in seaweeds are remains to be determined.					
31472848	4	30	theme	mechanisms	582:591	arg1	understanding					535:547	poor understanding	530:547	poor understanding of their structural features and mechanisms responsible for their bioactive properties	530:634	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	8	31	theme	Western	1022:1028	arg1	blots					1030:1034	Western blots	1022:1034	Western blots	1022:1034	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	2	32	theme	many	338:341	arg1	centuries					343:351	many centuries	338:351	many centuries in Asian countries	338:370	Traditionally, seaweeds used as ingredients in medicine for many centuries in Asian countries.					
31472848	0	33	theme	Padina	23:28	arg1	commersonii					30:40	Padina commersonii	23:40	Padina commersonii	23:40	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	9	34	theme	mRNA	1232:1235	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	34	theme	mRNA	1232:1235	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	8	35	theme	protective	1084:1093	arg1	effects					1095:1101	the protective effects	1080:1101	the protective effects of PCF after LPS challenge using RAW 264.7 macrophages	1080:1156	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	8	36	used	used	1062:1065	arg2	blots					1030:1034	Western blots	1022:1034	Western blots	1022:1034	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	8	36	used	used	1062:1065	arg2	analysis					1048:1055	RT-qPCR analysis	1040:1055	RT-qPCR analysis	1040:1055	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	1	37	with	metabolites	229:239	arg1	properties					266:275	promising bioactive properties	246:275	promising bioactive properties	246:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	9	38	theme	protein	1241:1247	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	38	theme	protein	1241:1247	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	1	39	theme	Brown	125:129	arg1	seaweeds					131:138	Brown seaweeds	125:138	Brown seaweeds	125:138	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	1	39	theme	Brown	125:129	arg1	source					155:160	well-known source	144:160	well-known source	144:160	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	4	40	theme	responsible	593:603	arg1	features					569:576	their structural features	552:576	their structural features	552:576	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	9	41	theme	expression	1249:1258	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	41	theme	expression	1249:1258	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	1	42	theme	secondary	219:227	arg1	metabolites					229:239	secondary metabolites	219:239	secondary metabolites with promising bioactive properties	219:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	10	43	theme	signal	1509:1514	arg1	transduction					1516:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	The results, obtained from this study demonstrated PCF has a potential to inhibit LPS-induced inflammatory responses via blocking TLR/MyD88/ NF-κB signal transduction.					
31472848	6	44	theme	sugar	888:892	arg1	composition					894:904	mono sugar composition	883:904	mono sugar composition	883:904	PCF was characterized using NMR, FT-IR, and HPAE-PAD spectrum (for mono sugar composition).					
31472848	9	45	theme	factors	1353:1359	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	45	theme	factors	1353:1359	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	1	46	theme	metabolites	229:239	arg1	variety					208:214	a variety	206:214	a variety of secondary metabolites with promising bioactive properties	206:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	1	46	theme	metabolites	229:239	arg1	metabolites					229:239	secondary metabolites	219:239	secondary metabolites with promising bioactive properties	219:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	5	47	theme	brown	718:722	arg1	PCF					752:754	PCF	752:754	PCF	752:754	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	47	theme	brown	718:722	arg1	commersonii					739:749	the brown seaweed Padina commersonii	714:749	the brown seaweed Padina commersonii (PCF)	714:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	3	48	located	found	428:432	arg2	metabolites					416:426	many metabolites	411:426	many metabolites found in seaweeds	411:444	However, the protective mechanisms of many metabolites found in seaweeds are remains to be determined.					
31472848	3	48	located	found	428:432	arg1	seaweeds					437:444	seaweeds	437:444	seaweeds	437:444	However, the protective mechanisms of many metabolites found in seaweeds are remains to be determined.					
31472848	0	49	theme	LPS-induced	50:60	arg1	inflammation					62:73	LPS-induced inflammation	50:73	LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway	50:122	Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway.					
31472848	4	50	theme	features	569:576	arg1	understanding					535:547	poor understanding	530:547	poor understanding of their structural features and mechanisms responsible for their bioactive properties	530:634	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	5	51	theme	seaweed	724:730	arg1	PCF					752:754	PCF	752:754	PCF	752:754	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	51	theme	seaweed	724:730	arg1	commersonii					739:749	the brown seaweed Padina commersonii	714:749	the brown seaweed Padina commersonii (PCF)	714:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	9	52	theme	TLR2	1270:1273	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	52	theme	TLR2	1270:1273	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	10	53	theme	TLR/MyD88/	1492:1501	arg1	transduction					1516:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	TLR/MyD88/ NF-κB signal transduction	1492:1527	The results, obtained from this study demonstrated PCF has a potential to inhibit LPS-induced inflammatory responses via blocking TLR/MyD88/ NF-κB signal transduction.					
31472848	7	54	theme	commercial	1001:1010	arg1	fucoidan					1012:1019	the commercial fucoidan	997:1019	the commercial fucoidan	997:1019	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	7	55	from	fucose	944:949	arg1	rich					936:939	rich	936:939	rich	936:939	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	5	56	attach	isolated	700:707	arg1	PCF					752:754	PCF	752:754	PCF	752:754	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	56	attach	isolated	700:707	arg1	commersonii					739:749	the brown seaweed Padina commersonii	714:749	the brown seaweed Padina commersonii (PCF)	714:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	56	attach	isolated	700:707	arg2	fucoidan					691:698	fucoidan	691:698	fucoidan isolated from the brown seaweed Padina commersonii (PCF)	691:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	4	57	theme	structural	558:567	arg1	features					569:576	their structural features	552:576	their structural features	552:576	Thus, applications of seaweeds are limited because of poor understanding of their structural features and mechanisms responsible for their bioactive properties.					
31472848	5	58	theme	Padina	732:737	arg1	PCF					752:754	PCF	752:754	PCF	752:754	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	5	58	theme	Padina	732:737	arg1	commersonii					739:749	the brown seaweed Padina commersonii	714:749	the brown seaweed Padina commersonii (PCF)	714:755	In the present study, anti-inflammatory properties of fucoidan isolated from the brown seaweed Padina commersonii (PCF) was evaluated against LPS-activated RAW 264.7 macrophages.					
31472848	1	59	theme	well-known	144:153	arg1	seaweeds					131:138	Brown seaweeds	125:138	Brown seaweeds	125:138	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	1	59	theme	well-known	144:153	arg1	source					155:160	well-known source	144:160	well-known source	144:160	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	8	60	theme	LPS	1116:1118	arg1	challenge					1120:1128	LPS challenge	1116:1128	LPS challenge using RAW 264.7 macrophages	1116:1156	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	9	61	theme	TLR4	1276:1279	arg1	levels					1260:1265	LPS-activated mRNA and protein expression levels	1218:1265	LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors	1218:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	9	61	theme	TLR4	1276:1279	arg1	inducers/activators					1308:1326	known inducers/activators	1302:1326	known inducers/activators of NF-κB transcriptional factors	1302:1359	According to the results, PCF significantly down-regulated LPS-activated mRNA and protein expression levels of TLR2, TLR4, and MyD88 which are known inducers/activators of NF-κB transcriptional factors.					
31472848	7	62	from	sulfate	955:961	arg1	rich					936:939	rich	936:939	rich	936:939	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	1	63	theme	promising	246:254	arg1	properties					266:275	promising bioactive properties	246:275	promising bioactive properties	246:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	8	64	theme	RAW	1136:1138	arg1	macrophages					1146:1156	RAW 264.7 macrophages	1136:1156	RAW 264.7 macrophages	1136:1156	Western blots and RT-qPCR analysis were used to determine the protective effects of PCF after LPS challenge using RAW 264.7 macrophages.					
31472848	6	65	theme	HPAE-PAD	860:867	arg1	spectrum					869:876	HPAE-PAD spectrum	860:876	HPAE-PAD spectrum	860:876	PCF was characterized using NMR, FT-IR, and HPAE-PAD spectrum (for mono sugar composition).					
31472848	2	66	from	ingredients	310:320	arg1	medicine					325:332	medicine	325:332	medicine	325:332	Traditionally, seaweeds used as ingredients in medicine for many centuries in Asian countries.					
31472848	1	67	theme	bioactive	256:264	arg1	properties					266:275	promising bioactive properties	246:275	promising bioactive properties	246:275	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	7	68	theme	similar	976:982	arg1	structure					984:992	a similar structure	974:992	a similar structure to the commercial fucoidan	974:1019	It was observed that PCF is rich in fucose and sulfate as well as a similar structure to the commercial fucoidan.					
31472848	1	69	theme	bioactive	165:173	arg1	compounds					175:183	bioactive compounds	165:183	bioactive compounds	165:183	Brown seaweeds are well-known source of bioactive compounds, which are producing a variety of secondary metabolites with promising bioactive properties.					
31472848	2	70	used	used	302:305	arg2	seaweeds					293:300	seaweeds	293:300	seaweeds	293:300	Traditionally, seaweeds used as ingredients in medicine for many centuries in Asian countries.					
31472848	3	71	theme	protective	386:395	arg1	mechanisms					397:406	the protective mechanisms	382:406	the protective mechanisms of many metabolites found in seaweeds	382:444	However, the protective mechanisms of many metabolites found in seaweeds are remains to be determined.					
31362136	0	0	theme	Artemisia	75:83	arg1	annua					85:89	Artemisia annua	75:89	Artemisia annua	75:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	4	1	theme	salt	465:468	arg1	stress					470:475	salt stress	465:475	salt stress	465:475	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	4	2	from	reduction	487:495	arg1	biomass					504:510	biomass	504:510	biomass	504:510	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	4	2	from	reduction	487:495	arg1	length					516:521	length	516:521	length	516:521	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	6	3	located	found	749:753	arg2	changes					736:742	The main changes	727:742	The main changes	727:742	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	3	located	found	749:753	arg1	polysaccharides					769:783	the pectic polysaccharides	758:783	the pectic polysaccharides	758:783	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	4	theme	methyl	875:880	arg1	esterification					882:895	the methyl esterification	871:895	the methyl esterification	871:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	5	5	theme	hot	628:630	arg1	water					632:636	hot water	628:636	hot water	628:636	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	9	6	theme	salt	1174:1177	arg1	stress					1179:1184	salt stress	1174:1184	salt stress	1174:1184	The results indicated adaptations in the cell wall of A. annua under salt stress.					
31362136	0	7	from	Changes	0:6	arg1	structure					31:39	structure	31:39	structure	31:39	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	7	from	Changes	0:6	arg1	composition					15:25	composition	15:25	composition	15:25	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	5	8	theme	Cell	569:572	arg1	polysaccharides					579:593	Cell wall polysaccharides	569:593	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH	569:651	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	2	9	theme	artemisinin	271:281	arg1	synthesis					283:291	the artemisinin synthesis	267:291	the artemisinin synthesis	267:291	Moderate salt stress has been proved to increase the artemisinin synthesis by the plant.					
31362136	4	10	theme	membranes	558:566	arg1	damage					539:544	high damage	534:544	high damage of cellular membranes	534:566	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	7	11	contain	have	964:967	arg2	role					982:985	an important role	969:985	an important role	969:985	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	7	11	contain	have	964:967	arg1	arabinans					954:962	arabinans	954:962	arabinans	954:962	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	6	12	theme	main	731:734	arg1	changes					736:742	The main changes	727:742	The main changes	727:742	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	5	13	theme	wall	574:577	arg1	polysaccharides					579:593	Cell wall polysaccharides	569:593	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH	569:651	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	4	14	theme	cellular	549:556	arg1	membranes					558:566	cellular membranes	549:566	cellular membranes	549:566	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	5	15	theme	monosaccharide	699:712	arg1	composition					714:724	monosaccharide composition	699:724	monosaccharide composition	699:724	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	8	16	mod	modified	1045:1052	arg3	contents					1095:1102	increased xylose contents	1078:1102	increased xylose contents	1078:1102	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	8	16	mod	modified	1045:1052	arg1	domain					1029:1034	Hemicellulose domain	1015:1034	Hemicellulose domain	1015:1034	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	6	17	theme	pectic	762:767	arg1	polysaccharides					769:783	the pectic polysaccharides	758:783	the pectic polysaccharides	758:783	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	5	18	from	modifications	668:680	arg1	yield					689:693	yield	689:693	yield	689:693	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	5	18	from	modifications	668:680	arg1	composition					714:724	monosaccharide composition	699:724	monosaccharide composition	699:724	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	3	19	theme	cell	406:409	arg1	polysaccharides					416:430	cell wall polysaccharides	406:430	cell wall polysaccharides	406:430	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	3	20	theme	study	323:327	arg1	aim					311:313	The aim	307:313	The aim of this study	307:327	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	0	21	from	structure	31:39	arg1	response					94:101	response	94:101	response to salt stress	94:116	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	21	from	structure	31:39	arg1	annua					85:89	Artemisia annua	75:89	Artemisia annua	75:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	6	22	from	increase	859:866	arg1	esterification					882:895	the methyl esterification	871:895	the methyl esterification	871:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	3	23	theme	wall	411:414	arg1	polysaccharides					416:430	cell wall polysaccharides	406:430	cell wall polysaccharides	406:430	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	7	24	theme	pectins	917:923	arg1	analyses					905:912	1H NMR analyses	898:912	1H NMR analyses of pectins	898:923	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	6	25	theme	chains	848:853	arg1	increase					859:866	increase	859:866	increase in the methyl esterification	859:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	25	theme	chains	848:853	arg1	increase					823:830	increase	823:830	increase of neutral side chains	823:853	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	25	theme	chains	848:853	arg1	increase					786:793	increase	786:793	increase of homogalacturonan domain	786:820	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	5	26	theme	aerial	610:615	arg1	parts					617:621	aerial parts	610:621	aerial parts with hot water, EDTA and NaOH	610:651	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	8	27	theme	xylose	1088:1093	arg1	contents					1095:1102	increased xylose contents	1078:1102	increased xylose contents	1078:1102	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	8	28	theme	salt	1060:1063	arg1	stress					1065:1070	salt stress	1060:1070	salt stress	1060:1070	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	6	29	from	increase	786:793	arg1	esterification					882:895	the methyl esterification	871:895	the methyl esterification	871:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	30	from	increase	823:830	arg1	esterification					882:895	the methyl esterification	871:895	the methyl esterification	871:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	8	31	theme	Hemicellulose	1015:1027	arg1	domain					1029:1034	Hemicellulose domain	1015:1034	Hemicellulose domain	1015:1034	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	7	32	theme	1H	898:899	arg1	analyses					905:912	1H NMR analyses	898:912	1H NMR analyses of pectins	898:923	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	1	33	theme	artemisinin	173:183	arg1	isolation					160:168	isolation	160:168	isolation of artemisinin, a potent antimalarial compound	160:215	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	9	34	theme	annua	1162:1166	arg1	wall					1151:1154	the cell wall	1142:1154	the cell wall of A. annua	1142:1166	The results indicated adaptations in the cell wall of A. annua under salt stress.					
31362136	7	35	theme	NMR	901:903	arg1	analyses					905:912	1H NMR analyses	898:912	1H NMR analyses of pectins	898:923	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	3	36	theme	A.	435:436	arg1	annua					438:442	A. annua	435:442	A. annua	435:442	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	4	37	theme	high	534:537	arg1	damage					539:544	high damage	534:544	high damage of cellular membranes	534:566	Plants subjected to salt stress displayed reduction in the biomass and length and showed high damage of cellular membranes.					
31362136	0	38	theme	salt	106:109	arg1	stress					111:116	salt stress	106:116	salt stress	106:116	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	6	39	theme	side	843:846	arg1	chains					848:853	neutral side chains	835:853	neutral side chains	835:853	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	3	40	theme	annua	438:442	arg1	parameters					391:400	physiological parameters	377:400	physiological parameters	377:400	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	3	40	theme	annua	438:442	arg1	polysaccharides					416:430	cell wall polysaccharides	406:430	cell wall polysaccharides	406:430	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	6	41	theme	neutral	835:841	arg1	chains					848:853	neutral side chains	835:853	neutral side chains	835:853	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	2	42	theme	salt	227:230	arg1	stress					232:237	Moderate salt stress	218:237	Moderate salt stress	218:237	Moderate salt stress has been proved to increase the artemisinin synthesis by the plant.					
31362136	1	43	theme	potent	188:193	arg1	compound					208:215	a potent antimalarial compound	186:215	a potent antimalarial compound	186:215	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	1	43	theme	potent	188:193	arg1	artemisinin					173:183	artemisinin	173:183	artemisinin	173:183	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	3	44	theme	physiological	377:389	arg1	parameters					391:400	physiological parameters	377:400	physiological parameters	377:400	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	2	45	theme	Moderate	218:225	arg1	stress					232:237	Moderate salt stress	218:237	Moderate salt stress	218:237	Moderate salt stress has been proved to increase the artemisinin synthesis by the plant.					
31362136	7	46	theme	important	972:980	arg1	role					982:985	an important role	969:985	an important role	969:985	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	1	47	theme	antimalarial	195:206	arg1	compound					208:215	a potent antimalarial compound	186:215	a potent antimalarial compound	186:215	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	1	47	theme	antimalarial	195:206	arg1	artemisinin					173:183	artemisinin	173:183	artemisinin	173:183	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	3	48	theme	salt	362:365	arg1	stress					367:372	salt stress	362:372	salt stress	362:372	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	9	49	theme	cell	1146:1149	arg1	wall					1151:1154	the cell wall	1142:1154	the cell wall of A. annua	1142:1166	The results indicated adaptations in the cell wall of A. annua under salt stress.					
31362136	0	50	theme	wall	49:52	arg1	polysaccharides					54:68	cell wall polysaccharides	44:68	cell wall polysaccharides from Artemisia annua	44:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	3	51	from	influence	349:357	arg1	parameters					391:400	physiological parameters	377:400	physiological parameters	377:400	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	3	51	from	influence	349:357	arg1	polysaccharides					416:430	cell wall polysaccharides	406:430	cell wall polysaccharides	406:430	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	3	52	theme	stress	367:372	arg1	influence					349:357	the influence	345:357	the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua	345:442	The aim of this study was to evaluate the influence of salt stress on physiological parameters and cell wall polysaccharides of A. annua.					
31362136	7	53	theme	salt	1002:1005	arg1	stress					1007:1012	salt stress	1002:1012	salt stress	1002:1012	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	9	54	from	adaptations	1127:1137	arg1	wall					1151:1154	the cell wall	1142:1154	the cell wall of A. annua	1142:1166	The results indicated adaptations in the cell wall of A. annua under salt stress.					
31362136	5	55	with	parts	617:621	arg1	NaOH					648:651	NaOH	648:651	NaOH	648:651	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	5	55	with	parts	617:621	arg1	EDTA					639:642	EDTA	639:642	EDTA	639:642	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	5	55	with	parts	617:621	arg1	water					632:636	hot water	628:636	hot water	628:636	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	5	56	dep	yield	689:693	arg1	the					685:687	the	685:687	the	685:687	Cell wall polysaccharides extracted from aerial parts with hot water, EDTA and NaOH also exhibited modifications in the yield and monosaccharide composition.					
31362136	6	57	theme	domain	815:820	arg1	increase					859:866	increase	859:866	increase in the methyl esterification	859:895	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	57	theme	domain	815:820	arg1	increase					823:830	increase	823:830	increase of neutral side chains	823:853	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	6	57	theme	domain	815:820	arg1	increase					786:793	increase	786:793	increase of homogalacturonan domain	786:820	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	0	58	dep	composition	15:25	arg1	the					11:13	the	11:13	the	11:13	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	6	59	theme	homogalacturonan	798:813	arg1	domain					815:820	homogalacturonan domain	798:820	homogalacturonan domain	798:820	The main changes were found in the pectic polysaccharides: increase of homogalacturonan domain, increase of neutral side chains and increase in the methyl esterification.					
31362136	1	60	theme	Artemisia	119:127	arg1	annua					129:133	Artemisia annua	119:133	Artemisia annua	119:133	Artemisia annua is cultivated mainly for isolation of artemisinin, a potent antimalarial compound.					
31362136	8	61	theme	increased	1078:1086	arg1	contents					1095:1102	increased xylose contents	1078:1102	increased xylose contents	1078:1102	Hemicellulose domain was also modified under salt stress, with increased xylose contents.					
31362136	0	62	theme	polysaccharides	54:68	arg1	structure					31:39	structure	31:39	structure	31:39	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	62	theme	polysaccharides	54:68	arg1	composition					15:25	composition	15:25	composition	15:25	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	63	from	annua	85:89	arg1	structure					31:39	structure	31:39	structure	31:39	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	63	from	annua	85:89	arg1	composition					15:25	composition	15:25	composition	15:25	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	63	from	annua	85:89	arg1	polysaccharides					54:68	cell wall polysaccharides	44:68	cell wall polysaccharides from Artemisia annua	44:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	7	64	with	coping	990:995	arg1	stress					1007:1012	salt stress	1002:1012	salt stress	1002:1012	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
31362136	9	65	theme	A.	1159:1160	arg1	annua					1162:1166	A. annua	1159:1166	A. annua	1159:1166	The results indicated adaptations in the cell wall of A. annua under salt stress.					
31362136	0	66	theme	cell	44:47	arg1	polysaccharides					54:68	cell wall polysaccharides	44:68	cell wall polysaccharides from Artemisia annua	44:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	67	from	composition	15:25	arg1	response					94:101	response	94:101	response to salt stress	94:116	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	0	67	from	composition	15:25	arg1	annua					85:89	Artemisia annua	75:89	Artemisia annua	75:89	Changes in the composition and structure of cell wall polysaccharides from Artemisia annua in response to salt stress.					
31362136	7	68	theme	A.	944:945	arg1	annua					947:951	A. annua	944:951	A. annua	944:951	1H NMR analyses of pectins indicated that for A. annua, arabinans have an important role in coping with salt stress.					
30742920	8	0	theme	myeloid	1199:1205	arg1	factor					1223:1228	myeloid differentiation factor	1199:1228	myeloid differentiation factor (MyD88)	1199:1236	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	0	theme	myeloid	1199:1205	arg1	TRAF-6					1294:1299	TRAF-6	1294:1299	TRAF-6	1294:1299	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	0	theme	myeloid	1199:1205	arg1	receptor-4					1179:1188	toll-like receptor-4	1169:1188	toll-like receptor-4 (TLR-4)	1169:1196	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	0	theme	myeloid	1199:1205	arg1	MyD88					1231:1235	MyD88	1231:1235	MyD88	1231:1235	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	1	theme	receptor-4	1179:1188	arg1	expression					1155:1164	the mRNA and protein expression	1134:1164	expression	1155:1164	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	3	2	theme	polysaccharide	472:485	arg1	content					448:454	the sulfate ester content	430:454	the sulfate ester content of the sulfated polysaccharide	430:485	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	3	2	theme	polysaccharide	472:485	arg1	%					507:507	8.8%	504:507	8.8%	504:507	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	9	3	theme	anti-inflammatory	1473:1489	arg1	effect					1491:1496	an anti-inflammatory effect	1470:1496	an anti-inflammatory effect	1470:1496	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	8	4	theme	mRNA	1138:1141	arg1	expression					1155:1164	the mRNA and protein expression	1134:1164	expression	1155:1164	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	6	5	theme	LPS-stimulated	869:882	arg1	cytotoxicity					884:895	LPS-stimulated cytotoxicity	869:895	LPS-stimulated cytotoxicity	869:895	The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity.					
30742920	4	6	theme	sulfated	545:552	arg1	polysaccharide					554:567	the sulfated polysaccharide	541:567	the sulfated polysaccharide	541:567	Structure analysis showed that the sulfated polysaccharide comprised of 1,4-linked-α-D-Galp3S, 1,2-linked-α-D-Xylp and 1,3-linked-β-D-GalpA residues, respectively.					
30742920	0	7	from	Characterization	0:15	arg1	Okamura					114:120	Okamura	114:120	Okamura	114:120	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	5	8	theme	anti-inflammatory	678:694	arg1	effects					696:702	Its anti-inflammatory effects	674:702	Its anti-inflammatory effects	674:702	Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells.					
30742920	6	9	theme	THP-1	849:853	arg1	cells					855:859	the THP-1 cells	845:859	the THP-1 cells	845:859	The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity.					
30742920	7	10	theme	sulfated	927:934	arg1	polysaccharide					936:949	sulfated polysaccharide	927:949	sulfated polysaccharide	927:949	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	1	11	theme	molecular	279:287	arg1	weight					289:294	molecular weight	279:294	molecular weight of 28,807 Da	279:307	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
30742920	8	12	theme	differentiation	1207:1221	arg1	factor					1223:1228	myeloid differentiation factor	1199:1228	myeloid differentiation factor (MyD88)	1199:1236	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	12	theme	differentiation	1207:1221	arg1	TRAF-6					1294:1299	TRAF-6	1294:1299	TRAF-6	1294:1299	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	12	theme	differentiation	1207:1221	arg1	receptor-4					1179:1188	toll-like receptor-4	1169:1188	toll-like receptor-4 (TLR-4)	1169:1196	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	12	theme	differentiation	1207:1221	arg1	MyD88					1231:1235	MyD88	1231:1235	MyD88	1231:1235	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	7	13	theme	LPS-treated	1007:1017	arg1	cells					1019:1023	LPS-treated cells	1007:1023	LPS-treated cells	1007:1023	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	7	14	from	reduction	977:985	arg1	cells					1019:1023	LPS-treated cells	1007:1023	LPS-treated cells	1007:1023	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	6	15	from	concentration	804:816	arg1	polysaccharide					784:797	The sulfated polysaccharide	771:797	The sulfated polysaccharide at a concentration of 5 μg/mL	771:827	The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity.					
30742920	9	16	theme	TLR4	1506:1509	arg1	pathway					1521:1527	the TLR4 signaling pathway	1502:1527	the TLR4 signaling pathway	1502:1527	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	9	17	theme	sulfated	1358:1365	arg1	polysaccharide					1367:1380	the sulfated polysaccharide	1354:1380	the sulfated polysaccharide	1354:1380	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	8	18	theme	receptor-associated	1264:1282	arg1	receptor-4					1179:1188	toll-like receptor-4	1169:1188	toll-like receptor-4 (TLR-4)	1169:1196	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	18	theme	receptor-associated	1264:1282	arg1	factor-6					1284:1291	tumor necrosis factor receptor-associated factor-6	1242:1291	tumor necrosis factor receptor-associated factor-6	1242:1291	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	7	19	theme	production	993:1002	arg1	reduction					977:985	a significant reduction	963:985	a significant reduction of NO production in LPS-treated cells	963:1023	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	9	20	theme	signaling	1511:1519	arg1	pathway					1521:1527	the TLR4 signaling pathway	1502:1527	the TLR4 signaling pathway	1502:1527	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	6	21	theme	5 μg/mL	821:827	arg1	concentration					804:816	a concentration	802:816	a concentration of 5 μg/mL	802:827	The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity.					
30742920	8	22	theme	LPS-stimulated	1305:1318	arg1	cells					1326:1330	LPS-stimulated THP-1 cells	1305:1330	LPS-stimulated THP-1 cells	1305:1330	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	23	theme	THP-1	1320:1324	arg1	cells					1326:1330	LPS-stimulated THP-1 cells	1305:1330	LPS-stimulated THP-1 cells	1305:1330	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	24	theme	sulfated	1075:1082	arg1	5 μg/mL					1100:1106	5 μg/mL	1100:1106	5 μg/mL	1100:1106	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	24	theme	sulfated	1075:1082	arg1	polysaccharide					1084:1097	The sulfated polysaccharide	1071:1097	The sulfated polysaccharide (5 μg/mL)	1071:1107	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	25	theme	protein	1147:1153	arg1	expression					1155:1164	the mRNA and protein expression	1134:1164	expression	1155:1164	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	1	26	theme	28,807 Da	299:307	arg1	weight					289:294	molecular weight	279:294	molecular weight of 28,807 Da	279:307	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
30742920	5	27	theme	human	740:744	arg1	cells					764:768	LPS-stimulated human monocytic (THP-1) cells	725:768	LPS-stimulated human monocytic (THP-1) cells	725:768	Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells.					
30742920	7	28	theme	NO	990:991	arg1	production					993:1002	NO production	990:1002	NO production	990:1002	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	3	29	theme	sulfate	434:440	arg1	content					448:454	the sulfate ester content	430:454	the sulfate ester content of the sulfated polysaccharide	430:485	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	3	29	theme	sulfate	434:440	arg1	%					507:507	8.8%	504:507	8.8%	504:507	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	0	30	theme	anti-inflammatory	21:37	arg1	effects					39:45	anti-inflammatory effects	21:45	anti-inflammatory effects	21:45	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	5	31	theme	monocytic	746:754	arg1	cells					764:768	LPS-stimulated human monocytic (THP-1) cells	725:768	LPS-stimulated human monocytic (THP-1) cells	725:768	Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells.					
30742920	2	32	theme	monosaccharide	314:327	arg1	composition					329:339	Its monosaccharide composition	310:339	Its monosaccharide composition	310:339	Its monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%).					
30742920	3	33	theme	ester	442:446	arg1	content					448:454	the sulfate ester content	430:454	the sulfate ester content of the sulfated polysaccharide	430:485	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	3	33	theme	ester	442:446	arg1	%					507:507	8.8%	504:507	8.8%	504:507	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	0	34	theme	sulfated	50:57	arg1	polysaccharide					59:72	sulfated polysaccharide	50:72	sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura	50:120	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	3	35	theme	sulfated	463:470	arg1	polysaccharide					472:485	the sulfated polysaccharide	459:485	the sulfated polysaccharide	459:485	And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%.					
30742920	2	36	theme	galacturonic	398:409	arg1	acid					411:414	galacturonic acid	398:414	galacturonic acid (19.76%)	398:423	Its monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%).					
30742920	2	36	theme	galacturonic	398:409	arg1	%					422:422	19.76%	417:422	19.76%	417:422	Its monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%).					
30742920	9	37	theme	LPS-induced	1426:1436	arg1	toxicity					1443:1450	LPS-induced cell toxicity	1426:1450	LPS-induced cell toxicity	1426:1450	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	5	38	theme	THP-1	757:761	arg1	cells					764:768	LPS-stimulated human monocytic (THP-1) cells	725:768	LPS-stimulated human monocytic (THP-1) cells	725:768	Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells.					
30742920	9	39	theme	cell	1438:1441	arg1	toxicity					1443:1450	LPS-induced cell toxicity	1426:1450	LPS-induced cell toxicity	1426:1450	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	8	40	theme	toll-like	1169:1177	arg1	factor-6					1284:1291	tumor necrosis factor receptor-associated factor-6	1242:1291	tumor necrosis factor receptor-associated factor-6	1242:1291	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	40	theme	toll-like	1169:1177	arg1	TLR-4					1191:1195	TLR-4	1191:1195	TLR-4	1191:1195	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	40	theme	toll-like	1169:1177	arg1	receptor-4					1179:1188	toll-like receptor-4	1169:1188	toll-like receptor-4 (TLR-4)	1169:1196	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	8	40	theme	toll-like	1169:1177	arg1	factor					1223:1228	myeloid differentiation factor	1199:1228	myeloid differentiation factor (MyD88)	1199:1236	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	9	41	theme	good	1402:1405	arg1	protection					1407:1416	a good protection	1400:1416	a good protection against LPS-induced cell toxicity	1400:1450	These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.					
30742920	0	42	theme	polysaccharide	59:72	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	0	42	theme	polysaccharide	59:72	arg1	effects					39:45	anti-inflammatory effects	21:45	anti-inflammatory effects	21:45	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	4	43	theme	Structure	510:518	arg1	analysis					520:527	Structure analysis	510:527	Structure analysis	510:527	Structure analysis showed that the sulfated polysaccharide comprised of 1,4-linked-α-D-Galp3S, 1,2-linked-α-D-Xylp and 1,3-linked-β-D-GalpA residues, respectively.					
30742920	1	44	with	polysaccharide	259:272	arg1	weight					289:294	molecular weight	279:294	molecular weight of 28,807 Da	279:307	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
30742920	1	45	theme	present	130:136	arg1	study					138:142	the present study	126:142	the present study	126:142	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
30742920	7	46	theme	significant	965:975	arg1	reduction					977:985	a significant reduction	963:985	a significant reduction of NO production in LPS-treated cells	963:1023	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	6	47	theme	sulfated	775:782	arg1	polysaccharide					784:797	The sulfated polysaccharide	771:797	The sulfated polysaccharide at a concentration of 5 μg/mL	771:827	The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity.					
30742920	8	48	from	expression	1155:1164	arg1	cells					1326:1330	LPS-stimulated THP-1 cells	1305:1330	LPS-stimulated THP-1 cells	1305:1330	The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells.					
30742920	5	49	theme	LPS-stimulated	725:738	arg1	cells					764:768	LPS-stimulated human monocytic (THP-1) cells	725:768	LPS-stimulated human monocytic (THP-1) cells	725:768	Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells.					
30742920	0	50	from	Okamura	114:120	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	0	50	from	Okamura	114:120	arg1	effects					39:45	anti-inflammatory effects	21:45	anti-inflammatory effects	21:45	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	0	50	from	Okamura	114:120	arg1	polysaccharide					59:72	sulfated polysaccharide	50:72	sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura	50:120	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	4	51	dep	1,4-linked-α-D-Galp3S	582:602	arg1	residues					650:657	residues	650:657	residues	650:657	Structure analysis showed that the sulfated polysaccharide comprised of 1,4-linked-α-D-Galp3S, 1,2-linked-α-D-Xylp and 1,3-linked-β-D-GalpA residues, respectively.					
30742920	1	52	theme	crude	145:149	arg1	polysaccharides					151:165	crude polysaccharides	145:165	crude polysaccharides	145:165	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
30742920	0	53	from	effects	39:45	arg1	Okamura					114:120	Okamura	114:120	Okamura	114:120	Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura.					
30742920	7	54	theme	polysaccharide	936:949	arg1	addition					915:922	the addition	911:922	the addition of sulfated polysaccharide	911:949	Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related.					
30742920	1	55	theme	sulfated	250:257	arg1	polysaccharide					259:272	the sulfated polysaccharide	246:272	the sulfated polysaccharide with molecular weight of 28,807 Da	246:307	In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da.					
31353545	6	0	theme	broad	1164:1168	arg1	applications					1170:1181	the broad applications	1160:1181	the broad applications of these annatto dye yielding plant polysaccharides	1160:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	10	1	theme	twig	1634:1637	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	1	theme	twig	1634:1637	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	2	theme	rich	1665:1668	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	2	theme	rich	1665:1668	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	3	theme	potential	1732:1740	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	3	theme	potential	1732:1740	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	4	theme	orellana	1653:1660	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	4	theme	orellana	1653:1660	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	7	5	theme	mucilaginous	1264:1275	arg1	MPS					1294:1296	MPS	1294:1296	MPS	1294:1296	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	7	5	theme	mucilaginous	1264:1275	arg1	polysaccharides					1277:1291	The mucilaginous polysaccharides	1260:1291	The mucilaginous polysaccharides (MPS) from plant sources	1260:1316	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	1	6	theme	mucilaginous	170:181	arg1	polysaccharides					183:197	adhesive mucilaginous polysaccharides	161:197	adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana	161:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	1	6	theme	mucilaginous	170:181	arg1	MPS					200:202	MPS	200:202	MPS	200:202	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	6	7	theme	polysaccharides	1219:1233	arg1	applications					1170:1181	the broad applications	1160:1181	the broad applications of these annatto dye yielding plant polysaccharides	1160:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	10	8	theme	foods	1870:1874	arg1	consistency					1876:1886	dairy foods consistency	1864:1886	dairy foods consistency	1864:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	2	9	theme	phenolic	597:604	arg1	content					606:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	1	10	theme	vegetative	210:219	arg1	parts					221:225	vegetative parts	210:225	vegetative parts of Bixa orellana	210:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	2	11	theme	protein	646:652	arg1	content					654:660	36.9 ± 0.52 mg/100 g total protein content	619:660	36.9 ± 0.52 mg/100 g total protein content	619:660	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	12	theme	MPS	1690:1692	arg1	characterization					1694:1709	the MPS characterization	1686:1709	the MPS characterization	1686:1709	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	2	13	theme	36.9 ± 0.52 mg/100 g	619:638	arg1	content					654:660	36.9 ± 0.52 mg/100 g total protein content	619:660	36.9 ± 0.52 mg/100 g total protein content	619:660	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	4	14	theme	MPS	799:801	arg1	characteristics					780:794	The sugar composition characteristics	758:794	The sugar composition characteristics of MPS by gas liquid chromatography	758:830	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	3	15	theme	the	689:691	arg1	MPS					700:702	the leaves MPS	689:702	the leaves MPS	689:702	In contrasted with twigs, the leaves MPS demonstrated two folds increment in phenolic content.					
31353545	5	16	theme	leaves	995:1000	arg1	extract					984:990	The MPS extract	976:990	The MPS extract of leaves	976:1000	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	2	17	theme	uronic	548:553	arg1	substance					560:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	7	18	dep	APPLICATIONS	1246:1257	arg1	used					1328:1331	used	1328:1331	are being used for different applications in food, pharma, and cosmetic enterprises	1318:1400	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	4	19	theme	sugar	762:766	arg1	characteristics					780:794	The sugar composition characteristics	758:794	The sugar composition characteristics of MPS by gas liquid chromatography	758:830	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	7	20	theme	plant	1304:1308	arg1	sources					1310:1316	plant sources	1304:1316	plant sources	1304:1316	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	4	21	from	content	858:864	arg1	leaf					970:973	leaf	970:973	leaf	970:973	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	2	22	theme	10.6 ± 0.98 g/100 g	571:589	arg1	content					606:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	23	dep	B.	1768:1769	arg1	orellana					1771:1778	orellana	1771:1778	orellana	1771:1778	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	24	theme	B.	1768:1769	arg1	MPS					1780:1782	B. orellana MPS	1768:1782	B. orellana MPS	1768:1782	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	24	theme	B.	1768:1769	arg1	ingredients					1798:1808	functional ingredients	1787:1808	functional ingredients	1787:1808	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	5	25	theme	radical	1059:1065	arg1	ability					1078:1084	free radical scavenging ability	1054:1084	free radical scavenging ability	1054:1084	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	2	26	theme	sugar	477:481	arg1	content					483:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	0	27	from	parts	45:49	arg1	characterization					78:93	Their characterization	72:93	Their characterization	72:93	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	27	from	parts	45:49	arg1	potential					111:119	antioxidant potential	99:119	antioxidant potential	99:119	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	27	from	parts	45:49	arg1	polysaccharides					13:27	Mucilaginous polysaccharides	0:27	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.	0:120	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	4	28	theme	gas	806:808	arg1	chromatography					817:830	gas liquid chromatography	806:830	gas liquid chromatography	806:830	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	2	29	theme	%	390:390	arg1	moisture					392:399	57.7% ± 3.2% moisture	379:399	57.7% ± 3.2% moisture	379:399	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	0	30	theme	Mucilaginous	0:11	arg1	characterization					78:93	Their characterization	72:93	Their characterization	72:93	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	30	theme	Mucilaginous	0:11	arg1	potential					111:119	antioxidant potential	99:119	antioxidant potential	99:119	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	30	theme	Mucilaginous	0:11	arg1	polysaccharides					13:27	Mucilaginous polysaccharides	0:27	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.	0:120	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	1	31	theme	proximate	271:279	arg1	analysis					281:288	proximate analysis	271:288	proximate analysis	271:288	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	2	32	theme	23.6 ± 0.07 g/100 g	448:466	arg1	content					483:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	33	theme	nourishment	1829:1839	arg1	industry					1841:1848	nourishment industry	1829:1848	nourishment industry for expanding dairy foods consistency	1829:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	5	34	theme	antioxidant	1029:1039	arg1	activity					1041:1048	a dose-dependent antioxidant activity	1012:1048	a dose-dependent antioxidant activity	1012:1048	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	7	35	theme	cosmetic	1381:1388	arg1	enterprises					1390:1400	cosmetic enterprises	1381:1400	cosmetic enterprises	1381:1400	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	2	36	theme	pentose	511:517	arg1	content					519:525	14.5 ± 2.0 g/100 g pentose content	492:525	14.5 ± 2.0 g/100 g pentose content	492:525	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	2	37	theme	aggregate	422:430	arg1	content					439:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	7	38	theme	PRACTICAL	1236:1244	arg1	APPLICATIONS					1246:1257	PRACTICAL APPLICATIONS	1236:1257	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.	1236:1401	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	0	39	dep	Bixa	54:57	arg1	L.					68:69	Bixa orellana L.	54:69	Bixa orellana L.	54:69	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	40	theme	Bixa	54:57	arg1	parts					45:49	vegetative parts	34:49	vegetative parts of Bixa orellana L.	34:69	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	6	41	theme	dye	1200:1202	arg1	polysaccharides					1219:1233	these annatto dye yielding plant polysaccharides	1186:1233	these annatto dye yielding plant polysaccharides	1186:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	3	42	theme	phenolic	740:747	arg1	content					749:755	phenolic content	740:755	phenolic content	740:755	In contrasted with twigs, the leaves MPS demonstrated two folds increment in phenolic content.					
31353545	7	43	used	used	1328:1331	arg2	polysaccharides					1277:1291	The mucilaginous polysaccharides	1260:1291	The mucilaginous polysaccharides (MPS) from plant sources	1260:1316	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	7	43	used	used	1328:1331	arg2	MPS					1294:1296	MPS	1294:1296	MPS	1294:1296	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	8	44	dep	Nowadays	1403:1410	arg1	there					1412:1416	there	1412:1416	there	1412:1416	Nowadays there is a developing enthusiasm for MPS for their dietary and medicinal advantages.					
31353545	10	45	theme	Bixa	1648:1651	arg1	orellana					1653:1660	Bixa orellana	1648:1660	Bixa orellana	1648:1660	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	2	46	theme	%	383:383	arg1	%					390:390	57.7% ± 3.2%	379:390	57.7% ± 3.2% moisture	379:399	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	1	47	theme	Bixa	230:233	arg1	orellana					235:242	Bixa orellana	230:242	Bixa orellana	230:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	7	48	theme	different	1337:1345	arg1	applications					1347:1358	different applications	1337:1358	different applications in food, pharma, and cosmetic enterprises	1337:1400	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	4	49	theme	galactose	935:943	arg1	content					858:864	a noteworthy content	845:864	a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf	845:973	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	10	50	theme	dairy	1864:1868	arg1	foods					1870:1874	dairy foods	1864:1874	dairy foods consistency	1864:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	9	51	theme	dietary	1510:1516	arg1	polysaccharides					1518:1532	Particularly dietary polysaccharides	1497:1532	Particularly dietary polysaccharides	1497:1532	Particularly dietary polysaccharides are known to evoke immunomodulatory and anti-inflammatory properties.					
31353545	1	52	theme	adhesive	161:168	arg1	polysaccharides					183:197	adhesive mucilaginous polysaccharides	161:197	adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana	161:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	1	52	theme	adhesive	161:168	arg1	MPS					200:202	MPS	200:202	MPS	200:202	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	7	53	from	applications	1347:1358	arg1	pharma					1369:1374	pharma	1369:1374	pharma	1369:1374	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	7	53	from	applications	1347:1358	arg1	food					1363:1366	food	1363:1366	food	1363:1366	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	7	53	from	applications	1347:1358	arg1	enterprises					1390:1400	cosmetic enterprises	1381:1400	cosmetic enterprises	1381:1400	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	2	54	theme	ethanol	322:328	arg1	mucilage					343:350	The ethanol precipitated mucilage	318:350	The ethanol precipitated mucilage	318:350	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	55	theme	MPS	1681:1683	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	55	theme	MPS	1681:1683	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	4	56	theme	mannose	900:906	arg1	content					858:864	a noteworthy content	845:864	a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf	845:973	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	9	57	theme	immunomodulatory	1553:1568	arg1	properties					1592:1601	immunomodulatory and anti-inflammatory properties	1553:1601	immunomodulatory and anti-inflammatory properties	1553:1601	Particularly dietary polysaccharides are known to evoke immunomodulatory and anti-inflammatory properties.					
31353545	6	58	theme	plant	1213:1217	arg1	polysaccharides					1219:1233	these annatto dye yielding plant polysaccharides	1186:1233	these annatto dye yielding plant polysaccharides	1186:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	9	59	theme	anti-inflammatory	1574:1590	arg1	properties					1592:1601	immunomodulatory and anti-inflammatory properties	1553:1601	immunomodulatory and anti-inflammatory properties	1553:1601	Particularly dietary polysaccharides are known to evoke immunomodulatory and anti-inflammatory properties.					
31353545	2	60	theme	total	591:595	arg1	content					606:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	10.6 ± 0.98 g/100 g total phenolic content	571:612	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	61	theme	characterization	1694:1709	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	61	theme	characterization	1694:1709	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	5	62	theme	MPS	980:982	arg1	extract					984:990	The MPS extract	976:990	The MPS extract of leaves	976:1000	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	2	63	theme	total	640:644	arg1	content					654:660	36.9 ± 0.52 mg/100 g total protein content	619:660	36.9 ± 0.52 mg/100 g total protein content	619:660	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	4	64	theme	composition	768:778	arg1	characteristics					780:794	The sugar composition characteristics	758:794	The sugar composition characteristics of MPS by gas liquid chromatography	758:830	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	1	65	from	parts	221:225	arg1	polysaccharides					183:197	adhesive mucilaginous polysaccharides	161:197	adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana	161:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	1	65	from	parts	221:225	arg1	MPS					200:202	MPS	200:202	MPS	200:202	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	4	66	theme	xylose	869:874	arg1	content					858:864	a noteworthy content	845:864	a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf	845:973	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	7	67	from	sources	1310:1316	arg1	MPS					1294:1296	MPS	1294:1296	MPS	1294:1296	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	7	67	from	sources	1310:1316	arg1	polysaccharides					1277:1291	The mucilaginous polysaccharides	1260:1291	The mucilaginous polysaccharides (MPS) from plant sources	1260:1316	PRACTICAL APPLICATIONS: The mucilaginous polysaccharides (MPS) from plant sources are being used for different applications in food, pharma, and cosmetic enterprises.					
31353545	1	68	theme	study	138:142	arg1	aim					126:128	The aim	122:128	The aim of this study	122:142	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	2	69	theme	9.37 ± 0.56 g/100 g	528:546	arg1	substance					560:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	0	70	theme	antioxidant	99:109	arg1	potential					111:119	antioxidant potential	99:119	antioxidant potential	99:119	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	70	theme	antioxidant	99:109	arg1	polysaccharides					13:27	Mucilaginous polysaccharides	0:27	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.	0:120	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	5	71	theme	free	1054:1057	arg1	radical					1059:1065	free radical	1054:1065	free radical scavenging ability	1054:1084	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	2	72	theme	acid	555:558	arg1	substance					560:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	9.37 ± 0.56 g/100 g uronic acid substance	528:568	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	8	73	theme	developing	1423:1432	arg1	enthusiasm					1434:1443	a developing enthusiasm	1421:1443	a developing enthusiasm for MPS for their dietary and medicinal advantages	1421:1494	Nowadays there is a developing enthusiasm for MPS for their dietary and medicinal advantages.					
31353545	2	74	theme	reducing	468:475	arg1	content					483:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	23.6 ± 0.07 g/100 g reducing sugar content	448:489	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	10	75	theme	antioxidant	1720:1730	arg1	potential					1732:1740	antioxidant potential	1720:1740	antioxidant potential	1720:1740	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	0	76	theme	vegetative	34:43	arg1	parts					45:49	vegetative parts	34:49	vegetative parts of Bixa orellana L.	34:69	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	5	77	theme	dose-dependent	1014:1027	arg1	activity					1041:1048	a dose-dependent antioxidant activity	1012:1048	a dose-dependent antioxidant activity	1012:1048	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	6	78	theme	new	1126:1128	arg1	avenue					1130:1135	a new avenue	1124:1135	a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides	1124:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	4	79	theme	liquid	810:815	arg1	chromatography					817:830	gas liquid chromatography	806:830	gas liquid chromatography	806:830	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	2	80	theme	starch	432:437	arg1	content					439:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	1	81	theme	antioxidant	294:304	arg1	potentials					306:315	antioxidant potentials	294:315	antioxidant potentials	294:315	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	2	82	theme	14.5 ± 2.0 g/100 g	492:509	arg1	content					519:525	14.5 ± 2.0 g/100 g pentose content	492:525	14.5 ± 2.0 g/100 g pentose content	492:525	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	8	83	theme	dietary	1463:1469	arg1	advantages					1485:1494	their dietary and medicinal advantages	1457:1494	their dietary and medicinal advantages	1457:1494	Nowadays there is a developing enthusiasm for MPS for their dietary and medicinal advantages.					
31353545	2	84	theme	31.7 ± 1.61 g/100 g	402:420	arg1	content					439:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	31.7 ± 1.61 g/100 g aggregate starch content	402:445	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	6	85	theme	yielding	1204:1211	arg1	polysaccharides					1219:1233	these annatto dye yielding plant polysaccharides	1186:1233	these annatto dye yielding plant polysaccharides	1186:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	10	86	theme	leaf	1625:1628	arg1	parts					1639:1643	the leaf and twig parts	1621:1643	parts	1639:1643	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	86	theme	leaf	1625:1628	arg1	sources					1670:1676	rich sources	1665:1676	rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency	1665:1886	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	8	87	theme	medicinal	1475:1483	arg1	advantages					1485:1494	their dietary and medicinal advantages	1457:1494	their dietary and medicinal advantages	1457:1494	Nowadays there is a developing enthusiasm for MPS for their dietary and medicinal advantages.					
31353545	10	88	theme	functional	1787:1796	arg1	MPS					1780:1782	B. orellana MPS	1768:1782	B. orellana MPS	1768:1782	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	10	88	theme	functional	1787:1796	arg1	ingredients					1798:1808	functional ingredients	1787:1808	functional ingredients	1787:1808	Here, we present the leaf and twig parts of Bixa orellana as rich sources of MPS, the MPS characterization and also antioxidant potential, which could substantiate B. orellana MPS as functional ingredients and furthermore in nourishment industry for expanding dairy foods consistency.					
31353545	5	89	theme	scavenging	1067:1076	arg1	ability					1078:1084	free radical scavenging ability	1054:1084	free radical scavenging ability	1054:1084	The MPS extract of leaves exhibited a dose-dependent antioxidant activity and free radical scavenging ability.					
31353545	6	90	theme	annatto	1192:1198	arg1	polysaccharides					1219:1233	these annatto dye yielding plant polysaccharides	1186:1233	these annatto dye yielding plant polysaccharides	1186:1233	The data obtained in this study open a new avenue to further investigate the broad applications of these annatto dye yielding plant polysaccharides.					
31353545	4	91	theme	noteworthy	847:856	arg1	content					858:864	a noteworthy content	845:864	a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf	845:973	The sugar composition characteristics of MPS by gas liquid chromatography demonstrated a noteworthy content of xylose (163.5 ± 6.6 mg/100 g), mannose (17.2 ± 0.6 mg/100 g), and galactose (13.7 ± 0.4 mg/100 g) in leaf.					
31353545	2	92	theme	precipitated	330:341	arg1	mucilage					343:350	The ethanol precipitated mucilage	318:350	The ethanol precipitated mucilage	318:350	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	3	93	dep	the	689:691	arg1	leaves					693:698	leaves	693:698	leaves	693:698	In contrasted with twigs, the leaves MPS demonstrated two folds increment in phenolic content.					
31353545	0	94	dep	polysaccharides	13:27	arg1	characterization					78:93	Their characterization	72:93	Their characterization	72:93	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	94	dep	polysaccharides	13:27	arg1	potential					111:119	antioxidant potential	99:119	antioxidant potential	99:119	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	0	94	dep	polysaccharides	13:27	arg1	polysaccharides					13:27	Mucilaginous polysaccharides	0:27	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.	0:120	Mucilaginous polysaccharides from vegetative parts of Bixa orellana L.: Their characterization and antioxidant potential.					
31353545	3	95	from	increment	727:735	arg1	content					749:755	phenolic content	740:755	phenolic content	740:755	In contrasted with twigs, the leaves MPS demonstrated two folds increment in phenolic content.					
31353545	1	96	theme	orellana	235:242	arg1	parts					221:225	vegetative parts	210:225	vegetative parts of Bixa orellana	210:242	The aim of this study was to extricate adhesive mucilaginous polysaccharides (MPS) from vegetative parts of Bixa orellana and furthermore to explore proximate analysis and antioxidant potentials.					
31353545	2	97	theme	57.7	379:382	arg1	%					390:390	57.7% ± 3.2%	379:390	57.7% ± 3.2% moisture	379:399	The ethanol precipitated mucilage concentrates of leaf holds 57.7% ± 3.2% moisture, 31.7 ± 1.61 g/100 g aggregate starch content, 23.6 ± 0.07 g/100 g reducing sugar content, 14.5 ± 2.0 g/100 g pentose content, 9.37 ± 0.56 g/100 g uronic acid substance, 10.6 ± 0.98 g/100 g total phenolic content, and 36.9 ± 0.52 mg/100 g total protein content.					
31353545	3	98	dep	two	717:719	arg1	folds					721:725	folds	721:725	folds increment in phenolic content	721:755	In contrasted with twigs, the leaves MPS demonstrated two folds increment in phenolic content.					
30235811	0	0	theme	Near-Infrared	94:106	arg1	Imaging					122:128	Near-Infrared Hyperspectral Imaging	94:128	Near-Infrared Hyperspectral Imaging	94:128	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	5	1	theme	sample	811:816	arg1	spectra					826:832	sample average spectra	811:832	sample average spectra calculated by preprocessed pixel-wise spectra	811:878	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	2	2	theme	total	394:398	arg1	polysaccharides					400:414	total polysaccharides	394:414	total polysaccharides	394:414	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	5	3	theme	average	818:824	arg1	spectra					826:832	sample average spectra	811:832	sample average spectra calculated by preprocessed pixel-wise spectra	811:878	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	1	4	from	determination	186:198	arg1	Hangbaiju					252:260	Hangbaiju	252:260	Hangbaiju	252:260	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	1	4	from	determination	186:198	arg1	morifolium					240:249	Chrysanthemum morifolium	226:249	Chrysanthemum morifolium (Hangbaiju)	226:261	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	6	5	theme	total	1097:1101	arg1	polysaccharides					1103:1117	total polysaccharides	1097:1117	total polysaccharides	1097:1117	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	7	6	theme	Chrysanthemum	1233:1245	arg1	flowers					1258:1264	Chrysanthemum morifolium flowers	1233:1264	Chrysanthemum morifolium flowers	1233:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	0	7	from	Determination	0:12	arg1	morifolium					77:86	Chrysanthemum morifolium	63:86	Chrysanthemum morifolium	63:86	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	4	8	theme	normal	668:673	arg1	SNV					684:686	SNV	684:686	SNV	684:686	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	4	8	theme	normal	668:673	arg1	variate					675:681	standard normal variate	659:681	standard normal variate (SNV)	659:687	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	0	9	theme	Hyperspectral	108:120	arg1	Imaging					122:128	Near-Infrared Hyperspectral Imaging	94:128	Near-Infrared Hyperspectral Imaging	94:128	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	8	10	theme	quality	1589:1595	arg1	determination					1597:1609	the quality determination	1585:1609	the quality determination of Chrysanthemum morifolium	1585:1637	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	5	11	used	used	773:776	arg2	machine					751:757	least squares-support vector machine	722:757	least squares-support vector machine (LS-SVM)	722:766	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	11	used	used	773:776	arg2	PLS					713:715	PLS	713:715	PLS	713:715	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	11	used	used	773:776	arg2	squares					704:710	Partial least squares	690:710	Partial least squares (PLS)	690:716	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	11	used	used	773:776	arg2	LS-SVM					760:765	LS-SVM	760:765	LS-SVM	760:765	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	8	12	theme	hyperspectral	1382:1394	arg1	imaging					1396:1402	hyperspectral imaging	1382:1402	hyperspectral imaging	1382:1402	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	8	12	theme	hyperspectral	1382:1394	arg1	technique					1420:1428	a promising technique	1408:1428	a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1408:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	4	13	theme	standard	659:666	arg1	SNV					684:686	SNV	684:686	SNV	684:686	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	4	13	theme	standard	659:666	arg1	variate					675:681	standard normal variate	659:681	standard normal variate (SNV)	659:687	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	0	14	theme	Multivariate	134:145	arg1	Analysis					147:154	Multivariate Analysis	134:154	Multivariate Analysis	134:154	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	2	15	theme	Hyperspectral	310:322	arg1	imaging					324:330	Hyperspectral imaging	310:330	Hyperspectral imaging as a rapid and nondestructive technique	310:370	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	8	16	theme	rapid	1438:1442	arg1	determination					1457:1469	the rapid and accurate determination	1434:1469	the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1434:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	1	17	theme	active	203:208	arg1	compositions					210:221	active compositions	203:221	active compositions	203:221	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	5	18	theme	prediction	787:796	arg1	models					798:803	prediction models	787:803	prediction models using sample average spectra calculated by preprocessed pixel-wise spectra	787:878	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	4	19	theme	Pixel-wise	565:574	arg1	spectra					576:582	Pixel-wise spectra	565:582	Pixel-wise spectra within all samples	565:601	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	5	20	theme	Partial	690:696	arg1	PLS					713:715	PLS	713:715	PLS	713:715	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	20	theme	Partial	690:696	arg1	squares					704:710	Partial least squares	690:710	Partial least squares (PLS)	690:716	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	1	21	theme	compositions	210:221	arg1	determination					186:198	The rapid and nondestructive determination	157:198	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju)	157:261	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	5	22	theme	pixel-wise	861:870	arg1	spectra					872:878	preprocessed pixel-wise spectra	848:878	preprocessed pixel-wise spectra	848:878	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	8	23	from	morifolium	1510:1519	arg1	determination					1457:1469	the rapid and accurate determination	1434:1469	the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1434:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	5	24	theme	least	698:702	arg1	PLS					713:715	PLS	713:715	PLS	713:715	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	24	theme	least	698:702	arg1	squares					704:710	Partial least squares	690:710	Partial least squares (PLS)	690:716	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	8	25	theme	morifolium	1628:1637	arg1	determination					1597:1609	the quality determination	1585:1609	the quality determination of Chrysanthemum morifolium	1585:1637	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	2	26	theme	nondestructive	347:360	arg1	technique					362:370	a rapid and nondestructive technique	335:370	a rapid and nondestructive technique	335:370	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	7	27	theme	total	1205:1209	arg1	content					1222:1228	total flavonoids content	1205:1228	total flavonoids content in Chrysanthemum morifolium flowers	1205:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	2	28	theme	flavonoids	426:435	arg1	content					437:443	total flavonoids content	420:443	total flavonoids content in Chrysanthemum morifolium	420:471	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	7	29	theme	flavonoids	1211:1220	arg1	content					1222:1228	total flavonoids content	1205:1228	total flavonoids content in Chrysanthemum morifolium flowers	1205:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	7	30	from	maps	1171:1174	arg1	flowers					1258:1264	Chrysanthemum morifolium flowers	1233:1264	Chrysanthemum morifolium flowers	1233:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	6	31	theme	LS-SVM	885:890	arg1	model					892:896	The LS-SVM model	881:896	The LS-SVM model	881:896	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	2	32	used	used	376:379	arg2	imaging					324:330	Hyperspectral imaging	310:330	Hyperspectral imaging as a rapid and nondestructive technique	310:370	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	2	33	theme	total	420:424	arg1	content					437:443	total flavonoids content	420:443	total flavonoids content in Chrysanthemum morifolium	420:471	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	0	34	theme	Polysaccharides	23:37	arg1	Determination					0:12	Determination	0:12	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium	0:86	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	8	35	theme	Chrysanthemum	1614:1626	arg1	morifolium					1628:1637	Chrysanthemum morifolium	1614:1637	Chrysanthemum morifolium	1614:1637	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	8	36	theme	overall	1354:1360	arg1	results					1362:1368	The overall results	1350:1368	The overall results	1350:1368	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	2	37	from	polysaccharides	400:414	arg1	morifolium					462:471	Chrysanthemum morifolium	448:471	Chrysanthemum morifolium	448:471	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	2	38	theme	rapid	337:341	arg1	technique					362:370	a rapid and nondestructive technique	335:370	a rapid and nondestructive technique	335:370	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	8	39	theme	online	1567:1572	arg1	system					1574:1579	an online system	1564:1579	an online system for the quality determination of Chrysanthemum morifolium	1564:1637	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	3	40	theme	morifolium	531:540	arg1	flowers					542:548	Chrysanthemum morifolium flowers	517:548	Chrysanthemum morifolium flowers	517:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	0	41	theme	Total	17:21	arg1	Polysaccharides					23:37	Total Polysaccharides	17:37	Total Polysaccharides	17:37	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	6	42	theme	prediction	1003:1012	arg1	coefficient					966:976	the coefficient	962:976	the coefficient of calibration (R²c) and prediction (R²p)	962:1018	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	8	43	theme	promising	1410:1418	arg1	imaging					1396:1402	hyperspectral imaging	1382:1402	hyperspectral imaging	1382:1402	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	8	43	theme	promising	1410:1418	arg1	technique					1420:1428	a promising technique	1408:1428	a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1408:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	7	44	from	content	1222:1228	arg1	flowers					1258:1264	Chrysanthemum morifolium flowers	1233:1264	Chrysanthemum morifolium flowers	1233:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	7	45	theme	LS-SVM	1296:1301	arg1	models					1303:1308	LS-SVM models	1296:1308	LS-SVM models	1296:1308	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	6	46	theme	coefficient	966:976	arg1	determination					945:957	the determination	941:957	the determination of the coefficient of calibration (R²c) and prediction (R²p)	941:1018	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	3	47	theme	flowers	542:548	arg1	sizes					508:512	different sizes	498:512	different sizes of Chrysanthemum morifolium flowers	498:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	0	48	theme	Total	43:47	arg1	Flavonoids					49:58	Total Flavonoids	43:58	Total Flavonoids	43:58	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	8	49	from	determination	1457:1469	arg1	morifolium					1510:1519	Chrysanthemum morifolium	1496:1519	Chrysanthemum morifolium	1496:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	6	50	theme	PLS	924:926	arg1	models					928:933	the PLS models	920:933	the PLS models	920:933	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	8	51	theme	accurate	1448:1455	arg1	determination					1457:1469	the rapid and accurate determination	1434:1469	the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1434:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	1	52	theme	Chrysanthemum	226:238	arg1	Hangbaiju					252:260	Hangbaiju	252:260	Hangbaiju	252:260	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	1	52	theme	Chrysanthemum	226:238	arg1	morifolium					240:249	Chrysanthemum morifolium	226:249	Chrysanthemum morifolium (Hangbaiju)	226:261	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	8	53	from	ingredients	1481:1491	arg1	morifolium					1510:1519	Chrysanthemum morifolium	1496:1519	Chrysanthemum morifolium	1496:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	3	54	theme	Hyperspectral	474:486	arg1	images					488:493	Hyperspectral images	474:493	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers	474:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	7	55	theme	total	1179:1183	arg1	polysaccharides					1185:1199	total polysaccharides	1179:1199	total polysaccharides	1179:1199	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	7	56	theme	polysaccharides	1185:1199	arg1	content					1222:1228	total flavonoids content	1205:1228	total flavonoids content in Chrysanthemum morifolium flowers	1205:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	7	56	theme	polysaccharides	1185:1199	arg1	maps					1171:1174	Prediction maps	1160:1174	Prediction maps of total polysaccharides	1160:1199	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	2	57	theme	Chrysanthemum	448:460	arg1	morifolium					462:471	Chrysanthemum morifolium	448:471	Chrysanthemum morifolium	448:471	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	7	58	theme	Prediction	1160:1169	arg1	maps					1171:1174	Prediction maps	1160:1174	Prediction maps of total polysaccharides	1160:1199	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	5	59	theme	preprocessed	848:859	arg1	spectra					872:878	preprocessed pixel-wise spectra	848:878	preprocessed pixel-wise spectra	848:878	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	3	60	theme	different	498:506	arg1	sizes					508:512	different sizes	498:512	different sizes of Chrysanthemum morifolium flowers	498:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	0	61	theme	Flavonoids	49:58	arg1	Determination					0:12	Determination	0:12	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium	0:86	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	5	62	theme	squares-support	728:742	arg1	LS-SVM					760:765	LS-SVM	760:765	LS-SVM	760:765	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	62	theme	squares-support	728:742	arg1	machine					751:757	least squares-support vector machine	722:757	least squares-support vector machine (LS-SVM)	722:766	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	7	63	theme	best	1331:1334	arg1	performances					1336:1347	the best performances	1327:1347	the best performances	1327:1347	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
30235811	6	64	theme	calibration	981:991	arg1	coefficient					966:976	the coefficient	962:976	the coefficient of calibration (R²c) and prediction (R²p)	962:1018	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	5	65	theme	vector	744:749	arg1	LS-SVM					760:765	LS-SVM	760:765	LS-SVM	760:765	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	5	65	theme	vector	744:749	arg1	machine					751:757	least squares-support vector machine	722:757	least squares-support vector machine (LS-SVM)	722:766	Partial least squares (PLS) and least squares-support vector machine (LS-SVM) were used to build prediction models using sample average spectra calculated by preprocessed pixel-wise spectra.					
30235811	8	66	theme	great	1537:1541	arg1	potential					1543:1551	the great potential	1533:1551	the great potential to develop an online system for the quality determination of Chrysanthemum morifolium	1533:1637	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	8	67	theme	ingredients	1481:1491	arg1	determination					1457:1469	the rapid and accurate determination	1434:1469	the rapid and accurate determination of active ingredients in Chrysanthemum morifolium	1434:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	2	68	from	content	437:443	arg1	morifolium					462:471	Chrysanthemum morifolium	448:471	Chrysanthemum morifolium	448:471	Hyperspectral imaging as a rapid and nondestructive technique was used to determine total polysaccharides and total flavonoids content in Chrysanthemum morifolium.					
30235811	6	69	theme	content	1140:1146	arg1	prediction					1148:1157	total flavonoids content prediction	1123:1157	total flavonoids content prediction	1123:1157	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	6	70	theme	flavonoids	1129:1138	arg1	prediction					1148:1157	total flavonoids content prediction	1123:1157	total flavonoids content prediction	1123:1157	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	6	71	theme	predictive	1053:1062	arg1	RPD					1075:1077	RPD	1075:1077	RPD	1075:1077	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	6	71	theme	predictive	1053:1062	arg1	deviation					1064:1072	the residual predictive deviation	1040:1072	the residual predictive deviation (RPD)	1040:1078	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	8	72	theme	active	1474:1479	arg1	ingredients					1481:1491	active ingredients	1474:1491	active ingredients in Chrysanthemum morifolium	1474:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	1	73	theme	rapid	161:165	arg1	determination					186:198	The rapid and nondestructive determination	157:198	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju)	157:261	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	6	74	theme	residual	1044:1051	arg1	RPD					1075:1077	RPD	1075:1077	RPD	1075:1077	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	6	74	theme	residual	1044:1051	arg1	deviation					1064:1072	the residual predictive deviation	1040:1072	the residual predictive deviation (RPD)	1040:1078	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	3	75	theme	Chrysanthemum	517:529	arg1	flowers					542:548	Chrysanthemum morifolium flowers	517:548	Chrysanthemum morifolium flowers	517:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	8	76	theme	Chrysanthemum	1496:1508	arg1	morifolium					1510:1519	Chrysanthemum morifolium	1496:1519	Chrysanthemum morifolium	1496:1519	The overall results showed that hyperspectral imaging was a promising technique for the rapid and accurate determination of active ingredients in Chrysanthemum morifolium, indicating the great potential to develop an online system for the quality determination of Chrysanthemum morifolium.					
30235811	6	77	theme	total	1123:1127	arg1	prediction					1148:1157	total flavonoids content prediction	1123:1157	total flavonoids content prediction	1123:1157	The LS-SVM model performed better than the PLS models, with the determination of the coefficient of calibration (R²c) and prediction (R²p) being over 0.90 and the residual predictive deviation (RPD) being over 3 for total polysaccharides and total flavonoids content prediction.					
30235811	3	78	theme	sizes	508:512	arg1	images					488:493	Hyperspectral images	474:493	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers	474:548	Hyperspectral images of different sizes of Chrysanthemum morifolium flowers were acquired.					
30235811	1	79	theme	great	269:273	arg1	value					275:279	great value	269:279	great value for producers and consumers	269:307	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	0	80	theme	Chrysanthemum	63:75	arg1	morifolium					77:86	Chrysanthemum morifolium	63:86	Chrysanthemum morifolium	63:86	Determination of Total Polysaccharides and Total Flavonoids in Chrysanthemum morifolium Using Near-Infrared Hyperspectral Imaging and Multivariate Analysis.					
30235811	4	81	dep	wavelet	624:630	arg1	WT					643:644	WT	643:644	WT	643:644	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	4	81	dep	wavelet	624:630	arg1	transform					632:640	transform	632:640	transform	632:640	Pixel-wise spectra within all samples were preprocessed by wavelet transform (WT) followed by standard normal variate (SNV).					
30235811	1	82	theme	nondestructive	171:184	arg1	determination					186:198	The rapid and nondestructive determination	157:198	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju)	157:261	The rapid and nondestructive determination of active compositions in Chrysanthemum morifolium (Hangbaiju) is of great value for producers and consumers.					
30235811	7	83	theme	morifolium	1247:1256	arg1	flowers					1258:1264	Chrysanthemum morifolium flowers	1233:1264	Chrysanthemum morifolium flowers	1233:1264	Prediction maps of total polysaccharides and total flavonoids content in Chrysanthemum morifolium flowers were successfully obtained by LS-SVM models, which exhibited the best performances.					
29385683	2	0	theme	acidic	371:376	arg1	heteropolysaccharide					378:397	an acidic heteropolysaccharide	368:397	an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage)	368:488	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	2	0	theme	acidic	371:376	arg1	TPPPS					358:362	TPPPS	358:362	TPPPS	358:362	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	3	1	theme	TPPPS	578:582	arg1	spectra					567:573	the spectra	563:573	the spectra of TPPPS	563:582	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	7	2	theme	acute	1491:1495	arg1	hepatotoxicity					1497:1510	acute hepatotoxicity	1491:1510	acute hepatotoxicity induced by CCl₄	1491:1526	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	7	3	theme	antioxidant	1426:1436	arg1	activities					1438:1447	strong antioxidant activities	1419:1447	strong antioxidant activities	1419:1447	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	4	4	theme	dose-dependent	711:724	arg1	activities					738:747	dose-dependent antioxidant activities	711:747	dose-dependent antioxidant activities	711:747	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	1	5	theme	chemical	192:199	arg1	composition					201:211	the chemical composition	188:211	the chemical composition	188:211	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	4	6	theme	activities	738:747	arg1	degrees					700:706	different degrees	690:706	different degrees of dose-dependent antioxidant activities	690:747	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	6	7	theme	liver	1324:1328	arg1	tissues					1330:1336	the liver tissues	1320:1336	the liver tissues	1320:1336	Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation.					
29385683	5	8	theme	lactic	1069:1074	arg1	dehydrogenase					1076:1088	lactic dehydrogenase	1069:1088	lactic dehydrogenase (LDH)	1069:1094	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	8	theme	lactic	1069:1074	arg1	LDH					1091:1093	LDH	1091:1093	LDH	1091:1093	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	3	9	theme	infrared	594:601	arg1	peaks					614:618	infrared absorption peaks	594:618	infrared absorption peaks characteristic of polysaccharides	594:652	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	4	10	with	stress	822:827	arg1	doses					860:864	three tested doses	847:864	three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	847:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	1	11	theme	hepatoprotective	239:254	arg1	effect					256:261	hepatoprotective effect	239:261	hepatoprotective effect	239:261	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	4	12	from	TPPPS	869:873	arg1	rats					907:910	rats	907:910	rats	907:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	4	13	dep	in	658:659	arg1	vitro					661:665	vitro	661:665	vitro	661:665	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	1	14	from	composition	201:211	arg1	TPPPS					324:328	TPPPS	324:328	TPPPS	324:328	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	14	from	composition	201:211	arg1	massoniana					305:314	massoniana	305:314	massoniana	305:314	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	15	theme	present	153:159	arg1	study					161:165	the present study	149:165	the present study	149:165	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	0	16	theme	Polysaccharides	77:91	arg1	Antioxidant					30:40	Antioxidant	30:40	Antioxidant	30:40	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	16	theme	Polysaccharides	77:91	arg1	Activities					63:72	Hepatoprotective Activities	46:72	Hepatoprotective Activities	46:72	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	16	theme	Polysaccharides	77:91	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	5	17	with	Pretreatment	913:924	arg1	TPPPS					931:935	TPPPS	931:935	TPPPS	931:935	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	0	18	theme	Taishan	98:104	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	8	19	theme	hepatoprotective	1533:1548	arg1	effect					1550:1555	The hepatoprotective effect	1529:1555	The hepatoprotective effect	1529:1555	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	8	19	theme	hepatoprotective	1533:1548	arg1	related					1571:1577	related	1571:1577	related	1571:1577	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	0	20	theme	massoniana	112:121	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	21	from	Activities	63:72	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	5	22	theme	malondialdehyde	1100:1114	arg1	levels					965:970	the levels	961:970	the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries	961:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	23	from	stress	822:827	arg1	liver					836:840	the liver	832:840	the liver	832:840	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	2	24	theme	molar	472:476	arg1	%					469:469	79.6%	465:469	79.6%	465:469	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	2	24	theme	molar	472:476	arg1	percentage					478:487	molar percentage	472:487	molar percentage	472:487	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	5	25	theme	superoxide	1176:1185	arg1	SOD					1198:1200	SOD	1198:1200	SOD	1198:1200	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	25	theme	superoxide	1176:1185	arg1	dismutase					1187:1195	superoxide dismutase	1176:1195	superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px)	1176:1244	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	26	theme	CCl₄-induced	799:810	arg1	stress					822:827	CCl₄-induced oxidative stress	799:827	CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	799:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	2	27	theme	arabinose	416:424	arg1	monosaccharides					448:462	glucose and arabinose as the main component monosaccharides	404:462	glucose and arabinose as the main component monosaccharides (79.6%, molar percentage)	404:488	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	0	28	theme	Preliminary	0:10	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	4	29	theme	stress	822:827	arg1	suppression					784:794	suppression	784:794	suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	784:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	5	30	theme	aminotransferase	983:998	arg1	levels					965:970	the levels	961:970	the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries	961:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	8	31	theme	free	1586:1589	arg1	scavenging					1599:1608	its free radical scavenging	1582:1608	its free radical scavenging effect	1582:1615	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	8	32	theme	scavenging	1599:1608	arg1	effect					1610:1615	its free radical scavenging effect	1582:1615	its free radical scavenging effect	1582:1615	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	2	33	with	heteropolysaccharide	378:397	arg1	monosaccharides					448:462	glucose and arabinose as the main component monosaccharides	404:462	glucose and arabinose as the main component monosaccharides (79.6%, molar percentage)	404:488	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	5	34	theme	phosphatase	1050:1060	arg1	levels					965:970	the levels	961:970	the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries	961:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	35	theme	tested	853:858	arg1	doses					860:864	three tested doses	847:864	three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	847:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	3	36	theme	transform-infrared	499:516	arg1	analysis					539:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	5	37	theme	aminotransferase	1017:1032	arg1	levels					965:970	the levels	961:970	the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries	961:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	8	38	theme	antioxidant	1629:1639	arg1	activity					1641:1648	antioxidant activity	1629:1648	antioxidant activity	1629:1648	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	2	39	theme	glucose	404:410	arg1	monosaccharides					448:462	glucose and arabinose as the main component monosaccharides	404:462	glucose and arabinose as the main component monosaccharides (79.6%, molar percentage)	404:488	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	5	40	theme	dismutase	1187:1195	arg1	activities					1162:1171	the activities	1158:1171	the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px)	1158:1244	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	41	theme	mg/kg	894:898	arg1	bw					900:901	100, 200, and 400 mg/kg bw	876:901	100, 200, and 400 mg/kg bw	876:901	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	4	41	theme	mg/kg	894:898	arg1	TPPPS					869:873	TPPPS	869:873	TPPPS (100, 200, and 400 mg/kg bw) in rats	869:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	8	42	theme	lipid	1665:1669	arg1	peroxidation					1671:1682	lipid peroxidation	1665:1682	lipid peroxidation	1665:1682	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	6	43	theme	Histopathological	1247:1263	arg1	observation					1265:1275	Histopathological observation	1247:1275	Histopathological observation	1247:1275	Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation.					
29385683	7	44	theme	significant	1453:1463	arg1	effect					1476:1481	significant protective effect	1453:1481	significant protective effect	1453:1481	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	7	45	theme	protective	1465:1474	arg1	effect					1476:1481	significant protective effect	1453:1481	significant protective effect	1453:1481	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	3	46	theme	absorption	603:612	arg1	peaks					614:618	infrared absorption peaks	594:618	infrared absorption peaks characteristic of polysaccharides	594:652	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	7	47	theme	strong	1419:1424	arg1	activities					1438:1447	strong antioxidant activities	1419:1447	strong antioxidant activities	1419:1447	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	4	48	theme	antioxidant	726:736	arg1	activities					738:747	dose-dependent antioxidant activities	711:747	dose-dependent antioxidant activities	711:747	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	2	49	theme	HPLC	332:335	arg1	analysis					337:344	HPLC analysis	332:344	HPLC analysis	332:344	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	1	50	theme	antioxidant	214:224	arg1	activity					226:233	antioxidant activity	214:233	antioxidant activity	214:233	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	5	51	theme	dehydrogenase	1076:1088	arg1	levels					965:970	the levels	961:970	the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries	961:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	3	52	theme	polysaccharides	638:652	arg1	characteristic					620:633	characteristic	620:633	characteristic	620:633	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	4	53	theme	different	690:698	arg1	degrees					700:706	different degrees	690:706	different degrees of dose-dependent antioxidant activities	690:747	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	3	54	theme	characteristic	620:633	arg1	peaks					614:618	infrared absorption peaks	594:618	infrared absorption peaks characteristic of polysaccharides	594:652	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	1	55	from	effect	256:261	arg1	TPPPS					324:328	TPPPS	324:328	TPPPS	324:328	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	55	from	effect	256:261	arg1	massoniana					305:314	massoniana	305:314	massoniana	305:314	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	56	from	activity	226:233	arg1	TPPPS					324:328	TPPPS	324:328	TPPPS	324:328	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	56	from	activity	226:233	arg1	massoniana					305:314	massoniana	305:314	massoniana	305:314	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	0	57	from	Pollen	123:128	arg1	Polysaccharides					77:91	Polysaccharides	77:91	Polysaccharides from Taishan Pinus massoniana Pollen	77:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	57	from	Pollen	123:128	arg1	Antioxidant					30:40	Antioxidant	30:40	Antioxidant	30:40	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	57	from	Pollen	123:128	arg1	Activities					63:72	Hepatoprotective Activities	46:72	Hepatoprotective Activities	46:72	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	0	57	from	Pollen	123:128	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization	0:27	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	4	58	from	rats	907:910	arg1	doses					860:864	three tested doses	847:864	three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	847:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	4	59	from	doses	860:864	arg1	rats					907:910	rats	907:910	rats	907:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	1	60	theme	study	161:165	arg1	objectives					135:144	The objectives	131:144	The objectives of the present study	131:165	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	0	61	from	Antioxidant	30:40	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	5	62	theme	peroxidase	1226:1235	arg1	activities					1162:1171	the activities	1158:1171	the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px)	1158:1244	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	6	63	theme	histological	1356:1367	arg1	alternation					1369:1379	CCl₄-induced histological alternation	1343:1379	CCl₄-induced histological alternation	1343:1379	Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation.					
29385683	0	64	theme	Pinus	106:110	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	1	65	from	massoniana	305:314	arg1	composition					201:211	the chemical composition	188:211	the chemical composition	188:211	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	65	from	massoniana	305:314	arg1	activity					226:233	antioxidant activity	214:233	antioxidant activity	214:233	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	65	from	massoniana	305:314	arg1	effect					256:261	hepatoprotective effect	239:261	hepatoprotective effect	239:261	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	65	from	massoniana	305:314	arg1	polysaccharides					270:284	the polysaccharides	266:284	the polysaccharides from Taishan Pinus massoniana pollen (TPPPS)	266:329	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	0	66	from	Characterization	12:27	arg1	Pollen					123:128	Taishan Pinus massoniana Pollen	98:128	Taishan Pinus massoniana Pollen	98:128	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	5	67	theme	CCl₄	1130:1133	arg1	injuries					1135:1142	CCl₄ injuries	1130:1142	CCl₄ injuries	1130:1142	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	68	from	liver	836:840	arg1	suppression					784:794	suppression	784:794	suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	784:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	6	69	theme	CCl₄-induced	1343:1354	arg1	alternation					1369:1379	CCl₄-induced histological alternation	1343:1379	CCl₄-induced histological alternation	1343:1379	Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation.					
29385683	5	70	theme	alanine	975:981	arg1	AST					1001:1003	AST	1001:1003	AST	1001:1003	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	70	theme	alanine	975:981	arg1	aminotransferase					983:998	alanine aminotransferase	975:998	alanine aminotransferase (AST)	975:1004	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	8	71	theme	radical	1591:1597	arg1	scavenging					1599:1608	its free radical scavenging	1582:1608	its free radical scavenging effect	1582:1615	The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.					
29385683	4	72	theme	oxidative	812:820	arg1	stress					822:827	CCl₄-induced oxidative stress	799:827	CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	799:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	3	73	theme	Fourier	491:497	arg1	analysis					539:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	0	74	theme	Hepatoprotective	46:61	arg1	Activities					63:72	Hepatoprotective Activities	46:72	Hepatoprotective Activities	46:72	Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.					
29385683	7	75	contain	has	1415:1417	arg2	activities					1438:1447	strong antioxidant activities	1419:1447	strong antioxidant activities	1419:1447	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	7	75	contain	has	1415:1417	arg1	TPPPS					1409:1413	TPPPS	1409:1413	TPPPS	1409:1413	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	7	75	contain	has	1415:1417	arg2	effect					1476:1481	significant protective effect	1453:1481	significant protective effect	1453:1481	These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄.					
29385683	4	76	theme	TPPPS	869:873	arg1	doses					860:864	three tested doses	847:864	three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats	847:910	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	2	77	theme	main	433:436	arg1	component					438:446	the main component	429:446	the main component	429:446	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	5	78	theme	alkaline	1041:1048	arg1	ALP					1063:1065	ALP	1063:1065	ALP	1063:1065	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	78	theme	alkaline	1041:1048	arg1	phosphatase					1050:1060	alkaline phosphatase	1041:1060	alkaline phosphatase (ALP)	1041:1066	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	2	79	dep	monosaccharides	448:462	arg1	%					469:469	79.6%	465:469	79.6%	465:469	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	2	79	dep	monosaccharides	448:462	arg1	percentage					478:487	molar percentage	472:487	molar percentage	472:487	HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage).					
29385683	3	80	theme	FT-IR	532:536	arg1	analysis					539:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	4	81	from	suppression	784:794	arg1	liver					836:840	the liver	832:840	the liver	832:840	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	5	82	theme	aspartate	1007:1015	arg1	ALT					1035:1037	ALT	1035:1037	ALT	1035:1037	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	82	theme	aspartate	1007:1015	arg1	aminotransferase					1017:1032	aspartate aminotransferase	1007:1032	aspartate aminotransferase (ALT)	1007:1038	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	4	83	theme	in	658:659	arg1	assays					667:672	in vitro assays	658:672	in vitro assays	658:672	In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats.					
29385683	3	84	theme	spectroscopy	518:529	arg1	analysis					539:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis	491:546	Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides.					
29385683	1	85	theme	polysaccharides	270:284	arg1	composition					201:211	the chemical composition	188:211	the chemical composition	188:211	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	85	theme	polysaccharides	270:284	arg1	activity					226:233	antioxidant activity	214:233	antioxidant activity	214:233	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	1	85	theme	polysaccharides	270:284	arg1	effect					256:261	hepatoprotective effect	239:261	hepatoprotective effect	239:261	The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan Pinus massoniana pollen (TPPPS).					
29385683	5	86	theme	glutathione	1214:1224	arg1	GSH-Px					1238:1243	GSH-Px	1238:1243	GSH-Px	1238:1243	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
29385683	5	86	theme	glutathione	1214:1224	arg1	peroxidase					1226:1235	glutathione peroxidase	1214:1235	superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px)	1176:1244	Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px).					
31720977	0	0	theme	fermentans	82:91	arg1	JCM9966					93:99	Actinotalea fermentans JCM9966	70:99	Actinotalea fermentans JCM9966	70:99	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	3	1	from	capability	708:717	arg1	hemicelluloses					748:761	degrading hemicelluloses	738:761	degrading hemicelluloses	738:761	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	1	2	theme	hemicellulose-degrading	151:173	arg1	Xylanosomes					102:112	Xylanosomes	102:112	Xylanosomes	102:112	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	1	2	theme	hemicellulose-degrading	151:173	arg1	complexes					198:206	hemicellulose-degrading nano-scale multienzyme complexes	151:206	hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi	151:261	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	0	3	theme	Actinotalea	70:80	arg1	JCM9966					93:99	Actinotalea fermentans JCM9966	70:99	Actinotalea fermentans JCM9966	70:99	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	1	4	theme	nano-scale	175:184	arg1	Xylanosomes					102:112	Xylanosomes	102:112	Xylanosomes	102:112	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	1	4	theme	nano-scale	175:184	arg1	complexes					198:206	hemicellulose-degrading nano-scale multienzyme complexes	151:206	hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi	151:261	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	2	5	theme	MECs	300:303	arg1	structure					394:402	subunit structure	386:402	subunit structure	386:402	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	2	5	theme	MECs	300:303	arg1	isolation					283:291	the isolation	279:291	the isolation of the MECs produced by Actinotalea fermentas JCM9966	279:345	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	2	5	theme	MECs	300:303	arg1	studies					374:380	the functional studies	359:380	the functional studies	359:380	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	3	6	theme	conserved	558:566	arg1	proteins					581:588	several conserved multi-domain proteins	550:588	several conserved multi-domain proteins	550:588	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	1	7	theme	multienzyme	186:196	arg1	Xylanosomes					102:112	Xylanosomes	102:112	Xylanosomes	102:112	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	1	7	theme	multienzyme	186:196	arg1	complexes					198:206	hemicellulose-degrading nano-scale multienzyme complexes	151:206	hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi	151:261	Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi.					
31720977	3	8	theme	degrading	738:746	arg1	hemicelluloses					748:761	degrading hemicelluloses	738:761	degrading hemicelluloses	738:761	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	2	9	theme	subunit	386:392	arg1	structure					394:402	subunit structure	386:402	subunit structure	386:402	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	2	10	theme	Actinotalea	317:327	arg1	fermentas					329:337	Actinotalea fermentas JCM9966	317:345	Actinotalea fermentas JCM9966	317:345	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	3	11	theme	isolated	447:454	arg1	MECs					456:459	The isolated MECs	443:459	The isolated MECs	443:459	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	12	theme	multi-domain	568:579	arg1	proteins					581:588	several conserved multi-domain proteins	550:588	several conserved multi-domain proteins	550:588	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	13	theme	particle	480:487	arg1	size					489:492	similar particle size	472:492	similar particle size	472:492	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	0	14	theme	subunit	14:20	arg1	structure					22:30	subunit structure	14:30	subunit structure	14:30	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	3	15	dep	F16	540:542	arg1	have					545:548	have	545:548	have	545:548	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	2	16	theme	proteomic	416:424	arg1	identifications					426:440	proteomic identifications	416:440	proteomic identifications	416:440	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
31720977	3	17	theme	weight	660:665	arg1	compositions					675:686	low molecular weight subunit compositions	646:686	low molecular weight subunit compositions	646:686	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	18	theme	enzymatic	621:629	arg1	activities					631:640	enzymatic activities	621:640	enzymatic activities	621:640	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	19	theme	similar	472:478	arg1	size					489:492	similar particle size	472:492	similar particle size	472:492	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	0	20	theme	complex	50:56	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	0	20	theme	complex	50:56	arg1	structure					22:30	subunit structure	14:30	subunit structure	14:30	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	3	21	theme	subunit	667:673	arg1	compositions					675:686	low molecular weight subunit compositions	646:686	low molecular weight subunit compositions	646:686	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	0	22	theme	xylanosome	39:48	arg1	complex					50:56	the xylanosome complex	35:56	the xylanosome complex	35:56	Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.					
31720977	3	23	theme	low	646:648	arg1	compositions					675:686	low molecular weight subunit compositions	646:686	low molecular weight subunit compositions	646:686	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	24	theme	molecular	650:658	arg1	compositions					675:686	low molecular weight subunit compositions	646:686	low molecular weight subunit compositions	646:686	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	25	theme	diverse	700:706	arg1	capability					708:717	diverse capability	700:717	diverse capability as well as bias in degrading hemicelluloses	700:761	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	26	from	bias	730:733	arg1	hemicelluloses					748:761	degrading hemicelluloses	738:761	degrading hemicelluloses	738:761	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	3	27	theme	several	550:556	arg1	proteins					581:588	several conserved multi-domain proteins	550:588	several conserved multi-domain proteins	550:588	The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.					
31720977	2	28	theme	functional	363:372	arg1	studies					374:380	the functional studies	359:380	the functional studies	359:380	Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications.					
30116038	7	0	theme	alginate/pectin-specific	1289:1312	arg1	secretion					1314:1322	the alginate/pectin-specific secretion	1285:1322	the alginate/pectin-specific secretion of pyrroloquinoline quinone	1285:1350	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	2	1	from	abundant	357:364	arg1	habitats					377:384	natural habitats	369:384	natural habitats	369:384	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	3	2	theme	algal	593:597	arg1	polysaccharides					599:613	multiple algal polysaccharides	584:613	multiple algal polysaccharides	584:613	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	9	3	theme	CAZyme	1680:1685	arg1	microdiversity					1687:1700	CAZyme microdiversity	1680:1700	CAZyme microdiversity	1680:1700	Moreover, CAZyme microdiversity and the genomic predisposition towards polysaccharide mixtures among Alteromonas spp.					
30116038	8	4	theme	ecological	1456:1465	arg1	relevance					1467:1475	The ecological relevance	1452:1475	The ecological relevance of cellular adaptations	1452:1499	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	11	5	theme	substantial	1977:1987	arg1	productivity					1997:2008	the substantial primary productivity	1973:2008	the substantial primary productivity of algae on global scales	1973:2034	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	2	6	theme	bacterial	293:301	arg1	degradation					303:313	the bacterial degradation	289:313	the bacterial degradation of polysaccharide mixtures	289:340	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	1	7	theme	central	198:204	arg1	component					206:214	central component	198:214	central component of marine food webs	198:234	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	10	8	theme	utilization	1904:1914	arg1	types					1916:1920	carbohydrate utilization types	1891:1920	distinct 'carbohydrate utilization types' with different ecological strategies	1881:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	5	9	theme	alginate/pectin	978:992	arg1	utilization					994:1004	simultaneous alginate/pectin utilization	965:1004	simultaneous alginate/pectin utilization	965:1004	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	8	10	theme	adaptations	1489:1499	arg1	relevance					1467:1475	The ecological relevance	1452:1475	The ecological relevance of cellular adaptations	1452:1499	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	4	11	theme	laminarin	806:814	arg1	mix					799:801	a mix	797:801	a mix of laminarin, alginate and pectin	797:835	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	7	12	theme	temporal	1228:1235	arg1	changes					1237:1243	Distinct temporal changes	1219:1243	Distinct temporal changes	1219:1243	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	7	12	theme	temporal	1228:1235	arg1	secretion					1314:1322	the alginate/pectin-specific secretion	1285:1322	the alginate/pectin-specific secretion of pyrroloquinoline quinone	1285:1350	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	2	13	theme	mixtures	333:340	arg1	degradation					303:313	the bacterial degradation	289:313	the bacterial degradation of polysaccharide mixtures	289:340	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	5	14	theme	initial	923:929	arg1	utilization					941:951	initial laminarin utilization	923:951	initial laminarin utilization followed by simultaneous alginate/pectin utilization	923:1004	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	10	15	theme	different	1928:1936	arg1	strategies					1949:1958	different ecological strategies	1928:1958	different ecological strategies	1928:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	2	16	theme	cellular	246:253	arg1	aspects					270:276	cellular and ecological aspects	246:276	cellular and ecological aspects	246:276	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	6	17	theme	gene	1067:1070	arg1	expression					1072:1081	gene expression	1067:1081	gene expression	1067:1081	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	8	18	theme	molecular	1519:1527	arg1	evidence					1529:1536	molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan	1519:1667	molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan	1519:1667	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	11	19	theme	polysaccharide	2162:2175	arg1	pools					2177:2181	chemically diverse polysaccharide pools	2143:2181	chemically diverse polysaccharide pools	2143:2181	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	19	theme	polysaccharide	2162:2175	arg1	step					2190:2193	a key step	2184:2193	a key step in marine carbon cycling	2184:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	9	20	theme	Alteromonas	1771:1781	arg1	spp					1783:1785	Alteromonas spp	1771:1785	Alteromonas spp	1771:1785	Moreover, CAZyme microdiversity and the genomic predisposition towards polysaccharide mixtures among Alteromonas spp.					
30116038	3	21	theme	multiple	584:591	arg1	polysaccharides					599:613	multiple algal polysaccharides	584:613	multiple algal polysaccharides	584:613	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	6	22	theme	utilization	1168:1178	arg1	loci					1180:1183	CAZymes/polysaccharide utilization loci	1145:1183	CAZymes/polysaccharide utilization loci but also other functional traits	1145:1216	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	0	23	theme	macleodii	73:81	arg1	adaptations					18:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	0	23	theme	macleodii	73:81	arg1	implications					45:56	ecological implications	34:56	ecological implications	34:56	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	5	24	theme	substrate	860:868	arg1	prioritization					870:883	substrate prioritization	860:883	substrate prioritization driven by catabolite repression	860:915	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	1	25	theme	bacterial	168:176	arg1	polysaccharides					135:149	Algal polysaccharides	129:149	Algal polysaccharides	129:149	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	25	theme	bacterial	168:176	arg1	source					187:192	an important bacterial nutrient source	155:192	an important bacterial nutrient source	155:192	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	4	26	theme	cellular	740:747	arg1	adaptations					749:759	cellular adaptations	740:759	cellular adaptations of A. macleodii 83-1	740:780	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	11	27	theme	bacteria-algae	2087:2100	arg1	interactions					2102:2113	bacteria-algae interactions	2087:2113	bacteria-algae interactions	2087:2113	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	6	28	theme	metabolite	1106:1115	arg1	secretion					1117:1125	metabolite secretion	1106:1125	metabolite secretion	1106:1125	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	3	29	theme	bacterial	474:482	arg1	utilization					484:494	their bacterial utilization	468:494	their bacterial utilization	468:494	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	0	30	theme	polysaccharides	112:126	arg1	mixture					95:101	a mixture	93:101	a mixture of algal polysaccharides	93:126	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	4	31	dep	A.	764:765	arg1	macleodii					767:775	macleodii	767:775	macleodii	767:775	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	11	32	from	step	2190:2193	arg1	cycling					2212:2218	marine carbon cycling	2198:2218	marine carbon cycling	2198:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	0	33	theme	Biphasic	0:7	arg1	adaptations					18:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	8	34	from	marine macroalgae	1550:1566	arg1	Fucus					1598:1602	Fucus	1598:1602	Fucus	1598:1602	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	8	34	from	marine macroalgae	1550:1566	arg1	Saccharina					1583:1592	particular Saccharina	1572:1592	particular Saccharina	1572:1592	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	11	35	from	productivity	1997:2008	arg1	scales					2029:2034	global scales	2022:2034	global scales	2022:2034	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	3	36	from	degradation	649:659	arg1	oceans					668:673	the oceans	664:673	the oceans	664:673	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	6	37	theme	other	1194:1198	arg1	traits					1211:1216	other functional traits	1194:1216	CAZymes/polysaccharide utilization loci but also other functional traits	1145:1216	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	3	38	theme	polysaccharide	440:453	arg1	mixtures					455:462	marine polysaccharide mixtures	433:462	marine polysaccharide mixtures	433:462	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	7	39	theme	substrate-dependent	1366:1384	arg1	adaptations					1386:1396	substrate-dependent adaptations	1366:1396	substrate-dependent adaptations	1366:1396	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	7	40	theme	quinone	1344:1350	arg1	secretion					1314:1322	the alginate/pectin-specific secretion	1285:1322	the alginate/pectin-specific secretion of pyrroloquinoline quinone	1285:1350	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	0	41	theme	ecological	34:43	arg1	implications					45:56	ecological implications	34:56	ecological implications	34:56	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	8	42	theme	rhamnogalacturonan	1650:1667	arg1	mixtures					1613:1620	mixtures	1613:1620	mixtures of alginate and pectin-like rhamnogalacturonan	1613:1667	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	3	43	theme	bacterium	528:536	arg1	83-1					560:563	the model bacterium Alteromonas macleodii 83-1	518:563	the model bacterium Alteromonas macleodii 83-1	518:563	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	10	44	with	types	1916:1920	arg1	strategies					1949:1958	different ecological strategies	1928:1958	different ecological strategies	1928:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	2	45	theme	ecological	259:268	arg1	aspects					270:276	cellular and ecological aspects	246:276	cellular and ecological aspects	246:276	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	10	46	theme	polysaccharide-related	1796:1817	arg1	factor					1849:1854	an ecophysiological factor	1829:1854	an ecophysiological factor	1829:1854	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	10	46	theme	polysaccharide-related	1796:1817	arg1	traits					1819:1824	polysaccharide-related traits	1796:1824	polysaccharide-related traits	1796:1824	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	11	47	theme	carbon	2205:2210	arg1	cycling					2212:2218	marine carbon cycling	2198:2218	marine carbon cycling	2198:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	48	theme	key	2186:2188	arg1	pools					2177:2181	chemically diverse polysaccharide pools	2143:2181	chemically diverse polysaccharide pools	2143:2181	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	48	theme	key	2186:2188	arg1	step					2190:2193	a key step	2184:2193	a key step in marine carbon cycling	2184:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	4	49	theme	A.	764:765	arg1	83-1					777:780	A. macleodii 83-1	764:780	A. macleodii 83-1	764:780	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	6	50	theme	pronounced	1046:1055	arg1	shifts					1057:1062	pronounced shifts	1046:1062	pronounced shifts	1046:1062	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	4	51	theme	Transcriptomic	676:689	arg1	profiling					721:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	10	52	theme	ecophysiological	1832:1847	arg1	factor					1849:1854	an ecophysiological factor	1829:1854	an ecophysiological factor	1829:1854	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	10	52	theme	ecophysiological	1832:1847	arg1	traits					1819:1824	polysaccharide-related traits	1796:1824	polysaccharide-related traits	1796:1824	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	3	53	theme	macleodii	550:558	arg1	83-1					560:563	the model bacterium Alteromonas macleodii 83-1	518:563	the model bacterium Alteromonas macleodii 83-1	518:563	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	1	54	theme	food	226:229	arg1	webs					231:234	marine food webs	219:234	marine food webs	219:234	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	2	55	theme	polysaccharide	318:331	arg1	mixtures					333:340	polysaccharide mixtures	318:340	polysaccharide mixtures	318:340	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	6	56	theme	biphasic	1012:1019	arg1	phenotype					1021:1029	This biphasic phenotype	1007:1029	This biphasic phenotype	1007:1029	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	2	57	from	habitats	377:384	arg1	abundant					357:364	abundant	357:364	abundant	357:364	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	7	58	theme	exometabolome	1248:1260	arg1	composition					1262:1272	exometabolome composition	1248:1272	exometabolome composition	1248:1272	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	11	59	theme	primary	1989:1995	arg1	productivity					1997:2008	the substantial primary productivity	1973:2008	the substantial primary productivity of algae on global scales	1973:2034	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	5	60	theme	simultaneous	965:976	arg1	utilization					994:1004	simultaneous alginate/pectin utilization	965:1004	simultaneous alginate/pectin utilization	965:1004	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	10	61	theme	carbohydrate	1891:1902	arg1	types					1916:1920	carbohydrate utilization types	1891:1920	distinct 'carbohydrate utilization types' with different ecological strategies	1881:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	11	62	theme	diverse	2154:2160	arg1	pools					2177:2181	chemically diverse polysaccharide pools	2143:2181	chemically diverse polysaccharide pools	2143:2181	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	62	theme	diverse	2154:2160	arg1	step					2190:2193	a key step	2184:2193	a key step in marine carbon cycling	2184:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	7	63	theme	Distinct	1219:1226	arg1	changes					1237:1243	Distinct temporal changes	1219:1243	Distinct temporal changes	1219:1243	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	7	63	theme	Distinct	1219:1226	arg1	secretion					1314:1322	the alginate/pectin-specific secretion	1285:1322	the alginate/pectin-specific secretion of pyrroloquinoline quinone	1285:1350	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	1	64	theme	marine	219:224	arg1	webs					231:234	marine food webs	219:234	marine food webs	219:234	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	6	65	theme	protein	1084:1090	arg1	abundance					1092:1100	protein abundance	1084:1100	protein abundance	1084:1100	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	4	66	theme	proteomic	692:700	arg1	profiling					721:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	10	67	theme	ecological	1938:1947	arg1	strategies					1949:1958	different ecological strategies	1928:1958	different ecological strategies	1928:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	2	68	theme	natural	369:375	arg1	habitats					377:384	natural habitats	369:384	natural habitats	369:384	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	9	69	theme	genomic	1710:1716	arg1	predisposition					1718:1731	the genomic predisposition	1706:1731	the genomic predisposition	1706:1731	Moreover, CAZyme microdiversity and the genomic predisposition towards polysaccharide mixtures among Alteromonas spp.					
30116038	5	70	theme	laminarin	931:939	arg1	utilization					941:951	initial laminarin utilization	923:951	initial laminarin utilization followed by simultaneous alginate/pectin utilization	923:1004	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	4	71	theme	exometabolomic	706:719	arg1	profiling					721:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling	676:729	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	7	72	theme	chemical	1408:1415	arg1	interactions					1417:1428	chemical interactions	1408:1428	chemical interactions within the community	1408:1449	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	3	73	theme	polysaccharide	634:647	arg1	degradation					649:659	polysaccharide degradation	634:659	polysaccharide degradation in the oceans	634:673	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	1	74	theme	Algal	129:133	arg1	polysaccharides					135:149	Algal polysaccharides	129:149	Algal polysaccharides	129:149	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	74	theme	Algal	129:133	arg1	source					187:192	an important bacterial nutrient source	155:192	an important bacterial nutrient source	155:192	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	11	75	theme	pools	2177:2181	arg1	understanding					2070:2082	the understanding	2066:2082	the understanding of bacteria-algae interactions	2066:2113	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	75	theme	pools	2177:2181	arg1	remineralization					2123:2138	the remineralization	2119:2138	the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling	2119:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	1	76	theme	important	158:166	arg1	polysaccharides					135:149	Algal polysaccharides	129:149	Algal polysaccharides	129:149	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	76	theme	important	158:166	arg1	source					187:192	an important bacterial nutrient source	155:192	an important bacterial nutrient source	155:192	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	6	77	theme	CAZymes/polysaccharide	1145:1166	arg1	loci					1180:1183	CAZymes/polysaccharide utilization loci	1145:1183	CAZymes/polysaccharide utilization loci but also other functional traits	1145:1216	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	8	78	theme	common	1543:1548	arg1	marine macroalgae					1550:1566	common marine macroalgae	1543:1566	common marine macroalgae	1543:1566	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	5	79	theme	Strain	838:843	arg1	83-1					845:848	Strain 83-1	838:848	Strain 83-1	838:848	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	1	80	theme	nutrient	178:185	arg1	polysaccharides					135:149	Algal polysaccharides	129:149	Algal polysaccharides	129:149	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	80	theme	nutrient	178:185	arg1	source					187:192	an important bacterial nutrient source	155:192	an important bacterial nutrient source	155:192	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	4	81	theme	83-1	777:780	arg1	adaptations					749:759	cellular adaptations	740:759	cellular adaptations of A. macleodii 83-1	740:780	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	0	82	theme	algal	106:110	arg1	polysaccharides					112:126	algal polysaccharides	106:126	algal polysaccharides	106:126	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	8	83	theme	particular	1572:1581	arg1	Saccharina					1583:1592	particular Saccharina	1572:1592	particular Saccharina	1572:1592	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	4	84	theme	pectin	830:835	arg1	mix					799:801	a mix	797:801	a mix of laminarin, alginate and pectin	797:835	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	2	85	theme	abundant	357:364	arg1	aspects					270:276	cellular and ecological aspects	246:276	cellular and ecological aspects	246:276	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	8	86	theme	cellular	1480:1487	arg1	adaptations					1489:1499	cellular adaptations	1480:1499	cellular adaptations	1480:1499	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	11	87	theme	interactions	2102:2113	arg1	understanding					2070:2082	the understanding	2066:2082	the understanding of bacteria-algae interactions	2066:2113	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	11	87	theme	interactions	2102:2113	arg1	remineralization					2123:2138	the remineralization	2119:2138	the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling	2119:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	0	88	theme	cellular	9:16	arg1	adaptations					18:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations	0:28	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	7	89	from	changes	1237:1243	arg1	composition					1262:1272	exometabolome composition	1248:1272	exometabolome composition	1248:1272	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	2	90	dep	abundant	357:364	arg1	as					343:344	as	343:344	as	343:344	However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood.					
30116038	5	91	theme	catabolite	895:904	arg1	repression					906:915	catabolite repression	895:915	catabolite repression	895:915	Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization.					
30116038	6	92	theme	functional	1200:1209	arg1	traits					1211:1216	other functional traits	1194:1216	CAZymes/polysaccharide utilization loci but also other functional traits	1145:1216	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	11	93	theme	global	2022:2027	arg1	scales					2029:2034	global scales	2022:2034	global scales	2022:2034	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	4	94	theme	alginate	817:824	arg1	mix					799:801	a mix	797:801	a mix of laminarin, alginate and pectin	797:835	Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin.					
30116038	7	95	theme	pyrroloquinoline	1327:1342	arg1	quinone					1344:1350	pyrroloquinoline quinone	1327:1350	pyrroloquinoline quinone	1327:1350	Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community.					
30116038	1	96	theme	webs	231:234	arg1	component					206:214	central component	198:214	central component of marine food webs	198:234	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	96	theme	webs	231:234	arg1	polysaccharides					135:149	Algal polysaccharides	129:149	Algal polysaccharides	129:149	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	1	96	theme	webs	231:234	arg1	source					187:192	an important bacterial nutrient source	155:192	an important bacterial nutrient source	155:192	Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs.					
30116038	11	97	theme	algae	2013:2017	arg1	productivity					1997:2008	the substantial primary productivity	1973:2008	the substantial primary productivity of algae on global scales	1973:2034	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	8	98	theme	alginate	1625:1632	arg1	rhamnogalacturonan					1650:1667	alginate and pectin-like rhamnogalacturonan	1625:1667	alginate and pectin-like rhamnogalacturonan	1625:1667	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	3	99	theme	several	499:505	arg1	ways					507:510	several ways	499:510	several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans	499:673	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	0	100	theme	Alteromonas	61:71	arg1	macleodii					73:81	Alteromonas macleodii	61:81	Alteromonas macleodii	61:81	Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.					
30116038	10	101	theme	distinct	1881:1888	arg1	types					1916:1920	carbohydrate utilization types	1891:1920	distinct 'carbohydrate utilization types' with different ecological strategies	1881:1958	suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct 'carbohydrate utilization types' with different ecological strategies.					
30116038	3	102	theme	marine	433:438	arg1	mixtures					455:462	marine polysaccharide mixtures	433:462	marine polysaccharide mixtures	433:462	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	6	103	from	shifts	1057:1062	arg1	expression					1072:1081	gene expression	1067:1081	gene expression	1067:1081	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	6	103	from	shifts	1057:1062	arg1	abundance					1092:1100	protein abundance	1084:1100	protein abundance	1084:1100	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	6	103	from	shifts	1057:1062	arg1	secretion					1117:1125	metabolite secretion	1106:1125	metabolite secretion	1106:1125	This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits.					
30116038	8	104	theme	pectin-like	1638:1648	arg1	rhamnogalacturonan					1650:1667	alginate and pectin-like rhamnogalacturonan	1625:1667	alginate and pectin-like rhamnogalacturonan	1625:1667	The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan.					
30116038	3	105	theme	Alteromonas	538:548	arg1	83-1					560:563	the model bacterium Alteromonas macleodii 83-1	518:563	the model bacterium Alteromonas macleodii 83-1	518:563	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
30116038	11	106	theme	marine	2198:2203	arg1	cycling					2212:2218	marine carbon cycling	2198:2218	marine carbon cycling	2198:2218	Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria-algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.					
30116038	3	107	theme	model	522:526	arg1	83-1					560:563	the model bacterium Alteromonas macleodii 83-1	518:563	the model bacterium Alteromonas macleodii 83-1	518:563	Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans.					
29580412	6	0	theme	elevated	989:996	arg1	portion					998:1004	an elevated portion	986:1004	an elevated portion of 1,3-linked glucose units	986:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	1	1	theme	fruit	148:152	arg1	solution					170:177	a fruit extract/sucrose solution	146:177	a fruit extract/sucrose solution	146:177	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	5	2	theme	O2-branched	846:856	arg1	dextrans					858:865	O3- and O2-branched dextrans	838:865	O3- and O2-branched dextrans as well as lower amounts of levans	838:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	0	3	from	characterization	11:26	arg1	kefir					65:69	water kefir	59:69	water kefir	59:69	Structural characterization of the exopolysaccharides from water kefir.					
29580412	1	4	theme	extract/sucrose	154:168	arg1	solution					170:177	a fruit extract/sucrose solution	146:177	a fruit extract/sucrose solution	146:177	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	5	5	theme	beverage	799:806	arg1	exopolysaccharide-fraction					746:771	The exopolysaccharide-fraction	742:771	The exopolysaccharide-fraction of the investigated kefir beverage	742:806	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	2	6	from	exopolysaccharides	312:329	arg1	beverage					344:351	the kefir beverage	334:351	the kefir beverage	334:351	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	2	7	theme	grain	287:291	arg1	formation					293:301	the kefir grain formation	277:301	the kefir grain formation	277:301	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	3	8	theme	multiple	547:554	arg1	approaches					572:581	multiple chromatographic approaches	547:581	multiple chromatographic approaches	547:581	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	0	9	from	kefir	65:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the exopolysaccharides from water kefir.	0:70	Structural characterization of the exopolysaccharides from water kefir.					
29580412	0	9	from	kefir	65:69	arg1	exopolysaccharides					35:52	the exopolysaccharides	31:52	the exopolysaccharides from water kefir	31:69	Structural characterization of the exopolysaccharides from water kefir.					
29580412	2	10	theme	kefir	281:285	arg1	formation					293:301	the kefir grain formation	277:301	the kefir grain formation	277:301	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	1	11	theme	Water	72:76	arg1	kefir					78:82	Water kefir	72:82	Water kefir	72:82	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	1	11	theme	Water	72:76	arg1	beverage					89:96	a beverage	87:96	a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains	87:205	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	3	12	theme	kefir	457:461	arg1	grains					463:468	kefir grains	457:468	kefir grains	457:468	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	1	13	theme	solution	170:177	arg1	fermentation					130:141	fermentation	130:141	fermentation of a fruit extract/sucrose solution with insoluble kefir grains	130:205	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	1	14	with	fermentation	130:141	arg1	grains					200:205	insoluble kefir grains	184:205	insoluble kefir grains	184:205	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	4	15	theme	further	692:698	arg1	information					700:710	further information	692:710	further information about the exopolysaccharides	692:739	Furthermore, different fractionation techniques were applied to obtain further information about the exopolysaccharides.					
29580412	5	16	theme	lower	878:882	arg1	amounts					884:890	lower amounts	878:890	O3- and O2-branched dextrans as well as lower amounts of levans	838:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	5	16	theme	lower	878:882	arg1	levans					895:900	levans	895:900	levans	895:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	1	17	theme	insoluble	184:192	arg1	grains					200:205	insoluble kefir grains	184:205	insoluble kefir grains	184:205	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	0	18	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the exopolysaccharides from water kefir.	0:70	Structural characterization of the exopolysaccharides from water kefir.					
29580412	6	19	theme	soluble	1050:1056	arg1	dextrans					1058:1065	the soluble dextrans	1046:1065	the soluble dextrans	1046:1065	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	20	contain	contained	976:984	arg2	portion					998:1004	an elevated portion	986:1004	an elevated portion of 1,3-linked glucose units	986:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	20	contain	contained	976:984	arg1	O3-branched					960:970	O3-branched	960:970	O3-branched	960:970	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	20	contain	contained	976:984	arg1	dextrans					917:924	The insoluble dextrans	903:924	The insoluble dextrans from the kefir grains	903:946	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	7	21	theme	multiple	1156:1163	arg1	bacteria					1165:1172	multiple bacteria	1156:1172	multiple bacteria	1156:1172	The structurally different exopolysaccharides in water kefir suggest the involvement of multiple bacteria.					
29580412	3	22	theme	two-dimensional	587:601	arg1	spectroscopy					607:618	two-dimensional NMR spectroscopy	587:618	two-dimensional NMR spectroscopy	587:618	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	2	23	theme	structural	370:379	arg1	composition					381:391	the detailed structural composition	357:391	the detailed structural composition of the whole kefir grains	357:417	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	6	24	from	grains	941:946	arg1	O3-branched					960:970	O3-branched	960:970	O3-branched	960:970	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	24	from	grains	941:946	arg1	dextrans					917:924	The insoluble dextrans	903:924	The insoluble dextrans from the kefir grains	903:946	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	7	25	theme	bacteria	1165:1172	arg1	involvement					1141:1151	the involvement	1137:1151	the involvement of multiple bacteria	1137:1172	The structurally different exopolysaccharides in water kefir suggest the involvement of multiple bacteria.					
29580412	3	26	theme	chromatographic	556:570	arg1	approaches					572:581	multiple chromatographic approaches	547:581	multiple chromatographic approaches	547:581	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	5	27	theme	levans	895:900	arg1	amounts					884:890	lower amounts	878:890	O3- and O2-branched dextrans as well as lower amounts of levans	838:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	5	27	theme	levans	895:900	arg1	dextrans					858:865	O3- and O2-branched dextrans	838:865	O3- and O2-branched dextrans as well as lower amounts of levans	838:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	5	27	theme	levans	895:900	arg1	levans					895:900	levans	895:900	levans	895:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	2	28	theme	grains	412:417	arg1	exopolysaccharides					312:329	the exopolysaccharides	308:329	the exopolysaccharides in the kefir beverage	308:351	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	2	28	theme	grains	412:417	arg1	composition					381:391	the detailed structural composition	357:391	the detailed structural composition of the whole kefir grains	357:417	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	3	29	theme	kefir	492:496	arg1	beverage					498:505	the corresponding kefir beverage	474:505	the corresponding kefir beverage	474:505	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	7	30	from	exopolysaccharides	1095:1112	arg1	kefir					1123:1127	water kefir	1117:1127	water kefir	1117:1127	The structurally different exopolysaccharides in water kefir suggest the involvement of multiple bacteria.					
29580412	5	31	theme	investigated	780:791	arg1	beverage					799:806	the investigated kefir beverage	776:806	the investigated kefir beverage	776:806	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	6	32	theme	kefir	935:939	arg1	grains					941:946	the kefir grains	931:946	the kefir grains	931:946	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	1	33	theme	kefir	194:198	arg1	grains					200:205	insoluble kefir grains	184:205	insoluble kefir grains	184:205	Water kefir is a beverage which is produced by initiating fermentation of a fruit extract/sucrose solution with insoluble kefir grains.					
29580412	2	34	theme	kefir	406:410	arg1	grains					412:417	the whole kefir grains	396:417	the whole kefir grains	396:417	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	0	35	theme	exopolysaccharides	35:52	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the exopolysaccharides from water kefir.	0:70	Structural characterization of the exopolysaccharides from water kefir.					
29580412	6	36	theme	units	1028:1032	arg1	portion					998:1004	an elevated portion	986:1004	an elevated portion of 1,3-linked glucose units	986:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	5	37	theme	kefir	793:797	arg1	beverage					799:806	the investigated kefir beverage	776:806	the investigated kefir beverage	776:806	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	2	38	from	composition	381:391	arg1	beverage					344:351	the kefir beverage	334:351	the kefir beverage	334:351	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	2	39	theme	whole	400:404	arg1	grains					412:417	the whole kefir grains	396:417	the whole kefir grains	396:417	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	6	40	theme	glucose	1020:1026	arg1	units					1028:1032	1,3-linked glucose units	1009:1032	1,3-linked glucose units	1009:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	3	41	theme	NMR	603:605	arg1	spectroscopy					607:618	two-dimensional NMR spectroscopy	587:618	two-dimensional NMR spectroscopy	587:618	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	7	42	theme	water	1117:1121	arg1	kefir					1123:1127	water kefir	1117:1127	water kefir	1117:1127	The structurally different exopolysaccharides in water kefir suggest the involvement of multiple bacteria.					
29580412	2	43	theme	kefir	338:342	arg1	beverage					344:351	the kefir beverage	334:351	the kefir beverage	334:351	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	0	44	theme	water	59:63	arg1	kefir					65:69	water kefir	59:69	water kefir	59:69	Structural characterization of the exopolysaccharides from water kefir.					
29580412	6	45	theme	1,3-linked	1009:1018	arg1	units					1028:1032	1,3-linked glucose units	1009:1032	1,3-linked glucose units	1009:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	7	46	theme	different	1085:1093	arg1	exopolysaccharides					1095:1112	The structurally different exopolysaccharides	1068:1112	The structurally different exopolysaccharides in water kefir	1068:1127	The structurally different exopolysaccharides in water kefir suggest the involvement of multiple bacteria.					
29580412	3	47	theme	corresponding	478:490	arg1	beverage					498:505	the corresponding kefir beverage	474:505	the corresponding kefir beverage	474:505	Therefore, kefir grains and the corresponding kefir beverage were analyzed for exopolysaccharides by multiple chromatographic approaches and two-dimensional NMR spectroscopy.					
29580412	6	48	theme	insoluble	907:915	arg1	O3-branched					960:970	O3-branched	960:970	O3-branched	960:970	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	48	theme	insoluble	907:915	arg1	dextrans					917:924	The insoluble dextrans	903:924	The insoluble dextrans from the kefir grains	903:946	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	6	49	link	1,3-linked	1009:1018	arg1	units					1028:1032	1,3-linked glucose units	1009:1032	1,3-linked glucose units	1009:1032	The insoluble dextrans from the kefir grains were mostly O3-branched and contained an elevated portion of 1,3-linked glucose units compared to the soluble dextrans.					
29580412	4	50	theme	different	634:642	arg1	techniques					658:667	different fractionation techniques	634:667	different fractionation techniques	634:667	Furthermore, different fractionation techniques were applied to obtain further information about the exopolysaccharides.					
29580412	2	51	theme	major	263:267	arg1	role					269:272	a major role	261:272	a major role	261:272	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	2	52	theme	detailed	361:368	arg1	composition					381:391	the detailed structural composition	357:391	the detailed structural composition of the whole kefir grains	357:417	Exopolysaccharides that are formed from sucrose play a major role in the kefir grain formation, but the exopolysaccharides in the kefir beverage and the detailed structural composition of the whole kefir grains have not been studied yet.					
29580412	5	53	theme	O3-	838:840	arg1	dextrans					858:865	O3- and O2-branched dextrans	838:865	O3- and O2-branched dextrans as well as lower amounts of levans	838:900	The exopolysaccharide-fraction of the investigated kefir beverage was predominantly composed of O3- and O2-branched dextrans as well as lower amounts of levans.					
29580412	4	54	theme	fractionation	644:656	arg1	techniques					658:667	different fractionation techniques	634:667	different fractionation techniques	634:667	Furthermore, different fractionation techniques were applied to obtain further information about the exopolysaccharides.					
30107435	2	0	theme	isolate	569:575	arg1	G21					577:579	GC2 isolate G21	565:579	GC2 isolate G21	565:579	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	3	1	theme	novel	662:666	arg1	dehydrogenase					680:692	a novel UDP-glucose dehydrogenase	660:692	a novel UDP-glucose dehydrogenase (Ugd2)	660:699	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	3	1	theme	novel	662:666	arg1	Ugd2					695:698	Ugd2	695:698	Ugd2	695:698	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	8	2	theme	α-d-GlcpNAc-	1717:1728	arg1	linkages					1743:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	4	3	theme	NMR	978:980	arg1	spectroscopy					982:993	13C NMR spectroscopy	974:993	13C NMR spectroscopy	974:993	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	6	4	theme	first	1153:1157	arg1	sugar					1159:1163	The first sugar	1149:1163	The first sugar in the tetrasaccharide K units	1149:1194	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	4	theme	first	1153:1157	arg1	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose					1199:1242	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose	1199:1242	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others	1199:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	8	5	theme	unique	1560:1565	arg1	glycosyltransferases					1577:1596	the unique inverting glycosyltransferases	1556:1596	the unique inverting glycosyltransferases	1556:1596	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	5	6	theme	-configured	1098:1108	arg1	acetal					1127:1132	an (R)-configured 4,6-pyruvic acid acetal	1092:1132	an (R)-configured 4,6-pyruvic acid acetal added by Prt2	1092:1146	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	2	7	theme	gtr	621:623	arg1	gene					626:629	a single glycosyltransferase (gtr) gene	591:629	a single glycosyltransferase (gtr) gene	591:629	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	8	8	dep	-d-GlcpA	1704:1711	arg1	1→4					1700:1702	1→4	1700:1702	1→4	1700:1702	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	8	9	theme	-d-GlcpA	1734:1741	arg1	linkages					1743:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	1	10	from	variation	377:385	arg1	polysaccharides					402:416	the surface polysaccharides	390:416	the surface polysaccharides	390:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	11	theme	Acinetobacter	199:211	arg1	isolates					223:230	Acinetobacter baumannii isolates	199:230	Acinetobacter baumannii isolates from the major global clones, GC1 and GC2,	199:273	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	2	12	theme	glycosyltransferase	600:618	arg1	gene					626:629	a single glycosyltransferase (gtr) gene	591:629	a single glycosyltransferase (gtr) gene	591:629	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	6	13	contain	carries	1263:1269	arg1	sugar					1159:1163	The first sugar	1149:1163	The first sugar in the tetrasaccharide K units	1149:1194	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	13	contain	carries	1263:1269	arg2	group					1340:1344	a 4-N-acetyl group	1327:1344	a 4-N-acetyl group in the others	1327:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	13	contain	carries	1263:1269	arg1	d-QuipNAc4N					1245:1255	d-QuipNAc4N	1245:1255	d-QuipNAc4N	1245:1255	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	13	contain	carries	1263:1269	arg2	group					1301:1305	a 4-N-[(S)-3-hydroxybutanoyl] group	1271:1305	a 4-N-[(S)-3-hydroxybutanoyl] group in some K units	1271:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	13	contain	carries	1263:1269	arg1	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose					1199:1242	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose	1199:1242	2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others	1199:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	4	14	dep	1D	957:958	arg1	spectroscopy					982:993	13C NMR spectroscopy	974:993	13C NMR spectroscopy	974:993	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	14	dep	1D	957:958	arg1	1H					967:968	1H	967:968	1H	967:968	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	15	dep	composition	748:758	arg1	The					744:746	The	744:746	The	744:746	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	3	16	theme	putative	707:714	arg1	transferase					724:734	a putative pyruvyl transferase	705:734	a putative pyruvyl transferase (Ptr2)	705:741	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	3	16	theme	putative	707:714	arg1	Ptr2					737:740	Ptr2	737:740	Ptr2	737:740	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	0	17	theme	transferase	131:141	arg1	Ptr2					143:146	pyruvyl transferase Ptr2	123:146	pyruvyl transferase Ptr2	123:146	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	8	18	theme	retaining	1612:1620	arg1	Gtr45					1642:1646	the retaining glycosyltransferase Gtr45	1608:1646	the retaining glycosyltransferase Gtr45	1608:1646	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	2	19	from	synthesis	485:493	arg1	G21					577:579	GC2 isolate G21	565:579	GC2 isolate G21	565:579	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	2	19	from	synthesis	485:493	arg1	isolate					548:554	A. baumannii GC1 isolate A388	531:559	A. baumannii GC1 isolate A388	531:559	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	1	20	theme	surface	394:400	arg1	polysaccharides					402:416	the surface polysaccharides	390:416	the surface polysaccharides	390:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	21	theme	global	247:252	arg1	GC1					262:264	GC1	262:264	GC1	262:264	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	21	theme	global	247:252	arg1	clones					254:259	the major global clones	237:259	the major global clones	237:259	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	21	theme	global	247:252	arg1	GC2					270:272	GC2	270:272	GC2	270:272	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	4	22	theme	CPSs	842:845	arg1	repeats					817:823	the linear K20 and K21 tetrasaccharide repeats	778:823	repeats	817:823	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	22	theme	CPSs	842:845	arg1	units					828:832	K units	826:832	K units	826:832	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	22	theme	CPSs	842:845	arg1	K20					789:791	the linear K20 and K21 tetrasaccharide repeats	778:823	K20	789:791	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	6	23	theme	K	1188:1188	arg1	units					1190:1194	the tetrasaccharide K units	1168:1194	the tetrasaccharide K units	1168:1194	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	24	from	group	1301:1305	arg1	units					1317:1321	some K units	1310:1321	some K units	1310:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	24	from	group	1301:1305	arg1	others					1353:1358	the others	1349:1358	the others	1349:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	5	25	dep	-configured	1098:1108	arg1	R					1096:1096	R	1096:1096	R	1096:1096	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	6	26	theme	tetrasaccharide	1172:1186	arg1	units					1190:1194	the tetrasaccharide K units	1168:1194	the tetrasaccharide K units	1168:1194	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	4	27	theme	sugar	909:913	arg1	analyses					915:922	sugar analyses	909:922	sugar analyses	909:922	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	8	28	theme	d-GlcpNAc	1537:1545	arg1	linkages					1525:1532	the glycosidic linkages	1510:1532	the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively	1510:1764	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	8	29	theme	1→4	1730:1732	arg1	linkages					1743:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	α-d-GlcpNAc-(1→4)-d-GlcpA linkages	1717:1750	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	0	30	theme	dehydrogenase	103:115	arg1	Ugd2					117:120	UDP-glucose dehydrogenase Ugd2	91:120	UDP-glucose dehydrogenase Ugd2	91:120	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	2	31	theme	polysaccharide	507:520	arg1	synthesis					485:493	the synthesis	481:493	the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21	481:579	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	0	32	theme	Acinetobacter	0:12	arg1	baumannii					14:22	Acinetobacter baumannii K20	0:26	Acinetobacter baumannii K20	0:26	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	4	33	theme	tetrasaccharide	801:815	arg1	repeats					817:823	the linear K20 and K21 tetrasaccharide repeats	778:823	repeats	817:823	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	6	34	theme	K	1315:1315	arg1	units					1317:1321	some K units	1310:1321	some K units	1310:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	4	35	attach	isolated	847:854	arg1	G21					870:872	G21	870:872	G21	870:872	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	35	attach	isolated	847:854	arg1	A338					861:864	A338	861:864	A338	861:864	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	35	attach	isolated	847:854	arg2	CPSs					842:845	the CPSs	838:845	the CPSs isolated from A338 and G21	838:872	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	36	theme	linear	782:787	arg1	units					828:832	K units	826:832	K units	826:832	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	36	theme	linear	782:787	arg1	K20					789:791	the linear K20 and K21 tetrasaccharide repeats	778:823	K20	789:791	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	0	37	theme	K21	32:34	arg1	polysaccharide					45:58	K21 capsular polysaccharide	32:58	K21 capsular polysaccharide	32:58	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	0	38	theme	polysaccharide	45:58	arg1	structures					60:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures	0:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures	0:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	1	39	theme	phage	320:324	arg1	option					425:430	an option	422:430	an option	422:430	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	39	theme	phage	320:324	arg1	therapy					326:332	phage therapy	320:332	phage therapy	320:332	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	6	40	from	sugar	1159:1163	arg1	units					1190:1194	the tetrasaccharide K units	1168:1194	the tetrasaccharide K units	1168:1194	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	7	41	theme	K	1374:1374	arg1	polymerases					1381:1391	K unit polymerases WzyK20 and WzyK21	1374:1409	K unit polymerases WzyK20 and WzyK21	1374:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	41	theme	K	1374:1374	arg1	WzyK21					1404:1409	WzyK21	1404:1409	WzyK21	1404:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	41	theme	K	1374:1374	arg1	WzyK20					1393:1398	WzyK20	1393:1398	WzyK20	1393:1398	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	8	42	theme	K20	1456:1458	arg1	units					1468:1472	The K20 and K21 units	1452:1472	units	1468:1472	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	4	43	theme	13C	974:976	arg1	spectroscopy					982:993	13C NMR spectroscopy	974:993	13C NMR spectroscopy	974:993	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	8	44	theme	K21	1464:1466	arg1	units					1468:1472	The K20 and K21 units	1452:1472	units	1468:1472	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	6	45	theme	S	1279:1279	arg1	group					1301:1305	a 4-N-[(S)-3-hydroxybutanoyl] group	1271:1305	a 4-N-[(S)-3-hydroxybutanoyl] group in some K units	1271:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	8	46	theme	linkages	1525:1532	arg1	configuration					1493:1505	the configuration	1489:1505	the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively	1489:1764	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	4	47	theme	K	826:826	arg1	units					828:832	K units	826:832	K units	826:832	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	47	theme	K	826:826	arg1	K20					789:791	the linear K20 and K21 tetrasaccharide repeats	778:823	K20	789:791	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	3	48	theme	UDP-glucose	668:678	arg1	dehydrogenase					680:692	a novel UDP-glucose dehydrogenase	660:692	a novel UDP-glucose dehydrogenase (Ugd2)	660:699	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	3	48	theme	UDP-glucose	668:678	arg1	Ugd2					695:698	Ugd2	695:698	Ugd2	695:698	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	5	49	with	Ugd2	1066:1069	arg1	acetal					1127:1132	an (R)-configured 4,6-pyruvic acid acetal	1092:1132	an (R)-configured 4,6-pyruvic acid acetal added by Prt2	1092:1146	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	6	50	theme	4-N-acetyl	1329:1338	arg1	group					1340:1344	a 4-N-acetyl group	1327:1344	a 4-N-acetyl group in the others	1327:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	7	51	dep	polymerases	1381:1391	arg1	polymerases					1381:1391	K unit polymerases WzyK20 and WzyK21	1374:1409	K unit polymerases WzyK20 and WzyK21	1374:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	51	dep	polymerases	1381:1391	arg1	WzyK21					1404:1409	WzyK21	1404:1409	WzyK21	1404:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	51	dep	polymerases	1381:1391	arg1	WzyK20					1393:1398	WzyK20	1393:1398	WzyK20	1393:1398	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	2	52	dep	A.	531:532	arg1	baumannii					534:542	baumannii	534:542	baumannii	534:542	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	8	53	theme	linkages	1743:1750	arg1	formation					1670:1678	the formation	1666:1678	the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively	1666:1764	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	4	54	theme	K21	797:799	arg1	repeats					817:823	the linear K20 and K21 tetrasaccharide repeats	778:823	repeats	817:823	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	8	55	theme	inverting	1567:1575	arg1	glycosyltransferases					1577:1596	the unique inverting glycosyltransferases	1556:1596	the unique inverting glycosyltransferases	1556:1596	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	1	56	theme	baumannii	213:221	arg1	isolates					223:230	Acinetobacter baumannii isolates	199:230	Acinetobacter baumannii isolates from the major global clones, GC1 and GC2,	199:273	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	2	57	theme	single	593:598	arg1	gene					626:629	a single glycosyltransferase (gtr) gene	591:629	a single glycosyltransferase (gtr) gene	591:629	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	5	58	with	d-galactose	1075:1085	arg1	acetal					1127:1132	an (R)-configured 4,6-pyruvic acid acetal	1092:1132	an (R)-configured 4,6-pyruvic acid acetal added by Prt2	1092:1146	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	2	59	theme	GC1	544:546	arg1	isolate					548:554	A. baumannii GC1 isolate A388	531:559	A. baumannii GC1 isolate A388	531:559	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	3	60	theme	pyruvyl	716:722	arg1	transferase					724:734	a putative pyruvyl transferase	705:734	a putative pyruvyl transferase (Ptr2)	705:741	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	3	60	theme	pyruvyl	716:722	arg1	Ptr2					737:740	Ptr2	737:740	Ptr2	737:740	They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2).					
30107435	1	61	theme	major	241:245	arg1	GC1					262:264	GC1	262:264	GC1	262:264	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	61	theme	major	241:245	arg1	clones					254:259	the major global clones	237:259	the major global clones	237:259	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	61	theme	major	241:245	arg1	GC2					270:272	GC2	270:272	GC2	270:272	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	2	62	theme	A.	531:532	arg1	isolate					548:554	A. baumannii GC1 isolate A388	531:559	A. baumannii GC1 isolate A388	531:559	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	8	63	theme	glycosyltransferase	1622:1640	arg1	Gtr45					1642:1646	the retaining glycosyltransferase Gtr45	1608:1646	the retaining glycosyltransferase Gtr45	1608:1646	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	2	64	theme	GC2	565:567	arg1	G21					577:579	GC2 isolate G21	565:579	GC2 isolate G21	565:579	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	1	65	from	clones	254:259	arg1	isolates					223:230	Acinetobacter baumannii isolates	199:230	Acinetobacter baumannii isolates from the major global clones, GC1 and GC2,	199:273	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	6	66	theme	-3-hydroxybutanoyl	1281:1298	arg1	group					1301:1305	a 4-N-[(S)-3-hydroxybutanoyl] group	1271:1305	a 4-N-[(S)-3-hydroxybutanoyl] group in some K units	1271:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	7	67	theme	-d-Galp	1438:1444	arg1	bond					1446:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	2	68	theme	capsular	498:505	arg1	CPS					523:525	CPS	523:525	CPS	523:525	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	2	68	theme	capsular	498:505	arg1	polysaccharide					507:520	capsular polysaccharide	498:520	capsular polysaccharide (CPS)	498:526	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	5	69	theme	4,6-pyruvic	1110:1120	arg1	acetal					1127:1132	an (R)-configured 4,6-pyruvic acid acetal	1092:1132	an (R)-configured 4,6-pyruvic acid acetal added by Prt2	1092:1146	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	1	70	from	polysaccharides	402:416	arg1	knowledge					360:368	extensive knowledge	350:368	extensive knowledge of the variation in the surface polysaccharides	350:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	0	71	theme	UDP-glucose	91:101	arg1	Ugd2					117:120	UDP-glucose dehydrogenase Ugd2	91:120	UDP-glucose dehydrogenase Ugd2	91:120	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	7	72	theme	1→2	1434:1436	arg1	bond					1446:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	6	73	theme	4-N-[	1273:1277	arg1	group					1301:1305	a 4-N-[(S)-3-hydroxybutanoyl] group	1271:1305	a 4-N-[(S)-3-hydroxybutanoyl] group in some K units	1271:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	1	74	theme	extensive	350:358	arg1	knowledge					360:368	extensive knowledge	350:368	extensive knowledge of the variation in the surface polysaccharides	350:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	7	75	theme	β-d-QuipNAc4NR-	1418:1432	arg1	bond					1446:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	a β-d-QuipNAc4NR-(1→2)-d-Galp bond	1416:1449	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	0	76	theme	pyruvyl	123:129	arg1	Ptr2					143:146	pyruvyl transferase Ptr2	123:146	pyruvyl transferase Ptr2	123:146	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	8	77	theme	β-d-GlcpNAc-	1687:1698	arg1	-d-GlcpA					1704:1711	the β-d-GlcpNAc-(1→4)-d-GlcpA	1683:1711	the β-d-GlcpNAc-(1→4)-d-GlcpA	1683:1711	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	0	78	theme	baumannii	14:22	arg1	structures					60:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures	0:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures	0:69	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	1	79	theme	variation	377:385	arg1	knowledge					360:368	extensive knowledge	350:368	extensive knowledge of the variation in the surface polysaccharides	350:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	1	80	from	knowledge	360:368	arg1	polysaccharides					402:416	the surface polysaccharides	390:416	the surface polysaccharides	390:416	Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration.					
30107435	8	81	theme	-d-GlcpA	1704:1711	arg1	formation					1670:1678	the formation	1666:1678	the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively	1666:1764	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	0	82	theme	capsular	36:43	arg1	polysaccharide					45:58	K21 capsular polysaccharide	32:58	K21 capsular polysaccharide	32:58	Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases.					
30107435	4	83	theme	Smith	928:932	arg1	degradation					934:944	Smith degradation	928:944	Smith degradation	928:944	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	84	theme	repeats	817:823	arg1	structures					764:773	structures	764:773	structures	764:773	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	84	theme	repeats	817:823	arg1	composition					748:758	composition	748:758	composition	748:758	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	2	85	theme	gene	457:460	arg1	clusters					462:469	The gene clusters	453:469	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21	453:579	The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene.					
30107435	4	86	theme	K20	789:791	arg1	structures					764:773	structures	764:773	structures	764:773	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	4	86	theme	K20	789:791	arg1	composition					748:758	composition	748:758	composition	748:758	The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.					
30107435	6	87	from	group	1340:1344	arg1	units					1317:1321	some K units	1310:1321	some K units	1310:1321	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	6	87	from	group	1340:1344	arg1	others					1353:1358	the others	1349:1358	the others	1349:1358	The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-d-glucose (d-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others.					
30107435	5	88	dep	K20	1000:1002	arg1	The					996:998	The	996:998	The	996:998	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	8	89	theme	glycosidic	1514:1523	arg1	linkages					1525:1532	the glycosidic linkages	1510:1532	the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively	1510:1764	The K20 and K21 units differ only in the configuration of the glycosidic linkages of d-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-d-GlcpNAc-(1→4)-d-GlcpA and α-d-GlcpNAc-(1→4)-d-GlcpA linkages, respectively.					
30107435	5	90	theme	acid	1122:1125	arg1	acetal					1127:1132	an (R)-configured 4,6-pyruvic acid acetal	1092:1132	an (R)-configured 4,6-pyruvic acid acetal added by Prt2	1092:1146	The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and d-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2.					
30107435	7	91	theme	unit	1376:1379	arg1	polymerases					1381:1391	K unit polymerases WzyK20 and WzyK21	1374:1409	K unit polymerases WzyK20 and WzyK21	1374:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	91	theme	unit	1376:1379	arg1	WzyK21					1404:1409	WzyK21	1404:1409	WzyK21	1404:1409	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
30107435	7	91	theme	unit	1376:1379	arg1	WzyK20					1393:1398	WzyK20	1393:1398	WzyK20	1393:1398	Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-d-QuipNAc4NR-(1→2)-d-Galp bond.					
29715304	11	0	theme	simple	1512:1517	arg1	approach					1519:1526	a simple approach	1510:1526	a simple approach	1510:1526	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	6	1	theme	secondary	896:904	arg1	metabolites					906:916	other secondary metabolites	890:916	other secondary metabolites	890:916	Therefore, how to remove polysaccharides and other secondary metabolites during RNA extraction is the primary challenge.					
29715304	9	2	theme	other	1378:1382	arg1	impurities					1384:1393	other impurities	1378:1393	other impurities	1378:1393	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	3	with	clear	1290:1294	arg1	contamination					1351:1363	no obvious contamination	1340:1363	no obvious contamination with DNA and other impurities	1340:1393	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	3	with	clear	1290:1294	arg1	integrity					1326:1334	good integrity	1321:1334	good integrity	1321:1334	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	1	4	theme	investigations	244:257	arg1	basis					162:166	the basis	158:166	the basis of plant molecular biology research, plant genetics and other physiological investigations	158:257	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	6	5	theme	other	890:894	arg1	metabolites					906:916	other secondary metabolites	890:916	other secondary metabolites	890:916	Therefore, how to remove polysaccharides and other secondary metabolites during RNA extraction is the primary challenge.					
29715304	10	6	theme	gel	1425:1427	arg1	electrophoresis					1429:1443	gel electrophoresis	1425:1443	gel electrophoresis	1425:1443	And it was also evaluated by gel electrophoresis, nucleic acid quantitative detector and PCR assessment.					
29715304	4	7	from	polyphenols	643:653	arg1	rich					615:618	rich	615:618	rich	615:618	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	5	8	theme	chemical	693:700	arg1	properties					702:711	The physical and chemical properties	676:711	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites	676:797	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	4	9	dep	guaranteed	521:530	arg1	difficult					665:673	difficult	665:673	difficult	665:673	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	2	10	theme	large	274:278	arg1	number					280:285	a large number	272:285	a large number of nucleotide isolation methods	272:317	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	10	theme	large	274:278	arg1	kits					371:374	commercial kits	360:374	commercial kits	360:374	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	10	theme	large	274:278	arg1	on					402:403	on	402:403	on	402:403	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	4	11	from	polysaccharides	623:637	arg1	rich					615:618	rich	615:618	rich	615:618	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	3	12	theme	plants	437:442	arg1	nature					417:422	the nature	413:422	the nature of different plants	413:442	Due to the nature of different plants, extraction methods vary.					
29715304	0	13	theme	Dendrobium	104:113	arg1	huoshanense					115:125	Dendrobium huoshanense	104:125	Dendrobium huoshanense	104:125	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	10	14	theme	quantitative	1459:1470	arg1	detector					1472:1479	nucleic acid quantitative detector	1446:1479	nucleic acid quantitative detector	1446:1479	And it was also evaluated by gel electrophoresis, nucleic acid quantitative detector and PCR assessment.					
29715304	8	15	theme	polyphenols	1249:1259	arg1	polysaccharides					1229:1243	polysaccharides	1229:1243	polysaccharides	1229:1243	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	15	theme	polyphenols	1249:1259	arg1	amount					1219:1224	high amount	1214:1224	high amount of polysaccharides and polyphenols	1214:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	15	theme	polyphenols	1249:1259	arg1	polyphenols					1249:1259	polyphenols	1249:1259	polyphenols	1249:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	11	16	from	rich	1586:1589	arg1	polyphenols					1614:1624	polyphenols	1614:1624	polyphenols	1614:1624	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	11	16	from	rich	1586:1589	arg1	polysaccharides					1594:1608	polysaccharides	1594:1608	polysaccharides	1594:1608	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	4	17	theme	rich	615:618	arg1	plants					608:613	plants	608:613	plants rich in polysaccharides and polyphenols	608:653	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	7	18	from	metabolites	1074:1084	arg1	herb					1017:1020	an Orchidaceae perennial herb	992:1020	an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites	992:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	18	from	metabolites	1074:1084	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	18	from	metabolites	1074:1084	arg1	huoshanense					977:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	10	19	theme	acid	1454:1457	arg1	detector					1472:1479	nucleic acid quantitative detector	1446:1479	nucleic acid quantitative detector	1446:1479	And it was also evaluated by gel electrophoresis, nucleic acid quantitative detector and PCR assessment.					
29715304	7	20	from	rich	1030:1033	arg1	polysaccharides					1038:1052	polysaccharides	1038:1052	polysaccharides	1038:1052	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	20	from	rich	1030:1033	arg1	metabolites					1074:1084	other secondary metabolites	1058:1084	other secondary metabolites	1058:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	4	21	from	rich	615:618	arg1	polyphenols					643:653	polyphenols	643:653	polyphenols	643:653	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	4	21	from	rich	615:618	arg1	polysaccharides					623:637	polysaccharides	623:637	polysaccharides	623:637	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	6	22	theme	RNA	925:927	arg1	extraction					929:938	RNA extraction	925:938	RNA extraction	925:938	Therefore, how to remove polysaccharides and other secondary metabolites during RNA extraction is the primary challenge.					
29715304	5	23	theme	other	771:775	arg1	metabolites					787:797	other secondary metabolites	771:797	other secondary metabolites	771:797	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	10	24	theme	nucleic	1446:1452	arg1	detector					1472:1479	nucleic acid quantitative detector	1446:1479	nucleic acid quantitative detector	1446:1479	And it was also evaluated by gel electrophoresis, nucleic acid quantitative detector and PCR assessment.					
29715304	5	25	theme	polysaccharides	716:730	arg1	properties					702:711	The physical and chemical properties	676:711	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites	676:797	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	2	26	theme	methods	311:317	arg1	number					280:285	a large number	272:285	a large number of nucleotide isolation methods	272:317	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	26	theme	methods	311:317	arg1	kits					371:374	commercial kits	360:374	commercial kits	360:374	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	26	theme	methods	311:317	arg1	on					402:403	on	402:403	on	402:403	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	4	27	from	plants	608:613	arg1	RNA					599:601	high quality RNA	586:601	high quality RNA from plants rich in polysaccharides and polyphenols	586:653	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	1	28	theme	plant	171:175	arg1	biology					187:193	plant molecular biology research, plant genetics and other physiological investigations	171:257	biology	187:193	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	5	29	theme	secondary	777:785	arg1	metabolites					787:797	other secondary metabolites	771:797	other secondary metabolites	771:797	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	9	30	theme	good	1321:1324	arg1	integrity					1326:1334	good integrity	1321:1334	good integrity	1321:1334	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	11	31	from	suitable	1535:1542	arg1	isolation					1565:1573	RNA isolation	1561:1573	RNA isolation	1561:1573	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	2	32	theme	isolation	301:309	arg1	methods					311:317	nucleotide isolation methods	290:317	nucleotide isolation methods	290:317	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	1	33	theme	molecular	177:185	arg1	biology					187:193	plant molecular biology research, plant genetics and other physiological investigations	171:257	biology	187:193	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	4	34	theme	approach	502:509	arg1	efficiency					480:489	efficiency	480:489	efficiency of certain approach	480:509	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	7	35	theme	secondary	1064:1072	arg1	metabolites					1074:1084	other secondary metabolites	1058:1084	other secondary metabolites	1058:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	9	36	with	integrity	1326:1334	arg1	impurities					1384:1393	other impurities	1378:1393	other impurities	1378:1393	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	36	with	integrity	1326:1334	arg1	DNA					1370:1372	DNA	1370:1372	DNA	1370:1372	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	2	37	theme	nucleotide	290:299	arg1	methods					311:317	nucleotide isolation methods	290:317	nucleotide isolation methods	290:317	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	8	38	theme	polysaccharides	1229:1243	arg1	polysaccharides					1229:1243	polysaccharides	1229:1243	polysaccharides	1229:1243	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	38	theme	polysaccharides	1229:1243	arg1	amount					1219:1224	high amount	1214:1224	high amount of polysaccharides and polyphenols	1214:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	38	theme	polysaccharides	1229:1243	arg1	polyphenols					1249:1259	polyphenols	1249:1259	polyphenols	1249:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	1	39	theme	biology	187:193	arg1	basis					162:166	the basis	158:166	the basis of plant molecular biology research, plant genetics and other physiological investigations	158:257	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	7	40	from	polysaccharides	1038:1052	arg1	herb					1017:1020	an Orchidaceae perennial herb	992:1020	an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites	992:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	40	from	polysaccharides	1038:1052	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	40	from	polysaccharides	1038:1052	arg1	huoshanense					977:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	6	41	theme	primary	947:953	arg1	challenge					955:963	the primary challenge	943:963	the primary challenge	943:963	Therefore, how to remove polysaccharides and other secondary metabolites during RNA extraction is the primary challenge.					
29715304	10	42	theme	PCR	1485:1487	arg1	assessment					1489:1498	PCR assessment	1485:1498	PCR assessment	1485:1498	And it was also evaluated by gel electrophoresis, nucleic acid quantitative detector and PCR assessment.					
29715304	0	43	from	polysaccharides	66:80	arg1	rich					58:61	rich	58:61	rich	58:61	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	4	44	theme	high	586:589	arg1	RNA					599:601	high quality RNA	586:601	high quality RNA from plants rich in polysaccharides and polyphenols	586:653	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	0	45	theme	total	37:41	arg1	RNA					43:45	high-quality total RNA	24:45	high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense	24:125	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	9	46	with	contamination	1351:1363	arg1	impurities					1384:1393	other impurities	1378:1393	other impurities	1378:1393	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	46	with	contamination	1351:1363	arg1	DNA					1370:1372	DNA	1370:1372	DNA	1370:1372	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	8	47	theme	D.	1096:1097	arg1	huoshanense					1099:1109	D. huoshanense	1096:1109	D. huoshanense	1096:1109	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	47	theme	D.	1096:1097	arg1	subject					1118:1124	the subject	1114:1124	the subject	1114:1124	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	48	contain	containing	1203:1212	arg1	plants					1196:1201	plants	1196:1201	plants containing high amount of polysaccharides and polyphenols	1196:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	48	contain	containing	1203:1212	arg2	polysaccharides					1229:1243	polysaccharides	1229:1243	polysaccharides	1229:1243	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	48	contain	containing	1203:1212	arg2	polyphenols					1249:1259	polyphenols	1249:1259	polyphenols	1249:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	48	contain	containing	1203:1212	arg2	amount					1219:1224	high amount	1214:1224	high amount of polysaccharides and polyphenols	1214:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	2	49	theme	commercial	360:369	arg1	number					280:285	a large number	272:285	a large number of nucleotide isolation methods	272:317	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	49	theme	commercial	360:369	arg1	CTAB					377:380	CTAB	377:380	CTAB	377:380	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	49	theme	commercial	360:369	arg1	methods					387:393	SDS methods	383:393	SDS methods	383:393	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	49	theme	commercial	360:369	arg1	kits					371:374	commercial kits	360:374	commercial kits	360:374	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	50	theme	SDS	383:385	arg1	methods					387:393	SDS methods	383:393	SDS methods	383:393	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	2	50	theme	SDS	383:385	arg1	kits					371:374	commercial kits	360:374	commercial kits	360:374	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	0	51	theme	high-quality	24:35	arg1	RNA					43:45	high-quality total RNA	24:45	high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense	24:125	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	9	52	theme	obvious	1343:1349	arg1	contamination					1351:1363	no obvious contamination	1340:1363	no obvious contamination with DNA and other impurities	1340:1393	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	5	53	theme	physical	680:687	arg1	properties					702:711	The physical and chemical properties	676:711	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites	676:797	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	0	54	from	rich	58:61	arg1	polyphenols					86:96	polyphenols	86:96	polyphenols	86:96	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	0	54	from	rich	58:61	arg1	polysaccharides					66:80	polysaccharides	66:80	polysaccharides	66:80	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	11	55	theme	RNA	1561:1563	arg1	isolation					1565:1573	RNA isolation	1561:1573	RNA isolation	1561:1573	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	1	56	theme	plant	205:209	arg1	genetics					211:218	plant genetics	205:218	plant genetics	205:218	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	8	57	theme	high	1214:1217	arg1	polysaccharides					1229:1243	polysaccharides	1229:1243	polysaccharides	1229:1243	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	57	theme	high	1214:1217	arg1	amount					1219:1224	high amount	1214:1224	high amount of polysaccharides and polyphenols	1214:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	8	57	theme	high	1214:1217	arg1	polyphenols					1249:1259	polyphenols	1249:1259	polyphenols	1249:1259	By using D. huoshanense as the subject, we improved the method originated from CHAN and made it suitable for plants containing high amount of polysaccharides and polyphenols.					
29715304	0	58	from	plant	52:56	arg1	RNA					43:45	high-quality total RNA	24:45	high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense	24:125	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	2	59	dep	and	395:397	arg1	so					399:400	so	399:400	so	399:400	At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on.					
29715304	7	60	theme	other	1058:1062	arg1	metabolites					1074:1084	other secondary metabolites	1058:1084	other secondary metabolites	1058:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	4	61	theme	certain	494:500	arg1	approach					502:509	certain approach	494:509	certain approach	494:509	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	7	62	theme	Orchidaceae	995:1005	arg1	herb					1017:1020	an Orchidaceae perennial herb	992:1020	an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites	992:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	62	theme	Orchidaceae	995:1005	arg1	huoshanense					977:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	62	theme	Orchidaceae	995:1005	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	3	63	theme	extraction	445:454	arg1	methods					456:462	extraction methods	445:462	extraction methods	445:462	Due to the nature of different plants, extraction methods vary.					
29715304	11	64	from	polysaccharides	1594:1608	arg1	rich					1586:1589	rich	1586:1589	rich	1586:1589	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	7	65	theme	perennial	1007:1015	arg1	herb					1017:1020	an Orchidaceae perennial herb	992:1020	an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites	992:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	65	theme	perennial	1007:1015	arg1	huoshanense					977:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	65	theme	perennial	1007:1015	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	3	66	theme	different	427:435	arg1	plants					437:442	different plants	427:442	different plants	427:442	Due to the nature of different plants, extraction methods vary.					
29715304	1	67	theme	genetics	211:218	arg1	basis					162:166	the basis	158:166	the basis of plant molecular biology research, plant genetics and other physiological investigations	158:257	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	0	68	from	polyphenols	86:96	arg1	rich					58:61	rich	58:61	rich	58:61	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	11	69	theme	rich	1586:1589	arg1	plants					1579:1584	plants	1579:1584	plants rich in polysaccharides and polyphenols	1579:1624	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	1	70	theme	high	138:141	arg1	RNA					151:153	high quality RNA	138:153	high quality RNA	138:153	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	4	71	theme	different	554:562	arg1	plants					564:569	different plants	554:569	different plants	554:569	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	0	72	theme	rich	58:61	arg1	plant					52:56	plant	52:56	plant rich in polysaccharides and polyphenols using Dendrobium huoshanense	52:125	A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.					
29715304	9	73	theme	extracted	1266:1274	arg1	clear					1290:1294	clear	1290:1294	clear	1290:1294	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	73	theme	extracted	1266:1274	arg1	RNA					1282:1284	The extracted total RNA	1262:1284	The extracted total RNA	1262:1284	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	1	74	dep	biology	187:193	arg1	research					195:202	research	195:202	research	195:202	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	4	75	theme	quality	591:597	arg1	RNA					599:601	high quality RNA	586:601	high quality RNA from plants rich in polysaccharides and polyphenols	586:653	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	1	76	theme	quality	143:149	arg1	RNA					151:153	high quality RNA	138:153	high quality RNA	138:153	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	1	77	theme	other	224:228	arg1	investigations					244:257	other physiological investigations	224:257	other physiological investigations	224:257	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29715304	11	78	from	polyphenols	1614:1624	arg1	rich					1586:1589	rich	1586:1589	rich	1586:1589	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	9	79	theme	total	1276:1280	arg1	clear					1290:1294	clear	1290:1294	clear	1290:1294	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	9	79	theme	total	1276:1280	arg1	RNA					1282:1284	The extracted total RNA	1262:1284	The extracted total RNA	1262:1284	The extracted total RNA was clear and non-dispersive, with good integrity and no obvious contamination with DNA and other impurities.					
29715304	5	80	theme	nucleic	753:759	arg1	acids					761:765	nucleic acids	753:765	nucleic acids	753:765	The physical and chemical properties of polysaccharides which are similar to nucleic acids and other secondary metabolites will be coprecipitated with RNA irreversibly.					
29715304	7	81	theme	Dendrobium	966:975	arg1	herb					1017:1020	an Orchidaceae perennial herb	992:1020	an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites	992:1084	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	81	theme	Dendrobium	966:975	arg1	huoshanense					977:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense	966:987	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	7	81	theme	Dendrobium	966:975	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	Dendrobium huoshanense is an Orchidaceae perennial herb that is rich in polysaccharides and other secondary metabolites.					
29715304	4	82	theme	plants	564:569	arg1	composition					539:549	composition	539:549	composition of different plants	539:569	Moreover, efficiency of certain approach cannot be guaranteed due to composition of different plants and extracting high quality RNA from plants rich in polysaccharides and polyphenols are often difficult.					
29715304	11	83	from	isolation	1565:1573	arg1	suitable					1535:1542	suitable	1535:1542	suitable	1535:1542	Thus, as a simple approach, it is suitable and efficient in RNA isolation for plants rich in polysaccharides and polyphenols.					
29715304	1	84	theme	physiological	230:242	arg1	investigations					244:257	other physiological investigations	224:257	other physiological investigations	224:257	Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations.					
29438753	0	0	theme	STZ-induced	105:115	arg1	mice					126:129	STZ-induced diabetic mice	105:129	STZ-induced diabetic mice	105:129	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	7	1	theme	leaf	1483:1486	arg1	polysaccharides					1488:1502	leaf polysaccharides	1483:1502	leaf polysaccharides	1483:1502	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	5	2	from	glycogen	843:850	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	2	from	glycogen	843:850	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	3	3	from	distinct	542:549	arg1	terms					570:574	terms	570:574	terms of the Mws, monosaccharide compositions and functional groups	570:636	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	7	4	with	polysaccharides	1344:1358	arg1	Mws					1372:1374	higher Mws	1365:1374	higher Mws	1365:1374	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	7	4	with	polysaccharides	1344:1358	arg1	content					1388:1394	glucose content	1380:1394	glucose content	1380:1394	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	0	5	from	activities	13:22	arg1	mice					126:129	STZ-induced diabetic mice	105:129	STZ-induced diabetic mice	105:129	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	0	5	from	activities	13:22	arg1	roxburghii					62:71	Anoectochilus roxburghii	48:71	Anoectochilus roxburghii	48:71	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	0	5	from	activities	13:22	arg1	formosanus					91:100	Anoectochilus formosanus	77:100	Anoectochilus formosanus	77:100	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	5	6	from	cholesterol	868:878	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	6	from	cholesterol	868:878	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	1	7	theme	whole	189:193	arg1	plants					195:200	whole plants	189:200	whole plants	189:200	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	5	8	from	activities	1040:1049	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	8	from	activities	1040:1049	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	9	theme	mice	1074:1077	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	9	theme	mice	1074:1077	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	10	theme	low-density	905:915	arg1	LDL-C					942:946	LDL-C	942:946	LDL-C	942:946	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	10	theme	low-density	905:915	arg1	cholesterol					929:939	low-density lipoprotein cholesterol	905:939	low-density lipoprotein cholesterol (LDL-C)	905:947	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	7	11	theme	antidiabetic	1536:1547	arg1	activities					1549:1558	the best antidiabetic activities	1527:1558	the best antidiabetic activities	1527:1558	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	0	12	theme	diabetic	117:124	arg1	mice					126:129	STZ-induced diabetic mice	105:129	STZ-induced diabetic mice	105:129	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	5	13	theme	enzyme	1033:1038	arg1	activities					1040:1049	antioxidant enzyme activities	1021:1049	antioxidant enzyme activities in liver and kidney of mice	1021:1077	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	14	theme	lipoprotein	917:927	arg1	LDL-C					942:946	LDL-C	942:946	LDL-C	942:946	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	14	theme	lipoprotein	917:927	arg1	cholesterol					929:939	low-density lipoprotein cholesterol	905:939	low-density lipoprotein cholesterol (LDL-C)	905:947	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	6	15	dep	exhibited	1155:1163	arg1	related					1284:1290	related	1284:1290	related	1284:1290	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	0	16	from	roxburghii	62:71	arg1	polysaccharides					27:41	polysaccharides	27:41	polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus	27:100	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	0	16	from	roxburghii	62:71	arg1	activities					13:22	Antidiabetic activities	0:22	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.	0:130	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	6	17	theme	antihyperlipidemic	1233:1250	arg1	activities					1252:1261	antihyperlipidemic activities	1233:1261	antihyperlipidemic activities	1233:1261	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	3	18	dep	polysaccharides	501:515	arg1	polysaccharides					501:515	leaf polysaccharides	496:515	leaf polysaccharides (ARPs-2 and AFPs-2)	496:535	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	18	dep	polysaccharides	501:515	arg1	AFPs-2					529:534	AFPs-2	529:534	AFPs-2	529:534	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	18	dep	polysaccharides	501:515	arg1	ARPs-2					518:523	ARPs-2	518:523	ARPs-2	518:523	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	4	19	theme	ARPs	674:677	arg1	structures					660:669	the primary structures	648:669	the primary structures of ARPs and AFPs	648:686	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	4	19	theme	ARPs	674:677	arg1	similar					693:699	similar	693:699	similar	693:699	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	3	20	theme	Root	452:455	arg1	polysaccharides					457:471	Root polysaccharides	452:471	Root polysaccharides (ARPs-1and AFPs-1)	452:490	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	20	theme	Root	452:455	arg1	AFPs-1					484:489	ARPs-1and AFPs-1	474:489	ARPs-1and AFPs-1	474:489	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	5	21	with	feeding	773:779	arg1	samples					802:808	polysaccharides samples	786:808	polysaccharides samples	786:808	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	4	22	theme	primary	652:658	arg1	structures					660:669	the primary structures	648:669	the primary structures of ARPs and AFPs	648:686	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	4	22	theme	primary	652:658	arg1	similar					693:699	similar	693:699	similar	693:699	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	5	23	from	triglyceride	886:897	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	23	from	triglyceride	886:897	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	7	24	contain	had	1560:1562	arg1	ARPs					1509:1512	ARPs	1509:1512	ARPs which showed the best antidiabetic activities	1509:1558	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	7	24	contain	had	1560:1562	arg2	potential					1568:1576	the potential to be used as functional food or medicine for diabetes treatment	1564:1641	the potential to be used as functional food or medicine for diabetes treatment	1564:1641	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	7	25	theme	better	1447:1452	arg1	activities					1467:1476	better antidiabetic activities	1447:1476	better antidiabetic activities	1447:1476	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	2	26	theme	antidiabetic	383:394	arg1	activities					396:405	their antidiabetic activities	377:405	their antidiabetic activities	377:405	Their primary characteristics were identified and their antidiabetic activities were evaluated in STZ-induced diabetic mice.					
29438753	0	27	theme	Antidiabetic	0:11	arg1	activities					13:22	Antidiabetic activities	0:22	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.	0:130	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	7	28	theme	diabetes	1624:1631	arg1	treatment					1633:1641	diabetes treatment	1624:1641	diabetes treatment	1624:1641	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	7	29	theme	root	1339:1342	arg1	polysaccharides					1344:1358	root polysaccharides	1339:1358	root polysaccharides	1339:1358	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	0	30	theme	polysaccharides	27:41	arg1	activities					13:22	Antidiabetic activities	0:22	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.	0:130	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	5	31	theme	antidiabetic	1110:1121	arg1	activities					1123:1132	the antidiabetic activities	1106:1132	the antidiabetic activities	1106:1132	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	6	32	theme	antidiabetic	1173:1184	arg1	activities					1186:1195	varying antidiabetic activities	1165:1195	varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities)	1165:1262	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	7	33	theme	glucose	1380:1386	arg1	content					1388:1394	glucose content	1380:1394	glucose content	1380:1394	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	6	34	dep	activities	1186:1195	arg1	antioxidant					1217:1227	antioxidant	1217:1227	antioxidant	1217:1227	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	6	34	dep	activities	1186:1195	arg1	activities					1252:1261	antihyperlipidemic activities	1233:1261	antihyperlipidemic activities	1233:1261	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	6	34	dep	activities	1186:1195	arg1	antihyperglycemic					1198:1214	antihyperglycemic	1198:1214	antihyperglycemic	1198:1214	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	2	35	theme	diabetic	437:444	arg1	mice					446:449	STZ-induced diabetic mice	425:449	STZ-induced diabetic mice	425:449	Their primary characteristics were identified and their antidiabetic activities were evaluated in STZ-induced diabetic mice.					
29438753	5	36	theme	body	815:818	arg1	weight					820:825	the body weight	811:825	the body weight	811:825	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	36	theme	body	815:818	arg1	TG					900:901	TG	900:901	TG	900:901	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	1	37	dep	parts	182:186	arg1	roots					203:207	roots	203:207	roots	203:207	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	37	dep	parts	182:186	arg1	plants					195:200	whole plants	189:200	whole plants	189:200	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	37	dep	parts	182:186	arg1	leaves					213:218	leaves	213:218	leaves	213:218	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	2	38	theme	STZ-induced	425:435	arg1	mice					446:449	STZ-induced diabetic mice	425:449	STZ-induced diabetic mice	425:449	Their primary characteristics were identified and their antidiabetic activities were evaluated in STZ-induced diabetic mice.					
29438753	6	39	theme	primary	1301:1307	arg1	characteristics					1309:1323	their primary characteristics	1295:1323	their primary characteristics	1295:1323	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	7	40	theme	best	1531:1534	arg1	activities					1549:1558	the best antidiabetic activities	1527:1558	the best antidiabetic activities	1527:1558	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	5	41	theme	lipoprotein	963:973	arg1	cholesterol					975:985	high-density lipoprotein cholesterol	950:985	high-density lipoprotein cholesterol (HDL-C)	950:993	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	41	theme	lipoprotein	963:973	arg1	HDL-C					988:992	HDL-C	988:992	HDL-C	988:992	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	42	from	cholesterol	975:985	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	42	from	cholesterol	975:985	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	43	theme	blood	828:832	arg1	glucose					834:840	blood glucose	828:840	blood glucose	828:840	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	4	44	theme	AFPs	683:686	arg1	structures					660:669	the primary structures	648:669	the primary structures of ARPs and AFPs	648:686	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	4	44	theme	AFPs	683:686	arg1	similar					693:699	similar	693:699	similar	693:699	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	7	45	theme	antidiabetic	1454:1465	arg1	activities					1467:1476	better antidiabetic activities	1447:1476	better antidiabetic activities	1447:1476	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	1	46	dep	roxburghii	238:247	arg1	AFPs-1					307:312	AFPs-1	307:312	AFPs-1	307:312	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	46	dep	roxburghii	238:247	arg1	AFPs-2					318:323	AFPs-2	318:323	AFPs-2	318:323	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	46	dep	roxburghii	238:247	arg1	ARPs-2					293:298	ARPs-2	293:298	ARPs-2	293:298	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	46	dep	roxburghii	238:247	arg1	AFPs					301:304	AFPs	301:304	AFPs	301:304	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	46	dep	roxburghii	238:247	arg1	ARPs					279:282	ARPs	279:282	ARPs	279:282	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	1	46	dep	roxburghii	238:247	arg1	ARPs-1					285:290	ARPs-1	285:290	ARPs-1	285:290	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	3	47	theme	monosaccharide	588:601	arg1	compositions					603:614	monosaccharide compositions	588:614	monosaccharide compositions	588:614	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	7	48	theme	higher	1365:1370	arg1	Mws					1372:1374	higher Mws	1365:1374	higher Mws	1365:1374	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	3	49	theme	ARPs-1and	474:482	arg1	polysaccharides					457:471	Root polysaccharides	452:471	Root polysaccharides (ARPs-1and AFPs-1)	452:490	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	49	theme	ARPs-1and	474:482	arg1	AFPs-1					484:489	ARPs-1and AFPs-1	474:489	ARPs-1and AFPs-1	474:489	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	7	50	from	polysaccharides	1344:1358	arg1	roxburghii					1407:1416	A. roxburghii	1404:1416	A. roxburghii	1404:1416	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	7	50	from	polysaccharides	1344:1358	arg1	formosanus					1425:1434	A. formosanus	1422:1434	A. formosanus	1422:1434	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	5	51	theme	antioxidant	1021:1031	arg1	activities					1040:1049	antioxidant enzyme activities	1021:1049	antioxidant enzyme activities in liver and kidney of mice	1021:1077	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	52	from	glucose	834:840	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	52	from	glucose	834:840	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	2	53	theme	primary	333:339	arg1	characteristics					341:355	Their primary characteristics	327:355	Their primary characteristics	327:355	Their primary characteristics were identified and their antidiabetic activities were evaluated in STZ-induced diabetic mice.					
29438753	5	54	theme	polysaccharides	786:800	arg1	samples					802:808	polysaccharides samples	786:808	polysaccharides samples	786:808	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	3	55	theme	Mws	583:585	arg1	terms					570:574	terms	570:574	terms of the Mws, monosaccharide compositions and functional groups	570:636	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	5	56	from	weight	820:825	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	56	from	weight	820:825	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	0	57	from	formosanus	91:100	arg1	polysaccharides					27:41	polysaccharides	27:41	polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus	27:100	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	0	57	from	formosanus	91:100	arg1	activities					13:22	Antidiabetic activities	0:22	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.	0:130	Antidiabetic activities of polysaccharides from Anoectochilus roxburghii and Anoectochilus formosanus in STZ-induced diabetic mice.					
29438753	3	58	from	other	561:565	arg1	distinct					542:549	distinct	542:549	distinct	542:549	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	7	59	theme	functional	1592:1601	arg1	food					1603:1606	functional food	1592:1606	functional food	1592:1606	Furthermore, root polysaccharides with higher Mws and glucose content in both A. roxburghii and A. formosanus, exhibited better antidiabetic activities than leaf polysaccharides, and ARPs which showed the best antidiabetic activities had the potential to be used as functional food or medicine for diabetes treatment.					
29438753	6	60	theme	varying	1165:1171	arg1	activities					1186:1195	varying antidiabetic activities	1165:1195	varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities)	1165:1262	All polysaccharides exhibited varying antidiabetic activities (antihyperglycemic, antioxidant and antihyperlipidemic activities), which were closely related to their primary characteristics.					
29438753	3	61	theme	functional	620:629	arg1	groups					631:636	functional groups	620:636	functional groups	620:636	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	4	62	theme	ARPs-1and	713:721	arg1	AFPs-1					723:728	ARPs-1and AFPs-1	713:728	ARPs-1and AFPs-1	713:728	Notably, the primary structures of ARPs and AFPs were similar to those of ARPs-1and AFPs-1, respectively.					
29438753	1	63	theme	roxburghii	238:247	arg1	parts					182:186	different parts	172:186	different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2)	172:324	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	3	64	theme	groups	631:636	arg1	terms					570:574	terms	570:574	terms of the Mws, monosaccharide compositions and functional groups	570:636	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	65	theme	leaf	496:499	arg1	polysaccharides					501:515	leaf polysaccharides	496:515	leaf polysaccharides (ARPs-2 and AFPs-2)	496:535	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	65	theme	leaf	496:499	arg1	AFPs-2					529:534	AFPs-2	529:534	AFPs-2	529:534	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	65	theme	leaf	496:499	arg1	ARPs-2					518:523	ARPs-2	518:523	ARPs-2	518:523	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	3	66	from	terms	570:574	arg1	distinct					542:549	distinct	542:549	distinct	542:549	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	5	67	from	malondiadehyde	996:1009	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	67	from	malondiadehyde	996:1009	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	3	68	theme	compositions	603:614	arg1	terms					570:574	terms	570:574	terms of the Mws, monosaccharide compositions and functional groups	570:636	Root polysaccharides (ARPs-1and AFPs-1) and leaf polysaccharides (ARPs-2 and AFPs-2) were distinct from each other in terms of the Mws, monosaccharide compositions and functional groups.					
29438753	5	69	from	insulin	853:859	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	69	from	insulin	853:859	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	1	70	theme	different	172:180	arg1	parts					182:186	different parts	172:186	different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2)	172:324	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
29438753	5	71	theme	animal	748:753	arg1	experiment					755:764	animal experiment	748:764	animal experiment	748:764	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	72	theme	high-density	950:961	arg1	cholesterol					975:985	high-density lipoprotein cholesterol	950:985	high-density lipoprotein cholesterol (HDL-C)	950:993	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	72	theme	high-density	950:961	arg1	HDL-C					988:992	HDL-C	988:992	HDL-C	988:992	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	73	theme	total	862:866	arg1	cholesterol					868:878	total cholesterol	862:878	total cholesterol (TC)	862:883	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	73	theme	total	862:866	arg1	TC					881:882	TC	881:882	TC	881:882	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	74	from	cholesterol	929:939	arg1	kidney					1064:1069	kidney	1064:1069	kidney	1064:1069	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	5	74	from	cholesterol	929:939	arg1	liver					1054:1058	liver	1054:1058	liver	1054:1058	In animal experiment, after feeding with polysaccharides samples, the body weight, blood glucose, glycogen, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondiadehyde (MDA) and antioxidant enzyme activities in liver and kidney of mice were tested to investigate the antidiabetic activities.					
29438753	1	75	theme	formosanus	267:276	arg1	parts					182:186	different parts	172:186	different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2)	172:324	Six polysaccharides were extracted from different parts (whole plants, roots and leaves) of Anoectochilus roxburghii and Anoectochilus formosanus (ARPs, ARPs-1, ARPs-2, AFPs, AFPs-1 and AFPs-2).					
31569805	0	0	theme	Roots	92:96	arg1	Responses					53:61	the Dynamic Metabonomic Responses	29:61	the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride	29:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	6	1	theme	PLS-DA	1161:1166	arg1	analysis					1149:1156	the analysis	1145:1156	the analysis of PLS-DA	1145:1166	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	1	2	theme	S.	272:273	arg1	growth					299:304	S. miltiorrhiza hairy root growth	272:304	S. miltiorrhiza hairy root growth	272:304	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	0	3	theme	Hairy	86:90	arg1	Roots					92:96	Salvia miltiorrhiza Hairy Roots	66:96	Salvia miltiorrhiza Hairy Roots	66:96	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	1	4	theme	polysaccharide	384:397	arg1	PSF					409:411	PSF	409:411	PSF	409:411	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	1	4	theme	polysaccharide	384:397	arg1	fraction					399:406	the polysaccharide fraction	380:406	the polysaccharide fraction (PSF)	380:412	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	3	5	theme	chromatography-high	716:734	arg1	UHPLC-HRMSn					766:776	UHPLC-HRMSn	766:776	UHPLC-HRMSn	766:776	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	3	5	theme	chromatography-high	716:734	arg1	spectrometry					752:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry	686:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn)	686:777	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	5	6	theme	analysis	1040:1047	arg1	results					912:918	the results	908:918	the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA)	908:1053	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	0	7	theme	Polysaccharide	101:114	arg1	Fraction					116:123	Polysaccharide Fraction	101:123	Polysaccharide Fraction from Trichoderma atroviride	101:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	7	8	theme	tanshinones	1532:1542	arg1	biosynthesis					1516:1527	the biosynthesis	1512:1527	the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited	1512:1616	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	5	9	theme	hierarchical	1016:1027	arg1	HCA					1050:1052	HCA	1050:1052	HCA	1050:1052	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	9	theme	hierarchical	1016:1027	arg1	analysis					1040:1047	hierarchical clustering analysis	1016:1047	hierarchical clustering analysis (HCA)	1016:1053	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	4	10	theme	PSF-treated	834:844	arg1	samples					857:863	PSF-treated hairy root samples	834:863	PSF-treated hairy root samples	834:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	1	11	theme	hairy	288:292	arg1	growth					299:304	S. miltiorrhiza hairy root growth	272:304	S. miltiorrhiza hairy root growth	272:304	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	2	12	theme	hairy	562:566	arg1	roots					568:572	the S. miltiorrhiza hairy roots	542:572	the S. miltiorrhiza hairy roots	542:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	5	13	theme	clustering	1029:1038	arg1	HCA					1050:1052	HCA	1050:1052	HCA	1050:1052	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	13	theme	clustering	1029:1038	arg1	analysis					1040:1047	hierarchical clustering analysis	1016:1047	hierarchical clustering analysis (HCA)	1016:1053	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	3	14	theme	present	582:588	arg1	study					590:594	the present study	578:594	the present study	578:594	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	1	15	theme	root	294:297	arg1	growth					299:304	S. miltiorrhiza hairy root growth	272:304	S. miltiorrhiza hairy root growth	272:304	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	2	16	theme	miltiorrhiza	549:560	arg1	roots					568:572	the S. miltiorrhiza hairy roots	542:572	the S. miltiorrhiza hairy roots	542:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	5	17	theme	least	967:971	arg1	squares					973:979	partial least squares	959:979	partial least squares discriminant analysis (PLS-DA)	959:1010	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	4	18	theme	root	852:855	arg1	samples					857:863	PSF-treated hairy root samples	834:863	PSF-treated hairy root samples	834:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	1	19	theme	Trichoderma	187:197	arg1	fungus					225:230	an endophytic fungus	211:230	an endophytic fungus isolated from S. miltiorrhiza	211:260	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	1	19	theme	Trichoderma	187:197	arg1	atroviride					199:208	Trichoderma atroviride	187:208	Trichoderma atroviride	187:208	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	2	20	theme	S.	546:547	arg1	roots					568:572	the S. miltiorrhiza hairy roots	542:572	the S. miltiorrhiza hairy roots	542:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	2	21	theme	whole	510:514	arg1	composition					527:537	the whole metabolite composition	506:537	the whole metabolite composition of the S. miltiorrhiza hairy roots	506:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	4	22	theme	hairy	846:850	arg1	samples					857:863	PSF-treated hairy root samples	834:863	PSF-treated hairy root samples	834:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	5	23	theme	component	933:941	arg1	PCA					953:955	PCA	953:955	PCA	953:955	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	23	theme	component	933:941	arg1	analysis					943:950	principal component analysis	923:950	principal component analysis (PCA)	923:956	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	2	24	theme	metabolite	516:525	arg1	composition					527:537	the whole metabolite composition	506:537	the whole metabolite composition of the S. miltiorrhiza hairy roots	506:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	6	25	theme	differential	1296:1307	arg1	metabolites					1309:1319	33 differential metabolites	1293:1319	33 differential metabolites	1293:1319	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	7	26	from	profiling	1415:1423	arg1	response					1428:1435	response	1428:1435	response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited	1428:1616	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	5	27	theme	analysis	943:950	arg1	results					912:918	the results	908:918	the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA)	908:1053	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	0	28	theme	UHPLC-HRMSn	0:10	arg1	Analysis					12:19	UHPLC-HRMSn Analysis	0:19	UHPLC-HRMSn Analysis	0:19	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	7	29	theme	profiling	1477:1485	arg1	changes					1452:1458	the changes	1448:1458	the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited	1448:1616	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	7	29	theme	profiling	1477:1485	arg1	PSF					1440:1442	PSF	1440:1442	PSF	1440:1442	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	3	30	theme	resolution	736:745	arg1	UHPLC-HRMSn					766:776	UHPLC-HRMSn	766:776	UHPLC-HRMSn	766:776	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	3	30	theme	resolution	736:745	arg1	spectrometry					752:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry	686:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn)	686:777	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	5	31	theme	principal	923:931	arg1	PCA					953:955	PCA	953:955	PCA	953:955	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	31	theme	principal	923:931	arg1	analysis					943:950	principal component analysis	923:950	principal component analysis (PCA)	923:956	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	3	32	theme	dynamic	601:607	arg1	responses					621:629	the dynamic metabonomic responses	597:629	the dynamic metabonomic responses of S. miltiorrhiza hairy roots	597:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	6	33	theme	negative	1249:1256	arg1	models					1258:1263	the positive and negative models	1232:1263	the positive and negative models respectively	1232:1276	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	5	34	theme	analysis	994:1001	arg1	results					912:918	the results	908:918	the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA)	908:1053	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	6	35	theme	differential	1191:1202	arg1	metabolites					1204:1214	114 and 99 differential metabolites	1180:1214	114 and 99 differential metabolites	1180:1214	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	1	36	theme	endophytic	214:223	arg1	fungus					225:230	an endophytic fungus	211:230	an endophytic fungus isolated from S. miltiorrhiza	211:260	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	1	36	theme	endophytic	214:223	arg1	atroviride					199:208	Trichoderma atroviride	187:208	Trichoderma atroviride	187:208	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	6	37	theme	metabolites	1204:1214	arg1	total					1171:1175	a total	1169:1175	a total of 114 and 99 differential metabolites	1169:1214	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	6	38	theme	positive	1236:1243	arg1	models					1258:1263	the positive and negative models	1232:1263	the positive and negative models respectively	1232:1276	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	0	39	theme	Trichoderma	130:140	arg1	atroviride					142:151	Trichoderma atroviride	130:151	Trichoderma atroviride	130:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	3	40	theme	ultra-high-performance	686:707	arg1	UHPLC-HRMSn					766:776	UHPLC-HRMSn	766:776	UHPLC-HRMSn	766:776	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	3	40	theme	ultra-high-performance	686:707	arg1	spectrometry					752:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry	686:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn)	686:777	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	5	41	theme	partial	959:965	arg1	squares					973:979	partial least squares	959:979	partial least squares discriminant analysis (PLS-DA)	959:1010	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	0	42	theme	Metabonomic	41:51	arg1	Responses					53:61	the Dynamic Metabonomic Responses	29:61	the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride	29:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	5	43	theme	squares	973:979	arg1	PLS-DA					1004:1009	PLS-DA	1004:1009	PLS-DA	1004:1009	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	43	theme	squares	973:979	arg1	analysis					994:1001	partial least squares discriminant analysis	959:1001	partial least squares discriminant analysis (PLS-DA)	959:1010	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	7	44	dep	S.	1344:1345	arg1	miltiorrhiza					1347:1358	miltiorrhiza	1347:1358	miltiorrhiza	1347:1358	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	3	45	theme	liquid	709:714	arg1	UHPLC-HRMSn					766:776	UHPLC-HRMSn	766:776	UHPLC-HRMSn	766:776	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	3	45	theme	liquid	709:714	arg1	spectrometry					752:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry	686:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn)	686:777	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	5	46	theme	PSF-treated	1070:1080	arg1	samples					1082:1088	PSF-treated samples	1070:1088	PSF-treated samples	1070:1088	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	7	47	theme	phenolic	1579:1586	arg1	acids					1588:1592	phenolic acids	1579:1592	phenolic acids	1579:1592	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	7	48	theme	S.	1344:1345	arg1	roots					1366:1370	S. miltiorrhiza hairy roots	1344:1370	S. miltiorrhiza hairy roots	1344:1370	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	0	49	theme	Dynamic	33:39	arg1	Responses					53:61	the Dynamic Metabonomic Responses	29:61	the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride	29:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	7	50	theme	acids	1588:1592	arg1	biosynthesis					1563:1574	the biosynthesis	1559:1574	the biosynthesis of phenolic acids	1559:1592	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	3	51	theme	S.	634:635	arg1	roots					656:660	S. miltiorrhiza hairy roots	634:660	S. miltiorrhiza hairy roots	634:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	2	52	theme	roots	568:572	arg1	composition					527:537	the whole metabolite composition	506:537	the whole metabolite composition of the S. miltiorrhiza hairy roots	506:572	However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots.					
31569805	5	53	from	control	1128:1134	arg1	different					1109:1117	different	1109:1117	different	1109:1117	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	3	54	theme	metabonomic	609:619	arg1	responses					621:629	the dynamic metabonomic responses	597:629	the dynamic metabonomic responses of S. miltiorrhiza hairy roots	597:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	4	55	theme	typical	791:797	arg1	TICs					825:828	TICs	825:828	TICs	825:828	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	55	theme	typical	791:797	arg1	chromatograms					810:822	UHPLC-HRMS typical total ions chromatograms	780:822	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples	780:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	3	56	theme	mass	747:750	arg1	UHPLC-HRMSn					766:776	UHPLC-HRMSn	766:776	UHPLC-HRMSn	766:776	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	3	56	theme	mass	747:750	arg1	spectrometry					752:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry	686:763	ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn)	686:777	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	6	57	theme	metabolites	1309:1319	arg1	total					1284:1288	a total	1282:1288	a total of 33 differential metabolites	1282:1319	Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified.					
31569805	4	58	from	control	889:895	arg1	different					870:878	different	870:878	different	870:878	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	59	theme	UHPLC-HRMS	780:789	arg1	TICs					825:828	TICs	825:828	TICs	825:828	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	59	theme	UHPLC-HRMS	780:789	arg1	chromatograms					810:822	UHPLC-HRMS typical total ions chromatograms	780:822	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples	780:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	1	60	theme	tanshinones	355:365	arg1	biosynthesis					339:350	the biosynthesis	335:350	the biosynthesis of tanshinones	335:365	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	7	61	theme	metabolic	1405:1413	arg1	profiling					1415:1423	the metabolic profiling	1401:1423	the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited	1401:1616	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	3	62	theme	roots	656:660	arg1	responses					621:629	the dynamic metabonomic responses	597:629	the dynamic metabonomic responses of S. miltiorrhiza hairy roots	597:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	4	63	theme	ions	805:808	arg1	TICs					825:828	TICs	825:828	TICs	825:828	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	63	theme	ions	805:808	arg1	chromatograms					810:822	UHPLC-HRMS typical total ions chromatograms	780:822	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples	780:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	7	64	theme	metabolic	1467:1475	arg1	profiling					1477:1485	the metabolic profiling	1463:1485	the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited	1463:1616	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	1	65	dep	S.	272:273	arg1	miltiorrhiza					275:286	miltiorrhiza	275:286	miltiorrhiza	275:286	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	4	66	theme	samples	857:863	arg1	TICs					825:828	TICs	825:828	TICs	825:828	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	66	theme	samples	857:863	arg1	chromatograms					810:822	UHPLC-HRMS typical total ions chromatograms	780:822	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples	780:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	67	theme	total	799:803	arg1	TICs					825:828	TICs	825:828	TICs	825:828	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	4	67	theme	total	799:803	arg1	chromatograms					810:822	UHPLC-HRMS typical total ions chromatograms	780:822	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples	780:863	UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control.					
31569805	5	68	theme	discriminant	981:992	arg1	PLS-DA					1004:1009	PLS-DA	1004:1009	PLS-DA	1004:1009	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	5	68	theme	discriminant	981:992	arg1	analysis					994:1001	partial least squares discriminant analysis	959:1001	partial least squares discriminant analysis (PLS-DA)	959:1010	Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control.					
31569805	0	69	theme	miltiorrhiza	73:84	arg1	Roots					92:96	Salvia miltiorrhiza Hairy Roots	66:96	Salvia miltiorrhiza Hairy Roots	66:96	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	1	70	attach	isolated	232:239	arg2	fungus					225:230	an endophytic fungus	211:230	an endophytic fungus isolated from S. miltiorrhiza	211:260	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	1	70	attach	isolated	232:239	arg1	miltiorrhiza					249:260	S. miltiorrhiza	246:260	S. miltiorrhiza	246:260	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	1	70	attach	isolated	232:239	arg2	atroviride					199:208	Trichoderma atroviride	187:208	Trichoderma atroviride	187:208	We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF).					
31569805	3	71	theme	miltiorrhiza	637:648	arg1	roots					656:660	S. miltiorrhiza hairy roots	634:660	S. miltiorrhiza hairy roots	634:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
31569805	7	72	theme	hairy	1360:1364	arg1	roots					1366:1370	S. miltiorrhiza hairy roots	1344:1370	S. miltiorrhiza hairy roots	1344:1370	Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.					
31569805	0	73	from	atroviride	142:151	arg1	Fraction					116:123	Polysaccharide Fraction	101:123	Polysaccharide Fraction from Trichoderma atroviride	101:151	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	0	74	theme	Salvia	66:71	arg1	Roots					92:96	Salvia miltiorrhiza Hairy Roots	66:96	Salvia miltiorrhiza Hairy Roots	66:96	UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride.					
31569805	3	75	theme	hairy	650:654	arg1	roots					656:660	S. miltiorrhiza hairy roots	634:660	S. miltiorrhiza hairy roots	634:660	In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn).					
29660895	6	0	theme	mean	1088:1091	arg1	values					1103:1108	The highest mean viscosity values	1076:1108	The highest mean viscosity values	1076:1108	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	3	1	theme	aqua	486:489	arg1	composition					471:481	the precise composition	459:481	the precise composition of aqua feeds	459:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	6	2	theme	gum	1171:1173	arg1	ratio					1157:1161	a 2:1 ratio	1151:1161	a 2:1 ratio of tara gum	1151:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	1	3	theme	water	250:254	arg1	content					256:262	extremely low water content	236:262	extremely low water content	236:262	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	4	4	theme	Dietary	650:656	arg1	supplements					658:668	Dietary supplements	650:668	Dietary supplements known as binders that enhance the stability of faeces	650:722	Dietary supplements known as binders that enhance the stability of faeces have the potential to mitigate these issues, but may also bring negative effects.					
29660895	6	5	theme	viscous	1223:1229	arg1	times					1212:1216	chyme four times	1201:1216	chyme four times more viscous than a control formulation containing the same amount of water	1201:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	3	6	theme	unpredictable	507:519	arg1	consequences					521:532	unpredictable consequences	507:532	unpredictable consequences for water quality	507:550	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	6	7	theme	2:1	1153:1155	arg1	ratio					1157:1161	a 2:1 ratio	1151:1161	a 2:1 ratio of tara gum	1151:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	5	8	theme	in	857:858	arg1	model					866:870	an in vitro model	854:870	an in vitro model to perform the first fundamental rheological characterization of salmonid chyme	854:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	7	9	theme	frequency	1361:1369	arg1	measurements					1377:1388	the frequency sweep measurements	1357:1388	the frequency sweep measurements	1357:1388	Shear resistance was quantified by analyses of slopes fitting the frequency sweep measurements.					
29660895	4	10	contain	have	724:727	arg1	supplements					658:668	Dietary supplements	650:668	Dietary supplements known as binders that enhance the stability of faeces	650:722	Dietary supplements known as binders that enhance the stability of faeces have the potential to mitigate these issues, but may also bring negative effects.					
29660895	4	10	contain	have	724:727	arg2	potential					733:741	the potential to mitigate these issues	729:766	the potential to mitigate these issues	729:766	Dietary supplements known as binders that enhance the stability of faeces have the potential to mitigate these issues, but may also bring negative effects.					
29660895	5	11	theme	substances	1056:1065	arg1	presence					1023:1030	the presence	1019:1030	the presence of rheologically active substances	1019:1065	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	1	12	theme	BACKGROUND	110:119	arg1	Developments					121:132	BACKGROUND Developments	110:132	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years	110:202	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	6	13	theme	water	1288:1292	arg1	amount					1278:1283	the same amount	1269:1283	the same amount of water	1269:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	13	theme	water	1288:1292	arg1	water					1288:1292	water	1288:1292	water	1288:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	5	14	theme	present	832:838	arg1	study					840:844	The present study	828:844	The present study	828:844	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	8	15	theme	statistical	1425:1435	arg1	approach					1437:1444	a new statistical approach	1419:1444	a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish	1419:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	5	16	theme	presence	1023:1030	arg1	impacts					1008:1014	the impacts	1004:1014	the impacts of the presence of rheologically active substances	1004:1065	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	3	17	theme	plant	564:568	arg1	ingredients					570:580	some plant ingredients	559:580	some plant ingredients	559:580	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	5	18	theme	first	887:891	arg1	characterization					917:932	the first fundamental rheological characterization	883:932	the first fundamental rheological characterization of salmonid chyme	883:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	4	19	theme	faeces	717:722	arg1	stability					704:712	the stability	700:712	the stability of faeces	700:722	Dietary supplements known as binders that enhance the stability of faeces have the potential to mitigate these issues, but may also bring negative effects.					
29660895	5	20	theme	rheological	905:915	arg1	characterization					917:932	the first fundamental rheological characterization	883:932	the first fundamental rheological characterization of salmonid chyme	883:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	0	21	from	changes	81:87	arg1	composition					97:107	feed composition	92:107	feed composition	92:107	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	6	22	theme	xanthan	1175:1181	arg1	gum					1183:1185	xanthan gum	1175:1185	xanthan gum	1175:1185	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	0	23	theme	salmonid	54:61	arg1	chyme					63:67	salmonid chyme	54:67	salmonid chyme	54:67	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	1	24	theme	based	300:304	arg1	ingredients					306:316	mainly marine fish based ingredients	281:316	mainly marine fish based ingredients	281:316	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	1	25	theme	aquacultural	155:166	arg1	salmonid					168:175	aquacultural salmonid feeds	155:181	aquacultural salmonid feeds	155:181	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	8	26	theme	changes	1494:1500	arg1	impacts					1537:1543	their impacts	1531:1543	their impacts on the nutrition, health or performance of farmed fish	1531:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	26	theme	changes	1494:1500	arg1	consequences					1464:1475	the consequences	1460:1475	the consequences of market-driven changes in aqua feed composition	1460:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	27	dep	CONCLUSIONS	1391:1401	arg1	open					1414:1417	open	1414:1417	open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish	1414:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	28	theme	aqua	1505:1508	arg1	composition					1515:1525	aqua feed composition	1505:1525	aqua feed composition	1505:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	3	29	from	reductions	600:609	arg1	stability					629:637	the mechanical stability	614:637	the mechanical stability of faeces	614:647	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	0	30	theme	feed	92:95	arg1	composition					97:107	feed composition	92:107	feed composition	92:107	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	1	31	from	Developments	121:132	arg1	production					141:150	the production	137:150	the production of aquacultural salmonid feeds in the last 20 years	137:202	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	5	32	theme	factorial	959:967	arg1	experiment					969:978	a factorial experiment	957:978	a factorial experiment designed to investigate the impacts of the presence of rheologically active substances	957:1065	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	2	33	theme	protein	419:425	arg1	market					427:432	the highly dynamic plant protein market	394:432	the highly dynamic plant protein market	394:432	These changes expose the industry to the vagaries of the highly dynamic plant protein market.					
29660895	0	34	theme	Rheological	0:10	arg1	characterization					12:27	Rheological characterization	0:27	Rheological characterization of an in vitro model for salmonid chyme	0:67	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	3	35	dep	aqua	486:489	arg1	feeds					491:495	feeds	491:495	feeds	491:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	2	36	theme	dynamic	405:411	arg1	market					427:432	the highly dynamic plant protein market	394:432	the highly dynamic plant protein market	394:432	These changes expose the industry to the vagaries of the highly dynamic plant protein market.					
29660895	3	37	theme	Resulting	435:443	arg1	variations					445:454	Resulting variations	435:454	Resulting variations in the precise composition of aqua feeds	435:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	5	38	theme	chyme	946:950	arg1	characterization					917:932	the first fundamental rheological characterization	883:932	the first fundamental rheological characterization of salmonid chyme	883:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	3	39	theme	faeces	642:647	arg1	stability					629:637	the mechanical stability	614:637	the mechanical stability of faeces	614:647	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	8	40	from	composition	1515:1525	arg1	impacts					1537:1543	their impacts	1531:1543	their impacts on the nutrition, health or performance of farmed fish	1531:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	40	from	composition	1515:1525	arg1	consequences					1464:1475	the consequences	1460:1475	the consequences of market-driven changes in aqua feed composition	1460:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	0	41	theme	in	35:36	arg1	model					44:48	an in vitro model	32:48	an in vitro model for salmonid chyme	32:67	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	3	42	contain	carry	501:505	arg2	consequences					521:532	unpredictable consequences	507:532	unpredictable consequences for water quality	507:550	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	3	42	contain	carry	501:505	arg1	variations					445:454	Resulting variations	435:454	Resulting variations in the precise composition of aqua feeds	435:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	1	43	dep	salmonid	168:175	arg1	feeds					177:181	feeds	177:181	feeds	177:181	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	8	44	from	changes	1494:1500	arg1	composition					1515:1525	aqua feed composition	1505:1525	aqua feed composition	1505:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	3	45	theme	precise	463:469	arg1	composition					471:481	the precise composition	459:481	the precise composition of aqua feeds	459:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	0	46	theme	model	44:48	arg1	characterization					12:27	Rheological characterization	0:27	Rheological characterization of an in vitro model for salmonid chyme	0:67	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	1	47	theme	marine	288:293	arg1	ingredients					306:316	mainly marine fish based ingredients	281:316	mainly marine fish based ingredients	281:316	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	0	48	dep	in	35:36	arg1	vitro					38:42	vitro	38:42	vitro	38:42	Rheological characterization of an in vitro model for salmonid chyme to quantify changes in feed composition.					
29660895	4	49	theme	negative	788:795	arg1	effects					797:803	negative effects	788:803	negative effects	788:803	Dietary supplements known as binders that enhance the stability of faeces have the potential to mitigate these issues, but may also bring negative effects.					
29660895	1	50	theme	extruded	216:223	arg1	diets					225:229	extruded diets	216:229	extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content	216:338	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	6	51	theme	viscosity	1093:1101	arg1	values					1103:1108	The highest mean viscosity values	1076:1108	The highest mean viscosity values	1076:1108	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	8	52	from	impacts	1537:1543	arg1	health					1563:1568	health	1563:1568	health	1563:1568	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	52	from	impacts	1537:1543	arg1	performance					1573:1583	performance	1573:1583	performance	1573:1583	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	52	from	impacts	1537:1543	arg1	composition					1515:1525	aqua feed composition	1505:1525	aqua feed composition	1505:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	52	from	impacts	1537:1543	arg1	nutrition					1552:1560	nutrition	1552:1560	nutrition	1552:1560	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	53	theme	farmed	1588:1593	arg1	fish					1595:1598	farmed fish	1588:1598	farmed fish	1588:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	5	54	dep	OBJECTIVE	806:814	arg1	employs					846:852	employs	846:852	employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme	846:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	6	55	theme	highest	1080:1086	arg1	values					1103:1108	The highest mean viscosity values	1076:1108	The highest mean viscosity values	1076:1108	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	56	contain	containing	1140:1149	arg1	treatment					1130:1138	a treatment	1128:1138	a treatment containing a 2:1 ratio of tara gum	1128:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	56	contain	containing	1140:1149	arg2	ratio					1157:1161	a 2:1 ratio	1151:1161	a 2:1 ratio of tara gum	1151:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	57	theme	control	1238:1244	arg1	formulation					1246:1256	a control formulation	1236:1256	a control formulation containing the same amount of water	1236:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	1	58	theme	low	246:248	arg1	content					256:262	extremely low water content	236:262	extremely low water content	236:262	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	6	59	theme	tara	1166:1169	arg1	gum					1171:1173	tara gum	1166:1173	tara gum	1166:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	1	60	theme	salmonid	168:175	arg1	production					141:150	the production	137:150	the production of aquacultural salmonid feeds in the last 20 years	137:202	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	1	61	from	ingredients	306:316	arg1	content					256:262	extremely low water content	236:262	extremely low water content	236:262	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	1	61	from	ingredients	306:316	arg1	shift					270:274	a shift	268:274	a shift from mainly marine fish based ingredients towards plant content	268:338	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	3	62	theme	water	538:542	arg1	quality					544:550	water quality	538:550	water quality	538:550	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	1	63	theme	last	190:193	arg1	years					198:202	the last 20 years	186:202	the last 20 years	186:202	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	8	64	theme	new	1421:1423	arg1	approach					1437:1444	a new statistical approach	1419:1444	a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish	1419:1598	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	7	65	theme	sweep	1371:1375	arg1	measurements					1377:1388	the frequency sweep measurements	1357:1388	the frequency sweep measurements	1357:1388	Shear resistance was quantified by analyses of slopes fitting the frequency sweep measurements.					
29660895	6	66	theme	chyme	1201:1205	arg1	times					1212:1216	chyme four times	1201:1216	chyme four times more viscous than a control formulation containing the same amount of water	1201:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	8	67	dep	nutrition	1552:1560	arg1	the					1548:1550	the	1548:1550	the	1548:1550	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	7	68	theme	slopes	1342:1347	arg1	analyses					1330:1337	analyses	1330:1337	analyses of slopes fitting the frequency sweep measurements	1330:1388	Shear resistance was quantified by analyses of slopes fitting the frequency sweep measurements.					
29660895	6	69	theme	same	1273:1276	arg1	amount					1278:1283	the same amount	1269:1283	the same amount of water	1269:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	69	theme	same	1273:1276	arg1	water					1288:1292	water	1288:1292	water	1288:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	5	70	theme	fundamental	893:903	arg1	characterization					917:932	the first fundamental rheological characterization	883:932	the first fundamental rheological characterization of salmonid chyme	883:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	3	71	from	variations	445:454	arg1	composition					471:481	the precise composition	459:481	the precise composition of aqua feeds	459:495	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	5	72	theme	active	1049:1054	arg1	substances					1056:1065	rheologically active substances	1035:1065	rheologically active substances	1035:1065	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	3	73	theme	undesirable	588:598	arg1	reductions					600:609	undesirable reductions	588:609	undesirable reductions in the mechanical stability of faeces	588:647	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	8	74	theme	market-driven	1480:1492	arg1	changes					1494:1500	market-driven changes	1480:1500	market-driven changes in aqua feed composition	1480:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	75	from	consequences	1464:1475	arg1	health					1563:1568	health	1563:1568	health	1563:1568	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	75	from	consequences	1464:1475	arg1	performance					1573:1583	performance	1573:1583	performance	1573:1583	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	75	from	consequences	1464:1475	arg1	composition					1515:1525	aqua feed composition	1505:1525	aqua feed composition	1505:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	75	from	consequences	1464:1475	arg1	nutrition					1552:1560	nutrition	1552:1560	nutrition	1552:1560	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	1	76	theme	plant	326:330	arg1	content					332:338	plant content	326:338	plant content	326:338	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	8	77	theme	feed	1510:1513	arg1	composition					1515:1525	aqua feed composition	1505:1525	aqua feed composition	1505:1525	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	1	78	from	production	141:150	arg1	years					198:202	the last 20 years	186:202	the last 20 years	186:202	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	7	79	theme	Shear	1295:1299	arg1	resistance					1301:1310	Shear resistance	1295:1310	Shear resistance	1295:1310	Shear resistance was quantified by analyses of slopes fitting the frequency sweep measurements.					
29660895	6	80	contain	containing	1258:1267	arg1	formulation					1246:1256	a control formulation	1236:1256	a control formulation containing the same amount of water	1236:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	80	contain	containing	1258:1267	arg2	water					1288:1292	water	1288:1292	water	1288:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	80	contain	containing	1258:1267	arg2	amount					1278:1283	the same amount	1269:1283	the same amount of water	1269:1292	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	2	81	theme	plant	413:417	arg1	market					427:432	the highly dynamic plant protein market	394:432	the highly dynamic plant protein market	394:432	These changes expose the industry to the vagaries of the highly dynamic plant protein market.					
29660895	5	82	theme	salmonid	937:944	arg1	chyme					946:950	salmonid chyme	937:950	salmonid chyme	937:950	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	6	83	dep	RESULTS	1068:1074	arg1	gum					1183:1185	xanthan gum	1175:1185	xanthan gum	1175:1185	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	6	83	dep	RESULTS	1068:1074	arg1	measured					1115:1122	measured	1115:1122	were measured for a treatment containing a 2:1 ratio of tara gum	1110:1173	RESULTS The highest mean viscosity values were measured for a treatment containing a 2:1 ratio of tara gum:xanthan gum, resulting in chyme four times more viscous than a control formulation containing the same amount of water.					
29660895	3	84	theme	mechanical	618:627	arg1	stability					629:637	the mechanical stability	614:637	the mechanical stability of faeces	614:647	Resulting variations in the precise composition of aqua feeds may carry unpredictable consequences for water quality, since some plant ingredients cause undesirable reductions in the mechanical stability of faeces.					
29660895	5	85	dep	in	857:858	arg1	vitro					860:864	vitro	860:864	vitro	860:864	OBJECTIVE AND METHODS The present study employs an in vitro model to perform the first fundamental rheological characterization of salmonid chyme, and a factorial experiment designed to investigate the impacts of the presence of rheologically active substances.					
29660895	2	86	theme	market	427:432	arg1	vagaries					382:389	the vagaries	378:389	the vagaries of the highly dynamic plant protein market	378:432	These changes expose the industry to the vagaries of the highly dynamic plant protein market.					
29660895	1	87	theme	fish	295:298	arg1	ingredients					306:316	mainly marine fish based ingredients	281:316	mainly marine fish based ingredients	281:316	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	1	88	with	diets	225:229	arg1	content					256:262	extremely low water content	236:262	extremely low water content	236:262	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	1	88	with	diets	225:229	arg1	shift					270:274	a shift	268:274	a shift from mainly marine fish based ingredients towards plant content	268:338	BACKGROUND Developments in the production of aquacultural salmonid feeds in the last 20 years have led to extruded diets with extremely low water content and a shift from mainly marine fish based ingredients towards plant content.					
29660895	8	89	theme	fish	1595:1598	arg1	performance					1573:1583	performance	1573:1583	performance	1573:1583	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	89	theme	fish	1595:1598	arg1	health					1563:1568	health	1563:1568	health	1563:1568	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29660895	8	89	theme	fish	1595:1598	arg1	nutrition					1552:1560	nutrition	1552:1560	nutrition	1552:1560	CONCLUSIONS These data open a new statistical approach to monitoring the consequences of market-driven changes in aqua feed composition and their impacts on the nutrition, health or performance of farmed fish.					
29939404	11	0	theme	molecular	1764:1772	arg1	sites					1803:1807	unexposed active sites	1786:1807	unexposed active sites	1786:1807	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	0	theme	molecular	1764:1772	arg1	factors					1829:1835	the main factors	1820:1835	the main factors that limit their use in functional foods	1820:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	0	theme	molecular	1764:1772	arg1	weights					1774:1780	high molecular weights	1759:1780	high molecular weights	1759:1780	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	9	1	theme	1,1-diphenyl-2-picrylhydrazyl	1514:1542	arg1	radicals					1544:1551	scavenging 1,1-diphenyl-2-picrylhydrazyl radicals	1503:1551	scavenging 1,1-diphenyl-2-picrylhydrazyl radicals	1503:1551	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	2	2	from	frondosa	294:301	arg1	enzymes					273:279	intracellular enzymes	259:279	intracellular enzymes from Grifola frondosa	259:301	In this study, we utilized intracellular enzymes from Grifola frondosa to modify RBPSs, which were extracted from RB using ultrasound.					
29939404	2	3	used	utilized	250:257	arg2	we					247:248	we	247:248	we	247:248	In this study, we utilized intracellular enzymes from Grifola frondosa to modify RBPSs, which were extracted from RB using ultrasound.					
29939404	11	4	theme	high	1759:1762	arg1	sites					1803:1807	unexposed active sites	1786:1807	unexposed active sites	1786:1807	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	4	theme	high	1759:1762	arg1	factors					1829:1835	the main factors	1820:1835	the main factors that limit their use in functional foods	1820:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	4	theme	high	1759:1762	arg1	weights					1774:1780	high molecular weights	1759:1780	high molecular weights	1759:1780	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	4	5	theme	mRBPSs-enhanced	740:754	arg1	cytotoxicity					764:775	mRBPSs-enhanced NK-cell cytotoxicity	740:775	mRBPSs-enhanced NK-cell cytotoxicity	740:775	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	9	6	theme	free	1566:1569	arg1	radicals					1571:1578	hydroxyl free radicals	1557:1578	hydroxyl free radicals	1557:1578	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	12	7	theme	monosaccharide	2027:2040	arg1	composition					2042:2052	monosaccharide composition	2027:2052	monosaccharide composition of RBPSs	2027:2061	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	3	8	theme	L9	509:510	arg1	test					503:506	an orthogonal test	489:506	an orthogonal test (L9 [3]4 )	489:517	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	3	8	theme	L9	509:510	arg1	[3					512:513	L9 [3]4	509:515	L9 [3]4	509:515	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	5	9	theme	time	930:933	arg1	ratio					848:852	polysaccharide ratio	833:852	polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	833:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	2	10	theme	intracellular	259:271	arg1	enzymes					273:279	intracellular enzymes	259:279	intracellular enzymes from Grifola frondosa	259:301	In this study, we utilized intracellular enzymes from Grifola frondosa to modify RBPSs, which were extracted from RB using ultrasound.					
29939404	1	11	theme	active	193:198	arg1	polysaccharides					155:169	Rice bran polysaccharides	145:169	Rice bran polysaccharides (RBPSs)	145:177	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	11	theme	active	193:198	arg1	constituents					200:211	the major active constituents	183:211	the major active constituents of rice bran (RB)	183:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	8	12	theme	liquid	1288:1293	arg1	chromatography					1295:1308	High-performance liquid chromatography	1271:1308	High-performance liquid chromatography results	1271:1316	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	11	13	theme	active	1796:1801	arg1	sites					1803:1807	unexposed active sites	1786:1807	unexposed active sites	1786:1807	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	13	theme	active	1796:1801	arg1	factors					1829:1835	the main factors	1820:1835	the main factors that limit their use in functional foods	1820:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	13	theme	active	1796:1801	arg1	weights					1774:1780	high molecular weights	1759:1780	high molecular weights	1759:1780	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	7	14	theme	molar	1194:1198	arg1	ratio					1200:1204	a molar ratio	1192:1204	a molar ratio	1192:1204	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	7	14	theme	molar	1194:1198	arg1	8:13:8:5:44:44					1235:1248	8:13:8:5:44:44	1235:1248	8:13:8:5:44:44	1235:1248	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	8	15	dep	105	1411:1413	arg1	to					1408:1409	to	1408:1409	to	1408:1409	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	10	16	theme	biological	1678:1687	arg1	activities					1689:1698	many biological activities	1673:1698	many biological activities	1673:1698	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	8	17	theme	molecular	1328:1336	arg1	weights					1338:1344	molecular weights	1328:1344	molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification	1328:1435	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	6	18	theme	%	1054:1054	arg1	%					1062:1062	12.01% ± 0.08%	1049:1062	12.01% ± 0.08%	1049:1062	By optimizing the conditions, the NK-cell cytotoxicity induced by mRBPSs6 was the highest, increasing by 12.01% ± 0.08%.					
29939404	5	19	theme	polysaccharide	833:846	arg1	ratio					848:852	polysaccharide ratio	833:852	polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	833:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	1	20	theme	bran	150:153	arg1	constituents					200:211	the major active constituents	183:211	the major active constituents of rice bran (RB)	183:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	20	theme	bran	150:153	arg1	RBPSs					172:176	RBPSs	172:176	RBPSs	172:176	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	20	theme	bran	150:153	arg1	polysaccharides					155:169	Rice bran polysaccharides	145:169	Rice bran polysaccharides (RBPSs)	145:177	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	4	21	theme	test	613:616	arg1	results					586:592	the results	582:592	the results of a single-factor test	582:616	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	10	22	contain	contain	1650:1656	arg1	RBPSs					1643:1647	RBPSs	1643:1647	RBPSs	1643:1647	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	22	contain	contain	1650:1656	arg1	polysaccharides					1626:1640	PRACTICAL APPLICATION Rice bran polysaccharides	1594:1640	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs)	1594:1648	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	22	contain	contain	1650:1656	arg2	compounds					1658:1666	compounds	1658:1666	compounds with many biological activities	1658:1698	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	23	theme	PRACTICAL	1594:1602	arg1	RBPSs					1643:1647	RBPSs	1643:1647	RBPSs	1643:1647	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	23	theme	PRACTICAL	1594:1602	arg1	polysaccharides					1626:1640	PRACTICAL APPLICATION Rice bran polysaccharides	1594:1640	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs)	1594:1648	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	0	24	theme	Natural	86:92	arg1	Killer					94:99	Natural Killer	86:99	Natural Killer Cell Cytotoxicity	86:117	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	5	25	theme	reaction	864:871	arg1	temperature					873:883	a reaction temperature	862:883	a reaction temperature of 40 °C	862:892	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	8	26	theme	Da	1381:1382	arg1	RBPSs					1354:1358	the RBPSs	1350:1358	the RBPSs	1350:1358	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	11	27	theme	main	1824:1827	arg1	sites					1803:1807	unexposed active sites	1786:1807	unexposed active sites	1786:1807	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	27	theme	main	1824:1827	arg1	factors					1829:1835	the main factors	1820:1835	the main factors that limit their use in functional foods	1820:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	27	theme	main	1824:1827	arg1	weights					1774:1780	high molecular weights	1759:1780	high molecular weights	1759:1780	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	14	28	theme	beneficial	2167:2176	arg1	application					2178:2188	a new and beneficial application	2157:2188	a new and beneficial application for rice bran	2157:2202	The findings provide a new and beneficial application for rice bran.					
29939404	3	29	theme	natural	392:398	arg1	NK					408:409	NK	408:409	NK	408:409	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	3	29	theme	natural	392:398	arg1	killer					400:405	natural killer	392:405	natural killer (NK) cell cytotoxicity	392:428	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	0	30	theme	Cell	101:104	arg1	Cytotoxicity					106:117	Natural Killer Cell Cytotoxicity	86:117	Natural Killer Cell Cytotoxicity	86:117	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	14	31	theme	new	2159:2161	arg1	application					2178:2188	a new and beneficial application	2157:2188	a new and beneficial application for rice bran	2157:2202	The findings provide a new and beneficial application for rice bran.					
29939404	11	32	theme	unexposed	1786:1794	arg1	sites					1803:1807	unexposed active sites	1786:1807	unexposed active sites	1786:1807	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	32	theme	unexposed	1786:1794	arg1	factors					1829:1835	the main factors	1820:1835	the main factors that limit their use in functional foods	1820:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	11	32	theme	unexposed	1786:1794	arg1	weights					1774:1780	high molecular weights	1759:1780	high molecular weights	1759:1780	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	0	33	theme	Enzymatic	0:8	arg1	Modification					10:21	Enzymatic Modification	0:21	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa	0:83	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	13	34	theme	RBPSs	2114:2118	arg1	activities					2100:2109	immune and antioxidant activities	2077:2109	immune and antioxidant activities of RBPSs	2077:2118	In addition, immune and antioxidant activities of RBPSs were increased.					
29939404	4	35	theme	reaction	655:662	arg1	temperature					664:674	reaction temperature	655:674	reaction temperature	655:674	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	9	36	theme	Antioxidant	1438:1448	arg1	tests					1459:1463	Antioxidant activity tests	1438:1463	Antioxidant activity tests	1438:1463	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	0	37	theme	Bran	31:34	arg1	Polysaccharides					36:50	Rice Bran Polysaccharides	26:50	Rice Bran Polysaccharides	26:50	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	5	38	theme	pH	906:907	arg1	ratio					848:852	polysaccharide ratio	833:852	polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	833:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	3	39	theme	modification	548:559	arg1	conditions					561:570	the modification conditions	544:570	the modification conditions	544:570	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	3	40	theme	modified	433:440	arg1	mRBPSs					459:464	mRBPSs	459:464	mRBPSs	459:464	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	3	40	theme	modified	433:440	arg1	polysaccharides					442:456	modified polysaccharides	433:456	modified polysaccharides (mRBPSs) generated from RBPSs	433:486	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	5	41	theme	4	912:912	arg1	1:5					857:859	1:5	857:859	1:5	857:859	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	41	theme	4	912:912	arg1	pH					906:907	a reaction pH	895:907	a reaction pH of 4	895:912	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	41	theme	4	912:912	arg1	temperature					873:883	a reaction temperature	862:883	a reaction temperature of 40 °C	862:892	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	41	theme	4	912:912	arg1	time					930:933	a reaction time	919:933	a reaction time of 4 hr	919:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	9	42	theme	activity	1450:1457	arg1	tests					1459:1463	Antioxidant activity tests	1438:1463	Antioxidant activity tests	1438:1463	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	1	43	theme	rice	216:219	arg1	RB					227:228	RB	227:228	RB	227:228	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	43	theme	rice	216:219	arg1	bran					221:224	rice bran	216:224	rice bran (RB)	216:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	5	44	theme	°C	891:892	arg1	1:5					857:859	1:5	857:859	1:5	857:859	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	44	theme	°C	891:892	arg1	pH					906:907	a reaction pH	895:907	a reaction pH of 4	895:912	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	44	theme	°C	891:892	arg1	temperature					873:883	a reaction temperature	862:883	a reaction temperature of 40 °C	862:892	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	44	theme	°C	891:892	arg1	time					930:933	a reaction time	919:933	a reaction time of 4 hr	919:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	8	45	theme	High-performance	1271:1286	arg1	chromatography					1295:1308	High-performance liquid chromatography	1271:1308	High-performance liquid chromatography results	1271:1316	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	10	46	theme	bran	1621:1624	arg1	RBPSs					1643:1647	RBPSs	1643:1647	RBPSs	1643:1647	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	46	theme	bran	1621:1624	arg1	polysaccharides					1626:1640	PRACTICAL APPLICATION Rice bran polysaccharides	1594:1640	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs)	1594:1648	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	9	47	theme	mRBPSs6	1491:1497	arg1	capacity					1479:1486	high capacity	1474:1486	high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals	1474:1578	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	10	48	theme	APPLICATION	1604:1614	arg1	RBPSs					1643:1647	RBPSs	1643:1647	RBPSs	1643:1647	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	48	theme	APPLICATION	1604:1614	arg1	polysaccharides					1626:1640	PRACTICAL APPLICATION Rice bran polysaccharides	1594:1640	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs)	1594:1648	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	9	49	theme	scavenging	1503:1512	arg1	radicals					1544:1551	scavenging 1,1-diphenyl-2-picrylhydrazyl radicals	1503:1551	scavenging 1,1-diphenyl-2-picrylhydrazyl radicals	1503:1551	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	7	50	theme	chromatographic	1069:1083	arg1	Gas					1065:1067	Gas chromatographic	1065:1083	Gas chromatographic	1065:1083	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	11	51	from	use	1854:1856	arg1	foods					1872:1876	functional foods	1861:1876	functional foods	1861:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	9	52	theme	hydroxyl	1557:1564	arg1	radicals					1571:1578	hydroxyl free radicals	1557:1578	hydroxyl free radicals	1557:1578	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	12	53	theme	molecular	2005:2013	arg1	weights					2015:2021	the molecular weights	2001:2021	the molecular weights	2001:2021	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	10	54	theme	many	1673:1676	arg1	activities					1689:1698	many biological activities	1673:1698	many biological activities	1673:1698	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	5	55	theme	best	782:785	arg1	enzyme					823:828	an enzyme	820:828	an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	820:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	55	theme	best	782:785	arg1	conditions					787:796	The best conditions	778:796	The best conditions	778:796	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	4	56	theme	NK-cell	756:762	arg1	cytotoxicity					764:775	mRBPSs-enhanced NK-cell cytotoxicity	740:775	mRBPSs-enhanced NK-cell cytotoxicity	740:775	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	0	57	theme	Antioxidant	123:133	arg1	Activity					135:142	Antioxidant Activity	123:142	Antioxidant Activity	123:142	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	3	58	theme	orthogonal	492:501	arg1	test					503:506	an orthogonal test	489:506	an orthogonal test (L9 [3]4 )	489:517	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	3	58	theme	orthogonal	492:501	arg1	[3					512:513	L9 [3]4	509:515	L9 [3]4	509:515	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	1	59	theme	major	187:191	arg1	polysaccharides					155:169	Rice bran polysaccharides	145:169	Rice bran polysaccharides (RBPSs)	145:177	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	59	theme	major	187:191	arg1	constituents					200:211	the major active constituents	183:211	the major active constituents of rice bran (RB)	183:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	8	60	theme	chromatography	1295:1308	arg1	results					1310:1316	High-performance liquid chromatography results	1271:1316	High-performance liquid chromatography results	1271:1316	High-performance liquid chromatography results indicated molecular weights for the RBPSs of approximately 106 Da, which decreased to 104 to 105 Da after modification.					
29939404	12	61	theme	RBPSs	1938:1942	arg1	modification					1922:1933	modification	1922:1933	modification of RBPSs using intracellular enzymes from an edible fungus	1922:1992	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	12	62	theme	edible	1980:1985	arg1	fungus					1987:1992	an edible fungus	1977:1992	an edible fungus	1977:1992	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	7	63	theme	7:21:6:5:53:48	1209:1222	arg1	ratio					1200:1204	a molar ratio	1192:1204	a molar ratio	1192:1204	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	7	63	theme	7:21:6:5:53:48	1209:1222	arg1	8:13:8:5:44:44					1235:1248	8:13:8:5:44:44	1235:1248	8:13:8:5:44:44	1235:1248	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	5	64	theme	hr	940:941	arg1	1:5					857:859	1:5	857:859	1:5	857:859	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	64	theme	hr	940:941	arg1	pH					906:907	a reaction pH	895:907	a reaction pH of 4	895:912	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	64	theme	hr	940:941	arg1	temperature					873:883	a reaction temperature	862:883	a reaction temperature of 40 °C	862:892	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	5	64	theme	hr	940:941	arg1	time					930:933	a reaction time	919:933	a reaction time of 4 hr	919:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	6	65	theme	±	1056:1056	arg1	%					1062:1062	12.01% ± 0.08%	1049:1062	12.01% ± 0.08%	1049:1062	By optimizing the conditions, the NK-cell cytotoxicity induced by mRBPSs6 was the highest, increasing by 12.01% ± 0.08%.					
29939404	12	66	theme	intracellular	1950:1962	arg1	enzymes					1964:1970	intracellular enzymes	1950:1970	intracellular enzymes	1950:1970	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	11	67	theme	functional	1861:1870	arg1	foods					1872:1876	functional foods	1861:1876	functional foods	1861:1876	However, these polysaccharides difficult to absorb due to high molecular weights and unexposed active sites, which are the main factors that limit their use in functional foods.					
29939404	5	68	theme	reaction	921:928	arg1	time					930:933	a reaction time	919:933	a reaction time of 4 hr	919:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	1	69	theme	Rice	145:148	arg1	constituents					200:211	the major active constituents	183:211	the major active constituents of rice bran (RB)	183:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	69	theme	Rice	145:148	arg1	RBPSs					172:176	RBPSs	172:176	RBPSs	172:176	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	69	theme	Rice	145:148	arg1	polysaccharides					155:169	Rice bran polysaccharides	145:169	Rice bran polysaccharides (RBPSs)	145:177	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	10	70	with	compounds	1658:1666	arg1	activities					1689:1698	many biological activities	1673:1698	many biological activities	1673:1698	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	4	71	theme	single-factor	599:611	arg1	test					613:616	a single-factor test	597:616	a single-factor test	597:616	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	14	72	theme	rice	2194:2197	arg1	bran					2199:2202	rice bran	2194:2202	rice bran	2194:2202	The findings provide a new and beneficial application for rice bran.					
29939404	0	73	theme	Killer	94:99	arg1	Cytotoxicity					106:117	Natural Killer Cell Cytotoxicity	86:117	Natural Killer Cell Cytotoxicity	86:117	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	0	74	dep	Cytotoxicity	106:117	arg1	Modification					10:21	Enzymatic Modification	0:21	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa	0:83	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	5	75	theme	temperature	873:883	arg1	ratio					848:852	polysaccharide ratio	833:852	polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	833:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	4	76	theme	reaction	677:684	arg1	pH					686:687	reaction pH	677:687	reaction pH	677:687	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	3	77	theme	killer	400:405	arg1	cytotoxicity					417:428	natural killer (NK) cell cytotoxicity	392:428	natural killer (NK) cell cytotoxicity	392:428	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	4	78	theme	polysaccharide	633:646	arg1	ratio					648:652	polysaccharide ratio	633:652	polysaccharide ratio	633:652	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	0	79	from	Frondosa	76:83	arg1	Enzymes					55:61	Enzymes	55:61	Enzymes from Grifola Frondosa	55:83	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	0	80	theme	Rice	26:29	arg1	Polysaccharides					36:50	Rice Bran Polysaccharides	26:50	Rice Bran Polysaccharides	26:50	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	5	81	theme	1:5	857:859	arg1	ratio					848:852	polysaccharide ratio	833:852	polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr	833:941	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	7	82	from	ratio	1200:1204	arg1	of rhamnose					1125:1135	of rhamnose	1125:1135	of rhamnose	1125:1135	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	7	82	from	ratio	1200:1204	arg1	glucose					1166:1172	glucose	1166:1172	glucose	1166:1172	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	7	82	from	ratio	1200:1204	arg1	galactose					1179:1187	galactose	1179:1187	galactose	1179:1187	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	7	82	from	ratio	1200:1204	arg1	mannose					1157:1163	mannose	1157:1163	mannose	1157:1163	Gas chromatographic analysis revealed that mRBPSs6 consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 7:21:6:5:53:48, which was 8:13:8:5:44:44 before modification.					
29939404	3	83	theme	cell	412:415	arg1	cytotoxicity					417:428	natural killer (NK) cell cytotoxicity	392:428	natural killer (NK) cell cytotoxicity	392:428	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	0	84	theme	Polysaccharides	36:50	arg1	Modification					10:21	Enzymatic Modification	0:21	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa	0:83	Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity.					
29939404	5	85	theme	reaction	897:904	arg1	pH					906:907	a reaction pH	895:907	a reaction pH of 4	895:912	The best conditions were determined to be an enzyme to polysaccharide ratio of 1:5, a reaction temperature of 40 °C, a reaction pH of 4, and a reaction time of 4 hr.					
29939404	13	86	theme	antioxidant	2088:2098	arg1	activities					2100:2109	immune and antioxidant activities	2077:2109	immune and antioxidant activities of RBPSs	2077:2118	In addition, immune and antioxidant activities of RBPSs were increased.					
29939404	6	87	theme	NK-cell	978:984	arg1	cytotoxicity					986:997	the NK-cell cytotoxicity	974:997	the NK-cell cytotoxicity induced by mRBPSs6	974:1016	By optimizing the conditions, the NK-cell cytotoxicity induced by mRBPSs6 was the highest, increasing by 12.01% ± 0.08%.					
29939404	6	87	theme	NK-cell	978:984	arg1	highest					1026:1032	highest	1026:1032	highest	1026:1032	By optimizing the conditions, the NK-cell cytotoxicity induced by mRBPSs6 was the highest, increasing by 12.01% ± 0.08%.					
29939404	4	88	theme	main	717:720	arg1	enzyme					623:628	the enzyme	619:628	the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time	619:706	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	4	88	theme	main	717:720	arg1	factors					722:728	the main factors	713:728	the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity	713:775	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	3	89	theme	polysaccharides	442:456	arg1	effect					382:387	the effect	378:387	the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs	378:486	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	12	90	theme	RBPSs	2057:2061	arg1	weights					2015:2021	the molecular weights	2001:2021	the molecular weights	2001:2021	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	12	90	theme	RBPSs	2057:2061	arg1	composition					2042:2052	monosaccharide composition	2027:2052	monosaccharide composition of RBPSs	2027:2061	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	9	91	theme	high	1474:1477	arg1	capacity					1479:1486	high capacity	1474:1486	high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals	1474:1578	Antioxidant activity tests revealed high capacity of mRBPSs6 for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals and hydroxyl free radicals at 1.0 mg/mL.					
29939404	3	92	from	effect	382:387	arg1	cytotoxicity					417:428	natural killer (NK) cell cytotoxicity	392:428	natural killer (NK) cell cytotoxicity	392:428	To enhance the effect on natural killer (NK) cell cytotoxicity of modified polysaccharides (mRBPSs) generated from RBPSs, an orthogonal test (L9 [3]4 ) was employed to optimize the modification conditions.					
29939404	1	93	theme	bran	221:224	arg1	polysaccharides					155:169	Rice bran polysaccharides	145:169	Rice bran polysaccharides (RBPSs)	145:177	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	1	93	theme	bran	221:224	arg1	constituents					200:211	the major active constituents	183:211	the major active constituents of rice bran (RB)	183:229	Rice bran polysaccharides (RBPSs) are the major active constituents of rice bran (RB).					
29939404	12	94	theme	study	1899:1903	arg1	results					1883:1889	The results	1879:1889	The results of this study	1879:1903	The results of this study demonstrate that modification of RBPSs using intracellular enzymes from an edible fungus alters the molecular weights and monosaccharide composition of RBPSs.					
29939404	4	95	theme	reaction	694:701	arg1	time					703:706	reaction time	694:706	reaction time	694:706	Based on the results of a single-factor test, the enzyme to polysaccharide ratio, reaction temperature, reaction pH, and reaction time were the main factors affecting mRBPSs-enhanced NK-cell cytotoxicity.					
29939404	10	96	theme	Rice	1616:1619	arg1	RBPSs					1643:1647	RBPSs	1643:1647	RBPSs	1643:1647	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	10	96	theme	Rice	1616:1619	arg1	polysaccharides					1626:1640	PRACTICAL APPLICATION Rice bran polysaccharides	1594:1640	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs)	1594:1648	PRACTICAL APPLICATION Rice bran polysaccharides (RBPSs) contain compounds with many biological activities.					
29939404	13	97	theme	immune	2077:2082	arg1	activities					2100:2109	immune and antioxidant activities	2077:2109	immune and antioxidant activities of RBPSs	2077:2118	In addition, immune and antioxidant activities of RBPSs were increased.					
31730985	1	0	from	attention	207:215	arg1	years					227:231	recent years	220:231	recent years	220:231	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	7	1	theme	species	1113:1119	arg1	polysaccharide					1076:1089	the polysaccharide	1072:1089	the polysaccharide of the brackish water species	1072:1119	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	10	2	theme	applications	1500:1511	arg1	development					1474:1484	the development	1470:1484	the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery	1470:1580	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	7	3	theme	alkaline	1000:1007	arg1	environment					1009:1019	an alkaline environment	997:1019	an alkaline environment	997:1019	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	7	4	from	environment	1009:1019	arg1	stable					987:992	stable	987:992	stable	987:992	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	10	5	theme	innovative	1489:1498	arg1	applications					1500:1511	innovative applications	1489:1511	innovative applications for the biotech industries for pharmaceutics, food and drug-delivery	1489:1580	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	3	6	theme	polysaccharides	578:592	arg1	study					538:542	a comparative study	524:542	a comparative study of the two less explored sulfated polysaccharides	524:592	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	8	7	theme	similar	1196:1202	arg1	morphology					1204:1213	a similar morphology	1194:1213	a similar morphology	1194:1213	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	6	8	theme	gel	951:953	arg1	behavior					955:962	weak gel behavior	946:962	weak gel behavior	946:962	Solutions of both polysaccharides were highly viscous and exhibited shear thinning and weak gel behavior.					
31730985	7	9	theme	freshwater	1149:1158	arg1	species					1160:1166	the freshwater species	1145:1166	the freshwater species	1145:1166	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	7	10	from	stable	987:992	arg1	environment					1009:1019	an alkaline environment	997:1019	an alkaline environment	997:1019	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	6	11	theme	weak	946:949	arg1	behavior					955:962	weak gel behavior	946:962	weak gel behavior	946:962	Solutions of both polysaccharides were highly viscous and exhibited shear thinning and weak gel behavior.					
31730985	0	12	dep	Porphyridium	109:120	arg1	aerugineum					122:131	Porphyridium aerugineum	109:131	Porphyridium aerugineum	109:131	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	1	13	theme	increasing	196:205	arg1	attention					207:215	increasing attention	196:215	increasing attention in recent years	196:231	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	8	14	theme	rough	1257:1261	arg1	surface					1273:1279	a rough amorphous surface	1255:1279	a rough amorphous surface	1255:1279	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	10	15	theme	sound	1378:1382	arg1	basis					1384:1388	a sound basis	1376:1388	a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery	1376:1580	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	0	16	theme	Physico-chemical	0:15	arg1	Dixoniella					87:96	Dixoniella	87:96	Dixoniella	87:96	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	16	theme	Physico-chemical	0:15	arg1	Porphyridium					109:120	Porphyridium	109:120	Porphyridium	109:120	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	16	theme	Physico-chemical	0:15	arg1	characteristics					17:31	Physico-chemical characteristics	0:31	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.	0:132	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	2	17	theme	Porphyridium	476:487	arg1	aerugineum					489:498	the freshwater- Porphyridium aerugineum	460:498	the freshwater- Porphyridium aerugineum	460:498	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	9	18	from	studies	1308:1314	arg1	sp					1336:1337	the Porphyridium sp	1319:1337	the Porphyridium sp	1319:1337	By complementing previous studies on the Porphyridium sp.					
31730985	10	19	theme	biotech	1521:1527	arg1	industries					1529:1538	the biotech industries	1517:1538	the biotech industries for pharmaceutics, food and drug-delivery	1517:1580	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	2	20	theme	freshwater-	464:474	arg1	aerugineum					489:498	the freshwater- Porphyridium aerugineum	460:498	the freshwater- Porphyridium aerugineum	460:498	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	3	21	from	differences	630:640	arg1	composition					645:655	composition	645:655	composition	645:655	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	3	21	from	differences	630:640	arg1	properties					667:676	physical properties	658:676	physical properties	658:676	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	3	21	from	differences	630:640	arg1	biocompatibility					682:697	biocompatibility	682:697	biocompatibility	682:697	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	1	22	theme	recent	220:225	arg1	years					227:231	recent years	220:231	recent years	220:231	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	3	23	theme	physical	658:665	arg1	properties					667:676	physical properties	658:676	physical properties	658:676	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	7	24	theme	water	1107:1111	arg1	species					1113:1119	the brackish water species	1094:1119	the brackish water species	1094:1119	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	8	25	theme	fibrous	1232:1238	arg1	structure					1240:1248	a porous fibrous structure	1223:1248	a porous fibrous structure with a rough amorphous surface	1223:1279	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	3	26	theme	explored	560:567	arg1	polysaccharides					578:592	the two less explored sulfated polysaccharides	547:592	the two less explored sulfated polysaccharides	547:592	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	6	27	theme	shear	927:931	arg1	thinning					933:940	shear thinning	927:940	shear thinning	927:940	Solutions of both polysaccharides were highly viscous and exhibited shear thinning and weak gel behavior.					
31730985	2	28	dep	Porphyridium	361:372	arg1	sp.					374:376	sp.	374:376	sp.	374:376	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	2	29	theme	Dixoniella	438:447	arg1	grisea					449:454	the brackish water- Dixoniella grisea	418:454	the brackish water- Dixoniella grisea	418:454	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	0	30	theme	polysaccharides	49:63	arg1	Dixoniella					87:96	Dixoniella	87:96	Dixoniella	87:96	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	30	theme	polysaccharides	49:63	arg1	Porphyridium					109:120	Porphyridium	109:120	Porphyridium	109:120	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	30	theme	polysaccharides	49:63	arg1	characteristics					17:31	Physico-chemical characteristics	0:31	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.	0:132	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	2	31	theme	Porphyridium	361:372	arg1	species					353:359	the marine species	342:359	the marine species Porphyridium sp.	342:376	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	2	32	theme	water-	431:436	arg1	grisea					449:454	the brackish water- Dixoniella grisea	418:454	the brackish water- Dixoniella grisea	418:454	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	0	33	theme	sulfated	40:47	arg1	polysaccharides					49:63	the sulfated polysaccharides	36:63	the sulfated polysaccharides of the red microalgae	36:85	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	5	34	theme	Sulfur	793:798	arg1	content					800:806	Sulfur content	793:806	Sulfur content	793:806	Sulfur content was 0.8% for P. aerugineum and 1.6% for D. grisea.					
31730985	8	35	with	structure	1240:1248	arg1	surface					1273:1279	a rough amorphous surface	1255:1279	a rough amorphous surface	1255:1279	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	2	36	theme	brackish	422:429	arg1	grisea					449:454	the brackish water- Dixoniella grisea	418:454	the brackish water- Dixoniella grisea	418:454	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	8	37	theme	amorphous	1263:1271	arg1	surface					1273:1279	a rough amorphous surface	1255:1279	a rough amorphous surface	1255:1279	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	7	38	theme	polysaccharide	1076:1089	arg1	viscosity					1059:1067	the viscosity	1055:1067	the viscosity of the polysaccharide of the brackish water species	1055:1119	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	3	39	theme	comparative	526:536	arg1	study					538:542	a comparative study	524:542	a comparative study of the two less explored sulfated polysaccharides	524:592	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	7	40	theme	brackish	1098:1105	arg1	species					1113:1119	the brackish water species	1094:1119	the brackish water species	1094:1119	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	9	41	theme	previous	1299:1306	arg1	studies					1308:1314	previous studies	1299:1314	previous studies on the Porphyridium sp	1299:1337	By complementing previous studies on the Porphyridium sp.					
31730985	1	42	theme	microalgae	170:179	arg1	polysaccharides					147:161	The sulfated polysaccharides	134:161	The sulfated polysaccharides of red microalgae	134:179	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	7	43	theme	acidic	1036:1041	arg1	environment					1043:1053	an acidic environment	1033:1053	an acidic environment	1033:1053	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	1	44	theme	sulfated	138:145	arg1	polysaccharides					147:161	The sulfated polysaccharides	134:161	The sulfated polysaccharides of red microalgae	134:179	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	10	45	dep	promote	1462:1468	arg1	will					1457:1460	will	1457:1460	will	1457:1460	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	8	46	theme	porous	1225:1230	arg1	structure					1240:1248	a porous fibrous structure	1223:1248	a porous fibrous structure with a rough amorphous surface	1223:1279	Both exhibited a similar morphology, having a porous fibrous structure with a rough amorphous surface.					
31730985	2	47	theme	most	292:295	arg1	studies					297:303	most studies	292:303	most studies	292:303	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	2	48	theme	species	353:359	arg1	polysaccharide					324:337	the polysaccharide	320:337	the polysaccharide of the marine species Porphyridium sp.	320:376	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	3	49	theme	sulfated	569:576	arg1	polysaccharides					578:592	the two less explored sulfated polysaccharides	547:592	the two less explored sulfated polysaccharides	547:592	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	6	50	theme	polysaccharides	877:891	arg1	Solutions					859:867	Solutions	859:867	Solutions of both polysaccharides	859:891	Solutions of both polysaccharides were highly viscous and exhibited shear thinning and weak gel behavior.					
31730985	2	51	theme	marine	346:351	arg1	species					353:359	the marine species	342:359	the marine species Porphyridium sp.	342:376	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	3	52	from	similarities	613:624	arg1	composition					645:655	composition	645:655	composition	645:655	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	3	52	from	similarities	613:624	arg1	properties					667:676	physical properties	658:676	physical properties	658:676	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	3	52	from	similarities	613:624	arg1	biocompatibility					682:697	biocompatibility	682:697	biocompatibility	682:697	We therefore conducted a comparative study of the two less explored sulfated polysaccharides, emphasizing their similarities and differences in composition, physical properties and biocompatibility.					
31730985	0	53	theme	microalgae	76:85	arg1	polysaccharides					49:63	the sulfated polysaccharides	36:63	the sulfated polysaccharides of the red microalgae	36:85	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	54	dep	characteristics	17:31	arg1	Dixoniella					87:96	Dixoniella	87:96	Dixoniella	87:96	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	54	dep	characteristics	17:31	arg1	Porphyridium					109:120	Porphyridium	109:120	Porphyridium	109:120	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	0	54	dep	characteristics	17:31	arg1	characteristics					17:31	Physico-chemical characteristics	0:31	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.	0:132	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	10	55	theme	structure/function	1410:1427	arg1	relationships					1429:1441	the structure/function relationships	1406:1441	the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery	1406:1580	polysaccharide, we have established a sound basis for elucidating the structure/function relationships that in turn, will promote the development of innovative applications for the biotech industries for pharmaceutics, food and drug-delivery.					
31730985	1	56	theme	red	166:168	arg1	microalgae					170:179	red microalgae	166:179	red microalgae	166:179	The sulfated polysaccharides of red microalgae have attracted increasing attention in recent years due to their unique rheological and bioactivities.					
31730985	0	57	theme	red	72:74	arg1	microalgae					76:85	the red microalgae	68:85	the red microalgae	68:85	Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum.					
31730985	9	58	theme	Porphyridium	1323:1334	arg1	sp					1336:1337	the Porphyridium sp	1319:1337	the Porphyridium sp	1319:1337	By complementing previous studies on the Porphyridium sp.					
31730985	2	59	theme	limited	384:390	arg1	information					392:402	limited information	384:402	limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum	384:498	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730985	7	60	dep	found	975:979	arg1	whereas					1022:1028	whereas	1022:1028	whereas	1022:1028	Both were found to be stable in an alkaline environment, whereas in an acidic environment the viscosity of the polysaccharide of the brackish water species increased while that of the freshwater species decreased.					
31730985	2	61	with	devoted	309:315	arg1	information					392:402	limited information	384:402	limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum	384:498	Todate, most studies are devoted to the polysaccharide of the marine species Porphyridium sp., with limited information about that of the brackish water- Dixoniella grisea and the freshwater- Porphyridium aerugineum.					
31730990	5	0	theme	tumor	776:780	arg1	growth					792:797	tumor xenograft growth	776:797	tumor xenograft growth	776:797	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	4	1	theme	[→	539:540	arg1	2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→					571:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	5	2	theme	xenograft	782:790	arg1	growth					792:797	tumor xenograft growth	776:797	tumor xenograft growth	776:797	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	5	3	dep	cell	829:832	arg1	expansion					834:842	expansion	834:842	CD4+ T cell expansion	822:842	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	3	4	theme	glucose	471:477	arg1	weight					357:362	The average molecular weight	335:362	The average molecular weight of BOP	335:369	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	4	theme	glucose	471:477	arg1	composition					426:436	the monosaccharide composition	407:436	the monosaccharide composition	407:436	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	4	theme	glucose	471:477	arg1	Da					399:400	approximately 4.9 × 105 Da	375:400	approximately 4.9 × 105 Da	375:400	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	4	theme	glucose	471:477	arg1	mix					452:454	7.88:2.12 mix	442:454	7.88:2.12 mix of mannose and glucose	442:477	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	5	5	theme	carcinoma	732:740	arg1	cells					742:746	CT26 colon carcinoma cells	721:746	CT26 colon carcinoma cells	721:746	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	4	6	theme	4-β-D-Man-1	542:552	arg1	2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→					571:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	5	7	theme	cells	742:746	arg1	rate					713:716	the proliferation rate	695:716	the proliferation rate of CT26 colon carcinoma cells	695:746	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	4	8	dep	linkage	530:536	arg1	2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→					571:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	2	9	theme	BOP	273:275	arg1	features					261:268	The structural features	246:268	The structural features of BOP	246:275	The structural features of BOP were analyzed by GC-MS, HPLP, FT-IR, methylation and NMR.					
31730990	0	10	theme	polysaccharide	39:52	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and anti-tumor activity of a polysaccharide from Bletilla ochracea Schltr.					
31730990	0	10	theme	polysaccharide	39:52	arg1	activity					25:32	anti-tumor activity	14:32	anti-tumor activity	14:32	Structure and anti-tumor activity of a polysaccharide from Bletilla ochracea Schltr.					
31730990	5	11	contain	had	678:680	arg1	BOP					674:676	BOP	674:676	BOP	674:676	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	5	11	contain	had	678:680	arg2	effect					685:690	no effect	682:690	no effect	682:690	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	3	12	theme	molecular	347:355	arg1	weight					357:362	The average molecular weight	335:362	The average molecular weight of BOP	335:369	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	12	theme	molecular	347:355	arg1	Da					399:400	approximately 4.9 × 105 Da	375:400	approximately 4.9 × 105 Da	375:400	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	1	13	attach	isolated	144:151	arg2	fraction					104:111	The polysaccharide fraction	85:111	The polysaccharide fraction of Bletilla ochracea (BOP)	85:138	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	1	13	attach	isolated	144:151	arg1	tubers					162:167	its tubers	158:167	its tubers	158:167	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	5	14	theme	tumor-bearing	865:877	arg1	mice					879:882	the tumor-bearing mice	861:882	the tumor-bearing mice	861:882	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	5	15	theme	CD4+	822:825	arg1	cell					829:832	CD4+ T cell expansion	822:842	CD4+ T cell expansion	822:842	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	6	16	theme	anti-tumor	967:976	arg1	therapy					978:984	anti-tumor therapy	967:984	anti-tumor therapy	967:984	Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.					
31730990	6	17	theme	immunomodulatory	917:932	arg1	agent					934:938	a potent immunomodulatory agent	908:938	a potent immunomodulatory agent that can be considered for anti-tumor therapy	908:984	Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.					
31730990	6	17	theme	immunomodulatory	917:932	arg1	BOP					901:903	BOP	901:903	BOP	901:903	Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.					
31730990	1	18	theme	polysaccharide	89:102	arg1	fraction					104:111	The polysaccharide fraction	85:111	The polysaccharide fraction of Bletilla ochracea (BOP)	85:138	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	5	19	theme	mice	879:882	arg1	spleen					851:856	the spleen	847:856	the spleen of the tumor-bearing mice	847:882	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	5	20	theme	T	827:827	arg1	cell					829:832	CD4+ T cell expansion	822:842	CD4+ T cell expansion	822:842	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	4	21	theme	following	520:528	arg1	linkage					530:536	the following linkage	516:536	the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→]	516:662	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	3	22	theme	average	339:345	arg1	weight					357:362	The average molecular weight	335:362	The average molecular weight of BOP	335:369	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	22	theme	average	339:345	arg1	Da					399:400	approximately 4.9 × 105 Da	375:400	approximately 4.9 × 105 Da	375:400	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	23	theme	mannose	459:465	arg1	weight					357:362	The average molecular weight	335:362	The average molecular weight of BOP	335:369	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	23	theme	mannose	459:465	arg1	composition					426:436	the monosaccharide composition	407:436	the monosaccharide composition	407:436	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	23	theme	mannose	459:465	arg1	Da					399:400	approximately 4.9 × 105 Da	375:400	approximately 4.9 × 105 Da	375:400	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	23	theme	mannose	459:465	arg1	mix					452:454	7.88:2.12 mix	442:454	7.88:2.12 mix of mannose and glucose	442:477	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	5	24	theme	CT26	721:724	arg1	cells					742:746	CT26 colon carcinoma cells	721:746	CT26 colon carcinoma cells	721:746	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	0	25	from	Schltr	77:82	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and anti-tumor activity of a polysaccharide from Bletilla ochracea Schltr.					
31730990	0	25	from	Schltr	77:82	arg1	activity					25:32	anti-tumor activity	14:32	anti-tumor activity	14:32	Structure and anti-tumor activity of a polysaccharide from Bletilla ochracea Schltr.					
31730990	1	26	theme	DEAE-52	186:192	arg1	Cellulose					194:202	DEAE-52 Cellulose and Sephadex G-200 column chromatography	186:243	Cellulose	194:202	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	5	27	theme	colon	726:730	arg1	cells					742:746	CT26 colon carcinoma cells	721:746	CT26 colon carcinoma cells	721:746	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	4	28	theme	4-β-D-Glc-1	559:569	arg1	2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→					571:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	3	29	theme	monosaccharide	411:424	arg1	composition					426:436	the monosaccharide composition	407:436	the monosaccharide composition	407:436	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	29	theme	monosaccharide	411:424	arg1	mix					452:454	7.88:2.12 mix	442:454	7.88:2.12 mix of mannose and glucose	442:477	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	4	30	theme	FT-IR	484:488	arg1	analyses					498:505	The FT-IR and NMR analyses	480:505	analyses	498:505	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	1	31	theme	Bletilla	116:123	arg1	BOP					135:137	BOP	135:137	BOP	135:137	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	1	31	theme	Bletilla	116:123	arg1	ochracea					125:132	Bletilla ochracea	116:132	Bletilla ochracea (BOP)	116:138	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	3	32	theme	BOP	367:369	arg1	weight					357:362	The average molecular weight	335:362	The average molecular weight of BOP	335:369	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	32	theme	BOP	367:369	arg1	Da					399:400	approximately 4.9 × 105 Da	375:400	approximately 4.9 × 105 Da	375:400	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	1	33	theme	ochracea	125:132	arg1	fraction					104:111	The polysaccharide fraction	85:111	The polysaccharide fraction of Bletilla ochracea (BOP)	85:138	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	4	34	theme	NMR	494:496	arg1	analyses					498:505	The FT-IR and NMR analyses	480:505	analyses	498:505	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	2	35	theme	structural	250:259	arg1	features					261:268	The structural features	246:268	The structural features of BOP	246:275	The structural features of BOP were analyzed by GC-MS, HPLP, FT-IR, methylation and NMR.					
31730990	3	36	theme	7.88:2.12	442:450	arg1	composition					426:436	the monosaccharide composition	407:436	the monosaccharide composition	407:436	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	3	36	theme	7.88:2.12	442:450	arg1	mix					452:454	7.88:2.12 mix	442:454	7.88:2.12 mix of mannose and glucose	442:477	The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose.					
31730990	4	37	theme	4 → 	554:557	arg1	2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→					571:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	[→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→	539:661	The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→].					
31730990	5	38	theme	proliferation	699:711	arg1	rate					713:716	the proliferation rate	695:716	the proliferation rate of CT26 colon carcinoma cells	695:746	Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice.					
31730990	1	39	theme	Sephadex	208:215	arg1	chromatography					230:243	DEAE-52 Cellulose and Sephadex G-200 column chromatography	186:243	chromatography	230:243	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	1	40	theme	G-200	217:221	arg1	chromatography					230:243	DEAE-52 Cellulose and Sephadex G-200 column chromatography	186:243	chromatography	230:243	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31730990	6	41	theme	potent	910:915	arg1	agent					934:938	a potent immunomodulatory agent	908:938	a potent immunomodulatory agent that can be considered for anti-tumor therapy	908:984	Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.					
31730990	6	41	theme	potent	910:915	arg1	BOP					901:903	BOP	901:903	BOP	901:903	Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.					
31730990	0	42	theme	anti-tumor	14:23	arg1	activity					25:32	anti-tumor activity	14:32	anti-tumor activity	14:32	Structure and anti-tumor activity of a polysaccharide from Bletilla ochracea Schltr.					
31730990	1	43	theme	column	223:228	arg1	chromatography					230:243	DEAE-52 Cellulose and Sephadex G-200 column chromatography	186:243	chromatography	230:243	The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography.					
31116962	0	0	theme	tree	77:80	arg1	parts					58:62	different parts	48:62	different parts of date palm tree (Phoenix dactylifera L.)	48:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	4	1	theme	hemicellulose	769:781	arg1	content					747:753	the content	743:753	the content of cellulose, hemicellulose and lignin	743:792	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	2	2	theme	different	323:331	arg1	Phoenix					349:355	Phoenix	349:355	Phoenix	349:355	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	2	2	theme	different	323:331	arg1	fibers					373:378	different date palm tree (Phoenix dactylifera L.) fibers	323:378	different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method	323:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	5	3	from	fibers	863:868	arg1	calcium					815:821	calcium	815:821	calcium	815:821	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	3	from	fibers	863:868	arg1	high					837:840	high	837:840	high	837:840	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	3	from	fibers	863:868	arg1	amount					805:810	the amount	801:810	the amount of calcium	801:821	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	1	4	theme	materials	236:244	arg1	consumption					209:219	consumption	209:219	consumption	209:219	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	1	4	theme	materials	236:244	arg1	utilization					193:203	utilization	193:203	utilization	193:203	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	0	5	theme	palm	72:75	arg1	tree					77:80	date palm tree	67:80	date palm tree (Phoenix dactylifera L.)	67:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	0	5	theme	palm	72:75	arg1	L					103:103	Phoenix dactylifera L.	83:104	Phoenix dactylifera L.	83:104	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	3	6	theme	stalk	604:608	arg1	fibers					618:623	fruit bunch stalk (DPFBS) fibers	592:623	fruit bunch stalk (DPFBS) fibers	592:623	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	6	7	theme	DPLSH	1097:1101	arg1	samples					1103:1109	DPLSH samples	1097:1109	DPLSH samples	1097:1109	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	8	8	theme	biomass	1367:1373	arg1	material					1375:1382	biomass material	1367:1382	biomass material	1367:1382	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	0	9	theme	Phoenix	83:89	arg1	tree					77:80	date palm tree	67:80	date palm tree (Phoenix dactylifera L.)	67:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	0	9	theme	Phoenix	83:89	arg1	L					103:103	Phoenix dactylifera L.	83:104	Phoenix dactylifera L.	83:104	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	6	10	theme	thermal	1049:1055	arg1	DPFBS					1002:1006	DPFBS	1002:1006	DPFBS	1002:1006	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	10	theme	thermal	1049:1055	arg1	DPLST					992:996	DPLST	992:996	DPLST	992:996	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	10	theme	thermal	1049:1055	arg1	temperature					1069:1079	higher thermal degradation temperature	1042:1079	higher thermal degradation temperature	1042:1079	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	4	11	theme	lignin	787:792	arg1	content					747:753	the content	743:753	the content of cellulose, hemicellulose and lignin	743:792	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	3	12	theme	thermal	477:483	arg1	characterization					503:518	thermal and morphological characterization	477:518	thermal and morphological characterization of trunk (DPTRF)	477:535	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	6	13	theme	degradation	1057:1067	arg1	DPFBS					1002:1006	DPFBS	1002:1006	DPFBS	1002:1006	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	13	theme	degradation	1057:1067	arg1	DPLST					992:996	DPLST	992:996	DPLST	992:996	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	13	theme	degradation	1057:1067	arg1	temperature					1069:1079	higher thermal degradation temperature	1042:1079	higher thermal degradation temperature	1042:1079	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	4	14	theme	Chemical	642:649	arg1	analysis					651:658	Chemical analysis	642:658	Chemical analysis	642:658	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	5	15	theme	date	853:856	arg1	fibers					863:868	all the date palm fibers	845:868	all the date palm fibers	845:868	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	3	16	theme	trunk	523:527	arg1	DPLST					550:554	DPLST	550:554	DPLST	550:554	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	sheath					573:578	leaf sheath	568:578	leaf sheath	568:578	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	DPLSH					581:585	DPLSH	581:585	DPLSH	581:585	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	sheath					558:563	sheath	558:563	sheath	558:563	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	crystallinity					462:474	crystallinity	462:474	crystallinity	462:474	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	stalk					543:547	leaf stalk	538:547	leaf stalk (DPLST)	538:555	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	fibers					618:623	fruit bunch stalk (DPFBS) fibers	592:623	fruit bunch stalk (DPFBS) fibers	592:623	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	16	theme	trunk	523:527	arg1	characterization					503:518	thermal and morphological characterization	477:518	thermal and morphological characterization of trunk (DPTRF)	477:535	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	17	theme	morphological	489:501	arg1	characterization					503:518	thermal and morphological characterization	477:518	thermal and morphological characterization of trunk (DPTRF)	477:535	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	7	18	theme	DPFBS	1173:1177	arg1	surface					1162:1168	the fracture surface	1149:1168	the fracture surface of DPFBS	1149:1177	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	7	18	theme	DPFBS	1173:1177	arg1	rougher					1194:1200	rougher	1194:1200	rougher	1194:1200	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	0	19	theme	dactylifera	91:101	arg1	tree					77:80	date palm tree	67:80	date palm tree (Phoenix dactylifera L.)	67:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	0	19	theme	dactylifera	91:101	arg1	L					103:103	Phoenix dactylifera L.	83:104	Phoenix dactylifera L.	83:104	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	5	20	theme	72.4	961:964	arg1	%					965:965	%	965:965	%	965:965	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	6	21	dep	DPLST	992:996	arg1	fibers					1008:1013	fibers	1008:1013	fibers	1008:1013	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	2	22	theme	water	415:419	arg1	method					429:434	the conventional water retting method	398:434	the conventional water retting method	398:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	5	23	theme	%	965:965	arg1	crystallinity					967:979	72.4% crystallinity	961:979	72.4% crystallinity	961:979	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	4	24	theme	cellulose	758:766	arg1	content					747:753	the content	743:753	the content of cellulose, hemicellulose and lignin	743:792	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	3	25	dep	crystallinity	462:474	arg1	The					437:439	The	437:439	The	437:439	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	8	26	theme	polymer	1494:1500	arg1	composites					1502:1511	polymer composites	1494:1511	polymer composites	1494:1511	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	1	27	theme	strict	146:151	arg1	regulations					167:177	the strict environmental regulations	142:177	the strict environmental regulations regarding the utilization and consumption of ecofriendly materials	142:244	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	2	28	theme	conventional	402:413	arg1	method					429:434	the conventional water retting method	398:434	the conventional water retting method	398:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	8	29	theme	cellulose	1416:1424	arg1	nanofibers					1426:1435	respective cellulose nanofibers	1405:1435	respective cellulose nanofibers	1405:1435	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	2	30	theme	study	280:284	arg1	aim					268:270	the aim	264:270	the aim of this study	264:284	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	2	31	theme	tree	343:346	arg1	Phoenix					349:355	Phoenix	349:355	Phoenix	349:355	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	2	31	theme	tree	343:346	arg1	fibers					373:378	different date palm tree (Phoenix dactylifera L.) fibers	323:378	different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method	323:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	0	32	theme	fiber	28:32	arg1	Characterization					0:15	Characterization	0:15	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).	0:106	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	6	33	theme	higher	1042:1047	arg1	DPFBS					1002:1006	DPFBS	1002:1006	DPFBS	1002:1006	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	33	theme	higher	1042:1047	arg1	DPLST					992:996	DPLST	992:996	DPLST	992:996	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	33	theme	higher	1042:1047	arg1	temperature					1069:1079	higher thermal degradation temperature	1042:1079	higher thermal degradation temperature	1042:1079	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	7	34	theme	increased	1232:1240	arg1	strength					1250:1257	increased bonding strength	1232:1257	increased bonding strength with polymers in composites	1232:1285	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	7	35	with	strength	1250:1257	arg1	polymers					1264:1271	polymers	1264:1271	polymers in composites	1264:1285	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	0	36	theme	natural	20:26	arg1	fiber					28:32	natural fiber	20:32	natural fiber	20:32	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	5	37	theme	palm	858:861	arg1	fibers					863:868	all the date palm fibers	845:868	all the date palm fibers	845:868	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	38	theme	69.2	901:904	arg1	%					905:905	%	905:905	%	905:905	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	1	39	theme	current	112:118	arg1	study					120:124	The current study	108:124	The current study	108:124	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	8	40	theme	nanofibers	1426:1435	arg1	isolation					1392:1400	the isolation	1388:1400	the isolation of respective cellulose nanofibers and cellulose nanocrystals	1388:1462	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	4	41	theme	noteworthy	717:726	arg1	differences					728:738	noteworthy differences	717:738	noteworthy differences in the content of cellulose, hemicellulose and lignin	717:792	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	1	42	theme	environmental	153:165	arg1	regulations					167:177	the strict environmental regulations	142:177	the strict environmental regulations regarding the utilization and consumption of ecofriendly materials	142:244	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	6	43	dep	stable	1034:1039	arg1	DPFBS					1002:1006	DPFBS	1002:1006	DPFBS	1002:1006	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	43	dep	stable	1034:1039	arg1	DPLST					992:996	DPLST	992:996	DPLST	992:996	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	6	43	dep	stable	1034:1039	arg1	temperature					1069:1079	higher thermal degradation temperature	1042:1079	higher thermal degradation temperature	1042:1079	Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples.					
31116962	8	44	theme	nanocrystals	1451:1462	arg1	isolation					1392:1400	the isolation	1388:1400	the isolation of respective cellulose nanofibers and cellulose nanocrystals	1388:1462	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	8	45	from	reinforcement	1477:1489	arg1	composites					1502:1511	polymer composites	1494:1511	polymer composites	1494:1511	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	3	46	theme	leaf	568:571	arg1	sheath					573:578	leaf sheath	568:578	leaf sheath	568:578	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	7	47	theme	bonding	1242:1248	arg1	strength					1250:1257	increased bonding strength	1232:1257	increased bonding strength with polymers in composites	1232:1285	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	7	48	theme	fracture	1153:1160	arg1	surface					1162:1168	the fracture surface	1149:1168	the fracture surface of DPFBS	1149:1177	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	7	48	theme	fracture	1153:1160	arg1	rougher					1194:1200	rougher	1194:1200	rougher	1194:1200	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	8	49	theme	cellulose	1441:1449	arg1	nanocrystals					1451:1462	cellulose nanocrystals	1441:1462	cellulose nanocrystals	1441:1462	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	8	50	theme	respective	1405:1414	arg1	nanofibers					1426:1435	respective cellulose nanofibers	1405:1435	respective cellulose nanofibers	1405:1435	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	3	51	theme	leaf	538:541	arg1	DPLST					550:554	DPLST	550:554	DPLST	550:554	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	51	theme	leaf	538:541	arg1	stalk					543:547	leaf stalk	538:547	leaf stalk (DPLST)	538:555	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	4	52	from	differences	728:738	arg1	content					747:753	the content	743:753	the content of cellulose, hemicellulose and lignin	743:792	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	3	53	theme	elemental	451:459	arg1	DPLSH					581:585	DPLSH	581:585	DPLSH	581:585	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	53	theme	elemental	451:459	arg1	crystallinity					462:474	crystallinity	462:474	crystallinity	462:474	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	0	54	theme	different	48:56	arg1	parts					58:62	different parts	48:62	different parts of date palm tree (Phoenix dactylifera L.)	48:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	8	55	theme	potential	1467:1475	arg1	reinforcement					1477:1489	potential reinforcement	1467:1489	potential reinforcement in polymer composites	1467:1511	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	2	56	theme	retting	421:427	arg1	method					429:434	the conventional water retting method	398:434	the conventional water retting method	398:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	5	57	theme	%	905:905	arg1	crystallinity					907:919	69.2% crystallinity	901:919	69.2% crystallinity	901:919	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	4	58	theme	palm	697:700	arg1	fibers					702:707	date palm fibers	692:707	date palm fibers	692:707	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	3	59	theme	bunch	598:602	arg1	fibers					618:623	fruit bunch stalk (DPFBS) fibers	592:623	fruit bunch stalk (DPFBS) fibers	592:623	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	4	60	theme	date	692:695	arg1	fibers					702:707	date palm fibers	692:707	date palm fibers	692:707	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	3	61	theme	chemical	441:448	arg1	DPLSH					581:585	DPLSH	581:585	DPLSH	581:585	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	61	theme	chemical	441:448	arg1	crystallinity					462:474	crystallinity	462:474	crystallinity	462:474	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	7	62	from	polymers	1264:1271	arg1	composites					1276:1285	composites	1276:1285	composites	1276:1285	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	8	63	theme	alternative	1339:1349	arg1	sustainable					1351:1361	promising alternative sustainable	1329:1361	promising alternative sustainable	1329:1361	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	1	64	dep	utilization	193:203	arg1	the					189:191	the	189:191	the	189:191	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31116962	5	65	with	DPLSH	950:954	arg1	crystallinity					967:979	72.4% crystallinity	961:979	72.4% crystallinity	961:979	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	66	theme	calcium	815:821	arg1	calcium					815:821	calcium	815:821	calcium	815:821	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	66	theme	calcium	815:821	arg1	high					837:840	high	837:840	high	837:840	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	5	66	theme	calcium	815:821	arg1	amount					805:810	the amount	801:810	the amount of calcium	801:821	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	0	67	theme	date	67:70	arg1	tree					77:80	date palm tree	67:80	date palm tree (Phoenix dactylifera L.)	67:105	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	0	67	theme	date	67:70	arg1	L					103:103	Phoenix dactylifera L.	83:104	Phoenix dactylifera L.	83:104	Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).					
31116962	8	68	theme	promising	1329:1337	arg1	sustainable					1351:1361	promising alternative sustainable	1329:1361	promising alternative sustainable	1329:1361	Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.					
31116962	4	69	theme	fibers	702:707	arg1	types					683:687	the four types	674:687	the four types of date palm fibers	674:707	Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin.					
31116962	2	70	theme	palm	338:341	arg1	Phoenix					349:355	Phoenix	349:355	Phoenix	349:355	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	2	70	theme	palm	338:341	arg1	fibers					373:378	different date palm tree (Phoenix dactylifera L.) fibers	323:378	different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method	323:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	5	71	from	high	837:840	arg1	fibers					863:868	all the date palm fibers	845:868	all the date palm fibers	845:868	Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity.					
31116962	3	72	theme	fruit	592:596	arg1	fibers					618:623	fruit bunch stalk (DPFBS) fibers	592:623	fruit bunch stalk (DPFBS) fibers	592:623	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	3	73	theme	DPFBS	611:615	arg1	fibers					618:623	fruit bunch stalk (DPFBS) fibers	592:623	fruit bunch stalk (DPFBS) fibers	592:623	The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out.					
31116962	2	74	dep	Phoenix	349:355	arg1	L.					369:370	Phoenix dactylifera L.	349:370	Phoenix dactylifera L.	349:370	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	7	75	theme	Morphological	1112:1124	arg1	analysis					1126:1133	Morphological analysis	1112:1133	Morphological analysis	1112:1133	Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites.					
31116962	2	76	theme	date	333:336	arg1	Phoenix					349:355	Phoenix	349:355	Phoenix	349:355	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	2	76	theme	date	333:336	arg1	fibers					373:378	different date palm tree (Phoenix dactylifera L.) fibers	323:378	different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method	323:434	In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method.					
31116962	1	77	theme	ecofriendly	224:234	arg1	materials					236:244	ecofriendly materials	224:244	ecofriendly materials	224:244	The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials.					
31888819	8	0	theme	Western	1645:1651	arg1	analysis					1658:1665	Western blot analysis	1645:1665	Western blot analysis	1645:1665	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	5	1	theme	Morphological	956:968	arg1	analyses					970:977	Morphological analyses	956:977	Morphological analyses	956:977	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	4	2	theme	molecular	752:760	arg1	weights					762:768	relative molecular weights	743:768	relative molecular weights of 12,724 Da	743:781	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	3	theme	BMP2/SMAD1	1932:1941	arg1	pathway					1953:1959	the BMP2/SMAD1 signaling pathway	1928:1959	the BMP2/SMAD1 signaling pathway	1928:1959	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	4	4	theme	monosaccharide	559:572	arg1	composition					574:584	monosaccharide composition	559:584	monosaccharide composition	559:584	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	2	5	theme	barometz	274:281	arg1	CB70					306:309	CB70	306:309	CB70	306:309	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	2	5	theme	barometz	274:281	arg1	polysaccharides					289:303	C. barometz crude polysaccharides	271:303	C. barometz crude polysaccharides (CB70)	271:310	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	4	6	dep	-D-Glcp-	726:733	arg1	→4					704:705	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	→4	704:705	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	6	dep	-D-Glcp-	726:733	arg1	→6					723:724	→6	723:724	→6	723:724	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	6	dep	-D-Glcp-	726:733	arg1	1→					716:717	1→	716:717	1→	716:717	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	6	dep	-D-Glcp-	726:733	arg1	D-Glcp-					674:680	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	D-Glcp-	674:680	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	6	dep	-D-Glcp-	726:733	arg1	→3,6					686:689	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	→3,6	686:689	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	6	7	theme	MC3T3-E1	1300:1307	arg1	cells					1309:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	1	8	theme	important	173:181	arg1	Cibotium					151:158	Cibotium barometz	151:167	Cibotium barometz	151:167	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	1	8	theme	important	173:181	arg1	medicine					203:210	an important traditional Chinese medicine	170:210	an important traditional Chinese medicine	170:210	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	5	9	from	irregular	1047:1055	arg1	shape					1069:1073	shape	1069:1073	shape	1069:1073	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	5	9	from	irregular	1047:1055	arg1	size					1060:1063	size	1060:1063	size	1060:1063	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	6	10	theme	mouse	1277:1281	arg1	cells					1309:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	1	11	theme	Chinese	195:201	arg1	Cibotium					151:158	Cibotium barometz	151:167	Cibotium barometz	151:167	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	1	11	theme	Chinese	195:201	arg1	medicine					203:210	an important traditional Chinese medicine	170:210	an important traditional Chinese medicine	170:210	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	7	12	theme	MC3T3-E1	1559:1566	arg1	cells					1568:1572	MC3T3-E1 cells	1559:1572	MC3T3-E1 cells	1559:1572	These results revealed that CBP70-1-1 and CBP70-1-2 significantly promoted the proliferation, differentiation and mineralization of MC3T3-E1 cells, even better than E2.					
31888819	5	13	from	shape	1069:1073	arg1	sheet					1031:1035	a sheet	1029:1035	a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth	1029:1162	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	5	13	from	shape	1069:1073	arg1	irregular					1047:1055	irregular	1047:1055	irregular	1047:1055	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	4	14	theme	analysis	608:615	arg1	combination					544:554	A combination	542:554	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis	542:615	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	0	15	theme	MC3T3-E1	135:142	arg1	cells					144:148	MC3T3-E1 cells	135:148	MC3T3-E1 cells	135:148	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	6	16	theme	activity	1367:1374	arg1	assay					1376:1380	alkaline phosphatase activity assay	1346:1380	alkaline phosphatase activity assay	1346:1380	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	5	17	from	size	1060:1063	arg1	sheet					1031:1035	a sheet	1029:1035	a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth	1029:1162	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	5	17	from	size	1060:1063	arg1	irregular					1047:1055	irregular	1047:1055	irregular	1047:1055	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	4	18	theme	molecular	926:934	arg1	weights					936:942	relative molecular weights	917:942	relative molecular weights of 3611 Da	917:953	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	19	theme	marker	1751:1756	arg1	genes					1758:1762	osteogenic-related marker genes	1732:1762	osteogenic-related marker genes (Runx2, Osx, Ocn and Opn)	1732:1788	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	19	theme	marker	1751:1756	arg1	Ocn					1777:1779	Ocn	1777:1779	Ocn	1777:1779	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	19	theme	marker	1751:1756	arg1	Osx					1772:1774	Osx	1772:1774	Osx	1772:1774	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	19	theme	marker	1751:1756	arg1	Opn					1785:1787	Opn	1785:1787	Opn	1785:1787	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	19	theme	marker	1751:1756	arg1	Runx2					1765:1769	Runx2	1765:1769	Runx2	1765:1769	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	6	20	theme	alkaline	1346:1353	arg1	phosphatase					1355:1365	alkaline phosphatase	1346:1365	alkaline phosphatase activity assay	1346:1380	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	21	dep	-L-Araf-	900:907	arg1	→3					897:898	→3	897:898	→3	897:898	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	21	dep	-L-Araf-	900:907	arg1	→4,6					876:879	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	→4,6	876:879	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	21	dep	-L-Araf-	900:907	arg1	→3,6					842:845	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	→3,6	842:845	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	21	dep	-L-Araf-	900:907	arg1	1→					890:891	1→	890:891	1→	890:891	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	21	dep	-L-Araf-	900:907	arg1	D-Glcp-					830:836	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	D-Glcp-	830:836	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	22	theme	1→	826:827	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	23	dep	proteins	1794:1801	arg1	BMP2					1804:1807	BMP2	1804:1807	BMP2	1804:1807	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	23	dep	proteins	1794:1801	arg1	p-SMAD1					1825:1831	p-SMAD1	1825:1831	p-SMAD1	1825:1831	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	23	dep	proteins	1794:1801	arg1	OSX					1817:1819	OSX	1817:1819	OSX	1817:1819	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	23	dep	proteins	1794:1801	arg1	proteins					1794:1801	proteins	1794:1801	proteins (BMP2, RUNX2, OSX and p-SMAD1)	1794:1832	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	23	dep	proteins	1794:1801	arg1	RUNX2					1810:1814	RUNX2	1810:1814	RUNX2	1810:1814	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	9	24	theme	natural	2010:2016	arg1	CBP70-1-2					1985:1993	CBP70-1-2	1985:1993	CBP70-1-2	1985:1993	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	9	24	theme	natural	2010:2016	arg1	agent					2036:2040	a potential natural anti-osteoporotic agent	1998:2040	a potential natural anti-osteoporotic agent for pharmacotherapy	1998:2060	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	4	25	theme	-D-Glcp-	817:824	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	2	26	theme	OVX	391:393	arg1	rats					395:398	OVX rats	391:398	OVX rats	391:398	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	0	27	theme	BMP2/SMAD1	103:112	arg1	pathway					124:130	the BMP2/SMAD1 signaling pathway	99:130	the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells	99:148	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	6	28	theme	alizarin	1387:1394	arg1	assay					1406:1410	alizarin red-based assay	1387:1410	alizarin red-based assay	1387:1410	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	29	dep	composed	646:653	arg1	1→					735:736	1→	735:736	1→	735:736	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	30	theme	-D-Manp-	847:854	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	0	31	from	rhizomes	31:38	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from rhizomes of Cibotium barometz	0:59	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	8	32	theme	proteins	1794:1801	arg1	expression					1718:1727	the expression	1714:1727	the expression	1714:1727	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	3	33	dep	polysaccharides	475:489	arg1	polysaccharides					475:489	two homogeneous polysaccharides	459:489	two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2)	459:515	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	33	dep	polysaccharides	475:489	arg1	CBP70-1-2					506:514	CBP70-1-2	506:514	CBP70-1-2	506:514	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	33	dep	polysaccharides	475:489	arg1	CBP70-1-1					492:500	CBP70-1-1	492:500	CBP70-1-1	492:500	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	8	34	dep	genes	1758:1762	arg1	Opn					1785:1787	Opn	1785:1787	Opn	1785:1787	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	34	dep	genes	1758:1762	arg1	Ocn					1777:1779	Ocn	1777:1779	Ocn	1777:1779	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	34	dep	genes	1758:1762	arg1	genes					1758:1762	osteogenic-related marker genes	1732:1762	osteogenic-related marker genes (Runx2, Osx, Ocn and Opn)	1732:1788	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	34	dep	genes	1758:1762	arg1	Osx					1772:1774	Osx	1772:1774	Osx	1772:1774	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	34	dep	genes	1758:1762	arg1	Runx2					1765:1769	Runx2	1765:1769	Runx2	1765:1769	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	4	35	theme	1→	838:839	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	5	36	theme	protuberances	1125:1137	arg1	full					1111:1114	full	1111:1114	full	1111:1114	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	5	36	theme	protuberances	1125:1137	arg1	surface					1086:1092	the surface	1082:1092	the surface of CBP70-1-1	1082:1105	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	0	37	theme	Cibotium	43:50	arg1	barometz					52:59	Cibotium barometz	43:59	Cibotium barometz	43:59	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	4	38	theme	1→	872:873	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	39	theme	CBP70-1-2	1881:1889	arg1	activity					1869:1876	the osteogenic activity	1854:1876	the osteogenic activity of CBP70-1-2	1854:1889	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	4	40	theme	composition	574:584	arg1	combination					544:554	A combination	542:554	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis	542:615	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	41	theme	FT-IR	587:591	arg1	combination					544:554	A combination	542:554	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis	542:615	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	42	theme	blot	1653:1656	arg1	analysis					1658:1665	Western blot analysis	1645:1665	Western blot analysis	1645:1665	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	3	43	theme	homogeneous	463:473	arg1	polysaccharides					475:489	two homogeneous polysaccharides	459:489	two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2)	459:515	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	43	theme	homogeneous	463:473	arg1	CBP70-1-2					506:514	CBP70-1-2	506:514	CBP70-1-2	506:514	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	43	theme	homogeneous	463:473	arg1	CBP70-1-1					492:500	CBP70-1-1	492:500	CBP70-1-1	492:500	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	6	44	theme	CBP70-1-2	1207:1215	arg1	effects					1182:1188	the effects	1178:1188	the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells	1178:1313	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	45	theme	-D-Galp-	863:870	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	2	46	theme	crude	283:287	arg1	CB70					306:309	CB70	306:309	CB70	306:309	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	2	46	theme	crude	283:287	arg1	polysaccharides					289:303	C. barometz crude polysaccharides	271:303	C. barometz crude polysaccharides (CB70)	271:310	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	6	47	theme	CBP70-1-1	1193:1201	arg1	effects					1182:1188	the effects	1178:1188	the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells	1178:1313	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	48	theme	relative	743:750	arg1	weights					762:768	relative molecular weights	743:768	relative molecular weights of 12,724 Da	743:781	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	6	49	theme	cells	1309:1313	arg1	proliferation					1224:1236	proliferation	1224:1236	proliferation	1224:1236	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	6	49	theme	cells	1309:1313	arg1	mineralization					1259:1272	mineralization	1259:1272	mineralization	1259:1272	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	6	49	theme	cells	1309:1313	arg1	differentiation					1239:1253	differentiation	1239:1253	differentiation	1239:1253	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	7	50	theme	cells	1568:1572	arg1	differentiation					1521:1535	differentiation	1521:1535	differentiation	1521:1535	These results revealed that CBP70-1-1 and CBP70-1-2 significantly promoted the proliferation, differentiation and mineralization of MC3T3-E1 cells, even better than E2.					
31888819	7	50	theme	cells	1568:1572	arg1	proliferation					1506:1518	proliferation	1506:1518	proliferation	1506:1518	These results revealed that CBP70-1-1 and CBP70-1-2 significantly promoted the proliferation, differentiation and mineralization of MC3T3-E1 cells, even better than E2.					
31888819	7	50	theme	cells	1568:1572	arg1	mineralization					1541:1554	mineralization	1541:1554	mineralization	1541:1554	These results revealed that CBP70-1-1 and CBP70-1-2 significantly promoted the proliferation, differentiation and mineralization of MC3T3-E1 cells, even better than E2.					
31888819	8	51	theme	signaling	1943:1951	arg1	pathway					1953:1959	the BMP2/SMAD1 signaling pathway	1928:1959	the BMP2/SMAD1 signaling pathway	1928:1959	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	6	52	theme	pre-osteoblastic	1283:1298	arg1	cells					1309:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	mouse pre-osteoblastic MC3T3-E1 cells	1277:1313	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	1	53	theme	traditional	183:193	arg1	Cibotium					151:158	Cibotium barometz	151:167	Cibotium barometz	151:167	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	1	53	theme	traditional	183:193	arg1	medicine					203:210	an important traditional Chinese medicine	170:210	an important traditional Chinese medicine	170:210	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	4	54	theme	-D-Glcp-	881:888	arg1	-L-Araf-					900:907	→4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→	814:910	-L-Araf-	900:907	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	55	theme	GC-MS	594:598	arg1	combination					544:554	A combination	542:554	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis	542:615	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	56	theme	osteogenic-related	1732:1749	arg1	genes					1758:1762	osteogenic-related marker genes	1732:1762	osteogenic-related marker genes (Runx2, Osx, Ocn and Opn)	1732:1788	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	56	theme	osteogenic-related	1732:1749	arg1	Ocn					1777:1779	Ocn	1777:1779	Ocn	1777:1779	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	56	theme	osteogenic-related	1732:1749	arg1	Osx					1772:1774	Osx	1772:1774	Osx	1772:1774	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	56	theme	osteogenic-related	1732:1749	arg1	Opn					1785:1787	Opn	1785:1787	Opn	1785:1787	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	8	56	theme	osteogenic-related	1732:1749	arg1	Runx2					1765:1769	Runx2	1765:1769	Runx2	1765:1769	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	4	57	theme	NMR	604:606	arg1	analysis					608:615	NMR analysis	604:615	NMR analysis	604:615	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	58	theme	genes	1758:1762	arg1	expression					1718:1727	the expression	1714:1727	the expression	1714:1727	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	6	59	from	effects	1182:1188	arg1	proliferation					1224:1236	proliferation	1224:1236	proliferation	1224:1236	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	6	59	from	effects	1182:1188	arg1	mineralization					1259:1272	mineralization	1259:1272	mineralization	1259:1272	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	6	59	from	effects	1182:1188	arg1	differentiation					1239:1253	differentiation	1239:1253	differentiation	1239:1253	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	6	60	theme	phosphatase	1355:1365	arg1	assay					1376:1380	alkaline phosphatase activity assay	1346:1380	alkaline phosphatase activity assay	1346:1380	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	61	theme	3611 Da	947:953	arg1	weights					936:942	relative molecular weights	917:942	relative molecular weights of 3611 Da	917:953	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	62	theme	-D-Galp-	661:668	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	63	theme	12,724 Da	773:781	arg1	weights					762:768	relative molecular weights	743:768	relative molecular weights of 12,724 Da	743:781	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	4	64	theme	1→	670:671	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	0	65	theme	bone	69:72	arg1	formation					74:82	bone formation	69:82	bone formation	69:82	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	6	66	theme	CCK-8	1333:1337	arg1	assay					1339:1343	CCK-8 assay	1333:1343	CCK-8 assay	1333:1343	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	67	theme	relative	917:924	arg1	weights					936:942	relative molecular weights	917:942	relative molecular weights of 3611 Da	917:953	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	9	68	theme	potential	2000:2008	arg1	CBP70-1-2					1985:1993	CBP70-1-2	1985:1993	CBP70-1-2	1985:1993	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	9	68	theme	potential	2000:2008	arg1	agent					2036:2040	a potential natural anti-osteoporotic agent	1998:2040	a potential natural anti-osteoporotic agent for pharmacotherapy	1998:2060	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	9	69	theme	anti-osteoporotic	2018:2034	arg1	CBP70-1-2					1985:1993	CBP70-1-2	1985:1993	CBP70-1-2	1985:1993	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	9	69	theme	anti-osteoporotic	2018:2034	arg1	agent					2036:2040	a potential natural anti-osteoporotic agent	1998:2040	a potential natural anti-osteoporotic agent for pharmacotherapy	1998:2060	These findings suggest CBP70-1-2 as a potential natural anti-osteoporotic agent for pharmacotherapy.					
31888819	0	70	theme	signaling	114:122	arg1	pathway					124:130	the BMP2/SMAD1 signaling pathway	99:130	the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells	99:148	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	6	71	theme	red-based	1396:1404	arg1	assay					1406:1410	alizarin red-based assay	1387:1410	alizarin red-based assay	1387:1410	Furthermore, the effects of CBP70-1-1 and CBP70-1-2 on the proliferation, differentiation and mineralization of mouse pre-osteoblastic MC3T3-E1 cells were assessed via CCK-8 assay, alkaline phosphatase activity assay, and alizarin red-based assay, respectively.					
31888819	4	72	theme	-D-Manp-	691:698	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	3	73	theme	active	429:434	arg1	s					447:447	s	447:447	s	447:447	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	73	theme	active	429:434	arg1	ingredient					436:445	biological active ingredient	418:445	biological active ingredient(s) of CB70	418:456	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	0	74	theme	novel	4:8	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from rhizomes of Cibotium barometz	0:59	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	8	75	theme	quantitative	1614:1625	arg1	PCR					1637:1639	quantitative real-time PCR	1614:1639	quantitative real-time PCR	1614:1639	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	2	76	theme	biomechanical	363:375	arg1	properties					377:386	the biomechanical properties	359:386	the biomechanical properties of OVX rats	359:398	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	4	77	with	-L-Araf-	900:907	arg1	weights					936:942	relative molecular weights	917:942	relative molecular weights of 3611 Da	917:953	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	1	78	used	used	216:219	arg2	Cibotium					151:158	Cibotium barometz	151:167	Cibotium barometz	151:167	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	1	78	used	used	216:219	arg2	medicine					203:210	an important traditional Chinese medicine	170:210	an important traditional Chinese medicine	170:210	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	4	79	theme	1→	682:683	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	8	80	theme	osteogenic	1858:1867	arg1	activity					1869:1876	the osteogenic activity	1854:1876	the osteogenic activity of CBP70-1-2	1854:1889	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	3	81	theme	biological	418:427	arg1	s					447:447	s	447:447	s	447:447	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	81	theme	biological	418:427	arg1	ingredient					436:445	biological active ingredient	418:445	biological active ingredient(s) of CB70	418:456	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	4	82	theme	-D-Glcp-	707:714	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	5	83	theme	sharp	1119:1123	arg1	protuberances					1125:1137	sharp protuberances	1119:1137	sharp protuberances	1119:1137	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	2	84	theme	rats	395:398	arg1	properties					377:386	the biomechanical properties	359:386	the biomechanical properties of OVX rats	359:398	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	0	85	theme	barometz	52:59	arg1	rhizomes					31:38	rhizomes	31:38	rhizomes of Cibotium barometz	31:59	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31888819	2	86	theme	bone	328:331	arg1	loss					333:336	bone loss	328:336	bone loss	328:336	We found that C. barometz crude polysaccharides (CB70) could alleviate bone loss and markedly improve the biomechanical properties of OVX rats.					
31888819	8	87	theme	real-time	1627:1635	arg1	PCR					1637:1639	quantitative real-time PCR	1614:1639	quantitative real-time PCR	1614:1639	More importantly, quantitative real-time PCR and Western blot analysis indicated that CBP70-1-2 pronouncedly promoted the expression of osteogenic-related marker genes (Runx2, Osx, Ocn and Opn) and proteins (BMP2, RUNX2, OSX and p-SMAD1), which implies that the osteogenic activity of CBP70-1-2 is accomplished mainly by activating the BMP2/SMAD1 signaling pathway.					
31888819	4	88	theme	1→	700:701	arg1	-D-Glcp-					726:733	→6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-	658:733	-D-Glcp-	726:733	A combination of monosaccharide composition, FT-IR, GC-MS and NMR analysis indicated that CBP70-1-1 was composed of →6)-D-Galp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →4)-D-Glcp-(1→ and →6)-D-Glcp-(1→ with relative molecular weights of 12,724 Da, and CBP70-1-2 was composed of →4)-D-Glcp-(1→, D-Glcp-(1→, →3,6)-D-Manp-(1→, →6)-D-Galp-(1→, →4,6)-D-Glcp-(1→ and →3)-L-Araf-(1→ with relative molecular weights of 3611 Da.					
31888819	5	89	theme	CBP70-1-1	1097:1105	arg1	full					1111:1114	full	1111:1114	full	1111:1114	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	5	89	theme	CBP70-1-1	1097:1105	arg1	surface					1086:1092	the surface	1082:1092	the surface of CBP70-1-1	1082:1105	Morphological analyses revealed that CBP70-1-1 and CBP70-1-2 appeared as a sheet that were irregular in size and shape, while the surface of CBP70-1-1 was full of sharp protuberances and CBP70-1-2 was smooth.					
31888819	3	90	theme	CB70	453:456	arg1	s					447:447	s	447:447	s	447:447	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	3	90	theme	CB70	453:456	arg1	ingredient					436:445	biological active ingredient	418:445	biological active ingredient(s) of CB70	418:456	Thus, to clarify biological active ingredient(s) of CB70, two homogeneous polysaccharides (CBP70-1-1 and CBP70-1-2) were purified from CB70.					
31888819	1	91	theme	strengthening	224:236	arg1	bones					238:242	bones	238:242	bones	238:242	Cibotium barometz, an important traditional Chinese medicine, is used in strengthening bones and tendons.					
31888819	0	92	from	pathway	124:130	arg1	cells					144:148	MC3T3-E1 cells	135:148	MC3T3-E1 cells	135:148	Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2/SMAD1 signaling pathway in MC3T3-E1 cells.					
31024890	15	0	theme	similarities	2409:2420	arg1	understanding					2392:2404	The molecular understanding	2378:2404	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps	2378:2484	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	2	1	theme	other	172:176	arg1	derivatives					191:201	other carbohydrate derivatives	172:201	other carbohydrate derivatives	172:201	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	9	2	theme	atom	1354:1357	arg1	charge					1333:1338	the more negative charge	1315:1338	the more negative charge of the oxygen atom of water	1315:1366	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	2	3	theme	oligosaccharides	151:166	arg1	synthesis					138:146	The synthesis	134:146	The synthesis of oligosaccharides and other carbohydrate derivatives	134:201	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	9	4	theme	water	1362:1366	arg1	atom					1354:1357	the oxygen atom	1343:1357	the oxygen atom of water	1343:1366	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	14	5	theme	Glu392-Fuc	2208:2217	arg1	interaction					2219:2229	the Glu392-Fuc interaction	2204:2229	the Glu392-Fuc interaction	2204:2229	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	12	6	theme	free	1824:1827	arg1	maxima					1836:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	7	7	theme	glycosyl	997:1004	arg1	hydrolase					1006:1014	a retaining glycosyl hydrolase	985:1014	a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally	985:1093	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	7	theme	glycosyl	997:1004	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	11	8	dep	O4GLC-C1FUC	1620:1630	arg1	forming					1633:1639	forming	1633:1639	forming bond	1633:1644	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	8	9	theme	oxocarbenium	1138:1149	arg1	character					1151:1159	a strong oxocarbenium character	1129:1159	a strong oxocarbenium character	1129:1159	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	13	10	theme	Glc	1979:1981	arg1	group					1957:1961	The 3-OHGLC group	1945:1961	The 3-OHGLC group of the acceptor Glc (transglycosylation)	1945:2002	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	10	theme	Glc	1979:1981	arg1	transglycosylation					1984:2001	transglycosylation	1984:2001	transglycosylation	1984:2001	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	10	theme	Glc	1979:1981	arg1	WAT432					2008:2013	WAT432	2008:2013	WAT432 (neighbor water in hydrolysis)	2008:2044	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	10	theme	Glc	1979:1981	arg1	Glc					1979:1981	the acceptor Glc	1966:1981	the acceptor Glc	1966:1981	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	3	11	theme	glycosyl	315:322	arg1	GHs					336:338	GHs	336:338	GHs	336:338	For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of glycosidic bonds.					
31024890	3	11	theme	glycosyl	315:322	arg1	hydrolases					324:333	glycosyl hydrolases	315:333	glycosyl hydrolases (GHs)	315:339	For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of glycosidic bonds.					
31024890	4	12	theme	transglycosylation	451:468	arg1	reaction					474:481	a transglycosylation (T) reaction	449:481	a transglycosylation (T) reaction that competes with hydrolysis (H)	449:515	In particular, retaining GHs can catalyze a transglycosylation (T) reaction that competes with hydrolysis (H).					
31024890	12	13	theme	structural	1920:1929	arg1	fluctuations					1931:1942	significant structural fluctuations	1908:1942	significant structural fluctuations	1908:1942	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	13	14	dep	WAT432	2008:2013	arg1	water					2025:2029	neighbor water	2016:2029	neighbor water in hydrolysis	2016:2043	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	7	15	theme	type	931:934	arg1	GH1					979:981	family GH1	972:981	family GH1	972:981	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	15	theme	type	931:934	arg1	hydrolase					1006:1014	a retaining glycosyl hydrolase	985:1014	a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally	985:1093	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	15	theme	type	931:934	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	2	16	theme	applied	284:290	arg1	level					292:296	the fundamental and applied level	264:296	the fundamental and applied level	264:296	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	14	17	theme	T/H	2250:2252	arg1	ratio					2254:2258	the T/H ratio	2246:2258	the T/H ratio	2246:2258	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	7	18	theme	family	972:977	arg1	GH1					979:981	family GH1	972:981	family GH1	972:981	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	18	theme	family	972:977	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	19	theme	Thermus	936:942	arg1	GH1					979:981	family GH1	972:981	family GH1	972:981	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	19	theme	Thermus	936:942	arg1	hydrolase					1006:1014	a retaining glycosyl hydrolase	985:1014	a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally	985:1093	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	19	theme	Thermus	936:942	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	12	20	theme	hydrolysis	1885:1894	arg1	TS					1896:1897	the hydrolysis TS	1881:1897	the hydrolysis TS	1881:1897	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	14	21	theme	interaction	2167:2177	arg1	analysis					2155:2162	The analysis	2151:2162	The analysis of interaction	2151:2177	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	11	22	theme	O4GLC-C1FUC	1620:1630	arg1	distance					1647:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	9	23	theme	atomic	1208:1213	arg1	charges					1215:1221	The atomic charges	1204:1221	The atomic charges at the transition states for hydrolysis and transglycosylation	1204:1284	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	9	23	theme	atomic	1208:1213	arg1	similar					1295:1301	similar	1295:1301	similar	1295:1301	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	11	24	from	base	1777:1780	arg1	TS					1800:1801	the hydrolysis TS	1785:1801	the hydrolysis TS	1785:1801	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	13	25	with	interaction	2117:2127	arg1	O5FUC					2134:2138	O5FUC	2134:2138	O5FUC	2134:2138	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	25	with	interaction	2117:2127	arg1	O4FUC					2144:2148	O4FUC	2144:2148	O4FUC	2144:2148	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	5	26	theme	wild	579:582	arg1	GHs					589:591	wild type GHs	579:591	wild type GHs	579:591	This has been done either employing controlled conditions in wild type GHs or by engineering new mutants.					
31024890	10	27	contain	has	1446:1448	arg1	state					1440:1444	The glycosylation transition state	1411:1444	The glycosylation transition state	1411:1444	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	10	27	contain	has	1446:1448	arg2	character					1465:1473	a stronger S 2 character	1450:1473	a stronger S 2 character than the deglycosylation ones	1450:1503	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	7	28	dep	glycosylation	858:870	arg1	the					854:856	the	854:856	the	854:856	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	15	29	theme	glycan	2539:2544	arg1	synthesis					2546:2554	glycan synthesis	2539:2554	glycan synthesis	2539:2554	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	9	30	theme	transition	1230:1239	arg1	states					1241:1246	the transition states	1226:1246	the transition states for hydrolysis and transglycosylation	1226:1284	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	0	31	theme	Thermus	67:73	arg1	β-Glycosidase					88:100	Thermus thermophilus β-Glycosidase	67:100	Thermus thermophilus β-Glycosidase	67:100	Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase.					
31024890	4	32	theme	T	471:471	arg1	reaction					474:481	a transglycosylation (T) reaction	449:481	a transglycosylation (T) reaction that competes with hydrolysis (H)	449:515	In particular, retaining GHs can catalyze a transglycosylation (T) reaction that competes with hydrolysis (H).					
31024890	7	33	dep	Thermus	936:942	arg1	thermophilus					944:955	thermophilus	944:955	thermophilus	944:955	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	11	34	theme	Glu164	1770:1775	arg1	base					1777:1780	the Glu164 base	1766:1780	the Glu164 base in the hydrolysis TS	1766:1801	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	15	35	theme	biocatalysts	2522:2533	arg1	design					2508:2513	the design	2504:2513	the design of new biocatalysts for glycan synthesis	2504:2554	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	14	36	dep	increase	2237:2244	arg1	either					2261:2266	either	2261:2266	either	2261:2266	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	14	36	dep	increase	2237:2244	arg1	indirectly					2306:2315	indirectly	2306:2315	indirectly	2306:2315	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	11	37	theme	hydrolysis	1789:1798	arg1	TS					1800:1801	the hydrolysis TS	1785:1801	the hydrolysis TS	1785:1801	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	1	38	theme	QM/MM	121:125	arg1	Study					127:131	QM/MM Study	121:131	QM/MM Study	121:131	A Combined MD and QM/MM Study.					
31024890	3	39	theme	bonds	400:404	arg1	synthesis					376:384	the synthesis	372:384	the synthesis of glycosidic bonds	372:404	For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of glycosidic bonds.					
31024890	13	40	from	water	2025:2029	arg1	hydrolysis					2034:2043	hydrolysis	2034:2043	hydrolysis	2034:2043	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	2	41	theme	carbohydrate	178:189	arg1	derivatives					191:201	other carbohydrate derivatives	172:201	other carbohydrate derivatives	172:201	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	10	42	theme	transition	1429:1438	arg1	state					1440:1444	The glycosylation transition state	1411:1444	The glycosylation transition state	1411:1444	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	11	43	theme	shorter	1612:1618	arg1	distance					1647:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	6	44	theme	moderate	693:700	arg1	success					702:708	moderate success	693:708	moderate success in several cases	693:725	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	6	45	theme	T/H	773:775	arg1	modulation					777:786	T/H modulation	773:786	T/H modulation	773:786	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	14	46	theme	residue	2295:2301	arg1	mutation					2278:2285	direct mutation	2271:2285	direct mutation of this residue	2271:2301	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	7	47	used	used	814:817	arg2	we					806:807	we	806:807	we	806:807	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	9	48	theme	negative	1324:1331	arg1	charge					1333:1338	the more negative charge	1315:1338	the more negative charge of the oxygen atom of water	1315:1366	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	12	49	theme	inverted	1852:1859	arg1	situation					1861:1869	the inverted situation	1848:1869	the inverted situation	1848:1869	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	10	50	theme	stronger	1452:1459	arg1	character					1465:1473	a stronger S 2 character	1450:1473	a stronger S 2 character than the deglycosylation ones	1450:1503	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	11	51	theme	QM	1554:1555	arg1	level					1571:1575	the QM(PBE0/TZVP)/MM level	1550:1575	the QM(PBE0/TZVP)/MM level	1550:1575	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	52	theme	HACC	1741:1744	arg1	proton					1746:1751	the HACC proton	1737:1751	the HACC proton	1737:1751	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	52	theme	HACC	1741:1744	arg1	closer					1756:1761	closer	1756:1761	closer	1756:1761	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	10	53	theme	deglycosylation	1484:1498	arg1	ones					1500:1503	the deglycosylation ones	1480:1503	the deglycosylation ones	1480:1503	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	7	54	theme	transglycosylation	1028:1045	arg1	yield					1047:1051	a transglycosylation yield	1026:1051	a transglycosylation yield of 36%	1026:1058	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	6	55	theme	molecular	753:761	arg1	unclear					792:798	unclear	792:798	unclear	792:798	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	6	55	theme	molecular	753:761	arg1	basis					763:767	the molecular basis	749:767	the molecular basis for T/H modulation	749:786	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	9	56	theme	oxygen	1347:1352	arg1	atom					1354:1357	the oxygen atom	1343:1357	the oxygen atom of water	1343:1366	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	12	57	theme	energy	1829:1834	arg1	maxima					1836:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	8	58	theme	transition	1106:1115	arg1	states					1117:1122	The three transition states	1096:1122	The three transition states	1096:1122	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	8	59	theme	ring	1165:1168	arg1	conformations					1170:1182	ring conformations	1165:1182	ring conformations	1165:1182	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	10	60	theme	proton	1513:1518	arg1	transfer					1520:1527	the proton transfer	1509:1527	the proton transfer	1509:1527	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	10	60	theme	proton	1513:1518	arg1	advanced					1537:1544	advanced	1537:1544	advanced	1537:1544	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	11	61	theme	PBE0/TZVP	1557:1565	arg1	level					1571:1575	the QM(PBE0/TZVP)/MM level	1550:1575	the QM(PBE0/TZVP)/MM level	1550:1575	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	5	62	theme	new	611:613	arg1	mutants					615:621	new mutants	611:621	new mutants	611:621	This has been done either employing controlled conditions in wild type GHs or by engineering new mutants.					
31024890	12	63	theme	SCC-DFTB	1811:1818	arg1	maxima					1836:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	7	64	theme	retaining	987:995	arg1	hydrolase					1006:1014	a retaining glycosyl hydrolase	985:1014	a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally	985:1093	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	64	theme	retaining	987:995	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	9	65	from	states	1241:1246	arg1	charges					1215:1221	The atomic charges	1204:1221	The atomic charges at the transition states for hydrolysis and transglycosylation	1204:1284	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	9	65	from	states	1241:1246	arg1	similar					1295:1301	similar	1295:1301	similar	1295:1301	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	3	66	theme	many	303:306	arg1	years					308:312	many years	303:312	many years	303:312	For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of glycosidic bonds.					
31024890	13	67	theme	acceptor	1970:1977	arg1	Glc					1979:1981	the acceptor Glc	1966:1981	the acceptor Glc	1966:1981	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	8	68	theme	strong	1131:1136	arg1	character					1151:1159	a strong oxocarbenium character	1129:1159	a strong oxocarbenium character	1129:1159	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	14	69	dep	either	2261:2266	arg1	mutation					2278:2285	direct mutation	2271:2285	direct mutation of this residue	2271:2301	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	2	70	theme	glycosciences	242:254	arg1	advancement					227:237	the advancement	223:237	the advancement of glycosciences	223:254	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	9	71	theme	acceptor	1397:1404	arg1	Glc					1406:1408	the acceptor Glc	1393:1408	the acceptor Glc	1393:1408	The atomic charges at the transition states for hydrolysis and transglycosylation are very similar, except for the more negative charge of the oxygen atom of water when compared to that of the acceptor Glc.					
31024890	12	72	theme	QM	1808:1809	arg1	maxima					1836:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima	1804:1841	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	4	73	theme	retaining	422:430	arg1	GHs					432:434	retaining GHs	422:434	retaining GHs	422:434	In particular, retaining GHs can catalyze a transglycosylation (T) reaction that competes with hydrolysis (H).					
31024890	12	74	theme	significant	1908:1918	arg1	fluctuations					1931:1942	significant structural fluctuations	1908:1942	significant structural fluctuations	1908:1942	The QM(SCC-DFTB)/MM free energy maxima show the inverted situation, although the hydrolysis TS presents significant structural fluctuations.					
31024890	13	75	theme	3-OHGLC	1949:1955	arg1	group					1957:1961	The 3-OHGLC group	1945:1961	The 3-OHGLC group of the acceptor Glc (transglycosylation)	1945:2002	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	75	theme	3-OHGLC	1949:1955	arg1	transglycosylation					1984:2001	transglycosylation	1984:2001	transglycosylation	1984:2001	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	13	75	theme	3-OHGLC	1949:1955	arg1	Glc					1979:1981	the acceptor Glc	1966:1981	the acceptor Glc	1966:1981	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	8	76	contain	have	1124:1127	arg2	character					1151:1159	a strong oxocarbenium character	1129:1159	a strong oxocarbenium character	1129:1159	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	8	76	contain	have	1124:1127	arg1	states					1117:1122	The three transition states	1096:1122	The three transition states	1096:1122	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	8	76	contain	have	1124:1127	arg2	conformations					1170:1182	ring conformations	1165:1182	ring conformations	1165:1182	The three transition states have a strong oxocarbenium character and ring conformations between 4H3 and 4E.					
31024890	5	77	theme	type	584:587	arg1	GHs					589:591	wild type GHs	579:591	wild type GHs	579:591	This has been done either employing controlled conditions in wild type GHs or by engineering new mutants.					
31024890	11	78	dep	longer	1660:1665	arg1	breaking					1684:1691	breaking	1684:1691	breaking	1684:1691	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	78	dep	longer	1660:1665	arg1	OE2GLU338-C1FUC					1667:1681	OE2GLU338-C1FUC	1667:1681	OE2GLU338-C1FUC (breaking)	1667:1692	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	2	79	theme	fundamental	268:278	arg1	level					292:296	the fundamental and applied level	264:296	the fundamental and applied level	264:296	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	7	80	theme	wild	926:929	arg1	GH1					979:981	family GH1	972:981	family GH1	972:981	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	80	theme	wild	926:929	arg1	hydrolase					1006:1014	a retaining glycosyl hydrolase	985:1014	a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally	985:1093	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	7	80	theme	wild	926:929	arg1	β-glycosidase					957:969	wild type Thermus thermophilus β-glycosidase	926:969	wild type Thermus thermophilus β-glycosidase (family GH1)	926:982	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	13	81	theme	transition	2091:2100	arg1	states					2102:2107	the oxocarbenium transition states	2074:2107	the oxocarbenium transition states	2074:2107	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	11	82	contain	has	1608:1610	arg2	distance					1647:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	shorter O4GLC-C1FUC (forming bond) distance	1612:1654	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	82	contain	has	1608:1610	arg1	TS					1582:1583	the TS	1578:1583	the TS for transglycosylation	1578:1606	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	82	contain	has	1608:1610	arg2	distance					1694:1701	longer OE2GLU338-C1FUC (breaking) distance	1660:1701	longer OE2GLU338-C1FUC (breaking) distance	1660:1701	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	11	83	theme	longer	1660:1665	arg1	distance					1694:1701	longer OE2GLU338-C1FUC (breaking) distance	1660:1701	longer OE2GLU338-C1FUC (breaking) distance	1660:1701	At the QM(PBE0/TZVP)/MM level, the TS for transglycosylation has shorter O4GLC-C1FUC (forming bond) distance and longer OE2GLU338-C1FUC (breaking) distance than the hydrolysis one, although the HACC proton is closer to the Glu164 base in the hydrolysis TS.					
31024890	7	84	theme	transglycosylation	888:905	arg1	steps					907:911	transglycosylation steps	888:911	transglycosylation steps	888:911	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	15	85	theme	new	2518:2520	arg1	biocatalysts					2522:2533	new biocatalysts	2518:2533	new biocatalysts for glycan synthesis	2518:2554	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	15	86	theme	molecular	2382:2390	arg1	understanding					2392:2404	The molecular understanding	2378:2404	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps	2378:2484	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	14	87	dep	Asn390I	2351:2357	arg1	cases					2371:2375	cases	2371:2375	cases	2371:2375	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	14	87	dep	Asn390I	2351:2357	arg1	the					2347:2349	the	2347:2349	the	2347:2349	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	6	88	theme	T/H	659:661	arg1	ratio					663:667	the T/H ratio	655:667	the T/H ratio	655:667	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	0	89	theme	Hydrolysis	10:19	arg1	Reactions					44:52	Hydrolysis and Transglycosylation Reactions	10:52	Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase	10:100	Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase.					
31024890	5	90	theme	controlled	554:563	arg1	conditions					565:574	controlled conditions	554:574	controlled conditions	554:574	This has been done either employing controlled conditions in wild type GHs or by engineering new mutants.					
31024890	1	91	theme	Combined	105:112	arg1	MD					114:115	A Combined MD	103:115	A Combined MD	103:115	A Combined MD and QM/MM Study.					
31024890	3	92	theme	glycosidic	389:398	arg1	bonds					400:404	glycosidic bonds	389:404	glycosidic bonds	389:404	For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of glycosidic bonds.					
31024890	0	93	theme	Transglycosylation	25:42	arg1	Reactions					44:52	Hydrolysis and Transglycosylation Reactions	10:52	Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase	10:100	Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase.					
31024890	13	94	theme	oxocarbenium	2078:2089	arg1	states					2102:2107	the oxocarbenium transition states	2074:2107	the oxocarbenium transition states	2074:2107	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	0	95	dep	Thermus	67:73	arg1	thermophilus					75:86	thermophilus	75:86	thermophilus	75:86	Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase.					
31024890	2	96	theme	derivatives	191:201	arg1	synthesis					138:146	The synthesis	134:146	The synthesis of oligosaccharides and other carbohydrate derivatives	134:201	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	6	97	theme	several	713:719	arg1	cases					721:725	several cases	713:725	several cases	713:725	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	7	98	theme	%	1058:1058	arg1	yield					1047:1051	a transglycosylation yield	1026:1051	a transglycosylation yield of 36%	1026:1058	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	6	99	from	success	702:708	arg1	cases					721:725	several cases	713:725	several cases	713:725	The goal, which is to increase the T/H ratio, has been achieved with moderate success in several cases despite the fact that the molecular basis for T/H modulation are unclear.					
31024890	15	100	theme	transglycosylation	2461:2478	arg1	steps					2480:2484	transglycosylation steps	2461:2484	transglycosylation steps	2461:2484	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	10	101	theme	glycosylation	1415:1427	arg1	state					1440:1444	The glycosylation transition state	1411:1444	The glycosylation transition state	1411:1444	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	14	102	theme	direct	2271:2276	arg1	mutation					2278:2285	direct mutation	2271:2285	direct mutation of this residue	2271:2301	The analysis of interaction suggests that perturbing the Glu392-Fuc interaction could increase the T/H ratio, either by direct mutation of this residue or indirectly as reported experimentally in the Asn390I and Phe401S cases.					
31024890	7	103	theme	DFT	822:824	arg1	calculations					830:841	QM(DFT)/MM calculations	819:841	QM(DFT)/MM calculations	819:841	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31024890	13	104	theme	neighbor	2016:2023	arg1	water					2025:2029	neighbor water	2016:2029	neighbor water in hydrolysis	2016:2043	The 3-OHGLC group of the acceptor Glc (transglycosylation) and WAT432 (neighbor water in hydrolysis) are identified to stabilize the oxocarbenium transition states through interaction with O5FUC and O4FUC.					
31024890	15	105	theme	differences	2426:2436	arg1	understanding					2392:2404	The molecular understanding	2378:2404	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps	2378:2484	The molecular understanding of similarities and differences between hydrolysis and transglycosylation steps may be of help in the design of new biocatalysts for glycan synthesis.					
31024890	2	106	dep	advancement	227:237	arg1	both					256:259	both	256:259	both	256:259	The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level.					
31024890	10	107	theme	S	1461:1461	arg1	character					1465:1473	a stronger S 2 character	1450:1473	a stronger S 2 character than the deglycosylation ones	1450:1503	The glycosylation transition state has a stronger S 2 character than the deglycosylation ones and the proton transfer is less advanced.					
31024890	7	108	theme	QM	819:820	arg1	calculations					830:841	QM(DFT)/MM calculations	819:841	QM(DFT)/MM calculations	819:841	Here we have used QM(DFT)/MM calculations to compare the glycosylation, hydrolysis and transglycosylation steps catalyzed by wild type Thermus thermophilus β-glycosidase (family GH1), a retaining glycosyl hydrolase for which a transglycosylation yield of 36% has been determined experimentally.					
31756805	1	0	theme	leaves	251:256	arg1	potential					170:178	the methane potential	158:178	the methane potential	158:178	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	0	theme	leaves	251:256	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	6	1	theme	methane	1195:1201	arg1	production					1203:1212	methane production	1195:1212	methane production of different substrate	1195:1235	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	6	2	theme	substrate	1227:1235	arg1	production					1203:1212	methane production	1195:1212	methane production of different substrate	1195:1235	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	1	3	theme	stem	215:218	arg1	bark					220:223	the stem bark	211:223	the stem bark (SB)	211:228	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	3	theme	stem	215:218	arg1	SB					226:227	SB	226:227	SB	226:227	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	4	4	from	rates	911:915	arg1	substrate					964:972	the specific substrate	951:972	the specific substrate	951:972	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	1	5	theme	bark	220:223	arg1	potential					170:178	the methane potential	158:178	the methane potential	158:178	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	5	theme	bark	220:223	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	4	6	theme	soluble	811:817	arg1	compounds					819:827	soluble compounds	811:827	soluble compounds	811:827	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	5	7	theme	methane	1007:1013	arg1	production					1015:1024	The methane production	1003:1024	The methane production	1003:1024	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	7	8	theme	methane	1346:1352	arg1	yields					1354:1359	methane yields	1346:1359	methane yields	1346:1359	The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields.					
31756805	4	9	theme	hemicellulose	920:932	arg1	complex					979:985	complex	979:985	complex	979:985	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	4	9	theme	hemicellulose	920:932	arg1	rates					911:915	the degradation rates	895:915	the degradation rates of hemicellulose and cellulose in the specific substrate	895:972	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	5	10	theme	substrates	1086:1095	arg1	structure					1069:1077	the crystalline structure	1053:1077	the crystalline structure of the substrates	1053:1095	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	1	11	from	digestion	299:307	arg1	potential					170:178	the methane potential	158:178	the methane potential	158:178	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	11	from	digestion	299:307	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	3	12	theme	substrates	573:582	arg1	structure					556:564	the crystalline structure	540:564	the crystalline structure of the substrates as well as their changes during the anaerobic digestion	540:638	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	3	12	theme	substrates	573:582	arg1	composition					524:534	The chemical composition	511:534	The chemical composition	511:534	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	4	13	theme	cellulose	861:869	arg1	content					882:888	the cellulose and lignin content	857:888	content	882:888	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	4	14	theme	lignin	875:880	arg1	content					882:888	the cellulose and lignin content	857:888	content	882:888	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	0	15	theme	stem	104:107	arg1	bark					109:112	the stem bark	100:112	the stem bark	100:112	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	6	16	theme	functional	1154:1163	arg1	microorganism					1165:1177	functional microorganism	1154:1177	functional microorganism contributing to methane production of different substrate	1154:1235	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	7	17	theme	Bacteroidetes	1255:1267	arg1	affected					1293:1300	affected	1293:1300	most affected by the substrate	1288:1317	The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields.					
31756805	7	17	theme	Bacteroidetes	1255:1267	arg1	abundance					1242:1250	The abundance	1238:1250	The abundance of Bacteroidetes and Firmicutes	1238:1282	The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields.					
31756805	4	18	theme	compounds	819:827	arg1	content					829:835	the hemicellulose and soluble compounds content	789:835	the hemicellulose and soluble compounds content	789:835	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	0	19	from	digestion	38:46	arg1	production					8:17	Methane production	0:17	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.	0:136	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	5	20	theme	crystalline	1057:1067	arg1	structure					1069:1077	the crystalline structure	1053:1077	the crystalline structure of the substrates	1053:1095	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	6	21	theme	different	1217:1225	arg1	substrate					1227:1235	different substrate	1217:1235	different substrate	1217:1235	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	0	22	theme	Methane	0:6	arg1	production					8:17	Methane production	0:17	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.	0:136	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	1	23	theme	batch	283:287	arg1	digestion					299:307	batch anaerobic digestion	283:307	batch anaerobic digestion	283:307	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	4	24	theme	degradation	899:909	arg1	complex					979:985	complex	979:985	complex	979:985	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	4	24	theme	degradation	899:909	arg1	rates					911:915	the degradation rates	895:915	the degradation rates of hemicellulose and cellulose in the specific substrate	895:972	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	3	25	theme	changes	601:607	arg1	structure					556:564	the crystalline structure	540:564	the crystalline structure of the substrates as well as their changes during the anaerobic digestion	540:638	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	3	25	theme	changes	601:607	arg1	composition					524:534	The chemical composition	511:534	The chemical composition	511:534	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	1	26	theme	methane	162:168	arg1	potential					170:178	the methane potential	158:178	the methane potential	158:178	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	2	27	theme	methane	334:340	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	27	theme	methane	334:340	arg1	potential					342:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	1	28	theme	stem	231:234	arg1	SP					242:243	SP	242:243	SP	242:243	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	28	theme	stem	231:234	arg1	pith					236:239	stem pith	231:239	stem pith (SP)	231:244	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	3	29	from	impacts	666:672	arg1	characteristics					681:695	the characteristics	677:695	the characteristics of methane production	677:717	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	2	30	theme	obtained	314:321	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	30	theme	obtained	314:321	arg1	potential					342:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	1	31	theme	pith	236:239	arg1	potential					170:178	the methane potential	158:178	the methane potential	158:178	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	31	theme	pith	236:239	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	4	32	theme	hemicellulose	793:805	arg1	content					829:835	the hemicellulose and soluble compounds content	789:835	the hemicellulose and soluble compounds content	789:835	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	0	33	from	stover	72:77	arg1	digestion					38:46	the anaerobic digestion	24:46	the anaerobic digestion of substrates from corn stover	24:77	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	0	34	theme	anaerobic	28:36	arg1	digestion					38:46	the anaerobic digestion	24:46	the anaerobic digestion of substrates from corn stover	24:77	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	3	35	theme	anaerobic	620:628	arg1	digestion					630:638	the anaerobic digestion	616:638	the anaerobic digestion	616:638	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	0	36	theme	stem	115:118	arg1	pith					120:123	stem pith	115:123	stem pith	115:123	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	1	37	theme	anaerobic	289:297	arg1	digestion					299:307	batch anaerobic digestion	283:307	batch anaerobic digestion	283:307	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	2	38	theme	volatile	428:435	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	38	theme	volatile	428:435	arg1	solids					437:442	volatile solids	428:442	volatile solids	428:442	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	1	39	theme	methane	184:190	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	0	40	theme	substrates	51:60	arg1	digestion					38:46	the anaerobic digestion	24:46	the anaerobic digestion of substrates from corn stover	24:77	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	4	41	theme	production	746:755	arg1	rate					757:760	The methane production rate	734:760	The methane production rate	734:760	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	6	42	theme	community	1108:1116	arg1	structure					1118:1126	Microbial community structure	1098:1126	Microbial community structure	1098:1126	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	1	43	theme	corn	266:269	arg1	stover					271:276	corn stover	266:276	corn stover	266:276	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	2	44	theme	hydrolysis	360:369	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	44	theme	hydrolysis	360:369	arg1	constant					380:387	the hydrolysis kinetics constant	356:387	the hydrolysis kinetics constant	356:387	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	5	45	theme	limit	1030:1034	arg1	correlation					1036:1046	limit correlation	1030:1046	limit correlation	1030:1046	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	4	46	theme	methane	738:744	arg1	rate					757:760	The methane production rate	734:760	The methane production rate	734:760	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	3	47	theme	chemical	515:522	arg1	composition					524:534	The chemical composition	511:534	The chemical composition	511:534	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	6	48	theme	Microbial	1098:1106	arg1	structure					1118:1126	Microbial community structure	1098:1126	Microbial community structure	1098:1126	Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate.					
31756805	3	49	theme	methane	700:706	arg1	production					708:717	methane production	700:717	methane production	700:717	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	5	50	contain	has	1026:1028	arg1	production					1015:1024	The methane production	1003:1024	The methane production	1003:1024	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	5	50	contain	has	1026:1028	arg2	correlation					1036:1046	limit correlation	1030:1046	limit correlation	1030:1046	The methane production has limit correlation with the crystalline structure of the substrates.					
31756805	7	51	theme	Firmicutes	1273:1282	arg1	affected					1293:1300	affected	1293:1300	most affected by the substrate	1288:1317	The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields.					
31756805	7	51	theme	Firmicutes	1273:1282	arg1	abundance					1242:1250	The abundance	1238:1250	The abundance of Bacteroidetes and Firmicutes	1238:1282	The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields.					
31756805	1	52	from	potential	170:178	arg1	digestion					299:307	batch anaerobic digestion	283:307	batch anaerobic digestion	283:307	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	3	53	theme	production	708:717	arg1	characteristics					681:695	the characteristics	677:695	the characteristics of methane production	677:717	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31756805	1	54	theme	production	192:201	arg1	rate					203:206	methane production rate	184:206	methane production rate	184:206	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	1	55	from	rate	203:206	arg1	digestion					299:307	batch anaerobic digestion	283:307	batch anaerobic digestion	283:307	This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion.					
31756805	4	56	theme	specific	955:962	arg1	substrate					964:972	the specific substrate	951:972	the specific substrate	951:972	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	0	57	theme	corn	67:70	arg1	stover					72:77	corn stover	67:77	corn stover	67:77	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	0	58	dep	production	8:17	arg1	Differences					80:90	Differences	80:90	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.	0:136	Methane production from the anaerobic digestion of substrates from corn stover: Differences between the stem bark, stem pith, and leaves.					
31756805	2	59	theme	cumulative	323:332	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	59	theme	cumulative	323:332	arg1	potential					342:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential	310:350	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	4	60	theme	cellulose	938:946	arg1	complex					979:985	complex	979:985	complex	979:985	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	4	60	theme	cellulose	938:946	arg1	rates					911:915	the degradation rates	895:915	the degradation rates of hemicellulose and cellulose in the specific substrate	895:972	The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable.					
31756805	2	61	theme	kinetics	371:378	arg1	0.201					394:398	0.201	394:398	0.201	394:398	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	2	61	theme	kinetics	371:378	arg1	constant					380:387	the hydrolysis kinetics constant	356:387	the hydrolysis kinetics constant	356:387	The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g-1 VS (volatile solids) and 0.090, 0.149, and 0.227 d-1 for SB, SP, and LV, respectively.					
31756805	3	62	theme	crystalline	544:554	arg1	structure					556:564	the crystalline structure	540:564	the crystalline structure of the substrates as well as their changes during the anaerobic digestion	540:638	The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed.					
31733242	4	0	attach	attached	637:644	arg2	Xylp					613:616	Xylp, and Araf residues	613:635	Xylp	613:616	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	4	0	attach	attached	637:644	arg3	positions					661:669	O-2 and O-3 positions	649:669	positions	661:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	4	1	theme	Araf	623:626	arg1	residues					628:635	Xylp, and Araf residues	613:635	residues	628:635	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	2	2	theme	extraction	353:362	arg1	%					396:396	close to 18.6%	383:396	close to 18.6% (w/v)	383:402	The final extraction yield for PSPC was close to 18.6% (w/v).					
31733242	2	2	theme	extraction	353:362	arg1	yield					364:368	The final extraction yield	343:368	The final extraction yield for PSPC	343:377	The final extraction yield for PSPC was close to 18.6% (w/v).					
31733242	7	3	theme	current	1036:1042	arg1	research					1044:1051	This current research	1031:1051	This current research	1031:1051	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	1	4	theme	Algerian	238:245	arg1	Desf.					217:221	Desf.	217:221	Desf.	217:221	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	1	4	theme	Algerian	238:245	arg1	plant					255:259	a spontaneous Algerian Saharan plant	224:259	a spontaneous Algerian Saharan plant	224:259	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	5	5	theme	polydispersity	805:818	arg1	PDI					827:829	PDI	827:829	PDI	827:829	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	5	theme	polydispersity	805:818	arg1	index					820:824	a low polydispersity index	799:824	a low polydispersity index (PDI) of 1.47	799:838	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	6	from	PSPC	710:713	arg1	water					718:722	water	718:722	water	718:722	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	2	7	theme	final	347:351	arg1	%					396:396	close to 18.6%	383:396	close to 18.6% (w/v)	383:402	The final extraction yield for PSPC was close to 18.6% (w/v).					
31733242	2	7	theme	final	347:351	arg1	yield					364:368	The final extraction yield	343:368	The final extraction yield for PSPC	343:377	The final extraction yield for PSPC was close to 18.6% (w/v).					
31733242	1	8	theme	Saharan	247:253	arg1	Desf.					217:221	Desf.	217:221	Desf.	217:221	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	1	8	theme	Saharan	247:253	arg1	plant					255:259	a spontaneous Algerian Saharan plant	224:259	a spontaneous Algerian Saharan plant	224:259	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	6	9	theme	overlay	965:971	arg1	concentration					973:985	the critical overlay concentration	952:985	the critical overlay concentration (C*)	952:990	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	6	9	theme	overlay	965:971	arg1	*					989:989	C*	988:989	C*	988:989	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	5	10	theme	700 kDa	790:796	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	10	theme	700 kDa	790:796	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	11	with	SEC/MALLS	742:750	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	11	with	SEC/MALLS	742:750	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	4	12	theme	O-2	649:651	arg1	positions					661:669	O-2 and O-3 positions	649:669	positions	661:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	4	13	theme	β-	560:561	arg1	backbone					584:591	a β-(1,3)/β-(1,4)-d-xylan backbone	558:591	a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	558:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	0	14	theme	ciliata	116:122	arg1	Desf					124:127	Plantago ciliata Desf	107:127	Plantago ciliata Desf	107:127	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	7	15	theme	new	1103:1105	arg1	useful					1148:1153	useful	1148:1153	useful	1148:1153	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	7	15	theme	new	1103:1105	arg1	arabinoxylan					1107:1118	a new arabinoxylan	1101:1118	a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium	1101:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	7	16	theme	novel	1160:1164	arg1	source					1166:1171	a novel source	1158:1171	a novel source of soluble fiber belonging to psyllium	1158:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	5	17	theme	intrinsic	847:855	arg1	viscosity					857:865	an intrinsic viscosity	844:865	an intrinsic viscosity [η] close to 157 mL/g	844:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	18	theme	index	820:824	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	18	theme	index	820:824	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	4	19	theme	side	598:601	arg1	chains					603:608	side chains	598:608	side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	598:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	0	20	theme	Plantago	107:114	arg1	Desf					124:127	Plantago ciliata Desf	107:127	Plantago ciliata Desf	107:127	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	5	21	theme	viscosity	857:865	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	21	theme	viscosity	857:865	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	6	22	theme	critical	956:963	arg1	concentration					973:985	the critical overlay concentration	952:985	the critical overlay concentration (C*)	952:990	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	6	22	theme	critical	956:963	arg1	*					989:989	C*	988:989	C*	988:989	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	7	23	theme	fiber	1184:1188	arg1	source					1166:1171	a novel source	1158:1171	a novel source of soluble fiber belonging to psyllium	1158:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	7	24	theme	original	1066:1073	arg1	information					1086:1096	original structural information	1066:1096	original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium	1066:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	4	25	theme	O-3	657:659	arg1	positions					661:669	O-2 and O-3 positions	649:669	positions	661:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	0	26	theme	Structural	0:9	arg1	features					11:18	Structural features	0:18	Structural features	0:18	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	7	27	from	information	1086:1096	arg1	useful					1148:1153	useful	1148:1153	useful	1148:1153	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	7	27	from	information	1086:1096	arg1	arabinoxylan					1107:1118	a new arabinoxylan	1101:1118	a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium	1101:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	5	28	from	characteristics	691:705	arg1	water					718:722	water	718:722	water	718:722	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	6	29	theme	semi-dilute	930:940	arg1	media					942:946	semi-dilute media	930:946	semi-dilute media	930:946	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	0	30	theme	rheological	24:34	arg1	behavior					36:43	rheological behavior	24:43	rheological behavior	24:43	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	1	31	theme	hot	266:268	arg1	extraction					278:287	a hot aqueous extraction	264:287	a hot aqueous extraction then purified by successive ethanolic precipitations	264:340	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	6	32	from	behavior	918:925	arg1	media					942:946	semi-dilute media	930:946	semi-dilute media	930:946	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	6	33	theme	C	988:988	arg1	concentration					973:985	the critical overlay concentration	952:985	the critical overlay concentration (C*)	952:990	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	6	33	theme	C	988:988	arg1	*					989:989	C*	988:989	C*	988:989	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	1	34	theme	aqueous	270:276	arg1	extraction					278:287	a hot aqueous extraction	264:287	a hot aqueous extraction then purified by successive ethanolic precipitations	264:340	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	0	35	theme	Desf	124:127	arg1	seeds					98:102	the seeds	94:102	the seeds of Plantago ciliata Desf	94:127	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	5	36	theme	1.47	835:838	arg1	PDI					827:829	PDI	827:829	PDI	827:829	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	36	theme	1.47	835:838	arg1	index					820:824	a low polydispersity index	799:824	a low polydispersity index (PDI) of 1.47	799:838	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	36	theme	1.47	835:838	arg1	700 kDa					790:796	700 kDa	790:796	700 kDa	790:796	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	36	theme	1.47	835:838	arg1	viscosity					857:865	an intrinsic viscosity	844:865	an intrinsic viscosity [η] close to 157 mL/g	844:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	4	37	theme	1,4	571:573	arg1	backbone					584:591	a β-(1,3)/β-(1,4)-d-xylan backbone	558:591	a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	558:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	5	38	theme	PSPC	710:713	arg1	characteristics					691:705	The macromolecular characteristics	672:705	The macromolecular characteristics of PSPC in water	672:722	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	4	39	theme	residues	628:635	arg1	chains					603:608	side chains	598:608	side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	598:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	6	40	theme	pseudoplastic	904:916	arg1	behavior					918:925	a pseudoplastic behavior	902:925	a pseudoplastic behavior in semi-dilute media	902:946	PSPC showed a pseudoplastic behavior in semi-dilute media and the critical overlay concentration (C*) was estimated around 0.32-0.37% (w/v).					
31733242	5	41	theme	high	760:763	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	41	theme	high	760:763	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	7	42	theme	soluble	1176:1182	arg1	fiber					1184:1188	soluble fiber	1176:1188	soluble fiber belonging to psyllium	1176:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	2	43	dep	18.6	392:395	arg1	to					389:390	to	389:390	to	389:390	The final extraction yield for PSPC was close to 18.6% (w/v).					
31733242	4	44	theme	-d-xylan	575:582	arg1	backbone					584:591	a β-(1,3)/β-(1,4)-d-xylan backbone	558:591	a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	558:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	0	45	theme	water-soluble	50:62	arg1	polysaccharide					64:77	a water-soluble polysaccharide	48:77	a water-soluble polysaccharide	48:77	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	4	46	theme	neutral	524:530	arg1	arabinoxylan					532:543	a neutral arabinoxylan	522:543	a neutral arabinoxylan	522:543	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	4	46	theme	neutral	524:530	arg1	PSPC					514:517	PSPC	514:517	PSPC	514:517	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	5	47	theme	low	801:803	arg1	PDI					827:829	PDI	827:829	PDI	827:829	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	47	theme	low	801:803	arg1	index					820:824	a low polydispersity index	799:824	a low polydispersity index (PDI) of 1.47	799:838	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	48	from	water	718:722	arg1	characteristics					691:705	The macromolecular characteristics	672:705	The macromolecular characteristics of PSPC in water	672:722	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	4	49	with	backbone	584:591	arg1	chains					603:608	side chains	598:608	side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	598:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	3	50	theme	structural	466:475	arg1	features					477:484	structural features	466:484	structural features	466:484	PSPC was then investigated regarding its global composition, structural features and rheological properties.					
31733242	1	51	theme	successive	306:315	arg1	precipitations					327:340	successive ethanolic precipitations	306:340	successive ethanolic precipitations	306:340	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	5	52	theme	[η	867:868	arg1	157 mL/g					880:887	157 mL/g	880:887	157 mL/g	880:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	53	theme	macromolecular	676:689	arg1	characteristics					691:705	The macromolecular characteristics	672:705	The macromolecular characteristics of PSPC in water	672:722	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	3	54	theme	global	446:451	arg1	composition					453:463	its global composition	442:463	its global composition	442:463	PSPC was then investigated regarding its global composition, structural features and rheological properties.					
31733242	1	55	theme	Desf.	217:221	arg1	seeds					191:195	the seeds	187:195	the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant	187:259	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	1	56	theme	water-soluble	132:144	arg1	PSPC					162:165	PSPC	162:165	PSPC	162:165	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	1	56	theme	water-soluble	132:144	arg1	polysaccharide					146:159	A water-soluble polysaccharide	130:159	A water-soluble polysaccharide (PSPC)	130:166	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	4	57	theme	1,3	563:565	arg1	backbone					584:591	a β-(1,3)/β-(1,4)-d-xylan backbone	558:591	a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	558:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	7	58	theme	structural	1075:1084	arg1	information					1086:1096	original structural information	1066:1096	original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium	1066:1210	This current research has supplied original structural information on a new arabinoxylan which could be particularly useful as a novel source of soluble fiber belonging to psyllium.					
31733242	5	59	theme	molecular	765:773	arg1	Mw					783:784	Mw	783:784	Mw	783:784	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	5	59	theme	molecular	765:773	arg1	weight					775:780	a high molecular weight	758:780	a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g	758:887	The macromolecular characteristics of PSPC in water was determined by SEC/MALLS, with a high molecular weight (Mw) of 700 kDa, a low polydispersity index (PDI) of 1.47 and an intrinsic viscosity [η] close to 157 mL/g.					
31733242	3	60	theme	rheological	490:500	arg1	properties					502:511	rheological properties	490:511	rheological properties	490:511	PSPC was then investigated regarding its global composition, structural features and rheological properties.					
31733242	0	61	theme	polysaccharide	64:77	arg1	features					11:18	Structural features	0:18	Structural features	0:18	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	0	61	theme	polysaccharide	64:77	arg1	behavior					36:43	rheological behavior	24:43	rheological behavior	24:43	Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf.					
31733242	1	62	theme	ethanolic	317:325	arg1	precipitations					327:340	successive ethanolic precipitations	306:340	successive ethanolic precipitations	306:340	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	4	63	theme	/β-	567:569	arg1	backbone					584:591	a β-(1,3)/β-(1,4)-d-xylan backbone	558:591	a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	558:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	4	64	theme	Xylp	613:616	arg1	chains					603:608	side chains	598:608	side chains of Xylp, and Araf residues attached in O-2 and O-3 positions	598:669	PSPC is a neutral arabinoxylan, composed of a β-(1,3)/β-(1,4)-d-xylan backbone with side chains of Xylp, and Araf residues attached in O-2 and O-3 positions.					
31733242	1	65	theme	spontaneous	226:236	arg1	Desf.					217:221	Desf.	217:221	Desf.	217:221	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
31733242	1	65	theme	spontaneous	226:236	arg1	plant					255:259	a spontaneous Algerian Saharan plant	224:259	a spontaneous Algerian Saharan plant	224:259	A water-soluble polysaccharide (PSPC) was extracted from the seeds of Plantago ciliata Desf., a spontaneous Algerian Saharan plant by a hot aqueous extraction then purified by successive ethanolic precipitations.					
29660454	3	0	dep	together	565:572	arg1	with					574:577	with	574:577	with	574:577	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	4	1	theme	molar	708:712	arg1	ratio					714:718	the molar ratio	704:718	the molar ratio of 1:0.098	704:729	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	0	2	theme	fruiting	83:90	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of the Grifola frondosa	79:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	3	3	theme	2D	611:612	arg1	NMR					614:616	2D NMR	611:616	2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra)	611:660	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	0	4	from	Purification	0:11	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of the Grifola frondosa	79:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	4	5	from	D-Gal	695:699	arg1	ratio					714:718	the molar ratio	704:718	the molar ratio of 1:0.098	704:729	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	2	6	theme	water-soluble	278:290	arg1	polysaccharide					292:305	A water-soluble polysaccharide	276:305	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da)	276:355	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	4	7	theme	‑α‑d‑Glcp‑	823:832	arg1	‑α‑d‑Glcp					840:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	4	7	theme	‑α‑d‑Glcp‑	823:832	arg1	structure					739:747	the structure	735:747	the structure of the repeating units	735:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	3	8	theme	methylation	543:553	arg1	analysis					555:562	methylation analysis	543:562	methylation analysis	543:562	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	1	9	theme	medicinal	256:264	arg1	mushroom					266:273	a medicinal mushroom	254:273	a medicinal mushroom	254:273	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	0	10	theme	frondosa	114:121	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of the Grifola frondosa	79:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	2	11	theme	G.	398:399	arg1	frondosa					401:408	G. frondosa	398:408	G. frondosa	398:408	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	3	12	theme	monosaccharide	515:528	arg1	composition					530:540	its monosaccharide composition	511:540	its monosaccharide composition	511:540	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	2	13	theme	fruiting	379:386	arg1	bodies					388:393	the fruiting bodies	375:393	the fruiting bodies of G. frondosa	375:408	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	0	14	theme	Grifola	106:112	arg1	frondosa					114:121	the Grifola frondosa	102:121	the Grifola frondosa	102:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	3	15	dep	NMR	582:584	arg1	NMR					590:592	1H NMR	587:592	1H NMR	587:592	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	15	dep	NMR	582:584	arg1	NMR					602:604	13C NMR	598:604	13C NMR	598:604	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	16	theme	NOESY	647:651	arg1	spectra					653:659	NOESY spectra	647:659	NOESY spectra	647:659	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	4	17	theme	units	766:770	arg1	‑α‑d‑Glcp					840:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	4	17	theme	units	766:770	arg1	structure					739:747	the structure	735:747	the structure of the repeating units	735:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	2	18	dep	polysaccharide	292:305	arg1	weight					337:342	molecular weight	327:342	molecular weight: 2.04×106Da	327:354	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	2	19	theme	water	417:421	arg1	extraction					423:432	hot water extraction	413:432	hot water extraction	413:432	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	4	20	theme	repeating	756:764	arg1	units					766:770	the repeating units	752:770	the repeating units	752:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	2	21	dep	GFP30-2-a	316:324	arg1	code					308:311	code	308:311	code	308:311	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	2	22	theme	hot	413:415	arg1	extraction					423:432	hot water extraction	413:432	hot water extraction	413:432	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	4	23	from	D-Glc	685:689	arg1	ratio					714:718	the molar ratio	704:718	the molar ratio of 1:0.098	704:729	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	2	24	dep	weight	337:342	arg1	2.04×106Da					345:354	2.04×106Da	345:354	molecular weight: 2.04×106Da	327:354	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	2	25	theme	frondosa	401:408	arg1	bodies					388:393	the fruiting bodies	375:393	the fruiting bodies of G. frondosa	375:408	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	0	26	theme	structural	17:26	arg1	elucidation					28:38	structural elucidation	17:38	structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa	17:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	4	27	theme	‑α‑d‑Glcp‑	808:817	arg1	‑α‑d‑Glcp					840:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	4	27	theme	‑α‑d‑Glcp‑	808:817	arg1	structure					739:747	the structure	735:747	the structure of the repeating units	735:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	0	28	from	bodies	92:97	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	0	28	from	bodies	92:97	arg1	elucidation					28:38	structural elucidation	17:38	structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa	17:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	0	28	from	bodies	92:97	arg1	polysaccharide					59:72	a water-soluble polysaccharide	43:72	a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa	43:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	1	29	theme	many	189:192	arg1	centuries					194:202	many centuries	189:202	many centuries	189:202	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	2	30	theme	molecular	327:335	arg1	weight					337:342	molecular weight	327:342	molecular weight: 2.04×106Da	327:354	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	3	31	theme	1H	587:588	arg1	NMR					590:592	1H NMR	587:592	1H NMR	587:592	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	0	32	theme	polysaccharide	59:72	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	0	32	theme	polysaccharide	59:72	arg1	elucidation					28:38	structural elucidation	17:38	structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa	17:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	4	33	theme	m→4	836:838	arg1	‑α‑d‑Glcp					840:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	4	33	theme	m→4	836:838	arg1	structure					739:747	the structure	735:747	the structure of the repeating units	735:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	3	34	theme	1D	579:580	arg1	NMR					582:584	1D NMR	579:584	1D NMR (1H NMR and 13C NMR)	579:605	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	0	35	theme	water-soluble	45:57	arg1	polysaccharide					59:72	a water-soluble polysaccharide	43:72	a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa	43:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	4	36	theme	β‑d‑Glcp‑	793:801	arg1	‑α‑d‑Glcp					840:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp	793:848	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	4	36	theme	β‑d‑Glcp‑	793:801	arg1	structure					739:747	the structure	735:747	the structure of the repeating units	735:770	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	1	37	theme	Grifola	124:130	arg1	frondosa					132:139	Grifola frondosa	124:139	Grifola frondosa	124:139	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	1	37	theme	Grifola	124:130	arg1	mushroom					155:162	a polypore mushroom	144:162	a polypore mushroom	144:162	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	3	38	theme	13C	598:600	arg1	NMR					602:604	13C NMR	598:604	13C NMR	598:604	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	1	39	used	used	180:183	arg2	mushroom					155:162	a polypore mushroom	144:162	a polypore mushroom	144:162	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	1	39	used	used	180:183	arg2	frondosa					132:139	Grifola frondosa	124:139	Grifola frondosa	124:139	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	4	40	theme	1:0.098	723:729	arg1	ratio					714:718	the molar ratio	704:718	the molar ratio of 1:0.098	704:729	GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.					
29660454	1	41	theme	traditional	207:217	arg1	herbalists					240:249	traditional Chinese and Japanese herbalists	207:249	traditional Chinese and Japanese herbalists as a medicinal mushroom	207:273	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	2	42	attach	isolated	361:368	arg2	polysaccharide					292:305	A water-soluble polysaccharide	276:305	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da)	276:355	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	2	42	attach	isolated	361:368	arg1	bodies					388:393	the fruiting bodies	375:393	the fruiting bodies of G. frondosa	375:408	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	1	43	theme	Chinese	219:225	arg1	herbalists					240:249	traditional Chinese and Japanese herbalists	207:249	traditional Chinese and Japanese herbalists as a medicinal mushroom	207:273	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	2	44	theme	ethanol	435:441	arg1	precipitation					443:455	ethanol precipitation	435:455	ethanol precipitation	435:455	A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography.					
29660454	0	45	from	elucidation	28:38	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of the Grifola frondosa	79:121	Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.					
29660454	3	46	dep	NMR	614:616	arg1	COSY					619:622	COSY	619:622	COSY	619:622	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	46	dep	NMR	614:616	arg1	HMBC					638:641	HMBC	638:641	HMBC	638:641	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	46	dep	NMR	614:616	arg1	spectra					653:659	NOESY spectra	647:659	NOESY spectra	647:659	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	46	dep	NMR	614:616	arg1	HSQC					632:635	HSQC	632:635	HSQC	632:635	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	3	46	dep	NMR	614:616	arg1	TOCSY					625:629	TOCSY	625:629	TOCSY	625:629	Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra).					
29660454	1	47	theme	polypore	146:153	arg1	frondosa					132:139	Grifola frondosa	124:139	Grifola frondosa	124:139	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	1	47	theme	polypore	146:153	arg1	mushroom					155:162	a polypore mushroom	144:162	a polypore mushroom	144:162	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
29660454	1	48	theme	Japanese	231:238	arg1	herbalists					240:249	traditional Chinese and Japanese herbalists	207:249	traditional Chinese and Japanese herbalists as a medicinal mushroom	207:273	Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom.					
31216303	16	0	theme	flocculation	1836:1847	arg1	functionality					1849:1861	no flocculation functionality	1833:1861	no flocculation functionality	1833:1861	On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality.					
31216303	4	1	from	isolates	925:932	arg1	polysaccharides					874:888	extracellular polysaccharides	860:888	extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components	860:964	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	1	from	isolates	925:932	arg1	EPS					891:893	EPS	891:893	EPS	891:893	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	2	theme	microbial	745:753	arg1	structure					765:773	its microbial community structure	741:773	its microbial community structure	741:773	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	5	3	theme	high	1001:1004	arg1	biodiversity					1016:1027	high bacterial biodiversity	1001:1027	high bacterial biodiversity	1001:1027	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	3	theme	high	1001:1004	arg1	phyla					1105:1109	the most abundant phyla	1087:1109	the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter	1087:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	3	theme	high	1001:1004	arg1	genera					1214:1219	the dominant genera	1201:1219	the dominant genera	1201:1219	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	0	4	theme	philippinarum	110:122	arg1	resource					88:95	a promising natural bioflocculant resource	54:95	a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	54:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	4	5	from	components	955:964	arg1	polysaccharides					874:888	extracellular polysaccharides	860:888	extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components	860:964	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	5	from	components	955:964	arg1	EPS					891:893	EPS	891:893	EPS	891:893	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	17	6	theme	bacterial	1951:1959	arg1	origin					1961:1966	bacterial origin	1951:1966	bacterial origin	1951:1966	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	15	7	theme	similar	1669:1675	arg1	composition					1692:1702	highly similar monosaccharide composition	1662:1702	highly similar monosaccharide composition	1662:1702	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	15	8	theme	bioflocculant-producing	1620:1642	arg1	strains					1644:1650	the bioflocculant-producing strains	1616:1650	the bioflocculant-producing strains	1616:1650	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	8	9	dep	sp	1389:1390	arg1	3					1375:1375	3	1375:1375	3	1375:1375	(3), Halomonas sp.					
31216303	14	10	theme	flocculation	1569:1580	arg1	capability					1582:1591	their excellent flocculation capability	1553:1591	their excellent flocculation capability	1553:1591	were reported for the first time for their excellent flocculation capability.					
31216303	3	11	theme	RPM	583:585	arg1	components					603:612	RPM bioflocculation components	583:612	RPM bioflocculation components	583:612	This study investigated the probability that RPM bioflocculation components originate from its associated microbes.					
31216303	17	12	from	RPM	1887:1889	arg1	Findings					1864:1871	Findings	1864:1871	Findings from Zhoushan RPM	1864:1889	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	1	13	theme	promising	371:379	arg1	philippinarum					198:210	Ruditapes philippinarum	188:210	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	1	13	theme	promising	371:379	arg1	resource					403:410	a promising natural bioflocculant resource	369:410	a promising natural bioflocculant resource	369:410	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	4	14	theme	aquaculture	674:684	arg1	farm					686:689	an aquaculture farm	671:689	an aquaculture farm in Zhoushan of China	671:710	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	0	15	dep	philippinarum	110:122	arg1	mud					139:141	conglutination mud	124:141	Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	100:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	0	16	theme	conglutination	124:137	arg1	mud					139:141	conglutination mud	124:141	Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	100:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	1	17	theme	natural	381:387	arg1	philippinarum					198:210	Ruditapes philippinarum	188:210	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	1	17	theme	natural	381:387	arg1	resource					403:410	a promising natural bioflocculant resource	369:410	a promising natural bioflocculant resource	369:410	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	6	18	theme	Pseudoalteromonas	1329:1345	arg1	sp					1347:1348	Pseudoalteromonas sp	1329:1348	Pseudoalteromonas sp	1329:1348	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	6	18	theme	Pseudoalteromonas	1329:1345	arg1	strains					1272:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	1	19	theme	bioflocculant	389:401	arg1	philippinarum					198:210	Ruditapes philippinarum	188:210	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	1	19	theme	bioflocculant	389:401	arg1	resource					403:410	a promising natural bioflocculant resource	369:410	a promising natural bioflocculant resource	369:410	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	4	20	theme	flocculation	942:953	arg1	components					955:964	RPM flocculation components	938:964	RPM flocculation components	938:964	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	9	21	dep	sp	1414:1415	arg1	2					1394:1394	2	1394:1394	2	1394:1394	(2), Albirhodobacter sp.					
31216303	9	21	dep	sp	1414:1415	arg1	Albirhodobacter					1398:1412	Albirhodobacter	1398:1412	Albirhodobacter	1398:1412	(2), Albirhodobacter sp.					
31216303	4	22	theme	bioflocculant-producing	794:816	arg1	strains					818:824	associated bioflocculant-producing strains	783:824	associated bioflocculant-producing strains	783:824	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	17	23	theme	RPM	1915:1917	arg1	components					1932:1941	RPM flocculation components	1915:1941	RPM flocculation components	1915:1941	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	0	24	theme	Microbial	0:8	arg1	origin					10:15	Microbial origin	0:15	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.	0:186	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	1	25	theme	important	279:287	arg1	mollusk					310:316	an important commercially bivalve mollusk	276:316	an important commercially bivalve mollusk R. philippinarum	276:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	4	26	theme	RPM	938:940	arg1	components					955:964	RPM flocculation components	938:964	RPM flocculation components	938:964	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	15	27	theme	RPM	1743:1745	arg1	polysaccharides					1747:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	4	28	theme	associated	783:792	arg1	strains					818:824	associated bioflocculant-producing strains	783:824	associated bioflocculant-producing strains	783:824	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	10	29	dep	sp	1436:1437	arg1	1					1419:1419	1	1419:1419	1	1419:1419	(1), Celeribacter sp.					
31216303	10	29	dep	sp	1436:1437	arg1	Celeribacter					1423:1434	Celeribacter	1423:1434	Celeribacter	1423:1434	(1), Celeribacter sp.					
31216303	1	30	theme	conglutination	212:225	arg1	mud					227:229	conglutination mud	212:229	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	0	31	theme	bioflocculation	20:34	arg1	components					36:45	bioflocculation components	20:45	bioflocculation components	20:45	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	7	32	dep	sp	1370:1371	arg1	5					1352:1352	5	1352:1352	5	1352:1352	(5), Psychrobacter sp.					
31216303	7	32	dep	sp	1370:1371	arg1	Psychrobacter					1356:1368	Psychrobacter	1356:1368	Psychrobacter	1356:1368	(5), Psychrobacter sp.					
31216303	14	33	theme	first	1538:1542	arg1	time					1544:1547	the first time	1534:1547	the first time for their excellent flocculation capability	1534:1591	were reported for the first time for their excellent flocculation capability.					
31216303	3	34	theme	bioflocculation	587:601	arg1	components					603:612	RPM bioflocculation components	583:612	RPM bioflocculation components	583:612	This study investigated the probability that RPM bioflocculation components originate from its associated microbes.					
31216303	2	35	theme	components	451:460	arg1	doubt					492:496	a pending doubt	482:496	a pending doubt	482:496	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	2	35	theme	components	451:460	arg1	origin					425:430	the origin	421:430	the origin of bioflocculation components within RPM	421:471	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	1	36	theme	bivalve	302:308	arg1	mollusk					310:316	an important commercially bivalve mollusk	276:316	an important commercially bivalve mollusk R. philippinarum	276:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	2	37	theme	pending	484:490	arg1	doubt					492:496	a pending doubt	482:496	a pending doubt	482:496	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	2	37	theme	pending	484:490	arg1	origin					425:430	the origin	421:430	the origin of bioflocculation components within RPM	421:471	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	2	38	theme	bioflocculation	435:449	arg1	components					451:460	bioflocculation components	435:460	bioflocculation components	435:460	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	1	39	theme	mollusk	310:316	arg1	philippinarum					321:333	an important commercially bivalve mollusk R. philippinarum	276:333	an important commercially bivalve mollusk R. philippinarum	276:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	16	40	from	existence	1787:1795	arg1	RPM					1809:1811	RPM	1809:1811	RPM	1809:1811	On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality.					
31216303	0	41	from	resource	88:95	arg1	farm					163:166	an aquaculture farm	148:166	an aquaculture farm in Zhoushan, China	148:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	1	42	dep	philippinarum	198:210	arg1	mud					227:229	conglutination mud	212:229	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	8	43	theme	Halomonas	1379:1387	arg1	sp					1389:1390	Halomonas sp	1379:1390	Halomonas sp	1379:1390	(3), Halomonas sp.					
31216303	14	44	theme	excellent	1559:1567	arg1	capability					1582:1591	their excellent flocculation capability	1553:1591	their excellent flocculation capability	1553:1591	were reported for the first time for their excellent flocculation capability.					
31216303	0	45	theme	components	36:45	arg1	origin					10:15	Microbial origin	0:15	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.	0:186	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	1	46	theme	R.	318:319	arg1	philippinarum					321:333	an important commercially bivalve mollusk R. philippinarum	276:333	an important commercially bivalve mollusk R. philippinarum	276:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	0	47	theme	aquaculture	151:161	arg1	farm					163:166	an aquaculture farm	148:166	an aquaculture farm in Zhoushan, China	148:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	5	48	theme	dominant	1205:1212	arg1	biodiversity					1016:1027	high bacterial biodiversity	1001:1027	high bacterial biodiversity	1001:1027	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	48	theme	dominant	1205:1212	arg1	genera					1214:1219	the dominant genera	1201:1219	the dominant genera	1201:1219	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	13	49	dep	all	1483:1485	arg1	1					1479:1479	1	1479:1479	1	1479:1479	(1), all of which except Bacillus sp.					
31216303	15	50	theme	reported	1711:1718	arg1	polysaccharides					1747:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	5	51	dep	phyla	1105:1109	arg1	CL500_29_marine_group					1124:1144	CL500_29_marine_group	1124:1144	CL500_29_marine_group	1124:1144	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	51	dep	phyla	1105:1109	arg1	Arcobacter					1187:1196	Arcobacter	1187:1196	Arcobacter	1187:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	51	dep	phyla	1105:1109	arg1	MWH_UniP1_aquatic_group					1159:1181	MWH_UniP1_aquatic_group	1159:1181	MWH_UniP1_aquatic_group	1159:1181	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	51	dep	phyla	1105:1109	arg1	phyla					1105:1109	the most abundant phyla	1087:1109	the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter	1087:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	0	52	theme	promising	56:64	arg1	resource					88:95	a promising natural bioflocculant resource	54:95	a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	54:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	1	53	theme	philippinarum	321:333	arg1	aquiculture					261:271	the aquiculture	257:271	the aquiculture of an important commercially bivalve mollusk R. philippinarum	257:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	2	54	theme	effective	514:522	arg1	exploitation					524:535	its effective exploitation	510:535	its effective exploitation	510:535	However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.					
31216303	4	55	theme	bioflocculant-producing	901:923	arg1	isolates					925:932	bioflocculant-producing isolates	901:932	bioflocculant-producing isolates	901:932	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	15	56	theme	flocculation-effective	1720:1741	arg1	polysaccharides					1747:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	the reported flocculation-effective RPM polysaccharides	1707:1761	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	5	57	theme	bacterial	1006:1014	arg1	biodiversity					1016:1027	high bacterial biodiversity	1001:1027	high bacterial biodiversity	1001:1027	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	57	theme	bacterial	1006:1014	arg1	phyla					1105:1109	the most abundant phyla	1087:1109	the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter	1087:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	57	theme	bacterial	1006:1014	arg1	genera					1214:1219	the dominant genera	1201:1219	the dominant genera	1201:1219	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	1	58	from	aquiculture	261:271	arg1	byproduct					242:250	a byproduct	240:250	a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum	240:333	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	1	58	from	aquiculture	261:271	arg1	philippinarum					198:210	Ruditapes philippinarum	188:210	Ruditapes philippinarum conglutination mud (RPM)	188:235	Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource.					
31216303	11	59	dep	sp	1453:1454	arg1	1					1441:1441	1	1441:1441	1	1441:1441	(1), Kocuria sp.					
31216303	11	59	dep	sp	1453:1454	arg1	Kocuria					1445:1451	Kocuria	1445:1451	Kocuria	1445:1451	(1), Kocuria sp.					
31216303	16	60	theme	other	1771:1775	arg1	hand					1777:1780	the other hand	1767:1780	the other hand	1767:1780	On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality.					
31216303	3	61	theme	associated	633:642	arg1	microbes					644:651	its associated microbes	629:651	its associated microbes	629:651	This study investigated the probability that RPM bioflocculation components originate from its associated microbes.					
31216303	4	62	from	farm	686:689	arg1	samples					658:664	RPM samples	654:664	RPM samples from an aquaculture farm in Zhoushan of China	654:710	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	62	from	farm	686:689	arg1	Zhoushan					694:701	Zhoushan	694:701	Zhoushan	694:701	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	63	theme	community	755:763	arg1	structure					765:773	its microbial community structure	741:773	its microbial community structure	741:773	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	17	64	theme	flocculation	1919:1930	arg1	components					1932:1941	RPM flocculation components	1915:1941	RPM flocculation components	1915:1941	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	5	65	theme	abundant	1096:1103	arg1	CL500_29_marine_group					1124:1144	CL500_29_marine_group	1124:1144	CL500_29_marine_group	1124:1144	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	65	theme	abundant	1096:1103	arg1	Arcobacter					1187:1196	Arcobacter	1187:1196	Arcobacter	1187:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	65	theme	abundant	1096:1103	arg1	biodiversity					1016:1027	high bacterial biodiversity	1001:1027	high bacterial biodiversity	1001:1027	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	65	theme	abundant	1096:1103	arg1	phyla					1105:1109	the most abundant phyla	1087:1109	the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter	1087:1196	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	5	65	theme	abundant	1096:1103	arg1	MWH_UniP1_aquatic_group					1159:1181	MWH_UniP1_aquatic_group	1159:1181	MWH_UniP1_aquatic_group	1159:1181	Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera.					
31216303	4	66	theme	extracellular	860:872	arg1	polysaccharides					874:888	extracellular polysaccharides	860:888	extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components	860:964	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	4	66	theme	extracellular	860:872	arg1	EPS					891:893	EPS	891:893	EPS	891:893	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	15	67	from	strains	1644:1650	arg1	EPS					1607:1609	EPS	1607:1609	EPS from the bioflocculant-producing strains	1607:1650	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	0	68	from	farm	163:166	arg1	resource					88:95	a promising natural bioflocculant resource	54:95	a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	54:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	0	68	from	farm	163:166	arg1	China					181:185	China	181:185	China	181:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	12	69	theme	Bacillus	1465:1472	arg1	sp					1474:1475	Bacillus sp	1465:1475	Bacillus sp	1465:1475	(1) and Bacillus sp.					
31216303	17	70	theme	Zhoushan	1878:1885	arg1	RPM					1887:1889	Zhoushan RPM	1878:1889	Zhoushan RPM	1878:1889	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	15	71	theme	monosaccharide	1677:1690	arg1	composition					1692:1702	highly similar monosaccharide composition	1662:1702	highly similar monosaccharide composition	1662:1702	Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides.					
31216303	6	72	theme	bioflocculant-producing	1248:1270	arg1	strains					1272:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	6	72	theme	bioflocculant-producing	1248:1270	arg1	sp					1347:1348	Pseudoalteromonas sp	1329:1348	Pseudoalteromonas sp	1329:1348	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	13	73	theme	Bacillus	1503:1510	arg1	sp					1512:1513	Bacillus sp	1503:1513	Bacillus sp	1503:1513	(1), all of which except Bacillus sp.					
31216303	4	74	theme	RPM	654:656	arg1	samples					658:664	RPM samples	654:664	RPM samples from an aquaculture farm in Zhoushan of China	654:710	RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components.					
31216303	0	75	theme	bioflocculant	74:86	arg1	resource					88:95	a promising natural bioflocculant resource	54:95	a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	54:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31216303	6	76	theme	efficient	1238:1246	arg1	strains					1272:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains	1222:1278	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	6	76	theme	efficient	1238:1246	arg1	sp					1347:1348	Pseudoalteromonas sp	1329:1348	Pseudoalteromonas sp	1329:1348	Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp.					
31216303	17	77	theme	fermentation	2006:2017	arg1	approach					2019:2026	fermentation approach	2006:2026	fermentation approach	2006:2026	Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.					
31216303	16	78	theme	fungi	1800:1804	arg1	existence					1787:1795	the existence	1783:1795	the existence of fungi in RPM	1783:1811	On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality.					
31216303	16	78	theme	fungi	1800:1804	arg1	rare					1817:1820	rare	1817:1820	rare	1817:1820	On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality.					
31216303	0	79	theme	natural	66:72	arg1	resource					88:95	a promising natural bioflocculant resource	54:95	a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China	54:185	Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.					
31320092	4	0	theme	surface	572:578	arg1	analysis					590:597	the morphological and surface roughness analysis	550:597	analysis	590:597	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	5	1	theme	Fourier-transform	756:772	arg1	spectroscopy					790:801	Fourier-transform infrared (FTIR) spectroscopy	756:801	Fourier-transform infrared (FTIR) spectroscopy	756:801	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	2	2	theme	physicochemical	322:336	arg1	characteristics					338:352	physicochemical characteristics	322:352	physicochemical characteristics	322:352	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	5	3	theme	scanning	924:931	arg1	calorimetry					933:943	differential scanning calorimetry	911:943	differential scanning calorimetry (DSC)	911:949	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	3	theme	scanning	924:931	arg1	DSC					946:948	DSC	946:948	DSC	946:948	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	3	4	dep	damaging	514:521	arg1	them					523:526	them	523:526	them	523:526	The difficulties encountered are associated to the extraction of the fibers without damaging them.					
31320092	5	5	theme	thermal	713:719	arg1	properties					721:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	2	6	dep	morphology	284:293	arg1	the					280:282	the	280:282	the	280:282	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	4	7	theme	electron	626:633	arg1	microscopies					635:646	scanning electron microscopies	617:646	scanning electron microscopies	617:646	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	2	8	theme	study	256:260	arg1	aim					244:246	The aim	240:246	The aim of this study	240:260	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	5	9	theme	X-ray	848:852	arg1	XRD					867:869	XRD	867:869	XRD	867:869	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	9	theme	X-ray	848:852	arg1	diffraction					854:864	X-ray diffraction	848:864	X-ray diffraction (XRD)	848:870	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	2	10	theme	fiber	383:387	arg1	strands					389:395	fiber strands	383:395	fiber strands	383:395	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	0	11	theme	potential	93:101	arg1	bio-reinforcement					103:119	potential bio-reinforcement	93:119	potential bio-reinforcement in composite	93:132	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	5	12	theme	infrared	774:781	arg1	spectroscopy					790:801	Fourier-transform infrared (FTIR) spectroscopy	756:801	Fourier-transform infrared (FTIR) spectroscopy	756:801	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	6	13	theme	Weibull	1052:1058	arg1	distribution					1060:1071	two-parameter Weibull distribution	1038:1071	two-parameter Weibull distribution	1038:1071	The mechanical properties were accessed by tensile tests and they were analyzed using two-parameter Weibull distribution.					
31320092	4	14	theme	roughness	580:588	arg1	analysis					590:597	the morphological and surface roughness analysis	550:597	analysis	590:597	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	5	15	dep	infrared	774:781	arg1	FTIR					784:787	FTIR	784:787	FTIR	784:787	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	2	16	theme	bundles	371:377	arg1	characteristics					338:352	physicochemical characteristics	322:352	physicochemical characteristics	322:352	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	2	16	theme	bundles	371:377	arg1	structure					296:304	structure	296:304	structure	296:304	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	2	16	theme	bundles	371:377	arg1	morphology					284:293	morphology	284:293	morphology	284:293	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	6	17	theme	mechanical	956:965	arg1	properties					967:976	The mechanical properties	952:976	The mechanical properties	952:976	The mechanical properties were accessed by tensile tests and they were analyzed using two-parameter Weibull distribution.					
31320092	2	18	theme	vascular	362:369	arg1	bundles					371:377	vascular bundles	362:377	vascular bundles	362:377	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	5	19	theme	energy	804:809	arg1	EDX					842:844	EDX	842:844	EDX	842:844	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	19	theme	energy	804:809	arg1	spectroscopy					828:839	energy dispersive X-ray spectroscopy	804:839	energy dispersive X-ray spectroscopy (EDX)	804:845	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	20	theme	differential	911:922	arg1	calorimetry					933:943	differential scanning calorimetry	911:943	differential scanning calorimetry (DSC)	911:949	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	20	theme	differential	911:922	arg1	DSC					946:948	DSC	946:948	DSC	946:948	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	2	21	theme	date	412:415	arg1	rachis					422:427	date palm rachis	412:427	date palm rachis	412:427	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	2	22	theme	palm	417:420	arg1	rachis					422:427	date palm rachis	412:427	date palm rachis	412:427	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	5	23	theme	chemical	690:697	arg1	properties					721:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	3	24	theme	fibers	499:504	arg1	extraction					481:490	the extraction	477:490	the extraction of the fibers without damaging them	477:526	The difficulties encountered are associated to the extraction of the fibers without damaging them.					
31320092	4	25	theme	3D	673:674	arg1	profiler					676:683	a non-contact 3D profiler	659:683	a non-contact 3D profiler	659:683	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	0	26	theme	bundle	44:49	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	0	26	theme	bundle	44:49	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	0	27	from	date	73:76	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	0	27	from	date	73:76	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	0	28	theme	vascular	35:42	arg1	bundle					44:49	vascular bundle	35:49	vascular bundle	35:49	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	5	29	theme	physical	700:707	arg1	properties					721:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties	686:730	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	6	30	theme	two-parameter	1038:1050	arg1	distribution					1060:1071	two-parameter Weibull distribution	1038:1071	two-parameter Weibull distribution	1038:1071	The mechanical properties were accessed by tensile tests and they were analyzed using two-parameter Weibull distribution.					
31320092	2	31	theme	strands	389:395	arg1	characteristics					338:352	physicochemical characteristics	322:352	physicochemical characteristics	322:352	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	2	31	theme	strands	389:395	arg1	structure					296:304	structure	296:304	structure	296:304	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	2	31	theme	strands	389:395	arg1	morphology					284:293	morphology	284:293	morphology	284:293	The aim of this study is to investigate the morphology, structure, mechanical and physicochemical characteristics of both vascular bundles and fiber strands extracted from date palm rachis.					
31320092	0	32	theme	fiber	55:59	arg1	strand					61:66	fiber strand	55:66	fiber strand	55:66	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	1	33	dep	rich	163:166	arg1	inexpensive					193:203	inexpensive	193:203	inexpensive	193:203	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	5	34	theme	thermogravimetric	873:889	arg1	analysis					891:898	thermogravimetric analysis	873:898	thermogravimetric analysis (TGA)	873:904	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	34	theme	thermogravimetric	873:889	arg1	TGA					901:903	TGA	901:903	TGA	901:903	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	1	35	from	Algeria	231:237	arg1	available					218:226	available	218:226	available	218:226	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	0	36	from	bio-reinforcement	103:119	arg1	composite					124:132	composite	124:132	composite	124:132	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	4	37	theme	non-contact	661:671	arg1	profiler					676:683	a non-contact 3D profiler	659:683	a non-contact 3D profiler	659:683	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	1	38	from	available	218:226	arg1	Algeria					231:237	Algeria	231:237	Algeria	231:237	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	1	39	from	cellulose	171:179	arg1	rich					163:166	rich	163:166	rich	163:166	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	1	40	theme	Date	135:138	arg1	fibers					152:157	Date palm rachis fibers	135:157	Date palm rachis fibers	135:157	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	6	41	theme	tensile	995:1001	arg1	tests					1003:1007	tensile tests	995:1007	tensile tests	995:1007	The mechanical properties were accessed by tensile tests and they were analyzed using two-parameter Weibull distribution.					
31320092	5	42	theme	X-ray	822:826	arg1	EDX					842:844	EDX	842:844	EDX	842:844	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	42	theme	X-ray	822:826	arg1	spectroscopy					828:839	energy dispersive X-ray spectroscopy	804:839	energy dispersive X-ray spectroscopy (EDX)	804:845	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	1	43	theme	palm	140:143	arg1	fibers					152:157	Date palm rachis fibers	135:157	Date palm rachis fibers	135:157	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	4	44	theme	scanning	617:624	arg1	microscopies					635:646	scanning electron microscopies	617:646	scanning electron microscopies	617:646	The study focuses on the morphological and surface roughness analysis using optical and scanning electron microscopies (SEM), and a non-contact 3D profiler.					
31320092	0	45	theme	strand	61:66	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	0	45	theme	strand	61:66	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of vascular bundle and fiber strand from date palm rachis as potential bio-reinforcement in composite.					
31320092	5	46	theme	dispersive	811:820	arg1	EDX					842:844	EDX	842:844	EDX	842:844	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	5	46	theme	dispersive	811:820	arg1	spectroscopy					828:839	energy dispersive X-ray spectroscopy	804:839	energy dispersive X-ray spectroscopy (EDX)	804:845	The chemical, physical and thermal properties have been studied using Fourier-transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC).					
31320092	1	47	from	rich	163:166	arg1	cellulose					171:179	cellulose	171:179	cellulose	171:179	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31320092	1	48	theme	rachis	145:150	arg1	fibers					152:157	Date palm rachis fibers	135:157	Date palm rachis fibers	135:157	Date palm rachis fibers are rich in cellulose, relatively inexpensive, and readily available in Algeria.					
31489667	6	0	dep	activity	1375:1382	arg1	test					1384:1387	test	1384:1387	test	1384:1387	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	10	1	used	used	2104:2107	arg2	sources					2130:2136	bioactive natural sources	2112:2136	bioactive natural sources in the pharmaceutical, food, and cosmetic industries	2112:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	10	1	used	used	2104:2107	arg2	polysaccharides					2079:2093	these polysaccharides	2073:2093	these polysaccharides	2073:2093	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	9	2	theme	chemical	1794:1801	arg1	composition					1803:1813	the chemical composition	1790:1813	the chemical composition	1790:1813	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	7	3	theme	bioactive	1564:1572	arg1	compounds					1574:1582	bioactive compounds	1564:1582	bioactive compounds	1564:1582	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	9	4	theme	structural	1816:1825	arg1	characterization					1827:1842	structural characterization	1816:1842	structural characterization	1816:1842	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	4	5	theme	array	949:953	arg1	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	5	6	theme	DPPH•	1304:1308	arg1	assays					1338:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	7	7	theme	various	1589:1595	arg1	activities					1608:1617	various biological activities	1589:1617	various biological activities	1589:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	4	8	theme	molecular	991:999	arg1	weight					1001:1006	the molecular weight	987:1006	the molecular weight of the polysaccharides	987:1029	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	8	9	theme	Several	1620:1626	arg1	studies					1628:1634	Several studies	1620:1634	Several studies	1620:1634	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	3	10	theme	chromatography-mass	796:814	arg1	spectrometry					816:827	gas chromatography-mass spectrometry	792:827	gas chromatography-mass spectrometry	792:827	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	1	11	theme	pini	391:394	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	11	theme	pini	391:394	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	10	12	from	sources	2130:2136	arg1	industries					2180:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	5	13	dep	displayed	1096:1104	arg1	whereas					1140:1146	whereas	1140:1146	whereas	1140:1146	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	6	14	theme	significant	1416:1426	arg1	activity					1439:1446	significant inhibitory activity	1416:1446	significant inhibitory activity against butyrylcholinesterase enzyme	1416:1483	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	5	15	theme	cupric	1198:1203	arg1	capacity					1226:1233	cupric reducing antioxidant capacity	1198:1233	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml)	1198:1261	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	10	16	theme	polysaccharide	1985:1998	arg1	extracts					2000:2007	polysaccharide extracts	1985:2007	polysaccharide extracts obtained from tree mushrooms	1985:2036	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	9	17	from	composition	1803:1813	arg1	mushrooms					1934:1942	studied mushrooms	1926:1942	studied mushrooms in detail	1926:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	2	18	theme	protein	618:624	arg1	contents					626:633	total protein contents	612:633	total protein contents	612:633	Total carbohydrate contents of the polysaccharides were ranged between 65.06 ± 6.76 and 88.27 ± 5.15 µg/mg and total protein contents were ranged between 3.18 ± 0.72 and 6.56 ± 1.25 µg/mg.					
31489667	10	19	theme	cosmetic	2171:2178	arg1	industries					2180:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	1	20	theme	tree	401:404	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	20	theme	tree	401:404	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	7	21	from	mushrooms	1531:1539	arg1	Polysaccharides					1510:1524	Polysaccharides	1510:1524	Polysaccharides from mushrooms	1510:1539	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	21	from	mushrooms	1531:1539	arg1	APPLICATIONS					1496:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	21	from	mushrooms	1531:1539	arg1	class					1555:1559	the major class	1545:1559	the major class of bioactive compounds with various biological activities	1545:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	9	22	theme	extracted	1895:1903	arg1	polysaccharides					1905:1919	extracted polysaccharides	1895:1919	extracted polysaccharides from studied mushrooms in detail	1895:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	5	23	dep	IC50	1070:1073	arg1	2.55 ± 0.40 µg/ml					1077:1093	2.55 ± 0.40 µg/ml	1077:1093	IC50 : 2.55 ± 0.40 µg/ml	1070:1093	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	24	theme	highest	1166:1172	arg1	activity					1186:1193	the highest antioxidant activity	1162:1193	the highest antioxidant activity	1162:1193	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	10	25	theme	tree	2023:2026	arg1	mushrooms					2028:2036	tree mushrooms	2023:2036	tree mushrooms	2023:2036	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	5	26	theme	β-carotene-linoleic	1035:1053	arg1	FF					1066:1067	β-carotene-linoleic acid assay FF	1035:1067	β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml)	1035:1094	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	26	theme	β-carotene-linoleic	1035:1053	arg1	IC50					1070:1073	IC50	1070:1073	IC50 : 2.55 ± 0.40 µg/ml	1070:1093	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	0	27	theme	different	75:83	arg1	polysaccharides					85:99	different polysaccharides	75:99	different polysaccharides	75:99	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	1	28	theme	Pleurotus	348:356	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	28	theme	Pleurotus	348:356	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	2	29	theme	Total	501:505	arg1	contents					520:527	Total carbohydrate contents	501:527	Total carbohydrate contents of the polysaccharides	501:550	Total carbohydrate contents of the polysaccharides were ranged between 65.06 ± 6.76 and 88.27 ± 5.15 µg/mg and total protein contents were ranged between 3.18 ± 0.72 and 6.56 ± 1.25 µg/mg.					
31489667	1	30	theme	Ganoderma	308:316	arg1	lucidum					318:324	Ganoderma lucidum	308:324	Ganoderma lucidum	308:324	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	0	31	theme	tree	116:119	arg1	species					130:136	tree mushroom species	116:136	tree mushroom species	116:136	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	5	32	theme	antioxidant	1214:1224	arg1	capacity					1226:1233	cupric reducing antioxidant capacity	1198:1233	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml)	1198:1261	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	1	33	theme	structural	164:173	arg1	characterization					175:190	structural characterization	164:190	structural characterization	164:190	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	4	34	theme	characteristic	856:869	arg1	peaks					871:875	the characteristic peaks	852:875	the characteristic peaks of the polysaccharides	852:898	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	6	35	theme	butyrylcholinesterase	1456:1476	arg1	enzyme					1478:1483	butyrylcholinesterase enzyme	1456:1483	butyrylcholinesterase enzyme	1456:1483	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	0	36	theme	biological	49:58	arg1	activities					60:69	biological activities	49:69	biological activities	49:69	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	0	37	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	1	38	theme	antioxidant	421:431	arg1	activities					456:465	antioxidant and anticholinesterase activities	421:465	activities	456:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	5	39	theme	assay	1060:1064	arg1	FF					1066:1067	β-carotene-linoleic acid assay FF	1035:1067	β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml)	1035:1094	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	39	theme	assay	1060:1064	arg1	IC50					1070:1073	IC50	1070:1073	IC50 : 2.55 ± 0.40 µg/ml	1070:1093	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	8	40	theme	extracts	1700:1707	arg1	activity					1669:1676	the biological activity	1654:1676	the biological activity of the polysaccharide extracts from different mushrooms	1654:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	4	41	theme	FT-IR	830:834	arg1	analysis					836:843	FT-IR analysis	830:843	FT-IR analysis	830:843	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	4	42	used	used	969:972	arg2	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	8	43	theme	different	1714:1722	arg1	mushrooms					1724:1732	different mushrooms	1714:1732	different mushrooms	1714:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	10	44	theme	significant	2045:2055	arg1	bioactivity					2057:2067	a significant bioactivity	2043:2067	a significant bioactivity	2043:2067	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	4	45	theme	high	904:907	arg1	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	5	46	theme	ABTS•+	1264:1269	arg1	assays					1338:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	47	dep	A0.50	1236:1240	arg1	59.90 ± 0.53 μg/ml					1243:1260	59.90 ± 0.53 μg/ml	1243:1260	A0.50 :59.90 ± 0.53 μg/ml	1236:1260	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	9	48	theme	studied	1926:1932	arg1	mushrooms					1934:1942	studied mushrooms	1926:1942	studied mushrooms in detail	1926:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	4	49	theme	liquid	921:926	arg1	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	8	50	from	activity	1669:1676	arg1	mushrooms					1724:1732	different mushrooms	1714:1732	different mushrooms	1714:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	1	51	theme	igniarius	337:345	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	51	theme	igniarius	337:345	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	9	52	from	mushrooms	1934:1942	arg1	detail					1947:1952	detail	1947:1952	detail	1947:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	52	from	mushrooms	1934:1942	arg1	polysaccharides					1905:1919	extracted polysaccharides	1895:1919	extracted polysaccharides from studied mushrooms in detail	1895:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	52	from	mushrooms	1934:1942	arg1	antioxidant					1845:1855	antioxidant	1845:1855	antioxidant	1845:1855	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	52	from	mushrooms	1934:1942	arg1	composition					1803:1813	the chemical composition	1790:1813	the chemical composition	1790:1813	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	52	from	mushrooms	1934:1942	arg1	characterization					1827:1842	structural characterization	1816:1842	structural characterization	1816:1842	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	52	from	mushrooms	1934:1942	arg1	activities					1881:1890	anticholinesterase activities	1862:1890	anticholinesterase activities	1862:1890	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	6	53	theme	inhibitory	1364:1373	arg1	activity					1375:1382	cholinesterase inhibitory activity test	1349:1387	cholinesterase inhibitory activity test	1349:1387	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	5	54	dep	capacity	1226:1233	arg1	A0.50					1236:1240	A0.50	1236:1240	A0.50 :59.90 ± 0.53 μg/ml	1236:1260	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	4	55	theme	polysaccharides	884:898	arg1	peaks					871:875	the characteristic peaks	852:875	the characteristic peaks of the polysaccharides	852:898	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	5	56	theme	antioxidant	1118:1128	arg1	activity					1130:1137	the highest antioxidant activity	1106:1137	the highest antioxidant activity	1106:1137	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	8	57	from	mushrooms	1724:1732	arg1	extracts					1700:1707	the polysaccharide extracts	1681:1707	the polysaccharide extracts from different mushrooms	1681:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	8	57	from	mushrooms	1724:1732	arg1	activity					1669:1676	the biological activity	1654:1676	the biological activity of the polysaccharide extracts from different mushrooms	1654:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	1	58	theme	Ganoderma	280:288	arg1	applanatum					290:299	Ganoderma applanatum	280:299	Ganoderma applanatum (GAP)	280:305	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	58	theme	Ganoderma	280:288	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	58	theme	Ganoderma	280:288	arg1	GAP					302:304	GAP	302:304	GAP	302:304	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	3	59	theme	major	741:745	arg1	monosaccharides					747:761	major monosaccharides	741:761	major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry	741:827	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	3	59	theme	major	741:745	arg1	glucose					701:707	glucose	701:707	glucose	701:707	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	3	59	theme	major	741:745	arg1	Galactose					690:698	Galactose	690:698	Galactose	690:698	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	3	59	theme	major	741:745	arg1	mannose					714:720	mannose	714:720	mannose	714:720	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	1	60	theme	anticholinesterase	437:454	arg1	activities					456:465	antioxidant and anticholinesterase activities	421:465	activities	456:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	7	61	theme	biological	1597:1606	arg1	activities					1608:1617	various biological activities	1589:1617	various biological activities	1589:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	62	theme	compounds	1574:1582	arg1	Polysaccharides					1510:1524	Polysaccharides	1510:1524	Polysaccharides from mushrooms	1510:1539	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	62	theme	compounds	1574:1582	arg1	APPLICATIONS					1496:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	62	theme	compounds	1574:1582	arg1	class					1555:1559	the major class	1545:1559	the major class of bioactive compounds with various biological activities	1545:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	3	63	theme	gas	792:794	arg1	spectrometry					816:827	gas chromatography-mass spectrometry	792:827	gas chromatography-mass spectrometry	792:827	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	1	64	theme	Fomes	214:218	arg1	FF					233:234	FF	233:234	FF	233:234	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	64	theme	Fomes	214:218	arg1	adspersum					269:277	Ganoderma adspersum	259:277	Ganoderma adspersum	259:277	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	64	theme	Fomes	214:218	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	64	theme	Fomes	214:218	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	64	theme	Fomes	214:218	arg1	torulosa					249:256	torulosa	249:256	torulosa	249:256	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	64	theme	Fomes	214:218	arg1	applanatum					290:299	Ganoderma applanatum	280:299	Ganoderma applanatum (GAP)	280:305	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	0	65	theme	activities	60:69	arg1	determination					32:44	determination	32:44	determination	32:44	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	0	65	theme	activities	60:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	4	66	theme	chromatography-diode	928:947	arg1	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	10	67	theme	natural	2122:2128	arg1	sources					2130:2136	bioactive natural sources	2112:2136	bioactive natural sources in the pharmaceutical, food, and cosmetic industries	2112:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	10	67	theme	natural	2122:2128	arg1	polysaccharides					2079:2093	these polysaccharides	2073:2093	these polysaccharides	2073:2093	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	6	68	theme	cholinesterase	1349:1362	arg1	activity					1375:1382	cholinesterase inhibitory activity test	1349:1387	cholinesterase inhibitory activity test	1349:1387	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	9	69	from	characterization	1827:1842	arg1	mushrooms					1934:1942	studied mushrooms	1926:1942	studied mushrooms in detail	1926:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	7	70	theme	major	1549:1553	arg1	Polysaccharides					1510:1524	Polysaccharides	1510:1524	Polysaccharides from mushrooms	1510:1539	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	70	theme	major	1549:1553	arg1	APPLICATIONS					1496:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	70	theme	major	1549:1553	arg1	class					1555:1559	the major class	1545:1559	the major class of bioactive compounds with various biological activities	1545:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	10	71	theme	food	2161:2164	arg1	industries					2180:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	4	72	theme	polysaccharides	1015:1029	arg1	weight					1001:1006	the molecular weight	987:1006	the molecular weight of the polysaccharides	987:1029	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	10	73	theme	pharmaceutical	2145:2158	arg1	industries					2180:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	the pharmaceutical, food, and cosmetic industries	2141:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	6	74	theme	inhibitory	1428:1437	arg1	activity					1439:1446	significant inhibitory activity	1416:1446	significant inhibitory activity against butyrylcholinesterase enzyme	1416:1483	In cholinesterase inhibitory activity test, PO (56.31±0.0.74%) showed significant inhibitory activity against butyrylcholinesterase enzyme.					
31489667	9	75	theme	anticholinesterase	1862:1879	arg1	activities					1881:1890	anticholinesterase activities	1862:1890	anticholinesterase activities	1862:1890	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	3	76	from	monosaccharides	747:761	arg1	polysaccharides					770:784	all polysaccharides	766:784	all polysaccharides using gas chromatography-mass spectrometry	766:827	Galactose, glucose, and mannose were identified as major monosaccharides in all polysaccharides using gas chromatography-mass spectrometry.					
31489667	5	77	theme	reducing	1205:1212	arg1	capacity					1226:1233	cupric reducing antioxidant capacity	1198:1233	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml)	1198:1261	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	1	78	theme	Chemical	139:146	arg1	composition					148:158	Chemical composition	139:158	Chemical composition	139:158	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	2	79	theme	total	612:616	arg1	contents					626:633	total protein contents	612:633	total protein contents	612:633	Total carbohydrate contents of the polysaccharides were ranged between 65.06 ± 6.76 and 88.27 ± 5.15 µg/mg and total protein contents were ranged between 3.18 ± 0.72 and 6.56 ± 1.25 µg/mg.					
31489667	5	80	dep	IC50	1311:1314	arg1	45.58 ± 0.21 μg/ml					1318:1335	45.58 ± 0.21 μg/ml	1318:1335	IC50 : 45.58 ± 0.21 μg/ml	1311:1335	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	9	81	theme	polysaccharides	1905:1919	arg1	antioxidant					1845:1855	antioxidant	1845:1855	antioxidant	1845:1855	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	81	theme	polysaccharides	1905:1919	arg1	composition					1803:1813	the chemical composition	1790:1813	the chemical composition	1790:1813	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	81	theme	polysaccharides	1905:1919	arg1	characterization					1827:1842	structural characterization	1816:1842	structural characterization	1816:1842	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	81	theme	polysaccharides	1905:1919	arg1	activities					1881:1890	anticholinesterase activities	1862:1890	anticholinesterase activities	1862:1890	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	7	82	theme	PRACTICAL	1486:1494	arg1	Polysaccharides					1510:1524	Polysaccharides	1510:1524	Polysaccharides from mushrooms	1510:1539	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	82	theme	PRACTICAL	1486:1494	arg1	class					1555:1559	the major class	1545:1559	the major class of bioactive compounds with various biological activities	1545:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	7	82	theme	PRACTICAL	1486:1494	arg1	APPLICATIONS					1496:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS	1486:1507	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	5	83	theme	antioxidant	1174:1184	arg1	activity					1186:1193	the highest antioxidant activity	1162:1193	the highest antioxidant activity	1162:1193	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	9	84	from	activities	1881:1890	arg1	mushrooms					1934:1942	studied mushrooms	1926:1942	studied mushrooms in detail	1926:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	2	85	theme	carbohydrate	507:518	arg1	contents					520:527	Total carbohydrate contents	501:527	Total carbohydrate contents of the polysaccharides	501:550	Total carbohydrate contents of the polysaccharides were ranged between 65.06 ± 6.76 and 88.27 ± 5.15 µg/mg and total protein contents were ranged between 3.18 ± 0.72 and 6.56 ± 1.25 µg/mg.					
31489667	1	86	theme	polysaccharides	195:209	arg1	composition					148:158	Chemical composition	139:158	Chemical composition	139:158	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	86	theme	polysaccharides	195:209	arg1	characterization					175:190	structural characterization	164:190	structural characterization	164:190	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	8	87	theme	biological	1658:1667	arg1	activity					1669:1676	the biological activity	1654:1676	the biological activity of the polysaccharide extracts from different mushrooms	1654:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	0	88	theme	mushroom	121:128	arg1	species					130:136	tree mushroom species	116:136	tree mushroom species	116:136	Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species.					
31489667	9	89	from	antioxidant	1845:1855	arg1	mushrooms					1934:1942	studied mushrooms	1926:1942	studied mushrooms in detail	1926:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	2	90	theme	polysaccharides	536:550	arg1	contents					520:527	Total carbohydrate contents	501:527	Total carbohydrate contents of the polysaccharides	501:550	Total carbohydrate contents of the polysaccharides were ranged between 65.06 ± 6.76 and 88.27 ± 5.15 µg/mg and total protein contents were ranged between 3.18 ± 0.72 and 6.56 ± 1.25 µg/mg.					
31489667	5	91	dep	IC50	1272:1275	arg1	16.62 ± 0.31 μg/ml					1279:1296	16.62 ± 0.31 μg/ml	1279:1296	IC50 : 16.62 ± 0.31 μg/ml	1272:1296	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	92	theme	capacity	1226:1233	arg1	assays					1338:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays	1198:1343	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	9	93	from	report	1780:1785	arg1	antioxidant					1845:1855	antioxidant	1845:1855	antioxidant	1845:1855	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	93	from	report	1780:1785	arg1	composition					1803:1813	the chemical composition	1790:1813	the chemical composition	1790:1813	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	93	from	report	1780:1785	arg1	characterization					1827:1842	structural characterization	1816:1842	structural characterization	1816:1842	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	93	from	report	1780:1785	arg1	activities					1881:1890	anticholinesterase activities	1862:1890	anticholinesterase activities	1862:1890	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	8	94	theme	polysaccharide	1685:1698	arg1	extracts					1700:1707	the polysaccharide extracts	1681:1707	the polysaccharide extracts from different mushrooms	1681:1732	Several studies were performed on the biological activity of the polysaccharide extracts from different mushrooms.					
31489667	1	95	theme	Porodaedalea	378:389	arg1	PP					397:398	PP	397:398	PP	397:398	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	95	theme	Porodaedalea	378:389	arg1	pini					391:394	Porodaedalea pini	378:394	Porodaedalea pini (PP)	378:399	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	96	dep	Fomes	214:218	arg1	fomentarius					220:230	Fomes fomentarius	214:230	Fomes fomentarius	214:230	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	5	97	theme	acid	1055:1058	arg1	FF					1066:1067	β-carotene-linoleic acid assay FF	1035:1067	β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml)	1035:1094	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	5	97	theme	acid	1055:1058	arg1	IC50					1070:1073	IC50	1070:1073	IC50 : 2.55 ± 0.40 µg/ml	1070:1093	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	1	98	with	mushrooms	406:414	arg1	activities					456:465	antioxidant and anticholinesterase activities	421:465	activities	456:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	9	99	theme	first	1774:1778	arg1	this					1762:1765	this	1762:1765	this	1762:1765	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	9	99	theme	first	1774:1778	arg1	report					1780:1785	the first report	1770:1785	the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail	1770:1952	However, to our knowledge, this is the first report on the chemical composition, structural characterization, antioxidant, and anticholinesterase activities of extracted polysaccharides from studied mushrooms in detail.					
31489667	1	100	dep	Pleurotus	348:356	arg1	ostreatus					358:366	Pleurotus ostreatus (PO)	348:371	Pleurotus ostreatus (PO)	348:371	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	7	101	with	class	1555:1559	arg1	activities					1608:1617	various biological activities	1589:1617	various biological activities	1589:1617	PRACTICAL APPLICATIONS: Polysaccharides from mushrooms are the major class of bioactive compounds with various biological activities.					
31489667	1	102	theme	lucidum	318:324	arg1	mushrooms					406:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms	308:414	Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities	308:465	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	102	theme	lucidum	318:324	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	103	theme	Phellinus	327:335	arg1	igniarius					337:345	Phellinus igniarius	327:345	Phellinus igniarius	327:345	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	4	104	theme	performance	909:919	arg1	detection					955:963	high performance liquid chromatography-diode array detection	904:963	high performance liquid chromatography-diode array detection	904:963	FT-IR analysis showed the characteristic peaks of the polysaccharides and high performance liquid chromatography-diode array detection was used to determine the molecular weight of the polysaccharides.					
31489667	1	105	theme	Ganoderma	259:267	arg1	adspersum					269:277	Ganoderma adspersum	259:277	Ganoderma adspersum	259:277	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	1	105	theme	Ganoderma	259:267	arg1	polysaccharides					195:209	polysaccharides	195:209	polysaccharides of Fomes fomentarius (FF)	195:235	Chemical composition and structural characterization of polysaccharides of Fomes fomentarius (FF), Fuscoporia torulosa, Ganoderma adspersum, Ganoderma applanatum (GAP), Ganoderma lucidum, Phellinus igniarius, Pleurotus ostreatus (PO), and Porodaedalea pini (PP) tree mushrooms with antioxidant and anticholinesterase activities were determined in this research.					
31489667	5	106	theme	highest	1110:1116	arg1	activity					1130:1137	the highest antioxidant activity	1106:1137	the highest antioxidant activity	1106:1137	In β-carotene-linoleic acid assay FF (IC50 : 2.55 ± 0.40 µg/ml) displayed the highest antioxidant activity, whereas GAP indicated the highest antioxidant activity in cupric reducing antioxidant capacity (A0.50 :59.90 ± 0.53 μg/ml), ABTS•+ (IC50 : 16.62 ± 0.31 μg/ml), and DPPH• (IC50 : 45.58 ± 0.21 μg/ml) assays.					
31489667	10	107	theme	bioactive	2112:2120	arg1	sources					2130:2136	bioactive natural sources	2112:2136	bioactive natural sources in the pharmaceutical, food, and cosmetic industries	2112:2189	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
31489667	10	107	theme	bioactive	2112:2120	arg1	polysaccharides					2079:2093	these polysaccharides	2073:2093	these polysaccharides	2073:2093	This investigation shows that polysaccharide extracts obtained from tree mushrooms show a significant bioactivity and these polysaccharides might be used as bioactive natural sources in the pharmaceutical, food, and cosmetic industries.					
30545217	7	0	theme	RNA-seq	979:985	arg1	analysis					987:994	RNA-seq analysis	979:994	RNA-seq analysis	979:994	This was further affirmed by RNA-seq analysis, where a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated) were identified between yft1 and cv.					
30545217	9	1	theme	auxin-related	1191:1203	arg1	transduction					1212:1223	ethylene- and auxin-related signal transduction	1177:1223	ethylene- and auxin-related signal transduction	1177:1223	These DEGs were mainly annotated as participating in ethylene- and auxin-related signal transduction, sugar metabolism, and photosynthesis.					
30545217	0	2	theme	Transcriptional	54:68	arg1	Profile					70:76	Transcriptional Profile	54:76	Transcriptional Profile	54:76	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	1	3	theme	fruit	285:289	arg1	softening					291:299	fruit softening	285:299	fruit softening	285:299	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	10	4	theme	new	1284:1286	arg1	insights					1288:1295	new insights	1284:1295	new insights into the mechanism underlying the control of fruit softening	1284:1356	This study provides new insights into the mechanism underlying the control of fruit softening.					
30545217	1	5	theme	Fruit	123:127	arg1	firmness					129:136	Fruit firmness	123:136	Fruit firmness	123:136	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	1	5	theme	Fruit	123:127	arg1	trait					154:158	an important trait	141:158	an important trait	141:158	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	9	6	theme	sugar	1226:1230	arg1	metabolism					1232:1241	sugar metabolism	1226:1241	sugar metabolism	1226:1241	These DEGs were mainly annotated as participating in ethylene- and auxin-related signal transduction, sugar metabolism, and photosynthesis.					
30545217	0	7	from	Changes	0:6	arg1	Mutant					115:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	0	7	from	Changes	0:6	arg1	Firmness					17:24	Fruit Firmness	11:24	Fruit Firmness	11:24	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	0	7	from	Changes	0:6	arg1	Profile					70:76	Transcriptional Profile	54:76	Transcriptional Profile	54:76	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	0	7	from	Changes	0:6	arg1	Composition					37:47	Cell Wall Composition	27:47	Cell Wall Composition	27:47	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	1	8	theme	shelf	211:215	arg1	life					217:220	shelf life	211:220	shelf life	211:220	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	4	9	from	difference	624:633	arg1	wall					706:709	the primary cell wall	689:709	the primary cell wall (PCW)	689:715	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	9	from	difference	624:633	arg1	deposition					675:684	pectin deposition	668:684	pectin deposition	668:684	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	9	from	difference	624:633	arg1	hemicellulose					649:661	hemicellulose	649:661	hemicellulose	649:661	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	9	from	difference	624:633	arg1	PCW					712:714	PCW	712:714	PCW	712:714	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	9	from	difference	624:633	arg1	cellulose					638:646	cellulose	638:646	cellulose	638:646	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	3	10	theme	M82	456:458	arg1	type					465:468	M82 wild type	456:468	M82 wild type	456:468	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	6	11	theme	hydrolase	811:819	arg1	activities					821:830	hydrolase activities	811:830	hydrolase activities	811:830	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	0	12	theme	fruit	92:96	arg1	Mutant					115:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	4	13	theme	cell	701:704	arg1	wall					706:709	the primary cell wall	689:709	the primary cell wall (PCW)	689:715	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	13	theme	cell	701:704	arg1	PCW					712:714	PCW	712:714	PCW	712:714	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	0	14	theme	yellow	85:90	arg1	Mutant					115:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	4	15	theme	primary	693:699	arg1	wall					706:709	the primary cell wall	689:709	the primary cell wall (PCW)	689:715	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	15	theme	primary	693:699	arg1	PCW					712:714	PCW	712:714	PCW	712:714	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	6	16	theme	genes	851:855	arg1	components					799:808	chemical components	790:808	chemical components	790:808	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	16	theme	genes	851:855	arg1	activities					821:830	hydrolase activities	811:830	hydrolase activities	811:830	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	16	theme	genes	851:855	arg1	expression					837:846	expression	837:846	expression of genes encoding these hydrolases	837:881	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	16	theme	genes	851:855	arg1	structure					757:765	the structure	753:765	the structure of the pericarp cells	753:787	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	2	17	theme	tomato	333:338	arg1	mutant					350:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	10	18	theme	softening	1348:1356	arg1	control					1331:1337	the control	1327:1337	the control of fruit softening	1327:1356	This study provides new insights into the mechanism underlying the control of fruit softening.					
30545217	7	19	theme	expressed	1033:1041	arg1	genes					1043:1047	183 differentially expressed genes	1014:1047	183 differentially expressed genes (DEGs, 50/133 down-/upregulated)	1014:1080	This was further affirmed by RNA-seq analysis, where a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated) were identified between yft1 and cv.					
30545217	2	20	theme	fruit	327:331	arg1	mutant					350:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	2	21	theme	genetic	367:373	arg1	lesion					375:380	a genetic lesion	365:380	a genetic lesion in the YFT1 gene	365:397	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	4	22	theme	fruit	590:594	arg1	phenotype					596:604	the yft1 firm fruit phenotype	576:604	the yft1 firm fruit phenotype	576:604	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	2	23	theme	yellow	320:325	arg1	mutant					350:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	1	24	theme	economic	226:233	arg1	value					235:239	economic value	226:239	economic value	226:239	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	3	25	theme	wild	460:463	arg1	type					465:468	M82 wild type	456:468	M82 wild type	456:468	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	0	26	theme	Fruit	11:15	arg1	Firmness					17:24	Fruit Firmness	11:24	Fruit Firmness	11:24	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	6	27	theme	chemical	790:797	arg1	components					799:808	chemical components	790:808	chemical components	790:808	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	0	28	theme	tomato	98:103	arg1	Mutant					115:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	0	29	theme	Cell	27:30	arg1	Composition					37:47	Cell Wall Composition	27:47	Cell Wall Composition	27:47	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	0	30	theme	yft1	109:112	arg1	Mutant					115:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	the yellow fruit tomato 1 ( yft1) Mutant	81:120	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	1	31	theme	important	144:152	arg1	firmness					129:136	Fruit firmness	123:136	Fruit firmness	123:136	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	1	31	theme	important	144:152	arg1	trait					154:158	an important trait	141:158	an important trait	141:158	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	2	32	theme	yft1	344:347	arg1	mutant					350:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant	318:355	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	2	33	theme	YFT1	389:392	arg1	gene					394:397	the YFT1 gene	385:397	the YFT1 gene	385:397	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	6	34	theme	cells	783:787	arg1	components					799:808	chemical components	790:808	chemical components	790:808	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	34	theme	cells	783:787	arg1	activities					821:830	hydrolase activities	811:830	hydrolase activities	811:830	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	34	theme	cells	783:787	arg1	expression					837:846	expression	837:846	expression of genes encoding these hydrolases	837:881	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	34	theme	cells	783:787	arg1	structure					757:765	the structure	753:765	the structure of the pericarp cells	753:787	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	7	35	dep	genes	1043:1047	arg1	DEGs					1050:1053	DEGs	1050:1053	DEGs	1050:1053	This was further affirmed by RNA-seq analysis, where a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated) were identified between yft1 and cv.					
30545217	7	35	dep	genes	1043:1047	arg1	down-/upregulated					1063:1079	down-/upregulated	1063:1079	down-/upregulated	1063:1079	This was further affirmed by RNA-seq analysis, where a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated) were identified between yft1 and cv.					
30545217	4	36	theme	pectin	668:673	arg1	deposition					675:684	pectin deposition	668:684	pectin deposition	668:684	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	9	37	theme	ethylene-	1177:1185	arg1	transduction					1212:1223	ethylene- and auxin-related signal transduction	1177:1223	ethylene- and auxin-related signal transduction	1177:1223	These DEGs were mainly annotated as participating in ethylene- and auxin-related signal transduction, sugar metabolism, and photosynthesis.					
30545217	6	38	theme	pericarp	774:781	arg1	cells					783:787	the pericarp cells	770:787	the pericarp cells	770:787	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	10	39	theme	fruit	1342:1346	arg1	softening					1348:1356	fruit softening	1342:1356	fruit softening	1342:1356	This study provides new insights into the mechanism underlying the control of fruit softening.					
30545217	7	40	theme	genes	1043:1047	arg1	total					1005:1009	a total	1003:1009	a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated)	1003:1080	This was further affirmed by RNA-seq analysis, where a total of 183 differentially expressed genes (DEGs, 50/133 down-/upregulated) were identified between yft1 and cv.					
30545217	3	41	theme	red	475:477	arg1	stage					484:488	a red ripe stage	473:488	a red ripe stage	473:488	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	41	theme	red	475:477	arg1	post-anthesis					499:511	54 days post-anthesis	491:511	54 days post-anthesis (dpa)	491:517	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	0	42	theme	Wall	32:35	arg1	Composition					37:47	Cell Wall Composition	27:47	Cell Wall Composition	27:47	Changes in Fruit Firmness, Cell Wall Composition, and Transcriptional Profile in the yellow fruit tomato 1 ( yft1) Mutant.					
30545217	3	43	theme	ripe	479:482	arg1	stage					484:488	a red ripe stage	473:488	a red ripe stage	473:488	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	43	theme	ripe	479:482	arg1	post-anthesis					499:511	54 days post-anthesis	491:511	54 days post-anthesis (dpa)	491:517	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	1	44	from	trait	154:158	arg1	tomato					163:168	tomato	163:168	tomato ( Solanum lycopersicum)	163:192	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	6	45	theme	function	940:947	arg1	loss					927:930	the loss	923:930	the loss of YFT1 function	923:947	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	1	46	theme	precise	255:261	arg1	mechanism					263:271	the precise mechanism	251:271	the precise mechanism determining fruit softening	251:299	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	1	47	dep	tomato	163:168	arg1	lycopersicum					180:191	Solanum lycopersicum	172:191	Solanum lycopersicum	172:191	Fruit firmness is an important trait in tomato ( Solanum lycopersicum), associated with shelf life and economic value; however, the precise mechanism determining fruit softening remains elusive.					
30545217	4	48	theme	firm	585:588	arg1	phenotype					596:604	the yft1 firm fruit phenotype	576:604	the yft1 firm fruit phenotype	576:604	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	4	49	theme	yft1	580:583	arg1	phenotype					596:604	the yft1 firm fruit phenotype	576:604	the yft1 firm fruit phenotype	576:604	When softening was further dissected, it was found that the yft1 firm fruit phenotype correlated with a difference in cellulose, hemicellulose, and pectin deposition in the primary cell wall (PCW) compared to cv.					
30545217	6	50	theme	YFT1	935:938	arg1	function					940:947	YFT1 function	935:947	YFT1 function	935:947	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	2	51	from	lesion	375:380	arg1	gene					394:397	the YFT1 gene	385:397	the YFT1 gene	385:397	A yellow fruit tomato 1 ( yft1) mutant harbors a genetic lesion in the YFT1 gene and has significantly firmer fruit than those of the cv.					
30545217	6	52	theme	loss	927:930	arg1	result					913:918	a result	911:918	a result of the loss of YFT1 function	911:947	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	52	theme	loss	927:930	arg1	Alterations					738:748	Alterations	738:748	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases	738:881	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	9	53	theme	signal	1205:1210	arg1	transduction					1212:1223	ethylene- and auxin-related signal transduction	1177:1223	ethylene- and auxin-related signal transduction	1177:1223	These DEGs were mainly annotated as participating in ethylene- and auxin-related signal transduction, sugar metabolism, and photosynthesis.					
30545217	6	54	from	Alterations	738:748	arg1	components					799:808	chemical components	790:808	chemical components	790:808	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	54	from	Alterations	738:748	arg1	activities					821:830	hydrolase activities	811:830	hydrolase activities	811:830	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	54	from	Alterations	738:748	arg1	expression					837:846	expression	837:846	expression of genes encoding these hydrolases	837:881	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	6	54	from	Alterations	738:748	arg1	structure					757:765	the structure	753:765	the structure of the pericarp cells	753:787	Alterations in the structure of the pericarp cells, chemical components, hydrolase activities, and expression of genes encoding these hydrolases were all hypothesized to be a result of the loss of YFT1 function.					
30545217	3	55	theme	days	494:497	arg1	stage					484:488	a red ripe stage	473:488	a red ripe stage	473:488	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	55	theme	days	494:497	arg1	dpa					514:516	dpa	514:516	dpa	514:516	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	55	theme	days	494:497	arg1	post-anthesis					499:511	54 days post-anthesis	491:511	54 days post-anthesis (dpa)	491:517	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	56	dep	type	465:468	arg1	stage					484:488	a red ripe stage	473:488	a red ripe stage	473:488	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
30545217	3	56	dep	type	465:468	arg1	post-anthesis					499:511	54 days post-anthesis	491:511	54 days post-anthesis (dpa)	491:517	M82 wild type at a red ripe stage, 54 days post-anthesis (dpa).					
31652012	3	0	theme	optimal	546:552	arg1	conditions					565:574	the optimal extraction conditions	542:574	the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W)	542:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	2	1	theme	nonstarch	298:306	arg1	polysaccharide					308:321	a water-soluble nonstarch polysaccharide	282:321	a water-soluble nonstarch polysaccharide from RYRP	282:331	In this study, a water-soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic-assisted extraction method.					
31652012	0	2	theme	polysaccharides	83:97	arg1	extraction					11:20	Ultrasonic extraction	0:20	Ultrasonic extraction	0:20	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	0	2	theme	polysaccharides	83:97	arg1	characterization					34:49	structural characterization	23:49	structural characterization	23:49	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	0	2	theme	polysaccharides	83:97	arg1	bioactivities					56:68	bioactivities	56:68	bioactivities	56:68	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	6	3	theme	model	1172:1176	arg1	mice					1178:1181	gastrointestinal disorders model mice	1145:1181	gastrointestinal disorders model mice	1145:1181	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	2	4	theme	water-soluble	284:296	arg1	polysaccharide					308:321	a water-soluble nonstarch polysaccharide	282:321	a water-soluble nonstarch polysaccharide from RYRP	282:331	In this study, a water-soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic-assisted extraction method.					
31652012	0	5	theme	yeast	108:112	arg1	rice					114:117	red yeast rice	104:117	red yeast rice	104:117	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	4	6	theme	composition	743:753	arg1	analysis					755:762	monosaccharide composition analysis	728:762	monosaccharide composition analysis	728:762	Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.152:0.015:1:0.149.					
31652012	0	7	theme	red	104:106	arg1	rice					114:117	red yeast rice	104:117	red yeast rice	104:117	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	3	8	theme	extraction	630:639	arg1	°C					657:658	62 °C	654:658	62 °C	654:658	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	8	theme	extraction	630:639	arg1	temperature					641:651	extraction temperature	630:651	extraction temperature (62 °C)	630:659	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	4	9	theme	monosaccharide	728:741	arg1	analysis					755:762	monosaccharide composition analysis	728:762	monosaccharide composition analysis	728:762	Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.152:0.015:1:0.149.					
31652012	6	10	theme	gastrointestinal	1145:1160	arg1	disorders					1162:1170	gastrointestinal disorders	1145:1170	gastrointestinal disorders model mice	1145:1181	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	7	11	from	source	1222:1227	arg1	industries					1271:1280	the pharmaceutical and functional food industries	1232:1280	the pharmaceutical and functional food industries	1232:1280	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	3	12	theme	ultrasonic	692:701	arg1	power					703:707	ultrasonic power	692:707	ultrasonic power (200 W)	692:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	12	theme	ultrasonic	692:701	arg1	W					714:714	200 W	710:714	200 W	710:714	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	4	13	theme	0.152:0.015:1:0.149	865:883	arg1	ratio					856:860	a molar ratio	848:860	a molar ratio of 0.152:0.015:1:0.149	848:883	Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.152:0.015:1:0.149.					
31652012	3	14	theme	maximum	483:489	arg1	yield					502:506	the maximum extraction yield	479:506	the maximum extraction yield (3.37 ± 0.78%)	479:521	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	14	theme	maximum	483:489	arg1	%					520:520	3.37 ± 0.78%	509:520	3.37 ± 0.78%	509:520	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	5	15	theme	molecular	953:961	arg1	Da					1012:1013	about 3.49 × 103 Da	995:1013	about 3.49 × 103 Da	995:1013	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	5	15	theme	molecular	953:961	arg1	weight					963:968	the average molecular weight	941:968	the average molecular weight of the RYRP fraction	941:989	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	3	16	theme	extraction	491:500	arg1	yield					502:506	the maximum extraction yield	479:506	the maximum extraction yield (3.37 ± 0.78%)	479:521	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	16	theme	extraction	491:500	arg1	%					520:520	3.37 ± 0.78%	509:520	3.37 ± 0.78%	509:520	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	7	17	theme	potential	1212:1220	arg1	source					1222:1227	a potential source	1210:1227	a potential source in the pharmaceutical and functional food industries	1210:1280	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	7	17	theme	potential	1212:1220	arg1	RYRP					1184:1187	RYRP	1184:1187	RYRP	1184:1187	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	5	18	theme	RYRP	977:980	arg1	fraction					982:989	the RYRP fraction	973:989	the RYRP fraction	973:989	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	6	19	theme	in	1131:1132	arg1	effect					1124:1129	the gastrointestinal-protective effect	1092:1129	the gastrointestinal-protective effect in vivo	1092:1137	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	6	20	dep	in	1131:1132	arg1	vivo					1134:1137	vivo	1134:1137	vivo	1134:1137	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	0	21	theme	Ultrasonic	0:9	arg1	extraction					11:20	Ultrasonic extraction	0:20	Ultrasonic extraction	0:20	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	3	22	dep	RYRP	473:476	arg1	obtained					527:534	obtained	527:534	was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W)	523:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	0	23	theme	structural	23:32	arg1	characterization					34:49	structural characterization	23:49	structural characterization	23:49	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	5	24	theme	molecular	890:898	arg1	weight					900:905	The molecular weight	886:905	The molecular weight distribution analysis	886:927	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	3	25	theme	3.37	509:512	arg1	yield					502:506	the maximum extraction yield	479:506	the maximum extraction yield (3.37 ± 0.78%)	479:521	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	25	theme	3.37	509:512	arg1	%					520:520	3.37 ± 0.78%	509:520	3.37 ± 0.78%	509:520	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	1	26	theme	folk	238:241	arg1	meat					208:211	meat	208:211	meat	208:211	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	26	theme	folk	238:241	arg1	medicine					243:250	a folk medicine	236:250	a folk medicine for centuries	236:264	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	27	used	used	228:231	arg2	medicine					243:250	a folk medicine	236:250	a folk medicine for centuries	236:264	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	27	used	used	228:231	arg2	meat					208:211	meat	208:211	meat	208:211	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	3	28	theme	±	514:514	arg1	yield					502:506	the maximum extraction yield	479:506	the maximum extraction yield (3.37 ± 0.78%)	479:521	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	28	theme	±	514:514	arg1	%					520:520	3.37 ± 0.78%	509:520	3.37 ± 0.78%	509:520	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	2	29	theme	extraction	376:385	arg1	method					387:392	ultrasonic-assisted extraction method	356:392	ultrasonic-assisted extraction method	356:392	In this study, a water-soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic-assisted extraction method.					
31652012	4	30	theme	molar	850:854	arg1	ratio					856:860	a molar ratio	848:860	a molar ratio of 0.152:0.015:1:0.149	848:883	Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.152:0.015:1:0.149.					
31652012	3	31	theme	Box-Behnken	408:418	arg1	design					420:425	the Box-Behnken design	404:425	the Box-Behnken design	404:425	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	6	32	theme	gastrointestinal-protective	1096:1122	arg1	effect					1124:1129	the gastrointestinal-protective effect	1092:1129	the gastrointestinal-protective effect in vivo	1092:1137	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	6	33	theme	significant	1044:1054	arg1	activities					1068:1077	significant antioxidant activities	1044:1077	significant antioxidant activities in vitro	1044:1086	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	2	34	theme	ultrasonic-assisted	356:374	arg1	method					387:392	ultrasonic-assisted extraction method	356:392	ultrasonic-assisted extraction method	356:392	In this study, a water-soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic-assisted extraction method.					
31652012	5	35	theme	×	1006:1006	arg1	Da					1012:1013	about 3.49 × 103 Da	995:1013	about 3.49 × 103 Da	995:1013	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	5	35	theme	×	1006:1006	arg1	weight					963:968	the average molecular weight	941:968	the average molecular weight of the RYRP fraction	941:989	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	5	36	theme	average	945:951	arg1	Da					1012:1013	about 3.49 × 103 Da	995:1013	about 3.49 × 103 Da	995:1013	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	5	36	theme	average	945:951	arg1	weight					963:968	the average molecular weight	941:968	the average molecular weight of the RYRP fraction	941:989	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	7	37	theme	functional	1255:1264	arg1	industries					1271:1280	the pharmaceutical and functional food industries	1232:1280	the pharmaceutical and functional food industries	1232:1280	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	7	38	dep	pharmaceutical	1236:1249	arg1	food					1266:1269	food	1266:1269	food	1266:1269	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	5	39	theme	distribution	907:918	arg1	analysis					920:927	The molecular weight distribution analysis	886:927	The molecular weight distribution analysis	886:927	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	2	40	from	RYRP	328:331	arg1	polysaccharide					308:321	a water-soluble nonstarch polysaccharide	282:321	a water-soluble nonstarch polysaccharide from RYRP	282:331	In this study, a water-soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic-assisted extraction method.					
31652012	1	41	theme	Red	120:122	arg1	rice					130:133	Red yeast rice	120:133	Red yeast rice (RYRP)	120:140	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	41	theme	Red	120:122	arg1	RYRP					136:139	RYRP	136:139	RYRP	136:139	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	5	42	theme	weight	900:905	arg1	analysis					920:927	The molecular weight distribution analysis	886:927	The molecular weight distribution analysis	886:927	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	3	43	theme	raw	606:608	arg1	material					610:617	raw material	606:617	raw material	606:617	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	1	44	theme	yeast	124:128	arg1	rice					130:133	Red yeast rice	120:133	Red yeast rice (RYRP)	120:140	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	44	theme	yeast	124:128	arg1	RYRP					136:139	RYRP	136:139	RYRP	136:139	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	3	45	theme	extraction	662:671	arg1	time					673:676	extraction time	662:676	extraction time (75 Min)	662:685	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	45	theme	extraction	662:671	arg1	Min					682:684	75 Min	679:684	75 Min	679:684	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	0	46	from	rice	114:117	arg1	extraction					11:20	Ultrasonic extraction	0:20	Ultrasonic extraction	0:20	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	0	46	from	rice	114:117	arg1	characterization					34:49	structural characterization	23:49	structural characterization	23:49	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	0	46	from	rice	114:117	arg1	bioactivities					56:68	bioactivities	56:68	bioactivities	56:68	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	3	47	theme	extraction	554:563	arg1	conditions					565:574	the optimal extraction conditions	542:574	the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W)	542:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	6	48	dep	in	1079:1080	arg1	vitro					1082:1086	vitro	1082:1086	vitro	1082:1086	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	7	49	theme	pharmaceutical	1236:1249	arg1	industries					1271:1280	the pharmaceutical and functional food industries	1232:1280	the pharmaceutical and functional food industries	1232:1280	RYRP could be explored as a potential source in the pharmaceutical and functional food industries.					
31652012	3	50	dep	follows	579:585	arg1	W					714:714	200 W	710:714	200 W	710:714	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	temperature					641:651	extraction temperature	630:651	extraction temperature (62 °C)	630:659	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	ratio					588:592	ratio	588:592	ratio of water to raw material (40 mL/g)	588:627	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	time					673:676	extraction time	662:676	extraction time (75 Min)	662:685	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	mL/g					623:626	40 mL/g	620:626	40 mL/g	620:626	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	power					703:707	ultrasonic power	692:707	ultrasonic power (200 W)	692:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	°C					657:658	62 °C	654:658	62 °C	654:658	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	50	dep	follows	579:585	arg1	Min					682:684	75 Min	679:684	75 Min	679:684	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	0	51	theme	nonstarch	73:81	arg1	polysaccharides					83:97	nonstarch polysaccharides	73:97	nonstarch polysaccharides	73:97	Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice.					
31652012	3	52	theme	water	597:601	arg1	W					714:714	200 W	710:714	200 W	710:714	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	temperature					641:651	extraction temperature	630:651	extraction temperature (62 °C)	630:659	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	ratio					588:592	ratio	588:592	ratio of water to raw material (40 mL/g)	588:627	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	time					673:676	extraction time	662:676	extraction time (75 Min)	662:685	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	mL/g					623:626	40 mL/g	620:626	40 mL/g	620:626	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	power					703:707	ultrasonic power	692:707	ultrasonic power (200 W)	692:715	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	°C					657:658	62 °C	654:658	62 °C	654:658	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	3	52	theme	water	597:601	arg1	Min					682:684	75 Min	679:684	75 Min	679:684	By using the Box-Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W).					
31652012	6	53	theme	antioxidant	1056:1066	arg1	activities					1068:1077	significant antioxidant activities	1044:1077	significant antioxidant activities in vitro	1044:1086	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	5	54	theme	fraction	982:989	arg1	Da					1012:1013	about 3.49 × 103 Da	995:1013	about 3.49 × 103 Da	995:1013	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	5	54	theme	fraction	982:989	arg1	weight					963:968	the average molecular weight	941:968	the average molecular weight of the RYRP fraction	941:989	The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da.					
31652012	1	55	used	utilized	151:158	arg2	RYRP					136:139	RYRP	136:139	RYRP	136:139	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	1	55	used	utilized	151:158	arg2	rice					130:133	Red yeast rice	120:133	Red yeast rice (RYRP)	120:140	Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries.					
31652012	6	56	theme	in	1079:1080	arg1	activities					1068:1077	significant antioxidant activities	1044:1077	significant antioxidant activities in vitro	1044:1086	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31652012	6	57	theme	disorders	1162:1170	arg1	mice					1178:1181	gastrointestinal disorders model mice	1145:1181	gastrointestinal disorders model mice	1145:1181	Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders model mice.					
31102614	12	0	theme	rats	1773:1776	arg1	content					1743:1749	the NO content	1736:1749	the NO content of loperamide-induced rats to ameliorate constipation in the rats	1736:1815	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	5	1	theme	medical	445:451	arg1	studies					453:459	modern medical studies	438:459	modern medical studies	438:459	However, modern medical studies are limited and lacking on its therapeutic mechanism.					
31102614	10	2	theme	molecular	1259:1267	arg1	1.11 × 103 kDa					1302:1315	1.11 × 103 kDa	1302:1315	1.11 × 103 kDa	1302:1315	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	10	2	theme	molecular	1259:1267	arg1	weight					1269:1274	the average molecular weight	1247:1274	the average molecular weight of AABP	1247:1282	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	10	3	dep	RESULTS	1217:1223	arg1	determined					1288:1297	determined	1288:1297	was determined as 1.11 × 103 kDa	1284:1315	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	6	4	theme	current	537:543	arg1	study					545:549	This current study	532:549	This current study	532:549	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	6	5	dep	aimed	555:559	arg1	AIM					515:517	AIM	515:517	AIM OF THE STUDY	515:530	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	12	6	theme	NO	1740:1741	arg1	content					1743:1749	the NO content	1736:1749	the NO content of loperamide-induced rats to ameliorate constipation in the rats	1736:1815	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	6	7	theme	STUDY	526:530	arg1	AIM					515:517	AIM	515:517	AIM OF THE STUDY	515:530	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	2	8	theme	Chinese	179:185	arg1	Anemarrhena					204:214	Anemarrhena	204:214	Anemarrhena	204:214	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	2	8	theme	Chinese	179:185	arg1	medicine					194:201	a traditional Chinese herbal medicine	165:201	a traditional Chinese herbal medicine	165:201	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	6	9	theme	potential	616:624	arg1	mechanism					626:634	the potential mechanism	612:634	the potential mechanism of Anemarrhena asphodeloides Bge	612:667	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	15	10	theme	laxative	2108:2115	arg1	AABP					2094:2097	AABP	2094:2097	AABP	2094:2097	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	15	10	theme	laxative	2108:2115	arg1	ingredients					2124:2134	the laxative active ingredients	2104:2134	the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism	2104:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	12	11	theme	motilin	1621:1627	arg1	levels					1596:1601	the levels	1592:1601	the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP)	1592:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	4	12	theme	thousand	404:411	arg1	years					413:417	more than one thousand years	390:417	more than one thousand years	390:417	Simultaneously, it has been used to treat constipation for more than one thousand years in China.					
31102614	14	13	theme	c-Kit	1961:1965	arg1	levels					1946:1951	the levels	1942:1951	the levels of SCF, c-Kit, AQP3 and VIP	1942:1979	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	5	14	theme	therapeutic	492:502	arg1	mechanism					504:512	its therapeutic mechanism	488:512	its therapeutic mechanism	488:512	However, modern medical studies are limited and lacking on its therapeutic mechanism.					
31102614	0	15	from	Anemarrhena	72:82	arg1	polysaccharides					51:65	polysaccharides	51:65	polysaccharides from Anemarrhena	51:82	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	0	15	from	Anemarrhena	72:82	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	0	15	from	Anemarrhena	72:82	arg1	effects					40:46	laxative effects	31:46	laxative effects	31:46	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	4	16	from	constipation	373:384	arg1	China					422:426	China	422:426	China	422:426	Simultaneously, it has been used to treat constipation for more than one thousand years in China.					
31102614	9	17	theme	therapeutic	1046:1056	arg1	mechanism					1058:1066	the underlining therapeutic mechanism	1030:1066	the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting	1030:1214	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	7	18	theme	loperamide-induced	696:713	arg1	rats					728:731	loperamide-induced constipation rats	696:731	loperamide-induced constipation rats	696:731	polysaccharides (AABP) in loperamide-induced constipation rats.					
31102614	12	19	theme	intestinal	1696:1705	arg1	VIP					1716:1718	VIP	1716:1718	VIP	1716:1718	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	12	19	theme	intestinal	1696:1705	arg1	peptide					1707:1713	vasoactive intestinal peptide	1685:1713	vasoactive intestinal peptide (VIP)	1685:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	13	20	theme	ICAM-1	1882:1887	arg1	expressions					1897:1907	ICAM-1 protein expressions	1882:1907	ICAM-1 protein expressions	1882:1907	Whilst, AABP repaired the damaged colons by regulating PCNA and ICAM-1 protein expressions.					
31102614	8	21	theme	infrared	802:809	arg1	spectrum					811:818	infrared spectrum	802:818	infrared spectrum	802:818	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	22	dep	MATERIALS	734:742	arg1	structure					760:768	The structure	756:768	MATERIALS AND METHODS The structure of AABP	734:776	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	11	23	theme	liquid	1533:1538	arg1	HPLC					1556:1559	HPLC	1556:1559	HPLC	1556:1559	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	11	23	theme	liquid	1533:1538	arg1	chromatography					1540:1553	high-performance liquid chromatography	1516:1553	high-performance liquid chromatography (HPLC)	1516:1560	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	6	24	theme	asphodeloides	651:663	arg1	Bge					665:667	Anemarrhena asphodeloides Bge	639:667	Anemarrhena asphodeloides Bge	639:667	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	8	25	theme	performance	826:836	arg1	HPGPC					869:873	HPGPC	869:873	HPGPC	869:873	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	25	theme	performance	826:836	arg1	chromatography					853:866	high performance gel permeation chromatography	821:866	high performance gel permeation chromatography (HPGPC)	821:874	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	26	theme	performance	886:896	arg1	HPLC					921:924	HPLC	921:924	HPLC	921:924	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	26	theme	performance	886:896	arg1	chromatography					905:918	high performance liquid chromatography	881:918	high performance liquid chromatography (HPLC)	881:925	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	15	27	attach	isolated	2136:2143	arg2	ingredients					2124:2134	the laxative active ingredients	2104:2134	the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism	2104:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	15	27	attach	isolated	2136:2143	arg1	Bge.					2176:2179	Bge.	2176:2179	Bge.	2176:2179	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	15	27	attach	isolated	2136:2143	arg2	AABP					2094:2097	AABP	2094:2097	AABP	2094:2097	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	11	28	theme	primary	1322:1328	arg1	compositions					1345:1356	The primary monosaccharide compositions	1318:1356	The primary monosaccharide compositions	1318:1356	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	8	29	theme	permeation	842:851	arg1	HPGPC					869:873	HPGPC	869:873	HPGPC	869:873	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	29	theme	permeation	842:851	arg1	chromatography					853:866	high performance gel permeation chromatography	821:866	high performance gel permeation chromatography (HPGPC)	821:874	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	14	30	theme	VIP	1977:1979	arg1	levels					1946:1951	the levels	1942:1951	the levels of SCF, c-Kit, AQP3 and VIP	1942:1979	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	13	31	theme	protein	1889:1895	arg1	expressions					1897:1907	ICAM-1 protein expressions	1882:1907	ICAM-1 protein expressions	1882:1907	Whilst, AABP repaired the damaged colons by regulating PCNA and ICAM-1 protein expressions.					
31102614	14	32	theme	AQP3	1968:1971	arg1	levels					1946:1951	the levels	1942:1951	the levels of SCF, c-Kit, AQP3 and VIP	1942:1979	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	12	33	theme	5-hydroxytryptamine	1654:1672	arg1	levels					1596:1601	the levels	1592:1601	the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP)	1592:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	0	34	from	effects	40:46	arg1	Anemarrhena					72:82	Anemarrhena	72:82	Anemarrhena	72:82	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	13	35	theme	damaged	1844:1850	arg1	colons					1852:1857	the damaged colons	1840:1857	the damaged colons	1840:1857	Whilst, AABP repaired the damaged colons by regulating PCNA and ICAM-1 protein expressions.					
31102614	0	36	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	1	37	theme	loperamide-induced	106:123	arg1	rats					125:128	loperamide-induced rats	106:128	loperamide-induced rats	106:128	in loperamide-induced rats.					
31102614	12	38	theme	substance	1636:1644	arg1	P					1646:1646	substance P	1636:1646	substance P (SP)	1636:1651	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	12	38	theme	substance	1636:1644	arg1	SP					1649:1650	SP	1649:1650	SP	1649:1650	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	14	39	theme	SCF	1956:1958	arg1	levels					1946:1951	the levels	1942:1951	the levels of SCF, c-Kit, AQP3 and VIP	1942:1979	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	11	40	dep	analyzed	1363:1370	arg1	1					1466:1466	1	1466:1466	1	1466:1466	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	0	41	theme	polysaccharides	51:65	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	0	41	theme	polysaccharides	51:65	arg1	effects					40:46	laxative effects	31:46	laxative effects	31:46	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	9	42	theme	AABP	1071:1074	arg1	mechanism					1058:1066	the underlining therapeutic mechanism	1030:1066	the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting	1030:1214	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	43	theme	Real-time	928:936	arg1	reaction					968:975	Real-time quantitative polymerase chain reaction	928:975	Real-time quantitative polymerase chain reaction (PCR)	928:981	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	43	theme	Real-time	928:936	arg1	PCR					978:980	PCR	978:980	PCR	978:980	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	44	theme	western	1199:1205	arg1	blotting					1207:1214	western blotting	1199:1214	western blotting	1199:1214	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	12	45	theme	loperamide-induced	1754:1771	arg1	rats					1773:1776	loperamide-induced rats	1754:1776	loperamide-induced rats	1754:1776	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	9	46	theme	polymerase	951:960	arg1	reaction					968:975	Real-time quantitative polymerase chain reaction	928:975	Real-time quantitative polymerase chain reaction (PCR)	928:981	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	46	theme	polymerase	951:960	arg1	PCR					978:980	PCR	978:980	PCR	978:980	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	10	47	theme	present	1232:1238	arg1	study					1240:1244	the present study	1228:1244	the present study	1228:1244	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	9	48	theme	reaction	968:975	arg1	methods					998:1004	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods	928:1004	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods	928:1004	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	5	49	theme	modern	438:443	arg1	studies					453:459	modern medical studies	438:459	modern medical studies	438:459	However, modern medical studies are limited and lacking on its therapeutic mechanism.					
31102614	2	50	theme	ETHNOPHARMACOLOGICAL	131:150	arg1	RELEVANCE					152:160	ETHNOPHARMACOLOGICAL RELEVANCE	131:160	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena	131:214	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	10	51	theme	average	1251:1257	arg1	1.11 × 103 kDa					1302:1315	1.11 × 103 kDa	1302:1315	1.11 × 103 kDa	1302:1315	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	10	51	theme	average	1251:1257	arg1	weight					1269:1274	the average molecular weight	1247:1274	the average molecular weight of AABP	1247:1282	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	2	52	theme	herbal	187:192	arg1	Anemarrhena					204:214	Anemarrhena	204:214	Anemarrhena	204:214	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	2	52	theme	herbal	187:192	arg1	medicine					194:201	a traditional Chinese herbal medicine	165:201	a traditional Chinese herbal medicine	165:201	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	15	53	theme	active	2117:2122	arg1	AABP					2094:2097	AABP	2094:2097	AABP	2094:2097	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	15	53	theme	active	2117:2122	arg1	ingredients					2124:2134	the laxative active ingredients	2104:2134	the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism	2104:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	11	54	theme	D-galacturonic	1405:1418	arg1	acid					1420:1423	D-galacturonic acid	1405:1423	D-galacturonic acid	1405:1423	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	2	55	theme	traditional	167:177	arg1	Anemarrhena					204:214	Anemarrhena	204:214	Anemarrhena	204:214	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	2	55	theme	traditional	167:177	arg1	medicine					194:201	a traditional Chinese herbal medicine	165:201	a traditional Chinese herbal medicine	165:201	ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese herbal medicine, Anemarrhena asphodeloides Bge.					
31102614	15	56	theme	gastrointestinal	2236:2251	arg1	hormones					2253:2260	the gastrointestinal hormones	2232:2260	the gastrointestinal hormones	2232:2260	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	11	57	dep	1	1466:1466	arg1	0.04					1469:1472	0.04	1469:1472	0.04	1469:1472	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	6	58	theme	laxative	580:587	arg1	activities					589:598	the laxative activities	576:598	the laxative activities	576:598	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	8	59	theme	AABP	773:776	arg1	structure					760:768	The structure	756:768	MATERIALS AND METHODS The structure of AABP	734:776	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	7	60	dep	polysaccharides	670:684	arg1	rats					728:731	loperamide-induced constipation rats	696:731	loperamide-induced constipation rats	696:731	polysaccharides (AABP) in loperamide-induced constipation rats.					
31102614	11	61	theme	high-performance	1516:1531	arg1	HPLC					1556:1559	HPLC	1556:1559	HPLC	1556:1559	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	11	61	theme	high-performance	1516:1531	arg1	chromatography					1540:1553	high-performance liquid chromatography	1516:1553	high-performance liquid chromatography (HPLC)	1516:1560	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	7	62	theme	constipation	715:726	arg1	rats					728:731	loperamide-induced constipation rats	696:731	loperamide-induced constipation rats	696:731	polysaccharides (AABP) in loperamide-induced constipation rats.					
31102614	12	63	theme	vasoactive	1685:1694	arg1	VIP					1716:1718	VIP	1716:1718	VIP	1716:1718	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	12	63	theme	vasoactive	1685:1694	arg1	peptide					1707:1713	vasoactive intestinal peptide	1685:1713	vasoactive intestinal peptide (VIP)	1685:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	10	64	theme	AABP	1279:1282	arg1	1.11 × 103 kDa					1302:1315	1.11 × 103 kDa	1302:1315	1.11 × 103 kDa	1302:1315	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	10	64	theme	AABP	1279:1282	arg1	weight					1269:1274	the average molecular weight	1247:1274	the average molecular weight of AABP	1247:1282	RESULTS In the present study, the average molecular weight of AABP was determined as 1.11 × 103 kDa.					
31102614	12	65	theme	peptide	1707:1713	arg1	levels					1596:1601	the levels	1592:1601	the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP)	1592:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	12	66	theme	gastrin	1606:1612	arg1	levels					1596:1601	the levels	1592:1601	the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP)	1592:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	6	67	theme	Bge	665:667	arg1	mechanism					626:634	the potential mechanism	612:634	the potential mechanism of Anemarrhena asphodeloides Bge	612:667	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	0	68	from	properties	16:25	arg1	Anemarrhena					72:82	Anemarrhena	72:82	Anemarrhena	72:82	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	6	69	theme	Anemarrhena	639:649	arg1	Bge					665:667	Anemarrhena asphodeloides Bge	639:667	Anemarrhena asphodeloides Bge	639:667	AIM OF THE STUDY This current study was aimed to investigate the laxative activities and explore the potential mechanism of Anemarrhena asphodeloides Bge.					
31102614	8	70	theme	high	821:824	arg1	HPGPC					869:873	HPGPC	869:873	HPGPC	869:873	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	70	theme	high	821:824	arg1	chromatography					853:866	high performance gel permeation chromatography	821:866	high performance gel permeation chromatography (HPGPC)	821:874	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	71	theme	high	881:884	arg1	HPLC					921:924	HPLC	921:924	HPLC	921:924	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	71	theme	high	881:884	arg1	chromatography					905:918	high performance liquid chromatography	881:918	high performance liquid chromatography (HPLC)	881:925	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	11	72	theme	monosaccharide	1330:1343	arg1	compositions					1345:1356	The primary monosaccharide compositions	1318:1356	The primary monosaccharide compositions	1318:1356	The primary monosaccharide compositions were analyzed including D-mannose, L-rhamnose, D-galacturonic acid, D-glucose, D-galactose and L-arabinose (1, 0.04, 0.53, 0.11, 0.33, 0.25, respectively) by high-performance liquid chromatography (HPLC).					
31102614	9	73	theme	enzyme-linked	1112:1124	arg1	assay					1140:1144	enzyme-linked immunosorbent assay	1112:1144	enzyme-linked immunosorbent assay (ELISA)	1112:1152	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	73	theme	enzyme-linked	1112:1124	arg1	ELISA					1147:1151	ELISA	1147:1151	ELISA	1147:1151	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	8	74	theme	gel	838:840	arg1	HPGPC					869:873	HPGPC	869:873	HPGPC	869:873	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	74	theme	gel	838:840	arg1	chromatography					853:866	high performance gel permeation chromatography	821:866	high performance gel permeation chromatography (HPGPC)	821:874	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	75	theme	liquid	898:903	arg1	HPLC					921:924	HPLC	921:924	HPLC	921:924	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	8	75	theme	liquid	898:903	arg1	chromatography					905:918	high performance liquid chromatography	881:918	high performance liquid chromatography (HPLC)	881:925	MATERIALS AND METHODS The structure of AABP was determined by using infrared spectrum, high performance gel permeation chromatography (HPGPC), and high performance liquid chromatography (HPLC).					
31102614	15	76	dep	CONCLUSION	2047:2056	arg1	suggested					2079:2087	suggested	2079:2087	suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism	2079:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	9	77	link	enzyme-linked	1112:1124	arg1	assay					1140:1144	enzyme-linked immunosorbent assay	1112:1144	enzyme-linked immunosorbent assay (ELISA)	1112:1152	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	77	link	enzyme-linked	1112:1124	arg1	ELISA					1147:1151	ELISA	1147:1151	ELISA	1147:1151	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	14	78	theme	PGE2	2041:2044	arg1	expressions					2011:2021	the expressions	2007:2021	the expressions of AQP8, AQP4 and PGE2	2007:2044	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	14	79	theme	AQP4	2032:2035	arg1	expressions					2011:2021	the expressions	2007:2021	the expressions of AQP8, AQP4 and PGE2	2007:2044	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	0	80	theme	laxative	31:38	arg1	effects					40:46	laxative effects	31:46	laxative effects	31:46	Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge.					
31102614	9	81	dep	reaction	968:975	arg1	multitudinous					984:996	Real-time quantitative polymerase chain reaction (PCR), multitudinous	928:996	multitudinous	984:996	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	4	82	used	used	359:362	arg2	it					347:348	it	347:348	it	347:348	Simultaneously, it has been used to treat constipation for more than one thousand years in China.					
31102614	14	83	theme	AQP8	2026:2029	arg1	expressions					2011:2021	the expressions	2007:2021	the expressions of AQP8, AQP4 and PGE2	2007:2044	Additionally, AABP up-regulated the levels of SCF, c-Kit, AQP3 and VIP as well as down-regulated the expressions of AQP8, AQP4 and PGE2.					
31102614	9	84	theme	immunosorbent	1126:1138	arg1	assay					1140:1144	enzyme-linked immunosorbent assay	1112:1144	enzyme-linked immunosorbent assay (ELISA)	1112:1152	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	84	theme	immunosorbent	1126:1138	arg1	ELISA					1147:1151	ELISA	1147:1151	ELISA	1147:1151	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	15	85	theme	present	2062:2068	arg1	findings					2070:2077	The present findings	2058:2077	The present findings	2058:2077	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	12	86	theme	P	1646:1646	arg1	levels					1596:1601	the levels	1592:1601	the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP)	1592:1719	AABP significantly increased the levels of gastrin (Gas), motilin (MTL), substance P (SP), 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP), and decreased the NO content of loperamide-induced rats to ameliorate constipation in the rats.					
31102614	15	87	theme	intestinal	2299:2308	arg1	metabolism					2329:2338	the intestinal motility and water metabolism	2295:2338	the intestinal motility and water metabolism	2295:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	9	88	theme	chain	962:966	arg1	reaction					968:975	Real-time quantitative polymerase chain reaction	928:975	Real-time quantitative polymerase chain reaction (PCR)	928:981	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	88	theme	chain	962:966	arg1	PCR					978:980	PCR	978:980	PCR	978:980	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	89	theme	quantitative	938:949	arg1	reaction					968:975	Real-time quantitative polymerase chain reaction	928:975	Real-time quantitative polymerase chain reaction (PCR)	928:981	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	9	89	theme	quantitative	938:949	arg1	PCR					978:980	PCR	978:980	PCR	978:980	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	15	90	theme	water	2323:2327	arg1	metabolism					2329:2338	the intestinal motility and water metabolism	2295:2338	the intestinal motility and water metabolism	2295:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
31102614	9	91	theme	underlining	1034:1044	arg1	mechanism					1058:1066	the underlining therapeutic mechanism	1030:1066	the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting	1030:1214	Real-time quantitative polymerase chain reaction (PCR), multitudinous methods were adopted to explore the underlining therapeutic mechanism of AABP in treating constipation, including enzyme-linked immunosorbent assay (ELISA), histopathological, immunohistochemistry and western blotting.					
31102614	15	92	theme	motility	2310:2317	arg1	metabolism					2329:2338	the intestinal motility and water metabolism	2295:2338	the intestinal motility and water metabolism	2295:2338	CONCLUSION The present findings suggested that AABP were the laxative active ingredients isolated from Anemarrhena asphodeloides Bge., which could treat constipation through regulating the gastrointestinal hormones and neurotransmitters to improve the intestinal motility and water metabolism.					
29777809	0	0	theme	Morus	103:107	arg1	L					114:114	Morus alba L	103:114	Morus alba L	103:114	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	1	1	theme	extraction	249:258	arg1	use					236:238	the sequential use	221:238	the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography	221:313	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	6	2	theme	in	818:819	arg1	experiment					827:836	the in vitro experiment	814:836	the in vitro experiment	814:836	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	2	3	theme	FPA-4	352:356	arg1	contents					326:333	The sugar contents	316:333	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5	316:366	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	2	3	theme	FPA-4	352:356	arg1	%					394:394	75.70%	389:394	75.70%	389:394	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	0	4	theme	L	114:114	arg1	fruit					94:98	fruit	94:98	fruit of Morus alba L	94:114	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	1	5	theme	ethanol	261:267	arg1	precipitation					269:281	ethanol precipitation	261:281	ethanol precipitation	261:281	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	0	6	theme	alba	109:112	arg1	L					114:114	Morus alba L	103:114	Morus alba L	103:114	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	3	7	theme	Man	532:534	arg1	Glc					549:551	Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc	532:574	Glc	549:551	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	6	8	dep	in	818:819	arg1	vitro					821:825	vitro	821:825	vitro	821:825	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	1	9	theme	precipitation	269:281	arg1	use					236:238	the sequential use	221:238	the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography	221:313	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	6	10	theme	necrosis	956:963	arg1	factor-α					965:972	tumour necrosis factor-α	949:972	tumour necrosis factor-α	949:972	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	5	11	theme	FPA-1	687:691	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	11	theme	FPA-1	687:691	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	3	12	dep	Glc	549:551	arg1	Gal					554:556	Gal	554:556	Gal	554:556	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	12	dep	Glc	549:551	arg1	Fuc					572:574	Fuc	572:574	Fuc	572:574	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	12	dep	Glc	549:551	arg1	GlcA					537:540	Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc	532:574	GlcA	537:540	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	12	dep	Glc	549:551	arg1	GalA					543:546	Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc	532:574	GalA	543:546	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	12	dep	Glc	549:551	arg1	Xyl					559:561	Xyl	559:561	Xyl	559:561	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	12	dep	Glc	549:551	arg1	Ara					564:566	Ara	564:566	Ara	564:566	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	5	13	theme	FPA-4	701:705	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	13	theme	FPA-4	701:705	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	4	14	located	detected	613:620	arg2	amount					595:600	a small amount	587:600	a small amount of Rha	587:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	14	located	detected	613:620	arg1	FPA-1					625:629	FPA-1	625:629	FPA-1	625:629	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	14	located	detected	613:620	arg1	FPA-5					642:646	FPA-5	642:646	FPA-5	642:646	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	14	located	detected	613:620	arg2	Rha					605:607	Rha	605:607	Rha	605:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	14	located	detected	613:620	arg1	FPA-4					632:636	FPA-4	632:636	FPA-4	632:636	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	6	15	theme	reactive	978:985	arg1	species					994:1000	reactive oxygen species	978:1000	reactive oxygen species	978:1000	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	0	16	theme	Systematic	0:9	arg1	fractionation					11:23	Systematic fractionation	0:23	Systematic fractionation	0:23	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	1	17	theme	Fructus	199:205	arg1	fruit					212:216	Fructus mori fruit	199:216	Fructus mori fruit	199:216	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	5	18	theme	FPA-5	711:715	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	18	theme	FPA-5	711:715	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	1	19	theme	ion-exchange	287:298	arg1	chromatography					300:313	ion-exchange chromatography	287:313	ion-exchange chromatography	287:313	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	2	20	theme	sugar	320:324	arg1	contents					326:333	The sugar contents	316:333	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5	316:366	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	2	20	theme	sugar	320:324	arg1	%					394:394	75.70%	389:394	75.70%	389:394	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	6	21	theme	oxygen	987:992	arg1	species					994:1000	reactive oxygen species	978:1000	reactive oxygen species	978:1000	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	1	22	theme	chromatography	300:313	arg1	use					236:238	the sequential use	221:238	the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography	221:313	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	1	23	theme	fruit	212:216	arg1	polysaccharides					180:194	the polysaccharides	176:194	the polysaccharides of Fructus mori fruit	176:216	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	0	24	theme	water-soluble	50:62	arg1	polysaccharides					64:78	water-soluble polysaccharides	50:78	water-soluble polysaccharides	50:78	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	6	25	theme	highest	859:865	arg1	activity					867:874	the highest activity	855:874	the highest activity	855:874	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	4	26	theme	small	589:593	arg1	Rha					605:607	Rha	605:607	Rha	605:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	26	theme	small	589:593	arg1	amount					595:600	a small amount	587:600	a small amount of Rha	587:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	6	27	theme	tumour	949:954	arg1	factor-α					965:972	tumour necrosis factor-α	949:972	tumour necrosis factor-α	949:972	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	1	28	dep	Fructus	199:205	arg1	mori					207:210	mori	207:210	mori	207:210	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	5	29	theme	FPA-2	694:698	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	29	theme	FPA-2	694:698	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	3	30	theme	composition	453:463	arg1	analysis					465:472	Monosaccharide composition analysis	438:472	Monosaccharide composition analysis	438:472	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	0	31	theme	polysaccharides	64:78	arg1	immunoenhancement					29:45	immunoenhancement	29:45	immunoenhancement	29:45	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	0	31	theme	polysaccharides	64:78	arg1	fractionation					11:23	Systematic fractionation	0:23	Systematic fractionation	0:23	Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.					
29777809	2	32	theme	FPA-2	345:349	arg1	contents					326:333	The sugar contents	316:333	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5	316:366	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	2	32	theme	FPA-2	345:349	arg1	%					394:394	75.70%	389:394	75.70%	389:394	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	3	33	contain	contained	522:530	arg1	polysaccharides					496:510	all the polysaccharides	488:510	all the polysaccharides	488:510	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	3	33	contain	contained	522:530	arg2	Glc					549:551	Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc	532:574	Glc	549:551	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	1	34	theme	sequential	225:234	arg1	use					236:238	the sequential use	221:238	the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography	221:313	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
29777809	6	35	dep	enhancing	879:887	arg1	promoting					903:911	promoting	903:911	promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species	903:1000	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	2	36	theme	FPA-1	338:342	arg1	contents					326:333	The sugar contents	316:333	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5	316:366	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	2	36	theme	FPA-1	338:342	arg1	%					394:394	75.70%	389:394	75.70%	389:394	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	6	37	theme	nitric	935:940	arg1	oxide					942:946	nitric oxide	935:946	nitric oxide	935:946	According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-α and reactive oxygen species.					
29777809	5	38	theme	molecular	653:661	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	38	theme	molecular	653:661	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	4	39	theme	Rha	605:607	arg1	Rha					605:607	Rha	605:607	Rha	605:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	4	39	theme	Rha	605:607	arg1	amount					595:600	a small amount	587:600	a small amount of Rha	587:607	Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5.					
29777809	3	40	theme	Monosaccharide	438:451	arg1	analysis					465:472	Monosaccharide composition analysis	438:472	Monosaccharide composition analysis	438:472	Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc.					
29777809	5	41	theme	weight	663:668	arg1	10.6-49.1 kDa					722:734	10.6-49.1 kDa	722:734	10.6-49.1 kDa	722:734	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	5	41	theme	weight	663:668	arg1	distributions					670:682	The molecular weight distributions	649:682	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5	649:715	The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively.					
29777809	2	42	theme	FPA-5	362:366	arg1	contents					326:333	The sugar contents	316:333	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5	316:366	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	2	42	theme	FPA-5	362:366	arg1	%					394:394	75.70%	389:394	75.70%	389:394	The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively.					
29777809	1	43	theme	water	243:247	arg1	extraction					249:258	water extraction	243:258	water extraction	243:258	In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography.					
31756476	8	0	theme	great	1601:1605	arg1	potential					1607:1615	the great potential	1597:1615	the great potential of this biomass-based conductive composite in conductive material application	1597:1693	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	3	1	theme	conductive	791:800	arg1	composites					802:811	wire-shaped conductive composites	779:811	wire-shaped conductive composites	779:811	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	1	2	theme	composite	157:165	arg1	wires					167:171	conductive composite wires	146:171	conductive composite wires	146:171	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	2	3	theme	TOCNFs/MWCNTs/α-DECHNs	456:477	arg1	wires					489:493	The TOCNFs/MWCNTs/α-DECHNs composite wires	452:493	The TOCNFs/MWCNTs/α-DECHNs composite wires	452:493	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	5	4	theme	potential	985:993	arg1	four-probe					1024:1033	a four-probe	1022:1033	a four-probe	1022:1033	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	4	theme	potential	985:993	arg1	Nano					1015:1018	Zetasizer Nano	1005:1018	Zetasizer Nano	1005:1018	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	4	theme	potential	985:993	arg1	analyzer					995:1002	a zeta potential analyzer	978:1002	a zeta potential analyzer (Zetasizer Nano)	978:1019	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	4	theme	potential	985:993	arg1	workstation					1055:1065	an electrochemical workstation	1036:1065	an electrochemical workstation	1036:1065	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	1	5	theme	wires	167:171	arg1	series					136:141	a series	134:141	a series of conductive composite wires	134:171	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	8	6	theme	material	1674:1681	arg1	application					1683:1693	conductive material application	1663:1693	conductive material application	1663:1693	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	4	7	theme	wire-like	882:890	arg1	shape					892:896	a wire-like shape	880:896	a wire-like shape with a rough but tight surface	880:927	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	6	8	theme	impedance	1225:1233	arg1	contents					1299:1306	the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents	1197:1306	the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents	1197:1306	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	1	9	theme	TEMPO-oxidized	269:282	arg1	TOCNFs					306:311	TOCNFs	306:311	TOCNFs	306:311	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	9	theme	TEMPO-oxidized	269:282	arg1	nanofibers					294:303	TEMPO-oxidized cellulose nanofibers	269:303	TEMPO-oxidized cellulose nanofibers (TOCNFs)	269:312	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	8	10	theme	conductive	1663:1672	arg1	application					1683:1693	conductive material application	1663:1693	conductive material application	1663:1693	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	5	11	theme	electrochemical	1039:1053	arg1	analyzer					995:1002	a zeta potential analyzer	978:1002	a zeta potential analyzer (Zetasizer Nano)	978:1019	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	11	theme	electrochemical	1039:1053	arg1	workstation					1055:1065	an electrochemical workstation	1036:1065	an electrochemical workstation	1036:1065	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	11	theme	electrochemical	1039:1053	arg1	Fourier					1070:1076	a Fourier	1068:1076	a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG)	1068:1185	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	6	12	theme	composite	1261:1269	arg1	wires					1271:1275	TOCNFs/MWCNTs/α-DECHNs composite wires	1238:1275	TOCNFs/MWCNTs/α-DECHNs composite wires	1238:1275	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	4	13	theme	composite	857:865	arg1	material					867:874	this conductive composite material	841:874	this conductive composite material	841:874	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	1	14	theme	cellulose	284:292	arg1	TOCNFs					306:311	TOCNFs	306:311	TOCNFs	306:311	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	14	theme	cellulose	284:292	arg1	nanofibers					294:303	TEMPO-oxidized cellulose nanofibers	269:303	TEMPO-oxidized cellulose nanofibers (TOCNFs)	269:312	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	15	theme	deacetylated	376:387	arg1	α-DECHNs					410:417	α-DECHNs	410:417	α-DECHNs	410:417	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	15	theme	deacetylated	376:387	arg1	nanofibers					398:407	partially deacetylated α-chitin nanofibers	366:407	partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process	366:449	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	5	16	theme	samples	948:954	arg1	properties					934:943	The properties	930:943	The properties of samples	930:954	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	2	17	theme	TOCNFs/MWCNTs	545:557	arg1	dispersion					559:568	the negatively charged TOCNFs/MWCNTs dispersion	522:568	the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion	522:616	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	1	18	theme	α-chitin	389:396	arg1	α-DECHNs					410:417	α-DECHNs	410:417	α-DECHNs	410:417	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	18	theme	α-chitin	389:396	arg1	nanofibers					398:407	partially deacetylated α-chitin nanofibers	366:407	partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process	366:449	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	2	19	theme	composite	479:487	arg1	wires					489:493	The TOCNFs/MWCNTs/α-DECHNs composite wires	452:493	The TOCNFs/MWCNTs/α-DECHNs composite wires	452:493	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	8	20	theme	prepared	1524:1531	arg1	wire					1543:1546	the prepared composite wire	1520:1546	the prepared composite wire	1520:1546	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	6	21	theme	AC	1222:1223	arg1	impedance					1225:1233	the AC impedance	1218:1233	the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs	1218:1297	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	0	22	theme	biomass-based	11:23	arg1	wires					35:39	Conductive biomass-based composite wires	0:39	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic	0:92	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	1	23	theme	nanofibers	398:407	arg1	dispersion					352:361	a dispersion	350:361	a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process	350:449	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	8	24	theme	biomass-based	1625:1637	arg1	composite					1650:1658	this biomass-based conductive composite	1620:1658	this biomass-based conductive composite	1620:1658	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	6	25	theme	conductivity	1201:1212	arg1	contents					1299:1306	the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents	1197:1306	the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents	1197:1306	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	8	26	dep	light	1552:1556	arg1	up					1558:1559	up	1558:1559	up	1558:1559	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	0	27	theme	Conductive	0:9	arg1	wires					35:39	Conductive biomass-based composite wires	0:39	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic	0:92	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	1	28	theme	nanofibers	294:303	arg1	dispersions					213:223	dispersions	213:223	dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents	213:343	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	8	29	from	potential	1607:1615	arg1	application					1683:1693	conductive material application	1663:1693	conductive material application	1663:1693	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	0	30	link	cross-linked	46:57	arg1	nanocellulose					67:79	cross-linked anionic nanocellulose	46:79	cross-linked anionic nanocellulose	46:79	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	8	31	theme	14.0 wt	1510:1516	arg1	%					1517:1517	14.0 wt%	1510:1517	14.0 wt%	1510:1517	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	4	32	theme	conductive	846:855	arg1	material					867:874	this conductive composite material	841:874	this conductive composite material	841:874	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	7	33	theme	%	1463:1463	arg1	%					1475:1475	3.0 wt% to 14.0 wt%	1457:1475	3.0 wt% to 14.0 wt%	1457:1475	The conductivity of the composite wire increases from 9.98 × 10-6 S∙cm-1 to 1.56 × 10-3 S∙cm-1 as the MWCNTs content raises from 3.0 wt% to 14.0 wt%.					
31756476	0	34	theme	composite	25:33	arg1	wires					35:39	Conductive biomass-based composite wires	0:39	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic	0:92	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	6	35	with	impedance	1225:1233	arg1	MWCNTs					1292:1297	different MWCNTs	1282:1297	different MWCNTs	1282:1297	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	2	36	theme	α-DECHNs	598:605	arg1	dispersion					607:616	the positively charged α-DECHNs dispersion	575:616	the positively charged α-DECHNs dispersion	575:616	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	8	37	theme	5 V	1581:1583	arg1	voltage					1570:1576	a voltage	1568:1576	a voltage of 5 V	1568:1583	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	0	38	theme	cross-linked	46:57	arg1	nanocellulose					67:79	cross-linked anionic nanocellulose	46:79	cross-linked anionic nanocellulose	46:79	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	7	39	theme	wire	1362:1365	arg1	conductivity					1332:1343	The conductivity	1328:1343	The conductivity of the composite wire	1328:1365	The conductivity of the composite wire increases from 9.98 × 10-6 S∙cm-1 to 1.56 × 10-3 S∙cm-1 as the MWCNTs content raises from 3.0 wt% to 14.0 wt%.					
31756476	2	40	theme	charged	590:596	arg1	dispersion					607:616	the positively charged α-DECHNs dispersion	575:616	the positively charged α-DECHNs dispersion	575:616	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	3	41	theme	electrostatic	720:732	arg1	heterocoagulation					747:763	heterocoagulation	747:763	heterocoagulation	747:763	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	3	41	theme	electrostatic	720:732	arg1	interaction					734:744	the electrostatic interaction	716:744	the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites	716:811	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	3	42	theme	charged	649:655	arg1	α-DECHNs					657:664	the positively charged α-DECHNs	634:664	the positively charged α-DECHNs	634:664	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	7	43	theme	composite	1352:1360	arg1	wire					1362:1365	the composite wire	1348:1365	the composite wire	1348:1365	The conductivity of the composite wire increases from 9.98 × 10-6 S∙cm-1 to 1.56 × 10-3 S∙cm-1 as the MWCNTs content raises from 3.0 wt% to 14.0 wt%.					
31756476	4	44	contain	has	876:878	arg1	material					867:874	this conductive composite material	841:874	this conductive composite material	841:874	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	4	44	contain	has	876:878	arg2	shape					892:896	a wire-like shape	880:896	a wire-like shape with a rough but tight surface	880:927	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	1	45	with	dispersions	213:223	arg1	contents					336:343	different MWCNTs contents	319:343	different MWCNTs contents	319:343	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	3	46	theme	TOCNFs/MWCNTs	693:705	arg1	contact					623:629	The contact	619:629	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs	619:705	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	3	47	theme	α-DECHNs	657:664	arg1	contact					623:629	The contact	619:629	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs	619:705	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	0	48	with	wires	35:39	arg1	cationic					85:92	cationic	85:92	cationic	85:92	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	0	48	with	wires	35:39	arg1	nanocellulose					67:79	cross-linked anionic nanocellulose	46:79	cross-linked anionic nanocellulose	46:79	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	1	49	theme	multi-wall	228:237	arg1	MWCNTs					257:262	MWCNTs	257:262	MWCNTs	257:262	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	49	theme	multi-wall	228:237	arg1	nanotubes					246:254	multi-wall carbon nanotubes	228:254	multi-wall carbon nanotubes (MWCNTs)	228:263	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	50	theme	different	319:327	arg1	contents					336:343	different MWCNTs contents	319:343	different MWCNTs contents	319:343	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	6	51	theme	different	1282:1290	arg1	MWCNTs					1292:1297	different MWCNTs	1282:1297	different MWCNTs	1282:1297	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	5	52	theme	thermogravimetric	1155:1171	arg1	TG					1183:1184	TG	1183:1184	TG	1183:1184	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	52	theme	thermogravimetric	1155:1171	arg1	analyzer					1173:1180	a thermogravimetric analyzer	1153:1180	a thermogravimetric analyzer (TG)	1153:1185	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	8	53	theme	conductive	1639:1648	arg1	composite					1650:1658	this biomass-based conductive composite	1620:1658	this biomass-based conductive composite	1620:1658	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	0	54	theme	anionic	59:65	arg1	nanocellulose					67:79	cross-linked anionic nanocellulose	46:79	cross-linked anionic nanocellulose	46:79	Conductive biomass-based composite wires with cross-linked anionic nanocellulose and cationic nanochitin as scaffolds.					
31756476	1	55	theme	MWCNTs	329:334	arg1	contents					336:343	different MWCNTs contents	319:343	different MWCNTs contents	319:343	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	7	56	dep	%	1475:1475	arg1	to					1465:1466	to	1465:1466	to	1465:1466	The conductivity of the composite wire increases from 9.98 × 10-6 S∙cm-1 to 1.56 × 10-3 S∙cm-1 as the MWCNTs content raises from 3.0 wt% to 14.0 wt%.					
31756476	8	57	theme	composite	1533:1541	arg1	wire					1543:1546	the prepared composite wire	1520:1546	the prepared composite wire	1520:1546	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	4	58	theme	tight	915:919	arg1	surface					921:927	a rough but tight surface	903:927	a rough but tight surface	903:927	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	8	59	theme	composite	1650:1658	arg1	potential					1607:1615	the great potential	1597:1615	the great potential of this biomass-based conductive composite in conductive material application	1597:1693	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	5	60	theme	Zetasizer	1005:1013	arg1	analyzer					995:1002	a zeta potential analyzer	978:1002	a zeta potential analyzer (Zetasizer Nano)	978:1019	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	60	theme	Zetasizer	1005:1013	arg1	Nano					1015:1018	Zetasizer Nano	1005:1018	Zetasizer Nano	1005:1018	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	7	61	theme	MWCNTs	1430:1435	arg1	content					1437:1443	the MWCNTs content	1426:1443	the MWCNTs content	1426:1443	The conductivity of the composite wire increases from 9.98 × 10-6 S∙cm-1 to 1.56 × 10-3 S∙cm-1 as the MWCNTs content raises from 3.0 wt% to 14.0 wt%.					
31756476	8	62	theme	MWCNTs	1487:1492	arg1	content					1494:1500	the MWCNTs content	1483:1500	the MWCNTs content	1483:1500	When the MWCNTs content reaches 14.0 wt%, the prepared composite wire can light up LED at a voltage of 5 V, indicating the great potential of this biomass-based conductive composite in conductive material application.					
31756476	6	63	theme	wires	1271:1275	arg1	conductivity					1201:1212	conductivity	1201:1212	conductivity	1201:1212	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	6	63	theme	wires	1271:1275	arg1	impedance					1225:1233	the AC impedance	1218:1233	the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs	1218:1297	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	4	64	theme	rough	905:909	arg1	surface					921:927	a rough but tight surface	903:927	a rough but tight surface	903:927	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	4	65	theme	SEM	818:820	arg1	analysis					822:829	The SEM analysis	814:829	The SEM analysis	814:829	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	1	66	theme	carbon	239:244	arg1	MWCNTs					257:262	MWCNTs	257:262	MWCNTs	257:262	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	66	theme	carbon	239:244	arg1	nanotubes					246:254	multi-wall carbon nanotubes	228:254	multi-wall carbon nanotubes (MWCNTs)	228:263	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	1	67	theme	drying	436:441	arg1	process					443:449	a drying process	434:449	a drying process	434:449	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	3	68	theme	charged	685:691	arg1	TOCNFs/MWCNTs					693:705	the negatively charged TOCNFs/MWCNTs	670:705	the negatively charged TOCNFs/MWCNTs	670:705	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	5	69	theme	zeta	980:983	arg1	four-probe					1024:1033	a four-probe	1022:1033	a four-probe	1022:1033	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	69	theme	zeta	980:983	arg1	Nano					1015:1018	Zetasizer Nano	1005:1018	Zetasizer Nano	1005:1018	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	69	theme	zeta	980:983	arg1	analyzer					995:1002	a zeta potential analyzer	978:1002	a zeta potential analyzer (Zetasizer Nano)	978:1019	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	69	theme	zeta	980:983	arg1	workstation					1055:1065	an electrochemical workstation	1036:1065	an electrochemical workstation	1036:1065	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	70	theme	X-ray	1121:1125	arg1	XRD					1143:1145	XRD	1143:1145	XRD	1143:1145	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	70	theme	X-ray	1121:1125	arg1	diffractometer					1127:1140	an X-ray diffractometer	1118:1140	an X-ray diffractometer (XRD)	1118:1146	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	1	71	theme	nanotubes	246:254	arg1	dispersions					213:223	dispersions	213:223	dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents	213:343	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
31756476	2	72	theme	charged	537:543	arg1	dispersion					559:568	the negatively charged TOCNFs/MWCNTs dispersion	522:568	the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion	522:616	The TOCNFs/MWCNTs/α-DECHNs composite wires were prepared by extruding the negatively charged TOCNFs/MWCNTs dispersion into the positively charged α-DECHNs dispersion.					
31756476	5	73	dep	Fourier	1070:1076	arg1	transform					1078:1086	transform	1078:1086	transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG)	1078:1185	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	6	74	theme	TOCNFs/MWCNTs/α-DECHNs	1238:1259	arg1	wires					1271:1275	TOCNFs/MWCNTs/α-DECHNs composite wires	1238:1275	TOCNFs/MWCNTs/α-DECHNs composite wires	1238:1275	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	6	75	with	conductivity	1201:1212	arg1	MWCNTs					1292:1297	different MWCNTs	1282:1297	different MWCNTs	1282:1297	Besides, the conductivity and the AC impedance of TOCNFs/MWCNTs/α-DECHNs composite wires with different MWCNTs contents were also analyzed.					
31756476	4	76	with	shape	892:896	arg1	surface					921:927	a rough but tight surface	903:927	a rough but tight surface	903:927	The SEM analysis indicates this conductive composite material has a wire-like shape with a rough but tight surface.					
31756476	3	77	theme	wire-shaped	779:789	arg1	composites					802:811	wire-shaped conductive composites	779:811	wire-shaped conductive composites	779:811	The contact of the positively charged α-DECHNs and the negatively charged TOCNFs/MWCNTs triggers the electrostatic interaction (heterocoagulation) resulting in wire-shaped conductive composites.					
31756476	5	78	theme	infrared	1088:1095	arg1	FTIR					1111:1114	FTIR	1111:1114	FTIR	1111:1114	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	5	78	theme	infrared	1088:1095	arg1	spectroscopy					1097:1108	infrared spectroscopy	1088:1108	infrared spectroscopy (FTIR)	1088:1115	The properties of samples were characterized by a zeta potential analyzer (Zetasizer Nano), a four-probe, an electrochemical workstation, a Fourier transform infrared spectroscopy (FTIR), an X-ray diffractometer (XRD), and a thermogravimetric analyzer (TG).					
31756476	1	79	theme	conductive	146:155	arg1	wires					167:171	conductive composite wires	146:171	conductive composite wires	146:171	In this study, a series of conductive composite wires were successfully prepared by combining dispersions of multi-wall carbon nanotubes (MWCNTs) and TEMPO-oxidized cellulose nanofibers (TOCNFs) with different MWCNTs contents into a dispersion of partially deacetylated α-chitin nanofibers (α-DECHNs) followed with a drying process.					
30590141	5	0	theme	0.5 mL	696:701	arg1	1 mg/mL					708:714	1 mg/mL	708:714	1 mg/mL	708:714	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	5	0	theme	0.5 mL	696:701	arg1	GPP					703:705	0.5 mL GPP	696:705	0.5 mL GPP (1 mg/mL) for 30 days	696:727	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	5	1	theme	GPP	703:705	arg1	administration					678:691	orally administration	671:691	orally administration of 0.5 mL GPP (1 mg/mL) for 30 days	671:727	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	2	2	from	acid	326:329	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	3	theme	molar	336:340	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	4	4	theme	structure	505:513	arg1	features					515:522	Primary structure features	497:522	Primary structure features of GPP	497:529	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	4	4	theme	structure	505:513	arg1	polysaccharide					555:568	a polysaccharide	553:568	a polysaccharide by FT-IR and NMR	553:585	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	2	5	dep	ratio	342:346	arg1	13.31					363:367	13.31	363:367	13.31	363:367	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	5	dep	ratio	342:346	arg1	0.91					383:386	0.91	383:386	0.91	383:386	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	5	dep	ratio	342:346	arg1	4.75					389:392	4.75	389:392	4.75	389:392	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	5	dep	ratio	342:346	arg1	1.07					377:380	1.07	377:380	1.07	377:380	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	5	dep	ratio	342:346	arg1	0.36					395:398	0.36	395:398	0.36	395:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	5	dep	ratio	342:346	arg1	7.34					357:360	7.34	357:360	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	6	from	acid	306:309	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	4	7	theme	GPP	527:529	arg1	features					515:522	Primary structure features	497:522	Primary structure features of GPP	497:529	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	4	7	theme	GPP	527:529	arg1	polysaccharide					555:568	a polysaccharide	553:568	a polysaccharide by FT-IR and NMR	553:585	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	8	8	dep	indicated	956:964	arg1	used					979:982	used	979:982	indicated GPP could be used as an effective ingredient to prevent and cure diabetes	956:1038	These results indicated GPP could be used as an effective ingredient to prevent and cure diabetes.					
30590141	5	9	theme	Fasting	588:594	arg1	sugar					602:606	Fasting blood sugar	588:606	Fasting blood sugar of diabetic mice	588:623	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	1	10	theme	Gynostemma	167:176	arg1	herb					191:194	Gynostemma pentaphyllum herb	167:194	Gynostemma pentaphyllum herb	167:194	In current study, a polysaccharide (GPP) was successfully extracted from Gynostemma pentaphyllum herb.					
30590141	1	11	theme	pentaphyllum	178:189	arg1	herb					191:194	Gynostemma pentaphyllum herb	167:194	Gynostemma pentaphyllum herb	167:194	In current study, a polysaccharide (GPP) was successfully extracted from Gynostemma pentaphyllum herb.					
30590141	0	12	theme	activity	14:21	arg1	evaluation					23:32	Anti-diabetic activity evaluation	0:32	Anti-diabetic activity evaluation of a polysaccharide	0:52	Anti-diabetic activity evaluation of a polysaccharide extracted from Gynostemma pentaphyllum.					
30590141	7	13	theme	protein	872:878	arg1	expression					880:889	the protein expression	868:889	the protein expression of GLUT2, but not the protein expression of SGLT1	868:939	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	1	14	theme	current	97:103	arg1	study					105:109	current study	97:109	current study	97:109	In current study, a polysaccharide (GPP) was successfully extracted from Gynostemma pentaphyllum herb.					
30590141	0	15	theme	Anti-diabetic	0:12	arg1	evaluation					23:32	Anti-diabetic activity evaluation	0:32	Anti-diabetic activity evaluation of a polysaccharide	0:52	Anti-diabetic activity evaluation of a polysaccharide extracted from Gynostemma pentaphyllum.					
30590141	2	16	from	galactose	256:264	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	5	17	theme	mice	620:623	arg1	sugar					602:606	Fasting blood sugar	588:606	Fasting blood sugar of diabetic mice	588:623	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	2	18	theme	galacturonic	293:304	arg1	acid					306:309	galacturonic acid	293:309	galacturonic acid	293:309	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	18	theme	galacturonic	293:304	arg1	rhamnose					235:242	rhamnose	235:242	rhamnose	235:242	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	7	19	theme	GLUT2	894:898	arg1	expression					880:889	the protein expression	868:889	the protein expression of GLUT2, but not the protein expression of SGLT1	868:939	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	4	20	theme	Primary	497:503	arg1	features					515:522	Primary structure features	497:522	Primary structure features of GPP	497:529	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	4	20	theme	Primary	497:503	arg1	polysaccharide					555:568	a polysaccharide	553:568	a polysaccharide by FT-IR and NMR	553:585	Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR.					
30590141	2	21	from	glucose	267:273	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	3	22	theme	Molecular	401:409	arg1	weight					411:416	Molecular weight	401:416	Molecular weight	401:416	Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively.					
30590141	3	22	theme	Molecular	401:409	arg1	Mw/Mn					438:442	Mw/Mn	438:442	Mw/Mn	438:442	Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively.					
30590141	0	23	theme	polysaccharide	39:52	arg1	evaluation					23:32	Anti-diabetic activity evaluation	0:32	Anti-diabetic activity evaluation of a polysaccharide	0:52	Anti-diabetic activity evaluation of a polysaccharide extracted from Gynostemma pentaphyllum.					
30590141	7	24	theme	glucose	838:844	arg1	absorption					846:855	the glucose absorption	834:855	the glucose absorption	834:855	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	6	25	theme	α-glucosidase	782:794	arg1	activity					796:803	α-glucosidase activity	782:803	α-glucosidase activity	782:803	GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity.					
30590141	7	26	theme	SGLT1	935:939	arg1	expression					921:930	the protein expression	909:930	the protein expression of SGLT1	909:939	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	6	27	from	effect	772:777	arg1	activity					796:803	α-glucosidase activity	782:803	α-glucosidase activity	782:803	GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity.					
30590141	5	28	theme	blood	596:600	arg1	sugar					602:606	Fasting blood sugar	588:606	Fasting blood sugar of diabetic mice	588:623	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	8	29	theme	effective	990:998	arg1	ingredient					1000:1009	an effective ingredient	987:1009	an effective ingredient to prevent and cure diabetes	987:1038	These results indicated GPP could be used as an effective ingredient to prevent and cure diabetes.					
30590141	2	30	theme	GPP	227:229	arg1	composition					212:222	Monosaccharide composition	197:222	Monosaccharide composition of GPP	197:229	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	7	31	theme	expression	921:930	arg1	expression					880:889	the protein expression	868:889	the protein expression of GLUT2, but not the protein expression of SGLT1	868:939	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	2	32	from	xylose	276:281	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	6	33	theme	inhibition	761:770	arg1	effect					772:777	a dose-dependent inhibition effect	744:777	a dose-dependent inhibition effect on α-glucosidase activity	744:803	GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity.					
30590141	5	34	theme	diabetic	611:618	arg1	mice					620:623	diabetic mice	611:623	diabetic mice	611:623	Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days.					
30590141	6	35	theme	dose-dependent	746:759	arg1	effect					772:777	a dose-dependent inhibition effect	744:777	a dose-dependent inhibition effect on α-glucosidase activity	744:803	GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity.					
30590141	2	36	theme	glucuronic	315:324	arg1	acid					326:329	glucuronic acid	315:329	glucuronic acid	315:329	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	36	theme	glucuronic	315:324	arg1	rhamnose					235:242	rhamnose	235:242	rhamnose	235:242	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	37	from	mannose	284:290	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	2	38	theme	Monosaccharide	197:210	arg1	composition					212:222	Monosaccharide composition	197:222	Monosaccharide composition of GPP	197:229	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	7	39	theme	protein	913:919	arg1	expression					921:930	the protein expression	909:930	the protein expression of SGLT1	909:939	Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1.					
30590141	2	40	theme	4.11	351:354	arg1	ratio					342:346	a molar ratio	334:346	a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36	334:398	Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36.					
30590141	0	41	theme	Gynostemma	69:78	arg1	pentaphyllum					80:91	Gynostemma pentaphyllum	69:91	Gynostemma pentaphyllum	69:91	Anti-diabetic activity evaluation of a polysaccharide extracted from Gynostemma pentaphyllum.					
30590141	3	42	theme	GPP	448:450	arg1	polydispersity					422:435	polydispersity	422:435	polydispersity	422:435	Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively.					
30590141	3	42	theme	GPP	448:450	arg1	weight					411:416	Molecular weight	401:416	Molecular weight	401:416	Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively.					
30590141	3	42	theme	GPP	448:450	arg1	Mw/Mn					438:442	Mw/Mn	438:442	Mw/Mn	438:442	Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively.					
31121093	5	0	from	average	1009:1015	arg1	accuracy					989:996	an accuracy	986:996	an accuracy of ∼94% on average	986:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	1	1	theme	biological	170:179	arg1	activities					181:190	potent biological activities	163:190	potent biological activities	163:190	Glycosaminoglycans (GAGs) are a class of polysaccharides with potent biological activities.					
31121093	5	2	with	enoxaparin	939:948	arg1	accuracy					989:996	an accuracy	986:996	an accuracy of ∼94% on average	986:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	3	3	theme	machine	527:533	arg1	learning					535:542	a support vector machine (SVM)-a machine learning	494:542	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	4	4	theme	low	756:758	arg1	impurities					797:806	as low as 0.8% (w/w) of chondroitin sulfate impurities	753:806	as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample	753:826	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	5	5	theme	%	1004:1004	arg1	accuracy					989:996	an accuracy	986:996	an accuracy of ∼94% on average	986:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	6	theme	low	951:953	arg1	heparin					921:927	unfractionated heparin	906:927	unfractionated heparin (UFH)	906:933	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	6	theme	low	951:953	arg1	heparin					972:978	low molecular weight heparin	951:978	low molecular weight heparin	951:978	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	4	7	theme	nanopore/SVM	610:621	arg1	technique					623:631	the nanopore/SVM technique	606:631	the nanopore/SVM technique	606:631	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	3	8	dep	-a	524:525	arg1	machine					511:517	support vector machine	496:517	support vector machine	496:517	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	2	9	theme	stereoisomers	301:313	arg1	charge					252:257	varied charge	245:257	varied charge	245:257	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	2	9	theme	stereoisomers	301:313	arg1	presence					280:287	presence	280:287	presence	280:287	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	2	9	theme	stereoisomers	301:313	arg1	polydispersity					260:273	polydispersity	260:273	polydispersity	260:273	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	2	9	theme	stereoisomers	301:313	arg1	composition					232:242	their complex and heterogeneous composition	200:242	their complex and heterogeneous composition	200:242	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	2	10	theme	current	377:383	arg1	techniques					396:405	current analytical techniques	377:405	current analytical techniques	377:405	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	1	11	theme	polysaccharides	142:156	arg1	Glycosaminoglycans					101:118	Glycosaminoglycans	101:118	Glycosaminoglycans (GAGs)	101:125	Glycosaminoglycans (GAGs) are a class of polysaccharides with potent biological activities.					
31121093	1	11	theme	polysaccharides	142:156	arg1	class					133:137	a class	131:137	a class of polysaccharides with potent biological activities	131:190	Glycosaminoglycans (GAGs) are a class of polysaccharides with potent biological activities.					
31121093	8	12	theme	technique	1275:1283	arg1	potential					1245:1253	the potential	1241:1253	the potential of the nanopore/SVM technique to quantify and identify GAGs	1241:1313	Our studies demonstrate the potential of the nanopore/SVM technique to quantify and identify GAGs.					
31121093	4	13	theme	monodisperse	659:670	arg1	fragments					672:680	monodisperse fragments	659:680	monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%)	659:741	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	2	14	theme	isobaric	292:299	arg1	stereoisomers					301:313	isobaric stereoisomers	292:313	isobaric stereoisomers	292:313	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	5	15	from	accuracy	989:996	arg1	average					1009:1015	average	1009:1015	average	1009:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	3	16	theme	single	466:471	arg1	molecule					473:480	solid-state nanopores-a single molecule	442:480	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	3	17	theme	present	415:421	arg1	study					423:427	the present study	411:427	the present study	411:427	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	3	18	theme	molecule	473:480	arg1	algorithm					544:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	0	19	theme	Molecule	7:14	arg1	Identification					16:29	Single Molecule Identification	0:29	Single Molecule Identification	0:29	Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores.					
31121093	2	20	theme	GAG	332:334	arg1	samples					336:342	GAG samples	332:342	GAG samples	332:342	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	3	21	theme	solid-state	442:452	arg1	molecule					473:480	solid-state nanopores-a single molecule	442:480	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	0	22	theme	Single	0:5	arg1	Identification					16:29	Single Molecule Identification	0:29	Single Molecule Identification	0:29	Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores.					
31121093	1	23	with	class	133:137	arg1	activities					181:190	potent biological activities	163:190	potent biological activities	163:190	Glycosaminoglycans (GAGs) are a class of polysaccharides with potent biological activities.					
31121093	5	24	from	%	1004:1004	arg1	average					1009:1015	average	1009:1015	average	1009:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	3	25	theme	nanopores-a	454:464	arg1	molecule					473:480	solid-state nanopores-a single molecule	442:480	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	4	26	theme	heparin	813:819	arg1	sample					821:826	a heparin sample	811:826	a heparin sample	811:826	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	3	27	theme	GAGs	574:577	arg1	analysis					562:569	the analysis	558:569	the analysis of GAGs	558:577	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	3	28	theme	support	496:502	arg1	machine					511:517	support vector machine	496:517	support vector machine	496:517	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	5	29	theme	weight	965:970	arg1	heparin					921:927	unfractionated heparin	906:927	unfractionated heparin (UFH)	906:933	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	29	theme	weight	965:970	arg1	heparin					972:978	low molecular weight heparin	951:978	low molecular weight heparin	951:978	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	6	30	theme	a 5-Log	1110:1116	arg1	range					1126:1130	a 5-Log dynamic range	1110:1130	a 5-Log dynamic range	1110:1130	With a reference sample for calibration, a nanopore could achieve nanomolar sensitivity and a 5-Log dynamic range.					
31121093	3	31	theme	vector	504:509	arg1	machine					511:517	support vector machine	496:517	support vector machine	496:517	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	3	32	theme	-a	524:525	arg1	learning					535:542	a support vector machine (SVM)-a machine learning	494:542	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	8	33	theme	nanopore/SVM	1262:1273	arg1	technique					1275:1283	the nanopore/SVM technique	1258:1283	the nanopore/SVM technique	1258:1283	Our studies demonstrate the potential of the nanopore/SVM technique to quantify and identify GAGs.					
31121093	4	34	theme	heparin	685:691	arg1	fragments					672:680	monodisperse fragments	659:680	monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%)	659:741	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	35	theme	sulfate	709:715	arg1	fragments					672:680	monodisperse fragments	659:680	monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%)	659:741	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	3	36	theme	sensor with	482:492	arg1	algorithm					544:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	4	37	theme	chondroitin	697:707	arg1	sulfate					709:715	chondroitin sulfate	697:715	chondroitin sulfate	697:715	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	5	38	theme	nanopore/SVM	861:872	arg1	technique					874:882	the nanopore/SVM technique	857:882	the nanopore/SVM technique	857:882	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	2	39	theme	varied	245:250	arg1	charge					252:257	varied charge	245:257	varied charge	245:257	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	5	40	theme	unfractionated	906:919	arg1	heparin					921:927	unfractionated heparin	906:927	unfractionated heparin (UFH)	906:933	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	40	theme	unfractionated	906:919	arg1	UFH					930:932	UFH	930:932	UFH	930:932	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	40	theme	unfractionated	906:919	arg1	heparin					972:978	low molecular weight heparin	951:978	low molecular weight heparin	951:978	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	0	41	theme	Glycosaminoglycans	53:70	arg1	Identification					16:29	Single Molecule Identification	0:29	Single Molecule Identification	0:29	Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores.					
31121093	0	41	theme	Glycosaminoglycans	53:70	arg1	Quantification					35:48	Quantification	35:48	Quantification	35:48	Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores.					
31121093	5	42	with	heparin	921:927	arg1	accuracy					989:996	an accuracy	986:996	an accuracy of ∼94% on average	986:1015	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	1	43	theme	potent	163:168	arg1	activities					181:190	potent biological activities	163:190	potent biological activities	163:190	Glycosaminoglycans (GAGs) are a class of polysaccharides with potent biological activities.					
31121093	2	44	theme	considerable	350:361	arg1	challenges					363:372	considerable challenges	350:372	considerable challenges to current analytical techniques	350:405	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	2	45	theme	heterogeneous	218:230	arg1	composition					232:242	their complex and heterogeneous composition	200:242	their complex and heterogeneous composition	200:242	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	4	46	theme	chondroitin	777:787	arg1	sulfate					789:795	chondroitin sulfate	777:795	chondroitin sulfate	777:795	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	47	theme	high	722:725	arg1	accuracy					727:734	high accuracy	722:734	high accuracy (>90%)	722:741	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	47	theme	high	722:725	arg1	%					740:740	>90%	737:740	>90%	737:740	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	6	48	theme	nanomolar	1084:1092	arg1	sensitivity					1094:1104	nanomolar sensitivity	1084:1104	nanomolar sensitivity	1084:1104	With a reference sample for calibration, a nanopore could achieve nanomolar sensitivity and a 5-Log dynamic range.					
31121093	6	49	theme	dynamic	1118:1124	arg1	range					1126:1130	a 5-Log dynamic range	1110:1130	a 5-Log dynamic range	1110:1130	With a reference sample for calibration, a nanopore could achieve nanomolar sensitivity and a 5-Log dynamic range.					
31121093	4	50	with	fragments	672:680	arg1	accuracy					727:734	high accuracy	722:734	high accuracy (>90%)	722:741	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	50	with	fragments	672:680	arg1	%					740:740	>90%	737:740	>90%	737:740	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	6	51	theme	reference	1025:1033	arg1	sample					1035:1040	a reference sample	1023:1040	a reference sample for calibration	1023:1056	With a reference sample for calibration, a nanopore could achieve nanomolar sensitivity and a 5-Log dynamic range.					
31121093	4	52	from	impurities	797:806	arg1	sample					821:826	a heparin sample	811:826	a heparin sample	811:826	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	2	53	theme	samples	336:342	arg1	analysis					320:327	the analysis	316:327	the analysis of GAG samples	316:342	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	7	54	theme	multiple	1197:1204	arg1	nanopores					1206:1214	multiple nanopores	1197:1214	multiple nanopores	1197:1214	We were able to quantify heparin with reasonable accuracy using multiple nanopores.					
31121093	2	55	theme	complex	206:212	arg1	composition					232:242	their complex and heterogeneous composition	200:242	their complex and heterogeneous composition	200:242	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	3	56	theme	learning	535:542	arg1	algorithm					544:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm	442:552	In the present study, we combined solid-state nanopores-a single molecule sensor with a support vector machine (SVM)-a machine learning algorithm for the analysis of GAGs.					
31121093	0	57	theme	Solid-State	78:88	arg1	Nanopores					90:98	Solid-State Nanopores	78:98	Solid-State Nanopores	78:98	Single Molecule Identification and Quantification of Glycosaminoglycans Using Solid-State Nanopores.					
31121093	5	58	theme	molecular	955:963	arg1	heparin					921:927	unfractionated heparin	906:927	unfractionated heparin (UFH)	906:933	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	5	58	theme	molecular	955:963	arg1	heparin					972:978	low molecular weight heparin	951:978	low molecular weight heparin	951:978	In addition, the nanopore/SVM technique distinguished between unfractionated heparin (UFH) and enoxaparin (low molecular weight heparin) with an accuracy of ∼94% on average.					
31121093	4	59	theme	sulfate	789:795	arg1	sulfate					789:795	chondroitin sulfate	777:795	chondroitin sulfate	777:795	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	59	theme	sulfate	789:795	arg1	w/w					769:771	w/w	769:771	w/w	769:771	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	4	59	theme	sulfate	789:795	arg1	%					766:766	0.8%	763:766	0.8% (w/w) of chondroitin sulfate	763:795	Our results indicate that the nanopore/SVM technique could distinguish between monodisperse fragments of heparin and chondroitin sulfate with high accuracy (>90%), allowing as low as 0.8% (w/w) of chondroitin sulfate impurities in a heparin sample to be detected.					
31121093	2	60	theme	analytical	385:394	arg1	techniques					396:405	current analytical techniques	377:405	current analytical techniques	377:405	Due to their complex and heterogeneous composition, varied charge, polydispersity, and presence of isobaric stereoisomers, the analysis of GAG samples poses considerable challenges to current analytical techniques.					
31121093	7	61	theme	reasonable	1171:1180	arg1	accuracy					1182:1189	reasonable accuracy	1171:1189	reasonable accuracy using multiple nanopores	1171:1214	We were able to quantify heparin with reasonable accuracy using multiple nanopores.					
31751750	7	0	theme	composite	1179:1187	arg1	materials					1189:1197	the composite materials	1175:1197	the composite materials	1175:1197	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	5	1	from	concentration	804:816	arg1	composite					832:840	the composite	828:840	the composite	828:840	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	4	2	theme	morphology	694:703	arg1	properties					705:714	surface morphology properties	686:714	surface morphology properties	686:714	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	1	3	with	materials	303:311	arg1	properties					362:371	potentially superior mechanical and thermal properties	318:371	potentially superior mechanical and thermal properties for biomedical applications	318:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	0	4	theme	composites	86:95	arg1	properties					26:35	Mechanical and biological properties	0:35	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites	0:95	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	1	5	theme	superior	330:337	arg1	properties					362:371	potentially superior mechanical and thermal properties	318:371	potentially superior mechanical and thermal properties for biomedical applications	318:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	6	6	dep	composites	1073:1082	arg1	%					1093:1093	%	1093:1093	%	1093:1093	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	2	7	theme	chitin/PLA/nHAP	503:517	arg1	composites					519:528	chitin/PLA/nHAP composites	503:528	chitin/PLA/nHAP composites	503:528	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	1	8	theme	mechanical	339:348	arg1	properties					362:371	potentially superior mechanical and thermal properties	318:371	potentially superior mechanical and thermal properties for biomedical applications	318:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	1	9	link	waste-derived	202:214	arg1	chitin					216:221	lobster shell waste-derived chitin	188:221	lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	188:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	9	10	from	use	1468:1470	arg1	regeneration					1480:1491	bone regeneration	1475:1491	bone regeneration	1475:1491	These results reveal that the biocomposites would be suitable candidates for use in bone regeneration that are not exposed to excessive forces.					
31751750	2	11	theme	liquid	412:417	arg1	[C2mim					456:461	[C2mim	456:461	[C2mim	456:461	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	11	theme	liquid	412:417	arg1	acetate					447:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate	402:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc])	402:468	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	11	theme	liquid	412:417	arg1	solvent					484:490	a solvent	482:490	a solvent	482:490	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	8	12	theme	osteocyte	1310:1318	arg1	expression					1325:1334	osteocyte gene expression	1310:1334	osteocyte gene expression	1310:1334	The materials were shown to have no effect on osteocyte gene expression, as well as minimal cytotoxicity and biodegradability.					
31751750	1	13	theme	potential	163:171	arg1	development					173:183	the potential development	159:183	the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	159:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	1	14	theme	thermal	354:360	arg1	properties					362:371	potentially superior mechanical and thermal properties	318:371	potentially superior mechanical and thermal properties for biomedical applications	318:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	9	15	theme	bone	1475:1478	arg1	regeneration					1480:1491	bone regeneration	1475:1491	bone regeneration	1475:1491	These results reveal that the biocomposites would be suitable candidates for use in bone regeneration that are not exposed to excessive forces.					
31751750	5	16	theme	material	968:975	arg1	toughness					951:959	the toughness	947:959	the toughness of the material	947:975	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	5	16	theme	material	968:975	arg1	elongation					923:932	the elongation	919:932	the elongation at break	919:941	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	6	17	theme	composites	1073:1082	arg1	strength					1057:1064	strength	1057:1064	strength of the composites up to 140%	1057:1093	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	5	18	dep	80 wt	867:871	arg1	to					864:865	to	864:865	to	864:865	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	7	19	theme	cells	1257:1261	arg1	proliferation					1222:1234	proliferation	1222:1234	proliferation	1222:1234	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	7	19	theme	cells	1257:1261	arg1	growth					1211:1216	growth	1211:1216	growth	1211:1216	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	0	20	theme	Mechanical	0:9	arg1	properties					26:35	Mechanical and biological properties	0:35	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites	0:95	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	2	21	dep	[C2mim	456:461	arg1	[OAc					463:466	[OAc	463:466	[OAc	463:466	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	3	22	theme	polymer	562:568	arg1	concentrations					570:583	the polymer concentrations	558:583	the polymer concentrations	558:583	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	1	23	theme	lobster	188:194	arg1	chitin					216:221	lobster shell waste-derived chitin	188:221	lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	188:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	4	24	theme	thermal	746:752	arg1	techniques					783:792	different thermal and optical characterization techniques	736:792	different thermal and optical characterization techniques	736:792	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	0	25	theme	liquid	114:119	arg1	solutions					121:129	ionic liquid solutions	108:129	ionic liquid solutions	108:129	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	6	26	dep	%	1093:1093	arg1	140					1090:1092	140	1090:1092	140	1090:1092	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	3	27	theme	variation	545:553	arg1	effect					535:540	The effect	531:540	The effect of variation of the polymer concentrations on the conduct of the resulting composite	531:625	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	2	28	theme	1-ethyl-3-methylimidazolium	419:445	arg1	[C2mim					456:461	[C2mim	456:461	[C2mim	456:461	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	28	theme	1-ethyl-3-methylimidazolium	419:445	arg1	acetate					447:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate	402:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc])	402:468	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	28	theme	1-ethyl-3-methylimidazolium	419:445	arg1	solvent					484:490	a solvent	482:490	a solvent	482:490	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	0	29	theme	biological	15:24	arg1	properties					26:35	Mechanical and biological properties	0:35	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites	0:95	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	0	30	theme	ionic	108:112	arg1	solutions					121:129	ionic liquid solutions	108:129	ionic liquid solutions	108:129	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	2	31	theme	ionic	406:410	arg1	[C2mim					456:461	[C2mim	456:461	[C2mim	456:461	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	31	theme	ionic	406:410	arg1	acetate					447:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate	402:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc])	402:468	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	31	theme	ionic	406:410	arg1	solvent					484:490	a solvent	482:490	a solvent	482:490	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	4	32	theme	optical	758:764	arg1	characterization					766:781	optical characterization	758:781	optical characterization	758:781	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	6	33	from	up	1135:1136	arg1	increase					1103:1110	an increase	1100:1110	an increase in elongation at break up to 465%	1100:1144	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	6	33	from	up	1135:1136	arg1	elongation					1115:1124	elongation	1115:1124	elongation at break up to 465%	1115:1144	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	1	34	theme	waste-derived	202:214	arg1	chitin					216:221	lobster shell waste-derived chitin	188:221	lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	188:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	1	35	theme	biomedical	377:386	arg1	applications					388:399	biomedical applications	377:399	biomedical applications	377:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	6	36	dep	140	1090:1092	arg1	to					1087:1088	to	1087:1088	to	1087:1088	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	5	37	theme	tensile	879:885	arg1	strength					887:894	the tensile strength	875:894	the tensile strength	875:894	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	1	38	theme	chitin	216:221	arg1	development					173:183	the potential development	159:183	the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	159:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	8	39	theme	minimal	1348:1354	arg1	cytotoxicity					1356:1367	minimal cytotoxicity	1348:1367	minimal cytotoxicity	1348:1367	The materials were shown to have no effect on osteocyte gene expression, as well as minimal cytotoxicity and biodegradability.					
31751750	6	40	from	increase	1103:1110	arg1	up					1135:1136	break up	1129:1136	break up to 465%	1129:1144	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	6	40	from	increase	1103:1110	arg1	elongation					1115:1124	elongation	1115:1124	elongation at break up to 465%	1115:1144	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	9	41	theme	suitable	1444:1451	arg1	candidates					1453:1462	suitable candidates	1444:1462	suitable candidates for use in bone regeneration that are not exposed to excessive forces	1444:1532	These results reveal that the biocomposites would be suitable candidates for use in bone regeneration that are not exposed to excessive forces.					
31751750	9	41	theme	suitable	1444:1451	arg1	biocomposites					1421:1433	the biocomposites	1417:1433	the biocomposites	1417:1433	These results reveal that the biocomposites would be suitable candidates for use in bone regeneration that are not exposed to excessive forces.					
31751750	5	42	theme	PLA	821:823	arg1	concentration					804:816	the concentration	800:816	the concentration of PLA in the composite	800:840	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	3	43	theme	concentrations	570:583	arg1	variation					545:553	variation	545:553	variation of the polymer concentrations	545:583	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	8	44	theme	gene	1320:1323	arg1	expression					1325:1334	osteocyte gene expression	1310:1334	osteocyte gene expression	1310:1334	The materials were shown to have no effect on osteocyte gene expression, as well as minimal cytotoxicity and biodegradability.					
31751750	2	45	used	used	474:477	arg2	acetate					447:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate	402:453	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc])	402:468	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	45	used	used	474:477	arg2	solvent					484:490	a solvent	482:490	a solvent	482:490	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	2	45	used	used	474:477	arg2	[C2mim					456:461	[C2mim	456:461	[C2mim	456:461	The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was used as a solvent to prepare chitin/PLA/nHAP composites.					
31751750	6	46	theme	break	1129:1133	arg1	up					1135:1136	break up	1129:1136	break up to 465%	1129:1144	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	7	47	theme	osteocyte	1247:1255	arg1	cells					1257:1261	Ocy 454 osteocyte cells	1239:1261	Ocy 454 osteocyte cells	1239:1261	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	1	48	theme	new	299:301	arg1	materials					303:311	new materials	299:311	new materials with potentially superior mechanical and thermal properties for biomedical applications	299:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	3	49	from	effect	535:540	arg1	conduct					592:598	the conduct	588:598	the conduct of the resulting composite	588:625	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	3	50	theme	resulting	607:615	arg1	composite					617:625	the resulting composite	603:625	the resulting composite	603:625	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	4	51	theme	different	736:744	arg1	techniques					783:792	different thermal and optical characterization techniques	736:792	different thermal and optical characterization techniques	736:792	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	4	52	theme	characterization	766:781	arg1	techniques					783:792	different thermal and optical characterization techniques	736:792	different thermal and optical characterization techniques	736:792	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	9	53	theme	excessive	1517:1525	arg1	forces					1527:1532	excessive forces	1517:1532	excessive forces	1517:1532	These results reveal that the biocomposites would be suitable candidates for use in bone regeneration that are not exposed to excessive forces.					
31751750	3	54	theme	composite	617:625	arg1	conduct					592:598	the conduct	588:598	the conduct of the resulting composite	588:625	The effect of variation of the polymer concentrations on the conduct of the resulting composite was explored.					
31751750	8	55	contain	have	1292:1295	arg1	materials					1268:1276	The materials	1264:1276	The materials	1264:1276	The materials were shown to have no effect on osteocyte gene expression, as well as minimal cytotoxicity and biodegradability.					
31751750	8	55	contain	have	1292:1295	arg2	effect					1300:1305	no effect	1297:1305	no effect	1297:1305	The materials were shown to have no effect on osteocyte gene expression, as well as minimal cytotoxicity and biodegradability.					
31751750	7	56	theme	Ocy	1239:1241	arg1	cells					1257:1261	Ocy 454 osteocyte cells	1239:1261	Ocy 454 osteocyte cells	1239:1261	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	6	57	theme	hydroxyapatite	1018:1031	arg1	addition					1006:1013	The addition	1002:1013	The addition of hydroxyapatite	1002:1031	The addition of hydroxyapatite was observed to improve strength of the composites up to 140% with an increase in elongation at break up to 465%.					
31751750	0	58	theme	/hydroxyapatite	64:78	arg1	composites					86:95	chitin/polylactide (PLA)/hydroxyapatite (HAP) composites	40:95	chitin/polylactide (PLA)/hydroxyapatite (HAP) composites	40:95	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	0	58	theme	/hydroxyapatite	64:78	arg1	HAP					81:83	HAP	81:83	HAP	81:83	Mechanical and biological properties of chitin/polylactide (PLA)/hydroxyapatite (HAP) composites cast using ionic liquid solutions.					
31751750	1	59	theme	shell	196:200	arg1	chitin					216:221	lobster shell waste-derived chitin	188:221	lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications	188:399	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	4	60	theme	surface	686:692	arg1	properties					705:714	surface morphology properties	686:714	surface morphology properties	686:714	The detailed physico-mechanical, thermal and surface morphology properties were evaluated with different thermal and optical characterization techniques.					
31751750	5	61	from	break	937:941	arg1	toughness					951:959	the toughness	947:959	the toughness of the material	947:975	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	5	61	from	break	937:941	arg1	elongation					923:932	the elongation	919:932	the elongation at break	919:941	When the concentration of PLA in the composite was increased from 20 to 80 wt%, the tensile strength improved by ~77% while the elongation at break and the toughness of the material decreased significantly.					
31751750	7	62	theme	Cell	1147:1150	arg1	growth					1152:1157	Cell growth	1147:1157	Cell growth	1147:1157	Cell growth study show that the composite materials support the growth and proliferation of Ocy 454 osteocyte cells.					
31751750	1	63	theme	lactic	244:249	arg1	poly					239:242	poly	239:242	poly(lactic acid) (PLA)	239:261	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31751750	1	63	theme	lactic	244:249	arg1	acid					251:254	lactic acid	244:254	lactic acid	244:254	This research investigates the potential development of lobster shell waste-derived chitin reinforced with poly(lactic acid) (PLA) and nano-hydroxyapatite (nHAP) into new materials with potentially superior mechanical and thermal properties for biomedical applications.					
31295497	5	0	theme	oligosaccharide	728:742	arg1	fingerprints					744:755	direct ESI--MS oligosaccharide fingerprints	713:755	direct ESI--MS oligosaccharide fingerprints coupled with PCA	713:772	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	9	1	from	characterization	1495:1510	arg1	foods					1534:1538	foods	1534:1538	foods	1534:1538	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	9	1	from	characterization	1495:1510	arg1	plants					1544:1549	plants	1544:1549	plants	1544:1549	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	8	2	theme	series	1394:1399	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	2	theme	series	1394:1399	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	4	3	theme	molecular	635:643	arg1	distributions					652:664	molecular weight distributions	635:664	molecular weight distributions	635:664	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	6	4	theme	acid	953:956	arg1	hydrolysates					958:969	mild acid hydrolysates	948:969	mild acid hydrolysates	948:969	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	0	5	theme	fruit	109:113	arg1	bodies					115:120	cultured fruit bodies	100:120	cultured fruit bodies of Auricularia auricular-judae	100:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	7	6	theme	residues	1086:1093	arg1	sequence					1059:1066	sequence	1059:1066	sequence of monosaccharide residues in oligosaccharides	1059:1113	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	7	6	theme	residues	1086:1093	arg1	linkages					1046:1053	interglycosidic linkages	1030:1053	interglycosidic linkages	1030:1053	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	6	7	theme	peak	917:920	arg1	number					922:927	distinct peak number	908:927	distinct peak number	908:927	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	0	8	theme	cultured	100:107	arg1	bodies					115:120	cultured fruit bodies	100:120	cultured fruit bodies of Auricularia auricular-judae	100:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	6	9	theme	distinct	908:915	arg1	number					922:927	distinct peak number	908:927	distinct peak number	908:927	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	4	10	theme	weight	645:650	arg1	distributions					652:664	molecular weight distributions	635:664	molecular weight distributions	635:664	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	0	11	from	perception	58:67	arg1	wild					91:94	wild	91:94	wild	91:94	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	0	11	from	perception	58:67	arg1	bodies					115:120	cultured fruit bodies	100:120	cultured fruit bodies of Auricularia auricular-judae	100:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	8	12	theme	1	1220:1220	arg1	q ≥ 0					1232:1236	q ≥ 0	1232:1236	q ≥ 0	1232:1236	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	y					1210:1210	y	1210:1210	y	1210:1210	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	z = 0					1213:1217	z = 0	1213:1217	z = 0	1213:1217	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	n					1226:1226	n	1226:1226	n	1226:1226	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	w					1204:1204	w	1204:1204	w	1204:1204	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	p					1229:1229	p	1229:1229	p	1229:1229	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	12	theme	1	1220:1220	arg1	x					1207:1207	x	1207:1207	x	1207:1207	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	13	theme	linear	1136:1141	arg1	skeleton					1311:1318	the general skeleton	1299:1318	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	13	theme	linear	1136:1141	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	13	theme	linear	1136:1141	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	14	theme	B-type	1344:1349	arg1	ions					1351:1354	B-type ions	1344:1354	B-type ions	1344:1354	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	7	15	theme	monosaccharide	1071:1084	arg1	residues					1086:1093	monosaccharide residues	1071:1093	monosaccharide residues	1071:1093	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	8	16	dep	w	1204:1204	arg1	Hex1 → 4GlcA					1266:1277	Hex1 → 4GlcA	1266:1277	Hex1 → 4GlcA	1266:1277	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	16	dep	w	1204:1204	arg1	GlcA1 → 4Hex					1252:1263	GlcA1 → 4Hex	1252:1263	GlcA1 → 4Hex	1252:1263	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	16	dep	w	1204:1204	arg1	m					1223:1223	m	1223:1223	m	1223:1223	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	5	17	contain	have	774:777	arg1	fingerprints					744:755	direct ESI--MS oligosaccharide fingerprints	713:755	direct ESI--MS oligosaccharide fingerprints coupled with PCA	713:772	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	5	17	contain	have	774:777	arg2	ability					794:800	the remarkable ability	779:800	the remarkable ability to discriminate wild and cultured ones	779:839	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	3	18	theme	chromatographic	413:427	arg1	approach					452:459	a stepped chromatographic and mass spectrometric approach	403:459	a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs	403:507	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	5	19	theme	direct	713:718	arg1	fingerprints					744:755	direct ESI--MS oligosaccharide fingerprints	713:755	direct ESI--MS oligosaccharide fingerprints coupled with PCA	713:772	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	6	20	theme	cultured	889:896	arg1	AAPs					898:901	cultured AAPs	889:901	cultured AAPs	889:901	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	2	21	theme	separating	354:363	arg1	AAPs					379:382	separating and detecting AAPs	354:382	separating and detecting AAPs	354:382	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	4	22	theme	GC-MS	530:534	arg1	techniques					536:545	HPSEC-MALLS-RID and GC-MS techniques	510:545	HPSEC-MALLS-RID and GC-MS techniques	510:545	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	0	23	theme	Chromatography	0:13	arg1	approaches					43:52	Chromatography and mass spectrometry-based approaches	0:52	approaches	43:52	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	4	24	theme	cultured	612:619	arg1	AAPs					621:624	wild and cultured AAPs	603:624	wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions	603:696	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	5	25	theme	ESI--MS	720:726	arg1	fingerprints					744:755	direct ESI--MS oligosaccharide fingerprints	713:755	direct ESI--MS oligosaccharide fingerprints coupled with PCA	713:772	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	q ≥ 0					1232:1236	q ≥ 0	1232:1236	q ≥ 0	1232:1236	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	y					1210:1210	y	1210:1210	y	1210:1210	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	z = 0					1213:1217	z = 0	1213:1217	z = 0	1213:1217	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	n					1226:1226	n	1226:1226	n	1226:1226	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	w					1204:1204	w	1204:1204	w	1204:1204	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	p					1229:1229	p	1229:1229	p	1229:1229	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	26	dep	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1143:1202	arg1	x					1207:1207	x	1207:1207	x	1207:1207	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	9	27	theme	polysaccharides	1515:1529	arg1	characterization					1495:1510	comprehensive characterization	1481:1510	comprehensive characterization of polysaccharides in foods and plants	1481:1549	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	0	28	theme	spectrometry-based	24:41	arg1	approaches					43:52	Chromatography and mass spectrometry-based approaches	0:52	approaches	43:52	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	0	29	theme	auricular-judae	137:151	arg1	bodies					115:120	cultured fruit bodies	100:120	cultured fruit bodies of Auricularia auricular-judae	100:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	7	30	from	linkages	1046:1053	arg1	oligosaccharides					1098:1113	oligosaccharides	1098:1113	oligosaccharides	1098:1113	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	3	31	theme	stepped	405:411	arg1	approach					452:459	a stepped chromatographic and mass spectrometric approach	403:459	a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs	403:507	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	0	32	theme	Auricularia	125:135	arg1	auricular-judae					137:151	Auricularia auricular-judae	125:151	Auricularia auricular-judae	125:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	2	33	theme	technical	334:342	arg1	issues					344:349	technical issues	334:349	technical issues in separating and detecting AAPs	334:382	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	9	34	theme	established	1411:1421	arg1	approach					1468:1475	a feasible approach	1457:1475	a feasible approach for comprehensive characterization of polysaccharides in foods and plants	1457:1549	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	9	34	theme	established	1411:1421	arg1	workflow					1423:1430	The established workflow	1407:1430	The established workflow	1407:1430	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	1	35	theme	important	231:239	arg1	polysaccharides					182:196	Auricularia auricular-judae polysaccharides	154:196	Auricularia auricular-judae polysaccharides (AAPs)	154:203	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	35	theme	important	231:239	arg1	constituent					252:262	one important biological constituent	227:262	one important biological constituent	227:262	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	3	36	theme	AAPs	504:507	arg1	characterization					484:499	characterization	484:499	characterization	484:499	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	3	36	theme	AAPs	504:507	arg1	discrimination					465:478	discrimination	465:478	discrimination	465:478	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	8	37	theme	AAPs	1323:1326	arg1	skeleton					1311:1318	the general skeleton	1299:1318	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	37	theme	AAPs	1323:1326	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	37	theme	AAPs	1323:1326	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	5	38	theme	wild	818:821	arg1	ones					836:839	wild and cultured ones	818:839	wild and cultured ones	818:839	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	8	39	theme	cleavage	1378:1385	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	39	theme	cleavage	1378:1385	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	2	40	theme	Quality	265:271	arg1	analysis					273:280	Quality analysis	265:280	Quality analysis of A. auricular-judae	265:302	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	1	41	theme	biological	241:250	arg1	polysaccharides					182:196	Auricularia auricular-judae polysaccharides	154:196	Auricularia auricular-judae polysaccharides (AAPs)	154:203	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	41	theme	biological	241:250	arg1	constituent					252:262	one important biological constituent	227:262	one important biological constituent	227:262	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	3	42	theme	mass	433:436	arg1	approach					452:459	a stepped chromatographic and mass spectrometric approach	403:459	a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs	403:507	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	9	43	theme	feasible	1459:1466	arg1	workflow					1423:1430	The established workflow	1407:1430	The established workflow	1407:1430	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	9	43	theme	feasible	1459:1466	arg1	approach					1468:1475	a feasible approach	1457:1475	a feasible approach for comprehensive characterization of polysaccharides in foods and plants	1457:1549	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	4	44	theme	HPSEC-MALLS-RID	510:524	arg1	techniques					536:545	HPSEC-MALLS-RID and GC-MS techniques	510:545	HPSEC-MALLS-RID and GC-MS techniques	510:545	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	7	45	from	sequence	1059:1066	arg1	oligosaccharides					1098:1113	oligosaccharides	1098:1113	oligosaccharides	1098:1113	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	3	46	theme	spectrometric	438:450	arg1	approach					452:459	a stepped chromatographic and mass spectrometric approach	403:459	a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs	403:507	Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs.					
31295497	4	47	theme	wild	603:606	arg1	AAPs					621:624	wild and cultured AAPs	603:624	wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions	603:696	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	6	48	theme	mild	948:951	arg1	hydrolysates					958:969	mild acid hydrolysates	948:969	mild acid hydrolysates	948:969	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	8	49	theme	A-type	1387:1392	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	49	theme	A-type	1387:1392	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	2	50	theme	detecting	369:377	arg1	AAPs					379:382	separating and detecting AAPs	354:382	separating and detecting AAPs	354:382	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	9	51	theme	comprehensive	1481:1493	arg1	characterization					1495:1510	comprehensive characterization	1481:1510	comprehensive characterization of polysaccharides in foods and plants	1481:1549	The established workflow has been confirmed to be a feasible approach for comprehensive characterization of polysaccharides in foods and plants.					
31295497	4	52	theme	monosaccharide	670:683	arg1	compositions					685:696	monosaccharide compositions	670:696	monosaccharide compositions	670:696	HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions.					
31295497	5	53	theme	remarkable	783:792	arg1	ability					794:800	the remarkable ability	779:800	the remarkable ability to discriminate wild and cultured ones	779:839	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
31295497	2	54	theme	auricular-judae	288:302	arg1	analysis					273:280	Quality analysis	265:280	Quality analysis of A. auricular-judae	265:302	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	1	55	theme	Auricularia	154:164	arg1	polysaccharides					182:196	Auricularia auricular-judae polysaccharides	154:196	Auricularia auricular-judae polysaccharides (AAPs)	154:203	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	55	theme	Auricularia	154:164	arg1	constituent					252:262	one important biological constituent	227:262	one important biological constituent	227:262	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	55	theme	Auricularia	154:164	arg1	AAPs					199:202	AAPs	199:202	AAPs	199:202	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	6	56	contain	have	903:906	arg2	number					922:927	distinct peak number	908:927	distinct peak number	908:927	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	6	56	contain	have	903:906	arg1	wild					880:883	wild	880:883	wild	880:883	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	6	56	contain	have	903:906	arg1	AAPs					898:901	cultured AAPs	889:901	cultured AAPs	889:901	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	6	56	contain	have	903:906	arg2	intensities					933:943	intensities	933:943	intensities	933:943	HILIC-UPLC-ESI--MS has indicated that wild and cultured AAPs have distinct peak number and intensities in mild acid hydrolysates.					
31295497	8	57	theme	cross-ring	1367:1376	arg1	ions					1401:1404	cross-ring cleavage A-type series ions	1367:1404	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	57	theme	cross-ring	1367:1376	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	1	58	theme	auricular-judae	166:180	arg1	polysaccharides					182:196	Auricularia auricular-judae polysaccharides	154:196	Auricularia auricular-judae polysaccharides (AAPs)	154:203	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	58	theme	auricular-judae	166:180	arg1	constituent					252:262	one important biological constituent	227:262	one important biological constituent	227:262	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	1	58	theme	auricular-judae	166:180	arg1	AAPs					199:202	AAPs	199:202	AAPs	199:202	Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent.					
31295497	7	59	theme	interglycosidic	1030:1044	arg1	linkages					1046:1053	interglycosidic linkages	1030:1053	interglycosidic linkages	1030:1053	HILIC-UPLC-ESI--HCD-MS/MS was further applied in deducing interglycosidic linkages and sequence of monosaccharide residues in oligosaccharides.					
31295497	0	60	theme	polysaccharides	72:86	arg1	perception					58:67	perception	58:67	perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae	58:151	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	0	61	theme	mass	19:22	arg1	approaches					43:52	Chromatography and mass spectrometry-based approaches	0:52	approaches	43:52	Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.					
31295497	8	62	theme	general	1303:1309	arg1	skeleton					1311:1318	the general skeleton	1299:1318	the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions	1299:1404	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	8	62	theme	general	1303:1309	arg1	GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 					1143:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → 	1136:1202	linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA)	1136:1278	We demonstrate that linear GlcAw → Hexm → GlcAx → Hexn → GlcAy → Hexp → GlcAz → Hexq → (w, x, y, z = 0, 1; m, n, p, q ≥ 0; Hex1 → 6Hex, GlcA1 → 4Hex, Hex1 → 4GlcA) was constructed as the general skeleton of AAPs based on C- and B-type ions as well as cross-ring cleavage A-type series ions.					
31295497	2	63	from	issues	344:349	arg1	AAPs					379:382	separating and detecting AAPs	354:382	separating and detecting AAPs	354:382	Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs.					
31295497	5	64	theme	cultured	827:834	arg1	ones					836:839	wild and cultured ones	818:839	wild and cultured ones	818:839	Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled with PCA have the remarkable ability to discriminate wild and cultured ones.					
30537504	0	0	theme	antipruritic	85:96	arg1	emulsion					113:120	antifungal and antipruritic peppermint oil emulsion	70:120	antifungal and antipruritic peppermint oil emulsion	70:120	Exopolysaccharide from Bacillus vallismortis WF4 as an emulsifier for antifungal and antipruritic peppermint oil emulsion.					
30537504	4	1	theme	%	507:507	arg1	%					528:528	51.77%:20.82%:13.28%:14.13%	502:528	51.77%:20.82%:13.28%:14.13%	502:528	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	10	2	theme	incidents	1352:1360	arg1	number					1331:1336	the number	1327:1336	the number of scratching incidents	1327:1360	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	1	3	from	exopolysaccharides	183:200	arg1	EOs					227:229	EOs	227:229	EOs	227:229	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	3	from	exopolysaccharides	183:200	arg1	oils					221:224	essential oils	211:224	essential oils (EOs)	211:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	6	4	theme	emulsifying	605:615	arg1	activity					617:624	significant emulsifying activity	593:624	significant emulsifying activity	593:624	EPS had significant emulsifying activity to EOs at 1.0% (w/v).					
30537504	11	5	theme	EPS	1417:1419	arg1	application					1402:1412	the application	1398:1412	the application of EPS	1398:1419	This study laid the foundation for the application of EPS as an emulsifier and highlighted the application prospects of EPS in topical emulsion.					
30537504	4	6	theme	%	514:514	arg1	%					528:528	51.77%:20.82%:13.28%:14.13%	502:528	51.77%:20.82%:13.28%:14.13%	502:528	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	1	7	from	capacities	159:168	arg1	EOs					227:229	EOs	227:229	EOs	227:229	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	7	from	capacities	159:168	arg1	oils					221:224	essential oils	211:224	essential oils (EOs)	211:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	6	8	theme	significant	593:603	arg1	activity					617:624	significant emulsifying activity	593:624	significant emulsifying activity	593:624	EPS had significant emulsifying activity to EOs at 1.0% (w/v).					
30537504	10	9	theme	scratching	1341:1350	arg1	incidents					1352:1360	scratching incidents	1341:1360	scratching incidents	1341:1360	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	0	10	theme	oil	109:111	arg1	emulsion					113:120	antifungal and antipruritic peppermint oil emulsion	70:120	antifungal and antipruritic peppermint oil emulsion	70:120	Exopolysaccharide from Bacillus vallismortis WF4 as an emulsifier for antifungal and antipruritic peppermint oil emulsion.					
30537504	10	11	theme	pigs	1288:1291	arg1	threshold					1268:1276	the itch threshold	1259:1276	the itch threshold of guinea pigs to phosphate histamine	1259:1314	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	8	12	theme	activity	790:797	arg1	results					799:805	The antifungal activity results	775:805	The antifungal activity results	775:805	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	1	13	theme	antifungal	241:250	arg1	properties					269:278	the antifungal and antipruritic properties	237:278	the antifungal and antipruritic properties of the emulsion	237:294	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	0	14	theme	peppermint	98:107	arg1	emulsion					113:120	antifungal and antipruritic peppermint oil emulsion	70:120	antifungal and antipruritic peppermint oil emulsion	70:120	Exopolysaccharide from Bacillus vallismortis WF4 as an emulsifier for antifungal and antipruritic peppermint oil emulsion.					
30537504	7	15	with	emulsion	709:716	arg1	size					743:746	an average particle size	723:746	an average particle size of 16.3 μm	723:757	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	9	16	theme	propidium	1015:1023	arg1	PI					1033:1034	PI	1033:1034	PI	1033:1034	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	9	16	theme	propidium	1015:1023	arg1	iodide					1025:1030	propidium iodide	1015:1030	propidium iodide (PI) staining	1015:1044	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	10	17	theme	guinea	1281:1286	arg1	pigs					1288:1291	guinea pigs	1281:1291	guinea pigs	1281:1291	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	10	18	theme	anti-itching	1180:1191	arg1	test					1193:1196	The anti-itching test	1176:1196	The anti-itching test	1176:1196	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	11	19	theme	application	1458:1468	arg1	prospects					1470:1478	the application prospects	1454:1478	the application prospects of EPS in topical emulsion	1454:1505	This study laid the foundation for the application of EPS as an emulsifier and highlighted the application prospects of EPS in topical emulsion.					
30537504	8	20	theme	pure	908:911	arg1	16.0 mg/mL					920:929	16.0 mg/mL	920:929	16.0 mg/mL	920:929	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	20	theme	pure	908:911	arg1	PO					913:914	the pure PO	904:914	the pure PO	904:914	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	1	21	theme	bacterial	173:181	arg1	EPS					203:205	EPS	203:205	EPS	203:205	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	21	theme	bacterial	173:181	arg1	exopolysaccharides					183:200	bacterial exopolysaccharides	173:200	bacterial exopolysaccharides (EPS) on essential oils (EOs)	173:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	8	22	theme	PO	964:965	arg1	emulsion					952:959	the emulsion	948:959	the emulsion of PO	948:965	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	5	23	theme	Rheology	532:539	arg1	study					541:545	Rheology study	532:545	Rheology study	532:545	Rheology study shows shear thinning characteristics.					
30537504	8	24	theme	minimum	823:829	arg1	concentration					842:854	the minimum inhibitory concentration	819:854	the minimum inhibitory concentration (MIC) of the EPS-PO emulsion	819:883	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	24	theme	minimum	823:829	arg1	4.0 mg/mL					889:897	4.0 mg/mL	889:897	4.0 mg/mL	889:897	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	24	theme	minimum	823:829	arg1	MIC					857:859	MIC	857:859	MIC	857:859	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	1	25	theme	exopolysaccharides	183:200	arg1	capacities					159:168	emulsifying capacities	147:168	emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs)	147:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	25	theme	exopolysaccharides	183:200	arg1	properties					269:278	the antifungal and antipruritic properties	237:278	the antifungal and antipruritic properties of the emulsion	237:294	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	2	26	theme	marine	299:304	arg1	bacterium					306:314	A marine bacterium	297:314	A marine bacterium Bacillus vallismortis	297:336	A marine bacterium Bacillus vallismortis was identified and named the WF4 strain.					
30537504	3	27	theme	EPS	407:409	arg1	3.83 × 105 Da					415:427	3.83 × 105 Da	415:427	3.83 × 105 Da	415:427	The molecular weight of the EPS was 3.83 × 105 Da.					
30537504	3	27	theme	EPS	407:409	arg1	weight					393:398	The molecular weight	379:398	The molecular weight of the EPS	379:409	The molecular weight of the EPS was 3.83 × 105 Da.					
30537504	1	28	theme	antipruritic	256:267	arg1	properties					269:278	the antifungal and antipruritic properties	237:278	the antifungal and antipruritic properties of the emulsion	237:294	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	29	from	properties	269:278	arg1	EOs					227:229	EOs	227:229	EOs	227:229	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	29	from	properties	269:278	arg1	oils					221:224	essential oils	211:224	essential oils (EOs)	211:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	9	30	theme	EPS-PO	1113:1118	arg1	emulsion					1120:1127	the EPS-PO emulsion	1109:1127	the EPS-PO emulsion	1109:1127	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	2	31	theme	WF4	367:369	arg1	strain					371:376	the WF4 strain	363:376	the WF4 strain	363:376	A marine bacterium Bacillus vallismortis was identified and named the WF4 strain.					
30537504	7	32	theme	16.3 μm	751:757	arg1	size					743:746	an average particle size	723:746	an average particle size of 16.3 μm	723:757	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	5	33	theme	shear	553:557	arg1	characteristics					568:582	shear thinning characteristics	553:582	shear thinning characteristics	553:582	Rheology study shows shear thinning characteristics.					
30537504	7	34	theme	particle	734:741	arg1	size					743:746	an average particle size	723:746	an average particle size of 16.3 μm	723:757	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	5	35	theme	thinning	559:566	arg1	characteristics					568:582	shear thinning characteristics	553:582	shear thinning characteristics	553:582	Rheology study shows shear thinning characteristics.					
30537504	10	36	theme	phosphate	1296:1304	arg1	histamine					1306:1314	phosphate histamine	1296:1314	phosphate histamine	1296:1314	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	9	37	theme	electron	1059:1066	arg1	SEM					1080:1082	SEM	1080:1082	SEM	1080:1082	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	9	37	theme	electron	1059:1066	arg1	microscopy					1068:1077	Scanning electron microscopy	1050:1077	Scanning electron microscopy (SEM) observation	1050:1095	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	4	38	dep	mannose/glucose/xylose/arabinose	468:499	arg1	%					528:528	51.77%:20.82%:13.28%:14.13%	502:528	51.77%:20.82%:13.28%:14.13%	502:528	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	8	39	theme	antifungal	985:994	arg1	ability					996:1002	the antifungal ability	981:1002	the antifungal ability	981:1002	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	7	40	theme	prepared	652:659	arg1	stable					767:772	stable	767:772	stable	767:772	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	40	theme	prepared	652:659	arg1	emulsion					709:716	an O/W emulsion	702:716	an O/W emulsion	702:716	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	40	theme	prepared	652:659	arg1	emulsion					689:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	9	41	theme	iodide	1025:1030	arg1	staining					1037:1044	propidium iodide (PI) staining	1015:1044	propidium iodide (PI) staining	1015:1044	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	7	42	theme	EPS-peppermint	661:674	arg1	EPS-PO					681:686	EPS-PO	681:686	EPS-PO	681:686	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	42	theme	EPS-peppermint	661:674	arg1	oil					676:678	EPS-peppermint oil	661:678	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	1	43	theme	essential	211:219	arg1	EOs					227:229	EOs	227:229	EOs	227:229	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	43	theme	essential	211:219	arg1	oils					221:224	essential oils	211:224	essential oils (EOs)	211:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	44	theme	emulsion	287:294	arg1	capacities					159:168	emulsifying capacities	147:168	emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs)	147:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	44	theme	emulsion	287:294	arg1	properties					269:278	the antifungal and antipruritic properties	237:278	the antifungal and antipruritic properties of the emulsion	237:294	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	4	45	theme	monosaccharide	449:462	arg1	composition					434:444	The composition	430:444	The composition of monosaccharide	430:462	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	4	45	theme	monosaccharide	449:462	arg1	mannose/glucose/xylose/arabinose					468:499	mannose/glucose/xylose/arabinose	468:499	mannose/glucose/xylose/arabinose	468:499	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	11	46	theme	topical	1490:1496	arg1	emulsion					1498:1505	topical emulsion	1490:1505	topical emulsion	1490:1505	This study laid the foundation for the application of EPS as an emulsifier and highlighted the application prospects of EPS in topical emulsion.					
30537504	3	47	theme	molecular	383:391	arg1	3.83 × 105 Da					415:427	3.83 × 105 Da	415:427	3.83 × 105 Da	415:427	The molecular weight of the EPS was 3.83 × 105 Da.					
30537504	3	47	theme	molecular	383:391	arg1	weight					393:398	The molecular weight	379:398	The molecular weight of the EPS	379:409	The molecular weight of the EPS was 3.83 × 105 Da.					
30537504	7	48	theme	average	726:732	arg1	size					743:746	an average particle size	723:746	an average particle size of 16.3 μm	723:757	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	6	49	contain	had	589:591	arg2	activity					617:624	significant emulsifying activity	593:624	significant emulsifying activity	593:624	EPS had significant emulsifying activity to EOs at 1.0% (w/v).					
30537504	6	49	contain	had	589:591	arg1	EPS					585:587	EPS	585:587	EPS	585:587	EPS had significant emulsifying activity to EOs at 1.0% (w/v).					
30537504	1	50	from	oils	221:224	arg1	capacities					159:168	emulsifying capacities	147:168	emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs)	147:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	1	50	from	oils	221:224	arg1	properties					269:278	the antifungal and antipruritic properties	237:278	the antifungal and antipruritic properties of the emulsion	237:294	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	11	51	from	prospects	1470:1478	arg1	emulsion					1498:1505	topical emulsion	1490:1505	topical emulsion	1490:1505	This study laid the foundation for the application of EPS as an emulsifier and highlighted the application prospects of EPS in topical emulsion.					
30537504	9	52	theme	Candida	1153:1159	arg1	albicans					1161:1168	Candida albicans	1153:1168	Candida albicans (CA)	1153:1173	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	9	52	theme	Candida	1153:1159	arg1	CA					1171:1172	CA	1171:1172	CA	1171:1172	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	8	53	theme	EPS-PO	869:874	arg1	emulsion					876:883	the EPS-PO emulsion	865:883	the EPS-PO emulsion	865:883	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	4	54	theme	%	521:521	arg1	%					528:528	51.77%:20.82%:13.28%:14.13%	502:528	51.77%:20.82%:13.28%:14.13%	502:528	The composition of monosaccharide was mannose/glucose/xylose/arabinose (51.77%:20.82%:13.28%:14.13%).					
30537504	9	55	theme	albicans	1161:1168	arg1	membrane					1141:1148	the membrane	1137:1148	the membrane of Candida albicans (CA)	1137:1173	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	10	56	theme	EPS-PO	1214:1219	arg1	emulsion					1221:1228	the EPS-PO emulsion	1210:1228	the EPS-PO emulsion	1210:1228	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	11	57	theme	EPS	1483:1485	arg1	prospects					1470:1478	the application prospects	1454:1478	the application prospects of EPS in topical emulsion	1454:1505	This study laid the foundation for the application of EPS as an emulsifier and highlighted the application prospects of EPS in topical emulsion.					
30537504	9	58	theme	Scanning	1050:1057	arg1	SEM					1080:1082	SEM	1080:1082	SEM	1080:1082	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	9	58	theme	Scanning	1050:1057	arg1	microscopy					1068:1077	Scanning electron microscopy	1050:1077	Scanning electron microscopy (SEM) observation	1050:1095	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	8	59	theme	inhibitory	831:840	arg1	concentration					842:854	the minimum inhibitory concentration	819:854	the minimum inhibitory concentration (MIC) of the EPS-PO emulsion	819:883	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	59	theme	inhibitory	831:840	arg1	4.0 mg/mL					889:897	4.0 mg/mL	889:897	4.0 mg/mL	889:897	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	59	theme	inhibitory	831:840	arg1	MIC					857:859	MIC	857:859	MIC	857:859	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	7	60	theme	O/W	705:707	arg1	stable					767:772	stable	767:772	stable	767:772	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	60	theme	O/W	705:707	arg1	emulsion					709:716	an O/W emulsion	702:716	an O/W emulsion	702:716	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	60	theme	O/W	705:707	arg1	emulsion					689:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	0	61	theme	antifungal	70:79	arg1	emulsion					113:120	antifungal and antipruritic peppermint oil emulsion	70:120	antifungal and antipruritic peppermint oil emulsion	70:120	Exopolysaccharide from Bacillus vallismortis WF4 as an emulsifier for antifungal and antipruritic peppermint oil emulsion.					
30537504	10	62	theme	itch	1263:1266	arg1	threshold					1268:1276	the itch threshold	1259:1276	the itch threshold of guinea pigs to phosphate histamine	1259:1314	The anti-itching test showed that the EPS-PO emulsion could significantly increase the itch threshold of guinea pigs to phosphate histamine and reduce the number of scratching incidents.					
30537504	8	63	theme	emulsion	876:883	arg1	concentration					842:854	the minimum inhibitory concentration	819:854	the minimum inhibitory concentration (MIC) of the EPS-PO emulsion	819:883	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	63	theme	emulsion	876:883	arg1	4.0 mg/mL					889:897	4.0 mg/mL	889:897	4.0 mg/mL	889:897	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	8	63	theme	emulsion	876:883	arg1	MIC					857:859	MIC	857:859	MIC	857:859	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30537504	7	64	theme	oil	676:678	arg1	stable					767:772	stable	767:772	stable	767:772	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	64	theme	oil	676:678	arg1	emulsion					709:716	an O/W emulsion	702:716	an O/W emulsion	702:716	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	7	64	theme	oil	676:678	arg1	emulsion					689:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion	648:696	The prepared EPS-peppermint oil (EPS-PO) emulsion was an O/W emulsion with an average particle size of 16.3 μm and was stable.					
30537504	0	65	from	vallismortis	32:43	arg1	Exopolysaccharide					0:16	Exopolysaccharide	0:16	Exopolysaccharide from Bacillus vallismortis	0:43	Exopolysaccharide from Bacillus vallismortis WF4 as an emulsifier for antifungal and antipruritic peppermint oil emulsion.					
30537504	9	66	theme	microscopy	1068:1077	arg1	observation					1085:1095	Scanning electron microscopy (SEM) observation	1050:1095	Scanning electron microscopy (SEM) observation	1050:1095	Moreover, propidium iodide (PI) staining and Scanning electron microscopy (SEM) observation showed that the EPS-PO emulsion damaged the membrane of Candida albicans (CA).					
30537504	1	67	theme	emulsifying	147:157	arg1	capacities					159:168	emulsifying capacities	147:168	emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs)	147:230	The paper aims to study emulsifying capacities of bacterial exopolysaccharides (EPS) on essential oils (EOs), and the antifungal and antipruritic properties of the emulsion.					
30537504	8	68	theme	antifungal	779:788	arg1	results					799:805	The antifungal activity results	775:805	The antifungal activity results	775:805	The antifungal activity results showed that the minimum inhibitory concentration (MIC) of the EPS-PO emulsion was 4.0 mg/mL, and the pure PO was 16.0 mg/mL, indicating that the emulsion of PO could enhance the antifungal ability.					
30235870	5	0	theme	immunostimulatory	646:662	arg1	activities					664:673	immunostimulatory activities	646:673	immunostimulatory activities	646:673	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	7	1	theme	typical	994:1000	arg1	heteropolysaccharides					1009:1029	typical acidic heteropolysaccharides	994:1029	typical acidic heteropolysaccharides	994:1029	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	1	2	theme	herb	166:169	arg1	scaber					177:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	9	3	theme	immunosuppressed	1461:1476	arg1	mice					1478:1481	immunosuppressed mice	1461:1481	immunosuppressed mice	1461:1481	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	0	4	from	Effect	4:9	arg1	Properties					82:91	the Physicochemical and Biological Properties	47:91	the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	47:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	8	5	theme	greatest	1198:1205	arg1	potential					1225:1233	the greatest immunostimulatory potential	1194:1233	the greatest immunostimulatory potential	1194:1233	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	1	6	theme	Aster	171:175	arg1	scaber					177:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	7	7	theme	Fourier-transform	872:888	arg1	spectroscopy					899:910	Fourier-transform infrared spectroscopy	872:910	Fourier-transform infrared spectroscopy (FT-IR)	872:918	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	7	7	theme	Fourier-transform	872:888	arg1	FT-IR					913:917	FT-IR	913:917	FT-IR	913:917	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	7	8	theme	acidic	1002:1007	arg1	heteropolysaccharides					1009:1029	typical acidic heteropolysaccharides	994:1029	typical acidic heteropolysaccharides	994:1029	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	5	9	theme	hot	743:745	arg1	water					747:751	hot water	743:751	hot water extracted polysaccharide (ASWP)	743:783	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	4	10	theme	extract	519:525	arg1	-A					564:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	10	11	theme	scaber	1635:1640	arg1	hydrolysate					1614:1624	pectinase hydrolysate	1604:1624	pectinase hydrolysate of Aster scaber	1604:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	7	12	theme	infrared	890:897	arg1	spectroscopy					899:910	Fourier-transform infrared spectroscopy	872:910	Fourier-transform infrared spectroscopy (FT-IR)	872:918	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	7	12	theme	infrared	890:897	arg1	FT-IR					913:917	FT-IR	913:917	FT-IR	913:917	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	8	13	from	assays	1141:1146	arg1	macrophages					1160:1170	RAW264.7 macrophages	1151:1170	RAW264.7 macrophages	1151:1170	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	0	14	theme	Polysaccharides	96:110	arg1	Properties					82:91	the Physicochemical and Biological Properties	47:91	the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	47:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	9	15	theme	killer	1531:1536	arg1	activity					1548:1555	natural killer (NK) cell activity	1523:1555	natural killer (NK) cell activity	1523:1555	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	8	16	theme	immunostimulatory	1207:1223	arg1	potential					1225:1233	the greatest immunostimulatory potential	1194:1233	the greatest immunostimulatory potential	1194:1233	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	5	17	theme	physicochemical	614:628	arg1	properties					630:639	physicochemical properties	614:639	physicochemical properties	614:639	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	10	18	theme	immunostimulatory	1667:1683	arg1	properties					1685:1694	efficacious immunostimulatory properties	1655:1694	efficacious immunostimulatory properties	1655:1694	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	3	19	theme	enzyme-assisted	447:461	arg1	methods					463:469	enzyme-assisted methods	447:469	enzyme-assisted methods	447:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	0	20	theme	Aster	117:121	arg1	scaber					123:128	Aster scaber	117:128	Aster scaber	117:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	9	21	theme	normal	1374:1379	arg1	mice					1381:1384	normal mice	1374:1384	normal mice	1374:1384	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	2	22	theme	biological	272:281	arg1	effects					283:289	the biological effects	268:289	the biological effects of its polysaccharides	268:312	However, the biological effects of its polysaccharides are not well understood.					
30235870	10	23	theme	immune	1733:1738	arg1	stimulator					1740:1749	an immune stimulator	1730:1749	an immune stimulator	1730:1749	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	23	theme	immune	1733:1738	arg1	applications					1714:1725	potential applications	1704:1725	potential applications	1704:1725	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	7	24	theme	galacturonic	1095:1106	arg1	acid					1108:1111	galacturonic acid	1095:1111	galacturonic acid	1095:1111	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	7	24	theme	galacturonic	1095:1106	arg1	rhamnose					1050:1057	rhamnose	1050:1057	rhamnose	1050:1057	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	10	25	theme	pectinase	1604:1612	arg1	hydrolysate					1614:1624	pectinase hydrolysate	1604:1624	pectinase hydrolysate of Aster scaber	1604:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	26	theme	efficacious	1655:1665	arg1	properties					1685:1694	efficacious immunostimulatory properties	1655:1694	efficacious immunostimulatory properties	1655:1694	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	8	27	theme	productions	1278:1288	arg1	terms					1238:1242	terms	1238:1242	terms of nitric oxide (NO) and cytokine productions and phagocytic activity	1238:1312	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	3	28	theme	enhanced	392:399	arg1	properties					412:421	enhanced biological properties	392:421	enhanced biological properties from Aster scaber using enzyme-assisted methods	392:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	6	29	theme	highest	790:796	arg1	yield					798:802	The highest yield	786:802	The highest yield (3.8%)	786:809	The highest yield (3.8%) was achieved for ASEP-P extracted using pectinase digestion.					
30235870	6	29	theme	highest	790:796	arg1	%					808:808	3.8%	805:808	3.8%	805:808	The highest yield (3.8%) was achieved for ASEP-P extracted using pectinase digestion.					
30235870	3	30	with	polysaccharides	371:385	arg1	properties					412:421	enhanced biological properties	392:421	enhanced biological properties from Aster scaber using enzyme-assisted methods	392:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	8	31	theme	cytokine	1269:1276	arg1	productions					1278:1288	cytokine productions	1269:1288	cytokine productions	1269:1288	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	3	32	theme	novel	365:369	arg1	polysaccharides					371:385	novel polysaccharides	365:385	novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods	365:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	7	33	theme	composition	933:943	arg1	analysis					945:952	chemical composition analysis	924:952	chemical composition analysis	924:952	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	9	34	theme	lymphocyte	1497:1506	arg1	proliferation					1508:1520	lymphocyte proliferation	1497:1520	lymphocyte proliferation	1497:1520	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	9	35	theme	lymphocyte	1428:1437	arg1	proliferation					1439:1451	splenic lymphocyte proliferation	1420:1451	splenic lymphocyte proliferation	1420:1451	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	8	36	theme	phagocytic	1294:1303	arg1	activity					1305:1312	phagocytic activity	1294:1312	phagocytic activity	1294:1312	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	4	37	used	used	511:514	arg2	cellulase					481:489	cellulase	481:489	cellulase	481:489	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	4	37	used	used	511:514	arg2	Amylase					472:478	Amylase	472:478	Amylase	472:478	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	4	37	used	used	511:514	arg2	pectinase					496:504	pectinase	496:504	pectinase	496:504	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	10	38	attach	derived	1591:1597	arg2	ASEP-P					1584:1589	The ASEP-P	1580:1589	The ASEP-P derived from pectinase hydrolysate of Aster scaber	1580:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	38	attach	derived	1591:1597	arg1	hydrolysate					1614:1624	pectinase hydrolysate	1604:1624	pectinase hydrolysate of Aster scaber	1604:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	2	39	theme	polysaccharides	298:312	arg1	effects					283:289	the biological effects	268:289	the biological effects of its polysaccharides	268:312	However, the biological effects of its polysaccharides are not well understood.					
30235870	1	40	theme	anticancer	201:210	arg1	properties					247:256	anticancer, antioxidant, and immunomodulatory properties	201:256	anticancer, antioxidant, and immunomodulatory properties	201:256	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	8	41	theme	oxide	1254:1258	arg1	terms					1238:1242	terms	1238:1242	terms of nitric oxide (NO) and cytokine productions and phagocytic activity	1238:1312	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	9	42	theme	spleen	1403:1408	arg1	index					1410:1414	the spleen index	1399:1414	the spleen index	1399:1414	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	8	43	theme	Immunostimulatory	1114:1130	arg1	assays					1141:1146	Immunostimulatory activity assays	1114:1146	Immunostimulatory activity assays on RAW264.7 macrophages	1114:1170	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	0	44	from	Properties	82:91	arg1	scaber					123:128	Aster scaber	117:128	Aster scaber	117:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	9	45	theme	natural	1523:1529	arg1	NK					1539:1540	NK	1539:1540	NK	1539:1540	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	9	45	theme	natural	1523:1529	arg1	killer					1531:1536	natural killer	1523:1536	natural killer (NK) cell activity	1523:1555	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	0	46	theme	Extraction	33:42	arg1	Effect					4:9	The Effect	0:9	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	0:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	3	47	theme	Aster	428:432	arg1	scaber					434:439	Aster scaber	428:439	Aster scaber using enzyme-assisted methods	428:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	6	48	theme	pectinase	851:859	arg1	digestion					861:869	pectinase digestion	851:869	pectinase digestion	851:869	The highest yield (3.8%) was achieved for ASEP-P extracted using pectinase digestion.					
30235870	0	49	theme	Pectinase-Assisted	14:31	arg1	Extraction					33:42	Pectinase-Assisted Extraction	14:42	Pectinase-Assisted Extraction	14:42	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	4	50	theme	enzyme-assisted	527:541	arg1	-A					564:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	1	51	theme	antioxidant	213:223	arg1	properties					247:256	anticancer, antioxidant, and immunomodulatory properties	201:256	anticancer, antioxidant, and immunomodulatory properties	201:256	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	0	52	theme	Physicochemical	51:65	arg1	Properties					82:91	the Physicochemical and Biological Properties	47:91	the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	47:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	4	53	theme	polysaccharide	543:556	arg1	-A					564:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	5	54	theme	polysaccharides	682:696	arg1	activities					664:673	immunostimulatory activities	646:673	immunostimulatory activities	646:673	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	5	54	theme	polysaccharides	682:696	arg1	yields					606:611	yields	606:611	yields	606:611	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	5	54	theme	polysaccharides	682:696	arg1	properties					630:639	physicochemical properties	614:639	physicochemical properties	614:639	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	9	55	theme	cell	1543:1546	arg1	activity					1548:1555	natural killer (NK) cell activity	1523:1555	natural killer (NK) cell activity	1523:1555	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	9	56	theme	immune-enhancing	1346:1361	arg1	effects					1363:1369	immune-enhancing effects	1346:1369	immune-enhancing effects in normal mice	1346:1384	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	9	57	theme	splenic	1420:1426	arg1	proliferation					1439:1451	splenic lymphocyte proliferation	1420:1451	splenic lymphocyte proliferation	1420:1451	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	8	58	theme	activity	1132:1139	arg1	assays					1141:1146	Immunostimulatory activity assays	1114:1146	Immunostimulatory activity assays on RAW264.7 macrophages	1114:1170	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	0	59	from	scaber	123:128	arg1	Properties					82:91	the Physicochemical and Biological Properties	47:91	the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	47:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	0	59	from	scaber	123:128	arg1	Polysaccharides					96:110	Polysaccharides	96:110	Polysaccharides from Aster scaber	96:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	5	60	dep	yields	606:611	arg1	The					602:604	The	602:604	The	602:604	The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP).					
30235870	4	61	theme	ASEP	559:562	arg1	-A					564:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	extract enzyme-assisted polysaccharide (ASEP)-A	519:565	Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively.					
30235870	8	62	theme	RAW264.7	1151:1158	arg1	macrophages					1160:1170	RAW264.7 macrophages	1151:1170	RAW264.7 macrophages	1151:1170	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	9	63	from	effects	1363:1369	arg1	mice					1381:1384	normal mice	1374:1384	normal mice	1374:1384	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	1	64	theme	edible	135:140	arg1	herb					166:169	edible and medicinal perennial herb	135:169	herb	166:169	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	7	65	theme	chemical	924:931	arg1	analysis					945:952	chemical composition analysis	924:952	chemical composition analysis	924:952	Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid.					
30235870	8	66	theme	activity	1305:1312	arg1	terms					1238:1242	terms	1238:1242	terms of nitric oxide (NO) and cytokine productions and phagocytic activity	1238:1312	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	10	67	contain	has	1700:1702	arg2	applications					1714:1725	potential applications	1704:1725	potential applications	1704:1725	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	67	contain	has	1700:1702	arg1	ASEP-P					1584:1589	The ASEP-P	1580:1589	The ASEP-P derived from pectinase hydrolysate of Aster scaber	1580:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	67	contain	has	1700:1702	arg2	stimulator					1740:1749	an immune stimulator	1730:1749	an immune stimulator	1730:1749	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	3	68	from	scaber	434:439	arg1	properties					412:421	enhanced biological properties	392:421	enhanced biological properties from Aster scaber using enzyme-assisted methods	392:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	8	69	theme	nitric	1247:1252	arg1	oxide					1254:1258	nitric oxide	1247:1258	nitric oxide (NO)	1247:1263	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	8	69	theme	nitric	1247:1252	arg1	NO					1261:1262	NO	1261:1262	NO	1261:1262	Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity.					
30235870	0	70	theme	Biological	71:80	arg1	Properties					82:91	the Physicochemical and Biological Properties	47:91	the Physicochemical and Biological Properties of Polysaccharides from Aster scaber	47:128	The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber.					
30235870	1	71	contain	have	196:199	arg1	scaber					177:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber	131:182	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	1	71	contain	have	196:199	arg2	properties					247:256	anticancer, antioxidant, and immunomodulatory properties	201:256	anticancer, antioxidant, and immunomodulatory properties	201:256	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	9	72	theme	leukocyte	1562:1570	arg1	counts					1572:1577	leukocyte counts	1562:1577	leukocyte counts	1562:1577	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	1	73	theme	medicinal	146:154	arg1	herb					166:169	edible and medicinal perennial herb	135:169	herb	166:169	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	1	74	theme	immunomodulatory	230:245	arg1	properties					247:256	anticancer, antioxidant, and immunomodulatory properties	201:256	anticancer, antioxidant, and immunomodulatory properties	201:256	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	3	75	theme	biological	401:410	arg1	properties					412:421	enhanced biological properties	392:421	enhanced biological properties from Aster scaber using enzyme-assisted methods	392:469	Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods.					
30235870	9	76	theme	ASEP-P	1315:1320	arg1	administration					1322:1335	ASEP-P administration	1315:1335	ASEP-P administration	1315:1335	ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts.					
30235870	1	77	theme	perennial	156:164	arg1	herb					166:169	edible and medicinal perennial herb	135:169	herb	166:169	The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties.					
30235870	10	78	theme	potential	1704:1712	arg1	stimulator					1740:1749	an immune stimulator	1730:1749	an immune stimulator	1730:1749	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	78	theme	potential	1704:1712	arg1	applications					1714:1725	potential applications	1704:1725	potential applications	1704:1725	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30235870	10	79	theme	Aster	1629:1633	arg1	scaber					1635:1640	Aster scaber	1629:1640	Aster scaber	1629:1640	The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.					
30465838	2	0	theme	proteins	449:456	arg1	acids					432:436	uronic acids	425:436	uronic acids (9.2%)	425:443	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	proteins					449:456	proteins	449:456	proteins (3.7%)	449:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	groups					410:415	sulfated groups	401:415	sulfated groups (6.6%)	401:422	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	%					421:421	6.6%	418:421	6.6%	418:421	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	%					442:442	9.2%	439:442	9.2%	439:442	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	amounts					390:396	the highest amounts	378:396	the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%)	378:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	0	theme	proteins	449:456	arg1	%					462:462	3.7%	459:462	3.7%	459:462	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	1	1	theme	novel	162:166	arg1	polysaccharides					168:182	novel polysaccharides	162:182	novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP)	162:236	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	0	2	theme	polysaccharides	88:102	arg1	activities					65:74	antioxidant and antibacterial activities	35:74	antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle	35:144	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	7	3	theme	DEAE-cellulose	1041:1054	arg1	column					1056:1061	DEAE-cellulose column	1041:1061	DEAE-cellulose column	1041:1061	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	8	4	theme	sulfated	1220:1227	arg1	fraction					1229:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	8	5	theme	last	1207:1210	arg1	fraction					1229:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	0	6	theme	cuttlefish	119:128	arg1	skin					130:133	cuttlefish skin	119:133	cuttlefish skin	119:133	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	6	7	dep	in	977:978	arg1	vitro					980:984	vitro	980:984	vitro	980:984	In addition, they exhibited strong antioxidant activities as showed by various in vitro tests.					
30465838	7	8	theme	polysaccharides	1021:1035	arg1	Fractionation					993:1005	Fractionation	993:1005	Fractionation	993:1005	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	4	9	theme	acid	668:671	arg1	components					689:698	major components	683:698	major components	683:698	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	9	theme	acid	668:671	arg1	presence					616:623	the presence	612:623	the presence of glucuronic acid (GlcA) and galacturonic acid (GalA)	612:678	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	5	10	theme	antibacterial	833:845	arg1	activity					847:854	important antibacterial activity	823:854	important antibacterial activity	823:854	Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria.					
30465838	9	11	theme	cuttlefish	1413:1422	arg1	fractions					1450:1458	their fractions	1444:1458	their fractions	1444:1458	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	9	11	theme	cuttlefish	1413:1422	arg1	polysaccharides					1424:1438	cuttlefish polysaccharides	1413:1438	cuttlefish polysaccharides	1413:1438	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	9	11	theme	cuttlefish	1413:1422	arg1	agents					1513:1518	antibacterial agents	1499:1518	antibacterial agents	1499:1518	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	4	12	theme	monosaccharide	748:761	arg1	composition					763:773	its monosaccharide composition	744:773	its monosaccharide composition	744:773	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	13	theme	galacturonic	655:666	arg1	acid					668:671	galacturonic acid	655:671	galacturonic acid (GalA)	655:678	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	13	theme	galacturonic	655:666	arg1	GalA					674:677	GalA	674:677	GalA	674:677	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	5	14	theme	important	823:831	arg1	activity					847:854	important antibacterial activity	823:854	important antibacterial activity	823:854	Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria.					
30465838	3	15	theme	spectroscopic	475:487	arg1	analysis					489:496	Infrared spectroscopic analysis	466:496	Infrared spectroscopic analysis	466:496	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	6	16	theme	in	977:978	arg1	tests					986:990	various in vitro tests	969:990	various in vitro tests	969:990	In addition, they exhibited strong antioxidant activities as showed by various in vitro tests.					
30465838	2	17	theme	sulfated	401:408	arg1	groups					410:415	sulfated groups	401:415	sulfated groups (6.6%)	401:422	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	17	theme	sulfated	401:408	arg1	%					421:421	6.6%	418:421	6.6%	418:421	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	4	18	theme	highest	718:724	arg1	GalA					736:739	GalA	736:739	GalA	736:739	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	18	theme	highest	718:724	arg1	amount					726:731	highest amount	718:731	highest amount of GalA	718:739	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	7	19	theme	elution	1097:1103	arg1	phase					1105:1109	the buffer elution phase	1086:1109	the buffer elution phase	1086:1109	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	8	20	dep	antioxidant	1332:1342	arg1	the					1323:1325	the	1323:1325	the	1323:1325	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	2	21	theme	acids	432:436	arg1	acids					432:436	uronic acids	425:436	uronic acids (9.2%)	425:443	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	proteins					449:456	proteins	449:456	proteins (3.7%)	449:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	groups					410:415	sulfated groups	401:415	sulfated groups (6.6%)	401:422	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	%					421:421	6.6%	418:421	6.6%	418:421	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	%					442:442	9.2%	439:442	9.2%	439:442	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	amounts					390:396	the highest amounts	378:396	the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%)	378:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	21	theme	acids	432:436	arg1	%					462:462	3.7%	459:462	3.7%	459:462	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	4	22	theme	GalA	736:739	arg1	GalA					736:739	GalA	736:739	GalA	736:739	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	22	theme	GalA	736:739	arg1	amount					726:731	highest amount	718:731	highest amount of GalA	718:739	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	3	23	theme	sulfonyl	524:531	arg1	OSO					534:536	sulfonyl (OSO) and acetyl (CH3CO-) groups	524:564	OSO	534:536	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	7	24	theme	buffer	1090:1095	arg1	phase					1105:1109	the buffer elution phase	1086:1109	the buffer elution phase	1086:1109	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	1	25	theme	cuttlefish	199:208	arg1	CSP					216:218	CSP	216:218	CSP	216:218	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	1	25	theme	cuttlefish	199:208	arg1	skin					210:213	cuttlefish skin	199:213	cuttlefish skin (CSP)	199:219	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	2	26	theme	uronic	425:430	arg1	acids					432:436	uronic acids	425:436	uronic acids (9.2%)	425:443	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	26	theme	uronic	425:430	arg1	%					442:442	9.2%	439:442	9.2%	439:442	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	9	27	theme	antibacterial	1499:1511	arg1	fractions					1450:1458	their fractions	1444:1458	their fractions	1444:1458	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	9	27	theme	antibacterial	1499:1511	arg1	polysaccharides					1424:1438	cuttlefish polysaccharides	1413:1438	cuttlefish polysaccharides	1413:1438	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	9	27	theme	antibacterial	1499:1511	arg1	agents					1513:1518	antibacterial agents	1499:1518	antibacterial agents	1499:1518	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	2	28	theme	highest	382:388	arg1	acids					432:436	uronic acids	425:436	uronic acids (9.2%)	425:443	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	proteins					449:456	proteins	449:456	proteins (3.7%)	449:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	groups					410:415	sulfated groups	401:415	sulfated groups (6.6%)	401:422	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	%					421:421	6.6%	418:421	6.6%	418:421	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	%					442:442	9.2%	439:442	9.2%	439:442	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	amounts					390:396	the highest amounts	378:396	the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%)	378:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	28	theme	highest	382:388	arg1	%					462:462	3.7%	459:462	3.7%	459:462	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	9	29	theme	obtained	1378:1385	arg1	results					1387:1393	The obtained results	1374:1393	The obtained results	1374:1393	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	8	30	theme	highest	1277:1283	arg1	charge					1294:1299	the highest negative charge	1273:1299	the highest negative charge	1273:1299	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	5	31	theme	Gram-	882:886	arg1	bacteria					888:895	several Gram+ and Gram- bacteria	864:895	bacteria	888:895	Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria.					
30465838	8	32	theme	eluting	1212:1218	arg1	fraction					1229:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	8	33	theme	best	1327:1330	arg1	antioxidant					1332:1342	antioxidant	1332:1342	antioxidant	1332:1342	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	6	34	theme	various	969:975	arg1	tests					986:990	various in vitro tests	969:990	various in vitro tests	969:990	In addition, they exhibited strong antioxidant activities as showed by various in vitro tests.					
30465838	0	35	theme	antioxidant	35:45	arg1	activities					65:74	antioxidant and antibacterial activities	35:74	antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle	35:144	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	8	36	theme	antibacterial	1348:1360	arg1	activities					1362:1371	antibacterial activities	1348:1371	antibacterial activities	1348:1371	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	3	37	theme	acetyl	543:548	arg1	groups					559:564	sulfonyl (OSO) and acetyl (CH3CO-) groups	524:564	groups	559:564	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	1	38	with	precipitation	242:254	arg1	cetylpyridinium					261:275	cetylpyridinium	261:275	cetylpyridinium	261:275	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	2	39	theme	groups	410:415	arg1	acids					432:436	uronic acids	425:436	uronic acids (9.2%)	425:443	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	proteins					449:456	proteins	449:456	proteins (3.7%)	449:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	groups					410:415	sulfated groups	401:415	sulfated groups (6.6%)	401:422	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	%					421:421	6.6%	418:421	6.6%	418:421	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	%					442:442	9.2%	439:442	9.2%	439:442	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	amounts					390:396	the highest amounts	378:396	the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%)	378:463	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	2	39	theme	groups	410:415	arg1	%					462:462	3.7%	459:462	3.7%	459:462	CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%).					
30465838	0	40	theme	activities	65:74	arg1	assessment					21:30	assessment	21:30	assessment	21:30	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	0	40	theme	activities	65:74	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	3	41	theme	OSO	534:536	arg1	presence					512:519	the presence	508:519	the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP	508:585	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	7	42	theme	linear	1178:1183	arg1	gradient					1185:1192	the linear gradient	1174:1192	the linear gradient of NaCl	1174:1200	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	0	43	theme	antibacterial	51:63	arg1	activities					65:74	antioxidant and antibacterial activities	35:74	antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle	35:144	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	1	44	theme	antioxidant	307:317	arg1	activities					337:346	their antioxidant and antibacterial activities	301:346	their antioxidant and antibacterial activities	301:346	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	7	45	theme	cuttlefish	1010:1019	arg1	polysaccharides					1021:1035	cuttlefish polysaccharides	1010:1035	cuttlefish polysaccharides	1010:1035	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	4	46	theme	major	683:687	arg1	components					689:698	major components	683:698	major components	683:698	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	46	theme	major	683:687	arg1	presence					616:623	the presence	612:623	the presence of glucuronic acid (GlcA) and galacturonic acid (GalA)	612:678	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	8	47	theme	negative	1285:1292	arg1	charge					1294:1299	the highest negative charge	1273:1299	the highest negative charge	1273:1299	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	4	48	theme	acid	639:642	arg1	components					689:698	major components	683:698	major components	683:698	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	48	theme	acid	639:642	arg1	presence					616:623	the presence	612:623	the presence of glucuronic acid (GlcA) and galacturonic acid (GalA)	612:678	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	8	49	from	sample	1248:1253	arg1	fraction					1229:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction	1203:1236	The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities.					
30465838	6	50	theme	antioxidant	933:943	arg1	activities					945:954	strong antioxidant activities	926:954	strong antioxidant activities	926:954	In addition, they exhibited strong antioxidant activities as showed by various in vitro tests.					
30465838	9	51	theme	natural	1475:1481	arg1	antioxidant					1483:1493	natural antioxidant	1475:1493	natural antioxidant	1475:1493	The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.					
30465838	3	52	theme	groups	559:564	arg1	presence					512:519	the presence	508:519	the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP	508:585	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	4	53	theme	glucuronic	628:637	arg1	GlcA					645:648	GlcA	645:648	GlcA	645:648	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	4	53	theme	glucuronic	628:637	arg1	acid					639:642	glucuronic acid	628:642	glucuronic acid (GlcA)	628:649	In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition.					
30465838	6	54	theme	strong	926:931	arg1	activities					945:954	strong antioxidant activities	926:954	strong antioxidant activities	926:954	In addition, they exhibited strong antioxidant activities as showed by various in vitro tests.					
30465838	0	55	theme	sulfated	79:86	arg1	polysaccharides					88:102	sulfated polysaccharides	79:102	sulfated polysaccharides extracted from cuttlefish skin and muscle	79:144	Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.					
30465838	7	56	theme	NaCl	1197:1200	arg1	gradient					1185:1192	the linear gradient	1174:1192	the linear gradient of NaCl	1174:1200	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
30465838	5	57	theme	Sulfated	776:783	arg1	polysaccharides					785:799	Sulfated polysaccharides	776:799	Sulfated polysaccharides	776:799	Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria.					
30465838	1	58	theme	antibacterial	323:335	arg1	activities					337:346	their antioxidant and antibacterial activities	301:346	their antioxidant and antibacterial activities	301:346	In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated.					
30465838	3	59	theme	CH3CO-	551:556	arg1	groups					559:564	sulfonyl (OSO) and acetyl (CH3CO-) groups	524:564	groups	559:564	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	3	60	theme	Infrared	466:473	arg1	analysis					489:496	Infrared spectroscopic analysis	466:496	Infrared spectroscopic analysis	466:496	Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP.					
30465838	7	61	theme	major	1121:1125	arg1	fractions					1127:1135	three major fractions	1115:1135	three major fractions for CMP	1115:1143	Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl.					
29520599	4	0	theme	EPS	486:488	arg1	yield					490:494	a relatively high EPS yield	468:494	a relatively high EPS yield	468:494	Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure.					
29520599	8	1	with	biolubricant	921:932	arg1	characteristics					939:953	characteristics	939:953	characteristics better than the conventional lubricant 'grease'	939:1001	Its potential as a biolubricant with characteristics better than the conventional lubricant 'grease' was revealed through analysis.					
29520599	8	1	with	biolubricant	921:932	arg1	grease					995:1000	the conventional lubricant 'grease'	967:1001	characteristics better than the conventional lubricant 'grease'	939:1001	Its potential as a biolubricant with characteristics better than the conventional lubricant 'grease' was revealed through analysis.					
29520599	0	2	from	potential	13:21	arg1	Cyanothece					69:78	the cyanobacterium Cyanothece	50:78	the cyanobacterium Cyanothece	50:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	4	3	theme	tested	399:404	arg1	stresses					406:413	the tested stresses	395:413	the tested stresses	395:413	Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure.					
29520599	7	4	theme	hydrophobic	829:839	arg1	flocculant					890:899	a flocculant	888:899	an excellent emulsifier as well as a flocculant	853:899	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	4	theme	hydrophobic	829:839	arg1	emulsifier					866:875	an excellent emulsifier	853:875	an excellent emulsifier as well as a flocculant	853:899	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	4	theme	hydrophobic	829:839	arg1	EPS					783:785	EPS	783:785	EPS from C. epiphytica	783:804	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	4	theme	hydrophobic	829:839	arg1	dispersant					841:850	a good hydrophobic dispersant	822:850	a good hydrophobic dispersant	822:850	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	3	5	from	effect	293:298	arg1	ability					331:337	the ability	327:337	the ability of Cyanothece epiphytica to produce EPS	327:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	0	6	from	Cyanothece	69:78	arg1	exopolysaccharides					26:43	exopolysaccharides	26:43	exopolysaccharides from the cyanobacterium Cyanothece	26:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	0	6	from	Cyanothece	69:78	arg1	potential					13:21	Biolubricant potential	0:21	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece	0:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	3	7	theme	epiphytica	353:362	arg1	ability					331:337	the ability	327:337	the ability of Cyanothece epiphytica to produce EPS	327:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	3	8	theme	nutrient	268:275	arg1	factors					277:283	nutrient factors	268:283	nutrient factors	268:283	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	7	9	theme	good	824:827	arg1	flocculant					890:899	a flocculant	888:899	an excellent emulsifier as well as a flocculant	853:899	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	9	theme	good	824:827	arg1	emulsifier					866:875	an excellent emulsifier	853:875	an excellent emulsifier as well as a flocculant	853:899	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	9	theme	good	824:827	arg1	EPS					783:785	EPS	783:785	EPS from C. epiphytica	783:804	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	9	theme	good	824:827	arg1	dispersant					841:850	a good hydrophobic dispersant	822:850	a good hydrophobic dispersant	822:850	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	1	10	theme	protective	178:187	arg1	layer					189:193	a protective layer	176:193	a protective layer termed sheath or capsule	176:218	Exopolysaccaharides (EPS) are carbohydrate polymers secreted by microbial cells, as a protective layer termed sheath or capsule.					
29520599	4	11	theme	high	481:484	arg1	yield					490:494	a relatively high EPS yield	468:494	a relatively high EPS yield	468:494	Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure.					
29520599	3	12	theme	factors	277:283	arg1	Optimisation					252:263	Optimisation	252:263	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS	252:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	6	13	theme	functional	764:773	arg1	groups					775:780	different functional groups	754:780	different functional groups	754:780	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	9	14	from	epiphytica	1201:1210	arg1	lubricant					1164:1172	lubricant	1164:1172	lubricant	1164:1172	This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.					
29520599	6	15	theme	different	754:762	arg1	groups					775:780	different functional groups	754:780	different functional groups	754:780	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	0	16	theme	Biolubricant	0:11	arg1	potential					13:21	Biolubricant potential	0:21	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece	0:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	3	17	from	Optimisation	252:263	arg1	ability					331:337	the ability	327:337	the ability of Cyanothece epiphytica to produce EPS	327:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	8	18	theme	lubricant	984:992	arg1	grease					995:1000	the conventional lubricant 'grease'	967:1001	characteristics better than the conventional lubricant 'grease'	939:1001	Its potential as a biolubricant with characteristics better than the conventional lubricant 'grease' was revealed through analysis.					
29520599	8	19	dep	better	955:960	arg1	than					962:965	than	962:965	than	962:965	Its potential as a biolubricant with characteristics better than the conventional lubricant 'grease' was revealed through analysis.					
29520599	0	20	theme	exopolysaccharides	26:43	arg1	potential					13:21	Biolubricant potential	0:21	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece	0:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	9	21	theme	cyanobacterium	1183:1196	arg1	epiphytica					1201:1210	the cyanobacterium C. epiphytica	1179:1210	the cyanobacterium C. epiphytica	1179:1210	This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.					
29520599	3	22	theme	effect	293:298	arg1	Optimisation					252:263	Optimisation	252:263	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS	252:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	8	23	theme	conventional	971:982	arg1	grease					995:1000	the conventional lubricant 'grease'	967:1001	characteristics better than the conventional lubricant 'grease'	939:1001	Its potential as a biolubricant with characteristics better than the conventional lubricant 'grease' was revealed through analysis.					
29520599	6	24	dep	hexoses	650:656	arg1	glucose					658:664	glucose	658:664	glucose	658:664	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	24	dep	hexoses	650:656	arg1	mannose					681:687	mannose	681:687	mannose	681:687	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	24	dep	hexoses	650:656	arg1	hexoses					650:656	hexoses glucose, galactose and mannose	650:687	hexoses glucose, galactose and mannose	650:687	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	24	dep	hexoses	650:656	arg1	galactose					667:675	galactose	667:675	galactose	667:675	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	25	dep	pentoses	619:626	arg1	arabinose					628:636	arabinose	628:636	arabinose	628:636	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	25	dep	pentoses	619:626	arg1	pentoses					619:626	pentoses arabinose and xylose	619:647	pentoses arabinose and xylose	619:647	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	25	dep	pentoses	619:626	arg1	xylose					642:647	xylose	642:647	xylose	642:647	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	4	26	from	0.06 mg/L	446:454	arg1	exposure					416:423	exposure	416:423	exposure to ozone for 50 s at 0.06 mg/L	416:454	Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure.					
29520599	1	27	theme	carbohydrate	122:133	arg1	Exopolysaccaharides					92:110	Exopolysaccaharides	92:110	Exopolysaccaharides (EPS)	92:116	Exopolysaccaharides (EPS) are carbohydrate polymers secreted by microbial cells, as a protective layer termed sheath or capsule.					
29520599	1	27	theme	carbohydrate	122:133	arg1	polymers					135:142	carbohydrate polymers	122:142	carbohydrate polymers secreted by microbial cells	122:170	Exopolysaccaharides (EPS) are carbohydrate polymers secreted by microbial cells, as a protective layer termed sheath or capsule.					
29520599	3	28	from	factors	277:283	arg1	ability					331:337	the ability	327:337	the ability of Cyanothece epiphytica to produce EPS	327:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	9	29	theme	tribological	1216:1227	arg1	applications					1229:1240	tribological applications	1216:1240	tribological applications	1216:1240	This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.					
29520599	7	30	from	epiphytica	795:804	arg1	EPS					783:785	EPS	783:785	EPS from C. epiphytica	783:804	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	30	from	epiphytica	795:804	arg1	dispersant					841:850	a good hydrophobic dispersant	822:850	a good hydrophobic dispersant	822:850	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	9	31	theme	commercial	1076:1085	arg1	technology					1087:1096	a commercial technology	1074:1096	a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications	1074:1240	This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.					
29520599	3	32	theme	stresses	315:322	arg1	effect					293:298	the effect	289:298	the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS	289:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	3	32	theme	stresses	315:322	arg1	factors					277:283	nutrient factors	268:283	nutrient factors	268:283	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	0	33	theme	cyanobacterium	54:67	arg1	Cyanothece					69:78	the cyanobacterium Cyanothece	50:78	the cyanobacterium Cyanothece	50:78	Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica.					
29520599	4	34	theme	cell	519:522	arg1	structure					524:532	cell structure	519:532	cell structure	519:532	Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure.					
29520599	3	35	from	ability	331:337	arg1	Optimisation					252:263	Optimisation	252:263	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS	252:377	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	3	36	theme	simple	308:313	arg1	stresses					315:322	some simple stresses	303:322	some simple stresses	303:322	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	6	37	theme	fucose	710:715	arg1	sugars					717:722	the deoxyhexose fucose sugars	694:722	the deoxyhexose fucose sugars which were sulphated and with different functional groups	694:780	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	37	theme	fucose	710:715	arg1	sulphated					735:743	sulphated	735:743	sulphated	735:743	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	1	38	theme	microbial	156:164	arg1	cells					166:170	microbial cells	156:170	microbial cells	156:170	Exopolysaccaharides (EPS) are carbohydrate polymers secreted by microbial cells, as a protective layer termed sheath or capsule.					
29520599	7	39	theme	excellent	856:864	arg1	emulsifier					866:875	an excellent emulsifier	853:875	an excellent emulsifier as well as a flocculant	853:899	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	7	39	theme	excellent	856:864	arg1	dispersant					841:850	a good hydrophobic dispersant	822:850	a good hydrophobic dispersant	822:850	EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant.					
29520599	6	40	theme	deoxyhexose	698:708	arg1	sugars					717:722	the deoxyhexose fucose sugars	694:722	the deoxyhexose fucose sugars which were sulphated and with different functional groups	694:780	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	6	40	theme	deoxyhexose	698:708	arg1	sulphated					735:743	sulphated	735:743	sulphated	735:743	Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups.					
29520599	3	41	theme	Cyanothece	342:351	arg1	epiphytica					353:362	Cyanothece epiphytica	342:362	Cyanothece epiphytica	342:362	Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested.					
29520599	9	42	dep	cyanobacterium	1183:1196	arg1	C.					1198:1199	C.	1198:1199	C.	1198:1199	This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.					
31398403	8	0	theme	feed	1449:1452	arg1	HP-02					1411:1415	HP-02	1411:1415	HP-02	1411:1415	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	8	0	theme	feed	1449:1452	arg1	supplement					1454:1463	a common carp feed supplement	1435:1463	a common carp feed supplement	1435:1463	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	0	1	theme	major	189:193	arg1	reservoirs					195:204	major reservoirs	189:204	major reservoirs of Aeromonas hydrophila	189:228	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	6	2	theme	cells	863:867	arg1	proliferation					817:829	proliferation	817:829	proliferation	817:829	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	2	theme	cells	863:867	arg1	phagocytosis					835:846	phagocytosis	835:846	phagocytosis	835:846	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	1	3	theme	polysaccharides	260:274	arg1	use					235:237	The use	231:237	The use of Chinese medicinal polysaccharides	231:274	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31398403	0	4	theme	immunoregulatory	76:91	arg1	effects					123:129	its immunoregulatory and anti-Aeromonas hydrophila effects	72:129	its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems	72:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	6	5	dep	proliferation	817:829	arg1	the					813:815	the	813:815	the	813:815	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	0	6	dep	Cyprinus	134:141	arg1	L.					150:151	Cyprinus carpio L.	134:151	Cyprinus carpio L. Aquaculture systems	134:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	8	7	theme	common	1368:1373	arg1	carp					1375:1378	common carp	1368:1378	common carp both in vitro and in vivo	1368:1404	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	5	8	from	galactose	723:731	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	0	9	theme	hydrophila	112:121	arg1	effects					123:129	its immunoregulatory and anti-Aeromonas hydrophila effects	72:129	its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems	72:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	10	from	Characterization	0:15	arg1	systems					165:171	Cyprinus carpio L. Aquaculture systems	134:171	Cyprinus carpio L. Aquaculture systems	134:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	10	from	Characterization	0:15	arg1	flowers					60:66	Honeysuckle flowers	48:66	Honeysuckle flowers	48:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	5	11	theme	Monosaccharide	632:645	arg1	composition					647:657	Monosaccharide composition	632:657	Monosaccharide composition	632:657	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	6	12	dep	cytokines	1043:1051	arg1	cytokines					1043:1051	the anti-inflammatory cytokines	1021:1051	the anti-inflammatory cytokines IL-10 and TGF-β	1021:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	12	dep	cytokines	1043:1051	arg1	TGF-β					1063:1067	TGF-β	1063:1067	TGF-β	1063:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	12	dep	cytokines	1043:1051	arg1	IL-10					1053:1057	IL-10	1053:1057	IL-10	1053:1057	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	5	13	from	glucose	710:716	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	0	14	theme	anti-Aeromonas	97:110	arg1	hydrophila					112:121	anti-Aeromonas hydrophila	97:121	anti-Aeromonas hydrophila	97:121	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	15	from	flowers	60:66	arg1	Characterization					0:15	Characterization	0:15	Characterization of a polysaccharide HP-02 from Honeysuckle flowers	0:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	15	from	flowers	60:66	arg1	effects					123:129	its immunoregulatory and anti-Aeromonas hydrophila effects	72:129	its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems	72:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	15	from	flowers	60:66	arg1	HP-02					37:41	a polysaccharide HP-02	20:41	a polysaccharide HP-02 from Honeysuckle flowers	20:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	1	16	from	diseases	330:337	arg1	aquaculture					342:352	aquaculture	342:352	aquaculture	342:352	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31398403	5	17	theme	molar	738:742	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	6	18	theme	kidney	856:861	arg1	cells					863:867	head kidney cells	851:867	head kidney cells	851:867	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	0	19	theme	hydrophila	219:228	arg1	reservoirs					195:204	major reservoirs	189:204	major reservoirs of Aeromonas hydrophila	189:228	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	8	20	theme	immunomodulatory	1340:1355	arg1	effects					1357:1363	immunomodulatory effects	1340:1363	immunomodulatory effects	1340:1363	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	6	21	theme	head	851:854	arg1	cells					863:867	head kidney cells	851:867	head kidney cells	851:867	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	0	22	theme	Aeromonas	209:217	arg1	hydrophila					219:228	Aeromonas hydrophila	209:228	Aeromonas hydrophila	209:228	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	8	23	contain	had	1336:1338	arg1	HP-02					1330:1334	HP-02	1330:1334	HP-02	1330:1334	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	8	23	contain	had	1336:1338	arg2	effects					1357:1363	immunomodulatory effects	1340:1363	immunomodulatory effects	1340:1363	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	2	24	theme	honeysuckle	412:422	arg1	flowers					424:430	honeysuckle flowers	412:430	honeysuckle flowers	412:430	A water-soluble polysaccharide, HP-02, was purified from honeysuckle flowers, and the present study was aimed to examine its immunomodulatory and anti-A.					
31398403	4	25	theme	HP-02	598:602	arg1	38 kDa					624:629	38 kDa	624:629	38 kDa	624:629	The results showed that the molecular weight of HP-02 was estimated to be 38 kDa.					
31398403	4	25	theme	HP-02	598:602	arg1	weight					588:593	the molecular weight	574:593	the molecular weight of HP-02	574:602	The results showed that the molecular weight of HP-02 was estimated to be 38 kDa.					
31398403	8	26	theme	fish	1476:1479	arg1	immunity					1481:1488	fish immunity	1476:1488	fish immunity	1476:1488	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	6	27	theme	anti-inflammatory	1025:1041	arg1	cytokines					1043:1051	the anti-inflammatory cytokines	1021:1051	the anti-inflammatory cytokines IL-10 and TGF-β	1021:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	27	theme	anti-inflammatory	1025:1041	arg1	TGF-β					1063:1067	TGF-β	1063:1067	TGF-β	1063:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	27	theme	anti-inflammatory	1025:1041	arg1	IL-10					1053:1057	IL-10	1053:1057	IL-10	1053:1057	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	5	28	theme	1.9	773:775	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	1	29	theme	successful	289:298	arg1	strategy					300:307	a successful strategy	287:307	a successful strategy to prevent bacterial diseases in aquaculture	287:352	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31398403	3	30	dep	Cyprinus	531:538	arg1	carpio					540:545	Cyprinus carpio L	531:547	Cyprinus carpio L	531:547	hydrophila effects in Cyprinus carpio L.					
31398403	3	30	dep	Cyprinus	531:538	arg1	L					547:547	L	547:547	Cyprinus carpio L	531:547	hydrophila effects in Cyprinus carpio L.					
31398403	0	31	theme	polysaccharide	22:35	arg1	HP-02					37:41	a polysaccharide HP-02	20:41	a polysaccharide HP-02 from Honeysuckle flowers	20:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	3	32	from	effects	520:526	arg1	Cyprinus					531:538	Cyprinus	531:538	Cyprinus	531:538	hydrophila effects in Cyprinus carpio L.					
31398403	2	33	theme	present	441:447	arg1	study					449:453	the present study	437:453	the present study	437:453	A water-soluble polysaccharide, HP-02, was purified from honeysuckle flowers, and the present study was aimed to examine its immunomodulatory and anti-A.					
31398403	0	34	theme	Cyprinus	134:141	arg1	systems					165:171	Cyprinus carpio L. Aquaculture systems	134:171	Cyprinus carpio L. Aquaculture systems	134:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	6	35	theme	kidney	1098:1103	arg1	cells					1105:1109	head kidney cells	1093:1109	head kidney cells	1093:1109	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	7	36	theme	cytokines	1290:1298	arg1	expression					1258:1267	the expression	1254:1267	the expression of anti-inflammatory cytokines	1254:1298	Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines.					
31398403	4	37	theme	molecular	578:586	arg1	38 kDa					624:629	38 kDa	624:629	38 kDa	624:629	The results showed that the molecular weight of HP-02 was estimated to be 38 kDa.					
31398403	4	37	theme	molecular	578:586	arg1	weight					588:593	the molecular weight	574:593	the molecular weight of HP-02	574:602	The results showed that the molecular weight of HP-02 was estimated to be 38 kDa.					
31398403	0	38	theme	Aquaculture	153:163	arg1	systems					165:171	Cyprinus carpio L. Aquaculture systems	134:171	Cyprinus carpio L. Aquaculture systems	134:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	39	theme	HP-02	37:41	arg1	Characterization					0:15	Characterization	0:15	Characterization of a polysaccharide HP-02 from Honeysuckle flowers	0:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	39	theme	HP-02	37:41	arg1	effects					123:129	its immunoregulatory and anti-Aeromonas hydrophila effects	72:129	its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems	72:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	1	40	theme	bacterial	320:328	arg1	diseases					330:337	bacterial diseases	320:337	bacterial diseases in aquaculture	320:352	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31398403	6	41	theme	cytokines	977:985	arg1	secretion					943:951	the secretion	939:951	the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β	939:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	5	42	from	rhamnose	691:698	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	6	43	from	level	1084:1088	arg1	serum					1115:1119	serum	1115:1119	serum	1115:1119	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	43	from	level	1084:1088	arg1	cells					1105:1109	head kidney cells	1093:1109	head kidney cells	1093:1109	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	5	44	from	arabinose	680:688	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	8	45	theme	disease	1494:1500	arg1	resistance					1502:1511	disease resistance	1494:1511	disease resistance	1494:1511	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	6	46	theme	head	1093:1096	arg1	cells					1105:1109	head kidney cells	1093:1109	head kidney cells	1093:1109	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	0	47	theme	Honeysuckle	48:58	arg1	flowers					60:66	Honeysuckle flowers	48:66	Honeysuckle flowers	48:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	6	48	theme	immunoregulation	899:914	arg1	activity					916:923	significant immunoregulation activity	887:923	significant immunoregulation activity	887:923	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	2	49	theme	water-soluble	357:369	arg1	HP-02					387:391	HP-02	387:391	HP-02	387:391	A water-soluble polysaccharide, HP-02, was purified from honeysuckle flowers, and the present study was aimed to examine its immunomodulatory and anti-A.					
31398403	2	49	theme	water-soluble	357:369	arg1	polysaccharide					371:384	A water-soluble polysaccharide	355:384	A water-soluble polysaccharide	355:384	A water-soluble polysaccharide, HP-02, was purified from honeysuckle flowers, and the present study was aimed to examine its immunomodulatory and anti-A.					
31398403	6	50	theme	significant	887:897	arg1	activity					916:923	significant immunoregulation activity	887:923	significant immunoregulation activity	887:923	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	0	51	from	effects	123:129	arg1	systems					165:171	Cyprinus carpio L. Aquaculture systems	134:171	Cyprinus carpio L. Aquaculture systems	134:171	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	0	51	from	effects	123:129	arg1	flowers					60:66	Honeysuckle flowers	48:66	Honeysuckle flowers	48:66	Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in Cyprinus carpio L. Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila.					
31398403	7	52	theme	cytokines	1230:1238	arg1	expression					1199:1208	the expression	1195:1208	the expression of pro-inflammatory cytokines	1195:1238	Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines.					
31398403	5	53	from	mannose	701:707	arg1	ratio					744:748	a molar ratio	736:748	a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9	736:775	Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9.					
31398403	8	54	theme	carp	1444:1447	arg1	HP-02					1411:1415	HP-02	1411:1415	HP-02	1411:1415	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	8	54	theme	carp	1444:1447	arg1	supplement					1454:1463	a common carp feed supplement	1435:1463	a common carp feed supplement	1435:1463	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	6	55	dep	cytokines	977:985	arg1	IL-12					1011:1015	IL-12	1011:1015	IL-12	1011:1015	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	55	dep	cytokines	977:985	arg1	IL-1β					994:998	IL-1β	994:998	IL-1β	994:998	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	55	dep	cytokines	977:985	arg1	TNF-α					987:991	TNF-α	987:991	TNF-α	987:991	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	55	dep	cytokines	977:985	arg1	cytokines					977:985	the pro-inflammatory cytokines	956:985	the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12	956:1015	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	55	dep	cytokines	977:985	arg1	IL-6					1001:1004	IL-6	1001:1004	IL-6	1001:1004	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	56	theme	protein	1076:1082	arg1	level					1084:1088	the protein level	1072:1088	the protein level in head kidney cells and serum	1072:1119	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	8	57	theme	common	1437:1442	arg1	HP-02					1411:1415	HP-02	1411:1415	HP-02	1411:1415	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	8	57	theme	common	1437:1442	arg1	supplement					1454:1463	a common carp feed supplement	1435:1463	a common carp feed supplement	1435:1463	These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31398403	7	58	theme	anti-inflammatory	1272:1288	arg1	cytokines					1290:1298	anti-inflammatory cytokines	1272:1298	anti-inflammatory cytokines	1272:1298	Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines.					
31398403	6	59	theme	cytokines	1043:1051	arg1	secretion					943:951	the secretion	939:951	the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β	939:1067	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	1	60	theme	Chinese	242:248	arg1	polysaccharides					260:274	Chinese medicinal polysaccharides	242:274	Chinese medicinal polysaccharides	242:274	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31398403	6	61	theme	pro-inflammatory	960:975	arg1	cytokines					977:985	the pro-inflammatory cytokines	956:985	the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12	956:1015	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	61	theme	pro-inflammatory	960:975	arg1	IL-12					1011:1015	IL-12	1011:1015	IL-12	1011:1015	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	61	theme	pro-inflammatory	960:975	arg1	TNF-α					987:991	TNF-α	987:991	TNF-α	987:991	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	61	theme	pro-inflammatory	960:975	arg1	IL-1β					994:998	IL-1β	994:998	IL-1β	994:998	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	6	61	theme	pro-inflammatory	960:975	arg1	IL-6					1001:1004	IL-6	1001:1004	IL-6	1001:1004	HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum.					
31398403	7	62	theme	pathogen	1139:1146	arg1	infection					1148:1156	pathogen infection	1139:1156	pathogen infection in vivo	1139:1164	Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines.					
31398403	7	63	theme	pro-inflammatory	1213:1228	arg1	cytokines					1230:1238	pro-inflammatory cytokines	1213:1238	pro-inflammatory cytokines	1213:1238	Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines.					
31398403	1	64	theme	medicinal	250:258	arg1	polysaccharides					260:274	Chinese medicinal polysaccharides	242:274	Chinese medicinal polysaccharides	242:274	The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture.					
31226937	3	0	theme	cell	290:293	arg1	wall					295:298	The plant cell wall	280:298	The plant cell wall	280:298	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	8	1	theme	pectin	1130:1135	arg1	methylation					1137:1147	the total pectin methylation	1120:1147	the total pectin methylation	1120:1147	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	5	2	theme	mass	611:614	arg1	spectrometry					616:627	mass spectrometry	611:627	mass spectrometry (LC-MS)	611:635	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	5	2	theme	mass	611:614	arg1	LC-MS					630:634	LC-MS	630:634	LC-MS	630:634	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	7	3	theme	increased	972:980	arg1	presence					982:989	an increased presence	969:989	an increased presence of xylogalacturonan in response to Cd	969:1027	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	1	4	theme	metal	148:152	arg1	Cd					163:164	Cd	163:164	Cd	163:164	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	1	4	theme	metal	148:152	arg1	cadmium					154:160	The heavy metal cadmium	138:160	The heavy metal cadmium (Cd)	138:165	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	7	5	theme	Structural	871:880	arg1	changes					882:888	Structural changes	871:888	Structural changes mainly appeared in the composition of pectic polysaccharides and data	871:958	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	4	6	theme	cell	467:470	arg1	composition					477:487	cell wall composition	467:487	cell wall composition	467:487	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	6	7	theme	Several	792:798	arg1	proteins					800:807	Several proteins	792:807	Several proteins determining for the cell wall structure changed in abundance	792:868	RESULTS Several proteins determining for the cell wall structure changed in abundance.					
31226937	9	8	theme	cell	1253:1256	arg1	wall					1258:1261	the cell wall	1249:1261	the cell wall due to the methylation of its galacturonic acid backbone	1249:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	10	9	from	wall	1365:1368	arg1	exclusion					1335:1343	the exclusion	1331:1343	the exclusion of Cd from the cell wall and apoplast	1331:1381	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	5	10	theme	targeted	693:700	arg1	analysis					718:725	targeted gene expression analysis	693:725	targeted gene expression analysis	693:725	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	9	11	theme	galacturonic	1293:1304	arg1	backbone					1311:1318	its galacturonic acid backbone	1289:1318	its galacturonic acid backbone	1289:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	10	12	theme	metal	1413:1417	arg1	entry					1394:1398	the entry	1390:1398	the entry of the heavy metal into the symplast	1390:1435	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	9	13	theme	increased	1182:1190	arg1	abundance					1192:1200	An increased abundance	1179:1200	CONCLUSIONS An increased abundance of xylogalacturonan	1167:1220	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	2	14	theme	life	268:271	arg1	forms					273:277	all life forms	264:277	all life forms	264:277	It is unessential and harmful to all life forms.					
31226937	10	15	theme	Cd	1348:1349	arg1	exclusion					1335:1343	the exclusion	1331:1343	the exclusion of Cd from the cell wall and apoplast	1331:1381	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	4	16	theme	long-term	552:560	arg1	exposure					562:569	long-term exposure	552:569	long-term exposure to Cd	552:575	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	3	17	theme	environmental	333:345	arg1	stress					347:352	environmental stress	333:352	environmental stress	333:352	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	4	18	theme	current	437:443	arg1	study					445:449	the current study	433:449	the current study	433:449	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	4	19	from	changes	452:458	arg1	structure					493:501	structure	493:501	structure	493:501	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	4	19	from	changes	452:458	arg1	composition					477:487	cell wall composition	467:487	cell wall composition	467:487	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	7	20	theme	polysaccharides	935:949	arg1	composition					913:923	the composition	909:923	the composition of pectic polysaccharides and data	909:958	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	1	21	dep	BACKGROUND	127:136	arg1	accumulates					167:177	accumulates	167:177	accumulates in the environment due to anthropogenic influences	167:228	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	8	22	theme	total	1124:1128	arg1	methylation					1137:1147	the total pectin methylation	1120:1147	the total pectin methylation	1120:1147	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	3	23	theme	physical	308:315	arg1	barrier					317:323	a physical barrier	306:323	a physical barrier against environmental stress	306:352	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	0	24	from	composition	46:56	arg1	wall					96:99	the cell wall	87:99	the cell wall of Medicago sativa stems	87:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	5	25	theme	Liquid	578:583	arg1	chromatography					585:598	Liquid chromatography	578:598	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis	578:669	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	5	26	theme	composition	771:781	arg1	analyses					745:752	analyses	745:752	analyses of the cell wall composition	745:781	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	5	27	theme	wall	766:769	arg1	composition					771:781	the cell wall composition	757:781	the cell wall composition	757:781	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	3	28	from	changes	358:364	arg1	structure					383:391	the cell wall structure	369:391	the cell wall structure	369:391	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	8	29	theme	higher	1041:1046	arg1	abundance					1048:1056	a higher abundance	1039:1056	a higher abundance	1039:1056	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	9	30	theme	xylogalacturonan	1205:1220	arg1	abundance					1192:1200	An increased abundance	1179:1200	CONCLUSIONS An increased abundance of xylogalacturonan	1167:1220	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	7	31	attach	presence	982:989	arg1	response					1014:1021	response	1014:1021	response to Cd	1014:1027	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	7	31	attach	presence	982:989	arg2	xylogalacturonan					994:1009	xylogalacturonan	994:1009	xylogalacturonan	994:1009	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	0	32	theme	Medicago	104:111	arg1	stems					120:124	Medicago sativa stems	104:124	Medicago sativa stems	104:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	0	33	theme	long-term	5:13	arg1	exposure					23:30	long-term cadmium exposure	5:30	long-term cadmium exposure	5:30	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	8	34	theme	enzymatic	1062:1070	arg1	activity					1072:1079	enzymatic activity	1062:1079	enzymatic activity	1062:1079	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	7	35	theme	pectic	928:933	arg1	polysaccharides					935:949	pectic polysaccharides	928:949	pectic polysaccharides	928:949	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	9	36	theme	acid	1306:1309	arg1	backbone					1311:1318	its galacturonic acid backbone	1289:1318	its galacturonic acid backbone	1289:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	9	37	theme	backbone	1311:1318	arg1	methylation					1274:1284	the methylation	1270:1284	the methylation of its galacturonic acid backbone	1270:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	7	38	from	presence	982:989	arg1	response					1014:1021	response	1014:1021	response to Cd	1014:1027	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	4	39	dep	composition	477:487	arg1	the					463:465	the	463:465	the	463:465	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	4	40	theme	Medicago	506:513	arg1	stems					522:526	Medicago sativa stems	506:526	Medicago sativa stems	506:526	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	10	41	theme	tolerance	1471:1479	arg1	acquisition					1481:1491	tolerance acquisition	1471:1491	tolerance acquisition	1471:1491	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	10	42	theme	important	1447:1455	arg1	factor					1457:1462	an important factor	1444:1462	an important factor	1444:1462	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	0	43	theme	stems	120:124	arg1	wall					96:99	the cell wall	87:99	the cell wall of Medicago sativa stems	87:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	9	44	theme	Cd	1235:1236	arg1	binding					1238:1244	Cd binding	1235:1244	Cd binding in the cell wall due to the methylation of its galacturonic acid backbone	1235:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	7	45	theme	data	955:958	arg1	composition					913:923	the composition	909:923	the composition of pectic polysaccharides and data	909:958	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	0	46	theme	cadmium	15:21	arg1	exposure					23:30	long-term cadmium exposure	5:30	long-term cadmium exposure	5:30	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	4	47	theme	stems	522:526	arg1	structure					493:501	structure	493:501	structure	493:501	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	4	47	theme	stems	522:526	arg1	composition					477:487	cell wall composition	467:487	cell wall composition	467:487	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	0	48	theme	sativa	113:118	arg1	stems					120:124	Medicago sativa stems	104:124	Medicago sativa stems	104:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	3	49	theme	wall	378:381	arg1	structure					383:391	the cell wall structure	369:391	the cell wall structure	369:391	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	4	50	theme	sativa	515:520	arg1	stems					522:526	Medicago sativa stems	506:526	Medicago sativa stems	506:526	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	8	51	theme	methylesterase	1091:1104	arg1	abundance					1048:1056	a higher abundance	1039:1056	a higher abundance	1039:1056	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	8	51	theme	methylesterase	1091:1104	arg1	activity					1072:1079	enzymatic activity	1062:1079	enzymatic activity	1062:1079	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	3	52	theme	Cd	417:418	arg1	exposure					420:427	Cd exposure	417:427	Cd exposure	417:427	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	8	53	theme	pectin	1084:1089	arg1	methylesterase					1091:1104	pectin methylesterase	1084:1104	pectin methylesterase	1084:1104	Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.					
31226937	5	54	theme	gene	702:705	arg1	analysis					718:725	targeted gene expression analysis	693:725	targeted gene expression analysis	693:725	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	1	55	theme	due	198:200	arg1	environment					186:196	the environment	182:196	the environment due to anthropogenic influences	182:228	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	5	56	theme	cell	761:764	arg1	composition					771:781	the cell wall composition	757:781	the cell wall composition	757:781	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	4	57	theme	wall	472:475	arg1	composition					477:487	cell wall composition	467:487	cell wall composition	467:487	In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd.					
31226937	6	58	theme	cell	829:832	arg1	structure					839:847	the cell wall structure	825:847	the cell wall structure changed in abundance	825:868	RESULTS Several proteins determining for the cell wall structure changed in abundance.					
31226937	3	59	theme	cell	373:376	arg1	structure					383:391	the cell wall structure	369:391	the cell wall structure	369:391	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	3	60	theme	plant	284:288	arg1	wall					295:298	The plant cell wall	280:298	The plant cell wall	280:298	The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure.					
31226937	7	61	theme	xylogalacturonan	994:1009	arg1	presence					982:989	an increased presence	969:989	an increased presence of xylogalacturonan in response to Cd	969:1027	Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd.					
31226937	6	62	theme	wall	834:837	arg1	structure					839:847	the cell wall structure	825:847	the cell wall structure changed in abundance	825:868	RESULTS Several proteins determining for the cell wall structure changed in abundance.					
31226937	5	63	theme	quantitative	641:652	arg1	analysis					662:669	quantitative protein analysis	641:669	quantitative protein analysis	641:669	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	5	64	theme	expression	707:716	arg1	analysis					718:725	targeted gene expression analysis	693:725	targeted gene expression analysis	693:725	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	5	65	theme	protein	654:660	arg1	analysis					662:669	quantitative protein analysis	641:669	quantitative protein analysis	641:669	Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.					
31226937	10	66	from	apoplast	1374:1381	arg1	exclusion					1335:1343	the exclusion	1331:1343	the exclusion of Cd from the cell wall and apoplast	1331:1381	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	10	67	theme	cell	1360:1363	arg1	wall					1365:1368	the cell wall	1356:1368	the cell wall	1356:1368	Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.					
31226937	9	68	dep	CONCLUSIONS	1167:1177	arg1	abundance					1192:1200	An increased abundance	1179:1200	CONCLUSIONS An increased abundance of xylogalacturonan	1167:1220	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	1	69	theme	anthropogenic	205:217	arg1	influences					219:228	anthropogenic influences	205:228	anthropogenic influences	205:228	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	0	70	theme	polysaccharides	68:82	arg1	composition					46:56	the composition	42:56	the composition of pectic polysaccharides in the cell wall of Medicago sativa stems	42:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	0	71	theme	pectic	61:66	arg1	polysaccharides					68:82	pectic polysaccharides	61:82	pectic polysaccharides	61:82	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31226937	1	72	theme	heavy	142:146	arg1	Cd					163:164	Cd	163:164	Cd	163:164	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	1	72	theme	heavy	142:146	arg1	cadmium					154:160	The heavy metal cadmium	138:160	The heavy metal cadmium (Cd)	138:165	BACKGROUND The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences.					
31226937	9	73	from	binding	1238:1244	arg1	wall					1258:1261	the cell wall	1249:1261	the cell wall due to the methylation of its galacturonic acid backbone	1249:1318	CONCLUSIONS An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone.					
31226937	0	74	theme	cell	91:94	arg1	wall					96:99	the cell wall	87:99	the cell wall of Medicago sativa stems	87:124	Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?					
31247951	4	0	theme	comprehensive	498:510	arg1	determination					512:524	the comprehensive determination	494:524	the comprehensive determination of blood circulating proteins and their glycans	494:572	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	3	1	from	variation	367:375	arg1	cohorts					426:432	cohorts	426:432	cohorts	426:432	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	3	1	from	variation	367:375	arg1	samples					414:420	experimentally well characterized samples	380:420	experimentally well characterized samples	380:420	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	6	2	with	structures	1095:1104	arg1	levels					1134:1139	immunoglobulin G (IgG) levels	1111:1139	immunoglobulin G (IgG) levels	1111:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	4	3	theme	blood	529:533	arg1	proteins					547:554	blood circulating proteins	529:554	blood circulating proteins	529:554	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	6	4	gly	fucosylated	1083:1093	arg1	structures					1095:1104	core fucosylated structures	1078:1104	core fucosylated structures with immunoglobulin G (IgG) levels	1078:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	5	5	from	individuals	873:883	arg1	samples					860:866	344 samples	856:866	344 samples from individuals of Arab, South-Asian, and Filipino descent	856:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	2	6	attach	Attachment	106:115	arg1	part					164:167	the polypeptide part	148:167	the polypeptide part of these proteins	148:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	6	attach	Attachment	106:115	arg2	oligosaccharides					128:143	complex oligosaccharides	120:143	complex oligosaccharides	120:143	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	4	7	theme	capable	451:457	arg1	methods					471:477	High-throughput capable large-scale methods	435:477	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans	435:572	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	5	8	theme	blood	707:711	arg1	proteome					720:727	the blood plasma proteome	703:727	the blood plasma proteome	703:727	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	2	9	theme	many	267:270	arg1	disorders					280:288	many complex disorders	267:288	many complex disorders	267:288	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	7	10	from	disorders	1409:1417	arg1	investigation					1344:1356	the investigation	1340:1356	the investigation of its role and function in the associated complex disorders	1340:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	4	11	dep	capable	451:457	arg1	large-scale					459:469	large-scale	459:469	large-scale	459:469	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	5	12	theme	plasma	713:718	arg1	proteome					720:727	the blood plasma proteome	703:727	the blood plasma proteome	703:727	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	2	13	theme	polypeptide	152:162	arg1	part					164:167	the polypeptide part	148:167	the polypeptide part of these proteins	148:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	7	14	theme	associated	1390:1399	arg1	disorders					1409:1417	the associated complex disorders	1386:1417	the associated complex disorders	1386:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	7	15	theme	function	1374:1381	arg1	investigation					1344:1356	the investigation	1340:1356	the investigation of its role and function in the associated complex disorders	1340:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	3	16	theme	human	329:333	arg1	glycan					335:340	this human glycan	324:340	this human glycan	324:340	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	6	17	theme	G	1126:1126	arg1	levels					1134:1139	immunoglobulin G (IgG) levels	1111:1139	immunoglobulin G (IgG) levels	1111:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	7	18	theme	N-glycosylation	1295:1309	arg1	picture					1276:1282	a detailed picture	1265:1282	a detailed picture of protein N-glycosylation	1265:1309	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	1	19	theme	Most	68:71	arg1	proteins					79:86	Most human proteins	68:86	Most human proteins	68:86	Most human proteins are glycosylated.					
31247951	1	20	gly	glycosylated	92:103	arg1	proteins					79:86	Most human proteins	68:86	Most human proteins	68:86	Most human proteins are glycosylated.					
31247951	3	21	theme	natural	359:365	arg1	variation					367:375	natural variation	359:375	natural variation in experimentally well characterized samples and cohorts	359:432	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	2	22	theme	oligosaccharides	128:143	arg1	Attachment					106:115	Attachment	106:115	Attachment of complex oligosaccharides to the polypeptide part of these proteins	106:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	22	theme	oligosaccharides	128:143	arg1	part					202:205	an integral part	190:205	an integral part of their structure and function	190:237	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	1	23	theme	human	73:77	arg1	proteins					79:86	Most human proteins	68:86	Most human proteins	68:86	Most human proteins are glycosylated.					
31247951	5	24	theme	blood	789:793	arg1	proteins					807:814	1116 blood circulating proteins	784:814	1116 blood circulating proteins	784:814	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	7	25	theme	complex	1401:1407	arg1	disorders					1409:1417	the associated complex disorders	1386:1417	the associated complex disorders	1386:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	5	26	theme	Filipino	911:918	arg1	descent					920:926	Filipino descent	911:926	Filipino descent	911:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	5	27	theme	344	856:858	arg1	samples					860:866	344 samples	856:866	344 samples from individuals of Arab, South-Asian, and Filipino descent	856:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	0	28	theme	Human	20:24	arg1	N-Glycome					39:47	the Human Blood Plasma N-Glycome	16:47	the Human Blood Plasma N-Glycome	16:47	Fine-Mapping of the Human Blood Plasma N-Glycome onto Its Proteome.					
31247951	4	29	theme	glycans	566:572	arg1	determination					512:524	the comprehensive determination	494:524	the comprehensive determination of blood circulating proteins and their glycans	494:572	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	5	30	theme	descent	920:926	arg1	individuals					873:883	individuals	873:883	individuals of Arab, South-Asian, and Filipino descent	873:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	7	31	from	investigation	1344:1356	arg1	disorders					1409:1417	the associated complex disorders	1386:1417	the associated complex disorders	1386:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	6	32	theme	N-glycosylation	1025:1039	arg1	correlation					1063:1073	a correlation	1061:1073	a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1061:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	32	theme	N-glycosylation	1025:1039	arg1	patterns					1041:1048	protein-specific N-glycosylation patterns	1008:1048	protein-specific N-glycosylation patterns	1008:1048	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	5	33	theme	proteins	807:814	arg1	levels					774:779	the levels	770:779	the levels of 1116 blood circulating proteins	770:814	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	5	34	theme	first	692:696	arg1	time					698:701	the first time	688:701	the first time	688:701	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	2	35	theme	proteins	178:185	arg1	part					164:167	the polypeptide part	148:167	the polypeptide part of these proteins	148:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	36	theme	complex	272:278	arg1	disorders					280:288	many complex disorders	267:288	many complex disorders	267:288	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	6	37	theme	protein-specific	1008:1023	arg1	correlation					1063:1073	a correlation	1061:1073	a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1061:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	37	theme	protein-specific	1008:1023	arg1	patterns					1041:1048	protein-specific N-glycosylation patterns	1008:1048	protein-specific N-glycosylation patterns	1008:1048	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	0	38	theme	Plasma	32:37	arg1	N-Glycome					39:47	the Human Blood Plasma N-Glycome	16:47	the Human Blood Plasma N-Glycome	16:47	Fine-Mapping of the Human Blood Plasma N-Glycome onto Its Proteome.					
31247951	4	39	theme	High-throughput	435:449	arg1	methods					471:477	High-throughput capable large-scale methods	435:477	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans	435:572	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	6	40	theme	heparin	1216:1222	arg1	cofactor					1224:1231	heparin cofactor 2	1216:1233	heparin cofactor 2 (SERPIND2)	1216:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	40	theme	heparin	1216:1222	arg1	SERPIND2					1236:1243	SERPIND2	1236:1243	SERPIND2	1236:1243	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	7	41	theme	protein	1287:1293	arg1	N-glycosylation					1295:1309	protein N-glycosylation	1287:1309	protein N-glycosylation	1287:1309	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	5	42	theme	N-glycan	825:832	arg1	traits					834:839	113 N-glycan traits	821:839	113 N-glycan traits	821:839	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	0	43	theme	Blood	26:30	arg1	N-Glycome					39:47	the Human Blood Plasma N-Glycome	16:47	the Human Blood Plasma N-Glycome	16:47	Fine-Mapping of the Human Blood Plasma N-Glycome onto Its Proteome.					
31247951	4	44	theme	proteins	547:554	arg1	determination					512:524	the comprehensive determination	494:524	the comprehensive determination of blood circulating proteins and their glycans	494:572	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	3	45	theme	characterized	400:412	arg1	samples					414:420	experimentally well characterized samples	380:420	experimentally well characterized samples	380:420	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	2	46	theme	structure	216:224	arg1	Attachment					106:115	Attachment	106:115	Attachment of complex oligosaccharides to the polypeptide part of these proteins	106:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	46	theme	structure	216:224	arg1	part					202:205	an integral part	190:205	an integral part of their structure and function	190:237	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	5	47	theme	circulating	795:805	arg1	proteins					807:814	1116 blood circulating proteins	784:814	1116 blood circulating proteins	784:814	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	4	48	theme	circulating	535:545	arg1	proteins					547:554	blood circulating proteins	529:554	blood circulating proteins	529:554	High-throughput capable large-scale methods that allow for the comprehensive determination of blood circulating proteins and their glycans have been recently developed, but so far, no study has investigated the link between both traits.					
31247951	6	49	theme	structures	1200:1209	arg1	correlation					1063:1073	a correlation	1061:1073	a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1061:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	5	50	theme	Arab	888:891	arg1	individuals					873:883	individuals	873:883	individuals of Arab, South-Asian, and Filipino descent	873:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	0	51	theme	N-Glycome	39:47	arg1	Fine-Mapping					0:11	Fine-Mapping	0:11	Fine-Mapping of the Human Blood Plasma N-Glycome onto Its Proteome.	0:66	Fine-Mapping of the Human Blood Plasma N-Glycome onto Its Proteome.					
31247951	6	52	theme	triantennary	1187:1198	arg1	structures					1200:1209	trisialylated, trigalactosylated, and triantennary structures	1149:1209	trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1149:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	53	theme	immunoglobulin	1111:1124	arg1	IgG					1129:1131	IgG	1129:1131	IgG	1129:1131	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	53	theme	immunoglobulin	1111:1124	arg1	G					1126:1126	immunoglobulin G	1111:1126	immunoglobulin G (IgG) levels	1111:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	7	54	from	role	1365:1368	arg1	disorders					1409:1417	the associated complex disorders	1386:1417	the associated complex disorders	1386:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	7	55	theme	new	1324:1326	arg1	avenues					1328:1334	new avenues	1324:1334	new avenues for the investigation of its role and function in the associated complex disorders	1324:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	3	56	dep	code	342:345	arg1	is					347:348	is	347:348	code is to study natural variation in experimentally well characterized samples and cohorts	342:432	One approach towards deciphering this human glycan code is to study natural variation in experimentally well characterized samples and cohorts.					
31247951	2	57	theme	integral	193:200	arg1	Attachment					106:115	Attachment	106:115	Attachment of complex oligosaccharides to the polypeptide part of these proteins	106:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	57	theme	integral	193:200	arg1	part					202:205	an integral part	190:205	an integral part of their structure and function	190:237	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	5	58	theme	European	979:986	arg1	subjects					967:974	46 subjects	964:974	46 subjects of European ancestry	964:995	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	5	59	theme	South-Asian	894:904	arg1	individuals					873:883	individuals	873:883	individuals of Arab, South-Asian, and Filipino descent	873:926	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	7	60	theme	role	1365:1368	arg1	investigation					1344:1356	the investigation	1340:1356	the investigation of its role and function in the associated complex disorders	1340:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	6	61	with	structures	1200:1209	arg1	cofactor					1224:1231	heparin cofactor 2	1216:1233	heparin cofactor 2 (SERPIND2)	1216:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	61	with	structures	1200:1209	arg1	SERPIND2					1236:1243	SERPIND2	1236:1243	SERPIND2	1236:1243	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	2	62	theme	central	251:257	arg1	role					259:262	a central role	249:262	a central role	249:262	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	6	63	theme	trisialylated	1149:1161	arg1	structures					1200:1209	trisialylated, trigalactosylated, and triantennary structures	1149:1209	trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1149:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	64	gly	trisialylated	1149:1161	arg1	structures					1200:1209	trisialylated, trigalactosylated, and triantennary structures	1149:1209	trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1149:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	6	65	theme	structures	1095:1104	arg1	correlation					1063:1073	a correlation	1061:1073	a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1061:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	7	66	from	function	1374:1381	arg1	disorders					1409:1417	the associated complex disorders	1386:1417	the associated complex disorders	1386:1417	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	6	67	theme	trigalactosylated	1164:1180	arg1	structures					1200:1209	trisialylated, trigalactosylated, and triantennary structures	1149:1209	trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2)	1149:1244	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	7	68	theme	detailed	1267:1274	arg1	picture					1276:1282	a detailed picture	1265:1282	a detailed picture of protein N-glycosylation	1265:1309	Our study reveals a detailed picture of protein N-glycosylation and suggests new avenues for the investigation of its role and function in the associated complex disorders.					
31247951	6	69	theme	fucosylated	1083:1093	arg1	structures					1095:1104	core fucosylated structures	1078:1104	core fucosylated structures with immunoglobulin G (IgG) levels	1078:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31247951	5	70	theme	matching	736:743	arg1	N-glycome					745:753	its matching N-glycome	732:753	its matching N-glycome	732:753	Here we map for the first time the blood plasma proteome to its matching N-glycome by correlating the levels of 1116 blood circulating proteins with 113 N-glycan traits, determined in 344 samples from individuals of Arab, South-Asian, and Filipino descent, and then replicate our findings in 46 subjects of European ancestry.					
31247951	2	71	theme	complex	120:126	arg1	oligosaccharides					128:143	complex oligosaccharides	120:143	complex oligosaccharides	120:143	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	72	theme	function	230:237	arg1	Attachment					106:115	Attachment	106:115	Attachment of complex oligosaccharides to the polypeptide part of these proteins	106:185	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	2	72	theme	function	230:237	arg1	part					202:205	an integral part	190:205	an integral part of their structure and function	190:237	Attachment of complex oligosaccharides to the polypeptide part of these proteins is an integral part of their structure and function and plays a central role in many complex disorders.					
31247951	6	73	theme	core	1078:1081	arg1	structures					1095:1104	core fucosylated structures	1078:1104	core fucosylated structures with immunoglobulin G (IgG) levels	1078:1139	We report protein-specific N-glycosylation patterns, including a correlation of core fucosylated structures with immunoglobulin G (IgG) levels, and of trisialylated, trigalactosylated, and triantennary structures with heparin cofactor 2 (SERPIND2).					
31601012	6	0	theme	lung	844:847	arg1	cancer					849:854	lung cancer	844:854	lung cancer	844:854	Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model.					
31601012	5	1	theme	NMR	632:634	arg1	analyses					646:653	Methylation, NMR and FT-IR analyses	619:653	analyses	646:653	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	2	2	theme	medicine	316:323	arg1	value					325:329	the medicine value	312:329	the medicine value of radix herb residues	312:352	To escape from this, the medicine value of radix herb residues is mined in this article.					
31601012	9	3	theme	subsets	1333:1339	arg1	index					1290:1294	the immune organ index	1273:1294	the immune organ index	1273:1294	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	9	3	theme	subsets	1333:1339	arg1	percentage					1301:1310	the percentage	1297:1310	the percentage of spleen lymphocyte subsets	1297:1339	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	9	3	theme	subsets	1333:1339	arg1	levels					1360:1365	serum cytokine levels	1345:1365	serum cytokine levels	1345:1365	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	0	4	theme	Polysaccharide	86:99	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	4	theme	Polysaccharide	86:99	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	4	theme	Polysaccharide	86:99	arg1	Antitumor					30:38	Antitumor	30:38	Antitumor	30:38	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	4	theme	Polysaccharide	86:99	arg1	Activities					58:67	Immunological Activities	44:67	Immunological Activities	44:67	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	9	5	theme	cancer	1375:1380	arg1	mice					1382:1385	lung cancer mice	1370:1385	lung cancer mice	1370:1385	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	1	6	theme	radix	248:252	arg1	radix					248:252	radix	248:252	radix	248:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	6	theme	radix	248:252	arg1	waste					271:275	a waste	269:275	a waste of resources	269:288	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	6	theme	radix	248:252	arg1	residues					236:243	the herb residues	227:243	the herb residues of radix	227:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	4	7	theme	Monosaccharide-composition	480:505	arg1	analysis					507:514	Monosaccharide-composition analysis	480:514	Monosaccharide-composition analysis	480:514	Monosaccharide-composition analysis revealed that AX-I-3b consisted of arabinose, xylose, and glucose with a molar ratio of 10.4:79.3:1.1.					
31601012	5	8	theme	FT-IR	640:644	arg1	analyses					646:653	Methylation, NMR and FT-IR analyses	619:653	analyses	646:653	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	6	9	theme	tumor	906:910	arg1	model					912:916	xenograft tumor model	896:916	xenograft tumor model	896:916	Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model.					
31601012	7	10	theme	Bcl-2	1107:1111	arg1	up-regulation					1031:1043	the up-regulation	1027:1043	the up-regulation of p53, Bax and cleaved caspase-3	1027:1077	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	7	10	theme	Bcl-2	1107:1111	arg1	down-regulation					1088:1102	the down-regulation	1084:1102	the down-regulation of Bcl-2	1084:1111	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	6	11	theme	antitumor	817:825	arg1	activity					827:834	antitumor activity	817:834	antitumor activity against lung cancer	817:854	Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model.					
31601012	10	12	theme	new	1548:1550	arg1	insight					1552:1558	a new insight	1546:1558	a new insight into the reutilization of radix herb residue	1546:1603	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	8	13	theme	xenograft	1197:1205	arg1	model					1214:1218	xenograft tumors model	1197:1218	xenograft tumors model	1197:1218	Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model.					
31601012	6	14	theme	xenograft	896:904	arg1	model					912:916	xenograft tumor model	896:916	xenograft tumor model	896:916	Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model.					
31601012	4	15	theme	10.4:79.3:1.1	604:616	arg1	ratio					595:599	a molar ratio	587:599	a molar ratio of 10.4:79.3:1.1	587:616	Monosaccharide-composition analysis revealed that AX-I-3b consisted of arabinose, xylose, and glucose with a molar ratio of 10.4:79.3:1.1.					
31601012	9	16	theme	cytokine	1351:1358	arg1	levels					1360:1365	serum cytokine levels	1345:1365	serum cytokine levels	1345:1365	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	8	17	theme	therapeutic	1161:1171	arg1	ability					1173:1179	the therapeutic ability	1157:1179	the therapeutic ability of cisplatin	1157:1192	Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model.					
31601012	10	18	theme	radix	1586:1590	arg1	residue					1597:1603	radix herb residue	1586:1603	radix herb residue	1586:1603	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	0	19	theme	Herb	123:126	arg1	Residue					128:134	Herb Residue	123:134	Herb Residue	123:134	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	9	20	theme	AX-I-3b	1234:1240	arg1	treatment					1242:1250	AX-I-3b treatment	1234:1250	AX-I-3b treatment	1234:1250	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	2	21	theme	residues	345:352	arg1	value					325:329	the medicine value	312:329	the medicine value of radix herb residues	312:352	To escape from this, the medicine value of radix herb residues is mined in this article.					
31601012	9	22	theme	immune	1277:1282	arg1	index					1290:1294	the immune organ index	1273:1294	the immune organ index	1273:1294	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	5	23	theme	Methylation	619:629	arg1	analyses					646:653	Methylation, NMR and FT-IR analyses	619:653	analyses	646:653	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	7	24	dep	induced	944:950	arg1	evidenced					1014:1022	evidenced	1014:1022	is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2	1011:1111	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	2	25	theme	herb	340:343	arg1	residues					345:352	radix herb residues	334:352	radix herb residues	334:352	To escape from this, the medicine value of radix herb residues is mined in this article.					
31601012	2	26	theme	radix	334:338	arg1	residues					345:352	radix herb residues	334:352	radix herb residues	334:352	To escape from this, the medicine value of radix herb residues is mined in this article.					
31601012	7	27	theme	cleaved	1061:1067	arg1	caspase-3					1069:1077	cleaved caspase-3	1061:1077	cleaved caspase-3	1061:1077	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	7	28	theme	xenograft	987:995	arg1	tumors					997:1002	xenograft tumors	987:1002	xenograft tumors	987:1002	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	10	29	theme	herb	1592:1595	arg1	residue					1597:1603	radix herb residue	1586:1603	radix herb residue	1586:1603	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	7	30	theme	caspase-3	1069:1077	arg1	up-regulation					1031:1043	the up-regulation	1027:1043	the up-regulation of p53, Bax and cleaved caspase-3	1027:1077	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	7	30	theme	caspase-3	1069:1077	arg1	down-regulation					1088:1102	the down-regulation	1084:1102	the down-regulation of Bcl-2	1084:1111	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	6	31	theme	MTT	882:884	arg1	assay					886:890	MTT assay	882:890	MTT assay	882:890	Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model.					
31601012	3	32	theme	radix	434:438	arg1	residues					445:452	radix herb residues	434:452	radix herb residues	434:452	We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction.					
31601012	7	33	theme	Bax	1053:1055	arg1	up-regulation					1031:1043	the up-regulation	1027:1043	the up-regulation of p53, Bax and cleaved caspase-3	1027:1077	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	7	33	theme	Bax	1053:1055	arg1	down-regulation					1088:1102	the down-regulation	1084:1102	the down-regulation of Bcl-2	1084:1111	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	3	34	theme	fractional	457:466	arg1	extraction					468:477	fractional extraction	457:477	fractional extraction	457:477	We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction.					
31601012	10	35	theme	residue	1597:1603	arg1	reutilization					1569:1581	the reutilization	1565:1581	the reutilization of radix herb residue	1565:1603	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	1	36	theme	clinical	192:199	arg1	application					201:211	clinical application	192:211	clinical application in China	192:220	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	3	37	theme	polysaccharide	406:419	arg1	AX-I-3b					421:427	hemicellulose polysaccharide AX-I-3b	392:427	hemicellulose polysaccharide AX-I-3b	392:427	We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction.					
31601012	9	38	theme	organ	1284:1288	arg1	index					1290:1294	the immune organ index	1273:1294	the immune organ index	1273:1294	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	9	39	theme	spleen	1315:1320	arg1	subsets					1333:1339	spleen lymphocyte subsets	1315:1339	spleen lymphocyte subsets	1315:1339	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	8	40	theme	cisplatin	1184:1192	arg1	ability					1173:1179	the therapeutic ability	1157:1179	the therapeutic ability of cisplatin	1157:1192	Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model.					
31601012	7	41	theme	p53	1048:1050	arg1	up-regulation					1031:1043	the up-regulation	1027:1043	the up-regulation of p53, Bax and cleaved caspase-3	1027:1077	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	7	41	theme	p53	1048:1050	arg1	down-regulation					1088:1102	the down-regulation	1084:1102	the down-regulation of Bcl-2	1084:1111	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	1	42	theme	resources	280:288	arg1	radix					248:252	radix	248:252	radix	248:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	42	theme	resources	280:288	arg1	waste					271:275	a waste	269:275	a waste of resources	269:288	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	42	theme	resources	280:288	arg1	residues					236:243	the herb residues	227:243	the herb residues of radix	227:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	7	43	theme	lung	965:968	arg1	cells					977:981	lung cancer cells	965:981	lung cancer cells	965:981	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	5	44	dep	follows	712:718	arg1	1→					775:776	1→	775:776	1→	775:776	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	8	45	theme	tumors	1207:1212	arg1	model					1214:1218	xenograft tumors model	1197:1218	xenograft tumors model	1197:1218	Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model.					
31601012	0	46	theme	Immunological	44:56	arg1	Activities					58:67	Immunological Activities	44:67	Immunological Activities	44:67	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	7	47	theme	cancer	970:975	arg1	cells					977:981	lung cancer cells	965:981	lung cancer cells	965:981	Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2.					
31601012	1	48	dep	turn	254:257	arg1	out					259:261	out	259:261	out	259:261	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	49	from	application	201:211	arg1	China					216:220	China	216:220	China	216:220	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	50	theme	Astragalus	137:146	arg1	radix					148:152	Astragalus radix	137:152	Astragalus radix (radix)	137:160	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	50	theme	Astragalus	137:146	arg1	radix					155:159	radix	155:159	radix	155:159	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	5	51	theme	AX-I-3b	667:673	arg1	follows					712:718	follows	712:718	follows	712:718	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	5	51	theme	AX-I-3b	667:673	arg1	residue					690:696	AX-I-3b monosaccharide residue	667:696	AX-I-3b monosaccharide residue	667:696	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	10	52	theme	antitumor	1498:1506	arg1	AX-I-3b					1484:1490	AX-I-3b	1484:1490	AX-I-3b	1484:1490	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	10	52	theme	antitumor	1498:1506	arg1	agent					1529:1533	an antitumor and immunomodulatory agent	1495:1533	an antitumor and immunomodulatory agent	1495:1533	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	3	53	theme	herb	440:443	arg1	residues					445:452	radix herb residues	434:452	radix herb residues	434:452	We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction.					
31601012	4	54	theme	molar	589:593	arg1	ratio					595:599	a molar ratio	587:599	a molar ratio of 10.4:79.3:1.1	587:616	Monosaccharide-composition analysis revealed that AX-I-3b consisted of arabinose, xylose, and glucose with a molar ratio of 10.4:79.3:1.1.					
31601012	5	55	theme	monosaccharide	675:688	arg1	follows					712:718	follows	712:718	follows	712:718	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	5	55	theme	monosaccharide	675:688	arg1	residue					690:696	AX-I-3b monosaccharide residue	667:696	AX-I-3b monosaccharide residue	667:696	Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: →2,3,4)-β-d-Xylp-(1→, →4)-β-d-Arap-(1→, →4)-β-d-Glcp-(1→.					
31601012	9	56	theme	lung	1370:1373	arg1	mice					1382:1385	lung cancer mice	1370:1385	lung cancer mice	1370:1385	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	9	57	theme	immunomodulatory	1419:1434	arg1	activity					1436:1443	immunomodulatory activity	1419:1443	immunomodulatory activity	1419:1443	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	0	58	theme	Hemicellulose	72:84	arg1	Polysaccharide					86:99	Hemicellulose Polysaccharide	72:99	Hemicellulose Polysaccharide	72:99	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	59	from	Astragalus	106:115	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	59	from	Astragalus	106:115	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	59	from	Astragalus	106:115	arg1	Antitumor					30:38	Antitumor	30:38	Antitumor	30:38	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	0	59	from	Astragalus	106:115	arg1	Activities					58:67	Immunological Activities	44:67	Immunological Activities	44:67	Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.					
31601012	1	60	used	used	183:186	arg2	radix					155:159	radix	155:159	radix	155:159	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	60	used	used	183:186	arg2	radix					148:152	Astragalus radix	137:152	Astragalus radix (radix)	137:160	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	3	61	theme	hemicellulose	392:404	arg1	AX-I-3b					421:427	hemicellulose polysaccharide AX-I-3b	392:427	hemicellulose polysaccharide AX-I-3b	392:427	We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction.					
31601012	1	62	theme	herb	231:234	arg1	radix					248:252	radix	248:252	radix	248:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	62	theme	herb	231:234	arg1	waste					271:275	a waste	269:275	a waste of resources	269:288	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	1	62	theme	herb	231:234	arg1	residues					236:243	the herb residues	227:243	the herb residues of radix	227:252	Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources.					
31601012	9	63	theme	serum	1345:1349	arg1	levels					1360:1365	serum cytokine levels	1345:1365	serum cytokine levels	1345:1365	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
31601012	10	64	theme	immunomodulatory	1512:1527	arg1	AX-I-3b					1484:1490	AX-I-3b	1484:1490	AX-I-3b	1484:1490	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	10	64	theme	immunomodulatory	1512:1527	arg1	agent					1529:1533	an antitumor and immunomodulatory agent	1495:1533	an antitumor and immunomodulatory agent	1495:1533	In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.					
31601012	9	65	theme	lymphocyte	1322:1331	arg1	subsets					1333:1339	spleen lymphocyte subsets	1315:1339	spleen lymphocyte subsets	1315:1339	Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity.					
30578646	3	0	theme	tumor	670:674	arg1	growth					676:681	in vivo tumor growth	662:681	in vivo tumor growth	662:681	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	1	theme	MC38-MOCK	707:715	arg1	cells					717:721	the control MC38-MOCK cells	695:721	the control MC38-MOCK cells	695:721	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	2	theme	cell	532:535	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	7	3	theme	therapeutics	1412:1423	arg1	design					1389:1394	the design	1385:1394	the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile	1385:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	2	4	theme	colorectal	264:273	arg1	cancer					275:280	colorectal cancer	264:280	colorectal cancer	264:280	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	5	5	from	able	981:984	arg1	addition					947:954	addition	947:954	addition	947:954	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	7	6	theme	tumor	1447:1451	arg1	profile					1467:1473	the tumor glycosylation profile	1443:1473	the tumor glycosylation profile	1443:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	4	7	theme	tumor	837:841	arg1	microenvironment					843:858	the tumor microenvironment	833:858	the tumor microenvironment only	833:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	6	8	theme	cancer	1158:1163	arg1	cohort					1165:1170	a human colorectal cancer cohort	1139:1170	a human colorectal cancer cohort	1139:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	2	9	theme	complete	306:313	arg1	desialylation					321:333	complete tumor desialylation	306:333	complete tumor desialylation	306:333	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	4	10	theme	lymph	911:915	arg1	nodes					917:921	tumor-draining lymph nodes	896:921	tumor-draining lymph nodes	896:921	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	6	11	theme	human	1141:1145	arg1	cohort					1165:1170	a human colorectal cancer cohort	1139:1170	a human colorectal cancer cohort	1139:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	1	12	theme	immune	203:208	arg1	evasion					210:216	tumor immune evasion	197:216	tumor immune evasion	197:216	Sialylated glycan structures are known for their immunomodulatory capacities and their contribution to tumor immune evasion.					
30578646	3	13	theme	sialic	590:595	arg1	MC38-Sianull					604:615	MC38-Sianull	604:615	MC38-Sianull	604:615	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	13	theme	sialic	590:595	arg1	acids					597:601	sialic acids	590:601	sialic acids (MC38-Sianull )	590:617	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	4	14	theme	cell	876:879	arg1	frequencies					881:891	immune cell frequencies	869:891	immune cell frequencies in tumor-draining lymph nodes	869:921	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	4	15	from	frequencies	881:891	arg1	nodes					917:921	tumor-draining lymph nodes	896:921	tumor-draining lymph nodes	896:921	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	2	16	theme	sialylation	249:259	arg1	role					232:235	the role	228:235	the role of aberrant sialylation in colorectal cancer	228:280	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	2	16	theme	sialylation	249:259	arg1	consequences					290:301	the consequences	286:301	the consequences of complete tumor desialylation on anti-tumor immunity	286:356	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	7	17	theme	cancer	1342:1347	arg1	growth					1355:1360	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	0	18	theme	CD8+	71:74	arg1	apoptosis					83:91	CD8+ T cell apoptosis	71:91	CD8+ T cell apoptosis	71:91	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	0	19	theme	cell	78:81	arg1	apoptosis					83:91	CD8+ T cell apoptosis	71:91	CD8+ T cell apoptosis	71:91	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	5	20	theme	MC38-Sianull	957:968	arg1	cells					970:974	MC38-Sianull cells	957:974	MC38-Sianull cells	957:974	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	4	21	theme	cells	767:771	arg1	growth					744:749	This enhanced tumor growth	724:749	This enhanced tumor growth of MC38-Sianull cells	724:771	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	2	22	from	role	232:235	arg1	immunity					349:356	anti-tumor immunity	338:356	anti-tumor immunity	338:356	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	2	22	from	role	232:235	arg1	cancer					275:280	colorectal cancer	264:280	colorectal cancer	264:280	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	6	23	theme	low	1062:1064	arg1	expression					1076:1085	low CMAS gene expression	1062:1085	low CMAS gene expression	1062:1085	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	7	24	theme	desialylation	1314:1326	arg1	effect					1289:1294	a detrimental effect	1275:1294	a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile	1275:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	2	25	theme	aberrant	240:247	arg1	sialylation					249:259	aberrant sialylation	240:259	aberrant sialylation	240:259	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	4	26	theme	tumor	738:742	arg1	growth					744:749	This enhanced tumor growth	724:749	This enhanced tumor growth of MC38-Sianull cells	724:771	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	3	27	theme	key	457:459	arg1	enzyme					461:466	a key enzyme	455:466	a key enzyme in the sialylation pathway	455:493	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	0	28	theme	sialic	14:19	arg1	metabolism					26:35	sialic acid metabolism	14:35	sialic acid metabolism	14:35	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	1	29	theme	Sialylated	94:103	arg1	structures					112:121	Sialylated glycan structures	94:121	Sialylated glycan structures	94:121	Sialylated glycan structures are known for their immunomodulatory capacities and their contribution to tumor immune evasion.					
30578646	7	30	theme	complete	1299:1306	arg1	desialylation					1314:1326	complete tumor desialylation	1299:1326	complete tumor desialylation	1299:1326	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	4	31	theme	CD8+	806:809	arg1	cell					813:816	CD8+ T cell	806:816	decreased CD8+ T cell frequencies in the tumor microenvironment only	796:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	0	32	theme	metabolism	26:35	arg1	Disruption					0:9	Disruption	0:9	Disruption of sialic acid metabolism	0:35	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	5	33	theme	cell	1003:1006	arg1	apoptosis					1008:1016	CD8+ T cell apoptosis	996:1016	CD8+ T cell apoptosis	996:1016	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	3	34	theme	CRISPR/Cas9-mediated	397:416	arg1	knock					418:422	CRISPR/Cas9-mediated knock	397:422	CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line	397:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	4	35	theme	cell	813:816	arg1	frequencies					818:828	decreased CD8+ T cell frequencies	796:828	decreased CD8+ T cell frequencies in the tumor microenvironment only	796:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	3	36	theme	sialylation	475:485	arg1	pathway					487:493	the sialylation pathway	471:493	the sialylation pathway	471:493	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	0	37	theme	tumor	44:48	arg1	growth					50:55	tumor growth	44:55	tumor growth	44:55	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	7	38	theme	tumor	1349:1353	arg1	growth					1355:1360	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	6	39	from	survival	1127:1134	arg1	cohort					1165:1170	a human colorectal cancer cohort	1139:1170	a human colorectal cancer cohort	1139:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	5	40	from	addition	947:954	arg1	able					981:984	able	981:984	able	981:984	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	3	41	theme	cancer	520:525	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	6	42	theme	reduced	1103:1109	arg1	survival					1127:1134	reduced recurrence-free survival	1103:1134	reduced recurrence-free survival in a human colorectal cancer cohort	1103:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	5	43	theme	CD8+	996:999	arg1	apoptosis					1008:1016	CD8+ T cell apoptosis	996:1016	CD8+ T cell apoptosis	996:1016	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	6	44	theme	gene	1071:1074	arg1	expression					1076:1085	low CMAS gene expression	1062:1085	low CMAS gene expression	1062:1085	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	3	45	theme	MC38	527:530	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	46	dep	in	662:663	arg1	vivo					665:668	vivo	665:668	vivo	665:668	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	7	47	theme	novel	1399:1403	arg1	therapeutics					1412:1423	novel cancer therapeutics	1399:1423	novel cancer therapeutics aimed at altering the tumor glycosylation profile	1399:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	7	48	theme	glycosylation	1453:1465	arg1	profile					1467:1473	the tumor glycosylation profile	1443:1473	the tumor glycosylation profile	1443:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	3	49	dep	abrogated	553:561	arg1	increased					652:660	increased	652:660	increased in vivo tumor growth compared to the control MC38-MOCK cells	652:721	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	50	theme	colorectal	509:518	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	51	theme	control	699:705	arg1	cells					717:721	the control MC38-MOCK cells	695:721	the control MC38-MOCK cells	695:721	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	2	52	theme	tumor	315:319	arg1	desialylation					321:333	complete tumor desialylation	306:333	complete tumor desialylation	306:333	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	7	53	theme	cancer	1405:1410	arg1	therapeutics					1412:1423	novel cancer therapeutics	1399:1423	novel cancer therapeutics aimed at altering the tumor glycosylation profile	1399:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	1	54	theme	tumor	197:201	arg1	evasion					210:216	tumor immune evasion	197:216	tumor immune evasion	197:216	Sialylated glycan structures are known for their immunomodulatory capacities and their contribution to tumor immune evasion.					
30578646	3	55	theme	cell	563:566	arg1	expression					576:585	cell surface expression	563:585	cell surface expression of sialic acids (MC38-Sianull )	563:617	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	4	56	theme	tumor-draining	896:909	arg1	nodes					917:921	tumor-draining lymph nodes	896:921	tumor-draining lymph nodes	896:921	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	3	57	theme	surface	568:574	arg1	expression					576:585	cell surface expression	563:585	cell surface expression of sialic acids (MC38-Sianull )	563:617	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	1	58	theme	glycan	105:110	arg1	structures					112:121	Sialylated glycan structures	94:121	Sialylated glycan structures	94:121	Sialylated glycan structures are known for their immunomodulatory capacities and their contribution to tumor immune evasion.					
30578646	4	59	theme	immune	869:874	arg1	frequencies					881:891	immune cell frequencies	869:891	immune cell frequencies in tumor-draining lymph nodes	869:921	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	6	60	theme	work	1214:1217	arg1	relevance					1197:1205	the clinical relevance	1184:1205	the clinical relevance of our work	1184:1217	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	5	61	theme	antigen-independent	1024:1042	arg1	manner					1044:1049	an antigen-independent manner	1021:1049	an antigen-independent manner	1021:1049	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	3	62	from	enzyme	461:466	arg1	pathway					487:493	the sialylation pathway	471:493	the sialylation pathway	471:493	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	3	63	theme	acids	597:601	arg1	expression					576:585	cell surface expression	563:585	cell surface expression of sialic acids (MC38-Sianull )	563:617	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	6	64	theme	clinical	1188:1195	arg1	relevance					1197:1205	the clinical relevance	1184:1205	the clinical relevance of our work	1184:1217	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	0	65	theme	T	76:76	arg1	apoptosis					83:91	CD8+ T cell apoptosis	71:91	CD8+ T cell apoptosis	71:91	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	3	66	from	knock	418:422	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	1	67	theme	immunomodulatory	143:158	arg1	capacities					160:169	their immunomodulatory capacities	137:169	their immunomodulatory capacities	137:169	Sialylated glycan structures are known for their immunomodulatory capacities and their contribution to tumor immune evasion.					
30578646	2	68	from	consequences	290:301	arg1	immunity					349:356	anti-tumor immunity	338:356	anti-tumor immunity	338:356	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	2	68	from	consequences	290:301	arg1	cancer					275:280	colorectal cancer	264:280	colorectal cancer	264:280	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	7	69	theme	first	1264:1268	arg1	time					1270:1273	the first time	1260:1273	the first time	1260:1273	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	6	70	theme	CMAS	1066:1069	arg1	expression					1076:1085	low CMAS gene expression	1062:1085	low CMAS gene expression	1062:1085	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	4	71	theme	MC38-Sianull	754:765	arg1	cells					767:771	MC38-Sianull cells	754:771	MC38-Sianull cells	754:771	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	4	72	from	frequencies	818:828	arg1	microenvironment					843:858	the tumor microenvironment	833:858	the tumor microenvironment only	833:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	7	73	gly	desialylation	1314:1326	arg1	cancer					1342:1347	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	7	73	gly	desialylation	1314:1326	arg1	tumor					1349:1353	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	7	74	theme	tumor	1308:1312	arg1	desialylation					1314:1326	complete tumor desialylation	1299:1326	complete tumor desialylation	1299:1326	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	3	75	theme	CMAS	435:438	arg1	gene					440:443	the CMAS gene	431:443	the CMAS gene	431:443	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	0	76	theme	acid	21:24	arg1	metabolism					26:35	sialic acid metabolism	14:35	sialic acid metabolism	14:35	Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis.					
30578646	4	77	theme	enhanced	729:736	arg1	growth					744:749	This enhanced tumor growth	724:749	This enhanced tumor growth of MC38-Sianull cells	724:771	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	6	78	theme	colorectal	1147:1156	arg1	cohort					1165:1170	a human colorectal cancer cohort	1139:1170	a human colorectal cancer cohort	1139:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	5	79	theme	T	1001:1001	arg1	apoptosis					1008:1016	CD8+ T cell apoptosis	996:1016	CD8+ T cell apoptosis	996:1016	In addition, MC38-Sianull cells were able to induce CD8+ T cell apoptosis in an antigen-independent manner.					
30578646	7	80	theme	detrimental	1277:1287	arg1	effect					1289:1294	a detrimental effect	1275:1294	a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile	1275:1473	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	4	81	theme	decreased	796:804	arg1	frequencies					818:828	decreased CD8+ T cell frequencies	796:828	decreased CD8+ T cell frequencies in the tumor microenvironment only	796:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	7	82	from	effect	1289:1294	arg1	growth					1355:1360	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	3	83	theme	in	662:663	arg1	growth					676:681	in vivo tumor growth	662:681	in vivo tumor growth	662:681	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30578646	4	84	theme	T	811:811	arg1	cell					813:816	CD8+ T cell	806:816	decreased CD8+ T cell frequencies in the tumor microenvironment only	796:863	This enhanced tumor growth of MC38-Sianull cells could be attributed to decreased CD8+ T cell frequencies in the tumor microenvironment only, as immune cell frequencies in tumor-draining lymph nodes remained unaffected.					
30578646	2	85	theme	anti-tumor	338:347	arg1	immunity					349:356	anti-tumor immunity	338:356	anti-tumor immunity	338:356	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	6	86	theme	recurrence-free	1111:1125	arg1	survival					1127:1134	reduced recurrence-free survival	1103:1134	reduced recurrence-free survival in a human colorectal cancer cohort	1103:1170	Moreover, low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort, supporting the clinical relevance of our work.					
30578646	2	87	theme	desialylation	321:333	arg1	role					232:235	the role	228:235	the role of aberrant sialylation in colorectal cancer	228:280	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	2	87	theme	desialylation	321:333	arg1	consequences					290:301	the consequences	286:301	the consequences of complete tumor desialylation on anti-tumor immunity	286:356	However, the role of aberrant sialylation in colorectal cancer and the consequences of complete tumor desialylation on anti-tumor immunity remain unstudied.					
30578646	7	88	theme	colorectal	1331:1340	arg1	growth					1355:1360	colorectal cancer tumor growth	1331:1360	colorectal cancer tumor growth	1331:1360	Together, these results demonstrate for the first time a detrimental effect of complete tumor desialylation on colorectal cancer tumor growth, which greatly impacts the design of novel cancer therapeutics aimed at altering the tumor glycosylation profile.					
30578646	3	89	theme	mouse	503:507	arg1	line					537:540	the mouse colorectal cancer MC38 cell line	499:540	the mouse colorectal cancer MC38 cell line	499:540	Here, we report that CRISPR/Cas9-mediated knock out of the CMAS gene, encoding a key enzyme in the sialylation pathway, in the mouse colorectal cancer MC38 cell line completely abrogated cell surface expression of sialic acids (MC38-Sianull ) and, unexpectedly, significantly increased in vivo tumor growth compared to the control MC38-MOCK cells.					
30291933	8	0	used	used	1324:1327	arg2	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	0	used	used	1324:1327	arg2	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	0	1	theme	polysaccharides	84:98	arg1	antioxidant					41:51	antioxidant	41:51	antioxidant	41:51	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	1	theme	polysaccharides	84:98	arg1	activities					70:79	hypoglycemic activities	57:79	hypoglycemic activities	57:79	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	1	theme	polysaccharides	84:98	arg1	characterization					23:38	characterization	23:38	characterization	23:38	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	1	theme	polysaccharides	84:98	arg1	modification					9:20	modification	9:20	modification	9:20	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	2	from	modification	9:20	arg1	pallidum					115:122	Sargassum pallidum	105:122	Sargassum pallidum	105:122	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	6	3	theme	ABTS	872:875	arg1	scavenging					885:894	ABTS radical scavenging	872:894	ABTS radical scavenging	872:894	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	1	4	theme	Sargassum	261:269	arg1	SPP					297:299	SPP	297:299	SPP	297:299	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	1	4	theme	Sargassum	261:269	arg1	polysaccharides					280:294	Sargassum pallidum polysaccharides	261:294	Sargassum pallidum polysaccharides (SPP)	261:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	4	5	theme	substitution	569:580	arg1	degrees					558:564	The degrees	554:564	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8	554:620	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	1	6	theme	pallidum	271:278	arg1	SPP					297:299	SPP	297:299	SPP	297:299	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	1	6	theme	pallidum	271:278	arg1	polysaccharides					280:294	Sargassum pallidum polysaccharides	261:294	Sargassum pallidum polysaccharides (SPP)	261:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	8	7	theme	hypoglycemic	1253:1264	arg1	activities					1266:1275	the antioxidant and hypoglycemic activities	1233:1275	the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent	1233:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	6	8	theme	sulfated	809:816	arg1	polysaccharides					818:832	these sulfated polysaccharides	803:832	these sulfated polysaccharides	803:832	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	8	9	theme	sulfated	1197:1204	arg1	modification					1206:1217	appropriately sulfated modification	1183:1217	appropriately sulfated modification	1183:1217	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	1	10	theme	polysaccharides	280:294	arg1	activities					247:256	the in vitro antioxidant and hypoglycemic activities	205:256	the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	205:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	5	11	theme	SPP	792:794	arg1	component					730:738	chemical component	721:738	chemical component	721:738	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	5	11	theme	SPP	792:794	arg1	weight					751:756	molecular weight	741:756	molecular weight	741:756	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	5	11	theme	SPP	792:794	arg1	composition					777:787	monosaccharide composition	762:787	monosaccharide composition of SPP	762:794	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	6	12	theme	inhibitory	956:965	arg1	activity					967:974	the best α-glucosidase inhibitory activity	933:974	the best α-glucosidase inhibitory activity	933:974	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	8	13	theme	S.	1280:1281	arg1	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	13	theme	S.	1280:1281	arg1	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	6	14	theme	best	858:861	arg1	DPPH					863:866	DPPH	863:866	DPPH	863:866	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	2	15	theme	chlorosulfonic	384:397	arg1	method					413:418	chlorosulfonic acid-pyridine method	384:418	chlorosulfonic acid-pyridine method	384:418	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	8	16	theme	alternative	1335:1345	arg1	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	16	theme	alternative	1335:1345	arg1	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	0	17	from	antioxidant	41:51	arg1	pallidum					115:122	Sargassum pallidum	105:122	Sargassum pallidum	105:122	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	2	18	theme	acid-pyridine	399:411	arg1	method					413:418	chlorosulfonic acid-pyridine method	384:418	chlorosulfonic acid-pyridine method	384:418	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	8	19	theme	polysaccharides	1292:1306	arg1	activities					1266:1275	the antioxidant and hypoglycemic activities	1233:1275	the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent	1233:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	20	theme	antioxidant	1237:1247	arg1	activities					1266:1275	the antioxidant and hypoglycemic activities	1233:1275	the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent	1233:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	5	21	theme	Sulfated	671:678	arg1	modification					680:691	Sulfated modification	671:691	Sulfated modification	671:691	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	6	22	theme	-HepG2	1045:1050	arg1	cells					1052:1056	insulin resistant (IR)-HepG2 cells	1023:1056	insulin resistant (IR)-HepG2 cells	1023:1056	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	1	23	theme	sulfated	180:187	arg1	modification					189:200	sulfated modification	180:200	sulfated modification	180:200	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	8	24	theme	pallidum	1283:1290	arg1	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	24	theme	pallidum	1283:1290	arg1	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	4	25	theme	S-SPP1-6	600:607	arg1	DS					583:584	DS	583:584	DS	583:584	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	4	25	theme	S-SPP1-6	600:607	arg1	substitution					569:580	substitution	569:580	substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8	569:620	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	6	26	dep	DPPH	863:866	arg1	activities					896:905	activities	896:905	activities	896:905	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	6	26	dep	DPPH	863:866	arg1	the					854:856	the	854:856	the	854:856	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	1	27	theme	modification	189:200	arg1	effect					170:175	the effect	166:175	the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	166:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	5	28	theme	molecular	741:749	arg1	weight					751:756	molecular weight	741:756	molecular weight	741:756	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	2	29	theme	sulfated	309:316	arg1	S-SPP1-6					341:348	S-SPP1-6	341:348	S-SPP1-6	341:348	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	29	theme	sulfated	309:316	arg1	S-SPP1-4					331:338	S-SPP1-4	331:338	S-SPP1-4	331:338	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	29	theme	sulfated	309:316	arg1	derivatives					318:328	Three sulfated derivatives	303:328	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8)	303:362	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	29	theme	sulfated	309:316	arg1	S-SPP1-8					354:361	S-SPP1-8	354:361	S-SPP1-8	354:361	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	8	30	theme	antioxidant	1369:1379	arg1	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	30	theme	antioxidant	1369:1379	arg1	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	4	31	theme	S-SPP1-8	613:620	arg1	DS					583:584	DS	583:584	DS	583:584	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	4	31	theme	S-SPP1-8	613:620	arg1	substitution					569:580	substitution	569:580	substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8	569:620	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	2	32	dep	derivatives	318:328	arg1	S-SPP1-6					341:348	S-SPP1-6	341:348	S-SPP1-6	341:348	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	32	dep	derivatives	318:328	arg1	S-SPP1-4					331:338	S-SPP1-4	331:338	S-SPP1-4	331:338	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	32	dep	derivatives	318:328	arg1	derivatives					318:328	Three sulfated derivatives	303:328	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8)	303:362	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	2	32	dep	derivatives	318:328	arg1	S-SPP1-8					354:361	S-SPP1-8	354:361	S-SPP1-8	354:361	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	6	33	from	activity	967:974	arg1	cells					1052:1056	insulin resistant (IR)-HepG2 cells	1023:1056	insulin resistant (IR)-HepG2 cells	1023:1056	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	6	33	from	activity	967:974	arg1	consumption					1008:1018	glucose consumption	1000:1018	glucose consumption	1000:1018	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	2	34	theme	reaction	436:443	arg1	conditions					445:454	different reaction conditions	426:454	different reaction conditions	426:454	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	1	35	theme	in	209:210	arg1	activities					247:256	the in vitro antioxidant and hypoglycemic activities	205:256	the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	205:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	8	36	theme	natural	1361:1367	arg1	antioxidant					1369:1379	natural antioxidant	1361:1379	natural antioxidant	1361:1379	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	3	37	theme	Physicochemical	457:471	arg1	characterization					473:488	Physicochemical characterization	457:488	Physicochemical characterization	457:488	Physicochemical characterization indicated that sulfated modification had successfully occurred.					
30291933	2	38	theme	different	426:434	arg1	conditions					445:454	different reaction conditions	426:454	different reaction conditions	426:454	Three sulfated derivatives (S-SPP1-4, S-SPP1-6 and S-SPP1-8) were prepared using chlorosulfonic acid-pyridine method under different reaction conditions.					
30291933	6	39	theme	resistant	1031:1039	arg1	cells					1052:1056	insulin resistant (IR)-HepG2 cells	1023:1056	insulin resistant (IR)-HepG2 cells	1023:1056	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	5	40	theme	monosaccharide	762:775	arg1	composition					777:787	monosaccharide composition	762:787	monosaccharide composition of SPP	762:794	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	6	41	dep	resistant	1031:1039	arg1	IR					1042:1043	IR	1042:1043	IR	1042:1043	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	7	42	theme	sulfated	1080:1087	arg1	derivatives					1089:1099	all the sulfated derivatives	1072:1099	all the sulfated derivatives	1072:1099	Furthermore, all the sulfated derivatives exhibited better hypoglycemic ability than native SPP.					
30291933	3	43	theme	sulfated	505:512	arg1	modification					514:525	sulfated modification	505:525	sulfated modification	505:525	Physicochemical characterization indicated that sulfated modification had successfully occurred.					
30291933	0	44	theme	hypoglycemic	57:68	arg1	activities					70:79	hypoglycemic activities	57:79	hypoglycemic activities	57:79	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	5	45	from	changes	710:716	arg1	component					730:738	chemical component	721:738	chemical component	721:738	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	5	45	from	changes	710:716	arg1	weight					751:756	molecular weight	741:756	molecular weight	741:756	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	5	45	from	changes	710:716	arg1	composition					777:787	monosaccharide composition	762:787	monosaccharide composition of SPP	762:794	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	7	46	theme	hypoglycemic	1118:1129	arg1	ability					1131:1137	better hypoglycemic ability	1111:1137	better hypoglycemic ability	1111:1137	Furthermore, all the sulfated derivatives exhibited better hypoglycemic ability than native SPP.					
30291933	0	47	from	activities	70:79	arg1	pallidum					115:122	Sargassum pallidum	105:122	Sargassum pallidum	105:122	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	6	48	theme	α-glucosidase	942:954	arg1	activity					967:974	the best α-glucosidase inhibitory activity	933:974	the best α-glucosidase inhibitory activity	933:974	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	7	49	theme	native	1144:1149	arg1	SPP					1151:1153	native SPP	1144:1153	native SPP	1144:1153	Furthermore, all the sulfated derivatives exhibited better hypoglycemic ability than native SPP.					
30291933	6	50	from	promoting-effect	980:995	arg1	cells					1052:1056	insulin resistant (IR)-HepG2 cells	1023:1056	insulin resistant (IR)-HepG2 cells	1023:1056	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	6	50	from	promoting-effect	980:995	arg1	consumption					1008:1018	glucose consumption	1000:1018	glucose consumption	1000:1018	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	4	51	theme	S-SPP1-4	590:597	arg1	DS					583:584	DS	583:584	DS	583:584	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	4	51	theme	S-SPP1-4	590:597	arg1	substitution					569:580	substitution	569:580	substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8	569:620	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	5	52	theme	chemical	721:728	arg1	component					730:738	chemical component	721:738	chemical component	721:738	Sulfated modification resulted in some changes in chemical component, molecular weight and monosaccharide composition of SPP.					
30291933	4	53	dep	1.19	665:668	arg1	to					662:663	to	662:663	to	662:663	The degrees of substitution (DS) of S-SPP1-4, S-SPP1-6 and S-SPP1-8 were determined to be ranging from 0.85 to 1.19.					
30291933	8	54	theme	agent	1398:1402	arg1	derivative					1347:1356	an alternative derivative	1332:1356	an alternative derivative of natural antioxidant and hypoglycemic agent	1332:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	54	theme	agent	1398:1402	arg1	polysaccharides					1292:1306	S. pallidum polysaccharides	1280:1306	S. pallidum polysaccharides	1280:1306	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	8	55	theme	hypoglycemic	1385:1396	arg1	agent					1398:1402	hypoglycemic agent	1385:1402	hypoglycemic agent	1385:1402	These results suggest that appropriately sulfated modification could enhance the antioxidant and hypoglycemic activities of S. pallidum polysaccharides, which might be used as an alternative derivative of natural antioxidant and hypoglycemic agent.					
30291933	1	56	dep	in	209:210	arg1	vitro					212:216	vitro	212:216	vitro	212:216	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	1	57	theme	antioxidant	218:228	arg1	activities					247:256	the in vitro antioxidant and hypoglycemic activities	205:256	the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	205:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	7	58	theme	better	1111:1116	arg1	ability					1131:1137	better hypoglycemic ability	1111:1137	better hypoglycemic ability	1111:1137	Furthermore, all the sulfated derivatives exhibited better hypoglycemic ability than native SPP.					
30291933	0	59	from	pallidum	115:122	arg1	polysaccharides					84:98	polysaccharides	84:98	polysaccharides from Sargassum pallidum	84:122	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	59	from	pallidum	115:122	arg1	antioxidant					41:51	antioxidant	41:51	antioxidant	41:51	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	59	from	pallidum	115:122	arg1	activities					70:79	hypoglycemic activities	57:79	hypoglycemic activities	57:79	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	59	from	pallidum	115:122	arg1	characterization					23:38	characterization	23:38	characterization	23:38	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	0	59	from	pallidum	115:122	arg1	modification					9:20	modification	9:20	modification	9:20	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	1	60	theme	study	141:145	arg1	aim					129:131	The aim	125:131	The aim of this study	125:145	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	6	61	theme	glucose	1000:1006	arg1	consumption					1008:1018	glucose consumption	1000:1018	glucose consumption	1000:1018	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	6	62	theme	radical	877:883	arg1	scavenging					885:894	ABTS radical scavenging	872:894	ABTS radical scavenging	872:894	Among these sulfated polysaccharides, S-SPP1-4 exhibited the best DPPH and ABTS radical scavenging activities, while S-SPP1-8 exhibited the best α-glucosidase inhibitory activity and promoting-effect on glucose consumption in insulin resistant (IR)-HepG2 cells.					
30291933	1	63	theme	hypoglycemic	234:245	arg1	activities					247:256	the in vitro antioxidant and hypoglycemic activities	205:256	the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	205:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30291933	0	64	from	characterization	23:38	arg1	pallidum					115:122	Sargassum pallidum	105:122	Sargassum pallidum	105:122	Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum.					
30291933	1	65	from	effect	170:175	arg1	activities					247:256	the in vitro antioxidant and hypoglycemic activities	205:256	the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP)	205:300	The aim of this study was to investigate the effect of sulfated modification on the in vitro antioxidant and hypoglycemic activities of Sargassum pallidum polysaccharides (SPP).					
30910671	3	0	theme	uronic	599:604	arg1	contents					611:618	uronic acid contents	599:618	uronic acid contents	599:618	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	0	theme	uronic	599:604	arg1	%					629:629	20.20%	624:629	20.20%	624:629	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	0	theme	uronic	599:604	arg1	β-glucan					576:583	the β-glucan	572:583	the β-glucan	572:583	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	0	theme	uronic	599:604	arg1	protein					586:592	protein	586:592	protein	586:592	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	4	1	theme	resistance	835:844	arg1	activity					846:853	H2O2-injury resistance activity	823:853	H2O2-injury resistance activity	823:853	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	5	2	theme	different	1002:1010	arg1	types					1012:1016	different types	1002:1016	different types	1002:1016	Monosaccharide composition analysis shows that P. nameko belongs to heteropolysaccharides, which consists of galactose, glucose, and mannose with different types and ratios, and the molecular weight are distributed at 4.40-333.49kDa.					
30910671	0	3	from	analysis	9:16	arg1	Pholiota					91:98	Pholiota	91:98	Pholiota	91:98	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	4	4	contain	has	788:790	arg2	ability					811:817	better antioxidant ability	792:817	better antioxidant ability	792:817	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	4	4	contain	has	788:790	arg1	PNP-80					781:786	PNP-80	781:786	PNP-80	781:786	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	4	4	contain	has	788:790	arg2	activity					846:853	H2O2-injury resistance activity	823:853	H2O2-injury resistance activity	823:853	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	5	5	theme	composition	871:881	arg1	analysis					883:890	Monosaccharide composition analysis	856:890	Monosaccharide composition analysis	856:890	Monosaccharide composition analysis shows that P. nameko belongs to heteropolysaccharides, which consists of galactose, glucose, and mannose with different types and ratios, and the molecular weight are distributed at 4.40-333.49kDa.					
30910671	1	6	dep	ethanol	277:283	arg1	v/v					305:307	v/v	305:307	v/v	305:307	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	6	dep	ethanol	277:283	arg1	%					302:302	80%	300:302	80% (v/v)	300:308	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	6	dep	ethanol	277:283	arg1	%					288:288	40%	286:288	40%	286:288	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	6	dep	ethanol	277:283	arg1	%					293:293	60%	291:293	60%	291:293	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	7	theme	gradient	250:257	arg1	concentrations					259:272	gradient concentrations	250:272	gradient concentrations of ethanol (40%, 60%, and 80% (v/v))	250:309	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	0	8	from	activities	56:65	arg1	Pholiota					91:98	Pholiota	91:98	Pholiota	91:98	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	3	9	theme	PNP-60	501:506	arg1	%					529:529	45.12%	524:529	45.12%	524:529	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	9	theme	PNP-60	501:506	arg1	contents					481:488	the polysaccharide contents	462:488	the polysaccharide contents of PNP-40, PNP-60, and PNP-80	462:518	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	0	10	theme	fractional	110:119	arg1	precipitation					121:133	fractional precipitation	110:133	fractional precipitation	110:133	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	3	11	theme	acid	606:609	arg1	contents					611:618	uronic acid contents	599:618	uronic acid contents	599:618	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	11	theme	acid	606:609	arg1	%					629:629	20.20%	624:629	20.20%	624:629	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	11	theme	acid	606:609	arg1	β-glucan					576:583	the β-glucan	572:583	the β-glucan	572:583	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	11	theme	acid	606:609	arg1	protein					586:592	protein	586:592	protein	586:592	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	12	theme	PNP-40	493:498	arg1	%					529:529	45.12%	524:529	45.12%	524:529	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	12	theme	PNP-40	493:498	arg1	contents					481:488	the polysaccharide contents	462:488	the polysaccharide contents of PNP-40, PNP-60, and PNP-80	462:518	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	2	13	theme	physicochemical	316:330	arg1	properties					332:341	The physicochemical properties	312:341	The physicochemical properties	312:341	The physicochemical properties, functional group composition, moisture-preserving, and antioxidant ability were determined.					
30910671	4	14	theme	moisture	754:761	arg1	absorption					763:772	better moisture absorption	747:772	better moisture absorption	747:772	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	3	15	theme	PNP-80	513:518	arg1	%					529:529	45.12%	524:529	45.12%	524:529	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	15	theme	PNP-80	513:518	arg1	contents					481:488	the polysaccharide contents	462:488	the polysaccharide contents of PNP-40, PNP-60, and PNP-80	462:518	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	1	16	theme	polysaccharide	184:197	arg1	PNP-80					230:235	PNP-80	230:235	PNP-80	230:235	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	16	theme	polysaccharide	184:197	arg1	PNP-40					210:215	PNP-40	210:215	PNP-40	210:215	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	16	theme	polysaccharide	184:197	arg1	fractions					199:207	Pholiota nameko (P. nameko) polysaccharide fractions	156:207	Pholiota nameko (P. nameko) polysaccharide fractions	156:207	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	16	theme	polysaccharide	184:197	arg1	PNP-60					218:223	PNP-60	218:223	PNP-60	218:223	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	0	17	theme	Chemical	0:7	arg1	analysis					9:16	Chemical analysis	0:16	Chemical analysis	0:16	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	4	18	theme	antioxidant	799:809	arg1	ability					811:817	better antioxidant ability	792:817	better antioxidant ability	792:817	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	4	19	theme	H2O2-injury	823:833	arg1	activity					846:853	H2O2-injury resistance activity	823:853	H2O2-injury resistance activity	823:853	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	1	20	theme	ethanol	277:283	arg1	concentrations					259:272	gradient concentrations	250:272	gradient concentrations of ethanol (40%, 60%, and 80% (v/v))	250:309	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	0	21	from	Pholiota	91:98	arg1	activities					56:65	antioxidant activities	44:65	antioxidant activities	44:65	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	0	21	from	Pholiota	91:98	arg1	analysis					9:16	Chemical analysis	0:16	Chemical analysis	0:16	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	0	21	from	Pholiota	91:98	arg1	moisture-preserving					19:37	moisture-preserving	19:37	moisture-preserving	19:37	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	0	21	from	Pholiota	91:98	arg1	polysaccharides					70:84	polysaccharides	70:84	polysaccharides from Pholiota	70:98	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	2	22	theme	group	355:359	arg1	composition					361:371	functional group composition	344:371	functional group composition	344:371	The physicochemical properties, functional group composition, moisture-preserving, and antioxidant ability were determined.					
30910671	2	23	theme	functional	344:353	arg1	composition					361:371	functional group composition	344:371	functional group composition	344:371	The physicochemical properties, functional group composition, moisture-preserving, and antioxidant ability were determined.					
30910671	6	24	theme	active	1212:1217	arg1	ingredients					1219:1229	their active ingredients	1206:1229	their active ingredients	1206:1229	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	4	25	theme	better	792:797	arg1	ability					811:817	better antioxidant ability	792:817	better antioxidant ability	792:817	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	6	26	theme	polysaccharide	1118:1131	arg1	fractions					1133:1141	different polysaccharide fractions	1108:1141	different polysaccharide fractions	1108:1141	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	4	27	contain	has	743:745	arg1	PNP-60					736:741	PNP-60	736:741	PNP-60	736:741	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	4	27	contain	has	743:745	arg2	absorption					763:772	better moisture absorption	747:772	better moisture absorption	747:772	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	0	28	theme	antioxidant	44:54	arg1	activities					56:65	antioxidant activities	44:65	antioxidant activities	44:65	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	6	29	theme	different	1108:1116	arg1	fractions					1133:1141	different polysaccharide fractions	1108:1141	different polysaccharide fractions	1108:1141	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	2	30	theme	antioxidant	399:409	arg1	ability					411:417	antioxidant ability	399:417	antioxidant ability	399:417	The physicochemical properties, functional group composition, moisture-preserving, and antioxidant ability were determined.					
30910671	6	31	contain	have	1143:1146	arg2	potential					1152:1160	the potential to be a moisturizer and an antioxidant	1148:1199	the potential to be a moisturizer and an antioxidant	1148:1199	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	6	31	contain	have	1143:1146	arg1	fractions					1133:1141	different polysaccharide fractions	1108:1141	different polysaccharide fractions	1108:1141	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	6	32	used	used	1240:1243	arg2	ingredients					1219:1229	their active ingredients	1206:1229	their active ingredients	1206:1229	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	0	33	theme	polysaccharides	70:84	arg1	moisture-preserving					19:37	moisture-preserving	19:37	moisture-preserving	19:37	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	0	33	theme	polysaccharides	70:84	arg1	analysis					9:16	Chemical analysis	0:16	Chemical analysis	0:16	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	0	33	theme	polysaccharides	70:84	arg1	activities					56:65	antioxidant activities	44:65	antioxidant activities	44:65	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	5	34	theme	Monosaccharide	856:869	arg1	analysis					883:890	Monosaccharide composition analysis	856:890	Monosaccharide composition analysis	856:890	Monosaccharide composition analysis shows that P. nameko belongs to heteropolysaccharides, which consists of galactose, glucose, and mannose with different types and ratios, and the molecular weight are distributed at 4.40-333.49kDa.					
30910671	3	35	theme	polysaccharide	466:479	arg1	%					529:529	45.12%	524:529	45.12%	524:529	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	3	35	theme	polysaccharide	466:479	arg1	contents					481:488	the polysaccharide contents	462:488	the polysaccharide contents of PNP-40, PNP-60, and PNP-80	462:518	The results indicate that the polysaccharide contents of PNP-40, PNP-60, and PNP-80 are 45.12%, 78.04%, and 72.22%, respectively, while the β-glucan, protein, and uronic acid contents are 20.20%, 12.20%, and 10.15%, respectively; 11.24%, 14.53%, and 26.94%; and 5.99%, 7.73%, and 3.78%.					
30910671	6	36	theme	ingredients	1276:1286	arg1	development					1252:1262	the development	1248:1262	the development of cosmetic ingredients	1248:1286	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	4	37	theme	better	747:752	arg1	absorption					763:772	better moisture absorption	747:772	better moisture absorption	747:772	Furthermore, PNP-60 has better moisture absorption, while PNP-80 has better antioxidant ability and H2O2-injury resistance activity.					
30910671	0	38	from	moisture-preserving	19:37	arg1	Pholiota					91:98	Pholiota	91:98	Pholiota	91:98	Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation.					
30910671	1	39	theme	nameko	165:170	arg1	PNP-80					230:235	PNP-80	230:235	PNP-80	230:235	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	39	theme	nameko	165:170	arg1	PNP-40					210:215	PNP-40	210:215	PNP-40	210:215	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	39	theme	nameko	165:170	arg1	fractions					199:207	Pholiota nameko (P. nameko) polysaccharide fractions	156:207	Pholiota nameko (P. nameko) polysaccharide fractions	156:207	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	1	39	theme	nameko	165:170	arg1	PNP-60					218:223	PNP-60	218:223	PNP-60	218:223	This study explored Pholiota nameko (P. nameko) polysaccharide fractions, PNP-40, PNP-60, and PNP-80, purified by gradient concentrations of ethanol (40%, 60%, and 80% (v/v)).					
30910671	6	40	theme	cosmetic	1267:1274	arg1	ingredients					1276:1286	cosmetic ingredients	1267:1286	cosmetic ingredients	1267:1286	It was found that different polysaccharide fractions have the potential to be a moisturizer and an antioxidant, and their active ingredients could be used in the development of cosmetic ingredients.					
30910671	5	41	theme	molecular	1038:1046	arg1	weight					1048:1053	the molecular weight	1034:1053	the molecular weight	1034:1053	Monosaccharide composition analysis shows that P. nameko belongs to heteropolysaccharides, which consists of galactose, glucose, and mannose with different types and ratios, and the molecular weight are distributed at 4.40-333.49kDa.					
30914364	2	0	dep	conditions	500:509	arg1	v/v					553:555	v/v	553:555	v/v	553:555	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	pH 4.0					610:615	pH 4.0	610:615	pH 4.0	610:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	w/v					581:583	w/v	581:583	w/v	581:583	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	%					578:578	SC concentration 19%	559:578	SC concentration 19% (w/v)	559:584	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	0	dep	conditions	500:509	arg1	30 °C					599:603	temperature 30 °C	587:603	temperature 30 °C	587:603	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	1	theme	fermentation	519:530	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	1	theme	fermentation	519:530	arg1	v/v					553:555	v/v	553:555	v/v	553:555	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	1	theme	fermentation	519:530	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	6	2	theme	natural	1178:1184	arg1	biomolecules					1186:1197	natural biomolecules	1178:1197	natural biomolecules	1178:1197	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	1	3	theme	three-phase	152:162	arg1	system					183:188	a three-phase partitioning (TPP) system	150:188	a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	150:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	2	4	theme	extraction	421:430	arg1	EY					439:440	EY	439:440	EY	439:440	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	4	theme	extraction	421:430	arg1	yield					432:436	the maximum extraction yield	409:436	the maximum extraction yield (EY) of EPS-D	409:450	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	4	theme	extraction	421:430	arg1	%					461:461	71.02%	456:461	71.02%	456:461	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	5	5	theme	total	1014:1018	arg1	capacity					1032:1039	total antioxidant capacity	1014:1039	total antioxidant capacity	1014:1039	Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T.					
30914364	1	6	theme	Phellinus	371:379	arg1	baumii					381:386	Phellinus baumii	371:386	Phellinus baumii	371:386	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	1	7	theme	partitioning	164:175	arg1	system					183:188	a three-phase partitioning (TPP) system	150:188	a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	150:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	6	8	with	system	1072:1077	arg1	DMC					1084:1086	DMC	1084:1086	DMC	1084:1086	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	1	9	theme	exopolysaccharide	304:320	arg1	partitioning					288:299	partitioning	288:299	partitioning of exopolysaccharide (EPS), namely, EPS-D	288:341	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	2	10	theme	DMC	512:514	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	10	theme	DMC	512:514	arg1	v/v					553:555	v/v	553:555	v/v	553:555	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	10	theme	DMC	512:514	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	1	11	theme	baumii	381:386	arg1	broth					362:366	fermentation broth	349:366	fermentation broth of Phellinus baumii	349:386	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	6	12	theme	TPP	1068:1070	arg1	system					1072:1077	the TPP system	1064:1077	the TPP system with DMC as an alternative solvent for t-butanol	1064:1126	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	1	13	theme	sodium	241:246	arg1	SC					257:258	SC	257:258	SC	257:258	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	1	13	theme	sodium	241:246	arg1	citrate					248:254	sodium citrate	241:254	sodium citrate (SC)	241:259	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	0	14	theme	exopolysaccharides	93:110	arg1	partitioning					77:88	partitioning	77:88	partitioning of exopolysaccharides from Phellinus baumii	77:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	4	15	contain	had	804:806	arg1	EPS-T					798:802	EPS-T	798:802	EPS-T	798:802	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	4	15	contain	had	804:806	arg1	EPS-D					788:792	EPS-D	788:792	EPS-D	788:792	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	4	15	contain	had	804:806	arg2	compositions					827:838	different chemical compositions	808:838	different chemical compositions	808:838	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	4	15	contain	had	804:806	arg2	weights					854:860	molecular weights	844:860	molecular weights	844:860	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	6	16	theme	biomolecules	1186:1197	arg1	partitioning					1162:1173	efficient partitioning	1152:1173	efficient partitioning of natural biomolecules	1152:1197	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	0	17	theme	Phellinus	117:125	arg1	baumii					127:132	Phellinus baumii	117:132	Phellinus baumii	117:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	5	18	theme	free	975:978	arg1	capability					999:1008	free radical-scavenging capability	975:1008	free radical-scavenging capability	975:1008	Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T.					
30914364	6	19	theme	great	1132:1136	arg1	potential					1138:1146	great potential	1132:1146	great potential for efficient partitioning of natural biomolecules	1132:1197	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	3	20	theme	TPP	740:742	arg1	system					744:749	the TPP system	736:749	the TPP system with t-butanol and ammonium sulfate	736:785	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	1	21	theme	TPP	178:180	arg1	system					183:188	a three-phase partitioning (TPP) system	150:188	a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	150:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	2	22	dep	ratio	538:542	arg1	to					516:517	to	516:517	to	516:517	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	5	23	theme	radical-scavenging	980:997	arg1	capability					999:1008	free radical-scavenging capability	975:1008	free radical-scavenging capability	975:1008	Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T.					
30914364	2	24	theme	optimal	492:498	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	24	theme	optimal	492:498	arg1	pH 4.0					610:615	pH 4.0	610:615	pH 4.0	610:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	24	theme	optimal	492:498	arg1	30 °C					599:603	temperature 30 °C	587:603	temperature 30 °C	587:603	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	24	theme	optimal	492:498	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	0	25	theme	Three-phase	0:10	arg1	partitioning					12:23	Three-phase partitioning	0:23	Three-phase partitioning	0:23	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	2	26	theme	broth	532:536	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	26	theme	broth	532:536	arg1	v/v					553:555	v/v	553:555	v/v	553:555	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	26	theme	broth	532:536	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	4	27	theme	chemical	890:897	arg1	structures					899:908	their preliminary chemical structures	872:908	their preliminary chemical structures	872:908	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	3	28	theme	higher	628:633	arg1	contents					669:676	higher EY, carbohydrate, and uronic acid contents	628:676	higher EY, carbohydrate, and uronic acid contents	628:676	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	2	29	theme	concentration	562:574	arg1	w/v					581:583	w/v	581:583	w/v	581:583	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	29	theme	concentration	562:574	arg1	%					578:578	SC concentration 19%	559:578	SC concentration 19% (w/v)	559:584	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	30	theme	SC	559:560	arg1	w/v					581:583	w/v	581:583	w/v	581:583	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	30	theme	SC	559:560	arg1	%					578:578	SC concentration 19%	559:578	SC concentration 19% (w/v)	559:584	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	4	31	theme	preliminary	878:888	arg1	structures					899:908	their preliminary chemical structures	872:908	their preliminary chemical structures	872:908	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	3	32	contain	had	624:626	arg2	contents					669:676	higher EY, carbohydrate, and uronic acid contents	628:676	higher EY, carbohydrate, and uronic acid contents	628:676	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	3	32	contain	had	624:626	arg1	EPS-D					618:622	EPS-D	618:622	EPS-D	618:622	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	1	33	theme	dimethyl	195:202	arg1	DMC					215:217	DMC	215:217	DMC	215:217	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	1	33	theme	dimethyl	195:202	arg1	carbonate					204:212	dimethyl carbonate	195:212	dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	195:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	2	34	theme	maximum	413:419	arg1	EY					439:440	EY	439:440	EY	439:440	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	34	theme	maximum	413:419	arg1	yield					432:436	the maximum extraction yield	409:436	the maximum extraction yield (EY) of EPS-D	409:450	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	34	theme	maximum	413:419	arg1	%					461:461	71.02%	456:461	71.02%	456:461	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	6	35	theme	alternative	1094:1104	arg1	solvent					1106:1112	an alternative solvent	1091:1112	an alternative solvent for t-butanol	1091:1126	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	0	36	from	partitioning	77:88	arg1	baumii					127:132	Phellinus baumii	117:132	Phellinus baumii	117:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	3	37	theme	ammonium	770:777	arg1	sulfate					779:785	ammonium sulfate	770:785	ammonium sulfate	770:785	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	0	38	theme	dimethyl	37:44	arg1	carbonate					46:54	dimethyl carbonate	37:54	dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii	37:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	1	39	theme	salt	264:267	arg1	phase					269:273	salt phase	264:273	salt phase	264:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	4	40	theme	molecular	844:852	arg1	weights					854:860	molecular weights	844:860	molecular weights	844:860	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	2	41	theme	temperature	587:597	arg1	30 °C					599:603	temperature 30 °C	587:603	temperature 30 °C	587:603	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	41	theme	temperature	587:597	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	0	42	theme	organic	59:65	arg1	phase					67:71	organic phase	59:71	organic phase for partitioning of exopolysaccharides from Phellinus baumii	59:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	3	43	theme	carbohydrate	639:650	arg1	contents					669:676	higher EY, carbohydrate, and uronic acid contents	628:676	higher EY, carbohydrate, and uronic acid contents	628:676	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	2	44	theme	EPS-D	446:450	arg1	EY					439:440	EY	439:440	EY	439:440	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	44	theme	EPS-D	446:450	arg1	yield					432:436	the maximum extraction yield	409:436	the maximum extraction yield (EY) of EPS-D	409:450	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	44	theme	EPS-D	446:450	arg1	%					461:461	71.02%	456:461	71.02%	456:461	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	0	45	with	system	25:30	arg1	carbonate					46:54	dimethyl carbonate	37:54	dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii	37:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	5	46	theme	antioxidant	1020:1030	arg1	capacity					1032:1039	total antioxidant capacity	1014:1039	total antioxidant capacity	1014:1039	Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T.					
30914364	3	47	theme	EY	635:636	arg1	contents					669:676	higher EY, carbohydrate, and uronic acid contents	628:676	higher EY, carbohydrate, and uronic acid contents	628:676	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	1	48	with	system	183:188	arg1	DMC					215:217	DMC	215:217	DMC	215:217	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	1	48	with	system	183:188	arg1	carbonate					204:212	dimethyl carbonate	195:212	dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	195:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	0	49	from	baumii	127:132	arg1	partitioning					77:88	partitioning	77:88	partitioning of exopolysaccharides from Phellinus baumii	77:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	0	49	from	baumii	127:132	arg1	exopolysaccharides					93:110	exopolysaccharides	93:110	exopolysaccharides from Phellinus baumii	93:132	Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii.					
30914364	3	50	with	system	744:749	arg1	t-butanol					756:764	t-butanol	756:764	t-butanol	756:764	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	3	50	with	system	744:749	arg1	sulfate					779:785	ammonium sulfate	770:785	ammonium sulfate	770:785	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	6	51	theme	efficient	1152:1160	arg1	partitioning					1162:1173	efficient partitioning	1152:1173	efficient partitioning of natural biomolecules	1152:1197	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	5	52	dep	stronger	966:973	arg1	capability					999:1008	free radical-scavenging capability	975:1008	free radical-scavenging capability	975:1008	Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T.					
30914364	2	53	theme	modified	483:490	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	53	theme	modified	483:490	arg1	pH 4.0					610:615	pH 4.0	610:615	pH 4.0	610:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	53	theme	modified	483:490	arg1	30 °C					599:603	temperature 30 °C	587:603	temperature 30 °C	587:603	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	53	theme	modified	483:490	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	1	54	theme	fermentation	349:360	arg1	broth					362:366	fermentation broth	349:366	fermentation broth of Phellinus baumii	349:386	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	3	55	theme	acid	664:667	arg1	contents					669:676	higher EY, carbohydrate, and uronic acid contents	628:676	higher EY, carbohydrate, and uronic acid contents	628:676	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	2	56	theme	following	473:481	arg1	ratio					538:542	DMC to fermentation broth ratio 0.5:1.0	512:550	DMC to fermentation broth ratio 0.5:1.0 (v/v)	512:556	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	56	theme	following	473:481	arg1	pH 4.0					610:615	pH 4.0	610:615	pH 4.0	610:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	56	theme	following	473:481	arg1	30 °C					599:603	temperature 30 °C	587:603	temperature 30 °C	587:603	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	2	56	theme	following	473:481	arg1	conditions					500:509	the following modified optimal conditions	469:509	the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0	469:615	Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 °C, and pH 4.0.					
30914364	6	57	contain	has	1128:1130	arg1	system					1072:1077	the TPP system	1064:1077	the TPP system with DMC as an alternative solvent for t-butanol	1064:1126	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	6	57	contain	has	1128:1130	arg2	potential					1138:1146	great potential	1132:1146	great potential for efficient partitioning of natural biomolecules	1132:1197	Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.					
30914364	4	58	theme	chemical	818:825	arg1	compositions					827:838	different chemical compositions	808:838	different chemical compositions	808:838	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	4	59	theme	different	808:816	arg1	compositions					827:838	different chemical compositions	808:838	different chemical compositions	808:838	EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged.					
30914364	1	60	used	used	279:282	arg2	system					183:188	a three-phase partitioning (TPP) system	150:188	a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase	150:273	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30914364	3	61	theme	uronic	657:662	arg1	acid					664:667	uronic acid	657:667	uronic acid	657:667	EPS-D had higher EY, carbohydrate, and uronic acid contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate.					
30914364	1	62	theme	organic	223:229	arg1	phase					231:235	organic phase	223:235	organic phase	223:235	In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii.					
30502594	1	0	theme	cell	323:326	arg1	composition					333:343	cell wall composition	323:343	cell wall composition	323:343	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	2	1	with	combination	545:555	arg1	models					569:574	linear models	562:574	linear models	562:574	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	2	theme	prediction	225:234	arg1	models					236:241	accurate and robust prediction models	205:241	accurate and robust prediction models	205:241	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	3	theme	hemicellulose	62:74	arg1	prediction					48:57	the content prediction	36:57	the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues	36:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	1	4	theme	wall	328:331	arg1	composition					333:343	cell wall composition	323:343	cell wall composition	323:343	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	2	5	theme	useful	586:591	arg1	tool					603:606	a useful and rapid tool	584:606	a useful and rapid tool for the determination of cell wall composition of these plant wastes	584:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	2	6	theme	wall	638:641	arg1	composition					643:653	cell wall composition	633:653	cell wall composition of these plant wastes	633:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	7	theme	cellulose	386:394	arg1	determination					354:366	the determination	350:366	the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues	350:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	1	8	theme	models	236:241	arg1	development					190:200	the development	186:200	the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition,	186:344	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	9	from	Use	0:2	arg1	prediction					48:57	the content prediction	36:57	the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues	36:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	2	10	theme	cell	633:636	arg1	composition					643:653	cell wall composition	633:653	cell wall composition of these plant wastes	633:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	5	11	theme	predicted	1113:1121	arg1	values					1123:1128	our predicted values	1109:1128	our predicted values	1109:1128	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	0	12	theme	cellulose	77:85	arg1	prediction					48:57	the content prediction	36:57	the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues	36:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	3	13	theme	cellulose	815:823	arg1	determinations					825:838	cellulose determinations	815:838	cellulose determinations	815:838	Regularized linear regression methods have also been applied to avoid overfitting, producing improved models specifically for lignin and cellulose determinations.					
30502594	4	14	theme	predictive	845:854	arg1	models					856:861	The predictive models	841:861	The predictive models	841:861	The predictive models are also presented in a desktop application available at http://www2.ual.es/NMRMBC/solutions.					
30502594	1	15	theme	greenhouse	432:441	arg1	residues					448:455	greenhouse crop residues	432:455	greenhouse crop residues	432:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	4	16	from	http	920:923	arg1	available					907:915	available	907:915	available	907:915	The predictive models are also presented in a desktop application available at http://www2.ual.es/NMRMBC/solutions.					
30502594	0	17	theme	lignin	91:96	arg1	prediction					48:57	the content prediction	36:57	the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues	36:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	3	18	theme	regression	697:706	arg1	methods					708:714	Regularized linear regression methods	678:714	Regularized linear regression methods	678:714	Regularized linear regression methods have also been applied to avoid overfitting, producing improved models specifically for lignin and cellulose determinations.					
30502594	1	19	theme	crop	443:446	arg1	residues					448:455	greenhouse crop residues	432:455	greenhouse crop residues	432:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	2	20	theme	present	462:468	arg1	paper					470:474	The present paper	458:474	The present paper	458:474	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	21	theme	residues	448:455	arg1	species					421:427	8 species	419:427	8 species of greenhouse crop residues	419:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	4	22	theme	desktop	887:893	arg1	application					895:905	a desktop application	885:905	a desktop application available at http://www2.ual.es/NMRMBC/solutions	885:954	The predictive models are also presented in a desktop application available at http://www2.ual.es/NMRMBC/solutions.					
30502594	1	23	theme	multivariate	157:168	arg1	analysis					174:181	multivariate NMR analysis	157:181	multivariate NMR analysis	157:181	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	1	24	from	determination	354:366	arg1	species					421:427	8 species	419:427	8 species of greenhouse crop residues	419:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	2	25	theme	rapid	597:601	arg1	tool					603:606	a useful and rapid tool	584:606	a useful and rapid tool for the determination of cell wall composition of these plant wastes	584:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	3	26	theme	improved	771:778	arg1	models					780:785	improved models	771:785	improved models	771:785	Regularized linear regression methods have also been applied to avoid overfitting, producing improved models specifically for lignin and cellulose determinations.					
30502594	1	27	theme	NMR	170:172	arg1	analysis					174:181	multivariate NMR analysis	157:181	multivariate NMR analysis	157:181	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	28	theme	NMR	20:22	arg1	analysis					24:31	multivariate NMR analysis	7:31	multivariate NMR analysis	7:31	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	0	29	theme	crop	112:115	arg1	residues					117:124	greenhouse crop residues	101:124	greenhouse crop residues	101:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	2	30	theme	composition	643:653	arg1	determination					616:628	the determination	612:628	the determination of cell wall composition of these plant wastes	612:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	31	theme	analysis	174:181	arg1	use					150:152	the use	146:152	the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition,	146:344	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	32	theme	multivariate	7:18	arg1	analysis					24:31	multivariate NMR analysis	7:31	multivariate NMR analysis	7:31	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	0	33	theme	greenhouse	101:110	arg1	residues					117:124	greenhouse crop residues	101:124	greenhouse crop residues	101:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	1	34	from	species	421:427	arg1	determination					354:366	the determination	350:366	the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues	350:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	4	35	dep	http	920:923	arg1	//www2.ual.es/NMRMBC/solutions					925:954	//www2.ual.es/NMRMBC/solutions	925:954	http://www2.ual.es/NMRMBC/solutions	920:954	The predictive models are also presented in a desktop application available at http://www2.ual.es/NMRMBC/solutions.					
30502594	2	36	theme	PLS-DA	529:534	arg1	model					536:540	a PLS-DA model	527:540	a PLS-DA model	527:540	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	37	theme	hemicellulose	371:383	arg1	determination					354:366	the determination	350:366	the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues	350:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	38	theme	analysis	24:31	arg1	Use					0:2	Use	0:2	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.	0:125	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	1	39	from	hemicellulose	371:383	arg1	species					421:427	8 species	419:427	8 species of greenhouse crop residues	419:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	40	theme	content	40:46	arg1	prediction					48:57	the content prediction	36:57	the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues	36:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	1	41	theme	soluble	291:297	arg1	profiles					310:317	soluble metabolite profiles	291:317	soluble metabolite profiles	291:317	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	0	42	from	prediction	48:57	arg1	residues					117:124	greenhouse crop residues	101:124	greenhouse crop residues	101:124	Use of multivariate NMR analysis in the content prediction of hemicellulose, cellulose and lignin in greenhouse crop residues.					
30502594	5	43	theme	accepted	1054:1061	arg1	protocols					1063:1071	generally accepted protocols	1044:1071	generally accepted protocols	1044:1071	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	3	44	theme	linear	690:695	arg1	methods					708:714	Regularized linear regression methods	678:714	Regularized linear regression methods	678:714	Regularized linear regression methods have also been applied to avoid overfitting, producing improved models specifically for lignin and cellulose determinations.					
30502594	1	45	theme	metabolite	299:308	arg1	profiles					310:317	soluble metabolite profiles	291:317	soluble metabolite profiles	291:317	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	2	46	theme	linear	562:567	arg1	models					569:574	linear models	562:574	linear models	562:574	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	1	47	from	cellulose	386:394	arg1	species					421:427	8 species	419:427	8 species of greenhouse crop residues	419:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	1	48	theme	lignin	400:405	arg1	contents					407:414	lignin contents	400:414	lignin contents in 8 species of greenhouse crop residues	400:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	5	49	theme	models	1006:1011	arg1	reliability					987:997	reliability	987:997	reliability	987:997	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	5	49	theme	models	1006:1011	arg1	rationality					971:981	rationality	971:981	rationality	971:981	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	2	50	theme	wastes	670:675	arg1	composition					643:653	cell wall composition	633:653	cell wall composition of these plant wastes	633:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	2	51	theme	discriminant	494:505	arg1	buckets					507:513	discriminant buckets	494:513	discriminant buckets coming from a PLS-DA model in combination with linear models	494:574	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	2	52	theme	plant	664:668	arg1	wastes					670:675	these plant wastes	658:675	these plant wastes	658:675	The present paper demonstrates that discriminant buckets coming from a PLS-DA model in combination with linear models provide a useful and rapid tool for the determination of cell wall composition of these plant wastes.					
30502594	4	53	theme	available	907:915	arg1	application					895:905	a desktop application	885:905	a desktop application available at http://www2.ual.es/NMRMBC/solutions	885:954	The predictive models are also presented in a desktop application available at http://www2.ual.es/NMRMBC/solutions.					
30502594	1	54	theme	contents	407:414	arg1	determination					354:366	the determination	350:366	the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues	350:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	1	55	theme	accurate	205:212	arg1	models					236:241	accurate and robust prediction models	205:241	accurate and robust prediction models	205:241	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	1	56	from	contents	407:414	arg1	species					421:427	8 species	419:427	8 species of greenhouse crop residues	419:455	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	5	57	theme	control	1014:1020	arg1	experiments					1022:1032	control experiments	1014:1032	control experiments following generally accepted protocols	1014:1071	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	3	58	theme	Regularized	678:688	arg1	methods					708:714	Regularized linear regression methods	678:714	Regularized linear regression methods	678:714	Regularized linear regression methods have also been applied to avoid overfitting, producing improved models specifically for lignin and cellulose determinations.					
30502594	1	59	from	use	150:152	arg1	development					190:200	the development	186:200	the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition,	186:344	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
30502594	5	60	dep	rationality	971:981	arg1	the					967:969	the	967:969	the	967:969	To verify the rationality and reliability of the models, control experiments following generally accepted protocols have been performed and compared to our predicted values.					
30502594	1	61	theme	robust	218:223	arg1	models					236:241	accurate and robust prediction models	205:241	accurate and robust prediction models	205:241	We have introduced the use of multivariate NMR analysis in the development of accurate and robust prediction models, potentially arising from a correlation between soluble metabolite profiles and cell wall composition, for the determination of hemicellulose, cellulose and lignin contents in 8 species of greenhouse crop residues.					
29425872	8	0	theme	different	1322:1330	arg1	PSDPs					1332:1336	the four different PSDPs	1313:1336	the four different PSDPs modified by the same chemical method	1313:1373	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	1	1	dep	in	319:320	arg1	antioxidant					328:338	antioxidant	328:338	antioxidant	328:338	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	1	1	dep	in	319:320	arg1	vitro					322:326	vitro	322:326	vitro	322:326	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	5	2	theme	reducing	881:888	arg1	ability					890:896	the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)]	869:1019	ability	890:896	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	1	3	theme	extracted	165:173	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	6	4	dep	ability	1166:1172	arg1	%					1180:1180	99.94%	1175:1180	99.94% for all the fractions	1175:1202	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	0	5	theme	polysaccharides	77:91	arg1	activities					63:72	the antioxidant activities	47:72	the antioxidant activities of polysaccharides sequentially extracted from peony	47:125	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	5	6	theme	scavenging	929:938	arg1	ability					940:946	DPPH radical scavenging ability [S-CASS	916:954	DPPH radical scavenging ability [S-CASS (95.07%)]	916:964	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	6	theme	scavenging	929:938	arg1	%					962:962	95.07%	957:962	95.07%	957:962	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	1	7	theme	peony	175:179	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	9	8	theme	different	1507:1515	arg1	modifications					1517:1529	the different modifications	1503:1529	the different modifications of the polysaccharide fractions	1503:1561	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	9	theme	food	1624:1627	arg1	industry					1629:1636	food industry	1624:1636	food industry	1624:1636	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	0	10	from	Effects	0:6	arg1	activities					63:72	the antioxidant activities	47:72	the antioxidant activities of polysaccharides sequentially extracted from peony	47:125	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	5	11	theme	radical	921:927	arg1	ability					940:946	DPPH radical scavenging ability [S-CASS	916:954	DPPH radical scavenging ability [S-CASS (95.07%)]	916:964	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	11	theme	radical	921:927	arg1	%					962:962	95.07%	957:962	95.07%	957:962	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	4	12	theme	POH	795:797	arg1	bonds					799:803	OS, COO and POH bonds	783:803	OS, COO and POH bonds	783:803	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	4	13	theme	infrared	651:658	arg1	spectra					667:673	infrared (FTIR) spectra	651:673	infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives	651:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	9	14	theme	new	1603:1605	arg1	significance					1587:1598	their own potential significance	1567:1598	their own potential significance	1567:1598	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	14	theme	new	1603:1605	arg1	antioxidants					1607:1618	new antioxidants	1603:1618	new antioxidants for food industry and human health	1603:1653	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	6	15	theme	ion	1152:1154	arg1	ability					1166:1172	ferrous ion chelating ability	1144:1172	ferrous ion chelating ability (99.94% for all the fractions)	1144:1203	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	5	16	theme	scavenging	983:992	arg1	%					1017:1017	99.85%	1012:1017	99.85%	1012:1017	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	16	theme	scavenging	983:992	arg1	activity					994:1001	ABTS radical scavenging activity [S-CASS	970:1009	ABTS radical scavenging activity [S-CASS (99.85%)]	970:1019	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	2	17	theme	sugar	399:403	arg1	content					417:423	sugar and protein content	399:423	content	417:423	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	7	18	theme	stable	1276:1281	arg1	ability					1295:1301	moderately stable antioxidant ability	1265:1301	moderately stable antioxidant ability	1265:1301	The carboxymethylated polysaccharides (C-PSDPs) maintained moderately stable antioxidant ability.					
29425872	6	19	theme	radical	1094:1100	arg1	%					1136:1136	92.61%	1131:1136	92.61%	1131:1136	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	19	theme	radical	1094:1100	arg1	activity					1113:1120	maximum hydroxyl radical scavenging activity [P-DASS	1077:1128	maximum hydroxyl radical scavenging activity [P-DASS (92.61%)]	1077:1138	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	3	20	theme	maximum	567:573	arg1	peaks					586:590	the similar maximum absorption peaks	555:590	the similar maximum absorption peaks	555:590	Further, modified derivatives shared the similar maximum absorption peaks indicating their homogeneous nature.					
29425872	6	21	theme	maximum	1077:1083	arg1	%					1136:1136	92.61%	1131:1136	92.61%	1131:1136	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	21	theme	maximum	1077:1083	arg1	activity					1113:1120	maximum hydroxyl radical scavenging activity [P-DASS	1077:1128	maximum hydroxyl radical scavenging activity [P-DASS (92.61%)]	1077:1138	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	1	22	theme	seed	181:184	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	9	23	theme	polysaccharide	1538:1551	arg1	fractions					1553:1561	the polysaccharide fractions	1534:1561	the polysaccharide fractions	1534:1561	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	3	24	theme	absorption	575:584	arg1	peaks					586:590	the similar maximum absorption peaks	555:590	the similar maximum absorption peaks	555:590	Further, modified derivatives shared the similar maximum absorption peaks indicating their homogeneous nature.					
29425872	6	25	theme	scavenging	1102:1111	arg1	%					1136:1136	92.61%	1131:1136	92.61%	1131:1136	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	25	theme	scavenging	1102:1111	arg1	activity					1113:1120	maximum hydroxyl radical scavenging activity [P-DASS	1077:1128	maximum hydroxyl radical scavenging activity [P-DASS (92.61%)]	1077:1138	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	1	26	theme	dreg	186:189	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	2	27	theme	native	480:485	arg1	polysaccharides					501:515	native (un-modified) polysaccharides	480:515	native (un-modified) polysaccharides	480:515	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	2	28	dep	native	480:485	arg1	un-modified					488:498	un-modified	488:498	un-modified	488:498	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	6	29	theme	chelating	1156:1164	arg1	ability					1166:1172	ferrous ion chelating ability	1144:1172	ferrous ion chelating ability (99.94% for all the fractions)	1144:1203	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	1	30	theme	polysaccharides	191:205	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	6	31	theme	phosphorylated	1026:1039	arg1	P-PSDPs					1058:1064	P-PSDPs	1058:1064	P-PSDPs	1058:1064	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	31	theme	phosphorylated	1026:1039	arg1	polysaccharides					1041:1055	The phosphorylated polysaccharides	1022:1055	The phosphorylated polysaccharides (P-PSDPs)	1022:1065	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	4	32	theme	phosphorylated	710:723	arg1	derivatives					725:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	4	33	theme	COO	787:789	arg1	bonds					799:803	OS, COO and POH bonds	783:803	OS, COO and POH bonds	783:803	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	4	34	theme	derivatives	725:735	arg1	spectra					667:673	infrared (FTIR) spectra	651:673	infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives	651:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	0	35	theme	chemical	21:28	arg1	modifications					30:42	different chemical modifications	11:42	different chemical modifications	11:42	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	8	36	theme	chemical	1404:1411	arg1	composition					1413:1423	different chemical composition	1394:1423	different chemical composition	1394:1423	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	2	37	dep	showed	382:387	arg1	decreased					389:397	decreased	389:397	decreased	389:397	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	5	38	theme	ABTS	970:973	arg1	%					1017:1017	99.85%	1012:1017	99.85%	1012:1017	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	38	theme	ABTS	970:973	arg1	activity					994:1001	ABTS radical scavenging activity [S-CASS	970:1009	ABTS radical scavenging activity [S-CASS (99.85%)]	970:1019	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	1	39	theme	PSDPs	208:212	arg1	fractions					215:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	152:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	0	40	theme	different	11:19	arg1	modifications					30:42	different chemical modifications	11:42	different chemical modifications	11:42	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	8	41	theme	same	1354:1357	arg1	method					1368:1373	the same chemical method	1350:1373	the same chemical method	1350:1373	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	8	42	theme	antioxidant	1462:1472	arg1	attributes					1474:1483	antioxidant attributes	1462:1483	antioxidant attributes	1462:1483	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	3	43	theme	homogeneous	609:619	arg1	nature					621:626	their homogeneous nature	603:626	their homogeneous nature	603:626	Further, modified derivatives shared the similar maximum absorption peaks indicating their homogeneous nature.					
29425872	4	44	theme	hydroxyl	749:756	arg1	groups					758:763	hydroxyl groups	749:763	hydroxyl groups	749:763	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	5	45	theme	sulfated	824:831	arg1	S-PSDPs					850:856	S-PSDPs	850:856	S-PSDPs	850:856	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	45	theme	sulfated	824:831	arg1	polysaccharides					833:847	The sulfated polysaccharides	820:847	The sulfated polysaccharides (S-PSDPs)	820:857	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	1	46	theme	in	319:320	arg1	characteristics					340:354	their in vitro antioxidant characteristics	313:354	their in vitro antioxidant characteristics	313:354	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	4	47	theme	carboxymethylated	688:704	arg1	derivatives					725:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	8	48	theme	different	1394:1402	arg1	composition					1413:1423	different chemical composition	1394:1423	different chemical composition	1394:1423	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	1	49	theme	fractions	215:223	arg1	types					143:147	Four types	138:147	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions	138:223	Four types of sequentially extracted peony seed dreg polysaccharides (PSDPs) fractions were modified through sulfation, phosphorylation and carboxymethylation and studied for their in vitro antioxidant characteristics.					
29425872	0	50	theme	modifications	30:42	arg1	Effects					0:6	Effects	0:6	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony	0:125	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	4	51	dep	Fourier	633:639	arg1	transform					641:649	transform	641:649	transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives	641:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	2	52	theme	acid	439:442	arg1	content					444:450	uronic acid content	432:450	uronic acid content	432:450	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	0	53	theme	antioxidant	51:61	arg1	activities					63:72	the antioxidant activities	47:72	the antioxidant activities of polysaccharides sequentially extracted from peony	47:125	Effects of different chemical modifications on the antioxidant activities of polysaccharides sequentially extracted from peony seed dreg.					
29425872	5	54	theme	DPPH	916:919	arg1	ability					940:946	DPPH radical scavenging ability [S-CASS	916:954	DPPH radical scavenging ability [S-CASS (95.07%)]	916:964	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	54	theme	DPPH	916:919	arg1	%					962:962	95.07%	957:962	95.07%	957:962	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	9	55	theme	human	1642:1646	arg1	health					1648:1653	human health	1642:1653	human health	1642:1653	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	8	56	theme	chemical	1359:1366	arg1	method					1368:1373	the same chemical method	1350:1373	the same chemical method	1350:1373	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	5	57	theme	[S-CASS	948:954	arg1	ability					940:946	DPPH radical scavenging ability [S-CASS	916:954	DPPH radical scavenging ability [S-CASS (95.07%)]	916:964	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	57	theme	[S-CASS	948:954	arg1	%					962:962	95.07%	957:962	95.07%	957:962	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	2	58	theme	uronic	432:437	arg1	content					444:450	uronic acid content	432:450	uronic acid content	432:450	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	9	59	contain	had	1563:1565	arg2	antioxidants					1607:1618	new antioxidants	1603:1618	new antioxidants for food industry and human health	1603:1653	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	59	contain	had	1563:1565	arg1	modifications					1517:1529	the different modifications	1503:1529	the different modifications of the polysaccharide fractions	1503:1561	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	59	contain	had	1563:1565	arg2	significance					1587:1598	their own potential significance	1567:1598	their own potential significance	1567:1598	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	60	theme	own	1573:1575	arg1	significance					1587:1598	their own potential significance	1567:1598	their own potential significance	1567:1598	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	60	theme	own	1573:1575	arg1	antioxidants					1607:1618	new antioxidants	1603:1618	new antioxidants for food industry and human health	1603:1653	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	8	61	theme	absorption	1441:1450	arg1	peaks					1452:1456	characteristic absorption peaks	1426:1456	characteristic absorption peaks	1426:1456	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	3	62	theme	modified	527:534	arg1	derivatives					536:546	modified derivatives	527:546	modified derivatives	527:546	Further, modified derivatives shared the similar maximum absorption peaks indicating their homogeneous nature.					
29425872	5	63	dep	ability	890:896	arg1	[S-CASS					898:904	[S-CASS	898:904	[S-CASS (1.854)]	898:913	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	63	dep	ability	890:896	arg1	1.854					907:911	1.854	907:911	1.854	907:911	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	8	64	theme	characteristic	1426:1439	arg1	peaks					1452:1456	characteristic absorption peaks	1426:1456	characteristic absorption peaks	1426:1456	Overall, the four different PSDPs modified by the same chemical method also resulted into different chemical composition, characteristic absorption peaks and antioxidant attributes.					
29425872	9	65	theme	fractions	1553:1561	arg1	modifications					1517:1529	the different modifications	1503:1529	the different modifications of the polysaccharide fractions	1503:1561	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	2	66	theme	modified	361:368	arg1	derivatives					370:380	The modified derivatives	357:380	The modified derivatives	357:380	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	6	67	theme	ferrous	1144:1150	arg1	ability					1166:1172	ferrous ion chelating ability	1144:1172	ferrous ion chelating ability (99.94% for all the fractions)	1144:1203	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	4	68	theme	FTIR	661:664	arg1	spectra					667:673	infrared (FTIR) spectra	651:673	infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives	651:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	5	69	theme	[S-CASS	1003:1009	arg1	%					1017:1017	99.85%	1012:1017	99.85%	1012:1017	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	69	theme	[S-CASS	1003:1009	arg1	activity					994:1001	ABTS radical scavenging activity [S-CASS	970:1009	ABTS radical scavenging activity [S-CASS (99.85%)]	970:1019	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	7	70	theme	antioxidant	1283:1293	arg1	ability					1295:1301	moderately stable antioxidant ability	1265:1301	moderately stable antioxidant ability	1265:1301	The carboxymethylated polysaccharides (C-PSDPs) maintained moderately stable antioxidant ability.					
29425872	5	71	theme	radical	975:981	arg1	%					1017:1017	99.85%	1012:1017	99.85%	1012:1017	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	5	71	theme	radical	975:981	arg1	activity					994:1001	ABTS radical scavenging activity [S-CASS	970:1009	ABTS radical scavenging activity [S-CASS (99.85%)]	970:1019	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	4	72	theme	OS	783:784	arg1	bonds					799:803	OS, COO and POH bonds	783:803	OS, COO and POH bonds	783:803	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	6	73	theme	[P-DASS	1122:1128	arg1	%					1136:1136	92.61%	1131:1136	92.61%	1131:1136	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	73	theme	[P-DASS	1122:1128	arg1	activity					1113:1120	maximum hydroxyl radical scavenging activity [P-DASS	1077:1128	maximum hydroxyl radical scavenging activity [P-DASS (92.61%)]	1077:1138	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	4	74	theme	sulfated	678:685	arg1	derivatives					725:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	sulfated, carboxymethylated and phosphorylated derivatives	678:735	The Fourier transform infrared (FTIR) spectra of sulfated, carboxymethylated and phosphorylated derivatives showed that hydroxyl groups were converted to OS, COO and POH bonds, respectively.					
29425872	2	75	theme	protein	409:415	arg1	content					417:423	sugar and protein content	399:423	content	417:423	The modified derivatives showed decreased sugar and protein content, while uronic acid content was elevated as compared to native (un-modified) polysaccharides.					
29425872	6	76	theme	hydroxyl	1085:1092	arg1	%					1136:1136	92.61%	1131:1136	92.61%	1131:1136	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	6	76	theme	hydroxyl	1085:1092	arg1	activity					1113:1120	maximum hydroxyl radical scavenging activity [P-DASS	1077:1128	maximum hydroxyl radical scavenging activity [P-DASS (92.61%)]	1077:1138	The phosphorylated polysaccharides (P-PSDPs) exhibited maximum hydroxyl radical scavenging activity [P-DASS (92.61%)] and ferrous ion chelating ability (99.94% for all the fractions).					
29425872	9	77	theme	potential	1577:1585	arg1	significance					1587:1598	their own potential significance	1567:1598	their own potential significance	1567:1598	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	9	77	theme	potential	1577:1585	arg1	antioxidants					1607:1618	new antioxidants	1603:1618	new antioxidants for food industry and human health	1603:1653	We conclude that the different modifications of the polysaccharide fractions had their own potential significance as new antioxidants for food industry and human health.					
29425872	7	78	theme	carboxymethylated	1210:1226	arg1	C-PSDPs					1245:1251	C-PSDPs	1245:1251	C-PSDPs	1245:1251	The carboxymethylated polysaccharides (C-PSDPs) maintained moderately stable antioxidant ability.					
29425872	7	78	theme	carboxymethylated	1210:1226	arg1	polysaccharides					1228:1242	The carboxymethylated polysaccharides	1206:1242	The carboxymethylated polysaccharides (C-PSDPs)	1206:1252	The carboxymethylated polysaccharides (C-PSDPs) maintained moderately stable antioxidant ability.					
29425872	5	79	theme	highest	873:879	arg1	ability					890:896	the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)]	869:1019	ability	890:896	The sulfated polysaccharides (S-PSDPs) displayed the highest reducing ability [S-CASS (1.854)], DPPH radical scavenging ability [S-CASS (95.07%)] and ABTS radical scavenging activity [S-CASS (99.85%)].					
29425872	3	80	theme	similar	559:565	arg1	peaks					586:590	the similar maximum absorption peaks	555:590	the similar maximum absorption peaks	555:590	Further, modified derivatives shared the similar maximum absorption peaks indicating their homogeneous nature.					
31843606	7	0	theme	starch-urea-melamine	1033:1052	arg1	composites					1054:1063	starch-urea-melamine composites	1033:1063	starch-urea-melamine composites	1033:1063	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	6	1	dep	techniques	776:785	arg1	FT-Raman					788:795	FT-Raman	788:795	FT-Raman	788:795	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	6	1	dep	techniques	776:785	arg1	NMR					805:807	13C NMR	801:807	13C NMR	801:807	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	6	1	dep	techniques	776:785	arg1	techniques					776:785	spectroscopic techniques	762:785	spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch	762:869	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	1	2	theme	controlled-release	212:229	arg1	fertilizer					237:246	controlled-release plant fertilizer	212:246	controlled-release plant fertilizer	212:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	4	3	theme	nitrogen	492:499	arg1	content					501:507	high nitrogen content	487:507	high nitrogen content	487:507	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	7	4	theme	plasticizing	1010:1021	arg1	effect					1023:1028	an enhanced starch plasticizing effect	991:1028	an enhanced starch plasticizing effect of starch-urea-melamine composites	991:1063	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	4	5	contain	has	483:485	arg2	content					501:507	high nitrogen content	487:507	high nitrogen content	487:507	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	4	5	contain	has	483:485	arg1	Melamine					469:476	Melamine	469:476	Melamine	469:476	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	1	6	theme	plant	231:235	arg1	fertilizer					237:246	controlled-release plant fertilizer	212:246	controlled-release plant fertilizer	212:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	4	7	theme	high	487:490	arg1	content					501:507	high nitrogen content	487:507	high nitrogen content	487:507	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	5	8	theme	performance	664:674	arg1	gain					676:679	the performance gain	660:679	the performance gain of melamine addition to starch-urea composites	660:726	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	4	9	theme	controlled-release	579:596	arg1	fertilizer					598:607	enhanced controlled-release fertilizer	570:607	enhanced controlled-release fertilizer	570:607	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	0	10	theme	fertilizer	80:89	arg1	application					91:101	fertilizer application	80:101	fertilizer application	80:101	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.					
31843606	8	11	theme	starch	1113:1118	arg1	co-plasticization					1092:1108	the co-plasticization	1088:1108	the co-plasticization of starch by urea and melamine	1088:1139	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	7	12	theme	enhanced	994:1001	arg1	effect					1023:1028	an enhanced starch plasticizing effect	991:1028	an enhanced starch plasticizing effect of starch-urea-melamine composites	991:1063	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	4	13	theme	enhanced	570:577	arg1	fertilizer					598:607	enhanced controlled-release fertilizer	570:607	enhanced controlled-release fertilizer	570:607	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	8	14	theme	Further	1066:1072	arg1	research					1074:1081	Further research	1066:1081	Further research into the co-plasticization of starch by urea and melamine	1066:1139	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	7	15	theme	starch	1003:1008	arg1	effect					1023:1028	an enhanced starch plasticizing effect	991:1028	an enhanced starch plasticizing effect of starch-urea-melamine composites	991:1063	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	0	16	theme	synergic	29:36	arg1	co-plasticizers					38:52	synergic co-plasticizers	29:52	synergic co-plasticizers for starch composites for fertilizer application	29:101	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.					
31843606	2	17	theme	Slow-release	249:260	arg1	fertilizers					262:272	Slow-release fertilizers	249:272	Slow-release fertilizers	249:272	Slow-release fertilizers are important in minimizing nutrient losses due to run-off, leaching, and other factors.					
31843606	5	18	theme	structural	633:642	arg1	interaction					644:654	the structural interaction	629:654	the structural interaction	629:654	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	1	19	from	interaction	127:137	arg1	materials					191:199	composite materials	181:199	composite materials for use as controlled-release plant fertilizer	181:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	8	20	theme	controlled-release	1173:1190	arg1	products					1203:1210	improved controlled-release fertilizer products	1164:1210	improved controlled-release fertilizer products	1164:1210	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	1	21	theme	melamine	160:167	arg1	interaction					127:137	the interaction	123:137	the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer	123:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	7	22	theme	processing	956:965	arg1	temperatures					967:978	processing temperatures	956:978	processing temperatures	956:978	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	2	23	theme	nutrient	302:309	arg1	losses					311:316	nutrient losses	302:316	nutrient losses due to run-off, leaching, and other factors	302:360	Slow-release fertilizers are important in minimizing nutrient losses due to run-off, leaching, and other factors.					
31843606	5	24	theme	starch-urea	705:715	arg1	composites					717:726	starch-urea composites	705:726	starch-urea composites	705:726	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	6	25	theme	13C	801:803	arg1	NMR					805:807	13C NMR	801:807	13C NMR	801:807	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	6	25	theme	13C	801:803	arg1	techniques					776:785	spectroscopic techniques	762:785	spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch	762:869	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	0	26	theme	urea	8:11	arg1	Role					0:3	Role	0:3	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.	0:102	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.					
31843606	9	27	theme	controlled-release	1320:1337	arg1	products					1350:1357	improved controlled-release fertilizer products	1311:1357	improved controlled-release fertilizer products	1311:1357	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	1	28	theme	urea	150:153	arg1	interaction					127:137	the interaction	123:137	the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer	123:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	4	29	theme	urea-starch	536:546	arg1	composites					548:557	urea-starch composites	536:557	urea-starch composites	536:557	Melamine also has high nitrogen content and could be combined with urea-starch composites to provide enhanced controlled-release fertilizer.					
31843606	8	30	theme	improved	1164:1171	arg1	products					1203:1210	improved controlled-release fertilizer products	1164:1210	improved controlled-release fertilizer products	1164:1210	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	9	31	theme	fertilizer	1339:1348	arg1	products					1350:1357	improved controlled-release fertilizer products	1311:1357	improved controlled-release fertilizer products	1311:1357	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	3	32	theme	important	417:425	arg1	fertilizer					436:445	an important nitrogen fertilizer	414:445	an important nitrogen fertilizer throughout the world	414:466	Urea is an effective plasticizer for starch and is an important nitrogen fertilizer throughout the world.					
31843606	0	33	theme	melamine	17:24	arg1	Role					0:3	Role	0:3	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.	0:102	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.					
31843606	9	34	theme	Further	1213:1219	arg1	research					1221:1228	Further research	1213:1228	Further research into the co-plasticization of starch by urea and melamine	1213:1286	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	3	35	theme	nitrogen	427:434	arg1	fertilizer					436:445	an important nitrogen fertilizer	414:445	an important nitrogen fertilizer throughout the world	414:466	Urea is an effective plasticizer for starch and is an important nitrogen fertilizer throughout the world.					
31843606	8	36	theme	fertilizer	1192:1201	arg1	products					1203:1210	improved controlled-release fertilizer products	1164:1210	improved controlled-release fertilizer products	1164:1210	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	3	37	theme	effective	374:382	arg1	Urea					363:366	Urea	363:366	Urea	363:366	Urea is an effective plasticizer for starch and is an important nitrogen fertilizer throughout the world.					
31843606	3	37	theme	effective	374:382	arg1	plasticizer					384:394	an effective plasticizer	371:394	an effective plasticizer for starch	371:405	Urea is an effective plasticizer for starch and is an important nitrogen fertilizer throughout the world.					
31843606	5	38	theme	melamine	684:691	arg1	addition					693:700	melamine addition	684:700	melamine addition to starch-urea composites	684:726	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	9	39	theme	improved	1311:1318	arg1	products					1350:1357	improved controlled-release fertilizer products	1311:1357	improved controlled-release fertilizer products	1311:1357	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	5	40	theme	addition	693:700	arg1	interaction					644:654	the structural interaction	629:654	the structural interaction	629:654	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	5	40	theme	addition	693:700	arg1	gain					676:679	the performance gain	660:679	the performance gain of melamine addition to starch-urea composites	660:726	This study reports the structural interaction and the performance gain of melamine addition to starch-urea composites.					
31843606	7	41	theme	composites	1054:1063	arg1	effect					1023:1028	an enhanced starch plasticizing effect	991:1028	an enhanced starch plasticizing effect of starch-urea-melamine composites	991:1063	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31843606	6	42	theme	spectroscopic	762:774	arg1	FT-Raman					788:795	FT-Raman	788:795	FT-Raman	788:795	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	6	42	theme	spectroscopic	762:774	arg1	NMR					805:807	13C NMR	801:807	13C NMR	801:807	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	6	42	theme	spectroscopic	762:774	arg1	techniques					776:785	spectroscopic techniques	762:785	spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch	762:869	Composites were characterized by spectroscopic techniques (FT-Raman and 13C NMR) detailing the interaction between melamine, urea, and starch.					
31843606	2	43	theme	due	318:320	arg1	losses					311:316	nutrient losses	302:316	nutrient losses due to run-off, leaching, and other factors	302:360	Slow-release fertilizers are important in minimizing nutrient losses due to run-off, leaching, and other factors.					
31843606	0	44	theme	starch	58:63	arg1	composites					65:74	starch composites	58:74	starch composites for fertilizer application	58:101	Role of urea and melamine as synergic co-plasticizers for starch composites for fertilizer application.					
31843606	1	45	theme	composite	181:189	arg1	materials					191:199	composite materials	181:199	composite materials for use as controlled-release plant fertilizer	181:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	2	46	theme	other	348:352	arg1	factors					354:360	other factors	348:360	other factors	348:360	Slow-release fertilizers are important in minimizing nutrient losses due to run-off, leaching, and other factors.					
31843606	9	47	theme	starch	1260:1265	arg1	co-plasticization					1239:1255	the co-plasticization	1235:1255	the co-plasticization of starch by urea and melamine	1235:1286	Further research into the co-plasticization of starch by urea and melamine could be exploited for improved controlled-release fertilizer products.					
31843606	1	48	theme	starch	142:147	arg1	interaction					127:137	the interaction	123:137	the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer	123:246	Herein we describe the interaction of starch, urea, and melamine (C3N6H6) in composite materials for use as controlled-release plant fertilizer.					
31843606	7	49	theme	extrusion	909:917	arg1	processing					919:928	extrusion processing	909:928	extrusion processing	909:928	These interactions helped facilitate extrusion processing by lowering viscosity and processing temperatures suggesting an enhanced starch plasticizing effect of starch-urea-melamine composites.					
31730973	0	0	theme	biodegradation	59:72	arg1	properties					74:83	the thermal, mechanical and biodegradation properties	31:83	the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite	31:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	4	1	theme	fundamental	566:576	arg1	properties					578:587	the fundamental properties	562:587	the fundamental properties of CFG/TPCS biopolymer composites	562:621	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	8	2	theme	burial	1086:1091	arg1	test					1093:1096	Soil burial test	1081:1096	Soil burial test	1081:1096	Soil burial test shows that incorporation of CGF into TPCS has slow down the biodegradation process of the composites.					
31730973	4	3	theme	biodegradable	682:694	arg1	reinforcement					696:708	a biodegradable reinforcement	680:708	a biodegradable reinforcement	680:708	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	4	3	theme	biodegradable	682:694	arg1	potential					667:675	their potential	661:675	their potential	661:675	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	1	4	theme	due	260:262	arg1	polymer					252:258	the non-biodegradable petroleum based polymer	214:258	the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	214:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	0	5	from	Effect	0:5	arg1	properties					74:83	the thermal, mechanical and biodegradation properties	31:83	the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite	31:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	1	6	from	abundant	311:318	arg1	nature					366:371	nature	366:371	nature	366:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	6	7	theme	grass	967:971	arg1	fibres					973:978	the cogon grass fibres	957:978	the cogon grass fibres	957:978	The thermal properties of the biocomposite were reduced as the cogon grass fibres increase from 0 to 5%.					
31730973	9	8	dep	biodegradable	1330:1342	arg1	composites					1226:1235	CGF/TPCS biopolymer composites	1206:1235	CGF/TPCS biopolymer composites	1206:1235	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	8	dep	biodegradable	1330:1342	arg1	material					1316:1323	environmental-friendly material	1293:1323	environmental-friendly material that biodegradable and renewable	1293:1356	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	9	theme	great	1274:1278	arg1	potential					1280:1288	great potential	1274:1288	great potential	1274:1288	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	6	10	theme	cogon	961:965	arg1	fibres					973:978	the cogon grass fibres	957:978	the cogon grass fibres	957:978	The thermal properties of the biocomposite were reduced as the cogon grass fibres increase from 0 to 5%.					
31730973	0	11	theme	cassava	102:108	arg1	biocomposite					117:128	thermoplastic cassava starch biocomposite	88:128	thermoplastic cassava starch biocomposite	88:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	7	12	theme	fibre	1044:1048	arg1	adhesion					1050:1057	good fibre adhesion	1039:1057	good fibre adhesion between CGF and TPCS	1039:1078	In term of morphological, SEM shows good fibre adhesion between CGF and TPCS.					
31730973	0	13	theme	thermoplastic	88:100	arg1	biocomposite					117:128	thermoplastic cassava starch biocomposite	88:128	thermoplastic cassava starch biocomposite	88:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	1	14	dep	abundant	311:318	arg1	i.e.					306:309	i.e.	306:309	i.e.	306:309	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	4	15	theme	CFG/TPCS	592:599	arg1	composites					612:621	CFG/TPCS biopolymer composites	592:621	CFG/TPCS biopolymer composites	592:621	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	5	16	theme	tensile	787:793	arg1	properties					808:817	the tensile and flexural properties	783:817	the tensile and flexural properties of the TPCS composites	783:840	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	8	17	theme	Soil	1081:1084	arg1	test					1093:1096	Soil burial test	1081:1096	Soil burial test	1081:1096	Soil burial test shows that incorporation of CGF into TPCS has slow down the biodegradation process of the composites.					
31730973	8	18	theme	composites	1188:1197	arg1	process					1173:1179	the biodegradation process	1154:1179	the biodegradation process of the composites	1154:1197	Soil burial test shows that incorporation of CGF into TPCS has slow down the biodegradation process of the composites.					
31730973	8	19	theme	CGF	1126:1128	arg1	incorporation					1109:1121	incorporation	1109:1121	incorporation of CGF into TPCS	1109:1138	Soil burial test shows that incorporation of CGF into TPCS has slow down the biodegradation process of the composites.					
31730973	1	20	theme	good	271:274	arg1	aspect					299:304	its good environmental-friendly aspect	267:304	its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	267:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	7	21	theme	good	1039:1042	arg1	adhesion					1050:1057	good fibre adhesion	1039:1057	good fibre adhesion between CGF and TPCS	1039:1078	In term of morphological, SEM shows good fibre adhesion between CGF and TPCS.					
31730973	1	22	theme	promising	172:180	arg1	material					194:201	a promising alternative material	170:201	a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	170:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	22	theme	promising	172:180	arg1	starch					153:158	Thermoplastic cassava starch	131:158	Thermoplastic cassava starch (TPCS)	131:165	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	23	from	nature	366:371	arg1	abundant					311:318	abundant	311:318	abundant	311:318	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	24	theme	environmental-friendly	276:297	arg1	aspect					299:304	its good environmental-friendly aspect	267:304	its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	267:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	0	25	theme	biocomposite	117:128	arg1	properties					74:83	the thermal, mechanical and biodegradation properties	31:83	the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite	31:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	3	26	theme	compression	536:546	arg1	molding					548:554	compression molding	536:554	compression molding	536:554	Therefore, in the present study, cogon grass fibre (CGF) were incorporated into TPCS using compression molding.					
31730973	1	27	theme	alternative	182:192	arg1	material					194:201	a promising alternative material	170:201	a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	170:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	27	theme	alternative	182:192	arg1	starch					153:158	Thermoplastic cassava starch	131:158	Thermoplastic cassava starch (TPCS)	131:165	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	3	28	theme	present	463:469	arg1	study					471:475	the present study	459:475	the present study	459:475	Therefore, in the present study, cogon grass fibre (CGF) were incorporated into TPCS using compression molding.					
31730973	0	29	theme	starch	110:115	arg1	biocomposite					117:128	thermoplastic cassava starch biocomposite	88:128	thermoplastic cassava starch biocomposite	88:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	2	30	theme	poor	417:420	arg1	properties					433:442	poor mechanical properties	417:442	poor mechanical properties	417:442	However, TPCS have some limitation such as poor mechanical properties.					
31730973	5	31	theme	CFG	766:768	arg1	incorporation					749:761	the incorporation	745:761	the incorporation of CFG	745:768	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	9	32	theme	environmental-friendly	1293:1314	arg1	composites					1226:1235	CGF/TPCS biopolymer composites	1206:1235	CGF/TPCS biopolymer composites	1206:1235	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	32	theme	environmental-friendly	1293:1314	arg1	material					1316:1323	environmental-friendly material	1293:1323	environmental-friendly material that biodegradable and renewable	1293:1356	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	2	33	contain	have	388:391	arg1	TPCS					383:386	TPCS	383:386	TPCS	383:386	However, TPCS have some limitation such as poor mechanical properties.					
31730973	2	33	contain	have	388:391	arg2	limitation					398:407	some limitation	393:407	some limitation	393:407	However, TPCS have some limitation such as poor mechanical properties.					
31730973	5	34	theme	impact	853:858	arg1	strength					860:867	the impact strength	849:867	the impact strength	849:867	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	5	35	theme	TPCS	826:829	arg1	composites					831:840	the TPCS composites	822:840	the TPCS composites	822:840	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	0	36	theme	fibre	22:26	arg1	Effect					0:5	Effect	0:5	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.	0:129	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	6	37	theme	biocomposite	928:939	arg1	properties					910:919	The thermal properties	898:919	The thermal properties of the biocomposite	898:939	The thermal properties of the biocomposite were reduced as the cogon grass fibres increase from 0 to 5%.					
31730973	7	38	theme	morphological	1014:1026	arg1	term					1006:1009	term	1006:1009	term of morphological	1006:1026	In term of morphological, SEM shows good fibre adhesion between CGF and TPCS.					
31730973	5	39	theme	flexural	799:806	arg1	properties					808:817	the tensile and flexural properties	783:817	the tensile and flexural properties of the TPCS composites	783:840	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	5	40	theme	composites	831:840	arg1	properties					808:817	the tensile and flexural properties	783:817	the tensile and flexural properties of the TPCS composites	783:840	From the study it was found that, the incorporation of CFG has improved the tensile and flexural properties of the TPCS composites, while the impact strength and elongation were reduced.					
31730973	0	41	theme	thermal	35:41	arg1	properties					74:83	the thermal, mechanical and biodegradation properties	31:83	the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite	31:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	6	42	dep	5	999:999	arg1	to					996:997	to	996:997	to	996:997	The thermal properties of the biocomposite were reduced as the cogon grass fibres increase from 0 to 5%.					
31730973	9	43	theme	biopolymer	1215:1224	arg1	composites					1258:1267	composites	1258:1267	composites with great potential	1258:1288	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	43	theme	biopolymer	1215:1224	arg1	composites					1226:1235	CGF/TPCS biopolymer composites	1206:1235	CGF/TPCS biopolymer composites	1206:1235	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	43	theme	biopolymer	1215:1224	arg1	material					1316:1323	environmental-friendly material	1293:1323	environmental-friendly material that biodegradable and renewable	1293:1356	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	4	44	dep	carried	628:634	arg1	out					636:638	out	636:638	out	636:638	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	9	45	with	composites	1258:1267	arg1	potential					1280:1288	great potential	1274:1288	great potential	1274:1288	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	4	46	theme	biopolymer	601:610	arg1	composites					612:621	CFG/TPCS biopolymer composites	592:621	CFG/TPCS biopolymer composites	592:621	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	1	47	theme	Thermoplastic	131:143	arg1	material					194:201	a promising alternative material	170:201	a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	170:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	47	theme	Thermoplastic	131:143	arg1	starch					153:158	Thermoplastic cassava starch	131:158	Thermoplastic cassava starch (TPCS)	131:165	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	47	theme	Thermoplastic	131:143	arg1	TPCS					161:164	TPCS	161:164	TPCS	161:164	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	48	theme	non-biodegradable	218:234	arg1	polymer					252:258	the non-biodegradable petroleum based polymer	214:258	the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	214:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	0	49	theme	mechanical	44:53	arg1	properties					74:83	the thermal, mechanical and biodegradation properties	31:83	the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite	31:128	Effect of cogon grass fibre on the thermal, mechanical and biodegradation properties of thermoplastic cassava starch biocomposite.					
31730973	9	50	theme	CGF/TPCS	1206:1213	arg1	composites					1258:1267	composites	1258:1267	composites with great potential	1258:1288	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	50	theme	CGF/TPCS	1206:1213	arg1	composites					1226:1235	CGF/TPCS biopolymer composites	1206:1235	CGF/TPCS biopolymer composites	1206:1235	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	9	50	theme	CGF/TPCS	1206:1213	arg1	material					1316:1323	environmental-friendly material	1293:1323	environmental-friendly material that biodegradable and renewable	1293:1356	Thus, CGF/TPCS biopolymer composites can be classified as composites with great potential as environmental-friendly material that biodegradable and renewable.					
31730973	2	51	theme	mechanical	422:431	arg1	properties					433:442	poor mechanical properties	417:442	poor mechanical properties	417:442	However, TPCS have some limitation such as poor mechanical properties.					
31730973	1	52	theme	cassava	145:151	arg1	material					194:201	a promising alternative material	170:201	a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	170:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	52	theme	cassava	145:151	arg1	starch					153:158	Thermoplastic cassava starch	131:158	Thermoplastic cassava starch (TPCS)	131:165	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	52	theme	cassava	145:151	arg1	TPCS					161:164	TPCS	161:164	TPCS	161:164	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	1	53	theme	petroleum	236:244	arg1	polymer					252:258	the non-biodegradable petroleum based polymer	214:258	the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	214:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31730973	8	54	theme	biodegradation	1158:1171	arg1	process					1173:1179	the biodegradation process	1154:1179	the biodegradation process of the composites	1154:1197	Soil burial test shows that incorporation of CGF into TPCS has slow down the biodegradation process of the composites.					
31730973	6	55	theme	thermal	902:908	arg1	properties					910:919	The thermal properties	898:919	The thermal properties of the biocomposite	898:939	The thermal properties of the biocomposite were reduced as the cogon grass fibres increase from 0 to 5%.					
31730973	4	56	theme	composites	612:621	arg1	properties					578:587	the fundamental properties	562:587	the fundamental properties of CFG/TPCS biopolymer composites	562:621	Then the fundamental properties of CFG/TPCS biopolymer composites were carried out in order to evaluate their potential as a biodegradable reinforcement.					
31730973	1	57	theme	based	246:250	arg1	polymer					252:258	the non-biodegradable petroleum based polymer	214:258	the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature	214:371	Thermoplastic cassava starch (TPCS) is a promising alternative material to replace the non-biodegradable petroleum based polymer due to its good environmental-friendly aspect i.e. abundant, sustainable, recyclable and biodegradable in nature.					
31760014	3	0	theme	-α-Araf-	688:695	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	0	theme	-α-Araf-	688:695	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	1	1	theme	novel	161:165	arg1	polysaccharide					179:192	a novel homogeneous polysaccharide	159:192	a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa)	159:217	In this study, a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa) was isolated and purified from the seeds of Litchi chinensis Sonn.					
31760014	0	2	from	Isolation	0:8	arg1	seeds					112:116	the seeds	108:116	the seeds of Litchi chinensis Sonn	108:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	2	3	theme	Monosaccharide	286:299	arg1	analysis					313:320	Monosaccharide composition analysis	286:320	Monosaccharide composition analysis	286:320	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	4	4	theme	mammalian	861:869	arg1	α-glucosidase					903:915	yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase	824:915	yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase	824:915	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	5	5	theme	KM	1206:1207	arg1	values					1209:1214	KM values	1206:1214	KM values	1206:1214	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	1	6	theme	homogeneous	167:177	arg1	polysaccharide					179:192	a novel homogeneous polysaccharide	159:192	a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa)	159:217	In this study, a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa) was isolated and purified from the seeds of Litchi chinensis Sonn.					
31760014	3	7	theme	structural	480:489	arg1	analysis					491:498	A detailed structural analysis	469:498	A detailed structural analysis	469:498	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	8	contain	has	522:524	arg1	LSP-W-4					514:520	LSP-W-4	514:520	LSP-W-4	514:520	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	8	contain	has	522:524	arg2	backbone					528:535	a backbone	526:535	a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp	526:575	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	8	contain	has	522:524	arg2	chains					603:608	four branched chains	589:608	four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	589:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	4	9	theme	yeast	824:828	arg1	α-glucosidase					903:915	yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase	824:915	yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase	824:915	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	5	10	theme	non-competitive	1177:1191	arg1	manner					1193:1198	a typical non-competitive manner	1167:1198	a typical non-competitive manner	1167:1198	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	5	11	theme	α-glucosidase	1150:1162	arg1	activities					1111:1120	the activities	1107:1120	the activities of both yeast and mammalian α-glucosidase	1107:1162	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	3	12	theme	1 → 5	682:686	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	12	theme	1 → 5	682:686	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	2	13	theme	6.33:3.88:10.35:1.00	447:466	arg1	ratio					438:442	a molar ratio	430:442	a molar ratio of 6.33:3.88:10.35:1.00	430:466	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	4	14	theme	inhibitory	990:999	arg1	activities					1001:1010	both such inhibitory activities	980:1010	both such inhibitory activities	980:1010	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	1	15	theme	Sonn	280:283	arg1	seeds					254:258	the seeds	250:258	the seeds of Litchi chinensis Sonn	250:283	In this study, a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa) was isolated and purified from the seeds of Litchi chinensis Sonn.					
31760014	4	16	theme	significant	779:789	arg1	activity					802:809	significant inhibitory activity	779:809	significant inhibitory activity	779:809	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	2	17	theme	molar	432:436	arg1	ratio					438:442	a molar ratio	430:442	a molar ratio of 6.33:3.88:10.35:1.00	430:466	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	0	18	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	5	19	theme	typical	1169:1175	arg1	manner					1193:1198	a typical non-competitive manner	1167:1198	a typical non-competitive manner	1167:1198	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	0	20	theme	Sonn	138:141	arg1	seeds					112:116	the seeds	108:116	the seeds of Litchi chinensis Sonn	108:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	3	21	theme	1 → 5	651:655	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	21	theme	1 → 5	651:655	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	2	22	theme	composition	301:311	arg1	analysis					313:320	Monosaccharide composition analysis	286:320	Monosaccharide composition analysis	286:320	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	4	23	dep	α-glucosidase	903:915	arg1	both					811:814	both	811:814	both	811:814	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	3	24	theme	1 → attached	712:723	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	24	theme	1 → attached	712:723	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	4	25	theme	IC50	923:926	arg1	values					928:933	IC50 values	923:933	IC50 values	923:933	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	4	26	dep	mammalian	861:869	arg1	powder					895:900	rat-intestinal acetone powder	872:900	rat-intestinal acetone powder	872:900	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	4	27	theme	such	985:988	arg1	activities					1001:1010	both such inhibitory activities	980:1010	both such inhibitory activities	980:1010	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	3	28	dep	-α-Araf-	703:710	arg1	1 → 5					697:701	1 → 5	697:701	1 → 5	697:701	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	29	theme	α-Araf-	643:649	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	29	theme	α-Araf-	643:649	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	4	30	theme	inhibitory	791:800	arg1	activity					802:809	significant inhibitory activity	779:809	significant inhibitory activity	779:809	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	3	31	theme	detailed	471:478	arg1	analysis					491:498	A detailed structural analysis	469:498	A detailed structural analysis	469:498	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	0	32	theme	α-glucosidase	63:75	arg1	polysaccharide					88:101	a non-competitive α-glucosidase inhibitory polysaccharide	45:101	a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn	45:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	3	33	theme	1,4-α-Glcp	749:758	arg1	O6					728:729	O6	728:729	O6 of 1,4-β-Manp and 1,4-α-Glcp	728:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	4	34	theme	acetone	887:893	arg1	powder					895:900	rat-intestinal acetone powder	872:900	rat-intestinal acetone powder	872:900	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	4	35	theme	66.97 μM	951:958	arg1	values					928:933	IC50 values	923:933	IC50 values	923:933	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	3	36	theme	branched	594:601	arg1	chains					603:608	four branched chains	589:608	four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	589:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	2	37	from	mannose	396:402	arg1	ratio					438:442	a molar ratio	430:442	a molar ratio of 6.33:3.88:10.35:1.00	430:466	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	0	38	theme	non-competitive	47:61	arg1	polysaccharide					88:101	a non-competitive α-glucosidase inhibitory polysaccharide	45:101	a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn	45:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	5	39	theme	mammalian	1140:1148	arg1	α-glucosidase					1150:1162	mammalian α-glucosidase	1140:1162	mammalian α-glucosidase	1140:1162	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	4	40	theme	rat-intestinal	872:885	arg1	powder					895:900	rat-intestinal acetone powder	872:900	rat-intestinal acetone powder	872:900	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	3	41	theme	1 → and	666:672	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	41	theme	1 → and	666:672	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	1	42	dep	polysaccharide	179:192	arg1	MW = 6.70 kDa					204:216	MW = 6.70 kDa	204:216	MW = 6.70 kDa	204:216	In this study, a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa) was isolated and purified from the seeds of Litchi chinensis Sonn.					
31760014	1	42	dep	polysaccharide	179:192	arg1	LSP-W-4					195:201	LSP-W-4	195:201	LSP-W-4	195:201	In this study, a novel homogeneous polysaccharide (LSP-W-4, MW = 6.70 kDa) was isolated and purified from the seeds of Litchi chinensis Sonn.					
31760014	5	43	theme	yeast	1130:1134	arg1	activities					1111:1120	the activities	1107:1120	the activities of both yeast and mammalian α-glucosidase	1107:1162	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	5	44	theme	kinetic	1058:1064	arg1	analysis					1066:1073	A kinetic analysis	1056:1073	A kinetic analysis	1056:1073	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	3	45	theme	1,4-β-Manp	734:743	arg1	O6					728:729	O6	728:729	O6 of 1,4-β-Manp and 1,4-α-Glcp	728:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	46	theme	α-Araf-	674:680	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	46	theme	α-Araf-	674:680	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	2	47	from	glucose	405:411	arg1	ratio					438:442	a molar ratio	430:442	a molar ratio of 6.33:3.88:10.35:1.00	430:466	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	3	48	theme	-α-Araf-	657:664	arg1	T-α-Galp					623:630	T-α-Galp	623:630	T-α-Galp	623:630	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	48	theme	-α-Araf-	657:664	arg1	-α-Araf-					703:710	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	643:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	0	49	theme	polysaccharide	88:101	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	0	49	theme	polysaccharide	88:101	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	0	50	from	seeds	112:116	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	0	50	from	seeds	112:116	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	0	50	from	seeds	112:116	arg1	polysaccharide					88:101	a non-competitive α-glucosidase inhibitory polysaccharide	45:101	a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn	45:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	3	51	theme	T-α-Galp	623:630	arg1	backbone					528:535	a backbone	526:535	a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp	526:575	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	3	51	theme	T-α-Galp	623:630	arg1	chains					603:608	four branched chains	589:608	four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp	589:758	A detailed structural analysis revealed that LSP-W-4 has a backbone consisting of 1,4-α-Glcp and 1,4-β-Manp, as well as four branched chains including of T-α-Galp, T-α-Araf, α-Araf-(1 → 5)-α-Araf-(1 → and α-Araf-(1 → 5)-α-Araf-(1 → 5)-α-Araf-(1 → attached to O6 of 1,4-β-Manp and 1,4-α-Glcp.					
31760014	2	52	from	galactose	417:425	arg1	ratio					438:442	a molar ratio	430:442	a molar ratio of 6.33:3.88:10.35:1.00	430:466	Monosaccharide composition analysis indicated that LSP-W-4 is a heteropolysaccharide consisting of arabinose, mannose, glucose and galactose in a molar ratio of 6.33:3.88:10.35:1.00.					
31760014	5	53	theme	0.43 mmol/L	1219:1229	arg1	values					1209:1214	KM values	1206:1214	KM values	1206:1214	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760014	0	54	theme	inhibitory	77:86	arg1	polysaccharide					88:101	a non-competitive α-glucosidase inhibitory polysaccharide	45:101	a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn	45:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	0	55	from	characterization	25:40	arg1	seeds					112:116	the seeds	108:116	the seeds of Litchi chinensis Sonn	108:141	Isolation and structural characterization of a non-competitive α-glucosidase inhibitory polysaccharide from the seeds of Litchi chinensis Sonn.					
31760014	4	56	dep	yeast	824:828	arg1	cerevisiae					845:854	Saccharomyces cerevisiae	831:854	yeast (Saccharomyces cerevisiae)	824:855	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	4	57	theme	75.24 μM	938:945	arg1	values					928:933	IC50 values	923:933	IC50 values	923:933	LSP-W-4 exhibited significant inhibitory activity both against yeast (Saccharomyces cerevisiae) and mammalian (rat-intestinal acetone powder) α-glucosidase, with IC50 values of 75.24 μM and 66.97 μM, respectively, with both such inhibitory activities being more powerful than those of acarbose.					
31760014	5	58	theme	0.53 mmol/L	1235:1245	arg1	values					1209:1214	KM values	1206:1214	KM values	1206:1214	A kinetic analysis revealed that LSP-W-4 inhibited the activities of both yeast and mammalian α-glucosidase in a typical non-competitive manner, with KM values of 0.43 mmol/L and 0.53 mmol/L, respectively.					
31760029	7	0	with	agent	1316:1320	arg1	potential					1339:1347	synergistic potential	1327:1347	synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1327:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	1	1	theme	antioxidant	261:271	arg1	capacity					273:280	antioxidant capacity	261:280	antioxidant capacity	261:280	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	6	2	theme	DFM2	880:883	arg1	concentration					863:875	minimum bactericidal concentration	842:875	minimum bactericidal concentration of DFM2	842:883	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	2	theme	DFM2	880:883	arg1	MIC					833:835	MIC	833:835	MIC	833:835	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	2	theme	DFM2	880:883	arg1	concentration					818:830	The minimum inhibitory concentration	795:830	The minimum inhibitory concentration (MIC)	795:836	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	2	theme	DFM2	880:883	arg1	lower					890:894	lower	890:894	lower	890:894	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	3	3	theme	second	538:543	arg1	DFM2					553:556	DFM2	553:556	DFM2	553:556	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	3	3	theme	second	538:543	arg1	method					545:550	the second method	534:550	the second method (DFM2)	534:557	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	7	4	from	doses	1408:1412	arg1	animals					1448:1454	animals	1448:1454	animals	1448:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	4	from	doses	1408:1412	arg1	humans					1437:1442	humans	1437:1442	humans	1437:1442	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	5	5	theme	ions	705:708	arg1	activity					720:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	5	5	theme	ions	705:708	arg1	%					738:738	78.3%	734:738	78.3% for DFM1	734:747	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	6	6	from	chloramphenicol	1017:1031	arg1	strains					1054:1060	the gram negative strains	1036:1060	the gram negative strains	1036:1060	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	5	7	theme	chelating	710:718	arg1	activity					720:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	5	7	theme	chelating	710:718	arg1	%					738:738	78.3%	734:738	78.3% for DFM1	734:747	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	1	8	theme	antimicrobial	286:298	arg1	activity					300:307	antimicrobial activity	286:307	antimicrobial activity	286:307	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	4	9	theme	more	680:683	arg1	times					674:678	1.93 times	669:678	1.93 times more than DFM2	669:693	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	4	9	theme	more	680:683	arg1	value					614:618	The IC50 value	605:618	The IC50 value of DPPH radical scavenging activity for DFM1	605:663	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	5	10	from	concentration	780:792	arg1	%					757:757	89.4%	753:757	89.4% for DFM2	753:766	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	5	10	from	concentration	780:792	arg1	%					738:738	78.3%	734:738	78.3% for DFM1	734:747	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	5	10	from	concentration	780:792	arg1	activity					720:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	1	11	from	effect	153:158	arg1	composition					248:258	their chemical composition	233:258	their chemical composition	233:258	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	1	11	from	effect	153:158	arg1	capacity					273:280	antioxidant capacity	261:280	antioxidant capacity	261:280	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	1	11	from	effect	153:158	arg1	activity					300:307	antimicrobial activity	286:307	antimicrobial activity	286:307	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	1	11	from	effect	153:158	arg1	tenerrimum					219:228	Sargassum tenerrimum	209:228	Sargassum tenerrimum	209:228	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	1	12	theme	water-extraction	167:182	arg1	methods					184:190	two water-extraction methods	163:190	two water-extraction methods of fucoidan from Sargassum tenerrimum	163:228	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	7	13	with	combination	1352:1362	arg1	antibiotics					1369:1379	antibiotics	1369:1379	antibiotics	1369:1379	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	6	14	theme	minimum	842:848	arg1	concentration					863:875	minimum bactericidal concentration	842:875	minimum bactericidal concentration of DFM2	842:883	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	7	15	theme	prescribed	1397:1406	arg1	doses					1408:1412	the prescribed doses	1393:1412	the prescribed doses of antibiotic drugs in humans and animals	1393:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	6	16	theme	negative	1045:1052	arg1	strains					1054:1060	the gram negative strains	1036:1060	the gram negative strains	1036:1060	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	1	17	theme	methods	184:190	arg1	effect					153:158	the effect	149:158	the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity	149:307	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	7	18	theme	drugs	1428:1432	arg1	doses					1408:1412	the prescribed doses	1393:1412	the prescribed doses of antibiotic drugs in humans and animals	1393:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	6	19	theme	gram	1040:1043	arg1	strains					1054:1060	the gram negative strains	1036:1060	the gram negative strains	1036:1060	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	20	theme	bactericidal	850:861	arg1	concentration					863:875	minimum bactericidal concentration	842:875	minimum bactericidal concentration of DFM2	842:883	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	7	21	from	humans	1437:1442	arg1	doses					1408:1412	the prescribed doses	1393:1412	the prescribed doses of antibiotic drugs in humans and animals	1393:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	22	theme	synergistic	1104:1114	arg1	effect					1116:1121	a synergistic effect	1102:1121	a synergistic effect	1102:1121	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	6	23	dep	faecalis	930:937	arg1	DFM2					964:967	The DFM2	960:967	The DFM2	960:967	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	23	dep	faecalis	930:937	arg1	effective					978:986	effective	978:986	effective	978:986	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	7	24	theme	depolymerized	1237:1249	arg1	agent					1316:1320	effective antibacterial agent	1292:1320	effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1292:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	24	theme	depolymerized	1237:1249	arg1	fucoidans					1251:1259	depolymerized fucoidans	1237:1259	depolymerized fucoidans	1237:1259	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	1	25	theme	fucoidan	195:202	arg1	methods					184:190	two water-extraction methods	163:190	two water-extraction methods of fucoidan from Sargassum tenerrimum	163:228	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	5	26	theme	10 mg/mL	771:778	arg1	concentration					780:792	10 mg/mL concentration	771:792	10 mg/mL concentration	771:792	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	7	27	dep	growth	1174:1179	arg1	considered					1278:1287	considered	1278:1287	can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1261:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	3	28	dep	respectively	580:591	arg1	P < 0.05					594:601	P < 0.05	594:601	P < 0.05	594:601	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	7	29	with	combination	1126:1136	arg1	chloramphenicol					1143:1157	chloramphenicol	1143:1157	chloramphenicol	1143:1157	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	30	from	potential	1339:1347	arg1	combination					1352:1362	combination	1352:1362	combination with antibiotics	1352:1379	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	3	31	theme	first	510:514	arg1	DFM1					524:527	DFM1	524:527	DFM1	524:527	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	3	31	theme	first	510:514	arg1	method					516:521	the first method	506:521	the first method (DFM1)	506:528	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	0	32	theme	antioxidant	9:19	arg1	Study					0:4	Study	0:4	Study of antioxidant and antibacterial activities of depolymerized fucoidans	0:75	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	1	33	theme	hydrothermal	315:326	arg1	depolymerization					328:343	hydrothermal depolymerization	315:343	hydrothermal depolymerization	315:343	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	2	34	theme	concentration	389:401	arg1	number					350:355	The number	346:355	The number of extraction steps and ethanol concentration used for extraction	346:421	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	2	34	theme	concentration	389:401	arg1	different					428:436	different	428:436	different	428:436	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	0	35	theme	activities	39:48	arg1	Study					0:4	Study	0:4	Study of antioxidant and antibacterial activities of depolymerized fucoidans	0:75	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	6	36	theme	MIC	1004:1006	arg1	value					1008:1012	the MIC value	1000:1012	the MIC value of chloramphenicol on the gram negative strains than DFM1	1000:1070	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	37	theme	chloramphenicol	1017:1031	arg1	value					1008:1012	the MIC value	1000:1012	the MIC value of chloramphenicol on the gram negative strains than DFM1	1000:1070	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	4	38	theme	scavenging	636:645	arg1	activity					647:654	DPPH radical scavenging activity	623:654	DPPH radical scavenging activity	623:654	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	3	39	theme	fucoidan	483:490	arg1	yield					474:478	Extraction yield	463:478	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2)	463:557	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	0	40	theme	antibacterial	25:37	arg1	activities					39:48	antibacterial activities	25:48	antibacterial activities of depolymerized fucoidans	25:75	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	1	41	from	tenerrimum	219:228	arg1	effect					153:158	the effect	149:158	the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity	149:307	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	1	41	from	tenerrimum	219:228	arg1	methods					184:190	two water-extraction methods	163:190	two water-extraction methods of fucoidan from Sargassum tenerrimum	163:228	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	4	42	theme	radical	628:634	arg1	activity					647:654	DPPH radical scavenging activity	623:654	DPPH radical scavenging activity	623:654	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	0	43	theme	depolymerized	53:65	arg1	fucoidans					67:75	depolymerized fucoidans	53:75	depolymerized fucoidans	53:75	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	7	44	theme	coli	1196:1199	arg1	growth					1174:1179	the growth	1170:1179	the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1170:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	45	theme	synergistic	1327:1337	arg1	potential					1339:1347	synergistic potential	1327:1347	synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1327:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	46	theme	antibiotic	1417:1426	arg1	drugs					1428:1432	antibiotic drugs	1417:1432	antibiotic drugs in humans and animals	1417:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	2	47	theme	ethanol	381:387	arg1	concentration					389:401	ethanol concentration	381:401	ethanol concentration used for extraction	381:421	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	5	48	theme	Fe+2	700:703	arg1	ions					705:708	Fe+2 ions	700:708	The Fe+2 ions chelating activity	696:727	The Fe+2 ions chelating activity were 78.3% for DFM1 and 89.4% for DFM2 at 10 mg/mL concentration.					
31760029	6	49	theme	inhibitory	807:816	arg1	MIC					833:835	MIC	833:835	MIC	833:835	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	49	theme	inhibitory	807:816	arg1	concentration					818:830	The minimum inhibitory concentration	795:830	The minimum inhibitory concentration (MIC)	795:836	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	49	theme	inhibitory	807:816	arg1	lower					890:894	lower	890:894	lower	890:894	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	7	50	theme	study	1230:1234	arg1	results					1214:1220	the results	1210:1220	the results of this study	1210:1234	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	6	51	from	value	1008:1012	arg1	strains					1054:1060	the gram negative strains	1036:1060	the gram negative strains	1036:1060	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	52	theme	minimum	799:805	arg1	MIC					833:835	MIC	833:835	MIC	833:835	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	52	theme	minimum	799:805	arg1	concentration					818:830	The minimum inhibitory concentration	795:830	The minimum inhibitory concentration (MIC)	795:836	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	6	52	theme	minimum	799:805	arg1	lower					890:894	lower	890:894	lower	890:894	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	7	53	from	animals	1448:1454	arg1	doses					1408:1412	the prescribed doses	1393:1412	the prescribed doses of antibiotic drugs in humans and animals	1393:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	2	54	theme	steps	371:375	arg1	number					350:355	The number	346:355	The number of extraction steps and ethanol concentration used for extraction	346:421	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	2	54	theme	steps	371:375	arg1	different					428:436	different	428:436	different	428:436	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	6	55	from	strains	1054:1060	arg1	value					1008:1012	the MIC value	1000:1012	the MIC value of chloramphenicol on the gram negative strains than DFM1	1000:1070	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of DFM2 were lower compared to DFM1 for Enterococcus faecalis and Escherichia coli The DFM2 was more effective to decrease the MIC value of chloramphenicol on the gram negative strains than DFM1.					
31760029	2	56	theme	extraction	360:369	arg1	steps					371:375	extraction steps	360:375	extraction steps	360:375	The number of extraction steps and ethanol concentration used for extraction were different between the two methods.					
31760029	1	57	theme	chemical	239:246	arg1	composition					248:258	their chemical composition	233:258	their chemical composition	233:258	This work was performed to compare the effect of two water-extraction methods of fucoidan from Sargassum tenerrimum on their chemical composition, antioxidant capacity and antimicrobial activity after hydrothermal depolymerization.					
31760029	7	58	theme	effective	1292:1300	arg1	agent					1316:1320	effective antibacterial agent	1292:1320	effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1292:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	58	theme	effective	1292:1300	arg1	fucoidans					1251:1259	depolymerized fucoidans	1237:1259	depolymerized fucoidans	1237:1259	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	4	59	theme	DPPH	623:626	arg1	activity					647:654	DPPH radical scavenging activity	623:654	DPPH radical scavenging activity	623:654	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	4	60	theme	activity	647:654	arg1	times					674:678	1.93 times	669:678	1.93 times more than DFM2	669:693	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	4	60	theme	activity	647:654	arg1	value					614:618	The IC50 value	605:618	The IC50 value of DPPH radical scavenging activity for DFM1	605:663	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	0	61	theme	fucoidans	67:75	arg1	antioxidant					9:19	antioxidant	9:19	antioxidant	9:19	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	0	61	theme	fucoidans	67:75	arg1	activities					39:48	antibacterial activities	25:48	antibacterial activities of depolymerized fucoidans	25:75	Study of antioxidant and antibacterial activities of depolymerized fucoidans extracted from Sargassum tenerrimum.					
31760029	3	62	theme	Extraction	463:472	arg1	yield					474:478	Extraction yield	463:478	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2)	463:557	Extraction yield of fucoidan obtained from the first method (DFM1) and the second method (DFM2) were 3.68 and 1.09%, respectively (P < 0.05).					
31760029	7	63	theme	antibacterial	1302:1314	arg1	agent					1316:1320	effective antibacterial agent	1292:1320	effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals	1292:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	63	theme	antibacterial	1302:1314	arg1	fucoidans					1251:1259	depolymerized fucoidans	1237:1259	depolymerized fucoidans	1237:1259	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	64	from	drugs	1428:1432	arg1	animals					1448:1454	animals	1448:1454	animals	1448:1454	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	7	64	from	drugs	1428:1432	arg1	humans					1437:1442	humans	1437:1442	humans	1437:1442	Both of the fucoidans showed a synergistic effect in combination with chloramphenicol to inhibit the growth of Escherichia coli Based on the results of this study, depolymerized fucoidans can also be more considered as effective antibacterial agent with synergistic potential in combination with antibiotics to decrease the prescribed doses of antibiotic drugs in humans and animals.					
31760029	4	65	theme	IC50	609:612	arg1	times					674:678	1.93 times	669:678	1.93 times more than DFM2	669:693	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31760029	4	65	theme	IC50	609:612	arg1	value					614:618	The IC50 value	605:618	The IC50 value of DPPH radical scavenging activity for DFM1	605:663	The IC50 value of DPPH radical scavenging activity for DFM1 was 1.93 times more than DFM2.					
31454234	2	0	theme	crystals	663:670	arg1	addition					608:615	the addition	604:615	the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan	604:733	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	4	1	theme	%	1077:1077	arg1	strain					1079:1084	60% strain	1075:1084	work to 60% strain	1067:1084	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	2	2	theme	resulting	774:782	arg1	composites					784:793	the resulting composites	770:793	the resulting composites	770:793	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	3	link	plant-derived	620:632	arg1	CNF					657:659	CNF	657:659	CNF	657:659	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	3	link	plant-derived	620:632	arg1	fibrils					648:654	plant-derived nanocellulose fibrils	620:654	plant-derived nanocellulose fibrils (CNF)	620:660	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	1	4	theme	lightweight	230:240	arg1	structures					242:251	lightweight structures	230:251	lightweight structures	230:251	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	2	5	theme	nanocellulose	634:646	arg1	CNF					657:659	CNF	657:659	CNF	657:659	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	5	theme	nanocellulose	634:646	arg1	fibrils					648:654	plant-derived nanocellulose fibrils	620:654	plant-derived nanocellulose fibrils (CNF)	620:660	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	4	6	theme	compression	1106:1116	arg1	parallel					1118:1125	compression parallel	1106:1125	compression parallel	1106:1125	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	6	7	theme	nanocellulose	1365:1377	arg1	observed					1327:1334	observed	1327:1334	observed	1327:1334	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	6	7	theme	nanocellulose	1365:1377	arg1	self-assembly					1344:1356	the self-assembly	1340:1356	the self-assembly of the nanocellulose into microstruts and microbridges	1340:1411	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	3	8	theme	composite	819:827	arg1	scaffolds					829:837	Chitosan-nanocellulose composite scaffolds	796:837	Chitosan-nanocellulose composite scaffolds	796:837	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	1	9	from	insulation	308:317	arg1	range					289:293	range	289:293	range from thermal insulation to filtration	289:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	8	10	theme	nanocellulose	1802:1814	arg1	additions					1816:1824	the nanocellulose additions	1798:1824	the nanocellulose additions	1798:1824	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	1	11	theme	recent	155:160	arg1	interest					162:169	considerable recent interest	142:169	considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	142:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	7	12	theme	anisotropy	1638:1647	arg1	ratio					1649:1653	smallest anisotropy ratio	1629:1653	smallest anisotropy ratio	1629:1653	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	12	theme	anisotropy	1638:1647	arg1	modulus					1589:1595	the highest modulus	1577:1595	the highest modulus	1577:1595	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	3	13	dep	states	953:958	arg1	both					921:924	both	921:924	both	921:924	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	9	14	theme	latter	2016:2021	arg1	loads					2032:2036	The latter transfer loads	2012:2036	The latter transfer loads	2012:2036	The latter transfer loads and enable the scaffolds to resist deformation also perpendicular to the freezing direction.					
31454234	1	15	from	application	511:521	arg1	regeneration					533:544	tissue regeneration	526:544	tissue regeneration	526:544	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	1	15	from	application	511:521	arg1	general					492:498	general	492:498	general	492:498	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	8	16	theme	mechanical	1841:1850	arg1	properties					1852:1861	the mechanical properties	1837:1861	the mechanical properties of the scaffold through cell-wall material	1837:1904	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	10	17	theme	chitosan-nanocellulose	2229:2250	arg1	composites					2252:2261	chitosan-nanocellulose composites	2229:2261	chitosan-nanocellulose composites	2229:2261	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	6	18	theme	adjacent	1421:1428	arg1	lamellae					1442:1449	lamellae	1442:1449	lamellae	1442:1449	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	6	18	theme	adjacent	1421:1428	arg1	features					1453:1460	features	1453:1460	features that affected the mechanical properties of the scaffolds	1453:1517	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	6	18	theme	adjacent	1421:1428	arg1	walls					1435:1439	adjacent cell walls	1421:1439	adjacent cell walls (lamellae)	1421:1450	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	1	19	theme	structure-property-processing	387:415	arg1	correlations					417:428	structure-property-processing correlations	387:428	structure-property-processing correlations	387:428	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	5	20	theme	freezing	1293:1300	arg1	direction					1302:1310	the freezing direction	1289:1310	the freezing direction	1289:1310	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	0	21	theme	Biomedical	109:118	arg1	Applications					120:131	Biomedical Applications	109:131	Biomedical Applications	109:131	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	8	22	theme	scaffold	1870:1877	arg1	properties					1852:1861	the mechanical properties	1837:1861	the mechanical properties of the scaffold through cell-wall material	1837:1904	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	2	23	dep	structure	742:750	arg1	the					738:740	the	738:740	the	738:740	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	24	theme	blend	685:689	arg1	addition					608:615	the addition	604:615	the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan	604:733	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	1	25	theme	chitosan-nanocellulose	445:466	arg1	scaffolds					478:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	1	26	from	interest	162:169	arg1	cellulose					201:209	micro- and nanofibrillated cellulose	174:209	micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	174:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	3	27	theme	dry	930:932	arg1	states					953:958	both the dry and fully hydrated states	921:958	states	953:958	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	0	28	theme	Plant-Derived	0:12	arg1	Nanocellulose					14:26	Plant-Derived Nanocellulose	0:26	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan	0:93	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	3	29	theme	scanning	966:973	arg1	electron					975:982	scanning electron and confocal microscopy	966:1006	electron	975:982	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	1	30	theme	systematic	338:347	arg1	studies					349:355	few systematic studies	334:355	few systematic studies	334:355	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	4	31	theme	yield	1036:1040	arg1	strength					1042:1049	yield strength	1036:1049	yield strength	1036:1049	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	4	31	theme	yield	1036:1040	arg1	modulus					1027:1033	The modulus	1023:1033	The modulus	1023:1033	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	0	32	theme	Freeze-Cast	74:84	arg1	Chitosan					86:93	Freeze-Cast Chitosan	74:93	Freeze-Cast Chitosan	74:93	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	3	33	from	°C/min	868:873	arg1	freeze-cast					844:854	freeze-cast	844:854	freeze-cast	844:854	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	9	34	theme	freezing	2111:2118	arg1	direction					2120:2128	the freezing direction	2107:2128	the freezing direction	2107:2128	The latter transfer loads and enable the scaffolds to resist deformation also perpendicular to the freezing direction.					
31454234	10	35	theme	composites	2252:2261	arg1	promise					2218:2224	the promise	2214:2224	the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices	2214:2348	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	7	36	from	°C/min	1542:1547	arg1	freeze-cast					1525:1535	freeze-cast	1525:1535	freeze-cast	1525:1535	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	4	37	theme	60	1075:1076	arg1	%					1077:1077	%	1077:1077	%	1077:1077	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	1	38	theme	thermal	300:306	arg1	insulation					308:317	thermal insulation	300:317	thermal insulation to filtration	300:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	1	39	theme	tissue	526:531	arg1	regeneration					533:544	tissue regeneration	526:544	tissue regeneration	526:544	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	5	40	theme	preferential	1230:1241	arg1	alignments					1243:1252	the preferential alignments	1226:1252	the preferential alignments of CNCs and/or fibrils parallel to the freezing direction	1226:1310	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	5	40	theme	preferential	1230:1241	arg1	Observed					1212:1219	Observed	1212:1219	Observed	1212:1219	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	10	41	theme	biomedical	2318:2327	arg1	implants					2329:2336	biomedical implants	2318:2336	biomedical implants	2318:2336	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	2	42	theme	composites	784:793	arg1	structure					742:750	structure	742:750	structure	742:750	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	42	theme	composites	784:793	arg1	properties					756:765	properties	756:765	properties	756:765	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	10	43	theme	property	2144:2151	arg1	profile					2153:2159	The observed property profile	2131:2159	The observed property profile	2131:2159	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	4	44	dep	work	1067:1070	arg1	to					1072:1073	to	1072:1073	to	1072:1073	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	4	44	dep	work	1067:1070	arg1	strain					1079:1084	60% strain	1075:1084	work to 60% strain	1067:1084	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	4	45	dep	toughness	1056:1064	arg1	work					1067:1070	work	1067:1070	work to 60% strain	1067:1084	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	2	46	theme	two	698:700	arg1	blend					685:689	a blend	683:689	a blend of the two (CNB)	683:706	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	46	theme	two	698:700	arg1	CNCs					673:676	CNCs	673:676	CNCs	673:676	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	46	theme	two	698:700	arg1	CNF					657:659	CNF	657:659	CNF	657:659	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	46	theme	two	698:700	arg1	crystals					663:670	crystals	663:670	crystals (CNCs)	663:677	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	46	theme	two	698:700	arg1	fibrils					648:654	plant-derived nanocellulose fibrils	620:654	plant-derived nanocellulose fibrils (CNF)	620:660	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	1	47	theme	structures	242:251	arg1	constituents					214:225	constituents	214:225	constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	214:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	2	48	theme	plant-derived	620:632	arg1	CNF					657:659	CNF	657:659	CNF	657:659	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	48	theme	plant-derived	620:632	arg1	fibrils					648:654	plant-derived nanocellulose fibrils	620:654	plant-derived nanocellulose fibrils (CNF)	620:660	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	1	49	theme	scaffolds	257:265	arg1	constituents					214:225	constituents	214:225	constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	214:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	2	50	theme	addition	608:615	arg1	effects					593:599	the effects	589:599	the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites	589:793	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	1	51	theme	considerable	142:153	arg1	interest					162:169	considerable recent interest	142:169	considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	142:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	7	52	theme	smallest	1629:1636	arg1	ratio					1649:1653	smallest anisotropy ratio	1629:1653	smallest anisotropy ratio	1629:1653	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	52	theme	smallest	1629:1636	arg1	modulus					1589:1595	the highest modulus	1577:1595	the highest modulus	1577:1595	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	2	53	from	effects	593:599	arg1	structure					742:750	structure	742:750	structure	742:750	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	2	53	from	effects	593:599	arg1	properties					756:765	properties	756:765	properties	756:765	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	7	54	theme	chitosan-CNF	1550:1561	arg1	scaffolds					1563:1571	chitosan-CNF scaffolds	1550:1571	chitosan-CNF scaffolds	1550:1571	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	2	55	theme	biopolymer	715:724	arg1	chitosan					726:733	the biopolymer chitosan	711:733	the biopolymer chitosan	711:733	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	3	56	theme	Chitosan-nanocellulose	796:817	arg1	scaffolds					829:837	Chitosan-nanocellulose composite scaffolds	796:837	Chitosan-nanocellulose composite scaffolds	796:837	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	2	57	theme	fibrils	648:654	arg1	addition					608:615	the addition	604:615	the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan	604:733	Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites.					
31454234	4	58	theme	freezing	1169:1176	arg1	direction					1178:1186	the freezing direction	1165:1186	the freezing direction	1165:1186	The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy.					
31454234	10	59	theme	proven	2180:2185	arg1	biocompatibility					2187:2202	the materials' proven biocompatibility	2165:2202	the materials' proven biocompatibility	2165:2202	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	0	60	theme	Chitosan	86:93	arg1	Reinforcement					57:69	Structural and Mechanical Reinforcement	31:69	Structural and Mechanical Reinforcement of Freeze-Cast Chitosan	31:93	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	7	61	contain	had	1573:1575	arg2	modulus					1589:1595	the highest modulus	1577:1595	the highest modulus	1577:1595	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	61	contain	had	1573:1575	arg2	toughness					1614:1622	toughness	1614:1622	toughness	1614:1622	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	61	contain	had	1573:1575	arg2	strength					1604:1611	yield strength	1598:1611	yield strength	1598:1611	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	61	contain	had	1573:1575	arg1	scaffolds					1563:1571	chitosan-CNF scaffolds	1550:1571	chitosan-CNF scaffolds	1550:1571	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	61	contain	had	1573:1575	arg2	ratio					1649:1653	smallest anisotropy ratio	1629:1653	smallest anisotropy ratio	1629:1653	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	1	62	theme	micro-	174:179	arg1	cellulose					201:209	micro- and nanofibrillated cellulose	174:209	micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	174:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	9	63	theme	transfer	2023:2030	arg1	loads					2032:2036	The latter transfer loads	2012:2036	The latter transfer loads	2012:2036	The latter transfer loads and enable the scaffolds to resist deformation also perpendicular to the freezing direction.					
31454234	5	64	theme	parallel	1277:1284	arg1	CNCs					1257:1260	CNCs	1257:1260	CNCs	1257:1260	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	6	65	theme	cell	1430:1433	arg1	lamellae					1442:1449	lamellae	1442:1449	lamellae	1442:1449	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	6	65	theme	cell	1430:1433	arg1	features					1453:1460	features	1453:1460	features that affected the mechanical properties of the scaffolds	1453:1517	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	6	65	theme	cell	1430:1433	arg1	walls					1435:1439	adjacent cell walls	1421:1439	adjacent cell walls (lamellae)	1421:1450	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	1	66	theme	nanofibrillated	185:199	arg1	cellulose					201:209	micro- and nanofibrillated cellulose	174:209	micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration	174:331	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	7	67	theme	crystalline	1745:1755	arg1	cellulose					1757:1765	crystalline cellulose	1745:1765	crystalline cellulose	1745:1765	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	68	theme	yield	1598:1602	arg1	modulus					1589:1595	the highest modulus	1577:1595	the highest modulus	1577:1595	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	68	theme	yield	1598:1602	arg1	strength					1604:1611	yield strength	1598:1611	yield strength	1598:1611	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	8	69	theme	architectural	1946:1958	arg1	pillars					1989:1995	pillars	1989:1995	pillars	1989:1995	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	8	69	theme	architectural	1946:1958	arg1	features					1960:1967	architectural features	1946:1967	architectural features	1946:1967	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	8	69	theme	architectural	1946:1958	arg1	bridges					1977:1983	bridges	1977:1983	bridges	1977:1983	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	10	70	theme	applications	2284:2295	arg1	those					2308:2312	those	2308:2312	those	2308:2312	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	10	70	theme	applications	2284:2295	arg1	range					2275:2279	a large range	2267:2279	a large range of applications, including those for biomedical implants and devices	2267:2348	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	1	71	from	scaffolds	478:486	arg1	regeneration					533:544	tissue regeneration	526:544	tissue regeneration	526:544	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	1	71	from	scaffolds	478:486	arg1	general					492:498	general	492:498	general	492:498	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	1	72	theme	freeze-cast	433:443	arg1	scaffolds					478:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	3	73	theme	hydrated	944:951	arg1	states					953:958	both the dry and fully hydrated states	921:958	states	953:958	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	6	74	theme	mechanical	1480:1489	arg1	properties					1491:1500	the mechanical properties	1476:1500	the mechanical properties of the scaffolds	1476:1517	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	1	75	theme	composite	468:476	arg1	scaffolds					478:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	freeze-cast chitosan-nanocellulose composite scaffolds	433:486	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	7	76	theme	nanocellulose	1710:1722	arg1	blend					1724:1728	the nanocellulose blend	1706:1728	the nanocellulose blend	1706:1728	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	0	77	theme	Structural	31:40	arg1	Reinforcement					57:69	Structural and Mechanical Reinforcement	31:69	Structural and Mechanical Reinforcement of Freeze-Cast Chitosan	31:93	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	1	78	theme	few	334:336	arg1	studies					349:355	few systematic studies	334:355	few systematic studies	334:355	Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular.					
31454234	10	79	theme	large	2269:2273	arg1	those					2308:2312	those	2308:2312	those	2308:2312	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	10	79	theme	large	2269:2273	arg1	range					2275:2279	a large range	2267:2279	a large range of applications, including those for biomedical implants and devices	2267:2348	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	0	80	theme	Mechanical	46:55	arg1	Reinforcement					57:69	Structural and Mechanical Reinforcement	31:69	Structural and Mechanical Reinforcement of Freeze-Cast Chitosan	31:93	Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.					
31454234	7	81	theme	highest	1581:1587	arg1	ratio					1649:1653	smallest anisotropy ratio	1629:1653	smallest anisotropy ratio	1629:1653	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	81	theme	highest	1581:1587	arg1	toughness					1614:1622	toughness	1614:1622	toughness	1614:1622	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	81	theme	highest	1581:1587	arg1	modulus					1589:1595	the highest modulus	1577:1595	the highest modulus	1577:1595	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	7	81	theme	highest	1581:1587	arg1	strength					1604:1611	yield strength	1598:1611	yield strength	1598:1611	When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose.					
31454234	8	82	theme	cell-wall	1887:1895	arg1	material					1897:1904	cell-wall material	1887:1904	cell-wall material	1887:1904	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	3	83	theme	confocal	988:995	arg1	microscopy					997:1006	scanning electron and confocal microscopy	966:1006	microscopy	997:1006	Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively.					
31454234	5	84	theme	CNCs	1257:1260	arg1	alignments					1243:1252	the preferential alignments	1226:1252	the preferential alignments of CNCs and/or fibrils parallel to the freezing direction	1226:1310	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	5	84	theme	CNCs	1257:1260	arg1	Observed					1212:1219	Observed	1212:1219	Observed	1212:1219	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	10	85	theme	observed	2135:2142	arg1	profile					2153:2159	The observed property profile	2131:2159	The observed property profile	2131:2159	The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.					
31454234	8	86	from	features	1960:1967	arg1	other					2005:2009	other	2005:2009	other	2005:2009	These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other.					
31454234	6	87	theme	scaffolds	1509:1517	arg1	properties					1491:1500	the mechanical properties	1476:1500	the mechanical properties of the scaffolds	1476:1517	Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds.					
31454234	5	88	theme	fibrils	1269:1275	arg1	alignments					1243:1252	the preferential alignments	1226:1252	the preferential alignments of CNCs and/or fibrils parallel to the freezing direction	1226:1310	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
31454234	5	88	theme	fibrils	1269:1275	arg1	Observed					1212:1219	Observed	1212:1219	Observed	1212:1219	Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction.					
29773361	6	0	theme	staining	903:910	arg1	observation					912:922	H&E staining observation	899:922	H&E staining observation	899:922	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	2	1	from	degradation	392:402	arg1	microclimate					369:380	the acid microclimate	360:380	the acid microclimate from PLLA degradation	360:402	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	2	2	theme	chitosan	275:282	arg1	microspheres					284:295	chitosan microspheres	275:295	chitosan microspheres (CSMs)	275:302	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	2	2	theme	chitosan	275:282	arg1	CSMs					298:301	CSMs	298:301	CSMs	298:301	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	8	3	theme	degradation	1073:1083	arg1	behavior					1085:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	8	4	theme	moderate	1098:1105	arg1	microclimate					1119:1130	moderate degradation microclimate	1098:1130	moderate degradation microclimate	1098:1130	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	3	5	dep	in	418:419	arg1	vivo					421:424	vivo	421:424	vivo	421:424	In vitro and in vivo degradation were, respectively, performed in PBS and implanted into rat's subcutaneous.					
29773361	8	6	theme	tunable	1065:1071	arg1	behavior					1085:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	6	7	theme	in	806:807	arg1	degradation					794:804	degradation	794:804	CSMs/PLLA composites degradation in vivo	773:812	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	1	8	theme	popular	132:138	arg1	Poly					100:103	Poly	100:103	Poly(L-lactic acid) (PLLA)	100:125	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	8	theme	popular	132:138	arg1	polymer					154:160	a popular biodegradable polymer	130:160	a popular biodegradable polymer	130:160	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	7	9	theme	PLLA	1055:1058	arg1	degradation					1040:1050	the degradation	1036:1050	the degradation of PLLA	1036:1058	These results indicated that incorporation of CSMs not only buffered the acidic microclimate but also improved the degradation of PLLA.					
29773361	2	10	theme	PLLA	387:390	arg1	degradation					392:402	PLLA degradation	387:402	PLLA degradation	387:402	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	2	11	theme	porous	330:335	arg1	matrices					337:344	PLLA porous matrices	325:344	PLLA porous matrices	325:344	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	8	12	theme	degradation	1107:1117	arg1	microclimate					1119:1130	moderate degradation microclimate	1098:1130	moderate degradation microclimate	1098:1130	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	6	13	theme	CSMs/PLLA	773:781	arg1	composites					783:792	CSMs/PLLA composites	773:792	CSMs/PLLA composites degradation in vivo	773:812	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	5	14	dep	loss	659:662	arg1	mass					654:657	the increasing of CSMs, mass loss	630:662	mass	654:657	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	2	15	theme	PLLA	325:328	arg1	matrices					337:344	PLLA porous matrices	325:344	PLLA porous matrices	325:344	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	0	16	theme	Degradation	0:10	arg1	properties					12:21	Degradation properties	0:21	Degradation properties of chitosan	0:33	Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo.					
29773361	7	17	theme	acidic	998:1003	arg1	microclimate					1005:1016	the acidic microclimate	994:1016	the acidic microclimate	994:1016	These results indicated that incorporation of CSMs not only buffered the acidic microclimate but also improved the degradation of PLLA.					
29773361	1	18	theme	biodegradable	140:152	arg1	Poly					100:103	Poly	100:103	Poly(L-lactic acid) (PLLA)	100:125	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	18	theme	biodegradable	140:152	arg1	polymer					154:160	a popular biodegradable polymer	130:160	a popular biodegradable polymer	130:160	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	0	19	theme	chitosan	26:33	arg1	properties					12:21	Degradation properties	0:21	Degradation properties of chitosan	0:33	Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo.					
29773361	0	20	dep	composite	68:76	arg1	acid					62:65	L-lactic acid	53:65	L-lactic acid	53:65	Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo.					
29773361	2	21	theme	acid	364:367	arg1	microclimate					369:380	the acid microclimate	360:380	the acid microclimate from PLLA degradation	360:402	In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation.					
29773361	5	22	theme	PLLA	723:726	arg1	Mv					717:718	Mv	717:718	Mv of PLLA	717:726	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	1	23	theme	extended	238:245	arg1	utilization					247:257	its extended utilization	234:257	its extended utilization	234:257	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	4	24	theme	composites	557:566	arg1	pH					541:542	pH	541:542	pH of CSMs/PLLA composites	541:566	The results indicated that pH of CSMs/PLLA composites remained stable of around 7.50 in vitro until six weeks.					
29773361	8	25	theme	composites	1145:1154	arg1	microclimate					1119:1130	moderate degradation microclimate	1098:1130	moderate degradation microclimate	1098:1130	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	8	25	theme	composites	1145:1154	arg1	behavior					1085:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior	1061:1092	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	6	26	theme	CSMs	854:857	arg1	introduction					838:849	the introduction	834:849	the introduction of CSMs via obvious morphological destroy under H&E staining observation	834:922	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	5	27	theme	increasing	634:643	arg1	loss					659:662	the increasing of CSMs, mass loss	630:662	loss	659:662	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	8	28	theme	biomedical	1210:1219	arg1	application					1221:1231	its biomedical application	1206:1231	its biomedical application	1206:1231	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	5	29	theme	in	745:746	arg1	test					754:757	in vitro test	745:757	in vitro test	745:757	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	5	30	theme	composites	689:698	arg1	absorption					671:680	water absorption	665:680	water absorption of the composites	665:698	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	6	31	theme	H&E	899:901	arg1	staining					903:910	H&E staining	899:910	H&E staining observation	899:922	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	6	32	dep	in	806:807	arg1	vivo					809:812	vivo	809:812	vivo	809:812	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	3	33	theme	In	405:406	arg1	degradation					426:436	In vitro and in vivo degradation	405:436	In vitro and in vivo degradation	405:436	In vitro and in vivo degradation were, respectively, performed in PBS and implanted into rat's subcutaneous.					
29773361	8	34	theme	CSMs/PLLA	1135:1143	arg1	composites					1145:1154	CSMs/PLLA composites	1135:1154	CSMs/PLLA composites developed in the study	1135:1177	The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.					
29773361	0	35	theme	L-lactic	53:60	arg1	acid					62:65	L-lactic acid	53:65	L-lactic acid	53:65	Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo.					
29773361	3	36	theme	in	418:419	arg1	degradation					426:436	In vitro and in vivo degradation	405:436	In vitro and in vivo degradation	405:436	In vitro and in vivo degradation were, respectively, performed in PBS and implanted into rat's subcutaneous.					
29773361	5	37	dep	in	745:746	arg1	vitro					748:752	vitro	748:752	vitro	748:752	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	1	38	theme	L-lactic	105:112	arg1	Poly					100:103	Poly	100:103	Poly(L-lactic acid) (PLLA)	100:125	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	38	theme	L-lactic	105:112	arg1	acid					114:117	L-lactic acid	105:117	L-lactic acid	105:117	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	39	theme	accumulated	171:181	arg1	production					188:197	the accumulated acid production	167:197	the accumulated acid production by degradation	167:212	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	39	theme	accumulated	171:181	arg1	bottleneck					219:228	a bottleneck	217:228	a bottleneck for its extended utilization	217:257	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	6	40	dep	morphological	871:883	arg1	destroy					885:891	destroy	885:891	destroy under H&E staining observation	885:922	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
29773361	3	41	dep	In	405:406	arg1	vitro					408:412	vitro	408:412	vitro	408:412	In vitro and in vivo degradation were, respectively, performed in PBS and implanted into rat's subcutaneous.					
29773361	1	42	theme	acid	183:186	arg1	production					188:197	the accumulated acid production	167:197	the accumulated acid production by degradation	167:212	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	1	42	theme	acid	183:186	arg1	bottleneck					219:228	a bottleneck	217:228	a bottleneck for its extended utilization	217:257	Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization.					
29773361	4	43	theme	CSMs/PLLA	547:555	arg1	composites					557:566	CSMs/PLLA composites	547:566	CSMs/PLLA composites	547:566	The results indicated that pH of CSMs/PLLA composites remained stable of around 7.50 in vitro until six weeks.					
29773361	5	44	theme	water	665:669	arg1	absorption					671:680	water absorption	665:680	water absorption of the composites	665:698	With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test.					
29773361	7	45	theme	CSMs	971:974	arg1	incorporation					954:966	incorporation	954:966	incorporation of CSMs	954:974	These results indicated that incorporation of CSMs not only buffered the acidic microclimate but also improved the degradation of PLLA.					
29773361	6	46	dep	composites	783:792	arg1	degradation					794:804	degradation	794:804	CSMs/PLLA composites degradation in vivo	773:812	Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation.					
31591306	3	0	from	changes	763:769	arg1	capacity					786:793	antioxidant capacity	774:793	antioxidant capacity	774:793	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	0	from	changes	763:769	arg1	production					805:814	SCFAs production	799:814	SCFAs production	799:814	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	0	1	from	Fermentation	9:20	arg1	vera					51:54	Aloe vera	46:54	Aloe vera	46:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	5	2	dep	in	1077:1078	arg1	vitro					1080:1084	vitro	1080:1084	vitro	1080:1084	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	2	3	theme	fatty	475:479	arg1	SCFAs					488:492	SCFAs	488:492	SCFAs	488:492	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	2	3	theme	fatty	475:479	arg1	acids					481:485	short chain fatty acids	463:485	short chain fatty acids (SCFAs)	463:493	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	2	4	theme	Bifidobacterium	329:343	arg1	strains					363:369	Bifidobacterium and Lactobacillus strains	329:369	strains	363:369	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	5	5	theme	total	1172:1176	arg1	volume					1178:1183	the total volume	1168:1183	the total volume of gas produced	1168:1199	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	6	theme	Activity	90:97	arg1	Fermentation					9:20	In vitro Fermentation	0:20	In vitro Fermentation of Polysaccharides from Aloe vera	0:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	6	theme	Activity	90:97	arg1	Evaluation					64:73	the Evaluation	60:73	the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids	60:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	5	7	theme	AV	1131:1132	arg1	fibre					1122:1126	fibre	1122:1126	fibre	1122:1126	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	8	theme	Antioxidant	78:88	arg1	Activity					90:97	Antioxidant Activity	78:97	Antioxidant Activity	78:97	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	9	from	vera	51:54	arg1	Fermentation					9:20	In vitro Fermentation	0:20	In vitro Fermentation of Polysaccharides from Aloe vera	0:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	9	from	vera	51:54	arg1	Evaluation					64:73	the Evaluation	60:73	the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids	60:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	9	from	vera	51:54	arg1	Polysaccharides					25:39	Polysaccharides	25:39	Polysaccharides from Aloe vera	25:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	10	theme	Production	103:112	arg1	Fermentation					9:20	In vitro Fermentation	0:20	In vitro Fermentation of Polysaccharides from Aloe vera	0:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	10	theme	Production	103:112	arg1	Evaluation					64:73	the Evaluation	60:73	the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids	60:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	3	11	theme	Aloe	659:662	arg1	vera					664:667	Aloe vera	659:667	Aloe vera	659:667	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	5	12	theme	other	1058:1062	arg1	hand					1064:1067	the other hand	1054:1067	the other hand	1054:1067	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	3	13	from	vera	664:667	arg1	digestion					598:606	in vitro digestion	589:606	in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera	589:667	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	5	14	theme	Aloe	1238:1241	arg1	vera					1243:1246	Aloe vera	1238:1246	Aloe vera	1238:1246	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	2	15	theme	colonic	433:439	arg1	fermentation					441:452	colonic fermentation	433:452	colonic fermentation	433:452	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	3	16	dep	in	717:718	arg1	vitro					720:724	vitro	720:724	vitro	720:724	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	5	17	theme	vera	1243:1246	arg1	use					1231:1233	the possible use	1218:1233	the possible use of Aloe vera and polysaccharide extract as prebiotics	1218:1287	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	18	theme	In	0:1	arg1	Fermentation					9:20	In vitro Fermentation	0:20	In vitro Fermentation of Polysaccharides from Aloe vera	0:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	1	19	theme	prebiotic	175:183	arg1	effects					185:191	prebiotic effects	175:191	prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health	175:301	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	4	20	theme	antioxidant	972:982	arg1	activity					984:991	the antioxidant activity	968:991	the antioxidant activity	968:991	The results showed that the phenolic compounds increased during digestion, but were reduced in fermentation, as a consequence, the antioxidant activity increased significantly in AV and AP after the digestion.					
31591306	0	21	theme	Short	117:121	arg1	Acids					135:139	Short Chain Fatty Acids	117:139	Short Chain Fatty Acids	117:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	3	22	dep	in	589:590	arg1	vitro					592:596	vitro	592:596	vitro	592:596	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	2	23	theme	chronic	410:416	arg1	diseases					418:425	chronic diseases	410:425	chronic diseases	410:425	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	1	24	theme	microbiota	260:269	arg1	species					271:277	microbiota species	260:277	microbiota species	260:277	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	4	25	theme	phenolic	869:876	arg1	compounds					878:886	the phenolic compounds	865:886	the phenolic compounds	865:886	The results showed that the phenolic compounds increased during digestion, but were reduced in fermentation, as a consequence, the antioxidant activity increased significantly in AV and AP after the digestion.					
31591306	2	26	theme	short	463:467	arg1	SCFAs					488:492	SCFAs	488:492	SCFAs	488:492	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	2	26	theme	short	463:467	arg1	acids					481:485	short chain fatty acids	463:485	short chain fatty acids (SCFAs)	463:493	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	2	27	theme	chain	469:473	arg1	SCFAs					488:492	SCFAs	488:492	SCFAs	488:492	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	2	27	theme	chain	469:473	arg1	acids					481:485	short chain fatty acids	463:485	short chain fatty acids (SCFAs)	463:493	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	1	28	theme	species	271:277	arg1	composition					245:255	the composition	241:255	the composition of microbiota species to benefit human health	241:301	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	0	29	theme	Fatty	129:133	arg1	Acids					135:139	Short Chain Fatty Acids	117:139	Short Chain Fatty Acids	117:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	30	from	Evaluation	64:73	arg1	vera					51:54	Aloe vera	46:54	Aloe vera	46:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	5	31	theme	possible	1222:1229	arg1	use					1231:1233	the possible use	1218:1233	the possible use of Aloe vera and polysaccharide extract as prebiotics	1218:1287	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	32	theme	Polysaccharides	25:39	arg1	Fermentation					9:20	In vitro Fermentation	0:20	In vitro Fermentation of Polysaccharides from Aloe vera	0:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	32	theme	Polysaccharides	25:39	arg1	Evaluation					64:73	the Evaluation	60:73	the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids	60:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	5	33	theme	AP	1138:1139	arg1	fibre					1122:1126	fibre	1122:1126	fibre	1122:1126	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	34	theme	Chain	123:127	arg1	Acids					135:139	Short Chain Fatty Acids	117:139	Short Chain Fatty Acids	117:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	3	35	theme	gel	611:613	arg1	digestion					598:606	in vitro digestion	589:606	in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera	589:667	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	36	theme	colonic	726:732	arg1	fermentation					734:745	in vitro colonic fermentation	717:745	in vitro colonic fermentation	717:745	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	37	theme	in	717:718	arg1	fermentation					734:745	in vitro colonic fermentation	717:745	in vitro colonic fermentation	717:745	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	0	38	theme	Aloe	46:49	arg1	vera					51:54	Aloe vera	46:54	Aloe vera	46:54	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	1	39	theme	human	290:294	arg1	health					296:301	human health	290:301	human health	290:301	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	0	40	theme	Acids	135:139	arg1	Activity					90:97	Antioxidant Activity	78:97	Antioxidant Activity	78:97	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	0	40	theme	Acids	135:139	arg1	Production					103:112	Production	103:112	Production of Short Chain Fatty Acids	103:139	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	3	41	theme	polysaccharide	626:639	arg1	AP					650:651	AP	650:651	AP	650:651	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	41	theme	polysaccharide	626:639	arg1	extract					641:647	a polysaccharide extract	624:647	a polysaccharide extract (AP)	624:652	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	42	theme	polyphenolic	559:570	arg1	compounds					572:580	polyphenolic compounds	559:580	polyphenolic compounds	559:580	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	5	43	theme	colon	1086:1090	arg1	fermentation					1092:1103	in vitro colon fermentation	1077:1103	in vitro colon fermentation	1077:1103	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	3	44	theme	extract	641:647	arg1	digestion					598:606	in vitro digestion	589:606	in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera	589:667	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	45	theme	SCFAs	799:803	arg1	production					805:814	SCFAs production	799:814	SCFAs production	799:814	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	1	46	contain	has	171:173	arg1	fibre					165:169	fibre	165:169	fibre	165:169	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	1	46	contain	has	171:173	arg2	effects					185:191	prebiotic effects	175:191	prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health	175:301	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	3	47	theme	present	500:506	arg1	work					508:511	The present work	496:511	The present work	496:511	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	3	48	theme	antioxidant	774:784	arg1	capacity					786:793	antioxidant capacity	774:793	antioxidant capacity	774:793	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	1	49	used	used	205:208	arg2	effects					185:191	prebiotic effects	175:191	prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health	175:301	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.					
31591306	3	50	theme	in	589:590	arg1	digestion					598:606	in vitro digestion	589:606	in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera	589:667	The present work studied the changes produced in the fibre and polyphenolic compounds during in vitro digestion of gel (AV) and a polysaccharide extract (AP) from Aloe vera, after which, these fractions were subjected to in vitro colonic fermentation to evaluate the changes in antioxidant capacity and SCFAs production during the fermentation.					
31591306	5	51	theme	polysaccharide	1252:1265	arg1	extract					1267:1273	polysaccharide extract	1252:1273	polysaccharide extract	1252:1273	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	5	52	theme	gas	1188:1190	arg1	volume					1178:1183	the total volume	1168:1183	the total volume of gas produced	1168:1199	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	5	52	theme	gas	1188:1190	arg1	lactulose					1154:1162	lactulose	1154:1162	lactulose	1154:1162	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	5	53	theme	in	1077:1078	arg1	fermentation					1092:1103	in vitro colon fermentation	1077:1103	in vitro colon fermentation	1077:1103	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	0	54	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids.					
31591306	5	55	theme	extract	1267:1273	arg1	use					1231:1233	the possible use	1218:1233	the possible use of Aloe vera and polysaccharide extract as prebiotics	1218:1287	On the other hand, during in vitro colon fermentation, the unfermented fibre of AV and AP responded as lactulose and the total volume of gas produced, which indicates the possible use of Aloe vera and polysaccharide extract as prebiotics.					
31591306	2	56	theme	Lactobacillus	349:361	arg1	strains					363:369	Bifidobacterium and Lactobacillus strains	329:369	strains	363:369	Prebiotics mostly target Bifidobacterium and Lactobacillus strains, among others, which can fight against chronic diseases since colonic fermentation produces short chain fatty acids (SCFAs).					
31591306	1	57	theme	food	217:220	arg1	industry					222:229	the food industry	213:229	the food industry	213:229	Soluble or fermentable fibre has prebiotic effects that can be used in the food industry to modify the composition of microbiota species to benefit human health.